LOCUS       DAYATN010000001        86334 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.001, whole genome shotgun sequence.
ACCESSION   DAYATN010000001 DAYATN010000000
VERSION     DAYATN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 86334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86334
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.001"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="ACGI8A_000001"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="ACGI8A_000001"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..466
                     /locus_tag="ACGI8A_000002"
     CDS             275..466
                     /locus_tag="ACGI8A_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFH0178018.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            621..2030
                     /locus_tag="ACGI8A_000003"
     CDS             621..2030
                     /locus_tag="ACGI8A_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0178019.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2161..2850
                     /locus_tag="ACGI8A_000004"
     CDS             2161..2850
                     /locus_tag="ACGI8A_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFH0178020.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2853..3788
                     /locus_tag="ACGI8A_000005"
     CDS             2853..3788
                     /locus_tag="ACGI8A_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0178021.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3785..4678
                     /locus_tag="ACGI8A_000006"
     CDS             3785..4678
                     /locus_tag="ACGI8A_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFH0178022.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4756..5502
                     /locus_tag="ACGI8A_000007"
     CDS             4756..5502
                     /locus_tag="ACGI8A_000007"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFH0178023.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5503..6336
                     /gene="rsmI"
                     /locus_tag="ACGI8A_000008"
     CDS             5503..6336
                     /gene="rsmI"
                     /locus_tag="ACGI8A_000008"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFH0178024.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEIRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6359..7063
                     /locus_tag="ACGI8A_000009"
     CDS             6359..7063
                     /locus_tag="ACGI8A_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFH0178025.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7251..7679)
                     /locus_tag="ACGI8A_000010"
     CDS             complement(7251..7679)
                     /locus_tag="ACGI8A_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFH0178026.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7716..9146)
                     /locus_tag="ACGI8A_000011"
     CDS             complement(7716..9146)
                     /locus_tag="ACGI8A_000011"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFH0178027.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9278..9862
                     /locus_tag="ACGI8A_000012"
     CDS             9278..9862
                     /locus_tag="ACGI8A_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HFH0178028.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9878..10399
                     /locus_tag="ACGI8A_000013"
     CDS             9878..10399
                     /locus_tag="ACGI8A_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HFH0178029.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10465..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10795..12732
                     /gene="metG"
                     /locus_tag="ACGI8A_000014"
     CDS             10795..12732
                     /gene="metG"
                     /locus_tag="ACGI8A_000014"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFH0178030.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12762..13532
                     /locus_tag="ACGI8A_000015"
     CDS             12762..13532
                     /locus_tag="ACGI8A_000015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFH0178031.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13870..14301
                     /gene="phnG"
                     /locus_tag="ACGI8A_000016"
     CDS             13870..14301
                     /gene="phnG"
                     /locus_tag="ACGI8A_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFH0178032.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14316..14891
                     /gene="phnH"
                     /locus_tag="ACGI8A_000017"
     CDS             14316..14891
                     /gene="phnH"
                     /locus_tag="ACGI8A_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFH0178033.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTNLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14894..16009
                     /locus_tag="ACGI8A_000018"
     CDS             14894..16009
                     /locus_tag="ACGI8A_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HFH0178034.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRAAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKIKHPIYEDEEITIGDMLVTECEIVSKASQNK
                     TENSKNNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIKKFEEVSLGDI"
     gene            15999..16868
                     /locus_tag="ACGI8A_000019"
     CDS             15999..16868
                     /locus_tag="ACGI8A_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFH0178035.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16861..17727
                     /locus_tag="ACGI8A_000020"
     CDS             16861..17727
                     /locus_tag="ACGI8A_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178036.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQREFKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17768..18481
                     /locus_tag="ACGI8A_000021"
     CDS             17768..18481
                     /locus_tag="ACGI8A_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFH0178037.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18482..19651
                     /locus_tag="ACGI8A_000022"
     CDS             18482..19651
                     /locus_tag="ACGI8A_000022"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFH0178038.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19655..20491
                     /locus_tag="ACGI8A_000023"
     CDS             19655..20491
                     /locus_tag="ACGI8A_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFH0178039.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20492..21487
                     /locus_tag="ACGI8A_000024"
     CDS             20492..21487
                     /locus_tag="ACGI8A_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178040.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21505..23193
                     /locus_tag="ACGI8A_000025"
     CDS             21505..23193
                     /locus_tag="ACGI8A_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HFH0178041.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23205..24272
                     /locus_tag="ACGI8A_000026"
     CDS             23205..24272
                     /locus_tag="ACGI8A_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0178042.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24756..24872
                     /locus_tag="ACGI8A_000027"
     CDS             24756..24872
                     /locus_tag="ACGI8A_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HFH0178043.1"
                     /translation="MDDDINMVAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            25036..26103
                     /locus_tag="ACGI8A_000028"
     CDS             25036..26103
                     /locus_tag="ACGI8A_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0178044.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            26295..26825
                     /gene="rnmV"
                     /locus_tag="ACGI8A_000029"
     CDS             26295..26825
                     /gene="rnmV"
                     /locus_tag="ACGI8A_000029"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFH0178045.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            26996..27865
                     /gene="rsmA"
                     /locus_tag="ACGI8A_000030"
     CDS             26996..27865
                     /gene="rsmA"
                     /locus_tag="ACGI8A_000030"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFH0178046.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            27931..29193
                     /locus_tag="ACGI8A_000031"
     CDS             27931..29193
                     /locus_tag="ACGI8A_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178047.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            29344..30459
                     /locus_tag="ACGI8A_000032"
     CDS             29344..30459
                     /locus_tag="ACGI8A_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFH0178048.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            30645..31541
                     /locus_tag="ACGI8A_000033"
     CDS             30645..31541
                     /locus_tag="ACGI8A_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFH0178049.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            31611..32408
                     /locus_tag="ACGI8A_000034"
     CDS             31611..32408
                     /locus_tag="ACGI8A_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178050.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(32463..33815)
                     /gene="murC"
                     /locus_tag="ACGI8A_000035"
     CDS             complement(32463..33815)
                     /gene="murC"
                     /locus_tag="ACGI8A_000035"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFH0178051.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            34034..34912
                     /gene="purR"
                     /locus_tag="ACGI8A_000036"
     CDS             34034..34912
                     /gene="purR"
                     /locus_tag="ACGI8A_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFH0178052.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            35068..35346
                     /gene="spoVG"
                     /locus_tag="ACGI8A_000037"
     CDS             35068..35346
                     /gene="spoVG"
                     /locus_tag="ACGI8A_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HFH0178053.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            35559..36938
                     /gene="glmU"
                     /locus_tag="ACGI8A_000038"
     CDS             35559..36938
                     /gene="glmU"
                     /locus_tag="ACGI8A_000038"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFH0178054.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            37033..37983
                     /locus_tag="ACGI8A_000039"
     CDS             37033..37983
                     /locus_tag="ACGI8A_000039"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFH0178055.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      38821..38906
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            39068..39589
                     /locus_tag="ACGI8A_000040"
     CDS             39068..39589
                     /locus_tag="ACGI8A_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFH0178056.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSATTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            39740..41416
                     /locus_tag="ACGI8A_000041"
     CDS             39740..41416
                     /locus_tag="ACGI8A_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HFH0178057.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            41428..42636
                     /locus_tag="ACGI8A_000042"
     CDS             41428..42636
                     /locus_tag="ACGI8A_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFH0178058.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            42651..44363
                     /locus_tag="ACGI8A_000043"
     CDS             42651..44363
                     /locus_tag="ACGI8A_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFH0178059.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            44356..45264
                     /gene="pilO"
                     /locus_tag="ACGI8A_000044"
     CDS             44356..45264
                     /gene="pilO"
                     /locus_tag="ACGI8A_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFH0178060.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            45291..45857
                     /locus_tag="ACGI8A_000045"
     CDS             45291..45857
                     /locus_tag="ACGI8A_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFH0178061.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            45894..46421
                     /locus_tag="ACGI8A_000046"
     CDS             45894..46421
                     /locus_tag="ACGI8A_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFH0178062.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            46442..47980
                     /locus_tag="ACGI8A_000047"
     CDS             46442..47980
                     /locus_tag="ACGI8A_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178063.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRDISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLEKLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            48080..49162
                     /locus_tag="ACGI8A_000048"
     CDS             48080..49162
                     /locus_tag="ACGI8A_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFH0178064.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQNPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            49143..49925
                     /locus_tag="ACGI8A_000049"
     CDS             49143..49925
                     /locus_tag="ACGI8A_000049"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFH0178065.1"
                     /translation="MDIIIVNLYVFMVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            50207..50677
                     /locus_tag="ACGI8A_000050"
     CDS             50207..50677
                     /locus_tag="ACGI8A_000050"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFH0178066.1"
                     /translation="MVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNIVQ
                     FDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFILA
                     FLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            50691..51251
                     /gene="pth"
                     /locus_tag="ACGI8A_000051"
     CDS             50691..51251
                     /gene="pth"
                     /locus_tag="ACGI8A_000051"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFH0178067.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            51268..54654
                     /gene="mfd"
                     /locus_tag="ACGI8A_000052"
     CDS             51268..54654
                     /gene="mfd"
                     /locus_tag="ACGI8A_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFH0178068.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            54685..55680
                     /locus_tag="ACGI8A_000053"
     CDS             54685..55680
                     /locus_tag="ACGI8A_000053"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFH0178069.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            55804..56343
                     /gene="spoVT"
                     /locus_tag="ACGI8A_000054"
     CDS             55804..56343
                     /gene="spoVT"
                     /locus_tag="ACGI8A_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HFH0178070.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            56463..58076
                     /locus_tag="ACGI8A_000055"
     CDS             56463..58076
                     /locus_tag="ACGI8A_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFH0178071.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            58183..59631
                     /gene="mazG"
                     /locus_tag="ACGI8A_000056"
     CDS             58183..59631
                     /gene="mazG"
                     /locus_tag="ACGI8A_000056"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFH0178072.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            59720..59998
                     /locus_tag="ACGI8A_000057"
     CDS             59720..59998
                     /locus_tag="ACGI8A_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFH0178073.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            60070..60312
                     /locus_tag="ACGI8A_000058"
     CDS             60070..60312
                     /locus_tag="ACGI8A_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFH0178074.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            60374..60628
                     /locus_tag="ACGI8A_000059"
     CDS             60374..60628
                     /locus_tag="ACGI8A_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HFH0178075.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            60629..61162
                     /gene="yabQ"
                     /locus_tag="ACGI8A_000060"
     CDS             60629..61162
                     /gene="yabQ"
                     /locus_tag="ACGI8A_000060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HFH0178076.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            61168..61452
                     /locus_tag="ACGI8A_000061"
     CDS             61168..61452
                     /locus_tag="ACGI8A_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFH0178077.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            61565..62485
                     /locus_tag="ACGI8A_000062"
     CDS             61565..62485
                     /locus_tag="ACGI8A_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HFH0178078.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDKGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            62575..64947
                     /gene="spoIIE"
                     /locus_tag="ACGI8A_000063"
     CDS             62575..64947
                     /gene="spoIIE"
                     /locus_tag="ACGI8A_000063"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HFH0178079.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            65298..67190
                     /gene="pepF"
                     /locus_tag="ACGI8A_000064"
     CDS             65298..67190
                     /gene="pepF"
                     /locus_tag="ACGI8A_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFH0178080.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            67304..68269
                     /gene="galU"
                     /locus_tag="ACGI8A_000065"
     CDS             67304..68269
                     /gene="galU"
                     /locus_tag="ACGI8A_000065"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFH0178081.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            68769..70373
                     /gene="rho"
                     /locus_tag="ACGI8A_000066"
     CDS             68769..70373
                     /gene="rho"
                     /locus_tag="ACGI8A_000066"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFH0178082.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            70526..70726
                     /gene="rpmE"
                     /locus_tag="ACGI8A_000067"
     CDS             70526..70726
                     /gene="rpmE"
                     /locus_tag="ACGI8A_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFH0178083.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            70871..71764
                     /locus_tag="ACGI8A_000068"
     CDS             70871..71764
                     /locus_tag="ACGI8A_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HFH0178084.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            71782..72630
                     /gene="prmC"
                     /locus_tag="ACGI8A_000069"
     CDS             71782..72630
                     /gene="prmC"
                     /locus_tag="ACGI8A_000069"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFH0178085.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            72674..73738
                     /gene="prfA"
                     /locus_tag="ACGI8A_000070"
     CDS             72674..73738
                     /gene="prfA"
                     /locus_tag="ACGI8A_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFH0178086.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            73867..74547
                     /locus_tag="ACGI8A_000071"
     CDS             73867..74547
                     /locus_tag="ACGI8A_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFH0178087.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            74576..75616
                     /locus_tag="ACGI8A_000072"
     CDS             74576..75616
                     /locus_tag="ACGI8A_000072"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFH0178088.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            75633..76082
                     /locus_tag="ACGI8A_000073"
     CDS             75633..76082
                     /locus_tag="ACGI8A_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HFH0178089.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            76099..76551
                     /gene="rpiB"
                     /locus_tag="ACGI8A_000074"
     CDS             76099..76551
                     /gene="rpiB"
                     /locus_tag="ACGI8A_000074"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HFH0178090.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            76587..77216
                     /gene="upp"
                     /locus_tag="ACGI8A_000075"
     CDS             76587..77216
                     /gene="upp"
                     /locus_tag="ACGI8A_000075"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFH0178091.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            77511..78326
                     /gene="yqeB"
                     /locus_tag="ACGI8A_000076"
     CDS             77511..78326
                     /gene="yqeB"
                     /locus_tag="ACGI8A_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFH0178092.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            78552..78989
                     /locus_tag="ACGI8A_000077"
     CDS             78552..78989
                     /locus_tag="ACGI8A_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HFH0178093.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            79174..79401
                     /locus_tag="ACGI8A_000078"
     CDS             79174..79401
                     /locus_tag="ACGI8A_000078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HFH0178094.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            79406..79792
                     /locus_tag="ACGI8A_000079"
     CDS             79406..79792
                     /locus_tag="ACGI8A_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFH0178095.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            79795..80499
                     /gene="atpB"
                     /locus_tag="ACGI8A_000080"
     CDS             79795..80499
                     /gene="atpB"
                     /locus_tag="ACGI8A_000080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFH0178096.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            80568..80828
                     /gene="atpE"
                     /locus_tag="ACGI8A_000081"
     CDS             80568..80828
                     /gene="atpE"
                     /locus_tag="ACGI8A_000081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFH0178097.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            80951..81448
                     /gene="atpF"
                     /locus_tag="ACGI8A_000082"
     CDS             80951..81448
                     /gene="atpF"
                     /locus_tag="ACGI8A_000082"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFH0178098.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            81445..81990
                     /locus_tag="ACGI8A_000083"
     CDS             81445..81990
                     /locus_tag="ACGI8A_000083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFH0178099.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            82007..83509
                     /gene="atpA"
                     /locus_tag="ACGI8A_000084"
     CDS             82007..83509
                     /gene="atpA"
                     /locus_tag="ACGI8A_000084"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFH0178100.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            83537..84415
                     /gene="atpG"
                     /locus_tag="ACGI8A_000085"
     CDS             83537..84415
                     /gene="atpG"
                     /locus_tag="ACGI8A_000085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HFH0178101.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            84428..85822
                     /gene="atpD"
                     /locus_tag="ACGI8A_000086"
     CDS             84428..85822
                     /gene="atpD"
                     /locus_tag="ACGI8A_000086"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFH0178102.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            85825..86085
                     /gene="atpC"
                     /locus_tag="ACGI8A_000087"
     CDS             85825..86085
                     /gene="atpC"
                     /locus_tag="ACGI8A_000087"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HFH0178103.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        34780 a         9323 c        15856 g        26375 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgtaattt tttttatttt ttgtcaaaaa
      181 aaacaaaaaa tacgtatttc ttttttcatt tcgggaaaac taaacacagg aattatggac
      241 tttataaaaa tttaattctt gaggagtgat ataagtgaaa aaagttgctt taattttagc
      301 tataataggt gcgcttaact ggggtgcaat tggtattcta ggtacagact taataggtag
      361 tatctttggt ggaacatatg aaatggtaag tagaataata tacttcattg taggtttagc
      421 tggattatac ttaataccaa gtttaatgtc tgatgataga gaataattta tgcttttatg
      481 ttaaatacct cattctaaat gtttagaatg aggtatttaa ttttataaaa atattaattt
      541 gtttcttttt taatggtaaa atagataaga attgaagttt taaaaagaag tattatgtaa
      601 aaaaggagta ctattaatat atgtttatta ttaatgaatt aaataagatt gtaaatgaag
      661 aagtaatatc ttttcatatg cctggtcata aaaagggaca ggtttataaa aagttaggat
      721 ataaagatat actaaaaaat ttgtataaaa tggatacaac tgagatatta ggaacagata
      781 atttacactt tccggaagga attataaaaa attcacaaaa aaaagcatca agagtattta
      841 aaagtgatta tacttgctat ttagtaaatg gtagtagctg tggaatacaa tctgcaataa
      901 tgtcagtatg tagtcctaaa agtaaaataa taacaaatag agactgtcat cagtctgtta
      961 taaatggatg tattttagga gatatagata tagagtatgt gccttgtgag ataagtgaag
     1021 agacaaatgt cttaaaaggg atatctataa gtaatgtgat tgatataata gataaaaact
     1081 tggatgcaaa agcagtactg cttacttatc ctacatatta tggaatgaca tatgatttag
     1141 aatacatatg taattatgca catagcaaaa agatggtagt gatagtagat gaagcacatg
     1201 gtgcgcattt agggcttagc gagaggcttc ctaaaacagc tcttgagcaa ggcgcagata
     1261 tagttgtaca gagtacacat aaaactcttc cagcatttac acaatcgtca atgatacata
     1321 tacagggaga ccgagttgat atagataaaa tattgaacat gttaagaatt acagaatctt
     1381 caagcccttc atatttatta ttatcttctc tggaattagc agtagatata tatgaaaata
     1441 aaggaaaaga attaatggaa gagcttttaa ataatattat aactttcaaa aataaagttg
     1501 attcaaatat taatatatac gacacaaatg ataaaacaaa aatatttata tcatctaaaa
     1561 atatgggttt aacaggttat gaactagaaa atattttaag aaacaaatat aacatacaag
     1621 tggagttatc taattattat ggagttcttt taatatgtac aataggaaat actgttgagg
     1681 atttctctag tttagaaaca gcattaaaca atattgtaaa ggaagacttt aaatttgaaa
     1741 agttagaaaa taaaaaatac cctgttgtta ttccaaagaa aatattaaca cctagagaag
     1801 cattttataa aactaaaaaa agtgttaaaa tatatgacag cataggtaaa gtttgtggtg
     1861 aatgtatagt tccataccca cctggaataa gtgtaatatc tcctggtgaa gtaataagta
     1921 aagaaataat agactatcta atgttttgtc attctaaagg catgattgtg agtggattga
     1981 aagatgtaga tttaaatttt atacaaataa tagatttaga agacatttaa aaattatgat
     2041 ataaaagtag atagaagtag atagtttaaa attgtgaaga gaaataaata gactgcttat
     2101 atagagctat ctaataaaag tactaaaaaa gtaaaaagtg tttgtatgga ggatatgaaa
     2161 atgaaaggta agttaataat tatagagagt ggttcagatg caagtggaaa ggctactcag
     2221 acagaaatat tatatgaaag attaaaaaaa gaaggctata tgattaaaaa ggtagaatat
     2281 ccaaattatg aatctccatc atctgccttg gtgaaaatgt acctaaaagg agactttggt
     2341 aaaaatgcat cagatgtaga cccatatata gcatcaactt tttttacagc tgataggtat
     2401 gcatcattta aaaccgattg ggaggagttt tacaatgaag gtggaataat tatcgcagat
     2461 agatatacaa cctctaatat ggtacatcag gcctctaaaa tggatatttg tgatagagaa
     2521 gagtatttaa attggttagc tgattatgaa tttaatttat ttaagatacc acaacctgat
     2581 tgtgttatat ttttagatgt gcctattgaa tttagtaaaa agcttatgga gaatagaaaa
     2641 aataaaatta caggggaaga aaaaaaggat attcatgaaa gtgacataga gtatttagag
     2701 aaatcttaca acaatgcttt atatatagca gataaatatg attggaaaaa aataaattgt
     2761 gttttagata acaaattaag aagtattgaa agtatacata atgaaattta taaaatagtt
     2821 cttgattcaa taaaacctat ggagagataa atatgtattt tgaaaatatt atagggcaag
     2881 attttgcaaa gaaatattta acaaattcta taaagaaaaa taaacttaat aatgcgtata
     2941 tgtttgaagg aatggatgga ataggaaaaa agaaatttgc agatgaatta tcaaaactat
     3001 tacttgatta tgaaaatctc gaaaatagtc cagactatgt actcataaaa ccagatggaa
     3061 atagtataaa aatagctcaa atcagaaatt tacaatcaga tatagtaata agacctcata
     3121 aagactataa aatatatata atcaataatg ctgaaaaaat gactgtggaa gctcaaaatg
     3181 cacttttaaa gacgctagag gaacctccaa actatgctat aataatctta gtcacaaaca
     3241 acaaggaatc tttattagaa actataaaat ctagatgtga tataataaag ttctcaccta
     3301 taccaattga agacttaaaa cgttatttga taaatacagg tatagaagaa gaaagagctc
     3361 aattattggc tattttttcg agaggaagta ttgagaatgc tctaaatcta tctcagtctt
     3421 ctgagttttc aatgatgaga gaagatatac aacagtatat tcagataatg ttagataaaa
     3481 acatagtgga gattttaaat ataccaaata atatggaaaa atatagaggt aagattatag
     3541 ctttattaga tatgatgata aactattttc gagatatcat cctattaaaa gaaaatgtaa
     3601 acaagaacat gttaataaat gtagataagc tagttttcat tcaaaatatg agtggaaaaa
     3661 ttagttattc tcaattgtcc aagattattg atataataga agatgcaaag agtaaaataa
     3721 aaagcaattg caatttcaat ataagcatac aagttatgtc tttaaatata tacgaggtga
     3781 ttaaatgata aagatagtag gtataaggtt taaaagtgct gggaaaatat attattttga
     3841 ccctgttgat tttaatatag agcaagatat ggatgtagtt gtagaaactg caagaggctt
     3901 ggagtatggg aaagtagtcg ttggtccaaa agatatggat gaaagcgaat tagtttctcc
     3961 tttgaagcca attataagaa tagctacaga agaagataaa aaagtatatc tagaaaataa
     4021 ggaaaaggca aaagaaactt ttgagttgtg ccaacaaaaa ataaaagaac atgagttaac
     4081 aatgttttta atagattgtg agtatacatt tgataggaat aagttgattt tttactttac
     4141 agcagaagga agaatagatt ttagagaatt ggtaaaagac ttagctgcta tatttaaaac
     4201 tagaatagaa cttagacaaa taggtgtcag agatgaagct aaatctatag gaggtctagg
     4261 gccttgtggt agaaaacttt gttgttcatc ttggttaggt gattttcaac cagtttcaat
     4321 aaaaatggca aaagaccaaa gtctatctct aaatccaaca aaaatatctg gaatatgtgg
     4381 gagattattc tgttgcttaa aatatgagca tgatgtatat agtgaagcaa tagaaaaaat
     4441 gcctgttgta ggtgcattgg tgcaatcaga tgatggaaaa ggaaaagttg tagaagtgaa
     4501 tcctttatta gaacaaatta aagtggaatt tcaagacaag acagtaaaga tatacacaag
     4561 agaagatata aaaatactta gagagcctaa aaaatgtgat ggctgtggtg gtaaatgtaa
     4621 ggataatgat ggattagatg aagccacact aagagagcta aagaaattag aagattaaat
     4681 ttaaggtagg gcttttccta ccttttttaa agcaattaat gcttaaagtt attaatattt
     4741 gaggagaatt tatatatgca attaaaagat tcagaaagaa tagatgattt acaattaaaa
     4801 ggccttaaaa ttatacaaga tactaatggt ttttgttttg gtatagatgc agttttatta
     4861 gccaatttta ctaaaataaa aaaagatgcc aaagtagtag atttgggtac tggaactgga
     4921 attataccta ttttgatagc tggaaaaagt gaagctaaaa aaattattgg cgtggaaata
     4981 caagaagatg tatatgaaat ggcaactcgt tctataaaat taaatggatt agaagaaaga
     5041 gttgagatta taaatgaaga tataaaatac atagacaaag tcttagacat aaatggttac
     5101 catgtagtca catcaaatcc tccatatatg catattgatg gaataaaaaa tccaaatgat
     5161 aaaaaagcaa tatcaagaca tgaagtaagg tgcaatttgg aagatgtaat tagagctgca
     5221 tccagattgg ttatgcctag aggaaaattc ttcatgatac atagaccaac aagattggtt
     5281 gatataataa ctttagggag aaaatataaa ttagaaccaa aggttattca atttgtgcat
     5341 ccaagacctc aaaaagcacc gaacttagtt ttggttcaat ttgtaaaaga tggaaggcca
     5401 gagcttaaaa tattggaccc attgtatgtt tatggagaag atgggaatta tacaaaagaa
     5461 ttaaaagcaa tatataataa taaagatata ggagagttgt aaatgagtgg gaaattgtac
     5521 atatgtccaa caccaatagg aaacctagaa gatataacat atagaacttt aagaacatta
     5581 aaagaagtag acttgattgc agcagaagat acaagacata gtataaaact tttaaatcat
     5641 tttgaaatct caaaaccact aactagttat catgaacata ataaggattc aaagggagat
     5701 tatttgataa ataaactaat tgatggtgaa aatatagctc tgataagtga tgcaggtatg
     5761 cctggtatat ctgacccagg agaagagata ataaaacaag ctatacaaaa taacatagag
     5821 atagaagtat tgccaggtgc aacagccttt gtaacggctt tagttggctc tggtatggac
     5881 acacatagat ttgtttttga gggcttttta gatagagata aaaaagttag gaaagcacaa
     5941 ttggaagaat taaaggaaga aagtagaact ataattttct atgaatcacc acatagattg
     6001 aaggatacac taaaagatat gcttaaaatt cttggtaata ggaatatttc tataaataga
     6061 gaacttacaa aaaaatatca agaaataata agagaaaata tagaaactac cataaaaata
     6121 tttgaggaaa aagaaataag aggagaattt gtattaatag tagagggttt tcatggtgag
     6181 aagtcaaaga aagaaaatta tgatgggctt acagataggg aatatgtttt aaaactaatt
     6241 gaaagtggta tgaacaaaaa agatgcaata aaaatagttt gtaaagatag aaagttaaag
     6301 aaagatgtgg tttataaaca agttttagat ttataaaacc tgaatctgag gtgattttat
     6361 ggaaataggg aaaattataa tagagagttt aaagagggaa gatacacata aaatatttat
     6421 agaattagat aataagcatg aattagaaaa aataattcct aaaataagct gtatgaagtc
     6481 tgtaggtgaa tgtaaatatc atgtagtaaa ttgttttgaa cattctataa acgcattaaa
     6541 agaattagaa atagtattaa atgataagga tttttttcca atacatctaa gaaaacatgt
     6601 gtcaaactat ttaaacacat atattgaaga aggaataaat aagttacatg tattaaagct
     6661 gggaacattt ttacatgata taggtaagcc tgacagtatg accttagatg aaaccggaag
     6721 agttcatttc acaaatcatg aaaaaatagg tgctcaaata attgacaata tgggaataaa
     6781 gttagagtta tcaacagaga catatagact tataagtaaa tatgtaagat atcatatgat
     6841 acttttgtct ctatataaga aaaatgattt gagtaggaaa gaattaatta atgtatttaa
     6901 cttagtagat gaggacacta taggtgtaat aatgcttggg tatgcagata tagtgtctac
     6961 agtaaaatta ttgggcaaga gtggagaagt cagtgtgtta aagacatata tggaatatat
     7021 tttaacaaat tacttgtata agagtaatta tacacatgta taaatttagt taatataatt
     7081 gagttttaga tgattaattg aagtttgaaa tatacggatt tcaaaaacat aaaagaagtt
     7141 gtctttgttg taaaattatg aaagacaact ttttttatat caaaaattga aattttatta
     7201 aggatactta atggtctatt aattaataaa ccattagtat ttaatttata ttacaatatt
     7261 gattttgctt gtattggcgt tgatagtata acagaggtct tagtagaacc aattttcttt
     7321 atagtatcaa tcacattttc taactcgtac atatctttta caattacttt taaaagtaag
     7381 caatcatcac ctgttatatg gtgacattct acaatcctag ggtcctttgc agctgactca
     7441 ataaattctg tatatccatt gcttggaaga gaaatatgaa taaatgcctt tataactctg
     7501 cctaatgaat ctgggttgac aatagcttta tatccttcta taacaccaga ctcttctaat
     7561 cttttgactc tttctgaaac tgcaggagaa gttaaaccaa ctatttttcc taagtccttc
     7621 atagaaattc tcccatcatc ctgtaaaatt tctatgattc tgtaatctgt aacatccata
     7681 aataaacagt cccccttaaa taaaaataat taatgctata aaactttttt agaatctatc
     7741 atttgtgttt ttaaatacca aagatttcta gataaatcaa ccttataaat gtgtggtttt
     7801 gaaagtcttt tgtattgttc ccagatagta cttaattcat attgtacatg attttttatt
     7861 tcaagaacag cttttttatt atatttgcat ctgcctttta tatacaatgg tttaagaagc
     7921 tctttaactt tgtaatttgt aaataccttc gttttccatg tataagtagg atggaagatt
     7981 tctaaaggtt tagactcatc tataacttca tcatgtaaca taatcaaatc tgcctctgcc
     8041 ttattgtctt cgttatatat tcttataact ttcttgtaac caggattatt tattttttct
     8101 ggattctcag aaatctttat cttaggttca agaactcctt tttcacaaga tgcagctaat
     8161 ttataaactc ctccaagaga aggtgaatcg aaagatgtaa tgagctttgt acctactccc
     8221 catgaattta tagtagctcc ttctgcttta agtgatgtta tggtatattc gtctaggtca
     8281 ttagatgcag tgatgctgat atctgaaaag ccagcattat ctaattgttt tttagcttct
     8341 ttagataaat aagctaaatc tccagagtca agtcttatac ccataggttt gtatcctttt
     8401 tcagaaatat ttttgaatac ttttattgca ttaggaactc cactgtttaa tacatcataa
     8461 gtatctacaa gtagtaggca tttatctgga taaacatcag catatgcttg gaatgcttca
     8521 agttcattat caaatttttg tacccaactg tgagcttgtg taccaattat aggtatatca
     8581 aacattttcc ctgctagtac gtttgcagtt ccagcacaac caccaactac agcagcccta
     8641 gccccatata tccctgcatc tggaccttgt gcacgacgta atccaaattc aaatacaggg
     8701 tctccttgag ctgcaaagca aactctagag gctttagtag caataagaga ctgaaaatta
     8761 actattgtta acaatgctgt ttcaataagt tgagcttgat ataaaggtgc cttaacagta
     8821 attaatggct cattagggaa catgatagtt ccttcctcaa cagcatatat atctcctgtg
     8881 aatttaaatt cttttaaaaa ttttaagaac ttgtcagaaa ataaatttaa gttttttaag
     8941 tactctaaat cttcatctga aaaatgaaga ttatctatgt attcaacaac ctgatcaata
     9001 ccacaaacta tagtataccc tccatcacaa gcgttttttc taaaaaacat atcaaataca
     9061 acaatgtctt catgaatatt cttttcaaaa tagccattta acatagtaag ctgatataaa
     9121 tcagtaagaa gtgttagatt tctcattatt tctctcctta attattatta aatattaaaa
     9181 attgtaaaaa ctcccacact tatactaaaa cataatatag tacaactttc aagtataaag
     9241 tattacctaa ctatcataaa attatttaaa gaggaggatg attataatag gaaaaatatg
     9301 gttttatatg gtatcgatag gaataatagg aagcttgatt tctaaaaata tgggtgaatt
     9361 aaataaagtt atattaagtg aaacatcaaa aggtatagag tttgcaataa gtctagctgg
     9421 tatcatggca ctctggatgg ggataatgaa tatcgcaaaa gaatcaggtt taattgaaaa
     9481 gataggtcaa aaactaaatc ctataatgag aagattattt ccttctattc caaaaggtca
     9541 taaagcaatg tcttatatag ttatgaatat tgctttaaat atgttgggtg ctggaaatgg
     9601 tgcaacagca tttggtttaa aagcaatgag tgagcttcaa actttgaata acaagaaaga
     9661 tacagccact aatgatatga ttatgtttat ggtaatcaat atatcatcta tacaaataat
     9721 tccatttacc ataatcaaac taaggatgga tatgggggct caaaatccaa gtgaaataat
     9781 ttttacgact ttatttgcaa ccataatatc tacagcagta gcaataataa catgtaaatt
     9841 ttttcaaaga agatatagat aaaggggatg tagatacatg gagatatttt caaacttact
     9901 tataccaata ataattctgt acatagtaat ttatggtaaa tataaaaaaa tagatgtgta
     9961 tgatagtttt gtaaaaggag caattgatgg attaaaagct gcatgggata tcctaccata
    10021 tataataggt atatttttgg caataggaat attcaaaact ggtaaaggtc ttgatatgtt
    10081 agaatggcta tttacaccaa tagcaaatat gatgagtata ccaaaagaat taataggtct
    10141 aataagtgta aaacctttat ctggaagtgg tgctttaggg atgtacagtg aacttgcaaa
    10201 tagagttgga ataggaagtt tagtagaaaa aatgggtgct acgattgtag gttcttctga
    10261 gactattttt tacacaatgg caatatacta tggaagtctg aagataaaaa atactagaca
    10321 tacattatca tgtgctatga ttagccatgt tgcaggtgtc atagcagctg tttttatttg
    10381 ctatgtaata tttgtataat ttgttgacaa tatgggaaaa attttataat atagtgatta
    10441 tatagaaaca aatagtaata caaattacaa gctatgaaaa gaagagtaaa taaaggtttt
    10501 ttctatcaga gagctaggtt aggtgaaagc tagtgtcaaa agctttattg aagatggtct
    10561 tggagctttt tatctgagca agtgtgctag ggtaaaacgg ttggacacgt tatagtctac
    10621 taaggtatct ataaaatgtt tctacatttc atgtgaaaca taactattat agatgaatta
    10681 gggtggtacc gtgaataatc tcgcccctat gttaactcat aggaggcgat tttttattat
    10741 ttaaatgaaa gaatcatatg attacgaacg ataaaaaatt aggaggtaaa taacatgagc
    10801 aaaccgagtt tttatgtaac aacaccaata tactatccaa gtggaggatt acacatagga
    10861 catacttatt caacagtagc tgcagataca atagctagat tcaagcgttt ctgtggatat
    10921 gatgtaaagt tcttaacagg aacagatgag catggtgaaa aaattcaaaa gaaagccata
    10981 gaacaaggaa tgtcagagat agagtatctt gatggaatga taaaagatat aaaggcctta
    11041 tggaatacta tggatatatc ttatgatgat tttataagaa ctacagaaaa aagacataca
    11101 gacataatac aaaagatatt tactaaacta tatgagcaag gtgatatata taaaggtgaa
    11161 tatgaaggta gatactgtac tccttgtgag agtttctgga cagaatctca attactagaa
    11221 ggaaataaat gccctgactg tggaagagaa acatatttag taaaagaaga atcttatttc
    11281 tttagattat ctaaatatga agacagatta aaagagttat ttaaagatga tagtttctgt
    11341 tttcctgctg ctagaaaaaa tgaaatggtg gcaaactttt tagacaaagg attagaagat
    11401 ttaagcgtaa ctagaacgac ttttgactgg ggtataaaag ttccttttga tgaaaaacat
    11461 gtaatttatg tgtgggtaga tgctttatgt aactatataa cagcattagg ttatatgact
    11521 gataatgatg aagaatttaa aaaatattgg cctgccaatg ttcaaatagt aggtaaagaa
    11581 atagtgagat tccatacaat aatatggcca gcgcttttaa tggctttagg tctagaagtt
    11641 ccaaaacaag tatttggaca tggatggata ctatttgctg atgataaaat gagtaaatca
    11701 aagggtaatg tggtttatcc agaacctata atagaaagat atggaataga cacacttaag
    11761 tacttcttat taagagagtt tgcatttggg caagatggaa gttatactca tagaaatttt
    11821 gtaacgagaa taaattatga tttagcaaat gatttaggaa acttaataag tagaactgta
    11881 gctatggttg aaaaatacaa taatggtata ataccaacag caaaagtttc aactgatttt
    11941 gatgctgact taaaagaaca agctgtttca actagagaaa actttgaagc tgaaatggat
    12001 aaaatgcaat tccatgaagc attagagagt gtatggaaat tagttagaag aactaacaaa
    12061 tatatagatg aaactatgcc ttgggcacta gctaaagatg aaacgaagaa agatgaatta
    12121 gacacagttt tatataattt atgtgaatct ataagaataa tcgcaacact aataaatcct
    12181 ataatgaatg agactgctaa taaaatatat gaacatatag gaataaaagg tcaagatgat
    12241 ataactacat gggaaagtac aaaaacattt ggtcttattg gagaaaatgt aaaagtattt
    12301 aaaggtgaac ctttattccc aagattagat gtagaaaaag agatagaaga attaactaaa
    12361 atgttctctg ggaaacctcc agtagaagaa aaacctttag agcataaaga agaaattact
    12421 attgatgatt tagacaaaat agagcttaga gtagggaaaa taataagctg tgaaaaacat
    12481 ccaaaagcaa ataaattatt agtatcacaa gttaagattg gaccagaaac aagacaaata
    12541 gtatcaggaa tagctgaata ctataagcca gaggatttag taggaaaaga ggttacagtg
    12601 gtatgtaact taaaaccagt taaattaaga ggtgtagaat ctcaaggtat gatattagca
    12661 gcaggtgatg atggagaccc atatgtttta ccatttacac aaggggctaa agatggatgc
    12721 gaggttcgtt agtaggtaat taaatagtaa ggagattaaa gatgttattt gactcacatg
    12781 cacatttaaa tgatgaaagt tttgatgaag atagagatga acttattggt tcattaaaag
    12841 ataaaggtgt agatttggtt gtaaatccag gagcagatat agagacgtcg ataacagcaa
    12901 ttgaacttgc gaaaaaatac gactttatat attcagcagt tggggtacat ccacatgatg
    12961 tatcaaaact agatgataca gcaatagaaa ctctaagaaa acttgcaact gagaatgaaa
    13021 aagttgtagc tattggagag attggattag attattatta tgattattct ccaagagaag
    13081 aacaaaaaga gtggtttaag aagcaaattg aattagctaa tgagctaaaa cttccaataa
    13141 taatacatga tagagatgca catggagata cttttgagat aataaagaat actaaaaatc
    13201 ctgaaatagg atgtgtactt cattgttata gtgggaatgt agagttagct agagaatatg
    13261 ttaagatggg ttgttatata tcaattccag gcacggttac ttttaagaat aataaaaaaa
    13321 ctagagaagt agtaagagaa atacctctag aaagattatt catagagact gattcaccat
    13381 atatgtcacc agagccacat agaggaaaga gaaacaatcc ttcccaagta tcttttgttg
    13441 ctgataagat agcccaggaa aaagggatat cttatgaaga agtatgtaga gttacaaaag
    13501 aaaatgcaaa gaaatttttt aatataaaat agacaaatta aatcttataa tgatatatat
    13561 aataagtcat tataagtata ggatgagagt gcctcaaaat gagtttttta gttcatgagg
    13621 cactcttttt tgtatgaaat caaatatatt ttcatcattt caacaatgaa aaagaagttt
    13681 atcatatttt gagatagttt gtttttattt atttaattca tattttaaaa tatattttct
    13741 taacattgtt aaaaaagaat ttacaagaga ttcattagaa ttaactcaac tctaatattc
    13801 acttaactta agttgtttat tatattatta aagagaagta aacgaaaggt agattaagga
    13861 ggttgaattt tgtatacagt tgatagtact aagatattga taaatatgaa cttagagaaa
    13921 ctagaaaaga tatactcaat gataaaagat acatcaagta tttctataat taaagaacct
    13981 aacttggcta cagtaatggt aagagctaat gaaagtgtaa aaaacacaac atttaattta
    14041 ggagagatat tagttactga atgtagtgta aaggtagatg aaagcttggg ttacggaata
    14101 gtttcagaaa acaataataa gaaagccatt tatttagcag ttatagatgc agttttacat
    14161 agcaataaca gtaaatttga tgagttaaag aattacataa ataaaagtgt atatgaagag
    14221 aacttaaggt atgaacaaga gattattgat gaatttagct tgattaataa aacgaaagtt
    14281 caattcaatg ctatggatta aataggagga gaatcatggt aaattataca caagaaatat
    14341 atagaaagat gctagatagt gcttcatttc ctgggaaatt aaataatata gataatataa
    14401 atatagaaaa taatacaaat ctattgaatg gagcaattgg tatagcaatc actttattag
    14461 accaagaagt taccttctat atagaaaatt atactcaaaa agatataaaa aatataagag
    14521 cacttacaat gtcaaatcaa atagattata gaaaatcaga ttatatattt ttagatatta
    14581 attcagaagt ggatatatta cagattaaaa taggttcttt tgaatatcct gatgaaagtg
    14641 caactatagt acatcaagta aatgatttat caacgtactc tcaaactaag tatataaagc
    14701 ttgatttgag tggtcctggt ataaaatcta aaaatagtat atatataaat ggaattagag
    14761 aagaatttat agaaagatta tctagtataa ataaagatta tccaatagga atagatttga
    14821 ttttagtaga taaaaaagga gaaattgcat ttataccaag gtcatcaaaa ttaagttggg
    14881 aggtattata gaaatggctt atgtagcagt taaaggtgga gaagaagcca tattacaggc
    14941 taaaaatata cttgagtttt atagggtaaa aggagatagc ttgcctatag atgtaaagca
    15001 gattgaggaa caaatgtctt atgctgtaga caaagtaatg tctgaaggaa gtttatacaa
    15061 caaggaacta tcagcattgg ctataaaaca agctcaaggt gatatattag aagcctcttt
    15121 ctatgtaaga gctttaaggt caacaatgcc tagaattaca tattcagaac ccataaatac
    15181 agaagaaatg agaataaaaa gaagaatatc agctactttt aaagatatac caggaggtca
    15241 gtatcttggg gcaactagag actatgaaaa tagaatattg aatgtagaat tattagaaga
    15301 agatgaaaaa aagaatagac attttagaga tagttttata aatgaattag aaacagtaga
    15361 tgaaatccct gaatttacaa aaatatctaa tatatttaga aaagaaaatt tattacaaga
    15421 agaagtttgt gaaaacaaat tggaagaagt tattgatgat attacaagaa atagtttaag
    15481 atttccagtt tctagggcag caaagttaca ggcattaact agaggtgaaa gcggagtagt
    15541 atcaggtttt gcttatgcta ttgtaagagg ctttggtgat gaacatccat atataagtga
    15601 acttagaact ggcaatgtgt ttataaaaat aaaacatccc atctatgaag atgaagaaat
    15661 aacaataggt gatatgttgg ttactgaatg tgaaatagtt tcaaaagcat cgcaaaataa
    15721 gactgaaaat tctaaaaata attcggaaga taaaaataaa atgtctttag gatatggtct
    15781 ttgtatgggg aacaatgaga ataaggctat atctatggct atactagaca aatctttgga
    15841 gccaaatagt gaaaaactag cacaagacga agaatttgta ttgcttcata tagatggaat
    15901 agattcatta ggatttatat ctcactttaa gcttcctcac tatgtagatt ttaaagcgga
    15961 tgtagaaaga ataaaaaagt ttgaggaggt aagtttaggt gatatataac tatggttttt
    16021 tagacgaaaa cacaaaaaaa gaaataagaa gaaagatatt aaaagcaata tcaatacctg
    16081 gttatcaagt tccctttggt tcaagagagt tgcctatagc taagggatgg ggtactggag
    16141 gtttacaatt aacactatca ttaattggta aaaatgatgt agtaaaagta attgaccaag
    16201 gaagtgatga ttctacaaat gcttgtaata taaggagctt tattgcaagt gtaagtaatg
    16261 tagaaaccac aaaagataca ttagaagcta cattgattca aacgagacat agaataccag
    16321 aagaaaaact taaatctaat caaatactaa tatttcaagt accaatacca gaaaccctta
    16381 gaatagtaga acctagtgaa gtagaaacaa gaagaatgca ttcagaagaa gattatagta
    16441 ggatgtgggt atatctttat gaagatatag ttagatttaa tgatatatct atagcagttg
    16501 aatatccatg taaagtaaat gatagatatt taatgaatcc atcacctata cctagatttg
    16561 atataaaaaa attaaatatg tcagacaatt tattcttgtt tggtgcaggt agagaaaaaa
    16621 gaatttatgc tattcctcca tatacaaagg tagaaccttt agagtttgag gattataagt
    16681 ttgaagagga aaagtttgaa ggaaaatact gtagtttatg taacagtaca aatacatttt
    16741 tagatgaagt atatgacagt gacactaatg aaaagtacta tagttgctca gatacaagtt
    16801 attgtgaaaa agttagatta aaaaataaca gtatagatgt gacaatagga ggtacttgga
    16861 atgagtaaaa taaatgactc taaaagatta gaaaatatag acaagcaagt attaatagga
    16921 agaaatatca atcttattta tggagatggg tgtgaaaagt gttttgaaag cacaggatat
    16981 gaaagcaata atatatgtaa gtattgtggt tctatagttg catgtaataa tgtaaattta
    17041 gaactttatg aaggagaagt ccttggaatt gtaggtgaat ctggttcagg aaaaagcact
    17101 ttattaaaaa tactgttttt ccaagaacat gcaaatagtg gagaagtata tatatcccat
    17161 tacaataaag acacaaatat actagagctt tcagaccaaa aaaagagata cataaagaat
    17221 cattttatgg gtattgtgta tcaaaatccc caccttggtt taaatttaga ctttagtagt
    17281 ggtggtaata tagcagaaaa attattgatg gcaaatttat ataatgttga aaaaataaga
    17341 gatagaggca aagaactttt aataaaaact aatatacctg tagaaagaat agaccataaa
    17401 ccaaaatact ttagtggggg aatgcaacaa cgagttcaaa tagcaaaagc tctttcaaat
    17461 aaccctccaa tattgctatt agatgaggtt acaacaggtt tagatgtttc tgtgcaagcg
    17521 gaagtattgg atttaataag agaaatccaa agagaattta agatttctat gatagttgta
    17581 tcccatgact ttgatgttat aaagatgctt gctgatagga caattgtaat gaaaaatgga
    17641 agtgtaattg agtctggtct tacagaccaa ataatggagg acccacaaca cccatatact
    17701 cagcaactta taaattcatt gctataaata tcgtttgtaa ataagcaaaa tgggggtttt
    17761 taataaaatg aaagaaacta tacttgaagt aaataatttt agtaaagact ttgagttaca
    17821 tgcattgaac aagactataa cggcatgtag taatataagt tttgaagttt caaaaggtga
    17881 gtttttagga ataataggta aatctggagc aggtaagtct accatattaa aaagtatcta
    17941 taaaacatat ataccaacaa ctggagaaat aatttttaat tcagaaatat atggtaatgt
    18001 agacctggct aaaattggag accgagaaat gataaatctt agaaagaaag aaataggata
    18061 tgtatcacag tttttaaaga cacttccacg aataactgca attgaactgg tagtacattc
    18121 tttaattgag agtgggtttg aaaaggattg ttcgtatgat atggcaaaag atatacttaa
    18181 ccaatttgaa ataaaggaaa atttatggga tgcgtatcca aataattttt ctggtggaga
    18241 aaaattaaga ttaaatttag ctcaagctat ggttaaaaag cctagacttt tgttacttga
    18301 tgaaccaact gcatcacttg ataatcaatc taaaatatat gtaaaagaaa aaatgttgga
    18361 gcttaaaagt caaggaacta caatgatagg aatttttcat gatatagagt ttatggaaac
    18421 tgttatagat aagaccttta ctatgacaaa agggactata agtgaaagtg gggtagccta
    18481 gatgataaaa ctaattcata atgcaaatat tgtattagaa gatatgattt tagaaaatgg
    18541 tcatttaatc atagagggag atacaataaa aaaagtatca aatgataaga ttgattacgc
    18601 atttgacttt aatgagatag atgaaataat agatgcaaaa aacttatatg taatacctgg
    18661 tattatagat attcacagtg atgccattga aaaggaaata gagccgagac caagtacatt
    18721 attaccattt aatatggcat tttatgaact agataaaaaa ctacctgcaa atggaataac
    18781 tacagtttat cattctatat ctttagggga tggagtagga gttagaagta ttgataactc
    18841 tttaaagatg attaaaaata ttgattcata taaaaatata gatagtaaga gcataaatca
    18901 taaagttcat ttaagatatg aagtgttgta ttatgaaggt ttagaaaagg ttttagaact
    18961 attagatgaa aataaaatag actacttatc aataatggac cattctccag gacaaggtca
    19021 gtatacaaat ccaacatttt ataaagagta tgctactaaa gtgtggggag ttacagaaaa
    19081 ttatgtagat acatggttag atgatttagt aaatctgcat gataatttag attggaataa
    19141 aatagctaat attataggta tagccaaaac taaaaatata aatgttgcat cacatgatga
    19201 tgatacatta gaaaaaatgg atttcataaa aaatataggc ataaatgtat ctgaatttcc
    19261 tataactctt gaagttgcaa aacattcaaa gaaattgggt gttctaactt gtttaggtgc
    19321 accgaatatt gtaagaggta aatctcataa taacaattta aaagcaatgg atgctatatt
    19381 ggaagattgt tgtgacattg tgtgttcgga ttatcttcca tcagctatga taaagtctat
    19441 gtgtatagtt gcagaaagaa taaataattt aaataaagca gtcagtttat ttacctcaaa
    19501 tcctgctaaa gcagttggta tatatgatga aagaggtagt ataaaagaaa ataaaaaagc
    19561 agatttagtt ttagtagata ttgatagtga atatccaaag gttgtaaata ctattgtaaa
    19621 tggaaaaaca gtttataaaa gggaggtata aagattgcta acagagttac attgtcatac
    19681 aagtatatca gataataatt ttactactga agaaataata aaaaaagcag cagaaaagaa
    19741 tataaatatc ttagcaataa caaatcatga cacattatct ggattggatg aagctcttgt
    19801 gcttggagag cattatggaa taacaataat accaggtata gaaatttcag cctatgatta
    19861 taaaaataga aaaagagttc atattcttgg atataacata gatttaaaca gtcaagaaat
    19921 aaaaaatttg tgcaatccaa tggtatgtga cagacataaa gcatctgtta agatggtaaa
    19981 taaaattata gatttaggat acaaaatatc tttggaagat gtaaagaaat attcatcaaa
    20041 gactggaata tttaaacaac atataatgaa agctcttata gataaaggat atacagataa
    20101 tatatattca agtctatata aagaattgtt tcatagagga aacggcaagg tatatgtatc
    20161 tttaaaatat gtggattaca gagatgcaat aaaagctata aaaaattctg gtggaatttg
    20221 tgtgttagct cacccgggtc aaatggataa ttttagcgct attgaagaga tggtaaaagt
    20281 aggattagat ggaatagaag tatatcatcc atctcacaac aaagaactag aaaaagagtc
    20341 gctttcttat gctaagaagt ataatctagt ggtcacagga ggctcagatt accacggatt
    20401 ttattctcaa cataattatg aacttggaag caagagctta aataaagaag atttatttaa
    20461 gtttaggtta gctttaggag gggtattata aatgtctttt ttacaagtaa aaaatgtagg
    20521 taaaagttat ggacaagtta aggtgttaaa agacatatct attgatattg aaaaaggaga
    20581 gtttatttgt ttactaggtc caagtggttg tggaaaaagt acactgctta gaataattgc
    20641 agggcttgag gataaacatg gtggaaaaat aataataaat gataaggata tgactaattc
    20701 accacctgaa agtagaaatt ttggtatagt ttttcaatca tatgcattat ttccaaatat
    20761 gaatgtttac aaaaacatag cctttggact agaaaataaa aacataagta aatctaatat
    20821 tgataaaaaa gttaaagaag ttctagaagt tgtagaatta agtggatatg agaaaaaata
    20881 tccatcacaa ttatcaggtg gacaacaaca aagggttgca ttagctcgtg cgattgcttt
    20941 agagccagac tttttacttc ttgatgaacc tttatctgca ttagatgcaa aagtaagact
    21001 taaacttagg gagcagatta gaagtttgca tagaaaacta ggcattacaa ctataatggt
    21061 aacgcatgac caagaagaag ctctttgttt agctgataag atggttgtta tgaatagagg
    21121 agaaataata caagttggaa cgccaaagga ggtatataaa aatccagaaa caccttttgt
    21181 agctgatttt ataggaacaa taaattttat agatgatggt attaataaga tagctattag
    21241 accagaagat ataaaagtag agtctaatag agattctaaa gataaggata taaaagtggg
    21301 agaaatttta gatatcgaat ttagaggatt taactataga attacagttg agtatcgttc
    21361 taagcagatg aaattagatg tagtttccaa agttgcagaa caaatgaaac tgtgtatagg
    21421 ttctaaaata aactttaaga tacctaaaga aggaattgta caatataaat cagagggatg
    21481 tgcttaatag ctaggggtga ataaatgata acatcaaaaa ataaaagaag aataagtgat
    21541 agcacaatac aaaaatattt cttgattttt tcggtaattg tactaatagt atttatatta
    21601 tttccattag tatcattatt aaaaaatgca tttatggata caagtggaaa ttttatagga
    21661 atttcaaact ttagtaaata tgttgaaaat ccaagtctta tagcatcatt taaaaattca
    21721 atttttgtat ctacagtctc atctttaata tcactatgtt tagcatttat atattcatac
    21781 gcaataacaa gaacaaacat aaagtttaaa gaatttttta aaactatggg aatgtttcca
    21841 ctttttgcac caactatgtt atatggtatt tcacttatat acctttttgg aaataaggga
    21901 atctttacaa ccatgggtct ggaagtacca ctatatggtc cattaggaat aattatttct
    21961 cagattctat ttacatttcc acaagctttt ttaatattat ctgtagcact ttcgatggct
    22021 gattacagac tttatgaagc agcagatagc ttgggtgcta gtgaatttaa gaaattttgc
    22081 aaaataacaa ttcctggtat aaagtactca ttacttagtt cattctttgt atcctttata
    22141 cttgcattta cagattttgg agcagctaag gtagttggag gaaactacaa tgtactggct
    22201 actgacatat ataaacaagt agttggacaa tttaacatac caatgggagc tactgtttct
    22261 atggtaatgt taattcctgt actaatagca tttacattag ataagatggc aagtaagaaa
    22321 caaggtatga ccataactgc aaagtctatg ccatataaga ttaaggaaaa taagtttaga
    22381 gatagagtat ttttaatagt atgtagtttt ataacattgc taatttttat tttaatattc
    22441 gtttcagtta taggttcagt tataaagtta tggccatata atttaagctt tacaatggaa
    22501 cactatacat ttaaaggtat tttgggaagt ggaataaaaa catatttaag cagtttgaaa
    22561 atatcaactt taacagcaat atttggaact atatttgtat tttttagtgc ttatatgata
    22621 gaaaaggtgg ataagtttcc taaattaaga caaacagctt accttttatc aatgatacca
    22681 atggccttac ctggcttagt tttaggtata tcatatataa ctttttttaa ctcagcaagt
    22741 aatccattaa attttttata tggaagtaca gcaatattag ttatagtaaa tgtagttcat
    22801 ttttattcag tttgttttat aacctcaaat tcatctttaa aaatgttgga taaagagtat
    22861 gaattagttg caaaatccat aaatattcca ttctataaag tattttttaa tataacagtt
    22921 ccaatgtcta taacatcaat acttgaaata gtagtatatt attttgtaaa ttcaatggtt
    22981 actgtttcag cattaatatt tttatataca cctcaaactc agactgcatc aatatcaata
    23041 ctaaagttag atgagatagg atatatagga ccatcagctg ctatggcagt attagtgctt
    23101 ttaacaaata taatagtaag acttttgtac gaatttgtaa caaagaaatt aaaaaataac
    23161 acacaaaaat ggcagcaaaa agatgttgca taggggaggt aaaaatgaaa tttaagaagc
    23221 tagtttcttt aggagtatta tcaacacttt tattaagtgt aataacagga tgtagtactg
    23281 attcatcagg ttcagattca aagtctgata gtaagacaaa aggggattta acagtttaca
    23341 cagcaattga agaagatagt atagagccat atttagctac atttaaagag aaatatccag
    23401 atattaaatt aaatattgtt agggcatcaa caggagatat aacagcaaga ttattagcag
    23461 aaaaggataa tcctcaagca gatgttgtat ggggagttgc agccacaagc ttattggttg
    23521 cagatgacca ggggatgtta gaaccatatg ctccaaaagg ttctgaagaa atagagtctg
    23581 catttaaaga tgataaagaa gttccttctt gggttgggat agatgcttgg atgacaggta
    23641 taacagtaaa cactaaagaa ttaagtgata agaacatccc agttcctagt tcttacgaag
    23701 atttaataaa gccagaatat aaaggtctta tatctatgcc taatccatct tcttctggta
    23761 cagggtactt aacagtatca gcccttatac aaattatggg ggaagaaaaa gcatggcagt
    23821 atatggataa gttacatgag aatattggtg tatacactca atctggttca gctccagcta
    23881 catcagcagc ttcaggagaa tatcctattg gtatatcttt tggatataga ggtataaagt
    23941 taaaagagga aggctatcca gtagaagtag tatttccaaa agaaggttct ggatgggata
    24001 tagaagcaaa tgctcttgtg aaaaaagata atataaaaga agcatcaaaa gtatttttag
    24061 attgggctat aagtaaagat gcaatgaacg aatactctaa aaactatgca gttactacta
    24121 tttctacagg aaatccaata ccagaaggat tccctaaaaa gccattagag caaatgatag
    24181 ataatgattt aaaatcagca gcaaaaaata gagaagatat actaaataaa tggatatcaa
    24241 aatatgatgg taagacagaa aaagagagtt aagtaaaact atacaaatgg agtaaaaaga
    24301 aaaatttttt ttaagtagat tttattatta taatataagt tgagaataat ataggtcaag
    24361 aaaagtgaat attctataaa aatgtataga gtaagtatac cattcagttt gaatggtata
    24421 ttttttattt ttagagatta atttagaaat agatatacta cagtttaaaa tagtatattt
    24481 taatttcatt attaaagtat agatgttata tataaagtaa ataagatatt aaataattat
    24541 aaactaaagt aaataaaata ttgagcgatt attaaaaaga tatatatata atttaatttg
    24601 agcagtttgt tatatattat aaaaaaagag aaaaataaaa ggtaaattgt ttctggataa
    24661 cttaaaattg gaagtaagaa tatttaacta gagaagaatt atttaagttt aggttagctc
    24721 taggagtagc tgattttata ggaacaatag attttatgga tgatgatata aacatggtag
    24781 caattagacc agaagatata agggtagaat ttaatagaga tttcaaagaa attatagacc
    24841 tcaaactcag accgtatcga tataaaaact aaagttagat gatatagtgt tgtagatagg
    24901 accatcagct gttatggcag tattagtgct tttaacaaat ataatagtaa aacttttgta
    24961 tgaattgata ataaaaaaat taaaaaccaa gacataaaaa tggcaacaaa aagaagttga
    25021 ataagggagg taaaaatgaa atttaagaag ttagtctctt tgggaatatt atcaacactt
    25081 ttattgagtg taataactgg atgtagtaat gtttcatcag gttcaaactt aaagtctaat
    25141 aataagacaa aaggagagtt aacagtttat acagcaattg aggaagatag tatagataca
    25201 tacttagcta catttaaaga gaaatatcca gatattaaat tgaatgtact tagattatca
    25261 gtaggaggta taacagcaag attattagca gaaaaagata atcctaaagc agatgtgtta
    25321 tggggtgttg cagctacaag tttgataatt ttagatgaca aagaaatgct agagccatac
    25381 gctccgaaag gctttgataa ggtaaattct gcatttaaag atgataagaa agttccttct
    25441 tgggttggaa tagatgcttg gatgactggt ataacagtaa atactaaaga attaaaagct
    25501 aaaaatatat cagctcctag ttcttatgaa gatttaataa agccagaata taaagatctt
    25561 atatctatgc ctagtccagc ttcttcagga acaggatatt taacagtatc agaactcata
    25621 caaattatgg gtgaagaaaa agcatggcag tatatggata aattgcataa aaatattggt
    25681 gtatacactc agtctggttc agctccagct atattagcag cttcaggaga atatcccatt
    25741 ggtatatctt ttggaaatag aggtataaag ttaaaaggtg aaggttatcc agtagaagta
    25801 gtatttccaa aagaaggttc tggatgggag atagaatcaa atgcacttgt gaaaaaagat
    25861 aatataaaag aagcatcaaa agtattttta gattgggcta taagtaaaga tgcaatgaat
    25921 gagtattcta agaattttgc agttactaca attgacacgg gaaatccggt gccagaagga
    25981 tttccaaaga atccattaga acagatgata gataatgatt taaaatcagc agcaagaaat
    26041 agagaggcta tactaaataa atggatagaa aaatatgatg gaaagacaga aaaagagagc
    26101 taagccttgc aataaaattt agtacaaaaa gcgcctagaa tataagtttt atatctaggc
    26161 tcttttgata tatttattta aaatgcatat ttctatataa aaataaatag tttagtacct
    26221 tgattaaatt aaataaaaac cttaaaataa agagatgtaa agaaaaacta aaaacgaata
    26281 aaaggtgaat ttatatgata aaagaaataa tagtagtaga gggtagagat gacgttacag
    26341 cagttaagag agcaatagat gcagaactta taactacagg tggatttgga tttccaaaag
    26401 gtgtaatgga gcgtataaaa gctgctaatg aaagacgtgg tgttataata tttacagacc
    26461 cagattttgc aggtgaaaaa ataagaaaaa aaatagcatc agaagttcca ggatgtaaac
    26521 atgcatttct accgagagag gaagctaaaa aggatggaga tataggtatt gagaatgcta
    26581 ctcctcaaag tataatagca gctctgaata aagttagaac tgaaaataca gaaaaaagag
    26641 atgaatttaa gcaagttgac ttaattagaa atggtcttat aggtaatgaa gaagcatcac
    26701 ttagaagaga tgcacttgga aaaatacttg gaattggata tggaaatgca aaacaatttt
    26761 taaatagatt gaataattat ggagttgaaa gagaagaatt tatagatgca ttaaaacaaa
    26821 tctaaacgta atgtttaatg tatgaaaaat atgtatataa aataaattgt ggaattacgg
    26881 ttaatttaag atgaattatt tataatatac accttatttt aaatcaatgt aaatacatat
    26941 aaataagtat aattaataaa attaaatatc aaaataagtt aaaaaggagt aaataatgga
    27001 tagactatct tcacataatg caacaaagga agtagtacaa aaatataatt ttaaattttc
    27061 aaagtcttta ggtcaaaatt tcttgataga tagtaatata attgataaaa tcttatctgg
    27121 agctagaatc acaagaggag ataatatcat agaagtagga cctggtatag gaactcttac
    27181 tcgtgagatg ggtaaaatag cagaaaaagt agtggctata gaaatcgata gaaatttaat
    27241 tcctatattg aaagatacct tgtcagattt agataataca gaggttgtaa atcaagatat
    27301 actaaaagta gacatacagg aattagtaaa agataaatta aatggtgggc cagttaagct
    27361 tgtagcaaat cttccatatt atataactac tccaatagtt atgaaatttt tagaggaaga
    27421 catacctgta acagacatag tagtcatggt tcaaaaagaa gttgcagata gaatgaatgc
    27481 tattccgggt actaaagatt atggggcatt atctatagct gttcaatact attgtgatac
    27541 agagattgtg gcaaaagctc caagacatat gtttatacca caaccaaatg tagattccac
    27601 agttataggt ctacatgtaa gagataaaag aaaatatgat gttcataatg aagacatatt
    27661 ttttaaaaca gtaaaagcat cctttggaca aagaagaaaa actcttctta attcgttggg
    27721 aggtcttggt tttcttaata aagatgaaat aagagaagta ttaaaagaag ctaatataga
    27781 cgaaaaaaga aggggtgaaa ctcttagtat agaagagttt tcagttcttt caaatataat
    27841 aaatacaaaa gtatcttcta aatagaaggt acttttttta attttgttaa catatattgt
    27901 ttagagaaac tgtgttaagg ggggattgca gtgggttcta aaagaaagtt taaaagagac
    27961 tatataatac tagaagctaa agacataaac tttagataca aagaacgagt actgccaaaa
    28021 gcttttgcaa aagttgaagt aaatgatgaa aaagcaatta ttgcattata tgtagaaaat
    28081 ctaaaatatg ttaaagatgg gtataaagca gtagcaataa aagatgacca tggattaata
    28141 gacttaggaa atataatttt aaatgaacaa ggaagaggtg aatttgtttt agatttagaa
    28201 gattatgatg atgagataaa aggtattgca ttattgtatg aaagaaatat acctttaatt
    28261 ggatttaaag gaaataagct tgaaaattat gaggacatat tatttcaaga ggtagaggaa
    28321 gaagaatatg aagaatatga agagttggaa gaagaagacg aagagataga ggagaatgag
    28381 gaaagtgata gagacgaaga tagtgaagaa gaatatatta tagaagaata tgatgaagat
    28441 gagtatgaag actattggga agatgatgaa gatattgaag aaggagagtt aaaagcaaga
    28501 acggaagtag aagattctga tgatgaggaa atagaagagt tcaataatta taaggaaata
    28561 gaaaattata atagggttaa agtacaggaa gaatatatag acatagaata tgatgaagaa
    28621 gaagaggaag aagaagtttt tgaagtaaat agagaagaag ataggtttgt ttcctcaaaa
    28681 cctactaaat caaagaaagt tcaaaaagat cagaataata ttgaaaaaat aaaaagagct
    28741 aatggagtaa gtcaaggaga gagtggaaat aaacaaacca gtacattact tatgccaaga
    28801 caaataaaaa aaggtttgaa attatttaaa gaagtaaaac cttttgtaac agattatata
    28861 gataagacaa gatggtggaa aatagagata aatcctacaa cactgtgtgg atatacaatg
    28921 ccttatttag gatatttaaa cgtattgaat tacactatgt atagtgatgc agttttacat
    28981 tcatataaat acagacacta tttatttgga gttcagtatg atgaatataa taagagaaaa
    29041 cattatattt atgctatccc aggaaataga aatgaacagc cagacaatgg aagcacagga
    29101 tttttaagat atcaagcttg tgataataga acaaatagtt taggatattg gctatgtttt
    29161 atagattcta gacaaagaaa tatagttagg taaaaataat aaaaatgaaa aaatttagac
    29221 tcaattctta ttttactgaa aaaattaatg aaatgtagta caaaacttta aaaactattg
    29281 ataatattta aatattacac tacaatatat cttgaaatac ctattatatt atggaggaaa
    29341 attatgataa acgagcaaag aattttaaat gagtttcttg aattagtaca gatagatagt
    29401 ttatctttaa aagaggggaa tgttgctaaa gtattagtta aaaaattaga agaaataggt
    29461 tgttcagtag taatagacaa tgctggtgaa aaagcaaatg gagaaactgg taatataata
    29521 gctacattaa aaggaaataa agaaggaaag aaaatattat ttagttcaca tatggataca
    29581 gtaactccag gtataggggt aaaacctata gtggatgaag caaatggaat aataaaaagt
    29641 gatggtacta caatattagg ttctgatgat aaagcaggta tagcagctat attagaaggc
    29701 ttaagatata taaatgaaaa taacatagaa catactgata tacaagttgt attctctata
    29761 tgtgaggaat gtggattagt tggagctaaa aatttagact atggaaaaat agattctgaa
    29821 tatgcattta tattagatag tggtggttct ccaggtgaaa taatagtaaa agctcctgca
    29881 caagatgtaa taaatgttaa gatattagga aagactgctc atgcaggtct tgaacctgaa
    29941 gctggaataa gtgctataat ggtagctgcc agagcaatag aaaatatgaa tttacttaga
    30001 atagacgaag aaacaactgc taacatagga attataaacg gtggaacagc tacaaatata
    30061 gtaacaggag aagttaatat agttgcagaa gccagaagtc taaaagaaaa taaattagat
    30121 gttcaaacta aacacatggt agagactttt gaaaaagctg cgaaagattt tggagctcaa
    30181 attgagatag atgttaatag agcatacaca ccaatagatg ttagtgagga ttctgaaata
    30241 ataaaacttg caaagaaggc tttttcaaac ttaggtatag aaggtcatac tgaatctaca
    30301 ggaggaggta gtgatacaaa tatattaagt aagaatggta tcgaagctat aactttagga
    30361 attggtatga aaaatgctca tacactaagt gaacatatag ctataaaaga cttatatgat
    30421 tctgctaaaa tggtagtgga gataataaag gaagcataaa tttaaataat agtgtataat
    30481 atatatatcg cctagcttaa gctaggtgat ttttcatttt atagaagatt gataatctct
    30541 attgaaagca aagagtatac atttgcatat aagagagtgt ttaatttatg ttcaaatgta
    30601 ggttcttaat aatatttaga ttgtttcata agggaggaat aaatatggaa acaaggtatg
    30661 aagaagcaaa taaaataaca atacaatcaa tactttggaa tatagtatta acaataatta
    30721 aggttatagc tggtgttatt ggtaattcta gcgctatgat tgcggatgga ttacattcag
    30781 catcagatat tataagttct ataggagtac taataggcaa ttatgtatct tctagacctg
    30841 gagatagaga gcataattat ggtcatgaga aagcagagac attagtatca tttgtgttat
    30901 caatattgct tattttcgtt tctataacaa taggtataga ggcaattaaa tctttattta
    30961 acttagatgc attaagtgtt ccatcaatat taccattagt agtatcagtt atttcaatcc
    31021 tgataaaaga gtatcaatat agaataacaa taaaagttgc taaaaagata aattcaccag
    31081 ccctaaaagc tgatgcttgg catcacaggt cagatgcttt atcttcagta gctgctttta
    31141 taggtatagg tggttctatt ttagggttta aaccattaga cccaattgcg tcagttgtag
    31201 tggctatatt tgtagctaaa gtaggaatta gtatacttat aagttctgtt aatgaattga
    31261 tggatgtttc agttgatgaa gaagagataa aagaattaaa atttattgta gcagatacag
    31321 aaggtgttaa aaatcttggt gatataaaga ctagaaaaca tggagctatg gcatatgtag
    31381 atttgactat atgtgtggat gaaaatttga cagttaagca aggtcatgat attgctacca
    31441 agcttgaaaa acatataata aaacatatgg aatttgtaaa aggtataaca gttcacgttg
    31501 aaccatgtac taattgccaa ggaaataagt gtaataatta attaaaactt aaaaaaaagt
    31561 gtaaaaagtt atataattgt tttatgatta taaactctag gggtggttaa atgagcaaga
    31621 aaagaaaact aaagaaaaaa gctgcaatat taataggagt agttgttttc tttataatat
    31681 actttatatc ctttagaata ggaataaata tggaaaagga taaaagtgaa gtgccaaaga
    31741 aggtagaaaa agaagaagaa gataataggt ctttaaggac tcaaataaaa gagaagaaga
    31801 aggtttatat atcagataaa aatatagaaa acataaaaat tgaagaagat atgtgggatg
    31861 aaataaagtt atttttctca gattttaaga aagtaagaaa tactacttct tcttacgaag
    31921 cagtttatga aggttattct gatgatggag ttaaattttc tactgacctt aactttttta
    31981 gagtatatac tgtaaataaa gaagaatatt ataaagtacc agttagttct aaggatgctt
    32041 ttaagaaact tttagatgag agtatatata cttcgtttga ccttttaagt cattataaag
    32101 attggaaaga ggttactata actcatggaa aagaaattaa aaaagttcat agatggaaat
    32161 ttgatgattt agcacataag atgatttcaa agagggttgt aggaaaagtt caacctgaga
    32221 aaagtaagga aagaagtgac tataatttta ctataaatat aaaaggtgat aattttgatg
    32281 taaaagttga tacaatggga aaagattatg taaaagttac ttctaagaaa aatgtatctt
    32341 actatgaggt tcatacaagt ttatttgatt accttaaaaa tgagatattt gaaattggaa
    32401 agaaataaga ctttaagaag gctatcaaat tagatatttg atagcctaaa ttattttata
    32461 tattattttt caaccaacaa gtctgcaacc ttataaattg gaccagcacc agcagttatt
    32521 actaaatcat tatccttaac attttcacgt aagtatttta ctatatcttc aaattctttt
    32581 atatagatgg catctacatt gttttgatat aatttctcaa ccaagtcctt agaatgtata
    32641 tctcctgggt ctttttccct agcagcatat atatctgtta tgataacttt atcagcagca
    32701 tagaatgcct ctgaaaattc acctaaaaga gattttgttc tagtataagt atgtggctgg
    32761 aagatacacc ataaagttga tttttttaat tttttggcag cagctaaagt tgcttttaac
    32821 tcagttggat gatgtgcata atcatctact actaaagcat tcttatagta ccctttagtt
    32881 tcaaatcttc ttccgacacc tttataaatt tttatatttt ttcttatagt ctctaagtca
    32941 ataccagaaa caagagctgc cattatggca gaagaagcat tgtatatgtt atgaagacca
    33001 tatacagaaa gttcaaattc tcctaaatct cttccttcat attctattct aaatattcca
    33061 tgaccactat tgtcaaagtg aatatctttt attacagcat cattgtcaga atctctgccg
    33121 tattttataa tagtagcttt aacatcatac aatatgtcat caatattttc atcatcgcca
    33181 tttattatga aatagccttc tgggggtaat aattttccaa atttgttaaa agaagccttg
    33241 atttcatcta ttccagagaa ataatcaaga tggtcttctt caacatttaa aactatagat
    33301 atctttgggt taaaattaag gaaactatct acatattcac atgcttcagt tataaagtgg
    33361 ttggaattac ctatcttaac attaccacct atcatactta agttaccacc aactagtata
    33421 gttgggtcta agtctgcata ttcaaagatt gtagataaca tagatgttgt agaagttttt
    33481 ccatgtgttc cagaaactgc tatagaattt ttatattccc tcatgatttg gcctaaaaaa
    33541 gttgctctgt tcattaccaa tttatttttt tctttagcag ccacaagttc ttcattgtct
    33601 ggatgaactg ctgctgtata aacaaccata tttacatcat ctgatatatt atcttttttc
    33661 tgaccaatat aaatagttgc cccttggtcc ctaagtttgt ctaataggta tgattcgttt
    33721 gaatccgaac ctgaaacttg ataaccttta ttgatacata tttcagctaa tgcactcatg
    33781 ctgataccgc caattcctat aaagtgtata ttcataataa aaccaccttt ctttttaatt
    33841 aagtcctaca atatgaacta tttctgtggt ataataatat aatgttcaat attttataat
    33901 taatatgcat ataatattat atgtatttta tttttgaata taataaaaca taagtaatat
    33961 tataccacaa taagaattgt tatgcattag ttagtttaac gttatataaa ggtacttgta
    34021 ggaggaaata ataatgaaat ttaaaagaac agagagaatt ggagcaatag ttaaaatact
    34081 gtctgataat ccaaataaaa tatatacttt aagttatttt acaaatcaat ttaatgcagc
    34141 aaaatcaaca ataagtgaag atttattagt tgtaaaaaat gtatttgaga agctacattt
    34201 aggtaaagtt ataactattt caggagcagc aggaggagta aaatatatac ctaaaacttc
    34261 tatagcagaa aatgcagagt tcttaatgga attatgcgag agaatatgtg acaagtctag
    34321 aatactatct ggtggttttt tatacttgat tgacctaatt tacgacccta caatagctgc
    34381 taagatagga aagatattcg catcaaatat tgagtatgta gatgcagatt atgttgtgac
    34441 aatggaaaca aaaggtatac ctatggcact tatgactgca aaagctatga atttacctct
    34501 agttataata agaaaagata taaaggtatc agaaggtcct acacttagca tgacatatgt
    34561 gagtggaaac agttctaaag ttgaaagtat gagtttgcca agaaaagctc ttaaaccaga
    34621 tagtaaagtt ataattatag atgatttcat gaggggtgga ggtactataa aaggaatggt
    34681 agaccttatg aatgaatttg gtgcagaggt tataggtact ggagtattca tatctacaac
    34741 gaatccttca gaaaaaatgg taaaagatta tatttcattg attcaattgg atgttaatgg
    34801 agataaaata gtagtagaac caaacttaaa gacttttaaa gatgaatata gaaatgaaga
    34861 tttagacgaa gaagaagatt cagaatttga atttgagata gatgaagatt aataaaaaaa
    34921 atatttagaa aaatatgtaa aaataaaaag aggatatccc tagttgttca tagaattatt
    34981 taaaaaacag tttatattaa tttattccag cgataataat tttaatataa attaatttct
    35041 gtcaaaatca aagggggata ttttggtatg aagataactg acgtaagagt aagaaaatta
    35101 acagaagaag gtaagatgaa atgtatagtt tcaataactt tcgataattt gtttgtagtg
    35161 catgatataa aagttataga aggccataat ggactattta tagcaatgcc tagcagaaaa
    35221 gtaggcgaag gaaatttcag agatatagct catccaataa atgcagaaat gagacaagtt
    35281 ttagaagatg cagttttaca agcgtatcat gaagcattag ttcaatggga agtagctgct
    35341 gaataataat aaatatataa aatggagaga gcccgaacat ttaggctctt tttaaatgta
    35401 gaatttctat taaaatatgt actttttagt aaaaaagtgc tatattatta gtgataatgt
    35461 atatgttgta tacaaattgt tagataaatg tattataatg tatattgatt aagagaataa
    35521 tacgaataaa attaaattag attgaatgga gtgtatcaat gaactttaaa gctataatcc
    35581 ttgctgctgg caaaggaaca agaatgaagt ctaaataccc aaaagttata cataaagtat
    35641 gtggaaaaga aatggttaac catattatag atgtttcaaa aaaatcaggg gtaaaagata
    35701 ctgtcgtgat tttaggacat gaagctgatg tagttaaaga aaagcttgca gaagaaatta
    35761 taatagccat gcaaacagaa caacttggga caggacatgc tgtaaagatg gctaaagaat
    35821 atattaatga tgaagataca atagttgtac tttgtggaga tactccactt ataaaagaag
    35881 agacactaaa gagactattt gaatatcata tagaaaataa atatcatgct acagttctta
    35941 caacaagagt tggaaatcca acaggttatg gtagaataat aagagataag aagggtgacc
    36001 ttcttaaaat agtggaacaa aaagatgcaa attcagaaga aaagatgata agtgagataa
    36061 actctggaat ttattgtttt aatggaaaga gtcttagaga agcactagat ttgctaaata
    36121 ataacaattc acaaggtgaa tattatttaa ctgatactgc taaaataatg agagataaag
    36181 gattaaaagt tggtgcattt gcaggttcaa ctattgaaga acttatgggc gttaattcaa
    36241 gagtagaatt atcaaaggca gaagaaatta tgagacgaag aataaatgaa tctcatatgg
    36301 taaatggagt aactataata gacactaact ctacctatat agaatctgat gttatgatag
    36361 gcaatgatac tataatatat ccaggtgtta tgttacaagg aaaaacaagg ataggttcag
    36421 attgtataat tggaatgaat tcatctataa caaattcaga aataggtgat ggtacagaaa
    36481 taaaaaattc aactataatt gatagtaagg ttggagaaaa ttctactgtt gggccatatg
    36541 catacttaag accaaagagt gacttaggaa ataatgtaaa aataggcgat tttgtagaag
    36601 taaaaaatgc tattatagaa gatggctcaa aagcatctca cctttcttat ataggagatg
    36661 cacatgtcgg taaaaatgta aatattggat gtggagtagt atttgtaaac tatgatggta
    36721 aaaataaatt taaatcaata gtaaaagaca atgcgtttat aggttcaaat tctaatttag
    36781 ttgcaccagt agttgtagaa gaaaaaggat atatagcaac aggttcaaca ataacacatg
    36841 atgtcccaga tggtgctttg gcaatagcaa gagaaagaca agttatcaaa gaaggatggg
    36901 tagaaaaaaa gaatcaaaaa gacgaccaaa gtaagtaatt atgtagttta ttattttggc
    36961 taaaaaattt agctcgaaat tattataaat tatattcatt agtattttaa aattttcagg
    37021 agggttacat aaatgaatac tagcggaagc gagattaaaa ttattgcagg taattcatcg
    37081 aaagagttag cacaaaaaat agctgactat ataggtgtat cagtactaga ttgtgaagta
    37141 ggtacattta gtgatggtga aatatgtgta aacatgaatg agacagtaag aggttgtgat
    37201 gtgtttgtag tgcaatctac taacagccca gtaaatgata acttaatgga gttattaatc
    37261 ttaattgatg cgttaaagag agcatcagca ggtagaataa cagcagttat tccttactat
    37321 ggatatgcaa gacaagacag aaaggctaag gcaagagatc caatcactgc taaattagtt
    37381 gcaaatttaa taactgctgc tggagcagat agagtattaa caatggattt acatgcagct
    37441 caaatacaag gatattttga tataccatta gaccacttac taggtggaac aatattagca
    37501 aattatttta atgaaaagaa aatagaagat ttagtagtag tatctcctga cttaggaagt
    37561 gttactagat caagaaaatt tgctaatact ttaaatggag aagttccaat agctataata
    37621 gataaaagaa gaccgaaagc taatgtatgt gaagttatga acttaatagg tgatgttaaa
    37681 ggtaaaaatg ttatactatt agacgatatg atagatacag caggaacaat agttaatgct
    37741 gcaaatgcac ttaaagagtt tggagcaaaa gatgtttatg cttgttgtac tcatggtgtg
    37801 ttatcaggtc cagctattga aagaattgct aattcagaaa taagtgagtt aatagttctt
    37861 gatactatac aacttcctga agagaaaaga atagataaga taaagataaa aacagtagct
    37921 cctttatttg gagatgctat aagaatgata ttctctaatg aatcagtaag taaattattc
    37981 taatttattg tatttatgag agttgtttaa aaggatataa accttttgag caactctttt
    38041 ttgattttat acttatggaa tgttattttt ttcttgttag aaaggtatat attattgtaa
    38101 aaagagatta tagtttaaaa ttatgtatat ttaagtaaaa taaacctaat aaagggtata
    38161 ttattcaaaa tattatgaat ataaaaaata ttacaaaaaa gtattgaaaa aattcagatt
    38221 atgtagtatt atgtaaatat aaataaagaa agaggtgttt ccaatgggaa ggttagaaga
    38281 tgaaccccta ataattaaat gattcagtca caatctaaag tgaatataaa aatctattga
    38341 aagtttattt ataagaattg aaaatagtat ctattctatc tttagtgttt gtgagagggt
    38401 tctggattat ataagattac tgtaaagagt aattttgcac aataagcata tctgtaaatg
    38461 aagagtttcg tttatataat gattgtggct gaatcataac tataatagat agaatgttta
    38521 taatagatga ttttataaaa tactatattg taatagcttt tagctttcaa ataaatatta
    38581 gcaaaggatg gaaatgacaa tgataaaact aaatgataat ttataaatat ctccttacga
    38641 tatataataa ttgtagggag atgtttcttt ttttgtatag cataattttt ctagaaattc
    38701 tgtttcaata tgtaaaaagt tggtatatat aaatagtata aacagtgttg ctaaaatatg
    38761 gtagatatta tataatataa gtggatatgt atatacatta tgagttatat aatttgaatt
    38821 taataaaata gaaacgttga tttatgttct gtaatgtggg caccttggag catattgagt
    38881 tagtggtgca accggctatg aaattgtatt tatttataaa tactattatt ttcatagctt
    38941 tttttattta aaaaatggtt atatgttaga aataaatttg gttagtacgt ttaaataatt
    39001 ttacttagat ttttaaccag atatattgta agtaagtatt aattaaaaaa tttaaggggg
    39061 aataaaaatg aagttaaaaa agaataaaaa aggtttcact ttagtggaat tattggtagt
    39121 aattgcaatt ataggtatat tagcagtagt ggcagttcca gctttattta gtaatataaa
    39181 caaggctaag gtagcaagtg ttgagtctga ttatagttca gttaagagtg cagcattatc
    39241 ttattattca gatactaata agataccagt tacaccagat ggtcaaactg gtttaagtgt
    39301 tttagagact tatatggagt ctctgcctga taaagctgat ataggtggaa aatataaatt
    39361 gattaaagtt ggtaataaat tggtattaca gataggtaca aatactgaag gagttacatt
    39421 aacagaggca caatcagcaa agttattgag tgatataggt gaaaataaaa tatatacaag
    39481 cgctacaaca aatagtttgg gaaatccatt aacaagtaat acaaaaatag ataataacgt
    39541 tctatatata gtacttatag ataatactgt gatggacaca acaaaatagt taaaacaata
    39601 tttttataaa aatattgtaa atttaatgaa aaatataata taaaagacag gaagaaaatc
    39661 ttcctgtttt tttatactat tttctttaat gttcaattat gaatgatgat ataattgaga
    39721 tagtaaagga gtgaaacctg tggctaaaaa agttagaata ggagacaaac tggtcgaaaa
    39781 aggatatata acagaagaac agcttaaatg ggcactatca gaacaaaaaa acagtggaaa
    39841 aagactagga gaatttctag tacaagaagg gcttatagat agtaacttac taataagtgt
    39901 attaaaagaa ctcttggata tagaaagtat atttttagaa gggacagaaa tagacacttt
    39961 agctacaaaa atggttccag aaaatatatg caaaagatat actgtatttc catttaaaat
    40021 tgatggaaat aaaatatgtc tagccatgtc agacccacaa gatagagaag cagttcaaga
    40081 tgtaaggcgt atgtctggta aagacgtaga gatatttata tcaagtacag aagatataaa
    40141 caaagcaata ggtcatgctt atgcacattc agaaataaat aaggcaatga ctgagtacaa
    40201 caaaaataga actggtggag taagagaaac agtaatactt gaagaagatg taaatgctgc
    40261 tccaatagta cgattagtaa ataacatact agaaaatgct gttagaatgg aagcaagtga
    40321 tatacatata gaacaaagtg aaaattacat gcgtgttagg tttaggatag atggaatgtt
    40381 aagagaatat atgaggatga actcagcacc atataaggca gtaatcagcc gtatcaagat
    40441 aatgtcagat ataaatatat ctgaaaagag aataccacag gatggtagaa tctatttaaa
    40501 agtagataat aaacccatag attttagggt ttctacaatg ccaactaaca gggatgaaaa
    40561 gatagctatg agagttcttg ataagagtaa ttttatggtt agtaaagaag tactaggaat
    40621 agacgaacat ggctctaaga tatatgatga attgataaac actccttatg gtcttatatt
    40681 ggttgttggc cctactggaa gtggtaaaac cactacattg tattcaatgt taaatcaact
    40741 aaatacagaa aatagaaatc tattaacaat agaagaccct atagagtatg aattacctgg
    40801 tgtaaatcaa tctcaaataa atgaaaaggc agggcttact ttcgccagcg gacttagagc
    40861 ttttatgcgt caagacccag atataataat ggttggagag ataagagata ctgagacagc
    40921 agaaatagca attagagctt ctcttacagg acatttagtt ttaagtacat tacatgcaaa
    40981 tacagcagtt ggagcaattt cacgtctttt agatatggat gtggaaagtt ttttaataac
    41041 ttcatctgtt ttaggtgtaa tatctcaaag acttacacga aaaatatgtg agcattgtaa
    41101 ggtttcatat gaagcagata ttggagaaaa aaaggctctt ggtattgatg taaatgaatc
    41161 agtaactatt tatagaggaa aaggttgtga aaggtgtaat aatactggat ataaaggacg
    41221 tctaggtata tttgaaatgc ttgaaattac tccagaaata aaagagttaa tagattcatc
    41281 tgcaaatcaa agagaaattt taaagatggc tagaaaacaa ggtatggtta gcttaaaaga
    41341 ggatatagtt aaaaaagttt taaatggaaa gacaacagta gaagaaatga ttagaataat
    41401 tttgatgact gattaaagga tgatattatg aaaagtttta aatacacagt aataaaagag
    41461 gacggaaaaa agcaaactga tgtgattgaa gccaacgatt ttaatgaagc tagaaatctt
    41521 cttagaaaga gaaatcttag agttattgaa atcaaagaaa gcaaaaataa aaatataaga
    41581 ttatctagta gaaagagaaa aaaagactta ggagctgacc agataagcca cttttgtaga
    41641 cagtttgcta ttatagtatc ttctggtata aatagtattt ctggattaga aacattagct
    41701 agaaggtcaa ctaacattac attaagagaa gaaattaatc gtatagtggc agaaataaaa
    41761 gtaggttcta ctattgcaga ttcaatgctt tcatcaaaat caaagtttcc aaagttactt
    41821 ggtgctatgg tagctacagg tgaagctaca ggtaaattag atgaagtatt aaaaagtatg
    41881 gcttcatttt atgcaagtga acacagggtt aaacaaaagc ttagaaatgc tgcaacatat
    41941 ccattaatag ttttgatttc ttcatttata atgattttta tatttacaac atttatggtg
    42001 cctaagatga ttaattcaat tacaacagta ggagcaactc ttccacttat aacaagaatt
    42061 gttatggggt ttggtatgtt tatgaagaag ttttggataa tagtactttt agttattata
    42121 ttatttatct atcagtttaa aaaatatcta aaaacgtcag ttggaagggc tcataaagat
    42181 agagttataa ataagatacc tgttttagga aaaggaataa attgtatggt agctacacga
    42241 ttttcaagag cattgtatct atttgtatca acaggatacc cattagttca agggcttgat
    42301 tatattatag atagtgtaaa taacaccatg gcagagaaga ttttagcatc agctaaagat
    42361 ggaataacaa gaggagaagg cttagcagaa aatttagaga gatatacgta ttttgactct
    42421 gtgttggtac agatgatagc tataggggag cagacagggg aattagagaa tataagtaga
    42481 cagatggcag aattttatga gtatgaatcc gagatttatt taaataggat ggcctcaatg
    42541 atagagcctg ttttaataat tgcagttggt attatagttg cgatactggt tgtatcaata
    42601 ttcatgccaa tgcttagtat atatgatgcc atgtaggagg gaaaggacaa atgaacaagg
    42661 tttatgtatc atattatggc gattctattt ccatagttga gggcagatat aaacacgata
    42721 aggataagtt ttatgtaaaa aattttaatg tgttttcaat agaggatatt gcacaagatt
    42781 tttctaagga aaagtatgct ctattgagat acgcattaga aaatgttaag ttaaaatcaa
    42841 gacatgtagt tttttgttta aatacaagag atgtaatttt aaaaccccaa aaacttccta
    42901 atatggacaa aaaggattta gacggattta tgaagctaga gatggacgaa atgatggctt
    42961 tggagtcaga tgaatatgta ttttcgtatg aagtatctag tgaaacacct gataattcag
    43021 atggcgatga atcaagttta aatcttatta ttggagctat acaaaaggaa gaggtaaatg
    43081 caattgtaga gataatgcat gaatttgatt tagtattaga tagaatagat acattatcaa
    43141 cagcttactt aagaatgctt aaaaatttag actacaatga tataatggta accaatataa
    43201 gtgacaatag cacaattata aatatatata agaaagatac tttatttatt agtgataata
    43261 tacctataaa gataactgct aaagatatgg acatgcaatt attaaatgta gtagaagaat
    43321 caaaaggtct tatgaattac tattcgtcaa gaaactttgg taaggttacg gatactatat
    43381 taatattagg aaaaagatat tccaataaat taatttatga aacttttagc tctgcatttt
    43441 caagctatgt ttctcaaggc cttactgaga taatggatat atctgatatg ataactggag
    43501 atatagatga agatgatata aattcaattg tagagctttt aggaagtatg atagaaccta
    43561 aagggaaaag agattttgaa aatataaact ttttaccaat ggatatactt agaaaacaga
    43621 taaaagctaa acaaatgaaa aacatactga aaattgctcc aatagttatt ttgattcttg
    43681 ctatacctta tttatgttta attttttcaa ataatttagc aaataaggaa cttgaaaatg
    43741 ttaatggtga aataagaaat attgaatctg catattatca gattggaaat atagaaaaaa
    43801 tgatacagag taaaagcgaa gaaataaagg tatatgatat gttaattggg aaaaaggtaa
    43861 agtgggaaaa tttattggat gatatagata aaaatactcc aagtaaagtt gaactaacaa
    43921 gtttatcaac aacttatcaa aatcttggaa ctaataataa agctgataat tcaaaaagta
    43981 ataagactac aacagataaa tcaagtaaca atgaaacaaa atcaagtacg aaaagtgata
    44041 gtaatggaga taaaacagat aatgatttaa atgaagataa gactacaaat gtgtcagaag
    44101 cattaactgg aaatagtgag actaatagta ataacacaga taaagatgat aaaactccat
    44161 tgtatgataa gataccaaat gttataaata tagaaggaaa tgctaaagat tcctcttatg
    44221 taggtcaatt tttatataaa ttaaagggat tatactattt caaggatgtt aaattacata
    44281 gcataaaaag taatgcagaa gattctaact atacatttag tatgacatta tacctgaaag
    44341 aaggtgtatt aactaatgaa taaagatata tttaaaaaag atataaaaga tatatttaaa
    44401 atggaagtta gtctaaaagg gattcttata aaggttataa ttttattagc tattttatca
    44461 tatggttatt ttggacttta ccctcaatat cagaaatata ttgctacaaa aaattctgta
    44521 aaacaggcta ctgaaaaatt aaatatatat aaagagaaaa ctgcacttat gccaaaatat
    44581 gaaaaacaac ttgaagaagt tgaaagtgaa ttaaaagaaa aaagcaaaaa tcttttatat
    44641 gatatgcaag atggtctttt tttaataggt ttagataaaa aaatgaaggc ttataatatt
    44701 gacttaacca gttatgacgt tggaaatgtt aaagaatacg aacattttta tggtatatca
    44761 acaacaataa gtgttagagg tgattataga aatgtcaaaa aattaatggc ttatttagaa
    44821 gaacaaaaaa atgttactca aatactagat tatacaatga cttcatatat agaaccagaa
    44881 aaagaagagg aagaaacaac aaacaataag gatttaatta aaaatgtgaa cataaataaa
    44941 gatacaaaaa tatactgggt ggaaggcgaa aataagtggc atttatataa ggattgtcca
    45001 ttgataaaag agggagagat tctaaaaaat gacaaacttt tagataggtc tactaattat
    45061 ctatgtaata cttgtgtact tagagctgca acagaaaaag aagcaaaaaa agaagtcgta
    45121 gctccaaagg cagaaggtgt agtagatgct acatttaaat ttataatgta cactaaagag
    45181 aatactacaa tgatacttga tacagataat ccatcttcat ggagaccagg aaaatataat
    45241 ccatttaagt ctagtgcgaa ataaagctaa gtaagcttta aagataggtg atgagtttgt
    45301 acaaaaataa tgaaaaaggt cttactttat tagaagtaat aatagctgtt tttatactga
    45361 caatagtttt aagtatttct tataaagtgt tcaatggtat aacatcagca gtaaaaaagc
    45421 aacagattat tacagatgct caagtaaata ttaatctaat taataagtat ctaaatagag
    45481 atttggaaaa ctgtaaagaa ctaactaaaa ctggttcagg taataattat gaatacaata
    45541 tagagatgcc agataatgtt gtaaaatatg aagtttctat agaaactaaa aaaaatacag
    45601 aggtatattc tgtaacgaga atccaaaaca atacaattga tacagaaaat gaagttagag
    45661 aagagataat ctataatcaa ccactagttc aaaacaataa agaaatgaag gaaacaccat
    45721 ttaaaataga aaagcaaact ggtaaatcta tttatactgt aagtatatac tataatgaat
    45781 cagtgcaaga aagtcataaa aatagcaatt taaataacaa aacttataca tttgatgtta
    45841 tgtcaagaat aggataataa gaatttatta aaaaataagt aggttgataa aatatgagta
    45901 aaaagaatag tgaaagagga ttttccttaa ttgaagtgtt agtagctatg gctattatgg
    45961 gaattgtact atttgcattt tttaatatta tcaatactaa taacaaagca aacactaaaa
    46021 atgacacaga tataacttcc ttaaactatg tccaaagtga aatagaaaat ctaagagaaa
    46081 agataaaaag tggagaattt gattttgata gtttagataa actagaagat ggaactgtcg
    46141 tatatgaaaa attaatagat aaatcaaaaa aagttgtata tgataaagtt cttagtgaag
    46201 gtgatgtgag cttatatgat actccatatg aaaagattac aacaataaaa gatgaagatg
    46261 gtaatttaat cgacaaagga aatattacaa ataagataaa gactattgta gaagataaaa
    46321 gtggtcagat atataaaata gctgtaactg ggaaaagcat gaatgactat tcaagtaaaa
    46381 aagaggttaa aattgtaact gaaatattta aagataaata attttacttg ggggtgagat
    46441 tttgaggaag tggaataaat ttaaaagtga aagaggggca gctttagttt tagttttggt
    46501 tgtagtagca ttgctttcta ttgttggatt gatattttca aatcagatag ctaatagaat
    46561 aaaatctaca aagactacta atgaaggtat acaagcaaag tatttagcag agacatgtgt
    46621 ggaaaatagt attgataaag catatgaaaa attgtatgat gagcttgaga aaatggataa
    46681 tgagtttaaa agtgaaaatc aagaaaaatc catatctagg tcaaaattaa gagatatatc
    46741 agatgaagat tttaataatc aagatgagga aaatattgaa gcagaaagac ttggatatat
    46801 gaataatata aatttctatt taaataaagc ttcttcagac ttggaaaaag ctagtatgga
    46861 attgaaaaag ctatatgatt tagatatgtt ggactataga gacattgaat atgtagatgc
    46921 aaatattatt tcacatagag attctatctt agaaatttgc aagaattata cttctggaga
    46981 tattagtaaa ataaatgaat atattctgaa agaagatata gactcaacta cattgattga
    47041 ggcaaagtta gtgaataatg atattctttt aaaaatgttt ttagaagaaa ataaaattga
    47101 aaatgaacat ttaaattcag ccttttcaca tacttataag gcattagata acatttcttt
    47161 ggcgatgcaa aatatgattg agtatagaca tacgtttcat atagatgagc caaaagtaga
    47221 agtatctaat gggattccag attctcaaca atattatgaa ctaatacaaa atcctataat
    47281 aaatagtatg gagtatatct ggaatagtaa atgggataca ttagaaaaac tattggaaat
    47341 tttaccaaat caaactcaag gatttaattc tttaagagtt catttgagaa ataatgtaag
    47401 aaaatttgag aagttaagcg ataatatatc tagtggtaaa aaaaatacag caaaaaattt
    47461 cttaaagtat aaagagttat tgtatgaaat atcagaccaa tgtaatcaac taaaaagtat
    47521 gtcttatgag aaaatacctg ttaaatatga taacatggct ttgataacaa cttttgacta
    47581 tatacaaaat gagcttttag cagagataaa atgcagatta aaagaattaa aaccacaaga
    47641 aattgataaa acagaaggta ttactataaa aatacctttt tataaggctg actacgacat
    47701 gacaaaagaa ggatggccta aattaaaaga aaatggttca ggggcggagt taagcttaat
    47761 ggtaactggt gataaagatg gaataaaaga agtagaagtt acagatggta aaaaaaatat
    47821 tattggttta ggtgttgaag aaaattcaaa ttcaaagtat aaggttgacg ccattgtaaa
    47881 ctttaatcta aatatagata caaatgtagt tggaaattat gatataaaag ataaaatttt
    47941 gattaatcat gatataagtt catacaaaaa agtaaactaa atataagaag caacttagta
    48001 taaataaata ataaaataat tttattattt actactaaaa aaataagtaa actaatagaa
    48061 gatatggaga gtaaatatta tgtatattta tgatttatta gagcaaggta taagactaaa
    48121 cgcatcagat atacacataa cagttggaac aaatcctgta gctagagtaa aaggtggatt
    48181 tgtaaaacta agtgaacaaa tattgacatc agaagtaaca atgcaaatgg caaaagacat
    48241 agcaggggaa tctatgttta aagtaataga agaacatgga gaagctgatt tttcagcttc
    48301 tttaaaaaca ggagaaagat ttagggtaaa tgcatatagg caaaaaggaa attacgcaat
    48361 agcaataagg actataacag cagaaattcc aacatttgaa aaattaggcc ttccagagag
    48421 tataaaatct tttactgaaa agcataaagg attagtttta gttacaggtc caacaggaag
    48481 tggtaaaagt acaacacttg caagtatgat aaatataata aatgaaaaac aacaaaaaca
    48541 tataattaca cttgaagacc cgatagaata tgtacatcat cataaacaaa gtttagtaaa
    48601 tcaaagagaa gtaggaactg atacagaaag ttttcattca gcccttagag ctattttgcg
    48661 tcagaaccca gatgtaatac taataggaga gatgagagac cctgaaactg tttcaatagc
    48721 attaacagca gcagaaacag gtcatttagt attttcaaca ctacatacag tgggggcagc
    48781 taagacaatt gatagaatag tagacatgtt tcaaccatcc caacagcaac aaataaaaac
    48841 acagttatct acagtttgtg aaggtgtggt atcacagcag ttacttccta cagcagatgg
    48901 gaaaggcaga atagcagcaa ttgaattgat gtttgctact ccagcaataa aaaacctgat
    48961 aagggaaggc aaaacatatc aaattccaaa catgatacaa actggagtta aagtaggtat
    49021 gaaaactatg gaccaagatt taatggaact ttataaaaat ggaaagatta caaaagatat
    49081 ggcacttagt aggtgtactg accaagagtt tatgacaaga atgataggtg gagtaaacta
    49141 caatggatat tataatcgtt aatctatatg tatttatggt gggtatagtg tttggaagtt
    49201 tctttaacgt ctgtattttt aggattccag aaggtatatc tatttctaga cctccttctc
    49261 attgtatgag ttgtaatact agactaaagc caatagactt aataccagtg ttaagctatt
    49321 taatgagtgg cagaaagtgt agatattgtt atgagaaaat ttctagtaga tatgctatag
    49381 tggaattaat tacaggactt ttatttttaa cagtatttat gttatatgga atatctacat
    49441 caactattta ttatttggtt cttgtgtctt tactgattat aattacattt atagatattg
    49501 accactttat aataccagat aaaatattgg tttttggagc tatattttct atgattttta
    49561 accttttatt taaagaaatt ccagttaagg attcaattct aggtgttttt atttgtggag
    49621 gaagtgtctg gattatagtt cttttgattg aatttgtaat aaaaaaagag tgtatgggtg
    49681 gaggagatat caaactattt gcaatgctag gtttttacat gggtgttaaa aatggtcttt
    49741 tgacagcctt actaagcgtt tatgtgggag cagtctatgg aatctgtgta ataattataa
    49801 gtagaataaa aggaaaggaa tataattctg ttataccata tggacctttt atatctattg
    49861 gagcattaat aacaatctta tgtggaaagc aattacttga actttatttt tctatggtta
    49921 tatagatgat agtcaagtta aataaagttt taatttaaaa aaatgaatta aagtatttaa
    49981 taaggacaat attattatta accctattac taaagtgcta caaactatgg agtatataat
    50041 aataatttcc ttattttttg cacatttgat aaataaatct ctatgtgttt tttgtgatag
    50101 agaaaagaaa aatttataca atacgttttt tatttacata ataactgtag ttttatctgt
    50161 attaatatat ctaaaatttg ggattagtgt agaatgtgta caatatatgg ttttaattcc
    50221 atttttaata attatatcca ttgtagatta ctacacaata tatgtatatg atataacaat
    50281 agttagtggt ataatagttc aaggtataat ttttttggtc tttttcaata tagttcaatt
    50341 tgattatata aatcatatat ttggactatt gataggcttt attatacctt atattattgc
    50401 taaactaaca aaaggattag gctatggaga tgtaggtctc tatagcttat gttgctttgc
    50461 attgggaaat aattatgggt tttatgtgat agttttatca tttattttag cgtttttgtt
    50521 ttttattatt ttatacataa ctaaaatatt taaagatgta gatataagaa agcaaattcc
    50581 ttttacacct tttatttcct tgtcaacaat tttaataata tttacagagt ataatatatt
    50641 taatttttat tataatttgt tatacaaatt tatataacag gaggtaagaa atgtacgtag
    50701 tagtagggtt aggtaatcca ggaaagaagt atgagaagac tagacataac gttggatttg
    50761 atgttataga tattcttgct aaagaataca atattagtgt tactaaaata aagcataaag
    50821 ctttgattgg tgaagggaga gtaggtactg aaaaagtatt attagttaaa ccacaaactt
    50881 atatgaattt aagtggagaa acattaatag atatatataa atattataag gttgatttaa
    50941 gtaatatagt agtagtatat gatgatatag atttagaagt tggaaagata cgtataagaa
    51001 aaaaaggaag tggaggaact cataatggca tgaaatctat aactaagtgc ctaggttcaa
    51061 atgactttcc aagagtgaga gtaggtgtat ctaaaccaga agctggtcaa gacttagcag
    51121 actttgtact ttctagattt agaaaagaag aatctgataa cataaatgaa gcattagaaa
    51181 aagcggcaga tgctattgac tcaataataa gagaaaatat agatatgtct atgaacaaat
    51241 ataatggata gtgagaggga gatttaaatg aatgatgttt ttttatatcc tttgcaaaat
    51301 tcaaaagagt ataaagatat aataaactgt ataaaaaata caaaaggctc attattggtt
    51361 aatggacttt taccagtaca aaaacctcat atatcgtatt ctatatttaa tgatttatca
    51421 cgacaaatga tttttataac aagtagtgat ttagaagcaa agaaagtata cgaagattta
    51481 agtttttata tggaagataa agttgaatat ttaggttttc aagatattta tttttatcac
    51541 ttggatgcaa aggacagaaa cgaagaagcg aagaaattaa aagtattgtt aaaactagca
    51601 aataaagaaa aaatcatttt agtaacttct attgaggcag ttttaagaaa atacatacca
    51661 aaacaagttt tgctcgatag tgtatctcat tataaggtgg gagatagctt agacttagaa
    51721 aagttaactg agaaattagt ttcattggga tatgaaagag tttcaaaaat agaggggttt
    51781 ggtcaattta gtataagagg tggaattatt gatgtatttt cacttgaata tacaaatcca
    51841 attcgtatgg aattatttga tgatgagata gattccatac gaacttttga cgtatattca
    51901 caaaaatcaa ttgataaatt gcaacaattc tctataactc catctaggga atttatttat
    51961 cccgaaaaaa ctacagatgc attagttaaa ttaaaaaaag aaacaactaa aaatactgat
    52021 gaaggtacat ttcaaaatat agattatata tcaagtaaga cttattttga aggtgtagag
    52081 aactatatag attatattta tccagaagaa aacaaaagta tatttactta tttagcagat
    52141 gatgcaatag tatttattaa tgatataacg agacttaaag aaagatgcga aaattatata
    52201 aatgagttta gagaaaatta taaattaaat cttgaaagag gtctggctat taaaaaccaa
    52261 ggtaaattgt tatatcacta tacagattta gagtacttag taaaagacaa gtctgtggta
    52321 ttaaactcgc tattgccaaa gtctataaat aacttttcta taaaatctat aataaatttt
    52381 gaaagtagag aagttcctac atttaatgga aaggtagatt tgcttgtaga agaattaaat
    52441 cggctgaaat ataatggata caaaataatt ttagcaacaa ataccttaga aagagcaaat
    52501 aaattaggaa aagatttatt ggacaaaggc ttagaaacga ccatttctaa agatagagat
    52561 attgaaatta aatcttcaca agttataatt gtacctgcac acataaacag tggttttcag
    52621 tataaatcta taaagtttgt tgttataaca gataacgaaa tgataggtgt ttataaaaga
    52681 gcgtctaaga ctagcaataa aaaagttaaa aagggaaaga aaatagaatc ctttttagat
    52741 ttaagtgttg gagattatgt agtccatgaa aatagtggtg taggtagata cacaggaatt
    52801 gaacaaatta cagtcaatgc tattaaaaaa gattatatga aaatagtata tcaaggtgga
    52861 gataatctat atgtgccaat agaccagatg gacaaagtac aaaaatatat tggtgcagaa
    52921 gtagaaaaag ttaagttaaa taagcttgga acaaatgagt ggacgaaagc aaaagcaaaa
    52981 gttaaaaaag aaattgaaga tatgacaaaa gacttgattg agctttatgc aaaacgtgaa
    53041 aaaatacaag ggtataagtt ttcaaaagat acaccttggc aggcagagtt tgaatcgttg
    53101 tttccatatc aagaaacaga agaccaatta aaggctatag aagaaactaa aaaagatatg
    53161 gaatcaagta aggtaatgga taggctagta tgtggagatg ttggttatgg aaaaacagag
    53221 gtagctatta gaagtatttt taaagcttgt atggaccaaa aacaagttgc tgtattggtt
    53281 ccaacaacaa tattggccca acaacattat aatacattca aagagaggtt tgaaaattat
    53341 cctttgagag tagaagtttt aagtaggttt aaaacaccaa aacaacaaaa acagattatt
    53401 gaagatgcta aaaaaggatt agtagatatt ttaattggaa cacatagaat aatatccaaa
    53461 gatataaatt taccaaactt aggtcttgta gtcattgatg aagaacaaag atttggagta
    53521 aaacataaag aagctttaaa gaaaattaaa tctacagtag atgtacttac actttcagca
    53581 actcctatac caagaacatt acatatgtct ttaagtggaa taagagacat gagtgttata
    53641 gaagaacctc cacaagaaag acatcctgta ataacatatg ttacagagag taaggaaagc
    53701 gtaattcaag atgagataga gagagaatta agtagaggtg gacaggtatt ttttgtatac
    53761 aatagagttg aacacattga agaaatggca agtatgattc aaaaactcgt accagatgct
    53821 agagtggctg ttgcacatgg aagaatgact tctaaatcat tagaaaatat aatcctagga
    53881 tttttaaata aagattatga tgtactagtc tgtactacaa taattgaaac tggtatggac
    53941 atatcaaatg caaatacaat gattatatat gatgcagata aaatgggatt ggctcaactt
    54001 taccaattga gaggtagggt tggaagaagt tcaagacaag gatatgcata tcttttgtat
    54061 gaaaaagata aaactttaag tgagatagcg gagaagagat taaaagctat cagagaattt
    54121 acagagtttg gttctggttt taaaatagca atgagagacc ttgaaataag aggagcagga
    54181 aatattttag gttctcaaca acatggtcat atggcagtta taggttatga tttgtacgtt
    54241 aaaatgctta atgatgcaat taaaaaagtc aaaggtgagc ctattgtgga ggaaattgat
    54301 gttgaaatag atttaagtgt aaatgcatac attccagata attatatcaa agatgagttg
    54361 attaaaattg aaatgtataa gaaaatagct tctattgaaa acaaagaaga catgttggat
    54421 atacaagaag aacttgagga tagattctct gatataccta aaccagtcca gacacttctt
    54481 acaatagctt atataaaatc gttatgtaag atattaaaaa tagaaaagat aagacaatta
    54541 aaagatgaaa tattattagt tccaataaca aagtatagaa ctaaacagaa aataggttat
    54601 aatatagtta cagaattaga agaattatta gaaaagatgt gcaaggtaaa ataacttgta
    54661 aatttaagaa gggatgtttt aaatttgaaa aaagtaataa cattggtaat agctatgatt
    54721 ttggtagtat ctgtgactgc ttgcagttct agtaaaggag aaactgtggc aacagttgaa
    54781 ggtacaaaga tatcatcaga tgaatttaaa aaaactatag ctctttataa agattctatg
    54841 gaacaaactt atggaaaaga tatatgggat aaggaagttg aaaagggtgt aaaatacaag
    54901 gacaagttta aagatttaat tcttgaccaa cttataacta cagaagtaat atattctcaa
    54961 gctaaaaaag ataacttact tcctaaaaaa gaggatgttg aaaaaagttt taaagaatta
    55021 aaagacgcta tgggtaaaga tgaaaaatat aaagaacaac ttaagaaatt aggtatagat
    55081 gatgagtttt taagagacca acaagagaaa gatttagcaa tgcaaaacta tcaaagtaac
    55141 tttgctaaaa agactaaaat atcagatgaa gaaatgaaaa aatactatga tacacataaa
    55201 gatgaattta aaaaagatga ggtagaagct tctcatattt tattgaaaac agtagatgat
    55261 aataacaagc cactttcaga taaggaaaaa gctgaagcta agaaaaaggc tgaagaggct
    55321 cttaaagaag ttaaatcagg tgaggatttt gcaaaagtag caaaaaaata ttcacaagat
    55381 acttcagcaa gtgatggtgg taaattagga ttcttttcaa ggggtcaaat ggttgctgaa
    55441 tttgaagatg ctgctttttc tatgaaaaag ggtgaagtat ctgatttagt tgaaactcaa
    55501 tatggatacc acataattaa agtgacagac agaataaatg agcaaacatc atttgaagat
    55561 gctaaagaaa ctataaaaga ccaactgttg aaaaataaat atcaagaaca aatagaaaaa
    55621 ttaactaaag aagctaaggt agaaaaagat gaaaaagtaa taaataaaat cacaatctaa
    55681 gacttaaaaa aagagtaata tgcttaaaaa agctatatct ggataagata tagctttttt
    55741 tgtatatttt tcttaacaat ggtaaaaata aacttaaagt aatttatgga gggattaaaa
    55801 aatatgaaag caacaggtat agttagaaga atagatgatc ttggaagggt agttatccca
    55861 aaagagataa gaaagacatt aagaattaga gaaggagacc ctttagagat attcacagca
    55921 aaagatggag aagtcatact taaaaaatac tctccaatag gtgaattaaa tgaattttcg
    55981 caagaatatg ctgaaacatt agctgaagta ctaggatatg gagtgttgat aacagactta
    56041 gattctataa tagctgtgtc taagcttcct aaaaaagatt ataaagaaaa aagtataagt
    56101 gatgagttag aagcaataat aagtgaaaga acagtaaaat attcaaaaga taaaaacatt
    56161 ttaccattat tcaaggaaga ccaaaaagaa tatactagtc aagttatcat gcctataatt
    56221 agtgcatctg gtgactgtat aggttcaatt gtagttgttt caaaggataa agatacaatt
    56281 gcagaaagtg aagaaaaatc tttaaaaatt gctgctaact ttttaggaaa acaagttcaa
    56341 taattttatc ataaagacct tttacatgaa ggtcttttga agtttttgtg taaaatattg
    56401 aatatagcat taaaaatatg gtatattaaa gtttgttata taaacagtat ggaggttatg
    56461 ttatgaacaa taacaaaaat aaagactcct ttctaaaagg agcccttata cttgggctag
    56521 caggtgtaat cgtaaaaata atgggtggat tttttaggat tccccttgga aacatgattg
    56581 gttcagaagg aatgggatac tatcaggctg cttatcctgt gtatactcta tttttaacat
    56641 tagctactgc tggatttcca acagctgtgg caaaattggt ttcggaaaaa gttgcaattg
    56701 gaaattttaa aggtgcaaat aagatattta aggtatcaca tacagtttta ttcattaccg
    56761 gtataatatc attttgtata ttattttttg gagccgacta catagtaaca aacgtaatga
    56821 aaaatccagg tgcgttatac tctatgaaag ccattgctcc agcattacta tttgttcctg
    56881 caatgtctgc atatagagga tattttcaag ggagacaaga tatgactaag attgctgtgt
    56941 ctcaagttgc agagcaattt tttagagttg tattaggtct tactttggct tacttgctta
    57001 tgaagagtct aggtcagact tttggtgcag caggtgctat atcaggggct acaataggtt
    57061 caatagcatc tatgctttat ttggtgtttg cttatatgct agggagaaaa gagcgtagag
    57121 cagagataga tgcaagccaa agatttaaag atgaaagagt gtcttatatt tttaaaaagc
    57181 ttttaactgt tgccattcct ataacaatag gagcatcagt aatgccactt gtaaatatga
    57241 tagataatgt tattgtaata agaagactta tggaagcagg ctttacatac aaagtggcaa
    57301 attccatgtt tggtcaatta acaggtatgg caatggccac aataaattta ccagcagtca
    57361 taacaactgc aatgagtatg agcttagttc cagcaatttc taaagcatat gcattaggaa
    57421 ataagtcaaa agctagaaaa gatactaaga gtgcagttaa ggtaacttta cttatagtat
    57481 tgccttgtgc gtttggtatg gcttctttag caataccaat aatgggtctt ttatttccac
    57541 atgaaccatc ttctgtagga actattttgt ttactcttac tccatgtgta ttatttttag
    57601 gattaataca gacattgact ggtataattc agggaatggg aaaaccaata gtacctgtaa
    57661 ttgctttatg tgttggtatg ctatgtaaaa ttgtaataag ttatacttta acaggtatac
    57721 cagatattaa tgtactgggt tcagcatttg gtacagttac agcatacttt gtggcagcca
    57781 tgattaactt actatatgtt aaaaaacaca tgaatgtaaa cttttctaaa aaagaattta
    57841 taataaagcc atttataaca gttatgacaa tgtttataat ggttaagttg agttatggtg
    57901 ctttagttgg attcttagga aacagcatat ctacaataat tgcaatatgt gttggtggaa
    57961 ttgtatatgt agtagtaata cttggaatag gtggaattaa aaaagaagag attctaacta
    58021 tgccaaaagg tgataaatta tataaattgt taaaaaaagt taaacttata agataatata
    58081 ggaatatata tgcaaataaa ggaaatacta tacaaatagt aaatctcctt atttgtataa
    58141 taatataaaa ataaagtgaa atttgctaat aggagtgatg ttatgggtaa gataagtatt
    58201 gtaggtcttg gaccaggaga ctattctctt ataagccaag gtgtgttaga atctttaata
    58261 aatagcccta aagttttttt tagaacagag aaacatccaa taattgataa acttaaagaa
    58321 actatacaat acacttcttt agactacttt tatgaaaaag aggaaaattt tgataatgtt
    58381 tatttgaaaa tatctgaatt tatcatagaa gaatcagcaa agggtgacct agtgtatgct
    58441 gttcctggtc atccaagagt tgcagagaag actgtaagta taatagagag attagccaat
    58501 gaaaagcaca tagcagtaga aatattgcct tctatgagct ttgtagacgc tatgtttaac
    58561 tatttggcta tagacccatc tgagggattt aaactggtag atgcttttga aatagaaaat
    58621 tcatatatag acttggatac aagtatgata ataactcagg tatatgataa atttatagca
    58681 tctaatgtaa agttaaaact tatggaacat tatgactata atcaagaagt atgtatagta
    58741 aatggagctg gtgtcaaaga tttagaaagt aaaaagttta taaagctttg tgaattagac
    58801 agaaatgaaa acttttttga ctatttaact agcttatata tacccaaaag ttctaaaaag
    58861 atgtataata cagtacatga cttagaaata atagttaata cacttagaag tccatctgga
    58921 tgtgaatggg ataaaaaaca aacacatcaa tcattaaaaa attctgttat agaggaagca
    58981 tatgaacttt gtaatgcaat tgataataat gatatagatg aaatggtaga agagttaggt
    59041 gatgttcttt tacaggttat attccattgt cagataggaa atgaggaggg ctactttgat
    59101 ttaaaagaag tagtaaatgg catatgtaaa aaattaattc atagacatcc tcatatattt
    59161 agtggtgcaa aattagatat gtctaagttt gagaaaactt gggaagaatt aaagaaagaa
    59221 gagaaaggcg aaagtactat aacagatggc cttagaagga taccaaaaca tttaccagct
    59281 cttatgaagg ctagtaaagt tcaacataaa gcagcacttg tcggctttga ttgggacaat
    59341 gtagaagatg tatttaaaaa aattgaagaa gaatatcagg aattacttga cgaacacagg
    59401 gtcgggaata taaaatacat aaaggaagag ctaggcgatt tattattttc gatagtaaat
    59461 ctagctagat ttttagacat agactcagga gaagccttga actgtactac agaaaaattc
    59521 ataaatagat ttgactttat agaaaagagt gcaatgaact tgaataaaaa attagaagac
    59581 atgacacttg aagagatgga tgagttttgg aatcaggcta aaaaacaata aaattgtgga
    59641 aggaaatttg tcaaaaatct agaactttta tcacaaagat agaagtctat attataaaca
    59701 tttttaggag gtaaatatcg tgaataaagc tgaattagta tcaaagatgg cagaaaaaag
    59761 tggattaaca aagaaggaag cagaagctgc gttaaacgca tttatgagtt ctgttcaaga
    59821 tgcactagta aataatgaaa aagttcaatt agttggattt ggaacatttg agacaagaga
    59881 aagagctgct agacaaggaa gaaatccaag agatccagag caagttatag atataccagc
    59941 ttctaaagca ccagttttca aagctggaaa aggattaaag gatataataa atggataatt
    60001 agaaatcttg tgaaaaagtg tggataaatt tgccacactt tttctataga accaaggaag
    60061 gtgataatta tgagattaga taaattttta aaagtatcta ggattataaa aagaagaact
    60121 gttgctaaag atgcatgtga taaagggatt gtcactataa atggaaaagt tgctaaatct
    60181 tcttcagagg tgaatattgg agatacatta gaaatacaat ttggagagaa gaagatgaag
    60241 tttaagataa atgaagtaaa agagcatgta ttaaaaaatg atgcaaaaga aatgtacgaa
    60301 attttagaat aaaattaata gtcctctagt atattaatag tagtattatt gaaatattta
    60361 ctggggggat attatggaac agaacataac tttgaaagat agatcaaagc tggttatttc
    60421 gggagtcgag cacatatact cttttaatga caaaagagta gagctcaaaa cttctgtagg
    60481 cgagatggta attgaaggcg aaaacctaga tatgagcaag ctaagtatag acgaaaatat
    60541 aattagcata gatggaacta taaattctat ggtatatgca aagcctaaaa agccacaaga
    60601 gagtttcttg aaaaaggtat ttagatagat gatacctttt actcaagatg taagcatttt
    60661 ttatgctact atttatggtg gaatactcat aggggtgttg tttgattttt atagagggct
    60721 aagagggaat tttaaattca tcaattattt tgctataata tttgatgtat tgttttggtt
    60781 tctagctacg gttataatat tcgtaacaat aaaccttaca gaattttttg atttaagata
    60841 ttatcatttt gtggctttgt ttattggttt tattttatac tataacacca taagtaaaat
    60901 tgttttatca attatcaata aaataattcg ttttgtgaga aattctttta aaaaagttac
    60961 acattacata gtcagttttt taaataattt gtattacgtt ataatctatt cactacacct
    61021 actatttgat attatatttt atataccaaa catatttata gcaacaagaa aaagtataaa
    61081 aagaaaatca aataaaaaat taaagaacaa gaaaaaatca aaacctaaga agaaaaaaaa
    61141 taaaactaaa aaaagggtgt aggaaaaatg aacttaagaa aaaaattttc aggacagatt
    61201 attgttatca gtttattttt aggaatatca atttttagta tgatgacagg atttgtattt
    61261 gagtatacaa aagctaagga atataaaaaa gaaatagcaa gtctaaataa acaattaaaa
    61321 aagactgaaa tacaaataaa cagcttaaag aaagatgaaa aatcatatga aggagactta
    61381 gaggatatag caagaaaaag attgaatatg gtaaaaccaa atgaaacagt ttatgtagac
    61441 ataaatcgtt gattaaaact tgctaataag tttacagatg tattgatttt aaaatttaag
    61501 atttaataaa aaagtcattc agttaaggat ggctttttgt ttttattgag gagggaattt
    61561 cataatgaaa gtaggagcta tagatattgg gacaaactca atgagattat tgatagcaga
    61621 ttatagagat gggaatttta taaacagaaa aaaatttgtt aatacaacta gaattggtta
    61681 tggtgtggat agtaaaggat atataaaaga agatgctatc aaaagaaata taaaagcatt
    61741 aaaagaattt tctgataagt gtaatgatga aaagtgtgaa aaaatttatt gtatggggac
    61801 atctgctctt agagacagta aaaatggaga ttactttgtt aaattagcta aagaagaaac
    61861 tggaatagat gttgatatag ttacgggaat agaagaatca aatttgggct ttaagggtgt
    61921 tttagaagga atacaaggtg atgatactat tattgttata gatattggag gaggttcaac
    61981 agaatttatt gtaggtgatt cagaaggaat tatatttaat aaaagtgaga atgtaggtgc
    62041 tcttaggatg acagagaggt ttttgaagca cgatattatt gaagatgaag agtttgaagc
    62101 tatgagttca tttatatatg gtgagattga taaaacgtta aatcatataa aagacaaagg
    62161 attaaaaact cttataggta taggaggtac gattacatca ttatcagcaa tgaatcaaga
    62221 attagagatt tactctatgg aaaaaataca taatagtaaa atacacaaaa aagaaataga
    62281 agaaattcta caaaaattaa taaagatgac attaagtgat aaaaagaact taaaagggct
    62341 acaagaaaaa cgtgcggata ttataactgc tggtgtaaaa atattgaata taataatgga
    62401 aaaattagaa atagaagaaa taaccgtaag tgaatacgat aatttggaag gcttaatatg
    62461 tcaaaagtca aaaaaaatgt attaatattt tttgattttt aaaaccgatt atgacaaaaa
    62521 aaattactcc tctttgttac aattcttaat ataaacaaaa gaggaggttt ttttatgcaa
    62581 agaagtgtag cagttgtgaa gaataagact gtaaaaccaa atgtcataaa attaataagc
    62641 ggatatattg attttactac ggcggcattc gctgtgatgg gatttttact tagtaggtct
    62701 attatagtag actctgtagc tcctttagga attgcctttt ttatatgtgc atcaaagatt
    62761 gataagtaca agatacctgt attcatatca acattattag gaatattgtt atcatctaat
    62821 gattttggaa atgtagcaaa atatgctgtg tgccttacaa tatttatggc tatgaataaa
    62881 aaattaaaat tatcagattc aattgctaag gttgcattta taggtgcaat aatactactt
    62941 ccaatctcca tgggacaagc tttcttttca aataggtatg tatatggtct tcttatagca
    63001 ggagtagaag caataataat gtttatatct atatatgtgt tttcttatgg gataggttta
    63061 atggccaatg tcaacaatag gatgtctgta cggacagaag aagccatatc aataagttta
    63121 ctgctaacct ttagcataat gggaatagga gatatatctc tgtttggaat atcaataagg
    63181 accattttat cgaccgtatt gatattgctg acctcaatag taggtggagc tactatggga
    63241 gcatctagtg gtgtaattat tggtgtagca tccattttaa ataatatgac atcagctgtt
    63301 tatatgggga tatattcttt ttcaggatta ataagtggag catttaataa aataaataaa
    63361 tatttctgta tactaggata tattctaagt tggacaataa tatacctata tacttctggt
    63421 attacttcta atatgatgca attaagagat atactattag gttgtttgat agtactagta
    63481 ttgccagagc gattatttaa taagattgaa aaattaataa aaagtaatgt ggcttcaaat
    63541 gaaatcgtat acgattatat tatgagaagc aaaaacctaa ctaatagcag attgaacagt
    63601 atttataaaa cttatgatga ccttgcagat acatttgata agataagaga aaaagacaaa
    63661 gtgctagacc aaagagacat agctaatgta atagatatga ttcataatga tgaatgtaaa
    63721 agttgtagta tgagaagaat gtgctgggag tcaagattta atcatacata cactatgata
    63781 tataacattc ttgaaaaaat tgaggagaaa ggggagttaa gtctaaacga tatacctaaa
    63841 aattttagaa aggaatgtat gaaaccagag tcaatagtga aaatatcaaa tcactattac
    63901 aaaatgtttg tattagatta tgattggagt gtgaagtttt cagaaagtag aaagttaata
    63961 gcaaatcaga taagaagtat ttcaaaatcc attaaatcct tatctcaaga tttagaaggc
    64021 gacataatgc tagatataga aaaggaaaaa aatatatatg aacagttaga aagatatgat
    64081 ataacagttg ataaagtaag ttacttaaca aaaagtaaca gtgagtttga aatatcaata
    64141 gaaaagaaaa cttgccatga tggatgtatg tgtgaagata aaattgtaaa tataatttca
    64201 gacctagtag gggaaaatat gtctgttaga aaaataggat gtcactgttt aggaggtaaa
    64261 tgcaaggcaa cattcgttaa atctcaaaaa tataaggctg ttacagaagt ttcagcaatg
    64321 tcaagggatg ggcatatcct ttgtggagac aattacacat acatggagat aaatgatggt
    64381 aaatacatga tggctataag tgatggtatg ggaaaaggta aaaaagcata tgaagaatct
    64441 tctgccacta ttgatatact ggaaaaaatg atagatgcaa aaattaaaga tgagatagtc
    64501 attgatacta taaataatat gttgttgtta aagtcttcag aagagatgtt ttcaactctg
    64561 gatttgggaa ttttagattt aaaacgaggt tgtttagaaa ctataaagat gggagcgtgt
    64621 tctacttata taaaaagaga ggatggagaa gttgacttaa tatcgtcatc atcattacct
    64681 gtgggtatat tatcagatgt aaaaattgat agaaaaaatg taaaagttaa ggaaggagac
    64741 tatgttataa tggtttctga tggaattgta gatgcaggaa gaaataacaa tttaggagac
    64801 aattggttaa tatacttctt aaagaatata gagactacaa atccaaagga aatttcaaat
    64861 ttaatattgg atagagcatt agagttacaa gcgttacaaa ttgaagatga catgactgtt
    64921 ttagttacga aaatatgtac aaaataaggt ttacaagatt atgtgtataa gatgtggata
    64981 acttgtgtat aatatgttaa tagacttttg aaaaaagtat agttattaac aaaaataaca
    65041 tataaatcaa caaaatttgt tgataagtat ggcagaaatg ttaataaaat gtaaaaaaag
    65101 aaaaaacaaa agctattatc aaaagagatt catgttgaaa taactaaatt tctaagtaaa
    65161 gaaaacctgt gaatatgttg attattatgt ggataactat taattcgata tttatttata
    65221 tagtatgaat aattgaataa tatatctaaa aaaagccatc ctattattaa gtaaacttat
    65281 tgaaaggatg gctttttatg aatagaaaat tgtgcaaagc aataatacct ttatttattt
    65341 ctattttttg catgttaata ataagtataa aagctgacca aggatatcaa aagaataatg
    65401 atagagcaat tactgtcatg aataataatg atactgaaga tgatgctgaa aaaaaacaag
    65461 aaatagacta tgaaaaaaca tgttggaatt tgcaatcttt gtttaaaagt gatgaccaat
    65521 ggaaaaaaga acttaagagt tttgataaag atactaaaga gttgaaaaat tacataggta
    65581 aagtaacaaa atcgccaaca catttgtctt ttgccttaaa cattaaagaa aaacttgata
    65641 taagactaaa taaactttct gcctatgtaa aattaaaaca agatactaat aaaaattcgt
    65701 ataagtactt agatatgaat gatagtatga gtaaatccta tgggaattat atggggattt
    65761 gttcagattt agagttagaa atactaaaat tatcaaacaa agattacaag aaaattattt
    65821 caaataaaaa tataaatagg aaatatggtg catatttaag agatataaga aggaataaag
    65881 tacattattt ggatgataaa tcagaagata ttctaagcaa agtatcttca ataagtagtc
    65941 ttccaagcca agtatatgaa ttatttagaa atatggatag aaaaactaat ttgacaccag
    66001 ctcaatatgc gagtgaatta gagtcatcag atagagaaaa tagaaaaaaa gcttatcaag
    66061 atgaacttat aacttacaat gataatataa atacaattgc aggtttaatt actggtcaag
    66121 ttaaaaagaa tattttttat tctcaggaaa gaggttatga gtcttcttta gaaatgtacc
    66181 tggaatctga taatgtagat gaaaaagtat acaataattt gataaggact gtaaatagta
    66241 atatggacag tctacacaaa tatatagatt taagaaaaaa agtattaaaa ttagataagg
    66301 ttcattcata tgatatgttt gtgcctatgg ttaaggctgt tgataataat attaattatg
    66361 aaaaggcaca aagtatagta tattcagcgt tgtcaccttt aggaaaagaa tacaatgatg
    66421 tagtatataa agctttcaat gaaaaatgga tagatgttta ttcacatgaa aataaagtaa
    66481 gtggtggata ctgcttatct gtttatgaaa atcatccata tatactttta aattataata
    66541 attcattagg ttcagtttcg actttatcac atgagcttgg acatgctgta tatgaatatt
    66601 taagttctaa gaatcagaat tattttaatt caaatccgtc tatatttact cacgaagttg
    66661 catctacaac gaatgaagca ttactttacg agatgcttat aaaagaagct aaaggcaatg
    66721 atgaaaaagc ttattatata acacagtatt tagacttgat aaaagatact ctttatacac
    66781 aaactatgta tgctgaattt gaaaaaacta tacacgagtt agtagaaaaa ggaaaaagtg
    66841 taaacgcatt agtattaaat gatgtttggg gacaacttct taaaaaatat tatggacaaa
    66901 gctatgaatt agaccaatta tctaaggtcg gatgggctag aatcccacat ttttataata
    66961 gtttctatgt ctataaatat gctacaggtt gtagtgcagg agtaagtttt gcccaagaca
    67021 tcctaaagaa tggttcagat aactatatga gctttcttaa aaagggaagt tcagattatc
    67081 caataaactt gttaaaatca agtggcataa atttaactaa tacaaaaccc atagaaaata
    67141 caatcaaaaa atttgatagt ttagtagcag aacttgaaaa gctattataa acattattgt
    67201 aattcacatg gtaaatatat cgaattaatg ataaaatata ctaaaagttt aaagtcctat
    67261 caagatagga ctttttattt attaattagt gggagatgat tctatgcaag ttaaagttaa
    67321 aaaggctgta ataccagcag caggtttagg aactagattt ttgccagcta caaaggcaca
    67381 accaaaagag atgttaccaa tagttgataa gccaactcta caatacataa tagaagaagc
    67441 tgtggcctca ggaatagagg aaatactaat aataacaggt agaaataaaa aatctattga
    67501 agaccacttt gataaatctg tagaattgga acttgattta gagaaaaaag gtaagaaaga
    67561 attgttggag gtagtacaaa acatatctaa catgattaac atacattata taagacaaaa
    67621 agagccaaaa gggttgggag atgcaattta ttgtgcgagg cattttatag gtgatgagcc
    67681 gtttgcagta atgttagggg atgatattgt agataatgat gtaccttgtc taaaacaact
    67741 aacagatgca tatgaggaat atagaactac aatattgggt gttcaaaagg taaatcaaga
    67801 agatactaat aaatatggga taatagaagc aaaaaatata gaaggtagag tttacaaggt
    67861 aaaagacatg gtagaaaagc cagagtcagg aaaagctcct tcaaatattg ctattttagg
    67921 tagatatata ataactcctg aaatctttga tattcttaag gatttaccac caggtaaagg
    67981 tggagaggta caacttacag atgctttaaa aatattatct aagaaagaag ctatgtatgc
    68041 atacaatttt gaaggcaaga gatatgatgt tggggacaag ctaggatttt tagaagctac
    68101 tgttgacttt gctctaaaga aagaagacct taaggaagat tttataaaat atttgaaaca
    68161 tgtatgtagt gaatttgata aaaataacaa tgtgttatac aataatacag atgaaaaaat
    68221 agatttaatt gaagtcaatg aagctagaga acttgaaatt caaaaataat taatgaaagg
    68281 tgatgtatgg tggcaaaatc aagtcagaaa agaattaata gaataatagc tgttgggatt
    68341 gtgggaatcc tcgtaataac gagtataaca gctgttttga gtatgattgc aactatgtaa
    68401 taagataaat gacagcagtt gatatacata aatataaaaa ccatatataa cttgatgtag
    68461 tcaaaaactt acaatatttt agttatatat ggtttttaga atttataaat gtaaaaaaaa
    68521 atttttataa tagttgcata ttttaaagga aaatattatt ataagatata aatatagtga
    68581 taaatttata aaaacgtgct aagattggac ttgaaataca gtaatatagt aggtaataat
    68641 gttttaaatt ttatttattg accataatat ataaataatg atattattag acttaactgt
    68701 ttaaataaaa cttttctttt tcatgccttc aatttccaaa ataagtatgt atataaatga
    68761 atatatagat gaggaggatt tttttgattt tagatgataa agtattagaa ggattaggta
    68821 gtaagacctt agttgagcta cgagagatag ctaaagaatt aaaaataaaa tcaattacta
    68881 catataagaa aaatgagtta attgagatta taaattccaa aagtaaaaat gaggaaaaag
    68941 taaacgaaga gactaaagaa aaaaatgaag atattaaaga agacataaag aatagaaaag
    69001 aaaatataga gcgtgaaagt atagaaaaag aagttgaata taagagccct accaaatcat
    69061 acaacaagaa agaaatagat acccaaaata tttcccaaaa ccaagaacat agaaatcaaa
    69121 gaaatgacta taataagaca acgagtgtaa atgatagtaa taagtcatca aatatatcta
    69181 atagaatggt cagaaataat aataaaaact attatatgcc taaacaagtt gatgaatcta
    69241 agatagtaga tgaatttaac acatcaaaag aggatgaagt agtaggtgta cttgaaattc
    69301 tgccagatgg atttggtttt ttaagaggtt caaattattt gtctactgaa ggagatgttt
    69361 acgtttcacc atctcaaata agaagattta atatgaaaac aggagacaag attaagggaa
    69421 taactagaca tccaaaaagt ggagagaaat ttagagcact tttatatgtt caaaaaataa
    69481 atgatgaaaa ccctgacaca gctatacaaa gaaatgcgtt tgagacatta acaccaatat
    69541 ttccagaaga acgacttaca ctggaaacta atagaaatga aatagctaca agaattatag
    69601 atttaatttc acctatagga aaaggacaaa gaggattgat agtagcacct ccaaaagctg
    69661 ggaaaacagt tcttttaaaa agcgttgcaa atagcatagc taaaaatcat ccaaatgttg
    69721 aacttatagt actccttata gatgaaagac cagaggaagt tactgatatg aaagaatcta
    69781 ttgaaggaga cgttatatat tccacttttg accaagtatc aagtcatcat gtcaaggttg
    69841 cagagatggt gttaaataga gctcaaagat tagttgaaca tggtaaagat gtagtaattt
    69901 tactagatag tataaccaga ttggcaaggg catataacct tacaatatct cctacaggta
    69961 gaactttatc tggaggtatt gaccctggtg cacttcatgg acctaaaaag ttctttggtg
    70021 cagcaaggaa tattagacag ggtggttctt tgacaatatt aggaacagca ttggttgaaa
    70081 ctggttctag gatggatgat gtaatatttg aggaatttaa aggtacagga aatatggagc
    70141 tacatttaga tagaaaatta gcagaaaaaa gaatattccc tgcaatagat atttacaagt
    70201 ctggtacaag aagagatgat ttattgcttg atgatgaaga aaagactgct ctttggagat
    70261 taagaagaga aatgagtaat aattcagtaa tggaaattac agataaagtc attgaactta
    70321 taaagagaac taaggacaat aaagagtttg taaaatcaat aaagaatttg taaaatcaat
    70381 aatacaaatg caaatatgga ttatataaac taatagaaaa atcacttgaa tggcataata
    70441 agttgtgcta taataatgta gttgataatg taaaaagaaa gttaatataa ttaatttttt
    70501 taaataaaga gaagaggtga ctataatgca aaaggaaata caaccaaaat acaatccagt
    70561 tgaagtgcgt tgtgcttgtg ggaacacatt tgttgctggt tcaactaagg acgaaataaa
    70621 agttgaaata tgttcagagt gccatccatt ctatacagga aaacaaaaga acatagaaaa
    70681 aggtggaaga atcgacaaat tcaagaaaag attcaaaatg gactaatttt tataaaagtg
    70741 gagttggagg aagccaactc ctattttatt acaaaaaaac attcatattc tactttaaat
    70801 tgtctttttg tattataata ataaatatgt aaaattgagt attctaaata caataaaatg
    70861 aggtgagtgg atggcaaaac aagctgtagg tgggcaagct gttattgaag gtgtaatgat
    70921 gcagtcaaaa gacaaaagag cggtagctgt tagaaaaagt gacggagaaa tagcactaaa
    70981 agaagataga ataaaaagtt gggtaagaga taagaatata gataagatac cttttgttag
    71041 aggttctttt gttatgatag atacaatgat tcaaggcata aaaagtttaa acttttcttc
    71101 agagtttttt atggaagaag ctgaagagga taaatttgat ttatttataa agaaaatttt
    71161 taaagataag gcaaatgata taattataat attttcattg gtcatagcaa tgttactttc
    71221 tgctggatta ttcattttta tacctacatt ggtaggagga gctttttcta aagtaatgcc
    71281 taatgatttt atgcttaatt taatagaagg aatcataagg atagctatat tatttgcata
    71341 tatagtattg atttcacgaa gtaaggatat agaaagagtc tttcaatatc atggagcaga
    71401 acataaatct atatattgct atgaaaatga tttagaactt acagtagaga atgctagaaa
    71461 atttaaaaga ttacatccta gatgtggtac aaatttccta tttatagtta tggctgtatc
    71521 aataatattg tttgcatttt ttggttggcc aaatccaatt cttagaatat ttatgagaat
    71581 tatatgtgtg cctatagtag caggtttatc ttatgaggta attagagttc ttgggaaata
    71641 tgataatgga tttactaaaa ttattgcata tccaggaatg atgttgcaat attttacaac
    71701 aaaagagcct gatgatgagc aattagaggt tgctttggaa gcattgaaag cagtagtaga
    71761 ttagagaaaa ggatatttgt tatgacgata aaagatataa ttattaaata ttcagataaa
    71821 ctaaaagata taagtgatac tccaagatta gatacagagt tattgttaca aaaaacattg
    71881 ggtgttgata gattatatat tcacttaaat ctgaataaag agctaactga agaacaaaaa
    71941 acaaaattta tgggttttgc agaagaaaga ttaaatggaa gaccaatagc atatatagtt
    72001 gaaaataggg agtttatggg attagatttt tttgttaagg aaggcgtatt gattccaaga
    72061 ccagatacag aaactttagt agaagaaata attgaaatat gtagagaaaa aaaagatgta
    72121 agtatattag atataggaac tggttctgga gctattacaa taagtttagc aaagtacatt
    72181 gaaaattcta aaatcatgtc ttttgatata tcagagacag cattagaaat agctaaaaaa
    72241 aatgctatta taaatgaagt tggtgaaaaa ataaagtata ttaattctga tttatttaca
    72301 gcaataagtg atagtaacat aaaattcgat atcatagttt caaatccacc atatattaaa
    72361 aaacaagata tagaaactct gcacaaacaa gttaaagatt atgaaccata taatgctctt
    72421 gaaggtggag aagatggatt agacttttat agaagaataa cagaacaagg taagaaatat
    72481 ttaaataagt gtggaatatt agcatatgaa gttggtcata atcaagctga agatgtaata
    72541 aatattatga aaagtaatgg atataaaaaa atatacacaa agaaggatat tcaaggcatt
    72601 gatagagttg ttataggtta caatatatga tataatcaac aattgatatg ttacatttaa
    72661 tcgaggtgaa agtatgttaa aaaaattaga ggtattggaa gatacatata aagacttaag
    72721 tgaaaaaata ggtgatccgg atgtaataaa tgatcaaaaa gtttggcaga aatatataaa
    72781 agaacatgct gacttggaac ctattgttat gaaatacaga gagtataaaa gcgttttaga
    72841 tagtataaaa gaatcaaaag aaattttaca agaagagtca gatgaagaat taagagaatt
    72901 agctaaaatg gagttagctg agatggaaga aaaagtagca cctctggagg aagaaataaa
    72961 aatactatta ttgcctaaag accctaatga tgataagaac gttatagttg aaattagagg
    73021 tggagctgga ggagatgaag cagctctatt tgctggtgat ttatttagaa tgtactcaag
    73081 gtatgctgaa agaagaagat ggaaaataga attattgagt gcaagtgata ctggagttgg
    73141 tggatataaa gaagtatctt ttatgataaa aggtaaaggt gcttattcaa gattaaagta
    73201 tgaatctgga gttcatagag ttcaaagaat accatcaact gaatctggtg gaagaataca
    73261 cacatcaact tctactgttg cagtattacc agaagttgaa gatgtagagg ttgaaataaa
    73321 tccaaatgat ttaagaatag atgttttccg ttcttcaggt aatggaggac aaagtgtcaa
    73381 tactactgac tctgctgtta gggtaacaca tatacctaca ggtgaagtag tatcttgtca
    73441 agatggaaaa tcacaattaa aaaataaaga gcaagcttta aaaatattaa aagctagact
    73501 ttacgataaa gcattggcag aacaacacaa agatatagca gcagaaagaa aaagccaagt
    73561 cggaacagga gacaggtcag aaagaataag aacatataat ttccctcaag gtagaataag
    73621 tgaccataga ataaatttaa ctttatataa gttagatgca ttcttagatg gagatataga
    73681 tgaaatgata gatgctttaa ttactgttga ccaaacagaa aaaatgactg caatataagt
    73741 aatgttttaa agaagctgtt tcttcctaga aattattttc tattttgaaa cagctatttt
    73801 gcattttagg tcatgttatc atattctatt gtatataatt gttatataca agtataggag
    73861 ggttatatga tactaaaagt aacaataatt gggcttttgg caggtgttat agggacagga
    73921 ttaggaggag taatatcagc catttttaaa agagaggtcg ataaatatct aagttttttt
    73981 atgggattat ctggtggaat aatgttggct gtagtggtgt ttgatttaat gaaagaatcc
    74041 atggacaaaa tggggataat caatacagtt atatttacgt ttgttggagc tctcataact
    74101 atgtacataa aaacaaaatt agatgtgtct ggaaatatgg catctggata tcttatattc
    74161 ataagtatat tactacataa tttaccagaa ggactagcca ttggttcttc ttttatgtca
    74221 actgaaagtt taggtattac acttgctata gttataggtc tacataatat accagaaggg
    74281 ttagctatgg cattaggctt agtttgcaat aaaatgaaac taagtaaagt tattttgttt
    74341 actgtaatag ctggattacc tatgggtcta ggaagttttt taggtgttta ttttggagga
    74401 gtatttactt ctcttatagg agtatttctt gctacagcag gaggtactat gatgtatgtt
    74461 gtattggaag aaatttttcc acattcaaaa agtgtgtatt gtataattgg tttcttgtta
    74521 ggtacaatga ttgtaaatta tatataaaat taaaatgaaa gcaggcatac aaaagatgaa
    74581 aactattata agtaatattg atataaataa tattgattat gaggaaataa aaatacaagc
    74641 aaaacttctt agagaaggta aaacggttat ttttccaact gagacagtat atgggttggg
    74701 tgcaaatgca ttagatgaaa atgcagtaaa gaagatttat gaagctaaag gaagacctag
    74761 tgataatcca ttgatagtcc atatatatga aaaagaagaa gtatatgact tagccaaaga
    74821 tataagtgat aaagctaaac ttgttataga aaagctttgg ccaggaccta taacaattat
    74881 attaaataaa aaagatataa ttccatacaa aactagtgga gggcttgata cagttgcaat
    74941 aaggatgcct tctaatgtta ttgccagggc aataataaaa gaagctggta tacctattgc
    75001 agctccttct gcaaatatct caggtcgccc atctccaaca aaagcaaaac atgtatatga
    75061 agagatgaat ggaagagtgg atggaatagt tttaggagga gacagtaatt ttggattgga
    75121 gtcaactgta ttggatttaa cagaagagac tcctatgata ctgagaccag gaagtataac
    75181 taaagaagtt ttagaggaac ttcttggaga agtaaagctt gacccatcat taagtaaaaa
    75241 agaagataat caaaaagcta aagctcctgg aatgaaatat aaacattatt ctccaaatgc
    75301 agatgtatat ataatatctg gaaaaagaga aaatgtagca acaaagatta atgatatgat
    75361 aaaatctaat agagacaaag gtctaaagac aggtgtaatg tgtattagta gaaaccgaga
    75421 gttttatgat ggagaagtaa tcgatttagg aaactctctt gaagaagttg caagtaatct
    75481 gtttgatgct cttatagaaa tggacaaaaa gggtgttgat gtcatatatt cagaagagtt
    75541 ccccaagact ggagttggac aagcaattat gaatagacta ctgaagtcag caggatacaa
    75601 agtgataaag gcttaagagg gagttgtaaa atatgaatat acttattgtg tgtacaggta
    75661 atacttgtag aagccctatg gctgaggcta tactaaggaa agcaattaaa gaatctggaa
    75721 gaagtattga agaatattct atatcatcag caggtatatc tacagcaaat ggaatgggtg
    75781 cttctgaaaa ttctatagaa gtactaaaag aaatcggtat agacttatca aatcatagaa
    75841 gtaaagtaat tactaaaaaa ttaattgatg aatcagatat aatactaaca atgactaaat
    75901 ctcataaaga aattttagta caagcagttc caaaatgcaa agaaaaagtg tatactttta
    75961 aagggtttgc aaataaaaat gaggaagata tatctgaccc atttggtgga aatttggata
    76021 tttatagaag cactatgaga gaaataatgt attctgtcaa cgaaatagta aaaaaaatat
    76081 gaaacggagg ataaacaaat gaagatagga ttaggctgtg accatggagg atataattta
    76141 aaaaaagaaa taataagcta tttagaaagt aaaggtatag agtgtgtaga ttatggaact
    76201 aataatgcta cagattctgt tgattatcct gtttatggag aaatagttgc aaattcagtt
    76261 ataaataaag aagtagatta tggaatatta tgttgtggta cagggatagg aatatcttta
    76321 gctgctaata aggttcctgg gataagatgt gcagttgtat cagatgtatt ttctgcaaaa
    76381 atgtcaaaag cacataatga tgcaaatatg ttatcattag gcgaaagagt tttaggaaaa
    76441 ggtctagcac ttgagatagt tgaagcatgg attaatacgg attttgaagg tgatagacat
    76501 gctagaagag taaacatgat taaatcaata gaagaaaaac ataataaata ataaaaatta
    76561 cagttttatc aggaggaatt atcaaaatga gcaaagtggt agagacaaac catccattaa
    76621 tacaacataa attaacttta atgagagata aaaatactgg ttctaaagac tttagagagc
    76681 ttttaactga aattgcaatg ttaatggggt atgaaataac aaaagatata ccattaaaag
    76741 atgtagaaat agaaactcct attcaaaaaa catcatctaa agtagttgca ggtaaaaaat
    76801 tagcaataat tccaatttta agagcaggtc ttggaatggt agatggatta gtgagcttaa
    76861 tgccagcagc aaaagttgga catgtaggac tatatagaga cccagaaaca ttaaaaccag
    76921 ttgaatatta ttgtaagctt cctcaagaca taggagaaag agatataata gtagttgacc
    76981 ctatgcttgc aacagggggt tctgctgtag cagctataga tttacttaag tctaaaggag
    77041 ctaaaagtat aaaattagct aatttagtag ctgcaccaga aggtatagca gaagtgcaaa
    77101 aatatcatga tgatgttgat atatatgttg catcagttga cgaaagatta aatgaacatg
    77161 gatatataat tcctggtctt ggtgatgctg gagatagatt gtttggaact aaataaaaat
    77221 aaataactat aaatatttat tagggacttt cttatatagt gtatataatt ttatgtaaga
    77281 aggttctttt ttatatattt tttctcaaaa taggaattat tgaattgata aatttctcga
    77341 attgagaata aaaagttttt accaattaaa aaagattgct tattttacaa ggttttatag
    77401 aatagtagtc tattttaatg tggcatgtta gttgcttacg attataggta ttaagtgtat
    77461 tagttaaatt gagaattgaa tattaataat attattggga gtggtaatac atgaagaatt
    77521 gtatagtagt aagaggggct ggagatttag caacagcagt tattcataaa ttacacaaca
    77581 gtggttttaa agtagttgcg ttagaaatag aagagccatt agctataaga aggcaaattt
    77641 ctttttgtga ggctgtttat gaaaaaaaag taactgttga aggtgtagaa tgtatatttt
    77701 gcaatactga aaaagaaata gaggaagttt tatctcaaca taaagtagcc ttgattatag
    77761 acccaaaagg agaaaaatta tctaaattaa atccaatcgt tttggttgat ggaatactcg
    77821 ctaagaaaaa cttaggtaca actaaagata tggcaccact tacaatagct ttaggtcctg
    77881 gattttgtgc aggagaagat gtagatgttg ttatagagac aatgagaggt cataatctag
    77941 gaagaataat aactaatggt agagcagaaa aaaatacagg agtaccagga gcaataaaag
    78001 gtttttctaa agagagagtt atgcattcac agtatgatgg cataatggaa aatgtatgta
    78061 agataggtga tatagttgaa aagggagacc tactttgcta tattaataat tatgaaaaaa
    78121 aacatgaagt tagagcaaca atatctggag tccttagagg tttattaaag gatggagcaa
    78181 aggtaaacaa aaatttaaaa atactagaca tagaccctag aaaagaagaa gttaagaatg
    78241 cttttacaat atcagataaa gctagatgta tagctggagg ggttttagaa gccatactaa
    78301 acaatgggat ttatccccaa aattaattta tattagataa agctattaat attttattaa
    78361 gatgatataa taaatttaat agattaaaga gagcatatga cgggtattta aaatttaaat
    78421 ggtaaaaatg ctcttttttt attggacatt caattaacat gtgtatataa tataaaaata
    78481 agtatttatt taggttgaaa tctaataaat atataatacc agtacataag caaagaggag
    78541 aaaagtaaat tatgagacca tcatgggatg aatacttcat ggaaattgca gaagtagtga
    78601 aaaagcgttc aacttgtatt agaagacaag taggagctgt aattgtaaga gacaaacaaa
    78661 tattaacaac tggatataat ggttctccga gaaatcttga acactgtgaa aatattggat
    78721 gtaagaggca ggaattaaat attccctcag gagaaaggca tgaattatgt agagcattac
    78781 atgctgaaca aaatgcgata atacaagctg ctcataatgg tattagtgta gatggggcaa
    78841 ctttgtatgt tactacaaga ccttgtgttt tgtgtgcaaa aatgtgcata aatgcaggta
    78901 tattgaaaat tgtttatgaa ggtgattatc cagatgatat gtctacagaa ttattaaaag
    78961 aagcaggaat agaactcata aaattttagt ggataaaatt tatatgacaa taaatgtcaa
    79021 attatgacac tgaaatacaa tggtatcaaa tgattaaaag aaagtatttg aaaaatcatc
    79081 agattagtag taagatatat tttataatta aataaattta tgagatagat taattacatg
    79141 ttatttattt tataataatg agggatacta tttatgagta aaagaaatac acatacagaa
    79201 attgcaaagt tgtttagttt aataagtcaa ataggattga tgattgtaat atcaatatta
    79261 ggatgtactt tcttaggaaa atttttagat agtaaattga atactactcc agttctgaca
    79321 ataatatttt tattgttggg tgttggaggt gcttttagtg gtgtttataa aactctaata
    79381 gtatatacac gaaggaagtg atatattgga tataaaatta cttaaccagg taaggtcagt
    79441 aacaaaaggt atagtaatat ttgatgtagt tttaattgtg ctaatgttga taacatcaac
    79501 attaagtaaa ccagtattat taggacttat atttggctct ataatagcta tgttaaattt
    79561 tagattattg gcgatatcac tggaaaaatc agtgacattt cctgctggga aggcacaagc
    79621 ttatgcatca gcacaatatg ctgttagatt atttataatg gcagttgttc taattgcatc
    79681 agctacagta ccacatttaa atataatcgg aacagcattg ggattattga gcacaaagtt
    79741 tgtcatatta tcaaaaaact ttataaataa attaaagagg aaggaggcat aagtatgaat
    79801 caagcgcatt attatctcat aggaaacttt aagattagtg aaacaatact agtaagttgg
    79861 ggaataatgg cctttttagc gatagcatct tattttttga ctagaaactt aaaaaaagtt
    79921 ccaactagta aagttcaaat attcttagaa tttgcagtag gtggtcttgc taaaatggtt
    79981 acagatgtta tgggtaaaaa tacagtaaaa agaatgccat atatgatacc atacatagga
    80041 agcttgtttt tatttttcgc atgttcaaat ttaagcccat tacttggatt aaggtcacca
    80101 acaaccgact tagacacaac tgctgcatgg gcaatgatta cattttttat gatttatttt
    80161 gcaggagtta aatttaaggg gttatcatac tttaaagaat tggctcaacc acttgccata
    80221 atgttaccaa tcaatattat aggtgagtta gctagaccaa tatcattaag tttccgtcca
    80281 tttgggaata ttttaggtgg aacaataata atgggtctgt tttatcaact tttaggtttt
    80341 atatctagtt taataccagg attatcaata ccaattggag aaatattaat accagtccca
    80401 ttacacttgt actttgactt atttgcagga ttgttacaag catatatatt tataatgtta
    80461 acaatggtat ttatatcaag tgcaggagag ttagattaag aaatcaactt atattaagat
    80521 aaaaataaaa taatttaaaa aattaaagtt ttaaggagga aataattatg gaaacagcaa
    80581 tagtagcggc agcatcagca ataggagcag ggatagcagt tgcaacaggt ataggtgcag
    80641 gtataggtca aggtatagca gcagctaaag ctgcagaagc agtaggaaac caaccagaag
    80701 ctaaaggtga tataacatca actttattac taggagttgc tatagcagag tcttcagcaa
    80761 tatatggttt ggttatatca ataatacttc tattcgtaaa tccattcttt aaatacttag
    80821 gaatgtagat ttagtcctta aatattttga gataggttta taggtaaatc atctcaaact
    80881 tataatcatg agatgaactt tatcatctcg tgattataag tagttatgat ttatggaaag
    80941 gaggataatt atggaaaaag cactagtagg tataacgtgg gaatttgtgt tccaaattgt
    81001 taatactttt ataatatttt tacttctgag aaagctttta ttcaagccag tattaaatat
    81061 aattgaatca agagaaaatg atattaaaag tgatttggca gaaggcgaaa aagcaaaaaa
    81121 tgaaggttta gctctgaaaa aagaatatga gtcaaaaatc aattttgcca aggatgaagg
    81181 tcaagaaatc ataaaacaag caactataag agcagagcag aaatcagatg atatagtaaa
    81241 tactgcaaag aaagatgctc ttgatataaa agaaaaagca aacaaagata tagaacaaga
    81301 aagacaaaag gttataaatg aaatcaaaaa tgatatatct aacatagcac tacttgcagc
    81361 atctaaagtc attgaaaaag atttagataa atctaaacat gaagaattga tagaaaactt
    81421 tataaaagag gtaggggaag ctaaatgata aatgtaatag caaatagata tgcagaagcc
    81481 ctatttcaat taggagaaga agaaaattca actgatgtct tattcaaaga actagaaaaa
    81541 gtagtagata tgatgactaa agttagtaaa gatttctaca aagttttaaa atcaccatta
    81601 gtttcaaaat cagaaaagaa aaacttggta gaaattattt ttagtaaaga agtaagttct
    81661 aacataaaaa actttttaaa agttttagta gataaagata gaatatcata tttagaagat
    81721 atcgaattag cttataaaga attattaaat aagaaaaaca atgtaattga tggagtagca
    81781 ataagtgcta ttccaatgag tgaaacagat attaaagaac ttgaagtcaa actttcaaat
    81841 aaatacaaca aaaatgttac tatagaaaat gttgttgaca agactatact agggggagtt
    81901 ctagttagaa ttggaaatga acaaatagat ggcactgtga aaactcgtct tgataagatg
    81961 aaagaaaagc tatctgaagt aatatcttaa gaatggcggt gaacccatga acttaaaacc
    82021 tgaagaaata agttctataa ttaaacagca aataaaaaat tatgagaata aagttgagtt
    82081 gacagataca ggtagtgttt taacagttgg ggatggtata gcgagtgtat atggattaga
    82141 aaaagctatg tctggtgagt tgctagagtt cccaggtgaa atatatggta tggcacttaa
    82201 cttggaagaa gaagtagttg gagctgttat ccttggagat gacagtgaaa taaaagaagg
    82261 cgatatagtt aagagaactg gaagaatagt tgaagttcca gttggagaag ctttaatagg
    82321 tagagttgta aactcactag gtcagccaat agatggaaaa ggacctatag cgtacacaaa
    82381 aactagacca gttgaatctg aggcacctgg tataatagat agaaggtctg tttatgaacc
    82441 attacaaaca ggaatcaaat caatagattc aatgatacca attggtagag gtcaaagaga
    82501 gttaataata ggtgacagac aaactggtaa aacatctata gttattgaca ctatactaaa
    82561 tcaaaaagga aaagatgtta tctgtatata tgtagcaatt ggacaaaaac gttctactat
    82621 agctcaatta gttagctctt tagaaaaagg tggagctttg gattatacaa tagtagtttc
    82681 agcaacagca tctgaatcag caccacttca atatatagca ccatatgctg gagcagctat
    82741 gggtgaagaa tttatgtaca atgggaaaca tgtacttata gtgtatgatg atttaagtaa
    82801 acaagctgtt gcatatagag aaatgtcatt gttacttaga agaccaccag gacgtgaggc
    82861 atatcctgga gatgtattct atttacattc aagattactt gaaagagcag ctaagttatc
    82921 tgatgaattg ggtggaggtt caatgactgc cttgcctata atagaaactc aagctggtga
    82981 cgtttctgca tatataccaa caaatgttat atctataaca gatggtcaaa tatacttaca
    83041 accagagtta ttctattcag gtgtaagacc agcagttgac cctggtatat cagtatcaag
    83101 ggttggtggt tctgcgcaaa ttaaagcaat gaaaaaagtt gcaggaacat taaaacttgc
    83161 atattcacaa tatagagaac ttgcagcatt ctcacagttt ggttctgact tagatgaaga
    83221 tactaaaaag agacttgctc aaggtgaaag aatcgttgag atattaaaac aaggtgagca
    83281 tcaaccaata aaagttgaaa atcaagttat gataatatat gctgttataa ataatcattt
    83341 agaggatatt ccgatagata atatagctag atttgaatca gaattgtatg catttgtaga
    83401 taataattat ccggaaatat caagaaaaat attaggtgga gaagacttca ctcatgattt
    83461 aacagatgca ataaatgaat ttaaagaaaa gtttgttgtt gaagtataat cctttttaaa
    83521 aaaggaggta aattaattgg caggagctgg aatcaaagca atcaaaacac gtatgggaac
    83581 tgttgaaagt acaaagcaaa taactaaagc aatggaatta gttgcatctt ctaagcttag
    83641 aaaagcaaaa gaaaaggctg aagattcaag agaatatttt aatactttat acaatacagt
    83701 aaaagatatt gcccaaacaa cacgtggaat tagaaatgaa tttttaaaag aaagagaagt
    83761 aaaaaataaa tgctatgtag tagtagctgg agacagagga cttgcaggtg gatacaattc
    83821 taatgtatta aaggaatctg tctctcatat gaaaggtaaa aaggaaacag tgattactgt
    83881 tggaaagaaa gcacatgaat ttttttctaa aagaaactac aatatagcaa aagatataaa
    83941 tagtgttgaa gcttgtggat atgaagatgc gacagaaatt tcacaaacta ttattgacct
    84001 gtataaaaaa ggtgaagtag atgaagttta tattgcttat acaaaattta aatctgcttt
    84061 atcacaagaa gttgaaattt tgaaattgct tccattagtc tttgaaaaag atacagacga
    84121 acaaggaatg gcagtaaaag aagaaaaaca aaatagatca aaagtacaat atataccatc
    84181 agcagaagca gttttaagtt atattgtacc tcagtttata tcaggtacag tatatggtgg
    84241 aattttggaa tcttttgcat cagagcaagg tgctagaaga actgcaatgg aatcagccac
    84301 agacaatgcg aatgaaatgc tatctaattt agagttaagt tataacagag caagacagtc
    84361 agctgtaact caagaaataa cagagatagt tggtggagtt gaagctctaa aatagggagg
    84421 ttaggaaatg gcaaatgtag gtaaagtagt acagattgta ggagctgtac tagatgttaa
    84481 gtttgatagt gaacaaagcc tacctaactt attaaatgcg ttagtaataa agttaggaga
    84541 taaagaaata gttgcagaag ttgctcaaca tataggtgat gatactgtaa gatgtatagc
    84601 tatgagtgca acagacggac ttgtaagagg tatggaagtt gtagatacag gagggccaat
    84661 aagtgttcct gttggagatg aaactttagg tagaatattc aatgttcttg gaaaaccagt
    84721 tgatgggaaa ccagctccaa aatctgcacc taaattacct atacatagac cagcaccagc
    84781 atatgatgaa ctagaaacta cagcagagat actagaaact ggtattaagg tagttgactt
    84841 actagctcca tatctaaaag gtggaaaaat aggtctattt ggaggagcag gagtaggaaa
    84901 gacagttcta atacaagagc ttataaacaa tattgctaag caacatggtg gtatttctgt
    84961 gttttcagga gtaggagaaa gaacaagaga aggtaacgac ctttatggcg aaatgagtga
    85021 gtctggagtt ataaataaaa cagctctagt atttggtcaa atgaatgaac cacctggagc
    85081 gagaatgaga gttgctttaa ctggacttac aatggcagaa cattttagag atgagcaagg
    85141 acaagacgtt ttacttttcg ttgataatat attccgtttc acacaagctg gttcagaagt
    85201 ttcagcactt ctaggacgta tgccatcagc tgttggttat cagccaacat tagctactga
    85261 aatgggtgca cttcaagaga gaataacatc aactaagaaa ggttcaataa catctgttca
    85321 agcagtatat gtaccagcag atgacttaac tgacccagca ccagctacaa cattctctca
    85381 cttagatgca aaaacagttt tatcaagaca aatatcatca ctgggtatat atccagcagt
    85441 tgaccctctt gaatctacat caaggattct tgaccctagt atagtaggaa aagaacatta
    85501 tgaagtagct agaggtgtac aatcaatact tcaaagatat aaagaacttc aagatataat
    85561 tgctatactt ggtatggatg aattatctga tgaagataag ttaatagttg cacgtgctag
    85621 aaagatacaa agattcttat ctcaatcatt cacagttgct gagcaattta caggaaatcc
    85681 aggtcaatat gttcctgtta aagagactgt aagaggattt aaagaaattc ttgaagggaa
    85741 acatgatgac ttaccagagt cagcattctt atttgtagga acaatagaag acgctgttag
    85801 aaaagcgaag ggaagtatgt agtcatggcc agtgaatttt cattgaaagt tgtaactcca
    85861 agtaatactt tttatgatgg agatgttgaa atgatagtag ttagaactac aaaaggagac
    85921 aaaggggtgt tgaaaggtca tataccattt gtttcaggta tacctgtagg aactttaaag
    85981 ataaaaaaag agggaagttt taaagaggcg aaaatagcag aaggattcat taatgtaact
    86041 caagataaaa ctgtcatatt gacagaatct gctgagtgga tttaataaat taatttagag
    86101 tataaaaagt cattttgacc aaaaaaactt ttgggtaaaa tggctttttt gcgttttggt
    86161 gttaataaaa taacgataaa attgtaagtt tttagtgata ataaattcac aaaatgttta
    86221 aatttactgc caaattatgt tgtaattaat aagataaaga agatttagat agcttttatg
    86281 taagtgaaaa agtcttcata taagaaatac atataaaatt tttataaaat ttaa
//
LOCUS       DAYATN010000002        84143 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.002, whole genome shotgun sequence.
ACCESSION   DAYATN010000002 DAYATN010000000
VERSION     DAYATN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 84143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.002"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            374..3028
                     /gene="adhE"
                     /locus_tag="ACGI8A_000088"
                     /gene_synonym="adhC"
     CDS             374..3028
                     /gene="adhE"
                     /locus_tag="ACGI8A_000088"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFH0178104.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            3304..5523
                     /locus_tag="ACGI8A_000089"
     CDS             3304..5523
                     /locus_tag="ACGI8A_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0178105.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            5777..6439
                     /locus_tag="ACGI8A_000090"
     CDS             5777..6439
                     /locus_tag="ACGI8A_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFH0178106.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            6810..8219
                     /locus_tag="ACGI8A_000091"
     CDS             6810..8219
                     /locus_tag="ACGI8A_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFH0178107.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            8334..8609
                     /locus_tag="ACGI8A_000092"
     CDS             8334..8609
                     /locus_tag="ACGI8A_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HFH0178108.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            9239..9550
                     /locus_tag="ACGI8A_000093"
     CDS             9239..9550
                     /locus_tag="ACGI8A_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178109.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            9552..11477
                     /locus_tag="ACGI8A_000094"
     CDS             9552..11477
                     /locus_tag="ACGI8A_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFH0178110.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            11480..11971
                     /locus_tag="ACGI8A_000095"
     CDS             11480..11971
                     /locus_tag="ACGI8A_000095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFH0178111.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            12023..12586
                     /locus_tag="ACGI8A_000096"
     CDS             12023..12586
                     /locus_tag="ACGI8A_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFH0178112.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            12601..13578
                     /locus_tag="ACGI8A_000097"
     CDS             12601..13578
                     /locus_tag="ACGI8A_000097"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HFH0178113.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            13571..13888
                     /locus_tag="ACGI8A_000098"
     CDS             13571..13888
                     /locus_tag="ACGI8A_000098"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFH0178114.1"
                     /translation="MYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVEDEY
                     AIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGADIL
                     FKE"
     gene            14005..15783
                     /locus_tag="ACGI8A_000099"
     CDS             14005..15783
                     /locus_tag="ACGI8A_000099"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFH0178115.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            15780..17153
                     /locus_tag="ACGI8A_000100"
     CDS             15780..17153
                     /locus_tag="ACGI8A_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFH0178116.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            17159..17827
                     /locus_tag="ACGI8A_000101"
     CDS             17159..17827
                     /locus_tag="ACGI8A_000101"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFH0178117.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            18104..19153
                     /locus_tag="ACGI8A_000102"
     CDS             18104..19153
                     /locus_tag="ACGI8A_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0178118.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            19249..20238
                     /locus_tag="ACGI8A_000103"
     CDS             19249..20238
                     /locus_tag="ACGI8A_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178119.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKGKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            20243..21961
                     /locus_tag="ACGI8A_000104"
     CDS             20243..21961
                     /locus_tag="ACGI8A_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HFH0178120.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(22043..23197)
                     /locus_tag="ACGI8A_000105"
     CDS             complement(22043..23197)
                     /locus_tag="ACGI8A_000105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFH0178121.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            23696..25057
                     /locus_tag="ACGI8A_000106"
     CDS             23696..25057
                     /locus_tag="ACGI8A_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0178122.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            25148..25852
                     /locus_tag="ACGI8A_000107"
     CDS             25148..25852
                     /locus_tag="ACGI8A_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0178123.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDVRVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            26052..26363
                     /locus_tag="ACGI8A_000108"
     CDS             26052..26363
                     /locus_tag="ACGI8A_000108"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0178124.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            26385..27698
                     /locus_tag="ACGI8A_000109"
     CDS             26385..27698
                     /locus_tag="ACGI8A_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0178125.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            27795..29105
                     /locus_tag="ACGI8A_000110"
     CDS             27795..29105
                     /locus_tag="ACGI8A_000110"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFH0178126.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            29131..29862
                     /gene="chbG"
                     /locus_tag="ACGI8A_000111"
     CDS             29131..29862
                     /gene="chbG"
                     /locus_tag="ACGI8A_000111"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFH0178127.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLLSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            29875..30192
                     /locus_tag="ACGI8A_000112"
     CDS             29875..30192
                     /locus_tag="ACGI8A_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFH0178128.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(30304..30768)
                     /locus_tag="ACGI8A_000113"
     CDS             complement(30304..30768)
                     /locus_tag="ACGI8A_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178129.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            31038..31976
                     /locus_tag="ACGI8A_000114"
     CDS             31038..31976
                     /locus_tag="ACGI8A_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0178130.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLAKPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            32104..33117
                     /locus_tag="ACGI8A_000115"
     CDS             32104..33117
                     /locus_tag="ACGI8A_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFH0178131.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            33123..34145
                     /locus_tag="ACGI8A_000116"
     CDS             33123..34145
                     /locus_tag="ACGI8A_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFH0178132.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            34160..34942
                     /locus_tag="ACGI8A_000117"
     CDS             34160..34942
                     /locus_tag="ACGI8A_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178133.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            34954..36294
                     /locus_tag="ACGI8A_000118"
     CDS             34954..36294
                     /locus_tag="ACGI8A_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0178134.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(36389..38668)
                     /locus_tag="ACGI8A_000119"
     CDS             complement(36389..38668)
                     /locus_tag="ACGI8A_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0178135.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(39005..40168)
                     /locus_tag="ACGI8A_000120"
     CDS             complement(39005..40168)
                     /locus_tag="ACGI8A_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFH0178136.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VNYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            complement(40181..40456)
                     /locus_tag="ACGI8A_000121"
     CDS             complement(40181..40456)
                     /locus_tag="ACGI8A_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFH0178137.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            complement(40477..41451)
                     /locus_tag="ACGI8A_000122"
     CDS             complement(40477..41451)
                     /locus_tag="ACGI8A_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HFH0178138.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            complement(41722..43635)
                     /locus_tag="ACGI8A_000123"
     CDS             complement(41722..43635)
                     /locus_tag="ACGI8A_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0178139.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            complement(43812..45026)
                     /locus_tag="ACGI8A_000124"
     CDS             complement(43812..45026)
                     /locus_tag="ACGI8A_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0178140.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            45270..46652
                     /locus_tag="ACGI8A_000125"
     CDS             45270..46652
                     /locus_tag="ACGI8A_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HFH0178141.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIESGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            46664..48013
                     /locus_tag="ACGI8A_000126"
     CDS             46664..48013
                     /locus_tag="ACGI8A_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HFH0178142.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSITSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            48163..48696
                     /locus_tag="ACGI8A_000127"
     CDS             48163..48696
                     /locus_tag="ACGI8A_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFH0178143.1"
                     /translation="MSGAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            48780..49589
                     /locus_tag="ACGI8A_000128"
     CDS             48780..49589
                     /locus_tag="ACGI8A_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0178144.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            50000..52045
                     /locus_tag="ACGI8A_000129"
     CDS             50000..52045
                     /locus_tag="ACGI8A_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0178145.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            52306..53505
                     /locus_tag="ACGI8A_000130"
     CDS             52306..53505
                     /locus_tag="ACGI8A_000130"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HFH0178146.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            53557..54252
                     /locus_tag="ACGI8A_000131"
     CDS             53557..54252
                     /locus_tag="ACGI8A_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HFH0178147.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            54265..54942
                     /locus_tag="ACGI8A_000132"
     CDS             54265..54942
                     /locus_tag="ACGI8A_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178148.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            55053..56654
                     /locus_tag="ACGI8A_000133"
     CDS             55053..56654
                     /locus_tag="ACGI8A_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0178149.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCYGKIITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            57103..58392
                     /locus_tag="ACGI8A_000134"
     CDS             57103..58392
                     /locus_tag="ACGI8A_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFH0178150.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            58421..59101
                     /locus_tag="ACGI8A_000135"
     CDS             58421..59101
                     /locus_tag="ACGI8A_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFH0178151.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            59202..59741
                     /locus_tag="ACGI8A_000136"
     CDS             59202..59741
                     /locus_tag="ACGI8A_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HFH0178152.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            59917..60936
                     /locus_tag="ACGI8A_000137"
     CDS             59917..60936
                     /locus_tag="ACGI8A_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFH0178153.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            61224..62414
                     /gene="dltD"
                     /locus_tag="ACGI8A_000138"
     CDS             61224..62414
                     /gene="dltD"
                     /locus_tag="ACGI8A_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFH0178154.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            62535..64049
                     /locus_tag="ACGI8A_000139"
     CDS             62535..64049
                     /locus_tag="ACGI8A_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HFH0178155.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDEKDFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            64049..65197
                     /gene="dltB"
                     /locus_tag="ACGI8A_000140"
     CDS             64049..65197
                     /gene="dltB"
                     /locus_tag="ACGI8A_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFH0178156.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            65216..65446
                     /gene="dltC"
                     /locus_tag="ACGI8A_000141"
     CDS             65216..65446
                     /gene="dltC"
                     /locus_tag="ACGI8A_000141"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFH0178157.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            65663..66448
                     /locus_tag="ACGI8A_000142"
     CDS             65663..66448
                     /locus_tag="ACGI8A_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFH0178158.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            66727..68637
                     /gene="phnW"
                     /locus_tag="ACGI8A_000143"
     CDS             66727..68637
                     /gene="phnW"
                     /locus_tag="ACGI8A_000143"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFH0178159.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            68996..69553
                     /locus_tag="ACGI8A_000144"
     CDS             68996..69553
                     /locus_tag="ACGI8A_000144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178160.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHISSR"
     gene            69646..69858
                     /locus_tag="ACGI8A_000145"
     CDS             69646..69858
                     /locus_tag="ACGI8A_000145"
                     /inference="COORDINATES: protein motif:HMM:NF024570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HFH0178161.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLD"
     gene            70424..70831
                     /gene="tnpA"
                     /locus_tag="ACGI8A_000146"
     CDS             70424..70831
                     /gene="tnpA"
                     /locus_tag="ACGI8A_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFH0178162.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            70833..71951
                     /gene="tnpB"
                     /locus_tag="ACGI8A_000147"
     CDS             70833..71951
                     /gene="tnpB"
                     /locus_tag="ACGI8A_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFH0178163.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQYIKLPKLGMVKVRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTNVDIEVFENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFVRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEMKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            72072..72479
                     /locus_tag="ACGI8A_000148"
     CDS             72072..72479
                     /locus_tag="ACGI8A_000148"
                     /inference="COORDINATES: protein motif:HMM:NF024570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HFH0178164.1"
                     /translation="MANMFMKKDINKEFDIDFILSKEVICNYTKEIFQNLYKKEQSIG
                     KKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKIYLFRKFGVPFVVG
                     CLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(72779..73366)
                     /gene="rbr"
                     /locus_tag="ACGI8A_000149"
     CDS             complement(72779..73366)
                     /gene="rbr"
                     /locus_tag="ACGI8A_000149"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HFH0178165.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(73452..73889)
                     /locus_tag="ACGI8A_000150"
     CDS             complement(73452..73889)
                     /locus_tag="ACGI8A_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0178166.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            73993..74991
                     /locus_tag="ACGI8A_000151"
     CDS             73993..74991
                     /locus_tag="ACGI8A_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HFH0178167.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(75111..76037)
                     /locus_tag="ACGI8A_000152"
     CDS             complement(75111..76037)
                     /locus_tag="ACGI8A_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFH0178168.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            76422..78026
                     /locus_tag="ACGI8A_000153"
     CDS             76422..78026
                     /locus_tag="ACGI8A_000153"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFH0178169.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            78842..80113
                     /gene="serS"
                     /locus_tag="ACGI8A_000154"
     CDS             78842..80113
                     /gene="serS"
                     /locus_tag="ACGI8A_000154"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFH0178170.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            80391..81896
                     /locus_tag="ACGI8A_000155"
     CDS             80391..81896
                     /locus_tag="ACGI8A_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFH0178171.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            82010..82708
                     /locus_tag="ACGI8A_000156"
     CDS             82010..82708
                     /locus_tag="ACGI8A_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0178172.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            82993..84081
                     /locus_tag="ACGI8A_000157"
     CDS             82993..84081
                     /locus_tag="ACGI8A_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178173.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT        32055 a         9663 c        14562 g        27863 t
ORIGIN      
        1 actatattta ctttaacttg ttaattaagt tttaattttc attttcaagg atttttattt
       61 ttaaacttga tagagtatca tattaaaaat tctttttata aagttttatt tgcaaaaaat
      121 acaaaaatat aaacgttaaa aaaataacgt tatccttaag tttttagatg atttcattag
      181 tgatttgcgt aaaaaataaa gtgatatcta gttaaattaa gtatttgtaa agctagatat
      241 tattagtaga aatgatgtta tattagataa aaaataaaat gttaaaaaaa taacttgaaa
      301 ataggaaaag taaatgctat aatgttaata aattaacata ataaataaaa aactatctca
      361 ggaggttgtt aaaatggctc ataaagaaca agtaataata gaaaataaaa aagaaaatga
      421 agttatgtca gttggtttag atactgtaaa taacgttgaa agcttagtta aaaaactagc
      481 aaaaatcaga gaagctcaaa aaatatttgc tacatatagt caagagcagg tagataaaat
      541 ttttttagca gcatctttgg cagctaataa gcaaagagtt cctctagcta taatggcaca
      601 aaaagaaact ggtatgggaa ttgctgaaga taaggttata aagaatcatt atgcctcaga
      661 gtatatatat aatgcatata aagaaactaa aacttgtggt gttatagaga aagacgaagc
      721 atttggtatg actaaaattg ctgaaccaat aggtgttatt gcagcagttg tacctacaac
      781 taatccaact tctactgcta tctttaaagc acttttagct ttaaaaacta gaaatggaat
      841 aattttctct ccacatccaa gagcaaaaaa ttctactata gaggctgcaa aagttgttct
      901 tgaggctgct gttttagctg gagctcctga aggtataata ggatggatag atgagccatc
      961 tttagaatta actacaactg ttatgaaaga agtggatttg acccttgcta caggaggtcc
     1021 tggaatggtt aaatcagcat attcatctgg aaaaccagct attggagttg gtgcaggtaa
     1081 tactccagca ataattgatg atagtgcaga tataaaaact gctgtaaact caattcttgt
     1141 atctaagact tttgacaatg gtatgatatg tgcttctgaa caatcggtta tagtattaga
     1201 aaatatatac aatgaagtca aaaaagaatt taaagagcgt ggtgcttatt tattagataa
     1261 agatgaaaca gaaaaaataa gaaatataat tttagtaaat ggaggattaa attctaaaat
     1321 agttgggcaa acagcttgta ctatagcaaa acttgcaggc tttgaagtcc cagtagatac
     1381 taaggtttta ataggtgaag tagaatcagt tgaaatagaa gaagcttttg ctcatgagaa
     1441 actttcacca gtacttgcaa tgtacaaggc aagtgatttt gatgatgcag ttagaaaagc
     1501 agaaaaatta gtagaagatg gtggatttgg gcatacatct tcattatata ttgatgatgt
     1561 aaatcaaaga gaaaaacttg ataagtttac ttcagccatg aaaacttgta gaattttaat
     1621 taatactcct tcttctcaag gtggtatagg ggacttatat aattttaaac tagctccttc
     1681 tcttacttta ggatgtgggt cttggggtgg aaattcagta tctgaaaatg taggagttaa
     1741 gcatttactt aatattaaaa cagtagctga gaggagagaa aatatgcttt ggtttagagc
     1801 accagaaaaa gtttatttca aaaaaggttg tttaggagta gcattaaaag aacttaaaga
     1861 tgtaatgaat aagaagagag cttttattgt aacagataca ttcctttaca ataatggata
     1921 tactaaagca gttactgatt tattagatga aatgaatatt aagtacacta cattctttga
     1981 agtagaacca gacccaactt tggaatgtgc taagataggc gctaaagcta tgagagaatt
     2041 taatccagat gtaataatat ctataggtgg aggaagtgca atggatgcag gaaagataat
     2101 gtggacattg tatgagcatc cagatgtaga tttccaagac ctagctatga gatttatgga
     2161 tataagaaaa agggtatata cattccctaa aatgggtgaa aaagctaatt ttgtagctat
     2221 accaacatca gcaggtactg gttcagaagt aacaccattt gctgttataa ctgaccaaga
     2281 tacaggagta aaatatccat tagcagatta tgaactgatg ccaaatatgg ctatagttga
     2341 ttctgatatg atgatgaata tgccaaaaag cttaacatct gcttctggta tagatgcttt
     2401 aactcatgca ttagaagcat atgtatctat gcttgcaacc gattatacaa atggattagc
     2461 tttacaagct attaagagta tatttgaata tcttccacgt gcatatgata atggtgcaaa
     2521 agacccagaa gctagagaaa aaatggcaaa tgcttctaca atggcaggta tggcttttgc
     2581 aaatgcattt ttgggtgttt gtcactctat ggctcataaa ttaggtgctt tccaccatgt
     2641 accacatggt gttgcaaatg ctcttttgat aacagaagtt atgaaattta attcttcaga
     2701 tgcaccaaag aaaatgggag cattctctca atataaatat ccagaagcat taaagagata
     2761 tgctgaaata gcttcatttt taggattaaa aggaaattct gatgaagaga aattccaaag
     2821 tttattggta gctattgaag atttaaaact taaagttggt attcctaaat ctataaaaga
     2881 gtttggcgtt gaagaatcta agtttatgga ttcaatagat gaaatggtaa ttcaagcatt
     2941 tgatgaccaa tgtacaggag caaatccaag atatccactt atgaatgaaa taaaagatat
     3001 gtatttaaat tcatattatg gaagataagt ttgctataaa atagataaaa taaaaactct
     3061 tatacataaa acaaagtttt attgtataag agtttttttg tttgagaaaa tggatagttt
     3121 ttattcttta atttaaaatt caattaaata gtgttattat aataataaaa gaatgttttt
     3181 atggttatta tgttgatttt atgtgtaaca tttaagaata gttatataca atataaatga
     3241 tgaattggta taaaaaggat atattatcta tatgaagtat tgactaatta ggggaggagt
     3301 cgaatgacag ggagaaataa aagttttaca ttaattacaa ttcttttttt aacatcatta
     3361 tttttgacaa tggggtatat ctatatagaa gatacaaaac atcttcttat gtcagaggca
     3421 aaaatacata taaaagaagt tgcagtacaa ggctctcaac tagcacaaag acaaattgaa
     3481 aaagatttag atgttttaaa tattttttct aactattatg cttcaaatcc agatatacct
     3541 aatgaggaaa aaatgaaaaa tcttttagat gaaatggaaa atcaaaaatt ttacactatg
     3601 gcaatagttg atataaatgg taatgctgaa aataccaaag gtgataagtt ttcagtgaaa
     3661 gatagagaat ttttcaaaaa ctcaattaaa ggtaagaagt atgtttcaag tccatatgtt
     3721 gatgaagtaa ataagtctat aaagaagatt gctatttcag taccattatt aaataatgat
     3781 aaagtagttg gagttttata ttgtacttac aatatcaata ctctgatgaa acttattaat
     3841 atatcttttt atgaaaataa tagtatatct tatgttgtaa aaaataatgg tacaattatc
     3901 cttcatccac aaggggatag cctatcaaaa aatatttata aattgttaaa acaggataat
     3961 gatattcaag aagtaaatag attaaaaaaa gaattgcaag aaaataaaac aggtgcaact
     4021 gtattaaata tgttggaaga aagaagatat ttaggatatg ccactatgga taatgggaat
     4081 agtgaaaata attatataaa agactggaat ttaatttttt caattccaga aactgttgta
     4141 tttagtaatt caaaacaaat aatcaacaga gctgtatatg cagtgttatc tattgtatta
     4201 atatttattg caattatttt ttatataatt tatataaaaa aatctaatga aaaagaaata
     4261 ttaagtttgg cttatgaaga taaagttaca tatataggaa atcaaaataa attttataga
     4321 gaatgtagta agtatctttt agataaacca tctttaaatt atattatagt atattttgat
     4381 attaataact ttaagatgat taatgatact tttggatatg aatttggtga caatttatta
     4441 attacaatag caaaagcttt aaaagaagaa ttaacagaag gtgaggtata tgcacgactt
     4501 tcaagtgatt attttgcaat tttctgtgat tataaaaatg gaagaaataa aattataaga
     4561 aaacttgata atatacgaag taatattgaa agtaatttga gtatagtctt tgaaatttct
     4621 ctgtgtgtgg gtatttattt tgttgaagaa ggtgaagtag atattcaaaa ggctgtaaat
     4681 aaagcaaata tggctaggtc tgttgctaaa ggtaaaaata taaattatgc tatttataat
     4741 gaagatgtta ggaataagct tagtgaggaa tcaatgattt tagatgatat aaaaatagca
     4801 ttggtaaaaa atcaatttga agtgtattat caacctaagt tttcacttgt aactggagaa
     4861 atgataggga gtgaagcact aataagatgg aatcatccag aacatggttt tataagtccc
     4921 gcagtattta ttccaatagc agagaaaagt aagttgattt taaaaattgg gagatttgta
     4981 tttgaaaggg tttgcaatga tttatatgaa tggaaaaagc aaggtaaaaa gatagttcca
     5041 gtttcagtaa atttatctag ggtcgagttg tatcaaccag atattgtaaa atttatcaat
     5101 aaaacaataa aaatgtataa tttaagttca gatttcattg aaatagagat aacagaaact
     5161 gtggctatta atgagttaaa tattttaaag aatgttttaa atgagttgag gacgtatgga
     5221 ttttctattt ctatggatga ctttggtaca ggatattctt ctattagttg tttaagagat
     5281 atgccaattg atatattgaa gctagataag tcttttcttg gtggcattga acatgatgag
     5341 agaagtcgca atatagcaaa atcaatagtg tctctagcaa aatcactaga tttagttgta
     5401 attatagaag gtgttgagag taaagaacaa gctgagttaa tgaaacaatt tggatgtgat
     5461 ttagttcaag gattttattt tgcacgacca atgccagcta agaatttttt agatttactt
     5521 taaaacaaaa tttgattttt atataaagca ctattatgaa tgcttgattt taataaatca
     5581 ttgctaaata attagtgttt ttttatttta tcaatgtatt gtctaatata aagcaaaaaa
     5641 taattgtatt ttataaaaat tatattaaaa ataaatttat tttgctaata aacatgttat
     5701 taacaaaaga atagtactat aatgtataga tgataaaaac tttaataaaa atttagtgta
     5761 taggagttgt ttaagtatga tagatataag tattgatgac aaacttaagg aaagatggcc
     5821 taatgctaag ctgggttgca ttcaagcaaa agtagcagta aataaaagct cagaaaaatt
     5881 aattaatgaa ataaatgagt tttgtgatac tataaatcaa agtctaaaag ttgaagacat
     5941 tacgaaacta gataaaatta aagatgcaag aaatgcttat aaagagcttg gaaaaaatcc
     6001 aagtagatat agaacatctt cagaagcatt ggttagaaga attattcaag ggaagggtct
     6061 ttatacaata aataatatag tagaaataaa caatttaata tcaataaaat cattatatcc
     6121 agtaggtaca tataatgtaa gcaaattaca ttcaccagta tgttttactg taggagatga
     6181 aggagaacag tataagggta ttgggaaaga attaataaat atagaaaatc ttcctatatt
     6241 atctgattca gtgggtaaat ttgggagtcc aactagtgat tctgaaagag caatgataac
     6301 agaagatgca aatgaaatat taatttgtat attttctttt agtcaagaat ctgatattga
     6361 agaatatttt ggatatgcta aggaattact taaagaatat gcagatggaa aagatataga
     6421 aactaaaata attaaataga atctatataa caacgttatg ttaagctaaa attcaatata
     6481 aaaataagtt aagataaaga aactaacaca aaattagttt cttttttaaa atagtagttt
     6541 agtgttacaa aaatagatag atagtcccac gattattaaa agtatggtaa tatattaata
     6601 tagcagagat tgtgtttaag ggattttgaa aggggggatt tataataatc attttatcta
     6661 aaagataaaa tggttttata gaaataacta tatatttaaa aatataaata atgatacagt
     6721 aatttgggta tataaaataa ggcatacatg gttttgttta aaaaccaata aggtacatag
     6781 gtaattacaa tgtaaggaag gatttaatta tgattgatgg aaaagaggta aaacaagaaa
     6841 gtggacaaaa aaatctaaaa tttaagatag ctatagtagt aggtgttttg tttgtagctt
     6901 atataggaat ggcaatcttt tttagaaatc atttttactt tggaacgact attaatggcg
     6961 taaaggcttc aggaaaaaca gttgagcaag taaatgattt gttaacatct agtactaatt
     7021 cctatacttt aaacttagag gaaagaaaca ataagaaaga acaaataaaa gcaaaagata
     7081 ttgatttaaa atttacacca gatgataaag ttcaaaagat aaaagatagt cagagcgcta
     7141 ttggatggat tggtggaata tttaagtcta aagagtataa taatatgata actctttcat
     7201 ataataaaga tttactagaa aaaaggttta atgctttatc ttgttttgat agtaaaaatg
     7261 ttgtagaacc taagagtgct tatcctaagt atgatagcac taaaaacgct tatgttatag
     7321 taccagaggt acttggaaac aaagtgaaaa aagatgattt ttataagtta gtacaaaact
     7381 caattaattc tgggaatact tctatttctc tggatgaaag taaagtttat gaagcaccaa
     7441 acaacacaaa ggattcagaa aagttgaaac aagctattaa gacattagat aagtatgttg
     7501 gtgtagtcgt tacatatgat tttagtgacc gtaaggaaac tttagatggt tctgaaatta
     7561 ataaatggtt aaaagtagac tcagaaaaga attatgacat aacttttgat aatgatgcta
     7621 tggtagaata tacaagaggt ttaagtaaaa aatacagtac ttttggtgac tcaagaccat
     7681 ttaaaactgc cacaggtaaa gatattacaa tttcaggtgg aatttatggt tggttgatag
     7741 ataaaaagaa agaagctgaa gcattagttg atgttgtaaa agctggtaaa aatgttacaa
     7801 gagaacctat ttacatacaa aaagctgtat ctcgtaataa agatgatatt ggaaatacat
     7861 atgtagagat tagtctttct ggccaacata tgtggttctt taaaaatgga gaaatgcttg
     7921 tatcaacaga cgttgtaaca ggtgatttag ctagaggatt tgctacacca tcaggtatat
     7981 atcctcttaa ttataaggct agaaatgttt cattaactgg tcaaggttac agttcacctg
     8041 taagttattg gcttccgttt aatgggaaca taggcattca tgatgctact tggagaaatt
     8101 cttttggtgg aagtatatat aagtcttcag gttcacatgg atgtgtaaat acaccttttt
     8161 ctaaggcaaa gaaaatatat gaaaatatag aaccaggtac accaattatt ttatactagt
     8221 aaatgtgttg ctaacagtta agcgaagtca tcttagttta tttaggatgg cttccttttt
     8281 atataaagct tattgaagtt tacagaaaga ctacttaaaa ggagaaatac ataatgaatg
     8341 aaaaattaac taaaatttta gaagaagcta agaatgatga tgaatttaga aaagaactta
     8401 taaaaacaag agaacaacgt gatgttatgg attcattttg taaggttgtt acatctagag
     8461 gttatgatat gacagtaggg gaactttttt ctatgggaga agaatataca tccaatctac
     8521 tgaaaagtgt aaatggagga gcagcatatc ctattgaaga ttggagtgat tggtacgagg
     8581 atttttttat aatgttgtcg aatatataga gtaactaatg atattatcta cttaagatta
     8641 atacattgcc ttaaaataaa actattagaa ctaggtgtaa gttttatcaa tctttaaaaa
     8701 tattgattta tttctaacta gatataaaaa aattagatta aaaataaggt gacaaaaatc
     8761 gatatagctt gatgatttat aaaagttaga taaaatagtg cgagatattt ctaaaaattc
     8821 aaaaaaaaca ataaacttca atggttagag gttgaaaaag aatataaaaa acttttatat
     8881 gtaataaatt aaattaaaat tataaaaaaa ataaaataaa aatttaaaat gtcgacataa
     8941 cgttaatttt aaaacaaaat aacgctattg acaaactata tcgctttaaa ttataattat
     9001 tgaaaataat ggtatttaaa aaccatactc atgtcatagt ctaaaagtaa gtcaaaaaag
     9061 gatgtaaatc taggcaagta ctattctaaa atatgtttta ttaagaaata aacgttgaaa
     9121 aatattaatt attatataaa aatgctgttt atactttaac aatgaggtag taatctaatt
     9181 agaataaaaa aatctaattg gttatataca tagtaaaaag gtaagagagg tgatttggat
     9241 ggcttttgaa ataataaaaa gcattgttga ggcagagcag acagcagaca gtatcaaagt
     9301 aaaagctgtt actgatgcag agtctatcag agctgatgct gtaaacaaat gtgaaagcat
     9361 atttgctgat gtaaaaaaac aagcaaagct tatggaagaa actcttattg agaaggcagt
     9421 caccgacagt agagcagagg ttgataaaat cttagctaat gctaaaagtg aatgtctgaa
     9481 aattgaaaaa actgctgaag aaagaaaaag taaggctatt gaagcggtta ttggaaaggt
     9541 agtgagataa tatggcaata gttaatatga acaaaatatc tattgttggc ttagaagctc
     9601 aaaaaagcca gattttaaag ctacttatga accgtgggtt tgttcagatt gatgatagtg
     9661 cctttcttac tgaggaggag gaattaaaaa attatcttgt aaaagatagt gaagaatctg
     9721 aagttataat gctagagcag aaaatgtctc atgtaaacca agcaattaat attgtttctt
     9781 cactagtgaa acaaaaaaaa tccctacttg caccaaaaag agagtttaac aaaattgata
     9841 gtgaaaaaga agggcaagtt tatgctgaag ctctggagtt aaacaatttg cacaaggaga
     9901 taggtacaat aaggtctaac ataaacttac ttaacaacaa taaaagtatg ttagaaccat
     9961 gggtgaactt tgatattcct cttgaaaata tggaaactaa gtatacaaaa actatacttg
    10021 gaactgtgcc cataagcgca aacattgatg ctttaagagt aaaattagaa gatgaaatac
    10081 cagaaagtgt tattgggatt gtcaattctg acaagttaat gaactatgtt tatttaatta
    10141 ctttaaaaga aacttttgat gatgttttag aagttttaaa agaatttaat ttttcagtgg
    10201 tttcattacc aaatgaagaa ggtacaccgt tacaagctat caagaaatac aatgaaatga
    10261 taattagaca ggaaaacgtt tctaaggaca aaacagagga aattaaaaga catgcagata
    10321 gtatacacat gcttgaaaac ctatatgatt tttacactat agaaagagac caaaaaaaga
    10381 ttatagagag acttgtcaaa acaaaaacaa cattctgtct taatggttgg ttaccaagca
    10441 aaagggcaga tgaattaatt aaagaaatta cagaaaagtt taattgttgt gttcagactg
    10501 aagaaggaag taaagaagaa ggcttcccaa ttctgcttga aaacaataaa ttagtaacac
    10561 cttttgaaag tgtaactaac atgtacagct atccaagtac aaaagatatt gacccaaata
    10621 caattttaac tttcttcttt gttgtattct ttggaatgat gctaagtgac gccgcatatg
    10681 ggttaataat tgcagtagtc tgtggatttg ttgtatataa attaaagata caaaaaggtg
    10741 aaggaaattt aattaaactt attggtatct gtggagtttc aactactgta tggggtctta
    10801 tattcggggg tattttgggg gatttaattc ctataaaagc attaattaat cctttagagg
    10861 atgttatgac acttatggga atgtcgttac tgttcggtat tatccatata tatgtaggtt
    10921 tgggaataaa agcatataca ttaataagaa gtggaaaggt aatagatgca atctttgaca
    10981 taggtttttg gtatttgtgt ataacaggtg tatgtctact tataatacct tttgttgcag
    11041 gggatatagg tgtattttca gaagtaggaa aatatttagc aattataggt gctataggtc
    11101 ttgtacttac tcaaggaaga agctttaaag gtattcctgt taagcttttt aaaggatttt
    11161 caagtctcta tggtataaca agttattttg ctgatatatt atcttataca agaattatgg
    11221 cgctttgttt aacaacagga gttatagcac aagttatcaa ccttttagga gctatagctg
    11281 gtccgatact tgcagttgta attggtgttg taggacacac aataaacctt cttataaatg
    11341 cactaggtgc atatgttcat acaagtaggt tacagtatgt agaatttttc aataagtttt
    11401 atgaaggcgg aggagttcct tttgttccgt ttaagtataa aacaaaatat acaagcatta
    11461 ataaaaagga gatgtaaata tggaattttt aaacgcgtta ggaaatggtc aatttttggc
    11521 aatatcagga gctgctatag cagcattatt tgcaggtatg ggttctgcta aaggtacttc
    11581 aatagcaggt caagccgcag caggtgttgt aacagaagac cctagtaaat tcggtcaact
    11641 tctagtttta caacttcttc ctggtacaca aggtctgtat ggatttatca tagctttcct
    11701 tattttatca aaagcaggaa ttattggtgg agatgcaata ccaacatcag ctcaaggttt
    11761 acaacttctt atggcaggac ttccaattgg tttagttggt ttagtatcag gtatagcaca
    11821 aggtaaagca gcagcagcag gagttggaat agttgcaaaa agacctgaag aacttggtaa
    11881 agctataaca tttgcagcaa tagttgaaac atatgctctt ttaggactac ttgtttcttt
    11941 cttagcttac aatggaattg caattggtta attataaatt ataattcata agtaaaaaaa
    12001 atgaaaagga ggaagcactc ctatgggtaa tgaacaaaaa atgattgaca gaattatagc
    12061 tgatgccaaa caagaagcac aagaaatatt ggataaagct aaaagtgaag cagatttaaa
    12121 agtaaattca gctaatgaaa aagctgaaaa ggaaatggct tcctatacaa agcttgcaga
    12181 ggcagaagca gaaaaagcgg cttctaagga aatatcagga gcttatatgg aggctaaaaa
    12241 gcaaatatta tcaaaaaaac aagagatttt agaagaggta attttagaag ctaaaaacaa
    12301 acttttaaat cttaaagata atgaatatga agagattata ctaaacatga ttgaaaaatc
    12361 aaattgtaca gatgattcag agatagtatt atctaaaaaa gataaaaaaa cattaaaaga
    12421 tgtgctatca aaaaaaggca ttaaagtgtc tgatgaaact agagatatta ctggaggttt
    12481 catagttaaa aaaggtgaca ttgagtacaa ttattcattt gaagccataa ttgcagtaga
    12541 gcatgaatat attgaacaaa ttgcagcaga aattttgttt aattaaggag gggttaaaaa
    12601 atggcagaag aaaaaactta tccctatgcc gttgcacgaa taagagtgct tgaaaaacag
    12661 cttcttaaca gacaaatgtt tattcaaatg gcagaagcca aaagccctga agactcttta
    12721 agaattatag ctgaggcagg ttatgctgat acatcaaata ataatataca taattttgag
    12781 aatattttga ctcaagaatt atcaaagaca tatgaaattc ttaaaagtct agctcctgaa
    12841 gaaaaatttg ttgatgtatt tctttataaa aatgactatc acaatttaaa agtgctgatt
    12901 aaagaagaaa tttctggtgt agatggtgag aaatatttaa ttgatggagg tacaattcct
    12961 cttattaagc ttagagaatc tttagcaaat cgtagtttta gtgatttacc taagataatg
    13021 tcatcagcta tattagaggc atttgatgtt tataataaga cacaaaatgg ccagatggtt
    13081 gatattgttc tagataaagc aacctttacc agtatgaaag aaactgctaa agaaagccaa
    13141 aacaaatttg taatagatta tgttatgaaa gtttgtgatt taacaaatct aaagagtttt
    13201 ataagagtca aaaatatgaa gaaagatttt gatatgttta tgaatgtgtt tgtttcaggt
    13261 ggctcacttg ataaggaaaa attcttagaa gctttttcct cagatactcc agcatcatgt
    13321 tttaaatcta cttcttactc tgatgtctgt aaaaatggca tggatagtgg ttttactgtc
    13381 tttgaaaaac tttgtgatga ctatcttatg gaatatgtta gaggagcaaa atttaagcct
    13441 cttacattag aaccactaat tgcatattta tatgctaaag aatcagagat aaaaacaatt
    13501 agaattattt tgactagtaa gcttaataat atagacgcag atactataaa agaaaggcta
    13561 agggatgctt atgtataagg taggagtagt tggagataaa gatagcatca tgggattttt
    13621 agctcttgga atagatatct tcccagccta tgatagtgac gaaattaaaa aaagtataca
    13681 taagttggtt gaagatgaat atgcgataat ttatattaca gaacaggcat cattgcttgc
    13741 aaaagaatca atagcaaaat acaaagatta ccaacttcct gccattatag ttattccagg
    13801 tattggagga agtatgggat taggtatgaa tgaagtaaga gaatctagta aacgtgctat
    13861 aggtgcagat atattattta aagaatagat gaaagttgaa taaaagacaa tctcaaattt
    13921 tattggtgct aaatatatgt gctaataaaa tataggaggg aatatttaaa ttttgataaa
    13981 gtatatgatt gcaggtgaga gaatatgaaa acaggcacaa ttgtaaaggt gtcaggaccg
    14041 cttgttgtag cggagggcat gagagatgcg aatatgtttg acgtcgtaag agtttcagat
    14101 aaacatctta taggcgagat tatagaaatg catggagata aagcttctat acaggtttat
    14161 gaggaaactt ccggtctagg accaggggaa gaagtagttt ctcttggaat gcctatgagt
    14221 gttgaacttg gaccaggtct tatttcaact atttatgatg gtattcaacg tccacttgaa
    14281 aaaatgtatg atatttcagg tactaatata actaaaggtg ttgaagtatc ttcacttgat
    14341 agaacaacta agtgggaatt taaacctaaa aaatctgttg gtgataaggt tgtagcagga
    14401 gatataattg gtggcgttca agaaacagca gtagtagaat gtaaaattat ggttccttat
    14461 ggagttaagg gtactataaa agaaatatat tctggtgaat ttactgttga agatacagtt
    14521 tgtgttatta cagatgaaaa aggtaatgat atacctgtaa ctatgatgca aaaatggcca
    14581 gttagaaaag aaagaccata tagagaaaag aaaacaccag attcacttct tgttacaggt
    14641 caaagagtta tagatacatt tttcccaatc acaaaaggtg gggtagcagc tataccggga
    14701 ccttttggaa gtggaaaaac tgttacacag catcagcttg ctaaatgggc agatgcagat
    14761 atagttgtat atataggctg tggcgagcgt ggaaatgaaa tgacagatgt tcttcttgaa
    14821 tttcctgaat taaaagaccc aagaacaggc gagtcactta tgcaaagaac tgtgcttata
    14881 gcaaatacat cagatatgcc ggttgctgca cgtgaagctt ctatatacac tggtattaca
    14941 atagctgaat attttagaga tatgggatat agtgttgcac ttatggcaga ctctacatca
    15001 agatgggctg aggctcttag agagatgagt ggtcgtttag aggagatgcc tggtgaagaa
    15061 ggttatcctg catacttagg ttcacgtctt gctcaattct atgagagagc aggaaaggta
    15121 aattgtctag gtatggatga aagagaagga acacttacag caattggtgc agtttctcct
    15181 cctggtggtg atacatcaga acctgtcagc caagcaactc ttcgtatagt aaaagtattc
    15241 tggggtcttg atgcatcttt agcatataaa cgtcatttcc cagcaattaa ctggttgact
    15301 agttattcac tctatcagga aaaagttgat gtatgggcag ataaaaatgt aaatgataat
    15361 ttctcagctc aaagagctca agctatgaaa cttttacaag ttgaatctga acttcaagaa
    15421 atagttcaat tggttggggt tgattcactt tcagatggag atagattgat actagaggtt
    15481 actcgttcta taagagaaga cttccttcat caaaactctt tccatgaagt tgatacttat
    15541 acatcacttc ataaacaata tagaatgatg ggacttatat taaaattcta taagtcagca
    15601 caagatgcta tcaagcaaaa tgttactata aacgatatta tcaagctttc agttcttgaa
    15661 aagattggtc gtgctaagta tgtagaagaa ggcgatatag atgcagctta cgattcaatt
    15721 gaagatgaaa tagataacga gcttgcagac cttattaaaa agaggggggc tgaagaacta
    15781 tgataaagga atataagtca atacaagagg tagccggtcc tcttatgtta ataaacggcg
    15841 tagaaggtgt aaaatttgat gaacttggag aaattgaact ttcaaatgga gaaattcgcc
    15901 gttgcaaagt tctagaggta aatggagata ctgcacttgt tcagcttttc gaaagctcaa
    15961 caggtataaa ccttgctgaa agtaaagttc gttttttagg aaagagtctt gactttggag
    16021 tttcaccaga tatattaggt cgtgttttta gtggaatggg tagacctatt gatggaggtc
    16081 cagaaattat accagataaa agacttgata taaatggagc tccaataaat ccagcagcac
    16141 gtgattatcc ggccgagttt atacaaactg gagtctctgc tatagatgga cttaatacat
    16201 tggttagagg gcaaaaactt cctatattct cagggtctgg tttgccacat gccaatcttg
    16261 cagttcagat tgcaagacag gcaaaagtac gtggaactga ttctaagttc gccgttgtat
    16321 ttgctgcagt tggtataact tttgaagatg ctgaattttt tataagcgat tttaaagcaa
    16381 ctggtgcaat cgaccgttct gtagtgtttg taaaccttgc aaatgaccca gctgtagagc
    16441 gtgtatctac acctagaatg gcacttacag cagcagaata ccttgctttc gagcaagata
    16501 tgcatgtact tgttataata acggatatta caaactatgc agaggcgttg cgtgaagttt
    16561 cagcagctaa aaaagaagtt ccaggacgtc gtggatatcc aggatatctt tatactgacc
    16621 ttgctactat gtatgaaaga gcaggaaaaa tgaaagggca tgaagggtct ataactatga
    16681 ttccaatact tacaatgcct gaggatgata agacacatcc aattcctgac cttacaggat
    16741 acattacaga agggcaaatt atacttagcc gtgaacttta taaaaaagat atacagcctc
    16801 ctatagatgt tttaccatca ctttctcgtc taaaagataa aggtattggt aaaggtaaga
    16861 ctcgtgaaga ccatgcagat acaatgaacc agctttttgc agcttattca agaggtaagg
    16921 atgcaaaaga acttatggct atacttggtg aatctgcact ttctgagatt gatttaatgt
    16981 atgctaagtt tgcagatgaa tttgagaaag aatatgtttc acaaggattt agaactgata
    17041 gaactattga gcagacatta gaaatcggat ggaagcttct tagtatgtta ccaaaaagtg
    17101 aacttaaacg tatccgtgat gaatattttg aaaaatatat gcctaaggag tgatatctgt
    17161 ggctagatta aatataaatc caacacgtat ggaaatgact agacttaaga aactccttaa
    17221 aactgctaca agaggtcata agcttcttaa agataaactt gatgaactta tgaaacaatt
    17281 tcttgagatt gtaagagaaa ataagcgatt gcgtgaagaa gcagaaaatg ctcttgatac
    17341 tgcttataaa aactttataa tcgcaagagc agttatgagt caggagtatc tgggttcagc
    17401 acttatgatg ccaaagcaat cagtttcagt agatgtttct acgagaaata taatgagtgt
    17461 tgatgttcct gtgtttgact ttaaaactga aaacaatcaa agtgatattt atccttatgg
    17521 tcttgctttt acatcaggtg aattagattc tgctatggaa gctttctcag atgctatgca
    17581 accactttta agacttgcag aaagtgaaaa aagtgcacag cttcttgcac aagagataga
    17641 aaagacacgt agacgtgtta atgctcttga aaatgtaatg ataccaaatt acattgagac
    17701 tattaagtat atagctatga agcttgaaga aaatgagaga gcaagtacta ctagacttat
    17761 gaaggttaag gatatggtgc ttaaaaaagc acttgaagaa aagaaaaaaa atgatttggt
    17821 tgtataatta aatacttata aaaataagtc taacattcta ctttatagag gttagactta
    17881 ttttttatgt ttaaattaaa atgtagatac tataaaaatt aatatattgt ttaaaaacag
    17941 actgtattta actaaaaata atatatttaa caaaaatttc taaaaattaa cgaactgtta
    18001 atttacactt aactttatat taacaaatta ggtgtattat ataaacataa gcaaaaacaa
    18061 taaaaaacaa ataaaaaaag gaaaaaacac aaagggggaa gatatgaaag aatttatatt
    18121 tacttgtctg tgcattgtag tgattacatt tatgacaagt tgcatatcaa caacaccaca
    18181 aactactcaa ggaaaaggaa ataataaact tacaatatat gcagcactag aggaagagca
    18241 gatagcttat tatatggaag gttttagaga agagtatccc aatatagatg tagagatagt
    18301 tatggattct catggtgtaa tagcttcaaa actattagca gaaaaagata atccacaagc
    18361 agatattata tggggattat cagcaatgaa catgatagag ctaaaaaaag atggtgcact
    18421 tgaagcatat aagcctaaaa attatgataa agttgataaa aaatttaaag atactgaaga
    18481 tgatgtatct tgggtaggaa tgagtgtccc agagacagca atagtagtta attataagga
    18541 acttaagaaa ctaggtatag atattccaaa aagttatgaa gatttaataa aaccagaata
    18601 taaaggactt ataacaatgc caaatccagc atcatctgga acggggtatc ttacagtatc
    18661 agggcttatt caaatgatgg gtgaaaaaga tgcttacaat tacatggata aattacataa
    18721 aaatatagcc acatatacac actcgggttc aaaacctgcg aagttagctg gctctggaga
    18781 gtatccaata ggtattacac ttggatatag agctacacaa caattatcta gtggagaacc
    18841 tatagaggtt gtatttccaa aggaaggttc aggctgggac ttagaagcaa atgccttagt
    18901 taaaaaagat aatataaaaa aagaagcaaa gctattttta gattgggcaa taagtgattc
    18961 agctatggat agatatgcac atgaagtagc agttacttcc ataaagacag ataatccagt
    19021 tccaaaaggt tattctaaac aaccatttga acagcttata aaagaagttg atttaaactc
    19081 tgctgccaaa aacagaagta cagtacttaa aacttgggaa tcaaaatacg gttcaaaatc
    19141 tgaaagtaag taaagtttaa attaataatt tagatgaaag aattttagaa gaaaaagtag
    19201 gatataaaat ttaaaataaa aaaataacta tataaggaga gataagatat gagttactta
    19261 aaaataaata atgtgtttaa aagttatgac caaaaaaggg ttttaaataa tattagtttg
    19321 gatattgaag aaggagaatt tctatgttta ctcggaccta gtggatgtgg taaaactaca
    19381 ttacttagaa taatagcagg tcttgaagat gtaaatagcg gaactataat tctccaggat
    19441 aaagatatta caaatttaga gccatcaaag agaggttttg gtatagtatt tcaatcatat
    19501 gcattatttc cgaatatgac tgcttataac aatatagctt ttccactaaa agagagaaaa
    19561 gtttcaaaag gaaaaataga taataaagtt aaggaagtac ttgaaacagt tggattgact
    19621 aatgaagctc ataaatatcc taaagcatta tcaggtgggc aacaacagag gattgcaata
    19681 gcaagagcat tagctcttga accaaagttt ttacttttag atgaaccaat gtccgcatta
    19741 gatgcaaagg ttagacacaa gcttagaatg gacattaaaa gattacaaaa agaacttaat
    19801 ataacgacaa taatggtaac acatgaccaa gaagaagcta taactatggc agataaaata
    19861 gctattctaa atggaggaga tataatgcaa atagggactc cagaagaaat ataccaaaat
    19921 cctcaaaacc tatttacagc tcaatttata ggagatacaa actgttttga taatggagat
    19981 tctatattaa ctgtaagacc agaatatgtt caaatagaga aatcgacaaa agaaaactac
    20041 caaggaataa tatcaaatat agagtttaga gggaatttat tgagagttga aataaaagat
    20101 aaattaaatg aaaatttcat aataagtgat gcttctataa aagagtgggt taatttaaat
    20161 ttagtagaag gtgaccttgt aaaaataagt atagacgaaa aatactattt aaagtatcca
    20221 aaagttaaag gagcataagt gtatgaataa taatgctata agttctaaaa gtataagtct
    20281 taaatcaaaa atgaatttag atataaaaaa atgtatgctt acagtattag ttatgtcttt
    20341 agtaatattt ttattagcac ctataatata tctatttgta caggcttttt tagataaaga
    20401 tggagtcttt gtaggattac aaaactttca agaatacttg gcttctgaat ctctattaaa
    20461 ttctctaaaa aatacagtat ttgtatcagt tatatcaact gtaatatcac taacacttgc
    20521 attagtatat gcatatgcat taactagaac aaagattaaa tttaaaggtt tttttaaatc
    20581 attagctatg tttcctatgt ttgctccaac aatgatgcat gcaataggtc tagtatattt
    20641 atttggaaat caaggtttgg taactaattt acttggagtg catgttccaa tatatggagc
    20701 agtgggaata atattggcac aaattatata tgtatttcct caatcatttc aaatacttta
    20761 tatatctcta agttcaaccg attatagact atatgaagct agtaatacta tggggataag
    20821 caagataaga gaatttataa gcataacaat accaagtata aaatactctt taataagttc
    20881 tgcattcgta tcgtttacat tatgttttac agattttgga gctccaaagg tgataggagg
    20941 agagtttaat gtattatcaa tagatgtata taagcaaatt atagggcaac agaatatgcc
    21001 aatgggagca actgtatcaa tattactttt aatacctgca ataatatcgt ttatagtaac
    21061 taaaaaaatt gaaaaaaatc aaaattcata tataacttca aagtcaattc catataaggt
    21121 taaaactaat aagttaagag atagtatttt aggaggggtt gtaggaatta ttgcatttgg
    21181 tataatagct gtaatgttaa ctgtaatctt atcttcatta atatcagttt ggccatatga
    21241 tttaagactg acactggaac atttttctat gaaaaacagt attgatggga ttaaaccatt
    21301 tataaattca ataaaaatgt cactattgac agctattcta gggacgagct ttgttttctt
    21361 atttgcatat ttgaatgaaa aaacagaaaa agctccatat ttaaaatcat ttgggtattt
    21421 tttaagtaca ttgcctatgg cattgccagg attagtttta ggtatagctt atgtattatt
    21481 ttttaataaa ttagagttta attttatgga tacaatatac attaagaatt tgtttaatgg
    21541 attatatgga acattactta taatggttat atgtaatata gttcatttct tttcagtagc
    21601 atttataaca gctactactg ctattaagaa agtagataaa gaattggata tggtagctaa
    21661 atcaatggga ataaacagcg catcaattct taagaacgta actataccac tttgtttgcc
    21721 agcagtttta gagaacttta tgtacttttt cctaaactcc atggtgacaa tatcagcatt
    21781 agtttttata tatactgcaa gttcaaaagt agctgcaata tcaattgtac aactagatga
    21841 taaggggagt actgcacagg cagcatcact agctgtgtta atattagtca caaatatatt
    21901 agtgaaagta atatatgaat ttataaagaa tcgtttagaa aaaagaacaa actttaatta
    21961 aataatttat cttataaaaa cacaaattta aaaaagtcta ttcttgtgat ttattattta
    22021 aagaatagac tttttaaatt tattattgat attgtttatt aattttcctt ttgttatcat
    22081 acatttgaga atctgcacga cgatatgtat caaataaaga aacatctact ttttcatcaa
    22141 aaatagcata tccaatagac attgataatt ttaattcatt agttgagttt tcttcaacta
    22201 tttttaaatt aatattattt ataatattat ttatttcttt gtgagataaa ttttttagaa
    22261 taattatgta ttcatcacct ccaatacgat aaactatata ttttttattt attacatatt
    22321 gtataatttt acttgcttgc ttaatatata agtctcctgt ctgatggcca aatttatcat
    22381 taatcttttt taaaccgtct aaatctattg atatgataga tattagattc tttagatttg
    22441 aattgtttaa gttattcata tctatttcaa aagcatttct attttttagg cctgttaaat
    22501 aatctgtatt agcaaaatca gtatatgtag gattagatat tcttttaaat agaatcactg
    22561 caatcaatgc tgatattaga caagttaata atgctattat tggtgtaact atacttataa
    22621 atttaaatgt attgtattga tgttttgctt caaattcaat tcctattact ccaacaacct
    22681 taccaacatc attgtgaata ggaaaatacg atataaatat gtatccccaa gatgttgatt
    22741 taatcttttt aggtaacaat atatgattat ctaatgcttt gttaaggtct ccccaaatct
    22801 ctttttctat caaatcacca gcatttctaa aatcactcct agatgaattt aaaccatcaa
    22861 caacatatac tagtgatcca tcttttgttt ttttagcagt atatagatac atgactccag
    22921 tagtattttt tacagattct aaaaggattt tagaactttt ataagacttt tttgattggt
    22981 cttctttaga attaatttca gtaaaggtat ttttatcaat atttctttct acatattcat
    23041 agatatgttt tgacctctca cttaaagagt atatcatgct cttataagtt agtgtataat
    23101 taaaaaaata tacacatgag gaagatatta ttacaataat tgcagtaagt attgaaacct
    23161 gcatatcaac tctattgaat tttttattta ttttcatagc ttaaatgtct tgtgaaaaga
    23221 acctcccaca atctccccat aattaatata tatctataaa tttcaccata atatattcat
    23281 atattatcaa atttgtgtgg aaattacaat tagtaaacat gcttaaagca ttcaaatata
    23341 gtttttttat tattatagta tactatttta tgtataaata aaattatata taaatcattg
    23401 atattggttg tttattaatg gaagataccg tataaaatat tctaattggt tacgatatgt
    23461 atacttaatt gaagttaagt taaaggttag aatatgtatt gatgaaatgg ctagatttat
    23521 attcataagt attaatttta tcatagatag gtaaaatact gtatcttaaa cataagtatg
    23581 attgagatgc aatcctgaga tatgataata taaattgaag gatagtgaac tcaatcaaat
    23641 aaaatgatta atctagctat ataagcaaat aaatttaata taaaagaggt ggaaaatgaa
    23701 agacagactt aatttaacag aaggaagaat tacagaaaaa ttacttaagc tatcacttcc
    23761 aataatgggg acttcattta tacagatggg gtataacatg atagatatga tatgggttgg
    23821 taaagccgga agtaaagcag tagctgcagt tggaacagct ggtttttttc catggcttgc
    23881 aatggcattt attatgattt caaaagtagg tggagagata aaagtagcac aaagtattgg
    23941 tgcaaataat ataagcacta ctaaatcata tatgaagtca gcaatagaaa taaatattat
    24001 actagcaata atatatacaa tttctttaat agtacttaat aaacaattga taggtttttt
    24061 tagattagga gacttagaag ttataaccat gtctagacag tatttgataa tagtagctct
    24121 aggtatggta ttttatttta ttaatccagt atttactgca attttcaatg gtcttggaaa
    24181 tagtaaaact ccttttcgta ttaatacagt aggattaata actaatatta tacttgaccc
    24241 tatattaatt tttggatggg gatttgctcc aaaacttggt gttgcaggag ctgctattgc
    24301 aactgtattt gctcagatag tagttactac atgttttata tatataatag ttaaaagtaa
    24361 agaagaatat tttaaattaa gactatttaa agaaatagag actaaatatt ataaagttct
    24421 gtataaattg ggattaccta tagcaataca aagtggtatg tttacagtat tctcaatgct
    24481 attaggagta atagtagcat catgggggcc tgtggctgtt gcagtacaaa aagtaggctc
    24541 acaaatagaa gcaatttcct ggatgacggc tgaagggcta tcagttgcat taggcagttt
    24601 tgttggacaa aattatggtg ccaagaagta ttctagaata aataaaggtt gcaaaatagc
    24661 tataactgta tcttctgtac ttggtataat aactacttta gtattagtgt ttgcaggaga
    24721 gagtatattt tctattttta tagatgaaag tgaggcaata gaaaaaggag cagtttatct
    24781 taaaatatta ggctattctc aattttttat gtgtttagaa ataataacaa caggagcttt
    24841 caaaggatta gggagaacct atataccttc tattataatt actatactga ctggtgcaag
    24901 agtaccattg gcttacttta tatctagacc agaaatgttg ggtctaaatg gtgtatggtg
    24961 gagtataacc ttatcaagta tattaaaagg tatattaatg ataagtctat ttatatcttt
    25021 attaaaactc ggaaagttgt acaagatttc tgaataaaat ttaaattatc ttaaatgtat
    25081 agacattttc tttgtttgta tataaaatta tcttattata taaaagattt aataaggagt
    25141 gaattaaatg aaagagccaa tttataaagt tatagaaaat catgttagag agttaataaa
    25201 ttcagacagt ttgaaagaag gggatttaat cccttctgaa aaacagctga gtgaagaatt
    25261 taatgtaact agaatgactg taaggtctgc acttaataat ctagttaaag aaggctatat
    25321 aactagacaa agaggtgtag gtagtatagt tattgcaaat aatatatatg ataatatatc
    25381 gtctgtaagt ggatttacta aagagatgga aagtaaaggc tataaagtat ctaatatatt
    25441 ggtatctctt gaaatagttc aggcagatga aaatttatca agtaagttga atataagttt
    25501 agaagaaaat gtttgggaaa taaaaagagt aagattagct aatgatgtaa gagtttcata
    25561 tatgattact tatatgccag ttaagttatt tccaaattta aataagactc attgtgaaaa
    25621 ttctctttat aattttgttg aagaagtgtg tggatacaag atagccatgt cagagagaga
    25681 agtacaagct gtaatatcga atgaagagtg tatggataat ctaaaattag aagaaccaga
    25741 gccactttta tatataagcc aaatttgtaa gttacaaaat agtgagattt ttgagtactc
    25801 acacacatat cactatggat atacattaac tttgaatgct gttgtcgaat aaaatttaaa
    25861 taaaatgaaa aagttttctt gaaatgtata tacatttggt atataataat atcaagaatt
    25921 aaatgtatat acatttaaaa ataagatata tacattaaga aaaaattaat agaaaaacat
    25981 attttaaatg tatagtttaa aaaacttatg acttaaaaaa tatttaatga atctaatttg
    26041 gaggggaaaa aatgataaaa ataatgttag cttgttcagc aggaatgtca actagtttat
    26101 tagttacaaa aatggaggat gcagcaaagg aaaacggtat agaagcaaaa atatgggcta
    26161 tatcagaagt gaacttaaaa aatgaaatag aaaattgtga tgtcttactt ttagggcctc
    26221 aagttagata tgtattagga aaagcaactg aaatggcaaa accatataat attccagtag
    26281 aagttataaa tatgatggat tatggaagat gtaatggaaa agctgtgtta gatagagctg
    26341 tagagttaaa tagttcaaaa taaagttgta ctggaggatg taaaatggaa aaattcatgt
    26401 catttatgga taaatatata gtgcctgtag ctgctaaaat aggagctcaa aggcacttag
    26461 tagcagtaag agatgcattt atagtaatga tacctataac tatggtaggt gcattgggaa
    26521 cattgataaa taacttacca ttggaagcat acaaaaattt gatggcaagt atatttggag
    26581 aaaactggac cacatttgga ggcgaccttt ggtggggagc aataggtaca atggcagtat
    26641 tcttagtaat aggtgttgct tattttctag caaaatctta tgagagtgat gggttacaat
    26701 ctgggcttat agctttatct atctttttta taatggctcc acaaattggt aaaatagttc
    26761 ctgagggagg aactacagta gttgaaggat ggggaatgat acaacaaacc tacttaggta
    26821 cagcagcttt attctcttct atattaatag gattattatc aactgagata tttgtaagat
    26881 tatctaaggt aaagaaatta actataaaaa tgccagatgg agttccacca gcagtttcaa
    26941 gatcatttgc aaagttaata ccaggaatgt taactataat gatatttact gtaataggaa
    27001 tatttataaa aatgctttct aatggaagtt tcttaacaga tattttaaat acttatttag
    27061 gagcaccact atcaaatgta gctgatagtc taggttctac aatgcttatt gcatttataa
    27121 ttcatatact ttggactgtt ggacttcatg gtgcaaatat agcacttcct tttacagaaa
    27181 ctatacttat gaaattaggt ggagaaaatg ctgccttggc tcaagcagga gcaacggaag
    27241 gataccatgt acttgcaggt gcattctttg atgcatttgt atatttaggt ggttctggaa
    27301 tggtattggg attaatagta gctttattaa tagctggaag aagacgtaaa gagatgatag
    27361 tattaggtgg tccacctgca atatttaaca taggagaacc attgatattt ggacttccaa
    27421 tagttttaaa tcctatattt atgattccat ttgtacttgc accagtaata tgtagtgctg
    27481 tatcatactt agcaatagat tttggattag tagcaccagt aattttacct aaaatacctt
    27541 gggtaacacc tcctatatta ggtggagcaa tggctacagg tgactggact ggtggagctt
    27601 tagcactatt taatttaata ttatcaatat taatatatat tccatttgtc atagcgtctg
    27661 aaaaaatgga agctaataaa ttgaaaatta acaactaggt agttttattt cggtaaataa
    27721 atgtatagag ttttaaataa atgtatgggg ttttgtagag tatattaaat tataggtaag
    27781 gatggtataa tgagatgaaa aagggactaa aaatagttac aattggagga ggttcaagtt
    27841 atactcctga gcttgtagag ggttttataa atagatatga agagttacct gtaaaggaat
    27901 tatggcttgt agatatagaa gctggaaaag aaaaattaga gatagttgga aatctagcta
    27961 agaggatggt aaaaaaagct ggagtagata tgaaaataaa tcttacttta gatagaagag
    28021 aagctctaag agatgcagac tttgtgacaa cacaacttag agtaggtctt ttagatgcga
    28081 gaataaaaga tgaaactatt cctttatctc atggagttat ggggcaggaa actaatggtg
    28141 ctggaggttt atttaaagcc cttcgcacaa taccagttat atttgatata ataaaagatg
    28201 tggaagaatt atgtccaaat gcatggatgg tgaattttac aaatccaaca ggaataataa
    28261 cagaggctgt attcaaatat actaatttta gaagatatat aggtctatgc aacgtgccaa
    28321 ttcatttaaa aaatgatgtt gccaaactat ttaatgtaga aagtgacaga ataagtatgg
    28381 attttgcagg tcttaatcat atggtttatg gtctaaatgt atctttagat ggagaagatg
    28441 ttacaaaaga agcaattgat aaatttgtca aagcagatat aagtatgcaa aatataaagg
    28501 caattgattt taatgctgaa tttgtaaaat ctttgggtgc aataccttgt ccatatcata
    28561 gatactacta taaaactaaa gaaatgcttg aggatgagtt gagagagttt aaagagggaa
    28621 aagctagagg tcaagtagtt aaagaattgg aagaacaatt atttgaatta tataaggatg
    28681 aaaaattaga tgtaaaacca cctcaacttg aaaaaagagg aggggcttac tatagtgatg
    28741 ctgcttgtaa tttaataagt tctatataca acgacaaaaa agatatccaa gtagttaata
    28801 cattaaataa gggttctata agagatttta aagatgaaca agcggtagaa gtaagtagtg
    28861 taataacaaa aaatggtcca aaaccaataa gtataggata tttaccagat tctgtgcatg
    28921 gattagtaag tcaaatcaaa tcatttgaag tattggcagc taaagcagct gtatatggcg
    28981 attatgaatc tgctcttttg gcactttgta taaacccatt gattccatct gatgacttag
    29041 caaaaactat attagacgaa atgttggaag ctcataaaga ttatcttcct agatttaata
    29101 gatagttgtc tatagaaagt aggttatggt atgactaaaa ttataataaa tgcagatgat
    29161 tttggatatt gtgaagctgt gaactatgga ataatttcag ctcataataa tggtatagtt
    29221 aggtctactt ctatgatggc aaatatgcct ggggtagaac atggagtagg cttacttaaa
    29281 gaaaataaaa ctttaaattg tggagttcat atgactttaa gttgtggtag accattatta
    29341 tctaacttaa aaactattgt tgataaagac ggttttttta ttaggaggat tacagacgaa
    29401 attattgaaa aaatggattg tgatgagatt tatagagaac tttgtgctca aatagatagg
    29461 gtaaaagggc ttggaataga tatatctcat ttagatagtc accatcatat ccatacttta
    29521 ttaagtttaa agccagtaat agagaagatt gtaaccaagt ataatcttcc aataagaggt
    29581 ggatttgaat ataatttaga gtattctaaa gtagttcctt taatagatag tttttataag
    29641 gaaaatgtaa gtgaagaata ttttataaaa aatatagacg aaataatgaa gtatgatgta
    29701 gtggatatta tgtctcaccc agctttttta gatgattata ttttaaattc tacatcttat
    29761 gctattgata gaacgaaaga gcacaagata cttacatcta aaaaagtcaa agaattttta
    29821 gaaaagaatg gattagtcat atcaagctat agagatattt aggaggtcga ataaatggat
    29881 gagaagatga tagagatatc ttttggaata attggatttg caggtgatgc gaaagggcta
    29941 gcatttgaag caataaaaga agcaaagagc gggaatatag ataaagctag agaatgtatt
    30001 gaaaagtcga aagaatcaat tcataaagct cataaattcc aaacagaatt gatacaaaat
    30061 gaagctaatg gtaataaaac tgaaataagt gttatactta ttcatgcaca agaccattta
    30121 atgacagcta tgaattttca acaattagca gaagaattta tagatgtata tgaaagatta
    30181 gaaagtaagt aatttaaaat tattattaaa aaagagtgta taaaaataat ttttataatt
    30241 attaaatgca ctcttttttt atttaagtag agtttagcaa tatagtatta tgttattgat
    30301 actttaaaat ttagacttct cataattgta aggtatacta gtaccaacaa ctacatcttc
    30361 aattttttca acaataagcc aatcatccat atttccttga tggtcaaaat caagaggtgt
    30421 tatttcttca acattttcaa attcaactaa gcataaacat tttttatgcc aacgaacttt
    30481 ttgcttttca gataaattta attttggttg attttcattt aaaattttag aaatttcatc
    30541 ttctgttaat ttaacatagt tttctacctt tttaacagtg gcttttgcag atatttttgc
    30601 agttcctttt tccataaaat aaagtaactc atcttcaaat actctactat gtggtatttt
    30661 tctaccagca gcacctctaa taaccattgt ttttgttcca gccaagattt tatctaagac
    30721 cttttctcct ttttttcctg cattatcaca atatactaaa tgtaccatgt tttgaatcct
    30781 ccttactaaa ttcattatct ataaattgca ttttatcatt gtttaactga aaagtcattg
    30841 tatttgtgtc caattcttta aaaaaatact gtttaatata agaaaacctt atctaagcta
    30901 tatataatta agattaatta ggaattttta tccttttaca aaataatatt gactaaaaat
    30961 agaaaaatgc tataataaat atgtgcttat gataatcaat atcatgtaag tataataaac
    31021 aagtaggaga agtaatcatg aaaaaaataa aatcacttgc aatatttata tctattataa
    31081 ctacgttagt acttgttaca gcatgttcag ataagaatac agaggataaa gataaatcag
    31141 agacaagagt tgtacaatct gttaaaggag aggttaagat accatcaaat cctaaaaaaa
    31201 tagttgatat atcaggaagt agtgaagaac tattattagc aggttataag cctgttgcaa
    31261 cagcaaatgt agactcatat gaaactgata aattaccaag ttacataaga gaagagctta
    31321 aaggtgtaaa gatagttggg cactcaatga tggatactat ggatatggaa gctatattag
    31381 aagtaaatcc tgatttaata ataatgagtc aaagacaaga aaaaatatat gaccaattaa
    31441 aagaaatagc tcctgtagtt atgatgaaag attatgcaaa tgactggaga agcaagttaa
    31501 ctgatgtttc aaagttattt gacaaagaag aagaagctaa aagttggcta cagaagtatg
    31561 atgaaaaagc tacaaagtta ggtaaggaag ttatagaaaa gaatggagaa aaaacttatt
    31621 taccagtgtt ggcaagttca ggtcaattta tggtatttag tgatggtgga ataggtactc
    31681 taatcaatga tgatatgaaa ttagctaaac ctaagaatat gccaaaacag gatggaataa
    31741 cactaccaat ggtaagtatg gaaggtttaa cagatataga tgcagaccat atagttgtaa
    31801 ttgctacaga agcagataaa aaagatttag aaaatagtgc tatctgggct caaataagag
    31861 cagtaaaaga tggtaatgta acaatacttg atgctgctcc atttttcagt caatcataca
    31921 atccaattgg aaaagagtta ttattagaat cagttaaaaa tgaattaaca aagtaattct
    31981 taacttagta ggtgtgagat aatgatattc atttttcaca cctattttta ataaatctta
    32041 aaattaaaat acttaataga tgatattata ctgacgatta aaagggaaaa ggaggtagga
    32101 taattgacta agctgagtaa aaaaaatata acttatttgt tgatattagt aagtatagtt
    32161 gtacttgtat ttggaattgt attatccata actattgggg caaaagatat gaatctatca
    32221 actgttatag atagtttgat aaaaatggaa gatggaataa atatgagaat agtaaaggat
    32281 gttagacttc caagggcaat agcagctgca cttgttggag gatttttagc agtttctggg
    32341 gcaataatgc aaggaataac gagaaatcct atagctgaac catctgttat aggaattaca
    32401 caaggagcta cattcgcaat ttcgatatca ctggtacttc aaaaaaaact tcctcaactt
    32461 atacatggaa gttttagtgt aatgatgttt gcatttattg gagcaagtat aagtggatta
    32521 tttatatatt ttataagttc taaatcaaga ggaagagtta ataatgtaaa attagctctt
    32581 gcaggagtcg ctcttggtac attgctaatt tctttggcat cagcaatatc tatgtatttt
    32641 aacctatctc aacaattaag tttctggata tcaggagggc ttgtaggagt gaagtgggaa
    32701 ggtataaaat tgctatttgt agctggtggt attggattta ttttagctat tattatggct
    32761 ccaagaataa ctatattaag tcttggagaa gaagtggcaa taggattagg acaaaaaact
    32821 aactttgtta gatttatatg tatagtactt gtaatattaa tgactggagc atctgtgtca
    32881 gtgtctggta atattatttt tataggtctt atagttcctc aaatagctaa aggaatagta
    32941 ggttcagatt ataagtatat aataccgagc tctttagtat taggagctgt attgcttgta
    33001 tatacagata tattaagtag gatgataaat cctccatatg agacaccagt aggttctata
    33061 acagcactta taggagtgcc aatatttata taccttgtta gaaaggggga gaaataggta
    33121 ctatgaaaaa tattataaaa ataaattcta aaacattact tgttatatca attttaatgg
    33181 tgcttatttt tatcactttt ttattgagtt taaatatggg ctcattttct atagaaccaa
    33241 tggatgttat aaagactttt ttagggcagg gtcagcgtaa tcatgaaatt gcaatattta
    33301 aattaagatt accaagaata gtaatagcac ttttggttgg ttctgcatta tcaacagcag
    33361 gtgtaatact tcaaggagtt acaaagaatg atttagctga ttctggaatt ttgggaataa
    33421 attcaggtgc tgcattgttt gtagtagttt atatatacct tatgaatggt aatgtatatg
    33481 aaggtatgag taacttgact gtatttacaa tgcctattgt tgcacttata ggagctttat
    33541 ttggagcatt tcttatatat tctcttgcat ggaaaggtgg tataaattca tcaagattac
    33601 tacttattgg tataggtgta aacatagctt ttacttcaat tttgacaata ttccaattga
    33661 aatttacaac acaggaattt aatagggtta tggcatggac atcaggaagc atttggggaa
    33721 ccagttggaa atatgtaata gctgtgcttc catttatagt gatttttatg ggtataacta
    33781 tttataaatc taaatatata gatgttttaa atttgggtga tgaagtatct ataagtcttg
    33841 gtgtagatgt agaaaaacaa agaagaagac ttataatata tgcagtaatt ttatctggtg
    33901 tatcgacagc agtagcaggt agtatttcat ttttaggttt aatagctcca catataggta
    33961 gaaaaataat aggaccaaag cataaaagat taataccagt atcagcattg ataggaacat
    34021 tattactttt aatagcagat actatatcaa gaaatcttct agcacctata gaaataccag
    34081 taggtatagt agtttctata ataggagtgc cgtactttat ttatttaatg ttggtaaatg
    34141 attaggaaag ggtgttgtta tgaactgtat aaatactaag aatctaaatg tatcatatgg
    34201 aaacacagat attataaaaa atttaaattt aaatatacca aaaggaaaaa taactaccat
    34261 cataggggct aatggttgtg gaaaatcgac aatacttaaa actataggta gaattattag
    34321 ccctaatagt gggaaaatat atataaatgg agaggacatc tacaaaaaga agtctaaaga
    34381 aatagcaaaa gatatggctg tacttccaca aagccctcaa gctcctagtg gtcttaccgt
    34441 ttcagaatta atttcttatg gaagatttcc tcataaaagt ggatttggta attctagtaa
    34501 agaagacaga gagattgtaa attggagttt agaagttact ggaattgcag aatttaagga
    34561 tagaaatatg gaagatttat caggaggaca aagacaaagg gcatggatag ctatggcttt
    34621 ggcacaagaa actgatatat tattactgga tgagcctaca acatatttag atttggcaca
    34681 tcaattggag atactaaaat tattagatga actaaataaa aaacaaggaa gaactattgt
    34741 catggtaata catgaactta ataatgcagc aagatttgct gaccacatga taggtattaa
    34801 gaaaggtaaa atagtttgtg aaggtgatgc atatgatgtt atgacgaagg ataatctaaa
    34861 agaattattt aatattgatg cagaaatagc aacagaccca agaacaaata aaccaatttg
    34921 tataacttat gacatggtgt aaatggtgac aacatggaga atcaagaagc attaagacat
    34981 gaaaaaatat ggattttatt atttaggtat tcgatacctg caataatagc aatgatggta
    35041 acttcacttt ataatgtagt agatagagct tttattggtt ctatggaagg tattggctct
    35101 atagctattg caggtcttgg tgtaactatg ccagtattta ctctaataat agcttttgga
    35161 atgttggtat cagtaggtgc tagcacaaga ttgtctataa agttagggga aagaaataga
    35221 gaagaagcag agaagatatt aggtaatgct ttgacactat ctataattat ctctttaatt
    35281 ataactatat taggattagt tttcttagaa gatatacttt ttattttggg tgcaagtaaa
    35341 gattcaatat tttatgcaaa agactatatg agtgtcatat tggttggaag tatatttaat
    35401 ttagttgcat tttctcttaa taatgcaatt agggcagaag gtaatccaaa attagcagca
    35461 aggacaatga ttgtaggatg tgtattaaac ctgatattgg accctatatt tatatttgta
    35521 tttaatttag gtataaaagg agcagctata gcaacagtac tctgtcaaat agtagtattc
    35581 ttttgggtaa tacattattt tataagaggg aagtccaatt taaaactaaa aaaatgtaac
    35641 ttaaaattga ataaagatat agtaaagaag gtatttgcaa taggaatgac accttttgct
    35701 atggaagtag ctataagtat aactcacatg tttacaaata attctctaaa ggtttatggt
    35761 ggagatttag ctattggagc tatgacagca cttacatcta ttttattgat gtttatgatg
    35821 cctgtgtttg gattgaatca aggtatgcaa actataatat catacaatta tggtgcaaaa
    35881 caatatgagc gtgcaaagaa aactttaata ttatctatca tagtgtcagt tgtcatattg
    35941 tcatttggat ttttagtagt acaagttttc ccagaagtat ttgtaggtat atttaataaa
    36001 gattcagatt tgatggatat agcagtaaga ggtataagcc taaatctaat tacattgcct
    36061 ataatgggga tttctattgt aggacctgtg tattttcagt gtataagtaa agttaaacat
    36121 tcaatgtttt taactctttt aagacaattt attttattta taccattaat cattgtactt
    36181 ccaattcaat ttaatttaga tggtgtttgg ttggcacagc cagttgcaga ttttattgct
    36241 atgataattg tattattatt cttaaaaagg gaatttaaaa caagccgagt gtaagaaatt
    36301 tagaaaataa aattaaattg gaaaataaaa agacttgtct ttataaaatg attaaaatta
    36361 ttttatagag acaagttttt tattttattt aaacggaact atccatactg gtttcttcta
    36421 gtttttttcc aaaagtcatt ttctcaaata tttcttgagg tagaggtttt gaaaaaacat
    36481 atccttgtat catatcacaa ttaatttttt taagataatc catttgcaag attgtttcaa
    36541 caccttctga tatactattc atatttaatt tttttgccaa attaactata gattctatta
    36601 catgattttc agcaatatta tctgcattag gtgagctaaa aaaagcacgg tcaagtttta
    36661 atgtgtcaac ttttatttct tttaacatat tgagtgaaga gtatccactt ccaaagtcat
    36721 ctagtgaaca ttgaaaacct tcttcatgga tttggtctat tatattaatt aatttttgga
    36781 gattttcaaa tacaagtgtt tcagttaatt caatttctaa gagcttagca ggaatgttgt
    36841 atttattacg aagcattctg tatttttcta aaaaatctat attatttaaa ttagcacgtg
    36901 aaaaattaac tgaaacaggt attggagttt ctccattatc tatccatttt cttatcatac
    36961 tgcatacttt ttcaaaaaca tagtggtcga gttttgtaat aaaaccattt ttttcaaaga
    37021 aaggaataaa ctcgtctggt ggtataagac ctttatttgg gtcttgccat cttactaatg
    37081 cttctgcacc tacaatagta ttatctttga ggcttatttt aggctgaaga tatactataa
    37141 attcgttatt atttaatgct tcctccattc tattttccat ttctttttct ttaatcattt
    37201 ttaaacgctc taaatcacta taaaaaacac atgtataaag tggatgattt ccaatgtttc
    37261 ctttattatt ttttcgtgca acatttgcac ggtcttgaat tgaaactaaa ggtagatttg
    37321 ggtcatcaat tttatataca cctatagata gagctataaa atatttacat tcttcatttt
    37381 tattgaaact attaatatca ttggtgatat tttctatgta cttaagaatt tctttttgag
    37441 gaatagtttt aataagtaca ttaaactggt cattagaaac tcgacaaagc aattctcctg
    37501 gttctagata attactaatg acatcatgta tgtactttaa tgttttatct cctgcttcac
    37561 taccaaaaga atcattaata agtttgaatt tctgaatatt tatagatacc aatgaatagc
    37621 tttctgatga agatgactgt ataatttttt tagcttcaat atcaaaacgt gctttactgt
    37681 aacctttagt tacagagtct acataggcga tttttataag ttgtgccctg ttcttttgat
    37741 ttataaacat aatcaatata ataagcaata aaaatagtat aacaattaaa atattgatga
    37801 agattgcttt ttttagaagt aaatcaaact gtgcacttgc tgtttttgtt ggaacaattg
    37861 aaaggaggta taagttttct tcttgtaatg gaagataatg cattgcctta gaaataccat
    37921 catttaatgt ataatacata gaaccagttt tgttcttttt taagtttatt ttcatttctt
    37981 caagtttatt tttatcaacc tgttttcctt ggtgtttaat catgtcaaaa aaatttacca
    38041 cattttttgg agcatttttg tttgctgaac ttacaataaa atttccatct tggtcgataa
    38101 tttgggaaaa tccttcacca tgaaagcttt ctacacttaa taaatttctc atagtatcaa
    38161 tagtattcca agcagcaaca gctccaacaa ttttatcacc attgtatagt ggtacagaat
    38221 ataaaattcc atatgtacca gattgtgatt taacaggagt aattgtttct ttgccttgaa
    38281 gggcttttct ataaaagtct tgtcctttaa cattacctgt atctttaagg gcacttacaa
    38341 aatttccatt agaatctata atacctaacc aatcaaattc aaataatttt aatttttttg
    38401 ataagtatct attgacttct ttttcactat cattaatctc aaaataagta tctcccatag
    38461 aatatagtgt cctaaatata cttttacatt tcacattaat tgtagtggaa atactttgtg
    38521 aaacttctac aagatatcgt tctgtttcat tatgtgttac tttatttaga ttcataataa
    38581 aagtgacaca tgttaatgac acgattaaaa ggagtgtcaa tagtaaagta gctattattt
    38641 ttttattttt tgtgttttca aagttcattc ttttgtttca tcctccatcc tttatttgtt
    38701 tacaaataag aattctctca gacatattat accaaatatc ttttcataca aagatattaa
    38761 attttattta tcccacttta catttagctt tatttatgtc ttcttagaga ttaatcaaga
    38821 tacgtttatt gtttaaatct tattttataa tattttgagt aaaagaggtt aaaaaaggaa
    38881 ctgttttcaa aataattttg atacagttcc ttttcagtat agaaaatttt tatagtatta
    38941 gaaagaactt ttagcttatg tatctacata agtattatat ttataaaaga tattttgtta
    39001 caatctaaaa tgtagatgaa attctgtaaa tgccaaattc tctatgacca agactttcag
    39061 ctttggtatg tttaccatta cagacttcta ttatttcatt aaatatttct tgacctatat
    39121 ctgctatttt agcaccatct gttataattc tacctgcatt tatatcgata ttgtctggca
    39181 ttttgttgta tgtatcacta ttcccagtga ttttaataac tggtgaaata gggcttcctg
    39241 ttggagtacc tctaccagtt gaaaagatta caatttgtgc accacctgct accataccag
    39301 tgatagaatc tatatcttga cctggagtat ccataaaata taatcctttt ttatcaggag
    39361 gtacaatatc agcatattca agagcacctt tgaatggctt attgcctgcc ttatacatac
    39421 acccaagtga cttttcttca attgttgaaa gtccaccagc tttattgcct ggagtaggtt
    39481 gaccactacg taaatcttct cccatagcaa ttgctctttt ttctacatca cttacaaatt
    39541 taagaaactt atctttcatt tcttcatctt caaaacgggt agccaacaaa tgttctgcac
    39601 caattacttc tgtagtttca cttagtatgc tacttccacc ttcatctaca agaaggtttg
    39661 atgcaacgcc tattgaaggg tttgaagcta ttccacttgt tgggtcagaa ccaccacatt
    39721 caagtgctaa tgtaatttca ctcataccaa attcaactct tacttctttt gatacttcac
    39781 gagccatttt acttactatt tcagctccct tagaaactgt ttttaatgtt ccaccacatt
    39841 cttgtataac aacgtaattt accttttttc ctgttgtagc tatttcagat gctaattctt
    39901 ttgcttgaat accatcacat ccaagaccaa ctactaaaac tgcggcaaca ttaggatttt
    39961 ttccaaatcc tattaatgtc tttgctgtaa gctctatatc agaaccaatt tgacaacatc
    40021 catgttgatg agggattgct attgctccag gcacaagtga tgctattctt gtagctgtct
    40081 cacttgaaca tacagatgtt ggaataatta aaacatgatt acgaatacca aactgaccat
    40141 tttctcttaa ataacccatt aatttcattt taacttcctc ctatttttta tctccacgac
    40201 cacgtgtact ttcaatgtta tgtacatgaa catagtcacc agtttttatg tcaactgttg
    40261 caattcctat aatttcaccg tatttgtata caacatcacc tttttttaca tcttctgtag
    40321 ccatcttatg aaagacaggt atattatcat ttactatgta agactttcca tcaattgaaa
    40381 ctttttcacc tttaattaaa ggtctgacac atgtaactag attatctttt tcatttgcat
    40441 gaataattgc attcattaat tttacctcct taagtattaa tttacttcaa tatttttatt
    40501 tgttttaaaa atagattttt tctttttttc tcttttatct agataggaaa ctaacattgg
    40561 acaacatatt gctgtaacaa taatagcagc agcaacttgt actgttgaaa cagctgctat
    40621 tgtagctaga gtagggtcta tagctgctaa agcttcagga gtaccagctg catttccggc
    40681 tgttgtacct gtagctgcac ctactgctgg atgttctgca cgtataagtt tcattacaaa
    40741 gtacccaccc atacctgtta ttatagtaac tcctaaacca agtacaactc caggtagtcc
    40801 agcagttata atttgattaa agtttaatgc tgctcctaaa ggaaaagcga agaaaggaat
    40861 taataatgtt gtaccaggtg caagaaattc tcgtatatct tcatcaaggt taccaagtat
    40921 aaatccaagt agtattggta taagtgctgc aaatagtgct actataggta catttgcaag
    40981 tccagttact ccaaaagcaa gcattgtaaa aaaaggacca tcatttagag ataagataga
    41041 tattgctcca acatctgtgg agtctccata ttcacctgca agagcagcaa atagacctcc
    41101 atttgaatta gtcatggtag caactatggc aagaggtgta ataccaatta tacctttagg
    41161 tccaataaat ttactaacaa taatacctaa tactgcacca attataaatt ttgttgctgt
    41221 aagtactata cctttagtca atggtttacc agcttgtctg atattgattt gagctccatt
    41281 gcatagacaa aatagagcaa gtatactcat tgcaccagtt ttaaataagg atgttgtgaa
    41341 tcctccaatt tcaagagctt gtggacatac tgtattaaat agtgctccca ataacaaggg
    41401 acagaccata agtcctcctg gtatcttttt cactgatttt aagatgtgca tctagtatca
    41461 ctccctttca tttttatata ggtattataa aagcaattac tatgccaata tatagagttt
    41521 gtatctttag ttatcatgat tatttttaat aatcgattga aatgctgtag attacttata
    41581 attaaaagta tgattgacga gaatttcact tacaaagatt ctttttagca tataattgta
    41641 ttttttattg aaaacaaata aacgtcttat gtattaaaat taaacataaa acgttttgat
    41701 atatatagat atgtattatt tttaaacatc atcttctatt tcttttaact tacgccatag
    41761 agtggagcga gttatcttta gaatttcaca ggtttttgta gtatttctat tattttcgtt
    41821 ataaatttta tagatatatt ttttttctaa agtttttaaa tctaaattac tgcattcaaa
    41881 atcactatta tctttatcaa ctatattatt tgtatattca ttgctaagct tttctaataa
    41941 aatcgagtca aatgattcca caataacagc cctttccatc ataccttcca attcccttat
    42001 attaccttca tattctcgtt caagaagtgc ctttttaata tttggagtga taattaaagg
    42061 cgttttattg tatttgcttg aatatttatg tataaaaaat tctgataatt ttataatatc
    42121 ttcttttctt tcttttaatg aaggaagata taatgttagt atatttattc taaaataaag
    42181 gtcttgtcta aataaaccat tgcgcatcat attttttaag tctttatttg aagcacagat
    42241 aatttttaca tctataggaa taactttatc accaccaatt ctcataactt gtttttcctg
    42301 caataccctc aataatcgac cttgaagttc aagagggatt tcacttattt catctaggaa
    42361 gattgtgcct tcatgtgcta gttcgaatac acctgcttta cctttttttc tagcacctgt
    42421 aaatgaacct tcctcataac caaaaaactc actctctatt agagatggag gtatggcagc
    42481 acaatttaca gcaacaaaag gtgcattttt acgcttacta tagttatgta tactttgaca
    42541 aaatagttct ttaccaactc cagattctcc agtaataatc ataggagtat cataacaagc
    42601 atattttttt gcaagttcta tacaatcttt tgtcttttca ctagcatgaa ttatatcatc
    42661 aaaagtgtat ttagcagata gacctttgct taataagctc cttcttattg tatgttcaaa
    42721 agaacttatc tccttgctat cttgaaatgt tgaaacagcc ccttgaatct gaccatcaac
    42781 aattattgga actctatttg taataatttt tgtactccct atattttgta tttcaccaat
    42841 ttgaggttcc atagtctcaa ctacttgtgg aagaactgtg tttgggataa catctacaac
    42901 attatgataa agtgcatttt ctttttttat accaaatatt ttttcagcac tcttattaaa
    42961 taatgttatt ttaaaatttt tatcaactgt tattattcca tcatgtacaa aatctgtcat
    43021 agcttctatc tgtaatcttc ttttttgttc atccttggtt gcttcgagta tttgttctgc
    43081 ttgttgaatg gctctaagta tgttttcttc tctagattcg attaatattc ccttacaatt
    43141 aagtctctta acaatatgtg caacagtggt atctcctata tatgtgttta tacctttctt
    43201 tctgtacttt tctatagcat cataaatttc ttcttctttt tcaatttcaa tcatagttat
    43261 tttcttattc aatatttctt ctataatatc gaaaccgtag atgacattat taaatccaat
    43321 tattccaaat ggttcgttgg aattttttac ctccttgtag ctttttataa tatcataagc
    43381 atctacttta atttcaacta taggtatatt gtaagtagct tttaacatat tgtaagttcc
    43441 acctcggctt atgataattc tagtaccatg attaatactt ttttcagcaa gaggtatacc
    43501 ttcttttaga ttgccaaata caacatcaat atttgtatat ttgttttttt taactatgtg
    43561 aattgatttt tcataggttc ctttagtggg agcaataacg agtatttcct tcataataat
    43621 tcctccatat ttcatcaaat aaacttaaat attatcttat gactaaattt tattagttat
    43681 attttaacat tttactttat atttaattat aaataaagta tttttaaaag gaacattata
    43741 aaagactgta tcaaagttaa cttttagaat tataaagttt catcttttta cagtctttta
    43801 atatataata tctatttatc aaatctatct actttaaata atttcatatt tttattttgt
    43861 ttgccaagat ataattcact taacattata cctcctaata ttgcaaagag tataccattt
    43921 gctccataac ctaagcaata ccatagattg ttatttttat tatctggacc aataaaacct
    43981 aaattatcta aagttgaagc aaaacaacca caatatttat attcaacttc aatgtctttt
    44041 atatccttaa acatactttt caatctttgt tctaaaatat catacttttc attggctaaa
    44101 gtttcatttt ctatatcatc aaaacttaca tcttcaccac ctataattaa tcgattgtct
    44161 ttagtagttc ttagataatt atatgggtca cagttatctc taattaaaat gttgtttttc
    44221 caagaagcta tgtttttaag tggtttcgtt gctatattaa aagtatttga cttagttgca
    44281 aaatttcttt ttgtaaataa acttgtattg taccctgtag ctacaattaa ttttttacca
    44341 tgaactttgt atccatacga tacttcagca gtaaccttat catttgataa atctactttt
    44401 tttacttctg tattttcata tacttgaaga ccatttttta aacttacatc aatcaaatgg
    44461 tgactatatt tatatgggtc taattctcgt cctccattct ttgctatcaa tcctgatttt
    44521 aaatcaaagc taaaagggtt tgtggattca tcaatatatt caacatcaaa tccattttct
    44581 tttcttaagt tatattcttc tttgatagcc ttaacttcaa gtttgttggc agtataaagt
    44641 agagtatctc ttttggcata atcacactta tttccatata gctctataaa tgtatctaat
    44701 tcttcaagtg ctgaagtaca aaggttgtat gcttttagag catcttttag agtcataacc
    44761 tctgttaaat ctatcaaatt gtcatctaat tcatactgta atagagaagt tgtcacgcta
    44821 gtgcttccat gtgcaatcct tcctttttca agaatgacag actttatatt gtttttagct
    44881 agatagtaag cacaaataca tccagttacg ccaccaccaa caattataac atcagtatca
    44941 atatcatcag taaggtatgg aaattgtctt ggaactttat taatattagt aaaaatagga
    45001 tttcctttta catattcttg attcatatac ttcactccta aaatttatta tgtatgagtg
    45061 tattattttc tatagcaaat aatattatac attttaatta tctagttata tgggtatgta
    45121 tagataaaat gtgataacat tttagtataa aacatatgta taatatattc ataattacct
    45181 tattttaagt aatatagtat ataatgtttg ttaacaagta aaaagattga ataaaagtta
    45241 attaaaaatt taaagtagga ggtaaattta tgctttcaaa aaatatagct tctaaggtat
    45301 tagaaaaatg tctaattact ggaggagatt ttgctgagat ttttgaagaa gacactataa
    45361 ataattctat tagtttaata gataataaag tagaaaatgc aataggtgga agaaattatg
    45421 gtataggaat aagaatattt aaaggattga aaagtatata tgcttataca agtgacaatt
    45481 cattaaatag tctgttggat gtagcataca aagctgctgt tgctttaggt aaattggaag
    45541 atggaaaaag tgtaatatta aatgacagta ttaaaataaa taacatacat aatataaaaa
    45601 tttatccaaa ttcaatagga aataaagata aagtaagtgt tatgaaggtt gcatataaga
    45661 gtgcaaaaga atttagtaat gatatatctc aagtaagtgt cagttatttg gataaagacc
    45721 aaaaaatatt aattgcaaac actgaaggta tatatgttga ggatagaaga ataagaacta
    45781 gactagggat aagttcaatt gcatctaagg gtaatgaaaa tcaaactgga tttgaaggtc
    45841 ctggaggttg taaagggttt gaaatatttg aagaaataga cccagaatat tatgcaaaag
    45901 aatcagctag agtagcgcat actatgttgc atgctaaaaa ttgtcctgca ggtaatatga
    45961 tggtagcaat agataatgga tttggtggag taatatttca tgaagcttgt ggacattctt
    46021 tggaagctac atcagttgca aaggggaact ctgtatttgc agataaatta ggtcaacaga
    46081 ttgcttccac aaagattaca gctatagatg atggtacaat accaaactac tggggttcta
    46141 tgaatataga tgatgaggga aatccaactc aaaagaatat actgattgaa agtggaattt
    46201 tgaagtctta tatgatagat aaacttaatg gtagacgaat gggaatgaat cctacaggaa
    46261 gttctcgtag acaaaactac aaatttgcac caacttcaag gatgactaac acttatattg
    46321 ctgctggaga agatagacca gaagatatta taaagtcaat accagatgga ctttatgcta
    46381 aaaaaatggg tggtggttct gtaaatcctg taacaggaga gtttaatttt gcagtttcag
    46441 aaggttactt aataaaaaat ggagaaatac aagagccagt tagaggtgct agtttaattg
    46501 gaaaaggtag cgatatactt atgaatatag atatggttgg gaataacgta aaacaagctc
    46561 aggggatgtg tggctcatca tcaggaagta taccaactaa tgtaggccaa cctatgataa
    46621 gggtaaaaga aattactgta ggtggaagat aggagggata attgtggaac ttaaaaattt
    46681 taaagatata ttatttaaaa aggctctaag tgaaggtttt gaagagtgtg aaatatatta
    46741 ttatacagga gaaaatttaa gtataaatat atatgaagga gaagttgaga aatacaatct
    46801 tgataaatct tttggtttat cttttagagg taaggtaaat ggaaagatag gttattctta
    46861 tacagaaatt ttagatgata aagctgttga tatgttgata aaaaatgtaa aagatggagt
    46921 aaacactatt gaaaatgaag atgtacaatt tatatatgaa ggagataagc actataatga
    46981 tgtaaagact tattctaaag agctagaaaa tttagaggca gataagttga ttgatttagc
    47041 tttagaaatg gaaagagaga ctaaagctta ttcagataag gtggtaaatt tatctaaatg
    47101 tactatatct tattctgtat caagtaatgg tatatctaat actaaaggtc ttaatttaag
    47161 caataaaact aatatgctta tagggtttgt agttcctatc atagaagaca atggacaaaa
    47221 atatgatgga ataggatata atatagcaaa ttctattgaa gaaataaaac cttgtgagat
    47281 agcaaaactt ggtgttgata atgctttatc taaagtaggt ggtaagagta ttacatcagg
    47341 taaatataaa actatattgc ttaatgaagc tatggtatct ttgcttagca cattttctgg
    47401 gatatttaat gcagattcag ctcaaaaagg attatcttta ttgaaaaata gagaaggaga
    47461 catgatagca tctcctatag ttacaatagt tgatgaccca cttttagaaa atggtatggc
    47521 atctactcct tttgatgatg aaggagtagc tacatttaaa aaagaagttg ttttaaatgg
    47581 aaagcttata actttacttc ataacttaaa aacggctaat aaggcaggtg taaagactac
    47641 aggaaatgga ataaaatctt catattcatc acctataagc atatatccaa ctaattttta
    47701 tatagagaaa ggagataaat ctttagatga aattataaag gatatagatg aaggattgat
    47761 ggttactagc tttgcaggac ttcattcagg tgcaaattca gtaacaggag acttttcttt
    47821 agctgccaag ggcttttata tcaaagaagg aaaaaaagta tttccagtgg agcaaattac
    47881 agtagcagga aactattttg acctgttgaa agatattgaa gctataggag aagatttaga
    47941 atttccaatg agtagtatag gttcaccttc agttgttatt aaagagctat cagtagctgg
    48001 aaaagatgaa taattaaaaa gtaattttgt ggataaaagg tcatgttaat tttaattaat
    48061 atggcttttt attatttaag tagattttat tatctaagtt atatgaaaaa gttgtgatgc
    48121 atacaattaa tatcatacat taaatttaaa ggaggagata ttatgagtgg tgcacattat
    48181 gacatgcaca agcgaaatgg atattcaagt gatgagccat atccagagat aaaagtttta
    48241 ggtccaaata agtattatgc agaattattg atggatgact atgctggagt atcaagtgaa
    48301 tttacgagtg taaatcaata tctataccat aattttgatt tagatgaaac gcatagagag
    48361 ttaagtgaaa tgtggataaa tatatctata acagaaatgt tacatatgga gatattagca
    48421 aagactataa gattattggg tggaaatcct gtatatagag gttctacaag ttcttgtggt
    48481 gcatattgga atggtggttt tgtatgttat ggaaattcta tttgtaacag attaaaacta
    48541 gatttgcatt tggagcatgt ggctattaat aattattata aagatataag tcttattgaa
    48601 gacccttata taaaagctat attaaataga ataatcctag atgaaaaact tcatgtaagt
    48661 ttatttgaaa aggcaataga gaagtattgt aaataaattt gaaaatttag ttattcatca
    48721 ttttattcat atatataaaa taaccatcat acattttctt gaagggagtt gctgttgata
    48781 tgtattatgt atatgtgaat gatataaaca tagcagtata tgatttgaat ccgagtgcta
    48841 aaaaaactgt actttttata catggttggc ccttaggaca taagatattt gaatatcaaa
    48901 caaatatatt gccaaaactc ggatatagaa cagtttcaat tgatttaaga ggttttggta
    48961 aatctgatgc aacatcagga ggatatactt atagccaatt agcagacgat atttataagg
    49021 tggtacatgc tattggatta aaagacttta cccttgttgg tttttcaatg ggaggagcaa
    49081 tcgtacttag gtatatgagt ttatttaatg gatatggagt gtcaaaattg gtattagctg
    49141 cagctgctgc accatcattt gtacaaagac ctccagaatt tccatatggt atgacacgtg
    49201 aagatgtaaa taaattgata gcacaagcat gtactgatag accagaaatg gtaaccgatt
    49261 ttggcgaaaa agtatttgca tcaaatccac cagaaagctt tagaagatgg tttaatgata
    49321 ttggttttag tgcatcaggt attggcacta ttggaactgc tgtatcttta agagatgaag
    49381 aattatttaa tgatttaaaa tgtgtaagag tgcctactgg tatatttcat gggaaattag
    49441 atgaaatatg tccgtatgaa tttgcagttt ttatgaatga aaaaatagaa gattcaattt
    49501 tatatacttt tgagtatagt ggtcatgcta tattctatga tgagcttaaa ctatttaatc
    49561 aggaattttt acagttttta gaggaataga tatattttat tatcacttag aaaaagagta
    49621 atattatgtg aaaagattta agctaaattt tatgatgttt tttacactaa acaaataggg
    49681 ttttagaact tgtaactaag ttctgagacc ctattattat atattcctaa aatttgttat
    49741 aatttattcc ttgtcattct taaaaatgta ttaatatatt aacgaattta tattaaattt
    49801 ttatttatat tccaataatg tcaaactaac cgttttagaa attaaattat atgtgaaaaa
    49861 agatagctta cattatttta ttatggaaaa taatagtaaa tggtacaaaa tttgattttt
    49921 aagatttaaa acttttttaa aaagtgctac aatagaccaa attattactt tctatactcg
    49981 ttaaacagga ggtgggacta tgaaaaaaca taatatttta tttgtatcaa ctgatgacaa
    50041 aataaatata gatatatcaa aacaattgga aaatatattt ggagaatttt gtaatataga
    50101 taacttggta tatgtaaata ggataaatat tgaactgtca agctatgagc ttgtagtatg
    50161 ttcagacaat gatataaagg aatatataca taataatatt gataaaaata tacctattgt
    50221 aatagtacat cgtactataa atattgaaaa tataaatcag ataataagta tagaaaatga
    50281 ttctgatgtt atggttatag atgcttataa agagtcagca gatgaaactg ccaagataat
    50341 tagaaaacta ggattaattc atattaatct aataccatat tatccaggtt gtgataagtc
    50401 taaatgtgag attggaatta ttacgggaag cagaaacagc ataccacaaa acataaagca
    50461 aataattgac ataggtgata aggtaattga tataaataca gttatagaaa tctttacaaa
    50521 actaaatata tctatagata aactacatat tattaaggaa aaatacaatg aagatacagt
    50581 aagtggatat aggtattata caactatgaa taaaaccatg aagagttttt tggagattat
    50641 agatgaaggt attgcttctg ttgataaatt aggaaaattt atatattgca acaaagtatt
    50701 ttctaatcta gttggaatag accaaaatga aattatatct aataacttta tggacttatt
    50761 tagtgataag gttgtcaaaa aaatattttt tcaagaagat gaagttaatg atgaagtagt
    50821 aaatttaaat aacaaaaaac tgataattaa taaagtaaat gtttatgaaa ataatgaacg
    50881 aataaagagt attataagca ttaaaaatat aagtgcaatt caagtgcttg aggacaagat
    50941 tcaaaataaa cttcagtcaa aaggttttgt atcaaagtat acatttgaga gtgttgtagg
    51001 tgagagtaaa ataataaaag aaaaaattaa tattgcaaga aaaatagcaa ctacagactt
    51061 ttctgtgttg atacttggag aaaatggaac aggaaaagaa atatttgccc aggcaataca
    51121 taatgaatct ctaagaaaaa acaagccatt tgttgcagtt aatctatctt ctttgtcaga
    51181 tacacttatt gaaagtgaac tttttggcta tgatgaagga agttttacag gagcaataaa
    51241 aggtggaaaa atgggtatat ttgaaagagc acatacagga acaatttttt tagatgagat
    51301 aggtgatata tctttagatg tacagcagag attgctaaga gtattacagg aaaaagaagt
    51361 tatgagaatg ggcggaagta aaattatacc aatagatgtt agaattatag ctgcaacaaa
    51421 taaagattta aagaaaaaaa ttaaagaagg ttcttttaga gaagatttgt attataggat
    51481 taatgtatta catatagaga tacctagact tagagaacgt aaggaagata tacctcttat
    51541 aagtaagtat tttttagatg aaataaatag caacaaatgt tttacagagg aatcaatgca
    51601 agctttaaca ttgtatgaat ggccaggaaa tgtaagagag cttaaaaatt taatttatta
    51661 tattgatact atagttgagg aagatagagt agattacgag catttgccag aacaatttag
    51721 atttgaaaaa aacaatactt tagttaatga aaatttcgat tcaattatct tagatttcaa
    51781 acaatctaat ttctttgagg aaagtatatg tatattgacc agtatagaaa cttggaataa
    51841 taaaaatata ttattaggaa gaaataaact gcaagagatt ttaaaagaaa aagatatagt
    51901 tttaagtgta gaccaaatta gaaaaagaat agataagtta aagagccatg gtttactttt
    51961 atctggagta aaaaaacaag gtagttttat aactgatgaa ggaaagaact ttatatcata
    52021 tgtaaaaatt aagggagtga tataggggac taaaaaggca aaacacccta aaaaaatccc
    52081 tataatggat agaaataaat aaaattatct ttaagattaa gataattcaa aaatattgaa
    52141 taaataatct aagaagatta tttttaataa aacagtagta aatatcaacc ttactactgt
    52201 ttttattttt tgcaagattt attatacaat taaaatttat attttggcac atcaattgct
    52261 acataggaaa aatataaaaa attgtagaac ttggaggatg ttgttatgaa gtcagcttac
    52321 aaaggatatg aagcctatag ttatttagaa aaaggaaaag atatttcatc tattgagatg
    52381 tgtaaaggag ataaaagagt taaagaatac cttattgaat tagatgatat tcaagaagct
    52441 aaggttaaaa gtatagtgga tgaaaaaata tttatatcat tacatgaaca tccagtatta
    52501 tttccagaac acctagaaag agatatattt gaatataata gggaaggtaa acaaagatgt
    52561 gcttatgaag cattatcaag aggatattat gatgctatat ttgataattt aatggatggt
    52621 gtatgtacga taacatcaaa ttcagggtgg aaatgggatg atatattaat agatttaggc
    52681 atgagactat gtgatttagc tcatcaagat tttgttataa agtgtgaaaa agtagaagat
    52741 atatacagag ctaaaagtga aggtaaagta gcattgatac caacattaga aggtgcagca
    52801 ccaatagaaa atgaacttga tagaatagat atactgtatg gatttggtat aaggttaatg
    52861 ggtataacat acagcgaatc caatgcatta ggttcaggac taaaagaaga gaaagatgga
    52921 ggtcttacaa atttcggaaa aaaagctgtt gaaagaatga ataaaattgg aatggcaata
    52981 gattgctctc acgttggagt tcaaactaca cttgatgtaa tagaatatag caaggaccca
    53041 ataatattaa gccatgttgg ggcaagaagt ttatggaaca caaagagact aactccagat
    53101 gaagtattaa tagcatgtgc taaaaaagga ggggttatag gtgtagaagc agcacctcat
    53161 actacaataa cagagaagaa taaagaacat tcaatagagt catttatgga acattttgag
    53221 tatattaaaa atttagtagg aatagaccat gtagcttttg gaccagatac agtatatggc
    53281 gaccatgtaa aattacactc agtactagct gctaaatttg atataaaaaa aataacagga
    53341 acaaaagaac atcccaaagt agaatatgta aaaggtcttg agaacccaac agaaacttca
    53401 aaaaacattg ttagatattt agtaaagcat ggatatagtg atgaggaaat agagaaggta
    53461 ctaggtggaa atatacttag agtactaaaa caggtttgga aatagatata ttagaaatat
    53521 taataaaatt tgaggattta tagggaggtt ttataaatgg attatttaaa attagctaat
    53581 catccacttt tgtggatagc gagtacagta gcagtatcaa tagtagtggt acaatccctt
    53641 atatttgcta taaaatcttg caaagttggt aaaacaatgg gaataacaga taaacaaata
    53701 aagtcagctg ttaagagtag tgctatttca gctattgggc cttcaatgac tatatttgct
    53761 ggaatggtat cgcttatagt aactatgggt gggcctatag catggatgag attatcattt
    53821 ataggttctg taatttttga atcaatgtca gccggatttg gaacagatgc attaggtata
    53881 actttaggtt ctcctgaaat gacaaaactt gcatttacaa atgcagtttg gactatgata
    53941 ttaggttctt taggatggat aatattcaca ttactatttg gacataaact agataagata
    54001 accaatgtta tatctagtgg caagaaatca tttataccaa taatatctct aggagcaatg
    54061 ttgggttcat ttgcatatct aaatgctgat agggtgttgc gttttgataa tggaacaatt
    54121 gcatgtataa gtggaatggc tataatggta atattgtgta tgcttgaaaa gaagaaaaat
    54181 ataaaatggt taagagagtg ggggcttaca atatcaatgt ttagcggaat gataatagct
    54241 tcagtaatat aaggggggag ttttatgagt aaagtaaaat ctatatacaa tgaagaatat
    54301 ttacctttta tgataaggta tggaagacta acactatctt tgggtattat agcagcatta
    54361 gttcctggaa taattttgag ctttggattt ggtattatgc ctccaatatc agcattatta
    54421 gcaagtacaa tggcaattgt cagtatgagt gctccaaatt acataataga accagtttct
    54481 tattcaccaa tcttaggtat cccaggaact tatatgagtt ttctttctgg taatatatct
    54541 aatatgaggc taccatgttc aatagcagct caaaaggcag cagaagttga atcaggtaca
    54601 gaagaaggct cgataatatc tacaatagga atagctgtat ctattttagt gaatataagt
    54661 atactaacta taggggtaat tttaggagga agcgtactat ctaaaattcc agctgaagta
    54721 gtagaaaaat taaacttaat tttaccagct ctatttgggt cagtttttgg acaagtattt
    54781 ttacaagata aaaaattagg tctagtagca attgtaatat ctgtattgac tattatatta
    54841 tcaaagcaag gtataatacc acagtctcta gtagttctta tatgtgtatt tggtactata
    54901 ttaattgcaa gagctatgta taaagataag cttagtgact aattacaatg aaataatagt
    54961 gaattttcaa atatgaaatt taataatttg attttcaaat ataaaaatta cattaagatt
    55021 atttaaaaat tggtttttaa ggagggaata ttttgatata tgatttaatc ttaaaaaatg
    55081 gatttataat agatggtaca ggtaatcctg gtttttatgg agatatagca attaaagaca
    55141 acttaatagc aaaaatagat tctaagataa atagcaatac aaataaagag attgattgtt
    55201 atggaaaaat tataacacct ggatttattg accctcatgt tcatgaagaa atagtagcaa
    55261 tactagatgg taaatttgaa aagtttctaa aacaaggagt tacaacaact ataaatggta
    55321 attgtggaca ttctataaca ccatattctt ctgaaaatgt atatgaatat atgtataaaa
    55381 atggtctatt acttgaagaa gaaaaaaagt atcttataga taaaaataaa tgttggaata
    55441 attttacaga atattgtgac cttatatcta aaagtggtat ttctgtcaat atgggattct
    55501 tgttggggca tgggactata agatggagtg ttatgggagg ttctaaggat agacctccta
    55561 cagaaaagga aaaaaatgaa attacagata taataaatga tggaatgaaa agtggtgctt
    55621 ttggaatatc tactggattg gcatatatac caagtaagta tgctgatatt gatgaacttg
    55681 ttgatattgc aaggcagatt aaagagcatg atggtatata tacatctcat attagagatt
    55741 acataggaag atataatgca gtaaaagaag ccatagaggt tgggcagaaa agtggtgcta
    55801 gggttcaagt atctcactta agtccagttg aaatagaagc atttgatgag atattaaagg
    55861 ctaggtataa tggtgttgaa ataatggttg acacagtacc aagaagttct gggcattgta
    55921 tgaagaaaaa aagagtaatt caatttatta tggcaatatc atctagtcta tttgaattag
    55981 gtatagatgg ggtaatggat gctcttagaa atgaagaggg aagaacactt atattaaaag
    56041 aagcatttat acttggagat agggggagta taatacttct aaacactaaa gatattaata
    56101 tggaaaagaa atcaatacgt gaaatagcta cacaaaaagg aatagatgag gacaagttat
    56161 tactcgattt acttttagat ggagacgaag aacttatctt ttgtttagga ggaatgtata
    56221 gagctgattt cccagataaa ctacatgatg ataaaataat tgataatcca tttgttatgg
    56281 ttggttcaga ttgtttgttt agtgtgggtg gggatatgtc atggtttgaa ctacaaagaa
    56341 gaggagcttt tccaattttc ttcaatatgt acagaaagtc tggtgttaga ttggaagaga
    56401 ttgtaagaag agttacttca ttaccagcta ggcaatttaa aataaaaaat agaggtattc
    56461 ttaaagaagg gttgatagct gatatagcag ttattgacat ggataactat agttatccta
    56521 ggtcagaaga tattgatttt tcaaagccac aattactagc caatggtgtt gaatatgtaa
    56581 ttgtaaatgg aaaaattgcg ctagaaaatg gagagattac tgagaataaa tgtggtgaag
    56641 tattaaaaag ataaggtttt aaaaataata ggatttagaa agtaaataaa tatctaagta
    56701 agtaaagacg aagtttgaga tttatttaaa taaaaatcat gcttcgtttt tatttatagt
    56761 attaatattt attttaataa tcagataata tttatacttt atagtgaaaa aattaatatt
    56821 tttaatcatt aaattatgta aaaaattgga taaatagata actaaatcat ttgagaaaag
    56881 atttttagaa ataaaaaatg ataactaaat aaccccttta atatttatca aatagccagt
    56941 cataaatatt aaaaggggtt atttagttta taaaaattat ttgtaataaa gtaggatata
    57001 ttaataaata tgatagaata tatattataa attaaattta ttaatataag gggggaactt
    57061 tgagttctta tagatttctt acattataag aaccaatact ttatgcggga gttattaaaa
    57121 gtaacactta tagtaatgct tgttttgata aatgcttttt ttgtagcttg tgaatttgct
    57181 atggtaaaac ttcgtagttc taggattgat accatgataa ctgaaggtaa taataatgca
    57241 aaaagatgta aaataataaa agacaactta aattcctatc tttctgcatg tcaacttggt
    57301 ataacacttt gttcacttgc attaggttgg atgggagaat ctacagttaa agagttaatt
    57361 ttaccaatag taagtgtatt taatcttagt gaaagtatta tatatactat atcaatttcc
    57421 atttcttttt taattataac aatgatggag gttgtaatag gagagttggt tcctaaagct
    57481 ttagcacttt ataatacaga aaaaataatg ttgagtacat cttttttatt agtaggattt
    57541 tataagttaa catatccaat aatatatttt ttcaacttaa gtactgattt atttcttaaa
    57601 ccatttggat attcacaagc tgatgaggtt gatgaacccc atacaggtga cgaaataaga
    57661 ctattagtag aagaaagtta taaaagtgga cttatagatg aatcagagca aagattagtt
    57721 gataatattt ttgaatttga agaaaagaaa attagagaga taatggttcc tagaactgat
    57781 atggtatgta tatatgaaag tgattctgaa gaaaagatat tagctatttt aaaagaggaa
    57841 ggtgttacaa gatatccagt atgcagaaaa aataaagatg atatattagg ttttgtacat
    57901 ataagagatt tatacaatca aaagataaat gaaaataaaa ttgaactaga agaaatattg
    57961 agagatatta tatatatatc tgaaaattta actatagata aagctcttga gagaataaga
    58021 aaggaaaaac ttcaacttgc tatagttgtg gatgaatatg gtggtacatc tggtgtggta
    58081 acaatagaag atatactaga agagatagtt ggagaaattc aagatgaatt tgacaaagaa
    58141 gagactcata ttaagaaaat aggagcaggt tcttacttag ttgatggaac tgaatctatc
    58201 aactatatta ataagtattt tggacttgat atagagcatg atggatttga cagtataggt
    58261 gggtggatat cctatatgct tggttcaaat attaaagtaa accaaagtgt aacttttgaa
    58321 aattacaatt ttacaatatt agaattggat aaattgaggg ttgtaaaatt aatcataaaa
    58381 agtgtaatat aattatatca aaaaatattg gaggaattgt atgcaaaaag tagaaggaat
    58441 tatttttgac atggatggag ttttgtttga ctcagagaga atttctcttg aattttggat
    58501 ggagacgttt gaaaagtatg gatatactat gactaaggaa atttatacat cagttatggg
    58561 aagaaatcgt aaaggaataa tagaaggtct tacagatata tacgatagtt ctgtaccaat
    58621 aatagattta tatgacgaaa aaactaaaaa tatgattgaa tttatggaga ggaagggagc
    58681 tcctatcaaa ttaggagtca atgaactaat ctctttttta aaggaaaatg gatacaaaat
    58741 ggctgttgca acatcaacaa agagagaaag agcagtaaag agattagcta aggcaaattt
    58801 aaaagattac tttgatgcta tagtttgtgg agatgatgtt gtaaactcta aaccaaaccc
    58861 tgagatattc ttaaaagcag ctaaaaagat aaatgttaat ccccaaaatt gtattgtcat
    58921 agaggattcg cctatgggag ttgaagctgc atacaatggt ggtataagat gtataaatgt
    58981 accagattta aaagagccag atgagcaaat taaatcacaa tcacataaga tacttgaaaa
    59041 tttactggaa gttagagaat acctaaaaag tttgaactct aaagaatgtc acaatctata
    59101 atataaagta ttaattaaat aaattaaata taatttcttt gatttagaat aaatccatga
    59161 atatgagaaa aaataagtat aaacagctag gaggaatatt catggataat gtaggttctc
    59221 tattatattt tttatttaat tttttcttgt atggttttat aggttggata attgaaaact
    59281 tatatagtta ttataaacaa gggcattttc aagaagatgg atttttaaat ggtccattta
    59341 aaccaatgta tgcaattgca atgtcgatta taatagaggt ctcaagattt attgacccca
    59401 ataatatatt tgcattgctt ttattatgtc tagtagtgcc gacagtagta gaatatatta
    59461 caggaatact tatgaaagta tattttaata aggtctattg ggattattca gattataagt
    59521 acaatagtag aggaatagta tgtctaaagt tttctgtata ttggactata ttgacattta
    59581 ttggagttag atattttcaa gtgtatatag ttgatagttt atatagtctt ataatacctt
    59641 attggccaat tgttgtaact atacttttag taacattgtt tgtggatgaa attcttacta
    59701 taaaattttt gaggggaaat aaagatatag tagaaagata actttaaaaa tataagttga
    59761 atttagattg tagttaaaag aaagctatgt taaaatggtg aaatcattta aaattagctt
    59821 tcttttttat ttgataaaat tattttataa tatatctata agttgtaaag ttttttatta
    59881 aaattaaaat aaagtgtaga aaatggggag tgatttatga tgggtaaaat aagatttggt
    59941 attgttggga caagcaatat agcaaatata tttctaagag ctgcatctag ggttaaagac
    60001 tttgaattgg ttgctgtata ttctagaaat ttagaaaaag ctagagaatt tggaactcta
    60061 tatggagcaa gtattttttt tgatgattta gaacaaatgg ctaaatcgaa ggatatagat
    60121 gctgtataca tagcttctcc aaatgcatta cattcaaagc aaaccattac ttgtttaaaa
    60181 cacaaaaaac atgtgctttg tgaaaagtcg ttagcatcta atttaaaaga agtcaaatca
    60241 atgataaaaa cagccaaaga taataatgta ttgcttatgg aagctatgag aattacatgt
    60301 gttccaaact ttaaagcagt taaagaaaac ttatataaaa tagggaaaat aagaagattt
    60361 tttggaagct attgtcagta ttcttcaaga tatgataaat ataaaaatgg aattatagaa
    60421 aatgccttta aaaaagagtt gtcaaatgga gctttaatgg atataggagt gtattgtata
    60481 catcctatgg tgaatttatt tggtgctcca aaaactgtaa aggcagtttc tcatatactt
    60541 caaactggag tggatggaga aggttcagct gtatttcaat atgatgatat ggatgcgata
    60601 attcagtatt ctaaagtagc tgattcttat ataccatctg aaatacaagg agaagaggga
    60661 agtataataa ttgaaaagct taatctattt gaaaaagcta ttattaaata cagagatgga
    60721 agagaagaag atatatctat tccaaaagaa aaagcagagg aaaagcctag agaaatagaa
    60781 ggaatgtatt atgaacttgt agagtttata agtcttataa ataacaataa aatagaatca
    60841 aatataaatt ctcatgaaaa ttcaataata gtaatggaaa ttatggatga gataagaaga
    60901 caaagtagta ttgtttatcc agctgatagt atatagttta tattagaaaa tataagaatt
    60961 tataatatat attcttaaat aaatttaatt ttaagttaag gtaaggtgta tacttaacca
    61021 agtgttaaaa tgtcaaatta taagtatgaa aaagtgctaa aaagaaattt atttttggcg
    61081 tttttttctt tttttttaca attttgtaac agtatcaaaa aaagttttga cgattttatt
    61141 acaattttgt agttgaatat acatatcaaa actaatatga ttaataataa cataaatttg
    61201 aatattttta gaggagagaa aatatgagaa aattgatata ctttattaca ccttttataa
    61261 taggcgtagt atttttattt gggttagaca aatttcttga ttctaaaact gatgaacttt
    61321 tgagggagaa aaatctactt ccaattatgg atgatacttt aagtgatata aaagataaag
    61381 gggttacagc aaataatcat tttttaagag aaaaggatat tatgattttg gggtcatcag
    61441 aacttagtaa ttcaacaaaa caacatccaa agtactattt taatacaaat aggagtaaaa
    61501 ataaggtgtt tgcaattggg agagcctata ctcagacatt acaagatgct gcaatattag
    61561 gaagtatgaa tcctaatatt gataataaga aagttgtctt gttaatttct atgcaatggt
    61621 ttatggaaaa agacggagtc acaagtcacc attatcagtc tagattttca ccaatacaat
    61681 tttatcgatt tttagataat ccaaaaatat ctaaacaaaa taagattgaa tatgcaaaaa
    61741 aatcaagtaa gttattatgg ggaagtgacg agtataaagc agaagcatta tatgctaagc
    61801 tatatgagcc taagacattt cctgaaaaag ctgaaaaggt gcttttagag ccttattttc
    61861 aaggaagaaa gtattgtata gctcttaaag aaaagggtat tctttataaa cgattaatta
    61921 agctagataa aaagagagca acaaaaagaa aaagaccaat aaattggtca tatgagagaa
    61981 agaaagccat tgaagatgct aaaaaaagag ttggtaaaaa tccactaaat attgataagt
    62041 actattataa agagcatttt aaagatggta tagagcaata caaaggaaga gacaaagatg
    62101 taaatttatt aacatctaag gagtttgaaa attataaact gatgctaaat gtatgtactg
    62161 acttaggtat taagccagtt gttgtattaa tcccaagtat ggataaattc tataatttga
    62221 ctggtatttc tgaaaaagaa agaaatcagt actacgataa agcacaaaaa atagcagaaa
    62281 gcaaaggctt tgaagttcta aaccttaaag ataaagggtc agataagtat tatttaaggg
    62341 atgtaatgca tcttggtacg aaaggatggg tagatgtctg tgagagatta tttaaaatat
    62401 ttaaagaaca ataaagattt gttaactagt atttgtttaa ctatggttat aatattggcg
    62461 ataggagttc taacatttac tccatttaaa gaattgccat ttatttataa cggattttaa
    62521 aggagcattc ttatatgaaa attattgaag gaataaaaaa atattcaaat acagatagaa
    62581 cagcactaat gtgtaatgga gataaacttt cttataaaga tttaaatgaa tattcagatg
    62641 ctatatcagt atttctaaag gatgtataca aagaagaaga tactcctata gtaatttatg
    62701 gaaataaaga aaacatgata atggcatgta tgataggagc tttaaaatca ggtagagcgt
    62761 atgtgcctct tgatattagt tttccaattg atagagtttt tgaagtcact aaagagataa
    62821 aaccaaaggt attatttaac tttagtgatg aaaaagattt tggagatatc aatgtaattg
    62881 atatggataa attaaattat ataataaatg aatatcaggg aaaatcatta gataaagaaa
    62941 actgggtaaa agatgatgaa aatgcatata tactatttac ttcaggaagt acaggaaagc
    63001 caaaaggggt tcaaataagt tcaaacaatt tagatagctt ttcagattgg ataagcccat
    63061 atctaaacat agatggaagt gaaaaagtaa taatgaacca accagcatat tcttttgacc
    63121 tttcagttac aacaatatac cctggtctta tccatggagc tacattattt tcaatatcaa
    63181 aagatgtatt agcagactat aaagaactat ttagacaatt tagtatctca gatatagctg
    63241 tatgggtgtc aactccttct tttgcaggag tatgtataac tgaaaaagag tttaatagta
    63301 aaatgcttcc taaccttgag tcaatgatat tcataggaga agcactttca aaaaatctta
    63361 ctaaagaact tatgagtaga tttccaaaca caagaattat caatggatat ggaccaaccg
    63421 aggctactgt tggtgtaagt gtaaatgata tgactcaaaa ggctatagat gatgaaaaaa
    63481 gtcttccagt tggataccct atgtcaaatt gtaaaataaa aatacttgat gaagatggaa
    63541 atgagcttaa agaaaatgaa aagggtgaaa ttataataat tggaccatca gtttctaaag
    63601 gttacttcaa taataaagaa aagactgatg aagtattttt ctatgatgaa atagatggtg
    63661 taaagtggag agcttataaa actggagata tgggatatct tttagatgga aatatatatt
    63721 attgtggaag aaaagatttt cagattaaat taaatggatt tagaattgaa atagaagata
    63781 tagaaaataa tttgagaaaa gtacataatg taaaaaatgc agtagtttta ccagtatata
    63841 aggacgaaaa aattgcttac ttaaaaggta ttgttgagtt aaatgaaaag aatgatttaa
    63901 gtaatattaa aaatgggatg ataataaaaa aggaacttgg aaagtatata ccttcatata
    63961 tgattccaag aaatatttca ataatctctg agtttccaac aaatatcaat ggtaaaatag
    64021 acagaaagaa acttatggag gaaatttaat gattttttca caatatggag attattttta
    64081 tctttatata ttattattaa catcaatacc agcagtaatt ttaggtctaa tgggcaagaa
    64141 tataaagtat tatggtatgt tggcaagttt gtttatgata ttcttaatag taggaattga
    64201 tgtccaacta aagtacttgg taatatttat tattcttgaa gtaataatag ttaaaggata
    64261 tgaatatgta agaagaaaaa ctaaaaataa atacatatat tggggattct tgtttgcatc
    64321 aatgctccca ataataataa ataaaatatc tccagttact tcttttggaa taattggatt
    64381 tattggaata tcatacttaa atttcagaac tatacaaatg gttattgaaa tttatgatgg
    64441 agctataaaa gaagttaaaa tttcaaagat gctttatttt atgttatttt ttccaacttt
    64501 aagttctgga cctattgata ggtcaagaag atttgaacag gacttggaaa aacaaatctc
    64561 tagaaaagag tatatagaag aatacttact gccaggaata aaaaatattg tcatgggagt
    64621 aggttataag tttgttatag catttttgat aaatacatat tgggtatcaa gaatacctaa
    64681 agatatgagt tttataaata ttttaagtta tatgtacgca tatagtttat acctgttctt
    64741 tgattttgca ggatatagtt tatttgctat aggtacagga tacatatttg gaatacaagt
    64801 acctataaac tttgacaagc catttatcag taaagatatg aaagagttct ggacaagatg
    64861 gcatataagt ctttcaagat ggtttggaga ttatattttt tctagatttg ttatgtcatc
    64921 aatgagaaag aaaagattta agaaaaggac tactgctgct catgtggctc agatgataac
    64981 catgataaca atgggatttt ggcatggtct aacttggtat tatgttgctt atggtgtata
    65041 ccaaggattg gcacttgtgt taactgatat atatcagcga aaatctaaat tttataaaaa
    65101 acataaaaaa gataaatggt tcgagagagt gcagattttc attacattcc atattgtgtg
    65161 ttttggattg ctgatattct caggttattt agcttaattt taaggaggaa aatttatgca
    65221 agaaacagtt atagaaatat ttgaagatgt attagggact gatgagataa gagaagattt
    65281 agaccttaat ttatttgaga cggaactttt agactcatta gctataattg aagttttact
    65341 tgaaattgaa aatagattag ggatagagct tcaaccaact gatttagaga gaaaagatat
    65401 gtctactgta aataatttag taaaattctt ggagacaaga aaataaatta gtagttagac
    65461 tttaaatatt aatttataaa ataaaatttt acaaagtcca tctgatacta atatttgatg
    65521 gactttttta atgataatac aaagtctttt gaaaataact ttaattaagt aaaaaattta
    65581 atacataaat atgatttcat aaatcttagg tatcattgta atattatagg agaaaaatat
    65641 attatattta ggaggaaaat gaatgtcttt aaattcgata gatagaaaaa aagaaatact
    65701 taaattatta gattcaaaag ggaaggtaaa cactaaagaa ttagtaaaaa gattagaagt
    65761 atcatcagaa acaataagaa gatatttgga agaattagaa aatgaaaata aattaaaaaa
    65821 ggtatatggt ggtgctataa aaataaatta tggagtgatt gaaccagaac atataaagag
    65881 aaacaatact aacctagata aaaagcaaag tattgctaaa tatgcagcta agtttgtaaa
    65941 tgacaatgag tcaattatca tagatgaagg tactaccaat ttgcagattg tagattatat
    66001 aatatctgta aacaatttaa aaattattac aaactcttat ccagttctat caaaattgat
    66061 ttcttatgag aatcaaggtt tgtttgatgg agaagttata tttattggtg gaaaagtaaa
    66121 tttaaaacac caaaggacag catgtccaat gtcaatagat actatgaaaa acttatacgt
    66181 agataaagct tttatatcta gtgaaggtat acttgatagt tttggaatta gtagtataga
    66241 ccaaaataag gcattgttgt caaaagaata tataaaaaat tcaaaggaaa gttttgtatt
    66301 atgtgatagt tctaaaatag gaattgctag tatgtataag atagcagatt tagaggaatt
    66361 agattttgtt atttgtgatg tagaacctcc gaatgaatgg aaggataagt taaatgagat
    66421 aaatataaca tggatagaag caaaataaac ccttagtttt atataataca actatgctca
    66481 atacaactaa attcataaaa tatagtcagt tcatagatta agattagaaa gtaaatttta
    66541 gattgtaact taggatttta tgggaatatg atacaaaata aaaacaacta aatacaacta
    66601 aaaacagaaa tttaatatat atttaaccta aatatgatgt ataattaaca tatgaaagtt
    66661 atactttaaa agtaaaaaca attaaaaaca caaaaattct aaaaagaatc tatggaggct
    66721 ataaatatga aaaagatata tggagaaaaa ataaaagcag tagtattcga ttgggctgga
    66781 acaacagtag attatggatg ttttgcacct cttaatgtat ttatagagat atttaaaaga
    66841 agaggtatag atgtaacaat ggaagaagca agaaaaccta tgggaaaatt aaaaatagac
    66901 catataagag aaatgtgtga aatggataga ataaagaata tttggtcaga taagtttggt
    66961 aaagtgccaa ctgaagatga tgtaaatgaa ctttatgctg aatttgagcc aatgttattt
    67021 gagacattag aagagtatac gactccaatt cctcatgttg ttgaaactat tgaaaagttg
    67081 agaaaaaatg gattaaaaat aggttctaca acgggttata caagagagat gatgaacata
    67141 gttgaaccaa atgcagcaaa gaaaggatac tcacccgatt ttcttgtaac accatcagaa
    67201 gtatctcaag gaagaccgta tccttggatg tgctataaaa atgctgaagc tttaggggtg
    67261 tctcctatga gttctatggt aaaagtagga gatacaataa gtgatgtaaa agaaggcgta
    67321 aatgcaggaa tgtggagtgt tgctgtaata aaaggttcaa gtgagttagg gcttacacaa
    67381 gaagaagtag agaacatgga taaggaagaa ttaaaagcta agatgagtat agtttcaaag
    67441 aaatttaaag aggcaggtgc acattttgta atagaaacta tggctgagtt agaagatata
    67501 ctaataaaaa tagagaatga aactattaaa tcagattttg taccagaaaa tgattacata
    67561 ttacttacac caggaccact atctactact aaatcagtaa gagcatctat gttaaaagat
    67621 tggtgtactt gggatgttga atataataac ctagtacaag atgttagaag aagattagta
    67681 tctctagcaa cccaaaatac agataaatac acaagtgtat taatgcaagg aagtgggact
    67741 ttttcagtag aagctataat aggaagtact atttcaaaag atggtaaact tcttgtaatt
    67801 gcaaatggag catatggaaa gagaatgaaa gatatatgta attatcttaa catacaattt
    67861 gttgactgca catttaaaga tatagaagca gtggatttaa atgtagtaga aaatttatta
    67921 aaagaaaaca aggatataac acatatatca atggttcact gtgaaactac tacaggtaga
    67981 ttaaatccta tacaagaggt agggaaactt gcaaaagaat ataacaaaat ttatatagtt
    68041 gatgctatga gtagttttgg gggaattgag attgatgttg aagattttaa tatagatttt
    68101 ttagttagta gttctaacaa atgtatacaa ggagtacctg gatttggttt tataatagct
    68161 aataaagaga aattatctaa atgtaaaggt atagctaaat cattatctct tgatgtgtat
    68221 gcgcaatggg agactatgga gaaaaataat ggtaaatgga gatttacttc acctacacat
    68281 gttgtaagag ctttttatca agctttatta gaattggaag aagaaggttc tgttgaaaaa
    68341 agatatgcta gatataaaga aaatcagttt acaatagctt caagattaaa atctttaggc
    68401 tttgatactt tggtgaatga taatgctcaa tcacctgtaa taacaacttt cttatatcct
    68461 aaaaatgcaa agtttgaatt tatggaattt tatacttatt taaaagataa tggatttgtt
    68521 atctatccag gtaaattgac tgatatagat acatttagaa taggtagcat aggtgaagtc
    68581 tatccaactg atatggagag actagctgat gtaatagaaa aatttatcaa tagatagaag
    68641 aagatagtgt cttgtaggtt acagaatagt gcgcaaagcc tactccttta ggtgtgtgag
    68701 atgatagcac aaacagtgta taaaaagtat tgacagtaga atttaaagac ttatatttta
    68761 gaagtttata caatgaatat ataaacattg agtaataggg atatatttta tttaaaccac
    68821 aatctaagaa attagattta tagtttaaat aaagcatttc cctattattt ttatgcctat
    68881 ttatagaaaa taaatgatag ttgctttatt aagtattatt atcatacttg acaacgtaac
    68941 aatgttttgt tatattaggt ttaaagataa aatacggtaa agaaaggata tcattatgaa
    69001 tgaagaatta agttcactaa gatttgatgg tgttggaaaa tgtaaaggtg gaatttttga
    69061 taatgttaat atatcaggaa caggcaaaat agaaggaagt gtaaaatgta gtaaatttga
    69121 ttcttctggt tcagcaaaag tttttgggac aacagaatgt aatgaatttt ttacatctgg
    69181 tttaagtaaa gtagaaggaa acatactggc atcaaaagtt gagatatcgg gacttcttag
    69241 atgtacagga aatattaatt ctgttgaaat tgatactagt ggaattatta atgtagaagg
    69301 aagtataaaa tcggatatta ttcatggaga aggatattta aaagcaaatg agaatataga
    69361 ttgtgaaaat atcaatttaa gtggaatctt ggagtgtaat ggatttttga attgtgaaga
    69421 agttaatatt agtttaaatg gaacttctaa ttttaatgaa gttggagcaa gtagtataaa
    69481 tataaaaaag gggcatggag attattgcaa aaaatttcgt atgctagaaa aaaagcatat
    69541 ctctagtaga taattatgat gttggaaata ttgaatacag tgataattta tcagtttcag
    69601 ataattctac tgtaaaaaac agtataaagg tgggctagaa gagctatgga agaattaata
    69661 tctaagaaag atttattgag caaaactaat atatcttatg gacaattata tagatggaaa
    69721 agaaaaaaca taataccaga ggaatggttt ataaaaaaat cagcttttac aggtcaagag
    69781 actttctttc caagagataa aatactagaa agaatagagt taatattgtc tatgaaagaa
    69841 gagacttctc ttgattaaaa aatagctatg cacacttgtg actttagtca tgagttagta
    69901 gctaaaatag tcagcatata gggaaactta tatgtagaga taggatagaa cctatccaat
    69961 actacttgaa ttgctggaaa cccctaaagc taaccaaact aaaacataag gatgaaataa
    70021 acctaagtgt gaaagttacg aaagtagaaa aaattggtta gatggtataa ggttaaatcc
    70081 taagtacttt aaaatgggca atcagcaggt aagctccgaa taggaaaaac ttcaacgact
    70141 attcctcttg agggaagtac actacaagct atcggtagtg gaagtgggta gaccttaacg
    70201 gataatgccg aaggataaga tatagtctgt gcttgcatga aagtgtaaga agttcataag
    70261 agaactgcat aggtagtagc gaacttatgt gaacgacaac ctcaaaaatg ataaaagtta
    70321 agatttgcgt ttcttacgta tgttatttaa aaataaatat aaaaacctta agaatacttg
    70381 ttttaagtaa ttactatgtt aatattatgt tgggtgatga aaaatggaag tgactcatgg
    70441 tagagggtat gtgtatttag tacaatacca tatagtatgg tgtgtaaaat atagacatcc
    70501 agtattaaca aatgaaattg aagaagattt aattaatata ttaaacaaaa tagctgaaga
    70561 taataaattt caaatattag aatgtaatag tgataaagat cacatacatt tactaataaa
    70621 ttgttcacca caacactata taccagatat aataaaagca ttaaaaggtg tatcagcaag
    70681 gctgttaatg aaaaaacatg gagaacagtt gaaaaagaaa ttatggggag gacatctttg
    70741 gaatcctagt tatttcgttg ctactgtatc agaaaataca gaagaacaga taagaaaata
    70801 tattaataat cagaaaaaac aagtaaagtg aggtggcagt agtggaaaaa gcttataagt
    70861 ttagaatgta tccaaataaa aaacaacaag agttaattaa caaaactttt ggatgttgta
    70921 gatttgtata taataagtat cttgctaaaa gaatagaagt ttataaaaac aataaagaaa
    70981 cttttactta taaacagtgt agttctgatt taaccaattt aaaaaaagaa ttaaaatggc
    71041 ttaaggaacc tgacaaattt tcactacaaa acgccttaaa agatttagat aatgcatata
    71101 aaaaattctt taaagaaaaa gcaggatttc ctaaatttaa atcaaagaaa attaatagat
    71161 tttcatataa aactaacttt acaaatggaa acattatgta ttgtggtcaa tatataaaac
    71221 tacctaaact tggtatggtt aaggtaaggg ataaacaagt acctcaaggt agaatactta
    71281 atgctactat atctaaagaa ccaagtggta gatattatgt atcattatgc tgtactaatg
    71341 tagatattga agtatttgaa aatactaata atcagatagg tttagattta ggaattaaag
    71401 aattttgtat ctcaagttgt ggagacttta tagaaaatcc taagtatctt aagaagtcat
    71461 taaacaaact tgctaaatta caaagagaat tatcgagaaa gacaatcggt agtttaaata
    71521 gaaataaagc aagattaaaa gttgcaagac ttcaagaaca tatagctaat caaagaaatg
    71581 attttctaca aaaactatct actaaattaa taaaagagaa tgatattatt tgcatagaag
    71641 atttacaagt aaagaatatg attagaaatc gtaaactatc tcgcttaatt tctgatgtgt
    71701 catggtcaga atttgttcgt caattaaaat acaaggctaa ttggcatggt agacaaattg
    71761 taaaggtagg taaattcttt gcaagttcac aaatatgcaa taaatgtggg tataaaaacg
    71821 aggaaatgaa ggacttaaat ataagagaat ggatttgtcc tagttgtaat gaaactcacg
    71881 atagagatat aaatgcaagt ataaatatat taaaagaagg attaagacta ataacaattc
    71941 aaaataaata acttatgtaa gaaccgtagg aactacgggg atagcctgtg gagaattagt
    72001 aagacatatt ttagtatgca aaattctatg aagcaggaac cctgtgactt tagtcatggg
    72061 aggttcagga tattgcaaat atgtttatga aaaaggatat taataaagaa tttgacattg
    72121 attttattct cagtaaagaa gttatatgta attataccaa agaaatattt caaaatttat
    72181 ataaaaaaga acaaagcata ggtaaaaaag aactgcttat attgagcata atagagaagt
    72241 ttttagttaa atctataata acttttgaag agttaaaatt gatagtgaat ataatagaag
    72301 aaaattttac atcaatatac aatgaaagtg gaaagattta tttgtttaga aagtttggag
    72361 ttccattcgt agtaggatgt ctggattata aaaaagtaaa gtttgaaaaa gatgtagtta
    72421 agattattga ggttgacttg acaaaagaaa taaatgatat aagtataaag ttatcatagt
    72481 gtattatctt atataatgta ttataataat ctacttaaaa tacactttat ttaaatattt
    72541 agtttaaaga tgagatttta tgtgagtgtg taaaagttaa agatagattt aagtaggtta
    72601 tagggattag taaacaataa aatgaaccat gatgaactac tataaattgt atttaaacta
    72661 cattttctac ttatagtaat atcaatggtt cattttattg ttattaagat aatacagtat
    72721 ttataaaaat acttgatata aatatagtaa attgtttaaa tagaattatt gccttaattt
    72781 attcaaataa tgagttttta aaatctaaga aatatccttg tggatgaaga catacaggac
    72841 aagctttagg agcttcttta cctatatgta tatggccaca atgtgtacat atccattgta
    72901 tatcttcatt tcttctgaat aaagtacctt cttctaaatc agtagcatat ctaccaaatc
    72961 tatcaccatg aactttttct atttcagcta tattttcaaa taacttagct atagattcaa
    73021 atccttcttc acgtgctgtt tttccaaagt ttttatatac atcatcccac tcttgaagct
    73081 cattatgttg tgcagcttta aggtgttgtg ctgtcttgtc atataaatct acagggtatg
    73141 ctcctgatat acttatattt tcaccagcaa attcttttaa tttagaaaag aattgttttg
    73201 catgtgattt ttcttggtca gcagtgtaat taaataaaga ctctattata tataatcctt
    73261 ctttttttgc tacagatgca gaaatattgt atctatttct agcttgactc tcacctgcaa
    73321 aagctcttaa taaattttct ttagttttac tatcttttaa attcatattc tacctctcta
    73381 tcacaataaa tttttacaat atttgattct ttttatttat tattccaata ggagatataa
    73441 ttatgctcaa tttagtcagc atttttaaaa ttatcattaa ttttttttaa aattctaaga
    73501 aattcttgtt tttcattttc tgtaatatta tggtatgccg tctctttaac ttgtgttgag
    73561 atgctattaa aagtatcttc tataagcagt gccttttttg taagctttat atacgtgata
    73621 cgtttgtcat ttgtacattt ttgtctttct aaatatccta ggtttataag tttattaacc
    73681 aacactgtaa cagtggattt atcttttcct attttagctg caatttcttt cattgtaagt
    73741 attccattat tttcataaag tgcagttaaa atatttccat gagtaggtat taactcacct
    73801 aaaccatttt tactgagttt attttcaatg aatttaatca tactagcctt tgttttactt
    73861 atgaaatgta caatatattt atcttccata aattattctc caatctttaa tctatttaaa
    73921 ttagtttgac ataaaactat ttataaacta taattagttt aacataaaac taaatgtatt
    73981 gggaggaaat ttatgaataa aaaatttaaa tcactatata ttacagaaga agaaggaaat
    74041 tttagaagaa atatagttga aagagaagtg gatacattgc cagaaggcga agttctaata
    74101 aaagtaaaat attcttctct aaattacaag gatgcacttt ctgcaaatgg taataagggt
    74161 gttacgagaa agtatcctca tacaccagga attgatgcat ctggtatagt agaatctagt
    74221 agttgtgatg attttaaaat tggagaagaa gttttagtaa caggttatga cttgggaatg
    74281 aatacttctg gtggtttttc agaatacata agagttcctt ctagttgggt agttaaactt
    74341 cctaaaaatc tttcactaaa agaagcaatg gattatggta cagcagggtt tacagcagct
    74401 ttatctgtgt ataaatttat agattctgta gacaagaata tgggagatgt attagtcact
    74461 ggtggtactg gaggagttgc tgtaattgca actaaaatat tgattaaatt aggttattca
    74521 gttgtagttt caacaggaaa attagaagaa caaaaagaag tattactaaa cttaggtgtt
    74581 aaagatgtta tacataggtc agaagtggat gataactcag gaagacctct gcttagacca
    74641 agatgggctg gagtaataga tacagtagga ggaaatactc tagcaacagc aataaagaca
    74701 actaattatt gtggagcagt aacaacttgt ggtaatgcag gaggagttga ttttacaagt
    74761 tcagtatatc catttatctt aaagggaatt actttatatg gtatagattc tgtggattgc
    74821 ccaatgaact taagattaaa aatatgggat ttattatcaa atgaatggaa attagatgat
    74881 ttagatgata tggtaaatgt agtatcattg gaagagttaa atgatagtat agattcaata
    74941 atggcaggaa aacatgttgg aagaacagta gttgatttag aaaaaatata ggtgaataaa
    75001 tttataatta ggattatagg ttttaagcaa aaatgagata cttgaaattt acttgtgagt
    75061 atctcatttt tttatgtaaa aagatttatg tagatgtttg tgtaaaaaga ttattcatga
    75121 acaattaatt caaatacatc tggtcttgaa tagtgacctt ctgaatcaaa atctagtcta
    75181 ctaagtacta ttttttctag gtctaaatca gctattagca tttcttcttt attgaaaata
    75241 ggacctgcta catattttcc aaaagggtca actatacaac ttcctcctgg acacataatt
    75301 tctggttctg catctagctc tttatagtag ttaaggtcag ttggatacat atttttttca
    75361 acatattgat tgcatccaat tacaaaacat cttccttcta aagcaatatg cttcatagtt
    75421 gcttgccact cttctcttga atctgcagta ggggctatgt aaagttttac acccttagca
    75481 taaagagctg ttctagctaa tggcatataa ttttcccaac aaatcaacga acccattttt
    75541 ccatatggag tatcaactac tgtaagagta cttccatctc cttctcccca aatacatctt
    75601 tctgttcctg taggtttaag ttttctgtgt ttaccaatta gcttaccttc tggagagaaa
    75661 aataagtttg tgcaataaag ggtacaattt atatcattac catctctttc tgttataccc
    75721 attgatagat atactcctgc ttcttgtgct gcttttccta ataaatctgt tgtagcacta
    75781 ggaactggta cagaattatc atagtatctt ttccaatctt ctctaccttc cattgttcta
    75841 cttccaacaa caaatccaaa tgaaagacct cttggatatg caggtatgaa tgcttcaggg
    75901 aaaactacga tatttgcccc ttttctacca gcttctttaa ttaatcctaa tgctttttca
    75961 acagtttttt ctaaatccat tatgactggt gacgcctgga caacagcaat tcttacattt
    76021 ttattttcct tgctcataaa aacctcctaa aaaatattta aattttttgc attgaataga
    76081 ttacatgaga tgttatttga gaactaagat acttatattg tgaataataa tgtaactgta
    76141 tatttaaaat aatacatcat gatttaaaaa attacaattg tacagatata attgagttta
    76201 gatatttaaa tttaacaact tataatattt tttcaattag tataaagcct attaatttgt
    76261 cttatttaca atgaatagta tttacttctt ggcaaacaat aattaaaaat aaaactttta
    76321 tatgattaaa ctctaaagaa aaagtttata aagaagataa tataattaag cagaaaataa
    76381 tgaaaaaaat ttattaaagt agacaacgta aggagaattt tatgaaagaa aaaatttatt
    76441 acaatggaaa tatcatcaca atggaagatt caatatgtgg agatgctatt cttataaaag
    76501 acaaaataat aaaaaagata ggaacaaaag aagaagtatt tgctttgaaa aataaagata
    76561 cagaaattat agatttacaa ggtaagacat tgatgccatc ttttatagat tcacatagtc
    76621 acttgatagc ttttgctact actctaaagc tagttccttt agaagatgca accagtttta
    76681 aagacatagt aaaaaaaata caggatttca aagagtcgaa taatataaaa aaaggagact
    76741 ggataattgg attttcatat gataataatt ttttagaaga aaataagcat ccagataaga
    76801 gtgtactcga tagtgcatct tcagaaaatc caatactaat ttcacatgct tctggtcata
    76861 tgggagttgc aaatacattg gggttaaaac agcttggtgt taccaatgaa acacgtgacc
    76921 cagaaggtgg acatataggt agagtagaag gcagtgaaga accaaatgga tatttagaag
    76981 aaaatgcatt ttttaatgta gcatctaaaa taaaacaacc atcatcaaat gagattttta
    77041 attcaattga aaaagcacag aacatatatc taagctatgg tattacaaca gctcaagaag
    77101 gtttaatgga agaaaatcaa tttaacattt taaaagcaat ggcaaatcaa aataaattaa
    77161 aaatggatgt tgtaggatat gtaaatttga aaaaaagtaa gtctgtagca gataataata
    77221 gggagtttat aaaaaaatat ataaatagat ttaaaatagg aggttataag attttcttag
    77281 atggttctcc acaaggtaag acagcatggt tgagtagacc atatgaaaat tcagatgatg
    77341 gttattgtgg atatcctata tacaaagatg aagaagtaga aaagtttata gatatttcac
    77401 taaaagaaaa gatgcaactc ttaactcatt gtaatggaga tgcagcagca gaccagttga
    77461 tagatgcgtt tgagaaagtt ttaaatttaa aagagcaaag tagtgaaaat catataagac
    77521 cagttatgat acatgctcaa acagtaagag aagaccagat tgatgatatg aaagtaataa
    77581 atatgatacc ttcatacttt gtagctcaca catattattg gggtgatatt catatcaaaa
    77641 accttggaga agatagagcg tttaagatta gcccacttaa aacaactata gaaaagggat
    77701 taatttacac tcttcatcag gatacaccag taatagcacc taatatgctg gaaacagtat
    77761 ggtgtgctgt caatagaatt acaaaaaaag gtatacaaat aggagaaaat gaaaagataa
    77821 gcccattaga tgctttaaag ggtgttacta taaatgcagc atatcaatat tttgaagaag
    77881 ataaaaaagg ttcaattaaa gaagggaaat tagctaattt gataatctta gatgaaaacc
    77941 cacttacaat cgaccctatg aaaataaaag atattaaagt attgcagact ataagagaag
    78001 gagaagtatt gtattctttg aagtagatga aagttagata ttttataatc tataaaaaat
    78061 agttttatat aataagttag gtagaattaa ataagattac agttaaatca acaattatat
    78121 ttttattaaa ttttaattat tgtattgaag atttataagt ataattttat tgattctacc
    78181 tttttaaata actgtcgatt tattaaagtt atctatgtac tatattaaac aattaagtag
    78241 ttattaattg aacttattag tttattaaaa aactatgtat attactttgc agtaagactt
    78301 tggaactaaa tctataatag caaatttaaa tttattaaat agattacctt ataacatttc
    78361 atataaaata tattgtgaat ctgagaaaat tactattttt ctttgagtat tttttgtaag
    78421 cagttctaaa agattcaatt tcatacctat tacttgaatc attcattaaa ataattctaa
    78481 tttaaaaaat aattctatcc aaataataga tatataggac tatgttataa tataaaaaaa
    78541 tttatataaa agttaataac tatacaagat tgataaagaa aaagtcaaaa tagataattg
    78601 attagcaaaa tagatataat tataatgaag ttaaatctag caatattctc ctaaaattaa
    78661 attacattta tttaattaaa tataatttga caatggacaa aaaagtgagt aaaatataaa
    78721 taaaataaaa ataacaggtt cggataaaga aaaaggtttc ttaacctcaa gcataaagta
    78781 tattatctaa gttatttgag attaagagat aaatccaatc ttaggtactg aggaggaaaa
    78841 aatgcttgat ataaaaagaa taagagaaaa tttagattgt ataaaaaaag caatggaaag
    78901 aaggggcgaa aaagacttta accttgatga agtagttaag ttagatgatg aaagaagaaa
    78961 gattcttcaa gaagttgaag ttatgaaaaa tgaattaaat acagcatcta aaaatatacc
    79021 aaatcttata aaagaaggta aagatgtaga aaatgaaaag attaaactaa aagagttatc
    79081 tgataaaatt aaggtaatag accaaaatct taaagaagta gaagataaga tggaatacct
    79141 attaatgaga ataccaaatg taccacatcc agaagtacca caaggtgaaa ctgatgaaga
    79201 taatgttgaa gtaagaactt ggggaaagac tactaccttt gattttgaat ctaaggctca
    79261 ttgggaaatt ggaacagaat taggtatact tgattttgaa acagctagta aaataactgg
    79321 ttcaagattt actctttaca aaggattagg tgctagatta gagagagcat tattaaattt
    79381 ttacttagac acgaatacaa aagtaaatgg atatacagaa gttattccac catttatggc
    79441 aaatagaaat agttttttag gtacaggaca gttacctaag tttgaagaag atatgtttaa
    79501 aatagaaggt atggactatt ttatgattcc aacatcagaa gttcctttaa ctaatataca
    79561 tgctaatgaa atacttaaat ttgaacaatt accgataaat tatacagctt atactccatg
    79621 ttttaggtca gaagctggtt ctgctggaag agatacaaga ggtttagtaa gacaacatca
    79681 gtttaacaaa gtagaaatgg ttaaaatagt tgcaccagaa gaatcttaca atgagttaga
    79741 aaagcttact aacaatgcgg aaactatgct acaattattg aatctgcctt atagggtagt
    79801 aaagatatgt acaggtgatt taggatttac ggcttctttt aaatatgatg tagaagtttg
    79861 gatgccaagt tacaataggt atgttgagat ttcatcttgt tcaaactgcg aagatttcca
    79921 agctagaaga gcaggaataa gatttaaaag agataaagat tctaaagctg aatatgtaca
    79981 cacattaaat ggctctgggt tagctattgg aagaagtgtt gcagctatat tagaaaatta
    80041 tcaacaagaa gatggttctg tggttgttcc tgaagtttta agaccatata tgggagtttc
    80101 tgttattaag taagagttaa attatgtatt tttaagcaat acaataatat agaggtaata
    80161 aaaaataatt aatataaaag tgatacaata atatagaagt aataaaaaaa tagttaatat
    80221 aaaagtgata caataatata gaagtaataa aaaaaaataa ttaatataaa aatgtgtaca
    80281 aactaatgaa tattgatata gtaagctatc atatgaaaga gtatgtacac atttttttta
    80341 ctaatttttc acaatatgaa cataaataat ttacaggaaa ggaatctttt atgaaaattc
    80401 aaaatactga gtggggatat attgaatgga aacatactta tgatgaaaat aatccaaaac
    80461 aagctatgaa tatatacatt gcagtaacta tgcctggtaa gaaacatttt aatcatgttc
    80521 attatggtca agagcagatg atatatattt tagagggaga gggtttgtat attataaatg
    80581 gagtttggaa gccattttat caaggtatga ttttttatat agaatctggt agcacacatg
    80641 aaactataaa tacaggtgat agagaaataa aagaacttat tgtttcaaat aatgtagatg
    80701 atgtaggaga gagtgaagtt atagatatta atcctaataa ttatttaaag aagaccttga
    80761 taaattatag tgaaagtaca ttaaatttgt atgcagcagt agaatcaatt cgtggtcaat
    80821 ttatagaccc gtttaaaatt ccattaatta tatatgatga ttcatggaat atagttttaa
    80881 aaaatcctta ttttccactt ttttgttttg aaaaatgtaa tcctatgaaa tttcctcaaa
    80941 attgtgattg tatgaatcaa aaaagtagca atcaatttgt atgtgaatat gggataacta
    81001 tatataatat accaatactg tacaaaagca atagcatagg tgttataaga ggagggtatg
    81061 tactattatc tgatttgaat ttagatacag agcataataa tttatatgat attccagagg
    81121 gagctgctag aagtataaaa cgcctcttga aacaaatatc taaaaatata ataaattttt
    81181 gctcttttaa tgatataaga aaggatttac aagaaaaaga gaagactata gcaagaacat
    81241 atcattatgg agagcaactt gaaatgaatt taaaagtagc tcaagatatg gttactaatt
    81301 taagaataaa tcatcacttt ttatttaata cattaaattc aatggcaagc atagcactag
    81361 atgatggtag ttatgatttg tatagtgcaa taatagattt atcaagaatg tttagatata
    81421 caatgaggtc tgatttaaga tttgtagaat tagaatctga aatattatac attaaaaatt
    81481 atttgaattt acagaaatta cgttatggag atgcacttaa agtaaaatat cttattccag
    81541 agaagttata taacctttct gtgcctttta actttattca acctattgta gagaatgcat
    81601 ttacacatgg atttagagat attgacacag aaaagcgaat tgaaataatt gcaagattag
    81661 attctcagta tgctattata gagattcata ataatggaac tatcttagat ggaaataata
    81721 tagacaaaat aaaggcaggt attcgtagta ataatggtca tggtttgtct ttaatatata
    81781 caaaattcac atctgcttat ggaaataatt ttgatatgga tataaagtcg agtgataatg
    81841 aaggaactta cataatgatt aaaattccta ttgaaaatta taaggagtat gtatagaact
    81901 tgtgaaaaat taataagttt tataaatgtt aagaaatata ttagatgttt ttatgttgtt
    81961 ttatatatct tactttttta tattattaag ctaaaaggga ggtattctca tgagaagagc
    82021 actaataata gatgatgaag aagctgttct aaaaataata acacatttta tagaaatgaa
    82081 agatatgcca ataaaaattg ttggtaaagc aacatctggt gatgaagcag tagataaaat
    82141 cattggttta gagccagaca tagtttttat agatattcaa atgccaatat acaacgggtt
    82201 agaagtaata gaaaaaacat ctcatttatc taaaaaaata aactttgtag taataactgc
    82261 atttaactat tttgaatatg ctcaaaaagc tttgagatta aatgtaaaag atatactcct
    82321 taaaccattg gatatgaaag aatttactaa gtctgtagaa aaaattattg gatatcaata
    82381 taccaataat gatttattaa atgagatact ggaatatata aatctaaact atagcaatga
    82441 cttaaagtta aatgattgtg ctagattatt tttaaccaat ccatctaata tatctaggat
    82501 ttttaaaagt aatttaaata caacttttat atcatatcta aatcatataa gaatagaaaa
    82561 atctattgag ttacttgaaa atactacaaa aagtataaat gagattgcag aaatagttgg
    82621 atataatagc ctaaataact tttataaaaa ctttaagata gaaaaaggaa tgactccaaa
    82681 agtttataaa ctgaacacta aaaattgaaa ttgataaata aaaagtcaat atagataatt
    82741 gtttgagtgt gtctgaataa gtatacttta attatgataa aaattaaagc gctttaaaca
    82801 tatttaataa tattaaacct tataaaagct gtacctattt atataaaaaa atatatataa
    82861 ataaaaagaa aaagatatac aaaataaaaa aagtgaatta aatactgttt caaaaagaca
    82921 gaattttcat ttaatttact gagagtttat atttcttaaa ggaaggtaac tttataatgg
    82981 aggtaattta atatgtcaga caaagtgaat atgaaaaata ttatctgtat tgcaggcgct
    83041 tatctagcaa cagcaattgg ttctgggttt gctactggtc aagaaattct acaatttttt
    83101 gcctcacaag ggtatatggg tttaattgga gcattaatat caatggtatt gttttcattt
    83161 cttggtgctg aagttataac tagaggtaga gaattaaaac ttaaagaacc aacaagaatt
    83221 tacacttttt attgtgggaa gattttagga aaattttacg aatggtttag tccaatattt
    83281 ttatttggga ttcttatagt aatgatttca ggtgcaggct caactttaac tgaatactat
    83341 ggtataaatc cttacttagg aagagtattg atggctgttg ctactttaat tacagtgtct
    83401 atgggtttag aaaaattagt aagcatactt ggacgtattg gaccagtgat agcagtattt
    83461 acaattgctg ttggattaat aagcttgttt aagcattttg atggtttatc tatggttgga
    83521 gaagctatga aaaatattga agttacaaaa gcatcagaaa gtccaatcgt ttctggtgta
    83581 atatataata cattaaatat tattgtaatg attgtttttt taacaggaat gggagcttct
    83641 attgaaagta aaaaggatgc tttttgggga ggtttattag gtggaatagt gtttatgact
    83701 gctggtatag ttatgtattt ggctatgatt tcagatatag gaaatttgta tatgaaagaa
    83761 ataccatcac tttatttagc agataatata tctcctattg taggtgtatg tttttcagtt
    83821 gcattaatat taggtatcta cacaacagct gttccacttt tatggtctgt aaccaataga
    83881 attgttgaag atgaacatcc taaatttaaa ttggtaacaa cagtaatagc tattttggct
    83941 tgtataggag gttttttacc gtttggtaaa ctagtaaaca ttttatatcc atatacaggt
    84001 tatatggggc ttttaatttt agtctgtatg gtgtataaag caattacatg tagaaaaaaa
    84061 tcaacacaaa gtcaggaata gagccactta ttaatagttt ttatttaaag aaatttttta
    84121 tataatccga atttaaaaag tca
//
LOCUS       DAYATN010000003        77339 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.003, whole genome shotgun sequence.
ACCESSION   DAYATN010000003 DAYATN010000000
VERSION     DAYATN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 77339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.003"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(121..894)
                     /locus_tag="ACGI8A_000158"
     CDS             complement(121..894)
                     /locus_tag="ACGI8A_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HFH0178174.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1301)
                     /locus_tag="ACGI8A_000159"
     CDS             complement(894..1301)
                     /locus_tag="ACGI8A_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0178175.1"
                     /translation="MACETIKMEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVA
                     VSEACTNAIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLG
                     LFIIKTLMDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="ACGI8A_000160"
     CDS             complement(1305..1622)
                     /locus_tag="ACGI8A_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HFH0178176.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="ACGI8A_000161"
     CDS             complement(1634..1894)
                     /locus_tag="ACGI8A_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFH0178177.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="ACGI8A_000162"
     CDS             complement(1894..2190)
                     /locus_tag="ACGI8A_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFH0178178.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="ACGI8A_000163"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="ACGI8A_000163"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFH0178179.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="ACGI8A_000164"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="ACGI8A_000164"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFH0178180.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="ACGI8A_000165"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="ACGI8A_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFH0178181.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="ACGI8A_000166"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="ACGI8A_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFH0178182.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /gene="dnaN"
                     /locus_tag="ACGI8A_000167"
     CDS             complement(8083..9189)
                     /gene="dnaN"
                     /locus_tag="ACGI8A_000167"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFH0178183.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10750)
                     /gene="dnaA"
                     /locus_tag="ACGI8A_000168"
     CDS             complement(9431..10750)
                     /gene="dnaA"
                     /locus_tag="ACGI8A_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFH0178184.1"
                     /translation="MDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILLA
                     PSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYPN
                     LNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHHI
                     VSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERTQ
                     EEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIAI
                     LRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEAL
                     KDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTDL
                     SLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="ACGI8A_000169"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="ACGI8A_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFH0178185.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="ACGI8A_000170"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="ACGI8A_000170"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFH0178186.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="ACGI8A_000171"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="ACGI8A_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFH0178187.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="ACGI8A_000172"
     CDS             12283..12990
                     /locus_tag="ACGI8A_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFH0178188.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /gene="jag"
                     /locus_tag="ACGI8A_000173"
     CDS             12959..13588
                     /gene="jag"
                     /locus_tag="ACGI8A_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFH0178189.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="ACGI8A_000174"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="ACGI8A_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFH0178190.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15174..17069
                     /gene="mnmG"
                     /locus_tag="ACGI8A_000175"
     CDS             15174..17069
                     /gene="mnmG"
                     /locus_tag="ACGI8A_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFH0178191.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="ACGI8A_000176"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="ACGI8A_000176"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFH0178192.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKDEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="ACGI8A_000177"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="ACGI8A_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HFH0178193.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18864..19637
                     /locus_tag="ACGI8A_000178"
     CDS             18864..19637
                     /locus_tag="ACGI8A_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0178194.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19639..20502
                     /locus_tag="ACGI8A_000179"
     CDS             19639..20502
                     /locus_tag="ACGI8A_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFH0178195.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20535..21677
                     /locus_tag="ACGI8A_000180"
     CDS             20535..21677
                     /locus_tag="ACGI8A_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFH0178196.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21811..22386)
                     /locus_tag="ACGI8A_000181"
     CDS             complement(21811..22386)
                     /locus_tag="ACGI8A_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HFH0178197.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22646..23938
                     /gene="rstA"
                     /locus_tag="ACGI8A_000182"
     CDS             22646..23938
                     /gene="rstA"
                     /locus_tag="ACGI8A_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin production/motility transcriptional
                     repressor RstA"
                     /protein_id="HFH0178198.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24043..24645
                     /gene="yedF"
                     /locus_tag="ACGI8A_000183"
     CDS             24043..24645
                     /gene="yedF"
                     /locus_tag="ACGI8A_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFH0178199.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24718..24981
                     /locus_tag="ACGI8A_000184"
     CDS             24718..24981
                     /locus_tag="ACGI8A_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFH0178200.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24996..25181
                     /locus_tag="ACGI8A_000185"
     CDS             24996..25181
                     /locus_tag="ACGI8A_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFH0178201.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25284..26480
                     /locus_tag="ACGI8A_000186"
     CDS             25284..26480
                     /locus_tag="ACGI8A_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFH0178202.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26694..26972
                     /gene="rpsF"
                     /locus_tag="ACGI8A_000187"
     CDS             26694..26972
                     /gene="rpsF"
                     /locus_tag="ACGI8A_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFH0178203.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27009..27443
                     /locus_tag="ACGI8A_000188"
     CDS             27009..27443
                     /locus_tag="ACGI8A_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFH0178204.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27462..27689
                     /gene="rpsR"
                     /locus_tag="ACGI8A_000189"
     CDS             27462..27689
                     /gene="rpsR"
                     /locus_tag="ACGI8A_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFH0178205.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27832..28152
                     /locus_tag="ACGI8A_000190"
     CDS             27832..28152
                     /locus_tag="ACGI8A_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFH0178206.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28160..29116
                     /locus_tag="ACGI8A_000191"
     CDS             28160..29116
                     /locus_tag="ACGI8A_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HFH0178207.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29127..31121
                     /locus_tag="ACGI8A_000192"
     CDS             29127..31121
                     /locus_tag="ACGI8A_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFH0178208.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31122..31571
                     /gene="rplI"
                     /locus_tag="ACGI8A_000193"
     CDS             31122..31571
                     /gene="rplI"
                     /locus_tag="ACGI8A_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFH0178209.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31590..32918
                     /gene="dnaB"
                     /locus_tag="ACGI8A_000194"
     CDS             31590..32918
                     /gene="dnaB"
                     /locus_tag="ACGI8A_000194"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFH0178210.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33123..33431
                     /locus_tag="ACGI8A_000195"
     CDS             33123..33431
                     /locus_tag="ACGI8A_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178211.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33513..34802
                     /locus_tag="ACGI8A_000196"
     CDS             33513..34802
                     /locus_tag="ACGI8A_000196"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFH0178212.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35128..35203
                     /locus_tag="ACGI8A_000197"
     tRNA            35128..35203
                     /locus_tag="ACGI8A_000197"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35161..35163,aa:Lys,seq:ttt)
     gene            35210..35284
                     /locus_tag="ACGI8A_000198"
     tRNA            35210..35284
                     /locus_tag="ACGI8A_000198"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35243..35245,aa:Glu,seq:ttc)
     gene            35289..35364
                     /locus_tag="ACGI8A_000199"
     tRNA            35289..35364
                     /locus_tag="ACGI8A_000199"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35322..35324,aa:Val,seq:tac)
     gene            35398..35472
                     /locus_tag="ACGI8A_000200"
     tRNA            35398..35472
                     /locus_tag="ACGI8A_000200"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35430..35432,aa:Thr,seq:tgt)
     gene            complement(35855..36847)
                     /locus_tag="ACGI8A_000201"
     CDS             complement(35855..36847)
                     /locus_tag="ACGI8A_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0178213.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36856..37914)
                     /locus_tag="ACGI8A_000202"
     CDS             complement(36856..37914)
                     /locus_tag="ACGI8A_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFH0178214.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38039..39502
                     /locus_tag="ACGI8A_000203"
     CDS             38039..39502
                     /locus_tag="ACGI8A_000203"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HFH0178215.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39604..40401
                     /locus_tag="ACGI8A_000204"
     CDS             39604..40401
                     /locus_tag="ACGI8A_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0178216.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40414..42516
                     /locus_tag="ACGI8A_000205"
     CDS             40414..42516
                     /locus_tag="ACGI8A_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0178217.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42584..43063
                     /gene="rlmH"
                     /locus_tag="ACGI8A_000206"
     CDS             42584..43063
                     /gene="rlmH"
                     /locus_tag="ACGI8A_000206"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFH0178218.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43212..43523
                     /locus_tag="ACGI8A_000207"
     CDS             43212..43523
                     /locus_tag="ACGI8A_000207"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0178219.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43571..43888
                     /locus_tag="ACGI8A_000208"
     CDS             43571..43888
                     /locus_tag="ACGI8A_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFH0178220.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44101..44802
                     /locus_tag="ACGI8A_000209"
     CDS             44101..44802
                     /locus_tag="ACGI8A_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0178221.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45026..46327
                     /locus_tag="ACGI8A_000210"
     CDS             45026..46327
                     /locus_tag="ACGI8A_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0178222.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46464..47558
                     /locus_tag="ACGI8A_000211"
     CDS             46464..47558
                     /locus_tag="ACGI8A_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFH0178223.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKLILEGE"
     gene            47560..48693
                     /locus_tag="ACGI8A_000212"
     CDS             47560..48693
                     /locus_tag="ACGI8A_000212"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFH0178224.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELRRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48711..49820
                     /locus_tag="ACGI8A_000213"
     CDS             48711..49820
                     /locus_tag="ACGI8A_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFH0178225.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49885..50625
                     /locus_tag="ACGI8A_000214"
     CDS             49885..50625
                     /locus_tag="ACGI8A_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFH0178226.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGILSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50630..51622
                     /locus_tag="ACGI8A_000215"
     CDS             50630..51622
                     /locus_tag="ACGI8A_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFH0178227.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYITTGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51810..52691)
                     /locus_tag="ACGI8A_000216"
     CDS             complement(51810..52691)
                     /locus_tag="ACGI8A_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0178228.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52964..54352
                     /locus_tag="ACGI8A_000217"
     CDS             52964..54352
                     /locus_tag="ACGI8A_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HFH0178229.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYANGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54380..55333
                     /locus_tag="ACGI8A_000218"
     CDS             54380..55333
                     /locus_tag="ACGI8A_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0178230.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            55393..55617
                     /locus_tag="ACGI8A_000219"
     CDS             55393..55617
                     /locus_tag="ACGI8A_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178231.1"
                     /translation="MNEKSSFKQIEIILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55610..56122
                     /locus_tag="ACGI8A_000220"
     CDS             55610..56122
                     /locus_tag="ACGI8A_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFH0178232.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            56154..57008
                     /locus_tag="ACGI8A_000221"
     CDS             56154..57008
                     /locus_tag="ACGI8A_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HFH0178233.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57542..58336
                     /locus_tag="ACGI8A_000222"
     CDS             57542..58336
                     /locus_tag="ACGI8A_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0178234.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            58440..59246
                     /locus_tag="ACGI8A_000223"
     CDS             58440..59246
                     /locus_tag="ACGI8A_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFH0178235.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            59308..59829
                     /locus_tag="ACGI8A_000224"
     CDS             59308..59829
                     /locus_tag="ACGI8A_000224"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFH0178236.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            60244..62331
                     /locus_tag="ACGI8A_000225"
     CDS             60244..62331
                     /locus_tag="ACGI8A_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0178237.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMDDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            62346..62633
                     /locus_tag="ACGI8A_000226"
     CDS             62346..62633
                     /locus_tag="ACGI8A_000226"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0178238.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            62660..63931
                     /locus_tag="ACGI8A_000227"
     CDS             62660..63931
                     /locus_tag="ACGI8A_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFH0178239.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            63989..64588
                     /locus_tag="ACGI8A_000228"
     CDS             63989..64588
                     /locus_tag="ACGI8A_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFH0178240.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQEYLKWHPAGSLGKSIREGI"
     gene            64593..65498
                     /gene="dapA"
                     /locus_tag="ACGI8A_000229"
     CDS             64593..65498
                     /gene="dapA"
                     /locus_tag="ACGI8A_000229"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFH0178241.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKSQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            65604..66236
                     /locus_tag="ACGI8A_000230"
     CDS             65604..66236
                     /locus_tag="ACGI8A_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HFH0178242.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNANKIREIIKNVRKI"
     gene            66713..69220
                     /locus_tag="ACGI8A_000231"
     CDS             66713..69220
                     /locus_tag="ACGI8A_000231"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0178243.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            69562..71724
                     /locus_tag="ACGI8A_000232"
     CDS             69562..71724
                     /locus_tag="ACGI8A_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFH0178244.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            71729..72346
                     /locus_tag="ACGI8A_000233"
     CDS             71729..72346
                     /locus_tag="ACGI8A_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178245.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLDLSEQADRVIKL"
     gene            72535..72879
                     /locus_tag="ACGI8A_000234"
     CDS             72535..72879
                     /locus_tag="ACGI8A_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFH0178246.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            73449..74315
                     /locus_tag="ACGI8A_000235"
     CDS             73449..74315
                     /locus_tag="ACGI8A_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFH0178247.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPINK
                     ESGKPCINLQPDFKCSVHNSLIKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESMMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            74428..74832
                     /locus_tag="ACGI8A_000236"
     CDS             74428..74832
                     /locus_tag="ACGI8A_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HFH0178248.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            74996..75445
                     /locus_tag="ACGI8A_000237"
     CDS             74996..75445
                     /locus_tag="ACGI8A_000237"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0178249.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(75601..75978)
                     /locus_tag="ACGI8A_000238"
     CDS             complement(75601..75978)
                     /locus_tag="ACGI8A_000238"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0178250.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            76249..77157
                     /locus_tag="ACGI8A_000239"
     CDS             76249..77157
                     /locus_tag="ACGI8A_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HFH0178251.1"
                     /translation="MNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKCMNV
                     PIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSINAF
                     IKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINRLPV
                     LIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDIETL
                     NTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKYYLE
                     KTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT        29598 a         9144 c        12883 g        25714 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctattgcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctagtaagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcat taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag taataggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctatta ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttactag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga attttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 aggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga cattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaacttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggt ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gccttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggtg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagagaa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaaaggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ctagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgactgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaagatgaag actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataagcc ttgtaatata
    18781 aattgattta tagacattat ttatacttaa aaaacaccta ttcaacattt aaaattaatt
    18841 taattcttaa aggggggaac atcatgggca aagttatagc tgtattcaat caaaagggtg
    18901 gagtcgggaa aactacaaca aatgtaaatt taagtgctag cttaggaact ttgggaaaga
    18961 aaatattagt attagattta gacccacaag gaaatacaac tagtggatat ggtattaata
    19021 aaaacgaagt agaaaataca atctatgaaa ttatgttaga tgggttacat attaaagaag
    19081 caataataag tactgaattt gagaatgtag atgtagttcc atcagctact gaactatcag
    19141 gtgcagagat agagcttaca tctaaaacta atagagagta tattttaaaa aattccataa
    19201 aagcggtaat agatgaatat gattatatat ttttagattg tccaccctca ctggggatgc
    19261 ttacaataaa ttgtttaact gctgtagata gtgttttaat tccaatacaa tgtgaatatt
    19321 atgctttaga aggtgttagt caattaatgg agactataaa attagtaaaa tcaagattaa
    19381 atgctgatat agaaattcaa ggtgttgttt taagtatgtt tgatggaaga gctaatttat
    19441 caatacaagt agttgaagaa gttaagaaat actttaaagg tagtgtatat acaactttaa
    19501 ttccaagaaa tgtaagactt gcagaagctc caagtcatgg aaaacctgta atttattatg
    19561 ataaaagatg tagagggtct gtagcttatt tggagctagc agaagaattt atagatttag
    19621 aagaggagga atggtagtat ggagaataag tctaaaagaa gtaatagatt aggtagaggt
    19681 cttagtgcat tgataccaga aattaaagga gaaacttcag aaaaagaaat agtcaacata
    19741 gatatagaca aaatatatcc aaacgaagtt caacctagaa agcaatttga tgaagaaaag
    19801 ataaaagtat tgtcggattc tataaaaaat tatggtgttt tacagccaat agtggttaaa
    19861 atggatgaaa ataataaata tatgataata gctggagaaa gaagatttag agcatcaaaa
    19921 cttgcaaata aaaatcaaat acctgctata attaaagata tagatatgaa agatataatg
    19981 gagatagcct taattgaaaa cttacagagg gaagatttaa attcaataga agaggctcta
    20041 gcatataaga gtttaataga gcattacaat gtaactcagg aagaaatatc agaagctgtt
    20101 gggaagagta gacctcatat aactaataca ctaagacttt taaatcttgg acaagatgtt
    20161 atagaaatga tagacagtgg tagaatcaca gcaggccatg gtaaggcttt attgagaatt
    20221 gcagataaag atttgcaact tcaaatagcg aaaaaaatag aagaagaaga actttctgta
    20281 agagaagtcg aaaatatagc taaaaaaata tctgaaaata aacaagaaga acctaaaaaa
    20341 tctaaaccta aggatgtatt tattttagat gttgaagata aacttagaaa tatatttgga
    20401 acaaaggtga atatttctaa aggaaagaaa aaaggtaaaa tagagataga atactataat
    20461 gatgatgatt taaatagtat tgtatcaatg cttcttgaat agaacaaaaa tataaaacag
    20521 agaggaagat tgttatgatt tatttagata atgctgcaac tacatatcca aaaccagaaa
    20581 gagtgtataa tgctgtttta gattgtatga aaaattattg tgcaaatcca ggtagagcag
    20641 gccataaact tgctatgaga gctgctaggg agatatatga tacaagagag aatatagcta
    20701 agttgttcaa tgtaagtaat cctatgaata tagtatttac atctaatgca acagattctt
    20761 taaatttagc gataaaaggt gtattacaag aaggagacca tgtaataaca acgtctatgg
    20821 agcataattc agtaataaga cctataaaag cattggaaaa aaggggtatt gaaaatactg
    20881 tagtaaaatg cgattatgag ggatttttag attatgaaga tttagaaaaa tcaataaaga
    20941 gtaatactaa actgattgta acaacacatg cgtctaatgt atgtggaact ttaatagata
    21001 taaaaaaagt tggagaaata gctaaaaaac ataatatact atttttagta gatgcatcac
    21061 aaacagcagg tgtttatgat atagacgtaa atgaatgtaa tatagatatg ttggcaatgc
    21121 caggtcataa atgcttattt ggacctcaag gaacaggtat attatatgta agagaagggt
    21181 tgaacttaaa tatattaaaa gaaggtggaa caggtagtaa atcagaagaa atagttcaac
    21241 cagagttatt cccagataaa tatgaatcag gaactcataa tactccaggt atagcaggtc
    21301 ttaaccaagg tatactattt atatttgaaa gaggaatcaa caatataaga caacatgaag
    21361 aagaattatg tcaatatatg atagacaaat tagaggaagt accagatata aagatatatg
    21421 gaccaaagga cagtaaaaaa agagcatcag taatagctct aaatatagga gatatggatt
    21481 ctggagaggt tactttctta ttagatagtg attataatat agcgactaga tcaggaatac
    21541 attgttcacc actagcacat accactttag gtactttaaa acaaggtgca gtaaggttta
    21601 gtataggata tttcaacaca aaagatgaga tagataaagc agtggaagct ttgaaaaaaa
    21661 tttcaaaaaa taaatagcat gagatttaat aatatagaaa taaaaaaagg ggatatttca
    21721 aaataaaaat ttgaatcatc tccttttttg tacaaaaaag aaaaggggtt cctaagaacc
    21781 tctaatctat ttgataatag tttcaagaaa ttactttaag taatctcctt cttcaagaac
    21841 tatatgacca ctttcgtaat tagaaccatc aacagtaatt tttatttgtt caacattcat
    21901 attttttatg taagtacgag caactgaatc aagcattaaa gtttctgcat cactacctaa
    21961 gtttgacttt gtaaagtcag agctaacatc taatatacca gtttttacac catcaatgtc
    22021 ttttgtagta aatgaattta attttgcaga ttctggaaca actttaagtt tttgaagttc
    22081 ttcaaataaa gtgttctcat caattttatt taaatcaact ttattttcaa ttaaatctgt
    22141 cttatctaca tcaaaagagt aaatagtagc tgattgagtt ttagtttctt cttttggagt
    22201 aggtttagct ttgttattag gaacctcttt ttcagtattg ttttcttctt ttggttgctc
    22261 aacatttgta ttgctcttag tttctttttt agatgaatca cttccatttt gagcattagt
    22321 acaaccaact gcgaatatag atatagataa tatacttaac actaacatta ttttcttatt
    22381 tttcataaaa atttctcctc tcttaaaaac tattcttata tataattata gtcgttatga
    22441 gcaaatatat gaatacaaaa tagtaacaaa tttaaaaaat gatttattta aattaataaa
    22501 actcaaaata aactaaattt tagtcatttt tatgggaaat catttaatgt acagtgaaaa
    22561 tattgtaaaa taaaagtagt gtttttaaaa taaatataag ttataattat attataaaaa
    22621 aatgtcttca aaaggtggga atagtatgga aattttgagc ttaggagaaa agattaaaaa
    22681 attaagaaaa gaaaaaaaca tgacattaaa agaattagct ggagatagga taacagcagc
    22741 acaaataagc catatagaga gggataaatc tcatactagt tatgaactgt tggaatatct
    22801 aggcgataag ctagatgtaa gtgtagaata cttattagag accaaggaaa tgcaatcaaa
    22861 aaagataact gataatttaa tactacagag cgaaatttat ataaaagaaa atgaattaaa
    22921 caaggctgag gaccaaataa aagaagtttt agatatatgt aagagatacg atttaatgga
    22981 gaactatggc agatgtaatt ttttaatggc taatattaat ttgaaaaaat ctaattataa
    23041 tgatgctata gaaaactttg aaaaggcatt gtacttcttt ataaaaaata gtgatagtga
    23101 aaatatactt aagtgttatt taaacatagg gaaaatatat atgcaggaag acttttataa
    23161 aggtgctata agccacttta attttgcaga ggaaattttg tcagagaata aatttgagga
    23221 tgttaacata cacaaggaac tatatagtaa gatggcatat tgttacataa aactagataa
    23281 tccaaatatg tcattgaagt acatagaaaa gataaatgaa atagataata gaaatgatag
    23341 aaaagaagat gtcgatattt tggtgttaaa agcaaataat atgcttgaga ttggaaaata
    23401 tgaagaatca agagattact ttaaaaaagc actaaaaata cttgagagtg aggataataa
    23461 aactggatta gcaaatgtgt atttaacaat ttgtgatgtt tacagaaaaa taggtgaaac
    23521 agatagagta cttgaatatt cacaaaaggt atatgacata aagaaaaatg atgaagatga
    23581 atacatgatg tcaggattat ttaagataat agaagcatat atagataaag aagattatga
    23641 tttagctaga aaatattgta agatagcctt agcttcatca ataaaaaata aaaataaatt
    23701 caatgaatat aaagcattga gattctatgc taatatgtat aaaaatcaga atgaagtaac
    23761 tcttgcaatt gaatatctga ttaaatgcat aaaaatagta tcagatttag gcaacaatag
    23821 aatattggcc aatttatata tagacttagg tcaattatat tcaaatatat caaaagaaaa
    23881 agagttagaa tattatcaaa aaggtgtatt tatgtacaaa aatttagaaa taatgtaggg
    23941 taaataaacc tataagggta tagaaacaat ctataacaag caagataata tctatgataa
    24001 aatataaatt gtatgaatat ttttaaggag atgaatcaga caatgagtat aaaaatagat
    24061 gcgagagggc ttgcttgtcc taaaccagtt ataaatacaa aaagagaatt ggataaccta
    24121 gaagaaggtg ttgtagttac aatagtcgat aatgaaacag ctaaggagaa tatattaaaa
    24181 cttgctaaat ctttaagttg tgaagcaaat attgttgacg aaaaagagaa ttttatatct
    24241 atagaaatta gaaaaggtaa aaatatacta gataaacaaa atgttgtaga taataaagaa
    24301 agtgaattag acaatacatg tatatttata tcttcagata agatgggact tggaaatgac
    24361 gaattaggaa aagtgttaat aaaaggattt atatatacat taacagaatc taaaccttat
    24421 cctaagtatg tacttttagt taatggagga gtaaaattaa gtgctgaaaa tgaagaaacc
    24481 atagaaaatc taaaaatact tgagaatatg ggtgtagaag ttttatcttg tggtacttgt
    24541 ttagattatt ataatttaaa agacaaatta caagtaggta cagttacaaa tatgtatact
    24601 atagttgaga cattaaagaa tgcatcaaat accatttcta tataatgtat gtttgtttaa
    24661 taatataaaa ttaataataa agattaactc aaatagatag tcagaaagga ttaagtaatg
    24721 aatcaaatgt atatagtgtc atttaattcg acacatcatg cgataagatc agaaaagtta
    24781 tttggtgaaa atagtctaaa agtaatggct cttccaacac caagagaaat aacagcaagt
    24841 tgtggaatat caattaaatt ttcttttgaa gatatggaaa agataaaaac tattttagtt
    24901 gaaaataatg tagatataaa aggaatttat tgtataagta aattagaaaa tggctcaaaa
    24961 gaagtagaga agttagatta ggaggctaat tatttatgcc aatggattta aaaataggtg
    25021 atattgttga attaaaaaag caacatgcat gtgggtgtaa agagtttgaa atcataagaa
    25081 cgggaatgga tataaagata aaatgtacta aatgttcaag gctaataatg cttgatagag
    25141 agaccttaga aaagagagta aaaaagctaa taaaaaagtg ataaaaattt aacacttaaa
    25201 aaatgtcaaa taccgtatat taatatccaa aattttggta taataagaat tgaagaaaac
    25261 attttcatgg gggtgcttta attatggcag ttaaatatgc aaaaagaatg caaggattac
    25321 aaggatcaga aatacgtgag cttttaaaac ttactcaaca accacaaata atatcttttg
    25381 ctggtggaat gccggctcca gagttatttc cagttgagga aatgaaaaaa gtatcagttg
    25441 cagtgcttga agaaaatgga agatcagcta tgcaatatac tacaacagaa ggatatgaac
    25501 cattaagaga aaaaatagca gctagaatga atgataaaaa caaaactaat gtaaacaaag
    25561 atgatatatt agttacaagt ggttctcaac aaggtctaga ttttgcagga aaagtattta
    25621 ttgatgaggg tgatgtaatt ttatgtgaaa gtccatctta cataggagct ataaacgcat
    25681 ttaaatctta tcaacctaaa ttcatagatg ttccaacaga ttctgatgga atgataatgg
    25741 aagaattaga aaagatacta gaaacaactg atagaataaa aatgatttat gtaatacctg
    25801 acttccaaaa tccaacagga agaacttggc cacttgaaag acgtaaaaaa ttcatggaaa
    25861 tagtaaataa atttgaaata ccagtaatag aagataatcc atatggagat ttaagatttg
    25921 aaggagagac tttaccatct ttaaaatcaa tggatacaaa aggattagta atattcttag
    25981 gaactttctc taaaatattc tgcccaggat acagattagg atggacttgt gcttctccag
    26041 aaattttatc taagtttaac tttgcaaaac aaggtgcaga cttacaagca tcaactatat
    26101 ctcaaatgga agtaagcaaa tttatggata tgtatgacct agatgcacat gttgacaaaa
    26161 taaaagcagt ttatgttaaa cgtagagatg taatgcttaa gactatggaa gaagagttcc
    26221 cagaaggatt agtatttact catccagaag gtggattatt tacatgggta gagcttccaa
    26281 gtaatttaaa tgctaaagaa ttaatgccaa aatgtttaga taagaatgtt gcttatgttg
    26341 ctggaggagg attcttccct aatggtggta gagaaaatac tttcagactt aactattcaa
    26401 atatgccaga agaaaaaata atagaaggta taaaaaatat agcagctgta ttaaaagaag
    26461 ctatgggtgt agaagcttaa ttaaaaataa taacttatat ataatacaag ggatggttgt
    26521 tgccatccct tgtttgtttg aaaagtatat aattcacctt atactaaact ttgtccaaaa
    26581 tttaaattga cagaaataag aataaatgat aaaatgtgta ggtatgagat tttagtctca
    26641 tttctctgct ctaagagtgt agagccgtta agtccaaagg gaggtgaatt atagtgagaa
    26701 attatgaatt agtttatgtg gtaaagccaa actctgatga agaagtaaga gaggctatac
    26761 taaacaaagt taaggaagtt gttgcaactg acggagaaat agttaaagtt gatacttggg
    26821 gaactaaaaa attagcttat ccaatagcta agtttacaga aggtttctat gtattagtta
    26881 acttcaaatc agcagtagac gttcctaaag agatagacag aaacttaaag ataaatgaaa
    26941 acgtaataag acacatgata gttgttgctt aataactggt tttattataa aaaataggtg
    27001 gtgtttttat gaatcaagtt gtattagtgg gaaggttgac tagagaccct gaattaagat
    27061 acatcccagg tacaggaaca ccagtggcat cctttacaat agccattgat agagactatg
    27121 taaaaaaaga tggatctaaa gaaactgatt ttatacctgt tgaagtaatg ggtaaatctg
    27181 ctgaattttg tgctaactac ataactaaag gtagattagt agcacttcaa ggatctataa
    27241 gagtagacaa ttatcagaca caatcaggtg aaaagagaac ttttacaaaa gtaagtacta
    27301 gatcagtaca agctttggat agtaagaata aatcggaaaa ttcatataga gagagtgctc
    27361 cagcttttga gccaagcttt gaacctcaag gtttagaccc acaaggtttc caagctatag
    27421 atgatgacga tataccattt taaaaaggag ggaaaaaagt catgatgaat aaaaaaagac
    27481 gtaagaaaaa aagagtttgt caattttgtg ctgacaaaaa cgctaaaata gattacaaaa
    27541 gtactcaaag attacaaaag tatataactg aaagaggaaa gatattacca agaagaatat
    27601 ctggaacttg tgctaagcat caaagagaat taacagttgc tataaaaaga gcgagaaata
    27661 tagcactttt accatataca ttagactaaa aaaatgagcc ttgtgctcat tttttattat
    27721 atgtatttat atatctaaat gaggcgagct ataaagctcg tttttttgtt tatatactta
    27781 aatatattat tatttatgat ataatattat atattaagga gggttgtaaa cgtgaaaata
    27841 gataaaggtt ctgagataac gagaaacata aagataatag aatggatgaa aacagaaata
    27901 ttaatgagtg taagtgattt gtttaattta ttgtttaaag gggtaaaacc attagatgaa
    27961 gcaattcaag atactttagc aaatataata atgataacat atctcttagc aaagagatta
    28021 ggaattagtt ttagagatgt ggattataaa gtcaaggaaa aaataaaaat tggtatagat
    28081 gaggaccata gcgttgaaag atggtatgga gatttatcta atttaaaaaa acatatggat
    28141 aatagggagt gatattaaat tgaataataa aattagacta tctaaggcaa tgtctataat
    28201 agttttagct ataataatcg ctttagtaat agcctatatt ccaatgctag gaatatttag
    28261 tatgttagca gctgttccat atgtaattat aggtgctatt actgatagaa agtattctct
    28321 tatatctatt ttggttacat tttgtgtact gattctattt gcagacattt cttatgcatt
    28381 aaatatatgt ataatgtatt ctttaccagg tatagtaata ggaaagatgc taaagagaag
    28441 ctttgaacaa gaggacagta ataaattcga accgatatat ggtggaacca taatatttgt
    28501 actttcaatg cttgtatatt tttttgtatt aaagacattt ttaaatgtga atctattaga
    28561 tgaagtttct aagatgatat cagaaattgt aaatattcaa aaaaatagtt tttctgcaac
    28621 tgaacttaaa gtgtttgata aaatgaagcc agatgagatt gtaagttatt ttacaaatat
    28681 gctaccaatg atgttatttt tacaagggtt gttatcagcg tttgtcacat attatttgag
    28741 tgtatttttt ataaaaagga ttacaaagat gaatataaga tttcctaagt ttgctgattt
    28801 ttatttaccg ggaaatgcta tactcacaac tttcttactt tatttattag tcttatttat
    28861 tcaaattatt ggttcaaaat tatatacaga attaataatg actaatttac agctagtttt
    28921 taatttaatg tttgtaattc aaggtatagc agtttgcatt tatttcttaa agaaatggat
    28981 aagacaggga ccaaataaaa ttatgttttt ttcagggata attttatgtt tatttggatt
    29041 tatgggaata tcttttgttg gtatggtaga tagtataatt gactttagaa aggtaagaag
    29101 ctataaatcc acttaggagg atagaaatga gtaataaaca aacctttaaa ttaaatatgc
    29161 cagaaataaa tttatatata attgttattg gtatttctag tataattttg ctttattaca
    29221 acttgtatgt agggtgttta tttttctgta tttttgtata tatggttttt cacaattgga
    29281 gaactactaa tattagacgt catgaatgga cagagtatat tcaaaattta tctttagata
    29341 tagatgaaac aactaaaaaa gccataataa atcttccaat accattatgt attttagaat
    29401 ttgatggaaa tatatcctgg tataatggaa agttttatga tatgattggt caaaaagatt
    29461 tacttgataa aaatatagag gatatagtta aaaatcttaa tttaagaaaa gtattaaatg
    29521 aaaataaaga gatgtacaca gaaataaact ataaagaaaa agaatataca ataatttata
    29581 atgttataaa aaatgatcaa gaaaaaaatc ctaaatattt gatgatatta tactggatag
    29641 acaagactga atatttaaaa gtaaaacaaa attatgatga tgaaaaaaat gcaatgatgc
    29701 taatacaagt agatggatat gatgaagtat taaaaagtgc agcagaagat aagagagctt
    29761 taattaatgt tgaagtagag aaaatacttt ctgctttaga attaaattca aatggtgcat
    29821 tgagaagaac ttcaaaagac aaattcttct tggtaatgca taaaaaagag ttaaaaaaat
    29881 tggaagctga gaagttttct attttagata caataagaca tattgattat ggaaataatc
    29941 ttcctgttac tataagtata ggaattggta tagatggaga tacattaaat gagaacctaa
    30001 aacttgctac gggtgctctc gatttagcac ttggtagagg tggtgaccag gctgttgtaa
    30061 aaacaaaaga taaatttgtg ttttatggag gaaaatctaa agctgtagaa aagaaaacca
    30121 aagtaaaatc cagattaata ggtcatgctt taagagaggt tatacaacaa agtgaccaag
    30181 tttatataat gggacataaa tatcctgata tggatgctat gggagcagca gttggtgttt
    30241 atgatatatg taaatcctgt aacaaaactg caaacatagt tttacaatct gtaaatgaat
    30301 ctatagaaat atttataaat aaaataaatg aaaataatta ctataaaaag ttgtttattg
    30361 gaaaagagga agctattgac aactgcacta aaaatacatt agttgtagta gttgatactc
    30421 atagaccaaa ttatactgaa tgtgaagaac tgttaaagct atcagagaag gtagtagtta
    30481 tagaccatca tagaagaggt gtagagttta taaatgatgc agtgctttta ttccatgaga
    30541 tatatgtatc ctcaacgtgt gaaatggtta cagagttagt tcagtatatg gatgaagatg
    30601 taacaataaa taaacttaca gctgaagggc ttttagcagg tataagtcta gatactaaaa
    30661 actttgcatt caaaacaggt gttagaactt ttgaagctgc ttcttacctt agaaaagttg
    30721 gagcagatac tatagaggta aagaaattct tcaattctga tgtaaaagat ttcataataa
    30781 aagctgaaat aattcaaagt actaaaataa ttaataatag aatatgttta gcatattcaa
    30841 gtactgagat agatagtata aatgttataa tagctcaaac tgctgatgag ttattaaata
    30901 tcaaagaagt agaagcttcc tttgtattag gtgaaaaaga tgatactata tttataagtg
    30961 cgaggtcttt aggacaaata aatgtacatg tacttatgga aaaattagga ggcgggggac
    31021 acatagatat agctggagca cagttaaaaa atgtttctct aaaagaagct tataagatgg
    31081 taaataaaat aatagaggag tatttggagg aggaagaata gatgaaagtt atattattaa
    31141 aagacgtaaa aggaactggt aaaaagggag aaatgaaaga agtaagtgat ggatatgcaa
    31201 gaaatttctt atttcctaaa aagatggctg tgcaagcaga tagtgtagca ataaaagaat
    31261 taaatgaaaa gaataaatct aaagaaataa aagcacaaaa agagtatgaa gaagcagttt
    31321 tattaggtaa gcaaatggaa gaaattaata tagaaatata ttcaaaagca ggagaaggtg
    31381 gaagattatt tggttcaata acttctaaag aaattgctga gcaattaaag aaacaaaaag
    31441 acatagatgt agataaaaga aaaatattat tagatgaacc aataagatct ttaggttcaa
    31501 ctttcgtaga aataaaaata catcaaaaag taactactaa aataagagtg gatgtaaaag
    31561 aaaaacagta ataactagag gtgattaaga tggaagatat gacgagaatt cctcctcata
    31621 gtgtagaatc agaacaatca atattgggtt ctatacttct agataaagat gctataataa
    31681 cggttacaga aactataaag cctgatgatt tctataaaga ggctcacaaa ataatatatg
    31741 aatgtatgat aactttaagt aataaaggtg agcctataga cttgataaca ttaacagaag
    31801 aactaagaaa acaagggcat cttaatgata ttggaggaat cagttatatt acaagtcttt
    31861 ctactatagt accgacgact tcaaatgtaa aatactatgc tgatatagta aaagaaaagt
    31921 ctgtacttag aaagcttata aaagcttcta atgaaataat aaatctagga tatagtggag
    31981 caactaagat tgaagatgtt ttagaacaag ctgaaaagag tatatttgat atatcacagg
    32041 agaaaactag tgatgatttt aaatctataa acttagtttt gatggatgcc tatgatatga
    32101 tagaaaaatt atacactaac aagagtgatg tcactggtat aactactggt tttaaagatt
    32161 taaacaaaaa aataaatggt cttcaaagga ctgatttaat attaattgca gctagaccag
    32221 caatgggtaa aactgccttt tctttaaact tggttcaaaa tgcagcctta aaaggtgatg
    32281 cctctgtagc tgtatttagt ttagagatgt ctaaggaaca attagtacaa cgtatgttat
    32341 cttcacaatc tagtgtagaa ttaaaaaaaa taaagactgg aacacttaat gataatgatt
    32401 ggcctagaat tatagatgct atggcagtat tatcagatgc caaaatacac atagatgata
    32461 ctcctggtat aaaaatatca gaactaaggt caaagtgtag aaagttaaaa atagaaaaag
    32521 gactagattt agtgcttatt gattatcttc aacttatgga aggtgaaggt aataatgaaa
    32581 gcagacaaca agagatatct aagatatcaa ggtcactaaa aatattagca aaagaattaa
    32641 actgtcctgt agtagcacta tctcagttat cacgtgcacc agaacaaaga gcagaccata
    32701 gacctatgtt atctgattta agagagtctg gagcaataga acaggatgca gatatagtta
    32761 tgttcttata cagggacgaa tattatcatg ctgattctga gagtaaaaat ataggagaag
    32821 taataatagc aaaaaataga cacggtgaaa ctggttctgt ggaacttgtt tggcttggtg
    32881 aagttcaaag atttggggat aagttgagag acctataatc taacgaatat ccacagaacg
    32941 tcctttcttt ctacaggaaa aatgtggata catgtcacta aatatgtcca aacattgaaa
    33001 tttagctaaa aaactatcca cactatccac agatggcctg ttgataagtg tgaataagta
    33061 gataaagcaa gcttttggct tgctttatct gtgtataaat attaattatt aggaggaata
    33121 atatggaaaa aataagctta aaatatatat atcccaatat aattaaagta cttgatgaaa
    33181 taaacttatt tagagtaatt gacaataatt taagagaatc tattgttgtt tatgctaata
    33241 atgtggataa tcagtatcat ataaacatga ctaatacaaa ctttggaaat ataattaaca
    33301 tatgtaaatt agaaaaactt ttagatgtgg ataaatttat ggaaaaagtt ataaaatatg
    33361 aaaaagaaat aatagagaaa gaagagtttt caaaaataga agaatatatg ttaaatattg
    33421 gagaatacta aaaaagttga aaaaaatacg aatgtttgat agaataagta agtaaagtgt
    33481 tcgagagtat ataaaatata gaggtgaatt acatgaaaac agttgcaatt gttggttctc
    33541 aatggggaga tgaaggtaaa ggtaaagtta tagattatct tgctactcaa gcagatgtag
    33601 tagtaagagg gcaaggtgga aataatgcag gacatacatt agttgtagaa ggtaaaaaat
    33661 acgcattaca tctaattcca tctggagttt taaatccaaa tacagtgaat ataatcggaa
    33721 atggaatagt gtttgaccct aaaggatttt tagaagaatt ggaaatgttt aaaacggata
    33781 atataagtac agaaaatata aaaataagtg atagagcaca tgtcatattc ccatatcata
    33841 aagaactaga tgcattgtca gaagaagcca gaggcgattt aaagataggt acaacaaaaa
    33901 aaggtattgg accttgttat atggataaaa cagagagatc tggtattaga atatgtgact
    33961 taatggataa agataaattt gctataaaat taaaagctca aatagatgct aaaaatgaaa
    34021 tagttaaaaa tatatatgga aaagaagaat tatttgattt tgaaactata tataacgaat
    34081 atctaggata tgcagagcaa ataagaaaat atgtggcaga tacgtctgtt attgtatatg
    34141 atgcagtaag agcaggtaaa aaagtattgt ttgaaggagc acaagggact ttattagatt
    34201 tagatttagg aacttatcca tttgttacat cttctcatcc aacatctggt ggatttgcaa
    34261 ttggtgcagg tatagggcca aatatgataa aagacgttgt tggtatagta aaagcatata
    34321 ctacaagagt tggagaagga ccgtttgtta cagagcaaat aaatgaaact ggagataaga
    34381 taagagaaca aggacatgaa tttggggtaa ctactggtag accaagaaga tgtgggtggt
    34441 ttgatgcagt tatagttaag tatgcagcta gagttaatgg attgactagt atttcattta
    34501 tgttacttga tgtattaact ggatttgata agataaaagt ttgtacgtct tataaaatgg
    34561 gcgataagat aataacggat ttcccagctt cattagatga tttagcaaaa tgtgaacctg
    34621 tatatgaaga attagatgga tggaatgaag atataactca aatagataac tttgatgacc
    34681 ttccagaaaa tgcaaagaaa tatgtagcaa aaatagaaga actagttgga gtaagtgtag
    34741 atatggtttc tgttggtcca aatagagctc aaactataat aagaagaaat atatttgctt
    34801 aatattttat caattaaata atttaaaata gttgaagaag agtatctcaa gataatgttt
    34861 atagttatta gagatgctct tttatatttg acttacattg attttttatt aagttaaagc
    34921 tacttataca tattctaact gaaattaaag ttgaatttat gtagatacat ataggttttt
    34981 taaaaagaat tttgttgaaa ataaatggct aaagatgatt ttaattatat tctaaaaaaa
    35041 gtaaaaaaaa ctattgacta aaaattatat agacgctata atataacttg tgagtgaaag
    35101 aaacgacgaa aaaagtcgaa aaaatgtggt ctattagctc agtcggtaga gcacctgact
    35161 tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca ccaaaatatg gctcgttggt
    35221 caagaggtta agacaccgcc ctttcacggc ggtaacaggg gttcaattcc cctacgagtc
    35281 accagtatgg gactatagct cagctgggag agcacctgcc ttacaagcag ggggtcacag
    35341 gttcgagccc tgttagtccc accaaatttt gaattagaga taaatactag attatatgct
    35401 ggtgtggctc aacggtagag cagctgactt gtaatcagca ggttgtaggt tcgattccta
    35461 tcaccagctc cacaaagtac ataaattcaa attaatataa ttttctatgt tacaggatac
    35521 ttttaagtat ctttttttaa tttcattttt attgtattaa aaatgaaatt aaacatacaa
    35581 caaaattttg aattacttgc aatgtgatag ttttaaatat aaaatttaga atcattctag
    35641 cataatatta tgtaatatag caaatttaat ttatagtatc aataaatata gactactaag
    35701 aatgtaaatg atttgttaga ttaaggtaca ttagatatgt aaatagaaaa ataaaaagta
    35761 tgttttaatt tatgtgatat aagttttaaa cagagaagca agtttgctct tacctctcta
    35821 tttaaaaata aaaaaatgtt ttttaagggc tattttattt aaatctttcc catctcaaat
    35881 catctccagt aaattctaaa atcctaaact tatctaatat tctagagaga gttctttgag
    35941 tgtaagtatt tcctatttga gatggtgtta agttggttga aataatgatt tttttaccag
    36001 caacaagtct tgtatttaca atattgaaga tttctccact agtaaaagaa ttattaagtt
    36061 cagttccaag gtcatctatt attaataaat cacaatcaaa caagttttta taattatctt
    36121 cacttatttg attatttgtg tctcttctaa atttataatc ttcaagaata tctaaaattc
    36181 taaaagaagt ttgatagatt acaacattgc ctttatctaa caattccttt gcaatacaat
    36241 tgcacatata ggtctttcct agtccagtag aaccataaaa cagtagattt tcactattat
    36301 ccttcttaaa atcatgaaca aaattttcgc agatacttaa attatttagc atattttctc
    36361 taggagatat ttcaccatca gagcctttct taggagaaaa tatattcaag ttaaaattcg
    36421 aaaaattttc ctgacttaaa atcctatcta aattagacat tttataagct tcatttacaa
    36481 tttcttgttt gaggcaagaa catttttctc cattaggtaa aaatccttta tctttacaag
    36541 aaatacattg aaactttatt tccaagtaat caagaggtat gttactttca gctaaaagtc
    36601 tttctttctt tacttttaaa gattctatat tctctttagt cttctttaca atttcatctt
    36661 tagacttagg atttaaaagt actatcttag ctaaactgag acctatttta gaaatttcat
    36721 catctataga ttttatctca gggattctat tatatacctc attttttcta tgttctaata
    36781 agagttcatt atcatctctt ctcttagcat atttagcaag tatttttctt attttatcct
    36841 cattcattta atcacctact taaacttttc tttttgactt tttttaataa tctcatcgag
    36901 ctcatcagag gtatactgag tgaatgtttc gttgaaatta tgaaacttag tttttttgta
    36961 agtattgttg ttttgagtag tattaatctg cttcttatta atattctcct taactatacg
    37021 ttcttcctct ttttgcttta aatcatttaa attttttata tttttttcat tccaattttt
    37081 aattatacca tttatataag atatacttgg gtttgaaata ttcttagact tagaacatgc
    37141 atttattatc aaatctatat ccatattaaa tttatcaaac caggtgtcca taagttcttt
    37201 ttctgatttt gatggttgtc ttgaaaatcc caattcattg aaaatcgttt tatataaaat
    37261 atatcgttca cttcttacca aaaagctttc ttcaacatct tttggcgtat ataaatttga
    37321 atcataccaa tttctaataa tgccttcaat gtatttaact ggctttgatg taccagtttt
    37381 atctttgacg tattcatatg cgcaaagtat catatcgggg ctcatattgt atttattcat
    37441 tatatccata atactcatct tttcacttgg gtcgagatat cgcccaacta ttttatttat
    37501 agaattaaac atttttctaa tacttggatt ttcagaagta gatacaactt tatctgtatt
    37561 agattttata gatgaattat tgcttaatat attctcaata tagaaatttt ttaaatctaa
    37621 aaattctatt gaataattaa aattatcata ttcttcattg tcatgtattt ttataatctt
    37681 cttttcttcc cagaatttcc aagcagataa cacatcagac aatggtatat ttaggttttt
    37741 ggctattgaa ttattgtcga acttaggatt agaagtaata tcacatgctt gtctatatcc
    37801 taaaagataa acttttacgt ataacccgtc cgccattggc ataaatatat ctataaagat
    37861 attgggaata gtgatttcac ctaaatctat ttcattgctt tctttaaaaa acattttatc
    37921 acctcagatt aaattatatc attttttata ttatatttgt ctgtaaacaa ataaaaggtc
    37981 acaagattag catgtaaata ttttctaaaa gtattcattt tgaaaaggag gagatatttt
    38041 ggttataaaa ttcaaccaaa aagtaagaag aacattatca gtagcgattc tatttatggt
    38101 attaataata ggttgcttta tagaaaagga tgacagttta gaaacataca aagaaaaaac
    38161 taattcaaaa ataaatcagt ataatatgga tgttatattt gatgatgaga ctaagaggtt
    38221 aatgtgcaat cagaatgttg actacataaa taatacaaaa tcaaacatag ataaaatata
    38281 tttccatata tatccgaatg ctttttccaa gaaagatttt gctccttttg aaaaaagtga
    38341 aatgacaagg gcttatccta atggatttaa cgaaggatat atagatataa agaatatatt
    38401 aaataataat agtaaaatgg agtataaaat taaaggagac aaaaatgaca tattagaaat
    38461 agaattaggg aaagagttaa aaccaaatga aagaatgtca ttagatataa aatataacgt
    38521 aaaaatacca aattcaattg gaagatttgg atatggagaa aatactataa atgttactaa
    38581 ttggtttcct atagcttgtg taaatgatga aaggggatgg aatttaaaga gttatgaaac
    38641 tattggcgac ccattttata gtgagactag cgattttcat gtgaaactat tgattccaaa
    38701 taaatataaa attgctcata caggaaaact tattgatgat aaacatgata ataagaaaat
    38761 gctatatgaa atagaagctg aaaacgttag agattttgca tttatactaa gtagtaaatt
    38821 tgatgtaaac aaagtagatt caaagggagt agctatcaat acatataatt taaataaaaa
    38881 gctttctaaa aaagcaactg aagtggcaaa agattcaata aatatattta gtgaattatt
    38941 tggaaagtat ccttacaaag aattttctgt tgtagcaagt gactttttta taggggggat
    39001 ggaataccct atgcttgtta tgatagacca aagtttatat aatgaaaaaa atgaattttt
    39061 actagaatat gttattgccc atgagacagc acatcaatgg tggtattctg ctgttggaaa
    39121 tgatgaaata agtgaaccct ggttagatga agcacttact gaatattcta caatagttta
    39181 ttttgaacaa aagtatggta aagatatggc aaacaagctt ataaaaacta tggagataca
    39241 gacaaaaagt tatttgagtg aaaatatatt taagccagca aatgagtata aaaattctac
    39301 tgaatacagc ttgaatgttt atacaaaagg cgcaatagca tttaatgaaa taagaaaaga
    39361 agttggagat aaagtattct ttgagacttt aaatgagtac tataataaat ataagcataa
    39421 aaatgctaat ggaagagctt ttgtggaact atggaatagt aaaggtgtag atataaataa
    39481 gattataagt gaatgtaagt aaatagtgaa tgtaagtaaa ttttgtgcag agtattaaat
    39541 tttatttaat actctgtttt tttggtataa tataatttat aaggcagaaa aagaggtgaa
    39601 ggaatgctaa agtattgttc agtagggagt ggtagtagtg gtaattgcca ctatataggg
    39661 tataaaaata ctaatatact agtagatgca ggattaagtg gcaagagaat aactacaggt
    39721 cttaaagaca ttggtgtaga tgcagataaa ttaaaaggaa tatttataac acatgagcat
    39781 gttgaccata taaaaggagc aggtatatta tcaagaaagt ttgatatacc agtatttgct
    39841 aatataaaaa catggtgttc tatgaaggac aaattaggtg acatcaaaga taaaaatatg
    39901 aaagtatttg aaaatgataa aacctactca ttaggtgata tcatagtaag acctttttca
    39961 atagaacatg atgcgtctga cccagttgga tataattttt atacagagag tgatgaaaaa
    40021 atgtctatag ctactgatat aggatgtata actcaaaata taaaaaaaca tctatataaa
    40081 tctaaattag tagtattgga atcaaattat gaccctaata tgcttatgat gggttcatat
    40141 acatatgctt taaagaaaag agtaatgtca agtactggtc atttatccaa tgaagatgca
    40201 gctaatttct gcgttgaact tattaatgaa ggtacagaat ctatattatt agcacattta
    40261 agcaaagaaa ataattttcc tgagttagcg tatgagactt caaaaggagt attagcatca
    40321 aatgatatag tagttggaca agatgtaaaa ctagatgtac tatcacgaaa tgatgtttct
    40381 aatgtttatg agatgaagta gaggtgaata actttgctaa agagaaattt tgaaaatgtt
    40441 gaggataata aaattttata ctctataaag cagggtatga ttttagcgat acctgctatt
    40501 atgactggtt caactgctct tgtaattttg aatcttccaa taaaggctta tcaagaatat
    40561 ttatcaggct tgtttaatgg agaggttaca aatattctta actttataaa tgattctact
    40621 ttaggaatta tttcattaat aatattatta acaataagct atagttatgg aaaaatatat
    40681 gggagcaagt atacagtact tgttcctata gttgcaatgt gctctttttt agtattttca
    40741 catggtaatg agcttaattc ttatatagaa atatttaaaa caaagtggtt atttacatct
    40801 ataattgttt ctatgacatc ttctgtattg tttgtaaaac ttacagaatc ttttgttaca
    40861 agtgtaaagt ttcatacaga aggggctgat gcagacttta atatggtagt ttcggctata
    40921 gttcctttca taatagtagt atttttgttt tctattttta gagtggtaat gataagtcta
    40981 attggcagtt caaactttca agatatattt tataatttat tttcaaatgt ttttaataaa
    41041 atgggaacaa accttatgag tgctctgttg tttatttttt taatgcactt tatgtggttt
    41101 tttgggattc atggtagtaa tgtattagat accgtcgcaa aaaatttatt tgaaaatagt
    41161 atggcaatta acataaattt agttaataat aatcaattac caacagaaat ttttactaag
    41221 accttttttg atacaatggt tttattggga ggatgtggct ctttactatg tttagtaatt
    41281 gcaatttttc ttagtgaaaa acgtataaat gtaagaaagt tagctaaaat tgcttcaata
    41341 ccagctcttt ttaatatcaa tgagatgcta gttttcggga taccaattgt ttttaactat
    41401 attatgtttg taccttttgt aattactcct ataatcttaa ccgttacaag ttatgtggct
    41461 atgtctacag gtattgtacc atgtacagtt tcagctgtag aatggacctc acctatattt
    41521 ttaagtggat atatgtctac aggttcaata agaggtagtt tgttgcaagt atttaattta
    41581 tgtgttggtg ttatgattta tattccgttt ataaaaatgt cgcaaaatag atatacatat
    41641 atgtttaaaa ataatataga aaatttaaca aacttattaa aaaggtatga attaacaggt
    41701 gaacagccta atcttctaaa ccataatggt aaaataggtt caatttcaaa aatgttggca
    41761 tcagacttga agtttgctat gaaaagagga gaaatagagc ttttttatca accacaagtc
    41821 aattataatg gaaatgttgt gggagcagag ggattactta gatggaaaca ttcaatagga
    41881 gggttcattt atccaccact agttataatt ttggctaaag aagaagggtt cttagatgag
    41941 cttggagagt atattattaa taaagcatgt attgatttaa agaagagtga aaagctattg
    42001 agaaatcctg ttaagttctc tgtaaatata tctccagacc aacttgatga ccctaagtta
    42061 ccagagcaaa taaaaaatat cattagtagt aatgatataa atcctaatat gttaggtata
    42121 gaaataacag agcaaattgc attatcaggt tcacaaataa ttattgaacg tatcaatgcg
    42181 atacataact tgggaataaa gttaattatg gatgattttg ggatggggca tagctctctt
    42241 atatatcttc aaaataataa ctttgatata gttaaattgg atggttcatt agttaaagag
    42301 atacttacaa ataaaagaag tagtgaaatt ataatgacta ttgtaaatct ttcaaaaaat
    42361 ttagattttg atatagttgt tgagtatgtt gaaaatatag accaaaggga taagttatat
    42421 ggtttaggat gtgaaatata tcaaggatat tattatagca aagcaatacc ttttgaagag
    42481 tttgtagaat atgcaaatca agaaagagag atataatttt ataagagctt gtaaattcta
    42541 gtaatgcata gatttgtaat aaaattaagt ttaggagaaa tatatgaata taagtatagt
    42601 agtagtaggt aaaattaaag aaaaatattt aaaattaggt attgatgagt ttaagaaaag
    42661 gctttctaag tattgtaagt tagaaattat agagttggat gatgaaaaag ctccagagaa
    42721 tttgagtgtt aaggaaatgg aaattataaa agacaaagaa ggaaagaaaa ttttagggaa
    42781 gataaagcat aatagttatg ttatagcctt ggctattgat ggtaagggtt tatcctctga
    42841 agagcttgct aagactatga gtgatttagc agttagaggt aatagtagtc tctgttttat
    42901 aataggaggt tctttagggc tttctgatga ggttttagga agggctgatt ataggttatc
    42961 cttttctaag atgacttttc ctcatcagat gatgagattg atattgttag aacagattta
    43021 tagggcttat aggataaata atggagaacc gtatcataaa taaatgatac ggtttttgtt
    43081 aatttgacac ttaataatat taaaataaca aaaaagtatt gaaagtatat tgcatttaag
    43141 gtagaattaa ataaaatatt ataacattat aatgtttata tggttgttaa taaaaatttg
    43201 gaggaaaata tatgataaaa ataatgttgg cctgttcagc aggaatgtct acaagtttat
    43261 tagttactaa aatggaaagt gcagctaaag aaaatggtat agagtcgcaa atttgggcaa
    43321 taccagaatc aactatacaa aatgaaatag aaaaatgtga tgtattatta ctaggaccac
    43381 aagttagata tgtattacct aaggctcaag aaattgctaa gccatataat ataccagtag
    43441 aagttataaa tatgatgcac tatggaacag ttaatggaga agctgtttta aatagagcga
    43501 tagaacttaa caacactaag taatataaat tataatgtat ttttaatata taaattggga
    43561 gaatcaaagg atggatgaaa agactataga aatatctttt aatataatag gttatgcagg
    43621 tgaaggtaaa ggattagctt ttgaagcaat aaaagaggct aagaatggaa atatagaaaa
    43681 agcaaaagaa ttattaaaag agtctaggga agttataaat aaagcacata gatatcaaac
    43741 agaattaata caaaacgaag ctagtggaaa caggactgaa ataagtgtca tacttataca
    43801 tgcacaggac cacttaatga atggcatgaa ttttcaacag ttagctgagg aaatcgttga
    43861 tttacatttg aaattacaag ataaataaaa attaaagata ataaattaag agagttctta
    43921 aaagatacat tatgtgtagc acactaattg ttagattatt gattcaatat ttagtgagta
    43981 atacattagg attattttga ggactctttt tatatttagt ttaaagttaa taaatattta
    44041 ttattaataa tataagatta tatgttataa tattataaca tataatttaa gaggtgaatc
    44101 atgttaagaa atcaaccatt atatatacaa gttgtagata tattaaagca aaaaatatta
    44161 aataacgaat ataaaattga tgaaaatatt ccaacagaaa gggagttaga gcaagagttt
    44221 ggagtcagta aaattactat tagaaaagct attgagatat tagaatcaga aggatatgta
    44281 gagaaaaaaa gtggaaaagg gacaaaagta ataagtaata gcctttttaa taagctatca
    44341 aaagcagagt ctttttcaag tatactttta tctaaaggtc ataagttatc taaagagact
    44401 atttcaattg aaaaaataat aaatgataca aattcacaag tatttagtat atttggagaa
    44461 aaatgtagca agattactag aatgtattat ttagatagta agccatatat attttttact
    44521 cattatattc cttttgatga aaatctaact gttgaaagtt ttaataatga aagttcttta
    44581 tatatgtact tatataggag aaatataaat ataactagat ttgaagatga attttttata
    44641 gaagaatcag aactagaggt atcaaaggct ttaaatctag ctaaagacat attattagga
    44701 agaaaaagaa agacctatga tgagaatgat aatatagttg agctatcatt tgcaagatat
    44761 aatacagaag tacaaaacta tgtaattaaa tacgaaatat aataagaatc ctgagtttat
    44821 tgaaatgata atatgctcag gatttttgtt tagttgtaaa aataaaaaat attataacat
    44881 tataatgttt acaaccaaac attataatgt tataatattt ttaataaaca agtgatatta
    44941 tcaattacat aaaaattagt ttatgttatg aaatcgttat tgcattataa ataaaggata
    45001 atatcaataa tacaggggga taactatgga aaaattcgca agttttttag agcgcaagat
    45061 tatgcctgta gcagcgaaga taagtaatca gagacatatg agagctgtaa gagcaggtat
    45121 aatagcaaca ttaccactaa caatagtggg gtcatttttc acaatattat taaacatacc
    45181 tattgatgct tatatggatt ttatagctcc atttaaagca atattagata taccatttag
    45241 atatactgta ggatttttat ctctatatgc tacttttggg atagcatcat cacttgctaa
    45301 aagctatgat ttagatacaa caggtgttgg tatgcttgca gttatggcat ttttagtttc
    45361 tacaatagtt ccaactcaag tagttgaacc agtaggtgga gttatagaag ctggtagatg
    45421 gatggatatg gcaaaactta attcgccatc attatttgga gctatcgtaa catcattaat
    45481 tacagttgaa atatatcgtt ttatgaagga aaagaatatt acaattaaga tgccagcagg
    45541 agttccagta gaggtttcaa attcatttac tgcattatta ccgacagcag taattttaat
    45601 gttattctgg gtaataagac atattctagg atttgatata agtgttgcac taagcgctat
    45661 gttatcacct ttaaaaggat tcttatcagg aaatagttta ggtggtgggt tactgacagt
    45721 attcctaata actttattct gggtacttgg aatacatggt ccagctataa tgggaccagt
    45781 aataagacct atatgggaca tgtctatagc agaaaatata actgcattca cagaaggagt
    45841 atcagctcat gcaatgccaa acatatttac agaacaattt ttacagtggt atgtatggat
    45901 agggggagca ggggctactc ttgcattagt tgtaatgttt atgttttcta agtcacaata
    45961 tctaaaaagt ttaggtagat taggattttt accagggtta ttcaacataa atgaacctat
    46021 aatatttgga gcaccaatag ttatgaatcc aatactaggg ataccattta taattggacc
    46081 tataataatg acaattctat catatgttct tactattaca ggggttatac ctatgatggt
    46141 agcaagatta ccatttgcaa tgccagcacc aatagcagca gtaatgagta ctaactggag
    46201 tatactagca ggtatcttag tattaatcaa ctttgtgata tcatttgctg tttattatcc
    46261 attcttcaaa gtttttgaaa aacaacaatt acaaagagaa caagaagaaa tgagtgaaaa
    46321 tttataaaaa tacatagata tagaggtttt gtactatata aaatatagaa aaagcttata
    46381 agttattaag ggtgaaggta ggaggaatga cctttgcctt taattttata tattttaaat
    46441 aatgtaattt gaggaggaga aagatggaaa gaagattagg aatatcagta tatccagagc
    46501 atagtaacat ggaagatgat aaggcatata taaaaatggc ttcaaaatac ggatttaaaa
    46561 gaatatttat gtgcatgctt tcaattacaa gacctaagga agaagttaaa aaacatttta
    46621 aagagattat aacctatgca agagaattag gatttgaagt aatccttgat atagctccaa
    46681 acatatttga tatattacaa atatcttatg atgatttaac attttttagt gagttaaatg
    46741 ctagtggaat aaggcttgac tatggatatg atggaagtaa agaagctatg atttctttca
    46801 atccatatga tttaaaagtg gaactaaata tgagtaatga tgtagcatac ttggataata
    46861 taattactta caagccaaat gtaaataata tatatggttg tcacaatttt tatcctcaaa
    46921 ggggtacagg cttgccatat gatttttttg taagatgttc aaatagattt aaaaagtatg
    46981 gaataagaac tgctgctttt gttaattcaa aatacgcaac aatagggcct tggaatataa
    47041 atgatgggct gtgtacgctt gaaatgcata gaaacttaga tattgctaca gcaaccaagc
    47101 acttattcgc aacaggattg attgatgatg taattgtagg gaatgcatat gcaagtgaag
    47161 atgaattaaa aagaatgtct gaagtaaata aatatcaagt tgtatttaat gtagtaactg
    47221 aagaaaaaac ttctgaagta gaaaagacaa taatggcaaa tgagcaacac tttagaagag
    47281 gggatataac caaccaagta gcaaggtcta cagaagtaag aaaaaaatat gctaaagaaa
    47341 atttccctaa gcatgataat acgaccatct ttaaaagagg agatgtagtg attggtaatg
    47401 atgactttgg aaagtataaa ggggaattac agttgatact tcaagattgt gcagatgaaa
    47461 gaaaaaataa ggtgggtaga ataaaggaag acgaaataat acttttagac tttattgaac
    47521 cttggagtaa atttaaactt attttagagg gagagtaaag tgaagataga catattaata
    47581 aaatgtggaa aaacagtaga taaaagtata attgatatag ctatattaga cgataaaata
    47641 atagaagtaa aaaatcagat agatgattct aagtatcagg caaatcaaat tataaaccta
    47701 gatggagaaa aatttattag tgctggttgg atagatatac atacgcactg ctacgaaaga
    47761 ctagaccttt ataaggatta tccagatgag gttggaataa aaagcggtgt tactactgta
    47821 gtagatgcag gaacaacagg agctttagat ataggtaaat tttatgaaga tactaaaaaa
    47881 tataaaacaa atgtatatgc tttaataaat attgctaagc agggaataac atctcaagat
    47941 gaactttcaa gtatgatgaa tattgatgaa tatgaactta gaagagcagt taaaaaatac
    48001 aaagattttg ttgttggaat caaagctaga atgagtaaaa gtgttgtaat ttcaaatgat
    48061 gttgaacctt taaaagtagc taaaagaata aaaaatgaac ttaatctacc tatgatggtt
    48121 cactttggga gttctcctcc aacgatagag gatatatttg actatatgga aaaaggagat
    48181 atattaaccc atatatataa tggaaagcca aatggaatac ttagaggcaa tgaggttaaa
    48241 aaagaaatca tagaagctag agaaagaggt ataatccttg atgtaggtca tggaaccgaa
    48301 agttttagta tggatattgc aatgaaatct aaggatgcag gaatttttcc agacactata
    48361 agtacagata tatatattaa aaatagaata aatggtccag tgtataatct atctacaaca
    48421 atggaaaagt ttatatatat gggctattca cttgaagata taatagataa agttactaaa
    48481 aatgcagcag atgcaataag tttaaaaaat aaaggtttaa taaaagaagg atatgatgct
    48541 gatttaacta tatttgatgt ggttaatgaa gaaaaagaac ttcaagattc tttgaataag
    48601 tcagtaatca caagtacgtc tataaagcca agagcagtag tagtcaatgg agaatattta
    48661 aatataggta agtctatagg agagaatgag tagaagggag aaactattgt atgagtagta
    48721 tatttgaaaa atactctgta agacgtgtta taaatgcttc aggcaaaatg actatactag
    48781 gagtatcaac agtagataag gaagttattg aagcaatagg tgaagggtgt aaaaatttct
    48841 ttataataga agaacttata aataaaacag gtgaatatat agcaaattta ttagggtgtg
    48901 aaagtgcact aatagtttct agtgcatctg ctgggattgc acagagtgtg ggagcaatga
    48961 tagcaaaaga tgatatgagt ctagtttaca atgtaaataa tcctaagaaa caagttaaaa
    49021 gggaaataat aattccaaag ggacataatg ttgattatgg agttccagta gaagtaatga
    49081 taagacttgg aggaggagaa gttgttgagg ccggttatgc taatatgtgt aacaaggaac
    49141 atataataag agagataaat gaaaatacag cagcaattat gtatattaaa tctcatcatt
    49201 gtgttcaaaa gagtatgcta agtgtattag atgcagtaca agttgctaaa gaatataatt
    49261 tacctttgat agtagatgca gcagcagaag aagatttaaa tgtatattat gaattgggag
    49321 cagacctagt gatatacagt ggtgctaagg ctatagaggg tcctagctca gggttagtaa
    49381 taggtaagaa aaaatacata gataatgtaa agcttcaatc aaagggtata ggaagagcta
    49441 tgaaaatagg aaaagaaaat attgtaggtc taacatgtgc aattgagaga tatataagta
    49501 atgataaagt aactttaaaa gaaatggagg ataagctaaa tccatttata gacaagataa
    49561 atactataaa gggagtgagt gctagtataa ctagggattc agcaggtaga gaaatacttc
    49621 gtggagaaat tgactttaat gaagatataa taaataagtc aacacaacaa attataaatg
    49681 aacttagaag tggagagata gctatataca ctagagacta taaggctaat gaaggcaaga
    49741 ttgagattga tataagaagt gtaactggaa gtgatttaga tactatttat aataggataa
    49801 aaaatatagt agaaagttag agtgtagagt aagtgacatt ataatgattt aataaattaa
    49861 attttaccaa tggaggggat aaatatgaat tgtataccac aatattataa aaatagagtt
    49921 tgtttaaatg ttcttgcagg aagcataaaa aatgctaaag aagtatatga tgcttgtgac
    49981 gggcatgtat tgataggaat actatcaaag aattatagca ctgtagaagc agctattgaa
    50041 gatatgaaaa aatactcaaa ggaaatagat aatgcaatat ctgtggggct tggagctgga
    50101 gacccaaatc agtcaaatat ggtatctcag atttctaaag aacttcagcc acaacatata
    50161 aatcaagtgt ttacaggtgt ggctacgtct agagcattgc ttggtcaaaa tgatagtata
    50221 ataaatggat tagtttctcc aactggtaaa ccaggatttg taaaaatatc cacaggtcca
    50281 ttaagttcaa aagaaaaaga tggtatagtt cctatagaaa cagcaatagc tatgatgaaa
    50341 gatatgggag gaagctcaat taagtttttt ccaatgggag ggcttaaaac aaaggatgaa
    50401 tatatagaag ttgcaaaagc ttgtgcaaag cataattttt atttagaacc aacagggggt
    50461 atagacttag ataacttcaa agagatagtt caaattgcac ttgatgctgg tgtagaaaaa
    50521 gttatacctc atgtttatac atcaataatt gataaagaaa caggtgaaac gaaagtggaa
    50581 gatataggag tactttataa aataatgaaa gaattattgg gatagattta tgaatatatt
    50641 agcttttggt gaaataatga tgagactttc agtacctgac tacaagttct taacacaaac
    50701 caatgaacta aattatataa ctacaggcac gggattaaat atattatctg gacttaaaaa
    50761 ctttggatac aatacctata tgttgacaaa gttgcctaat aataatgtag gtaaagcatc
    50821 aagtgcaaat ataagaaagc ttggagtaaa agatgacttt atcacgtatg gagggaatca
    50881 tataggagtg tattttcttg aaaatgggta tggagaaaga ccttctgaag tgacttattt
    50941 gaataggtta aatagttcat tttgtgagag taaattacaa gattatgata ttgataaatg
    51001 tttagaagaa atggatatag tccatatttg tggtattgct atgcaactta cagattcagt
    51061 taaagagatt gctttagaac tagctagaaa atcttatgaa aaggggataa aagtattctt
    51121 tgattttaat tttagacaat cattaaatcc agaaaggact tatgatgatt tagtaaatga
    51181 ttataagaaa atactaccat attgtcatgg gctgtttgct agttatagag atataaataa
    51241 gatacttaat atagatagtc taaatataaa atctagttca gaattgatta aaaaagagta
    51301 tgatatagat attgtagctg gaactatgag aagatttgat gatatgaata atagatttat
    51361 aaagggatat atatcaaatg atgatggtta ttttgaaagt agggagtata agctagaaat
    51421 atatgacagg gttggtgccg gagatggata tgtagcagga attatatatt catatttgga
    51481 aaatatgaag agtgaagata tagttgagtt tggggttgct agttcggtgt tagctcatac
    51541 aacttatggg gataattctt tagtaagcac aggtcatgta cttagactga tgaataatga
    51601 aaaaatagat atattgagat aatacatttg tatataataa aatttttatc ttttttagac
    51661 acatttttga cttgcttata tttgttaaac agagttattt ttaatgatta taaatattaa
    51721 attaaaagtt aaataataaa atagtagaaa atagcattaa ctatagaaaa aatggttaat
    51781 gctattttta tcgttaatat tgattaattt tatattatag aaatgaactt ttctaatgct
    51841 ggatttgtat tttttttatt ccatacaact acaacttcaa ttctattttc tacttctttt
    51901 ataggtacta ttgcaatatc acgttctatc attgtatctg taacacaaaa tgaaggtaaa
    51961 atggatattc ccatgttaga agataccaat gattcaatag tattaagatt atttgaacgt
    52021 tgtaccacat taggtacaaa ccctgcttca cgatatctat tctgtatcat tgacttttgt
    52081 ccatactcat cccctgtttc ctttacaaaa ataaatttat catgttgtaa ttcaattaaa
    52141 tcgatagaat cctcattgta aagtggatga ccaggaggta aaagagctac caaagaatat
    52201 ctttctacag ttttatattc aaaatctttt aatggttgat tttctggatt aataacaaaa
    52261 gcgaggtcca tagaattatt ttttactaag tttaaaagtt cggttatttc attagtacaa
    52321 atatccatag atatattagg gtatgtatta gaaaattctt ttaaatattg ttgaagtttg
    52381 tttttttcat atcctattaa ggttccaatg ctcaaagttc caaaaaaacc atgtgcaaat
    52441 tgttgtgttt ttgcaatagc atcattgata ttttttatta tagtacgtgc ctcagataaa
    52501 aaaacttttc cagctggtgt tagttcaaca tgacgtttac ttcttgtaaa tagagttaca
    52561 tttatttgtt cttctaaagc ttttatttgc tgtgtaacag cagtttgaga aatataaaat
    52621 ttttctgctg ttttggtaaa acttagattt tctgctacag atataaaata ttctaattgt
    52681 ttagtattca aataatcacc cctatttaaa cttgattgtt tgtaaacctt atttatctat
    52741 aattaaagct tattatagta taagaaaatt tacttttaaa gaatattaga aaatatttgt
    52801 ataaaaatat atgttttaag tatatcttta cattagattt tataaattta ttaataagaa
    52861 taacttatat attccaatga aaacgatatt ggatttaagt aagtttgtct cataaaatga
    52921 aagtatatca attgataata ctttaaataa gaagggagaa aaaatggaag ataaatttta
    52981 tgcaaaaggc aacggaaata acggatatat taaaaatctt gaagtttgtt cctttaataa
    53041 cttagatgga acttgtggaa tgtttcaaat ggctctgtac aaaagagatg aaaaatacta
    53101 tttatatgga tgctgttttg gaggaaataa aaaaaatgga gtaatgatta gcgatattac
    53161 agacccttat aatccacaat ttataaaaca ttttcaaatg ttagacccta aagagtatcc
    53221 tacaacaaca actcccaaaa ttcaaatagc agatgattta atgatagtag caatgagttg
    53281 tggaagtgga ccaggagcac ttgttgacca agctaaatta gcaaatatta agtgtgaagc
    53341 aggaattaga atatatagtt taaaagaaga ccctttaaat cctaagtttt taggatattg
    53401 ggattgtggc ttaaagcatg taatgggtgt tcatagattc atgtacaatg gtggaagata
    53461 tgtacattta tcaagtgatt gtgttggctt tgaaggtctg atttataggg tcatagatat
    53521 aataaatcct actaatccag tggaaatagg taaatggtgg agaccagacc aatatgcaaa
    53581 tggatatcca aatagaactt ttgatgcagg agcacctcat tgcccagaat ttatggataa
    53641 aggatggctt catggacctc catttgtaag agacggaaaa gcatattgtg gttatggagg
    53701 agctggttta gttgtattag atgttgaaga tttaacaaga ccaagatgct taggtgaatt
    53761 gccatttatg cctgcatttt ctagtagact tgcaggtgca agaactcata cagcattacc
    53821 attgccagga agagatttag tcgttgttca aaatgaggga gaaagattcc agttctttaa
    53881 accagataac attacagatg ttcaagctat gaataatata catatggttg atgttagtga
    53941 cccaacaaaa ccaacattaa ttgctcaatt tccatatcct gaagttccaa aagatttccc
    54001 ttatcctaac tttaatgttg cgggattagg aaaaccaggg ccatttggcc cacataatct
    54061 tcatgaacca atggataata agccatggtt agagcaaaga ggagatagag tatattgctg
    54121 ttatttccat gcagggctaa gggtttatga tgtatcagac ccatattata tcaaagagct
    54181 agcatatttt ataccaccaa atccaaataa aacaccagaa gaatcttatt tcccaggatt
    54241 cccaggacca cgcttggcag taacagaaga tcttatcgtt gatgatagag gctacatcat
    54301 catagatgct ttagatgatg gattctatat attaaaaatg aaagaagatt aaataaatac
    54361 taaagaggga gagttgaaaa tgaaaatact aggtatttct tttggaacaa aaaatggaaa
    54421 taatgacaca atgtgtaaag ttgcacttaa aggtgccaaa gaagcagggg ctgaagtaga
    54481 atttattcaa atgtcatcta ttaacattaa acattgtact ggatgttgtg cgtgtgttaa
    54541 aactttatta tctgggaaag gtagtatgtg tgtattaaaa gatgattttg aatggttgct
    54601 tgacaaaatg aaagatgcag atggtatagt agtttcagac cctatttttg aagaaggggc
    54661 atcaggatta ttccatacta taatggaccg ttttggaccc agagcagata gaggaaataa
    54721 cattatagga acaaaagttg cagaagctat aggaggaaaa attccagatt caagaatgct
    54781 taatgataaa gtaatatcat ttatgggaat aggaggttct gactggggta caagagttca
    54841 atgtgaacat gctatgctag ctctcatacc aatgtggaaa gttatagata atgcttggtt
    54901 cccatgggca aaggaattag taatggatga tacaaggctt gttcaagttc atcaaattgg
    54961 tttaaatata gttgaagcag caaaagattt tgaaaaagct tcatatcaag gagaagaagg
    55021 tgtatgtcca cattgccaca atagattatt ctaccttgag ccaggtacta aaaaggcaat
    55081 ttgtgctcta tgtggtatag ttggagagtt agatactata agtggaaaaa ctatatttag
    55141 ttttccagaa gagcaattag gtcatgctca tgatacttta ccaggtaagt ttatacatgg
    55201 tgatgatatt aagaaaatgg aaggtcatta tgcagaagtt cgtaaaacac aagaatttaa
    55261 agaaagaaaa acatcttata attttataga gccattaact aaagaaaagc aagctggagt
    55321 ttcattagga taataattaa aatactaaga ctctttttta aggtatgtta gatgttatag
    55381 gaggcttatt gtatgaatga aaagtcaagt tttaaacaaa ttgaaattat tttagcagtt
    55441 atttttttgg tacttacata ttacaaggtt agattaactc cagcatttat tgttatattt
    55501 ataccactat atttaagtta ttttgcagta tctatgattg taaatcaaat agcaattaag
    55561 ctattggaga taaggcaagc tagggaagct actaattaca atataggaaa tggatagaaa
    55621 gaaaaaagaa atattaacac tgtctatggg gattgcttta ctacctcctt tatgggcagt
    55681 attagcacct tatataggaa taaaaactgg agctgtagca ctaatctgtg caggattata
    55741 tgtaactaat ggtaataaac aaaaagatgg attaaaaata atgtttggtt tttggtgtgg
    55801 agatttatgg gcagtgttag ccattttaat tatgggatat atgaatttca atcaaaacct
    55861 agaattattt ttaacactta gtatattagg tttttttgca gttgtaattg catccttatt
    55921 tgaaaaaata attttcctac catcatggct ttgtggttgg gctataggtc taactattat
    55981 gacaggtgaa aatattatta atttacaaga tatttgtatt caaattgctg tagctatggc
    56041 agtaggagta tggtacgtgg gtgcaggtgt agatttgttt acaatatata ttttgagtaa
    56101 agataggaaa aagggaaatt gaaagcatgt gaatatactt tgattatagg agaatggcta
    56161 tgggttttga atttaaaata atgagaagtt taatatatgt aggacttgcc aaggaagaat
    56221 atagacctaa gctaatggac tggttatatc gtcaccatat tccagatagt attagcactt
    56281 ttggaccata ttgtactaaa tatgcctttt atcaagcata tcctacacca aatgaaggtg
    56341 agcgttttgg tgcacgtaag atgcaactaa cagaacatta ttggcttgta gatgaacata
    56401 tgcctgagat ggcaaataga attatgacag aatatatgcc tatggatgtt ctacgttggc
    56461 aagggtgtat accagatgta gaaaataaaa gggttcatga aaatgcagaa agtggagatg
    56521 caggacgtgc agtaggtgga gataatggat gtccaccatt tatatttgcc tttgttccaa
    56581 taaactggga agaagacttt agaggaaaag gacgtactgt acaagatgga ccaaactatc
    56641 gttggcaatt tatgattaag tatccagatg gtatctctaa agaagaagga gaaaaatggt
    56701 tctatgatga ggtagtgcca tactttacaa actgttgcta tgttaatcgt tttgtcagta
    56761 gtaaaataat gattaattat ggagcaactg cttttgaccg tgtatcagaa ctatggtttg
    56821 aaggggaaga agaatggtat aaagctgtgg ttgaagagac aaagtcgttt attaaaaaac
    56881 cagaatgggc acaagaagag gagttcccat atttaaaacc acaattcaat atcgcatcag
    56941 tattcttagg tgatatagca actatggatg catactcaca gtatcgtgga tatataccaa
    57001 tgagataaaa attatagttt gtgataagta gaggaggcat ttactcttac tggtagaaga
    57061 agacttattg tatccttaaa agtaggtgaa tatatagttt tatatttaaa tttatataaa
    57121 actaagacta ataaatatga aacaatagaa ttagaataat ttgataatat ttgttaaaag
    57181 agcatttaga atgtgtttaa agattaagat attcctttta tggtagcaga tagttgaaat
    57241 aataaaactg ttatcatgaa aggaatattt ttatgtatag gaaattttta aaaataaaag
    57301 ttaaatatgt ttaagaacat cttaaagtga aagaatcatt gttagaaata tgtagacaat
    57361 atagtcttta taacgtatca tattaacaag tttacacttg gattatgaaa taaaaagagt
    57421 aattccaaag gttgtaaata ctatgctgac tttgatactt caaagtttag gtgattttgt
    57481 aattgttaat agaaatacgt ttaatgagca taaagaattt atctaataat aggagaatat
    57541 aatgtataca attggacaag tatctaaatt tttaggaata tctagagata cactaaaatt
    57601 ttatgaggac aaaggattag taaatcctaa gaaaaatgat gaaaatggat atagaatata
    57661 taatcaggtt gatatctatg atattgcaac aataaacttt tatagagaaa ttgatataga
    57721 aattaaaaag attcaagaaa taagaaaaag taagagtata aacaatttag aactcttact
    57781 agaagagaag gaacaaatca tactaaaaga gatagaatat aaaaagcttc tcttaaaaaa
    57841 ggttcagtta gtcaaagaag attgtgacaa aataaaaaca tattttggaa aatacacaat
    57901 aaaagaaatg aagcctttgg aagtaaaagg taaaataaaa catgttacta actatgagga
    57961 atatgaagcc ttaaaagaga atacaaacaa cttaaaaaaa gcagttacat taactgattt
    58021 aagaagagta ataaatttta atgaagaagg tataatagaa gaaaaattta taattgtaag
    58081 ggaagtagat gactacaaca aaatcataga aggtgaagtt ctatctcatc ccaattgtat
    58141 ttatactatt attgaagatg gcagatggtc aactggagga gaaaatattg ataataaagt
    58201 ggaagacaga ttaagagaaa tagcaataga aaatgaatgt gaattacttg gaatagtcta
    58261 tgttaatata ttgcttacta cttatgaaga agggattgag cgtatatttt tagagattta
    58321 tgcacctata aaataatata aaagcattga cctgggactt actcataggt ttaaactcat
    58381 gatataagca gttataactt atatagcaat taaatattaa tttgaaatga ggattactta
    58441 tgagaacaga gaaatcatta aaaaggccta tagccagttt tattattcta acaaatataa
    58501 tttttttacc acttttttta ttggtagtag tgactataat gttaaaactt ccaacagtga
    58561 tatctgacat tgcgttgtgt atatcagcgt ggtcatccac ttttgcattt atgatattat
    58621 ttaaaaaaat ttatccaggt aagaaatttt tagtacacgt aaaagataga tttagaaata
    58681 aacttaaatt ttcaacagta tctataataa ttagtataca agtcctaata gctgtagtag
    58741 ttatatttat attagcatct aaaaatcatg gaataatgcc taactttaca gtttcatcat
    58801 ggggagcatt tatatattta tttttaaaaa atttatttgc aggatcatta ggtgaggaat
    58861 taggttggag aggatttgtt caaaatcatc ttcagaaaag acattctcct ttaaaagcat
    58921 ccataatagt aggtttttgg tgggggatgt ggcatttacc aatatggttt acaacaggat
    58981 tcaaaggact tgatttaata aaatatatta tattttttat gattgcaatt atatcaatca
    59041 gtattataat gacaactttt tataatctaa ataaaacttt gataatacct attgtaatac
    59101 atcaactatt taatttttta atagggataa ttaatggaga tttaatagag cttataaatt
    59161 actatgcaat gttatattta atatttgcaa ttgcattaat agttataaat ccaggaaatg
    59221 tattatataa aaatattaga gagtaatata tatctttatg gtaagatata aagatattat
    59281 tagaagaaca tccaaggagg aataaacatg gttttactga ttgttgatgc acaaaagtta
    59341 ataacaaacg agaggttata taaatttaat gaatttgtag taaatgtaga aaatttaatt
    59401 gatacagcta gaaaaaataa tatagaagta atatatgtac gtcatgatga tggagttgaa
    59461 aatgaattga ctaaaggtaa aaatggcttt gaaatatacg aaaaatttaa accatgtaat
    59521 ggagaaaaaa tatttgataa aaaagtaaat agtgctttta aagaaactgg tctattagaa
    59581 tacctaacaa ataatggtga aaaagatatt attattgctg gtctccaaac agattattgt
    59641 attgatgcca ctataaaatg tggatttgaa catggattta atataatagt tccagcttat
    59701 tcaaatacaa ctgttgacaa tttttttatg tcagcagaac aatcatataa atactacaat
    59761 gaatttatat ggaatgaaag atatgcgaag tgtatttcat tagaggaaac acttagaaaa
    59821 atgaaataaa aatagagtat gaattgtata tgtacataag ttgatttcat aaaaaaggat
    59881 gttaaattcc aagttaaaaa ttagattaaa tatattatat tatagataag gcacatcaat
    59941 tttattttaa attaatgtgc tttttaattg tagtaattta tttataatca atatgatttt
    60001 tattatttaa atcaattttt cttgagtttc tttgtctaaa gaaacttttt ttatttataa
    60061 aatctacagt tatattatag tatctatttt agagaagttt aatttttgta tcaatctaaa
    60121 acaaaagtta tacttaaatg aaattgaaaa cgataaaatt ctcaaataaa ataagtattg
    60181 tggaggtgat gaaagaatcc aatgataata tgaagactaa tcttaataag aaagtagggg
    60241 ttaatggtag tggacaaaga aattgtatca attctccaga ttttattaaa tagcacattg
    60301 attacaacta atggattaca agaggagtct aattcatcaa agagacaaat tacttataga
    60361 ataaataaaa tcaatgacat gcttaaagta aaaaatgtac cacttatatg tttaagagct
    60421 gataaagata ttatcgttag aaaagaaaca aaaaaagcaa ttaaagaaat tttagaaaaa
    60481 aactattcta agaatactta ttattttagt aagacagaaa gacttttata tatgtattta
    60541 atgcttttta ttaacatgga tgatatttca attaatcatt ttataaattc tataaaagta
    60601 agtaggtcta ctgtaaatct tgatttcaag gatttgatac cagagcttga aaagaagaac
    60661 attaaagtta agaacaatcg tataaatgga tattacatag ttggcaatga gatggaaatt
    60721 aggagagtgc ttattaaaaa tattatagaa actttgtcta atgagaaaag ttcaagagta
    60781 tttgatattt ttattaaaga atataattta gatagttttg aagaggctaa aaaaattata
    60841 ttaaagctaa taaaaaaata tgaaattact tttgttgaag atagattagt agaatttata
    60901 tatatattta tctttctaaa ggcaaggatg tattcaacta gaatagttac tcaggaaaat
    60961 gtagatattc ctaatatttc agttatgagt tcaatgaagg agtataaatt tgctaaggaa
    61021 ttattagaag tatatgggag tgaagataag ataaagcctc acaatataat gtatattagt
    61081 gcttggattt taggtatatc agtaggaaat tatgaagaag atacagaaga taaggatgta
    61141 atttcaaaaa ttgtaaataa aatgatgaca aagtttggat atttaagcgg agtttactat
    61201 ataagccaag aaaaaatatt taaacaatta tattctcatt ttagacctgc ctactataga
    61261 cttttattta aattacctat ttataatccg atttgtgaaa aagtaaaaga agaatataaa
    61321 ttagtttata gaattgtaag taatgcaatg aaagagtttt gcggattatt tggagaagaa
    61381 attcaggaag aagagttagc atatcttaca atgcactttg caacactttt ttcaaataaa
    61441 aaagaatttg atggttttag aaaaagaata gcattaatag tttgttcaaa tggcgttggt
    61501 agttcagcta ttttacatgc agaacttaca aacttatttc ctgacttaca ctttatacca
    61561 atgtcggaat cagttggatt agaaaatata agtgaacctg tagatattgt atttactact
    61621 aattataacg cagagggttt aaaaacagat gtaccagtaa ttaaagtaag ccctgttatg
    61681 accacaaaag aaaaatataa gattacacgt gaagtgtata ttcaattagg tgatattttt
    61741 ttaagacaac ctaaaataga tgaggttatg gatataataa aaaaatatgc aaatgtaacc
    61801 tctgaaagta ttctttcaag tgagctatta gcttatttta cacagataga gaattttaca
    61861 tctaaggaag gagaggggcc tatgctaagt gagattacta atgaaacact agtaaaaata
    61921 aaggtaaaag ctaagaattg ggaggaagct attagacagt cagcttctgt attagttgaa
    61981 aataacaaag taactgaagg gtatgtagat gctatgatta actcagcaaa agcatctgga
    62041 ccatacatag taattacaaa acatgttgca cttccacatg ctagaccaga agctgggtca
    62101 aaagagattg caataggcat tgctacttta gatagtccta ttgagtttgg gaataacgaa
    62161 aacgaccctg taaaatatgt attttgtcta agtgcaatag acaataatag ccatttacgt
    62221 gctatgtctg agctagttga actattggaa gaagatgagt tttttaaggt tttagatagt
    62281 gctaaagaag ctaaagaaat tattgatttt ataaaaacac acgaattata aaggaggatt
    62341 taattatgta taaaatttta atcgcatgta gagcaggagt tggctcaagt ttaatgttaa
    62401 aaataaagac tcaacaaatt attaaggaaa ataactttcc aattgaagta gaacatggtt
    62461 cattggattc actaaatgga ttcaatggag atgctgtaga aacattaatt gatgttgctg
    62521 aagaattaaa aaataaaaat ttgaagtttg atataattgg gattcacaat attgttgacc
    62581 gtaatgaaat aaagacagca ctagaaaaat tccttgcttc aaaagaggga taaatttaag
    62641 atgataagga ggaatattta tggagtttat tattagtttg ctgagtacac ctgctgtttt
    62701 gcttggcgta gttgcatgta taggattgat tcttcaaaag aaatcagctg ttgaagtatt
    62761 tacaggctct tcaaaaacac ttatcggttt tttgatattt ggtattggtg cctctgctat
    62821 gacaggagca cttcaaaatt tcaataaact gttccaacat ggctttggaa ttactggtgt
    62881 tatagcttca ccagaagcag ctacagcact tgcacaaggt acatatggtt ttgcagtttc
    62941 atgtacatta attcttggat tcatattaaa tttagtattt gcacgtatta caaagtttaa
    63001 gaatattttc tttacaacag gtcatagttt gttcttttct tgtgtattag ttttaattat
    63061 gaaagcacat ggatttgata atacaatcac tattttaata ggtggaacta tcttagggtt
    63121 tatgtctgct gcactacctc aattctgtca accatttatg cgtgaattaa caggtggcga
    63181 cgaacaagct attggtcact ttaacatgat tggttatggt ttatcaggtt atattgggcg
    63241 tttattttca aaacataaag atgatacaac agagactata gaatttccta agtggttatc
    63301 aatattcaga gacttcttaa tgggtctatc catagttatg ttaatcttat tttatattgc
    63361 aacattaaaa gctggtcaag catttactca agaaattgct ggttcaacac attggttggt
    63421 ttttccgctt atagaggcat ttacatttgt ggcaggtatg tcaatattga tgtcaggagt
    63481 tcgtatgttc ttagctgaaa taactgcagc atttgtaatc atttcagaaa agtatattcc
    63541 aggttcacgt cctgcgctag atgttccaac cgtatttcca tatgcaccaa atgcagttat
    63601 tattgggttt atatcagcat atgctgctgg tctattagca attgctatta tggcatcatt
    63661 tcctagtata tttcctgtag taattattcc agctgctcac atatgtttct tctcaggagg
    63721 tacagctgca atatttggca atacttcagg aggatggaga ggggctattg ctgggtcatt
    63781 tgttgtgggg ttattacttg catttttacc agttatacta tatccagttt atgggactct
    63841 tggtatagaa ggagcaactt tccctaatat tgattacaac gtagttggtt cgatattaca
    63901 taatatttta caatttataa aacctattta gactaaatta tataaaagta tagtcatagt
    63961 tactatgatt tcaaggagga tacatcttat ggaaaataca ttatctgaat ttttagataa
    64021 tatatctaat gaattgtcag aatttattgc ctcaatagat gagacatcta taaataaagc
    64081 atgcgaatta attcttgaag ctgaaaaaaa tcacggacgt gtacatgtga ctggtattgg
    64141 caaaccagga catgtatcgg gatatatttc atcattgctt tcatctactg gaacgtcagc
    64201 atatatattg catggtacag aagcagtaca tgggtcaagt ggtcaagtag ttgaaggaga
    64261 tgttgttatt gcaatatcaa atagtggtga aactcaagag ttaaaagcaa ctcttaagac
    64321 tctaaaagta aatggagcaa agataattgg tgtaagtggt aatgagagtt catttttaaa
    64381 aaatatttct gatatattcc tgtttgcagg tgttaagcag gaaggagatt gtctcaataa
    64441 agctcctcgt gcttcaattt tagctgaaac aataatatta caatcattaa gtgttgtatt
    64501 acaatatgct aaaggtctta atacacagga gtatttgaag tggcatccag caggaagtct
    64561 tggaaagtct attagagagg gaatataatt ttatgaaata taaagggatt atatgtgcaa
    64621 tgataacacc atttgatgaa aatcaaaata ttaattcaca agcaacatgt caattaattg
    64681 attatttgat tgaaaaggga atttatggat tatttatttt gggcactaat ggtgaatgtc
    64741 atgtattaac agatgatgaa aaagttgaat ttgcaaagat tgttattaat catacaaata
    64801 atcgtgtacc tgtttttgtt ggaacagggg gtaattcaac tcgtgaagtt gtagatttat
    64861 ctaagagaat ggaagcgatt ggagcaagtg cattatctat tattacacca tactttgtta
    64921 caccaacaca acaggaatta atcttacatt acaaaactat tgctaattca gttaatttac
    64981 ctattataat gtataatatg ccaggtaaga ctggaattaa tattgaacca gagtcagtac
    65041 gtgaattatc aaagataaag aatattgttg gaattaagga tagtagtggt aaacttgaca
    65101 acatgaaagc ttatattgaa gcaacgaaga atgagaattt tagtgtgttt tcaggctcag
    65161 attcacttat tctggatagt ctaaaagctg gtagccaagg agctgttgca gcaacaacaa
    65221 acttcctcac agagattggt atctctatct atgaaaactt tatgaaaggg gatttggaag
    65281 aagctcaaaa atctcaaagt agtatagaag aacttcgacg tatactgaaa cttggaacaa
    65341 ttccatctgt tatgaagaaa actattgtct tgaatggaat taatgttgga actgcacgac
    65401 ttcctgtaac tgaaccaact ggggaagtac ttgaagaaat taaacgagta gttgaaaatt
    65461 ataggacaat acttaataaa agtagtgaga atagataata taaataggga aattacactg
    65521 gtatataatt aatatatata ttataaaaca tgactaaaat taatgaataa gtaaagtggt
    65581 ttgatgagta tagaggtgat aatatgaaaa agatgaatat aaccaaaaga atgtcagaat
    65641 gtggggctct tgcaattgta agagaagaaa atcttaaccg tgcatgtgaa attgctgaag
    65701 gatgtataaa aggtggtata acagttatag aaatgagtta taccctaaat aatgctggag
    65761 aaataattca agggcttaat aaaaagtatg gtgaaatact ttgtgtggga gcaggaacag
    65821 ttttggatag tgaaacagct cgtcatgcta tattacatgg agcccaattt attattgcac
    65881 caaattataa tgaaggtgta gcgaagattt gcaatagata tcagattcct tatgctccag
    65941 gatgtacatc attgactgag gcagtagacg cactaagttt aggagcaact tttataaaag
    66001 ctttcccaat atcagatttc tatggaccta aactagtaaa ggtatttaag acaccaatac
    66061 ctgatatgcc agttttagca agtggagggc ttaatattga aaacctacat atatggttag
    66121 aaaatggagt tgatgtatgt ggatttggtg gtttattaac taaaggttct gttgaagata
    66181 ttgctaaaaa tgctaacaag attcgtgaaa tcattaaaaa tgtaagaaaa atttaacata
    66241 gggctgtatc atagtatctt ataattacta tgatacagcc tttttaatat tattcaaatt
    66301 taaaacagtt tacatttcta ttatattaca tataatttca gtaattttat cttaattttt
    66361 ctatctggaa aaaaatgatt taaaacttaa gttatgatat aatttcatta aaagaaggcg
    66421 atttttagta aatatgagaa aataatgtgt ttttagggga aaagttaaat acagatttaa
    66481 gtgaacaaga atcgataatt aagatattaa atggtgaata gggcatcaaa aaaattacta
    66541 aattaaaagt tttagaggga gaatcttagc aagtgtgtgt gaaaattcaa tttcaaacaa
    66601 aaataattgt aaatgaaatc aatttataca cgttgaagat tcacttttat cattatatta
    66661 aaaagcagag tgaattaaaa gtgagtctaa agataggagg ataattaaaa caatgaaaga
    66721 agacattagg ttacatcgaa tgtgtaaaaa agaacaagca acaaagaatg aagttatatc
    66781 agaggatatt ctaaaatcta tgagtattgg ggtcagtaaa cattttttag atgattcatt
    66841 tacaataata tggggaaata catcattcta tcaattatta ggatatacag aagaagaatt
    66901 tttagttaaa ttttctagtt taaaagatta ttatgaacag aatctttatc attttgaatc
    66961 tatgaaaaat cattttttag aggcttatca tcatggcgaa aagagtatag aatatcaaat
    67021 ttgcatttca gcaaataatg gagaccctgt ttgggtaata atgactggta tattaattga
    67081 atcacaagat aatgagaaat caatggtcta tatattgtat tcaaaaatta atgagttaaa
    67141 aataaagcaa gaagaactat ctgatatgga aaaagagagt acagagaatt ttaaatggat
    67201 gatgtcagag tatgctggga atgtttatat tagcgatatg gatacatatg aattactata
    67261 cttaaataag cactcatgca atactttaca ggcatcagca aatgaactta ttggaagaaa
    67321 atgttatgag gtaattcaag gtagaacttc gccatgtccg ttttgtacaa attcgtattt
    67381 aaaaaaagat gacatatatg aatgggaatt ttataataaa aaactgaaac gaacatttat
    67441 aattaaagac cgtatgttga attggcaagg gcgaagagtt cgtattgaat tatcttatga
    67501 tatgtatagt gctgaatata aattagcaaa aaaagatcag gaaagagaat caattttgaa
    67561 aactattcca gcaggaatgg ttagattaga tgcaagagat tatcttacta tactttggtg
    67621 taatgatata tttttgaata tgattgaata tacaaaggaa cagtttgcag aggaacttaa
    67681 taatcaatgt ggatatttat tttctgaaga ttataaacgt agtaagaaac tggcacaaga
    67741 tttgaaggag tctggggata atgttgtatt tgaagcaaaa atatatacac gttcaaggga
    67801 agaacgtata tggactgtta cattgtgtta tatcagtggg gaagacagtt gggatggaat
    67861 accatctttt tatagtatag gtattgacat aacaaaggaa agaaaacaga ttgagaaatt
    67921 acagcatata tcagagaaag atgcattaac aggaatttat aatcgtgctg agactgaaag
    67981 gcagattaag aaatattttg aaaaaaattt aaatgttatg ggtgcactgt ttatgattga
    68041 tacagataat tttaaacaga ttaatgatac agaagggcat atgattggag atatagtact
    68101 tactgagatg gcatcaggta tgaaaaaaat aatgcgtgac agtgatgttg ttggaagaat
    68161 aggtggagat gagtttacaa tttttatgaa gaatatttcc tcagttaaag atgcagaaaa
    68221 gaaagcagaa gaactattgt atatgttccg tcatctattt cagaaagaaa aaagttttct
    68281 aaaagtaact tgtagcatag gaatagctat atatccaaag gatggtacta cattcaagga
    68341 aatatatgca agagctgata aagccctgta tcaagcaaaa aatatgggaa aaaataatta
    68401 tgtaatctac aaccaagatt attttaaaga attagaagaa tttgattgtt cttctcttgg
    68461 tacagttatt gattcagaaa aacaatacgc tgaatatcct gataatctta cacgttatat
    68521 attccgtatg ctttatcaga ctgatgatat agaccaggcg attaatatga tacttgaggt
    68581 tgttgggaaa cagtttgatg taagtcgtgt ttatattttt gaaaatacag aagatggtag
    68641 atatacttct aatacttatg aatggtgtaa tgaaggaatc tcttctcaaa tggcttattt
    68701 gcaaaacagc aattatcaag actatgagga ttatgaaaag ctttttgagg atgaattagt
    68761 tttttattgt cgagatatcc atacattaag tcctaaacta gaagaattgt tttctagtca
    68821 aggtatccac tctacacttc aatgtgcata taaagaagat agagttttca gtggatttgt
    68881 tggatttgat gaatgcacag gactacgtct atggtcacaa gaggaaataa gtacattatc
    68941 attgatttct caaattatat caatgttttt acaacgtaaa aaaaataata aacttaatca
    69001 acagattcaa caataccaga atattttaga taatattgac cagtgtattt gtgtgattaa
    69061 taaagaaaat aattttttat tatatgtaaa taaaaaattt aaaaatacat attctaaatt
    69121 ccaaattgga caaccttatt atcttgattc tatagatatt cataagatat ccattatatg
    69181 gaataaaaaa gaagcatacc tatgtactag cacgatttaa tggtaaagta aagtttatat
    69241 tggacttaaa ttaaatttcg ttttaaataa gatattattt tagttgctta ctgaaaagac
    69301 tagaatctat attaatgaat tatactaatt ttataccact attaaaatat tgtttgctaa
    69361 aaataaaaaa tagccttaaa acagttgatt ataaaaaatt aactgcttta aggctatttt
    69421 tatctttgtg acgaaataga cacttgccat ttagaatgtg acaatatagt agttgtttag
    69481 aaaaggcaca aagcattata ctttaactaa agtaaaatgt attttgcttt tagaataaaa
    69541 acaattagga gggagctata attgaaaaag ataatatatg tattatttac aattgaacta
    69601 ttaatagtat ctttattagg tctaaatata gttaaagata atgaaattaa taatatttta
    69661 tataacgata caacaagtat atctgtaatg tttaaagatt ataaaaagtt aaataaaaat
    69721 tatagtaagt ggattaaaga tattgcagat gaaaataatg tggctatatc aaagtatgtt
    69781 tttaataata atgaaaaatt atctatatat acgactgata caagtttgaa taacaatata
    69841 aagttaaaat caggaaaatt tcctaatact aattcaaatg aatttattag taatataaac
    69901 actaataata gtaatcaagt aggtaaattc tcaactatga gtaaagacat atcaatttta
    69961 attagggatt ttggtaaaat aagtgaagtt ggagaatcag gaatcttata tatttcaact
    70021 caaaatgaag atacaataaa taaaatatta agagaactaa atgtagacag aagtattttt
    70081 gtagcaagat tacatgatag atatgttaac tcaaatttat acttaaatcc ttcaataatt
    70141 agagatttaa tactaattat cctttgtttc ttggctacaa taatacatta ttcaattagt
    70201 gtgagtagag aggcttctat tttaagatta aatggatact ctaagctaga tataattgct
    70261 agagttataa aaaatatact tagaattatg attttatcat ctgtcacagc attattaata
    70321 tatataatct atattttaaa attaaatata ggtgttagag catgcttata ctttgctatg
    70381 ttttcgatta tatgtatttt gataaatatt ttgatatcta tattaatagt tggttttaat
    70441 agtagaagtt ctaaatatgt gctatctcta aatggaaaaa aatcttatgg ttttgtaaat
    70501 ataatgcatt tcacattgaa aattgtattt gtatcatttc ttatattatc cataaataac
    70561 tgtattttaa attataatat gctacaaact cagttaggaa atctatctga gtgggataaa
    70621 gcaaaaaatg tttataatct tactcttaaa gatacaggcg aacaatcttt aggaaaggaa
    70681 gaagtaatta aaaatgccaa gattaaaggt ttctataaaa atttggtaga agaaaaagat
    70741 gctttcttaa tagattcaac taattttgaa aaacttgaag atggaagtta catgtatgaa
    70801 ataaattcaa agggtaaaaa cccagaagta agtcaatatg gtaaaaatat aaaaataaat
    70861 aaaaactatc ttaaagttaa ccctatttac tcaaatggca aagaaatatt taatctaatc
    70921 gattatgacg ataagactat aaatttatta gttcctaaaa agttacaagt acatgaaaat
    70981 gagattaaaa aagagtttag agaattattt tattttgaaa aaatagaagt agaaaatatg
    71041 tacaataaag agttaaatag agagttaaat aaaacaaaga agtccgattt aaatgttaat
    71101 ataatatatg tagataataa tcaaagctat tttacttata actcccttgt tatggatgac
    71161 aataggaatc tcattgatga cccaatagca atagttgaca tagataatat tgatgattct
    71221 ttttatcttt cttatatatc tagatgtgta tattttaatt ctaagaaatt agatgccctt
    71281 gcagatataa gtagcattat tgaagctcaa ggtgtagaag cgcatatcca aagtttacat
    71341 gctgtttata atgagtatgg tttagaaata aataaactag aaaaatattt gaatagtgaa
    71401 atttttacta taataataat agcaatatca aacttaatga ttacctacaa tatagtagct
    71461 agctactatg aaagaaataa atataagtta tatattaaaa aaatatttgg ttactcaact
    71521 acagctagaa gtatgttgtt aatagtctca ttaatattaa caaacattat tccaataggc
    71581 attatatcaa caagagtaga tttaagtaat aatattatct tatttggact tctaatatta
    71641 gttatagaag taattgtatc tattgtatta gacaagatta tctcaaatag ttcatttaat
    71701 aaaattataa aaggagaaca ttaaaaagat gatttcatta ataaatatat ctaaaaaatt
    71761 taaagataaa tctataatag acaaatttaa cctagaaatt tctaagggcg agtttgtagc
    71821 gataacagga aatagtggtt caggtaagac aactttactt aatataatgg gattattaga
    71881 aaaacctaat tctggtgata ttataataaa taatataaaa aatccaaata gtaaacagat
    71941 aagaatttta caaagggatt tttatggcta tctatttcaa aattatgctt taattgagaa
    72001 tgaaaatgtt gaaaataact tgaaaattgc tttaaagtat caaaaaaata tcaatcataa
    72061 tgaaaaaata aattacgcac ttgagtctgt aggtcttaga aaatacaata aaaaaaagat
    72121 atacgaattg agtggaggag aacaacaaag ggtagcatta gcaagagtaa tattaaaaga
    72181 atgtgaagta atatttgcag atgaacctac aggtaattta gataaaaaaa atagagacat
    72241 agtatttgat atacttaaaa gtctaaataa gaatggtaaa actatcgtat ttgtaactca
    72301 tgatttagat ttatcggagc aggcagatag agttataaaa ttgtaataat ataaactata
    72361 ataaatataa gaaagagatt acagctaaat taagtttaat aataatacga atagaattta
    72421 aacattaagt aaattagtag tttgtaaata taagtataat atgatatcat aaaggctcaa
    72481 aagaaatttt atataataag aaatctaaaa ataacaaatg gaggacatat aaagatggaa
    72541 aatttaccaa attgcccgaa atgtaattca gaatatactt atgaagatgg aactctttta
    72601 gtttgtccag aatgctctca tgagtgggca ttaggattgg gagctgaaga agattcaaat
    72661 attatcaaag atgtaaatgg aaatgtatta aatgatggag attctgttac agtaattaga
    72721 gaccttaaag taaaaggaag ttcatcatct ataaagattg gaacaaaagt gaaaaatatc
    72781 cgtttaatcc atgactcatc agatggacat gatatagaat gtaagataga tggttttgga
    72841 gctatgaagc taaagtcttc tgttgttaaa aaagcgtaat attataaata agctgtagtg
    72901 tttattgttt ttcagtacag tataaaacca taagaatcta ttaagtaata aaagtttgtt
    72961 tatttatctt tatattttct tatggtttta ttaaatataa attttattga acaccaaaat
    73021 attctctcct ctacaaataa gccaatctta tttaaactaa tatactttgt actatttata
    73081 ctgaaattgt aaatttaaat gctatgttat ttaggtttgt atagttaatt tatatatttc
    73141 tgattttata aattgtattt attattatat cttgattcta aaatcattaa aaatttcaac
    73201 ttctataaag atttacaatt tatcaaatac attatgagta tataaacaat ttattattac
    73261 agtgctacat ttaaaataag tcttagtata aaggtattgt tacaagtata aagaaactta
    73321 tgtatgctat tggaataaat aataccttaa attataatta agaataagaa ctgttaaata
    73381 agggacttga ttatcattta gttatagata atataatatt aaaaataaag tttaagagga
    73441 gaaaaaatat gatagatata cataaaaata tatatgataa taagttattt gaggagctaa
    73501 agattgactg taagaagtgc tttggacttt gctgtgtagc tctttatttt tctgcttcag
    73561 atggatttcc tatcaataag gaatcaggaa aaccctgtat taatttacaa ccagatttta
    73621 aatgttctgt acataatagt cttattaagc gaggttttaa agggtgtact gcatatgatt
    73681 gttttggttc aggtcaaaaa gtagcacaag taacatataa ggggatagac tggatgcaat
    73741 caccagaact aacaaatcaa atgtctgaag tgtttttaat tatgagacaa ttacatgaaa
    73801 tgttatggta tcttaaagaa gcatctgtat taaatatatc agatacaatc aaatcaaaaa
    73861 ttgatttaat aattgaagaa actgaaaaga taactaatat gggcccagaa cagataatta
    73921 atttggatat catatcacat agaacaaagg ttaatttatt attatcacaa gcgagtgagt
    73981 ctatgatggg aaaggttaaa tcatttatca agacttctac tcttaaaaac atgaaaaaat
    74041 taagtaaaaa tatagattta ataggagcag accttagaaa aataaatcta ataggagcag
    74101 atttaagagg tagatttcta atagcagcaa atttaagaaa tacagatttg agtggggcaa
    74161 atttaatagg agcagactta agagattgtg atattagagg tgctaatctt gaaaatagta
    74221 tatttttaac acaattacaa gtaaatactg caaaaggaga ttctagtacg aagttaccag
    74281 cttcactaat acgccctaaa tactgggaaa aataattaaa ttaatacata attttacaaa
    74341 atatgaactt ttttgacttt aattcatatt atacattaga gattctttac aagaatttct
    74401 taatataata tagggagaga tataaaaatg agttatcaaa attataaatg tgaaagagat
    74461 tatgtgtatg atgattataa atacaataag tgcaacaagt gctataatga ttatgaggaa
    74521 atgacacatg ttcatgaata ttcagaaagt gttaagttag cagaagaatg tgaggataga
    74581 cataaccacc gtgcagcagg ggttactggt gaagttatac caataaatgg aggtacaaat
    74641 catgttcata aaataaatga taatgtagat ttccttgacc atttccataa gatatgtgtt
    74701 acaacagggc cagcaattag aatacctgga actgataaac acattcactt aatatgtgga
    74761 gaaactactg ttaatgatgg tcattgtcat aaattccttt ttactactca aatagaagca
    74821 cctttagtat aagctgatgt gaattattgt agtgaatttg ctagattata gtataatata
    74881 taatagataa tatttaaact taatgaggac tatgatattt aaccttcgta gtcctttcta
    74941 agttttgtat aaaaaagtgt tatgcaatta aaagtgatat agggaggaaa tttttatgaa
    75001 atctggaata attcttgaaa tgaaacaaat aaattcaaca gaatatgacg aaatgttgca
    75061 agtctgggaa tcatcagtaa gagcaacgca tgatttttta acagaaaaag atattgaatc
    75121 tttgatacca ttagttgaga ttggacttaa agaagtagaa aatatagttt gtataaaaga
    75181 taatgatata ataaagggct ttataggaat agataaagat aaaattgaga tgctctttat
    75241 agaagataaa tatagaggaa atggtattgg taaaaaatta ataaaatatg ctatagataa
    75301 atacaatgta aaatatgttg atgtaaatga gcagaataaa aaagctgtag gattttatat
    75361 tcacttagga tttaaagtgt ttgataggtc tgaaatagat ggacaaggga acccatttcc
    75421 aatactacat atgaaattaa tttaaagtta tacttcccac ttattcgcaa aaatatttac
    75481 ttaaattttt atacaaataa aagtcgtgac agtcttagga cacgactttt ttactacact
    75541 attaaattga aatttatctg ttctatatta tgattattag atgtagtttt tttattttaa
    75601 ctatgtactt aaataccaag tatacccata cctatcaagt acttcagcag cgccatcatt
    75661 ttgatgtggt ggtgggtgtg gtaaaaattc ttgtacaagt gtaccacctt cggcaagttt
    75721 tacagatatt tcttctatcg cttctcgaga tggtagaaca acacaaatcg ccatgccaga
    75781 aattacaaca ggcttgtctg tatccgcacc cattatacaa ccttcgtatt ttgtgaaagt
    75841 aacacttgcg tgcataatag gattgtttgg attgctgtct aatcgcgcaa atatagtttt
    75901 tcctcctccg aaagcctcag cataaaaatt aaatgctttt tcacaagtac cattaaatat
    75961 taaatatgga gttagcatga ccaatatatc ctccttgata tagaataatt tatagttgca
    76021 cctgataatc taaattatga tttagtaaat tgtatcatag ggaaaccttg aagtatataa
    76081 ttttttatta ttatattata caaatttatt aatttaaaaa aatgttatat gtacggaagg
    76141 tttacaggat aaaatataat aaaaagatta aggtgacatt ataaaaaatg tatattgaca
    76201 ttgacgctac gtaattgtat atactttgtc ttaaaggaga tggtaaatat gaatattata
    76261 gaaattcgct cagataaaat atacaagaag ataatggatg caccaataaa caaaaaagaa
    76321 gatatataca gatatgaatt aatgaagcct tttgaattta agtggaagtg tatgaatgtt
    76381 ccaatagttg ctagacagaa aggtggatat gatgtaatta tagcaagtga aatgttaggg
    76441 gttttatcgc ctaaggatat tgatgaaaag caaaaaaaga atataaatgt gttatctgct
    76501 gataaaattt ggggcacttg caaagaaacc atagaaaact ctataaatgc ttttataaaa
    76561 gaagggtatg atttaaacat taaggactat aaatattcaa tattattggc gaatccaaat
    76621 agtccttata caatattaag tgatggatac tggggtgatg gtgggattcc tggatatata
    76681 tttctatcat tggttcctaa tgaatatact atcaatagat taccagtatt aatagcacat
    76741 gaatgtaatc acaatattag atttcagttt atagagtgga ataataatat aacattagaa
    76801 gaaatgatga taaatgaagg tcttgcagaa aattttgcaa catggatgtt tggagaggaa
    76861 atgttaggac cttgggtcag tagaacagat atcgaaacat taaatactta tataaagcca
    76921 ataataaaaa gtgctttaaa agaaactgga tttcaaaata taacatctta tctttatggt
    76981 gatgatatag ctaaaatgca aggatatttt ccagtagggt tgccttattg tgcaggatat
    77041 gcttgtggat attatatgat taagtattat ttagaaaaga caaataaatc aataatcgaa
    77101 gcgactttat tgccttatag tgagataatc gaagcagtaa aagagttttg ggaataaatt
    77161 ttataaattg cataatttga aataaattat gcaattattt tatttttaag aaaaaataaa
    77221 ttgacaataa tttataagaa atatacaata atatatataa agaaatattt caatatttaa
    77281 aagttttaag atttcatatc tagagtagct aacattacat gttagtttct tgttactta
//
LOCUS       DAYATN010000004        72850 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.004, whole genome shotgun sequence.
ACCESSION   DAYATN010000004 DAYATN010000000
VERSION     DAYATN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 72850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.004"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            117..1313
                     /locus_tag="ACGI8A_000240"
     CDS             117..1313
                     /locus_tag="ACGI8A_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFH0178252.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1406..1930
                     /locus_tag="ACGI8A_000241"
     CDS             1406..1930
                     /locus_tag="ACGI8A_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFH0178253.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1955..2131
                     /gene="rpmF"
                     /locus_tag="ACGI8A_000242"
     CDS             1955..2131
                     /gene="rpmF"
                     /locus_tag="ACGI8A_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFH0178254.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2338..2895
                     /gene="fapR"
                     /locus_tag="ACGI8A_000243"
     CDS             2338..2895
                     /gene="fapR"
                     /locus_tag="ACGI8A_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HFH0178255.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2906..3928
                     /gene="plsX"
                     /locus_tag="ACGI8A_000244"
     CDS             2906..3928
                     /gene="plsX"
                     /locus_tag="ACGI8A_000244"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFH0178256.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3954..4940
                     /locus_tag="ACGI8A_000245"
     CDS             3954..4940
                     /locus_tag="ACGI8A_000245"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFH0178257.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5049..5978
                     /gene="fabK"
                     /locus_tag="ACGI8A_000246"
     CDS             5049..5978
                     /gene="fabK"
                     /locus_tag="ACGI8A_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HFH0178258.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5994..6944
                     /gene="fabD"
                     /locus_tag="ACGI8A_000247"
     CDS             5994..6944
                     /gene="fabD"
                     /locus_tag="ACGI8A_000247"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFH0178259.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6944..7693
                     /gene="fabG"
                     /locus_tag="ACGI8A_000248"
     CDS             6944..7693
                     /gene="fabG"
                     /locus_tag="ACGI8A_000248"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFH0178260.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7747..7971
                     /gene="acpP"
                     /locus_tag="ACGI8A_000249"
     CDS             7747..7971
                     /gene="acpP"
                     /locus_tag="ACGI8A_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFH0178261.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            8000..9238
                     /gene="fabF"
                     /locus_tag="ACGI8A_000250"
     CDS             8000..9238
                     /gene="fabF"
                     /locus_tag="ACGI8A_000250"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFH0178262.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADLMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9464..11182
                     /locus_tag="ACGI8A_000251"
     CDS             9464..11182
                     /locus_tag="ACGI8A_000251"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0178263.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11213..13213
                     /locus_tag="ACGI8A_000252"
     CDS             11213..13213
                     /locus_tag="ACGI8A_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0178264.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13564..13809
                     /locus_tag="ACGI8A_000253"
     CDS             13564..13809
                     /locus_tag="ACGI8A_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HFH0178265.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13844..14569
                     /locus_tag="ACGI8A_000254"
     CDS             13844..14569
                     /locus_tag="ACGI8A_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFH0178266.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14591..15904
                     /locus_tag="ACGI8A_000255"
     CDS             14591..15904
                     /locus_tag="ACGI8A_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0178267.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            16110..16649
                     /locus_tag="ACGI8A_000256"
     CDS             16110..16649
                     /locus_tag="ACGI8A_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFH0178268.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16738..18723
                     /locus_tag="ACGI8A_000257"
     CDS             16738..18723
                     /locus_tag="ACGI8A_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HFH0178269.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18947..19915
                     /gene="spoIIIAA"
                     /locus_tag="ACGI8A_000258"
     CDS             18947..19915
                     /gene="spoIIIAA"
                     /locus_tag="ACGI8A_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HFH0178270.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19905..20426
                     /locus_tag="ACGI8A_000259"
     CDS             19905..20426
                     /locus_tag="ACGI8A_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HFH0178271.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20443..20640
                     /gene="spoIIIAC"
                     /locus_tag="ACGI8A_000260"
     CDS             20443..20640
                     /gene="spoIIIAC"
                     /locus_tag="ACGI8A_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HFH0178272.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20665..21042
                     /gene="spoIIIAD"
                     /locus_tag="ACGI8A_000261"
     CDS             20665..21042
                     /gene="spoIIIAD"
                     /locus_tag="ACGI8A_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HFH0178273.1"
                     /translation="MQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVMFKL
                     NFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEFGGK
                     IIVMSMSFPILLSIVEMVVNIIP"
     gene            21127..22332
                     /gene="spoIIIAE"
                     /locus_tag="ACGI8A_000262"
     CDS             21127..22332
                     /gene="spoIIIAE"
                     /locus_tag="ACGI8A_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HFH0178274.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22392..23009
                     /locus_tag="ACGI8A_000263"
     CDS             22392..23009
                     /locus_tag="ACGI8A_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HFH0178275.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            23021..23575
                     /locus_tag="ACGI8A_000264"
     CDS             23021..23575
                     /locus_tag="ACGI8A_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HFH0178276.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23602..24291
                     /locus_tag="ACGI8A_000265"
     CDS             23602..24291
                     /locus_tag="ACGI8A_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HFH0178277.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNVDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24401..24763
                     /locus_tag="ACGI8A_000266"
     CDS             24401..24763
                     /locus_tag="ACGI8A_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFH0178278.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24848..25354
                     /gene="nusB"
                     /locus_tag="ACGI8A_000267"
     CDS             24848..25354
                     /gene="nusB"
                     /locus_tag="ACGI8A_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFH0178279.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25338..26408
                     /locus_tag="ACGI8A_000268"
     CDS             25338..26408
                     /locus_tag="ACGI8A_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HFH0178280.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26405..27610
                     /gene="xseA"
                     /locus_tag="ACGI8A_000269"
     CDS             26405..27610
                     /gene="xseA"
                     /locus_tag="ACGI8A_000269"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFH0178281.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27612..27827
                     /gene="xseB"
                     /locus_tag="ACGI8A_000270"
     CDS             27612..27827
                     /gene="xseB"
                     /locus_tag="ACGI8A_000270"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFH0178282.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27830..28717
                     /locus_tag="ACGI8A_000271"
     CDS             27830..28717
                     /locus_tag="ACGI8A_000271"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFH0178283.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIGKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28747..29184
                     /locus_tag="ACGI8A_000272"
     CDS             28747..29184
                     /locus_tag="ACGI8A_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HFH0178284.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29218..31083
                     /gene="dxs"
                     /locus_tag="ACGI8A_000273"
     CDS             29218..31083
                     /gene="dxs"
                     /locus_tag="ACGI8A_000273"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFH0178285.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31216..32028
                     /locus_tag="ACGI8A_000274"
     CDS             31216..32028
                     /locus_tag="ACGI8A_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HFH0178286.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32048..33727
                     /gene="recN"
                     /locus_tag="ACGI8A_000275"
     CDS             32048..33727
                     /gene="recN"
                     /locus_tag="ACGI8A_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFH0178287.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33941..34807
                     /locus_tag="ACGI8A_000276"
     CDS             33941..34807
                     /locus_tag="ACGI8A_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178288.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34900..35391)
                     /locus_tag="ACGI8A_000277"
     CDS             complement(34900..35391)
                     /locus_tag="ACGI8A_000277"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0178289.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35452..35892)
                     /locus_tag="ACGI8A_000278"
     CDS             complement(35452..35892)
                     /locus_tag="ACGI8A_000278"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0178290.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36053..37111
                     /locus_tag="ACGI8A_000279"
     CDS             36053..37111
                     /locus_tag="ACGI8A_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HFH0178291.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37253..38077
                     /gene="spo0A"
                     /locus_tag="ACGI8A_000280"
     CDS             37253..38077
                     /gene="spo0A"
                     /locus_tag="ACGI8A_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA];
                     GO_process: GO:0051606 - detection of stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HFH0178292.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38490..39611
                     /gene="steA"
                     /locus_tag="ACGI8A_000281"
     CDS             38490..39611
                     /gene="steA"
                     /locus_tag="ACGI8A_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HFH0178293.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39629..40504
                     /locus_tag="ACGI8A_000282"
     CDS             39629..40504
                     /locus_tag="ACGI8A_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HFH0178294.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40528..41220
                     /locus_tag="ACGI8A_000283"
     CDS             40528..41220
                     /locus_tag="ACGI8A_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0178295.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41228..42052
                     /locus_tag="ACGI8A_000284"
     CDS             41228..42052
                     /locus_tag="ACGI8A_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFH0178296.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKNLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIVFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42057..43124
                     /locus_tag="ACGI8A_000285"
     CDS             42057..43124
                     /locus_tag="ACGI8A_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HFH0178297.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEEFFKAASTAAYYLMEV"
     gene            43126..43662
                     /locus_tag="ACGI8A_000286"
     CDS             43126..43662
                     /locus_tag="ACGI8A_000286"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFH0178298.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43730..44335
                     /locus_tag="ACGI8A_000287"
     CDS             43730..44335
                     /locus_tag="ACGI8A_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HFH0178299.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKSR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44347..45261
                     /locus_tag="ACGI8A_000288"
     CDS             44347..45261
                     /locus_tag="ACGI8A_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0178300.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAAEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45303..46475
                     /locus_tag="ACGI8A_000289"
     CDS             45303..46475
                     /locus_tag="ACGI8A_000289"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFH0178301.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIEGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46522..47334
                     /locus_tag="ACGI8A_000290"
     CDS             46522..47334
                     /locus_tag="ACGI8A_000290"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFH0178302.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47538..48863
                     /locus_tag="ACGI8A_000291"
     CDS             47538..48863
                     /locus_tag="ACGI8A_000291"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFH0178303.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            49076..50155
                     /gene="mltG"
                     /locus_tag="ACGI8A_000292"
     CDS             49076..50155
                     /gene="mltG"
                     /locus_tag="ACGI8A_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFH0178304.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFSYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50327..51001
                     /locus_tag="ACGI8A_000293"
     CDS             50327..51001
                     /locus_tag="ACGI8A_000293"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFH0178305.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRKYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            50988..52235
                     /locus_tag="ACGI8A_000294"
     CDS             50988..52235
                     /locus_tag="ACGI8A_000294"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFH0178306.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEIYEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52307..53977
                     /locus_tag="ACGI8A_000295"
     CDS             52307..53977
                     /locus_tag="ACGI8A_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFH0178307.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDIDKSMKKELEKNGIMVEEKTMRYAKDGL
                     LSHTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKT
                     VDNKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQND
                     ISKSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNT
                     NEILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGL
                     DEEKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNM
                     NTIKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLN
                     KKAIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54074..54715)
                     /gene="sigK"
                     /locus_tag="ACGI8A_000296"
     CDS             complement(54074..54715)
                     /gene="sigK"
                     /locus_tag="ACGI8A_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HFH0178308.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            55282..55644
                     /locus_tag="ACGI8A_000297"
     CDS             55282..55644
                     /locus_tag="ACGI8A_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178309.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            55628..56353
                     /locus_tag="ACGI8A_000298"
     CDS             55628..56353
                     /locus_tag="ACGI8A_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178310.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            56373..56792
                     /locus_tag="ACGI8A_000299"
     CDS             56373..56792
                     /locus_tag="ACGI8A_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178311.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            56782..57306
                     /locus_tag="ACGI8A_000300"
     CDS             56782..57306
                     /locus_tag="ACGI8A_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178312.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            57618..58175
                     /gene="efp"
                     /locus_tag="ACGI8A_000301"
     CDS             57618..58175
                     /gene="efp"
                     /locus_tag="ACGI8A_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFH0178313.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            58267..58722
                     /locus_tag="ACGI8A_000302"
     CDS             58267..58722
                     /locus_tag="ACGI8A_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1247 N-terminal domain-containing protein"
                     /protein_id="HFH0178314.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            58885..59595
                     /gene="rnc"
                     /locus_tag="ACGI8A_000303"
     CDS             58885..59595
                     /gene="rnc"
                     /locus_tag="ACGI8A_000303"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFH0178315.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            59649..60719
                     /locus_tag="ACGI8A_000304"
     CDS             59649..60719
                     /locus_tag="ACGI8A_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongator complex protein 3"
                     /protein_id="HFH0178316.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            60747..64301
                     /gene="smc"
                     /locus_tag="ACGI8A_000305"
     CDS             60747..64301
                     /gene="smc"
                     /locus_tag="ACGI8A_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFH0178317.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            64328..65608
                     /gene="ftsY"
                     /locus_tag="ACGI8A_000306"
     CDS             64328..65608
                     /gene="ftsY"
                     /locus_tag="ACGI8A_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFH0178318.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            65711..66079
                     /gene="ylxM"
                     /locus_tag="ACGI8A_000307"
     CDS             65711..66079
                     /gene="ylxM"
                     /locus_tag="ACGI8A_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HFH0178319.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            66076..67428
                     /gene="ffh"
                     /locus_tag="ACGI8A_000308"
     CDS             66076..67428
                     /gene="ffh"
                     /locus_tag="ACGI8A_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFH0178320.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            67466..67738
                     /gene="rpsP"
                     /locus_tag="ACGI8A_000309"
     CDS             67466..67738
                     /gene="rpsP"
                     /locus_tag="ACGI8A_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFH0178321.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            67803..68030
                     /locus_tag="ACGI8A_000310"
     CDS             67803..68030
                     /locus_tag="ACGI8A_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFH0178322.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            68138..68653
                     /gene="rimM"
                     /locus_tag="ACGI8A_000311"
     CDS             68138..68653
                     /gene="rimM"
                     /locus_tag="ACGI8A_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFH0178323.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            68662..69357
                     /gene="trmD"
                     /locus_tag="ACGI8A_000312"
     CDS             68662..69357
                     /gene="trmD"
                     /locus_tag="ACGI8A_000312"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFH0178324.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            69542..69892
                     /gene="rplS"
                     /locus_tag="ACGI8A_000313"
     CDS             69542..69892
                     /gene="rplS"
                     /locus_tag="ACGI8A_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFH0178325.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            70213..71109
                     /gene="ylqF"
                     /locus_tag="ACGI8A_000314"
     CDS             70213..71109
                     /gene="ylqF"
                     /locus_tag="ACGI8A_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFH0178326.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            71498..72790
                     /gene="brnQ"
                     /locus_tag="ACGI8A_000315"
     CDS             71498..72790
                     /gene="brnQ"
                     /locus_tag="ACGI8A_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFH0178327.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLFVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT        29872 a         7228 c        13577 g        22173 t
ORIGIN      
        1 gtatacaata atatatctat aacaaaataa taaaagatag agttttattt agacatttat
       61 tacaaatcaa aatttattac atattattta taaaaaaatc taaggaggaa ataataatga
      121 aaatattagt attaaactgt gggagttctt cattaaaata tcaattaata gatatgaata
      181 atgaagaagt tttatgtata ggattggttg aaagaatagg aatagaaggt tctatattaa
      241 aacatgaaaa agcaggtaga gatgataaat acgttgttga acaaccaatg aaggaccaca
      301 aagatgcaat agcattagtt ttagaagctg ttgctcatcc agaatttggt gcagttaaag
      361 aaatgaaaga aatagatgca gtaggacata gggttgtaca tgctggagaa aaatttgcaa
      421 cttcagtagt aataactcca gaagtagaag aagctttaaa agaatgtata gatttagctc
      481 cacttcataa tccagcaaac ataatgggaa tagatgcttg taaagctata cttccagatg
      541 taccaatggt aggtgtattt gatactgctt tccatcaaac aatgcctaaa tcttcatact
      601 tatatggttt acctcatgag ctatatacta aatatggggt aagaagatat ggattccacg
      661 gaacttctca taattatgta tctcaaagag cagcagagat attaggaaaa gatataaaag
      721 atttaaaaat agtaacttgt cacttaggaa atggtgcttc tatagctgct gttgatggtg
      781 gaaaatgtgt agatacatct atgggattca ctccattaga aggattaata atgggaacta
      841 gatgtggaga tatagaccca gctatattac cattcttaat gagaaaagaa ggtttagatg
      901 ctgatggatt ggataaatta atgaataaag aatctggagt atatggaatg actggaattt
      961 ctagtgactt cagagatata gaagatgcag ctaaaaatgg agacgaaaga gctcaagcta
     1021 cattagaagc gtatgttaaa aaagtacaaa aatatatagg cgcttatgct gctgaaatga
     1081 atggattgga tgttgtagta tttactgcag gcgttggtga aaatggaaag gctataagag
     1141 cagatattgc ttctaacatg gagtttttag gaatgaaatt agataaagaa gctaatgatg
     1201 ttagaggaaa agaaacagta atatctactg ctgattctaa agtaaaaatg cttttaatac
     1261 caactaatga agaattaatg atagctagag atacattaag attggtaaaa taattaaatt
     1321 aaacctatgc ctatcaagta aaaatacttg acaaacaaat gctagatttg tataataaat
     1381 ttggtgatat gttgaggtga acttaatgaa agttagcata gaaaaaataa atagaaaaga
     1441 aactgacaaa atagacttga atttttgtga aaaaattgat actattagtt attgtgatga
     1501 gatttacaaa ttagtatcac ctgtaaattt aaaagggaaa gtgtcaaaga ctaataaagg
     1561 tttgtatcta gatatagatg tcaattttac aattgttgac aattgttcaa gatgtcttaa
     1621 agaagtagaa ataccactag agtattctat acaaggtttt ttagtaaaag aagaagatta
     1681 tgatgaagat gagtttgagg aatttgatcc ttttatattc gatggtgaag aaatcgacct
     1741 tattgatata atagaacaga cattagattt taatgtacct cataaagttc tttgtagtga
     1801 aaactgtaaa ggtctttgtc aagtatgcgg ggcaaactta aatgaagaag agtgttcttg
     1861 cagtgaaatt acaaacgatg aggaatacat agatcctcgt tttgctaaat taaaagattt
     1921 atttaattaa attttgacta aggaggtggc gtaaatggca gtaccaaagc gtaaaacgtc
     1981 taaatcaaac acaaaaatga gaagagcagc taactcaaag atggaagcaa caggatttgt
     2041 aagttgccca caatgtcatg agccaaaatt accacataga gtgtgtccag actgtggata
     2101 ttataaaggt aaagaagttg tgtctaagta atagcttcta aaaagcatgt agagtatact
     2161 ctacatgttt tttttgtaca tataaaaaaa taagataatt tactagagaa aataagtgga
     2221 ttttatgcat attaaaaaat agaaaaataa taatataagt attgaaaatt tttgtgtgtt
     2281 actatatact actattagta gctggtacta attacagcta taaaatttgg agggaacatg
     2341 aaaaagaaaa gtaaagccca aagacaaaaa gagcttatag atatgctaaa gacagatcct
     2401 ttttatactg atgaagaatt atcaagtctg tttgatgtaa gtatacagac cataagatta
     2461 gatagaatga gtctaaacat accagagcta agagaaagag tcaaatcaat agcagaaact
     2521 cagagttcaa aagtaaagac tttaggagta aaggaaatta caggagaaat cattgattta
     2581 tctgtagggc gattaggtat atctatgtta gaagttacac aggacatgat ttactctaag
     2641 actaatactt taaaagatac gtatattttt tctctagcag attcattggc aatggctata
     2701 atagatgcac ctaaggttat tatgagagtt gcaaatgtaa aaagttttaa gttgatagaa
     2761 caacaagata ggcttatagc aaaagcagaa gtgtatagaa atatagataa aaagcattat
     2821 gtaaaagttg ttatcaacaa taaagcccaa gagcaaatat ttagaggaaa gtttatcttt
     2881 gaagaattag attaggaggg aaattatgaa aatagtaatt gatggaatgg gtggagataa
     2941 tgcacctaaa tctaatgtag aaggcgcagt aaatgctata aaagaatatc aggttgattt
     3001 aataatcaca ggagataaag atttacttga aaaggaattt tcaaattatg aatttgatag
     3061 aaataaatta gagatagttc atactactga gattatagaa aacgaagata aaccagtaaa
     3121 ggctataaga agtaaaaaag attcatctat ggtagtagca ttaaatcttg taaaagaagg
     3181 aaaggcagat gctataatat cagctggaaa tacaggagcc ttacttgctg gtggattatt
     3241 tgttgttggt agaataaaag gaatcgatag accttgctta tgttcagcaa taccaaatgt
     3301 aaagcgagga atgacactta tagcagactg tggcgcaaat gcagattgta aacctaagaa
     3361 tctagttgaa tttgcagcta tgagtaatat ttattcaaga aaagttttag gtcttgaaaa
     3421 tcctaaggta gcattagcaa atgttggact agaagaaggc aaaggcaatg atttagtcaa
     3481 aaggtcatat gaagaaatta aaaaattaga tttaaatttt atagggaatg tagaagctag
     3541 agaagttata aatgcataca cagatataat catatgtgat ggatttacag gtaatatatt
     3601 acttaaatct gctgaaggtg tagcactttc agttatgagt cttataaagg aaacttttat
     3661 ggccagtaca aaaagtaaaa taggagcatt acttataaag gatgatttaa gaaaattaaa
     3721 aagttttata gattattctg agtatggggg agctccactt ttaggtctaa atggaggagt
     3781 tataaaagct catggtagtt ctgatgctaa agctataaaa aatgctataa atcaaggaat
     3841 aaaattttca aagggaaaag tcgttgaaga tattaaccaa tttataagca aatacaatga
     3901 agaaaataaa aataacgaag atgaataaac tcgttttgga ggtttaatat ataatgaata
     3961 caaaggctgg aatattagga gtaggaagtt atctacctga gcaatcatat gataattttc
     4021 acttcgaaaa aataatggat acatcagatg agtggataag tactagaact ggtattaagg
     4081 aaagaagatt tgccaaagag tctgaagcaa cttctgactt agcatctaaa gctgccttaa
     4141 aagctataga atgtgcaaag ttaaacgtag aggatataga gctgattata ttagctacaa
     4201 ttactcctga tatgtctttg ccttcaactg catgtatagt acaagatgca ataggagcag
     4261 taaatgctac tgcatttgat atatcagcag cttgttctgg atttgtatat ggagtaacta
     4321 ttgcaaagca atttgtggaa acaggttgct ataaaaatgt gcttgtaata ggagcagaaa
     4381 cttgttctaa gtttttaaat tatgatgata gaacaacagc agtattgttt ggagatggag
     4441 ctggagcagc tgttattggc cctgtaaatg agggtggaat actgtctaca catatgggct
     4501 ctgatggtaa aggaaaagat tgtttaaaag tacctgctgg aggttcaaga cttaaggcaa
     4561 gtaaagagac tgtagaagct aatctacata caatagaaat ggcaggtagt gatgtgttta
     4621 aatttgcagt tagaaagatg gcagaaactt ctttaagagc tcttgaaaaa gcaaatctaa
     4681 atacaactga tatagactac ttagtgccac atcaagctaa tataagaatc atacaagctt
     4741 cttctaagag attagagctt gatatgaaaa aagtttatgt aaatatagac aaatatggaa
     4801 atatgtctgc tgcatctata ccagtagcat tagatgaagc ttatagagaa ggtaaaatta
     4861 aaaagggtga taatgtagtt ttagttggct ttggtggagg tctaacttgg ggggcttcag
     4921 ttgtcaagtg gactttatag tataaaatat ctaaaatgat ttaattaaca aaattaaact
     4981 tatacacaaa ataaagttga ttaataataa aaagtttact tttatgattt caagtgaagg
     5041 agaattgtat gaataaaatt tgcaaaatat taaatataaa atacccagtt atccaagggg
     5101 gaatggcatg ggtagctact gcatcattag caagtgctgt atctaatgca ggaggacttg
     5161 gcataatagc agcaggaaat gcaccaaaag aagctataaa gaaagaaatt gttgagtgta
     5221 aaaaattaac agataagcct tttggagtaa atgtaatgct tatgtcgcca tttgttgatg
     5281 atataattga tttgattata gaagaaaaag ttcaagttat tactactggt gctggaaatc
     5341 ctgcaaagta tatggataga ttaaaggaag ctggaacaaa ggttattcct gtagtaccta
     5401 caatagcttt ggcacaaaga atggaaaagc taggagctac agcagtaata gcagaaggta
     5461 ctgaaggtgg aggacatata ggagaactta ctactatggt cttagttcca caagttgctg
     5521 atgctgtaaa catacctgta atagctgctg gaggaattgt agatggtaga ggaattgcag
     5581 catcatttgc attaggtgcc agtgcagttc aagtaggaac tagatttatt tgcagtgaag
     5641 agtgttctgt ccattcaaac tataaaaact tagtactaaa agcaaaagat agagatgcaa
     5701 ttgtaacagg aagaagtact ggtcatccag taagaacatt aaaaaataaa ctatcaaaag
     5761 aatttttaaa gatggaacaa aatggagcta ctcctgaaga actggataaa aaaggtacag
     5821 gagctttaag atttgcaaca gtagatggag acatagaaaa aggttcattt atggcaggtc
     5881 aaagtgctgc tatggtaaaa gaaataacac cttgtaagga aattatagag gctatggtaa
     5941 atcaagcaag agagattatg ccagcaatag aactgtaaat caggaggagc aagatgggaa
     6001 aagttgcact agtattccct ggtcaaggag ctcaatatgt aggtatggcc aaggatttat
     6061 atgaaaataa tgctgttgct aaaagtgtaa tagatgaggc tagtgatgcc ttaaatatgg
     6121 atttaaaaaa ccttatgttt aatggaaatg aagaagaact ttctaaaact gaaaacactc
     6181 aacctgctat ggttacacat agtgtagcag tattaaaagc ggtacaagct caaatagatt
     6241 taaaatataa tgcatgctta ggattatcct taggagagta tagtgcgttg gttgcagcag
     6301 atgcaattga ctttaaagat gctgtatgtt tagttaaaaa aagaggtaag tttatgcagg
     6361 aaacagttcc agcaggtgtt ggagctatgg cagcagtttt aggtttagat agaaatgtac
     6421 ttgaaaatgt ggttaaagat atacaaggag gtattgtaga agtagcaaat tacaattctc
     6481 ctggacaaat agttatatct ggtgaaaatg aaaagataga agaagcaatg gtaaaatgca
     6541 aagaagcagg agcaaaaagg gcagttaaat taaatgtaag tggacctttt cactcatcaa
     6601 tgttaaaaga agctggtgta aaattggcca gtgaattaga aaaagttaac ataactaaac
     6661 ctaaagttga tgtggtggca aatgtaaatg cagattacta taaaaacgaa ggtaaagacc
     6721 tgcttataaa acaagtaagt tcatctgttc tttgggaaga ttctatagaa agacttttaa
     6781 atgatggata tgatactttt atagaaatgg gacctggaaa aactttaaaa gcatttatta
     6841 agaaaatagc ttccaataaa aaagcaaatg tcaatattta taatatagat ggtatagatt
     6901 ctcttaatga atttgtacaa aattatagaa atggagagat ataatgataa atcttacagg
     6961 acaagtagca gttgttactg gtggttctag gggtatagga aaagaaatag caaaaaaact
     7021 agcatctttt ggagctgatg tagtaatcaa ttatacttct aaagaagatg aagcactaaa
     7081 aactaaaaat gaaatagaaa gtatgggggt aaagtgtacc tctataaaat gtgatgtgtc
     7141 taaatttgat gaagtgaatc aaatgataga ttctgttgta agcgaatttg gaaaaattga
     7201 tatattggtt aataatgcag gcataactaa agatggtctg cttatgagaa tgaaagaaga
     7261 agattttgat agagttatag atataaactt aaaaggtgtc tttaattgta caaaagcagt
     7321 tactaaacct atgatgaaaa agaagtatgg aagaataata aatatgactt cagtagttgg
     7381 aattatgggt aatgcagggc aaactaatta ttgtgcatca aaagcaggtg taattggatt
     7441 tacaaaagct tctgcaagag agttagcatc aagaaacata aatataaatg cagtagcacc
     7501 tggatttata gaaacagata tgactaaagt actaagtgat gatgtaaaag aatcaacact
     7561 agcaaacata ccaaagaaat cttatggtaa accagaagat gtagccaatg ccgtagcatt
     7621 tttagttagt gacatgtcaa gttatataac aggacaagta ataaatgtag atggtggaat
     7681 ggtaatgcaa taataattta aaaagtagag aaatctatta aaataaaaaa ttttaggagg
     7741 tacacaatgt ttaataaagt tgtagaaata ataatagagc aattaggagt agaagataaa
     7801 gaaataacta tggaaacatc attaatgaag gatttagaag cagattcatt agatgcagta
     7861 gaaataataa tggcacttga agatgaattt ggtatagaaa taccagatac agaagcagaa
     7921 aacttcaaat ctataggaga tatagttaag tatatagaag ctaataaata gtcgagcttt
     7981 aaaaaagtgg aggtatccga tgaataaaag agtagtaata acaggacttg gatgtgttac
     8041 gccgttggga acaggaaaag aagagttctg gagtaatata aaatctggag tatctggtat
     8101 agataaaata actaattttg atgctagcac atatcaaact cagatagcag gagaagttaa
     8161 gaattttcat ccagaagaat atataagtaa aaaggaattg aagagattag ataaatttgc
     8221 tcaatttgca atagtatctg ctaaattagc agttgaagat gcaaatcttg atttagataa
     8281 agttgataga gaacgttttg gtgttataat aggttctgga ataggtggag ttgaagctat
     8341 agaaactcaa cataaaattc ttttggaaaa aggaaataaa agagtaagtt cactttttgt
     8401 acctatgatg ataggaaata tggcagctgg tcaagtttca atatttttag gagcaaaagg
     8461 acctaacact aatgtatgta cagcttgtgc atcaggaact cattcaatag gtgatgcttt
     8521 taaggtaatt caaagaggtg atgcagacct tatggtagca gggggttctg aagctgctgt
     8581 aacaggtctt gcttttgctg gattctgtaa catgaaggct atgtcaacta gaaatgatga
     8641 ccctaaaact gcatcaagac catttgataa agacagagat ggttttgtaa tgggagaagg
     8701 agcaggtatt gttatcttag aagacttaga acatgcttta gcaagaggtg ctaaaatata
     8761 tgcagaagta gttggatatg gtttaactgc tgatgcatac catatgacaa ctccagctga
     8821 aaatggagag ggagcagcta ggtctatgaa tatggctttg aaagatggaa atgttccatt
     8881 agaagaagta gattatataa atgctcatgg aacttcaact tattacaatg atttatatga
     8941 aactatggct ataaaaactg tatttggaga aaaggcatat gacttatgtg tgtcatcaac
     9001 aaaatctatg actggtcact tattaggagc atcaggtgct atagaggctg ttgtttgtgc
     9061 tatgagtata gaagatagtt ttgtgcctcc aactataaac atacaagaag ttggtgaaga
     9121 tttggattta gattatgttc caaatcaagg taaagaaaaa aatattagat atgcactatc
     9181 aaattcttta ggatttggtg ggcataatgc tacaatagta ctaaaaaaat atgtataaat
     9241 aaatattaaa tataaaacta aaaaacaagc acttgtttta actgtataaa tttatattag
     9301 cttaaattta aagtctccta aattatttta ggagattttt ttattaagat ttaagtctag
     9361 gatatgttgt aaatcatttt gatatattgt attattaatt attaagaggt tatttatata
     9421 tgcaagtttt cattctacaa tatacaaaag gaggactaaa attttgaatt ttagactacc
     9481 aaagaaaagg ttacccaaaa agataatttt tatatgtata atacttatta gtattatttt
     9541 gtttataaag tatgaccaaa atggattgaa agaaagagaa attataaata agggaaaata
     9601 tatgtctgaa agccttgtgg cagataagga gtatgatgaa gcaaaaaaag tggttaagac
     9661 ttcttttgaa tctattccag aaaaaaaata caataaagta tataatgaag tttgggatat
     9721 gttaaagact atttcatatg ttccagatgg aacgaagata gtcattgatt ctttagaaaa
     9781 gatgagagaa tcagatgtga aattatctga tgaatgtaga tttaatatag aaaaaagatt
     9841 agcatcagtg tatataatgg ataataatta ttctaaggca gtagatttaa ctgtaaagtc
     9901 tataaagttg gctgagaaat tggggaataa ttatgaaaaa gcaagactag atgttgattt
     9961 ggcgagcata tttttaaaga taggtggcta tgaaacagga gagaaattaa taaaggattc
    10021 ttttaaaata gatattgatg aaggtgaaaa aaatggaatg gttagattat atgctattat
    10081 aaatctagca gaaatatatg tagaagaggg cgaatatgat aaagctattg aaataagtag
    10141 caggataaaa gattataaaa agtttgtaaa tacggataat tacaatgatt ttgatataat
    10201 ggcctctata atgcagtcaa atgcgtatgt gggcaaaaat aatatacaaa aggcaaagac
    10261 ttttttagaa aaagctgatt atcttataaa atcagataga acagtttata tattagataa
    10321 agatatgtac tattatatgg caatgggaaa cttagaatat aaaagcgaaa attataattc
    10381 tgcaatagat aattataaaa aaagtttaga gatatctact aaaagaaagt taacccagga
    10441 gaaaataaaa aatttaaata agatattaaa tatatatgaa aaacaaggaa atatagaatc
    10501 tttaaatgag tatcaaaaga tgttaataaa tgaatataaa gagaacaaaa gtataagaga
    10561 ttcagagact agtttctata taattgataa agtatcaaac gaaagtaagc tatttgaaaa
    10621 aactaaaaaa gaaataaaat attataaaat attatttaca gtaattttaa ttgctatatt
    10681 agggtctact tttttataca accgattgaa gtattttaaa gcacaaaatt tacatgatgg
    10741 tcttacaaat gtttataata gaaaaagttt tgatattatg tatgaaaagt acagagagaa
    10801 agatgataat tttgcacttg tgatgataga tattgataat ttcaagctca taaatgataa
    10861 ttatggacat aaatttggtg acactgttat aaagggaata acaaatacca tttgtgttat
    10921 gttagaacaa gatgataaag tctttaggta cggaggagaa gaattttctg tattaataag
    10981 aaataaatct ggtgatgaag tagcagatat aattgacagt ataagggttg caattgcaaa
    11041 taaaaagtgg aatgatgatg tgactgtgac tataagtgca ggtgtagctc atgtttcaga
    11101 tagcaaagat gtactagaag aagctgataa aaatttatat aaagcaaaac aacttggtag
    11161 aaataaagta gtatataagt aattatatac tatataaata agaggtataa atatgaaaca
    11221 atcaatagct atagtaactg ataaaataac taaactatca tctttcttag aagaaaatat
    11281 acgtttggtt ttaggtaatt atattgatat aaaccactat tatctggaag ttttaaaaga
    11341 gaatgataaa atacaaggtg attttgtact agtaatgaat gatgatagat taaacagtat
    11401 gagaaagcat ttagtacaag atagtaagat tatagtgata aggagaacac ttaaagaaaa
    11461 tgaaatatat ggattatttt caataccaaa tggaactgaa gtattggttg taaatgatac
    11521 aaaagaaact accttagaaa cgataagctt attttacaaa ataggagtaa aaaatttaaa
    11581 gtttactcca tatattgaag gaaaaattta taaaaatata gacatagcta taacacctgg
    11641 agtaaaggga aaagtaccaa gttatataaa taaagtaatc gaccttggaa atagatatat
    11701 tgatatatct acctttatag aaataataaa tggtcttaga atagatgtta aagaaatacg
    11761 taaaaattta atggcatatt ctgagagttt aataagttta gataatggag ttaaagataa
    11821 ttataggcaa ctgtttttaa aaatagaaga acaagatgtt atattgaatc tatcaaaaga
    11881 tggaataata tttacttcag cagatggtat aataaacaca tttaataaag aagctttaaa
    11941 aattttaggt ataaatgaaa atccacaagg caaaaacata gaagatatca tttctgaaga
    12001 tttaaatatt ttacttgaag acaaagaaat aattgatgaa gtggtattta caaataaaaa
    12061 atatataaat gtaaataaaa aaagtatttt ttctatgggg aataaagctg ggatatatta
    12121 taacttacaa gaaataacgt atataaaaaa attagaacaa aatcttacta ataaattaag
    12181 agaacaaggt cagatagcaa gatatacatt taaagatata aaaactaaaa ataaaaatat
    12241 ggtaaagtgt gtagaattgg ctaaaaaaat atctaaatca gacctttctg tattaataat
    12301 aggagaaagt ggtactggta aagaattatt atcacaatca attcataatg cttccaatag
    12361 aagtaatcaa ccgtttatag cagttaattg tgcagcagtt ccagatagtc tattagaaag
    12421 tcaactgttt ggatatgaaa aaggctcttt tacaggagct ttaagagaag gaaaaaaggg
    12481 tctatttgaa cttgctaata atggaactat atttctggat gaaattggag atatgcctcc
    12541 attgctacag acaaagttac taagggtatt acaagaaaag caggttatgc ctataggttc
    12601 ccataatgtt ataaacatag atgttagggt catagcagca acaaacaaaa acttactaaa
    12661 gatgattaga gaaggaaaat ttagagagga cctgtattat agactaaatg tattgccaat
    12721 agatgtacca tcattgagga atagaaaaga agatataata acacttatga atttcttttt
    12781 gaaaaacaat ataagacttt cgccagaagt tataactatt ttagaaaaat ataattggcc
    12841 aggaaatata agagagttac aaaatgttgc ttcttatgta agtctaatgt gtgatagtat
    12901 ggtttttaga gatgacttac caccatatat aagggtagaa tgtgctaaca attatgaaga
    12961 tggatttggt tatacaagaa aagaatttga ggaaatactg aggattctat atgaaagaag
    13021 agagtcatcc tctggaattg gaagagggtt tataatcgaa aagcttatac aggaaggtat
    13081 tgagatttct gaaagcaaag ttagaaaggt gctatcttat ttaagtgaat gtggatacat
    13141 atcatctaaa tctggtagag gaggaagtca aataacctca gaggggataa aactttataa
    13201 taatataaat taagctaagt atttgatatt gaaatcacat attttgaatt tagatattta
    13261 aataataata aacttttata tttatagaga ttaatttata ttatgtcaga aagaatattg
    13321 taatttataa ttaaatatta ataggttttt aaaataggat tagataggta ataggttaag
    13381 ttagaataac ccattaccta tttttatttg taagaaaagt gtacatagaa ttagaagtaa
    13441 aaaatacaac taataaaact attgaaaata ctagataaat aataaataaa aataattata
    13501 gattttggca tacatattgc taattataga gataagacga caaaataatt gggaggttta
    13561 atgatgaaat acaaaacaac agggacttgt gctacagaaa ttgaatttga agtaaaagaa
    13621 aataaagtta caaatgtaaa ctttataggt ggatgtgatg gaaatttaaa agggctaaaa
    13681 gtattagttg aaggcatgaa tatagaagat gtaatacaaa agttaaaagg aatagagtgt
    13741 aaaacaaagc caacatcttg cccagaccaa ctgtctctag cattggaaaa ttatatgaat
    13801 ataaagtaat acataaaaca atcgattagt taggagttta attatgaaac caataatagg
    13861 gattttagga aatttaataa taatggaaaa tggtatgttt ccaggtcttg aaaggtcata
    13921 tgtaaataat gattatataa atgcagtgct aaaaggtggc ggtagtccag taataattcc
    13981 tgtaaataca gataaagaag ttataaaaaa acagattgaa atggttgatg gagtcttaat
    14041 atctggggga tgggatatca atcctcaact gtatggagaa gaaacaagag aagaaacgac
    14101 atttatatat ccagaagtgg atgaatttga tttaattgca ataagtatag cattagaact
    14161 taaaaagcct atattaggtg tatgtagggg actacaaatt ttaaatgtaa gtttaggagg
    14221 aactttatat caagataata atcttataga aggaagttat ataaaacata cacaatcttc
    14281 taaaagacat gtagctactc ataaagttga tgttaaagaa ggaagtattt tagagggaat
    14341 tttaggaaaa caattactta caaatagtta tcatcatcaa agtgtcaatc agcttggaaa
    14401 aggattaaaa gccatagcat actcaaaaga tggtataata gaagctattg agaaagagga
    14461 tgaaaatttt gtagtaggag tacagtggca tccagaaatg atggtagatt actgtgataa
    14521 aatggaaaaa ctatttaaat attttataaa tatttgttcg aatttataat ataccttggg
    14581 gggattgaac atgaacgaaa agaacaaaat gggattaata agtattatac ttttaggtat
    14641 taatgcagta gtaggagctg gagtttttct attaccagga gatgcaatga aatcatttgg
    14701 tgtagcaagt atatttgtat atatatttga tatgttgtta gttttatcta tggcattctg
    14761 ttttgctgag gttgcaggta aattcaataa aaatggtgct gcctatgtat atacaaaaga
    14821 agcttttgga gatttttgtg gttttgaagt aggtcttatg aaatgggtaa taggatgtat
    14881 ttcttgggga gctttaatag taggttttcc tacatcatta tcagcagtat gggctccagc
    14941 aggtgaacca catatacaaa aaattataat tgtagctatg atagttgggc ttacaattat
    15001 aaacttatta ggtgtatctc tatcaaaaat tgtacaaaat gttataactg taggtaaact
    15061 aattccatta atactattta taggaatagg tatatttttt ataaaaggag ttaattttac
    15121 ttcttcaaca atggtacctc caggagctgg agcaacagag tttggagcag cagcactttt
    15181 aatgttttat tcatttacag gatttgaatc tatagcagtt gcagcagaag atatggaaaa
    15241 tccacaaaaa aatataccaa tagcaattat aagtgtaata gtaattgcat caataatata
    15301 tatattaaat caagttgtat gtgttggtat actaggagat tcgttatcta gtacatctac
    15361 accagtggca gatgctgcaa gaatatgctt tggaaatatg ggagctggat tggttacctt
    15421 tggaacattg gtttctgttg gtggaatttg tatgtgtggt gcttttgtaa atccaagaag
    15481 ttgtgtagct ttagctgatg ataaaatgtt accgagaata tttgcaagaa aagataaaaa
    15541 aggtacacca tatgtagcga taatagctac tatgttaatc actataccaa tagctctatc
    15601 tggaagtttt gctgaattag cagcaataag tgcagttgct agattcatac aatacatacc
    15661 aacatcttta tccgtattag tgtttagaaa gaaaagacca gaattagtag gtacttttaa
    15721 aactcctttt ggagcagtga ttcctctaat tgctgtatgt gttggaacat ggttgttatt
    15781 ccaagcatct atgcatcaat taattatggg attaggagct ttggcaattg gagtaccatt
    15841 atactttata atgaagtctt ataatagaaa aatctatggt gaaaatttaa agaaaatagt
    15901 ttaattatgt tataacaatt tattgagtta aaattagaca ttggcagaaa attaaataca
    15961 aataagtatc tacaaggcta tattaaaata ttattaattt tttattttaa tatagcctta
    16021 tattgttatc aataaatgaa aaatattgta acaacagaag aacaatagta ttatgtttat
    16081 agacaaaaaa cttattagga ggtaaattta tgccaacaat aacattaaaa aatggtgtag
    16141 acgtacttat tagagaaggt gtaagagaag atgctcaaag cattatagat ttttataatg
    16201 aagtgggtgg agaaactcat tttctgtcat ttggaaagga tgaatataaa atatctttgg
    16261 aggaacaaga aaatgctata gaatcagcaa aagcatctga taattctgtt aaactgattg
    16321 catttataga tggtgaaata gttgggattg caacgattga ttctaatcaa aaagcaaaag
    16381 gaaagcatgt tggagtatta ggaattgttg taaaagaaaa atattgggga attggattgg
    16441 gtaaaaggct tatgttagac cttatagaat ggtgtaaatc aaatggaata accaagaaga
    16501 taactcttgt cactaatgaa gaaaattata atgctatagg attatacaaa aaagttggat
    16561 ttgaagtaga aagcatacta aagaaagaat gttattataa tggtgtttat actgatttaa
    16621 taggaatgtc acttttattg ggaatataat tagtgtacat tttatctcga aaaaagtata
    16681 ttaagtatgg tataattaaa aaagaatata acttaatata attttggagg acgaattatg
    16741 aaaaatctgt gtaaaatatc agatgattat ttaaacagca agcttaagta tttaaagtta
    16801 ttatcaaaac aatatccaag tatatcaaaa gcaagtacag aaataataaa tttagaggct
    16861 atattaaatc ttccaaaggg aacagagcat tttataacag atgtacatgg tgaatatgaa
    16921 ccatttgtac atgtattaaa aaatggttct ggtgtaataa agagaaaaat agaagaattg
    16981 ttttcaaata caataagaga cagcgaaaaa aaaatgttag caactcttgt atattatcct
    17041 gaacaaaagt tagacctaat aattaagcaa gaggaaaata tagatgattt ttatagaata
    17101 aatatttata gacttataga actttgtaaa tatgcttcta gcaaatatac taggtctaaa
    17161 gttagaaaac tgttaccaga aaattttaaa tacataatag aagaactttt gcatgagcat
    17221 gttaaaagtg aacataaaga agagtattat aaaagtatag ttgaaacaat agttgacata
    17281 ggaatagcta aggagtttat aattgctatt tctacagtta tacaaaaatt ggttgtagat
    17341 agacttcatg taataggaga tatttatgat agaggtccaa gaccagatat aattgtcgat
    17401 aagcttattg aacatcattg tgtagatatt caatggggaa atcatgatat attgtggatg
    17461 ggtgcagcct caggtgaaaa aacttgtata gcaaatgcac tgaggatatc agctagatat
    17521 gcgaatttag acattgtaga agacatatat ggaataaatc tattgccttt agccactttt
    17581 gctatagaaa tgtataaaga tgacccttgt aaagaattta ttccaaaggt taatgaccaa
    17641 agtgtaacaa caacagaaaa gtctttaatg gcaaaaatgc acaaagcaat aagtataatt
    17701 caatttaagc ttgaaggtga ggttatcaga cgaagacctg aatttgaaat ggaacataga
    17761 ctcttgctta atatgataaa ctatgatgaa ggtacgatta atttaaaagg taaaacatat
    17821 aaattaaaag atacttattt acctacaata gacaaaaaag acccatataa attaactatg
    17881 gaagaaagaa atgttattga taaattagta tcttcattta gaggtagtga aaaactacaa
    17941 aaacatgttt catttttgtt ttctaaagga agtatatatt taaaggctaa ctcaaattta
    18001 ctgattcatg gatgtgtacc gttaaatgaa gatggaagct ttatgtctat gaatataatg
    18061 ggaaaagagt ataaaggtaa ggctcttatg gataagatgg agtctctagc aagagaagga
    18121 tttttcttta aagataaggc agaagaaaaa ctgtatggca tggatattat gtggtattta
    18181 tggactggga aatgttcatc attatttggt aaagatgata tgactacatt tgaaagatac
    18241 tttatagctg aaaaagagac tcataaagaa aataagaacc catattttaa acttagagaa
    18301 aatgagatgg cttgtaaaag attatttgaa gaatttgatt tagaacttga tgaatctcat
    18361 ataataaatg gtcatgttcc tgtggagagt aaaaatggag agagcccaat aaaagctaat
    18421 ggtaaaattc tcgttataga tggtggtttt tctagggctt accaaaaaac tactggtata
    18481 gctggataca ctttgatata caattctaga actttgcaat tagtatcgca tgaaccattt
    18541 aattctgctg aagaagccat tgcaaatgaa agtgatatct tatcgacaac tgttgtagta
    18601 gagcataagg caaagagaaa aatggtgaga gatactgatg aaggaataaa gatacaagag
    18661 gaaatagaag atttaaaact acttttaatg gcttataaga aagggctaat aaaagaaatg
    18721 tagatgttat agaggcactt catgattgaa gtgtcttttt gttaatactt attaaatata
    18781 ttataaagat taataatagt agtttatatt atacttacta agtgaatcca agataaatgg
    18841 gttcactttt tttatttata aacattatta aaaaaatata gatgttttgg ttatattcct
    18901 tgtcctttat aaatagaatt atataaacaa aggaggagga caagagatga ataaactttc
    18961 tgatgaaata ataaactctc tttctacaac tataagagaa aaggtagaaa aagtctcaaa
    19021 caataatctg aacatagaag aaattaggtt acgctcacaa aaaccattga tactaaatgc
    19081 aaactctaaa gattattttt acaatcaaaa aactatgaca cttgatttaa atcaacaaaa
    19141 ctcatatgta gtaacaagag aagatgtaga gcaaactttt caaataatat gtaaatattc
    19201 aatacattca tttatggatg acataaaaaa aggattcata actttgagag gaggtcatag
    19261 agtagggtta gttggaaaag caatagtaga agatggacaa gtcaaaaata taaagcatat
    19321 atcatcttta aatataaggg tatcaaggga aattattgga tgctcggata agattttaag
    19381 tcatatcata aagggaaaaa atcagattaa taatactttg ataatatcac cacctcaatg
    19441 tggaaaaaca actttaataa gagatatagt aagaaattta agtaatggta atgaagatta
    19501 tggatttaaa ggtttaaaag tggctttagt agatgagcgt aatgagatag caggagctta
    19561 tctaggtgtt ccacaaatgg atgtgggaat aaggactgat ataatagaaa cttgcccaaa
    19621 agacttgggt attacaatgc ttttaaggtc tatgtcacca aatgttattg tgacagatga
    19681 aattggaagt gaaaaagaaa taaaggcatt atacacagca ttaaatggag gtataggatt
    19741 aataactact gtgcatggag attcaataga ggatatacaa aatagaaaag aattaaatag
    19801 acttctggat aaagagttat ttaaaaaggt tataattctt tctgcaaaaa gaggagcagg
    19861 aacaattgaa aaaatttatg atttagaaga aaagagatgg tattttgcaa attaaaataa
    19921 ttattatagc tttcttgata ggaagtagtt atttgatagg tgaacaaata tacaaaactt
    19981 atactcgtag acataaacaa ttaaacgatt taataagagt tttagaaatt ttgaggatgg
    20041 atttatcttt tggactttat acattagaag aaatctttaa taggataggt ggaaataaag
    20101 agttttgttt ttggaaattt ttctatcaaa tatcagaagg acttcacaat gaacaaagta
    20161 aaactttgga aataataata tcagaaaata ttgatgtctt aagtaaagaa acttatctag
    20221 gcaataaaga aattgaggaa ctgaaaaatc taatacttac tttagggaaa agcgatatag
    20281 agtcacaaca aagaatgata gatttatcta tagaaaattt gaaaaaacaa acctatgaga
    20341 caaaggaaga tataaacaaa aaaggcgtat tgtataaaaa attagtgaca ttcataggta
    20401 taggtatatg tattatattg atttaatatg gggaggttta ttatggaaat atcattgata
    20461 ttaaaggttg caggtgtcgg tattctaata tcagtattga atatgatatt ggaaaaaaca
    20521 gatagaaaag attgggcagg tcttactaca ttagcaggtg taataatcgt attgggtatg
    20581 gtaataacag aaataagtga cttgtttaat actgtaagaa caatgtttca actttactaa
    20641 agaggaggga gtaatttgga aataatgcaa ttaataggaa ttgcaatcat ttcaactaca
    20701 ctctgtttag taattaaaaa agatagacca gagatagcaa atttcatagc tataataact
    20761 ggagtaataa tactattgtc agttatgttt aagctaaatt ttatagtaga tagtatacaa
    20821 gatttggcta acaaggctaa catcccaact atgtatatat cattaataat aaaattaatt
    20881 gggatagcat acttaatgga atttgcgata caactttgta aagattgtgg ggaaggaaac
    20941 attgcttcaa aactagaatt tggaggtaaa ataatagtta tgtcaatgtc tttccctata
    21001 cttttgtcca ttgtagaaat ggtggtgaat ataattccat agatgtgtat cttggaatga
    21061 atataagtat attagagagc tttcgttgta ttgaggttta tagagtgaag agaggcatgg
    21121 ttgcttatga aaaaaagatt tttaccaatg attataggtt ttttatttac tttctttttt
    21181 gtaaatatct ttgctattat gatatttgct aatgaagtac caagtagtga agataaagaa
    21241 tatggtgaaa caaaaaatag catagataag tatatagatg gtcaattaga caagctagat
    21301 ataaatgaga ttcaagacta tataaataaa gaaatagtta ttaatgatgt taatttaaaa
    21361 tcatttgtaa aagatttaat tagtggagaa aaaaatatct tagatttatt caataaagat
    21421 ggattgaaga tattgatgtt tgatgaattt aaagcaagtt taaaggtagt agcagtaatt
    21481 ttggtactgg ctctgttatc atctatttta aagagtctgg aaaactcttt ttcatcaggt
    21541 gcagtaagtc aaatagcaac atatataata tttatcacaa tggtatcact tacacttatt
    21601 ggttttaaag atgtattaca gatttgctat gatgcaatag accatactgt aggtcttatg
    21661 caagttataa tgccaatatt aataacattt ttacttttaa ttggattccc aataacgtcg
    21721 actactttga atcctatctt cataggagga gtaacattta taaatgtatt ttttaaaaat
    21781 tttctatttg tatctattac tgttgcattt ggaattttaa taataaacaa tttatcaaaa
    21841 aatataaggc taaagagatt tttctccttt gtaaaacaaa taaattatgt ttctattgga
    21901 gctatgttta ctgtttactt aggtcttgta tccatacaag gattgtatgt aaccagtttt
    21961 gataaattca gtgtaaagac tgctaaattt gctataggta attttattcc tgttgtaggt
    22021 ggatttgtat ctgactctgt ggatatttta ttatcttcat ctcagcttat aaaaaatata
    22081 tttggaggaa taggacttat cttacttgtt ggaatttgct tacttccagt aataaaaatt
    22141 ttatcagtaa tagtagtgta caaattagca gcaataattg tagagcctgt tggagaggat
    22201 ggaatatcta attttttaaa tgaagtggca aatttaatga taataatgct agcatcagta
    22261 attgctataa cagtaatgtt ctttgtaact gtagctatct tgacctcgat aagtgtcgta
    22321 agtcaagggt aatttatata tcagtgaaaa ataattacat ttaatctaat gtttttagag
    22381 aggaggatgc tatgttagag ggcataaagg catggatagt tagtgttttg ataggagcat
    22441 ttatagtaaa tatagtagat atgattttac caagttctaa gataaaacct tatgtcaacc
    22501 tagttttaaa ctttatgttt gtatttattg taataactcc agtagtagga tttttttcaa
    22561 aagatatgag tttagaagat agaatattaa aatctatggg aaactacaac aagcagtatg
    22621 tagatagtac aaatgctttg gcaaaagaga ctggaaataa tagtctatcc aagggatatg
    22681 aggatgggtt aaaagaagta cttaaattaa aacttgatga atatggttat gatttagaag
    22741 atatagagct taatggggca aatataaata atataaaaat aaaagaaaag aataatagtg
    22801 caaaaagtaa taacagttcc aatgaagaaa ataaaaataa tagtactaaa agagaggaag
    22861 aaaacataaa ctctaatgac aaagaaaatt ctaaacaagt cttcaaaaaa ggtacagagt
    22921 atgggttgaa cctaaatgaa gaaaaattaa aaaacgactt aataaaggtg ctggatgttt
    22981 ctatagaaga tatacaaatt gataaatagg agttgggaga atgttgaaaa acttaaatga
    23041 caaagataag agaaaggtat attcactaat aactatagca ggaatatgtg tggtttcatt
    23101 agtattatta tcttgttttc ctagtagcaa aagcgataaa caagttggaa aaacagagac
    23161 taataaaaat gctgaaaagc aagtcactaa agaacaagaa aaagatgatt tagagtcaaa
    23221 attaacagca atactatcta aaattgatgg tgcaggagac gttgatgtaa tggttacatt
    23281 tgaatcaagt gaagaaatac aacctgcttt taattcaaat aatacaacag aaacaacaga
    23341 ggaaaaagat gcacaaggtg gagaaagaac agttacgact tctagtgaaa ataaaacaat
    23401 gataacttca aattcaagtg accctgtagt tataaaaact acagagccaa agataaaggg
    23461 agtgatagtg gtagcaagtg gagcaagtga cccaagtgtt aaagagacac tttatagtgc
    23521 agtacaaaca tcattacaag tagctggtca tcaagtagaa atatattcta aataaataca
    23581 tataaccggg gggataaatg aatgaagttt aattataagg gaagaggatt tgtaataata
    23641 actttaactg caatgttagt ggtagtagga acagtgaatt atcaactaag taagaaatct
    23701 ttattagaaa catcaaaaga gtttaaggca tatgaagaag cacagttgca aaaaaatact
    23761 gatgagagtg atagtagtaa gacagaggat tctagtaatg tagataaaca agatggtaag
    23821 gaaagtgctg atattgatat agttgatagc aaagctagta aagtaaaaga aaaggcaact
    23881 gagacaagta aggaaataaa agctcaatta tcatcagaga aaaatatgaa aaaggcatct
    23941 tatattttag atatgaaaat gaatagagaa aaacaaagaa atgagttagt tcaagattta
    24001 aatgaaatga taaataatcc atctacaaca gaagaatcta gaaaagaagc ttctaatatg
    24061 aaattaaata tagttaaaat tcaagaaaaa gaacttcaaa tagaaaacct tttgagtaca
    24121 aaagggtatg aagaagcgtt agtttatata agcgataaca aagttaatgt agttgtaaat
    24181 gaagctaaat tagaaaagaa agatgcagca aaaatatttg atttagttgc agaacaagcc
    24241 aatgtgaagt atgaaaatat taaacttaca aataataata gtaataagta aagtataaac
    24301 ctcagaatta actgaggtta ttttcttgca aagtagactt gtatttggta ttattatact
    24361 tataaaaata gttccttaaa atcaggaggt aagttttaat atggaagata ataaatttgg
    24421 acaagtaaaa atatctaatg atgtaatagc tacaatagct ggtttagcag cattagaagt
    24481 tgaaggcata gaaactacag caacattaac agataagttg ttaaaaaata atggagtaaa
    24541 aatacaaatc gaagaagagg atgttaattt agatgtaatg gttacgataa agtatggaat
    24601 gtcaatacca gatacagctt ttaaagttca agaaaatgta aaaaatactg ttgagacaat
    24661 gacaggactg aaggtttctc aagtaaatat acacattcaa ggaatcagct ttaagaagga
    24721 taaagttgat aaagaagaag caaaagcaac taagaaaaat taatattagc cctctgaaaa
    24781 gtcagagggt tttttgctgg taattgattt tatatggata attaaactta aaggagaatt
    24841 ttgattgatg aaaaaagata gggcacaaaa gagtacaaca agagaatata taatgaaact
    24901 tatataccaa ataaatatta ataaagaaga ttttgaaact ttagaagata aagtagataa
    24961 ttttttaaaa gataattcag aacatattat aaatagatat aaagaacttg cacttcaata
    25021 ctcaaaaaat acaaatttaa agttagaaga tactgaaatt gaagatgtta tagacaaaaa
    25081 atatataaat acagtatgca aagcattgaa agaaaatcat gacaaaatag atgagttaat
    25141 aaataagcat gctaaaaact ggacagtaga tagaatgcca aaggtagatg tttctatact
    25201 tagattgtct gtgtgtgaaa tactttattt agacactcct aataaagtat caataaatga
    25261 agcagtagaa cttgcaaaaa tatattgtga tgataagtct cctaaattta taaatggtat
    25321 attgggaagt gttgttgatg aaattgggaa ataatataat aattggaatt gatactagct
    25381 gctatactac ttctatagca gctatctctt tagataagaa agttatattc aatgaaaaaa
    25441 taatgttaga agtaagggat aattcaaaag gtttaaggca aagtgaagct gtatttcaac
    25501 atatcaataa tttagggatt cttagtgata ggataaaatc ttttaaagat aagtttaatg
    25561 tagaaggagt ttgctcatca aaaaaaccga gacctgttga aaactcatat atgcctgtat
    25621 tcaatgtagg tcataatttt ggaaaattat tgtctagtat atatggttgt aggttttatg
    25681 agactactca tcaagagaat catattgaag caagtttatt aaatagtaaa ttaaaaaata
    25741 ataataaatt tatttctgtt catatgtctg gtggtactac agagatactt ttaacaagca
    25801 aacaagatag tcatcacaat gtatgtgata ctaatttagg taaaattgcc aaaataagta
    25861 ttaaaaaaga tgataaaagt aagttataca ataattttgg atataatata gatataattg
    25921 gaggaagtaa agatattagc tttggtcaac ttatagatag agtaggaatt aagcttggat
    25981 ataaatttcc ttcaggaaag tatttggatg aaaatgcttt aaattgtaat cttaaaatag
    26041 aaagtggtct gaaaacttct gtaagagatg ggtatatgaa tttatcagga ttagaaaatc
    26101 aggtaaataa gattataaat gataatggag ataatactaa tcaaaaagaa tatatatcta
    26161 aattggtatt agattcagta gttagaaata tgtttaaatc tttagtgtat ttatgtgaaa
    26221 cttataatgt aaatgaagta gtatttgctg gtggagtttc tgcaagtaag tatattctta
    26281 gagaattaag catgaagtta agaaaaaagc atatagaagc ttattttaca gaaccacaat
    26341 attcaactga taatgctgtc gggtgtgcta taataggatt gaataacttt ttaggagaaa
    26401 gagtatgaaa ctaagagctt tagatataag tgaagcaaac tcatatatta aaagaatttt
    26461 aataaacgac ccaatacttt ctaatcttaa agttaaagga gaaatttcta attttaaggt
    26521 tcatagtagt gggaatgtat atttatcact taaagatgaa acatcaaagt taaactgtgt
    26581 aatttttaaa agtaatttta atcgcaattt aaaattagat aatggtgtaa aaattatagc
    26641 taatggatat atatcagtct atgaaagaga tggtgcgtat caattataca ttaatgaaat
    26701 tgaaatagag ggaataggaa atttacacat agagtttaat agattaaagg aaaaacttaa
    26761 taaagaagga ttatttgacc ctaaatacaa gatacctata cctaaaatgc caaactcaat
    26821 tggcgttata acatctccaa caggtgctgt tataagagat ataataaatg taataaaaag
    26881 aagatatccg aaagttaata taaagcttta tccagtaacg gttcaaggag ataaatcagc
    26941 tgaagaaatc tgtgaagcaa ttagattttt caatcatatg aagaatgttg atacacttat
    27001 agttggtaga ggtgggggtt cgatagaaga actttggtct tttaatgatg aaatggtagc
    27061 aagagaggtt tttaattctc aaattccgat aatatcagca gttgggcatg aaacggattt
    27121 tactatttgt gactttgtgt ctgatatgag agccccaacg ccatcagcag cagctgagat
    27181 tgctactcca tcattggatg atataaacta taaacttgga aacataaaaa gtaggatgag
    27241 taagtctctt acaaatcaaa tagagctaga ccaatatagg ttagaaacag tatttaataa
    27301 gataaataac tatttagatt cgtacacaat aaaagataaa gttatacaat tagacaaaat
    27361 atatgataaa ataatttttg gaatagaaaa taatttaaaa ttagaagatg aaaaattagt
    27421 aaaaattgga gcattactcc ataatttaag tccattagct actatggata gaggttatag
    27481 tataactcaa aaaaatggaa aagtaataaa tagtataaaa ggattaaaaa taaaggatag
    27541 tatagatata gtattaaaag atggaaattt agaatgtatg atagataaaa ttgaaaataa
    27601 agagggatag tatgaattta acctacgaag aagcttataa aagattagaa agtattttaa
    27661 gtgaattaga atctaaaaat gctagcttgg atgaatcttt aagcctatat gaagaaggta
    27721 taagccttta taaacattgt aataagcttt tagatgatgc taaattgaaa ataagtaaat
    27781 ttaatcaatt gggaatagaa gaagacttta aaatagagga ggaataaaat tggaatttaa
    27841 acaatgtcta aaagaaaaag caagttttgt agaaaaagta cttaaagaat atatgcctaa
    27901 agaagaaggg tatcaaaaaa ctgttattga agctatgaac tatagcttaa gtgctggagg
    27961 taaaagatta agaccaatac ttacattgga ggcttgtaaa atagttggag gtaatgaaga
    28021 tgaagctatt ccttttgcta ttgcaattga aatgatacat acatattcac taatacatga
    28081 tgatttgcca gctcttgaca atgatgattt aagaagggga agaccaacaa atcataaagt
    28141 atatggagaa gctatgggga ttttagcagg agatgctttg ttaaactatg cttttgaagt
    28201 aatgcttgca ggctcaatca ataaagaaaa tccagaaaag tatctaaaag ctataaatga
    28261 aattgccaaa ggtgctggta tatatggaat gataggtggt caagtagtag atgtagaaag
    28321 cgaaaataaa caaattggaa aagaaaaatt agattatata catatgaata aaactgctgc
    28381 aatgatggta ggctgtatga gagcaggagc tactataggt ggagcaaatt ctgaacaaat
    28441 ggaagaaata actaaatatg caaaaaatat cggattatca ttccagatag tagatgatat
    28501 actagacata gtaggagatg aagctaagtt aggtaaaaag gttggaagtg atatagaaaa
    28561 tcataagtca acatacccat ctcttttagg tttagataaa tctaaagaaa ttgcacataa
    28621 tttgattgat gaagcaaaga aaagtataga aaaattatct gatgatgtag attttttaaa
    28681 gggattggct gaatatataa tagatagaga atactaaaat atacagctaa aatcgaggag
    28741 ggaaaaatgg attttttttc ggagattttt aacaatgggg ctttgggaat aagtctgatt
    28801 gcttgtttct tagcacaatt tataaaaata tttactggta aggaaaaaag aattgagtta
    28861 tcaagaatac ttatttcagg aggtatgcct agttcccata gttcttttgt tacaagttta
    28921 gctacagtgg taggtattga aaaagggttt aattctactg attttgctat aattaccgtg
    28981 cttgccttaa ttataatgta tgatgcagca ggtgttagac gggctgttgg taaacaagct
    29041 actatattaa atcaaatggt ggcagatata cagcatggaa agcatataga gcagaagaaa
    29101 ttaaaagaat taataggtca tacaccacta gaagtttggt ttggagcttt acttggaata
    29161 gtaacagctt taatattgat gtaaattgaa aatttacaga ataaaggtga ggataatatg
    29221 tataaatatt tagataaagt aaattctcca aaggatataa aaaatatgag tatagaagaa
    29281 atggatttac ttgcaaagga tataagaaag ttcttagtaa aatctgtttc aaagacagga
    29341 ggacacttag cttctaatct aggagtggtt gagttaacat tagcacttca taaagtattt
    29401 gatagcccta aagataagat tgtatgggat gtagggcatc aatcttatgt tcataaaata
    29461 gttactggaa gaaaagattg ttttgtatct ttgagacagt ttaatggact aagtggtttt
    29521 ccaaaggaga atgaaagtcc tcatgatatt tttgatacag gacatagcag tacatctatt
    29581 tctatagcta caggtattgc ctgtgctagg gatataaaaa aagaaaacta tagtgttata
    29641 tctgtgatag gagatggttc tataacagga ggtatggcac ttgaagcttt aaatcaatta
    29701 ggatatatag ataccaatat gatagtaatc cttaatgata atgagatgtc catagataaa
    29761 aatgttggag gaatgtctaa atatctgtca agtattataa gaaactcaac agttgagaaa
    29821 atgacagatg aagttgataa aattttaaat gttacacaaa caggtgaaat tttatctaag
    29881 actgcacata gatttaaaga taaactaatg tatagctttt ctcctcagga ctgttcattt
    29941 tttgattcat taggtataag atattatgga cctatagatg gccataatac taaagaacta
    30001 atagatatat tgagaaaagc taaacacaaa aaagggcctg ttcttttaca tgtaattacg
    30061 aaaaaaggaa aagggtacag atttgcagaa gaacaacctg ataaatatca tggagtatca
    30121 aagtttgata taaagacagg tgttacatct gcaaaagtta agtctatgtc aattagtgtt
    30181 ggagaaaaac ttgttgatat ggctaatagt aatgaaaata tagtagcaat aacagctgca
    30241 atgccatctg gtacagggtt aaacctgttt gaaagtgcat atccaaaaag atattatgat
    30301 gttggtatag ctgaacaaca tgcaacagga tttgcagcag gtcttgcaaa aaatggtatg
    30361 aaaccttact ttgctgtgta ctcatcattt ttgcaaaggg catatgacca agttattcat
    30421 gatgtatgca taacaaaaaa gccagttact tttcttatag atagagctgg attagttgga
    30481 aatgatggag aaactcatca tggtatgttt gatttaagtt acttaaattc tattccaaat
    30541 attgtagtaa tggctccaaa agacacaaga gagatggagc ttatgatgga tttgtcatta
    30601 aaattagatt gtccactagc aattagatac ccaagaggaa gtagttatta cctagataag
    30661 ggagagtatg gagagattgt actgggtaaa tatgaggtat tagatgacgg tcaagataca
    30721 gtcatacttt gtattggaag tatggtaaaa catgctttag aggctaaaga aatattatca
    30781 agagaaggaa taaatccgac aatagttaat gctagattct taaagccaat tgatgaaggt
    30841 atgttaaaag cattattaaa aaatcataaa aatgtagtta ctatagagga taatattgta
    30901 actggaggat ttggaagtag aataaataag tttattatag ataatgaata taacgtaaat
    30961 atacttaata tagcaatacc agaagaattt gtaaaacatg gcaatataga tgaattatat
    31021 gattttgtag gattatcgcc aaaaagtatt gctgataaaa taaggaaatt agttattgag
    31081 taaaaatcta atgtagatat tgtaaattaa aatttatata aaatatagaa tttatcaatt
    31141 agaataaata tcaagtgtgt tacttcaagc tactttagtt atcattaaag tagcttttaa
    31201 cagaaaggta attgtatgaa aaaaagaata gatttattat tagtagaaca agggtacttt
    31261 gaaagtagag aaagagccaa aaaagcaata atggctggat tggtttttgt tgataatcaa
    31321 aggtgtgata aggctggtac agaagtaaaa gaagattgta gtatagaagt aaaaggaaat
    31381 ccaataccat atgtcagtag aggtggttta aagcttgaaa aagcaatgaa aaactttgat
    31441 ttgactattg atggaaaggt atgtatggat ataggggcat ccacaggtgg ttttacagat
    31501 tgtatgctca aaaatggagc aataaaagta ttttctatag atgttggtta tggtcaactt
    31561 gcatggaaat tgagacaaga tgatagggtt gtgtgtatgg aaagaaccaa tataagaaat
    31621 gtgacaatag aagatacaaa gcagtttgct gattttgcgt caattgatgt gtcttttata
    31681 tcgttaaagt tagtacttcc aaaggcaaag gagttagtac gtcatgatgg ggaagttgtc
    31741 gctttaatta aacctcaatt tgaagcagga agagaaaaag taggtaaaaa aggagtagtt
    31801 agagaaaaat ctacacatat agaagttata aaaatgattt ctgatttctc agtcgaaaat
    31861 ggatttgaga ttttagggtt agatttttct cctattaaag gtcctgaagg taatatagaa
    31921 tatctaattc atttgagaaa tggaaatgaa ggatatgaat ttgatggtga gacatacaat
    31981 aacaaaatag tagaagttgt tgaagcttct cataatttag acaaataggt taaaaggggg
    32041 cttagatttg atcctcgaat tatatatgaa aaattgtgcc ttagttgaag aactaaggtt
    32101 gaatattgat aaaaacttaa atatacttac aggtgaaact ggttcaggaa aatctataat
    32161 aatagatgca cttggacttt gtttaggtga taaatatgat aggtcttttt taagaaaagg
    32221 aacagataag ggacttgttg aggctgtatt tttttcagac aatagatatc ttaaaaaaat
    32281 attagaagaa aatgatataa gtatggaaga tgataattta ttggtaataa ctaggttgat
    32341 atactcagat ggtaagagta ctgctagagt aaatggaagg actgttaaag tgtctcttct
    32401 taaagaaatt gcatctacac ttatagatat acatggtcaa catcaaaatc aagctttgtt
    32461 taataaagat actcatttaa aatttctaga cctttttgga gaaaatgaat tggaagagtt
    32521 taaaattgct tataaaaatg tgtaccataa gtatagtgaa gtaaaaaaag ctttaaattg
    32581 cttgacagaa aataaagatg agatgcaaat tcaacgagaa atagatttat taagatttca
    32641 aataaatgaa attgaagctg caaatctaaa taagaatgaa tatgaagatt tattaaaaca
    32701 aagagaagtt tatagaaata gtgaaaagat atataataat ctaaattcga gttatagcaa
    32761 gttacataat ggtgagtata atgttattga cctaataggt cttgcatcta aggaacttaa
    32821 tgacatatct aaatatgaca gtgttttaag tgaatatagt gatactgtag aaagaatcat
    32881 gtatgagtta caagacatat ctggtgagat aagaaattac aaagataata tagactttga
    32941 accttatgag ctagagcaga ttgagcttag aatcgacgag attaataact taagaagaaa
    33001 atatggagac agtatagatg atatatttga gtattatgga aaaattaaag atagattaga
    33061 tgaaatatta aacagagatg aaagagttga acagttaaga agcaaactga tgaatataga
    33121 agaagattta aaaataaaag ccagtaaatt gactaaagca agaatagaag tagcaactac
    33181 tttagaaaaa atacttcttg atgaattaaa aagcttaaat atgaaaaatg taatgttcaa
    33241 ggttaatttt gaaaaaggtc catttacttt agatggagta gatgatattg aatttatgat
    33301 atcttttaac ttaggagaag atattaagcc tatatataaa gttgcatcag gtggtgagat
    33361 gtccagattt atgttagcgt ttaaaaccat attggctgat atagatgata ttgatacatt
    33421 agtgtttgac gagatagata ctggaataag tgggatagca gcacagatag ttggtgagaa
    33481 attgagtgat atagcaaaga aaaaacagat aatatgtata actcatttac ctcaaattgc
    33541 tgcaaatgca gacactcatt attgtataga gaaagatact tcaaataata ggacgtttac
    33601 caatgttagt aagttaaatg aatctcaacg aaaaaatgaa attgccagac ttatagcagg
    33661 taacaatata acagaaaaaa ctattgaaca tgcaagtgaa attattgagt tggctaaaaa
    33721 atgttagtga aaaacaagca ttttaatagg tgtaaagaat ttttatagaa atgctttaca
    33781 tctatttttt atgatgaaat tattttaaaa tatctagtgc ctatattgaa gaaaagtaga
    33841 tttaaaaata gaagaaaatt tgtataaatt tgtaactttg aaatttctaa atgcttaagt
    33901 ataatattat ttattaaggt agtagtgagg gggacataaa atgaataaaa aagatactat
    33961 gcacttaatt tttacaattg taatttttat tttattatgg tatattgtgg tagcagtaag
    34021 tgagattaat aaaaatattg aaagttgtag taataaatta gattatttgt cacaagagat
    34081 tatgagtact aaatcggata ttagtaatac tataaatgaa gaaatgtcaa aaagttatat
    34141 aacaaagagt attgacttaa aagtaaaaaa aatcgagaaa aaagagtgtg ttattgatgt
    34201 agatgttcaa ttaagtaaac ttggaaaaaa tggaaaagta ttttttatgt ataaagaaga
    34261 tagtgataaa tggaatgaaa ctgagatgac aaaacatgga gaattatcat atgcatgtga
    34321 aattaagata aatacaggaa gtgaatatga ctataaagta gttacaagtg gagctatatc
    34381 tgaaagttct gatgtgcaaa aattgactaa atctgattat atgcctgaac aacccatatt
    34441 taattctggg ataagagata atagagaata ctttattgaa atagtagaaa atagtaatct
    34501 tttcaatcat gagagtgaaa agtcaaaaaa taaagtttat gataatatta gattagaaaa
    34561 agtagatatt attgtaaatg aaggaaaaaa agacactata tacaaagcca aattagctga
    34621 aggcttagat gatggcaaaa ctaataattc aaatagaaat caagtgaatt atgaagtaag
    34681 ttttcctaaa agggatattg atgacataat ttatattaaa gctaaactta catacaataa
    34741 tggagtagaa tatacaaagg atataactga agatataact atgggattac aagatttaga
    34801 aaaataaata catatttaaa taaatattca aatagatttg ttcaataaaa tagagtataa
    34861 ataaaactgt agtagtttta cttatactct attaaaaaat tataatttta attccatata
    34921 tctagcccct tcaattggat tataagtata aggttctata tcataaaaac caaacttttc
    34981 atataatcct tgtgcacttt tcatgcttgg aagagtatct aatctcatgt gtgtatatcc
    35041 aaatttttta gcttcttcaa tgatttcttc caatagtatt tttcctattt ttaatcctct
    35101 aaattgattt cttacatata atctttttaa ttcacatatt ttaccttcaa gtttttttaa
    35161 agcaacacaa cctgccaaat tttcatcaac aaatgccaat atcaatgagc ctgaaggttt
    35221 tttatatttt cctggaagtg tttttaattc attattaaag tcttgaaaac ataagtctat
    35281 atttagagaa tttgaatatt cagtaaatag aagttttacg ttttcaaggt tttctaaatc
    35341 tttttcttca acaaattgta aagttatatt ttgagccatt ttatccgcca ttatataaac
    35401 atgtttatat aatgacttca cctccttgat atatgtatat ttttcttaat atcaattaga
    35461 ttttaatacc attacttttt caccaccata gtcaagctct ccattaaact caaatccaaa
    35521 atctttataa agcttaatag ccattttatt gtcttcgaat atactaagat atagttcatc
    35581 acagttatat tcatttttaa gatgatttac aagaaaatct aatgatgctt ttccatatcc
    35641 tttaccttga tattttgaat caatcataaa tctatcaagc caaactctat catcttctaa
    35701 acgaccatac atagcaaatc ctattaaaga attttcatca tatattccaa caggtaccca
    35761 agctgttata aatttagatt cagcaataga aagtgcattg ctttctatat aatctaactg
    35821 acttttatct acagacaaac taactaaatc atgccaatta tttatgtctg caattcttac
    35881 atttaatttc aagatattac cgcccttcaa actttttgtt acaatctaat actatcatat
    35941 tatgtattaa aaatcactta ttaagatttt aaataaactt tttaaatgaa taaattctca
    36001 ggaaaaagga attataaata cataataata cagcatagga gtagatggat atatgaacaa
    36061 aataaataaa aatttcaaaa atatttggat aatattagga gctgtatcga tttttaccat
    36121 ggttataatt tttaataaaa attgtaatgt taataaagag actcaaatag aagcttttac
    36181 aattaagaat catgataaaa aatttgtata tcctatggga aatataattg gagtaaaagc
    36241 aaatacagat ggtgtattgg tcttaggata tgaggaagag gatgtagact atattggagg
    36301 catacaaatc ggtgataata tagtaaaaat agataataaa aggataaaaa atagtcaaga
    36361 tgtatcagag attttgaata agattaaaaa aagtaaagtt gaagtgactt ttgagagaaa
    36421 aaataagtat aaaacagaag taatagagac taaaaaagaa aatggcaagt ataaacttgg
    36481 attatgggta agagataaaa tatctggaat tggaactatg actttttacg accctagtat
    36541 ggaaaagttc aaagcaatcg gtcatgccat aaaagattcg gatactaatg aactattaaa
    36601 gataaagcaa ggatatatat ataagccaga acaattaaaa atagtaaaag ctagtaatga
    36661 aaaagtaggt aaaataaaag gagattttaa tgacagcaat ttaatgggta atttctcaaa
    36721 taattcagag ttaggtataa gtggtaatat tacagaaaac cataataaag agtttaatgt
    36781 agcaaataaa gaaaagcaat taatagaagt tggaagaccg caagatgtaa aaataggaga
    36841 tgcagtaatt ctttttgagg ataaaaacaa aaatataaca agctatgata taaaaataga
    36901 aagtatagta tatgataaag gaaattatag agatatggta ataaaagtag tagatgacaa
    36961 gttattggaa tacacaggag gtatcgtaca ggggatgagt ggagctccaa taatacaaaa
    37021 taataaaatt attggtgcaa taactcatgt ttttagagat aatccgaaaa aaggatatgg
    37081 tatttttata gatgaaatga taaaattgta ggtgaggcat taaaaaattt tattatttta
    37141 tcaattatct aggaggaata taattttgga gtgtcgaata tgctttagag tagataatta
    37201 ggaagcaatt gtgtaaaaag tttagttttc tgtaataaga agatgttttt taatgggggg
    37261 atttttagtg gaaaaaatca aaatagtttt agcagatgac aataaggatt tttgtcaggt
    37321 attaaaagag tatttgtcta atgaagatga tatcgatata ttaggcatag ctaaggatgg
    37381 aattgaagca ttagacttag taaaaaagac acaaccggac ttattaatac tagatgtaat
    37441 aatgccacat ctagatggat taggtgtaat tgaaaaattg aatactatgg atatacctaa
    37501 aatgccaaaa ataatagtac tatcagcagt aggtcaagat aagataactc aaagcgcaat
    37561 aaatctagga gcagattact acatagtaaa gccatttgat tttgttgtgt ttataaacag
    37621 aattagagag ctagtttcta atagagttac acaagttgag ccaaaaccta gaccagttca
    37681 agagactcaa atgactagaa gtgattttgt taagaatgta ggaaatatag agacagaaat
    37741 cacaaatata atacatgaaa taggagtacc agctcatata aaaggatatt tatatttaag
    37801 agaagcaata aaaatggtta ttgataatgt tgagctttta ggtgcagtaa caaaagagtt
    37861 atatccaagt atagctaaaa aattcaatac aacaccaagt agagttgaaa gagcaataag
    37921 acatgcaata gaggttgcat ggagtagagg aaaagttgac acaataaatc aattatttgg
    37981 atatacggta cacaatacta aaggaaaacc aactaattca gaatttatag caatgattgc
    38041 tgataaatta agattagaac atagtatggt taaataaaca agacataaaa agtaaggctt
    38101 tttaattaag gcattggcta taaatgcgta ttacaagcag cgaaacggta taaccactag
    38161 ggttataccg tttcgctatt ttaataaata taaaaatttt ctttattatt tgcttactat
    38221 atcaatataa taattttatt atactatgga tatagtatgt gtctttacaa gttgtaaact
    38281 gacagtggtt tattttttaa tataaatatt gactttgatg caggtaaact ttgtattttt
    38341 aagcgtatta tggaatatgt taaataaaaa aatgatgaaa tatagtattg taaatgccaa
    38401 agatgcaaaa caaacttaaa acatttattt tattgttaag taatgctata atataatgtg
    38461 attttaataa tgatagtgga ggtttaaata tgagagtcga ggcccctata aaagtagatc
    38521 gaaaaaccaa aaaacttgct aaaagagttg aaagtgggga aatagcagtt ataaatcata
    38581 tagacataga tgaagttgct gcaaactctt tagtagaagc taaaataaaa cttgtcataa
    38641 atgcggctcc ttctataagt ggtaggtatc ccaataaagg tccagggata ttaactgaaa
    38701 ataatatact aataattgat aatgttggag aagagttatt tgaagaatta aaagaaggag
    38761 aaactataga agttgtagat ggaaagatat atagaaaagg aaaattttta ggtgcaggag
    38821 aagttttaga caagtatgag gtttcacacc aaataaaggc tgcctatgag aatttagctg
    38881 tagaattgga tagatttata gataatacta tagattatgc taaaaaagaa aaaggattta
    38941 tacttggaga agtagaaatt ccaaaagtga aaactaatta tgctaataaa catgtattga
    39001 tagttgtaag aggtcaggac tacaaagaag atttaagtac tatgctttct tatatagaag
    39061 aggtaaagcc agtattggtt ggtgttgatg gaggagcaga cgctttaatt gagtttggat
    39121 atactccaga tgtgattgta ggtgatatgg atagtgtaag tgatgaagct ttaaaaaagg
    39181 ctagtgaaat agtagtacat gcttatacag atggaagagc accaggtctt aagagggttg
    39241 aggaattagg cttagatgca gttgtatttc ctgcgccagg aactagtgaa gatatagcta
    39301 tgcttatagc ctatgagtat aaggctgagt taatagtagc agttggtaca cattcaaata
    39361 tgattgattt tcttgaaaaa ggaagaaaag gtatggcaag tactttttta gttagactaa
    39421 aaataggttc aaagcttata gatgctaaag gcgttaattt gttatacaga agtaaattaa
    39481 aaataaagta catatgggca ttaatagcaa cagcattatt tcctgtgttg gtagttgcat
    39541 cattgtcacc aggagttcaa cagttcatac aactaatgca attaaagttt agagtattat
    39601 tacagatgta aattttagga ggataaatat gcatattaat atgaaatatt atatagtcac
    39661 tataggggct atatttatag ctttgggtat aggcatgtta gtaggattta atttaaataa
    39721 taatcaacaa ttaagtgaac aacaagcaaa tataataaat gatttggatg ataagtttaa
    39781 tatcttaaaa gaaaaaaatg ataaacttga cagtgactta gcaagtgtaa atagagatta
    39841 tgaagaggca gttaatttta taaataaaaa tgtagataag attttagctg gttcgctgaa
    39901 tggtaagagt ataggaataa tatctgctaa tgaaaatgac gattatacaa aaaatataga
    39961 agacataata aataaatcta atggaagcat tgcatttaat ataatattaa aagaaaacat
    40021 aacaaatcca gaaaagttaa aggaaatgtc aactaaacta ggtacagatg taaaaaatgc
    40081 aaatgatgca gttaattata taattgacac tcttaaaaag gaagatgcaa gtgacatttt
    40141 aacatatctt caagaactag atgtaataaa gtttaatttt attggagata catatctaaa
    40201 atatgattcc gttgtaatag ctggtggaaa tgatgcaaaa gacagtacaa aacagtttga
    40261 aaaaatagaa aaatttgtag tttcaaaact taaatcagag aataaatatt tagtagaagt
    40321 tcaaaataca ggagttaaaa cttcttatgt agaactatat tctaaaaaca aagttgcaac
    40381 tatagacaat attgatgaag gtataggaag tatatctcta gcgattcttc tacaacaagg
    40441 taatatagtt ggtaattttg gtagattgga tactgcaact tcattgttgc catctattaa
    40501 atagaaataa ttttaaggag agaaaaaatg gattcttata taagtataat aataccagct
    40561 tataatgaag aaagtagaat aaaatataca ttagaaagca tattggacat taagcaaata
    40621 aatgaaataa tagttgtgga tgatggttca attgacaata ctgctaaggt tgtatctgag
    40681 ctagaaaatg aaaagattaa attttttaag ttagataaaa atagaggtaa aggatatgct
    40741 ttaaattatg gtatcaagtt agcaatgaaa aatgcagata ttataggttt tctggatgga
    40801 gatttaggaa gttctgctaa agaagttgaa aaattaatag tacctatatt aaatggtgag
    40861 gcggatgtta caatagccaa gtttcctcct gcaaagagaa aaggaggttt gggatttgta
    40921 aaaggtttag ctaaaaattc tgtgtttgag atgactggtg ttgagttaga tgcaacttta
    40981 tcaggtcaaa gaatatttaa aagagaagtt ttagaaaagt ttgatgaaat accttttgga
    41041 tatggagtag aagttggaat gaccattgat atattaaagt atggatttaa aattaaagaa
    41101 gtattagtaa atatgactca taatgaaaca ggaagaaatt taaaaggttt tattcataga
    41161 gggaaacagt attatcacat aaaaaaggtt ttaggacaaa aaaagaaaga atggaggtaa
    41221 tatcttagtg aaagattata tactttatac cattttatta ttaactggat tatttggaac
    41281 ctatgctgta attccactat ttaaaaattt attgataaat ggaaatgtac ttagaccaaa
    41341 ctataaaaaa gatatgatac cagtaagtat gggaattgta tttttgccta tgattgtaat
    41401 caatggaata atacttgctt tctttactac agaatttaag aatttagcgt atatatttat
    41461 gtttatgttt ggtatgatat ccatgttttt tgcagggata ctagatgatg tcattggaaa
    41521 tagagatgta agtggtttaa aggggcattt taaaagttta tttaaaggaa gtcttacaac
    41581 aggaggtttt aaagctttat ttggtggatt tgtagggctt atagtttctg ttgctatatc
    41641 aaaagacata attgatataa tagtaaatac actaataata gctctatcaa ctaatttaat
    41701 gaatcttttg gatttaagac caggaagggc aataaaagca tatttattca taatggtaat
    41761 tgtattttta acattgacag gatttgttca agttcttcca ttacttatag taccaaatgt
    41821 acttgcatac tttaattatg atttaaaagc aagggctatg atgggagata ctggttcaaa
    41881 cgtccttgga atatctattg gaatgctcat ttcttttggg tatccattta acattagatt
    41941 aggatggtta atatttgtag ttattatgca tatattcact gagaaatact cattaactaa
    42001 gataatagaa aaaaataaac tattaaattt tatagataaa ttgggaaggt agtattatga
    42061 taagcaaaag agtggggatt gttgaatcaa tagtaagtca aactgaaact ctagatgata
    42121 taagagtaaa tataaatgga gaaatacaaa aggcatataa ttatccaaaa atatctggaa
    42181 ctataaatat aggtgacgaa gtggttttaa atacaacagc agtagaactg agcttaggta
    42241 caggtggata ccattttgta ataactaatt taaataatat agaatctact ttaactgaag
    42301 gtggacatat aatgaaactt agatatacgc ctcttcaaat aaaagtggat tctgttgagg
    42361 aacaagaaag tatttatcat gataaatttg cgaattttaa aggattagaa ggtctttctg
    42421 ttgtagtagg gactcttcat agtatgttaa ctccatttgc agcttcattt aaaagaaata
    42481 atccaaataa aaaattagtg tatataatga ctgatggagc atctcttcca atttatttaa
    42541 gtaaaaatgt ggatacttta aaagaaaaaa aattaataga tagtacaata actataggaa
    42601 atgcgtttgg tggagattat gaatgtataa atatatatac tgcattaatt acagcaaaag
    42661 aaatactaaa agcggatgca gtatttgtca gtatgggacc tggaattgct gggacaggaa
    42721 caaaatatgg atttacaggg attgaacaag gctctattct tgatgctgta aaaaaacttg
    42781 aaggaagagc aattgcaatt ccaagaatta gttttgctga caaacgagaa agacatcaag
    42841 gtatttcaca tcatagtatg actgtattta aagaaattgt aaatgtaaat gtagacatac
    42901 caataactat atatgatgat gaaaaattga actttataaa agagcaaatc aatttaaatg
    42961 gtttagacaa aaagcataat ataatttata tagataataa taaaacgaaa gaggatttag
    43021 actattttaa tctaaaagta aagagtatgg gaagaaatta tgaacaagat gaggaatttt
    43081 tcaaagctgc aagcacagca gcatattact taatggaggt ttaagatggt attagaagaa
    43141 aaaactatta gtagtgatag ggtttataca ggtaaagtaa ttactttaaa ggttgatact
    43201 gttgaaattc cagggcaagg atatcaaaaa agggagttag tagaagttgg tggagcagta
    43261 ggaatagttg caatcacaga tgataataaa gttgtgttag ttaagcaatt tagaaaacct
    43321 atagaaaagc caatctttga aattccagct ggtaaactag aaaaaaatga aagtccaaaa
    43381 gaatgtgctg aaagagaatt aaaagaagaa actgggtata gtgcaaaaaa tattaagcta
    43441 atacataaat ttttcacttc agcaggtttt tctaatgaga taatgtttgt atatttagca
    43501 acaggtctta ctccaggaga gaataattta gatgcagatg agtttttaga tgtttatgaa
    43561 attgagcttg aagaagcata taacatggta ttaaaaaatg acgttgaaga tgcaaaaaca
    43621 tctattggat tattattagt aaaagatatg tttaaaaatt aatatttata aaatgaataa
    43681 ccctttgtcc taaagcatat tattttataa aggacaaggg ggaattaaat tgagaagaac
    43741 atatagaaaa aatgatttta gagaattaaa tagacaaata attattatag gattgttgtt
    43801 tatgatgtct attgttgttg ggtcttacat aaataaaata ttgccaggtt ctagtaacaa
    43861 tatattaaac aatataaatc ctgctgtaga gtattataat ctaaatataa gcataaaaga
    43921 tacagtgata caaaatttaa aatcagatgc aatctttatg ggctcaatag ctctattaag
    43981 tctatttgta gtaacaattc cagcagtgtt ggtagctttt gtattaaaag ggatgtcaat
    44041 tggttataca ataaatagtt gtatattggc attaaaattt aaaagtataa aaatgatttt
    44101 aataatatta tttaaaaatt tgattattat tccatgtgca atcatattag cattaattag
    44161 tttaagttat tttaaagaaa tggtttatga atttaaaaag aaaagtagaa aaaatatgca
    44221 atttttaatt aagaggtata tactaaatat tataattata atagcactat cattggggtt
    44281 gcaattaata ttaaatactg caagtattgg tattattaag ttcctagcta aataaaggag
    44341 tttataatgg atattataga gggatatata gattacataa aagataaaaa aaaattatca
    44401 gagaatacag tggcatcata ctttatggat ataaaaaaat atatggaata tataaatcaa
    44461 aaaagcatta aactagtaga tatagtagaa aatgatatta taagttactt aataaagttg
    44521 gaaaaagata atgtatcaat agctacaata gcgaggatga tatcctctat aaaatctttt
    44581 catgattatt tatttttaaa tcatatatgt acaaataatc ctgcaaagga tataaaaaaa
    44641 cctaaaataa aaaaagaaaa tataaatata cttacagaag aagaaattga aagactatta
    44701 aattttccaa agttaacaac acctaagttg attagggaca aggctatttt tgaagtatta
    44761 tatggtactg gtataaaagt atctgaatta gtagagatga atatagagga tattgaccta
    44821 gatattgatt atatatattg taattcatgt tgtaaaaatc aaagagtgat accactttgt
    44881 gatattacaa aactttactt agaaaagtat ctaaaagaag caagacctaa aatggctgca
    44941 gaaggtgaaa aatcattatt cgtaagttca ttaggtcaaa ggtttacacg acaaggtctt
    45001 tggaaagtca taaaaaaata ttctaatcta gccaatatag ataaaaatat aaatcctaca
    45061 atgcttagac attcatttgc tattcatttg ttaaatgaag gtgctaacat tgctgttgta
    45121 agtaaaattt taggaaatgt taatttatct agtttgcaag tatatttaaa tcatatagat
    45181 aaaaacgtga gaagagaaat aaaggagaaa catcctagaa atgatgttga tgtagaatta
    45241 caaaaagcag aagctaaata gactatatag caaattttat aatttagaag gagcatattt
    45301 atatgagcag agtaatttgg attgtaattg atagtgttgg gataggagca ttacctgatg
    45361 ctgaaaaatt tggtgatagt aaggatgtaa gtacccttgg aaatatattt aaggaatatc
    45421 cagatattca gattcctaat atgagaaatt taggtatagg aaacatagaa ggtatagatt
    45481 tttttgaaag tataaaagaa ccaataggtt gttttggaaa gtgtaaggaa atgtctcagg
    45541 gaaaagatac tactacaggt cattgggaaa tgactggtat aatagttgac aaaccattta
    45601 agacatttga acatggattc tctaaggaga taatagaaga gtttgagaaa aaaactggaa
    45661 gaaaagtagt aggaaataaa ccagcttcag gaactgtaat aatagatgag tatggagagc
    45721 atcaaataaa aactggagat gtaatagtgt atacatcagc tgatagtgta tttcagattg
    45781 ctgctaatga agaagtaatt cccctagaag aactctataa tatgtgcaaa atcgctaggg
    45841 aaataatgat gggggataat gcagtagcta gagtaattgc tagaccattc ataggtaaga
    45901 aaaaaggtga gtttgtgagg acttcaaata gaagagatta ttcactagac ccatttgaac
    45961 caacagttct tgataatata aaagaatcag gacttgatgt tttagcagtt ggaaaaatag
    46021 aagatatttt taatggaaaa ggaattacag atgctatcca cactaaaagc aatatggatg
    46081 gagtggatga aactttaaat tatatgaaac aagataataa aggtctaata tattcaaatc
    46141 tcgttgattt tgattctaaa tatggtcata ggagagaccc agaaggatat aagaaagctc
    46201 ttgaagagtt tgatagtaga cttcctgaaa taatggctaa tatgagagaa gatgatattt
    46261 taataatcaa tgcagaccat ggaaatgacc caacatataa aggtacagac catacaagag
    46321 aatatatacc tgttatgatt tatggcaata agattaaaaa aggttttaat ttaggagtaa
    46381 aagatacttt tgctgatatt ggagcaactg ttgcagatat actaaatgta aagttgccta
    46441 aacatgggtc aagctttaaa gaagatttat tttaaactat aaaatgataa aaacttagct
    46501 taataggatg gagagattgg aatgtttgaa aagatagcac aaagtagtaa atttataaat
    46561 tctaaaagta atataaaacc taagattggt ttgatattag gttctggact aggtgattta
    46621 gcaaatgata tagaagaccc tgtcacaatt aaatatagtg aaattccaaa cttccctgta
    46681 tcaacagtag caggacatgc tggacaactt gtaattggaa aacttgaagg aaaagaagtt
    46741 attgctatgc aaggtagatt tcattactat gaaggatatt ctcagaaaga agctactttt
    46801 ccagtaaggg ttatgaaaga attaggagtc gagattctta tagtaactaa cgctgctgga
    46861 ggcgtaaata aagaatttaa agcaggagat ttgatgctta taagagacca tataaacttt
    46921 agtggaagta atccattagt aggaaaaaat gatgacagat ttggtgctag atttccagat
    46981 atgtcagatg catattcttc taaatatgta gatgtagtta aaaactgcgc taaagaatgc
    47041 aatatagatg tcaaagaagg cgtttatatg ttttttagtg gtccaaacta tgaaacacca
    47101 gcagaagtga gaatggcaca aattttggga gctgatgctg taggtatgtc tactgttcca
    47161 gaagtcatag tagcttctca ctctaatata ggagttattg gtatatcttg tataacaaat
    47221 atggctgctg gcatcttaaa tcaaccacta agtcatgagg aagtaataga aactactcaa
    47281 aaagttaaat cagaatttat gaatttagtt aaaacaacgg ttaaaaattt ataagttaaa
    47341 gcttattata gattttttga ggaagtttat aataaaatag ttaatgttta atttgaaaag
    47401 aattaataaa aagctgtctt gatatagaaa gttatttcta ataaaggaca gtttttttat
    47461 aatactacaa catttgaaag tgttttaaag tttaatattt atttaaatta ttataggtta
    47521 gaatggagat ttcaattatg agaatgtatg atattataaa aaaaaagaga gataacctag
    47581 agcttagtaa aaaagagata aactacttta tagaaaacta ttcaaaggga gatatacctg
    47641 actatcaagc ttctgcacta ttaatgtcga tttatttaaa taagatgaat aaacaggaaa
    47701 ctgtacatct tacagaagca atgatgaact ctggagacat gataaaccta tctgagatag
    47761 atggaataaa ggtagataag catagtactg gtggtgttgg agataagact accatagctc
    47821 ttataccttt ggttgcttca tgtggagcac cagttgcaaa gatgtcaggt agaggtcttg
    47881 gtcatactgg tggtactatt gataaattgg agtctatacc aggtttttcg acagaaatgg
    47941 agataagtaa atttattgat tctgtaaata agtctaaaat agcagtttgt ggtcaaagtg
    48001 caaaggtagc aattgcagat aagaaaatat atgctcttag agatgttaca gcaactgttg
    48061 ataatatatc attgatatca tcaagtgtaa tgtctaaaaa gttagcttca ggagctgatg
    48121 caattgtgct agatgtaaaa actggtgatg gagcatttat gaagacatta gaagagtctt
    48181 ttgagttagc taaatctatg gttgatatag gtactggtat gaatagagat acaataggga
    48241 ttgtaacaga tatggatgaa cctttaggtt ttgcagtggg aaactcttta gaagttatag
    48301 aggcaattga gacattaaaa ggaaatggac ctaaagacct tgttgagcta tgcgaagtat
    48361 taggagcata tatgttggtt ttatcaaagg tagcacatga ttttgaagat ggtgttgaaa
    48421 aaataagaga atcaataaaa tctggtactg ctatacaaaa actcaaagag tttatagaga
    48481 atcaaggtgg taataaagat gtagtagatg attattcttt attcaaaaaa tcaaagtata
    48541 aatatccagt taaatcaact aaatcaggtt atataagcaa aattaaagct gaagatgttg
    48601 gtgtatcggc tatgattctt ggagcaggta gagaaactaa agatgatatt ttagatttat
    48661 cagcagggat aattttagaa aagaaagtag gagattatgt aaatgaaggt gaagttttag
    48721 cttacatgta ttataatgat gaacaaaagc tttcattggc aaagggaaga tttattgatt
    48781 cctactctat tgtagattca aaaatagaaa aaaataaatt aatatatggt attgtaacta
    48841 aagaagagat taaaaagttt tagaaaattt tagaaaagtg attataaaag gcctaacatg
    48901 tcaaaaataa tgttaggttt tttatttttt gataaactat aaattatacc aaaaatatat
    48961 attttaaaac attttttaca atataaataa gtttattcta caagatgatt gaaaattaaa
    49021 gtatctagtt ttataataga aaatggataa atatacataa ggagtagatt atattatgaa
    49081 ctttaaagaa aatagattga aaatagcagt attaataatt gttattttga ttatcttagc
    49141 aggtatattt gtgtttatac aaataggtcc ttatgataaa aataacaaaa aagatgtaat
    49201 tatcgacgtt ccaagtggag catctgttgg aaaaatatct gacatattat atgaaaataa
    49261 attaataaag aatgaactat tatttaaact tttggtaaag gttagcaata aagctccgtc
    49321 tataaaatca ggaacatact tacttaatca atcgtattca aacaatgata taataagtct
    49381 tttagtttca ggtaaaatat atcaggatgg tataaaagtg actattccag agggagctac
    49441 atctaaagaa attatagcaa tgttagtaag taaaaattta ggagataaag ctacttttga
    49501 aaatttaata aaaaaacctc aagaatttta tgataaattt tcatatttga aggaagatgg
    49561 aataacctct ttagaagggt tcttgtatcc agaaacttat tattttaatt caaagaaaca
    49621 atcagaagaa gatattttaa gcgaaatgtt aaaagtattt gacagtaaat acacggacaa
    49681 gtttaaaaag aaacaaaaag aattaaatat gactcttcaa gaagtaatgg aaatggcatc
    49741 aataattgag aaagaagctg ttcttgacaa agacagacct ataatagcaa gtgtgtttta
    49801 taatagactt aaggtaggta tgcctcttca atcagatgct acaatacaat atatatttga
    49861 agagagaaag aaaattgtaa catatgatga tttaaaaatt gattcaccat ataatagtta
    49921 taaaaataag ggattacctc ctacaccaat atcaaatcct ggtataaaat ctattgaagc
    49981 agcattatat ccagagaaaa cagactattt atattttgta gctaagattg atggtggaaa
    50041 taattatagt acaaactatc aagaccattt aaaatacgtt aaagaatata aagaagcaag
    50101 ggataaacaa tcaaaagata caaaagcaac aaataaagaa aatacaaaaa aataaaccat
    50161 gattataaaa agtctaacag ggtaagaata atgttgggct tttttattga aaattgttat
    50221 tcttagattt ataataaaga aagtgtaaat atatataaca atatgaattt aaaaaataat
    50281 aaattaatat aaaacttgga tatttattat tttagggagt gtttttatga gtaatatagt
    50341 taatgatttg gttgaagatt atataagagt gacattaaaa gaaaaagaag gatttttaaa
    50401 agatttagaa atttatgcag aggaaaatag tgttcctata atacataaag aagtttcaga
    50461 tttattaaaa gtcttgttaa aggttcaaaa accaaagaga attttagaag taggatgtgc
    50521 aattggttat tcttctattt tttttgcaac tattatagga aaagatgcag atataataac
    50581 tgttgagaga aatgaaaaaa tgatagaaaa agcaaaagaa aacataaaat tagctggatt
    50641 tgacaataac ataactatac tagaaggaga tgcagaagaa aaacttagtc aagtccaagg
    50701 tgaatttgac cttatattta tagatgcagc caaaggtcaa tataagctat tttttgattt
    50761 agttatagat aaattaaaag atgggggact tttagtatct gacaatattt tgtataaagg
    50821 tatggttgct catgatgatt ttgttgtaag aagaaaaaag actatagtta aaagaatgag
    50881 aaagtattta gattatatat gtaattgtga ctatttaagc acatcattgg taccaatagg
    50941 tgatggtgta gcactaagtt ataaagaaag taaaagaggt gatgtagatg gaattaaata
    51001 aagtagaact tttagctcca gcaggtgatt tggagagatt gaaaatagct ataacatatg
    51061 gagcagatgc cgtttatata ggtggagaaa tttttggaat gaggtctgca gcgaagaatt
    51121 ttagtaagga agatatggca gagggagtag cttttgcaca tgaaagaggc aagaaagtat
    51181 tcgtaacagt aaatataata cctcataatg aagattttct acaattagaa gactatttgt
    51241 tggaactaga agagatagga atagatgcag ttataatagc tgacccagga gtgcttagtg
    51301 tcattaaaaa agtaattccg aatatggaaa tacatttaag tacacaagca aatacaacca
    51361 attatctaag tgcaaacttc tggtacgagc atggaataaa aagagttgta gtggcaagag
    51421 aattatcttt tgatgaaata tatgagataa gagcaaaaac acctttagat atggatatag
    51481 aagcgtttat gcatggtgct atgtgtattt cttattctgg cagatgttta ataagcaact
    51541 atatgactgg aagagatgca aacaaaggtt cttgtgctca atcttgtaga tggcaatatc
    51601 atttggtgga agaaaaaaga cctggtgagt attttccaat atacgaagat gaaaggggta
    51661 cattcttctt taactcaaaa gatttatgta tgatagagta tataccagag ttaatcaaat
    51721 ctggtataac aagtttaaaa atagaaggta gaatgaagac tgcatactat gtagctactg
    51781 ttgtaagagc atacagaatg gcaatagatg aattttacag agacccagaa aactggaagt
    51841 ttaatccaat gtggatggaa gaactaaaaa aaggtagtca tagacatttt acatcaggtt
    51901 tttacttgaa taaaccaaca acagaagacc aaaattatca atcagcatca tatgttagaa
    51961 actatgattt tataggtata gttagagaaa cagaagatga agatggtctt attgttgttg
    52021 agcaaagaaa taaaatgtgt gttggtgatg aaatagaagt tatgggacca tataaagaaa
    52081 ctatgtttac taaaattgaa gaaatgtata atgaagaagg agaagctata gaatcagctc
    52141 ctcatcctag acaaatagtg aagttaaagc tatcagtaaa agttggtaaa gattatatgc
    52201 ttagaaaagt tatagaagaa aaggttgaag aataataaaa aattgataaa aatagtatta
    52261 gccccttaga aattgtcaat aatgaaatta tctaaggggt gatttcatgt caaaaaagaa
    52321 gactccattt ttgaaaaaag tgggaaagag aagctggtgt atatttacta taattttaat
    52381 aatttatagt gttttaattt ataggctagt tgatatacaa gtattaaaag gtgataaata
    52441 caaacaaagt gttgaatcac aaagcgttga gaaagtcgag ttaaatagtg gaagaggaat
    52501 aatctatgat agaaacaaca agaagcttac agatacaagt aaatctcagg tattaattgt
    52561 tgaaaaagaa aaattaaata ataactataa aattttggag cttattaaga aagctactaa
    52621 gatgaatgat ttagatatat ataaagctgt acaagagcaa ttgacacgcc ctataataca
    52681 gattcagact aaaaatatcg atatcgataa gagtatgaaa aaagaacttg agaaaaatgg
    52741 aataatggta gaagaaaaaa ctatgaggta cgcaaaagat ggtttattat cacatacaat
    52801 aggatatata aaagaagatg ataaatcagg tcaatcagga attgaaaaga gtatggatag
    52861 tgtacttaga aattcaaatg aaaagtatat aagtgctttt aaagctggag atgcaggaaa
    52921 tgaaaaatct ctaaacatac taaagggaag tgtaaaaact gttgataata aggataaaga
    52981 tagacatcta aaaactacta ttgattataa cattcaaaaa aaattagaac aaatactaaa
    53041 taaagaagaa aatcctacag cagcaataat ctcggaagca tctacaggtg aaattcttgc
    53101 aatgtgctct agacctaatt ttgaccaaaa tgacatatca aaaagtttaa aaggaaaaaa
    53161 tggagaattt gaaaatagag ttataaaagc gacatatcca ccagggtctg tatttaagat
    53221 ggtagtttta ttttctgcac ttgaaaatgg tgttatagat gagaactata cttacaactg
    53281 tacaggtaag actaaagttg ggaatactaa tgaaatactg cgatgtaata aaagagatgg
    53341 tcatggtttc caaaacttaa gacaagcttt ttctaattca tgcaatcctg catttttaga
    53401 tatagctatg aaacttggaa aagaaaagat actcaaatct gctgaaaaat tacatttatt
    53461 tgaaaaggtt gatattggtc ttgatgaaga aaaaattaga gaagctccaa aaaacatatc
    53521 aataagaaat ttagctatag gtcaagaaaa tatagaattt acaccacttc aaataaatca
    53581 aatgactcaa ataatagcta ataatggaac attcaaacca ctatacttat ataaaagtct
    53641 tgtggacaat aatatgaata caataaaaac ttataagtct tcaaaaaaag aagaacttat
    53701 ttctccttat gtgtgtacac aagttaaaga atatatgaag tctgtatcta ggactggtac
    53761 agctaaagac ctaaaagata ttgaaggtgg ttgtggagtt aaaactggta cagctcaaag
    53821 tagtttaaat aaaaaagcta tagaccacgg atggataaca ggattttatc ctgaagaaag
    53881 acctaagtat gtaataactg tgttagtaga aggaactcaa aaaggaaata aatctgcaac
    53941 acctatattt aaggaaatat gtgaaagtat aaaataaatt atgtactata aaatttaagg
    54001 atatatcgta actttgattt tagatagaaa attttgtcta atgatgtacg atatatcctt
    54061 ttaaaatata gtattaactt tcttgaacaa gctctttttc tagtttttta agagcttttt
    54121 tttcgattct agatacatat gaccttgata tatcgagttt ttgtgcaatt tctctttgtg
    54181 tcttatagcc ataaggagtt aacccatatc ttaactggac aatttctctt tctctaggag
    54241 taagaatttt gtttagttgt tcgtaaagtt ttccaacttg aacttttagt tcaacttcat
    54301 caagaacata gttagcctct gtaccaagta tatctaaaag acttatttca ttaccttctt
    54361 tatctgttcc gatagggtct tgtagagata cttgagtttt attttttttg ttagatctaa
    54421 tattcattaa aatctcattt tcaatacatc tagaagcata tgttgctaat cgagttcctt
    54481 tatctatgtt ataagtttct atagctttaa ttaatcctat tgtacctatg gatattaaat
    54541 catcctgatc ttccgttgaa ttattatatt tctttgctat ataagctact aatctcatgt
    54601 ttctttcaat taatgtatct tttgcacttt tatcgttttc tattttaaat ttagttaaat
    54661 actcaatctc ttcttcaggg gttaagggtt tttcaaaaga tttaagagct gccaataggt
    54721 cacccccttt tcaacggtca ctttaaatta tatgaaggag acttatccaa gttgactgtt
    54781 atctaataaa ataatatgca tatttttaga atttaaaaca tatatttgca aacttttgaa
    54841 tggaatatga cataaagagt gggaatttaa atttaagttt ttgttgtata tatatgaaat
    54901 aaggcgaaga aattattgat ttataaagta taaattggaa ttgaggtgtc aataaatata
    54961 aaaggaaata gtatttatat atgttttggt gttgaagtaa aagattaatg caaagcatta
    55021 gagtctataa aaatatgaat ataggaggtg taattttaca atacttaaaa aagtaaatgt
    55081 aattaattgt ttgtataaag ttgattacaa gcaaaagtat cagtcatagt taatactgtt
    55141 aagggtgaag gtggtgtgtt aatttatata tgtatgggaa gaaattttga ttacaatgaa
    55201 aaaacttaca aaagatattt atgaaaaaac ttatagttaa atgtttatat ataaaatatt
    55261 atttaggggt tgtttttatt attgaaaata agaaagagtg gttttatatc aattgaatgt
    55321 ataataagta ttgctatatt atatgtggct gtttatttag tttctacatc attgtataat
    55381 tgttatagtt ttatcagtag aaatatatct gacagagaaa tgttaagtac agcaaaaaaa
    55441 tatatagaag atgagaagta tagaatacaa aatagtaagt atgagttaat tgaagataag
    55501 atagaaaaaa attacataaa tggatatgaa attaacagta aaatagagca aattttagat
    55561 tattatcaat gctatgaaat aaatatagag ataaaaaatg aatttaaaaa actgaggttt
    55621 aatagctatg ttactagaaa ataaaggctc tatattaata ataacattga taatattttc
    55681 tattataagt actgtgtgca ttatgtgtat aggtttaatt tatacacata ataatatgtt
    55741 tgagttagga tacaaggaca ttcaactaaa agaaatttct ttaagtggaa tagaaatatc
    55801 agtaagtaat gtactttctt gtgttgagga agctattgat gtatctaata atgaagacga
    55861 atttagagaa tatttcttag gaaataatat gattgaatgt ataaaaaaaa taaaagatac
    55921 atcttatagt agtttagaag aactttcttt aaaagtagaa aataatacta tttatgacag
    55981 aggagatttt tttgaatttg atatagaaag taatgttaga gaaaaagaat ttttaaaagc
    56041 aataaaagta agagtgaaaa tcaaaaatcc atggatagga attgacattg aagattatat
    56101 ggataaatct aataacaata aaattgatga caaaacattc aataattata aagcatctga
    56161 aaataatgaa attctaaatc aatttaaaaa tgaaagtgaa aatataagga atcaagaggt
    56221 tggtggattt acagaagaag atgaagaaga tgaagaatat gaagaagaac ttaaacaaaa
    56281 cataattgaa aatataaaga agaattttga tgaaaaaaac ctagtacagg tatataagta
    56341 taggggtgta taactttgaa aaaagaagga atgtgactgt aatagaatta atagtatcta
    56401 tcagtataat actcttgata gttacattat cttttccaaa agataatctt gaaaatcata
    56461 aaataaattt atttgcacgt caactttgtt cagatataag gtatgttagg cgaattaata
    56521 tgtttagcaa ctacaataca tatatatatt atataaatca aggtaaacac gaaggttatg
    56581 tattgagaaa agatggtaaa aacattaaat caataatgct acctaaaaat gcaaaaatag
    56641 ctaggagtat tgaaataata aagtttaaat ctgatgggtc gcctcaaaag gcgtctacaa
    56701 tagagatata taataataga ttaagaaaaa ctataacaat aacacctgta agtggaagag
    56761 tacttctgaa agagggaaaa tatgaaacat aaatatggtt acttgctttt ggaaagtgtc
    56821 gtatcattgt cttcaatggt gataattata ctagttttat attctatatt tttgtctaca
    56881 ataaacttaa agttaaaagt agaagataag atagaactac aacaacaaag tcttgaaatc
    56941 ataaaatcta tggaaggaat tattagcaat tctatgggaa taatgaatgt atctaattat
    57001 gaagaaacat ttaaaaagac aacatctata aaatgtagat atttagatga aaataataat
    57061 gaagaaggca taagtaataa agaaattata ttgaatgaaa gacgaaataa gttatttgta
    57121 aattctttaa atggcgaaag tagtcaagca ggtggttatg aaattggaga ttatgtagat
    57181 gagatgtatg tatcaattac taataatggt caatatgtta atattaaatt gaaattatca
    57241 aaacgtagtc aaaaatatga gactgatttc aaaataaaag tatggaattt tagtgaaagt
    57301 atatagaaat acataaattt aagagaaaga agtgacatca ataagtcata taaaatataa
    57361 ttattgagaa ctatataact aaataatatc aatatgtaaa gtgtatagat taagaatata
    57421 gaggataata ataaaaatat agaataagta atttgccaaa tacttgttta tattgtataa
    57481 cttatgatat tatgatatta gtaaaaattt gtaatcctga cttgacagaa ttcaagtaag
    57541 agatataatt ttaaaggcat agtaatgtaa aatttactaa aaaataagaa atttatttta
    57601 atatggagga acaaaaaatg gtatcagcag gtgattttag aaaaggtgtt acatttgaaa
    57661 aagatggaca accatgttta gtagttgatt ttcaacacgt taagccaggt aaaggagctg
    57721 ctttcgttag aactaaatac aaaaacttaa aaacaggagc tataagagaa gaaagtttca
    57781 atccaagtga aaaattccct aaagctgtta tagatacaag acaaatgcaa tatctataca
    57841 atgatggtga gttatattat tttatggatc aagaaaattt tgaacaaata ccattaaact
    57901 atgagcaagt tgaagatgca attaagttct taaaagaaaa tgaagttgct acaataagat
    57961 tttaccaagg acaaccattc caagtggaag cgccaaactt tgcagagcta gaagttacag
    58021 atacagagcc aggtataaaa ggtgatacag caagtaatgt aactaaagca gctacagttg
    58081 aaactggagc agttgttcaa gttccattat ttataaatac tggagataaa gtaaagatag
    58141 atactagaac aggtgaatat ttatcaagag tataaaaatt tatgtatata aaagcttata
    58201 tgtcttaata atataagata tatgagcttt tagtttatat ataaaaaaga aagcgagggg
    58261 tgttacatga aacatttata tgaagaagtt gcatacttaa aaggcctagc agaaggacta
    58321 gaaataagtg ctgaaagtaa agaagggaaa atgatacata aaatagttga tgcattagaa
    58381 gtatttgcag atgctattgt aacattggat gaagaacaag aagaacttca agattttgtt
    58441 gaatctatag atgaagattt agcagattta gaagaagata ttgctgacat ggaagaagat
    58501 ctttatgaag aagatgacga tgaggatgat gaggacttca gctatataga aatggaatgc
    58561 ccaaattgtg gagagttagt agaaatagac gaagatttgt tatatgatga tgaagtagat
    58621 gttgtttgtc ctgattgtaa ggctgtgata ttatcatcag aagatgattg tgatgatgat
    58681 tgtacatgtg gtggttgttc aagttgtgat gatagagagt aaaaattcat aagaattaaa
    58741 actgcctatg atgcctatga gatattttgc ttcataggta gttttatttt tatgaggaag
    58801 ggtattatgt attttggttt tgcaatactt ggacaaaaat agtacactag aatttataga
    58861 ataggatttt ggagggatgt aaatatgaaa ataagtaaaa aattactaga taatatacaa
    58921 agatttgaaa atgttataaa ttataaattt aaaaataaag aatatatact agaagctctt
    58981 actcatagtt catattctaa tgaaaataaa aaatataatt ttaacgaaag attggaattt
    59041 ttaggggatt cggttttagg catagtgata agtgattatt tatttaatga agaagctaat
    59101 ctgcccgaag gggaactaac taaacttaga gcaaatatag tttgtgaaga ttcattaagt
    59161 gaagtagcta atgatataaa tttaggtatt cacatgttat taggtagagg agaagaagca
    59221 acaggaggaa gacataggac ttcaatatta gcagacgcat ttgaagctgt aatagctgct
    59281 atatatcttg atggaggttt tgaaagtgca agacagttta tacttcatca tatggaaaat
    59341 ataatttatg attcaagaaa aggaaatata tttagagact acaaaacgca tcttcaagag
    59401 gtgttacaag ggaatggaga aaataatatc tggtacaggc ttatagaaga aaaaggacca
    59461 gaccataata aaagatttgt aatggaagtt ggaattaatg acgatgtact tggtatagga
    59521 gaaggcaaga gtaaaaaaga agctgaacag ttagctgcta aaatagcttt aaagaaaaaa
    59581 ttatgggaaa aataaatata atatgtaaaa atataaaaag aaatatttat attagaagga
    59641 tgttgtagat gaaaaagaga attataccta tatttgttcc tcacaaagga tgtcctcatg
    59701 attgcatatt ttgtaatcaa aaaaaaataa caggtgtgag tactgatgtt actagtgaag
    59761 atgcaagaaa tataattgag gaatgtttag agactataga taaagatgca gatgtagaaa
    59821 tagcattttt tggtggtagc tttactgcta tagatattga tatacaaaaa agtttattat
    59881 cagttgctaa agaatatgta gaaaaaggtc ttgtaaaaga tattagaatg tctacaagac
    59941 cagattgtat tgatgaagat atattaagta tgttaaaaga atataagaca agtataattg
    60001 aactaggagt acaatcctta gatgaaaaag tattattaga tagtataaga ggtcatcaat
    60061 ctgaaattgt atacaagagt tcaaagatga taaaaaatag tggaatcaaa cttggattac
    60121 agatgatgat tggtcttcca gctgacacag aagaaaagtg tatatttaca gcaaaaaaat
    60181 ttgtagagct aaaaccagat tgtgttagag tctatccaac tttggtagta aaagatacag
    60241 gtcttgaaaa gttaatggaa caaaataaat ataatccatt tactcttgaa gagagtgtaa
    60301 atatagtaaa aaaagttctt gtactgtttt atgttaataa tatcaatgta ataagagttg
    60361 ggcttcaagc tactgatgat atacaaattg gaaaagctgt tttagcagga ccatatcatc
    60421 ctgcatttag agagttggta gaagcagata tgataaaaga ttatttagaa tttgttattt
    60481 tgcaaaataa aaatataaag caaatgttgg tcaaatctaa taaaaagaat atttctaaaa
    60541 tcataggaaa taaaaaaaca aatgttaaat atatggaaga aaaatttggt gtgcttttaa
    60601 aaacacaaga atcagattta gatataaatc aattagaaat tgtgttagat gggaaaagtt
    60661 taattagtgc taatatgaga gacattcata gaaaattgta tgacatttat gacctttagt
    60721 tttaataatg aagggagaga aaagatttgt atttaaaaag actagagtta aagggattta
    60781 aatcctttcc agtaaaaaca gatattattt ttaaagaagg aataacagcc atagtaggac
    60841 caaatgggag tggaaaaagt aatatatctg atgctgtaag atgggtatta ggagaacaga
    60901 gtattaagag tcttagaggt gataaactcg aagatgtaat atttgcaggt acagatacaa
    60961 aaaaacctat gaattattgt gaagtggctc ttactattga taattctgaa aatcaattgg
    61021 aattagattt tacagaagta actataagaa gaagggctta tagaaatggt gaaagtgaat
    61081 tttttctaaa taataaaagt tgtagattaa aagatataaa agaagtcttt ttagatactg
    61141 gtattggaaa agatggttat tcaatcatag aacaaggaaa agtagatgaa atattaagta
    61201 ataatccttt aagcagaaga aaagtttttg atgaagcttg tggcatatca aaatatagat
    61261 ataaaaaaca ggaagcagaa aggaatttaa gtaatacaaa ggaaaattta gaaagaatag
    61321 atgatgtata tatagaaatt gaaaatcaat taaagcctct ttttaatcaa cagacaaagg
    61381 caaaaaagta cttggaaata agtgaaaaat taaagacact tgaagtaaat agctttataa
    61441 gagaaattga aggaatagaa aaagaattaa gtgaggttaa tgaacatcgt aaggttattg
    61501 aaaaggagct caatgaaaaa gaagaacaaa aaaatgttgt agagaaaaag caagaagata
    61561 ttaataaaga ggtagaggta ttacaagatg taattgaaaa atctgtagat tatataaatt
    61621 caattaaagg agttatatca aaaaaagagt cccaaataaa tttaattaag gaaagaataa
    61681 gaaattttac gaatgaaatt agtagaaaaa atttagaaat aaaggatata aaagagaagc
    61741 taaatgaaaa taagcaatac ataaaagaat tagaaagcaa taaattatca ggtagtgaag
    61801 aactatctac cttgcaggaa aatataaaag tattggaagg aagtaaagat aaacaaaaaa
    61861 ttaagttaga atccctaaat aatgagatag aattattgaa agaaagtata attgatatat
    61921 taaataagaa acaagagttt agtaataagc tgtctacttt aaacgcaaat aaagaaaata
    61981 tgaatatcag agatgaaaat ataaattcag agataacaga gttaaacaaa aatattgaaa
    62041 taaaatcatc tgaattagat actataaata aagaatttaa tatgcaaaat gaaaatttaa
    62101 agaatgtaaa taatagacat aaagaattat caattaattt gcaggattca attagtgaac
    62161 acaataaact agaagatgaa atacagaaaa gtaaatataa tttaaatgga tataattcaa
    62221 agttaaatgt ctatatagat atggaaaatc attatgaagg atttaataga ggagtaaaag
    62281 aagtactaaa aaataaaaat ttaaaaggag ttcatggagc attaggacag attataaatg
    62341 tacctgaaaa gtatgagaaa tctatagaag cagctcttgg tgcttatatg caaaatataa
    62401 taactgataa tgagtttagt gctaagtttg caataaacta tctaaaacag aataatttag
    62461 gtagagtaac atttcttcca ttaaatataa ttaaatcaaa taaaataagt ctaggaaatt
    62521 taaaagcaaa tactaagttc ataggaatag caagtgattt aattacattt gatgaaaagt
    62581 atagaaatat aattgaaaat atacttggaa gaacaatact tataaataat atagatgagg
    62641 gcataaaatt tgcaaaagaa actggtcata ggtttaaaat agtaacttta gatggtgaaa
    62701 tcttaaatcc aggaggttct ttgactgggg gaagtctaaa aactaatggg aatatactat
    62761 cgagaaaaag atacattaat gaatatacag aaaagataag taatatcaag aatgaaattt
    62821 cacatctaga attaaaaaga gaatctttag acaaagatgt gaaaaatatc aaaaatgaaa
    62881 ttgattcaca tgaaagtaaa ataaaagatt tagaaaaaag tataataata aaaagtacaa
    62941 gtataaagaa tgttgagtct gaaattgaat cgttaaaagg tagtattact aaattagaaa
    63001 atgagaagaa tgatttaaat tctaacctaa attatacact tgaaaaaagt gatgatgtta
    63061 gaaaagatat ggaagagtta gatgatttat acaataaaaa taaagaaaaa attgatgcat
    63121 tgaatgagga aataaaaaga tacaatgatt tatatgataa agaaaaatct gaatttgatg
    63181 aattgaactt aagtttagtg aagaaaacag aagtttacaa tagtatagtt agagatataa
    63241 aaagaatatc tggtgaaaat tgtgagctag aagaaaaaaa taaacaatta gaagagtcat
    63301 taaattatga agaacatgaa ataattaaat tacaagattc tatactcact gaagaaaaag
    63361 aaaaagaaaa tttaacaaaa caactagggg atagtaatag aaatcttgaa actagaaaaa
    63421 tagcaaaaga tgatttaaaa aatagttttg atgaaattaa taaagaatta aaaactatag
    63481 atagacagca tatagaactt aaagaaagtt tatttaaagt tggtggaaga ttagaaagat
    63541 taaaaactag tcaagacaca tatataaata agttgtttga gcaatatgat atgaccttag
    63601 tacaagcatt ggaaattaaa gatgaagatt tggatataga tagaaaattc ttagaaagtc
    63661 taaagagaga aataagaagt cttggaaata taaatataga ctctataaag gaatatgagg
    63721 aaataaaaga aagatacgat ttttatagcg aacaaaagca agatttagaa gagtctatgg
    63781 aagagataga aaaacttata catactttag aagaaaatat gaaatctgaa tttgaaatta
    63841 agtttgaaga aataagtaaa aattttaaat atgtatataa gagattattt ggtggaggtt
    63901 gtggagaatt aactatttta gataaagaaa atttattaga aagtgatata ctaataacag
    63961 ctcaaccccc aggtaaaaaa atgaaaaatt taaacctatt atctggtgga gagaaagctt
    64021 taacggcaat aagtatatta tttgctatat tgattacaaa gccaacacca ttttgtatat
    64081 tagatgagat tgaagcgcca cttgatgacg caaatatatt tagatttgga gaatttttga
    64141 aggatttatc taaagagact caatttatat cagtaactca taggagagga acaatggaag
    64201 ctgctgatta tatctacgga gttacaatgc aagaaaaagc catttcaaaa gttattagtt
    64261 taaaattaaa agaagctcag gaaataacag atattatata acttagattg acataggagg
    64321 aataacagtg ttaaaaaaat tatttggttt tggaaaagac aaggaaaagg aaatagaaaa
    64381 aaaagatgct gaagaagaaa tagaagttga agacagtgtt gataatttag aaaacttgga
    64441 agaaactatt ttttcaggac ttgaagaaga agtgatagat aaagtggaag atattgaaga
    64501 aaaaaatgag gaaagtgtca ataatgaagg cactgaagaa atcgaaaact ttgaaaaaat
    64561 agaagttgag atggatagta atggaaaaga agtagaaaat atttcaaata atgattctca
    64621 agaagagata atcgaagaat tagaaaatta tgatgaaatt gagtctaaag agatagaaga
    64681 taaagaagat aagaaagtta acttgtttga aagactaaaa caaggattga caaaagcaaa
    64741 gcaaggaata actgatagaa tagatgaagt tttaaaatca tatacaaaaa tagatgaaga
    64801 attacttgaa gatttagagg aaattttaat aacagcagat gttggtgtaa atacaactat
    64861 ggatataatt gaaagactaa gagataaaat aaagcaaaaa ggtattactg aaccaataaa
    64921 ggtaagagaa gaacttaaat ctatagttga agatattctt acaaatgaaa actctacact
    64981 agatatagaa ccagcaccat gtataatatt aatggtgggt gtaaatgggg tcggtaagac
    65041 tactacaata ggtaaactag caaatagata caaaaaagat ggtaaaaaag ttttattagc
    65101 agcagcagat acatttagag cagcagcgac agagcaatta gaaatttggg caaatagaac
    65161 caatgtagac ataataaaac atcaagaggg agcagaccct ggagctgtag tatttgatgc
    65221 tataaaagct gcaaaagcta gaaaaacaga tgtattaata tgtgatacag caggaagatt
    65281 acacaataag gctaatctta tgaatgaact tggaaaagta tttaaaatag tagatagaga
    65341 atttccagaa gcaaaacgtg aagtacttct tgtggtggat gctacaacag gtcaaaatgc
    65401 agttgttcaa gcaaagacat ttaaagaagt tgcagatata acaggtatag tacttacaaa
    65461 acttgatgga actgcaaaag gtggagtagt ccttgcagta aaatcagaag tagatgtacc
    65521 tgtaaaactt attggagttg gagaaagtgt agaagaccta caagatttca atgcaaaatc
    65581 attttcagat gcattatttg gtaactaatg ttcttttaaa aataatggtt gacaagagtg
    65641 ttgttatgat ataaaataca atagtgtaaa gtaaataacc tttacagtat actgctagga
    65701 agtgtaagat atgaatatag aaaaaatggt cgaaattgga ttgttatttg aacagtataa
    65761 agaattatta actgacaagc agaaagaaat agttgctcta tactatgaag aagattattc
    65821 tttaggtgaa attagtgaaa atctgaatgt atcaagacaa ggtgtttatg ataccttaaa
    65881 gcgttcagaa aaaatactaa gagattatga agaaaagtta cacttagtat caaagattca
    65941 agagcaggaa aaaaatatta agattataaa agataaaatt attgacatta aagaagattt
    66001 attgcacaat agagattgtg ctaatttaat ccctaagcta gaaaatatag aagatgtatg
    66061 tagggagatg ataaaatgat atttgaagga ttgtctgata aactacaagg ggcatttagt
    66121 aagttaaaat ctaaaggaaa acttacagaa gtagatgtaa aaaatgctat gagagaagtt
    66181 aaacttgctc ttcttgaggc agatgttaac tttaaagtag ttaaagattt cataaaaaaa
    66241 gttcaagaga gatgtgttgg gcaagaagtt atgcaaagct taactccagc tcagcatgta
    66301 atcaaaattg taaatgaaga gcttacaagt ttaatgggag atgtacaaag taaagtaatg
    66361 atttcctcaa agcctcctac aattttaatg atggttggat tgcaaggtgc aggtaagact
    66421 actacagcag gaaaacttgg aggatatttt aaaaagcaag gtaaaaaacc tctattagta
    66481 gcttgtgata tttatagacc agcagctata aagcaacttc aagttgttgg agaaaaattg
    66541 gatatacctg tattcaatat gggagataaa gaaaatccaa taaatattgc gaaagcaggt
    66601 ttaagccatg cgattaaaaa tgctaatgat ttagttatta tagatacagc tggtagactt
    66661 catatagatg aagtacttat ggatgagtta aagtctatta aatcagaagt taagccacat
    66721 gagatacttt tggttgtaga ttctatgaca ggtcaagatg ctgtaaatgt tgcagaaagc
    66781 ttcaatgagg cacttggtgt tgatggagta gtattaacta aattggatgg agatactaga
    66841 ggtggtgctg cgctttcaat aagagcagtt acacaaaagc ctataaaatt tatggggatg
    66901 ggagaaaagc tagatgatat agaacctttc cacccagata gaatggcatc aagaatatta
    66961 ggtatgggag atgtcttaag tttaatagaa aaagcacaag aaaatataga cttagagaaa
    67021 gctaaagaat tagagcaaaa aattaaaaaa caagagcttg attttgaaga tttcttacaa
    67081 caaatggaac aaatacaaaa tatgggacct cttgacaaga tacttggtat gatacctggt
    67141 atgggaaatg taaaagacca acttggagat gtagatttga atggcaaaga aatgaagaga
    67201 acaaaagcca taattcagtc aatgactaca tatgagagaa gaaacccagg agtattaaat
    67261 gcttcaagaa agaaaagaat agctaaaggc agtggaacta gtgtacaaga tgtaaataga
    67321 cttataaaac aacttaatga aatgaaaaaa atgatgaaga tgtttgctgg ttcacaaaag
    67381 agtatgaaga aaaggggcgg cttaggcggt ttaccttttt ttaaataaaa tataaaaaag
    67441 taaaaattat tcggaggtga ctgtaatggc agttaaaata agattaaaaa gaatgggagc
    67501 taacaaaaaa cctttctaca gaatagtagt agctgattca agagctccaa gagatggaaa
    67561 atttatagaa gaaataggat actataatcc aatttctgaa cctaaacaag taagaataaa
    67621 tgatgaaaaa gctataaaat ggttagctac tggtgctcag ccaacagaag tagttaaaaa
    67681 attacttgta aaaaacggag taatagaaaa attcgaagct tctaaacaag caaaataatt
    67741 ttttcttggc aattagttta agtaagggct cattgctaag gttactttag gagatgaaat
    67801 atatgaaaga gctagttgta gatatagcta aggctctagt tgataatcct gactcagttg
    67861 ttgttgagga atttgaagat aatgatggta tcgtcttaaa gcttacagtt gctcaagagg
    67921 atatgggtaa ggttattggg aagcagggaa gaatagctaa agctataaga actgtcgtaa
    67981 gatctgttgc aaatagagaa aatataaaag tttctcttga gatagtataa aataggagga
    68041 ttaggtataa tgcctagtcc tttttatata aatgcgtagt ttaagatttt atgatgatat
    68101 gaccacataa gattaaattt tttaagaagg tgataacatg aaagaaaaat taactcattt
    68161 taaggtaggt caaatagtaa atacacaagg tttaaaaggt gaagtaagag tttatccgct
    68221 tactgatgat atagagaggt ttgatgaatt aaaagatttc tatctgggaa aagatttaaa
    68281 taataaattg gctgttgaaa aagtaagata caaaggttct atggttataa tgaagataaa
    68341 aggcatagac agcatagaga aagcagaaaa actaaaaaat aaatttatgt atgtagctag
    68401 agaagatagc agagagcttg aagaaggcga attttttata gcagatatga ttggaatgga
    68461 agttttaact gtagatggaa aatatgtagg gatactagaa gatgttcttc aatactcagc
    68521 aaatgatgtc tatgtagtaa aaggtgagga agataaagaa tttatgatac cagctataaa
    68581 aaaatttgta cctacaatag atattgatga aagaaaaatg attatagacc ctattaaggg
    68641 aatgatagat taggtggtaa tatgagattt catataatga cactttttcc agaaatattt
    68701 aattcgtaca tggatgaaag tataatgaag agagcagttg aaaaaggtat aattgaggtt
    68761 catatatata atataaggga tttttctaat aataaacata aaaaagtaga tgattatcca
    68821 tttggaggag gagcggggat ggtaatgact cctcaaccta tatatgacac atataagcat
    68881 ataataacaa ctcataatat aaataaccca agtgtaattt atcttactcc aaaaggtaag
    68941 gtatacaatc aatctatggc aaagcagatg tctttaaaag aagatataat actactatgt
    69001 ggtcattatg aaggaataga tgagagaatc attgatttaa tagttactga tgaaatttca
    69061 attggagatt atgtattaac tgggggagag ctcccagcac ttataatgat agattctata
    69121 tcgagattaa taccaggtgt attaaaccaa gaagaatcat ttgaagaaga atcatttaaa
    69181 gacaacttat tggaatatcc tcattacact agacctagag attttgaagg attaaaagta
    69241 ccagaagtgc ttttatcagg gaatcataag aagatagatg agtggagaag agaagaatct
    69301 ataaggataa ccaaagaaag acgatttgat ttatataaaa aatctaatga aaaataaaaa
    69361 tataatgaaa aattaatata tatgtataca ataaagtacc tataaatgga attaaaaaca
    69421 taaaagtgct tgtaataact attgaagtat agtataatat aaaatgttga aaatacgggc
    69481 gttcctctgt ttacactggt cgggtagtta agaacgtcta tgatatctag gaggaagaaa
    69541 gatgaatgaa atgctaagag caattgagca agaacaatta aaaaatgaag ttcctaattt
    69601 tggacctggg gacacagtta aagtacacgt tagaattata gaaggaaaaa gagaaagaat
    69661 acaagtattc gaaggtgtag tattaaagag acaaggtgga ggagcaagag aaactttcac
    69721 agttagaaaa atgtctttca atgttggtgt agaaagaact ttcccagttc actctccaaa
    69781 aatagaaaaa atagaagtta ctagaaaagg taaagtaaga agagctaaac taaactactt
    69841 aagaggtaga gtaggtaaag ctgctaagat aaaagaagct agaaataaat aattaaaaac
    69901 tttacttata aatggggggc tgatttatca gtcctctttc tattttaatg aaaattacag
    69961 caaataatta tactagaaat aaatagaaat atttgttgaa aatatacatt atttatctat
    70021 gaatttatag cttttaaaat gaaaaataaa attggtaaat attgcattat attttgtaac
    70081 aatattaaaa ataagagaac agtattatgt aaagtatgac gttaaaaaga tgacaatatt
    70141 atagtttgtt aagatagatt ttttaagtgg aattaggata taataatata atattaataa
    70201 ggagtgatac atgtgtcaaa tgaatacgaa gagtatctta tgaatgacaa tctacatata
    70261 aactggtatc caggtcatat gaaaaaaact aaggagttag taaaaaataa cttaaaattg
    70321 attgatgtag taattgaact tttggatgct agaatcccgt ttagtagtaa aaatccagac
    70381 atagacaggc ttgtaggaga taagccaaga gttgtcgtac ttaataagag tgatatggca
    70441 gatagggata aattgaatca atggattgaa tactataaga aaattaatat caaagctata
    70501 ccagtagata caataaaagg cgttggtata aataagataa tcgaagaatg taaaaatgta
    70561 actagagaaa aaatgagttc tttaaaagac aaagggagaa aagaaagagc tatacgaata
    70621 atgatagttg gagttccaaa cgttggtaaa tcgtctctta taaacaaatt aacaggtaga
    70681 aaaagcactc aaactggaga taagccagga gtaacgaaag ggaaacaatg ggttagatta
    70741 aagggaaatc ttgaactttt agatacacct ggaatacttt ggcctaagtt tgaagaccaa
    70801 gaagttgcat taaatttagc atttagtaga gctataaagg atgagatttt agatactgaa
    70861 actttggctt taagacttat agagaaactt atgaaaatag aacctgagaa gttgaaagcc
    70921 agatataagt tagattgtct aggagaaact ccaattgaaa ctatggatat gattggccat
    70981 aaaagagggt ttataacagg taaaaaagaa ctagattata cacgcatagc cacaactgta
    71041 ttaaatgaat ttagagatgg aaaaatagga aatataacat tggaagttcc agaaaacgtt
    71101 aaaagataga ttttaaaaca aaatcaaaaa tttaatgtta aaaatttaaa aaagcctttt
    71161 agcaaaggct tttttatttt tttgaaatat agacaaatat ctccatataa aagtataaat
    71221 taggttagaa aacctttaaa aatattaatt taagacaaat atacctaata ttttgaaaat
    71281 taaaatgagt gtgattattt aaaattttag catattgact tgcttgtttc gtataatata
    71341 taataagcac aaataaatac aatatattaa aaatttaatt tatttaattt tattaattta
    71401 ataataagtt cttattgttt aattcaactt acttatgcaa gcgtaaacaa tcgagacatt
    71461 ttattaattt aataaggtta aaaaggggga ttttataatg ggtaagacca aggatgttat
    71521 agtatttgga tttgcgttat ttgcaatgtt ctttggagca ggtaacttaa tatttccacc
    71581 ttacttggga ataattacag gtccagaatg gttaattgcc ttcttaggat ttacgtttgc
    71641 tgatgctgga ttggcacttc tagcagtaat ggctacagct aaattcgatg gaaacgtagt
    71701 tgaaatgttt aaaagatgtg gtatgaagtt aggaattctt ataggttgtg cagatatttt
    71761 atgtatagga ccattcttag ctataccaag aacaggtgca acaacttatg aaatgggtat
    71821 aatgcctttg tttggaactt caattccagt gttattattc tgtatacttt tttttgcgat
    71881 ttcatatgta ctaacaataa gaccttctaa ggtcgtagat atagttggac agtttttaac
    71941 tccagctctt ctaatagctt tagcatttat aattataaaa ggtattatat cacctcttgg
    72001 agatatagta gataaaccta tgatacctaa tgtatttgca gaaggtatag gtcaaggata
    72061 tcagacaatg gatgcttttg cagcaatagc acttgcatca gtgctaatag tatctttaaa
    72121 tgataaagga tattctacta ttagtgacaa gttgaaaatg attggtaagg cgggtgttct
    72181 tgcttgtgga ggattagctc tagtttatgg aggattatgt ttccttgggg caactgtatc
    72241 aacaatgtat ggaacagatg cagtacaatc acaagtaata gtaaatataa ctgaaggatt
    72301 gcttggaaat gttgggaaag cgattttagc agtagtagta tcattagcat gtttaactac
    72361 atctataggt cttacatctg ccacaggtca atatttctct agacttacaa aaggtaaatt
    72421 gagttatgaa aaaatagtat tagctgtatc tgtattcagt gcagtagtag catcttttgg
    72481 tgttggaact ataataaaga tagcatctcc tatactaagt atagtttatc caccatcaat
    72541 tgttttaata attttagctt tcttcaatga aaaaattaag aatgataatg tatacaaagg
    72601 agcagtatat atgtcattgt ttgtaagtat actaacagtt atatctagtt atggagtagc
    72661 tgttccagta gtaaattctt tacctcttaa ttcattagga tttaactggg tggtgccagt
    72721 tataatagct ggaataattg gtaattttat accatcaaag agtcaatcta atacacttgg
    72781 aacaaactaa gttttgttta attaaaatat aatcaattgt aaataaatga gttaactaaa
    72841 agtaaaagga
//
LOCUS       DAYATN010000005        72154 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.005, whole genome shotgun sequence.
ACCESSION   DAYATN010000005 DAYATN010000000
VERSION     DAYATN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 72154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.005"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            208..1425
                     /gene="ilvA"
                     /locus_tag="ACGI8A_000316"
     CDS             208..1425
                     /gene="ilvA"
                     /locus_tag="ACGI8A_000316"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HFH0178328.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1520..1900
                     /locus_tag="ACGI8A_000317"
     CDS             1520..1900
                     /locus_tag="ACGI8A_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFH0178329.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2184..2666
                     /locus_tag="ACGI8A_000318"
     CDS             2184..2666
                     /locus_tag="ACGI8A_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFH0178330.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2687..3502
                     /locus_tag="ACGI8A_000319"
     CDS             2687..3502
                     /locus_tag="ACGI8A_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFH0178331.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3553..5103
                     /locus_tag="ACGI8A_000320"
     CDS             3553..5103
                     /locus_tag="ACGI8A_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0178332.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5200..6552
                     /locus_tag="ACGI8A_000321"
     CDS             5200..6552
                     /locus_tag="ACGI8A_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFH0178333.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6683..7441
                     /locus_tag="ACGI8A_000322"
     CDS             6683..7441
                     /locus_tag="ACGI8A_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0178334.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7488..8207
                     /locus_tag="ACGI8A_000323"
     CDS             7488..8207
                     /locus_tag="ACGI8A_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HFH0178335.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8331..9731)
                     /locus_tag="ACGI8A_000324"
     CDS             complement(8331..9731)
                     /locus_tag="ACGI8A_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFH0178336.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9735..10625)
                     /locus_tag="ACGI8A_000325"
     CDS             complement(9735..10625)
                     /locus_tag="ACGI8A_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0178337.1"
                     /translation="MTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKATFYKHF
                     ETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRTTINTYI
                     RENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDALIHQFI
                     YIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYFSLHNKS
                     LFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAYLEKEDT
                     LRPKVLLLNSIKNQLKKETL"
     gene            10904..11551
                     /locus_tag="ACGI8A_000326"
     CDS             10904..11551
                     /locus_tag="ACGI8A_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFH0178338.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11876..14515
                     /locus_tag="ACGI8A_000327"
     CDS             11876..14515
                     /locus_tag="ACGI8A_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFH0178339.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14573..15676)
                     /locus_tag="ACGI8A_000328"
     CDS             complement(14573..15676)
                     /locus_tag="ACGI8A_000328"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFH0178340.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVREAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16036..16857
                     /locus_tag="ACGI8A_000329"
     CDS             16036..16857
                     /locus_tag="ACGI8A_000329"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFH0178341.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIDGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16936..17147
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17234..18550
                     /gene="argH"
                     /locus_tag="ACGI8A_000330"
     CDS             17234..18550
                     /gene="argH"
                     /locus_tag="ACGI8A_000330"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFH0178342.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18866..21094
                     /locus_tag="ACGI8A_000331"
     CDS             18866..21094
                     /locus_tag="ACGI8A_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFH0178343.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(21333..21632)
                     /locus_tag="ACGI8A_000332"
     CDS             complement(21333..21632)
                     /locus_tag="ACGI8A_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFH0178344.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            21838..24879
                     /locus_tag="ACGI8A_000333"
     CDS             21838..24879
                     /locus_tag="ACGI8A_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HFH0178345.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKEAWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(25040..26314)
                     /locus_tag="ACGI8A_000334"
     CDS             complement(25040..26314)
                     /locus_tag="ACGI8A_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HFH0178346.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(26535..29096)
                     /locus_tag="ACGI8A_000335"
     CDS             complement(26535..29096)
                     /locus_tag="ACGI8A_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0178347.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVKNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            complement(29097..29774)
                     /locus_tag="ACGI8A_000336"
     CDS             complement(29097..29774)
                     /locus_tag="ACGI8A_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178348.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQVSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            complement(29854..31107)
                     /locus_tag="ACGI8A_000337"
     CDS             complement(29854..31107)
                     /locus_tag="ACGI8A_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0178349.1"
                     /translation="MKIVFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMV
                     VRNNQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPI
                     ISKLVLETIKINIILVCIIFILIFVLIVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKN
                     KNQEGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLND
                     LLYNDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHR
                     AMLSMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITY
                     KENPLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNG
                     DIYVNSEKGKGTEFHIIFHKMYDSD"
     gene            complement(31085..31810)
                     /locus_tag="ACGI8A_000338"
     CDS             complement(31085..31810)
                     /locus_tag="ACGI8A_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0178350.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            complement(32074..32520)
                     /locus_tag="ACGI8A_000339"
     CDS             complement(32074..32520)
                     /locus_tag="ACGI8A_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178351.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEELDLHEDAKKDNIYKEDEYKKYEEE"
     gene            32712..33101
                     /locus_tag="ACGI8A_000340"
     CDS             32712..33101
                     /locus_tag="ACGI8A_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0178352.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            33094..34185
                     /locus_tag="ACGI8A_000341"
     CDS             33094..34185
                     /locus_tag="ACGI8A_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HFH0178353.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFIPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLLTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            34305..34781
                     /locus_tag="ACGI8A_000342"
     CDS             34305..34781
                     /locus_tag="ACGI8A_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFH0178354.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            35167..35634
                     /locus_tag="ACGI8A_000343"
     CDS             35167..35634
                     /locus_tag="ACGI8A_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFH0178355.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(35694..36266)
                     /locus_tag="ACGI8A_000344"
     CDS             complement(35694..36266)
                     /locus_tag="ACGI8A_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0178356.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(36329..37426)
                     /locus_tag="ACGI8A_000345"
     CDS             complement(36329..37426)
                     /locus_tag="ACGI8A_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFH0178357.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            complement(37440..38129)
                     /locus_tag="ACGI8A_000346"
     CDS             complement(37440..38129)
                     /locus_tag="ACGI8A_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFH0178358.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            complement(38294..39460)
                     /gene="rpoD"
                     /locus_tag="ACGI8A_000347"
     CDS             complement(38294..39460)
                     /gene="rpoD"
                     /locus_tag="ACGI8A_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFH0178359.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            complement(39478..41268)
                     /gene="dnaG"
                     /locus_tag="ACGI8A_000348"
     CDS             complement(39478..41268)
                     /gene="dnaG"
                     /locus_tag="ACGI8A_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFH0178360.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            complement(41523..42308)
                     /gene="aroF"
                     /locus_tag="ACGI8A_000349"
     CDS             complement(41523..42308)
                     /gene="aroF"
                     /locus_tag="ACGI8A_000349"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFH0178361.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            complement(42289..42795)
                     /gene="folK"
                     /locus_tag="ACGI8A_000350"
     CDS             complement(42289..42795)
                     /gene="folK"
                     /locus_tag="ACGI8A_000350"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFH0178362.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            complement(42788..43153)
                     /gene="folB"
                     /locus_tag="ACGI8A_000351"
     CDS             complement(42788..43153)
                     /gene="folB"
                     /locus_tag="ACGI8A_000351"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFH0178363.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            complement(43164..43955)
                     /gene="folP"
                     /locus_tag="ACGI8A_000352"
     CDS             complement(43164..43955)
                     /gene="folP"
                     /locus_tag="ACGI8A_000352"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFH0178364.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            complement(44019..44591)
                     /gene="folE"
                     /locus_tag="ACGI8A_000353"
     CDS             complement(44019..44591)
                     /gene="folE"
                     /locus_tag="ACGI8A_000353"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFH0178365.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            complement(44617..45318)
                     /locus_tag="ACGI8A_000354"
     CDS             complement(44617..45318)
                     /locus_tag="ACGI8A_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178366.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            complement(45352..46095)
                     /locus_tag="ACGI8A_000355"
     CDS             complement(45352..46095)
                     /locus_tag="ACGI8A_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HFH0178367.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDIKYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FVKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            complement(46085..47434)
                     /gene="pabB"
                     /locus_tag="ACGI8A_000356"
     CDS             complement(46085..47434)
                     /gene="pabB"
                     /locus_tag="ACGI8A_000356"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HFH0178368.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            complement(47436..48020)
                     /locus_tag="ACGI8A_000357"
     CDS             complement(47436..48020)
                     /locus_tag="ACGI8A_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFH0178369.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            complement(48044..48763)
                     /locus_tag="ACGI8A_000358"
     CDS             complement(48044..48763)
                     /locus_tag="ACGI8A_000358"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0178370.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            complement(48791..49855)
                     /locus_tag="ACGI8A_000359"
     CDS             complement(48791..49855)
                     /locus_tag="ACGI8A_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFH0178371.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            complement(49999..50808)
                     /locus_tag="ACGI8A_000360"
     CDS             complement(49999..50808)
                     /locus_tag="ACGI8A_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFH0178372.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            complement(51050..51916)
                     /locus_tag="ACGI8A_000361"
     CDS             complement(51050..51916)
                     /locus_tag="ACGI8A_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0178373.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            complement(52436..53125)
                     /locus_tag="ACGI8A_000362"
     CDS             complement(52436..53125)
                     /locus_tag="ACGI8A_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFH0178374.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(53118..53489)
                     /locus_tag="ACGI8A_000363"
     CDS             complement(53118..53489)
                     /locus_tag="ACGI8A_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFH0178375.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(53671..55068)
                     /locus_tag="ACGI8A_000364"
     CDS             complement(53671..55068)
                     /locus_tag="ACGI8A_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFH0178376.1"
                     /translation="MEVSIILVKDIKQDIESMPRKILDISLIEDNKSDLTFFNIEEED
                     GDETLKALKQSYVTTNYKEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKN
                     YITSAINMVNKALEINPKDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKY
                     VNLISSEDFKTFLGRYNHSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDK
                     LGNVKKREGYLDKLKELDKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCV
                     LIGMGIYLIQSKKRIENLNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEP
                     EREITVADEEDLYYQALNLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKL
                     LGNKDEAIKYYKKYINTYTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPD
                     SMYNNSKIKEILSEK"
     gene            complement(55199..55792)
                     /locus_tag="ACGI8A_000365"
     CDS             complement(55199..55792)
                     /locus_tag="ACGI8A_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0178377.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(55857..57326)
                     /locus_tag="ACGI8A_000366"
     CDS             complement(55857..57326)
                     /locus_tag="ACGI8A_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFH0178378.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(57441..58832)
                     /locus_tag="ACGI8A_000367"
     CDS             complement(57441..58832)
                     /locus_tag="ACGI8A_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFH0178379.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(58998..59288)
                     /locus_tag="ACGI8A_000368"
     CDS             complement(58998..59288)
                     /locus_tag="ACGI8A_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178380.1"
                     /translation="MSGLKKILRILVISMMICFAMTFALVLINSVMGVHIGLDYYFLM
                     GSVLTVCLTIGIVLCNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            59512..61641
                     /locus_tag="ACGI8A_000369"
     CDS             59512..61641
                     /locus_tag="ACGI8A_000369"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFH0178381.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(61868..62467)
                     /locus_tag="ACGI8A_000370"
     CDS             complement(61868..62467)
                     /locus_tag="ACGI8A_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viroplasmin family protein"
                     /protein_id="HFH0178382.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(62485..65604)
                     /locus_tag="ACGI8A_000371"
     CDS             complement(62485..65604)
                     /locus_tag="ACGI8A_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFH0178383.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANKIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINFIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            65825..66649
                     /gene="pdaA"
                     /locus_tag="ACGI8A_000372"
     CDS             65825..66649
                     /gene="pdaA"
                     /locus_tag="ACGI8A_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HFH0178384.1"
                     /translation="MKKDSLKKYIMIGAFALTLFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(66746..67531)
                     /locus_tag="ACGI8A_000373"
     CDS             complement(66746..67531)
                     /locus_tag="ACGI8A_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0178385.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEGGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(67631..67765)
                     /locus_tag="ACGI8A_000374"
     CDS             complement(67631..67765)
                     /locus_tag="ACGI8A_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178386.1"
                     /translation="MNISRKAMKIIELAQKIANKRGISVEEAWSEAVTEYKNKYEHIA
                     "
     gene            67996..68361
                     /locus_tag="ACGI8A_000375"
     CDS             67996..68361
                     /locus_tag="ACGI8A_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0178387.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            68439..68993
                     /locus_tag="ACGI8A_000376"
     CDS             68439..68993
                     /locus_tag="ACGI8A_000376"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFH0178388.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYYK"
     gene            69213..69887
                     /locus_tag="ACGI8A_000377"
     CDS             69213..69887
                     /locus_tag="ACGI8A_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFH0178389.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      70242..70338
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            70392..70568
                     /locus_tag="ACGI8A_000378"
     CDS             70392..70568
                     /locus_tag="ACGI8A_000378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178390.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(70739..70978)
                     /locus_tag="ACGI8A_000379"
     CDS             complement(70739..70978)
                     /locus_tag="ACGI8A_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178391.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(71093..71932)
                     /locus_tag="ACGI8A_000380"
     CDS             complement(71093..71932)
                     /locus_tag="ACGI8A_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0178392.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITKYLYFNGL
                     DGK"
BASE COUNT        24838 a        10901 c         9067 g        27348 t
ORIGIN      
        1 tgtaaaaaat atttttaaat tatcatttaa tgtaatttaa atgaattgaa gatatatgtt
       61 tattgtatgc aattgttgta tttttaggtt ttttgtgatt aattaaaaaa ttataaaaat
      121 cgtttacatg ttgattttta aaaaaaaaag atatagaatt atatagaaag taataaacaa
      181 aaattggatt tacgggagtg gaatgaaatg atgaatgtta ccttaacaga tgtgaaagag
      241 gctagagaga ccattaagaa catagtaaaa agaactgact tacttgaaag tgttaaactt
      301 agcgaaaaaa caggagcaaa tgtgttctat aaatgtgaga atcttcaaaa aacaggttct
      361 tttaaattaa gaggagcttg taacaaaata gcgagtttaa cagatgaaga aaaggcaaat
      421 ggagttatag cttcaagtgc agggaatcat gctcaaggag ttgcattggg agcaaaaatg
      481 actggtataa aatctacaat agtaatgcca gcaactgcgc ctttagcaaa agttagtgca
      541 actaaaggtt atggagcaga agtagtttta aatggtgcgg tttatgatga tgcatatgca
      601 aaagctgttg aaatacaaaa agaaacagga gcaactttct tacatccatt caatgataag
      661 tatgttatag caggacaagg aactatatct cttgaaattt tcgaacaatt agacaataaa
      721 gttgatacta tactatgccc agttggtgga ggaggaatca tctctggtgt tgctgttgca
      781 gcaaaagcat taaatcctaa tgtaaaaata gtcggtgttc aaactgcaaa tataccttca
      841 atgaaagagt caataaaaaa tggaaaagta acaacagcat ttaatgatac tacaatagct
      901 gatggtatag ctgttaaaac tccaggagat ttaacttttg aaataataaa tgaattggtt
      961 gatgaaatag tagtggttga agagacagaa attgcagaat caatattatt tatgatggaa
     1021 agtcaaaaaa tagtttcaga aggtgctggt gctgtatgta cagctgctat attaagtggt
     1081 aagtatgtac cagcaaaaga tgaaaatgta gtttgtataa tatctggtgg taatatagac
     1141 ataaatacgt tatatagaat aataggtgtt gctttagcta aagaaggaag aagatattca
     1201 tttagtacaa ttatggaaga taagccagga aactttgctg agttaactag aataataagt
     1261 gagaatggtg gaaacatttt aagtgctaat caaggtaaat tatcagcagg ggaagctcta
     1321 ggaaaacaaa gtgcggaatt tatattagag acaatagatt atgaccatat agctagaata
     1381 aagaaagcta tagaagaaaa aggttttaaa attatagaat tataataaaa agtgtatata
     1441 atatacaaga aaaatagtat aatagtgtat gaagatacat tttatgtgta ttagattaac
     1501 gaattaggag gattttaaaa tgaaacatga agtaatacat actaatgatg caccagcagc
     1561 tttaggacca tattctcaag ctataaaagc tggaaacttg ttatttgtat ctggacaagt
     1621 tcctcttgta ccagagacta tggaagtagt tgaaggagat gtacaagctc aaactgctca
     1681 atctttaaaa aatcttaaag ctatattagc tgaaagtgga gctgattttt caaatgtagt
     1741 taagactaca gtatttataa aagatatgaa tgaatttggg gctataaacg aagtttatgc
     1801 tgaatatttt ggagaaaata aaccagcaag agcttgtgta gaagttgcta gattaccaaa
     1861 agacgttaaa gttgaaatag aagtaatagc tgtattataa aaatacaata tttttaattt
     1921 gaaaaggctg aaaaactata ttctagtgat tcagcctttt tataaaaaaa taaatcacaa
     1981 taaagaacat gataaatatt gtcgaattaa aaaataaaat taaaataaat attgcaatat
     2041 ttttgagaag gttttataat gagtatataa tttgaaacgt gtaactatta aattaggcat
     2101 gagttcttgg atatttttat cctgaggatt catgcctttt ttgtattgaa attcaaaaat
     2161 ctatttaata aggaggaagt actgtgttaa attttttcaa gaaaaataaa agctataagt
     2221 tacatgcagt agtgagtggt aacagcatta atattgagaa agtaaacgat tctgtgtttt
     2281 caaaaaagtt aatgggagat ggagttgcaa ttattcctaa tagtaatgtt gttgtagccc
     2341 cttgtaatgg taaagtaact gtgcttacag aatcaaagca tgcctttggt atggtctcag
     2401 atgagggtgt tgaaatatta gtccacatag gaattgatac agttagttta cagggtgagg
     2461 ggtttaaaaa tgaagtgtct caaggagata ctgttaaaaa aggtagtcca ataataagtt
     2521 ttgaaagaga aaaaataaat tctcagggaa ttgattgtac aacaataata attgtattaa
     2581 atcatagcga attttcagaa ataaattgca tggtagaaaa tgaagttgtt gcagggcaag
     2641 atactgttat tgaaattatg aaataatagg aagggattaa ttcaatatga gagtagtaaa
     2701 aatctttaat aataatgcat tatctacagt aactaatgat aggaaagaag ctattttact
     2761 aggtcttggt ataggtttta ataaacgtcc aggagataaa gttaatgaag ataaaataga
     2821 aaagatttat tatgttcaag accacatgca aacaaaattt ttagaattat taaaaaatgt
     2881 tagtccagaa gtgatggatg cttcacaaca aattataagt cttataccag gaaatgaggg
     2941 aaaatttaat aataaaggga tattatcttt agtggaacat attagttttg caatagatag
     3001 aatgaaaaat aatgtttttt tacctaattt aatgcttttg gatataaaga tgatgtattc
     3061 aaaagagttt gaattaggag taaaagcttt agaaattata tatagagtat gtcatatcca
     3121 tttgcctgaa gatgaagcag gttacatagc tttacatttt gtcaatttac aatcaaatga
     3181 taatttggca tatgatactt taaaatttgt aaaaggtagt attgatttga ttaaagaatg
     3241 ttatggatta gaattagacg aatcgagttt aagtacatta cgttttagaa ctcatcttaa
     3301 atttcttgct caaagaattt ttaagaatga aatttgtcag gatgataaaa tgattgaaat
     3361 gtatgattat ttaattaaca atcatcctaa aaataaagaa tatttagaaa agttaaatgt
     3421 atatattgaa aaggagttta ggtataaatt agataaacct gaaaaaattt atctactact
     3481 acacctaaca aaaatcttat aagaatgaga tgaattagta atagaaatat ataattttag
     3541 ggggggataa gattgaaaga aaaactactt ggaggactgg aaacatttgc aaaggcaatg
     3601 gttcagccat taatgtattt atcgagtgca ggaatattaa tggtggtagg tgtactactt
     3661 acaaatgata tgttattagg agtacttcca ttttttaaat gggagccaat tcaaatattt
     3721 ggaagcctaa tttataactg tataatggct attattaata atcttgctgt tatatttgct
     3781 ataggtattc ctgctgcact agcaaaaaaa gagaagcaca aagcagcgtt aataggattt
     3841 atgagttata taatatacct tacagcttcc aacactatgt taacatcact aggtcaacta
     3901 gcaaaagaaa gcaagatgct tggactgata gggactggac aagctactat tttaggtatt
     3961 caagttgtag atacaagtgt atttggtgga attattttag ggtgtatagc tggatatgta
     4021 tttaatcgta caagcaacaa aacatttaaa ggagctttac agatatactc gggagcaaat
     4081 tttagtttta tatgcatgtt ctttgtatcc attttaattg gagttgtgag taactttata
     4141 tggccattag ctcaacaagg aatagcttct cttgcaaatg tcatcaaaga tacaggtaca
     4201 tttggtttat tcttatatgg ttttttagag agaatattag taccaactgg acttcatcat
     4261 ttaatctata caccatttca gttttctgat ttaggaggag tgcttaaagt tggtgcagat
     4321 acagtttcag gagcctatgc tatagttatg acagaaatga atttaccagt tgagcgtttc
     4381 tcagatagta tatactacat ggcaactgga tttacaaaga tgtttggtta tataggtata
     4441 ggagctgcct ttatttatac tgcatataag caaaataaga agaaagttaa ggcaacgatt
     4501 attccactaa taattacggc agtattagcc tctgtaacag aaccattaga ctttatgttt
     4561 gtattctgtg caccattttt attctttgta catgcagtta tagctggtct ttttatagct
     4621 ttattaaaaa tatttgatgt tactgcatta tgtggaggga acttattagt atcaattata
     4681 atgaatacta cagtaggcgt agaaaaaaca cactggccag taatgatgat attaggactt
     4741 gcacaaatta ttgtatattt tgtagtattt agttttttaa ttaagaaatt taattataaa
     4801 actccaggtc gtgaagatga gagtacagct acaggcgaag aacaatcaaa acaacttaat
     4861 ttagatgcag ggatagaaaa tataattgat gggttaggcg gaaaagaaaa tattaataca
     4921 gtagaaaatt gcatcactag attacgtgtt aatgtaaaag atgaaagtaa gattaatgaa
     4981 gatataatta atttaacgcc aaatagcggg attgtaagga aaggaaaaga tatacaaata
     5041 atttttggac ttcatgtgca tgaggtaaga cgtgcagtag aagatttttt agaagcttat
     5101 taaagatata tatttatata gcaagggtaa ataacagctt taagtgctgt ttactattta
     5161 ttaaagatac atataattta tagaggagga ataattatta tgattattac tattgttggt
     5221 ggaggaagca catttactcc aggtattgtt aagtcaattg cattaagaga aaaagagtta
     5281 gagcttgagg aaattcgctt atttgacatt gataaagaac gccaagataa agtagctgta
     5341 gttgtcaact ggatactaaa agaagatgtt aaaacagatg ttaaactaac tgtaactaat
     5401 tgtgaaaaag aagcgtatac agatgctaca tttatatttg cacaaatgcg tgttggtaaa
     5461 tatgaaatgc gtgagcagga tgaaaaaatt ccattgaaat atggttgtgt aggtcaagaa
     5521 acttgtggtt gtggagggat ggcatacgga ctgcgtacaa tacaaccaat gataaaggtt
     5581 attgatgatg tagagaaata tgctaaaaaa gattattgga tattaaatta ctctaatcct
     5641 gctgcaatcg tttctgaagc ttgtcgtaag ttaagaccaa atgcaaggat tataaatatc
     5701 tgtgatatgc caatagctat tatagatatg atagcggggt cattaaatat caatgacgtt
     5761 cataatatac gctatgatta ttttggtcta aatcattttg gctggtttac ttcaatagat
     5821 tataaacata gagacttaat ggaagagatt aaggaataca taaaagaaaa taaaatatta
     5881 ttaccaaaat cttatataga aaacttaaat aaaagtcgta gtacaggaaa tcgccatgct
     5941 gtaagtagct ggatacatgt ttgggaatct gtttatacaa tgttagaaag cttcccaaaa
     6001 tatctgccaa acacttattt aacatactat cttttaagta aagaaacagt ggaacattca
     6061 aacataaatc gtactcgtgc aaatgaggtt atggaaagcc gtgaaaaaga cttatttgaa
     6121 ggtgtgagac attacttaga aactggagaa atttctgaaa aagcatttta tgcaggttca
     6181 catggagatt ggattagtga tttggcagtt tcaattaaaa atgatacacg tagtcgtttt
     6241 ttagttataa ctgaaaatcg tggagctata ccaaacatgc catatgatgc aatggtagaa
     6301 attccagcat atattggtaa agatggtcca gaagttattg ctcgaggtcc aatacctcta
     6361 tttcaacaag gattaatgat gcaacaactt aacagtgaaa aattgatagt agaagcttat
     6421 atagaaaata gttatgaaaa agctttacaa gcattcacat taaataaaac tattccaagt
     6481 atgaatgttg ctaaagaaat tttggatgaa atgatagaag ttaataagga atactggcca
     6541 gaattaagat agtaggataa gggaaagggt ttttatttaa taattagata tgttttcaga
     6601 gtcttttatc aaaatgtatt cacaaacata tggcaatata ttaaaataaa gagtgatatt
     6661 tttagaagaa tggggtgatg caatgaacat tataaataaa attttttggg aagaagatag
     6721 gattggcatg attacaaata atttagaggc agatacacat agccactgta tgttacaact
     6781 gttcttaggg atagaagaca gcattgaaat aacagtcaat gaaaaattag ttaaatgtaa
     6841 ttgtatcata gtagataaaa atatctctca ttcattttca gcaagaaaaa aagtttatta
     6901 ctctgctatt attgaaccta catctatata tgcagaacag cttacctcaa aaatgaatga
     6961 ttttggctac tggatatgtg acaatgatgg attagagaaa ttacgtcaac aaggaacttt
     7021 tttgatagat aactctagta aagaacaata tttaagattt atggaaatgc ttaataacta
     7081 tttgaatata cctataactc tcaaacatta tgatgacaga attactgaat tgctcaattt
     7141 attacgtact tgtaattgtg ataatcatac tatttcaagt tttgcagata aagtgtccct
     7201 atcagcaagt agattatcac atctgtttaa agaacaaata gggatacctt taaaaagcta
     7261 tatcttattt catcaaatgg agtgtgcttt tagagaatta ctttctggga aaaatgttac
     7321 cgaagcttct atgactgctg gattcgatac tccatcacac tttgcaggga cagttaagcg
     7381 aatgatggga atgcctgttt ctttatcttt gaaagatagc gaatttttga aagtatatta
     7441 aatattctcg ttatataatg aagttgtact aagaaagggg ctttgttatg aataaagaat
     7501 atttagagga aacaaaaatg ttaaattata atgaaccaca acttaagctt ttggtatcat
     7561 caaagaattg gttggaatta gatgattttc ataaaataaa atctatttat gagtttgtac
     7621 agaatgatat tttatttgga tataatgctt ctgatatgtt aagcgcaaca caggtattaa
     7681 atgatggtat gggacaatgc aatacaaaag ctactctttt aatggcatta ttacgtgcag
     7741 tgaatatccc ttgtaggctt catgcttttg atgtaacaaa agattttcaa cgaggagcta
     7801 catccaaatt gatttcacta ttagcaccaa aatatattct tcatacttgg gtagaggtat
     7861 tttatcaaga taggtggatt gctcttgagg gtgtcataac tgataaaaaa tatttagaag
     7921 caattcagaa gaaatttttt aatcatggtg gaacatttaa aaagtatgca atagctacaa
     7981 atgatttgaa aaatacatct attgattggg atggtaaaga tacttttatc caaaaagaag
     8041 ctatcgtgta tgattatggt atttttccat caccagacgt atttttttct actcattcac
     8101 aacatatgtc aaaattaaaa aatttcatat atgtacatct tatcagaaaa ataatgacaa
     8161 aaaatgtatg taaggctaga aacaattata ttgataaaaa cgaatgagtg tttaagaggt
     8221 aactatatag gaaatatata gaataattaa aactgtactg gttaaaatga ataaaaagga
     8281 gttatcacag agcaaactta aattgttcat aggataactc cttttctgta ttaacttcta
     8341 ataatttgat gtctttttat aaaacatata gatacaatta aaccagtaaa tgcaagagta
     8401 gctgctattg aaaatgcaac atctattcca tgaatgttag acagcatgtt tgaactcata
     8461 tgagctttct tagtagcact tgtcattata gtaataagta tagcagaacc tatagctcca
     8521 gatatctgac ggagtgtgtt attaattgct gtggcatgag atataagttc tctatctaat
     8581 gttttaattc cccatgtagt taatggcatt aaaaccattg aaataccaat catacgaata
     8641 caatacatca atgacacatg aattaaagat gtatctctgc ctaaaaaagc gaaggataaa
     8701 gttcctaaaa acaaacatcc tgttcctaat attgatagag catgaggtcc atatttatct
     8761 aaatgacgtc ctgcaattgg attgagtaca accatcaata tagcaccagg taacattaat
     8821 gaacctgaag ataatgcaga aaatcctctt acagattgaa cgtacagagg aatcattaat
     8881 gtagcagaca tcattgaggc atatacaatc ataattaata ttgtagaaat aagaaatggt
     8941 ttatttttaa aaaccctcaa ttctaaaaaa ggttctttag attttaattg acgcaatgta
     9001 aataaaatta atgacataat tcctattaat attggtaaat atgtggcaat attagtccac
     9061 ccataattac cttgattagt aaatccaatt aataaaccac caaaacctag agatgatagt
     9121 actattgaag gtatatctaa ttttagtttt tgagtttccc caacatttct taatagaata
     9181 aatgatagta taacatctaa tatagcaata ggggaaagaa acagaaataa actatgccaa
     9241 ccaaatgaat ctactaacca tcctgataaa gttggaccta ttgctggagc aaatcctact
     9301 gtaataccaa ctaaactcat agctgcacca cgtttttcct cagggcataa atagagtata
     9361 ataacttgta aaagtggcat tagagaacca gaacccattg cctgcaacac acgagcaatc
     9421 aacatagttg aaaaattatt agaaaaaagt gcaataatac atcctataga aaagatagac
     9481 attgatgtaa taaataactt tctagtacta aatcgattta ttaaataagc tgttgtgggt
     9541 acaataatac ctgcaataag tagataaatt gttgttaacc actgaccaat atcagcagta
     9601 attttaaaat ctgacattat attaggtaat gctgttgaca atagtgtttg actgagagaa
     9661 cttataaaag caccagtcat tacaattgct attattaaat ttcctttttt attgttactt
     9721 tgggtttcca taatttataa cgtctccttt tttaattgat ttttaatgct gttcaataaa
     9781 agtacttttg gtcttaaagt gtcttctttt tctaaatatg caatcattgc atccataatt
     9841 aaagaatcat attgttgatt ttttccttcg acgtataatt tttttattgc ttctagtaat
     9901 ttaggatttt caaggagaga agtatcagtt ttgattagtt cttctatatc ttgtatcgtt
     9961 ttaaaaaata gcgatttatt atgaagggaa aagtaaactt catcttcttg tttatgtaaa
    10021 aacattgatt tttcaattaa aggatttaga ttatccatat tttctacaac taaatctaga
    10081 atttgtatag taaatttggc aataaagtat acatttattt ctcttttttg tacatgaata
    10141 atgtatataa actgatgaat tagagcatcc aataagaaaa ctaaatcaaa tataattttt
    10201 tctattttat caccatagtt atcaaataag cttagtttgt attcattcaa taagttagct
    10261 cttgattttg tttgcaattt acttgtagcc ttacgttgct cttcagaaaa gttttctctt
    10321 atataggtat ttatggtagt tctagaaaaa atatttttcc atacagcaat tatctttctg
    10381 tttaactttt ctgatgatgg aatgtcatta ttttcattta tggaatgtat aatttctaaa
    10441 aacttttcat cataataatt gattatttcg cagataaaac tttccttagt ttcaaaatgc
    10501 ttgtaaaaag tagctttaga cattttacac tcttttacaa catccgatat tgaagtattt
    10561 tgaaaacctt tttggtcaaa aattttttgt gcaatagtta taatgagcct ttgtttttct
    10621 gtcactttat tatctccttt cacgcaaaaa cgtttagtac tatatagtac ctacaagttt
    10681 tgataaatat attatacatc atttggaaaa aaaatcaata tatagactgt aatatgaaat
    10741 ttttgaaaaa tttgttgtta tttttagtcg tataaaaaag aaacttaaga gtttcttaag
    10801 aattgtttaa agaagttgca ataaattagt ggtatgatta tagtatgaaa gagaatgtct
    10861 tgaaattttt ctgtatcaaa ttccaattat ggaggtgtag tatatgaaaa agaaaatttg
    10921 tgtagcaata atttttgctc tatttatagt ttttgtagaa atgtttattg atagctctag
    10981 cgaaggaagt aaaattatga cagctgaatt aagtaaatac aggaaaggcg taaatgtatt
    11041 atcaataaac aatattatag tagcgaataa aaaatatagt ttgcccaaag attattctcc
    11101 tcaggaaagc agtgaagcga gagatgcttt ttataaaatg aataaagatg ctcaaaagtc
    11161 aggattaaat ttaaaagctt ttagtacata tagaagttat gaatatcaag atagattatt
    11221 taagtcatat gtaaaagaac atggagaaaa agaagcaaat agattttcag ctaaacctgg
    11281 agagagtgag catcaaactg gacttgcttt tgatattgga ggagatgacc agtcttgttg
    11341 ggcaaataaa aagttcaaca atacaaaaga agctaagtgg ttgtatgaaa atgcatataa
    11401 atatggtttt atacttagat atccagaagg taaagagaat ataacaggat atatgtatga
    11461 atcttggcac tatagatatg ttggaactga acatagcaaa aactttgcaa tgaataattt
    11521 gactttagaa gaatatttac atataaatta agagatggtt tgttaaaatt aggttaaaat
    11581 aaaaaataac ctagactatt agctgtgtaa agtgatataa tgtaaataca atttaagata
    11641 actactcgaa ggggagtagc ttacacgaaa gtggtaatta atcgtcaaga cagtagaaat
    11701 actcggttaa ttagcaaggg attccttgat agcaagacct tcaaaataag cattaggtat
    11761 gtttgttttg gggtcttttt ttattcaagg aatttttatt taaaaaaatt taaatttcaa
    11821 gtatacataa gagcccccaa acataaaata aatttattga agaggagggc aacaaatgtc
    11881 ttacaaaagt acttatcaag aagtaattaa aaatttagac tcaagtttat ctggtttgag
    11941 taaagaaaga gcagaagagt tgttagaaaa aaatggagct aatgaattaa aagaagcaga
    12001 taaggttcct acttataagc ttttcctaga aagttttaaa gacccattag taataattct
    12061 attgatagct gctttagtac aaatattttt aggtgaaaca gtggagtcta taataatttt
    12121 tgcagttatt attataaact ctgttctggg tgttgtacaa acaaaaaaag cagaaagttc
    12181 attagaaagt ttgaaaaatt tatctgcacc aaacgcaaag gtaataagag acaataaaaa
    12241 aatgacaatt ccagcaaaaa atctagtagt tggagatatt gtattcttag aagctggcga
    12301 ttacatacca gcagatggaa ggcttataga agcacaatct ctcaaggttg tagaagggat
    12361 gttaactgga gaatcagaac cagttttaaa gcatatagaa aaaataagtg aagatgtaag
    12421 tttaggtgac cagaaaaata tggtatttag tggtgctgta gttgtttatg gtagaggttc
    12481 ttttgtagtg actggaacag gtatgaatac tgagatgggt aaaatagctg ggcttttaga
    12541 aagtgctgaa aataaactta caccattgca acaaaaaata gaggatttta gtaaaaaact
    12601 tggtgtaggt attgtaatac ttgctctttt aatatttata attcaagtag caagaaatta
    12661 ctttatggct ggaaatggag aaatgacaca aattatttta gattcattca tgttctctgt
    12721 agcagtagca gtagcagcta taccagaagc tctatcatca atagtcacta tagtattggc
    12781 tgttggaaca aatagtatgg caaaaaaaca agccataata agaaaattac cagcagtaga
    12841 aactttaggt tcaacaagtg taatatgtac tgataaaaca ggtactctta cacaaaataa
    12901 aatgacagta gttgattatt atatgtatgg aactaataat gatgagttag ataaagaaaa
    12961 agtaaactat tcttatcatg ctaaattatt gactatggtt tctacattgt gtaatgactc
    13021 tcatataact agtgatggta aagaagttgg tgacccaaca gaagtggctt taataaatta
    13081 ttcaagtaaa aatgatttag attatgataa attaagaagt aagtatatca gaataaatga
    13141 aataccattt gactcagaca gaaagcttat gtctactgta aatagtatat atggagatta
    13201 tataatgttt acaaaaggtg ctccagatat tgtgtttagt agatgtaaat atgctttgaa
    13261 aaatggttca aaagtagata taacagatga gataatagaa gaatataaac ataaaaatga
    13321 agagttctca aatagagcac ttagagtatt agcttttgca ataaaagatg tcccagaaga
    13381 agattttata ccttcaattg atgacgaaca tgacatgaca ttggttggaa taatggcaat
    13441 gatagaccca ccaagagaag aagtaatgga tgcagtaaaa gaggcaaaga gtgctggtat
    13501 aaaaactgtt atgataactg gtgaccataa aacaacagca gcagcaattg caaaagagat
    13561 tggtataatg gaagaaggag acttggctct tactggaaaa gagcttgatg ctttgagtga
    13621 agaacaacta tatgaaaaac tagaaaatat aagtgtatat gccagagtct cgccggaaaa
    13681 taaaattcgt atagtaaaag catggcaaca taagaataat ataacagcaa tgactggtga
    13741 tggtgttaat gatgcacctg cattaaaaca agcagatata ggaatcggta tgggaagtgg
    13801 tactgatgta gcaaaagatg cttctgctat ggtattggtt gatgataact ttgcaagtat
    13861 agtaaatgct gttgaagttg gtagaacagt atacagtaat ataataaaat caattactta
    13921 tctatttgca ggaaacttag gagctatagt atccatagta tttgcagtgt ttgcaggttg
    13981 gtcaaatcca tttacagctt tacaactttt gtttataaac ttagtaaatg actctttacc
    14041 agcaattgca ttagggtttg aaaagtcaga aaaaaatata atgaataaac ctcctagaca
    14101 cccggatgaa agtatactag caggaggaac attgaaggca gttgctataa gaggttgtat
    14161 aataggtgta gttacaataa ttgcacaata tataggatta caagtatctc cagaacttgg
    14221 agtagcaatg gcattttcaa cattgatatt agctagaata atgcaaacat tacctgcacg
    14281 ttctaataat caaacagtaa taaaattagg ttttttcagc aataaatacg ttataggagc
    14341 agttttagtc tgtcttgggc tttactcttt aacactaata ccagtgctaa gaggaatatt
    14401 ctctatacca acaacgtttg gattgtacca gttggggatt tgcctagggt tagcattaat
    14461 ttcatcaata ttaatggaac ttaacaaatt aataaataga agtgaagtaa aataatcaaa
    14521 aaattaaagg gcatattgat tttaacaata tgccttttat aaatagtcta atttattcta
    14581 tattatcgat tatttggaat gcttttataa cttcaggtaa ttctttttct tcaggcattc
    14641 taaatcttat aaaattttta tcaagtccag taaaatcact tccagatata actcttattt
    14701 ttaatttagc aaactcttgt tgtaaatcta tgtccttgtt tttatgttca actgtcataa
    14761 ttgatacagt atcactagtt tcagctatat taaggttttt ccatggaatt aataattgat
    14821 tttttatatc ttttgttttt tcttttaatt cttctataaa ttgaacatca tctaataaat
    14881 ttgctgcaat actacgagat atttcagaca cttcgtaagg agtagaaatc tttttaatag
    14941 ggctaacaag ttgttctgga agaactgcat atcctgctct tagtcctgct aaaccaaatc
    15001 cttttgaaaa tgtttttaaa gcaattacat tatcataatt atttagaagc ttgacagctg
    15061 aattttcttt tggcatgtat tcaccataag cttcatctac cataactgct atatcatatt
    15121 tagctgcttc ccttactata ttttcaattg atgataaagg tataatttgt cctgtaggat
    15181 tgtttggatt gtctatgtaa attactttat attctggatt tattttttca ataaattctt
    15241 tttcattaaa cttgaaatta tcctctttct ttaaaagaac ataatcataa gttgcaccat
    15301 gcatttttat atctgtctca tattctgaga actgaggtga atatcctaaa actttatctc
    15361 ccttttcaat aaatagtcta tttaaaaggt aaataacatg tatagaacca tctgccaaac
    15421 aaattctgtc agtatctaat gctataaaat ctttccaata atcgactata ttatctttta
    15481 aagcaattga atgaggatat cctcttatca tatcaaattt taaagcatta aaagcttcta
    15541 cagctttgtt tgagtaagac acggtattta taccttcacc acagtctatc tcgaatacat
    15601 cttctgaatg tccagattct attgcataac tttttttcaa ctgcatttca atagctttgt
    15661 taatttttag tttcatactt tatcactcct tatttagtca ataataaatg ttataaaagt
    15721 tgctagattt aaaatattat gaaaatatat aatagtaaaa atgaatataa atattataat
    15781 agctcacttt atttaatatg attatatatc atatattgta aaatttctag cattataaga
    15841 ctataaattg aaaatattca gaaaaatact acaagaaacg atttattttg ctcttaaaat
    15901 aagttaaatg aatgttatta attttgtaaa ttgaaatagt ttaagtataa agttatgata
    15961 taatctaata agggtaattc tttagattat attaaggaaa tataggtata caataaaaag
    16021 ttatggagtt ggattatggg atataaatta attgtaacag atatggatgg aactttatta
    16081 ggcaataatc ataaagttac agatgaaaat aaaactgcac tacaaaaagt tataaaaagc
    16141 ggtattaatg taactttagc aacaggaaga gccttcgatt cagctaaatg caatgtagat
    16201 tttcttaaag aggacatgcc aataatagct tgtaatggtt cattaataag agagcaaaat
    16261 ggaaatataa tatattcaaa taagattgat acaagaacat gtttaaacat tttagatgta
    16321 ttagataaat acgatattta ctaccaatgt aatagtatag attctatgct ttcgaaaaaa
    16381 atagatggac gtgaagatag attaagtgta tttttaggct ctgaaacaga agtaattgta
    16441 aaagatgatt tgagagaaga aatctttaaa aaagatatac taaaatttgt aatcatagaa
    16501 gaaaaaaatc catctatttt ggatgaaata agaaaagaat tgagaaaagt tcaaggaata
    16561 aagataacct cttcatggcc aaacaatatt gaagtcatga atgaaggtgt tgataaaggt
    16621 aatgcagtta aaattttagc tgaaaaaatg aatatagaca gagaagatat aattgcattt
    16681 ggagacaatt acaatgacat agaaatgata aagtttgctg gtcttggggt agctatggga
    16741 aatgcagaag aattgattaa acaagaagct gattatgtta cagatacaaa tcaagacagt
    16801 ggagttgcaa aggcaattta taaattccta gagttaaaag aatcacttag ctcttaaaac
    16861 agtatataat aggaaaaaat aggttgacta ttttgtagat agtaattata atacaaatag
    16921 ataaaatata aaaatttaga ttcagtgaaa aagagagtag atatagagaa aatctcagcg
    16981 aattggggga tggtgtgagc ctaatgataa cactttatat tgaaaagaac tttggagaat
    17041 actacttgaa attcagtatg gtagtaggta atctcgcctt aagagaatag agtgggtaaa
    17101 cattgtttac caactagagt ggtaacacgg aatatttcgt ctcttggtct ttttgaccaa
    17161 gagatttttt agtttcattg agttacaaga cttataatta aaatacaaaa aattaaaata
    17221 tgaggtgaaa gttatgaaat tatggggtgg acgctttaga aaagcggaaa atcaacttat
    17281 ggaagaattt aataaatctt ttggctatga ttgtgtactg tacaaaaaag atatcgaagg
    17341 aagtgtagca catgttcata tgcaagtaaa atgtggactt ttaacagaag aagaaggaaa
    17401 gtctataaca gaaggtttaa aagggatact tgaagatgta gaaaatggaa agttagtatt
    17461 ggatggagag tatgaagata tacatagttt tactgagata aatttaattc aaagaattgg
    17521 agatgttggc aagaagcttc atacagcacg tagtagaaat gaccaagttg cagttgatat
    17581 gagattgtat gctaaagaaa aagcaaatga tttggtggat ttaatatctg aatttaaagc
    17641 tactataaaa gatgttgctg acaaaaaccc agtgatgatg ccaggctata ctcatcttca
    17701 aagggcacaa gtagtgacat ttaagcatca cttaatggca tattatagta tgtttgatag
    17761 agatgagaaa agaataaaaa atgctataga aatattagat gaatcacctt taggctgtgg
    17821 agctttagca ggaactacgc atgatataga tagaagtata acttgtgaac aattaggatt
    17881 taaaaaagta gtagataatt ttatggatgg agtaagtgat agagattatt tactagagct
    17941 aatgagtgat ttttcaatta taatgatgca cttaagtaga ttaagtgaag agttgatact
    18001 ttggagttct caagaatttg gatttgttga gatagatgat ttatatacaa ctggaagtag
    18061 tataatgcct cagaagaaaa atccagatgg tgcagagctt ataagaggta agactggtag
    18121 agtatatgga aacttatttg gtctttttac agtaatgaaa ggaataccat tagcatataa
    18181 taaagacatg caagaagata aagaaggttt tttcgatagt gtacatactc ttgaaatgtg
    18241 tatacaaata atggatagaa tgatagccac actaaaggta aatgaggata aaatgaaaca
    18301 agcagtaaaa aatggttttt taaatgcaac agaagttgcg gattatttgg ttaaaaataa
    18361 tgttgctttt agagatgcac atggtatagt aggaagtatc gttatatatt gtgaagataa
    18421 taaaaaagca atagaagatt taacattaga agaattacaa aagttttcag atgcttttaa
    18481 ggaagatatt tatgatttta tcgactatga gagtatatta aataagggaa taaaaaagaa
    18541 tttaaaataa tgtatttatt tttaaaagct gtaaatttga atgttatcat ttaaatttac
    18601 agctttttat tgtgtaagat tttattatat aaaagtaaga aacttaaaag gagtagtatt
    18661 taaaataaat acaaataaaa aattattatt aaaatgataa ttaatatcaa taagtattga
    18721 caaaattatt tctaaatact attatatata atatatatgt attaaaatta agacaaaatt
    18781 ttaactttac tatttgtgta aaaagtaata taatttaaaa ttgattataa atctaacttt
    18841 tttctttatc aaaatatttt aggaggtgag acatttgaaa aaaactaata gtttaaaaga
    18901 aattttaaac tggaataata tcaagaattt aagaccttca aagggtttaa aaaatatatt
    18961 agaaattttt aaaagtgacc taaaagatat acgtggcaat tatgccgtac ttgtaatagt
    19021 tttaggtctt gcttttttac catctctgta tgcttggttc aatataaagg catcttggga
    19081 cccttatgga agcactgcta atatgcttgt agcagtaaca aatgaagata aaggaaatga
    19141 aatagcagga caaaagatta atgtaggaga ccaattaatt gaacaactca aagataacaa
    19201 gagtttaggt tggcagtttg tagatgaaaa aactgctctt gaaggtgtta aaaaaggtac
    19261 ttattatgca tcacttaaga ttccaaaaaa ttttacaaaa gatttgacat ctttaattac
    19321 agatgatgtt aaaaaaggcg aaattatcta tactgttaat gaaaaaataa atgcagttgc
    19381 accaaagatt acagataaag gtgcttctac aatacaacta caggttaatc agactatagt
    19441 aaaaacagtt agtgaaatcg ttcttggtat atcaaacgga gttggtgcag gaattgaaca
    19501 cagtcttcca aaattgacaa aagttgaaaa ttcccttatt gaccttcaaa agagatttga
    19561 ccatataaat aagactgttg atttagcttc tgatgctact tcaaaattat ctgatacagt
    19621 taaagatatt aaatctgatt tacctacaat taaaaagact ctgaatgata caaaactatt
    19681 atcaagtgac ttaaaaaaat tcttagagga tacaaatgat aatttagacg aactttctcc
    19741 tcttattaag tctgacttaa atttaatggt tgacctttca tcttctgctt cttctcttac
    19801 attaaattta attgatgcag taaatagtgg cagtgaagat gtacctaagt tgatagataa
    19861 tttatcagaa aaactttcta atttgcaatc tttaaacgat actttggtgg actttttaac
    19921 taaattaaac caacttacat caaataatag attggatgat gtaatagata atcttgaaga
    19981 ctctagcaat aaaatagatt catctatatc aacattaaat gatattaaaa acaaagttat
    20041 atctggacaa caaccatcta taagtgcttt gaataatgtt ttaagtctat caaatggtat
    20101 tggaagaatt aatttaaata ttttaaataa ttttgactca aaaatatcaa aacctataaa
    20161 tagtatattt gcaaacagta taaaagtagc aaatgatata attactgttt tagataaggc
    20221 agaagcaaaa cttccaaagg ttgaagaaat acttactact tctctaaaac tttcaggaaa
    20281 tgcacaggaa agtatatcat tgataagaga aagacttcct ttagcaaaag gaatgttgga
    20341 tgatttgatt gatactttaa gcaaaattag taatggtgaa gatatgaaaa aattagtcag
    20401 cttgcttgaa agtgatatat tgacaaagtc taatttctta aaagaacctg ttgaggtgaa
    20461 aacagagaaa ttatatccaa ttgaaaatta tggttctgca atgacaccat tttatagtgt
    20521 tctttctaca tgggttggta tattacttat ttcagcacta ttgtctacat ctgtacatgg
    20581 aaactataaa ccttatgaaa tttactttgg aagaggcctt acttttttaa gtatagctct
    20641 tgtccaaggt tttataatag gaactggaga tatattattg ttaggagtaa cagcaaaaca
    20701 tccaatttta ttgatagttc tgcttatgtt tactagtatg gtatttaact ttatagtgta
    20761 ttctttagtt tctgtatttg gaaatgtagg aaaagcaatt gcaatagtac tattagtcgt
    20821 tcagattggt tcttctggtg gtacattccc tatacaggtt acacctacat tcttccaaag
    20881 aataaaccct ataatgccat ttacctatgc aataggtgct acaagggaag cagttggtgg
    20941 tatatatgct ccaaatctaa ctaaagatat actagttcta ttactattta tgattctgtc
    21001 tatttgttta aatgtacttc taaaaggacc tataaataga gttataaaac catttaatga
    21061 taaattcggc gatagtaatt taactggtca ttaaaaaatt aattctgtct ttaaatatat
    21121 tttttttaat aatattctaa gttcatatta aaaggtagta gctaaaaagc tactaccttt
    21181 ttccaactta aaaactaata tttactacaa aaactgtcat agaaaaaaac gaaaaaattc
    21241 gttctttcct aaatttatct tataaatatt tagatatatt cctacttaat aagcagagct
    21301 atacatagta tttaatgtat aacttacatt atttaactca tataatcaat tattcctgaa
    21361 tttaaattat ataaatgctc aaaaccttct tcagctaaca aattacatgc agtaacactt
    21421 ctatgaccac ttctgcaata aactactact ttcttatctt tataatcgta aatttcatct
    21481 atattattga ttaactcttg taaagatata tttatagcat tttctatttt atattcctta
    21541 aattcatctt tagttcttac atctaataat aaaacatcct tttcattttt catcatttct
    21601 ttaagttgct ttttatttat attcttatac attgtctaaa attcctccat tttatttata
    21661 tattacctat accctttatt ctttatacaa caacaaatat tatttttata tttttctctg
    21721 ttatataaaa agctatactt ataaaagctt tttttattga taataatctg ttgattaaaa
    21781 taattaagaa tgttatgcta attataaatt attaaaaagg gaaagaggta atgtaatatg
    21841 tttaagcaaa ggctctctaa attattatct agtacattag tattgagtat gttatttact
    21901 gctgctccaa atattacttt tgctgataat acaaaagata attccgaaaa atatcaaagt
    21961 tctgatatag aattacatga ttatagtaaa aacgcggaat cttacacaaa aactaaagct
    22021 cttgctaaag agaaaatcca aactttgcta tcaaagtatg gtgctgtaag tgctcaatat
    22081 gctttgattg ataatggcaa aattgagatt tctggtaatg gtggagttta cagcaaacag
    22141 gataacaaaa acttaaataa ggataatatg tatagtatcg catctatcag taaaatgttt
    22201 actacaacag ctgttatgaa gttggttgat gatggaaaat taaatttaga tactcctgtt
    22261 gtaaagtata tacctgaatt taaaatggct gatgatagat acaaggaaat cactccaaga
    22321 atgttattaa atcattcatc agggttgatg ggaagtagtt ttaaaaacac tattttatta
    22381 gcagacaatg attcttatgg tcatgataat ttcttaaaag aattacaaaa acaaagatta
    22441 aaagctaaac caggagcttt ttccgtttat tgtaatgatg gatttacttt agctgaaatt
    22501 ttagtagaaa gagtatctgg tatgtcattt actaattttc ttgataaata cataaataat
    22561 ccacttaact tacaaaatac aaaaactaca gaaaatagtt ttgatagttc aaaactagca
    22621 aaagcatacg tgccttattg ggaagatgca gttccgcaag ataaccttaa tgctataggt
    22681 gcaggtggtt tatattcaag tgcagaaaat ctatgtacat ttgcacaaac atttatgaaa
    22741 aattcaaatg gtattctttc tcctgcttca gtaaaagcta tggaaaacaa agaatattta
    22801 aatggtctat ggcctgaagg agaagactct atacttggat atggtttagg gtgggattgt
    22861 gtcaacactt atccatttaa tcagtataat ttaaaagctc ttacaaaagg tggagactct
    22921 ttattattcc acagtaatct tatagttctt ccagatgaaa atatggcagt tgcagttctt
    22981 tcttctggtg gaagtagtca acttaatgaa ataattggac aagaaatctt actatctgca
    23041 ttaaaagaaa aaggtaaaat taaagaaata aaacctgata aaacatttag taaaccacaa
    23101 caagttaaaa tgcctagctc tctaaaagaa aatagtggtc tttatgcttc ttcaaatatg
    23161 ataaaagtag atgttaatga taatggtact ttaacagtat cttctcctta tatagaaaat
    23221 ggacctgaag ataaatacgt atacataggt caagatagat ttgtatctga aaaaggtaac
    23281 agctgtctaa aatttgtaaa agaaaaaaac aatatcacat atttaaatat gagctcttat
    23341 gatgatgtcc ctggattagg tcagacagca agtttatact atgtagctca aaagatagat
    23401 gacaacaata tatcaaatag tgtaaaagaa gcatggaaaa aaagaaatgg taaagattac
    23461 tatctagtag atgaaaaata tacttctcaa agctatatgt ttggttctgt gaaggcaact
    23521 cttgctcttt ctgatgaaac tccaggttat atagttaaca ctaaaataat ggatgagaat
    23581 aactcaaatg cttttataga aatacctggt gtaataggtc gtgatttatc cgacattaaa
    23641 cttcataaag aaaatggtac agagtattta tcttttggaa cactaacgta tgttagtgaa
    23701 gattctataa ctaatttgcc agcagaaaaa tcttttactt gtgaactaga atccaatggt
    23761 tatgcaaaat ggtataagat tggtgacgat atcgcaaata aaaaaataga agttaatctt
    23821 cctcaaaatt cagcttttgc tgtttatgat gataaaggtg ttcctgtaaa ctattcttta
    23881 gttacaaaga acaatagagt tagattgcca aaaggaggcg taattgtttt cttaggaagt
    23941 ccaaatgcta gatttgaggt tacatatcaa gatgaagtaa atgcaagtgc acttacagga
    24001 actgatagat atgaaacttc tattaagata agtcaagcag gttgggaaaa tgctgaaaat
    24061 gcagttttaa taaacgattc agcaattgca gatgctttgg ctgcaacacc atttgcatac
    24121 aagaaaaacg ctcctatatt attgactgga agctctcaaa taaatgaaaa aactttggct
    24181 gaattaaaga gattaaaagt taaaaatgta tatgtagttg gtggagaagc ttctataaat
    24241 gaaaaatctt tagatactat aaaatcaaac aatatctctg tatctagaat ttcaggaagt
    24301 gatagatatc aaacttctat gaacattgct aaagaattga acaatattag caatatatca
    24361 aagatttcag tagttaatgg cgaaaaagga ttagccgatg cagtaagcat tggagctgta
    24421 tcagctcaaa atgatatgcc tataatactt actaatgaaa atagtaatat aactgaaata
    24481 aataacttgt ttaaaaacaa aaagattgat aaatcttatg taatcggtgg agaatataca
    24541 gtttctaaaa atatagaaag taaacttcaa aatccacaga gaatatcagg tagcactaga
    24601 aatgaaacta atgcaaaagt aataaaagaa ttttacaagg attctaagat agacaattta
    24661 tatgttgcta aaaatggaat gaataaacaa gatgatttaa tagacggatt atctgttgga
    24721 gttttggctg gaaaaactaa atctcctgta atgctagtag gaaactcact agattataat
    24781 caaaaagaac tatttaaaac tatgagattt aaatctgtaa cacaaattgg tggaaatgga
    24841 aatgaaaact catttaagca aataaaagaa atagcttaac ataactaaaa aaggctgtat
    24901 tcaagtaata ttaattgaat atagcctttt tttatttaag ttataagttt tatatgtgta
    24961 aaaattaaat ttaaatatta aatgataagt ttattaagat atagataagt ctaaaacttt
    25021 gcctttttta agttagtttc tatttatcat tcatacttaa caatttatct aaatatactg
    25081 actttaattg tttactggct aaaatctttt tagctggttc aagctttaat atagctgaca
    25141 gtatagctcc catagctcta tgactagcca gagcattatt atcaaaaatt aaattttgta
    25201 actgcccttt ttcaattgcc ataagaactg ctctatgtac tgaatttgct ggagttatta
    25261 ttttttcatc tctctttaat aacataatgc tatttttatg gcaatttcta acacaaactc
    25321 cacaacctaa acatctatcc tcatcaattt ttatatattc tttaccatct tccttaacct
    25381 tgcttatcgc atctattgga catgcagtta tacatttacc acactttaca caattttcat
    25441 ggtttatatt tggtataaaa cttgttgtct gtactggatg aagatttcca aatcttttag
    25501 ctgctaccat tgcttcacag caacatccac agcaattaca tataaacgta acccctttcc
    25561 taacattttc tccacattga actaagttat gttcatatgc ttgatgaagt agctctttac
    25621 attctatttt atccacacgt cttgcaaatt tattatttat taaagaattt gctacattgt
    25681 caaacgtcat acatatatcc ataggagcat cacaagcttt tccaacatgt tgcattctat
    25741 gtctacagta acacatactt atacctatat gagttgattc atcaattata tgagtagccc
    25801 tttcataatc taaaatacta acctcattat cattagttaa aacttcctct tgaacgtata
    25861 ctcttccaag cttcgtttct gttgaataaa ataaatcttt gataaaatct tcttctacat
    25921 tcatatattg atagtaaagc tctgccaaaa gtttttggtc tatatcgtgt ctcgttctca
    25981 tcatggcaaa ctcaaaaaaa ccagccattg gaggaggcat tatatatttc ttccctttat
    26041 tatcttctat atctagtatc agagccttac tagcaagttt atccaatact ctatatgctt
    26101 cactttcact tacactccaa attttagctg ctgtttttac tctgaaaggc tttattggaa
    26161 gctgagcaac taacttcgcc tcttgttctg tatatagtac atttagtatt ttgtataatg
    26221 tatcagatgg tggagcacct tgtggaaact tgtttattct ttcctctaaa cttttataca
    26281 tattcttcgc agttatatga gaaatagtaa tcaccttctt tttcttttgt tttccaataa
    26341 aatcaaatat tctttcttat tatatattct cttactttat ataataccaa ttctttctaa
    26401 atagttaaat acctctagta atttattatt agatttctta tataaataaa aaattattta
    26461 agtgatataa taatatttta taccacttaa atgattttaa taatatttta tatagttaag
    26521 aattattttt atatctatat actttctctt cttatattat ctattatatt ttctttattt
    26581 aatttactca ttggtatata ccctgctata aaagttatta taaaaacact tactatacag
    26641 ataactatgg ctgtccacgg tataatcgga gaaaattcaa ttacacctga aataagttta
    26701 ttcataatta gaattatacc aatactaatt ggtattccaa acaacaatga cagtatacca
    26761 tatataaaac tttctaaata tatcattttc ctaaatcctt taggagtaac ccctatagat
    26821 tgtattactg caagttctct ttttcttaga tttatacttg tacttactgt atttagaata
    26881 tttgtaacac tcactaaaga tatcactact ataaaaccat atacaaatat tttaataacc
    26941 tttaaatctt gtttttcact ttcattgctt gcactttcat cataaaagtt aaaattattt
    27001 ttatttgcca aagcttctat ttccttctta gtcttaatgc ttcctcctga atcaataaat
    27061 ataaggcttt tacttaattt taaatttaaa tttttagcaa cttcatcata agttataaat
    27121 tgtaacccca tattttgata accaagacct agacgttctt cgtcagttat agcaagaatc
    27181 ttgaccttta cttttttact taaggcattt tcatcaagat attcacaatt tactgtatct
    27241 cctactttat agttggtaag ttctactaca cctttcttcc cattttcttc aattgcactt
    27301 tttctaacta atataacacc attttcttca attgctctct ctttattaaa agagccctgt
    27361 actaagttat ttattttgtt ttttactgca aaatctcctg gaaaaattaa ttcattatta
    27421 ctgaaatcat attcataact atctccaatc ttatattttt gataatattt cctgattaaa
    27481 tcttccttgg atttatttat tttattttca cttactctta tagacacata ttgtcctgtt
    27541 gccaattcaa agttttttat cccatcaata tcttctagtt cacttatagt atcctctaca
    27601 gacctagctg tgttctttgc agttaaatac aaatcatatt ttctttgaga atttctaatt
    27661 tcttcacctc ttagtaaaag taatgtaaag ccactaaaag atataaatat tattatactt
    27721 accattaaag aaaaaagagt tattataaat ttttttctat ttctcctaat atttttatat
    27781 gcaagtactc cttcagtttt aaaaatcatt ttaataagtc ttgagctttt gattttgccc
    27841 actttatact ctccagtatt tttaatcaca ttaaggggag aaatattaga agccgatatt
    27901 gcaggtagta gaattgatat aaagattgta agcaaaacta ttattatact aacaataatt
    27961 attattggat tatacactat ctgcaaactc atcttagtaa caaccgattc agtaaaatac
    28021 ttatttataa ttttaaacaa taagtctata gctactgttc cagatattag tcctattggt
    28081 ataccaatta aactaattat aataccttct ataaaaacta atcttttaat ttgactagat
    28141 gtagcaccta tagaatttaa tatcccaaac tgtttttttc gttcatttat agaaatacta
    28201 aaggaattgt acactgtagc aattgtacat acgattacta aaatagttac tgctataatt
    28261 atagacttta tagagctatt tatatttgaa tacttactag caccttgtaa cctcaataaa
    28321 tgttcattgt attttatcat atcagaaact gcttctttat ctttatttga atatatattt
    28381 ttacttattt tattacttat cttatacaca tctttaggat tttttactgc tactgataca
    28441 ttaattgtat cattgtaatt attcatagga ttaaagtagg ctatagctgt tgcaattcca
    28501 ttataacgtt caaacccagg tttttcaatt ataccaacaa ctttaaaagt tctatctttt
    28561 tcatttgcta tataatcacc ttcatgaaat gtcatactat caatcttttt cttgcttgag
    28621 tcaaacatat cccctacttt taaagttatc ttatctccta tttttaactt atcatctata
    28681 agattaataa tactttcact taaaacaatt tctccttcct tagctggaaa tctaccttct
    28741 tttatagaaa ctttatatcc atttattgca tttttatcat attgctttat acttaatata
    28801 gcattttcac tattttctaa tttagaatat ccaagttgtt tggtaaatgc atgtttttct
    28861 atttcagctg atttcgttac atattccaca ttttttctat ttacatcata aaatgtaaca
    28921 tggaaacttc catcattctt tatagtttct ctcatttgat aatccataaa actttcaaat
    28981 atatttccta taccacaaat taaagctgtt gaaagtatta taccgattat ggttacaatg
    29041 gttcttcttt tattttgttt taagtatcta atagttagtg atgtatataa attcatctac
    29101 atcacctcat cactttttat gattccatct tctatgctta taattctatc agcttgaagt
    29161 gccatatttt tgtcatgagt tatcattatt agtgtctggt tgtatttttt tactgacaat
    29221 tttaacaatt ctaatatttc ctttgaattt ttactatcta aattacctgt tggctcatct
    29281 gctaatatta tagaaggtct attaattaat gcacgtccta tagaggttct ctgttgttga
    29341 cctcctgata cttggtttgg aaggtgcttt tctctatcaa ttagacctaa tgttttcatt
    29401 aaatcatcaa aatattctct atcaatatct ctattgtcaa gctctgctgg aagcaatatg
    29461 ttttccttca catttaagac tggaattaag ttgtaaaatt gatatataag acctatattt
    29521 cttcttctaa atatcgccaa atccttagtt tttaaattat atatatcaac atcatttata
    29581 taaaccttac cactagttgg tctgtcaact cctcctaata aatgtaataa ggtactttta
    29641 ccacttccac ttggacctgt tattgctata aacgttcctt tttcaactga taaacttaca
    29701 ttttttatag catcaacttt tgtttcattt ttaccataac ttttagttag attttctact
    29761 cttaaaattt ccataactac ctccaatatt tatattctat acttcatata ttaggttttg
    29821 gctattacta taaagtgaat taaatattac agattagtca ctatcataca ttttatgaaa
    29881 tattatatga aattctgtac ccttaccttt ttcgctgttt acatatatat cgccattttg
    29941 gctttctatt atagattttg acattgcaag acctattcct acactatctt cttttgaact
    30001 acttcttcct ctatagaatc gtttaaatac atgtggtata tcttttttat ctataccttc
    30061 accctcatct tttattatta gttcagaaaa taatggattt tctttatagg ttataattat
    30121 atttccaaat tcaggagtat gttctacaca atttttgatt atattcacta atgcttccac
    30181 ggaccaatca atatcaccaa tataagatac attatcattt ccctctatag tcagtttttg
    30241 atttttaatc tccataggta ttttcataga tagcattgct ctatgtatga gttcactaaa
    30301 ttttacctta tcttttttaa aatttatcac ttttgcttct acctttgata atttaagcat
    30361 gcttttaatc aaccaatcca ttctatttaa ttgatttttt atcttattta aaaaatcgat
    30421 ttttacttcg tatggtacat cattatataa taaatcattc aacattatta aagaagtcat
    30481 aggagttttt aattgatgtg atatatcgga aattgtattg tttaaaaata tcttttctct
    30541 ttttaaaagt tcaacctttt cattaagaat tgtcgtcatt tttaagagtt cagtttttag
    30601 aagacctatc tgcccttctt gattcttgtt tttcatatca aatggcttac cctctgaact
    30661 atagtaaaca tacttagtca tatctgataa atcattgtaa attgacgtaa aatatctaac
    30721 aatcaacaca aatatcaaaa taaatattat acatactaat attatattta ttttgatagt
    30781 ctctaaaact aatttagaaa taataggctc agagtttatc ctaatagatt tatcataatt
    30841 atacttaatt aatattttct gaccataatc tatgttctcc atagacttcc cttgagttat
    30901 tatgtctact atctgacttt ctaagtttgg atacttgctt gataaagttc ctattatagc
    30961 ctgattattt cttacaacca tctctttagt taaggacacg ttgattatgc taaatccaat
    31021 agatattatg ataacaataa acatcaaagt aacatactta cttaaaaact tcttcacttc
    31081 tggattatat agaaacacta ttttcacttc ctatccattt ataaccaatc ccccttacag
    31141 tttctatgta gattggttca cataaatcat cacctatttt ttctctaagc ctttttatat
    31201 aaacagttag agtattgtcg tttacaaaat cataagttac atcccaaagt ttttccaata
    31261 tttgagttct acttaaaaca gtatttttat tctgtataag aattaaaaga agtttatatt
    31321 caactgatgt cagaaatatt tctttatcat ttttatatac tctagcttct aatgtatata
    31381 tacttaaatc tccaaattta agtattttct tattttcttc tgaagtatta ccttttcttc
    31441 ttaaaacagc ttttattctg gatattaatt ctctaattct aaaaggtttt gttatataat
    31501 catctccacc tatatcgaga cccattacaa tatttacttc ttcatcacaa gcagtcaaga
    31561 atattatagg aacctgagag aatcctctta tatattgaca aagctcataa ccagttccat
    31621 ctggcaatgt aacatccaac agtatcatgc tatattcatt agaagaaatc atttcttttc
    31681 cactagataa atcctttgat atatctatac taaatccttc ttgttctaaa gcatacttta
    31741 ttccaaaaga tatagtacta tcatcttcaa ctatcaacaa ctttaacata actatttcct
    31801 ttcttttcat tttaatttta aactatctgc ttaatttcat actatctatc tatacaagtt
    31861 ttattataca caattaccct ggatactaaa atatttctaa aattacaaaa tagtcacaaa
    31921 gaactattaa aatttataaa aataaaatgt aagttgttac ttaactatct aaagaatgat
    31981 actgattctt tcaagttaag ttacaactta catattaaaa ctagatatta aataatttga
    32041 cattactatt tgttcagtta tacaaaatat tacttattct tcttcatatt ttttgtattc
    32101 atcttctttg taaatattat cttttttagc atcttcatgt aaatcaagct cttcctttac
    32161 atctttaaca ctatttacat attgcatcat ttcttcttga gaaacatgtg gacaattttc
    32221 acaatttatt cttcttgaat taaattcttt tacttttttt tttaattctt catattcata
    32281 atcttgtttt gtgtctctac aatcacagtc ataatcaaat tcgtcatatt tataatcatc
    32341 ataatcgcta tttccataat aatttgtatt gaccatttga gaattagatt tatggctttt
    32401 taccatttta tatacagcat atgctcctac acctgtaatt acagctcctg taatcatcat
    32461 tggtttactc attccatcat cttttttatg catttgtctt ttcattaaat atgttaacat
    32521 attttttctc ctttctaatt tttatttcta tctataaata gtatctgtat tattttaata
    32581 aaaattatgt tattttttaa attacaattt ttacaaaata taaacttata ttttgtgttt
    32641 gttattattt tttatgttat actatgtata taacaatcgt tttcatttat aaagcataat
    32701 ggaggttttt tatggtttta aatttagatt ttaatagtga taaatctatt tatgtacaaa
    32761 ttaaagaaga aataactaag gctatagctt caggagaatt gaaaataaat gaatcacttc
    32821 catctgtaag aagtatggct gaaaatattg gtgtaaattt acatacagtg aacaaatcct
    32881 acaatgaatt aaaagaagag ggattcttaa atattgatag aagaaaaggt gctattgtag
    32941 ctcaactacc aatgaaaata gataatacaa ctcgtcaaaa aaataaactt gacttggaat
    33001 tattggtggc aaaatcttat ttatcaggta ttagtaaaga agaatttttg tcactaagtt
    33061 caagtttatt tgacaagtat gaggtgaaat attatgagta agattgaatg tctatttaca
    33121 atatttccag ttctaatttt gatgtatgct acattttatt ttatacccta ttttgtaggt
    33181 aaaaaacaca tctatggagt ttctatagac caagagcata aaaattataa ttactttata
    33241 aagttagata aagaatttaa aaaactatta tctttaggtt ttattataga ctttatatta
    33301 gtttttattc tggtctttac atttaataaa ttggaacttt cctattttat tggtattata
    33361 ggttttttat tatatgaaag tatactatat attcatacac ataaaaaagc taagaagctt
    33421 aaatcagaaa tttatgctaa attaggtcat atagatatag attcaaaatt aatagtagat
    33481 atggatttta taaataagaa aaataaaata ataaaaaaat ttaaaataat ctatttaata
    33541 ccgatactat ttactttggg aatgagtatt tttatagcat ttaattataa tcaattgcca
    33601 gactcaattg ccactcattg gaacataaat gggtctcctg atgtttttat agataaatca
    33661 tttcttaatg tatttaaatt aatcggtact gacttttgtt taatggtttt attatatata
    33721 acttccattg gatccataaa gtcaaggatt aagatagata caaatagaat tgaagaaagt
    33781 agagttgaaa acattaaata tttaaataaa attggttatt tgtttttaat attgatgatt
    33841 ataatgacaa cacagttttt tactactctt ttatcaataa aaacaaaact attaactgct
    33901 atgaatatta caacgctttt agttataatt tatttaatag caacatatat taattcccca
    33961 aatttaaaat tcaactcatc ttattctcca gaagaagatg agaagtattg gatagcaggc
    34021 atcatgtata ataatccaaa tgacccatca ttaatggtta ataaaaggtt tggtattgga
    34081 tggactataa attttggcaa tcctcttgga aaaatactat atattgctat agctttactt
    34141 ttaatttttt cattgtttag cctaataaaa tctctcttac tttaaaatac cataaaaggt
    34201 atcttacttg tattattggg tattatattt tgtaggataa gaaatatttc atattatcgt
    34261 tgaagtatag tatttatata ttactaagaa tggagatttt cataatgttg tcaaaagctg
    34321 aaatcaaata ttttaatgaa tgtgcttctg agatactttc ttctgaaaaa gttcaactta
    34381 tgagaacatt tcctcatcat ggcaatgttt catgtcttga acatagtttg tcagtagcat
    34441 attatagtta tctattatgt aaaaaattgc acttaagtgt tgatattcaa agtgttatta
    34501 gaggagcttt actacacgac ttttttcttt atgattggca ttacaaaggt gatagaaaag
    34561 gattacatgg atttactcac cctagagaag cattaaaaaa tgcaacttta ttttttcaaa
    34621 taaatgaaaa agaaacggac ataattttaa aacacatgtg gccacttact gttaagccac
    34681 ctagatacaa agaagcattt attgtatgtt tgttggataa attttgctgt ttagttgaga
    34741 ctttaaagat acattcttta ttatctcctt accatgtata ggacaattcc cacaacactt
    34801 ttataactta attttgaaga ggtattaagt attatattca acttgatacc tctttactat
    34861 ttattatttt atataataat attttcttta taatatcgtt ataaacaaca acttctaccc
    34921 tcgataactt ccccttctta tagacatgct tactacctta aataacaaga aaccagctat
    34981 taaatttatt agtattattt gtactactct aacaataata tcataaataa acctccaaat
    35041 agcttctctt tcactacctt ttatcttaaa ctatattttt ttcttaaaat ttgtaaaaaa
    35101 catagcataa tctttataaa tgttctaaaa tttaatttaa gcatcaaaat tagaaaggat
    35161 gatattatgt ttaaagaaat gagattgaaa aaaagagaaa tgacaaaaga agatactgtt
    35221 gaagtattaa aaaatggtga atttggtact ttttccacta tatctgaaaa tggttatcct
    35281 tatggagttg ctgtaaatta tgtatacttt aatgattcta tttactttca ttgtgcaaga
    35341 aatgggcata agttagataa tatatcgaaa aacaacaaag tttctttttt agtagttgct
    35401 aatgaaagtg ttattccaga taaatttagt actacttatt ctagtgctat agtttttgga
    35461 aaagcttgca ctgtagaaaa tgaagaaaag aaaaatgctc ttgtagaaat aataaaaaaa
    35521 tattctaaag gattctttga agaaggcatg aagtacatag agaaggatat gaacttaaca
    35581 actgttgtta aaatagaaat tgaccatatt tctggtaaag cttcacgttt ataattaagt
    35641 aaaaatacct aaaagtatat acagtcgctt ttaggtattt ttatatgata ttcttatatg
    35701 ttaagtcttt ttaggtaatc tcttatacct atcttggcat catcatttgg ccttatatca
    35761 gaattatctc ctgctatatg aagtattcca tacgctggat aacctcctct tactgaacat
    35821 ggaactgaca aaagagtcat tcccatatgt tgggcaaata tattcatagc ttgcaaagct
    35881 aattctcctc ctccatgttg tgaacctgct gtagcaaaac aaccaaatat tttaccactc
    35941 aaaggagcac ctacccaaat atcagaaaaa ctatccataa acatcttcat tggtccagat
    36001 acatttccat aatatgttgg tgaacccata aatatagcat cataatctaa tatttctttt
    36061 ccagatttaa ttacatttat actttcaaat tcgtctttgt attctttgga atttatcaaa
    36121 taatattgag gaagtgtttt tgcaacttcg tcacttactc taaatatatc tacctcgata
    36181 ttcatttctt caagcgcttc tttatattct ctagctaata aatatgtact cccacaaaca
    36241 ctatgaaaaa ttactgctac tttcatcatt atccccctaa ttaaactcaa atttattttt
    36301 tatatagtat ttttattatc aaacactgct aaatagtact aaatggattc aaatatacat
    36361 tactctttat tacttcaatt tcactaaatt catctaaaaa tctcttcata acatttttga
    36421 atatatcttc tgtatcaaaa tgaccacaat ccactataca cattccaata tcaagtgcat
    36481 cttgtgcctc atgatacttt acatcacccg taattagtac ttctgcacct tgtctcttag
    36541 ctttttttac aaattctgaa ccagctcctg ttacaacggc tactttcgtt atgtctgtac
    36601 taaggtttcc aactactctt atgtgtttca tatttaattt ctcttttatt ttccttgaaa
    36661 gactttctaa tgtcatactt ttatcaagtt tacttattct acctagtcca aatatctcac
    36721 ctttattttc taacttgtac aaatcatatg caacttcttc ataaggatgt gcatctaaca
    36781 tagaacttat taccccacct aaaagcttct gtggtactac agtttcaatt ttaacttcat
    36841 ttacagtttc tatatcattt acacttccaa gaaacggatt agagccttct aatggcttaa
    36901 attgtccttc accttctgtt gaaaacgtac aatcactata attccctata tgacctgctc
    36961 ctgaattact taaaaccttt ctaagttcat ctgaatagtt atgtggaacg tatactgcta
    37021 atttatacaa attttcgctt ttagttatat ctaaaacctt tgaattacca aattccataa
    37081 tttccataaa atagtcattt agaccatcaa atgctatatc aaaattcgta tgcatagaat
    37141 aaagtgatat atcattttta ataagttttt ggataagtct gccttttaaa tctccactat
    37201 taattttatt aattttccca aatataaaag gatgatgtgt cacaattaaa tctatatttt
    37261 tggatatagc ttcatcaata acatcttcat tagcttctaa agataccaat acctttttta
    37321 catccatatc aaaatctcca actataagac ctacattatc ccaatcctct gctaagttta
    37381 gaggatactt tttttcaatt tttctagtta gactctttaa tagcatatat acctcctatt
    37441 tattttatta aattttcaag tttttttatt gcaacttcgc tttcctttct cttcttatct
    37501 atagcttccc catttttccc ttctaattta ttaactatag aactatatgt ctttatcttt
    37561 ttttctataa aatcattaaa aagagaatct ttgttttcta aaagctttat tccaacctca
    37621 taatatatct catcttctat tatagtattt ttacctgtat atttggcaac aattatctca
    37681 tatattctaa aatcttctct cactaaaact tcttctaaaa tttcatatcc gttgtttaaa
    37741 aggtaatatc taagttcttc ttgtgcttgc attggctgta atattaattt ttctacatta
    37801 tgtgctactt cttttttagc ttcgagaagc tcacttatca agattccacc cataccagca
    37861 attatcactt cttctacttc ccctatttca agtatctcta taccagaacc caatcgtaaa
    37921 tcaactttat ctaaaagatt gttctgtatg acttctttat gtgcattgtc aagaggtcct
    37981 ttattaacat ccgctagcac tgcaaatggt acttttccct cttttaacaa atatactggt
    38041 atgtatccgt ggtccgttcc tatatcagct atttttttac catctgacac taatgatgct
    38101 attttcaata atctatctgt taatttcaaa tataattctc ctttcaattc cctttattta
    38161 taggatttaa tccaagttat agatattaga ctgaaataac tacaaaaata aaattaaatc
    38221 tttactacaa aatagccatg agatatcact tatctcatgg catttatcac aaaaacatca
    38281 atgatgtatc tttttagtct aaataatctc taagtttttt tgatctactt ggatgtctta
    38341 gttttcttag tgcttttgct tctatctgtc ttattctctc tctagttaca tcaaactctt
    38401 ttccaacttc ctcaagagtt ctggctctac catcctcaag accaaatcta agctttaata
    38461 ctttttgttc tctatcattt aatgaaccaa gtacatcttc tatttgttct tttaataaag
    38521 aatatgctgc tgcctctgct ggggctggag catcatcatc tggaataaag tcacctaaat
    38581 ggctatcttc ttcttctcct attggtgttt ctaaagacac agggtcttga gctattttca
    38641 ttatttctct tactttatct tctgtcattt ccatttcttt tgcaatttct tctggttttg
    38701 gatctcttcc aagttcttga agtagttgtc ttgatactct tattagctta tttatagtct
    38761 ctaccatatg aactggtatt cttatagttc tagcttggtc tgcaatagca cgtgttatag
    38821 cttgtcttat ccaccatgtt gcataagtac taaacttata tccttttgta tagtcaaatt
    38881 tttcgactgc ttttataaga cctaaatttc cctcttgtat taaatccaag aaaagcatac
    38941 ctcttcctac atatcttttt gctatactta caactagtct taagtttgct tcaactaacc
    39001 tttgttttgc tatctcatca ccttcgtgca tccttctagc aaattctact tcttcatgtg
    39061 gtttaagtag aggaattttt cctatttctt ttaagtacat tcttacaggg tcatctatac
    39121 ttatcccttt tggtaaagat aaatccacag tttctatttc tattgcagaa gagtcatcat
    39181 gtgaaattgc ctctgcatct tcatctaagt ggcctatact taaatcgtca tcagcaaccg
    39241 aaaaatctat atcagcttta tagttttttg tttctgttat ttctattccc aaatttccta
    39301 aagtctcata aagattttct acttgatcct tatcaagttc agtctccgaa aaagcttcca
    39361 ttatctctgc aagtgtcaga gaaccttgct tttttccttt ttctattaat gtcttagcag
    39421 taaccttttt taactctttt ttattcgatt tattttccac actcaaacct cctttctcta
    39481 caagctcttt aaaattttat ttatctcaac tatttttaaa gcaatttcca taactctacc
    39541 atctacctct ttcatatcat tattattttc taattcttgt tgttctctca acaagctatt
    39601 aatttgctct tctaatgagt tttttctgat atttgttatt attccttcta tttcttttgt
    39661 gttctctaag tttatactat tcaaagaaat tgaatttaat tcttttaagt attcttcgga
    39721 tatgtttaaa cttttcaatt tgtcaatagt tattttgtcc aattcttgat ttttaatcat
    39781 gtaatttaaa atttctttac tttctttcaa taaaaaatca ctttcttcta ctttaagtaa
    39841 cgctatttct ctaatttttt tgtcttctaa cattattttt attagagttt cttcaactaa
    39901 ttgttttccg tcttgtctga cttcgacttt ttcaattact ttttcttcta ttttcttttg
    39961 atttttgtta ttatatggct tattgtaatt tttaccataa acttctctct ttatagactc
    40021 gacattaata tctatttgat tgcttaaata ctttgtataa aaatcaattt caacaggact
    40081 tgtcaattgt tttataattt tagaagcttc ttttgcgaac ttcactcgtt cttcatcttt
    40141 ttgaatgtta aattcttttt taagatgatc tattttatat tttatgtagt gagtagaaac
    40201 atccatagct tttttataat cactaagtcc gtattttctt acaaattcat ctgggtcttt
    40261 agcatctttt aagtctaaca cttttacatt gatacctaat ttagtaagta tatctatagc
    40321 tcttaaagtc gcttttattc ctgcttcatc agaatcataa gatataatag cagtgtccgc
    40381 atatctcttt attaaaatgc cttgttgttc agtcaaggct gttccaaggg ttgctactac
    40441 attttttata ccatactgat aaagtgaaat taaatccata tatccttcaa ctaagactat
    40501 agttttactt tctaaatttt ttctagcaaa atttaatccg tataagttct gttttttatt
    40561 aaaaattaat gtgtctggag aatttaagta ttttggcaaa gaatcgtcta atactcttcc
    40621 cccaaatcct attatatttc ctctataatc gaatatagga aacataactc tatttctaaa
    40681 tttatcatag caattagttc catctctatt ttttgcaatt agaccgcact ctaataagtc
    40741 ttgtttttta tatcctttac tgattagagt attcataagt gaattccatg aatccaaaga
    40801 aaatcctaaa ccaaatttct taataatttt atcatctagt cctcttattc ttaaatattc
    40861 atacccaaga tttttgcttc ccaataagtt tgaaagataa aatctagctg cttccgtatg
    40921 aatatcttga aatttcttag atttttctat tttaatccta gtttcttcat tcatgttagt
    40981 gttaatttct ataccgcatt tatttgctaa aatttttaca gcatccataa aatccaaatt
    41041 ttccattttc atgacaaaat ttattacgtc tccaccttca ccacaaccaa aacacttata
    41101 aatctgtttt gatgtattta tataaaaaga aggtgtcttt tcgccatgaa atggacataa
    41161 tcctttgtaa ttagctccag attgttttat attcatatat tcagatatta tacttgctat
    41221 atcgcatcta gcttttattt cttcaataat gtcttttata tcattcatat tatcaccttc
    41281 tggtgtaatt ttttgtgtta acatttctat tctacactat attctaaatt tgtcaatcac
    41341 tcaatataca caattcgact ttttttattg atatccttct tattaatcaa aaaattttta
    41401 ttttttatat taattctatc aatattaaat ctttatttat attaaataat ctaactatca
    41461 aattataact tataaattta ccttagcaac gattacgaat aaaaaagagg ctcttgcctc
    41521 ttttaataat tattaattga tttcattaaa tcttcaaatt cattaaagca aagagattga
    41581 gcaccatctg acaaagcatt ttctgggtca ggatgtactt ctaccattat tccatctgct
    41641 ccacatgcaa aagatgccag tgacataggt tttaccaaat atctaactcc agtggcatgg
    41701 cttgggtcta ccacaacagg aagacctgtt tccttttgaa taatgggtac cgcagctaaa
    41761 tctaatgtat ttctagtata atcattatac gttcttatac ctctctcaca cattataata
    41821 ttactatttc cttctattgc tatatattca gaagcaccta tccattcagt aattgtggat
    41881 gctattcctc tttttaacat aacaggttta ttttgttttc ccacttcact taaaagagta
    41941 aagttttgca tatttcttga cccaatttga attatatcac ttatactata cagttcgtcc
    42001 atatctctta catccataag ttctgtaata acagccatac caacttcgtc tttaactgct
    42061 tttaatattt caagaccttc tttttttaat ccttgaaatg aatttggcga tgttcttggc
    42121 ttgtatgctc cacctcttaa aatgtttgct ccttgacttt ttacaaattt agcagtctcc
    42181 aaaagttgct cataactctc tattgcgcat ggtccagcta ttataagttt atcttttttt
    42241 acagttatct ttttgttatt tgtatttatc tctatctttg aattattatt aaataggttt
    42301 tctttcataa cctacaagct cctttacttc ttctctttct tcagcagata ggaggtttaa
    42361 atgttctttt attgtttttt catttattat aagttcttca tttaaatcca taagtggtat
    42421 caaaacgaaa gctctatctt gaatccttgg atgaggtatt gttagtcttt catcagtgga
    42481 aactacatca ttaaaaaata gtacgtctac atctatagtt cttggtcccc aatgaactat
    42541 tctctctctg tgtagttctc tctctatttc ttgacaatat tctaacaact cataaggttc
    42601 aaactctgtt tctatttcta cacacatatt tagaaaatct gcttgttctg tatatcccca
    42661 tggttttgtt tcgtataagc ttgattcttt tactttatat atagaatcac tatttttcaa
    42721 aagttcacat gctcttgata aattatcaaa tctatcaccc atgttagtac ctattcccaa
    42781 ataagcctta ttcatctctt gttctcctta tctcaacacc aaaatagtca taaataccat
    42841 ttacaggagc ctcaggcttt ctaactctga ccataactcc atttataagt ataaatttat
    42901 tcaagacttc ttcagcaata ttttccgcta aagcctccaa aagattaaat tgtttatttt
    42961 cagtaatatc tttaacaaca ttgtaaacct cggcatagct tacagattta tttatatcgt
    43021 cactaagacc tgcttctctt gaatcaatat aaagttccat gtcgacaaag aatttctgac
    43081 ctagaaaatt ttcttcttta agaacaccat gatatccata gaatcctaga ttactaagta
    43141 atattttatc cattatatta tacctacttt ctatatattg catcagtcat tctagctgcc
    43201 attaaatttt cataaacatc atgtactcta actatatcta cgccatctct tataccaaga
    43261 acagtagtag ctattgtacc ttctaatctt tttttaggct caactggaag tacttttcct
    43321 attactgatt ttctggatgt cccaagcaaa ataggatacc ctaaatcttt taattcattt
    43381 aatcttctca atacttcaat attttgttca aaatccttgc caaatcctac acctgggtca
    43441 agtactattt tttctttttt tacaccacaa ctcattgcca tttctatact taccttaaaa
    43501 aagtctttta ttgactccat tatatctttg tcatagtttg tcccatcttg attatgcatt
    43561 atacacactt ctgcatcata ttttcctatt acagaagcca tatttttatc ttttctaaga
    43621 ccccaaacat catttaccat agtgacacct aattttaatg cttcttctgc aacatctgcc
    43681 ttataagtgt ctattgatat tggtatatcc aattcctgtt taagtttttt tattacaggg
    43741 attactcttc ttagctcttc gtctgagtct acatagctat gacctgggcg tgtagactct
    43801 cctcctaggt ctattatatc tgcaccttgg tcaaccatat cttttgcatg ttgtattgct
    43861 atgtccaagt tattaaaatc tcctccatca gaaaaactat ctggagttac atttaatatt
    43921 cccattatat aagttctttt accataatca aacacaatta atgacactcc cttaaattaa
    43981 attttatctc aataaatgat tatatctata ataaattgct acttcatagt aatcaaactc
    44041 atagcctcgg ctctagacgc aatgtccttt tcaaatattc ctctcactgc tgatgtaaca
    44101 gtttttgaac ctggtttttt tacgcctctc attgtcatgc acatatgttc agcttctact
    44161 actactaaga caccataagg ttgtaattct tccattatta tatctgcagc atttttagtg
    44221 attctttctt gtaattgtgg tctttttgcc aatgtatcta ttactctagc aagtttagaa
    44281 agccctgtaa gtcttcctcc ttttggtaaa taagcaatat gagcttttcc aaagaaagga
    44341 actagatgat gctcacaaca tgaataaaat ggaatatctt ttaccaatac taactcttca
    44401 tgtttttcat ctgcaaaaag aacttttaaa tggtctcttg gttcttcact aagaccagag
    44461 aatatctctt catacatcct agcaactcta tttggagttt cgattagacc ttctctatct
    44521 gggtcttccc caattgcctc tagtatttct cttactgcat gttgtatctt ttctttatca
    44581 actttattca tttaaactcc ccccattgat ttgtgactag aagattagtt ttataacctc
    44641 ttttgtctca atttctttaa aaatataatt ataaacatct aaaagtcttt gatagtcata
    44701 attttcactt acttttgaca ctagttcaaa aagctcatta ttttcttttt ttaaaacttt
    44761 tagcaatttt ttgtcttttg gtacccattt atcatttata ttaaagtacc caattattat
    44821 atcttttaaa tataaattcg tcaaaaaatg atattcaaaa acatcaaatt tttctttatt
    44881 ttttaaattc gatatttttg ctccaatatc ttgctttata aaagactttt cctctaatga
    44941 cattttactg ggtccttcta agtaaatatt tctacttata tcttttataa tatgtgatat
    45001 attctcccta tcaaacagta ctttagcatc tttcatttct ttcacaaaga aatattccct
    45061 atttgataat aattttttta caccatcttt tgaaaagtaa tttacatcaa attctattcc
    45121 ctttttttta aacagtattc tctcttgttt ttccccttct tttacaaaaa cgaacacatc
    45181 aatatcgttt atctctacat cataaagagt aaaatctacg tctttactag aacccactaa
    45241 atatacagcc aaagtgtttt ggttttctat tattttagtt atctcttcat tgtatacttc
    45301 ataaatacaa cctatcatct tttgtctcca acaaattgtt aatattttaa tttattctaa
    45361 taacttcact attttatcaa aaacaacatt ttctttatta aattttattt tgtcaatttc
    45421 tgtaaccttt atagcgccca taagactatt tgtgacaaaa acaaaatcaa aactactaat
    45481 ttctgatata ttaatttcat tttctatcaa ctctatatgt aattcgtcac aaatctctat
    45541 aatccttttt tttattattc catttaatat cggtaatcta tcactaggag tgtacactct
    45601 ttctcctttt acaaagaaaa tattgctcat agaacattct aatattacac cttctgaatt
    45661 taaaaatata ccatcattat aaccagtctt atttgcgata tttttggtat atatgttttc
    45721 aaaataatta gtagtcttat gtctatgaat taaactattc cccctaacta ttggagatat
    45781 agctaattta aaccccttct catacgtctc ttgattatat ggaatatccc taatagatat
    45841 attataacct tcatcaaaaa cagtaattct aagtgcttta ttaatgacat tttctttttt
    45901 tatatatgta acaatttcat ttatcaagaa atctttttta tattttatat ctaaatccaa
    45961 acaatttatt gaacttagca ttctttctat atggatatct aaatctactg ctattccatt
    46021 tttaactcta atagtttcaa acaaaccaat tccaaattta cttaaactac tatcaaaacc
    46081 tacattattc ttcataatat cccctcaaag ccttcattat ggatttagct ttatcaagtg
    46141 tttcttgata ttcttcatct gggtcagaat cccaagtcat tcctcctcca acttgaaaat
    46201 agactttttt gtcatttttg attattgttc taattgctat attaaaatcc atatcaccat
    46261 taaaacctat ataacctatt gaaccagtat atacatttct ttgcgttggc tctaattcgt
    46321 ctataatttc cattgctcta atcttaggtg ctcctgttat agacccccct ggaaaagttg
    46381 cttttattac atcagtagca tctttatcat cttttaactc acctacaact gtggaaacta
    46441 agtgatttac atttgcatat ggttcaatta caaatagctc tggaacttta acacttccag
    46501 ttttagatat tctcccaata tcatttcttt ctaaatctac tatcataagt agttcagctc
    46561 tatctttttc actatttcta agttcttgtt gaagtcttaa atcttcttct ttatctttcc
    46621 ctcttggacg agtacctttt attggtctag tttctattct cttattaaca catcttataa
    46681 atctttctgg agaatttgat aatatatgtg catcttcaaa gtttaagaat gccccaaatg
    46741 gtgctgggct aaatcttctt aagtccctat acaattcaaa gcttgtaagt tctgtttctc
    46801 cactgaacct ttgtgttaaa ttagcttgat atatatctcc ttgtcttatg tactctctaa
    46861 cactttgaac tgcattttta aattcttcct ttgtaaaatt agatttaagt cttataggtg
    46921 ttacttcttt ttcttcataa catatgctat ctataccttt tttctcttct tttaatattc
    46981 tttgttctat atcatctatt atcttttctt ctaattcgat atctatgttt ggagtagcta
    47041 tataagtttt ttgcacaaga tggtctataa ctattacatg attataaaat ccaaaataca
    47101 tatcaggcat ctcaatatca tctacagcag ttctaggtaa attttctatg taattaccta
    47161 aatcataaga taaatatccc acagcacctc ctacaaatgg aagattagta tcatttacaa
    47221 ccctatactt atgtagttct tcttttaaaa cttctaaagg attttcatca gtatctttat
    47281 actttaatac cttaaatggt tggctactta taaaagaata tcttcccaat ttttctttgt
    47341 ccatagcact gtctaatata aaactgtcat gttcatttct aaaaatagta aatatctcaa
    47401 aagaatttaa tttagtattt atctctctta tcatattaca tatgtaccct ttctctagct
    47461 tctgttataa aatttttaag catttcatga ccatactctg acaaaatagc ttctggatga
    47521 aactgtactc cttctattaa atatttctta tgcctaatcc ccattatttc ccctttatca
    47581 gtaattgctg taatttctaa ttcttttgga actgtattgg agtcaactat tagtgaatga
    47641 tacctagtga catttaaagg atttttaagt cccctaaaaa ctcctttatt agtatggttg
    47701 atactatata cttttccatg cacaggctgt tgtgctttta taatatctcc tccaaaaaca
    47761 tgtccaatag tttggtgacc taaacaaatt cctagtatag gttttttccc cttaaaatgc
    47821 tcaacaatat ctatgcaaat accagcttct tttggagaac acggtcctgg agataacaca
    47881 attacctctg gatttagttc ttcaatgtca cttattttga tttcattatt tctttttact
    47941 acaactgttt cccctaattc ttctatatat tggactaggt tgtatacaaa agaatcataa
    48001 ttgtctatca taagaatcat actgtaaatc tcccccctaa aaattatttt attaactcag
    48061 aaactgttac aaatttaaaa cctttttcaa tcatttttgg aataaacata tctaaagctt
    48121 caactgtatc attagttgca taatcatgca aaagtattat atctccattt tgtactttat
    48181 taattatttt atctgcaatg ctattagctc ctggattttt ccaatctttc acatctacac
    48241 ctgtccataa aacaacttta tagtcatgct tttttgcaat ttctattaat tctttttctc
    48301 tataacttcc aaatggtggt ctaaataatg tcggtttttt tccagttact tctttcaaaa
    48361 tgtcttcaca cttaacaatc tcctcttcta tctgactaga acttaaatta cttatatcag
    48421 gatgattgaa agtatgattt cctatctcgt gaccttcttt acttgctctt actaatggtt
    48481 ctttatacca ttttgcatgt tttccagcta taaaaaaagt agcttttaca ttgtatttct
    48541 ttaatacatc taaaacttga ctagtttctt taggatgagg accatcatca aaggtaattg
    48601 ctattagctt tttttccctt gaaccatgtt taatcaaaac atttccatta gtatccatca
    48661 tcaaagcaga aactttacta aaattagata ttaacactat tccacaaaac aagactactg
    48721 ctattaagca cttatatact gtcttttttt tccatgatgt cattttttaa cttcctttca
    48781 aatattttag ttatttataa aaattttgag tgtaatatgt tttatagctt cctccaaata
    48841 ttactccaac tccaatatta ttatagttcc ccaaaatatt ttttctatgt ccttcagagt
    48901 tcatccaagc ttcatgcgca tatattgcat taatctgtcc tgctgctatg ttttctccag
    48961 ctgaagtata tactatacct tctcttttca ttctgtcaaa tggagtttca ttcttcttat
    49021 ttacatggtc aaaaaaatta ttatctctca tatcttcact atgttttctt gaactcaaag
    49081 ttgcctgttc actgtatctc aatgagttta accctctttg atttctaaca ctatttacaa
    49141 ggtctataac ttcaagttca aaacattttt taagttcttc gctatcatct ggatataaat
    49201 ttttaaactc atcttcagtg cttttatcaa ttattaaata tgaacaaact ctattttctt
    49261 catgaatatc gtagaaaatt gtaatatact taccttcctt acttattata tcatattgat
    49321 tttctgagtt tattatatat ctggtatttc cttttctctt atattttaat ggtgtaatct
    49381 tagttctcac tgtttgtcta tcttgattta gttttatatc ttgattttca catgaattaa
    49441 tagaattact ataaagtgct actacttcat cattttcaag tccaaccatt gcaaattctt
    49501 ttccatattg attatataca taccaatcaa aaccatattc actaatatca attctaccag
    49561 gtttgccgat acttgataaa acatagctta aattatctcc taagcttatt ttttcaaaat
    49621 tctttatatc tgactctcct aagttctccc cttcagtatt atttgattgt aaattttgac
    49681 ttttttcttt tttatctttc ttagaattat catttatatt tattttgtct aaaaattcat
    49741 tccatatttc actttgaaaa aacttttctt ttaaatcata tccttctttt aataattcag
    49801 gactataaaa taaaacaata agtactacta acactaaagc aattaatctc ttcaatatat
    49861 ctctccttct taaaatcaat caaaattttt taatattgca tttattaagt taatattgat
    49921 agtgtatact aatcaatatc attcatacct atttacagta agtattagaa aaaagcttta
    49981 tctactaatt agataaagct acagtctcaa ggaaaatatt tcatctaatg ttctatcaaa
    50041 tatagatggc ttatcaaatt tccaagtccc ttcataaacc aattcattat ttagataaaa
    50101 agttccttgc ccatgttttt taccatgcat aaattcacct atatactttt tacctttttc
    50161 ccatatataa gttcccttac catgaatcaa attattcttc cattctcctt cataaagtac
    50221 ttccccttta ctattatagc aaataccgta cccttccatc tttccacctt taaattcacc
    50281 tgcatatata catccatcac tccacttata aatacctttt ccatcttttt ttccgttttt
    50341 ccaagttcca atatattcat ctttactact atataacata tttccaatac cgctcatcat
    50401 atcttcctca aaataaccta tatattcatt tccatttgaa aatataaagt ttccttttcc
    50461 atttttttta tcttctttaa attctcctat gtactccata actgttcctt catctacaaa
    50521 aaaagtatat gagcctttcc cctccatctt tccctctaag aatttcccta catatacatc
    50581 cccatttgaa aatttataga ttttattttt tggttttttt gtatcgacat catctatatc
    50641 gcgtattgtt tttccttcca attctaatac ttcattttct ggagcaaaat cgcccctttt
    50701 taaaacacgt ttcttcatgt tctttatact tctaatctct ttgtttactt catttatata
    50761 tttcaaataa ttatcataca aattaacata ttctttagat aaattcataa tgccattcct
    50821 ccaacgttat tcctagatat tttactaaat ctattaaatt tattatatcc taatagtggt
    50881 catgttttct acatattaag taattttttc aattttaatt ttaaaatgat atcttttaaa
    50941 gtgatacaca aatcattaat aaaaccttta ttttcgtata ctaaatttaa ataagctgag
    51001 agatataata tacaaattta cattataaac tcccagcttg taaaattttc tataaatctt
    51061 ttgctttaaa ccctcttcct tttacttctg taacatcatt tacagatata aaggcctttt
    51121 tatcatactt caaagctata tcttttatct ttggtatctc atttgatgaa actattgtat
    51181 atataatctt ctttttttga tgagtgtatg caccttctgc atctaaaaaa gttacacctc
    51241 taactaaact cttcattata tcttctgcta ttaaatcgac ttcatttgac attaccataa
    51301 cagatttttg gtaattcatt gcatctttaa taaaactcat tgaatacgca tttagaaata
    51361 gacctataag agtgtataat gctaaatcca acccaaataa aaaacttcct gtaagtacta
    51421 tcaagccatt tattgctaaa gcagtgtctt tcatttctat attcttctta atttttagta
    51481 ttgcagctat tatatctgta ccaccttgag aagacctagt tctaaataca actccgactc
    51541 ctaaaccaga taatatacca ccatatacac tttgaagcaa tatatcatgt ataggtataa
    51601 tattacttat ttcctgagtt gctcctaata ataatgaaaa tacaatcata tttaccaaac
    51661 ttgaaataca aaactctttt tcaaggtata tgaaccctaa gataaatatt ggaatattga
    51721 ttaaaaatgt taataaacct acatttatat ttgtcaaata gtttatagat gttgctatac
    51781 ctgtagcacc tccactaagt agatgtgctg gtcttaagaa cccatttatt cctatagatg
    51841 tcatcaacat cccaaatagc attgttgaat atcttaaaac tggatgtttt tgtagggtat
    51901 tcttaacttc actcatttct ttctcccttc aatctttcta aatctttaga attttaaaag
    51961 cttactctta catatattat tcctgatgta atagataata tttcaaataa atttttttta
    52021 taatttcttg aactgtcatc tattacatca tctattatat tatctttttc atatatgtct
    52081 agtgcaatct tttatattct aatctttaaa aacgttcaaa tcatctcatt tatgccttaa
    52141 atgaaacttt ttctagcata atatgatact gcttattatt taacttgcaa tatagtaaat
    52201 ttatagtatt tttttctaaa tatctatttt cgcagtaatt tattttattt tagcaaattc
    52261 aagtttagta attgtctctt tataattaat tcctaaatga aatttttctt tttataattt
    52321 cagtacatga tttttttatt tcattatttg catttttttc gttcagtttt tattttaaat
    52381 tttttttatt ttattttgtt ttaagtattc aagttactcg tcttaatttt attttttata
    52441 taaaattcat tataattgga attagtatta ctgtaattgt accagagact ccaatagaaa
    52501 caccactcat agctccttct atttctccaa gttcaaaagc ctttgtagtt cctaaagcat
    52561 gtgcagatgt tccaagagca attcctttag caactggatg atttatattc cccagtttga
    52621 atactgtaga agcaaccata ccaccaaaaa tacctgtaaa aattatggct acaactgtta
    52681 ttgattctac accatttaaa gaatttgcta aagatattcc cattggtgtt gttatagatt
    52741 ttggaattag tgaatttatt atacttacat ctgaattagc tattttgcca attatcaata
    52801 cagatacaaa agaaatcact ataccacaac ctattcctac aataatctca aacatatgct
    52861 ttttaaataa ttcaaattgt ttataaagtg gaactgcaag tactactgtg acaggaccta
    52921 aaaagaaatt tagactatca gctcctatct tgtaattctc ataagggatt ttagatatgc
    52981 ttaaaaacaa tattattcct acgattgcaa ttaataaagg gttacatata ggattacttg
    53041 tttttcgttg aatgtatctt cctaaattga aaaaaactat tgttactatt ataccaaata
    53101 ctggtgtctg taatacatta tacatttttt tgtccttctt ccttcttttt tatcattttc
    53161 tccacaataa ttccagtagt atacattact attagtgtac ttaaacatat gactaataaa
    53221 agcactaata cattttccct tattaggtct aatgacttta taagtgatac acctgctgga
    53281 ataaaaaata tagccatatt atcgaggaaa aatccactta cattttctat actactcaag
    53341 tctaaaactt taaattgtag tagtagaaat aataatagca taccaactat actcccagga
    53401 ataagtataa ttccttgtat aaaggaactt atatactctc ctattaccca gatagataaa
    53461 attattacca attgattgaa aactttcatt ttccacctcc aaaaattaaa gagattgctt
    53521 caaatcatat atttgtagtt ttatcaacaa gcaaatatct ttctttaaaa tagaaagtta
    53581 taatttgaca caatctcttt ttaaatttat ttttatttta atttaataaa atacatactt
    53641 ggttgtttca aaaaacttaa ctatataaac ctatttttca cttaaaattt cttttatttt
    53701 tgaattgtta tacatactgt ctggaacttc acttctcaaa ctataaaaag tctgttgtgc
    53761 attttttaaa tcgccattat cataataaag catacccaac tcgtaatatg aatcatcata
    53821 atactggtca tttttagtat aagtatttat atattttttg taatatttta tagcttcatc
    53881 cttatttcct aataatttat ttgttattgc taattgatat attgactcag aaatatattt
    53941 tttagtttta ccagaagaaa ctacactttt aaaattatct atggcttttt catattcttt
    54001 gtttttcttt aaatttagag cttgataata taaatcttcc tcatcagcta cagttatctc
    54061 tctttctggt tctttatttt taacctcatt taattcctta ttcgtttttt ctaatttttt
    54121 actcgtttct cctaactttt tatcagtttc acttaacttt tcctctttac tacttatttg
    54181 gttattcaag ttttctattc tctttttgct ttgaatcaaa tatatcccca tacctattaa
    54241 aacacagcca actataatat atggcaaaat acttttcttt ctttttactt tttttatttc
    54301 ttccttttta cttgtatctt ctttttcttc ttcatttttt tcaaatacag gattatcttt
    54361 atctagctct ttaagtttgt ctaaatatcc ttctctcttt tttacattac ctagtttatc
    54421 atataacaaa gataaaatta catatggctc tattagttca ggttcttctt caattatgtt
    54481 ctctaaaata tgtattgact cataatttaa ttcttcattt ataaatctta tagaatgatt
    54541 gtatcttcct aaaaaagttt taaaatcttc agatgaaatt agatttacat attttctaga
    54601 tatatgatta ttttcataac ataaagcagt ataaaaactc tcaaatgatt tagcaaaatc
    54661 acattttaat aattttaaaa gacctctaag atttagtata tcaatgtctt taggatttat
    54721 ctccagagct ttattaacca tattaatggc actagttata taatttttat atgctaaatc
    54781 caatgcattg ttatatcttt tatatgattc taattttaac tccttagacg ttgtatctga
    54841 ctttgaattt aaagctcctt gcaagtttaa tagagtttct tctttataat ttgtagtcac
    54901 ataactctgt tttaatgctt ttaatgtttc atcaccgtct tcttcttcta tattaaaaaa
    54961 agtcaaatca cttttattat cttctatcaa agatatatct aaaatttttc taggcatgct
    55021 ttctatgtct tgttttatat ctttaactaa aataatactc acctccactc taaaatatat
    55081 atttaagata acatatttaa tatgtcaagt caaaactata catatttttc caccattctc
    55141 ataataaaaa aggtaatcct cttcttttca taaaaaggtt acctcttttt caatactatt
    55201 aacttttaat gttgaatttt tttacttttc tatagagtgt agctatacct atatttaatg
    55261 cctcagctac ttttttcata ccttctgtat tacttccata tttctttatg gcattttcta
    55321 tagcttccct ctcaaggtca tctatagtct taacttcttg tttataaagt tcatctagtt
    55381 taaaagatat taaatcagac atactcctta caaaagcagt taactcctta tcttttgaaa
    55441 gtatcttttc tttttgttct tcattaaacg cagctatacc caagattcca agtatatgct
    55501 ctcccatttt aacgggagta catatatcag ctaactcttt acaattttct ctatttgcac
    55561 atttattaca gttctcttct atttctttat ttataacata ggatttccca tcttttaaaa
    55621 caagagagta cactgacttc tctgatattg tctctcctat tttacctctg aatattcctg
    55681 tacctgctat cctagttaaa tcttttgtaa ctaaagtaac atctacatcc aagacattag
    55741 aaatattctc gcaaatactt tgaacgtaat tcgttatatt atttaaattc aaagtaatcc
    55801 ctcctattta gatttatatt ttttattact ctacataaat cctattcttg agaactttaa
    55861 gcttgcggtt gtggttgtgt ctcattaggc ttattatcag ttggtggtgt ttctggtttt
    55921 tgttgattat ttgggttttg attgtttgta ttttgattat tcttattttc gtctgtattt
    55981 ggtttttcct ctggttttgg ttccatagtc ccaactaata taataccttc cttcttagga
    56041 taatatgaat tagctatttt ttcagtttta atttttttac catttgcatc attaaatgtt
    56101 ctatatgttg atacagaata tgcatttctt ccacttttgt ctactttttc tttaccttta
    56161 ggcattgtag ggtcattaac tcttttcatt gtagtttgtg aaacgccatc taattttgtt
    56221 gatattgtta tattttgttt atcttctgca cttccatata tattgcaaac aatttgattt
    56281 cctgaaacgt aattttttat gtaaactgga tgctttagat tatttttaaa taagaaatct
    56341 attccactat ctgcaacagt tgcatctctt ccttttggaa cataacttga tggtatagta
    56401 tgatttttta cattaacaat ttctaaacct gcgtatagca cagaattgta taacgtagat
    56461 gaaacttgac aaactcctcc accataatct tcttccatct caccttccat tattacaggt
    56521 gcacttttat atccattttc cttacttctt ttccctgtat gtttattaaa tgagaaagtt
    56581 tctcctggca taagtaatac attacttata ttatctgttg ctaattttat gtttatactt
    56641 ctatttattt gagaagtttt aaatgttgtt gaatacgtac ctaaatttgt atctatatta
    56701 gcaagttgct ctttttttac ttttgctgga gtagctttta ctactaactc atttttatag
    56761 ttaccttttt tagtttgatt atcaaaattc tccatactct tagaaatatc cattttaaga
    56821 ccatcagaat ctggtataac cttgactttt tcaccactta tatcaagtgt agcatctttt
    56881 acatctacat ctatttcttt agctatcttt tccatttctg cttttaattt atttttatca
    56941 taatttatcg caactactaa gttgcttttt ttgccaaaat ttgctgaaat agtttttgat
    57001 agattgccaa agaaagcact ctttctattt aaattatatg cgttttctac tgttttatcc
    57061 aggtcagtag ataaatttaa atctttgctc tttactttcc agcttttatc attgtaatta
    57121 aaatcaacat tctctaattt gtatttttta gctaattcct gttttgcttg tgatttagtt
    57181 aattttccaa tatttactcc attaacatat gtgttttttg ctattttctc ccctttaaat
    57241 tgcatgcaca ttatgcctat aaaaattatt actactaatg ctacagctcc aatagctata
    57301 actagctttt tgttcacatt actcatccca tacttctcct tacaataaaa ttatttatat
    57361 atccctatta ttatattact acataatgcg ccttaaaacc ccaaaaactt tttaattata
    57421 tattaattta atattattaa ttaatattca tttagtccat tttctgatat ggcatatatt
    57481 ttttcattgt aaatattaat agcataaact tctttgcctc taaataagcc tttatttact
    57541 acagcataat aataaatatt tttatatttt ccaaacaatt cttctaaact ctttttactt
    57601 tttacatatc tatctgcttt ctctatatct aatatctcaa tctttttata cttattttct
    57661 ttttcatcaa gagtattagt taacacaaac ttagctttat cttttgtaat accaaattga
    57721 taacttccat cttctaatat tctaaagtat ccatcaccat aatctacttt gtaatctttt
    57781 ttaccatctt tatatgaata taaatcttta gtttttgcac ttaccaaaac ttcatttatt
    57841 aatttatcat actcatatac ttcagcataa tagtatttat tattttttat catttgtgga
    57901 agtgaattta tagatagatt tgccttgtct ttgccacttt ttttagctat tacatttata
    57961 atgtcatcag atgttaaagt atacaatgat aaattctttt tatatttttt ttctaaactc
    58021 tgtacctttt catcatctga ataatcgtct tttaaatact caatatattg taatgctctt
    58081 tcataatctc cagaagtatt tgcctcttca gctctactta tatagtaatc atacatttct
    58141 tcaatacatt gttctttccc attttgagcc atactgtaat acttttcatc ttcttttaat
    58201 actttattat acttttcaat agcttcataa tacttatatg ctttttggtc ttttactgct
    58261 gagtcataaa catctctaga ctctttgatt gtttctatat tctgtttgta tacatctagg
    58321 ttactaccca taccatttaa tattgaaaca gtacttatgt agttaatggc aatatcataa
    58381 gtagtatttt cttctttata catttctcta actctatcag cttgagctaa taagtcatcc
    58441 acatctatac ttattttgtt taattctttc acggtattta ttaatccaat aaaactttct
    58501 tgacttatgt ctccatctaa atatttatca ccactattta atagcaattt atttattttt
    58561 ttagaaacag acttattaaa cctttccatt tttgaatcag aaaacttttt ttctatactt
    58621 gtgtagtaat caacagctat agaatactgt cttttctcta taatctttaa caactcatta
    58681 gcttcatttc ctacactaaa ctcgttacta cttaagaaaa atactattat caaagcaatt
    58741 acacctaatg ttgataatga aatcgcaact atagatttcc ttttttcatc aataaaatct
    58801 acagccttct caaataaatc tctaaaattc attatagttc cctctccatt taataatctt
    58861 tatattttat ggtaaactaa atttcttatt ggttattata tcataattct tgaaaaaaaa
    58921 taaaaacaca agttaaatcg tgtttttatt ttacctatca atcattattt tatacataaa
    58981 tatttccttt acataaatta tgatatcttt ctttttatat ccctagaatg actcttttgt
    59041 tttttattat tactgacagt tttcgttctt gtcttgtgta tagctttgct atcaaatatt
    59101 tttctagcat tgcataaaac aattccaatt gttaaacaaa ctgtaagcac acttcccatc
    59161 aaaaagtaat aatccaatcc tatatgtact cccataactg aatttattaa cactaatgca
    59221 aaagtcattg caaaacatat catcatggag ataactaaaa ttcttagaat ttttttcaat
    59281 ccactcacct cgtataatca ctaaaattga cactacccct tatccattac tataccaaat
    59341 tagtaaaaaa atagcaatta ctattgtcat ttatattagg aaaatattaa atctttttaa
    59401 taaatatctt tttttgacat aaggattact taacttttaa taaaatagta acactaatat
    59461 gtattaatta atagaataaa ataaatgata atttattgag gggtgattta catgccaaat
    59521 ttgccaagtt tagggtcaaa ggctcctgat tttaaagcca atacaacaaa tggtcctatt
    59581 agactctctg actataaggg taattggatt gttttatttt cacatcctgg tgattttaca
    59641 ccagtttgta ctacagaatt tttatgtttt gctaaatatt atgacgaatt taaaaaaaga
    59701 aatacagaac taattggtct aagtgttgat agtaacagtt cacatttagc ttggatgtat
    59761 aatatttctt tacttacagg tgtagaaatt ccatttccta ttatagaaga tagagatatg
    59821 agaattgcca agttatacgg catgatatca aaaccaatga gtgatacatc aactgttcgc
    59881 tctgtattta ttatagataa taatcaaatt ctaagaacga ttctttatta tccactaact
    59941 acaggaagaa atattccaga aatacttaga atagtagatg cacttcagac tagtgataga
    60001 gataatatag ttactcctgc aaactggttt cctggaatgc cagtgatttt accttatcct
    60061 aaaaactata aggaattaaa aaatagagtt aacagttgta ataagaaata ttcatgtatg
    60121 gactggtact tatgttttgt accagataat tataatgatg aagaagtgag caagaaaatt
    60181 gataatactt gtagctggaa aaaagaacat actaaaaaca ttgaaaatga atgtaactgt
    60241 gaacatgaac atcatgacta cctgaacaaa gctcttgact gtaaacaaga acacaagact
    60301 gatattaaag atgattgcaa tcatgagaaa aaacatacta aaaatactaa caaagttcac
    60361 aactccaaac aagataagtt taaagataag tcttgtgatg aaatgaattt taactatgat
    60421 aaagatgaat cttgtgacaa aataaattct agctataaca aagaagatag tagttatgaa
    60481 gatttctata aacataatta taaaaactac gattatacta gcgaaaaaaa tactaaaaaa
    60541 atagctatga aaactttaaa agattctaaa aaattagtta gaccacaaat aacagaccca
    60601 tacaatccaa tagttgaaaa tgcaaactgt ccagatataa atccaattgt agcagaatat
    60661 gttcttggaa atccaactaa tgtagatgct caactattag atgcagttat atttgctttt
    60721 gctgagatag accagtctgg aaatttgttt attccttatc ctagattttt aaatcaatta
    60781 cttgctctta aaggtgaaaa acctagctta aaagtaattg tagctattgg aggttgggga
    60841 gctgaaggat tctctgatgc agcattaaca cctacatcta gatataattt tgcaagacag
    60901 gtcaatcaga tgataaatga atatgcttta gatggaatag atatagactg ggaatatcct
    60961 ggaagtagtg catctggaat aacatcaaga cctcaagata gagaaaactt tacactctta
    61021 ctaactgcca taagagatgt tataggagat gataaatggc ttagtgtagc tggaacagga
    61081 gatagaggat atataaattc aagtgctgaa atagataaaa tagctcctat aatagattat
    61141 tttaatctta tgagttatga ttttacagca ggtgaaacag gcccaaatgg tagaaaacat
    61201 caagcaaatc tttttgattc agacttatct ttgccaggat atagtgttga tgcaatggta
    61261 agaaatcttg agaatgctgg aatgccttct gaaaaaatcc ttcttggtat accattttat
    61321 ggaagattag gtgctactat aacaagaact tatgatgagc ttagaaggga ttatataaat
    61381 aaaaatggat atgaatatag atttgataat actgctcaag ttccgtattt agttaaggat
    61441 ggagattttg caatgtcata tgatgatgct ttatcaatat tcttaaaaac tcaatatgtt
    61501 cttagaaatt gtctaggtgg tgtattctca tggacatcaa cttatgacca agcaaatata
    61561 ctggctagaa ccatgtctat tggtataaat gaccctgaag tattaaaaga agaacttgaa
    61621 ggtatttatg ggcaattcta atagaatgta gtaaaatttt atagttatat aaaaagcaga
    61681 ttttattata ttaaataagt tgtatctaat taatactatt attaattgtt tataaattag
    61741 atacttattt tttcataata caatctgctt ttttaatcaa aatttattta attacaagtt
    61801 gtttaattat aaagtaggaa tttagatata ctcttatacc atgtaattat gtataaatat
    61861 ttttttatta tacaccaata gcttttttag ctaatttatc agcttcctca ttatacttat
    61921 ttccagaatg agctttaacc tttgtaaatt ttacattaag cttattctta atactgtcat
    61981 agaaattttt atatgctata gttccttctt tatttgtttt ccaaactcca gtacaccact
    62041 tttcaatccc ttcatagtca aagtataaaa tcaagttttg aacattattg tcaatacagt
    62101 actgcattgc aattttagaa gcctctatct ctccagctac atttctcata cttactaaag
    62161 ttttctcttt tcctttttca ctataagttt ttataacttc attatttttt aaaatcacaa
    62221 cacccgaacc atacattttt actgaatgtt cataacttcc atctacatat gcttctataa
    62281 actccttatc ttcttgaaaa ctcaattttt ctgacccatc aatatattct ttagcttctt
    62341 gaaaagtaga aaaactctta tattcagctc ctgagaatcc atttacttgt tttttgcact
    62401 catcccatgt attatataca cctatatttt tacctttttt cacagcataa aacttttgtt
    62461 ttgccaaata tatcactccc actcttaatt tttatcttct tctctcataa atatatctaa
    62521 atttataaga tagtttctat caaaatattt tactttcata tcttttttta taatatcaat
    62581 atggtacact tctcctttta cagctctttg tcttatattt tcatttatat ctttttttat
    62641 aatatcctta tcaacatcat tgttatccat tttagcatca acattcaacg cttttatagt
    62701 atcaaatata acagatttta gtattaaact tctagattta tttaaatgtc gccttatata
    62761 agtataaaac tcattgctat taacctttga aattttacaa tcgtgtaaat ctttctctaa
    62821 tctctttaac acacttatat tagttcctac atcaaaaatc acaaaacatc catagtcata
    62881 tttgcttctt aaaattctcc ccatagcttg ctttaccttt atggtcattt gaggataatt
    62941 tatagtatag tagtctatac catacttctt cattatagta aaatacaaag ggtctttagg
    63001 atttagattc ggaagcttgt ctaaagttac acaaaccaaa ccatcccctg gaatgtctac
    63061 gccttcaaaa catccttttg agccaagtac tacacatttt ctatttaaat cgttaaggtg
    63121 tctaatgccc tttttatcag cataaatttc taagttaaaa ctatgtaagt attttttcaa
    63181 tatttcataa gttttttctt gtctatcttt actattaaaa agagctaatg tatgaccttc
    63241 tgtaatcttt gatatattag atataatttt actcatctca tttggaaatt cagaatttct
    63301 gtactcacaa atatcagaaa atccaactac agagactcta tttttataat caaaaattgg
    63361 ttctataatc ttttccacat tcttaactct gtctattcct aatgtatttt taaaataaga
    63421 catactttca gataaactca aagttgctga taaaaatatt cctttttcta attgagataa
    63481 aatattttct tcaaataaat ctgctatttt taatggtact actcgaaatt caaagtcatt
    63541 ataattctta tcaatttcaa ctatccttgc ataatcatcc ttttcatcat actctaaaaa
    63601 gatttcaaat ataatcttta tatcctctaa atctcttgtc tttgcctttc caaatttata
    63661 tatatctgct tctttatcta tactatcatc atcaatattc ctataaatta ttattattat
    63721 tgaaactaaa ttttttatta ttttctcaca actcaacttt atcttttcac tataggctct
    63781 ataacttatt tcagtatcta taccatcttt ttttagtttt cccacaattt catctatttg
    63841 caaattcagt tcccatctta aattataatt acttacatta ttatattcat tacaattacc
    63901 aaactttaat attgaatcta tctcttcaat aatgaaattt atacttctag caattttttg
    63961 tttatcttct cttccaattt ttaatacatt ataaaattta tcaaaagcct ttatttttct
    64021 catatttcta ctatattttt tatatataaa gctactattt tgaataaact cgtaaggata
    64081 aatttcttgt aataagtacc ttaaagactt tgaatttata atacttgaaa agaaatcata
    64141 tcctttttca gtcaaattat gtgcttcatc tactattata ttttctaaag gtttttcatc
    64201 cttatacggc catttagcta atagtgaatg atttactact gttatatgtt cttcttttaa
    64261 ttcttctatt ctgtttttat ataaacaatc ttttttgcat tttttaggtc tgcacatatt
    64321 tgggtcacaa cttacatttc ttaagtgagt acttatttcc ttgaagttgt caaacactaa
    64381 ataatttatt tcttctatat ctccatactt gccaccttca actaatctct tcaaaaatat
    64441 taaagataaa atttctcctt tagtagggtt ttgagactca taatcatcta tatatgcttc
    64501 cagtctatct atacaaatat agttattttt tccctttata tatccgtaac ttacttttcc
    64561 atttagaccc aaagaatcta acacatttgg aatatcttta tttattaatt gtatctgcaa
    64621 ttcctttgtg tcagtagaaa ttaataatct ttttttatta attctagcct ctaatattgc
    64681 aggcaataaa tatcctacac ttttacctat accagttggt gcctctatac atgcaatttt
    64741 ttcttcatct tcgctatttc taaacaactc tcttattgtt ttagtaagct catattgacc
    64801 aggtctatat tcatatgtaa atccttcttt attctcccaa atagttttgt attttaataa
    64861 ttcttcataa tttttttctt cttcatttaa aatttttaag gtttctcttt ccttcttctt
    64921 ttcttctttt gagtcaaata tattatattc tttttttata tttatattat ttgataaatc
    64981 ataatttgcc tcttctaaaa atttgctcca ttcccatgtt ggaagattaa attttttaag
    65041 atatgaattt attttaaaac tcaatggctc caaagtagtt ttttcatagt tattaaattt
    65101 tactaaaagg caatttacaa tatttattat atcaatagca tctgataaag ctctattttc
    65161 cactttagaa ttacaatttg ttaaagtatt ttttaagtat tctaagctat aatctttatg
    65221 atatggttct aaaataactg ctaattcaat taaatcaata aatttatttt ttaactttgg
    65281 catatgatac tcaaaaaact tttttttcag acttaaatca tgacatataa tgaaactatc
    65341 ttctacaaaa tttacaagtc tatcttccac cagttctatt ttattagcta tttctaaatc
    65401 atttttatct aaatttttac atactgagaa tacttctata ggtacctcaa atttgttttt
    65461 tattaatgat tcataggtat atattttcca gtctttaacc ttgacagctc caacttctaa
    65521 aatttctgaa tttaaacaat ctaatccact aacttctata tctaaaaaaa caacatcatt
    65581 caatacactt attatattat ccatttttat atcccttcaa gttaaaccag cctaacttat
    65641 ttacaatcat ttctctttat attttatcat ttatatacac tttatttaag ataattaata
    65701 tcaactttat atcttacagt aaacttcttt catttattat ttgtatatgt gaatattttt
    65761 aactataaat attcaaacta tattataaga aatatacttt aaattttaaa taagaggtga
    65821 aattgtgaaa aaagatagcc taaaaaaata cattatgata ggagcatttg ctctaacact
    65881 ttttggaatt gctagtataa attttaaatc attagataaa acaaaaactc aaatttctag
    65941 tccaacactc gacacacatg aatatgattg gtattttaac cctagggaag atggtaaaca
    66001 gccatcacct ataaaagaag ctgatttttt taaaaaatac ggagcttatt atgtaggaaa
    66061 tcccaatgaa aaagttatat atctatcatt tgatgctgga tatgaaagtg gaaacactcc
    66121 aaaattatta gatacgctta aaaaacacaa tgctaaagct caatttttcg tagttgaaag
    66181 ttatataaaa agcaatccag agttaataaa gcgtatggaa aaagaaggac atttagtttg
    66241 taatcattca aaaagccatc catcaatggc aggtattaca gattttgaaa aattcaaaga
    66301 agaaattaca agtgtagaaa aagcatacaa agatgttact ggaaaagaaa tgcctaagta
    66361 ttttagacct ccaatgggta aatttagcga acaatcttta aaatatacac aagatttagg
    66421 atataaatca atattttgga gttttgctta tgtagattgg tatgaaaaga aacaacctac
    66481 tcatgaattt gccaaaaaca aaatttattc aagaactcat cctggtgcaa tagttttact
    66541 gcatccaaat tcatcaacaa atactgagat attagatgag gttcttacac attgggaaaa
    66601 agaaggttat aaacttaaaa cacttgatta tttaaataat aaaaaatagt atcataaatt
    66661 taaaaaaact ctatcatgaa ttatagtaaa aatatagtta tttataaata ctttaattct
    66721 cgatagagtt ttttattttt tatagctatt taaaaagaat tgtccattca tttatctctt
    66781 tgtatcctat attatgatat attttacctg ctacatcatt attataaaaa agacatggaa
    66841 cttttccttc taacaacaat tctttactca aattgtaaac catataactt gcaagtcctt
    66901 ttcccctata tcttttatct gtacttacag agaccaccat tgccaaaaaa ctagtttcca
    66961 taccagttga cacagtgcta atcatagtat catcatttct aataaaatat gctcttacat
    67021 ttccctcttt taaatgaact tttctttctt ctatgtaatt tttacttact ttatagtctg
    67081 acctatttaa aagttttcct atttcatcaa tatctttttc ctgagcattt tctattttat
    67141 actccttcat gttggaaaaa tctatttctt ttatttcttt taatacacaa aatttattat
    67201 ctagtttttc ataaaaatct ttgtatttac ttattagtct atctattaca catttttttc
    67261 cacttatgct ttctacatct aaatctttta tatgattctt catttctcct atattaaaat
    67321 tatttttaaa actataaatt ataagtgttg acttattttt taatattata gaggttatat
    67381 ttccaccttc tacctgagac catatatctt gatattgaga gctaaatcca taattctcaa
    67441 cgtatccaat tatgaataaa tttaaacttt cttcatgtga tacatattgc caaaattctt
    67501 tttctaaagt attatctatt tttacaatca agcagatatc ctccttctat gttaataatg
    67561 atatacctta taatttatag tatcatttaa aatcattata ttctaccaaa tattctaaat
    67621 ttttaaatat ctatgcaata tgttcgtatt tatttttata ttctgttact gcttcactcc
    67681 aagcttcttc tactgaaata cctctcttat ttgcaatttt ttgtgctaat tctattattt
    67741 tcatagcttt tcttgatata ttcatatata ttcctcctct aaattatcat aagttatttt
    67801 aaagttaaat ttctatagtc atgttatata tttatttact tatatcttac taattattta
    67861 tgtactaaat atgaactaca atagaagaaa tttagaactt aataaaacat aaaatttata
    67921 tacagttcat atatatttgt catatcacaa gacaagtgat ataatttatt atcattttac
    67981 tgggggtgat atgctatgaa aataagagct gaatatacat gtcctcttga aattgtacat
    68041 gacataatca aaggaaaatg gaaaactatc cttatatttg aattaagaca atctaataaa
    68101 acattttctg aattgcaaca taatatttat ggaataaatc aaaaaatgtt gattcaacaa
    68161 ttaaaagagt tacgtgattt tgggattatt gataaaataa gttctgctgg atatccatta
    68221 catgttgaat attttcttac taatcgtggt aaaaaaatgt taaaagcagt ggaaataatg
    68281 caagaaatag gaatcgaata tatgcttgaa catggtcaac aagaaattct agataataaa
    68341 ggaatttgct acaataagta atcctataca taccaaaaag taagtaattt acatttttta
    68401 caatagtatt ataataaaaa taaataggag gtataatcat gtctatagct ttaatattga
    68461 ttgatattca aaatgattat tttaaaaatg gaaaatgtga attatttcaa tcagaagaaa
    68521 ctgctgaaaa tgctaaaaaa atactactgt tttttagaaa aaacaattta ccagttattc
    68581 atatacagca tatatcaaca gataaaacag cttctttttt tcttcctaat actcatggtt
    68641 cagaaataaa taaaattgtt tttccactag ataatgaaga aattataatt aaacacacac
    68701 cagatagttt ctttgaaaca gatttgcaaa gtatccttga gaaaaaaaat attactaatc
    68761 tagtaatctg tggaatgatg agccatatgt gtattgatac aagtgtaaga acagcaaaga
    68821 aacttcgata cgacataact ctaataagcg atgcatgtac aacgaagaat ttatcttgga
    68881 ataatataga aattaatgca aatactgtac atcaagtatt catggcatca ctacagaaca
    68941 gttttgcaga tgtaaaaaac acagataaat ttttatctaa ctattacaaa taaaaacata
    69001 cactatgtta aaagagcaag tttgatttat ttaagcttgc tctttaatca tttaaaatta
    69061 gatgctttct atcattttta tatacataat atacgtttat acattttata ttcatatata
    69121 actattatat aaattttaaa ctttactata ttcacataaa aaatgtagta ttatagtata
    69181 tataatatta tatagaaggg ggtgctagat aaatgaacca tcatatattg attcaatttg
    69241 aatatatagc aagaatcatt actgctggtt tctgtggggc tttaattgga tatgagagaa
    69301 agaatagact aaaagaagct ggaatacgca ctcattttat agtggcatta gcttcagcac
    69361 ttataatcat tgtttccaag tatggattta atgatatagt gggtactcca ggtattggtc
    69421 tagacccagc ccgcgttgca gcacaagttg tcagtggtat aggtttttta ggtgctggat
    69481 taatatttat tagaaatcaa tcagtaagtg gtcttactac agctgctggt atatgggcaa
    69541 cagccggtac aggtcttgcc attggctctg gattatactt agttggtatt gtttcaagta
    69601 tcattattgt tattgtacag ataacacttc atagagatag gaaatggatg aaactcccta
    69661 cagcagagca agtttctctt aaaatttcta acagcactga ttccatagaa tatattgaag
    69721 gtaaatttaa aaaatctaat attgaaatta taaacatgaa tattaaacgc cttgatgggg
    69781 attggttaga agtagttata tatactaagt tacctaaagg atataaaaaa acaaatttat
    69841 taaaattatt tagcgataat cctcttatag aaactttaga aatataatat aaaaattgaa
    69901 gcttaaaaat aataatatat ttaatcaagg tcaatatata aataattaag ggatgagtct
    69961 atataaaatt catcccttgt tttttgccat tcatatatat tttttcaaga tatttttgtt
    70021 tatctattat ctagccaagt aacccttgtg aactaaactt tttaatacaa aaacttcaat
    70081 acaaaactaa atataatttt tattaaacat tctatacata tagtttgttt cttgagtgaa
    70141 aaatagattt catattgtta agatattgtt aaatatttca attttctgac aagtatgttg
    70201 aacaataact tatcctagtg tattatttta gttgtagaat aaaactaaat cggcaaaact
    70261 agagaaattt agtgacgcaa agctataggg actaacgctt ataaaataaa ctatgagtta
    70321 tgtcagccag ttgccaaaaa gatattgttc tttttgtttt atgaaagttt tttaggggga
    70381 tagtaatata tatgtttaga gatgaaatgg ataaatgtac acacatgtta actgcttata
    70441 ttagtagttt atatgattat tgtgatttta tagatacaca gctagatgat tttatactag
    70501 agtacggaga aaatgtagta gaatcttgtt tacatcaagt gatggtattg gtaagtaagt
    70561 ataattaaat agaaattgtt ttctagagaa ccctttttag ttttatctac atttaaaaat
    70621 gttattatat taaaaaacta atcttccata tatgcaaagt tttcttccat ataacaattt
    70681 cttcccattt atactttttc acttgtatct ttaataaaat tcaacttcaa aaattattct
    70741 atttcttatc tttacaccaa ttcctatcag tattatcctt tatataggtt ctaactattt
    70801 cgccacaatc taaacataaa tctgaatata tagtagattg tactggaaaa gttttattta
    70861 taactttatc taattttcca tctcctctat attttaatcc aatagtttca gcatatatgc
    70921 caccagttgt taaaattccc ttttcaatat ttacacttcc acaattagaa catttcacat
    70981 ttataacccc ctaaacaatt attttttcat gctatcttat aatttaaaat ataatttgct
    71041 accgatttta taacacagtg gcaaatcggt gccaaatcaa cattttttca ttctatttac
    71101 catctaatcc attgaaatat agatatttag ttataggaac ttgaatttct gttacaaact
    71161 cctctacaat tccagtctca tgtatatcta tcttatagat ttctataggg tcagcaatta
    71221 tcttataatt attttcttct ataaatttaa acaccttctc catatacatc ttattattag
    71281 aattactccc agtataattt aaagttacat aatatccttc atcaaaaact atattatata
    71341 ctttctcatt actttccaga aaacaaaata cagatttaaa ctcattaaat atgcctttat
    71401 taattgcttc agtagaaaag gcagaaccaa tattgttgtt tccaagtata ttaaatctat
    71461 cttcatattc tttctgaagt ttttgaatta aaaaatcgaa gtcttcatcc ctctttatat
    71521 cagcatttaa ttctaaagct tttctttttt ctatatatac aacctctatc tcatctattt
    71581 ttgtattttg aattacactt tcaattgaac tcaatctttt tataatattt tccttatggc
    71641 ttataaattc agctattttt ttatcaatta aatcgagttc ttcatttaaa atatttttcg
    71701 tagactctat acttctgtca tctaggtatt cttttatttt cttcattgga aagttcaagc
    71761 ttctaaattc ttttattaaa tttaatttcc atatatctga tatattatac attctatatc
    71821 catttatatc tctaactggt cttagaatgc ctatttcttc ataatacatt aatgaatctc
    71881 tacctattcc atatatttta gatatctctc ctattttata gtaatctttc atttatataa
    71941 taaactcctc cgaaatctta tttttatttt aaatcaacat tattactcta taatactata
    72001 gtattagaat atactaaaaa aatctttaaa acaaggatag gaaaataaac atcctaataa
    72061 ttaattatta aaatattttt atattgtaat attaggtata gtatttataa cttcaacttt
    72121 tttataaata tattttagtt ttatatagtt ttat
//
LOCUS       DAYATN010000006        65747 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.006, whole genome shotgun sequence.
ACCESSION   DAYATN010000006 DAYATN010000000
VERSION     DAYATN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 65747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.006"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            40..195
                     /locus_tag="ACGI8A_000381"
     CDS             40..195
                     /locus_tag="ACGI8A_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178393.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            325..1338
                     /gene="aroF"
                     /locus_tag="ACGI8A_000382"
     CDS             325..1338
                     /gene="aroF"
                     /locus_tag="ACGI8A_000382"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFH0178394.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1360..2424
                     /gene="aroB"
                     /locus_tag="ACGI8A_000383"
     CDS             1360..2424
                     /gene="aroB"
                     /locus_tag="ACGI8A_000383"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFH0178395.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2424..3737
                     /gene="aroA"
                     /locus_tag="ACGI8A_000384"
     CDS             2424..3737
                     /gene="aroA"
                     /locus_tag="ACGI8A_000384"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFH0178396.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3715..4794
                     /gene="aroC"
                     /locus_tag="ACGI8A_000385"
     CDS             3715..4794
                     /gene="aroC"
                     /locus_tag="ACGI8A_000385"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFH0178397.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4797..5993
                     /gene="pheA"
                     /locus_tag="ACGI8A_000386"
     CDS             4797..5993
                     /gene="pheA"
                     /locus_tag="ACGI8A_000386"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFH0178398.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSKFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEMISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6040..6849
                     /gene="aroE"
                     /locus_tag="ACGI8A_000387"
     CDS             6040..6849
                     /gene="aroE"
                     /locus_tag="ACGI8A_000387"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFH0178399.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6861..7394
                     /locus_tag="ACGI8A_000388"
     CDS             6861..7394
                     /locus_tag="ACGI8A_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFH0178400.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7415..8254
                     /locus_tag="ACGI8A_000389"
     CDS             7415..8254
                     /locus_tag="ACGI8A_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFH0178401.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            8522..10003
                     /locus_tag="ACGI8A_000390"
     CDS             8522..10003
                     /locus_tag="ACGI8A_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFH0178402.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            10036..13470
                     /locus_tag="ACGI8A_000391"
     CDS             10036..13470
                     /locus_tag="ACGI8A_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HFH0178403.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDGIEKGIWILNDI
                     TDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNVP
                     YSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDED
                     GNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESWQ
                     KPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRRM
                     NQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYNK
                     GTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPIF
                     RKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGIS
                     FVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADKC
                     EKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDRP
                     CNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            13488..14234
                     /locus_tag="ACGI8A_000392"
     CDS             13488..14234
                     /locus_tag="ACGI8A_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFH0178404.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(14183..14491)
                     /locus_tag="ACGI8A_000393"
     CDS             complement(14183..14491)
                     /locus_tag="ACGI8A_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFH0178405.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            15295..19437
                     /locus_tag="ACGI8A_000394"
     CDS             15295..19437
                     /locus_tag="ACGI8A_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HFH0178406.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NISTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNI
                     AEITSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
     gene            19561..19764
                     /locus_tag="ACGI8A_000395"
     CDS             19561..19764
                     /locus_tag="ACGI8A_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178407.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMQEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
     gene            19784..20689
                     /locus_tag="ACGI8A_000396"
     CDS             19784..20689
                     /locus_tag="ACGI8A_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFH0178408.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEIVDLGLGLPVKQFAGLRKNLIEK
                     LQGKEYHVEFTRGNETTKRDITIRSIQTFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSINVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYINK
                     GFYLNGEKQDIKKNIDASISLFKEIYNELKLNYPVSTAAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            20772..21287
                     /locus_tag="ACGI8A_000397"
     CDS             20772..21287
                     /locus_tag="ACGI8A_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178409.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRTGNKFVEDNKSYDDKYHIDSISSKIDDDIKYLSSEIDNIKNVLEWFKTK
                     DDSYHICVIKENDISIDLPDQSIKRTTVRINNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(21298..21564)
                     /locus_tag="ACGI8A_000398"
     CDS             complement(21298..21564)
                     /locus_tag="ACGI8A_000398"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0178410.1"
                     /translation="MSKKEIIRICNEKHNWRKSATKTLLKRLVKKRVLLKRIKIFHIY
                     YIPIVTEKEYFKYKINDLEPDYLDEYFIRLMSTVHKFKNLDEED"
     gene            complement(21771..22877)
                     /locus_tag="ACGI8A_000399"
     CDS             complement(21771..22877)
                     /locus_tag="ACGI8A_000399"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFH0178411.1"
                     /translation="MLTFLDLFAGIGGFRLAMERAGHKCVGHCEYDKFANLSYKAMHN
                     PKEDEWFEKDIREIRAENIPKADVWCFGFPCQDISIAGNKLGFKGNRSSLFFSVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNRGFDFAKLLIELDEIGYNAEWQNLNSKNFRVPQN
                     RDRIYIVGHLRRRSTREVFPITGTSRKTTLEQLNNPNHSSNRIYNSNGLSKCLLSKGG
                     NNTGLYRVGNINPSGNGAGGQVYSSEGFSPTLTTGKGESPKILIKENTKKGYIKASIG
                     DGIRLDHIEGKTGRGRVQHKKSCTLTTASNAGVIGKKFRVRRLTPKECFRLQGFPDDY
                     YDRAASVCSETQLYKQAGNSVTVDVVEEIAKKLK"
     gene            complement(22949..23779)
                     /locus_tag="ACGI8A_000400"
     CDS             complement(22949..23779)
                     /locus_tag="ACGI8A_000400"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0178412.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADIFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPRVNAGGYDLLIELHLNASDRQGKGSEVLYYSN
                     KGLEYATRICKKLGTVFKNRGAKLDKSLYILNSSKPTAILIESFFCDNKEDYEKAKKL
                     GYEGMAKLIVEGVLNKSIESNEVKQMYKHTIVYSGDDRVSADILGLYYKRAKESYLVI
                     DIKDYKPHRTQNLYVIGGVTCNKMKEMSKTTGEKFTQLYGNDVWSTMDKVIEFVKEKL
                     "
     gene            complement(23783..24211)
                     /locus_tag="ACGI8A_000401"
     CDS             complement(23783..24211)
                     /locus_tag="ACGI8A_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFH0178413.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGK"
     gene            complement(24270..24839)
                     /locus_tag="ACGI8A_000402"
     CDS             complement(24270..24839)
                     /locus_tag="ACGI8A_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178414.1"
                     /translation="MTFKELVSKVRNLVLEAKNVTIEDTENKFTSENVEGALKECIDR
                     ADLAFQSADSGKELIATAIGSPATSEQTFQEYANYIAGFKNTISQLNSKYKYTSGTFT
                     YYTGAYPNGLNIGFRPDIISYTAINAVEEKYYCVYTPLFYLFYNYTTGEKYLDVNHNR
                     YGITFTDNGWIPRNPVNNGVYSYIAAKCR"
     gene            complement(24869..25039)
                     /locus_tag="ACGI8A_000403"
     CDS             complement(24869..25039)
                     /locus_tag="ACGI8A_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178415.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKMDVFYAVNRITEEQYLELTGL
                     LNKEETPVEPTI"
     gene            complement(25051..25398)
                     /locus_tag="ACGI8A_000404"
     CDS             complement(25051..25398)
                     /locus_tag="ACGI8A_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178416.1"
                     /translation="MNRNNRIIYDQTGKIWLQTGEAIGDIQELSEITELNFIDVEFGS
                     IDYSKQYIESINPVTKELNIKNIEVVLTDEQKRLEVLEKELNMLKEENKNRDSEIVNT
                     AFEVGNIKLNNNL"
     gene            complement(25422..26867)
                     /locus_tag="ACGI8A_000405"
     CDS             complement(25422..26867)
                     /locus_tag="ACGI8A_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178417.1"
                     /translation="MKIWKYNRTVKKRRGVYTELDILAQRYEFEADGKLYTAYFHLYT
                     NKNIDIFRDKDLYKESYMMSYRVGNVTTDIDDVVQQTTTVRFIPDPVIDIVIDNISRG
                     CEINYRIIDNEPSVKFIVTEKLNGTIIETKTNSSDNNYKLKLTDEQIVPLAFNSQNSI
                     VIEISTEDGGLVTSKTVTFTRTNNKPTILVNSYSSNSAKFTASDLDNNLSKIEWYLDD
                     VLKETITTDLTAEKTINYEFTDNAIHTLKIVATDAENATVEKVLSISKEIMPLQSDAS
                     LSDISTKLIEIGEGFRNGKTSIINTLALKNIEASLNNTLVELSEKIKTSFDSSDASVQ
                     DLQNRITELNNQLSRRKKWATGRYTFTREEDTINFRMVDGDSLSAVKKLIIPINLDFV
                     PSVIIIDKMFLGSYVPAQDVQFNCVSNLADFTLGRRFYNKQWICTSSIKLDSVTQNSF
                     TLSLQKVVPFDKVSSTIFSIINTGFAWRAYE"
     gene            complement(26884..27666)
                     /locus_tag="ACGI8A_000406"
     CDS             complement(26884..27666)
                     /locus_tag="ACGI8A_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFH0178418.1"
                     /translation="MTEQQYFTLVTDIGKAAIANASITGEKVDFAKIKVGDGGGSSYT
                     PNESQTALKNVVWESTLEHAQGDKDNPNWVVIQKFIPGDVGGFEIREVGLFDSKDQLL
                     AISSYPTTYKPTADSGTVKELLIKVILVVSNVANINLKVDPTVILATLKDIQELDTKI
                     DTTKTELTSNIETAKTELNTKIGDTTQLTTTDKTNIVSALNEVKTSVDSIETTADKTS
                     IKDTDNLFESDNVEGALKEVMQEVKGNRSSIISTINSNLIPM"
     gene            complement(27683..28291)
                     /locus_tag="ACGI8A_000407"
     CDS             complement(27683..28291)
                     /locus_tag="ACGI8A_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFH0178419.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLEQFFID
                     TATWGLDDWENLLSIKVNYKLDFDTRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPAHLEHKFERILFNKNELYTGAA
                     ISTGETVTIYPYVPRNLESFGEIAICSGNDRALEKVTLYPKK"
     gene            complement(28293..29345)
                     /locus_tag="ACGI8A_000408"
     CDS             complement(28293..29345)
                     /locus_tag="ACGI8A_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFH0178420.1"
                     /translation="MYNGQSFSTLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVTFEGKIGTQIPNGTII
                     SHSDLLFVIIKDITIDENSKLNVSPIQALEIGIKYNLSANTEFKLIEEITGVTKIYNE
                     LDLKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCTEHIEEEKPIGPTVTIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIVYIKVMSLLASIEGIHDIKNLLVNGDVKNIIVDE
                     EKVPAVSSAIFDIEVS"
     gene            complement(29338..29772)
                     /locus_tag="ACGI8A_000409"
     CDS             complement(29338..29772)
                     /locus_tag="ACGI8A_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFH0178421.1"
                     /translation="MNNTIFPFMGVPEDYISPDNEELPIFKEFAWDFNKDEKIIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVHLIYDWSYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNITNAEFEDDNLSISLNVKTIYGEEEITFV"
     gene            complement(29765..30097)
                     /locus_tag="ACGI8A_000410"
     CDS             complement(29765..30097)
                     /locus_tag="ACGI8A_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HFH0178422.1"
                     /translation="MADPINEFIGIIRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGNTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            complement(30111..31628)
                     /locus_tag="ACGI8A_000411"
     CDS             complement(30111..31628)
                     /locus_tag="ACGI8A_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFH0178423.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFVDNKFPLGNLPKTNVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESIENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYPAEFTAYCPR
                     KEEGGNTDCRGKKLDPSKKTCAAPMVGAYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNMTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            complement(31869..32504)
                     /locus_tag="ACGI8A_000412"
     CDS             complement(31869..32504)
                     /locus_tag="ACGI8A_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFH0178424.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDIYFTLSLKEYRRIQISKVNINNDEKLSSEKNVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYSNGDLWEKIYKANEDEIKDPSVIKNGWVLIIP"
     gene            complement(32562..33038)
                     /locus_tag="ACGI8A_000413"
     CDS             complement(32562..33038)
                     /locus_tag="ACGI8A_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFH0178425.1"
                     /translation="MNKKILVLTISIILSLSLVGCSTNNSKNRDSKSKQNTEEVKNHK
                     IGEVVDLDGAKVSVKNIRKLETNSNDYAVEIEITNTGDNDLQSDYLENYILKSSDNTT
                     GEAINNQELNGELMFDTIRPGDTLKGEIAFELTKDATPKTLEIAYENVGKYTVFDL"
     gene            complement(33085..36474)
                     /locus_tag="ACGI8A_000414"
     CDS             complement(33085..36474)
                     /locus_tag="ACGI8A_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFH0178426.1"
                     /translation="MSSNTKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRALN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKGMTDEQKNYYLSAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGIFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPVGWTITAVIALSVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFDLIIGIVTGDSKKMMDG
                     VKQIFDGGTKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWRDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTVANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKDCFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSLFKDKVKGVKEAWNKLKDFFKHPIKGTIEI
                     FKKGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKIGGVL"
     gene            complement(36478..36627)
                     /locus_tag="ACGI8A_000415"
     CDS             complement(36478..36627)
                     /locus_tag="ACGI8A_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178427.1"
                     /translation="MYLLFKYKGIMPSVSYHILRGEKEIVSVFMDQELDERKEEIKSG
                     VGRLF"
     gene            complement(36654..37070)
                     /locus_tag="ACGI8A_000416"
     CDS             complement(36654..37070)
                     /locus_tag="ACGI8A_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0178428.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSAFELYCKKLDDTLEIECKAIDPER
                     FDEIRMNSMDVNSGDVENINIYELKVNTILESCSIFSNMELVKKFSAITPKELIKKLL
                     LSGEIDKLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            complement(37085..37525)
                     /locus_tag="ACGI8A_000417"
     CDS             complement(37085..37525)
                     /locus_tag="ACGI8A_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFH0178429.1"
                     /translation="MKAKIDAKRIISGTFGECWLDNKQLTSTKGMEAKVDIDKEEIKL
                     CGQIMTTYKMIGATGKGSITLYKISTLFGEYVEKMLNEGKEFKVTIISKLQDPDSYGS
                     ERIALYGCMFDDLTLADWEAAKVGEIEVPFTFEGFEYLDKIKEK"
     gene            complement(37541..38596)
                     /locus_tag="ACGI8A_000418"
     CDS             complement(37541..38596)
                     /locus_tag="ACGI8A_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFH0178430.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEIE
                     DIPENYSAANLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     ETEQDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDEGSTVEINLDAQRAWLKKQNIDISNMEEQQIKEYNTDTLVFLKA
                     RIKLIDAMEDVYLDISM"
     gene            complement(38597..39046)
                     /locus_tag="ACGI8A_000419"
     CDS             complement(38597..39046)
                     /locus_tag="ACGI8A_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFH0178431.1"
                     /translation="MLTNRDIIIAVNKRIENEFRYIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKVLDTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLIIE
                     NISPSIAKDPVGYKLTFLMTVSYFEEVYFENETFEIMESVDLEIGSD"
     gene            complement(39055..39411)
                     /locus_tag="ACGI8A_000420"
     CDS             complement(39055..39411)
                     /locus_tag="ACGI8A_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFH0178432.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVKTEKLKKGWEEKQPEYDTYIVKNEVDYATLVNNGHSTKNGGIVPGSFMVEKGIE
                     RTKENMTSAKANLFDW"
     gene            complement(39411..39758)
                     /locus_tag="ACGI8A_000421"
     CDS             complement(39411..39758)
                     /locus_tag="ACGI8A_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178433.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITELERI"
     gene            complement(39770..40141)
                     /locus_tag="ACGI8A_000422"
     CDS             complement(39770..40141)
                     /locus_tag="ACGI8A_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFH0178434.1"
                     /translation="MLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELSTAL
                     EGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENSISS
                     SSFLTDTDKKFLSPFCIVSMY"
     gene            complement(40134..40406)
                     /locus_tag="ACGI8A_000423"
     CDS             complement(40134..40406)
                     /locus_tag="ACGI8A_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFH0178435.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            complement(40420..41343)
                     /locus_tag="ACGI8A_000424"
     CDS             complement(40420..41343)
                     /locus_tag="ACGI8A_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178436.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            complement(41372..41974)
                     /locus_tag="ACGI8A_000425"
     CDS             complement(41372..41974)
                     /locus_tag="ACGI8A_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFH0178437.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            complement(42000..42326)
                     /locus_tag="ACGI8A_000426"
     CDS             complement(42000..42326)
                     /locus_tag="ACGI8A_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178438.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            complement(42385..43146)
                     /locus_tag="ACGI8A_000427"
     CDS             complement(42385..43146)
                     /locus_tag="ACGI8A_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFH0178439.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            complement(43124..44401)
                     /locus_tag="ACGI8A_000428"
     CDS             complement(43124..44401)
                     /locus_tag="ACGI8A_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_process: GO:0099001 - symbiont genome ejection through
                     host cell envelope, long flexible tail mechanism [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0178440.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            complement(44414..44602)
                     /locus_tag="ACGI8A_000429"
     CDS             complement(44414..44602)
                     /locus_tag="ACGI8A_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178441.1"
                     /translation="MANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRARSKSY
                     ISKQDIPIKVLKFIESTG"
     gene            complement(44618..46357)
                     /locus_tag="ACGI8A_000430"
     CDS             complement(44618..46357)
                     /locus_tag="ACGI8A_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178442.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLSKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            complement(46912..47364)
                     /locus_tag="ACGI8A_000431"
     CDS             complement(46912..47364)
                     /locus_tag="ACGI8A_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0178443.1"
                     /translation="MNEVEIIDSSYEKGKELTEDQCNMVTLLITGATVTETARIIGVN
                     RKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVMLA
                     ANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            complement(47585..48229)
                     /locus_tag="ACGI8A_000432"
     CDS             complement(47585..48229)
                     /locus_tag="ACGI8A_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0178444.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RVGDILALKVADVTDLKLDKKGNKIRVSKDWIRVIEEKRDYNREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAKKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            complement(48233..48778)
                     /locus_tag="ACGI8A_000433"
     CDS             complement(48233..48778)
                     /locus_tag="ACGI8A_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178445.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDITEEISSAAYVPDNLKYYDNVM
                     KETPGMYGRECSLDFIKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILEKSIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFTDVVE
                     DLKILVQERQRKNYYGNKECF"
     gene            complement(48921..49364)
                     /locus_tag="ACGI8A_000434"
     CDS             complement(48921..49364)
                     /locus_tag="ACGI8A_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFH0178446.1"
                     /translation="MQSSKKDNLFSSAEGKLYDYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTGKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            complement(49473..49730)
                     /locus_tag="ACGI8A_000435"
     CDS             complement(49473..49730)
                     /locus_tag="ACGI8A_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178447.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPRE"
     gene            complement(49750..50235)
                     /locus_tag="ACGI8A_000436"
     CDS             complement(49750..50235)
                     /locus_tag="ACGI8A_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178448.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKNAIDTGITKDIIAEG
                     LDVCQTILTMFQVLEIENYITEGVETHNKKLNGRGWDLKEIGDDILNLIKNSMINELI
                     NMNKKLKCLDEKEFNAKIQFLDVDKLWNLCMPLVNLSTIKGVNESIDEMKMGEFLKNI
                     N"
     gene            complement(50259..50519)
                     /locus_tag="ACGI8A_000437"
     CDS             complement(50259..50519)
                     /locus_tag="ACGI8A_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178449.1"
                     /translation="MKCKVYHAIKNIKDANELIDRFETENIFFELNDIDNENVLNGEM
                     NLTDIEDIASLQFILDGDLFIFSSCDEMIFMNKYTQKELGLQ"
     gene            complement(50544..51350)
                     /locus_tag="ACGI8A_000438"
     CDS             complement(50544..51350)
                     /locus_tag="ACGI8A_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178450.1"
                     /translation="MKFKTFEITKREFLVSIIIACMMIVLGFFIDDKVQSKFLEDNER
                     YYKATKINNDKDLFDYAMETSAGDSLISGRFKSVDSVSIEELKGDYWFIERTEEKYTM
                     HTRVVTTKDSKGHSHKYTETYWTWDYHDSIEKKCNKFNFMGKEFDAKVITNLSKERLA
                     LKKDIFKNNNLKIKGNYAYINNKRRYYYEVIPKEFNGTMYANLKNNTIANPADKNIKV
                     FKGESIQNVLENNNFNTGFFKVVFWIIWILITTIIIYVFYYLKNDWLEDK"
     gene            complement(51362..51898)
                     /locus_tag="ACGI8A_000439"
     CDS             complement(51362..51898)
                     /locus_tag="ACGI8A_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFH0178451.1"
                     /translation="MKKTLIIVGVVISILIMFACLFLVTKNKAIDLEEQVQSATASVE
                     VQEKRRIDLIKNIVDCVKDYAKYENRTLKEVTDMRVSANDESIKEAKLNIQAVAEKYP
                     DLKANENYKQLMSELSITENMIAQHRENYNMQVRNYNKYIRKFPANLILNSMGYEKIE
                     SKYTEYKAKEDAPQNLFD"
     gene            complement(51898..52224)
                     /locus_tag="ACGI8A_000440"
     CDS             complement(51898..52224)
                     /locus_tag="ACGI8A_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178452.1"
                     /translation="MACVIKCDFCGETIAEILNIKVFRIDDEGNIMSIGKDVCDKCYD
                     RLFRSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQECLGGCLEGRKKWL
                     ESEAMK"
     gene            complement(52227..52385)
                     /locus_tag="ACGI8A_000441"
     CDS             complement(52227..52385)
                     /locus_tag="ACGI8A_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178453.1"
                     /translation="MKTKIIGGRENQCPLCNGSVFKIETSIGIVWQCKDCGCMYQDMN
                     CKENKRRH"
     gene            complement(52415..52963)
                     /locus_tag="ACGI8A_000442"
     CDS             complement(52415..52963)
                     /locus_tag="ACGI8A_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0178454.1"
                     /translation="MRSLEERNKLVEENINFVWFIIRRYFKVFISKYPYLQDDTFSEG
                     CIGLCKAADYFDSKKGIEFTTFAAVCIKGQIFKFISRYVKKHYRADIDSFEKCIYKNT
                     HGEEIRIEDRLAGNENVDIENVHVIRSCIKRSKIEDIQKITYLREKGYTQKEIGKILR
                     TNQMTICRKLGKLKSEINILGK"
     gene            complement(52980..53357)
                     /locus_tag="ACGI8A_000443"
     CDS             complement(52980..53357)
                     /locus_tag="ACGI8A_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFH0178455.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYTKKDGTR
                     EVDFIPIEVMGGSAEFCANYLAKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DRKPKDEEKEISFQVLDDEDIPF"
     gene            complement(53354..53536)
                     /locus_tag="ACGI8A_000444"
     CDS             complement(53354..53536)
                     /locus_tag="ACGI8A_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178456.1"
                     /translation="MKMISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            complement(53593..54297)
                     /locus_tag="ACGI8A_000445"
     CDS             complement(53593..54297)
                     /locus_tag="ACGI8A_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178457.1"
                     /translation="MIRNAMEYLVKLGKRETVCVDGFKYTTDALEIIKEPKANELEIT
                     TLSGLVDYIKSGIDHKADELLLIQVLTPRKVLLKSPLRKNRDRETYIECVALLPEKRF
                     DRPLDLDSFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEIEQPESEFIFRMSSGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            complement(54350..54736)
                     /locus_tag="ACGI8A_000446"
     CDS             complement(54350..54736)
                     /locus_tag="ACGI8A_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178458.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSAGVIDFRNASSK"
     gene            complement(55111..56442)
                     /locus_tag="ACGI8A_000447"
     CDS             complement(55111..56442)
                     /locus_tag="ACGI8A_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFH0178459.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDIGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIIDINDLKALVTTSSSVNDNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAQAITNGEKAFLYSG
                     ELPSFMSMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYAVELISDWIEDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRFFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVVDEEEETDDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQVEIPEDVF"
     gene            complement(56464..56781)
                     /locus_tag="ACGI8A_000448"
     CDS             complement(56464..56781)
                     /locus_tag="ACGI8A_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178460.1"
                     /translation="MERKKHEFTDQDIQRLIEGLRTDPKIREMIFKSKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            56919..57107
                     /locus_tag="ACGI8A_000449"
     CDS             56919..57107
                     /locus_tag="ACGI8A_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178461.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            complement(57100..57360)
                     /locus_tag="ACGI8A_000450"
     CDS             complement(57100..57360)
                     /locus_tag="ACGI8A_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178462.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            57529..57735
                     /locus_tag="ACGI8A_000451"
     CDS             57529..57735
                     /locus_tag="ACGI8A_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178463.1"
                     /translation="MCKIKTLREKCSLIDDKVEFSGEYKTFPGFTGSKLLECECNKNY
                     ICKIQIMTANSNCPLFKKARQLQY"
     gene            complement(57754..57972)
                     /locus_tag="ACGI8A_000452"
     CDS             complement(57754..57972)
                     /locus_tag="ACGI8A_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178464.1"
                     /translation="MKDEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(57998..58183)
                     /locus_tag="ACGI8A_000453"
     CDS             complement(57998..58183)
                     /locus_tag="ACGI8A_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178465.1"
                     /translation="MNEKEETVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFDIKSDI"
     gene            complement(58211..58942)
                     /locus_tag="ACGI8A_000454"
     CDS             complement(58211..58942)
                     /locus_tag="ACGI8A_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0178466.1"
                     /translation="MDLLKDARKKEWFWLENDLIDREDLSIYEKMIYIVLARYSNEES
                     SCFPSYKTISEKAGCSIRQVSNILNELENKELIIRENRNHEGKKEKNSNLYFLISSKA
                     KVQNEVPNVRHEVPNPMAPGALHVRHEVPSKKTYNKKTYIKSNTTTQKESKTDYLDLS
                     FLDLDIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIINGKGSRYKSHYRALLTWG
                     NGDASKGIVPEVTKTKNPLSGFKEL"
     gene            complement(58970..59185)
                     /locus_tag="ACGI8A_000455"
     CDS             complement(58970..59185)
                     /locus_tag="ACGI8A_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178467.1"
                     /translation="MKISDFNKNQIGSAVQLQDIRFDTKADCIITKVEKNEILVMYYE
                     KETEEIAYKTLTKEDLILDDYKLKLLS"
     gene            complement(59199..59342)
                     /locus_tag="ACGI8A_000456"
     CDS             complement(59199..59342)
                     /locus_tag="ACGI8A_000456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178468.1"
                     /translation="MTDINKIREEIYRKLELLGPAKLKLALTKVDILKEIQDIEDENN
                     IQN"
     gene            59490..59876
                     /locus_tag="ACGI8A_000457"
     CDS             59490..59876
                     /locus_tag="ACGI8A_000457"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0178469.1"
                     /translation="MIDEMKPENKRIRELRLNLNLSQEEFANKLNLKRNTISLIENGK
                     RNASERTLNDICKTWSISEEWLKHGVGEMFFIKELDEQFAHLVGELSKMENEVEREII
                     TKMFKLNKKYLTVFDKMIDSLLEIEE"
     gene            complement(59891..60013)
                     /locus_tag="ACGI8A_000458"
     CDS             complement(59891..60013)
                     /locus_tag="ACGI8A_000458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178470.1"
                     /translation="MMDLEKEKHEIIEIIKKLNIDKDKRNLLMIKNYILGLLKK"
     gene            60590..60973
                     /locus_tag="ACGI8A_000459"
     CDS             60590..60973
                     /locus_tag="ACGI8A_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0178471.1"
                     /translation="MKIKKIPESELLVMMFIWDSEDKEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHKNSFESFISAL
                     HDDEEVSENKLKSLEKFIEDWEEDK"
     gene            complement(61122..61811)
                     /locus_tag="ACGI8A_000460"
     CDS             complement(61122..61811)
                     /locus_tag="ACGI8A_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFH0178472.1"
                     /translation="MLENRKQIQKEIYLPIATYCLKDKQYYIKEYGALLIKSAENSDI
                     FNTHKNRILYKNKLDIQEIANELNVNKATIQRNIKKLEKLDFKILKIENTLNGIVYCL
                     PSENNIDLNKFALINYEMLKKMVNKFKSNTIKVYFLLKTITTETNFKPATNSFIAENI
                     GLSSKSKNNLDIITSSVKTLEENKYIETKKVNVYEYDKEKLREVPKIRKVYRICNFDE
                     WKKEIDKNKVY"
     gene            62164..63351
                     /locus_tag="ACGI8A_000461"
     CDS             62164..63351
                     /locus_tag="ACGI8A_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFH0178473.1"
                     /translation="MEKKEILMQPNNLIKSKYDFTNVENKLFYKILFNAQKQQNSSYV
                     TTISKEELKVFMKNNNDYEHKNIKEILNMFQQSVLEFDYIEETTGKLKTFGSGLINTY
                     ELDHTDQIYTIMMHEVLYNHITDFVKMQKKKNGYTAINLSVLFNFRGAYTQRLYTLFR
                     LWSRENKEVEIKYKLDELRFYLKLKDNVYPEYKYFKQNVLKRAMNEINKKGNMVVSIK
                     EEKRKNRKIDEIIFSVIDYEPRKYFDKDILVEDYIPKKKQDNEVSFSFYIPNENIFTT
                     GTIKLFKEDFKEFDFKKDVYLNAFNKSIAIAFERDNTDIINTKNYDFFKSTLSNKISE
                     VIIHQKDDLKFKEELDKYWDDEDVKKEEREVDYSKDPVRLETVRRNLFDQGLVNEEGY
                     YYK"
     gene            63371..64105
                     /locus_tag="ACGI8A_000462"
     CDS             63371..64105
                     /locus_tag="ACGI8A_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFH0178474.1"
                     /translation="MYYVYKYINLDTEECLYVGKTENICDRHASHLSNKKENWCNKNL
                     RFEYIELDNKYTMDFYEIYLINKLEPKFNITGKGEMDIAKTSFSYNGQWVIYSERDLM
                     SSLASKRCGINYEVNAKMLGIMRKLKKISSNEEIFIGNENIRIKYYFETVLEGINLEP
                     CILSVAYKTLKESTVGTVISVKREYLSENVCLIIDSIFLNEIMKDPLMSKSDIKDLNS
                     QVNDILKIIGVDCEWNKLSDYCSANS"
     gene            64225..64377
                     /locus_tag="ACGI8A_000463"
     CDS             64225..64377
                     /locus_tag="ACGI8A_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178475.1"
                     /translation="MDNFLFNVLASLTASVVVYLISELFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            64765..65067
                     /locus_tag="ACGI8A_000464"
     CDS             64765..65067
                     /locus_tag="ACGI8A_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178476.1"
                     /translation="MEVNLHTGKDEFFKSHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            65102..65560
                     /locus_tag="ACGI8A_000465"
     CDS             65102..65560
                     /locus_tag="ACGI8A_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178477.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSVVVPGGTANLTAMSGGQLSWRVTSTLGNILGFEGNIDVYKAGKIVSSQYVG
                     FRLAGTTKTGVVTFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
BASE COUNT        22517 a         9874 c         8651 g        24705 t
ORIGIN      
        1 caagaagaat aataaattat aaaaatggag gataatacta tgaaaggaat attaaaagaa
       61 ctaacagcaa aactttatag ctttttttac tttatgttct ggggctttta ttttagcgct
      121 ggctatttct tttacaaaaa tatatgtact tcctttctgg tgaggcctta ttttagtgat
      181 tttgagcata agtaaatttt ataaaaatga ttattttata tatttatata agatttagtg
      241 cttgatagat tattttagag aactcattag tgagttctct tttttatttt aaatataaaa
      301 ttataatatt gggaggtagt aaaaatgata gtagtattaa aaatgggagc agataaaaat
      361 gaagttaaaa aattaattga ggctattggg agagaaggag tagaagttaa tcccatagat
      421 ggaacagaat taactgtttt aggtttagtt ggagatacta gtaaaataga tgccaaaaga
      481 atagaagcaa ataaggtagt tgaaaaagtt atgcatgtgg tagagccttt taaaaaggcc
      541 aatagaaaat ttcatccaga accttcaatc ataaatgtta atggaatgga aattggttca
      601 aagaaaattg ctatgatagc tgggccttgt tctgttgaaa cagaagacca aatagtatct
      661 atagctaaag atgtgaaaaa atcaggtgca ggatttttga gaggtggagc attcaaacca
      721 agaacatcac catacgcttt ccaaggattg aaatatgatg gattggattt attaaaaaaa
      781 gcaaaagaaa aaacagggct tcctatagtt acagaaataa tgtcaacaca agatatagat
      841 atatttgaag aaaatgtaga tgtaattcaa gttggagcta gaaacatgca aaactttgat
      901 ttattaaaag aattaggtaa aacaaataaa acaatacttt taaaaagagg attatctgct
      961 acaattgaag agtggctaat gtcagcagag tatataatgg ctggaggaaa tgaaaatgta
     1021 gttctttgtg aaagaggtat aagaactttt gaaacatata ctagaaatac tttggattta
     1081 agtgcaattt tagctgtgaa gaaacttagt catttaccag ttatagttga cccaagtcat
     1141 gcagcaggta aatcatggat ggttgactct ttatctaagg cagcaattgc agttggagca
     1201 gatggtctaa taatagaagt tcataatgac ccagcacatg cactttgtga tggaaaacaa
     1261 tcgattaagc caaatgaata tgatgaattg ataagtgaat taaagactat cgcatcagca
     1321 gttggtagag aaatataatc tataaaagga tggttaaacg tggaagaaaa actattagta
     1381 aatttaaaag aaaatagcta caatatattt attaaaaaaa atatactaaa gaatatagga
     1441 gaagaaataa aaaaaatata ttttggaaat aagattttta ttgtaactga tgaaaatata
     1501 gcaaaattat acttggataa agtaaaaaat aatttaagtg aatctggatt tgaaattaaa
     1561 tctttaatat taaaatctgg tgaagaaaca aaatcattta atactcttcc taaaatatac
     1621 gatagtttac ttgactttaa gttaactaga aaagatttga ttattacttt aggaggaggg
     1681 gtaataggtg atttaggtgg atttgcagca gcaacttttt taagaggtat aagttttatt
     1741 caaattccaa catctttatt agcacaagtt gatagttctg taggtggaaa agttggagtt
     1801 gaccttgaga gaggcaaaaa cttagtggga agcttttacc aaccaaaggc tgtgtttatc
     1861 gacccaaatg tattattatc acttccagaa aaattctata gagatggaat ggcagaagtt
     1921 ataaaatatg gatgtataaa agacaaaact tttttcaata aacttttaag tttaaaaaat
     1981 agagaagaag taatgcaaca tatagaatac attatacata aatgttgctt tattaaaaaa
     2041 actgtagttg aagctgatga aaaagacttt ggagatagaa tgcttctaaa ctttggccat
     2101 acattaggtc atgctattga gaaatactac aattttacag gttataccca tggagaagct
     2161 gttgccatag gaatgtataa gataacttta gaaagtgaaa agataggaat tacaaaaaaa
     2221 ggtacatcag aacttataaa atcaatttta atgaattata atttgcctta tgagattgaa
     2281 ttaagagata ataaatctat agtagatgct atatctcttg ataaaaaaaa tctaggtgat
     2341 acattaaaaa taatactttt aaaagatatt ggagagagta ttatatataa tactacttct
     2401 aaattttttc aggaggtaag ataatgggaa gtctaaaaat atatccaagt aaattatctg
     2461 gagatgtaaa aataccacca tcaaaaagta tggcacatag ggcagttatt tgttcatctc
     2521 taagcaatgg aaaaagtaga atttctaata tagatttttc tgatgatata atagctacta
     2581 taagagcaat gacatcatta ggagctatta tagaaaagaa agaagatatt ttagaaattt
     2641 cgggaatttt ttctaaagaa ggtattttaa atagagagaa tcagttgaat caacctaaat
     2701 tgacaataga ttgtaatgaa tcaggctcta cattgagatt tttagtacct atatctttaa
     2761 catttgatgg tgtaaaaagg tttataggta gaggtaatct tggaaaaaga ccacttgata
     2821 cctattatga gatatttgac agacagaata taaaatatag ttacaaagaa aatcagttag
     2881 atttgattat atcaggtaag cttaaaccag atgaatttag agtaaaagga aatataagtt
     2941 ctcagtttat cacaggttta ctgtttatat tgcctacgct agaaagtgat tcaaagataa
     3001 taattactac tgagctagaa tctaaaggat atttagattt aactctttca acgataaaag
     3061 attttggggt agaaattata aacaataact ataaagaatt tattataaaa ggaaatcaaa
     3121 cgtataaagc gagagattat aaagtagaag gagattattc tcaaggagca ttttatttat
     3181 cagcagatgc tataggagaa gatattagca tattagacct taaagaagat tctcttcaag
     3241 gggatagtga agtagttgaa attttatcta ggatgggaat ggaaatatta agagaaggca
     3301 acaaaataaa aggaataaca aatggtttaa atggtacttt gatagatgca tctcaatgcc
     3361 cagatataat acctgtatta tctgtagtag catccttaag tatagggagg actacaataa
     3421 ttaatgctgg taggcttaga attaaagaat gtgatagact tcatgcaata aatgttgagt
     3481 taagtaaact tggagcaaat atagaggaga aagaggatag tttgataatc gaaggagtaa
     3541 gcaagttaaa tggtggagta gaggtgtgga gtcacaaaga ccatagaatt gctatgacac
     3601 ttgcaattgc ttcttgtaga tgtgataaac caataatatt aaaagatttt gagtgtgtat
     3661 caaaatctta tccacatttt tttaaagatt ttaagatgtt aggaggtcga attgatgagt
     3721 ggaatatggg gaaataattt aaaagtatca atttttggag aatctcatgg aaatgctata
     3781 ggtataaata tagatggtct tccttcgggg attgaacttg accttgataa tattgataaa
     3841 gaaatgaaga gaagagcacc tggaaaaaat tctatatcta cttcaagaaa tgaaagtgat
     3901 ataccagaaa ttctaagtgg atattttaat ggaagaacta caggaacgcc attatgtgca
     3961 atcataagaa atagtgatac tcgttcaaaa gattatggag agcttaaaaa tttaatgaga
     4021 ccaggacatg cagatttcac aggtaatgtg agatattctg gatttaatga ctatagaggg
     4081 ggaggtcatt tttcaggtag gataacagct ccattagtat tttgtggggc tatatgtaaa
     4141 caaattttat cacaaaaagg aatagagata ggagcacaca taaaaaaaat taaaaatata
     4201 gaagatatga gttttgatta tgtaaatatt tcaaaacaac agttatcaaa tttgcaaact
     4261 ttggaactac ctttacttga tttatcaaag gaagaagcca tgaaaaatac gattatagat
     4321 gcaaaaaatc aaggagattc agttggtggt ataattgagt gtgcagtagt aggtattaat
     4381 gtagggcttg gaaatccatt ttttgactct gttgaatcta ctttatcaca tttattgttt
     4441 tctgttcctg ctgtaaaagg tgtagagttt ggtttaggtt ttgaacttgc agatatgtat
     4501 gggtctcaaa gtaatgatga aatgtattat gaaggaaatc aagttaagag caaaacaaat
     4561 aacaatggag gaattatagg agggattact actggaatgc caataatttt caaagtggca
     4621 attaagccaa ctccatcaat tagcagacaa caaaacacag ttaatataaa agataaaaag
     4681 gacgatattt tatatattaa aggtagacat gacccttgta tagttcaaag ggctatacct
     4741 gtaatagaag cagtaacagc cattggaatt tttgatttga tgaaaggacg ataataatgg
     4801 aggatttaac gagatataga aatgagattg acaatataga taaagagctt attcaattat
     4861 ttgaaaagag aatgaataca gttcttgaaa ttgccagaca taaaatgaaa aataacacta
     4921 ctatacttca aaaagataga gaagaaaaag tactaagtag agctgtagat aatttagata
     4981 ataaagcata ttcacaagaa acggtacagt tttttaattc tataatggaa attagtagag
     5041 gattacaaaa aagattgata gataatgatg gtgagcagaa aatcgagttt agtttagatt
     5101 catcaaataa gaaatttgat ttgtcaaatg taaatagata taagtcttta aaagatgaat
     5161 taaataaaaa aaatatccta gtaggatttc caggaaaatc aggttcattt acagaagaag
     5221 cactaaataa attcttcaac aaaaaaatat caaaaaaaca atttaaagaa tttgaagatg
     5281 ttttcatagc acttaaaaat aaagaaataa actatggaat aatccccata gaaaattcat
     5341 ctacaggagc tatctctgaa acttatgacc ttttgagaaa gtacggtttt tatatagttg
     5401 gagaagaatg tataaaaata gaccaaaatt taatagggat aaaagacaca aagcttgaag
     5461 atataaaaga aatatattct catccacaag gtataggaca atgtagtaaa tttttaaaac
     5521 aaaatagtac atggaaattg ataccattta ataacacagc aacaagtgca gaacttgtaa
     5581 gaaaacttca agataaaaca aaagcagcaa ttgcaagcaa aaaagctgca agtatttatg
     5641 gattagaaat gataagtcct tgtataaatg atattgcaaa caactataca agatttgtag
     5701 taatcagtaa tcaaatacac attgaagaag aatcaaacaa aatgagtgta gtattttctg
     5761 ttgaacatga agcaggaaaa ctttataaag tacttggata ctttgctaaa aataatataa
     5821 atatgacaaa aatagaatca agacctatga aaaatgcctc atggagatat ttcttttata
     5881 tagattttga gtgttctata tacaatgcta aagtgtacga tttattagaa ttgatagaac
     5941 ataatacagc atattttaaa tttatgggag tatataaaaa taaaactgaa tagataagat
     6001 aaatttttat ttaatttata attattagga ggtgtaagta tgaatttttt cggtctagta
     6061 ggtgaaaaac tctctcacag tgtttcacct caaatccaca agagagtatt cgagatattg
     6121 aatattgaaa gtgcttataa aaactttgaa atttcaaaag aagatatatc taagctagac
     6181 ggtgctataa aattattagg aatacaaggg gtaaatgtta ctgtaccata taaggagcgt
     6241 attatgaaat atcttgattt tatatcacca gaggcaaaac gtataggagc agttaataca
     6301 attttactta gagaaaatat gttatatggt tacaatacag attactttgg tcttgatagt
     6361 atgtttaaaa tggctaatat tgatgttcaa ggaaaagtag cagttatatt aggaactgga
     6421 ggtgcttcta aagctgctct tacatatttt attgattctg gtatagaaaa gctttatgtt
     6481 tcaactagaa aaaaggatga taaaaaatta ttaaatagta aagctatatt aatagattat
     6541 gaagaactta aacatataaa aggagatata atattaaatg ctacaccagt aggtatgtac
     6601 cctaatgtag gaatttcacc agtgtcaaaa tctattatac aaaattttga tatattaata
     6661 gatttaattt acaatcctgg agaaacagag tttttaagaa ttggtaatag tatgggtaaa
     6721 aaaacttgtg atggattata tatgctggtg gggcaagcaa taaaatctca ggaaatttgg
     6781 caagatacta aaatagataa tagtatttta gatgttatat ataatgaact aaaattagaa
     6841 tttttgtaag ggagattgtt atgataaata aaacaaaaga aaaattgata ttaataggta
     6901 tgccaggtag tggaaaaact acaattggta aacttttggc caaagaatat aattgtagtt
     6961 tctgtgatat ggatgattat ataattcaaa tatctcaaaa aagtatagca gagttgtttt
     7021 cagaaggaga agatatcttt aggaactatg agactcaagc atgtagagaa ctttctataa
     7081 gtgataaaac agtgatatct acaggtggag gtgtaataaa aaaggatgtt aatatggaaa
     7141 tactaaaaga aacaggtata ataattttta ttgatagacc tatacaaaag attttggaag
     7201 atataaatat taattcaaga ccattattaa aaaacggaaa agatagattg tataatcttt
     7261 ataatgagag aataaatctt tataaaaaat tttcagatat agagatttta aatgataaat
     7321 cattaaataa tgctgtatat aatattacaa atgcagtcag tgaaaatttt aaatttgact
     7381 ttaaagaaaa ataatctagg gggaaataaa aataatgaat attgttattg taggactagg
     7441 agttatagga ggctcttttg ctaaagcatt aaaaaaagct ggatatgaaa atgtatttgg
     7501 agtagatgta gatttagaaa ctttaaaaaa agcagaaaaa gcaaaaatca taaaaaaagg
     7561 atgtactaca ggtaaagagt tatttaaaaa agctgattta attatattat ctatatatcc
     7621 tagattagtt gtagattttc taaataataa taaaaacttt tttaaaaaag ggactataat
     7681 tactgatact acaggaataa aagaaacact tatcaatgat gtgttgcaaa ttattccaga
     7741 tgatatagat tttatatttg gacatccaat ggcaggtagg gaaaaaaagg gtattgattt
     7801 tgcaagtgaa caagttttta atggcgcaaa ttatataatt actccaactg gcagaaacaa
     7861 cataaaaaat ttagagttag ttgaaaattt aattttagag attggattta aaagagtaaa
     7921 aaagttaact tcacaaaaac atgatgagat tatagctttt acttctcagt tacctcatgt
     7981 tatggcagtt gctcttataa atagtgatga agaaggtaga gatacgggta aatttattgg
     8041 agatagttat agagatttaa ctagaattgc aaatatgaat gaagacttat ggagtgaact
     8101 ttttttagga aatagagata atttactaaa agttatagaa aactttgaat cagaagtaaa
     8161 tttagtaaaa gaagctatat ttaacaatga taaaaataaa ttaatagagt attttaaaaa
     8221 atcatcaata agaagagaag ctttagaaaa ataaatatta atatttaagc tatatataga
     8281 tgggtataaa tataagcgat agtgtattta attgtattaa taaatatgga ttcaatataa
     8341 ttaaatatgc tattgatata tttgtaccct taactattgt tatcttttaa atgataatga
     8401 tatttattta aatactcttt attaacaatt tataaaactt aattgtaaaa aatacttatt
     8461 aatagtaaaa taaagtgaat caattatatt ataattgcta aagagagtga ggtgtagcta
     8521 tttgaaaaaa aatagattaa tagcaaagaa tatatcaata gcatttatag tactattctt
     8581 ttttagtgta tttacatttt tttatgtagg taatattaat agagtgttag aatatgaaac
     8641 aaatgacata ataacagtta caattgctgg atggattata ctttcatttt tatttttagg
     8701 tattataatt tacatattat attcaaaagc aaatagtcaa aaaactatag aaaaagtagc
     8761 atatacggat tttgtaacag gttattccaa ctggagaaag tttgaattag atgttacaaa
     8821 tttattaaag aaaacatcac aaaataataa atatgcaatg gtcatattcg atattgataa
     8881 atttaaagca attaatgata tatatggcca taaaaaggga aatttaatat taaaagatat
     8941 tgcagatact ttaaatgaat tgactgatat caatgaaact tttgctcgtg taagtgctga
     9001 taatttcaat atattattaa catataacaa aaaagaggat ataataaata ttatcaaaaa
     9061 gattatggca aataatgaac ttgtaaactt atcttttgga atttatgaaa tcaaagataa
     9121 ggatttatct gtaagtgtat atagtgatag agcttcactt gcaaaatcat caataaaaaa
     9181 taatagtgat gtaaattttg cattttttaa tgacaaatta agagagaaat tattatttga
     9241 agataagata gaaaaagaaa tggaatatgc tcttgaaagt gggcaattcg taatgtattt
     9301 acaaccaaaa tacaatatta aattagataa attttgtgga agtgaagccc tagtcagatg
     9361 gcaatataca gaaaaagagg tcatatatcc aggtgatttt attcctatat ttgaaaaaaa
     9421 tggatttatt agaaagattg atatgtacat attagaacaa gcttgtaaag aaatccggtc
     9481 tttatttgat aaaggaattt cacctttacc tatatctgta aacttctcaa gagttgattt
     9541 ttttaaaaaa gattttattg aaaacatagt aaatatatgt gatagatata aaataccata
     9601 tagtttaata gaaattgaaa ttactgaatc tagtatgttt ggagatactg atactctatt
     9661 taatgttagt agaaacctac aggatattgg ctttatagtt gcaatggatg attttggctc
     9721 tggatattct tctgtaaata tgcttaaaaa tataccatta aatgtaataa aattagatag
     9781 aggatttttt gttgatgata aagatgttga taagagtcaa attgttataa aaagcatagt
     9841 ttctctcatt aagcaattag gaataagggt tgttgctgaa ggaattgaaa caagaagtca
     9901 aatagaaatg ttaaaaaaag caaattgtga tattgttcaa ggatattatt tttcaaaacc
     9961 attaccaatt aaagaatttg aaaaattagt ctataaaata tagtcagcga gtcttaaatg
    10021 ggaggtggtg attaagtgat aagagcaaaa tcgttaaaaa atgtactgag tattgtttta
    10081 atatttttat ttacaataat ttctttttct gattttgcat ttaaaactaa tgtccaggaa
    10141 gaagaaaata gtaaaattaa tttaagagaa atatccctac aaagttccaa tgctataaaa
    10201 ataaaattag atagttctct atcctttatt aaaagtgtgg caaatatggt ttcttatttt
    10261 aatactatag ataactcaaa aatagttcca ttattagcta atatatcaaa agaatctgac
    10321 tttcaaagaa tctctattgt atctcctgat ggaataagtc ataccagtga tggcaaaatt
    10381 atcaatgtgt caaagtatca gtattttaag aaagctatta aaggagaaag taatatatct
    10441 gaagttgtga aatctccaat tgatggaaaa gatacttttg ttatagctac tcctgtattt
    10501 aataagaata acaaaataat tggtgtttta tacggttctt actacaatga taaattagct
    10561 gaatttatag atataacaag ttttaatagt gaagggtaca tagatatatt tgaatctagt
    10621 ggtcaatttg ttttaaaatc ttcacataca aacaaactga ttaagtcaag tagtaacata
    10681 tatgatatat ttaaaaaagc ttcttttgat gatggttata gctataaaaa aatatatgaa
    10741 gatacaaaaa ataacaagag tggctttacc tcatatgaat acaaaggaga aaatcgttat
    10801 gctacttata cacccattgg tgtaaatgac tggtatgtaa tttctgtgat accacaatat
    10861 actgtaagaa gaaacattga taaaataaat caattagcat tttctcttat tttcaaggta
    10921 ttcttattgt ttattatgct tataatatat attatttata aaaacaataa atctcagaaa
    10981 gatatactaa aagctaacaa agaaatctca tctattacaa atagcattcc tggaggagta
    11041 caaaagtgta ccctgggtga aacatgcaat attaattttt taagtgatgg atttatagag
    11101 ttgtttggtt atacaagaga agaaataaaa acattgttta ataatgattt ttatagttta
    11161 atacataaag atgatgttga aagaataaaa aaagaaattt ttaaacaact ttacaaaaat
    11221 aaaactgttg aattagaata taggattata acaaaaaatg gtaatacagt atgggtttta
    11281 gataaagggg agcttataac agaacctgat ggaaaagaat atttttgttg tgtaactgta
    11341 gatattacta gaacaaaaca attagaacaa gagatggaac aaaaaaatct agaacttgaa
    11401 atgatatcag aaaatgtttt aggtggtata attataacag aatttaatga tgattttact
    11461 ataaaatacg ctaactctgg ttatctttca atgattggat atacacgtga acaattagaa
    11521 aatgaactta aatacaaagc ttgtaatctt gttttttatg aggataaaaa gaaggctctt
    11581 gaaagtatac aacatcaatt aaacacaaaa ggaatagctg tatatcaaca tagaataaga
    11641 agaagaaatg gtaatattat ttgggtttct ataaaaggaa ggcttattgt tgatgatggt
    11701 attgaaaaag gtatttggat tctaaatgat ataacagatt taaaaaatgc agaacaaaaa
    11761 cttaaaataa atgaagaaag atttagaatt gcattaaagc aaacatcaaa cactatattt
    11821 gagtatgata taaaaacaaa gtcactttta aatactagta aagagcttga taaatataat
    11881 atttataaaa ttactaaaaa cgttccatat tctctagtag aatcaggagt tattcataaa
    11941 gattttagtg atgattttat aaacatgtat gaaaatgtaa taaatggagc aaaaaaagca
    12001 agttgtgtag ttaagatgaa acttccagat gaaaattata tatggaataa agttaccttg
    12061 actactattt ttgatgaaga tggtaatcct gtaagagcaa ttggtataca ggaagatata
    12121 agcaaacaaa aggaagcaga gataagatac aatcaagaag gtaagtatca ttcagctata
    12181 ttgtcggaag caatgataac ttatgaaata aatgtatcaa aggatacttt tatcaaagga
    12241 aatgaaagtt ggcaaaaacc atttggaatt aagcctgaca ataactattc tcaaatggta
    12301 aaaactatag caaatagtat tgtacataaa gacaataaag aaacattttt aaatacattt
    12361 ttatgtgaaa acttattgga tgcatataaa aataatgtga ctgaaataaa attagaatat
    12421 cgaagaatga atcaagaaaa taaaatgatt tgggtcattt gtacaatgca cttaataaaa
    12481 gattctatta cgaacgatat aaaagcactt gcttatgtta aagatattga caagcaaaaa
    12541 aatgaagaaa ttagattaaa attaaaagct gaaagagata ctcttacagg attatataat
    12601 aaaggtacta cagaaaaatt gattaatgat ttcttgaact ctgaggaagc aagcttagga
    12661 atgcatgcat ttttaattgt tgatatagat aattttaaat ctgtgaatga taatttagga
    12721 catgtatttg gagataaagt attatttgaa gtttctaaaa acctaaaacc tatatttaga
    12781 aaagatgata tagtaggtag aattggtgga gatgaattta tagtattctt aaaaaatatt
    12841 cagtcttttg agaatatcaa aaagaaagca gaaaaaatat gcgatatatt tagaaattct
    12901 tatacagaaa ataaaaagac atataaaata tctggaacag ttggaatttc atttgtacca
    12961 gaacatggaa ctgtatttga agaactttat aaaaaagcag acatagcact ttattatgca
    13021 aagagcaaag gaaaagataa gtatgcactt tataatgatt gtatctacca aaatgaactt
    13081 gaaaataata taagtgataa taatataaaa ttagcagata aatgtgagaa atccaaacca
    13141 gtacttaaag aaattcttga ttctattgat aattatatct atatagtgaa tccaaatact
    13201 tatgaacttc tttttgtcaa taataaggtg tctcgtttgg atttagagat aaaattagga
    13261 gaaacctgtt ataaatcact attaaaatct gatagaccat gtaataattg ccctcttaac
    13321 ggattagaag aaaataagca ttcaaaattc aaaatagaga caaatacatt tggtaaaaat
    13381 atcagtatag caagttggat taattgcata gatggagaat acaactgtct tatagagtgt
    13441 atagattctt ctttatacaa aaataaataa aaaaggacta tgaagatatg ttaaatagta
    13501 taactttgat acttggtata atatttacaa tttactattt ttatctcaaa atagtttttg
    13561 gaggaatttc atttagtgag attttcttag ttgtaggaat tgtactaatt atctatcaaa
    13621 tatttaagaa aaaaattaaa gaaaagaagg ttttttatag ggtgttaaaa ataataatca
    13681 gcattttttt aatagtattt gtagttactg agagtctaat tattttttat cccaaaaata
    13741 gtttagaatc aaaaagtgat tatctcttaa ttttaggagc ttctgtaaaa aagactacac
    13801 caagtacaac cctgaagggt aggttagaca ctgcattgaa atacctaaaa attaatgata
    13861 attgttatgt ggtagtatct ggtggtaaag gaagtggaga aaaaataact gaggcaaaag
    13921 ctatgaaaga ttatcttata aaaaatggta tagataaaaa taggataata gaagaggata
    13981 aatccacgaa tacttatgag aattttaaat attcaaaatt gaagatagaa gaacatagtc
    14041 aaggaaaact aagcgattta aaagtaaaaa tagtaacaac agattttcat gttctcagaa
    14101 gtaaaatatt agcatataga aatggatata aaaatacgtc tttttatgct agtaaatcaa
    14161 aactttcatt tgtaccaact tactatacaa gagaattttt tgcattatgg aaaacaatca
    14221 tttttgatag ataataaata aaaagactag atataaactc atatctagtc ttttctattc
    14281 ccttaattct aatccatttt ccaactttac ttgttgtaga aatttcaaca ttgggattta
    14341 caagtaattg cttataagaa gttttatatt ttacgattcc aaaatataat tttccttcat
    14401 aatccattat aaatccgaat ggtcttactc ttggtttatt atcttcaact gtagcaaaat
    14461 agaatgttga gttttcttta aaaaaattca ttattttttc atgattaaat cctctttgta
    14521 tcttatacta atataatgaa tataaataaa atattcataa tactaatata aatcgatata
    14581 aaaacaatta caatattata ttgattattc aaaattatat ataaaattca gaaaaaaatt
    14641 ttagtttaca taataaaaat aaagtcaact aaaattattg attttagatt aaagtaccgg
    14701 attaagcatt taagtagatt ttaagcttga tttgattaga aaggtacaaa ctatcaccga
    14761 aaaaaattaa acccatttaa aaagcaatat aaaagcttgt tatttttgag aaaaatagac
    14821 cctctaaact caattagatt aaaaataaaa tacagaaaat tatatctcta aatatcttaa
    14881 ataattaagt ttaaatttta cgatattttg tttgatgatt tataatataa aagcatactc
    14941 ttaatcttca aaatttaaaa agagtaaaaa ataacaaaaa aatcactgac atttcactga
    15001 caaattgtaa ttttgttatt ataaatacat tgaaaatact tgattattga aaaatggcat
    15061 aaaataatac attttatgcc atttgctatt atttgatagt ttttaactta tgacataaga
    15121 tattttttaa catatacaat aaaatataaa aaaattgata ttgcaaataa aaaaaaatac
    15181 atattagatg ttatagaaaa aaagcgcact tataggctta aaatgtcaca taaaagagtt
    15241 ttaaagaata atatattatg aacacaaatt ttgtaatcac aaaggagtga taatatggca
    15301 acagtatcag gacaaatagt ttttgataga aatagaagtg ctaccattga ttctggagac
    15361 tcaggaatag ccaatatacc agttgtatta caagatatag caactggagt tagacttgtg
    15421 gttctcacag atgcatcagg aaattatgtt tttactaatg ttccaaatgg taactataga
    15481 attgtagaag cttttggaac tacaggagga gtaccaacac caggaaattt tgcaacagcg
    15541 atagtaggtc cagttccaat tgcagaaatg ccacctatta gttctgtgac aagtcctcct
    15601 attggttcaa caaacttaga tggtacaaca cctaacacac tattaataac agtagcaggt
    15661 tcagatataa caaatcaaaa tatattaaat gggcctgtga tttatacacc tatagagaat
    15721 atattggatt cttgcgtatc tgtatctaat ataaacctta ttacagatgc agataacgga
    15781 acattcggtt tttttccacc aggtacacca agtaatacag gacctgaaat agaaccttac
    15841 ccaaatgtga cgccagattt tacgtatgtt gtaccagacc caactaagtt tacaccttta
    15901 gatggagaat atacagtaca aaatattatg acaaatgcaa tgagtaatgt tataggagct
    15961 tggtggcgta ttgctgacca tactacagga aatgaaacag gaagaatgat ggtggtaaat
    16021 ggttttaatc ctggagctgt attttttaaa tcaacagtac aagttacacc taacactaat
    16081 tatttattta gcacttggat tttaaacctt tttaaggtaa caggatatcc accaccacag
    16141 ctaggtgtaa gaatatttga ccaaaataat aatatactat atcaagcaac attaggagct
    16201 caaattccag ttaatacaaa cgcaccagaa tggaaagaag taggtactgt gattaattct
    16261 caaaataata atcaacttac agtagaattt tttagtgaag gagaaagcgc cattggaaat
    16321 gactatgcta tagatgatgt ttctttaaga gaaatagctg ttccaacttt tactccaata
    16381 aaaacaagta acacagaaac tgctaatgta ggtgatataa tcacatatac agttacatta
    16441 gataatactt gtgatagccc tttaactaat gtatttttta gggatttaat tccaaatggt
    16501 ttagagttta tacctggaac tgtaacggtt gatggagtat ctgtaccagg agtagacccc
    16561 aatacaggtt tcccacttcc agatattgga ggaggaacag gggttgaagt aacttttgat
    16621 gttgtggttg aaagtattcc taacccaaat cctactaata atatagctac tatagattac
    16681 tcctatacac ctgtagaagg cggtatacca aatgattttt cagtagattc taatccagtt
    16741 cctgttgaag taatctcagc agatattgaa gttacaaaat tatctgaacc tactatagtg
    16801 aatccaggag aggaattaat atacactata aaagtagtaa ataatggacc attcccatct
    16861 gaaaatgtgg ttttaacaga tgatgtacca gcttctattg taaatccaga gtattcatta
    16921 gatggaggag taacttttca accatggact ggaagtttaa atatagggac attagaagta
    16981 ggagagacaa gagttattat aattaggggt attgtaaatc catcaacagt aggaattata
    17041 acaaatacag ctgtagtatc ttcaactaca gcagacccaa accttaataa taatacatct
    17101 acaatagaga cagaagtgaa tttattagca gatattttag taatgaaaac agcagaacct
    17161 aattcagctg ttccaggtac tttattgaga tatactattc aagtagaaaa cttaggacca
    17221 gcaaatgctg aaaatgtaat attaaatgat gatattccag cttctattat aaatccagaa
    17281 tattcattgg atggaggagc aagttttcaa ccatggaatg gaagtcttaa tataggaact
    17341 ttaaactctg gaatttcatt aacagtttta attcaaggaa cagttagttt aaattcttca
    17401 gaatatatag taaatacagc aacagtttct tcaactacac ctgacccaga tttaagcaac
    17461 aatatatcta ccattattac accagttaac ccacaagcag gtatttctat tataaaagta
    17521 gctgacgagg atgtagctgt tccaggagaa gaatttgtat atacaattga aatttttaat
    17581 gaaggaccat ctaatgctac gaatgtagtt ttaacagatg atataccaga tgttatttta
    17641 aatccagaat attcattgga tggaggagca actttccaac catggactgg aagtcttaat
    17701 ataggtacag ttgcaccagg gcaattaata agaattatca taaagggatt agtaagttca
    17761 actgctacag gagatattac taatacagca gaagtaagtg cagatgtacc agaaccagtt
    17821 acggattcat caacagtgac aactcctata gttccatcag cagatataga agtaataaaa
    17881 actagcaata tggatacagc agttccagga gaaactttca gttatactat aacagttaca
    17941 aatttaggac catctgctgc acaaagtatc gttttgacag atgatatacc agatgttatt
    18001 ttaaatccag agtattcatt ggatggagga gtaactttcc aaccatggaa tggaaatctt
    18061 agtataggca ctttggatgc tggagaaata agaagtatta taataagagg aacagtgagc
    18121 cagacagcag taggtacaat tataaataca gcaacaataa gttcaactac acctgaccca
    18181 aatccagata ataatacttc aacagatgaa acagatataa gtccattagc agatatttct
    18241 gtgataaaag gcaacgaacc agttgctata ccagggggaa gatttattta tggaattgaa
    18301 attgcaaatg caggaccatc ttttgcagag aacgtaactt taacagataa tataccagct
    18361 tctattttaa atccagagta ctcaatagat aatggagtga ctttccaacc atggaatgga
    18421 agccttaata taggtacttt agatgctgga gagataagaa atatcataat aaggggaaca
    18481 gtaagtcaaa cagcaatagg cacaattata aatacagcaa cagtaagttc aactacacct
    18541 gacccaaatc taaataacaa tatttcaaca tcagaagcag aggtaagttc atcagcagat
    18601 atttctgtag taaaacgaag caatcagact gttgttgtac caggagatgt actagactat
    18661 acaattgagg tgagaaatgc aggaccgtct actgctcaga acgtaacttt aacagataat
    18721 ataccagctt ctattttaag tccagaatat tcaatagata atggagtgac ttttcaacca
    18781 tggaatggaa gtcttagtat aggcacttta gatgctggag agataagaaa tattataata
    18841 aggggaatag taagtcaaac agcaataggt acaattataa atacagcaac agtaagttca
    18901 actacacctg acccaaatcc agataataat acttcaacat cagaaataga tataagttca
    18961 tcagcagata tttctgtaat aaaattagca aataaaacag aagcatgtgt aggagagcaa
    19021 atagattttg taatagtagt atcaaatgca ggaccagaaa atgctcaaaa tgttacttta
    19081 atagacaatg tttcagataa gcttaagaaa gcagtatttt cacttgatag aggtgtaagt
    19141 ttccaaccat ggactggaag tcttaatata ggtacactac cagctggaac tttaagagtg
    19201 attatattaa gaggaattat aaaatcaaca tgtcttgata gattaacaaa tattgcagaa
    19261 attacttcaa caacaccaga ttcaaacctt aacaataata tatcaagagt gcaagtagaa
    19321 ataaaacaat gttgttgtaa tgattgctgt tgtaacaact gtagtaattg ttgttgcaat
    19381 gattgtggtt gtaaagattg ttgctgtaat tgttgtaaaa atgacttttg gtgttgattt
    19441 tataaaataa ttttgtatac cttaaattaa actgaaataa aaaataaatt cacatatgag
    19501 taaaatattt tataaatatt aataaatttt ataatatttt ataaaaaagg aagtggttga
    19561 atgagtagga tagttctaac atttaaaaac aatgataaag aaaaagcaat tgaaaagttt
    19621 ttagatgaga aattatcagc tacagcttac ttaaaagagc ttgtttggga aaaaatgcaa
    19681 gaaaaaaagg ataatgctgt agttgaacag aaaaaagaaa tagaagatcc agctaacaat
    19741 tttgattttg gaagtttaga ataataaggg ggaaataaca taaatgagta aattaggtat
    19801 agatattgga aattatgcag ttaaaacaag tacagatgat atttttgaaa gtaaagttac
    19861 agaggtaaaa aactttggtt cagattcaga tagcattaaa ataggcaata aaacatatta
    19921 cctaggtgaa ggtgacgagg aaataaatat agttaaatat gagaaagaaa acttcttacc
    19981 actactatta ggggctatat gtagaaatac agatgatgaa attgttgatc tagggctagg
    20041 tctgccagtg aaacaatttg ccggattaag aaaaaatctt attgaaaaat tacaaggaaa
    20101 agaatatcat gttgagttta caagaggaaa tgagactact aaaagagata taacaataag
    20161 atctattcaa acatttccag aaggtgttac aggatactta tattatgcaa aagacatagt
    20221 tgaccaaatt gcaggaagag atgttgtttt agttgatatt ggtggaaaga caacagatat
    20281 tgcgcttgta caagggaata aagcgactga tccatattct ataaatgtgg gaacaataaa
    20341 tatatatgat gcaataaaga aatctcttga gatggacgaa agatttttag gtaaagttga
    20401 gataaaaaga gaaaaaatac aagactacat aaataaagga ttttacctaa atggtgaaaa
    20461 acaagatata aagaaaaata tagatgcatc tatttctcta tttaaagaaa tatacaatga
    20521 attgaaatta aattatccag ttagtacagc tgcggttgtt gttatgggtg gaggcgctaa
    20581 attgttaggt gaagcattta aaaagaatat acctggcata atagttatga gtgatgtaga
    20641 taaacatgtt tttgcaaatg caaaaggata caaaaaaatg atgaaataaa aaaggattta
    20701 gtttatagta tgtataatag attacaaata tatgatattt gtaatctatt atttttttga
    20761 ggaggattta tatgacttgg agtgatttga aagaaaaaaa tattaatgaa gttgttgatt
    20821 gtattaataa taaattaagt gaatttgaaa gtcttaaaaa agttggagat gaattagggg
    20881 caaatgaaag tactataagg aagtggctta ataagaaagg atataaaaga actggaaaca
    20941 agtttgtaga agataataaa agttatgatg ataaatatca tatagatagc attagcagta
    21001 agattgatga cgatattaaa tatttatcta gtgaaattga taatataaag aatgtgctgg
    21061 aatggtttaa aactaaagat gatagttatc atatatgtgt cattaaagaa aatgatatat
    21121 ctatagatct tccagatcaa tctataaaaa gaactacagt tagaataaat aatacagtgt
    21181 gggaaatgtt taataaattt gcagatgaga ataaacatta tgataagcat gatttgttaa
    21241 gtcaattatt gcttgaaagt ctattaaaat atgtgaagag tagatgaagg gagatattta
    21301 atcttcttca tctagatttt taaatttatg tacagtggac attaatcgta taaaatattc
    21361 atctaaataa tctggctcta aatcatttat tttatatttg aaatattctt tttctgtaac
    21421 tataggtatg taatatatgt ggaaaatttt aattctctta agtagaactc tttttttaac
    21481 aagtctttta agtagagttt ttgtcgcaga tttccgccaa ttgtgttttt cattgcaaat
    21541 gcgaataatt tctttctttg atattcttga atttcttttc taaaagattt tcattacaat
    21601 taattcgcca cgttttaagc tttgtattaa cattatgcac tccttgtaaa tgtttataat
    21661 atagtagcat ttttttattt aagatagtac aggtaagtta ttccaaaaaa gctaatgcaa
    21721 tgcaaaatgc ggaaggaaaa acctaccaca ttttgcattg tattaatatt ctattttaat
    21781 ttctttgcta tttcttcaac aacatctaca gtaacactat tgcctgcttg cttgtatagt
    21841 tgagtttctg aacagacact tgccgctcta tcgtaataat cgtctggaaa tccttgtaac
    21901 ctaaaacatt cttttggagt caacctcctt actctaaatt ttttaccaat tacgccagca
    21961 ttacttgcag tagtcaaagt acaacttttt ttgtgctgaa ctcttccacg gccagtttta
    22021 ccttctatat gatctagtct tatcccatct cctatagatg ctttgatgta tcctttttta
    22081 gtattctctt taattaatat ctttgggctt tcaccttttc cagttgtaag tgtgggagaa
    22141 aagccttcgc tactatacac ttgtccgcct gcaccatttc cactaggatt gatattgcca
    22201 actctataaa gacctgtatt gttcccacct ttacttaata aacactttga taatccattt
    22261 gaattataaa tcctgtttga actgtgatta ggattattta attgctcaag agtagttttt
    22321 ctactcgttc ctgtgatagg aaatacttct cttgtacttc ttcttctaag atgtccaaca
    22381 atgtatattc tgtctctgtt ttggggaact ctgaagtttt tagaattgag attttgccat
    22441 tctgcattgt agccgatttc atccaactca atgagaagtt tggcgaagtc aaatcctcta
    22501 ttaacactaa gtagattttt aacgttctca ataagtaagt acttgggtct atcttcttct
    22561 ttaagttctc taataagttt tgtaactgag aaaaataaac ttgaacgatt tcctttgaat
    22621 ccaagtttgt tccctgcaat gctaatgtct tgacatggga atccaaaaca ccacacatct
    22681 gctttgggaa tattttctgc tctaatttct ctaatgtctt tttcaaacca ttcatcctcc
    22741 tttgggttat gcattgcttt gtaacttaag tttgcaaatt tatcgtattc gcaatggcct
    22801 acgcatttgt gtcctgctct ttccattgct agcctaaaac ctccaatgcc agcaaacaaa
    22861 tctaaaaatg ttaacatata atgctccttc tgagttgaaa attttattag ttttattaac
    22921 aattaatttt taaaggttgt cttttaattt ataatttttc ttttacaaat tctataactt
    22981 tatccattgt tgaccataca tcattaccat atagttgagt aaatttttct cctgtagtct
    23041 tactcatttc tttcatttta ttacaagtta ctccaccaat tacatataga ttttgtgttc
    23101 tatgtggttt atagtctttt atatctataa ctagataact ttcttttgct ctcttataat
    23161 ataatcctaa aatatctgct gatactctat catcaccact gtacactatt gtgtgtttat
    23221 acatttgctt cacctcatta ctttcaatac tcttatttaa tacaccttct acaattaact
    23281 tagccatacc ttcatatcca agtttcttag ccttctcata atcctccttg ttatcacaaa
    23341 agaaactttc aattagtatt gcagtgggtt tagaactgtt taaaatatat aaacttttat
    23401 ctaatttagc tcctctattt ttaaatactg tacctagttt cttacatatt ctagttgcat
    23461 actctagacc tttattactg taatatagaa cctctgagcc ttttccttgc ctatcacttg
    23521 catttaaatg tagttctatg agtaagtcat atcctccagc attaactcta ggtattttat
    23581 aagacttttc ctcactctta gttttaaact gcttttctgg gcatattatt acatctacct
    23641 tatgcccttc ttttctaaat atatctgcta atactggtgc aagagattta ttgtattgat
    23701 actcgttaac tactccatca gcagaagtac atgctccact ttttaaaata ctgtgtccta
    23761 ctgtaataca tattttcatt atttattttc ctcctttaac atttaattca tctgtcatag
    23821 tatctaataa actacctatt ttctctttta atcgtttagg aactggtaat ccacataaat
    23881 acatattttt taatatactt acactttcat atagaatgaa taaaatagag aaaaattcag
    23941 atattcctag atgatttagt cgtaagaaat caacccaatc ttgtggcaac ataaataaaa
    24001 agttaaactt tgtaagtatg tcaactactg ctagaaaaaa tatacatgct atcattgcaa
    24061 cttttcttat tcctccattt attccaaaag agctattaaa ctgatgtgtt tttattgctc
    24121 ttagacaacc tagcaatgta tcaaatgcta ttgctaatat tactaatttt ataaatatat
    24181 ttgttgctaa aaaaactatt gttacgttca tacttcctcc taattttgta ttaaaataag
    24241 acttagaaat ttatctaagt ctttgacaac taacggcact tagctgctat ataactatac
    24301 acaccattgt taactggatt tctaggtatc catccattgt cagtaaaagt tattccataa
    24361 cgattatgat taacatcaag atatttttct ccagttgtat aattataaaa aagatagaat
    24421 aaaggtgtgt atacacaata atatttttcc tctacagcat taatggcagt ataggaaata
    24481 atatctggtc taaaacctat atttagccca ttaggatatg caccagtata ataggtaaaa
    24541 gttccactag tatatttata tttagaattt aactgactta tagtattttt aaatcctgca
    24601 atataattag catactcttg aaatgtttgt tcggatgtag caggagagcc gatagcagtt
    24661 gcaattaact cttttccact atcggcactt tgaaaagcca aatctgctct atctatacac
    24721 tctttcaatg ctccctcgac attttcacta gtgaatttat tctctgtatc ttctatagtt
    24781 acattctttg cttctaatac aagatttcta actttactaa ctaattcttt aaaagtcatt
    24841 tagtcacctt ctttcaataa aaaagaacct atattgttgg ttctactggt gtttcttctt
    24901 tatttaataa acctgttaac tctaaatatt gttcttctgt aatcctattt actgcataga
    24961 atacatccat cttatgttgt aaatcctcct tagtgctata gtttttttgt tctatcatta
    25021 actttaataa gttatacata taatttcctc ctataaatta ttgtttaatt tgatatttcc
    25081 tacttcaaat gctgtattta ctatctcaga atctctattt ttattttctt cttttaacat
    25141 atttagttct ttttctaata cttctaatct cttttgttca tcagtcaaaa caacttctat
    25201 attttttata tttaattctt ttgtaactgg atttatagac tctatatatt gtttactata
    25261 gtctatacta ccaaattcaa catctatgaa atttaattca gttatttctg acaactcttg
    25321 tatatctcct attgcttcac cagtttgaag ccatatcttg cctgtttggt cgtaaattat
    25381 tctattattt ctattcattt aatcacctct aatttatatt tttattcata tgctctccaa
    25441 gcaaaaccag tattaataat agaaaaaatc gtactactaa ctttatcaaa tggaacaact
    25501 ttttgtaaag aaagagtaaa actattttgt gtaactgaat ctagttttat agaagaagtg
    25561 caaatccatt gtttattata gaaacgacgt ccaagtgtaa aatcggctaa gtttgaaaca
    25621 caattaaatt gcacatcttg tgcaggtacg taactaccta aaaacatttt gtctattata
    25681 ataacacttg gaacaaaatc taaattaatg ggtataatta gtttttttac tgcggaaaga
    25741 ctatctccat caaccattct gaaattgata gtatcttctt ctctagtaaa tgtgtatcta
    25801 cctgtagccc atttttttct tcgactaagc tgattattca attctgttat tctattttgc
    25861 aaatcctgca cactagcgtc tgaactatca aaacttgttt ttattttctc tgataactcc
    25921 acaagtgtat tatttaaact tgcttctata tttttaagtg ctaaagtgtt tataatactt
    25981 gttttaccat ttctaaaccc ttctccaatc tctatcagct tagttgatat atcacttaaa
    26041 ctagcatcag attgaagtgg cattatctct ttacttatac ttaaaacctt ctcaactgtt
    26101 gcattttctg catctgtagc aactattttt aatgtgtgta ttgcattgtc tgtaaattca
    26161 tagttgatgg ttttttctgc tgttaaatct gttgttattg tttcttttaa tacatcatct
    26221 aaataccact caattttact taaattatta tctaaatcag atgctgtaaa ttttgcagaa
    26281 ttagaactat atgaattaac taaaattgtt ggcttgttat ttgttcttgt aaatgttact
    26341 gttttacttg ttactaatcc accatcttct gtacttattt ctattactat tgaattttgg
    26401 gaattgaatg ctaatggtac tatttgttca tcagttaatt taagtttata attattatca
    26461 ctagaatttg tctttgtttc tattattgtg ccatttaact tttctgtaac aatgaatttt
    26521 acacttggtt cattatctat aattctgtag tttatttcac atcctctgct aatgttatca
    26581 atcacaatat caataacagg gtcaggtata aatcttactg tagttgtttg ctgtactaca
    26641 tcatcaatat cagttgttac atttccaact ctatagctca tcatataaga ttctttatat
    26701 aaatctttat ctctgaatat atctatattc ttattagtat ataaatgaaa ataagctgta
    26761 taaagtttcc catctgcttc aaattcatac ctttgtgcaa gtatatctaa ttctgtatag
    26821 acacctcttc ttttcttcac agttctattg tatttccata tcttcacttt atcacctcac
    26881 tttctacatt ggtattaaat tactatttat tgttgatata atactgcttc tattaccttt
    26941 tacttcttgc atcacttctt ttaatgctcc ctctacgttg tcgctctcaa ataaattatc
    27001 tgtatctttt atacttgttt tatctgctgt tgtttctata ctatctacac tagtttttac
    27061 ctcatttaat gcactaacga tatttgtttt atctgttgta gtaagttgtg ttgtatctcc
    27121 tattttagtg ttcaactctg ttttagcagt ttctatgttg cttgttaatt ctgttttagt
    27181 tgtatctatt ttagtgtcta attcttgtat atcttttaaa gttgctaaaa ttacggttgg
    27241 gtctactttc aaattaatat tagctacatt agatacaact aatattactt ttattaatag
    27301 ttcttttaca gtccctgaat ccgcagtagg tttatatgtt gttgggtaac tagaaatcgc
    27361 taataattgg tctttagaat caaatagacc aacttctctt atttcaaatc ctccaacatc
    27421 accaggtatg aatttttgta ttactaccca gttaggatta tctttatctc cttgtgcatg
    27481 ttcaagtgta ctttcccaaa ccacattttt tagtgctgtc tgactctcat ttggagtata
    27541 agaactccct cctccatctc caacttttat ctttgcaaaa tctacttttt cacctgtaat
    27601 acttgcattt gctattgctg ccttaccaat gtcagttact agagtaaaat attgttgttc
    27661 tgtcaatttt atcacctcat ttctattttt taggatacaa tgttactttt tctaatgctc
    27721 tatcatttcc actacaaata gctatttctc caaaactttc taaatttcta ggtacataag
    27781 gatatattgt aactgtttct cctgtactaa ttgctgcacc tgtataaagc tcgtttttat
    27841 taaataatat cctctcgaat ttgtgttcaa ggtgtgcagg ttttatttct tctattttct
    27901 tatctaattc taaaatagtg ttataactac aattatttgt tataaaacta agtgtaaaac
    27961 taaatagatt actaaatact tctacatcaa cattggtctt tgtgtaagct tccgagatag
    28021 cttttataac ctctattgtt gttgtaccct tacctctcat ctttgctttt atattgcttc
    28081 ttctagtatc gaagtctaac ttataattta cttttatgct taaaaggttc tcccaatcat
    28141 caagtcccca agttgctgta tctataaaaa actgttctaa taaatcatct ttttcatcaa
    28201 ttagcgttaa aagttcattt tcaagtgctt cttggatttg tatgtcaatt tcattgtttg
    28261 caaaacttgg caggtaatca attaacttca atttaactca cctcgatatc aaatatagca
    28321 cttgaaacag caggaacttt ttcttcatca actattatgt ttttaacatc accatttaca
    28381 aggagatttt ttatatcatg aataccttct atgcttgcta acaaactcat tacttttata
    28441 taaacaatct ctcttgaatt ttctatcaaa taagaattta taatatctaa aaacacaact
    28501 tttatagatt caatatcata tccattttca agtgttaaag ttgcactaat attaatatca
    28561 aaagtacttg gtgtaacaat tgtaacagta ggtccaatag gtttttcttc ttctatatgt
    28621 tcagtacatc tttgcaatac ttcattgtca actgattgat tgttttgacc ataaattaga
    28681 attttaattg tacctggacc atcccaacga ggaattactt ttgcattata aacaccttca
    28741 acctctaaag cccaagattg gtaatgcgcc ttatttccac ttgtcgcttg atttttttgt
    28801 attttataga acctttcttt taattcttca tcagtttcta tttctgttcc accctttagg
    28861 tcaagttcat tataaatttt agttactcca gttatttctt ctataagttt aaattctgtg
    28921 ttagctgata aattatactt aatgccaatc tctaaagcct gtatagggct tacatttaat
    28981 ttactatttt catctattgt aatatcttta ataattacaa atagtaaatc actatgagat
    29041 attattgttc catttggtat ttgagttcca attttgccct caaatgttac ctcacctatt
    29101 gcctctgtac ctaattttct atatacgcca aattcattga ctcttctatc aagaaaatcg
    29161 tcaaaattat cttgaataaa aactctctta tgtatataag aaagttctat atataattgt
    29221 gcaagttctg aatttattgg agaaactata ttataaagag aagatccttg gcctttataa
    29281 agagggagat ttatgttatt taaagttcta ttatttaagg tactgaatga ttggccatta
    29341 tacaaaagta atctcctcct ctccataaat tgtttttaca tttaaactta tagataaatt
    29401 atcatcttca aattctgcat ttgttatatt gacttctaat atatatggat tagttaatag
    29461 agcttcttgt atataccttt ttgcctcact ttcagtaaga cctttagtat acttttgccc
    29521 tattagattt tttatatctg ttccataact ccaatcatat attaaatgaa catacttatc
    29581 tgtttttatt gttttgtaaa tccatacttt aattgcttca tttctttcaa ctattttaaa
    29641 gtctccattt tcaattatct tttcatcttt attaaaatcc caagcaaatt ctttaaaaat
    29701 aggtaattct tcattgtctg gagaaatata atcttctgga acacccatga aagggaatat
    29761 agtattattc atctagactc accaatttac ttacaacagc aaatttttca cctattttaa
    29821 acattattac tgtgtttcca gattcaaaag tatctataaa gggatttttt atttcatgtt
    29881 tatgttcttg acttgtttct gtatcaaata actctatctg tctatcaagc atccaactat
    29941 ctatcaaaat atcttctttt tctaatatga tgttatttat ctctattttt aaatctggta
    30001 atttactttt aatttttcca ataaaaaaag aaggttcatt atgaaattta ccttcttgcc
    30061 taattattcc tataaattca ttgattggat ctgccacgat atcacctctt ttacaaaact
    30121 cttctagctg tattaaaatc ttttctatta cttaatttac ttattttaac tacatctcct
    30181 gtttgtggcg catgtaaaaa ttctccatta cctacatata gtccaacatg gctgactgga
    30241 ttatggaaaa ataccaaatc tcctgcctgt aaattatttt tctctacttt cttacctacc
    30301 ttagattgct gacttgaggt tcttggtaaa ttaacattaa ccttcttaaa gcaatatacc
    30361 attagtccag aacaatcaaa gctacttggt ccattcccac cccatttgta tggctttcca
    30421 agatgttttc ttgcttctga aattactgtt tttgcttttt ctgtcatgtt tccagaataa
    30481 ccaccaatta taatctttcc ttttcttctt ccaaagttat tagcttcttc aacatttcca
    30541 aataaaatat ctatatgata tgttccatcc ttttctataa ttattgcagg cccattatca
    30601 tttactttat acgttccatc tcggcttgaa acacctgtaa ctaattgtat ttcatctcca
    30661 tatctcataa gaggatgttt gtttaagaaa tcttttgtat agtaagattt ttcatatgct
    30721 ccaaccatag gagcggcaca tgtcttttta gaaggatcta gttttttacc tctacaatct
    30781 gtatttccac cctcttcttt tctaggacaa taagccgtaa attcagcagg gtattctgtt
    30841 cctccagtat actctgaacc atcttctttt tgctcatctt ggccagcaga tttttcatcc
    30901 attatattct caaaattaag ctctagttca atttgataat ctccattttg ccatgtatgc
    30961 ttatctgtat ctatataaaa aagacctatt aattttgtat atgagtcttt tacttttaca
    31021 cctctaccag ttacacaagt tatatcacca tatcctttta aagagcaagt tttttcgatt
    31081 cctttaaact ctccatctat gtctatagta ctgttttctt gttgttgtat taccttttgc
    31141 ataattaccc caacgtcttt aaatatagag tcatttattt tctcactaat tttattacca
    31201 tactgatcaa caactagtac tttatttttt acattctcaa tgctttcaga aaaatttgta
    31261 ttgataagat taaagccttc ttcaaatgtt atatttaaag taactacacc cttctcaata
    31321 acattaaatt tatcaagatt agactctatc atatactttt ttttagttgt cttgcttgct
    31381 tctgtataaa cgctcattat agtatcgtag ccagttacac caataaacat ttttgtatat
    31441 ttaacgttag ttttaggtaa attacctagt gggaatttat tatctacaaa aacctgtttt
    31501 gcaatatctt cgactaattt atctttaaaa ttgtatgaca cttcgctttg taaaagtaaa
    31561 aaacccatat ctttagctgt aaaatctata ttattattgc tagagtcttt agatctatct
    31621 attatcattc ctctaaaaag ttctttatca tctacataga aacaaactgt acttgctact
    31681 ggaatatcta tctgcctaaa attaatatca cttgcagatt gaattataga aaattccaaa
    31741 gttcttgatg gtgatctata atcaccagac caagtgcatt tatccactaa atcagttaca
    31801 ttatatatat ttccgttttt tatatgaact tgtattttga ttttattaat tataaatcac
    31861 caccattttt aaggaattat taatacccaa ccatttttta ttacagatgg atctttaatc
    31921 tcatcttcat ttgctttata aatcttctcc cataaatctc cattgctata atatttttta
    31981 gctatcttaa aaagagtatc tccttcaaca accttatgcg ttttttgctt agtatcaaat
    32041 ccttttgtta gaggaacatt cttttcagaa gataattttt catcattatt aatgtttact
    32101 ttggatattt gtatccttct gtattctttt agacttaagg taaaataaat atctcttgaa
    32161 taatcttgtt ctctataatt aaaatctgca attatacatt caaagtttat attagtttcc
    32221 gtaatgataa atcttaatat atatccttct ttcatccaac tttctattaa atttacacaa
    32281 tcatatggtt ttggaaatcc attataatta cagaaactat attcttgatt agggaaaaaa
    32341 gaggatagtt ctatagtttt aagccctaat cctccaaata ctgcgatatc tccaacactt
    32401 aaaatattag atgtatttat tgtcgcactt ccatttatct caaaagaggg tggaataaca
    32461 ggaaacctaa atgtattatt tgcttgtctt aaccatattt ccattaaact cctcctaaaa
    32521 taaaagacac ttaagtaaat aagcgtcttt taagataatt actataaatc aaatactgta
    32581 tatttgccta cattttcata agcaatttca agtgtttttg gtgttgcatc ttttgtaagt
    32641 tcgaatgcta tttcaccttt caaagtatct cctgggcgta ttgtatcaaa cattaactct
    32701 ccatttaatt cttggttatt tattgcttct ccagtagtat tgtcacttga ttttaatata
    32761 tagttttcta gataatcact ttgtaaatca ttatcacctg tattagttat ttcaatttca
    32821 acagcataat cattactatt tgtttctaat ttcctaatat ttttaacaga tacttttgct
    32881 ccatctaaat caacaacttc gcctatttta tgatttttaa cttcctctgt gttttgctta
    32941 gatttactat ctctattttt tgaattattt gtgctgcatc caactaaact taaagatagt
    33001 ataattgata ttgttaatac tagtattttt ttattcataa cataaccccc tgtaaattat
    33061 atactacaat tatagcacga taatttacaa aacacctcca atttttgcaa gagatatttt
    33121 tttgtttatt tttcttacaa ttttatctat gtcagcctct tctcttacaa ttattgtatc
    33181 agccaattta tcaagaaata aacttccatt tgaactattc cttttgtatt gattagcttc
    33241 ttgtttggtt aaaagtttct cgccttcgtg tgctcttatt aaataatcat ttctaggaac
    33301 tctatttata ccaaatgctt ttcttggact tttgccagca gcatctgcac cttttccaat
    33361 ttgtaaacta ggaccttttt taaatatttc tattgttccc ttaattggat gtttaaagaa
    33421 atctttaagt ttattccaag cttcttttac ccctttaact ttatctttga aaagagaatc
    33481 agcaagatct attacaggtt ctagtacgcc actaactaga tcaacaaggc cattccaaat
    33541 tgattttatt acacctactc cactatcaaa tatttgttta agtccatcca tagtttgatc
    33601 agcatttcct gttataagcc ccataataac attaataatt ccagatataa aagacataat
    33661 tccatttatg attccagata ctgttgttat tattgcggtc aatgtattta aaacccatgt
    33721 aaatgctaaa attattccgc caacaacacc agctattgca attcctatag atggtaaaag
    33781 ttgttgtcct atttgtgata ggaaaggcat aaatccttga aaccaagctt taacaggagc
    33841 aagtgcttga gttaaacttt ggaaagaaag tttaattcca tcagtagact ttttaacact
    33901 atccattggt tggcttactt tgtttactgt atctccaacc tgtttggctg gagtaaaaaa
    33961 atcagtaatc gcttttttaa ccttatcaaa gcaatctttc aaattatcta aatgaggttt
    34021 taaaggttca aatgctttta ctaaattatc tatatttgtt ttaatatttc cagctaatat
    34081 gttctttaat tcattaaatt tatcttttat tgaagttatt gactccttaa acttatcctt
    34141 aaaatttact ccagaatcct ctaggacctt tagtgcaggt ttaacatcat ttaatatttt
    34201 attttgcagt tctgtaaatt tagtttttcc agattcaact ccttgactta tagagtctgt
    34261 cataggctta ataaaagttt taaaatcact aaatgaacct tttagatttg ctactgtttg
    34321 ttttaatcca cctgtatttg gtgtagattt accagctgca tctgctgctt tacttcctgg
    34381 gatatttaca tttgttttag tagatatttt ggcagttgct tcaattggat tttctaagaa
    34441 tgttcttaaa tctctccact tatctgtaag ccatttagct ttatctttaa ataagctgtc
    34501 agctatatca gctatagctt gtataggagc agttacaaaa tctatgaaac ctttccaaat
    34561 tgattttata attttagttc caccatcaaa tatttgttta actccatcca tcatcttttt
    34621 actatcgcct gtgacaatac ctataattaa atcaaatact ccatttaaaa tagatgctag
    34681 accatttaat acattagtta tagtatcaac tacagcttta aaagcagttg ccagagtaga
    34741 taaaatcaaa actaattttg ctatatgaaa acctattacg ccgattaaaa caggtcctag
    34801 taccttcatt attacacttc ctaggtttaa aatagatgca aatagtggac caaatgctga
    34861 taacaactct ttaaatttct ctcctagctg tttaagtgca ggcatacaaa aatttactat
    34921 aggctttgca aattctgtaa atgattttac taatgaatta accttatttc taaatacttc
    34981 agatttagca taagcaatgg taaatgctac tgataatgca attacagctg ttatagtcca
    35041 tcccacagga cccattacag ctaaaaatac tgcacttaat ggtttcatac caattacaaa
    35101 agctctgaaa gcaacttttg ctcttaatat tattggaaca aaaaagccaa atatacctat
    35161 agcttttgaa acaattaaag acacagcccc tatagttaat aaaagtactc caaatgctcc
    35221 aactgaaagc attacattag ttatgactct ttttattggt tcacttagat tattaaacca
    35281 aacagtcatt ttagttaaat tatcaactac agatgctatt gctggcttta gttgatagta
    35341 aatagtaatt ccagtttctt caagggctga ttttaaggtt gcaagtccac ccttagtatt
    35401 atccttcatt acatcagaca ttgtttttaa agcacctttt gaattatcta tttttttctt
    35461 taagctatca tattcatttc ccacaccatc aagtaacttt tgtaacgttt ttaattgagt
    35521 ctttccacca atagcagata agtaataatt tttctgttca tcagtcatac ctttagtttt
    35581 atttgcaacc tctttaagca catttgacat acctttaaat ttaccttgat tatcaaatgc
    35641 acttacacct aactttttca ttgccttacc tgcttgacct gcacctgttg taagatttac
    35701 catgatagag tttaaagcat gccctgcatc tgtaccctta actcctctat ttgcaagtat
    35761 tcccatgata gcagttgctt ctgaaagtgg aacttttaaa ttgctaaatg taccacctgc
    35821 gacattaaac gcttccatca actgttgtat acttgtattt gatttagtag atgcatttgc
    35881 aactttatct aaatactcac ttaaacctgc accagctagt acttttccac ttttactgac
    35941 cttatcaact tctaagcaca tggaactcat gctatcagtt actaaatctg atgctagacc
    36001 aagatccata gaggctgctt ctgataaatt aagcactggc attaaggcat gcattgactt
    36061 ttgaacatcc catccagcaa gccccatata ctgaagtgca ttagcagaat cagcagcagt
    36121 tttagttgta gcttttccag catctctagc agccttagac agagcttcaa aatctcctgg
    36181 ttttaaggaa tttccaaatg tagccttaac ttgcgacata gcgctttcaa attctgttcc
    36241 aactttagtt gcagctgcaa atgcaccacc aactccagca gtgataagac ctcctgcttt
    36301 taaagcacta gttccaacac tttttaaatt actttgaaca ttatttaatg ctcttgatgc
    36361 ttgggaagat gaatcatgta gagctctagt atttgctgct actcttttta atgttgaact
    36421 tgcttcatct ctcattttta ttattgcttg taggaccttt gtattagaac tcatatacta
    36481 aaacaacctc ccaacaccag attttatttc ttcttttctt tcatctaatt cttgatccat
    36541 aaaaacacta acaatttctt tttcgcctct tagtatatga taagaaacgg atggcattat
    36601 acctttatac ttaaataata agtacatcaa gttaacttct ccatccgttt taattagttt
    36661 tttatatctt tagctctttc tttttgtctt tttttatctc cctctgcatc tattccattt
    36721 actttattaa cctcctcgta taatttatct atttcaccac taagtaatag ctttttaatt
    36781 aattctttag gtgtaattgc actaaatttt ttaactaatt ccatattgct aaatatagaa
    36841 catgattcta atattgtatt tactttaagt tcatatatgt taatgttttc tacatctcca
    36901 ctattaacgt ccatagagtt cattcttatt tcgtcaaaac gttctggatc aatagccttg
    36961 cactctattt ctaaagtatc atctagtttt ttacaatata attcaaatgc gctagatggc
    37021 atttttaatt tacctgcatc catatttaat aatttttcta ctgtgttcat ataataaaat
    37081 cctcctattt ttcttttatt ttatctaagt attcaaagcc ttcaaatgtg aatggaactt
    37141 ctatttctcc aactttcgca gcttcccaat cagccaaagt taagtcgtca aacatacatc
    37201 cataaagtgc aattctctca gaaccatatg aatctgggtc ttgcaattta cttattatag
    37261 ttactttaaa ttctttacct tcatttaaca ttttttcgac atactcgcca aataaggtac
    37321 ttatcttata aagagttata gatccttttc cagttgcacc aatcatttta tatgtggtca
    37381 ttatttgacc acataatttt atttcttctt tatctatatc aaccttggcc tccatccctt
    37441 ttgttgatgt aagttgctta ttatctagcc aacactctcc aaaagtacca gatattattc
    37501 tttttgcatc tattttagct ttcaaatttt aacctccaaa ctacatagag atgtctaaat
    37561 atacatcttc cattgcatct attaatttaa ttcttgcttt taaaaataca agagtgtcag
    37621 tattgtattc ttttatctgt tgttcttcca tattagatat atctatattt tgttttttta
    37681 gccatgctct ttgagcatct aaattaattt ctacagttga accttcatca attaaaccat
    37741 ctttttctaa ttcttgaaga tataattgta ttgcaacaat aagtaaacac ttattatcat
    37801 aagtgttttg ggtttttcct atataatttt ttacaattat ttttttaata tcgttatgaa
    37861 tttgatctag tgtatcaacc aacttaatct ttttgaatat atcacctttt gaatcagtaa
    37921 ttgttgtaaa ggatgtaact cctctagcga ttactatagc tccactttct tttattaaaa
    37981 ttagctctcc attatttatc cttgtatttg cttcagctct tgttagctta ggtatattag
    38041 taacttctgg tatttctgca tatgttacag attgagttga tggagttcct gcaataaaac
    38101 ctgctattct tggcagaaat tcatttgaag tgtatttttt accttcaact tcaatatcag
    38161 ttgctgtaaa gtttattata ccttcataat ccgctgaatt acttgcagta atagctttaa
    38221 cctttatttt attatcttct ctcattttct taatccacgt tttgatttta ggcaaatctt
    38281 gttctgtctc atctggcata caaagataat taaactcaca agtttcaaga aaatctagtg
    38341 catcatctat tgattcatct gtatctaatg tataaactaa tactttattt ggaggatata
    38401 catttccctt catagccatt tttatataat ctaaattagc agcagaataa ttttctggaa
    38461 tatcctctat ttcttttatc tcaactaacc ctatattttt tgtatctttt aaaataagcg
    38521 ctacaatacc accagagcgt ttaacaaaag tttttcctgc ttggataaaa gaaatattaa
    38581 tttctggtaa acccatttaa tcacttccta tctctaaatc tacactttcc ataatttcaa
    38641 atgtttcatt ttcaaaatag acttcttcaa aataagatac agtcattaga aaagttagtt
    38701 tatatcctac aggatctttt gcgattgatg gagatatatt ctcaatgatt aaacttctat
    38761 ctttaactct aatgtttctg ttaaatattt tttctaatct actttgtatg tcgtatagat
    38821 ttaatttatt ttttctagcc ttttggtaat attcaatatc tatgaatatc tttttagtat
    38881 ctaaaacttt atttgcaata ttattagaaa caggtaaaag ttgcacaaaa aaacaagcct
    38941 cctcaaagcc ttgtatatta tcctcattaa taaaaatatc tatatatcta aactcatttt
    39001 cgattctttt attaactgct attataatgt ctctgtttgt aagcaactaa tcacctacca
    39061 atcaaataaa tttgcttttg cacttgtcat attttctttt gttctttcaa ttcccttttc
    39121 aaccataaaa gaaccaggga caatgccacc atttttagtt gaatggccat tatttactaa
    39181 agtagcataa tcaacttcat ttttaacaat ataagtatca tattctggtt gcttttcttc
    39241 ccatcccttt tttaactttt cagtttttac aggagtttca tctcttatat tttctgctag
    39301 ttcttgccca gtttcttttg taaacttctt gatttttcta ttaaagtttg atgcatttag
    39361 acctaaatct aaggctaatg cttcaaagac tcctaaatta tctattccca ttatatcctc
    39421 tccaattctg taataggtat ttccatatgg cttggatata tatttgcctt agaaacctta
    39481 aatacagctt cttctccatg attataggtt gtatttatag tatcacctat ttttatatct
    39541 acttctggtc tacaaaataa ctttctattt acaactatag atccaatatc accatttaca
    39601 atggcttctt cttttctaga aattgcacat gggatatttt cagctataac aacattacta
    39661 aaatcattag aacatgtttc ttcattccag ccttttaatt ttctaattat agtcatttta
    39721 tcaaggtatg tcatttctaa tatatctgct tcagtcattt aatcacccct tagtacatac
    39781 taacaataca aaatgggctt agaaattttt tatcagtatc agttaaaaaa gatgatgaac
    39841 tgatgctatt ttcagcagtt tgtaatgtta aagcattata ttctatctta gtgtcacctc
    39901 tagttatagc ttttaagctt ttttctagat tatcaagtcc actatttttc ttaagttctt
    39961 caaatcttga agataatatt gatattactt tttcttctac aaacccttct agtgctgtac
    40021 ttagttcctg cctagaacaa tattctaaaa ccatctcact tattttattt atataaagga
    40081 ttattaattt gtcatatcta tcattttcta ttcctaaaac taatttaaca ttatcaagca
    40141 ttgtactcat ctagtagttg aattaattcc tctttagttt tagttgaata tccctttata
    40201 ttcttgcctt tagcaatttc cataagctct ggtttagata gattaaataa atcattagag
    40261 gttgatttaa ctttttcttc ttttttctca atattctctt tagtcacttc ttctgaatat
    40321 attttaaatc cttttgaaat caatttatct ctcgaatttt catcttcaac aactttaatt
    40381 atgttatttt ttatcaatgc aaacatttaa aacaactcct tattcttgtg cagctttagc
    40441 ttgtctcaca gatgctctac aaagttttaa atattcatcc aatatccaca aatcgtgata
    40501 ttttctatag tctatttgat aagcatctgc tgtttggttt tgatctggag taaatattct
    40561 tggcttatca gtcttagata ctgcaattgg agcagatgct ggagttatta accagtttat
    40621 atctaaagcc tttgctcctt cagtaaaccc accctctgtt tgtccagatg ttttaccatc
    40681 tctaaatacg tattctgttt tcattctttt tgatggaact tttattatag gtacattgtc
    40741 tatagacttt acaattcttt ttatatctcc atcgccaaat tcaatatcat caagttgaga
    40801 tgattgttct aatatgcttg caattatgct agacatacta ataataagag gtatatctcc
    40861 agataaatct tgtattgatg caagttgatt tttcaattca cttaatattg tagttactgt
    40921 agcttcgtat gaatatttta cacgctcatc tactttagct gtttcagaaa atatctttga
    40981 gtatctatat gcatctattt ctggtataac atgttgcttt tgaaataatc ccattacaga
    41041 agatgcattt ataacgaagt tactttcatt tacatccatt gaatctaaca taaaagtcct
    41101 acctctatcc atctccattt tcattgtttc gtactcaagt gtaactgcgc cttgcgtata
    41161 tcctttagat ctgtcataat cacctaagcc atccattgtt acttttggta cttttacctc
    41221 tcttcctcca ttatatataa cttgaccagc atttttctcc atccatccag tagttgagcc
    41281 aactgttacc tgcatatcta gttcttgttg aaaaagtgtt acatattgta atacattagc
    41341 catttataaa atcatccttt ctttttttat actaaagact atttagccct tgctttattt
    41401 gttttctaat ttctgatagc gaacctgcac tatctttgcc aggtggaacg tatgaaccat
    41461 ctttaattct ttcatctacc tttgactgca catattgctt cattgaattt tcaaatatct
    41521 ctatatttgc atttgtttta tcatcatcat cagataatag atattcaatt aagtttgtag
    41581 gtattttctt ttcaactaaa atatctttat atttagaaac tttttctgcg tactgctttt
    41641 ctttttttag tttgtctagg ctttctctta attcccttat tgctttttgc tcttctgttt
    41701 catttgctcc agttcttttt agaacttcag cacttataag tgtctgcata tcttttttct
    41761 taaagttttc aagtgcttcg ctgtgatatt tatttttttc attatctata atagctttaa
    41821 agtttgcatc attattaatt aattctctaa actcttctat tgtaagcttt ttatcagatg
    41881 cagatttgaa taaatctgtt gattgcagca atgaatctat ttctgcatca tcttctattt
    41941 cttttagtaa ttccaacact tcttttttta acatgtataa agttctcctt tttgaaaatt
    42001 taattcaaac attctatttt atccataacc tttactattt taggaaattg aattgcaaag
    42061 aagtcaatca tttcttcgtt tgtagcccaa gaactacaac tcttcaatcc actttcacta
    42121 agaaatgcat gaattatttc atgcctagca acttcatttt tatatacact taaatctttc
    42181 aatgaacctt cttcagaatt tattttctct ataacaattt ccttaacaga gtgatcacaa
    42241 tatccagctc tttcctttaa tagcacatct tcgctttcaa gtttctctat cactagatat
    42301 tctgttccta atatttcaac tttcatattt cacctcatat tttttagaca taataaaaac
    42361 acctattaaa ttaattaata catgttaaac aggtatatat tgacatctac agtgacagtg
    42421 tcttggcaaa gagggcctat cttcattgac ataaaatatc actccattat gcatagaaca
    42481 atcactacat gtgcgagaat ctaaatctgc acaatacatt acttttataa tcccttgaac
    42541 cttgaaaaaa tactcattta cttcactttg acatctggca acttcatttt ctattagtct
    42601 gctggtttgt cctgcacctg ttgaatattt atctcttatt gctttttcta tcttatttac
    42661 agaagttttt ccatttagaa aactctttat ttcttttttt aaggactctt ctataccttt
    42721 tttattcttc cagagtctat cgctccaatg ttttccatca accttattat tcactatctt
    42781 atttataact ttattgttta tattactaac atcttttatc ttcagtatta ctcctaataa
    42841 aattatcaaa tttgaatatt tattcttaga tgaatcagat aaaatagttt ctatatcctt
    42901 gacttcttca tcataactat tagaatactc atttataatt ttattaagtt ttgaccttag
    42961 atttttctta tctttattat taatatttaa gtattcatta ataatatcgt gtgttagcaa
    43021 tatctttgct atttctaata aaagtttatc cctattttct ctttgatact tataaaatgg
    43081 ttttgtccta ttttcagcag atttatatat atcttcagtc aatttagtta taatcttttg
    43141 attcatctaa taaaatctct ccctctgaaa ctatttcctg ttctttttta gctttttcta
    43201 tttcagcatg aggattatca atgaaactta gttgagataa gccagtttca gtagataatt
    43261 tatctccaag ttggcttaat atatttgcta caactaaatc atcacgtggt atcttagggg
    43321 taaatttagc cttaatatct ttataatcat agtcaaaacc atatttttta ttaagccaaa
    43381 tgcacaataa tttgtaccta ttaaataaag tatctgcaat actatcatta ttttggctac
    43441 aaatccattc tgtactgatt aatcttgcta taactgctag acttgatgta ttactttgca
    43501 ttttttcatt gtgatttata tgagatgtaa tttcatacat attttctttt attgaagata
    43561 gtgtattttg aacaaatgta tcattcatat ctttaataag ccactctatt tttccatctc
    43621 cattaacttg aagtattcca agttctttca tccttggcaa atcatcttct ttaatattac
    43681 atccagaaaa ggttaaataa gcatttctaa aatcactaat ttcattactt atatcactta
    43741 aattagtttc ataagcatct tgtaaccctt ttatatcatt aaatattgta ttttttccta
    43801 gttcttcact tcttttgcaa attgatattg gaacatttcc atcaaatata ttgtttactg
    43861 taggactttc aacttctttg aaatttgaat caaaatgata tatatattct ttgtcatata
    43921 catctacata cgtgtcatct tcgaaacctt tggtaaactc tctaataaag catactattt
    43981 ctccattttc attttcataa tgacagccat ctgttggctt gattatcttt acttgtattt
    44041 catcatcttt tacatagtaa agttcatagc aaagcccaaa taatagtgca tacctcaata
    44101 aatctgagtc atgtttttta ctccaatggc aagtatagta ttctaaatca tttataatct
    44161 tctcatcacc agatctactt gtataggtaa tcttatttgc aagagaataa gcgacttctt
    44221 cattaataaa cttttttaag aaattagtgt ttatcttatt attagatctc ttggtaacca
    44281 ttttatagtt gctaatagca tccgtttcac ctttataata cttatacata gtatcatatt
    44341 cggatttatt agagttccaa tcgtcatata tgtcttttat taagtctaaa tctaattcca
    44401 ttaacaacac atcctatcct gtagactcta taaattttaa aacttttatt ggaatatctt
    44461 gctttgaaat ataactttta ctccttgctc tatgagtata tgcaaaatat cctttttcat
    44521 cttttcctat tccaacattg aacttctttc cttttttagt taaagttttt tgaacttctt
    44581 caaactttga tctattagcc aataaattac ctcccaacta aaaacctaat aattttctat
    44641 caaagatatt tactgtctca gaaactttta tgttgttaat tcgattaata aattccgatg
    44701 atatatccgg agcatcatca tgtagagagt atttttgtcc tgtaaaatca agtatttgct
    44761 gtataaattc cttatcttct tctgcaaaaa tcatttggcc tttgcttaaa acaggtatta
    44821 aagtagagat tttatcatct ttatttttct tttgttgctc attaatgatt tctatatctc
    44881 tataactaag aacattatca ttttttattt taaactctaa ttggttggca tctgcgccat
    44941 taaatgtatt tttttctata taaatatgtg ttatatctga atacactttt aataaataaa
    45001 tcatatggtc tatatattta tcaaaatcag ttctagcatt tattttagct agttctgcca
    45061 atcttccata tagtaaacca ttttctgatt cacttccaac aagaaaagcg ctatagtcat
    45121 gtttcgaccc acccgcagat gctggatcac ataacagcat tgtcttttta aaagtatgcg
    45181 attcaatttc ttctctagtt tctgttctaa cagttttaaa ccatttttct cctatagaat
    45241 tgacatcatt ttgcacttcc tgcttaaaag atgttggatt ttcataataa cttagagcca
    45301 tatctaaaca atcccaaaaa cttggccata atatagggaa tttcatttct ttttcatttt
    45361 cccagtaaaa ttctttagca tcttcaagat gatttgtatt tttaaaatca aataagatct
    45421 ctttgaactt aagccataaa ccagaattaa ataattcatc tacattatca actaaaactc
    45481 ctttttcgca tttaaacttc caagttggta aattttttag tctactataa aaacattctt
    45541 tgtgttgtaa ggttcctaat gctataaaag ttgttcctct ttttatagtt ttaccatttc
    45601 ttatgactgg tttttgagca gcatatttta catcatcaga aaatctttta aattttttct
    45661 ctctagcatc ttctgttcta acattttctt ctgactgata atcatcaagt atgataagat
    45721 ctggacggtt attattgtat tttcttcctc tcatgggtga tgaagatgat atagcttcaa
    45781 taaaagtttt atttgttagc tctagttgag tactattaca aatgtatttt ctatcattat
    45841 catttaaaag gacaccaaat gcttttttaa tatactcatt ttcaagtaat gcatttttta
    45901 tatcttttac aaatttctct gctgtagatc ctatgtcaga gcaaatcaaa gtatatgtct
    45961 tatgtttata gcaatgcgac catacagttg tcgctaaatc tccaaatgca cttttacctg
    46021 tccctcttgg ataaattctt cctaattgct gtgaaccatc tccaattata gattcttgta
    46081 tatcttccca tagctcatga tgaactttag ctataggagc agcagcatta tcttcttttg
    46141 gtaaatatgt gtcttgcata aaatacatac agaaaaattc taggtttatt tttccaagtt
    46201 gataagcgag accattaact ccccaaagat tgtttgaatt ggcttttata atctcatttg
    46261 cttctttttc agcatctttt atagatgctc catttcttat aaaagtcttt gctagataac
    46321 taaatattaa ttgtatattt ctttctagat tatccactcg tatttacttc ctcttacttc
    46381 ttctattttt ttaggctcag tgagtaatgc aattaataaa ataaacaatg taaatttttc
    46441 tctaataaaa tcaccctctt tgaaatacat ctctggtttt taaattttgc tagaaaattt
    46501 ctagaactca ttttccataa aaaataattt tttcaaaata gaaggacacc ccctattaat
    46561 aaaaaaaata aatatattaa agtattttga attattttct tgcaagaaat atttttttga
    46621 ttagaattat taattatctt tcaactattc cttaaatatg tattttcggc atagttaaaa
    46681 agaacctagc atttccaata ggttcacata tcctataata atccacatca cttcactgta
    46741 tgaaacttta attattaagt ctcactaaat tttattaaat tagctataac taaagttaaa
    46801 tacaatatat gatttaatta ttaaagtaaa tatatatata atatatgttt tgctttaatt
    46861 gatttcaaac ataattaaaa catattcttg caggatttat ttcatatcta attacttctt
    46921 gcttgttctt attcttatct tagctatagc tgcttctatc tctgttgcat ccatacccat
    46981 gccaacacta ttatcctctg cttgtataac tgtacttgta ggagttccat atatacgatt
    47041 tataagatac tgattggctg ccaacataac cctcttatcg ctcttgtcac tagctaattc
    47101 cttgatattg ttgatataac tatctaaatc ctttaatatc attaaatttc cttggtttgt
    47161 tagctcctgt ttccgtctgt ccatttcttt cctaacatgc tctttattca tccaattata
    47221 gattgttttt ctatttactc caataattct ggcagtttct gttactgtag cacctgtaat
    47281 caatagagtt accatattgc actgatcttc tgtcaactct ttaccttttt catagctact
    47341 atcgattatt tcaacttcat tcacatccaa ttaatcacct ctttactcag cttctaaata
    47401 aacatttaaa tatctacatc cctataaatc ccttgaataa atgaccttta atatatattt
    47461 tcagacattt taataaatct attctattcc ttgtaaactc tattgttaag gctctttgca
    47521 ttctaataat gtcctgttgt tgaaaaacgt aacatattaa attttagata aaatacaaga
    47581 tattttaatt tctttttctt ttctttaact tattagtaaa tttagttatt gcttttactt
    47641 cttcatcctt atctattcct atatatcttc tagtcatttc tattgtctta tgtcctagaa
    47701 tcttccttat aagttctaat gcataagtct tattctcttc ttcatcataa atatgccttg
    47761 caaatgtttt tcttaaagag tgtgttgcaa cattttcttt tattcctacc ttttttgctg
    47821 ctccttttat tattcttcct gcttgtcttg tttggatagg tctattaaat ttcttcctct
    47881 tgccactttt aaataaaaat tcttctcctg gtttatcttg agtataattt tctatagcac
    47941 tttttattac atcagataaa aaaacctctc tattatagtc tcttttttct tcgataactc
    48001 taatccaatc cttagatact cttattttat ttcctttttt atcgagtttt aagtctgtta
    48061 catctgcaac tttaagtgct aatatatccc ctaccctaag tccagtacac attccaaaga
    48121 gggctaataa atagtttctt tcattccatt cttctagaaa atttaaaagt ctaaatacat
    48181 cctctatatc ttttatggga ttaacctttt gtccactatg tgcttccatt cattaaaaac
    48241 actccttatt tccataataa ttttttcttt gcctctcttg aactaatatt tttaagtcct
    48301 caactacatc agtaaaagga tctgtgatta tagaggttat atcatttgta ctatattttc
    48361 tttctcttgc ataagtgtta gtctttattc taaatcctaa gtcgtttaca gtttcagtta
    48421 cttttacaac attttctaat tgcaaatata atttaaatac aaccaactca acgtgatcag
    48481 actcatcaat tatacttttc tctaaaattt ttagatatga gtctatctta ttaatattag
    48541 aatcataatt tttcttaacc ttctttttta gtttcaagag tttttcttgc tttttcttta
    48601 taaaatctaa agaacattct cttccataca taccaggagt ttctttcatt acattgtcgt
    48661 aatactttag attgtctggt acatatgcag cacttgaaat ttcttctgtt atatcacgtt
    48721 taatcttcaa cacctctcta gacaataaaa aagacttaga acgtaaattc caagccattt
    48781 ttattaaata tatttttctc acaatacaat cttatcatga ttaaaagaca caaaacagac
    48841 ataaattggt cacaaaccgg acatttttta gacacaaaac agacataaat cggtttaaaa
    48901 tcgtcacaaa tcggacattt ttatagcaat ccttttatct tatctataat atcaaacctc
    48961 atttgcttac acttactcgc tgaaaaacca atcattctgc caacatattc ccaagatgtc
    49021 ctgctacttg aaaagtattt atactgtact atcttttttt cttcttcact taatattttc
    49081 attgcattat ctattttttc tattatcaac tctttatgtc tcttctcttt ttcttttaag
    49141 gcaattttct tttctttttc ttcagtctct atttcaatta cacttttaat atcatatgtc
    49201 ttgcctgtct tttctttaat ttcaactcca ctacatcctc tgtagtcgtt ttttatcatc
    49261 tgcatatcaa tgtctatttt ttctatttcc gcttttattt ttttataatc atataactta
    49321 ccttctgcac tactaaacaa attgtctttt ttactagatt gcattacctc acactcctaa
    49381 ttatgttata atgatgttgt gatgacggtt ttagaatttt gacaagcagg agcgtgaagt
    49441 aatgctcctt tttttctttt ttgaaatata tcttattctc taggcactaa ttgcaacttg
    49501 taatcatcag aactatataa tttataacct cttaccttca agtctaattc tagttgactt
    49561 aaacttttat aagcaccaaa tgaaccatat ccattctgca tatttataat aacataccca
    49621 cctattgttt cctttaaaga aagaatcata tataacttat taccaccatt aatttcaaca
    49681 atatctccta cttttaattc tctattattt tcagttactt ctatcaacat attatattat
    49741 ctccttactt taattaatat tttttagaaa ctctcccatt ttcatttcat caatgctttc
    49801 attaactcct tttatagtac ttaagttaac taaaggcatg cacaaattcc atagtttatc
    49861 tacatctaaa aattgtattt ttgcattaaa ttctttttca tcaaggcatt ttaatttctt
    49921 gttcatatta atcaattcat taatcattga atttttaatt aaattaagta tatcgtctcc
    49981 tatttctttg agatcccatc ctcttccatt tagtttctta ttatgtgtct ctactccttc
    50041 agtaatatag ttttctattt ctaaaacttg aaacattgtt aaaatagttt gacaaacatc
    50101 taatccttct gcaattatat ctttagttat tcctgtatct atagcatttt taaactcagt
    50161 tacctcttca tcaatttttg ccaactgatc ttctaccttc caattaggta tactgctaat
    50221 taactttaaa tccatcttta gtatctcccc tcattcattt attgaagtcc caactctttt
    50281 tgagtgtatt tattcataaa tatcatttca tcacaactac taaatataaa taaatctcca
    50341 tctaatataa attgcaatga tgcaatatct tctatatcag ttaaattcat ttctccattt
    50401 aagacatttt cattatctat gtcatttaat tcaaagaata tattttccgt ttcaaatcta
    50461 tcaattaact catttgcatc ttttatattt ttaatagcat ggtacacctt acatttcata
    50521 cctgatcttc tcctcttaca tctttattta tcttcaagcc aatcattttt taaataataa
    50581 aatacatata taatgattgt agttattaat atccagatga tccaaaacac tactttaaaa
    50641 aaccctgtat taaaattatt attttctaaa acattctgta tagactcacc cttaaaaact
    50701 tttatgtttt tatcagctgg attcgctata gtattatttt ttaaattagc atacatagta
    50761 ccattaaatt ctttcggtat aacttcataa tagtaccttc tcttattatt aatgtaagca
    50821 taattacctt ttattttaag attattattt ttaaatatat ctttctttaa tgccaatctc
    50881 tctttagata aattagtaat tactttcgca tcaaactctt tacccatgaa attaaattta
    50941 ttacattttt tctctattga atcatggtaa tcccaagtcc aatatgtttc agtatattta
    51001 tgtgaatggc ctttactgtc tttagtggta accactctag tatgcatagt gtatttttct
    51061 tccgttcttt ctataaacca atagtcccct tttaattctt ctatagatac agaatctaca
    51121 cttttaaatc ttccacttat caaagaatct cccgcacttg tttccatagc ataatcaaag
    51181 agatctttgt cattatttat tttagttgct ttgtaatatc tttcattatc ttctaaaaat
    51241 tttgattgaa ctttatcatc aatgaaaaat ccaagcacaa tcatcatgca agcaattatt
    51301 atgctcacta aaaattctct cttagttatt tcaaaagttt taaatttcat atgtgcacct
    51361 cttaatcaaa taaattttga ggtgcatcct cttttgcttt atattcagta tatttagatt
    51421 ctattttttc atatcccata ctatttagaa ttaaatttgc tggaaatttt cttatatatt
    51481 tattgtaatt gcgaacttgc atattataat tttctctatg ttgtgcaatc atattttctg
    51541 ttatactcaa ctcactcatt agttgcttat aattttcatt agcttttaaa tctgggtact
    51601 tctctgctac tgcttgtata tttaattttg cttccttgat agattcatca tttgcactta
    51661 ctctcatgtc agttacttct tttaatgttc tattttcata tttagcatag tcttttacac
    51721 agtctactat gttcttaatt aaatcaattc tccttttttc ttgcacctct acagatgcag
    51781 ttgctgattg aacctgctct tctaaatcaa ttgctttatt tttagttact aagaacaagc
    51841 aagcaaacat aattaatatt gaaataacta ctcctacgat tattaacgtc tttttcatta
    51901 tttcatcgcc tcactttcaa gccatttttt tctaccttct aaacatccgc ctaagcattc
    51961 ttgaaaatca taagcacaac tagtacatcc acttccgcca tttgcaagaa attcagccat
    52021 ttctattttg ctcatatttt ttatcttttc ataattagtc atcttcttat tcaagttact
    52081 tctaaataat ctgtcataac atttgtcaca tacatctttt ccgatactca ttatattacc
    52141 ttcatcatca attctgaata cctttatgtt tagaatttct gctattgttt caccacaaaa
    52201 atcacacttt ataacacaag ccatacttaa tgcctccttt tgttttcttt acaattcata
    52261 tcttgataca tacatccaca atccttacat tgccatacta ttccaattga tgtttcaatc
    52321 ttaaatacac taccattaca aagtgggcac tgattttccc taccaccaat tattttagtc
    52381 ttcattttgc ctccaatgaa cttaaattat tatattattt tcctagtata ttaatttcag
    52441 attttaattt tccaagcttc ctgcatattg tcatttggtt agtcctaagt attttgccta
    52501 tttctttttg agtgtatcct ttttccctta agtatgttat tttctgtata tcttctattt
    52561 tacttctttt tatacaagat ctaataacat gaacattttc tatatctaca ttttcattac
    52621 cagccaatct atcttctatt cttatttctt caccatgagt atttttatag atgcattttt
    52681 caaaactatc tatatctgct ctgtaatgtt ttttaacata cctacttata aatttaaata
    52741 tttgaccttt tatacacaca gctgcaaaag tggtaaattc aatacctttt ttactatcaa
    52801 aataatctgc tgctttacat aatccaatac atccttctga aaatgtatca tcttgaagat
    52861 atggatattt tgaaataaat actttgaaat atcttcttat aataaaccat acaaaattta
    52921 tattttcttc aactaattta tttctctcct ctaaacttct cacataatca ccatccaatt
    52981 taaaatggaa tatcttcatc atctaaaact tgaaaactaa tttctttttc ttcatccttt
    53041 ggcttgcgat caagcgcatt aacaattttt gttcttactt ttgtgaaagt ttttttctct
    53101 ccatcttttt catatttttc tatccttatt tggccttgaa ttgaaaccat tcttcctttt
    53161 gcgagataat tagcacaaaa ttctgcggat cctcccataa cttcaattgg tataaaatcg
    53221 acttctctag ttccatcctt ttttgtataa cccctatcaa ctgccaatgc aaaagtagca
    53281 actggagtac catactctgg tatatatctt aattctggat ctcttgctaa tcttccaact
    53341 aaaactacat tattcatatt gcaatatctc cttcattgta tttattttct ctatattcca
    53401 tttgttttac aatctcaact gcttcattaa aggcacagcc tctttcaatt tctttatttg
    53461 cagacatgat tgcattagct attttatctg taaattctgc tctttctaaa atgttcatat
    53521 gagaaatcat tttcatctaa tcccctcctt ttttactaag aggacttatt gtaagccctc
    53581 ttagattttc aattaagcaa ttatatttac atttttaaaa tcttttaatt cttcttgcaa
    53641 gtacgcttta atgtttaaca ttgcttgatt tttccatgct ccaccatctg cttcatataa
    53701 tcctgctgct ggtccagaac tcattctgaa aataaactca ctttctggtt gctctatctc
    53761 ttggaaagtg cgataaggaa ttaactttac tctgtttggg actattgctt ctgatacact
    53821 agctacgcct gtttttattg ttacagactg agaaactcca tcatctccca cattttttac
    53881 tgtagattct tggatatttc ctgcaatttt taaaagtgta tctctatcat tattctttat
    53941 aaatgctgat tgtagcatta tgttaaaact atctaagtct aaaggtctgt caaatctttt
    54001 ctctggtagt agtgcaacac attcaatata tgtctctcta tctctatttt ttctcaaagg
    54061 agattttaaa agcacttttc ttggagttag aacttgtatt aataacaact catctgcctt
    54121 atgatctatg ccacttttta tataatctac taaaccactc aatgtagtta tttctaattc
    54181 attagctttt ggttctttta ttatttctaa tgcatctgtt gtgtacttaa acccatctac
    54241 acatactgtt tctctttttc ctaacttaac taaatattcc attgcatttc ttatcatttt
    54301 ttttattctc cttttttatt taaatttata ttttacttac tttcttaaac tactttgaac
    54361 ttgcatttct gaaatcaatt acaccagcac tattttcttc tgtttcatta gtttcttcac
    54421 tatctggaat atccatgacc aactgccctg ctacttgatt tttaaattca gacgcttcaa
    54481 ctcttcctgt ctctaaatct tttcctatca gaactttagt tgttgtagcc tttgcttcta
    54541 ctatagttgc tttggatttt atatctactt cagcaagatc tctatcttca tttgctttaa
    54601 acactatctc taaactaatt tttcttgttt ttttaaaatc tgtatttgga tctgcaatgt
    54661 tatctaaaac tttttgtaac tctgtattaa acttttcttt tagaactcca cctgcaaatt
    54721 tttctaatgc tatcatgtaa aatcactcct ttatttattt aaaataatat ttttctacga
    54781 ggaaattaat taataaactt tagctctaat ttaataacac ttttaaatct ttttataaat
    54841 ttatatagcc tttttatcct tacactactt ttaagttttt ttgctagcat atctaaacct
    54901 gtgtgtacat tgtcaggaaa ttaatttata taaaccttgt aaatgctata ttttcaaact
    54961 ttttttagta ttgaaattca atactttttc aaaatccgtt tttcaatatt gaaaaatgat
    55021 ttttttcaat attgaatttt taaattattc aatattggtt tttcttataa aatcattact
    55081 taataaaatt tcctattttt tatacttttt ttaaaataca tcttctggaa tttctacttg
    55141 aacatattgt ttatttacat catatttgta atctctgttt aactcctcac tagtcgtgta
    55201 aagtcttttt cttctttctg aaaaattcat tttaaaactt atattctgtt ttcctgttat
    55261 tctatttttt aaaattaata aataagtgtc gtctgtctct tcttcttcat caacaactct
    55321 gtaagttgat aaaatataat ctgcataatt aacaatctca cttgctccag acacatcata
    55381 catagttggc ttcatgtcac ccatattttt acgaggatgt gcaactaaaa ctattactaa
    55441 gttatatttt ttagcgagat tttttaagtt gcttactatc tgctcttgtc tctcatattt
    55501 atctgcctta ttgccagttt ttattgtcat tagattgtca agaacaaaga acctaacccc
    55561 tttttttaaa tataaatgct ctatggtatt tagtagattt acttcatcac ttattgcatc
    55621 ttcatcatag agaaaaaatt tatcttctat ccagtctgat ataagctcaa cggcgtaatc
    55681 tggaatatct gtgtatgttc ctccatagac acttttatat tcttttatgt gataatcatt
    55741 agctacagtt tttctaaacc aatccataga catgaaagag ggtaattctc cagaatacaa
    55801 aaatgctttc tctccattcg taattgcttg tgctataaac tggttaacta tagttgattt
    55861 accagatgca ggtttaccag ttaaaattgt aagagttcca tatctaaatc catctagcat
    55921 attgtttagt ttattaaaac ccgtgtctat ggacttgtgt acgccttttt cacgttttat
    55981 ttttgatgcg tcaatgtaat tatcatttac actactgctc gtcgtcacaa gtgcttttaa
    56041 atcgtttata tctataatca attcatctat acttgtatct gaatttgaat tattaattaa
    56101 ttcatttgct aactctgaaa tttgtctttt aagtaacttc tcttttatga tttttatata
    56161 gtgatctatg tttgaagtag ttataactat agttgttaaa ctggtaatgt aactaacgcc
    56221 tccaatatct actaatctat ctattttttc taattcattt tttaaaagca aaagatctag
    56281 tggtttattt gagtcaatta ttttcttcat gcaagataaa ataattttat gtgactcaaa
    56341 gtagaaatca ttttctttta attcccttat tttgtaacct atagagtcat ttataagaaa
    56401 acttcctaga atgttttgct ctgcttctaa agagtaattc atttaatcac ccccagctta
    56461 ttattaaaat gtcacaacta aaggaatcac cctttcgacg tcatacaata ccccacaaat
    56521 acgatctctt ggaacatttt tgtaaactaa aatatcaatt atgtcggcaa taagttttgc
    56581 atagtatttt ggagtttcaa atccataact tctataagca agtttttctt caacaaattg
    56641 ttcgcattta cttcttgtac ttgttctagt ttcacaacct ttacttttaa atatcatttc
    56701 tcttattttt ggatcagttc ttaatccttc aataagtctt tgtatatctt gatctgtaaa
    56761 ttcatgtttt tttctttcca cttagcatac ctcctgtctt taactattta gttgcttctt
    56821 ttgatttgaa actattctac accatgataa tttacatgct ataattaatt taaacatact
    56881 tccaatatgt ttaaattaat tagaaaggtg gtgagagtat gagccaatat cgagttataa
    56941 gcaaagaatg tcctgtaata aacgaaacca tttctcttac ttgcgaatat tcagctcctc
    57001 caggtacttt tggatctaag ttaatgtcaa ctagatgtga tggaagtcat atttgcgact
    57061 cttcttctaa tgaagaatgt cctattctaa aaaaacttct tagatagtag ctttgggatt
    57121 ttcatctgga aaacaatact taaatgcagt cacttttaca cctgttattt tgcttaattt
    57181 agtaaaagaa tgatacatac aggtaaaatc tccctcacat tcttgcaggt aatcgcaaac
    57241 ttcacatgat gtgggggctt ctatattccc tctaaacacc atctccataa cttcattttg
    57301 caaatgtctt gcaatacaat gcaattcttt ttcagtaagt tctaactttt cctctttcat
    57361 gaacaacccc ctaatttatt taacatttaa ttatttatct gtttcattga ttttataact
    57421 aaatttaatt gacctatgct ttaattctac atcatgataa tttgcatgtt ataattaatc
    57481 ggagcatatt gacagtatgt tcaaattaat tatagggtgg tgatgaatat gtgcaaaatt
    57541 aaaaccttaa gagaaaaatg ctctcttatt gacgataaag ttgaattttc tggtgaatat
    57601 aaaacttttc ctggattcac tggatcaaaa cttttagaat gcgaatgtaa taaaaattac
    57661 atttgcaaaa ttcaaataat gactgctaat agcaactgtc ctctttttaa aaaagcacgt
    57721 caattacaat actaaaattt taatttacta aatttattta gtaaaaatat ctacacctgt
    57781 catttcactt aattttataa aagtgtctat atgggtgaaa tagtccttgc attcttgcaa
    57841 gtaatcgcaa acttcgcatg atgtaggggc ttctatattc cctctaaaca ccatctccat
    57901 aacttcattt tgtaaatgtc ttgcaataca gtgtaaatct ttttcagtaa gttctaactt
    57961 ttcatctttc ataggcaacc tcctaattta tacttaacta aatatctgac ttaatatcga
    58021 actcaatatt aaattgggca agtttattaa tcgattcttg gagttgacaa attcgctcat
    58081 tgacattttc tatcaattcc tcaaattcct taatatccct aacgcctaag aataacttca
    58141 cattagaact attcaaataa acagtctctt ctttttcatt catcctatct acctccttac
    58201 tatttagttt ttaaagttct ttaaatccac taagaggatt cttagtcttt gtaacttctg
    58261 gtactatacc tttactagca tctccattcc cccaagtcag caaagcccta tagtggctct
    58321 tatacctact ccctttacca tttatgatat agttttctaa actaacaatt ttatcgtgta
    58381 tgtatttctt cccaaacttg cttataagtt tgtcatattc atctttagtt agttttactt
    58441 tttctatatc taagtccaag aatgataggt ctaaataatc agttttagat tctttttgtg
    58501 tagtggtatt actttttata taagtctttt tattataagt ctttttacta ggcacctcat
    58561 gcctaacatg taaggcacct ggtgccatag ggttaggcac ctcatgccta acgttaggca
    58621 cttcattctg tacctttgct tttgaagata ttaagaaata taagttactg tttttctctt
    58681 ttttgccttc gtggttacga ttttctctta ttattagttc cttattctct aattcattca
    58741 aaatattaga tacttgcctt atactgcaac ctgctttttc tgatattgtt ttataacttg
    58801 ggaaacaaga actttcttcg ttactatatc tagctaagac aatgtaaatc attttttcat
    58861 agatgctcaa atcttctcta tcaatcaaat cattttccag ccaaaaccac tctttttttc
    58921 ttgcatcctt caaaagatcc aaagttatca cctcccttct gaaattactt taagataata
    58981 acttaagttt atagtcatca agtattagat cttctttagt caaagtttta tatgctattt
    59041 cctctgtttc tttttcataa tacatgacta gtatttcatt cttttctact ttcgtaatga
    59101 tgcaatcagc ttttgtatca aaccttatat cttgtaattg aactgctgat cctatttgat
    59161 ttttattaaa atctgagatt ttcaatcttt tcaccccttt aattttgtat attattttca
    59221 tcttcaatgt cttgtatttc ttttaatata tctactttag tcaatgctaa ttttagtttt
    59281 gcagggccaa gtaactccaa ttttctatat atttcttctc taattttatt tatatcagtc
    59341 attttcaatc acctcgcttt tgttgattgt gtaatacaat tatattacat gtctgcttac
    59401 aagtcaacta ttttgtaata cctttttctt ttatttttgt tgacttgtaa gcacttttaa
    59461 aatataatat ttcatatgga gggataataa tgatagatga aatgaaacca gaaaacaaaa
    59521 gaataagaga actaagatta aatctcaatt taagccaaga agagtttgct aataaactaa
    59581 atttaaaaag aaacacaata tctttaattg aaaatggtaa aagaaatgca agcgaaagaa
    59641 ctttgaacga catttgcaag acttggagca tatctgaaga atggcttaag catggagttg
    59701 gagagatgtt ttttatcaaa gaattagatg agcaatttgc ccatcttgtt ggtgaactaa
    59761 gtaaaatgga aaatgaagta gaaagagaaa ttataactaa aatgttcaaa ttgaataaaa
    59821 aatatctgac tgtatttgat aaaatgatag attccttact agaaatagaa gaatagcaat
    59881 tgctattctt ctattttttt aaaagtccaa ggatataatt ttttatcatc aataaattcc
    59941 ttttatcttt atcaatattt aattttttta taatttcaat tatttcatgt ttttcctttt
    60001 ctaaatccat cataagttta tcccccatat tttcaaaata ttctgattta tatttttaca
    60061 attacattgc ttctgaaaaa taacagactt ccccctttta caatttttaa atttttatca
    60121 gatactaaat taatgttttt tgcttttgta tgtcatgccc tagatgagtt tgaaaattta
    60181 attgtattca atattgaaaa ttaagatttt tgattatttt taaaaatatt ttacgtacgt
    60241 agttttatat gtattatttc gatatatatc ttctttatgc tacaattgta acacctaatt
    60301 tgacattaag caaatgtcaa aatttaccat ttttgattat tttttaaaat ttaattttac
    60361 aaaaatagaa ttgtatacaa tcaatataca aagagaaaaa tttatactta tatttctttt
    60421 tatattgata aattttgata atattttatt ttttttactc ctgcttaatt tttttttaaa
    60481 gaaaaattaa ttggtaaaaa ttggtaaaac atttttagac atttagtatt gtgtagttaa
    60541 ctctattgtt atagtatttt agatatactt ttagaaggtg gtgtacttta tgaagataaa
    60601 aaagattcca gaaagtgaat tgttggttat gatgtttatt tgggacagcg aagataaaga
    60661 agttgcatct actgaaattt tgaagacact aggcgaaaag tacgaatgga aaaaatcaac
    60721 tatgttaact ttcttgagga gattagttgg tagaggattt ttagaggttg ttaagaaaga
    60781 tagatttaca tattataaag cattaataga aaaagaagaa tatttaaaag ttgagactaa
    60841 aagtttcttt agtttctttc acaaaaattc ttttgaaagt tttataagtg ctctgcatga
    60901 tgatgaagaa gtgtctgaaa ataaattaaa aagtttagaa aagtttattg aggattggga
    60961 agaagataaa tagagagcca atataatttt atttggttct ctatttatat atttgacctt
    61021 ctatttccat ttttaagcaa atttgtatct ttttaggtaa ataacatcaa gtttgtaatt
    61081 aaaacacgtc agacaagcct ttaaatgctt ttaagctata tttaataaac tttattttta
    61141 tcaatttcct ttttccactc atcaaaatta caaatccgat aaacttttct tatttttgga
    61201 acttctctaa gtttttcttt gtcatattca tatacattta ctttttttgt ttcaatgtat
    61261 ttattttctt ctaaagtttt aacagaagaa gttatgatat ctaaattatt tttactttta
    61321 gaacttagtc ctatattttc tgctataaaa ctattagttg caggtttaaa attagtttct
    61381 gtagtgattg ttttaagtaa aaaatatact tttattgtat ttgatttaaa tttattgacc
    61441 atttttttaa gcatttcata atttatcaat gcaaatttgt ttaaatcaat attattttct
    61501 gatggtaaac aatatacaat tccatttagg gtattttcta tttttagaat tttaaaatct
    61561 aacttctcta gtttttttat atttctttgg attgttgctt tatttacatt cagttcattt
    61621 gctatttctt gaatatcaag tttattctta tacaaaattc tatttttatg ggtattaaaa
    61681 atgtcactat tttcagcact ctttattaat agtgctccat attcttttat ataatattgc
    61741 ttatctttta aacaatatgt tgctattggt aagtaaattt ctttctgaat ttgttttctg
    61801 ttttcaagca taaaaaattc ttcatctcca atcttttata agaaattatt tttagtaagt
    61861 gtttaaagta tactagaaaa ataaatacta aagtttttta atataaaaaa agaatagttc
    61921 agaacatctt ttctagtctt taatagtttt taaaacagtt tttaaagtct ttaatagttt
    61981 ttagacaagc tataataatt ggtatatcta ggtttcagag atgaaaggtc atcattttaa
    62041 gggtcaaagg tcatcatttt aagggtcaaa ggtcatcatt ttaagggtca aaggtgagcg
    62101 tctttcaaaa atggttgacc tttttataaa ttaggtatta taatttatat gaggtgatag
    62161 attttggaaa aaaaagaaat tttaatgcaa ccaaataatt taataaaaag taaatatgat
    62221 tttacaaatg tggaaaataa attattttac aaaatacttt ttaatgctca gaaacagcaa
    62281 aattcatctt atgttacaac tatatcaaaa gaagaactaa aagtatttat gaaaaacaat
    62341 aatgattatg agcataaaaa tataaaagaa attttgaaca tgtttcaaca aagtgttttg
    62401 gaatttgact atatagaaga gacaactggc aagttaaaaa cttttggaag tggtttaatt
    62461 aatacatatg aattggatca tacagaccaa atttatacaa ttatgatgca tgaagttcta
    62521 tacaatcata taactgattt tgtaaaaatg cagaaaaaga aaaatggata tacagctatt
    62581 aatttatcag tgttatttaa ttttagaggt gcatatactc agaggttata cactttattt
    62641 agactatgga gcagagaaaa taaggaagtg gagataaaat ataagttaga tgaattaaga
    62701 ttttatttga aattaaaaga taatgtatat ccagagtata aatattttaa acaaaatgtt
    62761 ctaaaaagag caatgaatga aataaataaa aaaggcaaca tggttgtttc aataaaagaa
    62821 gaaaaaagaa aaaatagaaa aatagatgaa ataatttttt ctgtgataga ttatgaacct
    62881 agaaaatatt ttgataaaga tatattggtt gaagattata tacctaaaaa gaagcaggac
    62941 aatgaagtgt cttttagttt ttatatacca aatgaaaata tttttacaac agggacgatt
    63001 aaactattta aagaagattt taaagagttt gattttaaga aagatgttta tctgaatgct
    63061 tttaataagt ctattgctat agcatttgaa agagataata ctgatattat aaatacaaag
    63121 aattatgatt tttttaaatc aacactttca aataaaataa gtgaagttat aattcatcaa
    63181 aaagatgatt taaagtttaa agaagaattg gataagtatt gggatgatga agatgttaaa
    63241 aaagaagaaa gagaagttga ttatagcaaa gatcctgtca gacttgaaac tgttaggaga
    63301 aatttatttg atcaaggtct agtaaatgaa gaaggatatt actataaata ataattggag
    63361 aaaataaaaa atgtattatg tttataaata tattaatcta gatacggaag aatgtttata
    63421 cgttgggaaa acagagaata tctgcgatag acacgctagt catttaagta ataaaaaaga
    63481 aaattggtgt aataagaatt taagatttga gtatatagaa ttagataata aatatactat
    63541 ggatttttat gagatttact taataaataa actagagcct aaatttaata ttactggaaa
    63601 aggagaaatg gatatcgcta aaacatcatt ttcctataat ggtcaatggg taatttattc
    63661 agaaagagac ttgatgtcga gtttagcatc aaagagatgt ggaataaact atgaagtgaa
    63721 tgctaagatg ttaggcataa tgagaaaatt aaagaagata agcagcaatg aagaaatatt
    63781 tattggaaat gaaaatataa gaataaaata ttactttgaa acagtactag aaggaattaa
    63841 tttagaacca tgcatattga gtgtagcata taagacacta aaagaaagta cagttggaac
    63901 agtaatctca gtaaaacgtg aatatttatc agaaaatgta tgtttaatca ttgatagtat
    63961 ttttctaaat gaaataatga aagatccttt aatgtctaaa tcagatatta aagatttaaa
    64021 tagccaagtc aatgatattc ttaaaataat aggggttgat tgtgagtgga acaagctttc
    64081 tgattattgt agtgcaaact cttaactgat attacaaaat atggtataat taaatttagc
    64141 aagaagagcg taatatacaa tctatagagt ggagttcatg tacaaaagat tatcctccca
    64201 acgattcgag gggaggtgag ttacatggat aactttttgt ttaatgtttt agctagttta
    64261 acagctagtg tggtagttta cttaatcagt gaactattca aaaaagcaaa aagccactct
    64321 cgtgcaaaga gtgacttaca ggttgaattt aaatttatat ttaaattcaa aaaataaact
    64381 acgtttatta tgaactccac tctacgccta aatagattgt agttcttctt gcttttatta
    64441 tatcacaaaa tctttaaaat gatacaattt tatatcattt cttattatta agaaatagaa
    64501 aaacacttta attgattaaa gaataatttt atataatctt gtaaaaatat aaaaagaaca
    64561 cttaatatat tagcttgaaa aatattaagt gttctttttt tctattctat ttaaataaaa
    64621 tagaatagaa aaaatgattg aaaatattat tctatcatga tagaatgaaa aaaacagaat
    64681 attctaccgt gatagaatat tctgttttga agaagaagta aatattattt gaaggttaaa
    64741 aatataagaa caatggaggg aattatggaa gtaaacctac atacaggaaa agatgaattt
    64801 tttaagagcc atgaagtagc aaaaaactat ataggtggat tatataaact tattgatgtt
    64861 tacaaattct caatggaatc agtttccgtt ttaactagaa tagatttaga taagttaaat
    64921 aatttctatg ctggagaagc tagtttaaac tatgatgaat tgtgcttaat agaaagtgta
    64981 attataactc ctttggtagg agcatttgaa actgctgagc ataattacaa tatgattatt
    65041 aaggcacgta aagaaaggaa actgtaattg agaataatat aaaaaaatag ggggaaaaca
    65101 aatgaaaaaa acaattgctt tattactaac gctttgcgca gtaatgattc catttacagc
    65161 ttttgctgat ggaaatgatt atgaaggtac tggaaattca caaacagttg aattaattgg
    65221 ccaaccaaac tcagattcgc cttcgactaa atctgttgtt gttccaggtg gtactgctaa
    65281 tttaactgca atgagtggtg ggcagttatc ttggagagta acatctacgc ttggaaatat
    65341 tttaggattt gaaggaaata tagatgtata taaggctggc aaaattgtat catctcagta
    65401 tgtaggcttt agattagctg gaactactaa aactggtgta gtaactttta aagggctaaa
    65461 aaagggttca tatattgcta aatttaaagg aaaaggtgtg tgtacaggag gtattatatt
    65521 attctctagt ccagctgaag cttcttttac aattagataa ttataaaaag tagaaaagag
    65581 tgtttagaaa tatatgaaac actctttttt tatgcaaaat tataaaaagt gtgtttttaa
    65641 ttatatacaa aatgataaat tttataaaaa attataaaat tacgtaaaaa attataaaat
    65701 attataaaaa atatcttata aatctattga attactgtgt aaaattt
//
LOCUS       DAYATN010000007        59007 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.007, whole genome shotgun sequence.
ACCESSION   DAYATN010000007 DAYATN010000000
VERSION     DAYATN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.007"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..285)
                     /locus_tag="ACGI8A_000466"
     CDS             complement(<1..285)
                     /locus_tag="ACGI8A_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178478.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIV"
     gene            complement(392..895)
                     /locus_tag="ACGI8A_000467"
     CDS             complement(392..895)
                     /locus_tag="ACGI8A_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFH0178479.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            complement(947..2146)
                     /locus_tag="ACGI8A_000468"
     CDS             complement(947..2146)
                     /locus_tag="ACGI8A_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HFH0178480.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            complement(2406..2594)
                     /locus_tag="ACGI8A_000469"
     CDS             complement(2406..2594)
                     /locus_tag="ACGI8A_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178481.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            2929..3897
                     /locus_tag="ACGI8A_000470"
     CDS             2929..3897
                     /locus_tag="ACGI8A_000470"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFH0178482.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            complement(4014..5144)
                     /locus_tag="ACGI8A_000471"
     CDS             complement(4014..5144)
                     /locus_tag="ACGI8A_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFH0178483.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(5297..6250)
                     /gene="msrA"
                     /locus_tag="ACGI8A_000472"
     CDS             complement(5297..6250)
                     /gene="msrA"
                     /locus_tag="ACGI8A_000472"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFH0178484.1"
                     /translation="MIKLATFSGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            complement(6374..7279)
                     /locus_tag="ACGI8A_000473"
     CDS             complement(6374..7279)
                     /locus_tag="ACGI8A_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFH0178485.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            complement(7651..9243)
                     /gene="hcp"
                     /locus_tag="ACGI8A_000474"
                     /gene_synonym="priS"
     CDS             complement(7651..9243)
                     /gene="hcp"
                     /locus_tag="ACGI8A_000474"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFH0178486.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(9303..10028)
                     /locus_tag="ACGI8A_000475"
     CDS             complement(9303..10028)
                     /locus_tag="ACGI8A_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0178487.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASFLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            10193..10909
                     /locus_tag="ACGI8A_000476"
     CDS             10193..10909
                     /locus_tag="ACGI8A_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFH0178488.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            complement(11034..11609)
                     /locus_tag="ACGI8A_000477"
     CDS             complement(11034..11609)
                     /locus_tag="ACGI8A_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0178489.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNHYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(11783..12205)
                     /locus_tag="ACGI8A_000478"
     CDS             complement(11783..12205)
                     /locus_tag="ACGI8A_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0178490.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            complement(12219..14396)
                     /locus_tag="ACGI8A_000479"
     CDS             complement(12219..14396)
                     /locus_tag="ACGI8A_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFH0178491.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYKQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEANCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            14677..17856
                     /locus_tag="ACGI8A_000480"
     CDS             14677..17856
                     /locus_tag="ACGI8A_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFH0178492.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGINKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(18141..18905)
                     /locus_tag="ACGI8A_000481"
     CDS             complement(18141..18905)
                     /locus_tag="ACGI8A_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0178493.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(19061..19348)
                     /locus_tag="ACGI8A_000482"
     CDS             complement(19061..19348)
                     /locus_tag="ACGI8A_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178494.1"
                     /translation="MLCYRNEVAKNTIEVVKDLKAEDMKRKVSPIDLERILVTGGVIK
                     HEDSIWLLDFWGNKDIAGILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(19368..20861)
                     /locus_tag="ACGI8A_000483"
     CDS             complement(19368..20861)
                     /locus_tag="ACGI8A_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFH0178495.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            complement(21189..21731)
                     /locus_tag="ACGI8A_000484"
     CDS             complement(21189..21731)
                     /locus_tag="ACGI8A_000484"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0178496.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            complement(22021..22968)
                     /locus_tag="ACGI8A_000485"
     CDS             complement(22021..22968)
                     /locus_tag="ACGI8A_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178497.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGYIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            complement(22996..23487)
                     /locus_tag="ACGI8A_000486"
     CDS             complement(22996..23487)
                     /locus_tag="ACGI8A_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178498.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            complement(23930..24823)
                     /locus_tag="ACGI8A_000487"
     CDS             complement(23930..24823)
                     /locus_tag="ACGI8A_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFH0178499.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            complement(25048..27333)
                     /locus_tag="ACGI8A_000488"
     CDS             complement(25048..27333)
                     /locus_tag="ACGI8A_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFH0178500.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLLYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKCIKIDK"
     gene            complement(27330..28010)
                     /gene="pgmB"
                     /locus_tag="ACGI8A_000489"
     CDS             complement(27330..28010)
                     /gene="pgmB"
                     /locus_tag="ACGI8A_000489"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFH0178501.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            complement(28234..30117)
                     /locus_tag="ACGI8A_000490"
     CDS             complement(28234..30117)
                     /locus_tag="ACGI8A_000490"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFH0178502.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            complement(30375..31196)
                     /locus_tag="ACGI8A_000491"
     CDS             complement(30375..31196)
                     /locus_tag="ACGI8A_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HFH0178503.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            complement(31387..31740)
                     /locus_tag="ACGI8A_000492"
     CDS             complement(31387..31740)
                     /locus_tag="ACGI8A_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178504.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            complement(31782..33224)
                     /locus_tag="ACGI8A_000493"
     CDS             complement(31782..33224)
                     /locus_tag="ACGI8A_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFH0178505.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            33900..35111
                     /locus_tag="ACGI8A_000494"
     CDS             33900..35111
                     /locus_tag="ACGI8A_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFH0178506.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKFLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            35203..35718
                     /locus_tag="ACGI8A_000495"
     CDS             35203..35718
                     /locus_tag="ACGI8A_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HFH0178507.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(35985..38084)
                     /locus_tag="ACGI8A_000496"
     CDS             complement(35985..38084)
                     /locus_tag="ACGI8A_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HFH0178508.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMEKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(38208..38747)
                     /locus_tag="ACGI8A_000497"
     CDS             complement(38208..38747)
                     /locus_tag="ACGI8A_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFH0178509.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            complement(38750..39490)
                     /locus_tag="ACGI8A_000498"
     CDS             complement(38750..39490)
                     /locus_tag="ACGI8A_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFH0178510.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            complement(39492..40541)
                     /gene="vorB"
                     /locus_tag="ACGI8A_000499"
     CDS             complement(39492..40541)
                     /gene="vorB"
                     /locus_tag="ACGI8A_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFH0178511.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            complement(40558..40776)
                     /locus_tag="ACGI8A_000500"
     CDS             complement(40558..40776)
                     /locus_tag="ACGI8A_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0178512.1"
                     /translation="MNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAHVEVDES
                     KCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            complement(40809..41912)
                     /gene="hisC"
                     /locus_tag="ACGI8A_000501"
     CDS             complement(40809..41912)
                     /gene="hisC"
                     /locus_tag="ACGI8A_000501"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFH0178513.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            complement(42015..43445)
                     /locus_tag="ACGI8A_000502"
     CDS             complement(42015..43445)
                     /locus_tag="ACGI8A_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0178514.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            44103..45467
                     /locus_tag="ACGI8A_000503"
     CDS             44103..45467
                     /locus_tag="ACGI8A_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFH0178515.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            45491..46144
                     /locus_tag="ACGI8A_000504"
     CDS             45491..46144
                     /locus_tag="ACGI8A_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0178516.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(46263..47045)
                     /locus_tag="ACGI8A_000505"
     CDS             complement(46263..47045)
                     /locus_tag="ACGI8A_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0178517.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(47162..48328)
                     /locus_tag="ACGI8A_000506"
     CDS             complement(47162..48328)
                     /locus_tag="ACGI8A_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFH0178518.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            complement(48467..49831)
                     /locus_tag="ACGI8A_000507"
     CDS             complement(48467..49831)
                     /locus_tag="ACGI8A_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFH0178519.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            complement(50143..50667)
                     /locus_tag="ACGI8A_000508"
     CDS             complement(50143..50667)
                     /locus_tag="ACGI8A_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFH0178520.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            complement(50717..51214)
                     /locus_tag="ACGI8A_000509"
     CDS             complement(50717..51214)
                     /locus_tag="ACGI8A_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0178521.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            complement(51447..52718)
                     /gene="hflX"
                     /locus_tag="ACGI8A_000510"
     CDS             complement(51447..52718)
                     /gene="hflX"
                     /locus_tag="ACGI8A_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFH0178522.1"
                     /translation="MAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSITQN
                     AKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRTAL
                     ILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKLEL
                     DRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLVDI
                     FKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRSTL
                     EEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVLDR
                     ILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNENTS
                     IINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            complement(53443..55275)
                     /locus_tag="ACGI8A_000511"
     CDS             complement(53443..55275)
                     /locus_tag="ACGI8A_000511"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178523.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(55268..57514)
                     /locus_tag="ACGI8A_000512"
     CDS             complement(55268..57514)
                     /locus_tag="ACGI8A_000512"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178524.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(57582..58043)
                     /locus_tag="ACGI8A_000513"
     CDS             complement(57582..58043)
                     /locus_tag="ACGI8A_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFH0178525.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(58348..58914)
                     /locus_tag="ACGI8A_000514"
     CDS             complement(58348..58914)
                     /locus_tag="ACGI8A_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFH0178526.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT        19545 a        10011 c         6324 g        23127 t
ORIGIN      
        1 aactataact acagttaaaa gaagtctagc atataaaaaa tttttttctt tcaacataac
       61 tcctgcaact ccaaatataa aaaatagaga agcaataaat tttccaataa gattattatt
      121 aggataataa ctaaaaagca taaaacaaag tacaaatgta agtactccta ttgcattaaa
      181 cacaatttta ttagatgcta tttttccttt attgaaaaag aaacagaata ttgacactaa
      241 atctaaaaat aaaaatacat aaaacattcc ttttaaaacg cccattttta taccccctaa
      301 aaattctaat tataatatcc agtttatttc atataatcgt caatataatc aatactttat
      361 aacatcagta taacagtatt acaaatttat attacgaatg tactacttcg tattcataca
      421 tattataatc acactcatac atatttaaag actcatatgt tgcttttgca tttatgtttt
      481 ctttttctac atataatctc attccaacta tattttcatc tttatcacat atatttttta
      541 tataattaaa taaataatta aagatacctt ttcttctata ttccttatct acatatacac
      601 tctgaatcca aaggaaattt ccatttctcc aatcactcca ctcatatgta tacataatct
      661 gtgcaacaac tttatcaaat actgtatata catgatattt tccttttctc tcatctaata
      721 acaatgcttt tacaccttta gttaaaacat ccatatcaag ttcttttcct tcagtttctt
      781 tagccaaatt ataattaaat tttgctattg tatccaaatc atccatacgt gcttctctta
      841 tctctattag ttcttcattt tcatataact ctatgtagtc cttttgttgc tccatactta
      901 ttcctccata tctattttat ctactaaggt tttttatata atatatttat agagtttttt
      961 ctatcttcat aaatgcataa tcaagtgtag cctctaaatt tccattattc ttacctcctc
     1021 cttgagctaa tactttactt ccaccaccct tgccatctat aagatttatt gcatccttta
     1081 ataaaactcc catattaata tcttttaaat ttttagaaca tgcaaaaact aaatttattt
     1141 tatcattaaa tttaacagct aataatgcaa tactattttc atattctgca attttatttg
     1201 ctatcttatt tacatatttc aaatcttcat catcataaat ttttttaatg accgaaatat
     1261 tgccaacctt atttgctttt tcaatcattt ctttgacctg atatgtagat actacttctt
     1321 caagccttct ttttttaatt aaagcctctt ccactttact atttagattt acaataccat
     1381 ttatagcatc ttcctcacta cagcttaaat atctacatat tttagtcaaa tatatatccc
     1441 tcttcaatac ttcatccact gcccttttac ctgctagaaa ctctattctt gtacttgact
     1501 tatgtttttc ccatttcttt atttttatca ttctcaattc accagttgat tttggatgca
     1561 ctccacaaca tgcattcgta tctaaatcac ctatttttat tactcttatc tcttcatcag
     1621 tatttggcaa atcacgtctt aaccaaatct tttttagttc ttttttcgtt ggaagtaatg
     1681 tctctacttg tatattttga cgaataattt cattggcaaa caactctatc tctctaattt
     1741 tatcctcttc taaattacct tctatatcta ctgtactata ctcatttccc aaatgaaaac
     1801 ctgtcgtatt tgcattaaaa gttttaaaaa aacatccaga caatacatgt tgtccaaaat
     1861 gttgatgcat tccatcttct cttctatccc aatcaatact acactttacc ttatgtatct
     1921 ttataggttt tttatctaat acatggtata ttttttttcc atcttcatac acatttatta
     1981 catcatcatt ctctattttt cccaggtctc caaactgacc tcctccacca ggaaaaaaag
     2041 ctgtcttatc taataaaaca tgatacttat tttctacttc ttttatttca attatttctg
     2101 ctgtaaaatc tcttatatac tgatttgtat aatataattt ttccattata attctatctc
     2161 cattttctta attctagatt actttacttt ttagatacaa atttcaaact aagcttaatt
     2221 taattgtatt aaaatagaac tcatcctatt atttactaac atcttctact atattttaac
     2281 atatagatat taatgttaac aaatttataa ttgtatatta tttcaatatt ttataacatg
     2341 tatgtttact tattttctta tataataaaa tgtatattaa aacatgttaa attgcaaaat
     2401 aagcttcaat ctcttttact aatttcatcc attattaatg attttgaaat atacattact
     2461 tctataatat tcttaaaacg tgtatgatga gatgtctcct cagaaaattt cttctgtcct
     2521 tttccacaat taataatact tgaagatact actctcagtg cttccaccaa ttcttctttt
     2581 gtatgataat tcataacaca tttactcctt tattttttaa tttagagaaa cagtatatct
     2641 caatttaata tcattataaa ctgacatcat tattatttat acacatcttt tagcacttaa
     2701 atattctaac tatcagtgac attacatatt ttaataatta ttaatataca tataatactt
     2761 taatattcac taatagagag tatattaaca ttgctttttt aaaaatttta gtataaaatc
     2821 atattaaata aattttctga aataatagaa tattaaaata tccaaaaaca gcttttaata
     2881 acatctataa ttttcaatgt atcgttattt tagggggagt gatttaaaat ggcaataaag
     2941 ccaagaaaaa tatctattgt aggttcaggg catgttggtt ctcattgtgg atttagttta
     3001 ataactcaag gagtttgtga tgaactcttt atgattgata tagatgaatc taaatcaaaa
     3061 gctcaagctt tagatttagc tgatgcagtt tcatatctgc cacataaagt acatatagaa
     3121 aaaggaactt tttctgactg taaagattct gacatagtag tgataagtgt tgcagactct
     3181 tcagaagggc ctttacgtag acaaaacaca actagattag atttactaag accaactatt
     3241 ggaatgataa aatctatagt aaaacccata gttgattctg gctttgatgg tatatttgta
     3301 gtcatatcta atcctgttga tgtagttaca aattatattt gggaaaaatc tggctttcct
     3361 aaaaacaaag tcattggaac tggaacagct ttagattcaa caagacttag aaggatttta
     3421 tctgaagaaa ctggaatcgc tcaacaatca attcaagctt attctatggg tgaacatgga
     3481 gattctcaaa tggttccttg gtctcatgtt tcaattggtg gtaaaccaat cttagacatg
     3541 ataaaagaca acccaagtac ttatagcaat ttagacttac catcaatagt tgaaaaaaat
     3601 aaaaaaacag ggataagtat aataaatggt aaagattgta cagaatttgg tataggtact
     3661 gctttagtag aaattgtaaa agctatacta cacaatgaga aaaaagttct tccagtttct
     3721 acattactag aaggtcaata taacgaaaga aatgtatttg caggtgttcc atgtgttata
     3781 ggaaaagatg gtattgaaga aattattgaa atcaatatga ctgaatatga gcaaaatgaa
     3841 tttaataaat cttgttctgt tttaagggaa tatattgcac tttcaaaaac actttagatt
     3901 caatattctt tcttacacta aaagcaggta aatgacttat tgaaaagacc tttaactaag
     3961 ttaatgttta aatcatttgt acctgctttt aattttaata ctaaaatata aacctatttt
     4021 ttaccatcaa attttataac tttacttttt ttatttacct cttgagataa caatcctttt
     4081 ttcaatccca tcaataattc taagtcttct ggataaagat ttattccaaa ttcctctaaa
     4141 cttgatacta tactatttac actcattatt ttattttcta tcaataattc aactgctctt
     4201 tttaaatacg ttgggcttga acctttcatt ttgtctagtg gttcttctat caaccagcct
     4261 tttttatcca tttcattcat aagataattg tattttttgt aatttatctt ttctagatta
     4321 tatgctctaa aaatcatagc agatataggt acaatccatt ttttctttag ttctacataa
     4381 taatctaaat cttctggatg agtcaaatct tttaaaaaac tctcttttgg caataaaaat
     4441 gcacaggcat actcatcttc tgaaaattgc ttacttggta ttcttaattc acttgatatt
     4501 atatatccaa gctcacatgc caaagtataa tttctaattg atgctgactt tttgtcatta
     4561 cccagagata ttagatattt agattcttta ctaattctct gtttttgagt aaagatagtt
     4621 gccccttttc tatctacatt catacctgat attataatcc ctttagactc taacaaactt
     4681 accatattta ctattggagt actatttaaa tcccaaaact ctctagtctt atatgcaaga
     4741 gattctatat catttatatt aatatctgac attacttttc tatttggcaa gttttctttt
     4801 ggaaacttaa tataattctc cataaatgaa tatattttat gtattataat tattttttct
     4861 ctataagaaa tctcttctac tctaggtagt ttagattgag ggttaaagtg gctatcctct
     4921 acaacgatat ttatattttc atttttataa aaatactctt ttggaaaatt taatatatta
     4981 gataatttta atgtattttc tatatttggt acatacttat tttcctcaaa agcttttaaa
     5041 tcttttttat ttattttagt ttctttagac aactgttcta tcgtctttgc tctaaaaatt
     5101 ctagctgatt ttaatctctt tccattaaag tcttttaaat tcatttgttt tctctctttc
     5161 aaaattaaat tcttaatcta ttggtataaa taagattaca attaataatt tatcatatta
     5221 ttgacaaaaa aacaaaaggt gaaaatattt ttttatattt tcacctttta gataagttta
     5281 taataaatat tcctaattac tcaaaaagtt ttaaatattc tccatatcct aaagcttcca
     5341 tattttctct tggtataaat ttaagagaag cagaatttat acaatatctt aaacctccaa
     5401 gttcttttgg accatcatca aatacatgac ctaaatgaga atttccttta gcacttctta
     5461 cttcaactct gtacatacct tgtgagaaat cacttctctc aatgatagat ttatctataa
     5521 ctggctttga aaaacttggc cacccacatc cagaatcaaa tttatctttt gaagaaaata
     5581 acacttctcc acttacaata tcaacataaa ttccctcttt gttattgtca taatattcat
     5641 tttcaaaagg tacttctgtc atatcatttt gagtgacttc aaattgtata ggagttagag
     5701 tcttttttaa ctcttcccta tttatattgt ttccatccca atggcttttt acaaaattat
     5761 atcttccaga ccctttataa tacatattgt agtttaatgg tcttttctta taataatcct
     5821 gatgattttc ttctgcttcg tagaatttag ttgctggaat tatatcagtc actattttac
     5881 tatcaaatat tttactattt tccagatgtt ctcttgactc taaagctata tttttttgtt
     5941 cttcatcatg ataaaatata gcagtcttat attgagttcc cctatcacca aattgtccac
     6001 catcatctgt aggattaatc tgtttccaga agatatttag aagttcatca taacttatta
     6061 tatcatcatc atatgttatt tgaattgctt ctaaatgacc agtagttcct gagcatacct
     6121 cttgatatgt agggttttca acatgaccac ctgtatatcc agaaactact ttaataactc
     6181 catcatactt atcaaaaggt tttaccatac accaaaaaca tccacctgaa aaagtagcaa
     6241 gttttatcat tttcatcact ccttaaataa attatacttt atttattccc taagaagtaa
     6301 cttaataatc tgcattttat tatttttacc tactcaatga aatcatgtgt aaaaatacaa
     6361 ttgcaaattg aatctaattt ctataaaagc tctttttctt tatccatcca aaagcatctt
     6421 ttatatcaca attttctcct aaagacatta gatattctaa tgcatcacaa tcatccatag
     6481 ctacatcttt ttccatctta ggatttaatc tatagtaatt atctccaaga agtttttgag
     6541 aaaataactg actcatttga gaatttcctt ctaaggtaat agatataatt ggtaaatcgg
     6601 gttctttatt aattatccag tcaatagctc cccattcggt agtatctgcc tttatactgt
     6661 tatatacata tccagtccct attgaaaaaa gtcttgtatt cttaagttta aatccactgt
     6721 caatggcatg aattatacac gctaaactcg ggtcagttgc tattattcct ccatcaacat
     6781 gacgattata tgttggaaaa aataccggag cagcactact tgccattgct acatctacaa
     6841 ctttgctagt ctttgtaaaa gaatttggca tattgttata aaacactggt ttccatgcat
     6901 tttcctcatt tcctatataa aaacttggta tcattactaa tttatttaaa tcttttaatt
     6961 ctagttcctc tggaaatata cttaataaaa cttcttttaa gtttttgttt tcatactttg
     7021 gtctgagtat ttctgcataa cttttattaa aaatatactt tgaattttca attgagtaaa
     7081 gttcttttat ctctttaggg cttacaccat acgcaagccc tagtgcaatt agactccctg
     7141 tagatgtacc tccaaacata tttatattgt ttaatagatt tggatactct tttactatcc
     7201 tctcaagtat ggatatactc aatgcccctt ttaatccacc accatcaaat gccaatatat
     7261 taaaattaga atttttcata tacatatacc cccacataaa acaccttata ttttaaattt
     7321 taatcaaaat aatttcacta tagtatatat attttatgca agttacatat tacaatgttc
     7381 ctcttttaat atttaagtta cattcaaacc tttaatactt aaaataaaat atataagata
     7441 ttatatatct aagtattata aatttaatac aaagattctt aaaaagaata tactgaaagc
     7501 aggtttattt ttcaattatt acaaaaaata aataaagggt catctcaaaa ttgatttttt
     7561 aatcaaattt taagtttaac cccttatctt aattgtggca tacttaaaaa tattttaact
     7621 ataaatatct ttaagtatag atatgtttat ttatcctaaa atcttcttca tatcttcttc
     7681 aggagtagat ataggtgtca atgcaaattt atcaaccaat atatctaata cattcttaga
     7741 taagaatgct ggcaaagaag gccctatata gatatttttg atacctaaat gaagtagtgt
     7801 aagaagtata caaacagctt tctgctcata ccatgacagt accatgctaa gaggcaaatc
     7861 atttactcca cattcaaaag catttgccaa tgaaactgcc actttaattg cactataagc
     7921 atcattacat tgtcccatat ccattaaacg aggtaagcca cctatatccc caaggtctaa
     7981 atcattaaat ctatattttc cacatgcaag tgtcaatata acagtgtctt taggagcttg
     8041 ttttacaaat tcagtgtaat aatttcttcc aggttttgca ccatcacaac ctccaaccaa
     8101 taaaaagtgt cttatagctc cactctttac agcatctact actttatctt gtacagatga
     8161 aactgtacct cttgcaaaac cagtagttaa tgtatctcct ccattaatac cagtcatatg
     8221 aacatcttct ctatatcctc caagttcaag agctttttct attacactag aaaaatcttt
     8281 atcttcatca atatgaacca tacctggata agcaactact tctgtagtaa atactctatc
     8341 tctataacta tctttaacag gcattaaaca gtttgttgta aataatattg gagcaggaat
     8401 attatcaaat tctttttgct ggttttgcca tgctgtacca aaattacctt ttagatgttt
     8461 atattctttt agttttggat atgcatgtgc tggtaataac tcaccatgag tatatatatt
     8521 tataccctta tctttcgttt gttctaataa tttgtgtaaa tcataaaggt catgtcctgt
     8581 aactacaata aatggccctt tctcgatttt catagatacc tgagtcggtt ctggtgttcc
     8641 ataagtttca gtatttgctc tatcaagcaa ttccatacat tttaagttta tttctccagt
     8701 tttcataact agacctaaaa gttcgtcaat agacaagtct cctccaactg ctattaatcc
     8761 ttcatagaaa aattcattta cgtctttgtc tgtataacca agtaccattg catgatatgc
     8821 ataggcagcc atacctctta atccaaataa gattaatgat tttaatgaac gtatgtcctc
     8881 attatcattc cataaatcct tcatatcata gttatcattt ctattacaag ttgttgaaca
     8941 atctgaacat ctaggtacaa gtttactttt ttctacctca gttttttcaa tttgtctttt
     9001 tatagcttca tcatcaaaac ttacattcgt tatagtagtg aatagcccct ctatcataat
     9061 tttatctgtt tcacttgttg gtctattctt atttccaata gttgctcttg aaagaccaat
     9121 caacactcct gtaagttcgt cttgtaagtt agctgtaccc ccctttttac cacagacacc
     9181 atttatagta caacctttac ctcctgctgt ttgttcacat tgaaaacaaa acattttttc
     9241 cattttttat tcctcccttt ttatttatta aaatttatta tatttttcta taattcactt
     9301 aattaaattt ctccatctac aatttttcca tctacagata tagtaactat ttgccatggt
     9361 atcatttttc cactattttg tagagctgtc ttaacagcat ttactatacc accacagcaa
     9421 ggaacttcca tacgtgttac tactatattt ttaatatcat tttcttttaa aatctctgta
     9481 agtttttcag catagtcaac ttcatcaagt ttagggcatc cgattaatgt aaccttattt
     9541 ttcataaatt tattatgaaa atttccatat gcatacgctg tacagtctgc tgctattaaa
     9601 aaacttgcat tctttaaata tgatgcatta gttggtacta attttatttg tactggccat
     9661 tgattaagtt gagaaccttt tatctctact atatcattgc ttatagacgt actatttgaa
     9721 acctctctat ttattgactt agactgagaa cctggacatc cacatgctaa agttttcttt
     9781 tgtgctttct ttgcttccat atttgctttt actgcagctt catcatattc tgctgcttct
     9841 ctatactcaa atgaaatcgc atttgttgga caagttggca gacaatctcc cagcccatca
     9901 caatagtcat ctcttaaaag ttttgctttt ccatttacca ttcctatagc ttcttcatga
     9961 caagcttcaa cacatagtcc acaaccatta cacttttcct catcaatttt aattattttt
    10021 ctcatcattt atatcacctc ataaaatttt tttaatttaa agtattgatt tcttatatat
    10081 ttatttttaa tacctatatt tctttatttc aaatttcttg tagtaggatt tttatatccc
    10141 cttgctttta taaacttagt atagtacaat gaaaacaaat tatcggttgc atatgcaaca
    10201 aggaagtgat tatttgaaaa aatttttaga aaagcatata gacttactta aaaaaattcc
    10261 tctatttgaa ggaattaaaa gtgatgaact tgaagaaatg ttggaatgtt taggtgttgt
    10321 agataagact tacccaaaag atagtataat attctctgtt ggaagtgaag ttacttctat
    10381 tggaattatg cttaaaggaa gtgctcatat aataaaggaa gatatcgaag ggaataggaa
    10441 tattgtagct gaactcttgc ctggagattt atttggagag gtattcgcat gtactagact
    10501 acataaaagt aatgttacag taaccacaac atcttcttgc gaagttttat ttataaagtt
    10561 taaaagtgta actggtattt gttcatctgc atgtgtattt cataataggc ttgttgaaaa
    10621 tatgctacaa ttaatagctg aaaaaaacat tttacttcac aataaaatag aattactttc
    10681 tcgtaaaaca actcgcgaga aattgcttat gtatttttca aaacagatag aacaaacagg
    10741 tagccaccag tttacaattc ccttttcaag aaatgaaatg gctgattttt tatgtgttga
    10801 cagaagttct atgtcaagag agcttggcaa gatgcgagat gaaggcttgt tatcttttaa
    10861 caaaaataaa tttgaaatct tttattttca aaacaagtta gatatctagg cttgtataat
    10921 atctgtattc aaataatatt gttttttatt agttattttc tagaaacctt tatacaaaaa
    10981 aggagtatta ataattaatg ctcctttagc tatactataa attaaacttt tatctatcca
    11041 ttgatatcaa tggctatttt cattccaagt ttataggctt ttttcaatac cttaaaattt
    11101 tttttagtgg aatttctttc aaatgctttg gttacaacta tagttttgta gtgatttaat
    11161 cccatgccct ttaaatgttt tgcagtatac ttaaaatatt ttttaaataa aaacgaaata
    11221 ggagctgctt gagtatagac catgattaac tttttttgac caaacctagg tatatgttta
    11281 gacttattta tctctgttaa aggatacaat ctatctaaaa gtagctttgt ttgagctgat
    11341 acctgacata tatatactgg cgaaccaatt attatatatt cagcactttt tatatcttca
    11401 tatatgtcaa tcatatcgtc tttaaatgca caagtaggaa cttttctaca gtatagacag
    11461 ccttgacatc ctttaatatt catatcattt agataatatt ttttaacttc aacattatta
    11521 tcatttacac cttttattat ctcatcaagt attgtgtcta cattcccttt ttttcttgga
    11581 ctgcccataa aggctaatac ttttaccata ttaagttctc ctgtctaatt tatttttcta
    11641 aatttaatct aaagcacact ttattctaaa aatatatttt atatactttg tatttgtttt
    11701 atttacattt tatattacaa aagtatatca aaaaataatt cttaacacaa ttatttaaaa
    11761 gtagtatagc aatttaatac aattatttat taaattttaa tgctcctgta gggcatacca
    11821 ttacacaagg tgaatccata ccacacttat atctctcaat acaaccatta cacttatttg
    11881 ccttaccatc atttccataa attaaggctc catattcaca agctttttca caaagtttac
    11941 accctataca gttttgattg tctaatacaa tgaatccttc ttctttttta aaacaatttt
    12001 taggacatac acctaaacat tttggctcat tacaatgtat acatgctaat gaaaaatatt
    12061 ctatattttc accttcctct attgtaaata catgtctaaa aagttcacca ttgtctatat
    12121 tattatcatt ttggtctata catgcaagca aacatgctcc acatgctgta cataattctt
    12181 tattaaattt aaataaactt ttcattatat ctctccattt atctattcat tttttcaatc
    12241 ttacacttac aacttctata cgcagggaat ccactataag ggtcaaaatt atcacaaata
    12301 atactattta tatcagcttc tctatatcca tgatacatat gtatattctt atataagact
    12361 ctatcagtaa cattgacttt tacatccaaa cttcctatct tagtagttat ttttatataa
    12421 tctccactga taacacccaa ttcttttgca tctaagctag aaatatcaca attagcttca
    12481 ttttttaata ttcttgaaag tttgatgtca tgaagtcttg aatgtagtgc atttgaaatt
    12541 cttgctcctg atgtcaataa atgagtatat tcatcatcta tattatcatt aaatcctcta
    12601 tatgttggta gtgcatccag cccaaaatct ttatattttt caataattgt agattttaat
    12661 tcaaacctag aagtgctagt atcataccct tctcttgtat atgaacctac tatatatggc
    12721 ttaaaatctt tacattttaa tggtaaatca ctttctttaa gtttatctac atctacacat
    12781 gtatttttaa acatatgtct tacacttgct tcataacctg cttttaataa atcatcatct
    12841 atattcatag catttgctaa atctgaaagt atttctacat ctgattttga atcgtataat
    12901 ttatcaataa cttttttggt atatgtaata taccctcctg gataactctt acattcttct
    12961 ctctcaaatg atgtacatgc aggtaaaagt atatctgcat attttgatgt atctgtcata
    13021 aataaatcta tattcacaaa aaaatctaca ctattcaacg ctttaaaaag tctccctgaa
    13081 ttaggaaaca ttctagcatt tatcccatga caaaacaaag ctcttatttc atatggtgtc
    13141 ttttcttcta tttgcctagg taaatccata gcttgaaatt gagtcaccat ttcattccat
    13201 agaggaaatc tttctccacc aattctttca tttatatttt taggtagaac ttcttttaca
    13261 aactcttttt ctagtgtttg gaatccacct atcttaccag caaagtaatc ttgttttggt
    13321 atgtttccac ctattctatc ataattacct gttatagcgg ataaagacat cattgctcta
    13381 taattttgaa atccattttg atgatgaact attggagctg cactttcatt tatacaaaat
    13441 ggaccttgtg acaacatctc aacagctttt actatattat ctttagaaac tcctgtaatt
    13501 ctacttacag tatctaaatc aaataatttt acatattctt tatattcatt aaatccatgt
    13561 acatgctttt ctatgtattc tctatctatt aaatcctttt gtataaatag attcgccatt
    13621 ccaagagcta aagctccatc agttccaggt ttaattctta aatgtaaatc tgctagtttt
    13681 tgagttgtcg gtgttattct agggtctaca attataaatt ttagaccttt ttcttttaat
    13741 tttattagat tcacactatt taaaaattta gagtagtatg gattaaatcc ccaccctaaa
    13801 taagtttttg cattctccat atcttgcatt gtaaagtttc ttccagtagc aacatttcca
    13861 gccatctgtg ctgatgttga acatgaacta gattctgtac cataattagg tgaaccaaat
    13921 gaagatgcaa acctttgtaa aaaaggtcta taccactttc cataacccgt aaaaaaagct
    13981 acatgctttg ctccatactc agattttact ctatttaatt tttctgctat ttctctataa
    14041 gcttcatccc atgatattgg tttaaatttt ccttcacctc tttttccaat tctttttaga
    14101 ggtgttttta ttctatcttc tctatagata tattctctat tgcaaagacc tttggtacat
    14161 aacaaccctt tattcattgg atgttctttt gtaccttcta tttttattac tttaccatct
    14221 ttaacatatg catcaattcc acaatgtact cctggcgaac aaatatcaca taaagtcctc
    14281 tttatttcta tacctgtttc cttacagggt attttagctt taagttgttt ataactatcc
    14341 atattaacat tttcctttcc caataataaa tacaaattat tatacttatt atacattctt
    14401 tttattcaaa tatcatactt tatattttat acagtgtaat ttctaagaat atttttaata
    14461 ataagatttt atatttatta ataatctatt tatataatta tttttcattc atagtttata
    14521 agataattca ataaaaatct aatagtaaat tgttatttac acatatatcg tgatagataa
    14581 aagctattca tatctgcctt aattttagtc ttaaatttag agtgtaaatt gaatgcattt
    14641 agattataat atatacagtg aggtaaggtg aataatatga aaatagatat gatatataaa
    14701 ctaataagaa gaaatacaga accatcaaga tggtcaagag gtgtaaaata ttatactaat
    14761 caattagttg attatataga catagatatt gatgacctac aaaataatct gttgacattt
    14821 cgtggtggag tcaattctga agataattat agcttttatt ttaattcaat aactatagat
    14881 ataaagcact ttaagataat tgaaaatgat tgtagttgcg ctgactgtca atcaaagggc
    14941 aaaagtattt ttatatgtaa gcatatagtt gctattgtga taaaaagctt aaaacaaata
    15001 aaaaataaaa aagatgttcc tcctattcta atagatatat taggtgcaga taaggataag
    15061 ctttctaata caaattctaa aaccagctta gatttaagca agcaattact ttctttgtta
    15121 gacaatacta aaagagaaag ggttaattta gatgtattga taaatgtgga ttcaaataaa
    15181 gcaattgctg aatttaaaat aggaattaat aaaatgtaca ttttaaaaaa cattactgaa
    15241 tttgcatatt caaaacttaa tggatatgaa ttagattttg ggaagaaatt tacttacaac
    15301 cccaacactc attattttgc tgatgaagat gagaagttag tagatattat agaagaatat
    15361 gggacaaatc tttcatacaa cttttcttca tcacattcaa aaaaatttat ggagataaat
    15421 gcaagctctt taaaacaatt tttaaatgct attaagtaca aggattttac ccttattttt
    15481 tctaaagagg aatatcatcc aaagatatta aatgatactc tacctgtaag gtttgatttt
    15541 aaaaatatag aagataaaat agtcttatat tctaaagatt cactacctgt tccacttact
    15601 aaaaaaaatg atgtactttt atatgatggc aatatctatt tattatcaca taaaaaagct
    15661 catgattatt caaaaatata tcaaatatta tcaaagacta aagagttaaa atttgataaa
    15721 gaggattcta aggatgtttt aggattgcta gttcctagat taaaatctat atctcaagaa
    15781 gttcatttag atgataacat aaaaaataat ataacaaaag attttaaatc tgagttctac
    15841 tttgatatga ttgatggcaa catatgttgt gatgtaaagt atatatatga tgatgaagat
    15901 aaaaaatttg tgctccccaa catacaaaag gaagctacta ttgaaaataa acttacttct
    15961 tcacaattta caaaggaaaa taatcattat gtgtttaaag gaactgacca tgacctattt
    16021 acatttttag atttacaatt agaatctcta aaggaatttg gagatatata ttattctgag
    16081 aagtttaaat tgaaaaaaat atataacgct tcatcaattc aagcttcaat caatcagact
    16141 aaccagaatt acctagaatt taattttaat atatctgata taaatccaaa agagtacaag
    16201 gatattttga aagcttttca agaaaaacgt actttttata aacttaaaga tggaaacttt
    16261 atagatttat cagaaagaga aacaaaagat ttttttgaat tagttgaaaa tctagatata
    16321 atggataaat caaaaaataa taaaattcat aaaaataaag ctctatatat aaatgatgta
    16381 atacgtagta aaaatttaaa gtttatagat ggtaaaaaag aactagacag tatatgtgaa
    16441 aaatttagaa attttgatag cataaattta gaaattcccg caaatttaaa tgcaaacctt
    16501 cgtgactatc aaataaatgg cctcaattgg tttaaagttt tagattatta taaatttggt
    16561 ggtatattag ctgatgaaat gggtcttggt aaaacaatac agactattgc atttttatta
    16621 tcattatcta ataaaaaaag tcttattgtt acaccgacat cactcattta taattggaaa
    16681 aatgaatttg aaaagtttgc tccagatata aaagttttat tgatacatgg aaataaaaga
    16741 gatagagaaa aatgttttat ggaattagaa aattttgatg taatacttac aacatatggt
    16801 actcttagaa atgatttaga caaatatagt gaaataaagt ttgattattg tatattagac
    16861 gaagctcaaa atataaaaaa tccagtggct ttagtaactg agtctgtaaa aagtataaat
    16921 gcagaaaata aatttgcact cactggtaca cctatggaaa ataatctact agaattatgg
    16981 tctatatttg attttataat gcctggctat ctatatagta aagcaaagtt tcaggaattg
    17041 tttataaata aagaagataa tgttaaaaac ctgaaaaaat tgataaaacc ttttattctt
    17101 agaagaagta aaaaacaagt tatgaaagaa cttccagata agattgaaaa aaactttttt
    17161 gttgaattga ataaagagca gaaaaagata tatagtgtat attcaaaaga tatacaagat
    17221 aaaatgaaag ataaaaatct aaagaaagat aaaatagtta tattctctta tttaactaaa
    17281 ctaagacaac tttgtttaga cccaagtata gtagtaaaag attacaataa aaaaagttca
    17341 aaaattgaaa catgtttaga aatacttagg gatagtataa acgaaaacca caaaatatta
    17401 ttgttttcac aatttacttc tgtgcttaag aacataagta aggaacttga taagtataaa
    17461 attaaatacc attatataga tggtaaaact aatgccaaag aaagacttga acttgtagat
    17521 gagttcaata atagtatgga taaaaaagtt tttttaatat ctttaaaagc aggcggaact
    17581 ggtttaaatc ttacatctgc tgacatggta attcactttg acccatggtg gaatccatca
    17641 gtagaaaatc aagctagtga tagagctcat agatttggtc aaaaaaattc agtacaagtt
    17701 ataaaattga ttgctaaagg gactatagaa gaaaaaataa taaaattaca agaaagtaaa
    17761 aaagagctta taaatcaatt tataaatgga gaactttcta atgaaggtgt attaaaaagt
    17821 ctatctgatg aggagataat aaacttattt aactaaatta tttaatccta cagcttaact
    17881 atatgaattt gttctaacat ttatatagtt aagctaaaat atctgaataa ataaaattta
    17941 atattgttat ttaatcccaa ctttacaatc taatgatatc tctttctata catattgcat
    18001 atacaaatgt aataacttga ctattagaat attttgtttt aacaatccta tttcatcata
    18061 ataatgaagt atatgtacac tcacttttgt aagttttgca atccatttta cagttttcat
    18121 tattgtaaat tttggttaaa ttattcctct aaaaatttct ttgctacatt tgaaaaatct
    18181 aatccattcg ataaaattgc aggatgacca cctttatcaa acaaatacat tttaccattt
    18241 ttaatctttt caagaagtaa tgaaaaagta ttttcaaaaa aatcactaga tattagagat
    18301 acaaactcat cttctctact tcctgttaaa agcacctctg gttgcatagt ttctagaggc
    18361 ttatgaaaaa actttcctat agttttataa tgttcaaaaa tagcttctgt atcattatct
    18421 acaacttttt cccagttttc tccttgatta taaatataaa aagcttttgc accatcatct
    18481 tgcttagatg cttcacgctc tattttcaca ttttgtacaa atgactctag aggaacttct
    18541 ccttcaaaac tatctgcaat taccttattc actaaatcag gtctctcaag tgcaacattt
    18601 aatgcaacaa gagctcctcc actactacca attatgttta cttttttata gttcattgct
    18661 tctaaaaaac atatcacttg catagcctca tcaaaccata agtcagcaga aaatttatcg
    18721 accatttgag attttccatg acctacaaaa tcaattaata ttactttata ttcatcttta
    18781 taaaaattga ttatatcatt aaacatcttg gaagaggctg tatttccatg taaaaaaagt
    18841 aatggtgtac catccccaat ttcattatag tagcactgtt tgtcgttata gttaaaatat
    18901 gccatatatt tccccccttt aatatcccta taggtatata tttattactt atatttaaaa
    18961 tgactattcc cttagccact taaaaatagt ataacttaga atgtagataa aatcaacatt
    19021 ccttgctaat ttatctattg tttgtctaat ttattataat ttaacttctg taaaatttct
    19081 tttttgtacg aaaatattcc ttccatttac aacaatcatt caaatgaagt ataacatgac
    19141 gagtaggagg cataagcaaa attccagcaa tatctttatt cccccaaaag tccaacaacc
    19201 aaatagaatc ttcatgcttt attactcctc cagtcaccaa aatcctttct aagtcaattg
    19261 gagagacctt tcgtttcata tcttcagctt tcaaatcctt aacaacttct attgtgtttt
    19321 ttgcaacttc atttcgataa caaagcaatt catcaatatt gactaaatta tcgtatatct
    19381 cttttcactt taaccatttt tttctttata ggtatctcct tatatttttt taatatcaaa
    19441 tctcccttat aatttaatat atctacatat gaacataaat cttgaacttc aattacacct
    19501 atatcatcat tagttatacc ttttatatta ctaaatgcac caactatatc taaaactctt
    19561 attttcttct tcttaccagc atttaaatat aatttaacaa cttcactttc tatatttagt
    19621 tttatatctt tacttttgta agaattttta tcaacttttt ttctctttga tacttttttt
    19681 aggattcctt ttgaatattc ttcaaacaaa actttgccat gtgtaattct acttttttct
    19741 atatctttta attcatttat tttatatcct atatacgttt ctatattctc tatatatttt
    19801 tcatcctttt cagttactat agtaattgcc tttccactat ttccttttct cccagttcta
    19861 cctattctat gtatatagtt ttctttatct tgtggcacat cataatttat tactaaagaa
    19921 atatcatcaa tatgtattcc tctggacgct acatcactac taactaatat attaaatttt
    19981 tggtttttaa aatccttaat tacaaaaatt cttctttctt gtgataaatc tgcatgtaac
    20041 tctcttatta aaaaaccctc tttactcatc ttgttatata atttagatac tttctctttg
    20101 gtattacaaa atattatcac actctttgga ttttcgctgt atattaattc ttttaaaagg
    20161 atgtacttct ctcttccatt tgccttgatt attttgtcat ctatttgtct tgttttttga
    20221 ttagtatcgc tctcattttc ttctatattt ataacactgt aatctagcat atatttttca
    20281 catatatatt ttatctcttc atctattgta gctgaaaaaa gacttactat tttctcctta
    20341 ggtaaattta ataatatttt ttccatttgt tcaacaaaac ctttattaaa cattttatct
    20401 gcctcatcta ttactaaata ctttacattt tccaacttaa tagaacctct atttatatgg
    20461 tcaagtattc tgcctggagt agctacaaca atatgaaccc tttgtttcaa ttcagctatt
    20521 tggtctttta tagattgttt gccaaatata gcactacatc tcaccttctt tagtctccct
    20581 atatctgata tctcatcttt tacttgcaag gctaattctc ttgttggaac cactataaga
    20641 gcttgaatat tattgtaatc tacatttata ttctcgcata aaggtattgc aaaacttgct
    20701 gtctttccac ttcctgtttt tgacctaact actaagtttt gaccttttaa taattttgga
    20761 ataacttctc tttgtactct agaaggtata ttatatccta aactcttcaa tgacttaagt
    20821 atcttttcat ttaacttaaa tttttcaaat gtatatttca tatattacca ccttattata
    20881 tttatcttat ctatatattt ttctacatta tattattata atacaaggtt ctactatttt
    20941 tatattattt attagactga agataaaagt atatactcta taaacctata gtttagtagc
    21001 aaattaatta aatttttata tttaaatacc tccttaaata tactgataaa tcaatatttt
    21061 caaggaggta tctcttgtca ataatcaaac taatctatct tttgaaagtt taaattatac
    21121 taatacattg tatttttatt tatatctaat attaaaaggt attaatcaat acctaaatca
    21181 ccataatctc acatatttat atctttattc aatatacctt ctgtaatcaa tcgtgctata
    21241 taatcataac ctgattcatt tgctttgtta caatcattca cattatcaca gaaaaaactt
    21301 tcaatctgta ttgcaactgg atttgtattt ctcaatatat ataaattttc tcttttttta
    21361 gctcctctat tttcaaataa ttctccaagt ctattagata ctcttttagc atattctaat
    21421 ccttcagtgc catagtaaag cacttctgta ccacattgtg ttccatcagc tttatttaaa
    21481 tgtagctcta ctaataaatc gtatttacca ctgtttactt ttggaactct atagaaaaat
    21541 tcttctcttt ctgacataaa gtgcctttct ggacaaatta ttacatctac tgtattacct
    21601 tgacttataa gcatctcagc taacatagga gctaattttt tattataatt gtattcatca
    21661 actataccat ttacacctgt acttttacca ccttttagta tactatgacc tactgttatg
    21721 catattttca tttacttttt tcaccttctt ttaagccaat ttttatataa tagtaatata
    21781 attttaaagt tattactaat aataatatat taattgaaga aggtaaaagt tctccgtgat
    21841 tatcattttt ttaacatatt tgtcttattt tatcataaat ctatattttt taaatattat
    21901 actatactta ttatttataa ttgtttaatt aatcatttat atatatgata aatatatcaa
    21961 tatctatttt cctttaacca aatgctattt taataagttt aagttttagc tttaaaatac
    22021 ttaataatac aataaaagtg atgtatttac aaataattct tctggattta attcatttat
    22081 tgatgtatgt tcaattttta tatttatatt tttcatatct tcataaataa ttccaactgg
    22141 agaacaaatg aaacaagaaa atccaaaagt ttttgttatt ttttttacct tattatcacc
    22201 aaaacttatt tgctttttac aatcgtttat attttcattg ttattttgta tatcattttt
    22261 ataatactta tcttttttaa tgtcattaga cttatttact gttttaaaat tactcctatt
    22321 tttacaacta tagtctattt caacttctat atacccaaaa actatcaagt tacataattt
    22381 caaattacta cctttgttta taagattttt cataggttta acatcaaata ttttactatt
    22441 tacatttact tttaatatat ctcttagatt tttaggagct tttaaagtat tacttatatt
    22501 caattgtttg aatacaattt tatttacatt agtataaact ctattacttt ctattgatat
    22561 tcttgttggg aatttatcta gattacactt ataataatag tatatattag cactattctt
    22621 tataatccta tgtgcagaat ttggtaaaac tacactctta aaagatatta tttttttaca
    22681 acttgcatct attctcccta tgtaaaaacc ttgctgtggg tctaatccac gttttataat
    22741 gccattaact tttaccgtat tatttataaa ctctatatat ttagaaatat aatctttgaa
    22801 aaatatattt ccagttttgt acctacttgt atttgtaaca ataatagtgt aggtcaaaat
    22861 ctcaaataca gatgcacatt ttttatcaac atatttttct acttttataa tacttccatt
    22921 tgcatcattt gaaaatacat cattaaaagt agttttcgtt attctcattg catccacctt
    22981 tctataattt aaagattact ctatcttgga actattttca taacttataa ttgcagtaac
    23041 actaataaat aaattatcat ctaaaataga tgaagtaata tcttgtataa gatactttaa
    23101 attaatttta tctttatttt ctatctttct aggcatttgt ataaatgtac taaagggtat
    23161 ttttaattta aatgtatttt ttttactaga atttctattt cttttattgc ctgcataatc
    23221 taatattgca tcaatatcta tatcaccaac taaaatcaac ttctttcctg taagttgttc
    23281 atcttctaat gatatacatt ctatagtatc aacaagactt atattgttaa tattcatatt
    23341 gtaaacatat gtctctaatg aatttattac agttttttta gaagctaatt taaaattttt
    23401 atatgtatta aattgagata aataaagagt cttttctagt ttttcacatc tttcaaggtt
    23461 gtatattacc ttattatttt catacataat cgcatcaatc tccctgtatc tatatattaa
    23521 tactattcat aaaaattatt tcaaattacc attttttatt attcactatc tagttgttat
    23581 ttacttttaa gaaagaatcc ttttttactt cattgatttc aatttacaag taaaagtctt
    23641 tctgaaaaaa aaatatatat atctctattc acttttttat aagtaattat aatttttata
    23701 gactaacggt cagccttaag ggaaaaggtg ttctttaagt gcagaattta gataaagtaa
    23761 ataatccttt tataaataat tccaagtttt aacttaaata atttgattta cagtataaag
    23821 taaattttga aagataatga tatcgattta ataattacaa gtaaaagcat gtcaaaataa
    23881 ggctatgatt ttaaaaatca tagccttatc aataaaaatt taatcaaagt tacatttcac
    23941 tttgaatttc actgtctatt aattgaagat tatactgttg aattaattct attagacgag
    24001 ctgcatatat actattgcca ttttcatcct tcgcagtact ataacctgcc ttttctaatg
    24061 ctttagcttg atatatatac gtttttgcat caaatacacc attgttttta taccttttat
    24121 ttgtggctaa aaattttgca tggtctttta tagattcatt tttatcatca tatattctaa
    24181 atttatcatc tataattgta tctaaatgtt ctttcgtttc taaagtcact gacttgcctt
    24241 tccaataagc gtcagccttt ataccaaata gattgttata ttgttttgcc aaagttgact
    24301 tgccccagct agattctatt atagcttgtg caatagttat agatggaagt attttataat
    24361 ccttataatt ttgtattgct tctgttttaa tttcatcaat aaacttcata tattttgtat
    24421 cagacttcaa tctttctggt tttataccat aatccttaag attgtctata taattatcaa
    24481 ccaatctttt ttgtctatta ctaaattcta actcatccaa aatatcactt aacttattta
    24541 ttttattatt tttagaaaaa ttcttaacaa ataaatctga tacttcttgt atctgtgaat
    24601 ccttaacgtt ttttaagtta tttttattta acacagcaac aatggaagct acatacttcc
    24661 agttaacttg tgcattattt ttacttgcta aatcagcata ttttatatac ttactgacat
    24721 ttatatcatc cttttttatc tctctagatg aatattcctt cactaataca cctattaaaa
    24781 ttgatattat cagtattata acaatgaatt ttttattctt cataaatatt gtcctccctt
    24841 atactatctg ctgaatataa tataccttac ttttgtgaca tattctttac gaaattttga
    24901 aaattttatg aagatttatg aaatatattt aattaaaagc actactattg ttataatata
    24961 tagttttcta tcacacaaaa tagttaaaat aaataaataa ttagatagct tattttaact
    25021 atttatataa taaaaatatt attaaatcta tttatcaatt tttatacatt tttctaatat
    25081 atattttttc ccatatacaa taatttttat tggtttacct tttaaatttt ctattttaat
    25141 taaatcacta catacatcta cattaataat attatctcta taatttatat tgaaagaata
    25201 atttttccac tcttttggca acttagggtc aaaagataga gtttcattcc atgttctcat
    25261 gcctgcaaat ccatgtacaa tggaaagcca tgaaccagac attgaagtta tgtgcagtcc
    25321 atcatttgta tcattattgt aattatctaa atcaagccta gctgttcttg aatataattc
    25381 ataagccttt tctaggttat ctatttctga ggcaataata gaataaatac tagaagataa
    25441 agaagattca tgtacagtat atttttcata gaagtcaaaa tttctctttt tttcttcttt
    25501 agtaaatcta tttccaaaat agtaaattcc ctgtaataca tctgcttgtt ttataaaaca
    25561 cgacctaaga atcttatccc aagaccaatt ttgattaaga ggaaaatttt cttttggtaa
    25621 atcctttact tctacaaatt ctttatctaa aaaattatca ttttgaatta ttatttgtag
    25681 ttcctcatca tacagcagat acattttatt tgatattatt ttccaaaact caacttcttc
    25741 cctagtcaca ttatttctgt caatacattc attacactct ttctctaatc gtaagatatt
    25801 ttctacagca taatctaaac accatttagc tatataatta gtataccaat tattattgat
    25861 attgttttca tactcgtttg gacctgttac tccatgtatc atatacaaat ctttttttgt
    25921 agacaaatga actctacttg cccaaaatct agctatttct attattacat ctattccttt
    25981 ttcttttata tatttatagt cacctgtata atttgtatag ttatatattg catatactat
    26041 acttccattt cggtggattt cctcaaatgt tatctcccat tcattatgac attctactcc
    26101 atcaaatgtt accattggat ataaagcacc tttaagacct agtttttttg cattatcctt
    26161 tgcctgttct aactgattgt acctatattc taaaagattt ctagttacct ccttatctgc
    26221 tacacctaga tatactggaa cacaaaacgc ttctgtatcc caataagtag ctcctccata
    26281 cttttcacca gtaaatcctt ttggacctat atttaatcgc gaatcttctc cataatatgt
    26341 tgaaaatagt tggaataaat tatatcttat accttgttgg ctacttttat ctccatttat
    26401 ttttacatct gcactttccc atcttttatt ccataacttt atatgttcat ttaatatatc
    26461 atcataactt ttagatagtt ctctatttaa aatgtcttca cattttctta atagctcact
    26521 ttccttataa tctctagttg aggttataga tacatatttt tctatacttg caacctcatt
    26581 ttttaaaaca tccattgtta tcgaattttc acagtaatta taatcactct catgagatac
    26641 agcttctaca tcaccatttg tcttaatata catcattgaa cccacagtga atactggtgt
    26701 attaaatgca ttctcctttg ttcgagaaag aatactccct ttatattcta aagaacttga
    26761 agaaattcca ttccaaaact tttcctcata gtttgaatct tcatttttta tatttgaatc
    26821 taaatatggt acaaatttta ttttagtatt agaatttaat gattttatac tgtatttaat
    26881 tactgcaagt tccttaacag atatacttaa aaacctagta accctaactt caaatttttt
    26941 atcatttata tttacaataa acctacgatt taaaatacca tttttcatat ctaattctct
    27001 atgaaaatct tctactttac attttgctaa atctaattct acatcatcta tgaaaattct
    27061 tacacctata tagttgactg aattaggaat tttaccaaag tattctggat acccattttt
    27121 ccaccaacca acacgagttt tatctggaaa ccatactcca gctatataag accccttgtg
    27181 tgtatctcca ctataatttt cctcatagtt tcctctcata cccatatatc cattgcctat
    27241 actcattatg gactctgcta atctattttc tttgatttca atcttatctt gtataatctt
    27301 ccactcatct acattactaa ataaacattt catataattc tcctaactat atactcatta
    27361 tacttttctt gtatatattc aaactttaat tgatttgttg aatctacaac taaatttgct
    27421 ttttttaagt tttcataatt tccaacacca acagaaaaca tattagctga gtttatagcg
    27481 tctatacctg cacttgcatc ctctatccct atgcaatttt gtggatttac attaagccct
    27541 ttcgctgaca taagaaatat ctctggatgt ggtttattgt ttttacattt tcctgcatca
    27601 gctataaaat caaatttgtc acttataccc aaatgattta atacatttat tgcattctta
    27661 cttgcagaag atagacctat ctttatatta ttagatttta catcaattaa caagctttct
    27721 atgcctggca aaatatcatt tgaagttatc tcatctatca atgatacata gtaattattt
    27781 ttttcttcag ccattctcac tttttcttct tctgaaaatg aatatttctt atttccaaat
    27841 tctaatatcc tgtccaatga ctccattcta cttatgcctt ttaaagattc attaaatttt
    27901 gtgtctatat ctatcccaac tttatgagct agttttctcc aagccatata gtgataatat
    27961 gcagtatctg taattacacc atctaaatca aatataaatg cttcaatcat tcttatttcc
    28021 tctttctaca agaaattttt agactaataa tcaatttatt ttctatcttg tatttaagat
    28081 attatttata aaattatcta ctctaactca gtctttaatt taaaatctac tttcaattca
    28141 ctcacttgta gaccatattt tttatcgttt ataaagactt gtttaaactg tataataatt
    28201 taacatgaaa tctaaaataa atactttttt gtattatttt aaaataatga acttgtattt
    28261 ctctaacatt agttcttctt ttaaatcaac tatttcttct tcatataaat cttttacttt
    28321 tttatttgct aaataatctg gtaacgtcaa aattatattt ttagatgaat tatttattat
    28381 tatagaaata gattcttttt tcaatataat cgttccattt tccttatctg ctaacaccca
    28441 ttcatttgat atagacctaa gttctttatt ttctttacga agtctaataa tcttcttcat
    28501 aaaatcaaat atatccttat tccatttttc ttcattccat tccatacatt ttctttgacc
    28561 ttcagatatt gaacttaatt ctccttccat tccgatttca tcaccatagt atatacaagg
    28621 acatcctgtt tgtacaaaca taaacatata tgcaagttta aatctatcaa tatttccatt
    28681 agcataagtc aatattcttg tagtatcgtg acttccaagt aaattaaaaa taacttcact
    28741 aacttgtatt ggataactta ccattacatc atttatcata tacttaaatt cttcggcgtt
    28801 tatctcatct gtacaaaaga attttttcat ggcttctgac atcaagtaat tcataactga
    28861 atcaaattgg tcacccatta accatggtaa actaccatgc caaatttctc ccaatatata
    28921 aatgtctggt tttacttttt tgacttcttc tctaaacttt ctccaaaaaa catggtctac
    28981 ttcatttgaa acatctaatc tccacgcatc tatatcaaat tcctgtatcc agtacttgcc
    29041 tacatccaat aaatatttta ttacttctga attttctgta ttaagtttag gcatatattt
    29101 ttcacaccca aaggtttcat aaggtatatt tttttcatct atttgttcta ttggagtatc
    29161 aattttactc atatctttta tataaaacca gtttttatat cgagaatttt ccttattttg
    29221 aactacatct tgccactgtt tagaataata tcctatatga ttaaaaacag catccaacat
    29281 tatttttata tttctcttat gcgcttcttc tattagtttt tttaaagtct ctttatctcc
    29341 taatgtaggg tctacctcca tataatctac cgtatcatat ctatgattgg tttttccaat
    29401 tgtaataggg caaaagtaaa ttccgtttat tcccaaatca ctcaaataat ctaagtgttc
    29461 tatcacacct tgtaaatctc cacctgtaaa attatctctg gttggaatag ctccccattt
    29521 atctactcct tctgggttta tagacgggtc accatttgca aatctatctg gaaatatctg
    29581 ataccaaaca gtatctttca cccaatttgg cactttaggt atatctatat aatttaaata
    29641 tggatatcca aaaaaattac ctatttcaca taattccttt tcatcatttt catttcccaa
    29701 ctctattatt cttttttctg taaataaaag tttttcctct tgaccttcta ttataaagcc
    29761 atatcgtgag cgtttattta aaggcttaca cttagctatc caatagtcaa ataattcagt
    29821 ttctacttct ttactcatta ctatatttgt tccaccagac caaccaactc cagatgcatt
    29881 taaatttcct cctcctgcac ctcctttatc ccacacatat tggtctccta ttcgtaaaat
    29941 tactcttttc gcttcatttt ttttagttct aagtcttata tgtagtgttt ctctatcata
    30001 accataggca taattgctca ttggtatatg taatatagct tcttttgtta aactattttg
    30061 aattgttcct ttgatttgat tacttaaagt tatattttct ttatttttat tagacatttt
    30121 aaaatttccc tcctttataa taatttatat aatctaaaga taggttaata tattttacta
    30181 ttatatcaca ataaaaatat ataatagtaa aatatatcaa caataaacta ttcaatttaa
    30241 aataagctat actatttaaa tttaattcta tttagttatt ttagcaaaat aaaaacctct
    30301 agtatttttc tagaggatac aatactataa ctattaactt tttaactatc tattaattta
    30361 cttcaaaaca cactctaagc tatattgtta gatttaactt tttctatatt tacaaaatag
    30421 gtttctcttt ctttaaataa agatacatat ttaaagtcaa gatacttaag catatcatag
    30481 gccacatcaa aatctttgca tatatcttct accctatgag aatcagaacc tacagtaact
    30541 ataaatcctc caagttcttt atatcttttt agaatatctt catttggatg tatagcattt
    30601 agtccatatc tataaccaga agtatttagc tctataccac tcccattatt gattattgac
    30661 tttaagacct catcaataac atctttaaag tcattgtata taactctatt atctgaatat
    30721 attccatacc tagatatata atccaaatga ccatatatat tgtaatctcc aaattcacga
    30781 ttagtttcaa gtatgctatt aaaatattta taatacgcat cttctttgct aagaccttta
    30841 aaatacgcct ttgatggaac aggtacattg tcaattgtat gagttgagca caatacaaaa
    30901 tcaaaaggat tagaatttat tatttcttct atttcatttt ttagatgttt tctgtaaccc
    30961 acttcaacac ctagtaataa ctctatgtcc tttttatatt tttctttaaa cttattaaat
    31021 actggaacat agtcattata atttatgcaa aatcctctat cttcatcatg ttctaaatgg
    31081 tctgtaaaag tcaaatatct aacaccaata cttatagctt tttttattgt atcttccatg
    31141 gaatcctttc catcacttga aaaattagta tgaatatgat tatctattaa attcatatta
    31201 tccctccctt tattttatct atcttaatta tacaacaagt ttattgtata catttgttaa
    31261 aagaaaatta taatggttaa attttattta catactttta tttttacaaa aaagagaagc
    31321 acctatttct gattttagaa aatcattaag gatacttctc ctaatgttac ttttatatac
    31381 ttcaatttaa tttttaaaag tcttaatttt gtaatagagt ttagctgtta tatgtccaat
    31441 catataaaaa gctgtataaa gtaaaacata tatatatgca gaatcattta aaaatatata
    31501 cattaatact atataagtta ctaaagaaac aataagagat aaaatccaat tagtcttatt
    31561 taatacacaa attatccctt gtatcaaaaa agaaattgga aaaatcaaaa ccattcccac
    31621 aacaaaaata cttttagatt gaatatctgg aatattaaat atatctgtag caaaagttaa
    31681 aaacattagc accattatga ctgtaggaaa tatgatacta aaaatattac tctttttcat
    31741 aataatcaat ccttatttat tggatatttg ataatcttca attattttgt tgcctcaatt
    31801 tctcccatca ttcttactaa atcttgtcca tattgacctc cagcccaatt attagataaa
    31861 ctttcaactg ttttagcctt accaaataaa gatttttcat gtctagggtc tactggattt
    31921 gaaagtgggt aaccatcttg tcctgcatat aagcataaat gttgaatttg agctaatatt
    31981 ccttcttccc aagtatcaaa tcttgaatgg gcattcttat cagtatcaga accacctaca
    32041 gaagttttaa gtccacaagg attcttaaat gatgcatcta atactccacc aaaattacaa
    32101 aaaccagttt cttttgcaga ttgagctact gccaaaacag gattaacacc ttcctgaaca
    32161 gcagtatcat ataatattgg tattaactct ataaataaat cattagcttt tttagatttt
    32221 gcccatttta agcacgcttc taaactagct gttggctgac ctaatatgct tgtctcattt
    32281 ttcataggtg actcaccttt tgtagacgaa tcgccattat tttgtgcagc agaaacacac
    32341 ttattataca cactgtcact tattgaacct aatctagtaa tcttatctgc tcctgctaat
    32401 atagacttat cactactttc tgacaccaat acaattggat tttccttagc aattgtagaa
    32461 cctgtaagtg catttactaa atcatatcca tcactcaaat aaaattctga tgcatctgga
    32521 taaaactctt ttataacttt cttatttgta tcatatctat ctgaaccacc aagtctttta
    32581 gcattagttt tattaactaa actagtgctc attgatatac tcccacctac tgcatatgtt
    32641 tttacattgc ttgttgaaaa aggcacactc tttccatctg taagtactat aggagctata
    32701 tctcttactg atacgggtgc agcacttatt gtatctgctt ctcctttata tgcattagtt
    32761 aatatcactt catcaacttt tttgatacca tttacctctt tagcaacact ataactagtc
    32821 tttattctgt catctccacc caatctagtt acctgtgccc ctgtgttttt tagttcagtt
    32881 tctactgact tacttattga taattcttta cctattatgt atatcttttt aacatttttt
    32941 aatctttgtt ttgtttcatt tggtatctta tttttttgtg caagcaaaat tggagcatca
    33001 acagcacctg caagaccact tgctgaaaga ccatctgcta tagaattatc catatttacc
    33061 ataattgctg tatcataact ctttttatct gctattaatc cagccgtctc atatatattg
    33121 ttacctttaa ttgtatctga tgttaatgca tctacattag gtacatttat tagaaatata
    33181 gctaatgtta aacctaatgc taaaactctt ctaattgttc tcatattctt cccccttgat
    33241 atatcaagtt taattcaaaa atgactataa catagtatta catctacgct atagtcattt
    33301 attataattt aataattata tatttgttat tatatcataa attacaaaaa gtttccataa
    33361 tattgtatag attaaaggaa aaatttacat tttagttaca atttactaga tttttgttct
    33421 aatgtaccta tatttagtaa caatttgtaa taaaaatcga tatctatatt acatatttaa
    33481 ttcactgttt tacaaaatga aattttattg tatacaataa atagattata tgtttacata
    33541 atctatatta tttttaagta aatcttttat cgatattatt aagaatatat attacatcaa
    33601 gtttattacg tgcaaaccac tatcaactta aatacctaag gtaacacttg aagtagtaga
    33661 ttcttggtaa caataattat tgctatcatt atcttttttt aattaagtat ttactattta
    33721 tatatcaatc tacttttcac ttttaattta attctaatat ttttttatat gttttatgat
    33781 atataatgtg atatattcta attaatgata ttttcaagta aaaaaatgtt actctattat
    33841 aaggtgatag tatacttgaa aataaataaa acacttaact ttatttaagg agatttatga
    33901 tgagtaacaa acaaggttct aacatcaaag atgtaaaaat taaaaataag ttaataatat
    33961 tgaaattact ttctacaaat acacctatgt ccagagttga tttagctaag tctacaggac
    34021 ttactaaaat gactttatca aatctagtta ctgaacttat taatgaaaat atgattaggg
    34081 aaatagagtc atcaacttta aatgcacatt catcaggtcg taaacctatt ttattagata
    34141 tttctaatat atcgccttgt atttgtggaa tcctaataag aagaaatata tgtaaggtaa
    34201 ttatttcaga tttaaaaggt aatattgtaa aacaactttc tgaaacttac cctaaatttc
    34261 tatcaaatga tgttttaaaa gatataataa aaagaatgtt tactaaaata atgaaaatca
    34321 acaagagaga tataattgga attggtattt cttcaatagg tccagtagat gatataaatg
    34381 gtaccatact aaatccccca gattttggaa atgtatcaaa cctaaatata gtagatttta
    34441 taaaagaaat gtctgatttg ccaatatttt tgataaatga tgctaattcg ggtgcattag
    34501 cagaaaaaat gtatggttta ggtaaaaata tatcaaatta tatataccta cacataatga
    34561 atggtattgg agctggtcta gttctagaaa ataaacttca tactggcaac ctaggacaaa
    34621 gtggagaaat aggacataca tcaattaatt ttagtggacc attgtgcagt tgtggtaaca
    34681 atggatgttt agattattat acaagtgtca gtaatctaat taaaagaatt gaatcattga
    34741 gccatattta tcctaactcc ccattaatta actgtaaaga tttttcttta gtcaagttaa
    34801 tagatgaggc aaataataaa gatagtcttg ccatgttttt gcttgatgaa ttttgtacat
    34861 acgtatcata tgcattagtt aatacactta cattgattga ttgtagttct ataataatag
    34921 gctatgactt taatatacct ggtacattta tagaaaatac attattaaaa aaattgactg
    34981 cttcagcaag tttttctaaa tataaaaaaa taagtgtaag gcattcaaat tttggagcaa
    35041 atgcaccatt aattggctct atagccattg taagttataa tttttttaat ggtagtataa
    35101 atttttatta aatatgtaaa atatttaaac acttttattg aatatttttc catttaatat
    35161 tattatattt tataagagca tttaaaattt ggaggtataa aaatgatttc agctaaaatg
    35221 gaaaaattac ttaatgagca gattaaccat gaattatatt ctgcgtatct ttatctttct
    35281 atgtcttctt accttgaagc agaaggactt aaaggatttt ctaattggtt ttatgttcaa
    35341 tataaagaag agacagacca cgctatgttt ttttacaaat acttacataa tgtaggagga
    35401 aaggttcagc ttgatgctat cccaatgcca gattcagact ttacttctgc aatggatatt
    35461 cttgagcgta cgttagctca tgaaaaaaag gttacagctt taataaacaa cttagcagct
    35521 gtagcaaact ctgaatcaga ctttagaact tctcaattct tactttggtt tatatctgaa
    35581 caagcagaag aagaaacaaa ttgtgaagac aatataaaac gtgttaaact tgctggagag
    35641 ggtgggctat tctttgttga ccaagaattt gctaatcgta catatgcagc tccaacaaac
    35701 ccacctgtga caatataacg tttaaaaatt tttaatataa taaagaaggt gttttaatga
    35761 aaatcaagat tgattcttat cattaaaaca ccttttatta ttcgttttat acgtagtatt
    35821 ttattgttta tacagtgcta tttcacaatg aaacttaaaa ctatactttc attttaaaat
    35881 atttttaaat taaatttggg gtgactgaaa atcacactat ctaacttaca aaatgtgatt
    35941 ttcagttggt gctttaatta tggttgaata ccaaaactta taaactattt atatttaact
    36001 attaaggcat ctactgcaca tacgcctttt cctttaggat atacaaacac tggatttaag
    36061 tctatttcct tgacttcatc cttattttca taagcatatt ttcctaactt gaccatcata
    36121 tctgcaagag catcaatatc acatggctca cttcctctat agccatttaa aagcttaaat
    36181 gattttagtt ttttaagcat cataatagct tcatcatgat ttataggtaa tggatataat
    36241 gtagtatctt taaatacctc tacaaatact cctcctaaac cgactaataa cattggtcca
    36301 aattggtcat cattagttat accaataata atttctatac cactttctac catttcttga
    36361 actaatatac catcaatatt agcatctggt tttgcttttt ttacattagt taaaatctca
    36421 ttatatgcat ctactgcctc atctctattc ttaattccta ttttaactcc tccaacatca
    36481 gatttgtgca atatttcaga agaatttatt tttaaaacaa caggatactt aatatatttt
    36541 aagatttcat ctaattcatc aatacttttt gcattacctt gtcctggaac tggtactcca
    36601 tattttttca tctcaatctt actatctaat tctgataatg ctactacatt atttgactct
    36661 cttttcttaa cagctttaaa ttctaaagtt ctttttgaat aatcataatc tataaactta
    36721 gccattttat taaaacaagt aaatgtagtt ccaactgatg acattatagg cacttgatta
    36781 tcttctaatc tagtcctata atctaaatat ctatttcctt caagtgtagg aactgcaaac
    36841 acaggttttt tcaatccttg ttctctagct tcacttacag ctttacaaag ggtttcatgc
    36901 atttcatttt cttcatctct tatatttgca cctaccatag taaatccaac actatcatcc
    36961 ttttcaaagg tatgtaataa acctactatc atatcctttt catggaataa tgctgttgta
    37021 gcatctagtg gatttttagg tgttgcaaat cctggaagat atttttccat ttcttcttta
    37081 gtttcagaag atatttctgc aagttctaca ccattttctt ctgctaaatc tgcacaaata
    37141 gtattttctc ctcctgagaa gtttattgct gcaaaattag aatttgcagg taaattacca
    37201 tctaaaacac taaaagcttg tgctaaacac ataaactgtt ctaaattttc cacagaaatt
    37261 actccatatt tttcaaaaat actttcaaaa gctttactcg aaccagctaa atttcctgta
    37321 tgagaagcag cagaaatagc acctttttct gaccttcctg atttcaatat tattactggc
    37381 tttctttttt gtgctgccct ttttaaagca tctataaatt tttgaggatt ttttaaacct
    37441 tcaaggtaaa ttgctattac tgatgcataa tcatcatcta caaggaaatc tactgcatct
    37501 tctagtgtaa ctatatttcc atttcctgtt gaaaaaccat aagatatatt aaaataatca
    37561 cttgaaacaa tttcagcagt tataaatcca ctttgaccta caattcctat accaggtttt
    37621 ttagtatcta aatcccattt agaacctcct gcccatagat taaccttatc tatacaattt
    37681 agcaacccca tgcagtttgg acccaatatt ttcatatcgt atgtatttga tatttctata
    37741 agttgatttt ctaaatctgt accttcctct gtaccttcct ctgcaaatcc actagcatat
    37801 accactgctg cctttgttcc taactctcca gaatctttta atacagatgg aactacattt
    37861 ctaggcgtac atactactac acagtctact acttctggaa cttcttgtat cgtcttataa
    37921 cattttctcc caaacaattc ttctcttttg ggattaacat aatatactct atctgtttcc
    37981 ttacttttta ctatactaag tgctgcactt cttccaaaac caagtttatc agtcactcct
    38041 actatagcaa tactttttgg tctaataagt ttttccaaac tcataaattc acgctcctat
    38101 atgtttatag ttgtgatttt aagtgaattt tatctcaaga tgaagtttct cttggcacta
    38161 aagattatta aatcttgaga taaatcactt aagataaggg taatttatca aatggcgtta
    38221 ttatagcctt ccatgaatgc tgcttcattt tgaacattaa actttccttt tcctttttcc
    38281 tcaaagaaat cacacatagt ttttaaagct aattcttttt caaataaccc agttttagca
    38341 attaatgctc caagtactac tatatttgct gcttttggat tgtttacttt ttgagctaat
    38401 tcctgacaat ttattttaac tacacttata tcttctctta atgttacatt ctcatctatt
    38461 gcagatgtat taataactac tgttccacct tctttaacta aatttatata atctaaagat
    38521 ggtttattca ttgctactaa aacatctatt tcttccatat caggcccacc tatactctca
    38581 tcagaaaact ttactacact ataggctttt ccacctctca tagttggacc atatgcaggc
    38641 atccaagtta cttgtttccc caattcaagt gctgactcag ctacaatcag accaagagtt
    38701 aaaactcctt gaccaccaaa ccctgcaaat actagctctt tttccataac tattcaccat
    38761 ccctttgctt aaattcgcca agagcataat atggcactat ttcttcttcc atccatttga
    38821 tagatttctc tagtgataca ccccagttag ttgggcaagg tgctaatatt tcaactaagg
    38881 aatatccctc tccatttaat tgagcttcta ttgcattttt tatatatttt tttagattaa
    38941 ttatttcctt tgggctatga actgaacctc ttgctacata tgctacatca aagaagtttg
    39001 ccaccatctc aggaactttc agtggactac cagtagtctt tatatctctt ccatttacac
    39061 ttgttgttgt gacttgaccc ggcatagtgg tccatgacat ttgtccacca gtcattgcaa
    39121 aattattatt gtttattaca aatacagtca cattatgatt tctatatgct gtatttaaag
    39181 tttcagaaag acctataaca taagcatctc catcaccttg gtaagtcatt actaatgtat
    39241 ctggaagtgc tactttaact cctatagctg tagatgaagc tctaccatgt gcagtttgca
    39301 tcttatcacc attccagcta aaattcatat tgcagacaca accaactcct aatgttaata
    39361 catgattttt ttcatatcca tgttcttcta ttacctctgc tattatacga tttactattc
    39421 catgaccaca acctgggcaa aacttgtttg gtattgaaac aacgctaggt gttaattttt
    39481 ctgtagccat attagaacac ctccttgatt tcgccagatt ttattttgct ataagtgtcc
    39541 attacttgca atgtttttgg tatatgattt ccactaaata aaccatacac aggtacattt
    39601 tctttacata ctttctttga ggctaaagca acatcttcta ctaattgacc tgtgtctgtt
    39661 gattctactg agataaatcc ttttacatct ttacttacct tttcaaatgc tttatatggg
    39721 aaaggccata ctgttattgg tcttattatt ccaacttttt caccttgttc tctaagtctt
    39781 ctaacagcat tcatagttgt tcttgatggt ataccaaacg ctacaaacac atattctgca
    39841 tcttctactt gataatcttc ccattgttgt tcattttcag ttattctcat acgtttctcc
    39901 atacaaaaag ttggcttttg gttatctgca acctttgcat ggtctctatt acttccatca
    39961 taagtccaac ctaaatctcc ttctctcttc ttaaactctg gaaattctac tggttccatc
    40021 atttgaccta aagctgcttc tgtcaaaatt tcgacaggca ctctgtactt ctccgctaca
    40081 tcaaatgagt tatacatcaa atctacagtc tcttgaatac tgtttggtgc ataaactatc
    40141 aaatgataat ctccatttcc tccccctcta gtatctctta gatagtctgt ctgtgctgag
    40201 tccaatgagc caagaccagg cccccatctc tgtacattta cgattacaca agggtaatga
    40261 tttgcacata agtaagaaat cccttcttgt tttaaagata ttcctggtcc tgaagaactc
    40321 gtcatagacc tcattccact tgcatatgct ccatatacca tatatatagc agccatctca
    40381 ttttcggact gtataaaagt tcttccaagt tcagacattc tatgtgataa aaattccatt
    40441 acctctgttt gtggtgtaat tggatatcct gcataaaatt cacaacctcc tcttagggct
    40501 gcttctgcaa tggcgtggtt ccccttccac atctttttca tattatccca actccttcta
    40561 agcctcttga catgcttctt ctttaattat ttcaaaaaca ttatctggac acacctgata
    40621 gcaaattcca caaagtatgc atttactctc atcaacttct acatgagcat aaccttcttt
    40681 atttatctta cttgatattt ttaatgctcc ttttttacaa gatataacgc agtattcaca
    40741 acttttacat ctctctgcgt ttatttttaa gttcatatct acaccccgtt tcataatatt
    40801 tctatctatt atccaatact tggctctcct ttaatattta atatttcctt taaaactgct
    40861 acaaatttcc tattttcttc catagttcca atacttacac gtgcatgtgt agcacttggt
    40921 cttattacaa accctctttt tttcaagtct tcaaataact taggtatatc tgttttcata
    40981 tctacataaa ggaaatttga ttgtgaatct acaacatcaa atcccatttt tgtaaactct
    41041 tctttaaagt aagccattcc aactctattt aattcatatg tttcttttat aaactcttca
    41101 tcatcaagtg ctgctgttgc tccacttata gcaacacgat ttgcagagaa aggctctttt
    41161 actgtaaata aagttcttat tatctcatct ttagcaatac catatcctac tctaagccct
    41221 gcaagaccat atattttaga gaaagttctt gttactataa cgttataatt ttcccttaca
    41281 tacttaagtc catccacata attaggaact gttaaaaatt ctatatatgc ttcatccaat
    41341 atagttataa tattttcagg gactttcttt aaaaattctt ctaactcttt atcatccaca
    41401 attgttcctg ttggattatt agggttacat acacatatta attttgtttt atctgtaatc
    41461 ttatctaata taccttgtaa atcatactta taatcttctg ttaaaggtac ttctactggt
    41521 atcccttcat tttttatagt tgcagttcta tatgatggaa atgttgggtt acaatatata
    41581 acttcatctc ctctatttat aaaagcctca cctattagag tgataacgtt atctgcacca
    41641 ttagcaacaa taaaattttc tggttttaaa tctagctttt cagctaattt tcttctaagt
    41701 tcgttagctt ctggctctgg ataaagttga ccttgtttta acatttcaat catagcttct
    41761 attgcttttg gtgatggtcc tagaggattt tcatttgaag ctaattttat tatttctttt
    41821 acaccatatt tttccttaac tttttctatt ggttcacctg gaacatattg atgtaattct
    41881 tttacggtat ttctcaataa tttttctatc atttttaaaa gccccctctg tatatgtatt
    41941 ttcatttata taaacgctat tgcgtctact tactttaata gcttaaatta accatctact
    42001 caactttctt ttaactaagc atttctttga tttaactctt ctcttatttg gtttaatttc
    42061 ttgtcactta ctggatatag tattgctata acaacagcta ttattaaagg tattgccata
    42121 gaataagaaa tcatatttat aaaacctgat ttaactgctt ctgtgactac catatcagct
    42181 tgatatccca ttgagttcat taaaactgtt cttatagtag ttgcaagtaa tactcctact
    42241 tgtattgcaa ctggatatag tgacatactt aagccaggta agtcttttcc tgatttccaa
    42301 tgcccataat cacaagaacc ggcaaagaaa ttaagattta ttattgatac tacatttgaa
    42361 aaacctccac caattgccaa caacatagtt actcctaaag tagttttccc ccaaggagtc
    42421 catgcaacca ataagtggca tagtgcacat atagaaaaag aaattattga agctagtttt
    42481 tgctctctac agatttttat aaacactggt gcgaaaaaca tgaatgctac acttaccaaa
    42541 ggcatgattg ataaaaatac tgctaatcca cgttcatctt ctattacata tttaaaataa
    42601 tatggagcca aaggtcctaa tcctatagat cttatacata taagaaattc taaacaaaat
    42661 gccatcataa gtggtacatt agtgaataaa gcttttgcca tttcacttgc tttaatcttt
    42721 ggcttctctt ctttttgaag aacttcttct tctgctccag attttattat ctctgttgaa
    42781 gaaaaccaaa atactaaaag gcatcctgct gcaaagaata agtaggtacc taaataaccc
    42841 attccttctc cacctaatac atctgcaaat aaaactaata ataacggata tgaaaaacct
    42901 actaagaatt taccaacttc tttaaatata ttcgtagtag ttgagcctat aactcggtct
    42961 ctaggtgtct tagcaactac agggtaaata gcataaatta tagtgaaaac tacttgctga
    43021 gcacaagatg ctattaataa taaagctgtc atcaaaggta aaagtaatgc tggacttatt
    43081 ttcattaatg gaactatgaa aaatattcca tatgttaatg ctaacgctgc tggtgcatat
    43141 ctaagccatg gataatactt tccttttttg aatggttgtt tgtcaaatac tgtacctgtt
    43201 ataggagcta cagcaatttt tatgaaagat tgtataattt gcataccacc tgccataatt
    43261 acaccaagtt ttgccacatc agttaaaaaa atatttgtaa cagttgatac aaagtaacct
    43321 ataaaattgt acgctaaatt tatagaactt gaatatgtgt atattgctct tttcttaggg
    43381 tctctcatat catctctaaa atttgatata agatttcctt ttggtttgct ttcttgacta
    43441 ctcacatcta cccctccctt ataaaattta cttacttttt attattcacg tataataata
    43501 tatattttga atcttaaata tctcattctt gattcaggag tatattttta tatgtgtata
    43561 attttaattt aattatattc tcaaaattca taattgtcaa acacttttta agttttattt
    43621 tttactacta aaattaacta aaaagctatt tagacttaat ttaggtttgt tttttattat
    43681 tttcttgttt tgaaatgtca ttgaaatgcg tattttaaat tattttatca tatttctagt
    43741 gattttaatt ttttataatt ttattcaaga ataatttatt caagatattt ctatactaca
    43801 tccagatatt aaaaatatct ttttcttatt taaattcctt ttatttttat acatgtacta
    43861 ttgatttaaa aataaaagcg tgttacaatt acaaaaaaga atctatgaga taaatatcaa
    43921 tatgcaaaaa tttcagaatt ttcaattttc tatttttacg taatttccat ctaatatcat
    43981 aaattattat agctatatta atctaaaacc aataaaacaa atatcgtact acttttatat
    44041 aaaatatatt ttaaatatat tttgtatatt ttattaaaat attttataaa ggagaaaaga
    44101 ctatgttact tttaattgtt acgtgtgcaa tatacatatg gattggtttt tatttttcaa
    44161 aaaaagttaa aacagcatcc gatttcctaa tcgcaggtag aaatctgcct ttgcctatac
    44221 tagctgcaac cattgctgct acatcttttg gaggagggtg cttagttggt ggagttcaat
    44281 ggggagctga aagaggactt tgggttggaa tgtattctac tataggtgca gctttagctt
    44341 gctttataaa tgcatttttc gcaggaaaat taagaagctt atcttgtgat ataacacctg
    44401 ctgattatat tgaaactaga tatggtcata gtgtattttt aagagcttat caaagtcttg
    44461 taacaccaat atctatacta gctattatgg gtagtcaatt gatttctttt ggttctgtat
    44521 ctactgcatt tggtattcct tatgatatag ctgtaattat aggagttata gttatcatta
    44581 tatatactta tacatcagga atgtggggag ttgcagtatc agcatttatt caacttgcta
    44641 tttgtatagt gtttttgcca atagtagctt atataagctt aaaattagtt ggcgacaacc
    44701 ctttacttat gttacaatca atggtaaaag aaccattctt tccaggaaat aaatcgatta
    44761 gtgattttat gtatacagtt ttaccattag ttttaggctc aatattttct tatgaaagtt
    44821 ttttaagata tcaatgtgct gataatgcta aaaatgcagt taaaagtagt gtaattgcag
    44881 gatttatact attatttctt gctttcccta taggaataat tggagctatt ggtggtagtt
    44941 tatttccaaa tatatcatct gttcaggttt tacctcaaat gatatcgact actttgccac
    45001 ctttaatatc tgtattattc ttagctgcta tattggctgc cataatgtct actgccgatt
    45061 cattaatgac ctcaatgagt gctattatat caagagatat ttataacaaa ttattacatc
    45121 ctaatataga atttaatgat ttaaaaaata ctctaaagta ttctcaaatt gcatcagctt
    45181 tgacagctat aataggtgca atcatagcac ttaaatttga tagtttatta gaattattgt
    45241 tttggccagc tccattatgt acaggtgtaa tatttgcacc atttgtaatt ggtctttgct
    45301 ggaaaggtgc tacaaaaaaa ggagctatct atgctgttat agtaagtgtt atattggcat
    45361 tattaaatat gattggtatt cttactgtat ttgacagaat tttggttcct ataataggtg
    45421 gttctattac aatatttata gttagcaaat tttcaaaaaa agattgaaaa gagaggtata
    45481 cacagtaaat atgtcaaaat cagaaaaggg caaagaaaca aaagctagta ttataagtgc
    45541 tgctagagaa ttattttttg agcaaggtta tcataagaca acaactaggc aaattgcaga
    45601 aagagctaat ataaatttag gtctaataag ctattacttc tcaagtaaaa gcgaaatagg
    45661 agctataata tatgaagata taagaaatga aatgcagtct ttaatctata actaccatga
    45721 agaaggtact atgatgatgt tctttcttgc tatgaatgct gacctttgtt tccttctaaa
    45781 aaacgaagca tatagaaatt tttatttgga tatagcttat gaagaagcta ttttaagcta
    45841 tcaaagaagt gtaaccgaaa tggtcatgaa aagatatgtt aatgataaag aatctataga
    45901 acatgatgaa ttaatacttt cttgtattgg tatacttgct atgaaacctg aaataattag
    45961 agtttatact tcacacaagt acgatatatc ttctcaagct gtaataaagc attttacaaa
    46021 acagtttctt attaatcttg gtcatagtac ggatttatgc gaagagattt tagaggaatt
    46081 gagtaaatac tatatagata tagttgacaa ttttactcca ataataactc gtgtaagaac
    46141 ttaataaaat tttattaaat tcttagagac tgaaatttta aaatcataat ttgattttag
    46201 gatttcagtc tttatcatat ttaaagatat tttatagtat acctgttaat accataagtg
    46261 tactatccaa ttatatctat aaatttcttt atccataaat tccaaagacg ccattcatgc
    46321 ccccctacac actcttcata ttcaacatct acacccagag aatctgcata ttttttaaac
    46381 atctgattat ttttatataa aaaatctcta tcaccacaaa attgataaat tttaggaata
    46441 tcttcatttc tttctttaag gttcttaagt atgtaaagta aatcattctc actatttcta
    46501 gcgtcttctg gtctaccaaa atcgtgaaat agaatgtctg cattggtttc tatttgttga
    46561 ttttctaact catgaatcat atcaggcgaa ccagaaaaac ttgcaactgc cgaaaactct
    46621 tttggttttg aaagcgctaa ttttaaagct ccatatcccc ccatagacat accagctaca
    46681 aagttatctt cccttttatc tgataaagga aatatagctc taacaaattt aggtaattcc
    46741 tcgcttacaa aagtaaaata ttggtgtcca tacgccatat cagtataaaa actagtttct
    46801 actcttggta ttactagggc tatattttta tttttagcat aatgctctac attagtatat
    46861 ctaatccatg agctttggtc atcttctaaa ccatgaaata agtataatgt tttaaattta
    46921 ccttcaaaat catatatctc tttaccacta aaaagttctt ttccatttgc tacaggcaaa
    46981 attacaatta tatcagtttc tcctccagta tatttagatt taaatttaca ttctataaat
    47041 gccacaaaaa acacctcttc tataataatt ttatatctaa aaagcaccaa gagtttaact
    47101 ttctcagtgc tttaaatatt gcttaattta tatattttta attatacata atatttttta
    47161 gctacatata atcggaagta tctgtcggta atcccttttt gtccaattta ctttcaacaa
    47221 tatccttaat tatagaataa attactgcaa acattggaac tcctataacc attccaataa
    47281 aacctaataa ttttcctgca accaataagg agaacaaaat ccaaaatgca gatataccaa
    47341 ttgaatctcc aagtatttta ggtcctataa tatttccatc tatctgttgt attattaata
    47401 taatcaacaa taaccagaaa gcttttatag gagatacaaa catcactatt agcgtagatg
    47461 gtatagctcc aaataaaggt ccaaagaaag ggataatatt tgtaatacca attataactg
    47521 atattaataa tgtatatggc attttaacta acgtaagtat tacaaatgtt agaataccta
    47581 taatgaatga atctaatatt ttgccaccta aaaatcttcc aaatgtatag ttacttctat
    47641 gagttaattc taaaattctg gcagcttgtt tttctgtaaa tactgcataa gttatttttt
    47701 tgcttaatcc atagaatttt tctttatcaa ttaataagta cactgaaaca attaatccta
    47761 gtacaatatt ccatatacta gaggctacat tctttaacat acttcctaat attgggatta
    47821 aatctgttac aaactttata atatatgtaa caaattcatt ccacttatca actgccaaat
    47881 taaagtattg ctcatcaaga tttaaatcag tcattaattt atcaataagc ttagttgcat
    47941 tgtttacata cataggtata tcatttgcta gtccaacaat actctcaatt acttgtggca
    48001 atacgaaatg cataaataag taaagtatca atgtagctgt tgcatatgtt aataaaagac
    48061 ccaagcctct ttttgaactt tgttttagtt tttttaattt ctcacttttt ataaaaattc
    48121 tgtcttcata aaactttaaa ataaaattaa gaagatatgc aatagcaaat cctattataa
    48181 aaggttgtaa tgttgtaaat atagcatcta aattattaga aaatacatca atttgtgatg
    48241 atactagata aaatataata ctactagcag ctattataaa tgtataaatt gctattgttg
    48301 tgtattttgt attccaatta attttcattt tttcctcctt atttctatat aattaaattt
    48361 tatcacaaaa tcaccacttt tataacaaat ataccaaatt aaaaataaaa tatattcaga
    48421 atacaaatac tcaattttat ttatatttat tattctacaa tataacctac attatctttt
    48481 taagaacctt aattaattta ttatcgtatg gagcatatct aatagggaca tccaaattta
    48541 atttttttaa cacactctta tagtgagtaa atgtatcgaa tccagctttt ccatgatatg
    48601 agcccatacc actttcacca ataccaccaa aaggtaagta attcgtagct atatgagtaa
    48661 ctgtatcatt gatacaacat ccgcctgctg gaatcatttc taaaagttta ttttctaggt
    48721 acttattttt tgtaaaaata taaagtgcca atggatttgg catttgtata attttttgta
    48781 ttacttcatc taaatcttta tatgttaaaa tgggaaatat aggcccaaat atttcttcct
    48841 tcattatatt atcatcccaa ttaacattgt ctactatagt tggctctatc tttaatgtct
    48901 ccttattata atttccacca tagactatct tatctttatc aataagagat aatatccgat
    48961 taaaatgtct ctgatttatg attttaggaa attgctcatt attacatgca ttatccccaa
    49021 aatacttatt agtataatat tttattttct ctattaactc attttttcta tcttcatgca
    49081 ctaaaacata atctggagcc acgcaggttt gtcctgcatt tattagtttt ccccaagtaa
    49141 gtcttttggc agtaaggtct atattagcat cttttaaaat gatacaagga ctttttcctc
    49201 caagttctaa agtaatagga gttaaatgct ctgatgctga tttcatgatt attttaccaa
    49261 cattagtact tcctgtaaaa aatatataat caaatctttc caatagtaac ttttcacttt
    49321 cttcttgact tccttgaaca actgcaacat attcttttga aaaagtatct tcaactatgt
    49381 ctttaactac actagatgtt gacacagaat attcagaagg ttttaatata acacaattcc
    49441 ctgctgctaa tgaccaaact aatggtataa gtgctaattg aaatggataa ttccaaggtg
    49501 acattatgag agtaacacca taaggttgtg gaacaacttt ggatatagat ggaaaattaa
    49561 ccatacttga tttaactctt tttattttgc tccattttct tatattcttt atagcaaagt
    49621 ttaattcact ataaaacatg cctacctctg tcataaaacc ttcaaaatcc gatttattta
    49681 aatctttctt tagtgctaaa agtatttttt cttcattttg tgatacaact aacttaagtt
    49741 tcttcaactg ttctatacga aacgatatat ctcttgtttt acctgtatta taatattttc
    49801 tttgcatttt aactaactct tttatatcca ttgttaaaaa tcctttcttt aagaattata
    49861 ctaatacatt tttagaaaaa gatttaaatt tatcctaaca ttatttttta tatcataata
    49921 aattcatctc aatataacat aaatcttcca ataaaaaaag ccccttatta agtataagta
    49981 agcttataaa atagtttatt ttaaaataaa cattttttaa aattataacc ttattgaaac
    50041 aattaaatta tccatcttta ataatattat tttataaata atgtctagta agtttctatg
    50101 aaatctaact ttagataata aataattaca aatatatacc tcttaaagtt ctcttatata
    50161 atcaagtatt ggagttaaat tttctttatt acaccaatca aaggcttctc cagacaataa
    50221 aggtgcttct gctggtttaa gaccttgacg ttctttttta gctatagatt ttttaaaaaa
    50281 actacaaaga gtataatcta taaaagacat ttttgtctca tcccatgccc cacgacaata
    50341 aaagcaagga attttgttta attcatcctt aaaatttact tctcttaatt tctttattac
    50401 ttcatcatca tatggagcag ctcctacgcc aaatactact aaattttttt tctgcaattc
    50461 tttataattt ttttttaaga aagaaattcc cgctatatta caagcataaa gacctcctcc
    50521 taaaataatc gtatcatact tcataacctt ttctatatta atattcttag tttcaaaaat
    50581 atcacaagac aataattctg aaagccattt agcatatttt tttgttgaac catacttact
    50641 cttataaatc actactacat ttttcatata aattatcccc ctctctgatg tcatacaaag
    50701 ttgatttgat acttgcttat tctccaacgt cattattttc tacataattt ttcacatttt
    50761 caattcctat acttaatttc ataattccaa aaaataactg ttccacttct ttctctgtaa
    50821 aatcttcaaa taaaagctgt aatacttttt cctgcatacc tcttactttt ttagaatact
    50881 cttgcactct atcttttgtc acaagacaga tagctcttga atctttttca ttctgctcaa
    50941 tttgtataaa acctttcttt tctaaagcaa tagctagttt ttttacattt tgtcttgagc
    51001 accccattaa ttctccaagt ttggttaatg taagagtttc atctacagag tccgcaatag
    51061 ctaaaagtaa ccattgtttc attgttaatt ctgggtctaa tttatcacat gtagtttgaa
    51121 gtttattttg cattacaaat atacttgcaa atatagcttg ttttctacta tcatcatcta
    51181 ccatatattt tttcattaaa ctttctgcat ccattattat acacttctct ttatcttaga
    51241 ttttaatttt ttgataattt taaatagtaa ctaattactt tatattatag taattagtta
    51301 ctattatgtc aataaattta tttttattat aaacggcaaa acggcatagc attatggcta
    51361 taccgtttca taaaatcata tttacaatca atttataata aattataaat taaatatatt
    51421 ttaggctata ataagttttt caaaatttac tctaaagcat actttttata cttattatat
    51481 tctatattgc tacattctac atctaaaata gctccatctt ctctgtattc tgtatttata
    51541 atgctcgtat tttcgttaaa ataagaaact acatgacctt tatcatatgg aattaagaac
    51601 ttacatctta cataatcttt aaatatgcta tcacaaatac attttatcat tttatctata
    51661 tttatatctt ttttaactga aacaaatata ccttctttat cgatactatc taaaattcta
    51721 tccaatactt ctacatctat taaatcaatt ttattataca catgtatcat aggtatacca
    51781 tctgcaccta tttgttttaa agtatcttct gtcactttaa tatgactttt atagcttgga
    51841 tttgaaatat caataacatg taacagtacg tctgcctcac atgcttcttc taatgttgac
    51901 ctaaatgctt ttactaagtc atgtggtaga ttacttacaa aaccaacagt atcagataat
    51961 aaaaattctt tgttatttgc aagaactata tttctgacag aagtttcaag tgtagcaaat
    52021 aacatgtttt tctcaaacac tttcttttct tcagagtttt taaatatatc tactagttta
    52081 ttcattattg aagatttacc agcatttgta tacccgacta gagctacatt tggaagattt
    52141 gattttcttc tcatacttct ttgagtttct ctttggaatt ttaaatcatc caactcccta
    52201 tttaaagatg ttatcttttc ttctattctt ctacggtcaa gttctaattt tttctcacca
    52261 gaacctctat ttttagtacc aactccacca ctttgacgtc ctaaattttc atttgaacct
    52321 ataagtcttg gtaacatata ttttaaactt gctacttcta cttgaagttt agcttctcta
    52381 gtttttgccc tttgtgcaaa tatatcaagt atcaatgccg ttctatctat aatttcacaa
    52441 ttaagctttt cttcaagatt ttttagttgt gatgtagaaa gttcattgta aaatataact
    52501 atctctatat tttctttctt tattaagttt aatatttcct caactttacc tgtcccaatg
    52561 taaaatgctc tattaacctg ctttgcattt tgagttatac tccctacaac atcaatatca
    52621 catgctgaac ataagttttc aagttcaatc attagttcat taaagtcctc agatttatta
    52681 tttatgttta atcctactgt tattcctttt ttcgccatat ccatattctc cttatctgct
    52741 attttttatt taatctatag cacgagatta tggctctata attaaattca tcaagaattt
    52801 tgctttttat cttatcctta aatgacactt cttatttact attataataa ccataatcgt
    52861 gcattatttt ctcaatctta tataaaaaat cactttctta cacctcctcc caaatatatt
    52921 atgcagtttt ttcaataaaa aaactcctta caaatctaag taatacaaaa caagctactt
    52981 atatcataag aaggattaaa attctaaata tatattaaaa atacattagt ttaattaact
    53041 tatatgtaat tatcatttta tatttaaccc tattcttaaa tttaagtagt tatttgcatg
    53101 attaataaaa gtaataaaaa catatacttc aatataatca tcaactatta aaattaaatt
    53161 ctatttataa taaaatttaa agaataaaat taaatataca ttttgataat cacctccaac
    53221 attttcctta ctacatatta taataaaata aacactttgt aaagacataa aattatgtgg
    53281 ctatctcaaa atatagagaa tcctcttttt cattgttaaa atgataaaaa tacactttat
    53341 ttcatacaca aaagagtgcc ttatgaatta aaaagttcat tttaaggcac cctcatttat
    53401 tttatcttat taattacatg tctatgctaa gatagttttt tgttacattg cttcatcttc
    53461 ttcaaattga gaattgtaaa gattggcata gaatccgcct ttctttagaa gttcctcgtg
    53521 atttccttgt tcaactatat ctccttcatt cattacaaga atcatatcag catcacgtat
    53581 agttgataat ctgtgggcta taacaaaact tgttctacct tccataagat tatccatagc
    53641 tttttgaatc aatacttctg ttctagtatc gacagaactt gtagcttcat ctaatattaa
    53701 aattttagga tcagctaaaa tagcacgagc tatagtcaat aattgttttt gaccttgaga
    53761 aacattactt gcttcttcat ttaatttcat cttatatcca tctggtaatg tcttaataaa
    53821 tctatgaaca tgtgcagatt tagcagcctc tataacttct tcatctgtag cattcaactt
    53881 accatatctt atattttcca taattgaacc actaaataac catgtatctt gaagaaccat
    53941 accaaacatt tctctaagct cactacgatt aaaatcttta atattatgac catcaatcag
    54001 tattgaaccg ctattaacat cataaaatct cattaacagt ttaactattg tagttttacc
    54061 tgctcctgta ggtccaacaa tagcaacttt ttgaccaggt ttaacattta ctgagaaatc
    54121 atttattata gtcttgtttg ggttatatcc aaagttgaca ttttcaaaat caattttacc
    54181 ttctaaacca tctattgaaa ctgcattttc aacaatttgg acttcttctt cctcttctaa
    54241 aaactcaaat actctttcag aagctgctgc tgttgattgc aataaattgg caacctgtgc
    54301 aatctgagtc attggctgtg taaagtttct tacatattgt ataaatgact gtatgtcacc
    54361 aacctcaatt gttttcttaa ttgcaagcca acctcctaag attgatacca ttacatatcc
    54421 caagtttcca ataaacatca taagtggttg cattatacct gataaaaatt gagatttcca
    54481 cgctgaatta tacaggctat catttagttt atcaaactct tcaattactt catcctcacg
    54541 attaaatgct tttactattg tttggccact gtaagtttcc tcaacttgac catttacatt
    54601 tcctaaatat tcttgttgat atctaaagta tttttgtgat ttctttatta ttcttgaaat
    54661 tacaaacatt gatattggta atattaaaac tgccactaat gtcataattc cacttataga
    54721 taacatcatt atcaaaacac ctatcatagt tgttactgat gttataagct gtgtcatact
    54781 ttgatttaaa ctttgattta aagtatctat atcattcgta attctcgata atatttcacc
    54841 atgcgtctta gtatcaaaat acttcattgg caatctatcc aattttgctg aaatctcttt
    54901 tcttaaatta taggatactt tttgagaaat tccactcatt ataaatcctt gtataaaaga
    54961 gaacagtgca cttatcaaat aaaggaaaag taaaaaggtt aagatttttc caattgcatt
    55021 aaaatctata cctacgtttc ctccagaaac tttactcact agcccttcaa atatctttgt
    55081 agttgccttt cctaaaattt ttggacctac tatagaaaac gacgcactac caatagcaaa
    55141 tacaattact agaattattg atattttaaa tttagataaa tatgcaatta gttttcgcat
    55201 agtcccttta aagtcatttg ctttttgtcc tgctcccata gattttccca taggacctct
    55261 tcccattcta ctcattagct agctcctcct ttgaaagttg tgataaagct atttgctcat
    55321 aaacttcgca attttttaga agctctttat gtgttcctat tccaactatt tcgccctcat
    55381 ttaaaacaat tatctgttct gcatttaaga tagtactaat tctttgtgca actattagaa
    55441 ctgtactttc agcagttttt gattttaaag ccttacgaag agctgcatca gtttttaagt
    55501 caagtgctga aaaactatca tcaaatatat aaacttctgg attttttgct attgctcttg
    55561 caatagaaag tctttgtttt tgacctccag atacatttgt tccaccttga gaaatttcag
    55621 ttttaaaagc ttccggttta gaatctataa attctgtagc ttgagctatt tcagctgctg
    55681 ttctcatttc ttcatctgtt gcgtcttttc taccatattt taagttggat tctatagtac
    55741 cagaaaacaa tactcccttt tgaggtacat atccaatttt ttcccttaaa tcatgttgag
    55801 aaacattttt tatattaact ccaccaagta atatttctcc ttcagtaaca tcaaagaaac
    55861 gaggaattaa atttataaga gttgacttac cactaccagt acttcctata aaggcagttg
    55921 tttctcctgg ctttgctgta aaagatatat ttgttaatat aggttcttct gcgtctggat
    55981 acatgaacga aacattttta aattcaacaa gacccttttt atcttcctta aactcttgtg
    56041 cctctgattt ttctgggtct tttatgctta tatctgacac taatacttca tctatacgtg
    56101 tagctgaaac ggatgctctt ggaagcataa tagataccat tgatatcatt aagaatgaca
    56161 ttattatttg cattgtatat tgtataaatg ccatcatatc tccaacttgc attgctccag
    56221 aatcaacact gtatgcacct ctccaaacta taagaactgt gataaggttc attataagca
    56281 tcatagcagg catcatacat gtcataactc tatttacaaa aagatttgtt ttagttaaat
    56341 ttttatttgc tttatcaaat ctttcttctt catgtttttc agtactaaaa gctctgataa
    56401 ctggaatacc agtaattatt tcacggctta ctaagtttaa cttatcaaca agcttttgaa
    56461 ccagtttaaa cttaggcata accactgaga ataacactac aattaatgat aagatagcaa
    56521 ctactgctac tgctataatc caggccattg aagtatttgt gtttaaaacc tttatcactc
    56581 cacctatagc cattataggt gcatagaata ctattctaag caacataacc atcaacattt
    56641 gtatctgttg aatatcattt gtactacgag taattaatga agctgtagaa aattcattca
    56701 tttcagtatt tgaaaatgac ataacttttc taaatactcc acttcttaaa tttctaccta
    56761 cacttgcggc tactcttgct gctaaaaatc ctactgttat tgtagctacc atactaatca
    56821 ttgctatacc aagcatttta gcaccagtaa ataatatata attattttga gttttttctg
    56881 tatcaatacc tattttctca tattctgacc ttacatatga aacagagctt tgtgttatca
    56941 tactctctgg catactttca aagcctttgc tcatctcttt agtcaatgta tctaactgtt
    57001 cctttggcat tgctgataac aatttaaata tatcaacatc tccactttga gtagcttgtg
    57061 gtggaagttt gctcattaac tgagctttca tagcttttac ctctttacta tcgccctcaa
    57121 gccctgatac tattaaaata gctttaccaa aaataacatt taaatcatct atagtgtctt
    57181 tgtctttagt atttaactca taaagagatt catcttttag acctggatac ttatctttat
    57241 acttaacata atccttttta ttcagcaaag tataattatc tagtacttta tccttactac
    57301 ttttatccat aaataaagta attttattta attcactctc tctaatcaca gatggaacag
    57361 agttttctac tccaccttgt tgtataccta catttactat attggatgtg tattctggta
    57421 aagaaaggtc acataccgct tgaattacca atagacagac tattattact attgataatg
    57481 ccgatttctt taaatattta attaatttaa acatacattt cttcccttct gtaatattaa
    57541 ttagtctaat aacattaaat tgaaaaacat tattttaaac tttaaaaata tttttcatct
    57601 tcatgcttat gacatcctgc ttttaaatta tctcgtactt gcattagaag tcttctaagt
    57661 actatttttt cttcttcact aaatcctaaa aaaaactctc tttccaattc cttgttaaat
    57721 tcttcaactt tattacatat ttctaaacct ttgtctgtca aaaaaatcct agatactctt
    57781 tggtcctttt catcttgttt tctttccaca agtccagctt tttccatcct ctttatcata
    57841 actgtagttg tagatgcttt tactttcctt ttatctgcta attctttttg actttgccca
    57901 tctttcatct ttaaagaaaa aagtatttgg ggttgaccat gatataagcc taatttatca
    57961 aaataaaaat gagttcttat aaaatatatt cgcattaatt gcaagaaaac atcatttaat
    58021 ttttcttcag aattgctatt catacttgcc ctccttattt tttatattac ttagtcggct
    58081 aagcattatt ttactatact gcatgcattt tttcaatata tttataaaaa attcatataa
    58141 atttcattta ttaaatattt ttatatcata tagttataat tttaataaat aatagtaaga
    58201 tttttaatta gtcagctact tatatttttt attgtaaagc ttaaaataaa ccttttcata
    58261 tattttcaaa taaaaaagtt gtcccaaaat aaattttgaa acaacatcat attcaacagt
    58321 atcttgatta tataacatta tgatactcta cttatcttca tatccattta caacaacgtc
    58381 taacttccca gtttcaggtg atataattag accatgtaca ataataccat ctggaattaa
    58441 aggatgccct tttattaaat caatactatc ttttacagat tcttctgcag aatcgaatcc
    58501 atgtaaccat tgttttgtat caattccagc actacgaagt gtaagtatta catcattaga
    58561 tattcctcta tctaaaattt taccaagtaa cttatccgta tcaacattac acataccaca
    58621 tccatgatgc cctacaacca taacctcatc tacatcaaat tcgtaaattg caactaaaac
    58681 acttctcata atacttccaa aaggatgcat tatagtagct cctgcatttt ttattagttt
    58741 cacatctcca tttttcaaat tcatagcctt aggcaaaagc tcagttaatc ttgtatccat
    58801 acaagataag actactattt ttttatttgg atgttttgat gtcacatatt gttcatattc
    58861 tttgttctct acaaatgatt tattatattc taatatctct tcaagttttc tcatatgtaa
    58921 tatcaccctc tttaaaaatt tttttattat tatatcacta ttaaagtaca atatatacac
    58981 atataattta atttatattt attaaat
//
LOCUS       DAYATN010000008        57053 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.008, whole genome shotgun sequence.
ACCESSION   DAYATN010000008 DAYATN010000000
VERSION     DAYATN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.008"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(245..1084)
                     /locus_tag="ACGI8A_000515"
     CDS             complement(245..1084)
                     /locus_tag="ACGI8A_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFH0178527.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1108..1866)
                     /locus_tag="ACGI8A_000516"
     CDS             complement(1108..1866)
                     /locus_tag="ACGI8A_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFH0178528.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1913..2386)
                     /locus_tag="ACGI8A_000517"
     CDS             complement(1913..2386)
                     /locus_tag="ACGI8A_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFH0178529.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2415..2831)
                     /locus_tag="ACGI8A_000518"
     CDS             complement(2415..2831)
                     /locus_tag="ACGI8A_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0178530.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIVINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2878..3930)
                     /locus_tag="ACGI8A_000519"
     CDS             complement(2878..3930)
                     /locus_tag="ACGI8A_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFH0178531.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4326..5006
                     /locus_tag="ACGI8A_000520"
     CDS             4326..5006
                     /locus_tag="ACGI8A_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFH0178532.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5139..5459)
                     /gene="sugE"
                     /locus_tag="ACGI8A_000521"
     CDS             complement(5139..5459)
                     /gene="sugE"
                     /locus_tag="ACGI8A_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFH0178533.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     regulatory      complement(5529..5635)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            5841..6668
                     /locus_tag="ACGI8A_000522"
     CDS             5841..6668
                     /locus_tag="ACGI8A_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFH0178534.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7490..8164
                     /locus_tag="ACGI8A_000523"
     CDS             7490..8164
                     /locus_tag="ACGI8A_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0178535.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8152..9312
                     /locus_tag="ACGI8A_000524"
     CDS             8152..9312
                     /locus_tag="ACGI8A_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0178536.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9549..10055
                     /locus_tag="ACGI8A_000525"
     CDS             9549..10055
                     /locus_tag="ACGI8A_000525"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFH0178537.1"
                     /translation="MTFSRIVFALLMLSSTFSSLPFWICYFWCGISDILDGLIARKLR
                     QQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYR
                     TFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELN
                     RDIKSIFM"
     gene            10341..11009
                     /locus_tag="ACGI8A_000526"
     CDS             10341..11009
                     /locus_tag="ACGI8A_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178538.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            11012..13480
                     /locus_tag="ACGI8A_000527"
     CDS             11012..13480
                     /locus_tag="ACGI8A_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0178539.1"
                     /translation="MKNYLSLSIKELKSQKFMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(13720..15144)
                     /locus_tag="ACGI8A_000528"
     CDS             complement(13720..15144)
                     /locus_tag="ACGI8A_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFH0178540.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15163..15855)
                     /locus_tag="ACGI8A_000529"
     CDS             complement(15163..15855)
                     /locus_tag="ACGI8A_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0178541.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15907..16686)
                     /locus_tag="ACGI8A_000530"
     CDS             complement(15907..16686)
                     /locus_tag="ACGI8A_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HFH0178542.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(16683..17453)
                     /locus_tag="ACGI8A_000531"
     CDS             complement(16683..17453)
                     /locus_tag="ACGI8A_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFH0178543.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17450..18154)
                     /locus_tag="ACGI8A_000532"
     CDS             complement(17450..18154)
                     /locus_tag="ACGI8A_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HFH0178544.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18409..18921)
                     /locus_tag="ACGI8A_000533"
     CDS             complement(18409..18921)
                     /locus_tag="ACGI8A_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178545.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSDCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            19247..19504
                     /locus_tag="ACGI8A_000534"
     CDS             19247..19504
                     /locus_tag="ACGI8A_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178546.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            19647..20273
                     /locus_tag="ACGI8A_000535"
     CDS             19647..20273
                     /locus_tag="ACGI8A_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFH0178547.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20520..21299)
                     /locus_tag="ACGI8A_000536"
     CDS             complement(20520..21299)
                     /locus_tag="ACGI8A_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFH0178548.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21367..21828)
                     /locus_tag="ACGI8A_000537"
     CDS             complement(21367..21828)
                     /locus_tag="ACGI8A_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFH0178549.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21804..22481)
                     /locus_tag="ACGI8A_000538"
     CDS             complement(21804..22481)
                     /locus_tag="ACGI8A_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0178550.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22714..23100
                     /locus_tag="ACGI8A_000539"
     CDS             22714..23100
                     /locus_tag="ACGI8A_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0178551.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            23103..24902
                     /locus_tag="ACGI8A_000540"
     CDS             23103..24902
                     /locus_tag="ACGI8A_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HFH0178552.1"
                     /translation="MYAPFFIRFLLSTLTLSILIAVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25257..27131)
                     /locus_tag="ACGI8A_000541"
     CDS             complement(25257..27131)
                     /locus_tag="ACGI8A_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFH0178553.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(27136..28800)
                     /locus_tag="ACGI8A_000542"
     CDS             complement(27136..28800)
                     /locus_tag="ACGI8A_000542"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFH0178554.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(28814..29635)
                     /locus_tag="ACGI8A_000543"
     CDS             complement(28814..29635)
                     /locus_tag="ACGI8A_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFH0178555.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(29734..30498)
                     /locus_tag="ACGI8A_000544"
     CDS             complement(29734..30498)
                     /locus_tag="ACGI8A_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0178556.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(30495..31244)
                     /locus_tag="ACGI8A_000545"
     CDS             complement(30495..31244)
                     /locus_tag="ACGI8A_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0178557.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(31748..32353)
                     /locus_tag="ACGI8A_000546"
     CDS             complement(31748..32353)
                     /locus_tag="ACGI8A_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0178558.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            32532..33170
                     /locus_tag="ACGI8A_000547"
     CDS             32532..33170
                     /locus_tag="ACGI8A_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0178559.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            33302..33964
                     /locus_tag="ACGI8A_000548"
     CDS             33302..33964
                     /locus_tag="ACGI8A_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0178560.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            33964..35145
                     /locus_tag="ACGI8A_000549"
     CDS             33964..35145
                     /locus_tag="ACGI8A_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0178561.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(35326..37986)
                     /locus_tag="ACGI8A_000550"
     CDS             complement(35326..37986)
                     /locus_tag="ACGI8A_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0178562.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(37983..38666)
                     /locus_tag="ACGI8A_000551"
     CDS             complement(37983..38666)
                     /locus_tag="ACGI8A_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178563.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(39010..39963)
                     /gene="blaCDD"
                     /locus_tag="ACGI8A_000552"
     CDS             complement(39010..39963)
                     /gene="blaCDD"
                     /locus_tag="ACGI8A_000552"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HFH0178564.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(40504..42618)
                     /locus_tag="ACGI8A_000553"
     CDS             complement(40504..42618)
                     /locus_tag="ACGI8A_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFH0178565.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            complement(42876..43736)
                     /locus_tag="ACGI8A_000554"
     CDS             complement(42876..43736)
                     /locus_tag="ACGI8A_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0178566.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            complement(44331..44693)
                     /locus_tag="ACGI8A_000555"
     CDS             complement(44331..44693)
                     /locus_tag="ACGI8A_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0178567.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(44927..45391)
                     /locus_tag="ACGI8A_000556"
     CDS             complement(44927..45391)
                     /locus_tag="ACGI8A_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0178568.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(45820..47241)
                     /gene="nhaC"
                     /locus_tag="ACGI8A_000557"
     CDS             complement(45820..47241)
                     /gene="nhaC"
                     /locus_tag="ACGI8A_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFH0178569.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            complement(47271..48335)
                     /gene="orr"
                     /locus_tag="ACGI8A_000558"
     CDS             complement(47271..48335)
                     /gene="orr"
                     /locus_tag="ACGI8A_000558"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HFH0178570.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(48320..49729)
                     /locus_tag="ACGI8A_000559"
     CDS             complement(48320..49729)
                     /locus_tag="ACGI8A_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HFH0178571.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(49729..51933)
                     /gene="oraE"
                     /locus_tag="ACGI8A_000560"
     CDS             complement(49729..51933)
                     /gene="oraE"
                     /locus_tag="ACGI8A_000560"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HFH0178572.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            complement(51927..52301)
                     /locus_tag="ACGI8A_000561"
     CDS             complement(51927..52301)
                     /locus_tag="ACGI8A_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HFH0178573.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            complement(52379..53809)
                     /gene="ortB"
                     /locus_tag="ACGI8A_000562"
     CDS             complement(52379..53809)
                     /gene="ortB"
                     /locus_tag="ACGI8A_000562"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HFH0178574.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSYFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            complement(53802..54110)
                     /gene="ortA"
                     /locus_tag="ACGI8A_000563"
     CDS             complement(53802..54110)
                     /gene="ortA"
                     /locus_tag="ACGI8A_000563"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HFH0178575.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            complement(54254..55321)
                     /gene="ord"
                     /locus_tag="ACGI8A_000564"
     CDS             complement(54254..55321)
                     /gene="ord"
                     /locus_tag="ACGI8A_000564"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HFH0178576.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(55577..56950)
                     /locus_tag="ACGI8A_000565"
     CDS             complement(55577..56950)
                     /locus_tag="ACGI8A_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0178577.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGREITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
BASE COUNT        19355 a         9708 c         6754 g        21236 t
ORIGIN      
        1 aaatttatat ttttttgaaa ttacttaata ttttatataa aaatgaaaaa gcacctcatt
       61 ctgattaaaa atctttctga gatacttttt aaaatattaa atactacata tagcttacat
      121 aagtttaaaa tttaatctat tataaaatta ccctaattgc caaacttttt aatataaatt
      181 ttccaacatc ctacaaatat aatcaataga tatagaatcc taacttacac caatatatat
      241 ttatttaaaa tagtcctaaa atacctgtaa tagctcccac tactatcagt ataaacatta
      301 tttttattgg agaaagacct ttttttaatg caaagagtac taataaagta agtaccaaag
      361 gaattaatcc tggaaatatc ttatcaagta aatccgtttg aagactaaat ttaaaatcac
      421 cactagtata acttattggt gtagataaat tgataaacct acctacaagc ccccccatga
      481 ctaaggtacc tagtatagaa gccccttcta taaccttatt tactattcca ccttgaagta
      541 tactttcaac agcttttttc ccatacttat atccatttgt ccacattcca aaagaaattg
      601 aagtcattat tatatattga gctattacaa ataatatagg tcctgcaatt gatgctcctc
      661 cctcagttaa accaatacaa acagctaaca ctataggagt tataactcct tgagttaaag
      721 tatctccaat tcccgctagt ggacccatca agcctgtttt gatagcattc ataccatctc
      781 cacttatttc atctgctcca ttagcttttt cttcttccat agctattgtt attccatgta
      841 taacacatcc acatataggt tctgtgttaa aaaatgctag atgagtctcc acttcttgtt
      901 ttaactcttc cttatctgga tataattttt taagtactgg caacattgaa tgtgaaaaag
      961 ctgttgattg caatctttca taattgtaat tagattgtgc aaagaaaaac catcttaacc
     1021 atgattttat tacatctttc ttgcttaacg ttttcttaga tacttcattt tttatatctg
     1081 acataatatt ttccccctca catgtattta agatgtagca ctatcttttc cgtacttaat
     1141 tgaagtaatt gtaagagcaa gtattactcc aataacagat attactatta tatctacttt
     1201 aaaataaaca gccattagta ttcctaatat gaaaaagggc atagtaaact tatttccaat
     1261 caattttaaa ttcatagcaa tcccaagtgc tggcaacata gccccaatta cattaaatac
     1321 atgtaacaca ttatcattca ttacttccaa cataccatct atcgcatttg gaccaaaata
     1381 aacaactaat aatgctggta taaaactcat tgcaaataac agtatctgtg gtggtactat
     1441 atttataaat gctactctat caacattccc tttttgaacc tctctatcag cccaatgtgc
     1501 aaaaaaagaa tttactgtca tctttccaat ccaaagtaca gttgcaacag ttcccaatgg
     1561 tacagctatt gccattgcag tacttacatc tagcttagat gctatagcta aagcagtacc
     1621 tacatatcct gcaaaagctg ggtcccccat ctgagctccc cctgctgata taaaacctag
     1681 atatatcata tttatacctg ctcctataat tgctccttga acagggtctc ctaatataat
     1741 tcccgttaca aatccagcca atagaggtct attccatgta aaaaatccaa cataagtcaa
     1801 ccacggactc caagaaagat aataaaaaat tgctattaat actgcttgaa ttagtgaaat
     1861 gtgcatattc ttcctcccta ttttctttta aagatttaca agatagtctt ctttacaata
     1921 atgttcctac atcaattttc tttgcatctg gtactatttg tataaagcat ttaacatctt
     1981 ttgatattag ctctctaaat atttcttttt cttcatctga cacagatata tttttataaa
     2041 aactttttct accaagccct gctcccatac ctccaacatt caattccttt aaatctaccc
     2101 cactatttac taaacctaag acagttttag ggtatttgac caatactatt aaattttcat
     2161 ctccattatg tgattttaag acttcagcac ctgattctac atcatgtact tcaaccttaa
     2221 cagttgatgg agctgccatt tgtaaaactt gaactataaa tgggtccttt actactaaat
     2281 catccacgat tactatcctt tttgctgatg taatcttaca ccaagcagta ataacttgcc
     2341 catgtattaa tctatcatca attcttgtta atgctatatt actcataatt attcctccag
     2401 atttttaatt tattttacaa ctttaaaaca gttttaacat ctttgattcc tgatatccca
     2461 acttcctcac ataaattctt caagtctttt aacttcatag tatctctgtt taaaaataac
     2521 tctaaaagca taggtaaatt tacacctgtc aaacattcta actcacatac cttagcaaat
     2581 ttatttatta caatagcaga tgtattaaat ggagttccgc cataaaaatc aagcaatact
     2641 aaaacccctt catccttata tttctcaatt ccatcttcta actttctact aaactcatct
     2701 atgctgtcac catggtttaa tcctaaagtt tctacatttt cctgtttgcc tactattaat
     2761 tcagcacttt ctaacattgc tttagccata tttccatgtg tggctataat tattcctgcc
     2821 atatgaatca tacctttctt aacctttttt atatggatta ttatttagta ctctaatcta
     2881 attatctata ttaatcatta tcttaccaaa gaaaatatcc cttttgtata ctttttcaaa
     2941 ataagctggt acttcttcta aatttattct atgtgttact aatttttcaa cattcatatc
     3001 acccttttgc atatgataca atccagcact ccattctttt cctgggaatg gaaaggagtt
     3061 tccaaaccaa gttcccttta cagttaattc acttcttact atcttttcaa attgctctct
     3121 agtcaaagct acatccccat atggtacacc tgcatacaat actgtacctc cctttttagc
     3181 tagcaacagt acttgaccac atgtcagtgg agtaccagcc gattcaataa caatatctgc
     3241 accatctcca tcagttaacc tttttatttc ttctacaata ttcttttctt ttgcatttat
     3301 acaaatatct gcccctaact cttttgccaa gtctaacttc tcatcaaata catccacagc
     3361 tattattttt gtagaaccaa atatttttgc ccattgtatt gaaaataatc ctattggacc
     3421 tgtaccaagt actacaacag tatctccaac tttagcttct gacctaaaaa gaccatgtcc
     3481 tgctatacat actggttcta gcgcagcggc agtctcataa cttatctcat ctggtatttt
     3541 tattaaattt ctctctttca cctttgtata ctctgcaaaa caacctccaa gttctttatt
     3601 tcctattatt gctacattat tacatcttga atacaagcct tttttacatt catcacattc
     3661 aaaacatggc atagctggac aaacagcaac tctatctcct attttaaaac tccttacttc
     3721 tttaccaacc tgtgccactt ctccagaaaa ttcatgccct aaaatttctc caaccatatg
     3781 tggacctgtc tttgaatatt ttgatatatc agaaccacat attccagcta cttttacttt
     3841 aattattaca tcatcttttt ctaatatttt tggtacatcc acatcctcca ctcttgtgtc
     3901 tcttatacca tagaatctaa ctgacttcac tattaaccac ccctactttt tatttgagaa
     3961 tatatcccta ttgtttatat gttaaatatc taaagcaact ttcatgccaa tatatgtaag
     4021 cattgtattc atgcatttta taaaattatc attaaatgtg tgtcaaagtg tgtcaaaagt
     4081 ttcttatatt aaaacttatg tgtgtcaata taaacgacac taacatttta tattagttct
     4141 cttattataa gttttataaa atgtactttt tttgttattt ttataaaatt gttcggaaat
     4201 ttcccttcta tttgtgatat aatattaaat ataaggtatc aatataaatt ttcatgcgaa
     4261 tatcagaact tttttgacac taatgcctta ttcaatataa taatttaact ttttggaggt
     4321 tgtttatgaa aaaagtgtat caaggaaaga ctaaagatgt ttttgaatta gataatggaa
     4381 attacttact taaatttaaa gacgatgtaa ctggtaaaga tggagtattt gacccaggcg
     4441 aaaatagtgt aggtctaagt atagatggaa ttggtagagc taatttagag acatctgtta
     4501 agttctttga aattctaaac aatgctggaa ttaaaactca ttatgtaagt gctaatttag
     4561 aagaagcaac tatggaagtt ctaccagcaa aagtttttgg taatggatta gaagttattt
     4621 gtcgttatag agctgtaggt agtttcttac gtcgttatgg ttcatgtgtt gaagaaggtg
     4681 ctaaactaga ttgttacgta gaagcaactt taaaagatga tgaccgttgt gaccctctta
     4741 taacatctga aggtttagaa gctttaaaca ttatgactca agctcaattt gattcaatga
     4801 aaagcatgac tcaaaaaatt tctaacatag ttcgtgatac tatagctgaa aaaggtcttg
     4861 agctttatga tattaaattt gaatttggat tctacaatga tgaagtaatc ttaatagatg
     4921 aaatagcatc tggaaatatg cgtgtttata aagatggtgt aatagttgac cctatggatt
     4981 taactgctct tttattagat aaatagtata tacatataat ttacaaatat aataaactta
     5041 taaataagat aagctatgaa gtaaagactt tttaaatgtt gattattttt aaacattttc
     5101 tcgcctttaa tttcatagct tatttaaatt aatatactct aattagtaga taatagcttt
     5161 aaccctacaa ttcccaccac aataaaacct atacatgcaa ttcttgatat actaataggc
     5221 tcatttaaaa tagccatgcc tacaatagct gtaccgacgg taccaattcc agtccatatt
     5281 gcatatgaag ttccaagtgg aagatgtttt agtgctaatg acaaaaaata aaaacttaca
     5341 atcattccaa caacagtaag tatacttgga gttaatttag aaaatccatg tgaatttttt
     5401 agttgaaaag cccaaaacac ttcaaacaaa cctgcaacaa acaaaaataa ccatttcata
     5461 aattcatccc tcttttcaaa tatgtctctt atttttccaa taaaataacc tgagaaatat
     5521 tataaaatat ctcccaggtt tttgtccttc cgtgttcaca acaaaaacta tgttgtgggt
     5581 tttatctcgg accagaccaa tcattaataa ttgcggaacc ctataaaact ctattaatta
     5641 ttctatttta tctataataa tactatcaac atatataaaa aaagtcaata tatgggttcc
     5701 atatataata aaaataactt ttatatacct tttaatattc ttattcactt ctctccatac
     5761 ccatttgggt caatctaaga atatctaatt tttatttaat atgttataat aacataaaaa
     5821 tattgaaagg agtaatttct atgaatttct ataaaattgc agtatgtcag atgattacta
     5881 cagaaaataa aattgaaaat atcaatcatg ctgtagatat ggtaacagaa gctgccatca
     5941 atggagctaa aattgtagtt ttgccagaaa tgtttaactg cccatatgaa aataaatatt
     6001 ttcctaaatt tgctgaagaa tatccaggtg aaacaacaac gatattaagt aaattagctg
     6061 agaaacatgg aatttatcta gtttctggta gtattcctga gttagaagat ggaaaaatct
     6121 ataatacttg ctatgtattt gataaaaatg gagctctaat tggaaaacat cgtaaaatgc
     6181 acttatttga cattgaagtt acaggaaaag ttagttttaa agaatcagat actcttacag
     6241 ctggaaatga tgtcactgta atagataccg aatatggaaa agttggtata gctatttgct
     6301 atgatattcg tttccctgag ttatcacgtt taatggctct taaaggtgct gaaatcgtta
     6361 tcttacctgc tgcatttaat atgactactg gtcccgccca ttgggagtta tctataagaa
     6421 tgcgtgcttt agataatcag atattctatg ttggtgctgc acctgctaga aatatgaatg
     6481 cttcttatat agcttttgga aattcaagaa tttcagaccc ttggggaaga ataattgctc
     6541 aagctgatga aaaagaatgt attatttatg ctgacattga tagagattta attcctgaca
     6601 taagacaaca gttaccactt cttaagcaca gaagaactga tttatatgaa cttaatactt
     6661 tgaaataaaa cagaacataa ccccataaat cataaattaa gtgttaaaaa aatttatggg
     6721 gttctcatta atttgattta aaattaattt cataaaaata atgctaatat taatttaatt
     6781 aaataatact aataattact aatgattatg ctaattatca gcatttacta atttaatcac
     6841 tattagttat ttcttttatt aaatatcttt acttttttac ttattatgtc ttctagcttt
     6901 acatttggat tatggtattt ctttctattt ttagtcacat ctttctattg aattacttgc
     6961 ttaaaacacc aatgcacaca tgccagataa tgttcacaat tatttagaaa tcttgctcta
     7021 ccttcttcaa tgttgatatt atctctccta gttgatatta tctttcctaa aatttctttt
     7081 aattaaaaaa caacattagt tgatatatca agtatatttt aagcaactga aaaaaacaat
     7141 aataattgat atatcaacta attaatgtag attaattcat ttttataatt tatatacttt
     7201 ttaattaatc taccttattt aattaaaatc cttcctttct atttatccat tttcaactta
     7261 aattataaga tttcatcagc tatatttttg cataccaata attattaaat atcaaatata
     7321 tattctatta aaatttctaa taatttcatc cttgatttat ttaatatata aaaatatact
     7381 aactttaatg attaagtttt gaaatataac taagaatttg ataatttatt gagattttta
     7441 tactaaaata tacatactat acaaacttaa tttagaaggg agcattctta tgaacaataa
     7501 aatactcgtc gtagatgatg aggaaggtat aataacactc ttaaaagatt actttgaaat
     7561 gaatggttat gaggtttaca ctgctcaaag tggaaaagac gcattaaaaa aaatatctaa
     7621 aaatccagat atcattctac ttgatataaa catgcctgaa atagatggaa ttgaagtttg
     7681 taatagaatt agagattatg tatcatgccc tattctattt cttacagcta gaattgaaaa
     7741 tactgacaag ataaatggtt ttcgtgctgg tgctgatgat tatattgtaa aaccttttga
     7801 tttagacgaa cttggagcta gagtatctgc ccatttaaga cgtgaacaaa ggaagtcaaa
     7861 tcaaactaaa attaaatttt ataatgattt aacaatagat tacgaaaaaa ggttaatttg
     7921 tatatataat aatcagattt cactttcaaa aaaggaattt gatatagtag agcttctctc
     7981 tatgaatcct ggacaagtat ttgataaaga aaaaatatat gatagagtct ggggatacaa
     8041 tagcaatgga aatagtgatg ttataatgga acacattaga aaaatacgaa tcaaactatc
     8101 aaaacatact cttgaaaatt atatagaaac agtctgggga gttggttata aatggataaa
     8161 ataaataata tgtcattaaa aaaagctttc tttgttctta ctttatcttc actaattata
     8221 gcatcagtgc ttactacttt cgcatacatt ttacttaata atctatataa ttctatacaa
     8281 gataagtatt taaaaacacc tataaataca ggtattataa ccatagttca aaacaatgtg
     8341 aaccaattta gtgattatga taaaacatta cttaatataa tcaacatact tcagcttgta
     8401 cttccactta tcttttttat aggactttta ttattagctg atataatttt ttacaaggta
     8461 aaattaaaaa cacccataga aatactcaat aaaggtgcaa tggaaatatc caacaacaat
     8521 ttagactttt gcttagaata taataataat gatgaattag gtaatctatg taatgctttt
     8581 gaaaaaatga ggtctgaact aaataaaaac aacataaaga tgtggactat gattaatgat
     8641 agaaaacaac ttaatgctgc tttttctcat gatttaagaa atcccctaac agttctaaaa
     8701 ggttactcaa actatttaac aaaatacata ccaacaggta agcttagtga tgagaagatt
     8761 ttatcaacca cacaacttat gtctgagcat attaacagaa ttgaatacta tgtagaaaat
     8821 atgagtaatg ctcaacgatt agaagattta gttgtttcaa aatctatgtc gaatataaac
     8881 aagtttatag aaaacttaga tgaaaatata tctatcattg ctaaacaaga aggaaaatct
     8941 tttacactta aaagtcaaat aaataatata aatctatttt ttgatgaaaa tatcattctt
     9001 agagtcgtcg aaaatataat atcgaatgct tttagatatg ctagaaataa tgtatccata
     9061 cttatatatc ttgaacaaga actccttacc tttgtaattg aagatgatgg tataggattt
     9121 agtaaggaat ctctaaaatt agctttaaga cctttctata gagataagac tttaaatgac
     9181 agcaatgcac actttggtat gggtctttat atctcaaaaa tattatgcga aaaacatggt
     9241 ggctctattt ctattgaaaa taattctact aacagtgcta aaattactgc taaattctca
     9301 acaagaaatt aaaatgattt tgtaagcttt cactgttctt atataaaatt tgttagttta
     9361 gaatttataa aatgtatttc aatttacaac caatatatat actattttgt agaagttgta
     9421 agagttgctt caattataat atttggcaaa aggcatttga aataataata gaaatatata
     9481 tttaaatatg tagaatagta tgaaattaga attttggagg tgattatatt aaaagttttg
     9541 ctaataatat tacattctct cgtattgtgt ttgcattgct aatgcttagt tctacattct
     9601 cttcactgcc tttctggata tgttattttt ggtgtggaat tagtgatata cttgatggtc
     9661 ttattgcccg aaagttgaga caacaaagtg gaataggtgc aaagttagat agtattgctg
     9721 atttcatttt tgcggttaca attttggtta ctgtcggaaa aaatattgct cttcccatgt
     9781 atctatggtg gtgtattgtt gcaatagcat tgttacggtt gattagttac agtattggtt
     9841 tttttaagta tcgcacattt tcatcgcttc atactttgat gaataaaatc actggaattt
     9901 ctatctttgc ttttccatta ctctatattt gctttggtct tgatgttgca ggaattatga
     9961 tatgtatcgt ggcttttctt tcttcttttg aagaaatagc aattatgata aaatcaaaag
    10021 aattaaatcg tgatataaaa agtatattta tgtgacaact tagaatttgt gacaaaaata
    10081 atttatataa taatgaggaa gtaaaacata aaaaattatc cacaagttta tgtgtaaaat
    10141 agttttaaag caatagagtt gtattgtaaa gcctttagtg cataaattac attttaaatt
    10201 aaaaatgata ctgaaactgc ttatcagcac tgcgaattat ccgcaaatag tgaagacttt
    10261 ttagatttaa gctaaaagtt gggaaaaaat tgagttttaa aacttacact cttcttatat
    10321 agaaaaggag agtgtttttt atgattataa aagcaaaaca attatcaaaa atatatggtt
    10381 ctaataacaa taaagttata gcacttaata atgtaaattt agaaataaac tcaggtgaat
    10441 ttgtttctgt aattggacct tctggaagtg gaaaaagcac acttttgcat attttaagtg
    10501 gattagataa tcctacctct ggacaagtgt tacttgatga taaagatata tataaacaca
    10561 ctgaaaaaga actatctgca cttagaagaa aatcctttgg attcgtcttt caacaattta
    10621 atcttttacc tgttcttaca gcatctgaaa atatttcaat gcctgtactt ctagataaaa
    10681 aacaacctga taaagactat ttaaatgaaa tttcatcatt acttggaatt gcagatagac
    10741 tcaatcatct tccccatgaa ctttcaggcg gtcagcaaca aagagttgca attgctagag
    10801 cacttattgc aaaaccagat ataatatttg ctgatgagcc tacaggaaac ttggacagta
    10861 aaagtggcag tgaagttatg aatcttctta taaaaacttc aaaacagttt ggaaagactt
    10921 tagtggtaat tactcatgat gatagaattg caaaacttgc tgatagaaaa atttcaatta
    10981 ttgatggtgt tcttatggag gtgaaataga aatgaagaat tacctttcac tttcaataaa
    11041 agaattaaaa agccagaaat ttatgactac attcatcatt atagcaatag tactatcaac
    11101 tattatgaca actgtggttg gacagtcaat tggtatccta caaaatttaa gaattgaaca
    11161 agcccgttct tttaatggtg atagacatgt atcctttcac caactcacta aaaatcaagt
    11221 tgatgacata aagaaagatg atagagttta tcaagctggt acttcaataa ctattggcag
    11281 tagtaaaata aaggattctg gtatctctgt acttgtaaaa gagtatgata aaacaggact
    11341 ttctaactac ccaaaattaa tgaaattaaa atctggacat ttgcctaaag ataaaaacga
    11401 aatagcctta gatgaaaaca cactaaagct tatgggtata aaaccaagac taggagtgac
    11461 tatacctatg aatttagata tttcactttt aaatgatacc ataccaccat ataattacac
    11521 agctaacttt aaattgagtg gcattttaga ggatgattat acaggatatg taagtggtat
    11581 agtaaatggt attgtaggta aaggtacatc agaaaatcta ttacctaaga gatatatttt
    11641 atcctctttg gattttaaaa taaaacagca agaaaagttt caagaaatag taaatcaatt
    11701 agctaaaaaa ataaatcttt ctcacaattc aattcaatat aattggattt acttaaatgc
    11761 tcttaagata cagtttgaaa aagatgaaaa tagttctaat agtgatggta tatctatgat
    11821 tatattggta tctttatttg ttgccttatt ggttttactg gcttctggac ttgtaattta
    11881 taatatttta aaaatctctg ttacaaagaa aattaaagag tatggatgtt taagagcaat
    11941 aggagctgaa ccaaaccaga tatataagat agttatttta caaatcttaa ttttatgtac
    12001 tgttgccata cctattggag ctgtaattgg aattatatct tctaaaggca taactggaat
    12061 ggtcactaat atcttaaatc cagatatact tctagcaaat gataataaag aaattactga
    12121 gcttatacat aaaaatacaa cagcatatat attcccatta gtattaagca ctaatgtatc
    12181 tttgattttt tcatttattt cagcattacc atcagcaata tatgcttcac atgtctctcc
    12241 caaaattgct atggttggta gtactaccaa aatcaaaaga aaaattaaaa gagaaaaaac
    12301 aataaaaaac tttgaaagac atctagcatg gctaaactta aaaagaaata aaggaagaac
    12361 tataataact atactatcat tatttatgag tataacagta ttcgttgcac tgagtgagtt
    12421 ctctaatgta ttagatgtat ctaggtcagt aagtaattta aaagaaggag atttttcctt
    12481 aacaaatgaa atatctggtt ttgataaatc aacactagat aaaatagcta aaatgaaaaa
    12541 cgtcaatcgc acttccttta taaagtactc tgaatacaaa cagggtgaaa tagatacaga
    12601 tataaatttt gaaaatagtg gtgagatgtt aaaaatcatt ggaattgatg agcaaacttt
    12661 aaaagactta atgccttcta ttacagattc tatactagaa gactttaaaa atggttctat
    12721 ctgttttata aaaaatccga ttgctatttc tacaccaggt gtaaaaacta gatatacaaa
    12781 tttaaaacct aaagatagta tcacaatcaa caaaaaacaa ctagatatat atagtacagt
    12841 agataaaatg ttctttttac aaggcaatgg ctggataaat ggtgttgatg ttattgtata
    12901 tgattctgtt tataacactt tgacaaataa aaacaagttc aaccagataa atatttatgc
    12961 taaagataaa tcaaaattag aacaaataag attgtctatt gaacagattt gtgaaaataa
    13021 tcctggctct cattggatat catatattga atcagataaa caacttaaag agagttttaa
    13081 acaaatagaa tttctagcat gggcagttat attatttgtg ggattgattg gtactttaaa
    13141 catcattaac acaactcata caaatataaa tactaggact aatgagattg gagttaaaag
    13201 agctattggt atgagtaata ggagcttata taaaatgttt ttatgggaag gtgtttatta
    13261 tggaattttt gctgctatat ttggttcaat tgctgggtat gccagtgcta ttatcataaa
    13321 catggcaact atagaaaagc ttgatttcac taatatacca attacttcaa ttttacaagc
    13381 tactattatt tctgtacttg catgtattat agctactctt attccgttaa gaaaagttaa
    13441 aaagatgaat ataattgact gtatagatat caatttataa ctattttaca gataataata
    13501 taaattaaat tatcaatgac tgtctctatt ttctttcaaa tctaactaat aaatcttttt
    13561 aactataata taagctttaa aaaaggcctg tctcaaaata gagataagcc ttttttcatt
    13621 gtcgaaataa tgaaaatata tttaacttca caaaaaaaga gtgcctcacg aattaaaaat
    13681 ttcgctttga gacactctcc tttaatttaa aatatatagt taaatgctat gccaaaattt
    13741 cacacatgct ccacctgatt ttgaattttc tattttaaca gaacctccaa gctgttccac
    13801 taattgtttg acaatgtaaa gcccaagacc agaatgtcca ccttttgtct gcctagcttc
    13861 atctcctcga taaaatttat caagagcctt ttttaaatct ttagagctaa accctctacc
    13921 agaatcacaa atctcataag aaatacaatt tttttcatcc tttacagtaa tactaatatt
    13981 accacctgat ggagtgtatt gcagactgtt ggatatgata ttatcaaaaa ttcgtgtcaa
    14041 cctatcaacg tcgatttgaa ttggggattg tatattcccc tgcattttca atataagttc
    14101 aacttccttt tgatgagcct gtaattcata gtcttgcacc ttttgcctca aaaattcagg
    14161 taaatttata ggaacaagat ttaattgtat atttgacttt tctaagtcag acgtatattg
    14221 catcttttgg acaagagcag cacttttttc tgtattttct cttatcactg taagatacct
    14281 atgaagtttt tcattgtcat ctgtattatt accaataagt gcatctgtat atcccaaaat
    14341 aatagaaaga ggagatttta aatcatgtgc tagagcttcc accatttcaa cacgttcctg
    14401 ctccatcttc cactgtgcag ataaagagcc ttttaattcg tctttcattt cagaaaaggc
    14461 actacaaagt ttaccgagtt cattatctga ataatagtcg atttcaaaat caaggtcctt
    14521 ttcttttatc ttcttggacg catcaatcaa tagatgtaat ggttgattga tattctttac
    14581 aaagcgctta gaaagccatc tagtaaaacc cacgatatat agaaatggag aaaacaaagc
    14641 cattataata actgcaaata caaatctccc tctaatattt gcaaaggtga ttttcacttg
    14701 gtagaataga ataacagcac cctcaatctt tccattatcc cctttgattg gaacagtatg
    14761 gatataacca cctttacgaa cagttttatt gataaaatta ttaaataatt cttcttttgt
    14821 tttaaatagt tttttggggt tagtaccata taaaatattg ccattattat ccacaacttg
    14881 atatagcata tcatccccac gaatcgttct ttttaaacct tcttcattcg attgtgataa
    14941 caaagctatg tttttttcat tgatgtattt ttcaattcta ggaacttgtc gctcataata
    15001 gtttggtgga tagatatcct tatttaagga atgaatatac aaatagagag caaatacata
    15061 agtaatcact gtggcaacta tactggctat tataataaaa ataaaagtat gtctaaattg
    15121 ggtaataagg gattgatttt tcatttaata acctctctct ctttacattt tattccactt
    15181 atacccaata ccccaaactg tggaaatata ttctttttct ggtgtcacag cagaaaactt
    15241 agctcgtatt ttttttactc gttcaacaac agttgaggaa tcaccttcag aatcacaccc
    15301 ccatactttt tcatagattt gttctcttga gaaaacttgt cctgcatgga gtgctagtaa
    15361 ttcaacaata tcatactcta tttttgttaa atcaatattc ttaccatcaa ttttaattgc
    15421 acgttccttc atatataagc ataacttccc aaaatacaaa gtagatctct tgttctcttc
    15481 attgatatac tgtgaacgct tttccctacg cagattagct tcaatccttg ccataagttc
    15541 acgcagtcca aatggctttg ttacataatc atcccctcca aaggtcaacc ctttgatttt
    15601 gtcggtttct gattgctttg cacttaaaaa tataattggg cataaaacat catctctaat
    15661 tgcctgacat acctcaaatc cattcatttt aggcatcata atatccagta taataaggtc
    15721 aggctcctgt tttgataact ctatacctgc ctgaccatca taagctatca acacttcatg
    15781 tccttttgcc ttcaattcat cctcaagtag cattactagg tccatttcat catcaataat
    15841 caatatttta ctcaatataa caccaccttt ctcttatggg aaggtcagta ttaaaacaat
    15901 attcgtctac tcataacttt tccttccctc ccacatttta aaccaaataa cgcctcctat
    15961 caagaatata atcaaaccta ttgctactac tataagctct atactagctg tttgcatcac
    16021 tttagagtaa aatgcctcag ttgaaatagt tccagcttct ttaataaaat aaattcctgg
    16081 aagcatacct agttttactg gccatgtcca tggaacaaat gcccatactt tttcaccaac
    16141 gctagttaat ccaagtattg ctgccatcaa aattccacca atactaatac caatagatgc
    16201 acccataccc catgcaaaac tcacccaaag atgaatagct aatagtggaa gtgtcccaat
    16261 caccacaagt cctgctgctg acaggaataa ccatattgtt gctccagatg gcactgcaat
    16321 actcattcct atgcaaagta tcaacgtagc aataaatgtg catatagtta ggcaaaacag
    16381 aagaaaaaga aattttccca gatataatct gacacgagaa atacctacac ataaaaaacc
    16441 attaaaattt ccagcaagct cttcctcctg cactataaat cctgctaaaa tacctactcc
    16501 aataggaatg atgaatacag tccatactgt aaaaaatcct tcaaaagcaa attcttgtgt
    16561 acttccagat ttagttgtga gataaaaaac cacacataat gaaaaaataa taggcataca
    16621 aagagtaagc cactggacag ctgtcctctt tgctcgaagc cattctgatg ataatagttt
    16681 tatcatttgt catccctcct attgaaccaa gctgcagtaa gtgctgttaa tactataaac
    16741 acaaccaaag aaacaacaat cccaatagga atcactgaag tattcaaaag tgggtctcca
    16801 gcttctaata tagtaccgtt aggatgtatg ttgatgattg ggcataccat tctaactgcc
    16861 caactccaag gtactgcaat ccaaaatggt tttgtcgcag caagaacacc aaatatcata
    16921 cctaaagctc caactcccat acttaggaac atacctttcc atgtagctag ccatagctga
    16981 agtggaagta tagcaagaga aacaacccag catacaatac ttgcccctat aatctgtcca
    17041 aaaggaactg gaccagcttt caatattaaa ccagttataa ttgttacgac tattaaaact
    17101 agcgttgaaa taaaactgta gactgccata gctataactt tattaatcca aagtgtcata
    17161 ggagacacat tgtgtgtacg taaagcacga taatttccag cttttttttc ctggaaagct
    17221 accaaagtag cgaacaatgc aaatccaata ggcagaaata aaaatgacca ccagttaaaa
    17281 attaatgcta aaagattttg ccacgcccaa gaagttgttt gagataatgg attttgcatg
    17341 agtgttgatt gcattataaa tgcatatgct gcaaaagaca ctgggaaaaa aacaataagt
    17401 ttacccataa atgttctttt atactttaaa agttctgatt gtaaagcatt catctgtttt
    17461 gtcctccttt aagaataaca tctgtaaaaa attcttctag attttcatta gggtcaggca
    17521 ttccttgaaa taatagtcgt cctccattta taattcctat attatctaca acttgagcaa
    17581 cctctgatag aatatggcta gaaagaatta cagttatacc ttttttggga aaagaagcaa
    17641 tcaattcacg caattcttga atgccaaagg ggtcaagacc attggttggt tcatctagta
    17701 ttaacaactc tgggtcactc aaaagtgcaa tagctattcc taacctttgt ttcataccca
    17761 tagaaaattg tgaagctctc tttttacctg tatttttcaa atttactatt tccaacactt
    17821 cataaatttt atctttaggt attcccaata tttttgtatg tactaacaaa ttttcctctg
    17881 ctgtaaggtt tccatacaat gcaggggatt ctattaaaga acctattttt gcaagagatt
    17941 ctcgtttcca aggctttcta tcaaaaataa tacgaccatt tgttgggcga agaagaccaa
    18001 caagcatttt aagtgttgtg gactttcctg ctccatttgg tcctaaaagt ccatatatac
    18061 tatttcgttc aatttgtaga gaaacattgt taactgccat ttgttctcca tatctttttg
    18121 ataagttttt tgtttcaaga attaaatctt tcatattaat ttctcccttc ttgtaatacc
    18181 ttaaatctgt tactaagaga atactaacat gaaaaaataa caattctata acacctacta
    18241 attttaataa aatttaaaat aaaaaataga gttaccaatt ttattttggc aactctattt
    18301 aagtttatgt tttacagtta tattagattt aaacttagtt tcattatcac actaacttca
    18361 aatttactct ataatcttta aatcaaacac taatttatta atcaaacact aatctattaa
    18421 tttatatttc tttagacatc taatatagtt tgctactaaa taataattat ttatatcata
    18481 tttatctaat cctttaaaag aagttatttc atcattaagc tcatcactac cttctacaaa
    18541 aacacaacct gtttcaaatt ccatacctac aataatctta cagtcactat aaatttcctc
    18601 ttgagtatcg tacaattttt ttgcatttcc ttcatttgga attgtaaata caataggttt
    18661 taattcttta tcactggaaa tcttttgtga aaaaggcttg tttaacttaa tattattgat
    18721 aactacatgt ttaaattcct ctgaagtttc ttctactatt acatattttc gctttaaaaa
    18781 ctctattatt tctaaaacag acttagaatt tacttttaaa atacttttat atttttcata
    18841 taaagatttc cactgttcaa ttttttcctg tggtatttct tggatatgtg tacttatttt
    18901 ttcttgagat atttttttca attaaactct cctttcaata cttaaatatc cttatgaatc
    18961 tcacatgact aaagttacaa ggtttctact taatagaatt ttgcatactt aaatatgtct
    19021 tattaattct ctacaggata tctccgtagt tcctacgatt cttacataag ttacttattt
    19081 taaattgtta ttagttatat tctaaaaatt atttatttta tcactaatat aatactctta
    19141 aaaactttac aaatcaattt aaatttgcta ccttttattt ataacaactt atagtacatt
    19201 tctcctatgc ttttcaatat attactttta aaaggaggtt ttcattatga ttttaaatag
    19261 agctgagctt ttccgaggat attctttaaa tagatttaac gagatatgca atatattatc
    19321 agtaaataaa attaaatata agtataaagt gaaaaataat gcttactcta aaaaaccttt
    19381 ttttaatgaa ataacacttg gcaatttagg ccaaaaggaa gatttctctt atgagtactt
    19441 aatttatgtt cataaagacg attatgaaca ggcagagtct ctaataaatt ctaagttgcg
    19501 atagattatc tataaaacat gacattctgt tgtcatgttt tatatgttat tatactttca
    19561 aatatgctac tatattttca aaattgtgtg ctttaaaact agcttttttt agtaatttaa
    19621 agcttaaaac taaggagact cgttatatga aaataaatat aaaatcaaag ttaagtgaat
    19681 ttattaaaca gaacaatctt tttgatgaca gcagaataat atcctatctt cctaatataa
    19741 caatagaaac agacaaaata aaaactataa tgattaatga ggttgtccca actaacacca
    19801 atgacgattt ttatagtgta gataaagatg ctgattattt aaaaactact attcctttat
    19861 ttgacagtgc aggaataaaa gtatctaaca taaatgatat cctagatatg ggtatctata
    19921 taactaatgc agtaaaactt cctaaatcag aatatacaat aactcgtgat acaattaaat
    19981 tacatatgcc tattttagaa gaagaaatta aattatttaa aaacctagag gtagtaatgt
    20041 taatgggaga tgttgcaaaa aaatctttta atatgattac taaaaagcat ataaaaaaga
    20101 atgtcatccc ttccatttca acatataaac ttcgtaataa tgaactttat tatgataatt
    20161 taagagtatt tccatcatat attatgactg gtggaaacat actgattgaa aaatcaaaat
    20221 ttgaaatggc atcagaagat ataaaaaaaa tgttcgagat tataaacggt tgatattaca
    20281 aaataataaa aactaatata aaataaaaag tgcctcaaaa acttctattt atgaggcact
    20341 tttattttat attagtgtaa ctatatacta ttaacataat ttattattta tattgttctt
    20401 tttaatctct attccattta gtctaggtaa cattactctt tacaagatta ctttatattt
    20461 cttttatatc tctaacgttt ttatttaata aaacaattat taatacaagt caaatatatc
    20521 tatttatcta aaaatatatc ttttacagta atatctggat ggtgatactt cttcctattc
    20581 gttaaaaaag atttttttac attaatagcc tcttttggac accagtgaaa acaagctagg
    20641 catctttcac actcattacc ccactttggc tttttatcaa ctatacttat attctctaca
    20701 ggacaaaccc ttttacatgt cccacatcca ttacatttat tttctgcaaa aaatttagta
    20761 gttgcaattt gcatattctt acctactaaa cgaagtacaa cactatctat atgtgacttt
    20821 aaattgtttt ttccttctat atttccaatc tccaagttat tgatacattt tgcaatctca
    20881 gctaccctat tagtctgatt tttcaatctt tcttcttcaa catctggagg tgttatcaaa
    20941 gcatttccag gcatattgat taaaaaacca gcagataatt ttttccccct ttttttaagt
    21001 aacttatcta tagtaaataa actatgtcca gctaccccat tacaagtggc aatggcatat
    21061 atatgagtat taatattaaa atttaatttc tctacaaaag actttactat atcaggaaca
    21121 tcagcaaaat aaacaggaaa aacaattacc aatatatctt catttatgtc tatattattt
    21181 tcttttctat gtatagctaa gggtataact tctttacttc catttaacgt ttcttttaaa
    21241 atatttgaaa ctactagcga atttcctgtt ccagaaaaat aataaatctt actattcatt
    21301 cttatccctc tttctatatt tctacatcat caatagttaa aaagtctctt aaaataggat
    21361 ttatatttac tttccaattc tatccaaaat atcagtaaac aaaggcaatt cttcatcaga
    21421 gatagattta aatgtctctg ttgcatatat acttgcaatt tcatttaatg gttcttctaa
    21481 ttctctacct aaatctgtta ggaatatttg aaacttacgt ctatctttaa catctacttt
    21541 acggacaatt aatcccttat ttttcataac atctaaaatt cttgttgtat tagatttatc
    21601 cttacatgat ttttcacata aatctatctg agaaatccca tcttgctccc atagaaacct
    21661 taataacgca taatggtcag ttgttatatc atatccattt tgtttaaaca tcttttttaa
    21721 atcatctttt acttttaacg aagtacttac taacttagtt gtagcttcat atagtttctc
    21781 aaaatctttc attttacctc catttaaata atcccttgct cctcccatct atcaattatt
    21841 cccttatatt gctcaatatc tttaaaagcc attggcttta aaacagcatt tatagcaata
    21901 aaattataga tttgagaaag cacaggtgga tttttctttt cctctatgca ataaataaat
    21961 tttttagata ccttttgtat ctttgattct actttttcag ttataggatt ttgattaaat
    22021 ccagttttta tagataaact accacaaaca taacctcccg aatctaagac catggatttt
    22081 ataaaagaaa ttgcttttga accacgtgca ttttcatatg tagtaactgc tatacatggc
    22141 ttacgataca ataatctttc aagcatcata tgtgctctat ctgtaaacac cttgaaaagt
    22201 ccagttacac tacttccata agttgggcta ccaaatataa caccatcact cttagtaatt
    22261 atatcatgaa tatactctac tttatcattt tgattgatac acttgcccat tttataacaa
    22321 tttaaacaac caatacaata tgatggattt acctcactta aatcaaaata attaattttt
    22381 acatctggta ttaatctttc tatatcttta tacaagtaag ttaaaacttt tgatgtagct
    22441 ccattttttc tagggcttcc atttataact gtaattatca tttctatatc tcctttttag
    22501 ttgttatatc aacaataagt atatcaacta taattttagc tgtcaatata aaccatttat
    22561 ttaaagctat acttacacag cttaaataaa agttttattt aactacatgt gcttatttaa
    22621 tcaactataa atgaattatt tttaataatt gacaaactaa attaatgata atatacttta
    22681 tgtattacaa atgtaagtag aaggaggtat agtatgaaaa agttaccaca tatttcagaa
    22741 gcagaatatc aagttatgaa aattatttgg aaatatgctc ctatcagcac tactgaagta
    22801 attgaaaagt tggtagaaac aagtacatgg agtcctaaaa caattcaaac aatgttattg
    22861 agattagtta aaaaaggagc tttaacatat gaaaaaaata gcagagtatt cgtttatact
    22921 ccacttgtta aagaagaaga atacgtagct acagaaagta gttctttttt aaatcgtttt
    22981 tataatggta ctcttaattc tatggtacta aactttttag aaaatgataa actatctgaa
    23041 gatgatattg aggaactgag agaaatctta aataaaagga cgactaaggg ggataaataa
    23101 atatgtatgc cccattcttt atacgatttt tactcagtac attaacctta tcaatattaa
    23161 ttgctgtaat tttacttgcc aaaaaagtat ttaaaaaaca tatttctgca aagtaccaat
    23221 acaatattgg atttatattg cttataatgt taataattcc atttgtccct cttaaatata
    23281 ttaatcttgg aaatgtattt aactatttat atacttttaa tcaacttgga agcagtacta
    23341 ataggacttc tatgataaaa aatagtccaa attctataat taataactca aatgcattaa
    23401 acgatttttc agtatcccta aaccaagtta attttgatag tttaaatatt ttattactaa
    23461 tatcatggat aattggaatt ttaatcatga tagctattac tattcattgc aacattaaaa
    23521 taaaaaatat gaaaaaatca atacaaattt taagagatga ggagatagta tttatatttg
    23581 aaaagtgtaa aaaagatatt ggtataaata aggattttat actatgcaaa tcacctatta
    23641 taaatacacc tattacattt ggatacttta aaccacacat tatattacca gataagtcta
    23701 tatctaaatt atctttaaaa gatataaaat atatcttact tcatgaatta caacacttta
    23761 aaaataaaga tatactgata aattatataa tgtgcttcct tcaaatatta tattggttta
    23821 atcctcttgt atggtatgca tttaaagaaa tgcgtataga ccgtgaaatt gcatgtgata
    23881 tttcagtttt aaaaaagctt gataaagata gtcatataga atatggacaa actctaataa
    23941 actttgtgga taatatttca agaccatcaa atcttacact tacatcagat attggtgggt
    24001 caaaaaaaca aataaaaaag cgactattga agataatgga ttttcataat gaatcaaata
    24061 cgttaaaatt caaaagcatt ttaattttta cattgatagc tttaattgta tttggaaata
    24121 ttcccaaact atctattatg gcagatatca atgaaaaata tagacctaca aatgaacaaa
    24181 ctgtgtacga ggacttaagt cagtatttta aaggttttga tggaagtttt gttatgtatg
    24241 atttaaaatc caagcaatat catatttaca ataaagaaaa aagtaaaaca agaatttctc
    24301 caaattctac ttataagatt tatagtgcct tattgggatt ggaaaatggg ataataacaa
    24361 gaaataattc acatatccct tgggatggaa agcatcattc taatagttct tggaatgaag
    24421 accaagactt attttctgct atggaaaact ccgtaaactg gtactttcag actctagata
    24481 aaaaaacagg gctaaaaaac atacaaactt atcttaggca aattggatat ggcaattatg
    24541 atgtttcagg tggtatctca aacttttggc tagaatcttc attaaaaata tcacctattg
    24601 aacaggtaga attacttgaa agtttttata caaataagtt taaatttgaa gataaaaata
    24661 tagaaacagt aaaagattca ttgttaatat caaaaaagga tggggtatca ctttttggta
    24721 aaacaggcac tgccaatata aatggaaaag atgtaaatgg ttggtttata ggttatgtgg
    24781 aaaaaaatga aaatacttat ttctttgcta caaatattca aagcaaagat tattctaatg
    24841 gtagttcagc atctaagata acattatcca tattaaatga caaaaaaatc tataatcctt
    24901 aatatattta ctgtacattc taaaaaattc acataatttt catattaatt atcaattaaa
    24961 ttcatataca aggcttatta tataaacata gaagatagta aaactagctt aaaaacataa
    25021 tataataccc tatacaaata gtggcaactc tccctcacat cctcaattgt tgccactatt
    25081 taattttttg tatgaagtta aatatatttt catcatttca acaatttggg tgcaatacaa
    25141 tctctatttt gaaacaacct ctttaattgt tttagagttt atgaattatt tctcatactt
    25201 taaaaagatt gaattactct tgataatctt cttttgtagt cattacatat cacctactac
    25261 tcaacacata ttctttgacc tatttttaca tccttgttta tatccaaaat atttaccttc
    25321 ttaccatctg gaaacaatac cattgttaaa gtagactttc ctaattcatg tattctcttt
    25381 aaatcaacat taacaagaac atctccctgt ttaaccatat ctccttgttt aacagcaata
    25441 tcaaagccat ctccatttaa tgcaactgta tcaattccaa catgaattaa tacttctcca
    25501 ttagttcctt taatcccaaa tgcatgtcca cttgaaacca ctgtttctaa tttacctgaa
    25561 ataggagaaa caatttttcc atctataata tcaatagcaa aaccatcacc taacattttt
    25621 ttagaaaatg tttcatctgg tacattttct aatgcaacaa ttttcccctt agctggagac
    25681 acaaagtttt cattattttc attaaacact aattcttcgt cttcatcttc atttatagct
    25741 tctttaggca ctccaaataa acatgttaca ataaagcaga atgcaaccac aaatattgat
    25801 gctaataagt atgtgattaa attatatcca tcatagatat acattaaata agaaggtaaa
    25861 actgctaatc catatgaatt agattgaata tgtaaaattg ataatattgc tgctcctaaa
    25921 cctgaagatg cacacataat aatcaaaggc tttaaattat atcgaagctg aatcccaaat
    25981 aaaacaggct cacttatacc aaaaagttga gatagcattg ctccaactgc agtttgttta
    26041 acttgattat ttttagattt tttcataaat gctagacatg ttccaatatt cgcaaatcca
    26101 tacattgcac aaagagtaat aagggcatta aatcctgtat ttgctaatag tgatgtttca
    26161 atcattacat aagtatgatg taagccagta ataacagcta atggatatgt aaatccaata
    26221 ataaatcctc caattcctaa aggcaaatca atcaataagc tgatgatatc aaccattttt
    26281 aattctattg tatgcattat agggcctaca cctaacaaaa taactaacat tgttattagc
    26341 ataacaaaaa atggtgtgaa aataaggtct aatacattag gcatatgttt acggaaccat
    26401 ttttcaagat tagctccaat aaatgctgta acaatagctg ttaaaacact tccttgacaa
    26461 cctacaatag gaataaatcc aaatgccata attgcttcaa tttcatttga tgggtcagca
    26521 acagaatatg cattaggaag tattggggaa actaacataa gtccaataac aagtccaata
    26581 acaggtgttc ctccaaatac tttaaatgct gaccatacaa taattgctgg taaaaaagca
    26641 aatgctgttt ctgtaagaac cgaaacaagt gtctgaatat acaatggaat atttgcagaa
    26701 gacattccaa ataatccaag tacattatca ttaaacaaac aaccctttaa tcccaagaat
    26761 aatcctgttg ctgcaataac tggaataata ggaacaaata tatctgctag agttctcata
    26821 actttcttaa atccagtttc attattttta actaattcat cttgttcttt ttttgataaa
    26881 gtttttaatc ccattttttc aacttctgca taaactttat taactattcc tgttcctaaa
    26941 ataatttgat actgtccaga agtaaagaaa acccctttaa cctcatcaac tttttcaact
    27001 ttcttatcat taataatttc tctatcttta accatcactc ttaatcttgt ggcacaatgt
    27061 gtaatagaaa taatattatc ttcaccaata attttaataa gctcatttgc tattttatta
    27121 tatttattca tttttctacc tcactttcaa taatactgca tgaaatggtt tcaaacgcac
    27181 tgttttattt aatagattta tatcatctaa attttggaat acaatatcat atttttcttc
    27241 tactcttatt tctacaggtt tatcagaaaa attataataa caatcaataa ctaccaaatc
    27301 taattgcctt ctatacgcaa taatatcatc actttctata tcaataggtt ctattgaacc
    27361 ataaatcaaa caatcagaat actcactatc ttgtctcaac tttatcatct ttttataaaa
    27421 ttctaaaata ctctctgaat tgttaatctg actctgtgca ttaatatctt tataattttc
    27481 aataaccttt aaccatggtg ttgtatctga aaaacctgca ttttctttat ctgcccatgg
    27541 aaatggcgtt cttgcattat ctcggcttct taaattcaca aattctaacg cttcttcttc
    27601 tgagtaacct tcatctattg aacgatagta ttggtcaatg ctagataaat cattaaaatc
    27661 atttatggta tctcttttaa aatttgtcat tcctaattcc tgtccctgat aaataaatgg
    27721 ggttcctctt aaaaagaaat acattgctcc caacattttt actgcatcat cattcctttc
    27781 gtattgcaaa agatactttg ttgtagctct tggttggtca tgattttcaa taaaattagc
    27841 tgcccatcca tgttcctgaa catacatttg actatctaaa atgcattttc ttaaatctgg
    27901 aattgtccat gaaattcttt taaaccactc actaccagaa gcaacatcta agtcagcatg
    27961 tttaaagtca aagaccattg aaaagaaacc atcttctcca ataaaatcac ctaactggtc
    28021 atagctaata cctgctgctt cagcaacagt catacaatca tattttttaa acgtattttc
    28081 ttttaattca taaagcattt tttctattcc aggctgattt ctaactgttt ttgtacattt
    28141 aacaagacca tcaacaccat cactttctaa actttgaaaa cttaaattct ttttgatgaa
    28201 tgtaatagca tcaactctaa atcctgccac tcccatttga gaccaggtat taatcatttg
    28261 gtaaagttca cttcttaata ttggattttc ccaattaagg tcaggttgtt tttttccaaa
    28321 agaatgaaaa taatattcgt ctctattctc taccttttcc caaacacttc caccaaaaac
    28381 agacctccag ttatttggca aatcgtttcc ttttttaaaa atataataat catgataagg
    28441 actttctgga ttttttaatg cttcttgaaa ccaaggatgt tcatcacttg tatgattaag
    28501 aactaaatct aatataattt tgattcctct atctttactt tttttaatta actcttttaa
    28561 atcttccata cttccaaatt cacttgcaat atccaaataa tcactaatat catacccatt
    28621 atcatccata ggtgatttaa aaattggaca taaccacaat gatgtaatac ctaaatcttt
    28681 taaataatct aaacgagaaa taattccctg taaatctcca attccatcat tattactatc
    28741 ttgaaagctt cttggataaa tttgataaac gatttctttt tgccaccatt tcttttccat
    28801 aaattactcc tttttatatg atatttcttc tttttaattc tttataaatt ccattatcga
    28861 atatgtcctc acatatagat gtcgctatat ctttaagttg ttgatgacta tttttcattg
    28921 caattgtaac gtctgatgct tgaaacatga caatgtcatt ttgaccatca ccaaaacata
    28981 tggtttcttt ttgagttact cctaatcttt cttgtaatct cttaattgct ttgcccttat
    29041 gaaaatcttt ttgaataatt tcataatact gactagaaat atctctctgt gctaattcca
    29101 ttttatcttg tagtatatct ttcacttcgt caaatacctt ttcatttgac cataaacaaa
    29161 ttttatgtat atcttgtgac ttatactctt caatgttatt ttcatatgtt attttttctt
    29221 ggatatgttg tttatttata caagaattag ttcctttcaa ctgattcatt gtttcaaata
    29281 tttctttggc tttttgattc atgaaaactt tttcctgact ctcaatcgaa aaagcaactt
    29341 ctctattttt taataaacaa acaacttcct ttatcaatct ttgattaaaa gattgattat
    29401 aaagtaattc accatgatat tgaatgtaat tacctcctcc tgcaatatat ccatcaactc
    29461 ctaatgataa aacatcatct tgtattgtac ccatacttct tccagtacaa ataacaacac
    29521 tgcaatgatt cttttgacat aaacgtatag catgctttgc agattctggg attccatata
    29581 cctcatctct aagcgtacca tctatatcaa gtattaataa tttgtacaat ttcatcttcc
    29641 tttcttacta tcaaaatcat aatagcattc aaaaaaaaag atatcaatat tttgatatct
    29701 tttaaaaaat atgttcattt ttagcacaaa ttttcatttt cggggaatat gttcactttc
    29761 taaatacatc ttgtatttaa tataaagcag ttcaattaaa ataaaataag gtgttgttac
    29821 atcgtagtcc agttgtttat ctacagaaat agatatagat tgaatataca ggttttcatc
    29881 acataatctt gctaaaggat tacttatcat ttttgtaata gaaaccatat aaattccttt
    29941 cattctcatt tctttagcaa aatcaataat tgtagatgca tctccattca atgaaataaa
    30001 gaaaatcata tcatctgttt ttataaaatg attaagtgcc tgaatcatat cataaccata
    30061 aacatcatat ataattttcc ctgttggtaa gaaaatcctt ttcatctctt tagcaactct
    30121 ttcctgagaa taaccttctc caaagacgat tatacgattt gcctttttca ttctttcaaa
    30181 cataatcgaa caatccttct tctctatatc atttatcacc ttattatagc aatccattaa
    30241 ttggtgttta ctttgatttt tatattctac ttctgtttca tttaagcgta taataactct
    30301 taattcacta tatccaggta attgtagttt ttgagcaaaa cgagaaagtg cacttgtaga
    30361 tatatggtat ttgtctgcaa attcatctat agaaagttta atacaatctt ctttatgatt
    30421 aagaatacac tcacagatat acttatcatt tgctgtaaat gaattataat atttattgaa
    30481 aagtccttca attttcattc ttttctcctt ctatactcta tgtaattcat atgaagcaaa
    30541 tctaatagag cataaaatgc tcctacgact tcataagata tagcttgtaa tgtatcaagt
    30601 ttttgagttt caacatacag attatactca caaagtgttg caattgtatt attatcaagc
    30661 ctagttaaag aaatcgtatg tatatgattg ttgataaatt ttgcaatttc aattcctgct
    30721 tttgtttctc cagacaaaga aatgataata aataaatcac ttttttgaaa tgattctctc
    30781 ataaattcaa tttcacctat atcaaataag tcaataacac atttacctac tgaaagaaat
    30841 attctcttta tttcttgagc taaacttttc tgttcattac ctgtcccata aatataaatt
    30901 gtttctgcat catatattat tttacaaata tcttgaaatg atattttttg taattcattc
    30961 attaatagtc gataattctc gcaaacatca tgaaaattat tatcattatt aacaaattct
    31021 agttgattat ttttaagtaa atactttaaa tcagagaatg aatctaatcc aatttttcta
    31081 caaagcctta ataaagtagc tctagaaaca ttacattcat ttgcaatttt ttcacacgaa
    31141 aaattacggt attctctttg acaaatcaat atttgtttaa taataatttt atcattttca
    31201 tttaaattat cataattttg gttaactaac gattctaatc tcattttttc cactccctaa
    31261 atattatttt aatatacatt agttgctttt tctagggaag agttttaaat aataaggata
    31321 aaataatcat ttgattttat ttttattaaa ataaaaataa cctgctaaaa gcaggcacca
    31381 aaaagtactt atcctttcga acatgtactt tagatattgc tttcttcgga agcttcatct
    31441 cttcatactc taaaatagag ttttttctaa ctcaaaagta ttttccatgg tagaaattac
    31501 tttaattcat ctgccattta ttttttatct tatttaataa ttactactac ttgaatattt
    31561 acttctttaa gtaaatttca tagttaacaa agctaaaatt attattatag atgttaaaat
    31621 acaaaattgt aagtattgtt ataaatatgc ttacagctac aaatatacat gatatattaa
    31681 aaagaagctg taaattatta tcatacatac aagcaactac gcctctatta atccatgtta
    31741 atttttatta tttaatacaa ggagttccat gagacataca tatccattca tattttaaat
    31801 tatccaattc tcttatagat ttcatcatct ttttataatc ccaattccat ggattaggat
    31861 aaggaactat atttccattc ttatttttga ctaaatcacc tgcaaataat acatcttcaa
    31921 aaatcatgca gacatgccct ggagtatgac caggagtatg gataattttt atattcccaa
    31981 tctgcatatt ctctccataa ggttcaacat ccttaatgag ccttctggaa attgcctttc
    32041 caataaactt cttaaaccca ggtctatcct tctctcctat aataaaaggt atatcctcta
    32101 catgagccca tacttttgct cctgtttttt ctttcaataa tttaatatta gatatatggt
    32161 caacatcatg atgagttaat aaaatatgct taatatctgt aagtttaatg ccaagtgttt
    32221 ttaattcttt aattatattt ttccccataa aaggaagacc tgtgtctata agaataattt
    32281 ctttatcaaa cacaataaaa caatttgagc cacgagtact atctaacatt aatacatttt
    32341 cttttatctt cataaataaa ttccccttat ttggtatttt acctatataa ttaattaata
    32401 aagttataaa tagttataat acttgatatt aattttattt tttattaaag tatactatag
    32461 gtatacaaat attatattta cataaatata ccatcagtat acttttttat caatagaaag
    32521 gaataattta tatgtcagaa ttatcaaaaa gagataaaga aaaaatacaa agagaaaatg
    32581 agattattga taaggcagaa aaattatttt gtcttaatgg atttgataat acaactatga
    32641 atgaattagc aaaagaagtt gaatatacta aacgcaccat atacaagtac ttttcatgta
    32701 aagaagattt attctttgct gttgtcttaa gaggatataa aagactttgg gataatgtta
    32761 aaatagaaag tgcaaaaggc aaaacagggt ttgaaaaaat aaaactttca tactttgctt
    32821 ttcataaatt ttactctgta gaaccttctc tattatgcct tatgggaatg attggtattg
    32881 taaactctga aaaatcggat actgatatgc tgtttaaaga aaagtttttt tcttttaata
    32941 aatttatgtt tgatgaaata caaggaatgt ttgaaattgg aaaaaatgat agaagtatta
    33001 ggcatgatat tgaaatacct actcttatgt attcatcaat atttacactt acaggatttt
    33061 ttaatttact atctgtaaca ggaaaatcat acctgaacaa ctttaatata gatgaggaaa
    33121 aatttattga aacaactctt gctcttttaa tagattcaat taaagcataa acctaagttt
    33181 ataaattaaa gtttatatac ttaaatgata attttttcta tctctaatat gaaaaatatt
    33241 aaacataaaa ctaataactt agttaatgga ataactaaat tactgaaagg attaaaagta
    33301 aatggctaaa atacttcttt tagaagataa tgatacaatt acatttggaa taaaaacttc
    33361 ccttgaaaag aaaaatcaca ctgtatgttg ttataatacg attgctgaag ctaaaaaaaa
    33421 ttttgataaa actattgatt taattctgct tgatttaaat ctacctgatg gtattggata
    33481 cgagttttgc caatatgtta aaagtataga agatactcca gtgatatttt tgacagtccg
    33541 tgatgatgaa aaagacatta tacgtggatt agatatgggt gcagatgact atgtggttaa
    33601 accgtttttg ctctcagtac ttcaatccag aattaatgca gttcttagaa gaactcattc
    33661 aaatactaac aatactatta catgtgaaaa tataagcttg aataaggttg aagtcaaagc
    33721 atatttagat aatgttgaag tacctttgac agcaggagaa tataaacttc ttctaagtct
    33781 tatggaaaat aaaaatagag ctatgactcg tacaaagtta ctagaaatgc tctgggatgt
    33841 agatggtaat ttcgtaaatg ataacactct tactgttaca ataaaacgtc ttagagaaaa
    33901 attaaaaaat ccaccttgta taaaaacatt aagaggcata ggatatagaa tggaggacaa
    33961 ctagtgaaaa ctccaagaaa tatcaacagt acagttgttt ttataatatt ctgcttaatt
    34021 gtaacttttt tattgagtgt aattctaaca actataactt cttttaatga atacaatgta
    34081 ctgtctaagg ctcttggtgc atcatatgag tacaatccta agtctacaga aagtatacta
    34141 aaatcactga aaaattctag tgataaggat tttaaaacag gaaatgaaat tttatcaaaa
    34201 catggatatt ctaaaagtac attttggagt aaaaactttt tttattttct agtgatatcg
    34261 tttggtataa tttcaacaat ggctacagtt gtatatataa tcaaacatag aacaaataag
    34321 caaaaactga gtagaataaa caatcttact caatacttag aagaagtgaa tttaggaaaa
    34381 gagtctgtac ttttacgatg tgaagatgaa ttttctcacc ttgaagatga gatatataaa
    34441 acagttgggg aacttcgttg ttcaaaagaa tttgctttaa aagaaagaca aactctttca
    34501 gataaccttg cagatattgc tcatcaactt aagactccaa ttacctctat gtcacttatg
    34561 gcacaattac tttcagaaaa ttgtccagat gaagaaatag attatattaa cagacttaca
    34621 aatcaaatct caagacttga gagactagtt tcatctttac ttacattatc aaaattggat
    34681 gcaagtactt taatatttga aagcaaactt atagatgtac attctcttct tacttatgca
    34741 attgagccta tagaaggttc tttaagagaa aaacatcaga catttactat cgaggcttct
    34801 ccttcggtag aatttgaaat cgatattaat tggactttag aagctctttt aaatattctt
    34861 aaaaattgta gtgagcatat agaagatggt ggatatatag ttgcttatta tagtcaaaat
    34921 cctctatatg ttgagattat catagaagat aatggtaagg ggtttgctca ggaagaactt
    34981 ccatatattt ttaatagatt ttatcgtggt aaaaatgcta gtaaggacag tattggtata
    35041 gggcttgctc tatcaaagtc tattatagaa cgtcaaaatg gtactctaca tgcagaaaat
    35101 tgccataatg gtggtgcaag attcataatt aaattttaca cataaaatgc tggattatag
    35161 gtaaaatacc tataatccag ctatatctat attctatgtt atctattgtt ttagtaatat
    35221 acttctattt acatttaagg ttttattaaa caaacaactt aactatccct atacttgtaa
    35281 gtagtcttaa atcgaaacct ttaaataagt tcaaatagat tttgtttata ttgtttcatt
    35341 ttttatagat tctactatat tttcattctt caactgcttg tatcctgtca tatatgatgc
    35401 tattacaata aaaagtatca atatagtaaa acctattatt gtagatatag gtgcaaatgg
    35461 taaatactct ataaaatata tttcatttat acgtagaaaa gcatacacta ttcctatttg
    35521 cacaggaata ctaagtagta atggtgtaat tcctaagaat aaaccttcca aaataagcat
    35581 cttttttatg ccttctggag ataatccaac agaacgcaac atagcaaact cttgtcgtct
    35641 acttctaata tttcctgaca ctgtagcaag tacatttgat aatccaatca tagccaataa
    35701 acctgtaata aagcctacta ttaaattcat ggtctttatt ccactagcct tttgtttttc
    35761 actttctaaa acatcctcaa tactataatc tcctgaacca tagtctttat tataaatgga
    35821 ctctaatttt tctcttactc taggaatgtc ctcacgatta tttactttaa atactcctat
    35881 cagcctattt gaacgtaacc tacttacttc atcataattt ttagatattt cctgcaccat
    35941 agacattgga actatttgca ttaatgtata ctttgaacta gtatctacta ttttaggaag
    36001 tgtgcttgta atagctccca ctttcatgtt gtaagtataa tcccctttat cttcgtcata
    36061 tactttttca tttaatttaa tgttatctcc tattgaaaga tttaaataat ctatatatac
    36121 tttatttcta cgtgtactac gattaatatc ttcctcctta ttatatacaa ttgaggttgg
    36181 attttttgta tcataaaata actttgggtc tacagaaatt tgcctacaat attctgtaaa
    36241 actcttatca tcaagcccaa ttatatttga aattatacga aacttgccat cacgttctat
    36301 aggagagtac tttttagatg aagctatatt tttaaatcct ccatttttcg caagttcttt
    36361 tgaagcatct ttttcagtca accaagtgga agcagacatt ttttgataat atagtgaact
    36421 ttttacacct tctgtattat ctaatttttc aatgaaagca gactctacag gttcactatt
    36481 ttccaaataa actgctatat ctctttggtc cttataatta tcacttccat aaacagcttt
    36541 tgctccttct tgatttgcaa ttatatgcaa aaaacatgta agtaataaaa aagataatgt
    36601 taaagatatt gttgcagtac gataagattt tttacgagca cgtagtgcgt ttgatgcaag
    36661 ttctccttct attccaaata tctttcctaa tgaatattta tgcgactttt tgattttaac
    36721 actatctcct tgtcgtattg cttctatagg agatatcttt gatacttttc gtgctggtat
    36781 aagtgcagaa atccagactg ttactattgt aagtaaaact gctggcagaa aagcaggaat
    36841 accataagta aataccactt ctggaacatc agtatgataa ttaactgaat taacataaac
    36901 tataagccta ttacacaata accatcctaa aaataaacct attggtaatg gtataattgt
    36961 cagtaaaaag ccttcaaaca ctacagacct tttaatttgt tttggtgatg caccaacact
    37021 agataatatc cctaattgag taagtctagc atttgcagac agtgaaaacg cgttgtaaat
    37081 caccattaca aacacggcta ctgtaaaaat tgctattgta agatacataa taggagttga
    37141 aaattttgat atactactca tagatgcttt ctgttctggt gaaaagacta acatctttaa
    37201 aagataatta ctattgtatt taagattgta ttttccatat tcattagtct tccatcccaa
    37261 tgatttagca agttttggaa gttccttata cgtgtctctt atatttttaa atcgcaagta
    37321 cacagttata ctatcattag gttttatact tgttttatca agatatccaa gagctgtata
    37381 tgcaggtatt actgatgaag tagaaacatc tacctttcct actattgtca atgtagcctc
    37441 tgaaattttt ttaaaacttt cccctttcga ttttggggct atgggttcaa gaggctttcc
    37501 atttaggatt ctatctccta ttgggagagt aattttatca ccaattttta cacttggatt
    37561 attctcaaaa tattgttttg ataatgcaat ttcattagca ttatttggtc gacgaccctc
    37621 tattatagta tccttttctg gcatagaatt ccaatactct gatgtagcat ttctataaat
    37681 tatataatct cttttctcat cattaatttt gcctacatac cattgtcctt ttaccattac
    37741 atcatcaact gaagaaaaat tttcaattaa tggtaagtca cttccataag tatagtcaaa
    37801 caattcacca tgccagttac cttctttctc aattgaaagt tttataaaat ctgtccacat
    37861 ggtatacaac agtccacaca aagaagacat catggttgta gtaagtaata tagctaacat
    37921 tatagctata ctactacgtt tattgcgtcg tacaaagtct aaggtgtatt cttgaagaat
    37981 attcatttct tcaccacctt ttcagagaca atcactccat cttccatagt tacaatacga
    38041 tttgcctcta gtgctatttt ctcatcatga gtaataagaa gtattgtctg attaaatcgt
    38101 ttatttgata agtttaacaa gtctacaatt tcttctgaat tttttctatc aagattacca
    38161 gttggctcat ctgctaataa aatagctggt ctatagatta aacttctagc tatagatacc
    38221 ctttgttgtt gtccacctga aagttgactt ggaagatggt ttaatctatc agataatccc
    38281 aatatagata caatctcaga gaatctatcc tcatccacct ttcttttatc aagtaacatg
    38341 ggtaataata tattttttct aacatcaagg gtaggtataa gattataaaa ttgataaata
    38401 agacctactt ttctacgtct aaaaactgca agagcttctt cctttaaact tgatatttct
    38461 gtatcttcaa cataaatctt tccagatgta ggtctatcta ctcccccaag cagatgtaac
    38521 aatgtagatt tgccagaacc agatgctcct acaatggata caaaatcacc tttttgaact
    38581 gataaattta cattattaag ggctgtcact ctggtttgat ttgaaccata tatttttgtg
    38641 agattttcac attttaatat ttccatatac aatcatctcc tttatggtta aattttagca
    38701 acctaaaatg acattatagt gacatgaata caaatataaa ttactattaa aaatattgtt
    38761 tagtttttgc acattattta aaattataat ataaaacaaa aactatgcaa tatattttat
    38821 ttatactttt aatatattac atagtcttta aaaggtatta actttaaatc tataggtatt
    38881 caatttaata atcaatagca agtattttat gtactaaagg agttttgctc tatatagact
    38941 caatttagta aaattattta ataaatatta cagactatag gtaaataata tttacaaata
    39001 aaattaagtt tattccctta cactataata tttcttaatt atattaattg caatttcctt
    39061 tacctttggc cctgtaattt ctttattgga atcatctgat ttaatagcaa aataataggt
    39121 gtcaccttca cgctctagca taccaacaaa ccatgcatcc acacgtttat ttttttcatc
    39181 aaatcctgtt ccagtttttc catatacatt tatgttttta tcatttacat ctattttcat
    39241 aatatctctt aaaatattaa tatgctcttt tttaaagttt gtatctccct caaatatttt
    39301 tgccattgtt tgcacttgtt cttttggaga tatttgtaat gaagattcta accaaaatcc
    39361 atttaaatgt ccctttccat tttttaaatc tccttcccat tcgctaatat cgcaattccc
    39421 atactttaaa tcatctaaaa tgttttggac agatttagca tcaactttat ctatcaattt
    39481 tttgtagtac caaacacaag actccttaaa tgcctcttct aaactaagat ttttattcca
    39541 atttttattt ggatactctg taccatcata tcccataact gattcttttg agtttattac
    39601 ccctttttct aaaccaatta aagttgaaac tattttaaaa gtagaacatg gtgaccttct
    39661 tgtttcaatt aattctttgt tatatatatt atattcttta tttttagtat tataaaaaat
    39721 agctcctcca ctaatacctt caaaacaatc actatagtca accatattaa ttttttcttt
    39781 tttgttttta ttattgtttt caattttgga tttatttaat tcagacttat ttaattcaga
    39841 ttttatataa ttcttatcag ttttttgatt tatatcgtta tgttttttcc ctacataata
    39901 cattaccata actacaccaa ccaataagat agctatccaa ataaaattct tttttctttt
    39961 cataatttat atcatctccc atatttaaat ttataaccta ctattaaaat agtagacatt
    40021 atttgtaata tactccaatc taggttctac ttataagaaa caagactaaa tgaatagctg
    40081 tatctcttat attttcttca cctagtacaa ttatgttgat taaacctatt gcgaagataa
    40141 ttaagtaact attaataaca tcctgtcctc cttttaattt gtgatataat aattgattcc
    40201 acatttaaaa cttactattg taatatttaa atattacaat agtaagtttt aaatgtcaag
    40261 ttagtttggg aaagttattt taattgtata attgttatta catttataaa atatggcatg
    40321 tttaaaatta ttttgctatt gggaggatta agataagcag tattataaaa gctataaatt
    40381 ataaaattta atatataatt tatagctttt acttttacaa atattgtttt tattgattta
    40441 ttaatgaatt ttattgacag gttatattta tataagtaaa cacttacata ataagtatat
    40501 aatctacata tctataagtt gagtcaattc accagtatat gtcaacttaa gtttatgcat
    40561 agctcgtgta caagcaatat ataacaaact gcggtcataa tcactataat atgttttatt
    40621 atttactgaa ggtacaataa cttcatcaaa ctctaaacct tttgacattt taatagaagt
    40681 tatggatact ccttttgtaa agctagaact ttcagaagat attaagtgaa cactatattt
    40741 ttctcttaac gcatcatata cagctttagc gtcattatct gtctttaata taataccaag
    40801 agtcgcatta tcactctttt taaattcttc aatttctatt tttatcttat ttatttcatc
    40861 ttgctcgtta ttacatttta ataaagctgg ttcttcacca tgtcgttcaa taggctctaa
    40921 tgaagatatg tcttgaatct ttttggcgaa attaataatc tcaaaagtag aacggtaact
    40981 cttattcaac ataactagtt ccccatcatt atatagctgt ttcatatcat ctagtgtatg
    41041 agtgtgatta ggattaacca attgtccaaa atctccaaga atagtctttt tacacttaaa
    41101 aagtaagttt attacagcat attgaatagg tgtgtaatcc tgcatttcat caataacaac
    41161 atgtcttata atcttatctt cttgtatacc ttcatatgca gcatgaatat atataaatgg
    41221 gtatacatct gaccattcaa gagttttttt tgttgccatc acaaacatgt ttgatatatt
    41281 catttgttta aagaaatcct tatatagtgt aagagtattt tttattgtca acattccatt
    41341 caaactttta agtatacttt ttacctttgg taaatcttct tccataatat tatctgattc
    41401 aaatttataa tgaatgtctt cagctacctt ctctaatctc ttcttaactg gatatctgtt
    41461 atatgcctca aatctactct gtatccagtc acttttagct gtaaaacttc caaaagtgta
    41521 gtcttttgga ataaaaatct tatttggcat ttgttttata taatcatcaa taagttttac
    41581 aaaatcaagt gtagatttaa aacgcactct ttcagaccat ttagcatcat ttatttctaa
    41641 tgggtctttc tcactttcaa aattaataac tctatctaat tgtacctctg ctatattttc
    41701 aaaacttaat tcacataatg gctcttctcc aagctcagga aggacattgg aaatatagtc
    41761 tccaaagact ttattaggtg ataggataat tacattattt gctgatattt tatctttaaa
    41821 tcgataaaga agaaaagcaa ttctgtgtaa tgctatagat gttttcccag aaccagctac
    41881 cccttgaata atcagtgtat cagctttatc atttctaata atttgatttt gttccttttg
    41941 tattgttgaa atgattgact tcattttttc atctgaagtg tgactaagtt ctttttgaag
    42001 aatatcatct tgtatattaa tggaactttc tagtgcatat tctaattttc catttttaat
    42061 tttaaattgt cgttttcttg ttatttctcc atccacccat cctattggag catcatatcc
    42121 agcttttccg atttcataat cataaaacat actggcaatt ggagaacgcc agtcaagtat
    42181 tataagctca tcttcataat caaatgcaaa tctaccaata taatacttag attcatcatc
    42241 catatctttc agcctaaaat ctattctagc aaagtatgga gaatctttga ttttattaat
    42301 cttatctcga acttttacca taaaagcccc atagttatct atttgtttta gtccttgttc
    42361 attttgaaac atttcatgtg ggtctatttc accacgattt tttaccatat acattttagc
    42421 gtccatataa tccttatcaa gcttgtcaac aagattttca gctttcttaa aagcattgtc
    42481 tagcttgtta tttatctcta ttagatgtgc aatctcatct ggaaaaaaag ttaataactc
    42541 tgcatcatat tcacacatat tatgtttaga attagatgca ataatatcgc aatttactac
    42601 ttgtttattt tcagacattt aaatcctcct ttgttaatta agtacttaca ctgaatcatt
    42661 atacttaatt gtgttttgga gtttttaatc attcttgcta tttaacttaa atgttagctt
    42721 tttagcttta aagttccttt tttaaaaaac taaatctttc tatatttatt accataagta
    42781 cacttacaag caaaatacat taaaagcaga aatgctctta tatataaaat aattcaaaca
    42841 aaaaatatgt ggtaaacttt tcatttaatc tacaattaat tttgtctatt atctctttct
    42901 tctacacaag gaagaataaa tttttgagta ggatatccaa attccacaag caattctgct
    42961 tctgcaaagc ccaacttctt tatcatatct ctatgtcctg tatcagcttt gtcaccctct
    43021 cgaaaggtag taattgaaat atctgtatct atcaagaaat cttcaataac tttcttcaaa
    43081 aacacttgtg caatgtctga attacgatac tgtggatgta ttccaaaaaa atcaatactt
    43141 ctagtttctc tattaaacac catatttgca atagcgacat tattaagttt taatattaaa
    43201 gctctttctt ctaaaatata ctgttctaat tgatatatat attcttcctc ttgcaaattt
    43261 ggaaatccat ctatgactaa tctcactaaa ttcatccata atggtatgtc agacatactt
    43321 gcaagtttaa tttctttttc caactctagt acattctttg aaaataggtt atcttttttc
    43381 aaaacaaatc ttagctgcaa aggataaaat tcttgttctt tcctatattt atttggagat
    43441 tttttataca tagctttaaa tatagaagtg aatgcctgtt gactttcata tccagctatc
    43501 aatgcaattt caataattgg tttctttgaa aataccaata attttgctgc ttctgtcaac
    43561 tttctccgct ttatgtaatc atgcattgtt agaccaacag aagttgtgaa agttctatgc
    43621 aggtgatatt ttgagtaatg aacagcatta gcaactatat ctaaatccag cttttctgat
    43681 aaatgctcct ctatatagtc aatagcagtt atgacattaa ttactttgta agacattgca
    43741 gcttccctcc ttttatccat gcaacattat atcaattatt acttagttat atttaatgaa
    43801 tcttgctatt ttataaacaa atttgttgtt taagttttca attcataaat ttaatatatt
    43861 actttttact ttctgattaa agactctaat cctttaattt tcaataactt ttcatttagt
    43921 aaatctaaaa agttatttat tattttcttc ttattagatt ccttttatat tccttaatta
    43981 aatgataaat atttatatta tacgactaaa aacagctttt tacaattatt taatatgatt
    44041 acaaacttac cctatcttaa agtttaatgg ggtcctgtta tgaatctttt tattttaatt
    44101 taagctcttc atcagtttat tttatatatt gatgtatttg atttcataca aaaaagagtg
    44161 cttcatgaac taaaaagttc atttttaaac actctctttt atgtaaataa atttctattc
    44221 tattttgaaa tattataatt tgacttataa tctgttaata ccaaaaataa taaattgtac
    44281 gtctaagaat agattctaat tagttgctca gtagtaaaat ctgtttttaa ctaatcctgc
    44341 tttgtatgtt gaacatattc tataattgta ccatctggat gtctcaatgt catattatat
    44401 cctgtaggaa cttttttctt atctcttact acaactgctc cattatccaa tagataactt
    44461 ctccattcat caacagaatc aaccataaaa gtagattttg tttctataaa tggtttaaga
    44521 gcatcatcag aaccagataa tagtaaaaca tttcctattt gcgctaattc caacccaact
    44581 tctttatagg aaaaacgcaa agaacatttt tcttttaaaa gattttcata aaaaggtatg
    44641 gctttttcta tatcttgaat ataaaaacgt gttaatatat catatatttt catattttcc
    44701 ccctatatat ctatataata ttacctctaa attataccac tctacaaatt atataacaat
    44761 aaattaagca tagccttttg agcatcttaa gtagacctta acaatatatt ggggttacat
    44821 tagaatacat aaattaaatc accttgatat tatttaggat aaaatttatg tagtagcagt
    44881 aaatgttctt ctaattatca actaacaaac gataatcttt aactttctat ataatgggta
    44941 acatcatttt cgtaagatat tcactagctg gacgattgat atcatttaaa tactggtaat
    45001 atatatcgcc atgaagttta tatccattgc tatttgccca tgaaaataag tcttctaaag
    45061 tagggtcttg taattcataa ggtcctaaat caattgcagt tattattttc tgtgcaggaa
    45121 caatcctttg tagaatctca tttttcccat ctatataagt ggctacagga aaccctactt
    45181 caacatccaa ttttgctaaa tccatgttat gaaaacatac aatcggtgct cctgaagata
    45241 aaattcctct ctcctccaga tatttagaaa ttttttcaaa actttgtttt gaaaactctg
    45301 aaaactctac cataaagtca attgtttttc gtattgccaa aaaacagtac tcagattgtt
    45361 cagctattgt tatattggat attctagtca ttttattcct ccattacttt attttcattt
    45421 ctaatctttt tataccaaat tatacgaaaa tttactgtgt aactaattat ttcatatgaa
    45481 tataaaacat aacctccttc catttcttat atcgaaagta tagcaaaaat aatctcatca
    45541 ttcttgtcac acagtgctct tataatcaga acatacttaa aagacttgcc taatttttag
    45601 gcaagtctaa aagaaatctt tttctataaa aaatttatac aaacaattaa aagatcaaaa
    45661 tatagactaa tataaaacta taaatttact aatctgaacc tcccatggct aaagtcacaa
    45721 gattactgat ttatagaatt tcgcacacta aaatatgtct tactaattcc ccacaggcta
    45781 cccccctagt tcctacgttc ttacataagt tatctattat caaacatcaa aatttaaaca
    45841 agtttcaggt tcatcttcta ttcttgctat tttaaactta catgcaatta atactaaaga
    45901 tactattgga tttataatat ttaataaagc gtatggacca tatgttagtg cagatacacc
    45961 taatgttgct gttatatacg ccccgcatgt attccatggt actaatggag aggttaaagt
    46021 tccactatct tctaaagctc tcgacaacat cttaggatgt atacctctct ttttatattc
    46081 cttattatac attcttcctg gtataactat agatagatat tgttcacctg ctattatatt
    46141 ggtaattgta caagttacca tagtacataa tactgttcca aatacaccct ttgctaatct
    46201 tagtaaagaa gatgctacta cttcaagcat acctgttttt tctaaaatac ctccaagaga
    46261 taaagcgcat attgttaagg atacagtgga catcatattc ataatcccac ctttagataa
    46321 taaagcatct acctcctgta tttgtgttga cgaaacatat ccattttgtg ctgcatctaa
    46381 tatcgtattc atagattctc cttgaaaaac tactgcaaac aatactccca atacagcacc
    46441 tactataagc cctggtatag caggaacttt aaatacaacc aatccaatta ctattgctgg
    46501 agctaataat aatactggtg ataaagtatt aaatgtagaa tctaatgtag acctaattaa
    46561 ttctatttgt tttatatcta gtgcttgacc tgaatacttc attccaatta caccataaat
    46621 tactaaacaa attaataatg atggtatagt cgaatatagc atatatttta tatgtgtaaa
    46681 caaatctgtt cctgccattg ctggagctaa gttagtcgtt tcagataaag gtgatagttt
    46741 atctccaaag taagctccag atattataga acctacgatt aatccaattg gcatacctaa
    46801 actttgacca atgcctaaaa gagctactcc tactgtaccc attgtgctcc aagatgaacc
    46861 tgtcgcaaga gatactattg cacagactaa tgttgatgct actaaaaaaa tatttggtga
    46921 taaaattttt agtccccagt aaatcataca tggaactact ccacttacta tccaagttcc
    46981 tataagtacg cctattataa ttatgattaa gattgcttgc attgccatct ttatactatt
    47041 aaacatactt aattctaact ctttccattt aaatccaagt ctatatacag ccacaagtcc
    47101 tgctactgct gctgctccta ttagtggtac atgaggtgat gaaccataca ctaatactcc
    47161 tagacttaaa aataatatca aacaagtaat tggtatcatt gcatcaaaca gacttgcttt
    47221 tctacattct cgctctttca taaaatagtt tcctcctaat tatttaaaca ctacactata
    47281 attttttttg aaacatattt gctcgtacta gatgacatga tacccccata agttaataaa
    47341 aattctaaat tgtcacctaa tttataatca tattttgtgt tacttacatc tagtatcatg
    47401 tggtctgaac ttgcccctaa tatttttata tcagtgtcta ttggaattaa agagtcaata
    47461 tttatatctt gtttacctat agctatgatt gcccttttta agattccctt atcttcataa
    47521 acaggtttat ttctaaaggc atcaactcct atttctccaa taggaactga tggtttttct
    47581 ttacattcaa ctacttggca tattaattta aatgcatctt gatatgttcc atttatattt
    47641 tcaccgtatg ccgtttctct accaagtaga atggactcgc ctattctcaa attagtaata
    47701 ccttctggca tattattatt tatcaacaga tgaatagaac ttgaattacc tccagatact
    47761 atttctagat ttattccaaa tttttcttcc attctttttg ctatacttac cagtctgcct
    47821 atattttccc tagatggtat aatagctcca taacaggtta agtttgtagc tataccaatt
    47881 atttcaatat tttttaatct tactatctct cttacattac taaaaaagtc ttcttcataa
    47941 aagtatcctt ctcgtaaatc gcctaagtca aacataagaa ctatcttatg aataatattt
    48001 ttactttcac ataattcatt taatttcttt attgttccta attctgagtt aaaactaata
    48061 tcgcaataat tgataacttc ttctaattca cttaacattg ggattctaat caatatctta
    48121 ggtagattta tatcttgtaa attttttaag ttttgtattc ttgaatctgc tatatggtct
    48181 attccttcac tacatatttc ttctactatt tctttttgtg cacaaaatga tttagtaaca
    48241 aaagatatct taatatttct ttcatgacac atctgcgaaa ttaattttgc attatgcttt
    48301 aacttttcta tatctatttc taacctagga tacataatct ttcctcctca aaattcaaac
    48361 tttttaacag gagcttaaga gatgcttctt catatttttt agataaaaca cccatagatg
    48421 ccataatata gtcattatct attaatatct ttgttttttc ctttggaaat agtaattcat
    48481 tatttttatt gtatttgagc atattatcta atatttcaat tctactaggg agcctagtaa
    48541 gtgctccccc tgtaccaact atatatctta cttctgttaa atctttccct tctgcaattt
    48601 tgacttttcc agttgttcca tagatatttc ttatttttcc tgaatgtctt agcatagctt
    48661 taacaacagc ttcttttgtc aacctctcaa caaataatat ttcttctttt gattttggta
    48721 ttggaggata attctctatt actgcatcaa tatctataga tagttcactt tgtaagtttt
    48781 ctttacctat aacttctact atattcttca tatttacata tacacctaaa tccccctcaa
    48841 cacttctctt tgcagtaggt tcaggattaa ccaaaatttt attaatatag tcacttccat
    48901 ctgtaacaga atgtacatca gtagttgctc cacctacatc cagagttaat aaatctccaa
    48961 tatttttata taaaagttta gaagcttcca ttacagctcc tggggttgga gtaatatttt
    49021 cattaaccat ttctttaatg tatttcattc ctggtgctgt agttatgtgt tcttcaaata
    49081 cactttgaat gattcttctt gtaggttcta tattcaataa atctatttta gggtatacat
    49141 tttcagttat ataaagctta taattagtat cttcaaatat taaccttatt tcctcttgat
    49201 tttcaacatt accagcatat ataactggta tgcctagatt catagaagct atcatttctg
    49261 cattataaat agctgtatct ctttctccat aatcgactcc accagctatt aaaattatgt
    49321 ttgggtttat ttcttttatt ttatttaaat cagttctttt aattctacct gatgttattt
    49381 gacgaataac agctccagcc cctaaagctg cttctttagc tgcttttact gtcatatcat
    49441 agactaatcc atgaacagtc atttttaatc caccagctgc actagaagtt gcaaacatat
    49501 cattatactc tatatcatct acagataatt tattagctaa atctttaata gcacctgata
    49561 agccatttct tacatcgcca ccttcaaaca ctgttgttgg agcttgacct tgacccaaaa
    49621 atataggatt gttagtatta atatcgtgga aagcatttac cactgtagta gtacttccaa
    49681 tttcagctac caacacatct atttttaaag aaaccttgct attttccatt atttattact
    49741 catttctctt cttttttcaa ctaagaaagt ggctacatgt atgcctttac tgtttcttcc
    49801 aaatccagca tctatgcctt gttcaacagc tatttcaggt gttacttgtg ttcctccaca
    49861 cgcaatcatt actttatctc ttataccttt ttctacacaa taatcgtgta attttttcat
    49921 gtttttatag tgaatatcat catgacttat tattgtagat gcaagtattg catctgcatt
    49981 cagttcaatc gctgcatcta ccaatttttc tacaggtaca gaagtcccta gatagtggca
    50041 ttctatgcca tatttttcaa tacctccatg ttttatatct attatttctc ttaaaccaac
    50101 tgaatgttca tcttctccaa ccgtagctga aactattttc ataggttttt cttctatatc
    50161 tgctcttatt tcttcatcag ttagtacctt aggttcttct ggtattacta aattgtttaa
    50221 atctatatca aacggaactc ttcctttcac ttctattcta gtcccttcag attcatgcat
    50281 aacttctcta cttatgacct caatttcttg taagttcatt tttttgacaa attccaaagc
    50341 tgcaaactct gctgttcttt tatcagtagg aagcatcatt gttatcatta caattccatc
    50401 tgcacaccat tccatttcag gcttaatttt agttccattt ctgtactcat atgattcttt
    50461 tagtctattt tctacattat caaaatcgtc aagctcatca atatatatta ttttgtctgg
    50521 attttcaaat gtacatccat ctattaacat tgatggatta tctatagcat tttcatcata
    50581 ttgagctaca ttattgtatc caaagtgagc agttacaggt gctaaataat cttcttctct
    50641 ttcataaaca gtaccttcac caactccacc ttttattttt ctagatattc catcaccatt
    50701 tctttctgga taatttccag aatcaacaaa aaatccttgt tccacagatt caaaatatcc
    50761 accaacttca gatatttctt ccatatataa tacagctctt tctttaagtt ctcttgcttt
    50821 gtctcttaat tcacccacat ctttaagttc tatcatatcc ataagcccat ccataccaac
    50881 taaagcttgc tttgctgtat cacatgcttc tatattgtat atatgccaag gtacatttct
    50941 accttcatca ggtgttatag ttgattgtat gtcagctcta gttaatactg atgttaataa
    51001 gtttaataca tgagtaactg ttgcttctct agttgatgat tccatatatt tagtattcat
    51061 ttgagctctc atcttataat cactaaataa ctctctaagt gcaacagcgt aaggtaagtc
    51121 tatttttaaa catggtgctg gaggtgctgt tggtggaact gttgataagc atatattact
    51181 tttatctata cctatttttt ctgaaaatat agagtttaaa gcgtgttgaa ccataagttc
    51241 aggcattacc ttccatgctt ctctagcagt agcattagca ttatgtgctc catcaatttg
    51301 agctatattg gcaaaagcca ttattttctt tgcctcacaa gcatctacaa aagaccttac
    51361 catgtttata ttcctataca gaacattata ttgtgggtct tgatgtgctc cattgactcc
    51421 ttcctcagca aacattactg ctacttcagg accagcaaca ccacttacat atgaatggta
    51481 attaataggt ctaccaactt cttcttctat aaaatcaaga gccttccttt gagctcttac
    51541 ttgttttctt gttattggaa ctccacctat accttgtgga gttccttcta taagcccatc
    51601 aaaatgtgat tgtccagcag ttctgataac cattagatgg tcagctccat gatgagctgc
    51661 cattctcatt cttcttatat catcctcaaa tctaccagaa gctatttcag tagtaataac
    51721 tggacttggt tgagggtcta tattattgaa atacttagat gatggtagcc ctacactctt
    51781 ctttaaaggt tttgtacagt ctttatattt aaaaggtccc atttcaagat tttccaagtg
    51841 ttctctccaa acccaacctc ttcgttttgg tttatattta tccaaatctt ttaatatata
    51901 gtctatatcc aattttttat tttcctttaa catgattcta ccccttctct aaataaacat
    51961 gtaacttcat cccaatattc acccttaact aataacaaac ctgcttctct aaccgttata
    52021 tttttagatt tagccaattt ataaactaca tgccctgctc ctttacctat aagacctctt
    52081 tccataaccc cttctaaaat aggttttact tcaagagaag aaaatcccat tctcaacaag
    52141 acagaccttt ctattgatgg tgttgtattc tttttaccaa gttctatcat tggttctact
    52201 atctgggtag ctaaactcca aaacctttct tttaactcat catcactcag ttcttgaaga
    52261 tgttttcttc taacttcaaa atcatcctct ctttttttca tgaacattcc tccattcgta
    52321 ttgatttaat cgaattttct gaatttttat tttatattaa attaaaaaac aataattact
    52381 ataaattatt taatacatca acaacaaaat ctcttgaact ttttacttct tccattaaaa
    52441 attctatatc tcgattactt agcttatcaa tattatcaat gtgattatta gatatacaat
    52501 tttttataaa tgactttctt attttattca tatcaatatc aacacatttc aataattctg
    52561 gtctttctgg taatattaag tttactcctg caatttcttc tcttggattt ccaaatttta
    52621 tatctattcc attttctctt gcaaaagtaa gctgtggaca tatatgttta cctgcgcctg
    52681 tatattctgt ttcttgtacc actataactt tatcctcatc taactcttga gctaaagaga
    52741 atgctgctgc taaagatgta tttcctgctg gacctctttc tataccttct agctgtgcca
    52801 ataattcagt tgtataaaaa acttctcctt gttttactaa tacatatcta tccatatatc
    52861 taagtggtct agctgcagac ctaggtacat ctgacctatc aggccatgta cagtatggta
    52921 tcccaaaacc tgtatgtcct gttgtaaatg attttttatt aaattgatta tcactagcca
    52981 tgtgtaatcc ttttaaatct acacttgctc ctactacttt agtattaata gaaccagctt
    53041 ttatcaatcc tcttgcagtt ccagtaagat taccaccacc tgcatttgta caaacaacta
    53101 tatcagggtc ttttttatac ttttctctaa attggatagc taactcataa cctaaagttt
    53161 ctacacctgc aattccaaat ggtgaatata atgatgcgtt aaaatagcct gtttcttcta
    53221 ataaaactaa gaacgtataa aataactctg gtccaacagt taactgaaca acttctgctc
    53281 catatgcctc acattttcgt gctttttcta taatttcagg ttgacctaca ccattagaat
    53341 cataacattc ttgaactatt atacatttta atccctgcat tgccgcttgt gatgctacag
    53401 ctgcaccata gttaccacta gtagctgcta ttacaccttt atatcccatt tgtttggcat
    53461 gatatactgc agtagctgct cttcttgctt taaaactacc agatgcattc attgcttcat
    53521 cttttacaaa tattctagct cctttacctt ctttagcaca ttttctagct aatttagtca
    53581 aatttttaag ctctattaaa ggagtatttc caacagcata ctgtgattga attgcttcaa
    53641 tatcttgtaa tgtatatcct gtttcactca tcattttctc ataatcaaac cctattccct
    53701 cttgttcaaa ataagaataa tccaatccga ttgcattttt cattatttcg ttatttcttc
    53761 ccataacagc ttgataactc atatctttca ttgatgttga attattcatt atatacacct
    53821 ccaaataata tttctttagc ttggattcct atttggagta actctggtat acatttacca
    53881 taatcatgag tataatatgg atttatttta agtaaattac cttttacaag tctgcctgta
    53941 attgttttaa cttctactaa gtcgcctata gaagcatcac tttgtataaa acctttaacc
    54001 cacatttcta aggatacttt cttagtatcc tctggaactt gtggagctct ttcttctgga
    54061 gtcaatacta tattgtgtat aataacccaa tcattaattt tagcatccat tttttcatcc
    54121 ccttattaat tgactattgc cttactaatt aatttactgt tattttaaat taaacattat
    54181 tttaccaatt agctaactgt tatcttacta attagctagt tgctatctta ctaattagtt
    54241 agctgttatc ttactaatta gcctttgatt cttcttccaa ttcaacttct atctgatttc
    54301 ttatatctcc cattattgct ctaggtactg gtatatccaa catagtttta agacctggtc
    54361 tagcatttat tattaatgga atagagttta ctatcattgc ataagtacct actcctccag
    54421 gtatttcagg atttatttgt aaatcaatgt ttggtatacc ttttattgat acatagtctc
    54481 ctgttttaat accttctgct tctggtatta tctgttgtgg atgctccatt tcaattaaaa
    54541 cttctccatt tacatatcca tatccacatt gtctacaacc agcaacattt ccagccaatg
    54601 cctctccgta cttagatttt ctatctactg tagtcattat tggctctttt gtttgttcta
    54661 tcttatctaa tttccaacct aatccatcag ctatcatatt tattgattct acaaaaccta
    54721 catgacctgc tatagtctta tcttctacac cttttataaa ttcttccctt gttactccaa
    54781 caccttgttc ctccataaca gactttccaa atggagataa atcatttact ctcttagctt
    54841 ttatactagt aacttcttca caagtgcctg aaagcgccaa tactagtaaa tctaatacaa
    54901 atcctggatt tattcctgtt ccaagtatac ttactccatt ttcctttgca acttcatcca
    54961 tttttttagc taaatctgca tcatcagctt gtggatatgc catttgttct gctgttgata
    55021 taacatttat ttttctattt aataagaaaa ttattttatc aaaagccttt ttagtaaatg
    55081 aatcagttgc taacaaaaca acatctacac ttttttctct aaaaacttct tcataatttt
    55141 tattgataat aacttctggt ctttctcctc tttcaattcc taaaacatta tacatacttt
    55201 ttcctgaagt acttctacta caaacagata ctatttctat accttctttc tttagaatca
    55261 tattagccat acctattccc atagctccaa atccccatat tcctactctt acttttctca
    55321 tatatacacc ctcctaaaaa tttatatatt ctcaataatt agttaaagca taaatcgtgc
    55381 caactttaaa attaaagtaa atatttattg aaacgttgaa actataatta ttttattatg
    55441 tattttcata aataaaaaat aatatttctt atgagaaata aaaaaaagag caaaatattt
    55501 tgctctttta taataatttt gctcctctat tttgcttttt tattttactt tgattttgtt
    55561 tttttatctt gcttatttat aatattttga tattatattt ttttatttta tactgtaaat
    55621 tttgcctatt catacctaaa attgatgcag ctttagttat attgtaagat gccttttcta
    55681 atgattcata tatatacttt tcttctatct cactgataat cttactaagt gttttgtcct
    55741 ccaaatcgtt taaagaataa ttgttactct ctttattctc tttaagattg taatagtcgt
    55801 ttagattaat catacttgtt tctatttgta cctcatcttc catcatactc atagtacaat
    55861 aaattatgtt ttctagctct cttatgttac cttgccaatc gtaatttttt aaaattttaa
    55921 tagcttcatc tgaaattcct actacttttt tatttaactt tttattatac tttgaaataa
    55981 attttttgca caatagaggt atgtcctctt ttctatctct aagaggagga atttttatat
    56041 gaattatgtt aagcctataa tataaatctt ttcgcatttt atgctcgttt aaaattacct
    56101 ttggactttc atttgtcgtt gcaataattc ttacatctat aggtatatcc ttagttcctc
    56161 caacccttct tacataacct tcttgtaaaa cccttaatag tttagcctgt aacataatag
    56221 gcatggaatt tatctcatcc aataacaatg tccctttgtt agcttgttca aataagcctg
    56281 gcctatctat tgctcctgta aatcctccct tgctagttcc aaaaagaata ccttcgaata
    56341 gtgattccgg caaagctgca caattttgtg ctataaatgg atagtttttc ctattacttc
    56401 catagtgaat ggactggcta aataactctt tacccgttcc agtttcaccg tatataaata
    56461 ctgaagcatc agacttgcaa gctttcttag atttagctat tgcttcttta atcaaattac
    56521 tttcccctaa aatatcatca aattcataac cctttttttg tctaatacta actttattga
    56581 ctaaattttt tttataattt ctgtttagta aattttctat cgttgtcata ttctttgata
    56641 tctcaagtgc ccctacaaac tcgcctttaa tatcaattgg tattgtagta ttaattgctg
    56701 ttatctctct tccattttta tccagatatt tttgaatatt gtctttgata acttcccttt
    56761 tttccaaaac ttctaatagt gtactcctgt ttattttcat tttgttgact atatcaaaaa
    56821 atgaattggt taaaatatcg tgtctgttca ttttttccat tttttgtaca gaattattgt
    56881 agattatagt tttcccgtct ttattaacaa aatgtacccc ttcatcaata ttttccaata
    56941 tttcattcat tattttaaaa aatatctctt ggttcaacta tatcacccca gtacctaaat
    57001 atataaaaac ccaaaaaatt aataaaatgt tgctattgta tttatcttat cat
//
LOCUS       DAYATN010000009        55931 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.009, whole genome shotgun sequence.
ACCESSION   DAYATN010000009 DAYATN010000000
VERSION     DAYATN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55931
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.009"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            257..1021
                     /locus_tag="ACGI8A_000566"
     CDS             257..1021
                     /locus_tag="ACGI8A_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFH0178578.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(1302..2417)
                     /locus_tag="ACGI8A_000567"
     CDS             complement(1302..2417)
                     /locus_tag="ACGI8A_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFH0178579.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(2458..3984)
                     /gene="glpK"
                     /locus_tag="ACGI8A_000568"
     CDS             complement(2458..3984)
                     /gene="glpK"
                     /locus_tag="ACGI8A_000568"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFH0178580.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            4277..5374
                     /locus_tag="ACGI8A_000569"
     CDS             4277..5374
                     /locus_tag="ACGI8A_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFH0178581.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRY
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(5865..6377)
                     /locus_tag="ACGI8A_000570"
     CDS             complement(5865..6377)
                     /locus_tag="ACGI8A_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178582.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            6930..7811
                     /locus_tag="ACGI8A_000571"
     CDS             6930..7811
                     /locus_tag="ACGI8A_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HFH0178583.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKSVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(8124..8903)
                     /gene="hisJ"
                     /locus_tag="ACGI8A_000572"
     CDS             complement(8124..8903)
                     /gene="hisJ"
                     /locus_tag="ACGI8A_000572"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HFH0178584.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNKILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLDLVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(9449..9799)
                     /locus_tag="ACGI8A_000573"
     CDS             complement(9449..9799)
                     /locus_tag="ACGI8A_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HFH0178585.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(9815..10231)
                     /locus_tag="ACGI8A_000574"
     CDS             complement(9815..10231)
                     /locus_tag="ACGI8A_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HFH0178586.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(10250..10441)
                     /locus_tag="ACGI8A_000575"
     CDS             complement(10250..10441)
                     /locus_tag="ACGI8A_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HFH0178587.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(10592..11407)
                     /locus_tag="ACGI8A_000576"
     CDS             complement(10592..11407)
                     /locus_tag="ACGI8A_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFH0178588.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(11454..12392)
                     /locus_tag="ACGI8A_000577"
     CDS             complement(11454..12392)
                     /locus_tag="ACGI8A_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0178589.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(12389..13261)
                     /locus_tag="ACGI8A_000578"
     CDS             complement(12389..13261)
                     /locus_tag="ACGI8A_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0178590.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(13286..15214)
                     /gene="acsV"
                     /locus_tag="ACGI8A_000579"
     CDS             complement(13286..15214)
                     /gene="acsV"
                     /locus_tag="ACGI8A_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid activation/regeneration protein AcsV"
                     /protein_id="HFH0178591.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(15326..15703)
                     /gene="gcvH"
                     /locus_tag="ACGI8A_000580"
     CDS             complement(15326..15703)
                     /gene="gcvH"
                     /locus_tag="ACGI8A_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFH0178592.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(15790..17916)
                     /gene="acsB"
                     /locus_tag="ACGI8A_000581"
     CDS             complement(15790..17916)
                     /gene="acsB"
                     /locus_tag="ACGI8A_000581"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HFH0178593.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(17973..18779)
                     /gene="acsE"
                     /locus_tag="ACGI8A_000582"
     CDS             complement(17973..18779)
                     /gene="acsE"
                     /locus_tag="ACGI8A_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase/acetyl-CoA
                     synthase methytransferase subunit"
                     /protein_id="HFH0178594.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(18829..20196)
                     /gene="acsC"
                     /locus_tag="ACGI8A_000583"
     CDS             complement(18829..20196)
                     /gene="acsC"
                     /locus_tag="ACGI8A_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HFH0178595.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(20227..21171)
                     /gene="acsD"
                     /locus_tag="ACGI8A_000584"
     CDS             complement(20227..21171)
                     /gene="acsD"
                     /locus_tag="ACGI8A_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HFH0178596.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(21191..21961)
                     /locus_tag="ACGI8A_000585"
     CDS             complement(21191..21961)
                     /locus_tag="ACGI8A_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HFH0178597.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(22017..23402)
                     /gene="lpdA"
                     /locus_tag="ACGI8A_000586"
     CDS             complement(22017..23402)
                     /gene="lpdA"
                     /locus_tag="ACGI8A_000586"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFH0178598.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTIKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(23446..24327)
                     /locus_tag="ACGI8A_000587"
     CDS             complement(23446..24327)
                     /locus_tag="ACGI8A_000587"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFH0178599.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(24362..25036)
                     /locus_tag="ACGI8A_000588"
     CDS             complement(24362..25036)
                     /locus_tag="ACGI8A_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HFH0178600.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(25073..25945)
                     /locus_tag="ACGI8A_000589"
     CDS             complement(25073..25945)
                     /locus_tag="ACGI8A_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HFH0178601.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(26037..26669)
                     /locus_tag="ACGI8A_000590"
     CDS             complement(26037..26669)
                     /locus_tag="ACGI8A_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HFH0178602.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(26774..28450)
                     /locus_tag="ACGI8A_000591"
     CDS             complement(26774..28450)
                     /locus_tag="ACGI8A_000591"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFH0178603.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(28699..29478)
                     /locus_tag="ACGI8A_000592"
     CDS             complement(28699..29478)
                     /locus_tag="ACGI8A_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0178604.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(29788..31707)
                     /gene="cooS"
                     /locus_tag="ACGI8A_000593"
     CDS             complement(29788..31707)
                     /gene="cooS"
                     /locus_tag="ACGI8A_000593"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HFH0178605.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(32314..33225)
                     /locus_tag="ACGI8A_000594"
     CDS             complement(32314..33225)
                     /locus_tag="ACGI8A_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HFH0178606.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(33341..33919)
                     /locus_tag="ACGI8A_000595"
     CDS             complement(33341..33919)
                     /locus_tag="ACGI8A_000595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFH0178607.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            34145..35068
                     /locus_tag="ACGI8A_000596"
     CDS             34145..35068
                     /locus_tag="ACGI8A_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0178608.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(35240..36382)
                     /locus_tag="ACGI8A_000597"
     CDS             complement(35240..36382)
                     /locus_tag="ACGI8A_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFH0178609.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELRINK"
     gene            complement(36400..38154)
                     /locus_tag="ACGI8A_000598"
     CDS             complement(36400..38154)
                     /locus_tag="ACGI8A_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HFH0178610.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            38290..38496
                     /locus_tag="ACGI8A_000599"
     CDS             38290..38496
                     /locus_tag="ACGI8A_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178611.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(38501..39424)
                     /locus_tag="ACGI8A_000600"
     CDS             complement(38501..39424)
                     /locus_tag="ACGI8A_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178612.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(39678..41378)
                     /gene="argS"
                     /locus_tag="ACGI8A_000601"
     CDS             complement(39678..41378)
                     /gene="argS"
                     /locus_tag="ACGI8A_000601"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFH0178613.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(41840..42253)
                     /locus_tag="ACGI8A_000602"
     CDS             complement(41840..42253)
                     /locus_tag="ACGI8A_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFH0178614.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(42321..44699)
                     /locus_tag="ACGI8A_000603"
     CDS             complement(42321..44699)
                     /locus_tag="ACGI8A_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFH0178615.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            45018..46469
                     /locus_tag="ACGI8A_000604"
     CDS             45018..46469
                     /locus_tag="ACGI8A_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HFH0178616.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(46673..49186)
                     /locus_tag="ACGI8A_000605"
     CDS             complement(46673..49186)
                     /locus_tag="ACGI8A_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0178617.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(49409..50230)
                     /locus_tag="ACGI8A_000606"
     CDS             complement(49409..50230)
                     /locus_tag="ACGI8A_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0178618.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(50748..51224)
                     /locus_tag="ACGI8A_000607"
     CDS             complement(50748..51224)
                     /locus_tag="ACGI8A_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFH0178619.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(51258..51836)
                     /locus_tag="ACGI8A_000608"
     CDS             complement(51258..51836)
                     /locus_tag="ACGI8A_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFH0178620.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(51940..54522)
                     /locus_tag="ACGI8A_000609"
     CDS             complement(51940..54522)
                     /locus_tag="ACGI8A_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFH0178621.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSSISMDLYTSSSLGIPKELMGSNTNNIYTLSNTSKIDTLNKNHSNTA
                     VTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNIDCYLNA
                     FNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVTEYCPMG
                     VVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLDNLYELE
                     EIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKRATDLYKKLDEIGTVAGHYY
                     KGVE"
     gene            complement(55092..55775)
                     /locus_tag="ACGI8A_000610"
     CDS             complement(55092..55775)
                     /locus_tag="ACGI8A_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFH0178622.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT        17410 a        10492 c         6265 g        21764 t
ORIGIN      
        1 atctctatac taataaatta tataataaaa tcatatttta gtcaattgtt ttataaaatt
       61 tgagttattt tgaattatta aacatctatt tttacttttt tgcttaaatg taagttttaa
      121 atatggtaaa attatcttgc ataacactta taatattatt tgataaatat acatacatta
      181 acaaatatac ctaaactctg acttaattgt aagttatgcc aaaactaaaa taatactagt
      241 ccaaggagga tattttatgt taccacagga aagatatgag aagattttaa gtattctcaa
      301 atcagataat ataattaaaa taaatgacat agttcaaatg tttgatatat caatagaaac
      361 tgcacgaaga gatttagccc atatggagag tatcggtctt ttaaaaagag tttatggtgg
      421 tgctatacca gttatagcaa gtagcgtaga acttaactat gttaaaagaa aaaaaataaa
      481 ctcaaacgat aaaaaacaga ttgctattaa atgtagtgag tttataaata caaatgatac
      541 tatttttatg gatactggaa ctactgtact tgaagtttca aaaaatttga aagataaaaa
      601 gaatctaact gtaataacca actcaatttt agttgcaaac gaacttatga atactaatat
      661 aaaagtatat gtgcttgggg gctatcttag agaaactgaa ggttcttttt ctggtccgct
      721 tacactgtca gctattgaac aatttaatgt tgataaagct attttaggcg ctggaggtat
      781 cactcttgaa aatggaatct ctgactatca cttagaagaa gcacttgtta gaaaaaaaat
      841 gattgaaagg tctagaaaat ctatagtagt tgtcgaacct cctaagtttg gtataaattc
      901 atttgcatca gtagctccta tagaacatat agatactatt attacctctg ctagtataga
      961 ccataatata ataatagagt tttctcaaaa aggaatacct ttagtcatag ctgataatta
     1021 gcttatatga ttgattttta gttccttgat gtaataaatt aaaattaagg agccaaataa
     1081 ttatttattt agctccattt atgtctaaac tatactttat attttaatta tgtaactact
     1141 ttcagttagc ttatcatttt tagttatttg attgccattt tatttaattt ataagtttta
     1201 gttattcgat tattttttaa ttgtctgtct ttttaattta tttttagtta tttaaactcc
     1261 tactttaaaa taatttaaat ttgacttaag tattgtttat tctacactgc atctttcttc
     1321 attttaatac tttgttcttc ttccaaggtt aaatcttttg tcataagtat agcaagtgca
     1381 attgccaata ctcctccaac caattttcca attacaacag gtgtaatcat ctctggaact
     1441 actcctgctg taaatcctaa atggtccccc aaagctgctg tagcacaaac cagccaagca
     1501 gtatttacta ctttcccctt tggactcata tctttcatca ttttatatac tggaatacta
     1561 ttggctaaag taaaaactaa tcctgctgca ctagtggcat ccatacctaa ttttccaccc
     1621 actattgtaa gtggtttatt aagaacttta actaataaat gtaatattgg aaaagtacct
     1681 aataacacga ttgcaatact tccaattact tgaatacctt ctcttatagg tgtcatgcct
     1741 tttataagta ctatacctgt tatttcttga aatgctgctg ctcccaaacc tatcgttatc
     1801 aatatagata taaattttcc aaaaattaat gacccattta tcataagagt agaattaaat
     1861 tttaaaccta ctgccaatat tactgccaat attatcactg gtatcatatt tataattacc
     1921 attgataagt taaatcctgc tattaatcct cctataatac ctccaaaggg tattgttatc
     1981 aagcctatca gtaacccttt tgcaaagaat ggtctgtctt catattctat aagacctagt
     2041 cctactggaa ttgaaaatac taatgtacat ccaagcatgg atgacacaac caatcctgca
     2101 agcattcctg cctctttatt gtgtgcaagt tccatagcca atggataccc tcccatatca
     2161 ttcgctaaaa tagttgcaaa catagatggg tcagctccac agagattgaa gattggtatt
     2221 attataggac ctagtacatc tgaaattact ggagctaaac aaactatacc aaccattcct
     2281 aaagccaatg gacccattga attaaaccct tcttcaaact gctcgccaag tccaaattta
     2341 tttccaatta ttctatctaa cgctcctaaa agcactccca cagccattat gtataatata
     2401 attttatcaa agggcatttt taattcctcc tatacatgta aaatactcta ttgtaactta
     2461 attagcacac acctttttgg tatcgttttc ccaattcata gctcttttta ctgcttggtt
     2521 ccatccaagt aatatttctt ctcttttttg agtagtcata tttggaatat attttttgct
     2581 tattgtaaat tcacttctaa tttcatcaat actgttccag acaccacaag aaagacctgc
     2641 catatatcct actccaagta ctgtcgtctc aggattttct gcaatttcaa ttggtatacc
     2701 taatatgtcg gattgaaatt gcattgtaaa gttattttta gtaattccac catctactct
     2761 caacattttt atttttttcc ctgaggctaa gttcatagaa tcaacaacat ctttgacttg
     2821 aagtgcaaca gactcaagag tcgctcttac tatatgctct tttttagtac ttcctgttat
     2881 cccaaccatt gtacctctag catattggtc ccaataaggt gctgctatac ctgcaaatgc
     2941 tggtacaaaa tatacacctc cagtacttga tatggaattt gccatgtcat cagtttctga
     3001 ataatttttt ataactccaa tgccatctct aagccattgt attgcagcac ctgctatata
     3061 cactccacca tcataagcat atgtaacttt atctcctatt ttccaagcaa cagttgtcaa
     3121 aataccatta cctgctatat ttagttcctc acctatattc atatatagga aacatcctgt
     3181 accataagta tttttaacca ttcctggttc aaaacatcca ttgccaaata aagctgctgg
     3241 ttggtctacc attgaagcag taatttttat agaaacccca caaaatgcct ccttatctgt
     3301 agttccaaaa tcaccactcg taggcattat atctgctaaa atacttctag gtactcctat
     3361 aatgtcaagc aactctctat cccaatccat atctcttata ttaaaaagca ttgttcttgc
     3421 tgctgtagat acatcagtta aaaaagattt tcctcctgtc attttccata ttaaccaact
     3481 atctaatgtt cctgcaagta cttctccatt tttaattttt tcttttacac cttttacatt
     3541 atctattacc catttaattt ttgtacctga gaaatatggg tctataatta acccagtctt
     3601 atctattatc ttattttcta aacctggtat attctttaaa ttttccacat aatcataagt
     3661 tctcctgcac tgccacacaa tagcattgta aagtggttta cctgtatttt tatcccataa
     3721 cattactgtt tcaccttgat tatctatacc aattcctttt atatcacttt cattgattcc
     3781 attttcaaaa gcctgttgta atacattatt actcacagtt tttgtattat tccaaatttc
     3841 tatagggtca tgttcaacat atccagattg tggatatatt tgagtatgtt ccatataatc
     3901 gctaaaaaca aactctgatt tttcgtttat caatactgct cttgttcctg ttgttccttg
     3961 gtcaattgct aatacatatt tcactttaaa tgtcctcctt tattttcaga attaataaat
     4021 tcttttttat tttttgaata tataatctat tcaagttatt tatattttat atcatcatgt
     4081 ggatttatgc aatattcatt acaatatttt ttgttatttt taattatagt gattgaaaaa
     4141 gtatttttat tgaaatattg ttgcaatatt tattatttta ttctataatc tagttaaaat
     4201 aacacataat attttaatat ttacacataa tcagtcattc aaaaatttaa atttaacttt
     4261 ttaaggaggc tttacaatga aaaaaacata tctattcagt cctggaccag taatggttac
     4321 agataaagtc agaaattcac ttcttcacta tgatatatgc catagaggaa atgagtttat
     4381 ggatttattc aaagatacac aacaaaaaat caataaacta tataatgcaa cttctaatta
     4441 ctactcatta attgtatctg gctctggaac atctgtaaat gaatgtgtac tatcttctat
     4501 ctttgacaaa gaagatgcag ttctgctagt tagtaatggt gtttttggag aaaggctaga
     4561 agaaatatta tctacttata atgtaaaaac atataaacct aattttgaat gggcagaata
     4621 cccggaccta gacattttag aaaaatacat aattgaaaac tctgatataa aagtaattgc
     4681 tatggtgttc catgaaacta gttcaggaat gataaatcca gtccctgaac taggaaaact
     4741 tgctaagaag tataacaaaa tattttttgt agatgcaata agtgcctcag caggtgaata
     4801 tattgatgtg gaagagttta atatagatat aataacaggt gttggtggca aagctcttgg
     4861 agcatttcca ggttctgcat acctttgtgc taaagagagt attttacaaa atattaaaga
     4921 aaatcaatgt aagaatgtgt atcttaatct ttataaacac tataaagttg ctaaatcatc
     4981 ttctcaaaca ccaaatacac ctaatgttac tttaatattt gcccttaatg aagctttaac
     5041 tgagtttttg gaagatgaca gtaaaataga aagatacaaa gaatgttctg ctattttaag
     5101 gtatggtatg gaagaattgg gtctaacatt tttacttcca gataagtata tgtctaatac
     5161 agtaacatct gtatttctac ctaaagaaat caatgtaaat gagtttatat tagggctaga
     5221 aagggaaagt ggctatgttg tctatccagg aaaaggcaaa ttcctagatg ctaacatatt
     5281 ccaagtagca aatatgggag aaatatatcc agaagattgt tataaattct tagatatctt
     5341 aaaatcaaaa ttggagataa aaaaagttat ataacagttt gtttgtaaaa tcctacaata
     5401 tatcagtata aattaaatag actgattttt ttaaattttc ctataataaa ttatatttta
     5461 tatttttgta taagggtgaa ctataatatg taaagttcac ccctatttca aatctttttc
     5521 cttcaatata atctttttct tttaatttat tttatatttt tgacgcaatc gttttttatt
     5581 caatcatttt atattcttga tacaatcatt cttacactta attatttttt gtttttgaca
     5641 caatcgtttt ttgttgaatc attttatatt cttgatacaa tcattcttac acttaattat
     5701 tttttgtttt tgacacaatc gttttttatt cagtcattta atattttcaa cacaatcatt
     5761 cttacattct attattgttt gtttttgata caactgtttt tattcagtca tttaatattc
     5821 tcaacacaat taccttttca ttcaatcaaa gtaatcttta agcattattt ttcaagctca
     5881 tcaaagtaat tatatacgat aacctcacct gtctctttat ctgtagtctt tataatgccc
     5941 ttagctccat ttacctcttc ttttgtaaat tcatcatcta acacaacttt gaatttatct
     6001 tcctttccat aaccttgttg aaattcatct ctcaaaattg catttttttc tgcttttggc
     6061 ttcaactcaa attcaattcc agatttcatt tcatcaaaat aatttaatac tacaacctca
     6121 ttattcattt tattagttgt ctttataatc cctttagctc catttacttc ttcttttgta
     6181 aattcatcat ccaatgttat attaaatttg tcttcttttc catattcctc ttgaaaatcc
     6241 ctcttcattt cattgtattt agtttcaaac tctttactct tgtttaattc catagctgat
     6301 acatttgtca atgatgctcc aattgccatt actgttaatg ccattactat accactttta
     6361 aattttttta ctttcatatc ttacctccta aattaaatgt tctcaatgaa attatataaa
     6421 gaaaatccta agttttacag tttcattctt gtaactaaaa ttactattat gtaaccaaac
     6481 tttttgcttc aactattcct taaaagttgc ttttttgagg aatagttgaa gttaaataat
     6541 tagattttat ctatatttat agatttaaaa tcaaatttat ccatataaat aaatacttat
     6601 ttttgcaaaa taaaaatttt taattactcc aatccttctt atttacttaa aatcctatga
     6661 taagaaattt aaaagtcttt accttcttta acaataattt atttactttt aacatacaaa
     6721 atatagagag tatatcaaaa ctattttact gaatattcta aaaataagta ccccctatta
     6781 gtatatatat ttacatatta aaaaaaagtt ttaaataaag ttatattttt tacaacagtt
     6841 ttgtaattaa aaacatattg aaggtaaatt taaaggtact atatactata aatatgtcga
     6901 cacaataaat ttaaaaagga gaactaaata tgaaaaaaag attaataata atgatattgt
     6961 ctgttgtctt agtactatca agtatactta cgatatttgc atattcaaat attaaatata
     7021 ataataacaa attgatatat tctaatatga tagataaaaa aactcaaaat agtgtaaagg
     7081 aaatcctaaa agaaaataaa attaatgaaa aagacataga tacatttatt aaatcagtca
     7141 acaattataa taaattgcaa gttaaaatac ttcaaaataa tataaatata tctaaatctg
     7201 gatattcttc aatcaatgca aaacaagttc catataacct agaaaaattg caagataatt
     7261 gggtgaaaaa attccctgat tatatggatg taaattgtag aataactgct tttagacttt
     7321 ttaaagattt tataaattca aataagaaat ttactggaga ctctattgat ttgaatgtcg
     7381 acttggacac tataatgaat aataaagatg ctaaatttag caccaaagat gtagaaaaat
     7441 ttatcaactt cttttctgca ataccagcta aagatacaga tgatacaatc aaaattgcag
     7501 aacaaataaa aaatgaatgg aaaaaaagaa aaatatcttt taaaaataat aaaaatatat
     7561 ctattataaa tggatttctt cgttatccag aaactaaaaa tgtatttata ggtcatacag
     7621 gcatatgtat taaaactaaa aatggcattc tatttttaga aaaatacggt gttacatcac
     7681 cttatcaagt tactaaattt aaaaataaag aagatgtaaa aaattatatg tttaatagat
     7741 tgaagatgtc agaaggtgaa attgaattac ctgaccctat aataatggaa aacgataaac
     7801 ttatgaaata ataactttaa acgcaaaata gtatttataa ttactaagtc aatataatag
     7861 agaaggtcta tagatttgtg taaatacaag cttatctaca atcttatata agaatgttta
     7921 tataaataag atagattaat tctaagtcct attattctat tgaattttat tattatttta
     7981 tttagattct attgttattt tacttagaac ttattattat tttgtttaag ttctattact
     8041 attttcaatc atataaattt acaaaagact gtacataaat ttattttata caaacttatc
     8101 tacagtcttt tgtgtgaata tatctaaata atatcgtatt tatttatttc ctctgcacag
     8161 tgagaatcag aacctaaaac taatggaata tcgtattttt ttaccaagtc taataagtat
     8221 ccatctatat aaggctcttt gcagtactct tttctatttc cagaaacatt ataatcaagt
     8281 tcataacctt tttctttaat taactttatt aactcttcaa gaattttatt tcctttataa
     8341 tcgtatggaa atacctgatt aaactttcta actaaattta aatgacctat tctttttggt
     8401 ttatgtatac ctaaatctga atttacagct tttatcaatg tttcataata cttattataa
     8461 acagcttcaa tacttcctaa tttgtcgatt aaattttgaa actcctctac actgtaatct
     8521 acacaataat aatcatcatc aatcttaatt atatgtactg ataaaagaga atcctctaat
     8581 tcctttccat atttatttaa attctccttt atcccttctt cgtatccttc tataaaatcc
     8641 acttccgacc caacatttat cttgatttta ccctcatact tttctttcaa cttcttgaca
     8701 tcattaaaat actttggtaa atcttcaata ctcatacaac tatcattttc aggagcaggg
     8761 tctctaaatc catttgcata cggaaaatgt tctgtaaatg tcatttctgt tattccaaca
     8821 tccattgctc tttgtacata tttttcaaaa tcatccttac ttccatgagg acaataaggt
     8881 gaatgtatat gaccatcttt cataaataat tcactccttt tttaaagaaa aaatctaata
     8941 taaatgcata aatcttttaa cttcaaaata atatttttaa tcatgctaat ttttattata
     9001 tctaatatta gagtaatttt aaactgcctt ttaagtatct tatcattagt actattttca
     9061 acttaacatt aatattattt tttaacttaa cattaatact atctttaaat agtaatactc
     9121 aatatataat tacttaacat aaaataactc aaattgcttg atgaggcttt tgcagtattt
     9181 aaagaatata taattattta acattaaata atccaatctt gtaataaaat ttactcatta
     9241 aagtatatta cagaaaatac ctcaaaataa actttttagt tcatgatata ctcttttttg
     9301 catgaaatta gatatatttt tatcatttca acaatgaaag ggaggcttat atccatcttt
     9361 agacaacccc ttagataaaa tttttaataa ttaaacatca caaaatataa aaatcgctta
     9421 tatcaaacgt ataagcgatt aattatattt acatatgttt tttagcctct aacaacattt
     9481 tattagcttc ttccatatac tctatagcct tttcaatact ctttgctgtt tcttcttttc
     9541 caatagctct agccttttca acccactctc taaatccttc ttcatgagtt tcattgtgat
     9601 ttatccaatg tactaaaagt atttttaaag ttttttcatc cttagactct gatgattcct
     9661 catgactatg ttcgtggtcg tggtcgtgag catggtcatg tggatgttca tgacatatat
     9721 ctccatgagt atgttcgtgg cagtggtcat ggtcatgatg tccatgatga tatcttacaa
     9781 tctctttatc aaaaatcatt agttattccc cctgttattt tatattaaac tcttttttta
     9841 taaggtttac agaatctttt acaagtagtg ctaatggttt accttctaat ccaactaact
     9901 ccacattttc tggcataata ggtaaaagta tcttaagtgc ctcactgtct gcaatagctt
     9961 cacttatatt acaagtaact tctcccatca tagaattagg tattattaca gaaataggtg
    10021 caactattat atttgctttt ttagctgata ctactatagc attttcccct gtagctcctt
    10081 tattagcatg agctttcatc atagaactag ttgctataga attagtccct agtgcataaa
    10141 tttctacata tgtaggtaac tcttctctca aagaagaaac tatctgggct ccaatacctc
    10201 cacccattcc atctatcact gctattatca tatgaaattc ccctattaat taattttctt
    10261 gtattattat cttatggtcc atcaatctta tttctttaat tatcccatct attatctttt
    10321 gctctcctaa taaatccgtt aaataaatct ttccatcaca agggtctata ttaaccacat
    10381 tttccataac cttttctaac tcattattac ttttacaaat gaaagctgat gattcacaca
    10441 tataaatctc ctcctaatat gatttttatt aaaaatcaaa aaaagattcc cttaggtatt
    10501 tcaatacacc aaagaaacct tcatgatttc agtttttgta tattattata ccttaagtat
    10561 atcactttaa tttatgtaaa gtcaattaaa gttaacgctt tttattatac tcttcattct
    10621 tgtatttttc atatagttct ttaactaatt gcttctcata atcagtcaaa tcgccctcca
    10681 caaactcaac attaaattgt tcagctaatc cctttactat gtttttctgt agaatatcta
    10741 tgtctatttt tctgccaatt tcattttcta ttccacttgc ttttttagct gtaaatttca
    10801 tagctctttc tttcaattct ggtgtcttag tttttattaa tttaaacaat ttttctacat
    10861 caaaatccag tattattgaa ccatgttgta ataaaacacc atcttttcta ctttgagcac
    10921 ttccaattac cttcttatta ttaatagtaa cttcatatga tgcatgtgca ttaaaacatg
    10981 cagctgataa attttcccta cttattctct cgcctctatt taaattatct gtttctatac
    11041 cacttagatt caacccttta actagacctt cacttatata tctataagat aggttgatgc
    11101 tcttatcgat taaaggatta tcttctccta taattatact ataggttagc tcattatcat
    11161 gtaatacagc tcttccacct gtaattcttc tagtatagtc tatattcata catctacact
    11221 tatctaaatc tatctcatct tccaatttct gaaaatatcc tatactcaaa caagctggtt
    11281 cccatgtata aaatctaaga gttggcttat tatgtccttt cttatatgca gtaaatatag
    11341 cttcgtcaat tgccatattc attgctcctt catatgattt attatgtata actctccact
    11401 gattcatgat tattacgcct ccatttttag tataaatata tcctagaaat cctctataga
    11461 tttatttctc ttattgctaa atctatctca ctttgattta aaggtcttga atagttgtat
    11521 atagtacttc ctggtctttc attatagtat ggtctattac aatccttaca acctcttatt
    11581 tcaaaaggat agcctttgtt tatgtctttt aaaatatcat tgtcaagctt tatactattt
    11641 aagtatccat ttttaaactt aaaatactct tttggataac ctttatttat catataagac
    11701 attaactgta cccttctata actttctatg cttggttgat ttatcttttc cattttagtt
    11761 cctctaactg gagtaaatgc aaataaacta atcgttacat cattttcctt taaataagta
    11821 tataaattgt atatatcttc atgactttca cccataccaa ctattatatg tgtacttatt
    11881 ttattgggat atgattttga catttcagtt ataaatttta acttttcatc atagttattt
    11941 ccttttattt tttcatatag ttctttattt gctgcatcta ttgctattcc tattttatca
    12001 actcctgctg aaaaaaactt ctttatctgt tcatcatttt ctaattttgc agatagtgaa
    12061 attggcatgt ctatcttacc acttatatag tttataaagt ctaaaacaga gttatgtgct
    12121 tcttcacttg ccattgattg aatacatatt ctctttatat tttttccttt ataagctttt
    12181 aaagcatcta atatttcttc ttttgaaaac tctggccaaa ttactcttga taatttatct
    12241 tttctagtac tactttctat tgattgagaa caaaaactgc atttatttat gcatttttct
    12301 ccaatcatta tatatgctgt ggtaggaggt atatcgcttt ttttgtttaa tatcccaagt
    12361 tctattgctg tcccaactga tagtctaatc ataaaacgca acactcctta cactcattta
    12421 ttgtcatatt catttctata gccttattct ttgcagacct tgatggtaat actattctat
    12481 tgactccaca cattatagat aattggtcta gctcttttct atacactcct cttggtctca
    12541 tacaacctag attaatttca gtatctggta aattaattct agcttcacat aaaatatctg
    12601 ctaccccttc taactcagga ggttctacat tttcatactc agtccctttt gttggaataa
    12661 ggactataaa tgtaagctta ttaggaggat ttttttgtag gtattctata atttcatatt
    12721 caccctttat ttgtcctcct tttaaaccaa tacatatatg tggtgctact tctgtatgtt
    12781 cttgtattgt attatagact tcaatataat cttctttact ctttttcatc ttataaactt
    12841 ctcttattgt ctcatcatca aacactaaat caaaagaaac tatatctaaa tacttacaca
    12901 actctacaat gctatcttta tccataagac ctaaatgagc attcaatcta tatccttgct
    12961 cttttaagtt tttaatggta ttaatatgag tatttatagg gacatctccc tcataactac
    13021 aaccgccact caatagaaag ctagttatat tccttgattg tactttttcc tcatattctt
    13081 ctattggcac catattgttt aagtagtgac cgttgcaatg ggcacatttc aaactacatt
    13141 gattgcttgt agtactaaga gctagtgtct ggttaggata agcaaactct attttatttc
    13201 caaaattctt taatttaata tcaaatgcta ttttcatttt ttcagataga ttcttattca
    13261 tagtttattc cttcttaagt acattttact cttctagtaa tttctttgct gttgggaact
    13321 gctctatatt cgtatgtggt aagaaacatg cagaaataaa ttcatccata taacttggat
    13381 atacactaag ttcaacataa gttatttgat tacctatttc ttcaagttta ttttttgcat
    13441 ctttacttat aagcgctaag taagaaccaa caagtgaact attccctata taagtaaatt
    13501 tttctctatc aatatcagga agtaacccta taattattga gttttctatg tctaggttgt
    13561 ttcctatacc acctgctata taaaccctat ctaatatact aaaatccata cctaaactat
    13621 ctacaagagt gtatgcgcct gaatatatag ctcctttagc tctgatgaaa ttatctatat
    13681 caacttcatt gactactata tcattttcta aatcaaattc ttctttaaat gctaatacat
    13741 actctcctat ctcatgctca ttaaatctaa ctctcttatt atctaaatcc ctgtatattt
    13801 tacctcttct atctattaca cctttagtaa tcatttgaca gattaaatca attataccag
    13861 aaccacatat accaactggt gcacattcat caataatctt taatgttggc tctaaagtat
    13921 ctttatcaat aattactttc tctattgctc ctgctgatgc tctcattcca cagcttatcc
    13981 ctccaccttc aaaagcaggt cctgctgaac atgcacagct catcatataa tctttatttc
    14041 caaatacaat ctctccatta gttcctaaat ctataaataa aacattttct tcacttgacc
    14101 atatccctgc tgataataca cctgctgtta tatctcctcc tacataactt gcaacagatg
    14161 gagctaagta cacatatgca ctgtcattta caaaaagacc aacattttca cccataagtt
    14221 ttggagattt taaaaatggt ggtatgtaag gttcttgtct taagaagtct gtatacacac
    14281 ccaagaataa acttgtcata gttgtatttc cggcagccac taatgtaact acatcttcct
    14341 tatctattcc atttctttca tatattgttt ttaatagtgg atttatagtt tcctcaacta
    14401 tagctttatg aagtgtctct aatccatttt tcttagttga atatataatt ctatttataa
    14461 catctgctcc atattttatc tgtgcatttc cagaagatgc tttatctact acctcttttg
    14521 agtataggtc tactaaacaa acaactactg aagttgtacc aatatctatt gcaactccgt
    14581 ataaggaatt ttctttattt ccttgttcta tatttatgat tgtaagtttc ttttgttttt
    14641 gaacatatgt tattgtaacc ttaaaatcac tctttctaaa tacagttggc atttttctaa
    14701 gtatatccaa tctaaaatct atttcattat atcctaggtt atttctaaca tatctttcca
    14761 atctatctac atcacttata ttatcatcta aatttggttc ttccatttct atgtatttct
    14821 tttttatatt tgttttaaat tgaaggttat gttcttcaat tattttttta gctctttcaa
    14881 aaatttccct atcctctttt ttattactac cttctatctt cataccatgc atagaagatg
    14941 atactttaga aggaacttca atttctatat ctgatattac ctttgtacaa caagctagta
    15001 tataaccttg ctcccactca tcatctgtta tatgtcttgt tttttctgta tcaacttttc
    15061 cattcaataa ttttacttta cacttcccac aagacacgtt tccattacaa ggagcatcta
    15121 tgaatatatc tgcatttctt gcaacctcta gtaatatatc cccctcatta caataaacct
    15181 ctttattgtt tggtgtaaaa cttactttta tcatgttatc cctcccaatt tgttctatat
    15241 atgataaaag gaaagtatag ctaaatttgc gtgcactcaa attttactat actttcccca
    15301 attataataa tatcttaatc tttaattatt ctaatccagc ctcgtattct ttatcactta
    15361 ataacgtatc aagttcagcc acatcagcta gttttacttt taatatccag ttttcatatg
    15421 ggtcttcatt tatacattct ggctcatctt ctaatttttc attaacttcc aatacctcac
    15481 cattaacagg agatattaaa tcagaagcta ctttagaaga ctcaactact ccaaaatcta
    15541 ctcccttagt tacagtatct tctacttctg gtgtttcaac aaacaatatt tctcctaatt
    15601 gatcttgagc atagtctgtt atacctatat atacaacatc tccatcaatt acttttaccc
    15661 actcatgatc tttagagtat tttaattctg gtaataattt catttcgata gacccccaat
    15721 attatattat tgattaaatt acttaaatca tattaatatt atatcatatt attttaaatt
    15781 ttccatcaat tacattacac tttccatagc taatgctgga tgaccttttt cttctaagaa
    15841 agctaatact ccttcagaat cgtcagctat agtttcatca caaaccatat cacagaagtt
    15901 ttctattcca gtcatttctt taactgtagc atttaattta tcagctacat aatcttttaa
    15961 ttcttttggc atccaaacta ttctttctgg tccaccttct gcataagcga attttttaga
    16021 agatataaaa tgtcttccgt gtcccataaa tcctggagtt tgaactcctc cacctgtcat
    16081 agaagcaagt tctccgaaag tcataccaac tggagtaaca cttgcaaatt ctctgttaac
    16141 tacaacaaat ccatttgctt ctggcattat accacagata cattcgaagc atccacaaga
    16201 agtcattggg tcttctagta tagagtataa tgtaacagat tcaactgcac cttgagatat
    16261 ttggtttaca gtttcattta ctgaattcca tacacctgtt ctatcatcta agcactcgcc
    16321 tttttctatt ggttgacaag gacctgttgg gtctaattct ttagtagcct tagcatctaa
    16381 ccagctaact gctccacaaa gaccaagtct ttcaggagta acaacacaaa cgtgagctgg
    16441 tgcaaatgat tgacataagt tacaagaata gaaagtatca acactttcat caactaatga
    16501 agctaatctt tcatctctag cattgtactt agctattgct tctcctttta attcagaaac
    16561 tttttcagca tctgtaatta tagttatttc acacttatca actactgatt caaattcatc
    16621 taacatctta gcatataaaa cttctcctat atgctcaagt ctaaatcctt tatcaaatgc
    16681 atctttagag attcttaccc aagccatatc tctttgtcca acgtgcatta caccttctat
    16741 atagtttaag aagtagtgga aacgtctttc aagaactggc tcgaaatctt cttgcatatt
    16801 tttaccagct attttaacta ttacagcaag tggtaatctt aatacaccat ctgcatcaac
    16861 ttcatctata tttggcccta ttatttctat cttatggtct tcaacttctg ttgcttcttt
    16921 cttaacaaca agctcccaag cttcagttct gtttccacca aactcagcaa acatatcgct
    16981 ctttctaatt ctctcacctt caaatgctgc tgcaaatgaa actgggatag gtatttcagt
    17041 aacttttatt tttattcctc tagcttctaa tgaagtagct actatcttat cgtaatcttt
    17101 ttgagttaat aggttcattg gaacttcaag aactgtttgg tcagttataa ctgggaatcc
    17161 taaagctatt gctccagcac ctgcagatac aactaattca cttaatggtc caaatgcatt
    17221 aacaaatgca ggaactctat tagctgtgta ctctaataat ccgtttaaat caccaggagt
    17281 taatccaccg aatattaatg ctgctcttac tgcaacagat acaacgtgta taacagaagt
    17341 tacatcatat cctagaggta taactcttag ctcaagaccc atttttactc cagcttctat
    17401 agcttggtct ataactttac caactaagaa agttagtaaa cctttagatt ggtaatcttt
    17461 tataactttt gctgctgatt ctgcatctgg acactcacca agaacaactg caacaccagg
    17521 tatatctcct gtaactaatg gtacaccaag tgatctgatt atagggtcag ttatatgacc
    17581 tgcacatggc tcactatatg gagcatccat agttgaatat tttaatgctt caattacttc
    17641 tgctgctaaa gctgtagcta aaccagcatt aaaagcatgt tctagtaaat gagttctatt
    17701 tattaaagat tttactactt ctaaagcacc ttctaagtcc cctaaagtgt tcatcttttg
    17761 tcctgttgca gcatatatac aaggtaatga atatgctgta tcagggaaag caactttatg
    17821 ctcttttcca ttcttttcta tagcttcagc taacatagct tgagccgcac ctaaagcttg
    17881 ttctgaccct gtaaagatta tattatatag attcattcta tttccccctt taatatttac
    17941 acctattttt tattaactat accctaaaaa ccctagaaag caaatccacc ttcatttaac
    18001 tctaagaaag agtctgcata taaagatgcc ccgttgatta tgtcacaaga ttttacagtt
    18061 ttcattaatt ctgggtcaca tggattcatt atagctgaac taaatccatt agccattgcc
    18121 attgctaaga atgcactatc taaaacaggt ctaacatgct taggacatcc attagatacg
    18181 ttagataatc ctcctgtagt taaaagtccc atctcagtca tcatttttat agcttctaaa
    18241 acttctactt gtttttcttg catacctttt ataactaagc ataatgggtc aaatagtata
    18301 tcagttggct ccattcctaa cattaagcct ttttctaata tttcttggca gtaagccata
    18361 cgctcatcat tatctcttgg gataccttct tttgcacata aacctataac cattgcttcg
    18421 tattcagctg ctatatctat taaatcaaat cttgagccag catcagcaga gtttattatt
    18481 ggttttgcat tagttctatc ataaacttta agtccagctt cgatagcttt tttattagct
    18541 gtatctaatg ctataggaac attgttgaac tcagattgaa gtaatttgat tccccatgtc
    18601 attatttctt cgccatctct ttcggcaggt cctatattaa aatctatata gtgtgctcct
    18661 gcttctaatt gctcttttgc tctttttaag attggagctg ggtctctttc tgctaatgct
    18721 tttcttattg aaggtgatat acaatgtatt ctctcaccta taatcataaa tttttccatt
    18781 tataatgtcc ccctttaaat ttaatattaa tagttagtta atattttatt agtttgtttg
    18841 gcatgctttt tcttggtatc cttttaagaa cttaggtaat tccatagatt cctctggtcc
    18901 tataactaca ttccatcctg gtaagttatc ttctatatca cctttaagta ctgcaacttt
    18961 tcctggaatg ataaggtctt tacaattagt tacttcttct accttacttt ctttaatgaa
    19021 agcagagatt gagtttccac caaatttacc agcagcccaa gatgtaagaa ctgaatatcc
    19081 acctgcatct ggtattacta accatactgg aacttttgat ctttctatat caccagcaac
    19141 tatgaagtaa gttaatgcaa agtcaacagt aactaatact ggagaatttt catctggatt
    19201 gttgattgga taaatctttg gttcaactct cattggtctt tgtgggtcag tgtatatgtt
    19261 ttgtcttagt gcaaataatg gtaatgcctt agcataacta atatcatcta ttactattat
    19321 agaaccgtat tttatagtga atattgatga taaagcaact tccatcatag ggtttgaatt
    19381 agataatcta tttgcaaata ctattgaagg atatccaaat gttctatctt gctcttttaa
    19441 agctgttctt ctaacttgta tagcattagt gtaagtatct tttatgtttt ctcctgttac
    19501 atctaatact aattctttat atccagcggc ttgaattaat tcaactgttt catataattc
    19561 ttctaagcta ccagctttta cacctaatgg taaacttgct cctttaacta cttctatcat
    19621 atctttgtag ttctctttag ttgctccata taccattggt ctagcatctt ttaatacttc
    19681 aacagcttct ttaactactt gtgcatcatc acaagctaaa atataagcta cttctaatcc
    19741 actgcctttt attttatcta ttaattttaa atacgcatct ttatctccac aatactctaa
    19801 aacagccatt tcaactttca tttgttcacc aattcttaca tagtcaactt ttttcatgtt
    19861 agctatttta gcatctactg cttcatcact catacaagta cagaatgaaa ctgcatatct
    19921 atttctactt actaatgttt tttcatgtct aaataatact gtttctccac ctagttcata
    19981 ctcagatgca ccagcaccaa cttttaaagc tttcattaaa ggtgcagtag cctctgataa
    20041 ttttgctata gcgtcatcag acatatgtgg gcatttacct acttctacag ctcctgaagc
    20101 aaccttcata gagaaagcca tacaagttgg gaatccacaa tccttacagt ttttctttgg
    20161 tgttaattta aatatatcta aagcttttaa tgccattatt ttttccccct aaccttaaca
    20221 atttaattaa gctaatgcgt taaccaattc ttttatagtt tctattgatt ttggatgacg
    20281 aagtataact gcgtttgaac cacctactaa aacacttgct gcagttgaaa cttccattgc
    20341 tatagttctt tcttctggac aaccccaatc tggctcatct tctattggag ctatagcttc
    20401 tttaacatga cctacttcaa aagctactgg tgttatgata ggcatttgta atgtcttatc
    20461 attttgacca aatgctgcaa gtctaattct atccatagta gatgcaacgt actcatatcc
    20521 ataaccaact gctgaacatc caacattcat aactatattt tcacctttaa cgcctagttg
    20581 agttaacaat acgtttaatt gtttagctag gtttatatca acagaagatt cagctcctac
    20641 tttatgtgca taagccatac cagctgatgc tcctactcct ttgtagttat cttctactgc
    20701 tgacataaat aagcagttat gtccatccaa tgtttgagct aatttttcaa ataatttacc
    20761 atctttttca tgatttcctg aaccagctac aactaaaggt aattttattg cttcaattac
    20821 tgccttagct acatcagcac attcatcaac tgacttatct aatccatttg ggtcagaacc
    20881 atcaaatttt aaacaaataa aatctgcttg tgtattagct tcaacatatt tagcccactc
    20941 tactggacaa ttagctacat ctttgtataa ttccttatat gaatcagtcc agctttcagg
    21001 ataaacgtct gatatttgta tacctatttt tggagaatta cctacttctc catcaaaact
    21061 ataaaaaggt aatacatttt ccccacctaa tttaattgct ttttccccta ttcctacttc
    21121 aacttctgat atttttccag aatatttttg agtagacatt ttaaatgcca tatttttttc
    21181 ccccttaagc ctattttaat tctaatttag caaaaatttc tttcatagcc actttagatt
    21241 ttgaatcttc aggtaaatta actaaaggaa tacctgtaga atcatattca tatatcaatt
    21301 catccatagg tacaacaccg attagctcta ggttatgctt ttcaatttct tctttaacac
    21361 catcattcat aaccccattt ggaactttat ttacaatcaa aagtattctt ccaacactta
    21421 actttagttc ctcagctaaa tctcttattc tagcaacagc ttgtatgcta cgtcttgagc
    21481 aatcactaac caataaaagt gtatcaacat gtctagtgac ttttctactt agatgttcca
    21541 taccagcttc gttgtccatg actaagtatt tgtaatgacc agatattttg tttacttgtt
    21601 ctctaagtat cccatttaca aaacagtaac atccttcacc ttctgaccta cccattacta
    21661 ataagtcata tccttcgcct tcttcgatta tagaatttaa tctaaattga agatattgtg
    21721 cctttgtcat accaccaggg aatgcattgc ctaacttttc tctttgattt acttcttctc
    21781 ttatttctcc tattgttgct tcaacttcga tacctaacac ttcatttatg ttagcattgg
    21841 catcagcatc tacaactaat actggtccct ttttgtcctt aaccaaataa tcaatcaaaa
    21901 gccctgtgag acttgtttta ccagttccac cttttcctgc aacagctata ttgtatccca
    21961 ttttaagact tcccccttaa atctagccac agagccttat gtactctgtc tgtctattat
    22021 aatttaggta cagaatgaac acattctcca tgtacatcat gaagtgcttc cattagccct
    22081 tcagataaac ttggatgtgg atgtattgca tcccctactt gttctactgt taatcctaga
    22141 tgaacagcaa gtgacaactc tgtcaataaa tctgtagcat gaggtcctac tactgctgca
    22201 cctattatct tatcagtttc tttgtcagct ataactttta caaatccttg gaaatgtcct
    22261 atagcttgtg cttttccaag acctctaaaa tcaaattgac ctacattgta ttctacacct
    22321 tcagcttcaa gttgcttttc agtcttacca acaccagcaa cttcaggttc agtataaaca
    22381 catcttggaa ttgctctata atctactacc ttagtcttac ctaaagcatt ttcaactgct
    22441 acgatacctt ctttagaagc aacatgtgct aggaaaggag tatctattat atcgcctatt
    22501 gcgtatatac cttctacatt agtttcaaga tgttcattaa ctactacttt tcctctttcc
    22561 atttcaattc ctatatcttc tactccagaa ttgtcaaggt taggtcttct accaacacat
    22621 actaatgcat attgtgcttc tataaccttt ccattagaaa gagttgcaac agccttacca
    22681 tcaactactt cacaagtttg tactccaata cctgtaataa ccttaatttt atctttctta
    22741 aattgtctaa gtagttgttt agctacatct ttgtcttcat ttaatagtat ttggtcaacc
    22801 atctcaacta tagttacttc tgttcctaag gctctaaaga actgccctat ctcacatcct
    22861 ataactccac cacctactat taacatagac tcaggtatct cttcaagtcc aagaacttca
    22921 tcactagtta ttacaacttt tccatcatat gggaacattc ttggtacaac tggaacagaa
    22981 ccatttgcta gtattatctt atctgctttt atagtttcta ctgttccatc atctttagtt
    23041 acttctatag tgtttttatc aattaatttt ccaaaaccat taactaagtt tactcctctt
    23101 ttttcaaata agaactctat accacttatt aattggttta caactttatt ttttctttcc
    23161 attatggcag tgaagtttgg cttaattgta ccatctattt ctataccaaa atcctttgct
    23221 tcttttacag tattcaatac tcctgaagaa gcaagaagtg ctttagttgg tatacatcct
    23281 gcatttaagc aagttcctcc aactcttctt ttttcaacaa ctgttacatc tgcaccaagc
    23341 atagaagctt ttatagctgc tacatatcct cctggtccac cacctactac tactatcttc
    23401 atctttaatt cccccaaacc taatataatc tacaatcttg caatactata aacctgcggc
    23461 atccagtata gcaggtatat tctcttctgc cccaattgcc attatatgaa caccatcaca
    23521 taaatcttct tcttttaatt gtttgataag ttcccctgcc atttttattc cttcacttac
    23581 ccagttttct tttccagctg ctcttaatct ttcgatttgt tcatctggaa caaatattcc
    23641 tggtacattt gcagtcataa atttagccat tcctggtgat tttaaaggta ctattcctgc
    23701 taataccttg caatccatat cccttgttaa ttctctaaac tttctcattg tatttatgtc
    23761 ataaagtgct tgtgtttgga aaaatttagc ccctgctttt attttctttt tcatttttaa
    23821 taattgaact tctattggag aatattctgg agttactgat gctcctagat aaaaatctgg
    23881 agaacctttt aacttattac cagccatgtc cgtacctgcc attaaagtct cagcagtttg
    23941 aagtatcccc acagaatcta aatcaaacac tcccttagct tgtggatggt ctcctacgct
    24001 agtatggtct cctgttagtg ccaacagatt gtttatccca aatacacctg ctgacaacat
    24061 ttctccttgt atagctattc tatttctatc tctccctgtt atttgaataa ctggctctaa
    24121 tccagcatct tttaataatt tacatgtagc aagagaagtt gctctcataa ctgccgattg
    24181 aaagtctgtt acatttgctg catgaactct tccaactaac ggctttgcac attctattaa
    24241 atgtgaaagg tcagtccctt ttggaggagc catttcagta gtaactgcaa attttccact
    24301 ttctaatgtt tctcttaata agctcatctc taatccccct tttaaccttc taaaaaaact
    24361 tttatgaatt tgctgctgct tctttctttt tctttgcact taaacttctt ggattatttt
    24421 gcttagaata gtcttttggt ggtcttattt ctgctaagtt atctaattgt cctatagctt
    24481 ctagtctttc atatatcttt atccaagcac aatcattctc tgagtttact tcacacttac
    24541 catttttagc tccaccacaa gccccattca taagaccttt agcacacata gttactggac
    24601 atatcccacc tgtccaacca agttcacagt ctccacaagc cttacaggct tcttcatatt
    24661 ctccaactct ttgtacttcc cctataaaca aagtattgtt ggctggataa actggcttat
    24721 cttttaaatt cttaactatt gtctgtgtac catctccaca tgctaaagat aaaactgcat
    24781 cagcttcctt agtctcttct tttagagcct ttaaatcctt tttagacttt aaaaggttac
    24841 aagcagggtc aagcattaca tatcctaaga tttttttgcc ttcaccttca agagtttcct
    24901 tcatttttaa aacttcttct tccccaccac ttttacaagt tgctgcacat tgattacaac
    24961 caactaaaac cagcttgtca aaatctttta agtaccctag cacttcttct aatggtttat
    25021 tttcagaaat tatcatatct acttcccccc taaaccttcc acatatatat atctatttat
    25081 tgttttgtaa tttgcaagcc ttaactacat gttttgcaag tatagatgtt gtaactgaac
    25141 caacccccgc tggaactgga gttatcattg atactttgtc taaaactgca tttgtatcaa
    25201 catctccaca taaatttcct tcttcatcaa catttatacc aacgtctata actaccgctc
    25261 catctttaac aaaactttca tcaaccatct tagctctacc aatagctgct attagcacgt
    25321 ctgcctctgc tgctacacct gatagatttt tagtttttga atgacatatt gtaacagttg
    25381 cgtgttcact taataaaagc attgatactg gctttccaac taccattgat cttccaagaa
    25441 ctgttacctt acttcctttt aaatcaacat tatagtgttt taatatctca actactgctg
    25501 taggtgtaca tggtggaaat ccacttttat ccccttccat taccttagca gagtttattg
    25561 gactaaaaca atctacgtct ttttccggtg caataacata ttttataact tcttcattta
    25621 agtgttttgg aagaggtcta aaacataata taccattaac atttttgtca tcattaactc
    25681 tctttaatac atcaatatat tcctcttgtg ttatatcctc agctaactct aacacttctg
    25741 ttgttatacc tatgttttga catctcttta gtgctcctct ttcataagat aaatcatcac
    25801 ttcttgcccc aactctaact attgttagtt taggatttat accttcttta attagtaaat
    25861 cgacttcttt tattaattct tcactaatct tatcagctac aggttttcct tttataattt
    25921 gtcctttagt tgacattcct tccatacatt cacaactccc ctacaagcaa acttaaatta
    25981 atttattcta tataccacat attaataaca tagtatctac tttcgtcatt taaaaattat
    26041 tttccaagag ctttttcaac tttggcataa acttcatcac aaatttttac accatcttca
    26101 actaacttat ctacttctgt ttttacctta ttaacatatt ctttatcttg tatagaattt
    26161 atatttataa ctacatttaa ttgtccacca agtatagcag ctcttaaaca ctgaactcct
    26221 acaccaacat cacttatagc aagtcttgaa cctttatcaa ctaaatcttc atgtaactta
    26281 atagcttcat agcaaactct tactatgttt aatggtactt cacaagcaac ttttaatgcc
    26341 ttttccattg tttctgcttt tattcttttt tcttcttctg tttctttagg aagcccgtaa
    26401 gcttttgata atggtaagaa acattctgca tcatcatcta tcatttttaa taaatctttt
    26461 tctaactttc ctgccttact taatatctct tttacacttt cttcatactc agcatacttc
    26521 ttcttcccta ttgtaaggtt acataccata ctacctaaag ccatacctat tgcacctact
    26581 agtgcagcag ctcccccacc acctggtact gcttctttgg aagatagtac ttctacaaaa
    26641 tcaacacaag ttttatctgc tattttcatg ataatcctcc ttgaaaggat gtaggaaaat
    26701 atctcaaatt gttcgagata ttttacgaac tattttaaga tatctcctac tctttattta
    26761 taaattaatt tccttagaat aatccagcta ttactccatt ttcatcaaca tctatttttt
    26821 ctgctgctgg aacttttggt aatcctggca tcttcattat ttctccagta agtgcaacta
    26881 taaatccagc tcctgctgaa attgttgctt gtcttacagt tattctaaat cctgttggtc
    26941 tacctaattt agtttggtca tcagttaaag aatattgagt cttagccata catactggaa
    27001 ctttagtaaa tccaagtttt tctaagttag ctatttcctt atcagcttca ggagtataat
    27061 ctacaccatc tgctccataa atcttagtag ctatagcatt tatcttgtct tttatagata
    27121 aatcatcttc atagcagaat ctaaagttat tttcttcttc atccaataat cttaaaactt
    27181 ctttagctac tgcaattcca ccttctccac cttttgccca tacttcagaa agagcaacat
    27241 taacacctaa ttccttacac ttgtcttcaa caagtttaag ttcagcttca gtatcaagtg
    27301 ggaatctgtt gattgcaaca actgctggta atccatatac ttgagttatg ttttcaacat
    27361 gttttaataa gtttggaagt cctttttcaa gagcttctag gttttcgtta tttaagtctg
    27421 ctttagcaac tccaccattg tattttaatg ctcttacagt agcaactatt ataacagcat
    27481 ctggttttaa gtttgccatt ctacatttta tatctaagaa tttttctgct ccaaggtcag
    27541 caccgaaacc agcctcagtt attacataat ctgcaaagtg catagccatt cttgtagcta
    27601 taacagagtt acatccatga gctatatttg cgaatggtcc accgtgtaca aatgatggtg
    27661 ttccttcaag agtttgaact aggtttggtt ttaaagcatc ttttaataat gcagccatag
    27721 caccattagc ttttaattgt ctagctgtta caggttcacc ttcgtagctg tatccaacta
    27781 ttatgtttcc taatctttct tttaaatcag atatatcact tgccaaacag aacgctgcca
    27841 ttatttcaga agcaacagtt atatcgaatc catcttgtct aactgctcca tcaccttttt
    27901 tacccatacc atcaacgata tttctaagtt gtctatcgtt catgtctaca catcttctcc
    27961 aagttatttt cttagggtct attctaagtg catttccttg gtgaatatgg ttatcaagca
    28021 tagcagctaa aagattgtta gcagctccta tagcgtgaaa atctcctgtg aagtgtaagt
    28081 ttatatcttc cataggaaca acttgtgcat atcctccacc agctgctcca cctttaacac
    28141 caaatactgg tcctagagat ggttctctta aagcaactaa tacatttttg tctagtttag
    28201 caaatgcatc agcaacacct atagtagtag tagtttttcc ttcacctgct ggagttgggt
    28261 ttatagctgt agttagtatt aacttagctt ttttaccagt ctctcttttt agtagattgt
    28321 agtcaacttt tgctttgtat ttaccataaa gttctacatc atcttcacct agtcctaatt
    28381 tttttgcaac ctctctaata tcttgaggct tagcttcttg agctatttca atgtcagatt
    28441 taaatcccat aactgaatct cctcccacaa cctaaataga tttaaaatat aatttttgta
    28501 aaaattgtat taatatgtaa aaattatatt ttataagata attatacatg taaaattact
    28561 taaaaactag ctactaagct attaaaatat tcaaacttaa tagctagtta tttatctaat
    28621 tacttactta ttacctatct attgcctatg tctaaccttt aaccaactta aaatcccaaa
    28681 tcaaaatttc tatctatttt atttatcctt aagtgacatt aatttatctt ttattgcttt
    28741 aatctgttct ttcgttgttt cactagaatc atatggagat tgattagctc tatcagaatc
    28801 aataacatct tggttataat aaataaatcc taaagattct ccatcttcta agttttggat
    28861 tataaattct tcatcttcct tatttctaat tttatttcct actacaaata ctttatttac
    28921 acctatatca tgtccaagtt tcttaacttt tctataagtc tgtagacttc tctctcctgg
    28981 ttctacaact actataaaag catctacacc ttgggctgtt cctcttccta agtgttcaat
    29041 tcccgcttcc atatccataa ccactacatc tttattttgt agtattaaat gtgagcagag
    29101 tctcttcaaa agaacatgct ctggacatac acatccagta ccaccagaat ctacggttcc
    29161 caatgtaagt aatcttactc cattgtattc tttacagaaa ttttcaggga tatcgtctac
    29221 tttaggattc atcttaaaca tttttccaaa tgaacctaca gaagcagctg ttctatctga
    29281 aactaatttt ttcatttctg atataggtac tattgattca tatacttctt ttggaaaacc
    29341 tagtgctaaa gctaaattag catcagggtc ggcatcaaca gcaacaactc tgtatccgtc
    29401 ttcagcaaac atcctagaga gcattgaaga aaaagtcgtt ttacctactc cacctttacc
    29461 tgttattgct atcttcataa tccctaccac cttttattac tatttgcttt ttagtagtta
    29521 attttacagt taaatatttt ttatactcta tatttttata cactaaaaat aaactacagc
    29581 tagtccttac aggaaccaat taaatttacc ctactagctt ttcaaagcag catgtatagt
    29641 tttattattc tttaatattg tttatatttt atttattcag tatattcatt atattttgat
    29701 gttctttact ttaatatttg actttattca actttattca accagaaact tcttataaaa
    29761 acgtttattc tataacttca ctatttttta agcttcagcc ttttctgcca tcttttcttc
    29821 aactaatgct tcaaagtgaa cacgtttttt ctctatatct tctattattg tagctgctaa
    29881 ctcttttgga tttgtattta cagtaaatgt agctccaact ttatctttta attcactagt
    29941 taatatatcc actgctcttg aagaaccagc tattggaggc attattccta aataagtgtc
    30001 tataccagaa gctacaacat agcatcctat agcaactgct ttttctgaca tccactctgg
    30061 agctatacct acaactggaa ggtcacacat atccatatct aagtaattag ctgctgctcc
    30121 aactaaatct agtatacgac ttatatcaac acaagaaccc atatgtagta ctggtgggat
    30181 tcctactaat ttacagacag ttgcaagacc tttacctgca tatttttcag ccgcatcaga
    30241 ttccattaaa ccaaacttag ctgctgctga agctccacaa cctgtagtaa ctactattat
    30301 gtcgtttttg attaattctt tcattattgt tacgtgagcc tcatttgaaa ctcctttagc
    30361 attgttacat ccaactacac cagctgctcc tcttaatact ccagatttta aacagtctgt
    30421 taatggctta actgttcctg ctgggtctat atgagagttt acaactctgt ctaattgatt
    30481 tactactgct tctacactat acccaactgt tgcgcctgat tttaattcag gtataagtac
    30541 ttttgattta tctctatttt taaagtttaa aatagcttct ttaactattg ctttagcatc
    30601 atcaagtgct tgttcttctc taaattcaat ataagttgaa cctgttatct tagcttttgg
    30661 agaagttgtt acaaatttag tatggtaaca atctgcaact ctagctaatg cagggaatat
    30721 acattgaacg tcaactatta cagcttcaac tgctccagtt attattgcaa gttcttgttg
    30781 atggaagtct cctgctattt ttacaccatg tctcattgta atctcattac ctgtacaaca
    30841 catacctgct aagtttattc catcagcacc aacttcttta gctaaagcta ctaagtctgg
    30901 ctcttctgat gctaaaacta tcatttctga taatgctggc tcatgtccat gtaatattat
    30961 gttaacttta ttttcttcta atactgctaa attcgcctct gttcttcttg gcactggagt
    31021 tccaaataag atatcgctta gacgagtacc tatcatagaa ccaccccaac catcagccat
    31081 agaagttcta agactcatgt gtattaaact atctatatca ccagtacatc ctatatgagt
    31141 agagtgcatt attgttgcaa tttctctatc tatagctctt ggctctatag cgtattcttt
    31201 ccatattttt tgtcttggtt ctggtgcatt ttttataaat cttaatgtcc caaaaggttt
    31261 tccaaattcc attaatgcaa tttctgccac ttcatgtgct acatcataga tatctctacc
    31321 ttctgtctct acatcccatt ctttagctaa agtaattaac ttagcttcgt ctcttatttt
    31381 atagtttcca tttggatcag ctaatgctaa tgtatgtgct atatctctag catggtctga
    31441 atgggcagct gtcccaccag caaccattct tgcaaagttt ctacctacta tagtatgctc
    31501 atctgctcca cataaccccc ttggagtctt agggcttatt ctacatggtc ccattccaca
    31561 tattctacag caaacacctt gaagaccaaa accacattgt gccttaaagc cttcttttct
    31621 atcatacatt gtttctacgc cttcttctct agctttagct atcatcttct ggctgtttaa
    31681 atcaattgtt aacatttttt catccatatt cctatcccct ttttttgatt tttaaaatcc
    31741 cccaggtgac tgacttcatg tcataaaata ataaaataaa tgctttctaa gttgattata
    31801 tactaatttt cagtgctttg ctttagattt aaatattttt ttaacaaaat tagataatac
    31861 ataaaaattt tattttctac acttaattta agttgatttt atcgcttgtt tttggctatt
    31921 tgttaatatc taaacctact tttttgatac ttcagtttaa ttaaattatg ttttgatttt
    31981 tttaaatcaa atttgaaatt tttctagtca tttttatatt attgcagatt gtttgtgtat
    32041 ttattatctt tcactactgt ttggacttat tacagatttt tactccctaa acttaattag
    32101 taaaaacttc ttagaatagc attttttgga ctaatttttt tgaatttttt tatttttcct
    32161 actttaatat taattatagc ttcaaattga acatttgtat atattcattt gaagctataa
    32221 ttatttttta tataaaactt ttttaataca gcattaattt agacattttg actatttttt
    32281 ctactttaat tcatttattt caagcgattt atcctatttc atatttttag ctacaatagc
    32341 cccatatgca atagaatata atttagattc atgtgaatca gctcttgaca ctaaaactat
    32401 aggagctttt gcacccataa ttatacctgc tgatttagag tctgccatat atgtaaaggc
    32461 tttccctatt ccattaccaa cctctattgt tggaaccaat atgatatcta catctccaga
    32521 tacttcactt ttaaaccctt ttatctcact agcttctttt gaaacagcta aatcaaaagc
    32581 cagaggacct tctacaatta cattttcacc aaattctcct tctttacaag cttttgctaa
    32641 catatctgca tctacagttg cttgcatctt aggatttact ttctctttag ctgccaaaca
    32701 tgcaactttt acagtttcca ttcctaaagc ttttgcagct tttatagaat tttttaatat
    32761 ctttgccttt tgttcataat caggagctat attcatacct ccatctgtag ttataagtaa
    32821 tttatggtat ttttctaact catataccat aacatgactt aataaactat cagttctaag
    32881 accatagtct ttatttaaca cttcttttaa tatgacagat gtatctaata atcctttcat
    32941 tacaaagtcg gcttctttac ttgatactaa tttaactgct atttctgcac actctttgat
    33001 atcatcctta tccactattt tgataccact taaatcaaat cctatttctt ttgatatttc
    33061 tcttattttg ccttcatttc caaccaatat aggagtaatt atatttcttt ctacagcatc
    33121 ctttattgcc attaaaactt ctttatcatg agctgctgct acagagagta caattttctc
    33181 tcctccctta agttgttcta aaatatcatc taattttttt atcaaaagta acccctccct
    33241 tatatatatt taactaaata ataccatatt tgctatacct tttctcaatt tattttttat
    33301 gttataaaga atttatataa ctaaaagaca gtttaatatt ttattctggg atttcatcaa
    33361 taactcttaa aagctttgca ggaacaccac ctacaatagt attttctgca acattttttg
    33421 ttacaactgc tccagcagcg ataattgcat tatctccaat agtaactcct ggaactacag
    33481 ttacattagc accaatccat acattttttc ctattgtgat tggagaagga taagttgtac
    33541 cacgtttctc tggtgcaata ccatggttaa gtgtagccaa taccacatta tgcccaattt
    33601 gcgtattatc tccaatctca atccccccct ggtcttgaaa atggcaactg ctatttataa
    33661 aaacattttt tcctattgtg atgttttttc cacaatctgt ataaaatggt ggaaacataa
    33721 aaaatgattc atctaccttt tttccaatta actcagaaaa taaactacgg acttcttctg
    33781 gttcatgata agaagaattt aacttacttg tgattttcat tgcttcatta ctcaacttaa
    33841 ccataaattc atgtatttct gatttactag aaacaggttt tctttgattg atatgtgtta
    33901 aaaattcatt gatattcatt ttatacatcc tttcaatatg tgctttaatt tatttgtaca
    33961 ttctaaataa tattttaata aaaattaatt taatttattt ttattaaaat atcattgctt
    34021 cttttagtgt aatatacaat tatatctata tcaaatatct atagtaaata cttaactata
    34081 cttaaaaagc atataaattg atataattat ataatagata agttaatatt gaggagggat
    34141 aattatggaa ataagagtat taagatattt tttagcaatt gcaagagaag aaagtatatc
    34201 tggtgcggca acctttttac atataacaca accaacactt tctagacaaa ttatggaact
    34261 tgaagaagag ttgggagtta aattattggt tcgaggaaat agaaaaatca ccttaacaaa
    34321 tgaaggtagg cttttacata aaagagcaga agaaattata tcattagttg ataaggttga
    34381 attagaattt gacaatccca atgagattat tagtggtgat atatatatag gaagtggtga
    34441 aactgaagct atgcgactta tagctagaac tgcccatcat tttcatcaaa aataccccaa
    34501 tgtaagatat caccttttta gtgggaatgc tgatgatgtg actgaaaaat tagacaaggg
    34561 actgcttgat tttggtattt taattgaacc tgcaaatata cagaagtatg attatgcaca
    34621 aattcctgct actgatgtat ggggtttact aatgcgtaaa gatagtgccc ttgcatcaca
    34681 aagcacaata aatccaaaag atttatgcaa tatacctctt attatttcaa gacagacact
    34741 tgttgataaa gcaatttcta aatggcttca agacagtaat gaaaaactaa acattattgc
    34801 aacatacaat ttagtttata acgcttctct tttggtagaa gaaggtctgg gctacgctct
    34861 gtgtttagat agactcgtca atactacaga aaacagtaat ctttgtttta gacctctatc
    34921 tcctccttta gaagctaaat taaatattat atggaaaaaa catcaaatat tttcaaaggc
    34981 agcagaaaaa tttttacttc aaatacaaaa agatttttct tctatcatgt tggatacatc
    35041 taagttggat acacctaaag aaaaataaat cataataaaa ggtgagagtg tctcaaaatg
    35101 aactttgcac tgtataaggc actctttttt atacaaagta aaatatatgt tcatcatttc
    35161 agcactaaaa aagaggctta tctctatttt gagatagccc cacttaacca ataaaattaa
    35221 atacaactta acaatatcct tacttattta ttctaagttc tcttagacaa gcaatagtaa
    35281 tttcatcttg tggatttaat tcataagctc tttctatata gtaagttgca tcatcaatat
    35341 caccattatt taaataagcc attcctaaat tgcaaagtat ttctgggtct tcctttatct
    35401 tagccgcttg ttcaaaatac tctattgctt tatctaaatt attcttataa gcctcacaaa
    35461 gccccaattc cactatagta tccacttgat taggctttat tatcaatatc ttctcaagat
    35521 acatcttagc ctgctctatt tcgcccatac ccttatatcc aagtgctatc ataaacagca
    35581 agttccacca atccatatgt tcttcttcta atggaagtaa cttctctaat ccttccttaa
    35641 acttaccttg gaatacaagt gtatatcctt cttcatattg aactttaaag tccatcttgc
    35701 ccagattctc ttgtacctca gctactaaat cagcatctaa ccctagcctt aacgcttctt
    35761 cccaagtcag cttagtcttt aaatattgac cttgattata ataatggtag cctaagtgat
    35821 aataacctaa tgcaaaattc tcatcaacat caattacctt ttcaagctta gccatggctt
    35881 caagtaagaa tgcattcatt gccttatcat ccatatcttt ttgatactgt ttagctatct
    35941 cttggcaaac aatcgcatag ttatacattg catctaaatc atctggatac attgttataa
    36001 atgcttttat atatattaaa gaatctgtat gttcaccaat ctcaaagtat tttctcgaca
    36061 tatatcccat atacgatttc aaatccaaat caatatcttt tgataaagca tttaaaaact
    36121 tctcatactc tgcattgttt ttaaacttac tatctattcc aattatatat atcattgcat
    36181 ctgctataga cattgaattt aacttatctt gagcagtctt ttcttttatc ccttttacta
    36241 atacctcatt ttttataggt acatctaatc caccttttgg cacttcatag ctttttaatt
    36301 taaactctcc accatgtttt atagttataa aagctaattc ttctgctttt tttagcaaat
    36361 atttttctat cttaaaatcc attttatcct cctaaatact taaatctcta ctttatgagt
    36421 tttagatttc tcaaatattt ttttatcatc atactcaaat aagactacat tttctacttc
    36481 tcttatttct gtatttatat cattttttaa atccacaaca ttaaatctaa atggctcaaa
    36541 atgaacatat tttattatat gtttagctgg tttatcaaca aaactaggta aatatgttga
    36601 taagttatca ttatcttgta aaaatacatg gcatctattc ttaaaatcat ccacatctag
    36661 cttattaaaa aactgtggta tatttgatgt tttacccagt gatatatagt catattttaa
    36721 tatatcgttg aatacatcat tatctatgtt gattttttca ttatagaaat ccatcaatat
    36781 tttatataac tgattctttc cttgtgccaa atcaaagtag ccattttcat caaaatactt
    36841 ggcaaaaacc tcaaagaatt taaaagggct ttttttataa aatctgccta taatatatct
    36901 catagataac acaaagtttt tagaattgta gtatctctcc agtatatctt ctatatcctt
    36961 tagcttcaaa gtttcttcat agcttatata attattgtat aaaaactcat atggagcata
    37021 atccttatat ttaaaaccat gctcatctgc aacttttctc atacctgtcc ctttaatcat
    37081 ctttaaaaag cctaattgca aatgctctat acctagatta aatacatcat taaaagaatt
    37141 ttcaaagctc ttataatctt cataaggcag tcctgcaatt aagtctaagt gctgatgtat
    37201 atttctataa gatgctacag tttgtacaac atgagacaac tttttaaaat catcccttct
    37261 acctactgca tcaagtgttt tttcattcgt tgactgaaca cctatttcaa attgaaaaag
    37321 accttcttta caatcagcca aaaactcaag catcttatca tctatcaaat acgctgtaac
    37381 ttcaaaatgg taagttgtat atccattatc atttttcata aggaaatcca tgatttccat
    37441 agccactttt ctatttgcat tgaatgtcct atctataaac tttatctgtg atactcttgc
    37501 atctattaaa gcttttaaat cactctttat cctgtctaaa ctaaaatacc ttagacctgg
    37561 aatagtagat gataaacagt attggcagtt aaaagggcaa cctcttgatg attcataata
    37621 cactattcta ttttcatatt cttttggatt caagttttca taaggactag gaatctcatc
    37681 caaatcttga agtaattcca taggcttatt cactattatc tcattgcctt gtctatatac
    37741 aagtccattt acatcattta tttccatctt tccttgcaag tgaagtacta agtctctaaa
    37801 aattaattct ccctctccat aaagtatata atccacaaaa tcatactttt tcattgcctc
    37861 ataactatca taactcactt ccggtccgcc caaagctatt ttagtattag gtctaatttt
    37921 ttttagatta tcacaaagtt taactatatc tcccacattc cagatatatg ttgagaataa
    37981 tattatatca tattcatttt tatatatatc ctttaaaata tagtccaaat cgttatttat
    38041 cgtgtattct ttcatattaa cttctatcaa atctcttaca aattctttta gatatcttat
    38101 agccaaattt gtatgaataa acttggaatt taatgttgtt aataaaattt tcaaatattt
    38161 ctcacctctt tttaagatta tactgtcttt ttgtacccct tataggttaa aatagtatat
    38221 gtaaagtcta aataatatct atataattaa tttattaatt tcaagtaatg tgaggaaagg
    38281 aagtttttta tggaattaat tgaaaaagta tctatcaact ctttatcaaa aagagattta
    38341 ctacttatta taaagtctct tgaatacact aatgaaaata ctcaaattag tgatttcatt
    38401 gaccttagga ataatatagt aaaggaatta tgttttttaa ctgacactaa ggaaaaagat
    38461 tttttagatt atctagaaaa aaatagcaca ctctaataac ctaaaccttt ttaaacttta
    38521 atatataaat acctgattta gaatttattt ttatgcttaa tatattatca ttaaaggttt
    38581 tcgacataac tgttatttga ttaaatatag ggtctattat agtattacct tcttcaagtc
    38641 ttataagact tcttgggtct atgcttttat tatatgcctt aactccaatt ttatattttc
    38701 tcaatttaac aacatattcg gacatagaaa aaataaaatc tatattatct tcatttaatt
    38761 tactatcttc acccaaaatg gataatatag atttttttat gctattaatt ccttcatttt
    38821 ctacagttct atcttccata tatacatcat atagaacatt taaaacattt aataaaatac
    38881 tttcttcatc ataatcttca gatttcaaat caatgtactt atcaattaat tgtattgctt
    38941 taatccttgc catctgaaac tttacaacat ctgatttgtc taattctata gaaaatccta
    39001 ttatcttgtc tttaatactc tgtagtaatt cgacatactc tattttacta ttatttataa
    39061 gattgtgcat aatggaatta tacaccacag aacctaataa ataatcaaat aaaagttctt
    39121 cttttttaaa ataaataaca tactttttta ttagtttaat aacctcatct tcactttttt
    39181 gaatattagt atttgataaa attagtggta ataaaatatt taaaaaattg atatttccta
    39241 ttttttctcc ataaaatcta tagtaaataa gttcatctct cagtctattt tcgtctatat
    39301 acacacatct ttgcgtttta tcatctatta aggattttgt atttattata gttaaatctt
    39361 taagagccct acttgcacta ctaaaagaag atgtgtagac tttttttagt aattcgtcat
    39421 tcataaatat atcctttctg aaaaattgaa tgtaaataaa ttgttattaa aaaaaaagag
    39481 acagcccgtt taagaagctg tcatatatag ggggatagta gtgtacaaaa atcattgtaa
    39541 tctactatct atatattatt atacgatact acttttttat atgtaaagca tcagttttcg
    39601 acatccctat attttacagt tatacaaatt gcgtactaag ttcaatttta ttctttttat
    39661 ttcaacattt tatcatgcta cattctttca ggagcgtgca ttcccaacaa tttcagagaa
    39721 ttttctaaag tttgttttgt tgctaaaact aatgcaagtc ttgcttttct tatttccaaa
    39781 ttttctacta aaattggatt gtcatgataa aatttattaa acgcttgagc taaatctagc
    39841 ataaatctag tcactatatg tggctcattt ttctttagag caagtaaaat attcttattg
    39901 aatgattcta taactctaag aacttctgca ctatcttcat cagctaatag actataatct
    39961 atatcagaag tcacttctac ttctgctttt cttaaaactg cacagcatct agcatgtgta
    40021 tactgaacat atggtccagt ctcaccatca aaactaagcg ttctttccca agagaaggta
    40081 tagtctttaa ttctactatt agataactct tggaatacta ctgccccaac acctacttgc
    40141 ttagatattt catccacatt tttagcattt gggtttttag ctagtataat ttccttagtt
    40201 ttatctatag cttgatttaa agcatcttct aaaaatacta ctcttccttt tctagtcgac
    40261 atagtacctt cttcaagtgc aaccatacca aatccaacat gaattaaatc ttttgcccaa
    40321 tcatatccca taagctcaat taccttaaac cattgttgga aatgtaagtt ttgttgagaa
    40381 ccaactacat atatacactt gtcaaaatca taagtttctt ttctatatat tgcagcagct
    40441 aaatctcttg tcatatataa cgttgagcca tcattcttag taataagtgc tggtggcatt
    40501 ttatactctt ctaaatcaac aattcttgct cctttagact cttttaaaag atttttctct
    40561 tctaatagtt cgataactct gttcatttta tcagaataaa aactctctcc tgctaaagaa
    40621 tcaaattcta tatctaaaag tttatacact ctgttaaact cttttaaact ctcatttcta
    40681 aaccattgcc ataattcttg tgcttcttcg tcaccatttt ctaatttatt aaaccatgct
    40741 cttgcttcat cttccatttc tgggtgttgt tcagcttcat catggaatct tacgtataat
    40801 tttagtaatt ctggaattgg attagactct actacttctt tttctcccca ctttttaaag
    40861 gccactatta acttaccaaa ctgagttcca tagtcaccta aatgatttat tcttatagtc
    40921 ttgtatcctt gcgaatcata tatcttatat aaagcattac ctataactgt agttcttatg
    40981 tgccctatat ggaaaggttt tgctatattt ggagaagaat attcaactat tacagtttta
    41041 tcttttccaa aatcactatg tccgtaattt tctttttcat ctaaaacttt tttaattaca
    41101 gtttctgcta attgagattt atttacaaag aaatttacat atccgcctaa ttgtattact
    41161 tttgttatct ctccacttgg ttctatactt tccgccaatt cacttgctat catgtttgga
    41221 gcttttctaa acactttggc taatttaaag catggaaaag catagtcccc catatcctta
    41281 tttggtggaa cttctataag tgcctctatc tcttctttcg ataaatcttc tattttttct
    41341 ttaaggcaat ttgatattgc caccttaaaa tcttgcattc tgttgctcct ctctacattg
    41401 ttcctggttt atattgtgct tatctcttta tgttaaatca aatttcgtgt tttttcaagt
    41461 atttcattac agtgtaaaac actttagtaa ttatgttttc tgtatatatt aaaaatacta
    41521 taattgcagt ttttaatcac actattattg attttttata agactttttt ctattttatt
    41581 gtttcttgta ctatccagtt gactttatat acttttttta atcttttatc tattttcata
    41641 cgtcaatgct aattttatat acttttcttt taatctttta ttaattttta tacatcaatg
    41701 ttaactttat ataagttttt ttaatctttt atctgttttc atacatcaat gtcagcttta
    41761 tatacttttt ttactttttt atctattttt atacatcaat attagcttta tatacttttt
    41821 ttaatctttt atctattttc tataaatctt tttcactctt aacttttatt ccttcttctt
    41881 ctaatagcct tgctgttata cctttccctg gtatcttttt acctgaaaaa ctaccatcat
    41941 atattagatt acatccacaa gaggggctac cctcttttag tattgcttcg ctacatacat
    42001 ataaatttgc tatttttaga gtttcattag cccctcttac aaagttttct gttacatcct
    42061 tattatcttt agtaattact tttccatcaa ctatctcagc aggtggtctt ggtgtagata
    42121 gaccacctaa ctgctctggg catacaagtg taaattcttt gccttttaaa tattccttga
    42181 ctttttcatt atcattatta tcgccactat atttacaatt tattcctatt aaacatgcag
    42241 ataccaatat cataaaaatg tgctcctata caaattctta atatattctc atacaaatta
    42301 ctaatgtatt cttatacaaa ttattttaat ttaacaattg ttactcctgc tccaccttct
    42361 ccatattgac cacctctttg tgattttaca tgtctatttc ttcttaaaat gtcttgtagc
    42421 cctgctttta atactcctgt acctattcca tgtattactg ttacagactc taaacctgcc
    42481 acatatgcat catcaagata cttttctact tccattatag cttcttctag gtttaaccct
    42541 ctaaggtcaa cttcattttt tacactacca gattttgacc ttataatgtt tctcgttgat
    42601 ttagttacat ttgttgaaac atctttgcgt gttttttgta aagacttaaa aggtagagtc
    42661 atcttcatta taccaatttg cactactgct tcttttctct ttttatctac tgaaactaca
    42721 ctaccatttt gatttaaagt tattacctta acttcttcac ctggctttaa gtcttttatc
    42781 tccttatttg atacctttgg aactatcata ctttttactg ttggctgaag acttcccata
    42841 gagctggtca attcttttct aagttcttct atcttacgat tcttttcttt tgaagctctt
    42901 tcttgttcaa gacttctaag ttcttttatt ataatatcta cttcttcttt agcttgtctt
    42961 ataattgaaa atgcttctga tttagctttt tcaatcatct tatctctttg actgaccaat
    43021 ttttcaagct tttcgtcata ctctactttc agtttttcaa tctcttcttt taatctttca
    43081 gcttcttctc tatcttctac tgctttgatt ctattctttt caacattctg taacacatct
    43141 tctaaagcta tgttttctgt atttatatat tctttagcac gtgaaatcac ataatcactt
    43201 agcccaagtt ttctagaaat ctcaaacgca tttgattttc cgggaacacc tataagtaac
    43261 ttatatgttg ggcttaaagt ttctatatca aattcaactg ctgcattttc tacacctggt
    43321 ttagtaagag cataattttt aagctcacta tagtgagtgg tagctataca tttagctcct
    43381 acagaattta tatcttctaa tactgctatt gcaagggcag ctccttcaac agggtctgtt
    43441 cccgctccta actcgtcaaa tataactaaa gagtctgctg ttacgttttg taatatagat
    43501 actatattcg tcatatgaga tgaaaatgtt gatagactct gttcaatact ctgctcatct
    43561 cctatatctg caaatacatt atcatataca cacatactac ttccataatc agcaggtata
    43621 tgaagtccac tttgtgtcat aagtgcaaat agacctacag tttttattgt aacagtctta
    43681 ccacctgtgt ttggacctgt tatcaccaat gtatggaagt ctcttcctaa ataaattgta
    43741 tttgctacta ccttcttttt atccagcagt ggatgtcttc cattttttat gtttaaatat
    43801 ttatcctcat ttaaagttgg ttctattcct ctcatctgta tagaaagttt tccctttgaa
    43861 aatgcaaagt ccaatcttcc aagtatctct tgattagaaa ttaagtcttc actaacttct
    43921 ccaaccatag cagaaagctc tgataaaatt ctttctattt cttcttgctc acctagtttt
    43981 aactgtctca attcattgtt catctctact atagtcattg gctctataaa aagtgtcgca
    44041 ccagaagatg actggtcatg cactatacca gctacttgcg acctatattc agattttaca
    44101 ggtactacaa atctatcacc tcttagagaa attattgcat cttgtaagta cttttgataa
    44161 gtagttgaac ttattattga atttaatttt gacctaatgg attgattttt ttgggctatt
    44221 cttcttctga tatctctaag tgtacttgaa gcattgtcag atatttctac ttcactaatt
    44281 atagcattgt atatctggtc ttctatgtcc ttgtatacat atagagaatt tgatagagat
    44341 tgtattattg gataattaaa atcttcttca tcactactag ataagctatt tcttaatctt
    44401 ctagccactc ttaaggtatc tgctatcata atcagacttc ctgggtcaag agaagcacct
    44461 atataagctc tctttacctt atcttctata tcatgaatac cttccaaccc tactgaacct
    44521 ctctttataa taattgattg agcttcacta gtttcttcta gcatagattt cacttctaca
    44581 aaatcagtat ttggtactaa attttctata tattttagac ctaaagaaga tgaggctttc
    44641 ttctttaaca aatctattat tttattgtat tctagaactc ttaacgattt ctcattcatt
    44701 ttctatattc accatcctcg atattttaac ttttttattt aattttaaaa atgtatgttt
    44761 tcaagcattt ccaataaaaa ctgcaagttt tatttctttt tcatttctaa aattattata
    44821 ttttttctct tttagatata ttttacacat attttgtatt atactatgta tgtagctatc
    44881 tatatattat acataaacat gctttatttg taatataatc tgttaaaaaa tcttctttaa
    44941 tatttttaaa ggagtttatt ttagcttata aaaaaaaata gctacttata tagataaatt
    45001 aaggaggaac atgagtcatg ggaaacgtat tagaaggatt aaaaccagaa agtgttttta
    45061 aaaactttga gaaaatatct caaattccta gaggttctgg aaatgaaaaa ggcataagtg
    45121 attttttact tagctttggt aaaaatttag gtcttgaaac tattcaggat gaaagcttaa
    45181 acataataat aagaaagcct gctacaaaag gatatgaaaa ttgtccaggt gtagttcttc
    45241 aaggacatat ggatatggtt tgcgaaaaag aaaagaatgt tgaacatgat tttcttaaag
    45301 accctataaa attaagaatt gatggtgaca tgatatatgc tactggaact actctaggag
    45361 ctgacaatgg tatagctgtt gctatgggta tggctatatt agaagataat actttagagc
    45421 atcctgcact agaagtttta gttactgtta atgaagaaga tggtatgaat ggtgcagatg
    45481 cactagaccc tagcttaatt aaaggtcaat atatcctaaa tatggactcc gaggaggaag
    45541 gatacttatt agttagctgt gctggtggta aaacttgtgt tgttagttta cctgttgaat
    45601 acaaggaagt taaaggtgac aaacaaggat tattagttga ggttactgga ctattaggtg
    45661 gacactctgg tatggaaatt gttttacaaa gagcaaatgc taacaaagca atagctagag
    45721 tattaagtgt attgaatgtt gattatgaat tagcttctgt tgatggtggt actaagcaca
    45781 atgctatacc tcgtgaagct aaatgtgtaa tagctgttaa taaagctgat gttgaatctg
    45841 ctaaaaaaca aataaatgac attttaactg catttaaaca tgagtttact acttctgacc
    45901 ctggcatgac ttactcagta gctgaaactt ctgtagacaa agttcttact aaagattgta
    45961 aagaaaaagt tgttcaaatg tcttgcttaa ctcctcatgg agttcaatca gtaagtcttg
    46021 atatagaagg attagttgaa agttctacta acttcgctat aatcgaaact aaagaatcta
    46081 ctattgaatt tttaacttct gttagaagtt ctgttatgtc tattagagat gaaatcgctg
    46141 atagaataag acttttggct caagctttag gtgctaatta cgaccttata gctcaatacc
    46201 cagcttggga atttaaaaaa ggctcaaaat tagaaaaaat atgcagtgaa acttatgaga
    46261 aattaactgg taaagttcct actgttatgg ctctacatgc tggtcttgag tgtggattat
    46321 tacttgataa acttcctcat gctgaagcta tctctatagg accagatatg tttgatgttc
    46381 atactccaaa tgaacatgtt agtatccctt ctgtagctaa tgtttgggat tatgttattg
    46441 aaatattaaa atctatgaac caatattaaa tttaagtttt gattctaagt agattttaat
    46501 tttaagtaat ctatataaaa ttaactctgt aaattaatac tactgttagt ataatattta
    46561 ctttcaatat taacttatag aaataaagcc taatgataga taataacatg tatcattagg
    46621 ctttattgta atttaaatat tattaagctt tattctgttt taaaattaat tcttatacta
    46681 ttttaaatct atttcttcct tgtctcttag aatcataaag agcattatct gctatttgat
    46741 acagttcatc atactcttga ccaggctttg ctataactac ccccatacta atagataaac
    46801 tttctccatt tggcaactta atgagatgaa tatgattgta tacatcatta catttattaa
    46861 taagttcttc cttatttatt atatcattga tataaacact aaattcatct ccacccaaac
    46921 gacctactat atcatttttt gcaaaacttt catcaagtac ctttgcaaca tcaataagta
    46981 ctttatctcc taacaagtga ccataagtat cattaatcga cttaaaataa tcaatatcta
    47041 taatcaataa agcaccattt ttttcagttg ataaattcaa caaattttgt gcagttaatt
    47101 ttctaatata tgctgcatta taaatattgg ttaaactatc cctttgagcc ttatctaaca
    47161 atctattctt ttcctctttt tcagaatcaa tattacatat tttcccgatt acatacattg
    47221 gtatttttct atcattataa attatttttg aagtaactct aatccaatga aaagtatcat
    47281 caagcataat agaatacatt tcagttgttc cattttcttg tgattttatt acatcaaaaa
    47341 actttttatt atattctttc cttattttat ctctttcatt ttctatattg ccattttcac
    47401 tttccttatc atttttatcc aaaccataat aagtctcaat acccttttct ggcatagaaa
    47461 ttatcaattt atcttgctta taatcatact caaaaaagta ttcacttgtc atttcataca
    47521 actgagtatg ttttttgagt tctaaagata tcttctcgtt catacttgta cgagtatgca
    47581 aaccaaatgc cataaatcca ataatacaaa ttaataaact ggctacaata aaaatagtct
    47641 caattggatt ctgttgcata atataaagca acgagaagtc ttgtctatat gtagtatttt
    47701 ggtagacaat tgattgcatt ttttctgatg aaatacttat aatagcttta ttaaatatat
    47761 tcaataattc ttgtttatct tttttaatta taccaaaaca aatactcttt gctttataag
    47821 tttgaggaac tagacgcaca tttttatact taggttgatt catatagtac tgcatagaat
    47881 aaccttcgcc ataagtgtaa tctgcatagc ctttattaac agcttctata cactcttcta
    47941 aattatcata ccaaactata ttctcttcaa actgaccata ataattaata cctttggcta
    48001 gagctacacg ttttccatct aaactattct catttaattt attattaatc atcatagtat
    48061 attgagtaga aagaaaaggc cgagacataa ctatattatg ttcacgagag tattcataat
    48121 cataaatcat tcctgctaca atatcaattt cacgcttttt agtcatgttt tctaaatctt
    48181 cttgagattt tacaggcaca agttcaaatt ctaagcctgt tttatcacat atatgattta
    48241 acaaatcaat tgatatacct cttaattcac ctgtatcttt atctatattt tgaaatggtg
    48301 gtttgtttaa aagtactcct acttttaatt tttctgtctc tttaatgtaa tcagtttctt
    48361 catcagataa aaacatttga ttgttctctg gtgtaaagta tttttcatat aaagtagttg
    48421 aaaaatatgg gtctttctgt tcaatattat taattgctga gttaagcttt tgagttaaat
    48481 ttgtactacc tttaggagta atgaaataaa atggttttga tgaaaatttt gctacagtac
    48541 gtacaccttc tagtgggttc atactaacat taagaagtac atctgcttta ccactcttta
    48601 gtgcttctag ttgctcatca tttgttttac aatataccaa ttcaggtgac ataagattca
    48661 ttttacaata ctcatctaac tctttaagtc tagtatcaga attttctatt acagcaatac
    48721 ggattttttt cacatcttgt gaactcatta tgttatcaat atctgtattt tcatataatg
    48781 cttgtagtac tgtatttgct actccataac tatttcctgc ataattatat attttttgca
    48841 ttggctcatt gtataacata cctcccatta tatcaatttc accattttct aacatcttca
    48901 ttaattttgt aatagattca tttatctctc caggaacttg aacaaattca taattccatc
    48961 cagtatactg tgcaatttct agtaaatatt catatgtata cccaaaatag ttaccttgct
    49021 catctatttc agttaaacct ttttgaatag gataagcaac acgaatggta cgattatcat
    49081 tacctatagc gttgatacca aaagaaaata tgcaagaagt gaatatagat gtaattacaa
    49141 taattaataa aagtaatttt tttaaattca ctacacaatc ctccatatag ttaagattta
    49201 cagtataaat tactgcataa acttgtagac atgtaaaaat agattagtat ataaaaggat
    49261 atataaatct acttagaaat ttaaatatcc ttaatatata caatatattt aaattataac
    49321 tcatttatat gtctattaca acaaatttac cctgctaaat acctactcta tatcccataa
    49381 ttttacaagt tgtagtgaag tttaagttct atctcacctc aaatttagca gtgtttctat
    49441 ttacagttct tttatatttc acatctggat aacccaatat aaatgaagtg ataacagctt
    49501 cgttctcgct cattcctaac agttctttta tctttggctc tacatttgat gccataacaa
    49561 aaaatccatt ataacaaatt cctaatcctt gagaatatgc cataagttcc atattagatg
    49621 cagccagtcc tccatctaca taagcacttc cacctagaga catatctcct acaactacca
    49681 taagtagtgg ggcatcataa aataaagaat catttccgtt ctctttatat cttttgtaca
    49741 ttttcttaaa tgtattttta tagatagaac gtacagggtc attgtcatca gtatctaatg
    49801 ccaaattata aagaccttgt attgccagtt ctttcacttc ttttagcttt tcttttacta
    49861 atatgtatct tataggctgt ctattaccac ctgttggtgt gtatctacca gcttccacta
    49921 tgttttttat tttttcttct tctacctcta tatctttata ttgtcttata cttcttctaa
    49981 atttaataaa attcaataac ttttcgggtt ctaattcaaa actttcacga ttgtattcta
    50041 ttacttcatt tttatcaaat ccttgcattt ctattgcatc tgttgggcaa attgccatac
    50101 agtgaccaca gttaatacag agaatatttt taacctttgc tttatcatcc ttaactttta
    50161 ttgcatttac aagacagtct gattcacaca tcccacagcc tacacatttt tctaaattta
    50221 tgtctatcat tccaaattat tcctcctttt aaaaaattta ctttagtata aaacaaatag
    50281 ttgtctaagt caatttttat ttttttaaca atatactagc cttaactaca ctttaatgga
    50341 gaaaaatata cataaattgg aacttaaatt agaacttatt caaagtaaaa gataaaagaa
    50401 gtagatgtct aaaagtgaac tttttagtta attagatatt ctttattgta tgaaatcaag
    50461 tggatattta tcatttcaac aataaaaaag gcttacctct attttggata tgtccctgtc
    50521 aagtagacag actaaaaaaa ggctatcttt atgcactgag atagcccctt ttcccaacta
    50581 ctattgttta tcttaatatt agtcaataaa tttaatttgc tataacctgt aagttacatt
    50641 ttaatttgat gtaactttaa attacatttt taagtaatat ctttaaatta tattcttaaa
    50701 atatatcatt aaactacatt ttaattaatc taaaaactat atcttaatta atctaagaaa
    50761 cttacattat aatcttttag tttcccatta tcatatacaa tctttgcact aaagatattt
    50821 ccagtcttag cccaatttaa gaacagcctg tcaccttccc acatatttaa atcgaatatt
    50881 ttatcattat caacccaaat taaatttcct tctttacaat catttaaatc tttttcagat
    50941 aattcacctt caaactcatc tgagaaatac aaaaacatat accaatcttc ttcatcttta
    51001 aattttggaa aagttatcaa ccctttatat gacaatgatt taagagtaag accagtctct
    51061 tcttttactt ctctaattac acattcttct ggagtctctc cttgctcaaa ctttcctcca
    51121 actcctacat acttaccttc atgtatatca tctttctttt tatttctgta caacatcaaa
    51181 gtcttgtcat ctttttctat ataacaaata gttgtaagtt tcatgtatct cgccttgctt
    51241 tctctttata ttctcaacta attattttca gcactttcaa gcacatcact tatattcttc
    51301 attgcattaa gagaaagctc tgcaaattca tcaaatggaa tccctatatc ttctatactt
    51361 ctcattgcat ctctatttac atttgaagca aatctcgttt ctttcattct tttaataaca
    51421 gacttagtct ttacactggt aagtttttta tctggataaa ctaaagctat agcagttata
    51481 aacccagtta tagggtctgc tgcatatatt gctttttcca ttaggctagt tcttgtaact
    51541 cccattccat catgatgacc aagtattgct ctatacattt cttcgtcacc gaagttttct
    51601 tttttaagca gttctactgt tgttgtagca tgtccctcac atatttctcc tggctttctt
    51661 ccaactatat ctgaatccaa atcatgtaaa agacctgcca ctgcccactc ttcttctttt
    51721 tctggctcta gtcttttggc taattctctc ataacagctt caacagcata acaatgtttt
    51781 ctcattctat cagtttctaa atacttaaat aaaatttcta aagcttcttc tctcattttc
    51841 accaatcccc ctgttttttc acatatatga tatttttata tattaataca cataaattca
    51901 agtttttata tatattcatg tatatttttc atgttatatc tattctacac ctttatagta
    51961 gtgtcctgca actgttccaa tttcatctaa tttcttatac aagtctgtag ccctttttcc
    52021 aagtttataa ttattactca atacctcaat gtgagcttca ataacctttt taaccatctc
    52081 actatcttca actgtaaaat ctatcctaaa tacatctatt ccaatttcct ctaactcata
    52141 tagattgtcc aacatacaag taaccttcga attgtaaatt gtacttctac agaatatatc
    52201 ttgtgttatc ctataacttg catccgtaaa gtctcttaaa gcataactac tttccttaca
    52261 tttagcaact ctcttgtcct ttttacaatc cctaacaact actcccatag gacagtattc
    52321 tgtaaccatc ataggagcat atccatatat tactgcttca atttctacat ctgtatattt
    52381 taaagtttgt ttaatttcat ttaagtttag ttcttgtgac aaacaaactg tacttgctcc
    52441 ctcttctttg aagtggttta tactttcact attgaatgca tttaagtagc aatctatatt
    52501 taagtctttt ttcttaaagt attccataga gccccagtta ccaatttgaa ccttattaat
    52561 tccttttata ttatccaata tctcaaattg cttgtactct ttatttctaa caatccttgg
    52621 tgctgaatag attaattttt tgtcagtgac tgctgtatta gagtggttct tattcaaagt
    52681 atctatttta cttgtatttg acaaagtata tatgttattt gtatttgaac ccattagttc
    52741 tttaggaata cctaaagatg aagaagtata taaatccata gaaatacttg aatctataga
    52801 aatacttgag accatttgta aagcttttga taatgtattt atatcttcat aatagattaa
    52861 atctacatta taatttaata ctgcttctaa ttgctccaag ttcttaactt taactctaat
    52921 cttaggaact gtatcaagtt ttttctgttt atttactcta tttggctggt attttaatct
    52981 attcttctta aattttctat cttttatctt tattctttct tcatctaaaa gctcaataca
    53041 atcccttctc atctgattta acacacttat aggcatactt acaccttcat ccaggtcaat
    53101 ttctatctct tttaacttat atggtgtatt tccaagcttt tgaatttgtg tttctatttt
    53161 ttctttactt aaagccacct ttatagcttt ttcaactatt ttgtctcctt ctaccaccga
    53221 tttatttcca caaaaatcac ttataaaaag ctctggcttt ttacctagtt taatactcac
    53281 ttttgcacgt atattagttt ttatgaactc tttatcttgc tgaaatgtag cttgtactct
    53341 atccatcaat tcgctgtcag aagtcttata tataagctga tttctctttg cttctccaac
    53401 aaaatctaat tctatcaaat cccctttatg tgctattgta gttatctctc catcctttat
    53461 tattcttcca atagtacctc cacctaagtt aattccatca cctttcttaa gcgtattttc
    53521 tagcattact ctaagtcttt tgttctttct attataatct aaaacttttc caatatatag
    53581 cccttgatta ttaggtctat tagagttcat tatatcctta ccaacttcac ctaatatata
    53641 tccttttgta aactttctat taaatatggt atacaaatta tttattattt cctcagaaat
    53701 acttattttt ttcttatcta caaattcatt aatcgcttct ctatatcctg aaaccacagt
    53761 agctacatac tctggctttt tcattctacc ttcaattttt aaagataata cacctgcttc
    53821 aattatttta tccaaatctt ctatagtact taaatctcta ggactcaaca aataatctcc
    53881 attacttttt actacttctc ctgtatttat atctatcaac tcatactttt gtctacatgg
    53941 ctgtgcacac ctacctctat ttcccgacct attgcctatc atactactca taaggcattg
    54001 acccgagtaa catacacata aagctccatg tacaaaaact tctatatcta cagttgtatt
    54061 ttcacaaatc cattttatct catcaacatt taattctctt gctaaaacaa ctctatcaaa
    54121 ccctacactc tgaaggtatt ttacatcttc taaggaatgt gctaccattt gagtgcttgc
    54181 atgtagttca aagtcaggta atagtttttt tataagtctt gccattccta tatcctgtac
    54241 tattaaagca tcaatatcta tatcgtataa aaattttaca tactctacaa aatcttctat
    54301 ttcatactgt tttatcaatg tatttacagt aacaaagact tgcactcccc ttatatgaga
    54361 gtatttaaca gcctcaatca gctcatttct atcaaagtta tttgcagatg ctctagcact
    54421 aaagtcttta ccacctaaat acacagcatt tgctccgttt tgaactgctg cttttaatga
    54481 atcaaaagag cctactggtg caagtaattc tatttttttc atatttttct cctctattta
    54541 tacttgttag gttaatataa ttatatctat aacttaaata tttgtattaa atttatatat
    54601 ttcttactat attttaaatt cttcaatcaa tttatatatt agttatttat atttggttca
    54661 tttgagttaa catatacatt atttactata tttagtatat ttctattaac ttatatattg
    54721 attactgtat tttaaattct tcaattaatt tatatactgc ttactaaatt taaaacattt
    54781 taattaattt gtacactgtt tactatactt gaaatacttc tattaatcta tatattgcct
    54841 actatactta agacatttta attaatttat acactgttta ctatacttga aatacttcta
    54901 ttaatctata tattgcctac tatacttaag acattttaat taatttatac actgcttact
    54961 atacttaaaa tacttctatt aacttataca ttgcttacta tattttaaat tctttaatta
    55021 atctatacaa tatttgctat atataaagta tatcaaataa cagcttcatt tttttgtaaa
    55081 tttacagaaa gttaattttc attaaatgaa tcatctttat tgataaacat aattccaaag
    55141 accactgcca aaataaaaaa acatattagt attgattttt ccacgaaaat ttttgtaaaa
    55201 aaagttccta aaatagctcc agatataaag aatataataa tagcataata ctgtaaactc
    55261 ttttttatca taaaaatatt cttagtatgt atataatgaa ataaagtttc tgtagcactt
    55321 cttaaatttc cagtacacat tgtcgttgca aaactattcc catttacttt tctaaaactc
    55381 tctacttgca ttgaacaaat aaaagaaata gcaacattta caaacatatt aaatcttcct
    55441 ctaggaataa atgaaacaat aagtattgta atcatttcaa ttataatgat aacctgacgc
    55501 caatgaatct ttgtcttatt atttgtaaaa tactttctta tcttctcggc tataaataca
    55561 cctaacataa atgccaaaat aggaatcaaa taataaaatg attcctttac attaccttct
    55621 gctatattaa gcccaaataa aactatattt ccagtttgtg cattagcaaa gactttacct
    55681 cttaccacat aagtataaat atccaaaaat cctcctgtta ttgccaacaa agaacataaa
    55741 aagaatgatt cagacatctg actcttgctt gtcatatata aacctctcgc tttctaatga
    55801 tagatacaaa atcaaaatca ttttagattc tatttttcat tgatataaaa aaattattta
    55861 tatttaaata ttgtctatat ttatacatta tttgtattta tatagcattc atatttattt
    55921 atatttaaac a
//
LOCUS       DAYATN010000010        55880 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.010, whole genome shotgun sequence.
ACCESSION   DAYATN010000010 DAYATN010000000
VERSION     DAYATN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.010"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            586..1236
                     /locus_tag="ACGI8A_000611"
     CDS             586..1236
                     /locus_tag="ACGI8A_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFH0178623.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1450..2121
                     /locus_tag="ACGI8A_000612"
     CDS             1450..2121
                     /locus_tag="ACGI8A_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0178624.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            2118..3248
                     /locus_tag="ACGI8A_000613"
     CDS             2118..3248
                     /locus_tag="ACGI8A_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0178625.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKLEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3549..3671
                     /locus_tag="ACGI8A_000614"
     CDS             3549..3671
                     /locus_tag="ACGI8A_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178626.1"
                     /translation="MKWYTWLTIILIILLSIPLKIKFIKWWDRRQKNDKGGYDR"
     gene            3668..4513
                     /locus_tag="ACGI8A_000615"
     CDS             3668..4513
                     /locus_tag="ACGI8A_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178627.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4513..5541
                     /locus_tag="ACGI8A_000616"
     CDS             4513..5541
                     /locus_tag="ACGI8A_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0178628.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDDYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5544..6557
                     /locus_tag="ACGI8A_000617"
     CDS             5544..6557
                     /locus_tag="ACGI8A_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0178629.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLYYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLF
                     FSR"
     gene            complement(7074..8324)
                     /gene="cme"
                     /locus_tag="ACGI8A_000618"
     CDS             complement(7074..8324)
                     /gene="cme"
                     /locus_tag="ACGI8A_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HFH0178630.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8410..9156)
                     /locus_tag="ACGI8A_000619"
     CDS             complement(8410..9156)
                     /locus_tag="ACGI8A_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0178631.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9271..10293)
                     /locus_tag="ACGI8A_000620"
     CDS             complement(9271..10293)
                     /locus_tag="ACGI8A_000620"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFH0178632.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTYSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10293..12713)
                     /locus_tag="ACGI8A_000621"
     CDS             complement(10293..12713)
                     /locus_tag="ACGI8A_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFH0178633.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSYNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12799..13719)
                     /locus_tag="ACGI8A_000622"
     CDS             complement(12799..13719)
                     /locus_tag="ACGI8A_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0178634.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            14349..15275
                     /locus_tag="ACGI8A_000623"
     CDS             14349..15275
                     /locus_tag="ACGI8A_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HFH0178635.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            complement(15464..16084)
                     /locus_tag="ACGI8A_000624"
     CDS             complement(15464..16084)
                     /locus_tag="ACGI8A_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0178636.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(16056..17639)
                     /locus_tag="ACGI8A_000625"
     CDS             complement(16056..17639)
                     /locus_tag="ACGI8A_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0178637.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNISDNGVANTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISVSDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            complement(17783..19810)
                     /locus_tag="ACGI8A_000626"
     CDS             complement(17783..19810)
                     /locus_tag="ACGI8A_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0178638.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTRILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKK"
     gene            complement(19823..20572)
                     /locus_tag="ACGI8A_000627"
     CDS             complement(19823..20572)
                     /locus_tag="ACGI8A_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178639.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            complement(20871..22523)
                     /locus_tag="ACGI8A_000628"
     CDS             complement(20871..22523)
                     /locus_tag="ACGI8A_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HFH0178640.1"
                     /translation="MNKKNLAILISGIMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVTVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            23111..24232
                     /locus_tag="ACGI8A_000629"
     CDS             23111..24232
                     /locus_tag="ACGI8A_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFH0178641.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            24268..24681
                     /locus_tag="ACGI8A_000630"
     CDS             24268..24681
                     /locus_tag="ACGI8A_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0178642.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            24674..25213
                     /locus_tag="ACGI8A_000631"
     CDS             24674..25213
                     /locus_tag="ACGI8A_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0178643.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(25413..25898)
                     /locus_tag="ACGI8A_000632"
     CDS             complement(25413..25898)
                     /locus_tag="ACGI8A_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178644.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            26803..27180
                     /locus_tag="ACGI8A_000633"
     CDS             26803..27180
                     /locus_tag="ACGI8A_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFH0178645.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            27576..29381
                     /locus_tag="ACGI8A_000634"
     CDS             27576..29381
                     /locus_tag="ACGI8A_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0178646.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            29458..29745
                     /locus_tag="ACGI8A_000635"
     CDS             29458..29745
                     /locus_tag="ACGI8A_000635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178647.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSM"
     gene            30620..31834
                     /gene="dpaL"
                     /locus_tag="ACGI8A_000636"
     CDS             30620..31834
                     /gene="dpaL"
                     /locus_tag="ACGI8A_000636"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFH0178648.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            32164..33351
                     /locus_tag="ACGI8A_000637"
     CDS             32164..33351
                     /locus_tag="ACGI8A_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFH0178649.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            33367..34950
                     /locus_tag="ACGI8A_000638"
     CDS             33367..34950
                     /locus_tag="ACGI8A_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0178650.1"
                     /translation="MRILIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKNLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            34978..36306
                     /gene="ssnA"
                     /locus_tag="ACGI8A_000639"
     CDS             34978..36306
                     /gene="ssnA"
                     /locus_tag="ACGI8A_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFH0178651.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            36505..37857
                     /locus_tag="ACGI8A_000640"
     CDS             36505..37857
                     /locus_tag="ACGI8A_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFH0178652.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            38049..39158
                     /locus_tag="ACGI8A_000641"
     CDS             38049..39158
                     /locus_tag="ACGI8A_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0178653.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVVPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            39441..40832
                     /gene="hydA"
                     /locus_tag="ACGI8A_000642"
     CDS             39441..40832
                     /gene="hydA"
                     /locus_tag="ACGI8A_000642"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HFH0178654.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            40826..43387
                     /gene="xdh"
                     /locus_tag="ACGI8A_000643"
     CDS             40826..43387
                     /gene="xdh"
                     /locus_tag="ACGI8A_000643"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HFH0178655.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            43539..44885
                     /gene="rpoN"
                     /locus_tag="ACGI8A_000644"
     CDS             43539..44885
                     /gene="rpoN"
                     /locus_tag="ACGI8A_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFH0178656.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            45667..46689
                     /locus_tag="ACGI8A_000645"
     CDS             45667..46689
                     /locus_tag="ACGI8A_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFH0178657.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            46749..47756
                     /gene="gap"
                     /locus_tag="ACGI8A_000646"
     CDS             46749..47756
                     /gene="gap"
                     /locus_tag="ACGI8A_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFH0178658.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            47978..49180
                     /gene="pgk"
                     /locus_tag="ACGI8A_000647"
     CDS             47978..49180
                     /gene="pgk"
                     /locus_tag="ACGI8A_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFH0178659.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            49206..49949
                     /gene="tpiA"
                     /locus_tag="ACGI8A_000648"
     CDS             49206..49949
                     /gene="tpiA"
                     /locus_tag="ACGI8A_000648"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFH0178660.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            49961..51490
                     /gene="gpmI"
                     /locus_tag="ACGI8A_000649"
     CDS             49961..51490
                     /gene="gpmI"
                     /locus_tag="ACGI8A_000649"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFH0178661.1"
                     /translation="MKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTLI
                     NASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMDN
                     AKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALEY
                     AKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAIQ
                     NSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDGF
                     KREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAHV
                     TFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVLN
                     FANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPET
                     GKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTILDMMKLEKPEEMTGHSLIS
                     K"
     gene            52078..53370
                     /gene="eno"
                     /locus_tag="ACGI8A_000650"
     CDS             52078..53370
                     /gene="eno"
                     /locus_tag="ACGI8A_000650"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFH0178662.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            53963..54187
                     /gene="secG"
                     /locus_tag="ACGI8A_000651"
     CDS             53963..54187
                     /gene="secG"
                     /locus_tag="ACGI8A_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFH0178663.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(54571..55509)
                     /locus_tag="ACGI8A_000652"
     CDS             complement(54571..55509)
                     /locus_tag="ACGI8A_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFH0178664.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(55497..>55880)
                     /locus_tag="ACGI8A_000653"
     CDS             complement(55497..>55880)
                     /locus_tag="ACGI8A_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0178665.1"
                     /translation="EHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVKINLNI
                     KGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFGNMKSI
                     YLKKTNGNLIELSNHRITESKPWKI"
BASE COUNT        21280 a         6712 c         9611 g        18277 t
ORIGIN      
        1 ttgagtctta tattaaatta aggactataa tttgaaaata agctataaaa actcaatatt
       61 gttttgttca aagataaaaa aagaatactt taggtgattt ttattaaaaa ttaagagtga
      121 taagattata tactttataa atgtaaatat gacggataaa aatgataaat ataataatat
      181 atatatttac attaggagtt ttctatacta taattattta aaactaaata taaatgttgg
      241 aattatttag gacttgaaat tgtctaaaaa taaatataag tgatgaagag gtgatgtgat
      301 gagattagat atggatagta tgttatatgt actaatttat atgacattat tggtatctat
      361 cattggaagt atattttatt tttgtaaaga cctcttaaat cataaaatta agtatgtttt
      421 aataagagac atatttgtaa atagaattac atattctgaa gaacggttgc ctaaacagta
      481 tatagttttt cagaaatatg atatttggag gtattgtagt ttgttttaat ttgaaatgaa
      541 tctattataa attctaaagt ttaaaaataa aaaggagaga ataaaatgga tttcatttca
      601 agtatattgc aaggattatt aagtatatta tttaatttta caggtgattt tggaatttct
      661 attgtactta taactttaat agttaaatta gtacttatgc cactatcatt aaaacaaaga
      721 ttttctgtaa aaaaacagca agaattagct gaaaaaatgg aacatataaa agaaaagtat
      781 aagaatgata ctaaagaatt ggaaaagcaa atacaagtaa gttctgtaga aagtatgaag
      841 agtatgctgg gatgtcttat agttttggtt caaatgccag tagtatatgc tttatatcat
      901 gtatgtttag atatgacaaa ggaatttaca acaattataa ttccatggat tgctaattta
      961 ggtacatttg ataatttgtt tatattacct tgtatatata cattaattat gttagcttca
     1021 aatttagtga attatatacc atatttaaaa ataaattctc aagtaaagtt taacaaacaa
     1081 atggctgtgg tgactaccat aacaagtctt atactaactg ttagaactcc agtagccata
     1141 ggtctgtact ttataacaag tgcaatttat tcattaattg aagatatttg ttttagaata
     1201 tattttaatc gaaaaaatag aatagtgaca aaataatata attattatta gaaatatttt
     1261 agcctgctaa catagaaaaa gttagtaggc tatttttatc tatgatatca attccattaa
     1321 gaatagtcta aaaaatacta tcaaaatgct ataaaaaata ctatcaaaat actataaaaa
     1381 aatagttgaa aaataaataa attaatataa actaaaataa agcctacaaa aaagaggtga
     1441 taaatagaaa tggctaaaat actaattgta gaagatgaaa aaaggatgca aagtattatt
     1501 gtagaatata tgcaaaaggg aggatatact tgtataactg ctgatgatgg agtcgaggca
     1561 cttacaatat taaagagcaa caatatagat ttaatgatat tggatattat gatgccgtat
     1621 ttagatggat tttcagtgtg tagagtttca cgtgaaatga ctaatatacc tattattata
     1681 ttgacagcta aaggtgaaga ggaggacaaa ttaaagggtt atgaatatgg cgcagatgat
     1741 tatataacaa aaccatttag ccccaaagta cttttagcaa aagttaatgc cttgttacgt
     1801 agatacacta ctgatattcc caaaaattca ttgagtttag gaaagatttt tattatggta
     1861 gcatcaaggc aagtgtatgt agaagataaa ttgattgatt taacatataa agaatttgaa
     1921 ttattgcgtt tatttatgga aaaccctaat caagtttttt ccagagagaa acttttaaat
     1981 tgtatatggg gatatgattt tgaaggaaac acacgaactg tagatacaca tataaaaacc
     2041 ttacgcaaaa agcttggtag tgaaggacac catatagtta cattaattcg ttcaggatat
     2101 aagtttgagg ttaaggaatg aatagtttaa gaaagattat agaaaaactg aaaaatgtag
     2161 atatttcaaa ttggattaaa caaaattttg gactatattc agttagaaaa aaagtgtttt
     2221 tactttctaa attatcagga gcgttaatta tattatttta tctggttgtt gaagaatttt
     2281 cgataaactc taaattagaa ttttggattt ggtttataat actggttata tttattatga
     2341 gtattgattt cttattggga aaatttattt cagaaccaat cacatctata aataaatctg
     2401 caaaaagtat gtcccaactt gatttttcta atccatgtac tgtaaatact aatgatgaat
     2461 ttggagaact ttctaggagc ctgaatacaa tgtctacaaa tctacaacag gcattatcag
     2521 acctagaaag tgcaaatatt caacttgaaa aggatgtaaa taaggaaaga atgctattgg
     2581 aacaaagaaa ggagttggta gatacaattt cacatgaaat gaagactcca cttggcatta
     2641 tacgtgcgta tacagagggg cttatggatg aagttgatga agaaaaaaag aaaaattata
     2701 tgaatgttat aatagaagaa actgatagaa tgaacaatat gattgtatca ttattagact
     2761 tatctgcatt ggaagcagga gtttctaaac ttaacccaga gcgttttgat tttatagagt
     2821 ttgttgaaac tgtggcaggt cgtcttttaa ttgatatacc agatgccaat ttttatttta
     2881 catatgattt gccagagtat gaagtttttg tagtggcaga taaaatgaga atggaacaag
     2941 tagtagagaa tctagttatt aatgcaaaga agcatgttgt atataatggt aatttagatt
     3001 tatcgattac atgtgaaaat ggcttgcttt tatttgaaat ttataatgat ggtaggttta
     3061 tagagcaaga tgagatttca aagatttggt ctaagtttta tcgtagtgtt caatctcagc
     3121 gtacaggagg ttcaggtcta gggcttgcaa ttgtttcaca aatacttaca atgcaagggt
     3181 tagaatatgg agtgaataat cgtgatagag gagtagagtt ttattttatg attccaattg
     3241 atgaataatt tagtattaat ttataaatta taagtatctg atgtaaaata tttttctttg
     3301 tatatgtaag catttaaaga gaataaaata aaaaaactta atgaaaaaag cttaatgaaa
     3361 aagtctaata aaaagcttaa tgaaaagtct tgtttttctg atgaaaacaa gattttttat
     3421 taagcttttt attatttcac ttcgatttca caagagagac actctgattt caccaaatag
     3481 aaataaaata ggttaaataa attaaaaaat agaagcgtaa ttaaataagg ttacaagggg
     3541 gagtacttat gaaatggtat acatggttga ctattatatt gataatattg ctttctattc
     3601 cgcttaaaat taaatttata aaatggtggg atagacgaca aaaaaatgat aaaggaggtt
     3661 atgatagatg ataaaagtag gagagttaaa attttcttat ggaaaagata aacagatatt
     3721 gcatggactt aattttgaag taaaagaagg agaaatcttt ggattccttg gacctaatgg
     3781 ttcaggaaaa tccacaacac agaaaatctt aaatggtgtt ttaaaaggtt acaatggtca
     3841 cgtatccctt tttggtaagg aagttaaagc atacacagaa tctttatatc aaaagattgg
     3901 tgttttattt gaatttcctt atttatatac caatttaagt gcaattgata atttagaata
     3961 tttttcgtct ttttatccta aaaatcaaag gagagatata tatgaactct tagatttgtt
     4021 agagtttaag aaagagttta taaataagcc tgtatcatcc tactcaaaag gtatgaagca
     4081 acgtattagt atggcaagag cattggtaag taatccaaag ttgttatttt tagatgagcc
     4141 tactagtgga cttgacccat ctggagctgt actatttcgt aagattattg aagaagaacg
     4201 aaaaaaagga acaactatct ttttgactac tcataatatg ctagatgcag atttaatgtg
     4261 taacaatgtg gcttttattg cagatggaaa gattatggta atagataaac ccaaaaattt
     4321 aaaaatgaaa aatagcaata ataaagttga agtggaattt gtatataatg gaaatcgaga
     4381 tattgaaatt gtagatatac aagagttaga atctggaata acttttaaat atgatgaaat
     4441 cttaagtgta cattcaaaag aacctacact agaagaagtt tttattaaat acacaggaag
     4501 gatgttagtg taatgtactc taaatttttt accttagtaa aaaaagattt ttggatgatg
     4561 atttatggta aattttttgt gctgacactt ggctctctca tactttattc gtgttatatt
     4621 aattttggtt atgtaaagtt tatggatgaa gatagttatc acaataatgt atatctatat
     4681 aatccagaga atataagcat gagtgcatct ccattaatta atcaagtttc ttctataggt
     4741 gaattaaaag caaaacttga tgaagataca agtgcagttt gtattgatat gagttccagt
     4801 aagccgaaag taattttaca tgaaagtatt aaaggagttg acgattatag agctgactat
     4861 gcattgtcat tattatcatc agaaaaagac cacaatagca aaatatcaga caacaatgca
     4921 ataattcaaa ttaatgataa ggatacatct gagataaaac aaagaaaaga catgacttgt
     4981 gagattttat tctttgaaat tgtagctatt ggatttttag ggattgcctc tttattattt
     5041 aaagaaaagc agatgggagt aatgcgtatt actggtattt tacctgtaaa aagaagtctt
     5101 tttatattat caaagatatt tgcttttttg atatgtgacc tatgttttac agttctttta
     5161 gtagtattta atattggatt ttttgaagga ataaaaatac ttccacaagt attagttcaa
     5221 gctagtattt tatcaattat tatgtctttg attggaatag gatgctcaat gttattgaga
     5281 gattttaagc aattttctct tatatatctt gtggttgctg tattcataac tacacctata
     5341 tttctatctg caaatacatc ttttgaaata gattggatta aatatcatcc cttctatcat
     5401 gtttatagtc agcttaaaaa tgcgtttttc atgaattttt caaaagaatt agtttatttt
     5461 gtagtgtgtt ctttgacaat aatagttcta tttttacttg taaaatatat ttttaataaa
     5521 gagttggtta gggagggata atcttgatag gattacgtta ccagttaaag agtataaaaa
     5581 aagataaaat gtgtataatt tccttctttt taccagttgt tatggcgatt ttaataaatt
     5641 ttatgggaac aattgatata tcttcattag gagagatacg ttttggtata gtgaaggata
     5701 cacttcctgt acaaacagag cagtggttga agaagtatgg tatggtaagt gtatatgaca
     5761 caaaaatgca tttaatacgt gaaattaagg atattaaaac agacacaatt ggagttgtta
     5821 gagatggtaa agggataaaa actgtgcttt atggaaatga gattaatgct ataaaaaatg
     5881 ttggaaagac attgccagag atgtttagac taaagggtga tttaaaagat gttgaggtta
     5941 caatattgcc taaagatagt attttaaaag agtttcaaaa tgtttttatt gccatgattt
     6001 taattactgc tatgtttatg ggatgcacct ttaatgcaat gaatattata tctgaaaaag
     6061 aagatggaat atcatttata aatgaagttt tgccacagag tagaagtcag tatattatac
     6121 aaaaagtatt tttgggtctt ttatgtgggt gtttatcttc aatgataact gtatttatat
     6181 gtttggatat ttcaatatta gatatgataa tgatgttgtt actcgttgta ttttcagcat
     6241 ttatttcatc acttataggt ttatttattg gaaagttgtc agaaggtctt atggtaggaa
     6301 ttgtttatat taaaatagta atgattttat ttataggtac tcctttgtta atatatcttc
     6361 taggggttaa taccagtggt atagggaatt tatactatat aatgccttca gttgctacat
     6421 ttaatggtat tatggagatt attaatggta atgtcattat tatcaaagat attgtaattt
     6481 tggctgtaca ttgtgtagta tggtttatga tatatattat tttagataaa aaaagaaagt
     6541 tattttttag tagataactc agtataatta taattgggac aatttaatag gtgatttaat
     6601 atactttgta tagtgtaatt tatgtagtgc aatttgtata ctttaattta tgtagtgtaa
     6661 tagttagttt tattttataa ttattcaata taatttagta agaagttcag tacaattaga
     6721 gctacttagt ggaaaactta ataggtactt aaactatata aatactttat tttcctccat
     6781 ttgtactata aatttaattt atgatataaa tggaggtatt attatatgat ttgataaatt
     6841 tcatcattat aacctacaag taaaggtatt attatataaa gttaataaag atagtatcaa
     6901 aatattattt gctataaaga gaggggctca aaataaagat aaacctattt ttcaatgttg
     6961 aaatgataaa atatatttga tttcatataa aaaattttct tcataaatta aaaagtccat
     7021 tttaagcccc ctcattttta accagataaa tcgagttaaa ctatttaaat atattactcg
     7081 gattcatttg acttagatat tccttgcttg taaaattgtt ttgaataggg gatagccaaa
     7141 gcaaaaaata aaagtataat ccctgctatg attattaaat tattaataga aattacatct
     7201 gccattggtc cgaaaatcat cataccaaga ggaataaaac cagtatatat aataataggt
     7261 aagctaagaa ctcgacctag catttgtggt gctacatgct cttgcaacat agtcatagct
     7321 gatgcttgaa tgattggaat tgaaaaattc gtaaaaaaca taaatataaa aaattgccaa
     7381 gcttgtgtta caagacctag aataattgaa aaaatagcat aggataatat tgctagaaaa
     7441 aaggtcttat ttctattttt aaaaccacca aaaactccta taatcaatcc acctaaaacc
     7501 atacctaaaa atccaatcat ttcaataata gacaggtata tataaccctt gccaaatgtt
     7561 cgttcaataa gaagtcctat aagaaatcct gatggaacac aaagaagcat gaatgctcca
     7621 taagtaaaaa gcacttttct aatgaactta tctgaaaaac agtactttat ccctgatacc
     7681 atatctgcaa aaaatgagac tttttcagtt tgttttggta tatcatgttt aggaattttg
     7741 atgaatatca ataatacaat agcaataatt gcagtaaata catcaattaa aagaacttga
     7801 tttattttat taccaaatcc aagtattgca gcagctacaa taggaactat aaattgtact
     7861 atagattgta ggctactatt tataccatta aaacgcatta aatgttcttc tggtacaagt
     7921 tgaggaatca ttgcgtttac agcaggtgtt tgtacaccac ttcctagtga acgtataaca
     7981 gatattactg atatggctat taattcactt gttgtgccat ttgatgacat cataaataga
     8041 attagaacta atgtagatat agcaattgtt gaatctgata caattatgag tacctttcga
     8101 ttgtagtagt ctgcccatac accagcaaaa aaagaaatta tcatttgagg tataaaggag
     8161 catacagtta aaactgttac ccatacacct gatgatgtct gaagtgtgac ataccatatc
     8221 attgccattt gtacaagtga tgagccaaat aaggaaatat attgacttgc taaaaaaaga
     8281 atgacttttt tcttccatgt agaattagta tttttgtttt ccatcctatt cctcctttga
     8341 tttgtgtgtt gaatttttct tgctcttaag gataacacac aaatcaaagt ctggagttag
     8401 agtggagggc taggaaaatt gttttttcat aatttgaaga tgagatttca tggtttttgc
     8461 attatgttca gcttctttca atgaagttat cagtttgtca cacactgaga taatttcgtc
     8521 atcatctttt ggagtgtcaa ggacagtttt aatattagta ttaggattag aagataaagc
     8581 atttaacatt cgtaaaatag ctgaaagaga atagtttgca caacgaagtg cgcttataat
     8641 tttaagtcgt tttatatctt catcagtata tacacgataa ccattttgtt ttctttttac
     8701 ttttaacaaa ccattcattt cccaatttct taaagtatct attgtaacgt ttaagtgttc
     8761 tgcagtttct tttctagtta aaaagagttt atttgtttct ggagtatttc catttaaaat
     8821 ctgtgcaaca atagtaagtg cttcctcggc attagatttt tctatttcta attgttgcaa
     8881 gtattgattt acaagactaa tagccttatc aaagtcccta ttcgctgatg ttcttacaat
     8941 ctcaagggca tcttttctta atcccctttg taccacttca attttcaagg ctgtacgagc
     9001 aaatctaaat tgttctatat gaaaatcatt aaagatacga taaccattgg tattacgctt
     9061 aggtttaggt attaagttta gttcttcata taaacgtact gtattaggat gtacaccaat
     9121 taaatctgct acctctgatg tcgtgtaagt gtccattagt attcaactcc ttttagatat
     9181 atagtattag tcataataac accaaacaaa agtctggtgt tataatggag ggtttggtta
     9241 tctaagagta attaatttat gaaagagtaa ttaatctatt atagaagtaa gtatatattt
     9301 ttcaataaac tcccatctat cacgaggttt aaacactatt ttagatggta tggctattac
     9361 taagtcatat tgtggaatac aacaaatagc atttcctcca gctcccattg ctgaatacca
     9421 caaaacacca tcttttttac atagccacca cagataaccg tattcattac tattcatttt
     9481 aactgattct tcaatccacg atttagatat aatacgttta ttattccaaa taccatcatt
     9541 taaatacaga aaaccaaatc gcaacatatc tctagcactc aatgtaagtc cccagccacc
     9601 tatagaataa ccctgtctat cttttgccca acccttaaga ttttttccaa agaaatcttc
     9661 aaaaccaaaa tctttcatat cataatctgg tataactttc ataccaatag gtttaaataa
     9721 gtattcatta gcaaattcac gagcagattt tccagtagtt tttgttataa tagctgaaag
     9781 taggtgcgtt ccagctgaag aatatttgaa agtaccaatt tcgccttttt gaccaagttg
     9841 attcaaggta tattttatcc aatctttttg tttgcaaagt ctttctaaag actcagtcat
     9901 atttttaaaa gggtatggtg cagtcattgt aagtaggtga cgtatagtta ctgctttttt
     9961 acatatttca gaaggactat aagtatattc agggaaaaaa tcaacaacct tttggtcaac
    10021 atcttttata tatcctttat ctattgcaat acctattaag gctgatataa tactttttgt
    10081 aactgatgca acatgaaaag tatcatcagc attatatcca ttatagtact tttcaaagac
    10141 aacttttcct tttcttgcaa taattattcc atttatatta ctatattgtg agattattaa
    10201 aggttcaagt tgtgagagtt tttctgaact catgccaaca cttgaaggat ttactttttc
    10261 aaatattaaa tctaagcaat tgtttttatt cattaaagat acctccctgt tatagatttt
    10321 ggattttctt ttagagtttt tggagaacct tctgcaatta tataacctcc tttgtcacca
    10381 ccttctggac caagctcaac aatccagtca caaacattta atacatctat atcatgttca
    10441 acaacaatta cagaatttcc attttcaacc aattcatcta ataattgaat caacttagaa
    10501 gtatcgtata gacttagacc agtagttggc tcatccaata cataaaggat attaccattt
    10561 cgttttttac caatttcttt tgcaagtttg attctttggg attcaccacc acttaaagtt
    10621 ggtgtaggtt ggcctaattt tatatacccc atacctattt gttctaatac atttaagtgt
    10681 gaaacaatat ttggaatatc cttgaagaaa tgtacagatt cagaaacact catatttaaa
    10741 atatcatgta tatttttatt tttatattta atagataaag attcatcatt aaatcttttt
    10801 ccctgacatt cactgcatat atggtctata cttagattat ttcccaacca gaccttttca
    10861 tatccactac cactacattt aggacaagcc cctttggagt taaatgagaa atgaccagct
    10921 gtaaatcctc gttctaacga ttctggctga cctgcaaata gtgtacgtat cttatcccat
    10981 ataccaatat aagaagcagg agttgagttc atatttttgc caataggaga ttgagatatc
    11041 tctgaatatc cagaaatatg tttagtacca atcagtctat ctgcaatagt ttcaacaact
    11101 gcataatcgt cttcctcacc tatttcactc tctgaagaat ttatttgatt attgatagta
    11161 tggttattaa attggcttct aagaagagga agtagggtgt ctgatataag tgaactcttg
    11221 ccactacctg attttccaga tattccaacc atagcaccca aaggtaatga tacagttaca
    11281 tttttaagat tatttgtatt tgcattttga atagttatac aaggtatttt atcagtatgt
    11341 gtaatgtttt tattgcttgt tctattaggc attggatatt ttccagatag atactgtcca
    11401 gtgagtgatt tgtcggattg aagtagacct tctaaatctc cttgatatac taattgacca
    11461 ccttcaacac cagcttttgg accaatatca ataatatgtt ctgccatttt aattgtgttt
    11521 ttatcatgct caactactat gaccgtattg cctaaatctt taagtttctt tattgatttt
    11581 aagatgtcta ttttttctga ttcatgcaat ccagctgttg gttcatcaag cacataaatt
    11641 aatgagtcca tctctgaatc gagatgtgaa tttaaaaata gtctttgcat ttccccacca
    11701 ctcaaagttg acatttctcg gtataaggat agatgaccta atcgtgattc tattaaatgt
    11761 tgagttttgg ataaaatatc atttacaaga tttgtaccaa atgtgtttaa attaccttga
    11821 tttattaaga gtttaaggaa atcatcaatt tctaaaatcg tcatttttcc aagttctcca
    11881 atatgttttc cgtttaaaag tactctacgt gcttcttcac caattttaaa accattgcaa
    11941 tctgaacagg cagtcatagt ataaactcca tttaaatctt cgcccctttt gtaacgattg
    12001 tgaaatacct ttgataaaga ggaacttttt ttaccatttt cataatgacc atagagtact
    12061 tcatttctta cttcttcagg tagttgtaag aattgtatgt ctaaataatc attgaatttt
    12121 ttagaaaata ctctcaaata tccaggtgta atctttgctc tatctaagac ttgttttagg
    12181 gtatcatgct tatcaaggac taatttttca atattaatct caaaatatgc accatgtcca
    12241 gagcacttca tacacattcc atctgaagag ttgtatgaga aataacttgg tcttaatcgt
    12301 tcttcctcat atccacaatt attgcaaatt agattatagc ttacaggagt atcacacata
    12361 gtacacatta tttgaccttc cactgaatac aatagtgtaa gcatgttgag aatattggtt
    12421 cttgtgccta ctgttgaacg aggattgctc tggcgaataa tattttgttg aacagctata
    12481 gtgggagcta aaccttgaat attatcaaac ttatcttcac tatcaatacc tgcaagtata
    12541 cctaaagatt gcagatattg tttacgccct tcctcaaata caatgtcaaa cataaggcta
    12601 gacttacctg agccactgac acctgttgca acaacaagct tatctttggg gatagaaaca
    12661 tctatatttt taagattgtg tatctttgct ccattaattt ttatttcttt cattggttca
    12721 cctcatttta tcaattttat caaaccgtaa aatagggata tattaatcct taatacaaga
    12781 atagattagg tgaggttatt aaaaaggttc tataggtaaa tatatatcga ccaagtgtct
    12841 attttgaggg tgattgtgtg ggttgttaag atatacttca aaagggaagg aggcacgtag
    12901 cttataatta ccatttgata gccattcatt atacataaaa tcccacgcat cactatattc
    12961 agattttgaa atatcaaaat gtcccacaag atattttcca gaaggtatta tcatattgcc
    13021 aagattgtca ttctctttga caacaatgtt atctggaatg gtcatgcaaa tactagtcct
    13081 taattgatgc tcctgtgtaa attgtggatt atcatgataa atggctaata tttgtgtgtt
    13141 ttcaagtcca agtagattct gctcacttgc atatgtgaat aaccgttcta aaagttttgg
    13201 gaaagtactg gttagattac catatgaacc agtataccta gtgtaaatag tattcatatt
    13261 atcaagttct agaatttcaa tttcaccttt aacctctcta gtattcttac atttaggttt
    13321 tgatatgctt ctattgtact gagaaatttt atatggttct ttgcaattct tgctataatt
    13381 gtttctatat tttatagggc ttattttata gtaattttta aatgctcttg agaaaactgc
    13441 tgaatcagta aagccaaagt agtgtgcaat atcagtaaca gtcatgtcag ttctatgaat
    13501 aagaaaagca gttgcatttt ctagttttag cctatttaca tattgtgcca aagtttcttt
    13561 ttctattgct ttgaagattc tatgaaaatg aaattttgaa aaacctgcta tattagaaag
    13621 ttcatcaaga gaaagaggtt catgtaaatg ttcttctata tagtcttgca ctttgcaaat
    13681 acgatttaga tattcttttt tacttttagc atttgtcata tatagccctc cttattgtgt
    13741 aaaatgtgta tcaattaatt tatagcaata tagtaataaa attttatgtt tttgtaattt
    13801 atgataaata agatagcgtt taatttttta taattgataa aagatattta taattaataa
    13861 aagatattta taattaataa aagatataag atgccttata tttgtctttg catataagta
    13921 tagtcttcgt aataattagt ataccatagt gatttatttt taattgtgta tttatataaa
    13981 tacacaactt atgatatgga cgaattatta tgcaaataag ctgtaattaa ataaattgca
    14041 atataaacca gttgaaacat aagttaggga aatattatca aagttattac ttagcataag
    14101 agtatagatg gaataaaata agattaaaaa attacatact aaatttaata aaatttttat
    14161 attaaaaata atattaagtt tattgtttaa ataaaaaagt taaatctaca ctattaactt
    14221 attatactta aaactttaaa atgaaaatat caaagaatac tttttaataa aaataatcat
    14281 ttaataagtt atttaaaaat ctaaaaactt tacaacaaat tatctattta aattggagga
    14341 gtgggaatat gagtaatagt ttttatcaat taaattcagc agtattatat tttaatgaaa
    14401 aaagagatgt aaatagaata aggtttaatc actatgtaat gagaaaagaa aatatacaaa
    14461 gattaaaagg aactctgcca agaggacaat ttatgatgac agtaaggaaa gctgcatctg
    14521 accttgatgt atctatatct acaataagta gacttgtaaa tgaatttata gatttaggta
    14581 tattgagatt aataagtagg ggagtaaagg gaaattgttg ttctgtatat agttatgttt
    14641 gttctgaaag tagtaatgca gaaattgtaa caaagaatgg tgatattttc aaaaatatag
    14701 agaaatacat tgatgcaact attaacaata accaaaaaac aatgaaatat aaacatagaa
    14761 atgagttgtt taagaacagt aataccaaca tatacagccg tataaatcaa gagagtatag
    14821 acaaagatta tgatttgata attcatactg ggtatgaaac agaaaatgag acaaagaaaa
    14881 aagaattatt aaaaaataat ttaaataaaa agaatttaaa taaaaatacc tttgaaaatt
    14941 cggatgaaga tttgactgaa gattctactt acaaaaatcc agaaagcata catgaaatta
    15001 ttataagaaa attaaatagt gaatcaggaa aaaattttaa agctgatttg agtataacta
    15061 aggcacttat agataaaaga ttaaaagaag ggtatagttt agaagatttt tacaaggtta
    15121 tagaggcaaa ggtaagaaga tggagatata cagatatgga aatatatata gaaccaacaa
    15181 ttctgtttgg gtatgaattt gagtcgtatt taaatgagga gagttcttta tctgcttgta
    15241 attatggtga tatgtgttat gaagatatgt attgagatat tagttgcttt ttagttgttt
    15301 aaaatatgtt tatatataaa tataaatagt gtattaaaat ggagttgtct caaaatagag
    15361 agaagcctct tcttaattgt ttaaatgatg aaaatatatt tgatttaata caaaggagag
    15421 tgctttatga gttagaagat ttattttgag acactcactt tttttaatat actggagaaa
    15481 tataacccat tttaatagct tttgtaatag cctcaactga tgatgaaaca tctagcttgt
    15541 catatatgtg ttgtaaatga tttgataatg ttctttcact tatatacaat tcatcagcaa
    15601 ttatttttct ttttttacca ctacagataa gctgtaaaat tcttttctcc atagtagtta
    15661 aatcttcaat aattacttca gtattttcag atggaaaaca agttacccct tgatagactt
    15721 ttaataatga atcaattaaa ctatcagggc taatattctt attgaggaat ccatagatac
    15781 ctattttttt agcctcatgt ttataaactg ggaggtcgta tccagtcaag attattattt
    15841 taatagttga attagtctct aataaattat aagctacttt taagccatca cattcactta
    15901 agcctctaag gtttatatcc attaagacaa tgtctatttt tttatcttga atcatttctg
    15961 aaagattatg gatgtcctgt gtggaataaa actgttcgat ttctttgtat tcttctagtg
    16021 caattgacaa gcttttagca aaaagcatat ggtcatcaac taacaaaata tttataagaa
    16081 ccatctcctt tcatagggat tgtaatctgt acacaaactc catgaggtat attatcagaa
    16141 acacttatcg ttcctcctag attttctact tgttctttaa ttgatataat ccctttgtgt
    16201 tttattgtat caaaagtaat tttagttata tctaaaggcg ttttaaaaga tgtatttgcc
    16261 acaccattat cactaatatt taattttatt atattttttt ctagtgctaa agctatccat
    16321 gcatggctac catttgaatg tttatacaca tttgtcaata attctttaat cagtcgatat
    16381 atcaatatat catatggttc aactaagaat aaagtatcag aacacttaaa tgaaacacaa
    16441 atattttttt gtggaaagga tgaagaaaca ctttcaatta aattctgata attttctttt
    16501 actgttaaat tttttaaaat aacaggatga tagtcttgca tttgcttcct tatacatgta
    16561 tttagattgt taagtgtttc atatataata tcttgaacat ctgggcgata tgacttactc
    16621 atcatgtttt ttattgaaag caggttctgt aatatgtcat catgtaaaaa attagaaaac
    16681 tctgttttta gttgttcttc ttgttgtaat ttttctaatg caaaagtata cttgctattt
    16741 ttaactactt tatttttttc atctctaagg ttaaactcca aaataatgtt acagaggaaa
    16801 atgagcataa ataaaacatt tatagctatt aatattatag aaaaattccc attggcaaat
    16861 agtacgacca agctaattac taaaatactt ataaaaagaa atatagccct ttgttttatg
    16921 ttaaaaactg ttgaaatagg aaaactatca ttttctttaa gtgcaatacc atgaatactc
    16981 ataaaaaata ccagtacagt tagatagatt ccaaaattac caatatgatt tgatattctt
    17041 agtgtaaaaa aataataagt aaggaatcct ataattgtta taaatactga aatcgatata
    17101 ggtttttttt cgctttttat gacaaaaaca acccttttca aatgataaaa aataatatag
    17161 aaaaagctta ttatactaac aacaaactgt attccgtaca tacaagcata cgttctgtta
    17221 gaaataaatg ctcctacaat tgcaccaatg caggtgataa gcaataaaat gtctgttatc
    17281 ttacggaaat tgtatccact accttgaaac aaaaataaaa atataaattt aatagagaca
    17341 tacactataa ctgtgcttaa tattttaaaa ataatagtgc ctattggtgt ggttacaaat
    17401 gataagagta aataccaact gtctagtgct agtaatccac aaaacaatga aataatgtta
    17461 ttttctcgga cattacaaga ggttatatat gtgatgtcta tgagtaataa tgttgaaatt
    17521 attaagggaa taatgctttg aatattcata taagtttcag tgcttgtaga tattcccatg
    17581 aagagaagtg atagaatgta gtttcctaaa aatagtattt ttaaattaaa atttgtcaat
    17641 cttatacacc tccttatgag ttaattatat taattttatg actaaatcaa acttaagaat
    17701 ttatagtttt aaattatatc aaatacttat aaatgaagta taccaaatcg tcacaccttt
    17761 aagcttgacg atttggcaaa tattattttt ttaatacaat tttacaacag gatttaatag
    17821 taagtatgta atatatcaaa tatactacag aagttaagaa aattgcaata atcacaggta
    17881 tgtattgtga aagtgaattt ccaagtaaat tctgcatcaa agcagtctta taaaccaagg
    17941 tagcaaatat acagtccact ataccaagta aaaacggaat attaaaaaat ggcaatactt
    18001 gttttttaat aattttttta atttgtatat tagtataacc cattttctgt aaaattttat
    18061 aatcagatgt tgaatctgaa attgctgata tattgttaaa gtataaaata cttccaacag
    18121 caataaagaa taataccaca agaaatccga ttaacagaaa tgtagaacta ttaattgata
    18181 ataaatcatt tatcctgtga gaatatcctt gtaaataagg gcttccatct aaataatctg
    18241 aaatattact atataaattt tcattatttt taattgattt accattaata cttaaaattc
    18301 ttgtcattgg taatttattt tgtgctattt gctcatatat actatcatta acgataagtg
    18361 ttccaacgct attggcaaaa gaaatagcat tgtttaatga tgtttccctt acagctacag
    18421 gtgtttcttt accattaaca agtaatggat aaatcgttcc attttcacta tatccatcat
    18481 tatttggttg atatttcatt aaaatacatt cattatccct taaaggggta agttgtttta
    18541 gtagctttgt tcgattttgt gcctttaata aagtataata atcagaaaaa gatatacatt
    18601 caaatcctga ttctcttgaa ataacttctt tagaagaact actattggat aaattattct
    18661 tagatgtacc aatttcatat tcaaatggaa ggtcttttga tgaagatttt accttataaa
    18721 tatttgtctg tgtaaaaata atatcatctg tattagacgt atattgactg acaatttgtt
    18781 ttacatcttc tatttgttta tttttttcta ctctaaattc tatctctgaa ggtgcaatac
    18841 gcgaaacaga tgcaataggg tagtataagg acagtgccat aacacttgaa actgtcaaag
    18901 tagtagcaga gagtaatgta agcataatta gagttttaga gtttgaacgt atacgataga
    18961 taaagtttgg aatagctatt atcttcatat ctgtgtagaa agtacgttta ttttttttac
    19021 tattttgcat aatatatgga agaaaggaat tgataaagag aactgtacca attacaatga
    19081 taatcatagt tatcaagccc atttgataaa atccaatact tacccatact gatttagctc
    19141 cacgaagaat atctagtgat aaacaatacc cgcttaaaat cataataaac cccataaagg
    19201 ctggaatttt tctgaacttt aatttttttt ctgcactttt ttcatatctt atcaatttta
    19261 caagtggagt cttataaaaa aatttaccat ttgatacagc taagactaaa acaactaatg
    19321 ctataaagat aattgtttta tttattgcat ttatattaaa aaatggtatc tgtgaattat
    19381 caattgataa attaagtagt gctgtaattc caaagacaat acccttatgt gccaatgctc
    19441 ctaataccaa tccaataata aaagctccac tacaaattaa tacattttca aaggtaagta
    19501 gcgataatat tgtaaactta cgatagccaa gtaaagcata tatacctaat tcttttgtac
    19561 gacgtctcaa gaagaatcga tttgagtatg ccatatagaa gatgacaaat gccatcaaaa
    19621 atatcgaaat aacattacac atagattcaa ctctaccatc agttgaaatt ttttctagca
    19681 ttatcttatt aacagaaaat gaagtaaatg cgaaaaatac agtaataaca aaagagactg
    19741 aaagtagata tagagaatag aatgaaaagt tttttttaag attctttatt gccgtataaa
    19801 aaatgttcat taaattatcc tcctatcttt tagtatccaa ttttgcaatt tcttgaataa
    19861 tcaactcata aaatgtatgt ctattattct gttgtttatt taattcttta atgatatgac
    19921 catctttaaa aatcaaaatt ctatttgcaa aacttgctac ataggcatca tgtgttacca
    19981 ttaaaattgt tgttttcaac tcctcatttg cttcctgtag tgagtggagt agttcagtag
    20041 ctgaattaga atccaaagct cctgttggtt catctgcaaa gagaatacta ggatgtttta
    20101 ctattgctct aactgctgaa gctctctgtc tttgcccacc agataattca tttgggtatt
    20161 tatttaattg agaagctatg ccaaatcgtt cagctagact ctttaccatg ttttctattt
    20221 tatctggatt gagtttctga agtgtaagag gtactgaaat attctcgaaa attgtaagac
    20281 tatctagtaa aaaatactct tgaaagataa agcctaaatt atccttacga aaatcagcag
    20341 cacaagtgtc attaagcgat ttcaaattta tattatttat gaaaatatct ccacttgttg
    20401 gtttatcaat tgtagataat acattaagta gagttgtttt cccagagcca gaagctccca
    20461 ttatacctat aaattcacct tctgatacat taaaggatac cttatctaaa ctcggctgtt
    20521 gccctttatt ataagtttta gttatgttat ttacttttaa tattgtattc attgtatatg
    20581 tctcctttcg tcttaatata gtcaaattat atattaacct tattgtttct tcatgagtaa
    20641 atcacgcaat aattctacac aaattactta tatcacaata tattgtttaa aatctaactt
    20701 tattaggttt gggatgatgg acaagatttt aatttaaaag agtgcatagg tatatataat
    20761 ggcataatgt ggatttaaat taaaaaaaat aaaataaagc cataaatatt gaaattttca
    20821 atatttatgg ctttattgct tgtaataatt aatagaatat atatttttta ttaatcatct
    20881 atttcaaatt ctaatatttt accagtaatt gcattaattg ttatctcata ttcagttttt
    20941 ccttttatta tttcaccttc atattctttg tcatcttcat catacttaaa ttttactatt
    21001 ttcccaccag gcactttatc tagcattatt gatttagctt tttcagcact aataatagaa
    21061 ctgttgtcat tagtattaga gttattctta tcattatcat caaagtcatc atcagaatca
    21121 ttgtccatgt caatatctac aattcttcca tcaaatgcac ttatctctaa ttcatatgtt
    21181 ttcttatttt ttattacttt agcctcatat tcgttatctt cagcatcata tttacaatag
    21241 attaaattac cattaggaac agcatctaat gctatagact tagctttatc caatgatata
    21301 ttttgtgtac ctaaattatt atcatttaag tcatcatctt tttcaatttc tattatttta
    21361 ccagagtagg catcaatttc tatctcatat aggatattat cctttataac tttgcagtca
    21421 tattcattat catcttcatc ataataacaa actaaaatat tgcttcctgg aagagcttgt
    21481 tttaatattt ctttagcctg tattaaagat atttttttag ttttatttct tatgttcaca
    21541 ttttttagtt ttaaaatatc ttcaaatgct ttttgattat atcctcctcc tacttgtgtt
    21601 atttcatcta tatctttatt tttaataaat tctttttgac tatttacaag gctaccatta
    21661 gatatcatta taggagaatt ttctttagca gctagtactc ctattgataa tgaatctact
    21721 aatgcatttt cttggttaat accatcttta gctacaaata cattatttat ttttttatct
    21781 ttataaaatt tatcaataat ttttgcatta gtattgtttc tatctgaacc actgattctt
    21841 tctctgtttg gatatttctt tactgtattg tctgataaaa tcttatttcc tccaactatg
    21901 taagattttt ttatagacct attgtttaag aaatttatac tactatcatt gctgttttca
    21961 tctgatacta atattatagg catattatgt attgctgctg gagaagctat actaactgca
    22021 tcagatagtc cctttagtcc atttacaact gtaacatttt ctaccttttt tatcttatct
    22081 atttctttaa gtacatttgt agacatttca tatatattgt ttcctactat tttatttatt
    22141 tttatgttaa gtgattttat ttcattttct actttagaag aaatgctatt tccactatct
    22201 acaatgtaca cattatctac accaagcctt ttaagctcag ccttagttga attttcaata
    22261 ctgttgttgt ttactaaaag tataggtgca ttctttagct tagcaaatgg atttgcacaa
    22321 agcgcacttt gaatagaatt accattaact ataatggctg tttttgattg aacccatccc
    22381 aatttactta cttctactgc tgtttcatat cttgttttac ctattaatct ttcttgcttt
    22441 gtagtattgt ctgcaaaaat tgctgaagaa ctagaaaaaa acataatacc tgatattaat
    22501 atagctaaat ttttcttatt catattttta cctcacaatc tttaaaaatt tgttatttag
    22561 catacttaat aaaatataat cttaaaatca aattgatgct gagtttgaag ttacaaaatc
    22621 aacaaataag atttttttaa tacaaaattt tcctttttat atttaacatt gtatcacaaa
    22681 tataaaaagg aaaaaacttt ttaaaaaggg tatacactat tcatttttta tcttataaat
    22741 aagtctttac cactgtttta tatattttaa aatagacaca agacctattt ctaaatcttt
    22801 taggtgaaat ttctttttaa acatatctaa aaagtatttg tagtaatatt aatggactga
    22861 atttcttaaa gtatatattt ctttacatgt tggagtttgt gaagtttgag aattggaggg
    22921 tgaaagaaac atgtaatgga ttaatattaa agaactgtat ttttataata tttgtcttaa
    22981 aaataaaaaa atctattgac ttgaagttaa ctccaagtag taaagtataa ttatacaaga
    23041 attatatact tgtaagaata gttaaagtag agagaaaaaa caaacaagtt ttaaaggggg
    23101 aagtaaaaca atggaggcaa aaaaattagg atttggatta atgcgtttac caataaaaga
    23161 tgaaaatgat gttacaacta ttgatatgga acatttaaat aaaatggtag acactttttt
    23221 agaaagggga tttacatatt ttgatacagc ctatgtgtat cacatgggaa aaagtgagat
    23281 agcacttaga gaatctctag taaaaagaca caaaagagag agttttacta tagctacaaa
    23341 acttccatta atggctttaa agaaaaaaga agagcaagaa accattttta atgagcaatt
    23401 agaaaaatgt ggtgtagact attttgacta ctatctttta cacaatatag gcgtgtcaca
    23461 ttatgaagta gctaaaaaat ttgatagctt taagtttatt gaagaaaaga aaaaagaagg
    23521 aaaaataaag aatattggtt tttcttttca tgatagtgca gaattattgg ataaagtctt
    23581 gagtgagcat ccagaagtag acttcgtgca attacagatt aattatttag attgggataa
    23641 tgaaagcata caatctagaa aatgctacga ggtggcagag aagcataata agcctgtaat
    23701 tgttatggag ccagtaaaag gaggtacact tgcaaagata ccagaaaaag cagagaaatt
    23761 attgaatgac tacaatccag atatgtcaat ttcatcttgg gcaatacgtt ttgcagcaag
    23821 taaagataat gttatgatgg tcttaagtgg gatgtcaaat atggaacaat tattagacaa
    23881 tacaggatat atgcaaaatt tcaaaccatt cgtacaagag gaatataata ttatagatga
    23941 agcagtagag attatcaacg aatcaatttt agttccttgt actgcctgtc agtattgtgt
    24001 agaaggctgt ccaaaaaata tcgctattcc aaactatttt gcgttgtaca atgcagaaaa
    24061 acaagctctt aatactggat tttcaacaca gatggtctac tataataatt atactaaaac
    24121 ttatggaaag gcctcagatt gtattgaatg taaacaatgt gaagaaagct gtcctcaaca
    24181 cattaaaatt atagatgctt taaagaatgt agcagaaacc tttgagaaat aattcaatct
    24241 atacaatatc aagaggagaa gatatgtatg acgatttcag aagttagtaa aaaatacgag
    24301 ctttcagcgg atactttacg atattatgaa cgaattggtt taataccacc tgtaaataga
    24361 aataaaagtg gaattaggtc ttttacagaa gaagattgtg agtgggttaa tttcataaag
    24421 tgtatgagag gtgcaggtct ttctattgag acattaattg agtatgtagc tatgtttcaa
    24481 caagggagca gtactatcaa agctagaaaa gaacttttaa tagagcagag aaaccaacta
    24541 gcaaaaagaa tagaagaaat gcaaaaaact ttggaacgat taaattttaa gattgataga
    24601 tatgaagaag gtattattga gaaagaaaaa gtattaaaga gcagtagaaa taagaaaatg
    24661 gaggcagtat cttatgagta aaaatgtatt aatagtatca gctagtccta gaaaaggtgg
    24721 aaattctgat ttattgtgtg accagtttat acaaggggta ttagaatcta aaaatcaagt
    24781 ggagaagata tttttaaaag ataaagatat taactattgt actggatgtg gtgcttgtta
    24841 tgaaaaagaa gcaaattgtt cacagacaga tgacatggga gaaattcttg aaaaaatgat
    24901 tgaggcagat gtgattgttt tagcgactcc agtttatttt tatactatga atgcgcaaat
    24961 gaaaactatg attgatagaa cttgctctag atatactgaa atttgtgata aagatttcta
    25021 ttttatcgtt acagcagcag atgaaaatgt ggctgatatg gaaagaacaa tagaaggatt
    25081 tagaggattt acatcatgtc taaatggttc taaggaaaaa ggtatagtat atggtgtagg
    25141 tgcttggaat gtaggtgata ttaagggaag taaggcaatg aaacaggcat atgaaatggg
    25201 taagttggcg tagtatgttt gtataaaatt gtataaaatt gaatatattc ttatcatttc
    25261 aacaataaaa agaggcttat ctgtattttg agacagcctc tttgtgctat atcatttatt
    25321 tttattctat atccttatta taccctttat ttttttatgc ctatttaagt atctgttgtt
    25381 actcatatat tttaataatt acccttaaaa aactatacag attcctgcat aatattattt
    25441 tctttagtaa ttaatttttc taggtttttc caatccattt cttccatatt tgcaaaataa
    25501 tgaccacttc ctatgcaagt caggtaagac tttccatcta attcaaacga agaacctcca
    25561 atacactttg aattacattt cttacaagta cgagttttag ttatactatc tttaatagtt
    25621 ttagagcatt cctccacagc atctttatat gtatttagat gaaaaagatt tgctttaact
    25681 ctccattttt tttcattaca ttcaatagta aataagctac gttttggttt cttagttgaa
    25741 tatactattt tgtaagaacg agcacctgtt tttggttcaa ttttacaatc taatgattta
    25801 ccaagttcat gtaaatgaca tgcaatatca taatctttac cacttagtac ttctaacatt
    25861 tcttctgttg tttctgctgt taattcactt ttgctcatat taaaaaaatc cccctatata
    25921 aataataatt tcatatgaaa ataattaata taagtatatt aaataaaact attaaaatca
    25981 ataaaattac tgcaatctta cattattggg catagaatga gagctaatga tttattagaa
    26041 ataagactta atagtatgaa aatttactta gacgtggtac aattctataa aagatttaaa
    26101 actaggaaag gagaaagttg ttgagatacg gccaatataa caatttattt ataagttgta
    26161 aatttattat gagattaatt agattgttaa gtaatactat caaaaggtag ttttttggat
    26221 tttcagcaac agtggtagat attgataatt ttttggttat tattatattt agctgttata
    26281 ttttcaatag gctctatgat aactagggta aataaggtat gggtaaaggt tttattattt
    26341 ataatagtgg gattgtcaac tatagttctt tcatatgtaa taacttgatt atgcaaaatt
    26401 ctatgaagca ggaaccctgt gactttagtc atgggaggtt cagaatttaa ggttaaaaaa
    26461 taactttaat ctgtaaattc tcaaaacaag aattctcatg ttggtaaatt tctagatatg
    26521 agaaaaaaat tgacatcata aaaaagtaat tttagagcgt ttttttacga aaaaattcag
    26581 aagtaactat tttgaatgac ttcacaaaat tttatatata aataaagttt ggcacgttaa
    26641 ttgctttaat aaaagttaac aagacacaaa taaacataat catctaagta ttgattattt
    26701 gaagttttag atttgaagtt taatctaaag gttcgtatta atattggtgt ttaggaaatt
    26761 ttattttcct gtaaatataa cttaataata ggaggtgaag tcatgtcaaa agtagtaata
    26821 gctacagaga aagctccagg agcaattgga ccatattctc aagcaattca agttggaaac
    26881 atagtttcta catcaggtca acttccaata gatatgaaga ctggcgagtt agtatctgac
    26941 ccaaaagaag caacaaaagc ttctttagat aatgtaaaag caatattaga acaagctggt
    27001 acaagtatgg ataaaatatt taagactaca gtattcgtaa aagatttaaa ggattttaat
    27061 gatattaatg aagtatatgc tacatatttt aaagaaaatc caccagcaag gtcatgtgta
    27121 caagttgctc aattaccaaa agatgcagtt ttagaaattg aagcaatggc agtattataa
    27181 gtattataac aataaaacca ataacaaaac taataaaaat taaaaactaa taaaaaaagt
    27241 ctacaattaa aaaagtttat attaaaaaag tctatataaa aatctatcta aatatttaac
    27301 tattgagctt ttctcatata atgtatattt ttatgtcaaa aatatatttg ttatatgagg
    27361 aaagcttttt tttattattg tattgtgatt tattttattg tctgcatatt ataattaagg
    27421 ttactatata aaacaaactt tttataaatt aatattgtct gttttaacgc aaatatataa
    27481 ataagtaatg ttttatatat attttaaaga gtgactatat gttaaaatat agctaaactg
    27541 ataatttggt aaattaaatt ctaatggggg aaactatgtt aagtaaatta aaagaatttc
    27601 aacaagaaat gataaaatac actgaaacag tagctagtgt gttagatgta gatattgaaa
    27661 tagttgacga tagattaata agaattagtg gtacaggtct gtataaatca aagataaatg
    27721 aaagcgttgt tactgagggc tttatttatg acaatgttat acagacaggt caggaattag
    27781 tcgtattgga tatatgtgat aatcagcttt gtatagaatg ttcccattac atgaaatgcc
    27841 taaataaagt aataatagca gttccaataa agtataataa tcgaactata ggagtaattg
    27901 gtgctattag tacagataaa actaaaaaag tagaaataag tgcaaaaata gataattatt
    27961 taaaatttgt caatcatata tgcgatttaa tttctatgaa aatagaagaa catgaagcgt
    28021 caaaaaatag cagtagaaaa atggatatga tgatagaaat cattgaaaat gttgaaaaag
    28081 gcgttataat actagacata aatagcaaaa tatcttatat aaacaatata gctctaaaaa
    28141 aattagatat agataaaaat ataatagaaa atatagtaaa tatagtatct gttgaatctt
    28201 cctcaaatgg acatgaatta ctagaaattg atattgataa caaaatttat aatataaatg
    28261 ccaaaataat tccggtatat ccatatataa atcaatacga caaaataata atatttgaca
    28321 agacatatat caatcataaa ggacatggaa aagtgaatag tggctgggga aatagtgata
    28381 tagagtctat aattggaaat tcagaagcaa tgttaaaagt aaaagagaga actaagaaat
    28441 tagcaaagtc aaattcaact gtacttataa ctggggaaag tggtacgggt aaagagctaa
    28501 ttgctagggc tatacatgct gaaggtagca gatggaacaa gccatttatt gcgataaact
    28561 gtgctgctat tccagaaaat ctccttgaaa gtgagctatt tggctatata aaaggtgcat
    28621 ttagtggagc tagtagtgga ggtaaagtag gtaaatttga acttgcaaat gaaggtgtta
    28681 tatttcttga tgaaatagga gatttgtcaa tgcctttaca agcaaagtta cttagagttt
    28741 tacaagagag aaaatttgcg agaattgggt caaataaact tatagattta gatataagag
    28801 tgattgctgc tacaaataaa aacttattga aattagtaaa tgaaggtaag tttagggatg
    28861 atttatacta tagattgaat gtaattccta taaatttgcc acctcttagg gaaagaaagg
    28921 atgatattga ggcaataatg atgaagtttg cttctaaata ttctttagaa ttaggtattc
    28981 agttaaataa aatagaagaa aatgtaatga atatgttgat taactataat tggccgggaa
    29041 atataaggga actggaaaat gcagttgaat atatgatgaa tttggttgga gatgatggaa
    29101 taatatataa agatatgctt ccattagata ttttaaacta ttataatatt aatggaaata
    29161 tttataaaaa taaggatgta aatataatat ttgaagatga tatagttgga gggatagtag
    29221 agaatcaaga aaggatactt tcaattaaag aattagagtt gacttacata aacaagttgt
    29281 tgaataagta tggaagagat acaaagacga agaagaagat agcaaaagac ttaggcatag
    29341 gtttggctac actatataga aaattggaag aggaacagta gtaatataat attaaaatga
    29401 taggtatttg tgtgttttta tagataggta tttttacttt atggtggagg tgttattatg
    29461 caatatgcga tagagctgta ttatgataaa aaaacggaaa agcaactttt tgatttatct
    29521 aaaaaaatcg ctgatgaaaa aattagtacg aagtatttag aatggaaaac aagacctcat
    29581 cttactttgg cttgtttcaa tgatgtggat gaggcttgtt gtatagacaa gctaaagggg
    29641 tttgcacaaa accataaggt aatacctgct tacattggtt cagtaggcat gtttaatgat
    29701 actaaaacgg tgtttgtatc acctataatg aatagcagta tgtagcagtt tcagagagaa
    29761 ttatatgagg tactacaaga ttttgattca aatggttggg aatggtacca ccccaatcgt
    29821 tgggtacccc attgcacaat agcacttact ggagatgatg aaagtgaggc tttttttcgg
    29881 gcaagtgatg tattactgcg tgagttcaaa aaaataagtg gtgagtttgt ctcaatagga
    29941 ttggttaaaa ttacatttcc tgtggaagaa atttttacag tgaatatgaa taggtaactt
    30001 taactctgtt gtggatgtta tgaatatttt gtatagtgca tttcatcaga taaatgcgaa
    30061 gttgcgaatt tcgacagctc aatagtcgca agtagggcac aatggtacac atattgcact
    30121 tttgcttatt ggggggacgc ccatgtaaac ttctgtaaga agctatgaaa agatttaagc
    30181 cattttggag gctttgggta ataaagaata tttgggtaat aaagaatata aagtatataa
    30241 atttatagag tttatgcgta agaaggttat agccatatat ttatagaaaa taaaagtagt
    30301 aatatatctg acaaaacaat agacatataa aaaataagag cataataaag gaagaattaa
    30361 gataaataaa cgatttctcg aattgataat tctcgtattg ataaaaattt cccaatatga
    30421 gaattttttc ttttataaat cttatttttc acactcaaaa tttataaact ttaaagaaat
    30481 tttataaaag tatgtaattc caacataaaa aaattgtttt tagaataaat aatattggca
    30541 cggtaattgc tttgctataa ggcacaaaga taaaaaatta aaatgacacc gaaaaatttg
    30601 aaaggggaat ataaaataaa tgagagaaat aaaatggaaa ttaaataact tacctaaatc
    30661 agaggaaaaa gaaaaaggaa ttgaattttt aaaagatgaa gagattgcaa aggctaaagc
    30721 ttttcatgaa agctttccac aatatgaaaa aactccttta gttaaattag ataacttagc
    30781 aaaatcacta ggcgtaagcg gaatatatgt taaagacgaa tcttatagat ttggtttaaa
    30841 cgcatttaaa gttttaggtg gttcttactc tatgggtaga tacttagctc aaagattaga
    30901 tacagatata tctgagttag ggtatgataa attaacttca aaagaaataa aagaaaaatt
    30961 aggagaaata actttcttta cagctacaga tggaaatcat ggtagaggag ttgcttggac
    31021 agctaataaa ttaggtcaaa aatctgttgt attaatgcca aaaggttctt ctgaatttag
    31081 attaaataaa atcaaaggag aaggtgcaga tgctagtata acagacttaa actatgatga
    31141 tgcagtaaga ttagctaatg attatgcaga agctgatgac catggagtaa tggttcaaga
    31201 tacagcatgg gacggatatg aagaaatacc agcatggata atgcaaggat atggaactat
    31261 ggctaaagaa gctatagaac aattaaaaga atatggagta gatagaccaa cacacgtatt
    31321 tgttcaagca ggagttggtt cacttgctgg tgcagttcaa ggatatgttg cttcaatata
    31381 tgatgaatgc ccaataacag tagtagtaga ggctgatgag gctgactgtt actataaatc
    31441 agcagaagct ggagatggaa aaccaagatt tgttggagga gatatgccaa ctataatggc
    31501 agggcttgct tgtggagaac caaacactat aggttttgaa gtgttaaaaa attattcatc
    31561 agcattcgtt tcagcaccag actgggtatc tgctaaaggt atgagaatat taggaaaccc
    31621 tctaagagga gatgagaaag taatctctgg agaatctgga gcagttacaa ctggagcatt
    31681 agtttcaatc cttgaaagtg aagatttaaa agacttaaga gaagctttga aactagatga
    31741 gaactctaaa gtattattaa taagtacaga aggagatact gaccctgata aatatagaga
    31801 tatagtttgg aatggtgaat gccaaagtaa ataaaagact taaggaaaag aaaatcttaa
    31861 caaataaatg aaaaatttaa ggaaaataaa aacttaaata agtgaaaact ttaaataaaa
    31921 taaaaactaa aacaaatgaa aaatctaaat aaatgaaaaa gtaaagcaaa taaaaaagta
    31981 aagcaaatga aaaattcaaa ataaaaacca aataaaatta aaataaagtc taaataaaaa
    32041 tttaataaat aaaatataaa gaaataaaaa atcaatagat taagtgaaat gctaataaat
    32101 taaaaaaatg aaatatcaaa ataaaacgtc aacaaattaa aaattatatg ggggaatatc
    32161 aaaatgttaa atgaaacaag aaaaaaacaa ttaactgaag tatgccaaga cttaataaga
    32221 aatgctagtt attcaggtca agaagaaaac gtagtaaaag ctatagaaga aaattttgct
    32281 ttactaggtt ttgatagttg gtcaagagat agatatggaa atatagtagg atgtataaaa
    32341 ggaaataaac caggtaaaaa aatattattt gacgggcata tagatacagt tccagttcca
    32401 gatgcaagta aatggagtgt tcctcctttt gaagcgagaa tagtagatgg gaaaatatat
    32461 ggtagaggaa cttctgatat gaaggggcaa gtaagtgcta tgatggctgc tgtttcatac
    32521 tttgcagaag atactaacaa agactttgaa ggtgaattgt atgtagcagg tgttgttcat
    32581 gaagaaatat ttgaaggtgt ttcagctaga gagataagta aggctgttca acctgattat
    32641 gttgtaatag gtgaatcttc agaattaaac ctaaagatag gtcaaagagg tagaggagaa
    32701 atagtagtag aaacttttgg taaaccagct cactctgcta atccagaaaa aggtataaat
    32761 gcagtttata aaatggctaa tgtaatccaa aagatacaac aattagtacc tccaactcat
    32821 ccagttttag gtgatggaat attagtttta acagatataa aatcttctcc atatccagga
    32881 gcatcagtag tgccagatta ttgcaaagct acttttgata gaagattatt agttggagaa
    32941 acaagagaag gcgtattagc tccaatacaa gctttattag atgaaatgat gaaagaagac
    33001 ccagaattaa atgcaaaagt ttcttatgca gttgaaaaag ctgattgcta tacaggaaat
    33061 actatagaaa gtgaaagatt cttcccaggt tggctatatg atgaagaaga tgaatttgtt
    33121 caagcagcgt ataagggatt aaaagaagct ggaatagatt cagaaataac tcaatactca
    33181 ttctgtacga atggaagtca ctacgcaggt gaggctggaa taagaactat aggatttggt
    33241 ccttctaagg aaaatcttgc tcatacaata gatgagtata tagaattaga ccaattattc
    33301 aaaggtgcag aaggatatta tggtatatta aaatcagttt ataaaaaata attaggagat
    33361 ggttttgtga gaattttaat aaaacatggg cttatagtag atggaaataa aacaccagca
    33421 tatgaaggag atattttaat agaaaacgag aaaatattaa aaatatcaca agatttaaac
    33481 gaagaagctg acaaagttat agatgcaaaa ggtagagtta tatgtccagg tttcatagat
    33541 actcatagcc attcagacct agttatttta gttaatccat ataatgaggt aaagattaga
    33601 cagggaatta caacagaagt gttgggccaa gatggtatat ctatggcccc tctacctcaa
    33661 gagcatatat cttcttggag aaaaaattta gcaggtctag atggtgaaag tgatgaaatt
    33721 gattggaaat atgaaacaac agagaattat ttaaaaatga tggattacaa tggggtaggt
    33781 ttaaatgaaa cttatttagt acctcatgga aacgttagaa tggaagctat gggacttgaa
    33841 gatagacctg ctactaaaga agaaattcaa aaaatgtgtg aaatcacaga gagagaatta
    33901 aaagcaggag ctataggatt gtctactgga cttatatata taccttgtgc atactcattg
    33961 actgaagaaa ttatagagat gtgtaaggta gtagcaaaat atgatggagt atttgtagtt
    34021 caccaaagaa gtgaagcaga tacaatttta acttcaatgg aagaaatcat agaaattgga
    34081 aaacaatcag gagtaaaagt tcatttttca cattttaagg tttgtggtaa agcaaactgg
    34141 aagtatatac cacaagttat agagctttta gaaaaagcag aaaaggaagg aataagagta
    34201 tcttttgacc aatatccata tgcagcagga agtacaatgc ttggagtagt tttacctcct
    34261 tgggcacatt ctggaggaac tgataagtta atagaaagat taagtgatga aaacgaaaga
    34321 gcaaaaatga aaaaagatat agcaaatgga atagaaggct gggataattt tatagaattt
    34381 gctggaatag accaaatatt tgtaacaagc gtaaaaacag aaaagaataa agatacaatt
    34441 ggtaagaact tattagaaat tggaaaaatg agaggaaaag acccattaga tgcaacgttt
    34501 gatttattaa aagaagaaga aaatgcagtt gggatggtag atttctatgg attagaagaa
    34561 catataatag gattcatgaa aagagacgag caaaatgtgt gtacagatgg tcttttagca
    34621 ggtaaaccac atccaagagc ttatggttct ttccctaaaa tactaggaag atatgttaga
    34681 gagttgaatg tgttaacaat cgaagaagct gtttataaaa tgactaaaaa ggcagctaca
    34741 agctttagta taaaagatag aggcgaatta aaagaaggat attttgcaga tatagttata
    34801 tttgacaagg atacagttag tggttgtgat gattttatca actctatgca gtaccctaca
    34861 ggtatagatt acgtaattat aaatggaaat tgtgttgttg aagaaggaaa atacaataaa
    34921 attaaagcag gaaaagtact taaaaattaa atcttagctt taaggaggaa taaatttatg
    34981 attttagtgg ggaatgggag gctaataagc caagattctt taaatcccta tatggaagat
    35041 ggatgtgtag taataaatga taatctaatt gaagatatag gtactacaga agaaatgaaa
    35101 gctaaatatc cagtacatga atttatagat gcaggcaaga aaataatcat gccgggtcta
    35161 ataaattcac atatgcatat atacagctca tttgctagag gaatggcagt tccaggaaaa
    35221 ccttctgaga actttttaga gatactaaac aatttatggt ggagacttga caaacagctt
    35281 acactggaag atacaaaata cagtgcatat gcaacgtata tagaatgtat aaaaaatggt
    35341 gtaacaacag tttatgacca tcatgcaagt ccaaatgcaa ttgaaggcag tttgtttaca
    35401 atatcagatg ttgcaaagga tttgggaatt agaacgtgtc tttgttatga agtgtctgat
    35461 agagatggtg gaaatacaat ccaacaaggt atcaatgaaa acgtaaactt tataaaatac
    35521 gctcaaaaag atgatacaga catgataaaa ggaatgtttg ggcttcatgc tgcatttaca
    35581 ctatctgatg atacattaaa taaatgtgct tcagagatgg ctggtcttga tacaggttac
    35641 catgtacatg ttgctgaagg actagcagac ttacaatata atgtagataa atatggaaaa
    35701 agggttttag aaagattcaa tgattttgga gtactaaaag aaaaaacaat agcagttcat
    35761 tgtatatata taagtgcaga agaaatcagt cttttaaata aaacaaatac atttgtagtt
    35821 cataatccag aatctaatat gggaaatgca gttggatgtt ctcctgcaat agagttatta
    35881 aaaaggggag ttacagtagg attaggaact gatggatata ctagcgatat gtttgaatca
    35941 ttaaaagttg ccaatataat tcacaaacat cacttaaaag acccaagagt tggatttgaa
    36001 gaatcacaaa caatgttatt tgataacaat agaaaaatag ctaaagctta ttataaaaat
    36061 gacctaggag tacttaaaaa aggtgcttat gcagatgtta tagtagttga ttatatacca
    36121 catactccat tgacagaaaa tacaataggg ggacacacta tttttggaat gagtggaaga
    36181 agcgtagata caactattat aaatggaaag gtacttatga aagatagaaa gttaataaac
    36241 atagatgaag aaagtattct agcaaaaagt agagaattaa gtcaaaatct atggaataga
    36301 atttaaataa ttgattatgc aaaattctat gaagcaggaa ccctgtgact ttagtcatgg
    36361 gaggttcaga ttaatataca agtaagaaac aagaatttag atgttaaaaa cagagcttaa
    36421 acaaaaatag gaatttaaat gaagtatata aataaaaaat atacggaata gaatattaaa
    36481 ttaaatatag aagggagacc caaaatgtct cgagataaaa gccctaaaat agctccagta
    36541 gatgaaaggt tacctgtatc caaatcttgg gtattttcac tacagcacgt tttagcaatg
    36601 tgtgcaggag cagtagcagt tcctatgatg ataggaagtg ctgcacgact agatcatgct
    36661 agtattgttt ttttaataaa tgcttgcttg tttatggcag gtataggaac actcatacaa
    36721 agtgttggta taggaaatat tgtaggtgct aaaataccag taatagaagg aactagtttt
    36781 gccgctgtgg caggtatgac agcaatagcc tcatcatata gtgattcaca agttgcaata
    36841 cagacagtat ttggctctgt aatagtagct ggattgtttt gtatattact tgctccagta
    36901 tttgaaaaat taataagatt tttccctagt gtagttactg gaacagttgt tatggtaata
    36961 ggtatttcac tatttccagt tgggataaaa tggataactg acaacaaatt ggaagcggtt
    37021 gaaccaaact cattagcatt agctttagca gtattggcaa taactttact ttcatttaaa
    37081 ttcttaaaag gaatttggaa tagcacagct atattggtag gtatagtact tggtacacta
    37141 ctttccatgg cagttggaat ggcagatttt agtgcagtaa atggagttgg ttggcttaac
    37201 ttaaatgtac ctttaaaatt tggaatgcct agatttgaac catctgcaat actatcattg
    37261 tgtttgatta tgttggtact tatgacggaa tcagttggta atatgatagc tattcatgaa
    37321 atggtagaca aagaagtaac aggtaaaaat attaaaagag gtctatttgg agatggaata
    37381 tctacagcat tatctggaat attcaattca tttccaataa ctccatttgg tcaaaataca
    37441 ggaatagtag gtcttacagg tataaaatca agatttatag cagtttacgc aggggttata
    37501 ttgctagtac ttggattctt accaaaattt gcagcaataa taggagcaat acctaagcct
    37561 gttcttggag gcgtaggatt tgcaatgttt ggaatggtaa tggttggagg agtaaaaact
    37621 ttaagcaaag tgaaatttga tggaactaaa aatggggtta tagtagcagt tagtgttggt
    37681 ttgtctatga taccactaac aaatcctgat ttctatcata atttcccaac ttgggtacaa
    37741 acaatattcc atagtggaat aacaacaggt agtttatcag cagtgctttt aaacatattc
    37801 ttcaatgaac taggttctaa aaaagaaaaa aataataaag ttgaagaagc aaaataaaaa
    37861 taaaacaagt aaaaatctat attgacttaa agtcattaag tattaataaa aataattcag
    37921 agatattaat aattcaacaa aactatctta tattttaaat aatataaaaa agtaaataaa
    37981 taacaaatac aaataaaaga atacaatcta aatattaaaa atatttaatt taagggagag
    38041 ataataatat ggctaattta aaagttaatt tatatggaat ggaattttta aatccaatta
    38101 tgacagcggc aggacctgga gcaaaagatg gtgctctctg tcaagaggca gcaaaaggtg
    38161 gagcaggagc tatatgtaca aaaactatat ctgtattacc agctgatgta ccaagaccat
    38221 gtatggcaaa aactaatagt ggttttttaa acacagaatt atggtcagag cttccaaaag
    38281 agcaatggat agaggaagaa tacaaaaagg taaaagaaac agggcttcca gtaatagtaa
    38341 gtatgggata ttcagcagag caaatagctg aggttgttcc tttagtaaag ccttatgcag
    38401 atgcagttga attatctact cattatgtag gtacagacgt tgcaccaata gtaaatgctt
    38461 taaaagctgc aaaagctgtt ttagatgtac cagtatttat gaaaatgagt cctcatactg
    38521 acattcaaac tattgcaaaa gctgttgaag aagcaggtgc agatggactt gtaatgataa
    38581 actcatttgg accatgtatg gcaatagatg taaatacagg ataccctata atgggaagta
    38641 aagctggata cggatggtta tcaggtgcac caataagacc tctagcaata agatgtatat
    38701 atgaagcttc tcaagttgta aatataccaa taatcggtgt tggtggaatc acttgtggaa
    38761 aagacgtggc tgaaatgatg atggctggag caagtgctgt tcaagtatgt acagaagcaa
    38821 tccttaaagg gccttctatc tatggaaaaa ttgctaaaga attaaatgaa tttttagatg
    38881 agaatggata tgctgatgtt aatgaaataa aaggactaac tcataagaaa atggcagcta
    38941 gagggtttag aactcattca gtagctccag tagtggatga taacaagtgt atcaaatgta
    39001 agcaatgtgt aacaagctgt gtatacgaag ctttagaagt tacagataag ttgaacatag
    39061 atgcagacaa gtgttttgga tgtggattat gtgtaactag atgtccaaaa ggagctttaa
    39121 gcatctgcta caatcaagta gaagctatga atgagtaata attatataga attttggtaa
    39181 ttaacttaga gtaataggta ttaggataat aaaatatatc agataaacaa acacacatta
    39241 tgcattaata atacagtcta aagttaataa ttttattata ttgatttaac taaatattag
    39301 tttagctgaa tattaattta attaggtatt ggtttaatct taatatagat ttactgcgta
    39361 taagtgaact aactagatta ctttaagtta attttttcat ataaaataaa ttgatacatt
    39421 acaagtaagg tggagtctat atgggaattg ttttaaaagg tgggaaagta gtaacaagtg
    39481 aaaaaactta ctgtgcagat gtaagaatag aagatgaaaa aatagtcgat ataggtcttg
    39541 atatatcaaa agaaaatgat gaaatagttg atgttagtga ttcttatgtt ataccaggag
    39601 gtatagatac acacacacac tttgacttag atacaggcgt tactaagaca gcagataact
    39661 ttgagacagg aacaaaagct gctatagtag gaggaactac tactatattg gattttgcta
    39721 cacaagctaa aggatgttct ttgaaagacg gattagaaga atggcatgaa aaagctaagg
    39781 ataaatccta ttcagattat ggctttcata tggctataac agattggaat gactcggttt
    39841 gtaatgaaat ggaggatatg gtgaaagagg gagtatcttc atttaaactg tatatggcgt
    39901 ataaaggaag ccttcaagtt gatgatggtg taatctttga agcattgaga aaagcagaag
    39961 agataggagg gattattggt ttccattgtg aaaatgggga cataatatgt gaacttgtgg
    40021 ataaagcaaa gtcagaaaat aatttatctc caaaatatca tcaattaact agaccagcta
    40081 taatggaggc agaagctaca tcaagactta tgaagatagc tgaagttgca aactctaaag
    40141 tatatgtagt tcacttaagc tgtaaagaat ccttagagga ggttttaaaa gctaagcaaa
    40201 gaggtgtgga tgtaatagtt gaaacttgtc ctcaatacct tctattaaat gatgaattat
    40261 atgatttaga tggatttgaa agtgctaagt atgtaatgtc accaccttta agaaataaag
    40321 aaaataatga aatattatgg aatgctattt tagataatca aatagacact ataggaacag
    40381 accattgttc ttttaattat aagggacaga aggaacttgg gataaacgat ttcagtaaga
    40441 taccaaatgg ttctccaggt gtagaacata ggatgagtct tttatacaca tatggagtct
    40501 gtaaaaataa aatatctatc aacaagatgg tagaagtaac atcaactaat gcagcaaagt
    40561 tgtttggaat gtatcctaat aaaggaacta tagaaatagg cagtgatgca gatatattag
    40621 tcttaaatcc aaacaaaaaa tccaaaatag aagcaaaaaa acaattacaa aatgtagatt
    40681 atacacctta tgaagggtat gaagtggatt gtcaaataga acatgtttat ttaagaggaa
    40741 aagaagtagt tagagatgga gaattaattt gtaatactcc aagtgggaga tatataaata
    40801 ggcgtctaac aaagggggct agagaatggt agaatttata ttaaatggag agaaagttgt
    40861 tgcaaaagaa ggcaaaaaac ttttagactt ccttagagaa gatatgaata taacttcagt
    40921 taaaaatgga tgttcagaag gtgcttgtgg aacatgtatg gttataatag atggtaaagc
    40981 tactaaagca tgtgtattta aaactgataa attagctggg aaaaacataa caactataga
    41041 gggattatca gagagagaaa gagatgtttt tgcatattct tttgctagtc aaggagctgt
    41101 acaatgtgga ttttgtattc caggtatggt aattagttca aaagcactta tagacgttaa
    41161 taataatcct actgaagatg atataaagaa agctttaaaa ggtaatatat gtagatgtac
    41221 agggtatgta aaaataatta aagctgtcga tttagcggca aagattttta gagaaaatat
    41281 agatgtgcca aaagtaaact gtaagggtct agtaggtgaa aatcttccta gagttgatgc
    41341 agttggcaaa gcacttggat attctgaata tgtagatgat atgagaattg agggtatgat
    41401 ttatggttca gcagttagag ctaagtatcc tagagcttta gttaaaaaaa tagatataga
    41461 agaagcaaaa gcttatccag gagttgttgc agttctaaca gctaaagata ttccaggaac
    41521 tataaaggta gggcacttaa agaaggattg ggatacttta ataccagaag gtgaaataac
    41581 tagatactta ggagatggaa tagttttagt agcagcagaa actagagaag cgttagaaga
    41641 ggccaaaaag ctagttaaga tagattatga ggaattaaca ccattaagta ctacagcaga
    41701 tgcacttaga gaagatgctc caaagataca tgagacaggg aatatacttg ttaaggaaca
    41761 tttagtaaga ggaaacgcta aagaaaagat agaaaattca aaatatgttg taactaaaag
    41821 atatactaca cctgctacag aacacgcatt tttagagcct gaatgtgcag tagctggtcc
    41881 ttatgaagat gatggagtaa taatctattc gacagaccaa ggggtgtttg ccactcaaca
    41941 tgaatgtgca gatatgttag ggttaccata tgagaaggta agagttataa acaaaatggt
    42001 aggaggaggg tttggtggaa aagaagacat gagtgttcaa caccatgcag ccttacttgc
    42061 ataccataca aagagatttg taaaagtaca gttaacaaga aaagaaagta ttattgttca
    42121 tccaaaacga catgccacag aaatggaatt tacgacagca tgtgatgaaa atggatactt
    42181 aacgggtatg aaggcaacga taatatctga cacaggtgca tatgcctctc ttggaggacc
    42241 agtgctacaa agactgtgta ctcatgcagc aggaccatac aattatcaag atattgatat
    42301 aacaggaaca gcagtatata ctaataaccc tcctggagga gcatttagag gatttggagt
    42361 tacacagagt tgttttgcaa ctgaatgtaa tctaaattta cttgctgaaa tggttggaat
    42421 atctccttgg gagataagat ataaaaatgc aataagacct gggcaagttt taccaaatgg
    42481 tcaatttgca gatgaaggaa cagcaatggc agagacacta gaagctgtaa aagaagagta
    42541 tgaaaagcat ccacgtgcag gtatagcttg tgctatgaaa aatagtgggg ttggagttgg
    42601 tatacctgat gttggaagat gtagattggt gataaaagat ggcaaagttc acataagaac
    42661 aagtgcttct tgtataggtc aagggcttgg tactgttatg actcaagtag tttgtgaaac
    42721 tgctaaccta cttcctgaaa atatagttca tgaagttcca gatactcatt taacaccaga
    42781 ctctggtaat actacggctt caagacagac agtatttacg ggagaagcta ctaaggttgc
    42841 atcaaataag ttaaaagaag ctttagactc tggcaaaact ttacttgatt tagagggaga
    42901 agagttttat ggagaatatg aaagtattac agataaaatg ggttgtgaca aagaaaatcc
    42961 agtaagtcat gttgcttatg gatatgcaac acaagttgta attctggatg aaaatggaaa
    43021 actagaaaaa gtagtagcag cacatgatgt tggaagagct ataaatccaa tcagtttaga
    43081 aggtcaaata gaaggtggag tagttatggg tcttggatat gctctaaccg aagattaccc
    43141 agtagttaac tctattccta cagctaagtt tggtacatta ggtttattta aatctacaga
    43201 tataccagaa atacaagctt taatagttga aaagaacaaa gatttattgg cttatggggc
    43261 aaaaggtgta ggagaaattt gtacagttcc tactgcacca gcagtacaaa atgcctatta
    43321 tgtatttgat aaagaattta gaactgagtt accactacaa aatactccat atagtaaaaa
    43381 aaagtaacaa aaaaaagaaa tattaaataa aaaaatagat gaaattgtaa ataaaaaaaa
    43441 cccttttgca taaggtatat tttaaatgat agaatataca tatacaggag ggtttttctt
    43501 ttataaaata agtttaataa aattgagagg tgagcagaat gaattttaat aatagtctag
    43561 aattaactca gtctcagaag ctaataatga ctacacaact taagcaatca ttatcaatat
    43621 taaatatgag taagttagag ttagaagaag agataaaaag agaagcagag aataacccat
    43681 tagttgaagt cgagaaaagt ggggagataa attgggaaga gtatataaaa gacatggata
    43741 ggtcaagacg tttagataga acagagataa gctataatcc tgataatgaa gttaatctag
    43801 aaaatttagt taaatattcg tcaaatttat atgaagattt aaaatttcag ataagcctgt
    43861 ataaacttac agacaaagaa ttggaagtct gtgagtatat aattgatagt ttagatgagg
    43921 atggatattt aagaactgag gaaaaagtaa ttgttgatac cttaaagata gatgaagaac
    43981 tttttgaaaa atgtttaatt agtgttcagc agttggaacc tagtggagta ggggcaagaa
    44041 gtctttcaga atgtctgatg atacaaatgg ctagtttagg aatatataat gaaattttag
    44101 aggaaatcgt tacaaaagac ttaaatttaa ttgcaaataa taaatacaaa gagataagta
    44161 aaaagtacaa tatgtcatta caaaaatgtg ttgaactaat aaatataatt aagacattag
    44221 accccaaacc aggaagaact tgttcagtag agaaaagtgt atacattcaa cctgatgtaa
    44281 cagtagaaaa aattgatgat gaatttattg tatatttaaa tgagaaagat tcttatcaaa
    44341 taagaatcaa tagttattat aaagacatat taaaaaattc acagtctgat gaaagtgcaa
    44401 aagaatttat aaaagaaaga ctaaattcag caacaggact tatgaaaaat atagagagta
    44461 ggaaaactac agtacttaag attgcagaag agatagtaaa agctcaagat gaatttttga
    44521 gaaaagggac aaaatatata aaacctttga aaatgaaaga catagcagaa aaattagaat
    44581 ttcatgaatc tactataagt agaggtgtaa atggtaaata tatgctaaca ccttttggcg
    44641 tatatgaatt taggtacttt ttcagtagcg ccattgagac tgagaataat gaaatggcat
    44701 ctagtacaag cataaaaaaa attataaagg aaacaataaa agacgaaaat aaaaagaaac
    44761 ctttaagtga tgaccatata tctaagttat taaaagaaaa aggaatcaat gttgcaagaa
    44821 ggacagttgc caaatatcgt gaagaattag gaatcccatc ttcaagtaag agaaaagaat
    44881 actaataagt aaaagacaaa tatcagtatg gtaaggggaa aaggctaatt aagagatgaa
    44941 tgtagattgg gctaaaaatt ttataaagta aaataatata atatgaaaaa gtattaaaat
    45001 gctataaggt attaaaaaac tggggaacat taaaacatta agatagcaaa gagcataaaa
    45061 aactaaagaa gattaaaaca ttaaagaaca ttagagcttt gaggaatatt agaactttaa
    45121 agaatattaa aatttataaa atataaaaat actaaaaaga attgaaaaac tggggaacat
    45181 taaaatattg taaaatgcta aaaaactaga aaacataatt aaataagtgc ttataaagaa
    45241 tgattaaatt aaataagtgt ttgtaaagaa taattagata ttagaaaaag aagaatacct
    45301 agataaataa actattatta aatttaataa tcacaaaagg aaaacacgta gggtataaat
    45361 aaaaatctta actagataaa agtaaactta gttgtagata aaaataacta gaaataaaat
    45421 tatttatagt aataaatatt agaatgaaaa tataaaaaaa tatatataaa aagccttata
    45481 taaaaagaaa aaatactgtg atttgaaaat ggtggagaaa tattttataa aataaaaaaa
    45541 taaaaaagtt tttgaaaaac tattgaaatc tatacgcaaa tcctataaaa taaaaatatc
    45601 ttgtgggact taaataaaca ctagggacaa aaaaagtccc gaaaggaaag caataaggag
    45661 tatgttatga agaacttatt agaaattcaa aagaaattga ttccacaggc aattgaactt
    45721 atggaaagaa gatactctat tttaagacaa atatctttaa gtgaaccaat aggtaggaga
    45781 acattatcaa atgtattaga gattagtgaa agaataatca ggtcagaaac tgagttttta
    45841 aaagaacaag gacttataga cgtagctgta tctgggatga ctatcactaa agaaggttct
    45901 gatttgttag ataaacttaa ggacatcatg agtgacatca tgggattgtc taagttacaa
    45961 gatagagtca gagaaaagct tggaataaaa agggtaatat tagttcctgg aagctgtgat
    46021 gaaaatggaa gtttactaaa agatgtaact agatatggat gcgaatattt cttaggcatt
    46081 ttaaaagatg gagatatcgt ttcaataaca ggaggaagca ctatgttgga gttttcaaac
    46141 gttattaaaa ctgataagag atatccaaac tctacaatag ttccagctag aggaagtatg
    46201 ggaactgatg tagagatgca atctaacaat atagttgctt caataagtaa gaaattacat
    46261 tctaattgca aacttttaca tataccagat gagttggatg aaagtgcaat gaaaactctt
    46321 agccaagtgc cagagattaa gacaactttg gagtatatta aaagaacaga cattttagtt
    46381 tttagtatag gtagagcaga tgtcatggca aagagaagaa aacttccaga agacaaggtt
    46441 gatgagatat tatctaaaaa tgcagttgga gaagcatttg gatattattt caacaaagat
    46501 ggagaaatag tttacaaact gaacactgtt ggaattgatt tagaaacagt taaacaagta
    46561 aaagaaacta ttgcaatctt tgcagggact gaaaaagtag aagccttatt agctatatca
    46621 aatatgcata aaaacatggt tcttgtaaca gatgaagaaa gtgcttataa gatattgtca
    46681 ttattataaa agtcaagacc tagcacagag ctagaaaaca tacatttact ttataggagg
    46741 tagcgaaaat ggttaaagta gctataaatg gatttggaag aataggaaga ttagcattaa
    46801 gaaaaatgat ggagcaacaa gataagttcg aagttgttgc aataaacgac ttaacagacg
    46861 ctaaaatgtt agctcattta tttaaatatg atacagctca aggaagattt aacggagaaa
    46921 tagaagttaa agaaggtgct tttgtagtaa acggaaaaga aataaaagta acagcagaaa
    46981 gaaatcctgc tgacctacca tgggctgaat taggagtaga tatagtatta gaatgtacag
    47041 gattctttac atctaaagat aaagctgaag ctcatataca agcaggggct aagaaagttg
    47101 ttatatctgc accagctact ggagatttaa aaacaatagt attcaacaca aacagtgaca
    47161 tattagatgg ttctgaaaca gttatatcag gagcttcttg tactactaac tgtttagctc
    47221 caatggctaa agtattaaat gataaatacg gtatagaaaa aggcttaatg actactatac
    47281 atgcatacac taatgaccaa aatactttag atggtcctca tccaaaaggt gatttaagaa
    47341 gagctagagc tgctgctgga aacatagttc caaacactac aggagctgca aaagcaatag
    47401 gcttagtaat accttcatta aaaggaaaat tagatggggc agcacaaaga gtaccagttg
    47461 taactggttc aataactgaa ttagtttgta cattaggaaa aaatgttaca gttgaagaaa
    47521 taaatgcagc tatgaaagaa gcttcaaacg aatctttcgg atatactgaa gaaatgttag
    47581 tatcttctga cataatcgga ataagctacg gttcattatt tgacgctact caaacaaaag
    47641 ttatggaagt agatggaaaa caattagtta aagttgtttc ttggtatgat aatgaaatgt
    47701 cttatacttc tcaattaata agaactttag gatactttgc acaattagca aaatagtcag
    47761 ttttgagaaa atagaaaagt agattgacaa agtccgagtt tgtagtttaa tactacggac
    47821 tcaggacttt ttttaagaaa aaatatataa aatacaccta aataatgtta catactatat
    47881 ataaaaatat gataatatga tatatttaat agggtaaaag tatgtactat attatacttg
    47941 gcaataaaaa attactttat taaaaggaga gatatttatg tcaatgctta acaaaaaaac
    48001 tatagaagat attgatgtgt gcggaaaaaa agtattagta agatgtgact ttaatgtgcc
    48061 attacaagat ggtgtaatta ctgatgaaaa tagattgaac ggagctttac caactattca
    48121 atatttaatt tctaaagggg caaaagttat attatgctct cacttaggaa agccaaaagg
    48181 agaagctaaa ccagaattat ctttagcacc agtagcaaag agattatctg agatgttagg
    48241 aaaagaagtt gtatttgcag cagacgataa tgttgtagga gaaaatgcaa agaaagcaac
    48301 tgaaaaaatg gaaaacggag atgtagtatt attagaaaat actagatata gaaaagaaga
    48361 aactaaaaac gaagagaatt tctctaaaga actagcatca cttgctgaaa tatttgtaaa
    48421 tgatgcattt ggaactgctc atagagcaca ttgctcaaca gttggtgcag gcgagttttt
    48481 acaagagaga gtttgtggat acttaattca aaaagaatta aaattcttag gagaagctgt
    48541 tgcaaaccct gtaagaccat ttacagctat acttggagga gctaaagttt ctgataaatt
    48601 agctgttata aacgaattat tagaaaaagt tgataatcta ataataggtg gaggaatggc
    48661 ttatacattc ttaaaagctc agggttatga agtaggaact tctttacttg aaatagataa
    48721 agttgaatat gctaaagaaa tgatggaaaa agctaagaat aagggtgtta acttattatt
    48781 accagtagac gttgttatgg cagaccactt tgcaccagat gctactccaa tagtaacaga
    48841 agatgctaat gtaaaagaag attacatggg actagatatg ggacctaaga ctatagcaaa
    48901 ctttgtaaaa actataaaag aatcaaaaac tgtagtatgg aatgggccaa tgggagtatt
    48961 tgaatttgaa aactttgcaa atggtacatt atcagtagct agagctatgg cagagttgac
    49021 tgatgcaact actgtaattg gcggtggaga tagtgcagca gcagttaatc aattaggatt
    49081 tggtgacaag atgactcacg tatcaacagg tggaggagca tcactagaat ttttagaagg
    49141 taaagaatta ccaggtattg cagcattaga taataaataa actaatatat agaggagatt
    49201 gtaaaatgag aaaacctata attgcaggaa actggaaaat gcataaaaca ataaaagaag
    49261 ctttagaatt tgtaaatgaa gttaaagata aagtaaattc tgataaagta gaagctgtta
    49321 tatgtgcacc atttacttta ttaaaggatt taaaagaagc tactaagggt acaaatataa
    49381 agataggtgc tcaaaatatg cactttgaag aaaaaggagc atttacagga gaagtttcac
    49441 ctcttatgtt aaaagaaata gatatggact atgttgtaat aggacattca gaaagaagac
    49501 aatacttcaa tgaaactgat gaaactgtaa acaagaaagt tttaaaagca ttagaagtag
    49561 gaatagaccc aatattatgt gttggagaaa ctttagaaca aagagaagct ggaaaaacta
    49621 aagatgtttg taaagttcaa gttgaaaaag ctttagaaaa tgttcttaaa gatgatttag
    49681 caaaagtagt tgttgcatat gagccaatct gggctattgg aactggtaaa actgcaactg
    49741 ctgaagatgc taatgacgtt atatcttata taagagaagt tataaaagga ttatatggag
    49801 aattagctaa tgaagttaga atacaatacg gtggaagtgt taaaccttca aatgtagccg
    49861 aaataatggg tcaaagtgat atagatggag ctttagtagg tggagctagt ttagctagta
    49921 atgattacct agaccttgtt aatttctaag gagtgaaatg atgaaaaaac cagttgcttt
    49981 aattattatg gacgggtttg gatacaataa ggatgttaag ggaaatgcaa ttgctgagtc
    50041 caaaactcct aatttagata gaataaaaaa agagtatcca aacacattga taaatgctag
    50101 tggtcttgat gttggtctac ctgatggtca aatgggaaat tcagaagttg gacacactaa
    50161 cataggtgca ggtagaatag tatatcaaga tttaactaga ataacaaaat ctataaaaga
    50221 tggtgacttt tttactaata aagttttatg tgaagcaatg gataatgcaa aagaaaactc
    50281 tcttcatgta atgggacttt tgtctgatgg aggagtacat tcacatatag accatcttaa
    50341 ggctataatc aaaatggcaa aagataaagg cgtacaaaag gtgtatgttc acgcatttac
    50401 tgatggtaga gatactgacc ctcaaagtgc attagaatat gccaaagaag tacaagctag
    50461 tatggatgaa attggagtag gtgaatttgc tacagtttct ggtagatact acgctatgga
    50521 tagagataag agatgggaaa gagtagaact tgcatacaat gccatggtta gaggtattgg
    50581 tgaaaaagca aattctattg aggaagctat tcaaaactct tatgatgatg gaaaaaatga
    50641 tgagtttata atgccaacag ttattatgaa agatgataaa ccagttggaa gtataaagga
    50701 aaatgattca attatattct tcaactttag accagataga gctagacaaa taactagagc
    50761 tttagtttgt gaagagtttg atggatttaa aagagaagat attaaaaact tctttgtatg
    50821 cttaacagag tacgatataa ctatagagaa tgtgcatata gcatttggac ctcaatcttt
    50881 agccaacact ctaggtgagt atctagctaa aaatggaaaa actcaactta gagctgctga
    50941 aactgaaaag tatgcacatg taactttctt cttcaatggt ggagtagaag agccaaataa
    51001 aggtgaagaa agactattga taccttctcc aaaggttgca acttatgatt taaaaccaga
    51061 aatgtcagca tatgaattga cagataaagc tttagataaa ttaggagaag ataagtttga
    51121 ctttatagta ttaaactttg caaatcctga tatggtaggt cacacaggaa gtatagaagc
    51181 ggctataaag gctgttgaaa ctgtagatac ttgtgtagga agattgatag ataaaatagt
    51241 agagttaggt ggaagtgcaa taataactgc tgaccacgga aatgctgaat atatgttaga
    51301 cccagaaaca ggaaaaactg taacggctca ctctattaat ccagtaccat ttattgtagt
    51361 tggtcaagaa tatgaaagtg caaaattatt ggatggagga agattatctg atatagcacc
    51421 tactatttta gatatgatga agttagaaaa gccagaagaa atgactggtc attcacttat
    51481 ttcaaaatag tcgattctga taagtagata ataatatata aactacaata taaatttaaa
    51541 aataatgcag tttaggttta ttttgtaaag tttagacttt atgacataag gtttaaattt
    51601 tgtaagttct ggatattgta aggtgtagat gaagatatat tttataaaat ttacaagata
    51661 gacaatttat aaaataaaaa attacaatat agataataat gtaaataatg atataaataa
    51721 taacaagata gacaataaca atataaataa taataattaa tatatattat ataagcaaaa
    51781 attttagaat cattagttgt acattttatg tttaatgttt taaatctagt tataatattt
    51841 gtgtatttaa gattggtttt ggattttaga caagtttttt tatattatat aaattaaaat
    51901 attataaaaa ataaaataaa aataggaact tttacaaatt tacccatcaa ataaatttgt
    51961 acctaatttt caaaagcaac tttatgtact ataatataga aagtagatta aaatagtaga
    52021 cggatgttta cagtaaatga aaatttaact aatagaaaat cgaagggaga tatacaaatg
    52081 tcagttattg aattagtata tgctagagaa gtattagact caagaggaaa tccaactgta
    52141 gaagttgaag tagtattaga agatggagca atgggaagag caatagttcc atcaggagca
    52201 tctacaggag cttttgaagc tgttgaatta agagatggag ataagggaag atacttagga
    52261 aaaggtgtag aaacagctgt tgctaacgta aatgaaataa tagcacctga aatagaagga
    52321 atggatgcat ttgaccaacc agctatagat gcaatcatga tagaattaga tggaactcca
    52381 aacaaaggta aattaggagc taatgctata ttaggtgttt caatggcagt agctagagca
    52441 gctgctgatg aaataggttt accattattc caatacctag gtggagtaaa tgctaaacaa
    52501 ttacctgttc caatgatgaa catattaaac ggtggagagc atgctgataa caacgttgat
    52561 gttcaagaat ttatgatatt accagtagga gcttgttgtt tcaaagaagg tttaagaatg
    52621 ggagcagaag tattccattc gttaaagaaa gttttaggag aaaaaggatt agcttgtggt
    52681 gtaggtgacg aaggtggatt tgctccaaac ttaggttcaa acagagaagc tttagaatta
    52741 atagttgaag ctataacaaa agctggatat aagccaggtg aagacgttat gttaggtctt
    52801 gacgttgctg ctacagaaat gtacaacaaa gaaactaaaa aatacgtttt agcaggagaa
    52861 ggaaaagagt taactgctgc tgaaatggtt gctttatatg aagattggtc aaacaacttc
    52921 ccaataataa ctatagaaga tggtttagac gaagaagatt gggatggatg gaaattatta
    52981 actgaaaaat taggaaataa attacaatta gttggtgacg acttatttgt tactaacaca
    53041 gagagattag aaaaaggaat tgaaaatgga gtagctaact ctatattagt taaagttaat
    53101 caaataggta caataactga aactttagat gctatagaaa tggctaagag agcaggatat
    53161 actgctgtta tatctcacag atcaggagaa actgaagatt ctactatagc agatttagct
    53221 gttgcagtaa atgctggtca aataaaaact ggtgctccat caagaactga tagagttgct
    53281 aagtacaacc aattattaag aatagaagaa atggttggag agcaagctag atattgtgga
    53341 ttaaaatctt tctataactt aaaaaaatag ttattaaaat ggttactaaa aaagattaat
    53401 gaagagagaa tataagatat ttaagctcat cttataagat ataaaagaga taagtgttaa
    53461 ttgatttttc aattcactta tcctttttta tattttatta cttaatttta ttcatagatt
    53521 cagaaaaaat atgaataaat gtttctatct catttttatc taattgcata ggaggttttt
    53581 attaaaaaat aaaagtgaag acatattttg atttaatcat aattgtcatt gaagtattat
    53641 tgctaaatgt ggattattta ttttaaatta tgttgaaatt aaaagttata tcaatatttt
    53701 aaaatgattg ttttattttg gtggctattt ataatatata caatgagagt agattatttt
    53761 aagtgttaat ttatgtatta atttaaatag ttttctcttc taagttttat tcatttttat
    53821 attgagaata tagtaatata ttatagtaac aatatatttt tttgtatgtt atttatagca
    53881 taatagtctt gaaatggcta tattattgtg atacactaaa gagtaatgaa atttaagtaa
    53941 taaacagata ggaggggcaa gaatgagcaa cattttaatg ggtgtgcaag tagtactagg
    54001 gattttacta gtaattagca taatgcctca agattctaag aatgctttac cttcagaatt
    54061 tggtggagaa ggaagtcaag cttactttaa accaaaagga aaacaagcat ttcttgctag
    54121 agttacaaag ataacagcag tattattttt tataaatgca ttggcattac ttatagttaa
    54181 gaagtagtga tattgttgaa taactggcca ttatttaatt agtgcatttg ctagacattt
    54241 aaaaattaaa tttagttttt aataaaagta catcaacata aaaagtacgt aaaataaaag
    54301 ctggactaat gattttatat gattagtcca gtttttattt tatagattga ctatttggtg
    54361 gcgtttatta tttgtattta tatattgttt gtatttatat attttcttca aactaaaaaa
    54421 tatatagcaa cactactttc accatgttta atgagataat atacacaaag ttatattata
    54481 tagtaaaaat agtataataa tatgttttta cgcgattaaa tcagttttat atatgtttct
    54541 tataatacca atttgtgtta tcaaatatca ttaaataaga ttattctgct ttaaaataaa
    54601 tattattaaa aatactgtaa ttatagaaaa tccagaagta aatactacaa tttgtccagc
    54661 taattcactg tcagaatcca tctgttgtgc cattgtaaat gaagaaactg cagttggagc
    54721 agataacatt atcataagtg aagctaattc tacatttcta aatccaagaa aaatgctaat
    54781 tggtaaaaat atagcaggta caagtataag ttttccacat acgcccaata taatgggttt
    54841 tatatgcccc tttatagcag aaaaactaaa agagccgcct agaattataa gtgataaagg
    54901 agtagctatt ttagaaatat caccaatagt tttgtgtaaa aatgtaggta tattaatatt
    54961 taaaattagc attgtcaatc ctataaaaga ggcgattatg agtggatttg ttaagacgct
    55021 tttaatcata tgttttatat ttactttgcc acctctaaaa atttcaagaa ttacaacaga
    55081 taatacatta aacataggta caataactgc aattaataaa gatgtgactc caatactctt
    55141 atcgccaaat agtgaaattg tcacaggaat accaaatata acgaaattgc tacgaaatac
    55201 accttgtatt aatgcacccc ttgtcttatt atctttttca attttaggaa taataaagac
    55261 taagattaag aagattgaaa aaataattat aacagctaca gccataagct taaaattaag
    55321 agaaccatct aaatctgtat tgtatatatt aaaaaataat aataatggca agaaagactt
    55381 gaagcaaaca ttattcattt tatttaaagt atgagtatca aatagaccta atttttttag
    55441 aacatatcca agtgacattg tgagaaacaa aggaagaaca acatttattg ataggattaa
    55501 attttccaag gtttactctc cgtgattcta tgatttgata attcaattaa atttccatta
    55561 gtttttttca aataaataga tttcatatta ccaaagacac ctgtacattc tacaattcct
    55621 ctcttaattt gaatctcatt ttgttctaag tatttttttg gctcatcaat atttgtgttt
    55681 attttaaaac ataagttatc tctacttgct tttgaatttt taacataagg taaaaattta
    55741 taacctttta tgttaagatt tatcttaaca tttctagcat taagtttata atttttgctc
    55801 atttctttag atgaaaaacc aaatttttta taaaaattga tataattttc tatattatta
    55861 ttagtaatga ccacatgttc
//
LOCUS       DAYATN010000011        54312 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.011, whole genome shotgun sequence.
ACCESSION   DAYATN010000011 DAYATN010000000
VERSION     DAYATN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.011"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            335..1174
                     /gene="cdaA"
                     /locus_tag="ACGI8A_000654"
     CDS             335..1174
                     /gene="cdaA"
                     /locus_tag="ACGI8A_000654"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFH0178666.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1171..2352
                     /locus_tag="ACGI8A_000655"
     CDS             1171..2352
                     /locus_tag="ACGI8A_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFH0178667.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESMEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2656..3558
                     /gene="ptb"
                     /locus_tag="ACGI8A_000656"
     CDS             2656..3558
                     /gene="ptb"
                     /locus_tag="ACGI8A_000656"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050182 - phosphate butyryltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HFH0178668.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3580..4659
                     /gene="buk"
                     /locus_tag="ACGI8A_000657"
     CDS             3580..4659
                     /gene="buk"
                     /locus_tag="ACGI8A_000657"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFH0178669.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLA
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4649..5326
                     /locus_tag="ACGI8A_000658"
     CDS             4649..5326
                     /locus_tag="ACGI8A_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0178670.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            5369..5584
                     /locus_tag="ACGI8A_000659"
     CDS             5369..5584
                     /locus_tag="ACGI8A_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0178671.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5613..6692
                     /locus_tag="ACGI8A_000660"
     CDS             5613..6692
                     /locus_tag="ACGI8A_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFH0178672.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6692..7444
                     /locus_tag="ACGI8A_000661"
     CDS             6692..7444
                     /locus_tag="ACGI8A_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFH0178673.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7445..8002
                     /locus_tag="ACGI8A_000662"
     CDS             7445..8002
                     /locus_tag="ACGI8A_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFH0178674.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8172..9518
                     /gene="glmM"
                     /locus_tag="ACGI8A_000663"
     CDS             8172..9518
                     /gene="glmM"
                     /locus_tag="ACGI8A_000663"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFH0178675.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            9785..11617
                     /gene="glmS"
                     /locus_tag="ACGI8A_000664"
     CDS             9785..11617
                     /gene="glmS"
                     /locus_tag="ACGI8A_000664"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFH0178676.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            12068..13363
                     /locus_tag="ACGI8A_000665"
     CDS             12068..13363
                     /locus_tag="ACGI8A_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFH0178677.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWREESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            13833..14570
                     /locus_tag="ACGI8A_000666"
     CDS             13833..14570
                     /locus_tag="ACGI8A_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HFH0178678.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            14591..15844
                     /gene="murA"
                     /locus_tag="ACGI8A_000667"
     CDS             14591..15844
                     /gene="murA"
                     /locus_tag="ACGI8A_000667"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFH0178679.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            15931..16995
                     /gene="spoIID"
                     /locus_tag="ACGI8A_000668"
     CDS             15931..16995
                     /gene="spoIID"
                     /locus_tag="ACGI8A_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HFH0178680.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            17061..17729
                     /locus_tag="ACGI8A_000669"
     CDS             17061..17729
                     /locus_tag="ACGI8A_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFH0178681.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            17827..18087
                     /gene="spoIIID"
                     /locus_tag="ACGI8A_000670"
     CDS             17827..18087
                     /gene="spoIIID"
                     /locus_tag="ACGI8A_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HFH0178682.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            18284..19303
                     /locus_tag="ACGI8A_000671"
     CDS             18284..19303
                     /locus_tag="ACGI8A_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HFH0178683.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            19319..19747
                     /gene="fabZ"
                     /locus_tag="ACGI8A_000672"
     CDS             19319..19747
                     /gene="fabZ"
                     /locus_tag="ACGI8A_000672"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFH0178684.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNITVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(19858..20367)
                     /gene="yyaC"
                     /locus_tag="ACGI8A_000673"
     CDS             complement(19858..20367)
                     /gene="yyaC"
                     /locus_tag="ACGI8A_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HFH0178685.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      20556..20659
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            20796..21989
                     /gene="metK"
                     /locus_tag="ACGI8A_000674"
     CDS             20796..21989
                     /gene="metK"
                     /locus_tag="ACGI8A_000674"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFH0178686.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            22304..23401
                     /locus_tag="ACGI8A_000675"
     CDS             22304..23401
                     /locus_tag="ACGI8A_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178687.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            23665..25884
                     /locus_tag="ACGI8A_000676"
     CDS             23665..25884
                     /locus_tag="ACGI8A_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFH0178688.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEENFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            25887..26669
                     /locus_tag="ACGI8A_000677"
     CDS             25887..26669
                     /locus_tag="ACGI8A_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFH0178689.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            27001..28914
                     /locus_tag="ACGI8A_000678"
     CDS             27001..28914
                     /locus_tag="ACGI8A_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0178690.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            29055..29363
                     /locus_tag="ACGI8A_000679"
     CDS             29055..29363
                     /locus_tag="ACGI8A_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFH0178691.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            29387..29713
                     /locus_tag="ACGI8A_000680"
     CDS             29387..29713
                     /locus_tag="ACGI8A_000680"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0178692.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            29752..31086
                     /gene="celB"
                     /locus_tag="ACGI8A_000681"
     CDS             29752..31086
                     /gene="celB"
                     /locus_tag="ACGI8A_000681"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFH0178693.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            31097..31618
                     /locus_tag="ACGI8A_000682"
     CDS             31097..31618
                     /locus_tag="ACGI8A_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdB-related putative oxidoreductase"
                     /protein_id="HFH0178694.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            31711..32670
                     /locus_tag="ACGI8A_000683"
     CDS             31711..32670
                     /locus_tag="ACGI8A_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HFH0178695.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            32719..33771
                     /locus_tag="ACGI8A_000684"
     CDS             32719..33771
                     /locus_tag="ACGI8A_000684"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HFH0178696.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            33774..34448
                     /locus_tag="ACGI8A_000685"
     CDS             33774..34448
                     /locus_tag="ACGI8A_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFH0178697.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            35001..35552
                     /gene="raiA"
                     /locus_tag="ACGI8A_000686"
     CDS             35001..35552
                     /gene="raiA"
                     /locus_tag="ACGI8A_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFH0178698.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQENYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            35684..38359
                     /gene="secA"
                     /locus_tag="ACGI8A_000687"
     CDS             35684..38359
                     /gene="secA"
                     /locus_tag="ACGI8A_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFH0178699.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            38374..39481
                     /gene="prfB"
                     /locus_tag="ACGI8A_000688"
     CDS             join(38374..38448,38450..39481)
                     /gene="prfB"
                     /locus_tag="ACGI8A_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFH0178700.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            39625..41766
                     /locus_tag="ACGI8A_000689"
     CDS             39625..41766
                     /locus_tag="ACGI8A_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFH0178701.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            41914..43080
                     /locus_tag="ACGI8A_000690"
     CDS             41914..43080
                     /locus_tag="ACGI8A_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFH0178702.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      43140..43257
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            43382..43987
                     /locus_tag="ACGI8A_000691"
     CDS             43382..43987
                     /locus_tag="ACGI8A_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFH0178703.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     gene            44316..45356
                     /locus_tag="ACGI8A_000692"
     CDS             44316..45356
                     /locus_tag="ACGI8A_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFH0178704.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            45495..45947
                     /gene="tsaE"
                     /locus_tag="ACGI8A_000693"
     CDS             45495..45947
                     /gene="tsaE"
                     /locus_tag="ACGI8A_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFH0178705.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            45947..46663
                     /gene="tsaB"
                     /locus_tag="ACGI8A_000694"
     CDS             45947..46663
                     /gene="tsaB"
                     /locus_tag="ACGI8A_000694"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFH0178706.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            46653..47129
                     /gene="rimI"
                     /locus_tag="ACGI8A_000695"
     CDS             46653..47129
                     /gene="rimI"
                     /locus_tag="ACGI8A_000695"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFH0178707.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            47135..48151
                     /gene="tsaD"
                     /locus_tag="ACGI8A_000696"
     CDS             47135..48151
                     /gene="tsaD"
                     /locus_tag="ACGI8A_000696"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFH0178708.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            48448..51156
                     /gene="hpdB"
                     /locus_tag="ACGI8A_000697"
     CDS             48448..51156
                     /gene="hpdB"
                     /locus_tag="ACGI8A_000697"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HFH0178709.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            51160..51417
                     /gene="hpdC"
                     /locus_tag="ACGI8A_000698"
     CDS             51160..51417
                     /gene="hpdC"
                     /locus_tag="ACGI8A_000698"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HFH0178710.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            51410..52360
                     /gene="hpdA"
                     /locus_tag="ACGI8A_000699"
     CDS             51410..52360
                     /gene="hpdA"
                     /locus_tag="ACGI8A_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HFH0178711.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            52676..53638
                     /locus_tag="ACGI8A_000700"
     CDS             52676..53638
                     /locus_tag="ACGI8A_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFH0178712.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(53737..>54312)
                     /locus_tag="ACGI8A_000701"
     CDS             complement(53737..>54312)
                     /locus_tag="ACGI8A_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFH0178713.1"
                     /translation="KNLYPVKNIKLGSLLIIYFMISVIVPTSFILILQGAQPNYSGII
                     KFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLGVIILGMCWSLWHLPLIFT
                     LYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAIIHVLNNSAFLITNSKEST
                     ITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT        21897 a         5772 c        10295 g        16348 t
ORIGIN      
        1 agttaataag tggattatag aatttataat aattaatata tattaaacaa agatatatta
       61 agatatttta gaacataaaa ctgaattaag gcgcgagtac tattttgtat tcgcgccttg
      121 atggtttgag ttatttgagt aaaaactaac tttaataaga aatatgtatt tattaagttg
      181 atatttttaa tgtgtttaga gattatttaa caatctccaa atataaaact agaattattt
      241 acataaaagt taaatagttt gtatagagtt agatttatat agtataatac ttagtgtaaa
      301 taattaagaa gttatcaaac aggaggataa aactgtgtta gatataaatt cttttttaaa
      361 tggatttttt aatataatat taagaatgag tatatatgat ttaattgaca tatctatagt
      421 agcgtatatt ttttacaaaa tatttatgtt tataaaagat acaagagcag aacaagtgtt
      481 taaaggtata atatttcttt tattagcaac tcaacttagt aacaccttta aattgcatac
      541 cgtttattgg atatctctta aagctttaga ttatggagtt atagctgctc ttataatttt
      601 tcaaccagaa tttagagcag gacttgaaca tataggaaga gcaaagttta atttatttgg
      661 aaagaatgta aatacttctg aagaaactct caatagaaat atagaagaaa ttgtagaagc
      721 tttatattca ctttctagac aaaaaatagg agctcttatc ataatggaga gagaaactag
      781 aataagtgat ataataaata caggaactat tattgatgct gaaatttcta ggcaactatt
      841 aataaatata tttataccaa atacaccatt acacgatgga gctgttgtaa ttagagattc
      901 taaggtaaaa gcagctgcat gttttttgcc attgacagaa agtaaggatt taagtaaaga
      961 tttgggtact agacatagag caggaatagg ggtaagtgag gtgtctgact gtataacttt
     1021 gattgtatca gaggaaacag gtggcgtatc aattgctaaa gcaggtaagt tatatagaga
     1081 tatttctaga gaaagaatga tgaatatatt acgtagtaat ttaaaaacaa atacagaaac
     1141 aagaagtttt ttcaaaggtg gtatatttaa atgataaata gactaaaaaa taataccaaa
     1201 ataaaaataa tatcattttt aagtgcaatt gcattatgga tgtatgttat ggctgtagtt
     1261 gaccctgaag aaactaaagg gtttgaagat atacctgtat ctataagtaa tatgagtgat
     1321 ttaaaggata aaaatcttgt tatatatcca gacgaaaaat taacagcaga tgtatatata
     1381 aaaggtagtt tatcaaaggt acaaagtgtt aaaaaagaag atatacatgt atatggaacc
     1441 attgaagacc caatacaagg acagaaggaa gtatatttaa aagcaaatat tcctcaaggt
     1501 gttactctag aatttaagca tgatgtttta gtagtaaatc ttgaaaaaaa cataaaagaa
     1561 aaaagagata taaaggtaga agtaagtgga aattcaaaaa gaaatataaa taaaattgaa
     1621 cttagtaagg aatttgtcga tgtatcaggt ccaagaactc tagttaaaga agtcaaaagt
     1681 gtaagagcta atctagatgt tggtaatgag acagatgatt tttctaaaaa attgcaatta
     1741 ataccattaa atagtgatgg aactaaggtt gaaggagtaa cattaagttc atcctttgtg
     1801 actgcaaaga tagaactatt gaaggaaaaa attgtcccaa taagagtaag tctaaatgat
     1861 aataaagata atgatgtact gaaaaactat aaattaagta aggaacaagt tacaataaaa
     1921 ggaaagaaag aagatataga caaaataagt tatattaaga caaaacctat tgacttagct
     1981 gatttggcta atggtgaaga aaaagaagtt agcttagaaa ttccagaggg tataactctt
     2041 gaggatagct ctataacaat taaattaaat gatacaaaac aattgagtgc agaattttta
     2101 tataatgcgg gagatattga attaagaaat atcccaacaa atatggataa tccaaatgta
     2161 atttctgatg tagatataaa agttgttgta gagtcaatgg aggatttgag taagttaaat
     2221 aaaaaagata tttcattata tattgattta tcacaaggtc ctgaggaaga tagtaaatat
     2281 aaaataaaat atgaaacaac atatcaattt aaaaaaataa atatagaacc taatattgtt
     2341 gaagtggaat gaacaaatat ttcgaatatt tagttgttac aagatgcgac aaaaaaacct
     2401 ataaaattaa gtttgttaat tttatgtttt tgttgcatct tgtttatttt agttatatta
     2461 taaaataagg acaatgagat atttgttaaa tctaaaaagc gctagatata tgaaaaaaga
     2521 ctgataaata gatttaaata aatattttga attaaatatt tcaattatct ctaggaaaac
     2581 gttgctgaac gagttataat ttaattgaga tataaaaagt tctatttcaa aaaattcaag
     2641 atgaggtgga aatgcatgag aagttttgaa gaagtaatta agtttgcaaa agaaagagga
     2701 cctaaaacta tatcagtagc atgttgccaa gataaagaag ttttaatggc agttgaaatg
     2761 gctagaaaag aaaaaatagc aaatgccatt ttagtaggag atatagaaaa gactaaagaa
     2821 attgcaaaaa gcatagacat ggatatcgaa aattatgaac tgatagatat aaaagattta
     2881 gcagaagcat ctctaaaatc tgttgaatta gtttcacaag gaaaagccga catggtaatg
     2941 aaaggcttag tagacacatc aataatacta aaagcagttt taaataaaga agtaggtctt
     3001 agaactggaa atgtattaag tcacgtagca gtatttgatg tagagggata tgatagatta
     3061 tttttcgtaa ctgacgcagc tatgaactta gctcctgata caaatactaa aaagcaaatc
     3121 atagaaaatg cttgcacagt agcacattca ttagatataa gtgaaccaaa agttgctgca
     3181 atatgcgcaa aagaaaaagt aaatccaaaa atgaaagata cagttgaagc taaagaacta
     3241 gaagaaatgt atgaaagagg agaaatcaaa ggttgtatgg ttggtggacc ttttgcaatt
     3301 gataatgcag tatctttaga agcagctaaa cataaaggta taaatcatcc tgtagcagga
     3361 agagctgata tattattagc cccagatatt gaaggtggta acatattata taaagctttg
     3421 gtattcttct caaaatcaaa aaatgcagga gttatagttg gggctaaagc accaataata
     3481 ttaacttcta gagcagacag tgaagaaact aaactaaact caatagcttt aggtgtttta
     3541 atggcagcaa aggcataatt ttaaggggga aataggcaaa tgagcaaaat atttaaaatc
     3601 ttaacaataa atcctggttc gacatcaact aaaatagctg tatttgataa tgaggattta
     3661 gtatttgaaa aaactttaag acattcttca gaagaaatag gaaaatatga gaaggtgtct
     3721 gaccaatttg aatttcgtaa acaagtaata gaagaagctc taaaagaagg tggagtaaaa
     3781 acatctgaat tagatgctgt agtaggtaga ggaggacttc ttaaacctat aaaaggtggt
     3841 acttattcag taagtgctgc tatgattgaa gatttaaaag tgggagtttt aggagaacac
     3901 gcttcaaacc taggtggaat aatagcaaaa caaataggtg aagaagtaaa tgttccttca
     3961 tacatagtag accctgttgt tgtagatgaa ttagaagatg ttgctagaat ttctggtatg
     4021 cctgaaataa gtagagcaag tgtagtacat gctttaaatc aaaaggcaat agcaagaaga
     4081 tatgctagag aaataaacaa gaaatatgaa gatataaatc ttatagttgc acatatgggt
     4141 ggaggagttt ctgttggagc tcataaaaat ggtaaaatag tagatgttgc aaacgcatta
     4201 gatggagaag gacctttctc tccagaaaga agtggtggac taccagtagg tgcattagta
     4261 aaaatgtgct ttagtggaaa atatactcaa gatgaaatta aaaagaaaat aaaaggtaat
     4321 ggcggattag ttgcatactt aaacactaat gatgctagag aagttgaaga aagaattgaa
     4381 gctggtgatg aaaaagctaa attagcatat gaagctatgg catatcaaat ctctaaagaa
     4441 ataggagcta gtgctgcagt tcttaaggga gatgtaaaag caatattatt aactggtgga
     4501 atcgcatatt caaaaatgtt tacagaaatg attgcagata gagttaaatt tatagcagat
     4561 gtaaaagttt atccaggtga agatgaaatg attgcattag ctcaaggtgg acttagagtt
     4621 ttaactggtg aagaagaggc tcaagtttat gataactaat gataaaagaa taagaatcat
     4681 aactggtcac tatggaagtg gtaaaagtga atttgcgatg aactatgttg ttaagttaag
     4741 agatatggtt tctggcaaag ttgctatagc cgatttagat gtggtaaatg tatactttag
     4801 aactagagaa aagaaagagt taatgaaatc cttaggtata caaccaatag atagctcaat
     4861 taatgctcca acattagatt taccagcagt atcagcagag gtaatgtctc ctatggtaga
     4921 ccattcatac aattctgtta tagatttagg tggagataat gtaggtgcta gggttatagg
     4981 aagatttagt catttgttaa aagaaggcga ttatgatatg ctttttgtaa taaatgcaaa
     5041 tagggagaaa actcaaactt ctgaagaagt tatccaatat ataaaagaaa tagaaaaatc
     5101 ttctaaatta aaggtaacag gtctaattaa taatactcat cttattaggt ttactactat
     5161 tgatgatgtt ttaagaggtc agaaagttgc aaaagaagtt tctgaaaaat gtaatatacc
     5221 gataagatat gttgcatgtt tagaaaatct tgttgaacag ttaccaaaag atttagaagg
     5281 agaaattttt cctataaaat tatatatgag agaagactgg atgtagtttg agctcaaata
     5341 tatagaaagt ttaaggaggc ttaggtaaat ggctaaagga aaagtatctt ttaatcaaga
     5401 acgttgtaaa ggttgtggat tgtgtgttga agcatgtcca gtaaaaatca tacaattaga
     5461 ctcaaatgta ataaacaaaa aagggtataa cccagcaact gtttttgaaa tggaaaaatg
     5521 cataggatgt gcaagttgtg caactatgtg tccagatgta gttataacag tagaaagaga
     5581 ttaatttaat taatatttaa gggggaattt taatggctaa gatacttatg aaaggtaacg
     5641 aagcatttgg aaaagcagct atagaagctg gttgcaaata tttcttcggt tacccaataa
     5701 ctccacaaag tgaattacca gaatatttat caagagagtt acctaaaata ggtggagcgt
     5761 tcgttcaagc tgaatcagaa gtttctgcga taaacatggt ttatggtgga gcaggtgcag
     5821 gagcaagagt tatgacttct tcatcttcac caggagttgc tttaaagcaa gaaggtataa
     5881 catatgcagt aggagcagaa gttccttgtg ttgtacttaa tgtaatgaga ggtggtccag
     5941 gacttggaag tatacaacct tcacaagctg actactttat gtctacaaga ggtggaggaa
     6001 atggagacta cagaactcca gtattcgcac cagctacagt tcaagaagct gttgatatga
     6061 taatggaagc ttttgacgtt gcagattatt acagatcacc agttatggta gttgcagatg
     6121 gtatgatagg tcaaatgatg gagccagttg aatttagagc tcctgagaag aaaagagaat
     6181 tgcctcctaa agattgggca actgttggaa ctaaaggaaa aagaaaacct aatgtaataa
     6241 actctctata cttagagcca gaagtactag aagaccattg ttggcattta caagaaaaat
     6301 ttgatgcaat ggaaaagaat gaagtacaat acgaaatgta caaaacagaa gatgctgaat
     6361 ttgtatttgc agcttatgga actacttcaa gagtagtaaa aagcgcaatt gatatattaa
     6421 gagaagaagg aataaaagca ggtcttataa gacctaaagt attatggcca ttcccattcg
     6481 aagcattcaa ccaaattcct aatgctagaa acatattgac tgttgaaatg agtatgggac
     6541 aaatggtgga agatgttaaa atggcagtag aaggtaaatt accagtttac ttccatggaa
     6601 gaccaggagg aatgactcca acaccagcag aaatagttga aaaagctaaa aaaattattg
     6661 ccggagaatt ggttgcagga ggtgctagat aatggctgtt gtatttaaga aaactgaagg
     6721 attacaagat actcaaactc actactgtcc aggatgtaca catggtataa tccatagatt
     6781 agttggtgaa gtattagaag aactaggagt attaggtgat gctgtaggtg ttgttccagt
     6841 tggatgttca gttttaggat ataaatattt taactgtgac actcaagaag ctgctcatgg
     6901 tagagcgcca gctgctgcta caggaataaa aagagttcat cctgaaaata cagtattcac
     6961 ttatcaaggt gatggagact tagcttcaat tggtactgct gaaatagttc atgcagctgc
     7021 tagaggagaa aaaataacta ctatatttgt aaacaataca acttatggta tgactggtgg
     7081 acaaatggct ccaactacat tagttggaca aagagctact actgctcaat caggaagaaa
     7141 tgctgaaact caaggttatc caataagagt aagtgaaatg ttagctacat taacaggagc
     7201 tgtatttgta gaaagagtag cagttgatac tccagctcat gttagacaag ctaaaaaagc
     7261 tataaagaaa gcattccaag ttcaacaagc aggattagga tttggaatag tagaagtatt
     7321 atctacttgt cctacaaact ggggactagc tccaaacgat gctttacaat ggttaagaga
     7381 caacatgata ccatactatc cattaggaaa tttcaaaaat gttgaagttg aggaggtgaa
     7441 gtaaatgtct acagctagag taatatgtgc aggcttcgga ggccaaggtg ttatgtctat
     7501 gggacaatta cttacttatg caggaatgct agaaggaaaa gaagtttctt ggttaccatc
     7561 ttatggacca gaaatgcgtg gaggtacagc aaactgtgca gtaactgtat ctaatgagcc
     7621 agttggttct ccacttataa cagatgatgc aactgcagct atattattaa atataccagc
     7681 atttgaaaaa ttcaaagatg atgttattcc tggtggaaaa ataatagtaa atagttcttt
     7741 aataaaagaa aaagttgata gaacagatgt agatgtttat tatataccag caaatgaatt
     7801 agcagctgaa ttaggaaatg ataaagttgc taacatgata atgttaggcg cttacttaaa
     7861 agtatctgat actgtagata tagaatctgt actagaagca ttcaaaaaag ttttcggacc
     7921 tagaaaagaa aaattcgttc cattaaacag agaagcttta caaaaaggta tggatgctac
     7981 atgttctaaa gcaactaact aataaagaaa aagatgtcaa aaaaaagaca atagaattat
     8041 ttctattgtc ttttttgctg ataagtatta aaatattttt atgactgaaa ttaagcctat
     8101 aatgatattt tatgaactat aaaaatataa tacaaattac aagcttttca attatgaaag
     8161 gagaatcttt agtgagaaag tattttggaa cagatggagt tagaggagta gcaaatacag
     8221 aactaacatg tgatttagca tataaacttg gtagagctgg tggatttgtc ttagctcaag
     8281 gagaccacag agtaaaagta gtagtaggta aggatactag aatatcaggt gatatgttgg
     8341 aagcatcttt aatagcagga cttatgtctg ttggatgtga tgttattact gttggaataa
     8401 taccaacacc tgcagtggca tatttaacta gaaaatatgg tgctgattgt ggtgtggtta
     8461 tatctgcatc tcacaatcct gttgaatata atggaatcaa attctttaat aagaatggat
     8521 ataaattaga tgatgaaatt gaattgaaaa tagaagaata tatagacgat atagacaaaa
     8581 ttgattgtct tccaattggt gaaaatgtag gaagaaaatt acatgaacat tgtgcacaaa
     8641 gagactatgt agactaccta aaatcaataa taagcactga ctttaaggga ttaaaagtag
     8701 ttttggactg tgctaatggt gcgtcatata aagtggcacc aatagtgttt gatgaactag
     8761 gagcaagtgt aatttcaata aatagttcac cagatggaaa taatataaac tataaatgtg
     8821 gctctacaca tccagaacaa cttcaaaggg ctgttttgga acataatgct gacttaggtc
     8881 ttgcatatga tggagatgct gatagattaa ttgcagtaaa tgaaaagggt caaattgttg
     8941 atggagacca tataatgata ttgagtgcct taaatttaaa gaaaaataat aaattggcac
     9001 aagatacttt ggtagttaca gttatgagta atatagggtt gacaatagct gctaaggaaa
     9061 atggaataaa tttatctact acagcagttg gagacagata tgttttagaa gatatggtta
     9121 aaaatggata taatcttggt ggagagcaat ctggacatat gatattttta gattacaaca
     9181 ctactggaga tggagtgctt agttctctaa tacttgcaaa tataatcttg caagaaaaga
     9241 aacctttatc agaaattgca tcaataatga gccaataccc acaagtatta gtaaatgcaa
     9301 caataaaaaa tgaaaataag aacaaatata tggaatatcc agaaataaaa actgagatag
     9361 aaagaataga atctatttta gacggaaatg gtagagtttt gataagacca tcaggaactg
     9421 agccattagt tagagttatg ttagagggta aagaggaagg tcaaataaaa gaattagcaa
     9481 caaacttagc taatcttata caagaaaaat tatcgtaatt taaagggtat tctcagatat
     9541 aaatactgag gacacctttc taagcgccag aacttagtta tagacataat tattgagtaa
     9601 tataggaact aagttgacga ggtcagagtt aatcgaattg tcggcggatg ctctgcggtg
     9661 tgtttaccat cgaaagactt ctttaaataa taggagcaat cctatggaca aagggaagcc
     9721 aaagtaaact tatatgacct aactcttaaa atatataaat aatattttta ggaggacaca
     9781 aataatgtgt ggaatagttg gatatttagg aagtagaaag gcagcagaag ttatagtaga
     9841 agggctttct aagctagagt acagaggtta tgactcagca ggagtagcag ttaatagttc
     9901 taatgaaaaa gaacttaata ttagaaagtt taaaggtaga ctttctgtac ttgcagaaga
     9961 cttagaaaag aatcctattg atggaaattt aggtataggg catacgagat gggcaactca
    10021 tggagaacca tctgatgtaa actcacatcc tcattttaat caagccaaaa caatagcagt
    10081 tgttcataat gggataatag aaaactatat ggaaataaaa gaagaattaa tttctgaagg
    10141 tgttaagttt gaatctcaaa cagatacaga ggtaatagca catttagtag ataaatacta
    10201 tgagggtaat ttattagatg ctgtatacaa aactatatca aaattaagag gagcttatgc
    10261 attaggtgtt atatgtaaag aacatggaaa tgaattagtt gcagtaagaa aagatagtcc
    10321 tcttgtagtt ggtgttggtg aaggtgaaaa ctttatagca tcagatatac ctgcattatt
    10381 aaaatatact agagatgttt atttcttaga aaatggagaa gtagttcatc taaaagacga
    10441 aaatgtaaca gtttatgact ctaatagaaa tcttgttgaa aaagaagtat ttcatgtaac
    10501 ttgggatgta gaagctgcgt ctaaaggtgg atatgactac tttatgtcaa aagaaattca
    10561 tgagcaacca acaggagtaa gagaaacttt agaaagaaga cttgatgata atggaaatat
    10621 aattctagac agtataaata tttctaaaga agacttagaa aaaataaata aagtttatat
    10681 agtggcttgt ggaacagcat ataatgctgg attacttggt aagtatgcca tagaaaaatt
    10741 tgtaaatata ccagtaataa ctgatatagc atcagaattt agatatagcg acccatttgt
    10801 agatgaaaat tcattagtaa tattagttag tcaatctggt gagacagcag atactctagc
    10861 agtattaaga gattcaaagg ctaaaggagc aagaatactt tcaataacta atgttgtagg
    10921 ttcatctata gcaagagaat cagatgatgt attctatact tgggcaggac ctgaagtagc
    10981 agttgcatca actaaggcat atactactca aataacttct ttatacatga tagctttaga
    11041 ctttgctatc aaaaaaggaa caataactag agaattttat gatagtatga tttctaagat
    11101 gaaagaaata ccatcaaaaa tacaagaaat actagacaat gaagaatata taaaagaagt
    11161 tgctaaaaca gttgttagtt ctgaacatgc attctattta ggaagaggta tagactatag
    11221 cttagcaatg gaaggttcat tgaaattaaa agagatttca tatatacatg cagaagcttt
    11281 tgcagcagga gagctaaagc atggaacaat agctttaata gaaaaaggaa ctcctgtaat
    11341 tgccatagct actcaagaaa agttatttga aaaaatggtt tctaatatgg aagaagtaag
    11401 agcaagagga gcttatgtag tagcaatagc tcaaagccat aataaggatg ttgagaaagc
    11461 tgctgataaa ataatctata taccaaattc agatgatatt ttatcaccaa tattagcagt
    11521 tgtaccaatg caattacttg cttatcatgt ctctgtacta agaggatgcg atgtagataa
    11581 gccaagaaat ctagctaaat cagtaacagt tgaataaaaa cagattaaaa attaaataga
    11641 taatatttat tgaaaagaac tcatcctgtt atttgtattt tataggatga gttttttaga
    11701 taaatattaa atttgttttg tttttgctat aataaatttt ataaaaagat tgatattaaa
    11761 tcatctgtaa aatgataaat atatagacca taccaggtat agggaacgat aaatatagtt
    11821 catataaaat atagtgagta cttaataaac atatagtcaa tatcagataa aaaaataata
    11881 gcttatgaga aaaattaaaa tacaaggtat tagtaacctt gtgataagat aaagtgagta
    11941 gtttaaaata agaatataag gaactattta taaaagaata taatgaagct aatagagtat
    12001 aagaaaatta ctttatttta gtaaaataat ttcaaataaa aattttttta aattgggagt
    12061 ataaattatg acaaatgatt ttaaaaaaga aaagaaagaa gattattttg ttaatcttaa
    12121 ggcaatatct actgaagttt tacaagaaaa agtgcaagat aacgcttggg ttatagttga
    12181 tactcgctta aatgatgctt ataatggctg gaagttagat ggagtaaaaa gaggtggaca
    12241 tataaaagga gcagtagatt tttctgctaa ttggttaagt gtatactcag atagaaaaga
    12301 tgaagtgtta gaacaagcat tgaaaactaa aagaattgat ttagataaaa atatagtatt
    12361 gtatgatgca aatggaaaag atgctttggt tgtagcagat tatttatcta aaaaaggata
    12421 taaatatcta tataaatatg atattaaaca atgggcagac gacgaaaatc ttccaatgga
    12481 aagatataaa aattatcaaa tgatagttcc tgcttttata ataaaagata tattagatgg
    12541 aaaaattcct gagacatttg aagattcaaa gaatataaaa atgattgaag cgagttggag
    12601 agaagaatcg tatacaaaag ggcatatacc tacaagtgtt catgtaaata ctgatattat
    12661 agaacctcct ccaacatgga tgcttgataa tgatgataac ttaactaagt ttgctttaga
    12721 ctatggatta acaaaggatg atactgtaat agtttcaagt agtacaccta tggcatctta
    12781 tagactagct gttatattga gatatatagg agtaaaagat gtaagagttt tgaatggtgg
    12841 aacaaactca tggttatcag caggatatga attagaattt attagtaatc caaaacattc
    12901 atgtactaat tttggagcag atataccagt taactcacaa ttaattgtta ctacatctga
    12961 attaagacaa aaactaaaag aaaaaaataa gtttatacta gtagataacc gtacttggga
    13021 tgaacatata ggaaaagtat ctggatatac atattatgat aaaaaagggc gtataccagg
    13081 agctttatat ggacattcag gtagtgatag tgtttcacta gaagaatatc gtaatattga
    13141 taatacaatg agaaataaat atgagatttt agaaatgtgg gacaaggaaa atatagatgt
    13201 gaataaacag cttatattta tgtgtggaag tggatggcgt gctgctgaag tattaacata
    13261 tgcaaatgtc ataggagtgg aaaatacctc cctttatagt gatggatgga tgggctggag
    13321 tcttgataat tcaaatttaa ttgaagtggg agaacacaaa taaagttttt gaaagtgcaa
    13381 tagagttgtt taaaacaaag gtgtttttga tttattagga agagtgtgta aattgtgtat
    13441 tgggaagtgt acttaggtat gttcatttgg agctatggaa attgataaaa aagtattatc
    13501 tgtttatttt gttggtaaaa tatgaataga ttatagatta tagattatag attatagatt
    13561 atagattata gattatagat ttaagtatag actaagtaat ttatattaca gaagagaata
    13621 tataagaatt aatgcaagat gtatttgata catataagaa attggcaagt ccaggagaag
    13681 gatagagaaa attgagattg aagagtttga aagagattat tagatgaaaa atacaaaaat
    13741 gtataaaaat acatattgag aaagtatatc tctcttaaat ttgttaataa ttctattata
    13801 cagaaaaaca tatttaaggg ggatttttta ctatgaaaaa agtaaaatta gctataagct
    13861 tttttatatt aatgattata ggaatgacaa tatcttatgc agatacaagt ataaatacta
    13921 gatacaatag attaagtaaa gctcttgaaa tatcagaagc aaattttaaa ttttataatg
    13981 ttaaaattaa ttctgttata aatcataatt tatcaaataa agaatttcat aatattttta
    14041 tagaaataat taacaatcta aagttggata aaaaaggaat aaattggaat caaaaagggt
    14101 ctggcgataa aatacaatta tgtgctacta taaaaggtag cgataaagaa atttctataa
    14161 aaggaataaa taaaaataac aaagaaagtt acatagtagt tgacatattg gataacaaag
    14221 tatataaaca taaagaggtt atttatagta acgtagaaaa tgtgttaaat aaatattcat
    14281 ctcaagtaga tgtgtctgct tgtattgttg gagaatacac aaaaaggtta caaaacaaca
    14341 aatatgatga tattttacaa aaaatattat ataatatgag tgctaagcaa atagatgaag
    14401 ttaaagacgt aaacttttta tctataacag catatagtaa agtattaaat gattatgaat
    14461 tagaatattt aggggaaaaa attaatttaa atataggaat taaatataat gaggatgacg
    14521 gaaaaacact catatatatt gccacaccaa ttataaaatt agattattaa ttatgaggag
    14581 atcaaaaaat atggctaaga taatagtaaa aaagagtaat ccacttaaag gtagtgttaa
    14641 aatagatggt gcaaaaaatg cagttttacc aataatagca gcgactttat tagcaaacgg
    14701 aaaatcaaca ttaaatggag taccaaattt aagagatgtt catgttatat cagatttatt
    14761 aagacatgta ggagcagaag ttgaatataa agaaaataca cttactgttg atgcaagcaa
    14821 tataaaaact tgtgaagctc catatgagct tgtaagaaag atgagagctt cttttttggt
    14881 tatgggacca ttgcttgcta gatttaatag tacgaaaatt tctatgccag gaggatgtgc
    14941 tataggaaca agacctatag atttacattt aaaaggattt aaagctttag gtgcaaagat
    15001 agaaatggac catggttttg ttgaagcagc aacagaaaag ttagtaggaa ataaattata
    15061 tttagatttc ccatctgttg gagcaactga aaacataatg atggcagctt ctttagcaga
    15121 agggactaca ataatagaga atgcagcaga agagccagaa atagtagatt tagcaaactt
    15181 cttgaacgaa atgggagcag atgttaaagg tgcaggtact aatactataa aaataaaagg
    15241 tgttaaagag ttaaaaggag cagaacataa tgtaattcca gacagaatag aagcagcaac
    15301 ttatatggtt gcagcagcta tgacaaaagg tgatataact gttgaaaatg ttttaatgga
    15361 gcatttaaaa ccagttgtag ctaaattaag agaagctggt tgtgaaataa cagaaatgga
    15421 caattctgta agagttgtag gtccaaaagt attaaaacca atagatataa aaactttacc
    15481 acatccagga tttcctacag atgttcaagc acaatttatg gctatgctta ctgtagccaa
    15541 tggaactggt gtagttatag aaactgtttt tgaaaacaga tttatgcatg ttgctgaatt
    15601 taatagaatg ggtgctaata taaaaataga tggaagaagt gcggttgtta atggtgtaga
    15661 tgagctacac ggtgcagcag taaacgctac tgatttaaga gcaggggcag cccttatttt
    15721 atgcggtctt atagcagaag gagaaactca aataggagaa atataccata tccaaagagg
    15781 ttatgttgat atagataaga agataacagc actaggtggt caaatcgaaa tagtagaaga
    15841 ttaataattg gtttatagaa gaaagttata aattaatagg gttgttcata tacttaaata
    15901 caaggtttta aaagctaagg aggagagtat atgaagaacc cattggttgt tttgttagga
    15961 tttgtgactt gctctgtgtt agttccatca cttataactt tagtttctta taaaaatgta
    16021 gaattaactg aaaaaccaga gagcccagta tctataaaca aaactattaa aaaaagtgat
    16081 attgaagaca aaggtaataa agaagaaaaa aatacagtga actatgaaac tgtaaataaa
    16141 aaagcaccaa ttatcaatgt atataatcat ataacaggga aaaccgaaaa gatggacatg
    16201 gagaactatt tatgtggagt actagctggt gagatgtctt cagagtttga tatagaagct
    16261 ttaaaagctc aatctgtagc agctagaaca tatgtagtat ataaacagga acatggtaaa
    16321 tctagtaaac ataaaaatgc agtagtatgt actgattata aacattgtca agaatataag
    16381 agttatgata ctcttaaaaa attaaatggt gaagaatgga ttaagaataa atattccaaa
    16441 atccaagaag cagttagagg aactaaaggt caaataataa cttataatga taaggcgata
    16501 cttcctcttt atttttctac atcttcagga aaaacagaaa atagtgaaga agtattttct
    16561 gcaaaatatc cttatttaaa gtctgtggaa agtccttatg ataagtattc ccctaaattt
    16621 gcgtcaacac ttaaaataag taacacagat tttgttaaaa gtcttagaag ggcatatagc
    16681 actattgtga tagatgtgaa taatttaagt aaacaagttt caataactaa aagaagtgat
    16741 gccggaactg tagaaaaaat aaaattagga aataaagaat tgactggaaa agacattaga
    16801 actgttttta agttgaactc tgcaaacttt gacataaaat ttggagaagg atacatagat
    16861 ttcgtggtaa aaggatatgg tcatggtgtt ggtatgagcc agtggggagc agaaggaatg
    16921 gcagaagagg gttataaata ttatgatata ttatcacatt actacacaga tacaaaaata
    16981 aaagatatat actaataatt gtataaaatg ccaaaaacac gtcaataatc caaatgtaga
    17041 aatatatttg gaggtgcaaa atgaagaaaa agctgttaga aaaagatggt ttttatttat
    17101 ctttgtttgt atgtgtttgt ttattagcag taggtggagt ttggtttaca aataataatg
    17161 tagataaatt ggcttctaat aaaggaatta tggaaaatgc taataaagat agtgaagagg
    17221 aaatacattt aattgaaaaa gacaagaaag atgctatccc tactgcaacg gattcaaaac
    17281 aaaacttaga aaaagctaag tcaaaagagg aaaataaatc gagtacaaca aaattaaatt
    17341 acattggaga taaagtaata agaggatatt cagaaaaaga gcctagttat tctaaaacac
    17401 ttgatgtttg ggaaactcat aaaggtgtag atattagttg tactaaaggc aaagaagtaa
    17461 aatctctatt aaacggaata gtagtagatg tatttgatga tgaagaatat ggacaatcag
    17521 tcaaaataaa aagtgacaat aacattgtag tagtatattc aaatctagat aaaaatgtta
    17581 gtgttaagaa agaacagaag gtaacagaag gacaatctct aggtactgta ggtagtactt
    17641 ctcaaataga aagtgaagaa ggtatacatg tgcatttaga ggcgtatagt ggagaaaaat
    17701 ctatagaccc aatgagtcta attaagtaat agtataacaa gtttaatcat atttggttaa
    17761 gcttgttata ttttttggtg tttttacata tatatcatta aagctattat gtgagggggg
    17821 taatctttga gatctcatat agaggaaagg gccatagtgg tagcaaaata tatacttgaa
    17881 aaaaacacta cagtaagaca aaccgccaag acatttggag taagtaaaag tacaattcat
    17941 aaggatgtta cagaaagatt gaaggaaata aatccatccc ttgccaaaga agttaaaaat
    18001 gtgctggaca aaaataaatc agagagacat attagagggg gattagcaac gaaattaaaa
    18061 tatgagaagg aacataaaaa aatgtaggtt aaaaacctac tttttttatt gaattaaatt
    18121 ataaaaagaa atatattaag taattataaa tagcaaaata ataatactct caaaagattt
    18181 attttctctt gattaaagcg agatttttgt atatgcttta ttataggagt attaaaatag
    18241 gcttataata taatctaata ttataaataa aggagttgaa aaaatggctg gagctgatat
    18301 aggcatagat ttaggtactg caaatgtgct tgtttatgtt agtggtaagg gaatcgtttt
    18361 agaagaacca tctgttgtgg cgatagacaa aagaacagat tctgttttag ctgtgggaga
    18421 agaagctaaa aagatgatag ggagaacacc aggtaatata gtcgctataa gacctttaag
    18481 agatggtgta atttcagatt atgatgtaac tgagaagatg ttaaaatctt ttatagataa
    18541 gatagttgat aaaaagggat ttggaagatt ttttatgcca agaattatgg tatgcgtacc
    18601 aacaggtgtc acagaagtgg aaaaaagagc agtagaagat gctacgagac aagctggagc
    18661 tagagaagta tatatcatag aagaaccaat agcagctgcc ataggagctg gtgtagacat
    18721 atcacagcct aatggtaata tgattataga tattggtgga ggtactgtag atatagctgt
    18781 aatatctcta ggaggagctg ttgtaagtga atctataaag gttggtggag atagatttga
    18841 tgatgctata gttaaatata tgaaaaaaca acacaatctt cttataggag aaagaacagc
    18901 agagaaaatc aagtttgaaa taggttctgc atttaagaga gaagaagaaa aatatatgaa
    18961 gataacagga agaaatttaa ttacaggact tcctagttct ataactataa attcaacaga
    19021 aatgttggaa gcacttagag aatgtgttga gcaaatagta gttgctactc atgctgtttt
    19081 ggaaaaaacg cctcctgagt tagcagcaga tataggagat gtcggtataa taatgactgg
    19141 aggaggttca ttattatatg gattagataa aataatagaa cagaggacag gaataactgt
    19201 tacaatagca gatgaaccat tatcttgtgt tgcaaaaggt acaggaatag ctttaggttc
    19261 aatagactta cttgaaactg gtgggtcttt caaaagaaaa taattaggag agtttagaat
    19321 gttaaatata gatgaaataa aaaaattaat accacataga tatccatttt tattagtaga
    19381 caagattaca gagctagaag ttgggaaaag agctgttggt ataaagaata taacagttaa
    19441 tgaaccattt ttccaaggtc actttccaga atatcctttg atgccaggag tactaatagt
    19501 agaagctcta gcacaagtat gtggagtggc aatgatgtct gtagaggaaa acaaaggaaa
    19561 actaggtgta tttgcaggta tagataaagt tagaatcaag agagaagtaa gaccaggaga
    19621 tacattaact atggaagttg aaatgacaac tcttagaaaa aatatagcta aggcagatgc
    19681 taaggcatat gtaggtgaag aacttgtctg taaaggtgaa ttaatgtttg ctttagtaga
    19741 aaaataaaaa cttgaaatat aaaagtaaaa aggtagataa tttggagtat ttaatcagat
    19801 actctaaaat atctaccttt ttatataaag aattttatca tttagtttat atttagatta
    19861 ggtggatact aataaagcca aggctattgt ttcagctaaa tctaatataa agctaaggcg
    19921 tatattatta aatgaaagtt tattattttc atcaatttta tctacaattc ctactataga
    19981 ataatttcct atttgaggta gttttttacc aacacctttt ccgggcagga taggtccttc
    20041 tctaatctgt atattaccta tgttactctg cgaccccaag caagcatcta tagccaagaa
    20101 gttaccttgt atatgtgtat tttttatagt atctaaagac tcatatatat ttaaggcgtg
    20161 aatagggtta tctagtgtac cgtaaacggg gtatttaaag ttactgttct taagtattgt
    20221 tccaactaaa gggcctaaag tatcacctat agccctgtct gttcctatgc aaacaacaat
    20281 cgtattttca ttaattatag tttttagaac tgaacttagc atctgtataa catcatcatc
    20341 gctgtagttt actttagcta aatacaatag gaacaccccc ttcaaaaaaa tatatgaata
    20401 aaaagcatta aaaagaccta actttaaatt tcagatagat agaaaatttt atttgtctta
    20461 caaaaaaagg aatagaaaaa atatgactaa aaatattgac aaaattttaa aaaaatagta
    20521 agctagtaaa gtaaaacaaa acttaaatct tgattacctt atcaagagag gctgagggat
    20581 aggccctgtg aagcccagca accacccaaa tgggaaaagg tgctaattcc tacaagacaa
    20641 aagtcttgaa agatgaggtc agttaattag tatgtaatta cgattattaa cctcttctta
    20701 taaaggagag gtttttttat tttgagtaaa tttctaaaat taaaaacaag gtaaaaccta
    20761 taataaaaat taagaataaa taagggggaa taaaaatggc aagacattta tttacgtcag
    20821 aatcagttac agaaggacat cctgataaga tatgtgacca aatatcagat tcaatattag
    20881 atgcattatt agaaaaagac ccacaatcta gagtagcttg tgaaactaca gttactactg
    20941 gtttagtttt agtagcagga gaaataagta catctgctta tgtagacatt cctaaattag
    21001 taagagaaac agtaagagaa attggatata caagagcaaa atatggattt gattgtgata
    21061 cttgcgcagt tataacttct attgatgaac aatctggaga tatagctatg ggagttgatg
    21121 agggtctaga aagtaagact ggagaagaga tagaagaaga aatagaaaaa gttggagctg
    21181 gagaccaagg tataatgttt ggatttgcat gtaatgagac tccagaatta atgccacttc
    21241 cgatatcttt ggcacataaa ttatcaagaa gacttacaga agttagaaaa acaggtttag
    21301 ttgattattt aagaccagat ggaaaaactc aagttactgt tgaatatgaa ggaagtaaag
    21361 ctgtaagagt acatacagtt cttatatcag ctcaacattg tgaaacagta tcaaatgata
    21421 aaataagaga agatttaatt aatcatgtta ttaaggaagt tataccagca gaattacttg
    21481 atgaagagac taaaatttat ataaatccaa caggaagatt cgttatagga ggaccacaag
    21541 gagatacagg tcttacagga agaaaaataa taatagatac ttatggtgga tattctagac
    21601 atggtggagg agctttctca ggaaaagacc ctacaaaagt tgatagatct gctgcttacg
    21661 cagctagata tgttgcaaaa aatattgttg cggctggtct tgcagacaaa tgtgagatag
    21721 aacttgcata tgctataggt atagctagac cattatctat atttatagat acttttggga
    21781 caggcaaggt ttcagaagaa aaacttgttg aattagtaaa taaacacttt gatttaagac
    21841 cgggagcgat aataagagac ttagatttaa gaaagcctct ttataaaaag gttgctgctt
    21901 atggtcactt tggtagaact gatatagatt taccttggga aagaactgac aaggttgagc
    21961 agttaagaaa agatgcttta ggtgaataat ttttaactaa aaagctgtta gaatgtaatt
    22021 ttacgattac tttctaacag cttttttatt atgagttaaa tatttttaat aaatgttaat
    22081 ttatcatcat ataatgaata aacagtattt aatctggtca atacatttta tataatactc
    22141 tactaaattt atacatatta tgggatatat gttaaaggat ggttaagaat gtgttaacta
    22201 atgttaaata attaaaaata tattgaaata tcacaataat gagtctaata taatcttaat
    22261 aaacttacac tttaaaaata attttaattg gggggagtag tgtatgtcag gcaaagtaaa
    22321 tattaaaacg gttataagtt ttgctggagc ttatgttgct actgtaatag gttctggatt
    22381 tgcaactggt caagaaatac tgcaattttt tacgttttat ggatatgcgg gtattatagg
    22441 tggaataatc tctatggtac tattttcatg gtttggagca gaagttatag ataagggaag
    22501 agaattgaaa cttaaggagc caataaaaat ttatcaagta tattgtggaa aatatcttgg
    22561 aacatttttt gaatggtttg gtccattgtt tttatttgga acattagtaa taatgattgc
    22621 aggagcagga gctacattat cagagtacta tggattaaat ccatatgtag gtaggattgg
    22681 tatggcaatc gtttccctaa taactgtttc attaggtttg acaaggcttt ctaaaatact
    22741 aggtaatata ggacctataa taataatatt tacactttta gtaggagcaa ttagtttatt
    22801 tagcaatatt gatgctttat caaatgctgg aaatatggtt gatagtttaa atattaaaac
    22861 tgctacatca aatggatatt tctcgggagt actttatact tgttacaatg taataatagt
    22921 aataacattc ttgacaggaa tgggagcttc tgcagtaaat aagaaagatg ctgtatgggg
    22981 tggtatagta ggtggagtag cacttatggc agcagctata atgatgaatt tggcattact
    23041 ttcagacata ggaaatatat acacaaaaga gataccagca ctttacttag ctgataagat
    23101 atctccaata ataggtattt tattctcagt agtactttta ctaggaatat acactacagc
    23161 tgtaccattg ttatggtcag taacaaaccg atttgtagaa gatgaccacc ctaagtttaa
    23221 aattataaca atagtggtaa gtatattagc ttgtatagga ggtttactgc catttgataa
    23281 gttagtaggt acactatatc catatacagg ttacatggga attttaattt tactatgtat
    23341 gctttataga agaattacta aaactgaggg gtacaaggag aataaatctg aaataagtta
    23401 gtactgtaat atacttatga aatttaattt atactctaaa tatagagtaa ttaaaataaa
    23461 atagctattt aaatatggta cctgtatttc atggtaccat attttatgga tgcagatttt
    23521 ttttgtttct gataaaatgt ataatagttg gaaattacgt taagtataaa aatataaaac
    23581 ttttctacga ttatcatagt tttaatatat aaaattatac taacatttaa atggagcgat
    23641 tattagtatt tttaggagat aaaaatggaa aaattagaag gaatgataag tgaaatagtc
    23701 tttaaaaacg aagataatgg ttatacaata gctcacctgg taaatgaaaa tgatgaaatt
    23761 gtggttgtag gatgtatgcc tactttagca attggagaaa gcatagaagt tgaaggaaag
    23821 tgggtaaatc ataagattta tgggactcaa tttgaagtga atagttttat gccagtaaca
    23881 ccatcatcct tagaaggaat ttatgtttac ttatcttctg gaatgataca tggtattggt
    23941 gaaaaaatgg caaaaagaat aattgataag tttggtgtag atacgttaga ggtgatacaa
    24001 aattcaccag aaaaactaca agaagtagaa ggtataggga gtaaaaaagt aaaacaaata
    24061 gtaaaaagtt atgaagaaga cagagaattg agaaatataa taatacagct atctcctttt
    24121 ggaataacac caaattactg cctaaaaata tataaaaaat ataaaagcag tgcaatagaa
    24181 gttataaata aaaacccata ccaacttgct gaagatataa gaggaattgg atttaaagta
    24241 gctgatagta tagcaagtaa aattggtata gataaaaact ctaaagatag aatttgtcag
    24301 ggaatcttat acactttaaa taaatcttta agtaatgggc atacatattt accagagcat
    24361 gttctaatac aagattcgga aaaattattg gagctaaatg gagaaataat aaaagagtgt
    24421 gtaatgatgc tagtttataa tcaaaaaata catatagaga aagtaaacaa tgaaaatctt
    24481 atatacctta tgccatacta tttagctgaa aatggagtat gtagtcaaat agtaaaatta
    24541 tcacagtatg aatttgaaga cttaaaaata gatatagata acgagattaa tgttttagaa
    24601 gaagataaga aaattaagtt agcagaaaaa caaattttag cagtcaaaga atctgttaat
    24661 agtggagtat taattataac tggtggacca ggaactggta aaacaactac cattaatgct
    24721 atcatagata tatttgaaaa taatggtaaa agtgtgactc tggcagctcc aacgggaaga
    24781 gcagcaaaga gaatgagtga aacttctaat aaagaagcaa aaactataca taggttgcta
    24841 gaaatgggat tttcaacaga tgatgattta acttttttta aggatgaaga agacccaata
    24901 aattcagatg taataattgt tgatgaggta tcaatggtag acattatctt aatgtacaat
    24961 ttacttagag ctataaagtt aggaacaaga gtaattttag ttggagatag tgaccaatta
    25021 ccttctgtag gtgctggaaa tgtacttaag gatatgatta attctaatat cataaatgta
    25081 gttaaattaa atgaaatatt tagacaagcg caagaaagta tgataattgt aaatgcccat
    25141 aagataaaca atggagaacc actttatcta aatacaaaag gtaaagattt cttttttata
    25201 agaaaatcaa ctaatgaaga aattttaaat gaaataatag gattggttaa tgaaagatta
    25261 cctaaatttt ataaagttga taaattaaaa gatatacaag ttttatcttc aatgagaaaa
    25321 ggtgaattgg gagttactaa tctaaatata gagttacaga agtatcttaa caaaaaagaa
    25381 aaatttaaag ttgaagaaaa tttttctaaa agattattta gagttggcga taaggtaatg
    25441 caagtaaaaa ataattatac aaaaaaatgg gaaacagaag accaaaaaga aagtggagaa
    25501 ggtatctata atggagatat aggatatgtt tatcacatag ataaagataa gaaaactatt
    25561 tatatattat ttgaccagac taaaattgta tcatacctat atgatgaatt agatgaaatt
    25621 gatcatagtt tttgtacaac aatacataaa agccagggaa gtgaatttcc agttgtagta
    25681 ttgccaatag cgtgggctcc tcctatgtta cttagccgta atttattata tacagcagta
    25741 acaagagcaa aaaaattagt tgtattagtt ggagatgtga aatatcttga atatatgata
    25801 aaaaataata gagttaatca aaggtattca aatttaggat ataagctaaa taaatttaaa
    25861 caagaagggt tgttgataga ataaatatga aaatatgcaa tgataatttt attaagaatt
    25921 ttaaaaatat gattaataaa tgtttggatt ttatatatcc tgagaatata agttgcataa
    25981 tttgtgataa atccataaag aaaacaaata cttattcaat atgtaaaagt tgttttaaag
    26041 aaatgaattt tatacaagat ggttgcatga agtgtggaaa accaataata cgtcattcaa
    26101 ttgaaaaaga atttattgaa gagtgtagtt attgttttaa taaagacttt tattttgata
    26161 aatcaatatc ttgtattgaa tacaatgatg tgacaaaaaa gatggtgtta gggctgaagt
    26221 ataaccaaaa aacctttatg gctaagtata tagctcaaat aatgaaagaa aaattatatt
    26281 tagaaaacat aaaatttgat tatatattat ttgttccact tcataaaaaa aggctaaata
    26341 aaagaggttt taatcaggca caaaaaatag catttaattt aagtaaaata gtaaatattc
    26401 cattattaga ttgcataagt agaaaaaaat atacaagaat gctatataaa ttgaataaga
    26461 aagaacgaaa agaagaatta aaaaatgtat ttgttgttaa agaaaatgtt aaattaataa
    26521 ataataaaaa tatactttta atagatgata tatttactac tggtctaaca acaaacgaaa
    26581 tttctaaact tttaaagtta tcaggtgcta ataaggtttt tgtattgact ctacttacaa
    26641 aagcaaatga taattatatt atggaataat cttatatatt gacaatttta gtttaaaata
    26701 ttaaaatgaa taagtgatat ttaggtctgt ggttgaaagt ccaagccagt cgcaggcaaa
    26761 gggatccacg taagttaaca ctaaaaattg ggttaatgat catggtgcgg cttagaagta
    26821 agacctaccg ataataaaat cgagagagag tagtagtgcg gcgaacccaa gcgaaccctc
    26881 cagtaggcga gtgtggggtc aaaaaccagg tcagctaaat atcatattaa aagagagcta
    26941 gcataccttt atgtatagct cttttttata tataataaat ttaggttgag tcataaagag
    27001 gtgatatgtt tgaataaaag acaagaaaat ttattaacaa cattgctatt aaaagaagag
    27061 tttataaatg ttaataagat tgccaatgat tttgagtgtt cagaaaggac aataagaaat
    27121 gattgcaaat atattgatga atggttatct actttttgta atgcttatat caaaagaaaa
    27181 cctaatattg ggattaaatt tagtggagat ggaacagaca aaatactggt aaatcagaag
    27241 ttgcgaggtg aagttaaaag agtaaatagt gagcttcata agcaagttga gatactaaat
    27301 ttactacttt gtaataataa aaaagttact ttaaatgatt tatctaataa gttgtacata
    27361 aataaaaata ttgtcagaga agaaataagt aaattatcag tagcattaaa taattataat
    27421 ttaaatatct cgactaaaaa aggcagtggt atatatattg aaggggatga gaaagacata
    27481 agaataatgt tagtatcatt cttatttaaa tatatagata aatacagatt aaacatagat
    27541 gaaatagagt acttccatat agtagatata ataattgtaa aaaacataat gcttactata
    27601 gaagaggata tggatataag acttacagat atatcattca agcaattgat gctatttttg
    27661 cttataaata taatacgaat aaggctggga aattctttaa aagtatcaaa tactaagaaa
    27721 gagcttaagc taaaaatgct tatattggat atagaagatg aattgaaatc taatttatcc
    27781 atagtattaa attatgaaga aaaattgttt atagaatctt taatattggg tatgaacaaa
    27841 caaataaata gtaataatat taagaaatct ttatatctaa atgaagaatt agcaagttat
    27901 acaaaaaaaa ttataaattt agtatcagaa gaatcaggaa taaattttaa taatgatata
    27961 ttactttatg aacaattaat atgtcatttg aatgttacaa tgcatcaaat gaaaagcaat
    28021 gtttacttag aaaatccact tttagataac ataaaaacta aattttgttt tttatttaca
    28081 gtaatatcaa gttcaataga aattgaatta aaaggaaaac aaataacgga agatgaaatt
    28141 ggttatctta cacttcactt tcaaacctct ctagaaagaa aatacagcaa aagggaatca
    28201 aataaaaagg caagtattgt atgtccattt agttttggag taagtatgct attaaaagta
    28261 aagatagaaa agagatttga taacataaaa attattgaaa cattaagaga ggaagattta
    28321 aaaagagata actttaataa gactatagat tttataattg catttcaaga gtatgaaaat
    28381 atccaaaaac caatttttat aacaacacca ttatttactg atgaggatga gaataaactt
    28441 aaagagttca caaacaaaat aaaagaaaaa gacacttcat ataaacttat gaacagatta
    28501 atgatgtctg attatttaat aagagagtta gaacatgatg atatttatga agctataaca
    28561 tatttaacta atttattaat tgaaaaacat tatgcagaaa aagagtattt acaatctatt
    28621 ataacaagag aaaagtcata tccaaccaat gttggaaaag gaatattatt ccctcatgga
    28681 gacatgaaat atataaaaca atctgtttta tgttttgcaa ggttaaaaac accaataaaa
    28741 attagaaatg gaaaggatat aaagattata cttttgttag cgtataaaaa agatgatgat
    28801 agaaaagtat ttagagaatt atttaaagaa atatctaatt taacagagga tgaaaatatg
    28861 ctagatattc taagtaaatg tgacattgaa aaggtaaaag atattcttat ataaaattca
    28921 tagtgaacta tggataagca caaaattttc catgtgatgg aaaaagttgt gcatgttttt
    28981 ttgtttttat aagatattat atgtttgtaa ggtataaata atctaaaagt agagaagcaa
    29041 cttaggagga aaaaatggaa gaaatagttt taaagattat aattcatgct ggaaatgcaa
    29101 aatctatgct ttatgaagca cttgactatg caaaggaaaa cgattttaaa aaagcagatg
    29161 aattaattga aaatgcaaat gaagaaattt taaaagcaca taaagtacaa actgaactta
    29221 tacaaaagga agctggggga gataaatcag atatatctat tttattgatt cactctcaag
    29281 accatttaat gacttgtatg agtgagagaa accttataaa agaaatgatt atgttaagaa
    29341 aagaaattca aaaattaaaa taaaaaaatt aagggggatt taaaaaatga aaagaaaagt
    29401 atatttattt tgtagctttg gtatgtcaac aagtttactt gcagataaaa tgcagaaggt
    29461 agcagatgaa cacaatttac caatagaagt agaggcattt ccgatagcag aaattgacaa
    29521 aatagttgaa gaaaaaaatc cagattgtat attattagga ccacaagtta aatacatgct
    29581 aaaggagtta aaaccaaagt ttgaagctca aggaaaactt atagatataa tcaatgaagt
    29641 tgattatgga actatgaatg gggaaaaagt attaaaactt gctataaagc taataaaatc
    29701 taaacaagcg tagaattata gtaaaaaata ataggaaatg agggtggata gatgaataga
    29761 ttggaaagaa tacttatgcc attggctgaa aaaataggta aaaataaatt attaattgca
    29821 attagagatg gttttttagt atcatcacca ttattaataa taggttcatt atttttatta
    29881 gtagcaaatt ttcctataaa aggatggaat gaatttgtag gtcaaatttt tggtccagat
    29941 tgggctgtaa aattgcaaca accaactgta gcaacatttg aagtaatgac tctacttgca
    30001 gttttaggga ttggatattc ttatgcaaag caaatagatg tagacccaat agcatcagca
    30061 gcagttgcaa tagttgcctt ttttataata acaccatttg taataccata tacaccagaa
    30121 ggaacagaat cagtttacat ggtaacaggt ataccactag gttggatggg ttctaaggga
    30181 atgtttgtag gtatgataac agcaataacc tctgtaaaat tatttgctgc agttgtaaaa
    30241 aaaggatgga cattgaaaat gccagatgga gttcctccaa ctgtatcaaa atcatttgca
    30301 gcattaatac caagtgcaat agttatggta gttttctttt tagttaagat agcttttgaa
    30361 gctacaccat atggaagtat acatgaattt atatttaagt ttttacaaat gccattatta
    30421 aaattaggaa atagtttagg tgctatggca atcgcttata tattcttaca tttattctgg
    30481 ttctttggaa taaatggaag tagtgttgtt ggggctgtgt ataatccaat attaaaaatt
    30541 ctatcagctg aaaacttagc tgcttttcaa gctggttcaa aaataccaaa cataataaca
    30601 ggtcaattcc aagatatgtt tgcgacattt ggtggagcag gtagtacact atcattagtc
    30661 atagctatgt tattaatatg taaaagtaaa cgtataaagt caatgggaaa actatcaatt
    30721 ttaccaggaa tatttggtat aaatgagcca ttgatatttg gtctaccaat aatgcttaac
    30781 ccaatactat tgataccatt tgcaatagtt ccaacaataa atataataat agcttacttc
    30841 tgtatgtcag caggattagt gcctcttaca aatggagttc agttaccgtg gacaactcca
    30901 attatattct ctggtttttt aacaacagga tggcaaggtg cagtcttaca attagtactt
    30961 cttatactag gaatatttat gtatatacca tttataaaaa tgctagataa gcaatacttg
    31021 agagaagaaa atgaaactgt tgaagaggaa gatgatgata tatcttttga tgatttagta
    31081 ttataggagg aagattatga agaaaatagt tatgatattg gaccaaatac aagctggagc
    31141 tggaggaaaa gagaaaagta atatacctcc tgctggaaaa tcaagcccat taggaccagg
    31201 tgtaatgatg gagcaatttt taaatgaatc taaggtaata gctaccttat tttgtgggga
    31261 tgaatttttt gtaaataatc aagaagaagt aacgagtaag atgatagcta tggtaaaaaa
    31321 attaaaccct gatgtagtaa tctgtggacc aagctttaat tacgaaaact tttctaagat
    31381 gtcagcaata ctgtctaaaa atataaatga taaaacagat ataccagctt ttgcagctat
    31441 gtcagaagaa aatatagatg tgataaacga atataaaaat gacatatgta tagttaagac
    31501 tccaaaaaaa ggtggaattg gtcttaatga ttcattaaat aacatatgta aattggcaaa
    31561 agcaatagca aataaagaag atataacttt gatgaaagaa gagttttgtt atcaataaga
    31621 gtattaatta ttagcaagtt taaatattat actatttata ataagcatac attagtaaaa
    31681 tctatataat ttaaggaggg gaattttaac atgtggggaa tgatagcgac atggagaatg
    31741 gccctagaag gaataactga ggcatcagaa catttagaaa aaggcatgga agcaggtgat
    31801 gctgtagagc ttgctgtaag aagagttgaa gactatccat tctataaatc ggttggatat
    31861 ggtggacttc caaatgaaaa ttgtgaggta gaacttgacg ctgcatttat ggatggtaac
    31921 acattagata ttggcgcagt tgctggtata agagattatt ctaatcctgt cagcatagct
    31981 aggaagttaa gttatgagaa ggtaaataat tttttagttg gaataggtgc tgaagattat
    32041 gctcataaaa atggatttga aagaaaaaat atgttgactg atagagcgaa gttacattat
    32101 aaaaagagaa aaaaagagac tttagacaaa ggattaagcc catatgctgg tcatgatact
    32161 gttggtatga tatctttaga caaaactgga aaaatgtgtg cagctacatc aacaagtgga
    32221 ttgtttatga agaaaagagg tagaatagga gattctgcac tatctggttc aggattctat
    32281 gttgatagta gtataggagg agctacagct actggtttgg gagaagattt aatgaaggga
    32341 tgtatatctt atgagatagt tagacttatg aaagaaggga agcatccaca agaagcatgt
    32401 gatattgcag taaatgaact tgataaacag cttatcaaaa gaagaggaaa agctggtgat
    32461 ttatcattag ttgccatgaa taataaaggt gaatggggag tagctacaaa tatagctgat
    32521 tttacttttt cagtagtaac taaagaagaa tctcctactg tatatatttc aaatagagta
    32581 gatggaaaga ctatacataa ggttatatca aatgagtgga tggaagcata tgaggctaga
    32641 ataaaagcac caatagaagt aagtgagtaa agtagtggtt acataattaa aatagagttt
    32701 tataataagg agatttttat gaatatcata agtaataaaa tagatgagtt aaaagaagat
    32761 ttattaagtg atattataga tattgtaaaa atacctagtg tcaaagggga gtcagaaaat
    32821 ggcttccctt ttggcgaaaa agtaggggag gcacttaata aagcattaga aatatcagaa
    32881 aaattgggat ttaaggttag aaacttggat aattatattg gttatgcaga gcatggagat
    32941 agtgatgatt atgtttgtgt aattggacat gtagatgttg tacatgaagg agatggttgg
    33001 aagcatcagc cttataaggg agaagaaact aatggaagaa tttatggacg aggagtacta
    33061 gacaataaag gacctataat gtctgcctta tatggattat atgctattaa agaattaaac
    33121 ttaaaattag ataagagagt tagaataata tttggtacaa atgaagaaag tggatttgaa
    33181 gatataccat attatttaga aaaagaaaaa gcacctatta tgggatttac accagattgt
    33241 aaatatcctg ttgtatatgg tgaaaaaggc atggcaaaga ttagaataaa aagtaaaatc
    33301 aattatgaag aagatgtata cctaggtttt atagaaaata tgtctgaaaa tgtgcttgtt
    33361 acatataaag agctgaatat agaaaatagt gatactatat tagatataaa ggtaaaatac
    33421 gattttagtt ataaattgaa agatgtattg gatgaaataa aagctagttt tccaaatagt
    33481 atcgatatag aggtaatttc taattttaat cctgtatatt ttgataaaga gagtaatctt
    33541 gttaaaaagc ttcagctagc ttatgaaagg gttacatcat tagatggaac tcccgtaaca
    33601 actaatggag gtacgtatgc aaaagtaatg ccaaatatag ttccgtttgg accttcattt
    33661 ccaggacaaa aaggaatagc tcataatcct gatgaataca tggatataga agatataata
    33721 ttaaatgcaa agatatttgc aaatgcaata tatgaattag caaaggagta gttatgttag
    33781 aaataatagg aatgtcagta gaagacgcta aaattataga agattgtggt gcagatagaa
    33841 tagagttagt tagtgcttta actgaaggtg gattgacacc aagttttggt cttatagaaa
    33901 gtgttgttaa cagtgtaaag atacctgtga acgtaatgat aagacatcat gcgaaaagtt
    33961 ttgtatacag taaagaagat ataagtataa tgcaaaaaga tataagtgtg gttaaagaga
    34021 ttggtgctaa tggagttgta tttggtgtat tggataaaaa caatagtata gatgagaaaa
    34081 atctaaatgt tttattgaaa tgttgtgata atcttgatgt tacatttcat agagcaatag
    34141 atgaatcaaa tacaatagat agtgttaaaa ttttgaagga ttatgataaa attacgaata
    34201 tattaacatc aggagggaaa ggaagtatag ttgataacat acaaatgata aagaatatga
    34261 tgcttagttc taatcacata aaaattcttt taggtggagg tcttaatttt aataatatag
    34321 aaaagataaa ggaattaact aaggcaagta attttcattt tggtacagcg attagaataa
    34381 ataacagtcc ttttgaagat atagatagac aaaagcttaa acaactcgta aatataattt
    34441 caagatagta tatgaatata aaatacttag tttaaataaa gagagtaagt taattataat
    34501 ttgggtatat gaatgttaat aggtattaaa tatgaaatta ttgttgagta tgctttataa
    34561 cttttaatta gttttaaata taaataatga aagaaaagat agattatgga ggttttagag
    34621 ggtttatatt tattaaaaaa ttataaaggt atattggcat ataatacaca taatttccta
    34681 aaattaccat cttatgacat gaattcacat attaataaac aattaatgga gatttttgtg
    34741 gatagaaata ggagtaaaaa tagctttaaa taaacaaaaa caagagttat taacagtttt
    34801 atccacatta tccacaggtg tttgtaataa tattaatcca cataagtatc aatgtaacta
    34861 aaaatgataa ataattctat caattgaata gaaaaaatca taaaagtaaa taataaaaag
    34921 aggattatga gagttcgtgt agaatatatt attaaagtat aaattttctt atactggcat
    34981 aattgaaggg agatgtattt atgaacataa ttatatctgg aaaacaaatc gaattaactg
    35041 atgcaataaa aaataaaatc gaaactaaac taagtaagtt agataattac attcatccag
    35101 acactgatgt aaaagttaca gtaagtgcga gaaaagcaag acaaaaaata gaagtaacaa
    35161 tagctactgt aaatggtcct ataattagag cagaagatat gcaggaaaat ttatacacag
    35221 ctatagatgt tgtatatgat aaattaagta aacaattaaa aaaatataaa aagagattac
    35281 aagataaaca taaagacaac aagagtataa gattccaaga tgcagagata aatctagctg
    35341 atgaacaaga gaattatggg gatgaaaatc aagaattagt aattcaaaga cgtaagaaat
    35401 ttagtgttaa gccaatgagt gaagaagaag ctgtattaca gatggaatta ataggacatg
    35461 atttttatat gttcaagaac atagattcag atgaaatagc agtagtttat aagcgtaata
    35521 atggtggata tggtataata gaacatgaat aaagcaattt agatttaatc atattagcta
    35581 tcgataaata aattatcgat agctttttaa attaatatta aaagtatagt ttttatggta
    35641 aaatgaataa tagtacaata aagaacgagg agagatgtaa aatatgagct ttatggataa
    35701 tttattcaac atggcagata aaaaagaatt aaaaaagttc aataaaactg ttgatataat
    35761 agattcatta gaacctaaat ttgaatctat ggcagactcg gaacttaaaa atatgacaaa
    35821 tatatttaaa gaaagattag caaatggtga aagtatagat gatatacttc cagaagcttt
    35881 tgcagtagtt agagaagttt ctaagagagt gttaggactt agacactaca gagttcaaat
    35941 gattggaggt atagtactac atcaaggaag aatagcagaa atgaaaacag gtgaaggtaa
    36001 aactttggtt gcaactgctc ctgtttattt gaatgcgctt actggaaaag gtgtacatgt
    36061 tgtaacagta aatgattacc ttgcaaaacg tgatagagac caaatggcaa aaatctatga
    36121 gtttttagga atgagtgtag gcgttattat acacggtcaa aatccaaaag ttagaaaaga
    36181 acaatatgat tgtgatataa cttatggtac aaataatgaa tatggatttg actatctaaa
    36241 ggacaatatg gtaatacaca aagagcaaag agttcaaaga ggattaaatt atgctatagt
    36301 ggatgaggtt gactctatac taatagatga ggctagaaca ccacttatca tatctggacc
    36361 tggagacaaa tcaactcact tatattcaga tgcaaacaca tttgttttaa ccttaaaacc
    36421 agatgattat gaattagaag aaaaagataa ggctgtatct ctaacagcat cgggtataca
    36481 aaaagcagaa gtgtatttta atgttgataa tataacagat atatctcata cagaattata
    36541 ccatcatata aatcaagctt taagagcaca tgtaataatg aagaaagatg ttgattatgt
    36601 tgctaaagat ggagaaatag taatagtaga tgagttcaca ggtagactta tgtttggtag
    36661 aagatattca gaaggtttac atcaagcaat agaggctaaa gaaggtctta aaatacaaag
    36721 agaatctaaa acactagcta ctgtaacatt ccaaaattac tttagaatgt acaaaaaact
    36781 ttctgggatg actggtacag caaaaacaga agaagaagag tttaaagcaa tctacaaaat
    36841 ggatgttttc caagtaccta ctaataagct tatgataagg gaagacttac cagactgtgt
    36901 atacaaaagt gaaataggta aatttaatgc tgtagcacaa gaaataattg aaaggcacaa
    36961 agtcaatcaa ccaatacttg taggtacagt atcaatagaa aaatcagaat tattatcaca
    37021 aatactaaag aaaaagggca taaagcacga ggtattaaat gctaagcatc atgataaaga
    37081 ggcagaaata atagcacaag ctggtagact tggagctgtt acaatagcta ctaatatggc
    37141 tggtcgtggt acagatatag ttctaggtgg aaatccagac tttttaacta agagagaaat
    37201 gagaagaaat ggatttaaag aagaaatagt aaatagagta gatactccaa tagaaggtat
    37261 acctgtaaaa ggtaatgaaa ttctatttga agctagagaa gagtatgaaa aactatttga
    37321 aaagtttaag caacaaacac aagaagaaca aaaacaagtt gtagaagcag gtggacttgc
    37381 tataataggt acagaaagac acgaatctcg aagaatagat aaccagttga gaggtcgtgc
    37441 tggacgtcaa ggtgacccag ggagttcaag attctatata ggtcttgatg atgaccttat
    37501 gagattgttt ggtagtgata gaatatctgg tattgtagat aaaataggtc ttgaagaaga
    37561 catgccgata gaacatagaa tattatctaa gtctattgaa ggagcacaaa agaaagtcga
    37621 aggtaaaaac tttggaataa gaaagcatgt acttcaatat gatgatgtta tgaataaaca
    37681 aagagaaata atatatgcag agagaaaacg tgtattagaa ggtgaagatt tacaagaaca
    37741 aatacaatca atgactcatt ctataataga agaagcagtt actctttata cacaagataa
    37801 aggatttgat gaagaaggat tcaaagaaca tatgtataat ctgttcttgc caaaaggtag
    37861 catagaaata ccagaaatag aaaaacttaa tccagttgaa ataacagaaa aagtttatga
    37921 aatagctatg aagatatata cttcaaaaga agaacaagtt ggctatgaga gaatgagaga
    37981 agttgagaga gtaatcttac ttcaagctgt tgataaccat tggatagacc atatagatgc
    38041 tatggaccaa ttacgccaag gtattggtct tcgtgctgta ggtcagcaag acccagttat
    38101 tgcttataaa atggaaggtt ttgatatgtt tgatgaaatg aataaacata tcaaagaaga
    38161 tactgtaaga tatttattta atataacaat agaaactcca gtggaaagaa aagcagttgt
    38221 agatgttgaa aatctatctt ctccatcaga tggaacactt ccaacaagta aaactgttaa
    38281 aaaagatgaa aaggttggaa gaaatgactt atgcccttgt ggaagtggta aaaaatataa
    38341 aaactgttgt ggaagataat ctcggaggat taaatgttaa agttacaaga gtatagacat
    38401 agtatggagc agatggcttc aaatttagaa gaattgaggg tttctctttg acatagcttc
    38461 attgatgaaa aatatacaaa ttaatgaaga aaagatgaat caacaagatt tttggaatga
    38521 taatgaagtc gctcaaagag tacttcaaga aaataaatct ttaaaagaga cattagaaga
    38581 atatgaaagc ttaaaaagct tattagaaga tatagaagta ttaattgaaa taggtttaga
    38641 agaagatgat gattctgtag aaagagatat agaaaagtcc atagaatcaa tggaagaaaa
    38701 attatctgaa atgaaaataa agactcttct aaatggagag tatgataaaa ataatgctat
    38761 attatctata aatgctggta ctggtggctt agatgctcaa gattgggctc aaatgctttt
    38821 gagaatgtat ataagatggt cagaaagtaa gggttataaa gttaagcttt tagatataat
    38881 atcagaccct gaagcaggga taaagacagc tacaattctt gtagaaggaa caaatgctta
    38941 tgggtattta aagagtgaaa aaggagttca tagattagtg agaatatctc catttgaccc
    39001 atcaggaaaa agacatactt catttgcatc tatagatgta acaccagagt tagatgaaaa
    39061 tatagaagta gaaattaatc catcagattt aaagatagac acatatagag cttctggagc
    39121 tggagggcaa catgtaaata cgactgattc agcagttaga ataacacata tccctactgg
    39181 tgtagtagtt cagtgccaaa atgaaaggtc tcagcatcta aataaggaca gagctatgag
    39241 acttttaatg gcgaaattga tagaattaaa agagttagag caaaaggaaa aaatagaaga
    39301 tatacaaggc aaatactcac aaataacttg gggtagtcaa attaggtcat atgtttttca
    39361 accatataaa ttggttaaag accatagaac aaatgctgag tttggaaatg tagatagtgt
    39421 aatgaatgga aatatagact tgtttattaa tgaatactta aaaatgaata aaatagttta
    39481 ataaatatgc actgtaatta agatatatgt ataagaaggt tagtgcttaa tatgcactta
    39541 cagcacttag gctgaggacg ataaccctca gccttcgttc tgttttgtta tacttatgaa
    39601 aaactaacat taggagtgaa taaattggat ataaatcaaa tcttaaaaaa agagtttaat
    39661 cttagagatg aacaaataaa taatacatta aaacttattg atgaaggaaa tacaatacca
    39721 tttatagcta gatatagaaa agaaatgact ggcgaaatga gtgatgttac actaagagaa
    39781 ttttatgaaa aattaatgta tttgagaaat cttcaaagta gaaaagatga tgtagttaga
    39841 ctaatagatg aacaagggaa attaacagat gaaataacac aaaatataga aaaagcaaaa
    39901 acattacaag aagtagaaga tatatatgct ccatataaac aaaagaaaag aactagagca
    39961 acaatagcaa aagaaaaagg attagaaaat ttagcattgt caatactaga gaataattta
    40021 gataatatag aaatagaggc aaagaattat cttgatgaag agaaagaagt tctttctatt
    40081 gaagatgctc taaaaggtgc tagagatata attgcagaac tagtctcaga tgatgctaag
    40141 attagaaagt atataagaga attagcttta agagaaggta tgatagttag caaaagtgct
    40201 actgatgaga aatcggttta tgatatgtat tatgattata gcgaagcagt aaaaagtatg
    40261 gctccacata gagttttggc aataaacaga ggagaaaaag aaagcttcct aaaggttaag
    40321 ttggaaataa ataatgataa agttttaaat tacataataa acgaatatgt aaatgataaa
    40381 aatttcaaaa ataaagaaga aatcgtaagt tcaatagaag attcttataa aagattaatt
    40441 ttcccatcaa tagaaagaga aataagaaat catttaacag aaatagctca agaaagagct
    40501 ataagtgtat ttggaaagaa tgttaaaagt ttattgcttc aaccaccagt taaagacaaa
    40561 gtagttatgg gatttgaccc tgcatttaga actggatgta aaatagctgt agtagataaa
    40621 aatggtaaac tactagatta tacaacagta tatccaacag accctcaaaa tgatgtagag
    40681 ggagcaaaga aagttttaaa aggattgatt gaaaaatacg atatagatat aatttcaatt
    40741 ggaaatggta ctgcatctag agaatcagaa acttttgtat cagaaatgat aaaagaaata
    40801 gatagcgaag tgcagtatgt gatagttagt gaagcaggag catctgttta ttcagcatct
    40861 gaacttgcaa atgaggaaca tccagatata aatgtatcaa taagaggagc tatatctata
    40921 gctagaagat tacaagaccc attagcagag cttgtaaaaa tagaccctaa aagtattggt
    40981 gttggtcaat atcagcatga tttaaacaaa aagagattag aagaagtact agatggagtt
    41041 gttgaagatt ctgtaaatag tgttggagta gatttaaata ctgcatcata ctccctacta
    41101 gaacatgttg caggaataag taaagctata gcaaaaaata taatagcata tagagaagaa
    41161 aatggagatt ttacgtcaag agcacaattg aaaaaagtaa aaagactagg acctcaagca
    41221 tttactcaat gtgctggatt tatgagaatc ttagagggta aaaatccact agataataca
    41281 ggtgtgcatc ctgaaagtta tgatatatgt aaaaaaatga ttgagataat tggatattct
    41341 ttagatgatg taaagaataa gaatataggt gaaatagatg aaaaaataaa agaaattggc
    41401 ttgagggaat taagtgaaaa actggaagta ggacaagtta cgttaaaaga tatcatagct
    41461 gaaatcaaaa aaccaggaag agacccaaga gaagaaggta taaaacctat acttagaact
    41521 gatgtcttaa aaattgaaga tatacaagag ggtatgactt taaaaggtac aataagaaat
    41581 gttgtagact ttggtgcatt tgtagatatt ggaataaaaa atgacggatt agttcataaa
    41641 tcagaaatga gtaatagttt tgtgaaagac cctatgagta ttgttacagt aggtgatata
    41701 gtagatgtaa aagtcattgg aattgactta aacaaaaaaa gagttgcctt atcaatgaaa
    41761 aaatagataa gtttatgtat ggttatagta cttagattct atatttgtaa ataagattac
    41821 tcgagtgtat aaatgttaaa aatttgtaaa agtatatagt aagaaaatat attaaaataa
    41881 gaacgatagt cttgaaaatc aggaggaaaa tgaatgattt ttataaaaaa tggtaaaata
    41941 aataccataa caaatgggat aattcatgga gatatactta tagatgaagg aaaaataata
    42001 gaaattggag aagatttgat agctccttta gatgtagagg taatagatgc tagtaataaa
    42061 cttgtgtttc ctggatttat agatgctcat actcacttag gtttatggga agatggtata
    42121 gggtttgaag gagcagatgg aaatgaagag actgacccaa taacaccaca attaaatcct
    42181 atagatggaa ttaatccaat ggatagaact tttaaagaag cttttgaagg tggaataact
    42241 tctgtttgta caacccctgg aagtgcaaat gttatgggtg gtcaatgtat agctataaag
    42301 acatgtggaa aaagaattga taaaatggta ataaaaaatc cagttgcatc taaaattgct
    42361 tttggagaaa atccaaagag ctgttatggt caggatgata aatctcctca aactagaatg
    42421 gctatagctg ctttacttag agaaaaccta aaaaaagcag aagaatattt agaggatatt
    42481 gatatgtatg agagtcatga tgatgaagac tgtgaaaaac ctgaatatga tataaaaatg
    42541 gaaagtttaa taccagtttt aagaagagaa attcctttta aggcacatgc tcatagagca
    42601 gatgatatgt ttacagcaat aagaattgca aaagaattta atttaaagct tacattagac
    42661 cattgtacag aaggtcatct tattgtagat gaattagtgg aagaagggtt tcctgtaata
    42721 gtagggccat ctttgtcaga aaggtctaaa tttgagctta gaaatttgac atttaataca
    42781 gcaggaatac tttcaaatgc aggtcttgat gtatgtataa tgacagacca tccagtaata
    42841 ccagttcaat atcttccaat atgtgcaggt attgcagtaa aacatggtat gaaagaagaa
    42901 aaagcaatag aatcaattac gataaatcca gctaaggctt taggaataga agatagagta
    42961 ggctctatag aagttggtaa agatgcagac ctagtaattt gggataattc tccactagag
    43021 attcaaagta atgtacttta cactataata aatggcaaag tagtgtatga gaaaaaataa
    43081 cttgacaatt attaaaaaat taactaatat aagagtgtaa ggaaaattaa aaaatcaaag
    43141 aagatcttcg gggcagggtg aaattcccta ccggcggtat agcccgcgaa ctacttaggt
    43201 ggttgatttg gtgagattcc aaagccgaca gtaaagtctg gatgagagaa gatatttatg
    43261 gaagataaat aagtttattt tagtttgttt aaactatatc cataaaaact cgaagatagg
    43321 gtcttcgagt tttttgtttt tcctaaaaaa tataaacata ttgattaggg ggaaaataat
    43381 tatgcaaaac acagcaaaaa gaggcaatat gatttcaaca agaactttag tagtaatgtc
    43441 aattttatct gcaatatctt atttattaat gtttattcaa gcaccaatac caggtatatt
    43501 tccagatttc ttaaaaattg acttatcaga tattccagca atattcggag gaatgtctct
    43561 aggaccatta gttggatttg gaatagttgt agtaaaaaat gtgttacaag caataacagc
    43621 aacaacaact ggaggaatag gagaatttgc aaatatatta attggtggtt cttatgtaat
    43681 gatactttgt tattcatata agagaagtgg agatttaaaa ggtgtactga ttggttttgt
    43741 attaggaaca atatctatga ctataattgg ttcattagta aattattttg taatgttgcc
    43801 actttatggt caacttatgg gactagatgc tataataggt ttaggttcag ctataaatcc
    43861 tcaagttcat gatttattca cttttgtaat ttggatgata gctccattta atgtgctgaa
    43921 ggctgttata atatctttag taacattgcc actttataaa aaaatgggaa atataataaa
    43981 gaaataatta gacaagaaaa tataaaatta aatgtattga atagccataa tttatttata
    44041 acttcagtcc tatattgtga ttgatatatt aaatatatta tggctttatt aatttatcaa
    44101 aaaatgtata taaaataaga aatatttaga caaatttaaa ttttaaaata aaggaagaat
    44161 taaaacgatt aactattaaa atatgaaaaa ggtatctaca atatacagag cgaatttata
    44221 ataaacaaaa aataaagtta ttattaaaaa actataaaga ttaataatca aacttttaat
    44281 aataactaat ttaataatga taggggtgta tattcatgca tttatctaaa aaaataaaaa
    44341 aattattttt agatgataaa gggtattatt ttaatagtaa ttggaaagat attcatattg
    44401 gtatttatga tacaaaatta ggagattctt taaagtatat cactaatgaa tatattgaaa
    44461 atatggaaag ggtaaaatac gattatcgtg aattaaaaaa tatagaaaat caaactgatg
    44521 gtatgtgtat tttagcaata aggcagaatt acaaggcatt agagtatgta aaaaatcaaa
    44581 ccgaagaatt atgtttagaa gcaataaagt ataattataa agcacttgaa tatgtaaaag
    44641 aacaaacaga atacttgtgt cttgaagcta taaaaaaaga ttgtaatgca cttaagtatg
    44701 tacgaaataa aacagaaggg cttattataa aagcaataag tcattcttcc aatatagatg
    44761 ttgtaagtat actaaaggct ttagagactc aaacaagaag gatatgttta gaagcaatta
    44821 aaaaagatgg caggtgtctt gcatacgtta gagaacagag tgaagaatta tgtatagagg
    44881 ccattaagca aaattataaa gcattaaaat atgtaaaaaa tcaaacggaa aaaatgtgta
    44941 tagaatccgt aagacaaaat ggtatggcat tacaatatgt aaataaacaa actgataaaa
    45001 tatgtataga agctgtcaaa caagatggaa gaagtataca atttgtcaac aataagacag
    45061 aagaaatatg tataaatgct ataagatatt taaataaaaa atataatatt aaagatgtac
    45121 taagttacat agataagtat acagaagata tatgtataga gattgtaaga caaaatggaa
    45181 aaatgcttat gtatataaaa aatcaaacag aaaaaatgtg tattgaagca gtaaaagaga
    45241 actataagtc tcttaagtat gtaaaagagc aatctgagag aatatgtaaa gaagcattga
    45301 agcaaaacca taaagcaaaa gagtatgtaa aaattgctat tgatgattgt atataaggtg
    45361 tagatacagc catcaatagt gaaagataaa aaaagataaa gacaagtact ttataataat
    45421 ctaggcttat actataatta attaagcagt gatataaatt cacatgacga ttatatcaaa
    45481 atggagatga attgatggca aaaatatatt tagaaaatga aaataaaact agagaaatag
    45541 gatataaatt aggtaaactt ttaaaagaag gcagtgttat ctgtttagta ggagatttag
    45601 gagctggtaa aactacaatg acacaaagtt tggcagattc attagggata gaagattata
    45661 taacaagtcc tacttttact ataataaatg aatatgaagg aaaaatacct ttgtatcatt
    45721 ttgatgttta tagaataggt agtagtgatg aaatgtatga tattggttat gatgaatatg
    45781 taaattcaaa tggaatctgt ataatagaat gggctaatct aatagaagat atacttccaa
    45841 aagaatattt gaatatagaa ttgagatata aagatgaagg aagagaaatg atattaactc
    45901 caaaaggaga gttttataaa gagatagttg aggagttgat taaataatga aaatactagg
    45961 tatggatacg tcttctatgg cagcaagtgt agcagttgtt gaagatgata acttaatatg
    46021 tgagtttaca gtaaacaaca aaaaaactca ttcacaaaag cttatgccaa tgatagaaaa
    46081 tatgttgagt atgagtgatt taagtataaa agatatggat ttattagctg tatgtatagg
    46141 tccaggttcg tttacaggac ttagaatagg tatggctaca gtaaaagcta tggcccatgt
    46201 aaataatata ccaataatag cagtaaattc tttggaatct ctagcaaata acataaattt
    46261 ctgtgataga aagatatgtt gtatattaga tgcacagaaa aatcaagttt attcagctaa
    46321 atacaagttt gaaaatggag agatgataga attagatggt gttgatgttg tagagtttga
    46381 tgctttagta gatgaaattg tatcaactaa tgaagaattt ataatagttg gagaagctgt
    46441 atacaaatat aaagacaagt tagagaatat aaaaaatata aaaatacctt caccagctaa
    46501 taatgtatct aacgcaggaa gcttatgctc tttagcatta aataaatata ataaaaatat
    46561 agatgtacat acttgttata cgataaatcc tatgtatatc agaaaatctc aagctgaagt
    46621 acaatatgat gaaaaaatga agaggctaaa taatggaaga taatacaaaa ctaatagtag
    46681 atgatataaa aatagaagaa atgactacag aagatataga tgaagttttt gaagtagaaa
    46741 aaaattgttt tgaagattat tggtcaaaag agtcttttag aaaagagttg tctaatgaag
    46801 tagcaaggta tatagtagct aagttaaatg gaaaaattgt cggatatgtg ggaatttggc
    46861 ttatacttga tgaagggcat ataaataatg tggctgttca tagtgactat agagggaaaa
    46921 aaatagggga caagttgata aaaggcatag ttgatttatg taaagataat aatattgcat
    46981 ctatgacatt agaagttaga gcatcaaata aaatagcaca aaatttatat agaaaatatg
    47041 gatttaaaat gggtggaata agaaaagaat actacagtga taataaagaa gatgcaatta
    47101 taatgtggaa ccaattaaaa gaggtgtaga gtttatgagt gatataataa cattagctat
    47161 agagagtagt tgtgacgaaa cagctgcatc agttttaaaa aatggtagag aagtactttc
    47221 aaacataatc tctactcaaa tcgagacaca taaaaaattt ggtggtgtag ttccagaagt
    47281 ggcctcaaga aaacatgtgg aaaatattga tatagttgtt caagaagctt tagacaaagc
    47341 aaatataggt tttaatgata tagaccatat cgcagttaca tatggacctg ggctagtagg
    47401 tgctttattg gttggtctat cttatgctaa agcacttgca tatactttga atattccact
    47461 tgtaggagta aatcatatag aagggcatct aagtgcaaac tatatagagc ataaggattt
    47521 aaaacctcct tttataacat taatagtatc tggaggtcat actcacttgg ttgaagtaaa
    47581 agattatggc aagtatgaaa ttttaggaaa aacaagagat gatgcatctg gagaagcatt
    47641 tgataagatt tcaagagcta tgaatttagg ctatccagga ggtcctatta ttgataatct
    47701 ggccaagaat ggaaataaac atgctataga atttccaaga gcctatttag aagaagatag
    47761 ctatgatttt agttttagtg gattaaaatc ttctgtatta aattatctaa atggaaaaag
    47821 aatgaaaaat gaagaaatag tagttgaaga tgtagcagca tctttccaag aggctgtagt
    47881 agaggtttta tctactaaag cactaaaagc agtaaaagac aaaggataca atatcataac
    47941 tctttctggt ggggttgctt ctaattcggg gcttagagct aaaataacag agcttgctaa
    48001 agacaatggt attactgtta agtatcctcc tctaatttta tgcacagata atgcagctat
    48061 gatagggtgt gcaggatatt ataattttat aaatgggaaa acgcatgata tgagtttaaa
    48121 tgcagttcca aatttaaaga taaatcagta gagttagatg aatttgtaaa tataaaagta
    48181 ttgtttcaaa aaagtaagta gaagtgaaat taaatataat aaaaataaat atagtatata
    48241 ttaaaacttg aagtaaaact atttataatc tttatattcc aatgaattgc aacaaatttt
    48301 aggatataca gattgtcttt ggcttagatt tgcttcaagt ttattttttt ctatagaaag
    48361 attattttaa aaagtgggta atataccctt ttttttcatt cttaaaaagt tttatactaa
    48421 ttatagaaag attaaggagg ggaagaaatg agtcaaagta aagaagacaa aataagaagt
    48481 attttggaag caaaaaatat taaatcaaat tttcaaaata aagaaaattt aagtgaattt
    48541 aatgaaaaaa aagcaagtaa aagagcagaa gacttattag atgtatatta taatacatta
    48601 tctactgctg atatggaatt tccatattgg tataatagag aatatagaaa atcagatggt
    48661 gatataccag tagttagaag agctaaagct cttaaagctg ctttttcaca tatgacacca
    48721 aatataatac ctggtgagaa aatagtaatg caaaagacac gccattatag aggttctttt
    48781 ccaatgccat gggtaagtga aagcttcttt gttgctcaag gagaacaaat gagagaagag
    48841 gcaaagaaat tagccagtaa cacagctgat gaacttacaa aatttgggtc tggtggagga
    48901 aatgttactg aaagttttgg aaatgtcgtt tcaatagctg gtaaattcgg aatgagaaaa
    48961 gaagaagtac ctgtacttgt aaaaatggca aaggaatggg ttggaaagtc tgttgaagat
    49021 ttaggttttc actatgaaaa aatgatgcca gattatgatt taaaagaaaa cctaatgagt
    49081 acacttatat gcatgtttga ttcagggtat acacttcctc aaggtagaga agttataaat
    49141 tatttttatc ctttaaatta tggattagat ggaattatag aaatggcaaa agaatgtaaa
    49201 aaggcagttg caggaaatgc atctggagat gggcttattg gtatggaccg tctatatttt
    49261 tatgaggctg ttattcaagt tattgaagga ttacaaactt ggattttaaa ttatgcaaaa
    49321 catgcaaaat atttagaaag tatagagaca gatttagaag ctaaaaaaga atactcagat
    49381 ttagtggaga tattagaaca tattgcacat aaacaacctc gtacatttag ggaagctctt
    49441 cagttgactt atacaattca tatagcatca gtaaatgagg atgctatatc tggtatgtct
    49501 ataggacgtt ttggtcaaat tttatatcct tggtatgaac aagatataga aaaaggacta
    49561 attacaaaag aagaggttat cgaattatta gagctttata gaataaaaat cacttgtatt
    49621 gactgttttg cttcagcggg cgtaaatgga ggagtactat caggcaatac atttaatact
    49681 ttatctatag gtggattaaa agaagatggt tctacaggag ctaatgaact agaagagtta
    49741 ttattagaag caagtatgag atgtagaact cctcaaccta gtttaactat gctatatgat
    49801 gaaaagcttc cagaagattt cttaatgaaa gctgctgaat gtacaaagct tggttctggt
    49861 tatccagcat gggtaaacaa ctcaaatgga acaacattta tgatgaaaca atttgctgat
    49921 gaaggtatga ctgttgaaga agcaagagca tttgcattag gtggatgttt ggaaacgtct
    49981 ccagggtgtt ggaaacagtt aactttaaac ggaaaaacat actctattgc aggtggagca
    50041 ggtcaatctg ctggctctgg agttcacttt atagctaatc caaaaatttt agaacttgtt
    50101 cttatgaatg gtaaagacta tcgtatgaat atacaagtat ttgagcctca taataagcca
    50161 ttagatactt atgaagaagt catagaagta tttaaagatt attataaaca agcaataaat
    50221 gttttagaaa gagcaaataa cattgagtta gatatatgga gaaaatttga tacttctata
    50281 ataaattcac tactaaagcc agactgttta gacaaaggtc aacatatagg aaatatggga
    50341 tatcgttata atgcaacatt aaacgtagaa acttgtggta ctgttactat ggtcaattca
    50401 tttgcagcac ttaagaaact tgtatatgat gacaaagcat ttacaataga ggaaatgaag
    50461 gatgccatat taaataattt tggatttaaa gatgcattag aagtaggaaa ttattctatg
    50521 gcagaccaag taaaagtaga taagactggt aaatatgatg ctatttataa ggcctgtctt
    50581 gatgcaccta aatatggaaa taatgatttg tatgcagata atattcttaa aaactatgag
    50641 gtatggttat caaaagtatg tgaagaagca caatctttat atgctaagaa gatgtaccct
    50701 tgccaaatat ctgtttctac acatgggcca cagggagctg ctaccttggc tactccagat
    50761 ggtagactaa gtggaacaac atattcagat ggttcagtct ctgcatatgc aggaacagat
    50821 aaaaatggag tatatgcatt atttgaatct gcaactattt gggaccaagc agtagttcaa
    50881 aattctcaga tgaatttaaa acttcatcca actactataa aaggacaaca aggaactaaa
    50941 aaattattag atttaacaag aagttattta agaaaaggtg ggttccatat tcaatataat
    51001 gttgtagatt cagaaacatt aaaagatgca caaaaaaatc ctgacaatta tcgtcaatta
    51061 atggtacgtg ttgctgggtt tactcaatat tggtgtgaat taggtaaacc aatacaagat
    51121 gaagtaattg ctagaacaga gtatgaaggg gtgtaaaata tgagaaagca tagtgattgt
    51181 atgaattttt gtgcagtaga tgcaaccaaa ggaatttgta gattatcaaa acaaatgatt
    51241 aatttagatg atgcagcatg tccagagata aaagtaatgc caaaatgtaa aaattgtaaa
    51301 aattttgttg aagctaatga tgaaggtata ggcaaatgtg ttggcctaga gaaagaagac
    51361 tgggtatatt caacattgaa tgcaattact tgtgaaggac atgtgtttaa tgagtagtca
    51421 aaagcaatta gaaggcatga tttttgatgt acaaagcttt tcagttcatg atggtccagg
    51481 ttgtagaaca actgtatttt taaatggatg tccactgagt tgtaaatggt gtgcaaatcc
    51541 agaaagttgg actgtaagac cacatatgat gtttagtgaa ttatcttgtc aatatgaaaa
    51601 tggatgtact gtatgtcatg gtaagtgtaa aaatggtgca ttgagcttta atcttgataa
    51661 taagccagtt atagattgga acatctgtaa ggactgtgaa agttttgaat gcgtcaactc
    51721 atgttattat aatgcgttta aattatgtgc aaaaccttac actgtagatg aacttgtgca
    51781 ggtaattaaa cgtgattcta acaattggag aagtaatgga ggagtaacat ttagtggagg
    51841 tgagccacta cttcagcatg aatttttaca tgaagtatta ttaaagtgtc atgaggtaaa
    51901 tatacacact gccattgaaa cgagtgcatg tgtatcaaat gaagttttta ataaaatatt
    51961 taacgatatt gattttgcat ttattgatat aaagcatatg gatagagaaa aacacaaaga
    52021 acaaacaggt gtgtataatg atttaatatt agaaaatata tcaaaccttg caaattctga
    52081 ttggaatgga agattagtac ttagagtacc agttatctct gggtttaatg atagtgttga
    52141 aaatataagt gatattatat cttttatgca caaaaataat ttggtagaaa ttaatctatt
    52201 accatttcac agattaggag aatcaaaatg gattcaatta ggcaaggaat atgagtattc
    52261 tgataagggt gatgttgatg aaggtcatct tgaagaatta caagatatat ttttggataa
    52321 tggtatagct tgttatgttg gtcatgagac agctttctaa aattaaatac aagttttaat
    52381 taaaaaaagg atgttacact aaaagctaga gttacatcct tttttatgtg gtttttatgt
    52441 agaaataatt aagtaaagtt ttatatgttt tatatattgt ttatattgct ttatgtccat
    52501 aaatattgct ttaaatatac tttatatatt ttttatagtt taaattacag ttagagaata
    52561 tattacgctt acttcattga agcagaagtt ttgtggtaaa atactactat gaaaaaattt
    52621 aatagaatat attttagaaa caactaaaaa taatagttag gaggaagaca tctatatgaa
    52681 caaatcatgg agcaacttga ttattaagaa taaagacaaa aaagtacgtt cagggtggat
    52741 tattatagct gttatggcta ccttttacat acttcagtat tatttaagca tgatacttgt
    52801 ggaaataata agaaaaatcc tggttgctac aggtgacatt aatttaaaaa caaactattt
    52861 ttcagaatat gctaattggt taaatgatgt tgggttacag attgtatttc aaattctaac
    52921 agattcactt atgattatta ttcctataat tgtatggaga tttattatga aaaatcctat
    52981 tagtgaaatg ggattacatt cttatagaat taagaaaaaa gaaggatatg taggaatgtt
    53041 attaggaata tttaattgta cagtaatctt tattataaca ataacaattg gtggtggtca
    53101 tgttacttca tgggtaccaa aaatttcacc tttaacagtt tcttggatac tggtctttat
    53161 tttagttgct tttgcagaag aaattttaaa tcgtggtttt tttatggcag ttttacgaag
    53221 atgtaaaaat atttatttta taatgattgt tccatctgtt atttttgggt tgattcatat
    53281 atggaatcct gatgttactt ttttgtctgt aattaatatt ataattattg ggattctttt
    53341 ttcatatatg tttataaaat cgtcgaatat atggatgtgt ataggttatc attttgcatg
    53401 gaatgtattt caggggatta tatatggtat gcctgtaagt ggacttcaag ttccagggat
    53461 tataactaca caaatcactc atggtaatct attaaatgga ggaatgtttg gtattgaagg
    53521 tggaattttg actacttttg ttaatctact tagctttata tttgtatggt attactatag
    53581 agattctaag tatgattttg ttagtgataa agtaaatgtg accaatatca agaattagtt
    53641 gattataata aattttaata atatcttcta agcttaaagt attctgtagg attgttggaa
    53701 atttgcaaaa atataaaaat aacttggata ttctatttac tccaagttat ttttatattc
    53761 ttttgtaaat agaaatggaa cataaaaaat taaaacaacc atagatacta ttagtatatc
    53821 atggtaagtg atagttgatt ctttagaatt tgtgataaga aaagcactat tatttaaaac
    53881 atgaattata gcacaaaacc agatattctt agtttttata taaagatatc ctaaaaatat
    53941 tgaaattcca actatatgta ttgaacgtaa tattattcct aatattggcg cttcaggtgt
    54001 atataatgta aatattagtg gtaagtgcca caagctccaa cacataccta atattataac
    54061 tcctaacttt ttaccaaatt tatcaaaaaa aatattctgt aaaaaacctc tccaggcata
    54121 ttcttcacta aaaaaaatat aaatagtagt taatgatgtt attggagtaa atataaattt
    54181 tatgatacca ctataattag gttgagcccc ttgtagtatt agtataaatg atgttggaac
    54241 aattacagaa atcataaagt aaataattaa taaagaacct agtttaatat tttttacagg
    54301 atataaattt tt
//
LOCUS       DAYATN010000012        52352 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.012, whole genome shotgun sequence.
ACCESSION   DAYATN010000012 DAYATN010000000
VERSION     DAYATN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.012"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(97..2160)
                     /locus_tag="ACGI8A_000702"
     CDS             complement(97..2160)
                     /locus_tag="ACGI8A_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFH0178714.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYSNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(2541..5876)
                     /locus_tag="ACGI8A_000703"
     CDS             complement(2541..5876)
                     /locus_tag="ACGI8A_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFH0178715.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMMDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTENFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(5926..6354)
                     /locus_tag="ACGI8A_000704"
     CDS             complement(5926..6354)
                     /locus_tag="ACGI8A_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178716.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(6344..6724)
                     /locus_tag="ACGI8A_000705"
     CDS             complement(6344..6724)
                     /locus_tag="ACGI8A_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFH0178717.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(6757..7929)
                     /locus_tag="ACGI8A_000706"
     CDS             complement(6757..7929)
                     /locus_tag="ACGI8A_000706"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFH0178718.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVEVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGEPINNTKKFDMKKIKEISIYLKRGVSPKYVEQSN
                     IKVINQKCIYWRNLKVENCKYYDENLKEKIEDIFLKQNDILINSTGTGTLGRCVKFNK
                     IDNSEYIADSHVTVLRSISNNLNSDYFAALFEFTNIQDTIYRKCVNGSTNQIELSVSK
                     LGEYLIMVPPIEFQNQFSEFVKQVDKLKLKIETNLKELEDNFNSLMQKAFKGELFN"
     gene            complement(7933..9396)
                     /locus_tag="ACGI8A_000707"
     CDS             complement(7933..9396)
                     /locus_tag="ACGI8A_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFH0178719.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            complement(9906..10775)
                     /gene="gatY"
                     /locus_tag="ACGI8A_000708"
     CDS             complement(9906..10775)
                     /gene="gatY"
                     /locus_tag="ACGI8A_000708"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFH0178720.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(10795..12711)
                     /locus_tag="ACGI8A_000709"
     CDS             complement(10795..12711)
                     /locus_tag="ACGI8A_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFH0178721.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVAKLNNEEIKSV"
     gene            complement(12714..13634)
                     /locus_tag="ACGI8A_000710"
     CDS             complement(12714..13634)
                     /locus_tag="ACGI8A_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFH0178722.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(13736..14488)
                     /locus_tag="ACGI8A_000711"
     CDS             complement(13736..14488)
                     /locus_tag="ACGI8A_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0178723.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(14880..16325)
                     /locus_tag="ACGI8A_000712"
     CDS             complement(14880..16325)
                     /locus_tag="ACGI8A_000712"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFH0178724.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            complement(16347..16700)
                     /locus_tag="ACGI8A_000713"
     CDS             complement(16347..16700)
                     /locus_tag="ACGI8A_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFH0178725.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(16729..17961)
                     /locus_tag="ACGI8A_000714"
     CDS             complement(16729..17961)
                     /locus_tag="ACGI8A_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0178726.1"
                     /translation="MNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMVFVGSL
                     VTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMETMKNKKFESMSVVAGLAGA
                     GLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGEDSALP
                     DFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFVLLNFL
                     VVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWITLGGL
                     GATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPMWIGTV
                     VISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLLFMIWY
                     PFFKAYEKEEIKLESQEV"
     gene            complement(17984..18301)
                     /locus_tag="ACGI8A_000715"
     CDS             complement(17984..18301)
                     /locus_tag="ACGI8A_000715"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0178727.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            18543..20456
                     /locus_tag="ACGI8A_000716"
     CDS             18543..20456
                     /locus_tag="ACGI8A_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0178728.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGAFILSSKKGYKVNLNTNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(20579..21733)
                     /locus_tag="ACGI8A_000717"
     CDS             complement(20579..21733)
                     /locus_tag="ACGI8A_000717"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFH0178729.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEKLDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(22012..24627)
                     /gene="mngB"
                     /locus_tag="ACGI8A_000718"
     CDS             complement(22012..24627)
                     /gene="mngB"
                     /locus_tag="ACGI8A_000718"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFH0178730.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPDRKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLIAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(24771..26696)
                     /gene="mngA"
                     /locus_tag="ACGI8A_000719"
     CDS             complement(24771..26696)
                     /gene="mngA"
                     /locus_tag="ACGI8A_000719"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFH0178731.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(26826..27581)
                     /locus_tag="ACGI8A_000720"
     CDS             complement(26826..27581)
                     /locus_tag="ACGI8A_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0178732.1"
                     /translation="MNNKIDGGETMEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVK
                     LSIQSLADKFFTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKT
                     LELIDYEKINKVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVL
                     HDIGKLGEQDMFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLY
                     CYSTIKQVNGYNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(27814..28584)
                     /locus_tag="ACGI8A_000721"
     CDS             complement(27814..28584)
                     /locus_tag="ACGI8A_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HFH0178733.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(28586..30034)
                     /locus_tag="ACGI8A_000722"
     CDS             complement(28586..30034)
                     /locus_tag="ACGI8A_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0178734.1"
                     /translation="MKRFLNKMMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVP
                     AISNISNIVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNA
                     GVFGGIIVGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIY
                     PQAAMNQFGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVI
                     RGEIPRFMAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALT
                     SFMATVTEPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSF
                     SYGDKAWLVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLS
                     HGNFSYMAKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLS
                     NTSVQIIIGTDVVKVKDEMEMQLDELRKQADA"
     gene            30404..31126
                     /locus_tag="ACGI8A_000723"
     CDS             30404..31126
                     /locus_tag="ACGI8A_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0178735.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            31323..32990
                     /locus_tag="ACGI8A_000724"
     CDS             31323..32990
                     /locus_tag="ACGI8A_000724"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFH0178736.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(33086..34405)
                     /locus_tag="ACGI8A_000725"
     CDS             complement(33086..34405)
                     /locus_tag="ACGI8A_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0178737.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(34446..35192)
                     /locus_tag="ACGI8A_000726"
     CDS             complement(34446..35192)
                     /locus_tag="ACGI8A_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFH0178738.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(35436..37445)
                     /locus_tag="ACGI8A_000727"
     CDS             complement(35436..37445)
                     /locus_tag="ACGI8A_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0178739.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(37883..39316)
                     /locus_tag="ACGI8A_000728"
     CDS             complement(37883..39316)
                     /locus_tag="ACGI8A_000728"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFH0178740.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(39403..41319)
                     /locus_tag="ACGI8A_000729"
     CDS             complement(39403..41319)
                     /locus_tag="ACGI8A_000729"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFH0178741.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQILITFDIESISKEGYCLETPVVVTNYSDYVDILENTQ
                     KHVNNSQELITVLA"
     gene            complement(41393..42253)
                     /gene="licT"
                     /locus_tag="ACGI8A_000730"
     CDS             complement(41393..42253)
                     /gene="licT"
                     /locus_tag="ACGI8A_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFH0178742.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(42604..43791)
                     /locus_tag="ACGI8A_000731"
     CDS             complement(42604..43791)
                     /locus_tag="ACGI8A_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0178743.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(43805..45196)
                     /locus_tag="ACGI8A_000732"
     CDS             complement(43805..45196)
                     /locus_tag="ACGI8A_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFH0178744.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     regulatory      complement(45340..45519)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(45791..47443)
                     /locus_tag="ACGI8A_000733"
     CDS             complement(45791..47443)
                     /locus_tag="ACGI8A_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0178745.1"
                     /translation="MKDKLQVLSGAMMIPVILLVVAGVFIGLGSAFANMENVSALGLS
                     GLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKKEKGWGALGGIVLFIGM
                     HTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGSFLGIFSYDCSIFGAII
                     AGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLGTAMYFIWPPVGLALSK
                     LGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVGGTMEVGGAIYEGVNNI
                     RLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLADNANKKKAISILIPAIV
                     TAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFNVSIMGESFRNMFPNLL
                     QPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSEEVKLYSKKEYRERQSV
                     DAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNCATRLRVELNSIDGFYE
                     DGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD"
     gene            complement(47463..48866)
                     /locus_tag="ACGI8A_000734"
     CDS             complement(47463..48866)
                     /locus_tag="ACGI8A_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0178746.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(49130..49621)
                     /locus_tag="ACGI8A_000735"
     CDS             complement(49130..49621)
                     /locus_tag="ACGI8A_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFH0178747.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(49647..50459)
                     /locus_tag="ACGI8A_000736"
     CDS             complement(49647..50459)
                     /locus_tag="ACGI8A_000736"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFH0178748.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(50472..51425)
                     /locus_tag="ACGI8A_000737"
                     /pseudo
     CDS             complement(50472..51425)
                     /locus_tag="ACGI8A_000737"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(51549..>52352)
                     /locus_tag="ACGI8A_000738"
     CDS             complement(51549..>52352)
                     /locus_tag="ACGI8A_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFH0178749.1"
                     /translation="KINRFSYKTNFTNGNIMYCGQYIKLPKLGMVKIRDKQVPQGRIL
                     NATISKEPSGRYYVSLCCTDVDIEAFENTNNHIGLDLGIKEFCISSCGEFIENPKYLK
                     KSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDI
                     ICIEDLQVKNMIKNRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICN
                     KCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT        16729 a         9604 c         5535 g        20484 t
ORIGIN      
        1 cttgatataa atttgagcta taaatctata tcaagaggtg ctattttatt tttaatataa
       61 ttaatattct attacgcaaa aaatgcatat atttcattat tttatggaaa atattacttc
      121 atttaactct atatcaaact cattaagtaa aaatctaatt atatttaatt tagcctctgt
      181 atttaaattt gtttctaaat atatattttc tgatatttta ataccttttc ttaatttata
      241 tgcatctcta gacttaatat tatctataaa atctctttta taagttttgt ctacaatact
      301 gttaaacctt acagaatcca aatcatataa agtaagagca acttgttcat aaaattctgt
      361 ccaattttta gccttatatg actctcctaa tatactaaat gatactatac tttcatttgt
      421 aaaattaaca tcatcatcct ctaacgtaaa tatattttca ctgttacttg gaggttcata
      481 tgtaacactt ggatatttcc aaatttctac agctctatct agtaatatct tagttctatt
      541 ttttatttct tcttcattcc atttatctat agatgaaata tatttattta gataaagcct
      601 actatcttta aatccatctt ccatttcttt cttttcttca aatgttttat tacttagttt
      661 tgaattatat cctgtcaaag ttaaatttcc aattgtatgt aaatatttat catgtatctc
      721 cttatagtta tctcctagtg attccttcca tttatttgtt aatgtctgtg gcattatatg
      781 ctctatattt agcatattat tattgattaa attttctaaa tcaacaattt ctttattact
      841 ataattctct aacctctcaa gtatatataa cttattcttt gacttagtat tatatatatc
      901 tttagtttca aaagcatttg aaaattcact gtcagtagga aatctttgag atgatttctt
      961 atttataaaa atatatctta atatattaga atagttttct ttataatcac taaatttttt
     1021 aatttccctt cctaagttca taaatacttt attcagtgca ttcggaggta catcacatac
     1081 tattcttctt aatataaaac attctactaa attaagggat tctacaagta cttttttatt
     1141 tattatacct tcattataat catcaaataa ttctaatata aacggatatg atacaaatat
     1201 ttcaagtttg tttatccttt tcaaaacttt gtttacatca ttatcatcag tacatgaata
     1261 agatatctct ctataatagt tagaaaattt aagtaaatct tttaataaaa tttctatatc
     1321 tataccatta tctgatacat accttttaaa actaaaatat actttatttt tattcggtat
     1381 acttctctcc tttatagtca aatagtctct tataaaacta cttacgttgc tacttgtatt
     1441 tttttcaatt ttataccaat aattattata aaattcctct tgtaacttag agcttctacc
     1501 cattaacaca aagtttctta ctctatcagc ttcacttaaa tctaagccag ttgaatttag
     1561 actttcaaag attaattgag ggtcatcttc accatttttt aactctattt ctactatagt
     1621 taatttctgt atagcatcaa acaaatcatc tattgatata ttatcttctt ttattctatt
     1681 ataaaaatat aaataatttg aaactatatt agaatttcta tctgtattca tttcttcttc
     1741 aaatagttca tagtacgctt ttctatcttc tgctattggt tttaacttta tttttccatc
     1801 tttatctctt gagtgcttgt ttataagata acactcctta atctgttctt ctgttgtaat
     1861 aattcctttt gattccccaa actcttttat tatttgacac atggcaatca ataatattga
     1921 cacagtagtc actctttgtt gtccatctat tattaaatat tcttttccca tactatatat
     1981 agatacaact gaccctaaga aatgagttct aaatccagaa tctattatat cctctaaatc
     2041 atcaaacaat cttttacatt gttctttctt ccagttataa tttctttgat atacaggtac
     2101 tacaaaactt ttgtttgacc cctctaaata actaagaaat tcaatttctc ctgctttcat
     2161 attaactccc cctataagtt aaataaattc cattcataac tccatgtatc tatatattat
     2221 atcaaaattt tgactcataa atgtatatga aagccttatt atataccttt ttatacctgt
     2281 ttttatttta ttaaagtaaa tacatgatta ttctataaaa tatacaaata cttaatttta
     2341 aaaattatta tttaatcttc ttcatgagta attcatagtt atttatcaat taaaaagaga
     2401 tattgcataa ttattcaata cctctctttg tatatttaca ttatccatat ctagttatct
     2461 atttcaaccc aatattagac tacctactaa taaatttatt ctctacatca ttaacaataa
     2521 ttttgcacta aataacatca ttagcctatc tctgatgcat tttctttaat tttatttata
     2581 gttttaatca atttatttct ctcttcaata tgattttcaa ataaatcaat tatacttcca
     2641 acagttctaa atgggtcttc catcaacact ttgttatcct ctataaatcc attcttgact
     2701 acataatcga ctataagttt aacaaaatgt atttgcttag tattaaagct cttattattt
     2761 aaaaactctg aaaaagcttc atttgcagct tctctatcca atcccactag tcttctcact
     2821 aattgactta ctggagtatc tccaaactcc ttcttataat catcacaatt acctaactgt
     2881 tttaagagta tttcctccaa cgtttcaaca tcttgctttg ttatcttttt attatttctt
     2941 aatttaaata ttgctaattc atctttatgg ctatttaaat aatactctac cttcttttta
     3001 taattcttaa gattgtttgc attatataca gaatcatttc tatcttctgc tacaatcata
     3061 tcttcaaaac ttgtataata tatcttttga ctagactttt caagatactt aataagctct
     3121 cttaatgata tacgtacttc ttctacctca aatatatctg aatcttccca aaaattctct
     3181 gtcattgctt tttcaatagt atatttttgt tgctgaatct gtggtatagt cccaagtttt
     3241 gaaagttttt ctgctgtagc cattacatgc cttatacctt tacttgcatt attattttgt
     3301 aagtatgcaa gttgtatatt atacataact acatcaaatc ttttagccaa ttcatcatca
     3361 ctatatgaag ttatgagtgg tgatatatat tctcttatct cattttgtgc tactgctcca
     3421 atattgctcc aaacattttc atttttatac ttatgtacat aacttaaatt catcctaact
     3481 atataactat cctcatttaa agcttttaca gaagttataa gttcttctaa caattctttt
     3541 ctaagttctg catactcacc ttctatatat ttaatatctt gaagttcttt aaccatactt
     3601 acctttatat taaataattt ttcagtaagt gtttctgctt tatttccctc aaatccttta
     3661 ggattaaatt taaaaaattc aaaattatta caaaaatcaa atattaagaa tctctcctta
     3721 tccatatcaa cacctaataa atctggacat aatctagttc ctctacctat catttgccaa
     3781 aacttagtct tacttctcac tttcttgaaa aaaacaagat ttagtatttc aggtatgtca
     3841 attcctgtat caagcatgtc tactgaaact gctatttgag gtaatttatt cttatcagaa
     3901 aaatcatcta ttatagaatc tacataattt atactatagt caactgcttt tacaaagttc
     3961 ccaccatact ttggatacag cttattaaat ctttcaacta ttgccctagc atgctttgta
     4021 ttctttgcaa aaattatagt cttaccaatc ttttcttctc cttctatttt caacccctct
     4081 gtcattagct tatttaaaac taaatctatt gtgtcagaat taaatagcca ctcatttact
     4141 gcattattcc ctatttcgtc tccaatactc tcatcatcat taaatgtatt ttcatattct
     4201 tccttctctt catcagaaag ctcatcatac ttaattccat cttccataat cttagtctta
     4261 aactcaatac tagtataatc tactaaatat ccatcctcga ctgccttatt atactcataa
     4321 gcatatgtag gtactccatt ttccatgtca aaaactgaat aagtattttt atctatctca
     4381 tcctttggtg tagcagtaag acccattaag taagaatcaa aataatcaaa gattgactta
     4441 tactttttat aaatacttct atgcgattca tctattataa ttaaatcaaa atgaccaggt
     4501 gtaaacaatc tctttccatc cttagacttc gtatcatcaa ttgcattcat cattgttgga
     4561 tatgtagaaa atatcatcct agcatcttct gggctgtctt tgctatccaa taaattacat
     4621 aacgccaagt taggtaatag cttagtaaaa ctttttttag cttgctttac caatgctttt
     4681 ctatctgcta gaaataatat atttttaatc caattatgtc ttgaaagtac atcaactata
     4741 gatatagcaa ttcttgtttt tcctgtacca gtagcacata ccaaaagagt tttgcgttgc
     4801 ttattttcaa gggcctcaca tacagcccga atagcttctt tttgataata tctattggat
     4861 atttcatcat ttattgttac agaacttaaa gacttcttac tctttcttct atccatcata
     4921 agttgtaatt ctgatttttt ataaaatcca taaacttttc tatctgaata gtcatcccaa
     4981 atatacatat caaaaccatt agtcaaaaat attactggtc tttgactata tctcttttcc
     5041 aaacaatctg catataattt agcttgttgt tgaccaattt ttgggtcttt gctcgttctt
     5101 ttcgcctcaa ctactgctaa tggcttacca ttttcaccat aaagaacata atctacatat
     5161 cctacaccac tttcattagg cataccttgt acttctattt cctcccctat atcttttcca
     5221 aattcccaac ctgcaagctt taattctata tttatatatc tttttcttgt ctcaaattcg
     5281 cttacttcgt ctatcttgaa atcatagttt acagtattaa cttttctctt ttcagttagt
     5341 gactttctta attcctcatt ttcttttatt acttcttcaa gctttttatc ttttgaactt
     5401 aatctctcgt aaagattttt aagttcgtct ggtctcgtcc ttttctcttc tccatgaagt
     5461 aataaatctt cattaaagtc ttctgctgta tattcatctg aataacaata gtctatccaa
     5521 ctaacaaatt gatgcaaatt gttaagtgaa agtattgctt ctcctctctc tatgttcgca
     5581 ctagtatgta cagataaatt acctaactta actatatatt ttaataaagg taacatatca
     5641 tagtctataa gttctataaa actattatta tgaatcaacg tactgagatt atcttgataa
     5701 ggcaatacta aatcactatc attagcatat agccatttta ctgcaagctc taaagctctt
     5761 cttgttatag ttgcacaagt agatggacta actagtatac ttttttctgc ttctaagcaa
     5821 gcagtattaa aactattgaa atttttcttc ttttgtaaaa aatcaaaatt acccaaggct
     5881 tttatccccc ttttgtccct tagttaaaat aaatctctct aaaaactatt tcttacagct
     5941 gtttcttata ctaacaaata cttcttcatt ttctgtaaac aattcgtaca tatcttcaaa
     6001 ctgtggtata tcatatccat gagaatcatc atttctcaat tttctaagtg tttctataac
     6061 tatagaagaa tttttaggta ataaattgtg atactcacaa gctaaaaggt agtcataaaa
     6121 gtttttagat tttatgttta aatcaattga tttataatac aacattgctt ttgttaattc
     6181 tagaaatgat accatatagc ttttaaagcc ttcttttatg tatctagccc ataactcagg
     6241 catttcttcc ttatcatata agattttgaa gttttgcagg tcatacaagc aagaaccaaa
     6301 atccctattt acttcttcta ttgagttcca agataatttg tttctaatta ccatacatgt
     6361 ccctccaatc taagatatct attacatcac tgttttcaaa atagaattta tttatataat
     6421 ccttaaaatc tttagattca taaataactt caccttctaa aatagctttt ttaagtgcta
     6481 ttgtataatt atctgtaaca atttttaggt ctacatcaat gtctaatata tttcttagag
     6541 cttctgtctt agttgcaagt tctgtaattg gtactttttt gatgggaatc caagctatgt
     6601 ctatatcact ttctgatgta tgtttatctg ttccataact accaaatatt atgattgcta
     6661 gagtattttc ttttggaaat acatttatta ttgagttttt aatctcattt ggtatatttc
     6721 tcatttaatc acctcttgtt tattaggtaa tattgtttag ttaaataatt ctcctttgaa
     6781 agctttttgc attaatgaat taaagttatc ttctaactct tttaagttgg tttctatttt
     6841 taattttaat ttatccactt gcttaacaaa ctcagaaaac tgattttgaa attcaattgg
     6901 aggaaccatt attaaatatt cacctaactt actaactgat aactcaattt gattcgttga
     6961 accattaaca cattttctat atattgtgtc ttgtatattt gtaaattcaa ataacgctgc
     7021 aaaataatct gaatttaagt tatttgaaat acttcttaaa actgtaacat gactatctgc
     7081 tatatattca gaattatcaa ttttgttaaa ttttacacat cttcctaatg tcccagttcc
     7141 agtagaattt attaatatat cattttgttt caaaaatata tcttctatct tctcttttaa
     7201 gttttcatca taatatttac aattttctac cttcaaattt ctccagtata tacatttttg
     7261 atttataact tttatatttg attgctctac atatttagga gatacacctc tttttaaata
     7321 tatagaaatc tctttaatct tcttcatatc aaattttttt gtattattaa ttggctcccc
     7381 aaacatctca ataaatcttg atttaactaa ttcatccaat gcttctattt gctcttttct
     7441 tttatttatt aactcttgag ctttatttaa aacttctacc attttatttt gtatacaaat
     7501 tgtgaaatat ggtattttta tatcatttaa cttgctttta tttaaagttg cacccataac
     7561 tgctctatca gtattattaa aatttaaagt tctcaaagca taatataaat attcagatgt
     7621 tataagttca ttatttttta tttgaaagtt agcaattgct tcatttgtaa acatgtcttc
     7681 ttttaatatg gcaactctac ctatagacaa cttaaaactc attacaacgg tatttttagg
     7741 tactaatttc atattagctt tttcaacagc taaatctgtt atctcttctt ttgattttga
     7801 tatatatttt tctttcaaat cagaaattga taaccatcta ttaccacttc cccaataatc
     7861 agaagtattt ctagatggtg ttttgccaat atttatatag cacaattcat ttaattttat
     7921 atactccata ccctatacct ctaacattct ttctaattct tctaatccaa cagctatttc
     7981 cttctctaac tccctaactc tacctataat cacactagga gcatcataaa ctacttcctc
     8041 atacactacc tctttatact tattaataga taaatcatac ccattctctc ttatctcttc
     8101 cacatcaaca aaaaagcttt gttctgttct ttttctatct acttccccat caagattatc
     8161 aaatctctta ataatatcct ctatatcact atcctttatc tcatttctct tatcatccaa
     8221 agaataacca tcagctttca tatcatagaa ccatacctta tcagttccac ctgtccctgt
     8281 tttagtaaat atcattatag ctgtagatac tcctgcataa ggcttaaaca ctccacttgg
     8341 cattgatatt atagcttcca atttatgatt atcaactatt tcttttctta tcgatttatg
     8401 tccacctgtt gaaccaaaca atactccatc tggcactata catgcgcatc ttccaccaat
     8461 ttttaaaact cttaaaaaaa gtgccaagaa taataactct gttttctttg tcttagtcac
     8521 ttttaataaa tctgctgaca ctgcttcata atctaaactt cccttaaaag gaggatttgc
     8581 taacaccaat gtaaacttat ctttatctgt attaacctcc gataaagaat ccttatactc
     8641 tatattagga ttgtcaacgc catgcaacat catattcata gcacctattc taagcatagt
     8701 tctatccata tcaaatccat agaacatacc attattaaaa tgctccttca gcccttgcac
     8761 taagaaaaga cttgaatgat tatcccttaa gtattgttgt gatgatacta aaaaacctgc
     8821 tgaacctgct gccgggtcta ctataatatc ttctggtgtt ggtttaataa gtcttactat
     8881 catatctatt atgtgtcttg gagtcctaaa ttgaccatta gtacctgctg tagccacttt
     8941 ggataataaa tactcataca aatctccctt tgtatctcta tccttactta attctaaatt
     9001 actaatacca tcaactattt tagataacat aagaggcgta ggaattttaa atattgcatc
     9061 tcccatgtac tttgcataag ctgagtcttt atttccatgt aattttttta tgaaaggaaa
     9121 cacttcattt tgtactattt catacatttt ttctgaattt ccttcattag aaaacttact
     9181 ccatcttaaa tgttgatgtt catcatcaaa tatcctttca aaagaaactc ccaaaagagt
     9241 tgcttctgct tcattttttg tttctacttc atccaatcct ttaataaata aaagatatgt
     9301 aaattgttct ataacttcta atggattagt taatccacca gtccaaaatg tctcccaaat
     9361 cttgtctact ttgctcctta attctcctgt aatcatatat gactttccct ccagtgttca
     9421 taaaataatt taaatatata ttttagtcgt acgccttaat aattagtgtc ttatattaaa
     9481 ttcaaaattt tactacacag ttattaaatt atttttccta tttacatgtt ttagttaaaa
     9541 aactaatgca atactcttat tttatgatta ttatacatcc catacaaaat tattttatca
     9601 tatatccagc atagaagtca tctacctgga taactcttac catattataa ctttttaata
     9661 ttaatttaaa cttagaaaga aattttttat ttaattatat catcttcctt atttcaaatt
     9721 tctcaattac aatctcaatc aaaaaataaa ataagatgct gtctcaaaat agatataatc
     9781 ttctttttta ttttcggaat gatgaaaata tatttaattt catacaaaaa agagtgcctc
     9841 ctgaactaaa aagttcattt tgagacaccc cttaatttaa tcttacttat tctctccctt
     9901 tattattaac acttcactat ctctattgct tttcctacac atccacaaac ttccatccta
     9961 ctcttaacaa aatctttaac tgactctaaa ccttttttac catatttctt agggtcaatt
    10021 acctctgggt cagaattaag cacatcctta accccattgc tataagcaat ccttaattca
    10081 gttgcataat taaccttgca aatccctctt tttatagatt ccttaataac ttcatctggt
    10141 attccagacc caccatgtaa tactaatgga actgatacaa catctctaat ttcagataat
    10201 ctatctaaat caagctttgg ctctccctta tataatccat gtgccgtacc tattgctact
    10261 gccaaagatg atacccctgt tctctctaca aactcttttg cttctaatgg gtctgtatat
    10321 gctcccccat caccaccatc taaatcgtct tccttacctc caactttacc taattcagct
    10381 tcaacaggta tatctgaagg catacatgca tctacaacag atttagatat tgaaatatta
    10441 tcttcgtaac tttcatgaga accatcaatc ataatagaag tatatccaac tctaagtgcc
    10501 tgcatagcta gtccaaagct tgaaccatgg tctaagtgca ttgctactgg aacacttgcc
    10561 ttctctgatg ctacctttac attcgctaaa taataatcta gccctgcata ctttattgtt
    10621 gaaggtgttg tttgcaatat tactggtgat ttaagttctt cagcagcctc tattactgcc
    10681 ataaccatct ccatattttc aacattaaat gcacctactg cataatgtcc ttcttgtgca
    10741 tctaataata tttgtttagt tgtcactaat gccataattt ctcctcctat atgtttatac
    10801 tgatttgatt tcttcgttat ttaattttgc tactatttct ctacttgttt tgctatttaa
    10861 taactcattt aaaaattctt cattcattaa ttttgaagat agttgagata atgtttttaa
    10921 gtgtaaatta tcaccattat ctgtagctgc tatcataaat ataatatgtg ctaagcttcc
    10981 atcttctgat tcaaagtcaa acccttcttt tgatacacct actgctacac gtggtacctt
    11041 tactgcatct gtttttgcat gtggtatcgc tatccccata ccaaaacctg tagatgatat
    11101 ttcttctctt ttaaggattt cttctttaaa cttcttttta tctaaaagaa cactagattt
    11161 atcaagtatt ccaatcattt catcaattac atctgtttta ttttttccct taacatctaa
    11221 aattactgtt tcttcgctat ataaagagat attattagat acgttttccg attctgcact
    11281 ttcattttta tgttcatctt ttgttacatt tgtattttct atagtatttt tctcatttac
    11341 tactttaggt gtaaccatca tataaagtac agcacctact aatgaaccta caaatataca
    11401 ccctacccat aaaagtggct tattgactaa tcctagaacc aaaaaccctc catgtggagc
    11461 tggtacttct attttcatta tataagttaa aattgatgat attgatgatg caatcataag
    11521 tactggcaac acttttaatg gattttttgc tgcaaaaggt attgccccct ctgttatatg
    11581 agtactacct agtatataat tgattaatcc tgctgctcta tcttcttctg taaatctatt
    11641 tttaaataaa gtagatgcta ttgctattac caatggtggt gttatacaag ctgcagatac
    11701 acctgccata aataaataat ttccctgtcc tagaagcata gttccagtta catatgctgc
    11761 tttatttact ggacctccca tatcaaatgc tgacatacat cctattacta ttcccaaaat
    11821 tatagggctt gaattttgta accccgatag aaagttcatc atagcattgt taagtgctga
    11881 acatggccca ccaagtaaca acattgctac acccacaaga cctacgctga caataggaat
    11941 tataaaaatc gatttaagac cttctaattg tcgtggtaat tttcttaaca aatgtacaaa
    12001 tagtttaact aagtaacctg ctaaaaatcc tgatatgatt ccacctaaaa accccgaacc
    12061 tgtatcaaat gatatcattc ctccaacaag tcctgctaac ataccagctc ttcccgatat
    12121 agattgagct ataaatgctg ccaatacagg aaccatcaag ttcatagaat atccaccaac
    12181 agattttaat gtagctgcta tttgattata ttgttctgaa tttgggtcaa atgaataaat
    12241 cccccataaa aatgatatag cagtaagtac tccacctgct actactaaag gtaacatgtg
    12301 agatacacca ttcattaagt ctttatacaa cttttgacca aacgataaat ttccttcttc
    12361 tgcaatttta ctgcttgata agtttccttt tctaatagct acttttccat caatacatct
    12421 ttgtataagt tcattagctt tatgtatacc ttctttgaca ggtacttcta ttactggctt
    12481 tccattgaat ctgtccatat caacattttt atcacaagca actattattg catctgccat
    12541 ttcgatatct tttgctgtta acttattttc tacacctata gctccattcg tttcaacttt
    12601 tatgtcacac cctaattccc tggctgaatt ttttaatgct tcttctgcca taaaagtatg
    12661 agctattcca gttggacaac ctgttacccc tagaatcatt ttctttccca tttttatacc
    12721 acctttctga ctcttatatt ttttatataa tcttccacat tctccaaatt acctatacca
    12781 ttactttcag ccacacttgc tcctgttgct gctgaccttt ttaatgcttc ttcaatatgt
    12841 tctggatttt ccaaccatat acttaaaaaa gcagctaatg cagaatcacc agcacatgca
    12901 gagctgacta ctgtaactgg ttgagcacta gcataataaa ttgattttcc attataaaaa
    12961 taagaacctt tttctcctaa agttagcaat atattttgag caccttttaa atgtagtaat
    13021 ttaagtacat ccttaatatc ttcttcatct cttacaataa ttccaaatat atctttgatt
    13081 tcttcatcat ttggttttat caaatatgga ttatactgta gtaactcctt taattttggt
    13141 gaacttatat cgagtattgt ctttatactt ttatttttac aaattttaaa tatatcctca
    13201 tagtaattat catcaatacc aggaggtaga cttccactta ttgataagta acttatatct
    13261 gctaatgact gtatcttatc tagcatatct agtttttgtt tttcatttac atatgaccca
    13321 gagtttacaa atttaaattc atcacttcca tcatttaaaa atatatttat acgagttgta
    13381 tcttctaccc aagtaggcaa tacgtcaaat cctctctttt cgctttcttc aactatatat
    13441 tttcctgaaa atcctccaaa aaatcctaat atcttagatt ctataccata atgacctagt
    13501 acaaaagtta catttaatcc ctttccatta ggagaatata cagcatttga tgtacgattt
    13561 actaattttc tctttatccc attagtagat atattcatat ctattgcagg atttgtagtt
    13621 aatgtatata tcattgtctt cccccttgct tgtgctgtgt atgttttcat tataataaaa
    13681 aagtctatga aaatcttttc acaaatagaa aatcgatttt catagacttt tcaatttaat
    13741 tttcagaatt attatatctg accatataat ctataattat cctagatata atatacaagg
    13801 gcaatctgga agatacttca tattcagaaa ttgatatatg ttcatgtttg tatcctacaa
    13861 tatataaatc cgaatacttt ataagttttg cactttcact acaacagcaa gttataacct
    13921 tagctccaac taaattggca ttctgtgctg attctacaag ctctatggta tttccattca
    13981 atgaaaatat aattataagt tcgtcttttt ttattgattt agattttatt ttcattatgt
    14041 tggggtcatt tataaaaaca acattaaaat caagtaattg caatttaaga ctaaactctt
    14101 ttgctacata ttcagtaagc cctcttgcta aaatatatat cttactagat ttatttatag
    14161 tttttattat attgattagt gtagttaaag atatttgttc tataactctc tgtgcttcta
    14221 taaaggattt atttattata tcccccatat tttgtatatc atcattttca tcttttactg
    14281 ttaatctata cctaagttca ttgaatccat ttaaaccaca ttttctaatt gctcttgaaa
    14341 cggttgctgg agatgaatat gtttcatgag caatctctac tatagataat tctggtaata
    14401 ttgattcatt ttcatttata aatttaatta tttcaagttc cgtcttcgtt agattttcag
    14461 aattactttt gtttagctca attatcatat gttatctccc cttatataag ttttatttgt
    14521 ctaaatttat cgagtaatat aatatattac ctcttaacta cttattttta tatacaatta
    14581 agatttagtc aatgtctata tatgtaaatt tataatataa taattttttg cttaaagttt
    14641 attatgttat cttatagcat tttaaaataa aaaagaattg tacctctacc aaaatttgga
    14701 tgaagtacaa tagatattcc tcatactgat atgtttatga ttaaaagtat aaatataaat
    14761 ttcgctatga atcatataat taataaatca ttttatttac aattataacc ccccttataa
    14821 aactatatta ataatatata cagtatttat aaggggggtt gtttatattt aaaaactatt
    14881 tacttttctc ttaattcttc tccattagtt cttattacat tttgatacca ataaaaacta
    14941 tcttttttat aacgttcttt acttccattt ccattgttat ctatatccac atatataaat
    15001 ccatatcttt tttccatttc ttgagatgaa caacttacta tatctattgg cgaccacata
    15061 caataagcta atatttcggt tccatcagct atagcttcac ctatttgttc tatatgtttt
    15121 cctaaataat ctattctgta actatcatga acttttccat cttcaaattt atcatgcatt
    15181 ccaaatccat tttcagctat cattattggc ttttggtatc tatcataata ttgagataaa
    15241 gtattgtata atccttttgg gtcaattgcc cacccccaag gatttgcttt taaatatgga
    15301 ttctttatat tgtcagttgg attcatggtg tttttacttg ctgatactgt tgtagaataa
    15361 tagtatgata aagctagaaa atccatagta ttttctttta agattttatc atcaccttcc
    15421 tccataacta tatttatgtt attttctttg aaaaatctct taatatagac tggatactct
    15481 cccctaaaac ttacatcagt gaaaaaatat tgtagcctat ttctttttaa agcaaatact
    15541 acattttcag ggtcacaatc ttctggataa gatgtacaat ctgccatcat catacctatc
    15601 atcatatctg aatttatttc ttttccaagc ttcttagtca aagctgtagc tatgaattga
    15661 tgatgaatag cttgatatct agtctcttct acattatcaa cttggtctac acaaaatgca
    15721 actgaattaa atgattcata atgtattaaa tttatttgat ttataactat ccaatattta
    15781 actttatttt tatatctatt tagtattaat tctgcatatc taacaaagaa atctaccact
    15841 tttctattag accatccttt atattttaat actaaattca aaggagtctc atagtgaagt
    15901 agtgttacaa taggttccat accattatct attacttcat ttataagatt atcataaaac
    15961 tttaatccct cttcattagg ctctaaatca tctccatttg gaaatattct agcccaattt
    16021 attgaaaatc taaaacactt taaccctagc tctttcaaaa gctttaaatc ttctttgtat
    16081 gtatgataaa agtctattcc actacgctta ggataatatc cattattatc tttcatagca
    16141 aactttattc tttctatagt catatcagac tcataaggtt tagatatatc tctatcttta
    16201 tcatacatat gtacatcagc taaacaccaa cctttgccac cttcattcca tgcaccttct
    16261 aattgattag ctgctacagc tcctccccat aaaaaacctt taggaaactt acccaattta
    16321 atcactcctt attttattta tttaaatcat ttactattat ttaccttata ttctatagac
    16381 tttactattc ctactaaaga catagttata tttaactcgc tgaatatggt cataagagta
    16441 tcttgagcat gtgcaaataa cagcgaatgt gctcttttct ctccaccagc ttccccttgt
    16501 ataatcttgg tttgagctct atgtgcttct gtcataaacc cttttgcttt agctagtttt
    16561 aaatcagcat ttctataatc aaacttttcc aaatcattaa gagcatgttt aatttctgtt
    16621 ctagcctcac ctgcacgaag tattatttgc attgctactt ctactaattc ttcatctatt
    16681 atatgttctt caattgtcat atttataatt tcctttcaat ttctaaattt aaacctcttg
    16741 actctctaat tttatctctt ctttctcata agctttaaaa aatggatacc atatcataaa
    16801 tagcaatact atatttattg ctaaaacaat aagacctcta acatcttgat ttaccataaa
    16861 agtagatata ccaaaaggaa cataccataa attaaacacc ttacttggaa ttgtaacaaa
    16921 tcccatactc attgataaat aagtagttat tgatattact actgttccta tccacatagg
    16981 taacattaaa aatgggttcc atgccactgg agctccaaat attactggtt catttatatt
    17041 cattatagaa gggacaatac atgctcttcc tattgctttt attcttccaa ctctggataa
    17101 acacatcatt ataactaatg gtaatgtagc acccaaccct cctaaagtta tccagcctgt
    17161 ttgaataact tcgtaaacat atatattagt tgctactcct cctgatgcta ctgctgctgc
    17221 attagctgca attgcaccaa gttgaattga ataagttata ggagatagta cccacccact
    17281 tattcctaaa gaatatataa atactactaa gaagtttaag agtacaaatc caggtaatgt
    17341 ctgtgctata tctgatactg gtttaaatac tctaactatt atttcaaaca tgtcaaagtt
    17401 gagtccataa attaatcccc agcctacaaa cattatcact gttataggta acatattatc
    17461 aaaccacttg cttacaaaat ccggaagtgc tgaatcttct ccgaaaaatg aaaaagaacc
    17521 aaataaagac attattaatc cagagaataa tcctgcaact agcgatacaa gcattccttc
    17581 tcctccaaac ctgttgaagt taaatatagc tccttcattc gtaatctctg gacgaatcat
    17641 cattaacatt aatccagcac ctgcaagacc agctactaca ctcatacttt caaatttctt
    17701 attcttcatt gtttccatag gcaataaaaa tgctataaat aatcctaaaa gtccaaatgt
    17761 atattgattt attggactta aatcaggtaa aaagcttatg taattcttca agacagaaac
    17821 tatagttact aatgagccta caaataccat tggcaaaact ttcattatag ttgattgaac
    17881 tgattgtacc catatattct tagtcatatt atttacaaca ggagtaaatt tttcacttaa
    17941 aaaatctata aatttattca taaacactct ccccataaat attttatttg tttgatatta
    18001 gttctagtgc ttcttccaaa gcctttgcac catctagtct tccatatgca gttttagata
    18061 tacatgctac tggaacgtta tattcattag ccttttgttg aatttcttcc ttcatataat
    18121 ctaaatgtgg tcctagtaat aaacaatcta tttctgataa atacttatct atctctgact
    18181 cacttctcgc ttttatatct gcttctatac cttttttctt tgcactcttt ctcatactag
    18241 ttgccataaa acctgtactt gctcctgaac cacacgccaa aagcactctt atatttttca
    18301 taataatacc tccaatattt ttatccatta atttatttct taatgaactt tttattttat
    18361 tatgtttata ttttacttcc caaataagtt catttcacat aaatatattt cctaataata
    18421 ggaaatcatt tactcttaat tagacctatt aatatagtat aatgtatctt ataatacgta
    18481 cattcaatta tataattatc atatcataca gattttattt taaaactatg cgagggaatc
    18541 taatgaaaac atctaaaaaa gagctatttt taaatttcat aaaaacgcaa catgattgga
    18601 tagattcatc cacattagct aattacctta atgtatcaac acgttcgatt agaaaatatg
    18661 taaacgagat taattctaat ggtgcattta tattatcttc taaaaagggc tataaagtaa
    18721 atctcaatac taattgtcaa actaagattg atagttcaga gaatatatca cctgataaca
    18781 gattaaattt aattttaaaa gaacttatag ttaatagtaa tggaattaat atatttgatt
    18841 taagtgaaga attatttgtt agcccagcta ctattgaagg ggatatagtc aaagctaata
    18901 aatttatagg aagttataat ttaaagataa agcaatcgaa atttttattg aaactagttg
    18961 gaaatgagtc tgcaaagcgc aagttaatga gctctataat atttaaagaa acgggaagtg
    19021 attttttaag cttatttgat gtacaaaaaa tatatcaaga gtataatttg actaaactaa
    19081 aagaaaatat aatatatata ttaaaaaaat acaatttatt tataaatgag tatgctataa
    19141 acaacatact tcttcattta atgataacta tagacagaat aaaaaagaat aattacatag
    19201 actctgttga agtagtaaac tacatagaca ataacgtaga tataaatatt gcaaaagaca
    19261 tatcaaattt tttagaatct gaatacaata taactttaac tagtgcggaa ttatactatt
    19321 tagtatttca gttaacaaac aaaactacag tcttaaacta caatcaaatg gatactaaat
    19381 cattgtcaaa ttatatagat gaacactttg ttaaattaac taaaaaaata attaaaaatg
    19441 tatatgactt atatcttata gatttatctg atgaggaatt tgttgtaaaa tttactcttc
    19501 atgtaaaaaa tttaatcagt agagccaaaa ataatcaagt tttaagaaat caaatacctc
    19561 aaaagttaaa agattcctac cctttgatat atgatatatc tgtttatatt tgcaatcaga
    19621 ttcaaacttt agaaaatgta gatatagacg aagatgaaat atcttatata tctcttcatg
    19681 taggttcatt ctttgataga caaaaattat tagaagataa agttctatgt gcattaatta
    19741 ctccaaatta ctatgattta caattcaaaa tagtaagaga ccttgagaag agatttaatg
    19801 aatccataga aattattcag attttttcag atactcataa tcttgatttt gacaataagg
    19861 tagacatggt tattactact ttaccaatta acgatagatg ccctatacct tttgtttatg
    19921 taaatcctta cctaaataga aaggattatg ataatattca aagtaaattt attcagatta
    19981 aagatagaaa aaatatactt actgtacaaa atcatttaga aatgtacttt agtgaatctt
    20041 tgtttatgaa aaatatatat ttagatagtg ctaaagatta tataagattt atgggaaata
    20101 ttctgtacga aaataaatat gtcaaaccca attatataga tgatgttctt ataagagaaa
    20161 aaatgtcttc aactgcattt aataacaatg tagcaattcc tcatagcatg aaaatggatg
    20221 ctttaaaaac cggagtatgt ttaattgtta acgataaacc agttaaatgg ggtgaggaaa
    20281 aagttcaaat tatagctatg atacctataa atgaaaaaga aaaagaaaaa ttcaattaca
    20341 tatttgaaag ctttattgaa atattatctg aatggaataa tgttaaagaa cttactaagg
    20401 cagataacta tagtagtttt atgaatagaa tagcttatct tattcaaaat atctaaatcc
    20461 attaatttat ttccaatata tgtttcttaa gttattaaca taaaatagat aagctgtcac
    20521 actaaaaagt ctaaattaat atgtgacagc tttttaccta atataccttg ttttaatact
    20581 acatattttt tgactgttgt atactagtta taagattgaa tatttgtgtt gttgtagctg
    20641 tcatattctc actagcagtt gctttatcca ttgcttcttc tattgtcata gctctattta
    20701 ctatagaaaa ataagcatct ataccctctt cattacattt aactgcatct tctgttgtgg
    20761 ctccagccaa acctattact tttaccccat atttttttgc cagttttgca actcctattg
    20821 gagccttgcc catagcagtt tgattgtcca atctaccttc accagttatg acaaaatctg
    20881 catcttttaa ctcttcctct aatttaattt cttctaatat tatttttatt ccagattcta
    20941 attttgaatc taaaaatgct aaaaaagcaa aacctaatcc tcctgctgct cctgctcctt
    21001 ctacattagc tacatcttta cttaagtaat ttttaactac tttggaaaaa tttctaagcc
    21061 cattatctaa cttctcaatt atttctgatg ttgcaccttt ttgagacccg tatatgtatg
    21121 cagctccatt tttcccaaac agcggattat ttacatcaca ggctatttta aacttacatt
    21181 catctaattc ctttaatcta ttttctattt ttatatgtct tatttcattt aaaacttttc
    21241 cacctaatcc tactaacttg ttatttttat cataaaactc aaaccctaag gcttgaagca
    21301 tacctatccc agcatcatta gtagcacttc cacctatccc aactataaaa tttctatagc
    21361 ctttatttat tgcttccttt attatttccc caacaccata agttgtagtg tataaaggat
    21421 ttctaagttc agtcggaacc aatgttaatc ctgatgcttg agctatttct attattgctg
    21481 tacttgtatt ttttaataat ccatatgttg catccacttt tttagctatt ggtccagtaa
    21541 cttctatagt ctctttttta ccattcatcc cttgaactaa agcatctact gtcccttcac
    21601 caccatcagc aagtggcaat atctttactt gcgcatcttc tttaaccttc aatatcccct
    21661 tttttattgc atttcctgcc tctattgaag ataagcttcc tttcaaagaa tctatagaaa
    21721 ttaatatttt catacttatc tactcctttt tattactcct tattttaaac atataatttt
    21781 ataagcttaa tttatctttt attttatact ctacatctct ctttgtatac aacttatacc
    21841 attaatattc ttttatctta taaaaaggga gtgtctcaaa ataaatttct tagttcatga
    21901 gacactcttt tgtataaaat caaatatatg tttattattt caacaatgat aaggaggatt
    21961 atctctattt taagataatt cctttttagc actatctaat catttttagc actatttaac
    22021 cagtatagtt ttaacttggt tcattaagca ctctacattt acagagttat tatctattga
    22081 tatagattct aattcttttt catttaggtt aatccattta gctgttttaa tatttttgtt
    22141 aaatactatg ttagctgatt ttgtttcttc tttagaatta taaaatctaa ctactaatgc
    22201 ctcttctttt tcagcctttt ttacaacact taacacaagg tccttattag tttccttaag
    22261 taaactatat gaataatcta cttctatttc tgattgattc aatttcatag cattatatgg
    22321 cattttatta taagtttgta tttttgttaa atactcttta gccatatttg caacatttgc
    22381 ttctagtgta gatttttcat gagtaactat tgctaagtct atttttatat ctcctatcat
    22441 ttgagaatct ggagtaggca actttattcc tgatggacga cctgggcgtc ttgccatttc
    22501 ttctttacct aggaatccta cacttctaaa taatgttata gctattgtgt caaatttttc
    22561 ccctactatt tcaaactctc ttgtactgtt tgtaagaaca gctactccat gctctttgtc
    22621 agacaatcca acgaatgtta gcattggata tatagaatct ggtctttcac tccagtcttc
    22681 ctgttgccaa acttccatag catcatcata cacacttctc tttatatgtc caaactgatt
    22741 atcagacaca gaaaactttg aagctatatt tgttggtata taagttctaa ccctacaatc
    22801 tcttgcttgg ttatttatgt tgaatactac atctattata gctttatcat ttggaacatt
    22861 tataactata tcaaagtcaa taaatgaatc tgctctatta ttttttctag cttgtatatc
    22921 ttttggtact tttaatctat aatttatctt tatctcatta ttaaatctat ttttctttat
    22981 agtggaattt gctactatat tatcgttaaa tattaatttc tcatttggaa gtggtgaaaa
    23041 atcatactca tctccttcat ctcctccatt ttccatcaat aatacatttt caaaagtttt
    23101 atttaatttt ttatcaaata tctttaatgt cccgttttca ttaacttgaa tagtataaaa
    23161 gtcattatct accatgtcac atacttcata agctttttct aacatatgtt caacttcttt
    23221 tatcatatac gccttatacc ccattgcagg tattttatct ttaaagttta ttgtatatac
    23281 cataaatgaa tcatagtttc cataatgaac aatttgacgg tctattaatc ctgcatctat
    23341 tacttcttta tgtaccactt caaaatccaa cttattatta tctttatcaa atatttcaaa
    23401 tgctttcatt ttagttataa cttgggcgct cactatttca ttcctatcgt aaggcattaa
    23461 gttaaatgct attaacttat ctaaagccat ttcacaactc attgcatcaa ctattcttct
    23521 tttgtaaaat gttattaatt ggtctacttt ttcctcagct aagaaaaatc tattcattat
    23581 ttcctggtga actttatctg aacaacaaca tccaatgctg tcatgagcat gatttttcat
    23641 tatttctttc caaattaact ctattaatcc atgatgatat tcaaatccta gagaatatgc
    23701 aatagaagct agtggctcca atatgttagt tatctttgat tctattctag catttgctga
    23761 ttttatatcc attcttgttg agaatatact tctatgaacc ctcatatatt tcccatctaa
    23821 aaattcccct tttattgtat ctatatcctc atttttttct agctctttaa atatgttttc
    23881 atatctactt aagaaaaatt ttctgtctgg ataacattct tttaatttat atataacttc
    23941 aaatatattc ttttgaattg gcatttggtc atgtccattt ggaagtatta tatggtcagt
    24001 agttgcaccc ttatcaagaa ccggtaaata tttatccatt cttgttctta gctcatcttc
    24061 attcattggt agatattttc ctattgcata acctaatgga agtatttgaa ccaaaacaga
    24121 agacccatca tcactcttcc aattaaactc tgttttatta gttccttttc tttcagaaca
    24181 tcctctccaa aacattgaat attttatatc aaacccattt agtatttgtg gcatttgagc
    24241 actttgacca aatgaatctg gtaaatatcc tattttcata tattctccaa attcatcaca
    24301 atcttttatt ccatatagta aatttctaag tatggattct ccaccaacta ccatttcatc
    24361 agtttgagta taccaaggac ctattattag ccttccttct tgaactaatt tttttattct
    24421 ctccttacat tcaggcttaa ctgctaaata atcttctaat atagcagttt gaccatccat
    24481 cacataatat ggatagttat gattattttc taacatatcc attatttctt ccatattatt
    24541 tacaagtaat attctagatt cctcagttga gaagtaccat tctctatccc aatgcatatg
    24601 aggaactatg tgtacatttt ttttcattgc ttatcctcca aaacttatct gataatattt
    24661 tctgtctaat taaaaatcca ataaaaatac tactgagttt acaaaatttt ttaatattta
    24721 ttttatcact ataattaaac ctttgattta aatatctaat aacgtatctt ttaatgtatc
    24781 tttctttgat ttttttgttt cctagttatt attaataata ttgctgatat tatagctcca
    24841 agtactgccg caaagaacca tattgatgct ataataaatc ctgacatact tccctctaaa
    24901 agcgccaatg agaatatccc cgctccaggc acttgaagac ctatattgaa gaatgctact
    24961 atagacccag ttatggctga accagctacc aatgatggta taacacggat tgggtcctct
    25021 atcataaatg gtattgcacc ttcagttatt ccagccaatc ctaatatcca agtttgcttt
    25081 cctatttcct tttctgattc tgaaaataaa tctctgccta aaacagtagc tattgtaact
    25141 ccaaaagctg aaaccatctt aactgatgca aatattgcat aaggtataaa atttccactt
    25201 gctattgctc ctattgcaaa tgtataggct gctttattta ttggtccacc taagtcaaat
    25261 gaaaccatag ctcctattat tgcacctagt agtattgcat ttactccagt catcccattt
    25321 aaccaattta taagaccatt gttaagtgct gctacaggct ttcctactac aaataacatc
    25381 aataaaccta ctaccaaaga accaactact ggatataccc aaaaacttac gaatccagct
    25441 aatgtgcctt tggctggtat attcttcttt aaaaatttta ctatataacc agcaagtaaa
    25501 cctcctaaca tacctccaag aaatccacta cctattaaat cagctgctag acctgctgca
    25561 aatcctggtc caagacctgg tttttcagct agtgagtaag acatatatgc tgataatact
    25621 ggaaccatca atgtacctaa catacctcca gaaagctttc ttaattgcca taaccaagaa
    25681 ccttcttgat tgtatagttc ttgtagacca aatgcttgag atgctaatac tgcaaatgca
    25741 agtatcatac ctcctgccac aataagtggt attatatgag atactcctgt aagtattgct
    25801 gattttacat cttttttcca tgatgatttt tcacaatatg atatatcaga tgatttttct
    25861 atataatcac ctttaccttg tttttgtgct acttctaaag cttcgtatat tatttctttt
    25921 gctcttttta gtggagctgc tactgacgtt tttatgtttg gcaaatgttc aaatctttct
    25981 gtatttttag gtgctacatc tgttgcatat attattgcat ctgccctttt tattatatct
    26041 ggcgtatgag ggtccaccat accattagct ccttgctttt ctacatacac atccacacct
    26101 atttctttac cagctttaat taatgcttct gctgacatat atgtatgtgc tataccggca
    26161 gggcatgctg ttattgctaa tataattttt tttgattcat tttgaatcct aatctcttct
    26221 tttatttgtt ctttcttttc ttttatatct aagttattaa ataactcttt cttagttttt
    26281 gaatttaaca acatattcat ataatcttca tttgacagtc tagtaactaa ctcagaaagt
    26341 atttgtatgt gtgtatttcc accttcactt tctggtactg ctattaaaaa tattagctct
    26401 actttattat ttgggtctac gctttcccaa ttttctattg gtgtctctaa ggtcatagct
    26461 gcaaatgcag cttctttaac tgacttagat tttccatgag gaattgcaag ccctctttca
    26521 aaacctgttg gagataaagt ttctctgtac attacagctt ctaaatattc ctcataagat
    26581 gttaacttgc cttcatcata tagctttctt gctaagaact ttattgcgtc ttcctttgtt
    26641 tttatttttt gttttaaaaa aacaaggttt tcatttgtga ttttcttaaa ttccaaaatt
    26701 acgccctcct taaatcattt aatgctatga atatagcata aaataaatta tgaaaatgtt
    26761 tttttgaaat tatacacaca atttcttctt aaaaaataaa gatttgtgta tttatacaca
    26821 aatctctatc ttaaccaata ttgctctatt attgctctca taatcatcat aattggaacc
    26881 ttatctgata tattgtaccc atttacttgt ttaattgttg aatagcaata taaacttata
    26941 tctgcaattt gttgcaattg attttcacat atatgtgtta aagctactac cttacaaccc
    27001 ttattaactg ctgtctttgc tgcttctata acttctttcg tttctccact taaactaagt
    27061 gcaaaaaaca tatcttgttc acctaatttt cctatatcat gaagtacttc atgtctttga
    27121 ctatagtaag ttatattatc atgcccacct ctaatttctt ttactaatat ctcacataaa
    27181 ggaatgtttt gacctattcc ataaactata atactcctag atttttgaat tatatctgct
    27241 actttattta ttttttcata atctattagc tctaatgtct tttttatatc tgattcaaaa
    27301 ccattttttg gctctttatc ctcatttttt aactcttctt ttaaattatt ttttagttga
    27361 gagaaaccat catactccat ttttttacat agcctaacta tggtatttgg gacagtaaaa
    27421 aatttatctg ctaaagattg tattgataat tttactattt cttctttgtt ttttctcata
    27481 tattcaatta tcatgtcttc tgtatctgta aatttatact catatttttt tgctctttca
    27541 taaaattcca tagtttctcc tccatctatt ttattattca taagtaaaat tcaaaaatat
    27601 tagctaaaat tttcgttaat ataattataa tttaatattt ttaatttatt aatgcatact
    27661 actcttttta ttatacataa aaaatgtgta cttcctatat tgatagagat gattcaagtt
    27721 tttattttga tacatctcta tcaatatttt tttagaaaat acacattttt ttcaaatact
    27781 tatttttaat taaaacttta ggtaatctat tctttatata taattattag acttgaaaat
    27841 atctttaaaa tcaaccaatt caatattatt tctttttaac cacattttaa cttccttatc
    27901 acatagcatt gcatgttcat atactctctc ttgaataaga gaagatgatt ttataagact
    27961 ataatctata aatcctggat gagtaaccat caatgttata tctttatcta aatttatata
    28021 tttttcttca aataatttaa caaaagattt atttaacttg taaaaatcat tctgaggata
    28081 tttacttggc atgctaatac atgaatccaa ataataggga caacttggta taccgtattc
    28141 ttttgcagca tcacacattg ctgatattga tacatcttct tctataccat gtatatcaat
    28201 atactcaggc aatttactca tatacaactt gtaagtttca atttgagcac aaacttcttc
    28261 atatacttct tcataattaa aaacctcttt acctgtcagt agttgcgttc ttcttatttt
    28321 gctagaaaca aattttccac cttcatctaa caaattcttt atcttaatat gttctttgca
    28381 acaaggttct cctattacta aattaacatg taaaccaaaa catacatcat cattttttac
    28441 catttctata gcttgtcttg aaaaaggcat attcaccatg attccagcac ttgtaacaat
    28501 accgttctta taagaagata ttatccctag tgtaacacct tctgaatatc ctatatcatc
    28561 tgccctaact actaatttct tcattttatg catctgcctg ctttctcaac tcatcaagtt
    28621 gcatttccat ctcatccttt actttaacaa catccgttcc tatgatgatt tgaactgatg
    28681 tattactcaa tttaattacc cctttggctc ctgttttttt aatattatca tcattcaaaa
    28741 tagtggcatc cttcacttct aaacgtaatc tcgtcataca attattaaga gtcactatat
    28801 tttcataacc accacaattt ttaattatct gtttagccat atatgaaaaa ttaccatgag
    28861 aaagttttaa attcttttct ttttcactaa ctttatcacc tatctctatc tcaacttcac
    28921 gtcctggtgt ttgcacatta tcttttatta ttatatattt aaatgttaca taatataata
    28981 caaagaatac aactcctaca ggtataacta accacgcttt atctccataa ctaaaactta
    29041 agacatagtc aattatactt gctccattaa atgaacccaa gcgagcaccc aataaatata
    29101 gtacaactcc acctagacca gaataaaaag aatgaattat atataacttt ggagatgcaa
    29161 ataaaaacga aaattctatt ggttcagtta ctgttgccat aaaagaagtc aatgctccag
    29221 cggtcattgc accttttact tcttgcttct ttttatcttg tgctgtttta tatatagcat
    29281 aagaaatagc aggtattgaa aacatcataa ttatgtaaaa tcctgtccag aagaatcctg
    29341 catttgggtc tcctgccata aatctaggaa tttctcctct tataacttct cctgttgatg
    29401 ttgtatattc acccatttca aataatatgt aagcatttaa aacttgatgt aacccagttg
    29461 gtattagtgc acggttacag aataaaaaca agaatattcc tattgcacca gctcctccca
    29521 accaaaaacc aaattgattc attgcagctt gtggatatat ccataataaa ctaaatatat
    29581 atgataaaat taaagcaacc actggtgata gtgtaattac acatttttct cctgcaaaga
    29641 agcttaaaaa tgctggaact ttaactttat atgttttatt gtaagtccat gctatacaag
    29701 ctccaacaat tatcccacca aatacacctg catttaatgt atcattcaat aactctaaat
    29761 ttgacttaaa tatcatatat gcaactacgg ctgcaaccat tacacttgtc ttgtctttag
    29821 actttgagta ggcactagct gctcctaata aaaataagta gtccacattt gaaaatacta
    29881 tattagatat attagatata gctggcacat tgagcatatt tggaccaaat aataatccta
    29941 atatacctgc tgctggcatt acagctattg gcaccagcat agtctttcct aaaagtgaca
    30001 gttgttgcat cattttgttt aagaatctct tcatatccaa tcccctttct atttcggttt
    30061 aattttttgt gaaaacgatt tattcacatt ttatatttta aatattatat ttgtttaaac
    30121 aaattgtcaa tattttttat tcattttatt tttatcattt agtttaacat ataaatcctt
    30181 ttaagtgtat tgctatatct agtctatttt tttaattcat tttttttatt ttaaaaatat
    30241 gtttaaccat gtatttgaaa caacccacaa aataattcta ttttgtcctt tttatggttg
    30301 atatgattct attttcacta tacatgtcta aacacatttg atataatttg ttcatatcag
    30361 ttcaataata ttactaaaga cctttaagga gggatagggg tatatggcta aaaataaatt
    30421 ttcagagata tatgaagtat tgaaagagga aatacttgat ggtaaatata caagtaatat
    30481 gatgctgcct actgaacttc aacttattga aaggttttca tgtagcagaa acactgtacg
    30541 tagagcaatt tctcaattaa acacagaagg atatgttcaa agtataaaag gcaagggtgt
    30601 tgtagtgtta gaaaatagtt gttctaatga tttttttcta aatatgcaca atttcaaagg
    30661 tgtagaatcg attgtagaag ataaaaaggt taatactgct actagtgttc tccacttcag
    30721 caaaatctta attgataata agttatctaa aaagacaggt tttaaagttg gtagtgaagt
    30781 atattacctt cacagactaa gatatattga taacatacct aaaattctcg acattaacta
    30841 ttttctatgt agtatagtta aagaccttga tgtttctata gcacaaggct ctatttataa
    30901 atatatagaa gagtgtctag gtacaaaaat tgtttcatca agaaagatat ttaaaattga
    30961 aaaagctaca gaacttgagt taaaaacgct acctctaaat gattataatt gtgttggtgt
    31021 tattaaaaat agtgtatata ctgatgatgg aaagcttttt gaatatactg aatcaaagca
    31081 tacacctgaa acatttgtat tcatggatgt tactcaaaga tattaaaaaa attatgttta
    31141 attaaacata atttttttac gtttagacac tttatcccat actaacctca ctattctata
    31201 tacaattata tgtttataca aataattatt tattgtatat tttagcttta atattagttt
    31261 aacgcttgac aatttgttta aacatattat aattaactta taaattacag gaggtatcaa
    31321 gtatgaaaaa ttggtggaaa aaagcaactg tctatcaaat atatccaaaa agtttcaaag
    31381 atagcaacaa tgatggtata ggtgatataa atggtattat agaaaaatta gattatctgt
    31441 attctttagg tgttgattta ttatggttga ctcctatgta cgtttctcct caaagagata
    31501 atggatatga tatagaagat tattataata tagacccaaa atatgggaca atgagtgact
    31561 ttgaaaaact cttaaaagaa gctcataaaa gagacataaa aataatgatg gatatggtcc
    31621 taaaccatac atctactgaa cataaatggt ttaaagaatc taaaaaaagc aaagacaatc
    31681 catatcgtga ttattatttc tggaaagatg ctaaacctga tggttctgtt cctaataact
    31741 ggatatccag atttagtgga actgcttgga aatatgatga aaccactaac caatattatt
    31801 tacatttatt tgaagaaact caagctgatt taaattggga aaatgaaaag gttcgtgaag
    31861 aatgttacaa aatactagaa ttttgggctg ataaaggtat tgatggtttt cgtttagatg
    31921 tagtcaacct actctccaaa acacctggat tacctgatga ccctataaca ggaccaaaag
    31981 gagatggaag aactcactac gctgatggtc ctcgtataca cgaatattta cataatatga
    32041 atcaaaaagt tttcaaacct aaaaatatag ttactgtagg tgaaatgtca tctactacac
    32101 ctgaagagtg tatcaactat actagagaaa atcgagaaga actatctatg gtttttagct
    32161 tccatcatat gaaaacagat tataaaggag gcgcaaaatg gactaatgaa atgttctctc
    32221 tggataaatt aaaaaaagca caatctgact ttcaatataa aatgtatgaa ggaaaaggat
    32281 ggaatgctct attttactct aatcacgacc aacctagagc tttatctaga tttggagatg
    32341 atagattttt tcgtcaagaa agtgctaaga tgttagccat aacattattt ggtctacagg
    32401 gtactacata catatatcaa ggtgaagaaa taggtatgac aaatgcctac tttactaaaa
    32461 tagatgaata tgatgatgct gaaagtaaga atgcatacta tcatatgatt aaatctggta
    32521 ttgatgaaaa ggaggcattg cttatacttc aacaaaaatc cagagataat gcccgtactc
    32581 ctatgcaatg ggataatact atttataagg ggttttctaa tcataaaccg tggttaaaag
    32641 ttaataatga caatattagt gtagaaaatg agcttaatga ttctcgttca gtattttata
    32701 cttatcaaag attaatcaaa tatcgtaaac aatatgatat ttttacagaa ggtacttata
    32761 gattgttaga tgaaaatcat aaaaatctat ttgtttatga aagagagtat aaaaaccaaa
    32821 atcttcttgt tataagtaat tttactcatg ataatgtagt atataaatta cctgaaactt
    32881 atttgaataa attaaactac acaatacttg aaaccaatta cactagagat ataattgaaa
    32941 ataaaatgga aataaaacct tacgaaagta taatgatata ttataaataa agttatttat
    33001 gtaaaaatcg gtataacacc ctggtgttat accaatttta ctaaattcaa agtaatattt
    33061 atagaatatc tatagattac aataattatt tgacttctac atcattatat ttctttgaat
    33121 atactttagt catatagaaa tatataggaa ctacaattat catcccacct aatccccaaa
    33181 ctatcttcat aggttcatca atacctgctt ttatcagtaa taatatactt ataataactg
    33241 caaatattgg tataattgct cctccaggta ttctaaaatt tacatcttta ccagcatact
    33301 tctttctcaa tattagtaca gctaaacatg taggaatata ttgaataaat ctagatacaa
    33361 cacttatttg agataatgtt gtgaatgaac ccgaccatgc tattagtaaa gttcctatga
    33421 cagatactat aattgctata taaggagctc catttttatt agtttttctt atagacttag
    33481 gcattaatcc ttgttcaacc aaagcagcag cagaccttgg tgtaaatatg gatgatgcaa
    33541 tatttatacc tccaacagat actatagttc ctattgtaat tatataccca cctataggac
    33601 ctaacatctt aatggcagca tctgcaacag gagtagctga ccctgctaag ccattaccca
    33661 acacccctat acttattccc agtataagca tatatattac tgatactaca aacattacca
    33721 ttaccaatgc tttaggtaca tttttcttag ggttctccat atcttttgct gcaacagcta
    33781 aagattcaaa tcctgtaaaa gcataaaata aagttataga tactgctaca aaagctgatg
    33841 acgcactttg acctttttgt attattacaa atggggtaaa attagaaccc tttataaaga
    33901 ataatcctac agctataaat aaaactattg gtaataattt tcctatagtc atgatattat
    33961 ttaagtgctt agattgtttc atccccataa aatttataat cccaagtcct acaactaata
    34021 taccaattat tatattttta gtaagtggtt ctgctgcttg tggccataca gatgatagtg
    34081 ttacagcaaa gaaatttgcc atagtagccc aagcaatcat actaactatc cacttcataa
    34141 atccaacttc aaatccaaca aaatctccaa atgcctcttt tgcataaaga tagggccctc
    34201 catcagtatc aaaccaacta cctgcttcag caaaacataa agcaatagaa acagctaaaa
    34261 gcatacaaaa aactaatact aatatacttg ctggacctat atctgtataa gcatcaccag
    34321 gtaacccgaa tatacctgac cctattattg cattgattcc taatagtaca atactccaaa
    34381 aacctaactt aatgcctttt tccattgtta ttccccccaa agccaactta atatatttgt
    34441 attttttatt tagatgcttc tttaactatt gccataaata tttttttcat attatcataa
    34501 tccctagtca tcatttctgg atgccattgt atccctatta caaactcact tccttccttt
    34561 tctatagctt caattaaacc gtctttagat gtagctgaaa caatatatcc cggtgctact
    34621 ttgttgacaa ctaagtgatg aaaactatta actaaaactt ctttttctcc taatatttca
    34681 tataattttg tcccctcctt tatctgtact gtatgtgtag gtacattaga tagatgttgc
    34741 tgattgtgtt taatataagc accttcaatt aaagataaat cttgatacaa actacctcct
    34801 tcagttacat ttataatttg ttctcctcta catataccta aaactggttt tttcatatct
    34861 aaagcatgtt ttaataattt catatcaaaa ctatctctct ttggtgatat tgcccccaac
    34921 ttattatgag gttcttcttt ccatatgagt ggatttacat cttgtccccc agatagtata
    34981 agcgcatcta tattagatac ctgctccttt ataatctcat catcatatac aatcggtaca
    35041 atatatggta tagctttaca cataactact gattgtatat aatcattatt tacataagcc
    35101 ctttcatagc caggaaacat tcctccttca tcaattagta aattacctga tattcctatt
    35161 attggatatt tctttttagc caatttcttc accccttaac ttatttgtga acaataaaaa
    35221 atgctacttc tcttaaagag caagtagcat accaagattt tatttatttg aacattataa
    35281 tttattttag aaatactttt tttgaatacg agtaatataa ataactttat ttacttatat
    35341 tttattttaa tcctgtttag tttgactttt ttaaattgtt ttaagtacaa ctaataggta
    35401 aaataggcat ttagattttc ctttttatca acctattaaa tactgtttaa tatatttttt
    35461 ccccttctat ttaattcact tccctttcta ccagattcac atattatcaa atccagctct
    35521 tttaacatat taagtattcc ccttacctca ccttctgtta tattgtagcc taattcattt
    35581 attgaattaa caataccatt tctacctata tttttattaa gattattggc attttttaaa
    35641 cactccatta ctaactttac tttctctata gatgttctat cctttaatat acttatttct
    35701 ttactaaaat cttcagttat atttaataag ttatgaggta aatcatttat taaaataaca
    35761 tcctcagaac tcataatgtt aatatacata gcagtatttt ttaattctct tatattacct
    35821 ggccattcat atgcattaat aatgtcttga acatcttgac taattgctct cttccttttc
    35881 ataaaatgct ttaacataac ttctatatct tcttttcgct ctcttaaagg tggtatattt
    35941 atgggcaata catttatcct atagtataaa tctgctctaa atctatctcc ttttatcatt
    36001 tctaataaat ttttatttgt agccgcaatt actctaacat ctatatctat tacattttct
    36061 cctccaaccc tcattacttg attctcttgt aatactctaa gtaatttgct ttgtaagtat
    36121 ataggcatgt ctcctatttc atccaaaaat atagttccat tgttagcctg ttcaaataat
    36181 ccttttttac ctccttttaa agctcctgta aaagcacctt cttcatatcc aaaaagttca
    36241 ctttcaagta aattttctgg catagctgca cagtttatcg ctataaatgg ctgactatgt
    36301 cttggtgatg cattatgaat agactgagcc atgagttctt ttcccgttcc actttctccc
    36361 gttataagaa ttgtataatc agattcagct attttcatac ctaatttctt agttctttcc
    36421 atcttcgttg aaactgtttt tatatcatca aaagtatatc tagcaacttg tcccttttct
    36481 ctaagcttct ttgataggtt ttgctccaat tttcttatgt aagttatttc ttgaatacaa
    36541 aaataatatc cagtaaccct tccaaaactc tctatattat acttatttac gtttaagtac
    36601 ttattattaa attttataac ttcatcatgt gccttactca aactaaaaat ctttttaata
    36661 ttctcattct ctaattcatc tatatttttc cctataatat tactttgaat atctagtata
    36721 tcccctaaac tattattgca aataattatg tttcccttat ctgataccat tgccatacct
    36781 aagtttgata aatttattat agaatttaat tcatctgctt ttatagtaag ttctttatat
    36841 ttagtattta ttcctgaata taaactaatt atttcatctg tatacttaat tagcctttca
    36901 gtaatttctt tatcatccag tttgagtttt gctattattt gaataaacgt tgaactatca
    36961 atatacctat ggcctaaatc aataacttct tctatatatt ttggaactaa acttgcctct
    37021 cctggcgtta tagctatctt aatatttcta tattcttcat tgggattgta tggtataaaa
    37081 ttcagatgat ttattcctat attgtataaa ttagatattg tttccaatac agtatgctta
    37141 ttatcgttta ctactaatac atctattcct tctggcaatg aaaataactt atatatatcc
    37201 ttttgcttta tgctcctatt tatagttatt atctttgaga catcttctac atattttttt
    37261 atttttaata ctctatcatc tatcattacc aatattatgt catcttttat taattccttc
    37321 tctgttattt tatttaaata ataatttttt atcattactt tatctttaaa tatcatttct
    37381 agatttttat ttaagaaaca ttctaattta gacaacccat cagttactac tgctattgtt
    37441 ttcatttgat tactcctttt aaaatatact ctactctttt ataatataca tctgattttc
    37501 actttaacag cacaaaaaat tgtacattta gtatactaac atattccttt attagtttta
    37561 tgtccctaaa tatcatagtt gcaatatcta tcatatttct taaacttcta taaagcaaaa
    37621 attgtgccaa attaataatt ttcttaattt tattctaaag ttcctcaata gtatttacag
    37681 acttagcact tatagcttat aagtctttat attattaatc tatttttata ggttaattgt
    37741 acaaatcata tatcactttt acccattaaa tgcattataa aaaagatgga ataatgattc
    37801 gtcactccac cttttttgta ataaaaaatt gtatatataa acactattta ctactcttct
    37861 aagtacaaac attatctaca tactataacc tttcaccatt agattctatt acttgtttat
    37921 accagtcaaa actctttttc ttaatcctag acaagtctcc acttccatca tcatacttat
    37981 ccacgtatat aaacccatat ctcttggcca tctcaccagt agatgcactt actaaatcta
    38041 tacatcccca cggagtatat ccaaataaat caacaccatc ttctacagct tccttcattt
    38101 gctctatatg tgcttttaaa tactctatac gatagtcatc atttatcact ccattagatt
    38161 cttttttatc ataagctcct aaaccatttt caactaccat cataggtatt ctatatctat
    38221 cataaatttc atttagtgca tatctaagcc cttcagggtc tatttgccat ccccattcac
    38281 tagacttaag atatggattt ttcactccac ctaatatgtt gccacttgta ttatcttctt
    38341 tagaatcact acttacacat gtagacatat aataactaaa tgtataaaaa tctacacaac
    38401 cttcttttaa aatttctaaa tctccttttt ccatttttat ttcaatatta ttttctccaa
    38461 aatatctatt catataagtt ggatattcgc cacgcacttg tacatctcca cagaaataat
    38521 tcattactct actatctctt tgagttttaa ctgcatcatc tggattacaa gtataaggat
    38581 acattgttgc aaatatttgc atacatccaa cttgataatt tgaatctatt tcatgagcta
    38641 acttaacagc cttagcactt gctacaaatt gatgatgtaa cccttgaaaa cgaagttgag
    38701 gtatatctac ttggtttata aaatctgtag tcccttcatt taatattcct tgtgataagt
    38761 agcctcccat aggcattgta gctgaattta tttcattaaa tgtcagccaa tattttactt
    38821 ttcctttata tcttttaaat attgtttcac agtaattcat atacaagtct attacatctc
    38881 ttgaaaccca tccattgtat ttctctgtta ttgcaaatgg tatttcatag tgagatattg
    38941 ttattaatgg ttctatatta tattttaaac attcatctat cacattttca taaaatttaa
    39001 gtcctgcttc atttggttct ttctccattc cagttggaaa tattctagtc catgctattg
    39061 aaaatctaaa taccttaaat cccatttctg caaataaagc tatatcttct ttgtatctgt
    39121 gataaaaatc tattgcttca tgacttggat aaaatgttcc tttttctata actctcgtta
    39181 ttcttttact tactttttga gaacctccag tacacatatc tgaacaagaa ggacctttcc
    39241 catctaaatt ccatgctcct tcacattgat ttgctgctgt tgctccaccc cataaaaaat
    39301 cttttctaag tgccataatt ctacctccaa taatttattt atagtaactt tattttaaaa
    39361 taaaaataca tatatataat tcttaattaa tagaactctc ttttacgcta aaacagttat
    39421 caattcctga ctattattta catgcttttg tgtattttct aaaatatcca cataatcaga
    39481 ataattagta acaactactg gtgtttctaa gcaatatcct tcttttgaaa tactttctat
    39541 gtcaaaagtt attaatatct gacctttttt aatcttatcc ccttgattaa caaaagattt
    39601 gaaatattta ccttccaact gaatagtatt taaccctata tgtatcaata gttcacaacc
    39661 attatcagat ataattccta ttgcatgttt tgttggaaat aaagtcatta cagtcccatc
    39721 aaatggtgcc actacttttc cttctttagg tgttattgct acaccttttc ccaatatacc
    39781 tgttgaaaac gctgcatctt gtatctgctc caaagccttt acttcacctt ttattgggct
    39841 atatactact tctcttttgt ttttttctat aacgccttgc ccattatcat ctttagaaat
    39901 ttctactata gcatcatctt tccaaaaaaa taatgttaaa acaaatccta ctacaatagc
    39961 tactattatt gctattatag ttgcataaac acctttcata tctccagttg ttggatttat
    40021 ataattaggt ataccaaata ttccaagtcc acccattaca taacttttta cattcattat
    40081 ccccattaca gctcctgcca caccaccacc tacacagcta tatatgaatg gtgtttttct
    40141 tggtaaagtt attccatata ttgcaggctc tgttacacca catatccctg aaataactgc
    40201 aggtatacac agctctttta tcttcttatc ttttaattta aagtacattg ctgctactac
    40261 tgctgtttgt gcaaaagatg gtacaaatgc tcccactaat attgtgtcaa accccatagt
    40321 cataaggttt gacatagcta ttggtactaa tccccaatgt agaccaaata ttacaagcac
    40381 ttgccacaat aaaccagtta atgcaccaca taaaattggt gaaaagttaa ataaagctaa
    40441 aaatccattt ccaactatat tagaagcata ggtagctata ggccctataa ccataaatcc
    40501 caatggtaaa gaaactaata aaactgccat tggtacaaag aaattcttta ctacatctgg
    40561 tattatttta gcaaacactt tctccaactt agctgctaat aatactatta aaattactgg
    40621 tactactgtt gaagtgtagt tcattgctat tactggtaat cctaagaaag ttatatatac
    40681 tggtgattca aatattgttc cagtaaatag attgtataaa ggttgacctg tagatgctag
    40741 agtatctaat tgaatacctg aatagcataa aactgcacct ataagcattc caataaatgg
    40801 ttttaatttg aatttcttag cagctgtata tccaagaatt attggcatat actggaacat
    40861 tgcatctcca attgcattta acataatata tgcaccatca gtttctccat acaaacccaa
    40921 tgctgtaaat actgcattaa atccttttat cataccagca gcacacagaa ctcctaatat
    40981 tggttgaaaa actcctgata ttatgtctat aaacttatta agtaatcctt ttggcgcatc
    41041 tgagtctgaa ttatctattg tatctcctcc taatccagcc aaatcgcaca caacttcata
    41101 aacatctgga acatgattac caataactac ttggtattga cctgcacttt tcattacagt
    41161 aactatacca tccatttttt ttagcacatc atcatttgct attgactcat cttttaattt
    41221 aaaacgtagc ctcgttatac agtgagataa actgctaata ttatcctttc ctcctacatt
    41281 cgcaatgatt tcttttgata gttgttcata ctttctcatt ttttctcctc cattttcttt
    41341 ttgaaatatg aaggtttggc caacttaatg tcaccaccca atatatataa acctaattat
    41401 taggatttat atctgactta atacttttac ttaccactct ttcaacgtga atagttaagt
    41461 ataatttttc ctcatctgtt aaatcgtagt tgtatttttt atgtataaat tcatccaact
    41521 tttctatgaa tttataagca tttttatatt taacttttat tacatttaat aaatcatctt
    41581 cattattttc atgtaaatta ttactaacaa ttcgttctgc aaaatacttt aagtgagtaa
    41641 taaatctatg ataataaact gaatcttcat caaatactat tctgtaatgg tatttaacca
    41701 tatttgaaat ttcttgtatt atttgtgtaa tctcatatat agtttttata ctttgatcca
    41761 tttgtgcatt aactatatgc agtgctataa atccagcttc atcttctgat agtctaattt
    41821 caaatttttt ttctatataa tccaatgcct ctaaccctat tttaaattca ctcttataaa
    41881 atctttttat atcccaaagt aatacatttt ttacacttac tccttcacga gctctttcta
    41941 ttgcagtata catatggtct gttaaagaaa tataaatact atcatttaat cttttcccta
    42001 gtttttcttt agcatataaa attatttcat ctgataattc catatatctt ataggaatat
    42061 ctgctattag ttctttaaat ttatcagata tatttgggtc tgaaagtttg aatatcttat
    42121 caattttatc ctttgatata tgctctcctg tacgtttttt ataagctaaa cctctcccca
    42181 taactactat ttccttatga ttttcatcag ttgtaataat tacattatta ttgagtattt
    42241 tttcaataat cattgtgatg ccccctttat tagcattaag ttaaaataaa tcttttttaa
    42301 ttatttagaa tatttaaaat gtaatttatt acataaaaaa aaacctattt attacacatt
    42361 aaatagactc ccatgagtcc tgagtaataa ataggtttag cttgtattta aacaatcact
    42421 atccgtttgt attttatttt aattattatt tattatatgt tagcaaattt attttttagt
    42481 gtcaactttt tctcaatatt ttaattttaa ataaattata taaattgaat cttcaaagta
    42541 ctatttttat caataaacaa aataaaaaag gtattacttt ctttaagtaa tacccattta
    42601 tttttaattt aagtggtttt tcttaatctc atcaagtagt ccatcttcta aataaatttc
    42661 taatgcacat cctaccattg cctttactgc tttgtgaagt ttatcataag cttcctcaga
    42721 atttgcataa tttagaaatt ctttttcatg aacatatacc attggactta tatctaaggc
    42781 tatttctgta tacatagtag ggcaaacttg acttacatta cctatatctg tagaacctac
    42841 tggaccatct ttaccttcta atatatctgt tatacctgcc tcaatcaaat tattctttgt
    42901 tatcttttga agtactttgt tatttattat gttgtcaaaa gaattttcaa agtatctata
    42961 agaaagctta gctcctgtca ttaactctgc accttttgca cagtttataa cttttttagt
    43021 tacttcatct aaataacttc tttcagcagc tcttacgaaa aacttacaag ctgctttttc
    43081 tggaactata tttggagcct ctcctccatt tgaaactata ccatgtattc taacatctga
    43141 ttttacatgt tgtcttagtg catttatccc agcaaaagta agttgtactg catctagtgc
    43201 attgactccc atatgagggt gactagctgc atgtgctgat accccttcaa aactaaactc
    43261 taatgaatct atcgccaaag ccttacagtt tatattatta aaagcttcta tatgcatttg
    43321 ataacttaca tcaacatcat caaaagctcc tgccttaact aaatcacatt ttcctcctgt
    43381 tgtttcttca gctggtgttc ctattagtac aatttttcct ttaaaatttt cttttaactt
    43441 acttaatact atacaagctc ctagtgtaga tgctgcaatc cagttatgtc cacaagcatg
    43501 agctggttct ttattttctc catatccagg aagtgcatca tactcagcta aaaatgctat
    43561 ggtaggtcca tcattatctc ctaattcagc tctaaaagca gtctccatat tacaatatgg
    43621 ataaactgta tcaaatccat attccttcat tttttctact aagtattttg atgatatgta
    43681 ttcttcttcc cctaattcag gattgttaaa tataaaatta caaacttctt caatttcttt
    43741 tccaagttca ttctctatct cataagctct cttctttata ctctctatca taatttacct
    43801 cctactagaa tcctagatat aatcctattg tcaaaaatgc cattacaaga attgttaata
    43861 ctaccattaa aggaattacg aatctaaccc actttgacca aggaatattg gctactgcta
    43921 gtgctcccat taaaacacct gatgtaggtg ttatcaagtt agttacacct ataccaaatt
    43981 gataagctgt aactatcatt tgtcttgata catctactaa gtcgccaagt ggtgcaagta
    44041 caggtattgt aagtgatgca agccctgatg ctgatggtat taaaaatgct attaatcctt
    44101 ctattaagaa agttaaattt ataaatatag tttttggtaa tccatttaat aaatttgctg
    44161 cagaatttaa aattgtatct attataaatc cattttctgc tattattacg attccacgtg
    44221 caaatgctat acacaatcct gctgtaatta aatctcctgc tccacttata aatgaattaa
    44281 ctatctcatc ttgtttaagc cctgatgcta ttcctgatat tatacctata gctgtaaata
    44341 tcattgcaat ttcagttatg taccattcta gtcttaaaac tccatatatc ataatcgcca
    44401 tacctattgt gaaaatggct attaccatgg cttctctttt tgtaaattcc tttatatttg
    44461 tatcactatt aaccattaat tcttgattta gtgatatatc atgtactaaa gattttttag
    44521 ggtctttttt aactttattg gcatacatca taacaaatcc tatagatata gccatatata
    44581 ttataaacat aacaactcta aactctattc cagaacctgg tactatttgt gataatgctt
    44641 gtgctattcc tacagagaaa ggattcgtag tagctgctcc aaatccagct gttgctccta
    44701 taaatactgt ggccactgct gtcaatgaat catatcccat actggtcata agtggaataa
    44761 atatcatata aaaaggtagt gtctcttcac tcattccaaa cgtcgtccca cctagtccaa
    44821 atagaatcat tgaaatcggt attattaaaa attgaaactt cttcatttta tttacagtat
    44881 gagctataac agcttctata gctcctgttt tattaactat cccaaaagct cctccaacta
    44941 ttaaaacaaa tccaactact tcagctgcat ctataaaccc gtttataggt gctgtaaata
    45001 catcagtaat tccttgtgga tttgatggtg ctttttcata agttccagct acaacaactg
    45061 accttccgtc tatatctact ctttcaaaat ttccacttgg cactatccaa gtaagaattg
    45121 ccattaacac tattagcaaa aatattattg taagtgtctg tggcatagta aactttttct
    45181 tttttaaact gctcattttt aatctcccca ctttttaaat taaatatttt attattgcat
    45241 ccctttatcc tattttgtat agatgtaaaa aagctcaaca tggatacatg ttgagctaaa
    45301 tgtgctaaat acaacatacc tgcatcttcc ctttatcttg tttaagatag ctctccatca
    45361 aaattcggga taattttgat gacagtactg tacctattaa atacagtccc agcactagct
    45421 tcggacacta gcacttcggc acttactcct tttatattgc ctcattggtt tccaacctct
    45481 tcaatattaa tcttagtaaa tgcaacctct acctcaatct gtttgttaga tgaggtaatt
    45541 cttattaatt tttattaatt caaagtaatt gtagcataat atgtcgagat tgtaaatatt
    45601 ttcagtataa taattcttac tttatgattt cattccattt ttcacaaata attttttctc
    45661 catataattt aaatactgta gtatatttta aatacacaca aactttccac aaattttaaa
    45721 ttattataat acctttataa taaaaaataa aagctatgta aacttatcaa tttacacagc
    45781 tttatataca ttaatctact ccaagtacag ctttaagctt tgatgctatt gtaataactc
    45841 gtggaccaaa tataacttga actgagtttt tctttttaac aactccactt gcacccaatt
    45901 catttaccca gaagccatct tcatagaatc catcaattga atttagctct accctaagtc
    45961 tagttgcaca gtttgtaaca ttttttatat tattacttcc tcctaaacct tctattatag
    46021 aatgtacaag ctttgtatct ttatcatcta cttcctcttc tactgcattg ttgtatgctg
    46081 ttttaccagc atctacagat tgtctttctc tatattcttt cttactataa agcttaactt
    46141 cttctgaact tcttcctggt gtttttatat caaacttctt gattgcccat gtaaatatta
    46201 tataagtaac tataaaaaat attggtatca aaaataataa tggtaacgca tccacttttt
    46261 gtggttgtaa taaattagga aacatatttc taaatgattc tcccattata gaaacattaa
    46321 acatttctgt aaatacatac gtcaatcctg ccagtggtac atatgctaca aaatacaata
    46381 atggtgcaac aaataggaat gtatactcta ttggttccgt tacccctacc aacatggctg
    46441 ttactattgc tggtatcaat atacttattg cttttttctt attagcatta tcagccaact
    46501 tatacatagc taaagctgct gctggtaata ttgcaaaatg tattaataaa cgacctgttg
    46561 tgaagttacg agttatataa gatagtgtat ctgggtctcc catttgagct aatcttatat
    46621 tattaacacc ctcgtaaatt gctccaccta cttccattgt tccacctact cttgtatatt
    46681 ctatagggaa tgcaattaag tgatgtatac caaatggtaa taatgcttta tccattgctc
    46741 caaaagtaaa tgttcctaat aatccactag aacctatcca attaccaagt ttagataatg
    46801 ccaaccctac tggtggccat ataaagtaca ttgctgtacc caatactatt gatgctataa
    46861 acatcataat cattgaaaat cttggacctg agaaaaaaga taatgcacta ggaagttcta
    46921 tgtcaacaaa tttgttatgt actgcactag ctacaaatcc agctattata gctccaaata
    46981 tactacaatc atatgaaaag attcctaaga aagaaccata taaagaactt cgactatatg
    47041 cttctgcctc tcctaatcca gatgccataa acgcttctgc tgtaactgta tctgaattta
    47101 taccatttgc tgctaacaat gtacttataa ctgtgtgcat tcctataaat aaaacaattc
    47161 ctcctaatgc accccatcct ttttcctttt tagataatcc aaaggctata cctgtaacaa
    47221 agaatattgg taagtatctc attaccataa atcctaaatc gtttattact ttaaagaata
    47281 catttataaa tgacccttct tttattagtc cgctaagccc taatgcagaa acgttttcca
    47341 tatttgcaaa agctgaccct aatcctataa atactcctgc tacaacaagt aatataacag
    47401 gtatcatcat tgctccagaa agtacttgta atttatcctt catagtatta tttcctccat
    47461 ttttattgaa atgccaggct atataatgca tatatatata cttctattgc tttgtataat
    47521 gtatctatac ttatattttc atttgcctga tgtgaatttg gattatcccc ctctattgtg
    47581 ggtccaaatg caactccatt ttttaacact cttgcataag atgcagctcc ttttgtaaaa
    47641 agttcggctt cttttccaaa accttgttta tatgcatttg ataatttatt tattaattca
    47701 ctatctggtt ttacatacag taattttaaa tccttatata tatcaactat taaattttct
    47761 ttttttccaa tttcattaat tctattagtt ataaactccc aacttatacc cttaggatat
    47821 ctaaagtcta ttttcatatt caattcattt ttctctaaga agatactcat tatacatatt
    47881 gtagaaactc ccatatctac atcttcttta tacatcccta atttttttcc atgattatca
    47941 tctgtaaagt atgaatttaa aatatccctt attataacgc ctttatcatt tagtttttta
    48001 attttctcta aatcttttgc taaactaaat gcacagttat atgacctgtg tggatttctc
    48061 gataaagccc tttctcctgt atatcttact aatactttac cttcatctaa ttcttctatc
    48121 tctgtataat aagctaatga ttctactaaa tctttcttat cattagtttt aaaaacagcc
    48181 tcaattttgt cacatacaaa tccatcttct ttgtttgact ttatatcaac taaattgtga
    48241 tttctaaatt tatctttatc tattttcttt cttagattga agtataaaat acctttttct
    48301 ccatttacta ttgggaagtt tccatctgga gaaaatgcaa attttggctg ttcattgaca
    48361 ctaaagtaat gctccataca ttcccaagtc gtctcttcag ctccacccac tatcaatcta
    48421 atctttctct tcggcttttc attaagttct cttaagaaaa gtaatgcata taaaatccct
    48481 atcaaaggtc ctttgttgtc attaactcct ctaccataaa gatagttatc tttttgacat
    48541 actttaaaag gtttactctt ccaaagttct ttattatata ttggcaccac atctatatga
    48601 ttaagcaccc ctactacttc ttctccttct ccatattcta tatgaattgc atatccatca
    48661 aagtctttta caacaaaatc tttgtctttt gctatttgaa taagcttatc gaaggcattt
    48721 cttatttcta tcccaaatgg ttgatttata tctgttgtac tttcatctct aattgaaggt
    48781 atttcaacta aacttctaat atcatttatg atattttctt gattggattt taaataatca
    48841 ataattagtt ctttgtttac tatcatctta attatttatc acccctttat attaatttta
    48901 caaagcattt cagttcatat ctacattata aattttgaat attatttttt caatatttca
    48961 aatacttctc taagttacca taatgatacg gtaaacaatc aacagattat gacaaaatca
    49021 tactatacct attactaata ttttaactta tctattacgt cttttttaat gtaagtttat
    49081 tccaaaaata cataaaaaaa gatgtagcct tagctacatc ttcttatatc tattcaactt
    49141 ccattattat gctattaata tctacatttc caggttctat cctatctttt actgttctct
    49201 taacagaatc agcatcatcc attataacta caggagttat tatatttaaa tcatttgacg
    49261 ttataaaatc taaatctact tccattatta aatctttagc ttttacctta tctcctgcct
    49321 ttaccttaac atcaaacggc tttccttcta gtgacattgt ctctaaacct acatgtatta
    49381 atatatttgt attatcatta ctcttaatca ttattgcatg ctttgtatca aatacattga
    49441 ctatttcacc atcaacagga gaatataact taccttcact tggtattatt gctactccat
    49501 ctcccatcat cttttgggca aaaactgcat ctggaacatc ttctaagttc actaaatttc
    49561 ccttaaaagg gctatatatt ttctcaatct tattttcttt tttcttaaat atattaaaca
    49621 taacacactc tccacattat aaaattttat gataatattt ttcttaaagc ttttgctaca
    49681 ccatcattta cattagtatc agttacaaaa tctgccatag attttacttc atctgtagca
    49741 tttcccatag caataccaag acctgcaaat ttaatcatgg atatatcatt ttcattatca
    49801 cctatacaaa taacttcttt gctatctata cctaatatat cacaaaattt tttaactgca
    49861 ataccttttg aagtaccttt actcataact tcaaagttta tctttccaga acttactact
    49921 tctaaatctt cttgtttttt aaattcttcc tttgcttctc tcaaagaatc tacgttttct
    49981 gagaaaagta tacattttaa aatttcatta tcagcttttt ctaataaagt ttcacaactt
    50041 gcattttcta ttatttcaat tctgtcttct tctttatttt tactatttat taatctatat
    50101 ggatgttctt cacctatttc attgttggat ataactattt tacagccttt aaaatgtttg
    50161 tttaaattat acttttcacc tattgtatag attttcttta gagcttcttt gctcaaaacc
    50221 ttcttatact catatcccag taatttaaaa tatgtcccat tagttgatat aatatatata
    50281 tcatccccta aaacacttgt aaaatatttc atagcattat aaggtcttcc cgtaactaag
    50341 gctaccttga tacccttttc catggcttct tttagtgctt taatattttc ttctgatact
    50401 tcaaatcctt tacccatcaa agtaccatcc atatcagtac aaattaattt atatcgcatt
    50461 tgtatcacat cctagtaaga tttttctaat atttcaacaa ggtcttcttt ctttccaaaa
    50521 ggtaaaaatc ctaaaactgg acagcttgtt atttgttcta attgttcctt tgttacatta
    50581 aactcagaca tttttgtctt aagccccact ctctttaaaa aactttctat ttcatttcct
    50641 aaagttttag ctgcttcatc atcttccact ttttctaatt cttggtttag tattctcgct
    50701 actttggcaa atttctctat attatcctta tagaactttt ctgcaaattt tgggaacact
    50761 atagccaaag cctcaccatg aggaatatgt gtaactcctc ctattatttc acttagtgga
    50821 tgtggtgctg ctgctccagc atttgccagt gaacttcctc ctaatgtatc tgctactgct
    50881 aactttgttc tgtattctat attattttta tcttccatta ctttaggtag atagttaatg
    50941 actaattcca tagatttttc agacattatc tctgtaaaat cattagcatt tggatttata
    51001 taactttcaa atgcatgagt aaaagcatca aatgctgttg atgcagtaat tctctctggc
    51061 agagttaaca taagctctgg gtcaactata cattcttttg agaaacaatt ttgatgaaat
    51121 attgtatttt tctcgcttcc tctcgatatt actgctgctt gtgtaacttg gcttcctgtt
    51181 ccagaagtag ttgatacagc tattaatggt aattcttttt cacttacctt atcgtatatt
    51241 ttaaatggat ttgtatatgt actaaacata taatcccaat ctattttttc taaaccaaaa
    51301 gtcaatgcta atacttttgc agtatctata cttgaacctc ctccaacagc taaaacaacg
    51361 tctacagatt cttttttagc taattcaaac ccttcgttta ttaattctac agttgggttt
    51421 ggctctactc tctgaacctc ccatgactaa agtcacaggg ttcctgcttc acagaatttt
    51481 gcatactaaa atatgtctta ctaattctcc acaggccatc cccgtagttc ctacggttct
    51541 tacataagtt atttattttg aattgttatt agtcttaatc cttcttttaa tatatttata
    51601 cttgcattta tatctctatc gtgagtttca ttacaactag gacaaatcca ttctcttata
    51661 tttaagtcct tcacttcctc gtttttatac ccacatttat tgcatatttg tgaacttgca
    51721 aagaatttac ctacctttac aatttgtcta ccatgccaat tagccttgta ttctaattga
    51781 cgaataaatt ctgaccatga tacatctgaa attgagcgag atagtttacg atttttaatc
    51841 atattcttta cttgtaaatc ttctatgcaa ataatatcat tctcttttat taatttagta
    51901 gataattttt gcagaaaatc atttctttga ttagctatat gttcttgaag tcttgcaact
    51961 tttaatcttg ctttatttct atttaaacta ccgattgttt ttctcgataa ttctctttgt
    52021 aatttagcaa gtttgtttaa tgacttctta agatacttag gattttctat aaattctcca
    52081 caacttgaga tacaaaattc tttaattccc aaatctaaac ctatatgatt attagtattt
    52141 tcaaatgctt caatatctac atcagtacag cataatgata cataatatct accacttggt
    52201 tctttagata tagtagcatt aagtattcta ccttgaggta cttgtttatc ccttatctta
    52261 accataccaa gtttaggtag ttttatatat tgaccacaat acataatgtt tccatttgta
    52321 aagttagttt tatatgaaaa tctattaatt tt
//
LOCUS       DAYATN010000013        50568 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.013, whole genome shotgun sequence.
ACCESSION   DAYATN010000013 DAYATN010000000
VERSION     DAYATN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.013"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(88..2277)
                     /locus_tag="ACGI8A_000739"
     CDS             complement(88..2277)
                     /locus_tag="ACGI8A_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HFH0178750.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
     gene            2608..3339
                     /locus_tag="ACGI8A_000740"
     CDS             2608..3339
                     /locus_tag="ACGI8A_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFH0178751.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            3419..4054
                     /locus_tag="ACGI8A_000741"
     CDS             3419..4054
                     /locus_tag="ACGI8A_000741"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFH0178752.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(4305..4898)
                     /locus_tag="ACGI8A_000742"
     CDS             complement(4305..4898)
                     /locus_tag="ACGI8A_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HFH0178753.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(4969..5832)
                     /locus_tag="ACGI8A_000743"
     CDS             complement(4969..5832)
                     /locus_tag="ACGI8A_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFH0178754.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            5995..7164
                     /locus_tag="ACGI8A_000744"
     CDS             5995..7164
                     /locus_tag="ACGI8A_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0178755.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNSNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            7265..7741
                     /locus_tag="ACGI8A_000745"
     CDS             7265..7741
                     /locus_tag="ACGI8A_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFH0178756.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSIPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            8306..9100
                     /locus_tag="ACGI8A_000746"
     CDS             8306..9100
                     /locus_tag="ACGI8A_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HFH0178757.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            9124..9567
                     /locus_tag="ACGI8A_000747"
     CDS             9124..9567
                     /locus_tag="ACGI8A_000747"
                     /inference="COORDINATES: protein motif:HMM:NF025547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HFH0178758.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(9656..10942)
                     /locus_tag="ACGI8A_000748"
     CDS             complement(9656..10942)
                     /locus_tag="ACGI8A_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFH0178759.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(11160..12050)
                     /locus_tag="ACGI8A_000749"
     CDS             complement(11160..12050)
                     /locus_tag="ACGI8A_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFH0178760.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEIIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            12342..12686
                     /locus_tag="ACGI8A_000750"
     CDS             12342..12686
                     /locus_tag="ACGI8A_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFH0178761.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            complement(12845..14941)
                     /locus_tag="ACGI8A_000751"
     CDS             complement(12845..14941)
                     /locus_tag="ACGI8A_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HFH0178762.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNGLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(14954..16114)
                     /locus_tag="ACGI8A_000752"
     CDS             complement(14954..16114)
                     /locus_tag="ACGI8A_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFH0178763.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVATILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            complement(16133..17200)
                     /locus_tag="ACGI8A_000753"
     CDS             complement(16133..17200)
                     /locus_tag="ACGI8A_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HFH0178764.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            17552..18118
                     /locus_tag="ACGI8A_000754"
     CDS             17552..18118
                     /locus_tag="ACGI8A_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFH0178765.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(18332..19318)
                     /locus_tag="ACGI8A_000755"
     CDS             complement(18332..19318)
                     /locus_tag="ACGI8A_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFH0178766.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGTRAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISEGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(19348..20043)
                     /gene="pxpB"
                     /locus_tag="ACGI8A_000756"
     CDS             complement(19348..20043)
                     /gene="pxpB"
                     /locus_tag="ACGI8A_000756"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFH0178767.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(20063..21283)
                     /locus_tag="ACGI8A_000757"
     CDS             complement(20063..21283)
                     /locus_tag="ACGI8A_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFH0178768.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(21312..22103)
                     /gene="pxpA"
                     /locus_tag="ACGI8A_000758"
     CDS             complement(21312..22103)
                     /gene="pxpA"
                     /locus_tag="ACGI8A_000758"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFH0178769.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            22691..23320
                     /locus_tag="ACGI8A_000759"
     CDS             22691..23320
                     /locus_tag="ACGI8A_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HFH0178770.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(23434..24948)
                     /gene="glpK"
                     /locus_tag="ACGI8A_000760"
     CDS             complement(23434..24948)
                     /gene="glpK"
                     /locus_tag="ACGI8A_000760"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFH0178771.1"
                     /translation="MEKKYVMALDQGITSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            25192..25734
                     /locus_tag="ACGI8A_000761"
     CDS             25192..25734
                     /locus_tag="ACGI8A_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0178772.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            25739..28240
                     /locus_tag="ACGI8A_000762"
     CDS             25739..28240
                     /locus_tag="ACGI8A_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0178773.1"
                     /translation="MFTLNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPAR
                     TIIQNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLI
                     CGLSSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIA
                     NILGSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGT
                     LMLICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILS
                     FEYFLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIA
                     APLSGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFT
                     MGTPLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDL
                     VGKPSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKS
                     LLNKVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGE
                     NKLNSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKE
                     LTTAYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVN
                     EQKESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRTMESQKKELQSKLNNSISQ
                     KKDMEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTL
                     QSTLNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            28661..29059
                     /locus_tag="ACGI8A_000763"
     CDS             28661..29059
                     /locus_tag="ACGI8A_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0178774.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(29276..29485)
                     /locus_tag="ACGI8A_000764"
     CDS             complement(29276..29485)
                     /locus_tag="ACGI8A_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178775.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(29517..29726)
                     /locus_tag="ACGI8A_000765"
     CDS             complement(29517..29726)
                     /locus_tag="ACGI8A_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFH0178776.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            30025..30444
                     /locus_tag="ACGI8A_000766"
     CDS             30025..30444
                     /locus_tag="ACGI8A_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0178777.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            30672..31127
                     /locus_tag="ACGI8A_000767"
     CDS             30672..31127
                     /locus_tag="ACGI8A_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178778.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(31348..31608)
                     /locus_tag="ACGI8A_000768"
     CDS             complement(31348..31608)
                     /locus_tag="ACGI8A_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178779.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(31871..32224)
                     /locus_tag="ACGI8A_000769"
     CDS             complement(31871..32224)
                     /locus_tag="ACGI8A_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1375 family protein"
                     /protein_id="HFH0178780.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(32241..37562)
                     /locus_tag="ACGI8A_000770"
     CDS             complement(32241..37562)
                     /locus_tag="ACGI8A_000770"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178781.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFIKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGNGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            complement(37579..38562)
                     /locus_tag="ACGI8A_000771"
     CDS             complement(37579..38562)
                     /locus_tag="ACGI8A_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFH0178782.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKVD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(38563..39261)
                     /locus_tag="ACGI8A_000772"
     CDS             complement(38563..39261)
                     /locus_tag="ACGI8A_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFH0178783.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(39258..40310)
                     /locus_tag="ACGI8A_000773"
     CDS             complement(39258..40310)
                     /locus_tag="ACGI8A_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFH0178784.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(40303..40731)
                     /locus_tag="ACGI8A_000774"
     CDS             complement(40303..40731)
                     /locus_tag="ACGI8A_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFH0178785.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(40731..41057)
                     /locus_tag="ACGI8A_000775"
     CDS             complement(40731..41057)
                     /locus_tag="ACGI8A_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HFH0178786.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(41073..42602)
                     /locus_tag="ACGI8A_000776"
     CDS             complement(41073..42602)
                     /locus_tag="ACGI8A_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFH0178787.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLNVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(42884..43306)
                     /locus_tag="ACGI8A_000777"
     CDS             complement(42884..43306)
                     /locus_tag="ACGI8A_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFH0178788.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(43306..45759)
                     /locus_tag="ACGI8A_000778"
     CDS             complement(43306..45759)
                     /locus_tag="ACGI8A_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178789.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MILAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     VWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(45759..45926)
                     /locus_tag="ACGI8A_000779"
     CDS             complement(45759..45926)
                     /locus_tag="ACGI8A_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178790.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(45953..46399)
                     /locus_tag="ACGI8A_000780"
     CDS             complement(45953..46399)
                     /locus_tag="ACGI8A_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0178791.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(46525..46953)
                     /locus_tag="ACGI8A_000781"
     CDS             complement(46525..46953)
                     /locus_tag="ACGI8A_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFH0178792.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYIGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(46967..48031)
                     /locus_tag="ACGI8A_000782"
     CDS             complement(46967..48031)
                     /locus_tag="ACGI8A_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFH0178793.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(48036..48479)
                     /locus_tag="ACGI8A_000783"
     CDS             complement(48036..48479)
                     /locus_tag="ACGI8A_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFH0178794.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(48728..49168)
                     /locus_tag="ACGI8A_000784"
     CDS             complement(48728..49168)
                     /locus_tag="ACGI8A_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178795.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(49273..49470)
                     /locus_tag="ACGI8A_000785"
     CDS             complement(49273..49470)
                     /locus_tag="ACGI8A_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0178796.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            49639..49959
                     /locus_tag="ACGI8A_000786"
     CDS             49639..49959
                     /locus_tag="ACGI8A_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0178797.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            50041..50547
                     /locus_tag="ACGI8A_000787"
     CDS             50041..50547
                     /locus_tag="ACGI8A_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFH0178798.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
BASE COUNT        16752 a         8477 c         5702 g        19637 t
ORIGIN      
        1 tttttatgca aattgattat tttataaaaa taagtgtcat aaagaaatgt ctgaaatcac
       61 tttatgacac ctatcataat aaaaaaatta ttctggtaat gctcttttat ttacttttcc
      121 gattggtgta actggtatct tatctatcct cattattatt gttggaatca tatatggagg
      181 tagatgctca tttaaatatt ctctaatagt tctttcattt atcttaccac tatcttcctc
      241 atcaattaca taatatgcac atatataaat atctccatca tcatttgttc tcgatataca
      301 aacaacactt ttaatttctg taatattatt tattatattt tcaatctcat atagctctat
      361 cctaaactca tcaattttta cttgattgtc catccttcca atataatcta aatttccatc
      421 atctaaccat cttactaaat caccagttct atacattaac ttattatgtt cacaagtttt
      481 gtaaggattc tctacaaata cttgctctgt tagccctttt ttatttaaat atcctcttga
      541 tatttgaatg cctgatagac atagctctcc tggtgctcca attggacata gtttgttctt
      601 tttatctaat acatatgcat aagtattaaa tataggtttt cctattggta tttttccata
      661 ttctctgtcc acatgaaaag aagtcgatac aacagtaaat tcggttggtc catactcatt
      721 atatagttca taattacgct ttctatatcc ttttaatctt tctcctccag tctgtacaac
      781 tctaagtgaa ttattattgc aattttgtat aaataacctt gcaaatgctg ttggcaatgc
      841 tagtatagtt atgtcatttt cctcacagta ttcatttact gtatattcat caaatttagc
      901 ttcttcaggc ataatatcta ttgatgcacc tacaagcaaa taagggaata gttgtttcat
      961 tacagcatca aaactgaaac ttaagtatat accacatata tcattttctg tagcgcttat
     1021 agattttacg ctataataac acatatttac taaactctta tgttctatag ctatggtctt
     1081 tggcctacca gttgaccctg aagtagatat cgtatatgca caatttgatg atgttatttt
     1141 ttcagttaat tcatatccaa acttaaacat ttcttcatca tttatatcga gtactatggt
     1201 attctcattt acaaccttac ttttgagttc atctgtagtt atcactactt tcgcattact
     1261 atcttcaaga aggtaattca atctttcttc tggattactt gtgtctattg gaaaaaatgc
     1321 tccccctgat ttcataatcc ctattgctgt aataattatg ttaatatctc ttggtaacat
     1381 tacagctact atgtcttcac tttccactcc tatatgattt aaataacctg caagtttatc
     1441 agttacttca tccaattctt tatatgttaa ctttctattt tcatccctag cagctatatt
     1501 actaggtgtt accttaactt gctccctaaa taaatcaaca taacttttat catagggtac
     1561 ttcatattga ttattattaa agtcttccaa taatactttt tcttcttcct tacatacaaa
     1621 ttctaaatct tctattaaaa tatcattatc ttctattatt tgctctatta tataatcaaa
     1681 ttgatttaaa attcttctaa tagtttctct tttatatata gatggtttat actttatttt
     1741 tattttaagt gccctcttag tatatactat tacagataaa tcatatcctt ttttttctat
     1801 gtcataatca tatgataaat caaaactttt agctataaat attgtgttta taagcttagt
     1861 atttagatga tttttattct ctatatctga taacaaatag tatccatact ctaaagacct
     1921 gctccactgc ctttttagct tccttacaac atctataaat ttttcacttt gtctaactct
     1981 tactcttatt ggaattatat tcattgtttt accaaaaact acatcatctt catatatata
     2041 cttttgaagt ataaggccat atatagtttc tatgatagag tctatgctaa tattgttcac
     2101 tttagaatat tgaattaacc tttcatgttt ctcaatattc atcttaaaaa cctcatctac
     2161 tacttcatca gaacaatctt tataaaaaaa atcatttgga atttttgcta tattcgtata
     2221 atcatctaac aaatccatcc aataattcat agctcgttct aaattaactt catacatatc
     2281 aaaattcccc cttactacta agtctataga aaaaattctt ttggaatttt attttcacaa
     2341 tatataaata tattaataat acatatgcaa gctctaaaaa catgagcttg cataaaatta
     2401 cacttattat gactactaaa ttcgtaatat ttacaatgtt taaaatatat tataacacat
     2461 atatgatttt atttttctac atttatataa tatataaatt acaaatagtt atttaactaa
     2521 tatataacaa taccttttgg aatatgatat attatattaa ttaaattttt ataagataag
     2581 ttatacttat aggagggaac catataaatg aatatttatg ctcatagggg gtttagtgga
     2641 aagtatccag aaaatactat tctagctttt aaaaaatgtt tggatatgga tatatatgga
     2701 atagaacttg atgtccacag aactaaggat ggaaaaattg tagttattca tgatgaaaag
     2761 gttgatagaa cttttaatgg acatggtttt gtaaaagact ttactttaag aaaattaaaa
     2821 actctaaact catcttttga aggatatcaa agcaataaag aatgtaaaat tccaacttta
     2881 gaagaagtat taattctaat ctcacctaca gaccttactt taaatatcga actaaaaact
     2941 gacaaaataa actatcccaa tatagaaaaa ggcgttttag agcttatttt aaagtataat
     3001 atgaaaaata gagttcttat atctagtttc aacagcaata gcttaaaaaa ctttcataaa
     3061 ttagacccta gtgtaaaaac aggattattg tgttatttac ctataaacaa tgtagtcaat
     3121 tttgctaaat ttctgggtaa tagttatctt catccacctc tagttttagt aaatgaatct
     3181 ctaattgaac tttgtcacaa aaatctactt ggtgtcaatg tatatactgt aaatgaagag
     3241 gatgatatac tacactgtct aaaactcaat gtagatggta tttttactaa ctatccagat
     3301 attgcttcaa atcttttaca ctcaaaacaa tacagctaat tatacaatag tttgtactcc
     3361 ttgactttta tataattaat atgtacaatt ttattaaatt tatcttggag gtataactat
     3421 ggataactta ttaaatgaac ttgaagcttt aaagagtaat ttagataatc taccaataga
     3481 aaaaatagaa aattatgatt tgagtaaaac tgctttattt ataattgatg ttaataatgg
     3541 ctttgcaaga caaggtgcat tatactctcc tagagtagag tctttaataa agccaattga
     3601 aatgtttaca aaaaaaatat caaacaaatt aaatagagta atagcattta ctgattctca
     3661 tactcctaaa tcaatagaac ttttgagtta tcctgttcac tgtttggaaa atgatgtaga
     3721 aagtgaattg gttgatgaac ttaagtcgat agaaaaccta caaattcttc ctaaaaattc
     3781 tactaatggt ttttttgcat tagaaaattt ggattttgat aatatagata atataattat
     3841 agttggcgat tgcacagata tatgtatata tcaatttgca ataactctaa aatcatattt
     3901 taatcaacat aatattgaaa aaaatatagt tgtacctatg aatttagtag atacttatga
     3961 tattcctaac gttcaccctg ctgagatttt aaacttagta tttttcaaca gtatgattca
     4021 aaatggagtt aatgtattaa aagagattcg ttaagcttct aatactttac atcccatata
     4081 atacaattat cagatattta tttttaataa aatttctaat agaaaaacac agttttagtg
     4141 agaagattac aatttaaaac actatctatt attactaatc ttcacaatat aaaaaatcgc
     4201 agtttagata ttccttatac aaatttataa ggaataataa attgcgattt ttatttttaa
     4261 gtagtacaat taattgatac tacttcagta tttaaattca ttttttaata atcatactta
     4321 ttcgtagttt cgcacattac tacctttatt tttttattaa acatattctt atattctaat
     4381 aaaaactcat ttagttctga atccataaga tttatttttc tatcactaca aagcattatc
     4441 ttttcttggt cgattattac attaatatca tttaagtcat tttttataaa tgttgttata
     4501 caactctttg gttggatttt ttctccatat ttctcataaa atcctgcact cttcttaaat
     4561 gactctaaat cttctgtaga tactttgctg gaagtattat tactcataaa acctgccgta
     4621 gaagggtcga ttccaaagtt tctaagttgt ttttctactt cttcagtgtc cataccatat
     4681 ctctccataa attgcttttg tattttcata aatttttctt gagatatgtt gttatctctc
     4741 atataatcga atatttttct tgaaaactca gccatagaca tctttccatc cataacagaa
     4801 aaatataagt tatatatgta ttgctcaaat ttatctacat cagaatcttt aactttgttc
     4861 tttagttcgt taaagtctat tattctatca tcagacattt ttcatcctcc tagctaaatt
     4921 tttacttgta taagattata ccatatttct attatattta tatacacact atttttttaa
     4981 tttaacgatt ttttctgtag catctaattc gcacattctg cccaaaggaa tagttatcat
     5041 tgggatacaa tgtcctgact gaagattata tatagaaggt ttattgacct tatttgcaaa
     5101 ttctattagt aattccatca aatcattttc ttcaatacaa tccttaaaat ctccaaaaat
     5161 tataccatta cattcctcaa gttttcctga agaatatagt tgtgttaaca ttctatccag
     5221 tttatattga ggttctccaa tttcctcaat aaacaaaatt ttatcttttg catctatttc
     5281 atattttgtt cccattgtgg caattattaa ggataaattt cctcccatta ttattccctc
     5341 tgctttacct tcacatacag tataaatctt ttcttctttt ggattttcta aatataattc
     5401 atctttaaat tctaaagcat ttaccaatga tttataggta aatttatccc atttacctac
     5461 atttgctgcc attattccat gatatggtaa aagaccacaa tttttataga aagctaaatt
     5521 taaacctgtt atatcagaaa aacctataaa tggttttgga ttttgcttta ttatatcata
     5581 atttatttta tccaatattc ttgttgttcc ataacctcct cttaagcaca taattatatc
     5641 tacatcttta tctaaaaaca tatcttctat atcccttgct ctaatatcat cattaccagc
     5701 taaataacct ttataattta aactacaact ttcagaaaac ttaacttcat aaccataact
     5761 ttctaaatta aactttattt cctccaaact tgtatttctt aatggcccag atggcgcaat
     5821 tattcctaac atttaaacac ctcttcttat aaaatgatat attcttctcc atactaaata
     5881 tctctaatat aattataaga tattttattt gtacttgata cataataatg atttacataa
     5941 aataatataa ttaatgaaga aaaataaatt tttaatatag ttaggatgtg ttttatgctt
     6001 aatataaaaa ctctttgtca tgagataaat gattgggtta taaatataag aagagattta
     6061 cataagactc cagaacttgg attagaagaa tttcaaacta agaaaaaaat tattaaatac
     6121 ttaaatgaaa taggtataaa ttatattgaa tataagaatc atacagggat aactgcatac
     6181 attaacgtga gtccaaactt tgaaacagtt gctataagag ctgatataga tgccttacct
     6241 ataacagaag aacttaatta ttcttataaa tctattaata ttggcaaaat gcatgcatgt
     6301 ggtcatgatg cacacactgc tattttactg ggtacttgta atatactatt taaattaaag
     6361 gattacttaa atgtaaatgt aaaattcttt tttcaacctg ccgaggaaac tattggtggg
     6421 gctcaactta tgatagaaga tggatgtctt gaaaacccaa atgtaaaata tatatttgga
     6481 cttcatgtaa attccaatat caacaaaaat ctaattgaat taaaatacaa cactctaaat
     6541 gcttccactg atacacttca attaactgta catggttcaa aatgtcatgg agcttatcca
     6601 catcaaggta ttgatgctat tgttatatct gctcatatta ttacagctct acagacaatc
     6661 gttagtcgaa acacaaatcc aactgattct gtggttattt ctctaggaaa gattgaaggt
     6721 ggaatcaagg aaaatatagt ttgtgataaa gtggttataa ggggtacttt gagaaccttg
     6781 actcctgaaa ctagagagtt ttcaaaaaaa cgaattagag aaatttgcga ttttacttgt
     6841 aaaacatttg gaggaagtat ttctgttgaa attgaggaag gttatccagc tcttatcaat
     6901 tcaaaccatc tagtagatta cgtcaaacaa aatgctgtag aattgtttgg agaagaaaat
     6961 attatcctta aagactcgcc cactcttggg gctgaagact tttcgtattt tctaagacac
     7021 tgtgaaggag ctttttacca ccttggttgt gcaaatcgtg aaaaaaacat tacttcccct
     7081 ttgcatacct ctacttttga tattgacgaa gattgcctta ttactggtgt catgctccat
     7141 gtaaaaaatg tattatcatt ctaactatta atatttttat ttattatttt atattgaccc
     7201 tcacatgatg tcatgttata ccctaaaatt acaaatgata aattaaataa ttggaggtaa
     7261 tattatgaaa gaaatattaa taaatcctat aggtaaaata tgtattcaag gcgaagaggt
     7321 ctttattaaa ttagacaaaa agtatgtacc tgcattaaat gaactcgacg atttcagtca
     7381 tttaaatgta ttttggtggg ctgatgggtt tgataatcct gaactaagaa gtattttaga
     7441 aaccccaaaa ccttataaaa gtggaccaga cgttatagga atatttgcaa caaggtcacc
     7501 tataagaccc aatccaatag cattgactgc tgtacaaatt atcaatatcg accatgaaaa
     7561 tggtataata aaaataccat atattgatgc taatgataat agtcctgtta tagacttaaa
     7621 gccatatact ccaagcgttg atagggttga aaatccgtcc attcctaaat ggtgtagtca
     7681 ctggcctaag agtgtagaaa aatcaggaga atttaattgg gaagatgaaa ttgaatttta
     7741 aattactttt ttcttgattt cattaacttg ttttccacat ttattttatt tacacttacc
     7801 aagattttat gtcacatata catataatat taggctgtat tatataatac agcctaatat
     7861 tatataataa atttctattt ttcccttaaa tcattcacat tctttctaag ctacataaga
     7921 tttttctatt atttcttttt gagtttaatt tagcattatg ttgaggaaaa aacatttcat
     7981 gcattttaat aaattaaatc tttcttttat tctgcgattt tataataatc aatttaactt
     8041 aaaaataata cctaactatt ttaactatgt taaactagta aatgtaacca cttatatgtt
     8101 acaattttat ataaacatca gatataaaaa ctatataaat aaatgacaca tagtgttata
     8161 ttttttaata ccaatttagt tatttttcag ttacagattt aataataatt aatagtaaga
     8221 cataatattt ttatgccctt tattgattat gtattttctg ttatctgtta ttatcatcaa
     8281 taataaacat aagggggtat atattatgaa attgtattta agtgctgata tagaaggtac
     8341 ttgtggtatt gtaaattggg atgaaacaaa acttgaatgt gaattttcgc aacactatag
     8401 aacacaaatg agtaaagaga taacagcagc atgtgatgga gctactcaat ctggtgtaca
     8461 tgaaatactt gttaaagatg cacatgacag tggtagaaat attaatccaa atattttacc
     8521 tgaaaacgtc aaaatactta gaggatggac caaagaccca ctagttatga tggctggaat
     8581 tgatgaaagc tttgatgcat gtatattcac cggctatcac tctggagcta cttgtaatgg
     8641 taatccactt tcccatacta tggatattga ttatgactat tttaaaataa atgatgaaat
     8701 agcatctgaa tttactataa atgcttatat tgctgcatat tataatgttc ctgtagcatt
     8761 tttaagtgga gatgaaatgc tttgtgagag tgcaaaaaaa ctaaatccaa atatagtcac
     8821 tgttcctgtg tcaaaaggaa ttggaaatgg ttcaatttca atccatccaa accttgcact
     8881 aaaaaaaatt gaaaaaggtg tcaaaaaagc tttatctgga gatttatcta ggcacttaat
     8941 aaaattgcca gataaattta agatagaaat aaaatttaga gaacattata aagcttttaa
     9001 ggcctctttt tatccaaata tgaaaaaaat agattctcaa acagtactat ttgaaacaga
     9061 ggactattat gaatttttaa gaatgttatt atttatataa atttcatatc ataaattaag
     9121 gagttgatta agataagtaa taaaagaagg attcctttag gcgaatttat ttctgtattt
     9181 ttattttttg ctgttttttt tggtataact ttttgtattt tcacggttat aggaattggc
     9241 ttcttatcat tcttaggatt tgaatataaa tctcttggag ctgtattaat tttcttttta
     9301 atatattttt gtataaccac tccaattgat tttctatgca ctactatttt agatatattc
     9361 agatatgtaa acaaattacc atactcaata tataaacttt gcgaatttat aattgatttt
     9421 atactaactt ttctagctat gaacataatt gataccttta tggattcagt aacgattcct
     9481 ttaagtacag aaatcttatt cgctttactt tcttatcttt tatcagaatg tatggatttt
     9541 tttgataggg ataagaaaaa accttaataa aaaagataga ttccttaata gaacacatat
     9601 tttcgtattt ctattattta atctatcttt attttattta ataaattttt attaattaaa
     9661 atatagtttc ttctatctcc ttagtcattt gcttagcact atcaaaaaat tcttctatat
     9721 acactacttc atcactgacc ctagaatatt ctgctgcaaa tgccattaca tgactttgta
     9781 tagcttcttt tgctgctact ctaccctttt ctagttcttt atttgaaact aatttaataa
     9841 attcagctat aagatttcta tcatattcct ctaccatatt ctgttgattt gatggattca
     9901 taactatttc ttcgtttttt ccaaattctt taattcttat ctcattttta tttaaatccc
     9961 caccaacttc accatggcta aacattaact ttatagttct tccattttct ttagtaaatg
    10021 cacataaatt aaaagtagcc gtcactccat tttcaaactg tagtatattt accatattgt
    10081 ctacaacatt attatcacat ctgtaaacac atcttccata tggacttgtc ttaagtatat
    10141 ttaatagatt ctcttcagtt ggatttatgt gaacagctct atttatactt ctatcttttt
    10201 ctaaataaat tctctttgct gaatatggac attttttttc aactaaacac tgaagacaat
    10261 tttgtgccat ctctccacta gcattttggt tattaagatg ctttaagctt ccaaatgcag
    10321 atattttttt gcatctactt cctactaaat ataacaaaat atccatgtca tgacaactct
    10381 ttgctaaaat taaagggctg gtttcattgc tatttctcca attccctcta gtaaagctat
    10441 gtgcaaaatg ccaatatcct atattttcat tatattgtat actaactagc tctcctaatt
    10501 ttttgctttc tacaatttct tttaatttat tataaaatgg agaatatctc agtacatggc
    10561 aaatcataaa aatcttatct ttatacttat cacacaagtc atctatatgt accagaccat
    10621 ctaaactatt agacattggc ttttctaata gtacatgata acctttttca agagctagtt
    10681 ttgctacatc ataatgtcta tcatcatttg tagctattat tactgcatct gccatcttgt
    10741 catgtttaaa aaagtcttct aaagtttcaa atacattttt actatctagg ttatattttt
    10801 gtgtaaatag ttctcgtctt ccttttttag gttctgctac agcaactatt tcacatacat
    10861 cagaattttc ataaatatat ggagcatatg catacgttcc tctattacct gctccaatca
    10921 cagcaacttt cactttttcc atactttaat ttatcccctt tatattattt atactactct
    10981 attatagtct atttttacag acatttggat atttttaata aaagaaacag cagttataat
    11041 ttttcaattt taactgctgt tataagttaa tataccaaac atttttaatt aaattttctt
    11101 taattttaat acaaagactt aaatattcaa tacaaattat aaaaacactt ttatctagtt
    11161 tacttcttct tttctttgtc taatttatct aacttcttat tgtataaaaa catataaaat
    11221 gctgccgaaa atactattaa atatccaaca acacttaaca tatctggata gacattgaat
    11281 aagaatatac ttaaaattgc tgaaaagatt atattactat agtcaaatat agagatttcc
    11341 ttagcaggtg catatttata tgctagagtt attccaaatt gacctagact tgcaaatact
    11401 ccccctagta atagatatat aagttgcata agggtcattg gttcatatac tgcaaaagca
    11461 attggcccta cagtaactaa tgaaaaaact gaaaagaaaa atacaattgt atagtaatct
    11521 tctctatctc caagaactct taaacaagta tatgccattg ctgcaaatac agctcctgca
    11581 attcccccaa gatatggaat tatttctaca ctaaatgttg gttttattat gaataaagct
    11641 cctataaatg caattataat agctcctatc tgctttacat ttattttttc ttttagagca
    11701 attgcacaaa atattactac aaggaaggga ctcattttat ttagcatatt agcatctgaa
    11761 agcatcaact tgtcaattgc atagtagtta aatataactc ctagagttcc aaatattgac
    11821 ctatataaaa gtacttttaa attttctttt ttaccagtta gacttccttt atgttttatg
    11881 ataagccaaa aagctattat agctgatacc agatttctaa agaacacttt ttgaaaactt
    11941 ggtaaatctc cagctagctt tacgaatgca gacatcattg caaagccaag cgctgatgct
    12001 attataaaca ttataccttt tattcgattt gatttgattg tgctactcat acgtacctct
    12061 tttctatata aatgtataaa accatattaa ttaaaaactt agcttataca ttaaataata
    12121 attatctaaa tattccaatt tatactataa ttaaaagtgt aaactatgtc aagtaactaa
    12181 taaatctttt caattaataa caataataat tatagctaaa aagttatatg tttttatgga
    12241 ttattatcag aatatataat ataattttat atagtataga ctttttttaa tttattattt
    12301 agatttttaa tagatttttt aactatggag gtatttaaaa tatggaaata aagagatatg
    12361 aaggaactgg aagaatgagt agagctgtag ttcataacaa tacagtttat ttatgtggac
    12421 aaactcatgc tgaaggagga gtaattgaac aaacaactga agttttagct aaaatagaag
    12481 atttattaaa caaatatggt tcagataagc aacatttgct atctgttact atttatttaa
    12541 gagatatgaa agactttgaa gctatgaact ctgtttggga tgcttgggtt gaaaaaggat
    12601 ttgaacctgc tagagcttgt gttgaagcta gacttgctag agagcatttg ttagttgaaa
    12661 tgagcgttgt tgcagctaca aaataatcaa tttagcttat aggaggctat ctcaaaacag
    12721 agagataagc ctctttttca ttgttgaaat gatgaaaata catttgattt tgtataaaag
    12781 aaagtgtctc acgaactaaa aaattcattt tgagacacct cctcactatt taatctatat
    12841 aaatttactt attctctatt ggcaatatca atttagatgg atatttacct ccatgataaa
    12901 tcgtattctt tgcttctata tattcaatac tattatatcc ctctgtcgtg ttgctatttt
    12961 ggaaaataaa gtttttagca ctagacgtta tatctagtct aattttatga ccaactttaa
    13021 acgtgtttga tatttttgaa gttataatcg taaacttata aatctctcct tcttccataa
    13081 actctgactt ataaaaacta tttctaaatc tagcactaag tataccatca gctaatttaa
    13141 ttgaatttcc atctaaatcc acgtccataa tttttataac ccagtcagta tctttggcag
    13201 aacttgatgc aaaaaactct aactttatat cacctgttac agtaaattct tcactaaaag
    13261 catctgatgt ataacacaac atatcacttc ttttttctaa atctttgtag ttatttggaa
    13321 ctccaacttc attctctgac atatcaatta gttgtactga tgggtctttt ggattatata
    13381 tataactatc atacttttct tctaaaccat tatcaaatac taatctacca tttcctaaag
    13441 atgtgttagc atttccatca cttattagat acatactttt atctatctta tttattattg
    13501 gccaattttc ttctgtcttc cacttattag aacctacaga gtaatactct actggagcag
    13561 tcgtatctat gttattttct atacctttta agtatttatc aaaccataat agataattat
    13621 aatccaaatc atatcttaat gaactatttc ctaaagaaat accatttata tctcttaaag
    13681 tatttgaatt atgcatccat ggacctagaa tagcctttct ctttcctttt tcatagtcct
    13741 ttataacatc taaggcctct gtagttccca tactattatc atcataccac ccagatacaa
    13801 ccaatgcagg tacatttatt ttatcttttt gtaaatgcca atccatcaaa tcccaatact
    13861 catctttttc tactctcttt aaccattctt ccagaaattc aattctatat ccaagagctt
    13921 caacaggtat attttctatt ggtcgtatgt ttaaaacatc atcccaatca tctcttacca
    13981 ttttgcttct atcaacttta tttcttgata ccataaatgc ccatgcaagc ataccagata
    14041 caaaagctcc tccttttctt ggtatatcaa taaaagggct tcctgatgtt actatactaa
    14101 ctaaagcttt aagatgctta ttgcctgaag atgcagctgc ccattggaca taaccaagat
    14161 atgatgctcc aagcattcca actattccac ttgaccattc ctgtgatact atccaattta
    14221 tggtactatc tccatcttcc ctctcatgaa tcataggttc ccacttacct tctgattcat
    14281 ttcttcccct aacatcttgt attacaacag catatcctct ttgtacatat ttataatata
    14341 tttctttatc attttctttt ccatatggag ttctcataag aattgtcggt gcttttttag
    14401 tagaatctac aaaatcaggg agatatacat ctgtagaaag ttttatacca tctttcatat
    14461 caatcatata atttccacca aatcttatag aatatatctc ttcattttta tgagtttttt
    14521 cccattcttt taatacagta tactcttcat atccatccat aattaatata gtacaagttt
    14581 ctctggataa acagataaaa cctactattt tattatctac aataattata tctagtggga
    14641 actttttgtt tctttgaacc catatatatt cattaagttt agaatgcgtt tctcctaagt
    14701 tatttataac tgtgtcttta tctgtgttta ctaagctttt agtatataca tcaaaattat
    14761 taaataaaga ctttaaatct atttttgtaa gatttaaata gtatgtattt tcttccttgc
    14821 ttattggttt taaactttct attttatttg taaaaggatt caacttttca attaataaat
    14881 ctccatcaaa gattacattt ccactgtata gtccagatat gtataattta aattttatca
    14941 attaaactcc cccctataat cctagagaca tctttattgc atatgccact gatatacctg
    15001 cataatttcc taatactcca ccaattactc ccattacgac tgctacactt acaagcgaat
    15061 tccactttgc agaagcagct actaaagctg aagtaggacc atcagcaatt gctcctacta
    15121 tagaaagtat tacatattga tactttatct ttaaaagtct agttatgcct aaatggaaaa
    15181 caaatgaagc aaaaattatg ctaaaacagt aaagtgttat agtaaaagtt gaacctaaaa
    15241 attcttttat atctactgaa aaacctattg tacataaaaa gaataatgca acaaataatc
    15301 ctaaatctaa atttcccttt agtttcttta ctggctttaa ttgagcaatt ataattgaaa
    15361 ttgttgtaac taaaagtatt cttgctgcac tactaaaact cgaattgaaa tatccagcaa
    15421 gtattgttgc aacttctgta acaacaaaac ctatagccaa catccaagct atatctttaa
    15481 tactccattc ttttgactcc ataaattctt cattttgaca atcctctaat tctgattctt
    15541 ctacatgata tggccaaagt tttttaaact tctttgaatt ttttaatatt gctggtagag
    15601 caaacataaa tatcaatgtt ggtactccaa ttacatagtc tgctgcattt gctgcagcaa
    15661 gagtttgtct acttacatta agacctgttc caatagctgt aagattagca cttccaccag
    15721 tatatgtacc tacaaacatt ccagcaacct tccaaccttc tgatattttt tctgcaaata
    15781 ctaatccaaa taaaaatgcc atgatactta cagttaatac agctacaaaa attgcaatta
    15841 aaggttctct tgataatttt gtcatttctt ttaagtcaac actcatcaac attatagtca
    15901 tagaaactgg aatagcatat gttgaaatag ttccatataa ctcagtactt actggaacta
    15961 cttttaagtt tgaaagtatt attcccatta tgattattgt aagtgctggt cctaagcttt
    16021 taatggcttt gtatctttgt aagtaaaaac ccaatgcaat cattgatgtc attacagcaa
    16081 gtatactacc tggatttgaa aaaagtgtat tcatatttaa cctccaaatt tatcataagt
    16141 ttacttctat tcctaaacct ggtttattga caagattcat aatatctcca tccattacaa
    16201 atcctccaga tataccttct aattcttcac aaaacataaa actatcaagg tcagcttctg
    16261 ttatgttctt tttagatgct attaaatttg ctgctgctgt tataccaatt ctcgtttcca
    16321 gcatacatcc taacatacaa tttactccac ttgcctcagc tatgttgttg atttttatag
    16381 caggatatat tccacttgat ttcatcagct taatattaaa tgtatcaact gcattctttt
    16441 ttatagcctt aattgcatct attggtgaat gtaatgattc atctatcata atttttgtat
    16501 tacttttatt tcttatgtaa gcagtaccat ctaaatccca atgtggaagt gcttgttcaa
    16561 ttgcatcaac attaaattct tctaactttt tgatagtatt tatactgcta tttacgttcc
    16621 atccttgatt tgcatcaatt ctaattctta tactctctcc aacggcttct ctaattaact
    16681 ttacagcttc aatatcgtct tcaggattta tacctgcttt aagcttcaat atcctaaaac
    16741 cttcttttac tctttctaaa gcttctactg ccattttttc tggcttatct atacttatag
    16801 taatatccgt ttggacttta ctatcaaatc ctccaagaac tttataaagg ggtgcattca
    16861 taatttttcc tttaatatca tataaagcta tgtcaatagc tgcttttgca gaagtattac
    16921 ctataataat tccatccatg attgtatgta tggtttcaat atctagtggg tctttgccta
    16981 ttaactcttt tttaaacata agcaatactg aaagaacatt atccaaaact tcacctgtaa
    17041 ctgctgcaag aggcgctgct tcgccaaatc cacaataccc ttcatcagtt tcaattttta
    17101 aaattatact ttctccatat tcaattactc caaatgaaac tactacaggt ttttttaatt
    17161 taattctaag cttttcaaat ttgatatctg ttatcttcat attaccctcc ttattttttg
    17221 aagttataat tcaattttta aaattttata ctttaaatta tattattact tcaatatata
    17281 tgtcaagata atttactgaa aatttactct tttccaaaat ataatgtgat aatcttaatt
    17341 caattaatac tcttctatta aattttttta ttattatttt ctttgtatta atttaacaat
    17401 aattcatttt catagacttt tatttggatt taattaaatt ttaattgtca aaatctacta
    17461 tgtattctta ttgccactac ttctatgttg tgctataatt actttgtttt aagcgaatat
    17521 tttatattaa ttttattatt tggaggttct tatgctaggt gaaaagatga gaaatataag
    17581 aaaaagcaag aaaaaaacat taagtgatgt ttccaaattg acagatttat ctataagtta
    17641 tatctctcaa atagaaagag atgctattga accatccttg tcttcattga gaaaaatcgc
    17701 tgaagtttta gacactcctt tatatatgtt tatggatgac aataatactg atgatttagt
    17761 cattagaaaa gaagatagag ttatgatgaa atttccaaag agtgaaatgt tttacgagat
    17821 agtatctcca atgcctacaa ctgagtttac accttcaatt ttatttgtag agtttgaact
    17881 taaaccagaa agtgaggatg ccaaagatta tatatatcat gcatcagaag aaattatggt
    17941 ggtaacgagt ggtgtggttg atatttgtat gggtgaaaag gtagttagat taaatccagg
    18001 tgactcaaca tttataaaag ctaatgtacc acataaaatc atcaatccaa gtaaagatac
    18061 aatagctcgt ggatatggag ttatatcacc tccaatttgg ccaataaaat ctaaataaac
    18121 tcaatatgac acacctactt tttgtagata atataataga tttaaatatt gcttttattt
    18181 taaaattgaa gtatttattt aaatttaaaa tatttactca aactaaaaaa atagctattt
    18241 gactaatcta aaccatctta attatggtaa atagtaaata gctatttgta agttatatta
    18301 taaactataa taaaaattgt atttgttaaa attaaactgc tatatcattt aatacttttg
    18361 cctttacaac tcttatattt ttcatactct tgtagatatc ttgaatctta tcttcttgct
    18421 ctctcaatag tctatgtgct tcatatatat ctacaagttc aaattttact acatcatttg
    18481 gctttgcttg tgctaactta tacaaatcta cacttataac gttccctatc ttagtatagc
    18541 caccactagt ctgtctatct gataacatta taataggttt tccatgacca ggtacttgta
    18601 tagctccata agaaataccc tcagatataa tatctgctcc attaaggtgc tttatactct
    18661 ctccatctag tctacaaccc attctatcta tttcattcgt aactttgtat ttatttgata
    18721 aaaatttctc tacttcaccg ttagaaaaag catcaaattg aggacctttt acaattctta
    18781 gtacgatttc tgaactataa gttggaatat attttgaact taatttttta tttattgtaa
    18841 aatctttttc ctgaataaaa gtatttatat aatcgccttt tttaagtgct ctaccttcaa
    18901 atccaccaat tttagctctt gtataagttg acttacttcc cattgcaagt ggaacatcta
    18961 ttccaccagc tattgacaca taggctctag tacctgattt cacacctcta aatgataata
    19021 catcacctgg atttatttga tatgacctcc agttttgaat ttcttttcca tttattttag
    19081 cccctaaatc tcccccagtt actgctattt gcgtatattc atcaaaggta attgtaggac
    19141 ctaacattaa aacttctaaa gaagcctcat cttcagaatt tcctactaat atatttccta
    19201 ctctatgtgc atactcatcc attgctccac atccagatac accatattga ccatagccaa
    19261 ctctacctct gtcttgtatt aatgtttgga aacctcctaa ttcaactttg aatcccatat
    19321 ataccactcc ctgtactata acgatattta aatacttaag ttattttttt cgatttctat
    19381 aaattcatct aatgttattg gaacaaattt tataaagtct ccagctctaa gcaaaatctg
    19441 ttcttctcta ttttcatcat ataattttaa aggtgtccta ccaattattt gccatccacc
    19501 aggactatca ataggatata caccagtctg ggaacctgct attccaacac tcccaccata
    19561 aattttagtt cttggcactt ctaatctagg tgtagctatt ctttcatcca taccacctag
    19621 atatggaaat ccaggagtaa accctagcat atatatcaag tactctctgc ttgtgtgtat
    19681 ttttataact tcatctacag tgagattatt gtagcttgca acgtttttaa tatctatacc
    19741 aaaaacctca tcataacata ctggaatctc atgtatttta ggttttggta atactatact
    19801 ttccaaatta tcttctattt tttttaaatt ctcaatcaaa ctatcaaaat ctatttctaa
    19861 tggattataa cttatcataa gtgacctata tgttggtacc atttctgtta caataccttt
    19921 aagattcgat atttctattg ctctcataaa tgaaattact tttttgttta tatcttctga
    19981 tatctcattt ccaaattcag caaccactgc cttatcccct gatatcaagt atctagtttc
    20041 cataaatgtc acctcttcta aattaagcaa ataaagcacc taggctgcta agagaagtta
    20101 ttcctgtata tgcagatatt aaaactacta ttaatccaaa tattaaaagc catgtaggat
    20161 gcttgtaatc tccaacaata tcttttcttt tagatgctat aagcataatt cctaatgtga
    20221 ttggaagtat caatccattt aaagctcctg ctaaaattaa taaagtagct ggttttccta
    20281 taaatgccat tataagtgtt gatattgcta taaatgctat tataaaatat ttttcatatt
    20341 tatctataaa aggatttagt gtctttaaga aagaaactga tgtataagca gctcctataa
    20401 ctgatgtaat tgcagcagac cataatacaa gtccaaagaa tttatatcct attgctcctg
    20461 cactaaattt aaaagctgaa gctgctggat tttctgggtc aagttgaaga ccttttgaaa
    20521 ccactgccag tattgctaaa aataaaagaa ctctcatcat tgtggcaact aatataccca
    20581 aaagagaaga tttagttatt tcttttatat tttcttctcc tgttattcca ccgtctatta
    20641 atctatgtcc tccagcaaaa gttatataac ctccaacact tcctccaagt aaagttatta
    20701 ttgggaatat cagtcctttt ggattttctg gttttaccat tcttgatact gcctctccaa
    20761 caggaggatg atttgatatt gctacatatc caactataat tatcattcct aatgctaaaa
    20821 atttagttat cttatcgact agagaatttg aattttttga cataaataca aatatagcta
    20881 ttaatccact taatacagtt cctaatgtca tattcatatt aaacattaca ttcataccaa
    20941 gtgcagctcc accaacattt cctatattaa aagctaatcc tccaagagct actaaaaaag
    21001 ctactaaata gcctaatcca ggtattattt tatttgctat atcctgacct ctaagtcctg
    21061 atacaccaat tactctccat acatttactt gagcccctat aaaaaggatt gttgtaatta
    21121 aaattacaaa tgaaaaattc ggtccaaaat cttgggtaaa ttgtgcagtc tgagttaaaa
    21181 accctggtcc aattgctgat gttgccatta taaatgcagc ccctataagt gctccgacat
    21241 cttttttatt tttagtttta tctgtaatat tttttgtact catattttat ctccccctca
    21301 tgttatcagt cttatactat gttctctgac gaacaaactt ctatgttttc taatggacaa
    21361 acttctatac cttctaattc aaaacgcttt cttatttctt tgacaaactc tattgctttt
    21421 ggattgtccc catgtacaca tatagaatct gctttgatat gtataacttc acctgttaaa
    21481 gtttctacag ttccttcttt taccatccta acaaccctat ctattgcatg ttttgtatca
    21541 tgtataactg ctccttctac atttcttgga accaaaaaac cattattatc ataagctcta
    21601 tctgcaaata cttcatttgc aaacctaagt ccaatttctt tagcagaatt tatcatttca
    21661 ctattatata gccccaacag tataatatct ttatcgactt catatatagc ttcacaaatc
    21721 cccatagcta actctttatc tttagctgcc atattgtata atgctccatg tggttttaca
    21781 tgttgtatat tacatccatt tgaacttgca aatgccatta aagcacccag ttgatatttt
    21841 gtataatctt tagcttcctg ctttgttatt ttcatctcac gtcttccaaa tcctattaaa
    21901 tctaggaaac ctggatgtgc gcctattttc actccatttt ttttagctag ctgtactgtt
    21961 ttttccatat gactagggtc acctgcatga aacccacaag ctatattggc tgatgaaata
    22021 tattttaaaa cttcttcatc taaaccaatc ttatacgttc caaaactttc tcctaaatca
    22081 ctatttaaat ctactttata caatttatga cccctcctcg ctaacatttt gatagaataa
    22141 gtagttttaa tttttaaatt ttgtttattt tatatattgc atgaaatatt ttatcacttg
    22201 tattcaattt tatttttgca actattctat tgcatttaat taatataatt ttaccattta
    22261 aggaatattt ttgcaattat tttaattgaa cattcatttg tatttctttt tcaaaaaatc
    22321 tttttaccat tttttagtta ataattaatt attttcactt aaaataaggg ctttaacctt
    22381 catttttaaa gtatttttca tttttaatca ctttatatgc aagacagaat catttttctt
    22441 cattcatttg tttctttcat ctatataatt atcattcttt gtaagtaaaa tatctttata
    22501 tttttataat taattcttta tatagtttta agtataataa aatcatgtat actaaatgca
    22561 tgttaaaata ttattttaaa acatgcacaa tatttgtaca tattgtagac atataaacct
    22621 ataaattata caatagtatt gttaagatta tatatttttt taacataaat tttgtattgg
    22681 gggaaaaaca atggataaaa actatacgat gccaatatac caaaaaatag cgttggatat
    22741 tgcaaataaa atatacacgg gtgaaataca agaggattca gtactatttg gacgctctgt
    22801 tttggctggt aaatacaatg tatctccaga aacgataaga agagctgtca aaattttaga
    22861 ggacattggc gttgttaaaa gcataaaggg taaaggtgtt attgtactat ctccagacaa
    22921 agcttcttct tttataaaaa aatatagaga tattacaaat atatcatcat ataaatcaac
    22981 actttataat ttaatagata ctaaatctaa tttagaaaat gaaatattag atactataaa
    23041 taaaatttta gactattcta acagacttga aataatcaat cctttagtgc ccgttcagtt
    23101 caccataaat tctaattgca aatatattgg tcaaacagct gcacaaacta aattttggca
    23161 aaatacaggt gctacaatag ttgcaattaa aagaggtgaa gaacttataa tatctcctgg
    23221 accatatatt gaatttttag aaggagacat tctcttagta gttggtgacc aacacattta
    23281 caattctata ccaatgtttt tatatgaaaa cgaaaaataa attatataat ttagtgtgat
    23341 tatataaaaa tgcaagatta acaacaaaaa aagagcttca tattttacat aaagctcttt
    23401 ttaaattcaa ataaatttaa ctttgattaa tttttaaata tccaattcat cttctttttc
    23461 ccagcttaaa gctctcgata cagctttttt ccatccttta tataatttac atctcttctc
    23521 ttcactcata tttggattaa actctttttc tactgaccac tttgaagtaa tttcatttct
    23581 tccaatatag aatccaacag acaaccctgc taaataagca gctcctaaag ctgtagtttc
    23641 tactattttg ggtctatcta tatctacatt taatatatct gattgaaatt gcattaaaaa
    23701 attattatta cttgctccac catcaacttt taatgatttt agttttaatt ttgaatcgtt
    23761 ttgcattgct tctaaaacat cctttgtctg ataagcaatt gattccaatg tggctcttac
    23821 tatatgttct ttttttgctc ctcttgtaag tcctaaaatt gaacccctag catacatatc
    23881 ccaatatgga gctccaagac ctgtaaatgc tggcactaca tatacaccat tagtatcctc
    23941 taccctattt gcatacattt cactatcttt tgcagattca attatcttta attcatctct
    24001 aagccattga acagatgctc ctccaataaa tatacttcct tctaatgcat atgttacttt
    24061 tccatcaact ccccaagcaa ttgttgtaag taacccatgc ttagatttaa ctatgttact
    24121 gcctgtattc ataagcaaga aacaacccgt tccataagta ttttttgcag taccttcttc
    24181 aacacaattt tgaccaaata atgctgcttg ttggtctcca gcacatcccg caataggtat
    24241 tttagctcct gataacatgc gctcatctgt atgaccataa acatagctag aaggttttac
    24301 taatggcaac atgcttattg gtatgtccaa ttctcgtaat atatcctcat cccattctaa
    24361 atcatttata ttgtacatca ttgttctaga tgcatttgag taatctgtta catgtacttt
    24421 tccacgagtt aaattccata ttaaccatgt atctatagtt ccaaataata actctccttg
    24481 ttctgctttt tccctagctc cttctacatt atctaatatc cattttactt ttgttgctga
    24541 aaagtatgca tctattaaaa gacctgtttt ttcttttatt gattcttcca atcctttatt
    24601 tttaagttca tcacatattt ctgacgttct cctacactgc catactattg cgttataaat
    24661 aggtttacca gtatacctac tccaaacaac cgtagtctct ctttgattag ttatccctat
    24721 tgaagcaatt tcttctggtc ttatacctgc tgtttctata acttctctca ttaccccact
    24781 ttgtgacccc catatttcca tagggtcatg ctctacccat ccagcttttg gataaatttg
    24841 attgaactct ttttgtgcaa ttttcactat ttcgcctttt ttattaaata gtatagctct
    24901 tgaactggtt attccttggt ctaatgccat tacatacttc ttttccatca ccatattctc
    24961 cttatcatat aatatttaat ttattaaaaa gaaaaattta atatcacata tttcaataaa
    25021 taatcatact taagtacaaa tttaatataa tagatattta gtaagaagac acttattttc
    25081 tatcatatct atataataat attatacatt atagcttaca tattgattaa taaattttaa
    25141 aggagtataa taaacagtgt tcataataat tttaataaaa gaggtggtaa tatgccaaag
    25201 attttagaaa atgtaaaaga agatatatta aaagtgtctc gggatatgat tttagaagaa
    25261 gattactcga atattagcat aaggaaaata gctcaaaggt gtggtataag tgcaggtaca
    25321 gtgtataatt actttaattc aaaacaagaa ataatagaat atatcacaaa atctgaatgg
    25381 gatttattga taaggcgaat tgaatactcc aataaaaaca ctgaagatta cataaaaaaa
    25441 ctaaacatta tttttactga aataagaaat tttataaaca aagttcataa tatccaatac
    25501 aatgactttt taaacacttt tgaaacagag agattttttg aaatgaaaaa acacaaagac
    25561 gactttcatg aacagctttc aaataaagta tatgaagctt tagaaaaaga aagttttttt
    25621 aataatgaca aattagtatg caatatcata ataaaaatgt ttttttcata ttcaacatat
    25681 cttcatatag aatttgaaga tttaaaaccc tatattgaaa aattaataca ttaaggtggt
    25741 gtttaccttg aatcataata ctagtattaa gaacaaggac agtaatccaa aaatattttt
    25801 agtacttacg ctttatctat taggtatatt tatgggagct attgatactg gaatagtatc
    25861 accagcaaga acgattatac aaaatagcct tggagtaaat gaaaaaacgg gaatttggat
    25921 gattaccata tacacattaa catacgcttc tgtaattcct atctctggaa aattagcaga
    25981 taaaatagga agaaaatatg tgtatctagt tagtatattt ctatttggta gtggttcatt
    26041 aatatgtgga ttatcatcac tattttccaa tttctatatt ttattaattg gtcgtgttat
    26101 tcaagcaatt ggtggtggtg gaatcatgcc aattgctaca gcagaatttg gaactacctt
    26161 cccagaaaat aaaagaggca tggctttagg tttagttggg gcaacatatg gtattgccaa
    26221 tattttaggt tcaagcattg gtagcacaat tctaagtatc tttggaactc aaaattggaa
    26281 gtggctattt tttgtaaatc ttccaatttg tttaatcata cttattggtg gagttttctg
    26341 tattaaaaac aataagtctg aatctaaaga aaaaatagat aagcttggta cattgatgtt
    26401 gatatgtata atcgtatcac tattatatgg tcttatgaat atagactttt ttaattttaa
    26461 aaattctata caagatatat cagtttatcc atacttattg acatttattg tacttatacc
    26521 tattttcata tttattgaaa atagagctaa agaccctatt ttaagctttg aatatttttt
    26581 aaatccgaga atattgatag tacttatttt atctttaatt gttggtattg gaatgatggg
    26641 tatggtattc gttccacagt atgctgaaaa tgcattaaag ataaatgctg gttctggggg
    26701 atattttgta gctatattgg gattatttgc aggtattgct gctcctttaa gcggaagact
    26761 tattgacaag tttggggcaa aaaaaatact attactagga ttttctgtat ctatgatagg
    26821 ctctctttac ctagttttaa ttgctctaaa aaccaatact gtatttagtg tatgtgtaag
    26881 tttaatattt atgggattag gaatgggatt tactatggga actcctctaa actatatgat
    26941 gttatcaaat acaagattag aagaatctaa ttcagcactt gcaacattat ccttgatacg
    27001 ctcaattggt acttcaattt ctccagctat aatgattgga tttatagcac atgcaggatt
    27061 atctgtgcaa gataatatta tggacttagt tggaaagccc tcaactccta aaatagttca
    27121 acttgaagaa cttgaaaaca tgatagatga cttaaaatca gaccctgaaa tggcaaaaaa
    27181 tttaaaaaat gtctctatcc caaatatgaa tgatacttca aatataaata tggacatgac
    27241 aggtggcaaa cttccaaaat ccttacttaa taaagttcag tcagcagata caaccaacat
    27301 taccaatata acaaaagaaa tatcaacaac aatgtttgat gaaaaagttc catctataat
    27361 tactaaaata cagaaaaatg tacaaaaagg aatagatgga acacaaagtg gaataaacgg
    27421 tattgaagat ggtgaaaata aactaaatag tggtattaaa ggagttcaaa ctggtattga
    27481 caatatggta aaagctagag ctggtattaa tcaaggaata gatggtgtaa aaaaaggtat
    27541 tgctggtgtt gataaaggta taaaaggtat ggaacaaggt cttgaaaaac aagatgaggc
    27601 catcaaggaa cttacaactg cttataataa aatacctact acagaaaaaa ctcctactga
    27661 taacgaaaat aactcaaatg aacaaaacca gagtaatacc agtaattctg aatcagacga
    27721 cactaagaat gattctacag aaaataagaa taacaattcc agcgagaata aaccaaatgt
    27781 aaatgaacaa aaagaaagtt taaatgctca aatacagcaa ctaaaaaaga caagagatga
    27841 cttaaataca aagttacaaa aaaataaaac tcaaaagaac gaattaagta aaaaactaag
    27901 aacaatggaa tctcaaaaga aagagctaca aagcaagtta aataattcta taagccaaaa
    27961 gaaagacatg gaaaaaagcc ttgatacaat gcaacagcaa aaaaaatcac tccaagcagt
    28021 tttagcaaag acacaagagg taaaaactga aataccaaag gcttttgata aatcaaaatt
    28081 ggattatata aattccatag aaaataatag aacaaaaata gaaaacacac tacaatctac
    28141 tctaaactca ggatttaaac agatgtatat aacagtattc tgtgtaaatc tactagcatt
    28201 tataatatta ttgttctata aagaaaataa aactagataa aagagtgtta tagtattata
    28261 ttgtaaattt atcttattct taaatatttt attctaaaga ataaattcat aaaatttttg
    28321 taattgatta atataaatta caatataata ctatttttct cttaatcttt ttctctttta
    28381 cagacaatca aaaagcaatc ttagtttatc gaaataaatt tatacaatct attcatacac
    28441 ccctttacat ttatataaca attaaaccta ttgttatata tacaaatcaa aataaaccat
    28501 atattttcaa ctataatatg ttaaaatcat attatgagca tattaattta agcaaaatgt
    28561 catttagact ttaatttagt agttttatat tataaattta tatattttta attttaaatg
    28621 tcatttagac attactcata aaaagaaagg agtgtatttt gtggatactt taggaaaaag
    28681 aattgcttat ttgagaaact ccaaaaaact tactcagcgc aaacttatgg atattttaaa
    28741 atttgaaaac ttaggcaagt atgaaacagg agatagaaaa cctaattgtg atatactaat
    28801 gtcaatagct gattatttta atgttacgac tgactggctt ctctatggta aagagaaggt
    28861 aaatgtcaat agtagtgtaa aagaagataa agaagattac ttacatgtta ctaatgatga
    28921 aatgacgata ttaaatctat acagacaact aaacgaacgt gataaaataa aaatagaagg
    28981 aatacttgaa cttaagatat ctgaatataa agacttaaaa aagcattcct ctaataataa
    29041 tgaagataaa atggtttaaa catacataat ttgatacgat tttacatcat ctattaacct
    29101 tctttttttg tagtatatta ctaaaatatg gatattggat tatttaaaaa caagtatagt
    29161 ctaatttcaa gcacatttat aatatttaga ttatcttatc catattttag tttaagtact
    29221 ttgaaaaata taatacatct ctatgtataa cttatattcc taagaaatgc attactcatg
    29281 tttttaattc ttccaacatc tttctataca caattgtaca tccttcataa ttcccctctt
    29341 taagaaggat tgaacatgta ctagaatatt tagttaaagt atttaatctt tccatattta
    29401 agctttttat attactgagt gctgtaataa aatcatcaat tgctctacaa cactctataa
    29461 ccccatctga atccaattta cacacaagaa tattcaccta ctttatatac tatttattaa
    29521 tcatcttctg aatattctat attaagtaat tgataaattg atgatttata ttttttacct
    29581 gttcttcttc catttaaaat agctgacaaa tagttttcat taacacctat tttcttcgct
    29641 aattcccttt gagtcatatt taattcaata agtctttgct tgacttgcac tcccaaaaaa
    29701 ggtatccatt tttttcgagc atccatttat caacttcctt tctaagtgat ttatatttac
    29761 ttttatatta ttttagctta cacaccccct ctatttagta tatattttta atatgtacac
    29821 atattaagta tataaattta tttaatacta aataagattt tttgattact ttgtgctaaa
    29881 attaggatgt aaattcgtat cattaagtta attataaagt cattttgacc ttaagtcaat
    29941 gctttttatg tcattttgac cttaaatttg aaactattta catcttaaaa agtctttttg
    30001 acattaattg aatggagagt gtttatggat actttaggag aaagaattgt ttatttgaga
    30061 aaagcaaaaa accttaaaca atatgaacta gaagaaatgt tgggctgtga caacttaagt
    30121 aaatttgaga gaaacattag aaaaccaaat tatgaaatat taaaatctat agctgagata
    30181 tttaatgttt cagtagactg gctattaaat ggagataatc tatcacataa atcagattta
    30241 atttgcgatt cctcctcaaa ctatccactt aatagcataa actctaatga aataaagctt
    30301 ttaaacaatt ttagaaagtt aaatgattat gataaagcca aaatagaagg tatgattgaa
    30361 ttaaaacttc atgaatatga aaaagagaag gatttaggaa aaattgagta taacaaaaat
    30421 aaagatgaga aaatagataa ataattttta tgttgctttt gatacgattt tacatcattt
    30481 ctttgttcta atatttttat ttcatttatt ttctttttta taattttatg ataaaataat
    30541 tagtatattt tgctttactt gaatgtaatt atttatacaa aagtcaaaca taatataaat
    30601 gtagtaaatt tttagtttca agtaatatct cgttattata actaaatatt tattaaaatt
    30661 aggaggataa aatgggctta agagacaaat tcgcgcaatc atttgctaga tcaaaaacta
    30721 tgagtggccc tgagaaaaag gctaatgaaa taatgggtaa attgcttctt aagaaagcta
    30781 tattacctat cgttttaatg ttcgtgataa taatcgctgg agctatgctt aaaatcaatt
    30841 cttgggttac acttggcata aatttagtta ttgctgttgg tgctttcttc tacataagaa
    30901 attctagtaa aaagtatcag aactttaaac cttatgttgg caatttgatt agtttagaga
    30961 aaaaaggtaa aaaagagtac gttgctatta taaaacaagg taagttacca gttaagttac
    31021 aaatagctta tggtggtgaa gaccttgaac atgttaagaa aaatcaaatg gttcaaataa
    31081 gttataatcc agatgctaaa atagctattt tggttaatag acaataattt aataaaaaaa
    31141 aggagttgtc ttaaaattga ttttttctaa tcaaatttaa gctcaactcc ttttttaatt
    31201 tttatatatt atttttatat attattttta ttactttttt taattaatat ttttaaatat
    31261 caaatccttc tcaataaaat agggtactat ctacaatact actttatctt ttcctaacaa
    31321 tatataatct ggtgctactc tttcatgtta tacaccgttt tgtaacatac cttttatacc
    31381 agtaacatct tccttaattt cctctacatc tgttttcatt atacccattt gaacaagtat
    31441 atttttgttt atttcttgtt gttgtgtaga taattctata aaattttcta cagttttttt
    31501 atacatgtct ctatcttctt ttttctcctg catagtattt ttaaataata gagcacatag
    31561 tatacctatt gcccctagac tactcaattc ggttattaat ttatccatta tagcctccta
    31621 ttgtctaaat tttttatact tatagcttta atttttgtta tattcttaca tcatgcaata
    31681 ttatattttc aggattatca atttaatctt ctgtacttta aattgtaggt gcaatttata
    31741 gattgaataa ataataaata tatatacaat ttctaatata acaaaaaata agtacaaggc
    31801 ttacttatta aagttaagac cttgtacttt acataaaaaa aataactaat acatagttat
    31861 tctaaattta ctattcaact ggtatttcta attctttgca tctaattcta acttgctctc
    31921 taattactgc tggtacttct tctattgttt ttttgcctct taaaattaaa gctacatata
    31981 cttctaccat tccactctca cctccagatt ttcttacact aaaagagctt atattacttg
    32041 cttcattgac agaactttga tttaacaaca tctcatatat ttctgcattt gctataaaat
    32101 tatttgttat atcatcatct tgctttactt gacctatact tatgtctgaa atgtctttat
    32161 actgcatttc tacttttcta ccttgtttga tttgttctat tttattttta tcgaatttaa
    32221 acaattttca cacctccatt ttaagcttgt ggctcgttgc tttcagcatc atatctaaaa
    32281 atcatagatg gacgttttat agctgtccat ttatctcctt tttctactat gattttaggc
    32341 tgagcacttt ttatgttggc ttcttctaat tcatagattt cttggtaact tgagtctgat
    32401 tttttgattt caactagttt catgtctttg aataagtcag atttatttat ttttacttga
    32461 actttagttt tatttattaa ttgatatata ggattgtttt ccatcatgtc atcctttaat
    32521 ccctcattta atgttactat taaatcagtg ttattttcag taatgtttat gattgaatat
    32581 actttattgt ttatcattaa aatagaatcc ttttttactt taagccctgt ttctgattta
    32641 aatataaaca tacttgaata taaatcgcct gtaggactac tggctttaat cagtatctta
    32701 ttttctccta atactatttt attttgtggt atctcaaata taatgctatt tatttccaaa
    32761 ttagtcttag tagatattaa ttcatcattt ataaacattt caagtttttc tataacatta
    32821 ttagggtctt gtactacaac tctcacttga gatattgatt tttcaactat ttctatagat
    32881 gctttacttg tatctacttt tgttatcatg tatattgtat catctgaaag ctctatatct
    32941 aaaatatcat ttagatgttc aggaattggt attttagtaa attcatataa atcagtaatt
    33001 gatatagata ttttatacat agatttattt ttaccagtta tccatagact attatcattt
    33061 ttaattacta gtatgtggtc atcattatgt gacttaattt ctttaacatt agttaatact
    33121 ttagtaaaag ttcttattat cttatctttt tcactttctc ctagtccaaa taatccattt
    33181 gtattgtacc cttgagcgta caactctccc tcattagtta aaacaagtgt cattgagtta
    33241 tatatactta tatctttaac agaaggtaat ccagtaacct tttgtaactc tcttacatca
    33301 ttattgtggc caactcccaa ttggtactca gtattctttc catatgacca tagagtatta
    33361 tctgtcttaa taatatagga gtagttatct ccacaagcaa cttttttgac atctgatata
    33421 tttacttcag taagagttaa taattctata tcttcaaggg tttgattaat ttctttatat
    33481 gaattacttc ctattccata taacttgcca tccttagcaa cagccaatga atgtgttttt
    33541 gatatatcaa tatcagatac ctcaaatggc aatattattg aagtatactt aaaattatct
    33601 ttaaatatgt ttttataatt tatatactta ttatttgttt ttatatactt atcattaaac
    33661 attactagct tttcattttt cttattaaaa actattttat ctgatgtgtt atctgtttta
    33721 aaatatgaat gtagggaacc ttcaagagct tcatttttat cattatcata tctatggtga
    33781 atacctaaat gattatatct attatctcct gttgaagaat aaaattttcc atatatatct
    33841 ttaataaata tagtattgta actagaaaat acagtatcaa catctttctt tatagatact
    33901 ttataatatg gaactgacat atccctataa gattcatcag atataccata agtatctcta
    33961 cctgatgata tcaaattgtt attattatct atataaagtg tattattagc tgttacaaaa
    34021 acatctttaa catcttccac actatttgta ttattattat ttgctatact tccatatcca
    34081 ctaagacctc caacaaatag tttattgtca tttgttaaaa tggctgaagt atatccatta
    34141 actactattt tttttgcatt tataatatta ttaattactc gtataccctt tatatcatta
    34201 gtttctgttg aaaagaatac cctctctttt ttccctgtag tataaataac attgtcattt
    34261 gttataaaaa atgtagcttc acttcctgct gttacaacag attttacatt ctcaatattt
    34321 atttttgtaa atgtacttat acttccttca gtaaacccaa aatatccatt tacaaatcca
    34381 catacataac atgtattatc atttttaaca attattgata catcattaca tatatacaca
    34441 tctctaactc catttatagg tatacgtgta aatatattcc tacttgtagt atcacccaat
    34501 cctaactgtc cttttccatt attacctgct ccatataaaa gaccatcatt agttaataat
    34561 agagatgtgt ttccatatac agctacatat tttacatttt caacatttat tttagtaaat
    34621 acatttctat ttacattatc tcctattcct aactgaccag aattatttaa accaacagca
    34681 taacaggtat tatcattttt tatcgcaaat acatgtttac tattgtcagt aaatctattt
    34741 acatttatag attttatatt atcaatattt attttagtaa atacctttct atttatctca
    34801 tctcccactc ctaattgacc tacatcattt aaaccaacac tgtaaagcat attattatta
    34861 gttaataaat atgtagactt cgaatggcta tatatttctt gtatattaga agatatttca
    34921 ctactgctat tctttttatt gtaatttatc ttattattta aatttaaact actattaact
    34981 gatataccat gcgtaccctt agagttatat ccacatataa ataagctctt atcagttaat
    35041 attgcaaatc tagttttatc tctacttaaa tcataaggag gtataaatat attttttata
    35101 tttttcatta cctttttata attagtggca ttatatccat cttgtgtatc tgaaacatct
    35161 agttcttttg cactttcaga tatattccca aaaccatata acgtgttaga agataaatac
    35221 aatagagctt tatataaaaa tatggcttta tcagaggtaa taggacctga atttatatta
    35281 gatgctgcaa tattattaga tgattgattg ttaagataat tccttccata ttgatgtata
    35341 taagatgaac ctccagaaat aagtaaatta tctggattta cattttttaa attacttgtt
    35401 gattctccat aatacaaata ggcaaaattt gtagaatatg atgattgtgt acaccttgtg
    35461 ggataatagt tatatatgta attactaata tatccagcat gtgtaattcc ttgttgttga
    35521 actctataag tagaatcact tggctttgtc cataaagtac catggtatcc agttaaaaaa
    35581 gaaccataat ttgtacataa tatagccata tattcatata taataatatt attaatttta
    35641 gtaatatcat ttgtacaaga atttgtacta taacttgctc ctgaaggatt tacataaaac
    35701 cagttattta ttaatgttaa atttatattg actccatcat taggattaac acccaataaa
    35761 tagtttgtat ttataccagt tgcatatacc tttttttcgt cagccacaat aaatgttgca
    35821 ttatatgaac tatatgcatc tgttacattg ctaacaccaa tctcagtata ttgacttgct
    35881 gatttattac taagtccatt aacataactt gaactctttc cagaagtata tatttgctta
    35941 ttattagtta caattaatga aaaccagttt gataaatcag tatttataac aacttttttt
    36001 acattatcta tagatatttt cttaaatata tctgaattag aaggaagaac acctgctatt
    36061 gagttactat ttggcccaga ataataacaa gtgtcatcat ttttaatgta gaatattcct
    36121 ccagaaatat tttttgatgc tactatagac tttacattag agatacttaa ttttgtcaac
    36181 tcatttaatt gacttggtaa gtcagaaccc attcctttgt atctattatt tcctagacca
    36241 tatacatctc cattccctaa aataaccaga gtacaataag gactaaaaac gacatctctt
    36301 acagaatgta tatctagtgg aagttcaata tcctttattt ccatttcttc ttttaataaa
    36361 gttaatttat cctcagattt aattaaattt atataattac tataactata tttttcacta
    36421 tccattttct taaattctac aatgtgcata tcatcaaaaa acgaacctaa ttgtccataa
    36481 gtattatctc ctgtacaata tgaatcatta tttgaatcaa ttagtatgct atgtgataaa
    36541 ccaatgaaaa tattcttagc tacaatattt tttaaatcta taaatgaatt atctatattc
    36601 tcagagtaat ttgctatacc taactgatat atatcattat ttccagcaac cttcaaaata
    36661 cctttattat ctataagcat agtattacca ttaccacatg caatagtttt tatatttact
    36721 tcttctatct tagtaaatag tgatgattga agtgcatttc caaaacattt ttcctttcca
    36781 caaccatata tatcattatt atacgtcatt aacacagtgt gttcagaacc acaagctata
    36841 gtcttaacat cattaataga aactaagcta aataaatctc tattattctt atctcctaaa
    36901 cctagttgac cttgattatt aaaaccagta gaataacatt tattgtcatt ctttattata
    36961 aaagctgatt tattatatga gaatacagcc tttacattat caacatctac ttttataaat
    37021 gtatttacat ttgcagatgc tccattagtt cctagaccta attgaccata caaattattt
    37081 ccagcaacat ataaagttcc atcattgtat aaaagatatg ttgattgaag tccacatgca
    37141 atatccttta cgccttttat ttctggaacc ttctcaaatg tggaaactgt ttttcctact
    37201 ttttctaatc ccaatcccat ttgaccatct gtatttatac ctgcgacaag taattctcca
    37261 tctgaagtaa gtataactgc atgagattct ccacatgcaa cttttttaat attagtagat
    37321 atatttggta ttttcgtaaa taattttctt gttgtattgt cttctcccaa tccaagttga
    37381 aagttactat tatctccaca tccatacaag atattattat tttttagacc aaatgtatag
    37441 ttacaacatc cagtaaaaaa attaataaag tcataattat catacccttc atcaaattca
    37501 tcaactacca tatttttatc gccaaagaaa ttacctcttt gaagtagttt agttcttttc
    37561 attataaaaa cctcctaatt atcttatttc atacgaatat ttatttaaag ttatatctgt
    37621 ctggccaatt gaatatgccc tattagattt tgtttcaaca aagttattat ccatttcatg
    37681 tatatatcta actctatttg cattaaatgg tagaaccaca ttcttaaatg taacactacc
    37741 aagttctcca gttatacact ttctatttaa atctaactta aggccttcta tagctattat
    37801 gtttttagca ttagttaagg tatcatacca atatccagct tcatctacat ctatttctga
    37861 ctctaattct aagtatatta aaatatctaa tactttttca gataatttat tgtaactatt
    37921 ttttaataca gatagttcag tttgtatttg acctcctatt acatcatcaa tagatatatt
    37981 tttaattttt tctactacct catttatagc tcctgataag tttttagctt ctgtagataa
    38041 gagttcttct ccaattttta cttttatttc ttttatattt ttatcaacct tatcatctaa
    38101 atcttgaata tccttttgag ttacaaaaac caacgtcggg tctacttcta atgtaacatt
    38161 tgaagtattg gacaattgta aaatcatttt tacaaccaaa tctttaatac tgccatcttc
    38221 agcacgtggc ttatacgtct ctggatactt acctatagct attatattat tctcattatc
    38281 cagaacagca gcttctctta tcataaatcc accaactgtt cctggtaaaa tagtctctat
    38341 aactatccaa ttaggatttt tttcatcaat tcttagagaa tttatatttc cttcccaaac
    38401 tacattcttt aaagcagttt gttcctctgt tggctcataa tattctccat tactatctcc
    38461 aacttgaaac ttggttaatc caacaagctc tcctagtgca gttgcatttg ctattttttc
    38521 tctaccaatt ttggttaata ttatataaaa tttttcttcc aattatacca cctccgattt
    38581 aggataaact actacatttt ctacgcctgt atcatttgca atagctatat taatcttaca
    38641 attattttca atgcatttaa tgcttaaagg ataaaccttt atttcttctc tacttaacat
    38701 tccagctcca cagtttacaa cagcgctaca ttttataatt ggttcaaagt atggatgaac
    38761 ttttacttct tcactacata ccatacctcc accacaatag accatacttt tatctagtat
    38821 tattggttct aaatagtgta ataagtgagc aggttttact ctttcaatca tatcgctaca
    38881 atctaaaaga gttttatagt cacaattatt tgctataaaa ctcaatacga atgtaaattc
    38941 atcactataa acttttatat ctgtttctga ttttgtatat gcctcacata tactttttat
    39001 aacttcaata gtactagtac ctctgcttct cattttagct tttatattgc tacgtcttgt
    39061 gtcaaaatca agttcttttt tttcaatgca gagtatgtct tcccacatat ctaatcccca
    39121 tgttgcagtg tcaacaaata attggttaaa ggtatcatca atatcttctt taagaatatt
    39181 taattctgta tcgtatgcac cttgtatctc ctctacaatg taatttctat caaatgatgg
    39241 tagtttatca attagcttca ttgattttcc acctcactaa aattaacagt tgttacacta
    39301 ggtattttat cttcattaat agttatatta tctgtacttc catttataag taggttactt
    39361 aaatcgtgta caccagtagt atttataagt attcccatta cttttgtata gattatctct
    39421 cctctaatat ctctaaagta tgtatttata ctttctagga aagattcttt tacattgtct
    39481 aatgtatatc catcttctaa tttcattact gcacttatat ttatttctat tggtaatggt
    39541 gtcacaactg ttatagttgg tccaataggc ttctcttcat ctatatgttg ctgacacctt
    39601 tcaattgttt ctgtatcaac agcttgatta tttttcccaa atatcaagac cttaactgtt
    39661 cccggaccat cccatcttgg ataaacctta acattataga ctccatctac ttccaaagcc
    39721 cattcttcat agtgagcttt atttccactt gtagcttgat ttctttggat tttataaaat
    39781 ctttctttta gttcttcatc tgtctctata tctgtaccac cttcaaaact tcttgtgtta
    39841 gtaatctttg ttactccaga tatattatca actagtttaa attcacaatt tgtaggtaaa
    39901 ttatatttct taccaacttc cagagcttga actgggctat tgtcaccttc ttcactacca
    39961 atagttacat cttttattac tacaaatagt aaatctctat atgatattat tgtgccatta
    40021 tttattacag ttcctttctc tccaataaat tcaacttctc catttgactc tgtacctaac
    40081 tttctatata caccaaattc attgactctt ttatcgagaa actggttata tgtgtcttgt
    40141 ataaaagcca ttttatgtat ctttgaaagt tctagatata tctttgaaag ttctagatta
    40201 tttccagata ccatgttgtt tagaaaagaa ccttctcctt tataaatatc aagattaata
    40261 ttttcaagag ttctattttt tattacttca tatgtctggt cactatacat ttatttccac
    40321 ctccccatag atagtggata cttttacatt tgcactcaat acgtctctgt taaagttagc
    40381 actttttacg tttacttcta atatatatgg atttattaga agggcctctt ttatgaatct
    40441 actagcttca ctttctgtaa gaccttttgt atatttttgt cctattagtt ctgaaagctc
    40501 tgtcccatat tctaaagagt atatctcatg ttcatatcta tttgtcttta tacacttgta
    40561 tatccaaact tttaaggctt cttttttttc aattatttta aagtcacctt tttctaaaat
    40621 aggttcatct ttttcaaaat cccatgccac ttcacgaaag attggcaatt cttctgtttt
    40681 aggtaagata taatcctctg ggacacctat aaaaggaaat attgtactca ttataagctc
    40741 accaacttac ttacaacagc aaatttttcg cctattctaa acattattac catatcccca
    40801 gattcaaaat tatctataaa aggatttttt acttcatgct ggtgttcttg atttgtttct
    40861 gtgtcaaatg tttctagctg tctatcaatc atccaactat ctatcaaaat atcttctttt
    40921 tctaatatga tgttatttgt ctctattttt aaatctggta atttactttt aatttttcca
    40981 ataaaaaaag aaggttgatt atgatacttt ccttcttctc ttattattcc tataaattca
    41041 tttattggat tagccattat atcaccatcc ttttataata ctctcctagc tgtattaaaa
    41101 tctcttctac tacttaactt acttatttta actacatcac ctttttgtgg agcatgtaaa
    41161 aattctccat tacctatata taatccaaca tggctgactg gattatgaaa aaatactaaa
    41221 tctcctgctt gaagattttt ttgttctact ttcttgcctt ttttagattg ttgatttgac
    41281 gttcttggca aactaacatt aactttttta aaacaataga ccattaaacc agaacagtca
    41341 aaactacttg gtccatttcc accccattta taaggtttac ctagatgttt ttttgcttct
    41401 gatattacta ttttagcttt atcagataca ttaccagaat aaccaccaat aataattttt
    41461 ccttttcttc ttccaaattt actagcttct tcaacatttc caaataaaat atctatatgg
    41521 tatgttccat tcttttctat agttattgca ggtcctacgt catttacttt atagactcca
    41581 tcacgaccag aaactcctgt aattacctgt atttcatctc catagtttaa taaaggatgt
    41641 ttatttaaaa actcttttgt ataataagtt tgttcatatt taccaaccat aggagcagcg
    41701 caagtttttt tagatgggtc aagttttttc tttctacaat ctgtatctcc accttcttct
    41761 tttctaggac agtaagctgt aaattctgct gtaaactctt ttcctcctgc ataatcttct
    41821 ccccctaagt tactttcttc cttaggttca tcctgtcctg ctgacttttc atccataaga
    41881 ttttgaaaat taagttcaag ctcaatttga tattctccat tttgccaagt atgtttgtct
    41941 gtatctatat aaaatagtcc tacaagcttt gtataagaat ctttaacttt tactcctcta
    42001 ccagttatac aacttacatc tccataacct ttaagagaac agcttttttc tatcccatta
    42061 aactcgctat caatatctac atcttgattt tcttgttgct gaattacttt ttgcattatt
    42121 acatttactt ccttaaaaat ttcattatct attttttcgc taatcttgct tccatactgg
    42181 tctaccacta ttactttatt ttttacattt tccatgctct ccgaaaaggt ggtatttata
    42241 atattaaatc cctcttcaaa cataacattt aatgtaacag ttcctttttc aataacatta
    42301 aacttatcta aattggcctc tatcatatac tttttctttg tctttttact tgcttctgta
    42361 tatgcactca ttattgtgtc ataaccattt actcctataa acatctttgt atacttgaca
    42421 ttggtctttg ctattgttcc aactgaaagc ctattttcag caaatacttg ctttgctatg
    42481 tcttcaacta acttatcttt aaaattgtat gacacttcac tttgtgtaag taaaaatccc
    42541 atatctttag atacaaaact aatttcatta ctgcttgaat ctttagacct attaattatc
    42601 attcctctaa agagttcttt ctcatctaca tagaaacaga ctgtactagc tattggtata
    42661 tctatttgtt ggaaatttac atcaaatgat gattgtatta ttgaaaactc tagtgtcctt
    42721 gatggagatt tataatcacc tgaccatgat accttgtcta ctatgtcagt tatgtcatat
    42781 atacttccgt tttttatgtg tacccaaatc ttaataataa aaaatcacct gccttttcat
    42841 ttaattctat aagcagttat ttataactta ttatctataa tagttattgt tttggagtta
    42901 cttttggtat ctttattctt ttatactctt ttaaatctaa tgtaaaatat acatctcgag
    42961 tcccatctct ttcttcataa ctaaaccctt caattatgac ttccatattt atatttgttt
    43021 ccgtaattgt aaatcttaat ataaaacctt ccttcatcca tttttctatc ttatttacac
    43081 aatcatatgg tgatggaaaa cctgtatagt cacagaaagt atagtcttta tttggaaaaa
    43141 aacttgatat tgaaatgctt ttaagagcta caccaccaaa agttgctatt tcacctattt
    43201 tgagtatact agaagagttt acagcagctt ttccattaat actaaaggaa gatggaaata
    43261 ctggaaatct aaatctatct tcagcttgtc taagccacat ttccattata agacaccccc
    43321 taactgtgcc aattggatac ttgcaactaa ttttgatgtt atcttttcta tatcagcttc
    43381 ttctctaatc actattgtat cagctaactt agctatgttt atatttccac cattttttct
    43441 gcttctatat tgatttgctt cttgtttagt taaaaccatt tcaccttcat ggagtctggt
    43501 ttgatagtca ttataaggaa catagttcaa tccaaatgca ttattacctt tcttacctga
    43561 ataagcttta ccagaatcac cagcctttgt tccagcatat ggattatctt tgtaattttt
    43621 tttactcttt tccactagat ttaaaactgt tcctgtgatg cttccatttt ttatcttatt
    43681 aatcatagtt tgccaccact tgcatatatc tttaactatt ttacatactt tatctaatat
    43741 gttaaaaaaa gctcccaata ttggctctat aattttccat ccagtttcca acagaggtcc
    43801 taaggtctcc catacagttt tccaaataac acccagtgtc tctataaaat tgtttatttg
    43861 aggtgcatat tcacctaaaa aagtaaaaat tcttgtgata ataggactaa cagcttcaac
    43921 tacaggtccg attagttgga atgctgtagt tactattgta ccaaatatac ttattaatcc
    43981 ttgtgcaatt ggtgatttat tgtttatact atctagtaaa ttatttacta agtttactac
    44041 tggaagtaat gaatctatta atgctgtaaa atcgatattt tgtaatagtg tacttcctgc
    44101 gttaacaatt gcttttggtg cattccctat cattgttgct gcatgatttt caaaacgttc
    44161 ttgcttattt tcaaccatca ttttacgctt ttcagcctca ttagaaattc gatattgagt
    44221 agaatcagaa aactcttttc cattttttac acttctagat tgcatctggt catatttgtt
    44281 gccaataatt atcttaccat tcattacatt gcctatttta ccagccattt tttcagataa
    44341 gccagctaaa ttgcctaatc cactttctat aaaagttttt attttcttta gtccttgacc
    44401 taaaatatca tcaaaaggtt taacaatact cttcaagcca ttgttaatgc ttgtttgagc
    44461 tcctgatatt atacctggta ttgtattact catttcttta gctaacccca tctgctttac
    44521 acttatgcct tccaatgctt catcaaaagt tttataattt tctcctacca gagtattaag
    44581 agattccatg tttccttttg ctgcacttaa gaaagctgct gatacatctt gttcactact
    44641 tgtattcata tttaaagctc taacatctac catagcttga gttatatctt tttctttttt
    44701 gccaccagta atattactaa tcattttagc tttagttccc attgcaacaa catcttcagg
    44761 gtcaaaaggc gtatcatttg ccattcttac taaactgcca taataatcat caacatctat
    44821 ttgattaact tttccagtat ttcttttcat tacactttct agaacaactt tctgattttg
    44881 ttcctgacca agtaaatcaa cacccttttt cacagcgaca cctatagtag caccatttgt
    44941 caaatcagat atcttccctt ttatcgaacc aagtattcca cttgccatat cctttacagc
    45001 tattgcacca ctccatacct tacctgcaaa taatctcaat ttgggaaata ctttacttat
    45061 aatgccactt gccatatctt ttatagctat tacaccactc caaaaagtat ttttaaacaa
    45121 cattaactta ggcaaaattc tacccatagc actactcacc atatctttaa gattaatact
    45181 tccttgataa ggacgtttct ctacttgttt taattttccc aagatactac ttataactct
    45241 cttagtcata tcttttgctt ttattacgat attccaaacc ttgccaccaa tcttttttaa
    45301 atttccaagt aatttatttt tagttttttc tacttcatca cttttagctt taaatattaa
    45361 tataatcata atatttgaat taaactcatt tttcttttga atcaacttat taaaataatc
    45421 aataatcata gctatagtta ttgctagtat cataatactt ccatttaaac ttccaataac
    45481 tcctgaaaca gcatttatgc tagaacttaa ttttgtcata gttgaactta cactactagc
    45541 agtagtttga acttttctta acgaggaatt taaactgttt actctattac ttgcacttcc
    45601 aatagaattt tttatgctac tagccattac attagtggaa agtgaaacag ccacagttgc
    45661 tctcctaaaa ttatcagtta ctgatataat ttgattcatt tttggtgtgt aattatctgt
    45721 aaggtacata tttatacctt cttctctcac atttcccatt acataccacc tcctgtcaaa
    45781 ggacacactc catatccatg tttcattttt tcaacttctt cctcaatttc taaggcgaaa
    45841 aaagcttcaa taagctttaa ttcgccttta tccattgcat aaaaaaggga cggtcttata
    45901 ccttttttct tccaataata gtacattatt gtagtcctgg tatccgtccc tattagtttt
    45961 ttacctcttc tatcactgca tttttaccat aacccataac atcttgaagt attctatata
    46021 agttggtaat ttcacctggt aataataatt ttcttgctaa ttcttttggt gttggagcct
    46081 taaatttatc cattagctcc ttatttttaa ataaaagctt tccatccaaa tcacaaacac
    46141 cttctataat tgtaagcatc tgcatctccg gtaagtctat atccgttttc atattttcat
    46201 caattttaag acaagtattt tgtatttctt catctttact tattgttaaa gctctacaca
    46261 ttactttaaa ttcctgccca aatatagttg ataatctctt aagttcaact atagtactcg
    46321 gtctttctaa gttttctgta tctgaattta aaagtaaatc tactatgttt atattttttg
    46381 ataatccatt ttcactcatt attatttgct cctttatcaa tcaaattcaa tcaactaact
    46441 tttttactta ttaactatct gaatttatta agtatatatt tactaaatat cagtataaaa
    46501 ccaaatataa aactaaatat aaaactaaat tatatcaaga aaatcatact cagtaaatgt
    46561 gaaaggtgct tctatctctc cttttacgcc aacctcccaa tcagctaaag ttaaatcatc
    46621 aaatgctata ttctttactg ctattctttc tgctccataa gaatctgggt catttaattt
    46681 agatatagca acaaacctag gttcagaacc tctctttatc ttttctccaa ttaacttgtg
    46741 cattcttgaa ctaacgtggt atagagttat tgagcctttt cctttatatc ctatatactt
    46801 tgtatcagta cccatttgac ctgctattat aatatcttct tttgtaaatt ccatctttgc
    46861 ttgaaatttc tttacttctg ctactttgtt tccatcaagc caaagttctc cccaagtacc
    46921 actcattaca tttctagctt ccatatttgc catattaata atcctcctat atttctattg
    46981 ataaatctat atcttccata gcatcaagta cttttatttt tgcttttaaa aatactttag
    47041 aacctgtgtt agcttctttt atttcttgta atgtcatata agataaatct actccttttg
    47101 attttaaata cgatttttgt gcttcaaaat ctatttcaac agtagaatca gattctataa
    47161 gtgctgattt ttctaattct tctaaataac tttttatagc tactatcaat aaacatttgt
    47221 tgtcataact gttagtaacc tttcctatat agtcatctat tatcaccttt cttatgtcac
    47281 tatgtataat atctaaagtg tcaactattt ttattttctg gaacatttct cctttttctg
    47341 ctgttaattc agttaaagaa tttactcctc tagcaattct tatagctcct gcttctttaa
    47401 taagtataag ctctccttta ttaacctttg attctgcatc aactttcgtc atcttaggta
    47461 tatcgactac atcactaagt ttagtataag ttactgattg acttaaaggt gtacctgcta
    47521 taagtccagc caccctactt gtaaactcat caacactgta tttcttttct ccaactaaca
    47581 catcttctgt agtaaaatta attatccctt catgatttcc tacaactttt cctaatacag
    47641 ctttaacctt aaccttatct atatctctaa gtttaattat ccaatttttt atagcagtct
    47701 tatcagcttc tactgctttt ggcatacata gataattaaa ttccttagtc tctaaaaaat
    47761 ctaatgcagt ttgaatatct gcttctcctt ctattacata aactaataac ttatttggag
    47821 tgttaacatt tcccatcaaa gctaaattta tatattcttt attttcagca gataaatcaa
    47881 ctggtatatc ctctttttca tgtatttcat taagacctag tgccttagca tctttaagta
    47941 ccattgcaat tattcctcta gctgaacgtt ctttaacagt tgtagctagc tccttaaatg
    48001 atatgttgat acttggtaat cctatagcca tactatcact ctcctaaatt taaattgatt
    48061 tcttccatat tttcatatac tacatcttct tcatatacac tatccagata gtttagtgta
    48121 aatgtaaagt tcaatacata aattgactct tcttttttaa tactttgttc tagctttgtt
    48181 atatttaatt ttctgtctgt tacctttata tttctgttga ataacttatt taattcatct
    48241 gaaacttcat acattctaat actctttgac tgtggttttg gtaagtattt tatagagata
    48301 acaatatctt tgttattcgt atttaacatt acaggtgtac aaataactga tagtatactt
    48361 acaaaaaaac aagacccttc agagccttgc acactttcat catcaataaa aatactttca
    48421 gtaaagtttt ttttgagaat ctcaataatt gtttctaata tttccttgta tttaagcaaa
    48481 taatcaccac actatcacta aatattcttg cttaatctga taatcttact ataaatgata
    48541 taagtcattt ttaaatatat atctcattta ttcctgtcaa gtatcttcta tatttttagg
    48601 taatattatt cttttcaatc tatcacctcc ttcaatatgg atttctttca caataacatt
    48661 atctcacata ttttattgcc tattgttgta atggtgtata taaagtgttg taaaattgtc
    48721 ctatttttta taatatctca ctcaatttaa atatcatctt tttctttaaa ttgtagcaag
    48781 atgttctatc taagtgtagt ttcatagaaa tatatgtcat attgtttttt gtcttactat
    48841 tataaaaaag attaaaaaat tccatttcta tatcatttag acacgttaga gcattttcta
    48901 tcttctcttt ttctatttct atatcttctt tttccatttt taattttctt aatctttcct
    48961 ctctttttaa cacctcattt tccacagaag agcttatgtt atatgtgtta ctagtctttt
    49021 ctgtatatac cattgctcca catccagagt attcattctc acatcttttt atgtccaagt
    49081 taatattctt tatctttata tccaaatact tataattatg taatctttta tctgtttctt
    49141 taaataatat atcttttttg ttttccatag tacttcccct tttttatata cttattatta
    49201 gcttcaaatt tacaataatc tctttagtgc acagtttttt actaatctca caaccttcaa
    49261 gaacatattg ttctataact gtataaaaaa aacttcttct atattttgtt ctaaaatact
    49321 agaaatcttc ttagccataa gaagtccagg attttttatg cctctttcta tttgagatat
    49381 ataatcattt gtaattccag cctttctggc aagttgaatt tgtgtcatac ctttgcattt
    49441 tctataatat ttcaagttat ttttaaacat tgttttatac ctccattact attaagaata
    49501 ttttgttctt cctcacttag attatataga attatttgtt ctttatcaat agattttaga
    49561 aaaaaatatt cttttatatt ttattagttt tattttttag aacaatttgt tataatctat
    49621 acataaggtg gtgttataat gtttgccaaa agattaagag aacttagaaa ggaatttgga
    49681 ttgactcaaa gagaacttgg agaaaaagta ggcgtttccc aaagagtctt agggtactat
    49741 gaaacagaaa acagatttcc tgatgaacac attttaaata aattggctga tgtatttaat
    49801 gtatcagttg attaccttct tggaagaacc ttagttaagg aaaatattga tacagtagct
    49861 gcacatagaa aaaatccaca tgaagagtta cctgaagaag cacaagaaca acttaatgat
    49921 tatatagaat ttttactaaa taaatataaa aaaaaataaa ccatatgatg agcagtctat
    49981 ttttctgctc ttttaatata attcaaaggc aaacatacat tctattttag ggggattttt
    50041 atgaataatt tggataagct ttttgaatta gcttctcaag aagagataat aattcattat
    50101 accacttata ttgcaggtga tttagaagga ctatatataa acaaacatgg tattaaaatt
    50161 atatcattac tcagtaattt aaaacaaaac tcaaaaaaat tgacatccat cttagctgaa
    50221 gaacttggac atcatttcac tagccttgga tactatgtat cctcttataa cgattattat
    50281 acaaaaatta ttatagataa gtgcgaaaat aaagccttaa aatgggcttg tgagttctta
    50341 attactgaag aagatataat aaacataatt aactctggca tcacttgcgt ttatgaaatg
    50401 gcagacatac ttaatgtgga tattactttc tttcaaaaaa gattagaatt tctatcatta
    50461 aaaaaacagt ctttacaact tggaaataat aaatatttaa tattaactaa tttgccgtat
    50521 ttctacatat ttgatccaat ttcataataa aaatagtagt aattttaa
//
LOCUS       DAYATN010000014        49847 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.014, whole genome shotgun sequence.
ACCESSION   DAYATN010000014 DAYATN010000000
VERSION     DAYATN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.014"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            380..2281
                     /gene="cooS"
                     /locus_tag="ACGI8A_000788"
     CDS             380..2281
                     /gene="cooS"
                     /locus_tag="ACGI8A_000788"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HFH0178799.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            2274..2747
                     /locus_tag="ACGI8A_000789"
     CDS             2274..2747
                     /locus_tag="ACGI8A_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0178800.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            2722..3948
                     /locus_tag="ACGI8A_000790"
     CDS             2722..3948
                     /locus_tag="ACGI8A_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0178801.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRILKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(4087..5274)
                     /locus_tag="ACGI8A_000791"
     CDS             complement(4087..5274)
                     /locus_tag="ACGI8A_000791"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0178802.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRIVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            5547..6371
                     /locus_tag="ACGI8A_000792"
     CDS             5547..6371
                     /locus_tag="ACGI8A_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0178803.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GSNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            6500..7765
                     /gene="gluD"
                     /locus_tag="ACGI8A_000793"
     CDS             6500..7765
                     /gene="gluD"
                     /locus_tag="ACGI8A_000793"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HFH0178804.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            7978..8568
                     /locus_tag="ACGI8A_000794"
     CDS             7978..8568
                     /locus_tag="ACGI8A_000794"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFH0178805.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            8899..10032
                     /locus_tag="ACGI8A_000795"
     CDS             8899..10032
                     /locus_tag="ACGI8A_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFH0178806.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDGEIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKGMEEKDVTNSAIVTMIEF
                     TYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGIEYYDSLLND"
     gene            10948..11970
                     /locus_tag="ACGI8A_000796"
     CDS             10948..11970
                     /locus_tag="ACGI8A_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFH0178807.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            12266..13186
                     /gene="pyrB"
                     /locus_tag="ACGI8A_000797"
     CDS             12266..13186
                     /gene="pyrB"
                     /locus_tag="ACGI8A_000797"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFH0178808.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            13225..13917
                     /locus_tag="ACGI8A_000798"
     CDS             13225..13917
                     /locus_tag="ACGI8A_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFH0178809.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            13910..14812
                     /locus_tag="ACGI8A_000799"
     CDS             13910..14812
                     /locus_tag="ACGI8A_000799"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFH0178810.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETSSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            14897..15481
                     /gene="pyrE"
                     /locus_tag="ACGI8A_000800"
     CDS             14897..15481
                     /gene="pyrE"
                     /locus_tag="ACGI8A_000800"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFH0178811.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            15695..16621
                     /locus_tag="ACGI8A_000801"
     CDS             15695..16621
                     /locus_tag="ACGI8A_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HFH0178812.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(16673..17563)
                     /locus_tag="ACGI8A_000802"
     CDS             complement(16673..17563)
                     /locus_tag="ACGI8A_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0178813.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            17751..18782
                     /locus_tag="ACGI8A_000803"
     CDS             17751..18782
                     /locus_tag="ACGI8A_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFH0178814.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            19076..19951
                     /locus_tag="ACGI8A_000804"
     CDS             19076..19951
                     /locus_tag="ACGI8A_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HFH0178815.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            19969..21459
                     /gene="cls"
                     /locus_tag="ACGI8A_000805"
     CDS             19969..21459
                     /gene="cls"
                     /locus_tag="ACGI8A_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFH0178816.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            21652..21936
                     /gene="groES"
                     /locus_tag="ACGI8A_000806"
     CDS             21652..21936
                     /gene="groES"
                     /locus_tag="ACGI8A_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFH0178817.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            21969..23597
                     /gene="groL"
                     /locus_tag="ACGI8A_000807"
     CDS             21969..23597
                     /gene="groL"
                     /locus_tag="ACGI8A_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFH0178818.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTESAVADLPEKEDAGMPGMGGGMPGMM"
     gene            23859..24599
                     /gene="larB"
                     /locus_tag="ACGI8A_000808"
     CDS             23859..24599
                     /gene="larB"
                     /locus_tag="ACGI8A_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HFH0178819.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            24734..25866
                     /gene="larC"
                     /locus_tag="ACGI8A_000809"
                     /pseudo
     CDS             24734..25866
                     /gene="larC"
                     /locus_tag="ACGI8A_000809"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
     regulatory      25926..26027
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            26248..27783
                     /gene="guaA"
                     /locus_tag="ACGI8A_000810"
     CDS             26248..27783
                     /gene="guaA"
                     /locus_tag="ACGI8A_000810"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFH0178820.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(27860..28906)
                     /locus_tag="ACGI8A_000811"
     CDS             complement(27860..28906)
                     /locus_tag="ACGI8A_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0178821.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(28983..29198)
                     /locus_tag="ACGI8A_000812"
     CDS             complement(28983..29198)
                     /locus_tag="ACGI8A_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0178822.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
     gene            29484..29615
                     /locus_tag="ACGI8A_000813"
     CDS             29484..29615
                     /locus_tag="ACGI8A_000813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178823.1"
                     /translation="MNENENMSLYVSKLKEVLKNNSEEYKKIRAKINEIYNLNEKKE"
     gene            complement(29632..30033)
                     /locus_tag="ACGI8A_000814"
     CDS             complement(29632..30033)
                     /locus_tag="ACGI8A_000814"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0178824.1"
                     /translation="MQTLAEIIKEYRSEHNLSLREFSNLCGVSHTYIDKIEKNRDPRN
                     GKAVEPTLDMLEKMSFGLNLTLKELLTKLGKIQPNTQQDDELLKKVNSKEDNEVNELI
                     NRLASLDSDDKNAIKKMIDNAYYKAVNTKKE"
     gene            30219..30410
                     /locus_tag="ACGI8A_000815"
     CDS             30219..30410
                     /locus_tag="ACGI8A_000815"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0178825.1"
                     /translation="MRKESRISQKKFADKIGISRPYLSRIENGKVNPSLEIAHKISLE
                     TGKTINEIFFDFTVNHSKL"
     gene            30650..30787
                     /locus_tag="ACGI8A_000816"
     CDS             30650..30787
                     /locus_tag="ACGI8A_000816"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178826.1"
                     /translation="MQREQTTLRIPEDLHKALIDLSSDIGMPITSIIIIACWLYISKI
                     N"
     gene            complement(30773..31084)
                     /locus_tag="ACGI8A_000817"
     CDS             complement(30773..31084)
                     /locus_tag="ACGI8A_000817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178827.1"
                     /translation="MTTITVRIYTPLNENLEKISYQTGILKSSLILYAINDIIRNSKV
                     NELQSISYKSDDTVRSTLRIPGVLKELLEKTAKENNLSINSLINNVVHSFCISDWLIY
                     L"
     gene            31205..31375
                     /locus_tag="ACGI8A_000818"
     CDS             31205..31375
                     /locus_tag="ACGI8A_000818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178828.1"
                     /translation="MEKVRMTVRLVPKLNQYVQNIAKECGKSKNSIIVDACWEFIEKI
                     KKENKFNVESEE"
     gene            31378..32016
                     /locus_tag="ACGI8A_000819"
     CDS             31378..32016
                     /locus_tag="ACGI8A_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFH0178829.1"
                     /translation="MNNLQIFEKLEFGQIRMAEIDNKPYFVGKDVAKSLGYKNTNDAI
                     LRHCKGVVKHEGFKINGIKIALITEGDVYRLIVGSNLPNAEKFESWVFDEVLPSIRET
                     GQYQAQQHVITELTGTIGDLKGTIKEYKKLCKITCSKKQQYSKYIKNRLDIDKANKEY
                     NQVKARLFLILGVEKWEDIDFDTSNNLIQIIDDCIRVIKSERPYKQLSYFEN"
     gene            32075..32245
                     /locus_tag="ACGI8A_000820"
     CDS             32075..32245
                     /locus_tag="ACGI8A_000820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178830.1"
                     /translation="MKNNISDSRVKYFCKCPYCGFDNEVEVKKGLKPKICCICTKEVE
                     YEKLEQQSTPAK"
     gene            complement(32248..32481)
                     /locus_tag="ACGI8A_000821"
     CDS             complement(32248..32481)
                     /locus_tag="ACGI8A_000821"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178831.1"
                     /translation="MNIGKITKIEATCFDCGTKVTITESKFREVCDNGLPCPMCKEHI
                     SNSNFIAEQALQYNHAVNKLEKELDSCKNIHIY"
     gene            32550..32747
                     /locus_tag="ACGI8A_000822"
     CDS             32550..32747
                     /locus_tag="ACGI8A_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0178832.1"
                     /translation="MAINDNINKILRDRNLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKLADYLDVTLDELVGR"
     gene            32786..32992
                     /locus_tag="ACGI8A_000823"
     CDS             32786..32992
                     /locus_tag="ACGI8A_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178833.1"
                     /translation="MAKAVAKEQLFYRAKDIAKILDVCEATAYKIIGELNKELEKEGW
                     KTFPGRVSVTYFKERYCYKTKKGA"
     gene            33164..33352
                     /locus_tag="ACGI8A_000824"
     CDS             33164..33352
                     /locus_tag="ACGI8A_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178834.1"
                     /translation="MRIIYKNKIYKVEQDKVLFRITYYDEQKNNRKFNNNKKVKRSVL
                     TRDIELVNLYLPAHLKIK"
     gene            33368..33580
                     /locus_tag="ACGI8A_000825"
     CDS             33368..33580
                     /locus_tag="ACGI8A_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178835.1"
                     /translation="MVFNLEKFKVGNAIRISCERFGFEIDCIVVVATEEELNLAYFDK
                     ERGCIEYQALMPEDLRYDDYILERLG"
     gene            33596..33766
                     /locus_tag="ACGI8A_000826"
     CDS             33596..33766
                     /locus_tag="ACGI8A_000826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178836.1"
                     /translation="METAISIAKGQIEGANESIKELKSKENYDKESLRWWEGVKQASE
                     NILEFLEIENKA"
     gene            33848..35800
                     /locus_tag="ACGI8A_000827"
     CDS             33848..35800
                     /locus_tag="ACGI8A_000827"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0178837.1"
                     /translation="MKAILLKSLNIENFKGIKELRIDFNNITNVFGENATGKTSIFDA
                     FTWVMFDKDSKNRSVFEIKPLDKQNKVIRGLVTTVTAVLEVNNKEIKLTKKYEEKWTR
                     KRGESEATFTKNETTYMINDTPIKKSEYVKEVAEIANEEQFKLLTNPYFFSNELNWKK
                     GRELILNVCGDITTEQIIESNKDLTLLITEFEKENNIDKIIKNRKAIKNNLSKEKEEI
                     PVRVNECNKSMYDIDFEEIEAQLKAKKADLEAIEDMLLSGTKANEQILKDKEKIFELK
                     QETQSIKQSASEKGNKKRNELLKEKDELKYNIKNLRNNLVYLERDNELKETLKKRAIE
                     KTTNLRDKWTKKSQETLDLSVIQTECPTCKRPLDLEDIEEKKKEMLDNFNLNKAKELK
                     EIAELGKSKNDDVELFNTEIERLKVDINKTLEEIQEKAVLLDKIKKELESTKSTEIYS
                     ADEEKRLTEISAEIKELEEKINNKDADKNIDELKENKKKLTSEIELLNKELAKRDINK
                     ELLDRKEQLLEKEKALGIELAHQEKILNLCELFIKTKVSLLESNISSKFKNVTFKLFK
                     EQINGGIEETCEALINGVPFSNANTAGQINGGLDIINTLSNHFEVKMPIFVDNRESVN
                     NLIDIDSQVINLIVSNDNPLKIEGVN"
     gene            35801..36718
                     /locus_tag="ACGI8A_000828"
     CDS             35801..36718
                     /locus_tag="ACGI8A_000828"
                     /inference="COORDINATES: protein motif:HMM:NF015776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFH0178838.1"
                     /translation="MAERNITDVVLNRVNELKDSGELVIPPTYSAVNALKSAYLILQE
                     TLDKSYKPVLETCTPISIINSLLNMVIQGLSPAKKQCYFVAYGNKLQLMRSYMGTIAV
                     TKRLSNIKDIKAYCIYEGDKFKVKYNKENAVLEIGEYEPAFENIDIGKIKGAFAVIVG
                     NEGILHTEIMTFTQIERAWLQGVGYSNPKNKVHDNFTEEMAKKTVINRACKMYANTSD
                     DSDLLIGAFNETDRVITSEEIAKDTDKEVEKEISQNANIKSLEMPSQVDNTIFTQTKE
                     KVAINIDEDKPNLAEFEEAIQVELDTPPF"
     gene            36763..37431
                     /locus_tag="ACGI8A_000829"
     CDS             36763..37431
                     /locus_tag="ACGI8A_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0178839.1"
                     /translation="MLQLENETLILECGVSYKEILKGLDFSLENVVGCLVTHEHKDHS
                     KAITELTNNGIDVYSSKGTLEALKIENHRTKIIESEKLFKIGNFKIVPFNTKHDAVEP
                     LGFLINHSSFGNLLFITDSYYCEYNFNNLNHIMIECNYSKDLLDNNKDKIYLRNRIVK
                     SHFELSNVINFLKVNDLSNIKTITLLHLSEDNSDKDLFIREIEKNVGVPVIVAEKGLE
                     IYLD"
     gene            37442..38335
                     /locus_tag="ACGI8A_000830"
     CDS             37442..38335
                     /locus_tag="ACGI8A_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFH0178840.1"
                     /translation="MAKYRILQANFWDDGFVLDLTPEEKYFYNYLLTNGRASQCGCYE
                     LPYKIMEMQTGYNRETVEKLIKRFIEYGKIKYDSTTKEILIINWSKHNFSKSPKVRTC
                     ILKEVESIKNKEFQQYMYRVCIDYGYPIDTVSIDYGEKEKEKQKEKEKQKEKEKQKEK
                     VEPLICEEQEKNELKEFKKLYEENIGVVYPVTAEWLIEISSDVDIRLFKSAIEICAEK
                     MNMNIAYLKGILKKWKDANITTYEQLESYRLQQENKKAKKVVNGQLSKNKFANFEQTF
                     TKYTENELDNIIKKSQDAKFK"
     gene            38393..38740
                     /locus_tag="ACGI8A_000831"
     CDS             38393..38740
                     /locus_tag="ACGI8A_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178841.1"
                     /translation="MGKIWMDAGTFLETKDIGDIECNLREMINKNKQKNLIGRINSKG
                     QGRKSKKVECTNIITGESKIFVSAVEASEYLYFTELHITRLARTGKTTKNGWKVRYIE
                     EVSNGISKCGTSN"
     gene            38709..38846
                     /locus_tag="ACGI8A_000832"
     CDS             38709..38846
                     /locus_tag="ACGI8A_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178842.1"
                     /translation="MVLANVEQVIKLAEKILSKKKCSVNKAIDIAIKILNRYEYEGMI
                     K"
     gene            38843..39292
                     /locus_tag="ACGI8A_000833"
     CDS             38843..39292
                     /locus_tag="ACGI8A_000833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178843.1"
                     /translation="MSLIEYRGYDFENKKWIYSKTIMWSNAVDCLLMLKEDFKWRKVC
                     NVGICSEEWAGNNQEIFEGDILKENYNPHNRSSYGIVKRDFNSIKLYLEWHYLKKFEG
                     EWIEIIDKTEIYHSRGYKVIGNEYENLEEVRKEFLERKEMLENERLS"
     gene            39276..39401
                     /locus_tag="ACGI8A_000834"
     CDS             39276..39401
                     /locus_tag="ACGI8A_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178844.1"
                     /translation="MNVLASLILIVGSFIAGRVYEYRLNLKEFENYDNIGGFKNE"
     gene            39394..39552
                     /locus_tag="ACGI8A_000835"
     CDS             39394..39552
                     /locus_tag="ACGI8A_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178845.1"
                     /translation="MNDRLEMINASVNYIQMICESSNIAIIAEHDRVRILDLETKEKY
                     DLLRSEGD"
     gene            39556..39879
                     /locus_tag="ACGI8A_000836"
     CDS             39556..39879
                     /locus_tag="ACGI8A_000836"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178846.1"
                     /translation="MSKVIQCDFCKEIFEKYNGECIELYKKDCSKGVLGIDKHICPTC
                     YEKFIEGKIEKVENAKRFKDKLIDFLVEHEVCDCCACDCVFDSNDEKKCKAGIREWVE
                     SEVEE"
     gene            39881..40180
                     /locus_tag="ACGI8A_000837"
     CDS             39881..40180
                     /locus_tag="ACGI8A_000837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178847.1"
                     /translation="MAKLIVSIPLTKYLKDITKEWGETYITKTYGGHVYLSDEAPGEV
                     FKKEDDLNYIEFNDSDIVMRLPKEVHQHINLKKGCNKNLKDVLKEIKKIEEKTNI"
     gene            40288..41184
                     /locus_tag="ACGI8A_000838"
     CDS             40288..41184
                     /locus_tag="ACGI8A_000838"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFH0178848.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFKGRRSSLFFAVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLIELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFVVGHFRGRSTRKVFPIERKSRKNLEQLNNPTHSTSGVLVNAVLTPDRVNKRQN
                     GRRIKESGEPMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDSQ
                     LYKQAGNAVTANVVYEIAKRMG"
     gene            41263..41484
                     /locus_tag="ACGI8A_000839"
     CDS             41263..41484
                     /locus_tag="ACGI8A_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178849.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSKEMLKQKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYYCDGGIEI"
     gene            41481..42485
                     /locus_tag="ACGI8A_000840"
     CDS             41481..42485
                     /locus_tag="ACGI8A_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFH0178850.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDIEAFFQKKISKVSR
                     DNDIRTVVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNAILESCDLA
                     ICLYSQKDEKKVAILKLDYNNSYTHSISFEEDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDEYHLKVLDKDAEKEEANSKFVTEFLNATKVKDDKYRTKKFKDTVENWITNVL
                     GNDIKQAEDIRSILNYTLKEKHEIDIKDFVDKSIKDDELKNSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRNIKTDNGFEIKGNLTDFEDPMKYTVRQNQNGSIDIIIKNVNFYNEK
                     "
     gene            42496..42786
                     /locus_tag="ACGI8A_000841"
     CDS             42496..42786
                     /locus_tag="ACGI8A_000841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178851.1"
                     /translation="MENKDNFVLNKLGPASKVMKKVEIDEKEIKSYLKELKECEEVKV
                     FIIRKDGIEIPIVFKDGNMNEWLRKEIEISFDRFKDEIAQVKKALIKVTGEN"
     gene            42806..42973
                     /locus_tag="ACGI8A_000842"
     CDS             42806..42973
                     /locus_tag="ACGI8A_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178852.1"
                     /translation="MTNKEMCKSNNLDEREVCKEFGKEICGSCINDRVDCESKDCDIA
                     YENWLEKNTER"
     gene            43059..43400
                     /locus_tag="ACGI8A_000843"
     CDS             43059..43400
                     /locus_tag="ACGI8A_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HFH0178853.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNLKVCESYIEGKNELYK
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEELIK
                     INSHLELEDLN"
     gene            43526..43933
                     /locus_tag="ACGI8A_000844"
     CDS             43526..43933
                     /locus_tag="ACGI8A_000844"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFH0178854.1"
                     /translation="MKVFLVIDGEPVGKERPRMNTYAKRTYTPNKTKNYEDLIKWLYQ
                     SKVKYYFEGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVIKVVADSLN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDVI"
     gene            44035..44889
                     /locus_tag="ACGI8A_000845"
     CDS             44035..44889
                     /locus_tag="ACGI8A_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFH0178855.1"
                     /translation="MNENINKEITVLGTLEIEGMKFHNIEGGFGEHKKAMLVKDIAEI
                     HNRELKHINELINNNIKRFKTNIDIIDLKVSRSERLGDLKANPFEGLGYEDVGYSKQS
                     FNQSRNIYLLSERGYSKLLKILEDDKAWEQYEKIVDGYFSMRKELNNPLLSASKELQA
                     IFMLDKKQEVLETKIENVNEKLENFMDDAPLFNIECECIVKEVKRVATKSLGGHGSKA
                     YKNKSLRGKVYSDIYHQIKREFGVDSYKAIKRCQLDKVLEIVNNYKLPIVFEEEIRLL
                     NSQLSIVS"
     gene            45041..45547
                     /locus_tag="ACGI8A_000846"
     CDS             45041..45547
                     /locus_tag="ACGI8A_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178856.1"
                     /translation="MSKTKKEFFNATKKQLSNYKQLSANIIKLKNEIQMLKDNSVGDL
                     MKGISYDSVKTGKTNKTSNMIEDAIVNVSDLITEKEIELYEAEIIKSTIDLAIRNLKP
                     IHRQIIELKYIDGLMWQEMVDIVHLEERQLSVRASQAISSISIALFGKKALIEQEPLF
                     ELLDYKLN"
     gene            46300..46473
                     /locus_tag="ACGI8A_000847"
     CDS             46300..46473
                     /locus_tag="ACGI8A_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178857.1"
                     /translation="MATKSILKNVDVRKKAFGRNLVSALENAKNKQEKEVVLSKKCSE
                     VPKDKIKDIFGRF"
     gene            46473..47084
                     /locus_tag="ACGI8A_000848"
     CDS             46473..47084
                     /locus_tag="ACGI8A_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFH0178858.1"
                     /translation="MSGYLIVNLSNMLGELEEEEVKKILSSFSCPLNKDVEDFLKNKA
                     IEFSKQGLASTHLVLTSYKGKPVIVGYFTLANKYFTIKRKTLTNSLARKVVKFGQYNE
                     ELRRYIIGAPLIGQIGKNYSNNYNKLIKGDELLKIACDKIKAVQLDMGGKIVYLECED
                     KPKLIEFYKDNGFVDFGKRSLDKDETDSLDGDYLVQMLKYLKK"
     gene            47161..47787
                     /locus_tag="ACGI8A_000849"
     CDS             47161..47787
                     /locus_tag="ACGI8A_000849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178859.1"
                     /translation="MKKITKLILCSLLVISIASPLQVEAKTTHHSSSHVSIHKSSTKS
                     KSSRPKITKTKKATKTYRTNKSIKKKIKLKKQVNKKTQVPNKQAARKDKKTTTYNYYN
                     SYNSNRYYTTSSSGLSFWDCYMLGHLFNNNNKVSEQDIARELQKQGYSKQETDNILRK
                     ANQNQAKKEEKKKRVMMLIFSVLGALFIIGLGLIIFIKRESKGCNKSM"
     gene            47860..48549
                     /gene="terS"
                     /locus_tag="ACGI8A_000850"
     CDS             47860..48549
                     /gene="terS"
                     /locus_tag="ACGI8A_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFH0178860.1"
                     /translation="MNEKADLAHEDYLKGLKYKEIAEKHNVSLSTVKSWATRYWKQKG
                     CNQSKKVATKKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELMQEISIKDKFD
                     ILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASF
                     LNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDELK
                     ISVDYGDRNDS"
     gene            48527..49780
                     /locus_tag="ACGI8A_000851"
     CDS             48527..49780
                     /locus_tag="ACGI8A_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFH0178861.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSSTHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSKGLEKNIFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
BASE COUNT        20168 a         5107 c         9101 g        15471 t
ORIGIN      
        1 aagtgatgca tagaagttta atatataaga actaatgtat aaaagtgatg cataaaagtt
       61 taatatataa gaatttaata tgtataaaag tgatgcataa aagtttaata tataggaatt
      121 taatatgtat aaaagtgatg catagaaatt taatatataa gaatttaata tgtataaatg
      181 tggtgcatag aagtttaatg taaaaatttg gtgtataaga atttaatata tatatattta
      241 ttttaataat ttatattttg tttagggtat ggtaaatcgt ggtattaaat aagtagattt
      301 taatagttaa attatagtca attataataa agctatttaa tctacttttt taatataaaa
      361 atataagggg gaatttgtaa tggcttgtaa cacttgtaaa atgtgcgaaa gtgcagacaa
      421 gaaattagaa agttttgtag cttcaaagga tgttgaaaca gcatttcata gaacagaaga
      481 tcagaaagtt aagtgtggtt ttggacttca gggggtttgt tgtagactct gttcaaatgg
      541 accatgtaga gtaactccta aatcaccaag aggtatatgt ggagcagatg cagacactat
      601 agtagcaaga aactttttaa gagcagtagc atcaggagca gcttgttatt tacatgtggt
      661 agaaaataca gctaagaatt taaaaaatgt tggaataact aaaggtgttg ttaaaggaga
      721 aaaaacatta aatcaattag cagagatgtt tggaatagaa agtgatgaaa agtatgataa
      781 atgtataaag gttgcagaca aagttatcaa cgatttgtat aaatcaagag atgataaaat
      841 ggaattagta gaaaaaatag cttatgcacc tagagtgaaa aagtggaaag agttaggtat
      901 aatgcctggt ggtgcaaagt cagaagtttt tgatgcaata gtaaaatcat caacaaattt
      961 aaatagtgac ccagtagaca tgctagttaa ttgtttgaac ttaggtatat caacaggatt
     1021 atatggtctt acactaacta atcttttaaa tgatgttatg ttgggagaac cagttataag
     1081 aatggctcct gtaggattta atgtaataga cccagactac ataaatataa tgataacagg
     1141 ccatcaacat tcaacatttg caaatttcca agatagatta aaagatgaag atgttataaa
     1201 attagctcaa tctgttggtg caaaaggctt taagctagta ggatgtacat gtgttggtca
     1261 agatttacaa ttaagaggag aacattatca agaagtattt gctggacatg caggtaacaa
     1321 ttttacaagt gaggctgttt tatcaactgg agcaatagat atagttcttt cagaatttaa
     1381 ctgtactata ccagggcttg aaccaatagc agataaatat aaagtaaaaa tggtttgttt
     1441 agatgatgtt gctaaaaaag cgaatgcaga atacataggt ttagacagaa gtaaattaga
     1501 tgaacttagt aacactttaa tagaaaaagc attggagtct tataaggaga gaagaggaag
     1561 tattgaaata gatataccaa aagaccatgg atttgaacaa tctttaactg gtgtaagtga
     1621 aaagaacctg aaagcgtttt taggagattc atggaaacct cttattaatt taattgcaga
     1681 aggaaaaatt aaaggtgttg cagctgttgt aggatgttca aatatgacag caggaggaca
     1741 tgatgtaaat actgttgaac ttactaaaga attgattaaa aaagatataa tagtactatc
     1801 tgcaggatgt tctactggag gattagaaaa tgttggtctt atgtcacctg gagcagaaga
     1861 attagcagga gaaaacttaa aagaagtatg taagacttta ggaataccac cagtattaaa
     1921 ctttggacca tgtctagcta taggaagact agaaatagta gcaacagaat tagcagctga
     1981 acttggaatt gacctaccac aattaccatt agtgctttca gctccacaat ggttggaaga
     2041 acaagcatta gctgatggag cgtttggtct tgcccttggc ttacctcttc atttagcttt
     2101 acctccattt ataacaggag gaaaattggt gacagaagta ttaacagaaa aattaaaaga
     2161 cttaacagga ggacatgtta tagtgaatcc agaccctaaa tcttcagcta atcaattaga
     2221 agaaataata atagatagaa gaaaaaattt agggttaaag gatgtggaat gcaatgcata
     2281 gaatttttat aaataaagat ttatgtacag gatgtaagag ctgtgtacta gcatgtatgc
     2341 taaaacacaa taaagattat gacatgtaca ctcttgattt agaaaatata gataatgata
     2401 gtagagggca tatagaatta gatagtaaac ataataatcc agttccaatt ctttgtagac
     2461 actgtgatga acctgaatgt gtattagcat gtatgagtgg tgcaatgcat aaagatagtg
     2521 aaagtggaat cgtttcatat gatgaagaga aatgtggttc atgttatatg tgtgtaatgt
     2581 cttgtccata tggattatta aagccagatg atagaagcaa gcagaacata cttaaatgtg
     2641 atttatgtaa agatgaggag tatcctagat gtgtagctaa ttgcccaagt ggagcaattg
     2701 aattacagaa ggaggaaaat gatgaattat gtagtgttag gggctagtgc agctggtata
     2761 aatgcagtaa aaacattaag agaattagat aaagattcaa atatagtagt aatttctaaa
     2821 gatgaaaacg tatattcaag atgtatgttg catcacgtta tatctgaaca cagaatttta
     2881 aaacaaataa attttgttga tgaagatttt atggagcaaa ataatgtaaa atggattgca
     2941 ggtaagactg ttaaaggtat agacataaat aaaaaggtag ttcagacaga agacattact
     3001 gtaaattatg ataaactttt aatagctaca ggtgcatctt ctgctattcc tcctataaaa
     3061 aatttaagag atggaaattt tgtatattct gtgagaaaca tagatgatat ttataaaata
     3121 aaagaaaaag cacaaaattc taaaaatgta gttataatag gtgctggttt agttggtata
     3181 gatgcattag tgggattatt taaatatgag cagttaaata tttctgtagc ttttatggaa
     3241 aagtacattt tagatagaca attggatgaa tatacagcat cagtgtatga aaataagttc
     3301 aaagaaaaag gtgtaaaatt ttacccaagt gcatcaatac aagaaattgt attggataac
     3361 agtaaaaatg ttacaggtgt tgcattttca aatggtgaag ttttagatgc agacatggta
     3421 attgttgcta caggggttaa accaaatgct gactttttag atggaacagg aatagaatat
     3481 gatagaggta taataataga tgatatgtgc caaacaacac aaaaagacat atatgcagca
     3541 ggagatgttg taggtaaaaa tgctatttgg cctctggcag taaagcaagg aattgtagct
     3601 gcctataata tggttggaaa agataagaag atagaagatg aatttgcttt taaaaacagt
     3661 atgaatttca tggatatacc aactatttct ataggaatga atacacctgt agatgatagc
     3721 tataaagtgc taactagaca tggaattaat gattataaaa agtttgtttt taaagataat
     3781 gtaatctatg gagcagttat acaaggagat atttcatatg taggtgtatt aacttattta
     3841 ataaaaaata aagttgaaat atatgattta gaaaatagga tatttgatat atgttatgca
     3901 gactttttta atataaaaga aaatggagaa ttttgttata gtgtatagta tatacgtgga
     3961 gtaaggttta taaatacagt aaatcaataa atagagttat tctaaaataa gaaaaatgac
     4021 aaatcttatt tagaaatagc tctatttttt gtaattaaat ttaaattata ttcaatttgt
     4081 aaaatattat tttgaacaat tatacatata ctctctagtt agaggtaaat cattttttat
     4141 acctttagtg aaaagtattt gatgtatatc tataatacca gtgttgaaag cagcagcaca
     4201 agaacaaagg tataaatccc acattcttat aaacttatca tcaaacattt ctttaatctt
     4261 tggaagattc tcagagaaat ttttttgcca gtatagtaag gtttttacat aatgtaatct
     4321 tagactttct acgtctatag tatgatattt gtaatcacta caaatatgta tcatttctct
     4381 taggctagga ataactcctc ctgggaaaat atattttttt atccaagggt ctccataaga
     4441 ttcctgcaat ccacttatat agtgaagtaa aaacaatcca ccatctttta aaacagcatt
     4501 tacgttttta atgaaaagtt catagttttc tctaccaaca tgttcaagca tcccaacgct
     4561 tacaattcta tcaaacataa ggtctgattt ttttaattct ctatagtcca taagtcttac
     4621 ttcaagatag tcttgaaggt tctcttcttt aattcttgcg ttaaattcct taaattgctc
     4681 ttcgctaagt gtaattccaa cgccgtgaat tttatatttt tttgcagctt ctattagtaa
     4741 aaatccccaa ccacaaccaa tatcgagaag gctcatacct tcctctaaat ttagtttagt
     4801 taatatgtgg tgaactttat tgacttgagc atcataaaga gtatcattat catttctaaa
     4861 gtatgcacaa gaataactaa gtgtcttatc taaccataag ctataaaaat cgttgccaat
     4921 gtcatagtgg gcatgtattt ctttcttttg atgtctcatt gaatttgatg agtgcaatag
     4981 atgttttaca gctgaattat caatattgaa tttgtctatt tgttttaaaa gtagatttaa
     5041 agttaagaat aaatctccct caacttcaat gtctctattt atgtaggctt ctccaagtgc
     5101 taaagatgta ctttttaata gttcagactt gctaatatca ttatttacta taactttgaa
     5161 ctgaggggag ccctctccaa ttagaaattc ttcctcatct ttaagcctaa ctagaaatgg
     5221 tgtttcacca aatttctcta aatagtcttt taaaaaattt tttgtaatat tcatattacc
     5281 agctccttct aatgtaaatc cagtaagttt ccctgcttaa aatatattat agtccttttt
     5341 ttaaaaaagt aaaatttttt caataaattt tttatttaaa ggaaatttat tttttataaa
     5401 gaaatttcat gttgtagtat attttttgct tataaatttt ggtactggat ataatattaa
     5461 actgttaatg atataataaa tttaatctat aaagtaaata aaattttgta ataaatagtt
     5521 aaagctattt tataaggaga tatatgatgg aagtatatgc tttggttggg tcaagtggca
     5581 cagggaagag ctttaaggct ttagagttcg catatgaaaa tgatatagaa tatataattg
     5641 atgatgggat tttaatatac aaaaacaagg ttttagctgg gatatcggca aaacaagcaa
     5701 atactactat agaagcagtt aaaagggcta ttttttataa tctggagcat agacaagaag
     5761 ttagagaagc aattaaaaaa gaaaatataa gaaaaattct tattattggg acatcaaaaa
     5821 aaatggttaa tcaaattgta gagagattgt caattggaag ggtttgcaag tttataaata
     5881 tacaagatat aagtactaaa tatgaaatag aaatagcaaa acaagctaga aaagaaggaa
     5941 atcatgtaat tcctgttcca gcagttgaga taaaaagtat ggcaagtggt cttagtataa
     6001 attctttgaa gagacttttt agaaaaggaa gtaataggaa tatggtagtt ttagaaaaaa
     6061 caattataag accaactttt agttatatag gaaagtttta tattagtgct gatgtaatta
     6121 aacagattat tgaatatgaa gtatacaact ttggaagcat tgatagaata aataaaatca
     6181 atatagagaa ttgcaataat tttatgaata tttttgtaag tataaatatc aatgatttag
     6241 aaactataaa aaaaattgaa gacatacaac agacaataaa aaagtctatt gagaaaatga
     6301 ctttagttaa tgtacaaaaa atagatatta acattcataa aattaaaaaa ataaacttcc
     6361 tccaatgtta aaaaataaac tgagaaaatg atatactaat ttgcaataaa gtgtgctata
     6421 attaaactgt aaatttagga aatggttttt tctaaatgga gtatacataa ttataacgta
     6481 tttataaggg ggctttcaaa tgtcaggaaa agatgtaaat gtcttcgaga tggcgcaatc
     6541 tcaagtaaaa aatgcatgtg ataaattagg tatggaacca gcagtttatg aattattaaa
     6601 agaacctatg agagttatag aggtttcaat tccagtaaaa atggatgatg gttctataaa
     6661 aacttttaaa ggatttagat cacaacataa tgatgcagta gggccaacaa aaggtggaat
     6721 aagattccat caaaatgttt caagagacga agtaaaagct ttatctatat ggatgacttt
     6781 caagtgttct gtaacaggta taccatatgg tggaggtaaa ggtggaataa tagtagatcc
     6841 atcaacttta tctcaaggtg aattagaaag attaagtaga ggatacatag atggaatata
     6901 taaattaata ggtgagaaag ttgacgttcc tgcaccagat gtaaatacta atggtcaaat
     6961 aatgtcttgg atggttgatg agtacaataa attaactgga caaagttcta taggtgttat
     7021 aactggtaag ccagttgaat ttggtggttc tttaggaaga acagctgcta ctggatttgg
     7081 tgttgctgtt actgcaagag aagctgctgc taaattagga atagatatga aaaaagctaa
     7141 aatagctgtt caaggtatag gaaacgttgg ttcttataca gttcttaact gtgaaaaact
     7201 tggtggtact gttgtagcta tggctgaatg gtgtaaatca gaaggttctt atgctatata
     7261 caatgaaaat ggtttagatg gtcaagctat gttagattat atgaaagaac atggtaactt
     7321 attaaacttc ccaggagcta agagaatatc tttagaagag ttctgggctt cagatgttga
     7381 tatagtaata ccagctgcat tagaaaactc tataactaaa gaagttgctg aatctataaa
     7441 agctaaatta gtttgtgagg ctgctaatgg accaactact ccagaggctg atgaagtatt
     7501 tgctgaaaga ggaatagttc ttactccaga tatattaact aatgctggtg gagttacagt
     7561 ttcttacttc gagtgggtac aaaacttata tggatactac tggtcagaag aagaagtaga
     7621 gcaaaaagaa gaaatagcta tggttaaagc atttgaatct atttggaaaa tcaaagaaga
     7681 gtacaatgtt acaatgagag aagctgctta catgcattca attaagaaag ttgctgaagc
     7741 tatgaaatta agaggatggt actaagattt actcttaata aataatagga tataattatt
     7801 taagatataa aggggctgta catagtacag cccttaaatt atgtggctta caatgaagta
     7861 agctattttt gttttaactt ataaaaaact tgataaatta tgtaatttag aatgtatttt
     7921 ttaccaccac atgattacaa attagaagag ttagattaga tttttagaca tgaacttatg
     7981 cattttaaaa gcaaagacct taatttaaag tatctagtat tatttttaaa aatagtatat
     8041 tggtttaatc catttatata tataatggaa aaatatatgg atttggattg tgaactttat
     8101 tgtgatgaga gagttttaaa aaattgtact gtacaacaaa agcaagaata tgctctaact
     8161 attgtaaatt caatgaaaag aagtacaaat ttttcaaata aatttgtagc tggattaaat
     8221 aaacaatctg atattagaaa aagagtaata aacatgttca gtgaaaaaca taaaaatggt
     8281 atcttaaggg ctttgattat aagcttgttt tcttctatta cattttttaa attcgattca
     8341 actagtatta ttgaagagaa tgatttctta gagaggtcaa gatatacgtt tgaaggaagg
     8401 caagaagaca tgtttgtaac aatagatttt atatatgcag atgtacctat aaaacttaga
     8461 aaagagcatg aagaaaactg tagagtcaat ggaatagtac caaaagattc agattcaata
     8521 gaaattattc ctagatttta taaagattgt atggatttaa aaaaataagt tagacttaat
     8581 attgatgttg atattatgaa ttttttatat ttaagcaatt gaaaatttta ttatagattc
     8641 aattttatat gtgtttaagt atgaaattta tagatagttg aaataattaa atatgattga
     8701 tgcataggaa tcattactaa gctataatta attataaggg agtttaaata aattatgagt
     8761 tacacaatta taaaatgata aaaaatagtt atctgttaca aaaaatgtat ctttttaatt
     8821 aataaagata atatgaaaac ttaaaaggca aataaactta aacataaaat actcttaaat
     8881 aagataggag aaaaatgtat gtctaacttt attctaaatg ggatttttag aacagttata
     8941 gtaagtagtc taagcattct tatgatattg attttcaaga aaaatgtatt taaaagattt
     9001 agcaaaaaat ttaattatta tatatggatg atagttgtta ttaaattatt tttaccattt
     9061 acatattata cttttacaat aaatatcttg agaagtaaaa aacacattaa tattaataac
     9121 ataaatttag agggatttaa taatgtttca actataagta ataatattat attgtacact
     9181 tggattataa ctgtagtagt ttatttaatt tacactatat ttaaatatat aaaacttaaa
     9241 aatttaataa atgacttatc ttatgatgtt gatgatggag agattgtaaa tctttataaa
     9301 aatatattag aagaatttaa tataacaaaa gatataaaac tcaaatactc ttatgaagtt
     9361 gaaacaccag cattttttaa ttcatgtgtg cttttgccac cacatgagta taaattgaaa
     9421 gagttagact ggatttttag acatgagctt atgcatttta agagtaggga cctttattta
     9481 aaatatgtaa tcctgttttt aaaaacagta tattggttta atccatttat atatataatg
     9541 gataaacata tagatttaga ttgtgaactt tattgtgatg aaagagtttt aaaaaatcga
     9601 aattcagagg aaaaaaagga ttatgcttta acaattctaa atgcaatgag aaaaggttca
     9661 aatacttcaa ataaatttat agctggactc cataagcaat ctgatattaa aaagagagtt
     9721 atgaatatgt ttaatgaaaa gtacaagaat ggtatattaa tggcattaac tttatgttta
     9781 ttatcttcta ttactttttt gaaggttaat tcaacgagta ttgttaatct taatatattg
     9841 ccaaaaggaa tggaagaaaa agatgttact aatagtgcaa ttgttacaat gatagaattt
     9901 acatatgcag aagctcctaa aagtatgaga gaagaacata aaataaattg tgaattctta
     9961 ggtttaatac ctaaagactc tgataaaata ggtgttggta tagaatatta tgacagtcta
    10021 ttgaatgatt aaataaatat tattttaaca gatattaatt ataaagtttt gaatagtaat
    10081 ttgttgagaa ataaatatat ccttataaaa atagatactt atataccaag aataaaaaat
    10141 agtcaagata gtacacaagg atatacttat aaagaactca aataagtgaa ataaattatt
    10201 aattataagg tttataattt taaaaaatta gataaattca ggtgtaaaat actataaaac
    10261 acaattcgga aaagtgtatg ttgaacttca tttaaaatgg aatttcaaag ttgctatagt
    10321 aagtagttta agcattcttc ttatattaat ctttgagaat aatgtattta aaaaagttag
    10381 taaaaaattt aactattgta tattaatgat agttgctatt gatatattat ctaaaaaaat
    10441 taagtatgaa aagattactg ctaggacaat cttttcatac ttaattttta atatttatat
    10501 tatccaaaat tttcatcttt ttaacttaga atttgagaat cactattagg aaataggctt
    10561 tgatttatgt agggttaaaa atttcacaga atatctaact ttatactaaa tccatcaaaa
    10621 aatatacgcc atataaaagc gcataaaaaa acacataaaa caatatttat gcgcacttta
    10681 acctcgaatg gtacaaaatt ctttactttt acagacccat attatataat taagtaaata
    10741 atgtatattt aggtgacata ttggataagc taatttattt attttattaa tataatgata
    10801 ctataaaagt tatataaagt ggtttacaaa aaagttacag atagagcttt tattaatgta
    10861 ttgtgtatta gtattttatc cttaggtaca cttcacctac aaaatagaat cataagataa
    10921 ttatgggaaa attttaggag gtttatcgtg aagaaaaaga tattaatacc agtatttgca
    10981 tcagttatgg cattatcagt aagttcaatt gtaaatgcag atgaagtaaa tgattcatct
    11041 caaaataagg atgataaaac aaatactgaa ttaaatttag gtgaatataa agaagtaaaa
    11101 tataaagttg caaaaataaa agatggagta gctataaaga taagagaaga aggtcaagtt
    11161 caaaacatag cttattctgg agatgaattt acggttttag gaactcaagg agaatgggtt
    11221 aaggttaaag ttgaagatgg cgaaggttgg ttagctacta gatatgtaga tatatctgaa
    11281 ggtgtaggat atactaacgc tgataaagta aacttaagaa aagataagag tgaaagttca
    11341 gaagtaattg aagagctgga aaaaggaagt tctttattgg ttttggaaga taatggagat
    11401 tggttaaaag ttaaagatgg ggaaacagaa ggatatgtaa aatcatctta tatatctgat
    11461 aaagctcctg taatagaaga gccacaagta aacccaagta ttgaccaaaa tgtagattct
    11521 acaaataata atcaacaaaa caatgcaaat actaatcaac aaaataataa ttcaaatgtg
    11581 ccaacagcta actctaatgc agttcaagct gtacttaact tggcatattc taagcaaggt
    11641 tgtccatatg tttggggagc agaaggacca aacacttttg actgttctgg atttacacaa
    11701 tatgtgtata gaaatgcagt aggaaagaat ataccaagaa catctaaagc acaatctaaa
    11761 tatggacaaa ctgtaagtaa agcaaattta caaccaggag acttagtatt cttcacaact
    11821 aatggttctg gttctgttag tcatgttgga atatatgtag gtgggggtaa tatgatacat
    11881 tcaccatcaa caggaaaaac agttagtgta actagtataa attcatcata ctatacagca
    11941 agatttgtta cagcaaaaag aatattataa tcggtttgta atatgacaaa ttgataaata
    12001 cataaatata tataaacgct ctatattata tttaatttaa tcatggaaac aaaaaatgat
    12061 tatattttaa atgtactata gagcgttttt gtatcacttt aaatctaatc aagattcaag
    12121 ttaatatgac tgttaaatag gagccttttc atatgatgat tatttgcgca atcccttgac
    12181 aggatagggc aaaggtggta agctactaac agtacaaaaa tacttttaat tttagttcag
    12241 agaggctaaa aaaggaggac gaaagatgtt aaaatcaaga aatttaatcc agccagaaga
    12301 cttttcaata gaagaaatag atgaaatatt agaattagct caaaaaataa ttgacaatcc
    12361 gtcaaaatat tctcgtatat gtgaaggaaa actattagct acattatttt atgaaccatc
    12421 aacaagaaca agattaagct ttgaatctgc tatgaacaga ttaggaggac gtgtagtagg
    12481 cttttcagag ccaaattcat cctctgcatc aaaaggtgaa actctaggag atacaatgag
    12541 gatagtatct ggttatgttg atataatagc tatgagacat cctcaatcag gtgcagcttc
    12601 agaagcagct agatatacgg aagtgccatt tataaatgca ggagacggca aaaaccagca
    12661 tccaactcaa acacttacag acttacttac tataaaatca ttaaaaggaa ctttagaaag
    12721 ccatacaata ggattatgtg gtgatttaaa atatggaaga actgtgcatt ctttggttaa
    12781 ggctatggca agatataaaa atacaaagtt tgtatttata gcgcctgaag agttaaaaat
    12841 gccagattat ataaaagaag ctataaaggg acatgcatat tatgaaacta ataatttgga
    12901 tgatgttata ggaagcttgg atgttttata tatgactaga gttcaacaag aaagatttga
    12961 agataaatca gagtatgaaa gacttaaaaa ctattatatt ttaaataaag ctaaattaga
    13021 aaaggcatct aaagatatgt tagtaatgca tccattacca agagtaaatg aaatagacat
    13081 agatgtagat agtgatgata gagctgtata ttttaaacaa gctaaatatg gaatgtatgt
    13141 tagaatggct ttaataataa aacttttagg tataaatgaa gactaatagt tatcatataa
    13201 tagtaaaaca aaggagattt tgggatgtat aaaattctgg aaaatatata tataggcgaa
    13261 gatatgtata gaatgaaagt aaaaggtaat tttgaaggta aaatgggaca attctatatg
    13321 ttaagagcat gggatactta tccagtactt tcaagaccta taagtataca tgatatagat
    13381 gaagaaggga taacttttct ttataaagta gtaggagaag gaactcaaat tttgagtaac
    13441 ctaaaggtaa atgataatat aaaattagaa ggcccttatg gtaatggata tgctaaggtt
    13501 gatggaaagg tagctctagt aggaggagga atcggtgtag ctccactata tttggttgct
    13561 aaaaatataa aaaattgtga tgcatatctt gggtttagag aagatgttat attagaagat
    13621 gaatataaac aagtatgtaa taaagtctat acaacagtag gaaatacttt tgtaacagat
    13681 ataattgatg ttgagaaata tgattatata ttaacatgtg gtccaactcc aatgatggaa
    13741 aaattagtta agatggtaga aggtactaaa acgagaatta tggtatctct tgaaaaccat
    13801 atggcttgtg gagtaggtgc ctgtttagta tgtacgtgta aaactaatgg aggaaataaa
    13861 aagacttgta aagatggtcc agtgttctgg ggagaggacg tgatttttaa tgggtaattt
    13921 aagtgtgaaa tttggaagtg tagaatttaa aaatccagta ataatggcgt ctggaacttt
    13981 tggatttgga aaagaatata atgagattta tgatatacaa aaacttggtg gtataagtag
    14041 caagggtctt actttaaata aaaaacctgg taacaatggt atgagagttc atgagacatc
    14101 atccggtatg atgaatagtg ttgggcttga aaaccctgga gttcaaggat ttatagacta
    14161 tgaattacct ttttttagta aactagactt agtcagaata gctaatgttg gaggtgggac
    14221 attagaagac tacttattag gggttcaaat gttaaatgac aagcctatag acattattga
    14281 attaaatata tcttgcccaa atgtcaaagc tggtggaatg gcttttggta taaaaaatga
    14341 agtggcaaga gaagttgttc gtgaggtaag aaacataacg aaactgcctt tagtaataaa
    14401 attatcaccc aatgcagaag atattgtagg aatggcaaaa gtttgtgaag aagaaggtgc
    14461 tgatggagtt tcattagtta atacttttaa agcaatggca attgatataa aaaatagaag
    14521 accagtattt gaaaatgtat atgctgggtt atctggacca gctataaaac ctatagcttt
    14581 gagaatggta catgaagtgt gtaaaaatgt aaatatacct gtaatgggaa tgggcggaat
    14641 aactaaagcc acagatgcta tagagtttat aatggcagga gctacttgta tccaagttgg
    14701 aactgcaaat tttatgaatc ctagaatagg tattgaaata atagatggaa ttaatgaatt
    14761 tatggataga gaaggaataa aaagtttaga cgaaataaga ggaattatat aaggtagaat
    14821 aaatagtatt ttaaagataa aaaatataga ttaaataaac aataatttta taacattaac
    14881 atggaggaaa aataatatga gtaaagtaga tgtagtggat atattaaaga aaagtgatgc
    14941 attgttggag ggacattttt tattatcttc aggaaaacac agtaatagat acgtacaatg
    15001 tgcaaaagta ttaagatttc cagaatatgc tgctgaggta ttaagtacag ttgttgaaca
    15061 aataaaagac ttagatatag acttagtagt aggaccagct atgggtggag taatagtttc
    15121 ttatgagtta ggaagacaat taggaaaaga agctgtattt actgagagaa aagacaatac
    15181 aatggagtta agaagaggat ttgaagttaa aaaaggagca aagataataa ttgctgaaga
    15241 cgttgtaact actggtaaat caactatgga gacaaaaaga gtattagaag ccttaggtgg
    15301 agaagttgta ggtgttgcat gtatagcaga tagaactaat catgatatag gtatgcctat
    15361 atatagtgct ataaaacttg atattcaagt ttatgaatct gatgagtgtc ctttatgtaa
    15421 ggaaggaaaa ttaccagttg ttaagcctgg aagtagagag ttcaaggaat tagggatgta
    15481 ataaaaactt aattatttat agtgttactt aaaaaatgta aattttttag agtaaaaatt
    15541 tgtaatataa catataactt aaaaataaaa agcagttagc cttataaatg aaggttagct
    15601 gtttttttgt taaaaatata tttacaaaat aagagatagt agcagtacaa tataagtata
    15661 aggtaaaagt attatatatt agggaggggt aagtatgaaa aatcaattaa atgaaaatca
    15721 taaaaaggtt gtattttttg gagtaggtgc tgtaggagct acttttgcag aacaattttt
    15781 taactctaaa tatgatttta aaattctttg tgataatgaa agaaagaaaa gatatttaga
    15841 agaaggattt ataataaatg gaaaaaggta tgattttgat tatgtaacta aagatgagta
    15901 taaacaagag gctgatttta taattatagg tctaaaatat aataatttaa aagaaaatat
    15961 aaaagaatta gatggattag ttggaaaaaa tacagttata atgtctctgc taaatggagt
    16021 tgatagcgaa gagataatag gagaaagatt tggaattgaa aaaatggtat attcatatgt
    16081 taccaatata gatgcaaaga aaatcaataa taatattata cacactacta atgggataat
    16141 tgtatttggt aacaaagata atagtgaaga tagaaaaact aatataataa ccgaagtctt
    16201 tgatgatgta aatatagaat atactttatc aaaagatatt caacgagata tgtggtggaa
    16261 gtacatggtt aatattggtg taaatcaaac ttcagctata cttggtgcac cttatggagt
    16321 ttttcagagt tctgagcatt taagagaatt agcaaaatct gcaatgaggg aagttgttgc
    16381 tatagcacaa gcaaaagaca tatctcttac agaagatgat gtggaacatt cattacatag
    16441 aatactagaa cattcaaaag aaggaagaac atcaatgctt caagatgtgg aagctcatag
    16501 acttacagaa gtagatatgt tttctaagaa tatctgcaaa cttggaaaaa aatataacat
    16561 acctactcct ataaatcaga ctttctttta tatgataaaa gtaattgaaa gtagatttta
    16621 aagggtcttt gtaagaaata ttgataatta ttgattttaa attttattaa tgttatatac
    16681 tatttaagtt actaaatatt tttaactctt caatgaaaat agttgctgct tttgataaag
    16741 ttatatcttt tggaaatata tatgagaaat gtctgttgta gctgcttcca agctctatga
    16801 cagacagctc attgtttaga acaggtaagc ttgtcactag attagagact attgtaattc
    16861 ctaaattatt tctaacagat tctttgacag cataattact tccaaaaacc atcatacttt
    16921 ttggaataat ttctttaaca cttaaaaaca tatctaaata atctctggtt ccagaaccat
    16981 cctccctgac tatccatttt tggttttgaa gtttatcaaa actaaaatct tttaataaat
    17041 gactcttgta tggaagtgct aaaaccatct tatcttcaaa aaaatattct tgaataaatg
    17101 aaggggaaga gcaagtgcct tcaataagac caatgtctaa aatataatcc ttaacatgag
    17161 aagatacgat tgatgtattt tttataaaaa cttctacatc tatgtcagga tactttttag
    17221 aaaataatgc tagaaaattt ggtaaaatat actctccaat agttaaactt gcaccaatct
    17281 ttaagtgacc agttatagca tctgaaactt ggcttacatc atgataagta atatttaaaa
    17341 gatttaatat ttctttagct cttttgtaca agatttgtcc actttctgtt ataaaaatgg
    17401 ttttttgttt aatagacctg tttataagca caactttaaa ataattttct agatttttta
    17461 tgtgtttact tacacttggt tgtgatagat ttaaatattc cccagcttta gtaaagtttt
    17521 tgtattcaac aacagctaca aatgttttta attcttcaaa caattttttg tctcctttac
    17581 ctaatgaaat tttacatttt ttatatactt tctacagata attatactat atataataac
    17641 cattaaaaac attaatcatt acgatattat atatttattt tacttatggt tattacaatt
    17701 atatactatt gctatgaaga aaactatatt aattataagg taggtatatt atgttaaaga
    17761 aaaaaagcag tttgacatta aaaactataa gggaaatatt accaggactg tttgtatcag
    17821 tattagtagg ttatattagt atatttattt ctattttaat tcctaaggta ggggcagcat
    17881 caatttctat atttttaggt atgtttgtag gaaacctatt tttaaatcaa aaggtatttc
    17941 aaaaaggtta taaattttca gagacagatt tgttatcata ttcaatagta ctattaggag
    18001 caacactcag tgtatctacc ttaatagact taaaagtttc aggaatttta tttataatat
    18061 tgcaaatgac aataacaata atagctgcac tttatatagg taaaaaacta ggatttgaag
    18121 aaaactttag atttctaatg gcaagtggaa atgcagtatg tggttcttct gcaataggag
    18181 caacagttcc agtggttaat gcgacagata aagaaaaggg aattgcagta actattgtaa
    18241 atgttactgg tatatttctt atgtttttac ttccaataat atctcaattt ttatatagcc
    18301 atgaacttgt aaaaacttca gcaatgatag gtggaacttt gcaatcaatt ggtcaggttg
    18361 tagcaagtgg agcaattgtt ggagaaaatg taaaagattt ggctactatt tttaagatag
    18421 taagagtaat atttctagtt gttgttgtat tagtatttgg acatatgaaa aatcaatcta
    18481 atagggaaat tcttgaagaa gaaaaggatg agataaaaaa gaaaaaagtg actataccat
    18541 ggtatgtaat tggattcttt ataacttgtg cacttttttc aatgaacatt ataccaaaag
    18601 aagtatctgt gttgtgcaaa gaaataagta acaaactaga aataatagct ttagcagcaa
    18661 ttggattaaa ggttaatgta aaagatttag tgaaacaagg gaaagaagtt tctttatatg
    18721 gtttatttgt aggtatagta caagttgtat cagctgtagt tttaataaaa atatttatat
    18781 agcataaaaa tagtatttgt aatattaatt tgattaacta aaataatatg atactaataa
    18841 tttataatat aaataaaaaa tgaatttcga taagtttaag tgggtataaa cttatagaaa
    18901 ttcatttttt atttaaggta aaaatttaaa gtaaaatatt attttacata ataataacct
    18961 tataaatttt gaaattactt atctttgtgt taaaataagt aaaatgcaca tattagagct
    19021 aagtttttaa gtaaataaac aaaatgaaaa tataaatatt aaggagattt ggtatatgaa
    19081 tgtttatgaa aaaggtaatg gagttatact agaaggagta acagattttg acccagtaca
    19141 tatatttgag tgtggtcaat gttttagatg gcacaaacaa gaagatggct catacactgg
    19201 agttgcaaag ggaagaatat taaatgtcaa aaaagagaat gataaaatat atttaaataa
    19261 tactaattta aaggagttta acagcatttg gtataattac tttgatttag gaactgatta
    19321 tacagaaata aagaataagc taaaaaatat ggatgaatat ttaaataagg ctactgaatt
    19381 tggatgggga ataagaatat taagacaaga tggatgggaa atgcttatat catttataat
    19441 ttcctctaat aataggattc ctatgataca aagagcgata gaaaatttat caagaaagtt
    19501 tggtaagtat ataggtgaat atgaaggtaa tgaatactat gcattcccaa caccagaaga
    19561 actaaataag gcctcacaag aggaaataag agcatgtcaa acaggtttta gagataagta
    19621 cataaaaagt actacacagg cagttataga gaataatgat gaggtatctg aatatacaaa
    19681 tttaagtaca gaggattgta gaaaagagct tttaaaattt aatggagtag gaccaaaagt
    19741 atgtgattgt attgcattat ttggtatgca aaaatatgat tcttttccag ttgatgtttg
    19801 ggttaagaga gttatgcaag aattttatat agatgaagac atgagtttac ctaaaatgag
    19861 aacttatgga atcgataaat ttaaagaaat gtctggattt gctcaacagt acttatttta
    19921 ctatgcaaga gaactcggta taggaaaata aattaagggg gagaaattat gggagtaata
    19981 ggaacaatat ttctgttcta tttaattata tcatatctag ctggagccat aatttctgtg
    20041 attatattgc ttgaaaatag agaccctgca aaaacaatgt cttggctatt gatgtttatt
    20101 atatttccag gtgtaggttt aatgatatat gctatttcag gtagaaatat tagaaaaaga
    20161 aaattattta agactcaaaa actagctaat aatataaaag aaaagaaact atttgataca
    20221 ttagaaaaga taacagaaat agttgaatta gaaaaagaat ctataaaaca aaataagctt
    20281 ttaagagatg aagaggatgg tagttataga aaaagggtaa taaatatgtt gttaaagact
    20341 ggtatgtttc cctttactaa aaataataaa gtggatgtat ttgtagatgg aaatgaaaag
    20401 tttaaaagac ttatagaaga tataagagaa gctaaagacc atattcattt agagtacttt
    20461 atcatcaaag atagtgaaat aggtagggtg ttaaaagaag aacttataaa aaaggctaaa
    20521 gaaggcatta agataagaat tttatatgat gatgtaggat gctggagatt ttggtttaat
    20581 agaaaatttt ttcgagaaat gagggaagta ggtatagaaa ttgcagcatt tttacctaca
    20641 aaatttccta taataggagg gaaattaaac tatagaaatc acagaaaaat tgttgttatt
    20701 gatggaataa ttggatatac aggtggaata aatataggag atgaatattt aggcaaaaat
    20761 gataagtttg gatattggag agatactcat attagaatta agggtatttc tgtatacatg
    20821 cttcaaatga catttctgat tgattggtat tatacgacaa aagaagtatt agtgactaaa
    20881 aattatttcc caagtgtagg gaatgttggt gaaagtatga tacaagttgt tgcgagtggt
    20941 ccagacagtg actgggaaga tattcattat gcttattttt ctgcaatatg tcaggctaga
    21001 aaaaatgttt atatagaaac tccatatttt attccagatg aaagtctact aaaagctata
    21061 aagagtgctg ctcttagtgg agttgatgtg agaataatat ttcctaaaat tgcagaccac
    21121 aagatagtaa atatagcatc ttattcatat tttgaagaaa tattaagagc aggtggtaaa
    21181 gtttatttat ataataaagg atttatacat tctaaagtcg taataataga tgataaaatt
    21241 gcttctgcag gtacagctaa tatggattta agaagtttta tgcttaactt tgaggtaaat
    21301 gcatttatct atgatgaaga agttattagg gtaatgacag atgatttctt tgaggattta
    21361 agtcactgtg aagaacttaa tttggaggta tttaagaata gaaatatcat acaaaaaatc
    21421 aaagaatctg tagcaagatt attttcacca atattataaa aaattattaa aaaaggtatt
    21481 gaaaattatg tgtgattggt atattataat aattgttagc actctattag atagagtgat
    21541 aataaataaa aagtatatat ataaatctac atatataagt agtattaatt taaaaataac
    21601 taaaaacata ggcgaactag tctatactta atattttagg aggcgtgaat aatgaaaata
    21661 agaccattag ctgacagagt agtaattaaa aaagtagaag cagaagaaaa aactgcaagt
    21721 ggaatagttt taccaggagc agctaaagag caacctcaaa tagctgaagt tgtagaagtt
    21781 ggaccaggtg gaatagttga agggaaagaa ataaaaatgg aattgacagt aggagataag
    21841 gttatattcc aaaaatattc tggaacagaa gttaagatag aaggacaaga atacacaata
    21901 ctaagacaga gtgatgtatt agctgttatt gaataaatat agaataaatt tattaggagg
    21961 ggtttaaaat ggctaaagaa attaaatttt cagaggaaac aagaagagct ttagaagctg
    22021 gtgtaaataa attagcagat acagtaaaag taacattagg acctaaagga agaaatgtta
    22081 tattagataa aaaatttggt tctccactta taactaatga tggagtaact atagcaaaag
    22141 agatagaact agaagataga tttgaaaata tgggtgctca attagttaaa gaagtagcaa
    22201 ctaaaactaa tgatgtagct ggagatggta ctacaactgc tacagtttta gcacaagcta
    22261 taataagaga aggtttaaag aatgtaacag caggggctaa cccaatactt ttaagaaaag
    22321 gaatacaaaa agcagtaaca gtagcagtag aagaattaaa aaatcaatca agaatagtag
    22381 aaacacaaga ggctatatct caagttgctt ctatatctgc tggtgatgaa gaagttggaa
    22441 aattaatagc agaagctatg gaaatagtag gtaaagatgg agttataact gttgaagagt
    22501 ctcaaactat gaatactgaa ttagatgctg ttgaaggtat gcagtttgat agaggatttg
    22561 tttctgcata tatggttaca gatgtagata aaatggaagc agttttaaac gacccatata
    22621 tattaattac tgataaaaaa atatctaaca tacaagaatt attaccagtt cttgaacaaa
    22681 tagttcaaca aggtaaaaag ttattgataa tagctgaaga tgtagaaggt gaagcattat
    22741 ctacattagt agtaaataaa ttaagaggaa catttgatgt agtagcagtt aaagctccag
    22801 gctttggaga tagaagaaaa gaaatgcttc aagatatagc aatacttaca ggtgctcaag
    22861 taatatcaga agaattaggt tatgatttaa aagaagctga tttatctatg ctaggtagag
    22921 cttcatctgt taaggtaact aaagaaagta ctacaatagt agatggttct ggagataaaa
    22981 aagctataga agatagagta actcaaatta aacatcaagt agagcaaact acttcagatt
    23041 ttgatagaga gaaattaatg gaaagattag ctaaacttgc tggaggagta gctgttgtaa
    23101 aagttggagc tgctacagaa gtagagttaa aagaaagaaa gttaagaata gaagatgctc
    23161 ttaatgcaac tagagcagct gtagaagaag gaatagtagc tggtggagga actgcttttg
    23221 ttagtgttat accagcaata ggaacactaa ttgagagttt agaaggagaa gttaaactag
    23281 gtgctcaaat agttaaaaaa gcgttagaag agccattaag acaaatagct ataaatgctg
    23341 gtcttgaagg tgctgtaatt gtgcaaaatg ttgttaattc tgaagcagaa actggatttg
    23401 acgcattgaa tgaaaaatat gtaaatatga tagaagctgg tatagttgac ccaactaaag
    23461 ttagtagaag tgctttacaa aatgctgcat caatagcaag tactttctta actactgaat
    23521 ctgctgttgc tgaccttcct gaaaaagaag atgcaggaat gccaggaatg ggtggcggaa
    23581 tgcctggtat gatgtaattt aaaaaattta aaatataaat tttaatataa agaactattt
    23641 cgacttagat tttaaactct aagttgaaat agttcttttg tatatatcta atttttgtat
    23701 atctataaat tcaatacaca tatttagata atcagtatct agtttgtaat aatattttag
    23761 agtagtattg ttatttttta ggttatgatt ttagatatat attaattaag gtataattgt
    23821 tgatagagaa taaatcaaat ttaaaaggat gcgataatat ggacttaaga aaattgcttg
    23881 aagaagttaa aaatgaaaat attgatatag attttgcgtt agaaaagtta aaagatttac
    23941 cttatgaaga ccttggatat gcaaatatag accatcatag agaattaaga aatgggtatc
    24001 cggaagttat ttattgtgaa ggtaagagtg atgagcatat tttagggatt attgataaaa
    24061 tgaatcaaaa aagttctaac atattgggga caaggtgcag aaaagaaact tttctgaaaa
    24121 ttaaagaaat ctataatcat gctgagtacg aagaggcgtc taaaattctt aagattcaaa
    24181 atcatgatat agagaatata ggaaaaggta aaattgtgat tgctacaggg ggaacttctg
    24241 atataccagt agcagacgaa gcttatcata cagctaagtt tttaggtaat gatgttgata
    24301 gaatatatga tgttggagtg gcaggtatac ataggctttt aaataaaaga cataaaatag
    24361 atagtgctag agtaatagta gccgttgcag gtatggaagg tgctcttgca agtgtaattg
    24421 gtggactggt tgatgttcca gtaatagctg ttccaacttc tgttggatat ggagcaaatt
    24481 ttggaggatt agcagcattg ctagctatgt taaatagctg tgcatctgga atatctgttg
    24541 taaatattga taatggattt ggagcaggat atttagcatc aatgataaat aaactgtaaa
    24601 tacattgata taaattaaac aaaactatat aataaaattt aatttctatt atatttttac
    24661 tattaaaatt gaggagacat atatgagtga aaaaatactt tattttgata taataagcgg
    24721 tatatcaggg gatatgaccc ttgcaagttt attaaattta ggtgtgccta aggaaatttt
    24781 tttggaggaa attcataaac taagaatgag cgatgaattt aatataaaca tcagctcgaa
    24841 aactgaaaat ggaatagtag gtacaaaagt agaagtaatt acaaaagaaa aacatacaca
    24901 tagaaattta gtggatatat ttgaggttat agataaaagc actctaaacg aaaatgtaaa
    24961 atctaaagca aaaaaaatat ttatggtaat tggagaagca gaagcaaaag ttcatggaac
    25021 tacaatagac aagatacatt ttcatgaggt aggggctata gattcaattg tagatatagt
    25081 aggagcttct atattagttg acttactttg tgtagataaa gtttgtgcta gtacagtgtc
    25141 tgttggttca ggatttgtaa aatgtgaaca tggaattata cctatacctg cacctgcaac
    25201 tatagaaatt ttaaaaggtg tgccaattaa attaaataat gtaaatggag aatgtacaac
    25261 tccaacagga gctgcaatca taaaagtttt gtgtgatgag tttgtagatg agcttgaatt
    25321 ttctccaaag caaattggat atggtatagg tcataagaag tttgaagtac caaatatact
    25381 cagaactgta ttaggtgaaa aaaaaaagaa gaattaattt atgaaattag tgcaaacatt
    25441 gatgatatgt cttcagaaat ctattcttat ttgtatgaaa aaatattaga tgagaaagca
    25501 ttggatattt atacagagag catttatatg aagaagaata gaccagcaac aaaagtatca
    25561 attttatgta gtgaatcaga tttagaaaaa tttattaaaa tattactttt agaaactagt
    25621 acttttggag tgagatataa tgcatataat agagaaattc tagagagaaa atttgtaaaa
    25681 ttagatacaa aatatggagt ggttacagta aaactagggt attataaagg aaaactagtc
    25741 aaggctactc cagaatataa tgaatgtaaa actatagcaa aaaatgaaga tattcctata
    25801 aaaaaagtgt ttgctgatat aaattttaga ataaaagaag aatttagtga aaacttattg
    25861 acataagtaa taaattttga taaattaata ttcagcctta ttaaataaaa gtaaacagaa
    25921 aaaaatatat aaaatataaa ctcactcgta tatgctcaga aatatggtct gagagtctct
    25981 accaagatac cgtaaatatc ttgactatga gtgaaattat tataccaaaa gacctagtta
    26041 agtaaatgtg taggctgagt taaactccct atgtgttggc tataagataa aggtattttg
    26101 gaagcctgta tagtaatttc acttttgcta gaaattatgg aagttactat acaggttttt
    26161 tgtgtaaaaa gatttaatat aatttagact gtattgtgtt aaaataaata tataaaaact
    26221 atattataca aggagagatg tgaatttatg aaacatgaat tagtacttgt aatagatttt
    26281 ggaggacaat ataatcagtt gatagctaga agagttagag aaaacaacgt atattgtgaa
    26341 atacttccat gtactgcaag catagaaaga ataaaagaaa aaaatcctaa agggataata
    26401 tttacaggcg gtccaaatag tgcgtattta gaagattctc caacaatatc aaaagaaata
    26461 tttgaattag gagtaccaat acttggaata tgctatggta ttcaaataat gtctcatgta
    26521 ttgggtggag ttgtaagaaa aggtaataaa caagaaaaag aatatggaaa aacagctata
    26581 acttatggaa aatcatcatt atttgaagga ataactacaa atagtgtatg gatgagtcat
    26641 acagatttaa tagaaaaagt tccagaagga tttactatag ttgcaaatac taatgattgt
    26701 ccagtagcag ctatggaaaa tgtagaacga aacctatatg gagttcaatt ccatccagag
    26761 gtggaacact gtttggaagg ggataagata cttacaaact tcttatataa tatttgtaaa
    26821 gtaaaaggtg actggactac agattcattc atagaagata aaataaaaga attaaaagaa
    26881 aaaataggcg ataaaaaagc tttatgtgct ttaagtggag gagttgattc ttctgttgct
    26941 gcagttctta ttcataaagc aattggggac aacttaactt gtatatttgt tgaccatggt
    27001 ttacttagaa aaaatgaagg aaatgatgta gaaagaatat ttagagagaa atttgatata
    27061 aatttaataa gagttaatgc agaagataga ttcttatcta aattaaaagg agtttctgaa
    27121 ccagaagcta agagaaaaat aataggtgaa gaatttataa gagtatttga agaagaatca
    27181 aataaattag gaaaaatgga tttcttagta caaggaacaa tctatccaga tgttatagaa
    27241 agtggacatg gaaatgctgc tactataaag tctcatcata atgttggtgg aatcccagaa
    27301 gatgtagact tccaagaaat tgtagaacca ttaagagagt tatttaagga tgaagttaga
    27361 aaaataggat tagagcttgg tatagaagaa ggattaatat ttagacatcc tttcccagga
    27421 ccaggtctag gaataagagt tataggtgat gtaactaaag aaaaatgtga tatattaaga
    27481 gaagctgatg ctgtttatat ggacgagctt agaaaagcag gtttatacag agaaatatgg
    27541 caagcttttg caacattacc agatgttaag actgttggag ttatgggtga tgaaagaaca
    27601 tatgcttatt tagttggttt aagagcagtt acttcttctg atggaatgac ttctgactgg
    27661 tataagatgc cttatgatgt attagaaaga atttctaata gaataataaa tgaagttgat
    27721 ggagtaaata gagtagttta tgatataact tctaaacctc ctggaactat tgaatgggaa
    27781 tagtaaataa aaaagtcgta agccttgtat tttcaaggtt tgcgactttt aaaaaaatct
    27841 ttgtgatact aaaatgatac tatatattta aattatctag tttttgggca acttcaagtt
    27901 gcttatttgg gtataaatgt gaatatgtat tccaagttgt ttctactttt tcatgcccca
    27961 atctttctgc tatagttaat atatttacat ccatatttac caataatgac gcatgagaat
    28021 gtctcaaatc atgcactcta attcttttta cattagataa cttacagcat ctatctagct
    28081 ctttagaaag ataactttta gcaaatttaa atattctttc attatctttt aagtcatata
    28141 gcttagatag ataatctttt atattattat ataagaaatg tgggataggg acaacacgtt
    28201 tacttttagg agttttagga gaagaaacaa tatcctctcc atttagtctt atgtagcttt
    28261 tttcgacgct tattttattt tcgaaaatat cttttggagt aagagctaac aactccccta
    28321 gtctaagacc tgtccaaaat aaaatttcaa atgctagtct aggttctgat ttcttttcaa
    28381 attcaataaa ttttttaaac tcttctaatg tccaaaagtt catttcatct gcgttttttt
    28441 taccgataga acctgccaaa tgagcaggat tagaaggtaa gttataatat ttaactgcat
    28501 aatttaatac agcaacaagc tggttgttta tcgttttaat atatgtttgg ctataatcac
    28561 tttttaataa ttcgttttgc catcttctta tatgtgttgc agtaatttca tttattttta
    28621 gacttttaaa aaaaggtaaa attctaaagt ttattaaata ctttttagtt tctaatgtag
    28681 ataattttaa tctagaagac atatcgtgca tgtattcttc tatgagactt tcaaaactca
    28741 tatcagtact cattttagat ttattcaaaa attctctttc aaactctaga gcttcttttt
    28801 tagttttaaa acctctctta attttctttt tcctatcccc atcgaagtct gtaaaataaa
    28861 agctagcata ccaacttttt ctttgttcgt ctttgtaagc aggcatttta gacaccccca
    28921 attatgtcat tttaatatta caataattat aacatgttag atatgctatc taaataattg
    28981 ttttattttc ctcttctttt tatttcttga agcttgtaat atttacacag ttctaagaag
    29041 tcttcttcta gtgctataga taagtctaat atcgtagata tacctatatc tttgtactct
    29101 tgattttcta gttttgatat ataacttctg ttacgtctca ttttttctgc taattctagt
    29161 tgtgtcattc ttttcttttt tcttaatttt ttcaacattt attaaaatcc accttttaat
    29221 tatttttatg acatttttat aattaatagt ttgtgtaatt tcttgtaaaa aatgttcctc
    29281 attagaacat tttttggtgg aaaaatgtgt taaaatgtaa gtaagaaaag cactttcaaa
    29341 gcactataaa aatagtgctg aaatagataa acaaataaaa gtaataagga ggaaagatag
    29401 attctagagg gaaatcatta tcatttttaa gaacgtatgt tcggttggtg ggatagaaaa
    29461 atactttaca ggggatggtt tatttgaatg agaatgaaaa tatgagttta tatgtcagta
    29521 agttaaaaga agtattgaaa aataattcag aagagtataa aaaaataaga gctaaaataa
    29581 atgaaattta taatctaaat gaaaagaaag aataaaaggg ctttagctct tttattcttt
    29641 cttagtatta actgctttat aataagcatt atctatcatt ttttttattg catttttatc
    29701 atctgaatct aaagatgcta atctatttat tagttcgtta acttcattat cttcttttga
    29761 gttaactttt tttaataatt catcatcttg ttgagtgtta ggttgtattt tacctaattt
    29821 agttaatagc tcctttaaag ttaaatttaa tccaaaactc attttttcaa gcatatctaa
    29881 agtaggttca acagctttac cattccttgg gtctctgttt ttttctatct tatctatata
    29941 tgtatggctc acaccacaaa gattagaaaa ttctctaaga gataagttat gttcacttct
    30001 atattcttta ataatttctg ctaatgtttg cataatttca cacctcatct aagtaatagt
    30061 tgacttaatt atatcagaaa aatgtaaact attgttaaca aaaataaaaa tttagtaaaa
    30121 aataattgac aagagtaaaa aaaagacgta tactatgatt aacaaaaagg ttataaataa
    30181 agttaggagg gattaatata aaaaataatt tacaatatat tagaaaagaa tctagaatat
    30241 cacaaaaaaa attcgctgat aagataggta tatcaagacc gtatttatct agaatagaaa
    30301 acggtaaggt taatccgagt ttagaaatag cgcataaaat ctcgctcgaa acagggaaaa
    30361 caataaatga aatttttttt gattttactg taaatcatag taaactataa ggttttaatt
    30421 gtaccttgaa aactaaatat aaaatatttt aaaggagcaa gtatatgaaa aataaagaaa
    30481 aagaaataat cattagagga attttaatag gaatttttta ttttttagga attgtattta
    30541 gtaatacttt ttttaaataa taaatagaag gggtaggtga aataccaact ccccaaatca
    30601 aaataacact ttggaagtta aatataaaat attcaaaaga ggtgattaga tgcaaagaga
    30661 gcaaacaacg cttcgtatac cagaagactt gcacaaagcg ttgatagatt taagcagtga
    30721 tataggaatg cctattacat ctattataat aattgcatgt tggttgtata tatcaaagat
    30781 aaattaacca atccgatatg caaaatgaat gcacaacatt atttattaga gaattgattg
    30841 ataaattatt ttctttagct gttttctcta gcaactcttt taggacacca ggaatcctaa
    30901 gagtagaacg aacagtatca tcacttttat acgagattga ttgaagttca ttaacttttg
    30961 aatttctaat aatatcatta atagcgtata gaataagcga agatttgaga atgcctgttt
    31021 gataagaaat tttttctaaa ttttcattta atggtgtata tattcttaca gtaatagtag
    31081 tcattttgac acctccctaa tgacattata caaaaaatat ttaaaaaagt cttgacacta
    31141 aaatagtgtc ataatataat gattatagac agtaaaacag tgtcataagg agggaggaat
    31201 aagaatggaa aaagtaagaa tgacagtaag gctcgtccca aaattaaatc aatatgtaca
    31261 gaatatagcc aaagaatgtg gaaaaagtaa aaattctatt attgtggatg catgttggga
    31321 gtttattgag aaaataaaaa aagaaaataa attcaatgta gaaagcgagg aataaatatg
    31381 aataatctac agatatttga aaaattggaa tttggacaaa taagaatggc agagattgat
    31441 aataagcctt actttgtagg aaaagatgtt gcaaagtcat taggttataa aaatactaat
    31501 gatgcaattt taagacattg caaaggggta gtgaaacacg aggggtttaa aattaatggt
    31561 attaagattg ctttgataac tgagggtgat gtttatagat taatagtagg ttcaaatcta
    31621 ccaaatgcag aaaagtttga gagttgggtg tttgatgaag tgcttccaag cataagagaa
    31681 acaggtcaat accaagcaca acagcatgta ataacagaac ttacaggaac tataggagac
    31741 ttaaaaggaa caataaaaga atataaaaag ttgtgtaaga taacatgctc taagaaacaa
    31801 cagtattcta aatacataaa gaatagactg gatatagaca aagctaataa agaatacaac
    31861 caagtgaaag caaggctatt cctaattctt ggagttgaga agtgggaaga catagacttc
    31921 gatacatcta ataatttaat acaaattata gatgattgta taagagttat aaagtcagag
    31981 agaccataca agcagttgag ttattttgaa aactaaatac agaatatttt gaaatatatt
    32041 gttttaatta attattaact aggaggttaa atacatgaaa aataatataa gcgattcaag
    32101 agtaaaatat ttttgcaagt gtccatactg tgggtttgat aatgaggtag aagttaaaaa
    32161 aggattgaag cctaaaatat gttgcatatg tacaaaagaa gttgagtatg aaaaactgga
    32221 gcaacaaagt actccagcaa aataaaatta ataaatatga atatttttac aagaatctaa
    32281 ttctttttct aacttattaa cagcatggtt atattgcagt gcctgttctg ctataaagtt
    32341 agaatttgat atgtgttctt tacacatagg gcatggtagc ccattatcac aaacttccct
    32401 aaatttagat tctgttatgg taacttttgt accacaatcg aaacaagtag cttcaatttt
    32461 agttatttta ccgatattca taaatcacca cctaatcata tatttatagg atttatccta
    32521 caaatatagt atatcaaagg agggaaataa tggcaattaa tgacaacata aataaaattt
    32581 taagagatag aaatttaaaa gcgtggaaat tagcaaaaga aataggcgta gattcaggaa
    32641 atttatatgc aattttaaga ggagaaaata aaaatccaac tatagataca ttgataaaac
    32701 tagctgacta tttagatgtt acattagacg aattagttgg aagatagaaa ttaaatacag
    32761 aatattttga aagtaggtgt attgtatggc aaaagcagta gctaaagaac aattgtttta
    32821 tagggcaaaa gatatagcta agattttaga tgtatgtgaa gcaaccgctt ataaaataat
    32881 tggagaatta aataaagaat tagaaaaaga aggttggaag acctttcctg gaagggtatc
    32941 ggtaacttat ttcaaagaaa gatattgtta taaaacaaaa aaaggggcat aaaaaatgct
    33001 atgagagtag ctaaagatat gtttcctaaa gtaattgtat aaagaggaag ggggtgattt
    33061 agttgaatgt aagagtactg atagcttata tacagttttg taagcaatac aataagaaag
    33121 cgagttttga aggtcttaaa aaatacaaca aaggggtaat tgcatgagaa taatttataa
    33181 aaacaaaatc tacaaagtag aacaagacaa agtgttattt agaattacat actatgatga
    33241 gcagaaaaat aacaggaagt ttaataacaa taagaaagta aaaagaagtg ttttaacaag
    33301 agatatagag ttagttaact tgtatttacc agcacattta aaaataaagt aggaggttta
    33361 attgcatatg gtatttaatt tagagaagtt caaagtggga aatgcgataa gaataagttg
    33421 tgaaagattt ggttttgaaa ttgattgtat tgtagtagta gcaactgagg aggaactaaa
    33481 tttagcttac tttgataaag aaagaggttg catagagtat caagcattga tgccagaaga
    33541 ccttagatat gatgattata ttctcgaaag attaggttag agggggtgta ttttaatgga
    33601 aacagctata tctattgcaa aaggtcaaat agaaggagcg aatgagagta taaaggaatt
    33661 aaaatcaaaa gaaaattacg ataaagaaag tttaagatgg tgggaaggtg ttaagcaagc
    33721 tagtgaaaat atactagagt ttttagaaat agaaaataag gcataagaaa agagccatta
    33781 cgattggctc aattcaaaaa cataataaaa tttaattagc tatattatag cacaaggggg
    33841 aactaaaatg aaagcaattt tattaaaaag tttaaatata gagaatttca aagggataaa
    33901 agaacttcgc atagatttta acaacataac taatgtgttt ggagaaaatg caacaggtaa
    33961 aacaagtata tttgatgcct ttacgtgggt aatgtttgac aaggatagca agaatagaag
    34021 cgtatttgag ataaagcctt tagacaagca aaataaagtc attagaggac ttgtaacaac
    34081 tgtaacagca gtgctggaag ttaataataa agagataaag ttaacaaaga aatatgaaga
    34141 aaaatggact agaaaaagag gagaatcaga agcaactttt actaaaaacg aaacaacata
    34201 catgataaat gatactccaa taaaaaagag tgaatatgta aaagaggtag ctgaaatagc
    34261 aaacgaggaa cagtttaaat tacttactaa tccgtatttc ttttcgaatg agcttaactg
    34321 gaaaaaaggt agagaattaa ttctaaatgt gtgtggagat ataacgacag aacaaattat
    34381 agaatcaaat aaagatttaa ctttacttat tacagagttt gaaaaggaaa ataacataga
    34441 taagattatt aaaaatagaa aagctattaa aaataactta tctaaagaaa aagaagaaat
    34501 accagttcgt gtaaatgagt gcaataaaag catgtatgac atagattttg aggaaataga
    34561 ggctcaatta aaagctaaaa aggctgactt agaagctatt gaggacatgt tactaagtgg
    34621 cacaaaggcg aatgaacaga ttttaaaaga taaagaaaag atatttgagc taaaacaaga
    34681 gacacaaagt attaaacaat cagcaagtga aaaaggaaat aagaaaagga atgagctgtt
    34741 aaaagaaaag gatgagttga aatataatat taaaaactta agaaataatc ttgtttattt
    34801 agaaagggat aatgagttaa aagaaacttt gaaaaaaaga gcgattgaaa aaacaacaaa
    34861 tttaagagat aaatggacta aaaagagtca ggaaacatta gatttaagtg ttatccagac
    34921 agaatgccca acttgtaaaa gacctcttga tttagaagat atagaagaaa agaaaaagga
    34981 aatgttagat aattttaatc taaataaagc taaagaatta aaagaaatag cagagctagg
    35041 gaaatctaaa aatgatgatg tagaattatt taatacagaa attgaaagat taaaagtaga
    35101 tataaataag actttagaag agatacaaga aaaagcagta ttattagata aaataaaaaa
    35161 agaattagaa agtacaaaat ctacagagat atattcagca gatgaggaaa aaagattaac
    35221 agaaattagt gcagaaataa aagagttaga agaaaaaata aacaataaag atgctgataa
    35281 aaatatagat gagctaaaag aaaataagaa aaaactgact agtgaaattg agttacttaa
    35341 taaagaacta gctaaaagag atattaacaa agagttattg gatagaaaag agcagttgtt
    35401 agaaaaagaa aaagcgttag gtatagaact agctcatcaa gagaaaatac tcaacttatg
    35461 tgaattattt ataaaaacta aagttagttt attagaaagt aatataagta gtaaatttaa
    35521 gaatgtaaca tttaaactat ttaaagagca gataaatggt ggtatcgaag aaacttgtga
    35581 agctttaata aacggtgtac ctttcagcaa tgccaacaca gcggggcaaa taaacggagg
    35641 gctggatata ataaatactt tatctaatca ctttgaagtt aaaatgccaa tattcgtcga
    35701 caacagggag agtgtgaata atttgattga tattgatagc caagtgataa atttgattgt
    35761 aagtaatgat aacccattaa aaatagaagg ggtgaactaa atggcagaaa gaaatataac
    35821 tgatgtagtt ttaaatagag tgaatgagct aaaagatagt ggtgagttag taattccacc
    35881 cacttattca gcagtaaatg ctttaaaatc tgcatactta attttacaag aaacacttga
    35941 taagagctat aagcctgtac ttgaaacatg tactcctatt agtataatca attctctttt
    36001 gaacatggtc atacaaggat taagcccagc taaaaaacaa tgttactttg ttgcttatgg
    36061 gaacaaattg caacttatga gaagttatat gggtactata gcagttacta aaagactttc
    36121 taatataaaa gatataaaag cgtattgcat ctatgagggt gataaattca aagtcaaata
    36181 caataaagaa aatgctgtac tagaaatagg agaatatgaa ccagcttttg aaaacataga
    36241 tataggcaaa ataaaaggtg cttttgctgt aattgttggt aatgaaggaa tcttgcatac
    36301 cgaaataatg acctttacac aaattgaaag agcatggtta caaggtgttg gctatagtaa
    36361 tcccaaaaat aaagtacatg ataattttac agaagaaatg gcaaagaaaa cagtaataaa
    36421 tagagcgtgc aagatgtatg caaataccag tgatgatagt gatttattaa taggtgcttt
    36481 taatgaaact gatagagtaa tcactagcga agaaattgct aaagatacag acaaagaagt
    36541 agaaaaagag atttctcaaa atgctaatat caagtcttta gaaatgcctt cacaagtgga
    36601 taatacaatc tttactcaaa ctaaagaaaa agtagctatt aacatagatg aagataagcc
    36661 gaatctagct gaatttgaag aagcaatcca agttgaattg gatacaccac cattttaaat
    36721 atgattaaga tattagggag cagtagcaga ggtaattgct acttgctcca actggaaaac
    36781 gagacattaa ttttagagtg tggagtcagt tacaaagaga tattaaaagg cttagatttc
    36841 agtttagaaa atgttgtagg atgtttggtc acacatgaac ataaagacca ctctaaagct
    36901 atcacagagc ttacaaataa tggaatagat gtatattcta gcaagggtac attagaagct
    36961 ttaaaaatag aaaatcatag aactaagatt atagaaagcg agaaattatt caagataggt
    37021 aactttaaga tagtgccatt taatactaag catgatgcgg tagagccatt aggattttta
    37081 ataaatcata gtagttttgg taacctatta tttataactg atagttatta ttgtgaatac
    37141 aactttaata atttaaatca catcatgatt gagtgtaact atagtaaaga tttattagat
    37201 aacaacaaag ataagattta tttgagaaat agaatagtca aaagtcattt tgaattaagt
    37261 aatgtgatta attttctaaa agtaaatgat ttaagtaata taaaaacaat tacattgctt
    37321 catctaagtg aagataacag tgataaagat ttatttataa gagagataga aaagaatgtg
    37381 ggagtaccag taatagtagc tgaaaaagga ttagaaattt atttagacta gaggtgataa
    37441 agtggcaaaa tatagaattt tacaagctaa cttttgggat gatggatttg tattagattt
    37501 aactcctgaa gaaaagtact tttataacta tcttttaact aatggtagag caagtcaatg
    37561 tgggtgttat gaattaccct ataaaattat ggaaatgcaa acaggatata acagggaaac
    37621 tgtagagaag ttaattaaaa ggtttataga gtatggaaaa ataaaatatg actctactac
    37681 taaagagata ttaataatta attggagtaa acataatttt tctaaaagtc caaaagtacg
    37741 tacttgtatc ttaaaagagg ttgaatcaat taaaaataaa gaatttcagc agtatatgta
    37801 tagagtatgt atagactatg gataccctat cgatacagta tctatagact atggggaaaa
    37861 agaaaaagaa aaacaaaaag aaaaagaaaa acaaaaagaa aaagaaaaac aaaaagaaaa
    37921 agtagaaccg ttaatttgtg aagaacagga aaaaaatgag ttaaaagaat ttaagaagct
    37981 atatgaagaa aatataggag tggtatatcc agttacagca gaatggttaa tagaaatttc
    38041 tagtgatgta gatataagat tatttaaatc tgctatagag atatgtgctg aaaagatgaa
    38101 tatgaatata gcatacttaa aaggtatcct taaaaagtgg aaggatgcga atataactac
    38161 atatgaacaa ctagaatcat atagattaca gcaagaaaat aagaaagcaa aaaaagtagt
    38221 taatggccaa ctaagtaaaa ataagtttgc caactttgaa caaacattta ctaagtatac
    38281 agagaatgag cttgataaca taattaagaa aagtcaagat gctaagttca aataaaatta
    38341 aacttctagg aagtaaatat caatatattg cttcctagaa agggggtaga atatgggtaa
    38401 aatttggatg gatgcaggaa catttttgga aactaaagat ataggagata ttgaatgtaa
    38461 tctaagagaa atgataaata aaaataaaca aaaaaattta attggtagaa taaattccaa
    38521 aggacaagga agaaaaagta agaaagtaga gtgtactaac atcattactg gagagagtaa
    38581 aatatttgtc agtgctgtag aagcaagtga atatttatat ttcacggaac ttcatattac
    38641 tcgtctagca agaacaggaa agactactaa aaatggttgg aaagttagat atattgaaga
    38701 ggtgtcaaat ggtattagca aatgtggaac aagtaattaa gttagctgaa aagatattaa
    38761 gtaagaaaaa gtgttctgtt aataaagcta ttgatatagc tataaaaata ttaaatagat
    38821 acgaatatga ggggatgatt aaatgagtct aattgaatat agaggttatg attttgaaaa
    38881 taaaaagtgg atttattcta aaacaataat gtggagtaat gcagtagact gtttattaat
    38941 gctgaaagaa gattttaaat ggcgaaaagt ttgtaatgtt ggaatatgtt ctgaagaatg
    39001 ggctggaaat aatcaagaaa tttttgaagg ggatatatta aaagaaaatt ataatcctca
    39061 caatagaagt tcatatggaa ttgtaaaaag agattttaat agtattaagt tatatttgga
    39121 gtggcattat ttaaagaagt ttgaaggaga atggatagag attatagata aaacagaaat
    39181 atatcatagt agaggttaca aagtaattgg taatgaatat gagaacttag aggaagttag
    39241 gaaagagttt ttagaacgca aggagatgtt ggaaaatgaa cgtcttagct agtttaatac
    39301 ttatagtagg aagttttata gctggtaggg tttatgagta tagattgaat ctaaaagagt
    39361 ttgaaaatta tgataatata ggaggtttta agaatgaatg atagattaga aatgataaat
    39421 gcttctgtaa attatataca gatgatatgt gaaagttcaa atatagctat tatagcagag
    39481 catgaccgag ttagaatatt ggatttagaa actaaagaaa agtatgacct attaagaagt
    39541 gagggggatt aaaaaatgag taaagttata caatgtgatt tctgtaagga aatatttgaa
    39601 aaatataatg gagaatgtat tgagctatat aaaaaggatt gtagcaaagg ggtgcttggg
    39661 atagataaac acatatgccc aacttgttat gaaaaattca ttgaaggaaa aatagagaaa
    39721 gtagaaaatg ctaaaagatt taaggataaa ttaatagact tcttggttga gcatgaggtt
    39781 tgtgactgtt gtgcttgcga ctgtgtgttt gattctaatg acgaaaaaaa atgcaaggca
    39841 ggaattagag agtgggttga aagtgaggta gaagaataat atggcaaaat taatagttag
    39901 cataccttta actaaatatt taaaagatat aactaaagaa tggggagaaa cttatataac
    39961 taaaacatat ggtgggcatg tctatttaag tgatgaagct ccaggagaag tatttaaaaa
    40021 agaagatgat ttaaattata tagagtttaa cgattctgat attgtcatga gattacctaa
    40081 agaagtacat caacatataa atttgaaaaa aggttgcaac aagaatctta aagatgtatt
    40141 aaaagaaata aaaaagatag aagagaaaac aaatatatag aaaatatcta attaaaacag
    40201 tttagagagt tgcaaaatat cttttagtat aaatttattg ttgaagtgtt ttgtgactct
    40261 caaaaatgaa aataaggagg agctatattg cttacatttt tagatttatt cgcagggata
    40321 ggtggtttta ggctaggaat ggaaaaagca ggacataaat gtttgggaca ttgcgaatat
    40381 gataaattcg caaatttaag ttataatgcc atgcacaaac cgaaggagga tgaatggttt
    40441 gaaagagata ttagagaaat tagaacagaa aatatcccaa gagcagatgt ctggtgtttt
    40501 ggattcccat gtcaagacat ttctgttgca ggtaaacaat ttggatttaa aggaagacgt
    40561 tcaagtttat ttttcgcagt tacaaaactt attagagaac tcaaagaaga agatagaccc
    40621 aagtatttac ttattgaaaa cgttaaaaat ctacttagtg ttaatggagg atttgatttc
    40681 ctcaaagttc tcattgaact ggatgaaatc ggctatgatg cagagtggca agttcttaac
    40741 tctaaaaact tcggagtacc ccaaaataga gaacgaatat tcgttgttgg acattttaga
    40801 ggacgaagta cacgaaaagt atttcctatc gaaagaaaaa gtagaaaaaa tcttgaacaa
    40861 ctaaataatc caactcatag tacaagtgga gttttagtca atgcagtttt aacacccgat
    40921 agggtaaaca aaagacaaaa tggtcgtaga attaaagaaa gcggagaacc aatgttcaca
    40981 ctaacagctc aagataaaca tggaattttg aaaaatggag atataagaag gttaacacca
    41041 aaggaatgct ttaggttgca aggatttccg gataaatatt acgaaagagc agcaagtgta
    41101 tgctcagata gtcaactgta caaacaagcg ggaaatgctg ttactgcaaa tgttgtatat
    41161 gaaatagcaa aaagaatggg ctaagagttg caaaatgtct tttagtatga gtattgttga
    41221 agtgttttgt aactctcaaa aatgaaaata aggggtgaat aaatgtcaaa gtatgtgctt
    41281 agatggcaaa aagggttatt gttggatgaa cgtaagataa attattcatg tggaagtaaa
    41341 gaaatgctaa aacaaaaagc agaattatta gctaaagatg acaagatatt gcttgtaaca
    41401 atagataaag ttgaagaagt tataaaagat actagaagtc aaaaaatggc tgaatattat
    41461 tgtgatggag gaattgaaat atgataatac acaaatttat aatacatgtt ttagataaga
    41521 atagcgatac accaatacta aatgattttg agggtagggt tagtcaagat attgaagctt
    41581 tctttcaaaa gaaaataagc aaagtatcaa gagataatga catcagaaca gtagtattta
    41641 atgactatag taacaatcta attaagaagt gttgtgaaca aattatttat gatgaaagtt
    41701 catttttaaa taactctaaa gagattgcag cttatttatt tgatgttatg aaattgaatg
    41761 ctatattaga atcttgcgac ttagcaattt gcttatactc tcaaaaagat gaaaagaaag
    41821 ttgctatatt aaagcttgat tacaataatt cgtatactca ttctattagc tttgaagagg
    41881 ataaatttaa tatacagatg tctaagaatg aaattaatat acaagagact aagacggtta
    41941 aaattgctgc tttggttgga ttgagtggaa tgaatgacga atatcatcta aaagtattgg
    42001 ataaggatgc agagaaggaa gaagctaatt ctaagtttgt tacagagttt ctaaatgcta
    42061 ctaaagtgaa agatgacaag tataggacta aaaagtttaa agatacagtt gaaaattgga
    42121 taactaatgt tcttggaaat gatataaaac aagctgagga cataagaagt atattaaatt
    42181 atactttgaa agaaaagcat gaaattgata taaaagattt tgttgataaa tcaattaaag
    42241 atgatgagtt aaaaaatagt tttaaagaac atatggaaga aaagggtctt gatgaaagtt
    42301 ttagcataga taaaaaatgg gttgaaaaaa agcttaaaaa gagaaatata aaaactgaca
    42361 atggttttga aataaaaggt aacttaactg attttgagga cccaatgaaa tatacagtaa
    42421 gacaaaatca aaatgggtct atagatataa ttattaagaa tgttaatttc tataatgaaa
    42481 aataaggggg ctacaatgga aaataaagat aattttgtat taaataagct tggaccagca
    42541 agtaaagtaa tgaaaaaagt tgaaatagat gaaaaagaaa ttaagagtta tctaaaagaa
    42601 ctaaaagaat gtgaagaagt aaaagttttt attattagaa aagatggaat tgaaattcca
    42661 atagttttca aagatggaaa tatgaacgaa tggttaagaa aagaaattga gataagtttt
    42721 gatagattta aggatgaaat tgctcaagtt aaaaaagcat tgattaaagt tacaggagag
    42781 aattaagaag ttagaaggtg ctcatatgac taataaagaa atgtgcaagt caaataatct
    42841 tgatgaaaga gaagtgtgta aggaatttgg aaaagagatt tgtggtagtt gtataaatga
    42901 tagggtagat tgcgaaagca aagattgtga tatagcatat gaaaattggc tagagaagaa
    42961 tacagaaaga taattataaa aataaagtca aggtaagttt gtgaatgaaa ctagaatgtt
    43021 atagacttac tttgacttat aaaaaaggag tgtattaaat ggctaatata tattgcgaaa
    43081 attataattg taaaaactac tttgaagata tgtgtatgct tgaaagaatt gaaattaata
    43141 acctgaaagt gtgtgaaagt tacattgaag gtaaaaatga gctatataaa ttagaaaacg
    43201 gatatactat acatcctaaa gatttgaaaa tggtgaaaag taaagattat tctgttgaag
    43261 ttactcatat tccaactggt attacagtaa aatgccgttc tacaaatagt attttaaaaa
    43321 ataaaaataa gtgtttggaa gttctagaag aagaattaat aaaaataaac tctcacttag
    43381 agctagaaga tttaaactaa ataggaagtg agcttatgaa acgaagaaga tgcagttggt
    43441 gtggtaagct attttatctt aaggaaaaat ctaaggatgt ttattgttgt aaagaatgta
    43501 ggaagaaggc taagaaggtg aaaaaatgaa agtttttctt gtaatagatg gagagccagt
    43561 tggcaaggaa agaccaagaa tgaatactta cgctaaaagg acctatacac ctaataagac
    43621 taagaattat gaggacctaa taaaatggct atatcaatct aaagttaaat attactttga
    43681 aggttatata aaaatgactt taagatgtta ttattctata gctaaaagta acagtaaaaa
    43741 ggttaaggag cagaaaagaa ataatgtgtt aagacctagt aaaaagcctg acattgataa
    43801 tgtcattaaa gtagtagctg attcactcaa tgagatagct tataaggatg atacacagat
    43861 tgttgaggtt gtagctagta aatattacag tgataaacca agagttgagg ttatattaga
    43921 agatgttatt taaccaacgg aaaaatccgt tcgttagatt aggtactcag tttttcataa
    43981 aaatgtgaaa aagctgaata gagaatatat caaatggtaa aggagagata aattatgaat
    44041 gaaaatataa ataaagaaat aacagtactt ggaactttag aaatcgaggg aatgaaattt
    44101 cataacattg agggtggatt tggagaacat aagaaagcaa tgctagtaaa agatatagct
    44161 gagatacata atagagaact taaacatata aatgagctaa ttaataataa tataaagaga
    44221 tttaaaacaa atatagatat tatagattta aaagtgagtc gttctgagcg actaggtgat
    44281 ttaaaggcta atccttttga aggattaggt tacgaggatg ttggatatag taaacaatct
    44341 tttaatcagt caagaaatat ttacttgtta tctgaaagag gttattcaaa actactaaag
    44401 atattagaag atgataaggc ttgggaacaa tacgagaaaa tagttgatgg atatttctct
    44461 atgagaaaag agttaaataa ccctctttta agtgcatcga aggagttaca ggctatattt
    44521 atgctagata agaaacaaga agtcttagaa actaaaatag agaatgttaa tgagaagtta
    44581 gagaacttta tggatgatgc accattattc aatatcgagt gtgagtgtat tgttaaagag
    44641 gttaagagag tagcaacaaa atcacttggt ggacatggaa gtaaggctta taaaaataaa
    44701 tctttaagag gtaaagttta tagtgatata taccatcaga ttaaacgaga atttggagta
    44761 gatagttata aggctataaa gcgttgtcag ttagataaag tattagagat tgtaaataat
    44821 tataagttac ctatagtgtt tgaagaagaa ataagacttt tgaatagtca attatcaatt
    44881 gtgagttaag acaggaatag atttttctcc tttaaagggg aaaacctgca atagttaatt
    44941 aaaagagaaa aggagtgctt tcacactcca cttgtcaaaa atataaaact tttatataca
    45001 agattattat aacataaata attgatagga gtgtgtgagt atgtctaaaa ctaaaaaaga
    45061 gttttttaat gcaactaaga aacaactttc taattataaa caattaagtg caaatataat
    45121 aaaattaaaa aatgaaatac aaatgttgaa agataattcg gttggagatt taatgaaagg
    45181 tataagttat gatagtgtca aaacaggaaa aacaaacaaa actagtaaca tgattgagga
    45241 tgctattgtt aatgtatcag acttaataac agaaaaggaa atagagttat atgaagcaga
    45301 aataattaaa tccacaatag atttagctat aagaaactta aaacctatac acagacaaat
    45361 tattgaactt aaatatatag atggtctaat gtggcaagaa atggttgata tagtacattt
    45421 agaagaaaga caattaagtg taagagctag tcaagctatt agctcaatat caatagcatt
    45481 atttgggaag aaagcattaa tagagcaaga accacttttt gaattgttag attacaaact
    45541 aaattaaaaa agtaagagta attttgagtg ctgaaaatgt gcaggttttt ttgttttaga
    45601 catgagataa tagtattgtg gaaatgaata tttctctctc aaaactaaat atatatgtgg
    45661 gctaggtaaa gggattcacc tagcttatat gaacagacta ggcagggcgt gaggacgctg
    45721 ttagttcaat tctaactatg ttcaatcttt agtttttcta tttcaattaa tctacggata
    45781 cactaaaaaa tagtatttga attgagatta aaatctcata cagttttgta tcttaattca
    45841 gagtctaaac cgagtggggc ttggtaacct cactcaccat gcaggtactg gtgtttaatc
    45901 taagttcgat tcttagaact tgcgacataa tatatgtatc tccctattta aaaaggctaa
    45961 gtgagggtaa gcttagtctt ttaatttaaa aaaaaaagaa atttttattg tcataatact
    46021 atttgcttag gtatattata atgtgcatac ttaaaattaa atacttagca agcatttgaa
    46081 ttaatataca taaaatatat gacataattt tttatagtgt aagttattta aattaatata
    46141 aataacagta attttattat agaaaatgta tatattgtga ataataataa taataaaatc
    46201 ttgtacaaaa tgccaactga taattcctcg aaatgtattg tgtatttaac gttacgattg
    46261 tataattaac ttataatagt gatatggaag gtggtactta tggctacaaa aagtatttta
    46321 aaaaatgtag atgtaagaaa aaaggcattt ggaagaaatc tagtatcagc tctagaaaat
    46381 gctaaaaata aacaagaaaa agaagttgta ttaagtaaaa aatgttcaga agtaccaaaa
    46441 gataaaataa aggatatatt cgggagattt taatgagtgg ctatttaatt gtaaacttaa
    46501 gtaatatgct gggagaactg gaggaagaag aagttaaaaa aattctctcc agtttttctt
    46561 gtcctcttaa taaagatgta gaagactttt taaaaaacaa agctattgaa ttttctaaac
    46621 agggtttagc tagtacacat ttagtattaa cttcttataa aggcaagcct gttatagttg
    46681 gatatttcac tctagctaat aagtatttta caataaaaag aaaaacatta acaaactctt
    46741 tagctagaaa agtagtaaag tttggacaat ataatgaaga actaagaaga tatattattg
    46801 gagcaccttt gataggacaa ataggaaaaa attattcaaa taattataat aaattaatta
    46861 aaggtgatga acttctaaaa attgcatgtg acaagataaa agcagtgcag ttagatatgg
    46921 gtgggaaaat agtatatctt gaatgtgaag acaaacctaa gttaattgaa ttttataagg
    46981 ataatggatt tgtagatttt ggtaaaagaa gtcttgataa agatgaaaca gattcattgg
    47041 atggggacta tctagtccaa atgttaaaat atctaaaaaa ataaaagtac ataaaatcta
    47101 aaatgactat cttgatggat ggtctttttt tatacaataa attaaaagga gaattataga
    47161 atgaaaaaga taactaaact tatattatgt tctttgctag taatcagtat agcgagtcca
    47221 ttacaagttg aagctaaaac tactcatcat tccagttcgc atgtttctat tcataagagt
    47281 agtacaaaat ctaaaagtag tagacctaaa attactaaaa ctaaaaaagc tactaaaaca
    47341 tatagaacta acaaatctat caaaaagaaa ataaaactta aaaaacaagt aaacaaaaaa
    47401 actcaagttc caaataaaca agctgctaga aaagacaaaa aaacaactac atataattat
    47461 tacaatagct ataactcaaa tagatactat actacttcaa gtagcgggtt aagtttctgg
    47521 gattgctata tgttaggtca tttattcaat aacaacaata aagtttcaga gcaagatata
    47581 gctagagagt tgcaaaaaca aggttatagt aaacaggaaa ctgataatat attaagaaaa
    47641 gcaaatcaaa atcaagctaa aaaagaagaa aagaaaaaaa gagtgatgat gttaatattc
    47701 tctgtattag gagcattgtt cattataggt ttagggctaa taatatttat caaaagggaa
    47761 tcaaaaggtt gcaataaatc aatgtaattt aattagatac atagactcta acaagagttc
    47821 ttttttattc ccaaaacgac aaacaaacga ggtggtggta tgaatgaaaa ggcagattta
    47881 gcccatgaag attacttaaa agggcttaag tacaaggaaa tagctgaaaa gcataatgta
    47941 agtttatcga ctgtaaaatc atgggcaact agatactgga aacaaaaagg ttgcaaccaa
    48001 tcaaaaaaag ttgcaaccaa aaagagaggt gcacccatag gtaataaaaa tgctactggt
    48061 ccaccaggta ataaaaatgc tgaaaagttt ggtttcttct caaagtatct acctgaagaa
    48121 actagggaat tgatgcaaga aatatctata aaagataaat tcgatattct ttgggaacag
    48181 ataacaattc aatatgcagc aataataaga gcacaaaaga taatgtatgt taaagacaag
    48241 gaagaaatga ttaaagaatt aaagaaacat gaaagtacag aaaatggaga gaagatagag
    48301 tatgaatttc aatttgcatg ggataggcaa gcatcttttc ttaatgcaca gagtagagct
    48361 atgagtgaat taagaagttt aattaaacag tatgatgaaa tgattcataa ggattggaat
    48421 ttggctacag aggagcagaa aacaagagtt gagaagttga aatgtgaagt tgataaccta
    48481 agtaaagatg acattggaga tgatgagttg aaaataagtg tagattatgg tgatagaaat
    48541 gatagttaga gtaaatttta atccagattt caaggaagcc aattttacta aaaaaagata
    48601 cagagcaatg aaaggttcag cagggagtgg aaaatctgtt aatgtagcac aagactatat
    48661 actaaagtta ggagataaga agtatcaagg agctaatcta ttagtagtta gaaagtcaga
    48721 agctacacat aagtattcaa cgtatgcaga gcttacagga gctataaatc gtatttatgg
    48781 taaacaagct gataagtatt ggaaaactac tttaaatcct ttagaaatta agagtaaagt
    48841 tactggtaac tctataattt tcagaggagt taatgatgca aaacaaagag aaaaattaaa
    48901 atcaattaac ttctcgaaag gaaaattaac atgggtttgg tgtgaagaag ctacagaact
    48961 tatggaaagt gacatagata tactagacga ccgtttaaga ggtattttaa ctaatcctaa
    49021 cctatactat caaatgacat ttacatttaa tccagtctca tctactcatt ggataaaaag
    49081 aaagtatttt gactataaaa atgatgatat atttactcat catagtactt atctacaaaa
    49141 tagattcata gatgaggctt actacagaag aatgcaaatg agaaaagagc aagaccccga
    49201 agggtacaaa gtctatggtc ttggagaatg gggagaaact ggtggagcaa tacttaaaaa
    49261 ctatgttata catgaatttc ctacggaatt tgaatacttt gataatatga gactgtcaca
    49321 agactttggc tttaatcatg caaatgtagt actcagaatt ggttttaagg atggagagtt
    49381 atatatatgt aatgaaatat atgtacatga aatggataca tctgaaataa taaagattgc
    49441 aaacagtaaa ggtttagaaa agaatatatt tatgtactgt gatagtgctg aaccagatag
    49501 aattaagatg tggaagagtg caggatataa agctaaagga gttaaaaaag gaccaggaag
    49561 tgttaaagct caaatagatt atttgaaaca attaagaata catgtacatc ctagttgcac
    49621 taataccata aaagaaatac aacaatggaa atggaaacaa gatgaaagaa ctggattata
    49681 tcttgatgaa ccagttgagt ttatggatga tgcaatggct gctcttagat attctataga
    49741 taataagctt aaaaataatg gaataagctt cttaaagtaa aggaggcgtt aaatatttat
    49801 ataagtgaaa cagatttaat aaaagttcag ttaaaaaaag agagcac
//
LOCUS       DAYATN010000015        49103 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.015, whole genome shotgun sequence.
ACCESSION   DAYATN010000015 DAYATN010000000
VERSION     DAYATN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.015"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(130..324)
                     /locus_tag="ACGI8A_000852"
     CDS             complement(130..324)
                     /locus_tag="ACGI8A_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178862.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(633..2162)
                     /gene="lysS"
                     /locus_tag="ACGI8A_000853"
     CDS             complement(633..2162)
                     /gene="lysS"
                     /locus_tag="ACGI8A_000853"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFH0178863.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2177..2656)
                     /gene="greA"
                     /locus_tag="ACGI8A_000854"
     CDS             complement(2177..2656)
                     /gene="greA"
                     /locus_tag="ACGI8A_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFH0178864.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="ACGI8A_000855"
     CDS             complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="ACGI8A_000855"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFH0178865.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRATEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3870..4640)
                     /locus_tag="ACGI8A_000856"
     CDS             complement(3870..4640)
                     /locus_tag="ACGI8A_000856"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HFH0178866.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4659..5249)
                     /locus_tag="ACGI8A_000857"
     CDS             complement(4659..5249)
                     /locus_tag="ACGI8A_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFH0178867.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTATSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5432..6400)
                     /locus_tag="ACGI8A_000858"
     CDS             complement(5432..6400)
                     /locus_tag="ACGI8A_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HFH0178868.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6393..7370)
                     /locus_tag="ACGI8A_000859"
     CDS             complement(6393..7370)
                     /locus_tag="ACGI8A_000859"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFH0178869.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="ACGI8A_000860"
     CDS             complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="ACGI8A_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFH0178870.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIIVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="ACGI8A_000861"
     CDS             complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="ACGI8A_000861"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFH0178871.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDGEGIICVGDYKISENYKIDES
                     TKEVLKINFNKL"
     gene            complement(11260..11688)
                     /locus_tag="ACGI8A_000862"
     CDS             complement(11260..11688)
                     /locus_tag="ACGI8A_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFH0178872.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11705..12511)
                     /gene="murI"
                     /locus_tag="ACGI8A_000863"
     CDS             complement(11705..12511)
                     /gene="murI"
                     /locus_tag="ACGI8A_000863"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFH0178873.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12713..13198)
                     /locus_tag="ACGI8A_000864"
     CDS             complement(12713..13198)
                     /locus_tag="ACGI8A_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HFH0178874.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="ACGI8A_000865"
     CDS             complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="ACGI8A_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HFH0178875.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14053..14733)
                     /locus_tag="ACGI8A_000866"
     CDS             complement(14053..14733)
                     /locus_tag="ACGI8A_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0178876.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14717..15607)
                     /gene="ispE"
                     /locus_tag="ACGI8A_000867"
     CDS             complement(14717..15607)
                     /gene="ispE"
                     /locus_tag="ACGI8A_000867"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFH0178877.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15725..17275)
                     /locus_tag="ACGI8A_000868"
     CDS             complement(15725..17275)
                     /locus_tag="ACGI8A_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOCS domain-containing protein"
                     /protein_id="HFH0178878.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVECIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17369..17635)
                     /locus_tag="ACGI8A_000869"
     CDS             complement(17369..17635)
                     /locus_tag="ACGI8A_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFH0178879.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="ACGI8A_000870"
     CDS             complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="ACGI8A_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HFH0178880.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18727..19665)
                     /locus_tag="ACGI8A_000871"
     CDS             complement(18727..19665)
                     /locus_tag="ACGI8A_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HFH0178881.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19775..19936)
                     /locus_tag="ACGI8A_000872"
     CDS             complement(19775..19936)
                     /locus_tag="ACGI8A_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178882.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19946..20443)
                     /locus_tag="ACGI8A_000873"
     CDS             complement(19946..20443)
                     /locus_tag="ACGI8A_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HFH0178883.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20558..21268)
                     /locus_tag="ACGI8A_000874"
     CDS             complement(20558..21268)
                     /locus_tag="ACGI8A_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HFH0178884.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21444..21974)
                     /locus_tag="ACGI8A_000875"
     CDS             complement(21444..21974)
                     /locus_tag="ACGI8A_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178885.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21991..22221)
                     /locus_tag="ACGI8A_000876"
     CDS             complement(21991..22221)
                     /locus_tag="ACGI8A_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178886.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22236..23630)
                     /locus_tag="ACGI8A_000877"
     CDS             complement(22236..23630)
                     /locus_tag="ACGI8A_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFH0178887.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23756..25009)
                     /locus_tag="ACGI8A_000878"
     CDS             complement(23756..25009)
                     /locus_tag="ACGI8A_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFH0178888.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTIPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25060..26214)
                     /locus_tag="ACGI8A_000879"
     CDS             complement(25060..26214)
                     /locus_tag="ACGI8A_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0178889.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGISGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26774..27283)
                     /locus_tag="ACGI8A_000880"
     CDS             complement(26774..27283)
                     /locus_tag="ACGI8A_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFH0178890.1"
                     /translation="MYQQQQEILNKYNDAKIIKETYTGTTTNRPKFEKIISSLKKGDL
                     LVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLLENTSMGKFFLTTLLAVAELE
                     RNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYALGLLNSKPYREVVKMTGISKS
                     TLIRAKKSI"
     gene            27546..27773
                     /locus_tag="ACGI8A_000881"
     CDS             27546..27773
                     /locus_tag="ACGI8A_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFH0178891.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(27992..28735)
                     /locus_tag="ACGI8A_000882"
     CDS             complement(27992..28735)
                     /locus_tag="ACGI8A_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFH0178892.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28903..29775
                     /locus_tag="ACGI8A_000883"
     CDS             28903..29775
                     /locus_tag="ACGI8A_000883"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0178893.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29775..30440
                     /locus_tag="ACGI8A_000884"
     CDS             29775..30440
                     /locus_tag="ACGI8A_000884"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFH0178894.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30461..31318
                     /locus_tag="ACGI8A_000885"
     CDS             30461..31318
                     /locus_tag="ACGI8A_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0178895.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31474..32883)
                     /locus_tag="ACGI8A_000886"
     CDS             complement(31474..32883)
                     /locus_tag="ACGI8A_000886"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0178896.1"
                     /translation="MVTILISAYIPARKAANTPVMECIRQTNEVKIESKSVKTSKLTQ
                     RVYGLEGVLAMKNFKRNKKRYRSIVLSLVLSIVLFISTSALVADLKQMSNQRSEVTDQ
                     DIIFSTQGMKDNEMLKLYDKLKTANGVYEGLYQKLMTYSCVAKVSELTDDYLNYEGSH
                     SMSEMLELPVDMQFLDDRTYLNIIKSLGLPAEEYTGQNAKLIAVSMVTKDNNIIDIFN
                     SSSMNVSLTPETNGKLEIPERQNVNISFVKVVPPDSPPGIRSSDVKKASFRVLAPYSA
                     KDKFEIPGVSTGIESLSFQSKNPTQSVAEMEQIIESEGVTNEYNLYNMYGMMDENRNY
                     IFIANVFAYTFIIMISLISIANVFNTISTNIKLRRRELAMLRSVGMSEHDFQKMMNFE
                     CAFYGMRALFFGLPIAVISSWLIHKVMVTDDIDFVIPWFSIGISIFSVLFIVFITMLY
                     TINKIKKENIIDALRDDMA"
     gene            complement(32887..33225)
                     /locus_tag="ACGI8A_000887"
                     /pseudo
     CDS             complement(32887..33225)
                     /locus_tag="ACGI8A_000887"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            33188..33313
                     /locus_tag="ACGI8A_000888"
     CDS             33188..33313
                     /locus_tag="ACGI8A_000888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178897.1"
                     /translation="MASTMPPTEYKNILNKLLSETPMNRNTLSLKMEAWSLVLLV"
     gene            complement(33472..34020)
                     /locus_tag="ACGI8A_000889"
                     /pseudo
     CDS             complement(33472..34020)
                     /locus_tag="ACGI8A_000889"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34020..34706)
                     /locus_tag="ACGI8A_000890"
     CDS             complement(34020..34706)
                     /locus_tag="ACGI8A_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178898.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34789..35778)
                     /locus_tag="ACGI8A_000891"
     CDS             complement(34789..35778)
                     /locus_tag="ACGI8A_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0178899.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35765..36451)
                     /locus_tag="ACGI8A_000892"
     CDS             complement(35765..36451)
                     /locus_tag="ACGI8A_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0178900.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(36448..36570)
                     /locus_tag="ACGI8A_000893"
     CDS             complement(36448..36570)
                     /locus_tag="ACGI8A_000893"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178901.1"
                     /translation="MDYIRTKETTENCVMISKIVVYYCSAGIYKTNKIKEDESK"
     gene            complement(36951..40157)
                     /gene="carB"
                     /locus_tag="ACGI8A_000894"
     CDS             complement(36951..40157)
                     /gene="carB"
                     /locus_tag="ACGI8A_000894"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFH0178902.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(40350..41399)
                     /gene="carA"
                     /locus_tag="ACGI8A_000895"
     CDS             complement(40350..41399)
                     /gene="carA"
                     /locus_tag="ACGI8A_000895"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFH0178903.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(41656..44862)
                     /gene="carB"
                     /locus_tag="ACGI8A_000896"
     CDS             complement(41656..44862)
                     /gene="carB"
                     /locus_tag="ACGI8A_000896"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFH0178904.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(44915..45958)
                     /gene="carA"
                     /locus_tag="ACGI8A_000897"
     CDS             complement(44915..45958)
                     /gene="carA"
                     /locus_tag="ACGI8A_000897"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFH0178905.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(45984..46700)
                     /gene="pyrF"
                     /locus_tag="ACGI8A_000898"
     CDS             complement(45984..46700)
                     /gene="pyrF"
                     /locus_tag="ACGI8A_000898"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFH0178906.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(47076..47711)
                     /locus_tag="ACGI8A_000899"
     CDS             complement(47076..47711)
                     /locus_tag="ACGI8A_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HFH0178907.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(47735..48649)
                     /locus_tag="ACGI8A_000900"
     CDS             complement(47735..48649)
                     /locus_tag="ACGI8A_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0178908.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
BASE COUNT        15288 a         9243 c         5393 g        19179 t
ORIGIN      
        1 tttcgtcaat actttttttg ttttttattt taaaaaaggt tataactttt ggttttttta
       61 ataaatataa ttaaaactaa tagatataac ccttgatatt acaaacaata catgcatata
      121 taattttacc tattttgcaa ttttattttc ataagaatat gaattagctc tcactctatc
      181 tgcccaaaca tttatactct taactttatt aacattagtt gtaccctcaa aaatatccat
      241 tgcatcctta caaaaatata tcataacatc aagaaccaaa aatccaacac aaaataaaca
      301 taaaaaatta attacttgaa acatcagaca ttcccccaat aattttttat atatagtatt
      361 ctcataaatt attcttttta ccttctcata aattattcta aatattttca taataaatta
      421 ctttcaatca tattttctaa atataaagtc tcaatcaaca aaaactaata tcaatttcta
      481 acgtttgttg gtaatttata aattaaatat ctaaaagaat ctttgaagca tataaaaagc
      541 attccacact gacattttaa ataaaattta gctatttatt gataatttaa tagctaaact
      601 ttatttaaaa cttagtagaa tgctaatatt tcttagtttt cttcttcttt atttggattg
      661 ttatcaatag gtttcatagt cgggaataat aatacatctc ttatagatgg agagttagtt
      721 aataacataa ttactctatc tatacctatt cctagtcctc ctgtaggagg tagacctacc
      781 tctaatgctt ttaagaaatc ttcatccatt tcaaaagctt catcatcacc taattctctt
      841 tttctcaatt ggtctataaa tcttcctttt tggtcaatag ggtcattaag ttcagaaaat
      901 gcattagcta actcccactt attagcaaat gcctcaaatc tatcagttct tcttgggtct
      961 tcaacatttc tcttagataa tggagatact tcaactggat ggtgagttat aaatgttggt
     1021 tggattaatt tatcttctcc aaattcttca aagaaagcat ttattacttc tcctcttctc
     1081 attccaggtt ttatctctat acctttttct ctagcaactt ctagtgcctc ttcatctgta
     1141 tttatagtag aaaaatctac accagaatat tctttaacac aatcttccat actcattctt
     1201 ttccaaggtg gtgtaaagtt tatttctgtt ccttgatagt taactatcat acttcctgta
     1261 gcaacttcag ccatatgtgc aataacattt tctgttattt ccatcatatc agtatagtct
     1321 gcatatgctt gatataattc tattgcagta tattcaggat tatgttttaa atccatacct
     1381 tcatttctaa acattcttcc catttcataa actttatcaa atccaccaac aataagtctt
     1441 tttaaataca attcattcgc tattcttaaa tacattggta tatgtaaagt attatgatta
     1501 gttataaatg gtctagcatt tgccccacct gctatagtat ttaatattgg agtctcaact
     1561 tctaaaaatc ctctatcatc taagtatgct cttaatgctt ttaatgcttt tgttctaatt
     1621 aaaaaagcat tttttacttc tggatttact attaagtcaa catatctttg tctatatctt
     1681 agttcttggt cttttaatcc atgatatttt tctggtaaaa cttgtaaaga tttacatagt
     1741 aatactacag atttagcctt tacagatatt tcaccttttt tagttttaaa gatttctcct
     1801 tcaattccaa ctatatctcc aatatcatat gttgaaaata cactatattc ttcctctcct
     1861 atagcatcta gtcttacata agattggatt cttccctctt ggtcttgaat atctataaag
     1921 ccagctttcc cttgtattct cttgctcatt atacgtcctg ctatttttgt agttttaccc
     1981 tcaagagaat caaagttatc ttttatatcc atagaataat gagttctatc atatctactt
     2041 tctttaaaag gatctctacc tgactcttgt aattttttta gtttatctct tcttacttga
     2101 agtacttcac taaggtcttc ttgaatttgc gcttcttcat tgctttgttg attatttttc
     2161 atttttttcc tccaaattat cttcttattt ctaatatttc aaacttagca actccatcag
     2221 gaacttgcac ttccacaact tctttcgcag ctctacctaa aagtgctttc cctacaggag
     2281 attcgtttga tattttacca tcatatgggt ctgcttctgt cgaaccaact atagtatatt
     2341 caacctcttc atcaaattct aaatccttaa ctttaactat tgatcctatt gtaacaacat
     2401 taaggtctat tttgctttca tctatgataa cagcttttct aaccatattt tctaatttta
     2461 aaattctttc ttcaacttgt gcttgttcct ttttagcttc atcatactca gcattttcag
     2521 ataaatcacc aaaagatata gcaactttta ttctctctgc aacttctttt ctctttacaa
     2581 cctttaagtt ttctaattct tcttctaatt tatcatatcc ctcttgtgtc aataaaaact
     2641 ctttattttc ttccattata aatacaactc cttcacaaat ttcttaggaa ttttcctaat
     2701 tatattttaa aaattttata atcttttttt aatttatgcg ctatattata agtcactatt
     2761 atttttatgt caaggagtct tccatatagt actccaattt cgacacaact tcttgatagt
     2821 cttctatttt gttaatttca tcccttaatt ttgcagagtt tctaagtcct tttaaatacc
     2881 aagcgatgtg ttttctcatt tcccttacag ctacatactc tccatgttct tctactgcaa
     2941 gttttaaatg cttaatggca gtacttattt tttcgttaag agttggctct ggcaaaatct
     3001 cgcctgtatt catataatgg tttattcttt taaatatcca agggttacct tgagcacccc
     3061 tacctatcat tatagcatca caaccagttt tgtctaacat atttattgaa tcttctattg
     3121 taaatacatc accatttcct ataacaggta tactcaaatt tttcttaatc tctgcaatta
     3181 tatcccagtc tgctttgcct gtataaaact gttctctagt tcttccatgt attgctaaag
     3241 cacttattcc acaatcctct gctatctttg ctatttcaac agcatttaca ctattatcat
     3301 cccagccttt tcgaatcttt aaagtcactg gcttttttga gttcttcact actgccctca
     3361 atacctcttc tgcaagctta ggatttttca taagtgctga accatctcca tttttaacta
     3421 cttttggagc tggacacccc atatttatat cgagaatctc atttgaataa tcattcatta
     3481 tctctgttgc tcttcccata aactctggtt ctgaaccgaa tatctgaact gctacaggat
     3541 gttcttcctc ttctatctta agcatttttt tagtattttc atcatcatag caaagtgcct
     3601 taccatttat catttctgta tataaaagcc cacatccttg ttctttacaa attaatctaa
     3661 atggaaggtc tgttacacct gccataggtg atagaaaaac tttatttttc aattctaaat
     3721 ttcctatttt cattgttttt ctcctatttt tgcctttatt ttgtgattta actttaaatt
     3781 cttatttcct cattataata taccacatat agctagaaat ctatcataaa aagggaattg
     3841 gatatataac ccaattccct gtatattaat tattcttggt ttttttcata tattattctc
     3901 aatccttcta gtgttaaaaa accatctaca taatctatac ttttcgtctc tgaagcaatc
     3961 agtttagcta atccacctgt agctataacc ttaacatcat cacaattcaa ttctttcttc
     4021 atcatactta ttattttgtc aactaaacca acatatccat aaattattcc agattgcatg
     4081 gctgatacag tactcttaca aatagtcata cctggcttag ctaattctac tctaggtaat
     4141 ttagacgcac tttgaaataa cgcctcacta gatattttta ttcctggtgc tattgttcca
     4201 cccaaatatt cacctttttc agagatagca caaaatgtag ttgctgttcc aaaatcaaca
     4261 agtatacttg gtgctccata cttttctatc cctgctacag cattaactat tctatctgcc
     4321 ccaacttgtt ttggattatc atattttata tttagacctg tttttatgcc tggacctact
     4381 attaatggct gttttttaca gtactttata caaaagtttt caagagaatg cataacattc
     4441 ggaactacag atgatattat aacatcatca atatcactta tattcacatt atcataatca
     4501 aataggttac ttatcaggat tccatattca tcagacgttt tttccctatc tgttttaatt
     4561 ctccagtaat taactaattt gtcaccttta tatataccta aaaccatatt agtatttcca
     4621 acatcaaata ctagaagcat atgtcattaa ccttctttct attttttgct tttcttaagt
     4681 acagtggcaa cagcagctac tactaaagcc cctactattg cttctggcac cccatttgct
     4741 gcggcaagag ccattattac ttttcctgct gaaccacttt tacccattgc atgaagatac
     4801 ttgtcagcat ataagacata tatcattcct aaaactccag ctgtatttaa cagagaacct
     4861 attgcccctg taattaaacc tgatatgtat accttatttg tagccttaat tattaactga
     4921 tatacataat aagctaatac acctatcata actcttggta atattgaaac taatggattt
     4981 ataaaaacaa aagatgttgc tgttgggttt gttatagcat ttaagagact tgatatccca
     5041 aatataaatc ctattgctgc cccaactatt ggtccttcca taatagctcc aataataact
     5101 ggtatgtgca ttatagttgc ttttgtaggt ccaactggaa taaatcccaa tggagtcata
     5161 gatagcataa tagaaatagc tgatagtact cctattatag tcatctttct tacatttact
     5221 tttcttactg atgcacttgt gttcatcata aaaaatacac ctccgttcca gctcattaag
     5281 atgccggact acttaatttt ttaatttttt tcgttttttc ttataactat gagtcaagct
     5341 accattgtag gatgcccgct ctaacaagaa taattttatc aagatgagtt gccattgtca
     5401 acaatttgac aagaaaaata tgctaatttc atcatatttc tcgttttttt actatatctt
     5461 tatatgccat tatattttct acactatccg ctttttttac tgcatttatt atactttttt
     5521 ccacaacctc tacagccaat gaacctacta atgttatatc tgcttctatt tctcctgttg
     5581 atattgtgaa tattgtatct ccatcatgag gtgtatggat aggaaaaata gacttagcat
     5641 atccatcatg tcccatttga gatatctttt tacatccagc tttggttaac ttagcatttg
     5701 ttgcaattat cccaattgta gtattatcta cactaaatcc acctttactt tcaccttgtt
     5761 tcattaattt atatgtatca attgtttctg tctttttatc atttaaagca ccagctataa
     5821 ccttaccatt ttcataaaca tctccaagag catttacagc aattaatgca gatacaatta
     5881 gaccattatc taattttata gaatagctac ctattccgcc tttcatagca tattcctgac
     5941 ctcttatctt tccaacagtg gctccacaac ctgctccata atttccttga tttaaaatat
     6001 cagtatttgc aacttcacaa gctctataac ccatctttaa atctggtcta catttaaagc
     6061 tccctactcc taaatcaaat aaaactgctc ctacaactat aggcacttta cctacaccta
     6121 catcaaagcc aattcctttt tcttctaagt atctacttac accacaagtg gattcaagac
     6181 catatgcaga acctccagcc aaaactactg catgaacttt ttgaaccata tttacagggt
     6241 ctaataaatc ggtttctcta gtacctggag cagagcccct tacatcaaca ccgcaagtag
     6301 ctccttcttc acatattact actgtgcacc cagtaagacc atttctatcc tcaacctgcc
     6361 caacctttaa gccttctata tccaaaatat tattatacat atcctttttc tcctcttata
     6421 gaaacttcac ctgaaataat ttcttttata cttccatcac ttctttgaac taataaattt
     6481 ccctcttcat ttatgtctaa acatttcact agttctctat tatctccttt taatacatat
     6541 atatttttat ctataattgc agaataatct ctacatattt ttaaggtttt ttctttggaa
     6601 ttatttacag tataatctaa atataatttt tcaaattcag ttaatacatt cctaacaata
     6661 tctactctgg ataacatata tccttcttta tacaatgatg tggctttgtc agctatttct
     6721 tgttcaaaat ccattatttt aacatttatt ccaattccaa gaacaacata atttgttctt
     6781 tcaatttcag cagataactc tgttaatata ccacatattt tttttccatt taaaataaca
     6841 tcatttggcc attttattaa tgtatttgct cctagattgt ttaaagcttt cgttacactt
     6901 gctcctgcta ttaaagttat aaatggggct ttgaatggtt ccatatctgg ttttaatata
     6961 atactcatcc agattccttc atgaggtttt gaatcccatc ttcttcccaa tcgtcctttc
     7021 cccatatctt gatgttcact taaaatcaca gtcccatcta cactatttat agctatcctt
     7081 ttagcatgtt catttgttga atctatagtg tcaagatgtt caatattttt tccaataaac
     7141 tctgtatcta atttatagta aatattctgt gggcttaata aatcagttgg attttccgct
     7201 aatcgatacc cttttttatt tacagattct atattatatc cctcttcttt taaggctttc
     7261 atatgtttcc atatagctgc acgtgacacc cctaattgtt ttgataattc ttctcctgaa
     7321 ataaattcag atttattatt taatataatc tctattattt tatctctcat atttcacctc
     7381 taatatgaca taatttcatg acttattttc ttctaataaa taattataca ttattaatat
     7441 acttttaata ctattaattt taaatttcct ttttaattat tataatggtt gttttatatt
     7501 ttatattgat gatttatata attttacatt ttatttaaat aattttaaaa taataaaaaa
     7561 actgaaagta tcaattaaat taatacatgc aaatataatt tgcttattaa tttaatatta
     7621 tactttcagt tttagtattt aatccacatc agataataat cttaaaatga ccaaggtctt
     7681 aaaactattt atctttatca gatttatctt ctaaattttt atttatatca ataacattgt
     7741 ttctttcttc ttcatctttt tctaatttta tagttaattg ttcttcaact tctttagaag
     7801 aattttcctc tgtaactgca ttttcaattt cattcagagg taactcttta ttaaatgctt
     7861 ttacaaattg ttctgcatct aaagtttcat atataagtaa agcttgtgct acatactcta
     7921 atctatccat attttcttgt aatatacttc ttgtcttatc atatgcacca tcaactatac
     7981 gctttgtttc ttggtctatc tcaaaagcaa cttcttcaga ataatttctc ttacttgaaa
     8041 aactattacc taggaatact tcatcatcac tgtcaaatga cattggacca agtttagaac
     8101 tcattccata ctttgttacc atacttcttg cagtagctgt aactctttct aagtcatttg
     8161 aagcacctgt agatacatcc ttaagtacta gctcttctgc tactctacca ccaagtaaaa
     8221 ctataatatt ttctttcatt tcattttttg tagcatagaa cttatcttca acaggaagtt
     8281 gcattgtaaa ccctccagct cttcctcttg gaactattgt aacttgatga acaggactta
     8341 cttcttcaag tacatgggca caaactgcat gccctccttc atggtatgct gtaagtcttc
     8401 tttctttctc acttattact ttcgattttt tagccacacc tgctataacc tttgttatag
     8461 cttcttctat agtttccatc ttaattttct tttctctttt tctagctgtt aatattgcag
     8521 cctcattcat taagttctct atatcagcag gagtaaatcc tggtgttctt ctagccaaga
     8581 catccatttt tacatcatca cttaatggtt tatttcttga gtgaacttta aatatagcct
     8641 ctctaccttt tacatctggt gtaccaacaa caacttgtct gtcaaatctt cctggtctaa
     8701 gtaaagctgg gtcaagtata tctggtctat ttgtagctgc catgattatt ataccttgat
     8761 ttaccccaaa tccatccatt tcaactagaa gttgattaag agtttgctct ctttcatcat
     8821 gacctccacc aagacctgca cctctttttc taccaactgc atcaatttca tctatgaata
     8881 tgatagctgg agcacttttc ttagcctgtt caaataaatc tcttactctt gaagcaccaa
     8941 ccccaacaaa catctcaacg aaatcagaac cacttatact gaaaaaggga actcctgctt
     9001 ctcctgcaac tgctcttgat agatatgttt taccagttcc aggaggtcct accataagca
     9061 ttccttttgg tattcttgca cccaattcaa tatatttctt aggattcttt aagaaatcta
     9121 ctacctcctg caaatcttct ttttcctcat ctagtccagc tacatcttta aatgtaactc
     9181 tagttttttc atcatcttta tgaaccttag cttttgattt gccaaaactc ataacctttc
     9241 cgcctccacc ttgagactga ttcataaaca cgaaccaaat aatcaccatg aagaatatta
     9301 acagtaatgt aggcaacatt tcgacaaacc aaggtgtaga tggcttagct tcaccaccaa
     9361 aagttagttt cccagcctta gcttggtcta acacctcatc tgcaagttta tttcccatca
     9421 cttctgttgg aacatatgac ttaaactttg tatttgtatc ttttatagta ccttctacag
     9481 aagtttgatt tacaaagtat aatcttgata tattctcatc tgttaattct ctatatactt
     9541 cagagaattt taaatctttt attttttctg taggcttacc tgagaattga actataccta
     9601 ctataattat aaaaacaagt agataaaaac ctgccccttt tagcaactta ttcaatatag
     9661 aagccctcct ttcatacatt gctttgccct ataatttgtt aaaattaatt tttaatactt
     9721 cttttgtgct ttcatcaatt ttataatttt cactgatttt ataatctcca acacatatta
     9781 ttccttcacc atctgtgatt ataggaattt tacatctttc ttctcttggg atttttaaat
     9841 ctatgaataa atctttcact tttttacttc ccatagcaag ctttatctta tcaccttgcc
     9901 ttctgcttct tattactata ccccctttta ccttattaaa atcaaaccct ttagatgaat
     9961 tgtctaattt catactctta tacctatcta tgctcattac ttgggtctca ataatttgtt
    10021 ttaattcttt tattttaata aacccattac taggtacatt gtaataaaat tcaatttcct
    10081 cattaacaat ttcctcattc gttaatatta tactgtcttt ttttctataa acaaatatat
    10141 ttcttggaag agttaacatt ttatttactt ttgaattatc ttctaaagac attatatctt
    10201 ctatatgtct ttggtcaaca aaattagtat ctcctagtat atgttttatg gaatttctaa
    10261 gaactctaac tttgatagca tcatgtaagc aaacaaaatc gtctaaattt atctctacaa
    10321 agcctttttc ttttatattt gaaacttctc taaatttagc ttctgcctct ttctcaatat
    10381 aatcgttatc actttttaaa ctattgctca tcctcactat agattctatt acatttgaat
    10441 taaaattatc cttcatatat ggtaaaaggt ctaaccttat cttatttcta gtgtatatat
    10501 tttctaaatt tgttttatct atcctcggat ttaaattata agcttcacaa tactcctcta
    10561 tctcatttct ttccacgtct aatatgggtc taattatgca attatctcta atataatcaa
    10621 ttccctttaa gccttttaaa cctgttcctc tcatgatacg catcataacc gtttcggctt
    10681 ggtcattgag attatgacct attgctattt tattggcttt aatcttatcc ttaatttcat
    10741 aaaacatttc atatcttagc ttccttgctc cttcttctaa tgataatccc tcattttcac
    10801 aatattttgg tacattaata gactttacaa agaatataat tcccatatcc tcacaaagtt
    10861 tagatacata taatgcatct ttttgagctt ctatccctct tatttgatgg tttaaatgtg
    10921 ctgcatatat ttctatatta aaatcttttt ttaatctatt tagtacatgc aataaacaaa
    10981 ctgaatcagg tcctccagaa agacctaaaa ctattttatc gcctttttgt attaaattat
    11041 gtttatttat agtacttagt accttatcaa atatcatatt tatccctctt acacttagtt
    11101 ttattcactt ttaagtatac tttctataaa aaaacgctaa acccttgtat caattttaag
    11161 aaattaaagt ttaatataaa tataataata ccaaataaat taaataaaag gtagtttttt
    11221 cactaccttt ataaaatttc tggaaaatag ccatagttat tatttagtac ctatgtatat
    11281 atctatttta ttcctaccta aaaataaatc ttgtaccttt atattatagt ctattttcaa
    11341 ttttatgtgt tccccttctt gtatatttat atctaattct cttgtatcaa tatcaattaa
    11401 taaagtacct tgtggggtca catactttgt agtactactt tctccacatt taaacagcat
    11461 tgttgagtta atagcaccaa actttttgat actaacttga tttttaagta tctttatagt
    11521 attagttatt tctatatctt cttctttttc cttgtaaata acatatattc catcattttt
    11581 ataaaaaatt ttaccaaagg cagtcatctc tatggtgtcc atatttcctt tttcatcata
    11641 ttgtctagta tttatactta tttttacact caacgatttt tcctccatag attcttcata
    11701 taaactagta tttttgtatt tctacttttt gaatcttatc aatttttttc tttaagaact
    11761 ctcttgctat atctgaaaat ttttcaggta tatctgatac ataatattga taagttccat
    11821 gaatttctgt attgtttata atattttgca cctctaaaat cttttttaaa tcctttgcag
    11881 tctctttagc tggattaaca agttttatat gctctccaac ttcttcacct attgttctct
    11941 ttaaaatagg ataatgagta catcctaaca ctaaagaatc tatatttttt tctttcaatt
    12001 cttgtaaata tatctttgct gtcagtttag ccacttctgt atttgcccat ccttcttcaa
    12061 caataggcac aaataaagga catgctttat taacaatttc tatactctca tctattttag
    12121 atatttccaa gttgtatgct tttgaactta tagttccttc agttcctatt attcctacaa
    12181 ttttgttttt tgtagaactt acagctgttc tagctcctgc ttctataact ccaattatcg
    12241 gtatatcata tttttcttga gcttctttta agcttcttgc agtagcagtg ttacatgcta
    12301 taactatagc ttttacacct tttgttttta aaaagtttat agcttgaaaa gtatatttaa
    12361 ttatagtttc tttggatctt gaaccatatg gtattctggc agtatctcca aagtatatta
    12421 tatcctcatt tggtaatatc ttcattatct cttttagaac tgttaatcca cctaatcccg
    12481 aatcaaaaac tcctattggt ttattgttca tctttataca acacctactt tacatttttg
    12541 tgtttaacaa caacttattt atcgctaaaa tttatattta tattcataat taatatatta
    12601 ttacctaaat gattattata gtttatttta cacaaaaaat aaaggagcta tcttaaatta
    12661 gaaaataatt tctacaaaaa gacaactcct ttcactactt aaaaatttaa atttattttg
    12721 caaacacatg atttcctatt ctcatataag gattcaatct atttatccat ttgcaagtgg
    12781 ctatttctgg attccaaaaa tatactgctt tattagttgg gtcagttcca taaagagctt
    12841 cctgagctgc tttacgtgct gagtctgttg gtgccatctt tattgaacca tcaagcacaa
    12901 ctgagaaagc atttttttga tatatgactt cttctattgt gtctggaaat ctagggtcca
    12961 atactctatt tattactaca gctgctacag caacctgtcc ctcatagctt tctcctctag
    13021 cttctcctgc aaccaactta gataataaca tcaagtcatt atcagataaa tttattactt
    13081 ctttctttgc tatatttttt tcttgtttgt ttactttctt aatattttct gttggttcat
    13141 atgcaaatac ctctttaata ggcataaata taaaagtcaa agctaatagt gtagccatca
    13201 gtttactcca cctgtccatg attaaaacca ctccttacat tcttattttt aaaatttttt
    13261 ctctaaatcc ttgactactt caagtacttt aaacttaatt tttactctac ttgttttttt
    13321 tggtgtatct tgttttatta acttatattc tttatcagtt aaaacttcct tcaacttatc
    13381 atctgtagat ttatctatca ctccattaga ttcatcaaca aaacatagtg gagggaacat
    13441 aacacaccac cagttttttc cttcaccttt cccaataata atctttaatg ctttatattc
    13501 accagctgga agaactatat tagaatattg tttagtaggg aaattgctat attgtatacc
    13561 tacctttaca ctgtaattat atccattttt aagaataata tttttactta tttcttccag
    13621 actactatat tcctttttta taatagcttc actttctttt atactttttg aattttgaag
    13681 ctttggctgt aaatatttta ttacttcatc tctaactttt agtttaagtt cttggtcttc
    13741 atctgtattg ctgtttgcta taacatgaaa tcttattaat ttatctttat aatcttttga
    13801 tatattgtct acttttttta cctcaccatt tattactatt gtcattattg atatgactga
    13861 tataagactt aatattaata gtcccaatct aatcttaatt tttctcattg aaaattcccc
    13921 ctcaagataa atttatatca tcaattatgt ccaattaaat ttaatttatt cataatattt
    13981 tttaatacta ataaaaaaag ctatcttaaa atgattacaa tcattttaaa atagcttttt
    14041 aaaccttata ttttataagt ctttactaat catagcctta tctatcataa actgctctgc
    14101 tttttctata tgctctgtag catacttctt ggatagctca ctatcacctt taattatagc
    14161 atcaagtaaa tatttatgct cttcaacaat attatgtgat gtatcataag cagacatata
    14221 ggtaactcta aatctatata cttgttccca caatgagttt attaattgga ttaatttttt
    14281 attattagtt gctttaaata tacattcatg aaattctaca tcaagtttta ataacgcatc
    14341 tacatcggat tccaaagtgc tttttttaaa ttcttctgat atatctttaa gctttttaat
    14401 atcttcatca ctaattcttt cagcggcaag atatgctgct aagccttcta ggcttgctct
    14461 tacttctaat acatccataa tatcctttaa tgacatattt gccacatacg caccttttct
    14521 aggcaacata acgactaaac cttctaattc aagctttctt attgcctctc tcactggagt
    14581 cctacttact cctaattgtt ctgctagttg cacttccatt agcctttgac caggctttaa
    14641 ttttccttct aagattgctt ctcttaaatt ctcaaagact acatctctta atggcttgta
    14701 attatccaag ttcagtttag ttaaattctc cacaaatttc aacccctttt tgactgctat
    14761 taacaacata cacctgttta tattttttta ataattcttt ttttccaatt agagcatctt
    14821 ctttattttt aaatagtcca aaaactgtag gaccacttcc actcatcata gaacctaaag
    14881 cattattttt catcattact tgttttatat cacttatttc tttgtgttta cctatagtaa
    14941 cattttcaag aatatttacc atgctttctg atacagcttt tatatcttca gattttaaac
    15001 actctatcaa atatttatta tttggtcttt ttttaatatt attcaaatct aatccttgat
    15061 aaacttcttt agtagaaacg aataagtctg gtttgcatat caatatattt gtatcacatg
    15121 gtaaaccttt gatatttgtt agcttttctc caataccttg agctaaggca ggtcttcctg
    15181 atatacaaaa aggaacatct gcgcccaaat taagtcctat ttcttttaat tcatcttcag
    15241 ataatctaag ttcccatagg tgatttaaac ctactaatac tgcggctgca ttagaacttc
    15301 ctccagccat ccctgctgct acaggaatat ttttttctat gaatatttct acaccttttt
    15361 ttatataaaa tttattttta agtattttgg cagctttata aacaatatta tcttcatcta
    15421 aaggaatatc taaactagtg ctttttacct ttatttcatc ttcgtctaat tcttttatct
    15481 ttactatatc gtataaatct atcgtttgca ttatcatttc aacaaaatga taaccgtctt
    15541 gcctttttcc caagacatca atggataaat ttatttttgc tctactcttt agtcttataa
    15601 aatccattct atcctccttg tttaaagaat aatgcatcac taacaataat attatactac
    15661 aaaaaataaa ataatagggt attataatac cctattatac ttaattactt taaatttttt
    15721 aatattaatc tactaatacg actttttttt ctaaaataag acacttccca ggcttaattg
    15781 gttcatcaag ttttatatca ttaagttctg caatttcatt ttctgttgta ttgtattttt
    15841 ttgctatatt ccagaaagta tctccttctt tgcatatata tacgattatg ctaggagctt
    15901 tagataaatc atatacacct tggtcttctc ccttgataat aaaattttct gctttcttat
    15961 ctaatgcttc agtaaatctc ttaattttga taatcaagtc tatctggtcc ctattcaaat
    16021 caacttcgac tttatcaata catgctgtat taaacacaga tgctgtatca gttagattat
    16081 ccatttctat atcatgttca aatggaattt cctcactaat tttataaacc aattttaaac
    16141 cttcaactgg gacaaataaa atttcaactt ttatgattcc ttgtataatg cttttatttc
    16201 cttcaatata actattttct atagacatgg ttggacaaac gcttacaata tcttttattt
    16261 gtatatcatc attatcattt cttatgcctt ctctaaccat aaatgtttct tcactacgtc
    16321 ttaaagtctt atttaattga ataggtttgt ggtcaaattt tattatcttt tgaggcgaat
    16381 atgcatcttg taatacttct cttgtaactt catctgtaac ttttacctta caacaagcca
    16441 tacaatctat ttctaaaaga cctgtgttgc tttcactatt ttgcttgaaa atatgattaa
    16501 aatctgacat agacaacaat acttcttcag tcataccatc actaactcca gggacttcta
    16561 caaattgagt aaactcaata cctactctat ccaactctac aagttcaccc tcatatgtac
    16621 aagctaacgg atttatttct aatactccac caataataac tttattgtca gtcactctac
    16681 tttcttttac tttaacacat ggatttaaac ttattataga ttgaatttcc tctgtgttta
    16741 ttgttatagt atctcttata gaactttctg ctttttctat tccaactata tcttgaaaac
    16801 aaatttcctt tctgtgcttt tgtatgcatt ccacttgcgc cacatctttt acaatgtcta
    16861 acctttgctt ttcaaacaaa cttcccctta tattcataag agctcctact ttaatctttc
    16921 tctcattcat tatagtacaa tccatatgct ctacttctga aaataacatg tattccatat
    16981 cttgaataac attgtctttt tctattactt cgtttatatc tacttttccg tctacattag
    17041 aaatagtatt tttatcatct gcaatataga ttacattata attaaaacta cctctgcaaa
    17101 gtatcttccc atctgccatt tcaatttttt taagtgatat gtatccttct gtttttacaa
    17161 tttcatatac atctaatttt ttgtctggca caactgcctc cgcttcaatg aatgtttgaa
    17221 attttccaaa gtctattcta ttgtcaactt taattacatc tttaattaat tccatagtct
    17281 acctccttaa gtattataaa aatatcacac tatatttata tttaaggagc aaaaaaaaaa
    17341 ttacacctat ttttaaatag gtataatttt aacttatttg taatttatct tgatctctaa
    17401 aaacttgcaa ctttacatta gatgttaata aatctgagta actataagat actctaggat
    17461 atccattact ttcatcatct aactttataa caaacacgct tggataaact ttttctagta
    17521 taccctcttt tgtaataatc tgctttcttc ctttattagc tttcaacaat attttctttc
    17581 ctatatgtct ctctaagctt actcttatct tatctagagt ttgaacagta gccacaatat
    17641 caccttcttt aacgtttata tgtttacata atatcacata taaacatttt tgtcaaatgt
    17701 ttaaactaat tattttatat attttttgca aaaatgtcaa tactattttt gaagatatac
    17761 agtataatta tgttaatttt tgctaatgta atattgtttt tggcattgcc catctaaatt
    17821 ttcctcttgt ttcagaacca ccaattccct taacacctgt aatagtctgc tctataacct
    17881 catcaatagg aactactaca tcaattcttg aacaagcaat tttctccact ataaaaacag
    17941 ggtctaatgc atgtatcatt attcttccag gcgaacttgc atagtttgct cctgcactta
    18001 ttattttttc ataatttgat tggcatgctc ctgcgaatat gacaagatta tccaaatttg
    18061 gttgccattt acgtgcttct tttactgttt taacaaaatt tgaagagttt ctatagttac
    18121 tcatatcttc tatatctcct ctttttattg tcattgcatc atgacctgtt attactagta
    18181 tatccggatt atatttttct agtagtgctc ttacttcttt atactgattt tgctctgaaa
    18241 cagctacacc tactgcaggt attccaagtt gagcatatac atccaagcaa atttttaaat
    18301 attctttatc tccatctatc tgtagtactt ttccaggcat tccatatgtg ttaggattta
    18361 attgaagttt tggaactgct ctagtcattc ttttttgtct ttctttagct tttcttactg
    18421 acttatataa taaattttct acatttttat ctattaatat atcttttata tcaggtgctt
    18481 ttactaattc taggtcatca atataagcat ctgctattac tctaaatgca attcccttta
    18541 gtatagctat tttctcatta ttttcatcta caccaaaaga gactatttta aatactatat
    18601 ctttgttatg tgactttcta gctacaatgt ctcctacctt catagttaaa cctccaataa
    18661 atttttatct tagtttatac tatgttcgat ataaaacttt gtttcctaat gtcaaaaaaa
    18721 catttcttat aatacttctt ttatcactct taaagcattt ttatagtata tcttttctat
    18781 atcatcttca ctaaaccctt ctttttttag aggttcatac agtttaccca tctgagatat
    18841 atcttcaatc tcaacttttg aatctattcc atcaaaatct gaccctagag atacaacatc
    18901 tattcctcca acatttacaa tatgttttat gtgtctaacc atatcttcaa gtttactttc
    18961 acttttatca cttaaaaata agtggaagaa gtttatacct gtaactccac ctttatttgc
    19021 taatacttta atcatatcat cagttaaatt tctagaatga ttcatcattg ctcttgaatt
    19081 agaatgtgta gcaattattg gtttagaaga aatctttgca atttcataaa atccaccatc
    19141 tgaaatatga gaaacatcaa ccaacattcc caattcattc atcttatgta ccacttctct
    19201 tccaaaatca gtaagccctt tttctctata ttctacttta ttatgaggaa agctcaattc
    19261 attcacatga ttccaactta ttgtcatcat tctaacccct aagtcgtaaa atttctttaa
    19321 attttctaat ttaccttcaa gtactgcacc ttcctcaata gataaaagag ctgtaagttt
    19381 accttctgat tggtttttca taatttcttc atagttagta gctaaagcta tttcatcact
    19441 attttttttc atttcctcat gaaatttatt tgccatactc atgcaataat caaaaggatg
    19501 ttttacttct tcagtatcaa caaaaagagc aaatgtttga gctaatgaat cccccttttt
    19561 caatctgtct atatcaacag aatatttatt agacttaagt tcacttgttt ctacattttc
    19621 cattaatttt gctatagtat cacaatgtaa atcaagaaat ttcatattta taccccctgt
    19681 atattttata tttatatcta aaaatagaaa tagttgtcct tatagacaac tattgacaac
    19741 tattttttaa tattcttttt aatattactt aatcttattc ttcttttaag ttaggtatta
    19801 cttttttaac ttcttcaatt tgatgccatg ttttatctat attattttca ctaatatatt
    19861 tttttaaatc agttaaagat gtaaaatctt taactgataa attgtcatca tcatctgcaa
    19921 atcttaaaat aaacatgaga ctcccttact ttccacaaca tttcttatat ttttttccgc
    19981 ttccacatgg gcatgaatca tttcttccta tttttgcttc tttaactatt gttttagact
    20041 tcttataagc ctttattatt tcttttcttt ggtctgctgg tagtatatct tcccaacctt
    20101 gtaaattata tagccaatga gcctcaacag ttaccatatt gttatataat ttttcccaat
    20161 ctatttttat agaaacttct ttatcagctt ctacttcttc taaaactata tcttctttta
    20221 agctttcact tattccatct atgaatccca tgaaaaattc attagtagtt ccaaattttt
    20281 cagccaattc acttacttta ccttcaacaa catccatttt attatttaat atttcattgt
    20341 atattccagc ttcaactttc aaatattctt cccagaattt tacttctgct tcttgacttt
    20401 catgattatc acttaaattt ctccattcag tatataaact catacttttt cctcctaagt
    20461 atacctatat ttttatagtt tttatagtat aaaatctttt agttagattg gaagtttctt
    20521 ctttttgatt ataccatatt ctatttaaag ttaactctta tattatatca taattaaaca
    20581 attcttttaa tacttttata gcatactctt ggtttataaa ataagggctt gtcgcaattg
    20641 ttattacttt ggctttttta attaagtttt ttatcttgta aaatttgagt tcaaatggta
    20701 tatagtccat atcacttgaa aatatatcca aatctatatc aagaacaaac tctgattcta
    20761 cttctaaatc aaatccatat agactatcta ttattattaa ttctgaaaat atgttatatt
    20821 ttaaagcagg ttttataaaa cttcccacat ttaaaacttc atttgtatat ctaaatacat
    20881 cttttagatt atttacatct acatcatagt tctcaggttc tctagtatcc ttatgttggt
    20941 ctacatgcac taacttgcat ccttttgtaa actcaccctt taacaaagat tttgcccaaa
    21001 aataaaatgc atggttatga ttgtcaaaca catatatctt tttatttccc aggacatatt
    21061 cgaccatatt ctccaatcct tttgctttta tttccttatt ttcatctact tcattaaata
    21121 ctacctcatt gcctaacctt acatcattta atgtaccatt tattagtttt ggaacgtata
    21181 tgctcttatt ttctctttta tcatatgaaa atacattatt tcctactggt ttttctatat
    21241 aaaatccact atattcataa ttttccataa aactttattt cctccattgt tatataaata
    21301 tataagttat aattttaaaa attaaaatgc acttattgta aatcatatca tctattatag
    21361 aaaacatctt atataactaa atataatatc ttctctcaat atgtattata caataagtgc
    21421 aaaatatatt tatataaaat atactacatt cctataacaa aaccatcagc ttcaattgga
    21481 aggtaactaa ctcttctatt caactccacc tttccaaaaa atactaccca atatggtcta
    21541 aaaatacttt caattttaac tatttcaatc tctgggattc ctcccataaa cctatgagtt
    21601 aatctcatag ccattttatt tgcctttgct tttactcttc gttcgtcctt atcactatac
    21661 tgcacaatat tacaatccac attttttaca acttcatatg taggaaaatc ttgtactata
    21721 ctaccagaac ctgtagaacc attcactaaa atttttaatt catttatatc attagaatat
    21781 ctcctattct tgaaaatgga tttcgttttg tgaattttaa gtgtgataat tttaaactct
    21841 atataatgaa gtctaacttc atgagcatat ttgttcttta atattagctt actaaataaa
    21901 gaattttttc cacttttagc tatctctata gcttcttctt ttgtaattct tataggtaac
    21961 gctcttatat ccataaaatc attctctttc ttattttttt cttgtcattt ttaaaactct
    22021 atatatataa tcaaaaaata ttacaattac aactgccata actccatatg gaattatttt
    22081 tccaactata atatcatttc tagtcaatat accataaaat ataaatcctg ttattaaaaa
    22141 gtacaataat actagaaaac cgtctattcc aagccatttt atttctaaat ctttttttac
    22201 cttagacact aatttagaca tagcagtttc tccttttata gattattttg tgtatttaaa
    22261 ttttcaccga ttctattacc aatcatccat gcaataaatg aaaaaggtat agctatcaca
    22321 agaggctcta actcaaagct ctgaactgga aatattttaa aataaccaaa agtcactcca
    22381 ccaacaatca ttcctaaaat accacccata gatgttcttc ttccatttcc aagaagccca
    22441 cctactatag gtacaaatgc cgaacccatc caaatacttc ctacaaatat acatgcatca
    22501 tatacatttt caaatctaaa tgctacgcta agccccataa ctgctgctaa acatacacct
    22561 attctagtaa actttactaa ttgtttctga gataactctt tttttgtaat tcttccatat
    22621 atatcatttg ctaatgtagc cccagctgct agataatagc tatccaatgt tgatattata
    22681 gaacctaaaa ttcctattat aaatattact cttattcctt gtggtaatgt atctaaaact
    22741 aaagttatat atccagcagc tggagccatt tctggataag tagctcttac tataagtcct
    22801 gtcatagtta acaccatgtc aaaacatatc catagtgaaa aacaggttaa taaggctctt
    22861 cttccaacct taggagaatc tgctgctgaa aatctttgat aaaaagttgg gtctgaataa
    22921 gctccaaaag aatatacaat taacatcaat gctttcatag ctgacatacc ttttgtaggg
    22981 tgcataagct gtggtgcatc tttgagtgct tctgtaagac cttgaaaccc tcctatttgt
    23041 tcaaattgac ctggcataac catagttaaa ccaaatacca tacaaaaaaa caataccata
    23101 tctgtaatgg caactcccat aagcccacca agtactgtaa gtacaacgac tactcctacc
    23161 attattaatc ctattaattc tttatctatt ttccatgcta cgttacctat agttcccata
    23221 gcagttattt ctccaagttg tgcacaatat ataaacatca aaattgcccc aattatagca
    23281 acatgcttgc cataaaaact ttccagtaaa tcaggaactg ttatattggt tcttatatga
    23341 atccttggac caacccacaa tgctaaaggc attctcccaa tatgtgcagc tattgaccaa
    23401 actgcccatg cagatattcc atatatagcc gcatactcta cactacctac tgtaccaact
    23461 cctccatacc atgatgcaac caaagttcct actattaagg accatggtaa acttcgattt
    23521 cctaggtaat aactttccat atcttttaca tttctagaaa aataataacc tattcctaat
    23581 aatataaatg caaatataac tataatccca ttttcccaaa atgttaacat atctaatctc
    23641 cacattctta tattttaatt tttaacttta atatctaaat atgaacaact ctacccattt
    23701 ccataaaaat aggtagagtt atataataat tataagtatt taagtaatct tgttattaag
    23761 caattttatt gtttaaacct ttattttctc ttttatttat tttatttaaa gcataagtag
    23821 caaatatccc tataactgca catactacag ataaaataaa tatatatgta tatcctgttt
    23881 gtccatattt atctaaccaa ttacctgcaa tagtgtaata aaacgcatct ggagaaaatc
    23941 ctataaatga agcaaatcca actactgctc ctgtagtttc taaagatata ttagactctg
    24001 aaacagatgc aaagtatata cctctaaatc caaataaaat tacacttagt attatcatgt
    24061 ttattacagc tatccatata aatcctgtac tcttaggaat aaccaagtaa gcagctgtac
    24121 ttactgtcat taatataaat cctacaaaca ttactcttat agatgaacca actttatctg
    24181 ttataacacc agcaactgga gatgctccca tttttataac ataagttctt attattgata
    24241 aagcagaaac tagagtcatt ggcattacat aaacttctga taagtatgga cttaaataag
    24301 ttaaacttga gaatactaaa taagttgaaa atattataag accaattaac caagcctttg
    24361 gtattgtaac tacttttaac aatgacttaa tacttaatcc ttcatctgat gctccactat
    24421 cttttttctc ttccactaag aaaaatagaa gtataccaca tattatagat gctgcagaat
    24481 ataaccatac tacatatttg aatcctactg ttatttcagc aaatttacca aagaaataaa
    24541 gacctataaa agaaattaat gtacctgata cacctgataa cccttcataa aaaccaaata
    24601 ttcttccttg ctctgactca cttccttgca ttctaacaac cttaacagag gctgagaaga
    24661 aggttaatat tgtcgtaaat ccccataata caaatattaa aagtatcata ctatatgatg
    24721 ggaatgtaga gaaataaaat ccaagtatcc ctgaagcaat taaagaaaaa gttattaatt
    24781 ttctaactga gaatctatct gcaaaccatc ctcctataaa ataagatacc attcctacta
    24841 atccatataa actcaataag ttccctattt gtgtatgact taatgctaaa gcttgttgca
    24901 ttgggtcata aaatgatgat ttcatatatg gtaaactata catagctgtt gttccaaatg
    24961 ccaatatgaa tattatcaaa aatcttttaa cattattttt gctttccatt gattctctct
    25021 ccttaaaatt ttattgttta taatctcatt catataattt tattttaatt tttctattgc
    25081 ttgtttaaaa tcatttatta tatcatttga agattctaat cctacagcta ttcttattag
    25141 attttcccca ttcatttgaa ctaatgtatg ctcttcccct aagcttgttg ctattgttgc
    25201 aagctctaaa gagttcatta atcttctact agcttggtct cttgtcaatc cactgatacc
    25261 atccttaagt ttaaatgaaa ctacgcctcc acctaaacca ttcatttgag ttttacaaag
    25321 ctcatattga ggatgtgatt ttaatgctgg atactttacc aattccacat tctcttcttc
    25381 ctctaaaaac tctgctactg ctaatccatt ttcacaatgc tttttcatcc tcatatctaa
    25441 cgttttcata ccccttaaaa tcatccatgc actcattggt ggtaaagatg ctcctgtaaa
    25501 acaacataaa cttggccatc taactaaatc tattaattct tttgaacctg ctactgctcc
    25561 ccctaaagta tctccatgtc catttataaa cttagtaaga ctatgtatta ctaaatctgc
    25621 acctaaatct attggtcttt gtacaattgg tgtcccaaaa gtactatcaa ctatagctaa
    25681 gcttttattt tcatgagcaa tttctgctat tgtttttata tctactaagt ctaatgatgg
    25741 atttaaagga ctttctatat aaactatttt agtatttggt ctcattgctt cttttacatt
    25801 ttgtggattt actgcatcta caaatgtaac ttcaactcca aatttagata acaactcaga
    25861 catgaataat ttagtgtgtg aaaatattcc atctgaacta actatatggt caccactttt
    25921 taccaatgct aatatagtag atgttattgc ccccatgcct gatgtactag atatggcact
    25981 ttctgcattt tcaagcatag ccaacttagc ttctaactct gtaagagtag gattacactc
    26041 tcttgtatac acatatcccc atgtcataca caactcttca aaatgctcta ctgtctttgc
    26101 agcaaatgtt gctgtttgac atattggtgt agccaatgca cctgttgttg ggtctgcact
    26161 atgtccccaa tgtattattt ttgtctctaa atcttgatta atattgttac tcattttgat
    26221 ttcctcccat tttgacttat ttatacaacg ttttccctgc caaatttcat caatttagct
    26281 tagtcttaat acattttttc aagtatactt ttttatttgt tataatttct aaaaatatga
    26341 cacttatttt gtttttttac agttctaatt ataaactcta tagtaactat agagtcaata
    26401 tgtttttgat atttttttta caatgtttaa aatagtcaat acaaaaatag gtatatctta
    26461 gaataactaa agtcatccta agatatacct atttttatat cacaatataa ataattacat
    26521 ataagcatta gtttttttat cataaataga tttttcaaga tttatcattt tttcataaat
    26581 atattatagt taaatatttt aatattacct aacatctaca ataaaatata ctcttaaaat
    26641 aaaattatat aattaaaaat actctataaa agctaaaaag tttttctatt cgctacatct
    26701 aaaaataatt agatagaggt ataaatgtat ttagtttatt tatacctcta gtcttttata
    26761 aattatatct ctactaaatt gattttttag cacgaataag tgtgctcttg gagataccag
    26821 tcatcttcac aacctctcta tatggcttag aatttaataa ccccaatgca tattgtatct
    26881 gttgattgct gtatttcttt ggcctaccat ctttaaagtc ctttttcttt ttagcaaatt
    26941 cttttcctaa ttgagtacgt tctattatca tatttctctc aagttctgca actgcaagta
    27001 atgtagtcaa aaagaattta cccattgaag tattttctaa aaggcctata tttaaaatat
    27061 gaacacttac ttctttagca aataaacctt ctattagttg tattccttct gatgtacttc
    27121 tagctagtct atccagctta gttgcaacaa gtaagtctcc cttttttaga gatgatataa
    27181 ttttttcaaa tttaggcctg tttgtagtag tgcctgtata agtttccttt attattttag
    27241 catcattata tttattcaaa atttcttgct gttgctgata caaactgttg ttgtctgttt
    27301 gattttttgt acttacacgg caatatccat atataataat catcacttcc taacataaag
    27361 attttgtaca gaagttaccc agaagcttgt ttttacttat ctttatccat tttactaaaa
    27421 cgatttttgg gtgtcaatac tgttaacttt tgacaccata ataattcctt tataatttgt
    27481 ttgactatgt caaaaattac caatataatg aaattaatga gtttatacaa attggaggaa
    27541 ataatatgaa tagtctgtta aaagaagcaa tagatgaatt aaaaaacatc cattgcgggg
    27601 aagttttttt attaaaagaa ttgttcaaag gctatgaatg gaacaggttg gagatgaata
    27661 tcagattgaa attaggaaaa ctttttttgt atgaagtggt aactaatccc aatttaaatg
    27721 tagaggtcat caataaaaat tctgcttatc agcaaatgta taaaaaaaaa tagtgactta
    27781 aatgtatata atagtataat cttcttattg attcaaaagt taaaactttt aatacaaaac
    27841 aatcattcta ttttaacatt tatcaatatg tctaaattta tataattaac acatacgaat
    27901 agtattgttt aaatcacatt atttaagata gataaatctt aaccctactc cagcataaat
    27961 actaaaatag ggcttagaat tatctatatt tttatcccat aaaatgctta gctcttcctc
    28021 ttgattgaac atatatatct ggggaaatac aatagaagcg taaatttata aaatttccaa
    28081 agtcagaaaa cttcagttca aacttaggta taccaaaact ttcaggtgct atttcgtcaa
    28141 cgccaaaata gatgataatt ccattatctg taagataaaa tgcttggtct tcaggtataa
    28201 ctaactcaac attagaataa tacatttctc tattttcatt tatttgagat ttaatgtaat
    28261 cagtaattat attaatataa tcaactccat ctctaaaaat gtcattaagt aaaagttctt
    28321 ttccagttgt caaatctata ttgtaattat tcaatgtatt gtatttaggc ccatagttat
    28381 tagcaaaccc cataaggttt aatatcatac tcagtacata atttttatta aaagtaactg
    28441 cgtaactggt agatacccaa tagctttgtt ttggaagtga attttgagaa gctgcgttat
    28501 tatattctcg ttcttcttcc tgtaagccat ttttaaaatt atttacagtc attcttacag
    28561 cattgtttat gttatttaaa ataactgggt ttacatacgt ggattgattt ctaatattgt
    28621 atataacagg atactttaca tcatatgaaa aatacttact tctttcaaaa tcttcattaa
    28681 attgtacata taatatatta tatgtacagg tatttgtaga ttgataattt tccattaaaa
    28741 tcctccccct tttataaaaa aatcagatac aatataatat gcttaaaaca gtaataatgt
    28801 taatggtaaa tgtaaagaat agaaagtatc taaaacagca aaaaatgact aattttgtgg
    28861 tataatacat aggattgaga gttattttat aggaggaaaa atatgttatt attagcagat
    28921 aaatggaaag attatgaact tatagatatg ggaaatggag aaaaattaga acgttgggga
    28981 agttatgtac ttagaagacc agacccacag gttgtatggc caatggaatc tgaatgggct
    29041 ttatggaaaa accctcatgg acattatcac agaagcaata aaggtggagg tcaatgggaa
    29101 cacaaaaaaa gatatccaga acaatggact ataaattata aaaacttaaa gtttcacatt
    29161 aaaccaactg gctttaaaca tactggatta ttcccagagc aagcagctaa ctgggattgg
    29221 atgatagaga aaataaagaa agctaacaga cctataaaag tgttgaatct ttttgcatat
    29281 acaggtggag ctactgtagc atgtgcatct gctggtgcag aagtatgtca tgttgatgct
    29341 gcaaaaggta tggttaattg ggcaaaagaa aatatagaat tatcaggtct taaaaatcaa
    29401 acagtaagat ttatagtgga tgatgttgta aagtttgttg aaagagaaat aagaagaggt
    29461 aaaaaatatg atgcaataat aatggaccct ccttcttatg gaagaggtcc taaaggagaa
    29521 gtatggcaaa tagaagaaaa gttatacagt tttgtagatt tatgtatggg agttttatca
    29581 gataaccctt tatttttctt aattaattct tatacaacag gattttctcc aatagttctt
    29641 gaaaatgtat tgtctacaac aataggtaaa aagacaaata caggagatat atacggtggg
    29701 gaattaggaa taccagcttc cagagatggt aagatacttc cttgtggtat atttggaaga
    29761 tgggagaaaa aataatgata aaagtcatat atgaagataa ccatttatta gttgtagaaa
    29821 aaccagttaa catattgtct caaggagata atactaatga taaagatatg gtaaatcttt
    29881 taaaagacta tattaaaaaa aagtataaca aacctggaaa tgtatatatt ggtttaatac
    29941 atagattgga tagaccagta ggtggagtta tggtgtttgc aaaaacatca aaagcagcat
    30001 ctagattgtc tgagcaggta agaagtaaga ctttcaaaaa aacttatcta gctgttgttc
    30061 atggcaagat aaaatctaac tcagatatac taaaagattt tttatataaa aataaaaaaa
    30121 ctaatatggt aactgtagtg aaaaaaagcc ataaagatgc caaagaagct gaacttaact
    30181 ataatgtatt agattttaaa gaaaatctaa gtttagttca agttaattta aagactggtc
    30241 gttctcatca aataagagtt caatttgcaa gcagaaaaca tccattatac ggagaccaaa
    30301 gatatggcaa agaaataaat aagattggac aacaaatatc tctttggtcc aataagatag
    30361 aaatttgcca tcctactaca aaagaaaaaa tggaatttac atgtcatcca cctgacaaat
    30421 atccatggaa tatattttaa atctcaaatg aggtgattat atgagtaaaa aattttcaat
    30481 aggagaaatg tctaaacttc ataaaataag catacagaca ctaaggtact atgaccaaat
    30541 agatttattt aaacctgttc atgttgacca agaaagcaat tacagatatt actcaattga
    30601 ccaattttca caattagata ttataaaata tttaaaatat ttaggtatgc ctttaaaaga
    30661 aattaagtca aagcttgatg aaaataatga agaaattcta aaattactaa caaataaaat
    30721 taatatagtg gatgaaaaaa ttaaagaatt agagcttata aagaaggtac taagcacaaa
    30781 gaaagaaact atggaaaaag gtatgaaagt agatgaagtt ggtaaaatag tgagaaaaca
    30841 tattccacga agaagtatct tatctataga gtatgaagaa ggattaagta gtgaagaaac
    30901 tatggaactt tccagaagac aaatagcaaa tatattagaa gataatatat ctgtgttcta
    30961 tggtggagta agtggacttg tatctataga taaattttta aaagaagata aagttattta
    31021 tgacaatagt tttgttactg ttgaaagaga cttatttgat ataaaatcac aaaaacatat
    31081 aacagaaatt tcatctggtg attttatttg tatgacatat aaagggcctt acaaagaaaa
    31141 ttataccgct tttaagaaac tggttgatta tgttgaatat aaaagaatac ctattgaaaa
    31201 ttatatatat gaaataccta taatagaccc tctgtctaca aacaacgaag aggaacttct
    31261 aacagaaatt caaatacaag ttaaaaagag agaaattgac ttaagatgtc tagtgtagta
    31321 atctagttta cttacacatc ttaatatagt tgtctattat caacatatga tttttctaga
    31381 tagtgattcc taacagtgag attttcaaaa aataggagga aatatggcta atgctaattc
    31441 cctcctattt ttaattaagc catatcattt taattaagcc atgtcatcac gaagtgcatc
    31501 aataatattt tcttttttta tcttattaat agtatacaac attgtaataa atactataaa
    31561 taaaacacta aatatactaa ttccaatact aaaccatgga atcacaaaat caatatcatc
    31621 tgtaaccatt actttatgaa ttagccatga agaaattact gctataggaa gtccaaagaa
    31681 caatgctctc atgccataaa aggcacactc aaaattcatc atcttttgga aatcatgctc
    31741 agacattcct acagagcgaa gcatggcaag ttctctacga cgtaacttaa tattagttga
    31801 aattgtatta aacacattgg caatcgaaat cagagaaatc ataattatga aagtataagc
    31861 aaaaacattg gcaataaaaa tgtaattacg gttctcatcc atcattccat acatattgta
    31921 cagattgtac tcattcgtaa ctccctcact ctcaataatt tgctccattt ctgcaactga
    31981 ctgagtagga tttttcgatt gaaagcttaa actttcaatt cctgtagaaa caccaggaat
    32041 ctcaaactta tctttggctg aataaggagc caatactctg aagcttgctt tcttcacatc
    32101 tgaacttctg attcctggag gagaatcagg tggcactact ttgacaaatg aaatattcac
    32161 attttgtctc tcaggtatct ccaacttgcc attagtttca ggagtgaggc taacattcat
    32221 agaagaactg ttgaaaatat caataatatt attatccttt gttaccatag aaacagcaat
    32281 cagttttgca ttttgtccag tatattcttc tgctggtaat ccaagacttt taataatatt
    32341 cagataagtt ctatcatcaa gaaattgcat atctactggt aattcaagca tttcactcat
    32401 agaatgtgaa ccctcatagt tcaaataatc gtctgtaagc tcacttactt tggcaacaca
    32461 tgaataggtc ataagtttct gatacaaacc ttcgtaaaca ccatttgcag tttttagctt
    32521 atcgtaaagc ttcaacattt cgttgtcttt cataccttgt gtagaaaaaa taatatcctg
    32581 gtcagtaacc tctgacctct gatttgacat ctgttttagg tctgctacta aagcacttgt
    32641 agatataaat agaactatgc ttaaaacaag cgacaataca atacttcgat aacgtttctt
    32701 gtttctttta aagtttttca tagcaagaac tccctcaaga ccataaactc gctgtgttag
    32761 ttttgatgtc ttcacagatt tagattcaat ttttacctca tttgtctgac gaatacactc
    32821 catcactggt gtgttggcag cctttcttgc tggtatgtaa gcagaaatta agattgtaac
    32881 catacttacc actgctgcaa cgatgattgc agggatagac aatgttaatg tcaacggaac
    32941 gtttacatag agaatatttg caaagttttt agatatgaca gaaatcacaa gtccaatact
    33001 gactataccg attgcaacac caattggtat accaatcgca ccaatgcaaa ttccttcaaa
    33061 tagcaccgaa tttcgtaact gttttgctgt agctccaaca gatgaaagta ttccaaattg
    33121 atgtgttcgc tcgttcaatg acatgttaaa tgaattatat attagaaaaa ttgagcctag
    33181 catgattatg gcaagcacaa tgccaccaac cgagtacaaa aacatattga acaaattgtt
    33241 atcagagaca cctatgaatc gcaacacatt gtcattgaaa atggaagcat ggtctttagt
    33301 gctacttgtg taagagcgaa tctgatgtgg atttttcaat gtgacaaaaa ggctaagact
    33361 gtcagtcttg acttgtttat cttctttggt tatcaaggta tatcctggtg cagaagattc
    33421 ttcaaatacg ggctttctac agatacccac aactgtataa gttttcttat cctgtggcac
    33481 aaaaacttca ctcccagcag tgtatgggtc actctgacca agcttttcat tttcactcat
    33541 tcgtttccct acatccaaag aaatagtatc ccctactgca aatttgacac caccatctgt
    33601 cgcaacttta cttgaaacaa gaatctctct actattttca ggtaatcttc cagaaattaa
    33661 agttataggc aaagtatcaa aagcttcttt gctaaatcca gcaataaaaa gatatggttt
    33721 atctggattt ctgttacctt caagtgttgc atagccaata ttttcaaatg ttgcagtatt
    33781 tgcaacttca tcattgactc tttgttcctg tacaaaagaa gaatccacat ccaaaaactg
    33841 gacatgccaa tcaccatatt ttataatagc tccatttatc atatagttca tcaaagaaac
    33901 tccaaaggta gtaactgctg taatcatagc agctgacaga acaactccta taattgttac
    33961 aagagttcga gttcgattct ttttcaatcc ttgcagagca actttgttaa aaatgttcat
    34021 taacgattca ccttcctctc atcacgcact acttttccat ctgaaataca gataattcta
    34081 tctgcctgta aagcaatgtt ttcatcatgt gtgacaataa tgagagtttg gtgatattta
    34141 ctatggctct ctttcagtaa ctggataatc tcttgtccat ttttactgtc taagctacct
    34201 gtaggctcat cagcaagcat aactgctggt gcattcatca atgcacgacc aatggcaaca
    34261 cgttgctgtt ggccaccaga aagttgattt ggtaaatgtg tttttcgttc ttttaaacca
    34321 agtaattcta acaagtcatt taaccgttct tgattgacct tacgcttatc cataagtata
    34381 ggcaatgtga tattttccac tacatttaat gtgggtatca gattgtgaaa ctgataaatc
    34441 aatccaactt gtcgcctacg aaaaatagca agtttttcat tgctttggcc atatacatct
    34501 tgcccctcca gatatatctt tccacttgtt ggtacgtcta ctcctgcaat ggaatgtagt
    34561 agtgtagatt taccagaacc agaagaacca atgattgcag taaactcccc tttttcaata
    34621 gtaagtgaga catggtcaag tgcagtaacc ttattttcat tcttaccata aaccttacat
    34681 aaatcttcaa tctttaaaaa cgccattata taagtctcct ttcatatttt ataaatatat
    34741 catagaactt tcaacttaca tttaagtgac atttaaatga gagattcgtc actttggaaa
    34801 acgaataaca aatattgcac caccttgtgg gtgattttgt gcagtaattg ttcctccttg
    34861 tctcataata atcgtcttac aaagtgccaa tcctattcca taaccggctg tgcttgaatt
    34921 tttcccacga taaaatctgt taaatagaca tggtaaatct tcttttttaa agcctgctcc
    34981 gttgtcatga atagtaagtt gagtaaacaa aaagttgtcc tcacaaatga tatcaatttt
    35041 tccattatca cccacactct ccatgcagtt tttaaatata ttttgaattg cttctgaaag
    35101 ccaatttaaa tcacccaaaa tccttactcc ttttggtata tcaatatgta aagtgatatt
    35161 gtgcagttcc atgggaatca aaagttgatg aagtgcagaa ctaatcaaat tattcacatc
    35221 aatttgttct ttttgaaaga ctacaatacc tgcatctaaa cgtgataatt ttaaaaggga
    35281 agtaatcagc cagtccattc gtataagtaa ttcctctgtt tctcgtatca actcttttct
    35341 ctcatcttca tcagaagtat tctctaacag tgacaaaata agagttactg atgtaagagg
    35401 agtgcgaagc tggtgggcta tgtcagctag tgagtcagca aggtgttctt tttctttttt
    35461 cagtgcgtaa ttttgctctc taatgcgtaa cgtcatttta gttatttcac tttgtagaat
    35521 agaaagctca ccctcatctg attcagcaat atacagatgg ttagcattat gaagcacaag
    35581 attaatttgt tctgaaatct gtgcaatact tttgtatcga tttttagtaa acacaaaaaa
    35641 tgctattcca aaggtagtgg cagaaacaat agaaagtatt ccagtcataa tactgattgc
    35701 aaatcccagg gctacagtga gaatagtaat taataagaat aaaatagcaa attttcgaaa
    35761 ctctctattt cgaaacataa cttcccccca atctataccc tgtcccacga acagtcaaaa
    35821 ttatttgtgg acttgaaggg tcatcttcta tcttctctcg caagcgtttg atgtacacag
    35881 tcaatgtgtt gtcattaaca aactcacctg ccgcatccca caattcatca agtaatctta
    35941 ccctcgtaat aatactttta ggattgctaa taaacaccaa taacaaacgg tattctaaag
    36001 ctgaaagaaa aacctcattg ccattctttt tcacaacacc agttgctgta tctacataaa
    36061 gcccacaaat ttcaaaagtt gacccaaaac gcccactttt tcgtagtgct gttctaattc
    36121 gcgcaatcag ttcacgtgga cgaaaaggtt tagtaatata atcatctgca cccatattca
    36181 gtccagtaac aacacttgat tcatcaccgg aagctgtcaa aaagataaca ggaatgtctt
    36241 gcttttcttt gatttctgtg caaatcgtaa acccatttcc atcaggtaaa gaaatatcaa
    36301 taagtgccaa atcaaattta ttcttagcaa tctctacaag tgcttcactt cttgtggata
    36361 catgggttac tataaatcct tctgaattaa gcaaaagaat aagatttttg gcaattgctt
    36421 tatcatcctc aactaaaaat atccgtttca tttactttca tcctccttta ttttattagt
    36481 tttatatatt ccagcagagc aatagtacac tactatttta cttatcatta cacaattttc
    36541 agtagtttct tttgttctta tataatccat gcataatacc tccattgatt tattatattt
    36601 cttataacat aaatacacaa gataagacaa aaaacataat gtaagtgcaa gttttttaac
    36661 tcactaactt ataaacatac actcctacta acactacttt cttgatgaca catcttacaa
    36721 gcttatattc atacatcaat gaaaaattaa atttatccat ttattaaaat aaaaggcaat
    36781 agcttctata gctaatgccc tttatcttaa aatgatttaa atactataca atttttataa
    36841 atggctataa ataaactcgt atagaatata aaatttatct acagtctttt gttatacaaa
    36901 atttataacc tcttatagaa caaatttacc actttgcaaa cttatcactt ttactcagct
    36961 atattataaa cttttaattt atctttgttt aaatgtttct tcttaacttc tactaaagct
    37021 tttagcgtat ccaaagatgt cattatttca gtagaaaatt caatggctgt tcttctcatc
    37081 ttaaatccat ctcttgtaga gtcatttcct ttagttggtg tatttattac tatatcaact
    37141 tgtttatttc ttatagcatc taatatgttt ggtctaggtt cttcaattct atttactata
    37201 tcagcctcta ttccattttc tcttaaactc ttcgctgttc cttctgtagc aacaaaagta
    37261 tatcctaatt ctttcatatc ttttgctatc tctataaact catctttatc atgattattt
    37321 acagtagcaa gtactactcc tctttcatca gacatatgtc tacctgctgc caagaaccct
    37381 ttatataagg cttcttctaa attttctcct acacctagga cttctcctgt agacttcatt
    37441 tcaggtccta gacttacttc aactttagat aatttagaca ttgagaatac tggtactttt
    37501 actgatacta attttggctc tttgtaaact cctgtaccat atcctaaatc ctttaattta
    37561 gctcctaaca tacacttagt agccaagtca actattggta ctccacttac tttacttata
    37621 tatggaactg ttctactagc tctagggtta acctcaatta tatataattc attttgaaat
    37681 tctataaact gtatattaac cataccaagc acattaaggt ctaaagctat ttttttagtg
    37741 tattctacta tcttttcttt tatttcatca gatatatttt gacttggata cattgttgta
    37801 ctatctccag agtgaactcc agctctttct aaatgctcca ttataccagg tatcaatata
    37861 tcttctttat cacatatagc atctacttct atttctctac cagttagata tttatctata
    37921 agtactggat ttttatggtc tctatcaaat gcatcatcca aatactgaat tagtttttct
    37981 tcgttataag ttatttccat accttgacca ccaagtacat aagatggtct aactaatact
    38041 ggataaccta actccttagc aatttcaaca ccttcattag ttgtccatac accttttcct
    38101 tttggtctat ttatatctag tctctctagt aaatcatcaa atttttctct atcttctgct
    38161 gcatctatat ctctaaagtc tgttcccagt ataggtatat ttttttcatg taaaaactta
    38221 gccaatttta tagctgtttg tccaccaaac tgtagtatta caccttctgg tttttctttt
    38281 tctattatat ttaaaacttc ttcttctgtt aatggttcaa aatacaactt atcagatgta
    38341 tcaaaatccg tacttacagt ttcagggtta ttgtttacaa ttatagtttc tattcccatt
    38401 tttcttaaag attttacaca gtgaactgaa caatagtcaa attctattcc ttgacctatt
    38461 cttattggac cagaacccaa tactacaact tttctcttat cactaactac tacctcatca
    38521 tattgttcat aagttgaata gtaataaggt gatagagctt caaattctcc tccacatgta
    38581 tcaaccattt tataaactgg ttgtatatta tataaacttc tcaattcata taatttttct
    38641 gggcttatct tcattaagtc agatatacct ttatcagaaa atcccttttt ctttaattca
    38701 tgtaaatatt ctttatctaa gtcttctatt tttagaactt ttaacttttc ttcttgttct
    38761 actatccatt taaatttatt tataaaccac ttatcaacac cagttatttc tgatatcatt
    38821 tctactttat agcctcttct tatcatttca gctaagtcaa atagtctctc atcatcaggt
    38881 actactactc tagcctttaa ttcttctaat gttctacctt ctgatggagc atgaaccaat
    38941 gaatattttc ctgtttcaag tgatcttata cctttaagaa tagcagcctc aaagttactt
    39001 cctatactca tgatttcacc agttgccatc atcttagttc ctaattttct attggcatgt
    39061 ctaaatttat caaatggcca ctttggtatt tttactacca cataatctaa tgttggctca
    39121 aaacaagcat aagttttttt agttactgca ttttctatct catccaaagt ataacctaaa
    39181 gctatcttag ctgctacttt tgctattggg tatcctgtag cttttgatgc tagggctgat
    39241 gacctactaa ctcttgggtt tatttctatt atcgcatatt ctaaactatg tgggtttaaa
    39301 gctatttgaa cgttacatcc accttgaacc tctattgcat ttattatgtc tattgatgcc
    39361 tttctaagta gttgatactc ttcatcactt agagtttgac ttggagcaac aactatactg
    39421 tctcccgtat gaactccaac tgggtctaca ttttccatat tacatacagt tatacagttt
    39481 ccatttccat ctctcattac ttcatattct atttctttcc aaccttttat actcttttca
    39541 agaagaactt gtccaacagg acttagctgt agtccatgtg aaagtatttc tcttaattct
    39601 tcctcagtat cagctatacc tccaccagtt ccacctagcg tatatgctgg tcttacaact
    39661 actggatatc ctatcttatt ggcaaactca agacctgctt ctaaatttgt aactatatca
    39721 ctaactatta ctggttgatt tatttctttc attacttctc taaataaatc tctatcttcg
    39781 ccctttttta tagattctat agatgttcct ataactttta cattatattt atctaatata
    39841 ccagcatcat gtaattctac agctaagttt aatcctgtct gaccacccat acctgcaagt
    39901 aaactgtctg gtctttcttt ttctattatt ttttctataa attctattgt taatggttct
    39961 atatatattt tatctgcaat ttctttatca gtcattatag tagctgggtt actatttact
    40021 aatacaactt caattccctc ttcttttaat gcttggcagg cctgtgttcc tgaatagtca
    40081 aactctgcag cttgacctat tattattggc cctgacccta atactaaagt tttctttata
    40141 ctatctaatt taggcataac tctttctcct tatctaaaca attttcacag ttcttatctt
    40201 catcatctat acaatcaact ttaattactt tattttatct taagttcaaa tttactcatc
    40261 aatacatcta tttaatacat ctatgttaat taaaccaaac tttttgattt aagttattct
    40321 tgttttttag ttagctattc taatgttgtt tattttacta attctaaaaa tttatcaaaa
    40381 atatagtcat tatcttttgg tcctgggcaa gcttcaggat ggtattgaac actgtataca
    40441 tctagcttct catgtcgcat tccttcaaca gtattatcat ttaaatttat atgtgtcact
    40501 ttcatttgtt caggaacttc tgaaacataa taaccatgat tttgagaagt tataaatact
    40561 tttccttctt ctaaatcttt aactggatga tttccacctc tatgcccaaa tttaagttta
    40621 gcagtttttc ctccaagagc taaagctaaa agttgatgtc ctaagcatat tccaactata
    40681 ggttttttac caataagagc ttttatattt tctataacat cttctaagtc ttctgggtct
    40741 ccaggtccat ttgacaagaa tattaagtca gggtttatac tcaatacatc ttctggagat
    40801 gttgtagctg gaaatattgt tatatcacat ccacgagcta taaatgatct taatatattt
    40861 ctttttactc caaaatccat aacagccact tttggaccat ttccttttat atgctctact
    40921 tcttttcttg taacagttct aactgcttct gtatttgaaa acttatccaa tttatgttta
    40981 acatcttcta atttactgtc ttctaatgta attatacctt tcattgttcc attatttcta
    41041 agtatcttag tcaaagctct tgtatctatt ccttcaagtc cgataacttt attttgcttt
    41101 aaatattggt ctatatcaat ttcacatcta aaattacttg gagaatcact tttctccctt
    41161 actatgaatc cctttacttg aactttttca gactctgctg atgataaatt tataccataa
    41221 tttcctacaa gaggataagt catagtaact atttgaccat agtaagaagg gtctgttaaa
    41281 acttcaccat atcctatcat cgaagtatta aatactactt ctcctacact ttcttctaaa
    41341 tatccaaatg cctttcctat aaaaactgtt ccatcttcta atattaacct tgctttcata
    41401 ctacagtacc tcctataatt ctctttagac aaactaaaga acattaacta aatataagct
    41461 tttaaatatc tatatctatt tttcactttt acattctagt tttatactta tttacattta
    41521 ttttttatct ttttctatta tactacaaat ttcttaaaaa aaagaagtta tctcaaaagt
    41581 aaaaactcct agaaaaaata acctcttttt tttatttaat aattaaattc ttaaactata
    41641 caatcaactt aatttctatt cagctatatt gtaaactttt aactcatctt tatttaaatg
    41701 tttcttctta acttctacta aagcttttag tgtatccaaa gatgtcatta tttcagtaga
    41761 aaattcaata gctgttcttc ttatcttaaa tccatctctt gtagagtcat ttcctttagt
    41821 tggtgtattt attactatat caacttgttt atttcttata gcatctaata tgtttggtct
    41881 agattcttct actctattta ctatatcagc ctctattcca ttttctctta agctattagc
    41941 tgttccttct gtagcaacaa aagtatatcc taattctttc atatcttttg ctatctctat
    42001 aaactcatct ttatcatgat tatttacagt agcaagtact actcctcttt catcagacat
    42061 atgtctacct gctgccaaga accctttata taaggcttct tctaaatttt ctcctacacc
    42121 tagaacttct cctgtagact tcatttcagg tcctagactt acttcaactt tagataattt
    42181 agacattgag aatactggta cttttactga tactaatttt ggctctttgt aaactcctgt
    42241 accatatcct aaatccttta atttagcccc taacatacac ttagtagcca agtcaactat
    42301 tggtactcca cttactttac ttatatacgg aactgttcta ctagctctag ggttaacctc
    42361 aattatatat aattcatttt gaaattctat aaactgtata ttaaccatac caagcacatt
    42421 aaggtctaaa gctatttttt tagtgtattc tactatcttt tcttttattt catcagatat
    42481 attttgactt ggatacattg ttgtactatc tccagagtga actccagctc tttctaaatg
    42541 ttccattata ccaggtatca atatatcttc cttatcacat atagcatcta cttctatttc
    42601 tctaccagtt agatatttat ctataagtac tggattttta tggtctctat caaatgcatc
    42661 ttgtaggtat tgagataatt tttcttcgtt ataagttatt tccatacctt gaccaccaag
    42721 tacataagat ggtctaacta atactggata acctaactca ttagcaactt caataccttc
    42781 gttaagtgac catacaccct ttccttttgg tctatttata tctagtctct ctagtaaatc
    42841 atcaaatttt tctctatctt ctgctgcatc tatatcatca aaatcagttc ccaatatagg
    42901 tatatttttt tcatgtaaaa atttagccaa ttttatagct gtttgtccac caaactgtag
    42961 tattacacct tctggctttt ctttttctat tatacttaaa acttcttctt ctgttaatgg
    43021 ttcaaaatac agcttatctg atgtatcaaa gtctgtactt acagtttcag ggttattgtt
    43081 tatgattata gtttctatgt ccatttttct taaagatttt acacagtgaa ctgaacaata
    43141 gtcaaattct ataccttgac ctattcttat tggaccagaa cctaatacta tcaccttttt
    43201 cttatcacta acaactacct catcatattg ctcataagtt gaataatagt aaggagatat
    43261 agcatctatc tcacctgcac aagtatcaac cattttatat gctggtttta tattgtatag
    43321 acttctcaat tcatataatt tttctggact tatcttcatt aggtcagata tacctttatc
    43381 agagaatccc tttttcttta attcaagaag atagtcttta gttaaatctt caattttcat
    43441 aacttttaat ttttcttctt gctctactat ccatttaaat ttatttataa accatttatc
    43501 tatacctgta atttgttcta tcatttctac tttatagcct cttcttatca tttcagctaa
    43561 atcaaatagt ctctcatcat caggtactac aactcttttc tttaattcct caatgcttct
    43621 ctcctcagat ggtgtgtgaa ctaatgaata ttttcctgtt tcaagtgatc ttatcccttt
    43681 aagaatagca gcctcaaagt tacttcctat actcataatt tcaccagttg ccatcatctt
    43741 agttcctaat tttctatttg cttttttaaa tttatcaaaa ggccattttg gtatttttgc
    43801 caccacataa tctatagttg gttcaaaaca agcataagtt tttttagtta ctgcattttc
    43861 tatctcatct aaagtataac ctaaagctat ttttgacgaa actttagcta ttggatatcc
    43921 tgtagctttt gatgctaaag ctgatgacct actaactctt ggatttatct ctataactgc
    43981 atattcaaaa ctatgtggat taagtgcaaa ttgaacgtta caaccacctt gaacttctac
    44041 tgcatttaat atatctaaag atgctttttt aagcattgca cattctttgt tacttaaagt
    44101 ttgagttgga gcaacaacta tactgtctcc tgtatgaact ccgactgggt ctatattttc
    44161 catgttacat acaactatac aatttccctt tgaatctctt attacctcat attctatttc
    44221 tttccaacct tttatactct tttcaattaa tacttgtcca acaggactta attgtagacc
    44281 atgtgaaagt atttctctta attcttcctc attatcagct atacccccac cagttccacc
    44341 tagtgtgtat gctggtctta caactactgg ataaccaatt gtaagtgcat aatcaagacc
    44401 tgcttgtaaa tctgtaacta tatcactaac tattacaggc tgatttattt ctttcattac
    44461 ttctctaaat atatctctat cttcaccttt tttgatagat tctactgaag tacctattat
    44521 ctttactcca tatttatcta gtatgccttt ttcataaagt tcaactgcta ggtttagccc
    44581 tgtttgtcca cccatacctg caagtaaact gtctggtctt tctttttcta ttattttttc
    44641 tataaattct attgttaatg gttctatata tattttatct gccacttctt tatcagtcat
    44701 tatagtggct ggattactat ttattaatac aacttcaatt ccttcctctt tcaaagactg
    44761 gcaagcttgt gttcctgaat agtcaaactc tgcagcttga cctattatta ttggtcctga
    44821 ccctaatact aaagttttct ttatactatc taatttaggc ataaattttt ctccttatct
    44881 aaatggtttt tactatctgt aaataatact aaatctatat taatgctaag aatttattaa
    44941 atatacaatc taaatcttct tttgttgtgt attctggaat gtattgaaca ctatatattg
    45001 gtaattcctt atgtctcatt ccttctatag tattgtcatt taaatttatg tgagttactt
    45061 ctgtattttc tggcatttct gaaacattat atccatgatt ttgagaagtt ataaatacct
    45121 ttccagtttc taaacattta actgggtgat tacctcctct atgtccaaat acaagctttg
    45181 aagtctttcc tcctaatgcc aatgctaaaa tttgatgtcc taaacctatt cctgtcaatg
    45241 gctttttgcc tattaatttt tttatatttt caactatatc atttatatcc tctggatttc
    45301 ctgggccatt agataataag attaagtcag gatttatatt taaaatttct tctactgtag
    45361 tcattgctgg tactatagtt atatcacagt tacaagcctt aagagactct agtacactat
    45421 ttttaacacc aaagtctata actacaactt tagctccagt accttttata ttttcaattt
    45481 cttttcttgt aacatttttt actgaatctt tgttacaatg ctcttctaat ttagactgaa
    45541 catcttgtaa tgatacattt tctaaagtta taattcctct cttagttcca ttgtttctaa
    45601 taattttagt taatgctctt gtatctattc cttcaagtcc tattacctta ttttgcttta
    45661 gatatgtgtc tatatccatt tcacatctaa aattatttgg gttatcactt ttttctctta
    45721 caataagtcc ttttacttgc accttatttg attcaacatc ttctaaattt attccatagt
    45781 ttcctataag tggataagtc atagtaacta tttgtccata ataagttgca tctgtcaata
    45841 gttctccata cccagccatt gaagtattaa atacaacttc tccaatagct tcctttagat
    45901 atccaaatgc ttttccttca aatactgttc catcttctaa aattaatttc cccttcatgt
    45961 aagctacctc ccaaatattt tatctaagca ttttctattt ctcttacaat attaatagct
    46021 gattctcttg gattttctgc ttttgttata ggtcttccaa caactaaata atgagcacct
    46081 ttttttattg catctgatgg agttacaact cttttttggt ctccaacctc tgctgattta
    46141 ggtcttattc ctgggcaaac tgttataaaa tcagttccac atgcttctac tattttatct
    46201 acttcttgcg ctgaacaaac aaccccatct attcctgatt cttttgccag tttagctctc
    46261 tttatagcta actcttctgt tgtcatatta catcctatgt cctgtaaatc ttggttgctt
    46321 aatgaagtta aaactgttac tccaactact aatggctttt ctatatttaa tctttctgct
    46381 tcttccctag caattagtgc acattgtctc attccttcca tatctgatac atgtattgtc
    46441 attaaccaaa cattatctct cactgcagct ttcactgcac ttttcatagt atttggtata
    46501 tcatggaatt taaggtctaa aaatattttt ttcccttttt ctttaagata atctattgtt
    46561 ttaccttttg tggcaacgta ttgctctagc cctaccttaa atatgtctac actatcttct
    46621 aatctgtcta ttagttcttt agctttatca aattcgtcag tatcaattgc aactatcaat
    46681 ttttctttac catttatcat ttttacatct cctttttaat aaagcctaaa taatcataaa
    46741 aaaatcctca caccaaaaga ggcataagga ttttatctat acataactct attcattagc
    46801 tatgagatat ctttcccagt ctctctggac tagtttaaaa gactttatta tatttaaatt
    46861 tttattttat actcatcaaa aacctctaga agatttacta gaggttatat actaacatta
    46921 tctataaccc tttagtctct ctggactact ttaaggaatt tagctgtaat atttttcttt
    46981 atttatataa ctttatgtat ctataggata atttaaagtt ttatcttttt tataattaaa
    47041 ccactaattg ctttttatgt caataagtct ttgaattatt cttcatcttt atcaaacaat
    47101 tttttctcaa aatatgattc tgcattatat attgcagcaa ctggattatg cccagttact
    47161 ctatctttga caactaaagt tgttatagga gcttccatat atttcatggc taaagtatcg
    47221 tgaccaacac ataatccaag aattacatta aggtcaacct tagcttcatt taaaaataaa
    47281 gcttgtccta ttggattgca cattacctca tctgaacact ttgatattgt ttgagatgct
    47341 tctagtccaa ttgtactctt aggcattgcc ccatttttac atataacaga aaatacttca
    47401 aacccatgat gttcaaatat tttttgcact gtttgcattt ctctttttag tccaatacaa
    47461 aatgctatgc caatcttttt ataaccacac tttttagcaa actctataat ctcttttagt
    47521 ctagtatatt gacaataccc ctctgcttca actaatccag cattataagc tatttttcta
    47581 ttctcttctt cttcataaag ttgctttgct ttttgttgta gttcaatttc tctagttgga
    47641 catatcttca ttaatttatc catatttcca ttagaacagc ctctaagatt acattttcca
    47701 cacttataca tatccaatat ccccttccaa aattttattc atcttgcgat tcttcttttt
    47761 ctagagttac attttcttct tgggtttctt cttctggttt cacatgctcg actacagtta
    47821 taaaatatga ttttcctatt ttattcctta taggaagttt ttcatatgaa acatctattt
    47881 tattttttat acaagctata tcaaaatgtc ttaaggctac acctaagcta accttattca
    47941 tatcttcaca gtgtttctta ggtctctttt caaataaatg aagacctcgt tcatccttat
    48001 ggaaatacca cggttgagaa ttttttacag atggagctct tcttgtcaat tctaagatat
    48061 ctttccactt tctattccta tttatcttat ttatcatatc ttttactggc tttctatcag
    48121 gtctagcata tctcgtccta aacaactctt cattttttgc tggatatcca aatgctatta
    48181 ttatataagg attttctatt ttgttttcat catcttcttc tgtttttatt ccagctagat
    48241 aatctaaatc atcttcctca tcttcttcaa aattcttatt cccaatatct acaaattcca
    48301 agatatcttc tctctttaag tttccttcca accagcaagt agctattccc aatgttgtaa
    48361 ggcgcaaaac tacaccttct gtagcaaatc ctatattctg taaatagtcc tcacctttgt
    48421 ctgatgtaac tactatataa tgaggtgcct ttactttaca ttccttcccc atcaaaaaat
    48481 gtattatatg acctctatca acaacatgtg ctttaatatt taaatcttca tttaagtatt
    48541 ctaaattttt acaacttctt tttatttctt ccatcaatgg cttttttatc tttttactgg
    48601 aaaaatttct tacagatttt ctataaaaaa tggcttcata aagctccatt tatgccctcc
    48661 taactgaaat cttctgacgt ataaactcta taaatttata taaatacttt atatttttta
    48721 tagttttatt tcctttttcc tcctgcccta ttttgaaatt cataagaata tctactctag
    48781 tttaatatag cattcaaaag gcttaaattc taccttatca atgtctatac attagcataa
    48841 tttttttatt tgattttcta tgctaattta aataacaaca ctagctatta cctttttaat
    48901 taagctttgc cagaaaaatt ttttttcatt tataaatatc tatctcatta ttatattata
    48961 agtttttttc ggtattcttg tatagctttt cactaaaaaa gtatttttat ttctacttta
    49021 atattattat caacaaaaaa catactactt aaataagttt gttatcctca taatttgttt
    49081 taattaaaat atttttttta ttt
//
LOCUS       DAYATN010000016        44817 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.016, whole genome shotgun sequence.
ACCESSION   DAYATN010000016 DAYATN010000000
VERSION     DAYATN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44817
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.016"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(183..431)
                     /locus_tag="ACGI8A_000901"
     CDS             complement(183..431)
                     /locus_tag="ACGI8A_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0178909.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVKDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(462..923)
                     /locus_tag="ACGI8A_000902"
     CDS             complement(462..923)
                     /locus_tag="ACGI8A_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0178910.1"
                     /translation="MKFGGVLLIVKDINKSKKFFEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQV"
     gene            complement(961..1794)
                     /locus_tag="ACGI8A_000903"
     CDS             complement(961..1794)
                     /locus_tag="ACGI8A_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0178911.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSWMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(2068..2754)
                     /locus_tag="ACGI8A_000904"
     CDS             complement(2068..2754)
                     /locus_tag="ACGI8A_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178912.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(2769..5246)
                     /locus_tag="ACGI8A_000905"
     CDS             complement(2769..5246)
                     /locus_tag="ACGI8A_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0178913.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGNMNEEFIEDVRHMEGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGVTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(5465..6817)
                     /locus_tag="ACGI8A_000906"
     CDS             complement(5465..6817)
                     /locus_tag="ACGI8A_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0178914.1"
                     /translation="MIFICLILTVIGIFLSLITIQKPNGEFIRSDWPKKFTENFNEQI
                     VFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPKEHKDYYSNSELLALANQ
                     NNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGKFIGGKSIVFSIIGIIFL
                     IILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVFADIYNSLNILNEEIHKS
                     DEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSSTNYSPEKVYKYASIMLKN
                     IEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSIDILNYPEYESNIVEFKSN
                     IDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLNERIEIIISDNGKGMSRE
                     EASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNISVESIPGLGSSFYITFPI
                     "
     gene            7367..8227
                     /locus_tag="ACGI8A_000907"
     CDS             7367..8227
                     /locus_tag="ACGI8A_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0178915.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(8466..9023)
                     /locus_tag="ACGI8A_000908"
     CDS             complement(8466..9023)
                     /locus_tag="ACGI8A_000908"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0178916.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(9213..10100)
                     /locus_tag="ACGI8A_000909"
     CDS             complement(9213..10100)
                     /locus_tag="ACGI8A_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0178917.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            10219..11418
                     /locus_tag="ACGI8A_000910"
     CDS             10219..11418
                     /locus_tag="ACGI8A_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HFH0178918.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(11612..14167)
                     /locus_tag="ACGI8A_000911"
     CDS             complement(11612..14167)
                     /locus_tag="ACGI8A_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0178919.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(14164..14850)
                     /locus_tag="ACGI8A_000912"
     CDS             complement(14164..14850)
                     /locus_tag="ACGI8A_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178920.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            complement(14927..15835)
                     /locus_tag="ACGI8A_000913"
     CDS             complement(14927..15835)
                     /locus_tag="ACGI8A_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0178921.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(15837..16508)
                     /locus_tag="ACGI8A_000914"
     CDS             complement(15837..16508)
                     /locus_tag="ACGI8A_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0178922.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            16770..17834
                     /locus_tag="ACGI8A_000915"
     CDS             16770..17834
                     /locus_tag="ACGI8A_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HFH0178923.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            17992..18678
                     /locus_tag="ACGI8A_000916"
     CDS             17992..18678
                     /locus_tag="ACGI8A_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0178924.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            18668..19699
                     /locus_tag="ACGI8A_000917"
     CDS             18668..19699
                     /locus_tag="ACGI8A_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0178925.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSRICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            19781..20446
                     /locus_tag="ACGI8A_000918"
     CDS             19781..20446
                     /locus_tag="ACGI8A_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178926.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            20459..22876
                     /locus_tag="ACGI8A_000919"
     CDS             20459..22876
                     /locus_tag="ACGI8A_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0178927.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(23057..23848)
                     /locus_tag="ACGI8A_000920"
     CDS             complement(23057..23848)
                     /locus_tag="ACGI8A_000920"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0178928.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(23881..24468)
                     /locus_tag="ACGI8A_000921"
     CDS             complement(23881..24468)
                     /locus_tag="ACGI8A_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0178929.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            complement(24645..26036)
                     /locus_tag="ACGI8A_000922"
     CDS             complement(24645..26036)
                     /locus_tag="ACGI8A_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFH0178930.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(26220..27524)
                     /locus_tag="ACGI8A_000923"
     CDS             complement(26220..27524)
                     /locus_tag="ACGI8A_000923"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/bile acid-CoA:amino acid
                     N-acyltransferase family protein"
                     /protein_id="HFH0178931.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            complement(27883..28758)
                     /locus_tag="ACGI8A_000924"
     CDS             complement(27883..28758)
                     /locus_tag="ACGI8A_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HFH0178932.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDINTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(28962..29648)
                     /locus_tag="ACGI8A_000925"
     CDS             complement(28962..29648)
                     /locus_tag="ACGI8A_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFH0178933.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(29697..30230)
                     /locus_tag="ACGI8A_000926"
     CDS             complement(29697..30230)
                     /locus_tag="ACGI8A_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFH0178934.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(30274..30906)
                     /locus_tag="ACGI8A_000927"
     CDS             complement(30274..30906)
                     /locus_tag="ACGI8A_000927"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HFH0178935.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(30996..32075)
                     /locus_tag="ACGI8A_000928"
     CDS             complement(30996..32075)
                     /locus_tag="ACGI8A_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFH0178936.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(32208..33554)
                     /gene="pepV"
                     /locus_tag="ACGI8A_000929"
     CDS             complement(32208..33554)
                     /gene="pepV"
                     /locus_tag="ACGI8A_000929"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFH0178937.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELNKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(33623..34903)
                     /locus_tag="ACGI8A_000930"
     CDS             complement(33623..34903)
                     /locus_tag="ACGI8A_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0178938.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(35030..35290)
                     /gene="hfq"
                     /locus_tag="ACGI8A_000931"
     CDS             complement(35030..35290)
                     /gene="hfq"
                     /locus_tag="ACGI8A_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFH0178939.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(35404..36339)
                     /gene="miaA"
                     /locus_tag="ACGI8A_000932"
     CDS             complement(35404..36339)
                     /gene="miaA"
                     /locus_tag="ACGI8A_000932"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFH0178940.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(36377..38344)
                     /gene="mutL"
                     /locus_tag="ACGI8A_000933"
     CDS             complement(36377..38344)
                     /gene="mutL"
                     /locus_tag="ACGI8A_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFH0178941.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(38355..41198)
                     /gene="mutS"
                     /locus_tag="ACGI8A_000934"
     CDS             complement(38355..41198)
                     /gene="mutS"
                     /locus_tag="ACGI8A_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFH0178942.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIENLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALIKQNDDKEKINQVDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(41358..42092)
                     /locus_tag="ACGI8A_000935"
     CDS             complement(41358..42092)
                     /locus_tag="ACGI8A_000935"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178943.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(42061..43074)
                     /locus_tag="ACGI8A_000936"
     CDS             complement(42061..43074)
                     /locus_tag="ACGI8A_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0178944.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(43096..43866)
                     /locus_tag="ACGI8A_000937"
     CDS             complement(43096..43866)
                     /locus_tag="ACGI8A_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0178945.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(43841..44131)
                     /locus_tag="ACGI8A_000938"
     CDS             complement(43841..44131)
                     /locus_tag="ACGI8A_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HFH0178946.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(44262..44361)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT        15258 a         7010 c         5114 g        17435 t
ORIGIN      
        1 caaaatttat atactttaaa atatctaaga aattattcaa taaaaaaata ctatttttta
       61 aaaattatca ttattgataa agctctacta tttatatttt ttagtgtatt catgtataat
      121 aagtaaaagc acaacggtat atactgttgt gcttttttgt aattaataaa caatattttg
      181 gtctataaca atgaaaaatg gctactttcc ataattgatt tcatctgaca taagcaaaaa
      241 gggtgtccag ctgggtcaat cataactctc cagtcatcag taaattgctc ctctgcgatt
      301 gtagctccac aatgaattgc atattgaact gcttcttcta aatccttaac agcgaaatcc
      361 atatgagcca tttgctgttg agattcaggt ttttctggtt ctattatgcc ccaagccttt
      421 gttttttgca atataaagaa aaaattatat attttaatat tttatacttg ttgaaattgt
      481 gtaataaatt caactggata catagtgcgt tttgaaattt cttctaaagc taaaccttgt
      541 tttaaaagcc tctttatgac gctttccata ctttctgaaa tttctataat atttttatca
      601 aaatcataaa acctaaaagt tctttgaccc catgggtatt cttttatata atgcaaaaat
      661 tcaatatttc ctattctatt tatcttactt tcccattgtt tgatgtcatt aacctcaaaa
      721 tacaactgaa agttatttga ttgttgatat attggtaaat catatccaat tatagcttta
      781 taattacttt gtaatccaaa tccattttca aatgatacat gttctcctaa atctaatgtg
      841 attttttgct ccataacatc ttcaaaaaac ttttttgatt tatttatgtc tttgacaatt
      901 agcaatactc ctccaaattt catcttatca ctatcctttc tttgttataa gtattataac
      961 ttaaaatttt gcaatttcac tgtaaaaatc agacatatta gctcggtatt tttgaggagt
     1021 tattccacag acaattttaa aatctttaat aaaatgtggt atatcataga atcctaattc
     1081 attacatata gacatccatg aagttttatt attgtttaga agttttatag atttattaat
     1141 acgtaccaaa cgagaaaatg acttcatact caaccctaga tactgattaa agagtctgtt
     1201 taaatgccta gaactataaa atactttttt agaaatttct tgtgaagata tatttccaga
     1261 gttttgaata atcaactgaa ttgccaaaat aaactcatat gagtattcaa ttttaatatt
     1321 agaaatcaat aattcttcca actttaaaat caatttatct atagataaag attcattaaa
     1381 tattttttta atttctaaat ctaatgaagt attaattata gaaaatggaa caactttatc
     1441 aataagttca ctttgctgta tacctataag cgggaaaaaa ccagcaggtt gaaattctat
     1501 aattagtata acatcacatc tattagcaat atttcctact gtttttggtt tagtagttgg
     1561 accaaataat agactatgta accctgtggc atcataaaaa aacaccaaag ttacacttcc
     1621 atgaggaata atcgtgtaat tatcagaaat tattgtttga tttggaaacg atatagtaaa
     1681 attcgatatc caatttctta attgtataca aggcaagaga taaacaaaat tgtcttcaca
     1741 aactataagt gacctatttt tatttataaa atgaatactt tcttttttta acatatatta
     1801 tatcctgtcc tatcgtgaat atttattaaa gtaagaagaa tccatattat ctctatatat
     1861 cattaactgc ttttaataat agagatagta tgaatactca ttagatattt aataaacaaa
     1921 taaaattaaa ataatcaata aaaaatatag tacttttatc tgttatttta atattatagc
     1981 acatttatag tatttaactt atatccactt tacatatgtc taatttatat aattaaaaat
     2041 gcttatctca aatacaataa ttagtcctta tacaattaaa ccatctgaaa ctgtaatagt
     2101 acgactacta cttttggctg cttctggtga gtgagttacc ataattatag tttgtccatt
     2161 gtctctgttt attttattca gtaaatctat tatttctatc cctgttttac tatctaaatt
     2221 tccagtaggc tcatcagcaa atagaatctc tggttttcca attaaagctc gagctatagc
     2281 aacacgttgt tgctgaccac cagataactc tcgtggtgta tgtttacgtc tatcagttaa
     2341 accaactata tctaagattt catctaattg atttttataa tcttttaaat ttttaccatc
     2401 taataacaaa ggaagcatta tattttcctc cacatttaaa tttggtatta agttataaaa
     2461 ttgaaatacg aatcctatat ttctacgacg tattatgctc attttctcat cattaaaatg
     2521 tgatatatca gctccattca tatacacttt tccgctcgtt ggagtatcta aaccacctaa
     2581 aatatataaa agagtacttt taccagaacc agattgaccc atgatagaaa caaattcacc
     2641 tttcattact tgtaaagata cctcttttaa cactcgagtt gttgtatttc caattttata
     2701 ttctttgata atattttttg cttctacagc taaactatta tgtgtaattt tcatattaac
     2761 ctccataatt attcaaactt aatttcttct atcagtttca ttttcttccc ttttataata
     2821 ggcactatcg aacctattaa agttactata attcccaata acccaacaat aataaatgtt
     2881 ttaaaatcta aatctggttt cattgaaatt tttggtcctg caactataaa gatagtttga
     2941 atctcaagta tagatatcac aataccaata acagctccaa gtaaacctga agtaacccct
     3001 tcaatcaatg ttacttttat attttgttta ttacttagtc ctatagattt atacatagct
     3061 atagaacgtc ttttttgaat ataattaatc agtagattat taataatacc tactgtagca
     3121 agaagaaaaa tgaaataggt catcttattc ataggagaca aaaaactact tatagtattt
     3181 aaagaatcat tattaaattc ttcaactgta cgactccaat tatatgtatc tccaaataaa
     3241 tatcttatct gaatcataac agcatctgga ttgactgctg tatatactaa aaaaccataa
     3301 ttagttttat caaaatcatt aactgcataa tgtgatggta taacagcttc tacatcattt
     3361 gcacgggatt taaaactacc tactatttta taagaaaaat catacttatc attagataga
     3421 gttattgtat ctcctataga taagcctact ttttttagag tatcttcatt caaaataaca
     3481 gaacgtttat cattaaaagc ttctattact tgttttttta tctcaaaatt tgtataattg
     3541 attccaaaca tagagttata tagttttata ttatctgtac cctctagtct tgataatgtt
     3601 acaccattac cagaaatctc attattcata acataaagag gtaatatttt tttaattcct
     3661 tccatatgtc taacatcttc aataaactct tcattcatat ttccatctgc aaaaccttgt
     3721 aattctgcgt cacgaaacac atctgttata taagttttga caaagttacc aacaacactt
     3781 attgcaataa ctgcagaaat acttataaat agtaaagtga tattctgtat tatatttttg
     3841 ttatttttca tatttctagc agctaattta ccctcattcc ctaaaatatt tttataaaca
     3901 aactcaaaaa ctatagacat gatatcagta ataagaggaa tcaacacaat agtagctaca
     3961 attaatccta gtaatgaaaa tccaccagct agatacaatg tattttcagg ggatattctt
     4021 ggaagtaaaa tagataaaat aaacattata cttcctataa aaagtataga acgatttgaa
     4081 acattctttt cttctactgt tcctagtaca atatttttta tcggcaaatg gctagctttt
     4141 ttaacaggta tatatgcact aaatgaagaa acgattattg ctactataac agatataatc
     4201 actccaatgg gtgatattac tactggaatt gatattcctt gttcgagaga atttcctaac
     4261 ccatgaagca ttaaatttaa aacaactaca ccaataggaa tagcaattaa tcctcctata
     4321 cttccgtata atatactttc aagcatcaat atacgagtta cagttttttc attggcacca
     4381 atacttctaa atgttccaat aaaaggaaga cgttctaatg taataacctt ataactgctg
     4441 tatattataa atatactaag ggttaatgca aaaaaactta ttaaaaagaa aggcattgtc
     4501 ttttgtctag catctgaaat tatcttagtt tcatttatag tatttgatac catatatttt
     4561 tcagtagaaa gttcttcgga taatttattt actagatttt ctgtaatttc ttcttctgac
     4621 tctataagaa ctcttgtata tccactgcct ttatttataa tttttgatag agtctctttg
     4681 ggtagcagtg catttacacc tcttgtgttc ctcaaaaata ctgtatcata agatgcaata
     4741 tcactaactt gaaaagtata agactttcca taaatctgca aagtaataga atcacctttt
     4801 tttattttat attttgatgt aaagcgattt ggaagaataa ttttatcacc agaaaaatca
     4861 gtaatactat cgccattttc taatcgtggc ttgttgattt tatttagttg acttaagtct
     4921 gctgatataa gactaaattc ttcatagtat ccccctttat tataaatagc agagctttcc
     4981 aatacaccaa cctttgattt tatagtattt aaatcaggta tgtcttctaa attggttaat
     5041 atatctggat ttttactttg gacagatata gtcgctgtac catacatacc ttttgccatt
     5101 tttctttgtg cactttcata agataatcca attgagaatg aaacaaaaag taaaactgtt
     5161 gataaaacta tggacaataa cataactgct gttctaattt ttcgttcttt aatatttgtg
     5221 aaaatatact ttaatataat tttcaaactt acaccttctt cccttatcta agatatttca
     5281 atctaatatt tttatacttt aatctaaatt aaatctctaa tagactcctc catactttta
     5341 tcattctatt tataatattt ttaatcaaat caccccttaa ataaaaaaga ttgataaact
     5401 actatataaa tataataatc tatcaacctt aatatatttt ttatttaacc ttaattaacc
     5461 ttaattaaat aggaaaggtg atataaaatg agctacctaa tccaggtata ctttcaacag
     5521 atatattccc tccatgaact tcaataattt gcttcgttat tgctagtcct aaaccagttc
     5581 cttctatttt ttgttttgta ttgcttcctc tataataacg attaaacaaa tttgatgctt
     5641 cttctcttga catccctttt ccattatcag atataatgat ttcaattctt tcatttaatg
     5701 ttacatgtac acaaacttta gtatttttat ctccatatgt aaaagcattg acaataagat
     5761 tactaaaagc tcgttttaat agtgtacaat caaaatcaaa gttaatatta tctatattac
     5821 tcttaaattc aactatattt gattcatatt ctggataatt taaaatatca atggataatt
     5881 ctttcaagaa tcttactaaa tcataattat ttttatttaa agggattatt ccatttttta
     5941 attgataggt taattttaaa tcgtttataa gtgtatttgt atattctatg ttctttaaca
     6001 tgattgaagc atatttatat actttttcag gtgaataatt agtactacta tctgtaagaa
     6061 gctctgcata cccttttatt ggtgaaagtg gagtttttaa atcatgagtt atatttgaaa
     6121 tccactcttc tctcataata tcagttttat tttgtatctc atcactttta tgaatttctt
     6181 catttaaaat atttaaacta ttatatatat cagcaaaaac acctttaata ttaactggaa
     6241 gatagcttcg ttttgaaata tcactaacag cagatgtaat atttgatatg cactttgtta
     6301 cccagtaacc ataagcaatt ccagaaacca aaattattaa aaatataatc cctatgattg
     6361 aaaatacaat tgacttacca cctataaatt tacctccatc aaaaaacata ttcactttag
     6421 atatatttaa tggaaaatat ataatataaa tatagtcatt tttattattt ttaattgtat
     6481 taaagtatac agttccatta cttgaattat tttggttagc taatgctaat aattcagaat
     6541 tagaataata atctttatgc tcttttggtt ctaaaaaccc ataaactcta tttccactag
     6601 aatctaaaat ttgcaaccac aattgatttt ctcgaagttt ttctaatcca gactgtttta
     6661 cttgtggctt attatctata aatacgattt gttcattaaa gttttctgta aattttttcg
     6721 gccagtcact acgaataaat tccccatttg gtttctgaat agtaataagt gataagaaaa
     6781 tacctattac tgtcaggatt aagcaaataa aaatcatgaa aaaaataatg tatgaatgaa
     6841 atactgtttt ataactaggt ttatgcattt aatcaactct tttcttaaac ttttatttta
     6901 gtttgattgt tgcttatata aaatagacaa taattattga tatgtaaaaa tttatttaaa
     6961 aatacaacta tctacataaa ttatatttat ataattacat gttatcaagc tgtctgttgt
     7021 atttaataaa tcattttgat aacatataaa agtaagataa atttttccat taaatacctt
     7081 ataattatac acaaaatctc tatatacgtt aaacaatata gagaaaattt tcatagaacc
     7141 atctttatag gggatacttt ataaaagaat aaaaaaatta taatatataa aaaacttcat
     7201 aggagtgtaa caacatcagt aaagatatga aaacactata taaaaattca aaaatattaa
     7261 gaataatgaa atacttgaaa ttgcttatat caagaaattt tatataatat aaactggtca
     7321 aatatgttat aatattaaat tgtgacaagt tcatggaggt gtttatatga aaaaacaaaa
     7381 gtcgccaatt ttacttgaaa tgcttggact attttttggt tgtatatcaa tgagtattgg
     7441 tataaatatg tttttaaaac cacatacaat tgctcctgga ggacttagtg gtctatcatt
     7501 agtactaaac aaggttacag gacttccagt atcagctatc atgttaatta taggtgtccc
     7561 attagtaata cttgctttta gaattatggg tacaaaaaat tcattaaaaa ctttatttgg
     7621 aacagttgta ttttcagcta tagttcaatt aacagaccca ttatcaaaac taagatttac
     7681 taatgactta ttattgtctt ctattgcagg aggaatccta gttggtatag gtttaggaat
     7741 aatgtttaaa tcagatgctt ctactggagg aacagactta attgcactca tacttagtaa
     7801 gaaattccca ggaataaaag ctacaaaatt catgtcatgc ctagatggaa tggttgttat
     7861 atcttctggt atagtaaatc gtagtttaga aactgctctt tactctggta tagcactttg
     7921 tgtattaata aaaatagcag atatgataat ggaaggattt gaccattcaa aagcattttt
     7981 tattatctct gatgagcctg aaagtttaag acaagctata actgaagaac ttgatagagg
     8041 tcttactatt cttgatggta aaggtggata cactagagaa aataaagaag ttctattagt
     8101 cgtagtttct aaaaaacaag aactttatct aaagagatta gttaaaagaa ctgaccctac
     8161 tgcatttgtt attgttacag atgtacatga ggttcttgga gaaggattta aaaatttaga
     8221 gaactaataa aaattaatat tttaagtata tatactattt taattaaaat acgatttatt
     8281 caaaaagagt gtctcataaa ctttttagtt catgagacac tcttttttgt ataaaatcaa
     8341 atgcattttc agtattttaa caataaaaac aggattatct atattttgag gcaaccactt
     8401 tattgaaatt ttatttagaa aacaatcatt actaaataac cagcatagat attaaatgat
     8461 tatatctatt gttttttcaa ttcatattga taacaaggaa cattttttac agtatttcca
     8521 gtatgaattt ttttgatata ctttcctgta catttaaatc ctaataaatc taatgatttt
     8581 gtagaatttt ggttttcttc ataggttatt gcaattattt tatttaagtt tagttcccta
     8641 aatccatact gtattactct ttttgttatc tcagtagcaa aatcatgtcc ccaatagctt
     8701 ggatttatac atatacctat ttctgcttct aatgctttag gatttatatt attaaacccc
     8761 atattcccaa taactttatt gtctctttta tatactatag caaagtggcc aattttccct
     8821 tttttatatg gatttagaaa aaatgatttt ataaactttc ttgtatcttc taaagattta
     8881 tgctcactaa atggtaaatg ttttactact attttatttt tataacagtc aaaaaaatct
     8941 tcaacatcat ctaatgatgc aggtcttagt atacaacttc tagtttctaa ggttttcatt
     9001 tgaggatatt cagaatcaga catttctaat ccccctttat acattaatct aaatatactg
     9061 ttcatattta aaatacgata ttaattatat aaatgttctc aaattaacct tatttttatt
     9121 tttaataaaa actaattact atatgaatct ttcaaattta cgaatttatc tctattttta
     9181 tattaaatta cttgtgaaat atgtattcta aattatatat tattagatat catgcttaaa
     9241 aattctttgg ttgctaagga taaagatata tcttttaaat ggcaataacc tatatttctc
     9301 ttaggtattg gctcttttat atctaattta aacaatttct cattttccaa ataatctatg
     9361 ctaaattctt ctataacaca agacactcca agatttatat acgcaaattc taaaagtaat
     9421 tcatgagaac ccaattctat atctgggttt agttttatac cttttgataa aaaatattca
     9481 tttacataaa gtcttgaatt tgctttattt tctagcatta ttaaaggcaa tgtgtttaat
     9541 tcttccaatg atattttcct acctttgtat tctatgtaat catttgcaca tacaaatata
     9601 tcatgtattt caatacactc aacaatattt agtgtatcat cttctattgg catgtttata
     9661 attgctatat ctatattccc atctttgagt aaagatataa gttctctact cgttctattt
     9721 attgtagata catgtatatg tggatacttc ttatgaaaaa tttctaaata ttttaacaag
     9781 aaaaaacgtg cagctgtatc tcctactcct attttcagag aaccgtattc taatttttta
     9841 aatttattta ttctatcttc tcctgatgat attaacttta atgcagtagt gacatgttca
     9901 cttagaattt tcccctctgg agttaatttt actcctttat tgtttctata aaataataaa
     9961 gtatctaatt gttcttctaa ttgctttatt gactggctta cagctggctg tgacataaac
    10021 aattccttag cagcaagaga aaaactctta ttatttacaa ctgcattaaa aaccttataa
    10081 agttcaagtt ttatattcat atataattac tccttatgtc aaatattagt attatttatt
    10141 ttacttataa ttatatttga attataatat acatggtact aatatgacaa tattaattct
    10201 ttaggaggat tttagataat ggatagatta gttggaactg tttcaagagg ggttagagca
    10261 cctattataa gacaaggaga tgacttagtt aaaatagttg ttgattctgt tttaaatgcg
    10321 tcaaaaagtg aaaactttga agttagggac aaagatgtaa ttgccgttac agaagctgtt
    10381 gtagctagag cacagggaaa ttatgctcac gttgacaata ttgcaaaaga tgtaaaagat
    10441 aaatttggag atgatactgt tggagttata ttcccaattt taagtagaaa tcgttttgct
    10501 atatgtttaa aaggaattgc aaaaggatgt agaaaagtcg tattaatgtt aagctaccct
    10561 tctgatgaag ttggaaatca cttaataagt attgatgaac ttgatgataa gggaataaat
    10621 ccttggtcag atgttttaac agaagaaaaa tacagagaac tatttggata taacaagcat
    10681 acatttactg gtgtagatta tgttgattat tataagaatt taatcaagga ttgtggtgca
    10741 gaggtagaaa tagtatttgc aaacaatcca aaaactattc ttaactatac aacaagtatt
    10801 ttaacttgtg atatacatac aagacaaaga acaaagagaa ttttaagaca aaatggagct
    10861 aaaaaagtat attcacttga tgatataatg acagcaagtg tagatggtag tggttacaat
    10921 gaccagtatg gacttttagg ttcaaacaaa gctacagaag aaacagtaaa attattccct
    10981 ataaattgtg atgaagttgt aaacaaaatc caaggtaata taaaagaaat aacagggaaa
    11041 gatgtagaag taatggttta tggagatgga gcttttaaag accctgtagg taaaatatgg
    11101 gaattagctg acccagtagt atctcctgct tatacaaaag ggctagaggg aactccaaac
    11161 gaagtaaaat taaaatactt agcagataat gattttgctg acttatctgg tgatgagtta
    11221 aaagaagcaa tttcaaaata catagttgaa aaagataata aatctgatga tttaacagga
    11281 aacatggttt ctcaaggtac tactccaaga agattaacag accttatagg ttctttagca
    11341 gatttaacat ctggaagtgg agataagggt actcctatta tttacataca aggatatttc
    11401 gataactata caaaatagta tgttttaata agatttaatt tttttggatt atatatatta
    11461 acatgaaaat ttattaaaac ctcttaatag atatgacttt tcaagagtta tatctattaa
    11521 gaggtttatt taatgttcac agataagata tatttaaata atttaaacaa gattgtcaga
    11581 aagttatata atcttaaaaa tttatataac tctaattctt atttatttct gaaataatat
    11641 cactcttcaa aatttgtctt actggaataa ttacagcaac tatactaata gtgatattta
    11701 aaataatcat acctatgtat agaggtgtat ttattttaag aattggattt atatataaat
    11761 atacacgttt taccataaaa taaatcataa attgtccaat aacacttcca attaccatta
    11821 taatacttgc atagaatcca tataaaaaac cttctcttat catcataagt aaaaacttat
    11881 tatccgtaat acccatagct cttaaaattc caaactcatg tcttcttgaa aaaattaagt
    11941 agcttactgt attgattata tggaacaaac ttattgcaag aagtacaaat acaataccat
    12001 atataaataa gagtttttga tttaagtagg tattattttt ttcaatagca atggtataat
    12061 ctgttacgat acatcttttt acatcactta caacactttt tatgttctca gacaattcct
    12121 cactgttact atcttctgtc ttttttatac ttactgaatt ataattttcg atatcaaagt
    12181 tatttttcat ttgcccattc gtcataacta tatccatacc ataatcacca atgaaatata
    12241 tgttatttgc catcacacgt tttacagtag cagcaactac aaattctttt gttgtataat
    12301 cattcttatt ttcttcttta aatttaacaa tttcatctgt tggtttcatt gtctttggca
    12361 ctttaacttt tattgtatct cctggattta tatcaactgc atcatacatc ccagttccat
    12421 ccataggtag tctaactata accttattat ttttaaccat atcatgggta ttaatctccc
    12481 catctataat ataagaattt aatccttcta acataccatt gttataacca taaatatttg
    12541 tttttaaaag atagccacct tcattttgct ttgtacaaat accatcaaaa aaagtcttta
    12601 tcctgtaatc tttttcaaga ggtttaaaga aatttttcca aaatagttgt tcttctttaa
    12661 tttgcatagc tccaaataaa tatcttactg gatagacttt tgatattccc tcaacattgt
    12721 ttaatcttga aattttatct tttggtatac ctacatcaaa atctgttgtt tgcatagtta
    12781 tttggtagtc agaatttaaa ccatcatcag ctttcattgt aagttcatta ttatttttag
    12841 taagctgtat agagtaacta gaacataaaa atataattcc accaagtgat aatgaaaaca
    12901 aaattccaat aaacattccc tttttttgag tcatgtattt atgagataat acttttgtca
    12961 tgttagagtg ttttacactt tctaatattc tctttttctt attagtaatc ttaaagtttt
    13021 tcatagattc tatactagag tatttattca actgatgtac aacacgtaca gcaattaaaa
    13081 ttaacaacaa taagataaat aaaaagccta aataaataga tttttcactt ataaaaaatg
    13141 ctcctggaac aatatcacta ctcaaaaata tatgtggaaa ttgttcatat attgtagacg
    13201 ccacttcaat acctaagaag caacctatag gaaatccaac aataaaaagt aaaaataact
    13261 ctaaaaatag tagaaataaa agttgtttat tattagcgcc taatacttct aaaaccccat
    13321 attgtgatat tctctgaagt attgaaacat ggaaaatact atatactgct gctgcaccaa
    13381 acactccaac aagtaatata attgcatttg tatttagttc taaatttttt aagaactcaa
    13441 atatattata tttttctata ttttttggtg gttttactcc aaattcaact gaaagaggcc
    13501 agtttatata agttttttct tttgatatgt tgaatttttt cataaattta taattttgct
    13561 ttttaatatt tttattttca tcatatttta cataaactat ataagaatcc ttttctttag
    13621 gctgaaaata atcgtttaca aatccctcca gagcagtttc tgggtttgaa agacttgata
    13681 aaataccaca taacttatag attttaccat ctaaattgat ttctgttcca atttctttgt
    13741 ttacatttaa attgtttaga gaatatgtgt caagtactat ctctccatct ttactaggaa
    13801 gagttccttt taataatttt ctacctgtca tgtctaaata tgaagaatca gcatttaaaa
    13861 attctataat aaaaggttca tctatctgat ttttaggttt tgtaacgcca agtctatcta
    13921 cagtgtattc ctttgatttt ttattttttt taattttatt aagttgttcc ttatctactt
    13981 tatagaaata atgataatct ccactatctt cccttatctt ttcaattcta cttttgtctg
    14041 cactatgaac taaagaacca attcccgtca ataatgctac tgatagtact atacttaaaa
    14101 ctatagatat agattgtttt ttataatgct ttatatattg aattgaaaat tttactagag
    14161 acatcatagt acttcaccac cagtattcac cacaactcca tcttcaatat ggataattga
    14221 gtcgcatact tgtgctatat tttcattgtg tgttaccata agtatcgttt gatggaattt
    14281 tgcactactt tcttttaata aacataccac ttccattgta gttttactat cgagatttcc
    14341 tgttggttca tctgctaata taagcgcagg tttatttgcc aaagctctag caattgctac
    14401 cctctgttgt tgaccaccag atagttcgct tggaaatttt aaacgcttat cccaaattcc
    14461 caatgtcttc atcagcattt caatatattc atggtctata tggtttcctt tatctaatgt
    14521 aacaggaaga gctatattat cataagtgtt taatacgggc attaaactgt agttttgaaa
    14581 tataaatcct atatttcttc ttctaaacac tgtcaaatct ttttttccta ttttagatat
    14641 gttctttcct ttaattatta catcacctga ggttggatta tctaatccac ctataatatt
    14701 taacaatgtg ctttttccag aaccagaagt tccaactata gcagtaaatg tatctcgttc
    14761 aatttctata cttactccat ttaaggcttt tactttagtc tcaccttttc catagtgttt
    14821 tactaaatta ttcgtcacta atatactcat ttaatttctc ctttcaatac agatatatgt
    14881 tctataaaat attgtaagtg acgataatta cactcatcct acaaaattag tttaaactct
    14941 tacaattctg taagaagata ctaaaacaac atccacttcc tagtttagat tctactataa
    15001 tgtttccatt ttctttttct aaaattaatc ttgtaagata tagaccaatc cctgtgccat
    15061 tttggttttc aacattttta cttctgtaaa atcttttaaa aatatcattt agttctctac
    15121 tatcaatacc tattccctca tctttaatag taatcttcgt atatagttcc atttttatta
    15181 cagaaatagt aatcgtgcta tctgatggtg agtattttat tgcattttct aaaacattta
    15241 caattgcttc tatggtccac tttttattat gaactaattt tatgtctgaa aatggttcga
    15301 gatttatcct tatattttta gcttctgctt tattaaagat agtactgata ctttgaataa
    15361 gagtatcttt aattagtagt tcttctactt caaattctat aactccatcc tctaatttag
    15421 tcattttaaa taaggattgt aacagccagt caatcttaaa agtttcattt ttcatctttt
    15481 gtaaaaactc cttcttctgt aatgtggaaa tatttatatt ctccagaagt tcacaataaa
    15541 tggttatatt tgaaagtgga gtttttaatt gatgtgacat atttgatatt aaacctttaa
    15601 tcgcttcttt ttctgacttt gactgttcta cctctatttc tatcatatcc ttaatttttt
    15661 ttacttggtg agaaattttt gacgctcttg tatcctttgt atctggataa gtaacttctt
    15721 taccttccaa tatttgattt aacatcaaat ttaattcatc atatcttttt cgatttattt
    15781 tagtagatac tataaatata acaactgtac atattataaa ggctaatata aacatattat
    15841 tctacctcat tccataaata acccattcca taagctgttt ttatatattt tggattagat
    15901 gggtcatcct ctacttttac tctaagtctt cttatattta cagatacaat attatcatca
    15961 acataatttt cttcgctgtc ccaaatatgg tgtaaaattt gttcctttaa taatatttga
    16021 cctttatttt caattagata acttagaagc ttatattcta cagagctaag ttccaaacat
    16081 tctttatttt tatatacttt tagtaacttt tggtccaatt ttattccatt ggaatatatt
    16141 actttattat tcgatttttt tcttattgct actttgactc ttgcctttaa tacagataaa
    16201 gaaaatggtt ttgtaatgta atcatcaagc cctatttcta atccttttac ttcatcaatt
    16261 tctgagtctc ttgcagtaag cattataatt ggtatatctg acttttccct tattatttga
    16321 catacatcaa atccatctcc atctggaaga ttgcagtcta agattataaa atctatattt
    16381 ttactattaa atatatttaa tccttcttgt tttgtataag ccttaaatac attaaatcca
    16441 tcattctcaa atgcataaaa aagaccttca tttaagttta tatcatcttc aataattaat
    16501 aagttcaatt ttactctcct ttagattttt tagataaata attattttct tgcattatca
    16561 tactaaaaat attttaattt tatttatttc actaaaaaga ttgtatttaa atttgttttc
    16621 aaaataaaaa cattataatc aagcatatag ttatatattt cccaatttaa ttacgcttaa
    16681 attgaaaata taactttatt cttatataat taaattatac aacaataaag ttatattttt
    16741 taaatatatt tttactaggg ggaatagaat tgaaaagggt tgatgagaga aatactatgt
    16801 ttgcaagagc aaattacaaa aaagatagta ctgcttataa tgactattat aagaaaaatc
    16861 ctgacaaaaa agaaattgat gatagtataa gaaatagacc aaatttatgt agtgaaggaa
    16921 caatgactta caatgagtta aactctccaa tggcaagtag tgcattcgat tttttatctg
    16981 atatcaaatc tttatgcgaa ggaaaagttt ctgatactaa agtagatgta gatgcaaagt
    17041 caatgacaaa aaaaattaag ggtcttgcaa agcagtatgg agcttctgta gttggtataa
    17101 ctaaattaaa agactatcat atttatacaa ataggggtag acatgaagaa aattacggtg
    17161 aagaaattaa tctaactcat aagtatgcaa ttgtatttgg atgtgagatg gataaagaaa
    17221 tgataaatag agctccaatg atatgtgaag ttatagaaac atcaaagtgc tatgtggatg
    17281 catctatcgt cggcatgatt ttaagttact atataagaaa tttaggatat gatgctagaa
    17341 atcatatgga tgctaattat ttagtaatgc ctgtttttat agctcgtgat gctggtttag
    17401 gagatatagg aagaaatgct atacttacaa ataaagatta tggctctaga cttagattag
    17461 gtgttgttac tacagatatt cctcttttag aagatgaata tgttgatttt ggactagaag
    17521 atttttgtaa ggtctgtaaa aaatgctctt ttaactgccc ttctcattcg ctttctaatg
    17581 atattaaaat tggtgatgat ggaaaatata attgggtaat tgaacatgag acatgctata
    17641 ttaaatggag atatttaggt acagattgtg gtatgtgtat atctgcttgc ccatttagcc
    17701 aaaatctcga aactataaag aatactactt catttaaggg taataatgag cttattcaaa
    17761 aagctttaga tgagtatact tcaaaatttg gtaaaagaat atttatacct ggaaatccat
    17821 cttggttgaa ataattttat ttaataaaga atctgtacta gaatttatca taggacaggt
    17881 tctttattaa atatataaat ttttaaatac tttttctcgt ttataacaat tcagcaatgt
    17941 ttttagtcta taattgacat tataaaatat taaactaaaa ggagtaactg tatggaaaaa
    18001 atattagtag tagaagatga ttctatatta aataaaactc tatcatataa cctaattgaa
    18061 gatggatata taattacttc aaaatttaca gctaaatctg cactaaagtc tatttttgaa
    18121 tgtgaatttg atttaataat tttggatatt aatttaccag ataaaagtgg gtttgaatta
    18181 tgtaatgaaa taaaggaaaa ttacaatata ccaataattt ttttaactgc taatgatatg
    18241 gaatgtgata tgattaaagg ctatgaactt ggagctttgg attatataac aaaaccattt
    18301 aatatcaata tatttaaaca aaaaattaaa gcttttttaa atcatcttac aataaaaaca
    18361 aaacaagatt actataggga tggatatctt gaaattaact tttctgagtt atctgccaat
    18421 ataaatggaa atcaaattat atttacacca ttagaatatc gtactttaaa actgcttact
    18481 gaaaatccta aaagtatttt aacaaggaaa gtacttttag aaaaactctg ggatatagat
    18541 gctaactttg tagatgaaca tactttaaca tctgttataa gtaggattag aagcaaaatt
    18601 gaaaaagaca atcttaagta tatcaaaacg gtatatggta tgggatatat gtggttaggt
    18661 gaaagaaatg aattttaaaa aattttcttt tagttctatt tatttaataa ttgctgttgg
    18721 tataattttt acagctattt tatttagtat ctcaatttat ctaataactg atgatttaaa
    18781 actaattgct ctattattaa tatatacaat agtactttgt ggatttatag cattaataat
    18841 atttttactt cataaaaaaa tagttatatt ttgttctaga atatgtcaca ctttagatag
    18901 tatgatgaat agtgaagata atattgaaat tgaattagaa gaagaaacta ttttatcacg
    18961 tatcaatcac agattaatta gaatgtatga agttattcaa gaaaatcgca atagtattgc
    19021 tatagagaaa gctgatttac aagaactagt ttctgatata tctcatcaaa ttaaaactcc
    19081 tattgcaaat ttaaaaatga ttaatactac tctactaaat caaacattta aaacagaaat
    19141 tcaaaaggat tttttaatgg atatggaaaa ccaattagat aaattagatt ttttaatgca
    19201 atctatggta aaaacctcca gactagagac aggtataatc acactatcta aaaagaaaaa
    19261 ttcaatttat gaaacaattg ctacagcact tagtggaatt ttgtttgatg ctgaaaaaaa
    19321 gaatattgaa gttacagttg attgtgaccc aaatttatat ataattcatg acaagaaatg
    19381 gacttctgaa gctattttta atatattaga taatgctgta aaatacacaa gttctaatgg
    19441 taagatacgt gtagctacag aatgttgggt tatgtataca aaaatagata ttattgataa
    19501 tggaaaagga atatcagaaa gtcgccaagc agaaatcttt aaaagatttt atcgtgaaga
    19561 agatgtccat gacatagaag gtattggaat aggtctctat ctttcaagaa aaattatatc
    19621 tcttcaaggt ggctatatta aagttacatc agatattgga aaagggtcga ctttttctat
    19681 tttcttacct aataaataaa tttttaaata tttcatttgt aatgattctg caacattttg
    19741 gttttatact gtatttacaa gatgttaagg aggtattatt atgagtattt tacaagtaat
    19801 taatttaaag aaatattatg gacaaagttc aaacattaca aaagctcttg atggtgtaac
    19861 tctatctatt gaaaaaggcg aatttatttc tattgtaggt acatctggta gtggcaaatc
    19921 cacattatta aatatgatgg gaggattaga taatcctaca tctggaaagg ttattataaa
    19981 gggtaaggaa ttatctacta tgtctgatga acagcttaca atctttagac gcagaaatat
    20041 aggttttata tttcaaaatt ataatcttgt tcctttactt aatgtatatg aaaatattat
    20101 cttaccaata ggattggatg gagagaccat agataaggat tttataaatg aaattataaa
    20161 tatgctttct cttgaagaaa aattatatga tataccaagt aatctttctg gtggtcaaca
    20221 gcaacgtgtt gctatagcta gagcacttgt aacaaaacca gctattatac tagctgatga
    20281 accaacaggt aaccttgata gtagaactag ttcagatgta ctgagtttac ttaaagtgac
    20341 aagtaaaaaa ttcaaccaaa caattgtaat gattacacac aataatgaaa ttgcacaact
    20401 aactgataga attattcgta ttgaagatgg aaaaattgtt aactaggagg tgtagtatat
    20461 gtttagaaat aataatcgca catttataag aaaaatagct ttaaatgatt taaaaattaa
    20521 taaacttaaa acatatcttt caggtatcat tataatgatt tctacatgtt tattattaac
    20581 agtgacctta gtttcatata atgcctctgt agatttagtt aatgcatctc catatcatgc
    20641 tatatataaa tctgtagatg aaaaagcaaa aaatatacta tataaagata aaaactttag
    20701 tggcgtagga acttacaaat taattggaag taataaaaag acagattgta ttatgtcgat
    20761 tgtttatgca gatgatacag caattaaact tatgaatttt caaccattga aaggcaaact
    20821 acctacaaat aaaaatgaaa ttgctatttc agaaaaatat ttacaggaat ttggtttaga
    20881 taaagatatt ggagatagca ttaaattaaa ttattatgat gatattacga ataaagaagt
    20941 tcaatgtgat tttattattg ttggatttct agaaaattat tatcaagata atgccaagca
    21001 atactataca gtcgtttcaa atgattattt taagaatata gcttctacac ctttacaaaa
    21061 taaaagtaat acttttgacg agtcaagacc tgatactgta gatgtattag ttaaactatc
    21121 agaagaaaat acagagaaag attcagcttc aattaagaca cagttaaata aaattggttt
    21181 atctttaggt attaagaaat ataatatata cttaaatgat aactatattg aatcaaattt
    21241 aattgatggt gagcagatta ttacagtttt agtagttgga atagttgttc ttttttctag
    21301 cgtttttgtt atatatagta ttttttatat ttcagttgtt aattcagttc agatgtatgc
    21361 taagttgaaa tcgttaggta tgacttcttt tcagttaaaa aaaattattt ctctacaagg
    21421 aaatattttg tcaattattt ttattccatt aggggttata gcttcttgta taattgctta
    21481 cataattcaa ccattagcat ggcaaatgat agctgattta tttataattt taattttatc
    21541 actggtaatg tttttaactg ttagaatttc tttatttaaa ccagctagaa ttatttctaa
    21601 aatatctgct attgaagcca tgcaatatac agaaactaaa tttagaaaaa aagcaaaaaa
    21661 ttttagttat ataaatataa aaaatctagc attaaagaat atcgagtcaa atagaaagaa
    21721 aaatttgatt gcattaatat cgctaagcat tagtggagtt ttatttattt caatcgctaa
    21781 tcttgctaat tcaatagatt tcaaaaaaca actatctcaa caatttgtct ataacgaaga
    21841 ttatataatt agcattaaga gtgataactt atatgagcat attacagaaa tccaacaaaa
    21901 taatccacta acagattctc ttaagaatga aattaaatct attcatggag taaaaaaatt
    21961 aattgaatcc aaatcaatta aaagtaatat tatagaacct tacatcaaag atagagacga
    22021 aaaacaattt attactctaa ttaaaggtat tacgcctgaa ctttcaaaat cacttgaaga
    22081 atatattgtc agtggaaaaa tagactatac acatttagat tcagatagtt taattataaa
    22141 taaatataga acaaaatatt atggattaaa tttaaaagtt ggagatacag ttacatttaa
    22201 tatctttagt ggaaatgata ttattaaaaa gaaaatgaaa attataggaa tcattgacaa
    22261 ttccaataca ggaggtatgt tttttgcttc agacaaaact ttagaatctc taacaccata
    22321 taatacaaat ttagattttt ctattttaat agataaaaag cattctaaag aagttgaaga
    22381 aaagttgtta agtataattc agaaaaattc aaatttaatg ctttattcat atgaagatga
    22441 ttatagaatg attactagag catttcaata tataattata gcttcataca tttttgttgg
    22501 tttaatttct tgctttggta tactaaatat gaccaatacc ctaataaaca gcgttttaat
    22561 tagaaaaaaa gagtttgcat tacttcaagc cgttggaatg acaagaaagc aactgcgaaa
    22621 tatgctttat agagaaggtc taaacataag tatcaaagct atatgtactt caagtatact
    22681 aggttatttt ggttcaaatc tactttgcac ttttataaaa gatgtaatta gattagactt
    22741 tattaatttt aaattttcta tatttactat tttaattttt tcattcgtat taattggtat
    22801 acaagtttta gtgactgaat tacttgtaag aaatatagaa aaaaaatcac ttacagaacg
    22861 cttacgttct cagtaagaat gtatataaaa aatagccatt ttttatacaa gttttaacat
    22921 aattttttaa aagctaaaag gatagaaaat tttgacttta cataattttc tatccctata
    22981 cttgttttta ttttatacag atacacttac ttatatctcc gagcaacatc accagagaca
    23041 taaacaagca tattccttat tttctaaata tgtcaattgc atgacttgta acccctacta
    23101 aatcttcacg ttcacaagta gcaaggtgat attttaaata taatgcaaat gcatcatcat
    23161 ccatagagtc tactgcttca cgcatataca aagccaaacc atctgatgcg acataatgca
    23221 gtctagttac attaaatgca gacatcaaat catcaatgtt ttctttacgt acaagctcaa
    23281 acaaatcctt tggttcagac ttagcagcaa atgtctgtgg atcaatcaaa cctttttcaa
    23341 tatattcaga tacattaata tttcctctat ggaacccctc gtcaataaga cagccatcag
    23401 atataacata tgcagcaaaa accacaccac caggttttgt tacacgaatt gcctcatgta
    23461 aagcttgttg tttatcttct ttgttataaa gatgatatag tggtcctaac agtaacgtaa
    23521 tatcatattt attatctggg aaaacagata aatccatagc attaccctga gtaatgctta
    23581 tattttcatt tgattgagta tttttatgga aaacctctat attatgttcg actaattcca
    23641 ctgcgtcaac atcatatccc tgacgtgcca gtgtgtgaga ataacgacca gttgcagcgc
    23701 caatttcaag cacacaatca cctgatttta tgtacttatc aatgtaatgc atagtagtga
    23761 gaaattccac cattccatgt tttaatgcaa gtcgactatc ttcatcataa gtattataaa
    23821 agtcaattaa gtattgtttt gtttccataa tttcacctct tctatattag ttaataaaca
    23881 tcaaatagac aaattccaat ctgatttttc ttgtcttata aaagctgtta atatattttt
    23941 caacattttt tcagcactat atctatctaa tgttaatttt tttgttgctg ttaagacagc
    24001 tatgccatga gtataaagaa ataaatctaa aaatattact tttgcacatt ccaaatctat
    24061 tccaatgctt tccgaaaaaa gtcttgcctt tttttcattg tcaatttctt tataaaaatc
    24121 attcgcctct ttcatcatca aatctaaatc atttataaat aacaacttaa ataaatacgt
    24181 ttcttcttga gcaaattcaa tgtatgaaag cggaagcagt aaataagggc ttacactttt
    24241 agaattacga taattaacaa catactgatt ataatattca tatgcaaaag caagaaattc
    24301 attttttaat tcattcatgt tttcataaca agtaaaaatt ggtcgagtgg aacactgtaa
    24361 tttacttgca atactcctcg catttacagc ttcaaatccc acttctcttg taatatccaa
    24421 gactgtattc aaaatcattt cttttgttat ctttacttta ggtggcatat atgttcacct
    24481 ctccattaaa tttttataaa acacatgtgt tatataacta tagtaacata tcaagaaatt
    24541 atgtcaagaa aaagctttgc atttttttgc aattcctaaa acttataaat aaaacatgag
    24601 ttgcaattaa tctattaata gataacagca actcagattt tcctttaaat actcttcttt
    24661 ttgtctaata tttcatatac acaatcttct attgaattaa tatctttttc ttttcttttt
    24721 ttagaatcaa ttaaaatgaa atacagtata aaacctacag caagtcccca tgttccgcct
    24781 ttagcagcaa ttacacctcc cataagtcca atcaatccct tctcaacacc tgtattacat
    24841 agctccattg atagctttgt acataaatat ccttgaacta aaagtgttac tgacaatgct
    24901 aatggtataa gaggtgtagc aatctgcgca attggataaa aacagataaa aattgctgaa
    24961 acccatacta atgtacccat gccactaaaa acagactgca tggcttcctt tccaaccttg
    25021 tacctttgag caacacttcc tgtaagtgtc gcagctaatg gaccgcacat tggtatatat
    25081 ggagaaaaga ttgccataaa aatatttctt atcccactta taacattaga cctatttgaa
    25141 ttgaaatcta tatattcatc ttttctattt tcactttcag aaagtactaa gttttcacct
    25201 gttataaaat caccaaaggc aattatgtat atcataaaag ccattggtat cccttgtata
    25261 aataacatta cacttggaaa acctattcca aaaggagata ctgtatttat cagattttta
    25321 aaatctacta tgtaaactaa attgtcaaaa ctaaatctag gaatttctat ttctttacta
    25381 aatataccta ctattaaact cactaaaata gctgatacta caccatattc tcctaaatga
    25441 aataaaaatt tattcttaac ttttagagta tcaaatcttt cagaaaacat tacaaaacaa
    25501 gcaaccaaaa ttcctactgt tatagatata ggataagaat caaatctccc tttaggatta
    25561 aactctgata ttatagtcga tataccgaca cccattagta ttccaccttt aatagaattt
    25621 ggtatcttac taactaactt tccagctaca ccagttatac ccattaaaat aaatattatt
    25681 cctaaatcta tttgtaaagc tgttaatgct tgtattcttt caatgcccat gctgaacttt
    25741 aacaaatatg cagttgtaag agttatagaa gctgtaatcc agccaggtac aactgggtct
    25801 cccataaaaa catgcaaaca gtataatatt gagttaatga tagccatact ccatgccact
    25861 tcatagggta atcccaaaat ttgtgtacta aggccaattg tgcctaaact tgtcatactt
    25921 gatattatac cttgaatagc ttctggtttc tcaaatctgt aatgtataaa tggaattctt
    25981 atcttaaaca ttccaaattt tatatgaggt tgttcccttc tagcgagtct ttccataact
    26041 tcactccacc tttcaataac tcttataagt ttacaacttt ataattgttt ataaattatc
    26101 aatgtcatta aaattagtat ataacatgca aattatataa acaataaatt aaatctataa
    26161 aattgataga ttttgtgcat atatagaaag accgcttcat ttttagttac ttaaatcact
    26221 taatagtagt tatttaaatc acttgataaa ggttttttat aaaattcaat agttctattc
    26281 catgattcaa actgtccttt tgaatttcct tctacagttc cacctgaaga aaaaatccct
    26341 ccactcatac tcatacataa agtctccttt agaggcataa tatatggaat tggtaacgga
    26401 tgacccatat tattgtatgt taataattct acgttatatg gataattatt ttcttcaaag
    26461 atttctaatg cctttaaaca agcttcataa ctattccata tgttatcttc ttttccagca
    26521 atcataagta tatctgcttt ggaattttct atttttattc tagcctcttt tctattctct
    26581 gctcttttaa gacttttctt ataagtacta ttaaatccaa atggaatacc ttttgatata
    26641 ttttttttct gttcttcaaa gaatatatca ttgtctactt tgatatatgg aaatggctct
    26701 cctttatatg accatgatga agtatcattt ccactcatca atccattaag agcttggaaa
    26761 caataaatgt gaggttgtga tactacaacc tttttaattt gttcatatct tgaagcaagt
    26821 aaaaaggcca attctccacc ctttgatgtc ccatgtacaa atatttcttt agtactggta
    26881 atttcatttt cactaatcca ctcaaatact ttttcaaaat attctaatgg aacctcttct
    26941 aatttatctg gtaacccttc taaagcaaag tatgcaactg ttagtacatt gaatcctcta
    27001 gatgcaagtg gagctgctaa aaggtctaga gcgtctaaat ttccatcaga accacctagc
    27061 attaaaattg ttttattatt agaattttgt ttataaaata attttcctat aaactcatca
    27121 gatatatatt ttgtggtaat atcttctgat ttaaatattc tctttaattc aacttctttt
    27181 ctttcaatag aagtttcaaa tatcatattc ataattattg gtttatcaat agatatattt
    27241 tctgcaaaat ttttaccttc agttttagat ttttccagcg aataaattaa ccccatacaa
    27301 ttatcagcct tataagtacc ttctataggt tcaactaaat ctaaatctac ttctcctttt
    27361 tcatttgaac aaaacactcc ataagatgaa aattcttctc ccttgcacca tggaaattcc
    27421 attttcacac ttattttaag tttagtattt ggaattaagt ccgaaaccat aatcttaagt
    27481 ctttcatcca caaatgattc tacattgtat acatttactt tcataatcta tttctcctta
    27541 taataattat ttatgtttta tataaaacag ctttatctaa tacaacataa ataaattttt
    27601 aaattaagac accatattga ctatttcatt ttatcaacat attctatttt tgtcaacata
    27661 ttgtcttttt tagcaattta aaattatgta gtctatatga aatccagttg atttttggct
    27721 aattaaagat aggaatgcta ttaggtttag aatatatata ttcaaaataa aaaaccactc
    27781 attttaagaa tggtttttaa tataaactat ttgttattaa taaggatatt tttttattta
    27841 aatttatata atcgaacatc taatatttaa tgtttaataa aattatgcat ttgcaaaagt
    27901 atatatggca ttaactcttg ctacatataa attctcatca ctacatccaa tatatctagc
    27961 atatttttca atcttttctt ttgcattttc aacatcattt tcatcgctct ccattaactc
    28021 atttatataa atatccatta atgacgcttt atattctctt atatcatagc ctaaaaattc
    28081 agtcaatctc tcaatttcct tactaaaatc tccatcttct tttaccaaaa attcgtatac
    28141 atcttcccta tatggattta aagttattat ttgttttact actttgcatt cgtcagcctt
    28201 cccaataaaa ccatctttat aattattaaa taaggcatta gattttttta tagattcttc
    28261 atcaatataa gaatgtaccg cagttatacg attttctata agggcatcta caacagcata
    28321 atgaatttta aaacaatcat tgtataaata attgctcatt ttctttattg tcgattctat
    28381 ttctaagaag ctcgcgtggc catttttttt atttttattt ggtataataa aagttttatt
    28441 caagttatta aaaagtctct taaaatctaa atatttttca caataaacac ttttaaaggt
    28501 attgatatct atagttataa ttccaaaact tactatagtc tcaattcctt tcttaaaaga
    28561 ctcttctata tatttttctc ctaaacttaa acctctatca gatatttgct ttagattttg
    28621 aaaactattt aaacaatctc tctcaaagtt tttagatatt tccaaagcat ataatttaaa
    28681 gattttcctt atcttattat aattattttt tgtatcatca aatctgataa tagctccatt
    28741 tacatcaaaa gtaatcattg agttttaacc tccccattag tttcatcaat aaaataaatt
    28801 taatataaac tttataatca taatattaaa cttatttata ctataattat taatttgatg
    28861 ataaaagcta ttttccttct aaataaaaaa tttgtctaaa gtttctaaaa aatttagaca
    28921 aatcttaggt tattactctt taaattattt ttaaattatt tttaaattat tccaaatacg
    28981 ccaaataata ttggtataaa tatagctggt aacaaattgg ctacttttat attcgttaat
    29041 ttaagtacat ttagaccaag tcctataatt aataaacttc cagctgctgt catttctgtg
    29101 actacaggat cacttaaaaa tccatttaaa aatgatgctc ccacacatat aataccttca
    29161 tacaaaaata cagatattgc agaaaacatt acacctatac ctaaagaagc agtaaatata
    29221 attgaagcta ttccatctat aacagatttt acaaataaag tatcatgact tcctgtaaga
    29281 ccactttcta atgaaccaac tactgccatg gctcctatac agaataataa actagatgat
    29341 ataaaaccct ctgctataga atctttttta tttcctttag aaaatttact ttgtaaaaaa
    29401 gcacccaact tatttaccca tttgtcaata tctattaatt ctcctatcag agcacctaca
    29461 gctacggata ttattgtcac taatgtgttt tttccttcta gtgctcctga tataccaatg
    29521 tacaatacac ataaggcaat tccattcatt atagtatcgc ttactctttg aggtaatcct
    29581 ccttttatta taagacctac tatacatcca cctgcaattg ctagactatt tgctattact
    29641 ccaaacattt tttctcccct cgattagcta attgaaattt atcctattat taaaacctaa
    29701 acatctatac tttgtattgg atattttaaa tattccaaaa attcttcaaa accttttcta
    29761 gatatattta cagttttagt atttatgtta ggatgaaaat ttaagtaatc ttctttaatt
    29821 acatctttat ctatgtaaag ctcaactttt ctttctgtat catttattaa tgaaaaagga
    29881 tttacacttc caggaagaag tttaagtaca ttgtatagtc tttcaggtga agcaaacgat
    29941 aatctagaac ttcctatctt atctttaagt gagtttaaat caacttgttt ttctccttta
    30001 actacaataa gataatactt atttcctttg gcatttctta aaaataaatt tttacattga
    30061 cacccctttg ttattccatc taaaatgact aattgctctg ctgtatatac agcttcatgc
    30121 tctactacat catattcaac atttaattta tccaaaatat catatatttt agtttcattc
    30181 atgctagatt cattttcatc ttgttctttt ttattattta aatcactcat acttttctcc
    30241 ttaaacactt catcataata tcattgtgag gtattattct actatagcta ttgctcttac
    30301 tggagctcca tcagcatcat tatacttaaa tggtgctgct ataaatagaa atttttctgg
    30361 aacattttct aaatttgtaa gattttctat gattattttt cctttttcaa ataaaatatg
    30421 atgcacttca aatactgatg tgtcatatct atccactgat agcatatcta ttcctatgcc
    30481 ttttatatta gtattagcta tatagtttgc agcttctttt gttaatgtag gataacctac
    30541 atagtactgc tttttatccc aaaattttga ccaacctgat ttaaatatta taaaatcact
    30601 attttttatt tcatcttcat atttttttag atattctaat tctatattct catttccctt
    30661 ttcaagttcc aatacataag ctttcccaac aaaattttct atatccaact tatctagggt
    30721 ctcaccttta gtatacatat gtcttggtga atccatatga gttccattgt gagaaaatac
    30781 actaattaat gtttcttgat aaccattttc ttcaataata gctactttct tgatatctgg
    30841 tctattaggc tctgcatata caggcatgtc attatgtgtc acatgagtta aatcaaaaac
    30901 cttcatcttt atataccccc tatgttttta ttttctatta tatacctaaa ttcattttat
    30961 ttctatcaga ttttattcca ttatatacca tactactata tattttttat aaacctcaag
    31021 ggatttaccg aaaaatcttg gaaagaatta tcagtaaata taacatcttc acccaaaaaa
    31081 cgtttatacc ttccttcttc tttctttaaa gtaattctct gtttttgtga atgattatta
    31141 aaaaccatta tcttaagagg aggttttttt ctattagtct gcactctttt taacatgtat
    31201 tttatgtgca tatcagcttc aggaaatgaa tatccacaaa atattaaaag gtcagtatct
    31261 cttaaagttt tttctgtttc attccaaaca ttacttaaaa atatgtttga catattctta
    31321 aaatatgttg gtggcactat aataggttct gttaactcac cacactctaa acattttgtc
    31381 tctgaactat tttcaataag cttcatgaca ccaccttcat gtggtgtaag agttacacta
    31441 ttacaaatgg ggcaataaag ccaattcaat gacccatgta tcttatataa tttgattaat
    31501 ggttcttttg gttttctcca atttaactcc tctttaaaat ttgtaaactc cacaccataa
    31561 tcaagcataa ttggcaaatc attcttatct cttaatccag taactgtatt gtctatatgt
    31621 atatcataat ttgcactaat aaaagtagta tcaagaagca aatcattttc caaaagattt
    31681 tcaactaaaa gtttatgata tttattatct gttctaggtg tattatgaat tgcttcagcc
    31741 attagcagta ttaaatattg tcttaaaaaa cgtatactat cacttctgtt atttagattt
    31801 tctagtccaa aatttctaaa tgcttctttt ctttgttctg ctaagtctaa aagacctaaa
    31861 acttcttcaa aggtaggaaa atttgcatta tctatattat cttttactat atcaatattg
    31921 aacatctgtt taaagaaaat atacaattct ctattcattt tggtattata atttgtggga
    31981 tttagttttt taaaatattc gctaaaaagt tcattttgaa taggtagatt ctctgctgct
    32041 gaagctcctg ctcctaaaaa tattgctatt ttcatatagt tcctcctaat ataaatatta
    32101 ctcctatttt tatatccatt aaatatccat atcatttatt ttatgataca acaataaagc
    32161 tgtcttttac tagaaaaaat cctattacaa gacagcctta tattatatta aatctttaat
    32221 aattcgtaaa tagaaagagc atatatttga gtagctttta taatatcctc tactattaag
    32281 tattcattag cttggtgttc tgtttctggt tttcctggaa aaatagctcc aaatgcaaca
    32341 caattatcta aagctcttgc gtatgttgct cctccagatg aaagtggtgt tccatcaaga
    32401 ccagtttctt cttcatatac ttttctaagt gtcttaacaa gaagagtatc ttctggaaca
    32461 tagattgaat ccaagaaatc atattcttca taatttaaat tgtatttatc tgtcattttc
    32521 tttaactctt ttacaaaatc atcctttttg tatgttactg gaatccttac atctattcct
    32581 gcaaattctt tttcgttatc aatagttact ctacctatat ttactgttag tttacctgat
    32641 acatcatcct tgcagttagg aattatattg tttccattag catcttcacc aataacttct
    32701 gctaaaaact taattatatt agaatctata cctatattat taagagctat acaaagtcta
    32761 gccactgcat ttataccagt atcacttgct gctgaatgga cactctttcc aattatacag
    32821 attttattac cttcaacagt atactcaaaa tttaatttat taagttcttt ttttaattta
    32881 tcagagtatt ttccaatata gattgcttta cctggaactg catttaaagc ctttccaact
    32941 gacaactcta tatcactttt gctatcacaa gtcaaatgaa cttgtaaaag acctttctct
    33001 gcatttgtta ttgggaatct agaatctgga gtaaatccat agtttggtat ttcttcatta
    33061 ttttctttat atttatttat acatctccaa agattttctt catcagcccc aaaaatgaat
    33121 cttatcctct tattaaagtc tacatttaaa tccataagag ctttcactgc ataaattgct
    33181 gatattgtag gtcctttatc atcttgtgtt cctcttccat acaatttacc atcttctaaa
    33241 acagcttcaa atggagggta attccagctt tctaaatcac cttctggaac cacatcaacg
    33301 tgaccaagta tacctatcat tttttcacct tgtcctattt ctgcatatcc ataatatcca
    33361 tctttgtata ctgtcttaaa tcctaaagat ttacataaat ctaatatacc tcttaaagct
    33421 ttatctacat tttcaccaaa tgggcaattt tcagttgctt cagatataac actaggtatt
    33481 tttacacttt cttgtattga agaaatcatt tcatcttgta gagaatttac tttttcttta
    33541 atttgctctt tcatattatc acccttcaat tttcatcata atatactaat aaatattttg
    33601 accttttaaa aaaaaaatat gcttattttt tcagtttttt tataaattca atcgctttta
    33661 aagtccccat ttttgaatgg tcaaatgtaa gtccaccttg gaaataaaca ttataaggag
    33721 gtcttattgg agcatctgca cttaattcta tagaagaacc ttgtataaat gctcctgctg
    33781 ccattatgac ttcactttcg tatccaggca tgtcccaagg aacaggtttt acataagaat
    33841 cgactggtgc agcttcttgt ataccttcac aaaaacttat tacttcatca gcattattaa
    33901 gtctaataca ttgaataata tcacttctta agtcatcata ttttggtaat acatcatatc
    33961 ctaatttttc aaaagctctt gagcaaaaga tagctcccat tacagcctga gatactatat
    34021 aaggcgccaa gaaaaaaccc tgaagtacat ttctagttgt tccaaaagta agaccacatt
    34081 cttttcctat tccaggagaa gtcattctat aagatataag ctcaatcaaa tcttttctac
    34141 cagctatata tccacctgtc aatgcaagcc caccaccagg attttttatt aaagagccag
    34201 ccataacatc tgctccaaca tcagtaggct ctttggtatc taaaaactca ccataacaat
    34261 tatcaaccat tactatagct tctggtttaa cagactttat tacttctata gcttctttta
    34321 tatcactaat tgaaagtgat tttctccaag aatatccttt tgacctttgt atcataacga
    34381 gtttagttct atcatttatt ttgtttttaa ttccttctaa atctaagttt ccatcctcta
    34441 aaaaatctac atcatcatat gtaacaccat actctttcaa tgaacctttt tcttctctta
    34501 tgcctataac tccctctaaa gtatcatatg gtctaccagt aacagataaa atttcatctc
    34561 ctggtctaac tataccttgt atacataaag taagtgcatg tgtaccattt acaattattg
    34621 gtcttacaag agcatcttct gtattaaata ctttagaata aatttcctct attttttcac
    34681 gaccaatatc attatatcca tatcctgtag tccagttaaa gtgcatatca cttagtttag
    34741 attcttgcat agcttttaaa actttatatt gattatattc tcttatttct tttatttctt
    34801 caaacttatc ttttatttct tccattattt cttgcgatag tttaaaagta tcatcatcta
    34861 ttccatagta gtcttttaat aattcttttg tctcattaag cattttatca cctgtctctt
    34921 tattatttta tttaaacatg taaaggagac tattttacta aaacaaataa attatagttt
    34981 ataagttagt ataagtctcc tctcaatcat cttaaattaa attaattatc tatctgttgt
    35041 tattattatt gttgttttgg ttttgatttg ttagattaat atactttcca ggttgtatag
    35101 ttgatacagc atgtttataa atcatgtttt gttgtctatt atccccttct attaatatta
    35161 tataactatc aaaccccttt acaagccctt taacttgcac tccattaact aaatatatag
    35221 taacaggtat cctttctttc cttgcattat ttaaaaacaa atcttgtaaa tttaaaactg
    35281 tatttttcat tagatttgta ccctcccaag attaaattga acttctaaaa tattcctacc
    35341 ttatattcta tataagttat gacaaatttt caatattaag ttgtatactt aaaaatttat
    35401 atattatttt atagaatctt taatatataa gattatttca ttttttaatt cgtctatatt
    35461 ttcatattgg tctaaatcaa accattttgc tgttttatat cttttaaacc atgttatctg
    35521 acgttttgca taccttctag aatctctttt aattatttca atagcttctt cgtatgtata
    35581 ttctccatcc agatacttta atatctcttt ataacctata ccttgcattg atatcatatc
    35641 ttttttaaat cccatactta aaagtttttt tacctcttca accaatccat ttttaagcat
    35701 aatatcaact ctcatattta ttctttgata caaatgttct ctatctctat ttagaactat
    35761 tattattggt tgatattctt cattaaattt taaatcactt gaaaaatcat tcattttctt
    35821 accacttaga caaacttcaa gagctcttat gaccctttta gtattgtttt tatggattct
    35881 attagcagat tcttcatcaa gttcttttaa cttattatgc atgtaatcta ttcccttttc
    35941 agcaagttgt ttttgaagtt cttctcttaa ttcattattg gcatcagatt gtgcaaaatc
    36001 catattatat attaaagagt ttaaataaag tcctgtgcct ccagttacta aaacacattt
    36061 ttccttttta tttatttcat gtatatattt ttttgctctt tgttgaaact ctgaaactga
    36121 aaatggatag tcaggcttaa cttcatctat aaggtaatgc ttcactcctt gcatctcttc
    36181 ctctgtaact tttgcacttc caatatccat atattcatat atttgcatag aatcagctga
    36241 aattatctct gcattcatat cttttgctaa ttttattgac aaatctgttt ttccaacagc
    36301 agttggacct gtaagtatta taagtggtat ttttttcata acattccttt ccatatattt
    36361 taaatatcca aatattttac ataattcttt taaacatttt ttctatttct gtcttagata
    36421 tttcgaccat tataggtctt ccatgaggac atgtaaatgg attctcacac ttttctagct
    36481 gttccaataa actttttatt tcaatatcgt atattttatc atttgctttt atagcagacc
    36541 tacatgccat agatgcaaat ctctctcctt ttaaatcata attacttgta atttcttcta
    36601 tattatcaat tatttgaagt ataaatttct cggtttcagg tactccaaat atagtaggaa
    36661 cacatcttac cattatatga ttattgccaa aaatttctag ctcaaaacca aatttcataa
    36721 ataactccaa gttattttca atttgaagca tatctacatt tgatacttca ataaccactg
    36781 ggtctaaaag tatttgcata tttatatctt gtctatagaa cttctccata tatctttcat
    36841 atagtattct ttcatgtgca gcatgttggt ccaacaaata catagaatcg tctttgctca
    36901 aaattatata agtattaaat acaactccaa ttacagaata tccgtataaa gaaaacttgc
    36961 gttttgaact acacaagtat tcttcttcac tgtccttaat actatctcct aagtaataat
    37021 ttccttcatt tttaattcca tcaacttgaa actcttcttg tatattatca tttgcactat
    37081 cttcacttag ataattaatg ttttttacat catcttctac acttgcattt aggacttcac
    37141 ttactgattg aaattcattt attggttttt cacttgataa agtaacaact tcaatgactt
    37201 catccttacc tttagtaata ttactatttt taggagtaga ttctaataaa tcattatttc
    37261 ttaatttata atctgtactt ttttctctac tgttaatagc gattctaggt tgtgtttttt
    37321 tatcactata agtagcatat tttccaataa gatttgagtg tattaattta acctttaaaa
    37381 aatctctaag ttctatatac acttcttgtt ctttttcaaa tttaatttca agtttattgg
    37441 ggtgtatatt tacatctata catgatgggt ctacttctat atttaagaag cacactgcat
    37501 gtttacctat tggaattata gacttataac tttctgttat tgcatctatg attattttac
    37561 tttttacaaa tcttttattt atatagatat gttgtaagtt tttatttgaa cggtatatat
    37621 tgttattacc tatatatcca ttcattttaa agtgattgca cttaaactct acatctatta
    37681 tattttccgt tatttctttt ccatatatac ttctaatagt gtttacaagc tttccatccc
    37741 ctggagtatt tagcatctgt ttattgttat ttgtatattt aaattgaatg tttggattac
    37801 ctattgcaag tttatttatc aaatcactta tatttatagt ttctgcatga gtagatttta
    37861 aaaatttctg tcttgctggt gtattaaaga aaatatcttt tataatgatg gtagttccat
    37921 tagtagaacc tattggttct ttcgatatta tttttccacc ttcaacatat attttagttc
    37981 ctattatttc atctttagtt ttagttgtca tttctaattt tgatacagct gaaatagatg
    38041 ccaatgcttc tcctctaaaa ccaagagagt ataaatcata caaatcatca atttttttaa
    38101 ttttactagt agcatgtctc aaaaatgatt tttcaacttc acttgaaggt attcctattc
    38161 cattatctgt aatcttaatt agactttttc ctccatcaat tatatctatg gaaattttat
    38221 ttgcacctgc atcaatggag ttttcaataa gttcttttac tacagaagaa ggtctttcta
    38281 caacttcacc tgctgctatc ttatttatag tcaaatcatc taggatgtta attatatttt
    38341 tcatttttac acctctaaga ttttatttct tttgcttttc tttgaagatt ataaagagag
    38401 tttatagcat ctaatggagt catatttaaa atatccagat ttaaaatttc ttctgatagt
    38461 atatctctat ttacactatc aaaagagatt tgagttaagg ctacttcttt tacagaatcc
    38521 acttgattaa ttttctcttt atcatcattt tgctttatta aagcaacttc atcatttaat
    38581 gctttaaatt tagaatttaa tttatcataa tcctttttta tatgtttgta gtcttttact
    38641 ataagttcat gttctatttt tagagattca tatttattct taaaattact ttgatttacc
    38701 ttaactaatt cttcgtccaa attactatta attatattat tgttttcttt atctccattt
    38761 attgcaacac tgttgtaaac atgatttttt tccaagtctt taagtatata ttttgctctt
    38821 tctatgactt cgtctggtaa tttagctaat ttagcaacat atattccata actcttgtct
    38881 gctccctgag gaattatttt tcttaaaaat attattcctt ccccatcttc tttgactgct
    38941 attgaataat ttttaacctc tttaaactct tcttctaaat cagtaagttc atgataatgt
    39001 gttgcaaata atgtcttaca tctaatattt ttttgaatat attccactat tgaccatgct
    39061 aaacttatac catcataggt actagttcca cgacctattt catctaatat aactaaactt
    39121 ctctctgttg cattttttaa tataagggat acttcgttca tctctaccat aaaagtagat
    39181 tgaccttgag ataaatcgtc actagctcca actcttgtaa atattctatc taatattgga
    39241 atatcagcag actctgctgg tacaaatgag cctatatgtg ccataagagc tattatagca
    39301 gtttgtctca tatatgttga tttacctgac atattaggac cagttattat gttaataatg
    39361 ttttctccac gatttaaata agtatcattt ggaacgaaat tttcctctcc tactatattt
    39421 tcgacaacag gatgtctacc atttcttata tcaagtttat tattttcatt tattgctggt
    39481 tttacatagt tatttatatg tgcaactgtt gcaagggaca caaaaacatc tatattagct
    39541 atagtcttag ccactttttg tattctatct atgtttttat atattgtatc tcttatctct
    39601 acaaatatct cgtattctaa agatttaatc ttttcctctg catgtagtat cttttcttct
    39661 atttccttta attctggagt tatatatctt tctgcattac taagtgtttg ctttcttata
    39721 tatgtctcat ctagctttgc ctgtttaaag ttagcttttg ttatttctat ataatatcca
    39781 aaaactttat taaagcctat ttttaatgat ttaactcctg ttttttctct ttccctgttt
    39841 tctatttcct ttacaagaaa ggctccattc ttagaaatct ctcttagttc cttaagttca
    39901 tcactaaaat ctgatttaat tatgtttcca tcttttatgg ttatcgtagg ttcttctaga
    39961 atagcttcat ctattaaatt gtatatatca tctaatttat ccatttctga aacatactct
    40021 tttaggattt ttgcatttga taaatttata gtatctttta aattaggtaa cttttctatc
    40081 gaatttttta agtgaattaa ctcttttggt gttactctct caaatgctat ctttccacat
    40141 attctttcta tatcgtatat atttttaagt atatcattta ggtcttctct taatataaag
    40201 tcatctttta tttcttctat tacatctaat ctattttcaa ttttagattt atttatcaaa
    40261 ggttcttcta catattttct taaaagtcta cctcccattg ctgtagaagt tttatcaagt
    40321 acgtgtaata atgaaccttt tttcttattc cctcttatag tttgagtaag ttctaaatta
    40381 gttctagtaa acatatctag aaccatatat tctgatgaat tgtatatatt tatattatta
    40441 atgttagatg ttatttgttt ttgagtattg tatatataat tcagaagaat tgataagctt
    40501 gactttatta gacctttatc atcaaacttt agtttttgta gataaacatc tggaaaatat
    40561 tcttttaata tatttatatc caaatagtta tcactaaaac tttcatttat gtatatatta
    40621 cttacagtag ctatatctct aagattttct ataaagtcta aatcatttat aattatttca
    40681 gttggatgga tttttgcaat ttcttcaatt accttatctt catttaaaca tgttgcatta
    40741 gtttctcctg tacttatatc aacataagtt agacctatat tagtaccatc cttatacaaa
    40801 gataataaat aattattctt tttattttca agtaaatttc catctaaaac tgtaccagga
    40861 gtgatgactc ttataacctc tcttctaaca attccttttg ctgtagatgg gtcttccatt
    40921 tgttcaccta ttgctacttt atatccattt tcaactaatt ttgatatata tgaattggct
    40981 gagtggaaag gaacaccaca cataggagca cgttcttcta aaccacaagc ttttcctgtc
    41041 aatgctattt caagagcttt tgaagctact aatgcatctt caaaaaacat ttcataaaaa
    41101 tctccaagtc taaaaaacag tatacagtct ttatatctat tttttacttc aagatactgt
    41161 ttcatcatag gagttaattt attcatatct atttgcatta tcaaacctcc ttcttttcta
    41221 attaaatatt atatcatagt agatgattat tattaacaca atatataaaa ttttgacata
    41281 taactaaaaa caatccaaaa tgtatactca cattttggat tgctaagttt aatgcttatt
    41341 aaataaaaat atttttttta caataaagat attatttctt ttttaagttt aatgatattt
    41401 tcaaggttat cttcatttaa ctttctacta tctataacaa attctttctt tactattgat
    41461 ggtttttttg aaataacata aattctgttt gatagcatta tagcttcgtc aatatcatga
    41521 gttataagaa gtattgttga atttactttt ttcttaacat ctaaaagcca tttttgcatg
    41581 gatgttttag ttaatgaatc aagtgcccca aaaggctcat ctaaaagcat tatgtcatta
    41641 gaatttataa aagttctcaa gaagttagct ctctgtctca ttcctccaga aagttctgaa
    41701 ggatatttat cttcatatcc gcttaatcca aacacctcaa aataaggttt taccattgac
    41761 ctagctttac tctttttttc tttttttaaa attagtggta gtgacacatt atctattata
    41821 gttttatatg gcaagagtaa atctttttga tacatataac tatacttacc atttatattc
    41881 actttgccac tatcataatc tgtaagttta gttataatat taaaaatagt actttttcca
    41941 catccacttg gccctaatat agaaacaaac tcaccttcat aaacatcaat acttatatct
    42001 tttaaaactt caatatcttt ataagttttt gatacatttt ctatagatac ttttactttt
    42061 ttattgtggt aaaaattcat ttgtgtaagc atcacttgcc ttcatatcct tatctattaa
    42121 tttatattct tttaagaatg atgtatagtt gttccataca ctatctttca tttctcccca
    42181 tcgtggtgca tcatctttgt acttgcttgc taaatattct tgactcttta aagctagttt
    42241 ttcatctact tcaggtgcat gttttactaa aatttttgct gattcttctg gattttttat
    42301 tgcatattca taaccttttg ttgtagcttt taagaatttt ttagcaagct ctggattatc
    42361 tttaagcaca gtttcattag aaattataag tggtgtataa taatccaatc tctcatctaa
    42421 atcttttact ggtataaagt ttaagtcata tccaattaaa tcagctttaa ctgcatccca
    42481 accttcaaat atccatgcaa aatctacagt tttcatagct gcaaagaaat catcttgacc
    42541 agtattaact atctttaact tattaaaatc agctccattt ttcttcataa ctgctttaaa
    42601 aacagcttct tctgatggag agccccaacc accataagtt tttccttcaa aatctttagc
    42661 tgttgttata ttcttttctt ttggtgatgc aaacccagaa gtattatgtt gtataacagt
    42721 tgctacagct tttattggaa gtggatcttc actagtcttt gcataagtaa cttgttcttg
    42781 atagcttata ccaaagtcag cttttcctgt tgctactaaa gtttctgctc cactttcagg
    42841 tggttgtact atttcaacat ccaaaccttc ttctttatag taacccttat caagtgctac
    42901 aaataatcca gtatgatttg tatttggtgt ccaatcaagc accatagtta cttttttaga
    42961 agaagtatct ttatcattgt ttttgtcttt attgttattt gaagaacatc cagttattgt
    43021 tgccaatgta aaaacactta ctaaacctaa tattcctaac tttttaaaat tcataattgc
    43081 ctccctcaaa tacctttaat ctattaatct atgtcttata acaacttttt ttacagcctg
    43141 tacaattcct acaaacatta aactaaatat aactactaat atagtagatg caaatacttt
    43201 atcaagtgca tatgcacttt ttgacctaag catataaaca ccaagtcctt tcgttccacc
    43261 tagccattct gcaacagttg ctgccattac agcatatgtt gcagatattt taagtccaga
    43321 aaaaaattta tccattgcca taggaaattt aaggtgaatg aatgtattaa ctttatttga
    43381 gtccatagtc ttaaataaat ttaaataatc tttatcaata ttctcaatgc catctacaaa
    43441 acttattaat ataggaaaaa aacaagtaag cattaccatc aatacttttg gcattgttcc
    43501 aaatccaaac catatcataa agagtggtgc tataattatt gtaggtatca tctgtgtaac
    43561 caacataata ggataaagac atttttttat tattgataca aaatccatca atattccaat
    43621 taccaatgat attataattg cgactataaa accaagtatt gcttctccta tagtaaccac
    43681 agcatgcatg gataaatttt gataatcttt aaaaaacaca ttaattatat ctactggtgt
    43741 gggtagtata tactgaggaa cttctaaaac tcctaccata gattgccaaa gaataagtat
    43801 aaacaaaaat gttgctattg gatatatact atctttgact ttatctatga tttctaagtt
    43861 tttcatctat agtcagacct ccagcttttt catctactct agctactgtt atgactcttt
    43921 cacatccaag attaaaaggg attttatgta ccttttttaa taattcaaaa acttcatcaa
    43981 aatcaccttc aacagtagtg ctcattgctc ctatttcaca tttaagacca cttttttcaa
    44041 tttcagcaat acaagcatct actactttat acatctcttc ttcacctttg taaggtctta
    44101 gtggcattac tgctatgtct gcgattacca tttaaatcac tcctttaaaa ataattaata
    44161 cctactaggt atattttgtt ttttattaaa aaattttatt actaaaaaga tattatattt
    44221 aaataaaaga aaagtcttat ccaaaatagg ataagactta tttttatctt aactggtctc
    44281 cctacactgg cattatccaa acaggttcaa agggtcgaga taaaatctcc tctcagcaca
    44341 ttagctcccc gaaatatatt atatcttttc tacttgatta tactacactt atcatatttt
    44401 aacaaatttt ttatttatat tagatatttt atccgttcaa aaatatctaa tataaattgt
    44461 acattattac accaaataaa ttatcttaaa atggtatttt atcaacatct tccaaaacta
    44521 gattaactga attttctagt ttttattttg ctatttcaag catatttgga tgtatatcaa
    44581 ttccatacaa atattgtgct aaatttctgt tttcactcat ctaatttgca atcgttacag
    44641 attacagatt agaaacagtt tttagactct acttatcttt atctacaaaa aatctttcaa
    44701 taaattcatt aataatattc agtaatctaa aaataaacct ctatctggat ttttaagata
    44761 caaacctttt aggaagtatc ttttgtgata atataaaaat tactcgtcat tatcatc
//
LOCUS       DAYATN010000017        43963 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.017, whole genome shotgun sequence.
ACCESSION   DAYATN010000017 DAYATN010000000
VERSION     DAYATN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.017"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            184..534
                     /locus_tag="ACGI8A_000939"
     CDS             184..534
                     /locus_tag="ACGI8A_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFH0178947.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            553..2196
                     /locus_tag="ACGI8A_000940"
     CDS             553..2196
                     /locus_tag="ACGI8A_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFH0178948.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2277..4334
                     /gene="recG"
                     /locus_tag="ACGI8A_000941"
     CDS             2277..4334
                     /gene="recG"
                     /locus_tag="ACGI8A_000941"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFH0178949.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4393..4947
                     /gene="rsmD"
                     /locus_tag="ACGI8A_000942"
     CDS             4393..4947
                     /gene="rsmD"
                     /locus_tag="ACGI8A_000942"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFH0178950.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4949..5446
                     /gene="coaD"
                     /locus_tag="ACGI8A_000943"
     CDS             4949..5446
                     /gene="coaD"
                     /locus_tag="ACGI8A_000943"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFH0178951.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5450..5974
                     /locus_tag="ACGI8A_000944"
     CDS             5450..5974
                     /locus_tag="ACGI8A_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178952.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6318..8237
                     /locus_tag="ACGI8A_000945"
     CDS             6318..8237
                     /locus_tag="ACGI8A_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0178953.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8250..8537
                     /locus_tag="ACGI8A_000946"
     CDS             8250..8537
                     /locus_tag="ACGI8A_000946"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0178954.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8670..9929
                     /locus_tag="ACGI8A_000947"
     CDS             8670..9929
                     /locus_tag="ACGI8A_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFH0178955.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9932..10672
                     /locus_tag="ACGI8A_000948"
     CDS             9932..10672
                     /locus_tag="ACGI8A_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFH0178956.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10995..12305
                     /locus_tag="ACGI8A_000949"
     CDS             10995..12305
                     /locus_tag="ACGI8A_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFH0178957.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLISS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12403..13320
                     /locus_tag="ACGI8A_000950"
     CDS             12403..13320
                     /locus_tag="ACGI8A_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFH0178958.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13336..14145
                     /locus_tag="ACGI8A_000951"
     CDS             13336..14145
                     /locus_tag="ACGI8A_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFH0178959.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14203..15951
                     /locus_tag="ACGI8A_000952"
     CDS             14203..15951
                     /locus_tag="ACGI8A_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HFH0178960.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKVREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     RYDKVKKYVLELLDRV"
     gene            16255..17235
                     /locus_tag="ACGI8A_000953"
     CDS             16255..17235
                     /locus_tag="ACGI8A_000953"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HFH0178961.1"
                     /translation="MFFLSISFIGFYLVIRDNSDTVKMQRGINIGNALESPKDFPWDV
                     KMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKIDKYVDYALDKDLIV
                     VLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELLNEPKENLSSQLLNE
                     YIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVVSFHYYEPNDFAFQG
                     NIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIFLGEFGITKEAPEVS
                     RRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILSALIEKR"
     gene            17240..19546
                     /locus_tag="ACGI8A_000954"
     CDS             17240..19546
                     /locus_tag="ACGI8A_000954"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0178962.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTTS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            19557..20633
                     /locus_tag="ACGI8A_000955"
     CDS             19557..20633
                     /locus_tag="ACGI8A_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178963.1"
                     /translation="MKTLKVFFCSTKYLILVFIFFINTTFISSATSDKILDLSFKKIE
                     TDLNSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNFYIEIQNKNKSSQR
                     INLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEFEGKIYVNFNSLIN
                     EESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKLRFLNNIKIIGDEE
                     VQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTLSNKAKPGQIILQV
                     NVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMKKINFMNNIITFVR
                     ILFVSLVIICFGIYLYWKKCSKTK"
     gene            20651..22069
                     /locus_tag="ACGI8A_000956"
     CDS             20651..22069
                     /locus_tag="ACGI8A_000956"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HFH0178964.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22084..23292
                     /locus_tag="ACGI8A_000957"
     CDS             22084..23292
                     /locus_tag="ACGI8A_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178965.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23366..24754)
                     /locus_tag="ACGI8A_000958"
     CDS             complement(23366..24754)
                     /locus_tag="ACGI8A_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFH0178966.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25095..26429
                     /locus_tag="ACGI8A_000959"
     CDS             25095..26429
                     /locus_tag="ACGI8A_000959"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HFH0178967.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26571..27488)
                     /locus_tag="ACGI8A_000960"
     CDS             complement(26571..27488)
                     /locus_tag="ACGI8A_000960"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFH0178968.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27696..28580
                     /locus_tag="ACGI8A_000961"
     CDS             27696..28580
                     /locus_tag="ACGI8A_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HFH0178969.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28793..30634
                     /locus_tag="ACGI8A_000962"
     CDS             28793..30634
                     /locus_tag="ACGI8A_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFH0178970.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVED
                     IKIENKSARLIGKEEALKYKLDSVVSKKVDKIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSSRESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            30939..31646
                     /locus_tag="ACGI8A_000963"
     CDS             30939..31646
                     /locus_tag="ACGI8A_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0178971.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            31640..32950
                     /locus_tag="ACGI8A_000964"
     CDS             31640..32950
                     /locus_tag="ACGI8A_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0178972.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNVAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKSEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            33067..33750
                     /locus_tag="ACGI8A_000965"
     CDS             33067..33750
                     /locus_tag="ACGI8A_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0178973.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDEPFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            33743..36130
                     /locus_tag="ACGI8A_000966"
     CDS             33743..36130
                     /locus_tag="ACGI8A_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0178974.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            36410..37552
                     /locus_tag="ACGI8A_000967"
     CDS             36410..37552
                     /locus_tag="ACGI8A_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HFH0178975.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            37571..38095
                     /locus_tag="ACGI8A_000968"
     CDS             37571..38095
                     /locus_tag="ACGI8A_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFH0178976.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            38195..39013
                     /locus_tag="ACGI8A_000969"
     CDS             38195..39013
                     /locus_tag="ACGI8A_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HFH0178977.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            39091..39402
                     /locus_tag="ACGI8A_000970"
     CDS             39091..39402
                     /locus_tag="ACGI8A_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFH0178978.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(39579..40262)
                     /locus_tag="ACGI8A_000971"
     CDS             complement(39579..40262)
                     /locus_tag="ACGI8A_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178979.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(40355..41005)
                     /locus_tag="ACGI8A_000972"
     CDS             complement(40355..41005)
                     /locus_tag="ACGI8A_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0178980.1"
                     /translation="MSFLGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDG
                     LSRGAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHTYEQHN
                     KQDIFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMF
                     GFLLNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(41170..43173)
                     /locus_tag="ACGI8A_000973"
     CDS             complement(41170..43173)
                     /locus_tag="ACGI8A_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFH0178981.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(43326..>43963)
                     /gene="recX"
                     /locus_tag="ACGI8A_000974"
     CDS             complement(43326..>43963)
                     /gene="recX"
                     /locus_tag="ACGI8A_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFH0178982.1"
                     /translation="GIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNEI
                     NEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKSY
                     NLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENAM
                     YLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        17112 a         4805 c         7552 g        14494 t
ORIGIN      
        1 aattattatt tttctttgaa tatatatact atagaagaaa ttttatccaa aaacttttct
       61 aggtttaaat tataccatat ttatagtatg atatgattat aattttatat aataaataag
      121 taaaaatagt aaaaaataaa ttaagaaatg tttttaataa aaataagctt aagggggtca
      181 accatgagtg caaaggtaac gaatcaatat ggaaatatag aaatagataa tcaagtaata
      241 gctcaagtcg tatatagagc tgcaatggaa agttatggct tagtaggttt agcatataag
      301 tctaaaggaa tagtagagct attaaaaggc gagaacgcaa ctaaaggtgt aagagttgag
      361 gaagtagaag aagatgccat agcaatcgaa ctttatgtta taatacaata tggtacaaat
      421 atatctactg ttgccaataa tataatagat agagtaaaat atgtagttga aaaaatgact
      481 ggcgtaagag ttactagaat tgatattaat gtacaaggaa taagagttaa gtaatttcat
      541 aggaggaaac aaatgattca atatatagat ggtaaaaggt taagggagat gtttatttca
      601 ggtgcaaaca atctccaaaa caataaagag ttagttgaca agttaaatgt attccctgta
      661 ccagatggag atacaggaac taatatgtca ttaacaatat cttatgcctt aaaagaatta
      721 gcaaaagttg aaaatgataa tatatcagat atcggaaaag cattatctaa aggttcttta
      781 atgggagcta gaggaaactc aggagttata ctttcacaaa taatcagagg tatagctaaa
      841 tctatagaag gaaaaagtaa attatcaaca gaagatttag caaaagcttt taaaaatggt
      901 tcagacacag catataaagc tgtaataaaa ccaattgaag gaactatact tactgtagtt
      961 agagaaagtg gagagtttgc aataaaaact gcgaaaaaag agaaagatgt agtaaagttt
     1021 ttaagtatgc ttgtgaagga atcaaattct tcattagaaa gaactccaga ccttctaaag
     1081 aatctaaaag aagctggtgt ggttgactct ggtggaaagg gtcttgtact tatatatgaa
     1141 ggaatgttag cttctataaa aggcgataac atagaaataa aaaatgttga cctagatact
     1201 aatatttcaa caagtatgga ttttgcaaag agtactacta gcacagatga tataaaatat
     1261 tgctattgta cagaatttat tttagaaagt tctaaggtag aagatactaa aataagagac
     1321 ataatgatgg cttatggtga tagcctagca gtagttggag atgatggagt cataaaggta
     1381 catgtacaca ctaatgaccc aggaaatgta cttcaagaag ctctaaagta cggtcaatta
     1441 ttaactataa aaatagaaaa tatgaagtta caacatgaaa atacattact ggatgtagaa
     1501 gaaaagaaag aaaatgatag tgaaccttta gaagaagaaa aagagtttgg gtttatagct
     1561 acttctatgg gtgaaggttt ggcaaatata tttaaagatt tcggagttga ccatataata
     1621 gaaggtggtc aaactatgaa tccaagtact gaagatttta tgaatgctat aaagcatata
     1681 aatgctaaaa atatatttat tttcccaaat aacagcaata taataatggc tgctaatcag
     1741 gcaaaagaat tgagtgataa aaatataata gttataccta caaaaaatac gcctcaagga
     1801 tttgctgcat tagtaacttt taatggagaa ttaagcgaag atgaaaataa agaagctatg
     1861 atgaatgctt taaactcagt taaatcaggt caagtaactt ttgctgttag ggatacagtg
     1921 atgaatgaaa ttgatgttaa agaaggtaat ataattggaa ttgcagaagg aaatttatta
     1981 tcagcaggag attatgtgga tgaagtaaca tcaaacctta ttgaaaagtt ggttgatgaa
     2041 gatactgcta taataacatt attctttggt gaagatgtta cagaatctca agcaaatgaa
     2101 ctacgtactt ctttagaaga aaagtttgaa gatgtagatg tagagttata ttatggagga
     2161 caacctcttt attattactt aatttcagtt gaataatgat tataagaacc tgtatgacca
     2221 agtagctttt ataaaattgg ttgtacagga tttttgtgta gtaggtgata tatttgttgg
     2281 atttatataa agatgttcaa tatgttaagg gaataggacc taaaaaagca gataaattaa
     2341 ataaactggg tatatttaca ctaaaagacc tattgtatta ttttccaaga caatttgaag
     2401 atagaaataa tctaaagaaa atagcacaat tagaagatgg tgagaaagtt acgataaaag
     2461 cagtaatatc aagtataaat acatttagcc caaaagaagg tatgacttta acaaagattg
     2521 atgtaaaaga tgagacaggc tcggctaaat tagtgttttt taataagagt tatataaaaa
     2581 atacttttcg tccaggagat tcaatattag tttttgggaa ggttaagaaa aaatttaata
     2641 acttagaact tacatcatgt gaattagaat atcttactaa ttcacctaag aatacatgta
     2701 gatttatgcc tgtgtaccag cttacatacg gtgttacaaa taaagaaatt atgagtataa
     2761 ttagaacagt acttgaagat aaagaattaa ttatacaaga atatatgcca caaagaatta
     2821 tagaaaaata taggctatgt agtatagatt ttgcagttag aaacatacat tcaccaagta
     2881 gtaaggaatc attaaaaata gctttatata gaattgtatt tgaagaatta cttatactac
     2941 aactagggtt gtttgtattt aaaagtggca gaaataaaga agatggaata aagtttgaaa
     3001 caagtaaaga cttaaaaaaa ataataagtg ctttaccatt taaactgact aaagctcaaa
     3061 acagggcatt agatgaaatt atacaagata tgaatttaga gaaaataatg aatagattgg
     3121 tacaaggtga tgtagggagt ggtaaaacag tagtagctct cttagcactt gcaaattgtg
     3181 ttttaaatgg ttatcagggc gctttgatgg cacctacaga gatattagca gggcaacatt
     3241 atatttcact tactgagtct ctaaaagatt ttggaataaa tgtaggtcta ttaataggta
     3301 gtcttacaaa aaaacaaaag gatacagtat tggaacaaat aaagaataat gagattgata
     3361 tattaatagg tacacatgca ctaattgaag ataaggttga atttaataat attggtttag
     3421 ttataacaga tgaacaacat agatttggtg ttatgcaaag gagcaaactt tcattgaaag
     3481 gagcaaatcc tgatatatta gtaatgacag caacaccaat accaagaact ttagcactta
     3541 tactctatgg agatttagat atttctatta tagatgaact tcctccagga agacagccaa
     3601 tagaaacaat tgctattgaa aaaagtaagc gagatagagc atataataat cttgtaagaa
     3661 gagaggttga atcaggaaga caagtgtaca ttgtatgtcc cttggtagaa gaaagtgaag
     3721 ccatagaagc caaatcagca gttgaacttg ttgaggagtt aagagcggaa tattttcacg
     3781 acttaaggct tgggttgctt catggtaaaa tgaagtcaag tgaaaaagat gaagtaatga
     3841 gactttttaa agataaagaa atagatattt tagtttcaac aacagtaata gaagttggtg
     3901 tcaatgtgcc aaatgcaaca ttaatgataa tagaaaatgc agaaagattt ggacttgcac
     3961 aattacatca gcttagaggg cgagttggaa gaggtagtca taaatcgtat tgtgtactaa
     4021 tttatgattc taaaacagat gtatgtagac aaagaatggc tataatggaa gagaccaatg
     4081 atggttttaa gatttcagag aaggatttag aaataagagg acctggggaa ttttttggga
     4141 caaggcagca tggtcttcca gaacttaagg tagcaaactt atttaagcat ataaaaatac
     4201 tgaagttagc acagcaagaa gctcgatata tattgggtga agataataat ctacaattaa
     4261 aagaaaacat ggcactcaaa aaagaaatta tagataaatt taaagataca ttgaaagaaa
     4321 tttcattaaa ttaattttaa attgtgttaa aatattacta atgtaataat gtgctgaagg
     4381 gagattgtca tattgagagt aatttcagga aaagcgagag gattaaaact aaatactcca
     4441 aaaaatgaag atgtaaggcc aactacagat agagtaaaag agtctctatt taatatgata
     4501 aattcatata taatggaaag tgaagttctg gatttatttg ctgggactgg ttctttagga
     4561 atagagtgct tatctagggg tgccaagtca tgtacttttg tagatattag taaggaaagc
     4621 atagatattg ttaagtccaa tataaaaaag gcaagagttg aaagtgaaag tattatttta
     4681 aaccttgatt ttaagactgc aatagataaa cttaaattac aaaatagtaa atttgacata
     4741 atttttatgg accctccgta ttataaaaat atgtttatag aagctattga aaaaattgat
     4801 aattctaatc tattgaacga agatggaata atagttgtag aacatgatac gaatgattta
     4861 tttccagata agatatgtaa attggagaaa actagagata agaaatatgg aaatactaca
     4921 ttaacttttt ataagatgga ggcataatat ggaaaataaa cctagaaaag ctatatttgc
     4981 aggaagtttt gacccaatta ctaatggaca tttagatatt atttgcaggg cttcgaagtt
     5041 atttgacgaa ttacaaatag gagtattaaa taatcctaat aaaaagggtc tatttagctt
     5101 tgatgaaaga gtaaaactta tagaaaaaag tacaagccat ttaaataata taaaagttgt
     5161 gacttttgat ggtttattaa taaattactg tcaggaaaat gggattggag cattagttag
     5221 aggtgtgaga agtggtgctg atgttgatta tgaacttcag atggcacata tgaatagaga
     5281 gcttaatcca gatatagaga ctataatttt accaagttgt acaaaatatt catttataag
     5341 ttcatcttta ataaaagagg ttttgctctt tgatgctgat ataaaaaatt tggttccaaa
     5401 gattgttttg gaagaattaa agaaaaaaac tagtgggggt aattgatata tgaagataga
     5461 tttagaaatg attgaactat ttgagcagtt agaggagata tttagaagtg cttctacaat
     5521 ccctttctca cataaatgca cagtggataa agatgaggtt ttagcaataa ttaatgatat
     5581 aagaacttta ataccagaag aagtaacaca ggcagtatgg attaataaag aaagaaataa
     5641 aataataagt caagctaagc aagatgctac aaatatagtt gaacaagcga aaaaagaagc
     5701 tgagagaata caacaagaat atcaagataa tatagaggaa ttaaagaaaa attctgaaga
     5761 tgtagtaaag gcatatgtag aatccagtga acctgttgtt caagcagacc aaagagcaaa
     5821 agatatagtt gatagagctg agagaatagc caatgagata agaattgggt caattgaata
     5881 tgcagaggat gtgctttcta gtgttgagta taatttaaga gaaatattag aagaagtaaa
     5941 aagagataaa gctgaactta gacctaaaaa ataaatttta aataaataaa aatggagcta
     6001 tcacacaaaa aattccaatt tatgtgtgat agctctcttt taatatagca attttttgtt
     6061 atataattgt gctttttaga ttacttatat cttattttga tattataatg tagtttaatc
     6121 ttttatttgt tgaaatcaac ttatttattt gtataaataa tataggttat tttcaaattt
     6181 ttttaataat tttaccactg tcttctttta gattagagtt agactgtgca aaattataaa
     6241 ataatactaa atcatgtgca attatttggt atggcactac atgtagaaaa tatagataat
     6301 aaaattaagg aggtagtatg aaggaaaggg ctaaaaaaat attagatttt attattaaaa
     6361 ataacaatgt aacatcacaa gaactacaag aaaagtttaa tgtttcaaag agaactattt
     6421 attacgatat tctagctata aatgaacaac taggaaaaag tggtaatata aaaaatgtaa
     6481 aacacaaatt tatatttgaa ggtaatttat gtgatgcaag gaaaataatt tcaactgaag
     6541 aagataaatt tttagatagt gattacagaa aaacttttat attaaacaag atattacttg
     6601 gcgaaaaaat atcaatagaa aaactaacaa atgaaatgtt actttcaaaa aacacagtag
     6661 tacaaacaat aactgatgta aaaaaatacc ttcaaacaat ggggttaaga ttagagtata
     6721 agggaaagta caaaattata ggtgatgaat acgttattag agagctgttt ctgataattg
     6781 ttcaagaaaa tatattggaa attaattcta taagtgagga agtaagtagt ttcgatacaa
     6841 aaggacatat aaaacttact gattattctt tattaaatct aactaagttt gtggaatttc
     6901 taaataaaag gataagagat gggaaaacat tatactcata taaatatcta aatgaagcta
     6961 aaaaaattag ttactttagt aattgtaaag aacttttatg tgaagaagct aatgaaaatg
     7021 aacaagccta tatatgcact tacataagtt cacttcccag cttaaatagt gaagttaagg
     7081 aagatgttgt tgaagaatat gtagataaac ttatagataa gtttgaagta aatacagcaa
     7141 taaaattaga aagtaagcat gagtttaaga aaaatatttt aaggcacctc cattcttctt
     7201 acaacagaat acgatttaag tttcctatta gaaatccaat gttagatgag actaagtata
     7261 aacatgagtc actttataaa attataaaat caattataga aaatgaagaa gagtttcctg
     7321 tatttgaggg gataagagaa gaagaaatag gatttatagc agcgtatttt ggaggatatt
     7381 taagagggtc tagggataat ggtctaagaa gaaataaagt cttactagtt tgtcctaatg
     7441 gtcttatggt atcaaagagt ttggaaatac aactatataa atacatacca accatagaaa
     7501 tagtaggtat agtatccatt aagcaactaa aagaagtaaa tgtatactac gattacataa
     7561 ttacaactat agatatacaa aatgtaaaca acgtaattgt tgtaaatcct cttttaacat
     7621 catcagatgt tcaattactt atgaacaaac taataagtgt aaaagaaaac gagaagtatt
     7681 tcaatcttga attaataata caagctataa gaaaaaatgg agttatcaat aatgaagaag
     7741 cactaaaagc tgatttatta aacataatac acaagataga tgaaggagag atgtatcaac
     7801 caatgttaaa ggaattaatt aatgcagaaa gggtcaatat aataaaaagt gttagagatt
     7861 ggaaagaagc aataaaaata gcctcaaagc cattgttaga agataactct atagaagagt
     7921 tatatataga aaatatgata aagtcagttg agaaatatgg accatatatt gttttagctg
     7981 atagatttgc tctaccacat gcaagttcta aagaaggagt aaacaagctt gctatgtctt
     8041 tactaatagt agaagatgaa gttgatttat taggaaaacc agtaaatata tttatggtat
     8101 tagctgctgt agataacact actcacatta gagcattagc atcactatca gagatgatgt
     8161 acgaggaaga aaatgtaaaa ctaataatta atggtgataa aagttcaata ctagaactta
     8221 taaataagca aaattaggag gaatgaaaaa tgaagatatt agcagtatgt ggttttggtg
     8281 taggttcatc aatggtatta aaaatgacaa tacaaaaggc tctaaagcag ttaggtatag
     8341 aatgtgaagt agaaaataca gatataaatt cagctagagg tgctgattgt gactgtatat
     8401 tcacaagtca agaattgtta aatgaattaa aaggaacttg taatgtagat gtatatccag
     8461 ttaaaaagta catggattta gcagaagtca cagaagttgt tcaaactttt ttaagtgata
     8521 aaaatgataa gaaatagtat aagttttaaa aagtaaattt aagttaatca gaataaaata
     8581 aaaacttact aaattcaaaa atagtaagaa agagaggagg gatttagtgg tagaaaactt
     8641 aaacagttga cctatcacta aaaaatataa tgcttgattt tatttcaaat aatattttac
     8701 gtaatccatc tatgttaatt ggattaatag cagcatttgg tcttgtacta cagaagaaga
     8761 aagcatctga tgttataaag gggtcactac ttgcagcttt cggaattata attatggaga
     8821 ctggaacagg tatgttagtt agttcaatag caccaattaa tggagctttc caaagtataa
     8881 gtggtggtgc agtaacaaaa ggtttaagtg atgttacttt tactgcaagt tatggtggaa
     8941 ttataggttt agcaatgttt gcgggtttag ctattcacct aatgatagca agatttactc
     9001 ctattaagac aatcttttta acaggtcaca tgctttattg gttcccattc atatttgtag
     9061 cttcaggagt tgaagctgga ttaaatggtg gagtattaat agcttttgca gcagtattat
     9121 cagcactcta ttggtcaata atgccttggt tattaagaaa atatgtgttt gatgttatag
     9181 gagatgattc ttttacatta ggtcatccat ctggttgttt agcacttata tcaggatttg
     9241 ttgctaaatt agtaggaaat aaagaaaaat ctacagaaga tataaacata cctcaaagtt
     9301 tatcattttt tagagaaata tctataagtg gtggaatggc agtgttttta atgtttatag
     9361 ttgttgggat agtaatacca ggtgcgttta aagatagtaa tgagtttatt ggaacgtatg
     9421 caataaagca aggtctttct tttggagcag gtcttataat aatgcttcat ggggtaagaa
     9481 tgctaataaa tcaaataatg ccagcatttc aaggtatatc tgaaaaagtt gtgccaaatg
     9541 cactacctgc ttttgactgt ccaatattat ttaattataa accaaatgct gtattaatgg
     9601 gattcttagt tgctatgcta acatctacag ctatgatttt aatatgtaac tactttaatg
     9661 tatttaattt gctacttata ccacttgtta taacttcatt ctttgagtgt ggagcagcag
     9721 cagtaatagg agaatcacaa ggtggtatac gtggagctat aataggtaca gctatagctg
     9781 caatatttat ggtagtttta gtaggaatat ctgctgtaat ttactcagga actattcaaa
     9841 attggatact aatatttgga ggtaatgacc tttcattatt tggtataatc agtaggttta
     9901 taggttcact atttggagga atattataga tatgaaattt aaatacatag atgagataag
     9961 aagattaatt gatataatag aaaacgaaga aaaagtcaat atggaaaaag cagtacaaat
    10021 ctttgttgaa gcaatagaaa gtaaaaatgc tatattttca tttggagctt cacatgcagg
    10081 aatattaaca gaagaattat tctatagagc aggtggctgt gttcttataa atccagtatt
    10141 tgcaaaatct ctaatgttag atacagcgcc tataactcac acaagtaaga tggaaagatt
    10201 agaaggatat ggtacagctc tagctgataa aactccaatt aaaaaaggag atgtagttat
    10261 agttcattca gtatctggta gaaatccagt aagtatagag tttgctatga aagctaagga
    10321 aaataaggca atagtaatat gtataactaa tttaagttac tcaaaaagtg tatcttcaag
    10381 acattcttca ggaaaaaaac tttatgaagt atgcgattta gtgatagata atcatggtca
    10441 aaagggagat gcctgtgtag agatagatgg attaaaccaa aaagttagtc caacttctac
    10501 tgtaatagca actactgtga tgaactctat tatagctcaa gctactcagg aattagttaa
    10561 taaagggcta aagaatcctc cgattttcta tagtgcaaat attgatggtg gagatgagtt
    10621 aaataagaag atttttgatg aatataaaag tgtaattcat tatgaatatt aaggttaaaa
    10681 aaatatgtta atgaactttg aagtttatca acaaagaata ttaataatat ataataaatg
    10741 ctatcattta aaagctgtag gtaaaaatct acagcttttt attattgtag attaaaaaga
    10801 tgcgtaatta aattttaaat tggagaaaat aaattggaag gtatcaatta taaatattag
    10861 aaacgttgtc atagaaaaaa actatattaa aaaaaagtac atagaaaaaa ctgattagac
    10921 aataaataca tgctttgata atattttaaa tggagtgaaa atgtgtagaa ttataatgaa
    10981 aaaagaaaat aaaaatgaaa aaagaaaata aaatggaggg aataaaagtg ggtatgttaa
    11041 aaaaattatg ttcactagct atggtagcaa ttatgactac aacacttata acagggtgtt
    11101 caagtggaaa tggaaaagat tcaaaaaagg atggcgaaaa agaaaaacta gttctttgga
    11161 tgccaccagt agaaaataat atgaaggaag tttgggaccc tattttagat aaatttgagg
    11221 aaaaaaataa ctgtgaagta gatttacaga taattccttg ggataattat tctgaaaaat
    11281 ttgcaacagc tataagtgct ggcgaaggtc cagatgtagg atatatgtat gcagaaatgt
    11341 atccacagtt tatagaaagt ggagcagtag aagatttgac taactatgca actaaagagg
    11401 ataaagagca atctatttat ataaaaacat ctgaaatgat gggtggaatg tatggtatgc
    11461 cattccaagc agcaaatcca ggggtattat actataacaa ggatatttta gagaaactag
    11521 gagaaaaacc tcctaaaaca tgggaagatt ttaaaagaat ttgtaaaaaa gcaacgcaag
    11581 atactgatgg tgatggaaag atagaccaat ggggattagc tcagggatgg ggagctaaaa
    11641 cttttggtaa cttaaactgg aactggtatc cttatttatg gcaagctggt ggagatatat
    11701 ttaatgatga tttaaaatcg gttaaattta atgataagag tggtttagaa gcagctaagt
    11761 tcttaaaaga attacaagca tatgtgcctg aagattcttt atctaaagat agtaatgaaa
    11821 tgatagaaag tgtatttgga ccagggaaag cagcatttac aataatgtta tcttctgctg
    11881 caacaagtgt atttgatgaa tcattcccgg atttaaattg gggatttgta actggactag
    11941 aagataagaa agcagcaact tttggagcag tagaccattt atctttaatc tcatctgcga
    12001 aaaataaaga acttgcatac aagttaatac aacatatgtt aagtgttgaa tcaatgacag
    12061 aatttcataa agcaatacca agagcaccaa taacaaaagg tgaaccatat caaggtgatg
    12121 aaagattcaa agaaatggtg gaaaatgaca aaaatgtata tagaccatta gtagtaggac
    12181 ctcatggagt tgaaatatat gaatatttat ggaaagaact tcaaactatg atttcaggag
    12241 ataaaactcc aaaacaagcg ttagatgatg ctgcaaaata ttcaaatgat ttattagcac
    12301 aataaaagtt taaagctgta atatgttgta aagtacttta cagattaaaa ttagcattgt
    12361 atttataaat acaatgctaa tatttatatt ggagaggata aaatggaaaa cttagttaga
    12421 gagtttgaag aaaattcaaa aagaaaaaaa acaaaattaa atattaaacc atatatgtat
    12481 atattgccat tgggaataat tttagtggcg ttttatgtga taccaataat aatgtcaata
    12541 tactttagtt ttacaaagta taatattatt agtccagcta cttttatagg tttagaaaat
    12601 tataaaaaat tatttacaga tgaaacatta aaagtatcta ttataaatac tattaaattt
    12661 actgttgttg tagtaccttg ccaaactata ttatcattga ttttggctgt ttggattaca
    12721 ggaaaaggaa atagtaaaat agcttcattt gctaaaggtg ctatatttat accagtatta
    12781 tcatctatgg ttttgatagg tatggtatgg agggctttat tgaatggaga aggttctatt
    12841 atatatcagg tattaggtgc ctttggaata gaatcatcta aacttttagg agatagtaag
    12901 acagcattac ctacacttat gtttatatct atgtggaaaa atatagggta tttcatggta
    12961 atttatattt cagctataat gaatttacca aaacactgct atgaagtagc gaaggttgat
    13021 ggggctacaa aacttcaaga atttataaaa ataactgtac ctttattgaa accaactaca
    13081 ataatggttg tatttttagg gtcgatatgg tcattacaag tatttgactt aatttatact
    13141 gtgacaggtg gaggtccagg tatatctact atgagtatag ttatgcatgc atttaactta
    13201 aactttaaaa actttaattc tggatatgca atgactgtag caaacgtatt atttttgtta
    13261 atagcagtag tttcgatttt acaaaataag cttgtaaaac gagacaattc agatttttag
    13321 aaaggtaggt ataatatgaa aacagcaaga agattattaa gtgatggttt acttttattt
    13381 atagcgtgtg caagtttggt accttttata tacatgttga taatatcatt gaagataaca
    13441 tataactcat atagtttgga tatatctttt tcaacagtaa cccttcaaaa ctatatagat
    13501 atttttacta aaaaaggatt tgcacaatat ttttttaata cagcaatagt atctttttca
    13561 ggtgtattat taaatttagt atttagtaca ttagcaggat attcttttgc gaagatggat
    13621 tttaaaggta gtgataagtt atttttattt atgataatga ctttaattat accttcccaa
    13681 gttacaatga taccactata tataataatg aaacacttag gatggataaa tagttatctt
    13741 gcacttatta tgccaatacc aactgcattt ggcgtattta taatgagaca agctatttta
    13801 ggagtaccaa aagagttatt agaatcagca aagatagatg gttgttcgga ttttagaatt
    13861 ttaatacaaa tagtgttgcc tttaattaaa ccagcattaa ttacactagc aatatttact
    13921 tttatgggag catggaacga gtttatgtgg cctcttatag ctacaactaa agatgcaatg
    13981 agaacattaa cagttggatt atctactctt aagacattcc agataactaa ttatggacag
    14041 atgatggcag gggctacaat aacattttta cctccattta ttttctattt gattttacag
    14101 agtaaatttg ttgaaggtgt gtctttgtct ggtataaaag gttaaatagt ctaatttaat
    14161 aatatataaa gaaacttata taaatatgga ggaatttaaa ctatggaaat ttcatatgta
    14221 ctgaaagatt gcacattcaa acacaataaa tactcagttg tagaagctga aacttggaaa
    14281 gataaggatt tggatattgt agtagctaaa ggtgaaaaat ttgcatttca aattatgtta
    14341 aaagcaacag aagaatttaa ttgtacaatt gataaaagta gtgctatatc atggaaaggc
    14401 ctgggaaata gagtaaggtt agcattaaat atgcctaata gtttagaaaa taattttagt
    14461 ataaatatat taggatatgt tcaagatgat acaaaggcat ttgtgaatga tgctatttta
    14521 agggataaag atgtgttagt agagcaatat ttgcctcaaa cattttggat tgaaggtcaa
    14581 gtaccagaag attttgaaga aaataacctt gatataggta ttgatatatt taaaagcttt
    14641 ggatatgaag atgaagaaaa agtttgtaca attgatgttc ctgtaaaagt aagaaatgtt
    14701 gtgctaaagc ctttagatga aagcaagttc tttttagatt tatggcaaca tccaagctgt
    14761 ttagctagaa tgtataaagt tgagttatgg tctgatatac attttgaaat agtcgataac
    14821 tatttaaaag aattagcatc tttaggagaa aaagttgcaa cagttatagt ttcagattat
    14881 ccatgggcag ggcaaagttg ttataaagtt tataaaaatc catcaaactt atatgaatat
    14941 aacatggtaa gtgtatcaaa aggattggat ggaaagatta aatgtaactt tgagagtatg
    15001 gataggtata taggcatagc tgataagtat aaaatggcta aggagataga tttatttggt
    15061 ctattaggta attggtgtgc tggtgagttt ggaaatcctg tagaaggtta taaggaccct
    15121 ataagagtaa gatattttga tgaaaaggat aaagtattta aatttataaa caatacaaat
    15181 gatttaaaag aatatatagg attagtatta aatcatttaa tagagtgtgg attatgggac
    15241 agagtaagga ttatagctga tgaaccaaat aatccagaag tagtcaaaga gtgtatagaa
    15301 tttataaatt ctactgtagg tactcatcaa gttaaatata aatcagcaac tcacgaccaa
    15361 aactttttag acagagctaa agatgaaatt gatgatatgt caataaattt aaaacttact
    15421 atacaaaatt ataaagatat agaaagtctt aagaaaaaaa tcaatgacaa gggtgggata
    15481 ttgacttggt ttgtatgctg ttttccagaa aaaccaaata gttttttaag ttctcctttt
    15541 gtagaaaata gaataatagg atggtatact tattactttg gtcttgatgg atttttaaga
    15601 tgggactata atttatggac agaagaccct tggaaagatt caagttacaa attccctatt
    15661 tggaaagctg gagatatgtt ctttgtttat cctggaaaag atttaaagcc agttagaagt
    15721 gtcagaatgg agaatttaag atttggaata caggattttg agttatttac aatgttagaa
    15781 aaagaaaagg taagagagta tatagaagtt gagcttatgc aagaattatt aaataaaaaa
    15841 gaaaatgctg aaattaaagg ttttggtgac atagaactag gatattcact tgataattgg
    15901 agatatgata aagttaaaaa atatgtatta gagttgttag acagagtata aaaaatataa
    15961 gagtgtctca aaatgaactt tttagttcat gaggcactct ttttgtatga aatcaatata
    16021 gtttcattgt ttcaacaatg aaaaatagga ttatttctat tctaagacag ccctttagtt
    16081 ttttagttgt attcaatgtt ttaaaactaa ctataaacgc tataaagttt ataaatgaca
    16141 ataattatat ataacatgat gtactttaat ttgctataat agggattgaa taatattata
    16201 tggaagataa cattgtttgg ggggattaat ataaaaagaa taggtattat tataatgttt
    16261 tttttaagta tatcttttat tggattttat cttgtaataa gagataacag tgatacagtt
    16321 aagatgcaac gagggataaa tataggtaat gcactagaat cacctaagga ttttccgtgg
    16381 gatgtaaaaa tgtcaaataa gttttttgat gatataaaag atgcaggttt tgacacagta
    16441 cgtataccag taaggttttc tgattatacg tcagattctg ataattttaa aattgatgaa
    16501 gaatttttta aaaagattga taaatatgta gattatgcat tggataaaga cttaatagtt
    16561 gtgttggatt tacaccattt tgaagaaatt atgaaagaac caagagttca taaagaaaag
    16621 tttttaaaaa tatggcaaca gatagctaat aggtatcaaa aatatgataa aaaattggta
    16681 tttgaattat taaatgaacc taaagaaaat ttatcttcac aattactaaa cgaatacatt
    16741 gaagaagcaa taaagataat tagaaaaact aaccccaaaa gaactattat agtaggacca
    16801 tataatttct atcaaattga ttatttgaat gaattaaata ttcctaaaga ttctaatata
    16861 gtagtttctt ttcactatta cgagccaaat gattttgcat ttcaaggaaa tatctatcat
    16921 aagggatttg agcacttaag caatattact tgggaaggaa caaatgaaca aatggattat
    16981 ttaaaaaaaa ggtttgatac tgtggaaaat tgggcaaata aaaataaggt aaaaatcttc
    17041 ttgggtgaat ttggaataac aaaagaggca ccagaagttt caagaagagc atggataaaa
    17101 gctgtaagag aagaagctga aaaacgaaac ttttcatggg cttattggga gcttgcatct
    17161 ggatttggta tatacaatca aattgaaggg acttgggata gagatatatt aagtgcatta
    17221 atagaaaaga ggtgatttta tgaagaaaaa ttcaaaaaag atacttataa ctttaactat
    17281 tattactaat gtcatatata tattgtggag aattttttac acagtaccta aagaagaagg
    17341 gaagtttgcc ttaatatgtg ccataatact tctttttgta gagataatgg gtatgatgga
    17401 aatgtttgtg cattattatg gtatgtcaaa tattgagtat cctgaaaaac caatcataag
    17461 tgaagaactt tatccacatg tagatgtatt tattgcaaca tataatgaat ctgttgattt
    17521 ggttagaaaa actgtaaatg gatgtattca tatgcagtat ccagataaga aaaaggtaca
    17581 tatatacata tgtgatgatg gaaatcgtga agaaatgcgt attctagcag aaaaaatggg
    17641 tgtaaattac ataactagaa ctgaaagaga aggagcaaaa gctggaaatt taaataatgc
    17701 aatgcaacat acaacttctc ctttgattgc tacatttgat gcagatatga taccaatgca
    17761 tgacttttta attgctacag taccatactt tttaaaaaat gagcaagcta aaaaagatgg
    17821 agaaaaagag gagtatgaaa aggttggttt tgtgcaaact ccacagagtt tttataatcc
    17881 agatttgttc caatttaatc tgcactctga aggaaggatt ccaaatgagc aagattattt
    17941 ttacagagat attcaattgg ctagaaatag gacaaattct gtaatttatg gtggtagtaa
    18001 tacagttatt tcaagggaag cacttgaaga agttgatgga ttttatactt attctattac
    18061 agaagatttt gcaacaggta tcttaattca aagtaagggg tatagatgtt atgctattcc
    18121 agaagtacat gcatcaggtt tatctcctac agatttaaaa agtcttatta agcaacgtga
    18181 aagatgggca cgtggatgta tacaaactgg tagacgtcta aatattttgt ttaggagagg
    18241 tttaggtttt tggcagaaaa taagttatat ttcttcaatt acttattggt atgcaagtat
    18301 aaaacgcttt gtatatataa tggcacctat tttattttct gtgtttaatg tgattgtagt
    18361 aaaatgtacg ttacttcaag tattggtatt ttggttgcca atgtatattt taagtagttt
    18421 atctcttaaa atattttctc aaaatataag aaatactaga tggaccaata tatatgaaac
    18481 tataatgttt cagtctttaa tgccagcagt aatacttgaa acgtttgcaa tatctaaaaa
    18541 taaattttca gtaacaaata agagtaaatt agaagaaaat agaatgtata agtttttaca
    18601 gggaatacct tattttattt acatggtctt atctatcata ggaattttaa aaatgtttgt
    18661 agcaatattt aaaatgagtt caatgacata ttcagttgta ttattttggt tgattggaaa
    18721 cctttttaac cttgttatgg ctacattatt tatatcaggg agacagcagt taagaaagtc
    18781 tgagcgttat attgctgaaa ttgattttaa gttaaagcaa aattcttatg ttctttcttc
    18841 aaaaacaatt gatatatcag aaaatgggtt tgcatttttg ctagaaaatc cagaatatat
    18901 ttcaccagaa gaagagtttg aggtagagtt tagagaaaaa tctggaaatg aaatgtatat
    18961 agccaatatg aaagcaaaga ttgttaatgt tgtggaagta aattcaaagt ggaaatatgc
    19021 agcttatatt acacatattg aagatagtga aatagataat tggatgtgta ttgttcacga
    19081 tagaatacct actttaccaa tgacaatatc taatcaacta ggtttctttg atgatttgca
    19141 aattaatgtg aaaaaacgta ttgaaaaaac tagaacatta tcacgaagaa gtccaagaat
    19201 caatatgaat tttcagatgg atataaaaaa tacaggtaaa ttaagaatag tgaattttaa
    19261 ttatcaatat gtattattaa attttgagaa taaaagtatt tatcctaaag aaatagcatt
    19321 ggaaattaat gaaaatattg tgttagaatg tgatttgtgt gagggtaaaa ttgatgaaag
    19381 aggtatctta tataaagtaa ataacattga cagcataatg caaaatttct ttttaagaga
    19441 tgaaatgatg gattggatat tacaaaataa gacaattctt gtttcaaagc ctagtgaaaa
    19501 gaaggaaaaa agtatagatg agtttgaacc catggaatat atttaggaga gatgaaatga
    19561 agactttaaa agtgtttttt tgttctacaa aatatcttat tttggtgttt atttttttta
    19621 taaatactac ttttataagt tcagcaacaa gtgataaaat tttagatttg tcctttaaaa
    19681 agatagagac tgacttaaat tctaagatta catatgaaga tacaggtgta aaaatagaaa
    19741 cggatagttc taaaagcgat aaggaacgtt atttatatat ttaccaaaat ataaaagaaa
    19801 attggagcat gtataataat ttttatattg aaattcaaaa taaaaataaa agttctcaga
    19861 gaataaattt aagcatacaa agtaaaaata tgcttgaatt tagattaaaa gaaggtagtg
    19921 aagtattttt agaaggtaaa aatataatat attcagataa aataaaagaa ggatgcatag
    19981 aagtaccagg tgaatttgaa ggcaaaattt atgtgaactt taatagcctt ataaatgagg
    20041 aaagtaatgt tgtgctggac tcaaatatgc tttcaaacat tgttagttgg ggaattacgt
    20101 ttattccatc tgatgaagaa cataatattg ttattataaa aaagatatct ttattgtctg
    20161 aagagaaatt aagattttta aataatatta aaataattgg cgatgaagaa gtacaaatac
    20221 cagtattggg tcaaagtata agtcagtatg aagtattagg tcttaaaagt gattctaaaa
    20281 ttaagtactc tcttatggga aaacaagata atgtaagcat atctcaaaaa ggtaaattaa
    20341 cattaagtaa taaagccaaa cctggacaaa ttattttaca agttaatgtt gatgataagt
    20401 ttaaaattgg aaagaaaata acattaactg agtcttggag tgttaataaa aaagataagg
    20461 atggtgttcc atatacatta gtatatcctg agcaatcacc aacagtacaa gatatgaaaa
    20521 aaataaactt tatgaataac atcataacgt ttgttcgaat tttatttgta tctctagtaa
    20581 taatatgctt tggaatatat ttatattgga aaaaatgttc aaaaactaag taaagagagg
    20641 aggttagatt gtgttatttt caagtttaat ttttttattt tattttttac ctataacttt
    20701 ggttttatat tatgtattta ggtttaaccg aacgatacag aacatgatat tgcttgctgt
    20761 tagtttattt ttttatgctt ggggagagcc aaaatttgtt gttatcatga tagtttccat
    20821 aataatgaac tatatatttg gtttactggt agatagatat agggaaagta aaataaaagt
    20881 aaaagttttt ttagttttga tgtgtgcata caatataggt gttttattta tatttaaata
    20941 tctagctttt gcgctaagaa atattagtga acttattagt actgaattga ctatacctag
    21001 tattgtttta cccataggaa tttcattttt tacatttcag ggaatgtctt atgttataga
    21061 tgtatatcgt agacatggag aagtccaaaa gaaccctttt tatgtgggat tgtatatagc
    21121 atttttccca caattaatag caggtcctat agttagatat gagagtgtgg cagaacaaat
    21181 tctcaataga aaagaaactt ggaataaatt ttctattggg acttgtaggt ttataacagg
    21241 tctaggtaaa aaagtattaa tttcaaataa catggctata gtagcagact atatttacac
    21301 tatgaatagt caaggagaaa tagctgcatc acttgcatgg cttggttcaa tagcctatac
    21361 tctgcaaata ttctttgatt tttctgctta ttcagatatg gctataggct taggtcttat
    21421 gtttggtttt aaatttgaag agaactttaa ttatccatat atatcaaaat caattagtga
    21481 gttttggaga agatggcaca tttctttggg aatgtggttt aagagctata tatactttcc
    21541 gcttggaggt tctagagtag caaataaaga tattatgatt agaaatatgt ttatagtgtg
    21601 gttatttact ggaatatggc atggagcaga atggacattt gtaatttggg ggattttaaa
    21661 ctttgttttc ctaattatag agcgatttat aatgtttgaa aagattgaaa atcacaattt
    21721 tataaaacat atttatgctt tattagtagt taattttggg tgggtattat ttaggtcacc
    21781 taatttaaaa gaagcatata attattttaa agctatgttt ggaggcaatg gagctatatg
    21841 gagtgattat acatatatgt ttttaaaaga atactgggtt ttctttatat ttgcatttat
    21901 ttttagtgta cctattgcaa agaaaatcaa taagtttgtt gttgaacaag ctaaatatag
    21961 tatgatattt aacgtatttt atccaatctc agctatttta ttgtttttta tttctgtgac
    22021 atatttagta actggaagct ataatccatt tatttatttt aatttttagt tgggaggaaa
    22081 agtatgaata gattttttaa agtaagatta ataactgcaa ttatgttctt aattatttta
    22141 ttcgctttct ctatctttaa tgtttgttat tctggaaaag atacgataca ggaaataaaa
    22201 aattcaatag ctgaaaaaag tagtttaaaa gatacaatat catctattga taatacagtg
    22261 aatgacaaag taatatacaa aaatacgttc attgaaactt atggctattt acaaaaatta
    22321 atgggtaaaa atgagttttc aaaatttagt gttgtaaaag atacttctgg aaagatgcat
    22381 tatacatatt ttgctaaagc tccaaatcca gttggtgtga tagcagatag ggtaagaaaa
    22441 ttttctgatg aaatggaaaa gcaaaacact aaattggttt acctaatgac tccagataag
    22501 tatataaaag gagtgacaaa atttccaaaa ggaatacctt attcttatag taatgagaca
    22561 gcagataatt ttttaaatca actaaaaaag tataatgtgg actatattga ttttagagaa
    22621 aatattttaa agagtggtat tccaaaagat gatttatttt ttaaaacaga ccatcactgg
    22681 aaagtagaaa cttctttttg ggcatttgga gaattagttg agcaacttaa taaaaaatac
    22741 aatatgaatt tagatgaaaa tcattattat agagataagg acaattataa ttcaattgta
    22801 tatcctaagt catttcttgg ttctatggga agaaagactg gtatcttata tggtggtata
    22861 gatgatttta cattaattta tcctaaattt aagactaatt atacttatta tacagattct
    22921 aaatctcaaa aatttgaatt aaaaggaagg tttgaagaga gtttaatatt atcatatcct
    22981 tttaatgcag accttgattt aatggatggt caatcagata agtattttac atatttattg
    23041 gggaacagac cattagttaa aataaaaaat atagaaaatc cagatggatt aaaagtgctg
    23101 tttgttaaag attctctaat tgtaccaaca gcagcatttt tttcctctgt ttgttcatct
    23161 atagatatga tagaccctag atattatgat ggagatatac tagaatatgc aaaatcccat
    23221 gactatgact ttgtttttat atctgtatat ccccaaaatc ttacaaaaga attttttccg
    23281 ttttgtgagt aaatttctaa aaaagttttc tgataaaata aatggctaac tggtaaaaag
    23341 ttagccattt attttgagtg ttattctaag aaatattttt tttactaaat acttctttat
    23401 ctatttttcc aagtgatttt cctacagtaa ttgatgatac catagcacca ttaacattta
    23461 tcatagtacg tcccatatct agtatagggt ctattgcaag tataccacca actaatggaa
    23521 aataagaacc aagaccaact ccagagataa caactgaaac ggccattgta gcagttcctg
    23581 gaagaccagc tattccaaac gaactaattg ttataacaat taacaacata acataaaagc
    23641 tcatatccat gtttactcca gccatattgg caagtgttac agccattaaa gcaggatata
    23701 tacctgcaca accattcata cccatatttg cacctaaact tgctgtaaag cttgatacac
    23761 cctcatcaac tccgtgattt tgtactagac tttctatcgt tacaggcaat gttcctaaac
    23821 tagaacgtga agtaaatgcc agaagtaaag cttcagttga attttttata taagtaatgg
    23881 gatttagacc atttaaagtt ataaaaatta aatgtataat aaataatatt gcaactgata
    23941 tataaagagc taatataaac tgtaaaacag atactattga actaatacct tgtgaagtta
    24001 ttgtatttgc aagcagagca accacagcat aaggcataaa tttaataaca gtcatagaca
    24061 cacttacaat tattttgtaa gatgcttcta taaaacctat aaatggtttt attgtttcaa
    24121 aatgtttttt acttaatctc tttattgagg tagctataaa tgtagcaaat attattgtag
    24181 ctacaatatt tccatttgcc atagccatta caggattaga tggtagtaat cctcttaaag
    24241 tatccacaag agagtttatt tctttaagtt cttgattttc aactaaagta ggcatattag
    24301 ctcctacacc taacttcatt atatttgcaa cgataattcc aacaattgca gctatagcag
    24361 tagtagcaag aagcatacct aaagatttaa atgtcagagt tccaagattg ttatctttca
    24421 tatttataat tacttttata atggataaga aaactaaagg tactactaac atttttaata
    24481 agtccataaa tccagaacca aataggctat accacttact tacttcttgt agccaaatga
    24541 cttgagttgt atcattagga aaaccagcaa aagcttgtat tatgattccc aatatgagtc
    24601 ctagtactgt agcatatatt gttctcaatg aaaattttat ttttttcttt tcaaattgtc
    24661 taacaataaa gaacgtacct agaagtaata ttatgaaaaa aattgtttta aaatctgtaa
    24721 tcataagaaa ttgtgataaa aatgaacttt ccaatatttt tctcctttta tttaaaaata
    24781 aatttttcta agtttaaatg atatcaatat attaaaaaaa tgtcaatatt atatcacaat
    24841 ttacttaaat agagtaaaac aagttgattt tgaaaaatgt gattaagtac agaaaaatag
    24901 ggtataataa atgtttaaag aatttgaatt gtggtaagaa aataaaaaat agcttttata
    24961 agatttgatt tcaaattaaa atgtgttaaa taaataacta aaaattatta taaaaaatgt
    25021 tttcatagaa tatatatcaa atgaaaagct taaaataagt gaagaaatta actagacatc
    25081 aaaggagaga taaaatgaga gtaataataa ttggtggtgt tgcagctggt atgagtgctg
    25141 cagcaaaatt aaaaagaata aaacctgagt atgaagttgt tgtttatgaa aaaactgaaa
    25201 tagtatcttt tggtgcttgt ggattaccat actttgttgg tggatttttt aatgatgctg
    25261 atgaattatt agcaagaaca ccagagaaac ttagagaagc tggaattgat ttaaatatat
    25321 ttagagaagt cgttgaggtt gatagtgaat ctaagaaaat aaaagtaaaa aacataaaaa
    25381 cagatgaaat atatgaagat tactatgata aattgatgat agcaactgga gcaagaagta
    25441 ttatgcctcc tataaaaaac ataaaattaa agaatgtttc tacattaaaa agtttatatg
    25501 atggagaata tttaaaaaaa cttttaagta atgaagataa taaaagagtg acaataatag
    25561 gagctggttt tatagggtta gaagcagttg aggcatgtaa gaaattaggt aaagatgtag
    25621 atgtaattca gttagaagat agaatattgc cacaagtatt tgataaggaa atgacggatg
    25681 tattagaaga agaaataaat agacataatg ttaatttgca cttagatgaa atggttgtag
    25741 aactttgtgg agaagataaa gtagaaaaag ttataacaaa caaaggggaa atagatacag
    25801 atgtagtaat aattgctact ggcgtgagac ctaataccga gtttttgtca aatactaata
    25861 taaaaatgct aaaaaatgga gctatagtag ttgatgagta tggaagaact tctgtagaag
    25921 atatatactc tgctggagat tgtgcaacta taaagaatat agtaagtaat gaaaatgtat
    25981 atgttccttt agctactgga gctaataaat tgggtaggat agtaggagaa aacctagcag
    26041 gaagagaaat atcttatcaa ggttcactat cttcaagctg tataaaaatt atggatatgg
    26101 aggcagcatc aactggaatt acagaaaggc aagctaaaga tttaggcata aatgtcaaat
    26161 ctaaatttat ttctgattat aatcaaactc attattatcc aggaagaaat aaaatatatg
    26221 taaagcttat ttatgatgca gatacaaaag ttattttagg cggacaagtg gctgggttta
    26281 aggatgctgt ccaaagagca aatgtattgg cagctgctat ctttggaaaa atgaccacaa
    26341 atcaacttgg aatgttagat ttatgttatg ctccaccatt tgcaagaaca tgggatgttt
    26401 taaatgtagc tggaaatgta tctaaataat atttagatta tagctaatat agttgacaag
    26461 caattaaggg aatgaattta cctaaactat ttttatcatg gtaaaattca ttcctaaatt
    26521 tttattccaa atttttattc caaattttta gaaataagga ttccattaaa ttattcatca
    26581 aaatttaaac gcaagcttaa tctatcttct ccaagaatag tattttcaaa aacattagta
    26641 gcattttcta aatacatact aggtatgtct aaagaaggac taaaatgagt cagtaaaagt
    26701 tgtttgacat ttccaagttt tgcaagatta gcagcttctc taaaagtcat atgtttgttt
    26761 ctaacagctt ttgatatatc taaatcatca ccatacatgg cttcacatat aaataaatca
    26821 ctatctttta tgaaatctgg tattgattct ataggtcttg tatcggttgt aagacttatc
    26881 ttaactccct ttctcaattc tcctaagacc atatctggag tgtattgttt tgaatctaaa
    26941 actatatttt gaccttcttg gagtttgttc cataatattt taggaacttc gtttttagtt
    27001 gccttatcta tatcaaattt agggtttctt ctaaaattaa aactgtatcc taaacactct
    27061 atagagtgtt ctaaactgat tgttgaaatt tctaaatttc ttaaaacttt attatctaaa
    27121 gaaaaattgc cttgagggtt ttctataatt tttaaagtgt atggtacata ttctactaaa
    27181 tttctcatag aacgtataca atcgactatt ccaactggac caactatagt taaatcactt
    27241 gtacgaccac tattcccaat tgttgataaa agtcctaaaa gtccaaatat atggtcacca
    27301 tgtaaatggg ttatacaaat caagtctata tctttaaagc cacatttttt tagcttcata
    27361 gatacctgag taccttcacc acagtcaatt agtatttttc tacctttata gtttataaat
    27421 acagaggata gaaatctgtt tggcataggt acatttccac cacatccaag taaagttaaa
    27481 tctatcattt tttccccttt ctaaaattaa cacaaatctg ttgtttgttt ttattatgat
    27541 atcctattat aattatttta acattaaata taacaataaa tgtgtatttg agtcaagaaa
    27601 tattgtcaaa taaagttttt atatagcaat gtataaaaat ttggaaaacg aaaagtgaaa
    27661 attatattta ggagtattta aaggaggaaa atttaatgaa agtattaaag aaaattacta
    27721 ttttgtcatt aattacatta accttagttg gttgtagtaa cggagaagag aaaaaagtgg
    27781 ttgaaggttc taagaaaact caaaattcta aaaaagatga acagcctata atagtacata
    27841 aagatgcaaa tatattgaaa gataggtatg agccaccaaa aggatttaaa agagttgatg
    27901 tagaaaaagg tagttttgaa tcatttctta ggaatactaa gctaaaaaat tatggagaga
    27961 aagtaaagta ttatgatgga agaactaagg aatcaaaagg tatttatgat agtgtatttg
    28021 atgttgatat tggaaataga gatttacatc aatgtgcaga tgcaattatg cttatgagag
    28081 cagaatactt gtatcagaat aaactatatg atgaaataag ttttgatttt gttgatggtt
    28141 ttaaggcgaa gtattcaaaa tgggcacaag gatatagaat ttcagtaaaa gatagtggtt
    28201 caagctggta taaagcaact gaatattcaa catcttatga gagtttcaga aagtttatgg
    28261 atatagtgtt tgcatattca ggaacgttat ctcttgaaaa agaattggag cctgtaaaag
    28321 ttgaggatat gaaaatagga gatgtgttta tagttggtgg aagtccagga catgcaagta
    28381 taattatgga tatggcagaa aatgaaaaga ctggtgaaaa ggtatttatg atagcccaaa
    28441 gctatatgcc tgcacaacaa acgcaacttc ttattaatcg taaaaatcca aatttgagtc
    28501 cttggtatag tttagatttt gaaggtgatt tgacaatacc tcaatggact tttaaaagag
    28561 accagttgaa aagattttaa aataataatt ttatataata aaagaaaaat atagatgtga
    28621 aaaaatgtaa ttagattaaa actaattgga tatggacaaa attaaattga tagataaata
    28681 ttaaaaatta cactaattaa tcttactata gtttaatatt tatctaaaaa gttttatgta
    28741 aaatataaat tttatattaa agctaaaact aatttgagga ggctaattta tattgaagat
    28801 tagaaggtat ttaattttgg taattataat tctaattaca acaagtcaga gttttatttc
    28861 ttatgggcta gagcgatttg aagaaataac catttttcac actaatgata tacatggaag
    28921 atatgctgaa ggtgatgacc atatacaaat aggaaatcta gccacattga aaaaagaaac
    28981 tccaaactca attttagtag atgcaggaga ttgtttgcat ggacttccaa tagtaaatat
    29041 ggatagaggg aaaagtgcaa ttgaattaat taaatgtgct ggatatgact atattactcc
    29101 tggaaatcat gactttaatt atggaaaaga tagactgttt gagttatcta aaagtgctaa
    29161 tttaggagag aatactttaa aatttttatc atcaaatatt tttgaaaatg ggaaaaaagt
    29221 ttttgatagt aatgatataa aagaaataga tggaataaaa ataggatttt ttggactctc
    29281 aacccaggaa acaaagagca aaactggatt taaaaatgtg gaaggattag agtttaaaaa
    29341 tcctatagaa agtgcaacag aacaagtaaa tgaattaaaa ggaaagggtg ccaatgtaat
    29401 agttgcaata tgtcatattg gtacaaatag tttaagtaag ccaaatagta tagatatagc
    29461 aaatgaggtt caaggaattg atttgattat tgatggacac agccacacta agtttgaaaa
    29521 tggtaaacaa gtgggtgata cattgattgt aagtgcagga caatatttag aaaatattgg
    29581 cgagataaaa ctaactttgg atactaatga tgtagaagat ataaaaatag aaaataaatc
    29641 agctagatta attggaaaag aagaagcatt aaagtataag cttgactccg ttgtaagtaa
    29701 aaaggttgat aaaataaaag aacaacaaga aaaaatatta gataaagtta taggggtaac
    29761 tacaaatact ctagatggtt catatgaaaa tgtaaggaca aaagaaacta atttgggtaa
    29821 tttaataagt gacattttac tagataaaac taaggctgat atttctttat ttaatggagg
    29881 gaatattcga gatacgatag aaaaaggcaa tataacaaga agagatattg tagatgtatt
    29941 tccttttagc aatactatag ttaccaaaga acttacagga gcacagatta aagatgtatt
    30001 ggagcatgga gtaaaacttt atccagaaaa aagttcagcc tttttgcaag tgggaggaat
    30061 ttcatattac tttaatccta aacaaaaaga aggcgaaaaa ataacaaata ttcaaaaaga
    30121 aggaaagcca ttagatttaa ataaaaagta tgtagttgca acgaatgatt atatagcatc
    30181 aggtggagat gaatttcctt gtttttcaaa agagccagta ctaaaggaat ttggaaattt
    30241 agagagtgca gtcattgaat atatagagta caaaaaagaa atatcaaaaa atgtagatgg
    30301 aagaattggt attaaatcta aagatgagtt aataagcaag aatcatgaga gtgatttgat
    30361 attaagtgaa aatgaaaaaa atatcaatga taatgaagag aatctatcta ataatataaa
    30421 aagctctgat agtgataaaa ataaaagctt agataaaaca agttctagag aaagttctaa
    30481 tctaggaata tctaaaagag aagctaaaga aaaatctcct aaaacaggag atttaggttt
    30541 ttcaaatagt ataattatat ttatagtttc aagtacattg atatgtttat tgaattttaa
    30601 tcaaaaagag ttaaaggata aaaagtcaaa ataaaaatta atgagcaaaa attagtagat
    30661 agtaatttaa ttttagataa tagtaaacag caaaggaaat tagttatata aatactaatt
    30721 tcctttgttt tatgttttaa catgattact tttaaataaa attgtatttt atgaaaaata
    30781 attatactaa atggcatagt attatgggga tttttagttt ttattcaagt aaatgaaatc
    30841 atattaacaa cttctgatat atttacaaat tgataaaaaa atgattttat tagtatgata
    30901 taatttagct agtatgtatg ataatggagg atatagggat gaataatata ttacttttgg
    30961 aagatgacaa atctttaaat agaggaatta gttttaaatt aaaaaaagaa ggatacaatg
    31021 tttttagtgc atttggtata gaagaagcaa aaaatatttt cgcaaaagaa gagatatgct
    31081 taattatttc cgatataggt ctacctgatg gtagtggttt tgacttttgt gaggagatta
    31141 gaaagaaaag tgatgtttac ataataatgt tgacagctct tgatgaagag gtggatattg
    31201 tcacaggata tgatttaggt gcagatgatt atatcacaaa gccttttagc ttgatggtat
    31261 taatatcaaa agtgaatgcg cttatgaaaa gagtaaatac agtaaaaaat tatactctat
    31321 tagtttgtga tgatttattc ttttattata tagaaaataa attaattata agaacagatg
    31381 ataaggaaga agagataatt ttaagtaaga cagagacaaa actattaaaa tatttgatgg
    31441 aaaattctat gcagacattg actaaagagc aactgttaga atctttatgg gatagtagtg
    31501 gtaattttgt agatgataat acgattgcag taaatataag aagacttaga caaaaggttg
    31561 aaaaaaatcc atctgaacct aaatatataa aaacagtaag aggggttgga tatatgtggg
    31621 gagaaaggag tataaaaaaa tgctaaaaaa tatgaacctc aaagatggtt taagtgtgaa
    31681 agatgattat ataatgagaa gagttattgt atattctgtt ttatctgtac taatagtaac
    31741 ccttgtattt atactttttg aatatagcag attacataaa ttgtatgaac atcaaataaa
    31801 aaatactcaa aatgttgctg gaatattgat tgaaaaatat cctaatgatg aaatagatat
    31861 agtaaattct atttataatt ctgattatag taaggtgtct actggagaaa aggcatttaa
    31921 gaaatttggc tatggattgg aaaataaaat gtcaaatgat aaaaatttta gcttatactt
    31981 aaaatctttt tttaaggaat cattttttat ttttttaatg atgtttatag tagttatagg
    32041 tataatatat tattttataa gatacataaa taaaagattg agtaaaattt attttatagt
    32101 agataagatg tcacaagaaa actacttgaa ggacactact gagtcagaag aaactggtta
    32161 tctagtagaa tataatgaat actttaaaga aggcatattt agtaagataa ataactgtct
    32221 atatgagctt aatagaagtt taaagataaa gtttattaaa ttagaaaaag aaaaagaaag
    32281 tgtaaaatca ttagtaacag atatttccca tcaattaaaa actcctttag catccttaaa
    32341 attatacaat acattactta tagaagaaga attagaagaa gatgaagcca acgagttctt
    32401 attaaccaat aaggcttcta taaataaatt agaaaattta ataaattctt tagtgaacat
    32461 atcaaggtta gaaattagca tgataaacat aaaaaaagaa gataatgata taaaaaatac
    32521 tattctaaat gcagtaaaaa gtgttacccc taaggctaag agcaaaagta taacattaaa
    32581 tttaaatgaa tttaacagta gaattatacc acatgataaa aagtggactg aggagagtat
    32641 ttttaatgta ttagacaatg ctgtaaagta tacacaggta aatggagaaa taaatatatc
    32701 tgtagaagaa actgctaatt tctttaaaat tataatagag gataatggca tagggattga
    32761 aaaaagtgag tttaataata tatttaaacg tttttataga ggttctgctg atgaaattga
    32821 atctgtagaa ggtagtgggg taggattgta tttaagtaga aagattttag aagaacaagg
    32881 tggaaatatt atagtttctt caaaagtagg aatagggagc aagttttctt tgtttcttac
    32941 aactatgtaa tattaaatga aaggaaaatg taagcctcta catttattat ataagtataa
    33001 acagctccag aaataaaaaa ttaataaaat atggagtaat ataacttaga atgagaggat
    33061 aattttatga aaatactata tactgaaaat ttatcaaagc attatggaaa aggagaaagc
    33121 ttagtaagag ctttggacaa tgttgactta gaaataaatg aaggtgaatt tgttgcaata
    33181 ataggaaaat caggttctgg aaaaagtaca cttttgcata tgattggagg tcttgatata
    33241 cctacatctg ggaaggtgta tatagataat aaaaatatat ttacattaaa agaagaagaa
    33301 cttgctgtat ttagaagaag aaaaataggg tttatatttc aaagctataa tttaattcca
    33361 tctcttaatg tatgggaaaa tgtagtgttg ccaatcggtt tagatggtag agaggtagat
    33421 gaacctttta taaaagagct tttaaaatca ttaggacttg aaaataaaca cgacgtttta
    33481 ccaaatacat tatcaggtgg tcaacaacaa agggtggcaa tagcaagagc attagctaca
    33541 agaccagcta taatacttgc tgatgaacca actggaaacc tagattctaa aacatctgat
    33601 gaggttatga gtatattaaa atctatgtct aagaagtaca gtcaaactct agttatgata
    33661 actcatgatg acagtatagc ccaaatggct gatagagtta tattcataga agatggcagg
    33721 gtgtcaaagg ttggtgataa aaatgattaa tactactaaa atagcaaaaa gtaatttaaa
    33781 gcaaaataaa tcaaaaagta ttctaataat aattactata atactttcaa ccttaatgct
    33841 ttcttctatt ggaatttata ttgttgatgc aggagcatat caaaaagaaa atactataaa
    33901 gtattcaggt aattatcaag gtatattagc aaatgtagac gaaaaacaag ctgatatatt
    33961 gagtaatcat gcagatgttg aattaactgg taaaatgaat ggagtaggtg ttgaaaaact
    34021 ggaagatgat tcaaatattt ctctagcata catgaatgaa gatgctttga aattaaatag
    34081 ttttgaattt atcaaaggaa aattgccatc aaaagagaat gaaattgtgt tagatagtgg
    34141 agctttaaag gcacttggtt ataaaaataa agatttaggt gaaaagataa aaatatctta
    34201 taatgactat aaaagtgata aaaaaattga aaaagagttc attataagtg gaatcttaaa
    34261 aactagcgaa atatctgaag caggtaaata ttattatgct attatatcag aatcatatat
    34321 gagaaatact agagatatgt cacaagaaga ttttaatata tatgtgaaat taaatgataa
    34381 aagtaattta tcgatagaac aagcaaaatc aaaattaaat aagattgcta atgatgtagg
    34441 attggacact attaatgtag ctgtaaatga aaactatata aatgctttaa aaccagatat
    34501 ggaaacaatt atgggtggag tatttgtagg gttagtaatt gtattgtcta gtattttagt
    34561 aatatacaat atattttata tttcaatagt tacaaaggtt caagaatttg gaaagcttag
    34621 agcaattggt gcaactaaaa aacaagtaaa aaatatagta tttaaggaag ggttcatatt
    34681 agccggaata gcaataccaa taggaataat tttaggctat gtacttgcaa atattataat
    34741 aaaatcattt atggatatag atgtaaaatc atcacgatta ccagttatat tatcggttgt
    34801 ggtaataagc tttatatctg ttgttttatc acttttaaaa ccaatgaaag tagcatcaaa
    34861 agtatccatt gttgacgcag taagatatac aggaaataaa ataagtaata agagtaagag
    34921 aaaaggatat aaaaatataa atctaaagag attatcacat gccaatttag aaagaaataa
    34981 aaaacgtact tacatgacat tggcatcact tatacttagt ggaactatat ttataacagt
    35041 atcaactgcc ttagaaagtt ttgatgctga gaaaatggca agagaacact ttccttatga
    35101 tatagaagtt agactatctg gttatgaaat gaacagtgat aaaaatccta aagataattt
    35161 gaatatttta caaatggata atcctctaag taaagatttt tttaatcaaa taaagaacat
    35221 ggaaggaatt aaaagaatag aatctgcaag aagtgtaaaa attggtatgg aggactatga
    35281 tgttgaattt aagtatgacc ttttacaaag tataaatgaa aatgatgtaa aatcattaaa
    35341 caagaatata atagatggaa aaataaatct tgaaagactt caaactggag atgaaatagt
    35401 aattactcat gttgatacag caaaagaaat gggagtaaaa gcaggagata aaataagatt
    35461 aactttatat gatggagata aaaagataaa aaaagaattt aaagttcaag caatagctat
    35521 gggggttcca agttttggta tagggaagga ttttattgat agaactttga aatacgattc
    35581 aactagtagc ttgggaatat atacagaaga aggaaagtat caagaaatca aagatagtat
    35641 caagaagatt gctaaaaata atggattttt agagactgat tttatagata gtagaattga
    35701 gtcaaataag gctactattt catttataaa gataatggga tacacgctaa caggcataat
    35761 aggtgtgatt ggatttatga atcttgtcaa cacaatgata actagcatag ttactagaaa
    35821 aaaagaactt ggtatgcttc aagctattgg tcttactaat aaacaattag tgaaaatgct
    35881 taattcagag gcaatgtatt acacttctgg aatgatgata ggaagtattc tatttggagg
    35941 tatattaggg tatattgctg ttatatttct aaaaaagact ggattatctt atgcaacata
    36001 ttctttgcca atagtaccaa tcttgttgat gattgtgtgt attcttatag ctcaatttat
    36061 aacaacttat ttgataggac gaagttttaa taaagaatct cttattgata gagtaagata
    36121 cagtgaataa aatacaaata tttatggtta gtttaactag caaaaaacat ataaaaaaat
    36181 atagttaaag agattcataa ttaaataggt taaagcatag tacagagcaa acaatttaag
    36241 cagatgtttg ctctgtattt tattatgtaa tcaataatat atctaatata ctgatattat
    36301 tcatgttaga aaatgtaccg gtacaattct aaaaaagtat aaaattgtac catataaaat
    36361 gaaaattttg tataataatc gtatagaaaa taaataggag ctgatatata tgaataagaa
    36421 gattaattat gcaccaggac caactgaaac tagggaaaat gtaagattag caagagcaga
    36481 aaaaacaaca aatccagata tagatattga ttttgttgaa ttttataaaa agacatgcga
    36541 gaagtttggt aatataattg gaacaaaaaa cgatgtttac atattgagtg gtgaaggaat
    36601 attaggtctt gaagcagcat gtgcatcact tacagaaaaa ggagatagag tacttgtaat
    36661 tgacaatggt atatatggag aaggttttaa agattttgta acaatgtatg gtggggagta
    36721 tgtgcttttt tcaagtgaat atactaaaag tatagatatt gatgaattga aagcattttt
    36781 agatgaggat agtaatttta aatatgcaac agtagtgcat tgtgatactc caactggcgt
    36841 attaaatgat gtaagcaaaa tatgtccatt acttaaagaa tatggaatat taactgtagt
    36901 tgattctgta gcaggtatgg taggagaaag attaagtgta gatgaatcta aaatagatat
    36961 aatattaggt ggttcacaaa aagctatttc tgcaccagca ggacttacta tagtcggaat
    37021 aagtcaagat gcaaaaaatt gtataaaaaa tagaaaaact gatgtaatag gtttttattg
    37081 taatttaaat atatgggaag gctattatga aaaaaaatac ttcccttata caatgccaat
    37141 aagtgacata atggggcttg ataaagcact tgataatata ttagaagaag gaatagaaaa
    37201 tgtattaaat agacatgaaa agatagctta tagtgttaga aaatctattg aggaatatgg
    37261 tctagaacta tttttagaag aaggatattc taatacagtc acagctataa aaattcctga
    37321 aagtatagga gctttagaac ttactgatta tatgcttaaa aattataata cacttgttgc
    37381 aacatcacta aatcaatata tggatactat tttaagaata ggtcatatgg gagagaatgc
    37441 aaatctaaat aaaatagaac atgtactaaa tgttttggat aaaagtttaa gtgcattagg
    37501 attcaaagga aatggaaatt tgttaaattt atttaataag tattattttt aatagatagg
    37561 gggatatagt atggaagtta cagctaagca aaaatttaca acgaaagaca tggtggaaac
    37621 ctcactatta atagcattag tatttattgc aactaaattt ataaacataa gattaccaat
    37681 atctataaat ggtgggcttg tgcatttagg aactgcaatg ttgttcatat cagcaatagt
    37741 atttggaagt aaaaaaggtg ctttatcagg agctattggt atgtcacttt ttgatttgat
    37801 atctgggtgg actttatggg caccgtttac ctttatagtt agaggagtta tgggttattt
    37861 acttggaaag attgcatggg caaatggtaa taatgggaat aattttttaa tcaatgtaat
    37921 aggaatatgt gtttcatcaa tttggatgct atttggatat tatgtaacag aagttatact
    37981 ttatggtaat tttatagtac ctcttacttc aataccaggt aatttaatgc aagtattgat
    38041 aggtcttata atagcgcttc ctatttctaa ggtattgaaa aaatgtataa aataaatata
    38101 aagtgaacta aaatttatga tagcataaat tatactgtat aatattagga tggagtacat
    38161 aattaaaaaa ttttagtata gaggttagtt cacaatgtta tcagttacaa gatattcaat
    38221 caaaaatgtt cgtttaaagc cgtgggtaaa gtttatttgg tattttgaga caaaatcaaa
    38281 tatactgtta aataataagt tacttccgac agacagtata gatattattc tcaatctttc
    38341 tgatgttatg gaatataaaa ttgaaaatca agactatatt gctagtaata ttcattttaa
    38401 tggaataagg gataaacatg gatttattat tcaacatgga aatatacgtg ttatagggat
    38461 ttctttttat ccatttggtc tctatccatt tttaaagata ccaatttcag agtttaacag
    38521 acaaattgtt gacctagaag cagtatcaaa actatttgca aaaaaattag aagagtcatt
    38581 aaatccaaca aaagcaatag aaaaagttgt tttatgttta gaagaagtat tattatccat
    38641 attagaagaa gatttaattt caaataaata tgtaaagctt ctaaattcat ttatatatgt
    38701 taatagatat agcaatataa aagcattttg tgatgataca aatataaata ttaaaacatt
    38761 ggaaagaatc tgtttaaaat atacagggta tacacctaag attttaaaga gaatttacag
    38821 atttaaaatg gcaagcaatc aacttattta taattataaa gatgatgaac tttttgattt
    38881 tatttatgaa aatgagtact atgaccaagc acactttata aaagaattta aaaaattttc
    38941 agggacgtca ccaattaaat ttagtggtga gaataagacg attaaagaaa atataaagta
    39001 cagctattta taattatact gtcgattttt tacaatacct ccaatttatt ttttgatatg
    39061 atataggaaa ataaattgga ggttttacat atgaaaatta ttacagaaat agttgagttt
    39121 aatgtggaag aaaatttatc aaaagaagag tttatagaaa ttgtaaatga cttagaatta
    39181 aaatttcatt ctattcaatc tggttttatt gatactgagc ttttatatga tgaaaagtct
    39241 caaaagtgga ttatgataca acattgggaa tctatagaag acctcaagag tgtttctagt
    39301 aaaatgttta aagattcttc aactgagaaa tttagaaaag ctttaaagaa tcaaactgtt
    39361 aagatgaata taataccaca aattaaaagt tggaaattat agaactgaat gtaaaagata
    39421 gttaaatagt atcaaactaa attagccaaa taacattttg tatttaaatt gttatttggc
    39481 ttttagtgtt tattttaata tgagtgttat atactagaaa aaccaacaaa ctgttagagt
    39541 gtggattatc ataatgtcat attcgggagc taaaaggctt aatactgatt tgccaattgg
    39601 tcaatccatg catgtccatc tggatatgct gttattaaag catttacaag ccaatccctt
    39661 tgctcttgtg ataatagtgg gactgttaca tcgaagtctt tttgataacc aagcctatct
    39721 atgtatattg atttgcaaaa caaaaccact tcgggagcca tatatgggat attatctgat
    39781 gttatcagtc tggccttatc catatctctt ataattcttt gattttggga aaaaagaaat
    39841 tttccatctt tctgtggatt atatatcaat tcaataaaat cgaactgtaa ctgttcgtca
    39901 cactgtatat tcattttata gttatcatcc tcgacttttt ttaattcaat aaatgagcaa
    39961 tcaggcttaa tcgcaaaaac acaaatttgc tctatatgtt gcttgttaag gtcgataata
    40021 ggtcgtaata aataattacc caagggctca tatatatccc atccttgatt aagcatatac
    40081 tgaatgctct tttctttatc tgtgtcagaa atccatatgt caatatcact gtgtgggcga
    40141 atatgcatat tcgcaaataa ttctagggca tatccaccac atattgaata tgaaaagcca
    40201 caatcattaa aaaagtttac acaatcttta attagtttgt tcatagcaac ctccttttgc
    40261 atgtactgaa ataaattaaa ttttttaaaa taccaactaa atgtttgttt tgtgtaaata
    40321 taaaactgcc gatattaagg cagttttata tttactattt agaaaattta ttaacaaatt
    40381 tttgatattc acacattatc ttatcattca taaaagaaat acccataaaa gcagcttgct
    40441 caccaaacaa agtaaaacgt tctatcatct gttgtggatt acctggataa actgttggaa
    40501 tacaccatgt gtttagaaga aaaccaaaca tttgagcagc aatctctgga tttttaactt
    40561 ccatagaatt atcttgattt ccttcttcaa tcaatgcttt tatccaaggc acaatatcat
    40621 ttataatctc atcaaactgc gtagctaaat acttaggatt acgcaatagt gatgatgaca
    40681 ttaaaaaaat gtcttgttta ttgtgctgtt cataagtatg aagcaaccta tattgtaatt
    40741 tttctaatgc ggtctttcct tcatcatttt ttacctcata cccataaaat ccatcattat
    40801 acaaccgact tgtgattgcc ttaattattt cgtcttttga tttaaagtga tgataaatag
    40861 cacctcttga aaggccatct aaattatcta caatgtcttg aatcgttgtc tgctcatacc
    40921 ctttttctaa gaataactta tgtgaaatat caagtatttt ttgtatagta ttatcaggtt
    40981 ctttttttct tcccaaaaaa ctcactcctc gaaacaatca tttggtatgt ttatcttagc
    41041 aaatacataa tgatttgtca atgtgtcttt ttagcatgtt gaacaggtat attgccatgc
    41101 tttcattatt ttattttttt gccttatact ggactatatt tgcagtttcc taatatattt
    41161 ggctcatatt taaataccat tatctaaaag cactattgct gtacgagagg caataggcat
    41221 tttagaagaa ggaggagttg agctaaagta tacatcataa tctcttaagt tttctttttt
    41281 tgtgtttcct ccaaactcta ccatatcagt atttaatatc gttttgtagt ttgtattttg
    41341 aaactttgaa atatcaactg attgtattag attagggtgg aaattaaata taaataaata
    41401 tttgccctta ctgtacacca aaatcttttt atcattatcc atatgtataa gttcatgact
    41461 attttggata aaaatatcac tgtattttgt aagttcgagc atagatttat caaaatttaa
    41521 taattgttta tactttaagt tgttatcttc tgaaaggctc cattgtcttc tggcatactt
    41581 atagctccaa ttgttgcctt ctcgtggaaa atcaatccac tcaggatgtc caaattcatt
    41641 tcccataaaa tttagatatc cttcacctgc tagagaaaaa gttattaatt taattaattt
    41701 aataagagat attgctctat tgataacatg gttatttgag tttatttcca tattccagta
    41761 catttccttg tcagctagcc aaaaaataat tgttttatcg ccaactaatg cttggtcatg
    41821 ggattcacaa tatcctatat ttttttctcc aggtcgtcta gtagtaagtt cataccacat
    41881 tttgcccaaa tcccagtctt catcagataa gttagagata gtttttatcc aaaaatcagg
    41941 tactcccatt gcgagtctat agtcaaatcc aattccgccg tcttttatag ggatacacat
    42001 accaggcatt ccactcatat cttctgcaat acttatcgaa ttaggtttaa tttctttcat
    42061 taattcattt gcaaattgga gatatgttat agcttcaata tctgtattca tactaaaata
    42121 tttttcataa ctatcaaatg aaactccaag accatggtta tgatataaca ttgaagttac
    42181 accatcaaac ctaaagccat caaaatggta ctcatttaac caaaatttta tatttgaaag
    42241 caaaaaatgt attacttcag gtttaccata gttaaaaagt tttgtacccc aagcaggatg
    42301 atttcccttt gaacctgagt ggaaaaattg atgttcactt ccatcaaact catttattcc
    42361 ttctaaagta ttctttacag catgagaatg aactaaatct aataaaacag caatccccat
    42421 tgaatgagca gtgtttatga ggttttttaa gtcttcagga gtaccaaacc tagatgagat
    42481 tgcataaaag tttgaaactt ggtatccaaa agatgcataa tatggatgtt ccataatagc
    42541 cataagctga atggtattgt atccagcttt ttttatctta ggaagtattt tttcagtaaa
    42601 ttcattataa gttccaatag attcgctttc agtagacata cctatatgac attcatagat
    42661 tagtggagaa gttatgtttt ttaagtcaaa atcattgtca gtccagataa atggagtttt
    42721 tggttgccat atttgtccat taaatccacc aaaatctttt tgaattactc tttttatata
    42781 taatggtata cggtcaaatg tttttccatt tgcagtaact tgtaccttta cctctgattt
    42841 gtgaggtaag ctattttgac caggaataaa tatttcccaa ttaccagacc caatttttct
    42901 tagaggatga gattttctat tccaattatt aaagtcacca attagtgaca aactatctgc
    42961 atttggagcc cattccctat agaagaatcc atcatttgtc tgatgaaatc catagtaaag
    43021 atgagcattt gcaaaagaac taaaatcttt atagtttttc aatgtttttg atttcatatc
    43081 ctcataatgt ttcattcgct tttttatatc agattcaaat ggttgtaggt atacatcaat
    43141 atctaaaatt ttaaatcttt tttgactttt cattgtattt ctcctccata ataaatatta
    43201 catctaaaaa ataatattta tctcatacta tcttctctgt ataattgata tatattatat
    43261 aatcctaata aaaagcttaa gccaccaatg attaatttca taatttacct ccctagtatt
    43321 tatacttata tatcgtattc atcaaaattt aaaagtttgt ttaaaactct tttaataata
    43381 tcataatcaa agcctttata ggaaagatgt tgagaaattt tttgatatat tttcttttta
    43441 tcttctttct ttactctttc ataccttttt tttgctagat acatagcatt ttcaaattca
    43501 gtgtccttgt ctaattcaga tactgcttca ttgattgtac ttctattgat acctttatta
    43561 tataaattct gcttaattct gtttttgcca catttattta ggttaacatt tgtgttaaca
    43621 attttttgag ctaatacact gtcatttacg agattataac ttttaagaaa atctatgacc
    43681 ctctcaatta tattttcatc aaattctgat gatagttttt cttttaattt tttttcagat
    43741 tggtcagcat gagataaaat atttaatgct ttatttttag cttttatata catttcatca
    43801 tctaaaatag gttttaattc ttcttcattt atttcattcc ctttttttaa attaaatgta
    43861 taaaccagtt ctttaaatat agcaataaaa aatttatcat ctacataaat attaactcta
    43921 tcatcatttc ttttttgttg ttctattttt gtaattatac cca
//
LOCUS       DAYATN010000018        43171 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.018, whole genome shotgun sequence.
ACCESSION   DAYATN010000018 DAYATN010000000
VERSION     DAYATN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43171
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.018"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..1876)
                     /locus_tag="ACGI8A_000975"
     CDS             complement(<1..1876)
                     /locus_tag="ACGI8A_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178983.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKLVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     T"
     gene            complement(2059..3534)
                     /gene="spoIVA"
                     /locus_tag="ACGI8A_000976"
     CDS             complement(2059..3534)
                     /gene="spoIVA"
                     /locus_tag="ACGI8A_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HFH0178984.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3706..5001)
                     /locus_tag="ACGI8A_000977"
     CDS             complement(3706..5001)
                     /locus_tag="ACGI8A_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFH0178985.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDESGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(5185..6630)
                     /locus_tag="ACGI8A_000978"
     CDS             complement(5185..6630)
                     /locus_tag="ACGI8A_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0178986.1"
                     /translation="MSLNKFEIGGITVKKKIGFWDLVFMNISALFGIRWIAKSTASSF
                     GLGLGSIPVWLLFAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLN
                     WTSKIFWYSSFLTFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGK
                     FFTNTGALGSTIPAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAII
                     FALSGAETTANFITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAAST
                     GILDALAKVAQDLGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQ
                     LTVTNKYNIPSNAVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFT
                     SYIRLRKTRPNEERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYE
                     IEMIGGGLFVIITGLMLWKNYEKKISNNSGC"
     gene            complement(6646..7785)
                     /locus_tag="ACGI8A_000979"
     CDS             complement(6646..7785)
                     /locus_tag="ACGI8A_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFH0178987.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            8104..8754
                     /locus_tag="ACGI8A_000980"
     CDS             8104..8754
                     /locus_tag="ACGI8A_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0178988.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(8911..9930)
                     /locus_tag="ACGI8A_000981"
     CDS             complement(8911..9930)
                     /locus_tag="ACGI8A_000981"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFH0178989.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(9961..10575)
                     /gene="plsY"
                     /locus_tag="ACGI8A_000982"
     CDS             complement(9961..10575)
                     /gene="plsY"
                     /locus_tag="ACGI8A_000982"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFH0178990.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(10610..11935)
                     /gene="der"
                     /locus_tag="ACGI8A_000983"
     CDS             complement(10610..11935)
                     /gene="der"
                     /locus_tag="ACGI8A_000983"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFH0178991.1"
                     /translation="MSISRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRI
                     FAEVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAA
                     DKEIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLD
                     EIVENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAID
                     TYFEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTE
                     QDTKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAK
                     TNQRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTD
                     IRPPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(11963..13291)
                     /locus_tag="ACGI8A_000984"
     CDS             complement(11963..13291)
                     /locus_tag="ACGI8A_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HFH0178992.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(13529..13729)
                     /locus_tag="ACGI8A_000985"
     CDS             complement(13529..13729)
                     /locus_tag="ACGI8A_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0178993.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(13731..14663)
                     /locus_tag="ACGI8A_000986"
     CDS             complement(13731..14663)
                     /locus_tag="ACGI8A_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HFH0178994.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(14708..15640)
                     /locus_tag="ACGI8A_000987"
     CDS             complement(14708..15640)
                     /locus_tag="ACGI8A_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HFH0178995.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(15695..16984)
                     /locus_tag="ACGI8A_000988"
     CDS             complement(15695..16984)
                     /locus_tag="ACGI8A_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0178996.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(17013..17705)
                     /locus_tag="ACGI8A_000989"
     CDS             complement(17013..17705)
                     /locus_tag="ACGI8A_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0178997.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(17720..18481)
                     /gene="pgeF"
                     /locus_tag="ACGI8A_000990"
     CDS             complement(17720..18481)
                     /gene="pgeF"
                     /locus_tag="ACGI8A_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFH0178998.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(18478..18939)
                     /gene="nrdR"
                     /locus_tag="ACGI8A_000991"
     CDS             complement(18478..18939)
                     /gene="nrdR"
                     /locus_tag="ACGI8A_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFH0178999.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(18961..19224)
                     /locus_tag="ACGI8A_000992"
     CDS             complement(18961..19224)
                     /locus_tag="ACGI8A_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HFH0179000.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(19328..20101)
                     /gene="sigG"
                     /locus_tag="ACGI8A_000993"
     CDS             complement(19328..20101)
                     /gene="sigG"
                     /locus_tag="ACGI8A_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HFH0179001.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(20193..20915)
                     /gene="sigE"
                     /locus_tag="ACGI8A_000994"
     CDS             complement(20193..20915)
                     /gene="sigE"
                     /locus_tag="ACGI8A_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HFH0179002.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(20940..21785)
                     /locus_tag="ACGI8A_000995"
     CDS             complement(20940..21785)
                     /locus_tag="ACGI8A_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HFH0179003.1"
                     /translation="MVYIEYYVIENLVINYIIISCTSILTKRYNPKKNKLLGASLGGA
                     YSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLVNIFISGSTY
                     FIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTININLLEKSFCC
                     KALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQEIIGSLDDK
                     TSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDREYGAIMNPN
                     ILPGF"
     gene            complement(22102..23376)
                     /locus_tag="ACGI8A_000996"
     CDS             complement(22102..23376)
                     /locus_tag="ACGI8A_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFH0179004.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(23660..24817)
                     /gene="ftsZ"
                     /locus_tag="ACGI8A_000997"
     CDS             complement(23660..24817)
                     /gene="ftsZ"
                     /locus_tag="ACGI8A_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFH0179005.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(25007..25360)
                     /locus_tag="ACGI8A_000998"
     CDS             complement(25007..25360)
                     /locus_tag="ACGI8A_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HFH0179006.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(25459..26133)
                     /locus_tag="ACGI8A_000999"
     CDS             complement(25459..26133)
                     /locus_tag="ACGI8A_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFH0179007.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VDLFAK"
     gene            complement(26138..26836)
                     /locus_tag="ACGI8A_001000"
     CDS             complement(26138..26836)
                     /locus_tag="ACGI8A_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFH0179008.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(26859..27599)
                     /locus_tag="ACGI8A_001001"
     CDS             complement(26859..27599)
                     /locus_tag="ACGI8A_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HFH0179009.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(27869..29095)
                     /gene="murG"
                     /locus_tag="ACGI8A_001002"
     CDS             complement(27869..29095)
                     /gene="murG"
                     /locus_tag="ACGI8A_001002"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFH0179010.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(29113..30243)
                     /gene="spoVE"
                     /locus_tag="ACGI8A_001003"
     CDS             complement(29113..30243)
                     /gene="spoVE"
                     /locus_tag="ACGI8A_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HFH0179011.1"
                     /translation="MPKENLKKQIDIRVKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(30295..31650)
                     /gene="murD"
                     /locus_tag="ACGI8A_001004"
     CDS             complement(30295..31650)
                     /gene="murD"
                     /locus_tag="ACGI8A_001004"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFH0179012.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSDNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(31669..32637)
                     /gene="mraY"
                     /locus_tag="ACGI8A_001005"
     CDS             complement(31669..32637)
                     /gene="mraY"
                     /locus_tag="ACGI8A_001005"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFH0179013.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(32678..34051)
                     /gene="murF"
                     /locus_tag="ACGI8A_001006"
     CDS             complement(32678..34051)
                     /gene="murF"
                     /locus_tag="ACGI8A_001006"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFH0179014.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVIDGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(34218..36197)
                     /locus_tag="ACGI8A_001007"
     CDS             complement(34218..36197)
                     /locus_tag="ACGI8A_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HFH0179015.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(36209..36556)
                     /locus_tag="ACGI8A_001008"
     CDS             complement(36209..36556)
                     /locus_tag="ACGI8A_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179016.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(37037..37972)
                     /gene="rsmH"
                     /locus_tag="ACGI8A_001009"
     CDS             complement(37037..37972)
                     /gene="rsmH"
                     /locus_tag="ACGI8A_001009"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFH0179017.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(38002..38748)
                     /gene="lgt"
                     /locus_tag="ACGI8A_001010"
     CDS             complement(38002..38748)
                     /gene="lgt"
                     /locus_tag="ACGI8A_001010"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFH0179018.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(39142..40428)
                     /locus_tag="ACGI8A_001011"
     CDS             complement(39142..40428)
                     /locus_tag="ACGI8A_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFH0179019.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(40443..41708)
                     /locus_tag="ACGI8A_001012"
     CDS             complement(40443..41708)
                     /locus_tag="ACGI8A_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFH0179020.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            42024..43121
                     /gene="ychF"
                     /locus_tag="ACGI8A_001013"
     CDS             42024..43121
                     /gene="ychF"
                     /locus_tag="ACGI8A_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFH0179021.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        13453 a         7954 c         4532 g        17232 t
ORIGIN      
        1 gtgttagtat acaaaataaa aaaataatat ctttttttag attatattct ttagattcta
       61 atttataatt attatttatt attccatcat aatcacttat atttgactta tttttatttg
      121 tatcttgatt tttgttagaa atactttccc tataaggaaa agaatgttca ttggtaccaa
      181 atactaattt tgagcttaaa tcataattat catcattttc tgtaattgaa tttttattgt
      241 aaaatccttt ttctttatca gaattatttt ctttattacc gtctttaggt atatcatgag
      301 gatttttatt ttcattgcta tcagtacttg attctacatc atctttagga gtatttggtt
      361 gtttctcatc agaattatca ttttcattat tattattttc attattattg ttttgattgt
      421 tattactatc attattttca tttccattat tcccatcttg attgttatca tcctcatctc
      481 tatccgggtc gtctctacct cctcctccac ggtcaccatc aatatcatca ttttcccgtt
      541 catcatctgg tgtctcttgg ttgtctccgc ctcccattat aaatcctggt ttaaaaacta
      601 tatttgaata cccttctttt aatcctccta ccacttttac tctaaaacaa cgcaacttaa
      661 aatcttcaaa aaaaagtata gcttgatctc taatatcctc ctgttcttca gatatccaat
      721 tgattccatc gcttgaatat tcaacaaaaa ccttacttgc actatacggc atatcataaa
      781 ataaaacagc tacatatcca ttttttgtat tttgaaattc cagttccaaa ttgtctattg
      841 gttttttctc tataacagat acattcaaaa taggaactac atcatcattt attataattt
      901 tttctttatt taacatttct gttttaaact ttcctgttat attaaaattt tctccctttt
      961 ccaacttttc ataataatca gtaaaatccc attctacatc tatatcaagt cgattaccac
     1021 tagatgtttc taataccata tatttgggaa attttatatt ttctatttta tcatctttac
     1081 ttacttttat ttcatcacta aatacaccaa ttcccctatg acaacatttc acaatatcat
     1141 agccagaatc ttcagctata gagggtataa gctcattata atcatcaatt atataaactt
     1201 tcttactttc tgatataatt gattgtgcca acttattatt atcttttagt aacccacttt
     1261 cattgttgct actatatgct tttattgaag tttcttctat ctcaacatca cctttagaat
     1321 aaacacctat agcatgttct ccattcactt caatatcctt atttagtatt ctaatatcat
     1381 tttcagtata aattcctatt gcatctttcc catttgcctt tattttttca acagattcgt
     1441 aggatgattt aatatgtaag tctcctcctt cttcaacgta taaggctgtc ccatcagtag
     1501 caattatgtt gtttatacca atacttatac ttccaccatt ttctatatgt attactcctt
     1561 cttttcctcc ttcacctatt atattcaatt cattatcatc aataataaat ctcccatgat
     1621 tttttatcaa tattttatat ttgttggtat ctattgtaaa attactgtca taaggaatat
     1681 aaaatctaaa ctcttcatct attaccatat cacaagaaag agtataaact atttcttctt
     1741 tattattttt attttcttga acccaccttt caaaatcttc aagacaattt tcctcttgtc
     1801 caaaaacttt atgtggaaaa cttataaaac acaaaactat tatactcata aaaataaata
     1861 tctcatactt tttcatctta cactcctaga aattttttag ctttgaaaca atctgtcgaa
     1921 acatacactt cacccactat aatttttcca caattatcaa ttattgtcaa tttataaaag
     1981 tatctttatt aattgttata aataaaaagc tacttactaa agtaaacaga cgtttcactt
     2041 taataagtag ctatattttt ataacaaaat agttataata ttagaacttc cttcatttac
     2101 aattttttgt aaagtctttt gcattttaac tcttatctct tctggcatag tatataattt
     2161 actttgtaat tgctctttta ctaagtcatg tagtgattta ccaaacatat ttgattccca
     2221 taaatctgct ggttgctctt caaatacttc cataagatat tttatcatct cttcaccttg
     2281 attttgattt ccaactattg gagaaacctc tgtagatata tctgctttta taatatgaag
     2341 tgatggtgca ttggctttta acttgatacc atattgtttt ccttgtttca ttatttctgg
     2401 ttcttccaga ctaagttctt ctaaggaagg agctactact ccatatcctt ttatcttggc
     2461 atctattaaa gcactttcta ttttatcata ctcattttta actttagata gtctagttat
     2521 cagactaagc agttgataat ctccttcaat tttaaatcca cttttttctt caaggacatt
     2581 atagaataaa tcttgctttg tctgtaaatc aatatttata acaccttctc ctagctctac
     2641 attatcaaca cctgtatctt ccaaaaactc taactcagaa aatccttgta taataccttc
     2701 tatatccctt attttaccta tatctataat actttgtttt aatgttgtta ttatgctact
     2761 ttttatccaa tgatttcttt caagaccttc aacccatttt ggtaagttga ttcttatttc
     2821 tgtaagtgga aaatcatata atacagcttc cataacctcc tctatatctt cttcttccat
     2881 ttcaaccaca ttcattggaa gaactggaac ttcatacttt tcttccaatt cacttctaag
     2941 catactcgtt tcctcacttt ttggacttaa cgtatttaaa accactgcaa atggtttttt
     3001 aagatttttc aattcttgta taactctctc ttctggttct acatagcttt ttctgtcaat
     3061 tccagtaact gaaccatctg tcaagacaac tatccctatt gtagaatggt cttttataac
     3121 ttttttagtt cctatctctg ctgccttttc aaatgtcata gcttcttgtg accaaggagt
     3181 ggatactaat ctttgttttc caccttcctc atgccctaaa gctccttcaa ctatgtatcc
     3241 aacacaatct accattctta cttttaaaga tactgtgtct tttattttta tttctacacc
     3301 atctgctggt acaaattttg gttctactgt cattatcgtt ttacctgaac cactttgggg
     3361 tatctcatct cttgttctat cctttttaaa ttcattgtct atattaggta taaccaactt
     3421 ttccataaat tttcttataa aagttgattt tcctgttctt acaggtccaa ctactccaat
     3481 atatatatcc ccctgagtcc ttttggatat atcttcgtat atgttattat tcatattatt
     3541 cctcctatac atctaatctt attaaattat atttagagtt aagggttaat attcttaaaa
     3601 tagaaaaata aagaccagca atattaattt aatttatata tagaaatttg taaatttcaa
     3661 tgtaaattaa attaatattg ctggtccaaa tagtagtgta ttatattaac tgactttagt
     3721 agtattttca tttgaattat tattcatctc aataagttct ttacgtcttt cttttgtcat
     3781 gttcatccat ataaatgtaa acaccaatcc aattatggct actatagtaa gaactatgaa
     3841 gtagtaccta tatccagtta cacccggaaa tgcatccaat atcttccctg ctgctaaagg
     3901 acaaagtact tctggaagat atcctaacgt agcaactatt cctatagcag ttcctgatat
     3961 tgataatgaa taattacctt cttcaagtaa cgaatagtgt aaaccttgaa ttgcatacat
     4021 agatacatat attgcaacac atccaatcaa tagtatataa atcatagatg attttgttgg
     4081 tgtaaatatt acacctaaaa gacctgcaat cataagtata aatccataag atataatctt
     4141 tgatgaacca actttatctc caagtaatcc agaagcagct gatgctactg gtctacaata
     4201 ctgagctaat attgagattg cagatgcaaa caccacagta gctccaaatg cttcagttga
     4261 ataaggcgta aaataataaa atgacatatt agctgaatat gaacaaaaca ttattattgt
     4321 tattatccat gtatgaggca tttttattac tgcaaaaaag gcttctttat caaattttgc
     4381 tgatacctct ttttcattat cctttaattt gaaaaatact aatactccaa ctattacatt
     4441 tattgctgag tataatacta taatagctgt aagaccagat tcatcactta cttttgatga
     4501 aatataccca aatagtatca atactagaga tagatgaatt gcattggtaa ttccacgacc
     4561 actttccata aatccaaaag cttttccttg ttcattttca gatgctaaca ttctaacagc
     4621 ttttacaaga gctggccaaa aagtaagtat tgttgttatt ccccataagg catggattaa
     4681 aaacacaaca atatatggag gataaagtgt taatacaaga ccagatattc ctgtaataat
     4741 taaagaacct gaaagcaatt ttctggctga aatacggtct gctaaaaaac caccaaaaag
     4801 gtatgatacc attgcaagta gaccataaat gcttcctaag cttcccattt gtgtatttgt
     4861 aagattaaat gcttgtgcaa aagtatcata ataataattc tttaaatatg gtaaagcata
     4921 tataaatgct cctgctacaa agagtattat caagactttg aaatttttcc aaatgcttgg
     4981 tttatttact tcaactgaca tacttcctcc ttatgtaaat acctgtaaat tttcataata
     5041 attaattatt gcttatttta ttgtttattc aatatttagg aatatttatt aatatattag
     5101 gttaatctat caataattta tggtgagtta tgagctaaaa gtaaactctt ttagctcacc
     5161 ataataatta tatttttatt tatactaaca tccagagtta ttagatatct ttttttcata
     5221 gttcttccat agcattaaac ctgttattat aacaaaaaga cctcctccta tcatttcaat
     5281 ctcatagatt atattatctt ttaaagtctc cataactggt gccgttgaga aaagtatgcc
     5341 aactacagtt actacaagta ccattctggc aatattgata caaactgaat cactttttgc
     5401 aattgaatat ggtctttctt catttggtct tgtctttcta agtctaatat aagatgtaaa
     5461 aagcaagaca tatggaaata atgctgttaa tgctgtcata gtaacaagaa cattgtatat
     5521 attatctact gatggtaata agtttgttcc tacaagaagt agactcacta ttatagcctg
     5581 aactattact gcatttgatg gtatattgta tttatttgta acagtcaatt gcttaggaaa
     5641 tacaccttct cttacatttc caaaaagcat ctttactgga cctgctatat aaagtattaa
     5701 tgctcctagt actgataaag ttattccaaa tgctactatt cttataaacc aacttcctat
     5761 acctaaatct tgtgcaactt tagctaatgc atctagtatc cctgtagatg ctgcaatttt
     5821 atctggagga agtatcattg ttattgctac tgaacctaag atatagattc ctcctattaa
     5881 aacagccaca gttaatatag ctttaggaaa gtttttctta gcattatcca tttcggttat
     5941 gaaattagca gtcgtttcag ctcctgataa tgcaaatatt atagcagata tagaaactaa
     6001 tgaattggca tctatttttg gtattatatt ttggattgta tatatcgatg ctgatggagc
     6061 ttttttgaga ataactacag acataaatgc catcactata agtaatatag caggaattgt
     6121 agaacctaat gctcctgtat tcgtaaaaaa ttttccaaat gccatcccac gagtacttac
     6181 taaggacaac agccaaaata ttatgagtga aagtgctaaa ataaacatct tattgtttga
     6241 taaatctgga tttccaagca tatatgcaat atttatcgct aaaaaagtca agaaagaaga
     6301 ataccaaaat atctttgatg tccaatttag ccatgacacc ataaatcccc acttctctcc
     6361 ataagcttgt ttaacccatt ctccaagtcc accatctttt ggatatgttg cagccaactc
     6421 tgcacatata agtgatgcag gaacaaagaa tataaaagca aacaataacc aaactggtat
     6481 agaaccaaga cccaatccaa aactagaggc agtagattta gctatccatc taatcccaaa
     6541 tagtgctgat atattcataa atactaaatc ccaaaatcca attttctttt tcactgtaat
     6601 tcctccaatt tcaaatttat tcagactcat acacaatgta ttgacttaaa tatcaagtat
     6661 aagttcttca acacttcttg gataattagt tataattgta gcaccattct tacctactat
     6721 tgctgtatct gagtgtctaa atcctccaac tccaggtata tatatccctg gctccataga
     6781 aaataccatt ccttcttcta atattaattc attgtcaaat cttaaatatg gctcttcatg
     6841 ttctgataaa cctaaaccat gaccaattct atgatttgaa tataactcta gaccatactt
     6901 tgcaacaaca gctcttgctg cttcatccac cattcttgct ggtataccag cttttattgt
     6961 atctaatcca gcttgttgag cctctactgc aatcttaaac acttcttttt gtctttctgt
     7021 tggtttgcct ataataaaag ttctttcatt ttctgctcta tagttttcat accaaacctg
     7081 tctactatgt attactatat ctcctctttg aagtattcta gtattggaat ctaaatgagg
     7141 ttgtgctgtt ctatcaatcc ctgaacaagt ccagtttgca aatcctatat atgtatcagg
     7201 ataattttca gaaacatatt ttagtaaagc attatcccct gcaacgtcaa actccaattc
     7261 actcatccca actctaacat tttctaaaga cccttttatt cctatatcag ataggtaacc
     7321 tgctatcttc aaaaattcta tttcttttgc atcttttaca tatctcattt caaatatttt
     7381 acttccaaca tctttgatag caaatgactt atcagttata tactctgaaa agcttgcaga
     7441 tactatatct ttttctattc caacagttgt tcctttaggg tatttattta aaattatatc
     7501 taagtaatgt ttatgtgata ttccatgttc tttcatttct ggtatttcat aatacacata
     7561 tacattatca acttttgcaa cttctttagc atgtaattct tctaatccag gtactattaa
     7621 ttctatttta tcttgagtta ctactgttac aattggtcta gaataagtta ttgctttaaa
     7681 tccactaata taaaattgat tgtctggttc aaatattaaa gcaatttcaa tgcttttttc
     7741 attcatgtac tttctcaatc tttctagtct catattattt gacataatgc acctctttct
     7801 taatattcag ataatttatt caatacaaca aggatgaata tattatttta aaattcttaa
     7861 tatttttagt acttgtcgta tttgtatgta aattaatttt atttgtgatt attttatcat
     7921 aatcatgatt attttgtcaa tcatgatttt attttaaaaa attttttata ttctagtgat
     7981 tttaaattat ctaaaatata acgagtatac attgtatttt gtaatattat tcttgattta
     8041 tggtaagttt aatgataaaa tcacgattat ataaactata ttgattattt gtaggtgaat
     8101 aaaatgacaa ataaagaatt acaaaagaaa aggatattga tgtatttcat agaagctgct
     8161 caaaatatta tggaaaacga aggtatagaa aatattacac ttcgtaaggt tgcagatatg
     8221 gctggctata atagttcaac tctatataaa tactttaaaa atttagacca tttaatatca
     8281 tttgcatcta taaaatattt taaagattat aacttgaata tttcaagatg tatagaaaat
     8341 gtcaatgatg aatataagaa atatataatt atgtggaaat tattttgtaa gcattctttt
     8401 gataatgctc aagcttttta ccaggtattt tttaacttgt ctagtgatga acttagctat
     8461 ataacaaaac aatattatga tatgtttcca gaagatttag gtattcatga tagtgacata
     8521 tctttaatga ttacaggaga aagtataaag gaaagaaata agatactatt aaacaatctt
     8581 gtagatactg gatatatacc agctaaagat ttagacataa tcaatgatat aattatttct
     8641 ttttatcaaa atctattatt cttaaaaaag aacacaggaa aaaattctga tgatgatgaa
     8701 ttaactatga aaatgatttc ttctattgaa tacataataa aacatgctaa ataaaattaa
     8761 aatatggctg tctcaaaata gagataagcc ttttttattg ttgaaataat aaaaatatat
     8821 ttaatttcat ataaaaaaga gtgcctcgta aactaaaaag ttcattttga gacaccccta
     8881 aaataaattt attatttatt atacaatagt ttatatatca tcacctaaaa ctacttcttc
     8941 catttcatgc tttttacttc tcatcattag ttctattcca acttctttag gatttgaacc
     9001 tttctttatt acagaatata tagcattagt tataggcata tctatatcta atttttcagc
     9061 aacatcatgt gctacttcag tagcagttat gccttctaca accatcttta cttcttttaa
     9121 agtatcttct aaactcatcc ctttacctat gagtatacca gctcttctat ttctactatg
     9181 catacttgta caagtaacta ttaaatctcc aatacctgaa agacctgcaa aggtagacat
     9241 attagctccc atagcaattc caagtctgct catttcacta atacctcttg tcataagggc
     9301 agcttttgca ttatcaccat accctaatcc gtcacatatc ccagctccaa atgctataat
     9361 attttttaat gctcctccaa gttctactcc aactatgtct ggattcgtat aaactctaag
     9421 tttagggctc ataaataaat cttgtatcat ctgtgctatt tttaaatctt tagaagcaac
     9481 tactacggta gtaggaatat cccttgctac ttcttcagca tgagatggcc cagatagtat
     9541 tacatatgga ttttgtggta attcttcttc acagacttga gataatctaa gcccagtccc
     9601 tttttcaaga cctttagcta cattaacaat aacttgtcct tcttttataa atggttttat
     9661 tttttggcaa gtacttctta cagcctgtga tggaactgct aaaactatta ccttacaatc
     9721 ttttatagtt ttttctatat cagtacttat agttatatta tttggaaata atactcctgg
     9781 taaatagtct gtattttctt tagttctgtt tattttttta gcttgttcct cttttcgagt
     9841 ccacatgctc acatcattac ctttttttgc aagccctaaa gctaatgcac ttccccagct
     9901 tccagtacct aatacacata ctttctccat gtttaacacc acctgagtat aatatattta
     9961 ctattctttc ttttgaccaa tttttctctc tgtcccattt aataatcgct ttatatttgc
    10021 tctgtgatta tatattactg atgcagttaa gaacagagct actattaaac cagctttatt
    10081 ttttaccata atcataaata ttggtgaaca tcctattcct acaacagaac ccaatgaaac
    10141 atactttgtt atagctacca ccaatataaa tactgctaga cacattagtg ttattactgg
    10201 atttacagct agcattgccc ctagtgaagt tgcaacacct ttgcctcctc taaatcctaa
    10261 aaccgctggc caattatgac ctgctacaac acatataact gccagatatc cagctaaaag
    10321 agtatctatt ccaaccaatc ttgctgcaaa ttctgatatt aaaacagcca ttacaccttt
    10381 aagaacatca cctaaaaatg tcattgctcc tgctcttttt ccaagtgtcc taagtacatt
    10441 tgtcgaacct gcattaccag aaccttgagt ccttatatca accccagcaa ttcgctttgc
    10501 aactatgtat gaggtagata tatttcctaa aagatatgca actacagcaa taattatata
    10561 actaaatatc tccactgaat ttcccccctt tagttatatt ttaagatatt tattttttat
    10621 ttttttgtct atactcaaac tttatggaag ttccttcaaa tccaaaattt tctcttatct
    10681 tattctcaag gtatctttga tatgagaagt gagttaaatc cttatcattt ataaacaatg
    10741 taattttagg aggtctaatg tcagtttgag tcccatagta aatttttaat cttctacctt
    10801 tgtcagatgg tggttgattt aacattacag cttctccaat tacttcattc agtgctgaag
    10861 ttgatattct cttagaatgt tcatttgaca catattcaac tgtatctagt atcttgttca
    10921 ttctttgatt agtttttgca gaaacaaata cgattggagc atacatcata aatggaaatt
    10981 tttcttttat atcttttgtg taattgctca tagtcttatt atctttctct attaaatccc
    11041 atttattgat tacaaatatg catccttttc cttcatcatg tgctatacct gcaacctttg
    11101 tatcttgttc tgttacacct tctaaggcat ctattactat caatactaca tctgctctat
    11161 ctacagcact catagccctt attacactgt atttttctat tgtctcatat attttacttt
    11221 ttcttctaag ccctgctgta tctattagaa gaaatttctg tccatttttt tcaaaataag
    11281 tatcaattgc gtctctagta gttcctgcaa ttggacttac aattactctt tcttctccaa
    11341 gtatcttatt gagtatagaa ctttttcctg catttggttt acctgttata gctactctta
    11401 ttatatcttc ttcatactct gtatctaatc ctgctggaaa attttcaact atctcatcta
    11461 ataaatctcc aaatcccata ctattggctc ctgaaactgc aaaaggcgtt ccaaatccca
    11521 actcataaaa atcgtatata ttatcaaatt gactttgact atcaatctta ttaaccacta
    11581 agattactgg tttttttgtc tttctaagca attgtgcaat ttctttatca gcagctgtta
    11641 ttccagcttt accatcaact ataaaaagta ttacatgtga catgtccatt gcaagcatgg
    11701 cttgatttct catttgagat aatattatat cttcgctatc aggttcaatt cctcctgtat
    11761 ccactaatgt aaaatattta tctaaccatt ctacttcagc aaatattcta tctcttgtaa
    11821 ctccaggtgt attttcaact attgatatcc tttttcctgc aaatttatta aatattgtag
    11881 acttaccaac gtttggtctc ccaactacag ctacaactgg cctacttata ctcattttat
    11941 ttctccttcc atcttttgtt ttctatttta aaattttatc cacaaaatct tttccttcat
    12001 tcaaacaagg gataattttt attctcataa gttcttctaa ttgatttaat tcaatattat
    12061 ctaagaaaat ttcttcatct gcttttagca tacttcttgt tatataaaga gcttctccta
    12121 attctttatc ttcaagttgt tcttttaaat ccttagctgt taaaagacct gatacagtta
    12181 ttgtctctcc aaaaaacttg tttttgattt catatacatt tacttgaaga tttttaaact
    12241 tgtccatcat agcatctgcc atactctgta taaattcata tgcagaatga ccagtagcta
    12301 ttgatacttt cttctctctt tttagaatct tctcaggcaa tatatttaga tactcttcaa
    12361 tctctgtttt taacttacgt atcataccta caccatcttc aaactgtaaa aaaccttcat
    12421 attcttcata tccaggcaat tcactattag atagtatata aaattcatct gataaaaatg
    12481 caaatcgagt acctaatttt tcaagatatt tctgttgtat ttgatgtatt tgctctatag
    12541 ttttacttgc gctcttatca ttaaatatat ttaattctac tagattttct ctatgctttg
    12601 taatcccaac tggaactatc gcaacactgt ttacatacgg ataaagttga gctagttctt
    12661 ttattgttct atccaattcc tttccatcat ttatacctgg acataaaaca atttgacagt
    12721 tcatttctat atgagcatct gccaatctct tcattatacc atataatttt cctgcaaatt
    12781 tattgcttat cattttttgt cttaactctg gattagttgt atgcacagat atatttatag
    12841 gacttattct atattttata atattattta tatcttcttc actcatattt gtaagtgtga
    12901 caaaatttcc ttgtaaaaaa gataatctcg aatcatcatc tttaaaataa agagtttctc
    12961 tcataccttc tggcaattgg tctataaaac aaaacataca cttatttcta cagcttttag
    13021 cttggtcaat tatcggattt gtaaattcaa cacccaaatc ttcatcatag tctttttcta
    13081 tctcgtatat gtagacttcg ccatcttttt tttgtatttc tacatctaaa tattcatcac
    13141 ttagtaagaa tctatactct attatatcat gtattggctg ttcattcaca gatataagta
    13201 aatctcctac ctctataccc atttcttctg caatactatc tttataaacc ttacttataa
    13261 tattatttac tttttttaca tttacttcca atattttcac cactcttttg tattcatcaa
    13321 tgcttattat aatgtatttt aatatgcaat ttatttattt attatatttt atgatagcat
    13381 ttttaagcat ttcatgtcat aataacacat tttaattcta atagtcaata taagtaaata
    13441 gctataaaat agattagaac aaataatatt caaatttaag tttcttaaaa atttaaatat
    13501 tatttgttct taaaaaaatt ttataaattt aatgttttac tatgattaac gtcccatttg
    13561 tcatatcatg aaccattcca cctacacact ttgacatcaa caatgcctta ttttgtaagt
    13621 ctttctcatc atttgcataa aatattggac aacctccacc atttttcata ttcttatcaa
    13681 gtgtaactat agctagggta tattcattta aacctatatc cattcccatc tcatgcactt
    13741 cctttctttc ctattttata attttttagt gataagtttt ttcctttaga gcttgaaagt
    13801 attgggcatg actttacagc ttccattact ttttccacat tcttatctac tggtaaataa
    13861 gcaatcatta tactttcatc ctctttattt cttctaggta atggtacgaa tgaaggttct
    13921 ccataatctc catataaacc taaccttgaa tatatattgt atactattgc ttgtctttga
    13981 ccggagtcaa ataatattcc tgcattagca taatccttat ttttaggaat tatttgtatt
    14041 cctacacctc tattaatata tacttctcta tcgcttttta gacctatatt tgttatacct
    14101 tttaaatctc ccacctttag tatagattcg tccacaaaac taatttcagc aggcattaca
    14161 tcagctatgt ctccaatact ttttctagta agaagttttt ttaatataaa ggctaacact
    14221 aatccacaaa ttgaacttat taccaaactc atcccttcac ttattttcgt ttctgcaagt
    14281 attacataat acaatcctat tgttaaaaaa gatgtaacta tacacatata atttcttact
    14341 tcataggttc tagctatctc ttctatgaat gcagctcctc tctttacaag ttgaacttcc
    14401 tctaaatttt gcaatgtatt tcttctattt gctctcactt gtctaaattg ttctgctgct
    14461 agagataaaa aagttataga tgtataagaa cgatttataa gagctggaac caataatgct
    14521 cctaatgaag aagcgacgaa tgctaatata agttgagata gtaaaacatt tggttgcgtt
    14581 ggatactgtt tttggtctaa attcaacatt ataattctag atgttatacc aataataaca
    14641 gcaacaaaaa atactctatc cattaaatca ctcctaaaat atttaatttg aaattttaca
    14701 tgtaaatcta caaagtcttt ctagaaaaat cactctgctt tccttttgat gtttctatga
    14761 taggtgtact tttcactaca tctatcatga catctatatc tttgagtatt ggcatatatg
    14821 gtataaccac agtacttttt tctaaattgg tcttagatat agcaacaata tcaacctcat
    14881 ctacatctct atctatcccc atatgaataa atagattatg tattatagct tgtctttgcc
    14941 ctatatcatt tactattcca aatgcaccta aatctcttgg tataacttct atggccagac
    15001 attcatttct atacttttct cttgtacttt ctagacctat gttagttatt attacaccat
    15061 ttaccattaa aataggtcct tcaaaactta tttttgcagg aactatatct gctatatctg
    15121 ctatactgtt ccttcttaaa aatcttctaa aaatcaaacc aacaatagct cctgacacaa
    15181 tagctaggat tgtgcaataa aaaaagctaa ggccatactt tcttgcaaaa acgatataaa
    15241 ctatcgaaga taccagtgca gaaaaaagac ttatataact tctagattca tacgtagatg
    15301 ctatttcttc aacgtaagcg tctccttttg gtactaattc ttcattatcg atattcttta
    15361 gtgttattct ttcttgttct gcaagacctt gaaactgttg tattgctact gcaaaaaatg
    15421 tcaaggctgc aaactcctta tctataagtg caggtaatgc tatagcacct aacgaagctg
    15481 aaagcccaga tataattatc tgttctaaat aatcttgtgg tcttgatggg tattgtttgt
    15541 ctgtaactct taaaactaat cctctacata atattcctac aactaatgca actataaaag
    15601 agtgtctaaa taaatttgca ttcaataaac tttcttccat tttctcagct ccttctctta
    15661 tatagtttgt gcaaaaaaca aaaaaaattc ccttctattt aaggaaattt ttttgtaata
    15721 ttgtaactat aaattcgcta ccaatgccaa gttcactttt tacttttata catccattta
    15781 gtgatttgac tatatgcttt gttatagcca atcctaaacc cgttcctcct acatccctac
    15841 ttcttgcttt atctacccta taaaatcgtt caaatatgcg ttctacatct tcttttggta
    15901 tccctacacc attgtctata actttaatta cgattttttc ttcatctttt aacactacaa
    15961 cttgtacaat cccattatct tgggtatact ttatagcatt atcaactagg tttaagaaca
    16021 cttgctttat atagtcttta ttggatagta tacttattga tttatcatca catttatata
    16081 aaacttctat atttttttca ctagctgaat gttgagttat ttcatatacc tctttaaata
    16141 catcatatac tacaacattt tccataagaa gagtatcttt attttctaca aatgataaca
    16201 gcaatatatc atcaatcaac ctttttaatc tatctgattc gctttctatt atttctaaga
    16261 atctatttct agtattcaca tctatatttt catttgactt tagtgtttca acaaacccac
    16321 ttatagatgt aagtggtgtt ttaagttcat gacttacatt tgcaacaaaa tcacttctca
    16381 tattttcaag tttaactttt ttagtaatat cttctatatt tactatcgaa cctattatta
    16441 cgttgttagt attctgaaga taaactgggt ctacttttat cttatatacg atatcatctt
    16501 tagtagtaat attagcagtt tcattatctt tagaaccaat aaattgaagt atttgtttta
    16561 atatttcttc ttcttttatt acaacattaa tattatgacc ttcaactaat tgatttggtt
    16621 cactctttat catttctttt gcttcatcat ttatcagaag tatatttcca tttacatcta
    16681 tagccaaaat tccatgagat atactcttta atatagacct taactgtaaa tgtttatact
    16741 gtacttcttc taaggtttta tccatattct ttatcatatg attaaaattt ctagctaatt
    16801 cgccaagttc tcctttggct gatatgttaa gcctagcatg atattcatta ttacttactt
    16861 tttttgagac gtatataaac tcttttaaat atttccttaa tgttattgtg tatcttacag
    16921 ctaatataac aatggctatg gacaacataa caataaaaaa atatatacta tttaaatctt
    16981 ccacaagata acactcctaa aatataacct ttttaatcta ttttgtatcc tacacctctt
    17041 atagtctcta tatatttttc cgatttatct tcatcttcta ttttttttct taaatatctt
    17101 atatgaacat ctactgttct agtttctcca tagtattcat acccccatat tttatccaaa
    17161 agataatttc ttgaaagtac ttttccttta ttttgaatta ataattttaa caattcaaat
    17221 tccttaagag ttaactctat cttttcagag cctttggtaa cttcatgttt tgataaatct
    17281 agttttaaat tacctgtagt aagtatattg ttttcttcac ctaagctagt caaattatat
    17341 ctccttaaaa cagaactaat cctagctaac aactctttta tactaaatgg cttagttata
    17401 tagtcatcag cacctatttc aagcccttca accttgtcat tttccatatt tttagcagta
    17461 agcattataa caggaatatt ttttaagtcc ttatcacttc ttatttttct caaaacttct
    17521 attccactta tatttggtaa catccaatct aaaagtatta aatctggttt aatttcctta
    17581 gcctttataa atccgtcaaa tccatcatat gaataactaa cttcgtaatt tgacacctcc
    17641 aaattgaatt ttaatagctc aactatatgc atttcatcgt ctataacaag cactttagta
    17701 ttcattaaaa agctcctctc tattttatct gtagtaccgt tcctattcgt tctgatgcct
    17761 tattatgttt tctataagag tggaatttat ctgctctaca actagtgcat aaatttaaat
    17821 ttattatatt ttctttttta actccactac atctaagcat atactcatta attttccata
    17881 aatctaaata gtaacttccc tcttttatta tatagaactt ttcatcatta tttgtaagaa
    17941 ttgtgttaaa tttttctact aaatcttcag acacttcata acaacatggt ccaattgatg
    18001 gcccaattat acatactaaa tcttcaggaa ctgttttata gcattcagac attaatttta
    18061 ttgttttatg acctatatta ctaaaagtcc ctctccaacc tgcatgagcc agtcctatgg
    18121 ctttattttt agcatctata attgatatag gtacacagtc tgctgtaaac agtaaaagtg
    18181 gtactttttc caagtttgtt ataagtgcat ctccatctct tcttttccct atagtatctt
    18241 tattgactat attaactata tctgaatgta tttgagaatt actagttaga ttttcaaatg
    18301 tcaaatcaca ttctttacat acttttaaca tatcttctct attttttgca tctatattct
    18361 tacatgttat aactagattt acagaattta atttttcatc aagttcttca caattaccac
    18421 ttaaattaac atactcttta atatcttgta cattttttat atttatataa tctttcatca
    18481 tttgtctcct ttttctatca gtatactttt aagttcattt acaaaagtat ttatatcttt
    18541 aaattgtcta tacacagaag caaatcttac ataggcaacc tcatctaact ctttcaattt
    18601 atccattacc atctcaccga tttttttggt ttcaatttct tctataaaac atttatttat
    18661 ttcattttca atatgtacaa caatactttc aatctcgtca atagaaacag gtcttttttc
    18721 acatgctttt agcaatccat attttatctt ttctctatca aaatattctc tactattatc
    18781 ctttttaatg accattaaag gagttgtttc tattttctca tacgtagtaa acctcttttt
    18841 acatgattca cactcacgtc ttcttctgat agattttaaa tctgtatgtc tagaatctat
    18901 tacctttgat tctttataat tacaataagg acattgcatc tatattcctc ctttgtttaa
    18961 ctatatctca aactcatcta agcttaattc tgaacctata tttactatta ttgtatcaca
    19021 acctatttta agtatatcat tccaaaatat aacttcttca tcccctcctc ttgaaaaaaa
    19081 acttctactt ccatctccaa aaatggtaaa tgagactacc ttaccttcgt gaatatccat
    19141 gtctatgtct attataaacc ccatcctttt tccatttttt acatttataa tttctttttc
    19201 cattatatca gaaactctaa tcatattgta ctcacccttt ctttaaaaat aaatatgtat
    19261 cgaagtaaaa aaaaatgacc tatataggtc atttaaatta aaataatgtt tacagtccat
    19321 ggattactta tacatatttt ctcatatttt ttaaagcatt tttttcaatt cttgatactt
    19381 gtgcttgaga tataccgatt tcatcagcaa cttctatctg agttcttcct ttgtaaaacc
    19441 ttaaatctaa taccaacttt tctctactat ttaatttctt aatagcttct ttaagtgata
    19501 tttcttgcaa ccaattttca tcagtatctt ttttatcttg aacctgatcc ataacgaata
    19561 ttgcatctcc attgtcttga tatactgggt caaacagaga tattgggtct tgtatggcat
    19621 caagtgccat tacaactgat tctacttcta attctaattc ctttgctatt tctgatactg
    19681 taggttcttt agaatttgtt ctaattaatc tttctctaac ttgtagtgcc ttatatgcta
    19741 tatcttttaa tgacctactt actctaattg ggttattgtc tcttagatac cttctaattt
    19801 ctcctataat cataggaaca gcataagttg aaaaccttac attttggctt aaatcaaagt
    19861 tatctatagc ttttataaga ccaatacaac ctatttgaaa taaatcgtct atattttctc
    19921 ctctgttgtt aaactttttt attacactca agactaatct taaattccct cttacaaact
    19981 gttgtctggc ttcttcatca ccatttttta tttgtaataa aagttccttc atttgcttat
    20041 ttttaagaac tggaagttct gatgtattaa caccacatat ttcaacctta ttaacttgca
    20101 tatattttca gtcctctctc aaaagtattc atctattgaa attatttaca tttccaattt
    20161 ttttatactt tttaagaaga attaatttag cattatacaa attttttcat ttctttttgc
    20221 aaccttgaga ttattttctt ttcaagtcta gatatgtagg actgtgatat tccaagcatt
    20281 ccagcaactt ctttttgtgt tttttcaata cctttatcaa taagaccaaa tcttaattcc
    20341 ataatttgct tttctctgtc acttaatctg tcaagtgcca taaccaacaa atctctatct
    20401 atttcttctt caattatttt gtatatttca tcattttctg tacccaatac atcagataat
    20461 aaaagttcgt ttccatctaa atctatattt aaaggttcat caaatgatac ttctgttttc
    20521 tttttattat tttttcttaa gtacattaat atttcatttt ctatacatct cgaagcataa
    20581 gttgccaatt ttatattttt attcagttta aaagtattaa ctgcttttat caaacctata
    20641 gttcctattg aaatcaaatc ttctacatct atacctgtat tttcaaattt tctcgatatg
    20701 tagactacta atctaagatt tctttctatt aaaattgact ttacactttc atctgtttct
    20761 agcttttgta aaagctccat ttcttcttct ggtttcaatg gaggaggaag tatatttgcc
    20821 cctcccatat agtatattcc ctttggcttt attattttta atttaatacc taacatagta
    20881 ataaagctta ttattctttc tttcaatcgt aacattcctt gtccccctta aattttcacc
    20941 taaaatccag gcaatatatt tggattcatt attgctccat attctctatc attaaaattt
    21001 gaaataccaa gtattatatt tcctatcttt tgctcatcta cttctatata gtcagctttt
    21061 aagcctatta ttatgcttgt cttattactt ccagcatgtt tataaggtat tattctaact
    21121 ctacctgaag ttttatcatc taaacttcct ataatttctt gtgctttctt catatctata
    21181 tcttcataat taatatttaa tagtttttct ggaataaatc cttctaacac actagactta
    21241 actattatta catcactttg acttattggg tcttttaaca aattaccact atctactaaa
    21301 gccttacagc aaaaactttt ttctagtaag tttatattta tagtctttgt aagctcctta
    21361 atgtacttaa gcattttcaa atctttataa atgtatttta ataattcaca actcacataa
    21421 gtacatgcaa ttaagaaaga tatttttaag tgacttattc ctgtaaaata aattataaaa
    21481 taagtgcttc cagaaataaa aatgtttact agataaaaaa ctaacgctac atgaacataa
    21541 tcttttttgc tcttacttct aaatgaaatt aaagttatta tactcatgat tattatttta
    21601 aatggtacag taaatagtat attcattgat ggatacaaat aagcaacaga ataggcacct
    21661 cctaaactag cccccaataa cttatttttt ttaggattat atcttttagt aagaatagaa
    21721 gtacaactta taataatata atttattact aggttttcta taacatagta ctcaatatac
    21781 accatgtatc cccccttaaa acctttaaac atattataag atagtatgta caaaatttta
    21841 tgtcactttt gatggtcgaa tccaaaacaa attctattta aaaagaacaa tgatatatgt
    21901 cttatacttt atgtcgtcaa aagatatttt tttaatacca aacaaataga cttttttgta
    21961 gaatccaata tatctaggta gaagttattc actctacttt tggtatattt acaaaaaaag
    22021 actacataat ttaataatat atcaagtaaa tcttttactt atataaaatc aaattatata
    22081 gtctgtaaat tcaaatataa attattgtgc tagtaatcca tttgcatatt ttgcagattc
    22141 atttagtgct tgttctggag ttatgtctcc agctatcatc atttgaagtt gtttccataa
    22201 atattcatat atctctactc catgaggtcc aactgtcaat ggtctataca tatgtttatc
    22261 tcttgtcacc atttcttcaa atcttgggtc tccttggtat ttttcacctt tagcaactgg
    22321 agctctaggt atttctttgt ggaaagcttc cattgattct ttgcttagca tatactcaag
    22381 aagtttaaat ccaagttctt tatttttaca tgctgacatt aatgttaaat catctactga
    22441 agcaaaactt ccaactgtct tatcttctaa tgatgttaca tatccccagt ttaaatccgg
    22501 gaatgattta tcaaaagtac ttgtagcagc agaggatagc attatagtaa atgcagcttt
    22561 tcctggtcca aatacacttt caatcatttc attactatct tttgataaag catcttctgg
    22621 aatatacttt ttcatatcat ttataaagtt tgcagctttc aagccttctg cattattaaa
    22681 tttaactgat tttaaatcat cattatatat atctccacca gcttgccata aatacggata
    22741 ccagttccag tttaagcttc caaatacttt agaaccccaa ccttgtgcaa gaccccactg
    22801 gtcgattttt ccatctccat cagtatcttt tgttgctttt tcacagattc ttttaaaatc
    22861 atcccatgtt tttggaggtt tttctcctaa tttttctagt atatctttat tgtagtacaa
    22921 tacgcctgga ttagcagctt ctataggtaa accataaata ccacccatca tttttgcatc
    22981 atctaagtat agataattat ctgaagttcc tgatttttct aaataaggtg ttaaatcttc
    23041 taccgcaccc atttctataa attgaggaaa catctctgca tacatgtatc ctatatctgg
    23101 accttcacct gcacttattg ctgttgcata tttttctgcg tagttatccc atggtactat
    23161 ctgaaattca atttcacagt tattttcttt ttcaaatttt tcaaatatcg gcttccatgc
    23221 atctgcatca tggtctgtta atggtgcaga ccatacaact aatttttctt taccctcttt
    23281 gcctttagaa tcttcttttt ttgaacatcc tgcaagaatt gatactgata gaatccctac
    23341 cataactaaa gaaagaacct tttttagttt aatcatgctt aatccccttt cagactttaa
    23401 tttgtctttt tcctcaaatg tttacattta attcagattt aatattaaca aataagatta
    23461 ctcttgtcta atcataaaaa actatactaa ctcctataac tatatttttt ttctatacct
    23521 attagattta attcatacta aaaacttact ttttaaattt tatcaatatt taaaaatttt
    23581 tttaagtcta gccacaaact aactatctaa attgagcaaa ataaaaagaa gtctattgac
    23641 ttctttataa gcttacatat tatcttcttc ttcttaggaa tgtaggtatc tccatgctct
    23701 catcatcatc ttcttcaatt atacttgcct ttttaggagg ctctattttt tcctcagcaa
    23761 ttacttcttc ttcaatttct tttacagctt gttttactgt tgtatttaat gaagaacgta
    23821 tgcttgattt aggtttagta tctaaatcta aggcaccatt ttgtaatcct tcaaatcctg
    23881 tcgcgattac tgtaatcatt atttcatcac ctaaatcttc ttttatagaa gctccaaata
    23941 taacatttgc ttctgggtca caggattcca taactaaagt tgatgcctca ttaacttcaa
    24001 ataatcctaa gttaggacca cctgtaacat taagaagtac tcctttagct ccttgtattg
    24061 atgtttctag aagtggagat tgaatagctt gtcttgcagc atcaattgct ctagtttcac
    24121 cactagcact acctataccc atatgagcaa gtcccttgtc tttcattata gtagtaacat
    24181 ctgcaaagtc caagtttatt aacccttcaa ctgctataag gtctgaaatt gattgtatac
    24241 cttgttttaa tacatcatct gcaactgcaa aagcatctaa cattgatgta tttttttgta
    24301 ctatctgtaa aagtctatca tttggtatcg ttataagagt atctactttt gatttaagtt
    24361 ctgctattcc accttcagcg ttcttcattc ttatcttacc ttcaaatgca aaaggtttgg
    24421 taactacacc tactgtaagt atccccattt cttttgccaa gcctgcaacc actggagcag
    24481 ctcctgtacc agtaccaccg cccattccgg ctgttacaaa gaccatatca gctccttgaa
    24541 gtaactttac tatttcatct ttactctcct cagcagctct tttccctact tctggatttg
    24601 ctcctgctcc aagtcctcta gttaattttt ctcctatttg aactttatat tcagcttttg
    24661 atgtatataa agcttgttta tctgtattta ctgatataaa ttcaacgcct tttagttgag
    24721 cttctaccat tctattgaca gcattgtttc caccgccacc tacacctatt actttaattt
    24781 gtgcacattc ttctaattct acgtcaaagt ttagcatcat aaaccccctt ctctcatgat
    24841 tcaaagttat cgtatgtatt aattatatca ttttcttctt ttattttgtt aacctttaag
    24901 taacatattc cacaaaaacg taaattttcc tttttaaatt tcttacttat attgtcaaat
    24961 attactcttt aagtataaaa taatttacgt atatattaaa tgctctttaa tttttacgtt
    25021 ttttgttcat aatattacta ataatatatc gtctaattat agataagtta ttaaacaatc
    25081 tcataccaaa aactaataca gcaacatagt aaagagaaac tcctaacttt tctcctatat
    25141 aagtcaataa tatagccaat atagcattac ctatgaaacc agtaataaaa attacattat
    25201 catagttatc ttcaaaattg gattttatcg ctccaaaaat agaatccatt attgcaagaa
    25261 tagctactga catatatatg gaataatcca tagaatatgt aaatggaata tagtaaccta
    25321 ctactacacc aaatgctaat cctcctaaaa cccataacat aaatcttcac ctctttattt
    25381 tactttgaaa cacaactaca ttacaatcct ttttattaat tttttttact ttttctttat
    25441 ttttttgtat ttttcgtttt attttgcaaa taaatccact ttatttccat tattttcctg
    25501 aacttttata ttaattccat atatatcctt aagagattta gcatatgatt ctggagagtt
    25561 tatagctgca ctcaataaat ctatatctcc tatagctttt attataaatg gttgtccata
    25621 tgtagtatca tttactgtaa tagtagctcc tgaacatttt atcttacttg ttgctagtac
    25681 tctttctcca tttatagaaa ttgctcttgc tccagccttt tttaaatcat ttaaaaccct
    25741 caaaatatct atatcatgta taatcaaatc gtttggattc tgtccgtctt ttaactctct
    25801 ctcactatcc ctcatagtaa tagttatgcc tggccctgta acagttgaat aaccacttaa
    25861 ttctttaact tcttcaagct ctttttgcat aagtgtttgg atagattcat ttccattctt
    25921 ttttgcttct ttatattcat tcaaagtttt tttgttgttt ttaagagttt cttttaaatt
    25981 ttttatttct ttttttgtat tttctacctc actttcaata ctttcagttt gacctaatgt
    26041 tatgtatact ctatttgggt ctagtgattt tatataagtt gatatgaaaa cgcctaatat
    26101 gaatgctcct aatataacta ttttcttttt catactatta tttcccctct gctttgttta
    26161 tatcactatg accatttatt tctatattgt cttctttggt cacttttact ttggcattat
    26221 aatatgtttc tatttcccag acaataccat actttatatt aagtgccgaa gccaatgtat
    26281 cagaatcacc aattgctttt attataattg gttcttttat gacagtgtca ttcatataaa
    26341 gcttgtcttc ttttaaatac atatatgtat tagaaactat tctctggtcg tttatagata
    26401 tggcacttgc ttgtgctgaa tttaatttat ttatcattga caataataaa tcataattat
    26461 atagtatact atcattttta tcagagtttc ctaagtcttt tgtagaattt atctcaattt
    26521 ttatgcctgt tccttcaaca tctgtatatc cagctaatgt ttcatatttt tctatttctg
    26581 acttaagtat gtcttgttct ccttcattgt ttttgcctct ataagtctct atatctttct
    26641 tcacactatt aacttctgat tgtagattct ctttttcttt ttttaaagat ttaagttctt
    26701 ctgtaagttg ctctcctctt ttaaaagttg tcatccctct attttctata ttaataattt
    26761 taagttgtaa actaacaaga acacccaata caatacttcc tatgattata cttttattta
    26821 taaatctttt tttcaatata tcacttcctt acaattgtct atccttctgg actatacact
    26881 gccaactttc catagttaag atttatagtc cctccattag tatttttatt ttgcaggtca
    26941 atcagaattt tacttaccct agatatatta taatctaagt tatcatcatt agaaagtaag
    27001 atttttatat tacttcttgt aagcatttct atattgcttt ctttttttaa gtttacataa
    27061 tttatttttc tataaattcc ttcttctttt aaataattta aagttttaaa tacattctcc
    27121 ttagttttat aatttttaaa ttttatagat ttattatcat caatgctata atctacatct
    27181 actatcaagt cttttttact ttcattagaa cctcttagtt cttccaataa atttcctttt
    27241 ttatctatat aacaataatt atcttcatct tttaatactg caaatatttc tttttctttt
    27301 aaacttataa ttatcttatt gggaagtttt ctttttattt ccacattttc tatatatgga
    27361 ttttttataa gtttattttt catatcttta aaattgtatg caaaaatatt ttcatttaga
    27421 tttacattta gttctttcat tatatttgat ttggtcaccc tcttatttcc aattacatct
    27481 attttcttta catcaaataa atcacttttt acaattatac atatacaaaa agataacata
    27541 agtataaaaa ctaaaactat catgacatta ttcgtattta actttcttct ttttttcaaa
    27601 tataatcaac ccctttggtt aataaataca cctttagagc ttgtttttcc tacaaaaaaa
    27661 ttataatatt taaagtcaaa tttttgcatt ttattacata acatccaaat aaggtattaa
    27721 attttataaa taaatcaata tttcatcaaa ataaacatag catactacat actatttata
    27781 tatttaaatc tgtatacata caaataaacc cctaatttat aataatttag aaaatctaat
    27841 atattataaa ttaggggtaa atatttactt atcttttctt tattcctatt accttagctt
    27901 gaccttctat acttggagtc gtttcttttt ttatttcttt tacttcctta ataacttttt
    27961 ctttttttgt ttttttagaa gttgactttt gtgttgaatt gtatactttc attatctcat
    28021 catatataag gtctatggct tcaggcttac ctatagtttt acttgcattt gccatatcaa
    28081 ccaaaagttc tctatctcct aaaagtttaa atactgctgt atttaggctc tctggagtta
    28141 gatttttttc taaaatagca attcctgccc cttgtttttc tatactcttg gcattatact
    28201 cttgatgatt ttcagcagta taagctttag gtattattat agatggcttt ccaagagccg
    28261 ttatctcagc taatgaaatt gctcctgcgc tacctataac caaatcactt gctgcaagtg
    28321 catttgccat atcttctaag taaggcacaa ctttttggta tggtttaaga tttatatcgc
    28381 taatacttcc cataaattca tcataatagc ttttgcctgt agcaaatata aaagctatat
    28441 cttcatttac catattcttt attacaagtc tcattgcatc atttattttt cttgaacctc
    28501 cactaccacc atagcaaagt accattcttt tttcgtcact tattgataga ttctttcttg
    28561 caatattttt tcttgataat aaaatttctt ttctaactgg atttcctgta aaaactaatt
    28621 tatcttctgc tgcctctgga aatcttttat gcgaatcttc aaaacttgta agtacctttg
    28681 taactgtttt agataaaatc ttattagtta caccaggaaa agaattttgc tcatgtatta
    28741 tggctggtat tttccccata gatgcattaa ataaaacagg tccactaaca tatccaccag
    28801 ttcctataac tatatctggt ttaaatttct taacaatctt tcttgattgc tccaaacctt
    28861 taaaaagctt aaaaactctt tttacattat cgaaatctat ttttcgctta aaaccttgaa
    28921 ctgttacagt ctttaattca aatccatact taggaactat ttctgactct attccctttt
    28981 ccgtcccaac aaaaattatc tcagcgtctg gatgctcatc tcttattttg tttgcaatag
    29041 ctatagctgg atacacgtgc cctccagttc caccgcctga taataaaact ttcatcaaat
    29101 catcatctcc tgttaattaa ttttgacatg ctttgagatg tttaacacta ttcctacagc
    29161 tcccataaag atggtgagtg aagttcctcc ataacttata aatggtagtg ctacacctgt
    29221 agctggcatt gatgatgtcg caactgctat atttaaagct gcttgtattc caatttgtgc
    29281 acctatacct ataacaacca tacaagcaaa tacattagaa gtcttcaaag ctattcttac
    29341 acatctgtag actaaaacta caaatagcat aataactatt atacaaccaa tcaatcctaa
    29401 ctcttctcct attatagcaa atataaagtc attttgtggc tctggtatat aaaaatactt
    29461 ttgtttactc tttcctaagc ctagaccaaa caatccacct gaacctagtg catatagacc
    29521 ttgtattacc tgatacccct taccaagagg gtcttgaaaa gggtctaaaa atgatgtaac
    29581 acgactaagt ctatagggtt cagcaattac taatgcagca aataaagcag ccccactccc
    29641 tatcatagca aatacaattt tcatatccat tccagcaaca aatatcatta caaaagtaac
    29701 tagaattaca gtacctgctg ttgacaaatt tggctgaagt attatcaacg caaaaaatat
    29761 ccctggaact acgagaagtg gtacaacacc ttttgtcaaa gattttattt tatcataatt
    29821 tttttcaatt aattttgcag ttaatattat tgtagcaaac tttgcgattt ctgcaggttg
    29881 aaatgttaaa gctcctattc ccaaccatct ttttgctccg tttgcttcta tccctaaagg
    29941 ggtcaaaact aacaataata atactactgc tatagctcca attgctgtag catttttctt
    30001 ccaaaaacta taatctattc ttgatgtaat aatcataact ataaatccaa gtacagcata
    30061 tattacattc ttttttagaa aatagtacgc atcattgtgt ttaaatgaag attgtataaa
    30121 acttgcacta aacaccataa ctattcctac aaatactaaa agcatagtag tgtaaaacac
    30181 tactccatca aattcagtct tcacccttat gtcaatttgt tttttcaaat tttctttagg
    30241 catccaacta atctacctcc attcttccat ttaagtaagt ttagaggtaa gagattattt
    30301 taaattgttt acattatctt tgaagtcttt tcctcttact tcaaaacttt catacatatc
    30361 ccaacttgca caagctggtg aaagaagcac tatatctcca ctttttgaaa tttcataaga
    30421 agtcttaact gcttcttcca tatcttttac tatatgtatg tcattaaatc ctttattttt
    30481 agcacaattt tctatatttg atgcagtttc tcctaataag accaatgatt taacatatga
    30541 ctttgcagtt tcaagaagtt catcaaaatt acttccctta tccatccccc ctgctataag
    30601 tattataggt ctatcataag actgtatagc ttttatagtg gaatctggat tagttccctt
    30661 tgaatcattt acatatatta cattgtctaa atttcttaca aactcttgtc tgtgttccac
    30721 tcctttaaat gtcataagta catcctttat aacttctaaa tcaattttgc agacatatgc
    30781 tattgctata gctgccatac aattttctaa gttatgacct ccaggtaaac ttacatcatc
    30841 tttatttaga aatttgattt tatcatctat atctattatt atgtcattat tttctaaata
    30901 tactcctcca tttacttttt tagttcttga aaagtaaatt actttggcat tgcatttatc
    30961 agctaaagat tttactatct catcatcata gtttaataca cagaaatcct caacagtttg
    31021 gttcataaat atatttgcct ttgcttctat gtatttttcc atagtatggt gtctatttaa
    31081 atggtcctct gtaatattta atattgcgct tactttaggt ctaaattcat ctatactctc
    31141 aagctgaaaa ctgcttagtt cagttactaa cacagactct tcacttgaag tttctatagt
    31201 atctattaca ggatttccta tattaccaac tacatatgta tctcgttttg ctcttgaaaa
    31261 aatttctcct accaaactag ttgtagttgt ctttccattt gtacctgtaa tccctataaa
    31321 aattggatta tccgatagtt taaaagctaa ttctacctca ccaattatat acttgcctga
    31381 attttttaat ttcaatatga atggtaggtc tagtggtaca cctggagaca ctacaaccat
    31441 atctatatca tctacatctt ctgggtgatg tcctaatatg tattttatgt catttataga
    31501 tttcaattca tcaagtatat ttctcaattt attttcatct tttatatcat ttacgattat
    31561 acttgcaccc aatttatcta aatgtttaat tgtagatata ccagtttttg caagtcctac
    31621 taataaaact ttttttcctt ttaagtccat ttttcattcc ccctagccct aaaatactgc
    31681 tattatactt atccaagcta aaacaactgt tataatccaa aaaataaata caactcttgt
    31741 ttctggccaa ccacactgtt caaagtggtg gtgtattgga gccatcttaa agaatctctt
    31801 tcttgttgcc ttaaaatatc caacttgaat caaaactgat agagcctccg caaaataaat
    31861 tcctccaatt ataggtatta taagcactga gttagttaga actgaaaatg ctacaactgc
    31921 acctcctaat gccattgaac ctgtgtcgcc cataaacact ctagccggat atgaattaaa
    31981 tcctaagaaa cctaaacatg ctccaacagt tgctgctgct aaaactgcga cttctgtatt
    32041 tccagcaata gaacttgcaa atagcataaa gaatacagaa actattaatg ttactcctga
    32101 tgctaatcca tctaatccat cagtcaagtt tacagcattt actattgcta cgattataaa
    32161 catcattatt ggtatatata atattccaac atttatcaca aaatctgtaa atggtatcat
    32221 aagttgtgaa gcacttgatg atgatgtata ctgataaaaa gcaacataaa aagataatgc
    32281 aacttgaagg attatttttt gatatgcttt tagtccaagt gatcttttta gctttatttt
    32341 tataaaatca tctaaaaagc caataaaacc aaaacctgca atacatataa gacctactgc
    32401 catatcatga ctaactttta ctcttgtaag tccagttatt agaattgcaa caatcattat
    32461 aattccaccc atagtaggtg ttccgttttt agctagatgt gtttggggtc catcatctct
    32521 aactgtctga ccaaatttaa attttctaag cattggtata aatattggtc ctattataat
    32581 tactattaaa aacgcaatca atgcggtgta cgtaagctct gttattccta acatcataaa
    32641 actcctctcc cttgtcaatt aactcattag tttaagctta tttatttaga tattcgacta
    32701 ttttttctaa tctcatgcct cttgagcctt ttactaaaat agtgtctcct gtttttacta
    32761 attcatttaa cttgttaaac acatcttctc tattctcaaa gtgatatata ttggctgaat
    32821 tgaatcctaa ttgttttgct tcttctccaa taaacactga attttcacct atagttatta
    32881 taatatcagt attattcata gatgattttc caactaatct atgaccatac tctgaaattt
    32941 cacccatttc tagtatatct ccaagtatcg ctacccttct acctttatat cttccaagta
    33001 tctttaaagc agcatccata gaatctggac tagcattata tgaatcatta attattgtta
    33061 atttttcatt ttttattatg tctagtctca tttttgtccc tttaaaattt tttaaacctt
    33121 tttttatata atctaaagaa atatttagac acaaaccaac taatatagca gacattgcat
    33181 tatatatatt atgttctcct attgttggta taaatatttc ctcttttttc ccatctatta
    33241 cacatataaa tgttaaacta tcctcttcca tagtataact ttcacaataa acatcattgt
    33301 ttttattaaa gccaaatgtt tttaacttat atacatgttc tttttctttt aatgtagata
    33361 aaaacttatc atctccattt acaattaaag tatttgtttc atcaaagttt gaagtaatct
    33421 ccatctttgc tttcaaaata ccctcttgtg aacctagttt ttctacatgt gataatccta
    33481 tattcgatat gactgcaatt ttaggattta ctatatccac taaatatttt atttcattga
    33541 atccagacat acccatctct attacagcac actcatgttc tttatttaga ttaaataaag
    33601 tcaatggaac tccaaaatga ttatttaagt ttttttcatt ttttagtata tttaactttg
    33661 cagatatggc agagtaaatc atatctctag tagtagtttt tcccacacta cctgtaacac
    33721 ctataaatgg aatatcaaat ttttctttat agtattttga aatatctcct agagccaaag
    33781 atgtatcttt tacctctatt aaatttatat ccgaactttc taattttatt ccattacttt
    33841 cattttttat aaaagtttta catccactat catatgcaga cttcataaat gtatgcccat
    33901 ctaaattttc tccaactatg gctacaaatg cattttgggc atttgcctcc ctgctatcta
    33961 taactatatc acttacacag tcattataat ctccatatat taacttccca ttagttgcaa
    34021 gtactaactc ttttattgtt aagcactcca ttattaaact cctcctttaa aaccttagct
    34081 atattctctt agcaacaaaa ggcaatgtat accgttatat atacattgcc tacgataact
    34141 cttaacttta taattatata ctatatttct gtttttttga agatgtttat gggtatatgt
    34201 taattatctg ttacttttta gttttcaaaa tatagggtta tacttgagtc ttcattaact
    34261 tttactccag gttttgggaa tatatccttt acctttgtat cttctggaag ttctatatct
    34321 gcatctaagt ctggttttaa cttcgcatcc tccaatgcct ttacagcatc tcctattttt
    34381 agatttctta catctggcac tttaacttgc ttcttttcat actcagcttt ttcttcttct
    34441 ttatatactg gtttaactcc caaatatttt aaagagtcat tcattatttc tttcactatt
    34501 ggtcctgctg tagtactacc aaatgcactt actcctgttg gttcgtcaac aatagcaaga
    34561 actactattt gcgggtcatc acaaggtgct attccaacaa atgaacatat atactttcct
    34621 ggagcatact taccatctat aactttttga gctgtacctg ttttaccacc aagtctataa
    34681 cctggtatat acgctatctt tcctccacct tcagttacaa ctgattctgc aatttctaac
    34741 atttttttag atgtttcttt agatataaca cttcttactt tttttggttt tactgtttca
    34801 gttatatttc ctttattgtc agtataagat tttaccactc ttggttgcat taaatctcca
    34861 ccattagcta ttgatgatat agctgttata agttgtattg gagttactga tatagattga
    34921 ccaaatgata ttgtagccaa ttcaactggt cctacatttt tttcgttata cagtattccc
    34981 ttagcttctc ctggtaaatc tatacctgtc ttatccataa gaccaaatga ttctatataa
    35041 tcatacattt ttccaacacc taatctgctt cctaactcaa caaatactgg gttgcaagag
    35101 ttttgtactg cttgtttaaa ttcttgtgtt ccatgaggtc tgtaatgtct ccaacattta
    35161 atctttcttc ctccaacagt tacactacct gtacatgtga atttttctcc atctttaatt
    35221 actccttctt caagagcact ggaagatgta ataagcttaa atgtagaacc tggttcatat
    35281 gtatcactta ctgctggatt tctccacatt tgataatagc cttttatttt atctttatca
    35341 ttatattttt caagttcttc ttgataatat ggatatattg gcgtccttga gtcattaggg
    35401 tcataatctg gttttgatgc taaagcaagt atatcacctg ttttagggtt catagctatt
    35461 gcagtaacct tttttgcatt atttaactca taagctttct gtactgcttt ttcagtatag
    35521 tgttgaatta cttcatctat agacaacaca agaccattac cttgtacagg ctcataatat
    35581 ttttccattc cttgtggtat ttctcttcct gaagcatcag tacttactat aagttttcca
    35641 ggctttcctt ttaacttttt atcatattgc atttctacac cagatatacc tgttgcatct
    35701 gaagaagtat gtcctaacac atatggtgca aaattcccat atgggtaata tctttgatta
    35761 tcttctgcca cccatattcc acttaattta gcatctctta tttgacttgc cttatcatca
    35821 tctatccatc tttttacttt tacaagagcc atattttttt tgcttattaa agcgtaaata
    35881 tctttataat cttcatccaa aatctcagct accttttcgg ctgcttcttt tttatcttct
    35941 acttccacag gtttacacca aacagtatac ttagttacac ttactgctaa ctctttcata
    36001 tttctatcat atatagttcc tcttttgggt tctattggta tatctcttgt ttgttgttct
    36061 agtgcttttg tactcaacca atttcccttc ataagttgta gatacccagt cctaataaca
    36121 aggcaaaaaa acaatgcaca tgctagaata agaacgagta ccagcctttt cttacttatc
    36181 ctctttactt ttctcaaatc tattccccct agttatccac atctatatac tttatttgtc
    36241 catcttttgg atagtccata ttgagcaatt ccttagcctt tagctcaatt tcttttattg
    36301 atgttttttc agtttctttt gcctttagtt cctctaatac tatctctttt tttcttaaat
    36361 ctttttgtaa ataatagata tcgtacttca tttccgagat ttttgaataa ccacatagat
    36421 tcaatattat tactgaaaaa gttgcaatca gtaaaatgca tttagctcct atttttttct
    36481 tatttctctt tttataatta ttcttttttt tatttttata ttcatttaga ttatgtattt
    36541 tataattttt tttcaaattc tcaccccgat agtatatagt ttaaattttc ctctatacta
    36601 tatttgttat taatcatatt tcttcctcta aaatttcatc gctaaattaa tacactatta
    36661 cacaatctaa ttgtattttt ttctatacac aacaaaaacc tataaattca cttatacttt
    36721 tatatgaatc tataggttag atattaattt ctatttattt ttatcacttc tagtattttt
    36781 agataatttc ttccataaat ataatatgta actaactatc tactctctga ttcctaagtt
    36841 ctccattata ttacttaata ccatctatat ctatattttt atatctaaat tttcattata
    36901 tatctcaata acaaagaaca tttcttatag tgttaattgt tttttaattg ctttaccagt
    36961 gcaacaaaat atataatact ttttaattaa ttataataat cttatatata agattattct
    37021 ttagccttat aactacttat attttttctg ctactcttag cttagcactt cttgaacgtg
    37081 gatttacttc tatttcttcc tcactaggta atataggttt tctagttatt attttcacct
    37141 cagattcttt atcacattgg catataggta aatgctgtgg acatatacaa tctgatgcta
    37201 aatgcttaaa tgcatttttc actattctat cttctaatga atggaatgtt attatacata
    37261 ttcttccacc ttcattcatt atagaagaag catcatttat catctttgta attactccaa
    37321 gttcattatt tacttctatt cgtattgcct gaaaagttct tttagctggg tgaggtccat
    37381 ctattctggc tttcttaggg atagcttttt taattacatc tactaattct cctgtagttt
    37441 ctattggttt gtttgctctt tcttcaacta taaatttagc tatacgctta gcccagttat
    37501 cttctccata atcttttatt atcttagtta attcatcttc tgtataagta tttacaacat
    37561 catatgcaga aaactcacac ctaacatcca ttctcatatc aagtggtgca tcttgcatat
    37621 aagaaaatcc tctatcagct tcatcaagtt gatgagatga aactcctaaa tcagctaata
    37681 caccatcaat tttgtaaact cctatttttt ctaattcctc ttttatattt tcaaaattgc
    37741 tgtgaacaaa cttcactcta ctttcatatt cactcaatct ctcttttgct gtgcttatag
    37801 catttttatc ttggtcaaac cctataaata atcccttatc tgacagtttt tttactattt
    37861 ctttagaatg tcctgctcct cccatagtac agtcaacata cacgccatct ggctttatat
    37921 ttaaattctc tatacactca tttaaaagaa ctgatacatg atgaaattcc atagttacct
    37981 cccttaaatt cactttttaa actattcttc actaatatta tttttatttt tctttgataa
    38041 ataaacatgt gcaattatgc caccaataac tccaattaga cttataactt gtgccattct
    38101 aagtggtcct aacattaaac tatctgttct taatccctct ataaaaaatc ttcctattga
    38161 atataaagtt atataagtaa ctataacttg accttcatat tttttatttt ttctaaatag
    38221 taataaaact ataaatattc caaaatccca tatagactca tataaaaaag ttggatgtac
    38281 ctttactcca tcaaccatta ttccccaagg taaattggta gcacctccat gtgcttctcc
    38341 attaataaaa tttccccatc ttccaatagc ttgtgctaaa ggcattccaa gtataactgt
    38401 atcagccatt tttaagaagt taatattttt aatcttagtg tatatataac cagctaatat
    38461 tccaccaatt aaccctccat gtatagcaag tccaccacct ctaaaattaa aaatttgaga
    38521 catattttga gcatagtaac tccaattaaa tacaacataa tagattcttg cacagattag
    38581 tccaactgga attgctatta tagctatatt taatacatca tcttctttaa ttcccactct
    38641 ttttgcttct tttattgcta ttaaagttcc taaaatcatt ccacatgcca taagtattcc
    38701 ataccacatg atgtctatgc caaacagtgt aaatgctact ctatccataa cgtcacctct
    38761 ttgtattaat tgaaaatatt tccaaagcaa tatgctataa ttacaattaa ttgtaattat
    38821 actaaattat gataacacat taatgttaat tctacaaaaa atattaaaat ttattctaca
    38881 actccctaca ctatattata tctaattact ataaaatttt ttaaactaaa tctctcatga
    38941 ctaaagttac aagtgtacat agatattttt taatcaaaaa gattgatgca tatatacatc
    39001 aacctcttct aattcttatc tatagctctt aaatttttac ctcaatttat ataaatatat
    39061 tttataaatt tgttttacca aaataaacat ttaaaaacat aaactaatac ttaattagtt
    39121 attcttatta tgattaagtt tctatttagg ctctacaata gaaataacac aatactcttc
    39181 tttaaaatgc tcttttaatc tttcttcaac ttcctcaaaa gtaatatctt taataatatc
    39241 taaataatcc aatagattta tatccttaaa aacataagat ataaaactat tacctataaa
    39301 gtttatagaa tcaaagcact taataaatga ccctattttt ttctttttag ttctctcaaa
    39361 ctcttctttt gataatcctt ctttttttga tttttctata taatcaagaa ttatttcttt
    39421 tactttttta ggctctttag aatctccagc tattatgcta aaagcataat ccacttgaga
    39481 cgaaaatcca gctccaaaat tttcatttat taaaccttgc atatacaaat cttcataaag
    39541 cttacttcct tttttaaata acatccctac aagtatatca gttacaatct ctttacgtaa
    39601 aagttctttc ccttttaaac caacattgct atctttaaaa cctatgttga acataggcat
    39661 agatataggg aatttttcta taacctcttt ttcttttaca ctttctggtt cttctggata
    39721 aaatctttct atactcttag agagtctgtc tactttgtaa ttgtttgact ttttagttac
    39781 atctataact ttttctacat ctaagtcacc aacaacaaat aaagccatat ttcctggatt
    39841 atagaaagta ttataacatt tatatagctc ttccttagtt atcttatata tactatcaac
    39901 agtacctgct atatcaattc tagcaggata atttacatac attgccttca agcaattaaa
    39961 gtatacattc caatcagggt catcattgta catttttatc tcttgtgcta ttataccttt
    40021 ttccttttca acattttcat ctgtaaaata aggtgtttga acatagtcaa ttagatgttc
    40081 taaactctca taaaaatttt cagtagcaga aaataagtag gctgtcattg taaaattagt
    40141 aaatgcattt gcatttactc ctaattttga aaatttatca aatgcatctc ctccatcagg
    40201 ttgctcaaac atcttatgct ctagaaaatg tgcaatacct tcattcactc taattttctt
    40261 atcttctcca attggtacaa attctaagtc atttgaacca taattagtag ccaaaatagc
    40321 atactttttc ataaaacccc ttttaggcat aaaatataca tctaatccat tttgcaattt
    40381 ctcataataa acttcttctt tcaatatatc attaactatt ttttccatag acatctcctc
    40441 cattaatttc tcaagaagta tacagtatca agctctatat tttttatagc ttcaactata
    40501 tctttttttg taaccttttc tatactagat attatgtctt gtacttcaga atttgtcttt
    40561 gccatagact gagagaaaac aaaatcagac ataccaccta tattatcagt tatagatttc
    40621 atagaattaa ctaaagcaag tttactgttt tctaattctt catcagatat atcacctgct
    40681 tttacacttt ctacttgttt ttttatcaat tctacagctt tatcataatc tttagtttct
    40741 ataccagatg atataaacat gatactttta tacttctcta ttgaagagaa tatatagtaa
    40801 cataaacttt ctttttctct tacattaaca aataatttag agtgtggacc accacctaag
    40861 acattgctcc caactactaa agcataatat ttatctatgt ctgcataatc cacatttgct
    40921 ctataaccca taactaactt accttgagtt atttccattt gttcatctat aacttttacc
    40981 tcatccacat tttttataaa atctgctctt ggtatattta taatttcttc tctttcaaat
    41041 tttagatttt tacttattat atctacaact ttatcttcat caaaattacc ttctacaact
    41101 atatctatag gtgatgtttt aagtatgttt ttataatgtt catataactc tcttgaagtt
    41161 atactatcta tttcatcttc ataaccaaat tcacttacac tatatctctc tcctttacac
    41221 atagcctcaa tacatttatc ttgcgcatat tcctttttat catttattat tgattgtatc
    41281 ttttctctta aattctcttt ttcaatattt aaatattctt ctttaaaccc accatctaca
    41341 acaagcgtat tatttattac ttcattaaaa aattctacta cttcttcaaa tatactctca
    41401 tctaaatact tttcacttat gttaattact ttaaaactca atacttgacg ttctccacgt
    41461 tttacagcat cagctcccat agatgaacca tataaatcat ctaatttatt ggatattgct
    41521 ctagcactag gatatttagc gcttccactt gctattattg atggtattag agcatttttt
    41581 gtagtttctt tttcatccaa tagtctctga acatataaac ttataagatt cgatttaaac
    41641 ttttctgttt ttatcaaagt aagattaata ttattaccta aatttattat ttttttagta
    41701 ttaaccatat gtacctccta tttttattta aacatcaatt ttaaaattga aatttattta
    41761 ttttttaatt acatatactc acatataaaa accttctatt taattatatc ctttttatta
    41821 ttttctaaaa gcaatatttt tatttaaatt aaatacaaat attatttaaa tcaatatgct
    41881 ttaaagtcta gtttacaagc ttgatatata tgatgtataa tcaatatgta tgtctataat
    41941 taaaacaatt ttgcaatcct atagattgat tgtttaaagt tatataaaga atataaaaaa
    42001 gaataagaga gaggacgatt aatatgaaat taggtatagt tggtttacca aatgtaggta
    42061 aaagcacact atttaacgca ataactcaag caggtgcaga atctgcaaat tacccttttt
    42121 gtacaataga cccaaatgtg ggagttgtat ctgttccaga tgaaagatta aacaaactac
    42181 aagaattata taattctgaa aaaatagttc ctactgctat agaattttgt gatatagcag
    42241 gtttagttag aggagcttct aaaggagaag gtttaggaaa taaattctta tcacatataa
    42301 gagaagttga tgcaatagtt catgttgtta gatgttttga ggatgaaaat gttgtacatg
    42361 tagatggttc tgtagaccca cttcgtgata tagaaactat aaacttagaa ttaattttct
    42421 cagatataga gattcttgaa agaagaatta ataaaactca aaaagctgct aaggctgata
    42481 aaactttagg ttctgaactt gatttattaa aatcaataat gtctacttta gaagaaagta
    42541 aatgtgtaag aactatggaa tttacagaag atgaacaaac attcgtaaat tcattggatt
    42601 tacttacatc taagcctgta atatatgctt caaatgtatc agaagatgat ttagctgata
    42661 atggagaaaa taataaatat gttcaacaag ttaaagcatt tgcagaaaca gaagatgccg
    42721 aagttgttgt agtatgtgca caaatagaag cagaaatttc tgaacttgat tctgctgaag
    42781 aaaagaaaga gtttttagaa acattaggtc ttgaacaatc agggcttgat aagcttataa
    42841 aatcttctta tgctttactt ggtttaattt cattcttaac tgctggtcca aaagaagtta
    42901 gagcatggac tataaaagtt ggaagtaaag ctcctcaggc aggaggaaaa atacattctg
    42961 atatagaaag aggatttatc agagcagaaa caatagcctt taatgatttg gttgagcatg
    43021 gtactatggc tgctgctaaa gaaaaagggt tggttaaatt agaaggtaaa gaatacatag
    43081 ttaaagatgg agatgtaata ttatttagat ttaatgttta aaatgattaa tcgtatacac
    43141 tttgactatt atatataata actaaaaaac t
//
LOCUS       DAYATN010000019        39542 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.019, whole genome shotgun sequence.
ACCESSION   DAYATN010000019 DAYATN010000000
VERSION     DAYATN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.019"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..582
                     /locus_tag="ACGI8A_001014"
     CDS             <1..582
                     /locus_tag="ACGI8A_001014"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HFH0179022.1"
                     /translation="KMFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITT
                     MLFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENIN
                     IDLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITISS
                     LSLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            636..758
                     /locus_tag="ACGI8A_001015"
     CDS             636..758
                     /locus_tag="ACGI8A_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HFH0179023.1"
                     /translation="MKFASSLALSTAILSANSTCPWIIHQPKVPKEISNLKKTN"
     gene            1054..2433
                     /gene="scfB"
                     /locus_tag="ACGI8A_001016"
     CDS             1054..2433
                     /gene="scfB"
                     /locus_tag="ACGI8A_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HFH0179024.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            2462..2959
                     /locus_tag="ACGI8A_001017"
     CDS             2462..2959
                     /locus_tag="ACGI8A_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFH0179025.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            3257..4921
                     /locus_tag="ACGI8A_001018"
     CDS             3257..4921
                     /locus_tag="ACGI8A_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HFH0179026.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEAIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            4934..7384
                     /gene="recJ"
                     /locus_tag="ACGI8A_001019"
     CDS             4934..7384
                     /gene="recJ"
                     /locus_tag="ACGI8A_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFH0179027.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            7397..7909
                     /locus_tag="ACGI8A_001020"
     CDS             7397..7909
                     /locus_tag="ACGI8A_001020"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFH0179028.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            7983..10190
                     /locus_tag="ACGI8A_001021"
     CDS             7983..10190
                     /locus_tag="ACGI8A_001021"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFH0179029.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            10215..10664
                     /gene="dtd"
                     /locus_tag="ACGI8A_001022"
     CDS             10215..10664
                     /gene="dtd"
                     /locus_tag="ACGI8A_001022"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFH0179030.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            10679..11293
                     /locus_tag="ACGI8A_001023"
     CDS             10679..11293
                     /locus_tag="ACGI8A_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0179031.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            11384..12940
                     /locus_tag="ACGI8A_001024"
     CDS             11384..12940
                     /locus_tag="ACGI8A_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HFH0179032.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            12930..14192
                     /gene="hisS"
                     /locus_tag="ACGI8A_001025"
     CDS             12930..14192
                     /gene="hisS"
                     /locus_tag="ACGI8A_001025"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFH0179033.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            14266..16053
                     /gene="aspS"
                     /locus_tag="ACGI8A_001026"
     CDS             14266..16053
                     /gene="aspS"
                     /locus_tag="ACGI8A_001026"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFH0179034.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            16512..17744
                     /locus_tag="ACGI8A_001027"
     CDS             16512..17744
                     /locus_tag="ACGI8A_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFH0179035.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            17758..18564
                     /locus_tag="ACGI8A_001028"
     CDS             17758..18564
                     /locus_tag="ACGI8A_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HFH0179036.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(18676..19545)
                     /locus_tag="ACGI8A_001029"
     CDS             complement(18676..19545)
                     /locus_tag="ACGI8A_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0179037.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            19917..21344
                     /locus_tag="ACGI8A_001030"
     CDS             19917..21344
                     /locus_tag="ACGI8A_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HFH0179038.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVEIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(21421..21690)
                     /locus_tag="ACGI8A_001031"
     CDS             complement(21421..21690)
                     /locus_tag="ACGI8A_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179039.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(21693..23135)
                     /gene="nhaC"
                     /locus_tag="ACGI8A_001032"
     CDS             complement(21693..23135)
                     /gene="nhaC"
                     /locus_tag="ACGI8A_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFH0179040.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(23160..24326)
                     /locus_tag="ACGI8A_001033"
     CDS             complement(23160..24326)
                     /locus_tag="ACGI8A_001033"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFH0179041.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(24551..26671)
                     /locus_tag="ACGI8A_001034"
     CDS             complement(24551..26671)
                     /locus_tag="ACGI8A_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0179042.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQNFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            26904..27305
                     /locus_tag="ACGI8A_001035"
     CDS             26904..27305
                     /locus_tag="ACGI8A_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179043.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            27348..27641
                     /locus_tag="ACGI8A_001036"
     CDS             27348..27641
                     /locus_tag="ACGI8A_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFH0179044.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            27656..28096
                     /locus_tag="ACGI8A_001037"
     CDS             27656..28096
                     /locus_tag="ACGI8A_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HFH0179045.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(28188..28661)
                     /locus_tag="ACGI8A_001038"
     CDS             complement(28188..28661)
                     /locus_tag="ACGI8A_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFH0179046.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(28661..29449)
                     /locus_tag="ACGI8A_001039"
     CDS             complement(28661..29449)
                     /locus_tag="ACGI8A_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFH0179047.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            29777..31021
                     /gene="glyA"
                     /locus_tag="ACGI8A_001040"
     CDS             29777..31021
                     /gene="glyA"
                     /locus_tag="ACGI8A_001040"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFH0179048.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALITSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            31344..32441
                     /locus_tag="ACGI8A_001041"
     CDS             31344..32441
                     /locus_tag="ACGI8A_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0179049.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            32432..33130
                     /locus_tag="ACGI8A_001042"
     CDS             32432..33130
                     /locus_tag="ACGI8A_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0179050.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            33133..34197
                     /locus_tag="ACGI8A_001043"
     CDS             33133..34197
                     /locus_tag="ACGI8A_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFH0179051.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            34380..35084
                     /locus_tag="ACGI8A_001044"
     CDS             34380..35084
                     /locus_tag="ACGI8A_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0179052.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            35077..36399
                     /locus_tag="ACGI8A_001045"
     CDS             35077..36399
                     /locus_tag="ACGI8A_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFH0179053.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            36586..37959
                     /locus_tag="ACGI8A_001046"
     CDS             36586..37959
                     /locus_tag="ACGI8A_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179054.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(38331..39269)
                     /gene="pdaA"
                     /locus_tag="ACGI8A_001047"
     CDS             complement(38331..39269)
                     /gene="pdaA"
                     /locus_tag="ACGI8A_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HFH0179055.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        15117 a         4544 c         6727 g        13154 t
ORIGIN      
        1 aaaatgttta aaagatatgc tgaaaaaatg acatctgttt taatttgtaa taatatgata
       61 gataataatg aatctaaagt ctattcttat ggatttgaaa tattaatagc tttcatagtt
      121 aatataacca ccatgctgtt tataggtttt ttatttggta aatttacata tgtattattt
      181 tttttaatgt gttattgtcc aataagacaa ttttcaggag ggtatcatgc agacaattat
      241 tttagatgct tattaacttt tatttttata attttatcca caatcttaat tatagaaaat
      301 ataaatatag acttatttaa aaatattatt atgattattg caagtgttag ttgggttgga
      361 atttgtgttc tgtgcccaat agaacatagg agtaacccta taagtgatag ggaaaagttg
      421 gtttataaga aaactgctat atttatatct acagtagtgt tattgataac aatttcaagt
      481 ctttctttga gtatatttgt agattatttc acatatagcg catttgccat gttttggatt
      541 tttgtgatgt tagtattagg taagttaaaa gctaaagtat aagtaaaata tgtatgaggg
      601 agagttaagt tatgaaaaag tttattgtaa gatttatgaa gtttgctagc tcattggcac
      661 ttagcactgc tattttgtca gctaatagta cctgtccttg gattattcat cagcctaaag
      721 tacccaaaga aatcagcaat ctaaagaaga caaattaaat acaagcactt atttatacaa
      781 aattatcagg aaaaaagaaa ttattatatt ttaataagat taaaacaata taaaatcaga
      841 tacaaaattt attttatagg agcctaatac ctatttggta atatattagt gcgccaattt
      901 agggggatat agtgtattta atgaaatgac ttacggagag taagtcattt ttgtattgta
      961 tgtattttat gtaaacttat gatataatga agggtaaagt ttaattaaaa taataaaaaa
     1021 ctttagtata gatagtaatt taggaggtag ggtatgagta tgatacataa attttctatg
     1081 aatggatata atatcgtttt agatgtgaat ggtggagctg ttcatgtgtt agatgatgtt
     1141 gcatacgatt tgttagattt ttataaagaa aaaagtaaag aagaaatatt agagatatta
     1201 aaatcaaaat atcaagaaga aaaaataaat gaagcatatg aagaaatatt aaatttggaa
     1261 aaagagggtc ttttatatac agaagataca tatcaatatc atccaagctt tgtacataga
     1321 gagcctgtag taaaagcttt atgtttaaat gtagctcatg attgtaactt aaaatgtaaa
     1381 tactgttttg cagctcaagg tgattttgga ggagaaaaag aacttatgag ttttgaagtt
     1441 ggtaaggcag caattgacta tttgattgct aattcaggaa gtagaaagaa cctagagata
     1501 gatttctttg gtggagagcc tttgatgaac tttgaagttg taaaacaact ggttgactat
     1561 ggaagaagtg tagaaaaaga ttataataaa aatataagat ttacaataac tacaaatggt
     1621 gtactgctaa atgatgagat tatagattat ataaatgaaa atatgcataa tgtagtttta
     1681 agtttagatg gaagaaaaga agttaatgat aatatgagac caactttaaa tgataaagga
     1741 agttatgata ttacattacc acgatttaag aaacttgtgg aaaaaagagc aaaagataag
     1801 tattattata taagaggtac ttttacaaga gataatttag atttttcaaa agatgttatg
     1861 cattttgcag atttaggatt taaattaacc tctgtagagc cagtcgttgg agatgagagt
     1921 aatccatatg cacttagaga agaagattta cctaagatat ttgaagagta cgaaaaattt
     1981 gctgtagagt atgcagatag acaattacaa ggagatggat ttaaattttt ccattttatg
     2041 atagacctaa atcaaggtcc atgtgtaata aagagaataa ctggatgtgg agctggaaat
     2101 gagtatcttt ctgtaactcc aaatggagat atctatccat gtcatcaatt tgttggaaat
     2161 gaagaattta aaatggcaaa tatatttgat gaagaaatag tacttccaga aaatttaaaa
     2221 aatatgttta gagaagctca tgtatatact aaagaagagt gtaaacaatg ttggaataag
     2281 ttttactgtt caggtggatg tcatgcaaat gcgataaact ttaataatga catatcaaaa
     2341 ccatatgaat taggatgtga aatgcaaaga aaacgtacag agtgctctat aatgatacaa
     2401 gctaagttaa tgttagaagg agcaaccaac taaatttata ttttaaagga gttgtttata
     2461 tatgattaga gattatttag aagataaacc tttaattgac gaaagtgtat ttgtggcaaa
     2521 atcagcagat gtaattggaa atgttaaaat aggcaaagat tcaagtatat ggtacaatgc
     2581 agtagttagg ggtgatgaag gaccaataac tattggagaa aacactaata tacaagattg
     2641 ttctatagtg catggagata cagaaactat aattggtaac aatgtaacag ttgggcatag
     2701 gtctatagtt catggttgta aaattagtga taatgtgtta attggtatgg gttcaataat
     2761 attggataat gctgaaatag gagaatatac tctgatagga gcaggtacac taattacatc
     2821 taataagaag tttccgccag gtgttttgat aatgggttct cctggtaagg ttgttagaga
     2881 gcttacagaa gaagataaaa aatatattga tgaatcttat gaatggtatt tagaggcagc
     2941 acaaaatcaa aaatattagt ttaaagagtg gttgtttcaa aacatattta aatctgtttt
     3001 gaggcaacca ctaatttatt ttattagatt ttgttgatat tttagttttc aaaaagtatt
     3061 tgattaaacc agatataaaa tgtcaaaaat aaaaattaac tgagttaaat aattttaata
     3121 aaattggtag tatatgtgat aaaattaaat attaaagagg atataggata ttagaatatc
     3181 aattggtgaa ataatgtcat atacgataaa ttaaggtgta agtttttggg tatacctatc
     3241 ttaggagtga aaagatttga gaataagtta tagaaattta aagcgatata gagaaattgg
     3301 atatgtttta ataaagtatg gttttagttt tattgtagaa agactaaata tagaaggaat
     3361 tgcctataaa ataccactat ttgacccacc agaagaaata aaaaatatga caactggtga
     3421 gagaatgaaa agagttttag aagaactagg acctacatat ataaaaatag gacagatatt
     3481 gagtacaaga aaagacttgt tagaccagga tataattgat gaaatatcaa aactcagaga
     3541 tgatgtagag aaatttgatt caaatatagc tattgatata tttaaagaag aagtgggttt
     3601 aagtatagaa gaaatattct tagaatttaa agaagaagca atagcagcag cttcaattgg
     3661 tcaagtttat gaaggtgtac ttaaaacggg agaagaagta atcgtaaaaa ttcaaagacc
     3721 aaatatagag aaaataataa aatctgattt agagattctt agaactattg caaatacttt
     3781 aaaagattta aaaaaggatt ttaatttaga tttagtacag atgattgaag aatttcaaac
     3841 tcaattaatg agggaacttg attatacatt tgaagctata aatgcaacta aattctctag
     3901 gatatttaaa aatagtgatg aagtatatat acctaaagta tattcagaat ataatactaa
     3961 gaaaatttta gtaatggaga aagttaatgg tacaaagtta agtgatgtag aaaaaataag
     4021 gagactaggt tataacacaa aaactattgt tgaaataggc gttaggtcat tttttacaca
     4081 ggtattatct catggatttt ttcatgcaga cccacatcca ggtaatatat tcgtagttgc
     4141 taaaaataag atagcatata tagattttgg aatgattgga attattgata ataaaacttt
     4201 aaaccaatta aatgaaattg cattagcagg tgtagaaaag aatgttgaca agataatata
     4261 tctattgata gaaatggatg cattaaatgg agaggctgat attaagggac ttagacagga
     4321 tttactttat ctaatacatt attactatga tatttctata gaaaaaataa atgtaacaga
     4381 tatattaaat gaactattta ggttttttag acagtataaa atagtcatgc ctgcacaatt
     4441 tgtaactctt gcaaagacag ttataacatt agaaggaaca agtagaactt taaacacaga
     4501 tttttctttt ggttctatgg gaaaagagtt tatgaaacat cattataaga gtaaatttaa
     4561 ccctaaaaat gttgttttaa gttcaagaca aaatgtagaa gaaatattac tggacataaa
     4621 aactattcca aagcaattaa aagcaatttt aaaaaatata gaaagaaata atataaaaat
     4681 gcaaattgaa gatgttaaaa tgacgaggtt agaaaattgt ataatagagc taacatctca
     4741 aatatctttg agtttagtgt tggcatctat tatagtaggt tcttctctta taattgcatc
     4801 tccaaatata gaaaataata tatggataaa atttactgca atagcgggtt tctttatatc
     4861 ctttataata ggcctttgct tagttataag aagtattagg tcaaaataca aaaaagatta
     4921 gggggaatta aatttgaaaa aatggatatt gaagtataaa ggtaagatag aggaaagtga
     4981 atttagcaaa aaacttaata tttccccgga aatctgccaa attttaaaaa atagagggat
     5041 atgtactgaa aaagattcag agatatttat gaatccatct ttagattatt taagagaccc
     5101 ttttttaatg aaagatatgc aaaaagctgt tgatagaata aagttagcag ttgagaagaa
     5161 cgaaaaaatt tggatttacg gagattatga tgttgatggt gtatcatcta catctatctt
     5221 gtgcttatat tttaagagta taggttacaa tgttgattac tatattccta atagattaga
     5281 agaagggtat ggtataaatg aagatgctat taagcttata agctctaggg gatgtgactt
     5341 gattataagt gttgactgtg gaattacatc agtgtctgag gtcaatacag cgaatgattt
     5401 gggcatagat gttataatta cagaccatca tgaatgtcaa agtgaaattc catcagcctt
     5461 tgcagtaata aatccaaaac aagaagattg taattatcca tttgattcac tatgtggatg
     5521 tggtgtagca tttaaaatga ttcaagcctt gactccaaaa gaagaattta aaactagtat
     5581 gtataattat ttggaaatag taacactagc aactatttgt gatattgttc cacttataga
     5641 tgaaaataga ataattgtaa aaaatggttt aaaatcaatg aaagaaggca aaaatattgg
     5701 attaagggag cttataaaag tttgtggtgt agaaagtgat aagataggtt cttctcatat
     5761 aggttttgca ataggtccta ggataaatgc ttcaggtcga ttgggatatt cttacttagg
     5821 agtagagcta tttactactc aatcacaaga agaggcagta gaaatagcta gtatattgga
     5881 agaaaaaaat aatgaaagac agatgataga agctaagatg tatcatgaag ctgaggaaat
     5941 gctaaagtct aatagcagat acaatgatga caaggtgttg gttttagcta aagagggttg
     6001 gcaacatgga ataataggaa ttgtagcatc aaagttaact gaaaaatact acaaaccaac
     6061 aattcttcta ggtatagaaa atggggaggc tactggttca gcgaggtcaa taaaaggatt
     6121 taatatattt gaagctctca taaagtgcaa agacttgatg actaagtttg gtggtcatga
     6181 acaagcagca gggttatcct tggattcaga caatgtagaa attttagcaa atgaaataaa
     6241 taaatttgca gattataatt taactgaaga tgatatgata gagaatgtaa atgttgagtt
     6301 tgaattacaa gaaaatgtaa taaatttaaa tcttgtagaa gaattacata agttagaacc
     6361 ctttggatta aataatccta atcctagatt tatagttaga aattatattt taaaagattt
     6421 gaaagttata ggtaagaatc aacaacatct gaaattaagc atagaaaaag agaaaagcta
     6481 tgaatgtata ggatttaata tgagtcattt aaaatcaatg tataaagtag gagacaaggt
     6541 agatgtacta ttccaattag atgaaaataa ctatatggga aataggaaag tacaattttt
     6601 acttaaagat atacgattgg ctcgacctaa aagtgcaagt aatgacaaac tttcattgaa
     6661 attaatgtca aaaataatac caaaagatac tcaaagcctg tataatatat ctgtgtctga
     6721 ttttgaatta tttgatggaa atacagatat aaatatattt gattattttg aaaaagatac
     6781 actaataata tctaattcta taaatggatt ttatagagca atgtcagata tatcacttat
     6841 agatttggat tttaacataa attacaatat tattgaagat gttagcaaaa atactgataa
     6901 attagagctt atattttctc ctaatattga taaaatagac ttaaaaagat ataataatat
     6961 tattctttat gattatttat acaataaagg cgaatattcg tatatatatg agaataaaag
     7021 agaagaaagt gaaattataa aatattataa taaaacagac ctattatatt taaagaatgt
     7081 agttagtaat atagttccat caagagatga atttataaca atttataaac aagcactcat
     7141 aaaaaaagaa attgatttgg atatggtaaa tataagggaa acttttaatg taattccatt
     7201 aaagtttttt acgatattaa atgtatttag agaattaaat ttacttgact ttaatttaaa
     7261 ttatgaaaaa aattctgtac tcataagaat attgccaaaa ccacagaaaa aattagactt
     7321 aaatgagagt ttaatactca ataatctaaa aaatttagaa aaacaataca atagcagtta
     7381 ttaggaggcg tttcccatgg atttaaaaaa ctttataagg aatattgatg atttcccaaa
     7441 gccaggcata gattttaaag atgtgacaac tctatttaaa gatggagatg catttaaata
     7501 tgcagtggat tctatagttg aagaattgaa agataaagat gtagatttag taataggacc
     7561 agaagcaaga ggatttttga tgggtacacc tgtagcatat gctttaggag taggatttgt
     7621 tccaattaga aaacctggaa agttaccagg tgaagttgag agttatgagt atggattaga
     7681 gtatggtaca gatactttag agatacataa ggatgcaatt aaaaagggtc aaaaagtagc
     7741 aatagtagat gatttattgg ctactggtgg aactatggaa gccgctgcca agcttgtaga
     7801 aaaattaggt ggagaagtag tttctatgca atttttgatt gagctaaaat tcttaaatgg
     7861 gagagaaaag ttgtctaact atgatgttaa ttctcttata aaatactagt acttttataa
     7921 tagttggtat aacaccaact attttcatat atattaaaat tttatacaaa ataggtggtt
     7981 atatgcatga taaagaatta caagaaataa ttgaaaaaat aaaagtttat gcacctaatg
     8041 caaattttga aatggttgaa aaagcctata atcttgcaaa aaaagcgcat gaaggacaat
     8101 atagaaaatc tggggaacca tactttattc atccattagc agttgcaaat attttaatag
     8161 agatggaatt agatattgaa actataacag caggtttact tcatgatgtt gtagaagata
     8221 cagaatatac ctatgaagac ataaaaaaag aatttggcaa agaagtagcg gatttggttg
     8281 atggagtaac taaacttggt caaataaaat atcgttcgaa agaagaaact caatctgaaa
     8341 atttaagaaa aatgtttttg gcaatggcga aggatataag ggttatactt ataaaacttg
     8401 ctgatagatt gcacaacatg agaacattaa aatacatgcc tccagaaaaa gcaaagtata
     8461 aagcaaaaga gacacttgaa atttatggtg gtatagctca tagattagga atttccaaga
     8521 taaagtggga actagaagac agagcattaa gatttatgga cccagaaggg tattatgatt
     8581 tagttagtag agtatcaatg aagagaagtc aaagagagga ttatattcaa ggaattgttg
     8641 aacttcttaa agataagttt aaagatttaa gtataccatg tgatgtatat ggtagaccaa
     8701 agcattttta cagtatatat agaaagatgc aaaaaaaaca taagactttt gaagagattt
     8761 ttgatttaac agcagtaagg atattggttg ataatgtaaa ggattgctat gcagtacttg
     8821 gtatagttca tacattatgg agacctattc caggaagatt taaagattat atagcaatgc
     8881 caaaacccaa tatgtatcaa tcattacaca ccactgttgt aggtcctgat ggagaacctt
     8941 tagagataca aataagaact catgagatgc ataatatagc tgaaaatggt atagcagccc
     9001 attggaaata taaagaaggt atctctagca aagaatcaaa ggttgaagaa aaacttcaat
     9061 ggttgagaca gatgatggag tgggaaaaag atttaaaaga cccacaagag tttatggatg
     9121 cacttaaaga agatgtattt aatagtcaag tttatgtatt tacgccaaaa ggagacgtaa
     9181 ttgaattacc agcagaatca acacctatag attttgccta tagggttcat acaaatgtag
     9241 gcaataaatg tgtaggagct aaaattgatg gaagaatagt tcctattgat tataagcttc
     9301 aaaatggtaa tattgtcgag gtacttacat catctaattc taatggacca agtagggatt
     9361 ggcttaatat agtgaagacc ccaaatgcaa aaagtagaat tagacaattt ttcaagaagg
     9421 aaagaagaga agaaaatgtt gaccgtggaa atacaatatt agaaagagaa tttaagaagc
     9481 atgatatacc acaaaaagac cctcttgttg aaaaatatat ggcattagtt gctaagaaat
     9541 ttaatcagcc aagtgttgag gacttaattg ccactgtagg ttatggtggt ataatgtcat
     9601 ctcaagtggt atctaaggta aaagacctat atttaaaaga ggttaaaaaa gctaaaaagg
     9661 aacaacagat acaagaagaa gatttaaata aacacaatat aagtgaaaca gaatacagta
     9721 aaaagagaaa gaaaaacgca tcacaaggaa ttatagtaaa agggcttgac aatatcttag
     9781 taagatttgc taagtgttgt aatcctcttc caggagatga aattgtagga tatattacta
     9841 agggtagggg agtagccata cacagagctg attgcccaaa tgcaaacaat aatggtgatt
     9901 tcttccaaaa tagattggtg gaggtttcat ggagttcagc aggaaaaggt aagtttgaag
     9961 cggaaattca aataaaagct attgatagaa gaggaattgt taatgaaata actcatattg
    10021 ttgcaatgga taaacttagt ttaaacggaa taaatgcaag aaaaggtaag gatagtattg
    10081 taagtataaa cttacttgta gaggttaatg acacagagga attaaaaatt ctaatgaaaa
    10141 aattaaaagc aattcctggt gtagaaagta tttatagaat gacaaattag agttaaaata
    10201 atagggagga aactatgaga gcagtagtgc aaagagtatc ttcatctaaa gttacagtag
    10261 atgaaaacac tataggtcaa ataaataagg ggttactagt attgttagga gtgactcatg
    10321 atgataaatc aagtgatgta gattatatga ttgataaaat attgaatctg agaatttttg
    10381 aagatgaaaa tgacaagatg aatttatctc taatggatat aggtggagag cttttagtag
    10441 tatctcaatt tactttatat ggagattgca gaaaaggaag aagacctgga tttagtaatg
    10501 cagctaggcc agaacttgca aataaccttt atgaagaatt tgtaaaaaaa gcaaaagata
    10561 aaggtgtgac agttggtaca ggtcaatttg cagctcatat gatggtagag ttaactaatg
    10621 atggtccagt aacaatacta ttagactcaa gtaaatcttt ttaaggggga aaactttaat
    10681 gattataaaa aaatttgtag atagttattt tggagtaaat acttatgtat taggtgatga
    10741 aaagactaaa aaatgtgcag ttatagaccc aggtggaagt ttagtagaga cattaagttt
    10801 tgtaaaagaa aatgaattaa atattgaata tattatcctt actcatgggc atggagacca
    10861 tataggatat gttaaggata ttaaagaaaa aactggtgct aaagtagttg cacatgttga
    10921 tgaaaaagaa ttattaaatg acaaaaataa aaatctaagt tatacaatga gatgtggagc
    10981 gcaagagttt gatgcagata tctatgtaaa tgataaagac aagcttgaac ttggtgaatt
    11041 aaaaatgact ttcttgcata ctccaggaca tactcaagga tgtatgtgta taagagttga
    11101 agatgaaatg ttcacaggag atactttgtt tgctggaagt attggtagaa ctgatttata
    11161 tagtggagac tttaaccaaa tggaaaaatc acttaagaaa ttatgtaaat atgaagatgc
    11221 tgttagagta tatcctggac atggaccaac cagtactcta ggggttgaaa agaagacaaa
    11281 tccttacatg taattttata ttagtattta aaaaagtata aaatatttaa taactatatt
    11341 agtgaggggg ttcttctact gttaaataga aggagttaag ttgatgttgt atgttttttt
    11401 aaaaggacat gattataagt atgaagtagc agaacttata aaattgttta ctagtgaatt
    11461 taagtttaaa gactctaatg ctaacaataa tatagagtca aagcatttaa taaatagatt
    11521 gatttatgag aatggtgtat tattctcaag tactgaatac tatgaaaatg gaaacttaaa
    11581 gtacgagtca atacagaata tagaaaatat gaatcttgaa tttttagatg gatttgatag
    11641 cagcaaaacg attgaagaat ttcttaatag cctagatagt gaaagtaaga ggaactttaa
    11701 aaagctgtgt aaagaaaata tcaaaaaaag catgttttta gtgcttaaac aagtatttaa
    11761 ttcttatgta ccatggggaa ttttgactgg tattagacca gtcaaaattg tccacaattt
    11821 aatggataaa aagctagatg ataatagcat aagaaccatt ctaaaagata attattttat
    11881 aatagatgag aaaatagatt tagctcttga aatagctaaa agagagcgat tatttatgta
    11941 tcctatagat aaaaataaaa tttctcttta tgtaagtatt ccattttgtc caactagatg
    12001 tgtatattgt tcatttcctt caaattcatt aaaacaattt ggacatttaa aaagagaata
    12061 tgtttataaa ttgattgaag aaattaaggg gtttgccaaa gttattaagg atactaagaa
    12121 agaagttgaa actctatata ttggtggtgg aactccaact acacttgatg aggaagaatt
    12181 ggatttgctt ataaattcat tgttcaatga attagattta agtaaaatta aagaatttac
    12241 tgtagaagca ggtagacctg acactattac agaacaaaaa ctaagagtgt taaaaaaaca
    12301 caatgttagt agaattagta taaatcctca aactatgaat gatgatacct tggtaaagat
    12361 tggaagagag catagagtat ctgatttagt agattgcttt aatatggctc gtagaatggg
    12421 atttgataat ataaatatgg atataatttt aggtcttata gatgaagact taaatatggt
    12481 aaagaacaca ttggaggaga taaaaaaact ttctcctgaa agccttacag ttcatacatt
    12541 agccataaag agagcatcta acttaaatat aaatatggat aggtataaag aacatctaac
    12601 acaatatgaa gaaatggtaa aaatgataga tttatcaatg aaatatgcaa aagatatggg
    12661 attaaatcca tattatatgt acagacaaaa acatatgttg ggaaatttag aaaatatagg
    12721 ttatgctaaa gaaggatatg agtgcattta taatatacag attatggaag aaaaacaaag
    12781 taactatgca ttaggtgcag gtgctatatc gaaatttgtc tatgtagatg aagatagaat
    12841 tgaaagagta gaaaatgtaa aaaatgttga acaatacatt gaaagagtag atgaaatgat
    12901 aaaaaggaag aaagaagagg tatataaaaa tgttgactaa agctcctaga ggaactaaag
    12961 acataactcc aaaagaggct tacaaatggc gttatgtaga aaataaattt agagaaatat
    13021 gtgctttata tggatatgaa gaaatggtaa cacctatatt tgaacataca gaacttttca
    13081 agagaagtgt tggagatact acagatatag ttcaaaaaga aatgtattct tttaaagata
    13141 aaggtgatag agaaattacc ctaaagccag aaggtacagc aggtgtagta agagcattta
    13201 tagaaaataa attatatgca gatactcaac caacaaaatt attttatgtg acaccttgtt
    13261 ttagatatga aagaccacaa gctggtagac aaagacaatt tcaccaattt ggaatagaag
    13321 cacttggaag tgatactcct tcaatggatg ctgaaataat cgcactagct gttcaatttt
    13381 ttaatgaagt tggattaaat gatttggtag tgagtataaa ttcagttggc tgtccagttt
    13441 gtagaaaaga gtacaatgct ttattaaaag agtatcttga ctcaaaagct gatatattgt
    13501 gtgatacttg caatgagaga agagaaaaaa atccaatgag ggttatagac tgtaaaaatc
    13561 ctacatgtaa agaaaatatc aaagatatac catttattgc agaccatttg tgtgatgatt
    13621 gtaaatctca ctttgataaa cttcaagaat acttaaagga aatgaatata aactttgtaa
    13681 ttgataaaac aatagttaga ggcttagatt actatagaaa aactgctttt gaaattatat
    13741 ctaatgatat aggagctcaa agtactgtat gtggtggagg aagatatgat ggtcttgttg
    13801 aacaattagg aggaccaaaa ggtataagtg gaattgggtt tggactagga attgaaagac
    13861 ttttacttac acttgaaggt aatggaattg aaatagaaaa ccctcaatct acggatatat
    13921 ttatagtaac tataggtgaa gaagcaaata caagaagctt taaattgtta aaagacttga
    13981 gacaaaatca tataagtgca gataaagacc atatagagag aagtgttaaa gcacaattta
    14041 aatattcaga taaaataaat tcaaaattta caatagtaat tggagatgat gaacttaaaa
    14101 atgacactgc gacattaaaa aatatgaaaa catcagagca aactactgta aaattaagta
    14161 cattagttga agaattaaag caaaagctgt aaaataagaa agtatgatgt tttgtgtatt
    14221 agataaatat atagcattaa aattaaataa aatggaggta gtatcttgga aactttaaag
    14281 ggtttaaaga gaactcacta ctgtggagag ttgagagaaa aaaacataaa tgaagaagtc
    14341 gtacttatgg gatgggtaca gaaaaaaaga aatttaggtg gtcttgtatt tgttgattta
    14401 agagatacaa gtggattatg tcaaatagtt tttgatacag atgtagataa agaagctttt
    14461 gaaaaagctg aaaaattagg ggctgaattt gtaatagctg taaaaggaaa agtatgcgaa
    14521 agacaatcta aaaaccctaa tatgccaact ggtgatatag aaatatttgc aactgaactt
    14581 agacttttaa acaagtcaga aactccgcct atttatataa aagatgatga tgatgtttct
    14641 gaagcactaa gattaaaata tagatactta gatttaagaa aaccatctat gcaaagaaat
    14701 ttaaaattaa gacataaagt aatgaatatt acaagaaatt acttatcaaa caatagattt
    14761 tgcgaaatag aaacaccatt tttaatagct ccaacaccag aaggagcaag agattatcta
    14821 gtaccaagta gagttaatcc aggaaaattt tacgcattac cacaatcacc acaattatat
    14881 aaacaattat tgatggtaag tggtatggat agatatttcc aaatagttaa atgctttaga
    14941 gatgaagact taagagcaga tagacaacca gagtttacac aaatagactg cgaaatgtct
    15001 tttgttgagc aagaagatgt tatgagtatg attgaaggtt tgcttgaagc gatatttaaa
    15061 gaagttttag atgtagaatt ggcacttcca cttcctaaga tgacttatgc agaagctatg
    15121 tctaaatatg gttcagataa accagatact agatttgggt atgagttaac agatatatct
    15181 gatgttgttt gtaactgtgg atttaaagtg tttgctgatg ctacacaacc aggaaaaagt
    15241 gttagaggta taaacgtaaa aggcaaagct gacgacttta ctagaaaaca aatatcatca
    15301 ctagaagaac atgctaagac atatagagca aaaggtcttg cttggatgaa agtaggacaa
    15361 gaaggagtaa cttctccaat agcaaaattc ttcaatgaag aagaaatgaa cgctatactt
    15421 acaagaatga atgctgaagt aggagattta cttttatttg tagctgataa aaactcaata
    15481 gtatttgatg ctttaggaca agttagactt gaagtagcaa ataggcttaa tctattggat
    15541 aaaaatgtat ataacttact atgggtaact gagttcccag tttttgaaga agatgaagaa
    15601 acaggcacat tctcagcaat gcatcaccca tttacatcac ctatggatga agatttagac
    15661 aagttagaag aaggtgataa gtcatcttta agagcgaaag cttatgatat tgtattgaat
    15721 ggttatgaaa taggtggagg aagtgttaga atatcaaatt ctgatgttca atcaagaatg
    15781 tttaaagcac ttggttttac agaggaaaga gctaatgaaa agtttggtta tttattagaa
    15841 gcatttaaat atggtactcc tcctcatgct ggtctagctt ttggtcttga tagacttgtt
    15901 atgttacttg caggaactga taatataaga gaagttattg cattccctaa aaatcaaaat
    15961 gctgtttgcc ctatgacaaa tgctcctaca ttggcggaag atgagcaatt agaagaatta
    16021 agtataaaag ttgatataaa agataatgaa taatatgtag ttgcaagaaa ctaaaatgta
    16081 aaaagtctgg atataaaata tatttatatt cagacttttt ttattagaat tattttatat
    16141 tttttctcta tatattttca taaattatac ttatattctt aaccaatata attaaaatac
    16201 tatgtttaaa aattttacat aaacttggtt gttttagaaa ctacttctgt aacgagtaca
    16261 cgattttaat taaagaatat ataacaaatt tgaaataagt ctaatatatg acttaataga
    16321 ttaaattaaa ataataaatt tggactattt atctatttat aacttaaatt atatcaaatt
    16381 gataacaaac tagatattat acttttttgt agaatagaat tacaattata taaagaaaaa
    16441 ttctattaat agtgaaaaaa aagtagaaaa aagtagaaat tattctttta taaagaaagg
    16501 gagggataat attgcaagag agagaagaat tagttttaaa tccagttcca aaagaggaaa
    16561 gagttggatg gttagctcca ttgtttaata tgcttggttc taacatagct atatctgaac
    16621 taatggttgg gggaacttta attttaggaa tgactctttc taatatgatt attacatcaa
    16681 ttatagggaa cttaatatta gttgcaatta ttatgataca aggttatata ggttacaaag
    16741 aagggttaaa tacgtatgta ttagctaagg gggcatttgg agagatagga ggaaagtatc
    16801 ttatatctct attacttggt ataactagtt ttggatggtt tggtgtacaa gcaggggttg
    16861 caggactatc agtacaaaag atattcccaa gtgtaaacct aacattagtt acagttatac
    16921 ttggattatt tatggttgtt tttgcactgt atggttttaa agctatggct aaatttaatt
    16981 acttagttat accaccactt atgatattaa tggtgtgggg agtttttaaa gctttttcta
    17041 cttatggagt tgaagctatt tataattata ctcctcaaac aactatgagt atggttgaag
    17101 gtcttaatat tgtagtaggt cttgttatag ttggtgcaat tatatcacct gaccagttaa
    17161 gatatacgag acgagtaaaa gacatttgga ttatcagttt tttaggtcta gggattattt
    17221 cattattcca acaagttgca gctggtgtaa tgtctatggg agcacctact tgggatataa
    17281 cagaagtatt ggctaattta ggttttggat gggtagcgtt tgttatttta atacttgcat
    17341 cttggtctac aaacgtatca aatgcttatt ctggtggatt agcattaaaa actatttttc
    17401 caaatgtaaa aagaaatgtt ttgactttag ttgcaggtct aataggtaca ataatagcag
    17461 ctactggtat aatatttaaa tttcagtcat ttttatcttt cttgggaatc gcagttcctg
    17521 caatagcagg aattatgtgg tgtgagtatt attttataca aggaagaact tataagcata
    17581 gagaaggaat aaattggatt gcagtaataa gttggttaat aggttttgca gcgtcgtatt
    17641 atagttcaaa aataaatttc ttaatacctc cgataaatgg aattgtagtg agtatggtta
    17701 tttattatat attaatgaat tgttttggaa tcaaagataa ataaaggggg caattacatg
    17761 ggtcgtaagg tgaaaatagg tataattcaa caacacagtg tacttggaaa tgttaaaaaa
    17821 aatatagaaa aagcagtaga gatgatagat gacttaggaa aacaaggggc agatataatt
    17881 tgtttaccag aactatttgc tacaggatat aatttagagt ctttaggtgg agttaaaaca
    17941 ttagaattaa taagagaaca taataagtac atagaggaat caatgtcaga agctgctaaa
    18001 agaaataatg tatacctgat atctccatat ggaaccttag aaaaagggtc tacacatgtt
    18061 tataattcag cagttatatt tgatagaaaa ggtaagatta tgggagaata ctgtaaaaat
    18121 catctgtggt cattagaagc agtatacttt aaaggagggg aaaaagtaga ggtatatgat
    18181 gctgattttg gtagatttgg tgtaatgata tgttatgatg caggttttcc agaagtatcc
    18241 agagaattga cattaaaagg ttctgaaata atatttatac catctgcatg gagaatacaa
    18301 gatgaagata tgtgggattt aaatgtatct caaagagctt tagagaacac tgtatataca
    18361 gtaggtgtaa atttagtaag taatgactct aatttaatac tatttggaaa gagtaaaata
    18421 tgcaatccta gaggtactgt aataactcaa ttagatacat acagggaaaa atatgtatta
    18481 tcagagattg atttagatga aataaaaaag tatagggaag atatccccta tttgaaagac
    18541 agaaaaagta gctataacat ataattctat actataatac aagttcaata taaaatgata
    18601 aaaagaggat gtatcaatat taagattaaa tttattatac tttgatatat cctctttaat
    18661 attatcaact ttgatttaaa tatttttaaa atagttgata agaaagtttt tgaacaacaa
    18721 cacagcttta gattcatagt tttcgctaaa ccatgaaaga tttaaatatc ttttaaattc
    18781 aatatctttg atatgtaaca acttaatctc agggtcttga gataatcccc atgattttcc
    18841 acatataaat ccaactcctt gaagtgattt tataagagca taaattgtat atggtgaatc
    18901 agactcaaat gctattttgg gtttgaaatt agctgattcg cacaggatat caattacctc
    18961 tctataattc tcacccttcg ttataactat aaagttttca tctgaaactt cgcttagata
    19021 tacttcatcc tttaatgata aaggatggtt tattgaaact cctaatagta tttcttcgca
    19081 tgttaaagtt atgctatttt ctgagtttag ctttgtaaat gaagaagata tgtacaaatc
    19141 aaaatcactt tttttataag atgatggcaa agtatgtgac acattaaaag taatattggg
    19201 atatagtttt ctgaaattac tcaaaagtga tggtaacaca ttagccgctg caggagataa
    19261 tatctttact tctcctgaat tttccaaaga agtgtcttgt aactcctttt ttgaactttc
    19321 tactaaatct aaaatgctat ctactctttt tagaaacaat cttccatact catttaaaac
    19381 aatatatctt ccttttctgt caaataactg aactccaagt tctttttcta aggaagatat
    19441 agttttgctt aaagctggct gtgctatatt taacttgtgt gatgcttttg ttatgtgctc
    19501 cattctagca acagtttgaa aatacttaag atgcaataaa tccatagtat aaaatacacc
    19561 tcttttaata tgacttgagt tatatcaatt ttataatcaa ctccacatta tacattataa
    19621 tataaagttt atataataaa ctacaatata ttaaaaagtt atttaaattg ttatttatat
    19681 tttgtacata atctaattaa aaaaagtaac tttaaatggt taactaagga aatattgtta
    19741 caaactttag ttatataaaa aattcgtttg atgagcaaag tattaatgtc aagtaagaat
    19801 aaaagcttat tagataagaa aaataatatt ttataataat gatgttactt aaattattat
    19861 gttatactat tttaaatagg aaacatttag atactataat tttaggggga gtcaagatga
    19921 aattaactaa atcaatatca tcaatgctgt gcttatctct agttttaagc ttatcaccag
    19981 tagcatatgc aaatgaaata agtagaacaa cagttgagaa tactttagta ggtcaggata
    20041 gatatcaaac tgctgttgaa gtaagtaaaa aaggatggag tagtgcaaat gaggctgtag
    20101 ttgtaaatag tgaatctatt gtagatgcat tagctgtaac accatttgct aaattaaaaa
    20161 atgctcctat attattgaca gaaaaaaata atctaaattc acaaactgag aaagaattaa
    20221 aaagattagg agttaaaaaa gtttatataa ttggtctatc tagttctgta tcaaatgatg
    20281 tgcaaaaaca gttagaatct aataatttaa ctactgatag agttggtggt agtgatagat
    20341 accaaacagc tttgagtata gctaaaaaaa tagaaggatt aaaagatata tcagaaatag
    20401 ctgtagtaaa tgggtacaca ggtcttgcag atgcagttag tatagcttct gtagctgcta
    20461 ctaatggaat ggttatactt ccagtatctg atgcatctgg tatatctagc tttaaagatt
    20521 ttatagaatc taagaatata aataaatcat acataatagg ctctactaat gctgtatcag
    20581 ataaaataaa acaaagttta cctaattcag aaagaatagg tggagcagat agaaatgaaa
    20641 ctaatggtaa agtaatagaa aagttttata catctaataa attaaacaat gtatttgtgg
    20701 ctaaggatgg aatgaaaaat caaaatcaac ttatagacgc tttagcagta ggagtactag
    20761 catcaaaaga aaattctcct gtactaatcg taggagaaca attaagtgca acacaaaaga
    20821 gtgtattttc gaataaaaat gctacctcta taactcaagt tggtagtgga ggaaatgaaa
    20881 atgcatttaa agaattaaaa gatttacagg caaatagtaa caataagaga gtaatgtatg
    20941 taactaatac agataaggta aatataagaa atgatgctac aatagaagct tctgttatag
    21001 gatatctaaa taatggtgat gaagtagaag ttttagatgt gttaaaaact ggatgggtag
    21061 aaataaaata caatgaaggt attggatatg ttagtgggtc ttatttgaca aataataagc
    21121 ctgataatag taatgaaaat ataaaaataa agtatgtaaa agaaaaagat ggtctaaatg
    21181 ttaggaaagg accaagtaca gaagatgaaa aaataggtca cctttcatat ggaagcaaag
    21241 tagaaacaat agaaatgttt gctactggat gggtaaaaat aaagtataat ggtggatatg
    21301 gttatgtaag taatgattac ttatctgata caccagttat ttaattataa gtaacaataa
    21361 aaaaactgct ttaatttgta aagcagtttt tttataaaaa tttaagtcta ataactattt
    21421 ctattttgat accttaatcc tgggatttat tattatggat attatcaaca tacaaacact
    21481 ccaaatccag tgtctaattc caaacttaga tattaataaa ttcagtcctg ctatggtaaa
    21541 taagcagtat ttaattacaa ttaaaagtgt atttatagaa agccccttca tagaaagtat
    21601 cctaaatcca ataatagaca ttctaagtcc tataaatgcc aataatatac ctgcaatatc
    21661 ctgaattaag taatagtatt caaatttcat attcaatcaa ctcttctctg agcagtatca
    21721 aatatttcta actcttcaat ttcatcatct gtgtatgtaa gcatagtaaa accagttaca
    21781 ccataaataa atgaaattat aggtgtaatc caacttaaga aacaatatgg aataaattct
    21841 agtgggctta caccaagagt ctgagcacag aaaacagcat ttgaattcca tggtattaat
    21901 ggaccaccaa gtgtagctgt atcttctata attctagata gattttgtgg ttttaattta
    21961 aattctttgt atattggaga cattagagtt ccgacaaata cagaagtgaa catcattgat
    22021 gaactaaatg catttgcaaa gtaacttact ataaaagtag acccaaccaa tttagttctt
    22081 gattttccta ttttagttat aagaggttga agcagtactt ctaagaatcc agttttttgg
    22141 agaataccag caataacgaa aactacaaat actagtaaaa cggtttcagc catactcatt
    22201 attccaccac gatttaatag tttgtcggca tagacaaatc cagtatctat agtaaatcct
    22261 gatagcatgt aatttaaaac atcacctatt ttttctcctt cttgaaatac tgctataata
    22321 agtcccatga tagctgaact tagtatagat ataatgggtg gcttttgtag caataaaagt
    22381 aataataaaa atattattgg aatcattgct actaaaccaa tatgaaagtt tgaatttaat
    22441 acatccttga tttgatttat ttgaacatag tctatagtat tatttgaata cttaaatcca
    22501 attactgtat acagaactat acatattacg tatgcaggtc ctgtggtata aaacatatgc
    22561 ttcatatgtg taattacatc tgtcttacaa actgctgcag ctaagtttgt agaatctgat
    22621 agaggtgaca tcttatctcc aaagaaagca ccagaaatga ctgcaccagc tgttaaacca
    22681 agaggaatac ccatactagt accaatactc atcattgcta taccagaagt tccaagagtc
    22741 ccccaagatg taccagtagc aacagaagtt aatgaacata cgattaatgt tgtaagcaga
    22801 aaatatttag gggttattat tgttagtcca ctatatacta ctgttggtat tgtaccagca
    22861 gctatccaag ctgctattag tacaccaaca gaaagtataa ttaagtttga ttgaaaggca
    22921 tctttaccaa cttcaaatcc aaaagcttct atttcatcat tagtataacc aagtttcata
    22981 gctgcaggaa ctactataag ccatgaaagt aaaaacattg tagttataga agcattaaat
    23041 acaactatac caactgatgt tagagctatt attgcaagca ttactaacaa ggaatatcta
    23101 aatgaaggta tttttctttc tgtattcatt ttcatagtgc aaattctcct taaatatttt
    23161 tagttaattg cattttttat tcttataaga gcttcttcaa gcatacttct tggacaagct
    23221 aagtttattc tttgataccc acttcctcca atgccaaagc tgtttccttg atttagagca
    23281 actttacctt tttgtacaag tatactttct agttcctcat cacttaatcc aagtgcacta
    23341 aagtctaccc ataataaata agtaccttct ggttttctaa ctttaagttt tggcatattt
    23401 tcatttatat attttatagc aaaatctata ttagattcta aatattctaa aaatgattct
    23461 agccaagatt caccattatt atatgaagct tcagttgcaa ctaagctaaa gcaattattt
    23521 ctctttatat caattctggt aaaggcatca tctaacaatt tatagtcttt ttcatcaggt
    23581 agtattacat atgatgattg aagaccagct atattaaaag ttttagttgg agccatacaa
    23641 gtaattgtat ttttttcaaa ttctttagaa atagatgcca taggaatatg cttatgtttt
    23701 tttaatataa tgtcactatg tatttcatca gatatgattt ttacattatg cttaagacaa
    23761 atatctccta gtttttttaa ttcatctttt gtccatactc taccaactgg attatgagga
    23821 ttacaaagta tgaataattt tacatcttta atcttgtttt caatgtcttc ataatccatt
    23881 atgtagtttc cattttctag tttttgtaat gggcttataa ttagttctct attgttattt
    23941 tttacaacac tattaaaagg gctgtaaact ggttcttgta tcattatttt atcatttgct
    24001 tttgtgagtt catttattaa aagacttata gctgggataa cacctggact ataaattaac
    24061 cactcacttt ttattttcca gttatgtctc ctatctagcc agtttactat agattcattg
    24121 taagaatctg gtcttgttgt gtagccataa atctcttgtt ctagtctatt ttttaaggaa
    24181 tcaattatac aaggtgcagc cttaaaatcc atgtcagcca cccacatagg aagtaaatca
    24241 ttagttccat actttttttc catttctgac catttagatg aaaaattatt gcttctatca
    24301 acaatctcat taaaattata attcattgta aatacctcca gtaattttaa ttttatgtta
    24361 ataatattaa gcaaacaacg tgccaatatt gataataaaa aatatttatt cacaagttaa
    24421 tatttaatat taagtaattt cagagcatag ataagataaa aatattaatt atacttaaaa
    24481 ctttgtggaa aaggttattt ctaataaatt tttaaatata tgaaaatgta attaaatagg
    24541 ttaaataggt ttaactgttt aacttgttag acctatttat attttttaga aattctactc
    24601 cttttgatgt tataattgaa cctccacgac caacaagtat gtctacaagc ccaaagtttt
    24661 gtagttctaa aagcatattt ctaatctgtt gttctgtcaa aaatctattt tcctctaaag
    24721 ctattttaca taagcttttt ctacctattc tttcttttaa atcataagcc ttttttaaat
    24781 tatccaatat aaatatgtat tcatctagtg accttttaaa attatagtcg tatttcatga
    24841 tgtttttgtc tttgccaatg ttgaattgat acttttttat attatcactt actttattac
    24901 aaacggttct actataattt gaatctatag tatctaatat atattcaggt aaatcacata
    24961 tttctataat atctttttcc aagtaacaga agtattctcc taagtttctt aattctctta
    25021 catttccttc ccatctatac attttaaaga tttcttttac ttcctcactt agaataaaat
    25081 tacatggaat atcatatttt aatgaaccaa aaatttcaaa tatatcagca ggtctttccc
    25141 taagtggatg tattttaagg ggaagaacat tcaacctaaa atataaatct cttctaaaca
    25201 tatttttaga tacaagttct tttaaatttc tattagtagc tgcaattatt cttacatcaa
    25261 tgtcaatgac attattagaa ccaatacgtc gtacttgttt ttcttgtatg actctaagta
    25321 atttagattg agaatttaaa tccatttctc caatttcgtc taaaaatata gttccattgt
    25381 tggctaattc aaataaacca attttcccac cttttttagc accagtaaaa gccccttcat
    25441 catagccaaa taattcactt tgcagtagat tttcttgaaa agtactacaa tttactgcga
    25501 caaaaggacc atcttttctc ctagaagcac tatgaatgga ttgagcaaat aattctttac
    25561 cagtaccact ttcaccaatt ataaggatag atgaatttga ttgagccatt ttattagcaa
    25621 ttttttttgt attaatagtt tgtatactag aacctaatat gtcatcaaat gtgtatttac
    25681 tgatattacc agagtttact agttgtgctc ttaatttagc ttgctttttt tctaattgag
    25741 aaaactttgt catctttagt ataaatccat caaatacatt taattgtata tgtttaatct
    25801 ctaaatttat atcaatatga tttattttaa tcaatttctc aaaaaatgga gttttattgg
    25861 tcaatatgct ttgaaaattg atgtctttta tgcaatcacc aacaaatgag tcaatcattt
    25921 catttgcatt tatagacaaa atttttctag ccatatgatt atagaattga attattccat
    25981 cgttatttgt gcatataatt ccatcatcaa ttatagatag caacaaatca aattgtcttt
    26041 caaacttatt tgcatctaac aataactcac ctgtactata acttgtagga attatttttt
    26101 ctacgtattt ttttattagg tcatctttta ttagatgttt taatttggtt tttgtagcaa
    26161 tttcaacaat acaacttaaa tctattattc tgtattttaa atctataact tcacaatttt
    26221 taaacttagg caagattttt tcgccaggag ttaggattac agcatctgtg ggagtaaact
    26281 caatctctgg atagcagggc aggatattta tattgtctat tcctaatcta tgtattaaag
    26341 aaattgtttc tattgccata tctttactta agttatatac ataaataatt ttatcttttt
    26401 ctaatttgga tatcatatca atactgttct tttcaaatgt caacttagga acaatgattt
    26461 gagcatcata gtgagataat tttactattt catcatattt taaataagca gataggagta
    26521 taagtttttc tttaaaaaac tttaaatttc cctcttcaaa tgagtataaa tttatcttta
    26581 gattatcacc taagaattgc cttatttgat tttcataaat ttctccagcg tcttttttta
    26641 aacttacaat agctaattcc atttgtttca ttttaacctc tcatacaaat ttgacttatt
    26701 ttagattaca taataattaa attagattaa ataatattat ttaattatta ttataaattt
    26761 attatagact acacattgaa gtaaaagaag tagctaaaat tattcctttt atttttgttt
    26821 tttgtatcta tttggtataa tagtattaga gcgtataata atttaggcaa aattttttaa
    26881 ggaagactag aggagaatat aaaatgaaaa aatgtcttta ctgtaatagg gatataaagt
    26941 caccaaataa taaattagct ataaaatatg atgacaatat gaatatatat ccatgtcgtg
    27001 cagaatgtaa ggaaaaaatt gaagaatata tagcaaaaaa acctacatat aagtttataa
    27061 atatattaga ggttctttta ggtgtgggcg gaatattctg ctttttgagt aaatggacta
    27121 ttgcagccca agttgtactt ggaattgatg cactggttat atttttattc ccattggcag
    27181 gatttaaaat gaatggaaag ttaagtatgg aacaaactaa gaatcaaagt aggtctattg
    27241 caatatcaat tttggcattt atagtaataa taactgtatt atattttaaa caagttggta
    27301 agtagagcat aattgtataa gacatcatta gagtatgagg tgagtttatg gaagaaagaa
    27361 aattagcacc aaacaatgag agggaagctc tcataaaaag attaaaaaga atagaaggtc
    27421 aagttaaggg tatacaaaag atggtggaac aagaacgtta ttgtgttgat atattggttc
    27481 aaatatctgc tataaggtct gctataaata gagtaggcac aattatctta gagaatcata
    27541 taaaaggatg tgtagcagaa agtattaaac atgatgatgc tgataaaact gaagagatga
    27601 taaaagagct tatggatact ataaataaat ttacaaaata aggaggcggt tagttatgaa
    27661 tcaacaagga tttattatgg aaattgttga tgatagaact gcaaagttaa aaatgcaaag
    27721 acattcagct tgtgctgctt gtggaaaatg tgctactaca aattcagcag agtcaaaaga
    27781 aatattagta gaagtagaca acacaattgg tgcaaaagtt ggtgaccatg tagaagtgag
    27841 tatggataat atgaatgtat taaaagcagc tgtaatggct tatataatac ctcttatagc
    27901 acttcttgta ggaacaatag ggacttatta tatattgggg gcaattggaa tgacaactgg
    27961 agttgaagcc ataagtggat tagttggaat ttgtcttaca ataatctgtt atttatactt
    28021 aaagaagaat gacaaaaaat ttagagacag tagagaattt ataccagtaa taacaagaat
    28081 attaattgac ctttaagata attataatta aaaaatagca atgtactttt taaataaata
    28141 ctcaatacgt atttgtagag tttaagtaca ttgctatttt tatattttta ttttgatttt
    28201 ctgtaaggaa taaagttttt tctgattttt aaaagattca atgttcttac aaaagaagtt
    28261 acaactaata caccaagtgc tattgcagca cttagaaata gcgcatctgt accttttgac
    28321 atagccaaag aataattttt ctgaatcaaa gacaacattg tattatatag cccaattcct
    28381 ggtattagag gaagaatacc tggaattacg aacacaatag ctggttgctt tagttttctt
    28441 gctaaagtct cactgcacgc tgagaccaaa gcagcagcga taaaacctgc aaaaactgca
    28501 tttgtagtaa aattaaataa ataaacatat acaatccaac caattccgcc agttactcca
    28561 gctggaagaa gtagatatag tggagcatta aagaaaacag caaatccagc agttgcaaca
    28621 cctgaaaata aaaaatgcaa aatcatagac atatcagtca ttagaaagga cctcctattc
    28681 tataccatgt atcaaggaca aaaccaactc cagaagcaat tgcaataata atcatagcag
    28741 catcaaaagc acgagaaatt cctgatatta aattccctga tattaaatct ctaatagctt
    28801 ttataaaagc aactcctggg agtagtggca ttatagcgcc gactataagc attttagggt
    28861 catctaaaat gcctatttca actagtagaa ctcctgaaag tgcaattaaa aaagaagata
    28921 ctaaacagga aaatgcagat attgcactta tcttcatcgt tttatcataa gtaattgcag
    28981 caaagattgc tattataaac gtaagtataa aatttaatac ataattccct ccaagcaaac
    29041 aagcaaaaca agcagatgca attcctgtac aagagtaaaa taaccataaa ggataaggtt
    29101 ttacatcttg gatttcataa agacgtgcaa tggcattatc aatatcatac tctttgtcac
    29161 taacaaattc tcgagaaaaa ctgtttaata aagaaatctt atttaagttt atacctcttg
    29221 attgaatcgt tttcataaac gaaaagccat caaatttctc atctgaaata attattactg
    29281 taggagttgt aaaaatattt acatgataaa accctcttga cctgcatatt ctaaggacac
    29341 tgtcttcaac cctagatgtt tcagctccat tttttatcat cagttcccct acaaatagag
    29401 ctagttttag aatatttttc ttatattcaa gttcctcatt ttgattcata aatacacctc
    29461 caaattaaaa aacgtaatat tgaaattata tcaaattttt aagtaatatt ccttaatatc
    29521 tatacaaata tattatcttt tttttgtttc aaaatagtca catttaaatt atatccaatt
    29581 aaaagaatta gacaatatta tttattatat tttaacttaa aaataaagaa agtaactatg
    29641 ttaaatatgg aaaaatggca aaacacttga ctattaattg gtaaaggact ataataataa
    29701 attatcatat tgataaaaac gaaaattatg aaatgtctgt aaaaataaaa tttaaataat
    29761 aactaggagg agtaatatgt ttgagaactt gaaaaaagcg gaccctgaga tttatgaaag
    29821 tatgaaaaga gagttaaaaa gacaacaaag aaatattgaa ttaatagcat cagaaaacat
    29881 agtatcagtt ccagtaatgg aaactatggg aagtcatctt acaaataagt atgcagaagg
    29941 ttactcaggc aaaagatatt atggtggatg tgaatacata gatgaagttg aaactcttgc
    30001 aatagataga atcaaaaaaa tatttggagc agaacatgct aatgttcaac ctcactctgg
    30061 agcaaatgca aatattggtg tatattttgc tatgttaaaa ccaggtgatg ttgttatggg
    30121 aatgaattta tctcaaggtg gacatttaac tcatggtgct ccagttaata tttctggtca
    30181 atattataaa ttttatgaat atggtataga taaagacagt ggacttatag attttgatga
    30241 agttagaaag atagcacatg aagtaaaacc taaaatgata gttgcaggag caagtgcata
    30301 tcctagagaa atagatttta aaaagtttag agagatagct gatgaggttg gagcattgct
    30361 tatggtagat atggctcata tagcaggtct tgtggctgct ggtcttcacc aaaatccatg
    30421 tgaagtggct gattttgtta caacaacaac tcacaaaact ttaagaggac ctcgaggggg
    30481 tgttattctt tgtaaagaaa aatatgctaa agacatagac aaagcaatat tccctggtat
    30541 tcaaggtgga cctcttgaac atataattgc ttctaaggca gtttgcttta aagaagcttt
    30601 aagtgatgag tttaaagaat atcaggtaca agttgctaaa aatgcaaaag ccttagctga
    30661 agaacttatt aaaagagatt ttaaattgat ttctggaggt acagataatc atttaatact
    30721 attagatttg actaataaaa atataactgg taaagcagca gaaaagagat tggatgatgc
    30781 ttatataaca gctaataaaa atacaattcc atttgaccca aatggtgctc ttattacaag
    30841 tggaataaga cttggtactc cagctgtaac tactagaggt atgaaagaag aagatatggc
    30901 aataatcgct gaggctatag acctatgttt aacttatgat gaagaatcta aggctagaac
    30961 attggttgtt ggtttaactg aaaaatatcc tttatatgaa gaatataata ttatggatta
    31021 attataaaga acaactaaat aaaatacatt taaataatta caatgattgt gtatgtattt
    31081 gaatatagtt agatataaaa aaggtgtctc aaaatgaact ttttagttta tgaggcactt
    31141 ttttgtatgg aatcgaacat atttgtgtta tttaaataat aaaaaatatg gttatctcta
    31201 tttttagatg gtctctttgt attttttaat taaaacttta agatttctta agaattatac
    31261 taggtttttc taaagattta aagtctatac ttataagtat agtaatcata tgaaaagaaa
    31321 agctttaacg aggagggaat cttatgaagg tattaattct tacaggaaaa tttggtatgg
    31381 gtcattactc agcatcaaat tctttaagtg aagatataaa agcaaaattt gataattcag
    31441 aaataataat taaagatata tttgagtaca ttatgcctaa ctattcagat aaaatgtaca
    31501 agactttttc tattcttgta aatcggggaa gtagtcttta taatttattt tataaatgtg
    31561 ctgaaaatgg aaagaaagat ataaaattta ctttttcaga ttatttttta aataaattag
    31621 atacattact tcatgaagta caaccaacag tagttatttc aacttttcca ttttgttctc
    31681 aattagtatc aagatataag gaaaaatata actcaaatct tcctttaata acatgtataa
    31741 ctgatataag tagtcactca gaatggatta gtaaaaatac agactgttat ttagtagcta
    31801 gtaaatctac taaggaagaa cttgttttta aaggaataga tgaatctaag attaaagtta
    31861 atggtattcc agtaaaaaaa gaatttaaaa gaatagaaca tgtaaatcat tcaactaaaa
    31921 aaaatattct tataatgggt ggaggattag gattactgcc taaatcagaa caattttaca
    31981 aagaattaaa tagtttagaa ggagtaaaaa ctactgtaat aactggtaat aataaaaaaa
    32041 tgtactataa actatatggt agatatgaaa atatagaggt agtaggttac accaatgaag
    32101 tatataaata tatgaaagat tctgacctta ttatttctaa accaggtgga ataacactat
    32161 ttgaaacaat ttattcagaa cttccaatat tggcatttaa tccattttta caacaagaga
    32221 tagataatgc tagttttata ttaaataatg aaattgggag aatacttggt aaaaataaaa
    32281 aatactatgt agatgagata aaagatttaa tttatgatga tgctactctt aaagaaatga
    32341 gcagtaacat gaaagaatta aaaaaacaat ttgataataa taccttagag aatattttat
    32401 tttcacttga tgaacaagga gcgtgtagag aatgtatgta gtaggtttaa ttatcgttgt
    32461 tgcattgatt tttttagtcc attcaattat acctacatac tataataaac tattaaataa
    32521 agaggtatta aaaaatatgg ctggagaaaa tgaaattgca cttacatttg atgatggtcc
    32581 agataagaga tatacagaaa aattactaga tgtgttaaaa gaaaatgata tacaagctat
    32641 gttttttgta gtagcaaaga atgctgaaaa agagcctgaa attataaaaa gaatgttgag
    32701 agaaaatcat atagttggtt tacattcatt ggaacacaga aatgcatggc tttatagtta
    32761 tagttatgtt aaaaaagatt ttattgaaag tacaaatata atgaaaaatt taggtgtaga
    32821 tgtaaactac tatcgtccac catggggtca tactaatata ttttcaaatt catttgttaa
    32881 aaaatataat ctaaaaatga ctttgtggga tgtaatggca gaagattggg agaaagatag
    32941 tacagtagac attattataa acaagttaat gagtagaaca aaagaaaatt ctataatttg
    33001 tttacatgat gcaggtgaaa attcaggtgg tgcagtaggt gcacctgagc gtactattga
    33061 agctttaaag atagctattc caaaacttaa agccagtggt ttaaagttcg taacaccaga
    33121 aaggatgtag ttatgataag taaaactgaa aagaaaaata aatgcctagg tagtgtagct
    33181 tttttagttt tactaatggg cataacagga tattttgttt ttagagggca gtcagttgaa
    33241 tcattgataa aatcattaaa aggtgcaagc cctatgttta tactaattgg atttgcaatg
    33301 atgtttatat atgttgcctg tgaaggtata aatatatact taggtatgaa agctttaaac
    33361 caaaagacta ctcttttaaa atgtatggga tatgctttta ttggatttta ttttagttct
    33421 attacacctt cagcatcagg tggtcaacca gcacaagttt attatatgaa aaaagacgat
    33481 ataaatatat catattcttc acttatacta ttagtcattg ttgttattca ccaagtagtt
    33541 atactggcat atagtggaat aatgttcatt atggaacgtg agtttatatt aaataatgta
    33601 agtggtatga acattttact aatatatggt gtaataacaa atgttgctct tgtcattgga
    33661 gtaattgcaa ttatattttc aaaaaaactt gtaaacaatt ttataatatc aataacaaat
    33721 ttattaggta agttaagaat aataaaagat gttgaaagtt caagaaaagt tataatatct
    33781 caaattgaag agtatgttaa aggtgctcaa tatataaagc agaatccaaa acttgtagtc
    33841 caaattttag tcataactat tgtacaaata acagctatgt ttttagttcc tttctttgta
    33901 tacaaagctt ttcatctatc aacatatact gtatttgaga tattagcgat acaatccttg
    33961 cttaatattg ctgtttcttc attaccattg ccaggggctg ttggagcttc tgaaaatagt
    34021 tttatgacat tatttaagat atttttccct ggtcacttat tagttccggc aatgttactt
    34081 agtaggggaa taagttttta tgcatttgta gctataagtg gattgatatg tatagtagta
    34141 catgtaaaat cttctgaaaa agtaaaagca gtgaaaaaag ttgcttatgt aagataatat
    34201 tatatagatt atttttatta atatgtcaat ttgtgtgtat attttaatta agctttaaac
    34261 atatttacag gagtagaaac tattaaaacg tgtgacattg ctaagctttt gctaataaag
    34321 tataatattt tatatagttt gaaatattta agaaatatag caaaaaaagg agcttggtga
    34381 tgaataaaga gattccaaaa aaaattttag tagctgatga taatcctgag attagggaaa
    34441 tcgtagaaat acttttaact ggtgaaggtt atgaagttat tatggcaacg aatggagaag
    34501 aagctgtaaa cttagtagat agtacgattg atttgattat tttggatgtt gtaatgcctg
    34561 taaaaacagg atttgttgca tgtaatgaaa ttagagaaaa aacgacagca ccaatattat
    34621 ttttaactgc aaaaactcaa gactctgata aagttattgg attttcagca ggaggagatg
    34681 actatttatc aaagcctttt tcatattctg aactaatttc aagagttaag tctctactta
    34741 gacgttatta tatataccag ggaaaggaaa aggaaagaga gccaaatata ttaaaaacaa
    34801 aagagttatg tgtcaattta gatactcaag atgttacact aaatggagaa cctgtgtttt
    34861 tgacaactat agaatatagt atattaattc ttatgttgaa aaatagaaaa aaagtttttt
    34921 cgtctgaaaa tttatatgaa actatttggg atgagcagta tttttataca gcaaataata
    34981 caataatggt tcatattaga aatttaagaa aaaagttaga aaaagaccaa aaaaatccac
    35041 aatatataaa aaccgcatgg ggaaaggggt attatattga ttaacaaaat taagccatct
    35101 aagttgggag ttatactatt gatatatgta gtaatctcag ctgcagtggc tttcatttta
    35161 aatataccat tttactctgc tgttgaatct atagtaaaag atgtatcaga agggaaagta
    35221 tattttaaaa gagaagtaaa tcaacttatg gataattttc aaacttatgt gaataacaat
    35281 ggtataagag cagatgatgg agatgatata gcaaaatgga atagtgataa ttggtttgtt
    35341 tctatgaaga tatataaagg aaataaggtt ttttatgata cattatacta tcctaatgaa
    35401 gtacctgata agagtgagga aaatgagttt tatagcagtc ccatggctag aaactatgag
    35461 atacaatttt ctgatattaa agctaatata gttgtagtag ctttatttag atttagattt
    35521 caaaatttta tagaagcaat ttctattaca tttatggcac ttacatttat tataacattt
    35581 ttactgttgt ttagtagaaa gataaagtac ctaaggaaaa tagaaatggg tataaagata
    35641 gtagagagtg gcagtttaaa gtatagaata ccagtaaaag gtaatgatga attatcatca
    35701 cttgcatcta gtattaatga gatgagtcat acattggaga atcaacttat aaaagaaagg
    35761 gagatgaaga ataaaaataa agatatagta acttcaatat cacatgatgt tagaactcca
    35821 ttaacgtcag taatttgtta tttagatttg attaaagacc ataaatacaa tagtcctgag
    35881 caattggaac aatacataaa aaatgtaagg tctaaagcat atcaaataaa agatttaaca
    35941 gatgaccttt ttacgcattt tgttaattct ggagaggaag tttcaaaagg gtatgaaaaa
    36001 ataaatgcta atgaatttat ttttcaatta cttttagata gttcatatac attggaggag
    36061 aaaggatttc atataacaat agaaaatgaa ctttataacc aatatcatat ttatgtagat
    36121 gttacacaat ttagaaggat atttgataat ttatgttcta atataataaa atatgctagg
    36181 aaagataagc ctatagtatt tttaattaaa gtagaggatt ctaaattact aattatacaa
    36241 aaaaatagag taagtaattt ttctaagaat gtagaaagtt ttggaattgg tataaagaat
    36301 tgtgagaaaa tagttgcata tcataatgga agcataaatg taaattcaga tgataaagaa
    36361 tttgctattg aaatatctat tccagcaaaa gaatcataaa aatttaaatt atatattgtg
    36421 taattgctat tatatattgt gtaattgctt atgtaaaatt ctatgaagca ggaagcatgt
    36481 gactttagtt atgggatgtt caatattgtg tacctctagg caagtaacaa gtttacttac
    36541 aagaccatat aatattatta tcttaaaaaa aggggacata ataatatggt tttgacagga
    36601 gcacattata tttacattct atttatggta attatattaa ttactatgat tatgaaaaag
    36661 gatacaatag taccatgtat attaggtgtt ttttttatgg ggttgttctt tgaaaaaaac
    36721 atttttggtg ctataacagc agtgtttaac tcttttatta tatctttaaa tgagttgggt
    36781 ccaattatct tgattatagc tattatggtt gcattatcta aagccctaga agccaataat
    36841 gctatacagt atatggtgag accattttcg agagttataa aaaattctaa tacagcattt
    36901 tttgtaactg gatttgttat gttagttctt tcttggtttt tctggccaac tccagctgtt
    36961 gccttagttg gagctgtatt tttacctgtt gctatgagag ctgggctacc agcaattggt
    37021 gttgcagtag cattaaattt atttggtcat ggtcttgcct tgtctacaga ctttgttatt
    37081 caaggagctc caagtataac agctggtgca gcaggtgttg cagtatcata tgttataaat
    37141 gatggtatga ttttgttttg ggtgatggga atcgtaacca tatctatggc attttataca
    37201 ttaaaaagag atattaacaa aggaatgttt agagaagaac taaaaggttt tgaaagtgaa
    37261 gaagtagaag agttcaatgg aaaatctaaa atagcaacta ttttagtagc tctgggattt
    37321 cttgctgata taattgctat gtatatattt gacctaaaag gaggagatgc atcagctctt
    37381 ttgggtggaa cagcagtatt tttaataata attataaatg ttataaattt tggcaaagat
    37441 agtcttgaaa atgtatgtga gaatataatt gatggttttg tgtttgggat aaaaatattt
    37501 ggagcaatta taccaatagc agcatttttc tatatgggtg aagttgcacc attgacaggt
    37561 gtatttggaa aagtattagc accaggttca caaggtttat tatcagatat aggcatagct
    37621 ctttctcaaa caataccact taataaattt gccgtatcag gtatagaaac tgtagtggga
    37681 gcaataactg ggcttgatgg ttctggattt tctgggattt cattagttgg ttcacttgcc
    37741 tctgtatttg gaactgctat aaatgcaagt gttggtgcac tagctgcatt aggacaaatc
    37801 agtggtatat gggtaggtgg aggctgtcta gttccttggg gtcttatttc agcagcggca
    37861 atttgtggag ttagtccaat agaattggca aagcgaaact ttgtcccagt tataaccgga
    37921 ttggttgtaa caaccatagt agcaatgttt attatttaat aaatcgaata aatctttaat
    37981 tgtataaatt aaaaatagat gtgtagctat aatttattat aattgtttta ctaattctaa
    38041 gccatacatc tatttttatt gatattttta ttagtattct ttaataaaat aatatttttc
    38101 attatactat acataacatc taaatataac atctaaaata ctgttatcta tatttaatat
    38161 cttgccaaat tttttgatgt tttattgccc tgtcccacca acatatataa gctatcacaa
    38221 gtttttacct atactatcac caaacaaaaa ttctaatttt aatttattat tttataacct
    38281 atattatatt aactatatta gtttgtagac tttagatgta attataaata ttaatactcc
    38341 aagagttcaa attcatatcc tagttttctt gcatttgata taaaatcatt taaaatttct
    38401 gtactagttt tggaaactgc atgtagtaac aagatagaac catcatgtag attatccatt
    38461 attttttgcg ttgcttcttc atgtgatggt tgtttatcag tatcccagtc tctgtatgca
    38521 aaactccaaa atacagtctt gtatcctaaa tttttagtca ttgccaagct tttttctgag
    38581 tattttccca taggaggtct aaaaaattta accatatctt tcccagttac atctttatat
    38641 aacttttcaa catcatataa ttcgtcatta aagtttttta gattgcttgt ttttgttggc
    38701 atagatggat gggttttact atgatttcca actatatgtc cctcagaaac cattcttttt
    38761 actaaatctt tattttcttt tatatatgga gctgtaacaa aaaaaactgc tttaacctga
    38821 ttttgtttta gagtgtcgag tatttttgta gtgtatccat tttcataacc ttcatcaaat
    38881 gtaagatata gtgttttttg accatctttt gtaggtccat tgtatagggc atcatagtca
    38941 gaaaatttaa attcaatgtc cgtatttact tctggtgttt tatgcttatt gtttggtata
    39001 taaaaccaat caagagtagt gttatctaaa gaagatacat ctaatttatt attatcatta
    39061 ttagtagtgg cttttgatgg tttatctgtt ccgtctgaaa ctattacatt ggaagtatct
    39121 ttaccactat ctattttttc cttatcttct tgtaactgtg tttccttatt ttgattttcg
    39181 ttttgattgt tttgtgaatt tgaacatcca acaataaaaa aaatagaaat agaaagtagt
    39241 gttaaataat gcaatacttt tctaaacatt ttgaagctcc tttctaaatt aataataatt
    39301 gcatataata ataattatat gattaattac aattgcatat agtattatta taattggaaa
    39361 ttactattaa taatagttat atctttaaaa ataaggtcac aatagtatga caaagggtga
    39421 ataatcaata aatttaaaaa aaaatgaaaa aaatagttga ccatattatg agaagatgtt
    39481 attataataa atgtgctcaa caagaagtaa caaaataaca agcacaaaga agttaattga
    39541 at
//
LOCUS       DAYATN010000020        39377 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.020, whole genome shotgun sequence.
ACCESSION   DAYATN010000020 DAYATN010000000
VERSION     DAYATN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39377
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.020"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(86..173)
                     /locus_tag="ACGI8A_001048"
     tRNA            complement(86..173)
                     /locus_tag="ACGI8A_001048"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Leu,seq:caa)
     gene            325..2973
                     /gene="polA"
                     /locus_tag="ACGI8A_001049"
     CDS             325..2973
                     /gene="polA"
                     /locus_tag="ACGI8A_001049"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFH0179056.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            2982..3584
                     /gene="coaE"
                     /locus_tag="ACGI8A_001050"
     CDS             2982..3584
                     /gene="coaE"
                     /locus_tag="ACGI8A_001050"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFH0179057.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            3577..4131
                     /locus_tag="ACGI8A_001051"
     CDS             3577..4131
                     /locus_tag="ACGI8A_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFH0179058.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            4131..5846
                     /locus_tag="ACGI8A_001052"
     CDS             4131..5846
                     /locus_tag="ACGI8A_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0179059.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKVKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            5919..6131
                     /locus_tag="ACGI8A_001053"
     CDS             5919..6131
                     /locus_tag="ACGI8A_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFH0179060.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            6332..6931
                     /locus_tag="ACGI8A_001054"
     CDS             6332..6931
                     /locus_tag="ACGI8A_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179061.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            7159..7530
                     /locus_tag="ACGI8A_001055"
     CDS             7159..7530
                     /locus_tag="ACGI8A_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0179062.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            7662..8936
                     /locus_tag="ACGI8A_001056"
     CDS             7662..8936
                     /locus_tag="ACGI8A_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFH0179063.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            9047..9991
                     /locus_tag="ACGI8A_001057"
     CDS             9047..9991
                     /locus_tag="ACGI8A_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179064.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            10207..11544
                     /gene="rsxC"
                     /locus_tag="ACGI8A_001058"
     CDS             10207..11544
                     /gene="rsxC"
                     /locus_tag="ACGI8A_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFH0179065.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            11567..12544
                     /locus_tag="ACGI8A_001059"
     CDS             11567..12544
                     /locus_tag="ACGI8A_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HFH0179066.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            12544..13113
                     /locus_tag="ACGI8A_001060"
     CDS             12544..13113
                     /locus_tag="ACGI8A_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HFH0179067.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            13126..13719
                     /locus_tag="ACGI8A_001061"
     CDS             13126..13719
                     /locus_tag="ACGI8A_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit E"
                     /protein_id="HFH0179068.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            13734..14309
                     /gene="rsxA"
                     /locus_tag="ACGI8A_001062"
     CDS             13734..14309
                     /gene="rsxA"
                     /locus_tag="ACGI8A_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFH0179069.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            14325..15296
                     /locus_tag="ACGI8A_001063"
     CDS             14325..15296
                     /locus_tag="ACGI8A_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFH0179070.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            15516..16100
                     /locus_tag="ACGI8A_001064"
     CDS             15516..16100
                     /locus_tag="ACGI8A_001064"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFH0179071.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            16145..16804
                     /gene="radC"
                     /locus_tag="ACGI8A_001065"
     CDS             16145..16804
                     /gene="radC"
                     /locus_tag="ACGI8A_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFH0179072.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            16874..17935
                     /locus_tag="ACGI8A_001066"
     CDS             16874..17935
                     /locus_tag="ACGI8A_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HFH0179073.1"
                     /translation="MAKEKKKEKKGFFSFNKMTKDMGIDLGTANTLVYIKGQGIVVRE
                     PSVVAIRDDSKEVLAVGEEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQK
                     AADKKGVVSPRIAICVPFGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVE
                     EPEGNMVVDIGGGTSEIAVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERT
                     AENVKINIGSTFKDDEEINMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIK
                     STLERTPPELASDIMENGIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTG
                     KSVEDQEIFEKVLMMNTKN"
     gene            17945..18838
                     /gene="mreC"
                     /locus_tag="ACGI8A_001067"
     CDS             17945..18838
                     /gene="mreC"
                     /locus_tag="ACGI8A_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFH0179074.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            18853..19341
                     /gene="mreD"
                     /locus_tag="ACGI8A_001068"
     CDS             18853..19341
                     /gene="mreD"
                     /locus_tag="ACGI8A_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFH0179075.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            19353..22331
                     /locus_tag="ACGI8A_001069"
     CDS             19353..22331
                     /locus_tag="ACGI8A_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFH0179076.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            22429..23112
                     /gene="minC"
                     /locus_tag="ACGI8A_001070"
     CDS             22429..23112
                     /gene="minC"
                     /locus_tag="ACGI8A_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFH0179077.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            23136..23933
                     /gene="minD"
                     /locus_tag="ACGI8A_001071"
     CDS             23136..23933
                     /gene="minD"
                     /locus_tag="ACGI8A_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFH0179078.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            23951..24235
                     /gene="minE"
                     /locus_tag="ACGI8A_001072"
     CDS             23951..24235
                     /gene="minE"
                     /locus_tag="ACGI8A_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFH0179079.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            24320..25450
                     /gene="rodA"
                     /locus_tag="ACGI8A_001073"
     CDS             24320..25450
                     /gene="rodA"
                     /locus_tag="ACGI8A_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HFH0179080.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            25491..25904
                     /locus_tag="ACGI8A_001074"
     CDS             25491..25904
                     /locus_tag="ACGI8A_001074"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFH0179081.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            26389..26718
                     /locus_tag="ACGI8A_001075"
     CDS             26389..26718
                     /locus_tag="ACGI8A_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFH0179082.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            26722..27513
                     /locus_tag="ACGI8A_001076"
     CDS             26722..27513
                     /locus_tag="ACGI8A_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFH0179083.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            27643..28463
                     /locus_tag="ACGI8A_001077"
                     /pseudo
     CDS             27643..28463
                     /locus_tag="ACGI8A_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428476.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(28675..29868)
                     /locus_tag="ACGI8A_001078"
     CDS             complement(28675..29868)
                     /locus_tag="ACGI8A_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HFH0179084.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            30187..32031
                     /locus_tag="ACGI8A_001079"
     CDS             30187..32031
                     /locus_tag="ACGI8A_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HFH0179085.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            32033..32734
                     /locus_tag="ACGI8A_001080"
     CDS             32033..32734
                     /locus_tag="ACGI8A_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HFH0179086.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGAESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            32752..34173
                     /locus_tag="ACGI8A_001081"
     CDS             32752..34173
                     /locus_tag="ACGI8A_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFH0179087.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            34274..34585
                     /gene="rplU"
                     /locus_tag="ACGI8A_001082"
     CDS             34274..34585
                     /gene="rplU"
                     /locus_tag="ACGI8A_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFH0179088.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            34600..34917
                     /locus_tag="ACGI8A_001083"
     CDS             34600..34917
                     /locus_tag="ACGI8A_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFH0179089.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            34931..35221
                     /gene="rpmA"
                     /locus_tag="ACGI8A_001084"
     CDS             34931..35221
                     /gene="rpmA"
                     /locus_tag="ACGI8A_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFH0179090.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            35332..36609
                     /gene="obgE"
                     /locus_tag="ACGI8A_001085"
     CDS             35332..36609
                     /gene="obgE"
                     /locus_tag="ACGI8A_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFH0179091.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            36661..37029
                     /locus_tag="ACGI8A_001086"
     CDS             36661..37029
                     /locus_tag="ACGI8A_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HFH0179092.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     regulatory      37121..37227
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            37330..38535
                     /locus_tag="ACGI8A_001087"
     CDS             37330..38535
                     /locus_tag="ACGI8A_001087"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFH0179093.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     gene            complement(38736..39302)
                     /locus_tag="ACGI8A_001088"
     CDS             complement(38736..39302)
                     /locus_tag="ACGI8A_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0179094.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
BASE COUNT        15482 a         4297 c         7491 g        12107 t
ORIGIN      
        1 agtaatttca aaaaataact taaaatttaa atcaaaaata aaaaaactcc taatctattg
       61 aataaatagc tttaggagtt tttaatggtg cccgaggccg gactcgaacc ggcacggagt
      121 attagtccgg tggattttga gtccactgcg tctaccaatt ccaccactcg ggcatgtcat
      181 gtatattatt atagcaaaaa aatatattgt ttgcaacata tttaatttta atatactatt
      241 attattatgg gaatattaaa taacagtata ttggtatata atatatatgt tacaaaaata
      301 gataattttt aggagtgata taatttggaa aaaaaattaa ttataataga tggaaattca
      361 ataataaata gagcttttta tgctcttcca gaaatgaata ataaagaagg gctaaagacc
      421 aatgcaatat atggatttac aacaatgcta tttaaaatga tagatatata taaaccaaca
      481 catataagtg tagcatttga cagaaaagca cctacattta gacatttaga atataaagag
      541 tataaggctg gaagaaaggg aatgccagat gaactggcag aacaattaca gccattaaaa
      601 gaccttttag ataaatttag aataaataga ttggaaatag atggctatga ggcagatgat
      661 attataggta ctgtttctaa aaaagcagaa gaaaatggat ataaggtata catagtaact
      721 ggagacaaag atgctattca acttgcttca gacaatacaa ctactcttat aacaaaaaaa
      781 ggagttggag aagttgaaga atacaacttt aattctgtaa ttgaaaaata cgagatgact
      841 ccaacacaat ttatcgattt aaagggtctt atgggagaca agtctgataa tatacctgga
      901 gtacctggaa taggtgaaaa aacaggtata aagcttataa aagagttttc tagtatagag
      961 aatctaatag agaatacaga taagcttaaa ggtagtgtaa aaaagaaaat agaggaaaac
     1021 aaagaaatag ctattcaaag taaaagactt gcaacaatta taagagatgt gccaatagaa
     1081 gtggatttag atagtatgat ttttggcgat tatgataaag atgaactttt agataaattt
     1141 agatactttg gatttacaag tctcttatca agattagttg atttggtcga ttcagatgac
     1201 acagaacacg tagaagaaaa aatagaaata ttaaaattaa aagatataga aaaatttata
     1261 gatgaagtaa acaaaaaagg acaagttatt ttaaaaactg ttagaggaca aggtaatata
     1321 cttgaaaaaa atataatata tatttttata agtgtagatg gagaaaaaat atattatgta
     1381 gattcaaatg aagtgtcaaa gttagatagt atattttcaa ataatgaaat taaaaagtat
     1441 ggatataatt taaaagatga ttatatatct ttaaaaccat ataatataaa cttagaaaat
     1501 atgtattttg atataacaat agctgagtac ttaatagatt caacttcatc tacctcatat
     1561 gattgtagtg caatagctat gaagtattta tcaaaaaagg ttaaatcaaa agaagaatta
     1621 ttaggaaagg gtgttaaatc taaaaactat gaggatttag aatttgagga tttagcagag
     1681 tatatgggtc aaataatatg cacagttaaa gaaacagttc ctatgatgga aagaagtcta
     1741 atggatatga gtatggatgg tcttttttat catgtagaga tgcctttagt agaagtctta
     1801 ggtcatatgg agtatgaagg tataaaagta gacaaaagta tattggatga attgagtgtt
     1861 gaatttaaag aaataatagc tacattagaa aaggaaattt atgaattatc aggagagcca
     1921 ttcaatataa attcaccaaa acaattgggt gttatattat ttgaaaaatt agagcttcca
     1981 ataattaaaa aaactaaaac tggatattca actaatgcag atgttttaga aaaattaaga
     2041 gataagcatc caataataga taagataact gagtatagac aaatagtaaa gttaaattct
     2101 acttacgttg aaggactttt aaatataata aatccagaaa gtaatagaat acattcatca
     2161 tttaatcaaa ctataacgac tacaggaaga atttcatcta cagaaccaaa tcttcaaaat
     2221 atacctataa aaatggaaat gggtagaaaa ataagaaaag tgtttattgc agatgataac
     2281 tgtaagttgg ttgatgccga ttactcacaa gtagagctaa gagttcttgc acatatgagt
     2341 caggatgaaa atatgataga agcatttaaa catcatgaag atattcatac caaaacagct
     2401 tcacaagttt ttaatgttcc tatggaagat gttactagtg aattgagagg tgcagctaaa
     2461 gctgtaaact ttggtattat atatggtaaa agtgactttg gtctagctga tgatttaaat
     2521 atacctgttc caaaggcgaa agaatatatc gaaagttatt ttgctaaata ccataaaata
     2581 aaagagttta tggacacaac agtagaaaag gctagtgagg atggatatgc tgtaacaatt
     2641 ttaaacagaa gaagatatat accagaaata aagtcaagca attttatgga aagaaataga
     2701 ggaaagagat ttgcaatgaa tgcaccaata caaggaagtg cagcagatat aataaaaatt
     2761 gctatgataa atgttcataa aaaattggaa gagaacaatt tgaaatcaaa acttatacta
     2821 caagttcacg acgagttgat agttgaagca attgatgatg aaatagatat agttaagaaa
     2881 atagtaaaag aagaaatgga aaatgctgta aatatggatg ttcacttaga tgtagattta
     2941 aatgtaggta gttcttggta tgagacaaag taggtgattt tatgttgata ttaggactta
     3001 caggaggaat aggctgtgga aaaagcagcc tatcaaatat atttagaaat cttaacattc
     3061 ctatagtgga tgctgatatt atatccagga agatatttga ggataagttg ttattggaaa
     3121 aagtatttgt ccattttggg caaagtataa aaaatgatga tggaactcta aatagaaaag
     3181 ctcttgggaa aatagttttt agtgatgaag aaaaacttaa ggaattaaat aacttgactc
     3241 atccacgaat aagagaaaaa ataataagtg aaatagaaaa attgagaaaa aaaggcgaaa
     3301 atatagtagt gttagatgcg gcaatattgg tagaaagtgg ttttttagat atggttgaca
     3361 agctattagt agttacttgt aaacaagaag tacaaattag tagaatacaa aagagagata
     3421 attgcagtga gcaggaagca cttagtagga taaattcaca gatgagtcag gaagaaaaat
     3481 ccaaatatgg tgattatata atagataatt cgggaacaat aactgaatta gaaagtaaag
     3541 cacataaatt tattgagtac atgaaggaga attggcgtga atagtaagaa agtattgatt
     3601 ctatctattt ttataatctt atttggggca ctattaatgg aaagcaaagt aatacataaa
     3661 tttttatatc ctaaaaaata ttcagagtat gtagaaaagt attcgaaaga atttaattta
     3721 gatgaaaata tagtttacag tgttattaaa gccgaaagta agtttaatag ttctgctgtt
     3781 tcaaaaaagg aagcaaaagg attaatgcaa atattagaca taactagaga ttggggagca
     3841 gaggaactaa atttaaaaaa tgtggatatt ttcgacccag agactaatat aagacttggc
     3901 tgttggtatt taagtaagtt atacaaagaa tttggtaaat tagatttagt gatagctgca
     3961 tataatggtg gttcaggtaa tgtgaaaaaa tggttagaaa ataatgaata tagtaaagat
     4021 ggcgaaaatc tacatgatat accttttaag caaacttcaa aatatgtaga aaaagtaaaa
     4081 aataattacg aacattataa taagatatat ggcaagaaag gaaaaaacta atgaagagaa
     4141 taaaagtctt aacagtcgtt ttagctataa ctcttatggt agctggttgt agtaatacta
     4201 aaacaaaaca aagtagtagt gattcaagtt tacaaagtca aagttctata gatgctaagt
     4261 atataaactt aacaatggta actccaaaaa ctataaatcc tataactaat actgataagt
     4321 ctgttggtta tataatgaat ttagtttatg atagtttatt tactatagat gagaactaca
     4381 atgtaattcc tcaattagtt aaggagtata atatagctca ggatggaatg agtatagata
     4441 taaagctaaa agatgcaaag tggcatgatg gaaaaaatgt tacttcaaat gatgtaaact
     4501 atactattgg tttaattcaa aaaagtgcag atagtccata taatgaattt actaaaaata
     4561 tagcttctgt gaatattaaa tcagataaag attttacaat aaaatttaaa gctagatatg
     4621 ctttttcaat tgatagttta atttttccaa ttgtatctca aaatcattta gattcaaaag
     4681 atgtaaatga caagaataat aatatgatag gaaatgggaa atataaaata gaatcatata
     4741 cagaaagaga aggaatggtt ttatctgtaa acaaagatta ctatgaagaa gttccaaaaa
     4801 ccatgaagaa cataaaagta ggtatggtgc ctaatgaaga tgcaaggaca tctatggtta
     4861 tggctcttga tagtgatata accaatgtta cattaaatga tttaagtaag tttcaagaaa
     4921 aagagtttaa tataacaaaa tatcaaggaa gagattatga gtgtgtatta tttaattata
     4981 ataatccttt tttcaaagat gttaatttta gaaaagcaat tgcacactct atagataaag
     5041 atagaattat tagtgaaggt tatatggatg atgctactcc agttaatttt ccacttaatt
     5101 caaaatcaaa atactataat agtgaaatga aggatttaga gtttaataaa gataaggcga
     5161 tagagtgtct tgctaaggta gaatatgcaa atgtaaactc agtaaatcaa aatgacaata
     5221 aagtaaaaaa tcagaaaaaa tcagctaaaa agaactcaaa gaagcttacg ccagaagaag
     5281 aagcaaaagt taaaaaagca gaagaagata gaatcaaaaa agaagctgaa gagaaaaaaa
     5341 atagagaaaa agaggaagta aagaaaagtc tttctgaaat gaacttaaag ataatagtaa
     5401 acagagataa tagtgagaga ataaaaactg caaacataat aaatgaaaac ttaaaagcta
     5461 taggaataaa tagtacagta aatcagctct cagataagga tatggaaaat gctcttaatt
     5521 ctaaaaatta tgatttagca ttagttggat ggaaactatc aattatacca gatgcaagta
     5581 gtattatagc gagtagtgga tatactgatg ataagttaaa tggatatatg tctgcactta
     5641 caagttctac gtctgaaatt gaaacaaaaa aagcatacaa agatgtacag acatatataa
     5701 aagataatgc aacatttata agtttagctg tgagaaacaa ctatattgta agtaatagta
     5761 gattaaaagg taaaattact ccaaatgatt ttgatgtata tgagggaata tctaatttag
     5821 atataaaatc gaatgaatca aattaaatga ttaatttcta tttaaaaagt ataaactata
     5881 tgatagaata cccttatagg gtataaggag gtaattaaat gaagaagaaa ctattagtag
     5941 aaggtatgag ttgtggtcat tgtgtaaacc atcttaaaac tgcattaaca gaagatatag
     6001 atggtattga agttttagat gtagatttag aaaataagtg tgcatctgta gatatgaaag
     6061 atgatgtatc tattgaaaaa ttaaaagaag tgatagcaga attaggattt gaactaaagg
     6121 gaattgaata gaaattgata aactaaaaaa agacctctat tgggggagta atagaggtct
     6181 aaaaatgggg gagattgagt atgtttgatt ttacattact attatctccc cttttaaatt
     6241 aatatataca taaaaagtaa aaaaattttg taatgactca catttcttga gctttttgaa
     6301 tatactataa atagctaagg gagggagtat catggaatct aggggatata aagaggattt
     6361 aaaaaaaatg atacttgata tggcccacaa agaacttgag agttatgtat cacaaaaagg
     6421 agcaaaaaaa tactttgaag gtaacttgga taagattatt aataataata taaaaaaatt
     6481 caatcaaagt attggtctta atagagtata tttaaaaatg ttaaaaaagt gtgcatatca
     6541 agaaagtgta acttcaatat caagaactat aaagaaagaa gcattattta aatcaaaaaa
     6601 agagctattt gctttttcag agtacttaaa acttgatatt aacaagcgac tttcatataa
     6661 tcaaatttta agaaaaatat caagatatat atactacaat agaagttcat atgctcaaaa
     6721 atacgttgtt ttcaaaagag gagatgaaga atatctttta gaaccagaga aaataaaaga
     6781 tgaattaata tcaagctata ggtcaaagac tagagaagat atgaggtcaa tagcaaaact
     6841 tttggatata gaagtagaag atgattataa tgctgaagat attagaaaaa aagtaataaa
     6901 ttatattatt aaagaaaaac ttaaaagtta gagttttaaa gaataagttc tgccaatttg
     6961 catgattaag tataacttgg gtataaattt tttatgcatt tgaaagagct tattcttttt
     7021 ttgacatatt cttttaaata atttaccatg atttggtaat tggtcatcat cattcaaatt
     7081 tttaacttaa tatgatttaa ataaatttat aataatgata taatataaaa aaaataaaaa
     7141 ttggagagtg atttcaaaat gaagtttaat ttttgtcata acaatttcaa tgttactaat
     7201 ttagaaaaaa gtttagattt ttataaaaaa gctttagggc ttaaagaggt aaagcgcaag
     7261 gaagcagaag atggaagttt tatattagtt tatttaggag atggcattac tagtcataca
     7321 ttagaattaa cttggcttag agattgggat agaccatata atttaggaga caatgaattt
     7381 catctggctt tagaagttga ggagtttgat gaggcaaaaa aacttcataa agatttagat
     7441 tgcatatgtt ttgaaaatga aagtatggga atttatttta tagcggaccc agataattat
     7501 tggattgaaa tacttcctaa aaatcactaa ttttaaggat atgtttataa tttctttaca
     7561 attaataaga aaataagtta tcattaaatt gtagggacta aaaatggata ataatgttat
     7621 gaatttatga ctataaaaat ttgttggggg gagtgatagt agtgaaaaaa gctatagcag
     7681 ctttaggaat tggggcagta gcagtatctg ttagctccat aaatgcaagt gctcttgaaa
     7741 agggaacagt aacagcaagt gccttgaata taagaagtgg accaagttct gattgcgata
     7801 aggtagcaaa attatacaag ggaaaaactg tagagatttt agaaaagtct aatggatggt
     7861 ataaagtaag ggtatcgagt tctgttgttg gatggggaag tgcaaaatac atatcaacaa
     7921 gtggttcgtc tgaaggtaca tcaagtcaaa ataatccaac ttcaagtggg acaactatta
     7981 gtggaaatgg taaagtaaat gtgagttcta gactgaatgt aagaagtggt gcaggtacta
     8041 actattcatt agttggtaaa gcaaataatg gagatgtagt aaagttacta gaacaaagca
     8101 atggatggta taaaataaaa ctatcaaatg gagttactgg atgggctagt agccaatata
     8161 tttctaaaac atcagaagat gttggaacga acaattctag taattcaaat agtactaata
     8221 attctgataa aaaaccatct agcgaagaat caattgaagg caagaatggt aaggtaacat
     8281 ctgctgtgag tttaaatgta agaagtggtc ctgggacaag ctattctata ataggaaaat
     8341 taaatggtgg agatgttgtt gaattaaaag ctaaaagtaa tggttggtat aaggtaaagt
     8401 tatcgagtgg aacaattgga tgggtgagtg ccagttatat ttctgagact aatgaggata
     8461 caaaagaaaa accaaattca tcatctaacc aaaattctca atctaatagt aactcaaagc
     8521 catcatttac tggaaattca gataaatcta cagcaaaagg ttcaacaatc gtagattttg
     8581 catatactct tattggaata ccatatcaat ggggagcttc aggacctgat aaatttgact
     8641 gttctggatt tacacagtat gtttttaaac attctgtagg cgtttctatt cctagagttt
     8701 ctagagaaca ggctaatttt ggaagtgcaa tttctatggg aaattatgca ccaggagatt
     8761 tagtttattt tgacactgat ggtgatggaa caactaatca tgtaggtata tacgtaggaa
     8821 atagtaaatt tatccattgt agtggtactc agactaatcc taataaggtt aaagtggata
     8881 atttaactag ttcatattgg tctaaagtat tacttggtgc aagaagattt gtttagtaag
     8941 tacaatttct tattaaaaaa ttagaagaaa taattattta tattcataaa ttgtgactct
     9001 taaaaatata ctttattaat aatataattt tgggagggac aaactaatga atagaagaaa
     9061 aacgatatac agaggattta ataataatag acataagatt gatttacgta aatatgttat
     9121 aacgattgct tgcttgtgtc taattggcta ttattcttat acaaaaataa aagatagcaa
     9181 gatattggag tatgtatcgg ctaaaatacc atttctaaat aattcatcag atataactta
     9241 taaagatata tctgatgaat taaattcaat aaaaaaaggg aaaaaatcta aatcacaaac
     9301 caattcagat gataaacaag aaactaatcc agaaaaagcg gtaaataaca ctaaagagcc
     9361 agaagaagtc aaattagcta ctatagaagg ctgggatatg tatactattc aagttgctgc
     9421 aatagataat aatgatgatt tgaaaaaaat acagacatca ttagttaata atgacatacc
     9481 tttttcagtt atggagaagg atggagtaaa aaaaatacag acctattcct cttttgatga
     9541 aaatgatgtt agaaagcaga taagcagtgt aagaaaggtg tttccagatg catttttatc
     9601 acacttagat gcacctatgt tgtctttaga gtatacaagt aactacgctt atatagagag
     9661 tatatctaaa gaattaaata aattaataac aaactttaaa gaagaatcaa gtttttggtc
     9721 gaatgctgaa aataatgtag atatggaaaa atataatact attttgacaa atagaaaggc
     9781 aatatcacaa aatatatcaa aagaagctga aaagattgat tattctgaaa tgcgtttatt
     9841 taaagataat ttaatagagt atgtaaagaa tgtgaatgaa aaaattgata cagcttcaaa
     9901 agcagcaaat gaagaaaaat atagtgtaag taaaagtctg cttttatcat ccatgcagga
     9961 gtactctatg tttataaatt ctataaaata aaatacagaa aatttggact ttagaaagct
    10021 atatgaaaag gattgtttaa ttaagcaatc cttttcctca atttgactta atatagaggt
    10081 gttgatttat aaaataaatt ataaaaatac ggatattttt ttaacaattg ttaagatttt
    10141 ttaatatata tagtatactt atagaaggca gaaaaaatgt ttttttatag ataagggggt
    10201 aaaacaatga aactcttaac ttttaaggga gggatacatc ctccatatag aaaagagtac
    10261 tctaacacaa aggcacttga aaaagctcaa gctccaaaga tagtttacat tcctcttcag
    10321 caacatatag gggcaccagc taaacctatt gttgaagttg gtgatgaagt aaagtttggg
    10381 caaaaaatag gtgaacaaca aggttttgtt tcatgtaacg ttcattcttc agtatcaggt
    10441 aaagttattg ctattgaaca acatgaagta ccaggaggtt cagctcaatg tgtagttatc
    10501 gaaaatgact ttaaggaaga attacatgag agtgttcaac caaaaggtca gttagaggat
    10561 ttgagcaaag aagatatagt aggtatcata aaagaggcag gaatagtagg tatgggtgga
    10621 gctacattcc ctaaccatgt aaaagtatct cctccaccag atagcaaggc agaagtagtt
    10681 atattaaatg gtgcagaatg tgaaccatac ttaactgcag accacagatt aatggttgaa
    10741 aatccagaag atgttgtgtt tggtttaaga gcattaatga aggtattaga tgtcaaaaaa
    10801 ggatttatag gaattgaaac gaataaacca gatgctatag aagcaattca aaatgtagct
    10861 aaagactata gcgaaatcga agttgttggc cttcaagtga aatatccaca aggagcagag
    10921 aaacaactta tttatgcgtg tacaggcaaa gaagttccat ctggtggatt accaatagca
    10981 gcaggtgcag ttgttgacaa cgttgccaca gcagcacaga tagctaagtc tattaaaaca
    11041 ggtatgcctt tggttgaaag aatcactaca ataacaggta gttgcatcaa agagccaaag
    11101 aacctaataa caaaagttgg aacattagtt tcagaaataa tagaacaatg tggtggattt
    11161 aaagaagata agaagattgg taaggttata atgggtggac ctatgatggg aatagctcaa
    11221 tatactactg aaatagcgac taacaaaggt tcttcaggaa ttttatgttt ggatgaggaa
    11281 gaatcacgta ctccagatat tcaaaactgt ttaagatgtg gaagatgtac agatgtatgt
    11341 ccatcattct tacaaccact tttcataagt gcatattctt taaaagatga ctatgacaca
    11401 gctgaatatc atagagcaat ggattgtata gaatgtgggt cttgctcatt tatttgtcca
    11461 gcaagaagac cattacttca atctataaga tcagcaaaga gagaaatagg agcaaagaga
    11521 agaaagcaag ctgctcagaa ataaagaaag ttaaggggga aaaacgatgg aaaataagtt
    11581 gatagtatca tcttctcctc atgtgagaag taatgaagat acttcatata taatgaaaca
    11641 agttattata gcactccttc cagcagcagt agcaggagta tacttcttta gacttaatgc
    11701 tttgagtgct atgttttttt gtatacttgg tacagtaggt actgaatttt tatatcaaaa
    11761 atttacgaag caaaaaagta ctataggaga tttttcagct gttgtaacag gattattatt
    11821 agcatttaac gtaccagcat cacttccttg gtggatgtgt ctagtaggag gaatatttgc
    11881 aatattagta gttaaaatgg tatttggtgg aattggatgt aactttgtca atccagcact
    11941 tgctgcaaga gcatttttat tagcatcatt cccagtagca atgactgctt ggactcaacc
    12001 aggtgttaac tggataggta aaaatttaga tgcagttact acagcaactc cattaagctt
    12061 tttgaaaaat ggagcagcag gactggcaga cctttctagc aatggaatta gtcttgctga
    12121 catgatgata gggaatattg gtggatgtat aggtgagaca tcagcaatat tattattatt
    12181 aggtggagta tacctaatgt ataaaggcat aataaattat gttataccag tattttacat
    12241 agcaactgta tttatattaa cattcctttt aggtggattt aatataactt tcgcaatata
    12301 tcaactgttt gctggaggac ttatgttagg tggattcttc atgcttacgg attatacaac
    12361 ttcgcctatg actaaaaaag gtcaaataat atacgctgta ttagctggtc ttataacaac
    12421 tgttataaga atgtatggtg gatatccaga aggcgtatct tactcaatac tattagtgaa
    12481 ctgtcttgct ccacttatag ataagtttgt tagaaataga gtatttgggg aggtggctaa
    12541 ataatgaaca gtatggtgag attaggtggt actttacttg ctataagtgc aatagctgca
    12601 ttagctcttg gagctactaa tcaggttaca gctccagtaa tagaacaaag aaatatacaa
    12661 gcaaataatg aacttagaaa agcagtcctt ccagaagcaa aagaatttaa agaaatgaag
    12721 gaagatactt acaaaggcct tggagatggt cttatagctg aagtgtatga aggtttagat
    12781 ggttctgaag tggttggata tactttaaaa gctaaaccaa gtggatatgg tggagaaatt
    12841 gaagttatgg taggtatatc atctgaaggt caagttacag gtgttgacat tggtaatatg
    12901 tcagaaacag caggtcttgg agcaaaagct aaggatgatg catttaaggg tcaatacaaa
    12961 ggaaaaactg ctgagcctct tgaagtagca aaaggaagta caacagcaga taatcaaata
    13021 ttagcaatat caggtgcaac tattacatct acagcagtta ctacaggagt aaatgctgct
    13081 atagatgttt tcaatagtgc tcttaataaa taaggagggt taattatgaa tttagctaaa
    13141 gtatttaaaa atgggctaat agatgagaac ccaacatttg ttcaagttat aggtatgtgt
    13201 ccaacactag ccgttacaac ttcagcaata aatggaattg gtatgggtct ttcaacagca
    13261 gcagttttaa tatgtgcaaa tttagttata tctttaataa gaaaaattac accagataag
    13321 ataagaatac ctatatttat agtagttata gctacatttg ttacaatagt aggtatgctt
    13381 ttaaaagcat atgttcctgc tcttgataag gctcttggta tatatatacc attaatagtt
    13441 gttaactgtt taatattagc tcgtgcagaa agttttgctt tcaaaacagg agctatgcct
    13501 tcaatagttg atggtgtagg tcaaggtctt ggatttactg ttgcacttac aataataggt
    13561 gcagtaagag aattacttgg aaatggtagt ttatttggaa tgacattatt tggagcatct
    13621 ttccaaccag tattaatatt tatattacca ccaggtgcat tcttaacatt aggattctta
    13681 tttgcaggat ttaataaatt aagaagtaaa aaagcatagt ggaggtgtaa taaatgaatt
    13741 taatactttt atttttaagt atcgttcttg ttaataacgt tataacatct caattcttgg
    13801 gtatatgtcc tttcttggga gtttctaaga aggttgatac agcagtaggg atgggagttg
    13861 cagttacgtt cgttttaact ctagcttcaa ttataactta ttttatacag attttattag
    13921 taaaaactgg aactggattc ttacaaacaa tagcttttat attggttata gcttctatag
    13981 ttcagttcgt tgaaatggta attcaaaaga tgagtccatc tttatatcaa gcattaggag
    14041 tttatttacc tcttataact acaaactgtg cagttcttgg tatagctcta gttaatgttc
    14101 aaaaaagtta taatttagta gaaacaataa taaacggatt tggagccgga gctggattta
    14161 ctttagcaat agtaatattc gcaggtataa gagaaagact agaattagct gatataccag
    14221 aagcatttaa aggtttccct ataacgctta tatcagcagg tttaatgtca atagctttct
    14281 taggatttac aggacttata aagctataaa aaagaggggt gaaaatggtg atacttacag
    14341 ctgtattagt tttaggaatt atgggcttaa tcttcgggat agtactagat tttgcatcta
    14401 aaaagtttgc tgtagaagta gacgagagag tggaagctat actaggagta cttccaggag
    14461 ccaactgtgg aggttgtgga tttccaggtt gtggaggtct tgcaaatgcg atagttgaag
    14521 gcaatgcacc agttaatggt tgccctgtag gtggagcaga tgttggtgca aaagttggag
    14581 aaataatggg aataagtgct gaagcaggtg agaaacaagt agcaaaagtt atttgtaaag
    14641 gaacttgttc aagtgcaaaa gataaatatg agtatgaagg aatatcagat tgtagagcag
    14701 caaatgtttt aaactcaggt gctaagatgt gtaaatttgg atgtttaggt cttggaacat
    14761 gtaaagatgc ttgtaaattt gatgctattt ctatagttga tggaatagca gtaatagatg
    14821 aagaaaagtg tgtaaattgt ggaaaatgta aagaagtttg tcctaagggt ataataatca
    14881 caaaaccaga aagtcaagaa gtagttgtag agtgtaatag caaagagttt ggtaaagctg
    14941 ttaaagaaaa atgtactgct ggatgtattg gctgtggaat gtgtgtaaaa gcttgtaaat
    15001 ttgatgcaat aatatttgaa gacaaaatag ctaagataga ccctaataag tgtgtaggtt
    15061 gtatgcaatg tgtagcaaaa tgccctacaa aagtaatatc aggtgacata actaagaaaa
    15121 agaaagttac tattgaccaa gaactttgtg ttggatgtac agtgtgcaaa aaacaatgta
    15181 agtttgatgc aatagaggga gaattaaaag aaaaacataa ggtagatgca gataaatgtg
    15241 taggatgcca tttatgtatg gaaaaatgtc caaagaaagc tattaaaatt ttgtaatatg
    15301 gacaaaataa ggatatggaa taatttccat atctctaatg agtacataaa acagaggtat
    15361 ggaataattt ccatacctct attttatgta tatttattct aatggtaata aatataacaa
    15421 tagtgtaaca aaaaaggttc gacatttctg ttgaattttt tacaatatag tgatagacta
    15481 aataggtaca aattacgtac aaatgaggtg aataaatgaa tataatcttg gcatcagcct
    15541 ctccaagaag gaaagaaatt ctagagaata cgaatgtaag atttgatata attaaaaatg
    15601 aaattgatga gattatattg gaaggtgaaa caccaaaaca tttagttatg agattagctt
    15661 ttgaaaaaag tatgtctgta gcatctgaac ataatgaaga tgttgttatt ggagctgata
    15721 cagttgttgt tttagacaat gctattttag gaaaaccaaa agatgagtct tgtgcaagag
    15781 atatgttaaa aagactatct ggaagagagc atcaagtcat aacaggtatt agcttaataa
    15841 acttgtgcga agataaaaag gtaattgact atgtaatcag caatgtgaaa tttaaaacgt
    15901 tatcagagca ggatattgag gattatctga aaactaatga gtcatttgac aaagcaggag
    15961 cctatggtat acaaggttat ggagctttgt tagttgaaga aattagagga gattatttta
    16021 atatagttgg tcttccaatt tcaaggttag gcgacctatt aaaaaaatat tttagtataa
    16081 atctttttta tggagtgtga tttatctgaa aaagtcattt aattcaacca ttaaggtaaa
    16141 agaaatggca ccagaggaaa gacccagaga aaagatgctt gccaaaggcg taaaaagctt
    16201 atcaaatgct gagttgttgg ctatactttt aagaactggc aacaagaata aaaatgctat
    16261 tgaattagcc aactatataa taaatagaga tattcagggt attagacatt tagaagatat
    16321 gacaatagag gagctgtgta atatagatgg aattgggtta tcaaaatcaa cccaaattaa
    16381 agcagcttta gaactaggct ctagagtagc tagttttaaa cctataaagt ataaaataag
    16441 gaatccttgg gatatacaga ggtattatat ggatagccta aggtatttaa aaaaagaggt
    16501 ttttaaagcg gttcttttaa acacaaaaaa tgagataata tctgatgtag atgtatctat
    16561 agggacttta agctcgtcat tagtccatcc aagagaggtt tttaaagaag ctataagaag
    16621 aagtgcaagt aagataatag tgatgcataa tcacccttca ggaagtgtag aaccatcaag
    16681 agaagataaa aatatcacat caagacttat taaatgtgga gaaataattg gaatagaaat
    16741 aatagaccat ataattattg gagatggatt atactttagc tttaaagaaa atatgataat
    16801 ttgactttat atgtgataaa ataatagaag aatgttttaa acatgattct taatagtagg
    16861 aaggagtaaa atcatggcta aggaaaaaaa gaaagaaaaa aaaggttttt ttagttttaa
    16921 taagatgaca aaggatatgg gaatagattt agggactgca aatacattag tttacataaa
    16981 aggacaaggt attgttgtta gagaaccatc agttgtcgca ataagagatg atagtaaaga
    17041 ggtattagca gtaggtgaag aagccaaaaa aatgataggt agaacaccag gaaatatagt
    17101 agctataaga cctatgaaag atggagtaat agctgatttt gacataactc aatcaatgat
    17161 tagctatttt atacaaaaag cggctgataa aaaaggtgtt gtaagtccaa gaatagctat
    17221 atgtgttcct tttggagtta cagaagtaga aaagagagct atagaagagg ctgcaagaca
    17281 agcaggagct aaagatgcat tccttataga agagccaatg gcagcggcta taggggctgg
    17341 attaaaagtt gaagaaccag aaggtaatat ggtagttgat attggtggag gtacttcaga
    17401 aatagctgta atatctttag gaggaatcgt tactgctaaa tctataagaa taggtggaga
    17461 cgaatttgac gagtctatag tggcttatgt aaaaaaagaa tataatctta tgataggtga
    17521 aagaactgct gaaaatgtaa aaattaacat aggttctaca tttaaagatg atgaagaaat
    17581 aaatatgcaa ataagaggta gagatttaat ctcaggatta cctaaaacaa tagaaatatg
    17641 ctctacagaa gttagagaag cattaaaaga acctgtaagt tcaatagtag atgcaataaa
    17701 atctacacta gaaagaactc caccagaatt agcctcagat ataatggaaa acggaataat
    17761 gctaacagga ggaggcgctt tgcttagagg tcttgataag cttattacac aggaaactgg
    17821 tatgtctgta caaatagcag aaacacctct tgattgtgtt gcacttggaa caggtaaatc
    17881 tgttgaagac caagaaatct ttgaaaaagt attaatgatg aatactaaga attagaagtt
    17941 ggtgatggcc ttgagatttg acaagaacaa aaatgagaaa aaaaggatta atgttaaagt
    18001 gatagcaacg ggcgtagttg ctatcacttt aattggaatt gtgggtatat caattggtaa
    18061 attttctagt ggtagtccag taaatttagg agtagcttca gatgctatta caagtgtagg
    18121 taagggaata aatgatggat tttcttttat caaaaatggt tttaaagatg tagcaaattt
    18181 taaagataat tctaaaaagg taaaaaaatt agaagaagaa aatgaaaaac ttaaaaaaaa
    18241 tatgatagct ttaaatgcca aactagataa aacagaatcg ctagaagagt taaaaaagac
    18301 actgaacttt gtacaagaag agtataaggc gacaagtatt tcagcaagtg ttgttggtaa
    18361 aaatgatgga aactggtatg agagttttgt cataggtgca ggaaaaaatt caggtgtaaa
    18421 aaaagaaagt atagttatga atggtagtgg attagttggc atagtttatg aagtttccaa
    18481 caattatagt aaggcaattt cactattgga ttctaaggca tctgtaagct ttaaactagc
    18541 aaaggatgcc aatgctaaag gtacaattac tcaaaatact actttagata ataaagatag
    18601 ttataatagt aaaggatatt tgcaaggata tatgtttgac tcatcatata atgtaattca
    18661 aggagatata ataacaacat caggtcttgg attcttccca gatggtatac caattggcga
    18721 agttgaaaaa gtggttgatg ataaagataa gtcattaaaa tatgtagttg taaaacctta
    18781 tgttgatttt aagaatataa atgacgttgt agtcatagaa ccaagaaata taggataaag
    18841 gggataaaaa caatgaaaaa agttttactt tgtctgttgg ggattgtgct tatgactata
    18901 gaaaatagta taacaaatta tatagatatt tttggcataa gctttaattt agttcttata
    18961 tatgtaacca taatctctct ttatttagat gaattagaag gtgggattat agcagcaata
    19021 atagggctta ttaaagacat aaccgttggt ggaatttttg gtgtaaatgc attaattttg
    19081 tttattattg catatgcaat tggatacatg agagatcagc tatataaagg tagttatatt
    19141 attactttcg ttttaatttt tataggcagt ttaattgatt caattgtcaa tataggtaca
    19201 tctagtataa tttatcaaag ctacaacata tcaacacttt ttgtaaaagg tctactagta
    19261 gcaccaatag tgaatagttt aattggttta gtaatatatc gcttatctaa aagagcagtt
    19321 ttaaaactta aagaagatta gttggtgatt tcatggaaga aaataaacaa gttgatagac
    19381 taaaaataat taagatagtc atgggcgtga tatttttagc tattttagta aaaataatct
    19441 atatgactac atttaaatat gaatattaca atgagttagc tgagaataaa acatataaaa
    19501 agttggcaat agaagctcca agaggagaaa taaaagatag atatggaaga ctgttggcag
    19561 gaaataaaaa cttatttact gttcaagttt caggtaacga tattaataag aaagatgcaa
    19621 ataagcattc aagagcaaat gaaatatcat taaaacttat aaatttgctg gagagaaatg
    19681 gtgaagaata tgtagatgag tttcctatat atgtagaaaa tggaaagtac tattatacat
    19741 atgacagaga tattagggaa tataaatcag aaaatggtat tccaaatgat tataatgcta
    19801 aggaaagttt ttattacttg gtggacaaac ttatatcagc tggaatatta tctcaagaag
    19861 ataaaagatt ggatgcgaca agattacagg caaaactaaa tgaaaatgga tattatccac
    19921 ctattttagt tagtaaatgg atgttcacag cagaaagaga taagagagat tggttggcta
    19981 gttataagat aaaagaaact aaactgagtg ccaaagaagc gtttgaaaaa gtaagaaata
    20041 gtgatgcatt agaaattgat aaaagtttga gtgatgaaga tgcaagaaaa ataatggtcg
    20101 taagggattt gataaaatca aaaggatatt ctcaatataa tccagtaact attgctaagg
    20161 atgtaggaga aactacgatt gctcaaattg aagaaagtgc tatggacctt gttggtgttt
    20221 caatagcagt agaaccagta agatattatc ctaatgggtc attagcttct catatgttag
    20281 gatatgtagg aaaaatgcca tccactcaaa tagaatcata ccttcaaaaa ggttatgaaa
    20341 ctggagatat ggtaggtctt gcaggagtag aaaagagtaa tgaaagtaga ttaagaggaa
    20401 ctgatggtta taaaatggtt aaagttgatg cattgggtag aatcagtaaa gaaattgaat
    20461 ctaaaaaacc taaatcagga gatactgtgt atcttacact agataaagat ttacaagaag
    20521 tttcagacaa tgcattaaaa cagataatcg aagttgcttc taaaggaggt acttttaaaa
    20581 gtaaatttgg ggacaaacca attagtgcat atgcaggaaa agcacagtca gcagctctta
    20641 tagctattga tgttaaaaat ggagaagtat tagcttcatc aagttatcct aattatgacc
    20701 caaataaatt tgcaaaaggt atttctacag aggattataa agcacttcaa ccaaaaaatc
    20761 caaatgactt actagcagga agtccacttt taaacttggt tacacaaggg gaatttcagc
    20821 caggttcttc atttaagatg gtaacttcaa tggcagcgtt agaaaatggt ctggatccaa
    20881 actttactat aaatgaccct ggggttataa tgttaggtaa aaaatctttt ggtgactatg
    20941 tatggaacca tggtagagga aaccatggta tgacaaactt atataaagca atacaagagt
    21001 cttgtaatat atatatggca acaattggta caggtaaaac atggccagat ggaaaaagca
    21061 ttggtataga tatgaatgca aataaaatac ttgagtatgc aaaattattt gggcttgacc
    21121 aaaatacagg gcttcaagat gaagtagaag aaagagcagg taaagttcct agcacagaag
    21181 ataaattgaa atcaacacaa gctcttctaa agtcaaattt agaaagagaa atggcaaatg
    21241 attttgttga tataactaga gaaaaaaatc ctaaagaata tgagaaaaga atcaatgaga
    21301 ttgtaagctg ggcagcagaa aagaaaactc ctggtagagt tgaaacaatg aataggctaa
    21361 aaaaattgga tgttaaagaa gatagaattg aagaagtagc agatttagca gtatttagtt
    21421 attttaactt tgcaaaatgg agtactgcag atacatttaa cttagctata ggtcaaggtg
    21481 aaaatgctta tacaccagct caaatatcaa gatatgtagc tgctatagca aatggaggta
    21541 atttagtaga attatctgta gttgatagag ctgtttcaag tgattattca tcagttaaaa
    21601 ttaatgacca gaagaaagtc gaaaaaatac cttttaaaaa tccagataac ttaaaagaat
    21661 taacaaaagg tatgaaattg gttgcaagac aaggtacagc gaagagtgct tttgctgatt
    21721 ttccaataga tgtagcagca aaaactggta cagctgagaa aagtgggaaa atacctacag
    21781 ataacgagta tgaatattta aaaagtcata tgtcatctta taatgtcaac ctaaatgatg
    21841 cgattaaact agctgataag atgaaagcag aaaaagaaaa agagctttca ttagctaagg
    21901 aaaaggaaat aaaaaagaaa ttagaaaata aagatttgaa agacgaagaa agaaagaaat
    21961 tagaggaaga attagaagat ggagtaaaag ttagattaga ggacactgat aaggtgaatt
    22021 cttcatatct aagaaaagct ataaaagaat taaatccaaa aataacagat gaccaaatag
    22081 atagatttaa acaagattat ggttctttta cgtggactgt tgcttttgct ccagcagatg
    22141 acccagaaat agctgttgta tgtgtaattc ctcaaggtga ttcaagtgta ttctcacttt
    22201 taccaactag agaagttatt ggaacttata tgggattaga gccaactaat agtaaaaatg
    22261 acaacaagac gaatgatgtt aacaattctt ctgacgaaaa tataaatttc gagtcacaaa
    22321 ttaatagata gctcaatcta tgtataaact ttagctttat atagaaaaaa taaaagggtt
    22381 taaagcgtat ttgaagaata taaatgatat atcactctgg aggttgttat gtctttaaga
    22441 gaaatttgta gtcaagaact agtagagttc aagggtaata aaagagggat aatagtaaat
    22501 ataaaaagag aagctccatt tgaggaaata caggaaaaaa taataaacaa attggaagca
    22561 tatgttggct tttttaatgg ggctaaaatt agtaaaatta atagtgattg tttaactgac
    22621 atggagattc tagaacttaa agaaggaatt acttctagat ttgatgtgga atttgttgaa
    22681 gatcaaaaaa ttgaagaaaa tagtaatttt ccaacaaaat atgtcaatac cctaagatct
    22741 ggtgaaaata ttgaatttga aggtgatgta gttatattga atgatatgaa acctggttca
    22801 aaagtccttt caaaatcaaa tacagttgtt atgggagata taaatgctgg tgctaaggtt
    22861 gtagcaggag gtaatgtttt tgtaatgggt aaaatagaag gttttgtaca tgcaggtgca
    22921 gaaggcaatg aatttgcata tgttgttgct ggaaatctta atcctaaaat attacaaata
    22981 gcagataata ttgcagaagc tccagatgat gaagaaaatt atgaaagtga atcagaaatt
    23041 agcccagaaa tagcctttgt aagtaatgga agaattgtaa tcgaaagcta tttgtcaaag
    23101 ttagacaaat agaaagaaaa atagggggta gtattatgag cgaagttata gttataacat
    23161 ctggtaaagg tggggttgga aaaactacta ccgcagcaaa cttaggaact gcgctaagcc
    23221 tagaaaataa aaaaacagta gttgtggatg cagatattgg acttagaaac ttagatgtag
    23281 taatgggtct tgaaaatcga atagtttatg acatagtaga tgtagttgaa ggaacttgta
    23341 ggcttaagca agctctaata aaagacaaaa gatttgataa tctatattta ttaccagcag
    23401 cacaaactag agataaaaat gctgtctcag ttgagcagat gattgacctg tgtgagaaac
    23461 taaaagaatc tttcgagtat ataataatag attgtccagc aggtattgag caaggcttta
    23521 aaaatgcagt agctggggca gatagagcta ttgtagttac taatccagaa atatcagcag
    23581 taagagatgc tgatagaata ataggtctat tagaagcaaa tgaaataaaa gagataagat
    23641 tagttataaa tagaattaga aatgatatgg ttaagcgtgg agacatgatg gataaacaag
    23701 atataataga aatattagca atagatttgt taggtcttgt tcctgatgat gaaagcataa
    23761 ttatatcaac aaataaagga gagccagcta tacttgattc taagtcactt gctggtcaag
    23821 catacaaaaa tatcgcaaaa aggatactaa atgaagaagt tcctctactt gatcttgaag
    23881 ttgaagatgg attctttggt agacttaaaa aaatgtttag catggctaag tagcaaagga
    23941 ggatacatct gtgttagatt tatttagagt tttttctaac gaggctaaaa ctagtaaatc
    24001 tgttgctaaa gagaggttaa agctagtttt agttcatgat agagtagatt gttcaccaca
    24061 acttttagag atgataaaaa cagacatttt gaaagtaata gctaattatg cagaaataga
    24121 agatgatggt cttgaaataa aaatgtctaa atgcagaggt gagcatgatg acaaacctgt
    24181 atctgcatta gttgctaata taccactaaa gaatataaaa gatagatgta tgtaattagt
    24241 gcttaaaagg gggatatcct tattggtata tgcccctttt atttaattat atgtttaaag
    24301 atagaaagac ggtgattctt tgaaaaaaat caatattaat tataagtcga caattaagtt
    24361 gataaaacag ttagactgga aacttatagt tacagtatta gctatcttta tttttgggct
    24421 tgttatactt agtagtgcaa cacatgctaa ttcaacaggt tcatataatc aattgattaa
    24481 acaaggatta gcttttgtac ttggtatagg aatgattata gtcatattat tttttgacta
    24541 taaccttcta ggcaggtatt ataaagcgct ttatataata agtcttattt tgttagccat
    24601 agttttatta cctggaattg ggacagtaaa aggtggagct aggtcgtgga ttaacttagg
    24661 accacttgac ttgcagacat ctgaaatagt caaattaaca tttgtactaa gttatgcaaa
    24721 gatacttgaa tccaaaaagg ataaattaaa tacgttaaag gaagttatgc ctgttgtagt
    24781 ttattctctt ccatttatcg gattattgat tgcacaacca gacttgggaa caggaattgt
    24841 attttgttgt atgatatttg caatgctgtt cacagcaggt cttagttcaa agttgataaa
    24901 aagaggaata atcatactat tagtgtcaat gccactgatg tatttaatga tggcagacca
    24961 tcaaaaggta agaatagaag cttttctaaa tccagaagat gtaactctaa aaggaaacta
    25021 tcaagttatg caatctctga ttgcaattgg ttctggaggc gtaactggta aaggacttta
    25081 caatggaagt caaaatcaag aagatttttt acctgtacaa gatagtgatt ttatatttgc
    25141 tgttgtaggt gaagaacttg gtgtaatagg aatggcagtg ttgataatct tatttatgat
    25201 attcctatta aggcttttag caattgcaag agatgcaaaa gatttttatg gaaccctcat
    25261 agtggttggt gttatgggaa tgtttggtta ccagataatc caaaatatag gtatgacggt
    25321 tgcgttaata ccagtaactg gagtaacatt accttttgta agttatggag gaagttcgtt
    25381 attgacatct ttagcaaact taggattagt actaaatgtg tgtatgagaa gaaagaaaat
    25441 aaatttctaa atttaaatac atctaaacat aaattgagga gattagatat atgaatatag
    25501 cattagtagc acatgaccaa atgaaaaaca ctatggtcgg attttgtata ggttatgaat
    25561 caattttaaa aaagtatgga ctatatgcaa ctggaactac aggaaaaaga ataatggatg
    25621 aaactgaatt aaatataaat agattagcat ctggtccttt aggtggagac cagcaaatag
    25681 gttctttaat agtaacacaa gaaatagatt tagttatttt tttaagagac ccattgacat
    25741 ctcaggctca tgaaacagat atacaagcgt taataagact ttgtgatgtt tatcatgttc
    25801 caatagctac aaatctagct tcagcagaaa tatttataaa agctcttgat agaggagaac
    25861 tatcatggag agaagttaga aaaagcaaaa gtcaacgtat ttaaaaaata ttaatattac
    25921 agtctaaaga aacagatata taagtgttta atgccctagt ttttctgttg gaataaatct
    25981 agtacctgta gaaatcttat gaaagattta aatgatgaag agctaaattt gagattaaat
    26041 aattaggaaa tagattatag atagagtagt agaatttaaa ctattactct attttaataa
    26101 tatgaaatag aatcaagtct attattatat atatttaaac ttataggtaa tactatttca
    26161 accaagtaaa tgttgttatg acaacattaa taaaacaaaa atttattttt aaaatttgat
    26221 aaatagctgt atattagtat ttatagatta aatattaata tacagctatt tattattttt
    26281 aagtctattg tagaatataa accttgtatt atagagtact atgtaatatt atagtaaata
    26341 taagtactat acattataga gtagtaggtg aaaaggcggt ggtacataat gcagttaaat
    26401 aaagaagtgt taaaaggtta tatcgatata ttaattgttt ctatattaga aaagaaagat
    26461 tgttatggat atgaaattgc aaagcaagta agagaaagaa gtgagtttga attaaaagaa
    26521 ggaacaatgt atcttgcact taaaagaatg gaatctaaaa atttaattaa atcttattat
    26581 agcaatgaac aaagttcagg tggaagaaga aaatattata atttaactaa tgaaggtaaa
    26641 gattttttag aaataaagaa acaagaatgg agatttatta aaaaggttat gaatcaattt
    26701 ttgggagagg tggattaatt tatgaaacta atagatgaat atttagataa actatataaa
    26761 aaatgtgaca ataaaagtac tatagaatta aaacaggaaa tgagatgcca tcttatagaa
    26821 tctgcaaatg aatttaaatt agaaggatta gatgaagaag aagcatgtaa aaaagcaatt
    26881 gaaagatttg atgatggtga tgaaatgcag tatgaattat gtaatataat aaaagaacta
    26941 tctttatcat tggataggca taaatcaatt gtaatggggt ttaaaaaagt attaggttac
    27001 ataagtataa ttgcattttt aatttctgga tttatgtggt attacaataa tagtttacaa
    27061 cataatatgt ataatttagg aaaagaactt gatggagaaa taaagcaatt ggcagaaaga
    27121 catgatatga caaagattgg tgaatataag ttagaacttg aaaaaatact tgataaagat
    27181 aagtatagta aagtcaaagc tcttagacta tatgtaatag atatgaaaga tggaaacact
    27241 aatttaagtt cttctggatt gaatgctaat atggtatatg agagggaagc agattataat
    27301 aacataagta attttataca gcatttagga tataatggaa aagatttttt agacaagaat
    27361 gggaatatag taaatccaga tattttctta gaatatttct tttattttga atcggagatg
    27421 ttaattccag tagcattcgt atttggatta ttatgtataa tagcgtattt tatattaaga
    27481 tttaaaattt ctttaataaa aaataataat taaataattc accaaataat tttaatttgt
    27541 agagtaaata agatgtttca aaataaaatt tgaatcatct tattttttat tgtttgttat
    27601 attcttttct tttatatttc tccatagtgg attgtatcta tacataatca cattaaaaac
    27661 aacacaacat atgttaactg gaacgcagca gagggagcca atactgctta tacattggtt
    27721 actaaaaaag gtgcttgttc tggaatgtgt aggagcttga aagctttatg tgatgctatg
    27781 ggaattgaat gttattatgt acatagtaca acaaatgacc atcagtggaa tcttattcgt
    27841 tttgaggatg gacgtttgta tcatgtagat attgatgcca acaaatcagc aggagcagac
    27901 atcatataca agagtcttac tcacccatta ccatttgaca agaaagcata tccagctgtt
    27961 ggcgaagtag taaagacaca gaatccaacg ccaactgtta agaaacctac gccagctaag
    28021 ccttctgcgc cagttggacc tgggaatcac actatgatag cagatcaaca aggggcaata
    28081 cttgatgaag tttatggaaa taatctacgt ccaaaaattg aaatgtatga gggagttccg
    28141 tgtagtttaa tcaactcatc aataaataca attaagttta caagcaaaaa ttcaaattta
    28201 gacccatcaa agcatacagt tactgtaata gggtttatga atacaccata tggtatggtt
    28261 gcattagcac cagagagcaa acctgttagt tatccttttg agtatgataa ggcattttct
    28321 ttagatttta ctggtaagaa cctttctgca gcagaattat caaaaaagtg taaaaaaata
    28381 atataattat taaagtagaa attggtgaaa caacatctaa ttctggaata gggctagtag
    28441 catatactag atacgtgaat taatacaagt atactagtat acaaatagaa cagactataa
    28501 ataatgcacc ctgtgcgaaa gggtacattt attattatat tagtcatttt ggaaaataaa
    28561 aaaggagttg aacttaaaat tagattagaa aaattaattt tgagacaact ccattttaat
    28621 ttatttttaa atataaaatc atttatttta aataatatta tgtttgatta atggctattt
    28681 aactaatttt gcaaattctg caccaaatgc catacactct tttaatgtct cttcactagg
    28741 gaagaatttt ttcttaagag attctacagg cattttgaat gacatagatt taagtaaagt
    28801 ctcagccata gttatacctt caccactcca accaaatgaa ccaaatactc ctgctatctt
    28861 gccttggtta gccattgggt ctataacaga gaataaatcc cacataggtt taaccatagt
    28921 tttgtttata gttggagagc ctagtaatat tacttttgac actactaatg tatcgtgcat
    28981 ttctgttaat gtcatatctt ctaaatcata taattcagcc tttgcacctt ctttatcaag
    29041 tccttctttt atctttttag ccatctctaa agtattacta taagctgata agtagaatat
    29101 agaaacttga ttttgattag ttgtattaac agcttctgta gaccactcaa catatctttt
    29161 aacagcagcc attgggtctt tagtaagcat tggaccatga gaagtcaata tagtatcaaa
    29221 ttctatgttt agtcctacca ccttatctac agcacttaaa acatgtttag caaatggctt
    29281 aactatacaa tcatagtagt gtttagctga ttttagataa tcttcactat tcactacctc
    29341 tgcatcaaca cttgcaaagt gacaaccaaa tgcatcacaa gttaaaagag tcttgtcctc
    29401 ctcaatgtaa gtaaacattg tatcaaccca atgtaagaat ggagcagtta taaatctaag
    29461 atttctttta ccgatattta aaatttcacc atcttttatt acatgacagt taaaaggtct
    29521 atttatttga ccatctaagt atagtttagc agctttagta caataaactt ctatattagg
    29581 atttatatct aataagtatt ttaaactacc tgcatggtct ggttctgtgt gatgaattac
    29641 aacatagtca attttagcta tatcagtaac ctctgataaa ttacttaaaa actcatcttt
    29701 aaaatttgcc tttacagtgt caaataaaac cgttttttcg tccttgatta aataagagtt
    29761 gtaagttgtc ccaaactcag tttccattat tatgtcgaat acttttaaac cttcatctac
    29821 tactccagta aagtagatat ctttttttac ttcaaaaact ttactcattt ttaaccctcc
    29881 taaaatttat cattatataa gtaacatacc ccattttgag gtgaatgaaa caaaagatgt
    29941 catgataagt catgtaaaac aatagtttta tgtttataat atatcatact ttgtaaaaaa
    30001 attgtatttc aataaaaatt taatagaaaa tattcttaaa tatttttagc tattctattt
    30061 tgtgaataat atgtatttta aattcaatat tttagttata tttttgatag aaaatatttt
    30121 atcgtgtata atattactta taaataagaa taaaaataaa gagcttgttt ggaggaattt
    30181 taataaatga ataaagtaga cttgaaaaaa atcttaaaaa aagtagaaaa gcctgctaga
    30241 tatcttggaa atgaaataaa ttcaattcat aaagatacta gtaatagtga attgataaga
    30301 tatgcacatt gctttccaga tttatatgaa gttggtatga gtcacttagg gagtcatata
    30361 ttatatgatg taataaataa ggatgaagat gtattttgtg agagagtgta ttctcctgca
    30421 gttgatatgg aaaatataat gagagaaaaa agtataccat tatttgcatt agagtcaaga
    30481 gagcctataa caaactttga ttttgtcact tttacacttc aatatgaact ttcatacact
    30541 aatatcttaa atatattaga tttagccaat ataccaatat taaaagaaga aagaactttg
    30601 gaagacccat ttataatggt tggtggtcct tgtgcttata attccgaacc attagcagat
    30661 tttgtagatg tagtaatatt aggtgaaggt gaagaggtta atttagaagt agtaaatgag
    30721 tataaagaat ggaagaaaaa taaaacgact agagaagatt ttttatataa aatatctagc
    30781 atagaaggag tatacatacc aagtttttat gatgtcaagt acaatgaaga tggtacagtt
    30841 caaagtgtaa ctcctaatag agaagggatt actaaaaatc ctactaaaag gataataaaa
    30901 gatgttgaga ctgtagatta tccagaaaaa ctaatagtac cttatataga tactgttcat
    30961 gatagaatag tactagaatt atttagagga tgtactagag gatgtagatt ctgtcaagca
    31021 ggtatgatat acaggccaat aagagaaaag agtgttaaaa gacttaaaga aatattagat
    31081 aaattagtta agaatactgg atatgatgaa atatcattat catcacttag tacgagtgat
    31141 tatagtaagt tatcagagtt gactgattat ttggtagatg aatatgcatc taataatatt
    31201 gggatttcgc ttccatccct tagattagat aatttttcaa tggaaattgc tgataaaata
    31261 caacaagtaa gaaaatctgg acttactttt gctccagaag caggaactca aagacttaga
    31321 gatgttataa ataaaggtgt tacagaagaa gatttggaaa atgcaacaga aagagctttt
    31381 gagatggggt ggaatagtgt taagttgtat tttatgattg gtttaccaac tgagacttat
    31441 gaagatttag atggtatagc taaattggct tataaagtta ttgatattta tagaaaagta
    31501 aatggaggaa agctaaagag aagttttagt gttacagtga gtacttctac atttgtacca
    31561 aagccattta cacctttcca atggcatggt caagatacta cagaagaagt tattaataag
    31621 cagagacatt tagtaaataa attaagaaat aacaatataa aatacaatta tcatgattca
    31681 aagactagtt tgatggaagc agtagtagct agaggcgata gaagaatagg aaaagttata
    31741 tatgatgcat ttagactagg tgctaaattt gatggatggg cagaacattt taatttagat
    31801 atatggaaag aagctatgga aaagaataat ctttctatag atttttatgc acatagaaat
    31861 agaaactatg aagaggtatt tccttgggac catatagatg taggtattag taaaaaattc
    31921 ttaattagag aagacgaaaa tgctaaaaaa gaaaaaatca cttctgattg tagacataac
    31981 tgcaatggtt gtggaataaa tatacatgat ataggaaggg ggctttgcta atatgagtaa
    32041 gataataaga gtaaaattta aaaaggaagg cgacatgata tatatatcac atcttgacct
    32101 tcagagattg ttacaaagag cgtttagaag agctgaaata aacttatcac attctcaagg
    32161 atttaatcct caccctaaaa tgagttatgg caatgctttg gctttagggg cagaaagtca
    32221 aggtgagtat gtagatattg aaatagaaga agatgattta tcagtagagg aatttttaaa
    32281 taaagtatca attcaacttc cagatgggat agattttatc aaagcaaaag aaattgatag
    32341 acaaacacct tctctttctt ctgtgataga ctatggagaa tatttgttta atatagattt
    32401 gaaaagacct atgacgaaag agtttgtaaa aagaaaagta attgacttta tgaataataa
    32461 agaaataatc ataacaaaaa agaataaaaa aggtaagatg gttgaagtag atataagacc
    32521 tatgataaga acctttgatg ttttaaattt agaagatgaa catattactc ttactgctac
    32581 tatagctaca ggctcaaaaa ctaatttaaa tactaatata cttataccaa agatattaga
    32641 gatgtttgaa cttgatattg acccattaga tgttgatata ttgagaagag atttatatgt
    32701 attagaagat ggggaattag tgactcctat gtaagctcga gaggtgaagt tgtgaaaaag
    32761 atagttattg agtccctaat agggtcccag aaaacagcag tacttgaaga tgaaagatta
    32821 acagaacttt ttgttgaaga caatttaaat aagaagactg tatccaatat atatcgtgga
    32881 atcgtaaaaa aagttatacc aggaatagaa gcttgttttg tagatattgg atttaagaaa
    32941 cttgcttatt tacaattaaa aaagggaagt actataaaat ctggtcagga tattttggtt
    33001 cagataaata aagaagaaat aggaactaaa ggcgcaaaat taaacactga gattagtatt
    33061 tctggaaggt atattgtcta tataccttcc aatgatagaa ctacaatatc aaataaaatt
    33121 actgatgaaa aagaacgatt tagattaaaa aaaattacta aggcagtaaa taaggaaaat
    33181 ctaggattaa taataaggac tgaggctcaa ggttgcactc atgatgaaat aaagaaagat
    33241 atagaagagt tgaaattaaa gtatgaaaac attttaaaag aatataaact tggtataggt
    33301 ccgaaattat tatataagtc tttagatttt gcaactaaat atgtaaaaga taatgtgaat
    33361 gatgatattg aatctataat taccaatagt tatgataagt actctgaact taagtctatt
    33421 ttaagaggta tagataagac ttatgttgat aaactgtgtc ttgaggaaaa tagagatgta
    33481 tttgatttat acagaataga atctaaaata gaaaaattat taaataaaaa agtatggtta
    33541 aagagtggag gttatttaat aattgaaaaa acagaagctc ttacggttat agatgtaaat
    33601 acgggtaagt ttattggaac tggtaaactt gatgaaaccg tttataaaac taatcttgaa
    33661 gctgcaaaag aaattgtaag acagcttaga attagagaca tagctgggat aataataatc
    33721 gattttattg atatgcataa aaagaagcat caaaaggaga ttttacacat cctagaagaa
    33781 gaatttaata aagataaaag aaaagctgaa gtattaggta tgacaaaact tggtttagta
    33841 gaagttgcta ggcgtagaga aaaagaatcc attgataagt attatttaat gtcttgtcca
    33901 tgttgtgatg gtgaacaaac aataaaatct gtacattata tactagatag tattgaaaaa
    33961 gaaattatga gaataagtga acatactgtt tacaaaaaca taatggtaga atttaatgat
    34021 tttatttttg aacaaataaa agaatattat atggatataa ttgataaaat tggtgaaaaa
    34081 tataatataa agatttcttt aaacgcaaat agtacattaa agcacaataa aaccaatgtt
    34141 attttcgata aaatagttga caataaaatg taaatatttt ataattaata attgtaatgc
    34201 cgcacagtaa aggtttaaat cttcaaatga agtaccttgt actggcgagt ttgaggccga
    34261 ggaggtgtat ttaatgtacg ctatagtaaa gactggtgga aagcaatata aagtttctga
    34321 aggtgatgta ttattcgttg aaaaattaga agctaacgca ggtgatgttg ttactttaaa
    34381 cgaagtatta gcttgctcta aagatggaga attaaaatta ggttctccag tagtagaagg
    34441 agcatctgtt caagctaaag ttgttgaaca aggtaaagct aagaaagtta tagttttcaa
    34501 gtataaagca aaaaaagatt acagaagaaa acaaggacat cgtcaatcat acactaagat
    34561 agttgtagaa aaaataaacg cttagttatt ggttaagtta tgataaaagt taaatattat
    34621 tataatgatg atttcttgtt gcaaggtttt tgtttaaagg gacatgctga ttttgctgaa
    34681 ataggctatg atatagtatg tgctgcagtt acatctaatg ctattgcagt tataaattct
    34741 ttagacaaat tacagaagat tgaatttgaa aaagtagtag gagaagaagg tcatatagag
    34801 tgtatagtca aagatacaca tgtaaaagac gcacaattat tactaaatca ctttcaatta
    34861 gcggttaaag aaattaaacg ggaataccca aaaaatataa aaattttaaa aaagtagggg
    34921 gtgactccat atgttaaaca tgaatttaca attattagca tctaaaaaag gggtaggttc
    34981 ttctaaaaat ggtagagatt ctatatctaa aagattaggt gttaagagat tcgatggtca
    35041 attagtaact gctggaagta taatagttag acaaagagga actaaaatac atccaggaac
    35101 taatgttgga aaaggtagtg atgatacttt atttgcatta gttgatggta ctgttaaatt
    35161 cgaaagaaaa gacaagaaga gaaaaaaagt tagtatatat ccagtagcaa tagctgagta
    35221 atatacataa aaggagtgtt gaggcactcc tttttcttat aatactgatg aatttataat
    35281 ggtatttttg gtaatgctaa ttaaaagttt ttaattaagt aggtgaagaa tttgtttata
    35341 gataaggcaa gaatatttgt taaggctgga aatggtggaa atggttcggt agcatttaga
    35401 agagaaaaat acgttccagc tggtggtcca gacggtggag atggtggtcg tggagcaagc
    35461 atcatatttg aagttgattt ggggcttaga actctaatgg actttaagta ccagaaaaaa
    35521 tatcaagctc aaaatggtgg cgacggaagc aaaggaaaaa gagctggtaa aaatggagaa
    35581 aatttagtat taaaagtacc agcaggtaca gtaataagag atgaggctac aggtcttgta
    35641 ttagcagatt taaaaaaaga aggagataca gctatagttg caaaaggtgg tataggtggt
    35701 aaaggtaacc aacactttgc aaatgcagta agacaagctc cagcttttgc aaaatctggt
    35761 acagatggag aagaaagatg gataactctt gaacttaaaa tgatagcaga tgttggtctt
    35821 ttaggatttc caaatgtagg taaatcaaca tttctatcag ttgtaactaa agctaaaccc
    35881 aaaattgcaa actatcattt tacaacactt acaccaaatc taggtgttgt tcaaactaag
    35941 tttggagata gttttgtact tgcagatata cctggaatta tagagggtgc ttctgaaggc
    36001 ataggattag gtcatgagtt cttaagacat gttgagagaa caaaggtatt gatacatatt
    36061 gtagatatat ctggattaga gggaagagac cctattgaag attttgacaa gataaatgat
    36121 gagcttaaat tgtacaatga aaagctttca aagagaccac aagttgtagt tgcaaataaa
    36181 tttgatatat tagaagatga gagtaagttt gagaagttta agagtgaatt agaaggcaga
    36241 ggatatacag tatttaaaat gtcagcagca actcgtcaag gcatagatga agtaatagcc
    36301 tatgtttcta aaatgctaaa agaagtagaa gatgtggagc tagtttcaga agaagaaatg
    36361 tatagaccag aacttgatat tggtactgaa gaagagttaa gtatagatat tgaagatggt
    36421 gtgtatgtag ttacaggtaa agcacttaga agaataatgt attctgtaaa ttttgatgat
    36481 atggaatccc ttcaatattt ccaaaaagct atggagagtc aaggtgtttt tgataggctt
    36541 agagaaatgg gaatagaaga tggagatgtg gttaaaattt atgaattaga atttgagttt
    36601 tacaattaat ttaaatatag attatagata atttaagtat agaattatag aggtgagaat
    36661 atgttaaaag gaaaacaaag ggcgtattta agatctttgg caaatacttt aaagcctact
    36721 acacaaattg gtaaagaagg tgttacagag agtttcttag agcaattaga tggtatgtta
    36781 agaactagag aattagtaaa agttactata ttagaaaatg caggtcttga tactaaagaa
    36841 actgctaatg cagtatgtga agctttaaga gctgaatttg tgcaggctat tggttttaaa
    36901 tttactattt acaagaggaa tattgaagag cctaaaatac tttttccagg acatgaacag
    36961 gcgaaagcaa agactaaaaa taataatgtt acaaaaaagg gtaaaccaac taaaagagct
    37021 gttagataat agtttatatc cttttaggga aataagttaa aaaaatagtg acaattggaa
    37081 gaaattgtat tatactatta aacataaatt taaattagca atcttatcaa gagtggcaga
    37141 gggactggcc ctgtgaagcc cggcaaccag tatatttaat tatacattgg tgctaaatcc
    37201 tgcagcattt atgctgaaag atgagtataa acccgtcata tggcttgagt ttatactcaa
    37261 gcttttttta ttggtatatt tatatatata aataatttaa tataatcaaa atgatggggg
    37321 tatgttacag tgaagatagc aatattaggg tatggaacag taggaagtgg attaattgat
    37381 ttaatagaaa ataatacttc aaaaagagat atagaagttg ttggaatttt agttaaaaat
    37441 aaagaaaaac acaagtataa gaagtatttt gataagataa ctactgatat agaagatata
    37501 tttgacaagg atattgatat actagttgaa gtaattggag gattaaatcc agcttttgat
    37561 tatgtaacaa gagcgttaaa taaaaaaata catgtagtga cagctaataa agatttactg
    37621 gctgaaaaag gtagtgacct tatagaatta gctaacttaa ataatgttag catcaagttt
    37681 gaagcatctg tggcaggtgg tattccagtt ttaaaaccat tgatagaatc actggaaggc
    37741 aacaatataa agagcataaa cgctatttta aatgggactt gtaattttat attatcaaag
    37801 atgtatgatg aaaatttacc gtatgatgtc gctttaaaac aggcacaaga gttgggattt
    37861 gcagaagtta atccagatgc agatgtttta ggatatgata gtgctagaaa gttatctata
    37921 ttgtctactt tatcttatgg aaaaatagta tattggaaag atttattatt agaaggaata
    37981 acttcaatag atgaaaaaga tatagaatat gcaaaaaaat taaattgtaa gataaaattg
    38041 gttgctagaa gtaagtatga atcaggagaa gtaagtggat ttgttagacc agcattagtg
    38101 gacaataata atatgctttc aaagatagat aatgagttca acgtagttat tttagtaggt
    38161 gattcagtag gtgaactttc ttttgtaggt aagggagctg gaagaggtgc aacaggaagt
    38221 gctgtttatg cagatattat agatataata gataatagaa gttcaaatat aaagtctttt
    38281 tcaaaaggga aattggattt aagtggactt atagaagatg agtgtagtgc agtagttaga
    38341 tttggtgggt ataataataa agatggaatt ttagaatgtt tagataaata tgtagatagt
    38401 tatgatatta tagatgatga agaattggcg atttttgtaa gtgcaaagtc agaacatgag
    38461 attgatagag ttttggaaat tgtaaaaaga aataattaca gtgaatcagc taaaaaatta
    38521 ttgaagatag attaatatgt ttatgcaaga attatgaaaa tttaataggg tgtctcaaaa
    38581 tagagagata agcctttttt ccagtgttga aataataaaa atatatttga ttttataaaa
    38641 aaagagtgtc tcatgaacta aaaagttcat tttgagacac tcttttttaa tatctggtga
    38701 attaataaga taatagaaaa atgtatacgt tataattaac caataaagaa attttcacta
    38761 gcttcattaa tagcttgctg agttaccctt atatatcgct gtgtagtggc tacagagctg
    38821 tgatttaaaa gttctttaac caactctaaa ttattgttac tcttttcaaa ttgagtagta
    38881 gcatagagct ttctaaagct atgtgtactt atattataca gatttaagta tttacaaatt
    38941 atcctcaact gtttttgaat agcctttgtc tttatattta ctaagtagtc atttgattta
    39001 agattatact ttcttgcatg ttcatttatg gactcaatta tatttttatt aactggtcta
    39061 tactgtaatt tgcccgtctt tttttcattt atttccaaca tattaccttt aaaattacct
    39121 atctttaatc ttaaaatatc tgatattctt agacctaaat tagcttctaa cattagtgcc
    39181 aatgcaactt taggattttt tctaaatctt ttttcaacgc cattttcact ataagtaaag
    39241 ccagtatgta atagttccat tatcttttta tattcgtcta tttctatagg tcttgttgcc
    39301 attttcaacc actcctctta gtttgatttg gtaagtttag gtcggtattt attatataat
    39361 ataaatgaaa tattata
//
LOCUS       DAYATN010000021        36346 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.021, whole genome shotgun sequence.
ACCESSION   DAYATN010000021 DAYATN010000000
VERSION     DAYATN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.021"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(207..1316)
                     /locus_tag="ACGI8A_001089"
     CDS             complement(207..1316)
                     /locus_tag="ACGI8A_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179095.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRNLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            1574..2311
                     /gene="cas6"
                     /locus_tag="ACGI8A_001090"
     CDS             1574..2311
                     /gene="cas6"
                     /locus_tag="ACGI8A_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HFH0179096.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPINFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            2316..3791
                     /locus_tag="ACGI8A_001091"
     CDS             2316..3791
                     /locus_tag="ACGI8A_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179097.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENRLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYSFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKRMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            3811..4824
                     /locus_tag="ACGI8A_001092"
     CDS             3811..4824
                     /locus_tag="ACGI8A_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HFH0179098.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            4840..5637
                     /gene="cas5"
                     /locus_tag="ACGI8A_001093"
     CDS             4840..5637
                     /gene="cas5"
                     /locus_tag="ACGI8A_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HFH0179099.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYIKMGKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVKLKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            5664..8072
                     /locus_tag="ACGI8A_001094"
     CDS             5664..8072
                     /locus_tag="ACGI8A_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFH0179100.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            8513..9460
                     /gene="prmA"
                     /locus_tag="ACGI8A_001095"
     CDS             8513..9460
                     /gene="prmA"
                     /locus_tag="ACGI8A_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFH0179101.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            9479..10237
                     /locus_tag="ACGI8A_001096"
     CDS             9479..10237
                     /locus_tag="ACGI8A_001096"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFH0179102.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEIILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNSVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            10239..11537
                     /gene="mtaB"
                     /locus_tag="ACGI8A_001097"
     CDS             10239..11537
                     /gene="mtaB"
                     /locus_tag="ACGI8A_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061712 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HFH0179103.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            11580..11930
                     /locus_tag="ACGI8A_001098"
     CDS             11580..11930
                     /locus_tag="ACGI8A_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFH0179104.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            12093..12272
                     /gene="rpsU"
                     /locus_tag="ACGI8A_001099"
     CDS             12093..12272
                     /gene="rpsU"
                     /locus_tag="ACGI8A_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFH0179105.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            12302..12745
                     /locus_tag="ACGI8A_001100"
     CDS             12302..12745
                     /locus_tag="ACGI8A_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFH0179106.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            12856..13353
                     /locus_tag="ACGI8A_001101"
     CDS             12856..13353
                     /locus_tag="ACGI8A_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFH0179107.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            13546..14109
                     /gene="raiA"
                     /locus_tag="ACGI8A_001102"
     CDS             13546..14109
                     /gene="raiA"
                     /locus_tag="ACGI8A_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFH0179108.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            14322..14558
                     /locus_tag="ACGI8A_001103"
     CDS             14322..14558
                     /locus_tag="ACGI8A_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HFH0179109.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            14571..15914
                     /gene="yqfD"
                     /locus_tag="ACGI8A_001104"
     CDS             14571..15914
                     /gene="yqfD"
                     /locus_tag="ACGI8A_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HFH0179110.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            15853..16968
                     /locus_tag="ACGI8A_001105"
     CDS             15853..16968
                     /locus_tag="ACGI8A_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFH0179111.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            17025..17486
                     /gene="ybeY"
                     /locus_tag="ACGI8A_001106"
     CDS             17025..17486
                     /gene="ybeY"
                     /locus_tag="ACGI8A_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFH0179112.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            17492..18211
                     /locus_tag="ACGI8A_001107"
     CDS             17492..18211
                     /locus_tag="ACGI8A_001107"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFH0179113.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            18330..18731
                     /locus_tag="ACGI8A_001108"
     CDS             18330..18731
                     /locus_tag="ACGI8A_001108"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFH0179114.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            18795..19688
                     /gene="era"
                     /locus_tag="ACGI8A_001109"
     CDS             18795..19688
                     /gene="era"
                     /locus_tag="ACGI8A_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFH0179115.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            19700..21079
                     /gene="mgtE"
                     /locus_tag="ACGI8A_001110"
     CDS             19700..21079
                     /gene="mgtE"
                     /locus_tag="ACGI8A_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFH0179116.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            21152..21307
                     /locus_tag="ACGI8A_001111"
     CDS             21152..21307
                     /locus_tag="ACGI8A_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HFH0179117.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            21273..22046
                     /gene="recO"
                     /locus_tag="ACGI8A_001112"
     CDS             21273..22046
                     /gene="recO"
                     /locus_tag="ACGI8A_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFH0179118.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            22039..22638
                     /locus_tag="ACGI8A_001113"
     CDS             22039..22638
                     /locus_tag="ACGI8A_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HFH0179119.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            22798..23676
                     /gene="glyQ"
                     /locus_tag="ACGI8A_001114"
     CDS             22798..23676
                     /gene="glyQ"
                     /locus_tag="ACGI8A_001114"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFH0179120.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            23676..25742
                     /gene="glyS"
                     /locus_tag="ACGI8A_001115"
     CDS             23676..25742
                     /gene="glyS"
                     /locus_tag="ACGI8A_001115"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFH0179121.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(25908..27455)
                     /locus_tag="ACGI8A_001116"
     CDS             complement(25908..27455)
                     /locus_tag="ACGI8A_001116"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HFH0179122.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEKLFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNKEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            27735..28502
                     /locus_tag="ACGI8A_001117"
     CDS             27735..28502
                     /locus_tag="ACGI8A_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFH0179123.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            28785..28982
                     /locus_tag="ACGI8A_001118"
     CDS             28785..28982
                     /locus_tag="ACGI8A_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0179124.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            29011..30087
                     /gene="vorB"
                     /locus_tag="ACGI8A_001119"
     CDS             29011..30087
                     /gene="vorB"
                     /locus_tag="ACGI8A_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFH0179125.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            30090..30809
                     /locus_tag="ACGI8A_001120"
     CDS             30090..30809
                     /locus_tag="ACGI8A_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFH0179126.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            30822..31361
                     /locus_tag="ACGI8A_001121"
     CDS             30822..31361
                     /locus_tag="ACGI8A_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFH0179127.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            31518..32576
                     /gene="buk"
                     /locus_tag="ACGI8A_001122"
     CDS             31518..32576
                     /gene="buk"
                     /locus_tag="ACGI8A_001122"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFH0179128.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            32594..33487
                     /locus_tag="ACGI8A_001123"
     CDS             32594..33487
                     /locus_tag="ACGI8A_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HFH0179129.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            33517..34725
                     /locus_tag="ACGI8A_001124"
     CDS             33517..34725
                     /locus_tag="ACGI8A_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFH0179130.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            34749..36008
                     /locus_tag="ACGI8A_001125"
     CDS             34749..36008
                     /locus_tag="ACGI8A_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0179131.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        14550 a         3759 c         6627 g        11410 t
ORIGIN      
        1 taaataaaag ttgcctttta aattatcgag attattttta tctagaaaat caataacata
       61 aatagcatta ttgaaagtaa ctaaatggct actagcatct aacttttggt tttcatatgg
      121 atagatattt atttttttat taagctgtct ttctaaaaaa tcttttaact cttccataaa
      181 gtctccttaa aaataagatt atattcttat cttatagcta aagtagtttc tttgtcaaat
      241 atgtgtactc tactattgtc aactgaaagt gttaagtcat caccaacatt aattctgttt
      301 ttactgtcaa atctaattgt tatcgtattt tcgcctagtt ttaagtgagc ataagtttct
      361 gctcccataa gttcttttat ttcaattctt gatttaaaac aagtatctgg attagattta
      421 ataaaagtat cttctacatg aatatcttct ggtctgatac ctataacaac ttcattatta
      481 atataattgt cttttattaa atcatagtgt ttttctttat tcaatttaag cttaatatgt
      541 tcattttgtg cataaatttc atcattttct tctattaata ttacatcaat aaaattcatc
      601 tgtggtgctc caataaatcc tgcaacaaat atattagctg gataatcgta tacatcttgt
      661 ggagttgcaa tctgctgaac tactccatcc ttcattacta cgattctatc agccatagtc
      721 atagcttcaa cttggtcgtg tgttacatag ataaatgtag ttccaagctg ttggtgtagc
      781 ttagttatct ctgttctcat agcagttcta agttttgcat ctaagttgga tagaggttca
      841 tccattaaga ataccttagg atttctaact attgcacgtc ctaaggcaac cctttgtctt
      901 tgtcctccag ataatgtttt aggtttttta tttaaaagag gtagtaagtc taatatttta
      961 gcagcttctt taactttttt gtctatttca tctttaggca aatttctaag tttaagtgca
     1021 aatgccatgt tttcgtaaac agataagtgg ggatataaag catagttttg aaaaaccata
     1081 gctatatctc tatcttttgg ttctatatta ttgacaagtt tatctccaat gtatagttct
     1141 ccttcagaga tttcttcaag accagcaatc attctaagtg tagtcgattt accacaacca
     1201 gatggtccta ctaaaacgat aaattcttta tctttaatgt ctatatttat attttttact
     1261 gcgttaaatc catttgagta aagttttgat atatttttta aaattacttc tgacataata
     1321 tcctccttga tatttaaatt ttaattatat agtatcaaat ttttttatta tattaaatgt
     1381 gtaaaatgac taaatgtatt tgtttaatat gtatatttag tttttagcaa agaataaaac
     1441 taacagtaga atatatttaa aaaattaaaa aacaaaatta aatttattat caaaaaatca
     1501 ttttatgata tgttttattt ttatattaca aatagagaat aatgaattat actagaaagt
     1561 aggaggtgaa aatatgagca gaatttcaat ttttatggaa tcatgtaatg gcaaatttcc
     1621 aaatgaaaat tcaatgttgt cagtttcctt tataaaaaat attttaaatg cagaaaacaa
     1681 agattttgct aaaaacatat ttaactatgg ggaagaaaga aaaaacaata agcaaataaa
     1741 ggatataaat acagccattt acataccaaa tcttataaga agcaaaaatg agttacttgt
     1801 tgatggagat ataatattca atataagttt ttataattat tctatgttca gtaaattata
     1861 caatggaatt ttaaaagtaa aggagctaga atataaaggt tatagattta aaataaaaaa
     1921 tataaaaata cataaagaac acgagataaa agaaaatggt gttatattta aaactatgtc
     1981 tcctataata gtaaagaata aagaaggtaa atatttagat gtagaagatt caaattatat
     2041 agaatgttta aactatatag ctaatctaac tctggagagt ataagaggta gtgggcttag
     2101 aaagccattg gaatttatac ctattaactt caaaaaaaga gtgttaaaag aaaagattcg
     2161 tggatttaaa gtgagagaat attactatat aaatgcttat gcaggaacat tttttttaaa
     2221 aggagatatg gaagatttaa atgcactgta taaaatggga attggatata gaaggacaga
     2281 aaatgcagga atggttgata tattaaagta gcgaggtgag agaaataaaa gctaaatttt
     2341 atagatcagg tgatattgta agagatattg gcattgttta cttttatgaa ttactaatgg
     2401 atttaaaaaa cgaactagaa aaaaatgatt ataagctaaa atttacttgt gagttaaata
     2461 gaaattattt atctttagaa tctgcagaat atatagaccc taagtatata tctgattata
     2521 tattagaaaa tcgacttttt aaggtattta aaaatgggaa aaaggaaacc ttagaaaaaa
     2581 tatttagtaa tatagataat ttaacgtatg taaattttat tgaagaatta gataaatctt
     2641 ctgcaactgc taaacaaaag gaagatatta aaaaagattt taaaaacagg aggtttccat
     2701 atgttagaaa tagtggtaag tatggattaa acacgagttt agaaaatatg gaaacaaatg
     2761 taaaaagaat agtagatact gtaattaaat caaatataga gatagatgaa atagataaac
     2821 ttgatttgac acaatatgaa gaaaaagata gtgtatgtaa tgtttgcaat ataaataaaa
     2881 caacaaaact agatattgat ttaagagaac gaaaggactc taagtataac tttttattta
     2941 gaggtgcaga aaaatcaggc tttaaaagaa gtggacaagt tgaaagtaat atttgttttg
     3001 agtgtgaatt tttcaactta atgtgtcttt tatatataaa tttaaaaaga ccaatggttt
     3061 ttgcatatac gaatgattta agagaattag catttttaaa tcataagata atgcttaaaa
     3121 gaaaaatgta ttcagataaa tcattttata aaaaactttt gcatgaaaaa ataagttcaa
     3181 ttagactata tagctttgat attgatacga ataaaggtat aattctaaag tttgattcta
     3241 ttatagagta taaagaatta ttaaaaaaaa ttgaacttat agatattata gataattata
     3301 atttttcaag agagccaggt aatactagaa atttgggtaa aagaatgatt gataatggga
     3361 atttatcaaa tttaaaagaa ttattacttg ataatataag tgttttaaag caatcaactg
     3421 gaacttctcg agaaatggat tttgtatcta gctcatataa tatcggttta tatataaaat
     3481 tgtgttggat tgtagatgga ggggaggagt ataaaatgaa aaactatatg gaaagtaatt
     3541 taatatattc aagagtaggt aaagacttat ttaataaaat gacagatgaa tccagaagaa
     3601 aaaatttttc tatgaaagtg attcaactac ttaagtctaa tgatagaact cagctatttc
     3661 aaactataat gcatgtgtta gtttctaatg gaatcttaat aggagaaggg tttgtagaag
     3721 gtctaatgca gagttctgaa ttagaattaa atacgaatgt aggtctattt attcaagaaa
     3781 tcatgaaata aaaaaggggg aaaatttaaa atgaatgaaa aaagagtaaa aggagtaaca
     3841 ttaactatac tgacagaaag cccagttcca ctttcatatg accaagggta tggaaattat
     3901 acaccaataa aaaaagaaca atatagagaa aaaatacatg caaaaactag tatagccaca
     3961 ataacatatg atttaagaag aatgctccac caagaatatg gttggaattt aagtaatatt
     4021 gtttttggaa aaaaaggaaa tatatatcca tctattaaga aaaatgtagg atgtgtagat
     4081 gaaaatggat tagagactga tgtatttggt tatttaatac cacttaaaga taaaggttct
     4141 ataagtaaag cttctccact tagaataata ccttttaggt cattaaatcc atataaaggt
     4201 tcaacacagc taataacaaa tagaggcttt ttaagtagtg aatttggtag aaaatattat
     4261 gatgagaagg aagaaaatga agtacctaga gatgaaaact ttccaacatc acaagcatta
     4321 gcaatagaag agactttatc agattactac gtttatacaa taacattgga gcttgataga
     4381 ataggagtag tagaagtaga agatggaaag ttacttttac cagaagaaag aaaatttatg
     4441 agtaaggagt taagagaaaa agcagtaaaa gatatattag atgcaattac tgtgtttaca
     4501 agaaacataa aacattcatc agtactatta aaaccacttg cagtaatagg tggagctttt
     4561 gataaagttg taccattttt ttgggatgat gtagattata atgctgatag tggtgagata
     4621 aatttagagg gagtcataga aactatagaa agttatagcc ttaaagaaag taacacaata
     4681 cttgctatca atgatagatt aaaaatttct aataaaaagg aattaaataa gtttaattta
     4741 agtaaatacc ctgtaaaaga gattaaaaat ctagcagata gattggaaat tggagaagat
     4801 aatatgtggt atttaaaaga gtaggaggtt ttttgaatta tgaaagtatt acaatgtaaa
     4861 cttaagcaac caactgctca ctatagagac ccaaaagtat ttcaaaatga atatataagt
     4921 actttaaatt taccatctaa gaccacaata atgggaatga taacgtattt atgtgataga
     4981 agattaaact ctgatataga tataggaata attggaacac atcatcatag agaactagaa
     5041 ttttcaagag gagaaaacat tgatttttgg aatgaatata taaaaatggg aaaaggtaaa
     5101 gataaagaaa agtttttgct taaaggaaac tactatgatt actataaaga gcataaagct
     5161 caaaatagca tattaaacta tgaagttctt aaagaagtag aactgactat atttatttct
     5221 tgcaaggatg atgaagaact tgaatttatt agaaaaaagc ttgaaagtcc atgtaaatat
     5281 gcaaatttag gaagaaaaga agattttgta ataccaagtg aaaaagggtg ttttgtgaaa
     5341 gaagttaagt taaaagaggt tatacctatg aatactcgtg atgctataaa agaaaatata
     5401 aaattaaaga atacatatgt aagagttgac ttaagagata aagagaatgt cgagactata
     5461 ataaatcaag gtgtattgat tgcattgcct cataaatata aagatttaga agcaaataga
     5521 gatgatagag tttatgagtt ttgccattat atatatgttg ataatgatgg tatatatcct
     5581 aaaaatatta aggtaaatgt atgtattgat acaaaagaag tttttacatg gctttaaatt
     5641 attttgaaag ataggtgttt atcatgaaaa atatcatgca aaaagaaata ttagctaagt
     5701 catacaattt aagaactaat caacaagaac aaacattagt tgaacatatt aaggatttat
     5761 ttgaagtatt agaatctata ttagaattaa atctatattc agataaagat gtagagatac
     5821 taaaaatatg ttgtgctttg cacgatttag gaaaaattaa ttcaattatg caacaaaaaa
     5881 tggagattaa taataaaata tcgtatagtt gctcagaaga agagaggaaa aagttagagt
     5941 ctgacaaaaa atctttaaaa aagatagcaa gacataatat attttctgga gcatttttaa
     6001 aagatatatt agaaaaaatg aacttgtcag aagaagataa gttatatata tataaaagta
     6061 taatgttaca tcatggaaac tatgaagatt atatgaggtt aagtattagt aaagttcaag
     6121 aagaaattta tgagtatatt gaaaaaggca ttttagaaaa agaagagttt aatctgaaag
     6181 atatagaaag ttatataaat agcattttaa atgtagactt taaatttggt gaagatgtat
     6241 tggattatga ttacatagat aaattaagtg aaagtatgtt tatagacagt gattataaca
     6301 atggtcaaat agatgattca gttttaaatt atagaaagtt taagtatatt ttatataaag
     6361 ggatgctgaa cttaatagac catagtgcat caagtagaca gaaaggtata aaattttata
     6421 acgattttac tgatgaagaa attgataata tgattttaaa tgagatatat aagagtcagg
     6481 gtaatttgga aaaaaataat atagagttca atactataca aaaaaggttg agagaacttt
     6541 ctggaagaaa tgttctgact gttgcattta caggttcagg aaaaacagct gcagattata
     6601 gaaagacttt taaaagaaaa ttctttttag tgccaaataa aatatcagca gaaagttttt
     6661 atagaaaaaa tatttttcaa aataaaaatg atttagatac aagaagtagt aatgattaca
     6721 ttggattact tcatggagat ataaacttat attcagagaa tgaagataat ggagaacatg
     6781 attttgtatt gacattaagg gatatagatt tgagtataaa cttttgcaaa ccttgtgtaa
     6841 ttgcaacagt tgatcagtta ttacttagta tgtttaaatt tccgggatat gaaaaaatat
     6901 tcgcagccgt aaaaaatgca tctataactg ttgatgaagt acatttattg gaaccaaaga
     6961 tgtttttgat tttgatatat tttatgcagt ttgcatgcaa gtacttagat gttgattttc
     7021 acttaatgac agctacactt ccaaaatcat ataaagagca aatgataaac aagggaatta
     7081 tttttcaaga agaaagtaat gaaaatgtta ctgataaagg agaaatagtt tttattgaga
     7141 gcaacaagga agaagagatt tgtgaaggta aagatgtaaa ggtttctttt ataaaggaaa
     7201 aagaaatcaa gtcaatagtt gaagatgctt tagaaaataa acaaaaaatt cttataataa
     7261 agaatactat tgattcagtc aatagaacat atgagttttt aaaagaaaat ttatctgata
     7321 agtatggtgg tgtagatata gatgttttac atagtagatt taagttcaaa gataaaaaag
     7381 agaaatatag taaaatatta aatggagagg gtgatatttg gatatcaact caatcagtag
     7441 aaatatcctt ggatttggat ttcaatataa taataagtga tttagcaact atggatagct
     7501 taatacaaag aatgggtaga tgcaatagaa ataataagta tgaatatgga aacttttata
     7561 ttctacctag tgaagataaa atttatgaca acaaacttaa gagcacaact aaaaacatct
     7621 taaaggacat attaaaaact aactctattt ttacaatggg aattagaaaa actattttgg
     7681 atgattacta tgataatagt gtagtcaaaa aatattttga agagaatttt ataagttgtg
     7741 ataaagaaat taagaatatt tatggtgtaa ataaagaaat ttttgatgga ttggatttaa
     7801 tttttaattt tgaaccttac aaaaatattg ttgatagtaa gaaggaagca gctaaaattt
     7861 ttagagatgt tgatgttagt tacaaaatta ttctggaaga agatttttat aaggaagata
     7921 gagagttaca acaagattcg attcaagtat cagggtttat atttaataga ttatattatt
     7981 atgggttaat aagcaaagta gaaggataca tggtactaaa atctagtagt aagtttgagt
     8041 ataatagtac tgtaggctta attttgaagt gagcatatgt ttaaatataa tataccttta
     8101 gacattgaaa atacaaaaag tactactgta agctcactgt aaaaactatt ttttaaaagg
     8161 aaaaataagt tgtgagatag agtgtttaca agtgtatata gataataaat ttgaggttta
     8221 agattaacta agtgggtttc aggtacatcg cgaaaataat tataagttgg agattttgta
     8281 tgtttaagat taactaagtg gatttcaaat attgatatag tagatataat agaagtttta
     8341 ttattaggtt taaaattaac taatcggatt tttaaatatt ctgactatta tataaggata
     8401 ttattagatt gaatttaaaa ccaataatat aaaacaacaa aatattatat ttatgatata
     8461 ataaaagtta tggaaaaaca ggaatattac tatagagaag agaggtaaac ttatgaataa
     8521 ttggatagaa gtaactataa aaacaacaac agaagcagtt gaacctataa cgaatatact
     8581 atatgaacaa ggagctggag gagctgtaat agaagaccca aaagactttt tattccaaaa
     8641 gaaaaatgaa ctagactggg attatgttga agaagaagta tttaaaaaga atgaagaaga
     8701 tgatgttctt ataaaaactt atgtttctga agaaaaaaat gtgatggaat ttgtcgaaat
     8761 aataaagcaa aaagttttag gattaaaaga ttttggtata gatataggtg aaggaagtgt
     8821 atcattggac caagtaaatg aagctgattg ggcaaatgca tggaaagcat actacaaacc
     8881 aacaaaagta ggacaaaggg tagtagtaaa gcctacatgg gaagattatg ctatgcaaga
     8941 gggggattta atcatagagc ttgaccctgg aatggcattt ggtacaggta cacatgagac
     9001 tactagtatg tgcattagag aattagaaaa gtatgtgaat aaagattcta aagtatttga
     9061 tattggatgt ggtagtggaa tccttgcaat agcagcagct aaattaggag caaaagaagt
     9121 tgtagctgta gatttagatg aagtggctgt aaaagttgca aaagaaaatg ttcttgaaaa
     9181 taaagttgaa aaaagtgtat ctgtaatgca tggtaattta actgatgtaa taaaagataa
     9241 agcggatgta atagttgcaa atataatagc tgatataata aagatacttg caaaagatgt
     9301 tcaaaacttt atgaaagaag atgctatatt catatcttca ggtataattt tggataaagt
     9361 agaagaagtt aaagaaagct taattgaaaa tggatttgaa atagttgaag ttcaaaaatt
     9421 aggtgaatgg tcagcaatag tttctaagct taaaaaataa gggatatggt gacgtataat
     9481 ggatagattt tttgttgaaa aaaataatat aaatctacaa gataaaactt gcactataga
     9541 aggtgaagat gtaaaacata tttcaaaagt tttaagatgc aaattaggtg agaaacttga
     9601 aatatgtgac aagaataata atgagtatat ttgtgagata atgaatatag ataagagtat
     9661 agttaattta gaaattttag aaaaagttga tataaataga gaatcagaac ttaaagtaag
     9721 gctataccag ggacttccaa aagctccaaa aatggaaata atacttcaaa aattaactga
     9781 agtgggtgta gaagaaataa tattagttca aactaagaga agtgttgtta aagttgatga
     9841 caaaaaagaa gataaaaaat ttgaacgttg ggaaagaata atttatgaag cagcaaagca
     9901 aagcaaaaga ggtaagatac caaaattaag aggtgtttta agttttaaag aagctttaga
     9961 agatatgaaa aaaaatagtg taaatatttg cccttatgaa aatgaaagaa cagtttcaat
    10021 aaaacatgca ctaaaaaaat gtgattctaa tatagatagt gttggaattt ttataggtcc
    10081 agaaggtgga ttttctgaag aagaaataga acaaatacaa aaaaataatt gtaatgttgt
    10141 atcattgggg cctagaatat taagaactga gacagcttct gttgtagcat caacaatagc
    10201 tttgtatgaa ctaagtgatt taggaggaga aaaataaatt gaaaaaagta gctttttata
    10261 cacttgggtg taaagttaat caatatgaaa cagaagctat gctagaacta tttgagaaag
    10321 atggttatga acaagtcaat agtgaagagt atgctgatgt ttatgttata aatacatgta
    10381 cagttactca tatgagtgat agaaaatctc gtcaatatat aagacgagtt aaaaagaaga
    10441 atccagatgc aataatagcc gttgtaggat gttattctca ggtatctccg gaagaaattt
    10501 tggatataga agaagtaaat ttagtcatgg gtacaaatga tagaagaaag attgttgaag
    10561 aaattaaaaa gataaattca agtaagaaag ttagtacagt tgatgacata atgaaagtta
    10621 aagcttttga agaaatagaa atcagccaaa ctaatggaaa gactagagca tttatgaaaa
    10681 tacaagatgg atgtgacaga ttttgtactt attgtatcat tccttatgcc cgaggacgcg
    10741 ttagaagtag agatattgat agtatagtcg atgaagttaa aaaattggct aataatggct
    10801 ataaagaggt tgtattaaca ggaatacatg ttgcctctta tggaaaagat ttaaaggata
    10861 gggatattaa acttcttgat gtaataaaac aaataaacca gatagaaaaa atagaaagaa
    10921 ttaggcttag ttctgttgaa ccaattttat ttacagatga atttgtaaat gaagttttaa
    10981 agatggataa agtttgtcct cattaccatt tatcacttca aagtggatgt gatgaaactc
    11041 taaaaagaat gaatagacga tacacaactt tagagtacaa aactattgta gatagattga
    11101 gaagtaaaat gccagatgtg gcaataacta cagatgtaat agtaggtttt cctggagaga
    11161 caaatgaaga gtttaaaaaa acgtacgagt ttttaaaaga aatagagctt tcacaaatgc
    11221 atatttttaa atattcacca agaaaaggaa ctccagctgc aactatggaa aatcaagttg
    11281 accctcaaat gaaacacttt agaagtgaac aacttttaaa tttaagtaaa gtaaatttta
    11341 ataaatttgc aactaaattt atcggccgtg agttggatgt tttatttgaa caaaatatag
    11401 aaggcaataa atatgaggga ttaacttcaa attatataag agttgttgtt gaaagtgaca
    11461 aaaatataca aggtcagata ttaaaagtga aaataaatga tgtaaaagat gaatatgtag
    11521 aaggaatttt gctataacat gtagaaatta taatatttat aagataggag gtggaaagaa
    11581 tggattgtat tttttgtaaa atagcgaacg gagaaatacc ttcaactaaa gtttatgaag
    11641 atgatagagt cttagctttt aatgacttga acccagttgc tccatatcat atattagttg
    11701 tacctaaaaa acattatgat agtttaattg atattcctga taaagagatg gacattgtgt
    11761 cacatattca tgttgttatt aataaaatag ctaaagagaa aggttttgac caaactggat
    11821 tcagagtcat aaataattgt ggaagtgatg gagggcaaga agttaaacat ttgcactatc
    11881 atattttagc tggtaaaaag cttcctaatt acgaagcggg acaaaactaa actaaattat
    11941 tgaaaaattg agttccttct tgataaaaat acttgataaa agtaaaaatt tagtttataa
    12001 taaacaaagt gttaggttgt aatgcatatt atgcaaaaag aacatagtta cagtcccagc
    12061 ataattgcgc tattggaggg agggaaaaag aaatgtcaga agtaagagtt agagagaatg
    12121 aaacattaga cagcgcttta agaagattca agcgtcaatg tgcaatgtct ggcatcatgt
    12181 cagaagtgag aaaaagagag cactatgata agccaagcgt taagcgtaag aaaaaagctg
    12241 aagcagcaag aagaaaaaac gctaagaaat agtagtaaat aagaataaag aggtgaaggg
    12301 gatgtccctt aagcaaaagt tacaagagga tttgaaatct tcgatgaaaa acaaagatac
    12361 agtgagaaaa tctgtagtaa ctttaataag agcttctata aagcagtatg aggttgataa
    12421 tagggtcgag cttgatgaag atggaattat cgatgtaata gcaaaacaat taaagcaacg
    12481 tagagatgca ttagttgaat ttgaaaaagc tggaagagaa gacttaataa aagaaacaga
    12541 aggtgaaatt gaagttttaa aagagtacct acctcaacaa ttaagcgaag aagagttaga
    12601 agaaattgta aaatctacta tatccgaagt aggggcaact tctatgaagg atatgggaaa
    12661 aataatgtca gttatccaac caaaagttaa aggaagagct gacggaaaac ttataaataa
    12721 gttggtaaaa caaaacttac aatagattga tatagcctag agagaaatct ctaggctttt
    12781 tatagttttt gggaataacg agtcatattt gagcatatct ataatcaaag aaaattttga
    12841 aagaagggga aaaatttgat tatatcagta aaagagatta ggaatttttt gataaacata
    12901 tttataccac taataatagg ttacttaagt agcatcttaa caatgattat cagtggaaca
    12961 gatatttcaa cctattattt acaactcaca aagccaagct ttgcaccacc agcttttatc
    13021 tttcctatag tttggactat attgtacata ttgatgggta tatcttcata cctaatatta
    13081 agaaagggtt acaacttacc taaagtaaga gatgcaattt tttattatgg attacaactt
    13141 gtattaaact ttatttggag tatactattt tttggatttg gacttagatt tacagctttg
    13201 atagatataa taataatgat tttggtttct ataataatga tttcgaagtt ttctaaaata
    13261 gataagagat ctggatacat aaatttgatt tatttaatgt ggcttgttta tgcaggattt
    13321 ttgaactatt ttatatggtt tattaataaa tagaatgtaa agattttgta aattaccctg
    13381 tacttttaat aatatcataa caaacgaaaa ataaatagac tattaaaaat aatcattttc
    13441 atatacaata atattaattt aaatacaata aatttataaa aattgtattt aaagattaac
    13501 atattaaagt attagaattg gaaaggcttg aagggagagg tttttatgca aataatagta
    13561 tctgggagac aaatgaaatt aactgacgga ataaaaggat acgtagatgg aaaattaagt
    13621 aggttagaaa aataccttga tcctgaatca gaagtgaaag taacagtaag tgcgaaaaag
    13681 gatagacaaa aagtggaggt tacaataatt cctattaacg gtcaaataat aagagcagaa
    13741 gatgtagaag atgacttata tgcagcaatt gatattgttt gtgacaaatt aagtagacaa
    13801 gttgtcaaat ataaaactaa agttaaagac aaagttcaaa ataataagag cataagattt
    13861 gaaaatcttg actttataga taatagcagt gaatttgatg attatgatta tgatgatgaa
    13921 gaagatgaaa atatagtaat agaaagaaga aagaaattta atgtaaaacc tatgagttct
    13981 gaggaggcga tacttcaaat ggaactagta ggacacaatt tctatatgtt tagaaaccaa
    14041 gataattttg aaataaacat agtgtataag cgtaaagctg gtggatatgg tttaatagaa
    14101 caagattaat aaaaattgta tacgtttaga ttttatataa gaaaaaataa aaaggtaatc
    14161 atgttgattt tttatcaata tgattacctt tttcttgttt aaataatata ttagatatta
    14221 ggtatttatt atataaaaat tttaaaacta atctgttaca acatcaataa agtgcataaa
    14281 tacctatttc aagcataaaa ttaattaagg gggtaatgct tatgttagaa atatccactg
    14341 atttacaagt aaatcaacca gttattactg ttacaagtaa tacgtttatt agtatagaaa
    14401 attatctatc aatattagaa tatgaagttg atttaataag aataaagact aaagttaaaa
    14461 caattaaaat atctggggat aaactttcac ttaagtatat tactgatagt gagataggta
    14521 taaaagggat tatctacaat gttgagtatg ttgattagga ggagatacac ttgataagtt
    14581 ttataagggg atactatgtt atagttgtgg aaggagtagg actcgaacaa tttttaaatc
    14641 atcttataag aaatgggata agtgtttaca atgtaactag aataaaaaat actaaaatgg
    14701 agtttcatat agatagacaa gatataaaag actttaagaa tgtttataga ggaagtaagt
    14761 ttgatataaa ggtaaagcaa aaaactgggg tcccttttat tatcaaaaga gtttataagc
    14821 acaaaggtat gtggatttgt gctcttgttt ctttattttt attgatgtct acatcacagt
    14881 ttgtgacaga tgtatatata caatctcctg aaggtataaa aaaagaagct cttagaaatg
    14941 agctttataa ggttggagta aggccaggag tctataagaa gagtattgat agaaaagaag
    15001 ttagagacca tatgatgtca aaatttaatg atgtggcata tttatctatc aatgtaaaag
    15061 gaacaaatat atttgttact gttacaaaaa aggcagaatc cctaaaatct acagaccaat
    15121 caaattattg taatgtaatt gctttaaaaa atggtataat agaaaaggta atccctagaa
    15181 gtggtaaatc agttattaaa tctggagaca tagtccaaaa gggagatgtc ttattaaatg
    15241 gagctaatac caagtcaata ccagaagtat gggcaagtac tttttatgag tccacaaaaa
    15301 aggcaagtta tgtagatact gtaaataaaa aaactggaga aaagaaaaat atatatacac
    15361 taagttttta tgataaagag tttacaatga gaaaaaatat aaaatataaa gattatgttg
    15421 ttgaaaataa agaaaaaaaa ctatcaatag gaaactatac ttttccaata aagataaaaa
    15481 cgagtacttt ttatgaaaca aaaaaggttg aagtaaaaag gaataaagaa gagttaaaaa
    15541 aagaattatc tgaaaaagct ttaaaagagt tagaatacat aatcccagct tcggctagaa
    15601 taatagatgt aaaacataac tttaaggtta ataaaaatat gttagaatat ttaataacag
    15661 ttcaaactag tgaaaatatt gctaaaattt atcctcttag taagtcagaa gcagaaaggt
    15721 ttataaagga agaaagtaaa ccagatgaag gtgaagagga agttccttcc aatccagaaa
    15781 aaagaccatt aaatgatata agaaatgagt ttgatgagga taataaggat aataaagacc
    15841 aaaacaattc tgatgaaaat aatagtaatc aaaatagtaa taatagtcaa aataataata
    15901 gcaataacaa ctaataaaat taaacttaaa atcttaggag gactaattca attgataata
    15961 caaaagaaat ttacaatagc agatggtaac tttgaaagag agttatttgg caactttgat
    16021 gaaaatgtaa aattaataga gaagacttta aatatagatg taatacttag agaaggcaat
    16081 ataattttaa ttggagaaga aaaaaatgta gattctgcat taaaacttat gaatgaatta
    16141 caccaaactg tatcaaatgg taagcattta gataagcaga gtatttctta ttcattatct
    16201 cttttgcttg aaggaagtga acaaaaaata aaagaacttg aggggacaat cgtcataact
    16261 caaaggggaa aagctgttca acctaaaact ttaggtcaaa aagaatatat aaaacttata
    16321 gaaaataatg atataacttt tggtgttgga cctgctggaa caggaaagac ttatcttgcg
    16381 gttgctatgg cagtaaaagc ttttaaaaga gatgaagtta gtagaattat acttacaaga
    16441 ccagctgtgg aagcaggaga gagtctaggt tttttaccag gagatttaaa ggacaaagtt
    16501 gacccatatt taagaccact atatgatgcc ttatttgaaa tgttgggtgc tgataaattt
    16561 aataagtatt tagaaagagg tactattgag gttgcaccac ttgcatttat gagaggtaga
    16621 acacttgata attcatttat aatattggat gaagctcaaa atactacttc tgaacaaatg
    16681 aaaatgtttt taactagact tggatttggc tctaaagctg tagttacagg agatgtaact
    16741 caaacagatt taccacaaaa taagaagagt ggtctaatac aagctacaga gattcttaaa
    16801 ggtgttccag gtattgggtc tataatgcta acagatagag atgttgtaag acatgagttg
    16861 gtacagagaa taataagagc ttatgagaag cacgacaaaa gagaagaatt taaaaaagaa
    16921 gaacgtaaaa aagcgaaaat gcaggaaaag actttcaaaa gaaagtaaaa gtgtagtaat
    16981 atagatatat agcattatta atgaataaaa agtaggtgta gtgaatggat ttgatactag
    17041 atgatagaca agataaatta gaagtaagtg aggagttaat tgaaaagata aaggatataa
    17101 tcatagaatg tttagattat gaaggatatg atgataatta tgaagtgagt ctttcttttg
    17161 tagataataa agagattcat gaattaaata gagaatatag aggagttgat agagtgactg
    17221 atgttttatc atttcctctt ttaagtgatg attttgaaga tgttgagtta gaagaagaat
    17281 ccctaggtga catagtagta tctcttgaaa gagccttaga acagagtata gagtataacc
    17341 atagttttga aagagaagta tgttttttaa tatgtcatag tatgtttcat cttttaggtt
    17401 atgaccacga tacagatgaa aatacaaaag aaatgagaga aaaagaagaa cacatattaa
    17461 ataagttaaa cataacaagg gagtagtttt tatgaaacca gaaaaaactc gtcaaggtat
    17521 aattaaagca tttaatgcag ctatagaagg gatattgtat acatttaaat ttgaaagaaa
    17581 tatgaaaata cattatttag gttcagttgc tgttcttata ataagtttgt tttttaactt
    17641 ttcaaaatta gaaatgataa tgttacttat gtccatatgt ttagttgtag ttgctgaaat
    17701 gtttaataca gctatagaaa aagctgttga tttagtaact gatgaatatc atgttcttgc
    17761 taagatagca aaagatgttg cagctggagg cgttctagtg gcagcactaa attcagtagt
    17821 agttggatat attttgtttt atgataaatt gacagatata tctggaatat taatttataa
    17881 aataagagaa tcagaattac atataacctt gatatgtata ttattggttc ttatagctgt
    17941 tgtagttgta aaagcattga cttctacagg tactcctcta aaaggaggaa tgccaagtgg
    18001 tcatgctgcc ttagcatttg ctatagcaac tgcaattaca cttatgactg aaagagttgt
    18061 tgcctctact cttgcatata taatggctgt attagttgcc caaagtagaa ttgaaggaaa
    18121 aatacataca ttttgggaga ctattgctgg agctttgtta ggtgtgttga tagctatatt
    18181 agtatttcag cttggaatgt tctataatta aaataaatgt acataaaata tttaaaataa
    18241 aacaaatctg tgctaattaa atttttatga tataatgtag agtattaaat aaaaataact
    18301 tttaattatt gtatacagga ggagttgttg tggataacat agaattgtta aggttggcag
    18361 aagatgccag acaacattct tatgctcctt actcaggttt tagagtaggt gcagctcttt
    18421 taactaagag tggtaaagtt tatactggat gtaatataga atgtgcatca ttaggtggaa
    18481 caaattgtgc agaaagaaca gctattttta aagcaatatc agagggtgat aaggatatct
    18541 ataaaatagc tatagcaagt gataactcag aaaataatga gcaaacatat ccatgtggaa
    18601 tatgcaggca ggtaattata gagtttggtt ctgatataaa aataataacg ggatatacta
    18661 aaggagaggt gtttgagcat agtataaagg atttgttgcc aaactatttc tctggaaatg
    18721 atttaaaata aatttaaaag tataaataaa tttaagtaat taaaacaaga agataaaaag
    18781 ttaggagaca gttaatgttc aaatcaggtt ttgtcagtat agtgggtaga ccaaatgtag
    18841 gaaaatctac tttaatgaat aatgtagttg gagaaaaaat agctataatg agtgataagc
    18901 ctcaaactac aagaaataca atacaagcag tatatacaga tgaagaaatg cagatagtat
    18961 tcttagatac accaggtatt cacaaaccaa aaaataaatt aggtgaattt atggtaaagg
    19021 cagcaacaga agcttttaaa aatgtagatt taatattatt tgtagttgat gattctaaaa
    19081 aaataggtcc tggagataga aagataatag aggatttaag aagtgttaag acaccaataa
    19141 ttttagttgt aaacaaaata gaccaactag accaaaaaga tgagctattt gatataatca
    19201 aaatgtatga tagagaaggt atttttaaag aaatagtacc aatatcagca ttaaaaggaa
    19261 agaatacgga taccttaata aaagttatac aaaattattt agaagaaggg ccaaagtatt
    19321 ttccagatta tatgattaca gaccagccag aaagagtatt aatagcagaa cttataagag
    19381 aaaaagttct tcactatctt aatgatgaaa tacctcatgg tgtagctgtt gaaattgaaa
    19441 aaatgaaagc gagaaatgat aaggaaatag tagatgtatc tgctgtaata tactgtgaaa
    19501 gagattcaca taagggaata ataataggaa aaaatggtag gaaactaaaa ggaataggta
    19561 agtcagcaag acaagatata gaattgcttt taggttcaca aataaatctt caattatggg
    19621 ttaaagtcaa ggaaaattgg agaaatttac aaaattatat aaataatttt ggatataatg
    19681 ataaataaag gtgggttata tggataggaa actggatgct tcccaagatt tattatatga
    19741 agtaaaatca ttaattgata ataataaagt attggagtta agagaattaa tagaggaata
    19801 tcatataatt gatatattcg atataatgga aaatttagag gaagacatga aaattcaatt
    19861 atttgaagta cttcctttag atatggcatc ttctatatta gaagaaggta gtgtagagtt
    19921 ttttattagt attttatcta aactggatgt agaacactcg aaaaatatac ttgaacttat
    19981 gtctcttgga gatatggcag acaagttgag tgaacttgaa gaagaagaaa gagaacacat
    20041 aataaatctt ttgaatcaag agaatgccga ttatgtaaaa gagttactat tctatgatga
    20101 agattctgct ggtggaacaa tgaccactgg atatatttct ataaataaag atatgacagc
    20161 tcttgaggct atagaccata tgagagaaga agctgaagaa gctgaaacta tttattatat
    20221 atatgtggtt gatgatgaag aaaaactagt tggagtattg tcattaagag aattaataat
    20281 agctagagac gcaaatatag tagaagattt gatgagtgaa aatataatct ctgtttatgt
    20341 agatgaagat agagaagaag cagtaagatt agtatctaaa tacaatttaa tagcaattcc
    20401 agtagtagac agacaagaaa aattaaaagg aataataaca gtagatgata taattgatgt
    20461 aatggaagaa gaagcaactg aagatatgta taaatttgca ggaagttcag aacatgaaag
    20521 agaagttgcg gaaaaagaaa accctacatt gagagagcaa ataatatcag cacttagagg
    20581 acgattacct tggttaatta ttaccttagt tggagggttg ttggcttcgt taatactttc
    20641 taacttagac tatataatga atcctgtata tgctccatta gtatttttta tacctgtagt
    20701 aattggtatg ggtggaaata ttggaactca atcatcatct gttacagtaa taacattgtc
    20761 taataaggat ttaaatttta gcaatgtggt tagagaaggt attgtaggta ttattacagg
    20821 tcttttgtgt agtataataa caggtattgt aatttacttt gttatgagaa agttagacat
    20881 tgtattaatt gtttcaatat cattatttat aaatatggtt ttaggtgcaa caataggtgc
    20941 gtttatgcct gttttattaa aaaaaatgga tgcagaccca tctacagttt catcaccaat
    21001 tatatctaca gctcttgata taactggtat agcagtttac tttataataa caacggcatt
    21061 attgtcaaaa attgtttaat aactttatgt atgatttatc ctcttcttac taaaaatata
    21121 agtataaatg taagaagggg gttttttaaa aatgaacaat atcagttggg aagtttttaa
    21181 aaaaacaggt agtatagatg catatttata tttctgtgat tgcaaaaaca tacatgaaga
    21241 aattgaagaa gtaagggaga aaaaagaaga cgatgataat cctgaacacc caggggatag
    21301 tacttaaagc tataaggtat aaggaaagtg atataatact tacactattt acaagaaagc
    21361 ttggaaaagt atctgcaata gcaaaaggag ctaaaaaaaa taagagctct ttgctttcat
    21421 catcacagtt attttcttat agcaatttta ccttaaaaaa acaaggtaat atgtacaagg
    21481 ttactcaaag tgaaataata aaaagttttt ataatatatc ctatgatata gaggcatttt
    21541 cttatgctac atatattaca aaattagttg aaaattctat acttgaaaat cagactaata
    21601 ataggctatt tatattactt gcgcagactc tgtatttgta tacacaggat aatacagaca
    21661 ataggtttat aacagcagca ttcgagttaa aatttttaga ctatatagga tttaaaccta
    21721 tagtaaataa atgcattaat tgtgaatgta aaattttaca aaattctgtg tttaatatct
    21781 acgaaggtgg tatactatgt agtaagtgta gtaaactttt tgataataat ataaaattag
    21841 acttaacaac tataagtctt atggaatatg tattaagaaa tgatatttta acatgtagta
    21901 aagctaaagt atcaaaatat ataacacatg aacttgagaa tattctagac aagtatttga
    21961 aagtttatgt tgataatatt aattttaaat cattacacgt attgcaaagt gtaaaaaata
    22021 ataagggagt ggataatgat gagtagtaat ataactattg aaatggttga ttcagtcttt
    22081 gaaagagttc caggagcaac ttatgcagaa gcaaaagaag cattagtaat gtgcaatgga
    22141 gatgtaattg aggcagtaat atatttagaa aataaaaaaa atacttgtaa ttgtaaagca
    22201 aaatctgcta aagaaactat ggaagaagta tttggcaaag atggagaaaa tataaagttt
    22261 caattaaaag agcttcttag aaaaagtagt gtagtgcgaa taatagttga aaaagatgga
    22321 aaaactatga tgaacattcc tttaacagtt ggagttgttg gattagcttt tgtaccatta
    22381 gctactttag ttggattatc agctgctgtt ataagtaaat atcgtataaa agttcaaaat
    22441 gaagaagatg gagaaattgt agatttaggt gaattgaatg aagaaaaact tcacgttttg
    22501 aagaatatga taactaatgc agcaaaagat gtgaaggatg tagtagttga caaaaaagaa
    22561 gatgacaaag atgtaactgc tgaccttatg aaagaagaat ctgaaaagaa taaagaagac
    22621 gaagacgaaa ataaatagat ataaagtgac ttttaaaaaa tgaagagaag ataattataa
    22681 gaattatcac taacattgac aaattttata gaatttgata ttctaaaagt tgtagaagaa
    22741 ctattaaaat agcctttcgt gtagacgaga ggctttattg ttataggagg gatttttatg
    22801 aattttcaag agatgatact ggcgttgcaa aaatactggt caaagcaagg ttgtatcatg
    22861 atgcagcctt atgatattga aaaaggtgca ggaactatga atcctaatac ttttttaaga
    22921 tcattaggac ctgagccttg gcaagtatgc tatgtagagc cttcaagaag accagcagat
    22981 ggaagatatg gtgaaaaccc aaacagacta tatcaacatc atcaattcca agttatacta
    23041 aaaccttctc cagataatat acaagaatta tatttggaaa gtttaaaaga gattggaatt
    23101 gacccaagtg agcatgacat aagatttgtt gaagataact gggaagctgc tacagttggt
    23161 gcttggggac ttggatggga agtttggcta gatggtatgg aaataactca atttacgtat
    23221 tttcaacaag taggtaatat tgaatgtgag cttgagacag gagaaattac atatggatta
    23281 gaaagattag ctatgtacat tcaagaagtt gatagtgttt atgatttaaa atggaatgat
    23341 aaaataactt atggtgaagt atttaataaa gctgagtatg aaaactcaat gtacgcattt
    23401 gaattatgtg atgcagatat gttgtttaat ctgtttgata tatatgaaaa agaagcatta
    23461 agacttatgg aaaatggcct tgtaatacct tcatatgact atgtgttaaa atgctctcat
    23521 gcatttaata cattggatgc aagaggtgca ataggagtta gtcaaagagc ttcatttata
    23581 ggtagagtta gaaatatggc aaagactgtt gcagaaactt ttgtaaaaca aagagaagaa
    23641 atgggattcc ctcttttaaa ggacggtgac aaataatgaa aaattatctt ttatttgaaa
    23701 tcggtgttga agaaatgcca tcaagatttg taggcagtac actagaacaa ttaaaaaaca
    23761 atcttagtaa attatttgat gaaaatagaa tagcttttga taaaataaat gcgtatggga
    23821 caccaagaag acttacttta gtagtcgaag gattgagtga tagacaaaat gatttagaag
    23881 aggaaataaa gggtcctgct aagaaaattg ctgttgatgc agaaaataat ttaacaaaac
    23941 cagcattagg atttataaaa agcaaaggat taagcgaatc tgacatatat tttaaacaag
    24001 ttggaaaaga agaatatata tttgcgacta taaagcaaga tggaaaagaa acttcagaag
    24061 tactaaaaga catattacca gagactataa aatctgtagt attcccaaaa gctatgcgtt
    24121 ggggaggaaa aaatcttaaa tttgcaagac ctataagatg gatagttgca ttgttaaatg
    24181 ataaagtatt agagtttgat ttagaaggaa taatatcttc taatgtaaca aaaggtcata
    24241 gatttttagg tgaaagtaca atagaagttg attctataga agattatttt gaaaaattag
    24301 aaaaaaatta tatagtttta gaccaaaaca agagaaaaga aatgataaga aaacaatgta
    24361 tagaagtggc aaattcttta ggtggagaag ttgaatttga tgaagattta cttgaagagg
    24421 taacttactt agttgaatat ccaactgctt tttatggaga atttgatgtg gattatataa
    24481 aactaccaaa agaagttgtt ataactccta tgaaacaaca tcaaagatat tttccagtac
    24541 ttaaagaagg aaaactgtta cctaacttta tagctgttag aaatggtgat tcttacagaa
    24601 tagacaatgt taaagctgga aatgaaaaag ttttagaagc aagacttgca gatgctttat
    24661 ttttctatag agaagatact aagagaaact tagaaagcta catagaaaaa ttaaaaactg
    24721 ttgtattcca agtaaaacta ggaactattt acgacaaaac tttaagactt gaaaaattaa
    24781 gtgctgatat acttgatagt ttaggtttag tagatgaaaa ggcagatact ataagggctg
    24841 ctaaattatc taaggcagat ttagttactg gaatggtagg agaatttgat gaattacaag
    24901 gttttatggg aaaagaatac gcaaaagttg gaggagaaaa tgatgttgta agtgaagcta
    24961 tttttgaaca ttatcttcca agatttgcag gagatatact tcctaagact aatgcaggag
    25021 ttgccttatc aatagctgat aagttggatt caatagcagg tttctttgct ataggaatac
    25081 aaccaacagg ttctcaagac ccatatgctt taagaagaca agctttaggt atacttagta
    25141 tacttatgga cagaaaagtt gctgtggata taaaagtact tattgaacat gctttagaca
    25201 attattctga attagatttt aataaagaag aagttgttga gcaaataatg aatttcttca
    25261 atgagagagt aaaaaatcta tttaaagatt taggaataag atatgatgtt gttgatgctg
    25321 ttttaagttc tgatataaat gatgtttctg atatgcatat gagagcttta gagcttaata
    25381 attggctaga aaaagatgaa ttagtggaga tgcttactgc atttaataga gtttctactc
    25441 tagctcaaaa agcagaaagt gataaaattg atgaatcttt attaaaagaa gatgctgaaa
    25501 ttaaacttta caatgaattt aaacaagtaa aggtaaaagt taatgaatta ttaaaggata
    25561 agaaatatgg tattgcttta gatgaatttg catctttaag accagtagtt gataatttat
    25621 ttgatacagt tatggttatg gataatgatg aagctattaa gaacaataga cttgctttac
    25681 ttaaacaagt atatgatact atgctaggaa tttgtgattt atctaaaatt gtttataaat
    25741 aaaatttaaa ctatttataa gaaataaggc tgtctcaaaa tagagatagt cttatttttc
    25801 attgttgaga tgatgaaaat atatttgatt tcatagaaaa agagtgtctc ataaactaaa
    25861 aagttcattt tgagacaccc atgctttttg taagtaaata taataaatta tattttatac
    25921 ttatcagtta ttttgtttaa aatattatca tttgaattta cccattcata aaaattgcct
    25981 gatgatgcgt ccgatatttt ataaagcatt tcaggaatat cactagcttt aaaatctcca
    26041 agtgattttc ccaatctagt cttacctatt tcaaaactgc ctccaacaaa taattcaaat
    26101 gcatctgtag atataccatc aacattcttc tttttaccac ataaacctat gctaccaact
    26161 tgatgaacac cacatgaatt taaacatcct gatatatata acttaggtgc tttatttaaa
    26221 atttctttat tattttgtaa tctaaaataa tctatgattt catgtaacat tttctgacta
    26281 ttttgtatgc ccatttgaca aattggaaca cctatgcaag atatactttg ttctaattga
    26341 gaattacaac taatagtttt acttatttct aatattcttt ttgcttcatt tccatttaaa
    26401 tttaaaatat ataatccctc tgtcattcct agtctaatca taggattttc aatattatct
    26461 aattctttaa gtaaagtact taaatccttt gttaacaaaa tacctccaac tggatgtata
    26521 tatacagtgt ataaaccatc ctgtttttga ggtattagtc tagaatcctg tatatctatt
    26581 ttaaccccag gtttagagta atctatttct tcaatattga gttcaagact accattttct
    26641 ttttctaatt tatagtattc tttaaatttt tctaagaata atttttcacc aagtgattcg
    26701 accatatatc taaccctagc tctgttttta ttttcataat ttccataatc cataaagaat
    26761 ttaatcatac cttcaacaca atataaggca tctttcggtt ttataagttc atcaagttct
    26821 aaagcaactt tgggttgttt acctagacct ccacctaaga aaactctgaa atatggttca
    26881 tcatttttca aagtagcaac aaatcctaag tcttgtatac tacaatgagc agtatctgtg
    26941 taacaattag aataagaaac ttttaatttt ctaggtaaat ggtatgtagt agcatttttg
    27001 ataaaatatt tatcagtagc aactgcatat ggagtaacat caaagacttc actagggtca
    27061 actccagata atggagataa tccgacattt ctaggataat taccaccacc acctctagtg
    27121 aatatatcat tttttattcc ttcttccatg atatcaacaa ttgaatttat agataaatcg
    27181 tgtaattgta tagcttgtct agttgttaag tgtatcttat ctaagttgtg tttaatagct
    27241 atttcataga ttttattaag ttgatactga gataaaactc cagcagatac tctaagtctt
    27301 atcataaaag attttttgtc tctttgagca taaacaccat atccaccaga aaatccctta
    27361 tattgcattt tatttatttc tccatttaaa aattttgaag aatattcttt aaaattaggt
    27421 agttcattta acaatatctt tttaaaatta tccattattt cacctcattg ttagtcttgt
    27481 ataaaaatat atctattgta taatagatat atatattatt atctagtcag gataaaaata
    27541 taacagaata attctattaa aagtaagaat aataaggaca tagtgagtat tttgttttta
    27601 ttttacaaat taagtataat atttttgttt tataaaatat aattaagata aatagataat
    27661 tttgtaattt taatatataa tatttagtag agtgtgggag tagcacttaa tatttgaagg
    27721 aggagttttt gtatatggga gaaataataa atgcattaga tagagcccta gacataattt
    27781 tgctgttata tcatgaaaag agagaaatgg gaataactga aataagcaag gctatgggag
    27841 tatataaaag tactgtacat agaactttgg ttactcttga aaacaaaggt tttgttatac
    27901 aaaatgcaga gaatggtaag tattggctag gaataaattt atatgcaata ggtatggttg
    27961 tgggagaaaa aatgtcattg actgaaatag taaaaccata tacgaaaaaa ttaaatcaag
    28021 aatttaacga agtagttaat gtatctatat tagaagaaag agctcaagat tctccaagaa
    28081 gtatcataat acataaagag tatggttcaa atcaattact ttcagtaaat ccatccgttg
    28141 gttcaagtag tgaatgttat tgttcagcag ttggtaaatg tcttatggca tttaatgatt
    28201 ctatagattt tgaaaaatat agaaaaacac caatacacaa gtatacagaa catacaattg
    28261 ataattggga tgatatgatg ctttttttag caaaaataaa agaacaagga tatgctatag
    28321 atgatgaaga actagaacat ggactaactt gtattggagc acctatctta gataaaaata
    28381 ataaagctat tgcagccata agtttgtcag gtccaactat aagaatgaga gaaggggact
    28441 ttgagtataa gattaaaaga gttatagaaa ctgcaaaaag tatatctgaa ctttttagat
    28501 gatttttaat ctcgataata aaaataggct atatttaaat tgttatttgt aaatatcagt
    28561 ttaaatgtag cctatttttc tttaatctaa aattaaatat tcttaaaatt taaaatgtta
    28621 ttgaaatata taagcgaatg tgttatcttt taattaagca ataaaaatag aacaaggttc
    28681 tatactatag aacaaaattg atgcatatat agtatttata caaaatataa atattatata
    28741 tttaaaatta agtaaaaata ttacataaag gggagatttg agaaatggaa aaagtcaagt
    28801 tgagtgttga aagatgtaag ggatgctatc tttgtattga agcttgtaaa aaagatgcaa
    28861 taagtctttc tgactacaca aataaaaaag gctttgtaac tattaaagtc gatgaagaaa
    28921 aatgtattca atgtggcgct tgttatacaa tgtgccctga tttagtattt gagatattat
    28981 aaattatttt tattagaggg gagaaaaggt atgcaaagtt tattaattaa aggaaatgaa
    29041 gctttagcag aaggtgctat acgtggtggc tgcagatttt atgcaggtta tccaataacg
    29101 ccacaatcag aaatattaga aatgatggca tggagacaaa aagaagttgg aggagtattt
    29161 atccaaggtg aatcagaaat agccagtgta aatatggtta tgggagcaag tgcacttgga
    29221 gcaagagcta tgacttcttc atctggtcca ggattttcat taaaacaaga aggtattgca
    29281 tattgggtat cagctggttt gccagcagtt gtagcatgtg tgcaaagata tggtataggt
    29341 gatggattta taggagctgg tcaagataat tattggcaag cagtaaaagg cggtggaaat
    29401 ggagattatc atttaatcgt gttagcacct aatagtgttc aagaaaatat ggatatggca
    29461 tatgaatctt ttgaattggc tgaaaaatat atgcatccag tacttatact ttctgatgct
    29521 acaataggtc aaatggtaga accttgtata gcaccaccaa tgaaagaata cgatatggat
    29581 aaagctccat gggctgcaaa aggaacacct gtaggagaaa aaaataaagt aataactgat
    29641 ataacatatt ttgaagacca agatacatgg caaaaagggt atatagaaaa aatgagaaaa
    29701 gttcaagaag atgaacaaag atgggaagag atagaaatag aagatgctga agtggtattt
    29761 gtggcatacg gtatttcatc aagaatagca gaaggtgcag ttagagatgc tagagcaaaa
    29821 ggaatgaaat taggtctaat aagaccaaaa acattgtggc cttacccaag aaaagcattt
    29881 aagaaattaa gtgattcatt gaagggactt gtaacagttg aaatgaacat gatggcacag
    29941 atgaaggaag atgtaattat agcatgtgat aatagatttc cagtatattc agtagcaaca
    30001 gctcaatata tggcagaagt aaatcaatta atagattttg ctcaacagat tatagatgga
    30061 aaagctaagc aagaggaggt attttaatta tggctaaaaa agcaccagca ataatgatgt
    30121 gtgaaaatat gttctgtgga ggctgtggac actctatagt cgatagaatt attggagaag
    30181 tattaacaga aatggatata tgtcaagata caattatatg ttcagatata ggttgtaatc
    30241 atatgtttca atttagtatg aatgtagatg ttgtcgttcc acctcatggt aaaatgggag
    30301 cagcaatggc agctatgaaa agagttagac cagataaata tatatttaca atagctggag
    30361 atggtggagc ttatgcaata gggcttggag aaactatgtc agctgctact agaaatgata
    30421 atgtaatatt tcttgttatg aacaataata tgtttgcaat gacaggaggg caaatggctc
    30481 caactactat agaaggtcaa tatacagctt ctactccagc gggtagaaat tacgcagatc
    30541 atggttcaac atttgatgtt gtaaaagtta tgggaaattt agatatagca tatctagcaa
    30601 gaggaacaat aactaaccct aaagaaataa atacaacaaa aagatacatc aaaaaagctc
    30661 tagaaaaaca aaaagaaaag aaaggttttt gcttaataga agtgcttgta ccatgtccaa
    30721 caaactgggg attaaagcca gtagatgcta tgaagagaat tgaaaatgaa cttgttccaa
    30781 agtatccact tggagaattt attgattagg gaggaaataa tatgttgaat gaaattatat
    30841 gtgctggttt tggaggacag ggagttctta cagcaggaaa aattataatg tatgttgcat
    30901 ataaggcaga aaagaaaatt acatggttcc catcttatgg aaatgagatg aggggaggaa
    30961 gtgcaaattg caatgttgtt gtaagtgatg aaaaaattgc aaatccttgt gtaaaaaatc
    31021 cagatatact attgggaatg aacagcgtat cagtagataa gtatgaaagt ttaatgaaaa
    31081 aaaatggtta tatgtttgtc aatgaatcac tagtaagtaa ggatagaaag tacagagaag
    31141 acattaatgt tataaaagta cctgttactg aaatagcaca aaatttaggc aatgaaaaag
    31201 ctgctaacat ttgcatgtta ggtgctatgg taaagagttt ggatttattt acaaaagaag
    31261 aatttgaaag tggtatgtgt gaatactttg aaaatcaagg caaaggtaaa ttcaattcta
    31321 aaaatgtaga agcatttgaa gctggatacg actacgaatg aatattttaa atatttaaaa
    31381 taattattga aatacttatt tttatatgtt agtatttaat taataaaata tagaacgtcg
    31441 ttctggacaa tgggacgaag tctttaggtc ggaatacaag aacggcgttc tgtataatag
    31501 aactaggggg tgttaaattg aaagttttag taataaatcc tggtggtaca tccacaaaaa
    31561 tttctgtttt ccaagatgaa aatgaaattt tgaaaaaaaa tataacccat acaagagaag
    31621 atttaaaaaa tttttctaaa gtatttgatg aatatgatta tagaaaacaa ctgatagtag
    31681 atattttaag tagtgagaat catagtataa attcttttga cgcagtagtt ggtcgtggtg
    31741 gacttatgaa ggctataaaa ggtggtacct atactgtgag tgaagaaatg attgaggata
    31801 tgagaaatga aataaatggg gaacatgcat caaatttagg tgcattatta gctaaaacta
    31861 tagctgatga aataggagta caatcatttg tagtagaccc agtttctgta gatgaatttg
    31921 atgatgtatc gaggattaca ggaatatcag acatagaaaa agcaagttgg ttacatgcat
    31981 taaaccataa agctgtttca agaaaaattg cacaaaaact agggggaaaa tacgaggatt
    32041 ataactttat tgtagctcat ttaggttcag gaatatcgat tgtagcacat aaacaaggca
    32101 aaatgattga tggaagtggt ggtaggtctg atggtccatt ttctccagaa aggagtggtg
    32161 gattacttgc atatcctctt atagaattgt gttactccgg aaaatatact catgatgaaa
    32221 tggtcgcaaa agtaagttcg attgggggaa tgtacgatta tttaggaaca aaggacatga
    32281 tagaaattga aaacagaatc aaggagggtg atgaaaaggc aaaacttata atggatgcat
    32341 ttatttatca agtagcaaaa gaaatttcta tgtatggagc ttcactttca gggaatgtag
    32401 atagagttat cttgacagga gggatatctc actcagaaat ggtagtcaaa ggagtagcag
    32461 aaaaagtttc atatctagct ccaatagaga tagtaccagg agagatggaa atggaggcac
    32521 ttgctctagg ggcacttaga gtacttaatg gagaagaaat agcaaaaact tattaatatt
    32581 taggagaaag agtatgttaa aagattttaa taatttatta gaagaggcta aaaagcttga
    32641 acctgttatt gtaagtgttg ctgttgcaga agacaaagaa gtattaaagg ctgtgaaatc
    32701 agcaacagat ttaaacatta taaagccaat tcttgtagga gacaaggata aaatagaaag
    32761 tatatcaaga gaaattggat taaaaggtca tgaaataata ggttgtcaaa atcccgatga
    32821 atcagttaaa aaagctgtag agatagttaa aaataaggaa gctaaagttc ttatgaaggg
    32881 attagtaaat acaagtgtct atatgagagc tatattaaat aaagaaacgg gtcttagaac
    32941 aggaagatta ttaagtttgc ttgcagtata tgaattacct caatatcata aacttcttta
    33001 ttgtactgat agtggaataa atgtatcgcc aaacttaaat caaaaaaaag atataatgac
    33061 aaatgcactt cttactatga aaagtatagg catggaaaat cctaaaactg cgatacttac
    33121 tgcaaatgaa atggtagacc ctaagataca atctacagtg gatgccaacc tacttgtaga
    33181 gatggaaaaa tcgggagaaa taccaaagtg tatagtggaa ggtccaatag catttgatgt
    33241 agcatttgat gcacatgcag cagaacataa aggtattaaa agtaatattg ctggtgatgt
    33301 tgatttatta gtatttccaa atatagaagc agggaatata ttaggaaagt catggcttag
    33361 atttaataaa gctaagtggg caggtatagt tttaggagca acaaatccga taattttagg
    33421 ttcacgttct gacacagctg aaataaaaat aaattcaata gcattagcgt gcctagcatc
    33481 acaataagaa attattatta aggaggaaaa agtattatga aatatgcaaa aagaatggat
    33541 atggtaaaag cttcagctat aagagattct caaaagaaaa tagctcagaa ggtagcaagt
    33601 ggtggaactg ttatatcttt tgctgcaggg cttcctgacc caaatttatt tccaatagaa
    33661 gagataagtg aaataactca aaaagtactt aatgaaaggg gaaaatttgc cctagcatat
    33721 ggacctacta aaggtgaaga tgagttgtta gatctattag taaaaagaat gaaagaagaa
    33781 gaaaatattg atacaaaagc agaaaatatt ataataacta ctgggtcaca acaagggata
    33841 gcattaagtg ctatgatttt attagaaaag ggagatgtag tagttacaga aaatccaagt
    33901 tacttaggtg caataaatgc atttagacct tatgaatgtg attttttagg agtagataca
    33961 gatgaagaag gaatggtaac agatgattta gacaaattat tatctgaaaa ttctaacata
    34021 aaggtgatat atgtaatacc aacatttcaa aaccctacag gaaaaacatg gtctttacaa
    34081 agaagaaaag attttatgga agtcgtgaat aaatatgatg tcatagtaat agaggataat
    34141 ccttatggag aaataagatt tacaccagaa cctctaccta cacttaaatc tcttgatact
    34201 aaagataaag tattgtacct tgggtcattt tcaaaggtat tatgtccagg atttagagtt
    34261 gcttggatgt gtggtaatgt agacatcata gataaggcag aacttttaaa acaaggtata
    34321 gatttacagt caaatcaatt ttcgcaagta caagtaatag aatttttaaa gaaatacaac
    34381 ttaaatgaac atatagaaaa aataagagca gagtataaga aaagatgtct gctaatgtta
    34441 gatgctatga agaaacattt cccagaggaa gctaaatata cagaaccaga tggaggaatg
    34501 tttatatggg ttgaacttcc tgaaagtata aatgtagatg atttattaga taaagccatt
    34561 gatgctggag ttgcttatgt ttctggagag tccttctttg caaataatgg tccaaaaaat
    34621 actatgagac ttaactatac aacaatgtca gaagaacaaa tcgttaaagg tgttgagata
    34681 cttgcagaag ttataaaaaa agaactcaat tatgcttgtg aataaatata aaaagtgagg
    34741 gtttaaatat gggagaaaaa gaaaacggta taaagtttgg aaagttaatg tttcaacttg
    34801 ttattatagc aatatcttgg gaattagttt atattatacc atttatccaa tatacattat
    34861 atgatccaat cctaaaagca ttagaatgta caaacactca actaggtttt ctacttacaa
    34921 tttatgggtt aggtaatata tttggagctc ctataggagg ctggttagca gatagatttg
    34981 attatagaaa gataatttta ggttctgtat ttcttaatgg agtagtatca tttttatttg
    35041 catttaatat gaactatcca tttgcagttg ctacttgggt aggttgtgca atcacatcat
    35101 tagttatgaa ctatccttct atggtaaaaa tacttagagt tataggaaaa gataatcaag
    35161 gtaaggttta tggatttaat gaagctatgg ttggagtttc tggagtagta atgggagcaa
    35221 tattcttata tatttataca tgctttgcaa ctcctacatt gggtatgaga tgggtaatga
    35281 tatctttagg aatattatca atagcaatgt gtccagtatt atggtttgta attaaagatg
    35341 tagatattaa agaagaaaga gaagaagcag gtgaaaaaat gtctgctggt gattttataa
    35401 cagtactaaa atcaccaaat acttggctag taggaataag tatattctgt gtatattcat
    35461 tcacagttac aatgtcttat tttacaccat atataacatc tgtacttgga ggttctgtag
    35521 cattatctgg tgcattagca ataataagac agcatggtct aaaattattt ggagcaccat
    35581 ttggaggata ttgtgcagac aaattaaaat ctcctacaaa agtactttta ccaatatatg
    35641 tatttggtat agcagtaata gttttattcc tagtattacc tgcatcaact ccaatgacta
    35701 tattcatagc tttaacattt gtagtaggta tattaggtta catgggaaaa ggtatatatt
    35761 atgcagtaca agatgaagta aaagttccag ttaaatattc agcaactact ataggtatag
    35821 ctgcagcttt aggattttca ccagatgtat tccaatttgc attaatagga cactggatag
    35881 atacttatgg caataaaggt tacacatata catttatatt ccaaatagca attctagtta
    35941 ttggaatact atcttgttta tatatattaa aggttaaaaa gagaagagca agtaaattag
    36001 aagcttaaat attcaatttg gtaattaagt atatataaat acaatactta ttttgatgaa
    36061 tgtgattgta taaataacat aaaaaatatc aatagattaa gttttataat aatgtttaga
    36121 gaataaagta taaaaatcaa aatgttatca agttttctag gttttaacat aaagttaccc
    36181 ctgaaggtgt aaaattgagt ggcaattatt atctattttc ttacctacat aaacctattt
    36241 aatttaaatt ctaagagatt ttcaaaatag atggtagttg tactcaattt taaacctcat
    36301 aataaatacg aaaatttaaa atctaactag atactattta attata
//
LOCUS       DAYATN010000022        36176 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.022, whole genome shotgun sequence.
ACCESSION   DAYATN010000022 DAYATN010000000
VERSION     DAYATN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.022"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            292..1875
                     /gene="asnB"
                     /locus_tag="ACGI8A_001126"
     CDS             292..1875
                     /gene="asnB"
                     /locus_tag="ACGI8A_001126"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFH0179132.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(1940..2536)
                     /locus_tag="ACGI8A_001127"
     CDS             complement(1940..2536)
                     /locus_tag="ACGI8A_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HFH0179133.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(2563..3090)
                     /locus_tag="ACGI8A_001128"
     CDS             complement(2563..3090)
                     /locus_tag="ACGI8A_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HFH0179134.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            complement(3452..4450)
                     /locus_tag="ACGI8A_001129"
     CDS             complement(3452..4450)
                     /locus_tag="ACGI8A_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFH0179135.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            5126..6325
                     /gene="hadA"
                     /locus_tag="ACGI8A_001130"
     CDS             5126..6325
                     /gene="hadA"
                     /locus_tag="ACGI8A_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HFH0179136.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            6364..7164
                     /gene="hadI"
                     /locus_tag="ACGI8A_001131"
     CDS             6364..7164
                     /gene="hadI"
                     /locus_tag="ACGI8A_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HFH0179137.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            7179..8405
                     /gene="hadB"
                     /locus_tag="ACGI8A_001132"
     CDS             7179..8405
                     /gene="hadB"
                     /locus_tag="ACGI8A_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HFH0179138.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            8405..9532
                     /gene="hadC"
                     /locus_tag="ACGI8A_001133"
     CDS             8405..9532
                     /gene="hadC"
                     /locus_tag="ACGI8A_001133"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HFH0179139.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            9580..10713
                     /gene="acdB"
                     /locus_tag="ACGI8A_001134"
     CDS             9580..10713
                     /gene="acdB"
                     /locus_tag="ACGI8A_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative isocaproyl-CoA dehydrogenase AcdB"
                     /protein_id="HFH0179140.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            10746..11531
                     /gene="etfB"
                     /locus_tag="ACGI8A_001135"
     CDS             10746..11531
                     /gene="etfB"
                     /locus_tag="ACGI8A_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HFH0179141.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            11550..12587
                     /locus_tag="ACGI8A_001136"
     CDS             11550..12587
                     /locus_tag="ACGI8A_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFH0179142.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            13070..14701
                     /locus_tag="ACGI8A_001137"
     CDS             13070..14701
                     /locus_tag="ACGI8A_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0179143.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(14836..15762)
                     /gene="fba"
                     /locus_tag="ACGI8A_001138"
     CDS             complement(14836..15762)
                     /gene="fba"
                     /locus_tag="ACGI8A_001138"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HFH0179144.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            16092..16421
                     /locus_tag="ACGI8A_001139"
     CDS             16092..16421
                     /locus_tag="ACGI8A_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HFH0179145.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            16421..17143
                     /locus_tag="ACGI8A_001140"
     CDS             16421..17143
                     /locus_tag="ACGI8A_001140"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFH0179146.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            17170..18531
                     /gene="rlmD"
                     /locus_tag="ACGI8A_001141"
     CDS             17170..18531
                     /gene="rlmD"
                     /locus_tag="ACGI8A_001141"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFH0179147.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
     gene            18923..20992
                     /locus_tag="ACGI8A_001142"
     CDS             18923..20992
                     /locus_tag="ACGI8A_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0179148.1"
                     /translation="MQLTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSAL
                     MKLFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFF
                     KNMVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYE
                     LDQIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEE
                     KGVESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVE
                     ELGDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHIS
                     PHLLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSII
                     LPNEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEI
                     SIVPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNV
                     LLYTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKD
                     KYISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGS
                     TYTEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMID
                     DSGGGRK"
     gene            20989..22854
                     /locus_tag="ACGI8A_001143"
     CDS             20989..22854
                     /locus_tag="ACGI8A_001143"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFH0179149.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            22978..23172
                     /locus_tag="ACGI8A_001144"
     CDS             22978..23172
                     /locus_tag="ACGI8A_001144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179150.1"
                     /translation="MKKKTKISKKIVSFIMVFITIVTTILTSSVSAYASELDMSEQTG
                     FNYLGISPITGKKLIITFIV"
     gene            23175..23792
                     /locus_tag="ACGI8A_001145"
     CDS             23175..23792
                     /locus_tag="ACGI8A_001145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179151.1"
                     /translation="MDNKIVFCVEPGIKTSSGGGYISEELGDKFISTTLPNYHKRKAE
                     ILEKVNRHNILPSWNNEKVAVKSGETIELEDKNGIIREMQLESNNTGTDILLEENTLK
                     ITANKNSNSGVISYIKIPKNEVGISIVYRKHNYQSLSEFHMESSMSANLNINVIKLGI
                     VKVKKIDEGTGKELANAKLRFEYDGDSKEIITGSNEIASIEDIIG"
     gene            23784..24305
                     /locus_tag="ACGI8A_001146"
                     /pseudo
     CDS             23784..24305
                     /locus_tag="ACGI8A_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901831.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            24418..24579
                     /locus_tag="ACGI8A_001147"
     CDS             24418..24579
                     /locus_tag="ACGI8A_001147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179152.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            24819..25046
                     /locus_tag="ACGI8A_001148"
     CDS             24819..25046
                     /locus_tag="ACGI8A_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0179153.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            25043..27982
                     /locus_tag="ACGI8A_001149"
     CDS             25043..27982
                     /locus_tag="ACGI8A_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFH0179154.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            27982..28656
                     /locus_tag="ACGI8A_001150"
     CDS             27982..28656
                     /locus_tag="ACGI8A_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179155.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            28817..29233
                     /locus_tag="ACGI8A_001151"
                     /pseudo
     CDS             28817..29233
                     /locus_tag="ACGI8A_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            29193..31640
                     /locus_tag="ACGI8A_001152"
                     /pseudo
     CDS             29193..31640
                     /locus_tag="ACGI8A_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            31780..32202
                     /locus_tag="ACGI8A_001153"
     CDS             31780..32202
                     /locus_tag="ACGI8A_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179156.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            32740..33774
                     /locus_tag="ACGI8A_001154"
     CDS             32740..33774
                     /locus_tag="ACGI8A_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HFH0179157.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            33861..34064
                     /locus_tag="ACGI8A_001155"
     CDS             33861..34064
                     /locus_tag="ACGI8A_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFH0179158.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            34235..35395
                     /locus_tag="ACGI8A_001156"
     CDS             34235..35395
                     /locus_tag="ACGI8A_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HFH0179159.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            35654..35794
                     /locus_tag="ACGI8A_001157"
     CDS             35654..35794
                     /locus_tag="ACGI8A_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179160.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT        14321 a         3981 c         6445 g        11429 t
ORIGIN      
        1 ctttataaaa tcttcagaat ttcttaaaga atatgtaagt aacataagat atattatatt
       61 tagttggtaa tataaagggg gcaatattta tgaagaataa aaaattcaat gttataacac
      121 tgataatact tgataatcat atttatgcca ttaataaaga gcagattagt actaattcaa
      181 aaagaattta ctttgaaaaa agtcgatttt tcagtgatta taagtgaata aatatatata
      241 aacatttaat aaatttagaa gttaaatgtt ttaatacgaa aggatacaca tatgataaat
      301 ttagaagaaa agtatttaac acgaaaagac acatatatga taaaaattct agaatacaat
      361 gggcatattc gaaactggaa agttctttgt gaagaacttg gaatcgattc ttcattatgt
      421 agagatgagc gtgaacgagc aattttagtg gaagcatata agacatgggg atatgatatg
      481 gcaaatcaca tgcatggaat gtttgctttt gcattatggg atactgtgga aaagaaactc
      541 ttctgcctaa gagaccagtt tggggtaaaa cctttctatt attatgagac tgaaaatggg
      601 aagcttctct atggttcatc tattcgtaag attatggagc aaccaggatt tgtgaaagag
      661 ttaaatgaag acatgttaca gatttatatg tctttcacat atgtagcagg tgaaaataca
      721 ttcttccgtg gagtaaagaa gcttatgcca ggacattacc ttgtctatca gaataaaaca
      781 gtatgtattg gtcgttattg gaaacctgaa tttcatcctg accacagcaa gtctattgag
      841 gaatggacgg atgaaattca tacaacactt cagaaaatta tgccagaggt aaaatcagag
      901 aatgaaactg cagagttatt cctatcagga ggtgttgatt catcttatat attggcaatg
      961 tcagatatag aaaaaacagg ttcatgtggt tatgaggaag agcgttttga tgaatcaaaa
     1021 ttagcacaac agacagctaa tattcttggg tgtaaaaatt cgagatatat aattacacct
     1081 gaagagtatt ttgcgtcagt accttatgta atgtaccata tggagcaacc aactggagat
     1141 gcatctgcta tcgtttttgc tattggatgc aaggcagcag ctgaaaacac aaatatctgt
     1201 tactctggag aaggagcaga tgagtttttt ggtggatata atatatacca taaggcagag
     1261 tgttatggag agaatttgaa gacattttat attggaaata caaacattat gaatgagggt
     1321 gagaaaagaa aaattcttaa gaaatacaat cctgatgtgc ttcctattga agtggtaaaa
     1381 ggaatttatg aagaaacaga agaactagat acacttacta acatgtcaaa tgtagatatt
     1441 caaatatggt tggaaggaga tatttatttt aatatagata aaatgagtac tgcagtagga
     1501 ttggaagtca gaatgcctct tacagataca agaatatttg atattgcatc cagaatacca
     1561 tcagaatata aagtgaaagg aaaagagaat aaaattgcct ttcgtacagc tgcatcaaaa
     1621 acattaccag aagagattgc ttttagaaat aaattaggat ttattgtgcc aattcgtata
     1681 tggatggctg atgaccgata taataaggat atccgagata aattcaatag tgagatagca
     1741 gataaattct ttaacataga tgagattaat gagattttta atgactacat tagtggaaac
     1801 tctgataatt ggagaaagat ttggatgatt tacacattcc tagtgtggta tgaaatctat
     1861 tttgtaaaat gttaatacaa gaggaagaat cacctgaaat accattcagg tgattcttaa
     1921 tgttttataa ttttacttgc taattcttat gtctaactct taatggtata tcaagttttt
     1981 ttgccagttg tttagattct tctatttctt ctggagatat ttcatctact actgaaaaag
     2041 ctacttcttt tatatatttt ttgcaatctc ttgcaaaatc taacatataa tcaaatgatt
     2101 tttctccaaa ctttggttga gtaactctat tataagcttc tttatttggg gcattaagac
     2161 ttattgatac tgcatctata acatttttta acagaatagc tgtttttttg ttatgtatta
     2221 aatcacttag accatttgta tttattctta tctttatgct acttttttct tttataaact
     2281 ttgctatctc tatcaattca tttattctca ttaatggttc gccatatcca caaaatacga
     2341 tttcatcata gtcgtctaaa ttgtatttat tgaaggcttc ttttacttca tctacacttg
     2401 gctcatgctc caaccataaa ctaccactat ttgcaacttc atctttttcg tttcttatac
     2461 aaaaaacaca actacatgga catgtattag ttatatttac atagatacta ttttcaattg
     2521 tatataagat attcataatt ttccctccat aaatttaaat tcttaattaa aataacgttt
     2581 tttaaaatca agtttatcaa gtgccatacc taagattata aaaacaataa aacttcctat
     2641 tggctgtaat aagccaagta agctcattac aaatgcactt gtaaaacttc caataattat
     2701 accttcagca aacatgtata aaactaatcc tactatacct gctacaaccg aagcaactat
     2761 atttctctta tttattatct tgtcactttt agaagtgaaa aataatacaa gtattggttt
     2821 tatgattata gttggtatta cccagatagc agcacctgta gataaatcag ctagaccagc
     2881 accaattgca gaagcagcta tagcatagtt tgttggcaaa atagttgcag ctagataaat
     2941 aaaagcatct cctatatgaa catatccatt gtttccacca actggaatat gtaatatata
     3001 tgctattgtc aggcatatta tggatgcaaa taatgctgaa gtaacaatat aggtagtttt
     3061 tttagaagta actttattca ttttttccaa tatactccct ccctaaaaat ccagagctaa
     3121 cataattgtt atgttttctt ataaatatat taatattaat catacaattt tcatagtttg
     3181 ttaggtgttt tttttgtaaa atacagattg tacccattca aatgtatata attagaaaag
     3241 taaaaatagg taaaatgtat agagtaggag tagttagaat aagttaatat aaatttaatt
     3301 tggtatgaat aagaagagta caagtttaat ataaaaaata atataagaga gtctactcaa
     3361 tatatactga gctgactctc ttatatttgg gaaattaaag gctattttgt tagataatag
     3421 ctaattaatg aaaaaatata tttataaaat actaatttac tctattagta gcagttcctg
     3481 tttttataac ttctacagca ctacttaaac tagaataaac tatattttca atcgctgtac
     3541 tagtgaagaa accaaggtga tgagtgaata taacttgttc catagaaaga agtttattta
     3601 tttctgggtc agttaattct ccaggattca tttttttgtt taagaacaat ccttcagttt
     3661 caaatgtatc aagggcagca gctctaattt ttcctgactc tagtgcttct attaaatccc
     3721 ctgtattaat taaaccacca cggccagtat ttactatgta agctccatcc ttcattatag
     3781 ctagagtatc tttatttatc atatgttttg ttccttcaag taaaggagta tgtaatgtta
     3841 taaggtcagc ttcttttaga aggtcttcta aagaatcttt gtaagttaat atatcattta
     3901 aatcactatt tggatattgg tcaaatgcaa ttacattagc tcctaggcct ttgaataatt
     3961 ttgcagaagt agcacctatt tttcctgtac ctataacacc aactgtaata gatctaactt
     4021 ctgttccaag aagttcagct atccaacggt aatcaccttc acagactttt ttctttacta
     4081 gaggagtctt tcttaaaagg ttcatagctt gagtaacagc taattcagct attgcattag
     4141 gtgaataaga aggaacgttt gtaacgataa gtccattttc attaactaaa tcaaaatgta
     4201 tcatgtcaac ccctgcagtt cttgaagcta taactttaac cccagcatct tttaaagttt
     4261 cataaatata aggattatct atatagttag tttgttgtat agatatacca tcataccctt
     4321 tagctttaac tacattttct tcagtcatgt tttcaggata aatatccact tgaacatcct
     4381 tatgttcttc agaccatttt tttattactg gttcttcata atcgcgtgct ccaaatacta
     4441 gtattttcat atcaataccc tcctgtaatt attaaattat tatggtaatt tttcatttag
     4501 aaataatatg tagcatataa ggttatattt tttatacaaa atttttaagg ttaaaaattt
     4561 agttttgaaa ttgttttccc tgtgttattt tgatatttag taatttttta tttaatgtaa
     4621 taattactct aaaatttatc aaagaaattg ttatttttta gattaaatat tattaaaaaa
     4681 ttatctaagt ttgaaattag ttaactaact tgctgattaa ttacaatgat aacatgtgtt
     4741 tttgagttta gcaatgaaaa ctttttttga aatgtcaagc tttttcataa aaaaatatat
     4801 aaaaaaactt ttataagcat agaaagaata gcaaaatgca atggatagtg gcaagatgcc
     4861 acgatatgaa aaagattatt caattgcata ttgttaaaaa acattcaaat caagcgatat
     4921 tttgattaaa tataaagttg gcacgataaa tgcttaacaa tataggtgta agacaataaa
     4981 acaataaaaa ataattttaa taatttttaa gttattttag ataagattag gaactttgac
     5041 atcagacagt taatatcaga aaaaagtatt ttatgagtga tttaatcgaa attttataat
     5101 tgatactatt aaggaggaaa acgttatgct tttagaagga gttaaagtag tagaactttc
     5161 aagtttcatc gcagcaccat gttgtgcaaa aatgttaggt gactggggtg cagaggttat
     5221 taagattgaa cctatagaag gtgatggaat aagagttatg ggtggaacat ttaaatctcc
     5281 agcatcagat gatgaaaacc ctatgtttga attagaaaat ggaaataaaa agggtgtaag
     5341 tattaatgta aaatcaaaag aaggagtaga aatattacat aaattattat cagaagcaga
     5401 catatttgta actaatgtta gagttcaagc attagaaaaa atgggtatag cttatgacca
     5461 aataaaagat aagtatccag gattaatatt ctctcaaata ttaggatatg gtgaaaaagg
     5521 acctttaaaa gataaaccag gatttgacta tactgcatac ttcgcaagag gaggagttag
     5581 ccaatctgtt atggaaaaag gaacatctcc agcaaataca gcagcaggat ttggtgacca
     5641 ctatgcaggt ctagcactag cagcaggaag tttagcagca ttacataaaa aagctcaaac
     5701 tggtaaaggt gagagagtaa cagtaagtct tttccataca gctatatatg gaatgggaac
     5761 aatgataaca acagcacaat acggaaatga aatgccttta tcaagagaaa atccaaacag
     5821 cccattaatg actacatata aatgtaaaga tggaagatgg attcaattag ctttaataca
     5881 atacaacaag tggttaggca aattctgtaa ggttataaat agagaatata tattagaaga
     5941 tgatagatat aataacatag attcaatggt taatcatgtt gaagatttag ttaagatagt
     6001 tggagaagct atgttagaaa aaacattaga cgagtggtca gctttattag aagaagcaga
     6061 cttaccattt gaaaaaattc aaagctgtga agatttatta gatgacgaac aagcttgggc
     6121 aaatgacttc ttatttaaga aaacatacga tagcggaaat acaggtgtct tagttaatac
     6181 tccagttatg tttagaaatg aaggaattaa agaatataca ccagcaccaa aagtaggtca
     6241 acatactgta gaagtattaa aatctttagg ctacgatgaa gagaaaataa ataactttaa
     6301 agatagtaaa gttgtaagat attaattagt aattctagat tttttaaaag gagaatgcta
     6361 aagatgtaca caatgggatt agatataggt tcaactgcat caaagggagt aatcttaaag
     6421 aatggggaag atattgtagc ttctgaaaca atatcctctg gtactgggac tactggacca
     6481 tcaagagttt tagaaaaatt atatggcaag acaggtcttg caagagaaga tattaaaaaa
     6541 gttgtagtta caggatatgg aagaatgaac tattcagatg ctgataagca aataagtgaa
     6601 ttaagctgtc atgctagagg ggtaaatttc ataattccag agacaagaac cattattgac
     6661 ataggtggtc aagatgcaaa ggtattaaaa ttagataata atggaagact attaaacttt
     6721 cttatgaatg acaaatgtgc tgcaggtaca ggaagatttt tagatgtaat ggcaaaaata
     6781 atagaggttg atgtatctga actcggaagt atatctatga attctcaaaa tgaagtatca
     6841 ataagcagta catgtacagt atttgcagag tctgaggtta tatcacattt atctgaaaat
     6901 gcaaaaattg aagatatagt ggcaggtatt catacttcag tagcaaagag agtttctagc
     6961 ctagtaaaaa gaataggagt acaaagaaat gtagttatgg ttggtggagt tgctagaaat
     7021 agtggtattg taagagctat ggcaagagaa atcaacacag aaattattgt acctgatata
     7081 cctcaattaa ctggtgcttt aggagcagcg ttatatgctt ttgatgaagc aaaagaatca
     7141 caaaaagaag tgaaaaatat ataaaaagag ggatgaaaat gtctgaaaaa aaagaagcta
     7201 gagtagtaat taatgattta ttagctgaac aatatgcaaa tgcatttaaa gctaaagaag
     7261 aaggaagacc tgtaggttgg tcaacatcag tatttcctca agagttagca gaagtatttg
     7321 acttaaacgt attatatcca gaaaaccaag cagctggagt agcagctaaa aaaggttctt
     7381 tagaattatg tgaaatagct gaatctaaag gatattctat tgacctatgt gcatatgcaa
     7441 gaacaaattt tggtctttta gaaaatggtg gatgtgaagc tttggatatg ccagctccag
     7501 atttcctact ttgctgtaac aatatatgta accaagttat aaaatggtat gaaaatattt
     7561 caagagaatt agatatacct ttaataatga ttgatacaac tttcaataat gaagacgaag
     7621 ttactcaatc aagaatagat tatattaaag ctcaatttga agaagctata aaacaactag
     7681 aaattatatc aggaaagaaa tttgacccta agaagtttga agaagtaatg aaaatatcag
     7741 ctgaaaacgg aagactatgg aagtattcta tgagtttacc agcagattct tctccttctc
     7801 caatgaatgg atttgactta tttacttaca tggctgtaat agtttgtgct agaggtaaaa
     7861 aagaaactac agaagcattt aagttactta tagaagaatt agaggacaac atgaaaactg
     7921 gtaaatcttc tttcagaggg gaagaaaaat acagaataat gatggaaggt ataccttgtt
     7981 ggccatatat aggatacaag atgaaaacat tagctaaatt tggagttaac atgacaggta
     8041 gtgtttaccc acatgcttgg gcattacaat atgaagttaa tgatttagat ggaatggcag
     8101 tagcatatag tactatgttt aacaatgtaa acctagaccg tatgacaaaa tatagagttg
     8161 attctttagt agagggtaaa tgtgatggag cattctatca tatgaacaga agctgtaaac
     8221 ttatgagttt aatacaatat gaaatgcaaa gaagagcagc tgaagaaact ggattaccat
     8281 atgctggatt tgatggtgac caagcagacc ctagagcttt cactaatgct caatttgaaa
     8341 caagaattca aggtttagtt gaagtaatgg aagaaagaaa aaaacttaat agaggtgaga
     8401 tataatggaa gctattttat ctaaaatgaa agaagtagtt gaaaatccaa atgcggctgt
     8461 aaaaaaatat aaaagtgaaa ctggtaaaaa agctataggt tgtttcccag tttattgccc
     8521 agaagaaatt atacatgcag ctggaatgct tccagttggt atatggggag ggcaaacaga
     8581 attagattta gctaaacaat atttccctgc atttgcatgt tcaataatgc aatcatgttt
     8641 agaatatgga ttaaaaggtg cttatgatga attatctgga gttattatac caggtatgtg
     8701 tgatacacta atttgtttag gacaaaactg gaaatcagca gtacctcata taaaatatat
     8761 atcattagta cacccacaaa atagaaaact tgaagctggt gtaaaatact taatcagtga
     8821 gtacaaaggc gtaaaaagag aacttgaaga aatttgtgga tatgaaatag aagaagcaaa
     8881 aattcatgaa agtatagaag tttacaatga acatagaaaa actatgagag actttgttga
     8941 agtagcttat aaacattcta atactataaa accatcaata agaagcttag taattaagag
     9001 tgggttcttt atgagaaaag aagaacatac tgagctagtg aaagatttaa tagcaaaatt
     9061 aaatgctatg ccagaagaag tctgttctgg aaagaaagtt ttattaacag gtatattagc
     9121 tgattctaaa gatatattag acattttaga agacaacaat atatcagttg tagctgacga
     9181 cttagcacaa gaaacaagac aattcagaac agatgtacca gcaggtgatg atgcgttaga
     9241 aagattagca agacaatggt caaacataga aggatgttca ttagcttatg accctaagaa
     9301 aaaacgtggg tcacttatag tagatgaagt taaaaagaaa gatatagatg gtgttatctt
     9361 ctgtatgatg aaattctgtg acccagaaga atacgattat cctttagtta gaaaagatat
     9421 agaagatagt ggaataccta ctttatatgt tgaaatcgac caacaaactc agaataatga
     9481 acaagccaga actcgtattc aaacttttgc tgagatgatg agtttagcgt aagttcatta
     9541 aaataaattt actataaaat atgaaaattg gaggaagcta tgttatataa caaagaacaa
     9601 gaacttttaa gaaaagcagt aagagatttt gttagtaaag aattagatac tttaccagca
     9661 gaaatggata aaacaggtgt aatgcctaag gaattaatca aaaaattagc tgatgcaaaa
     9721 tttataagca gtaatatccc agaagaatat ggtggtgggg gagcaggata tgtttcttac
     9781 gctatagtaa tggaagaaat agctagaaga tgtgcttcaa cagctacttt tgttacagct
     9841 ggttcttctc ttgcttcatt accaatactt tacaatggta ctgaagagca aaaacaaaaa
     9901 tacttaaaag gtatagcaac aggtgaatta ataggagcat ttggtttaac tgagccagga
     9961 gctggttctg atgcaggtgg acaacaaaca acagcagaat tagttggaga ccactatata
    10021 ttaaatggta gaaaaacttt cataactaat ggaccattct gtgatgtagc tatagtaata
    10081 gcagttacag atagaagtaa aggacttaga ggaacatcag cgtttatagt agaaagtaaa
    10141 tgggatggtt tctcaacagg agctcatgaa gataagatgg gtataagagg aactgaaact
    10201 tctgacttaa tatttgaaaa cgttaaagtt ccaaaagaaa acttacttgg aaaagaaggt
    10261 caaggattta agatagcaat gggtactcta gaagttggta gaataggtgt tgctgcctta
    10321 gctctaggaa tagctcaagg tgctttagat gaagctgtta aatatacaaa acaaagagtt
    10381 caatttggta agcctatagc taaattccaa aatactcaat ttactatagc tgacatggaa
    10441 acaaaagttt gtgcagctag aggattagtt tatgatgcag cacaaaagag agatgcagga
    10501 atgagagttg ctcaagaatc tgctatggct aaatactatg catcagaaat tgcaaatgaa
    10561 gttgcttata aagcattaca acttcacggt ggatatggat ttataaaaga ttatgaaatc
    10621 gaaagaatgt acagagatgc tagaatcgta tcaatatacg aaggaacatc agaagttcaa
    10681 aaaatggtaa tttcatcaaa cgtattaaaa taataaataa tctaatgaaa ttggaggtat
    10741 agactttgaa aatattagtt tgcgtaaaac aagttccaga tactaacgaa gtaagaataa
    10801 ataaagaaac aggtactctt ataagagatg gagttccaag tatattaaac ccagatgatg
    10861 caaatgcatt agaggaagct ttaaaaatta aagatgagca tgatgatgta actataacag
    10921 ttataacaat gggacctcct caagcagact ttatgctaag agagtgttta gctatgggtg
    10981 ctgatgatgc aatattatta agtgatagag catttggagg agctgatact tgggctacat
    11041 caaatactat tgcagcggga atatctaaag taggtgacta tgacataata tttgcaggaa
    11101 gacaagcaat agatggagat actgcacaag ttggaccaca aattgcagag aaattagaca
    11161 ttcctcaagt tacatatgta caagatttca aaatagaagg aaaagatata atagttcaaa
    11221 gacaattaga agatggatac gaattaataa aagtaagtac acctgtactt ttaacagcag
    11281 taaaagagtt aaatacacct agatacatgt ctgttgataa aatagtaaaa gcatataaac
    11341 atgatgtaaa agtttggaca attgatgatt tagatgtaaa taaagaagaa gttggtttaa
    11401 aagcatcacc aactaaagta tttagatcat ttactcctga accaagagga aaaggagaaa
    11461 tcttagaagg taaagctgat gaaatagcta gtaaaataat aatcggtcta aaacaaaagc
    11521 acattatata agttggagga gtacaaacta tgaatgatat aaaagattta agttcttata
    11581 aaaacgtatg gatatttgca gaacaaagag aaggaaaaat agctccagta gttatagaat
    11641 tattaggaga aggaagaaaa ttagctaaag aagtagatgc agaactttgt gcaatattat
    11701 taggaaaaga tgttgatgga ttagctaaag aattaatcac ttttggagct gacaaagttt
    11761 atgttgcaga tgatgctctt ttagaaaaat atacaactga tgcatataca aaagtaataa
    11821 aagatgcaat agatgaaata aaaccagaaa taatgctttt cggagcaact catataggta
    11881 gagacttagc acctagaata gcttcaagag ttggaactgg attaacagct gactgtacta
    11941 agttagaaat agacccagaa gataagaaaa taaaacaaac tcgtccagca tttggtggaa
    12001 acataatggc tacaatcatt tgtccaaacc atagacctca aatgtctaca gttagacctg
    12061 gtgttatgga taaagctgaa aaagacgaaa caagaactgg tgaagttata gcattagact
    12121 acaaaataac tcaagatgat ataagaacta ctgttttaga aacagttaaa actaagaaag
    12181 atttagtatc tcttacagat gcaaatgtta tagtatcagg tggtctagga ttaggtggac
    12241 cagaaggatt tgaaatgctt aagaaattag ctgacaaatt aggtggagta gttggttctt
    12301 ctcgtgcggc tgttgatgct ggatggatag accattctca ccaagtaggt caaacaggaa
    12361 ctactgttaa accaaaccta tatatagctt gtggtatatc aggagcaata caacatttag
    12421 caggtatgca atcatcagat ttcataattg ctataaacaa aaacccagca gctccaattt
    12481 tagaaatcgc tgactatgga gtagttggag acttacatga aatagttcca atgcttatag
    12541 aaaaattaga tagcgttgat gatttattag aagctataaa agcttaatag atacaaacta
    12601 tataaatatt actgattgaa taagtcttat tagttagtaa cataattatt aaccctttga
    12661 taagttattg ctagtgtgta tttgtcagtt attaaaaagt ctgtttgaat aaaattttat
    12721 aatacaagat aatctggata atccttatat gtatattgct tacaaattgt gagttatatt
    12781 ctgtaaggat tatctttgtt tttttaaata aatcttaaat ttaattaaat tgtattaaat
    12841 agacaaataa ttactaaata tgtgataaaa tacaattaaa agcaaaatga aatttttaac
    12901 aaactataat cattaaaatt atatcttagt tacgttttaa tgaaataaac tacttgaaaa
    12961 gacgaatatt ataaggagtt gattatatgt gctgatggta tttattgtaa gcaaacaata
    13021 aattttgtat gtagttcaat aaattactta atgaaaggtg cgaatattaa tggataataa
    13081 tttaataggg cttatagaac atgtagaaaa tccagccatt ttatgtaaag aatcaggcga
    13141 aataatatac tgtaatcatc taatagatag tatttttagt tttctagata taaaaaagcc
    13201 tagaaatata aatgaattag attcaaattt tgacaaaaca gaaatactga cagatagtaa
    13261 aaagaaaata gctttcagag agttaaggat gactgcacat atatacaata tgaaagataa
    13321 taataatgaa aataatatag tttatttatt tgagaagtct ctaatatctg ataaagtaat
    13381 tgaagatata atagagcata tagatgaagt tgttgttgtg tttaacaaag atggggtaat
    13441 tgaaaagatg aacactgtca gtgatgagat attacctttt aaaagaactg aggtacttgg
    13501 tagaaatata acagaccttg tgagacaagg attagttgaa gaaccaataa tattaaacat
    13561 gcttaaagtg aagaaaaaga tttatagaaa tatcgtttac ccagatggga agcttattgc
    13621 atatacggca gttccaagat gggattctaa gggaaagctt acaggaggag tactaacagg
    13681 gagagatatt tcaagggtta ttaagcttga atctcagata aaatatagtg atatatcaga
    13741 agacacagag tatataagtc aaagtaagat tatggacaat ataaaaaaag ttgttaagag
    13801 agctgcagcc tcagattctt ctatatttat aaatggagaa tcaggtgtag gaaaagaaat
    13861 aatagcaaga acaatatata agtatagttc aaggagagac aaacctttta tagctataaa
    13921 ctgtggagct ataccaaatg aattattaga gtcagagttt tttggatatg aagaaggttc
    13981 ttttactgga gctaagaaaa aaggtaaaaa gggacttttt gaagaggcga atggtggaac
    14041 tattttcttg gatgagatag gtgagttacc aatgcagatg caaaaaaaat tacttagggt
    14101 tatacaagaa aatactatta ctagaatagg tggtagtaag cctataaaaa tagatgttag
    14161 atatataagt gctactaata tttctcatga agaccttaga aataacctta aatttagaca
    14221 agatttgtac tatagattaa gtgttattcc agtaaaaata cctcctctta gagaaagaaa
    14281 agaggatata gtaccattag taaattattt cttaaaactt tacaatgaaa agtacaatag
    14341 agaagtagaa gtttcgccta aagttattga gttattagaa gaatattctt ggccaggaaa
    14401 cataagagaa ttaaaaaaca tcattgaaag atttgtagta ttatcagcta aaaatgtaat
    14461 tggagaagat gaatttaaca tgctgataaa tttagatatg attgataatg aaacagatga
    14521 tttatcacca atagtggtaa atggaattat gaatcttaat gatgcatata aaatagtgga
    14581 ccaaataatg ataagtaagg ctataaataa gtatggttca ataacaaagg ctgctgaagt
    14641 tataggtata tatccttcta ccatccatag aaagataaag agtggacata ttcacgttta
    14701 gctgtcagtt taaaaagaca atataattaa aatattataa gatacataat ttatacaact
    14761 taaaagagat ggcttaaaaa taaatttaat tactttaagt catctctttt cttttataaa
    14821 tatttaaaag ttatattagt ttatagagtt agcagaacct aaaacatcat ctatcttgct
    14881 ttcaactaca gcttgtatag catctctacc agcaccaatg tattttcttg ggtcaaattc
    14941 ttttggattt tcagctaaga attttctgat agcagcagtc atagctagtc ttaagtcagt
    15001 atccatgttt attttacaaa cagccataga agatgctttt cttaacatat ctacaggtac
    15061 acctttagca ccagctatat ttccaccaaa ttcattacat gtagcaacag cgttttggtc
    15121 aacagcagat gcaccatgaa gaactattgg gaaacctggt aatttgcttt ggatttcttc
    15181 tagtatatcg aatcttaatt tagcttctcc tttgaatttg aaagcaccat gagaagttcc
    15241 aatagctatt gctaatgaat caacacctgt tctttcaacg aactcaactg cctcagctgg
    15301 ttgagtatac ttgtgaacat cagaagtaac atcatcttct gttccagcta gaacaccaag
    15361 ttctgcttca acaacaacac ctttagagtg tgcatattca acagcttctt tagttattct
    15421 aacattttct tcaaagtcaa aatgagaacc atctatcata actgaagaga aaccagcatc
    15481 aatacaagtt ttaatagcat ccatatttgg accatggtct aagtgaagag ctacatctac
    15541 acctatttca tcagaagcag ccttaaccat ttcaactaat gtatgaggtc cagcatattt
    15601 aacagctgac atagaagctt gtatcataac ataagaattt ttagctttag cagcttttaa
    15661 aacaccttgt aattgttcta agtcacttat gttaaatgca cctatagcaa atccaccttc
    15721 gtaagctttt ttaaacattt cttttgtagt aattaatgcc attttaaact cctcctatta
    15781 acttttaaaa tttacccatc acttatatta taacgttaaa tcaaaaaaag tatatagtaa
    15841 attgtaataa ctataaatta caagtataat gttaaataga agacaatagg attaaataaa
    15901 ataataaaga atataaacaa tttaggaaaa ctagctatat aatgctaact ataagggtag
    15961 gaactacctt attagtttgg agatactgat aacaatagtt gccttgacca agaagttacc
    16021 acttcaaacg ttatcgaaag gaattaagtg gatgataatt cactaaaaat ttaaaataaa
    16081 ggagataaac tatgccacag ataaaaataa gaggaattaa tgaaaatgat atatgtaaaa
    16141 taagtgaaaa aatgataaat gatttagttg aagctgtaaa atgtccaaga gactactttg
    16201 aaatagaatg tataaagtca gttgcaataa gagatggtaa aatagcagat gtatatcctt
    16261 ttgttgaagt agcttggttt gatagaggac aagaagtaca agatatagta gcaagaataa
    16321 taactgatag tataagaaat aatttagatg tagaaagtat agatttagca tttacagtat
    16381 ttgaaaaaga aaaatattat gagaatggag agcattttta atggctaaaa agcaacgtat
    16441 agataaaatt ctttcaaatt taggttatgg aagtagaagt gaaataaaga agtattgtaa
    16501 acaagggtct gtagtagtaa atggaagtga agtttcaaat ccaggaactc aagtagacac
    16561 tgaaaatgat gagatattat ttaatggaga agaagttata tatagagaat atatatattt
    16621 gatgatgaat aaacctgatg gatatatatc agcaactaca gataaatatg acccaacagt
    16681 attagattta attgatttat catacttagc ttttgaacca tttcctgttg gaaggttaga
    16741 taaagacaca gagggattat tagttttaac taatgatgga aagctttctc atagagtttt
    16801 atctcctaag aagcatgtgc ctaagactta ttatgcaaaa attgatggag tagttactga
    16861 agaagatgta gaagcttttt tagaaggtgt agtgcttgat gacggatata aaactatgcc
    16921 atcacagtta aatatattaa aaagtgatga tgaatcagaa atagagctta caatacatga
    16981 aggtaagttt caccaagtaa aaagaatgtt tgaaagcgta ggcaaaaaag tagtatactt
    17041 aaaaagattg tctatgggca atttaaaatt ggatgaaagt ttagagttag gtgagtatag
    17101 agagttaaca gatgaagaag taaaattgat tgaagaaaga taataaagat actatagtaa
    17161 attggaggaa tgtctgtgaa gaaaaaagat gttatagaat ttgaagtaga taaaatggaa
    17221 tttggtggta catctttaag ccaagttgga gatagagtta tccatatgaa gggtggaata
    17281 agtggtcaaa aggtgagagc tggagttaaa aaagttagaa gtaagaaagc tgaagttaag
    17341 atgatagaat tattagagaa ctcaccactt gaaactgaag aaacttgcaa gcactttaga
    17401 gagtgtggag gatgtacact attgtctgtg ccatatgaaa aacaattaga aataaaagaa
    17461 aaacaagtta tggatttatt tttaaagcaa gatttatttg gatttcagtt tttagggata
    17521 gaaggaagtc cagaaaacaa atattataga aataagatgg aatatacttt tggagatgaa
    17581 gtaaaaaatg gacctttgac attaggtctt cataaaaaag gaaagcatat agatatacaa
    17641 actgttgagg agtgtatgtt ggttgatgaa gacttctcaa agatattagt ttctagtgta
    17701 gagtttttca atgaaaaaaa gcttccttat tatagaacaa tgaatcacaa aggatatttg
    17761 agacatcttg tagttagaaa agggattcat actaatgaga ttatggttaa tatagtaact
    17821 tcttctcaag aagattttga tatgtatgag tttaaagata tgcttttagg gattgattta
    17881 aaagctgagt tagtaggtgt acttcatact attaatgatg gtttagcaga tgcagtccaa
    17941 tgtgatgagt taagagtatt gtatggaaga gattatatac aagaagaaat tttaggtctt
    18001 aagtttaaga tttcaccatt ttcgtttttc caaaccaata ctaagggagc agaggttctt
    18061 tatagtattg ctagggattt tattggagat tacaatgata aagttgtatt tgacttgtat
    18121 agtggtactg ggtctattgg acaggttatg gctggtgctg ctaagaaagt gtatggtatt
    18181 gagatagttg aggaagctgt tgttgctgct aatgagaatg ctaagttgaa tggacttact
    18241 aattgtgagt ttattgctgg ggatgtggct aaggttgtta aggatttaaa ggacaagcct
    18301 gatttgatta ttgttgaccc tcctagacca ggcattcata aggatgctat tagggatatt
    18361 tgtggatttg gtgctaagga gattgtttat atttcttgta atcctaagtc tttggttgtt
    18421 gatttggttg attttaaggg gtatggttat gagattaaga tggttaagtg tatggatatg
    18481 ttcccaaata cgccacattg tgagactgta gtgttgctac gtcgaaaata atgctagaaa
    18541 tcttgagctt tagacagttt catagacata taatatttag attagagggt aataaaatta
    18601 ggaataaaga attgaaaagg tgttttgatg tgtctgtagt tatatatatg gagtgtggga
    18661 atacgaaata aaaaaagttt taattataga aatagatatt aaatttataa atttaatatc
    18721 tattttttat gtgaaaatat atttaatcta aagaatgata tttcataaaa aaaattaaat
    18781 atatttttaa aatatttaca atagagttat tttttattcg tataataata gaaatatttt
    18841 agcataagat gtataataaa ggaaaaaata cagaattaaa aaacataaga tagataagtg
    18901 gacttagggg agagaaatta ttatgcaatt gacaaaaata attcttagta actataggtg
    18961 ttttggagaa gaacctacaa ctattgaaat taataattta actggattta taggtcataa
    19021 tagcagtggt aaaactgctt tattaagtgc acttatgaaa ctttttggag aaaagtctag
    19081 tgatagaatt attgaaagat cagatttcca tatacctgaa agtattgatc cagatagtat
    19141 ttctaagaat gaattatata ttgaagcaca atttgagttt cctgaattgg agagaggtaa
    19201 agacagcata tctataccag tattttttaa aaatatggtt gttagtgaac caggtgggaa
    19261 accttattta agaattagac ttgaagcaaa ttgggagaaa agctcaaatc ctgaaggaag
    19321 tattgattca aatgtatact atataacatc tacaacagag gagataaatc ctgaagataa
    19381 gcgtaatatc aaaagatatg agcttgatca aataaagatg atttatgtac ctgccattag
    19441 gagaccagat gaacagttaa aaaatgtctc aggaacaata atttcaagat tattaaatgg
    19501 tataagttgg tcagaacaaa caaaggcaag aattaaaacg aaagcaagtg aaacggagga
    19561 aattttcttt gaagaaaaag gggttgaatc attaaagaaa tcactcgaaa ctcaatggaa
    19621 tagttatcat aaggatatta gatattctaa tccaaagata aacttcaata gtactgattt
    19681 agagacaatt ttaaaaaaag ttgaagtaac tttttcacct acagggacac atcgagatta
    19741 tcgggttgaa gagcttggag atgggttacg ttctttattt tatctttcac ttgtaaatac
    19801 atttttagaa attgaagaag aaattctaaa atctattaat gatggtaaag aagaagatat
    19861 atttacatta ataccgcctg tattaacaat tgttgcggtt gaagaaccag aaaatcatat
    19921 ttcacctcat cttttaggaa gggtagttga aaaattaaat tttatttcta caaaagaaaa
    19981 ttcacagaca atattgacat cacattcacc agcaatagta aaaagaattg aaccaacaga
    20041 cataaggtat tttaggattt gtaaggaata tgaatgtaca aaagtgaagt caattatatt
    20101 accaaatgaa gaaaaggaag cagaacaatt taaatttgta aaggaatcag tattagcata
    20161 tcctgaactt tattttgcaa aacttgttgt attgggcgaa ggtgatagtg aagagatcat
    20221 tattaagcag atgattgaat taaaagcaga atctgtagat tcatcagaaa tttcaatagt
    20281 accattaggt ggtaggcatg ttaatcattt ttggcgttta ttaaatgatt tagaaattcc
    20341 atttattact ttgttagatt tagatagaga acgagatgga ggtggttggg gacgtataaa
    20401 gtatgcaata caacaattaa taaatattgg tcatgataag aatgtattac tttatacatg
    20461 tgaaggaatt tcacaagata atgaatttaa agagatgcat acttgggatg taactgatac
    20521 tgaaaatatg gctaaatgga ttgaaatgct aaaaggttat gatgtttatt tttcagctcc
    20581 gttagatata gattttgcaa tgttacaaag ttttaaagat aaatatattt cagcattaac
    20641 aagtgcggaa ggtcctagga taaaagatca tggaaaaata caagatattg tgatcaatga
    20701 agatgctgaa gatgaatcat tgaaagctgc ttataaagat agaatagata gtgatattaa
    20761 atcaacctta aaacaagaag gtggtgatgg atctacttat actgagggag agaaagaact
    20821 aatgatatgg tattcatatt tctttttagg aagaggtaaa ccaactactc atttatcttt
    20881 attttctaat aatactgata tagattttga aagttttcca tactgtctaa aggagtttgt
    20941 aaatagtatt attgaaaaga tgatagatga ttcagggggt ggaagaaaat gaagaggaat
    21001 tgggttagta aagaagattg gagtccagcg gctggattca gactagaagt tgaagcgatg
    21061 aatacagtta ttaatgataa gaatacactt gttgtagctg gtcctggcgc tggaaaaaca
    21121 gagttattag ctcagagagc ctgcttttta ctagagacta atacctgtag atatcctaaa
    21181 aaaatccttg ctattagttt taaaaaggat gctgctgaaa atttaaaaga gcgtttagaa
    21241 ttgagatgtg ggaaagaatt agcttcaaga tttgaatcaa agacctatga tgcttttgca
    21301 aaagaaatcg tagatagatt tagaaatagt ttagatgaag catatagacc tttaaataat
    21361 tacaatatag ctgaaaatcg tgatgttaga agagcatttg agatagcagg attaaatttc
    21421 agaggaaatc aaactgaatt taataaagta tatggaaata aactatcgat aaataaattg
    21481 cctttacata cagattttgt agataagatt tttatagatg cttggaatgt actacttaaa
    21541 ggcaacaaag atactaattt tgtaagttca cttacttttg gtatgatatc acgtttagcc
    21601 gaatatttga taagaaccaa taggtatatt aagaaagcaa ttgaaatgac ttatagtcat
    21661 gtgtttttag atgagtttca agatactaaa tctattcaat atgatcttgt taaagcttgt
    21721 ttttttaaaa cagaaacaat aattactgct gtaggggatg gcaaacaacg aattatgtta
    21781 tgggctggtg ctagaaagtc aatatttgaa gattttcaaa atgatttttt aagtcaaaaa
    21841 agaacattaa ttatgaatca tcgttcagcg ccaagacttg tagagattca aaaagcaatg
    21901 tataacagtt tgaatgaaga ggcaattgat ttaaaaacta atgaaaagtg gaattatgaa
    21961 gatggagaag tgtatttaag actttttgag gattatataa aagaagctga tatacttagt
    22021 gaggaaatag tcgaattggt ggaaacaggt atagaaatta atgaaatatg tatattagta
    22081 aaacagagcg tagatattta ttcaaaagag attataaata aactttctga acgtaatatt
    22141 aaggcgcgta atgaagcaat gtatcaggag atattaaaag aagatataat aaagacactg
    22201 gtttcgcttt ttaggttgtc gttaagtgat agaaatcccg atgattggga ttatatttac
    22261 gatattctag gtgaattata tggaatagac gaagggtata accctaaaat attaaatgat
    22321 ttcattacca aagtagaatt aatgattaat gatctcaagg taaatttaaa tattactgat
    22381 gaaaaacaat tttcagattt agttgattct aaggttgaat atatttcata tgaaaaattt
    22441 gcatcaaagt ttcaaaaata taaatctaaa gattattttg atgatttggt agataaattt
    22501 aaaaagctgg tatggattga atataatgaa actaaagatt ggattaaagc gattgataac
    22561 tttgaaggta aaaattcaat tccaataatg acaattcata aaagtaaagg acttgaatat
    22621 gatactatct tttttgttgg gttagaagat tcagcatttt ggaattttaa aaatcaacca
    22681 gatgaagata gatgtgcatt ttttgttgca ttatcaagag ctaaaagaag aattgatttt
    22741 acatttagct tagatagacc agcaggattt tcgaattcac agaagcatta ttgtattaat
    22801 gaattctacg aattgtttga taatactgag attgtaaaaa agatagagta ttgattaaga
    22861 tgataaaaac tttagtatat tatattttaa gatattagtt atataagtag acattgaatt
    22921 tatgtatttg atgtctattt tttatataaa aatatattaa agaaaggatg aatttgcatg
    22981 aagaaaaaaa caaaaatatc taaaaagatt gtaagtttta ttatggtttt tataactata
    23041 gttactacta tacttacatc atcagtttca gcttatgcta gcgagttgga tatgagtgaa
    23101 cagacaggct ttaattattt aggtattagt ccaattacag gaaaaaaatt aatcataaca
    23161 tttatcgtat gaagatggat aataaaattg tattttgtgt agagccagga attaaaactt
    23221 caagtggagg aggatatata tcggaggagt taggagataa atttatatct acaacacttc
    23281 caaattatca taaaagaaaa gctgaaatac ttgagaaggt aaatagacat aatatattac
    23341 cttcatggaa taatgaaaaa gttgcagtta aaagtggaga aacaattgag ttagaagata
    23401 agaatggtat tattagagaa atgcaattag aatcaaataa tactggaaca gatatattac
    23461 ttgaagaaaa tactttgaaa attacagcaa ataaaaattc taattcagga gttattagtt
    23521 atataaagat tcctaaaaat gaggttggaa tatcaatagt ttatcgtaag cataactatc
    23581 aaagcttatc agaatttcat atggaaagtt cgatgagtgc aaatttaaat ataaatgtaa
    23641 tcaagttagg aattgttaaa gtaaaaaaga ttgatgaagg cacaggaaag gaattagcaa
    23701 atgcaaaact tagatttgag tatgatggtg atagtaaaga aattataacg ggttctaatg
    23761 aaattgctag tatagaagat attattggtt gattaaaaag tgaagtatac ttttgtatct
    23821 atgaaaaaga ctataagcaa tatataaaat tagatatacc tattgatgaa gcaaaaataa
    23881 aaaatagatt tgaaataagg ttaaaaaatg aaagagcata tcatgcagca gttgatttgt
    23941 tagcaaatag agatgaagaa aagacagcat tttcaattat taatcgttat atacgttttg
    24001 ttgataaaga tgatgaaaaa attagaagtg aatggaaaac taatgatcag tggagttatt
    24061 ttattggtga aggtagagcg aagttaaaac taacaacaaa tccagaacca tataatattc
    24121 aaaaaacttt aaattggtta tcaagacaag ttgcaccaac gttaaaaaca attaaaaggt
    24181 tagatagttt gaacaactca acattaattg agggcttaat aaggtatgca agactaacag
    24241 ataaacataa aaaaataatt gaacaaaatt cagtaaatgt tgaagacatt atttatgatg
    24301 aataaaaaaa tgtaattagt attgcagatt ttaatataaa agaagggaga tatacgaatg
    24361 aattttggtc aaaatttata taattagttt ttatcaaatg cacaatcatt ggtattaatg
    24421 gctatagcta taattggtgt atatttagga tttaagagag agttttcaaa acttatagga
    24481 tttttagtag ttgcattaat tgcagtaggg cttgtgttta atacagcagg agttaaagat
    24541 atattattgg agctatttaa taaaataata ggtgtgtaag gattaaaaga gaatagaaaa
    24601 ttatgagtat atgatgaagg gttctaataa gttttattaa aatatatatt gaaagacaag
    24661 tagagctatt tcgagttatt acatgtaatg ctaatatata taactgcatt ttggaaatta
    24721 aatacataat tagaaatagc aaattttata ggtgacttat taatatgttt acttattgta
    24781 tactatttga tatatttaat atgaggtgat tcttgaaaat ggacatagtg aaagtttttg
    24841 ctaacaatgt gagaaaatat agaaataaaa aaggtctatc acaagaagct tttgctgaaa
    24901 aagctggatt gcatagaact tatatttgtg ctattgaaag aaaaaagaga agtattgctt
    24961 tagataatgt gcagaaaatt gctgatgcgc ttgagattga gacttactta ctatttatag
    25021 aaggtgagga gaaagaatgt ctatgaaaaa attaccaata gaaaagctta tagaaaattt
    25081 aggtttttca gggtcaaaga atcttgtgaa tatagatatg cttacaaaat tagatatatc
    25141 taaacatgat gtaaaaactt taaaagttat aaatccttat gcaatttatt tagtaaatag
    25201 taagccattt atattattta tagagtcttc ttttgaagaa aaaaaattac aacaaattag
    25261 taagcttata tggaatgctc aaataccaat tgcttttata tgcgattcta attctgttaa
    25321 aatatttaat ggaaaagctt tggatttaaa gtcatattta attgaggaaa tatataatca
    25381 atctattgag gatttttcgg aagaatctga gttttcttat ttgaaaatat ctgatccatt
    25441 attttgggat caatacacta aacaatatac caaaacgcat ttaaatgatt atttgctaga
    25501 taatattact tcattgacaa ataaattaaa gaaaaaatat aaggtgaaat ttgcaacgaa
    25561 gcttgttcta agattaatat ttattagata tttaatcgat agaggtgtcg atatagctta
    25621 taaaaatttt aatcatgata ttaaagcttc acaaaaagaa ctgctaaaga ttactaaaaa
    25681 caaagttgaa ttatatgaac tgtttaaata cttaaaagaa aagtttaatg gtaatttatt
    25741 tgagttaaat gacgaaatta ataatacttg tttaacagat gaagtgttta ttttactatc
    25801 tgatttttta tcaggtaaaa taagtattga caatggacaa atgtctttat tttcaatgta
    25861 tgactttaat atcataccag ttgaactaat tagtaatata tacgaaattc ttttaggaaa
    25921 agaaattcgt aataaagata acgcattcta tactccgaat tacttagcag aatatatttt
    25981 agataaaaca acacttgaat ttctgaaaaa taataggcaa tacaaaattc tagatccttc
    26041 ttgtggatca ggagtatttt tagtaaatag ttttagacgt attattgata tgaatttggg
    26101 tgataaactt ttttgtgaag atgataaact tttaaaagaa cttttattat ctaatattta
    26161 tggaattgat atcaatgaag atgctatcga tgttactatt ttctcgttat atctaactat
    26221 tttagattac aaagacccta agatattatc agaatttaaa ctgcctaatt taaaagggtg
    26281 taatttaata gcgagtgatt tttttgatga taataaattg gagagtttaa agaaaattaa
    26341 ctttgatttt atcattggaa atccgccttg gggaagtgtc tcagatggat tacatttaga
    26401 ttattgcaat aaaaatggct ataatgatat gcaacaaaac aaagaaatca gtagaagttt
    26461 tatttttaga gcaaaagatt ttagctctag aaatacagta tgttcttttg tagtgcattc
    26521 taaaatacta tatactcaaa aaaagccatc aaaaaaattc cgtgaatttt tgcttaaaaa
    26581 aagtcaaata tttagtgtag ttgaaatgtc atctgtaaga aaattagtgt ttaaaaatgc
    26641 agacgctcct gcagcagtta tatcatttaa gtataacgaa agtgaagaaa atttaaataa
    26701 tagaatcatc tatacatctt taaaaccaaa tgtatttttt aaattattta acattattgt
    26761 aattgaaaaa tatgatgtta aatatatttc acaaaaacta ttattggaga atgattgggc
    26821 ttggaaaact attgtgtatg gattcagtaa agatatagat ataattatga atatcaaaaa
    26881 aaaatatcaa actgtaaatg aaattatttt gaaaaacagt tttgttcatg gaacagggat
    26941 acaaatcaat ggtaatgaaa gccatgatgc gacacattta aaaggaaagt ggtatgttga
    27001 ttcaaagaaa ggaatagatt cattctatgt tgatataaat aaagggaaag aacttactca
    27061 agataaaatg agaagaccta aggctaatca gcaagaaata tttattcctc catatgtatt
    27121 attaaaaaag ggagttgaca cgaaaacata tagatataga gcagcatttt caaatgatgc
    27181 atttgtatat tcagatgcag ttacaggaat atgtggaaaa aaagataatt taactacact
    27241 ttattgcttg actggtatgc ttaactcttc tttttactca tacttaaata taatgctagg
    27301 tacatcaatt ggaatagaaa gagaacaagg attttcaacg gaaattttta cgtatccagc
    27361 aatagaaagt gatgaaattg ctaatctggt agtaaaaata caagaagcga ctaaattaga
    27421 aaaaacaact atgttcttga atgaaaaaaa tagttgtaag tatatcaaac aattagataa
    27481 gttaattttg aagtctttta atttaaatga tgataaattt attgactatg caataaatat
    27541 tcaaattcct gagcttacaa attctaagtc aactaacata tatagaaaaa taactaagga
    27601 agatttaatt aattatagtg aatgtttcaa agaacagttt tcatcgatat atcgtagggt
    27661 tgggaaaagt atttcaattt cggtatatcc aaaaatatta aatcgtttta cagtatttga
    27721 actagaagta acagatgaaa aaacagatag agagattatg gttaaagcag aagctgataa
    27781 taatatggag ttgtttacga aaataattag atatgattat aatgatgttt ttcatcaaat
    27841 tagagatatt attcattttg gagaaaattc attttatatc ataaagccta attgctataa
    27901 atattggcat cctgctatcg cagaaatgga tcttgctgat gttttggatc agattttatc
    27961 tgatacaggg ggtgaagaat aatgggagga tttaaaaatt cttcactaaa cttttattta
    28021 aagcaagggc tttatgaaat aattatagat ggattaatag actgttgttt acttatgagc
    28081 aaatcatgta ttgaacaatc tagaaaaata ttaaatcacg aagaaaagat aagaacgcat
    28141 ttagttgaaa attatttaga aaatgatatt gaaagaagaa aaacatcttt atctaaacaa
    28201 catctaagat ttgaagtaga agcacaagaa aattatgaca acaaaaatga atcttatgag
    28261 ggtagggttg atataaaagt tatatctaaa gacactttag cggatagaag ggcttattat
    28321 atcattgaat gtaaaaggat agatggtaag gaaactttaa ataaaaaata tataaccgaa
    28381 ggtgtacaac gatttattac tgagaaatat tcttcgcatt atggcaaaaa tataatgctt
    28441 ggatttatag taaaagatat aaatatccag agcaatatac aaaaaattaa ttcactgaat
    28501 cagaacatat tatatacata tatagataca gatatgttga cagttagaag tgattctgaa
    28561 aataattatg ctgtttgtga aagtaaatat aaagtaaaat ctaaactact attgttaaat
    28621 catatttttt ataacttttc gagtataata agataatctt atgaatataa aatattacta
    28681 tgttaaaatt atagtttggt tttgataata ttataaaaat aagtttaaga ataaaatata
    28741 aaggaaaaaa ggggcgtagc actatttttt agtgtgacag cccctttaat tttaaaatcg
    28801 gtaaggagga gtgtatttga aaaaaattaa gtcatataca gggattttga atgtggagaa
    28861 ggttatatat gcaataaatg attttcagct tccatttcca gttactttta cgcagatgac
    28921 atggtttgta ttaacacttt ttataattat attatttggt aagttacctc cattatttat
    28981 gatagaagga gcatttttaa agtatcttgg cataccaatt tttataactt agtttatgtc
    29041 tcaaaagact tttgatggaa agaaaccata tggattttta aagtcaataa tactatattc
    29101 attaagacct aaaataacat acgcaggaaa aaacattaaa tttaaaaata aaattattga
    29161 tgaaaatata actatagtaa ggagtgaatt atatgttccc gataaaatat atagagaaca
    29221 atcttgtatt taataaggaa ggagaatgtt ttgcatatta tgaattaata ccatataatt
    29281 attcattctt ataagctgaa caaaaattta ttgtacatga taattttagg caattaatat
    29341 cacaaagtag aaatggtaaa attcatgcac ttcaaatagc cactgaaagt tctataagag
    29401 ttacacaaga aaggttaaag aaaaatatta caggaaaatt aaaagaaatt gcttatgaaa
    29461 aaatagacga gcaaacagag gcacttgttt ctatgattgg cgataatcaa attgattatc
    29521 gtttttttat tgggtttaag cttttagtta cagagaaaga aatgaatata aagagtataa
    29581 aaaagtcaat attagctaat ctatcagatt ttatatatga tgttaataat aaattgatgg
    29641 atgactttgt cagtataaat aatgatgaaa ttatgagatt tttaaaaatg gaaaagttat
    29701 tagaaaataa aatctcaaga agatttaaag ttagaaggtt agataaaaat gattttggat
    29761 atttaattga acataattat ggaaagaaag gcacagtttt tgaagattat caataccatt
    29821 tacctacaaa gaaacttaaa agagaaacat tagtaaagaa atacaatctt ataaagccaa
    29881 ctagatgtct tatagaagag aagcaaagat acttaaaaat tatacatgag gattcagaaa
    29941 gttttgtagc ttattttact attgaaatta ttattggaga aatgaatttt ccatctagtg
    30001 agatattcta ttatcagcaa caacagtttg catttcctat tgatactagt atgaatgtgg
    30061 aaattgtaac aaataaaaag gctttaacta cagttagaaa taagaaaaaa gaattaaaag
    30121 atttagacaa tcatgcttgg cagtcaggga atgaaacagt aacaaatgta gtagaagcat
    30181 tagaggctgt aaatgagctt gtaaataatt taaatcaaac aaaagaatca atgtataagt
    30241 taagctatgt aattagggta tcagcagata gcattgatga attaaaacgc cgttgtgatg
    30301 aagttaaaga tttttatgac gatttaaata taaaattagt acgcctaatg ggagatatga
    30361 taggatttca caatgaattt ataccttcaa gcaaaaggta tataaatgat tatattcaat
    30421 atgtaactag tgattttcta gcaggattag ggtttggagc aactcaatag cttggagaga
    30481 acgaaggaat atatattgga tatagtttag atacaggaag aaatgtctat cttcaaccat
    30541 ctcttgcaag tcaaggagta aaagaatctg taactaatgc gttggtagca gcattctttg
    30601 gatttttagg aggtgggaaa tcatttgcta ataacttact tgtttactat tcagtattgt
    30661 ttggagggca agctgttttg gtggatccaa aagctgaacg tggcaaatgg aaagaaaatt
    30721 taccagatat atatcatgaa gtaaatattg ttaatttaac aagtgatata gaaaataagg
    30781 gaatgctaga tccttatgta attatgaaag aagtaaaaga tagtggaagt ctagctattg
    30841 atatattaac ttttcttaca ggaataagta gtcatgatgg agagcagttt cctatactta
    30901 gaaaatcaat cataaatgtt actcaagaca aatgcagagg acttttgaag gtaatagatg
    30961 aattaaggaa agataattct caaatatcaa ataatattgc agatcatatt gaaagtttta
    31021 cagattatga ttttgcacaa ttacttttct cagatggaaa tattaaaggc tcaattaatc
    31081 tagaaaaaca aatgaatata atacaagttg cagatcttgt tttaccgaat aaagatactt
    31141 tatttgaaga atatacaaca atggaaagtc tttcagtagc tatgttgata gtaattagta
    31201 cttttgcatt agattttatc catacagata gaagtacttt taaaatagta gatttagatg
    31261 aagcttggac atttcttaat gtagcacaag gaaaaacttt atctaataaa ttagttagag
    31321 caggatgtgc tatgaatgca ggagtgtatt ttgtaactca aaatgcaggt gatgtatcag
    31381 atgaaaattt aaaaaacaat attggattaa aatttgcatt cagaagcaca gatatcaatg
    31441 aaattaagag aatacttgaa ttttttggag ttgataagga agatgagaat aatcaaaaaa
    31501 ggttaagaga gttagagaat ggacagtgtt taatacaaga tttatatgga cgagttgggg
    31561 taatacaaat acatctagtt tttcaagaac tccttactgc atttgataca agaccaccta
    31621 taagaaaaga ggtggattaa tgaaatcgaa agtatttaga aatttaagct ggataactat
    31681 tttaatagta gtacttttac tattttttat ttatataaat ttatagattt agagaaacaa
    31741 atagatacat tagataagaa aagaaaaaaa ctcatagata tgagattaga agatataatt
    31801 gataaagaat catatgaaga aaagtatgat gatttaacag ttgaaataaa gaaacttagt
    31861 aaagagaaaa aaactcttaa agcatcatta ataaatgaaa agagtataaa agcaagactt
    31921 agagaattta gaaagatact tgaaagaaat gaattgttag aaaaatttga tagatatgta
    31981 tttgcaagta tagtagataa ggttatagtt ggagttatta atgaagatgg tatagttgat
    32041 ccttataaaa taacttttgt ttataaaact agctttgatg ataaacaaga tggacataaa
    32101 tttataaata agagtccgtt agaaacgtat tcaaaagata acaacgataa taaaaagaag
    32161 tgttcatata ctaataatga gacattgcga gactgtagtt aaacttatta gagaataaca
    32221 aggtttcaga cttttatatt gtgtcttaaa agtagatttg ccacctattt gccaccataa
    32281 ttatttttgg gtggcaaatt tattctacaa tgatttcaaa aatatttacc gtctggtttc
    32341 tcatgttgtt agttacatgt aaatatgtat ccatagttgt tgaagtttta gcatgtccga
    32401 gtatatgctg gatatctttt atattagtac cattttctaa taacatagta tcagggtttt
    32461 taaaataaag tgtgtattct tctaaagtag tgataattaa aatatcaaat atcactatac
    32521 aaaggaaagt gcacgctttt tatgtttaac aaaaacgaaa tattaaaaaa cttattgaag
    32581 aatatgatgt caaaacaact acagatattc aagatatgct aaaagattta tttacaaata
    32641 caattcatca aatatttaca ttgtcgaatt ttagcggttt aaacataata tattgactga
    32701 tataataaaa tcatagaata ttttaggggg aaatagatta tgtttaagaa aaagttaata
    32761 gctgttgcaa tgagtataac tatgattagt gtagggagct tttcgtatgc tcattcagga
    32821 agaactgatt catcaggagg tcatcgtgat aataaaaata aatctggatt aggaagttat
    32881 cactatcatt gtggtggtta tccagctcat ttacataata atggtggttg tccttatact
    32941 ggaggaggaa gcagttctgg aacaacaact tcggttaata atgaagaaaa gcaaaagagg
    33001 tctgttggag aaaaaggata taatcaaggg tatgaagatg ggtataaagg aaattatagt
    33061 tcatcaaatt atagtggaga ttatagtgat acatatgaat caaagtactc agaaggatat
    33121 gaaaaaggta aggctaaatt agaagaggaa gaaaaggtgg ctaaagagac tggatataat
    33181 ttagggctta caggtgctaa atctaataat acatatgaaa aagaagtttt aaaaaatgct
    33241 tatgataccg gatattcaac tggatacaat gaatataaga ctaaaaagat agaagaatat
    33301 aaagctaaag ggatagagga tagcaataaa gataaggaaa aaatgacatt tgaagaaaac
    33361 atagattctg aatttataga tgcttataat aatgcttatg acgaaataca agaacaatta
    33421 aaaaatgatt acactactca aggatttgag agtgctatca aaggagaaag atttgacaca
    33481 tcaactatag gtaatgttaa atatgctaat tggtttaaag aaggatatga taatggtaag
    33541 gttaagttac ctaaagcaaa agaaagtgta tataatcaag gttacaatga agaagatttt
    33601 agtataccag atgaaatgaa gtctattgaa actaagttaa aaggtgtata tgatgaaggt
    33661 ttagaaaaaa gagaagaaga gaaaagtaga aatgctactt atggggttgg agctggaaca
    33721 gcagctgtag ttgctggagt agtatataag aagaaaaaac gtaaaaagat atagatgcta
    33781 gatataccaa tgatgacaag tgtataacaa atataatttt aagtataaat atttaaaata
    33841 tagaagggaa ggttatatct atgggattta actttaggaa aagcataaat ctaggaggag
    33901 gactcaaatt aaatttaggt aagaagagtg taggtataag tgctggtgtt aaaggtgcta
    33961 gagtgtcagt taattcaaaa ggaagaaaat cagctacatt gagtatacct ggaactggaa
    34021 tatcatatac aaaaactagt acaaagaaac gtaaacctaa ataaatattt gatatactaa
    34081 gaaatagact gttacaaata gtctattttt tagtatatag atttaaaaag tgacaaaatt
    34141 ttgaaatata aactaatttc ataaaggaaa atattatttt atgtagaatt ataatattta
    34201 agtaatatta taaaatttat aggaggattt cattatgaaa ttaagcaaaa tactattggc
    34261 aatagggatg tctgtttcat tattgattgc tagttgtcca acatcaaacg ctcttagttc
    34321 tattaattca ataaaaggta caaataagta tgaaacagca ggtttaatag cagataaaca
    34381 aaattatgat attgcaatac ttattaattc tgaaaatgga ttatctgatg gattgtcagc
    34441 aagtggattg tcaggagcta aaaatgcccc aatattgtta actaagaaag atacaatccc
    34501 ttcagaaaca ttaaaaagat tagaaaaggt aaaagaagta tatctaattg gaggaaaaaa
    34561 ttcgatagga gcatcagttc agaaaatatt aagtgataaa aaaataagca taaatagaat
    34621 aagtggaact gatagaatag atacaagtca taaagttgca aaagaaatag gaagagtaag
    34681 tacggttaaa aaagtaatat taactaatgc ttttaaaggc gaaccagatg ctatgagtgt
    34741 tgcttctata gcagttagag ataaatctcc aatagtatta actgatggaa atactacaag
    34801 ttttgctaca gatggattag aaacttatgc aattggtgga aaagtcagta tgagtgataa
    34861 gttaataaat gaaactaaag caacaagaat aggtggagta gatagatacg atacaaataa
    34921 acaaataata aataaatttt ataatggctc aaaagaattt tacatagcaa gtgggactga
    34981 tttagtttat tctttagttg gttctacaat tgcacaaaaa acacctatag tgttagttgg
    35041 aaatggaagt aataagagta tattaaaaaa tgcaagtaaa ataactgcta ttggaaatat
    35101 tgatgatgtt gttataaaac aatgtttaaa tataactaat aatgttgggg attcaaatac
    35161 aggtattgta aaagatacta attcaaataa tacgaacaaa ccaagtaatg agcctagctc
    35221 taataatcct ggattaactg ataaaagtac agtatatgta actcctaatg gaaaatcata
    35281 tcatctaaca aaaaattgtt ctacactaaa aagaagtaaa acaataaata aagtaacatt
    35341 aaaacaagct aagtcacaag gtaaaataga tccttgtaat gtttgtgtaa aataatgtat
    35401 tagccaaata tatattaaaa aagacaatta attaagattg tcttttttat tgaagaaata
    35461 taaatttata ttatgtatta ttaaaaaaaa attattaatt ataaaaaata ataataaaga
    35521 cgataagtat taaaatttta tgtttccaat atttatgata taattaatat ggcaaggaat
    35581 aaatatttga aaaagctatg agtggtgttt ccatggaagt ttcttcactt tcttatgaga
    35641 ggaggtgaaa actatggaaa atctaatgat gagtattata actggtgtta tagccagtta
    35701 catttacgat aaattaaaga gccactctga gcgaccaact aagagtggct gggaacttaa
    35761 tataaagttc cagaaaagta aatcttcaaa ataatttgtt gaaggaaaca tcactcaaag
    35821 ttaagtaaaa tatgtttcct tgcttttatt atatcacaaa ttaaaaatat taacacaaaa
    35881 ttttgtgaat atatacttat tattgttcta aaaaaatatg taaataattg atttgattgc
    35941 atggaattat tgatgtaata tttaagttaa atatacacaa tctcaattaa agaagataag
    36001 tactataaat tcatatcaaa aaaatacata aacttttaag ttataatttt ataaaaagtc
    36061 ttttagtatc attatttcaa gaggaattat catatgaaaa aataacttca ttagaaaagt
    36121 ggataaaaaa ctggtaagaa gatgactaaa gattacatat actattactt aaatta
//
LOCUS       DAYATN010000023        35947 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.023, whole genome shotgun sequence.
ACCESSION   DAYATN010000023 DAYATN010000000
VERSION     DAYATN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.023"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            197..337
                     /locus_tag="ACGI8A_001158"
     CDS             197..337
                     /locus_tag="ACGI8A_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179161.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(512..832)
                     /locus_tag="ACGI8A_001159"
     CDS             complement(512..832)
                     /locus_tag="ACGI8A_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFH0179162.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(952..1689)
                     /locus_tag="ACGI8A_001160"
     CDS             complement(952..1689)
                     /locus_tag="ACGI8A_001160"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFH0179163.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1904..3727
                     /gene="acd"
                     /locus_tag="ACGI8A_001161"
     CDS             1904..3727
                     /gene="acd"
                     /locus_tag="ACGI8A_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HFH0179164.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISEGNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3942..8240
                     /locus_tag="ACGI8A_001162"
     CDS             3942..8240
                     /locus_tag="ACGI8A_001162"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFH0179165.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8444..8905
                     /gene="rimP"
                     /locus_tag="ACGI8A_001163"
     CDS             8444..8905
                     /gene="rimP"
                     /locus_tag="ACGI8A_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFH0179166.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8935..10089
                     /gene="nusA"
                     /locus_tag="ACGI8A_001164"
     CDS             8935..10089
                     /gene="nusA"
                     /locus_tag="ACGI8A_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFH0179167.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10096..10377
                     /locus_tag="ACGI8A_001165"
     CDS             10096..10377
                     /locus_tag="ACGI8A_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFH0179168.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10367..10678
                     /locus_tag="ACGI8A_001166"
     CDS             10367..10678
                     /locus_tag="ACGI8A_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HFH0179169.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10700..12640
                     /gene="infB"
                     /locus_tag="ACGI8A_001167"
     CDS             10700..12640
                     /gene="infB"
                     /locus_tag="ACGI8A_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFH0179170.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12683..13063
                     /gene="rbfA"
                     /locus_tag="ACGI8A_001168"
     CDS             12683..13063
                     /gene="rbfA"
                     /locus_tag="ACGI8A_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFH0179171.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            13044..14027
                     /locus_tag="ACGI8A_001169"
     CDS             13044..14027
                     /locus_tag="ACGI8A_001169"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFH0179172.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            14040..14945
                     /gene="truB"
                     /locus_tag="ACGI8A_001170"
     CDS             14040..14945
                     /gene="truB"
                     /locus_tag="ACGI8A_001170"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFH0179173.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15076..16005
                     /locus_tag="ACGI8A_001171"
     CDS             15076..16005
                     /locus_tag="ACGI8A_001171"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFH0179174.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16109..16366
                     /gene="rpsO"
                     /locus_tag="ACGI8A_001172"
     CDS             16109..16366
                     /gene="rpsO"
                     /locus_tag="ACGI8A_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFH0179175.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16606..17451
                     /locus_tag="ACGI8A_001173"
     CDS             16606..17451
                     /locus_tag="ACGI8A_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFH0179176.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17576..19687
                     /gene="pnp"
                     /locus_tag="ACGI8A_001174"
     CDS             17576..19687
                     /gene="pnp"
                     /locus_tag="ACGI8A_001174"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFH0179177.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19953..20696
                     /locus_tag="ACGI8A_001175"
     CDS             19953..20696
                     /locus_tag="ACGI8A_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0179178.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKAGKLSDVFK"
     gene            20854..22101
                     /locus_tag="ACGI8A_001176"
     CDS             20854..22101
                     /locus_tag="ACGI8A_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFH0179179.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINSVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22136..22381
                     /locus_tag="ACGI8A_001177"
     CDS             22136..22381
                     /locus_tag="ACGI8A_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HFH0179180.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22391..23614
                     /gene="dapG"
                     /locus_tag="ACGI8A_001178"
     CDS             22391..23614
                     /gene="dapG"
                     /locus_tag="ACGI8A_001178"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFH0179181.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23727..24461
                     /locus_tag="ACGI8A_001179"
     CDS             23727..24461
                     /locus_tag="ACGI8A_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HFH0179182.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24584..26995
                     /locus_tag="ACGI8A_001180"
     CDS             24584..26995
                     /locus_tag="ACGI8A_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFH0179183.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26998..28056
                     /gene="mnmH"
                     /locus_tag="ACGI8A_001181"
     CDS             26998..28056
                     /gene="mnmH"
                     /locus_tag="ACGI8A_001181"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFH0179184.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            28050..29384
                     /gene="rimO"
                     /locus_tag="ACGI8A_001182"
     CDS             28050..29384
                     /gene="rimO"
                     /locus_tag="ACGI8A_001182"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFH0179185.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29371..29913
                     /gene="pgsA"
                     /locus_tag="ACGI8A_001183"
     CDS             29371..29913
                     /gene="pgsA"
                     /locus_tag="ACGI8A_001183"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFH0179186.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            30201..31247
                     /gene="recA"
                     /locus_tag="ACGI8A_001184"
     CDS             30201..31247
                     /gene="recA"
                     /locus_tag="ACGI8A_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFH0179187.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            31423..32964
                     /gene="rny"
                     /locus_tag="ACGI8A_001185"
     CDS             31423..32964
                     /gene="rny"
                     /locus_tag="ACGI8A_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFH0179188.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            33278..35614
                     /locus_tag="ACGI8A_001186"
     CDS             33278..35614
                     /locus_tag="ACGI8A_001186"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0179189.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
BASE COUNT        14487 a         3816 c         6762 g        10882 t
ORIGIN      
        1 taaaaaagat aataatagtt agatttttta tatgaatttt aataggtaaa atatcctatt
       61 gttttgtaga tatatcctaa ttatgtcaaa aaataaaaaa atttttattt attagagttt
      121 ttggaaataa taataacaga tattataatt aattaacagt ttttgtaagt atttgacaat
      181 agagagggtg taacaaatgg gaataataga gtcagcatca aaattggctg agatggttca
      241 tttattggct gttgaaaagg ggataactga tatagaagca tgggatgaag ctgtgaaaga
      301 atacagcaaa atttatgaag aaagaagaaa tgaatagaat gataaaaaat gaaaacttga
      361 tgataaaaaa taactttatt taaagaaatt caatttttat agaagtatta aaaataatat
      421 aaataaaaag atggtatttc aaatgtaagt aaaaaatcat tttgggatac catccttatt
      481 atttatttta ataatatata agtaataaat cttacttatc catatttttt atgtaacctc
      541 ttttttctaa cttttttata tgctccttac gtatttcatc aagattaata ccatattgtt
      601 cttctagctt aaacatcata gtaacacatg tctgagctac atccattagt tccttagcag
      661 ttttctttat aatatcaact tcatctaaat cattattttc tccacttaga tttctgaatt
      721 tacctataca ttcagctaat tcaccttgtt cttcacccat tttgataaat gtagattcta
      781 gtgttgggtc taaattatta agctctggta aagatatagt ttttagtttc ataaaagctc
      841 ctttcaagtt attcgtattt atactaaaac atcataacaa aaataatact aaaaaaaaac
      901 tttaaattca acataaaaac ttacatatat aataaaaatc gtgaaataaa tctatatttg
      961 tcttaataca gcctcatata aaacttctcc aataggttta tttattacaa gagaagctga
     1021 cttatcatag actgtatttg ctttattaat aagagcaata taatcacctt tgtaataatc
     1081 tataaaactt gctgctgggt acacagctaa tgatgtacct cctataatca ataaatcagc
     1141 attacttata gcagaaattg ttttagtaat gacatcactg tcaagagctt cctcataaag
     1201 tactacatct ggttttacta tagaaccaca gttgtcacag taaggaatat tacctcccag
     1261 atttaacata gattctaagt caaaaaactt accacattta gtacaataat ttctatgtac
     1321 agaaccatga agttctagca catttttact tccagccatt tgatgaagtc catcaatatt
     1381 ttgagttatg acagctttaa gcttacccat ttcttcgagt tttgctaaag ctatatgagc
     1441 attattgggt tttgcattag gatatattaa tttatcctta taaaattcaa aaaattcttc
     1501 tggatatcta acaaaaaaag tatgtgaaac taattgttca gcagtaaatt gtttattgag
     1561 tttttggcta aataatccag tagaacttct aaagtctgga atattagatt cagttgaaac
     1621 acctgctcca ccaaaaaata caatattatt gtgattagct attaagtctt ttaagctatt
     1681 tgcatccata gttacacctc caaattatta tatataaatt gtactacata ttaggtcgtt
     1741 tatgttttat taatttaaat aaatatatag tttaataagt taaataaagg gaatataaat
     1801 aaaatgtata cagagtataa atagacaata tttactaaaa taacaagttg tcgtataatt
     1861 atattaaaat tgttttagtg tataagaagg gcgggggaag tacgtgaaga aaaaagcagc
     1921 tttagcaaca ttggctatgt tacccttagg tgtggtaaat gcacatgctg atggagatat
     1981 aggtatagtg actataaatt atttaaatgt aagaaatgaa ccaactgccg aaagtagcat
     2041 agcctttgtt gctaaaaaag atgataaagt tttgattaaa gactcttcta atggatggta
     2101 taaaataaaa gctgaatctg gacaagaagg ttgggcttca tcaaaatata tagcaaaatc
     2161 aaatagtgac tctttaagaa cgtctacaaa taaagaaaaa caagttattt caaatagttt
     2221 aaatatgaga aatggagcag gtacaagtta cagagtaata actgtactaa aaaaaggtca
     2281 aaaagtagaa gtaatatcag agagtaatgg atggtctaaa atcaaatatg atggaagact
     2341 aggatatgta tctagctctt atttaggaga cgtttcaaac tcaactaata aatcaaaaac
     2401 taaacaagtg aatacaactt cactaaatgt aaggagtgga ccaaatacaa gttatggttt
     2461 gttaggcaag ttgccaaaag gaagtaaagt agaagtaata tcagaaggta atggatggtc
     2521 caaaataaag tataatggaa aagatgccta tgtatctagt atgtacttat cggatgtaag
     2581 tcaaagtaat tcagacaatt ctagtcaaag caatgataaa aagaatactg ataaggtcgt
     2641 aaatacagct tctttaaatg taagaagtgg accaggttct acatatagta agttgggaaa
     2701 agtttataaa ggaagtaaag taactgtact atcagaaagt agtggatggg ctaagattaa
     2761 ttttaacaat aaagaagcat ttgtagtagg caattattta tctacttcag cagatacttc
     2821 aaataataac tcaaatagta actctgataa cagttctaat agtaatggca ataattcttc
     2881 atcatcaggt caagtaaatg gtatgtcagg tataagtggg gctaaaattg attataaatc
     2941 tttgagttat actttagaat ctcatataag taaacaagtt gagaaagcag catcaggagg
     3001 aaatgtgata gctccaagta ataggaaaag cactccaagt cctgaattta gtactttttc
     3061 agctcaaaga acaagctcat ttgtaaatgc aagttcaagt gatatagaat attatttaaa
     3121 tcctaaaaat tttacaaata ctactaaagg tatgatgcag tttttaaaga ttaacagtta
     3181 tagagatggt atttcagaat caagtcttaa ctcgtatctt aatggtttat cttctagtgt
     3241 atttaaaaac cagggagcag cctttataaa tgctgccaag aagtataata tagatgttgt
     3301 atatctagta tctcatgcta tgtgggaaac tgcttatggt aagtctacac ttgcccaagg
     3361 tcaaacatta acatcttata agggacaacc tcttagtaag ccagttaaag tatacaattt
     3421 ttttggtata ggagcaatag ataaaagtgc taatgtttca ggtgcagaag cagcgtattc
     3481 caatggctgg actagtgtag aagctacaat agatggctct gcaaagtgga tatcacaaaa
     3541 ttacgtaaat agttctaagt acaatcagaa tactatctac aagatgaagt ggaattatga
     3601 ttatacatgg catcagtatg caactgatgt gaactgggct aatggaattt ctggaattat
     3661 ggaaaattta attggtcttt atggaggagg aagtagtttg gtttttgaag ttcctcaata
     3721 caagtaatac ataaataaaa aatatagact atgggtagtt ttcattttat ttaaattatc
     3781 tatagtcttt ttgtatataa tttataatct gtaaatttaa tgctaacaaa acttttatgt
     3841 tataatatta attctaagta aatataagtg agtaataggt agaaatagat aaaaagtggt
     3901 aataatatat ataaagataa ttttgagaaa ggaggatata tatggaaagt attaaggaat
     3961 atttggataa gttagaaatt aataatagtg gtcttggaaa gcaacttaaa gaagtttata
     4021 taaatagagt agtttatttt aaggaagata agatagttta tttttatcta acttctaaag
     4081 atattgtttc tcatgaactt ttagataaat ttaaagaaga attgatgtat aaactcgatt
     4141 attttaaaga tatgaggatg aagataagat ttactggtct tgaaagaaaa tccaataaag
     4201 atgttataaa aaaatattgg aataatatat tgtatatatt aaagtatctt tgtccatcta
     4261 ttgcagggtg gtataaacaa gtagaatttc tttgtttaga agaagaatta aaaataaaac
     4321 ttccaaaagg tatattttat gagcgattaa tgaaaaaaaa tgtaacttat gtacttaaaa
     4381 ccgtgcttag cgaagagttg ggattagatt taaatataac aatagaaaaa gctgttgatg
     4441 aaaaggtaaa taaagaaaga ctcataagaa tcaatgatag agaaatggaa gaaaaaatta
     4501 gggctttaga aattggaaaa gttaataact gtgaagaaaa tgaagaggaa agttatgtca
     4561 ttaagtctga tgtagatgaa aatttaatct atggagataa tgcaaatgct atgattgaaa
     4621 atatagttga attaaatgca tcttctggaa cagttgcagt tgttggagat atatttgatg
     4681 ttgacactaa agaacttaaa aacggaaaaa tacttatgat agcttcaatt acagattata
     4741 ctagctcaat aagctgtaaa ctttttttaa cagatacaaa taaggatgga gtacttgaaa
     4801 atgttaaaaa aggagcatac ttaaaaatca agggtgacat agtttatgat acatatcaaa
     4861 gagaaattag tatgatgatt agtgggataa gaaaagaaac tagaatagag agagaagata
     4921 aatcagagga aaaaagagta gaactccatg cacatacaca aatgtcttct atggatgcta
     4981 tttgttctgt taaaaagctt gtagagcgtg cagccaaatg gggtcattct gctattgcta
     5041 taacagacca tggtgttgta caaggatttc cagatgctat gaatgcaggt aagtcaaata
     5101 atataaaaat actttatgga gtagaagggt acttggttga agatgattct ctcattatag
     5161 aggatgcaaa tgataaagaa ctttcgcaga catttgttgt atttgacata gagacaactg
     5221 gtttttctaa tacaaatgat aaaataactg agattggtgc tgtaaaaata gaaaacttta
     5281 agattgtaga tagatttagt gagttaataa atcctgaaaa agatatttct tataagatac
     5341 aggaattgac aggaataact aatgaattaa ttaaagacaa gccaaccatt gaagaagttc
     5401 ttcctaagtt tatggaattt gttggagata gtgtattagt agcacataat gcagagtttg
     5461 atactggatt tatatcgcaa aaatgtaggg aacaaggctt gttatataat aacaaaaaag
     5521 tagatacact tatgttagca agagtaatgt tgccaaactt aaaaagacat agattaaatg
     5581 tcgttgcaaa agaacttggt attcctcttt taaatcacca tagagcagtg gatgatgctg
     5641 aagctacagc acttatattt aataagtttt tgcagatgct tacagaaaaa ggtgcaaaaa
     5701 ctctaagtga tgtaaacaat atacttggaa agattgatta cacaaagctt agcacaaatc
     5761 atataacctt gatagcaaaa aattctatag gtataaaaaa tttatataaa ataatttcag
     5821 atgcacatgt aaatcatttt tttagagcac caagaatcct tagaagtgta ttaaatgaat
     5881 ataaagaagg attaataatt ggttcggctt gtgaagctgg ggtggttttt caagctgtaa
     5941 agaaaaacgt gtctgatgag gaaatgaaaa aaataataga tttatacgat tatattgaag
     6001 taatgcctat agataataat agatttatga ttgataaagg agaagtaaag gacgaagaag
     6061 agttaaggga gttgaatagg aaattaattg atacagccaa aaaatttgac aaaattccag
     6121 tagctactgg ggatgttcat tttttagaca aacatgaagc tgtcttaaga aaagttctta
     6181 aatattctca aggttttaaa gtagatgaag aagaaactta tttacatttt agaacaacag
     6241 atgaaatgtt ggaagagttt agttatcttg gagaagaatt ggcatatgaa gttgtagttg
     6301 aaaatagtaa tctgattgcc gatatggttg aagatataaa acctatacca gatgatactt
     6361 atcctccaat tatagagggc tcagatgtgg agttaagaga aatgtgttac aaaaaagcca
     6421 aacgaatcta tggagaccca ataccagaaa tagttcaaaa aagactggat agagaattaa
     6481 attcaataat aagcaatggg tatgcagtta tgtatattat agctcaaaaa ctggttgcaa
     6541 aatctctttc agatggatat ttagttggtt caagaggctc agttggctca tcttttgcag
     6601 ctactatgag tgatataaca gaagttaatc ctcttccagc acattatatt tgtgaaaacg
     6661 aagactgtaa atattcattc ttttatgaaa taggtgaatg gggctcaggt gttgatttac
     6721 cagacaagga ttgtccaaag tgtggaagaa aacttgctaa aaacggtcat gatattccat
     6781 ttgaggtttt cttaggtttt gaaggtgata aagaaccaga tatagactta aatttctcag
     6841 gaaattatca gccaacaatt cataaataca cagaagaact atttggagag gggtatgtat
     6901 atagggctgg gactataggt actgtagcag aaaaaacagc ttttggatac gcaagaaaat
     6961 atgtggaaga aaataatata agtgtaccaa atgcagaagt acttaggctt tcaaatggat
     7021 gtactggagt aaaaagaacc tcaggacaac atcctggtgg agttatggtt ataccagact
     7081 ataaagatgt atacgatttt acacctatac agtatccagc aaatgatact tcttgtggtg
     7141 ttataactac tcactttgat tatcattcaa taagtggtag aatacttaaa cttgatatac
     7201 ttggacacga cggtcctacc attataagga tgcttgaaga tatgactgga ataaatataa
     7261 cagaaattcc tttagatgat aaagagacta tgtctctatt tacatctaca gaagcattag
     7321 gtgttacacc agaagaaata aactgtccta taggttgcct tgctgtacca gagtttggaa
     7381 ctaagtttgt acgacaaatg ttacttgaca caaaacctac tacatttgca gaacttgtac
     7441 gtatatctgg tctctctcat gggacagatg tttgggtaaa taatgcacaa gacttagtta
     7501 gagaagatat agttggatta aaagatgtta tatctacacg agatgacata atgaactatt
     7561 taatatttaa aggattacct cccaaaatga gctttacaat aatggagagt gtaagaaaag
     7621 gtaaaggttt aaaaccagaa cacattgaag aaatgaaaaa aaataatgtt ccagaatggt
     7681 atataggctc atgtaaaaag attaagtaca tgttcccaaa agcacatgct gttgcttatg
     7741 taatgacatc atttagaatt gcatattgta aagttcacta tccagaagca ttttatgcga
     7801 cttattttac gacaaaagtt gaggattttg atgcagattt aattgttaaa ggattagatg
     7861 ctataaaatc aaaaataaat gaaattgagt cactgggaaa tgatgcaacc acaaaagaaa
     7921 aaggtatgtt gaccgtttta gaagttgctc ttgaaatgta tgctagagga ataaaattat
     7981 taccagttga tatatataaa tcagatgcaa ctgagtttat agttgtagga gaaaaaacat
     8041 tacttccacc aatggcagca atacaaggtc ttggaggaaa tgcggctata aacatccaaa
     8101 atgaaagaaa aaatggtgaa tttatatcaa aagaagattt gagaaagaga acaaaaatat
     8161 caaaaacggt aatagaaact cttactaatc atggttcact tgagaatatg agtgagagaa
     8221 atcaattgtc attgttttaa gttagcccaa aaagttgcaa ataaaagaat tttatgatat
     8281 catagtatag aaaatagtat actttgcaga aactaagagt gagacagctg ttctcactct
     8341 tttattttaa atatattatt aaaaagctgg tttattttta ttaaatatat tttatttaag
     8401 attatataaa ctggtcattt acaattgaat aggaggaact ttaatgaaaa aaaatttaga
     8461 agctacaatt gaggagattg taactaaaat aacggatgaa catggatttg aaatggttga
     8521 tgttgaatat gtaaaagaag caggagaata ctacttaaga gtatacatag ataaagaaga
     8581 aggaatatct ttaaatgaat gtgaattagt tagtagagaa ttaagtccaa tactagatga
     8641 aaaagaccct ataaaagaaa attacttttt agaggtatct tctccagggc ttgatagagc
     8701 attaaaaaaa gacagagatt ttgttagata tcaaggtaga gatgtagatt taaaattata
     8761 taaaccatta aatggatgta agcaatttga aggtgaactt gtaggattaa cagaagataa
     8821 taatataaaa atcattgcaa atggtaagga aatggaattt aatagaaaag atgttgctat
     8881 tgtcagatta gcaataaagt tttagtggta tattaaatag ggaggatata aaaaatgaat
     8941 catgaattta tggaagcatt agatgaatta gtagttgaca gaggtataga taaagaaata
     9001 cttatagata ctatagaaca agctctttta actgcataca agaaaaactt tggttcagca
     9061 caaaatgtta gagttgaatt tgatagagac aagggagata taaaagtatt ttctcaaaga
     9121 gttgttgttg atgaatcaga tttatatgat actttcttag aaatagagtt agcagatgct
     9181 agagagataa accctaatta tgaattaggt gatattatag aacatgaagt tacacctaaa
     9241 aactttggaa gaattgcagc tcaaacagca aagcaagtag ttgttcaaag aataagggaa
     9301 gctgagagag aaatagtgta taatgaattt atagaaaaag aaaatgaaat agttacagga
     9361 gaggttgcta gagcaaataa aaatgttgtt tatgtaaatc ttggaagaat agaaggtata
     9421 atgactcaaa gtgaacaaat accatttgag aattataaag caggtcaaaa aataaaattc
     9481 tatatacttg aagttaaaaa gactaacaaa ggacctcaaa tagttgtatc aagaagtcat
     9541 ccgggtctag taaagagatt gtttgaagaa gaagttcctg aaatatatga aggaatagtt
     9601 caaataaaat ctgtagcaag agaagcaggg tcaagaacta aaatggcagt aagatctata
     9661 gatgaaaaaa tagacccaat tggagcttgt gttggaccaa aaggacttag agtaaaaaat
     9721 atagtggatg aacttggcga tgagaaaata gatataataa aatatagtga agacccagca
     9781 gagtttatat cagcagcact tagcccatca aaggttgtta gagtagatat agatgaagat
     9841 gaaaagtctg ccttagtaat cgttccagat tatcagttat cgcttgctat aggtaaagaa
     9901 ggtcaaaatg caagactagc agcaaaattg actaactgga aaatagatat taagagcgaa
     9961 tctcaagcag aacaagaaat gttaaatctt gaaaattcca aagaaataga agctgaggat
    10021 gttgaagctg aatttatcga ccaagaagag ttagatactt cagaggataa ttttagtgaa
    10081 gaagagtaag gagggatgat tttgaaaaaa gtaaaaaaag tgcctcaaag aaaatgcata
    10141 gcatgtcaag atagagactc taaaaaagag cttataagaa tagttaaaaa taaagaagga
    10201 aaaatatttt tagacccaac aggaagagcc aatggaagag gagcatatgt atgtaaaagc
    10261 tctgaatgtc taaaaaaagc tataaagagt aaagcattaa ataaagcttt taaaatagat
    10321 gttcctgatg aagtctatga taatctactt atggagttag aagaatatga aaaataataa
    10381 agaaaaagta ctttcgttct tagggctcgc aactcgtgca gggaaaatag tgtctggtga
    10441 tgattcaact ttgcttgacc ttaaaaaagg aaaagtaaat ttgattttaa tcgcagaaga
    10501 tgcatcaaat aatactaaaa aactttttaa agataaatca acttttagaa atattccata
    10561 tctatttttt tcaacaaaag aggaaatagg atttgcaata ggaaaatctc caagagcagt
    10621 tgtaggaata aaagatgaaa atttttctaa aaagataatt gaactaatag aaatttaaaa
    10681 taaaataggg ggtgatcttg tgtcaaaaac aagagtatac caaatagcag aagaattaaa
    10741 tatatcaaat gaagagctta taaataagtt agctgaatta gatataaatg taactgacaa
    10801 agattcagta ttagaaggtg aagagttaga attagcactt gaaatgttag gtgaagattt
    10861 atctcaagaa aatggaaatg ttatagaaat agatggaaag ttgacagtac aagtattagc
    10921 aacgaaatta gataaatctc catcagaaat aattatgaag cttatgaaaa tgggaactat
    10981 ggcaacaata aatcaagaga taagttttga gattgctgct ttggctgcca aagactatgg
    11041 atttgaatta acagtagctg aaagtgatga tacagaagct ttagaaatag aagctttaat
    11101 ggaaattgaa gaagataaag aagaggattt aaaacctaga ccaccagttg ttacagttat
    11161 gggacatgtt gaccatggta agacatcttt actagatgca ataagaaaaa ctgatgtgat
    11221 ttcaggtgaa gctggaggaa taactcagca tataggtgct tcagaagtta aaatcaatgg
    11281 acacaaaata gtatttttag atactccagg acatgaggct ttcacatcaa tgagagcaag
    11341 aggagcacaa gttacagata tagcaatatt agtagttgca gcagatgatg gtataatgcc
    11401 tcaaacagtt gaagctataa accatgctaa agcagcaggt gtacctttaa tagttgctat
    11461 taataaaata gataaaccag gtgcaaaccc agataaggtt aaacaggaat tagcagacca
    11521 aggattatta gttgaagact ggggtggaga agtaatagca gttccagtat cagctaagaa
    11581 aaaagaagga atagatacac ttttagaaat ggtattattg gttgctgaaa tggaagagtt
    11641 aagagctaat ccaaataaaa gagcagtagg aactgttata gaagctgagc ttgataaagg
    11701 aagagggcca gttgcaacag tacttgttca aggtggtact ttaacagtag gtgacccaat
    11761 cgttgcaggt gttgcatgtg gtaaagttag agcaatgata aatgcaaaag gtaagagagt
    11821 aaaaacagca ggaccatcta cagcagtcga gatattaggt ctatcagaag ttccacaagg
    11881 aggagaccaa tttgtcgaag ttccaactga taagattgct agaagtgtag cagcaagaag
    11941 acaacaaata gttagagatg aaatgttaaa atcaactcaa agattatcac tagatgctct
    12001 atttagtcaa atgagtgaag gttctataaa agaccttaat atagttataa aagcagatgt
    12061 acaaggttca gtacaggcag ttaaacaatc tttagaaaaa ttatcaaatg aagaagttca
    12121 agttaaagta atacatggtg gtgttggagc tgtaacagaa tcagacatat tacttgcagc
    12181 agcttctaac gcaataataa taggattcaa tgtaagacca gttccaggtg cagaatctct
    12241 aggagaaaaa gaaaatgttg atataagaac atatactata atatacaagg caatagaaga
    12301 tatacaagcg gctatgactg gtatgttaga ccctgaatat gttgatgaag aaacaggaaa
    12361 agctgaaata agagaaatat ataaaatatc tggtgttgga acagtagctg gatgttatgt
    12421 gactaatggt aagatattta gaaattgtaa agtaagatta gttagagata gcataataat
    12481 acatgaaggt gagcttgcag cacttaagag atttaaagat gatgtaaaag aagttaattc
    12541 tggatacgaa tgtggtatga gttttgtaaa ttataacgac ataaaagaag gcgatatagt
    12601 agaagcttat ataactaaag aagtagaaag aaaattataa attcgtatca aagaaaatca
    12661 gttagtaaag aaggtcgatg gtatggcatc atataataga acaagaagga tagcagaaga
    12721 aataagaaaa gtcgtaagca ctatgcttat aaatggagta aaagacccta gaataacatc
    12781 tatggtgagt gtaactgatg tagaagttac aaatgaccta agatatgctt atgtatatgt
    12841 aagcattcta ggtggagatg aagaatccac actaacaggt cttaaaagtg ctggaggata
    12901 tataagaaga gaagtaggaa aaaatataaa acttagatat attcctgaaa tagtatttaa
    12961 acttgatgat tcaatagaaa aaggaatgta tatggatagc cttataaaaa gagttaatga
    13021 aaaaaatgct caacaaaatg aggatgaaaa ttatgatgaa taatatggat agtattataa
    13081 actatattta tgagggtgac tattttatag ttacctctca tatcagtcca gatggagaca
    13141 atattggttc tacactttca atgtattata cattgaagaa tttaggaaaa aatgtatatt
    13201 atgtacttga tgatgaagct ccattaaatt taaggttttt ggttgaagat gtaaaaatct
    13261 ataagtctaa tgaatttaat atgaagaact attctctaat tgcacttgat tgtggggata
    13321 agatgagaat atgtgtgagt gatgaaatca aaaataatgc atcaaaatta atctgtattg
    13381 accatcatgc tagtaatgat tcatatggag atttaaatta tgtagatatt gatgcatctt
    13441 ctacatgtga attagtttat aatttattag ttagatttca gcaaataaaa gatattaata
    13501 ttataaatga agatatagca acatgtttat atacaggtct tgtaactgat actggaaact
    13561 tttcgtattc aaatgtacat gcaagtagct ttgaaatggc aaaaaattta cttgttttag
    13621 gtgcacaaaa aaatactata atacaaaata tatatcaaag caattcttct ggttattaca
    13681 aattattagg agaagctcta aagggtttgg agatatttga ttcaaaggta tctagtatag
    13741 ttttaactca agacatgatg aatagaaata atattagctt taatgatgtt gatggaataa
    13801 caacatatac aagagacata aaaggtatag aagttggaat tttatttaaa gagaaaaaac
    13861 aaaatgaaat taaagtgagt tttaggtcaa aaaattatgt tgatgtaagt gaaatagcca
    13921 agttatttgg tggtggtggt catgtaaggg ctgctggatg tactattaga gatagtattg
    13981 ataatgcaaa gaaaatggta ctagaggcag tgttaaaatc aatttaaggt gacagtaaaa
    14041 tgaataagat tataagtata ttgaaaccca caggtatgac atctcatgat gttgtaagta
    14101 gagtgagaaa gattttaaat ataaaaaaag ttggtcatac aggtactctt gacccagatg
    14161 cttcaggtgt tttaccaatc tgtataggga aagctacaaa agtatgtgaa gttattttaa
    14221 acaaagataa atcgtatata tgtgagctta ctttaggtat aagtacagat acttatgatg
    14281 cttcaggtga aattttaaaa aaagttgatg attttaaatt tagtaatgaa gacatagaaa
    14341 gagcttttga cacacaaaga ggagagataa atcaacttcc tccaatttat tcagctttaa
    14401 aggtaaatgg aaagagaatg tgtgatttag taagaagtgg tagacaatct gaaattactt
    14461 taaaaacaag aagagtaaat ataaaagaca taaaaatact aagtataaaa ggtaataaag
    14521 taatgtttta tgttgagtgt tctaaaggga cttatgtgag aagtatttgc catgatatag
    14581 gagaatatct aggttgtgga gctcatatgt cgtttttgaa tagaacatct tctggtaaat
    14641 ttgacttaga taattcaata acattagaag aattagaatt attttatgaa aataaaactt
    14701 tagacaaata cttatatgat atagattatg tccttgattc gtttaattat gttgtattaa
    14761 accctaatgc tataaaatat tatagtaatg gtggctcaat agatgataaa aggtttttaa
    14821 agaataattt cgataaagat gatgaatttg taagagtata cagtacagat aatttcttag
    14881 gattaggaaa gttatcaaaa cacaataata caataagtgt taaaagtgat aagatgttca
    14941 tataaccaat gtatttaaaa aaacaacaaa agagatagta ctataataac ttctatagtc
    15001 tatctctttt acttatgcta taatagtttg tgaataagtt aagagtaaat tatattttag
    15061 cggggaacat ttattatggt gattataaaa tctataaaag aaatagcaaa cataaaagaa
    15121 agtgtcatta ccataggaaa ttttgatggt gtacacaaag gacatcaagt tcttatagga
    15181 aaaacagttg aacatgcaaa aaaagaaaat atacagagta ttgtatttac atttgcaaat
    15241 catcctgtaa attactttag accaaactca acaaaaaata ttataagcaa tgatgataaa
    15301 ataaaattat tagaagattt gggaatagat atagttgtaa tcataccatt tgatgagtat
    15361 atgactaaaa tacctgcaaa ggatttcgta gatgagatac tagtaaaaaa attaaaagct
    15421 aaaaagatag taattggaca tgactttaca tttgcaaggg caaaagaagg caatgttaat
    15481 ctattaaaaa gcttagaaca tgaatttggt tttgaggtag aagtaataaa acccatcaaa
    15541 atcaatgatg taagagtgag tagtacttat attagaagtt tagtatccca gggtgatatg
    15601 gctaatgtca aggaatattt gggtagaaat tacaagttag aaggttgtgt tatacattct
    15661 aaacaccttg gaagaactat aggattccca acggcaaata ttgaccttaa gaataatatg
    15721 ctcgttccaa agagaggtat ttatgcttct attgtacata ttggagatga gacttatttt
    15781 ggtgctacaa atgtaggcta taacccaaca gttaatggaa aaactttgtc tattgaaaca
    15841 aatattttag agtttgatag agatatttat ggggaaaata taattgttga atttttggaa
    15901 agaataagag atgaaagaaa gtttaattca atagatgact taaaaaatca attgtataat
    15961 gatacaaatt atgtgtatga aaactatgtt tgcaaaaaca agtaatgtat gataaaatga
    16021 ttaaggaaaa taaccattac tcggcatttg aatactccaa tcaatgctta gtaataggcg
    16081 tttctaaaaa aatacggagg taatctaaat gataaacaaa tcagaaataa taaaagaata
    16141 tggaagagct gaaggggata ctggttctcc agaagtacaa atagctttat taacagctag
    16201 aataaacgag ttaaatggac atttaaaaac tcataagaaa gaccaccatt caagaagagg
    16261 actattaaaa atggttggta gaagaagaaa cttattagct tacttaaagg aaaaagatct
    16321 tgaaggatat agagcattaa tagctaaatt aggattaaga aagtaatttc attctttata
    16381 aaaaagcagg tatagtgtat acctgctttt ttatagttaa ttcattataa agtaaaatag
    16441 ttaataataa aagtagttta tatgttataa aaaattataa aatgaataat attgggtaag
    16501 aatattaaaa tagtatacaa atacataatc aatttaaaag gaaagacagg ataaatatag
    16561 aaattttata acaggataat tagttggaat aaggaggata ttattttgaa aataaagtta
    16621 gtatgcgata gtttgtgcga tattccagat gaaatatcgg aaaaagatta tatagagata
    16681 gttccactta cagttatttt taatgatagg gaatatatag aaggagtaga tataaagaaa
    16741 gaagagtttt ataaaaaagt aaaagaaatc aagcaaatac ctaaaacttc acaagctaca
    16801 tatatggagt ttaaagaagt gtttgataag ttcataacag aaggttatca tataatctgt
    16861 atgacagggg ctgcaaacgc atcaggaact tttcaaagtg ctatgatagc taaaaatgat
    16921 gtaaatgaaa atgaaaaaat acatatattt gatactagaa atttatctct aggtagtgga
    16981 caatatgtta taaaggcatg tgaacttctt gaagaaggac tgggatttga agaaattata
    17041 gatgaattag aaaatactcg cagtagtgta agattgcttt ttgcaccata tacacttgat
    17101 tttttaaagc agagtggaag agttccagtt gcaactgctt tgataggaaa tatgttaaat
    17161 ataaagccta tatttttctt tgataatggg gaagctaagt tggtaaataa ggttagagga
    17221 ataaagaata tagcaagtaa attggtcgat ataattttag aaatgaatga agggtcttta
    17281 gaaggaaaaa tcgtcacaat aggttgtgga gataatttac acgattgtga aatattaaaa
    17341 gatgaagtaa ataaaagaat aaaagcaaga agagtattat ttactcgagg aggagtttct
    17401 atttgctctc atacaggacc agatatattg gctataagtt gttctaatta gtagtaatat
    17461 aaggtttttg catatatatg tgaagataat tttatattgt taatttatag tatttttgat
    17521 aatatagtaa atagagtgat atacgtgttt tttaataaga ggaggacaag cattaatgtt
    17581 tgaacataaa atatttaaaa tggattttgc aggaagagaa ctttctgtag aaataggaaa
    17641 gatttgtgaa atggctagtg gtagttgtat agttcgatac agtgatagta tggtaatggt
    17701 aaatacaact aaatcagcca aacctagaga cggaatagac ttttttcctt taagtgtgga
    17761 ttatgaagaa aaattatact ctgtaggtaa gatacctgga ggttttttaa agagagaagg
    17821 caagccttca gagaaagcta tacttacatc aagattgata gatagaccaa taagaccttt
    17881 attcccaaaa ggatttagaa atgatgttca ggtagttgca acagttctat cagtagacca
    17941 agattgtaca ccagatatag tagcaatgat aggttcttca atagcccttt ctatatcaga
    18001 cataccattt aatggaccaa caggttcagt atgtgttggc cttgtagatg gtgcatttgt
    18061 tgtaaatcca aacgcagagc aaagagaaaa aagttcaatg catctagttg tttcaggtac
    18121 aaaggaagca ataatgatgg ttgaagccgg agctgatgaa gttccagatg aggtaatgct
    18181 tgatgctatt ttatttgctc accaagaaat aaaaaaaata gttgagttta tagaaggtat
    18241 agttgcagaa gttggaaaag aaaaaatgcc tgttgaatta taccatgcag gagaagaaat
    18301 aactcaattg gttcgcgaat ttgcaactga taagatgaaa aaagcagttc agacttttga
    18361 aaagttagag agaatggaaa atatggatag agttaaagaa gaaactttag ctcactttga
    18421 agaaacttta gaagattttg aagattttgt tggagatata gaagaagtat tacaagatat
    18481 aataaaagaa gaagtaagaa aacttatagt acatgaaaat gtaagaccag ataatagaaa
    18541 acttgaagaa ataagaccta tatggtgtga aactggcatg atacctagag ctcatggttc
    18601 agccatattt actagaggac aaactcaagt acttaatgtt gctactttag gtgctttagg
    18661 agatgttcaa aaactagatg ggttagatga agaagaaaac aagagatata tgcatcatta
    18721 taatttccct gcatatagtg ttggagaagc tagaccatca agaggtccag gaagaagaga
    18781 aataggacat ggtgctttag cagaaagagc tttattacca gtaataccat ctcaagaaga
    18841 atttccttat gctattagat tagtttctga agtattaagt tcaaatggtt ctacatctca
    18901 agcaagtgta tgtggttcaa cattatcttt acttgatgca ggtgtaccta taaaagatat
    18961 ggttgcaggt atagcaatgg gacttataaa gcatgatggt aaggttgcag tgctttcaga
    19021 tatacaaggt atggaagacc atttaggcga tatggacttt aaagtagctg gaacagagta
    19081 tggtataaca gcaatacaaa tggatataaa aattgatggt atagataaag aaattctaca
    19141 acgtgcttta aaacaagcta aagagggtag aatccatata ctaggagaga tgagaaaaac
    19201 tatatctcag ccaaaaccag aactttcacc atatgcacct aagattgtta aaatgcaaat
    19261 aaatcctgac aagataaaag atgtaatagg acctggtgga aagataataa ctaagataat
    19321 agacgaaact ggagttaaga tagatattga acaaactggg gaagtattta tatcaggtat
    19381 tgagattgat atgattaaga aagctcaaga acttataaat aatatagtag ttgaaccaga
    19441 agtaggaaaa acttataaag gtaaggtaag tagaataatg aactttggag catttgttga
    19501 gatacttcca ggaaaagaag gattacttca tatatctcat atagctcatg aaagagttgc
    19561 caaagttgaa gatgtattga atatagggga tgaagttgaa gttaaagtta cagagattga
    19621 tgaaaaagga agagttaatt tatcaagaaa agttctttta ccaaaaccag aacataaaaa
    19681 taaataactt attatataat taaaagcaaa gtcttaggat tttgcttttt taaatacaaa
    19741 aaagtagata attttaaaat gatagttatg gatggcttat agttcttttt ctgtcagtct
    19801 ttactcttat aaagaagcta tttaaaagct gattaataga aagctttatt gatatatata
    19861 gaatcactta agttatattt agtgcataaa cagagcttat ttttaatagt attaaataag
    19921 aaacaatttg tacataatta gggggagttt taatgataat gatggtactt aataagagta
    19981 agctcaaaaa aatcataata ctaattttag tagtgttatt agtattatca ggaggggtaa
    20041 tggtattaaa gttatttaac aacaagccag tatttaattt atttaatggt gtagatttac
    20101 catatgaaag aggtacaaag gataaaagtg gatatgttgc attaacctgt aatatagacc
    20161 ttggatggga aacagagtac gtagaatcca ttttagaaac acttaaaaaa gaaaatgtaa
    20221 agattacatt taatgtaact ggaaaatggg cagaaaagaa taaggatgaa ttattaaaga
    20281 taaaaaaaca aggacatgaa ataggtaatc atggatataa acatttagat tattctacat
    20341 tatcatatga agacaattat gagcaaatag agacctctaa aaagataatt gaagaaatta
    20401 taggtgaaaa aactaaattt tttcaagcac cagcaggttc ctttggtcca gaaacagtaa
    20461 aggctgctaa agcattaggg tatacttcta tcaaatggga tgccgatact atagattgga
    20521 aatacaaaga ccaaccagag gtaatcattg atagaatgaa gaaaaaagac attaaagata
    20581 gtagtataat cttaatgcat ccaaccaacg caacgacaaa atgtatagat gatattatag
    20641 ctattgtaag agaaaaagga ctaaaagcag gaaaacttag tgatgtattt aagtagacaa
    20701 agtataaata tttggttaag attgtataag ttgtagttga attaattaaa agactgactt
    20761 ataatgtaat acatataaaa aataaatata attaaaacat agtgttttca ataatccaat
    20821 atgaaacaaa gaataaattg ttaggagaat gtaatgtata agacaaaaat actggaaaat
    20881 gggcttacaa taattggtga agaaatacca tatctaaagt caataacatt aggtatttgg
    20941 ataaatgcag gctctaggat agaagaagca caggtgagtg gaacttctca ttttatagag
    21001 catatgatgt ttaaaggaac caaaaacaga acatcaaaag agattgctag ttcaatagat
    21061 aatttgggtg gtcaaataaa tgcatttaca agtaaagaat gtacatgtta ttatgtaaaa
    21121 ctaatagatg agcatataga tacaggaatt gatgtattga gtgatatgat actaaattct
    21181 aaatttgata aaaatgatat agataaggaa agacttataa tattggaaga acttaagatg
    21241 tatgaagact ctccagatga cttatcttat gatttattag tggaaaatat atatgctaat
    21301 gatggattag gaatgaatat aattgggaca aaagaatctc tatacaatat aactagagaa
    21361 tccatgttag aatacttaaa taagtattat attccaaata acgctgttat ttctatagct
    21421 ggaaacttta actttgatga tatggtggag aaaatcaaat caaaatttgg acactgggaa
    21481 aagaaaaatt tgagtataga cataagtgag gctaagttta atccttgctt tatatcaaaa
    21541 aataaagata ctgaacaggt gaacttagct atgtgcctaa aaggtattcc ttttgaaaat
    21601 gatgaagaag tctattcaat ggcagtggta aataatattt tcggtggaag tataagctca
    21661 aggctttttc agaaaataag ggaagaaaaa ggtttagtat attcaatata ctcatcacag
    21721 acattatata ggaaatgtgg tgaattaggt atatttgcaa gcatgagtac agaaaattta
    21781 caagatgtat ataatttaat aaagaaagaa attgaaaata taagagaaaa ctatcttaca
    21841 gaaaaagaaa tatctgaaag caaggaacag ttaaaaggaa attatatatt ggatttagaa
    21901 agtactagca gtagaatgat gtctactggt aaatctatgt tacttagtaa aaaagtaaaa
    21961 actacagatg aaatacttga atgtataaat agtgttaata ttaattcaat aaagaaggtt
    22021 gttgataagg tattcaatat cgaaaatatt ggtacatgta tagttggaag agatgtagaa
    22081 aagattctac acttggatta atattgaaaa aagtaaatct ctggaggtat aaaatatgaa
    22141 tttatctgaa atagcaggta aggaaatagt taacttggtc acaggagaaa gattgggggt
    22201 cattggagaa tgtgacctta tactagatga aactacaggc ggtatattag cgcttctaat
    22261 tccaaaagaa aaaggatttt ttgccagaag aaaagataag tcatttttag aagtaccatg
    22321 gagaaatgta aaaaaaatag gtaatgacat gataattata gaatatgagg gatatattta
    22381 ggaggaaaaa atgagtattt tagtgcaaaa gtttggtggg acatcagtag aatcatatga
    22441 aaaaatgaat gaagtatgta agatagtaaa agcatacaaa aaaaatgatg aagaattaca
    22501 attagtatta gtagtttcag caatgggaag aaaaggagct ccatatgcta cagatacact
    22561 tataaatttg tgtagtgcag taaatgatga gccaagtaaa agagaacttg atttaataat
    22621 gtcttgtgga gaaataatat caggaacaat tcttgctaat ttattgaatg cacaggggat
    22681 agattctgtt ttctttactg gtcagcaagc aggtataatt acaagtgatg aatattctaa
    22741 tgcaaagata aagtatataa atcctaaaaa ggtaaaaaga gctttagatg atggtaaggt
    22801 tgtagttata gctgggttcc agggtgtaac agatgatgga gaaataacta ctcttggaag
    22861 aggcggaagc gatacatcag cagttgctat tggtaaagct ttagaatgtg aaacagttga
    22921 aatttacact gatgtggatg gtataatgac tgcagaccct agagtagaac caaacgctaa
    22981 agtattgagt tttatagact atgaggaagt attccaaatg gctgacaaag gtgcaaaagt
    23041 aatacatcca agagcagtac agttggctaa aagtggaaat ataactttag ccataaagaa
    23101 tacaatgaat ccaacttttg aaggtacaaa aataggttct ttatgtagac acttagaaga
    23161 caatatagaa tatgaacaag aaagagattt taaggttgct gttgcaaata aagatagcgt
    23221 agcacaagtt aagattaagt cagcagaaga agtgtttaca gaagtattaa atgagataga
    23281 aaagaaactt ataactatag atatgataaa tttctttata tcagaaaaag catttgttgt
    23341 agaagatgct gatataaaat cattaaagga aatactagat aaatttgaat tagattatga
    23401 agtaaataga gactgtgcta aggtaacatt gatatgttca agaattgctg atgaaatgtc
    23461 tggtatcatg tcaaaggtag ttagaggatt atcaaaagca ggggtatccc ttttacaaac
    23521 atcagactca aatatgacta tatcttgttt agttagtgaa gaagatatgc atacagcagt
    23581 acatgctata catcaacaat tttatttaaa ataaactgta taatacaatt attttttaat
    23641 caaatttaag aatttcatat atatcttaat atatttgatt tatttggtaa aactaaatta
    23701 aatataattt tgataggagt ttttttatga aagattatag agaagataat gaaagatttg
    23761 ataaagataa ttgtagtgaa gaatctagtt gcgaagagag agaatgtata aagcaatttg
    23821 gtaccaatga aatgccacct caacctccta aagatattca atgtataact ataattggag
    23881 aaattgaggg acattttata ggaaatcctc aaaaaaaagc tacaaagtac gagcatataa
    23941 ttccaatgct atactcaatt gaggaaagta atgatgttaa aggagtgtta gttgttttaa
    24001 acactgttgg aggagatatt gaagcaggtt tagctattgc agaactttta aacagtacat
    24061 ctaaaaaagt agttactctt gttctaggag gaagtcatag tataggtgtt cctcttgcta
    24121 cagcaggaga ttactcattt atagcaccaa cagcaaccat gattatacat ccagtgcgaa
    24181 ctactggtct tgtgattggg ataaatgaga cttttgagta ttttaaaaag atgcaagata
    24241 gaataattca atttataata agaacatcaa atatcaaaaa agatgtactt gaaaaactta
    24301 tgcatgaaaa agatgaactt gtaagtgatg ttggtagtgt attaatagga aaagaagctg
    24361 tggattatgg tcttatagat gaagttggag gactaaaaga agcacttaaa aaattaagag
    24421 agcttataaa agaatctgag gaagaaaaaa ataatgatta aaatttgttt aaataataaa
    24481 atttatgata taatgatgta ataaagtagg agtgttagtg ttaaggcatt agcacttttt
    24541 gctgtgaagg ggtaaaactt gctaaaaata aggagtgata ttaatggcca cgaaaaagaa
    24601 gaaaaaaaag aaggtaaata ccttaagttt taatgcagaa tatcataatt taataacaat
    24661 atttataggt gtatttttat tatatagttt aaattctaat tctatgggat ggatacctgt
    24721 gttaatgcaa aatcttttta agggactatt tggagggctt tcaatagcaa taccatttat
    24781 agtaattata acaggacttt taggattttt tgatggaaat gaatatatat ataggctaag
    24841 aaagactaaa ctatactaca taattatttt atttatattt gtattttatg gattattaaa
    24901 tgcaggaact cttcctgtag atagcccact aaaaggtaac atgtttgatg atgttatgaa
    24961 attgggtgta agtggacaag gtagtggttt gatagcaaca actattgcgt attatatgag
    25021 taaaatattt ggtatagcag gtggatggtt aataagtatc ttcgcactta tactttctgt
    25081 aatgtttata tttaatattt ctataaaaga tttggtgtca aatgcaaaat caaaatctaa
    25141 agcatctaaa gacagcaatt taacgtttaa agataaaatt gcaaatatga aaaaatctgc
    25201 aatagatatg atgactgatg aagtagatga tactactata aataaaaaac caggtttctt
    25261 taaagggtta atgtcaaaag gtcttaataa tgatgatgaa gaagatgaat attttgaaga
    25321 agaaaatagt gatggtgtag atgataaaac tataaaaata gttggattta ataaggcaga
    25381 agatgagtat ttagaaatat tagaaggaac tcaaagtatg ccagagttag atgttttaaa
    25441 agaattacaa aaagctacca atgaaaatcc agttgtagat actaaaccag aaaaaaaagt
    25501 tgatatagct aaacctaatt taaatataga aaaaactcaa cctatgagta ttgtagcaga
    25561 gccagttaat gaggattatt caaactataa aaagcctagc attgagcttt taaataaagt
    25621 aaataagaag tctgatgaaa atggtaaaaa gaaagtgttg aaaaatgctt ctcttttaga
    25681 aaagacacta tcagattttg gagtggaagc aaaaattaat caagttacag ttggacctac
    25741 tataacaaga tatgaaatac aaccaagtcc aggagtcaag gtaagtaaaa ttgtaaattt
    25801 aactgatgat atagctttaa gtttagcagc taaaagtata agaattgaag ccccaattcc
    25861 tggtaaaagt gcaattggaa tagaagttcc aaatgaagaa gctcaaatgg ttggtgtgag
    25921 agaagttcta gaaagtgaag aatttaataa ttttaactct cctcttgcaa tgggacttgg
    25981 aaaagacgta gcaggaaaaa ttataattgg agatataggt aagatgccac acttactaat
    26041 agctggttct acaggttcag gaaagagtgt gtgtgtaaat acattaataa gtagtatttt
    26101 atataaggca aatcctgatg aagtaaaatt actattaata gacccaaaag tagttgaact
    26161 tgcaaattat aatggaatac cacatctatt gataccagta gttactgacc ctaaaaaagc
    26221 tgcaaatgct ttaaattggg cagttacaga gatgaataga aggtacaaat tatttgcaga
    26281 tgctcaagta aaagatgtaa caagttataa tgagaaagcg gaagaaaaac ttcctaaaat
    26341 agtaataata atagatgagt tagcagattt aatgatggca agtgcaaatg atgtagaaga
    26401 ttatatttgt agattagctc aaatggcaag agctgctgga atgcacttga ttgtagcaac
    26461 tcaaagacca tctgttgacg ttataacagg ggtaataaag gctaatatac catctagaat
    26521 agcatttgca gtatcatctc aaactgattc aagaacaatc cttgatatgg gtggtgcaga
    26581 gaagcttcta ggtaaaggtg atatgttatt ctatccactt ggtgcagcaa aaccagttag
    26641 acttcaaggg gcattcatat cagagagtga gtcggaaaaa gtaatcgatt ttgtaaaaag
    26701 tcaagttaaa gatggaataa aatatgaaga agatataatt gaaactatat ctaaggttaa
    26761 tacttcaaag ggtagtgatg aggatgagtt tttgagtgaa gcgatagaat ttgtcgtgga
    26821 aagtggtcaa gcatctgcct ctatgctaca gagaagattt aaaataggat tcaatagagc
    26881 agctagactt atagattcta tggaagaaag aggaataatt ggagcaagtg agggaagtaa
    26941 accgagaaag gtattaataa gcaaacagga tttgcagaat ttagaaggtg aatagaattg
    27001 tcagttataa aaatagaaaa agcacttaag ctagataaag taatatttgt agatgtgaga
    27061 actgaaggag aatatgaaga agaccatata ttgaatgcaa tcaatatgcc tctttttaaa
    27121 aataatgaac ataatgacgt tggaacaatt tataagatgc agggaaaaca tgaagcaata
    27181 caaaaagggt ttgactatgt atcgtataaa ttaaaggata tatatttaca agcagcagaa
    27241 cttgcattaa attatgataa tattgtcata tattgtgcaa gaggcggaat gagaagtggc
    27301 agtatagtaa atttattatc atcattaggt gtaaatgtat atcaacttga aggtggatat
    27361 aaggcttata gaaattttgt attagagtat ctaaggcatg taatggatac aaaaaacttt
    27421 gtatctgttc atggattgac tggtacagga aagactgatt tgttacattt attagatgaa
    27481 aaaggaatag atgtactgga tttagaaggc atagctaaaa acagtggttc tacctttggt
    27541 tttataactt ttgataaaaa gccaccttct caaaagaact ttgaaacaaa actatttgaa
    27601 agtatatttt tttcgaaaga gaattatata tttgtcgaga gtgaaagcaa acgagttgga
    27661 agtgttacag ttccaaatga aattttcgag gcaatgacac gagaaggaca tcatatatta
    27721 ttagaatgtg atattgaaaa tagagttgat agactttgta gagattatat ttatgataaa
    27781 gatgaaggta atatattgat tttaaaagaa tgtataaaca agtttagaaa aagattgggt
    27841 aataaagtag tagatgatta tattgattta ttagaatcag gtgaatataa agaacttgta
    27901 aaaagatatc ttttagaata ctacgaccca ctttatatgc attcagtaga aaaatataag
    27961 tatagtaaga ttataaattt taatgataca caaaaagctt tagaagatgt tatagactta
    28021 tatgagagta tattggaagg agaaagtgaa tgttaaaaat agcattagaa tcattaggat
    28081 gctcaaaaaa tttagtagat gcagaaataa tgatgggaat tttaaataac aaaggctata
    28141 aattaattgg agattttgaa gaagctgatg taattatagt aaatacatgt ggatttatag
    28201 agtcagctaa acaagaatct atagatacga taataaactt tgcagagtta aaaaaaactg
    28261 gcaatttaaa gttgttgata gtaacagggt gtctagccca aagatattct gaagaattaa
    28321 aaactgaaat tcctgaaata gatgctatag taggaacagg aagctatcaa aatatagata
    28381 aaatacttaa agaattaagt gaaatgcata aaattgtaag ccttaatgat atagagtttg
    28441 tatttaatga agatttacca agatatatat ctacacctag ctatatggcc tatttaaaaa
    28501 ttggagaagg atgttccaat aattgtactt attgtataat accaaaacta agaggtaaat
    28561 atagaagtag aaaatttgaa gatataatta aagaagctaa gaaattagct gaatctggag
    28621 taaaagaact tgtggttata gcacaggata cgacaaagta cggttttgat ttatatggaa
    28681 aagaaagatt atcagaactt ttagaagaac ttgcaaaaat agatggattt aagtggataa
    28741 gggttatgta ttcatatcca gaatctataa cagaagaatt gatacaagtt ataaagaaat
    28801 atgataatat atgtagttac tttgatatgc caatacagca tgcaagtaac aatattttaa
    28861 aattaatgaa tagaaaaact actaaagaag atatattaaa taaaatcaat ctgataagaa
    28921 gtaatatacc agatgctata cttagaacta caataatagt agggtttcct ggtgaaacag
    28981 aagatgactt caaacaactt gtggactttg ttgaagaggt taaatttgat agacttggtg
    29041 catttgcata ttcaagagaa gaagatacac cagcagatag attgccaaac catatagatg
    29101 aagaagtaaa aatacaaaga agagatactt taatgatgat tcaacaaaaa atatctgaag
    29161 aacttaatga caaaaagata ggaaaaactt atgaggtgtt aatagaagaa cagatagaag
    29221 ataatgtata tacaggaaga acacaaggtg atgcagaaga aattgacagt atagtgtatg
    29281 taaaaagtgt agacaatctt gaagtaggag aatttgtaag tgttcaaata aatgatgcaa
    29341 tggaatacga tttaatggga gatgttttat atgaacttgc ctaacaagtt aactttgttt
    29401 agaatatttt taataccagt atttgtttta attatgttgc tcaatatacc aaataaattt
    29461 ttaatagctt gtattatttt tataatagct tcaattactg atgctttaga tggaaaaata
    29521 gctagaaaat acaatctagt cactgacttt ggaaaattta tggacccatt agcagataag
    29581 ttattggtta tttcagcact tacatgtatg atagaagacc atcttgtaag tagttggatg
    29641 gtaattataa tagtggctag agagcttaca gttagcatat taagagctat agctgctgct
    29701 gatggaaaag taattgctgc tggaaacagt ggaaaactta aaacgataac tcaaatggta
    29761 tctatagtgt ttttattgtt aggtgctcag tttgaaaatg tattgatatt aaatattggt
    29821 gaaattctca ttcttatagc tacactactt acattatatt ctggatggga atatctatat
    29881 aaaaataaag agctttttat gtcttctaag taaaaaatta tgctttaata aataaaaact
    29941 aatgattaat aaataaaaaa taacaactta aaattttaaa aatgtaaaaa agggtatact
    30001 attatagtat acctcttttc attttaagaa tattttcttt tgtgtatata attattaata
    30061 atggtttata aataaaatgt aatgaataaa aatataatca ttagtagcgt ttttaaattg
    30121 actaggtttg atttatgtta tataattaat atataacaag agaacaaatg tttgtagata
    30181 aatgaaggga tggtatagag atgagtgtag atcaagaaaa attaaaagcg ttgaatgaag
    30241 ctttaggtaa aattgaaaaa gattttggta aaggttcagt aatgaaattg ggagaagcaa
    30301 catctatgtc tatagatgtt atatcaacag gagcgattgg tttagacata gctattggta
    30361 taggaggtct acctagaggg agaatagttg aagtatatgg tccagaatct tctggtaaga
    30421 ctactgttgc gcttagttgt gtagcatcag ctcaaaaaga tggaggaata gctgcattta
    30481 tagatgcaga acatgcactt gacccagtat atgcaaaagc tttgggtgtg gatgttgata
    30541 acctaataat atctcaacca gatacaggtg aacaggcttt agagatagca gaggcattga
    30601 taagaagtgg agcgatagat ataatagtaa tagactcagt agcagcatta gttccaaagg
    30661 ctgaaataga tggagatatg ggtgattctc atgtaggttt acaagctaga cttatgtcac
    30721 aagcacttag aaagttaact ggttcaatta aaaaatcaaa ttgtgttgct atatttataa
    30781 accagttaag agagaaagta ggaataatgt ttggaaaccc agaaactact actggaggac
    30841 gtgcactaaa attctattca tcagttagat tggatgttag aaaaatagat acaataaaac
    30901 aaggtgataa agttataggt agtagaacta gagttaaagt tgttaaaaac aaagtagcac
    30961 caccatttaa gcaagctgaa tttgatataa tgtatggaga agggatttca aaaattggag
    31021 accttttaga tatagctgct gatgtagata tagttaaaaa atcaggttca tggtatagtt
    31081 acaatgatac taaacttgga caaggaagag aaaatgttaa aaaattcttg gaagataatt
    31141 tagatttaac tactgaaata gatgagaaag ttagagcatt ttacaattta aatgaagaac
    31201 atgaagaatc aggtacttca gtatcaaaag aaattgtaga agaataagta aaaatacaaa
    31261 aaaataacta gtaattaaag acatgccacg ctccctatta gcttgacaca atgttgaaaa
    31321 aattataaaa ttaagtgagg ataaaatatc taaatttaat tttaattatt aggaatattt
    31381 tttaatcata gtgatgacat tttgggatta ttaacgaagg aggtggatgt catagatagt
    31441 gtagtaatga ttgtaatagg tgcagctgtt ggtattatag ctggatattt tgttagaaaa
    31501 aatatatctg aagcaaaaat tggacaagct gaaaacttgg ccaaagaaat aatagataaa
    31561 gcacaccacg actcagaaac agtacaaaaa gaaaaattac tagaagctaa agaagagatc
    31621 cataagtgga gaacagaagc tgaaagagaa aacagagaaa gaagaacaga agtacaaaag
    31681 tatgaaaaaa gagtagtaca aaaagaagaa gtcttagata gaaaattaca aaatttagaa
    31741 tctaaagaag taaacctaag tgaaaaatta aaggtagtgg aaaagaaaga agaagaagtt
    31801 gaagtaataa aaactcaaca gttagaaaaa ttagaaagta tatcaggaat tacttctgat
    31861 aaggccaaag aaatcatctt aaccaatgcg gagagagatg taagacgtga aatgtctata
    31921 atgataaaag agatagaaag tcaagctaaa gaagaagctg ataagaaatc aagagaaatt
    31981 ataggatatg caattcaaaa atgtgcagca gatcacgtag ccgaaactac tgtaacagta
    32041 gtaagcttgc ctaatgatga aatgaaaggt agaataatag gtagggaagg tagaaatata
    32101 aggactttag aaaccttaac aggtatagac cttataatag atgacactcc agaagccgta
    32161 attttatcag gatttgatcc aataagaaga gaaattgcaa gaattgctct agaaaaatta
    32221 atagcagatg gaagaataca tccagctaga attgaagaaa tggttgaaaa ggcgagaaaa
    32281 gaagttgaca atattataaa agaatatggt gaacaagctg catttgaaac tagtgtacat
    32341 gggattcatc cggaattaat aagattatta ggtagactta actatagaac tagttatgga
    32401 caaaatgtac ttaaacattc tatagaagtt gctcatatag ctggtataat ggctgctgaa
    32461 ataggagcag atattagatt ggctaagagg gcaggtttat tacatgatat aggtaaggct
    32521 gtagaccatg aaatggaagg tacacatgtt gaaataggta tggatttact tagacgttat
    32581 aaagagtcta aagaagttat acatgccatg agtactcatc atggagatta cgagcctcaa
    32641 acagtagagg ctgtattagt tacagcagca gatgcaatat cagctgctag acctggtgcg
    32701 agaagagaaa ctttagaagc atatataaag agacttgaaa aattagaaga aatagctaat
    32761 tcatatgaag gtgtagaaaa atcatttgct atacaagctg gtagagaaat aagaataatg
    32821 gtaaaaccag aagctataaa tgatgaagaa atacatctat tggctagaga tatgactaag
    32881 aaaatagagg atgaacttga atatcctgga caaataaaag taagcataat aagagaaaca
    32941 agagctatag agtatgcgaa gtaaaagaaa taatatctaa ataataagaa aatattttta
    33001 gaaatttaaa tttgccacaa gatatgataa tatatcttgt ggcttttaaa ttgtttaagt
    33061 ttaattttat ataatgcaaa aataatctaa gtgctaaaag tgctacttac aaaagattaa
    33121 taaaataaag ctttatatat gtattattta tataattatc caagtttgtt tgatataatg
    33181 aagttgtaaa ataaatatat tactacttgg aatgcttgta cttaaataag atgaaaatac
    33241 aatgacgcta aagtgaggat tagagagagg gtacactatg aaaaaaaata cacttttaca
    33301 tacaaatatt atggtttgca ttattattac tcttggtttt gtggctacct ctataattgg
    33361 atatcaatct aataccaata tttttaaaaa tgatattgaa catgtatcta cattggctgc
    33421 tgaaggtatt tattatcaga tagataaact actttcagaa cctataaatg tatctcttac
    33481 aatggcaaat gacagtctat taaaaaattt tttagatggt gagaaagaac atttaaatga
    33541 tgaagaattt atttataaat tacaagatta tctgaatgtg tatagaaata aatattcata
    33601 tgattctgtt tttttagtat caactaaaac caataattat tatcatttta atggacttga
    33661 tagaaatctt agcgctaata actcagagaa tcaatggtat tatactttct taaagaatga
    33721 tgatgaatac tcattaaatg ttgataatga tgaagcaagt aacaatagta ttacagtctt
    33781 tgtaaattgt aagataaaag atgataatgg agcaactatg ggaattatag gtgttgggtt
    33841 aaaggttaat tcattacaaa tgttgcttaa aggatataat gacaaatttg atgttgttgc
    33901 acgcttaatt gatgataaag ggtttgtaca attagcagta gataaaaccg gtcatgagaa
    33961 tataaatttt tttgaaaatt ctagtagcga cttgtctaat tcgaaacaat taatattaaa
    34021 caacaaaaag gaacaaaaga gtttttggta ttcatctgaa aaatcaaaaa gttatattgt
    34081 atgtcagtac atacctagtt taaaatggca tcttgttcta gaaaatgatt ttactttaat
    34141 gattaaacag cttcatctgc aattttttaa aggcattgca gttatagttt taattataat
    34201 atttgtactt tttactatta cacatgtgct taaaaaatac aaaaaccaaa taataaagct
    34261 tagtgtttca cagaaattgg aatatcaaaa attgttgagc aaatctactg aaggtcttta
    34321 tgaaaatatt tttgaaattg atattactaa caataaagct ggttgtgaag atgagaatag
    34381 gtattttcat gaatttggat tagggcttaa taccccatat gatgaagcct tacatacaat
    34441 agctaaaaac caaatcaaag aagaatatgc acaaggttat ttagatacat ttttacctga
    34501 aaatgtaatt aaacgttata atagcggaat taataatatc agctatgatt ttttaattaa
    34561 aaaagatgaa gaaagttatc attggattag aattaatgca cgaattttct tttggacatc
    34621 agatgaatct gttcgtatga tatcttatcg taaaaatata gatgaagaaa aaaatagaga
    34681 attactactt ctcgaacgtt cacaacgtga ttctcttaca gggctttaca ataaagctac
    34741 aacagaggaa tttattcgtt catttttaaa aaatgaaagt aatcaagatt tagaacacgt
    34801 ttttctaatt ttcgatattg ataattttaa aaatgtaaat gataaatatg gtcatgtatt
    34861 tggagacttt gttataagtg aatttgcctt agaattaaaa tctcaatttc gtgaggacga
    34921 tattgtggga cgtattggtg gagatgagtt tgtagtatta ataaaagact ttaataataa
    34981 taactatgtg cttatagaca aattagagcg tttttgttct aagattaatc agaaaaaatt
    35041 tgttgagcaa gcagaatttt ctattgcatg tagtattggt gtagcaatgt atcctgaaca
    35101 tggatatgta tactctgaac tatatgagaa agcagaccaa gcactttact atgcaaaaag
    35161 ccatgggaaa aattcttatc atatattttc agaggattct attgatagta tttcatttca
    35221 tatagactct agagatataa aagtattatt agaaatggct actgatggtg ttgtcaaatt
    35281 tgcttgtatg gaaaacttaa aattacttta tttcaaccaa aaaatgcttg aatttactgg
    35341 atattcagct caaactgtta cttctatagg ttttgatatt ttttcgcctg tattacaaga
    35401 cgacttacaa atggtactag atacattaag agaagcaatt tctaaaagag agacttttga
    35461 aatagttttt cgtttttgtc atacagatgg tcatattttt cctgtaagga tgcaaggtaa
    35521 gtttgtaagt gaattatatc aaaatcgtta tcctgttttt tatgcgattt atacagacat
    35581 atcaagtatc aatactttac tagaaaataa atagtaaata ctacagtaaa atctaaaaat
    35641 aggatataat agtattaata taagaagatt ataaataaaa cctgctaatg aaaatagatt
    35701 atttttagca ggtttttaaa ttttttataa taaatcttat ttttggctaa caagatttat
    35761 tttttttaag actttactta ttattttaag cattaattat gaaatttatc attttattat
    35821 ttacagaaag tattttataa caataaatat ctatacttat tgcaaaattt ttttagcagt
    35881 tttgtattta tttgtattta ttttgaattt atttactaaa tttgcctata aaaagtatat
    35941 ataaaaa
//
LOCUS       DAYATN010000024        35945 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.024, whole genome shotgun sequence.
ACCESSION   DAYATN010000024 DAYATN010000000
VERSION     DAYATN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.024"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(359..871)
                     /locus_tag="ACGI8A_001187"
     CDS             complement(359..871)
                     /locus_tag="ACGI8A_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0179190.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            1321..1797
                     /gene="purE"
                     /locus_tag="ACGI8A_001188"
     CDS             1321..1797
                     /gene="purE"
                     /locus_tag="ACGI8A_001188"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFH0179191.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDNNLRG"
     gene            1803..2501
                     /gene="purC"
                     /locus_tag="ACGI8A_001189"
     CDS             1803..2501
                     /gene="purC"
                     /locus_tag="ACGI8A_001189"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFH0179192.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            2513..3880
                     /gene="purF"
                     /locus_tag="ACGI8A_001190"
     CDS             2513..3880
                     /gene="purF"
                     /locus_tag="ACGI8A_001190"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFH0179193.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            3874..4950
                     /gene="purM"
                     /locus_tag="ACGI8A_001191"
     CDS             3874..4950
                     /gene="purM"
                     /locus_tag="ACGI8A_001191"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFH0179194.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMERYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAVAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            4944..5537
                     /gene="purN"
                     /locus_tag="ACGI8A_001192"
     CDS             4944..5537
                     /gene="purN"
                     /locus_tag="ACGI8A_001192"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFH0179195.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            5530..7062
                     /gene="purH"
                     /locus_tag="ACGI8A_001193"
     CDS             5530..7062
                     /gene="purH"
                     /locus_tag="ACGI8A_001193"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFH0179196.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            7073..8323
                     /gene="purD"
                     /locus_tag="ACGI8A_001194"
     CDS             7073..8323
                     /gene="purD"
                     /locus_tag="ACGI8A_001194"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFH0179197.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            8348..12154
                     /locus_tag="ACGI8A_001195"
     CDS             8348..12154
                     /locus_tag="ACGI8A_001195"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFH0179198.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKETSIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            12360..13232
                     /gene="rfbD"
                     /locus_tag="ACGI8A_001196"
     CDS             12360..13232
                     /gene="rfbD"
                     /locus_tag="ACGI8A_001196"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFH0179199.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            13341..14210
                     /gene="rfbA"
                     /locus_tag="ACGI8A_001197"
     CDS             13341..14210
                     /gene="rfbA"
                     /locus_tag="ACGI8A_001197"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFH0179200.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            14259..14816
                     /gene="rfbC"
                     /locus_tag="ACGI8A_001198"
     CDS             14259..14816
                     /gene="rfbC"
                     /locus_tag="ACGI8A_001198"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFH0179201.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            14854..15837
                     /gene="rfbB"
                     /locus_tag="ACGI8A_001199"
     CDS             14854..15837
                     /gene="rfbB"
                     /locus_tag="ACGI8A_001199"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFH0179202.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            15867..16535
                     /locus_tag="ACGI8A_001200"
     CDS             15867..16535
                     /locus_tag="ACGI8A_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFH0179203.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVNQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            16544..16972
                     /locus_tag="ACGI8A_001201"
     CDS             16544..16972
                     /locus_tag="ACGI8A_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HFH0179204.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            16996..17310
                     /locus_tag="ACGI8A_001202"
     CDS             16996..17310
                     /locus_tag="ACGI8A_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFH0179205.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVKVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            17461..17736
                     /gene="flgM"
                     /locus_tag="ACGI8A_001203"
     CDS             17461..17736
                     /gene="flgM"
                     /locus_tag="ACGI8A_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFH0179206.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            17741..18157
                     /locus_tag="ACGI8A_001204"
     CDS             17741..18157
                     /locus_tag="ACGI8A_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HFH0179207.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGNDISMSEVVENSNDGYLKEIYTDLKRILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            18175..19485
                     /gene="flgK"
                     /locus_tag="ACGI8A_001205"
     CDS             18175..19485
                     /gene="flgK"
                     /locus_tag="ACGI8A_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFH0179208.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            19504..20436
                     /gene="flgL"
                     /locus_tag="ACGI8A_001206"
     CDS             19504..20436
                     /gene="flgL"
                     /locus_tag="ACGI8A_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFH0179209.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            20452..20844
                     /gene="fliW"
                     /locus_tag="ACGI8A_001207"
     CDS             20452..20844
                     /gene="fliW"
                     /locus_tag="ACGI8A_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HFH0179210.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            20838..21050
                     /gene="csrA"
                     /locus_tag="ACGI8A_001208"
     CDS             20838..21050
                     /gene="csrA"
                     /locus_tag="ACGI8A_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFH0179211.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            21072..21467
                     /gene="fliS"
                     /locus_tag="ACGI8A_001209"
     CDS             21072..21467
                     /gene="fliS"
                     /locus_tag="ACGI8A_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFH0179212.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            21467..21823
                     /gene="fliS"
                     /locus_tag="ACGI8A_001210"
     CDS             21467..21823
                     /gene="fliS"
                     /locus_tag="ACGI8A_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFH0179213.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            21844..23367
                     /gene="fliD"
                     /locus_tag="ACGI8A_001211"
     CDS             21844..23367
                     /gene="fliD"
                     /locus_tag="ACGI8A_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFH0179214.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSINSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            23370..23705
                     /locus_tag="ACGI8A_001212"
     CDS             23370..23705
                     /locus_tag="ACGI8A_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFH0179215.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            23807..24679
                     /locus_tag="ACGI8A_001213"
     CDS             23807..24679
                     /locus_tag="ACGI8A_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFH0179216.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            24772..26913
                     /locus_tag="ACGI8A_001214"
     CDS             24772..26913
                     /locus_tag="ACGI8A_001214"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0179217.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            26937..28814
                     /locus_tag="ACGI8A_001215"
     CDS             26937..28814
                     /locus_tag="ACGI8A_001215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0179218.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            28807..30921
                     /locus_tag="ACGI8A_001216"
     CDS             28807..30921
                     /locus_tag="ACGI8A_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0179219.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYMESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIKRYNTSEFDRDRIKEKQSEIKHFVIDKHCIEIKNYIDKIYNEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            30930..31925
                     /locus_tag="ACGI8A_001217"
     CDS             30930..31925
                     /locus_tag="ACGI8A_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HFH0179220.1"
                     /translation="MNNIHINKFEKSCERNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLREQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            32017..33249
                     /locus_tag="ACGI8A_001218"
     CDS             32017..33249
                     /locus_tag="ACGI8A_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFH0179221.1"
                     /translation="MKRLMILGSIKYFENIVISAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIELYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            33269..34213
                     /locus_tag="ACGI8A_001219"
     CDS             33269..34213
                     /locus_tag="ACGI8A_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HFH0179222.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ELFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            34286..34714
                     /locus_tag="ACGI8A_001220"
     CDS             34286..34714
                     /locus_tag="ACGI8A_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HFH0179223.1"
                     /translation="MNRIDKFMDKYNLIRFVSIGNEYIGNLVALESNKVIPFDIKRVY
                     YIYDVPNNVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGV
                     WHEMFDFDEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            34792..35892
                     /locus_tag="ACGI8A_001221"
     CDS             34792..35892
                     /locus_tag="ACGI8A_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFH0179224.1"
                     /translation="MNLLLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHF
                     EEEFANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGAT
                     PVFVEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDC
                     AQSHGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGS
                     EKRYYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKI
                     ENNCTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNI
                     AENYSDKVVSIPLFIGMEDEEVSYIIKIINEY"
BASE COUNT        14726 a         3618 c         6545 g        11056 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaata aaatacttta atgaaaaata gctaacttta attgtaggat attcttaata
      181 tatttgaaat aaataaaaat attgaaggat ataatattca actggtatat aaataataaa
      241 tgatagttaa attttacctt agaggctaat taggtgttta gtcaaagcta atgcctttag
      301 aatacaaatg tattctaaag gcatttttac tatgtatttt catttatatt actaaacatt
      361 atctattttt atatacattt ctatgtattt tattttcatc aaatcggtct ataaactcat
      421 ttttttcttc actatatcca atagatataa gtgcataagg aactaatttt aatggtaagt
      481 ctaaagtttg tctaacttta tttaccactt tatttttagg aaatactcct gcccaacaag
      541 cagctagacc ttgattagta acttctaata ataaattttg tgtgcaagca cctaaatctt
      601 gtatccattt accaggtttt aagaatttat ctctatttcc aagcactact atacataatg
      661 gtgcaaattc tatatgcttt gctctatgct gtgactttga gagctgtttc agcagttcct
      721 tatcatctac tataataaat tcccaaggtt gagcattttt tgatgaaggt gcttgcatag
      781 ctgattttag tatttcagtt attttatctt cttctatatt tttatctata tatgttctta
      841 ttgaacgtct attttttata acttctaaca tacagattct cctttaaata aaattatttc
      901 tatttatata atatcattac tttaaatttt agctattact tatataaaga ataaactttg
      961 tgttggtttt atctaagtaa aagtaacatt cagaactaaa aaacaagact ataaatccaa
     1021 agtattaatt aaaaaagaag taataaaaaa taatatacaa gatttaaaaa agagtattgg
     1081 gcttaaaatt taattagaaa accaattttg agataatctg ttttttattt taacaaaaaa
     1141 acataaataa agttcgatac gtagcgaatg aaaaaattac aagaaatatt ttttttaaaa
     1201 aatttcaaaa aagagttgat aatatatgta tattaatata aaatttaagg tgtataattc
     1261 gtataataat acgaatgatt tgtagtgtaa aataacttat aaacggagag gggaagtcta
     1321 atgaaagtag cagtagttat gggttcaaaa tcagattatc caaaattaga agaagggata
     1381 aaactgcttg aaaaatatgg aatagaagtt gtagcaagag cattatcagc tcatagaaca
     1441 ccagaacaac tttcaatatt cctaaaagaa atagaagatg atactgatgt aataatagca
     1501 gcagcaggaa aagcagcaca cttaccaggt gtaatagctt cacaaacatt aattcctgta
     1561 ataggattac ctattaaatc atcaactatg gatggactag actcactttt atcaatagtt
     1621 caaatgccaa aaggaatccc agttgcaaca gttacaatag acttaggttt aaacgcagct
     1681 ttattggcat tacaaataat gactttaaaa tacccaaaat taaaagagga tttgaaatca
     1741 tatagagaag agatggcaca aaaggtgtta gaagacgaca ataatttaag ggggtagcag
     1801 aaatgttatt atatgaagga aaagcaaaac aagtatattc tacagataat gaaaatgaat
     1861 atgttgttta ttataaagat gatgcaacag catttaatgg agaaaaaaaa gcagaaatat
     1921 cttcaaaagg tatactaaat aataagatat caacaataat ctttgaaatg ttaaaagaaa
     1981 ataatataaa tactcacttt ataaaaagtt tatcagatag agaaatgtta gttaaaaaag
     2041 tagagatttt accattagaa gtaatagtaa gaaatattgc tgctggttct atctgtaaaa
     2101 gagttggtct tgaagaaggt gtagttttcg atgagccaat ttttgaaata agctacaaaa
     2161 atgatgctta tggtgaccca atgctaaatg atgattatgc agtggcaatg aagttagcaa
     2221 ctagagaaga gttaaaattc ttaagagaag aaactttaaa aataaatgag ttactaaaag
     2281 catttttctt aaaattaaac ctaaagttag ttgactttaa aatagaattt ggtaaggatt
     2341 ctgaaggaaa tataatattg gcagatgaag tatcaccaga cacttgtaga ctttgggatg
     2401 taaatacaaa tgaaaagtta gataaggaca gatttagaaa agaccttgga gaccttgtag
     2461 aaggttatac ggaagtactt tcaagaatga ataataaata ggaggcaaat gtatgtgtgg
     2521 agttttaggg atttactcta ataaagatgt aactaaagaa ctatattatt ctttatattc
     2581 tatgcaacac agggggcaag aaagctgtgg tttagctctt ttagatgatg gagaaattaa
     2641 gtacaaaaaa gatatgggat tagttggaga tgtttttaag gaaaatgaat tatctaagtt
     2701 aaaaggaaat atagggattg gacatgttag atattcaaca gctggaggaa gtcatgtttc
     2761 taattgtcaa cctttagttg gaagttgtag aaaaagacaa ttagccatag ctcataatgg
     2821 aaatctagtt aatgcaaatt atttaaaaga tatgctagaa gaagatggat atatgttcca
     2881 aactaattca gatacagaag tgattttata tatacttgct agatattaca aaggggatat
     2941 agtagaaagt ttaaaagtga caatggatta tataaaaggt gcatatgctc ttgtaataat
     3001 gagtcaagaa gaattggttg cagttagaga cccacatgga ttcagacctc ttgtacttgg
     3061 taaaaagggt gatgaatata tatttgcatc agaaaattgt gcaatagata tacttggcgg
     3121 agaagttata agagatgtag agccaggaga gataatagta gtcaaagatg gagaactaaa
     3181 atcatatttt tattcagaaa attataaacc agttaagaaa agttgtatat ttgaacacat
     3241 atactttgca agaaatgatg caactataga caatgtaaat gcatatgaat ttagaattaa
     3301 atgtggtgag agattagctc agaatgaaac tgtaaaggca gatatggtag ttccagttcc
     3361 agattcaggt tggccaggag ctattggtta tgcaaatgct agtggattaa aaataagtga
     3421 aggcttagtt aaaaatagat atgtaggaag aacatttata aaacctacac aagaagaaag
     3481 agaaatagca gtaaaaataa aattaaatcc tctttcaaca ataataaaag gaaaatctat
     3541 aattttagta gacgactcaa ttgtaagagg aactacatct aaacaattag taaaatcttt
     3601 aagagaagca ggtgcaaaag aaattcatct tagaataact tctcctccag tagcttactc
     3661 ttgttactat ggaatagata ctccaaatcg ctctaaatta attgcatcaa gtaacaacgt
     3721 agaagaaatg agagaatata ttggatgtga tagtttaaaa ttcctagata tagaaggaat
     3781 gttagatgca acagaacata agtctacttt ctgtaaagct tgttttgatg gagaataccc
     3841 agttaagaaa attgataagg aggaactact ttcatgttaa catataaaga atcaggtgta
     3901 gatatagatg aaggaaatag agcagtagac cttataaaag gtaaaataaa aggaacttat
     3961 gatggaaatg tagttggaga tttaggtaac ttcagtggat tatacagttt aaaagatttt
     4021 gtaggtatga aagaaccagt tttgcttgca tctacagatg gtgttgggac taagttaaaa
     4081 atagctcaga tgatggacaa gcatgatact gttggaatag atttagttgc aatgtgcgtg
     4141 aatgatttaa tttgtcaagg agcaaaacca ttattcttcc ttgactatat agctcttggg
     4201 aaattagtgc ctgagcatat agaaaaaata gttggtggaa ttgcagatgg ttgcaaaatg
     4261 tctggttgtg cactaattgg tggagaaact gctgaaatgc ctggaatgta tggagaagat
     4321 gactatgatt tagcaggatt ttctgtggga atagcagaca aagaaaagat tgtttctgga
     4381 aacaatgtta aatcaggaga tgtattggtt ggtatatctt caagtggagt acacagcaat
     4441 ggattttctt ttataagaaa gatattttta gaaacttaca attataaaat ggaacgatat
     4501 gttgaagaac taggaatgac agttggagaa gcacttttaa caccaactaa aatatatgtt
     4561 aaattagctt tagatgtatt agctaaacat gacataaaag ctgtagctca cataactggt
     4621 ggagggctta tagaaaatat aacaagagtt atacctaaag gattaggact agatatcaat
     4681 aaaaaatctt gggagaagcc tcctatattt aaaatgatag aaggttttaa tgctgttgat
     4741 gagagagaac ttcataagag ttttaacatg ggtattgggc tagttttaat agtagataaa
     4801 gaaaatgctg atgatgttgt aaactttata aataatagag aaaatgacaa tgctgattat
     4861 gtagataaaa aatatagtga attattagaa gataaagctt atataattgg tgaagtagta
     4921 gatagtcatg aaggtgtaga attatgctaa atataggagt tttaatatct ggtgggggaa
     4981 caaatttaca agcagttata gatggtactg agtctggaga aataaaaggt caggttaaag
     5041 tagttatctc aagtaagcaa ggtgcttatg ggctagagag agctaaaaat cataacataa
     5101 aagcaatttg tgaaactgat gaagacaaga taatagaaat tcttaaggaa aataaaatag
     5161 atttagtagt tttggctggt tatttaaaaa taatcagccc aaagctggtt aatgagttta
     5221 gaaacaaaat gataaacata catccatcat taattccttc attttgtgga gctggattct
     5281 atggagaaaa agttcatcaa ggtgttatag attacggagc taaagtaact ggtgctacag
     5341 ttcattttgt tgatgaaggt gctgatactg gaccaatcat aatgcaagat gtagttaagg
     5401 taaatcaaga tgatgatgca aagacacttg caaaaagagt gttagaagtt gaacatagga
     5461 ttttaaaaga aagtataagt cttttttgtg aaaataaatt aaaacttcaa ggtaggagag
     5521 tgtttataaa tgagtaagag agctttaata agtgtaactg acaagactgg tgtagtggaa
     5581 tttgctaaag agttaaataa attagattat gaaattatat ctacaggaaa tacatttaaa
     5641 acattaaaag aaaatggtgt taatgtaatg caagttgaag atgttacaaa ttttccagaa
     5701 atattagatg gaagagtaaa aacattaaat ccatatatac atggtggtat actttataaa
     5761 agagataaag aatctcatgt agaaactgta aatgaacata aaatacattc aatagattta
     5821 gtagctgtaa atctatatga ttttgaagga actttaaaag ctggaaaaag tcatgatgaa
     5881 ataattgaaa atatagatat tggtggacca tctatgatac gttctgctgc caaaaactat
     5941 aaggatgtaa tagtagtagt agatataaaa gattatgatt ctataataga aaaattaaaa
     6001 acagatacaa tgacattaga agacagaaaa aaattatcat ataaagcttt ctcaactaca
     6061 ggaagatatg atgcgttaat atctagttat tttgctggag aagttggaga tacgtatcct
     6121 gacatattaa atttaacttt ccaaaaagaa caaactttaa gatatggaga aaatccacat
     6181 caaaatggtt tcttatattc acagtcaaat gcaaaaaatc caatcttaaa ttatgagcaa
     6241 ttaggcggta aagaactttc ttttaacaac ttaaatgatt tacatggatg tttagaagta
     6301 atgagagaat ttaaagatag tgaagaagta gtttcagtag ctataaaaca tgctaattca
     6361 tgtggggtag gtttaggaaa agatgctttt gaagcatata caaaatgtta tgaagcggat
     6421 aaggtttcaa tatttggagg aatagtagga ataacttcta ctatagataa agctacagca
     6481 gagaaattaa atgaaatatt cttagaaata gtagtagcat atgattttga accagaagct
     6541 ttagagatac ttaaacaaaa gaaaaatcta agaatattaa aattagctaa aatagaaaat
     6601 agtttacaac catatgaaat gaaatattta gatggaaaat tattgataca agatagaaac
     6661 aatatattag cagaaaaaag tgaaaatgta acaaaggaaa aaccaacaga tgcacaactt
     6721 aaggacatgg aatttggtat gagagtagta aaaaatatga aatcaaatgc aattgcaata
     6781 gtaaaaaatg gacaaactct agctttaggt tgtggacaaa catcaagaat ttgggcatta
     6841 aaaaatgctc ttgaaaataa caaagataaa gattttacag gagctgtttt agcgtcagac
     6901 gcatttttcc catttgatga ttgtgtaaca ttagctcatg aatatggaat atcagcagta
     6961 gttcaaccag gtggctctat aaaagataaa gattcaatag aagcttgtga caagtatgat
     7021 atggttatgg tatttacagg aataagacac ttcaaacact aaggaggata ttatgaagat
     7081 attggttgta ggcggtggcg gaagagagca tgctatatgc tggaaactaa gcaaagaaaa
     7141 aaatgtagaa aaaatttatt gtgcaccagg taatgcagga attgcaaacg tagcagaatg
     7201 tgtaaatata ggtgatacta atatagaaga acttttaaaa tttgccaaag aaaatgaaat
     7261 aggtctgaca atagtaggac cagaagtacc actagttatg ggaatagtag atgagtttga
     7321 aaaagaagga ttaagagtat ttggacctaa taaaaaatgt gctcaacttg aaggaagtaa
     7381 agctttttca aaagaattta tgataaaaca taacatacct actgcaaaat ataaagaata
     7441 tacaaactta gaagaagcta tatcagaaat agatagtttt ggatatccag tagtaataaa
     7501 agcggacgga ttagctgctg gaaaaggtgt tgtaatacct gaaaatagag aagatgctat
     7561 agcaacttta aaagaaatga tgtcagataa gaagtttgga gctgctggag ataaaatagt
     7621 aattgaagag tttttaaaag gaatagaaac ttcaattcta gcatttgtag ataatgatac
     7681 aatagttcct atggcaagtg caaaagacca taaaaaagta aacaactatg agcaaggtcc
     7741 aaacactggg ggaatgggaa ctttttcacc tagtgaaata tatacagaag aattagcaaa
     7801 taaagtaaaa gagacagtat tagaaaaaac tttagaaggc ttcaaaaaag atggacttaa
     7861 ttttaaaggt attttatttg ttggtcttat gataactgaa gatggagaaa aagtacttga
     7921 atataatgta agatttggag accctgagac acaatctgtt ttatttagat tagaaacaga
     7981 tttacatgag ataatggaag ctatactaga taataagtta aaagatatag aaataaacta
     8041 tagtgatgat gaagctgtat gtgttatgct tacatcagga gggtatccag acagttatga
     8101 aaaagggaaa attataactg gtcttgaaaa cttggatgat gatatagtag tattccatag
     8161 tggcactaag atgtttgatg gtaatttggt taccaatgga ggaagagtaa ttggtataac
     8221 tgcaaagtca actacagtta aggatgcagc agaaaaagtt tatgaaaaca taaagaagat
     8281 aaactttgaa ggaatgcact atagaacaga cataggaaga taatttttct aaaatggagg
     8341 ataaggtatg ttgaatactg aaaataaaga ctcaatggtt agaagagtat tagttgaaaa
     8401 aagagaaggt tttgaccttg aggctaaagc tttaaaaaaa gacttagtag aaagtttaca
     8461 catagataat atagaaaatt taagaatatt aaatagatat gatgtagaag gaatatcaga
     8521 agaagtttat gaaaatgcag caaagacaat attctctgaa ccaaatttag atgtagtata
     8581 ttatgaagaa ataccaaaat taaatgatga aagagtattt gcaatagaat ttcttcctgg
     8641 tcaatacgac caaagaggtg attgggcagc tcaatgtgta caaattgtaa atcaagggat
     8701 aagacctgct attaatacag caaaagttta tatattatca ggcaaaataa ctgatgaaga
     8761 gttttctaaa ataaaagatt actgtataaa tccagtagat agtagagaag catctttaga
     8821 aaaaccagaa acgttaaaaa tggaaactga aataccaaca acagttgaag tattagatgg
     8881 atttatagat ttagatgaaa atggattaaa aacatttgta agtgaaaaag gattagcaat
     8941 gactttagga gacttacaac acgttcaaaa atactttaaa gatacagaaa agagaaatcc
     9001 aactataaca gaaataaaag tattagatac ttattggtct gatcattgta gacatactac
     9061 ttttatgact gaaatagaaa atgtaaagat agaagatggt aagtttaatg atatagtaaa
     9121 agaagcgtat caaatgtatc ttaactcaag agacaatgta tatgtgaaca gacataaaga
     9181 tatatgttta atggatatag ccactgttgc agtaaaagaa ttaaagaaaa atgggaaatt
     9241 aaatgaccta gatgaaagtg aagaaataaa tgcatgtagt ataaacgtag atgttgaagt
     9301 tgatggtaag atggaaaaat atctagtaat gtttaaaaat gaaactcaca accatccaac
     9361 tgaaatagaa ccatttggtg gagcagctac ttgtttagga ggagctataa gagacccatt
     9421 atcaggaaga agttatgttt atcaagcaat gcgtgttaca ggaagtgctg acccaagaac
     9481 gactttagaa gatactttac ctggaaaact tatgcaaaga aaaataacta cagaagctgc
     9541 tcatggatat agttcatatg gtaaccaaat aggtcttaca actggacaag ttgcagaagt
     9601 atatgatgaa aactttgttg caaagagaat ggaaataggt gcagttatag cagcagcacc
     9661 aaaagagaat gtagtaagag aaagacctga agcaggagat gtaatcgttc ttcttggagg
     9721 aaaaacagga agagatggct gtggaggagc aactggttca tctaaagaac atagtgaaga
     9781 gtcaatctta acttgtagtg cagaagttca aaaaggagat gctcctaacg aaagaaaaat
     9841 acaaagattc tttagaaata aagaagttgc tcaaatgata aaaagatgta atgattttgg
     9901 agcaggtgga gtttgtgttg ctataggaga gattgctgat agcttagata taaacttaga
     9961 cttagttcct aaaaaatatg atggacttga tggaactgag cttgcaattt cagagtcaca
    10021 agagcgtatg gcagtagcca taaaaaaaga aaataaagat aaatttatac aattagcagt
    10081 agaagaaaac ttagaagcta cgcatgtagc tacagtaaca gatacaggat atttaagaat
    10141 gttctggaat ggaaaagcaa ttgtagatat aaatagagag tttttagata caaacggagt
    10201 aaaacaaact actgatgttc atgttactaa agtagatgaa gaaaatacat tctttagctc
    10261 taatgaaata gtaaaagatg ttaagtgtgc aagtatgaaa gacaagttta caaaagtact
    10321 atctgacctt aatgtttgtt ctcaaaaagg attagtagag atgtttgata atacaattgg
    10381 tggtaataca gtacttatgc catttggagg aaagtatcaa gcgactccaa cacaaggtat
    10441 ggttgctaag ataccagtac ttggaggaga aactaatact tctacaataa tgacttatgg
    10501 atacaatcca aaggtaggaa aatggagccc attccatggt gccttgtatg cagtagtaga
    10561 atctgtgtgt aaattggttg ctattggagg aaattatagt acaacaagac ttacattcca
    10621 agagtatttt gaaaaacttg gtaacaatcc agaaaaatgg ggtaaaccat tctcagcact
    10681 tttaggagca ttttatgcac aaagtaaatt tgagatacct gctataggtg gaaaagacag
    10741 tatgtctggt acatttaaag atattgaagt tccaccaaca ttagtttcat ttgctgttga
    10801 cacagtagat gcgaagaaag tagtatctcc agaatttaaa aaagcagact caaaagtagt
    10861 aatgttatgt gtaaacaagg cagaaaatga tgttgtagat tttgaggaat taaagagaaa
    10921 cttagataaa gtaagagaac ttatacatgg aaataaagtt ttatcaacat atgcattagg
    10981 atttgcaggt gtaggtgaag caataagtaa aatggctttt ggtaataaaa taggatttaa
    11041 gtttagtgaa gaggctgaaa aagcatttac agatgataaa ttatttgagg ctagttatgg
    11101 aaatatagta cttgaattag caaatgatga tttaagtatg ttagaaggat acaattatgt
    11161 tgtgcttgga agtacagtta aagaaactag tatatttatc aaaggtgaag aacttgcatt
    11221 agatgaatta tataaagcac attgtagtac tttagagcct atattcccaa ctaaaacaga
    11281 agaagtaaaa tcaaagatag aaactataag ttatatatca caaggagaag ctaaaaaatc
    11341 atcattaagt atagctactc caagagtatt tataccagca ttccctggaa caaactgtga
    11401 atatgactct gcaagagcat ttgaaagagc aggagctaat gcaagtataa gagtatttaa
    11461 gaatttaact tataaagaca tagaagattc tatagataca atagtaaacg aaataaaatc
    11521 ttctcaaata ataatgttac caggtggatt tagtgctggt gatgagccag atggttctgg
    11581 taaatttata gctactgtat ttagaaatcc tagagtgcaa gaagctataa atgaattttt
    11641 aactcaaaaa gatggactaa tgttaggtat ctgtaatgga ttccaagtat taattaaatt
    11701 agggttagtt ccatatggag aaataagagt tccaagtgaa agtgctccaa cacttactta
    11761 caataatatt ggtcgccatc aagctaagat tgctagaact agaatttcat ctaataaatc
    11821 tccttggtta gctcaaacta atgttggaga tattcataat atagcaattt ctcatggaga
    11881 aggtaagttt gttgccagtg aagatgttat gagagaatta attgctaatg gacaagtagc
    11941 aactcaatat gttgacttta ataatgaagc tacttatgat attgagttta atccaaatgg
    12001 ttcattctat gctgttgaag gtataactag tgctgatggt agagtgtttg gtaaaatggg
    12061 acattctgaa agaattggtg aggaagtata taagaatatt ataggtgaaa aagaccaaaa
    12121 gatatttgaa tctggagtta aatattttag ataaagcttt gatgtttaat tgatagctag
    12181 aatatataat ttagatattt agatttaaag agatgtaata atatgatgat aatcatttaa
    12241 tattacatct cttttttatt tatctaagaa ataataagtt caacctaagt taaaatacaa
    12301 gctgtgttat aattaattat taagtaaaat tgaatattta ttggaggaca acataattaa
    12361 tgaaaatatt aataacaggg tctaatggtc aattaggaaa agaattggtt aaccaattag
    12421 aagcaatcaa tcaaagtata aaccaaccaa aatatgttat attagctaca acaagaagtg
    12481 atttggacat atccaatcag actaatgtag ataattttat tttgcataat aaacctgatg
    12541 tagttgtaaa ctgtgcagca tatacaaagg ttgatgcatg tgaagataat atcgaaattg
    12601 catacaaaat aaatgcttta ggagtgagaa atctagcaat agcatcagaa aaagttaatg
    12661 ctaagcttat tcacatatca actgactatg tattcaatgg tttttctaaa tatccatata
    12721 gggaagacaa taaaactgaa cccaattctg tatatggaaa aagtaaactt atgggagaaa
    12781 aatttgttga acaattctca cataagtact ttatattgag aacagcatgg ttatatggta
    12841 atgggaataa ttttgtaaag accatgataa aactttcttt agaaaataaa gaggttaatg
    12901 tggttaatga ccagtttgga actccaacaa gtacagtaga cttagcaaaa attataataa
    12961 aaatcatgga aacagaacat tatggagttt atcatgctac atgtgaaggt gagtgtagtt
    13021 ggtatgattt tgccaaaaag atatttaaac taaaaaatat agatataaaa gtaaatccaa
    13081 ttaagagtaa cgaatttaaa tgtaaggctc caagaccact atattctgta ttggataatt
    13141 ttatgttgaa gttaattggt ttaaattctt ttagaaagtg ggaagaatcc attgaagaat
    13201 atttagaaag atatgatata aatgaaaagt aaatgtatta ttttgagaaa agaaaataag
    13261 atattataaa aaatataaat atttctttta tttatccgat aatcataata tgacaaaaac
    13321 aaatttaagg atggaaaaag atgaaaggaa ttattttagc aggagggtct ggtacaagac
    13381 tatatcctat aacaaaatgt atatcaaaac aaacacttcc aatatacgat aaaccaatga
    13441 tatattaccc tatgtcggtt cttatgttgg cagggataca agatatatta ataatatcta
    13501 caccaagaga tataaaagca tttgaagaat tatttggaga tggaagttca ctagggctta
    13561 acataacata tgcaattcag gagaaagcaa atggaattgc agaagcgttt ataataggag
    13621 aagaatttat aggaaatgac agagtgtctc ttgtattagg ggataatata ttttatggat
    13681 atggatttag tgaaaggttg gagaatgcag ttaaaagaaa aggagcaact atatttgggt
    13741 atcatgtaag taatccaaat tcgtttggag ttgtagaatt tgataaggat tttaatgtat
    13801 tgtcaataga agaaaaacct acagttccta aatctaatta tgctatacca ggattatatt
    13861 tttatgataa tgatgttgta gagatagcaa agctagttga accgtcagat agaggtgaat
    13921 tagaaataac ctctataaac agtgaatatc taaaacgagg aaaactaaaa gtggaacttc
    13981 taggaagagg tatggcttgg cttgatactg gtacacataa aggtctattg gatgcttcta
    14041 attatgttga agtggttcaa acaagacagg gactgtatat agcatgttta gaagagattg
    14101 catatacaaa aggatatata agtagagaac aattaatgaa actagccaag ccactgataa
    14161 aaactgaata tggtcaatat ctattaaaat tggctagtga agatatgtaa tatggtagat
    14221 aaaatttatt ataaatttta ttaagggagt ttattataat gggaaaaatc aatgttataa
    14281 agactaaaat agaaggatta tatatagtcg aaccaaatgt attttatgat gaaagaggat
    14341 attttatgga aagctataat aaaaaggatt ttatagagca gggtcttgat atgtgtttta
    14401 ttcaagataa tgagtctagc tcaagaaaag gcgttttaag aggaatgcat tttcaggtta
    14461 aatatcctca aggaaaatta gtcagagtta cagagggaga agttttcgat gttgcagttg
    14521 atttaaggaa agattcacaa acatttggga aatgggaagg tgtaatattg tctgataaga
    14581 ataaaaaaca attttatatt ccagaagggt ttgcacatgg atttttagtt ttatcagaaa
    14641 aagcagtatt tagttacaaa tgtacaaatt attattaccc agaatttgag aatggcttta
    14701 tatggaatga caagcaaata tctattgagt ggcctttaaa aggaattgaa gaaatatttt
    14761 tatcagaaaa ggacaaaaaa caaaaaagtt ttaaagatat ttgtacaaga cgttaataaa
    14821 aatttattag aattaacatt taggagaaaa aatatgaaaa aaatactgat aactggagga
    14881 gcaggattta ttgggagtaa ttttatacat tatatgctta acaagtatgt agattatttt
    14941 attgttaacc tagacttgtt gacttatgct ggtaatttag agacattaat agaaatacaa
    15001 gacaattcca attataaatt tgttaaagga gatatttcaa atagggagca ggtgtataag
    15061 ctatttgaag aagaaaggtt tgatatagtt gttaactttg ctgctgaatc tcatgtagat
    15121 aggtcagttg agaatcctga tttatttatt aaaacaaaca ttataggaac acaagtactt
    15181 ttagatgcat ctataaaata tggagttaaa aggtatcatc aaatatctac agacgaagtg
    15241 tatggtgatt taccaattga tagaaaggat ttattcttta cagaacaatc ttctatcaat
    15301 ccaagttctc catattcagc ttctaaagca tcagcagatt tattggtaag ttcttattat
    15361 agaacatatg gattgttaac aactatatct aggtgttcca acaattatgg gccatatcat
    15421 tttcctgaaa aattgattcc tcttatgatt ttaaatgctt tggaaaacaa acaattgcca
    15481 gtatatggga atggggaaaa tgtaagagat tggttacatg tatatgacca ttgtactgct
    15541 atagacttaa taatacataa aggaaatata ggggagattt acaatatagg aggtcataat
    15601 gaaagaagta atctagaagt agtaaaaatg atattaaatc ttttaggtaa atctgaagag
    15661 ttaatatcct atgtaaatga tagacctggt catgatttaa gatatgctat tgatgcaact
    15721 aaaatagaga atgagttagg ttggaaggca aaatatgact ttgatttggg tataaaagaa
    15781 actgttaagt ggtatataga aaatgaatct tggtggaaag ctgttttatc agtataactt
    15841 taaaatttag aaaggggcgt tttatattga atgagataag ttcactaatt aatatattag
    15901 cattgactca attatctggt agtaatacta caaatcaatg taattgtaca tataataatt
    15961 catctggatt tgatatgatt atgatgacct tgttaaaagc attatcacaa aataatcaac
    16021 atacttctaa tagctacaat ttattgtcta attctgagaa tttatttaat gaacttgata
    16081 atgtagtaaa taataccact agtagattta tagatgttga tacaaaagat aaaaatgtaa
    16141 agttgagaat tgaaaatgct gtaaatcaag catcaaaaaa atataacgta gattcaaatc
    16201 ttataaaagc aataattaaa gttgaatcag attttaatcc aaataccgtt tcttctgctg
    16261 gtgctaaggg tcttatgcaa cttatgccag aaaactgcag agacttaggg gttacagacc
    16321 cttttaatat agaacagaat atagatgctg gaactagaca tattaaggaa tacattgata
    16381 tgtttggagg gagcatagaa atgggattga tggcatataa tggtggtcct ggaagaatga
    16441 aaagtagagg agtagaatca atctctgacc tgtataaaat gccaaaagaa acacaaaatt
    16501 atattcctaa ggttatgaaa tattatagag ggtaggcttt attatgggaa taaatataga
    16561 taagctaaac ataagtccaa gtataaatat ggaggttaaa aaactaaaag agagtaataa
    16621 attttctgac aaattaaact ctttaaaaca atcttcttca ttggaagata taaaacgaaa
    16681 tttggaaagt gtaaaaaagg caggagaaag gcttgtactt actcaaaact atggcgatat
    16741 tagtatatat aaaaatgcag taaaagaata cctaaaatct gttgtagata atatgtattc
    16801 acttgataaa aatagtagct tttgggagca tcagtattat aagaatgtaa agattgttga
    16861 tagtaaactt gaggatatga ctagtaagtt aattagtgaa gaaaaagaaa acatagatat
    16921 tgtttctact gttgacatga tacaaggctt attgatagat atgtatagat aaggttatac
    16981 aaggagaaaa attttatgaa tagcaaaaaa gaggatatat atgaattgaa ttttgggcaa
    17041 ttaatagaag aaaaatctgg tgttatagaa gaaaatcaaa atgtttttaa tgcaaaagta
    17101 aaggtagctg tttctatagg aaatacaaca gaaacaataa aaaatatatt agatttaaaa
    17161 gaaggctcta taataaaatt aagtaagaat gtggatgaaa aattagatat atatgcaaac
    17221 gatagattgt ttgcttatgg agaaagcatt attacaaatg agaagttatc tgtaagatta
    17281 tctaaaatag aagattcaga tgtaaattag cattttaaat atatcccttt agtctattct
    17341 gtatgagttt agctaagggg atatttttat aaaaaattta aaatatgtgt gaattatata
    17401 aatattttta ttttatgagc gataactaaa ttataagtat gtaattttgg aggtgaaact
    17461 atggatatta aaaatgtaag ttcaaatatt gtagtgaata attatttaaa gaatacatct
    17521 ggtataaaaa aagtggaaga ggttaaaaaa attaataaaa cagacaagat agagttatct
    17581 aaatttgcaa aggaaatggc aagtgtgtca aatatgagtg atgagaatat tagaaataaa
    17641 aaagtcgatg aaataaaaaa tgcattagaa aatggaactt acaaaataaa accaaaagaa
    17701 attgcgaaaa aaatgataga ggatatgcga ggataaagtc atgattacag acttaaaagt
    17761 tataatatat gaagaaaaaa atataattag taagatgatg gaattattaa aagaacaatt
    17821 tgattatata gttgaaaaag acttagaaaa tttaaatatg ttaaatccta aactagaaga
    17881 tattagcagg gagcttgcat ccatagaaat aaagagaaga caactttttg gtaatgatat
    17941 ttcaatgagt gaagttgttg aaaatagtaa tgatggatat ttaaaagaaa tatatacaga
    18001 cttgaaacgc attcttgctt tagctaaaaa tcaacaagaa tcaaatgatt ctctaataaa
    18061 gaaagaactt atttttacga aaaaaatgat taatttcata aaaccagctg ataagcaaac
    18121 tacaacttat aattcttatg gaaatattag aaaataaata ttaggaggag aaatatggca
    18181 ggattatttt ccattttaaa tacagctaat tcaggaatga atgcgcacca aaaagcaata
    18241 cagacaattt ctcataatat aagtaatcta gatacagacg gttattctag gcaaaggacc
    18301 gagtttgcta caaatagtcc aatgtatatg cctagtttga gtaattcaat tgcaagagga
    18361 caattaggga ctggagtaca tgtaacggat gtaacaagag ctagaaatag tttttatgat
    18421 taccaattta gggcagaagc tcataagtat ggtaagacag tttcaaaata tgattattac
    18481 aatactatag aaactatact aaacgaacca tcagactatg gaatatctgc aggtatagat
    18541 gattttttta aaggatggga gtcattatca aaggatataa atagtgacag taaaaaaagt
    18601 ctagtggtag aaaacgcatc taatttagca actttaataa gtgaaagtta taagaagctt
    18661 gataagttaa aagaagatat agattctaat atagacactg agataaaaca aataaatgac
    18721 atgttaaaaa gtcttgaaga tttaaataaa tctattgata ttatatctgg ttctggctct
    18781 acaccaaatg atttgcttga tgaaagagat agaattttag ataacttgag ttttaaacta
    18841 gactttgaaa atagtgatgt taaaaatatg cttgcagatg gtaaattaga acttaatgaa
    18901 ttaaaaaatc cagatggaac ttggaaaaca ggtatatcag ggaccttaca aggcttgttt
    18961 gaaatgcatg gtaagattga tacatataaa agcgatttaa aagatgtatc tgatggatta
    19021 gctaaacaga taaatgatgt ttataatacg tctgcaggaa taacagtaac agattttttt
    19081 ataacttcaa atgtagatgg tgaagatatt ataaagataa atccagctat aaagagtaac
    19141 ccaaatgaat taaagcttac tgctgatgaa gcttcaaaaa ttgctaagtt aaaggatgaa
    19201 aaaatagata ttggggtggc tggtggaaaa gtaagtacaa taagtgacca ctataaagcc
    19261 tttgcagaat caatcggttt agattctcaa aaagtaaatc aagatgaagc aaatcaacgt
    19321 aaaattataa ataatattga taactcaaga atgagtgttt caggtgtatc tttggatgaa
    19381 gaaatgacag agcttataaa aatacaacgt tcatatcaag ctagtgcaaa agttatgtca
    19441 acagctgtgc aactactgga tgttgtaata aatggaataa tttaatagaa gggggaattt
    19501 ataatgagag tcagtacagg tatgatgtct agcagctatc taaactcact acaagataat
    19561 ctacaaagat tggataaggt taatagacaa gttaatacaa caaaagaaat aaacaagcta
    19621 tcagataatc catataaagc aataaagata ttaaactcaa aaagtgaaat aaagactatg
    19681 gagacttatg ttgagaattg taaagataca gggaattggt tagaaactac tgatactgca
    19741 ttagatcagc ttggaaaatt attatcagac ataaagaaaa ctttggtggc aagtggtgat
    19801 ggtgcatact cagatgatga attaaagact cttagtaatg aaactaatga aaaaatgaaa
    19861 gaaattgcaa atgctttaaa tgcaacacat gaagggaagt acatattttc aggttcaaat
    19921 acgggggtac cacctgttga atgtatagat aatgctgatg gttcagtgag tttaaagttt
    19981 aattctagtc taaatcttaa taaattaaat gactctttga gtatggatat agctcaagga
    20041 ataagtgtag attataacgt taaattgagt agccttggat ttgacccaac aaaaactgac
    20101 ccatttgaag agttaaataa catatcaaaa aaattagcaa atccagatgc tgatgctata
    20161 aaagagctta caacaacttg tttgggcgat atggaacaac taatagaaaa tacagtaaat
    20221 gttaggtcta tatatgggac aaaagcaaat acagtagagg ctatgaagga aaaaaacgat
    20281 gatagtttga tacagttaaa ggatattttg tcacaaaatg aagaaataga ttatggtgaa
    20341 aaacttgttc aattaaaggc agcagagtta acttatcaag cttcactaca aacaggagga
    20401 aagttattta atgtatctat attagattac atataataaa ataacgcaga tatgatgaag
    20461 gttacattaa aaaaaggtat attaggtttt gaaaatttaa aagaatatga gcttttagat
    20521 atagaagata atgattattt aaaagaattt aattctactg aagaagaagg tataggattt
    20581 gttgttgtat ctccatttga gattatcaat gaatatgaaa tagttttaaa tcaggaaaca
    20641 atagaaaaac tagaagtgaa atctccaaag gatgttgtat tatttaatat aattactctt
    20701 ggtcagacat ttgaagaatc aactgtgaac atgaaagctc ctatagtgat aaatataaaa
    20761 actaattttg gaatgcaaat aattttacag gatgataaat attcaataag gcatccatta
    20821 ttgagaggtg ataatggatg ctagtaattt caagaaaaaa agatgaagca gtacttatag
    20881 gagaaagcat agaagtaaaa attgtaggag tagatggaaa caatgtaaaa ctagctatat
    20941 cagcgcctaa caatataagt atattaagaa aagaaatata cgaaaaagta aagagtgaga
    21001 atataaaagc tacaaataaa aatataaaaa ttttaaagtc attaaaataa taagcttgga
    21061 ggaagtaata tatgtatgga gaaaatcctt ataattctta taaacaaaat gctgttttta
    21121 tggcatcaaa ggaacaattg ttacttatgc ttgttgatgg agcagtaaaa tataccaaaa
    21181 tagccagagc agctatctta gataaaaata cacagaaagc gcatagagaa cttgtaagag
    21241 ttcaggatat atttacagaa ctaatggtta cattagacca aaatgcagga caatgggcaa
    21301 aggatatgta caaagtttat gattttgtaa gatatgaact ttcaagagcc aatattagaa
    21361 aagacatcca ggtaatagat aatgtgttac ctgtaataga acaaattaaa gatacatggc
    21421 atgaagctga taaaaaaagt aaagaagaaa gaagtaggta caaataatgg aattaaattt
    21481 agctgaatta gataagttat caaaagagga attactattg atgcttgtag atggcacagt
    21541 aaaatatacc aatatatcca aagaagctct tttaaataac gactatttga aagcacataa
    21601 tgaacttgta agagttcaaa atatatttac agaactaatg actacattag accaaaatgc
    21661 aggacaatgg gcaaaggata tgtataaagt ttatgaattt ataaaatctg aattagcaat
    21721 agctgatgaa aataaagata ttaaaataat tgatgatata ttgccaatag taaaacaaat
    21781 tagagataca tggcatgaag tttacaatca actaaaaata taattagatg gagggataaa
    21841 ggtatgtcaa gtataagtcc tataagagtt acaggtcttt caggaaattt tgatatggaa
    21901 ggcataatcg aagctagtat gattagagac aaggaaaaag ttgataaagc aaaacaagaa
    21961 caacaaatcg ttaaatggaa gcaagaaata tatagaaatg ttatacaaga atcaaaagat
    22021 ctttatgata aatatctaag cataaattct cctaatagta tagtaagtga aaaagcatac
    22081 tcttctacaa gaataaccag ttctgatgaa agtattatag tagcaaaagg ttcagctggt
    22141 gcagaaaaaa taaattatca atttgcagtt tctcaaatgg ctgaaccagc aaaatttact
    22201 attaaattaa attcaagtga acctattgtt cgacagttcc ctccaaatgc cagtggagcg
    22261 agttctttaa ctataggaga tgtaaatata ccaatatctg aacaagatac tacaagtact
    22321 attgtaagta agataaactc cctttgcgca gataatgata taaaggcttc ttatagtgag
    22381 atgacaggtg aattgattat ttcaagaaaa cagactggtt cgtcatcaga cattaattta
    22441 agagtaattg gaaatgacaa tttagctcag caaattgcta atgataatgg tatcacattt
    22501 ataaatgatg ctggtggaaa caaagtggca aatgtatatg gaaagaatct agaagctgat
    22561 gtaactgatg aacatggaag agtaactcat ataagtaaag aacaaaattc atttaatata
    22621 gataatattg actataatgt aaattcaaaa ggaactgcaa agttgacttc tgtcactgat
    22681 actgaagaag ctgttaaaaa tatgcaagca tttgtggatg attataataa actgatggac
    22741 aaggtctatg gtttagttac tactaaaaaa ccaaaagatt atccgcctct tacagatgcc
    22801 caaaaagaag atatgacaac tgaagaaata gaaaaatggg aaaagaaagc taaagaaggt
    22861 atacttagaa atgatgatga gttaagaggt tttgttgaag atattcaatc tgcatttttt
    22921 ggagatggaa aaaatattat tgcattaaga aaactaggta tcaatgaaag tgaaaattac
    22981 aataaaaaag gtcaaatatc atttaatgca gatacttttt caaaggctct tatagatgat
    23041 agtgataagg tatacaaaac actagcaggt tattcttcga attatgatga taagggaatg
    23101 tttgaaaagc taaaagatat tgtatatgaa tattctggaa gttcaacttc taaacttcct
    23161 aaaaaagcag gtatagaaaa aactgcttct gctagtgaaa atgtatattc aaaacaaatt
    23221 gcagagcaag aaagaaatat aagcaggtta gttgaaaaaa tgaatgataa agagaaaaga
    23281 ctttatgcta aatattcagc cttagaatct ttgttgaatc agtattcttc ccaaatgaat
    23341 tatttctcac aagcacaggg taattaatta tggataaatt aaaagaaaaa ttaaatttat
    23401 ataaagatat atctttacaa attataaatt tgattgaaaa ggaagaatat ataaagataa
    23461 gtattaagtt aggtgaaaga caagagatta taaatagtgt aagtgaaatt gatagaaatg
    23521 attttataca gctttataat agaatggaat taattgaaat tgatagcagg ataagagata
    23581 ttttacaagg acaattatta gaagtaaaaa aagaattaca tgagtataag cttactaaac
    23641 aagtgaacac aatgtattat aacttaaata gagaaaaagt aaatattttt aataaaaaag
    23701 tataaagtta tgtaccatct tgtccgatta tataaatgaa gaattaataa aaaagattaa
    23761 aagtagaaat gacaaggatg tcaactatac taaggagggt aaaataatga gagttaatac
    23821 aaatgtaagt gctttgatag caaataacca aatgggaaga aacgtaaatg cacaaagtaa
    23881 gtctatggag aagctatctt ctggtgttag aattaagaga gctgctgacg atgctgctgg
    23941 gcttgctata tctgagaaaa tgagagctca aataaaaggt ttagaccaag caggaagaaa
    24001 cgttcaagat ggtatatctg ttgtacaaac agcagaaggt gcattagaag aaactggtaa
    24061 catactacaa agaatgagaa ctttatcagt acaatcttca aatgaaacta atactgctga
    24121 agaaagacaa aagatagctg atgagttact acaattaaaa gatgaagttg aaagaatatc
    24181 aagctcaatt gagtttaatg gtaagaaatt acttgatgga agttctacag aaataagatt
    24241 acaagttgga gcaaactttg gaacaaatgt tgcaggaaca tctaacaaca acaatgaaat
    24301 aaaagttgct ctagtaaata cttcaagtat aatgagtaaa gcaggtataa caagttctac
    24361 aatagcaagc ttaaatgcag atggaacttc tggaacaaat gcagctaaac aaatggtatc
    24421 tagcttagat gtagctctta aagaattaaa tacatcaaga gcaaaattag gggcacaaca
    24481 aaatagacta gaatcaactc aaaacaactt aaataatact atagaaaatg ttacagcagc
    24541 tgaatcaaga ataagagata cagatgttgc ttcagaaatg gttaacttat ctaaaatgaa
    24601 tatattagtt caagcatcac agtcaatgct tgctcaagct aatcaacaac cacaaggagt
    24661 tttacaatta ttaggataaa ataattttgt atattggata tagaagaaag ggtaaggctt
    24721 tgctttactc ctttcttttt ttacaaatat acttttgaaa ggaaaattta tatgttactt
    24781 agtatagtta tgattgttaa aaatgaagaa aaaatacttg aaaaaacttt aaaatcatta
    24841 accacattaa gaaattctat agaaagtgaa cttataatag tcgatacagg ttctacagat
    24901 gatactataa aaatatctaa gaagtacacg gaaaaagtgt attttcataa ttggaataat
    24961 gatttttcta gtatgagaaa tatatcaata tcctatgcta aaggtgaatg gttacttata
    25021 ttagatgcag atgaaatact aattgattct tcaactattg taaaattttt taaagatggt
    25081 cttgataaaa aatttaattc tgcttcaata caattaaaaa atctttactc atatgataaa
    25141 aaattatatg gatattgttc agtgcttaga ctttttaaaa acgttgattt tagataccat
    25201 ggtaaagtac atgaacaacc aatatacaaa aaccctatat ttaataatgt tgctgatttt
    25261 gaacattatg gatatttgtt tgaagatgaa gaaataagat taaataaggt gaaaagaaat
    25321 gaaaaactat tatttcaaga gttaaaagag aatgataatt ctccatatac aaactatcaa
    25381 ttagggaaaa attttattat attaggaaaa tatcaggagg ctttatacta tcttgagaaa
    25441 tcttatgaat tatattctaa actagaattg gcacctggtt acttgataac aagccttgta
    25501 aaaacatatt tatatttagg aaaacataag aagtgtgaaa gattgtgctt aaagtatatt
    25561 aagaaagaca tgaataatat tgatatatat tattatattg cacaagcaca agtagattta
    25621 ggaaagtatg aaaatagtat agattcgtat aagagatata tttacttgtt agataattat
    25681 gaaataagta cacaagcaaa tagtttattt tcagacactg atactgtagg gcttagagat
    25741 gaggctatta ttacactgat aaagatttat tataagcttg aaaaatatga gtttgtaatt
    25801 tcagagtata ataacattga agatgtggaa aagaaaaaga atgtgtactt tagcttattt
    25861 atgtcactat ataaattgaa tagatttgaa gatataaaaa attattataa agaagtttca
    25921 aattctaaaa ttgaaaaaaa tagtttttat agaaatatag aagaagttat aaataatatt
    25981 aaagaggatg aaaaagagta tgtatataaa atcctttgtg acattgaagg taattatggc
    26041 attttaaatc aaataaggat aaataaatgt gtatctatag ataaatgtaa agaaatactc
    26101 aatgaagaaa aggaaataat ttatgcatat attataaaaa tagcttttga agagaagatt
    26161 gatttactag atatttttta tgaaatggaa tatgtatgga tagagaaata tctaaaatat
    26221 cttttagcat tagaaagaag ttttaattta gtactatata agtacataat tagtcgtcca
    26281 aatacatcag atatggaaaa gataagagta tataaagcta tatctaaagt agttttagaa
    26341 agtataagct taagtgatga aaaatataag gagatttttt atctttatat aatgtattct
    26401 tataagtaca taagatacat atatagcaat ttaagtgatt ttgaattaat aaaatttgtt
    26461 tgtaatgatt tagataagtt cactatagaa tttaagcatc tacttgatat taaaaataaa
    26521 gtaaataatg ctaatgaaaa attagagtat atacatagta tgaggaaatt actaaatgag
    26581 tatccttttt ataataaaat aataaaaata cttataggtg aattagaaga aaatataaaa
    26641 gaaagtgaag aatttaagga tttaaaagat agttttatta ttagtataga aaatatgatt
    26701 gaaactggca aaataaatga tgcgaaatta ttggtagatg actattcaaa aacatttgtg
    26761 gatgatataa agattttaaa tataaaggga attttataca tgtttgaaaa taagcttgat
    26821 aaagctgatt ttatgttaaa aaaggcatta tcattagatg tagaaaatga agatactata
    26881 tataatatag aatacttaaa aagtttaaga taagtcaaaa ataggagaat tgaagaatga
    26941 atataccaat agtggttaat gaaaaatcta aagacataat aaaagattgg caatatacat
    27001 gtacaaatga agatataaaa tttacaagca taataatatt gacatataat caaatagaat
    27061 atactaaatt atgtattgaa agtattagga aatttacccc aaagaataaa tatgaaataa
    27121 taataattga taataactct tctgatggaa cagttgagtg gttaaaggga caatctgata
    27181 taaaatctgt gtataataac aaaaacttag gatttccaaa aggatgcaat caaggtattg
    27241 agttagctac tggtgaaaac atattgttgt taaataatga tgtaatagtt actccaaatt
    27301 ggctctataa tttgaataaa gctttatggt ctcaagataa tataggtgct gtaggaccta
    27361 ttacgaatgc atgttcatat tatcagcaaa ttgatgttga ttataagtca attgaggaca
    27421 tgtttatatt tgcacacaaa ataaataaat ctaatattaa cctatggaaa tatagattga
    27481 agttagttgg attttgcatg ttaataaaaa aaactgttct ggataatgta ggggttttag
    27541 atgaattatt cacacctgga aattttgaag atgatgattt atcttttagg atgatagagt
    27601 caggatataa gttattatta tgccaggata cttttataca ccattttgga agtgtgtctt
    27661 ttaaagaaaa tcgtaataaa tatacagata taatgtatat taatagaaga aaatttgaaa
    27721 ctaagtgggg atttaatcca gaatatagtg gaatgataag aacagatttg atttctaaaa
    27781 tagatgatag tttggaggca aatattaatg ttttagaaat aggatgcggt ataggggcta
    27841 ctcttttaga aataaaaaat atatataaaa atgcatcaat atatggaata gaaattagtg
    27901 agtcagcagg taaacttgca aagtatagtg cagatgtact tatagcaaat atagaaaaag
    27961 taaaattgga ttataaaaaa gatttttttg attatataat attgggagat gtacttgaac
    28021 atttgattga cccatggaag atagtcaatg aattgaaaca atatctaaag aaggatggtt
    28081 atctggtatc tagtatacca aatattatgc atgtttctgt tttaagaaat cttgtgttag
    28141 gtaattttac atatgaagat tcaggaattt tggacaagac tcatttaagg ttttttacat
    28201 taaatgagat aacaaaaatg tttaatgata gtaattacat aattaatagt attacagcta
    28261 ctagtatgcc actttcggaa attgataata gcttcattga tacaatatgt aaggaaagct
    28321 gtaaagaaat gagacaacag tacgaatctt atcaatatat agtaaaagcc tctaaaatcc
    28381 tagatactaa aagatattct agtattagta tgattaattt aaaatacgct ttaatgagaa
    28441 ttgataatga attagatgta gatagtaatt taagttatat atttgataat tataatataa
    28501 ctgaaccatg ttttgttgat gatattgaat atctaattga tacatttgtg ataaataaag
    28561 caactctatt aaatagatta ggagttgagg catttaacag aggtctagga gattttagta
    28621 taaacatgtt tatgacagta ttagagatgg atagagatga tattgactca atatataata
    28681 taacaagtgt attatgtgaa ttagaagaat ttgatactgc ttataattta attaaaaata
    28741 gtagcgaaaa agtacagagt gaaagtgata tacaagagat actttgtttt attgagggga
    28801 agaaatatga ataatgataa gatatgtttt ataacctgtg taaatgatga aaggctttat
    28861 gaagaatgtc ttgtatatat aaatagccta gacattccta aaaactttag tatagaaaca
    28921 gtagctttaa gaggagccga ttctattgcc tctgcttata ataaagctat gagagaaaca
    28981 gattctaagt ataaggtata cttacatcaa gatactttta taattaataa aaatttcata
    29041 catgatattt tagagatatt taaaaaaaat acagatgtcg gaatgattgg tgtagctgga
    29101 gtaaaagata tgcctgtgaa tggaatttgg tgggattctg ataaaaaagc agggaaagta
    29161 tatgatagtc atacaggtaa aatggatatt tatgaattta gtaactttga agaatgtttt
    29221 aatgaagtta tggctataga tggattaatt atgattactc aatatgatgt attttggaga
    29281 gaggataagt ttgatgggtg gcatttttat gatttgtcac agagtatgga gtttagacaa
    29341 aataaataca aagttattgt accaaaacaa aataagcctt ggtgtatgca tgattctgga
    29401 gtagtaaata taaataatgg atatgatgaa tataggataa aatttgtaaa agagtacata
    29461 aatcttgatt tatatagaga aaatttttat gaatttgata agaagaaagc aagtaccata
    29521 cttaagagta ataagcatgt aatggattgt atgaaagagg agcttacaga acaattaaga
    29581 ttaaagaatt acaaggatgt tttaggtatt gtttctaaaa ttgctcattt ttctgttttt
    29641 aatcatactg gtttgtattc ttgtgaagaa tgtgagagag cattgctaga atgtgcaaat
    29701 aatttaccag aaatagaaaa tacgaaatta tttacaaaaa aagatttaaa aagaagaaaa
    29761 gtattacatg ttttaagtga aggatattca acaggaggtc atacaagact agttaaaaat
    29821 tggattgaaa ttgacaaaga ctctgttcat tcagttgtaa ctacatggca atcagataca
    29881 ctgccagact ggttattaaa agcaataaat gatagtggtg gcgaagttat atctttggat
    29941 ttaatttcag atgattattt aaaaagagct gtagctttga ggaaaatagc ttatgactgg
    30001 gctgatatag ttattttata tgttcatatg tgggatacaa tacctgttgt tgcttttgga
    30061 gttgatggga aaatacctat tttacatgtt aatcactatg atagtacttt ttggttaggg
    30121 acaagtattg cagatattgt cattgatttt agcgaagcta gtcaattaat tacattagat
    30181 aaaagaggca tagataaatc tgaaatatta ccaatgccat taaaagaaaa agaagtattt
    30241 tcaaaatctg aagttagaaa acgatataat ttagaggatg atgtgacaat aatattgact
    30301 attgctagtg aaagaaaata caatagtgta aatgatatta attactttaa aattgctaaa
    30361 acattgattg aaaatacaga taatacaatt attattgttg taggagttga tacagagaat
    30421 ctctattgga aaactttaag taacgttaca gataatagag ttatatgttt tggaacaaca
    30481 gcaaatatag acgattttta caagatttcg gatatatata tggagtcttt catggtagga
    30541 agtcatactt caaaactaga tgcaatttta tatgatttaa ttcctgttaa gtttaaaaat
    30601 gaagtttcac caatttttac aaatatgtgg aattttatag ataagtttga ttataagaat
    30661 ataaaagagg ttatagatat gataaaaagg tacaatacta gtgaatttga cagggatagg
    30721 ataaaagaaa aacaatcgga aataaaacat tttgttatag ataaacactg tattgaaata
    30781 aaaaactata tagacaaaat atataatgaa gctaatcatc ataaagttaa gtatgattta
    30841 tataaaaatg atgaaatcga ggactatcag ctttttgtag cattatatgg ctatatggta
    30901 aaaaataaaa acatgtcata ggtggtaaaa tgaacaatat tcatatcaat aaatttgaaa
    30961 aaagctgtga aagaaactgg gattattttg tagaaaataa atctataaat ggtacatttc
    31021 tgcaaactag aaatttcctt aattatcatg aggagggaaa attcactgac cactcattaa
    31081 taatatgtaa aggtgaagaa atactagcag tcgtacctgc atgtgaaact tatgaagatg
    31141 gtgaaagaat atttttttct catttaggga gtacttttgg tggtattata ataagaagtg
    31201 gttttaatga tattaggcat gtaaatgcca ttacaaataa gttagaaata tatttacggg
    31261 aacaaaattt cgataaagta tatttaaaaa atacatcaag aatattttgt aaagaggata
    31321 cttcattact agaatatttt ttatttaaga ataattacat gttttatgat gaattaagta
    31381 tgtttttaga attaagtaag tataaagaag atatattagc caattttaaa tacagtaaaa
    31441 gaagagattt taaatattct ttgaagaata atcttacctt taaaagactg tatagtgatg
    31501 aagatatagt ttgttttttt aatttacttt ctaattgttt agatagtaaa ggagctaaag
    31561 taatacattc agtagaagaa cttttggagt ttaaaaataa tagattaaaa gatattgtag
    31621 aattttatgc agtatatatt gatgacatta tgatttgtgg gagcatggta tttaactttc
    31681 aagaaagagt ttatcatact caatatctag catcaaattc gacatataat aaatattatc
    31741 caatgatatt tttatactat aatctaataa aagatgcttt agagagaaat tttaagtatc
    31801 tttcttttgg aattagcact ctaaatagag gcaaatactt agacgagggt ttagctacat
    31861 ttaaagaagg ttttggttca agtgcaattt gtaacagaac atactataaa aatataaata
    31921 agtgagaatc atgttttata gagtttttag aatatagtaa atatctagta ctctaacatt
    31981 acataaaaat aattagttta taaagaaggt ttgtatatga aaagattaat gattttaggc
    32041 tcaattaagt attttgagaa tattgttata tcagctaaaa aagagggaat atatacgatt
    32101 gtttgtgata atagaattga tactcctgcg aaaaagattt gtgatgaagc aatagatgtt
    32161 gatgtatttg attttgataa aataaaaagt atagctatag ataaaaagat agatggaatt
    32221 ttaactggat ttacagattc tttaatgaaa ccatatgttt atattgcaaa tgaactaaat
    32281 ttaccatgtg tgatttcttg caatcaactt gaatctgtaa ctaacaaggc tattatgaaa
    32341 aaatatttta aaaataatgc aatacctaca actgattact gtattattga aagtctaaaa
    32401 gatttagaaa aaatacaagg attaaatttt ccacttgtaa taaaacctgt tgatggttat
    32461 ggttcaagag gtgtatattt tgtatcaaat atagaagaat taatagaaaa atttgaatat
    32521 tctaaggaag cctcacaaga tggacaagta ataattgagg aattttatga aagtgaggaa
    32581 atacaaggac tagcttgggt tcataatgga gagtcacatg tattttatat tggagataga
    32641 gagcttgtaa acatacatac aggaagagct ggaaaaccag atagattact ttatccatca
    32701 aagtattgtt ttcaatatga ggaggaaata aaatctatat atcaaaatat aacacaggct
    32761 tttgatataa aaaatggacc tctttatgta caaatgttag tgggagcata tggaataaag
    32821 gtatctgaag taatgccaag attacctgga ggttgtgact atatggctat ttctcaaata
    32881 acagatttag acataggaag tttatttgca aactttagtg tgaataatga agtaaatttt
    32941 gatgaaataa agaaatataa tatgcatcta tcaaaatgtg tatatgcatt accaatatac
    33001 ataagacccg ggacaataaa aaagataaat aatatagata aaatagaaaa cttagattat
    33061 gtcactcaat ttctattact agtaaaagaa ggagatacaa tagaattata tgaagatgag
    33121 agacaggatt gtggaagagt ttatggaata gcaaataata tatttgatgc aaatataaaa
    33181 aagaaatgta ttcatgaaat gatagaaata cttgatgaaa ataatgaaaa tatgatagaa
    33241 aatttttaaa taagggagta tgatagatat gaagctaata actcatgaac aaattatgtc
    33301 attaaacatt tctcagaaac aaggatatga gtggataaaa gaatctttct taacaaaaaa
    33361 agattgtcaa ttaccaccaa aaataagcat ggtagaagaa ggacatgtat tttacaatgt
    33421 aatgccatcc ctaaatcata atcatatgat tggtggaata aaaatggtta caagattgcc
    33481 agggagaaat ccaagtttaa actcagagat atttctatac aatatggaag atgctaaatt
    33541 aaaggctatt ttggatggta attatattac agcttttagg actgctgctg ttgctgcctt
    33601 ggctatcgat acatttgcta aaaaagattt taatactatt gctattatgg gtttaggtgt
    33661 aactgcttct gctataatga atatgctttc atatactctt aaagataaaa atataaatat
    33721 aaagttactt aaatataaag accaagcaga gttgtttgta aaaaagtatg aaaaaaatga
    33781 acaatttagt tttgaaatat gtgatacaca tgaagaactt attgtagatt cagatgtagt
    33841 tatttctact gtgacatata cagatgaata tttatcagaa tttaaatggt ttaaaaaagg
    33901 tgctttatta ttacctgtac atgttatggg atttggaaat gttgattatc tatttgataa
    33961 agtatattta gatgattata atcatttgaa acatctagaa aatatagata gatataaata
    34021 ttcttgtgaa ttttatgatg ttttaagtaa acaagctaaa ggaagagaaa atgatgatga
    34081 gagaataata tcttacaata taggtttgtc aatacatgat atattatttg caaataaaat
    34141 ttacgaattg tgtgaaacac taagctgtga agaaatagaa ttaaaagaac caaaagaaaa
    34201 atattggata tagtttattt cattcatatt tacaatagtt tattgtaaat atgaatatga
    34261 agtaatatat tgttagaaaa ttagcatgaa taggatagat aaatttatgg ataaatataa
    34321 tttaattagg ttcgtatcta ttggaaacga atatatagga aatttagttg cattagaatc
    34381 aaataaagta ataccttttg atataaagag agtttattac atatatgatg taccaaacaa
    34441 tgtaaagaga ggatttcatg cacacaaaaa cttggaacaa gtactagttt gcctaaatgg
    34501 aagtgtgaag ataaaatgtt atgatggtgt aaaagagact atatttaatt tacataaaaa
    34561 tgatgttgga ttatatatag gaacgggagt gtggcatgag atgtttgatt ttgatgaaaa
    34621 aactgtttta atttctatag catctgaaca ttataatgaa gaagattata ttagagacta
    34681 taaagaattt ttaaagtatt taaataatgt gtagttatta tacaattagt gatgtatttt
    34741 cttaattaaa taaatttaag atagccacat agagaataga tagattagga tgtgaattta
    34801 ttgttggtta aatttaataa tttattaaaa cagttccaaa ctcataaaga agaatatgaa
    34861 cagaagatta aggatgtaat ggaaagtgga tattatatcc ttggaaatga agtaaagcat
    34921 tttgaagaag aatttgctaa ctatgtaggt agcaaatatt gtgttggttt agccagtggt
    34981 ttagatgcac tgataattgc ttttagagca ttaggaataa gcaaaggtga tgaagtaatc
    35041 gtgcaatcaa atacatatat tgctagtgtt atgggaatca gtataaatgg agcaacacca
    35101 gtatttgtag aaccaaatga atattataat atagattcat ctaagataga agaaaagata
    35161 aatagcaaaa caaaagctat attagtagtg catttatatg gtcaagcaag taatatgaaa
    35221 gatatacaag aattatgtga taagtataac ttgtatttaa ttgaagattg tgctcaatct
    35281 catggagcta aatatgatgg aaaaatgaca ggaacatttg gtgatatagg ttgttttagt
    35341 ttttatccaa gtaaaaattt gggtgcattt ggagatgcag gagctatagt aactagtaat
    35401 gaggaaatag ctgataaagt gaaaattctt agaaactatg gctcagaaaa gcgatattat
    35461 aataaatttg tcggatataa ttcaagattg gacgaaattc aggcaggttt attaagagta
    35521 aaacttaaat atctaaatga aattgaaatt gaaaaatcaa aaatcgtaga tagatatctt
    35581 aatgaaatta ataatcccaa agtcatactt ccaaagatag aaaataattg tactcatgta
    35641 tggcatttat ttgttttaca agttgaaaaa cgagatacat ttatggaata cttaaagaaa
    35701 agtggtatag agtctataat acattatccg ataccaccac atctatctga agcatatgct
    35761 tatttgggat ataaaaaggg gagttttaat atagctgaga actattcaga taaagttgtt
    35821 agtatacctt tatttattgg tatggaagat gaagaagtta gttatataat taaaattata
    35881 aatgaatatt aaactactca taataatatc aaaaataaat tttttatata tcatttattt
    35941 aaatt
//
LOCUS       DAYATN010000025        35482 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.025, whole genome shotgun sequence.
ACCESSION   DAYATN010000025 DAYATN010000000
VERSION     DAYATN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35482
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.025"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..664
                     /locus_tag="ACGI8A_001222"
     CDS             <1..664
                     /locus_tag="ACGI8A_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179225.1"
                     /translation="GEFVSIIGSNGTGKSTLLNIISGLIKESSGQVLLNKHNLSNLAE
                     HRRTQIVSRVFQDPSLGTCPSMTVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGV
                     SLDLKKYLDVQVKFLSGGQRQSLSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTN
                     KIVREKNITTLMVTHNLKHALHYGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEY
                     AV"
     gene            795..1817
                     /locus_tag="ACGI8A_001223"
     CDS             795..1817
                     /locus_tag="ACGI8A_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD0873 family tyrosine ABC transporter
                     substrate-binding lipoprotein"
                     /protein_id="HFH0179226.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1981..2538)
                     /locus_tag="ACGI8A_001224"
     CDS             complement(1981..2538)
                     /locus_tag="ACGI8A_001224"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFH0179227.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2544..3620)
                     /locus_tag="ACGI8A_001225"
     CDS             complement(2544..3620)
                     /locus_tag="ACGI8A_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFH0179228.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3621..4304)
                     /gene="modB"
                     /locus_tag="ACGI8A_001226"
     CDS             complement(3621..4304)
                     /gene="modB"
                     /locus_tag="ACGI8A_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFH0179229.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4453..5265)
                     /gene="modA"
                     /locus_tag="ACGI8A_001227"
     CDS             complement(4453..5265)
                     /gene="modA"
                     /locus_tag="ACGI8A_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0179230.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5458..6387
                     /locus_tag="ACGI8A_001228"
     CDS             5458..6387
                     /locus_tag="ACGI8A_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFH0179231.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6548..6997)
                     /locus_tag="ACGI8A_001229"
     CDS             complement(6548..6997)
                     /locus_tag="ACGI8A_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179232.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7200..8072)
                     /locus_tag="ACGI8A_001230"
     CDS             complement(7200..8072)
                     /locus_tag="ACGI8A_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0179233.1"
                     /translation="MYNSSVGLEQLIKNEDIVKIFSERKDRPLEKLKNYKTSELMIQS
                     SNNYKLESLFITSNIKTRDTMILVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGN
                     SGGDNYTFGLYERYDLDSLVKFVKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVD
                     FYILDSPYSEMKDAIRMGVLEKRIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVE
                     KSNVPILFIHGTKDTVCNYQNSKKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNK
                     IFKFIDSNVLSDKS"
     gene            complement(8385..9239)
                     /locus_tag="ACGI8A_001231"
     CDS             complement(8385..9239)
                     /locus_tag="ACGI8A_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HFH0179234.1"
                     /translation="MKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKEVP
                     SKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDIEE
                     IEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCDKI
                     IKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLNTI
                     KEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEEEE
                     EKYRRIFK"
     gene            complement(9562..10890)
                     /locus_tag="ACGI8A_001232"
     CDS             complement(9562..10890)
                     /locus_tag="ACGI8A_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFH0179235.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(11038..11367)
                     /locus_tag="ACGI8A_001233"
     CDS             complement(11038..11367)
                     /locus_tag="ACGI8A_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFH0179236.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(11391..12680)
                     /locus_tag="ACGI8A_001234"
     CDS             complement(11391..12680)
                     /locus_tag="ACGI8A_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0179237.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(12696..13022)
                     /locus_tag="ACGI8A_001235"
     CDS             complement(12696..13022)
                     /locus_tag="ACGI8A_001235"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0179238.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(13157..13786)
                     /locus_tag="ACGI8A_001236"
     CDS             complement(13157..13786)
                     /locus_tag="ACGI8A_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179239.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(14001..16658)
                     /locus_tag="ACGI8A_001237"
     CDS             complement(14001..16658)
                     /locus_tag="ACGI8A_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0179240.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(17017..17961)
                     /locus_tag="ACGI8A_001238"
     CDS             complement(17017..17961)
                     /locus_tag="ACGI8A_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179241.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIDVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(17954..18979)
                     /locus_tag="ACGI8A_001239"
     CDS             complement(17954..18979)
                     /locus_tag="ACGI8A_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179242.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(19054..20622)
                     /locus_tag="ACGI8A_001240"
     CDS             complement(19054..20622)
                     /locus_tag="ACGI8A_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0179243.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(20735..21646)
                     /locus_tag="ACGI8A_001241"
     CDS             complement(20735..21646)
                     /locus_tag="ACGI8A_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0179244.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(21630..22616)
                     /locus_tag="ACGI8A_001242"
     CDS             complement(21630..22616)
                     /locus_tag="ACGI8A_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0179245.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            22934..23377
                     /locus_tag="ACGI8A_001243"
     CDS             22934..23377
                     /locus_tag="ACGI8A_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFH0179246.1"
                     /translation="MILLKSNYDCFRIAMLLKELYSKTMYTVEENFKENGLTHQQIIV
                     IKLIAHNQELTISQLCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTT
                     GFEFATNFKIKMQESFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            23380..23778
                     /locus_tag="ACGI8A_001244"
     CDS             23380..23778
                     /locus_tag="ACGI8A_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HFH0179247.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(24228..24452)
                     /locus_tag="ACGI8A_001245"
     CDS             complement(24228..24452)
                     /locus_tag="ACGI8A_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HFH0179248.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGVTMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(24487..25854)
                     /locus_tag="ACGI8A_001246"
     CDS             complement(24487..25854)
                     /locus_tag="ACGI8A_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0179249.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(25938..27473)
                     /locus_tag="ACGI8A_001247"
     CDS             complement(25938..27473)
                     /locus_tag="ACGI8A_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HFH0179250.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EVSLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(27496..27930)
                     /locus_tag="ACGI8A_001248"
     CDS             complement(27496..27930)
                     /locus_tag="ACGI8A_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6323 family protein"
                     /protein_id="HFH0179251.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(28190..29101)
                     /locus_tag="ACGI8A_001249"
     CDS             complement(28190..29101)
                     /locus_tag="ACGI8A_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HFH0179252.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            29397..29750
                     /locus_tag="ACGI8A_001250"
     CDS             29397..29750
                     /locus_tag="ACGI8A_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFH0179253.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            complement(29969..30910)
                     /locus_tag="ACGI8A_001251"
     CDS             complement(29969..30910)
                     /locus_tag="ACGI8A_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HFH0179254.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(31011..32246)
                     /locus_tag="ACGI8A_001252"
     CDS             complement(31011..32246)
                     /locus_tag="ACGI8A_001252"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFH0179255.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            complement(32290..33144)
                     /locus_tag="ACGI8A_001253"
     CDS             complement(32290..33144)
                     /locus_tag="ACGI8A_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179256.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            complement(33272..34447)
                     /locus_tag="ACGI8A_001254"
     CDS             complement(33272..34447)
                     /locus_tag="ACGI8A_001254"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HFH0179257.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            complement(34546..35430)
                     /locus_tag="ACGI8A_001255"
     CDS             complement(34546..35430)
                     /locus_tag="ACGI8A_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFH0179258.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
BASE COUNT        11536 a         6223 c         3784 g        13939 t
ORIGIN      
        1 tggtgaattt gtaagtatca taggtagtaa tggaactggt aaatctactt tattaaatat
       61 aatatccggg cttataaaag aatcttctgg tcaagtatta ttaaataaac ataacttgtc
      121 taatttagct gaacacagaa gaactcaaat agtaagtaga gtttttcaag acccaagtct
      181 tggaacttgc ccttctatga ctgttagaga aaacttgtct ttagctctta acaaaggaaa
      241 actattaaat ctaagaaaat gtcttcgtta caaaagagat ttcttagaaa atttattgga
      301 aggtgtatct cttgatttaa aaaaatacct tgatgttcaa gtaaaatttt tatcaggagg
      361 tcaaagacaa tcattatcac taattatgtc gtgcttaaca aatccatcag ttttattact
      421 tgatgaacat acagcagcac ttgacccaaa aacatcaaat gaagtaattg aattgactaa
      481 taagattgta agggaaaaaa atataactac acttatggtt actcacaact taaaacacgc
      541 tcttcattat ggtgatagat tgataatgct tcataagggt gaagttgtat tagatgtaaa
      601 aggtaaagaa aaagaacaat taacagttga agaaatctta gaaaaatttg aatatgcagt
      661 ttaaaataat atatctattg tattaaactc ggctttaaaa taaagttaaa attacaataa
      721 ttatggttac tttgtaataa ttcatatgaa tatatgaaca tatataacaa taaatttttg
      781 agggggatat aaaaatgatt aataaaaaaa gattggcaag tttgatttta gctggtgctc
      841 ttagtatatc tatgcttaca gggtgttcac aaggaggaga ctctggtaat tcaaaacaag
      901 aaagtaattc taaaggtaaa gaagttaaaa aaattggtat aactcaactg gttgaacatc
      961 cagcattaga tgccactagg acaggttttg taaaagcatt agaaaagaac ggatttaaag
     1021 atggagaaaa tattgatata gacttccaaa atgctcaaaa tgacatgcct actacacaaa
     1081 gtattgcgag taaatttgca tctgataaga aggacttaat atttgctata tctactccat
     1141 cagctcaggc agcatttaat gctactaaag atatacctat acttattact gctgtgagtg
     1201 accctgttgc tgctggatta gttaaaactc ttgaaaagcc aggcacaaat gtatctggaa
     1261 catcagattt tgtttctgta gacaaaggtt tagaacttct taaaatattt gctcctaagg
     1321 caaaaactat aggagttatg tacaatacta gtgaagtaaa ttccaaagtt caagttgatg
     1381 ctttaaaaga atatgcatct aaaaatggat ttaaagttgt tgaaaaaggt ataactactt
     1441 caaatgaagt taaccaaggt atttctagtt tagttggcaa aatagacgta ttatatgtcc
     1501 ctacagataa cttagtggct tcttctatgc ctatagtctc taaaatagct actgagaata
     1561 aaatacctgt tatagcagct gaatctggtc cagttgaaaa aggtgctctt gcttgtcaag
     1621 gtatcaacta cgaaaaacta ggttacaaaa caggtgaaat ggcagttaaa attttaaatg
     1681 gcgaatcagt atctgatatg cctgtggcta catcagatga tacagatatc attgttaatg
     1741 aagatatatt aaaagcttta ggtatggaaa aaccttctaa tgaaaatatt tcttatgtaa
     1801 agactaaaca agaatagaaa gatattttct atatacgatt aatatgcctg tttctataaa
     1861 ttaatttata gaaacaggca tatattttta ttttaatgta ttatctattt aaagattata
     1921 tgtaaatgct cttaatttgt ttgtataaat tttacttgta taaactttat acaatcatat
     1981 ttattcttct attttcttta ttactctaca tggatttcca acagctacag tatttggtgg
     2041 tatatctttt gtgactacac ttcctgcacc tattactaca ttatcaccta tagtaattcc
     2101 tggtgttatg attactcccc cacctatcca tacattatca cctattttta taggagaacc
     2161 atactctatg cctgaatttc taagttgtgc atcaattgga tgataggctg tatagatttg
     2221 aacgtttgga gctaacatta cattgtcacc tatttcaatt ttacatacat ctaaaaaaat
     2281 acaatcataa tttgcaaaga aattttcgcc tacatgtatg ttatatccat aatcacacct
     2341 aatattttgt tctacattta tttgcttgcc aactgaacca aatagttgtc ttaaaatatc
     2401 ttctcttttt tcatattcat cctctaaagt attattaaac aatctagtta attttttaca
     2461 atactctctt tcttttacta acaattcatc atttgcatag tatcctttcc cagacaacat
     2521 tttttctttt tcagtcatat agtttactcc tttttgttat aattgtatct aaagcaaaat
     2581 aaatattcct gtggaaaatc taaatatatt ctgtcacctt tcttaaatct cattttactt
     2641 ttttccaatt ctatttgaat aggtacacat tcgttagata aatcagtttt tctaacatat
     2701 atagtatatg taaaaggatt ttctacaatg ttaatcaatt cgaaacataa cttttcattt
     2761 aaatcttcat tagaatccaa gaccttaata tgatgctccc tgattcccac atattctata
     2821 ttatcaccta tttctctatt taatgtcaac tcgcatcccc agtctgtagc ataaatagta
     2881 tttttatcca gtctatttaa tttggatata tttttacacc ctgttatctt agcttctata
     2941 aggtactttg gagattcaaa gagctctttt ttaggtctct ttggaagacc ttctcctttg
     3001 ttgtacacta taatatcatc acaaactcgg taagcttctt caatatcatg agtaacaaat
     3061 agtatatctc ccttgtaatc ttttaacata ttcataagtt ctttttccat attgcttctt
     3121 aaatgatggt ccaatgctga gaaaggttca tctaaaagta atatatctgg agatgttgcc
     3181 aatgctctag ctagtgctac cctttgttgt tgaccaccag ataattgcca agggtatctc
     3241 ccctcaaacc catttaattt aagtatatct aagtaccttg ctactatttc ctttttttca
     3301 ctcttactta gttttaaaag cccaagttct atattttgac ttacagtcat atgtggaaac
     3361 aaagcatagt tttgaaataa aaatcctacc tttctatctt gtgttgataa atttatcttc
     3421 ttttcagaat caaatagaac tctatcattt aatacaattt tacctcttgt tggtttctca
     3481 agccctgcta tacattttaa tgtcatactt tttccagaac cagattctcc caaaaacccc
     3541 agagtacctt tttcttgttt aatttctacc tttagtttaa aagaggataa atctttttca
     3601 atatccacgt ataagctcaa ctatccacac ctctttccta taattttctg ctgaaattcc
     3661 gaccaataat ttagtaataa aatcattgct atagatattg ctacaataat cataacccac
     3721 atcatagctt tattcatatc tccgccttca acagcaaaaa atatagctat tggcatagtt
     3781 tgagtttttc caggtatatt tccagctatc ataagtgtag caccaaattc tccaagagct
     3841 ctggcaaacg acagcaccaa tccaccaatt attccaggcc atgctaatgg aactgcaatc
     3901 ttaaaaaata ctttccattc attaagacct aatgttctag cagctgacaa catattatta
     3961 tcaatctgtt caaaagctga ccttgaagtt ctatacatca taggaaaaga caccactact
     4021 gctgaaatta cagttgctgt ccaactaaat attaaagtcc tatcgaaatt taataaaaac
     4081 tttcctataa atccattctt cccacatagt aataataaaa agaaacctac aactgttggt
     4141 ggcaaaatta gtggaagtgt aaatagtcca tctataagac ccttccactt acctctatag
     4201 ttagccataa tatatgacac aaatattcct attacaaaag ttattattgt tgacaaaata
     4261 gatgtcttaa gtgatatcca taagggtgac caatctgttc ccatctttcc ctccttaatt
     4321 ttaaatggaa atagtgaaac taataactct attaatttca ctatttccat tttattataa
     4381 tacacaatta ttttcaattt tcatttattt atatataatt tatacttaat tacacataat
     4441 ttatttctaa gtttattcta cttttttata tccaaattct tcaaaaatct tttgtcctga
     4501 ctcacttaat aaaaattctt caaatttctt agcagcatct acgtttttac tagctttaat
     4561 tactgaaact ggatatgtaa taggcgaatg agttttttcg tctgtttttt caactacttt
     4621 tatcttatca ttattgacag tatcactgaa gtaaacgaat ccaacatcag catttcctga
     4681 ttgcacccat gctaaaactt cttttacatc ttttgcaaat actagcttat cttttaactt
     4741 atcttttaaa tttaaattag ttaacacctc atctgcatac ttaccagctg gaacactttc
     4801 agcttcacca acagcaattt tcttcacttt atctgtagtc aaatctttca tacctgatac
     4861 actactgtca gcagaagaaa taagaactaa atcatttttt actaaatctt tcatagtatc
     4921 agaaactaat aacttctctt catccaaagc tttcatttgt ttttgacctg ctgaaataaa
     4981 taaatcacaa ggtgctcctt gttctatttg ttgttgtaat gagcctgatg ctccatagtt
     5041 aactgtaagt tttacatttg aatcaacttt tttatattct tcttcaatct ttgccattgc
     5101 ttcttttaaa cttgcagcag ccgatatatt taactctact gaatcactac ttttagttgt
     5161 tttttcttgt tttccatcat cttttttgtc ttcattacta ttgcacccaa ctgtccccaa
     5221 agttaaacat gcaactaatc ctaatattcc caatattttt ttcataacaa tctccctttt
     5281 tgtaaatttg atacacattt taacatgttt tattatgttt tatcatgttt tgttatatat
     5341 agtttaatta tatatacatg ataataaatt ttcaagtaat ttatttacac tacttttaaa
     5401 tattttgcta cattttcctt tttttaatgt taaaataacc ttatatggag gtggattatg
     5461 gactcattat catctttgac acctgtcgag gtagctgaat tattaaagat aacaaaaaat
     5521 actgtatatg aattaatcaa aagaggtgaa cttccttctt ataaagtagg gaaaaaaata
     5581 agagttgata ctaaagatgt tgaagaatat atcaacagcc aaaaaactgg taaagtaaag
     5641 gatacacatt tcaacaataa aaatatagat ataaataata atttcaaaat tattatttct
     5701 ggtcaagatg taatattaga cattttagct aggtctgttg aaaaaaaatt ggatggaata
     5761 agtacatttc gctcttatat aggtagttat aatgctttgt atgagttata taatggcaga
     5821 gtttctatag catcatccca tctatgggat ttcgaaacgg atgaatataa tagcactttt
     5881 gttaaaaagc tattgccagg aataccatgt gtacttataa atctagctta tagaatgcaa
     5941 ggtttctatg ttgctaaagg aaatcctaaa gagataacaa cctgggagga tttaacaaaa
     6001 ccaagtataa ccattgtaaa tagggaaaaa ggttctggaa ctagggtttt actagatggt
     6061 aaacttagac ttctaaactt taatggtaaa catataaaag gttatgataa tgaagaatta
     6121 tcacatcttg gagttgcaag tactgtttct cgtggaatag cagatgttgg tttgggaaat
     6181 gaaaaagctg ctttacaagt aaataatatc gactttattc ctcttcacaa agagcgctat
     6241 gatttagtta ttaaaaaaga agacttacaa aatccagttt accaaactat aattaacata
     6301 ataaactctc ctgagtttaa agctgaactt caaggtcttg gcggatatga tttaactgat
     6361 actggaaaaa ctatagcaaa agtttaagat taaaatcgca atctatttta taagatttat
     6421 aatttttcac aaaatttata acttaaacac gaaataaatt gcgattttag tgtctcaaaa
     6481 aatttatgtc tttcatttta tcatccaata caaaatataa tataattaat tgtgcaatca
     6541 tattatatta atatatattt acataatagt atttttcttt aatgtagatt aacatatgat
     6601 atgttttaaa catcaatccc cttgctttct tatatatatt tggcaacaca tatgagttta
     6661 ataaattata tacaatgttg ctttcttctt gtattttttc aaccaacaag ttatttataa
     6721 aattaccttt ctcattttca ataatctcac tccaatttat cttaactttt aatatatatc
     6781 ttcccactag gtatacacac tctacaataa ataataaaaa tacaatgcag tcagtacctg
     6841 ttgtcttata taaattaccc gatactaatg gctttgcgaa gaaattataa aaattcaaag
     6901 tatcacccgt ataatcaaaa agtattaata gcactcctag aactctcaat actttaatat
     6961 tcaacatgtt tttatcttta ttatttttgt ttttcatata tactacctct cttttcttta
     7021 gatttgattg taatctaata ctaactcttt tatttgaggt ataaatgaat ttaattggta
     7081 gttattttca tttttactag aaattaattc acattactaa acaaaactta tatttaaaca
     7141 taaaaatagc ctatctattt tttaataaat acaaaaataa ataagctact aatattcaat
     7201 taacttttat cacttaaaac attactatct ataaatttaa atattttatt gaagtatact
     7261 gtactatcat gttcaaatcc atcaacatga ccaatacctt ctataagcca taggtctttt
     7321 ttatcatgtt taactaaatc atacattttc ttgctatttt gataattaca aacagtatct
     7381 tttgttccat gtataaacag tatgggtaca ttagatttct caactgactc atatggtttt
     7441 acatcactgt accaaaatcc agatttaaac ttattatata aatctccaca agtcaccaca
     7501 taattgatta atatatctgg tattcgtttt tctagtactc ccattctaat tgcatctttc
     7561 atttcagaat aaggagaatc cagtatataa aaatcgacct tatcatcttt tgaatttatc
     7621 tctgaatgca tagcagctgt ccctgccccc atggaaaatc catgcactcc taatcttcct
     7681 tcaggaaatt tattttttac aaatttaact agactatcta aatcatacct ttcataaaga
     7741 ccaaatgtgt aattatctcc accactatta ccagtatttc tttgattata aactaatacg
     7801 ttataacctt tatctaaata tctatatgct acctttaaca tttcatagta actacttcca
     7861 attccatgaa caagtatcat agtatctctt gtctttatat ttgaagttat aaaaagactt
     7921 tcaagcttgt aattattaga actttgtatc attaattcag atgtcttgta gttttttaat
     7981 ttctccagtg gtctatcttt tctctcagaa aatattttta ctatatcttc gttttttatt
     8041 aactgctcta aaccaactga actattatac actaatttgc caaatatata agatacaatt
     8101 aataggatta atagaaacac aattgatata attataaatt tatttcttaa actaatacaa
     8161 tttttaaaac tatttttcat atttatttac catacaattt tcctatatta tattttctaa
     8221 gtaatagcat aattgattat actattctta taatttctat tatacctact tatttattaa
     8281 aaatacaaat tatattttat taatttttac tacttgtaaa ttattatctt atacaatgaa
     8341 tttgtattat ataataagta tttttaggat tctgttacat aatattattt aaatattctt
     8401 ctatatttct cttcttcttc ttcagtaaac acattaaata caacttttaa atttttttca
     8461 ggattttctt ttagccatag tctaactcta cttacagcaa gattagctgc ctcttttttt
     8521 ggataaccaa atacacctgt agaaattcca caaaaaacaa tattttttat ttcatcaatt
     8581 tcttttattg tatttaaaca tgatttataa caatgaagca gttgcttttc ttgctcttta
     8641 cttggctgac caccagatac aataggtcca acagtatgta ctacaaattt tgcaggtaaa
     8701 caataacctc tagttatctt agcatctcca gtatattcta aatgcccttg tttttttatt
     8761 attttgtcac aatcttctct aagtctagga cctgcgcatg aatgtatctc attatccacg
     8821 catagatgta atggttgtaa acaccctaat aacttgttat ttgcagcatt tactattgca
     8881 tctgctctta aattcgttat attacctctc catatggcaa tcccttcctc tatttcttct
     8941 atatcattta catctactaa ctctctttct tcacattcaa tcaacaataa attatgtaac
     9001 atattaagta tctctggagc aatttctttt ggtggatata cattcaaaag tcctctaagt
     9061 atatctctag gcttaatttc atcatttgat aaattgtata ttttagaagg aacttctttt
     9121 gaaaaatatg caattaaagt gtttatattt tttcttcttt gttcgtcagt tagaggcttg
     9181 atagttttat caaaatcctc aaataaattt acatcatcag cataatctct ccacttcata
     9241 gccatctctc cattcttgta ttttagactt aaaaataatt atgtatttat gtacatattt
     9301 atttatttct ataaacacct acataaatcc tgttaaaagt gtaaattttt tattatgcaa
     9361 cttctctggt tatttctatt gattgatagt aaattattgt acctaaattt aatccaacta
     9421 acaagtaact aaacctactt tcctaactgc aaaatacact atgtttgcca cttgtatctc
     9481 atttgtaaat aaataaagtc cctacaaata cattaactag gctatgtact aaactgtagg
     9541 aactttaaaa atatattatt cttaattaag ttttggccaa taatttttat ttgcttctat
     9601 taaatcatct agtaattctt ttgcaactgt tgcactagga actgtcttag atagcataag
     9661 tgcttgccac aatttattat aagacttctc aatccaagca tcaactacta atttttcaac
     9721 tgctaattgt tgttctatta aacctttttg gaatgttggt attttcccta ttgataatgg
     9781 ttcatacccg tcttttccaa ctatacatgg aatttcaacc atagcatcat cttgtatatt
     9841 ttctatagct ccattatttt gaactatcaa taacatacgc tcctgtgtat taaaagctag
     9901 agctgtagct aaatccacta taaactctgc atgtattcct atcttaagcc cactgccctt
     9961 agaatgtcct tgcttttcta tcaatgcaca tgaaccaaaa acttcttttt ctcttccatc
    10021 aataacttca ttggcacgag tataatttac atcagaatga tttacaacat aatctggaaa
    10081 tagatagtac tttaagtaac tacttggtat tgtagttaaa tctgttttta ctatttcttt
    10141 agtaaataaa ttagtttcaa accaactatc tttcttgtca tttttcattc cttcttcacc
    10201 atttataaaa ccatattctt ttatatgttc tactaatttt ggtaaaagtt catttccttt
    10261 tttatcttta attgaagtat accaaccaaa gtggtttaat ccaaagtatc ttatatccat
    10321 atgtttacga gatttgagtc ctgcaatcct tgctatatta tgttccatac ctattggcat
    10381 atcacaaata tttattaccc tagaattagg tctcaacctt ctaacagctt ccgctattat
    10441 tgatgctgga ttagaataat ttaacatcca acaatttgga gaatactttt ccatataatc
    10501 tatgttttca agaacacctt ctatagacct cattccataa gctattccac ctggtccaca
    10561 agtttcttga ccaactaccc catgttttag aggtatcttt tcatctagtt ctctcatttc
    10621 aagttttcca actcttatat gagctaaaca aaaatctaca tctgtataag cctctttagg
    10681 gcatgttgtt gctaaatact ctatattagg gtccttttcc tttattagta tttcacaagc
    10741 tttagctacc ttattttgtc tttcttcatc attatcatat aatttgattg ctcttaatgg
    10801 taacctatct aagttatcta gcaacattaa tatgatttct ggggtatatg tacttcctcc
    10861 tccagctatt actactgaaa atttttccat aataatatct ccttttctat ttaaaaattt
    10921 actgtattta taaataaatt tataaatact agtactttaa agtaaaaggg agttcattta
    10981 ttaaatctat tgatttaata aactatagcc aaacttcaag cactatttac aagaaattta
    11041 attcttatta attcttgggt ccatcatctc aaatatatct acaagttcag atactaaaga
    11101 atgagcaaat aatgcatttg aataatggtc ttcagagtgc acaactaaaa aatcagtccc
    11161 actatcttca ctattcatag catttgatag taattctaaa tgatgctttg atgctgtttg
    11221 tattttctct gaggctgaat ttaaaaggtg ttttgcttta ttgtagtcac ctttttttat
    11281 ttccttaaga gacatcatta catcactttt agcatctcct gcttctgata ttatactaaa
    11341 tactttttct tccatttcag gtaccatatc cttctccttc taatgacttg ttaaaaatca
    11401 aagctatcat catctaatgt tgtagctaat tctgcatttt tatttaattc ttccttctcc
    11461 attatcttaa agaatggata atatatcaag gtcataagta ttatacttgc aaatactaat
    11521 acgattgcct tccaatctaa tgtcatcaaa aatgctgcaa cccctggagg taaaaagcct
    11581 ggtggttctg caataggtaa tcctactaat cctactttta atgccataaa attaagtgct
    11641 ccaaccaata caggtccaaa tacaaatggt acaaacatat atgggttcat aactattggt
    11701 gttccaaata ttaccggctc atttattcca aatagtgatg gtaaaatcac aacttttccc
    11761 atactattaa aactcttaca cttacttctt gacataaggt acactaatcc caaagttatc
    11821 cctgaaccag ttacttgcaa gaaaccatag taaaaaccta ttgtaaacac atattttggg
    11881 tctcctcctg ctgctattaa cgttgcattt tctgccatat atgtagctgc tattggcatc
    11941 catatacttg aagttattgc tgctccatga agcccaaaga accataataa ttgagttata
    12001 aatagtatta tagttactgc aaaaacatta tctacactgc ttatagctgg tgctaagaaa
    12061 ttcattataa tagctggtaa tggttgacct gagacatttt tagttattgc tgctattgct
    12121 gtaaataaca ttatacatac tatcattgga gttaagtttc taaaactttt atttactaca
    12181 ctaggtactt cttttggaaa tctaattacg agatttttat cattcataaa cttagtaata
    12241 tcaactacta aaagtgctac taatatactt acaaataaac cttgtgcatc taaataactt
    12301 gtatctaata caccatctac tacttgtcca catacaacaa gttgtgcaac aactgctgtc
    12361 atacctattt ctacatgatt aagattatat tttttagcta gtgcctgagc tataaaaaat
    12421 gctgaatata tagataataa actcatagta aatttattag ggataagtaa tgtatccata
    12481 ttttcagata ataatgtttt tgcacttcct tcaggtaaca tattgtttaa tgccataaat
    12541 aacatactta aagaaccaac aattattatt ggggtaatgc ttatcatacc ttcttttata
    12601 gcttgaagat gcctttgttt ttcaatcttt tgtgctattg gaagaagaaa tcttcttaaa
    12661 ttattttgaa gtgcttccat ttttattccc cccaattatc tatttttata taattcgatt
    12721 gccgacttca taacagaagg agcatccata cgaccataat gcttagtatc tattaaacca
    12781 actgctattt tgtaattctt acaagcttca actacatcat cttccttaaa cctaatttgt
    12841 ggtgctacaa gtactacatc ggtatcatta gcttcaatgt atgattttaa attttcaaat
    12901 gatgttgcct ctatatttat agttttagtt tttaactttt catgctcact acaaaaactt
    12961 ttcatctttt ccacaagtac acctgttgaa gctccatttg cacatactaa taaaatattc
    13021 ataattaccc tcctacaata tctaacctct tttattttct aattttttat cagccaattc
    13081 ttactctaat tcctatcagt ttttacttta attcttatta ttttatttta attcttatta
    13141 atatttactc taattcttag tactgccaat gattaaactt gaaagccgta tctataactt
    13201 cttcttgctt atatttttct aatattttta agtatctttc tttataatca ttatctactt
    13261 ctttttctgg tattactata ctagcttcat ttaaaaagtg gaatgaatcc cttcccattt
    13321 ctaatcctct atatgtatgc ttgtcaagtg ccacgtccat aacttctgga actttaccca
    13381 ttgcaaatcc ttttataatt atatttttta ataaatcact tgatctatct ttctcacatg
    13441 cacaaagata tcttattgca tgtatgaaaa ataaaggtct gtctccatct gcatatgaat
    13501 attcctttct tacttgattt aaattattta tgattattac agctgatggg tcccccattc
    13561 ctatatcttc aactgatatt gtaagtatcc ttctccatag cttatcttct aattctggtg
    13621 atgatatata tagttcataa gcaaattcac aagcttcttc caccatattt cttcttatag
    13681 ctttttgaaa tgacgatatt atttcatctg cactaaatcc gtttcttgta gtcattcttg
    13741 cccatgggtc atttgccttt ttatctctta atattttatc ttgcatatta tttactcctt
    13801 tttatttaaa ttttaattat ttatattact catttaataa tataagcaag tattatgcca
    13861 gtttttttag acactttaaa acactgttat ttcaatcatt tttatcatca aaacttagat
    13921 actttgtcta agtatattta tatgtgtcta atattaatat tattttagac taaaaaaaat
    13981 taccaaataa gtttttatac tcattggtaa tctttttcga ttatttctgc atattctaat
    14041 attaataata tttgatagca ctctttttca ttaatttcta tattatattt tttatctaat
    14101 tgagatacta tatcttttat tttgcttagg ctcttgtgat aatcaagtgt tttttcttta
    14161 tcaagctttt caatttctct agtatttcca atcaatccat ctaccatgaa agctaaatgc
    14221 atcataagtc caattacttt atcgtatttc ttttctatat taaatactaa aaacaattca
    14281 tcagacacat attttatatc tttaaatatc ctattataat tagagctttt taaggatgtc
    14341 tttaaacctt caactacatc atcataacct atacttatct ttataatatt ttttaattta
    14401 ttgatatttt catccttatc tatatctttt ttgctaatat agtcaattcc tctaatatta
    14461 attctttgtt cattgattac tgcaacaata ttactacttt ctttaagttt cgtaatattt
    14521 aaacttaaat ctttttctgt tttatatggg acagatataa tatctattcc atctaagtac
    14581 ctactcatct tttcttctaa tatttttctt gttttattat ctacaccttc tgaaacatgt
    14641 gatgctataa taattgtttt agacagattt tctttatttt gaatttcatt agttccaaaa
    14701 tagtactttc tcgtgttagt tatagagttg tatacgtcat ttaaaggtaa tcccatattg
    14761 gccttctgaa ctgcttctaa cacagttaat gttgtcacca taggaattac tcttactcca
    14821 cacttttttt ctttctttat aatttcatca aaagagttta atgaacccat atctgtaaat
    14881 agtaatacat ctttattgtc tatattatta attaaatttt taaaatctac aataacttca
    14941 tcatagcttt tatctaactt catatcgaaa gaaatagcac tgcactttcc aataatatca
    15001 ccaactgctt ttaccatact acttgctgta gtttctccat gcatagcaac tataatatta
    15061 atgttattca ctattttatt ttgttcatct tgtttagctt gtactatgaa taatgtaata
    15121 tatgctacag tttctataga taaaaataaa tcaaattcat tttcaattat tccaactatt
    15181 tttagtgctg ttttaaattc acttctgtta tttcttctaa tttgattcaa atctatgttg
    15241 tttgtagagc tacttttatc caatacgctt tttatataaa ttgccattga cttaaatatt
    15301 gattcactta tgtctatttc ttcatcttct ttaataattt gtcttattga ttcacacatg
    15361 cttttaaact taacattatt aaatagagaa gcatctgcta tattcatttc tacagattta
    15421 ttaagattag tatttactgc attaataata tttatatcat tgtagttaat aacttcttct
    15481 tctcctaatg acgtaaatac aaaatattct attccatgtg ttataacttc atctgcttta
    15541 atagacttct ctctataatc atcactcact tcatttatag atttatcaaa atattttttc
    15601 tctatataaa ctggttcttt attatctctt ttatatctta ggtatccatt tgcaacagct
    15661 aattttatat cattttgaag ttgacctaca ttatttttac aatcataaag taatagtgat
    15721 ttaattattt ctttatgtac ttttattgaa atagaaatac tttttgattc ttccttgaaa
    15781 aaatttttga ctaattccat tctttcaatc attgtcctat ctctcagtga tggtatttct
    15841 attttcattg gtattcttct attgaatgta tctaatagtg ttgatgtcgg attttctgta
    15901 gtagcacata ttattaatac cttagactct ctttctttcg ttgcatctcc tagtctttta
    15961 aatgttccct tatctataaa agtaaacaac atttcttgac cttctgctgg tagtctatga
    16021 atttcatcta aaaatagtat ccctccattt gcaatttcca aaagaccttt tctggaagat
    16081 tctgcacctg tataagctcc ttgttctacc ccaaaaatat gtcccataag taattgtgca
    16141 ttattagcat aatcagcaca attaaatgtt acaaaaggag ctttattttt tctaattcca
    16201 ctatctatag aataatcgta catatatcta gcaatcatac tctttcctac tcctgtttct
    16261 ccatagataa tagtatgaag tccatttgta ggatagagca ttgcgacctt gcatttttct
    16321 attataggtc ttaaactatg ttctttacct attagtgttt caaaactatc tttttttatt
    16381 ttgttttcta cttttgttcc tttttttatg ccaagcaact cactttgtgt atcaagcaat
    16441 tcattcttgt taaggttaag atttgcaggg atataataca caggtcttcc ttttatcttc
    16501 gtaaccatac cctcattgaa taattggttt aaatatgtac ttatattgtt tcttgcaatt
    16561 cctgtctttt ctgataattg gttagctgta atcccttttt cattttccct atatgatgat
    16621 tctaaaactt tcattacttt atctttttta tatatcatta caagctcccc tacattatag
    16681 tattttatta actaaaaccc ctatacacac tttatttttt aataaactct tattctttaa
    16741 ctaaggttat aatttatcaa actttcctta ctttaaatta tagaaaatta ttgtagaaaa
    16801 aataagatta ttgctcattt tttctacatt actattttta attttatagt actttcttaa
    16861 caacatactt tacttgtttt taattttatt atatttgaat ttattatatt tgaaaagtag
    16921 aaataaaaaa ccctattttg gagtagtttt aaaatttctc caaaaatagg gttttagttt
    16981 gtatttcaaa taaatacctg ttcaagtatt tttatactaa acaactttta aattttgttg
    17041 atatttattt atttctgatt tagaagaata tacaaaatgt ccatctgaaa catcaatcca
    17101 acttaatgac ttttcatttg aacaatcata tccattagaa ttatactcta ttctatttct
    17161 tgacttagca tatctagggt caggaacagg aattgcagaa agtagtgatt ttgtatatgg
    17221 atgaatagga ttattataca attcatttga atctgccaat tctactaatt ttccattata
    17281 cataactcct atacggtcac taatatgtct caccatagat aaatcatgag ctataaataa
    17341 acaagttaaa ttcttttctt gttgcaattt tttaaataaa tttactattt gtgcttgtat
    17401 agacacatcc aaagaagcta taggctcatc agctattata aattctgggt ctactgaaag
    17461 agctcgtgct atgccaatac gctgtctttg tccacctgaa aaatcagatg gatacttatt
    17521 tgcataactt ctatcaagtc ctactagctt tagcatctca tatactttat tcattctatc
    17581 tgttttattt ttacagatac cttgaatttt tagtggctct gaaattatat ctgcaattgt
    17641 catacgtgga tttaatgatg aagtagaatc ttgaaaaata atctgcatac ttttgtttac
    17701 atcttttttt ataaatttgt acgttttttt atctgatatg caatttcctt tgtaaattac
    17761 ttctccacca gtagattcat gtagtcttat tatagtttta ccagttgtag attttcctga
    17821 acccgactct cctaccaatc caaatatctc acccttatat atatcaaaac ttatatcatc
    17881 aactgctttt acagtagtac ttttgtccaa atgaaaatat tgctttaagt tttttatttc
    17941 cagtattttt tctctattca ttagaaatca cccttccaca atttactcta actggtacat
    18001 ctacttcagg tgcatctggg tgcaataacc aagttgctgc actatgtgta tcattaattt
    18061 taaacattgg tggctctttt tcataatcta tcttaagtgc atttttattc cttattgcaa
    18121 aagcatctcc ttttggtggg ttcaataaat ttggaggcat tccaggtata ttatataaat
    18181 agtcatcctg cgaatctaaa gtaggtaatg aacccaacaa cccccaagta tatggatgtc
    18241 tagggtcata aaatatatcc tctacagttc ctatttctac aatctttcct gcatacataa
    18301 ctgctattct atcagctata gttgcaacta ctcctaaatc atgagttata aatattattg
    18361 acaaacctat tttatgttgt aaatccttta ttaaatcaat tatttgagct tgtatagtta
    18421 catctaaagc agttgtaggt tcatcagcaa tcaatacatc tggattacaa gcaagtgcta
    18481 tagctattac tatacgttgt ctcattccac ctgaaaaatg atgtggaaat tgtttaaatc
    18541 ttttctcagg attatcaatt ccaaccaatt caataagctc tatggctctc ttttttgcct
    18601 cacttttact tataccttga tgaatgatta cagcttctgc aatttgcttt cctattgata
    18661 tagttggatt gagagatgtc atagggtctt gaaatatcat tgatatattc tttcctctta
    18721 tatcttccat ctctttttca gacttcttaa ccaaatcact tgatttaagt aaaacctctc
    18781 catttttaat atatccattg tatgggagta tcctcatcaa actcttacac agtacagatt
    18841 tcccacaacc agattctcca actatagcca atgtctcacc tttttttaga ttaaaagata
    18901 catttcttac agcctgaact tctccttctt ctactttaaa acttacagat agattattaa
    18961 cctccaataa atgttccaaa aaccatcaac ccctatgcta ttcaaacctt agggatatat
    19021 ttttatccct aaggtttcta attaatttca gtattattca attgtccagt cagctatgtt
    19081 ccagaatata cctacaccat gatgacctaa aactgtatca ggtgttaatc cttttatgtt
    19141 tggttttcca acatatattg catctatata agcaataaat gtataaggca tatccttagt
    19201 catttctact tggaattgtt tatataactt taatttttca tctttatctt ctgtttctct
    19261 tgctttttga agtattttat ctatagtagg attagagtaa gcactgtaat ttgcaccttt
    19321 atctgttcca aatactttat atgtatggtc atctgggtca aatggacttc cccaacctat
    19381 taaatgagca tcttgattag cccaatctgt ttcagttaca acaacagctt tagcatcaac
    19441 accaatttct tttaactgtt gtgcacatat tttagccata tcaactctaa cttggtcact
    19501 ctcacctgct gttatctcga aagctaactt agtaccttct ttttcataga ttccatcaga
    19561 acctaatttc caacctagct tttcaatttc ttgtttagct ttttctggat tatattcaaa
    19621 cttttctata tcaggattat tatatggtcc catttgtaaa ggtgaatatg ctggtacacc
    19681 atgtcctaat aatacactgt caactattgc ttttctatct attgcatagc ttaaagcatt
    19741 tggtaatcct ttagcttttt tatctttaaa gaatttagaa ttaaaattgt ataatatacc
    19801 tctatagtcg gctgttttca ttatatttac tttaaaattc ttttcgtctt tttcaaagtt
    19861 agacatatct ttaggagtta tttgagctag gtctaattct cctgatttta attgcattgc
    19921 cttagcctta tcatctggaa caatcttaaa gactacttta tctaatcctg gttcttttac
    19981 aaaataatca ctattcttaa caagtgttat actttgacct ttgtcccatt tttcaagttt
    20041 aaatggtcca gtacctattg gtttttgatt aaattcatca gtagctatat ccttaccttc
    20101 caaagcatgt tttggtaata ctcctactgt taaataatca agcattgctg tatttggagc
    20161 ttttaaagta atttttatag tattatcatt aaccacatca atttttgtta tatcttcata
    20221 atttgatgct atctcagaag cattatctgg attcattata gtttctattg taaacttaac
    20281 atcatttgct gtaaattttt ctccatcatg ccacttaaca tcatctctta aattaaaagt
    20341 atatgtattt gttgcctcat caaatttcca gtcctttgct agacaaggaa ccaccttatt
    20401 attttcatca tgagctgtta atccattaaa tattaacgaa tttatttctc catgttcata
    20461 tagtgcagga tttatacttg tatagtcatt acttccataa acaagcacct ttccatcttt
    20521 aggcgtatct gccttcttat ctttgtctcc tccactagaa caacctgcca ttagactaag
    20581 tatcattact aaacttaaaa cttttaactt ctttaatttc atgatgctcc cccttttgct
    20641 ttttataaaa tatttttcaa agctttgacc ccatttattt aaatagatat ttcaaatacc
    20701 tcagtatttg cttatttaat actcaaatgc tgatttataa attactagat tttttattat
    20761 tattctttct tatataatta cctatatttg ttatacacac caatgtggta accaaaaata
    20821 tacccggaat cagtataatc caccatctat ttgaaagtaa tgcttcctct gacaaagata
    20881 acatacttcc ccaagaaaca atttctattg gtaaaccaat tcctaaaaag ctaagtgtcg
    20941 attcagtacc aatagctgcg cctatgtttg ttacaaccat gaacattatt gatgccacaa
    21001 aatttggaag taaatgttgc tttaatacat agaagaatcc tcctcccatg ctcttagctg
    21061 ctagtatgta ttcgctattt tttatttgtc taacttctgt tctcactatc ttagatatat
    21121 tcatccaact tgttatccct attactatgg acatagatac tggatttgaa tttccaagta
    21181 tagcttgtac aaatattata attaatattg aaggtatact aagtatgatt tctgtaaacc
    21241 tcatcattgc atcatctaca agttcagatg ctgaaccact tatactacca taaacaattc
    21301 ctataacagt tgatattaca gtggaaaata gtcctataaa taaagatatt tttcctccat
    21361 accatatcat agaatatata tctctaccca ttgagtctgt tccaaataaa aaagttttat
    21421 ttggagctaa attagaactt gctaaatcca tgtatgttgg ttcatgtgtc attataaaag
    21481 atgagaatac acttccaatg actatgattg ataaaataat aattgatata tatggtaatt
    21541 ctttaatttt tttagataag gttttctttg tttctatatg ttcatcttct gctactaaaa
    21601 tataattttc tcctacaatt tcaaaatcac tatattgtgg cttcattagt atcacctcta
    21661 tcatatttca ttctagggtc tattttattg tttataattt gagctaacat attcccaaat
    21721 actactaaag cccctgttat taagcataaa actaataaca tattataatc atgatacttt
    21781 gcactttcaa aacaaagtgt tcctaaccca ggataagaaa aaactttttc aactacataa
    21841 gttcctccta gtatatgagg tatcgaaatg gccattatac taatatatga tggcattata
    21901 tttctcaggc aatgcttaaa aacaatagtt ctattattta aaccttttgc tttacataaa
    21961 agcacataat cttctcttat ttcctctagt aatttatttc ttatcatata tgtgtaatac
    22021 catagatggc ttaatataag tactgtaaga ggtaagatta gatgtgatat cctacttaat
    22081 atactggact cttctcccat tgaataagca ccactacttg gaagtatact taagtttata
    22141 ctaaatataa gtatgagtac taaggcaacc caaaatgatg gaatacagtt ggttattgtt
    22201 cctaatttgc atatgattct atcaatgagc ttatcttcat gtaatgtaca aaatattcct
    22261 aacaataagg ctagcacaaa ggtaagtata tatcctgaac cacctaatat tatagtgttt
    22321 atatagacat catttataac agaagtaaca ttttgtttat atttaaatga aattccaaat
    22381 tccccttttg atgcatttga aatccatttt atatattgac tgtatattgg ttcatccaaa
    22441 ccaagttttt tcatagcatt ttctttttct tgtgtactca ttctttctac tccatcccca
    22501 tagtaagaga ttaatgggtc tcctggagca agtcttgcca tgtaaaaaac aataaaagat
    22561 aataaaaaca ttactaaaat aaactgcaac aactttttca gtatatcctt taacaaaccc
    22621 caacccccct ttgttgcaat aaaaaaaacc acataaaagc aaacgtaaag taacgttaaa
    22681 accttcgtgg ttccattatc attaaacaaa attaaatttt ctatatttat ctatgagtta
    22741 attttagcat catttattaa ctgtgtaaac aaacttttta aaaattgtta aaatttcatc
    22801 aaattatttt atttatacct aaaataaaat ttttatattc tattcttcat tattttctat
    22861 aaaattataa aattttaggt tgactatata agaaaagtat attatattag tttgtatgca
    22921 aaccatttaa gaggtgattt tattgaaaag taattatgac tgttttagaa tagccatgct
    22981 cttaaaagaa ctttattcta aaactatgta cacagtagaa gaaaatttta aagaaaatgg
    23041 attaacacac caacagatta tagttataaa actgatagct cataatcaag aacttacaat
    23101 atcacaacta tgtgatgaaa tgtctttagc taaaggtacg gtttcaggca taatcagcag
    23161 attagaacag attggatata tagaaaaatt caagaaatct aatgataaaa gaaatacata
    23221 cgttaagttt acaacaacag gatttgaatt tgccacaaat tttaaaatta aaatgcaaga
    23281 aagttttgat gatattttta aaaattgtga tgaaaacgaa ttgagtgact tagtaaaaaa
    23341 tcttagaaat atattggcaa aagtaaagga gagataatta tgaattataa attaatactt
    23401 gcaatagtat ttataacatc agctttaatt ttctacacta taggtgtatt tggagaaaga
    23461 aaggcaaaaa ttttaaaaaa gaaacatgta attatatttt ggcttggttt tatatttgat
    23521 actttaggaa catttacaat gagtaacata gccaacagcc atacttttga agtaaaatct
    23581 gctttatcac aaaaccttca tagtattaca ggtcttcttg caattgtgct tatgcttttt
    23641 catgcaagtt gggctacttt tgtcctatac aaagatgatg aggaaaagaa aaaattcttt
    23701 cataagttta gtatagttgt atggactata tggttagttc catattttat aggtatgttt
    23761 attggtatgg ctggatagta aaataaaaag tgtgtaagaa atactgtagg taaatttgta
    23821 taagacaaat ttatctacat ccttctttca cacttttaca tatttattta taatactcca
    23881 ttatttcttt catgtttctt aatacctata tagaatattt aattgtttct atagtatttt
    23941 attattttct cacacttttt catatatata ttgttttact atttcttcat atgtatatat
    24001 gaaatattcc attatttcta taaatgtttt attatctttc tcatactttt tcatatatat
    24061 attgcttcac tattttttct tatttcttca tatacatatg aaatatttca ttatttctat
    24121 aatgttttat tatctttctc atactttttc atatatattg cttcactatt ttttttactt
    24181 ctttattaaa tacttatgta taacatacat ttaataaacg ctaactatta aacacctaat
    24241 acttgagtta ctcctggaac ttcactcacc aaaacatttt caataatagc tttgattgtc
    24301 atagtcactc ctggacaccc actacatgcc ccttgtagtc taactaaaac cacaccattt
    24361 tcatttacat ctataagctc tacatctcca ccatctcttt gtaatactgg ttttatcttt
    24421 tcctcaagca ctttttcaac tttttctctc atagctattc cccccagaaa tattttatta
    24481 gcaaaattat accaatggtt taaatttttg tccatctaat gatttatcaa attcaatttt
    24541 tgcttcatca ataattttag tatccataaa tacatcataa gcactacaag ccagtacttt
    24601 agctgaattt atcatagcag aaaatcctat atttgaacct gctgatgaac atgactgcca
    24661 tgtatgactt gcaattccaa caggccaagc tgccattgca aattgagcca ctggtattac
    24721 ataacttaca tctccaacat cagtagaacc agctagtcct ttatctttgt caccgtcaac
    24781 tatttccttg tgtatatagc tttctacaat agatttatca ttttgtaatg atgttgctac
    24841 acctaatctt tcttctctag taagagtatc acacaacttt tttgcaaatt cttcttcttc
    24901 aggagtattt ttagggcaac caacaaaaac catattgtca taaactaaat cagtcaaaca
    24961 ttggtttggt atatagtcat atattccatc tgtaacctta tactccattt cagtttctgt
    25021 catcattgct gctccttgag ctacttttat taatctatca agaacgtgtt ctgcatcctt
    25081 tgctttttta cctctaataa agtaccaact ttcagcaaat ccaggaacta cattaggtct
    25141 tccaccacca ttagttataa catagtgcat tcttatagag tcaataacat gttctctcaa
    25201 ataattagct ccaacattca taatttcaac agcatcaagt gcgcttctac cattatgagg
    25261 agcttgagct gcatgggctg ttatcccttt aaatttaaat tttacagata agtttgccag
    25321 acttccacct ctccaaggtg catttatatc aaaaggatgc catgtaaatg cacaatctac
    25381 atcatcaaaa cagccattta tacacataac tgtctttcct ccaccttctt cttcagcagg
    25441 acaaccaaaa tattttattg ttccattaaa tttattttct tctattaatt tttttataga
    25501 aacaactgct cctacacctg ctacacccaa caggttgtgc ccacatccat gtccagcttt
    25561 tccttctacc aatggctttc tttctgcact tacacattgt gatagctctg gtaaagcatc
    25621 atattctcca agtatgccaa tcacaggttt accctctcca aaacttgcta taaatccagt
    25681 aggtaaattt tctacttctt caattttaaa tccttttgac tttaggtatt ctttttgaat
    25741 gctacttgca tacttttctt caaaggctaa ttctggattt tcccatattt tcttagataa
    25801 atcaattaat tcttctctta tttcttcaac attatttatg atatactctt tcataatttt
    25861 cctccaaata tatgtatttt tgatatgctt tacagattat attatatact aacattctat
    25921 ttattcacaa ttttttgtta attaatttta ttcatatgct cttctatgga gtatatctta
    25981 tcttcacttt gcctcttcat aaatttaatg tactctatct tccataaagt agtttcagtt
    26041 ttctcattta acataaattc taatatatta atttctctca ttctaatatc ttcataaaat
    26101 actacactac ccaatattgc caattctaat tcacttaatg atgcaaacaa cattaaatac
    26161 tcttctccaa ataaacattc agattcaaga atatcgataa agtctttttt agcattgata
    26221 ttttccttta ttattcttat tttttcaatt atagatgtac tatctaatat ctcattgaat
    26281 atctctctaa acgattcatc atccaattta ctttcatcat caacaatata atcttctaag
    26341 tgttcctttt tatttttact cacagttacc atacttttaa aagtgttact ttttattgag
    26401 cttagcacag attcaataaa tatcttttca tattttttaa tatatcttat tacatcttca
    26461 tcttctatgc ttaatctttt tattaatact tcaattgctt ccataagtag taaagttatt
    26521 tttttattta ttctaatttc tctgttaatt tcttcaaaat cattataatc caaattacta
    26581 tctacttcag tgttaaaact atcatctact tcaatcaatt caattatatt actttcttca
    26641 gagtctataa ctttatcttc agtaatatta ttgtacatat ttttaaattg tttttctaaa
    26701 aattcatact catattttga aatttctaaa ttagtcactt catttcctaa aatagtagaa
    26761 aaaatacaat tattcataat aagttcaaat aaatttacaa gtataacatc acaatctatt
    26821 ttataagtca tcccatagtc ataaaataat ttttctctat cttttttctt aaataggtta
    26881 caaaatctat tttctatatc tatactctca atataattct tcacataata aataccctta
    26941 acatccatat catctagtgc caatggataa tctatactta ctggaacgtc ctgagcttca
    27001 aattttacaa tataactttt aaagaaactt gataactcca tcaaggtatc attatatgct
    27061 attgaattag tatccaattt tctactcatc attagtttat aaaaatcttt tagcctaact
    27121 aactcttcct ctaatatttc aatgcctcca aggtacactt ttttaacatt cttagcagtc
    27181 aactccacaa ctcttttttc tatatctcca agcttattta tatatgcatc aatagcatac
    27241 catattgaaa tcaacagttt ttcggcaact tctgttttta ctgatgtgct ttgaccattt
    27301 gtatattttt taatcaagtc cataagtatt agagatactt cttgttgtat attatatact
    27361 tctctttcat ctatcacttt acaatttaga cattccttaa gtagagatat ggaatattgg
    27421 ttctttatta aattattttc gtttatatta atattactta aattatttaa catacaaaat
    27481 ctctcctatc atatattaaa cttctccaaa tttaaatttc tttgcaaatt cgtctgcttc
    27541 tcctcttaca ttgtccatat tgcctgaaaa tttttcataa aattcaccca gtatttctat
    27601 aatctcatca tcacatatct tgtcatcagt ttcatttttt aaataataaa aaatttcttg
    27661 catatcatta atagcctcta aataattgtc cacatttgta tattgtgatg tatatatatt
    27721 ttctattaac tgttttgtaa cttttatatc taattctatt cttccatacc cctttaaagt
    27781 attatctctc gaatttatta tatcttttac atcagattca gaaagagcta gaccataatc
    27841 tccagattct atattagttt caagcagttc atttctttct atttccttta aattctgatt
    27901 tatagaactc aaaataatag gtgatatcat ttttaaattc tcctttttta ttttttccat
    27961 tgactttttt tgttttttat gctatactta aattaatgta tattatttta ccaaccactt
    28021 ataatagtag tgttaaaagt ggtttttgtc aactcaaaat tactatctca attaaacttt
    28081 tcaattttct caacaaaaaa ataccatcat ttagatatat aattttctat aacaactttt
    28141 aattcagtag ttatttacta aaatctaaac aatgatatat ttattaattt tatttatctt
    28201 ttttggctct acttctactt ttctttacat ttgagcaatt tctacaagaa ggtgaacagt
    28261 attgagattt agaatttgta ggaatataaa ttttaccaca gtacttgcat ttatttaaag
    28321 aagaaggttc acttgtaatt gcaaaggcat agatagtctc aatagctgtt ttcaaagaat
    28381 caaactccca tgcaataatt ggtttatcta attggcttat agaaaatcct atcttttgag
    28441 gattaaaccc tccagacata atagtaacac tttcagttaa tagattagat gtatttttat
    28501 atacacaaat ttgattaaaa tgcttacaca tatctttagc aaaacttaca atccaatcta
    28561 gttgttcagt gtagaacttt gaaaatacta aatctaaaat caatggtctc tttccaaaaa
    28621 actttggaga ttcttctctc tttgatacat ctatacccct tctatacttt ttaaaataca
    28681 catcttcatc acttgcaaat ggtataaaca tgtttacata ttcatctgta ctcaatatat
    28741 tctcttttgt aatataattc ttttcaatca tcagtactct ttcttctcca actatatctc
    28801 tattatatac acttgaatat attaaaccca ataatccata cttttttaca aattcaagta
    28861 cttctttctt tagtttcatt tcttttgtct cttttgatat atcttgtaat gcctcttgtc
    28921 ctatgcgaat tatatctact attatatcat ctgctacatc aaatgggtta tacatggtaa
    28981 acacagcatc ttctgttgga actatatata attcatcttc ttcagtaatt ttatattcat
    29041 aatcactata cctaatccaa tcacttgcaa aattccctat taattctatt tttttattca
    29101 taataatcat tcacaccttt caatgtaata tataacttca tattactatt acacttttag
    29161 tttattccat attttctaca ctgtcaacta ttctttttat atttttagca tattaaaatt
    29221 tataatagcc tttataatgg atttttacag ctaaatttct tgtttattga tgactaattc
    29281 ataattaaat ttataaaaac tatttacata taaaattaat ctctttataa ttgatatgta
    29341 atatattttt acacttacat attacatatc aattataaag agaggtatct aaaatcatgg
    29401 atagaaaacg tgaattaaaa caattataca aagaaatgaa atttgacaca ggtgtattca
    29461 taataaaaaa tgatattact aaaaagattt accttggaaa aagtaatgac ataaaaagca
    29521 aatttaactc tctcaaattt cagcttggtg ctggctcatg tatgataaaa gaattaaata
    29581 atgaatggaa aaaatatggt gaaaaagcat ttacatttga agttttagaa cttataaaac
    29641 atgatgataa caaaacagag aaagactatt tagaagaact agatatttta gagatggttt
    29701 ggttagaaaa attaaaggaa gataatactt atgaaattta ttccatataa aaaataatat
    29761 aatataagaa aagtagatag tgtctcaaaa tagacattta gttcataaga tactttttta
    29821 tataaaataa agtatctttt cataatttta acaatgaaaa agaggcttat ctccatttga
    29881 tagcctcttc ccatatattt tataccatta taaaatgaac aatctactaa ttttcaatgt
    29941 atatattcat tttactaata acactccatt acttctttac agcatccaaa cattgctcta
    30001 taatactctt atcacttata cctattgctg ttactttact agctccactt aatatactct
    30061 tatcactttt atttgaaact aaaacaacag gagcattttt tgcaagtggt gatgcaacta
    30121 aagcataaac taaatcatct ccacttgtta tatagaactc ttttgctcca ttatagaact
    30181 tttcaataac atttttatta gtttgatatc tatcaacacc accaattcta gttgctctat
    30241 atctgctcac tatgtaatca cttaatgtag actctcctcc aatagcatat actggaacag
    30301 gagaaactcc aaaccatggg tctaagtctg tgtcttcttc tgctgaaggt atatcttttg
    30361 tcaatattat aggagctcca tctctataag caacagatgc aacacttact gcatcagctt
    30421 catccttaaa tccatttact aaaaacatct tatcactgtt tttaattttt tctatttcac
    30481 ctgcaatttt ataacttgta tctactctat ctacaccatc aattcttata acttgcattc
    30541 ttctcataag taacattttt tctacatctt tacttataga attttctcca cctattacat
    30601 atatcttagt aactctattc agtctttgaa gtgttgcttc tggtatatca tccttattag
    30661 ttaataaaat agctgcatta tttactcctg caagaccact tgcagcaatc ccatctacca
    30721 ttgtagagtc tgcattgata agaactgctg ttgtaaaatc ttgcttatct gaaataagcc
    30781 cagctgtttc atatttatct actccttgaa tattttcaat cttatctagt gcgtttattg
    30841 atgttggaaa actaacactt aacagtaatg ctgaaatacc taacgccaat acctttttac
    30901 taatcctcat aaattacctc cctaaaacaa atatatttag tattagtata atataaaatc
    30961 atttaaaaat agtgtaaaaa tagttacact tttttataat ttaaataatt ttagactaaa
    31021 cacaattcat ccattaaaat aacttcattt attctatatg ctaagccatc ccacgttttt
    31081 tttgacatat ctacagatgt tttcttattt gagttattca ataaagattg tatcttagaa
    31141 tctattgcat tatacaaatt attttcaaaa tcatataact cttcatcttt tggaatacct
    31201 tctttctcca taaaaggtag agaaacatat tctatctttc cagaattatt aatctcacta
    31261 ccaatccact ctttaacacc aagaatatca gtagtgatta catctgtgcc acaagataaa
    31321 gcttctaaaa ctacaactgg taatccctca taaaatgatg gtaacacaaa tatttgtgac
    31381 ctgttgaata gctcagctaa atctctctgt tctaactttc ctaggaaatt cattttaaat
    31441 ggagacttac ttgccagttt aactatctca ttatatgatt cgatatcgct tccagtacct
    31501 gcaaaattga cttctattaa atctttacta tattttaatt tagatatagc ttttataaat
    31561 ggaatcagtc cctttgattt acatatctta cctgcaaata ctatttttat tttatcatcc
    31621 tttattttat aatttttatt ataaaatata tcatcattat aaccactacc aattacaact
    31681 actttacttt cacttactcc aaaagtcttt ataatatcat atttctgatt ttcatgaaga
    31741 gcaaatatta aatctaattt ccttatattt gctattatgt attctttctc taaatcaatg
    31801 gtatttaact gtcttaaaca agttccatga caaatagaca taacctttat atcttttacc
    31861 atttccctaa caaatgcagt taagaggtac aaatgatgac atattataag ttctggttta
    31921 aaatcattca tagccttatc tatatttact ttaaattgat atttcaatct atttatcatg
    31981 tctatattta aatctttata tcttgtactt tcatatggca tagaatcact catacctaca
    32041 acaggaaaat ttaactcccc acaattatac tttactggat aaaaacttac ttcacttgga
    32101 aaacaattta catcatcatt tacatctata cctgcaatta cagcttgctt atgaccgatt
    32161 ttttcgaacc cttttatcat accagacaaa tatataccac tacctgtact atttggtttt
    32221 tgtgttatta tatgaagaat tttcactttt cacttgcctc ctttctaaaa ttaaatagtt
    32281 ttatacagtc taatcattat atatatctaa atataaacaa gatgaatcat cttttacttt
    32341 aaatcttggc actttaaccg ctttatagtc attatcttca aaatccctta ctttattatg
    32401 tatatatcct ataccaaatt tttctgctat acgtatcaat tcttcttctt ttattagatt
    32461 atatctatca catgcacttg taaaaccatc actcgttagc atcaattgaa aatctttttt
    32521 tatttcaatg tatccatata tagaattttc cacagcttcc tcatcaaaat ccaatatcca
    32581 atagccctct tttgtatttt tcttcaatct attgcttact attgtatcca taacactagt
    32641 tctaatctca tcaaaagcca tctcttccaa gttagaaagt ttactcattt catcaaatac
    32701 agtattatca aatttacaaa tactcctatc ttttataact ttggaatctc catttttaat
    32761 aaacaaagca caatctccta ataaaaaata ctctacttta ttttcatgaa attttacaac
    32821 aactatagaa ctagatggta aatctaattt ttcaacctta aacccattta atcttagttc
    32881 atactctttt tttacttcta ttattccagt attaataata ctctttaatg attcttcttt
    32941 atttatattt ttatataaat acctattcca ccactgaaca taccaccttg cgtcactttc
    33001 agttgacact aaattttttc cattaagtcc tgtagcacca tctaaaaccc atgccccaaa
    33061 tggatttata ccgactacat cttcattaaa tttagctgtt ccttgattac ataataggct
    33121 atagtttctt atatttaaag ccatatcaac ctccaaattg aatatctaaa aataaagttc
    33181 tatttaaaat tatacagcaa ataaaaaaat ctacaacaaa acttgttgta gatttttact
    33241 taatctatat tatctttata ctttcagtat tttaatgatg gaataatcta tttttagtaa
    33301 atgccatgac tatgttatac ttattacaag ttaatataac attatcatca cgaatagaac
    33361 cacctggttg tgctataaaa cttaccccac ttctttttgc acgttctata ttatctccaa
    33421 atgggaaaaa tgcatctgaa ccaagtgcta ctcctgttaa agttttaagc catgctcttt
    33481 ttccttctcc agttagaggc tctggttttt cttcaaaaaa gttttgccat atgccatcag
    33541 caagcacatc catataatca tcagaaagat atacatcaat agtgttatct ctatctggac
    33601 gacctatatc ctttttaaat tttaagttta atactttagg atgttgacga agataccaag
    33661 tatcagcttt gttacctgca agtctagtac aatgaatacg tgattgttga cctgcaccta
    33721 cccctattac ttgtccatct ttagcataac aaacagaatt tgattgtgta tattttaatg
    33781 taataagagc tataattaaa tcacgttttg cactatctgt aaaaatttta ttatcagttg
    33841 gaatatcttt taataggtct tcatttatta atatttcatt tctaccttgt tcaaaagtaa
    33901 ttccataaac atcctttgtt tcaataggtt caggagtata attttcatct atttttacaa
    33961 tattatagtt acctttacgc tttcctttta atatttcaag ggcttcatct gtatatcctg
    34021 gagctattat accatcagaa acctctcttg ctataatagt tgcagtctcc ttatcacata
    34081 catctgaaag tgctataaaa tcgccataag aagacattct atctgctcct cttgccattg
    34141 cataagcaca agctaatgga gaaagctcta aatcatcaac aaaatatatt tgctttaaag
    34201 tatctgataa aggtacccct actgctgctc ctgctggact tacatgttta aatgaagttg
    34261 ctgctggaag acctgtagct tcttttaatt ccttcacaag ttgccaacta ttaaatgcat
    34321 ctaaaaaatt tatgtatcca ggttttccat ttaatacctc tataggaagt tctccattct
    34381 tcatataaat ctttgatggt ttttgatttg gattacagcc atactttaat tctagttctc
    34441 ttgccatata ctcatccctc tctataataa attaatcata agtttgatta atcctatatt
    34501 aattaaatta ttcgtactaa taaaattata tggcatattt atccattaat caactatatt
    34561 ttttaaaact cctattcctt cgactttaca ttcaactata tcgccatgct ttaaatatac
    34621 ttgaggattc atccccatag caacccctgc tggagttcca gttgaaatta tatctcctgg
    34681 ctttagagtc attcctcttg ataaatcatt tattatagta ggtatatcaa atataaagta
    34741 ctctgtatta gaatcttgtc ttacttctcc atttactaca ctacttatat ctaattttaa
    34801 aggatgttca aattcttctt tatgtactat acaaggtccc atagatgtat gtgtgtctaa
    34861 gctttttcct ctaaaccatt gaacatgttt atcttgaagt gctctttcac ttatatcatt
    34921 tactattgta tatccaaata tatattcata agcatcttct tttgatatat ttataccttc
    34981 tttacctatg attactgcaa gttctgattc atagtccaac ttctctacca aatctccatg
    35041 taagcttatc ttatcatcca ctccaattat tttgtctgct ctcttagaaa aataaattgg
    35101 ataatctggc ataactacgt tttttataac tccatctggg atttcattga catggtcttt
    35161 atagtttaga cctaaacata taatatcttt tttaggtctt acaattgggg aacataattt
    35221 aacttcttct aacttataac actttgcgtt ttcaaatgaa ttgtttttta ttttttctaa
    35281 atctcctgta gatacgtttt caataaaatc attcatatca gtatataatt tacttaatcc
    35341 taaactatta atttcatata tactatttgt ttctgaatta aatacaccta ttttttcttc
    35401 attgctacta caaaaagtca caaatttcat ttttattcct ccattcaata agttgattaa
    35461 tattatttaa tttttattag tt
//
LOCUS       DAYATN010000026        34849 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.026, whole genome shotgun sequence.
ACCESSION   DAYATN010000026 DAYATN010000000
VERSION     DAYATN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.026"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(332..1213)
                     /gene="pepI"
                     /locus_tag="ACGI8A_001256"
     CDS             complement(332..1213)
                     /gene="pepI"
                     /locus_tag="ACGI8A_001256"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase"
                     /protein_id="HFH0179259.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1669..2643
                     /locus_tag="ACGI8A_001257"
     CDS             1669..2643
                     /locus_tag="ACGI8A_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFH0179260.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(2759..3820)
                     /locus_tag="ACGI8A_001258"
     CDS             complement(2759..3820)
                     /locus_tag="ACGI8A_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFH0179261.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(4432..5283)
                     /locus_tag="ACGI8A_001259"
     CDS             complement(4432..5283)
                     /locus_tag="ACGI8A_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0179262.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(5300..6220)
                     /locus_tag="ACGI8A_001260"
     CDS             complement(5300..6220)
                     /locus_tag="ACGI8A_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFH0179263.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIKGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(6330..7244)
                     /gene="murQ"
                     /locus_tag="ACGI8A_001261"
     CDS             complement(6330..7244)
                     /gene="murQ"
                     /locus_tag="ACGI8A_001261"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFH0179264.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(7343..8440)
                     /locus_tag="ACGI8A_001262"
     CDS             complement(7343..8440)
                     /locus_tag="ACGI8A_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFH0179265.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(8673..10019)
                     /locus_tag="ACGI8A_001263"
     CDS             complement(8673..10019)
                     /locus_tag="ACGI8A_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFH0179266.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(10108..10416)
                     /locus_tag="ACGI8A_001264"
     CDS             complement(10108..10416)
                     /locus_tag="ACGI8A_001264"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0179267.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(10679..11077)
                     /locus_tag="ACGI8A_001265"
     CDS             complement(10679..11077)
                     /locus_tag="ACGI8A_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179268.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(11079..12230)
                     /gene="anmK"
                     /locus_tag="ACGI8A_001266"
     CDS             complement(11079..12230)
                     /gene="anmK"
                     /locus_tag="ACGI8A_001266"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase AnmK"
                     /protein_id="HFH0179269.1"
                     /translation="MYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEIPKD
                     IKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHGQTI
                     FHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEFLLY
                     SDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYDKNG
                     YFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDLIAT
                     VTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQDEYG
                     YSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(12670..13689)
                     /locus_tag="ACGI8A_001267"
     CDS             complement(12670..13689)
                     /locus_tag="ACGI8A_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFH0179270.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLIKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(13683..14363)
                     /locus_tag="ACGI8A_001268"
     CDS             complement(13683..14363)
                     /locus_tag="ACGI8A_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0179271.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GVGYIIQC"
     gene            complement(14515..16422)
                     /locus_tag="ACGI8A_001269"
     CDS             complement(14515..16422)
                     /locus_tag="ACGI8A_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0179272.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAMFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(16423..17175)
                     /locus_tag="ACGI8A_001270"
     CDS             complement(16423..17175)
                     /locus_tag="ACGI8A_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179273.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(17491..18645)
                     /locus_tag="ACGI8A_001271"
     CDS             complement(17491..18645)
                     /locus_tag="ACGI8A_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0179274.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(18648..19115)
                     /locus_tag="ACGI8A_001272"
     CDS             complement(18648..19115)
                     /locus_tag="ACGI8A_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFH0179275.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(19129..19617)
                     /locus_tag="ACGI8A_001273"
     CDS             complement(19129..19617)
                     /locus_tag="ACGI8A_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179276.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(19631..20962)
                     /locus_tag="ACGI8A_001274"
     CDS             complement(19631..20962)
                     /locus_tag="ACGI8A_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFH0179277.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(20976..22541)
                     /locus_tag="ACGI8A_001275"
     CDS             complement(20976..22541)
                     /locus_tag="ACGI8A_001275"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucoside-specific PTS transporter subunit
                     IIBC"
                     /protein_id="HFH0179278.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(22711..23496)
                     /locus_tag="ACGI8A_001276"
     CDS             complement(22711..23496)
                     /locus_tag="ACGI8A_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0179279.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(23663..24862)
                     /locus_tag="ACGI8A_001277"
     CDS             complement(23663..24862)
                     /locus_tag="ACGI8A_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFH0179280.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(25384..26721)
                     /gene="xylA"
                     /locus_tag="ACGI8A_001278"
     CDS             complement(25384..26721)
                     /gene="xylA"
                     /locus_tag="ACGI8A_001278"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFH0179281.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(26817..28367)
                     /gene="xylB"
                     /locus_tag="ACGI8A_001279"
     CDS             complement(26817..28367)
                     /gene="xylB"
                     /locus_tag="ACGI8A_001279"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFH0179282.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVSSLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            28575..29735
                     /locus_tag="ACGI8A_001280"
     CDS             28575..29735
                     /locus_tag="ACGI8A_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFH0179283.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(29891..30295)
                     /locus_tag="ACGI8A_001281"
     CDS             complement(29891..30295)
                     /locus_tag="ACGI8A_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0179284.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNANLLSDDE"
     gene            complement(30316..30798)
                     /locus_tag="ACGI8A_001282"
     CDS             complement(30316..30798)
                     /locus_tag="ACGI8A_001282"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0179285.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(30818..31627)
                     /locus_tag="ACGI8A_001283"
     CDS             complement(30818..31627)
                     /locus_tag="ACGI8A_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFH0179286.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(31620..32399)
                     /locus_tag="ACGI8A_001284"
     CDS             complement(31620..32399)
                     /locus_tag="ACGI8A_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFH0179287.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(32399..34477)
                     /locus_tag="ACGI8A_001285"
     CDS             complement(32399..34477)
                     /locus_tag="ACGI8A_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFH0179288.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
BASE COUNT        11485 a         6136 c         3713 g        13515 t
ORIGIN      
        1 atctattcct atttatataa ttgtttctta ttataattaa tatacttttt aatcaaattt
       61 aaacagaata attataccaa tatataaaaa aatttctaaa aaacaataaa attaagttat
      121 aaacaccaat atatttctat tatttcaata agtatacaat tagattagaa tctaacgcta
      181 gtaataaaaa attactttcc caaaatagat ataagcctca ttttatcgtt caaataatga
      241 aaatatattt gatttcatgc aaaaagagtg cctaatgaac taaaaagttc attttgagac
      301 actcattttt ttatataaat ttatatagtt tttaatcgtt cgcattcaac cactcagtta
      361 agacttttat atatttctca ttttcttcaa cgaatggcat atgtcttgaa tactcaaata
      421 attcccactt actattaggt attctatcat acatagtctt tgcaatatat ggtgaacata
      481 aatctattgc tccactagtt actagacaag gctcttttat ttcatgtaat ctatctgtaa
      541 attcatatcc agaaagagtt cctgtaggag aaaattcatt ttgtccccat cctacaatat
      601 atgcttcaga acctgatttt ttaggtcttc tcaggcactc tggagaatct tctgtcactt
      661 cacctgcaca atacatttcc ataaatcttt ctaatgcatc attatattct ttgctagagt
      721 aatctccagt atttactgca tcaagtaatg ctttttggtc aacctcactc atatatgata
      781 ttcttctttt ttgttctttc tcccaaagtt ttgcagaaga aagagtagat gaaaggatgt
      841 aagacttaat accttttggt ttatattcaa tagcatacca aatagcctgc atacctcccc
      901 aagattgacc taacagatga atttcatcaa gtcctagatg ttttcttagt tcaataagct
      961 cctctatcca agtatctgca ttaaatagct caggatgccc ttctacaaat gaatttccac
     1021 aaccaatttg gtcatacatt attacttgtc taccactttc agctatctta tccaatacct
     1081 caaaatagtt gtgtgtagaa cctggacctc catgaagtaa tactaatggc ttcttccctt
     1141 ccgtattttc tcctactata cggtaatacg ttttaaaacc tttaaaaggc atatatcctt
     1201 cagtaatttt catgtttatc tcctccaatt tgtaattata aacaataatc aagttcatta
     1261 acaatactat ttaaaacctc ttatacatat ttgattatgt attttaatct ttatacctac
     1321 ttttataaat agtataacat aaaagttaaa aatctctata ctaaaccaat tataaaatta
     1381 tattaactat ataattttat tttttaataa tagtatatac atatttgatt attttcattt
     1441 gctatttaaa atcaatttta agttttcttc ttataataaa cttcctcttt gtggtctcat
     1501 gttagtctct aaatttaaaa ttttacaaaa aattttcata aaataaaatt atattgattg
     1561 tatctaaaca aatagggcaa taaaataatt aagtatagtt acttaccttt caagcaatat
     1621 tagttttatg ttagaatttt attatattga tttacaggag gtttgcattt gaataatatt
     1681 aattcactta aagtaataaa attatctctt ggatgttctc ttgctatatt tattgcttgg
     1741 ctattaaaat tagaatattc aatggttgct ggagttatag tactacttac agtaaaggac
     1801 actaaaagag aaactcttaa aggctccatt ggaaagattt atggttttct tttatgtact
     1861 attttttcat atttatgttt taatatttta ggatataatt tgacttcatt ttcaatattt
     1921 atatttataa ttataccact ttgttttttg ttaaatattc aagatgttat tgctatgtgt
     1981 gtagtcatag catcccatta ctaccttcaa ggagaaactt ctatcaaatg gattttaaat
     2041 gaagctggaa tatttgcagt aggaactgga ataggtgtct taataaatat gtatattcca
     2101 acaaatatac ataaaatcca tgaagttcaa aaaaagttac aggaagaagt gagtattgta
     2161 cttatagaca ttgcagatat tattgtaaat cctaaaaaag aaaatggata tagtagagat
     2221 ttatatacat taaattcact tattgacagc agtatttctg aaacttacga taatattaat
     2281 aatactttat taagtgatac tagattcttc ttagagcata tggatattat aaaaagtcaa
     2341 cgggatatct tagaaaattt gtatagttat gtatctcaac taaattctac accaccacaa
     2401 gctcatattc tatctgcttt tatacataag ataggttata ctgaatttga agctgaaaca
     2461 ggcaatcttc ttcttgaaga attaaagaga ctaatgctta gtatgaaaaa ccagcctcta
     2521 cctgttgaca gaacagagtt tgaaaataga gctattttat tcctatgctt gactgagtta
     2581 aaacagtttt tagttaatcg taaacatgct cagatgctta gagataataa tttttataaa
     2641 taaaaagggg agaaatctag cagttgacct gactacaaaa tatcagatat aatattaaat
     2701 gcgcccctag taagagggcg caatgtttat aacttattct actgtcatga ttaaatcatt
     2761 attcatatcg aacatacatt atttgaccaa gtccaatatt agctttttca tcagtgattt
     2821 ctattttaat agtcacatca ttttttttac agtacttcgc taattctgca ccagaacgat
     2881 ttttaccact aaaatctaaa gaaaatggct tatcatattt aaaaggatat tcaacaggtt
     2941 taccctctgg tgctaatgca aacatcccat atggtgtgtt cataaatcct attacagcaa
     3001 tcgtaagatt tgatgggtct aaatttgatt cactacctgt aaatgtaatt gtatttactg
     3061 atgaattaac tacactacat cgaacacctt catacattac atgtttttca tttatatctt
     3121 ctcctttttt cccaaaattt ccaacagcct ttgccccttc ttgatgtggt aacatagtgt
     3181 gcttcccagg tggtgctggt ttctgtgtct gttctggttt taccgtctgt actattgcac
     3241 caacttttgg atatgctgtc ttatcaaatg gtaatgggtg gctaagactc ttgtatatga
     3301 tatcctttcc tgctgattta ttagcatcaa tatctacatg atacaaacgc ccatcaccaa
     3361 aacggataag attccactgg tggtcatttg atgtgctgtg tacataataa gattcaattc
     3421 ccatagcatc acacaaagct ttcatgctcc tagccattcc tgagcatgca ccttttttag
     3481 taaccaatgt ataggcagta tttgctccct ctactgcatt ccagtcaaca tatgttgtgt
     3541 tatttttaat gtgattgtag gcaactaaaa tcttagcatc tgtggtcata ttctttgtga
     3601 ttttcttgtt cataaaatct gctgttgctt gtgctactga gtttagttgg gcttgtgaca
     3661 gctttggacc atatacgcta ttggttccag tgacatagct ttttggtgtt tttgctgtta
     3721 cctctttggc tgtcatacca taaactgtca tactcatacc tgagattaaa actattgttg
     3781 taatcaataa cttggtagct aacttcctaa ttttcttcat acttaatatt ctcctttata
     3841 aaacattgtt acttcctatt ctctaccatt aataaaatta atttaggatt taaaaaggtg
     3901 gattgtcaac atgtatattt ctccattcat acattcgtga aatatttaaa tttcatggaa
     3961 aatctaatag atattttcta gataatttgt atacaataca actcgcaaat cagggtgttt
     4021 gctgttagtt tgagaaaatg atgatgatta atacattcgg aatctttgac ctataataaa
     4081 taccattgtc ttcaatcatt aataatggta agagtaaaga gaattatcaa taatacagtt
     4141 aaaatcaact ttgtatacat ttcatacatt ctctcccaac aacattatac ttcttataat
     4201 ttgtatattc aatacgtttt ctgggaaaat ataggatata tctataaaaa tataaaaaag
     4261 gagttgaact taaaatttga ttaaaaaaaa tcaattttaa gacaactcat ttgtattata
     4321 aaaatgctat atattattat taagtaagtc aatattttag atatttatct atttcttatt
     4381 gacttacgct tatagtctaa cttatttgtt tagttgatat ataacctagt tttaagcatt
     4441 attaatattt aaataatcaa gtatcctttt tgtttcttgt agtttctgtt ctgcctcatt
     4501 taagttttct tgaattatag aaatatatat tatatctatc aacgtaagca ttgctattct
     4561 tgaagaaata gctccttctc taagcctttt ttctatattt ggtactacta gttttaaatc
     4621 actaatctta ctaagatgat tgtcactata tttagttata caaatgacct tacatccttt
     4681 taattttgca ttttctatag actttattac ttcttttgat tctccagagt aagatattgc
     4741 tatagctata tcttctttat ctacatttga agataccata agttgtgtat gactatctaa
     4801 agaggtaaat gcctgtttat ttatccttaa aagtttcatc tgtaaatcta gtgcaactaa
     4861 agccgaacca cctactccaa atatatatac atttttagcc tttattatct gttcaactgc
     4921 ttcttttatt atatcttttt cattcaacaa ccttgtttgg ttgatagtct ctatgttttt
     4981 gtttgtaatt ttgtttataa cactatctat attacaatca tagttttcat ctacatatgt
     5041 gtattcattt tctttgccca tcaaatcttt agctatctca atttttaaat ccccaaaacc
     5101 cgaatatccc atttttctag aaaacctaac tatacttgct gtacttgttt ttgagtttga
     5161 cgccaattct gatacagaaa gcttacttat agcctctgta ttattaagta tgtaatcagc
     5221 tattttttta tctacttccg ttaaatcttc atatatctgt tttaatttta aaatgcaact
     5281 catactctat cacctctacc tatttactat aatatttctt cttagcaagt aaacatgctc
     5341 ctaaatctgg agtcaacact ggtctcacta taccaaatct cgttttattc aaatattttt
     5401 taagtggctc taatattaaa tctcctgact taaacactcc acctgaataa gataccttta
     5461 tctttgtacc ttgttcaaaa tgatgctcta atcctattat aagcctactt aattcataag
     5521 ctgcatcatc aaatatttta ctagcagtat tatcaccttc aataactgct tttaaacata
     5581 tcgtagcaaa ctttgctatt tctattctat ctccttttat ttcatcattt acatatttta
     5641 ttatgtcata atcattctct atattaagat gtctttttat taaatcatat aattttgttt
     5701 ttgaacacct tccatcagac tgcattgtga acatttttaa ggtagccatt cctatataat
     5761 acgctgaacc ttcatctcct aatacatagc cccatcctcc acatctgtga agattcccat
     5821 ctttatctaa agcctgtgct attgaaccag tacctgcaat tatatttata cctttttccc
     5881 catttaaaga acctgctaat gctatttcta catcattacc tagagtataa tttatatgac
     5941 tatatgcatt ttttgttgct acttctaatg catacaatac ttcttttatt tttccatatc
     6001 cagcaagtcc aacaaatgca tacgttattt cttctacatt tatcttagca tctttacaaa
     6061 cttcttccaa cccagttatt aataatttcg ttagattatc aaatcctatt tgattataat
     6121 gacaagttcc tttggttatg gttcctacta tatttaattc ttcatctact aatgtaaatg
     6181 ttgttttcgt tcctccacca tctacaccta aaaaatacat actatcctcc tacaatatct
     6241 ttaataattc tttcaatact taatatctaa aaataacctt ccaatgctgt ttttttaata
     6301 ttgaaaggtt atcagtacta ttatttattt tacaattttt ctattgattt taaagcttca
     6361 cttatataac cttcttgtct atctaggata ctctttgcac tatccttatc taaaccactt
     6421 tttatcataa atatagccaa tttaacgcta ttatctgctt cttctaagaa tttttctgca
     6481 acatcatatt ttacatttgt agctatcatt attattcttt ttgctctttc aaccaacttt
     6541 tcatttgatg tcttaacatc taccatcaag tttgagtata cttttcccat cttaaccata
     6601 gaagctgttg ttaacatatt acataccatt ttttgagctg taccactttt cattctagtt
     6661 gaacccatta ttacttctgc ccctactata atgtttattg gtaaatccgc ttttttactc
     6721 atttcactat tcggattcat agttattgat agtactgcac accccaattc cttagcatac
     6781 tccattgcac ctataacata aggagtcctt cctgatgcag ctataccaca tataacatcc
     6841 ttagatgtaa ctcctctatc tattatatct tgctttccaa gttctttatc atcttcagca
     6901 ccttctattg ctttaaatat tgcttgattt ccaccagcta ttatagcttg aacaagctca
     6961 aaatccactc cgtaagttgg aggacattct gtggcatcta gtattcctat cctcccactg
     7021 gtacctgctc ctatatagaa taatctacct ccctgtttca ttctatctac tattatatta
     7081 acagcttttg ctatactctc cttttgttct tcaacagcat aggctacagt tttatcttcg
     7141 ttatttatta attctattat gcctaatgta tcttctttat ctatttgcaa tgtattctta
     7201 tttctacctt ctgtaactaa attttctaaa gtttttatta acatatcatt tcctcatttt
     7261 cttaatatta aatttttaaa cacattttaa ttccaacttt cataattata tttttataat
     7321 aaattaatat tttcataata aattattttg ttgagggttt aaaattaaac ttttgccatg
     7381 gtttaatata atctaatata aataattctt cttttcttat atatcctact acattcgatt
     7441 taccactgtt tttcatatca gtcaaggcta tttgtaactc ccctgcataa tgaccatatt
     7501 cactactttc aattactata tctcctcttt ttattatttt aggagtatta aatagttcaa
     7561 atttatatcc tttatattta acacgagatt gagtagacct tatcagacta tcagatatgt
     7621 cccctctatt aaaatgtatt tcttcaaaga gtatcttctt ctcaacttct cgtatatttt
     7681 cctctattgc tacatcaaat gtaaccatat cttttctcat agaacctaat acctttagct
     7741 cctcatatgt tggataacaa tttgatatta ttatatcatt tatattattc atagctatta
     7801 tatgctttac ttgaatatct ataggtagat ttctgtgcat ctcaattgtt ggaagcccat
     7861 cagttacagg ccatggtcca aatgtagttt cattttgact tgttacaaaa actgctgttc
     7921 ttaatccata cttattaaat ctttctgtac attttataaa gtgttctaaa tttaatccac
     7981 tatatctatg tggataaaag ttatgacatc ctatcaaatt ttctttattt ggttggtagt
     8041 ccattattgt atctatataa ttagtattat tactcatatt gatttctatt ttaagatttt
     8101 gaggattaaa agtcattaat gcctcttcag aacctgtaaa tcccatatca agccttattc
     8161 cgtctgcctt cgtcttttta aaaaaattca aatctttgta gcttatttct aattcaccaa
     8221 atactctggg atttacatca agtattacct caaaaccttt aaacttagcg tattcattta
     8281 tacttttaaa ttttttaatt atattttctt tagtatcttt aactgataat aaacatgaaa
     8341 atatacgtgt aaatccattt tcagcagctt tatctatata atcatatatt tccttagatg
     8401 ttgttttttc tggataaatt gatattccta atcttcccat ctgtgtcatc tccttatatc
     8461 ctatatgtgt ttaaatttat ttcgaattac acgaaacttt tttaaaaatt tgatgaaaaa
     8521 aagtttcata tatatttata aaaaaagaaa agtttacctt ttcttttttt ataaacaatc
     8581 aacaaaatat cttcgttact tatttcataa ctgttatact ttgaaattta tactaatcta
     8641 atatagtttt taatttccaa tatagtttct aattaataag cttcttgctc ttcaactttt
     8701 tcttcatgaa gcaacctctt atcgtacatc ttgacaaatg gatagtaaat tattattgat
     8761 atagctatac ataataagtt aagaactgaa gctctccaat ctccaccagt ggctaaatat
     8821 gcccctactg gtcctggtaa tgtccaagga gcagtaacta ctactctgga tactaaatta
     8881 gcactcatag caaaccatga aactgttgcc attactattg gtgctacaac aaatggtatt
     8941 attaatattg ggtttaatac tattggcgct ccaaatatta ttggttcatt tatattgaat
     9001 attgctggta ttacagttgc ttttcctaaa gactttccat aagctgattt tgctctaaat
     9061 gcaagaagta aagctaagcc tattgtagct ccagaacctc caatccatat aaaccattga
     9121 aaaaagggtt cagcagcaat acctggaact gcttgacccg cagcatatga agctgtatta
     9181 gcatctaata attgtatcca taaaggtctt gccaatgaac ctattattga tacaccatgt
     9241 attccaaatg accaaaaaaa cgttattaaa aatattagta taattactga tggtaatgtg
     9301 tcagtggctg aaactaatgg agttactaat ttagctatga agccatgcca atcaaaccct
     9361 aggaaataag taacaatagc cattaaaatt attattaatg cggctggtgt cagagcttca
     9421 aaagaccttg tgactgatgc tggaactgct tctggcatgg ttatcttgaa tttagatttg
     9481 tcagtaaatc tataaatctc aacacctatt atagatgtta caatagcaac aaacattgaa
     9541 cctccaccaa gattagccat tggcatttgc catccttgag gaattgaatt ataccaagct
     9601 aaaagttctg gactcttttc aactatcttt aataaatcag gggtaagacc ctctattgca
     9661 gtaggaacta ttgtcaaaag gaatgtagct gtagctaata aacctcctgt caatccatct
     9721 aacttatatg actttgccaa actataacct atgccaaatg tagaatatac tgatataata
     9781 aaaaatgtca ttctatatgg tagtaatatt gtaactgcat tttcctttag atactgagtt
     9841 accccccatg aatctggtaa tggaggaaat gctaatatca agaaaaatga cccaactatt
     9901 ataagtggta atgaagctac gattccatct cttacagctc ttaagtgacg ttgttctgcc
     9961 aatctggcca tagaagttga taatttagtg tctaaaaatt ctgtaaattt atctagcatc
    10021 ttttctctcc ttaattaata tttattttta ttatcattaa aatttttata aaattaactt
    10081 tcttctaatc taacaacact gcaactacta tttactcaac agctcttgaa cttgttttaa
    10141 taactttggt cctcctaatg gactataagc ttgtgcttgt attaatgcac atggtacact
    10201 aacctcatca gcaaaagctt ttaaatagtc aaatctatgt ctaacttgtg gagctaccat
    10261 agcaatatcc catccatttt taacttcatc ctcaaactct tgagtaccaa cagccaaaac
    10321 atctatatct attccatttt ttgctccttc tttttgtaat gcattaactg ctatagcact
    10381 tgacatccct gatgaacaaa caaataatac tctcatattg attccccctg tttttttatt
    10441 tcttttcaat cattatagca tgtatttttg attacaggaa tacgtttgta tatatttttt
    10501 tcatatttta cattttaaat tgtaatttta tttcatacat ttatatcttg ctttaattgc
    10561 tttatttatc taatcattct tttgtctaaa attacacact acttaaaata ctcatatctt
    10621 ttcttaaaaa agaaaaaaga ctatcttatt attaacataa aatagtctca atactttttt
    10681 aaatatgtat tcctttatat atccagaagt aacctattat tgcaaatgat aaagcaaata
    10741 aaatatacag tctaaaaaat cccaaagaaa aacctctctt agtttcaaca aagtatcttg
    10801 tatatgcaac tgcaaaagat gtaattaatg aatttcctat aacaactcca aatctattat
    10861 ctattccaaa tatagataat aacaaaggaa aaactacagt acttactaga gatgaaacta
    10921 ttaatattaa tccactttta aaacttatag ttggaatatt ataatctgtt ctttgatttt
    10981 tctccatttg tttcttagta tttttattac tttttgtact ttttgcattt ttataattat
    11041 tatttttctt tcttttcttt tttttattat tagacatgtc atttacctcc aaaaggttta
    11101 ggtgttatat tccctaaaat aactttattt ttagcccctg ttgcagatat tacattagaa
    11161 aaagaattat ttaatgtttc attgcccaag attacaaatg ccaaagcctc ctttgcatca
    11221 gaagaatacc catattcatc ttgagttaat acttcaacct ctaataataa ctcttttata
    11281 tatcctatta aagttagatt atgagctcct ccacctccaa ttaataaagt atctacatta
    11341 acctttggaa gtatgaagtt tttataatta taatatatag cttttgccgt aaacatagtt
    11401 actgttgcta tcaaatcata tttatctaca tgtttatatt tgttaagcat gttatccaaa
    11461 taaacttgtc caaatacctc tcttccagtg cttttaggtg gcgcttgact aagatactta
    11521 tgactcatca aatcttttaa catttcttca ttaattttac cttttgatgc aaagtatcca
    11581 tttttatcat actttctatt aaataatctt tgacatactc catctattat catatttcct
    11641 ggtcctgtat caaatgcaaa tacatcatct atgttacatg atttaggtat tatagtaata
    11701 tttcctattc caccaatatt ttgaagtgct aaatttttat tgttatcact atataataaa
    11761 aattcactat atggtactaa tggagcacct tctccacctg ctgctatatc cattactcta
    11821 aaattagata ttacctttgt atttgtttca tatgctataa ctgctggttc acctatttga
    11881 agcgttgaag atacaaaatt attataactt cttggtatat gaaagattgt ttgaccatga
    11941 gatgcgataa aatccaaatt tgctatatga aagtttgctt tatgacacac tgattttaca
    12001 gctttactaa ataaatatcc aagcttaaaa tttaagctac agattaaatc aacactcgat
    12061 tcctcttcta tacagcactt tcttatttcg tcttttatat ctttaggaat ttctagagtt
    12121 ataaattcta tttgcttaac tttagtattt ctaccattcc cactaatttc agctagtact
    12181 gcatcaactc catctaagga agttcctgac attaaaccta cactatacat agttatcatc
    12241 cttttaagtt tatagttatt cctccccaaa tcaaggtatt aagtttaata attttagaat
    12301 taatcttatt tcatctttaa ttacgataat ttactcttat gaaattaatt gagttaatct
    12361 tatacacgtt ttttacctta gctaaatctt acattaattt attttcagtg tcaatgtaat
    12421 accatgtaat tttttttcac aaacaaaaca tattctctct ccaaaaacca tattaaaata
    12481 gttactacaa ataaaaaaca taccaggtac ctatctgata tgtttttcat atataataat
    12541 tattatttta taatatagat agagcatcat ttaaggcact aattaaatct tcactatttt
    12601 caagaactaa atttattctt attgtatatg tcgatttgta tatgatgata gattttatag
    12661 tctaataacc taactatcta ggcttaaata atccgatata tcataaaaat agatattaaa
    12721 aattgtatat gaaccatttt ccgaacatac ctcaatctca tgccctaaac tatctatcat
    12781 tttctttaca aaatacatcc ccattccagt agacttatac aattgatttc ttccattttt
    12841 ccccgtaaat cccttgtcaa aaaccctaga tatatcttcc tcatttatac ctataccaaa
    12901 atcttttata gataatacca ctccattttc taactttttt gaattaaatt caatcttacc
    12961 aacttccttt gaatacttta tagcattatt aataatttgg tctaaaacat atgacatgca
    13021 tttagaatct gtgtaaacat cattttctaa ttcattcaaa ctaacttcta cattattttt
    13081 tataagcaaa aatgaattat tttttataga catttttatc aatttttcta aattaaattt
    13141 atttataaat acatcttcaa acatagtgtt gcttctacta ctatacaaaa tagagtttac
    13201 taaaaaattt atctttgcaa cctgattctt aatatccaaa ctatcttcta tgctacttaa
    13261 cctatctgta attatactta aagaagatat tggaattttt atttcatgaa cccatttagc
    13321 tatgtactca tccaattctt ttaatgattg tttataagtc tctatgtcac tattattttt
    13381 tatttttaag ttttctataa tatcttttat tagattttct tcaaaagatt gtccatcaac
    13441 taaataactg tctatttcac cactattttc tatacaatta tataaatttg tgtaactatt
    13501 tctaaaattt atataatcta taacaaaaaa taccattatt ataaacagta ctaaaaaatc
    13561 taaatatatt aactctgaat atttattttt aaaaatatct atagcaaata agtagatatt
    13621 tattatacta actacaacta aaggtagagc aatatactta gacctacctt tcaaatattt
    13681 tactagcatt gtattatata tcctaccccc ttcttagttt ttataaaatc atcaaaacct
    13741 agttctttaa ctttacttct caatctattc atatttacag taagtgcatt gtctgtcaca
    13801 aactcatcat tatcccatag agtcatcata agactttccc ttgatacaac cctattctta
    13861 ttttttaata ataatgttaa aatttttatt tcgtttttag tcaactctat acttttatcc
    13921 ttatacttat gttttataat ccctttcccc acatcaaaca tcaaatcttc acaatacaaa
    13981 atactattag aatcactata attatagact cttcttatta taccatttat tttggtaacc
    14041 aatatttcta tagaaaatgg tttggtaatg taatcatctg ctccattatt tatacccatt
    14101 attaaatcca tatcagaatc tcttgatgat aaaaatatta atggtacatt tgaaatagct
    14161 cttatttttt cgcaccagta aaatccatta tataatggta aatttacatc taaaagtact
    14221 acatctggtt ttttatctat aaattcatct aatactacct caaaatcatc aattaattct
    14281 actttaaatc cccatttttc aagtgctaat gcaatctctt ttgataaatc aatatcgtcc
    14341 tctactaata aaattctata catttgtata ttctccatta tcctaatatt tcactatgct
    14401 atgaattatt aatatcataa catattttat atgaatttat tacccaaatt atttagttaa
    14461 tcaacaagga gactcttatt acaagaatct cctttctctt tattaatata tcaactaatt
    14521 tttatacact acactcttaa acttattaac agatactgct ccatatatta tgaatattat
    14581 caaacatatc actaaactta gtatatatat accaaataaa tcggtcatca ataaatctcc
    14641 tgctaatctc aatgcaaaat aactagatat agttgcactt aacattggca aagtataaaa
    14701 catagctact tctttaagta tagctctatt tatatctttt tctgtagtac ctatttttaa
    14761 caaaatctta tatttctctt tatcttcata tgcatcacta tatattttca tatatattat
    14821 acttccagct acaattgcaa agactaagaa taaaaacaca agcactacat atgcaaactt
    14881 catccattga ctctcacctt gagctttata tccattttga atatacccat tttttaaact
    14941 taaattcttt tcaatattgt tgaacattac ttgactattt tcttcttgaa atacctttgc
    15001 accataaaaa tacacttgtt gacccttatc tcttagttta aaatatgtat catcatttac
    15061 cactataata ctttgattaa gtcctgtccc tagtactgga acttttactt ctccccttga
    15121 aatattatat aactgaccat caagttttat cttggcttct tttgtagcta ttgatgctat
    15181 cgtttgaggg ctttttaact ctattacatt atttccacta actaatcttt caccatactc
    15241 ttttaattct ttatcattgc cattcacttt aagagtatta caaaaatcac tatatttcat
    15301 tatagtatct gttgtatcat aagcttcttt tatatctgtt ttctttaaaa ttgttagttc
    15361 tggatttact gagtacttta tcttattagt atcttttatt atattctcta tttgtttttt
    15421 attcacttca tctttatcca ttgaaactat gctaatagtg tacacatttc tttgttcttg
    15481 ggcattctta tgtatacgct tcatcgcaac tgaagcacca agagttgata ttgttgtagt
    15541 tataattata gctattatag cataagtttt atagttttta tttaatcggt agtttatatt
    15601 gtttattgca attatattat ttccattaaa taatattttt ttatttttga ttataatatc
    15661 aaatataaca ggcataactg atttgaataa tccataagta cctactatta ttatacctat
    15721 aacaggtagt acactagtta tatctccagt tcttatattg gtagaaagat agtagccata
    15781 tcctattact ataattgata ctatagctaa tatatatata cctattccta cctttggtat
    15841 tttttcttgt tttttagata tattgattag atttattaca ctacttctac atattgttat
    15901 aaaacctttt acagccatta ttataaatat agaaataaag attaaagtag catatagtat
    15961 agcttctata ctaagtccat tagatacctt tatattaaat ccagataact ttattatcac
    16021 tacttgaaaa aatctagatg ttattatccc tataattatt cctgttatac aagaacttat
    16081 tcctataagc atattttcta caaaatatat ttttcctata gtatataaat ctaatcccat
    16141 aaaagacaat attccaattt ctttagacct attcttaaaa aatatatttg ttgcatacca
    16201 tataaagaaa aataaaaata ctgatagtat cattattaca gctactaaca ctgatagtgt
    16261 aaatttttta ttttcatctt gtaacacatt tatagcatca ccatatctta ataattcaaa
    16321 attagttagc acaactactg aaaaaaccat agatataaaa aatgctagat aagttcttat
    16381 gttactctta aaattattat aagcaattcc aagtatactc acttacatct cacctcccat
    16441 agaggctagc atatccataa tttttccata aaattctttt ctactatttc ctttgtttaa
    16501 tttacacttt atatttccat catttatcat atatacttca tttgaataac ttgctgttag
    16561 agggtcatgt gtaatcatta taatagtagc gttattttct ctgtttattt tttctaatga
    16621 ttctaaaagt tcatatgctg atttagagtc taaagcacca gttggctcat cagcaaatat
    16681 gactgttgga tttgtaatta atgacctagc tgctgcgcct ctttgttttt gtcctcctga
    16741 taactgatat ggatatttat ctaaatgatt ttctaatcca aattttttag ctatagaaaa
    16801 taccttagct tctatctcct tacttttctt ttttcctaat gctagaggaa gtgctatatt
    16861 gtccattagt ttcatattgt ctaatagatt atagtcttga aatataaacc ctattttttc
    16921 tcttcttaat tttgatagtt cattattttt tacttttgat atattaattc catctagtat
    16981 tatttctcct gaagtttgtt tgtcaagtgt agacattaaa tttagcaatg ttgtctttcc
    17041 tgcaccagat ggacccatta ttgatataaa atcaccttca ttgactgtaa gagatatatt
    17101 ttttaacaca tttgttttaa atccttttat tccataatct tttgatatat tttttatttt
    17161 taatatttct ttcataataa tagcctccag ttgctaattt tttaatatat atttatttta
    17221 aaggttttat ataaataaat ccttaagtaa atattacatt ttaactgtta gcattacact
    17281 tttgtaacat atggaagact attataatta tagtagaaat atcttaaaat aaaagcatga
    17341 tttacttctt tgtataaaca aagaaaagaa gctcctaaga agcctctaat ctatacaatt
    17401 attcttctct ataaaaaacc aaaatacaca ttgattgtgg caaatacaga tactataaac
    17461 aagtgatatt actactatat attaaaacaa ttattcctga agataattca ataagactat
    17521 caagcaataa gctaagcatc caaaaagact taataccatt aatgtataat tatacccaat
    17581 attaatgctc aaaaaaccag caacagttgg aataatcaga gatgaacctc ccataaatat
    17641 acttaaagct attaattggc tactacttct aagtttttta ggagaaactt caaatattaa
    17701 tctttttgaa gtaattaaaa ttaaaggagt agaaaacact tgaaaaacac acagtataat
    17761 aatgatattt gaatcatttg ttaaagcaaa taatagaaac tgaactgtta tcataatagc
    17821 acttaatttt aataagttta aactgctata tttttttaat aaataagaac cagccagata
    17881 tgttggtatc tctaacaatg actgtaaaga ccactttaaa gatatctgag aatttgtagc
    17941 atttaaagct atcattttat ctattacagt acagttattt gctaccacca ttgagtataa
    18001 taagaataaa atacatacta gtaagatata ttttttatct tttaataatt caataatatc
    18061 tttattagtt acatttattt tcttattaat cttttcaata tcaggtaata atagtatatt
    18121 tataataagt aatactacaa ttaaaccaat cgctacagat aatccaaaat atgagaatct
    18181 aagaatcaag taagatgcta ccatacttcc aatagcccat ccaatcgacc caaaagtctt
    18241 aataaatgac aaataattat taatattatc tccacaacct attatccatg tatcatataa
    18301 actacagcaa gtatttaata atcctcctga taaagcaaca agtaataagt atattataaa
    18361 agttgcagat tcagtagaaa acataataac tgatgcaatt ccataaactg ataaactaat
    18421 taaaacaatt tttttcattg tttgatactt atcagctaat actccaaata ataattgaaa
    18481 caatatattg gttattgcat agctcgataa tataattcct ctttcaaacg ctgagtaacc
    18541 taattttgat aagaaaggtg tgaattttgt tgaagcaaag gcaaaaataa tatatgagaa
    18601 aaaatagaat atgataaatt taaatttaat cgctttatta atcataatca atcaaaattg
    18661 ataactttaa taccactatt tccttcctga tttaagtttg aaagatgata ttttaccgta
    18721 ttaggattct ctacaataac taaaggaata tataaagtat tttcaagtaa cttattatca
    18781 atttctacta acttttcccc catattaact tcttgacctt gtttaacttc taatttaaag
    18841 ttttcttttt ttgttgttgc actatccaat ccaatatgaa taagaatttg aacatcattt
    18901 tgcaatttaa ttcctatagc atggttagtt tgagcaataa aacttattac tcctttagtc
    18961 ggtgatttaa gaatattgtc ctttgctatc atagcaaatc catctcccat taccttagtt
    19021 gaaaatactt tatcaggaat ttctccaaga ttaattattt ttccatcaat gtatgaatta
    19081 attgtaattg cttctttttt tcttttaaag aacattatag acactcctct acataattaa
    19141 ctttattcta attatcatta caaataacga tattaagcat actgcaattg aggaaaaaag
    19201 agccttatag ctctttcccc attgtagttc tttaatactg cttatgattt gtttaattcc
    19261 aacaaattta taaatatagg taaacatgat aacaatagtt attgtgtaaa tttggaatat
    19321 ctgccctaat aagactaaat agaaagcaat catgttttct tttatctctt tattcttttt
    19381 tatattgtaa ataacatatg cactgtatac tgcactaaag ttagagatta aatttaaact
    19441 tgcattaaca tcattgttta aaagttgtga aaatgaaata tttgtaaata atgatgtcaa
    19501 ttctaaatac actatatata aaacaggtaa tattagaaaa caaaccataa ccactgaaag
    19561 aaatatattt tcatctttta atactgctgt gatatttttt attttatttg ttatcatgtt
    19621 tttgttctcc ttatttaaaa gttggccaga aatccttatt agccacaatc atttcatcta
    19681 agatttcttt tgctactttt gcacttggaa cagttttact catagtaaac gactgccata
    19741 atttttgata acttttttct atccatgctt ctacagctaa tttttcagaa gcaacttgtt
    19801 gttccattaa acctttttgg aaagttggaa ttttacctat agacataggt tcaaccatat
    19861 cttttccaac aaggcaagga atttcaacca ttgcatcatc tgcaaaattt tcaatagctc
    19921 cattattagg aacaatcagt aaaaatcttt cctgagtatt aaatgctaat gcacaagcta
    19981 attcaacaat aaattgagaa tgttcacttg catcaaacca tgtatctctt gctgtgccat
    20041 tttttataat tctttcacat tcttcgaatg tatccttttc tcttctatcc ataatttcat
    20101 ttgctcttgt ataatttgga ttagcatgtt caacagtttc atcagccatt aaataatatt
    20161 ttaaataagt attaggtaat gtagtcggtt ctatttccaa taaatccttt gctgctctca
    20221 tagtttctaa ccaactagat tctttgtgtt gaccttgagg tgtaaattcc tcatatcctt
    20281 ttttagcaca atattcctgt aatttaggca ttaaatcatt tccttctttg tccttaatac
    20341 ttgtccacca cccgaaatga ttcaatccat aatatctaac atcaaagtct ttacgagaat
    20401 ttaaacctaa aactctagca aatatttcct ctaaacatac tggcatatca caaatattaa
    20461 taattcttga atttggtctt aatacacgac atgcttctgc aactatagct gctggatttg
    20521 aataattaag catccaagca tttggagaat atttttccat ataatcaatc atttcgatta
    20581 ttggacctat tgttcttaat ccataggcaa ttccacctgc cccacaagtt tcttgaccaa
    20641 caaccccata ttttagtgga attttttcat cttgttcacg taaagcatat aaaccaacac
    20701 gaatttgtgc cattacaaaa tcaatatcag taaatgcttc ttctggattt gttgtataac
    20761 taaatttaat ttctggattt ttctctctaa caattattgc acatgcttca gcaacttttc
    20821 tttgtctctc atcattatta tcatataatt taattgattc aataggaaat ttagcaacac
    20881 tttcaattaa cattgttaca attccagcag tataagtact acccccacca gcaattagta
    20941 tcctctgtct ttttctttcc atctttcctt ctcctttata ataaattttc aaattcttct
    21001 cttacacttg gtactgaaag cccaataata atctgaatag cttttccatt atgcactaaa
    21061 ccatgtgcac ctactgcctt aaaatcttct tctttagaaa taattgagtc atctttcaag
    21121 gttaatctca acctcgtagc acagtttgta acatctacaa cattttctgc tccaccaact
    21181 aattttatgt attcagatgc ttgttgagag gctttactca atttatttcc tttttcatct
    21241 ttagtttcta agtattcatt tttagtatat agttttgttt cttcactatc ttcctctcta
    21301 ccaggagttt tcatatcaaa cttcttaatt aagaaagtaa atacaaagaa ccagataaat
    21361 gaaaatataa tccctacaac aacttgaaca acatatacct tccagtgata cattccaaga
    21421 ggaagccaat ttaatacaac ccagttaata attcctccac caaaatcacc aacaactcct
    21481 agggcaaacg aaatcgtttg caagcatgca tctaaaaatg catgtactag gaataaaagt
    21541 ggtgctacaa ataaaaatgt aaattctaac ggctcagtaa ttccagttaa tactgcagta
    21601 agagtagcag gaatcattaa acctaatact tttttctttt tttctggctt tgcagttaca
    21661 tagaaagcac caaatacacc taatgaacca aacaccttcg ataatccgga taatgaaaat
    21721 ccgattggat acatttcctt tagagtctta gctgaagttt gaaaatcctg aatatgagta
    21781 gcccaatata ctgatgtacc acctggaaca acaacactat cataacaaat tggtgcatat
    21841 acaaaatgat gtaatccagt tggtattaaa atcttttgta agaatgcata acaccaaact
    21901 cctatatttc cactatttat aaaaaacgtt tgcaaatgtc taataacatt ttgaatatga
    21961 ggccaaataa aacaagttaa taatgcaact ggtatcataa caaagaaaca aattgcaact
    22021 acaagtgatg agcctctaaa taatccaata aaatctggca gttctttatc ataaaatcta
    22081 ttatgaatat aaatgacaat acctgatatt aacaaagcac caaccatacc agtatctaat
    22141 gttttaatac cagcaatgct tacaagtcca cttccagcac ttatttcagc tgcgaaatca
    22201 actccaaagc tttttcccca aaaacttaac atagaaccta caaaataatt aaaagtcaaa
    22261 tacgttgcta aagcctccat acaagctctt gcttgttgct tttttgcaag tgctattggt
    22321 aaagaaatag caaataataa tggcaattga ttaaatacca tccaagctcc actagatatt
    22381 acaccccaaa attggtacca aatattttct ggtgtagcaa gtgaacccat aattgactga
    22441 ttttgaaata caattgagaa tccaaccatt atcccagcaa aagcaaataa tagtgttggt
    22501 gtaaacattg cccctccaaa acgctgtatt ttcttcatca tataaattac tcccctcctc
    22561 atatttttag tttatctttg gccttagata tctattattt taacaggtta ataaatatat
    22621 acaatcttta gggtacattc ggagcttatt cacagtcaaa taagtttttg cacattttta
    22681 tgttcaaatt ctcataaaaa aaaagaatga ctacaatttg caatcaatcc cttctgtaat
    22741 cattctatgc cttttataaa tactatattt aataaataat aactcaatca atataaaata
    22801 taacattgtc aatttataac tagggtgcat ttgtaaaaat gagatattag tcgctgaaat
    22861 ataaattgat tcatcactta aatcagtcaa tgtattattt ttaaactctg taattgaaat
    22921 tattttagtt cctattaatt ttaatcttct agcaatatta agtgtattct cattctcacc
    22981 acttagggat ataataatca taacatcatt tttactgaca atattgtaaa aagaatcatc
    23041 aaatgttctt ccctcaaaat catatattat ttcttgacat gataagaaca ttctctttat
    23101 ctgttttgca accacatttt gaatatcacc tgaaccataa gcgtatattg ttcttgattc
    23161 ataaagaagt ttacacaatg catcataatc tttatttttt tgatcttcaa catacttaat
    23221 tattgaatta catgcaatat caaatgactg attttctatg acatgaacct tttctgattc
    23281 ccagcttaat aatactttaa actcactgaa accttttaaa tctaattttt taacaaatct
    23341 tgtaatagtt gcacgagaca cattacattt catagcaagt tcatttattg acatcttact
    23401 acaatgtttt ttatttgcag ttatatattt ccatattaat aaatctgtat cattcaattt
    23461 attataattt aaaaaaatca tttcttctaa tctcataaac tattaccccc tttaattaaa
    23521 aatcgtcata aatattatac caatattcaa cattaaatta cagcgtattc ttatatttac
    23581 tatttagccc ataaaattga ctataaaaaa tactgctata attgatgttt aaaaaaatgt
    23641 agttttaatt ttattctttt atctaataat taatattttc aagttctagt gcttttctta
    23701 tacaattatc atctttaaaa tcagcagtcc ttttacaatc ttttacttta aattctggaa
    23761 tagttttaaa ttcctcatca catattccat tcttatttaa atacatacaa cttgccattc
    23821 taactattag accactattt tttaaattaa acaatacagg gtctatacac cctccatctc
    23881 cgccagtagt ttcaccaact aaagtcgcaa atttattttc tttacaaaac attgcaaatg
    23941 actctgatga tgaatatatt tttctatcaa caagtaagta tatattaccc ttaaattttg
    24001 aatcatctgt agattttata gtataggaag tatcttcaaa atcattaaaa tcttttataa
    24061 tttctttagg tgcattttcc tttacacttg ctggtaaatt ttctacagga tttaatttta
    24121 tatttcttgc atttgtatat ttttttacta tttcattatt atttctataa attctataac
    24181 catttatttt gacatcattt tttatgagtt tagatacgat tccctgccaa tacctatcac
    24241 ttccacctaa attacctctt atatcaataa ttaaagcttt atgtttttct aaagtgttaa
    24301 tataatctcc aattattttt aaatcattat ttatagagcc attctttgat gacattgatg
    24361 gtaaatacat atatccaatc tgacccttaa taacatcctt taaaattaat tcttttttta
    24421 caaaaatatc atctggcatt tttattttaa gattcataga gtcatatcta tccacaactt
    24481 ttttatcatt aagaaaatca taccaatcat tattagagta tgatttctta aaaagttcat
    24541 atcttttctt attatctatt acttctgtgt gtttattatt taaatcagat aatatagatg
    24601 acagttcttc tataaattct tcatcatttt cagtgttttt tattcgtttt gtatattcat
    24661 ccttatttgc taaccaatca atattattaa gcctcttatt tacacctaga tatggatatc
    24721 ctgtctttac aacattatac acataattaa agtcatccac cttttcctct tcagacaact
    24781 gattcttttg agaataagta gaattaccta ataatattat aaatgctaaa ataattacta
    24841 aaaataatac tttctttttc atgtaattct catccacttt cagattttat attttaatac
    24901 attgtatcat tattttatta tcacctaaac agttaaaaga aattcttgaa gatatattcc
    24961 actattattc tctttaaaaa tttttatttg tattttcaaa attatttcat gaaagttata
    25021 aaataacaat tatattcaaa tatctatttt actgctatat cctgaaagta aattcttatc
    25081 agtgatatac taaaaaagat atgctaaaaa atttatccat tataatgact taaatttaga
    25141 tactttactg atttttattt attagttgag aaagatattg ataattaaaa atattgataa
    25201 ctatagaaat gattattttt tatagaaatt ttaaggatat aaactaatta tacttaagtc
    25261 tatatcctta atttatcatg attatgagaa tttttacaaa tacttatctt tgtccgtttt
    25321 agaataaaaa caagaacata tcctgttatt ttattcaaac atccaagttt aattactaca
    25381 caattactta ttagaaattg tatctacttc atatatatat ctgttaagta tagcttctag
    25441 catttcttgt cttccagact tattctttat aactggcatt tgcagtgcat aagattctaa
    25501 ctctttaaat ccaaccttac cttctactat atccttacct ataccttcac taaaacttgc
    25561 atatctttct ttagtaaagt tttcaaatac ctcatcttct aataatttat gagctattaa
    25621 taatcccttt gcaaatgtat ccattcctgc aatataagct aggaataagt cttctacctc
    25681 aaaagatgct cttcttactt tagagtcaaa attaaaccct cctggtgcta ttccaccttg
    25741 ctttaacact tcatacatag caagtgttgc atcatatata tttgttggaa attggtctgt
    25801 atcccaacct aataataggt ctccttgatt tgcatctata ctacctaaaa cattgtttat
    25861 tcttgctata tttaattcat gttggaaagt atgtcctgca agtgttgcat ggtttgcttc
    25921 tatattcatt ttgaagtatt tatccagatt atactttctt aaaaatccta aaacagtagc
    25981 agtatcaaaa tcgtattgat gtttagtagg ctcttttggc ttaggctcta ttaagaattg
    26041 tccagtgaat cctatttcct tagcatagtc tacagccatt tgtaagaatc ttgcaaagtt
    26101 atccatttca agttctgtat tagtgttaag taaagtttca taaccttctc ttccacccca
    26161 gaatacaaaa ttttctcctc ctaagtactt agttatttcc atagcttttt taacctgagc
    26221 tgctgcatat gcatatacat ctgcattaca tgttgtagat gcaccatgaa caaatcttgg
    26281 atttccaaac atattggcag ttccccataa taactttata cctgtctttt ccatattaac
    26341 ttttatatac tcaactattt catctaatat cttatttgtc tcttctaaat ctcttccttc
    26401 tggtgctata tctctgtcat gaaaacagaa atactcaatt cccatcttag acataaattc
    26461 aaatcctgca tcaactttaa tctttgctat ctcaattggg tctgttatat tattccaagg
    26521 tctctctact gtgcctactc caaatgggtc attaccctct ccacataaag tatgccaata
    26581 gctcattgca aatcttaagt gttctttcat agtcttatta cctaccactt gttcaggatt
    26641 gtagtatcta aatgctaatt cattgtcaga ttttactcct tcaaatttta tctgtcctat
    26701 tcctttaaat atttcactca tttcatgtcc tcctaatatt ttacacatat aatatatgta
    26761 gtatgttttc ttaatttaaa actattttct atctttattt ttctttttaa gctaatttaa
    26821 ttatttattt ttgcattaac tacttcatct aataacttaa agtcatcttt caaagatttg
    26881 tatattcttt tatacaatct atgatatata ttgtatgtat ctacattttc acttattggt
    26941 tctaatttag ttgaagtttt tattatagca tcacaagctt tttgtatatt ttcatatatt
    27001 ccagctccta ctaatgcaag aattgcaact ccaagtgctg gtccttcact tgaatttata
    27061 gtatatacat cttgattaaa catatctgct tgcatctgtc tccatacttt actttttcca
    27121 cctccacctg aggctcttac ttcattaacg tctatattaa gattctctat tatatccata
    27181 cagtctttaa gactatatga aacaccttcc attattgacc ttgcaatttc ttttttagaa
    27241 tggattggag ataatccaaa gaacacacct cttgcataag ggtcaagatg aggagttctt
    27301 tctcccatca tatatggtaa gtaaagcaat ccattacatc ctgttggtac tttacttact
    27361 tgattattta taatctcata tacatcttca cctaaagaat ttgaaacttc cacttcactt
    27421 tgacagaaat tatctttaaa ccattttaat gacaagccag ctccttgagt aactcccata
    27481 acatgccatg tatttggtat tgcatgacag aaagtatgaa ctctaccttc tttatcaatt
    27541 tttggctctt ttgtataagc aaatactaca cctgatgtac ctatagtaga tgaaactact
    27601 ccatctttaa ctattccatt tcctatagct cctgctgctt ggtctccagc tcctcctacc
    27661 actattgttc cttctttaag ccctgtcaaa cttgatacat ctctagtcac ataacctgta
    27721 atctcttgtg attcataaac tttacctaaa agatttttat ctatatttaa cttttctagt
    27781 acttcatcgc tccaatctct ttttcttata tcgagcaact gcataccact tgcatctgat
    27841 acctctgtag catattcacc tgtcaatttg tatcttatat aatcttttgg aagaagtatt
    27901 ttattagtat ttttatatat atctggttca ttgtttctaa cccataatat ctttgatgct
    27961 gtaaatcctg taagtgctgg attagcagtt atttcaatca gtctttcttc acctactaaa
    28021 tcagttatct cctcacactc ttttactgtt cgttggtcac accatattat agagtttctc
    28081 aatgttttac cttcattgtc cagcataact agaccatgca tctgccctga taatccaatt
    28141 ccttttatac attcactgct tatatttgaa ctttgaacta cttctcttat agattcgact
    28201 acagctttcc accaatcatt tgcatcctgc tctgcccaac ctgatttctc ttgtataaga
    28261 ggatattcat atgtacaact ttttatagta tttcctagtt tatcaaataa aactgttttc
    28321 gtacctgatg taccaatatc cacacctaat acatactcca acttcatccc tccttgaatg
    28381 ccacttcata actatcgttt tcctatccta atttttttat attttaggta tattttactt
    28441 gctttatttt tgttagtata ttaaactaac ttatgaacta ataataaaat atttaattat
    28501 ctttgtcaat gcaaaataac cttttatttt tgtgtaaaaa gtattacaat attattttaa
    28561 ggaggaatat caaattggtt actgataaat acactattcg agaaatgaat gaaagattgg
    28621 tacttgaaca aattataaaa aatggtccta tttctagggc aagtatagct tctactatag
    28681 gattgaataa agcaactata tccgctatta ccaaaaaact tattgatgaa tctctagttc
    28741 atgaaatagg aattggaaat agtactcata gtggcggaag aaaacccata ttattagtat
    28801 ttaataaatg tgcagggatt tctttaagta tggacattgg atacgactat atattttctt
    28861 ctctatctta cttagatgga actattataa attctaagaa attaacagat atacaagtaa
    28921 gtaaagacaa tgttatacaa ttgatagatg aaattataaa tagttataac atatccaaaa
    28981 tagacactcc ttataaggta attggtctta ctttggctat acatgggatt acttgtgaaa
    29041 ataaagtatt gtttactcca tattacaatc ttaatgagat tgacttatat agcatcctta
    29101 gcaaaaagta tgactttcct attcatatag aaaatgaagc aaacttaaca gctttagctg
    29161 agaatacttt ttcaactgta cataactcac tacttagtct cagtattcac agtggatttg
    29221 gttcaggcat tataataaat aataaattat atagtggcag aaatggtatg agtggagaaa
    29281 ttggtcacac tatcatcatg cctaacggta aactatgtcc ttgtggaaat cgtggctgtt
    29341 tagaacagta ttgttcagaa aaaaaggtat ttgaacagct ttcttcttta gaaaatattc
    29401 ctaaaataga ttctgatata gtaaagcaac tatattatga agataaccat aatgccaaaa
    29461 aagttattca tgagttttgt agttacttaa caatagctat aaataatgct attacaactt
    29521 atgctcctga aataatatat ttaaatagcc aaataattag tgatatccct gagatattac
    29581 agattacaaa agatatgtta gttagctctt ttaacaaagg aataaatata gaaatttcat
    29641 cattagggtc tgaggcatct ctatatggtg gctctgctgt aaatatcaag agctttttga
    29701 atatacaaaa tttaacttta attaatgaaa tataagggtt atgactatat acagccataa
    29761 ccctttaaat tatacattct aaaattagat ttattatata gtttatgcaa gattagctat
    29821 attttttaaa aattgtatat caattaagta ttaacataag tatttcgttt taaatattaa
    29881 tataatcttc ctactcatca tcagataata aatttgcatt ttgttcaacc atagaaatat
    29941 tatctttacc tattttctct aattttgctg ccaattcctt tgtatctaaa aaagctgatt
    30001 caataaccat acctaaattt agccctgaaa taattttaat tcgactgtca ttattggcta
    30061 ctaataaagc agcattgcat ggagttccac ctagtaaatc tgccattata attatttctg
    30121 aactttcctt gtacacttct aaagcttttt ttaatttttc tattgttcct tctattccat
    30181 catcttgttt catatttaca gtaggataat ctatagaatc ccctattatc atttttgcag
    30241 aatttaagac ttcttcagcc atattgccat gactcatcaa aataatacta ttcaatttaa
    30301 tacacctctt taaatctata agatgtctac aagataagtt ttcttatcat caggcgtcat
    30361 tatagttgta atttcaattc cttcatctaa aagtctttta aatgcttctt tctcttcttc
    30421 agtaacactg atattaggtc ttagaactgt tgtatctttt ctacttgaca tatttcctac
    30481 atttatatta tgtattttaa atcctgcctc tacaaatgga ataaggtcca caggagactt
    30541 aacaattaat aatacatttt gtccttcata ctttcctgca gtaatatttt ttatagcagt
    30601 ctcttctgta attatagatg acgctatacc tgcaggtgta gccattctta aaacttgttt
    30661 ttgaacagaa ttgttagcta ctgcatcatt tactaccatt aatcttgtaa cacccaattt
    30721 attactccac atagttgcaa cttgaccatg gattaaacgg tcatctatac gaatattttt
    30781 aatacctttc atttccatat ctaatatcat cctttcctta ttttaaaata cctaatgcat
    30841 ataaaattat tgaaacaacc attactccta aaattaagta tataggtttt atattctttt
    30901 taagagccca ataaacaatc acaactaaaa ttgcaggaac taacccaggc ataatttgat
    30961 ctaatatttc ttgacctttc attgtaaaat caccttgttg aaagttcaaa gcaacattag
    31021 cttttacaac tgaaggtatt aatgcaccta caaccattaa acctaaaata gatgtactct
    31081 cagttaaaat ctttaaactt ttttcaattg aagatattag cttagtacct gatgtataac
    31141 cagcattaaa tagtggtata cttaataatt tcaatgctac actcactaca atccacagta
    31201 agattccaaa tggacttcca tctatcgcca tattagctgc tatagctcca aaaattgtcc
    31261 acggaataac gacaaataaa ctatctccta aacctgctac tggtcccatc aagcctgttt
    31321 taattgaagc aacagcctct tcagtctctt cacttgataa ctcctccatt gctacattaa
    31381 tacccaaaat ataaggggca acccaaggat tagtgttaaa aaattgtaaa tgatttaata
    31441 cagcactttg taacttttct ggattatctt tgtatactac ttttaaaaaa ggtagtactg
    31501 cataacagta tgccaaggcc tgcatcttct cataatttga agaaacagaa ctagtaaaaa
    31561 accagcgcca taagacactt tttttcacct tttcatttga tttatttaat gataccttat
    31621 cactcatctt cgtccatacc tcctactaca gtatattgtt gttcttgatt ttccaaattc
    31681 tttttgtaaa ttataagtgc aattgctcca ccaattaaag ctacacctaa taatggtact
    31741 ttcatatata ctgctaaaac aaaaccaatt actaagtaac taaaatactc cttagcagga
    31801 agatatctaa gtaataatcc tatccctact gctggaagta gtcctccagc tactgttaaa
    31861 ccacctgtta accattcagg tgtatatttc aaaatggtat taattaaaga aggtccaaat
    31921 attacagata gagcaactgg aatacctgtt gtagctgctg ttaatagtac tcctaacatt
    31981 tgcatatagg ttatattttt ataatttcct tttttagctc catcttctgc tttatgaact
    32041 agccatatac caatagtatt tctaagaaca tctaattgta ccatcaataa agcaactgga
    32101 atacctatag agattcctac agatgcttct tgcttagtcg taatagttat aaatgtggct
    32161 actatacttg ctgtttgata atcaggtatt gatgctccac caaagttact aatacctaat
    32221 gataatagct gtaatgtacc tccaatatat aaccctgtat ttatatctcc taagataaga
    32281 ccagtaataa aacctgctgt tactggttga tatgttccaa acattgttga atttttttca
    32341 tagtttataa aaaatccata tagtacgatt aataataatt gccataattg tggctccatt
    32401 atgatttcac ctctcccaaa atatttttta gttctttaaa attataactg gaatcttgct
    32461 ttaggaaaat aggaatttct tccagtgggc attcaacttc ataagttttc ccaccttcat
    32521 aactaactcc actaaatata ttttcccatg tatgtccttt tggtaaatat acttttctac
    32581 ttctatcttt ataattcatt attggagcta ctaaaatttc atctccaagc atatatgtat
    32641 tatccacctc ccaagtttta tcatcttcag gaaattcata aaataaagtt ctcataactg
    32701 gtgttccatc ttcatgcgct tctttcatca gtttttttaa ataagttttt aagctctctc
    32761 taatttttat gaattttgta agaattactt caacttcttc accataagac catatttcat
    32821 taggagctcc agttaccatt gagcctccac ctttatttga taatggttta ctatgaggca
    32881 atctatctcc atgcattctc aaaattggtg aaaaagtagc atattggaac catcttacca
    32941 taagttctct aaattctggg tctttaggat ttcctccatg gaaacctcca atatcagtag
    33001 tccaccatgg tatacctgcc agtcccatat ttaaccctgt atttacttga ttattaaaag
    33061 cttcaaaact agaatcaatg tcaccagacc atactaaagc tccatacttt tgagctcctg
    33121 cccaagctcc tcttacaaga gttacaacag actctatgtc attatgtaaa ccatcataaa
    33181 tcatttttaa ataatcaata ggataaatat taccacaact taaaacattt ccttttttat
    33241 atctatagtt atcaaaatca tacacagaat agccaggttc agctacatct aaccaataat
    33301 agtcaatccc aagttgttta taatttttat ctattctatc ccaaacatat tctcttgcat
    33361 tttcatttgt catatctaca aagactgtat ttccttgaat ttgcattgtc attctaacac
    33421 cacgattaac attgactaaa tagccatttt ctaaataatc attgtagttt tctgcatcac
    33481 tttgaactgt aggccataca gatactattg gttctactga atactcttct ttcaattttt
    33541 taactaattc tttaggattt ttccaataat ctaagtcaaa tttatattca ccttgcttag
    33601 gccaatgaaa atagtcaatt gcaattgatg aaagcttaat tcctctttta gaatattcct
    33661 tcacaacatc taaaacttct tctgaagttc tatatcttag tttactttgc catagtccta
    33721 aaaggttttc aggcatcata ggaacagttc cagtaacttg agagtaattt ttatttaact
    33781 cttttgggct ttctccacaa gtaatccaat aatctataaa atttgtagaa aacatcttcc
    33841 attctgtcat atttttagca aaagatactt ttccaattcc cggattattc cataaaagtc
    33901 cataacctag actagaaata taaaaaggaa ctgatatttg agaatttctt tgggctaact
    33961 ccaaaacagt attctttaaa tctaaaaatt tatgttgata ttgtcccatt ccaaaaatct
    34021 tttcagaagg ctcagattca aatctagtag ttacttcaaa ttctccacta tagcttggtc
    34081 gatattctct tgattttaac tttagagtag agttaaagtc tttggtaatc tcaattgtcc
    34141 ctacatcttc tccaccatca tcatgtttta cagctcttaa tcgaatatat tctttcaata
    34201 atatttcatt tttatcatta taaaaagtaa ttctatctct atggtctaag acagctttta
    34261 tctttccatt tttaatgatt gcacttccat cttcattttt attaattgta atatcatctt
    34321 tatttaactt aggtttttcg ttaagagcat agttttcatc aacaaaattt tggtctacaa
    34381 acgacctaat acgtaaagag ttttttcccc atgcttctat tcgcaatagt tcattgtcaa
    34441 atcttttttc aatatagtct tcagatattt ttatcacggc tacctcctaa atcaatggta
    34501 taatattcta tctttgtgta acttaagtta aataatgtcg tttagtatcg ttatttataa
    34561 attaggtttt atatataatt catttgtaca ttaaactaac ttaccaataa tatactttat
    34621 ttgactttgt ttgtcaatcg ctttccggat tttagtaaaa ttttctaata tattgctgga
    34681 aagttgttct taatatcttt tatttatact tttaatatct gtgatgatac ctatatacat
    34741 ctagtttttt taaaacacat acaggaataa catttgccta ttatgtagat aatatcggac
    34801 ttgaaaatat gtatagtgac cttataaata cagataataa tatattaga
//
LOCUS       DAYATN010000027        34743 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.027, whole genome shotgun sequence.
ACCESSION   DAYATN010000027 DAYATN010000000
VERSION     DAYATN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.027"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(441..2828)
                     /locus_tag="ACGI8A_001286"
     CDS             complement(441..2828)
                     /locus_tag="ACGI8A_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFH0179289.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKAYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="ACGI8A_001287"
     CDS             complement(2851..3216)
                     /locus_tag="ACGI8A_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFH0179290.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="ACGI8A_001288"
     CDS             3415..4263
                     /locus_tag="ACGI8A_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179291.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGIPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="ACGI8A_001289"
     CDS             complement(4700..5788)
                     /locus_tag="ACGI8A_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179292.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="ACGI8A_001290"
     CDS             5913..6641
                     /locus_tag="ACGI8A_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFH0179293.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6843..7334)
                     /locus_tag="ACGI8A_001291"
     CDS             complement(6843..7334)
                     /locus_tag="ACGI8A_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179294.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7345..7788)
                     /locus_tag="ACGI8A_001292"
     CDS             complement(7345..7788)
                     /locus_tag="ACGI8A_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179295.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7778..8617)
                     /locus_tag="ACGI8A_001293"
     CDS             complement(7778..8617)
                     /locus_tag="ACGI8A_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HFH0179296.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8836..10221)
                     /locus_tag="ACGI8A_001294"
     CDS             complement(8836..10221)
                     /locus_tag="ACGI8A_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0179297.1"
                     /translation="MENLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKYGFSIDELEGMDTAFAASYGEGRPVIAVLGEYDALDSLSQKVSTIKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10394..11137)
                     /locus_tag="ACGI8A_001295"
     CDS             complement(10394..11137)
                     /locus_tag="ACGI8A_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HFH0179298.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSKHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11157..12419)
                     /locus_tag="ACGI8A_001296"
     CDS             complement(11157..12419)
                     /locus_tag="ACGI8A_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0179299.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12603..13601)
                     /locus_tag="ACGI8A_001297"
     CDS             complement(12603..13601)
                     /locus_tag="ACGI8A_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0179300.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13585..15096)
                     /locus_tag="ACGI8A_001298"
     CDS             complement(13585..15096)
                     /locus_tag="ACGI8A_001298"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFH0179301.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15150..16112)
                     /locus_tag="ACGI8A_001299"
     CDS             complement(15150..16112)
                     /locus_tag="ACGI8A_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFH0179302.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="ACGI8A_001300"
     CDS             complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="ACGI8A_001300"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFH0179303.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17092..18114)
                     /locus_tag="ACGI8A_001301"
     CDS             complement(17092..18114)
                     /locus_tag="ACGI8A_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0179304.1"
                     /translation="MNFLSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKI
                     MKEKNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNT
                     DEDAKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETL
                     KGIVKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYN
                     PAYVFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVV
                     GFDDIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRD
                     STKESE"
     gene            complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="ACGI8A_001302"
     CDS             complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="ACGI8A_001302"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFH0179305.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19517..19780)
                     /locus_tag="ACGI8A_001303"
     CDS             complement(19517..19780)
                     /locus_tag="ACGI8A_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179306.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19850..20923)
                     /locus_tag="ACGI8A_001304"
     CDS             complement(19850..20923)
                     /locus_tag="ACGI8A_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFH0179307.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21171..21929)
                     /locus_tag="ACGI8A_001305"
     CDS             complement(21171..21929)
                     /locus_tag="ACGI8A_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFH0179308.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTIYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            complement(21926..22846)
                     /locus_tag="ACGI8A_001306"
     CDS             complement(21926..22846)
                     /locus_tag="ACGI8A_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0179309.1"
                     /translation="MMNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKT
                     TTMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCG
                     EITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDE
                     PTSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLS
                     EIKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTV
                     ISTLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22843..23043)
                     /locus_tag="ACGI8A_001307"
     CDS             complement(22843..23043)
                     /locus_tag="ACGI8A_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFH0179310.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23048..24142)
                     /locus_tag="ACGI8A_001308"
     CDS             complement(23048..24142)
                     /locus_tag="ACGI8A_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0179311.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24652..24792)
                     /locus_tag="ACGI8A_001309"
     CDS             complement(24652..24792)
                     /locus_tag="ACGI8A_001309"
                     /inference="COORDINATES: protein motif:HMM:NF021468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFH0179312.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            complement(25102..26409)
                     /locus_tag="ACGI8A_001310"
     CDS             complement(25102..26409)
                     /locus_tag="ACGI8A_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0179313.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26474..26953)
                     /locus_tag="ACGI8A_001311"
     CDS             complement(26474..26953)
                     /locus_tag="ACGI8A_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HFH0179314.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(27000..27863)
                     /locus_tag="ACGI8A_001312"
     CDS             complement(27000..27863)
                     /locus_tag="ACGI8A_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFH0179315.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27863..28618)
                     /locus_tag="ACGI8A_001313"
     CDS             complement(27863..28618)
                     /locus_tag="ACGI8A_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFH0179316.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28645..29133)
                     /locus_tag="ACGI8A_001314"
     CDS             complement(28645..29133)
                     /locus_tag="ACGI8A_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFH0179317.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29151..29552)
                     /locus_tag="ACGI8A_001315"
     CDS             complement(29151..29552)
                     /locus_tag="ACGI8A_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0179318.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29555..30052)
                     /locus_tag="ACGI8A_001316"
     CDS             complement(29555..30052)
                     /locus_tag="ACGI8A_001316"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0179319.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30076..30489)
                     /locus_tag="ACGI8A_001317"
     CDS             complement(30076..30489)
                     /locus_tag="ACGI8A_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0179320.1"
                     /translation="MKLVIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNI
                     EDLNKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLE
                     ALINREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30710..33277)
                     /locus_tag="ACGI8A_001318"
     CDS             complement(30710..33277)
                     /locus_tag="ACGI8A_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0179321.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33443..34186
                     /locus_tag="ACGI8A_001319"
     CDS             33443..34186
                     /locus_tag="ACGI8A_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HFH0179322.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            complement(34498..34704)
                     /locus_tag="ACGI8A_001320"
     CDS             complement(34498..34704)
                     /locus_tag="ACGI8A_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179323.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT        11242 a         6368 c         3552 g        13581 t
ORIGIN      
        1 cttaaaaata attcgtcaat acttttttaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaatgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatgcttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaagaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctcgaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgctaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggctctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcattcc taacaatgtt cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggta tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctatac aaggtatacc acaggctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtattgg tgggtcagaa
     4501 tagtatgata ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gccaaaaaat
     6301 ataatgtaac cgatttttta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gaatatatta aatttagctt tatgctttaa tgtatattca
     6781 tatatatcat ttacacttat ctaaactaca atttcattaa aattaaatat aaaaatcaac
     6841 tattattttt taaatagcat ctttcttaat ctattattgt aataacctat aaaagaactg
     6901 taaacatagt catatactat aaaggctatc tcaaatgaac ctataataaa tatgtttatt
     6961 ggaatataaa ctattgttct taataaaaaa tacaatataa gtatcattat atttgcaaat
     7021 actaatttca taaaatactc aatatatata gacctatctt tttctataag atattttaca
     7081 ataccatata ttccaaaggt aagtgaatat acaatccatt gagacttact tgccatcact
     7141 ataaatccta aaactataga acctatataa aaagcaattc cactcttaaa gccccattcc
     7201 attattacaa ttgatactaa aagtgatgct aaccccataa taaacaatgt atttacagga
     7261 ataatatttg taagagtcaa caatatcaca tttaatgcta ataaaactcc tccataagca
     7321 acttttatac tcatatttat catcctaaca acaagttatt aaatctccac caaaacattc
     7381 acaacatgaa tctaaacacc aaagcttagc acaattttca caacaatcgt cacctagaca
     7441 acaacagcaa tcatcacagc aacagcaaca acatccatct aaatcattac gtcttcttct
     7501 attataattg tcagccctat tgctataatc atttctatat tgtgtataac ttctaagtgc
     7561 atcactatat tcactatttt caggctccat aaattttgct ctttttaaga agtcttcacc
     7621 ttcttcataa tatcctatgt tcattgcaga aaagccagtt aaataatacc actctgcaca
     7681 tttgtcagtc aaagttttta aaaaatcata tgctttttta aattcactat tttctatgaa
     7741 tgctcttgct ctcataaaat tttcattatt ataagtatta ctgtacattc tttccacctc
     7801 tactttctaa tatattaata tatcttaaat aaacaccaga ataaattata ttttctatta
     7861 ttccaacatt tttatttaga tttagattat caattcccct agctaaaaac cctaatgatg
     7921 ttactattat tttttttact ttttctttta actcatcatt tttatcaata tactctataa
     7981 atggattata tcttcctttc ttaaaatctt catctaaatc ttcataggca tctagtagat
     8041 atatatactt acctatatta aaacctatca ttcttaagtt tctctcattt tcatcttttt
     8101 tatagacaaa tacttcaccc attatatttc caaatatact agatacttca tctatattta
     8161 tatttttatc tttttctaac ttatttaatt catttagttg ttgttttata tactctgctt
     8221 tttcaggata cttttcatat gctaatttta atttgttttt atatatataa tatgccaatt
     8281 tatcttttat gcgtttatca tccattaaat tatcttccaa tttataatac gttaaaagaa
     8341 tattcatact tgctgcatat tctgtaatat catttgtaat tacctttttc tttttaaatg
     8401 gatttactat acacacttcc tctgtactta tagactctgg gttatatact gctgttaata
     8461 aaacaattaa aaatgttata tcataattca aagaaaacct agagatttct ccatgatttc
     8521 tttttaaata tttacatagt ccacaatagt aagctttgta ataatcgtat tctctaaatg
     8581 ttaaatccat tttgtttatt ttaacatatc caaacataaa tactcctttt tgtactattt
     8641 tttatttaga ttatatatag tataacactt tttatcaaga ttatataatt tacagtattc
     8701 taagaaactt aaaaaattcc ttctaatcat tgtatcttaa atttaaaata attagaaggt
     8761 tgggttaaaa ataatattaa attatatact tatattaact catacaagtt agttgtgctt
     8821 atatcaattt aagttttaaa taattggtat atatttaaaa ctacctacag tatccttcaa
     8881 ttcttttttt gctttattta taaccaattc atcaagcatt acatcattta ttgaacatga
     8941 tatagattta gatgccaata acattgcttt aaatcccata tcagaacctg cactggcaca
     9001 agattgccat gtatgggcag aaataccaat aggccaaact gaacaagaac actgtgctgt
     9061 aggaattatg taactaacat ctccaatatc caatgataat gatatacagt tattatgagt
     9121 aaatgtatca tccgtaactt catcatgcag attcatctta gtaatatcct tatttccact
     9181 taatacacta gaaacactac ttcttttatc tatagacact gtctcagcta atttatctgc
     9241 aaaatagtag tcatcttcat tatagttttg tacacctact aatttcatat tttgtgatag
     9301 aatatctgtt aatattgtat ttggaatata gtcatatact ccacttataa tgtcatattc
     9361 cataatagta ccagtcatta atgctgctcc ttttgctata tcaacaatcc taccaaatac
     9421 atctcttaca tgctccattt taactcctct tatgtaatac cacacttctg caaaatcagg
     9481 aactatattt ggcatttttc caccattagt agttacatag tgtactctac aattatcaaa
     9541 tatatgttct ctcaaataat tacaacctat attcataagt tctactgcat caagtgcact
     9601 tcttccgtta taaggagctt gtgctgcatg ggcactaatc ccattatatc taaatttcac
     9661 agagtaatta gcaagtgttg ggattcttat aggagtattt atatcaaatg gatgccaact
     9721 aaaagcacaa tcaattccat caaaaaaacc tttttttatc atcaatactt ttccagataa
     9781 atcttcctct gctggacatc catagtattt tatagtccct ttcagctttt ttagtttgat
     9841 taattccttt actgcaatta cagctcctat tgaacctgta cccagtaagt tatgtccaca
     9901 tccatgacct gcaccaccat ctacaatagg ttctttaata gtggatactt tttgagacaa
     9961 actatctaaa gcatcatatt ctcccaaaac tgctataact ggtctacctt ctccatagct
    10021 agccgcaaat gctgtatcca taccttctaa ttcatctata ctaaaaccat atttttttaa
    10081 gtattctttt tgtaaatttg aagcataata ttctttatac tgaagttctg gatttttcca
    10141 tatcttatct gatatatctc ttaattctcc ctgtatctca tctatatatt ccatcaaaaa
    10201 ctttttattt aaattctcca tattaattat ataaacctcc ctgtttctaa taacttatcg
    10261 attatcagcc aaaatgacta attttaactg atttaaaaat attttctatc atacaaccat
    10321 tttatacaat aaaacctctt gacttgtaag tttcatttta tcaagtaaga ggttttcaag
    10381 ttttataaaa tttttagcat gttaaccaat caagcatatt aacccataat ttattatagt
    10441 atttccaatc agtaaattct tttggacccc agtgaggtgc acagtcagaa ctaaagatag
    10501 cagattttcc ttttccaaac tcgccaacag ctacaaatgg gtctcctcca atagttgcta
    10561 aaacaggaca attatctcta gctactgttt tgttatatcc taaaaacttt ggccattctg
    10621 ttggtatacc ttttaatact ggatgtttag agtcaataac ttctggaatt attcctgctg
    10681 gtttttcaac tctatcatct ttatctatca ttgttattgg taatacatct ttgatagcag
    10741 tttctccgaa tctagctttt gcatctattc ctgtaaatga catatatcca cctaccatga
    10801 ttaacgcccc accattattt acatactctt tgattagttc aagtctatct ggattactat
    10861 tgcaatctat aaaagtacta ttagataata agaacgtatt agaacctata tcagataata
    10921 ttatacagtc atactcattt aactcttcta atttatacgg aaaactatct gctgctacat
    10981 gtgcaggcat atgaactgtc tcatatcctc cagattctat tgcttctttc aaccacttta
    11041 cagcttcttc ataatgagtt gtaacaaacg tatcaaatcc ttttatatga gttatatttt
    11101 ttacccaaga ttcccctact attaatactt ttttcattta tatattcctc ctacttttat
    11161 aagtttaata ttagacttgc atacataagt atactatcta aatactcctt aatttcaaca
    11221 tattcatcaa ttgaatgaca ttgttctatt gaacctggcc ctaatataag tgttggtatt
    11281 tcagctaaat ttttcattaa tctcgcatca ttgccagctg ttgaaccttt tatctctaat
    11341 tttttcccta gagtttcttc tagtatttta tgtgcattca ctacaaaatc agaatccttg
    11401 tccatctcaa aacctaaccc tgattgatat atgtttatat ttggtttatt atctttaagc
    11461 cataaatctc cattagccct tgtcattatg acatttgtta catcatttac aacttgttca
    11521 taactcatta tatttggcaa gaagtgtaca cataaattaa atacacactt atctggcaca
    11581 gtagaacctg ctgttccacc atttattaca cctaaattta ttgtaggtga aggtaatagt
    11641 ggatgtttat atatcataag ccagttatgt tccaactctt ttatatcttg aagcaacaac
    11701 atagccttct caattgcatt tacaccctcc cacttactac cacagtgaag agacacacct
    11761 ttaacctcaa cttcaaagaa tacaaatccc atatgtgcaa ctattaagtt ttgttctgat
    11821 ggttcacaaa ttatgcaaca atctccatca ataccattca taactgcatt tatggtacca
    11881 tttccgccac cttcttcatc tactactgac attattttta cattaccagg tacatttaga
    11941 ccactatctt ttataagctt tacagctaat attgatgcta taagtccaga tttcatatca
    12001 gcagttccaa gtccataaag ttttccatta tcttctgtag cacgatatgg attatatttc
    12061 cacttactta tatcacctgg tggcatagtg tctacatgac cattaaatac aattgtctta
    12121 ccaggtaaat catcactata cttacaaatt aaattatatc tatcttgata attgtgacct
    12181 agatttcctt ctttatatat attttttgct tttttaatta attcttcgct catttcttgt
    12241 ctatctacac taaaacctat gtaatttgca agtttttcaa tatattcctg tccttctttt
    12301 tcaaatccac ctagtattcc atgaccaaca tcttcagtct ttatacttac aagttctttt
    12361 aaataatcta tgtattcttg ttttttacta tttaatgtat ctaagagaat ttttttcata
    12421 tttcctccta agatttattt ttacatttaa aatttcaagt ctaatctcat ctatttaagc
    12481 catagtaaac tttttcactt agcatattcc cattcaatgt ataaagatgt atgcaaatta
    12541 aagtataaaa atatatacaa aaacttaata atttttatag tttataagct aaaatttaat
    12601 tactatttag atttagctga tttttttaat tttatattat ttatccatat agcaaataac
    12661 attaccaaac cttttataat catttgatta tatgaagata cacttaataa gtttaatcca
    12721 tttcctaata ttcctattat tccgacacct atacatgtac ctactataga accagaacca
    12781 cccatcatat tagtaccacc aagcacaacg gctgctatag catccaattc tgctccatct
    12841 ccaacagttg gtgaacctga tgccaaacgt gatgatagta ttatcccagc taatgctgtt
    12901 agtagtccag ctagtgcgta tacttttact ataactttat ttacttttac tccacataat
    12961 ctagcggcct cttcatttcc acctactgca tgtacatgac gtccaaaatt agttcttttt
    13021 aatagaaaat gtcctgctat tacaactata atcataatta taactggtat tggtattggt
    13081 ccaatatatc ctctaccgag catcttaaat gcatctgtat ttactgatat aggtcttcct
    13141 cctgtaaata caaagcttgc cccacttatt atcgttaaca tggctaaagt tacaatgaat
    13201 ggaggtagtt tttgtgttga aataattgca ccttttatga ctcctaaaat tgtccctatt
    13261 gctaatgcaa gtatcatacc tacccatata ggtgcattat aattcactac tgttgtagcc
    13321 caaaatgtac ctactaaagc aattactgct cctacactta aatctattcc tcctgtaatt
    13381 attacaaatg tcatacctac tgcaagtatt gcatttattg atatttgtct tccaacatta
    13441 attaaattat ctaaagttaa aaagttagat gaagctacac tcattattat aaaaattatt
    13501 acaagtccta cagctgcact cccttggtct ctaaatacac ttgttaattt tgtcaataat
    13561 ggagaagatt tttcttcttt atgcttagtt attgaactca ttaattttac ctccgaaagc
    13621 caattttaat acattatttt caaccatttc ttcaccttct aaaaatccac ttatatgacc
    13681 ttctctcata accattattc tgtcactcat tgatattaat tctggtattt cagaagatat
    13741 cattattata cttacaccac tctccactaa atctcccatt agattgtata tctctgcctt
    13801 agcatttaca tctatacctc tggttggttc atccataatc aatatatctg gttgtaataa
    13861 aagccatctt gctattatag ctttttgttg gtttccacca cttaaacttt gtataacttg
    13921 attcatatgt ggtgttttta tctttaaagc ttctatgtat ttttctaaaa tctctttttc
    13981 cttttttcta tctataattg atttaaactt agatatcttt tttaacccag ccatagttgt
    14041 attttctttg acactcattt gtaaaaacaa tccagtacct tttctatctt ctgttattaa
    14101 tcctagtcca ttttctattg catctgatgg agttttaaac tttacttctt cattgtttaa
    14161 atatattttt ccagaatcat atgttctaag tccaaatata gcttctacta tttcacttct
    14221 cttacttcca actagtccac ctattcccaa tatttcacct tttcttatat taaagctaat
    14281 atcattaaaa acattgttct tagttagatt ttccactcgc ataatttctt cttttatttc
    14341 tacttttctt tttgggaaaa attggtctag ctttctatct atcattaaat taactaattc
    14401 ttcctcagac gatacatctt tggtatttaa agtcgaaatc gttttcccat ctcgcattat
    14461 agttattctg tcacaaatct caaagagttc ttccatccta tgggatatat atattatact
    14521 aactccactc tcttttaaat catttataag tctaaaaagt atttcagtct cttttttact
    14581 tattgaagaa gtaggttcat ccattattaa tactttagtt tctgatgata aaactcttgc
    14641 tatttctacc atttgttttt gagctatact aagctctttg actaaactat ttggatttat
    14701 atctattccc atattatcga aaatcttttt agcttcttca ttcatttttt tgtcattggt
    14761 taagactttg ccatttattt cttttcccat aaaaatattt tgtgctactg ttaattcgtc
    14821 aaaaactgtt agttcttgat atatggcatt tatacctatc tttttagaga tatctgggga
    14881 tatattctct atttctttgc cttcaaaaat aaattttcca gaagttttcg aatgtgcccc
    14941 agttaaaatc tttatcattg ttgatttacc agcaccattt tcacccagta gtgcatgaac
    15001 ttcgccatga aataactcaa aattaacatt atctaatgct ctgacaccag gaaattcttt
    15061 agcaatatta cttaacttca gaattatatt actcataatg acactccttt ttaaatagga
    15121 tttctttcaa ataatctcat tttattttac taattcttaa agtcttttat attttcctta
    15181 tcataaagta aagctggtac tggtatattc ttttctacct tttccccttt tatagctttt
    15241 actactgctt ctacagctaa acgtcccatc tctttaggtt gttgttgaat catagcaaga
    15301 atagttccat ctgttattcc acttgatgct tcttcagtta agtcaaatcc tacaatctta
    15361 acatccttat tttttgcaga ttcaagtgca gctttagctc ctagtacact attttcagct
    15421 gttgcatata ttatatctac atctggatta gattgaatta agttttctac tgtattaaga
    15481 gattcttctc tgtcatatcc tggttgtgaa tttaatatct taacatttgc tgaacctttt
    15541 aatgcatctt taaatccatc tattctctgc atttgtattt gagattttaa atctgtcact
    15601 attgctattt ctgactttcc acctaacttt tcatctatgt attttttagt gtattcccct
    15661 aattttttac cagcatcata actaactgtt cctacataac ttgtaatatt ttcagtattt
    15721 aatacagcat ctactgtaac tactggcatt ttttcaaatg ctccttctac tgctggtact
    15781 atagccgaat tatcagttcc aagtactatc attccatcta cattctgttg tataaaatct
    15841 tctattgctg atgtttgctt agctacatct tggtctgggt catttactat aactttcaca
    15901 cctaattctt tagctttttc ttttattccc tcatcaatat ctataaaaaa ttgatgttga
    15961 agagttattg tagatactcc aatagttaaa tctttcttgt ctgaaccacc tttattatct
    16021 gatttacttg gtccttccat tgaacaccct gttaacattc ctattgctag tattccagca
    16081 attcctaaag ataaaatttt tctcactctc ataattaacc tccattttta atagttttaa
    16141 atttgaacaa attagatttt tatttattat ttaaatactt ttcaacatct tctttatagg
    16201 gcataccaga ttgtgcacct agtttagtta cagaaagacc tgatactact actgcatatt
    16261 ctactgctgt atataaattt ttcccttctg ttatagccac tgccaatgct ccattaaatg
    16321 tatctcctgc tcctgtggta tcttgaacat caacacttat acaagataca tgcttgattt
    16381 cctcaccatc ataaaatcta gctccatttt ttccttctgt tattacaagt ttatttggat
    16441 atttttttaa tacttcttct atcccttcat ttgtgtcaaa aactatctta tattcatgtt
    16501 catttggtgt taagtaagtt actttttcta ttatatcttc atttagtttt actgctggtg
    16561 ctggatttaa taaaacccta atcctatttt caaaacagaa attcactaca taatttattg
    16621 tttttaatgg aatctctaat tgtagcaata ctatatctgc attctttatc tcttcttcat
    16681 tctttttaat taattctata tctactagct cattagtccc tggtacaaca acaatactat
    16741 tatcattttc acataatgtt ataacagcaa caccagaact tttgtgatta tttctggata
    16801 ctaagctata atcaacttta ttcgttctaa aatttcttat taactcctct cctaagccat
    16861 cttcccctat gcatgatatc atttttacat ttgctcctag tttagaagct gagatagctt
    16921 gattagcccc ttttcctcca ggtgatgtaa aaaaactatt acctaatact gtttctcctt
    16981 tttctggtct tttatctaca gaacatacca tgtccatgtt tacacttcct atgacaacta
    17041 tatttcccat tacattactc ctttacatat ctatttatgt ctctcgtgct gttattcact
    17101 ttcttttgtt gaatctctta tgaccaattc tggtttgaaa atcaattcat caaacgattc
    17161 ttttttatct cctcttaaac aatttatcaa tatttcactg gctttatatc ctatatcata
    17221 agacggctgt tttactgtgg taagagaagg gcttattaga cttgatgttt gaatatcatc
    17281 aaatcccacg acacttatat catttggtat acttaatcct ctttctttaa gacctcttat
    17341 gacacctatt gcaatcaaat catttgcaca aaatattgca tcaaaatcta tcttttctaa
    17401 attttttata aattcttgtc cccattcaat cttatatttt ccttcaaata cataagcagg
    17461 attgtagctt aaattacact cgattagagc ctttttatat ccttctaatc tatctactgc
    17521 tatttcattt tgaagtggac cacttaggta tattattttc ttatttccaa tatctaataa
    17581 atgttttgta gctatatagg ccccttcaaa gttatttatt ttaacaattc cttttaaggt
    17641 ttctttgttc atacttatct tcttatcaat caagacagtt ggtattttta tatctttata
    17701 atttgcttct ttccagtttg tatttgaaga tgctgcaaat ataattccat ctatcatcct
    17761 ctctgatagt gttcttattc cttcatattc ttttttagca tcttcatctg tattacataa
    17821 aattatattg taaccttctt cattagcttt gtcttctaca cctcttgata catctgtaaa
    17881 gaatggattt cgtatatctg gtataagaag ccctatagta ttagtttttt tagttactaa
    17941 actttgtgct aatttatttg gtacataatt tttttccttc attattttca aaattttttc
    18001 tcttgttgcc tgacttatat tttcatcttt attattaatg acttttgaca cagttgtttt
    18061 agaaacacca gccaattcgc caatttcttt tatagttata ctcaagaagt tcacccccaa
    18121 aatttaggaa accgttttac ttaaccggtt acctagatac tatcacatct tttttccatt
    18181 tgcaaccatt ttatataaat tttgatattt ttcccttttt tcaaaatatt gcaacttata
    18241 attcaatttt aatatttaaa ttaatctgca aaaaaaaatt aatatacttt tataattatc
    18301 taaaagtata ttaattaatt taagcagtat ttattaaagt tcaaatcctt gctctataat
    18361 cattttttta tcattttcaa tcccacttcc aacagttgtc aaataatcac caacaattgc
    18421 agaatttact cccccattaa atccaacttt gtcatatccc tttaatgata gccttccacc
    18481 agcatacctt atgtacacat taggaattat aaacctaaat actgctgttg tttttagtat
    18541 ttccattggt tctaatacat cataattttc cattggtgta ttcttaatag gatttaaaat
    18601 gtttattgga aatgatttta catttaattc tcgtatttca aaagccattt ttagtctctg
    18661 ttcttcactc tcattcattc caattatacc acctacacat acatctagcc cagctttttt
    18721 agcatctttt atagttttta tcctatcttt atatgtgtgt gttgtacata tattatgaaa
    18781 attatctcta cacgtctcaa cattgtggtg atatgtagtc acacctgctg acttcaacat
    18841 tttagcttgc tcataatcta taatacctaa agatgcacat aattttaaat tagtattttc
    18901 cctcaatcct tcatagatat ttaaaatatt attaaactcc tttcctgaca tacctttacc
    18961 acttgtaacc aatgagaatc tatgcactcc tttactctcc atctctttag ctctatttaa
    19021 gatttcattg taatctaata atgaatattc ttctacacca gttttataat gagatgattg
    19081 agcacaaaat ttacagtctt cactacattt gccagattta gcattcatta tcgtacataa
    19141 atctgctttt cttcccataa aatattctct tatttcattt gcagatttta atagaatatc
    19201 aaaatcgttt ttattatttg tatcaaggct tattaaatta agagcttctt cataacttat
    19261 ttctttttct ctcaatactt tattttttaa ttttattatg tatttttcca tatatcctcc
    19321 tatttgtcaa cttaatattt ttatgtagtt tacttattat atatagtatt gcatatataa
    19381 catttattgg tcaactttat tttagacaca agtttactat acacaaaaaa atactgcttc
    19441 aaaataatat tagtaaatgg attatacata ttattattaa aacagtattt tttattagaa
    19501 tataattctt actggtttat aaaactatcc ttgtcttaac tccatccaag ctttccattg
    19561 taataacctc taagccttca tattctctaa atagccattt taaatccttc atggcttgat
    19621 taacctgact ttttgttaga ttatttataa aatattcttt tctagtttcc tcatactctt
    19681 tgctagtcaa aaagtttctt agaaaattaa acgcaaatac acttggaata ttgtttacat
    19741 tagccttctt gctgttttcc ttctcatagt aaattttcat aaatgcatac tcctttgata
    19801 cttttagatt tctcttacaa taattatatg catttaagtg ctactattct tataatttta
    19861 ttaattcata ttctttactt cccataccca tttcacatgc atagtctatt attctagttc
    19921 catttacatg aggatgttca tgtttgaaaa catcatgacc catttcctta caaatcaaat
    19981 catagcatgc ttgttcaata gctacagggt ctgttgatgc taatattcct atatcatggg
    20041 caattggtct acctgaccaa ggtacacaat cacaaagtgg agttacattc ataacaaaag
    20101 ttatataccc taccttgtct tttttatttg atactgctcc ataagcgtat tctgccatct
    20161 tttcgacaaa cacatctgag tcactttccc attgtatagt tactgctcta gttggacaga
    20221 cagttggaca ttctccacat ccataacata catcagaatc tataactgct tttttatcca
    20281 ctatacttat agcttttgta ggacaagaat taacacactt tccacaacct acacacttct
    20341 tttcctttac tactggagta acatcagaat gttgcatttg ttttcctgct gcacttgcac
    20401 atcccatagc taagtttttt aatgctcctc caaaacctgc tgcttcatga cctttaaaat
    20461 gactcattac tatcattgca gaagaattat aaatttctcc acctattttt acactttcaa
    20521 aatgtttttt gtctattttt acattctcat aatttctact atataatcca tctgcaatta
    20581 ttactggtgc atttacaact gcataagcaa atccattttc tatagcagtt gtaagatggt
    20641 ctacagaatt tgttctactc cctgtataca gtgtatttgt atctgttaaa aatggctttc
    20701 cttcgcagtc tttaacctta tcaaccactt gtctaactgc cacaggactc atatatgtag
    20761 tatttccctt ttctccaaag tgaagtttaa ttgcaacttg gtcattcttt tctattaaat
    20821 ctttgaatcc tgcttcatca aataatcttc ttacattgtt tggtacgttt ttattttgcg
    20881 aatttgagta taaatcacaa aaatatactt ttgacttttc catatctaca cccctttaaa
    20941 atatttactt atactaattt tatcactttt aaagtaattt ttagtatatt ttaacataat
    21001 taaatattct tatatttatt tgatacaaat aatattattt gtattatttt ctacacttaa
    21061 ttctatatat taaaaatcaa aaagacttga aacttaagtc caagtctttt tgatttttag
    21121 gtgtagtttt taacaacata agattacttt accttgtgat aaaggtatta ttaaactggt
    21181 tttttattaa ataaaataac agctgctagt aaacatacta cagaaataag aactgttata
    21241 ataattggaa taataaattc tggcattgcc actttccctg aaatcaagtc tatatttttt
    21301 gacatcaaga aattaggagt ataaggtgca atctgtttag gaatagaaat taaactcaat
    21361 aaaagagttg ccaccaatac aagtaaaaca ctggcaaatg ctggtctaaa tagtacacac
    21421 ccaagaatta atacactaat atacataatt cctataagcc ataaattaaa tgctgaaaat
    21481 ataatatgat taagatttgc agttggccaa aaatatattg tatagccata agcaaataaa
    21541 aaactcaacc aaaaaccaat tgtcataata atcaccgtaa cagagaattt agacaatact
    21601 atagatgaac gtgataaacc tttagtcagc ataataacaa gtgtcccttt agaatattca
    21661 tttgataaaa tattacaaaa aataattaga gttaaactca taccaagacc agagatattg
    21721 ctaaaaaatt gtgtccaaga atctaaagca gttggtgcag cagtaacttt taaagtcggt
    21781 gcaaaatgag caatcaagtc tggcataaat tttgcagaaa taggactact tataccaaat
    21841 attagaaata aaataattaa actaaataat ttataatttc ttaaattctc tttaaattct
    21901 ttttttgtaa atgctatgta ttctctcact ttactacctc caaaaataaa ttttcaagtg
    21961 ataattctat tagttccatc tttactggac aaagattcat ttctgcgagt gtagatataa
    22021 ctgttttttg tattgctttt atatctttac catgcaaaac aatttccata ttagtttctt
    22081 ctgaatcttt tagtaaggat ttaataccat cactagactt aaacttttga atttcatcat
    22141 tacttgcaaa ctccagtaaa agtctatcct ttccgtgcat actttttatt tctgaaagtg
    22201 taccactaag ggcaatgctt cctttgttta atatagcaac atggtcacag attctttcaa
    22261 catctgatag tatatgtgtt gaaaatatta cagttgttga gtctttgatt ttaagcataa
    22321 tatctaatat ttcttttcga ccaacagggt caagtgcact tgttggttca tcacaaataa
    22381 gtaatttggg gcgtgaaagc aaagcttgag ctataccaag tcgttgcttc attccacgag
    22441 aaaacccacc tattcgtctc ttttcatttc caagacccac aagtgataac aactcttcac
    22501 tccttatctg tatttctttt tttgaaagtc ccgtaatttc accacaaaga gaaagatatt
    22561 caagtggtct catatagttg taaaactctg gaacatcagg cagataacct acatgacgat
    22621 tagaacttgt tttcccatag gtcactttct caccacaaac atctatagtt ccactatctg
    22681 gtttaagtaa cccaagtacc attttcattg ttgtagtctt acctgcacca ttcttaccta
    22741 caaacccaaa gactgaccct tctggtacag taaaatttaa attatctatt atctttcgat
    22801 taccaaaacc ttttgataat ccatttattg agacaatatt catcattctt cacctcttcc
    22861 tattgtaaaa tacaaaattg gtcctattag actaataaac agtactacaa taatccatat
    22921 tgccttattt ccaattttat aatttggatg tctcaataca tgaacaagtg cagtaattat
    22981 aagtattaaa tctaaaataa taacaggaac caaaagtggt aagtattcca tcaaattttc
    23041 cataatctta ttcctccaat aaagataatt tttctataag aaatttgtat tctttgtttt
    23101 cacttaatac agaaaacatt ggattatttt taatagcagc aacaatacct gcttttacga
    23161 ttgtatcatc acgtgtaata cctgttccaa tttcagaaat ccatctgtca agtttattaa
    23221 aaaagtcatc cccatgtatt ataattgggt atatatcacg tgttgctaaa tctacatatt
    23281 ttcgtaatga tttaatagct ccttcattat caccatgtat taaattaatt tgtgcctgtg
    23341 ttaaatatga agaaagcata atagcaggag ataatgtatc aagctcaaat atatctgaaa
    23401 ttgctagaat acgtctttct atttccttca tttgtaattt atctgcattt tgcaaaatac
    23461 ccactaggtt ttggactaat gatataaggt tctgatatgc accaagttga aatgtttctc
    23521 tcgcttcatc catttgacca ttcatcattt gcccctgtgc aagaagtata gattcattta
    23581 tcatgggaaa attgctgttt tgcaataaat caatcacttg tatgggttga tttaacaata
    23641 tataacaagt tgcctccata ctttttgctt gacggcaaat atctatgtca ttacaagttt
    23701 cctgtatacg aataaatatt tctaatgctt cactaattaa tagttcacgc tttttttcat
    23761 ctacaatatc atagtgatta actattaaaa gacccatccg aaaaatcaaa ggaaaacatg
    23821 aataatattt ttttatgatt tctcttattt catccatgac ttcatcaaat ggtttaaccg
    23881 taaagtcttt gcacagttta ttatacagtt tattaatatc ttccttcatc atttgaggtt
    23941 cataacatat taactcatca acggtaatgt tgaaatatgt cgcgagttgc ggtaaaaaag
    24001 ttatgtcagg atagctttgt ccagtttccc atttagatac agaagcttta gaaacgccta
    24061 tataatttgc aagttcatct tgtgtaattc ctttttcttt acgctttaaa atcagcgttt
    24121 ttgcgatgtt taattcattc atgtcaatcc ctccttatgt ataattatat ccttaaacat
    24181 gtaaagctaa caagcgattt gaagttgact tttgacgaaa aaatcaacca cagagaaaca
    24241 aataaacaag tatataaaat atatatttaa atttatcttt aatctctttt gtaaaatatg
    24301 taaactttgt aaataaaatc catattatat aaaactaata gttaatctat ctaaatacag
    24361 tttttattta tcacgttttt aaatattttg tgtaaaacct cttttaatat aaacacctta
    24421 ttttcatttc tcatttcata ttctttttaa aatactgaat ataactcttc gattttccat
    24481 ttaattcttt tataaagtta ttattcgtat ttatttctct aattataaca ggcaactatc
    24541 atgtattggt atttgtctat tatataaaaa tctataaaag aaaagcagtc aatcatttag
    24601 attaactgct tgtacatata tcttattaaa ctgtataaac tataattttt attattctcc
    24661 attttcatcc caagtcataa atccccatcc actatctgga taagtatcaa tagacttttc
    24721 tctaatcata taaccaactt tataacactc ttttgttatt ttattagata gtatacatcc
    24781 ttctccattt atctaattcc attcaattaa cttttctttt caaccttaac ttttataaaa
    24841 tcatcttttt taataatttc atacttattt tctcttcaac atcatcaaat gagggtgtct
    24901 caaaattaac tttttacttc ataaggcact cttttttgta tgaagtaaaa tatattttca
    24961 tcatttcaac aataagtaag aggcttatct ctattttgag acagccccat ttgatggtgt
    25021 tgaacattta aataaggttg aactcgtttt atctctcttt ttaagatatc tgtaaatttg
    25081 cacataaagt ctcttcttct tttacaattc taatagtttt attaaagcca aagtatatat
    25141 ctccatattc tttcgtatat gatttattgg tactctttca tctactccat gaccaccagt
    25201 tttatacgct aagaacttag gtccaaacgc caccatatct ttaaaccaac ttgcatatgt
    25261 gccaccaccc atagcaattg tatatccatt ttcatctgtt acttcttcat aggcttctaa
    25321 taatttttta agtactaaat tactctcatc cacataatgt aagtctttat gagcaataac
    25381 ctctattttc caaccattac aaaaaacctt tttgaatcta tctattatat tgtttaaaac
    25441 tatattttta ccatacctta tatctaactc acatacgatt ttttcctcat ttacttttaa
    25501 tattcctaaa tttaaggtag tgtttttgaa tacctcatca tattgattaa tccctaatgc
    25561 atctccataa taatcactac aaaggtattt tgaaactaat tcgatatctt ctctatgctg
    25621 aaaatccata ttatcctcaa ctatctttat taccatttta gatatagcat tttctccctt
    25681 ttcaggatta cttgcatgta cagctactcc tttagtagtt aactctgaaa taattccatt
    25741 aataattttt gcagtacaat gagatggaac aacattttct ttagttcctc cccttactat
    25801 tatgtaaggt ttacttttgt caattttaat ttctttttct atccttatat gaacccttcc
    25861 tttttctcca ttaaccactg gatacatagc atctggagta aatcccatac ttggaatttc
    25921 ttcaccttta tctaagtaat atttgataca tttcatacct gtctcctcat ttgtaccaaa
    25981 aattaatctt attcttttat taattgtagg atacatatcc tctatgtttt tcaatacatg
    26041 tataagcata gatacagcac ccttattatc tattacacct cttccataaa taatattgtc
    26101 aattactgct cccgaaaatg ggtctacact ccacccctct cctggtggaa ctatatctaa
    26161 atgcattgga atccctatta attcctctcc ctctccaatt tctgcatatc caatataatt
    26221 atcaatattt ttaacactaa aacctaaact agcacagtaa tctaaagttt tatctaaaga
    26281 ttttttaggt cctataccaa aaggacattg tgaaataggt tcaccttgtt gtgaatcaat
    26341 ttttatcaat tcttttagaa attctatggt tctatttaaa ctattatcta cacttctgct
    26401 tatatccata aatcaaccta ctttctatct ttttaaagcc tgtccttaaa gaacaggctt
    26461 ttagcattaa aaattatttt tttgtaaata ctaattcaca aacttcatca tcatgagcac
    26521 atttttgagc aaaatctaat ttaacacctt gtgcgcagtc aactcttcca taatccccat
    26581 aacaagctaa tctacaaatt tcttttcttt cttctttagt taatccatat tctttccaag
    26641 cagcatctaa tggacagtga tggaatttta aaacgccatt ctcatctgta ttttctacta
    26701 attccatttc aaacactttt tgtcctttac tgttatataa catttctgcc atttttcctg
    26761 gagtatctgc tacgttatat ttttgacctc tcattttacc aaatccatat attgcttctt
    26821 tacatatatc atctggatta gctcctaatt tttcagcttc tttcataaga agatagaacc
    26881 atactgctct ttctcctgtt gctcctctaa ttccttctaa atattccttt tctgtataca
    26941 ttttttctga catttctttc tcctcctaaa attaaattct atattttaat cactgtgaat
    27001 tagaatattc caatgaatga ccctaccaat gctaatacaa ctaaaattac taataactta
    27061 gttattgtcc acttcttgta tttcattccc caatatacga agaatacaac accaagtggt
    27121 aataaacctg gagcaatcat atttaatgta tcttgaacaa caattgcttt accaccaata
    27181 tcaaatgata gcggagttga aagcttaact gtagttgcag ataatgctcc catcataaac
    27241 attcctagaa ttgaagcacc atcaattatc ttattgacaa taccacctga tagaattttc
    27301 ttaattgaat ctctacctaa agaatatcct aaatgaaata agtaataacc ttcgcaaaat
    27361 agtaatatac taaacattat tgggaaaaat gcacctataa cacttccttc tgctccaaat
    27421 gaagcaccta aagctaagaa tattgtttga aatgtaccaa aatctaaagt atctccaatt
    27481 ccagccaaag gtcccataag accattctta acacctgaaa taacttctcc tgggattttt
    27541 ccttcagtag ctttctcttc ttccatagct aaaactgtac cgtgaattaa tcctccccat
    27601 atagcttgag tattaaaaaa ttgtaagtgt ctctttaacg catctactaa atcttctttt
    27661 ctcttatata acttttctaa agccggagtg aaacttgcac aaactgctaa tgcttgcatt
    27721 ctttcaaaag agttggataa ttctgctgtc caccaccatc ttagatatgt ttttgttaca
    27781 tctttctttg taagtactct tgtcttatta acttcagttg gaccttgtcc atttttaact
    27841 tctttatttt ttatttcttg cattatactt cacctaccct tttgtcttct ttcataaagg
    27901 taattaaaag agctatacaa acaccaaata ttgcagctgc agtaattgat atttcaaaat
    27961 attgaaccat aaaaaatcct ataataaaca ttggtaaaaa tttattttta cctatagtaa
    28021 atattgttgt tgcaaaacct aatgcaggta atataccgcc cattactgtt aatccattca
    28081 ttaaccattg tggtataaca tctaataatg attgaactaa atctgctcca aaaaagttac
    28141 atacaaaaac tattggaaac cttaaaacaa acccaaatat taaagaataa agtgttgcac
    28201 atctccagat gccttttgta ttaccttctt cagcatactt atctgcctta tgaaccaata
    28261 cagcattacc agttcttctt aagttattaa ctaatactcc tataacacct aaaggtaccg
    28321 caatagatac tgcaacttct gcattaacac cagtttgaag tgctactggt attgcaataa
    28381 gagcagctag acatttatct gatggaatat ttccccctgc tgcgaccatt cctagataaa
    28441 ccatttctat acttgcacca acaatcatac cagttgttac atcaccccaa agtaatccaa
    28501 ggctaaaacc tataaataaa ggagacttta gtgttgagaa aagggtatac cctgcaagac
    28561 ctgctgcaaa ccaataccat aatcctgtta caattgccaa catcaaaaca gattccattt
    28621 ataattcctc ctaatttata ttttttattt aaatttcttt aacgcatttt catatgtcat
    28681 agatgattcc tctggtataa cttgtaggac tatttcaacc ccattttcat taagttcatt
    28741 aagttgttct acatcctcat ttcctaatga aacagctgta aacaccattt tctttcctgc
    28801 ttcatttgga atacctccta attgaatctt ttcatattta agtccagctt tataagcctt
    28861 atttgccata tcaacatttt taaatagaat aaatatattt ttatctccta aagtgttgtt
    28921 ttggaatgct tgtcccgcat cttctgctgt taaaataact acttctactc ctgatggtgc
    28981 tgccatttta tatatgtcag ccataaattc atcttgacct aagaagtcat caactattat
    29041 tattttttga gcttgagcga tttttaccca ttttgttata acttgcccat gaattaatct
    29101 gtggtctact cttactaatg ttatttttgc cattatattc acctcataat ttatattttt
    29161 ttaaattctt taattaaatc cacacaaagg ttattatttt ttttgacgat tctttctggt
    29221 aatgtatgta atttatcttc tttgcttaat tcttcatcta cgattaaaat tgtctgtaag
    29281 ctaagtccat ttattgcatc tattcctctt cttatagcaa cagctattgc aacacttgat
    29341 gtagatcctc catataaatc tgatattata attttcacat cttctttatc tactacttct
    29401 tcaacctctt tcataaatac agaaacatct ttttcagaac ttagtgataa aacttcatat
    29461 tgatttgaca atccaaagtt tttatttgtt tcttctacta agacttttcc ccaagttcca
    29521 tggcacagta tgattacctt atatttattc attgctaaat aaactctcct ttatagcgat
    29581 taatattttg gtttaattct acttcttctt catcaggtac catttgaatt gatacattaa
    29641 tattcttttt aataaattca tctattaaaa gaaactcttt tttattaatg tatacatttt
    29701 gagatatttt cttttttcct attcctgtag gtattcttcc aacaataagt tttttgatat
    29761 taatcccttc ttctatcatt ctatttgcag tatctaaatt tttaattaat acaaaaacgt
    29821 tatcatcagc ttgattatta taaaaatatc tccttgattc acttacagtt aatattgata
    29881 aatcaatatt ctttggtgct gccattttta tcaaaccttt agcaataaca tcattggttg
    29941 tttcatcatc tactactact atctttttta cacctagatg cttaacccat ttatatataa
    30001 cttgaccatg tattaatcta tcatcacatc tgcaatattt tactaaagac atttttaatc
    30061 aaactccttt ttctattaca ttgagctaag aactttgtta atattgttta taccagtttg
    30121 tcctgcttca atacatgctt ctgctaactc ttctaaactt tttccctctc tattaattaa
    30181 agcttcaagt agcattggaa gatttactcc agtaagtgca tgaaacttgc cacctttttt
    30241 aaggtttaaa cttgcaacat tacttggcga acctcctaat aggtcagcta atactaaaac
    30301 tccgtcacct tcatctaatt catttaactt ttctacaaaa tttttattta agtcttcaat
    30361 attatcattc ctatttagtt taattgctgc tgttctttct gctggtcctg caataagttc
    30421 acagcttttt ataatttctt cactaaaact cccatgagtc ataactatta ttcctatcac
    30481 caatttcacc tcacttttct tgtcactttt tatataagca attgccgtgc caacttatgt
    30541 gtatcaataa caaatggcta ttttacagga tttaatcaaa actttttatt caattttttt
    30601 taaaaactta ttatttcgat atgtatcaaa agattttttc aacctgtatc aaatctgtat
    30661 caaaaaaagt aaaaaaaaga tagccttttg gctattttta aattttgtat caacttttga
    30721 atttatctgt atcatattgt gattcaaata tatctaatat atttgcatat tccatatctg
    30781 aaataattat atcgaatgtc tcttctacta gcttaaaact ttcttttata actaaatata
    30841 attctttatt tctactaatt ctttcttcat atgatttata aactaaagac tcttttatta
    30901 ttactctttc aatcatacat gaacaatgaa atataaactt tactaaaagt tcatcttgaa
    30961 ttactaaaga atagtgttca ctaattctat taattacttc ttttaatata aaataagcct
    31021 tttccccatt taaaaaagtc aaagttttac taatattatc aattagtaat ctatcaaaat
    31081 atctaccacc attatctttt tcagcatatg taacttccat atctccataa tgtttcttta
    31141 tagattctat aatattaaaa tataatttct ctaatgtcat ttctggagtt agagcctttc
    31201 tagttgcttc tattacaata ggtgtactta ccatatctat tgcttttact tcttttcctg
    31261 tagcatcacg aataacttta gcaaatgtta aaatagaccc catatcagtt aaaattaaaa
    31321 ttccctttcc tttatcaatt ttattggcta tttctacagc ttgtactaaa atatcctgaa
    31381 ctctatcttc taatcccata ttaattgcca ctgcatggtt acaatccagt aaagtattgg
    31441 ctacattcac catgctagat gctgtagatt caccatgcat tatgactaat attgctatac
    31501 tctcctcatt taatcccaca tacgataaat aaagaaatgt tgctaaaaaa attgcttcat
    31561 cttctggaaa ctctactcca tacattaatg acatgttaga taatatttct tttgctatgt
    31621 tatattcata tgctctttcc ttcataattt catctttatt aggatgcttt actatatttc
    31681 caatctttat tctttcaatt aaattctgaa tatgtaaagc tatagctttt attaatttat
    31741 ctaatccttc tcttttgctc catttatcat gcttttgtaa aatatatttt acagtagttg
    31801 caattgaatc attaacaatg ttgttataag ctgtttgatt actactatta tatataaaag
    31861 tattcattaa attataggaa tacttctgta tgtctccatc tataacactt ctaatttgtg
    31921 actctttaat accttcttgt tgaagtttat tccaagtttc ttttatcatt ccataaaaat
    31981 ctaaatcata ttttttattt tctattaata aattttcact ttcaaaatcc ttttcttctg
    32041 gtaaaaaaat aatatcatct tttccaataa aactgaaatt ttgtacaaac tcctcttttc
    32101 tattatttcc atataatgat tctctaatat tatttggtag aagagagagc cttacatata
    32161 cacattccct tttataggtc atataatcta aaaaagctct tgcacaaatt aattgtatat
    32221 cgctttttaa ttggcctatg tttcccttac acttatataa aataaatgcc ttaataactt
    32281 cttttgaaac ctttaactta acaccaatcc taacactctc ttctttaaaa aaataacata
    32341 taagcttcat tctctcttga aagcctcttt cctctaaaga cggaatcttt atagttacag
    32401 gtattcttct taaaaacgtt tccaacattg ctgtttctgg gtcttctgta gtggctgcaa
    32461 ttatcataac tctacactcg tgtaccttat tagcctctcc taaccttcta tatatccctt
    32521 tatccataag cagaaatagc atttcttgtc cctctgcaga taatctatga acttcatcta
    32581 aaaacaatac tccattatta gcttcatgta caataccata cttatcttta tcagctccag
    32641 taaatgctcc ttttacatat ccaaaaagct gcgctaataa taattgctga ttatcagaat
    32701 aatcagcaca gttaaatatg ataaatggtg cattatcact atcttctttt atagttaaac
    32761 tatacttata catatattct gcaaatatag atttaccaac tcctgtaggt cctattatta
    32821 atgtactcaa ccccttaggt ggatatatta ctgctgcttt agcctgttct atttgggatc
    32881 ttaaacttcc atcacatcct actaaattaa aaatattgtt ctcttttttt atactctcta
    32941 aaatatactc atctttgata gaatcatttt cactatattc gtttatagag ttaatattat
    33001 caaatatccc tttatttagt ttctttccta aaatactctc aatactttct ttatataagt
    33061 actgaacagg cttacccttt atctttataa tgatactttc ttttattaat tgatttaatt
    33121 ccttactagc attacttcta tttataccta cttcagtagc tatatattct gcatcaaatc
    33181 caaatctaat gccattatta aagttcaagt gttcttctgt taaccttttc aagcaaatta
    33241 aaactttttc tttccttgat agcttctttt gtgtcatagt taacctcccc tataagcagt
    33301 aatactgtta aacatttatt attaagctta attctacaat attttaatat ttttgtctaa
    33361 ttatgtaact attgattttc tttcctattc gttttataat ataaattacg ttaccctcat
    33421 cttttataga ggaggaattt atatgaattt tttatcagat ttacacaccc actctatagt
    33481 cagtggtcat ggctatagca ctttactaga aaatattaat tattgtaaag aaaatggaat
    33541 taagatacta ggtacatctg aacatggtcc taagatgcct ggttctcccc acagatggta
    33601 ctttcacaac attcaaaata tcccaagaat cataaataac attataattt taagaggctg
    33661 tgaagcaaat atactagata ttaaaggaaa catagaccta gagccttttg taattcctcg
    33721 gttagattat cttatcttgt catttcacga agctgtgttt tctccaaata cattagagaa
    33781 taatactaaa gctttaataa atgcaataaa taagcatgat aatattgaaa tattaggaca
    33841 ccttggaaac ccaaactacc caatcgacta tgaattaatc attaaattag ctatagaaaa
    33901 gaatatctta atagaaataa ataattgttc tattaaaggc gtgtcaagaa atggtagctc
    33961 ggataactgt aagtatattg ctacactttg caaaaaatat ggtgctaaaa tcatactaac
    34021 ttcagatgct cacatttgtt ttgacatagg taactatgag tattctgaaa atatcttaaa
    34081 ggaaattaat tttccagatg aattaataat gaattatcct aaaaaactta ttaatcattt
    34141 tcatcaaaaa ggtaaattac tagatgttga ttatacaaat atctaagtaa taatacatgc
    34201 ttactttaac tcccataaaa attttaatac tatttatatt atatgaaata aagaaattat
    34261 aataaagaag ctatttaata aagcttaatt acttttatga acttgatgga ttgatatggt
    34321 ctatgatagc aatagaaatt ataacatttt taataagctt agttttatat accttatagt
    34381 ataaagctaa aaaaaagaat catctaaaat aaagtaaatc ttatataagg tttactttat
    34441 ttttatttgt aataatcatc aatacaaatt attttattat atgtaattat ttatgtctta
    34501 accaatttta tcgaaaataa cagtcttctc ttttaaagag atttttccta cttgatatcc
    34561 atattcattt aattcctttt tatatttcaa aaacgaatgg tctatctcta tcccagcaat
    34621 atcttttatt tcttcaaaag ttaatttaaa cgattgacta ttgtttttct taacatattc
    34681 ccataaagaa ctatatttac tcatcttata tcactccttt taaaatcata tcttatctat
    34741 taa
//
LOCUS       DAYATN010000028        33533 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.028, whole genome shotgun sequence.
ACCESSION   DAYATN010000028 DAYATN010000000
VERSION     DAYATN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.028"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            42..1328
                     /locus_tag="ACGI8A_001321"
     CDS             42..1328
                     /locus_tag="ACGI8A_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFH0179324.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1440..1913
                     /gene="grdA"
                     /locus_tag="ACGI8A_001322"
     CDS             1440..1913
                     /gene="grdA"
                     /locus_tag="ACGI8A_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_110943085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1572..1574,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HFH0179325.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            1945..3255
                     /gene="grdB"
                     /locus_tag="ACGI8A_001323"
     CDS             1945..3255
                     /gene="grdB"
                     /locus_tag="ACGI8A_001323"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2992..2994,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HFH0179326.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3511..5043
                     /gene="grdC"
                     /locus_tag="ACGI8A_001324"
     CDS             3511..5043
                     /gene="grdC"
                     /locus_tag="ACGI8A_001324"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HFH0179327.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5054..6199
                     /gene="grdD"
                     /locus_tag="ACGI8A_001325"
     CDS             5054..6199
                     /gene="grdD"
                     /locus_tag="ACGI8A_001325"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HFH0179328.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6509..7588
                     /locus_tag="ACGI8A_001326"
     CDS             6509..7588
                     /locus_tag="ACGI8A_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFH0179329.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7701..8300
                     /locus_tag="ACGI8A_001327"
     CDS             7701..8300
                     /locus_tag="ACGI8A_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179330.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8486..9358)
                     /locus_tag="ACGI8A_001328"
     CDS             complement(8486..9358)
                     /locus_tag="ACGI8A_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0179331.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            9775..11178
                     /locus_tag="ACGI8A_001329"
     CDS             9775..11178
                     /locus_tag="ACGI8A_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179332.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            11205..12749
                     /locus_tag="ACGI8A_001330"
     CDS             11205..12749
                     /locus_tag="ACGI8A_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HFH0179333.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            12780..14171
                     /locus_tag="ACGI8A_001331"
     CDS             12780..14171
                     /locus_tag="ACGI8A_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFH0179334.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            14364..15860
                     /gene="abfD"
                     /locus_tag="ACGI8A_001332"
     CDS             14364..15860
                     /gene="abfD"
                     /locus_tag="ACGI8A_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HFH0179335.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            15995..16270
                     /locus_tag="ACGI8A_001333"
     CDS             15995..16270
                     /locus_tag="ACGI8A_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179336.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            16294..17601
                     /locus_tag="ACGI8A_001334"
     CDS             16294..17601
                     /locus_tag="ACGI8A_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HFH0179337.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            17621..18739
                     /locus_tag="ACGI8A_001335"
     CDS             17621..18739
                     /locus_tag="ACGI8A_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HFH0179338.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            19026..19946
                     /locus_tag="ACGI8A_001336"
     CDS             19026..19946
                     /locus_tag="ACGI8A_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFH0179339.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            20061..21218
                     /locus_tag="ACGI8A_001337"
     CDS             20061..21218
                     /locus_tag="ACGI8A_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFH0179340.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(21303..22802)
                     /locus_tag="ACGI8A_001338"
     CDS             complement(21303..22802)
                     /locus_tag="ACGI8A_001338"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFH0179341.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            23398..24822
                     /locus_tag="ACGI8A_001339"
     CDS             23398..24822
                     /locus_tag="ACGI8A_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFH0179342.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            24848..26986
                     /locus_tag="ACGI8A_001340"
     CDS             24848..26986
                     /locus_tag="ACGI8A_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0179343.1"
                     /translation="MDNLKKKKITSRQKKIILMIVANSKKNIPITISEIAGTLELSSR
                     TVLRDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEER
                     NLIILSKLLVSNEPVKSYYFAKILKVSEWVLNNDFALASKWLERFDIELVRKPGLGVY
                     LKGQEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCI
                     IRKVEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITE
                     EFKLAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITN
                     KMIDLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYD
                     VVEKISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSR
                     FLSTKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSL
                     DDEQIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEK
                     FESKDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERID
                     IMKLSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKI
                     NKFSIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            27016..27438
                     /locus_tag="ACGI8A_001341"
     CDS             27016..27438
                     /locus_tag="ACGI8A_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0179344.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            27457..28608
                     /locus_tag="ACGI8A_001342"
     CDS             27457..28608
                     /locus_tag="ACGI8A_001342"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFH0179345.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      28714..28815
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            28998..29570
                     /locus_tag="ACGI8A_001343"
     CDS             28998..29570
                     /locus_tag="ACGI8A_001343"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFH0179346.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            29634..30284
                     /gene="fsa"
                     /locus_tag="ACGI8A_001344"
     CDS             29634..30284
                     /gene="fsa"
                     /locus_tag="ACGI8A_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFH0179347.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            30436..33360
                     /locus_tag="ACGI8A_001345"
     CDS             30436..33360
                     /locus_tag="ACGI8A_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0179348.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVMKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT        13163 a         3749 c         6318 g        10303 t
ORIGIN      
        1 tcaaatgcat atacacaaat taaataacag gaggtgcaag tatgcgtctt gaattaggga
       61 aaatctttat aaaagatatt caatttggtg atgtaacaga agtaaaagat ggagtattat
      121 tcatcaataa gcaagagatg ctacaagaaa ttggtggaga tgagcatatt aaatctatag
      181 atatagaatt agctcgtcca ggtgaaagcg taagaataac acctgtaaaa gatgttattg
      241 agccaagagt aaaagttgaa ggtaatgggg gaatcttccc tggtatcatg tctaaagttg
      301 acactgtagg agaaggaaaa actcatgctt taaaaggtgt agctgttgta actactggaa
      361 aaatagtagg tttccaagaa ggtataatag acatgactgg agaaggagct aagtacactc
      421 cattttctaa attaaataat gtagtagtaa tagctgaacc tattgatggt ttaaaacaat
      481 acgctcatga aaaagctgtt agaatgatag gattcaaagc agcaatgtac ttaggagaag
      541 tagcaagaaa tcttactcca gatgaagttt cagtatatga aactaaacct cttcttgaat
      601 caataaaaga atatccagag cttccaaaag taggatatgt ttacatgctt cagactcaag
      661 gtttattaca tgatacttat gtatatggag tagatgcaaa acaaatagta ccaactttat
      721 tatatccaac agaattaatg gacggagcaa ttgtaagtgg taactgtgtt tctgcatgtg
      781 acaaaaaccc aagttatgtt catataaaca atggggttgt tgaagactta tatgcaagac
      841 atggtaaaga tataaacttt gtaggtgtaa ttataactaa tgaaaatgtt tatctagcag
      901 ataaagaaag atcttcaaac tggacttcta aattatgtaa atatttagga ttagatgcag
      961 ttatagtttc acaagaaggt tttggaaatc ctgatactga ccttataatg aactgtaaga
     1021 agatagaaat gcaaggtgtt aaaacagtta tagttacaga tgaatatgct ggacgtgatg
     1081 gaggttctca atcacttgca gatgcagatg taagagctga tgctgttgta acaggtggaa
     1141 atgccaacca agttgtagtt cttcctaagt tagataaagt tattggacat ttagaagtag
     1201 tagatgtaat agctggtggt tctgatggaa gtttaagagc tgatggaact attgaagttg
     1261 aaatccaagc tataacaggt gcaactaatg aaacaggatt tggtcattta actgctaaag
     1321 gatattaaga agtattttat taaacgattg agtaatgtta ataaaacata ttatagataa
     1381 ttataataag tgttatagat atataaaaat atattagaaa tataataagg aggattatta
     1441 tgagtttact tagtaataaa aaggttctta taataggtga ccgtgatggt ataccaggac
     1501 ctgcgataga agaatgtgta aaaacagtag aaggagcaga ggttgttttc tcatctacag
     1561 aatgctttgt ctgaacagct gctggggcta tggacttaga aaatcaaaac agagttaaag
     1621 atgctgctga taaattcgga gctgaaaatg ttgtgatttt actaggtgct gctgaagccg
     1681 aagctgcagg tcttgcagcc gaaacagtaa ctgctggaga tccaactttc gctggaccac
     1741 ttgctggagt tgccttagga ttaagtgttt accacgttgt tgaggaacca ataaaatcat
     1801 tatttgatga aagtgtatat gaggaccaaa taagtatgat ggaaatggtt ttagaagttg
     1861 aagaaataga agaagaaatg tctggtataa gagaagaatt ttgtaaattt taatgcctaa
     1921 aagcaaaagg ggtgaaacta agagatggga aaactaaaag cagtacatta tattaatcag
     1981 ttctttgctg gtataggtgg agaagaaaaa gctgatacta agccacatgt ggcagaaact
     2041 ttacctccaa taagtttaca acttgataaa ttattaggtg aagatattga aatagttgga
     2101 acagtagttt gtggagatag ctatttcaat gaaaatatag atagtgcaag tgaagaagtt
     2161 ttatcaatgg taaaaggttt tgaaccacaa cttttcatag ctggtcctgc atttaatgct
     2221 ggtagatatg gggttgctgc tggaacaata actaaagttg taaaagatgc tttgaatata
     2281 ccagctttaa caggtatgta tgttgaaaat ccaggagcag atatgttcaa aaaggatgtt
     2341 tatgtagtag aaacttctga ttctgcagca ggtatgagaa aggcacttcc taagatagct
     2401 aaacttgcag taaaacttgc taatggagaa gaaattggaa ctcctaaaga tgaaggatac
     2461 atagctagag gaataagagt taactacttc catgaagaca gaggttctaa gagagctgtt
     2521 gacatgttag ttaagaaaat aaaaggtgaa ccatttgaaa ctgaataccc aatgccaaac
     2581 tttgatagag ttgacccaag taaagctgta aaagatttat caaaatgtaa aatagcttta
     2641 gttacttctg gtggtatagt tccaaaagga aatccagata gaatagagtc ttcttcagct
     2701 tctaaatatg gaacttattc tatagcagga gttatggatt taacagaaga gacttatgaa
     2761 actgcacatg gtggatatga cccagtgtat gcaaatttag atgcagatag agtattacct
     2821 gttgatgttc ttagagattt agaaaaagaa ggtgtaattg gaaaattaca tgagactttc
     2881 tatacaactg taggtaatgg tacatctgtt gcaaactcta aaaaatatgc ttctgaaatt
     2941 ggagcagcat tagtggctga tggtgtagat gcagtaatat taacttctac atgaggtact
     3001 tgtactcgtt gcggtgcaac gatggtaaaa gaaatagaaa aaactggact tccagtagtt
     3061 catatgtgta cagtagtacc tatttcatta acagtagggg ctaatagaat agttccaact
     3121 atagcaatac ctcatccact tggaaatcca gcgcttgacc ctacagaaga aaaagcccta
     3181 agaagaggcc ttgtagaaaa agcattaaat gctttaacta cagaagtaga tggtcaaaca
     3241 gtatttgaaa aataaaaata acacacacat atattatata gataaaaata taaacacaca
     3301 ctaaaaataa aaaataacta tacacaaaaa aataatatag atttcatgaa tggataaatt
     3361 ggagttattc attcatgaaa tcttaaaaaa tgccaaaata cataaacaat aatagaatca
     3421 aatatacaat aaaatacaat gattagagct tttaataagt ttaataagat taaaaaataa
     3481 gtgttcataa aatttatgga ggtgcacaaa atgacttatc cagttttaaa aggggcaggc
     3541 tatgtactta ttcatactcc agatatgata gtgcaaaatg gaagtacttg tacagttgaa
     3601 agagcaacaa atccagattc agaattttta aaagaagtaa gtaatcatat aagaagttat
     3661 gaagatgtag ttaactacat gccaaatcaa gtttatatag gaaatagaag accagaagaa
     3721 ttaagagatt taccaatgcc atggtgtgaa caaaagattg agggaactag aaatggtaag
     3781 tttggagaaa taatgcctca agatgagttc atagctttaa tgcaaataag tgatgcattt
     3841 gatttagtaa aattatcaca agaatttatt gatgaagtaa agccaaaaat cgaaaataac
     3901 taccctgaaa ttgcaccatt tgtaggtaaa ttaaagggtg atgatataga agaaggtaaa
     3961 gaattagtag caacacacat agctgaagga ttataccatg atggaaaatt tgttggttat
     4021 gtaaaaagag cacatgatgt agatgttaac ttaaatgccc atacaatgtt tgaaaatcta
     4081 gtagtaaaag catctggagt tttatctgct attcaaatgc taagacatag caaaatagac
     4141 cctgctgaaa tagattatgt tattgagtgt tcagaagaag cttgtggaga tataaaccaa
     4201 agaggtggag gaaactttgc taaatcaata gctgagatag caggacttca aaatgcaact
     4261 ggttctgaca caagaggatt ttgtgcagca ccaactcatg cattaattca agcagcagct
     4321 ttagtaaaag ctggtataca taaaaatgta atggtagtag caggtggagc aagtgctaaa
     4381 cttggaatga atgctaaaga ccatgttaaa aaaggtcttc ctgtacttga agatgttgtt
     4441 ggaggatttg cagtacttgt atctgaaaat gatggtgtaa atccagttat aagaactgat
     4501 ttaacaggta agcatactgt tggaactggt tcttcaccac aagcagttat gacagcactt
     4561 ataacttcag gtcttgatag agctaattta aaaataacag atgttgatgt gtattcagtt
     4621 gaaatgcaaa acccagatat aactaagcca gcaggagcag gagatgttcc agaagcaaac
     4681 tacaaaatga taggggcact tgctgttaaa cgtggagact tagaaaagaa agaattgaaa
     4741 gattttgttt ctaacaaagg attaccagga tgggcaccaa ctcaaggaca tataccatca
     4801 ggagctcctt atataggatt tttaatagat gatttaacta caggaaatag aaatagagct
     4861 atgatagttg gaaaaggtag tttattctta ggaagaatga ctaacttatt tgatggagta
     4921 tcttttatag ctgaaagaaa tacaggtgtt actgaagaga cttcaggaat atctaaggat
     4981 gaaattaaga agataatagc tgaatctatg aaaaaactag cattagatat gttagaagaa
     5041 taggggtgag gatatgtcaa aaaaagtaat cgcagatgta ttcttagaag tagctaatgc
     5101 aatagaaagc ggcgaatttg gcaaaaaagt aaaaattggt gttactacac ttggaagtga
     5161 acatggtgta gaaaacatgg ttaatggtgc acaattagca aaatctaatt tatttgatat
     5221 tgtgcttatt ggaccaaaag tggaaactga ccttgaagta gttgaagtta atgatgaaaa
     5281 agaaatgcat gcaaaaatgg aagaattatt agattcagga tatattgatg cttgtgttac
     5341 tatgcattat aattttccaa taggtgtatc tacagtaggt agagtaataa ctccagcaaa
     5401 aggaaaagaa atgatacttg caacaacaac aggaacaagt gctactaata gaatagaggc
     5461 aatggtaaga aatgccattt atggtattgc tacagctaaa tcaatgggta acaaatgccc
     5521 aaaagttgga atacttaatg ttgatggagc aagacaagtt gagaagtgct taaaagaatt
     5581 aaaagataat ggatatgaca tggagtttgc ggattcaatt agagcagatg gtggatgtgt
     5641 aatgagaggt aatgatttac ttgtaggtgc acctgatgtt atggttactg atacattatc
     5701 tggaaatata tttatgaaag tattttcttc atatactaca ggtggagatt atgaagctca
     5761 aggctttgga tacggacctg gagttggaga ggactatgat agaaaggtac ttatagtatc
     5821 aagagcatca ggttctccgg tagtagcaaa tgctttaaaa tacgcttatg atgttgtaaa
     5881 gggagatata agtaatgttg caagaaatga atttgcaaag gttaaaaaag caaaatttga
     5941 tgacattata tcctctctta ctaaaaaaga agttaaagct gaaaaagtag aagttaaaat
     6001 gccagataaa gaaattgtta ctcgtcaaat agctggtgtt gatataatgg atttagaaga
     6061 tgcagtatct gagttatgga aaaacggaat ctatgctgaa agtggtatgg gttgtacagg
     6121 tccaatagtt ttagtaaatg aagctaaagg agacttagca gttgaaactt tagttaaagc
     6181 tggatatact gcaaaataag tacatttttt atatgtaaat aagaacatat agttataatt
     6241 ttattgatta taattatatg ttctttttat tttatatctt gtcaatattt atatattaac
     6301 acaatccatt tatttgattc tctagctaat ttattatatt tattcagtta aaattaagct
     6361 ttctaactaa ataaaaacta atgaagtatt aaaatgaaat tgataaaata tgaaaaaatt
     6421 ttaggaataa agtatctgtt agataaaaat aagattaatt agtgatataa tataattaag
     6481 ataaaattta ttatttggag gcaaaatcat gagcagagta aaaaatgtag tagaattgtt
     6541 ggaaactaaa ggtgttgatg cactatatct aacaaagaaa actaatgtaa actatataag
     6601 tggttttcct gatgaagaag catatgcagt gatttgcaaa gatggaaact ttttagttac
     6661 tgatagtaga tatatggagc ttgcagaaaa ggtttgcaaa gattttgaaa ttataaattg
     6721 gcataatttt gatagaagtg tggcaaaggc agttaaaagt gtatgtgata aagtgggtat
     6781 aaagaaactt gggtttgaaa gaactaatat agtgtttgat aaatatgaag aacttaaaaa
     6841 tctgattgag aaggataatg gagaattaat acctacagaa aatattgtag agactttgag
     6901 atatgtaaaa gacaaagatg aaatcaaaaa tacaagaaaa gcttgtgaaa tagcggataa
     6961 ggcattagaa gaattgattc cacatataaa agctggagtt agcgaaattg aactggctac
     7021 aaagttagag tactttatga aaatgaacgg agcacaaaat atagggtttg aaactatact
     7081 gatatcagga gcaaagactt cattgcttca tggtaagcct agtgataaga ttatagaaaa
     7141 aggtgatttt gtacttatag actatggcgc tatgtataat ggctatatat ctgatacaac
     7201 tagaactttt atagttggag gagcttctga aaagcaatta gaaatatata atttagttaa
     7261 ggaagctcaa aatgtagggg ttgagaacat gaaagctggt gtacatgcga caattccaga
     7321 tgctgagata agaaaagttg tcaaaaaata tgaagactat tactatcaag gtataggaca
     7381 tggagtcggt agagatgttc atgaagaacc tttcattggt aattatggtg ataaaatcat
     7441 agaagaggga tgtataataa caatggaacc aggtatttat ttccctggat ggggtggagt
     7501 tagaatagaa gatactgttt tgattactaa aaatggtcca gaaagactta ctaaattccc
     7561 taaagatttg atgattttag acaaataatt taatttatat atattttttt acataaaata
     7621 taacataatc tcactattaa gctctattta catattatag taaagagggc ttaattttat
     7681 tgtgagaggt gatagtatat atgtataaaa aacataaatt tataaaaaat ataaaaaaag
     7741 cctggtataa aatcaaatta attgatagat gccttataat tgtaatggca atattaatgt
     7801 ttcaatctat atataattta tttgttaatg aagtaaattc acaagatact acaactattg
     7861 atgtagtagt aagaaccact tcagcagcta tatttgggta ttttttaagt gctaatttta
     7921 taaagagacc tttaaggaag tatacaaata ctgatgtatc ttcaaatctt tttatagata
     7981 ataatttatc cccaaaaggt caagagtcta atcaaaataa tataatgaat gtcaaaaata
     8041 ccattggatt tacttcagaa gctgataatt atgaaaagaa aatacctatt aataataatg
     8101 aaggttttga agaaggagaa actagtgaat tgcaaattat tatagctact gtaatttgta
     8161 tagtagcatt aacagtactt tttgttgtaa gaaattttac aactacaaca tcagcttctt
     8221 taggtactat ttcacagatg agagattttg tctcaggatg cgtgggtttt ttgctaggtt
     8281 gtccaagtaa acctgattaa aaaagtatta tcaataaaag gtattttaaa aagtatcagt
     8341 ataataatat ttagatttaa atgtatacaa ttataattct aaacattcta tattgtattt
     8401 ttatactgat actctaaact atactattta ttaaaatatt ttaacattgt atttaaacaa
     8461 aatattatat ctgtataata taaatttagt ttatatgaat agatgccata tactcaataa
     8521 aaagtttcat actatgagat atgtattgat ttcttctcca aagaacccca atattcgcag
     8581 taattgcacg ttctaatggt attgaacaga gattttctgt attttgaaat atttctttat
     8641 acaatattgt tgaagccaag tcattttcta gtaaataacg tatggtagaa agttgatttg
     8701 agtgtaaaat tatatttggc ttagcttttt caagtctttt tgtaataatc ttatagtgat
     8761 aagaaccttc agataagatt acaaaaggta cattttttaa catataattc gatataatgg
     8821 gatgttttgc caattcattt ttgggatttg taactaagta taattctgtt ttatagattg
     8881 tctttgaatc tatatcatca taaatatcac tgtcaattat accaatacaa aaatcaagtg
     8941 tagattcttc aatcatttta gctccaacaa atgttggaac ttcaaaaatt ttaagctcta
     9001 tatctggata aattacatta aatctaggaa gtatttttga aagcaaaaac gtacctaaaa
     9061 ctgtaggaat acctattttg agagaagctt ttctcttttt tcctaaatca agtgcgtcac
     9121 attgaaaatc ctcaaattgt tttataaaat gttttgactt gtctaaaaaa atttttcctt
     9181 caggcgttaa ctctatttta ttacctattc tgtaaaaaag ttcaaagcca agttcttttt
     9241 ctaattcttt aattgctaat gttatggatg gttgagaaat atgaatactt tccgcagcct
     9301 tagtcatgtt tttatattta cagacagttt gaaaatagta taaatagttt atattcattt
     9361 ggtattagtt cctttcattt acatattagt gacaagtata taattaacaa tagcataagt
     9421 tttgtatatt gttgtaaaaa aatagaatag ttttaaacta tcatagcata agtttttgat
     9481 attttcaatc tattaagaat agtgctatta ttaaattaac aaaagagaat agctggtatg
     9541 gtttagagta aatcaatcta tattacagtt atatcaacga atatcaaaaa attatctatt
     9601 agaaaataaa aattattttt acaattaaat tgttaattaa atcataaaga aaaaagtgat
     9661 attaacaaga taaaattttt ttaatcagaa atgttaattt aataacaaaa tagagtattg
     9721 gggagaaagg agaaatatat tttttaataa tatttgttaa tttaataacg aaaaatgaaa
     9781 gggagtgatt tagtgaagtt tgaattgatg gattttttaa tgaatccttt tgtccttatg
     9841 tttgctgcag taattacagg aattttattt ggaaaaatta aatttgggaa gtttaatttt
     9901 ggggtatcag gagcattatt tacaggatta tttattggat ggcttgcata tagcttgggg
     9961 aatctaataa tagcaaaagg ggaaacagca gcaggatata aggcagcaac tgtaatgatg
    10021 ggtaatggaa ttatttcttc tgacttcttt gatttctttt taataatatt tgttgcagca
    10081 gttggtctat tggcagcaaa agatatgaaa gctgtattaa agaagtatgg agcaagattt
    10141 gtaattttag gagttcttat aacttttata ggtggctcta tgacttatgc aatgacttta
    10201 ctatcaagtg ataaaggaag tagtgcttat gaagtttctg gcgtttacac aggagcatta
    10261 acaagttcgc ctggattagc tgctgcttta gaaacagctg gtaaacatgc agaagatgta
    10321 tcaaaagaat ttgaaaaagc ttcaataaaa gataaaaaag aaattttaaa agtagtcgac
    10381 ccagaaggca aattggatgt gaatacaact acatcactga ctcaagagca aatagataag
    10441 tatattgcat atgctgaagc tggtgttggt ataggtcatg cagtcgcata tccttttgga
    10501 gttttaatag taatcttagg tgtaaatttc cttccaaaat tatttaggat ggacctaaaa
    10561 gaagaaagaa gaaaatatga gaaggaaatg aaggaagcaa gagatagtgt ttctggtaaa
    10621 acgataccag aagttccatt taatataata acatttttct tgacatgttt agctggatac
    10681 ttagttggag gaatacatgt atttatggga ccactagggt acttcacact aggcgcaaca
    10741 ggaggttcac taattgtatc tctagttctt ggatatatag gtaagatagg ggtagtaaat
    10801 ttcagaatgg aagaaaaggt tttaaatatt ttaaaacaaa tagggctagt atttttctta
    10861 gcaatagttg gattaagata tggtggaaaa gtagttgatt caattatgac atctggtatg
    10921 cacctagcct tagtagcaat agcagtaggt gttacagcta tgatgatagg attcttagtt
    10981 ggcaaatacg tatttaaatt aaattggatt ttattatctg gagctgtctg tggtggtatg
    11041 acatcaacac caggtcttgg agctgctgtt gatgctcttg atagtgatga cccagcagca
    11101 ggttatggtg ctacgtatcc atttgcatta ttaactaaag ttatattggt tatagtttta
    11161 cacaaacttc caatgtaaat aagattttat atggggggat atttatgaat aatgaaggag
    11221 catttgaaga attaagtgga agattaagaa ataaagaatt gtgtgattta ataatgaatg
    11281 cagatgaagc tgctaaaatt atagaggatg gcatggttat tggagttagt ggatttacac
    11341 cttcaggcta tccaaaagca gtaccattag cagtttcaga aagagctaaa agtggagaaa
    11401 agataaaatt aaccgtatat tcaggagcat ctttaggtcc agaagttgat ggagcatggt
    11461 cagaagcagg tataattgaa agaagacttc cttatcaaac taattcaata cttagaaata
    11521 atattaataa aggtgttgtt gattatatag atatgcacct aagtcattct actcaatttt
    11581 taaattatgg aactatacct aaagtagatg tagctatagt agaagcactt gcaataactg
    11641 aagaaggaaa tattatacca actagtggga taggtaattc tccatctttt ataaaaagtg
    11701 cagataaagt aatagtagag ataaatcttg caaaaccaat ggaaatggaa ggtatggcag
    11761 atatatatat tacggaaaat ccaccaaata gaaaaccaat agaaataaat catcctaagg
    11821 atagaattgg aacaacatat ataccttgtg gtttggataa aattgcagga gtagtaatta
    11881 ccaatatgca agataagacc cgtccattgg gagttgtaga tgaagcttcg aaaaaaatat
    11941 caaataatat tattgctttt ttaagagaag aagtaaaggc aggcagatta tcaaagaatt
    12001 tacttcctct tcaatctggt gtaggaagtg tagcaaatgc agtattatat ggattatgtg
    12061 aatctgaatt tgaaaattta acttgttata cagaggttgt acaggactct atgttggatt
    12121 taataagaat gggtaaagtt acaatggcat ctactacatc tgtaagtcca tctccagaag
    12181 gattaataaa atttgaaaaa gatattgatt tttttaagga taagattata ttaagacctc
    12241 aagaaattag taacaatcca gaaatagcca gaagaattgg tgtaattgca atgaacactg
    12301 caattgaagt tgatatttat ggaaatgtaa attctactca cataatgggg tcaaagatga
    12361 tgaatggaat tggtggttct ggtgattttg ctagaaatgg agcaataact atatttagta
    12421 ctgagtcaat tgcaaaaaat ggtgatatat catcaatagt acctatggta tctcacgtag
    12481 accacactga acatgatgta atggtaattg taacagaaca aggatatgct gatttaagag
    12541 gtcttgcacc tagagaaaga gctataaaaa taatagaaaa ttgtgcccat ccagattata
    12601 aagaacaact tagggattat ttaaatagag cttgtaaaag tggagcaaaa caaacacctc
    12661 acatattgga cgaagcttta tcatggcata gtaaatttat gagtacaggt acaatgaaaa
    12721 aggctgaaac ttttaaaagt gcattataaa atatttaata aattggaggt agattttaaa
    12781 tggaaaaagc agtggaaaac tttgaagatt taagtaagga atacattaat ggttatattg
    12841 agagagcaag aaaagctcaa agagagtttg aatgttatac tcaagaacaa gtagataaaa
    12901 tagtaaaaat agttggaaaa gtagtgtatt ataatgctga atatttagct aaacttgctg
    12961 tagaagagac tggaatggga gtatatgaag ataaggtagc taaaaataaa agcaaagcaa
    13021 aagttatata taacaattta aaagataaaa aatcagttgg tataattgat atagatagag
    13081 aaactggaat aacaaaggta gctaaaccag ttggagtagt agcggcaata actccttgta
    13141 caaatccaat cgttacacct atgagcaatg caatgtttgc acttaaggga agaaatgcaa
    13201 ttattatcac acctcatcat aaagcaattg gatgtagtac aaaaactgta gagatgatta
    13261 atgaagaatt agaaaaaata ggagctccag aaaatttaat acaaatactt gaccaacaat
    13321 caagagaaaa tacaagaaat ttaatttcat cagcagatgt agtaattgcc actggaggaa
    13381 tgggaatggt aaaagctgcg tactcaagtg gaaaaccagc acttggtgta ggggcaggaa
    13441 atgttcaatg tatcatagat agagatgtag acattaaaga agcagtacct aaaatcatag
    13501 caggaagaat ttttgataat ggtatcattt gttcaggtga acagtcagta atagtagcag
    13561 aagaaatgtt tgataaaata atggatgaat ttaaaaataa taaaggtttt atagttagag
    13621 ataaagtaca aaaagaagct ttcagaaatg ctatgtttgt aaataaatct atgaataaag
    13681 atgcagtagg tcaatcagtt catacaattg caaaaattgc aggtgttgag ataccagaag
    13741 atacaaaaat aatagtaata gaagctgatg gaccaggaga agaggatatt attgcaaaag
    13801 aaaaaatgtg tccagttata tctgcataca agtataaaag ttttgaagaa ggtgtagcta
    13861 ttgcaaaagc aaatttaaat gtagaaggaa aaggacatag tgtatctata cattcaaata
    13921 cagttaaaaa tatagaatat gcaggagaaa atatagaagt atcaagattt gttattaatc
    13981 aatgttgtgc tactagtgct ggaggaagtt tctttaatgg tcttgcacct actaatactc
    14041 taggttgtgg ttcttgggga aataacagca tctctgaaaa tcttgattat aagcatctaa
    14101 ttaatatttc tagaattgct tattatatgc cagaaaatga agtccctaca gatgaagaac
    14161 tttggggata aaatttagac aaaaaatatt cattctaaat taaagtagga atatatttta
    14221 atttagaatg aatttaagac ataaattaaa tttaaattag tttattggac taaatttaaa
    14281 atattaagag acatatagaa gaaggaagta ttataattta aaataaataa aaaaatctat
    14341 aaaagagaag gagtggtttt attatggcat taatgacagg agctcaatat attgaaagtt
    14401 tgagaaaatt aaatactaag gtttatatgt ttggtgaaga ggttaagaat tgggttgacc
    14461 atcctatgat tagaccatct ataaattgtg tagcagcaac ttatgactta gctcatgacc
    14521 cagaatatgc agatttaatg acagttactt caaatatcac aggtgaaaaa ataaatagat
    14581 ttggtcatct tcatcaaagt gttgatgatt tgataaaaaa agtaaaaatg caaagactat
    14641 gtggtcaaaa aacagcttct tgtttccaaa gatgtgttgg tatggatgcg tttaatgcag
    14701 tgtattcaac aacatttgag tgtgataaag cccatggaac taattatcat gataattttg
    14761 taaaatactt aacttatata caagaaaacg atttagtagt tgatggagct atgactgacc
    14821 ctaagggaga tagaagtcta tcacctagtg ctcaacctga cccagatatg ttccttcata
    14881 tagtagaaag aagagaagat gggataattg taagaggagc aaaggcacat caaactggtt
    14941 caataaattc acatgagcac cttataatgc ctactataag tatgactgaa gcagataaag
    15001 actacgctgt atcatttgca gttccttcag atgctgaagg agtgtttatg atttatggaa
    15061 gacaatcttg tgacactaga aaattagaag aaggtgcaga tgttgattta ggaaataagg
    15121 agtttggagg tcaagaagct ttagtagtat ttgataatgt atttgttcca aatgatagaa
    15181 ttttcttatg tggagaatgg gatttctctg gtatgttagt agagagattt gcaggatacc
    15241 atagacaaag ttatggtgga tgtaaagtag gagtaggtga tgtaataata ggagcagcag
    15301 ctttagcagc tgattataat ggagcaaaca aagcttcaca tataaaagat aaactaatag
    15361 aaatgactca ccttaatgaa agcttatatt gctgtggtat agcatgttca tcagaaggtc
    15421 acaaaacaga agctggaaac tatcaaatag atttacttct tgcaaatgtg tgtaaacaaa
    15481 atgtaactag atttccttat gaaatagtaa gacttgcaga agatatagct ggagggctta
    15541 tggttactat gccttcagaa aaagacttta aatcagacct taaagttgga acaagtggaa
    15601 tgactattgg cgaagtatgt aacaagtatt ttaaagcttc ttcagtggct tcaacagaag
    15661 aaagaatgag aatattaaga ttcttagaaa atatatgcct tggttcatct gctgttggtt
    15721 atagaactga gtcaatgcat ggggcaggtt caccacaagc tcaaagaata atgatttcaa
    15781 gacaaggaaa cataaatcaa aagaaagaat tagctaagaa aattgcggga ataaaaaaag
    15841 aggaagcttt aaatttataa aaagttgata tataaaggga aattatatat taatgtgtgt
    15901 taattaatga aaaaatatgt agtataagag atagttataa aattatggtg gtttttagct
    15961 aagtaaagaa atttttaata aggagaattt acacatgacc tgtggagtta gattttgtgg
    16021 gggctgtaac cctagatacg aaagagggaa ggctcttaca ataatcaaag agcacttcaa
    16081 tggtagggta gattttagta tagcacaaga gaacgtcgag tatgatttgt tacttataat
    16141 tggaggatgc gcaactctat gcgcatccta tcaacaatat aaagtgaaaa atgaatgtgt
    16201 taagatgtgg gattttagcc atatagaaag agttataaat gtgattgaat cagcaatttg
    16261 tgaaacttaa aaaagctaaa tggggggatt tcattgagtt ggcaagaatt gtatcaaagt
    16321 aaattatgta gtgctacaga agctgtaaaa cagattaaaa acggtgatac tgtagtattt
    16381 gcacattgtg taggtgaacc tcctgcacta gtagaagcta tgattgaaaa tgctgaacaa
    16441 tataaagatg ttgagattaa acatatggta agcttgggta gtggtggata tactgctaag
    16501 ggaatggaag ctcattttag agtaaatcca atgtttgtaa gtggaaatgt aagaaaggca
    16561 attgaaaatg gagatggtga ttttacacct gcattcttcc atgaagtacc aaagttgctt
    16621 agagaaaaaa gattaaaatg tgatgttgtt ttagcacagg taactccacc agatgaacat
    16681 gggtattgta gcttaggaac atcagttgat tatacttatg aagccattaa aactgctaga
    16741 acagtaattg ttcaagtgaa tgaccagttt cctagaacct atggtgaggt agtccatgta
    16801 tcagagtttg actatatagt ggaaaaatca caacctttat ttgaactaca acctgcaaag
    16861 attggagaag tagaagaagc aattggtaaa aattgtgctt cattaattga agatggctct
    16921 actttacagc tagggattgg tgggatacca gatgcagtta tgttattttt aactgataaa
    16981 aaggatttag ggattcattc agaaatgatt tcagatggga ctttggccct ttatgaaaaa
    17041 ggtgttataa atggtaaata taaaaatttt gataaagaaa aaatgacagt taccttctta
    17101 atgggtacta aaaaattata tgactttgca aataataacc ctgcagtaga ggtaaaacca
    17161 gtagactatg tgaatcatcc agcaattata atgaaacaac ataagatggt ttctataaat
    17221 tctgctatac aagttgattt aatgggacag gtagttgcag aggctatggg acttagacaa
    17281 tttagtggtg ttggaggtca agttgacttt atacgtggag tgtctatggg tgaagatgga
    17341 aaggcaatta tagctatgcc ttcaataact acaaaaaaag atggtacagt aatttcaaaa
    17401 atcgtatcta ttgtggatga aggtgcacct ataacgacat caagaaatga tgttgactat
    17461 attgtaacag aatacggtat agcagaatta aaaggaaaat ctcttagaga aagagcaaga
    17521 aatttaataa atatagctca tccaagtgta agagaacatc tagcagtaga atttgaaaag
    17581 agatttaagg agaaatatta ataatagagg ggtgttaata atggaagcac ttagagtagt
    17641 tccaaagata ttttactttg atacatttaa agaatttaat gaagaattta aaataggaaa
    17701 aaatgattta gtaattacta atgagtttat atatgagcca tatatgaaac ctcttggaat
    17761 agatacaaat ttaatttttc aagaaaagtt tggaacaggt gaaccatctg atgaaatgat
    17821 agactccatg acaaaggaaa tgaagaaata caattttgat agaattattg catttggagg
    17881 aggaacaata gttgatatat gtaagatatt agcacttgat gttccagaaa agtctattga
    17941 tttatttgaa ggagaagtat cacctaaaaa agtaaaagag ttagtagtag ttcctacaac
    18001 atgtggtaca ggtagtgaag taacaaatgt agctattgca gagttaaaat ctaaacatac
    18061 taaaaagggg cttgcagtag aagaaacata tgcagactat gcagtgttaa ttccagagac
    18121 agtaaaaggg ttaccttaca aattctttgt tacaagttcg gtagatgctc ttatacatgc
    18181 tattgagtct tatctttctc caaaagcaag tccatttaca gagatgtatt ctctacaagc
    18241 aataaaaatg attatggatg ggtataagaa gatagttgat aaaggagaag aagagagatt
    18301 taatcatctt agagactttg tacttgcatc aaattatgca ggaattgctt ttggaaatgc
    18361 tggttgtgca gcagttcatg cactatctta ttcaataggt ggagctttcc atgttgcaca
    18421 tggtgaagca aattatcagt tctttactga agtatttaag atgtattcta gaaaaaaacc
    18481 taatggaaaa attataaaat gtacaaaaat tttagcagat gcccttgaat gtgaccctaa
    18541 ctgtgatgtt tatggagaat tagaaaagtt tttaaataaa ttaatagcta aaaaagcttt
    18601 gagagaatat ggaatggtgg aaagtcagat agatgaattt acagatagta caatagcaaa
    18661 tcaacaacga ctacttgcta ataattatgt agagcttagt agagaggaaa taagagagat
    18721 atttgcaaat ctttattaat acaatttttt atttatatgt atagtcaaca taaacaatgc
    18781 taattatatt tatgtacaaa taaaaataag ggaacttaaa ttttaattaa agttaagttc
    18841 tcttattttt atatggagtt aaatataagt atatttttaa tttaaaatat gcttatattt
    18901 aagtctatta ctttatttga aacaatttgt agaatttcaa attccttgac agtaataagt
    18961 aagtaattgt ataatcaaac tgcaatatat taataatatt agtgaaaatg aaaggattat
    19021 aaaatatgta taaatcagaa aatttttcaa aaaaatctgt attaataaat tcatttttaa
    19081 gtaaattttt aaaaataatt ggatggggtt tggtaagctt atttacatta tcagccttag
    19141 tactttcaac tatttccatt ataattggat gggctgtaat acttcctatt caagtatgtg
    19201 cagtattttt acttatagga ggagtttgta tttttaaagg taagagatta ggagaccaaa
    19261 tttctcgtta taaaaaatat tgtacaatta taaacaatag aaatattatt ccagtagaac
    19321 ttattgcaga aaacacatct aaatcattaa attttataat aaaagatgta caaaagatga
    19381 tagacaaaaa ctactttata aatacttata ttgataaaag aaataatcag attgtgttaa
    19441 caaatgaaga ttttgttcca ccaagatatg aagatgtagc atatgaagtc aaagaagaag
    19501 aaaagactga tgaatccaat gaagttgata caattataaa taaaggaatg aattatctaa
    19561 cacaaataaa agaagctaat aaaaatatta aatcagaatc aatgggcgat aaaattgtac
    19621 aagtagaaga tgttacaagt aaaatatttg atgttgtaaa acatgaccca tcaaaactta
    19681 ctcagataca aaaatttatg gattattatc ttccaaccac tttaaaacta ttaaattcat
    19741 atcatacatt ggaagaacaa ggaattgata gagaaaatat aacaacaact atggaaagta
    19801 ttgaaaatac aatgaatacg attgtagtag cttttgaaaa tcaattggac tatctgtttg
    19861 aagatgaagc cattgatatt tctacagata ttactgtttt agagaatatg ttagtccaag
    19921 aaggattaac gagtggtaaa ttttaacttc aatagaaaaa atattctgtt atttttaaac
    19981 aattaattag tcataaagta taaaatgtgt attatataag ttaatcagta ctttatgaag
    20041 gaagaggaga gaaagataca atgaataatt tagatgatat accagttatg ccaactttga
    20101 cacttgaccc atttggagaa gaatcaaaca ctaatgatat agataattct gacttattaa
    20161 tgaaaaagga tgaaaaagac ccagaagaag aaaaactgtc tgaatctgaa cgcaagatgg
    20221 taaaggaatt tgcggaaaaa atagatatta caaatacaaa tatgattttg caatatggag
    20281 ctggagctca aaaaaaggta gcaagttttt ctgaaacagc tttaaagagt gtaaaaacaa
    20341 gagatttagg agaagttgga gatatgctta caaatctagt tactgattta aaagcatttt
    20401 cagcagatga aaaagaacaa agtggttttt taggaatttt taaaaaggca aataataaaa
    20461 tttcaaattt aaaaactaag tatgatagtg cagaggtaaa tgtggataaa gtaagcaaag
    20521 agcttcaaaa gcatcaggtt aaaatattga aagatatagc aatgcttgat aagatgtatg
    20581 aattgaactt agcttactca aaagagctta ctatgtatat tatagcagga aaacaaaaat
    20641 taaaggatat gaaagaatat gaaatgccaa agttgagaga aaaagctcgt ttatctggtt
    20701 caacagaaga tgctcaatct gtaaatgata tggtttcact ttgtgacaga tttgaaaaga
    20761 agattcatga cttagaatta actagaatgg tatcgcttca aatggctcct cagataagat
    20821 tggttcaaaa tagtaataat ctgatggcag aaaagattca atcaactata gtaaatacca
    20881 ttccattatg gaaaaatcaa atagtgcttg ctcttggaat ttctcactta aatcaagcta
    20941 tgaaagcaca gcgtgaagtt agtaatatga caaatgagct tttattaaag aatgcagaaa
    21001 ctttgaagat gggaacaata gaaacagcaa aagaatcaga aagaggaatt gtagatatag
    21061 acacaattaa gaaaactaat caatctctaa tatctactat tgatgaggta gttaaaatac
    21121 aacatgatgg tagaataaag agacaagaag cagaagttga attatcaaaa atagaaaatg
    21181 aacttaaaag taagcttctt gaatttacag ttaaataaaa attatactaa tataaaaaga
    21241 gactgtctca aaatagagat aagtctcttt ttcattgttt aaatgattaa agtatattta
    21301 tattataaat ttctattatc ttttaacata acatcatgag ctccaccttc aactatacta
    21361 gtagaagata caagtgtaag tttagcattt ttttgaagtt caggaatgct aagtgctcca
    21421 cagttacaca tagtagaacg aactttactt aaagaaagag ttacattgtc tttcaaagaa
    21481 ccagcataag gcacataaga gtcaacacct tcttcaaaag aaagtttctt atctccacca
    21541 aggtcatatc tttgccagtt tctagcacga gcagaacctt ctccccaata ctctttcata
    21601 taactaccat ttatattaac tttgttagtt ggactctcat caaaacgaga gaagtatcta
    21661 ccaagcatta taaaatctgc acccatagct aaagctaaag ttatatgatg gtcataaacg
    21721 ataccaccat cagagcaaat tggaatataa atacctgttt tttcaaagta ttcgtctcta
    21781 gcttgagcaa cttcaattat agaagtagct tgtccacgtc ctataccttt ttgttcacga
    21841 gttatacata tagaaccacc accaatacca actttaacga agtcagcacc agcttcagct
    21901 aggtaaagga atccttcgcg gtcaactaca tttccagctc ctaccttaac tgtatcacca
    21961 tatttttcac gaatgaaatc aagtgttatt ttttgccact cagaaaaacc ttctgaagaa
    22021 tctatacata aaacatcagc accagcttca actaaagcag gaacacgttc agcaaagtct
    22081 ctagtattaa taccagctcc aactacatat ctcttagaag aatctaaaag ttcaagtgga
    22141 ttttctttat gagaagagta atctttacgg aataccatat acataagatt accattatca
    22201 tcaagtacag gaagagaatt aagtttgtgg tcccatataa tattgtttgc ttcctttaaa
    22261 gtaacatctt tgttagcata aactatagat gacataggag tcataaattc actaacttta
    22321 gtatctaaat ccatacgact tatacgataa tcacgacttg caacaatacc caataattta
    22381 ccagttgaag ttccatcttc agtaacagct actgtagaat gtccagtttt ttcttttaag
    22441 tcaagtatgt cttttaatgt gttatcaggt ttaatatttg aatcacttac aacaaaacca
    22501 gctttgtggg atttaacacg tgctaccata gcagcttcat tttcaatagt ttgagaaccg
    22561 tatataaatg aaataccacc ttctttagca agtgcaatag ccattttgtc atcagaaaca
    22621 gattgcataa tagcagaagt caaaggtata ttcatataaa tatctgcttc ctcgcccttt
    22681 ttaaatttag taacaggtgt ttttaaactt acattagcag gtatacaatc agtagaagag
    22741 aaacctggta caagaaggta ttcactgaaa gtatgtgatg gtgtatcaaa ataaaacgcc
    22801 attttttaat cactccttat atttttatac tttttataat tgatagtatt aattataact
    22861 tattctttaa ttttattaaa gtattttgtc aaatattgag atttttgaaa aatttaaact
    22921 attttaatta aatttgttta atatatattg ccttataggg taaagttgta tattaaataa
    22981 tgtttagtgt ttaatagtag ctaatttaaa attaatcttt agtgattcat ataattacat
    23041 ttgtatagtt tacattgata atattactag agagtgcata aacctcaaaa atacagataa
    23101 attaaatcta tattagtata tccaaaagta catttaataa atttatttaa ctgaattatt
    23161 attttcattt ctattaaatt taaatatttt ataaaaatag tattatttac aaaatcatat
    23221 actccaaaat actacataaa taaaattaaa caaaaactaa aatgacaaat gtcattatct
    23281 aaagtgacat agaaacggtt tcttaaagtt gttactataa aatataaact ctataataaa
    23341 agtataacaa aagggaatca tatcgatata gaatataatt caagaggagg taaaattatg
    23401 agtagttcta atcaatcgac aataaagaca tctatacagc gagtagggaa atttttaagt
    23461 ggtatggtaa tgccaaatat aggagctttt atagcatggg gtcttataac agcattattt
    23521 ataccaacag gatggatgcc taatgaaaaa ttgagtacta taggtgaccc aatgataaaa
    23581 taccttttac cactattgat agcttatact ggtggtaaag caatagcagg acaaagaggt
    23641 ggcgtaatag gtgcagcagc agctatggga gtaattgttg gtgcagatat accaatgttt
    23701 attggagcaa tgataatggg accatttgca ggatgggtaa ttaagaagtt tgataaattt
    23761 gtagatggaa agataccaac aggatttgaa atgttagtaa ataacttttc aataggtata
    23821 ataggtatgc ttcttgcaat actaggattt tatgcaattg gaccagcaat agtagcaggg
    23881 acagctctta tagaatctgg agttcaattt atagttagca aaagtttatt gcctctagtt
    23941 tcagtattta tagaacctgg aaaagtatta ttcttaaaca atgcaataaa tcatggtata
    24001 ttagggccta taggtatagc tgaggcaaaa gaagcaggta aatctataat gttcttacta
    24061 gaaagtaacc ctggaccagg tttaggagtt ctattagcat attggatgtt tagtagagga
    24121 agtgtaaaac aatcagcacc aggagctgta ataatacatt tctttggtgg tatccatgaa
    24181 atttacttcc catatatttt aatgaatcca gttttaatat tggctacaat ggcaggtgga
    24241 gcagcaggaa tattgacttt ttcaatatta ggagctgggc ttgtagcagc accatcacca
    24301 ggaagtatat ttgcacttat ggcactagct ccaaagggag gattacttcc agtattagca
    24361 ggagttgcag tggcaacagt tgtatcattc ttagtggcag caccatttgt taagagagct
    24421 tcagcaaatc aaagtgaaga agacagtact tctttagaag aagctaaagc aaaaatgagt
    24481 gatatgaaat ctgcaagtaa gaactctgag aaaaatattg aggaaaaaca gcttgaagta
    24541 aatgagatta agaagattgt atttgcatgt gatgcaggaa tgggttcgag tgcaatggga
    24601 gcttccagat ttaaaaatag aataaaaaac ttagacttaa atatagaaat tacaaattca
    24661 tctgttgata atcttccaga tgatacacaa atagtagtaa ctcacaatac attagttgaa
    24721 agagttgcta agaataattc aagtgttgag atagtttcaa taaacaattt tttaaatgac
    24781 ccaaatttag atacattgtt taaaagatta gaatctaaat aaaaataaaa agtaccacta
    24841 attaggagtg gataatttga aaaaaaagaa gattacatct aggcaaaaaa agataatact
    24901 aatgatagta gcaaattcaa agaaaaacat tccaataact atttctgaga ttgcagggac
    24961 tttagagtta agttcaagaa ctgtacttag agatatgtct ggaattgaaa aatggtttga
    25021 tgaaaatgat tttaactttg taaaaaaacc aggagtggga ttgattttag aggaaaatat
    25081 agaaaatcaa aactttataa ttgagttgtt agaagaagaa aaaatagaaa aagaatacag
    25141 caaggaagaa agaaatttaa ttatattatc aaagctacta gtttctaacg agccagtaaa
    25201 atcctattat tttgcaaaaa tattaaaggt atctgaatgg gtactgaata atgattttgc
    25261 cttagcaagt aaatggttgg aaagatttga tattgagctt gttagaaaac cagggctcgg
    25321 tgtctactta aaaggtcaag aaaaaaactt tagagaggca tatgtaaatc ttatatacga
    25381 ttcattcaat gaaaaagaaa tactagatat ggtcagaaat ataagtgaaa atatacaaac
    25441 agataaagca atagaaattt taagtgaaaa tagactttta aatttaatgg acaggtgcat
    25501 aataagaaaa gttgaagcta cattaacaaa gaagctttca gatttagatg taaatttagc
    25561 tgatagtgca tatatagggc ttgtagttca tatttcatta gccttacaga ggataaaaaa
    25621 tggtgaaaat ataactatgg ataaagagtt tctaaaagaa ttatctataa cagaagagtt
    25681 taaattagct aaagaaatcg taaaaggtat ggaaacagat tttaacatgg atataccagt
    25741 agatgaggtt ggatatataa ctatgcatat aagaggtgca aagcaaaggt catcttcaaa
    25801 ccataaggct ttaaacttag atgatataga aataatggaa attacaaata agatgataga
    25861 tttggcggaa gatgaattta aaataagttt aaaaaatgac gagaggttat ttaaagacct
    25921 agcaaatcac ttagggcctt ctataaatag gttgaatatg ggattagaaa taagaaatcc
    25981 tcttttagat gaaataaaaa gcaagtattc atatgcctat gatgtagttg aaaagatttc
    26041 tagaattata aaagataaac taaatataaa ttcaatacct gaatcagaaa taggatatat
    26101 tgcaatgcac tttgcatcag ccatagaaaa aaatcttatg atgaatacta atataaatat
    26161 tgttgtggca tgtcccactg gaataggtac ttcaagattc ttatcaacta agattgaaaa
    26221 taaatttcct aatttaaata tacttgaaac catatcagct ataaacatag atgaagagta
    26281 tttaaaggaa aaagatgttg atttaatagt gtctacagtt gagttaaata caagtttaaa
    26341 ttatatatgt gtaggtccat ttatgagttt agatgatgaa cagataatca aggaaaaaat
    26401 aaaatctata gctcaaaata aattaatcaa tttaaatgcg aaaaatgata ctaagagtaa
    26461 gaataaagta tatgaacaga taacagaaag tatgaatata ggaaaagata ttcttcaatt
    26521 tttagaggaa attagatttg aaaaatttga aagtaaagat ttaagtgagc ttattgaaga
    26581 ctcatcaaaa atatttgcaa aatctagtga agatataatc tctataaaag aatcgttaaa
    26641 agaaagatta aaaatttcaa ttccatatat agaagaatca aaaattctat tgttacattg
    26701 tatgagtgaa agaatagata ttatgaagtt atcaataata aaactagaga atagaatcgt
    26761 gctagattca aatgaagaaa tcgacaatgt agtttttatg ttacttccta aaaattcacc
    26821 aagttatcaa agacaaataa tgagtgaaat aagtggttca ttaatagaca atataatttt
    26881 tactaataag ataaataaat tctctataga agagatggca ttagaggtaa aagatatagt
    26941 ttttaatttt tatacaaaca gattaaaagc atttatagat aaataaaatt aatattaaaa
    27001 aggaaggttg atactatgag taaagtattg aatgagaata atatattttt agggttagat
    27061 agtgtttcaa aggaagaagc tataacttta gcaggaagaa aattagttga aaatggatat
    27121 gtaaaagaag aatatatacc agcaatgtta gaaagagaaa aagtaatgac tacatatatg
    27181 ggaatgggag ttgctattcc acatggtgta aatgaagcaa aaaaagaaat attatcttct
    27241 ggaatagtta tattacaatt ccctaatggt atagattttg atggagaaaa agcatattta
    27301 ttaataggaa tagctggtgt tggagatgaa catttagaaa tactatctaa tatagcagta
    27361 gttttagatg atgacttaac agaaagacta aaaaattcaa atgacaaaca agcttttatg
    27421 gaagcttttg ccaactaatc ttaggaggga tttaaaatga aaaaggcaat tcagtttgga
    27481 gcaggaaata ttggaagagg ttttattgga ggcttgttag ttaagtcggg ttatcatgta
    27541 gtatttgctg atgtaaatga ggagatatta aactcaataa acaaagataa aaagtatact
    27601 atacacataa gagatgtaga atgtatagat gaagtaattg ataatataag tgcggtttca
    27661 tctataaaag aagaaataat agatgagatt gtacaggctg aaataataac tacagcagtt
    27721 ggacctttag ttttgactaa aatagcctct acaattgcaa aaggtataaa ggctagaaaa
    27781 gaaaagggat taacaagtaa tctaaatata atagcatgtg aaaatgcaat atacgcaagt
    27841 tcttctttaa aagaagaggt acttaagtat ttaaataagg aagaagtaga atatttagag
    27901 atgtatgttg gttttccaaa ctgttcagtt gatagaatag ttcctcctgg taaaaacgaa
    27961 aatcctcttg atgtaacagt tgaaaatttt tatgagtgga atgttgaaaa acaaggattt
    28021 aaaggggaaa taccaactat agttggtatg aatttagctg ataatcttat ggcttatata
    28081 gaacgtaaat tgtttacact gaatacaggt catgctataa cagcttatat aggatattta
    28141 aaaggatata agactataga agagagtata aaagataaat ttatctgtga tatagtcaaa
    28201 agtgcaatgg tagaaagtgg agaaggattg attaaaaaat acaattttga ctcagaagtg
    28261 cactataaat atattgataa aattttaaat agatttaaaa atccatatct taatgatgat
    28321 gtattaagag tcggaaggga accattaaga aaactaagtg ataaagatag attgattaaa
    28381 cctttaatga cagcaaaatc atatggatta tcagttgata atttgattct tggaataggt
    28441 gcagcgcttc attataataa tagtgaagat actcaaagtg tagaattaca agagttgata
    28501 aagtctattg gagtaaaaaa agccgttgct aaaatagcta atataagcaa tgacgaagaa
    28561 cttttaaaca atatcgaaaa aagttatata tttatgaaaa atttataaat ttacttgaaa
    28621 aaaagtagtt ctgataaagt aactataata tattattcta cacagtttga attatttgtt
    28681 gaataataga gaattataat atataattaa aaggaataaa ttaatatatt tacctgtata
    28741 tgtttgataa tatggttcaa atgtttctac taagctacca ttaatagctt gactacagga
    28801 gtgaagaaga tatagtttat acttatttta aattatatat taagttaatt actttgaatt
    28861 ttaagctcct gtctatgata tagacaggag cttttttagt attttaatat aaaatactta
    28921 caagaataga tagcaaaatt agaacaaata taattttttt attaatataa ttttagaaag
    28981 ggctaggtgg aagttcaatg aaggctttac aagaaaagat attgagagaa ggcagtgttt
    29041 caggaaatga tatactaaaa gttgatagtt ttttaaacca tcaaattgat gtagcttttt
    29101 taaatgaaat aggaagagaa tttaaagaga gatttaaagg agaaaaggtg gataagatat
    29161 ttacaataga ggcttcagga atagctatag catcaattgt ttcacagtat tttgataatg
    29221 cacctgtagt atttgcaaaa aaatcagaat ctaaaaattt ggataaagat gtatatgaaa
    29281 ctaatgtata ttcatttact aaggcaagag agtattcagt taaagtttct aaaaagtaca
    29341 taaacaaagg cgaaaatata ttgatagtgg atgatttttt ggcaaatgga agagctgctc
    29401 ttggacttaa agatttaatt gagcaagcag aagctaactt agttggagta ggaattgtca
    29461 ttgaaaaagg atttcaagct ggaggagcac ttttaaaggc taatgatgta agattagagt
    29521 ctttggcagt agttgaatct atagataatg gaactgtaaa atttaggtaa tttatgatat
    29581 tatatacttg tgtttagcac atattttaac tatgtaatta tggaggattt tttatgagat
    29641 tttttataga cacagccaat attgaagaaa taaaagaagc aaatgattta ggtgtaatct
    29701 gtggtgtaac tactaatccg tctctaatag ctaaagaagg tagagatttt atagaggtag
    29761 ttaaagaaat aagtgaaata gtagatggac caataagtgc agaagtaata agtttagaac
    29821 ataaaggtat gatagaggaa gcagaaaaat tatctaagat acataaaaat atagttataa
    29881 agatacctat gacagcagaa ggtctaaaag cggtgaaagt actatcaagt aaaggtataa
    29941 agactaatgt aacgcttata ttctctgctg gacaagctct tttagctgct agagctggag
    30001 ctacatatgt aagtcctttt gttggtagat tggatgatat atcacaaaat ggtcttgatt
    30061 taattgaaga aatcgttgat atatttagtg taaatagtat tgaggctcaa ataatagttg
    30121 ctagtgttag aaatccaata catgttttac aagctgctag aatgggagca gatatagcaa
    30181 cagttcctct taaagttata aaccaaatga ttaagcatcc tttgacagac aaaggaatag
    30241 atagctttat gaaggattgg gaaggtgctt ctttaaaatt ataattataa actacgcttt
    30301 ataagtatat taaagaggtt attatggaaa ttctaagtaa taaggctagg ctattttgcc
    30361 tagtcttatt ttggtgtata atatattgaa acatattatt gaaatagatt aattttttaa
    30421 gtgaggaggc gaaaaatgga taaaatatta agagagatac aaagagaaga taaaaaaaat
    30481 ccatatactg accaagaact tgctgaaatg ttaaatgtag ctaggtctga agtgatatca
    30541 gttaggaaaa aaaataatat tttagattcg agagaaagaa gaaaaaaaat attaataaaa
    30601 gatatatcaa aaattgtaag tgaaaatcct caaatgtctg aaagaaaaat aactgaaata
    30661 ttaatagaaa atggatataa tatatcgaga agtgcagttt caaaattatt aaaagaggaa
    30721 aatttaaatc agtcaaagat tgggattaag gataaagacc cagcatcatc aggaaagtat
    30781 gtgaaggtga gtaaagttgt agaagaagat ggatttgaag aattgatagg tataaaggga
    30841 agtttgaaag aaaaggtaaa tctagctaag tcagcaataa tgtatcctcc taatggcctt
    30901 catactataa tatatggaga aacaggagta ggtaaaagtg aattagctac atgtatgtat
    30961 aaatatgcta taaaaaacaa cattaaagaa gagaacagcc catttatagt gtttaactgt
    31021 gcagactatg cagaaaatcc taatctattg atagctcaac tctttggggt agtaaaaggt
    31081 gcatatacag gagcagatgc aaatagagaa ggtcttgtag aacaagctaa taatggaata
    31141 ttgtttttag atgaaattca tagattgcct gctgctgggc aagaaatctt attttcatta
    31201 atagatagag gagaatttag aaggttagga gaaagtagtt ctaatagaaa ggcaaatgtt
    31261 ctcataataa gtgctacaac agaaaatcca gattctaatt tacttcaaac atttagaaga
    31321 cgtataccaa tggtaataaa tttacctaat atgagtgaaa gaccaaaatc tgaaagatat
    31381 gaaataataa taaagttttt tgaaagagaa gcaaagcgag taaacaagaa ttttataata
    31441 actaaagaag ttatgtgtgc acttatgaat tataagtgta ctggaaatat aggtcagtta
    31501 aagagtgata tacaagtaac ttgtgctaga gcttttagta aatcgatatt ttctagcgat
    31561 aaagtaatta tagatttaga tagtttaagg gattatataa aaagtggata ttatgattgt
    31621 aattgtaaaa atgaaaagta ttctgagttt tcagttgagg atatatatat agatattagt
    31681 gaattggtga gtggaaaaaa tagaatttta tctgataatg agataagcga gatatataat
    31741 tatgcagaaa aagagcttaa ggttttggaa agtaagtgct tttcagaaga agagttaaaa
    31801 aatgcattta tagaaaagtt agataagaag tttaatgcta ttaaaaacaa taaaaattta
    31861 tctgatagaa gaagtcgaat agactgggga ggacaattaa aagagtctac actggagata
    31921 atggataagg taattgtatt tattaaggag cagtttaagc atgtaaatca aggtctgtac
    31981 ttggctctag caattcatat tgagcatgct ataggtagga taaaagatgg aaaaactata
    32041 ataaatccga gtttagataa aataaaagca tcaatgccaa tggaatatga gttgtcaaga
    32101 tatataatgg gtatagtaga agatttgaca caagtaacat ttccagaaga tgagcttgga
    32161 tatttggctt attatataaa taaattttgt tataatgagg aaagtataaa agataaagtt
    32221 aaagtagtaa tagttacgca tggtaaagtt ggaattgaaa tgtctaaagt tgtaaatcat
    32281 attttaggta tagaatgtac actaggtata gaaatagcac ttacagattc accatcagag
    32341 gggatagagc atgtattaga agaacttcaa aagatagaag ctagaaaagg tattctagtt
    32401 ctaattgata tgggttcttt ggtaattctt ggtgatgagg ttgaaaaaag gcttggaatt
    32461 agatgtaaaa ctgtaaatag agtagataca ttattggcaa tggaagcagg aaaacttgca
    32521 acaattgaag gaaaaagttt agatggtata atagctgatt taaagaaaaa taagaattat
    32581 gctatgataa atacaaataa atttagttac agaaaaaacg agtatggaaa gaaaaatgta
    32641 atcattactt tatgtttgag tggtgtaggt acagctttaa atttaaaaga gcatatagaa
    32701 aaacaaatta aggaatatga cactttaatt gaagtaaagc cagttgcttt tttaaataat
    32761 aatttagaag aagagttaaa tgatattgaa tctgaatata atattattgc tatctgtgga
    32821 actattgata tagattataa aaacgtgcct tttatttctt ataatgaggt acttggtaaa
    32881 aatggtatta ataaagtatt agagcattta aatagtaaac aaccagcttt aaatattgaa
    32941 aaccagttga ataatttaat acatgaagat ttgattttat acaattttga aggaatttca
    33001 aaggagtata taattgatac tcttgtttca aaacttgaag aaggtggata tgttgactca
    33061 aagtatatat taagtgtata taaaagagaa gcaatgggaa gtgttgtaat ggcctctaag
    33121 gtagccgtgc cacatggact tccagaaaac gttatgaagc ctgcaatagc aatcgcgagg
    33181 ttgaataaac ctatagtatg ggacaataaa tttatggtgg atttggtggt actactggca
    33241 cttaaagaaa ataataagaa agaaatacga agcttatttt caaagattaa tgaccagcat
    33301 actttagaag ttttattaaa tactgaagat aaaaatgaaa taaaagaact tttaagctaa
    33361 ttattagtac cgagaagaaa gacaaaaaaa tttgctttag tataaaattt aaaactactc
    33421 aattgctaag tagttaatta ctctatataa ggctagttct atgaaattta tttatatgat
    33481 tttatagaat tagctttttt cttggtaaat aaaatatata aatttattta tgt
//
LOCUS       DAYATN010000029        31921 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.029, whole genome shotgun sequence.
ACCESSION   DAYATN010000029 DAYATN010000000
VERSION     DAYATN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.029"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(149..469)
                     /locus_tag="ACGI8A_001346"
     CDS             complement(149..469)
                     /locus_tag="ACGI8A_001346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179349.1"
                     /translation="MKSINELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKK
                     IYETIGINSITKISIDKNKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRF
                     RIKS"
     gene            complement(593..1465)
                     /locus_tag="ACGI8A_001347"
     CDS             complement(593..1465)
                     /locus_tag="ACGI8A_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HFH0179350.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKNIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRGFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            complement(1518..1877)
                     /locus_tag="ACGI8A_001348"
     CDS             complement(1518..1877)
                     /locus_tag="ACGI8A_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFH0179351.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            complement(1931..3016)
                     /locus_tag="ACGI8A_001349"
     CDS             complement(1931..3016)
                     /locus_tag="ACGI8A_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFH0179352.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            complement(3187..4566)
                     /locus_tag="ACGI8A_001350"
     CDS             complement(3187..4566)
                     /locus_tag="ACGI8A_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HFH0179353.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(4559..5239)
                     /locus_tag="ACGI8A_001351"
     CDS             complement(4559..5239)
                     /locus_tag="ACGI8A_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFH0179354.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(5630..6709)
                     /locus_tag="ACGI8A_001352"
     CDS             complement(5630..6709)
                     /locus_tag="ACGI8A_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0179355.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      6997..7112
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7287..8657
                     /locus_tag="ACGI8A_001353"
     CDS             7287..8657
                     /locus_tag="ACGI8A_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0179356.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(8922..9533)
                     /gene="yihA"
                     /locus_tag="ACGI8A_001354"
     CDS             complement(8922..9533)
                     /gene="yihA"
                     /locus_tag="ACGI8A_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFH0179357.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(9523..11886)
                     /gene="lon"
                     /locus_tag="ACGI8A_001355"
     CDS             complement(9523..11886)
                     /gene="lon"
                     /locus_tag="ACGI8A_001355"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFH0179358.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            12066..12578
                     /locus_tag="ACGI8A_001356"
     CDS             12066..12578
                     /locus_tag="ACGI8A_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFH0179359.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            12566..13093
                     /locus_tag="ACGI8A_001357"
     CDS             12566..13093
                     /locus_tag="ACGI8A_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFH0179360.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(13164..14414)
                     /gene="clpX"
                     /locus_tag="ACGI8A_001358"
     CDS             complement(13164..14414)
                     /gene="clpX"
                     /locus_tag="ACGI8A_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFH0179361.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(14433..15017)
                     /gene="clpP"
                     /locus_tag="ACGI8A_001359"
     CDS             complement(14433..15017)
                     /gene="clpP"
                     /locus_tag="ACGI8A_001359"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFH0179362.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(15167..16453)
                     /gene="tig"
                     /locus_tag="ACGI8A_001360"
     CDS             complement(15167..16453)
                     /gene="tig"
                     /locus_tag="ACGI8A_001360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFH0179363.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(16647..17117)
                     /locus_tag="ACGI8A_001361"
     CDS             complement(16647..17117)
                     /locus_tag="ACGI8A_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0179364.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(17117..18466)
                     /gene="rph"
                     /locus_tag="ACGI8A_001362"
     CDS             complement(17117..18466)
                     /gene="rph"
                     /locus_tag="ACGI8A_001362"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFH0179365.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRTIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(18626..20650)
                     /gene="ligA"
                     /locus_tag="ACGI8A_001363"
     CDS             complement(18626..20650)
                     /gene="ligA"
                     /locus_tag="ACGI8A_001363"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFH0179366.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(20836..21774)
                     /locus_tag="ACGI8A_001364"
     CDS             complement(20836..21774)
                     /locus_tag="ACGI8A_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFH0179367.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(21876..22298)
                     /locus_tag="ACGI8A_001365"
     CDS             complement(21876..22298)
                     /locus_tag="ACGI8A_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HFH0179368.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            22486..23769
                     /locus_tag="ACGI8A_001366"
     CDS             22486..23769
                     /locus_tag="ACGI8A_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HFH0179369.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(23847..24362)
                     /locus_tag="ACGI8A_001367"
     CDS             complement(23847..24362)
                     /locus_tag="ACGI8A_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0179370.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(24362..25738)
                     /locus_tag="ACGI8A_001368"
     CDS             complement(24362..25738)
                     /locus_tag="ACGI8A_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HFH0179371.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(25763..26314)
                     /locus_tag="ACGI8A_001369"
     CDS             complement(25763..26314)
                     /locus_tag="ACGI8A_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0179372.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(26372..26587)
                     /locus_tag="ACGI8A_001370"
     CDS             complement(26372..26587)
                     /locus_tag="ACGI8A_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179373.1"
                     /translation="MQIHRELSKSENEYMCTKKEEQRYSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESK"
     gene            complement(26556..27389)
                     /gene="fdhD"
                     /locus_tag="ACGI8A_001371"
     CDS             complement(26556..27389)
                     /gene="fdhD"
                     /locus_tag="ACGI8A_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFH0179374.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(27438..29582)
                     /locus_tag="ACGI8A_001372"
     CDS             complement(27438..29582)
                     /locus_tag="ACGI8A_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(29166..29168),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFH0179375.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(29833..30891)
                     /locus_tag="ACGI8A_001373"
     CDS             complement(29833..30891)
                     /locus_tag="ACGI8A_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFH0179376.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPEKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(31102..31761)
                     /gene="cprR"
                     /locus_tag="ACGI8A_001374"
     CDS             complement(31102..31761)
                     /gene="cprR"
                     /locus_tag="ACGI8A_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HFH0179377.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT         9666 a         5717 c         3393 g        13145 t
ORIGIN      
        1 attatatttt gcttttcatt cttcaaacta gttaggtttt cttccaaata acttactgct
       61 atatcacttg ccaatataat aactaatatt aacaatattt taagtagatt tttttctcta
      121 ttatttaatc tcaaaaataa tcacccatct aactttttat tctaaatcta taatattctc
      181 catctcttac aatactgtct atgtaataat attttatgta ctttattttc cttaaattgt
      241 ttaaaacatc taaatcacta cacacacctt ccatctctag cttattttta tctattgata
      301 ttttagtaat actatttatt ccaattgttt catatatttt ttttatttca ttaagtttta
      361 ttttagaaaa tttctctcta gttttatctg ttgttggtaa agtgttcttt gatttatcca
      421 ctagcttatt ttgacttata taatcttcaa gttcatttat agatttcaca tacataaatg
      481 atttaaataa aatacacgtt aaaattaaga ctattattat tttatagatt ttattacatg
      541 gtttagtttt tttaaatcca ttaagaaaat ttattgtttt atccactttc acttaaataa
      601 tcattcctat actatttata taatttatcc tattgtcttc taaatctgca tcaaaattat
      661 tttctacttt tactttatct tttaatctta atggatttac ttcaatagat ttatctgtat
      721 atttaaagta ttcttcaata aaacaatttt ctataccata gtaataaaca tacttaaaac
      781 cccttaataa tctttttatg aactcatatg attttccctc ttttgatagc aagtatgttt
      841 ctcttatttt tttatctttt gttatattta taattaactg acttttttta cattctataa
      901 ataccccttc ttctgaaaaa ttctctatta aatttaagtt tataagcttt tgtaatatat
      961 caaaatttat atttatagtc tttggattca tatttagatt ttctgaaatg ttcttaatta
     1021 attgaataat attctttggg aaaagaatta cttgtatatt caatttatca tttttagaat
     1081 ttataacttt atattttatt gaatagttat ttatgtttat tggaatgtat tgtgttattt
     1141 caaattcaat catgcttaat atgtctcttt ttttcttagt atttagaact tcaacatttc
     1201 taatgactat atcctgaatt tgaatgttaa agtataaatt tttatataat atttttgatt
     1261 taccaatctc ctcttttaca aatctaacta catcttctat atatttaatc ttctcaatcg
     1321 aattactatc attttttata tcaaagttaa tctcttttat aattgattta tctataaaaa
     1381 cttttttatg actttttctt aaaaatacaa gccttatata atcatcattt atttctaagt
     1441 ttagaatttt tttaattata gccatctcct tcttaaataa aaataatttt aaaacttaca
     1501 gcaatcaata taaaatatta ttttggataa aattgcttag aatttatatt tacgcttata
     1561 tctgttccgc tatcatttat gacaagttcg aatttacctg atgtactctg aggaactgga
     1621 acagtattta ggtatccctt ttccttcaat gtctctacac ttagagagtc tattgtcttt
     1681 tcatcatcat taattgccat gctagctgcg gttactatat tagcagcatt cgtatagtct
     1741 gcatttaatt tggcactttc ttgaacctta ctatacttaa ctaatgctat actagatata
     1801 acgcctaaaa tagttactac tacaatcatt tcaacaagtg taaatccttt tcgtttttta
     1861 tttatcaaat ttatcaatta aatattctct ccttttcaaa agtttcatct aataaaattt
     1921 atttttctct ctaaaaatta gtaattgcat caaacatagg aacaaccatt gctaaaataa
     1981 aagtaccaat aattaatccc ataaatacaa ttagcatagg ttctataaat ttcattattt
     2041 gctctatttt gttatttaat tcattttcat aaaatttatt tattacattt agacttttat
     2101 ctagtttccc actttcttct cctgtatttg ccattgaaat aaataatttt ggaaaaatat
     2161 ttgctaaatt caaagattta cctatgctat ttcctttttt tatataatta ttagaaatct
     2221 ttaacctttc ataaataaat tcgttgtcta taacactagc tgacatatct atagcttcca
     2281 caatttgtat tccactgcta attaaaacgg acaatgccct tgaaaatctt gttgtagcta
     2341 ctaactgatt aatttttttt ataaaaggta ttttgaattt taacgaatta atcaattttt
     2401 ttatattatc atttgttata aagaaataaa tgcttcctat tgaaaacaat ataaaactaa
     2461 atattatata agctaagtta tttgtaacca ctgtagatat tcccattaaa actctagtta
     2521 ttaaaggtgg ttcaattcca ttgttggtaa aaaccacctg aaaattaggt attacaaata
     2581 cgaatataaa caacattgat aagatagata atattataag tattattgga tatattgaaa
     2641 tagttattat tttcattttt aatttgtatt ctttatcata ataattagat aaatcactca
     2701 ttattatatc taagtttcca ctagtttctc ctgcttttat catacttata aagaatttag
     2761 aaaatatacc tgttatttga aatgattctg ctattgagtt acctttttga atatgatttg
     2821 atactatact aagcaagttc tttacttttt tacttgactg actctcaatg atttcaaata
     2881 tttttgtaat ttcacatcca gattccagca atatgcctat ctctttgcaa attactctaa
     2941 tttctttatc actgattcta tttaatacta aataaaactt ttctaaatta taaaatatat
     3001 ttttagataa tctcatgtac tataatacct tccaaattat attaattaca attaaataaa
     3061 cttaatattt tctaatttaa tgcatttaat tttatttcac ttaaatataa tttatactca
     3121 aacatcttaa ttaaaataga ttaaataaag acaactgacc aaacgaagtt taactataca
     3181 attcagttaa atcatttcat ttactactaa acactcttct aatgtcgtta ttttttcatt
     3241 tattagtctt ttacaactat cttcaaatgt tatcattcca ttattttgtg ctacatcttt
     3301 aatagtacta gaatcctcca tatttcttat acatgtcttt atatcatcat taatttctaa
     3361 gatttcataa attgctgttc ttccaaagta tcctttatca ttacattttt cacaccctac
     3421 agctacctta gtatttacat ctccactaaa attatctttt atcataatct catggctaca
     3481 atgattgcag acttttctaa ccaacttttg agatataaca cctataagag aggcatttaa
     3541 aagatatggt ggtatttgca tttctagtaa tctagctatg gaagatactg catcattagt
     3601 atgtaaagta cttataacta aatgacctgt tatagctgct cttactgcta tcttagctgt
     3661 ttctgcatct cttatttctc cgaccattat tatatctggg tcttgtctta atatcgctct
     3721 aagacctaca tcaaagctca accctacttt tgaatttact tgaatctgac ttatgccatc
     3781 catcttatac tctacaggat tttctatagt cataatattt ttatttattc cttttaaatc
     3841 atttaataag gaatatactg ttgtagtctt tccactacct gtaggacctg tcactaataa
     3901 tattcctgct cttttattta ttatacgatt tatagatttt atagcttcct ctgaaaaacc
     3961 aagttctaat ttgtctttta aaaaagtatc tctattaagt atcctcaaaa ctattttttc
     4021 accatagaca gttggtattg atgaaactct aatgtcaatt aaatttccac ctaactttat
     4081 atccacccta ccatcctgag gaagcctttt ttctgttata tccattgatg cctttaattt
     4141 tataaccgta gacaatgatg gatagacatc aactaaaaac tttgatactt cctttagttc
     4201 tccatctacc ctcattctca ctattaaatg ttctttaaat ggttctatat gtatatcact
     4261 tgcgttttct tttatagctt tttctaaaag attttcaaat aatttttttg cataatcctc
     4321 atcaagttca aagctattat aatatttttc aatcttttca cttataagtt cttttttctc
     4381 cttcttcaat ataatttctt tacctgtagc taatctcaag tcctgtagag cataaatatc
     4441 ctcatcttct atttctacaa ctaaattatc atcttgaaac tcaactggaa atacattata
     4501 tttttttgca agtttttctg gtattaacat tggcaaactg ctttcctcta atataatctt
     4561 aatcaccctt tatatacttt aatttattag gtatattcat ttttttcata gagatatttt
     4621 cttcagtttt tatgcttatt ttcattggat aattggtttg tatgctatct aaatatttac
     4681 tacctttatc tacataatta gatagtttct ccttatctcc aatgcacttt atttcatatg
     4741 gttgtgctac aggaactcca tttatattta tatggcttcc tgcaagtaca acttctgaat
     4801 attgattaat tctttgacca tttatttcta tgtattttcc tccgtttact tttaattcat
     4861 ttataacttt tacaagaatc ttattatagt ctatactatt agctatattc cctgtttttt
     4921 catctatagc atctatactt aatattatcc cactaccctt tacatctgaa tatgatagac
     4981 tatctctaag tgtttctatt tctgatattt cttcagaatt tttttgattt tgtttcattg
     5041 aattaatttc tttatctaac ttgtctttct ctttggttaa ttcttttata gattttcttg
     5101 tcatttttat ttcttttctt attgacttgt ctttgcttgt ataaactgtt tcttcattgc
     5161 tataatctct aaatatggtt ccaacaagta tacctaaaat agtacttgct agtattataa
     5221 acacatattg ttttttcata ctgtattgct ccttacaaat ttatttttat atgataattt
     5281 tctttaatta aatattaaac atttacatat gcttattata tcaaagttat atattaaaat
     5341 tcatctttag tatataatat catacattga actcctatca ctaatttacc aatatcaagt
     5401 atgcattttt agatgttctt aaaattatat aaactttcaa taaacattgt tgtgatatta
     5461 taaaaaaaca taaattagga aaggaaataa taattatgaa aaacaaataa tggctttgga
     5521 gattattaat atcatttttt agcaaaaaaa gtaaaatggc atagcctcaa agctacacca
     5581 atttactaaa aattatatta ttactaacgt taattttaaa catatgcgtt tataagtttt
     5641 cctcatattt taaaattctt ttaattaact tatctcttaa tttccctact tcatcaaatc
     5701 tcatattagg actatagtaa ctttctataa tatcatgttt agacttggct ctcttaagat
     5761 ttagtactgc ccaacaaatt aaatcatcta aaactttttc atcaaattta atatcttctt
     5821 catatttaag tagtttttct ctatttaaaa actgatttaa atcaattgta tcttcttctt
     5881 taaaagtcgt acttatagtt atgcctatat ctaaatcagt taatagtaca gtttcaattt
     5941 tctctggaat aagtggataa tgataaactt ctacatttaa acctttttca atagctttct
     6001 tatatacttt gtttaataat gtagttttcc cagttcctat ttctccatct aaataataga
     6061 ctttctttac atcttttaaa atgttttctg tataatctaa acaccctaca ggagttatag
     6121 ctgaaccaaa taaatgcttt tctcttttta gatttcctgt gcttttaact ccatcaaata
     6181 acctttttat aaattcttct gtgaccaaat ttacttttcc ataatccata caatcagaat
     6241 atttttcttc tatgtctcta aagattggtt ctgctgattt taaaaactta aatgctcttt
     6301 taaatgaatt actaatatct ttatcataaa ctgatatttc ttgtttattc ttttctaatt
     6361 cttctgtatt ccagtaatca cctaaattaa ttatctcatc caaagctcca ggtagctttg
     6421 ggtccatagt atgtggggca gttgcatcca taagtaccac gcctaatttc tttataacta
     6481 aaccatccaa cgaacttggg tcagaaggac aatgatataa atcaatatca tatcctctat
     6541 gcaaaaactc tttataaatt tttttcataa gtgaggattt tcctactcca ggtccaccct
     6601 ttaaacaaaa aactcttttt acattttcag gaattatata gtcaaaaaaa gaatatgagc
     6661 cttgtgatgt atttccaccc gggaatgcct tctttacctt tccttccata ttagcgcctc
     6721 ctcataattt acaatatctc ttaattaaga tattcatatt ataagaattt tattacagct
     6781 ttctgatttt gataaattta aaacttacca tctaatacta ataacaggtt aattttatct
     6841 aattattata gattctcata ctgttcctta ttctatctat aaatacaatt taagtgtcca
     6901 tattgaaata tttgtattgt aatacagctg gatattactt aaatccaatt gtttccatta
     6961 taattttatg ttaaaataat attacaaaat acatctgttt ttcttcataa acgggtgaaa
     7021 ttccctatcg gcggtaaaag cccgcgagcc ttatggcata atttggtcat attccaaagc
     7081 cgacagtaaa atctggatgg tagaagaaaa tagtatatga gtacctttat gtaattttac
     7141 atgagtaatc tatacaaatc cttcaactac cgtatttatt catgaaatta gacacattca
     7201 aggtacctaa tatacaggtg ctttttttgt tgtttatttt acaattatat cgtacttata
     7261 aaatctatta agattggagt gttatcatga aacaaaaatg gatagtattg attatcatct
     7321 gtattggtgt atttatgtct actcttgatg gaagtatact aaatatcgca aatcctacaa
     7381 ttgctgctga ctttaaaatt aacatgtcac aaattcagtg ggttgtaaca gcttatatgc
     7441 ttgttgtaac agctactatg ttatttttcg gaaaacttgg cgataaagta ggttcaaatc
     7501 gtctatacac attaggattt tttatattta caataggttc ttttctgtgt agtatgtcaa
     7561 acaatttatc aacattaatt tcttcaagaa tctttcaggc agttggagca agtattttaa
     7621 tggctactgg gcttggtata gtttccaatg catttccagc aaatgaaaaa ggaaaagcta
     7681 taggaatcac tggagctgta gttggtatag gaaatatgag tggtcctgta attggaggta
     7741 taattttaga acattttgga tggccttcta tttttattat caatatacct attggtatta
     7801 tagcagtctt tcttggcatt aaatttctac ctaaacctgt tttggatgaa caaaacaaga
     7861 gctttgatat tccaggtctt ctacttttcg catcttgcac tacgcttata ttacttgcaa
     7921 tgaatgagaa gggtaataca agattgtatt taggtataac agcacttata atcttcttat
     7981 tgttggcatt aagagaagta aaatttgaac aaagctttat tgacctacct ctatttaaaa
     8041 atagaaactt tactgtaggc aatatcattg gagtagcttg ttattttcct caaatggcag
     8101 tatcttttct acttccattt tatttagaac aattaaaaaa tttatctcct atgatggctg
     8161 gttatgtaat gactgtacat ccacttatta tggtacttat agcacctatt gcaggttcac
     8221 tttctgataa acacggtgca aaaaacattc taacagcatc tttttcattt atgaccatct
     8281 cactagttgg aatggcactt ttaaaagcag attctccatt gtatctttta attgtatgtt
     8341 tagttatttt tggacttggg ctaggagcat ttagttcacc aaacaatagt agtattttag
     8401 cagatgttcc tcctcaaaaa cagggatacg gaggcagttt tcttgcaact attcgtaatt
     8461 tatcatttgc tcttggaaca gcatttttta gtagtttttt tgcacaaagt cttacataca
     8521 atcaaaagtt taaaagtcac acatcagcct atgttatagc tagcaatcag tcttattgga
     8581 ttgcagcatc agtatgtttt atagggctta tactaactgt tttctttatg agaaaaacag
     8641 ataaatctat ttcttaaatg ctatttattg ctgactacat aagtcaataa ttttttagtt
     8701 gaactaattt atactaattt tcccatacct tactaaaatt tattagtatt taacaaaatt
     8761 actgatattt attgtttata aacaataagg atgccaaaaa aagttcatta aaactatttt
     8821 aaggcatcct atattttgct ttaatattaa tataattaaa actaactatt attacattta
     8881 ttattcaaat aaatctcatg tcaatattta acattttaaa tttaatctac tgttatttca
     8941 tatccatgtt gattgtattg atttacaatc tcttcccata attcttcaac accagtcttt
     9001 tttagtgaag aaacaggaat tactttttca ctactttcca tttgcaattt ttttcttatt
     9061 atacttatgt gtttttgata ttgtcctcta gatattttat ctgccttagt tgcaataact
     9121 acacagttat atccaaagtg ctttatccat tcatacataa gcttatcatc tgctgtaggc
     9181 tcatgtctaa tatctacaag taggaatata gaacataact cttgtctact ttctagatat
     9241 cgttccatta tacctcccca cttttctttt tcagacttag ctatctttgc atatccatag
     9301 cctggaaggt ctacaaaata aaattcatta tttattagat aaaaatttat agttctagtt
     9361 tttcctggtg tttgactggt tctagcgaag tttcttctgt taagcaatgt atttattatt
     9421 gatgattttc caacatttga cctaccagcc aatgctatct ctgggattcc ttctgctgga
     9481 tactgtgatt tattcacagc actcatagtt atctctgaac ttctaatttt cattattttc
     9541 accactcttt aataaagctt gttctaatac ttcatccata tgttctacaa gcacaaactc
     9601 cattttttct tttacatttt ctggtatttc atctaaatca gcttcacatt ctttaggaat
     9661 taatactttt gttataccag ctctatgagc tgctaataac ttttctttaa ctcctcctac
     9721 tgctaagact ctacctctta aagttatctc tcctgtcatt gctatatttc caggcacagg
     9781 tcttttagta agtgctgaga ttactgcaag agccatagtt ataccagctg atggtccatc
     9841 ttttggaaca gcaccttctg gaatgtgtat atgaatatca ttcgtcttat aaaattcagg
     9901 gtctatatca aacttgtcca ctattgacct tatataagag atacctgtct tcgcagattc
     9961 cttcataaca tctcctaatt ttccagttaa tacaatttct cctttacctt taagtacgtt
    10021 tacttctact tcaagtgtaa ctccaccaac ttctgtccat gcaagaccat ttactaatcc
    10081 tatttgaggg ttaacttctg caagttgata cttaaacatg tcttttccaa gatatgtctc
    10141 taggtcagac ttatttataa caacctcttc aagagttggg tcttcaacgt attttttggc
    10201 aacctttcta caaatcttac caatagttct ttctagtgtt cttacaccag cttctcttgt
    10261 ataatggttg attatacttc ttaaagtttc atcatctatt tttataaagt tttcttttaa
    10321 agcatgttct ttaatttgtt ttggaagtag gtattttttt gctatattta atttttcttc
    10381 ttctatatat ccagatactt ctattacttc cattctatct aataatggtc ttggtatatt
    10441 acttagacta tttgctgttg taacaaatag tatttttgac aaatcaaaag gtatttctaa
    10501 gtaatggtcc acaaaatcct tattttgctc tgggtctaat acttctagca ttgctgaagc
    10561 tgggtcacct ttataatctg ctgccatctt atctatctca tcaaataaga atacaggatt
    10621 ttttgtttgg gcttctttta ccccattaat tattcttcct ggaattgaac ctacataagt
    10681 tcttctatga cctcttattt ctgcttcatc tctaactcca cccaaagata ttcttacaaa
    10741 ttttctattt aatgctctgg cgattgattt tactatagat gtctttcctg ttcctggagg
    10801 accaactaga catattatag gccctttaag tgacttagca agagttctta tagctagata
    10861 ctctagtatt ctctccttaa ctttttctaa tccataatgg tcttcatcta atatatcttt
    10921 tgccttagtt atatctaatt tatcttttgt ctctttattc catggcaatg agaatatagt
    10981 atctaaatag tttctactta cagatacatc tggagacata gaagatattt ttgaaaattt
    11041 atctatctct ttttctattt tctctttagt tgtcttaggt gcttttattt ttttaagctt
    11101 ttctctatac tcatctgctt cagagtttat atcttcttct tcacctaatt ctttctgtat
    11161 agcttttaat tgttctctaa gataatattc tttttgaacc ttattcatct gtttttttac
    11221 tcttaaagtt attttctttt ctatttttaa tatatctatt tcctctaata atatagaata
    11281 aataagttca agtctttttc taatatcaaa ttcttctaat atttcttgtt tttggcttga
    11341 ttttagatat atgtttgcag ctatcgtatc tataaatcta tctacatctt ctatatcagc
    11401 taaagatatt aatatttctg gtgataccct atttccaata tttatatact cttcaaaggc
    11461 atcaaataca tttcttacaa atgcttcaat ttcaacttct gtttcactat ccaaattatc
    11521 actatcaaat actatttctt ctattactgc tctgaaatac ccatcttctt gttctatttt
    11581 ctttactcta ccccttgata caccttcaac caaaacacga actgtatctc ctggtaattt
    11641 tatcatttgc ttaactttac agattgttcc cacatgataa aaatcctctt ctcctggctc
    11701 atctacctct gcttctttct gagaagttaa aaatataagt tcttcatcca tcatagcttg
    11761 gtctaaagct ttaagagata tctctctacc tatatcaaaa tttaatatca tatatggaaa
    11821 tatagctaat cctcttagag gaattaatgg taattcatga tctattttag tataattttg
    11881 ttccatcaac attactcctt ttctagtcta attgctttgc ttttaatatt atatatttga
    11941 acaagttaat tttcaacatt aatgataaat tcttagaata ctatcatact atattatctt
    12001 tttattatga tataatatta atattagtat acttagatgt tatttatctt ttggaggttt
    12061 ctattatgtt gaaattgttt ttaacctttt taaaaattgg tgcgtttaca tttggtggtg
    12121 gatacgctat ggttcctctt atacaagatg aacttgtaaa caaacaaaaa cttttagagc
    12181 aggaagaatt tattgattat ttatccatag ctcaaagtta tcctggagta ctggcagtta
    12241 atatatctgt attattagga tataaacttt atggtatacc aggagttctt atatgtacct
    12301 taggctctgc tttaccatca ttttgtatag tcttagtctt atcgtatcta tactttaaaa
    12361 attcatcatc taaaatttta gatggatttt ttaaaggagt tgcgcctgtt gtaatagctc
    12421 ttatattgta ttcttttacg agcatgttca agaaactacc aaagtctaaa actaacatac
    12481 tactattaat tatagcagtt ttatctgttg gtgtattcaa tataagccct atttatttaa
    12541 taattggagg aggtgtatat agtctatgtc agaaataata ctactcttct taatattcct
    12601 taaaataggg gcatttagtt ttggtggcgg ttatgccatg ctaccattta tccagcaaga
    12661 atttatatct aaatatcact ttataactaa tcaagagttt ttggatttac ttgctatatc
    12721 acaatctaca ccaggtcctg tagcaataaa tagtgctaca tttgtaggat ttaagactgc
    12781 tggcttcttt ggttctctag ctgcaactat tggtgttaca atttttgcat taataggtct
    12841 aactattata tcaaagttat tttctaaatt taaaaataat aaaaaggtag aaaacttatt
    12901 gacagttctt agaccaatta ctctaggatt catattatca gcagcttttt catctgccaa
    12961 agaagttaat tgggatttat atggaataat tgcatttata gcatcatttt atcttttata
    13021 taaaggaaaa attggttcca ttgcaattgt attcgtcttt ggtattctag gtatattact
    13081 cacattttta taataatgat acctatttta tatttagcat attaacataa aaaaaggagc
    13141 ttttaaagct ccttttcatc atcttatgca ctttcctctt gatccttaag tactatgata
    13201 ggatttactg agtcttcgtt cacagatttc tctgttacta taaccttctt tatattatct
    13261 ctagatggta cttcaaacat agtttccatc attacgcttt caactatact tctaagtcct
    13321 ctagctcctg tatttctttc tatggccttt ttagcgattg cacgaagagc accttcttca
    13381 aattctagct caacatcatc taactccaat aactttttat actgttttac tagagcattt
    13441 ttaggttcct gtaatatctg cattaatgca tcttcatcca acaactctaa agtagctaaa
    13501 actggtattc ttcctataaa ctctggtatt ataccatatt ttaataaatc ctctggaagt
    13561 actttttcat acagcttccc taaatctaat tcttttttac tttctatttt agctccaaaa
    13621 cctagtgttt tatctccacc acgtttttga attatcttct ctaatccatc aaaagctcca
    13681 cctaatataa ataatacatt agtagtatct atctttaaaa attcttgatg aggatgtttt
    13741 cttcctcctt gtggtggaac atttgcaaca gtaccttcta atatttttag aagtgcttgt
    13801 tgaacacctt caccactaac atctctagtt atagatgggt tttcagattt tcttgcaatc
    13861 ttatctatct catctatata tattatacct ctttcagctt tttctatatc aaaatcagca
    13921 gcttgaataa gttttaaaag tatattttca acgtcttctc caacatatcc agcttcagtc
    13981 aatgatgtag catctgccat tgcaaaaggt acatttagag ttcttgctag agtttgagca
    14041 agtaatgtct ttccagaacc tgtaggtcct aataaaagta tattactctt ttgaatttct
    14101 atatccttac tactagattt tttactgtat attctcttat aatgattata aaccgcaact
    14161 gataaagctt ttttagcttt ttcttgacct attacataat catttagaat ctccatcatt
    14221 tcctttggtt ttggtaaaga tgtagtatct tcatctatag tatcttctat ttcttcctga
    14281 ataatttcgt cacaaagttc aacacactcg tcacaaatat atacatttgg acctgcaatt
    14341 aatcttctta cttgatcttg atttttaccg cagaatgaac actttaattg tcgtttttct
    14401 tcatatttag acatattaac acctctctct atctacggtc tctttgtaaa tacttcatct
    14461 attaaaccat attctttggc ttctagtgca ctcatgaagt tatcacgttc agtatccatt
    14521 ttaatttttt ctaatggctg accagttctt tcagataata tctcatttaa agtttctttt
    14581 atttttaaaa ttctctttgc atgaatttct atatctgttg cttgaccttg agctccacct
    14641 aatggttgat gtatcattat ttcactattt ggtagtgcta gtctttttcc ttttgctcct
    14701 gctgccaaca agaatgctcc catagaagca gccataccta tacatatagt acttacatct
    14761 ggtttgatgt attgcatagt atcatatata gccattccag aagttatact tcctcctgga
    14821 gaatttatat ataaatgtat atctttgtct gggtcttcag cttctaaaaa taatagctgt
    14881 gctactataa gccctgctgt ggcatcgtta acttgatctc ctaagaagat tattctgtct
    14941 tttaatagtc tagaaaaaat atcataagat ctttctcctc ttcctgtttg ttcaacaact
    15001 acaggtacta atgccatggt ttcatacccc ctttattcta tataatttac agtagctttt
    15061 tttaagctac tgtaaattat tatacgcatt taactaaatt atatttgcac atcattatat
    15121 actaaacatt tcataacgtc aactatatta gttagcgttt attttattaa gctattgttg
    15181 cattatcaac taataaatct attgtttttc ttatttttat ttgacctttt atatcttctt
    15241 tgtcagcatc tcttaaagaa gcttctattt tttctacttc catattgtaa gcagaagcca
    15301 ttttttctaa ctcagcctta aattcttctt ctgttgcttc aattccttca gctttagtta
    15361 ttgcttctaa tactaaagaa gacttaacta attttttagc atcttctttt ctctcttctc
    15421 ttaattcttc catagttcta ccagtcatct ctagtaattg ttgtaatcca aatccttgat
    15481 attgtaattg gtagttaagt tcatttaaca tattatctat ttgatgttgt atcataactt
    15541 ctggtatttc tatttctgta ttttcagcaa ctttttctac tactgaattt ctagtttctg
    15601 catctgctct atttttagct tcttcttcta acttagctct tacatctgct tttaattcat
    15661 ctaaagaatc aaattcacta gtatctttag caaattcatc atcaagtgca gataattctt
    15721 ttacttttac atcatttatt tttacattaa atacaactgg ttttccagct aaatcttgtg
    15781 agtgatattc ttctgggaaa gtaacattaa cttctacttc ttcaccagct tttttaccta
    15841 ctaattgttc ttcaaatcct ggtataaatg tgtttgaacc tataactaag ttataatttt
    15901 cacctttacc accatcaaaa gctacaccat tttcaaaacc ttcgaagtct attatagctg
    15961 tatctccatc ttctaaagct ttgtcttcta cacttactaa tctggcattt ttttcaacca
    16021 tttcttgtag tttagcatca acattttcgt cacttacagt attctcaacc ttagctattt
    16081 ctataccctt gtagtttcct aattcaaact ctggtttaac ttctacatta agaaccataa
    16141 ccaacccatt gtctttactt atttcttcta tatctaaatc tggattgtct attgggtcta
    16201 tgtctaattc cttgatagct tctggataaa cttctgggaa taatatttct atagcatcat
    16261 tgtagaatat accttttccg tattgagtct ctataacaat tcttggagct ttaccttttc
    16321 taaatccagg tatattgtat ttatttctgc ttttattata agccttattt actgctgctt
    16381 caaatttatt gttatctact gttattttta aagtaacttt attaccctct tttttaatta
    16441 gttctgcttt catccttcta atcctcctag tgatatttat gtaatacccg ataacggtat
    16501 tataattcat tctatttata attcaaccac ttaattatat cacataagtt tttttattta
    16561 caagtgttcc attaatatcg ttgtacttat ttgataaaat ttaatttttt gagcgttttt
    16621 tgccattttt ttagataaat acacctttat atgcgtattt cttcaatttt tatgtcatct
    16681 tcttctatat ccataatgac aaaactttta tacgatactc ctcttggaag tgaagtgctt
    16741 ccaggattta tgtaaagtac accatcttta atttctctaa atggggtatg ggtatggcca
    16801 aatataacta tatcagcatc aacttcagtt gccttttttt ctaacatatt agttccatat
    16861 tttacaccat acttatctcc atggcataag aatatggttt tatttgccac ttcaaagaca
    16921 acttcttcct caacagcatc aaagtcacaa ttacctttaa ctgcaataat acctacatta
    16981 gtcatgctat gaatatatct tgaatcagta aagttatctc ctgcatgaat tattaggtca
    17041 cattctttca aatatggtat tgttttttcc atatttttaa tcatcatatg tgtgtcgctt
    17101 actatgccta ttttcattac ttcactaccc tttttataaa ctcttgtttc ataagtttta
    17161 gtgcgtttgc tctatgactg attgaatttt ttataacact aggtatttca ccaaaagtct
    17221 tatcatatcc ttttactata aataagctat catatccaaa accattttta cctttttctt
    17281 caaatcctat cattccttca caaattcctc ttactacaaa ttcttttcca tcaggaaaaa
    17341 ctactgctat tgcagagacg aatcttgcat ttcttttgct cattggtaca ttttgcatag
    17401 cttttaacag tttttttcta ttttcttcat ctgttgcatg ttctcctgca tatctagctg
    17461 aataaacacc aggttttttg cctaaagcat ctacttctaa gccagaatca tctgatattg
    17521 ctataagttt agtctttttt gctattgttc tagcttttat caacgcatta tgctcaaatg
    17581 tttttccatc ttctactatc tctatacctg ctaaatcaac atcttttaaa gagtacactt
    17641 tatattcaaa atcttttaag atttcaccta tttcttctag cttatgagca ttattagtag
    17701 caatcactac ttcattacca taatccatac ccaatatctc atctgatatt tcacctaaag
    17761 cttctctttg aagccttatt aattctttat tccctttttc acctaactct aacaataaat
    17821 ttaaatctgc tctagaaaaa ggtttctctt ctcctgtccc ctgtacttct acaaactcac
    17881 acttatcagt cattattata ttcatatcta cctgtgcttt agaatcttct tcataacata
    17941 aatctagcat gtgtattcca tttacaatac caacactaat tgcagacaca aaatttgtta
    18001 ttggcatctc ttttattgtc tttgctttat ataatttata tattgcttct gcaactgcta
    18061 caaatgcccc tgttatagat gcagttctag ttccaccatc tgcctgaatc acatcacaat
    18121 ctacccaaat agttctttct cctaatttat tcaagtctac aacagacctt attgttcttc
    18181 ctattaacct ctgtatttct tgtgttcttc catcaatttt tcccctagat gcatctctta
    18241 tctttctttg ttgtgttgct cttggtagca tagaatattc agcatttatc catccagtac
    18301 cactatttct taaaaaagga ggaacttttt cttccactga tgcagtgcat ataacctttg
    18361 tctctcccat ttctataaga acagaacctt ctgcatactt tgtataatct ctagttatct
    18421 ttacaggtct tatctgttca ttacttctat cataacttct ggtcattttt tatatccttt
    18481 ctcatatttt ttatttttta taaatttatt tttataattt ttaattttgt caaatattaa
    18541 ttattgtcat ttttcgtaat cttattagct tactgaacta ttctatcatt aattatagta
    18601 gttttctact aaattaagcg actttttaac tctctaatac agcttttact tcatctttcg
    18661 atgataactt taataattct aaaaatctct cttcatctat aactttaatt cctaaatcat
    18721 ttgctttagt aagtttagaa ccagcttctt cacctgccaa aacaaatgtt gtcgatttac
    18781 taacactaga tgtagctttt ccacctcttt tttctatcat atcttttgca tcatttcttt
    18841 ttaatgtagg aagtgtacca gtcaatacga ttttcatacc ttcaaatatt ttttctactt
    18901 tttcacctgt attatccaat gattttgtat ttactcctac attttttaac ttgtttataa
    18961 cgttcatatt tttgtcttcc ttaaaaaact cgacaacgct gtttgccatt atacttccaa
    19021 attcctctaa ttcttctagt tgctcactag ttgcagaaat tacttcatct aaatccttaa
    19081 agttatatgc aagtatctta gctgctttaa ctcctataaa tttaatacct aatccattta
    19141 taagtctcca taaatcattt tcttttgatt tttcaattga atttattaaa ttgtcagcag
    19201 atttttcgcc cattctctct aggtcgacta catcttcctt ttttatataa tataaatcag
    19261 atacatcttg aataattttt tcatttaata gcagtgtaat tatagattct cccaatccat
    19321 ctatattcat tgcatccctt gacacaaagt gtataattcc ccttcttatt tgggctgggc
    19381 aagacatatt gatacatttt acagcagctt ctccttctaa tcttattgtt ggctctgaac
    19441 aaactggaca cttttctggc attttaaaat ctatttcatc tccagtacgt tcttctttta
    19501 taacttctaa aacttgaggt attatgtccc ctgctttttg aactaatact gtatcattta
    19561 tttttatatc tttttctctt atataatctt cattgtgaag tgtagctcta cttacagtgg
    19621 tacctgcaag tctgactggt tcaagaattg ctgaaggtgt tattgtacca gttctcccaa
    19681 cttctactat tatatctact attttagttt tcttcttttc tgctgggaat ttatatgcaa
    19741 ttgcccatct aggacttttg gctgtatatc ccatttcgtc tctttgtttt atattgttta
    19801 cttttattac cataccatct atttcgtagt ctaaattacc cctgttatct gtccagtatt
    19861 caatatgctc aataacttca tctatacttt tacaaacttt ataactctga cttactgaaa
    19921 atcctagttt ttctaagtac tctagtgatt cactgtgact ttctaaatcg tattcttcta
    19981 tattttccat attaaataca aatatatcta atggtctctt tgctgttagt tttgggtcta
    20041 attgtctcaa tgaaccagct gcaagattcc ttggattagc aaataatggc tgttcttgtt
    20101 cttcttgttg ttcatttact tttctaaaat tatcttttga tatatatact tctcctctaa
    20161 ctattagttc ttttttatca tttatcttaa gaggtatgct ttttactgtc attaagtttt
    20221 gagaaatatc ttctcctaca actccatctc ctctagttgc tcctttttca aactctccat
    20281 tgttataagt tagacctaca gaaagaccat ctattttaaa ttcaacaaca tattcaacat
    20341 tttctcccac atattcttga actctcttat caaaatctct caaatcttgc tctgaaaaag
    20401 catttgataa actcaacata ggagttttat gtacaatttg attgaactta tctaatggct
    20461 tacctcctac cctatttgtt ggagaatcaa ttctctttag ttctggatgt tcttcttcta
    20521 aagaaattaa ctccttcatt aacttatcgt attcataatc tgatatttct gggctatctt
    20581 gattataata cctttcatta tggtagttta ttgtctctat aagtttatta atttcatctt
    20641 ttacgctcac tttttgcacc tcttcatatt tttaaaatta aattattttg aaatttccct
    20701 ataattaatt atctcctatt tatatatttt ttagtatatc actttttggg ctttaaattt
    20761 attacaaaaa aaactcttat tagtatcttt aatacgacta ataagagttt ttatttttat
    20821 caaaattttt tatttttaat agcctcttga tatgtctaca acattatcta caggtttatt
    20881 ctccattata tagtgtttca aattattata aaccatatta aatgttctct ctttgttttt
    20941 atctgacacc caagaattat gaggagttac tatgacattt tcgcactccc acaacttatt
    21001 gtccttgttt aaaggttcac tttcaaatac gtctaatgct acccctctaa atttctctac
    21061 acattcttct aaatctttct gattaactgt atttcctcta cctacattta taaatacaga
    21121 accatctttc ataagtttaa acatatcttt gtttactatt ccatctgtac tttttgtaga
    21181 aggcattgct acaactacta tatcacattc ttcaaataca atattcatat cctcagtagc
    21241 aaaacactta tcaaagtatt ttttactact tccatcagta ttgacacccc atatttctac
    21301 accaaaagct tttaatcttt ttgctgcttc tgaagatata gtacctgttc ctaaaaatcc
    21361 aactctttgt ccatacaatt ctgatacaga aaaatccatc ttccattctt tattttgttg
    21421 ttgtttaaaa aattttgcac tatttttata tatttctaaa atatacaata caatactttc
    21481 accaataggt acactatatc cgcctctatt attagctatc ttaatatttc tcttcaatac
    21541 ctcttctttt ggaagttggt ctatacctac actagttgtc tgtatatatt gtaaattttt
    21601 catcttagaa atatctaatc tctcaaatgg attgtatgta actagtacat ctgctgtatt
    21661 tacatcctca ttattttcaa tactatcttc agagtaatgt atgacatcat agcctaattc
    21721 tcttatttta tcaaattttt cttttccata atttattgta aataaaactt tcatctcaaa
    21781 tctcctccat ctacccacat ttaaaatttt ctattctaaa atataatatg atactcaatg
    21841 tacattagtt aaaattacta taagatatct taaatttact tatgttcaat tgtttttcct
    21901 aaagacatag cattacgttc ttgaactaaa ttttctatta aattattcaa tgtccaatca
    21961 atagatgtct ctgttaccat agctgtaaaa ggtctattta ttctcacttt ttttagccct
    22021 tctaaaaatg catttacttg tgtacttgga gtgttattaa atattacaga cttactctta
    22081 atcccatttt cacattcatt tgatatattt ccatctaaaa tgtctcttat tatattctct
    22141 ccatttttat cacttaagat gatatggtct cttattccag caaagttact tacattttta
    22201 attaatgata attctttttt attaaagttt acaagcataa tacacttcct attgtgtatt
    22261 ttatctaggt tattattatt tattttttca aatgacattt tatccatcct ttcatttatt
    22321 tatatacttt aatttacccc atacatttaa aataatcaat ttttatagtt aatttttata
    22381 ttaataccca aattatattt aaatagttag gctatccaaa actaatatat aaaattagaa
    22441 taattagatt aatatctaca aatcataacc taaaggagag ataccatgaa tacattacta
    22501 aacaataaat ttagcattaa aaacttttca aatgcttggg ttatagtact tggttgtatg
    22561 ttaatacaag ctgttccatt tagtattgta gcaagtatac agcctcaatt tataagttat
    22621 gttgtagaag gagaaggctt tagtttagct tctttttctc taatattcac tattggtact
    22681 attgcctcag caattgcatc accttttata ggtgtattat tttctaaagt caatattaag
    22741 atactatatt tattaggttg tataatttct agtattgctt ttgctacttt ttctatatgt
    22801 actgaactat ggcaatttta cactgtatca tgtattgttc aaattggcgc tacaattata
    22861 tctgctatag gaatacctgt tttaataagt gcttggtttg atgaagaaag caaaggaaaa
    22921 gctttgggct ttgcttttgc tggtggttct attggtagta tatttctaca accacttgca
    22981 gttcgcttat tagcaactca aggatacagt cgttcttatc taatatttgg aattatagca
    23041 ttaatagttg gtatacctgt aatattattc atactcagga tgcctaaaga tattaatgaa
    23101 aaggtaaaat ctaaaggtaa aaaagagaaa gcatctgata caatttctcc ttcagtatgg
    23161 ggttatacat taaaagaagc taaaaaaatt aaatattttt ggattatgac tattggattt
    23221 atatttgttg gtatatatgt ttcatcatta tctgtacaat atccaactta tcttagaaat
    23281 tatctaaatc ctattctaat aggaacagta ggctccacat ttgccttatt tgctctcatt
    23341 ggtaatttat ttggaggatt attgtttgat aaattcggtc aaacaaagcc acttattata
    23401 tcttttgtat tggttcttat agcaacttta tcattactat tggtagataa atcttctact
    23461 tttgcatttg tatttgccat tttgaaaggt ctttctgtat ttgcttatat gattggtcct
    23521 gcttatttaa ctggctcttt ctttggtcaa aaggagtttg gtagcatata tggaatcgta
    23581 caattaattt ttgcagtggg atttgcatta ggttcaacat tatttggatt gttagttgat
    23641 aattttggat acagattttc ttggtctatt ataattttat cagttataat tgcctatact
    23701 ttacttatta taacttctat aggaatgaaa aaattgaata aagaaagatt agataaacta
    23761 atgcaataat tgatttttag atatataaat attataataa aaaagatgga gcttttaatc
    23821 ataaataaaa ctccatcttt tttatattac aatatagcta attttctctt atttttaact
    23881 ttactatcta tatcctcatc cttaactata gtaaatgcct tagttggaca aacatttaca
    23941 catgcaggcc cttctggcga ttctatacaa aaatcacact tatttgctgt cattctttct
    24001 ctaaagcaaa gctttccttc atctacatct tttaatgtaa ctatgtctat tgcacctata
    24061 ggacatgcta acatacaagt cttacatcct atacaatttt cttcatttat ttttattgtg
    24121 ttaccaaccc taactatacc accatttgga catgcctttg cacaaggagc atcttcacaa
    24181 tgtctacact gtattggagc acttacttct tttgtcttta tgacagaaag ttttggatta
    24241 aaatttattt catctggatt ttggtaaaaa atatcttcat ctgaatgtgc aactacacat
    24301 cctatcatac aagttctaca tcctatgcat ttatcagggt ctgctataac aaaatagttc
    24361 atcacttagc cacttccttt cctatacaat atgtagtgtg taatagatga tgagattttt
    24421 ctcctaatgg ttctaccaga tattcatcat acagtttttt aatatcagga ttctcatgag
    24481 attttctaat tgctaaatgt tcatcatgca cgtatgttgc ttttgttcta ttttcataag
    24541 ccaattcctt atattcttct agtaaaattt ttggttgacc tccaccactt acacacccaa
    24601 caggacaagt cataacttca ataaaatcta catctaattt tcctttttcc aagtcttcaa
    24661 gaactggtat tacatttttt aatccactta ctactccaac tcttaagtct aagtctccta
    24721 ttttaatagt tgactttcta aaaccatttt ctcctcttac ttgttttact tctacatcag
    24781 gaatactttc tccagttata agctcatatc ctgttcttat agcagcttcc ataaccccac
    24841 cagtaacacc aaagatagtt ccagctccac tataactacc taaaggacta tcaaattttt
    24901 cttctcttaa atttttaaaa tcaattccca tgtcttttat taaataagct agttctctag
    24961 tagttaatac tacatctaca tctctatatc cactagaatc catttcttct ctatcacatt
    25021 catatttttt acatgtacat ggcattatag aaacactaaa tatctttgat gcatctacat
    25081 tatttatctc tgcaccataa gttttaaata ttgcaccacc catctgctga ggtgatttac
    25141 aagaagataa atgattagtt aatttaggat aatttttttc catgaatttt acccaagctg
    25201 gacaacaaga tgtaaacatt ggaagagttc caccttctgt cactctttta attaattcac
    25261 taccttcttc cattatagtt aaatctgctg caaaattagt atcgtagact ctatcaaaat
    25321 ttaaagctct aagtgctgct gccattttcc cagcacctaa gtatcctaaa taatatccaa
    25381 aatcttctcc taaagcaact ctaactgctg gtgcacattg gaccatagtg aatacatttg
    25441 ggtcacttaa agctttcttc accttatcta tatcacatac attgtatgct gcaaatatag
    25501 gttcatttat agactctggt atctctcttt cttgtttttt ctcttgtaaa aactcaaatc
    25561 cttcatctat aactgaggca taagatttac aagtttgaac acattgaccg cacataacac
    25621 atctatctaa atttatctct tgtggctttc cctcttcacc ttcaatagca tttactggac
    25681 agacctttga acattctcta catccagtac ataaatcctt atctatattt attatcataa
    25741 acgtcaccta cccctgtatc ctttatttat ttgttaacaa taaactaagt gcagcttgtc
    25801 tattcttagc ttttttgtca tctattggag taacaagttt taatgcttcc tgaggacatg
    25861 cacttataca agctattttt tcattgtctt tacataagtc acatttataa gctatcttct
    25921 tattttcatc taaaattact tgttctactt ctttaccatc ttcatattgt ggaagtaagt
    25981 ctatagctcc aaaaggacat gctagcagac aagttttaca tcctatacac ttttcttcat
    26041 ttacagacat aatattgttt tctttaacta tagctttttg tggacatgag tttaaacaag
    26101 gagcatcttc acaatgtcta cactgtattg gcatacagaa attgtcacct tttactaagt
    26161 aaagtctagg tataacaggt atatcaactg ttccaactgt ataacccaca ttattatgct
    26221 gattatgcat tgtaaaacaa gctacttcac aagctctaca acctgtacat ttgtttgggt
    26281 ctgctattac aaaagagcct attttatttt tcatattatc ccacctttca atttgtatat
    26341 ttccaaccct ctagttattt tcatcatatg tctacttact ttcatcatat atatttatat
    26401 atttcactat tttctcagta cttatatttc gtttacattt tttgcacaaa ttgtatgtgt
    26461 tattatacac cttatcagta tcatcaatta atactccaca gatttcacac tttgaatatc
    26521 tttgctcttc ttttttagta cacatatatt cattctcact ttttgataac tctctgtgga
    26581 tttgcataaa tattcatccg tcttcctctt acaaatccaa taagcgttat atttaatttt
    26641 ttagaaagct ctattgataa attagtcggt gctgattttg atactacaat aggaatttga
    26701 gtcatagctg cttttaatat catttccaat gatattcttc cacttacagc cacaatttta
    26761 tcttttaatg ttatgtcatt taaaacacaa aatccaatag ccttatccat tgcattatgc
    26821 cttgctacat cttcacaagt gactattttt ttatcaaaat taaatacaga tacgctatgt
    26881 accccaccag tatttttaaa taattctgat gaatttaaat ttttatccat tatactgtaa
    26941 atcgtatcat aatttatttt tatattgctc tttacaggag cacatgttat atagtctagt
    27001 gaatttaaaa atataatttg ctcatcttga tttttttgtt tctcttgaat agttactttt
    27061 gcaaaacgtt cactctcatt tatctcaaat tttaataagc tttttttgtt ttctatgtat
    27121 ccttttgttt tcaagaatcc tactataagt tcctctaaat tatatggggt acataaaaat
    27181 gtatttacat atttatcatt taaaataaaa ctaagtggat attctgcaat tatttcttca
    27241 ttatctattg atacttcatg ttcatttatt ttgcttactt ctacattaaa agaatttaaa
    27301 tatttgtagt ccactagact ataactattt tcaaaaactt tatccttact attttcttgt
    27361 aatttagttt ttgagtatat attgttcata aaatattagc ctttctgcct tttataaatc
    27421 ttaaatctaa taaatcatta gatatttgta gctgtcattt tgtctttcaa actttgatac
    27481 tcttctctta cacatttttc tgctaattct tggtcttcaa gtttttcaag tttaacagca
    27541 caatatttat actctggtgt tttagatata gggtctaaat ttgctattgt aagttcattg
    27601 caagctccaa cccaccactg ataagtcata tatgttgcac cttccttaac tctatcagta
    27661 actgttgctc ttgttattac acttcctctt cttgaactaa ttctaactaa ttcatcatct
    27721 tcaattccta atttttcagc atcattagaa tttatttgaa cacgtcctgg ttcatcttct
    27781 aatgaactaa gtgttctaca attaccagtc attgttctta ctgaatagtg tccaacttct
    27841 cttacagtac ataaactaaa tgggtattca tcatcttcaa cttccatagg gggtctccat
    27901 tcagcagcga acaaattacc tttaccatta ggagtagaaa acttttgacc ttcatacaga
    27961 tacatagttc ctttatcttc catactttca ctcttacaag gccattgaac gcatccttga
    28021 gtttctatct tttcataagt agcacctaag aaacttggac ataattgtct aagttcgtcc
    28081 catatttcct tagtattttt ataattcata ggatatccca ttgctgtaga aatttcgctt
    28141 ataatttgcc agtctggttt tacatctcct tgtggctcta tagcttttct catcaattgg
    28201 aatcctctat ctgcacatgt atatactccc tcatgttctc cccaagaagt agctggaagt
    28261 ataacatcag catgaagtgc tgtcttattc ataaatatat cttgaacaat gacaaattcc
    28321 aattcatcta atgcttctct aacttctgat gcatctgggt cactttgcac tgggtcttct
    28381 ccaaatatat aataagcttt aagctttttc tcttttagta ctaaatttgg aacttgagtt
    28441 aaactataac cattattagg agataatttt accccccaag ctttttcaaa cttttctctt
    28501 atcttatcat cagttacatt ttgatagcca ggatatacat taggaagagc tcccatatca
    28561 caagctcctt gaacattatt ttgtccacgt acaggtccta ttccaacgct ttctctacca
    28621 aaattacctg taagtagagc aattgatgca agtcccttaa ctacatcaac agcttgacca
    28681 aattgacaga cacccattcc ataaagtatc atagcttttt taccttttgc atattctctc
    28741 atagcttttc ttataagctc ttctgggatt cctgtaatct tttcagcata tttagctgta
    28801 tatggtttaa caatttcttt atactcatca aatccttgtg tatgattttg aacaaattct
    28861 ttattgtaaa gaccttcttc tattaataca tttccaaaag catttactaa aaccatgttt
    28921 gttccacctt ttataggtag atgtatatca gcaattcttg cagattcagt tactctaggg
    28981 tcagtaacaa ttaatttagc accatttttt ttagctttta ctattctatt tgccactatt
    29041 ggatgtgaat ctgctccatt atatccaaat ataaataaaa catctgcatt ttctatctct
    29101 ggtatagaat tagacattgc accactacct aatgagtaag ccagaccggc tacagatggt
    29161 gcatgtcaga cacgagcaca atggtcaaca ttattagttc ctattgtagc tctcataaat
    29221 ttttgcatta tatagtttgc ttcattacca ggtcctcttg cagaacctgt tcccataata
    29281 gaatcaggtc catatttttc tttaatttca tttagtcttg aagctgtata gtttatagcc
    29341 tcatcccatt caacctcttc tagtacacct tgttttctta tcattggctt ttttagccta
    29401 ggtgttagaa ttttagggtc atttagaaaa tcccatccat aacgaccttt taaacacaaa
    29461 ctttcttgat ttgttctacc attagccggt tcagctctta atatcttatt atcttttact
    29521 actagatata attggcatcc agctccacag tatggacaaa ctgttaaaat ttttttctcc
    29581 atacaattcc ccccttagat ttatcccctg ataagtaaaa tttatcttaa agataatgag
    29641 tgtttttata atatgcaaaa tcataaatat tttgcaatac tatctattaa attcaacaat
    29701 tttctcacaa ttcctcttaa atttcaaaaa aaatatgtca taataatctt tatatttcaa
    29761 ccatttaatt aaaatattct agttcttatg acatattttt taatatactc aatatatatt
    29821 ctttaaactt atttagtttt cacttaaaac aatatttctg ccaattctgt ttttcattct
    29881 atagtgagta attacagtaa atattccact tccaatcaga atcaaatagt acataaatcc
    29941 accatataaa aatactgcat atcccatcaa aggttgaggg aatactgatt tgaatatagt
    30001 gaaaaaggct aattcattag ctcctacatt cccaggtgtt ggtattggag atattgccat
    30061 atataaaaat gcttgtaatg tcagtatata aatatagcta taaccttgta aattaaatgc
    30121 tttatatatc caaaatgaaa cactaaagta agcacttaat tgaataaaag ttactattat
    30181 agttgaaatt aatacctttt tattctttgc aaaaaaattt attgctttac tataatcatc
    30241 tataaaactt tctatggatt caatcttatt atctaaaaat tttaaaaact taatttttga
    30301 taaatgcttt atagtaattc tcataaaata ttttattttt tcaggattta aaataactaa
    30361 tattatcata attaagacaa atgaattaat ggctatccct agaaacacta gaggcataat
    30421 cattttcatt tgtttttcta acatagcaaa attcattaaa acaagtattg tacaataaaa
    30481 agtaactact atttggaaga taatagactt attggtaaca actgcacttg ccttactaag
    30541 tggcatttta cattttttaa gtacatatat ctgtactggt tggcttcctg aagcaaaagg
    30601 agttattaaa ttataataaa atcccataat ggctagctta aaaccaataa atctaatatt
    30661 aactttgtga gtgctttcta taattatctt cattactact ccttcaagca ttatgtgaca
    30721 cattattgcc aaagcaccta tgaataaaaa cttcttatct accataacca taacatttga
    30781 tagcatttta atatccaatg ttttaaacac taggtaaatt gttataccta ttaacaaaaa
    30841 taaaaatgta tactgtagta aactctttct aatattcttt agtctatcca ttagaaataa
    30901 ccatcctttg ctcaagattt atactaaata ctatattatt agtatacact atgaatctta
    30961 atattttata acaaaatacc ttacaatttt gtcatttaca aaaaatatta atctataaat
    31021 taaattttaa tatatagatt aatattttac aaacattttg tagatatata taatattttc
    31081 actaaacaac acttatactt attacaccca tttataccct atcccccata cagtttctat
    31141 tgtataaata tcatattgtt ttaatttgct tcgtatattt tttatatgct ctgtgatagt
    31201 acttatatca ctttctccat caaaaccata taccgactca tatattcttt cttttgaaaa
    31261 tacttgtccc ttattttttg ctagaaattc acatattaga tattcacttt tagtaaaatt
    31321 aattttttta ccatcaattg aaacttcttt gcctaaaaag ttaaattgaa catttgagat
    31381 agttaataaa ttcttttttt ctctactttc tcgccttaaa tgagcagtta tcctagccct
    31441 taattctcct acaccaaaag gtttggttat atagtcatct gcacctatac caagaccgta
    31501 cataatatca ctttccatat tttttgctgt caaaaacagt attggacaat caaccttatt
    31561 tcttatagtt ttacatactt caaaaccatc catcttaggc atcataacat ctaatattat
    31621 tagattaaaa taattaaaat ccatgttcat agctttttct acatcactaa ttacctttac
    31681 ttcatgtcca tctcttttca acacattttc aatcaacttt aacatatcta actcatcatc
    31741 tactactaat atcttagaca taataactcc tccatttatc cgtttaatag tattctaatt
    31801 tatatattta ataatatctt taaaattcac attaaccata atactctcat attatagctt
    31861 acaataatta attactattt tacaaataaa atatcttaga tacaaaaact ttatatttca
    31921 a
//
LOCUS       DAYATN010000030        31813 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.030, whole genome shotgun sequence.
ACCESSION   DAYATN010000030 DAYATN010000000
VERSION     DAYATN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31813
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.030"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(68..1321)
                     /locus_tag="ACGI8A_001375"
     CDS             complement(68..1321)
                     /locus_tag="ACGI8A_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFH0179378.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYSKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANNKGLEKNLFMYCDSAEPDRIKMWKSAGY
                     EAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(1299..1991)
                     /gene="terS"
                     /locus_tag="ACGI8A_001376"
     CDS             complement(1299..1991)
                     /gene="terS"
                     /locus_tag="ACGI8A_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFH0179379.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPKETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(2061..2570)
                     /locus_tag="ACGI8A_001377"
     CDS             complement(2061..2570)
                     /locus_tag="ACGI8A_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179380.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(2591..3565)
                     /locus_tag="ACGI8A_001378"
     CDS             complement(2591..3565)
                     /locus_tag="ACGI8A_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179381.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFDKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(3868..4104)
                     /locus_tag="ACGI8A_001379"
     CDS             complement(3868..4104)
                     /locus_tag="ACGI8A_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HFH0179382.1"
                     /translation="MPNFGTTFVTEEAVYNMSFRSNKAEAKLFTKWVTKALKQIRIHG
                     YYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVY"
     gene            complement(4589..5068)
                     /locus_tag="ACGI8A_001380"
     CDS             complement(4589..5068)
                     /locus_tag="ACGI8A_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFH0179383.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMIKELGVYLFGIDGLKLTV"
     gene            complement(5061..5198)
                     /locus_tag="ACGI8A_001381"
     CDS             complement(5061..5198)
                     /locus_tag="ACGI8A_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179384.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVKYCSRSCSKRARRSDLS
                     G"
     gene            complement(5222..6592)
                     /locus_tag="ACGI8A_001382"
     CDS             complement(5222..6592)
                     /locus_tag="ACGI8A_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFH0179385.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCALTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKDGAENA
                     IHNKIKDICISLKAEDYLNMPKKIDNKIEIHLDSKILKYYKELEKEKILELEKDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEALENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(6541..6867)
                     /locus_tag="ACGI8A_001383"
     CDS             complement(6541..6867)
                     /locus_tag="ACGI8A_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFH0179386.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(7149..9566)
                     /locus_tag="ACGI8A_001384"
     CDS             complement(7149..9566)
                     /locus_tag="ACGI8A_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFH0179387.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIETFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDAGVKKTNKSIFNGQLDNERNQIWAEAVELYKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITKNWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(9578..11536)
                     /locus_tag="ACGI8A_001385"
     CDS             complement(9578..11536)
                     /locus_tag="ACGI8A_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFH0179388.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTSISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWLAGEKWRLDVFNSHGKIYEASASQMFKIPIENIKKGSELRQKGKLAELSN
                     GYGGSVGALISMGAIKMGLKEEELQPIVTAWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VEIQHGIKFIYNPGVLFIELPSSRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(11536..12108)
                     /locus_tag="ACGI8A_001386"
     CDS             complement(11536..12108)
                     /locus_tag="ACGI8A_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFH0179389.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(12131..12703)
                     /locus_tag="ACGI8A_001387"
     CDS             complement(12131..12703)
                     /locus_tag="ACGI8A_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HFH0179390.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(12756..13091)
                     /locus_tag="ACGI8A_001388"
     CDS             complement(12756..13091)
                     /locus_tag="ACGI8A_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179391.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     ILNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(13106..14272)
                     /locus_tag="ACGI8A_001389"
     CDS             complement(13106..14272)
                     /locus_tag="ACGI8A_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFH0179392.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPSTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(14284..14721)
                     /locus_tag="ACGI8A_001390"
     CDS             complement(14284..14721)
                     /locus_tag="ACGI8A_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179393.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(14792..14941)
                     /locus_tag="ACGI8A_001391"
     CDS             complement(14792..14941)
                     /locus_tag="ACGI8A_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179394.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            complement(14922..15314)
                     /locus_tag="ACGI8A_001392"
     CDS             complement(14922..15314)
                     /locus_tag="ACGI8A_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0179395.1"
                     /translation="MDISESITKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIAES"
     gene            complement(15345..15542)
                     /locus_tag="ACGI8A_001393"
     CDS             complement(15345..15542)
                     /locus_tag="ACGI8A_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0179396.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELDIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            15618..15992
                     /locus_tag="ACGI8A_001394"
     CDS             15618..15992
                     /locus_tag="ACGI8A_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6173 family protein"
                     /protein_id="HFH0179397.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(16002..16211)
                     /locus_tag="ACGI8A_001395"
     CDS             complement(16002..16211)
                     /locus_tag="ACGI8A_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179398.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMSKSEKRKISEITKERIRK"
     gene            complement(16258..17073)
                     /locus_tag="ACGI8A_001396"
     CDS             complement(16258..17073)
                     /locus_tag="ACGI8A_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFH0179399.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKREKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFEEMEQVLKNEQPKLPTTYKEALQHLIEQVEVNEKLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHS
                     ETIDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKEYSYQTSILG"
     gene            complement(17076..17297)
                     /locus_tag="ACGI8A_001397"
     CDS             complement(17076..17297)
                     /locus_tag="ACGI8A_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0179400.1"
                     /translation="MNNLKELRTERNLKQKDVALKVGITTSYYGMIESGTRVPSLAIA
                     IKLSKCFGVSIEKIFLNTNTTRCCLKERS"
     gene            17450..18013
                     /locus_tag="ACGI8A_001398"
     CDS             17450..18013
                     /locus_tag="ACGI8A_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0179401.1"
                     /translation="MSILSDRLKFLRKEKGVMQKEIANYLNITTSAYGFYEQGKRTPT
                     PEMLSSLAEYFGTTVDYLIGRYDNKASNISSKTSCNNTLFQKRLKELRAEKNMTQEDV
                     ANKLNLTKSAYGYYEQGKTVPDAYMLSSLAEIFNVTTDYLLGRSIVKNDIDTVAAHRV
                     NPHKDLPEEAQEQLNDYIEFLINKYKK"
     gene            18037..19626
                     /locus_tag="ACGI8A_001399"
     CDS             18037..19626
                     /locus_tag="ACGI8A_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFH0179402.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IAFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            19889..20395
                     /locus_tag="ACGI8A_001400"
     CDS             19889..20395
                     /locus_tag="ACGI8A_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFH0179403.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            20635..21765
                     /locus_tag="ACGI8A_001401"
     CDS             20635..21765
                     /locus_tag="ACGI8A_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0179404.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITVEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLNNKTITVRHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(22126..23814)
                     /gene="ilvB"
                     /locus_tag="ACGI8A_001402"
     CDS             complement(22126..23814)
                     /gene="ilvB"
                     /locus_tag="ACGI8A_001402"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFH0179405.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(23867..24913)
                     /gene="ilvC"
                     /locus_tag="ACGI8A_001403"
     CDS             complement(23867..24913)
                     /gene="ilvC"
                     /locus_tag="ACGI8A_001403"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFH0179406.1"
                     /translation="MSNKNKYHKNKKKYWEEMKMARMYYEKDVDLEVLKNKKVAVLGY
                     GSQGHAHAQNLRDNGVHVVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPD
                     EKQKKVYEESVKDNLKEGQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVF
                     TKGSGVPALFAVYQDYTKKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVL
                     CGGISELIKLGYKTLVDAGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEY
                     GDYVSGKRVITDVAKQGMQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIA
                     EVGRNLRSMMSFLK"
     gene            complement(25166..25405)
                     /locus_tag="ACGI8A_001404"
     CDS             complement(25166..25405)
                     /locus_tag="ACGI8A_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFH0179407.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     misc_binding    complement(25518..25775)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(25936..26697)
                     /locus_tag="ACGI8A_001405"
     CDS             complement(25936..26697)
                     /locus_tag="ACGI8A_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFH0179408.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     gene            complement(26702..27523)
                     /locus_tag="ACGI8A_001406"
     CDS             complement(26702..27523)
                     /locus_tag="ACGI8A_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HFH0179409.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            complement(27565..28320)
                     /locus_tag="ACGI8A_001407"
     CDS             complement(27565..28320)
                     /locus_tag="ACGI8A_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFH0179410.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLASLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            complement(28363..29232)
                     /locus_tag="ACGI8A_001408"
     CDS             complement(28363..29232)
                     /locus_tag="ACGI8A_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFH0179411.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            complement(29244..29726)
                     /locus_tag="ACGI8A_001409"
     CDS             complement(29244..29726)
                     /locus_tag="ACGI8A_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0179412.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            complement(29778..30734)
                     /locus_tag="ACGI8A_001410"
     CDS             complement(29778..30734)
                     /locus_tag="ACGI8A_001410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFH0179413.1"
                     /translation="MNKKLYIGMVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVL
                     SATNNYINGQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVD
                     SKYQELEDKMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDM
                     EAYYTSHKKDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESF
                     ESLAKKYSDDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVK
                     VTDKRERQKSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            complement(30803..31636)
                     /locus_tag="ACGI8A_001411"
     CDS             complement(30803..31636)
                     /locus_tag="ACGI8A_001411"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0179414.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
BASE COUNT         9923 a         5693 c         3307 g        12890 t
ORIGIN      
        1 gtgctctctt tttttaactg aacttttatt aaatctgttt cacttatata aatatttaac
       61 gcctccttta ctttaagaag cttattccat tatttttaag cttattatct atagaatatc
      121 taagcgcagc cattgcatca tccataaact caactggttc atcaagatat aatccagttc
      181 tttcatcttg tttccatttc cattgttgta tttcttttat ggtattagtg caactaggat
      241 gtacatgtat tcttaattgt ttcaaataat ctatttgagc tttaacactt cctggtcctt
      301 ttttaactcc tttagcttca tatcctgcac tcttccacat cttaattcta tctggttcag
      361 cactatcaca gtacataaat agattctttt ctaaaccttt attatttgca atctttatga
      421 tttctgaggt atccatttca tgtacatata tttcgttaca tatatataat tctccatcct
      481 taaagccaat tctaagtact acatttgcat ggttaaatcc aaagtcttgt gataacctca
      541 tattgtcaaa atactcaaat tctgtaggaa attcatgtat aacataattt ttaagtattg
      601 ctccaccagt ttctccccat tctccaagac catagacttt gtacccttct gggtcttgct
      661 cttttctcat ttgcattctt ctgtagtaag cctcatctat gaatctattt tgtagataag
      721 tactatgatg agtaaatata tcatcatttt tatagtcaaa atactttctt tttatccaat
      781 gagtagctga gactggatta aatgtaaatg tcatttgata gtataggtta ggattagtta
      841 aaatacctct taaacggtca tctagtatgt ctatgtcact ttccataagt tctgtagctt
      901 cttcacacca aacccatgtt aattttcctt tcgagaagtt aattgatttt aatttttctc
      961 tttgttttgc atcattaact cctctgaaaa ttatagagtt accagtaact ttactcttaa
     1021 tttctaaagg atttaaagta gttttccaat acttatcagc ttgtttacta taaatacgat
     1081 ttatagctcc tgtaagctct gcatacgttg aatacttatg tgtagcttct gactttctaa
     1141 ctactaatag attagctcct tgatacttct tatctcctaa ctttagtata tagtcttgtg
     1201 ctacattaac agattttcca ctccctgctg aacctttcat tgctctgtat ctttttttag
     1261 taaaattagc ttccttgaaa tctggattaa aatttactct aactatcatt tctatcacca
     1321 taatctacac ttattttcaa ctcatcatct ccaatatcat ctttacttag gttatcaact
     1381 tcacatttca acttctcaac tcttgttttc tgctcctctg tagccaaatt ccaatcctta
     1441 tgaatcattt catcatactg tttaattaaa cttctaagtt cactcatagc tctactctgt
     1501 gcattaagaa aagatgcttg cctatcccat gcaaattgaa attcatactc tatcttttca
     1561 ccattttctg tgctttcata tttctttaat tctttaacca tttcttcctt gcctttaaca
     1621 tacattatct tttgtgctct tattattgct gcatattgaa ttgttatctg ttcccaaaga
     1681 atatcaaatt tatcttttat agatatttct tgtatcaatt ctctagtttc tttgggtaga
     1741 tactttgaga agaaaccaaa cttttcagca ttcttattct cttttggagc accatgacca
     1801 actgaatttt tattagaaaa gggtgcacct cttttattta taggtgcacc cttctttttt
     1861 tcactagccc agttgtatct ttttatccat gactttaaag tgtttaaact aatgtcatac
     1921 tttgatgata tttccttttg tttcattcct tttaagtaat cttgttttac cttttctttg
     1981 acatcttgca catcaccacc tctttatttg tttgttttgg gaataaaaaa acaatcctat
     2041 ttaataggat tgtttcaaat ctagtctcct ctatccttcc aggataaatg aaacatactt
     2101 attattaatg tagttagaga taaaattata tgaactgtaa aaaatattaa aacatagaaa
     2161 attatattag aataatatcg agtaagcatt attgtattga taaaacatat tacaacaacc
     2221 aaaatctcca ttattacagt ataatatgct tttaactgta tgaattgaaa gtatgatata
     2281 ttaaatccat ttacctttct ttctgttttt atatcttttg cttctgatat tgatttgtta
     2341 tatgtagtaa acaataatgt aataatgcaa actccaaaag ctactaatag tcctgacaaa
     2401 gttagtacat ctttgtttaa agaaattaca acttctttat taattgacga attaaaaaac
     2461 atgtatgaaa taatacatat taatactggt gttaaaacta actctactac ttctttagaa
     2521 tttatagcct tgtaataatt gagtaagata tttaatacct ttttatacat tttaacacct
     2581 ccttcgcaat ctatgcttca ctcatagcaa cttcttgata cattaaatta aaaaaaactg
     2641 aattattctc aattaaatca tctatcaaat tattcatctt atcaaaaata tcttcactat
     2701 ctatatgatt atctatatca agagaaacct ctatttctct acacaacttt ataccctcag
     2761 tatccaactt taattttctc ttgtttttct ctccctcaat tattatccta tttatttttt
     2821 tatttctaaa ttcctctcct ttaatatatt tttcgcaaca tcttttaact tcactaggta
     2881 aatatctagt tctaaatgtg ggcttatata tcaaatcgtt atgtcgtctt gaattattta
     2941 aattagaaaa caaattatct tcatcagttc caaacttctc tctatcaaca tttaaagtta
     3001 tttttgaaac accatctaaa tctaatattt gttctataaa atctttactt gctatttgtg
     3061 taatattaat tgatgttttt gacatattat tatcaaattt attattttca atcatatcct
     3121 cataatatag atgtaagttt ttacttagtt caatcatggg aacccccaca aaaactcttt
     3181 caaataatac tactgctata ttattttcct tcataaattt aataaatagg tgctgcttat
     3241 tgctatctcc ttgatccatt tttttattac cctctacttc tttagtatga gcatttataa
     3301 tatttgtatt ctgattgtat ttagtatatt caagtttcac cttgattatt aaatcatcct
     3361 cgctacattt tacttctgta atccaaaact ttttatcttt agtaatatta attccatttt
     3421 tgttttcttt acaatattct ttatccttaa agaaggtctt tattacctct tttaaaattt
     3481 cttcatctga atagacactc ccattaattt tatttataat atctattcta taaacatcaa
     3541 tatatctttt tttaaaatta ctcattatac cattattcct ttcacaaata tttataatga
     3601 tataattcta tataatcagt caatatcctt caataatcat tcgacaacca caaaataagt
     3661 ctaaataata cttttacctt attcctgtct ataatgtaca aatatatttc atttttaatt
     3721 cattacataa aaaaaagacc ctccattaat acagtcattt aaatcatttc tattaattct
     3781 ttaatctttt atatacctct ttataattca tacttttatc tatcaaccta ggtgatttca
     3841 tagatataac tctttcaagt gcttgtatta ataaacaaac tcttgtattt catctgtaaa
     3901 atcttttctg acctttttac cttctgttct tatatccagc cattcttgat ctttttctgt
     3961 ggcaatatag taaccatgta ttctaatttg tttaagtgct tttgtaaccc acttcgtaaa
     4021 tagttttgct tctgctttat tacttctaaa tgacatattg tacacagctt cttctgtaac
     4081 aaaagtagta ccaaagttag gcaatttatc tttaaagttt ctagtgtagg aatctccgac
     4141 agtagactca ttaatttctt tttatattct ctatctatat ttcttaatgt atcacgaata
     4201 tttactatgc ctagttcctc tcctacgtca tttgcattaa atcaaacttc ttctccattt
     4261 tttgaccata tcatccttac atttttctct tgtaaaattt tcaacatact actacctcct
     4321 aaattagtta ttaataaaga atattcaatt tgaacaccct tattttcgac ccttcaatat
     4381 gagggattaa aattgtataa aaaagaccta gaaatcactc taagtcatga attactcatt
     4441 tatcattctc cttatttaca aaataaaaaa gactaagttg gggtaactta gcctttttca
     4501 agggggaata tattatacac ttgtttcata ctaccattat aacagggtta aaatatcatt
     4561 aaaatatcat ctttttatca tatatttatc aaacagttaa tttcaagcca tctataccga
     4621 ataaatacac tcccagttcc tttatcattt cattaatcca acgtcttgat gtaactactc
     4681 cacaatttaa tatctctgct atttcttcat atgttttttc atcacaatag taaagctcta
     4741 aagctctata cttttcataa gaatgtaaac gctcttgtct taatttcaaa gttttcattg
     4801 ctgaatctat atgtgctatc ataataatag ttcttgcttt acttttcttt atacttaaaa
     4861 tatacaactc ttcattatca agcttaccta aatcatcctc taagtaatct acatctttag
     4921 cttcacttac cgctgagtct atatgtctct taaaatcatt ataatgcttc atcaataatc
     4981 tagtattata taaaacgcct tttttcctat cttctctttc ttcttttctt aactctctta
     5041 caatttcttt tatactttct ttatccactt aaatcactcc ttcttgctct cttcgaacaa
     5101 cttctactgc aatacttaac tttagattta tccaacttat aaaatcttct accacaccaa
     5161 gcacatctta ctattttacc tgggcttata agttccatct tttcttttta ctttcatacc
     5221 ttcactccct atattctttt aattttgctt tgactgcttc aagtaatgca ctttgtcctt
     5281 tatctttatt ctctagagcc tccattactt gttcatctat tgtaccttta cagattagat
     5341 gatggataat aacagtttcc ctttgcccct gtctataaag ccttgcattg gcttgttgat
     5401 acagttctaa gctccaagtt aatccaaacc aaacaattat acttccacct gattgtaaat
     5461 taagtccatg tcctgtactt gctggatgac aaagtaataa ttgtattctt ccattattcc
     5521 aatcatatat atcttttgag ttttctattg ttcttggttt caaactctta aactctttca
     5581 ttaaacgatt ataatcatgt ttatagttat aaaaaactat aataggctta ccatttgaaa
     5641 catctataat ttcttttaaa gcttctaatt tttctctatg gagttccttt acatttttat
     5701 cattatcata aattgctcca ttagctaatt gtaaaagttt atttgctgct acagctgctg
     5761 atgaagctgt tattatatct ttttctaatt ctaatatctt ctctttttct aattctttgt
     5821 aatatttaag tattttacta tcaaggtgta tttcaatttt attatctatt tttttaggca
     5881 tattcaaata atcttctgct tttaagctta tacaaatatc ttttatttta ttatgtattg
     5941 cattttctgc tccatcttta ggctgccaat tatatatagc tcctgtttga taattttttt
     6001 gtcctggctc aaagtatcgt tctttatatc ctgtaatagt tttacctagt ctttcacctc
     6061 tatcaagtaa gtacatctgt gcccataagt ctattaaact atttggtgtt ggtgtaccag
     6121 taagcccaac tactctttta gttaaaggca atactttctt taaactttta aatcgttgtg
     6181 ctttatttga tttaaaagaa ctaagttcat ctatcacaac catatcaaat ggccattttc
     6241 ttttataaaa atccactatc cagggtacca tttctctatt tattatataa atatctgagt
     6301 ctgtacttaa agctctcact ctatctaatt tactaccaag aactttagat acttttagat
     6361 gctttagatg ctcccatttt tttacttcac tactccaggt atctcttgca actcttaaag
     6421 gtgctataac taaaactttt gatatatcaa agtaatcata cattaattca cttatagctg
     6481 ttagagcaca gactgtttta cctaatccca tatctaatag taaacctata ttattattat
     6541 ctatagtttt tctaattgtg tattcttggt atggatgtgg tttaaattcc atcccttaac
     6601 ctccttgata aaatcatcta tctcttttaa tgtgcttaca cacttaactt taaatcctaa
     6661 ttctcttaat tctcgcattt tatattgttg aagctttctt agttttttac ctggtgcttt
     6721 aagttctaca aatataacat gtccttctgg taataagaca atcctatctg gcacacctgc
     6781 ctcacctggt gaaataaact tcatagcctt tccacctaac aactcaatct cttttttaag
     6841 tcttttttct atttttgatt ctaacaaaat atcacttcct taaaatttaa gtgtcaacaa
     6901 tgtcaacaga ttttctatat atatgtatat atacatatta ggcatacata tgtatatacg
     6961 tatatgccta atattactta tactacttta tacttaattt ttgttgacat tgttgacatt
     7021 aatacctcaa acattgtaat tactgcatct taggtgtcaa cataagcgtc aacaaagcca
     7081 ttttttttgt tgacattgtt gacactttta aaattttcaa atttactcta tttttaattc
     7141 tgttgacact aacttttacg tacataagct ctctgcctac catatatttt cccaaacctt
     7201 aaatttccat tatatgatat ccaatcatct aatcctttta atatatcatt aatttctctt
     7261 gataagatag gcgtgagttg tttaggttct ccattaaata gctcaaccca tatctccata
     7321 acacacgttt tttctcttaa tattgtccct tcttttaaat caccaaaatc tgaaccatga
     7381 atatattctc tcttttctga aatacttaaa tcataccaat tcttagtaat aggtttattt
     7441 agatactctt cgataattcc agatttagca ttttcttctg agtgagttct ttgttgtctt
     7501 tcagcttctt ttttctcctc atctgataga tatagctgtt cattggcttt atataattct
     7561 actgcttctg cccaaatttg atttctttca ttatcaagtt ggccattaaa tatactcttg
     7621 ttagtcttct ttactcctgc atctattggc caaaaccttc tattcccagt cttatctctt
     7681 aaaaattctt tatcattagt tgttcctata actacacatt gacgtaaaaa tcttgaggtt
     7741 ctcttcccat atgcgaccct atatatatcc tctgatttgc ttagaaaatg cttaactgcc
     7801 tcaatatctg cttttttagt agccatcatt tcacccattt ccagcaacca tactccttgt
     7861 aattgttcat acgcttcttt accttgtaca gtagttaaac tatcagaata ccagtctcca
     7921 ccaagttttt taataaaagt actttttccc attccttgag gaccagataa aaccatcata
     7981 ttatcaaact ttattcctgg attaaatact cttgctactg ctgctactaa aacttttctt
     8041 attatagtcc ttgtatagtg gttatcttct gcacctaaat agtcaattaa aagtgtatcc
     8101 actctcttaa tgccatccca ctttaaagaa tttaaataat cttttatagg atgaaaagta
     8161 ttattttcaa aagcaattat tagagcatca tttacttttg atggtgatga gatattgtaa
     8221 attgtttcta tatgatgtct aagcccagaa tcatcactat cattccaatc atttaattta
     8281 tcatcttttc tccaaggtaa cttacctaaa acgacagctc tatttgaaaa ttcattataa
     8341 gctatttttc ctttcaaata tgggtcattt tctataaaca ttaagatatt gtttgttgtt
     8401 ttcttataac ttcccttatt gtcataatct aacctagtta accattcatc atcttcaaaa
     8461 tctatatcac caaaatcatc cttagcttta tcaaggtttt ctcttcctat agtctttcgt
     8521 actttagtgt cactgcttgc aaattcgctc attcgagtaa atgaaggtaa tctatttaca
     8581 ggcgtttcgg gtttagcatc ttcatcaagt tcaccaaatt tatgtatcct aactaaatca
     8641 aaagcattgc ataaaattcc gcttgctggg tctgtaccat gatgactata tgaaaacttg
     8701 tcatcataaa taactactcc accacttgta ctaccttctg aatatgtata cctggtttca
     8761 tcaataccag gaatatatac ttcatttaag aaagtttcta tagtttcttt tatactatag
     8821 gacctgcaaa atgcacctat aattccagcc ttttcaatag ggtcttgttg tttttttaat
     8881 tgtgtattaa acttttgcct ctccctcgaa ctttctggcc aatagcttac atctgtccag
     8941 tctaaatata aatctagtat ttcatttggg tttaaaaact ctccatcttg aaatttaaat
     9001 atatagtctc catcaattga agtacttgga aaatacataa gtctatgagg ttgatatgta
     9061 gtatcatcaa acatatctat tcctatagta tctgctatca tcctggaaat tgcttgatat
     9121 tcttctggta gtactggtct agttaaaggt attacaagtc tatatctggg attattctca
     9181 gtatgtgagt gcgtagaata cataagacaa gcataatcat ttaataatgt tatatcctcc
     9241 catatatctt tattcgcata atctatatct aaagttataa tacttctatt tgcaatattc
     9301 tctgcttttc gtcttccatt ttttaagctt ccacctacaa atccaccaac atctttaact
     9361 ctatcttttt cagtctttga catcttccta tattctgtat atgtttcttg agttcttaat
     9421 gttttactta atctatttac aagttcggac cataaaatac ttttattttt ccaatgtgtt
     9481 tctaatttat tttttcctat ggccagcatg agttggccat catgtcttac atttatgtgt
     9541 tcaatttcac tggcctttat atccataaat caatcaccta gtctttctta taataatcac
     9601 attcatatcc atctgcttta agaggaagac ctttagccca agatatttct tttcccataa
     9661 tactgttaac ttcttctaaa gaacctcctt ttttatctac atcaattaca agctcatcat
     9721 gtacatgcat tacaatgcta taacctgcat ctgtcacatt aaacatagcc tctcttaagc
     9781 aatctctagc tgtagcttga acaatattct caactaactt aggtccataa gtatctattc
     9841 ttttccattg tttacttgtc tgttccatac cttcatatgt tatcttatcc ccactaaatg
     9901 tagtatgtgg ctctatctta ggccttagat acgataatct tctactactt ggtagttcta
     9961 taaataaaac gcctggatta taaataaatt taatcccatg ttgaatttct actatagtcc
    10021 tatcttttat agctttttta gctgccttat ctacatccca ccaaaactta gttatatttg
    10081 gattagcatt tctccatgca gtaacaatag gttgaagctc ttcttcttta agccccattt
    10141 taatagcccc catagaaatt aaggccccta cacttccgcc atatccattc gataattctg
    10201 ctagctttcc tttttgtcta agttcagaac cttttttaat attttcgatt ggaattttaa
    10261 acatctgact ggcactagct tcatatattt ttccatgaga attaaacaca tccagtctcc
    10321 acttctcacc tgcaagccaa gctataactc tagcttctat tgcactaaaa tctgacacta
    10381 taaatctatg accttcactt ggtataaaag ctgtccttat caattgactt aagacatctg
    10441 gtacactatc atataaaagc tctattaaat caaaatctcc ttcttttaat aggtttctag
    10501 ctaggtctaa atcctctata tgattttgtg gtaaattctg tacttgtact aatcttcctg
    10561 cccatctacc agtcctatta gccccataaa actgtagtag acctcttact ctattgtcat
    10621 tacctttagc taatttcata gcctcatatt tctttataga agttttggac attaattttc
    10681 taagttctaa aattctaact acattttcat catcaacttg ttttaatatt tctggaatac
    10741 tttcttttgt taggcttgta atctcaaagc caactttatc acttaaccat ttttttaatt
    10801 gagctggact atttggatta tttagaccag ttattttaat tgcttctttt gtaagttttt
    10861 cagtatatct tttatcacat tctattgcat tctctattaa ctctgtatcc actttaatac
    10921 cagtatcatt aattctctgg tctaaatacc ataatttaat ttctctttcg gtagtcttat
    10981 acttactaag tttgtttctt atttctcttt caactacaac atcttgttta caatattctt
    11041 taaatttatt ccatttttcc atatcatgta ttggtaaatt tctagttctt cccttgttaa
    11101 cttttgtagc cttacaaggt ttacagaaat attgtattaa tgctttacct tctttcattt
    11161 tctgtttatc ttcattaaac tttaaagcct tagacacact atctaaactt cctggaagtc
    11221 ctagtgttaa cgcctttatc attgtacatg accactcatt tggttttaaa ttaatattta
    11281 aaaatttact tattgatgtt ctttcaaaat tagcattaaa tgctgatttt ataactttat
    11341 tatcatttaa agcttctatt acttcttttg gtaactcttc atcatttact aaatcaataa
    11401 cttttacctc ttcattatca aaagcatagg caaataacag tatctcaaaa ttagcagagt
    11461 ctacatatct gtagactcca acttttttta tatctaaatc actatatgtt tctatatcaa
    11521 ttgataaggt cctcattaac ttaagaagtc ctcttcttca tcttcatatt caaagtcatc
    11581 tgagaaatct gcttctgcac tagctctagc tcctccaagt acttctccat ctgctaactt
    11641 ttgaacattt tgtaagccac aacctattcc tttatttcct gcactattat atgggaaaaa
    11701 gtttatacta actcttccat aacaaccact atatacctct gtattatcta atatttcatt
    11761 taagtcttta tcaactattc ctggcttttg agtactattt gcatttaaaa aatacattcc
    11821 tacatattcc tctgcttcat cagctctttc tgcatctcca tcacgaagag gtgtttttaa
    11881 attacctggt aattttccac cccatttaga agttttacct tgttctttag ctgcatcaat
    11941 agccttcttt attcttccta aagtcacctt atctgatttt ggtattaaaa tacaaactga
    12001 atacttgggc tctgcacctt ctaccattgc tctgctttta aagatattgc aataacttaa
    12061 tcttaccttt cctgttacta cctttgttga ttgtactgaa ttactcataa ttttcttcct
    12121 cctaatattt ttatatttgg tatattgatt ttatatttga agttatttct ccaaatttat
    12181 ctaaatctct aacttgctta ctaaacaatt taattcttcc acaaataact gttgaagaaa
    12241 catttaataa ctctcctata tcagatatct ttttatcttg taaaatccaa tctactattt
    12301 tatcaatatc ttttaaatat gactttcttg caatttttct tatataatca acatcatagt
    12361 taaattgaat ttcataaaat ccatgtatat cttttaatgg tcttttacta tcttcaaata
    12421 cacttacatc taaaccttct atagagtttc tataatgttt ttcgccataa cgcaaagctt
    12481 taaacatttc tattcttatg taagaaacag ccactgtaga aaacttgcct ctgttactat
    12541 cataatttaa tgctgcttta tataacccaa tacatccttc ttgataaaac tcttcatata
    12601 agtatggatg ttgctctaca taagacttaa aaaatctatt tatactaaaa tgtacaagat
    12661 tcaaattatt ttcaactagt ttagttattt cttgattact cataaaatta tatccccttt
    12721 taaatctttt tatagctact aggaattaaa tatttttaat tatcctttgc tcttactgat
    12781 acaaagcata ctggactaac ttttatatgt tctggtatat acactctatc aagatgattg
    12841 tcatttactt cgaggaaagt tgacatcact ttatccttta ttaaactttc tcttttaaaa
    12901 tccatcacta taacttttgc attttcttca gttgagttat attctgtaat atttagtatc
    12961 tttaacatat ctagtaaatt atctttttca taatctaaat ctttttcgat actctttttt
    13021 ctatccttta aatctaaaat tatacttatg ctttcatcta atttatcttt tatacctgca
    13081 tctaatggca tgtaatatcc cccatttata tttcaaaatc ttttttcgct gaatctatac
    13141 tattaatttc tgacctttta tcactttcta ctactaaagt agcttttcca actggtttta
    13201 taattaaatc acttagtagt ttagcaaaac tctttttacc tatagctttc tccatatcac
    13261 taattccttt taaggtcctt ggcttataaa ttttttcttc atcataatct gaatttaata
    13321 aaaccttagc aacttcttgc tcatctacat actttctatt acttctgcct tctacaagct
    13381 tatatcctgg atatttaaca ccatgctttt cagcttgttc tagtgcataa ctttgaacat
    13441 cttttaacca atcttgtata ttcttagcaa aacctaaaat atctgctatc tcatatttat
    13501 taagagcaaa tgtgtcagca aaatcatatt ttctagctag tttaaggtta tcctcagctc
    13561 tttttctgca atcattttta gctctacaaa atccacaatg actgccactt acaaactctc
    13621 cttcaccatt aaaagccatt tgagccttct tcttaacatt atctgcccat gtaagtaatt
    13681 tagtaacttc tatttcttca cttgatatgt tatcaagtct aggttgaatt attgttgttt
    13741 taattaaatc aatatcgtat agcatttcaa actgattata tgcccctaaa ccatatagtc
    13801 taagttgagg attctctata gctgaaactt ctaaaccttt tccatacttt aggtctataa
    13861 cttgaagtat accatcagat attacaacaa cgtctcctgt tccaaatcct tctggaaccc
    13921 actcactaaa atcgagtctt tcttctaaca tcactattac atcatcacat atagctttac
    13981 tatcatttac taattcaact acattctcaa cataagattg tatatagtcc tccatttcag
    14041 agttataata ctcacttttc tgtatctttt taattcttgc attatatgcc tttttactta
    14101 ttttttcata ctctagcatt aatttaactt ctgctaattc atgtgcaact gttccttctt
    14161 ctgcatatat actagttgat ggtggatagt tttcttctaa ttttatacta ggagtgcagt
    14221 gaagccatct atgggctcca ctcgcactaa gtcttgcatg ttgtaatggc atttatgtac
    14281 cccctataaa ctttctaatt tgttcataaa agctgaataa tcttcttctt ttacttcact
    14341 taacttacta gctccaaatt caccaaataa ctccttaagc ttatccttct tacctgcctt
    14401 acttacttgt gctgccttag ttcttacctc ttcttttgtg tatttaactt cactagttgt
    14461 attttcatta ttcttttcta tttcttcctt tgcttctgct atttcttctt taacgtcttc
    14521 ttttacttct acctttttaa tcttttcagc ttttttaact tcaactttct tctcttcttt
    14581 catatctgtt atatttatag gttctatctg cattgctttt cctagattaa gacctcctaa
    14641 agcattcgcc actactaata gtgcatttgt aaattctggt gcttctactt taacttttac
    14701 atttacattc acttcaacca ttttaaatct ctcctttttg tgttatactt tacttgtgtt
    14761 atattttatt ttttattttt ttgtgtgttg gttattttac caacactttt ttatttaata
    14821 ttccaaccga tattttctta ccagttttaa tatctttaaa tactatatct gctataactt
    14881 ttccatcttt tttaagagtt actacattct tattattagt atcaagactc agcaatttca
    14941 tcccccctct ctactgcttt taaaagttca tccaaatttt taccttgatt tctttcaata
    15001 aaatcatcaa cttcatatct tgaaattttt ctaccatcac ctctaactag tgattttatc
    15061 aaacctgtgc ttactaacct acgcataaaa gctgtatcta actttaaaat tccccttgct
    15121 tcttctactg ttattagata atttggataa cctcttttta ttaaaacaac tatatcttta
    15181 ggctccagta cctttacctt ttgttcaata gtttgactct taactctatc tgtctcttgt
    15241 ttacttatct ctatttctat taagcttttt aagctatcac taaattgttt tgttatgctt
    15301 tcagaaatat ccatgtgaaa atgctctccc ctcttttcaa ttatttatct tccaaccaat
    15361 tcgtctaatg taatatctaa ataatcagct aattttatta gagtatctat agttggatta
    15421 gaacttttat ttctcagtaa tttatataaa ccacttacat ctatatccaa ttcttttgcc
    15481 aatttataag gttttatatc tctttttctt agtatttgct ttatattgtc gcctattaac
    15541 attgtattca cctcagttct acatggtata atataaacat ggaataaatt ccaaaatttt
    15601 caaaaggatg gtgtattatg cagttttcaa acaatatatc taaagaatta caagaaagta
    15661 taagaaagac agtctctgat acttctaaca cagagtattt ttactatgct gaatttcaat
    15721 ataaaattat tcttaaatct atagaggaat ttgaaaaaga acttgatgat gaacatgaaa
    15781 ttgcactaaa attgactaac tttggaaaag atgtattaat gattgttgaa gaagtaggtt
    15841 atcataaccc ttgtctaatt cactactacg gcatagtaaa tggagtttat tctgaaattc
    15901 ttcaacacac atcacagata aactttatga taacttcagt aaagaaaact gacccttcaa
    15961 aacctgctag aagaataggt tttatattgt aattatcatt cttattttct gattctttct
    16021 ttagtaattt cactaatttt tcttttttca cttttactca tctcatgtaa atacaaaact
    16081 atacattggt acatataatc tataaaatca ctatttgatt gagctgttat ctgactaaat
    16141 aactgtgctt gcaattgatt ttcaagcatg gttattcttt tctctatttc ttctattttc
    16201 ttattttcca attcaatctc tcctttccaa aatattctgt atttagtttt caaagtacta
    16261 acctaatatt gaagtttgat aactatattc tttttctata tctggtaata tgttatttgt
    16321 tttcaataaa tcatataaaa acaatctacc tttttgagtc cacttagttg tcatttttac
    16381 atcaggcatt ccattacttc tagttatgtc tattgtttct gaatgagtgt atcccttccc
    16441 ttgatgttgt ttatataaaa gccattgtcc actttgttta tattgaatcc ctctttcatg
    16501 aagtattttg ttcatttctt ttccactcat tccataatcc tttgctattt gagttatagt
    16561 gactagacct ttgtttttaa gtatcatatc tgtataatct gcctttggtt ttagttcttt
    16621 tattacttgg tctttcattt ttccttctag ttgtaatttc tcatttactt ctacttgctc
    16681 tataagatgt tgtaatgctt ctttatatgt agttggtagt ttaggttgtt cattttttaa
    16741 tacttgctcc atttcttcaa atttttttgt atatattcct gtaaatgccg taccttttat
    16801 gcctgtcatt ttatttgcta tgaagtcaca acctatcttt gttattgcat agcatggctt
    16861 ctctctttta taatcatcta aataagtatt ttcaataaaa taatcagatg ggtacaaatc
    16921 tgtactcatg tttttattgg cttcttccat ttggtttata taatttctta tatcccttag
    16981 taaaatcttg tgttcttttt caaccattag agctatatct ctactgtcag ttgttagttt
    17041 attattaact tttactattt ttaaatcttt catatttaac tcctctcttt taaacaacat
    17101 cttgttgtat ttgtatttaa aaaaattttt tctattgata ctccaaaaca tttagataat
    17161 tttattgcta ttgctaaact tggtactctt gttccacttt caatcattcc ataataacta
    17221 gtagttatac ctacttttaa tgcaacatct ttttgcttta aattcctttc tgtcctaagt
    17281 tcctttagat tgttcaagct atatcctcct taccaacatt ttgttgttta tacttatata
    17341 atacacaact atatgttgtt tgtaaatagt ttttttattt ttttatttca ttttacaact
    17401 atttgttgta ttatttaata aaggcaactt tattaagagg agtgattata tgagcatttt
    17461 atcagataga ttgaaatttc ttaggaaaga aaaaggtgta atgcaaaaag aaattgccaa
    17521 ctatcttaac ataactacta gtgcatatgg attttatgaa caaggtaaaa gaacacctac
    17581 cccagaaatg ttatctagtt tagctgaata ttttggcact actgttgatt atttaatagg
    17641 taggtatgat aataaagcaa gcaatatttc tagcaaaact tcttgtaata atacattatt
    17701 tcaaaaaaga ctaaaagaac ttagagctga aaaaaatatg actcaagaag atgttgcaaa
    17761 taaattaaac ttaaccaaaa gcgcttatgg ttattatgaa caaggaaaga cagttcctga
    17821 tgcttatatg ttatctagcc ttgctgaaat atttaatgtg actactgatt atttattagg
    17881 taggtctatt gtaaaaaatg atatagacac tgtagctgca catagagtta atcctcacaa
    17941 agatttacca gaggaagctc aagaacaact caatgattat attgaatttt taataaataa
    18001 gtataaaaaa taaattaaaa gtaggtgata aattatatga aatctataat atcagatgtc
    18061 ataaaccgat ttaatgctac accattttta tttgttggtt ctggtttaac tagaagatac
    18121 tataatcttc caaattggga ggatttacta aaagtctttg cagaaaaaat tagtaatgat
    18181 gactttatat atacaagtta taaaaataaa gcaaaatcag agaacccaaa aatgggtata
    18241 aatcctagaa ttgcagaact tattgaaaat gattttaata aaaaatggtt ttcagattct
    18301 tctatacgaa gcttagactc taagtattta gatattgtta aatcaggagt ttctccattt
    18361 aaggcagaaa ttgctatgta tattaaaaat aattctaaaa tagtagataa atatcaagat
    18421 gaagttgaaa aacttagtaa tatctctaaa aaaagtttat caggatttat tacaacaaat
    18481 tatgattgtt ttttggaaac tatcgttgat aattatacaa catatatagg tcaagaaaat
    18541 ttagtttttt catctataca aggtattgca gaaatttata aaattcatgg atgtgtttct
    18601 tctccaaata gtattgtaat aaatgaagct gattatatag actttgacag caaaagtgcc
    18661 tacttggcag ctaagctaat gactatattt gttgagtttc ctatcatatt tattggttac
    18721 tctgttacag atgtaaatat aaaaaaaata cttaatgcta tagttaattg tctatctaat
    18781 gaaaatgcta aaaaactcga ggaacgtttc atattcattg agtatgaaaa aaactttaat
    18841 gatatagaaa tatcaagtca tacaattgct tttgataata aaatgattac tatgactaaa
    18901 attaaactgg aggattttaa tttactctat gaggcattat ctgaaaaaaa atcaaaatta
    18961 ccagttagaa tattaagaat gttcaaacaa gaattttacg atttcacaat tactaacaaa
    19021 cctactgcta aaattcgggt tggtagtgtt gatgatacaa gaataaaaga tgaagattta
    19081 gttttagcag ttggcaaggc aagtgatttt ggtcttaaag gattaaaagg attaagtttt
    19141 gatgaatggt atcgtgatat agtcatgaat gacttagaat tttcatcaga tgaattactt
    19201 gaatatgctt atccatcttt aattaagcaa tataataaat taccattaaa taaacatctt
    19261 tttaatagca cacttgaatt tccagattat aggacaattg cactagaaag tgactttgaa
    19321 aatattatta gcaatagtat taaaaaaaat agaaataata cttacataaa aaatagaagt
    19381 gtttatggta tttggaatga tgaacatcaa tcatttgaaa aagctacaag acttattgct
    19441 tttttagaag aaaaagaaat tgacactgaa cagttagaaa atctacttaa gaaaatattt
    19501 gaggaaaatc ctaatatttt agagtctgca aaaacaagcg aaaaaacaaa tttaagaagg
    19561 cttattagaa tatatgacta catgaaatac tctaatatgc aaaagagtct tggttatagt
    19621 gagtaaaact ctacccaccg ctccaaaact cctttaatcg ctttaagcac aaatacaaat
    19681 ttagtgctaa ctatactaac acacatttgt aagtgcttac atatattata tgtaattata
    19741 ttataatcat atactataat ccatattttg tctacagcta aaattatcca atcttttttg
    19801 gataaaaaat gaatatttag agcagttcac acctgctctt tatatataac aaaaatcaaa
    19861 aatacattct ttttataggg ggatttcaat gaacaaacta gacgcacttt tagacttagc
    19921 aaataatgaa gagatagaaa tttactacac tgacaaaata gcagatgaca taaaaggatt
    19981 gtatataaac agacaaggac taaagattat atcattactt aattcattaa aacaaaacaa
    20041 tgctaaacta atagaaatct tagcagaaga attaggacat cattttacca gtgttgggaa
    20101 ctatgtatct tcaaaaaaca gttacaaaaa taaaatcttg atagacaaaa ctgaaaacaa
    20161 agcattaaaa tgggcatgtg aatttcttat aacagaagaa gaaataatac atgttattaa
    20221 ttcacacgct acaagtgtat acgaaatagc tgaagaatta caagttagca tcaacttctt
    20281 actaaaaaga ttagaatttc tatcaaaaaa gaaaagcatg ttggacttag gaaataatag
    20341 atttttagta ttaactaatt tgccaaattt ctacatatat gaggatattt tttaaactca
    20401 tttattctac ttttatagat tttttactta ataaatatat atttcaatat tattataata
    20461 aactacacat aaaagctaaa aaattgtaag aatattaaga aaataattaa gtaaaaacca
    20521 gataaacaaa attaagataa tattgtacat aacaaaagta tataaagagc agttaatctg
    20581 ctcttttata taaacaccaa acaaacatac attctaaaag ggagggatac tattatgaaa
    20641 ggtggagtaa gaaaaagaag taacaaatgg tattactact ttgacctagg catagtagaa
    20701 ggaaaaagaa aaaaagtaga aagagttgga ggcaatacta aaaaagaagc cgaaaaagcc
    20761 ttaagagaag cactaaatga atatgaaaat tctggcatag tatttgaaga aagcaatatc
    20821 agtttatcag actacttaga cttttggtac aaagaatatg tcttacttaa ctgtaaatac
    20881 aacactcaag aaagctaccg aataaacata gaaaaacata taaagccaaa gctaggagct
    20941 tacaaagtaa aagctttaac tcctgcaata ctacaaaact tcataaataa aaagtacaaa
    21001 gaggattact ctcaaaatac attacaagta ttaaaagcca tattacatag gtcattaaaa
    21061 tcagcagtcc atccttacaa acacatacga gaaaacccta tgcaatatgt aagcatacca
    21121 aaaactaaat ctaaaacaga aactaataaa gttaaaacta ttacagtaga agaatttaat
    21181 caaatactaa atatatttcc tcaagattca tttcaacgta tagttttact aattggattt
    21241 catactggta tgcgaagagg tgaaattatt gcactaaaat gggataatat agaccttaat
    21301 aataaaacta tcacagtaag acatactctg attaaaaagc caaatggaat gtttgaatta
    21361 gggcaaccaa aaacagaaag ctcttgcaga actatattta caggtgacac tttaataaag
    21421 gcattaaaag aacataaatt atatcaaaag aaaatgaaat taaaatatgg agaattttac
    21481 tttgatagtg actgggtatg taccaaagaa aatggtcaac aagtgaatac tcacacttta
    21541 gacactatag taagacaaat tcgagtagct ttaaacaatg acttccattt tcattcttta
    21601 agacatgcac atgctactct attattagaa aatggtgcta acattaaaga catacaaaac
    21661 cgtttgggtc atagccaact atcaactaca atggatacct attcacatgt aactgataaa
    21721 atgaaaaatg aaactgtaga tatatttgaa aaaattacaa attagagttt gccacccaaa
    21781 aatataatac ggtggcaaat gggtggcaaa atctaattta tctattttaa aagctaaaat
    21841 tatcaaattt atatagtcag ctatacgcat gtaatttcaa ggctttagag tatataacaa
    21901 ccataactaa tataaggtat taataataaa tctaatgaat aaaacttaat atttacttaa
    21961 gttgtaatac tattctaaat tgtataatta aaatttaata agttcttaat tatatagtca
    22021 ttgatttatt tgcaaaaata aaaaatgcca atctatctct aaacaacaaa aactgaatac
    22081 taataaaaat ttaataaaaa attttatcaa tattcagtat aagttttagt tacatatcaa
    22141 attttccaat gcatcattag gtgctacaat aggataaaca tcataatcct tatctatatc
    22201 acattgcaaa aatacaggtt tattaaaatc tatcatatct agcgcctttc ctaattcttc
    22261 catactggat accttatatc catcaatgtt atatgcattt acgagtttaa cataatccac
    22321 attttcatta atgtcagttt gagagtatct ttgattggaa aataactttt gccattgcct
    22381 taccattcct aatgttctat tattgagtag taaaataagc ataggtacat tataatttgc
    22441 aactgtagct agttcattac agttcattct aaaacttcca tcaccagtga ctaaaaccac
    22501 atttttatct acattaccaa ctttagttcc aatagctgca cctaaaccaa atcccatagt
    22561 tcctaaccct gctgatgtta taaaactttt attaccttta aaattccagt acttagcagc
    22621 ccacatttga tgttgaccga catcagttac taccacagtg ggtttcttta atgtttcata
    22681 tttttcattt atttttttaa gtatattttg tggatgaaat tcatatgttt gtacaccctc
    22741 actctttcta aatctcttta tctcttcttt ccaattgcta ttattcttac tttcaaccct
    22801 ttcaattaat aaactaagga ctagctttac atctccaacc aaagatacat tagattctat
    22861 atttttactt atttcagatg ggtctatgtc tatgtgtatt atttttgcat ttttagcaaa
    22921 ttcagaactt ttacttatca ctctatcact aaatctcgca cctatcgcta ttactaaatc
    22981 tgaattagaa agtgctaaat tactttctct acttccatgc attccaacca ttccaagaga
    23041 taattcattc tttctatcta tatttccaag ccccataagt gtatttagta cgggtgtatc
    23101 tatcttagtt gcaaattttt caagaatttc ctcactgtct gaagatttaa cacctccacc
    23161 tgcatatatt ataggctttt tagattcttt tatgatatct attgcttcat ttaaaagctt
    23221 aatattagtt tcatcatcta gatcaaaatc actcttataa tccatataat catctatttg
    23281 acataaatca tagtcctcac cactaaaatc catttctgct aaaaataaat cttttggaac
    23341 atctacaagt acaggtcctt ttctacctga atttgcaact ctaaaagctt cttttattgt
    23401 aggaactaat tctttagtgt ttctaactaa ataattatgt tttgtcatag ataatgttgc
    23461 tccagttatg tcaatttctt gaaaagaatc ttttcctaat aaactagttg gcacctgccc
    23521 tgaaattaca accataggtg aagaatccat aaaagctgtt gctattccag ttatggcatt
    23581 tgttgctcct ggaccagatg ttgtaaaaca aactcctact gtattagtac ttcttgcgta
    23641 cccatctgca gcatgaacta aaccttgttc atgggatgtc cttatatgtt taaaatcatc
    23701 tgaataatcg tatagagcat cataaagagg tatcacggct ccaccaggat atccaaatat
    23761 tgtgtcaatt ccctctttct ttaaacattc taaaattacc tttgctccat tcatacgcac
    23821 tactatacac cactctttcc ctcaaactaa tccatttaat atatatttat tttaagaatg
    23881 acatcatact tcttagattt ctaccaactt cggcaatttc tgtattatat tcttcttctc
    23941 tagtttttag gaagttttca cgtccttctt cattttcttt aatccacgct tttgcaaatt
    24001 ttccattttg tatatcctct aaaacatttt gcataccttg ttttgcaaca tcagttatta
    24061 cccttttacc agatacataa tctccatatt ctgctgtgtc acttatacta tatctcattc
    24121 tttcaaatcc accttcatat ataaggtcga ctattagttt catttcatgt aaacattcaa
    24181 aatatgctac ttctttttga taacctgcat ctacaagcgt tttatatccc aattttataa
    24241 gttcacttat tccaccacaa agtactgatt gttctccaaa taaatctgtt tctgtttctt
    24301 ctttaaaagt agtttctaaa acacctgctc ttgttgctcc tatccctttt gcatatgcta
    24361 aaactgtctc tgtagctttt ttagtgtagt cttgatatac tgcaaatagt gcaggaactc
    24421 cacttccctt tgtaaataca tttctaacta agtgaccagg tcctttagga gctaccatta
    24481 caacatcaac aaattctggt ggttgtactt gattataatg tatattgaat ccatgagcaa
    24541 aagctaatgt ctggccttct tttaagttat cctttacact ctcttcataa actttctttt
    24601 gtttttcatc aggcattaac atcattgtca aatcactttc ttttgtagct tctgctacac
    24661 tcttaacttc aaatccatct tcttttgctt tttgtgctga tttacttccg tcataaagac
    24721 ctatcactac atgtacaccg ttatctctaa gattttgtgc atgtgcatgt ccttgacttc
    24781 cataacctaa aactgcaacc tttttatttt ttaaaacttc taaatccaca tctttttcat
    24841 aatacattct tgccattttc atttcctccc aatacttttt cttattttta tgatatttat
    24901 ttttattaga aattcatact cattatcatt gttttaatat tattcaacag atttaaattc
    24961 taaatttata taatcttaca attataattt tagaattttc agtttttatt agctacaaca
    25021 aactaataaa tttattaact gtgataaact aataaattca atgacactta tattttaatc
    25081 taagattttt cttatttaat atttatcttt agttttttat tttaaaataa gcatatttat
    25141 ctttagcttt ataaaatata tttaattaat gtctcattct ttcaacagtt atctctctta
    25201 tatcatgtag ttttttcata tggtttaaga cagaatctaa ggatgtcttt tcttcattta
    25261 ctattaattt tatacctgaa ttattcatat tatctacagt catacttatt gattgtatat
    25321 caacctctct tcttcttaga gtcatagcca ctcttaaaaa agaatctatt ccttggttta
    25381 atttagcata tacagtattt tccataaaaa tccctcctta tttttattat ttttttatta
    25441 attttatatg gattaaattt atttatatcg cttaaaataa aaaaactttc atctttataa
    25501 gcaatataaa tattacttat aaggacgaaa gttatattcc gcggtaccac cttatttgta
    25561 gatataaaaa atacatctac ccctcttttt tcaggtcggg taattaatta ccttaacctt
    25621 agtcatgtaa cgctgacata cgtttgtact tactaaaaat attcagtaca aaagctccag
    25681 agtgatcatt atactactgt taacaccaat tctcagcaac attggttctc tgtggataac
    25741 actattagta ttgttgtctc tgtcattgct tttaattttt taattttata ttttaattct
    25801 gttttgttgt taatatagat tatattccct atcatttaca atgtcaatga ctttttttaa
    25861 aattttcaga ttatttattt ttttcacaat aatacttctt ttatacttta cgcctatatt
    25921 ttggaataat cgttttcaga ttattatttt atatccattt atttttagcc aattttcttt
    25981 atctttataa tcgggcataa tggattttac taaatcatag aattttttag agtgattcat
    26041 atgtactaaa tggctaaact catgtattat tacataatca attacatcta atcttgccat
    26101 agatattttt gagtttatat aaatattttt ttgagctgta caacttcccc atctcttatt
    26161 ttgttctttt accttaacac tcgctggaac taattgcttc attatttcac aatttaattt
    26221 aagaaaatct attctctcta atactattct ttcactttcc atcttatacc aagattttaa
    26281 agatttttta ataaaatctg tacttgtatc atttgtctga acaactattt gatattcgtt
    26341 aatagttatt ctacagttac tttttttgac agaatctctc aacagttctt ttataactaa
    26401 atggtactct tcacctaaat aaagaaattt ttcgccagtt ataaacattc tttgtttata
    26461 tacttcctct cttcctttac atttttctga ctttttaagt atccagtctc ctttttcaat
    26521 aattatatct tttattattt ttttggatat tttaggagga ctcattacag ttattttgcc
    26581 aaatggatct atttttatag aaatattttt tctattttta tatactaaaa ctgcttttat
    26641 ttcaatccct ctaaaattaa cctttaaagt atcaatatct ttactccctg tgttcattag
    26701 tttatttttc cttattagct ctacaattta taagttcagc agctatacat acagctattt
    26761 cagctggtgt ttgagcattc aattttatgc ctattggtgc atgtactttt gataattctt
    26821 ccttcttaaa accttttttc atgagttttt cgtaagtcaa tccaattttt gttctacttc
    26881 caatcatacc tatataacca gcctctcttt taagagccca ttctaaagca cttgaatcat
    26941 atagatgacc tcttgttaaa ataattatat aactattttc gtcagttgga aaatctggta
    27001 tgttttcaaa agaatctaaa actactactt ctgattctgg aaacctttca tgattagcaa
    27061 attcttctct atcatctatt actactgttc taaattctaa aagagatagt atcacagcaa
    27121 catctcttga aatatgacct gcaccaaata tatatgcttt acttgtattt tcctttagtc
    27181 tcttattaaa tttttctata ttacttttta atttactatc tataaaatct atttgtattt
    27241 ctccagtacc tccacatatc attcctagtt ttgcaacatc agatttgttt agagtaaatt
    27301 tatacaacat tgattctctg ttttttataa cattaattgc atgttttata cattctgctt
    27361 ctacactacc tcctcctact gagccaaata tagtagaatc atttttcaca atcatcattg
    27421 ctccagtttc acgtggtgtt gaacctgaat gagacgttat tgtagcaaat gcgaaatcct
    27481 cattattttc tattaatctt attgcttgtt catatatatt catataattc tccttaccag
    27541 tctatctaaa aatcttctat atatctatct tagtcttttt attactttat ttggtatcac
    27601 aaattctact gctcccatat aaccaggagc tacttcatat tcatcaaaac taatcacaag
    27661 atttccattt ttattaaaat aaaaatcttg atacttattt atagattcaa aatctttcac
    27721 tggcgagtct ttatcttggt ctataaaata agattttgta tcatctttct taatttgatt
    27781 tctcatctgc tcttttatat tatcacttat agctctaata tatctactat ctttaaaaaa
    27841 tattggtaaa tttaatagta ttcctctatt tttatcaaca gtgtaatgct tcttagttat
    27901 agatgaggaa gcttctatct cttcttttgt catttctatt gataaaaagt ccttagaatt
    27961 atttttgaca ttatggatta catttaggta tctatgaccc ttattatctt cctttttcat
    28021 tttattttga aattcactgt aaattttttt cccatcttgc aaaaactcat tgtttaattt
    28081 attctctaaa gttttatctt ttaaaccttt tattttagga atactcacat tcacttcata
    28141 attgtcttgt ttaaagttga tactcttagc atttaacacc tttatcgtat cactatttga
    28201 agtttcatta aaggaactac ctgcaaatat agggtttgta tctactaatg aagctaataa
    28261 gcatattgaa aatatagcaa ttttaaatat agatttttta agttttttat atgtagtcat
    28321 attgtcctcc ttaaaagatt tatatataaa ctactattca atttacaaat cttttattac
    28381 tttatatggt attacaaact caactgcgcc catataacca ggtgctactt catattcatc
    28441 aaaacaaatt acaaggtctt catttttatt aaaataaaaa tcctgatact tattaattgt
    28501 tttaaaatct tcaacaggca agtctttttt ttgatctata aaataggatt ttgtactatc
    28561 ctttttcatt tgttctctca tttgttcttt tatattatca cttataactt ttatatactt
    28621 atcatcttta aaaagcattg gcaaagtcaa aactatttgt ctttttttat caatagtata
    28681 atgtacctta ctcacatatg atgaagcctc tatctcattt tttgtcatct ctattgatag
    28741 aaatttttta gaattatttt ttacactata actcaaactt aaagatttat atcctttatt
    28801 ctttgatgat tgtatcttct ccattctctc ttggaattga tgataaagcc tttttccatc
    28861 ttccataaaa ctactgttta atctttgttc taagttttta tcttttaaac ctcgtatttt
    28921 aggaatatca atctcagctt cataattttc actttttaaa ctataatttt ttatagttat
    28981 tacttctact attgctccaa ttacaggtat cttagaaaaa ttgtctgcaa atacaggact
    29041 tatatttact aatactgtaa atatacatgc aaaggctatt gcaactttaa atcttaatct
    29101 atttactttt ttatttttct ctcgatatat gttttctatt ttttcatgat ttattatagt
    29161 atctaatttc ttgggtattg gaattttatg atactcatct tttaacttat ttaacctttc
    29221 tttgcttgtc attcttttta tccctactct tcaattttta attttagttt ttttaaagca
    29281 gtatataacc tagttttaac agtattcaca ttttcatcca atattattgc tacttcttca
    29341 atctttaaat cttcaaaaaa tcttaatatt ataatagatt tgtattcaat aggtaattct
    29401 tccaaagctc ttcttaaatc caaatcagta tatttatcat actctccagt ctcatctata
    29461 aggtcaatat ctgtcatatt attatattta cggttttttc taataaaatc tatactcgtt
    29521 ctaactaaaa ttttataaaa ccatgtcttt atataattta cttctttaat attttccact
    29581 gagtatagtg ccttatacat ggcttcttga accacatcta aggcatcttc ttcattcttc
    29641 gtataactat atgctattct ataaaaagag tctttattat cgataatgta tttctcaact
    29701 ttcgttctct ttaaactctt aaacattcct gtaaaatctc cctccgatta caggctagga
    29761 ttgagctgtt agaaattcta tctgtctatt ttagcatctt cattcagttt ttttatatgc
    29821 tcaatatact tttcatttaa aatagattct cttattaaac tctgacactc attaaaactt
    29881 ttttgtcttt ctcttttatc agtaactttg actatatgat atccataact agtttcaaac
    29941 acatttgaaa cttcattttt ctttaatttg aacacttcct ttgtaaactc agcattttta
    30001 tcatcctttg taaaatatcc tagctgtcct ccattttttc cagttgcttt atcatccgaa
    30061 tattttttag ccaaactttc aaaactttct ccatttttga ttttagtcaa tatattatct
    30121 gccttcttct tcaaagcttc ttttttatct ttgcttactt cttttttatt tttatctaaa
    30181 gtcgatataa gtatctgaga agcacttact tcttctacat taaaatcttt tttatgactt
    30241 gtatagtagg cttccatatc attatcactt atgtttattc tatcttcaaa ggccttttta
    30301 tttttatcta ttgctaaatc tctagatatt tcttgtttta aataatcttt atcaacacct
    30361 gctttgtcca ttttttcctt atagcttgta ttcaaattca tcttatcttc tagttcttga
    30421 tattttgagt ctacttcact tttacttgga gttagtcctt tatccttagc ctcttgatat
    30481 aaaagttcat tatccaccat aaacgatatt ataacatttt ctaatttatt tctacctttt
    30541 ttatccaaag ttttttctaa ttcatctaaa gactgcccat ttatataatt attagtagca
    30601 gataaaactg cttttgtttt tttgtactgt tcttttgtga tttccacatc atttactttt
    30661 gctaaacttt tattacaacc aaccatcatc aaagacaata ttccgaccat tcctatataa
    30721 agttttttat tcaatatacc tacacctcac tcatttttat tctcaatttc taaaaatatc
    30781 aatcaatatt cttatatttt aactactcta aattacgtaa ccaataattt tttggtattt
    30841 ggttttgatc tactggcgca aattcataac cttcatctga taagaatttt aaaactgaat
    30901 ctaaagcttc tgcactctgt tttttctcat gcattaaaac tacaagttgg tctttatctt
    30961 caccttttct attcttaata tgatttttta cattttgtac aatttgagaa gaattagatc
    31021 tccagtcttc tgtatctaag tcccagtccc aaattttata tccagcatca actaaagcct
    31081 gataagaggc ttgtggtgta tatggtttgc ttccataagg tattcttatt acttttgaat
    31141 gtttaccagt tatcttataa aaagtttgat catttgtatc aaattcctct tttgctgatg
    31201 tactagtaac ataaagcttg tgaatatcat gtgatacact atgaaaacct gctgtgttcc
    31261 catttttaat tatattttta acttgctgtg gatattcctt catattccca tctatcataa
    31321 aaaaagttgc tttaacattg tatttattta aagtttttat catctggtca gtaaatttag
    31381 atggaccatc atctatagtt atgtatgcta tttttccaga acttttactt ggccctttac
    31441 taacttctat tgatgtatta gatactatat ctgtaatagt agaacttgac agttttgcat
    31501 ttctactaat ttcattttcc ttcatattta aagagctgac gcgaacacca actactataa
    31561 aagccagaca cactacaata gcacttaaaa gcaaatacaa cttctttcta tttatttttt
    31621 tctttttatt tctcatcgtt cgacctccaa tattactata tttttacatt tatatttttg
    31681 tagtatttta tcccaaaaat ttacactaca tatatataga cgttgaactt cgacaaaaag
    31741 ttttgcttaa tttttaaaat ttgttatttc ggtgattatt atgctgtata atttctgtat
    31801 aattgtatag aat
//
LOCUS       DAYATN010000031        31726 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.031, whole genome shotgun sequence.
ACCESSION   DAYATN010000031 DAYATN010000000
VERSION     DAYATN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.031"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            56..373
                     /gene="flgB"
                     /locus_tag="ACGI8A_001412"
     CDS             56..373
                     /gene="flgB"
                     /locus_tag="ACGI8A_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFH0179415.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            381..785
                     /gene="flgC"
                     /locus_tag="ACGI8A_001413"
     CDS             381..785
                     /gene="flgC"
                     /locus_tag="ACGI8A_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFH0179416.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            795..1112
                     /gene="fliE"
                     /locus_tag="ACGI8A_001414"
     CDS             795..1112
                     /gene="fliE"
                     /locus_tag="ACGI8A_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFH0179417.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1137..2687
                     /gene="fliF"
                     /locus_tag="ACGI8A_001415"
     CDS             1137..2687
                     /gene="fliF"
                     /locus_tag="ACGI8A_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFH0179418.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2700..3770
                     /gene="fliG"
                     /locus_tag="ACGI8A_001416"
     CDS             2700..3770
                     /gene="fliG"
                     /locus_tag="ACGI8A_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFH0179419.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3754..4494
                     /locus_tag="ACGI8A_001417"
     CDS             3754..4494
                     /locus_tag="ACGI8A_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HFH0179420.1"
                     /translation="MTLLNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFR
                     DEVIKEAIESAEIRKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYT
                     QKAKDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESM
                     SDLLKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGN
                     AIIQKENGRIIVGIDCAMEKLKEELLGE"
     gene            4498..5817
                     /gene="fliI"
                     /locus_tag="ACGI8A_001418"
     CDS             4498..5817
                     /gene="fliI"
                     /locus_tag="ACGI8A_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFH0179421.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            5849..6295
                     /gene="fliJ"
                     /locus_tag="ACGI8A_001419"
     CDS             5849..6295
                     /gene="fliJ"
                     /locus_tag="ACGI8A_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFH0179422.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDNVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6323..7528
                     /locus_tag="ACGI8A_001420"
     CDS             6323..7528
                     /locus_tag="ACGI8A_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFH0179423.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNNKFEDNLYKTTDHKNV
                     KDRRETSKQNVSSDRPGNSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNLELNGANTNNPNDIE
                     NLDIMKLRELLENNASEDIQVISKTTENDTKSIDLYSFDSNRMDTLNSNKQKDDSSDI
                     LEKLAGLNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSNQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7546..8154
                     /locus_tag="ACGI8A_001421"
     CDS             7546..8154
                     /locus_tag="ACGI8A_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFH0179424.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEDEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVDVKYEKLLKIGEVEANSGEENDSSEQESPEEV"
     gene            8181..9164
                     /locus_tag="ACGI8A_001422"
     CDS             8181..9164
                     /locus_tag="ACGI8A_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HFH0179425.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9177..9380
                     /locus_tag="ACGI8A_001423"
     CDS             9177..9380
                     /locus_tag="ACGI8A_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HFH0179426.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9380..10198
                     /locus_tag="ACGI8A_001424"
     CDS             9380..10198
                     /locus_tag="ACGI8A_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HFH0179427.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEINEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10188..10883
                     /locus_tag="ACGI8A_001425"
     CDS             10188..10883
                     /locus_tag="ACGI8A_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFH0179428.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKEDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10895..11338
                     /gene="fliL"
                     /locus_tag="ACGI8A_001426"
     CDS             10895..11338
                     /gene="fliL"
                     /locus_tag="ACGI8A_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFH0179429.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11353..11727
                     /locus_tag="ACGI8A_001427"
     CDS             11353..11727
                     /locus_tag="ACGI8A_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HFH0179430.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11728..12393
                     /gene="fliP"
                     /locus_tag="ACGI8A_001428"
     CDS             11728..12393
                     /gene="fliP"
                     /locus_tag="ACGI8A_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFH0179431.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12406..12675
                     /gene="fliQ"
                     /locus_tag="ACGI8A_001429"
     CDS             12406..12675
                     /gene="fliQ"
                     /locus_tag="ACGI8A_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFH0179432.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12687..14501
                     /locus_tag="ACGI8A_001430"
     CDS             12687..14501
                     /locus_tag="ACGI8A_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HFH0179433.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLINNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPIAMTLLAFTLLVPTLFSYVVNTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            14510..16585
                     /gene="flhA"
                     /locus_tag="ACGI8A_001431"
     CDS             14510..16585
                     /gene="flhA"
                     /locus_tag="ACGI8A_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFH0179434.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16560..17576
                     /gene="flhF"
                     /locus_tag="ACGI8A_001432"
     CDS             16560..17576
                     /gene="flhF"
                     /locus_tag="ACGI8A_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HFH0179435.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17573..18451
                     /locus_tag="ACGI8A_001433"
     CDS             17573..18451
                     /locus_tag="ACGI8A_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HFH0179436.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVGVCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18465..19166
                     /locus_tag="ACGI8A_001434"
     CDS             18465..19166
                     /locus_tag="ACGI8A_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0179437.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDTEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19182..19604
                     /locus_tag="ACGI8A_001435"
     CDS             19182..19604
                     /locus_tag="ACGI8A_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HFH0179438.1"
                     /translation="MIQIILIVVSILIIVTIVINLQKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            19589..19774
                     /locus_tag="ACGI8A_001436"
     CDS             19589..19774
                     /locus_tag="ACGI8A_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HFH0179439.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19815..20588
                     /locus_tag="ACGI8A_001437"
     CDS             19815..20588
                     /locus_tag="ACGI8A_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFH0179440.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20609..21370
                     /locus_tag="ACGI8A_001438"
     CDS             20609..21370
                     /locus_tag="ACGI8A_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFH0179441.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21393..22274
                     /locus_tag="ACGI8A_001439"
     CDS             21393..22274
                     /locus_tag="ACGI8A_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFH0179442.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNECIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22277..22651
                     /locus_tag="ACGI8A_001440"
     CDS             22277..22651
                     /locus_tag="ACGI8A_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFH0179443.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22683..24086
                     /locus_tag="ACGI8A_001441"
     CDS             22683..24086
                     /locus_tag="ACGI8A_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFH0179444.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYNSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24202..26139
                     /gene="htpG"
                     /locus_tag="ACGI8A_001442"
     CDS             24202..26139
                     /gene="htpG"
                     /locus_tag="ACGI8A_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFH0179445.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            26527..27657
                     /locus_tag="ACGI8A_001443"
     CDS             26527..27657
                     /locus_tag="ACGI8A_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFH0179446.1"
                     /translation="MNFNYNLPVNILFGSGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            27884..28906
                     /gene="splB"
                     /locus_tag="ACGI8A_001444"
     CDS             27884..28906
                     /gene="splB"
                     /locus_tag="ACGI8A_001444"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006290 - pyrimidine dimer repair [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HFH0179447.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29088..29999
                     /locus_tag="ACGI8A_001445"
     CDS             29088..29999
                     /locus_tag="ACGI8A_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFH0179448.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30014..30430
                     /locus_tag="ACGI8A_001446"
     CDS             30014..30430
                     /locus_tag="ACGI8A_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HFH0179449.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(30530..30922)
                     /locus_tag="ACGI8A_001447"
     CDS             complement(30530..30922)
                     /locus_tag="ACGI8A_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0179450.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31063..31458
                     /locus_tag="ACGI8A_001448"
     CDS             31063..31458
                     /locus_tag="ACGI8A_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFH0179451.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        13021 a         3366 c         5828 g         9511 t
ORIGIN      
        1 catttttttt attaataaac aaaatagata aaatatattt tgaggagggg tgagtgtgaa
       61 tacatatgac attataaaaa tgggattaga tgcaactaat ctaagaagtc agacaatagc
      121 aaataatata gcaaatataa atactgaagg atataaaaga cattatataa cttttgaaga
      181 aacactaaat gatgctatgc ctaaaataga agaaaaaaca gacaattcta aaggtttaag
      241 agtagatggt aataatgtag acatagaaga agaaaaagta aatcaagcaa tggcaacatt
      301 acaatataat gctttgataa gttttgcaaa tggaaaaata gcaatgacca aatcaataat
      361 aagtgggagg taattagaag atgagtgtgt tcagtggaat gagaatatct gctagtggat
      421 taacagcaga aagtttgaaa atggatatca tatcatccaa tgtagctaat ataaatgcta
      481 caagaggtcc aaatggagaa gcatatagga gaaaagttgt ttcatttgaa gaaaactatg
      541 ataataaatt aggtatgcta ggagtaaaag ttacatctgt tgaagatgat aaatcacctc
      601 ttaaagaagt atatgaccca tctcatccag atgcaaagcc aaatggatat gtagaatatc
      661 caaatgtaaa tatattggta gaaatgacag atttaataac agcttcacga gcatatgatt
      721 ctaacgtaga tacattgaat gctcagaagt ctatgatatc aaaagctttg gaaataggaa
      781 agtagggata aattatgcca attacacaaa ttaacaattt aatgcaaggt gcaaagttag
      841 ataatttgat tggagaagca aagccattaa ctcttaataa taaagaagaa aaaaagaatt
      901 ttgaagatgt aataatagat actatagata aagttaattt tcaacaagta acagcagatg
      961 atatgaaaga aaaatttata aaaggcgaag atgtttctat gcatgaagtt atgttaatag
     1021 gtcaagaagc acaactatct atgcaatatt taatagaagt tagaaataag attcatgatg
     1081 catatcaaga aatgaataag gtgcaaattt agccttttga acaggagtaa taatcaatga
     1141 taaccagttt taaaaataag attaaggagc aagttgctaa atctaggaca aagataagtt
     1201 ctttaaaaaa gggaactaaa atagcattag gtataggggt tattgctata tgtttagcgc
     1261 tagtatttac agcaatatct gcgagcaaaa ataagtatgc agtcttattt tcagaactag
     1321 atgctcaaga tgctgcagat atatcagcag agctagagtc taaaaagata gatactaagg
     1381 taaaaggaag tacgatttat gtacctagaa cagaagttga taagttaagg ctggagttag
     1441 cgccaagtct tacaggtggt tctaaaggat ttgcactaat ggatgatagt tcatcaatag
     1501 ggcttactga tgaagaattt cagattaaga aacaaagaat gatacaaggt gaaatagaaa
     1561 aaacaataaa gagttttcca caagtggaag acactagagt acatataaca tttggagagt
     1621 catcagtatt tgagaaggag gctgttccag ggagtgcagc agtttatttg atgctaaaac
     1681 cagggacgac tttagaaaaa aaccaaataa agtctgttat ggcacttata tcaggaagcg
     1741 ttacaaatat accagagaaa aatgttcagg ttattgataa caaaatgaat ttattatctg
     1801 aaggtatttt ggaggatgaa aatgttgatg aaaataatca accaataaat agtggcatta
     1861 gtgttgataa aaataaggca tctgaacaag cattgaataa acaactagaa aagtctattt
     1921 tagaaatgct agaaccgata tttggtaagg gaaaggtgaa agctacagta aatgctgatt
     1981 taagttttga tacagctgaa aaaactgaga taaaaattga ccctgataaa gttgcaataa
     2041 aggagacaag gtcgaaaaat tcatctaaat ctgctgcaaa taatgttcaa ggtgtagatg
     2101 ctaacatgaa taaccaagga aataacaatg gtactaatac agaagatagt ttagatgaga
     2161 gttttgagta tgaaacaggt aagattgaaa ctcacactat agttgcgcct ggagaactta
     2221 atagattgac agcttctgtt gcaatagatg gaaaaatagc taaaggtgtt caagctgatg
     2281 tagaggatat tgttaataat gctataggta tggatggagc gaggggagat actcttagcg
     2341 ttgtttcaat ggtatttgac ccagaaggta agaaagaggc taaacaagag ctggaagaga
     2401 ttaaaaaaga aggatttaaa aagaacataa taaaagcagt tattggagta gtagcagtaa
     2461 tagcagttgg agctattgga tacttaatat ataagaaaag aagtgaacga gaagatatgg
     2521 attatgaaga tgaagattat atttctaaat ctgattctgt tgataggaag gataatcctc
     2581 tattaggaga tgacccagaa ctgacattag aggaagctgt taaaatgtat gcagaagaaa
     2641 agccagacca agtaacagag ataataaaga cttggttaaa tgagtaaggg ggatagaaag
     2701 tgtccacgga aattacaaat gttaatgcgt ttgacttaag tgatataaat gaaagtgata
     2761 agaatgagaa ggtagaaggt gagggtttaa aaaaagctgc tgtattatta atgacattag
     2821 gcccaaatgt gtctagtgat ataataaaaa gcttgcctga taaacaagtt caaaaaatag
     2881 gtttggaaat tgcaaatatt tcttcaatag cttcaaaaga aagaagagaa atcttaaaag
     2941 agtttgtaga aataaatagg gcaaaagatt atgttataga aggtgggctt gaatatgcaa
     3001 gagagttatt aagtaatgct ttaggaaatg ttaaagcaaa caaattactt gaaggtatat
     3061 caataaatac aagtacaaag ccttttaaac atattataaa actagatgta aagaagatat
     3121 tagaaatact taggaatgaa agtgcacaaa ctatagccgt tttacttgca catatggaac
     3181 cagaaaatgg agcagaagtt ttatcatact tagattttga tttacaaaat gaagtagcta
     3241 tgagaataag ctcaatgggt tcaatatctc cagaagtaat aaaggtaatt gaaagttcat
     3301 tagaagataa attacattca ataagtggtg atgattttaa tgattatgga ggggtgtcta
     3361 ccttagttca aatattgatt aattctgata gaaaaacaga aaagaatatt ttgggaacta
     3421 ttgaagaaca aaacccggat ttagcagctc aaattaaatc tcatatattt gtctttgaag
     3481 atattgtcaa gttagatgat ataagcatac aaaaagtgtt aaaagaagtg actatgaaag
     3541 atttagtata tgccttaaaa ggtgtagatg atgagatttc agatgctata tatagaaatc
     3601 aatcttctag agcagcagat gcacttaaag aagaaatgga aatggttggt gttataaagg
     3661 ttacacaagt tgaggcttct caacaaaaga tagctaatat cgtaagaaaa cttgaaagag
     3721 aaggtgccat aactattgta aggaattttg gtggtgactt tgttgaataa taagattatt
     3781 aaaagtaaaa aagcattata taaaggagtg ctagatttaa ccataataga taaagttaga
     3841 gtgggagaat caaatgtaaa taaagaaaat gattgtagat ttagggatga ggtaataaaa
     3901 gaagctatag aatctgctga gataagaaag aatgagatat tagaagaagc aaaaatgaat
     3961 gcacaaaata tagaaaagca agcatatgaa aaagggtata gtcaaggtca aaaaaatgga
     4021 tatgaagatg gatataaaga gtcatatgat gaatataccc aaaaagctaa agatgaagca
     4081 gattttataa aaaatcaggc agacttaata ctattacaag ctaatgagaa gatgactgag
     4141 tacataaaag aaaaaaataa agagattgta agacttgcta ttaatatggc tagttctgtt
     4201 ctaaaaaagc atttcgaaga tgaagaatct atgagtgatt tgttaaagca aattattcaa
     4261 gattatgaag gaagtagttc atttatagta agatgtaatt ctttctataa agataatata
     4321 gagaaagagt ttgaattatt caaagaggaa acatcactaa aatcaagtgt atttgttatt
     4381 gcagatgata gtatagataa aggcaatgct attatccaaa aggaaaatgg aagaataata
     4441 gttggtattg attgtgctat ggaaaaacta aaagaagaat tgcttggtga ataaagtatg
     4501 tataaaactg atatatctat agattttgac aaaataaata agaaaataga aaatttagaa
     4561 ctcactatat cagaaggtag agtaaagaag ataataggac ttactgtaga agttgaagga
     4621 ataaaagctt ttgttgggga gttatgcgtt atatacaacc aagttaataa accagtgaat
     4681 tgtgaagtag taggttttaa agataaagaa gtcatattaa tggcgttggg agaattaaca
     4741 ttgatagcac ctggttgtaa agttatttct aaggggatac ctttaagtgt tatgtgtagt
     4801 gataatttac taggtaaagt acttgatggt cttggaaatc ctatagacaa ctcagatgct
     4861 gttttaggag atagatacaa cctgaacaat gaacctcctg acccaatgaa gagaaaaaag
     4921 atacgaaata taatggaaac aggtgtaaga gcaatagatg catttactac ttgtggtgaa
     4981 ggacagagaa tcggcatatt tgcaggaagt ggagtaggta agagtacgac tctaggtatg
     5041 attgctagga atgcaaaagc agatgtaaat gtaattgctc ttattggaga aagaggaaga
     5101 gaagtactag attttattga taaagattta ggtgaagagg gaatgaaaaa atctgttgtt
     5161 gtgtgtgcaa catcagataa agcccctcta gttaggttaa aaggtgctct tactgctact
     5221 gcaatagcag aatattttag agaccaaggc aaaaaagtaa tacttatgat ggattcagta
     5281 actagatttg ctatggcaca aagagaagtt ggtgtagcaa taggagagcc accagctcaa
     5341 aaaggatata caccatctgt atttgcaatg ttgccaaaac ttatggagag aacagggaca
     5401 tcagataaag gttctataac tgcattttat acagtattag ttgatggaga tgactttaat
     5461 gagccaatag cagatacaac aagagggata cttgatggtc atatagtttt atctagagac
     5521 ttggcaaata agaatcacta tccttctata gatgtattaa acagtttaag taggcttatg
     5581 aatgaaattg cttcaaaaga agacataaaa atagcgtctt ttgcaagaga catgcttgct
     5641 gagtatagag aagcagaaga tttgataaat ataggtgcat atgcttcagg tacaaacaaa
     5701 aaaatagatg aagcaatcta ttatcatgag catattatta attttttaaa acaaggaata
     5761 aatgaaaaga gttcttttaa tgaaactata agtagtttaa gaagaatctt tgaataaaca
     5821 atttaaaaaa atatttgaat aggttgtgat ggaattggat aaaaatttta agtttagatt
     5881 acagaaagtt ttagacttaa aaataaaaga tgaagaagag ataaaaatgg aatttgctaa
     5941 gattcaacaa aaaaaaatag atatagaaac taatttagaa aacttagaaa gtaattacag
     6001 caaatattct ataagtaaaa ataatgataa tgttcaaaat caaaaaataa ctattaacta
     6061 tttgctagct ttaaacaact ctataatgga tttatcagaa gaactggata agtcgacaaa
     6121 tgaacttgaa aaagctagaa aacagcttat aagcaaacag atagagagaa agtcattaga
     6181 aaaattaaaa gaaaaaaaat atggacagta ttataaagaa gaacagttaa aagaacaaag
     6241 cactaatgat gagtttgcaa gcatgagtta tttgagaaat agacaagttc tataaaataa
     6301 taggaagtga ggatattatt gtatgaagat acaagcagat tcttataata ttattaaaga
     6361 tattgggaat agaaaaaact catctaagaa taaaaatata aaaaataata aatttgaaga
     6421 taatctatat aagacaacag accataaaaa tgttaaagat agaagagaaa caagtaagca
     6481 aaatgttagt tcagatagac cgggaaatag tgataatgag tgtcatttag atgaagataa
     6541 aagtaatcac ttagaagatg aaaatacata tgaaaaagat tgtagtaaga ataatgaagc
     6601 tttacagcaa ttactgaatc ttttaaaaga gaattataaa aaagataaag atataaacac
     6661 tatattgaat aatataaaaa aactaagtat atcagatgaa ttaaaacaag aacttaagct
     6721 taataaaaat atattgaata aaattgcaaa tcaaaattta gagttaaatg gagctaatac
     6781 aaataaccct aatgatatag aaaatttaga cataatgaag ttaagagaac ttttagaaaa
     6841 taatgctagt gaagatattc aagtaattag taaaactaca gaaaatgata ctaaaagcat
     6901 agacttatac agttttgata gtaatagaat ggatactctt aactcaaata aacagaaaga
     6961 tgatagttct gatattttgg aaaaactagc agggttaaat ggaaataaga gtggaaattt
     7021 tggacaagta ataaataaaa gcgttgatat gaataaaatt aaaaatgata atgttaattt
     7081 aaaacagata gaatctaatg taatggatga ttctataaaa gctattaagc atatgaaaac
     7141 aaatgatata caagaattga caattaaatt aagaccaaaa gaattaggag atatgaacat
     7201 acaattatta aaagatggtg aaagtatgag agctgttgtg actgttttca ataaagatgt
     7261 ctttgactct ataaataaaa acatagcaga tttaaagcaa catcttgaac ttacaaatgt
     7321 aaatataaaa gatgtatcag tgcaaatgca tagcgataat acaaatacat ctgatacctt
     7381 tgataaagca tttgaacaac agaatagaca aaataaccaa gaaaacatga gtaatcaaaa
     7441 tacaaatgat aagcgtaaca atgtagtcat agaagatgag attaaagaaa atattattga
     7501 tgatgataga gtagatttac tagcttagta agggggaagt attgcatgta cggaatagca
     7561 acggctttgg caggaactca ggtaaactat aggtcaaata acttaagcca acttgaaaga
     7621 aatattaatg atattgccaa caaacaagtt agaagtaaag gaaatcagac agtaacagac
     7681 aatgggactc caattatagg acaaggtgat gatgctgata aagacttgtt tatgaagatt
     7741 cttgtagcac agatgggtaa tcaagaccca atgaatccac aagacccaac ggagtatgta
     7801 tcacaattag cacagtttgc aactttagaa aaaatgtcag ctatgaatga taagctagat
     7861 atacttttag ccttaagtaa ctctactcta ataaactctg ctttgagtat ggcaacatca
     7921 ttaattggta aagaagtaga agtatctggg gaagaagatg aagatgaaaa cattgtagga
     7981 gtagtaaagt ccacatacat aaaagatggg gaagtacact tagaattaga agtagatgga
     8041 agtgacaaaa ctgtagatgt taaatatgaa aaattactta aaataggaga ggtagaggca
     8101 aattcaggag aagaaaatga ctcttcagaa caagaatctc cagaagaagt ataatattaa
     8161 atgggaaaag aggtaaattt atgataaaag caatgtattc aggtgtaagt ggaatgaaag
     8221 caaatcaaac aaaactagat gtaatcggta acaatgtagc caacgctgga actacttctt
     8281 ttaagaaaag ttcagctaga ataacagatt cattttatca aactgtacta tatgcaagtg
     8341 ctccaacagc ggcattaggg ggaacaaact taggacaagt aggtgtaggt agtaagatat
     8401 caagtataaa caaagatatg acacaaggaa atgtacaacc aactaataga ccatctgatt
     8461 taatgataga tggagatgga tatcttccag tagtaagaca aggaacagtt atgtatacga
     8521 gagatggttc ttttaattta gatacaggat ttaaagcagc tgatggtgct ttgaagaata
     8581 ttaaaggtgg aagacttgta aatggtgatg gatatatgtt acaaggtatg atttatgatg
     8641 gggaatataa agctgatact ggagaatttg ttaaaaaggg tgatgctaaa ggaaaagagc
     8701 ctattgtaat accacttcaa agtataagtc ctaaggatgg aacagttcaa aatgtactag
     8761 agtataatat atcaaaggat ggaacagtag aatttttatt gagtgatggt caaagaactc
     8821 aatttgtcta tgatatacct gatggtgcta aggtaggaga tacacctgct ggaatagata
     8881 aattaaaagg tacaatacaa aaagtgcaaa catatgcatt ccaaaatcca gcaggactag
     8941 atgcagaagg aggaaacttg ttcaagccat cagcaaactc aggagaccca caaatagcag
     9001 gagctacagg cacaataata caaggagcta tagaagcttc caatgtagat atagcagaag
     9061 aatttacaga aatgataata gcaagtaggt ctttccaagc aaacagtaaa ataatatcga
     9121 catcagacga aatacttcaa gaaataataa atcttaagag atagtaggta catattatga
     9181 taagagtaac ggatttaaac ggaagaaaat acatattaaa ttcagatctt atagtgagaa
     9241 ttgactccgt gccagaaagc aggattctcc ttacaacagg agaaaaaaat cttgtaaaag
     9301 agtcagtaga tgagatagta gataaagtaa taaagtataa aagaaaaata aatgtaggtt
     9361 atattaggag tgaaaaataa tgaaaaaaac agattcacta acattcattg gactaatatt
     9421 atctacagta ttagttttat ttggagcagt aaaaggaagt agttcaggat tgaaaagttt
     9481 tttcgatttt gcatctatat taatcacagt atttgggtca tttggagctt tgatgattac
     9541 atttactatt gacgatataa aacttataaa agatgctcta caatactcat tcaagacaat
     9601 gaatatatcg aaattagact tattaaatca atttaaaaca ctttctaaaa aagcaagaag
     9661 agaaggactt ttatctatag aaagtgatgt aatgaaaata caagacccat ttatgaaaaa
     9721 aggattagag ttatgtattg atggtcttga aataaatgaa ataaggtcta tactagagct
     9781 cgaaatagat tctgttgaag aaaccaataa cagatattct aaactattta aaacttgggg
     9841 gactttcgca cctgcatttg gtatgttggg aactctaata ggtttaatac aaatgttatc
     9901 cgaccttaca tctccagatt taatagcatc tggtatggga aaagcattaa ttacgacatt
     9961 ttatgggtct ttacttgcca atatcgcttt gaacccaatt gcatataata tagatgagaa
    10021 gactgaaaaa gaaatttatg taaaggaaat gatgctggag gggataataa gcatacaatc
    10081 tggagagagc agcatagtag ttgaggaacg tttagctaca tacctttcaa ataatgagaa
    10141 gttagaaatt atgaaatcca ataaaaatac agaaagagca atgagtaatg gcgcgtaaaa
    10201 aaaataagaa aaaggatgag ataaatccag atgcatggtt ggctacttat gcagatacga
    10261 taacactgat tcttacattt tttgtgttat tgtatgctac atcagttgta gatatggaaa
    10321 aatttaaaaa tatagcagat gcgttaaaag gtcagttttc tgggacaagt atgtttagta
    10381 caggttcaag tacaagtgag aaatctccaa tagagagtgt tttagtggat aaaaatgata
    10441 agtcaaattt agaacaggat ttaaaagata aagttaataa ttctaactta caaaatagtg
    10501 taacagtcaa agatgatagt agagggattt tactagagct agatgattca attttatttg
    10561 actctgggat ttctgaatta aaagaagata gtaaagccac tcttaataaa gtatacgata
    10621 tgataaagat gatgaaaaat aagatagtta tagaaggcca tactgataat gtacctgtaa
    10681 aatcaagtac gcatgaatcc aattgggatt tatcttctag tagagcagta agtgttgtaa
    10741 gatactttgt agaagaaaag ggcatggacc ctaaactatt ttcggcaaca ggatgtggag
    10801 agtaccaacc tttggtatca aatgaaacag caaagggaag agctgagaat agaagagtta
    10861 atatacttat aactattgac taggaggaag aggtttgttc aaaaataaaa aaattattat
    10921 aattattttg atactgatta ttttctcagc aggagcaggt ttttttatat taggagaagg
    10981 tggaaataaa caaattgaca agattgcagg tttgtttatt ccaacaggga aaggcaaaga
    11041 tgaagtaaag accgaaaatc ttgacttaga agagatggta ttgaagctag ctgatgaagg
    11101 tgtaagatat ttaaaagtat caataactgt atcttatgat tcttcatata aagatataga
    11161 aaaaaatacg cctttaatta gggataatat tataaattgt tttatggcaa aaagggcaga
    11221 tgattttact gctgataatt taaagaacat aaaagaagaa attaaaaata cattaaatat
    11281 acaactaggt gaaaatgtaa ttcaaaatat atatttcaca aatatagtag tgcaataaaa
    11341 ggatggtaag acatgatact ttatttacta aaaatattaa tatgtatccc aataatttta
    11401 atattgatag tcttatcttt aaaaattagc caaggaactt tattaaaaaa atataatgat
    11461 aaatatataa aaatactaga tattgtaaat ataagtaaaa acaatagcat aataatttta
    11521 aaaataggtg aagaaggatg cattatggca tcttcttctt ctagcatgag taaaataaaa
    11581 gatttgacca aagaagaaat attaagaata gaaaacgaca ttaaagaaag aaattttaaa
    11641 attcaagatt ttaaaatgga aagaattaaa aagaaatatg atttcagtag atatattgga
    11701 aaatttacaa aaaaggaaga taagtaaatg gaactattat caaaattacc tttggctgat
    11761 gtaccatata caagttcaat gcaattgttt ttatttctaa ctgcattaat gtttttacca
    11821 tttgtaatgt tcatgatgac aagctttgtc agaatagtta taagtttatc atttttgaaa
    11881 tcagcattag gagcacagca agcaattcca agtcaaattt tggtagggct tgcaatagta
    11941 ttgacaatat ttataatgag accagtatta aatgagataa acgaaaaggc attacaacca
    12001 tatatgaaag aagaagttac tatggaagaa gctatgaagg aagcagaagg acctattaaa
    12061 gaatttttat taaagcaaac cagacagaca gatttagatt tatttgtaga gcaagctggt
    12121 ttaaaagaaa agaaattaac tcgtgaaaat ataccattat ctgttgtagt tccagcattt
    12181 gctataagtg aactaaaaac tgcttttcaa ataggttttt taatatatat accatttttg
    12241 ataatagacc ttgttgtggc aagtgttttg atgtctatgg gtatgtttat gttacctcca
    12301 gttatgatat cattaccatt taaattatta ttatttgtaa tggtggatgg atggaacttg
    12361 atagtaaaaa cattgatatt gggatttgga taggagaaaa atagtatgag tgaatttatg
    12421 ataatgagtg ttcttaaaaa tggcatgttt acaggagcta tgattgcagc tccagtgcta
    12481 attgtttctc ttgtagtggg gcttttaata agtgtatttc aagccacaac tcaaatccaa
    12541 gagcaaactt tgacttttgt accaaaacta atagcaattc ttttagtagt agctattggt
    12601 ggaagctgga tgttacatac tcttattagc tttacttcgg agatattcaa tatgatgtct
    12661 ctcataacta aataagtagg atatatatga tttttgtatt tattagaata gtagcattct
    12721 ttggaacatt aaggatacta tttccagctg gtacacctgc tttatttaag tcgctctttg
    12781 caatcattat atcaatcttg atttcaagta ctatgcaaat tgaatatgca acaaatatag
    12841 acaacattgt attgtttacc ttatatggta taaatgagac cataactggg attatgcttg
    12901 gatatataac caatctatgt ttttatagta tcagaatggc tggttctatg atggaccaac
    12961 agatgggatt atcaatgatt aatatgtttg acccaaattc aatgactcag acaactttaa
    13021 tcaataatct tatgaactgg acagcactta tgatattttt tagcatggat ggtcatcatg
    13081 ttttaattag aggcataagg tatagttttg agttaattcc aatagggaaa tcattcatag
    13141 ataataatat agattatata ataaacatat ttgtgcagtg ctttttaact ggattcaaaa
    13201 tagcaatacc tattgtactt tgtctgctta tggcagattt tatattaggg cttatatcaa
    13261 gaagtgtacc tcaactaaat gtaatgataa ttggtatgcc attaaaaata ctggttggta
    13321 ttgcattatt tatcatatca atacctttaa ttgtcaatca aataagtcat ttattaagtc
    13381 aaataccaaa gatgtatgag ggaacttttg cactagctcc tatgtttttt atgggttcaa
    13441 cagataaaac agaagaagct actccaaaga aaaagggaga acaaagaaaa aaaggtaata
    13501 tagcaaagag taaagaatta cccattgcaa tgactctact tgcattcact ttattagtac
    13561 ctacattatt ttcatatgta gtgaatacac ttaaatcttc tcttaattat tttttgagtc
    13621 tagattttta tatgaacata aattattcaa gtatagagaa attagttata gcaggattaa
    13681 tggatttttt taagatattt ttaccaatag ctattccatt cttagtttta ggtatagtcg
    13741 caaacctatt tcaagtagga atactgttta ctggtgaaac tttaaaaccc aatttatcaa
    13801 aattaaatcc aattagtgga ttcaaaaaca tgttttctat gagaagtttg tcaaccttaa
    13861 taaaagatac agcgattata tctattttgg cttacatagg atatacattc ttccaagata
    13921 attatttaga tatattaaag ttaggtaaca tatacctgcc aactttaatg tacacagtta
    13981 aagatttagt atattcaata ttaagtaaga tttgtgtagc tatgatagtt atagcagttg
    14041 cagattatgt ttatcaaaga tacagtcata aaaagcaact tagaatgaca aaacaagaag
    14101 ttaaagatga atataaaaac tcagaaggag acccagaaat aaaagctaag ataaaacaaa
    14161 aacaaagaca aatttcgtct caaagaacca tgcaagcagt accaagtgct actgtaatag
    14221 taaccaatcc cacccactta tctatagcag tgagatatga aaaagggaaa gaccaagctc
    14281 cagtagtggt ggcaaaaggt gcagattatt tagcttttaa aataagggaa attgcaaagg
    14341 gaaatgacat acctataata gaaaataaac ctattgctag gttgttatat aagcaagtag
    14401 aaatagacca agaaattcca gaggacatgt atcaagcttt tgcagagata ttagtagcag
    14461 tatataaaat caaaaataga tacaaagtcc ccaaaaggta ggataagtaa tgaataagtt
    14521 taatttaaag gaaaatatgg acgtaatagt ggcatttggt gttgtaggga ttgttcttat
    14581 gatgatagtg ccgttaccta catttatatt agatatttta ctggcaatta acataagttt
    14641 atctgtgcta attttactaa tgacactatt tacaacaaat gtacttgatt tatctatatt
    14701 tccaacagtg ttacttgtga caacactatt tagattggga ttaaatcttt catcaactag
    14761 attgatttta acacagggat atgcaggtga agttatagag gcatttggaa gttttgttgt
    14821 aggtggaaat tacgtagtag gaataattat attcttaatt atattaattg ttcaatttgt
    14881 tgttataacc aatggttctg gaagagtatc tgaagtaaca gctagattta cgttagatgc
    14941 catgccaggt aagcagatga gtatagatgc agatttaaat gcaggagtta tagatgacaa
    15001 aactgctaga aaaagaagaa aagagcttca gcaagaagca gatttttatg gctctatgga
    15061 tggagcaaat aagtttgtaa agggagatgc catagcaggt cttatagtaa cagcaataaa
    15121 cataataggt ggaattgtaa taggagctgt aatgctgaat atgactctta tgggtgcagc
    15181 acagacatat gtaagactta ctatagggga tggtcttgta agccagatac ctgctctatt
    15241 aatatctaca tcagcaggta taatcgtaac aagagcttct actgatgaaa actttggtgg
    15301 attagtaggt aaacagttaa cagcaatacc aaaagcagta atgatgactg gctgtgttct
    15361 aatcgtactt ggtatgatgc ctggattacc taaaccagca tttttttcac taggtatagg
    15421 tgctatagca gcaggttact ttttaggaag agaaaagaaa gatactttag aagacttgga
    15481 gtttgaaaaa cctgatatgg taaatccaga taaaaatgaa gtagaagatg taacaaactt
    15541 aataaatgta gaatcaatag aagtagaaat tggatatggt ttaatatctt tagctgatga
    15601 atcagcagga ggagatttac ttcagagaat agcatcaata agaagacaat gtgcaataga
    15661 catgggtata gtcgtacaac ctataagaat aagagataat ttacagttaa atccaaacca
    15721 atacaatata aaaataaaag ggaatgtagt tggtgggtat gagataatgc ctaatatggt
    15781 tttatgtatg gatcctatga atcaaggatt tagtatattt ggaataaaga caattgagcc
    15841 tacttttgga atagaggcac tttggataga aagtgacaag gtagaagatg cagagctaaa
    15901 gggttataca gttgtagatt catccacaat acttataact cacttactag atataataaa
    15961 atcaaatgca cacgaattac tgggaagaca agaagttaag actattatag atgcagcaag
    16021 agaaaattat agtgctgtag tagatgaatt gataccagat ttaatgacac ttggtgaaat
    16081 tcaaaaagta cttaaaaatc tgttaagaga aaaagtaaat ataaaggata gagttacaat
    16141 acttgagaca cttgcagacc agtctagaaa tacaaaagat atagagcttc ttactgaata
    16201 tgtaagaata gctttggcta gaagtatttg taataattta gttgatgaag ataaggcaat
    16261 tgtagtggct acattgtctc cagaaactga agaattggta tctaataatt tacaaaggtc
    16321 tgtaaatgga acttatccag cagttgaccc agaaaataca aataaaatat ttgagagtat
    16381 acaagaagta atgaacaatg tatatttcaa tcataatata ccagtaatag tagtatcacc
    16441 aaagataaga gctccattta gaaaattagt tgaaattgta tatcctaatt tgactgtatt
    16501 atcattaaat gaaataccta acgatattaa aatcaaagca gaaggtgtgg tgagcatata
    16561 tgatgacgaa aaagtataca gctaacacaa ttcaagatgc tatgaatcta gccaaaatgg
    16621 aattgggtga caatattaca ttaatagata aaaaagaggt aagaaaatct ggaataagag
    16681 gcattttttc aaagaaagat attgaattga ctattggatg ggaaaagaaa gataatttag
    16741 aacaaaaaga tttaaaacga gagatagaac aactaaagtc aattataaat aatatggggt
    16801 ttgataataa aaatgataat gatatagata aaatatgcaa gaatttacta aacttagatt
    16861 taaatcaaga aattgtagaa tttataaaag ctgatttaca agaaatgaag ttcaatggaa
    16921 ttgatacaag taaaaattta gtagaaagtc taaagaaaaa gattaaaata gaaaatcaag
    16981 caataaatgg taaaattgct ttagttggac caccaggagt aggtaaaacg actacaatag
    17041 caaagttggc tgccaaatta gtttttgagg aaaataaaaa agtaggagta ataactatag
    17101 atacatacag gattggtgct gtagaacagt taaagatata tactgatata atgaatattc
    17161 cttttaaagg ggtaataagc cctgatgaga tggaattggc acttgatgag atgaaagatt
    17221 gtgatgttgt attaatagat acaacaggta gaggctataa aaactctatg caaatattag
    17281 aaatcaaaaa tcttatagat aaagctgaaa ctgataatat acatttagtt ttgaactgta
    17341 caacaagaga aagtgataca aaagcaatta tagattcata tagaaatgtg aattttaaaa
    17401 gcttaataat tacaaagtta gatgaaacaa taacatatgg ctcaatattt aacataatga
    17461 attatgcaca aaaaccaata tcatatatta caacaggaca aaatgttcct gatgatatta
    17521 taaaacctaa tgaagataag attataagat tactattagg agttgaaagt atatgataga
    17581 ccaagctcaa attttgagaa aaatggcaat ggaaaaaaga ggttctgatg agattatagt
    17641 agagaatgag aatatgccta agataattac aatagcttct ggtaaaggcg gcgttggaaa
    17701 gagtaatctc gctacaaatt tgtctatatg cttaaccaaa cttgataaaa aagttcttat
    17761 attagatgca gatataggaa tgtctaatat agatataata atgggggtaa atgtaaaagg
    17821 gactataatt gatgtaatta atggcgaaaa aaatatagaa gatataattt cacagactaa
    17881 gtatggtgta aatataatat ctggaggttc tgcccttaat catatagaag actttacaga
    17941 agctcaaaga aataagttta tacatagcat agaacagatt cacaatgtgg attttataat
    18001 aattgataca ggagctggta tgagcaaaag cctattgtca tttatatact gtagtactga
    18061 atttttctta ataacaactc cagaaccgac atcattgact gacgcatata gtttattaaa
    18121 agctataagt aattttggca taaagaaaag tgcaaatata ataatcaata gggtaattga
    18181 tttagaagaa gcgaaatcta catacaaaag aataaaaatg gttgtggaca aatttctaaa
    18241 ccttaatctg gaattgtatg gatatataat agatgacaaa aaagtaggtg tatgtgtaaa
    18301 aaaacagata ccattcattt tagaatatcc tacaagtatt gcatcaaaat gtattatagc
    18361 tatagcaaag agaatggtaa gtcagtcaag agaagatatt gttgataata aaagttttct
    18421 aagaaagatg tttagtatat ttaaaagcta aggaggcgta gttaatgaat agagaagaat
    18481 taataaaaga gaatatgcct cttgtaaaga gtatagcaag aaaatttttt ataccaggga
    18541 aaggttttga atatcaagac ttagtaaata gtggagtcat tggattgatt gatgcaataa
    18601 ataaatttga tactgaaaaa ggagcaaagt tttctagtta ctcttatata aagataaaat
    18661 ctgctatatt agatgaaatt agaaatcaaa gtccaatttc taaacataat ctaactaaag
    18721 taaataagta taatagagtg gtagaaaagt tgcaatctaa attattaaga gagccaactt
    18781 catatgaaat tgcaaaagaa ttaaaggttt cagaaaaaga acttcatgat atcgaaagta
    18841 atatagatat gttaaatatt gtatcattaa actatgtaat ctttgaagat acaaatgaaa
    18901 ctgtacaaga tgtaataagt gatagagaag aggaagctcc agaaaatata atagaagaag
    18961 aagaaaagtt agagattcta tccaaagcaa ttagtaattt aaatgaaaga gaaaaattaa
    19021 tactttcact gtactattat gaagatttaa atctaaagga gatagggaaa gtgctaggtg
    19081 tttcagaatc aagagtttca caactccaca gaaaatcaat aagaaactta agaaacaaga
    19141 taaaagaact taaatattct atatagatgg tgattgataa gatgatacaa attatattaa
    19201 tagtagtgtc tattttaata atagtaacta tagttataaa tttacagaag gaatcaaaaa
    19261 ataaagataa cagatacttt gataaaatat ttgagcaaga atatgaagca aatgatagat
    19321 taataattgc agaaaaaaga agattctttt tagacaaagt acttgcagaa aaaagaatta
    19381 atagtcatat aaaagtttat gaaaattata acctaataaa ggctaatgaa gaaagaaagg
    19441 aaaacatatt tatagaatca aaaaaggaag taagacaaga aaatgagtta tctcttaaag
    19501 tgaaacatct tataagttca ggttatgatg atgtggaaat atgtaagatg ctagatattg
    19561 gaaaggggga actgttatta ataaggagtt gttacaaaat ataaggatat tattatgtga
    19621 taaaaaagta ttcttaggaa taggtattgg tatattaata tcaactttat gtataatgct
    19681 ttttagtaat aatgatatga gtaaggcaga gatagaaaaa aaggccagag cgttaggcat
    19741 gaaatataca agtgagatga aagttttaga atagatgata ctgtttagac atttacaaaa
    19801 aaggaggtca taatgtgata agaggattat atacagcagt ttctgctatg ataactaatc
    19861 aacaaaagca aaatgttgtt gtcaataacc tagctaatat ggatacaaat ggatataaaa
    19921 gtaaacaact tttgactaaa agttttgatg aattaaagtt ggagggttac aataactacg
    19981 caaatggaac taaacaaaag cagattatag gcgatataag tccaggtatt tctatggatg
    20041 aaactatcac aaattttaat caaggaccta taaaaactac tgataataaa atggatgtag
    20101 ctattcaagg taagggattt tttctagtta gtgatgcagc tggaaatcaa ttttacacta
    20161 gaaatgggaa cttcagaaca gacaatcaag gtgaccttat aacaagtgaa gggtattatg
    20221 tacttgggac gaatactgct actggtgctg taggtcatat aaatgtagga aatcaaaaat
    20281 ttgaagtggc aaaagataat acaatatcac tagacggaaa tgctatgtat aaatttaata
    20341 ttgtagattt taataactac aataacttaa aaaaagaagg agataactta tattctggag
    20401 gaggagctat gaaagtaaat aatgcactta caatacaaag tgcgatagag tcctctaatg
    20461 tagatatggt atctgaaatt aataatatga tgacaatatc aagagaatat gaggcaaatc
    20521 aaaagataat tcaggctatg gattctaaat tagcaaaaat agcaagtgag attggttcag
    20581 taagataggt aataggaggt gaatttttat gtacacaatg ttttatacta gtaaaacagc
    20641 tttatcagca aatcaaagta aactagatat aatatctaac aatatagcaa attctgattc
    20701 aacaggatat aaaaaaataa atatggaata ttctgattta gtggatgaag tactaaatag
    20761 accatcatac cctacaaatg gtaaagatat atcgactgga acaggagcta aggcaacagc
    20821 tcccatgaga aattatgaac aaggtgcatt ggctcctaca gatagtaaga gtaatttggc
    20881 gatagatgga gaaggattct ttagagtgat aagaaaagat ggtacatatg catatacaag
    20941 aaatggtgga tttaatgtag atgctttagg taagattgta gatgataatg gaaatatatt
    21001 agatgtccaa tttgatgcag gatataacta taataataca aatatagata gtacaaacct
    21061 gacagtttca agaaatggag agctattcgt agataataaa aaaataggaa aaattaactt
    21121 atatcaacct ataggaactc aaaattttat atctgaagga gatagtttat ttgtcgcaac
    21181 tgatgcagca cagattaaac aagttgagaa agtcgatata gttcaaggct atagagagag
    21241 gtcaaatgtt tctttacaag aagaatttgt tgacttaata gccactcaaa gagcatttca
    21301 aatgaactca aagggaataa agacagcaga tgaaatgtgg caaatagcga ataatttaag
    21361 agctaaataa ttatgtaagg agaggaatat tcatgaatga tatgaaagag tacgattttg
    21421 gcaaacctca gagttattca agagaaaatt taacttgttt aaatttttta ttagctgaat
    21481 tttgtaaaaa atataaaaac tatataattt atgaacttaa gtgtaattct agtatggttg
    21541 ttgatagtat agaccaagta aattatcaga cttttttaga tagagtaact tcttcatgcg
    21601 tagtagtaca aaatgcaatg gaacctgtta tgaaaaactt ttcttttaga atagatagaa
    21661 gtgtagctga tatgtggatt gatataactt ctggaggaac tggagttgta aaggataatg
    21721 atagagagct tacagaactt gataaaaaag tgcttttaca ccttatggat gatttagtaa
    21781 gaaatatgca cttatttgag ggttttgaaa atatacaagt attggataca catacacata
    21841 ttaatttgcc acaattatgt tctccaactg caccagtatg cgttgtagat ttaaaagtgc
    21901 taaatgataa tgagtgtatt ggaagtgttt ctctttgttt tccatacaat ggtttagagg
    21961 ctctgttaga aagtatatct gttatagaat tttttgataa tgatgtagga gatgattcag
    22021 aggaatttac ccaaaaaatc tataatagtg tatctaatat agaattatca gttattgcag
    22081 agataggaaa agtatcaatt aatgttgagg atttacttaa actagaagtt ggagatgtaa
    22141 tagtaacaaa taaaaaaata aatgattata tagatatatt tgtagaaaat tcaaaatcat
    22201 acacagcaac accaggattt atatcaagta aaaaaggtgt aaaaataaat gatgctgttg
    22261 gaaaagaggt gtagttatgg acaatagtaa tcttatttca gatgaagaag caaagatgtt
    22321 attaaatgat aaagttgaaa gtgaagttga tggagatgat gagttagcct tagatgtctc
    22381 tgaagttaat cgaaataata tacaaaggat acttgacctt aagttagaat tatctgtagt
    22441 tataggaaga actaagatga ctctaagaga tattttaaat ttacataaag gttctcttat
    22501 tgaactggat actttagcag accaagatgt ggaaatacta atagataata aggttttggc
    22561 ttatggaaaa gtagtggtgg tagatttaaa ttttggtgta aagataacaa gtatagtaag
    22621 tgaagaagac atggtaaaat ctcttgttta gagataacat caattaatac ttaaggcatg
    22681 aggtggtact tattttaaac ttaaatgata taaaaaaaga tatactaaaa ctcaataaaa
    22741 aagactcatg ttctctatat aatgaatcta ttaataatat aaataataat tgtatatcaa
    22801 ctggattaaa atttttaaaa caagcaagag atttaaaccc agatgatgtt gatatattaa
    22861 atctcacagg tcttgtaaac ttactaaaat gtaattttga tgatgctgta gaaagtttct
    22921 ataagagttc actttgtgaa aaaactgctc tgtgtgaaaa gtatataaac atgcttacat
    22981 ctgaggagtt tttaattttc tttgaaaagt acaatcaact tataaggtta ataaataaag
    23041 gaatgtataa agaagctata caagggttta aactcataac tgaccaatac tgtgatttaa
    23101 tagaacctta tgagttgctt gccttactgt atgacaagga agaagaatat ataaaatttg
    23161 atgaatgttt agaagtattg aagattattg ataaggaaaa ttatttatta aatcaagaaa
    23221 ataacacatt agatatgtat aatagtgcta atatacaaac taaaagtaat ataaaagtag
    23281 atgattataa gaatgagttc ttacataaaa gtagtagtaa ccataaaatt aatgattata
    23341 atgatgtaac taaagaaaag agttcaagca aaaaccagaa aagttcagat tctaataaga
    23401 aaaaaagtat taaaaaaaat aagtacctat atatagccat aatattagga atattattag
    23461 taggacaaag tatatataat tcttataaga tgaataattt aactgataaa atatctaaaa
    23521 ataatgaaac taaaaatgaa gagtcaatag attctaactt gactaagaaa gaaaataata
    23581 tagaagaaaa tgaagatatg agtaaggctt ctaatataaa taagagtaat aaaaacatta
    23641 ataaagagga tgatgtaaaa tctaaaaaaa atactgtaga aaatgaagat gataaagatt
    23701 ctgacttatt tacagaggat gaattgatgt ctaaggcgaa tactttaaaa tcagaaaaga
    23761 aaactaaatc ttctatacaa ctatataaga aagttgcaga tataggtgaa aataaaggga
    23821 atacatctga agcaacttat caagttgcta ttctcagtga aaagttaaaa gactataaaa
    23881 ctgctgaaga atattataaa atgtatgtag aaaattactc agaaaaagat gcttattttg
    23941 atgaatctta ttataattta ggtatgatgt actataacaa tggtgattta aaaaattcta
    24001 aattgacatt aaagaagttg gttaataagg taccaaatag tatgtataat aattcaaaag
    24061 taaaagaaat tttaaaagaa gaatagttaa attagataag aaaaataaat tattttattt
    24121 taaaataata tgattataat atattgacat accattttaa aaaatttaaa atattaatta
    24181 taaaatgagg agggataaaa aatggaattt gaaaaaggga gtatttcaat tcatacagaa
    24241 aatatttttc ctataattaa aaaatggtta tactctgata aagacatatt tataagagag
    24301 cttataagta atggatgtga tgcagtaagt aaacataaaa gacttgtatc actaggagaa
    24361 atatcagaaa ataagtcatc agattataaa attacagtat ctgtaaataa aggagaaggt
    24421 acacttaaat ttattgacaa tggaattggt atgactgaag aagagataaa aaaatacata
    24481 aatcaagttg ctttttcagg tgctgaagat ttctttaata aatataagga taaaatggaa
    24541 gagtcaaatg atataatagg tcattttgga ttaggatttt attctgcatt tatggtgtct
    24601 aaaaaggtac aaatagatac actttcatat acagaaggag cgactcctgt aagatggata
    24661 tctgaaggtg gtacagaata cgaaatatct gaatcagatg ctagaaatga tagaggaact
    24721 actataacac tatttattga tgatgatagt aaagaatttt tagatgaatt tactgtaaga
    24781 ggaataataa ataaatactg ttctttctta ccagtagaaa tatatttaga agatgttgaa
    24841 agattagaaa gagaagctaa agaagcagaa gaaaaagcta aaaaacaaaa ggaagatgga
    24901 aaagaagaga ttgtagatgc aaaagtaatt gagcctttaa acgacactaa tcctctatgg
    24961 ttaaaatctc caaaagactg tactgatgag gagtataaag agttttatag aaaggtattc
    25021 aatgtatttg atgaaccttt attttggata catttaaatg tagattatcc tttcaattta
    25081 aagggtatac tttacttccc taaattaaaa aatgagtttg aactgactga aggaaaagta
    25141 aaattataca acaatcaagt gtttgtggct gacaatataa aagaagttat accagaattt
    25201 ttacttttat taaaaggagt tatagactgc ccagacttac cattaaatgt atcaagaagt
    25261 ttcttacaaa atgacaggga tgtaagcaaa atttctaaac atataattaa aaaagtagct
    25321 gataagttaa aatctctttg taaaaatgaa agagaagaat acaataagtt ttgggatgat
    25381 atacaaatat ttattaaata tggttgctta aaagatgaaa gtttctatga gaaggtaaaa
    25441 gaatgtatat tattcaaaac tattgatgat gagtatataa cattacaaga ttatttagaa
    25501 aagtgtaagg acaaacatga aaataaggta ttttatgtaa gtgataaaga acagcaatct
    25561 caatacatta aactatttaa agaatacgac ttaagtgctg ttgttttaaa ctcatcaata
    25621 gatactcatt ttatttcatt tatggaatat aaagagaatg gagttaagtt taatagaata
    25681 gatgcagacc tttctgatgt attaaaagat aaaaatgaaa ataaagatag tgaagaaaac
    25741 aaagaagaga tagctaagat agaaggtctg tttaaagaag ctgttggaga aagagtgaag
    25801 aattattctg tagaaggatt gaaaaatgaa gacactccag ctatggtgtt agtatcagaa
    25861 caatctatta gaatggcaga aatgcagtca agatttgctg gaatggattt aggaatgaat
    25921 tttgaagaag aaaaaactct tgtaataaat gagaacagtc caataatcaa aaaattggtt
    25981 tctcttaaag atgatgagga aaagaaagat aagattacgc ttatttgtaa ccagatagca
    26041 gatttagctt tactttctaa taaagaatta aagccagatg agttagattc atttgtacaa
    26101 agaagtaata agcttatgag tcttttgata gaattataag attgaatttg aatacattga
    26161 agcaaattta taaaaagtat tcaatatcag tgataagaaa gaagtgtctc aaaatgaact
    26221 tttcagttca tgaggcgctt tttttatgtg aaattaaatt tattttaatc atttcaacaa
    26281 tgaaaaatat atttatctct attttgagac agaccgttct ttattttttt atctttggtt
    26341 aatttttaaa tatttaagta ggttctaata aaaaactcta tttagttaaa tatagttaac
    26401 ttgatatata ataagataat acatataatt aagtaaaaga ttgtttttta gtttatgttt
    26461 aaatacataa ttacactttg ttaaaaacat aaaattatta ctaaagtaga aataggaggt
    26521 tttagcatga attttaatta taatttgcca gtaaatatct tatttgggag cggcagaatt
    26581 aatgatgtgg gaaaagaagt atctaaatat ggcaaaaaag tattgatagt aacaggtaaa
    26641 aatagtacaa agaaaactgg attacttgat aaaacaatag atttactaaa agactctaaa
    26701 atagaatatg aagtttttga tagagtagaa caaaatcctc ttataactac tatatattca
    26761 ggagtagaaa ttataaaatc aactggatgt gattgtgttt taggtcttgg tggtggaagc
    26821 attatggatg ctgccaagag tatagctttt tcttataaaa acccaggaga tttaaatgat
    26881 tatatatttg gaattagaca aggtgaagaa gctttaccta taatcttagt tccaacaaca
    26941 tcaggtacag gtagtgaagg aaattgtttt gcagtactta caaattcaga aactaaagat
    27001 aaaaaatcta taaagaagaa tagtatgtat gcaaaagcat caataattga cccagaactt
    27061 atggttacaa tgccaaaaca tataattgca tcagttggat ttgatgcact tgcccataat
    27121 atggaagcat atctttctaa tggaagaaac cctcttgcag atgtacaagc tatttatggg
    27181 attgaactta tatcagaaaa cctaataaag gtatataatg atgttaatga tttagaagca
    27241 tgggaaaaaa taacacttgc aagtactatt ggaggaatga cgattggtac agcgggtaca
    27301 gccttgccac atggaatgga gcatcctgta agtggtctta gagatgtaat gcatggaaaa
    27361 ggacttgcag ctttgactcc tactattata gaaagttcat gggatagtga tattgaaaga
    27421 tttggtaata tttcaaaggt attaggagga acttgtgcag aagattgttc agatgcaatt
    27481 agaaatttcc ttaaaaaaat agatttagat ataaagctaa gtgatttagg tattgaaaaa
    27541 tcagatatag attggttaac tgataattgt atgaaaatat cagttgcaaa tattaagaga
    27601 catccaaagt attttaataa ggaacaaata aaggaaatgt accataagtc tttataattt
    27661 gactttacat atttagtttt atataaaaat tataaattaa taataaaaaa ttataagtaa
    27721 ttaatagtaa aaaaattgtg tatagaagtt atatatataa taaatttgtc ttaagctttt
    27781 attttacttt ctaataaata aaggagttta aaaaagggaa gattaattaa gtaatatttt
    27841 ataaaattct tcgatttaaa aactttaagt agaggtgaaa taaatgttta atcccaaaag
    27901 aataatattc gaaaaaggaa ctttagacac tgatgtgggt aaaaaaatat acaataaatt
    27961 tagagataat aaagatgtag agataattaa tatatcatca aataggatta aagaacatat
    28021 tccaggtgaa aatctatatg aacaatatag aagtgggaag cagactatgg tagttgggat
    28081 tagaaaaagt ctgaagtttc aaacatgtaa accttctgct aattatcaac taccacttgt
    28141 cagtggatgt atgggaagat gtgagtattg ttatttgaac acacaattag gtgataaacc
    28201 atttataagg gtgtttgtaa atgtagatga aatacttgaa cagacaaaaa aatatattga
    28261 agatagaaag ccacaggtaa ccatatttga aggggcagct acatcagacc cagttccagt
    28321 agagccatat actaattctt tgaagaaaac aatagagttt tttggaagag aggaaaaagg
    28381 aagatttaga tttgttacta aatacagtga tgtggatagc ttattggatg ctgaacataa
    28441 taatcataca gaaatacgat ttagtatcaa tactcctaga attataaatg aacatgagca
    28501 ttatacagcc tctgcaaata aaagaataga gtcagctgta aaacttgcta gggctggtta
    28561 tcagattggt ttcataatag cacctgtgtt tttatatgaa ggttggaaag aagagtataa
    28621 aaatctgatt ataagtataa aagaagcttt accaaaggat tttgataaaa aaattatatt
    28681 tgaagttata tctcatagat atacaactaa agcaaaaaat agaatactag agatttatcc
    28741 agatacacta ttgccaatgg aaaatgaaga tagaaagttt aaatacggtc aatttggata
    28801 tggtaagtac gtgtatgata aagaacaatt agatgatatg aaagagttct ttcttaagga
    28861 aataaatgga gtatttaaaa atagtttaat aaaatatata atatagcaaa tcttacctaa
    28921 agatttgcta tattttattt ttatatattt tatgtttata ataaagcaat gagatttagc
    28981 attatcatat aagaaaatta aaaagggcac tatctatcaa gaacattttt tctagttttg
    29041 atactataaa ggataagagt aacaaaaata tcaggaggag ttatagtatg catgcatggg
    29101 agtctattca aattacattg gatttaatag aagcaaattt atcagaagaa atatcaatta
    29161 atgaattagc aaataaagca aatttatcac cattttacta tcaaagacta tttaaaagat
    29221 tggtaaataa gactgtaatg gagtatataa agcttcgtcg tcttgcaaga gcttctgaat
    29281 acttagcaga acataagaat cgtatacttg atgtagcttt aaattttgga tttgctagtc
    29341 atgagacatt tacacgttcc tttaaaaaag catatggatt aactccagaa aaatatagag
    29401 caaatccagt aaaactcaat cattttatta agccagaatt aattttaaat tatgctatgg
    29461 ttgatgaagg tgttccactg attgcagatg atatagtgat tgaagtcaat cgtaagacat
    29521 tgagtaatcc aaggacattt gttggaatag aaatagaagt acctatttgc cagttggtgg
    29581 gcggtgaaac aactggaatt gcaatagtag aggaattatg gatgaaactt ggaagtcaaa
    29641 gacataatat accaaatcaa attccaggtg gaaatgaatt tgctgctctt tatatgggaa
    29701 atgcaaaaga aggaaattgt atgtatatgg caggtgtaga ggtagagtca ggtacatcag
    29761 tagaaggata tagtactttt gagttgccag ctaaagaata tttagtatgt ggatttgagg
    29821 cagaaaactt taatgagtta gttaattcag ctgtttttaa agcagataaa tttatggaaa
    29881 gatggatgaa gaaacataat ttaacaacta ctgattttgc aattgagatg tattacccca
    29941 ctacacctga agcagcatat ctggaacatt ggattgttcc ggttcctatt gaacaataga
    30001 aaggatgatg aatatgaatt ggcaagatgc gttttttcaa gatactaaac ctacatttga
    30061 gcaaattaat gaatttatta ataatccatt atgggacaag ttaaataaaa ctttggtttc
    30121 tacttataat gtagaaccta agcttgaata cagtaaatgt tcaatgcaaa gaggttggaa
    30181 tgtaaaatat aaaaaaagag gtaaatcact atgtacttta tatccacaag aaggttattt
    30241 taaagctttg gttattgtaa gtgaaagcaa tagagtagag gttgatttat ttattaatac
    30301 ttgttgtgat tatatcaaaa aaatatataa tgaggtaaac ttttttaatg gaagtaaatg
    30361 gctaatgatt caagtagata gtttattggt attaaacgat atgcttgaac taattaaatt
    30421 tagagcttaa catatagaac aggatgaata tataagtcat cctgttttta tattcaatca
    30481 gataaattaa tatatagtct ataatatgaa taatttagta aatttaaatt tactttatct
    30541 ttctacattc tttgcaacta agtccacatg tgtttttttc ttgcatatta gtagcacccc
    30601 aattagcaaa ttgttgtaaa ataggcacaa tacttttacc tttacttgtt aatgtatatt
    30661 caacttttgg aggaatttca ttatattgtt tacgatgaac aataccagta gactctaaat
    30721 cttttaatga ttgagctagc atagtatttg taatgccatc caattttctt tttatgctac
    30781 tatatctaat tggattttca actgctaaca tacatataat aggtaatttc cacttaccac
    30841 cgacaatgtc taatgcataa cggattggac aggttaattc ttcaacgttt ttagttatat
    30901 ttttttcagt atttatactc attttaatta ctccttagtt atagtagtat cttttttata
    30961 ctttatcaga aaaaactgca tacttgtttt ttctgtattt tatattaaaa ttatatcaaa
    31021 ggataatcct taatacaagt atttaaaagg ggtgtaagaa ttatgaaagc attagaattt
    31081 ttgaacaagg caggagttta ttatttagca acagttgatg agaacaatca agcacatgtg
    31141 cgtcctttag gatttgttat ggaatacaat ggtaaactta cattttgcac atctaatcaa
    31201 aaggatatgt ttaagcaact tatttttaat ccaaaggttg aactttgttg tatagaccaa
    31261 aaccttaata ctctacgtat tttaggtgaa gcagtatttg ttacatcaaa agaaacacaa
    31321 gctaaggcac ttgaaataat gccaagttta ggtcagatgt actctgtagg agatggtaag
    31381 tttgaaatat tctctattga taaggcaaaa gttagttgtt gttcaatgag tggtaaaaag
    31441 attgatgtag agctttaatt ataattgaat cgacatccat agcgaaaatt aagaaaacgt
    31501 tattactgaa atattgatag atttaattta aatgcaataa aaagattttt aactaatgaa
    31561 tatgtaaaaa tttttagtta ttagatgtgt atttcaataa tgatatacta ttttgaataa
    31621 ttacatacta gaaataagat aattaaaatt tactttataa tgggatagat ttcttttaaa
    31681 aaatatttta actataattg atttttattt aatctgaaaa aaatat
//
LOCUS       DAYATN010000032        31560 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.032, whole genome shotgun sequence.
ACCESSION   DAYATN010000032 DAYATN010000000
VERSION     DAYATN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.032"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1637
                     /locus_tag="ACGI8A_001449"
     CDS             <1..1637
                     /locus_tag="ACGI8A_001449"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFH0179452.1"
                     /translation="KMKEYILISCKYIDKCLPKDYKFLARFYSQIAILSLKNEDVNKA
                     NLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIETLYIKIKEINDF
                     NCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLCNIKIGECECSF
                     ENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVAINNLAKSEVYL
                     EKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILNMIKNIEEYIIY
                     DLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKSIYLCFEKIQNY
                     EMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVKLDLDKKKYVDH
                     LTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDIALKNITIIIKK
                     YLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNLTVSIGIDTCKN
                     SSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            1721..3551
                     /locus_tag="ACGI8A_001450"
                     /pseudo
     CDS             1721..3551
                     /locus_tag="ACGI8A_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            3559..5559
                     /locus_tag="ACGI8A_001451"
     CDS             3559..5559
                     /locus_tag="ACGI8A_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0179453.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            5821..7080
                     /locus_tag="ACGI8A_001452"
     CDS             5821..7080
                     /locus_tag="ACGI8A_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0179454.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            7141..8928
                     /gene="iorA"
                     /locus_tag="ACGI8A_001453"
     CDS             7141..8928
                     /gene="iorA"
                     /locus_tag="ACGI8A_001453"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HFH0179455.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            8930..9505
                     /locus_tag="ACGI8A_001454"
     CDS             8930..9505
                     /locus_tag="ACGI8A_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HFH0179456.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            9558..10628
                     /gene="buk"
                     /locus_tag="ACGI8A_001455"
     CDS             9558..10628
                     /gene="buk"
                     /locus_tag="ACGI8A_001455"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFH0179457.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            10846..11547
                     /locus_tag="ACGI8A_001456"
     CDS             10846..11547
                     /locus_tag="ACGI8A_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0179458.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            11607..12113
                     /locus_tag="ACGI8A_001457"
     CDS             11607..12113
                     /locus_tag="ACGI8A_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFH0179459.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            12288..13310
                     /locus_tag="ACGI8A_001458"
     CDS             12288..13310
                     /locus_tag="ACGI8A_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFH0179460.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(13457..13765)
                     /locus_tag="ACGI8A_001459"
     CDS             complement(13457..13765)
                     /locus_tag="ACGI8A_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HFH0179461.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(13825..14253)
                     /locus_tag="ACGI8A_001460"
     CDS             complement(13825..14253)
                     /locus_tag="ACGI8A_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179462.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(14476..15933)
                     /locus_tag="ACGI8A_001461"
     CDS             complement(14476..15933)
                     /locus_tag="ACGI8A_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFH0179463.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            16351..17265
                     /gene="nadA"
                     /locus_tag="ACGI8A_001462"
     CDS             16351..17265
                     /gene="nadA"
                     /locus_tag="ACGI8A_001462"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFH0179464.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            17278..18579
                     /locus_tag="ACGI8A_001463"
     CDS             17278..18579
                     /locus_tag="ACGI8A_001463"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFH0179465.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            18560..19396
                     /gene="nadC"
                     /locus_tag="ACGI8A_001464"
     CDS             18560..19396
                     /gene="nadC"
                     /locus_tag="ACGI8A_001464"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFH0179466.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            19652..19984
                     /gene="saoD"
                     /locus_tag="ACGI8A_001465"
     CDS             19652..19984
                     /gene="saoD"
                     /locus_tag="ACGI8A_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE-related protein SaoD"
                     /protein_id="HFH0179467.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            20074..20331
                     /gene="saoT"
                     /locus_tag="ACGI8A_001466"
     CDS             20074..20331
                     /gene="saoT"
                     /locus_tag="ACGI8A_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_240067706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:20107..20109,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-like (seleno)protein SaoT"
                     /protein_id="HFH0179468.1"
                     /translation="MSKILVEFINTUPSCDEHGVFIKKMSEKYADKLEIKLYQAGKDF
                     SYIKKYGIITKGTLIINQKKKYDRLNKDTIERAIVEAINNN"
     gene            20358..21443
                     /gene="saoE"
                     /locus_tag="ACGI8A_001467"
     CDS             20358..21443
                     /gene="saoE"
                     /locus_tag="ACGI8A_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter SaoE"
                     /protein_id="HFH0179469.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            21436..21894
                     /gene="saoC"
                     /locus_tag="ACGI8A_001468"
     CDS             21436..21894
                     /gene="saoC"
                     /locus_tag="ACGI8A_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Cys-COOH (seleno)protein SaoC"
                     /protein_id="HFH0179470.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            21929..23020
                     /gene="saoX"
                     /locus_tag="ACGI8A_001469"
     CDS             21929..23020
                     /gene="saoX"
                     /locus_tag="ACGI8A_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding subunit SaoX"
                     /protein_id="HFH0179471.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            23034..23822
                     /gene="saoB"
                     /locus_tag="ACGI8A_001470"
     CDS             23034..23822
                     /gene="saoB"
                     /locus_tag="ACGI8A_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:23247..23249,aa:Sec)
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     (seleno)protein SaoB"
                     /protein_id="HFH0179472.1"
                     /translation="MKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTSGFV
                     INYMVNRDYFENIKVSDIMDIFTINDCUASTMQWALSSEEIDIAIICKDAAKQYVNVN
                     SGFEIVGTVVQNSDIFLLSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAY
                     GLESNLVDGVVIDCIKSIGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYN
                     KSVDALKNKATFKKVLKEYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            23846..24589
                     /gene="saoP"
                     /locus_tag="ACGI8A_001471"
     CDS             23846..24589
                     /gene="saoP"
                     /locus_tag="ACGI8A_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591060.1"
                     /note="Most members of this family are selenoproteins with
                     the selenocysteine residue at the channel-gating position;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:24410..24412,aa:Sec)
                     /transl_table=11
                     /product="ABC transporter permease subunit SaoP"
                     /protein_id="HFH0179473.1"
                     /translation="MDILYKIILLVIIFLVWHFAAKDIGSSLLLPMPVDVIKGFFFCV
                     TDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFSKTFERVLSPVVDSVRQVPIMA
                     WVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVRAISKDYYNAARSMGASPIVIF
                     TNVIVPASLPDILTGSRIAISTGWMSVIUAEFIATSAGLGYSMVQAQTLMHTDVLIGL
                     MIFAALIGFIIDRVLKFINKILCRWRFAD"
     gene            24589..25341
                     /gene="saoA"
                     /locus_tag="ACGI8A_001472"
     CDS             24589..25341
                     /gene="saoA"
                     /locus_tag="ACGI8A_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein SaoA"
                     /protein_id="HFH0179474.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            25354..25995
                     /gene="saoL"
                     /locus_tag="ACGI8A_001473"
     CDS             25354..25995
                     /gene="saoL"
                     /locus_tag="ACGI8A_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_238476746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:25837..25839,aa:Sec)
                     /transl_table=11
                     /product="MerB-like organometallic lyase SaoL"
                     /protein_id="HFH0179475.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     CUNIMNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLKQKVLS"
     gene            complement(26131..26952)
                     /gene="yidA"
                     /locus_tag="ACGI8A_001474"
     CDS             complement(26131..26952)
                     /gene="yidA"
                     /locus_tag="ACGI8A_001474"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFH0179476.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            27445..29139
                     /locus_tag="ACGI8A_001475"
     CDS             27445..29139
                     /locus_tag="ACGI8A_001475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFH0179477.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYIRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      29471..29581
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            29685..30056
                     /locus_tag="ACGI8A_001476"
     CDS             29685..30056
                     /locus_tag="ACGI8A_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HFH0179478.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            30151..31098
                     /gene="trxB"
                     /locus_tag="ACGI8A_001477"
     CDS             30151..31098
                     /gene="trxB"
                     /locus_tag="ACGI8A_001477"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFH0179479.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            31202..31519
                     /locus_tag="ACGI8A_001478"
     CDS             31202..31519
                     /locus_tag="ACGI8A_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HFH0179480.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT        12498 a         3244 c         5611 g        10207 t
ORIGIN      
        1 aaaaaatgaa agagtatatt ttgatttcct gtaaatatat tgataaatgc ttacccaaag
       61 attataaatt tttagcacga ttttattcac aaatagctat tttaagttta aaaaatgaag
      121 atgtaaataa ggcaaattta tatatggata aatttaatct tatttgtgat gaaaataatt
      181 ttctaattga agaaattatt tttaaatcac aacttattta cattaaggca agtaagtgta
      241 tagaatcttc tgtaataatt aaagaaatag aaacgttgta tataaagata aaagaaatta
      301 acgattttaa ttgtaaaaaa atatattttt ttattattgg taaaatatat tttttgtttt
      361 tggatgatgc tctaattgct aaggtaaatt ttttaaaagc aagaaaatat gcagatttac
      421 taaaggatat gcaagtatct tcactatgta atataaagat aggtgagtgt gaatgttcgt
      481 ttgaaaatta tgaatgttcc atggaatatt tcaatgaagt aataagcaat aaaaagtata
      541 gaaatgtaaa tataattcaa aaatatagag catcaaacaa cataactaaa atattaataa
      601 agactagaaa ttatcctgtc gcaataaata atttagcaaa atctgaagta tatctagaaa
      661 aaattaaaaa tcatagctta aaagaagtag agaagtttga tttatttata agtttagcca
      721 tgtattatgc aaaaagtaat gaaaaatctt ttgaacaatc aatatgctat ttagatagag
      781 ctaaaaacat attaaatatg ataaaaaata tagaagaata tataatatat gatttagaaa
      841 taatttatca ccaggcaaag atatattata tctttggata ttatgacaat gccttaaata
      901 caaataaaaa actccttgaa aaatctaaaa atgctaaaaa ttataattat gtgaagttag
      961 catacaagag tatatatttg tgttttgaaa agattcaaaa ttatgaaatg agtatgaaat
     1021 actttaaaat gtattatgag ttaaaaatga tgtatataaa agctcgaaat agaaattata
     1081 tagattcctt aaattataga catgagtata tagaaaaaga aataaataac atgagaaaag
     1141 taaaacttga tttagataaa aaaaagtatg ttgaccattt gacaagtgca tataatagaa
     1201 cttttttgaa cgatttttta gaaaaacaag aagttagtga atattgcaca gcatttatga
     1261 tagatgtaga ttactttaaa gaatacaatg atacttatgg acattataat ggagatattg
     1321 ctcttaaaaa tataactata ataataaaaa aatatctaaa aaaagacatg cttatgataa
     1381 ggtatggtgg agaagaattt ctgatattat ctgtatgtaa agattataaa aaaagtaaaa
     1441 tatttggaag aaaattatgt aggattgtaa agaaattttt aaataataat ttgacagtta
     1501 gtattggcat tgatacatgt aaaaatagta gtatagatat aaaagaaata attgaaaatg
     1561 ctgataaagc tttatataaa gcaaaggaaa gtggtagaaa tagatgtttg cattatcatg
     1621 attttaagaa tttttaactg ttgaaatagg attggtgatt tacttgaaaa ggagtttaat
     1681 agaagattta aagagaaata taaacaagat agaatacgat atgttgacta aattctatct
     1741 aaatttgaca gatttaaact acatcttgga ttatcacaaa gaccttttaa atacaaaatt
     1801 agaagataat agtagagtct atactttata tttaattagt cttataaact atataaaaaa
     1861 agatagagaa gcatcataca actatttgat agagtcatta aagtatatta aaaaattgtc
     1921 attaaaagat gattcagata taatatctaa aatttatatt tatcttactt tatcagctat
     1981 ttctataaga aaatatgata aggtaaataa tttttatttc tcagcaaaaa aaattataag
     2041 gcaaaatcat ttaaatgagt tgttggtatt gttaaatatg agtttatgtg tggaaataag
     2101 tgctttatat cagaccaaaa cggatgtaat agctttagta gaagaagtgt ctttttttaa
     2161 aactttaaaa aataaagctc ttgtaagtag ggcaaattat atatttggga gagtttatct
     2221 atatttattt aataacttta taaaaagtat ggaatactta attgaatgtc taaaattggc
     2281 tcaagaaaat aatttatact ctatagaggc tttttgtaaa tgtataataa gtttgtgtta
     2341 tttagagagt ggaaataatg tagaagctat aaaatacata aataatgtat tagatgttgc
     2401 acacagtaat aactcaatat cagtaactga aagagtgtct ataaaaatag accttatatg
     2461 ggcatattta aaagaagata tgaataaaga agcagaagtt attttattaa agactataaa
     2521 attaatgaaa gttgttgaag gtgtatataa ggattattta tattcattta tatttttata
     2581 ttcagctgaa attgagttaa agaaagataa ttgtagtttt gaaaaggtaa acacctattt
     2641 aattttatca aaaaatatat ataaaagttt tggagatgtt tttatatata attctaaact
     2701 agattacata tataagttgt atggagattt atatataaaa tttggtaata tagatgaagg
     2761 tataagttgt tatataaaag gtttaaattt agttaaaaag agttcattga atcttagaaa
     2821 aatatctata tttatatggg ctaatagcaa atggatatga gctaaaaaaa gattttaaac
     2881 tggcaataga atattataaa agtatggatt ggtattctga taggtgggaa aaaaataatt
     2941 cctataaaac atctcaagca attcacaaac aatatgaact cagacaaaaa caagagagtt
     3001 taagattact agtggatgat aataagaaat tagaaaatga tattttaaaa gatggactta
     3061 ctaaactata taatagaaga tatttggaac agcagttgga tttatttaat ggaaccagag
     3121 acaaaaatat ggtcgcaata ctggtcgata tagattgttt taaaagttat aatgataatt
     3181 atggtcatct agcaggggat aaagttatag ttaaggtaac tgatattata agaagtgtat
     3241 ttatagatat taccgaacac ataatcagat atgggggaga agaaatttta atattagtag
     3301 attctataga atcaatcggt gttgaagaga ttgataatat agaattatat attaaaagag
     3361 ttttcaaact tcttgaattt gagggtattg aacatatata ttctaaaata aaaaactata
     3421 taactataag tgctggagta agtataaaaa ggtgtcgtag tagaaaagat gttgaaatat
     3481 taattgaaga tgcagataag aacctatata aatcaaaaaa agcaggtaga aatcaataca
     3541 ttataattta aaattgaggt gattttgttg cgaaaaaaaa taggaattat agcctctgat
     3601 attgaattaa aagaaagaat agaagaatta tatagagaag atgtagaaaa tggaactata
     3661 ataatcgaca tacttaacct agacttaatg gaaaatcaag gtcgtatact tgttgaaaaa
     3721 ggtgcccaag ctataattgg tagaggtgga ggttatagct tagtaataga tacagtaaat
     3781 gtacctgtta taccaatgaa tatgaagtca actgaccttt taagagcaat agaaatagca
     3841 aagaaatatt ctaaaaaagt agtattgatt cttggagaca atgaagtttc ttttgactat
     3901 gttggctgga gaaatgtcat aagtactgaa ataactgaag aatggtttga atctaagtat
     3961 gagataagaa gtaaagttgt aaaatatata gaccaaaaag atgaagttgt aatcgtagga
     4021 ggaggtttgg catgttcttt tgcaaggcaa tatggaattg atagtgtatt tgcaactgct
     4081 agtgatgaat ctataagaga agctgtagaa tattgtaaaa aattattaga tacattaggt
     4141 gaagaaaagt ttaataatga agtgttacga aatatacttg atggtataaa agatggagta
     4201 attgctatag atagtaatgg cagtataatt ttgtacaatg aaagtgcaaa aaacatgctt
     4261 aaagtagaga gaaaatgtgc acttaataaa tacatcttag atgtatttcc taaaatggag
     4321 tggatgttag attgtttaca tgaaaaagag gacgtagaag atagaaagat aagaaatata
     4381 aataatctaa ttgttaatac taggacaaca ttgataaaag ttgataatag tacttatgga
     4441 gttttaggaa taatacaaga tataaccaag ctacaaaact tagaaagaaa aataagattt
     4501 gacttgaatc aaaaaggtct ctatgctaga tatacttttg atgatttttt atttaaagat
     4561 aaattaacta aagaatttat tgaagaagct aaaaaaattg gaaaaagtga ttatacaact
     4621 ttactttatg gagaaagtgg gtctggtaaa gaaattatag ctcatagtat acacaacata
     4681 agtaaaagaa aagataggcc ttttgttgct ataaattgtg ctactatagc agaaaattta
     4741 cttgaaagtg aactttttgg ctatgaagaa ggtgcattta ctggtgctag aaagggtgga
     4801 aaaagaggac tatttgaact tgctcatgga gggactcttt ttttagatga aataaatagt
     4861 ctatccttta atattcaaac aaaactatta agagtaatag aagaacgtca aataatgaga
     4921 attggttctg attatataat acctctggat ataaggatta tagcagcaac gaatgaatct
     4981 ttaaccgaaa aaatagttat gggtacattt agagcagact tattttatag attgagtagt
     5041 ttagagataa atatacctcc tttaagagac agaagagaag atataatacc tttgtttaac
     5101 aattttgtaa atgaagtatt aaaggatgat ggtctcaatg gaataaacag tatagatgaa
     5161 aattttgttt tgactaaaga tgaaatggat aagctatata attatagctg gcctggaaat
     5221 gtaagagagt taaagacaat agctcaaaaa tatgttgtga caggtaaaat taaattgaga
     5281 caagatagag attttaaaac taaaaaatta cttcctaact ctgaagtaga caaatttaat
     5341 tctgaaacga cagcaagtgt agaagtccaa gatgaatcta taaatataag taagataaat
     5401 gatggtaaga taagtataga tataaaagaa gtaaataagt atgttgaaga aaaaataata
     5461 agtatgcttt ttgctcaggg actttctaaa aatgaagttg cacaagttct tggaataagc
     5521 aggacatctt tatggaagaa atacaataaa aatatttaaa tataattaca tataaattat
     5581 tttgaagtaa tgttaatttt ttaaaacaag tgttaagaaa aattaacatt acttttttgc
     5641 aaatttttat ttttttagac aggcatatat atttataaga aaaatagtta aaacacttaa
     5701 tttaataatg aaaaaaataa taattctaaa caaaatcaat tttacataaa gtgggattta
     5761 ttggcatagt aattgcttaa tatataatgt aagagtaaat acaattttag gggtgatggt
     5821 atgagtcaaa gtatggttgg taagcatgca atgtggccaa aagaaaatga tgttatattt
     5881 agcatatctg gcagagctca agcagcagag aaggcttttg gtatggataa tgtaataaat
     5941 gcaacaatag gcgctctaat ggatgattca ggaaaattga taacaatgaa aactgtatat
     6001 gaagaatata aagcattaga taactgtgag ataggtgcat atgcagctct tgaaggtcaa
     6061 ccagattact tagaagcagt taaaaaagta tttttcagag attacttacc agaaggacat
     6121 ataagagtat tagcttcacc aggaggaagt ggtgcaataa aattagctgt atggaactat
     6181 actaatgaag gagatgaggt attaacatct gattggttct ggagtccata tgtaagtata
     6241 gctgaagagg ctaatagaaa agtagttaat taccaacttt ttgatgaaaa tagaaggttt
     6301 aattttgagt cgtttaaaga aaaatttgta aatatagcag aaaaacaagg tagagtcttt
     6361 acaattataa acacaccagc gcataatcca actggatata gtgttgcaga tgatgagtgg
     6421 gataaaatat tagacttatc aaaagaagtc gcaaaagata aagacaaaaa gataatattt
     6481 ttcgtagatt cagcatatat tgactttgca ggtgatgatg atgtatgtag aaagtttttt
     6541 aagaaatttt ctaatttgcc tgaaaacgtt ctcgtattgg ttggattcag tatgtcaaaa
     6601 ggatttacag cttatggtat gagaatggga gctataattt gtatatcttc aagtgaagat
     6661 gtagctgaag agttccatta ctcatgtgtt cattcatgta gagcaaattg gtcaaattgt
     6721 aatagaagtg ctatggcagt attatctaac attgtaaatg accctaaaaa attcaaagaa
     6781 tatgaggatg aaaaagaaat atacaaaaac atgcttacta gaagagcaga tgtatttgta
     6841 aaagaagctg aaagagtagg attagaaatt cttccatata tagcaggttt ctttgtaagt
     6901 ataccatgtg ataatccaaa agaagtatgt gaagaattga ctaaacataa tttatttgca
     6961 gttccattaa aaatgggctt aagatttgca gtttgtgcag tttctgaaga taaatgtaag
     7021 aaagcaccaa gtattataaa agaagcactt gaaagtttag aagtaaaaat aaataattaa
     7081 tttcattgaa caaattgggt ggactatgaa tttaataatt gtagatggag ggaattttat
     7141 atgaaacaat tgatgacagg taatgaagca attgcacgtg gagcctatga ggctggtgtt
     7201 aaatatgcat ctgcatatcc agggactcca agtacagaaa tattagaaaa tatagcaaca
     7261 tataaagatg caatagttgc agagtgggct ccaaacgaaa aggtagcact tgaggctgct
     7321 atagggggtt caattgcagg agcaagaaca atggcttcta tgaaacatgt tggtttgaat
     7381 gttgcagctg atccaatatt tacttatgca tatacagggg taaatggtgg tatggtactt
     7441 attacagcag atgagccggg aatgcattca tctcaaaatg agcaagacaa taggatgtat
     7501 gcaaaatttg caaagatacc tttatttgaa ccatcaacta gtcaagaagc taaagatatg
     7561 ataaaagaag cttttgaagt tagtgaaaaa tacgatacac cagttttata tagagttaca
     7621 acaagacttt gtcattcaaa aggattagtg gagtgctatg atagagaaga agttgaaatt
     7681 aaagagtatg ttaaaaatgc taagaaaatg gtaactgttc cagcaaatgc tcaaataaga
     7741 aggggcgttg ttgaagaaag aatggaaatt cttaaaaaat tctcaaatga aactgattta
     7801 aactactatg aaataaatga tacaaaaata ggtgttatag catctggtat gtgttgtaat
     7861 tttgccaaag aagtttttgg aaaaaatgca tcatatatga aactaggatt tacaaatcca
     7921 ttaccttatg aaaaaataaa agaatttgca gaaaaagttg ataaaatata tgttatagaa
     7981 gaaaatgacc catttataga agaacaaata aaagcatatg gaatagattg tattggaaaa
     8041 gatgtaatac caccatatgg agaaatgaca ccagatgttt taagaaaagc tatatttgga
     8101 aaaacaaatg atactataga atacaaatct gaattagtaa caccaagacc accaagtttc
     8161 tgtgcaggat gtccacatag aggattcttc tatgaacttg gaaagagaaa aaatttaata
     8221 gttggtggag atataggttg ttatactctt ggatttgctc ctccatataa tggaatagat
     8281 tatgttgttt gtatgggttc tgcttttgga actgcacatg gagctcaaaa agttcttaat
     8341 atgaaggatg ataatgaaaa aagattggta ggtgtacttg gagactcaac attcttccat
     8401 acaggaataa atggactttt agatgttgtt tacaatagag gtaattctat ttcggttata
     8461 ttagataata gaataactgg aatgacaggt caccaagaaa acccaggttc aggatatact
     8521 ttacaaggtg ctaaaactaa agaagttgat atagaaggat tagttaaagc ttgtggaata
     8581 gaacatgtaa gagttataaa ccctaataat ttaaaagaag taaatgaagc actagattgg
     8641 gctttagcta tagaagatga accatcagta ataataacaa gatggccatg tgtacttaaa
     8701 aaattctcta aagaagatat agaagaattt aataatccat ttaagactaa gtgtaaagtt
     8761 gaccatgata aatgtatagg atgtaaatta tgtttgaaaa ctggatgtcc agctctatca
     8821 tttgataaag agaataagtt atcaaatata gatagaaatc agtgtgtagg atgtggagta
     8881 tgtgcacaag tttgtccaaa acaagcaata gttaaggagg aaaaataata tgactaagag
     8941 tatacttttg gtaggtgtag gaggtcaagg aactatactt gctagtaaat tattaactat
     9001 gggattgatg gaagctggat atgatgtaaa gatgagtgaa atacatggaa tgagtcaaag
     9061 aggaggttca gtttcctcac aagttagata tggagattgt gttcattctc cagttataga
     9121 aattggtgga gcagatatat tagtatcttt tgaaaaaatg gaagctttaa gatggttcaa
     9181 ttatttaaaa ccagaaggaa aagctttagt gaataatcat aggatagatt ctatgactgt
     9241 tttaatcgga ggagctgaat atcaagaaag tgatatagat gcagagttaa gtagattaaa
     9301 tgcaaaagtt ataaatgcag ctgataaagc tgaagaacta ggaaattcaa aaataatgaa
     9361 tgttatttta ttaggatgtc tagttaaatc tatgcaatta gagtctattg attgggaaaa
     9421 aattataagt gaaaatgtta aaccaaaatt tgttgaatta aacataaaag ctttccatga
     9481 aggtatggaa atggttagca aatagtttat aatagatata aatagactat agaaattaag
     9541 gtgaaaggaa gaaaattatg acttacagaa tattagccat aaatccaggt tctacttcta
     9601 caaaaatagc agtatatgat ggagaagaac aaattcttgt gaagacgata gaccatccag
     9661 ctgaagagat tgcaaaatat aatactatac aagaccagtt tgaaatgcgt aaggaagcag
     9721 ttttgaatat tcttaaagaa aatagtatag acttaaaatc tcttagtgca atagtaggaa
     9781 gaggtggagt tttaccacca gtaaaatcag gagcatattt agtaaatgaa gaaatgattg
     9841 atgtactaag acatagacca gtacttgaac acgcttccaa tttaggtgct gttgtggcac
     9901 atgcaatatc agaacctctt ggaatcaact catatattta tgattctgtt gcagtagatg
     9961 agcttataga tgtagcgaga atatctggac tttgtggaat ggatagatca agtgcagggc
    10021 atgcattaaa tactagagca atggctttaa aatatgctaa ggataaagga aaagattata
    10081 agagcttaaa cttaatagta gctcacattg gtggaggagt aagtatttat cttcatgaaa
    10141 aaggaagaat ggttgatatg ctatctgatg atgaaggacc attttctcca gaaaggtcag
    10201 gaagagtacc tgctacaaaa ttagtggctg cctgttattc aggtcaatat tcagaaagag
    10261 aaatgactaa aaagataaga ggtaaaggtg gtatagtttc atacctaaat actgtagatg
    10321 ctagagaagt tgaaaaaatg atagcagaag gaaatgaaga agcaaaaatt atttatgaag
    10381 caatggctta tcagttagca aaaggtattg gagagttagc aactgtagta gatggaaagg
    10441 tagatgctat aattataaca ggtggaattg catattctga aatgtttact tcaatggtta
    10501 aaaagaaagt tgagtttata gcaccagtag aaattatggc aggagaaaat gagttggaat
    10561 cacttgcttt tggaacttta agagtactaa atggagaaga agaagctaga atttatagtg
    10621 aaaattaata agaaaaacta aaaatgttta tatactaaaa aagtggtata tccgtttttt
    10681 atacacgaaa gcttagagta taggtgaaaa tgtataaaat ttggattgcc actttttata
    10741 attaaaattt tatcttaacc taataaataa ttctgtaaaa tttcgtattt atatatatat
    10801 tttattggta tttattgtta ttattattat gaggagtgat ttttgatggg aaaaataagt
    10861 ttaaaaagta tgataagatt attttcaggt ttttttatat ttgcaatttc ttctgtgctt
    10921 atgattaatg cacatgtagg tcttatgcct tgggatgtac ttcaccaagg tttatctata
    10981 aagctaggca taactatagg tcaggcttct ataatggtag gagttgtaat tgttatattg
    11041 gatgcagttt ttggtgaaaa tataggatgg ggtactttac tcaacatgac atttatagga
    11101 atttttattg atttagtaat attttctgga gtgatacctc atgcaagcaa cacttatata
    11161 ggtgtgttta tggttgttat aggaattatt ttggcagcta tagcaagttt tctatactta
    11221 ggtgtttgtt taggaagtgg accgagagat gggcttatga ttgctcttca gaaaaaaact
    11281 aataaatctg tacgtcttgt tagaacaatt cttgaaatac tagcattagt tgttgggtgg
    11341 ttattaggag gttctgtagg tattggtacg cttgtaagtg ctttaggttt aggatatgta
    11401 ttacaaatag tgtttagaat atttaaattt gacacaaaat tgttgaaaca tagatttatt
    11461 atagatgata taagagaatg gaaggaaaag aaaagcaatg aacataaatg taaatcaagt
    11521 attgtaataa aaaatgagca gaattaatta tgctaaaata tatttgtagt ttatctaatt
    11581 tatagtaaag tcaaggagat tttgaaatgg aattatggga tttatataat gctgatggaa
    11641 ttaaaacagg aaatgttata aaaagaggta attctattga agaaggttat taccatttgg
    11701 cagttgaagt gtggatttta aatagtaact ctcaaatttt aattcagaaa aggtctaaaa
    11761 gtaaaaagac attgccaaat atgtggggaa tgacaactgg atgtattgtt tctggggaag
    11821 acagtttaga aggagcaatt cgcgaagcaa aggaagaaat agggatagat ataactaaag
    11881 atgagatgaa agtttttaga agtatgattc atgaagatac tctttgggat gtatacttag
    11941 ttaaaaaaga atatgatata tccaaagcta ttttacagga ggaagaagta agtgatataa
    12001 agtgggtaag cacagatgaa ataagacaat tactaaaaga gggtttgttt tttgaatatc
    12061 cagagatata cgaattgcta tatgagatag ataataataa attaaatata tgatgtagag
    12121 cagatttcag tataagtaag attatttaaa atgtatcttc tctaaatttt aatattgttt
    12181 tatgaaaatt aataagttat ttttagtatt tttctacttg ttttttgaga ttaaaataaa
    12241 aagggcagtc attataaagt cataaaaatt tggggaggat ttaaactatg gaagataaca
    12301 aaggattggc acgtgacagt tcaaaatata aaattgtttc tatatctgtc gtagtaatta
    12361 ttctaattac attttggtat atgttaaata tggtgctttt aacatttatt atgactttta
    12421 ttttctataa cttgcttgta gccacaagaa aacgaattaa gaagttttct tctttaaata
    12481 ttccagattc attaataatt atagtgttat atgcactatt tgctatattg ttagtattga
    12541 ttagctatgc agttgtacca ataattatag ttcaacttac agagttgagt agggttttta
    12601 gtgattttga tgtaaatcaa tttgctcaat ctttaggacc aaaattgtat cctattgtct
    12661 caaagctgga ttttaataaa tatatttcac aagcagggct acttatagca tcaacagcaa
    12721 caaaagttgg aagttttgga gtaaatatat tattggcatt tttactgagt ttactccttt
    12781 tacttgaaaa aaatgaaatt aaaaattttg gagataaact ttctgatagt aagatttctt
    12841 ttatttataa ttcgcttgta ttttttggaa agagctttgt taaaaatttt ggtgaagtaa
    12901 tgaaagtaca agttatgatt gcctttataa attctgttgt ttctatgatt ttcttaggtt
    12961 tcatgggatt ccctcaaata tgggctttag gatttatgat ttttgtgttg ggtcttattc
    13021 cagtagcagg agttatagtg tctttgattc cattaactgt aatagcattt aatacaggag
    13081 gaataacaaa ggtttttgga gttttactta tgatttgtat agtgcatgca gtagaaacat
    13141 acatattaaa ccctaagttg atgtcgaata gaactaaact tccagtttgt tttgttttta
    13201 ttattttact tgtaggagaa cattatttag gtgtatgggg attactaatt ggagttccaa
    13261 tatttatgtt ccttatggat attctgggtg ttaaatttac ttctaggtaa tatattaaaa
    13321 taataattta tctttatata taaattataa attgttttaa aaaagaacct ataaatagac
    13381 atcattaata aagacatcta tttgataggt tcaattttgc tttgtataaa tactataaaa
    13441 ctaaataaaa agaaatttat ttcatttcaa aagtttcaca acttgcattt ctttcattta
    13501 tatgaacttt ttcagctttg caaagttcat ttttattgta tttacatttt acagcttcac
    13561 aatgaatgtt atctgtatca acttggttag tttcattaga acgactagtt ataccagatg
    13621 tatatttgtc ttcaaaagaa gcacaataag tattttctgt agtagttgct tgcattccat
    13681 ctacttttat actaccagca taacaatatc cactagtatt ataagcacaa ttagtagcag
    13741 cacaatttaa attattattt ttcataaata acctcctaag ttttatttaa acttattatg
    13801 tgaagttatc taaattttat acacttattt acaattacat ttatcacatt taacaactac
    13861 atctttggaa gtttcccttc ctaaattttt attaacatga tacacatcaa cattttcagt
    13921 tgatccacat atagaacatt taaaattgtt ttctagtagg gttctttctt taaataactt
    13981 ccaatacata gaattgatgt aatcattata atttaccttg aggcatgtct caagaaagtt
    14041 tatgtataga ttgtctaaat ctcctgtatt taattctgta tattctggtt caaaatattc
    14101 acaacatcct ctaaaatcgg gctttgtatc tgaaataaca catctaatat catatctata
    14161 gtctaggcta ggccccttaa atgaaagatg tgtgcaaaaa gaacaagatg tttctggtag
    14221 atttttttca agttcatgta atttattatt cataagtatt cctccaaaaa atttatttta
    14281 agtatataaa cgaatagatt attttaaaac ttttatttat ttatattaga gatttaggat
    14341 aaatattcca ataaggagta aatacttaag tatttattac tgtattaaat aaaaaagagg
    14401 caaatctaac ttgagattgc ctactttttt atcatttttt aataaaattt attctaagta
    14461 ttattttatt tagatttagt cagccaactt agtatctgct ttgcttttta actttctacc
    14521 agagcgtaca gttaaaatag catataatac agtaaatatg gcagctaaaa catatgatat
    14581 atttatgttt aagttaaatc caatttgagc attaaatata taactgaata taacaaagga
    14641 ataaaacata ccaggaatta agcttataat ataattcttt tcttttatta taaggtaaac
    14701 tgtaatcatt gcaaatgcaa atatggcaat agtttgatta ctccaagaga agtatctcca
    14761 caatatatta aatccagaag cactaagctt tgcaaatata aggatggcta tgacaggaat
    14821 aaatatacag attgacaata taacacgatg ttttttttcc ttttggtcat aatgaaagta
    14881 atctgcaagc attaatctta aagatcttaa agctgtatct ccagaagtta ttggtaaaac
    14941 aattacacct attatagcta tgataccacc aatagaacca agtaaatctc ttgcaaccaa
    15001 acctattaca tcaggagaac ctactaattc cttaggtatt cctttattgt aaatacccat
    15061 agcagctgca gcccaaatca ttgcaattaa accttctaaa atcatcatat tataaaaagt
    15121 agtttttcct tctttttcat ttgaaactga tcttgctata agagtagctt gagttgagtg
    15181 gaatccagaa acaataccac aagcaactgt aacaaagaat gtaggaataa gaggaatacc
    15241 atcaggatga atacctttcc aatttgcaag acttaaatta gctaaatcat aaccttgtgt
    15301 aaagattcca attccaacac cagcagcaga taaaagtaga atagctccaa atattggata
    15361 tacctttcct atgattttat caattggaaa tagtgttgca cacaagtaat atagaaagat
    15421 aagtccataa actatccaga caactggatt atttatagtc gatttcatat ttaaaatttg
    15481 agtaacaact aaatccccag gtgtataaat aaaaactgca ccaactaaaa gcattaaaag
    15541 acaaaggaat atattataga cttgaaatac tttattacct aaaaatctac tgataagaga
    15601 aggcatttgt gctccttttt ctcttatact aatcattcca ctcatatagt catgcaaagc
    15661 tcctccaaat acacagccaa taggtattag tatgaatgct attggaccaa acaatatacc
    15721 ttggataggt cctagaattg gtcctgtacc agctatatta agcaactcaa ttagactatt
    15781 tttccatttt ttcattggga catagtctac tccatcactt tgagcaagtg caggtgtttt
    15841 tctgtcatct gggccaaata ctttttcaca ataagcaccg tataaagcac ctcctattag
    15901 taatatagcc aagccgatta agaatgttgt cataatatca ccccatttat aaaatttttt
    15961 ggtctttgta tacatattat ctaaatgtgt agatttatat tgaatttaat tctggacaac
    16021 ctacaaattc tataaagtat tacaactaat ataatttcag aaataaatac atatacataa
    16081 ccatgtactt agtatacagg tatttggtaa tattttcaat gttatagcaa taaagaatat
    16141 agacaaaatt ctgataatat tttacaaaaa aattaaaaaa tataaaaatt agtttaaata
    16201 gatggagaaa aaaggaaaat caattgagaa tataaatgtc tatatcttaa caatattaag
    16261 aggtttaaaa ttcttgaaga aagaatagtc aagtgatata atgagtttac aggtgtatat
    16321 acactagtac atatagaaag cggtgcaatt atggataaag atttaacata tcaaatcaaa
    16381 gagctaaaga aagaaaaaaa tgcaataata cttgcccatt tttatcaacc accagaaata
    16441 caagaattag cagatgcagt tggtgactca tattatttaa gtgagattgc tagagattgt
    16501 aaagaagaag tggttgtatt ttgtggagta aggtttatgg gagaaagtgc taagatatta
    16561 tcacctgaaa aaactgtatt gatgccagta agtaacgctg ggtgtgctat ggcagatatg
    16621 gtagatgaag aaggcgtgat aaaattaaaa caacagtatc caaatgcttt ggttgtatgt
    16681 tatataaact ctacagctaa agttaaggca cattgtgatg tttctgttac atcttctagt
    16741 gcaattaaaa tactagaaaa catagataat aaagaaatta tattccttcc agataaaaat
    16801 ttaggaggat atattgcaga acaatttcca gataaaaact ttatattttg ggacggatat
    16861 tgtaaatacc ataacaatat aagagctgaa gaaataattg agttaaaaga taagtataaa
    16921 aatgcagaag tattagtaca tccagaatgt aaaaaagaaa taagagattt aggagattat
    16981 gtaggtagta catcaggaat aataaaatat gctactaata gtaaaaacaa agactttata
    17041 atagcaactg aagaaggtat attacatgaa ttaaagaaaa ataatcctaa taaaaacttt
    17101 tatattcctg gtggaaaaat actatgtaca gatatgaaaa agacaacatt agaaaattta
    17161 tatagtacac ttaaaaatat ggaaaatgaa gttattgtag aagatgaaat catggaaaag
    17221 gctttaaatt cactgcttaa catgcacaaa ttagcagaag gatagaggga tagacttatg
    17281 aatttagaac aagatgtttt aatagttgga agtggagttt caggattata ctgtgcatta
    17341 aatttagata aaagtttaaa tgtgctagta gtatcgaaat caacaattga aaacaacaat
    17401 acatatcttg cacaaggtgg aatttcaact gctagaaata ttgatgatat agaatccttt
    17461 gtagaagata caatgaaggc tgggcagtat aaaaatagag ttgaagcagt tcaagtactt
    17521 gcagatgaat ctatagaaaa tgtagggcag atagtagaat atggtatgcc tttagacaaa
    17581 gaaaatggag agatagatta cacaagagaa ggagcacata gtgtaaatcg aatagttcat
    17641 agtaaggata atactggaga agttgttttt aaaactttat taaaagaagc taaaactaga
    17701 gaaaatataa ctcttataga agatgcctat cttttagata tattaaaaga tggcaataaa
    17761 tgtataggag ctagaatttt taaatctaaa aaagagattc atgttttttc aaaaatagta
    17821 gttttagcta caggtggaat tggtgggctt tttaaaaatt ctactaatca aagacatctt
    17881 acaggtgatg gaattgcaat agctttaaga aataatataa aaatagaaaa tttagaatat
    17941 attcaaatac atcctacagc cttttatgaa gaaaataatg aaggtaggag aatgcttata
    18001 tctgaatcat taagaggaga gggagctaaa cttttaaata aaaataaaga aagatttgtg
    18061 gatgaacttc ttcctagaga tgtagtttca aaagctattt ttgagcaaat ggaaaaagat
    18121 aaattacctt atgtgtattt agatgcaaca catttagata gtgaatattt aattaataga
    18181 ttttctttta tttataatga atgtttagct agaggaacag acataacgaa agaatgtata
    18241 aaggtatctc ctgcacaaca ttattttatg ggcgggatac atgtagattt ggattcaaaa
    18301 acatctatgg aaaatttata tgcagttggt gaaattagtt gtacaggtgt tcatggagcc
    18361 aatagattgg caagtaattc attacttgaa gggcttgtat tttcaaaaag agcagctaaa
    18421 aatataaact cagttataga taatgtaaaa gttaagttta tagatgtacc agatatggat
    18481 atagatatag aacaggtaaa aaaagaaaat aaaatattag tcataaaaga aattgaaaga
    18541 acaagtgagg attttggcga tgaattattt gattattgat agaatgatac aggatgcttt
    18601 gatagaagat gttccaagtg aagatataac aactaattct attgtggatg aaaattctaa
    18661 atctactgta gatttaattt gtaaacaaga tggtataata gctggcttag gtgtatttaa
    18721 gagagttttt gagatacttg gtgatgtaga tgtaaaatta tataaaaatg atggtgatag
    18781 agtaaagaat cgtgaaaaga tagctgtatt aacaggtagt actagaaact tacttgtagg
    18841 agagcgtgta gcattgaatt atttacagag aatgagtggt atagctacta ttacaagtag
    18901 gtatgttgaa aagcttgaag gtacaaatac aaaattatta gatagtagaa aaacgatacc
    18961 taatcttaga atattagata agtattctgt taaagtaggt ggaggatgca atcatagatt
    19021 taatttatct gatggaatat tgcttaaaga caaccatatt ggggctgctg gtagtgttaa
    19081 gaaggcagtt gaattagcta gaaaaaatac ttcttttgtt agaaagatag aagtggaagt
    19141 ggaaacttta gacatggtaa aagaagcaat agaagctaat gcagatataa taatgttgga
    19201 taatatgagt ttggaaatgg caaaacaagc agtagatatt ataaatggaa gagccattgt
    19261 agaattttcc ggaaatgtaa atttagatac tatagaagat ataggaaaaa ttggtgtgga
    19321 tgttgtttct gtaggagcgc ttacacattc tgttaaagct ctcgatataa gtatgaagaa
    19381 tttgagaaat atataaattt tatattgaaa taaacttaat taaaatagac tccttaaatt
    19441 tgttttataa atttaaggag tctatttttt attcattaaa attaataaaa atagaaaaaa
    19501 atcaatcaat aatttaataa aaatagataa atactatata cacttttaat aatatagtta
    19561 atatctattt tacagataaa taagaataaa cttataatat aattgtatct gtaaaatttt
    19621 gtaaaatata aaaagtatag ggggaataaa aatgaaagta gcttatattt ttagtagtac
    19681 taatacacat aaaattctag acaaaatgat aataccacaa cttgaacaag gaactcatgg
    19741 agtagacgtt ctagggatgt ttttctttat ggataatacc ttatttttat ctaaaggtaa
    19801 tcctgtaggc gagagactaa gtaaattaca tgaaaaaact gatatgataa taatggcttg
    19861 tgaccaatgt gctatagaaa gaggtataga gaataatttg gttgatggag ctacaattgg
    19921 atgtttccca aacctatatg cttgtttagg cagtgcaggt gtggaccaag ttataacgtt
    19981 ataaattagt ataggataca atccatagaa aaatttattt tctgtggatt attttaagta
    20041 aaagataaat tgtttaaagt aggggattaa caaatgtcta aaattttagt tgaatttata
    20101 aacacttgac catcttgtga tgaacatgga gtgtttataa aaaaaatgtc agaaaaatat
    20161 gctgataaat tggaaataaa attatatcaa gcagggaaag atttttcgta tataaaaaaa
    20221 tatgggatta taacaaaagg aactttaata ataaatcaga aaaagaaata tgatagatta
    20281 aataaagata caattgaaag ggcaatagta gaagctataa acaataatta aaagtttaaa
    20341 aattaggagt atcaaaaatg aatatattag aaattgtttt aaatggagtt aaagatacta
    20401 tagtagcttc tatagttaca ctgaatggag catcaggatg gcttatgata agttttatat
    20461 tggcaggtgt acttcacaat gttttaactc caagaaggtt tcaaaaacaa cttggaaata
    20521 agaaattttc ttctttaatt aaaagtacta tctcaggaat gtttttacca atatgtagtt
    20581 gtggagtaat accacttgga ataagtatgt attattcagg agcatattta ggaccagtac
    20641 ttgcttttat gacatcaact ccaataataa atccaatagc tgtagttctt tgtctaggtc
    20701 ttttaggtcc taaagttaca atcatttatc taatagctgg ttttttagta ccatttttag
    20761 taggattttt aggaaattgt tttggaaaag aagaattatc acttcataat gattcagagg
    20821 aagaagtaat agaattagaa gaagaaaaaa gaagttttat gggaaatatg attgatggtc
    20881 ttaaatggtc ttttggtgaa ttagccctta ctattagtaa atatgtagta atgggtatgg
    20941 tttttgcagg atttataaca actatttttc caaatagtat aattcaaaaa tatttaggaa
    21001 atccaggttt gctatctctt ggaagtatag caatacttgc atgtgttatg tatgtatgtg
    21061 ctgtaggaca tataccgttt atagcagccc ttgttgcaag tggagcatct cctggtatag
    21121 cgataacatt tctaatagct ggagcagcta caaatctacc agaacttata agtatgttta
    21181 aacttattgg aaaaagaact gttattatat attctgtaac tttgacactg tcttcaataa
    21241 taattggata tataacaaat ttacttttag tgaataataa ctcagtaaat ttagatgcaa
    21301 caaattcttc aatacagttg gcaaataaac taatgttaaa tatacctgat agttttgagt
    21361 atgtatgtac tattttaatt attttatttg catttaaatc aataatacca aagatgaaag
    21421 aggtcatagg aaatcatgct taaaaagata gttataatat cctgtatagt agttgtgtta
    21481 atcattttat caaaaatagt tgatgataac atcaaagaag atgcttctat tccaaatgtg
    21541 aataaagaaa cattagaata ttttagaaaa aattataaag aagacataat aacttgtgct
    21601 gaagaagact taaataatga tggaaaaaaa gatttagtag ttatatataa aaaaagcaat
    21661 aattcaaatg aaatggtagt cgtagtttct gataaaaata gccactatat aacaaagcca
    21721 ataccagcac ctatagagaa tcaaacgata acctttaaaa atattgatga taaagcacca
    21781 atagaagtaa ttgtatcagg gtctaaaaat ggtaatgtag gatatgctat ttacagagta
    21841 gagggtaaaa aatttgttga tttatttggc gaagatatgg acaaatgttg ctaaaaataa
    21901 aaataataca taaaggtggg aaatttatat gaaattatct aagaagattc aagcattaat
    21961 cttatttggt gtaatgggta catctatatt aacaggatgt agtagtaaac caaaagaaaa
    22021 agaagaagca aaaactggaa aagatgatta tacaataaca gtaggttatt ataattgtga
    22081 ccatatgact gcgggaccag tagcagaatc ggcaggaata tataaagatt taggtcttaa
    22141 tgtaaaaact gttggaaatg gtaaagttcc tgaagctatg gcagcaggaa aaatggatgc
    22201 aggttacata ggaactaagg ggttagttgg agcaatacct aagggttcgc caataacaat
    22261 agcagctaac aaccatactg gtggttctga atatttaata gtttcaaaag atataaaaga
    22321 acctaaagat ttaataggta aaaaaattgc aacagatatg agtgatttct tatggacaag
    22381 tgattatggt ccagaaacag gtctaccaac tgacccatca aaatatgaag ttgtaaatat
    22441 ggattcagac aaagataaat atttagctct taaaactggg aaaattcaag cttttacatc
    22501 ttgtgaccct tggggttctg tagctgaaaa tgacggtgca ggaaaaataa tagcatctac
    22561 tcaatataaa gaaaaagcta atggcaaaga atataactgt tgttcattct cattaaacaa
    22621 aaactttata aaagaacatc cagatttagc aaaaaaattg gttctagcac atacaaaagc
    22681 aatagaatat atatatacaa atccagctga agcagctaaa atatttgcaa aatattataa
    22741 tgtagaagaa gaagttgcac ttagaactat ctataagaag actgttggag aaggtagaac
    22801 tcttacttgg aaagttacag gtgaagaata taaaaataat ttacaaatgt ataaagattt
    22861 aaaggcttta gatgatatac ctaaatatga agatacgatt gatacaagct tattggactc
    22921 ttgtggagct gatgattttg acaaatttat aaaagaaaaa gttaacccag agtttcctga
    22981 aggtatgagt tatgaagact ggaaagcaaa ggtattataa ataaatggag aatatgaaaa
    23041 atataataat actttttatc attattttgg gaagtatatt tataatattt tttaattttg
    23101 aagataaaga agaaataaaa ggaaaattaa gaataggggt atctgatgat acctctggtt
    23161 ttgtaataaa ttatatggta aatagagact actttgaaaa tataaaagta agtgatataa
    23221 tggatatttt tacaattaat gattgctgag caagtacaat gcagtgggct ctgagttcag
    23281 aggaaataga tatagcaatc atatgtaaag atgctgcaaa acaatatgta aatgtaaata
    23341 gtggatttga aattgttggt actgtagttc aaaattctga tatatttcta ttaagtgata
    23401 ataatcctga aaagatagga gtcattcaaa atagagatta ccagtcagaa ttggttaaaa
    23461 aatactacaa gaatgtggaa gtagctccct tattagggac tgcactagca tatggattag
    23521 aaagtaattt agtagatggt gttgtaatag attgcataaa gtcaattggg ttagagggta
    23581 aaagaaaatc tacaactaaa ttaggagatt atgataccta tgtattagtt gtaaataaag
    23641 agtttaaaaa aagtaaagca tatagtgatt ttataacact ttacaataaa agtgttgatg
    23701 ctttaaagaa taaagctaca tttaagaaag tattaaaaga gtacaggaat ataagaaata
    23761 tagatatgga ggaaataagc aattggaaat taaaatttct aaagataaaa gcgatagatt
    23821 aactaagaaa aaaactgatg gtagtatgga tattttatat aaaataatac tattagttat
    23881 tatattttta gtctggcatt ttgcagcaaa agatataggg agttctttgt tacttccaat
    23941 gccagtagat gtaataaaag gtttcttttt ctgtgtgaca gatgcagaga cagtcactaa
    24001 tctttttata acattgcaga gagtacttaa aggttttatg tatgcattat tgtttgggct
    24061 tccaatagga tttattatgg gattttctaa aacttttgaa agggttttat ctccagtagt
    24121 agattctgta aggcaagttc caataatggc atgggttcct cttactatag tatggtttgg
    24181 tataggagat ggtccaacaa tatttttaat agctttttca ggggttttcc ctataatttt
    24241 aaatactatt caaggagtaa gagcaatatc aaaggattat tataatgcag ctagaagtat
    24301 gggagcatcg ccaattgtga tatttacaaa tgtaattgtt cccgcatctc ttccagatat
    24361 actaactggt tcaaggattg ctataagtac aggatggatg tctgttatct gagccgagtt
    24421 tatagctacg agtgccggtc tcggttactc aatggtacaa gctcaaacat taatgcatac
    24481 agatgtatta attgggctta tgatatttgc agctttaata ggatttataa tagatagggt
    24541 attaaagttt ataaataaaa tcctttgtag atggaggttt gcagattaat gaagttaagt
    24601 gtaagagata taaataagac atttgtaaat aatagagtac atacaaaggt tttagaagat
    24661 ataagtatgg atatagatga tggacaattt gtctgtcttt taggaccatc aggttgtgga
    24721 aaaacaactt tacttactat aataggtggg tttcaaaaat cagaacgtgg agatgttttt
    24781 ataaatgata aaagagttaa aaaaccgggc atagatagag cttttatatt tcaaaattat
    24841 gctttatttc cttggaaaac tataagaggt aatgttctat ttcctatgaa acagcagaaa
    24901 ataccaaaag aaaaaagaga agaaatgcta gaagaacttt tggttatgtc agacttgaaa
    24961 ggaaaagaaa aactgtttcc gcatcaatta tctggaggaa tgaaacaaag agtggccatg
    25021 ataagagctt tagcatgtaa tccagaggta ttactaatgg atgaaccatt aggagctgta
    25081 gattttcaaa tgagacaaaa tttacaggaa gagctagaac gaatatggat aaagaagaaa
    25141 ataactgcat taatggtaac tcatgatgtt gatgaagctg tatatatgag tgatagagtt
    25201 atagtaatgt cgagagataa aggaagaatt attgatgata taaatataga tattccaaga
    25261 cctagaatta gaggaagcca aaaatatgaa gagtacaaaa acaaattaac tgatacttta
    25321 tcaaagtgtt atgaagttta aagaggagta gacatgagtc aaatagaaaa atacaatagt
    25381 ttggataata cacaacagaa aataagaatc tctattatgg atatgataat agataaaggt
    25441 tctagtgtta cacttcaaga agtgactgaa tatgtaagta aaaagttgaa tatagaaaaa
    25501 gaatatatag aaaggacact acaatatttt atttataaaa atataatggt tgttgatggg
    25561 aatagtataa attttatata tcctgtatca gcattgccaa caaatcataa agtaactttg
    25621 agagataata gaagtttcag tgcaatgtgt gctatagatg ctataggaac ctcttgtaca
    25681 ttcaaccaag atataaaaat aaactctatt tgtagtgcta caggtaagga aatagaagta
    25741 attataaaaa atgaaaagat tgagtatgtg aataatccaa acttaagagt gcttcatata
    25801 aatcttgata agcatctaaa ttgggctgct agttgctgaa atataatgaa tttcttctgg
    25861 acgaagagtg attttgatgc ttggacaaac gaagcgggat tatctgatga tgaagatata
    25921 tattgtttag atattaatga ggcaatagta gaatcatata aaatatttaa actcaaacaa
    25981 aaggtactat catagatata catatatgaa aaactaaaaa tgacctttag ataaaaatga
    26041 taggtaatat ccatactata taaattagta tagagtaggg atgtgaaaag tatttaaatc
    26101 taaaggtata tttttagttt ttttagatga ttagatagcc ttattaagta tatatttatt
    26161 tataaccttt gcaactccat tttcattatt agtatcagta acataatctg caatttcctt
    26221 tatactatta cgtgcattcc ccatagccac accaagacct gcatacttta tcatatgttg
    26281 gtcattttct tcatcgccaa cagcaatagc attttctata ggtatattta gattcgtaca
    26341 taatgtttta agagcagttc ctttattagt agttttacct aaaaattcta gataaaaagg
    26401 agcagacctt actatagtat atttattgaa taaattttta ggtagttgct gtatgatttc
    26461 ttccaatctt tctggttcat caactatcat aattttacag attttaatag attcatcaat
    26521 tttatctttt tgtatatatt tgacttctat attatttaat ttcgcttcat gaagtgtata
    26581 tttgccaggc gtttcatttg gtgtaattaa atttgtcatt gtatttatgt gtattttagt
    26641 cttatatttt ttacaaacgt tattatatat aaaatcaaaa tcatctaaac tcatttctac
    26701 atcatgtatg attttctcac ttttaacttc ttgaataatt gctccattaa agcatataac
    26761 ataatcacct tcatctgtta aatttagttg atttagaagt tcttttacac caggaagagg
    26821 tctgcctgtt gttataacta cttttgcccc agcccttttt gcctcctgaa tggactcaaa
    26881 gacttcagga gtaatccttt tttgtgtatt taatattgtt ccatcaatgt ctagtgcgat
    26941 taatttgtac atatcaacct ccaataaatt atatactcaa atttcctgtg aaaacgttga
    27001 cacagctagt gttgaacaaa gtttcagtgg gaaatatggt ttgttttgta tactattata
    27061 tcaagagata agtaatttag aaagtttaat aatattaatt ttttgataaa aatattactt
    27121 atgtgagatg agaatgtaaa aactttgtaa tcaataattc caaaattctt aattacaaat
    27181 aatggtatcg tttgcattgt taatataaat aaatgtatat tatatagtta gaagtttgaa
    27241 taaatagact gatggtaatt tgttatatta gttagttttg tgttataaaa tattgtaatt
    27301 ccattaatca tatgttatta ttcaacatga ttataatata aagaatattt gatatatata
    27361 ataatttata aaagagtatt acatagtaca tataaatcta tatttataaa tacaataaag
    27421 taaagttgta aaggagtgct ttaaatgaag ttttcagagt ttaagtatga aagacccaat
    27481 tataatagta tgaaaaaaga gtttttgagt tgtgtagaag atataaataa ttctcgtaat
    27541 tataaagagc aacaaaaaaa tatacataag attaatttat taagaaataa aatagaaacg
    27601 ttgtcaaata tagcttcgat aagatatagt acagacacat ttaataaatt ctacaaagaa
    27661 gaaaaaaatt attgggatga atatatgcct ttgtatgaag aattaaattc atatttttat
    27721 aatgccattg taaattcaaa atttaaatat gatttgataa aagagtttgg agagcaattt
    27781 ttcacaatag tagagtattc tttaaaaagt ttttccaaag aaataataag tgaattgcag
    27841 gaagaaaata aattgtgttc agagtatact agacttttag cttcagctga gataatgttt
    27901 gatgggaaaa taagaaattt atctggtatg gggaaattca tgtattctaa gaatagaaaa
    27961 acaagagaat tagcaaacaa agcatattat aatttctttg aagaaaatga aactaaattt
    28021 gatgatatat ttgataaatt ggttaaactt agagataaga tgtctaaaaa attaggtttt
    28081 gaagattttg tagagcttgg gtacattaga atgatgagaa gtgactacag agaatatatg
    28141 ataaaaaatg ttagaaaaca agtactaaaa tatgttgttc caatggcaaa tgagctttat
    28201 gaaaaacaag ctaaaagaat tggtctagaa tatttaagtt acatagatga aggcgtggaa
    28261 tttttaacag gaaatgcaag tttaaaggga gattcaagat acataattaa aaatggtaaa
    28321 aggatgtatt ctgaactgtc aaaagaaaca aatgaatttt ttgattttat gttagaaaat
    28381 gaacttatgg atttagaaac gaagaaagga aaaggagctg gagggtattg tacttatata
    28441 ccagattata agtcaccatt cattttttct aattttaatc aaactgctga tgatatagat
    28501 gtattaacac atgaggcagg acatgcattt cagttgtata tgtctagatg gattgatatg
    28561 ccagaaatta attttccaac gcttgatagt tgtgaaatcc attcaatgag tatggaattt
    28621 ataacatggc cgtggatgga tttatttttt aaagaagata cagataagta caaattcact
    28681 cacttatcat cttcaattaa atttatacct tatggagtaa ttgttgatga atttcaacac
    28741 tatatttaca aaaatcctaa tgtagataag tcaaaacgaa aagaaatctg gagattttta
    28801 gagaaaaaat acttacctca tagaaaatat ggtgataatt cttttttaga gagaggttgt
    28861 tggtggttta aacaaggaca tatatttaaa aatccgtttt attatataga ttatgtatta
    28921 gctcaaattt gtgcgttaca attttggaaa aaaatgattc aagatagaga tgcaggatgg
    28981 aaagattata taaatatctg taaagttgga ggaacaaaat catttttaga tattgtgagt
    29041 atgggaaatt tgtattctcc atttgatgat ggctgtattg agtctattat aggtgatgtt
    29101 aaatcttggt ttgatgaaat aaatgacagc aaattataaa aaattatgag aattttattg
    29161 tataaaagta aaaaattaaa aaatatataa aaagtgatta tttgttgtga aaatagtctt
    29221 gtttttaact aaattgataa aataaactaa aaaagatttg tacttaaaaa aaaaagaaaa
    29281 tacaagaaat atgcaatatc aacatttgga aaaaaaagta aaaatgtata caaaatacat
    29341 ataaaaaaac tattgcaaat tttatagaat gtttatatac ttaagtcaac agagatagaa
    29401 aaaagctatt tatagaaaac aaaggataaa ttgcttaatt ttaaattttc tgaaaaaaat
    29461 aataatccgg atgaagagta tgggagactg ctttataaaa agcgaccgaa gggacaagtt
    29521 taatcttact tacccaaaag attagatgaa attctcaggt aaaggtgact gtacttggac
    29581 ggggctctga aaatgatttt aacagggaag acaaattggg agatttgttt ttcctgtttt
    29641 ttatttatca agtaataaat aaaagtttga aagagagtaa aaaaatgata ataattacga
    29701 ataatccaaa agtaaaagaa gaagttcaag gcagagaagt tttgtttaaa gacacaactt
    29761 atattggaat cttagaagca agtagagatt tgatacatga aggttatgaa cttttatctc
    29821 atccactcta tgggagtgta aagccaaatg aaacacctta caggactgtt atactaaaga
    29881 aaggaaatcg tttggatata aactctttaa ccttaattga agaagcaatc atcactgcaa
    29941 gtaaatttca gaataataaa aagactccaa aatggacaga aagtgttcaa gatgatttta
    30001 gagtaattga ttatgatata ttttataata caatacaaag aatgcagtat gaataaaaaa
    30061 attgttaaaa agttattaaa acagcctaaa tattaaatct aatttaatat taaataaaat
    30121 gagaaattta attggggagg ataagtcaaa atggaaaatg tgtatgactt agtaataatt
    30181 ggttcaggac cagcagggct tgcagcaggt ctttatggag caagagcaaa gttaaaaact
    30241 ctaatcctag aaaaagacaa gactggtgga caaattgtaa taacacatga aattgcaaat
    30301 tatccagggt cagttccaaa tgcaacagga ccaagtttaa tagctagaat ggttgaacag
    30361 tgtaaagagt ttggagcaga aatgctaaga gacaacattg ttgatactga gctagatgga
    30421 gatataaaag ttttaaaagg ggaaaaggca gaatatagag caaaagcagt aataatagga
    30481 acaggagcaa caccaagaaa gattggttgt cctggagaaa aagaacttac tggtaaaggg
    30541 gtttcatatt gtgcaacttg tgatgcagat ttctttgaag attttgaagt atttgttgta
    30601 ggtggaggag acagtgcatt agaagaagca atgtatttaa ctaaatttgc tagaaaagta
    30661 acaattgtac atagaagaca aggatttaga tgtgctaaga gtgttgaaga aaaagcaaaa
    30721 gctaatccaa agatagagtt tttattagat acagtaattg aagaaatcaa aggtgatgga
    30781 atattagaat cagttgtatt taaaaataaa gttactggag aaactcatga atactttgca
    30841 gatgaagaag atggaacaat gggagtattt gtgttcgttg gattagatgc tcaaactgat
    30901 ttattcaaag gtaaagttga tatggatgaa aaaggttaca taataactga tgaagatatg
    30961 agaactaaca taccaggcgt atttgcagca ggagattgta gatctaagac tctaagacag
    31021 gttgtaactg caactaatga tggagcaatt gcatctatag tagctgagaa atacattgat
    31081 gaaaaatttg ggaactagaa tctgtaaatt aagaaatagt gtctgtaaat ttaatgaata
    31141 atatttaaaa ttaaaattaa tatttaaaat aaaaataaaa atttcaaggg ggaataagaa
    31201 aatgttagat ttagataagg ctacatttga agaagaagtt ttaaatgcag aaggttttgt
    31261 atttgttgat ttttggagtg aaggttgtga gccttgtaaa gctttaatgc cagatgttca
    31321 taaattagct gagacttatg gggataaaat aaaattctgt aagatggata caacaaaagc
    31381 tagaagatta gctataaaac aaaaagtatt aggtcttcca actatggcta tatacaaaga
    31441 tggagaaaaa gtagacgaag ttactaagga tgatgctaca gttcctaata tagaaaacat
    31501 gattaaaaaa tatctttaat atgttgataa attattaaca ttaaaagaaa aaatctattt
//
LOCUS       DAYATN010000033        31371 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.033, whole genome shotgun sequence.
ACCESSION   DAYATN010000033 DAYATN010000000
VERSION     DAYATN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.033"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(105..1319)
                     /locus_tag="ACGI8A_001479"
     CDS             complement(105..1319)
                     /locus_tag="ACGI8A_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0179481.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(1784..2719)
                     /locus_tag="ACGI8A_001480"
     CDS             complement(1784..2719)
                     /locus_tag="ACGI8A_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HFH0179482.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            3007..3573
                     /locus_tag="ACGI8A_001481"
     CDS             3007..3573
                     /locus_tag="ACGI8A_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0179483.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            complement(3764..5800)
                     /locus_tag="ACGI8A_001482"
     CDS             complement(3764..5800)
                     /locus_tag="ACGI8A_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HFH0179484.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPAGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(5969..6646)
                     /locus_tag="ACGI8A_001483"
     CDS             complement(5969..6646)
                     /locus_tag="ACGI8A_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFH0179485.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            6989..7117
                     /locus_tag="ACGI8A_001484"
     CDS             6989..7117
                     /locus_tag="ACGI8A_001484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179486.1"
                     /translation="MSRIERRKKDRESKLAKVKTALSILPILINLIDKLIKLLKDL"
     gene            7363..7539
                     /locus_tag="ACGI8A_001485"
     CDS             7363..7539
                     /locus_tag="ACGI8A_001485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179487.1"
                     /translation="MEQKESKIVYSKGGSGTYSAKISLPLSQLEKMGFTREKRKAVVI
                     FKEDEIIIKKSDEE"
     gene            7700..7828
                     /locus_tag="ACGI8A_001486"
     CDS             7700..7828
                     /locus_tag="ACGI8A_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179488.1"
                     /translation="MGRLERRKNKRENKFNKIKNAFSFTLVLINLVLAILRLIKEL"
     gene            8057..8242
                     /locus_tag="ACGI8A_001487"
     CDS             8057..8242
                     /locus_tag="ACGI8A_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFH0179489.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(8284..10632)
                     /locus_tag="ACGI8A_001488"
     CDS             complement(8284..10632)
                     /locus_tag="ACGI8A_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFH0179490.1"
                     /translation="MKNLSKKAVILSLSLVVVSPLVHKINAQDIEKNREVRYETRKAN
                     NLENMTMEEAFPDENFRKVICDKLSILDDGNPIGISQKAIIESTKGLTLGSKSIKNLS
                     GINYFIGLENFSCRGNNLTSLDLSSNINLKELYCNENQLTSLDLSNNRELTTLHCGEN
                     NLTDLVIENSKLDELNCRKNNLKSLDITKATNLTTLLCFENELTSLNLSKNQKLKILK
                     CDYNNLNDLDISTNLELQDLNCSFNQLENINLDNNKELVKLICHNNKLQNLNLKNHEK
                     LVKLYCNQEQLSTLNVENCINLEDLTCSRSNLKNLDISSNKNLKYLECVANKLTNLNI
                     ENNIELLELYCYDNEIKALDISKNIDLENLTCYKNKLDSLNTSKNKNLKYLYCSQNEL
                     TSLDVTKNTELVRLYCGENRLTSLDVSKNTKLKELDWSNQKKSTSTGGSSGSGGGSSS
                     TNEESSEPTSTPSKEKLTGVDRNETSVKISQKGWNKADNIVLINDSSISDALSATPFA
                     KSKDAPILLTKNNNLNKLTEKEINRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRI
                     SGNDRYETSIKLAKELDKNSNLSKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQND
                     DMKDIKDLIANKNISKSYVIGGESLFNNKEVNNTLPSVTKIAGSDNNKEVNNTLPSVT
                     KIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLVDALSVGVLAGKTESPVV
                     LVGNGLDNSQKELIKNKKFKNITQIGGNGNEKAFNEVENLVK"
     gene            complement(10789..11601)
                     /locus_tag="ACGI8A_001489"
     CDS             complement(10789..11601)
                     /locus_tag="ACGI8A_001489"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0179491.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            complement(11680..12543)
                     /locus_tag="ACGI8A_001490"
     CDS             complement(11680..12543)
                     /locus_tag="ACGI8A_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFH0179492.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            complement(12804..13061)
                     /locus_tag="ACGI8A_001491"
     CDS             complement(12804..13061)
                     /locus_tag="ACGI8A_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFH0179493.1"
                     /translation="MDNLISFIPEQLLILVAALYVLGVGFKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            complement(13065..13355)
                     /locus_tag="ACGI8A_001492"
     CDS             complement(13065..13355)
                     /locus_tag="ACGI8A_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179494.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            complement(13386..13952)
                     /locus_tag="ACGI8A_001493"
     CDS             complement(13386..13952)
                     /locus_tag="ACGI8A_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179495.1"
                     /translation="MTFKELVNKVRNLVLEAKKVTIEDTENKFTSENVEEALKECINR
                     ADEAFQEADSGKELIATAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITIDS
                     NKTGFSVHSYDTSYEPYPFTGYYIACFA"
     gene            complement(13986..14168)
                     /locus_tag="ACGI8A_001494"
     CDS             complement(13986..14168)
                     /locus_tag="ACGI8A_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179496.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAIED
                     YIIKNSGVVEGAEPTI"
     gene            complement(14168..14473)
                     /locus_tag="ACGI8A_001495"
     CDS             complement(14168..14473)
                     /locus_tag="ACGI8A_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179497.1"
                     /translation="MTEYVEVGRRIFFDEEGKIIFYEGQSKGNVPDRKNIKKIEYIDL
                     EYDYVDYDKYKIIGIDIRTKQPILEEIPVYMSEEGKRIQELENQILIAENEKSGGLL"
     gene            complement(14497..15102)
                     /locus_tag="ACGI8A_001496"
     CDS             complement(14497..15102)
                     /locus_tag="ACGI8A_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179498.1"
                     /translation="MIEKLNENASLSDLITTFENVKDELQIGKNNIASTLGNPFVGTD
                     KLSVTKTKIQTLKNTLAISLSMKNISAQSTETLHSLIGKVAGIANIFIAQGECTQVGE
                     KTFQLYQVDNNTPKTCSANAISFNLAFTPKFIIVYRNAYNIGLCSFVFYSSFRNIITA
                     VPQPSNFGEMYMPNTFDCVVGHTCILPVKDYKIPHFFYAIA"
     gene            complement(15313..15447)
                     /locus_tag="ACGI8A_001497"
     CDS             complement(15313..15447)
                     /locus_tag="ACGI8A_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFH0179499.1"
                     /translation="MWYKIIKKYYEDELWTKEQVKTAVEKNKITTIEYKNIVGEDYIV
                     "
     gene            complement(15448..15813)
                     /locus_tag="ACGI8A_001498"
     CDS             complement(15448..15813)
                     /locus_tag="ACGI8A_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179500.1"
                     /translation="MIKIWDKKEKINGVSAEEILKGNYDFQTSEVFLILDDYNRVTNI
                     ESVNTIKSIYKLDKNLTALETAEKYLEMHKKQEEDIKAHEELEKNSKNATIATHEEVK
                     ALREETATLTFAMMEKELI"
     gene            complement(15831..16964)
                     /locus_tag="ACGI8A_001499"
     CDS             complement(15831..16964)
                     /locus_tag="ACGI8A_001499"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179501.1"
                     /translation="MTIKLEEDSSIKEVMDSFENIKNDLQNAKDNLASTLGSPFVSTD
                     SFSTTKTKIQTFKSTLATNLTKKGVSTSSNETVQNMVSKVDDIELGNTDIPNWYKPKN
                     IIFDGVTSALQQYPPCVAVENYIYFLCNNSLGLSLLRYDTLTNVNSVLAKPSVCDDAL
                     MVYYDGYIYRIGGYSNSSANSTCQKYNIKTNTWSYISNMITPRGGTCGNTYSNEIHVL
                     YGYTDTNSNSTASAISEYYKISTNTWSTKTSFPPSRDRRLFVCAKTGTYYFCITGATD
                     PNNIWETYINRYDAGNGALTKIEGWYKSFATSINDMLYTVYYGDTYLYVKGYNQNGRV
                     FSKEYVKKNSTFPYNPLLKAVSVKNKYIYYYENNMYKCFIPEL"
     gene            complement(17157..18302)
                     /locus_tag="ACGI8A_001500"
     CDS             complement(17157..18302)
                     /locus_tag="ACGI8A_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HFH0179502.1"
                     /translation="MATKLEENAKLREVIDTLETTKDDLQTVKNNIADVLGLPFTGND
                     KLETTKNTLSSIRSTFVSNLSKKGVSTVSNAPFKTLAENVGKIEQGNMIVPPWYVPKN
                     IFIKALSTAVKDKTPCVSAEKKIYFINGYTGTSGLNNLCYDTVLNTTTTKNIPSSFVE
                     SALFFYDGYIYKIGGFSGYSAPTAICQKYNILTSSWTEIANMPTARGSACCEMYSNQI
                     HVLYGGEDSSSSQAVRVVEYYNTTLNTWSTKSTIIDSKQSHYFVCAKVSTYKIVVFGL
                     KSSISSHYFSTINYDPVSGTYSYAGEGFDSNTFAVQLGSTLYVSDYGYKAGSGKCGVV
                     IFSSSGIKKLTFDKGAVPNYSYFGASVDGRQIYFILNSEITCFIPEL"
     gene            complement(18319..19395)
                     /locus_tag="ACGI8A_001501"
     CDS             complement(18319..19395)
                     /locus_tag="ACGI8A_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFH0179503.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETAKTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAESG
                     FKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEIRSEARSELNVYVINGNA
                     ETHFINATVDDNRVSEMTTYSSKKIEDSISSIQLIDTSISITDANDRFTSDKLDGVLE
                     EIMVEISGQRTKGITIVNNLIDMI"
     gene            complement(19407..20024)
                     /locus_tag="ACGI8A_001502"
     CDS             complement(19407..20024)
                     /locus_tag="ACGI8A_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFH0179504.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHEKLNDLSS
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNY
                     MNFGITTLIGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            complement(20017..21066)
                     /locus_tag="ACGI8A_001503"
     CDS             complement(20017..21066)
                     /locus_tag="ACGI8A_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFH0179505.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGQLIPIEYVDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TIDSNSIVVRGEVVG"
     gene            complement(21067..21486)
                     /locus_tag="ACGI8A_001504"
     CDS             complement(21067..21486)
                     /locus_tag="ACGI8A_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFH0179506.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            complement(21491..21751)
                     /locus_tag="ACGI8A_001505"
     CDS             complement(21491..21751)
                     /locus_tag="ACGI8A_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HFH0179507.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESINPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKVVLIRMQGGQQYLVLDRM"
     gene            complement(21765..23657)
                     /locus_tag="ACGI8A_001506"
     CDS             complement(21765..23657)
                     /locus_tag="ACGI8A_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFH0179508.1"
                     /translation="MADDLVLANDRDVRLIIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKLSRVEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            complement(23650..24351)
                     /locus_tag="ACGI8A_001507"
     CDS             complement(23650..24351)
                     /locus_tag="ACGI8A_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFH0179509.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYNIVEDAENGSDTKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWLICKKQLGNGSLYKKVYELNKSMMDKANKGKN
                     LSKYTIYKGQVLKLG"
     gene            complement(24367..26760)
                     /locus_tag="ACGI8A_001508"
     CDS             complement(24367..26760)
                     /locus_tag="ACGI8A_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFH0179510.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIESITKAADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QIAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPITFSQALTMMKNDAYMIFSETLSKISGALQSVRFSEIVVSMRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVQGITTGTVLIIQGLGWITNAALNVANVFAQNWSIIAPAVYG
                     VVAAIAIYKGILLASAIVTGVSSFVNALYALGAYKACAALAAQELALFGKISAQTIEA
                     MVTAQATAAQYGLNAALYACPLVWIVLGFIAVIAVIFMVVAAINKFAGTSLTVLGVIV
                     GAVFAAVATIQNIMIWLFNRCVDVNEGIANGWNQCVYLMKQAIAKGVIFIIEKMASLN
                     DSVNNAGNALGKAFVDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYI
                     KQQIDSLNRWVGDAPEKVKLERMGYKDIGAEFQKGNALGTKWQNAISNKLKDTFDINK
                     MLEDAKDKLGLKDLWDKDNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKY
                     LRDIAEQETINRFTGVNIKIDMNNTNNINSEADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            complement(26825..27340)
                     /locus_tag="ACGI8A_001509"
     CDS             complement(26825..27340)
                     /locus_tag="ACGI8A_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFH0179511.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREEE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     repeat_region   27471..28292
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=27536..27564
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            complement(28368..28517)
                     /locus_tag="ACGI8A_001510"
     CDS             complement(28368..28517)
                     /locus_tag="ACGI8A_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179512.1"
                     /translation="MHENLLDIDRIELIKELGNIFEKMKNENPDEFYRFVSLVKEECR
                     KKREK"
     gene            complement(28569..29384)
                     /locus_tag="ACGI8A_001511"
     CDS             complement(28569..29384)
                     /locus_tag="ACGI8A_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFH0179513.1"
                     /translation="MMNELMNFEGKDIEVFEFEGQILFNPKHVAECLDISDVNSSIRK
                     FNDNQVVKLTNSKMHNMQFRKLHNTGENFLTESGVYKLIFKSKKEEAERFQDWISDEV
                     LPAIRQTGAYITNNADPEKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSKLLTA
                     KTLYKKAGIDLPIEINEEEHYFDTKQIASKLKLYSKSNKPAQMAVCEIIKKIDLEDSE
                     VKGVWETNGSWTGTVNKYTESVIDKIRNWIEENNRPTKIAGEKKNYHVVYKIE"
     gene            complement(29502..29684)
                     /locus_tag="ACGI8A_001512"
     CDS             complement(29502..29684)
                     /locus_tag="ACGI8A_001512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179514.1"
                     /translation="MSNKLGRPPKENSKKLRFEVRLNQEQADILNECAENLKISKTDV
                     VIRGIELVKEKIDKNK"
     gene            complement(29871..30395)
                     /locus_tag="ACGI8A_001513"
     CDS             complement(29871..30395)
                     /locus_tag="ACGI8A_001513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179515.1"
                     /translation="MWGKFKKLSLLKKILVIFLIYFVVFTVSMMIHQAIRDSKNRDEV
                     NEGNITKENIISEREKEDIYKQEMQAKVDSMIPEDLKDKTTYYVNILNPTKGEGYIVS
                     IQVENSRFNDENECRNFTKEFVNNIKDMNDIHSVRISFIVDVTLTYNVFLDDWNNIKN
                     NVNLIDDLDFSSGN"
BASE COUNT         9897 a         5481 c         3438 g        12555 t
ORIGIN      
        1 tctaaataac tgtttaagac cgacaaaaaa ggtgattcaa atttttattt tgaaacactt
       61 tcttttaatt tgattaatct ttaagtccta tattaaattt ttacttattc tttatttttc
      121 aacatttttt ccatatctaa aatcttttca attacatcct taaaaattgg ttcttctcta
      181 agctcattat aactgtcatc attaagtaca attaacaagt ttgtatatat attaaaagta
      241 tataaatttt tagactctac cttatcaaaa aatctcattt gacttacatc caaactttca
      301 tgtataggat atttttctaa tatttctttt agactatata aagctttttt tgcttcttct
      361 ttcatgttaa aaaataaaaa aattagagat aattccatat aatccatata catactaaaa
      421 aatgcacctt tatcttcact gagcattttt tttatttcta aagaaagatt tgcatacccc
      481 agcaacttat tttttatata ctccatttct ctctcaattg catttttatt aaaatttcta
      541 ttctccaatt ttttctttaa ttccttgtat tgatttttgt atacattaat tattccctta
      601 caatccatgc ttatattaaa tacattacta aacaatttat tttgaagcaa tatttctgct
      661 tcttcaagtc tattttgctt taaatatatc atagctaaat taacacttgt atcccctatt
      721 gacttatgta tcatatttaa atacaactct gccttttcat aatcctccaa catacaatat
      781 gcgtttgata atataataag agctgtttct tttatttgga tatcatctgt atttctcact
      841 atatcttcaa gaatattcgt tgtttctttt actctgtcat taagttcact cttaccaaat
      901 ttatatgaat acatctgata cagcgaacat atccttagct tcaataaata gcttttagga
      961 tacttctcta tatacatatt ggaaagcttt tttacagatt ctaaatcttg tctaaattct
     1021 tcttttggtt cttctgttga taaatcaatt tcaattctag caaagcctga ttctaattct
     1081 ttataaattt tcattacttc ttcttcactt aaatcaatct tgtaatttaa aagcttatca
     1141 atagacacat taaaaaagtc tgctaataaa ggcaataatt ctatatctgg gtaagaattt
     1201 ccactctccc acttagatac tgctggtgtt gacacaccta taaactttgc taattgttct
     1261 tgagttaaat ttcgttcttt tctaagacac tgtatgactt tacctatttt aatttgcata
     1321 tatacctccc tagtacatct gttgtaattt aagtatataa aaaaaatatt tactagtaaa
     1381 tcgagggaaa gttaacaaaa tgtctgtaat ttaaccattg gttatatttt aatctaaaac
     1441 cctatctcat tgtcttgaaa tataaaatct attaattaag tttaagtata acctaattaa
     1501 tagattcaca ttaaatagca tttaaatatt tatattttct ctttactaca acttactata
     1561 tttaaacttt caaggaattt taaataaaat aaattccata tacaatgata ataatactga
     1621 gtgttaataa tttatttata ataacaccca cctattcaat aaatggcatt atagcaaaaa
     1681 atcacttctg ggctctctct tttctacttt gcatatacat aatccacttt tcaaatatta
     1741 tatataaata aatttaaagt aaatatatca ttatattttt ttattacaat atttctattt
     1801 tattatttaa ccaacaaatt atatcctcaa aaacttcttc tctatttatt tcattcaaca
     1861 attcatgcct tccatctttg taaagtttgc aagttacatc ttttactcct aattttatat
     1921 atctatccct tagtcttaag actcctttac cattttttcc tatggggtct ttatctccag
     1981 atattatgta aataggtata tcaagtggca ctttttttaa gttttcttta tcctcaattt
     2041 cttttaaccc ttgaactaaa tcataaaaaa agccacaact acacactact ccacaaaatg
     2101 ggtcatttat atatttttca acttgttctt tatctctact taaccagtca aacttagtct
     2161 tttcatttct tcttattatt tcgccgccaa atatcaaatt atttatttta ttacttttag
     2221 aacgtcttcc atacttttta atctcacgat tgattatcag atgagcaaga tttagaatga
     2281 ttccttgctt tccatttgaa ccacatagta taagaccaga taaattatta ctgtaatcca
     2341 ttatatatct ttgacttgca aatgaaccca tgctgtgtcc aaatagataa attggcagat
     2401 cttcattttc tttctttata atatttgtta aagtatacat gtcctctaca agacatctaa
     2461 atccttcttt ttcagctaaa tgtccaacat tttctattat ctttgcagtt ttcccatgac
     2521 ctctatggtc attgatatat actatataac catttttagt aagtaccttg gcaaaagtct
     2581 catacctttg tgcagtttca gccattccat gtgcaatttg gataactgcc tttggttttt
     2641 ttatattttc atcttcccat ttatatgtat atatatctaa accttcctct cctttgaaag
     2701 taaagtttgt acacttcata attatccctc cagattgatt ttattcagta tattaattta
     2761 tatattatat tacaagtatt ttcaagtcaa aatctttcta aaaaatttaa gaattattaa
     2821 ttcctcatag aagttatcat tggtacatag aagtttttac ataaaattat acaataattt
     2881 aattaattaa tagttacata aaaaagtaat actttaatat attttgacac catattgaca
     2941 ttagaattaa ttcatgatat tctgttttcg acaaaacaac atctaaaaat aaaggagata
     3001 tatttcatgg attattcaaa tgaactaaaa gaactctttt taatgaatca aacatatgca
     3061 actcttttta cccttacaaa caaaatacaa atagaaggag ataaatattt tggtatactt
     3121 acttcaagac agtatatgac tattttatca attcttcatt taccagagga agaaacaaca
     3181 ttaaataata ttgcaagaaa aatgggtaca agtaaacaaa atataaatag actagttgct
     3241 aatcttgaaa aaaatggata tgttgatgta attccaagtc ctcatgataa gagagcaatt
     3301 aatgttaaag ttacagactt aggaaaaaag gttatggtaa catgtagtag gactggaata
     3361 aactttatgg cagatgtatt tcacgaattt accaaagatg aattagagac cttatggagt
     3421 ttactaaaaa aaatgtatcg ttttaatggt gaagaacaag atggctttga agaagatgct
     3481 aattttatgg aatatgaaga aatagataaa attaaatctg aagcacttga agaatttgca
     3541 aaaagaagaa agagagtaaa taaaaacgat taatactatc gcatctataa tactactttt
     3601 ttatagtaca atattttata taactataat gtcattttta ttttttaaca aaacaatcat
     3661 tacatagatt aggtgttctc aggaactctt tttctgtttt ttgtacaaaa aaaggagatg
     3721 attagacttt tattttaaag catctccttt ttcactatta tgattattct actcctaaaa
     3781 tcatatcttt taatttttgt aaatcattaa ttggttgact acaactatta ctttgacaca
     3841 cgtaataact aatcttatca tccttcaatc tatagttatt taaaaaacct ataatagtgt
     3901 tttcttcatt gtatttcttt gctaaaaaag ttgcatttgg tataaaattt tcacttatca
     3961 attctttaaa ggcaattaaa tcagaatctt ccttaaaaat acatataatt tccttagtag
     4021 aataaagttc aaacatcaat gaaagcatat aaaagctgta gccagctgga gaactcttta
     4081 cattatccac atacagtttt aattgtttat atgacatttc ttcaagtcta ttatctccag
     4141 taattttcgc aagtcttata agattgtata attgaacaga atttcctgat ggaatagctc
     4201 catcatacaa atcttttggc cttgctatta aattttcact atctttacca tatatataaa
     4261 atccactttt ttcataatcc caaaaaaggt tgatacagct ttcattcaaa tttaaagcct
     4321 tttctaaata tttcatatta aatgttgact cataaagctc tatatacgcc cagattaaaa
     4381 aagcataatc atctaaatat gctaaatagt cggaacttcc atctctatat ctagctaata
     4441 atctacctga ctcatttaca agattattat ttataaagtt aagacactta tttgaatact
     4501 ctaaatatat gtcatttttt aaagtactat atgcctttgt taaagcaact atcatcaatg
     4561 cattccatga tgtaagtatc ttgtcatctt tatgcaaaga tgttctttct tttctatatt
     4621 caaatacctt tttactaagg tcagctatct tttcattatg tctctcatat tctttatttt
     4681 ttataagatt aggaatactt ttgccttcaa aatttccact actagttata tcaaaataat
     4741 tattaaagaa aattccatct tcttcaccca aaacttcaat tatctcaaga ggattaaatg
     4801 tataaaattt accttcttca ccttcactat cagcatcctg tgctgaataa aacccacctt
     4861 ctttatcctt catctctcta actacataat ctatagtttt tatagcaatt tctttataca
     4921 actctttctt tgttatttta tacgcatcta aaaaagctat agtaagcata gcattatcat
     4981 atagcatttt ttcaaaatga ggagccaacc actttttatc agtagagtat cttgaaaacc
     5041 caaatccaat atgatcaaac aagcctcctc tatacattcc atctaaagtt ttttccacca
     5101 tttttaacac atctttgtct ttttctatac tataatattt cattaaaaac ataagattct
     5161 gaggacttgg aaatttagga gcattaccaa aaccaccata cttttcatca taaatagcct
     5221 taaatactct aactgatgaa ctcaacatct cttttgataa atcaccctca gtatttttta
     5281 caccaaaatc atccttaaga gcttctatta tttcatctcc agattttatt aatatatctc
     5341 tgcttgtatt ccacttttca gatacattct ctaataaatc tataactcca ggtctattat
     5401 accttgaata ttttgggaaa tatgttcccg caaaaaaagg ttttttatct ggtgtcatta
     5461 ttatggtcat tggccatcca ccactacctg tcatagcttg gcaaactgtc atatacacac
     5521 tatctacatc aggtctttcc tctttatcta cttttattgc cacaaagttt ctattcatta
     5581 tttcagcaac ttcttcatct tcaaaagatt ctttttccat aacatggcac caatgacatg
     5641 ttgaatatcc tacacttaaa aatattggtt tatcctcttc ttttgctttt ttaaaagcct
     5701 catcattcca actataccag tttataggat tataagcatg ctgtaaaaga tatggagatt
     5761 tttcatttat tagattatta ggttttctat tactgtccaa aataccacct catttgttat
     5821 tttattatat gttttgcaat aaacaaatag atatacactt ataataagtg tatacccatt
     5881 tttaactata catatttaat ttttcatata attaattctt ctagtaaata atttttataa
     5941 ctactaaaac aaaataaaaa atatcaaatt atttttttaa aggtttttca aaagaaggag
     6001 gattaatatc tatcttagta tcgtgatttg gccctggagt agacaaatcg aaatataatt
     6061 tagaatcttc tgtaactcca aaatctctat ttgaacctgt atctttaact ttatttaaag
     6121 catctctaaa tagagcatta tgtgcttctt ctctattaag taagaaatct attgtttctt
     6181 ttacatattt atcatctatt tgtctataca agtactcata aactacctta gctctttgtt
     6241 cagaagctat atttgaaagt aagtcagcta ctaaatcccc tgtaactgta acgtaattag
     6301 cagtccaagg agctcctgat gagtttatta agactggtga tagaccagtt aatacatgtg
     6361 tttctatttc gcctgtatta actgcattat aatcaacatc atgtccattt aataaattta
     6421 tagtttgtgc taccatttcc atatggctaa gctcttctgc tgctatatca agaaataaat
     6481 cttttatttg agggtctttt attctaaaac tttgggacaa gtattgcata gctgctttta
     6541 actctccatt tgcaccaccc aactgttctt gcattaaaac agcatattga ggatttggtc
     6601 tttctacttt tacttctttt aataatgctt tatcgtgttt aaacatatga atacctccgt
     6661 aaattattat tcaaaattat tatttactta agttgtaata ttattctagg ttatttgttt
     6721 attgttgtta ttttttacaa gtgttggtat ttcaatgttt tattaaggtc agttttgttg
     6781 tttatgtttt attatgttta atttaaattt attctattat ttattttcgg tggcaaaagg
     6841 gtggcaagtt tggttttgtc accatttatt tttatttgta aaaaatatag tttatcaata
     6901 taactttatt gaaaaatatg gaaataaata ttgttttcgc cggttcggca tgttataata
     6961 taaatataga aagggggtga aaaggaaaat gagtagaata gaaagaagaa aaaaagatag
     7021 agaaagcaaa ctcgccaaag ttaaaactgc tctctctatc ttacccattt taataaattt
     7081 aattgataaa ctaattaaat tattaaaaga tttatagttt tgtaagctag gaagaattta
     7141 atcttcttcc tagtgacttc tttctaaata ttataacaca ttttctataa aatatgagtg
     7201 ataaagttta taaattctgt cttattatag ttgctataac tatagtaata gacataatac
     7261 ttttattttt acattttaat atcaacaata taataggatt aatagtaagc attttattgt
     7321 taatatttgt tacattacaa tataaaaaag gaggaaaata gtttggagca aaaagaatct
     7381 aaaatagttt acagtaaagg tggttcaggt acttattcgg ctaaaatttc tttaccttta
     7441 agccaacttg aaaaaatggg atttacaagg gaaaaacgta aggctgtagt tatttttaag
     7501 gaagatgaga ttataataaa aaaatctgat gaggaataac tctaaaataa gtgggtgtat
     7561 aatgtaaaca tccacttatt tttattatat tttgtatttt atattaatat attttataac
     7621 tttattgaaa aatatggaat atttattggc tttcacgtca cgtgatgata taatataaat
     7681 atagaaagga ggtaagaaag tggggagatt agaaagaaga aaaaataaaa gagaaaacaa
     7741 atttaataaa attaaaaatg ctttctcttt cacattagtt ttaattaatc tagtactcgc
     7801 aatactaaga ttgattaaag aattataagt ttcaatccta ggaggaaaat accttccttc
     7861 taggtagctt ctttctaata ttataacaca ttttctaatt taatatgaat aataaatttt
     7921 atagaatttg tatgattatt cttggtatca ctatagtttt aaatattatt aatataataa
     7981 taagttttac ttttaaagct cttattgctt tggtatttac tttattattg ctggcattta
     8041 ttaatagtag aaaggaatga tattattgga gcaaagaaaa cttagaattg gatttaataa
     8101 aagtggcaaa ggctctttta cacccagatt gatacttcca atgtcatgga ttaaagagat
     8161 gaatatctct cctgatgaaa gggatgttct tgttactttt gaggatggaa aaataattat
     8221 tgaaaaaact gaaaatgaat aaaaaaagga ggtagtaact atttccagtt actacctttc
     8281 aatctattta actaagtttt ctacttcatt aaatgctttt tcatttccat ttccaccaat
     8341 ttgagttata tttttaaact ttttattctt gattaactct ttttggctat tatccaatcc
     8401 atttccaact aatactactg gagattcagt tttacctgca agcactccta ctgataaggc
     8461 atctactaaa tcgtcttgtt tattcattcc attcttagct acatataagt cattaagtgt
     8521 atctttgcta tagaaatgac ttattacttt tgagttagtt tctgttctat cagaacctgc
     8581 tattttagtt acagatggta atgtattatt cacttcttta ttattatcag aacctgctat
     8641 tttagttaca gatggtaatg tattattcac ttctttatta ttaaataaag attctccacc
     8701 tattacatat gattttgata tgtttttatt agctattaag tcttttatat ctttcatatc
     8761 atcattttga ttagtaagaa gaattggcat ttcctctttt gctgatatag ctcccatact
     8821 tactgcatca gctagtccct tttctccatt taccactact acttttgaca ggttagaatt
     8881 tttgtctaat tcttttgcaa gttttattga tgtttcatat ctatcatttc cagatattct
     8941 aattacattt aatccttttt tctttaaatc agacactact ttttcatcaa cagactttaa
     9001 tccgcccact atgtacacat tttttgcttc taatctattt atttcttttt ctgttaattt
     9061 atttaagtta ttatttttag taagtaatat tggtgcatct ttagacttag caaatggtgt
     9121 tgctgataaa gcatccgata tgctagagtc atttattaac actatattat cagctttatt
     9181 ccatcctttt tgacttattt tcactgatgt ttcatttctg tctacacctg ttaatttttc
     9241 cttagatggt gttgaagtag gttcagagga ctcttcattt gtagatgatg aacctcctcc
     9301 actccctgac gaacctcctg ttgatgttga ttttttttga tttgaccaat ctaattcttt
     9361 taattttgtg tttttgctta catctaagct ggttaatcta ttttccccac aatatagcct
     9421 tacaagttct gtattttttg ttacatccaa gctagttaat tcattttgtg aacaatataa
     9481 gtatttaaga tttttatttt tacttgtatt taaactatct aatttatttt tataacaagt
     9541 taaattttct aagtcaatat ttttacttat atctaaagct tttatttcat tgtcataaca
     9601 gtataattct aaaagttcta tattattctc tatattcaaa tttgttaact tgtttgctac
     9661 acactctaga tattttaaat tcttattgct acttatatcc aaattcttca gattacttct
     9721 actacaagtt aaatcttcca aattaataca attttccaca tttaaagtac ttaattgttc
     9781 ttgattacaa tatagtttta caagcttttc atgatttttt aaatttaaat tttgcaattt
     9841 attgttgtga caaattaact taacaagttc tttattatta tctaaattta tattttctaa
     9901 ttgattaaaa gaacagttta aatcctgtaa ttctaaattt gtagatatat ctaaatcatt
     9961 taaattatta taatcacatt tcaaaatttt taatttctga ttcttagata agttcaaact
    10021 agtcaattca ttttcaaaac ataaaagagt tgtcaaatta gttgctttag ttatatctaa
    10081 actctttaaa ttattttttc tacagttcaa ttcgtctaat ttagaatttt ctataactaa
    10141 gtctgttaga ttattttctc cacaatgcaa cgttgttaac tctctattat tacttaaatc
    10201 tagacttgtt aattgatttt cgttacaata caattctttt aaatttatgt tgctacttaa
    10261 atctagactc gttaaattat ttcctctaca actaaaattt tctaatccaa taaaataatt
    10321 aattccagat aaatttttta ttgatttgct ccctaaggtt aaaccttttg tagattctat
    10381 aatcgctttt tgtgaaattc caattggatt tccatcatct aaaatactta gtttatcaca
    10441 aattactttt ctaaaatttt catctggaaa agcttcttcc attgtcatat tttctaagtt
    10501 attagctttc cttgtttcat atctaacttc cctatttttc tcaatatctt gtgcatttat
    10561 cttatgtacc aaaggtgata caactaccaa tgaaagtgat aaaataactg cttttttgct
    10621 taaatttttc atccccacac ctcattcaaa taatatttac ccacttgaat tgtatcacat
    10681 aatattaacc ttagaaacaa actttaatat tattatataa taaattattt ttttattatg
    10741 aatgaggcat tagaagataa caactacttt tagttgctac tttttaattt atctattaat
    10801 aaaatctaat gccttataaa gtgtatcaaa tctatcatta ccctttatca tagtaaattt
    10861 ttctttagtc atagaaccaa tcttctcaca tgctgcacca ccaatgacat aaagattttg
    10921 cgtctgacct ggcacgtaat cttttatatc acatatcagt attttcccat cattataacc
    10981 ccaaccaact acagttgcag agattttgtc aacttctcca tcataaacaa ttgtatgttt
    11041 gtacatctgt ttaactccct cattatttat atttttattt aatacacctt ctacaattaa
    11101 cttagcaata ccttcatgac ctagtttctt agctttctca taatcttctt tattatcaca
    11161 gaagaaactt tcaattaata ctgctgtagg atttgaacta tttaagatat ataatccttt
    11221 atctaattta gctcttctat ttctaaatac tgtacctagc ttattacata ttctagttgc
    11281 atattctaaa cctttattac tataatatag aacttctgaa ccttttcctt gaccatcact
    11341 tgcatttaga tgtagttcta taagtaaatc atatcctcca ctattaactc taggtatttt
    11401 ataagtcttt tcttctgctt tagttttaaa ctgcttttca gggcatatta ttacatctgc
    11461 cttatgaccc tcttttctaa atgtatctgc taatactggt gcaagagatt tattgtattg
    11521 gtattcgtta actactccat cagcagaagt gcatgcacca ctttttaaaa tactgtgtcc
    11581 tactgtaata catattttca ttatttattt tcctccttct tttcaataaa aaaacactta
    11641 catatagtaa gtgtttataa acttcttaaa gtaactattt tatttagcca atatctctat
    11701 ccaattatta ggaaaaccaa taagctttaa gtctatagaa ttattataat cattaattaa
    11761 cttttgaagt tttataaaaa aactattcca aattaattta tccatagtca aatgcttcat
    11821 agcaagtata taagaaaata tctttttatt atccaagtta tactttttat attcgttttt
    11881 tattgttata atacggaaat tattattata tattctacca taatgagcgc attgatttct
    11941 gacctgtgtt aatgattgta accaagattt aactaataaa gtattcactc tacacaaatt
    12001 attttttatg tatctagtat cttctggcaa catgtttgaa tataattttg acaacatacc
    12061 aaaagtcatt atttccgttg caacccaaat gggaagtttt ccttcatatt tttctttatg
    12121 gtgtataata aaaagcttat ctgaattatt acttttttct ctctctaatg ctgtcaaaaa
    12181 attaatatag aatttttcat ctttgaaact ctccctttct agatatccac aagcaccatg
    12241 tttaattgct aatgtatatg caatgtatgt cctaaatgct atttctatgc tccctaacaa
    12301 atctgttaat aatatcctaa attccttatt aaacctatat atatcataaa cttcttcaaa
    12361 cgtagttcct tctttatatg agccatcatc attcttaaaa cttagaagat atgctgtaaa
    12421 cctataataa ttgacattac ttaatacaaa tttagcatct tctttatcat ttattattaa
    12481 tcctctactt tttaaaatat ctatttgttc atcaaatgtt ttttcttctt ttacttcaac
    12541 cattgttttc accttttcat agatatactt ttatataata aaacccacca attaatatta
    12601 gtgggtaaat ttatctgtcc cgcctatttg agctataaaa gaagcttggc gggttccttc
    12661 aaaaatcaat gtcccgtata tttgagcata taaatgcttt acgagttctg tactattatt
    12721 atatacttat tagctataaa agtatactct atttaatgaa attttttaaa tttttgtgag
    12781 taataatatt tttgttaatt tcattacttt tcaccatcct tcaactgttt gtaagtttga
    12841 tttataccta ttgcaactcc ccaacaaatt acaccttgta agactgcaac aggatttaat
    12901 cctagcatcc atattgagaa acctattcca agtatcagta acactactgg aatgtattta
    12961 ttatctaatt gtttatattt cttgaatcca acacctaaaa cataaagagc agctactaaa
    13021 attagcaact gctctggtat aaaacttatt aaattgtcca tcttttattt tcctccaatt
    13081 ctatttataa tttcatcaat tctaagatgt gcttgttttg tacttgcttc tacttttgca
    13141 actcgttcac tgatatctaa gaaacttgta ttaaactttg ctacatcatg ctttatatct
    13201 cttacattct cacacagaaa agttatttgt tgttctaatt ttgtagttgt ttctgtatca
    13261 tcttgtatct ttctgtttga gctatttcta aatgctaagt aggatattac aactcctgca
    13321 actgcacata atagatttat gctaatttct tccatacatc ctcctagttt taataatata
    13381 atttattatg cgaaacaagc aatataataa cctgtaaaag gatatggctc ataactagta
    13441 tcatatgaat gaactgaaaa accagtttta ttgctgtcta tagtaatcca tgcacgttca
    13501 gaacctcctg tattacttcc taaagaacta ccactgggat tataataata tacacttctt
    13561 ctaaagtaaa catagacaat aaggtaactt gcactcttgc caaaattagc agaaaaagga
    13621 ttaccatcat atccatcaat aggaccatat ctaatagaat atttgctttt caattgagtt
    13681 tctaaattgc ttatattgct cttaaatcct gtaatatagt tcgcataatc ttgaaatgtt
    13741 tgttctgatg tagcaggaga gccgatagca gttgcaatta actcttttcc actatcggct
    13801 tcttgaaaag cctcatctgc tctatttata cattctttca atgcttcttc tacattttca
    13861 cttgtaaact tattttctgt atcttctatg gttactttct ttgcttctaa tacaagattt
    13921 ctaactttat taactaactc tttaaaagtc atttagtcac cttctttcaa taaaaaaaga
    13981 accctctata tagttggttc tgctccttct actactccac tattttttat aatataatcc
    14041 tctatagcct ttctgtactc tgtgttagtc acgtcatcta actgaaattc tctatttttc
    14101 aatgggttta accctccgtt taatattctc tctgctaata ttcttactac aacattattt
    14161 atattcatta taatagtcct cctgattttt cattttctgc aatcaatatt tgattttcta
    14221 attcttgtat tctctttcct tcttcactca tatatactgg tatttcttct aagattggtt
    14281 gcttagttct tatatctatt cctattattt tgtacttatc ataatcaaca taatcgtatt
    14341 ccaaatctat atattctatt ttttttatat tttttctatc tggaacattt cctttcgatt
    14401 gtccttcata gaatattatt ttgccttctt catcaaaaaa tattcttctt cctacctcta
    14461 catattcagt catagtttcc tcctttaact tattttttaa gcaattgcat agaaaaaatg
    14521 tggtattttg taatctttca ctggcaaaat acaagtgtga cctactacac aatcgaatgt
    14581 gttaggcatg tacatttcac caaaattgct aggttgaggc actgctgtta taatattacg
    14641 aaaagatgag taaaaaacaa aactacacaa acctatattg taagcatttc tatagacaat
    14701 aataaatttt ggagtaaaag ctaagttaaa acttattgcg tttgctgaac aagtttttgg
    14761 cgtattatta tctacttgat agagttgaaa agttttttct ccaacttgag tacattctcc
    14821 ttgtgcaata aaaatatttg cgattccagc tactttacca attagtgaat gaagtgtttc
    14881 agttgattga gcagatatat ttttcataga caaactaatt gctagtgtat ttttcaaggt
    14941 ctgtattttg gttttagtta cacttagctt atcagttcca acaaaaggat ttcctaatgt
    15001 actagctata ttatttttac caatttgcaa ttcatctttc acgttctcaa aagttgttat
    15061 taagtcactt agactagcat tttcgtttaa tttttctatc ataaaaatat cacctttcta
    15121 tttttagtaa ctgtttgtca agaaatggtt actttttaaa atagaaaagt gattgatttt
    15181 aagtaatttt ttcaaagtat atatagtata aataatttta tttttaatag aaaaacaagc
    15241 aaaataaaca agtataaagc tttttagtaa ctctttcttg acaaatagtt actatataaa
    15301 attaaaaaag gactatacaa tgtaatcctc tccaacgata tttttatatt ctattgttgt
    15361 tatcttattc ttttctactg ctgttttaac ttgctcttta gtccataatt catcctcata
    15421 gtattttttt attattttat accacatcta tatcaactcc ttttccatca ttgcaaaagt
    15481 taatgttgct gtttcttctc ttaatgcttt tacttcttcg tgtgtagcta tagttgcatt
    15541 tttagaattt ttttctagtt cctcatgtgc ttttatatct tcttcttgtt tcttatgcat
    15601 ttctaaatat ttttctgctg tttctaaagc agtcaaattc ttatctagtt tatatataga
    15661 tttaattgta ttaactgact ctatatttgt aactctgtta taatcatcaa gtattaaaaa
    15721 tacttcactt gtttgaaaat cataattacc ttttaaaatt tcttctgctg aaactccatt
    15781 tattttttct tttttatccc atatctttat cattttttat tttctccttt ttataattca
    15841 ggtataaagc atttatacat attattctca tagtaataga tatatttatt cttcactgaa
    15901 actgctttaa gtagtggatt gtatggaaat gtagaatttt ttttaacata ttctttagaa
    15961 aatactcttc cattttgatt gtatcctttt acataaagat atgtatcgcc ataatacaca
    16021 gtatataaca tgtcattaat acttgttgca aaacttttat accaaccttc tatttttgta
    16081 agagctccat ttcctgcatc atatctattt atataagttt cccaaatatt atttgggtct
    16141 gttgctcctg ttatacaaaa ataataggtg cctgtttttg cacatacaaa taatcttcta
    16201 tctcgagatg gcggaaagct tgttttagtt gaccaggtat ttgtactaat tttataatat
    16261 tcactgatag cacttgctgt tgaattacta tttgtatctg tgtaaccata caaaacatgt
    16321 atttcattag aataagtatt cccgcatgtt ccacctctag gtgtaatcat attagaaata
    16381 tacgaccaag tatttgtttt tatattatat ttttggcaag ttgaatttgc cgaactattg
    16441 ctatatccac ctatcctgta aatataacca tcataataaa ccattaaagc gtcatcacaa
    16501 accgatggtt tcgctaagac actattaaca tttgtcaatg tatcatagcg taaaagcgat
    16561 aaacctaaac tgttattgca aagaaaatat atatagtttt ctactgctac acatggagga
    16621 tattgttgta atgccgaagt aactccatca aaaattatat ttttaggttt ataccagttt
    16681 ggtatgtctg tatttcctag ttcaatatca tcaactttac ttaccatatt ttggacggtt
    16741 tcatttgaag aagttgaaac acctttttta gttaaattag ttgctaatgt actcttgaaa
    16801 gtttgtattt ttgttttagt agtactaaat gaatcagttg aaacaaaagg acttcctaat
    16861 gtactagcta agttgtcttt agcattttga aggtcatttt ttatattctc aaaagagtcc
    16921 ataacttctt ttatacttga atcttcttct aattttatag tcattaaatt cctgcctcct
    16981 tttttaagta tcctttcgtt tagaaatggt tacttaatac ttttgtaatc tttgaattta
    17041 ctacattgta tattaaagta aattaattta aaatataaag ctgtttttag aatagtttaa
    17101 atttctgtcc cttttacttt tttaagtaac catttctaaa caaatagtta ctaattttat
    17161 aattctggta taaaacaagt aatctcacta tttaaaataa aatatatttg cctaccatca
    17221 actgaagctc caaaataact ataattagga acagctcctt tatcaaaagt taatttttta
    17281 attccactac tagaaaaaat aacaactcca catttgccac ttccagcttt ataaccataa
    17341 tctgatacat ataaagttga acccaattgt acagcaaaag tattactatc aaagccttct
    17401 cctgcataac tataagttcc acttacaggg tcgtaattaa ttgttgaaaa atagtgagaa
    17461 gaaatagaac tttttagccc aaacacaaca attttataag tgctgacttt agcgcaaaca
    17521 aaataatgcg attgttttga atctattata gttgattttg tagaccaagt gtttaatgtt
    17581 gtattataat attcaacaac tctgactgct tgagatgaag aagaatcttc tccgccatac
    17641 aaaacatgta tttgatttga atacatttca caacaagcac ttcctcttgc ggtaggcata
    17701 tttgctattt cagtccaaga actagttaaa atattatatt tttgacatat agctgtggga
    17761 gctgaatatc cactaaaacc acctatttta tagatatatc catcataaaa aaatagcgct
    17821 gattctacaa aggaagaagg aatattttta gttgttgttg tgtttaaaac agtatcatag
    17881 caaagattat ttaaacctga tgtacctgta taaccgttta taaagtaaat ctttttttct
    17941 gccgaaacac atggtgtttt atctttaacc gcagtagaca aagctttaat aaatatattt
    18001 ttaggaacat accaaggtgg aacaatcatg ttgccttgct ctattttacc aacattttca
    18061 gctaacgttt taaaaggtgc attactcaca gttgatacac cttttttact tagatttgaa
    18121 acaaaagtac ttcgtattga acttaatgta ttcttagttg tttctaattt gtcatttcct
    18181 gtgaaaggca aacctagaac atcagctata ttatttttga ctgtctgcaa atcatctttc
    18241 gtggtttcta atgtatctat cacttctctt agttttgcat tttcttctaa tttcgttgcc
    18301 atttttcact ctcctttatt aaatcatatc tattaaatta ttcactatag ttattccttt
    18361 agttctttga ccacttattt ctaccattat ttcctctaat actccatcta acttatcact
    18421 tgtaaatcta tcattagcat ctgtaatact tatactggta tctataagct gtatactgct
    18481 aatagaatcc tctattttct tagacgaata agtagtcatt tcagacactc tgttatcatc
    18541 cacagttgca ttaataaaat gagtttctgc atttccattt atcacataaa cgtttaattc
    18601 tgaccttgct tcacttctaa tttctataga attatcatct ataattttaa agtttgtaac
    18661 tacattttct tttgtagtag catctataat atttacaact attctctgtg ttaataaact
    18721 atgtgttaca gttgctttga atccactttc tgcatcctca acccaatcat caattgcgac
    18781 cctttgagtg tatgcaacat ttgagccacc tgcgattaat tggtcgattt tagtatttaa
    18841 ctctgtttta gcagtttcta tgttgcttgt taattctgtt ttagttgtat caattttagt
    18901 attaacagta cctattttag tttctaagtc ttgtatatct ttgagtgttg caaagattat
    18961 tgttgggtca attttaagtt ctatattatt tacattagat acaataagca cagttttaac
    19021 cttcatgtct accactgcac cttgttctat agaaggttta taacactctt tgtatttaga
    19081 aatggcaatt aaattatttt catcatctaa atatcctatt tctcttatca taaacccgcc
    19141 tacacttgat ggtattaaac tctctaatat tatacaattt ggtgcagttt catctgtagt
    19201 tgtatttcca atattgcctt cccataccac gtttttgaga gctgtctgac tctcagttgg
    19261 agtatattca ctccctcctc catcaccaag ttgaattttt acaaatccca ctttattacc
    19321 tgtgacactt gcatttgcta tctttgcttt ccctacatct gtaattatag tgtaataact
    19381 tttatctata gccaatatat cacctcctaa aatattgtta tctcttggta tccaactcca
    19441 ttgccagtta atacatcaat ttctccataa gtttctatat ctggtggact ccaagggtat
    19501 atagttattt cttgacctat tagggttgtt ataccaaaat tcatataatt gtctttactt
    19561 acaagcactc tagtgtaatc taaagtcatg ttgcaaggtt taatactact tacaaaagaa
    19621 tgaacctcct caaaccaatc ttgatttcta gcatcacttt caagatgtat attataagta
    19681 gcattattaa tagttaattc ataattgcct tctccaacta tactatctag ccagttcctt
    19741 aaaaatctct ctgagtaagg tagtttactt atatatttac taaaaatcct aaaccttcta
    19801 tcttctaaac tctcattact tttaggagtt atagacatta tcttttccca tctttttata
    19861 ccacttggag ttaaatcctc taaaaactgg tcacttgata ggtcatttaa tttttcatgt
    19921 agtgttttta tttctttgtt ttctacatta aatactttta tatattcttc tttatcttgt
    19981 agaatttgtg gtaagtaatt tattagatta atctctttat ccaactacct cacctctcac
    20041 tactatactg ttactatcta ttgttagatt agatttaacc tcatttatca ttgtatttgc
    20101 aatgtctaat actccatcaa tactaagtaa tctagtttca atttgagata tacggactat
    20161 taagttttct tcatcttccc aactcatgtt aagttcattt aaatagtcgt ctattgcttc
    20221 ttctgcaatt gattttatat tctcccaagt gtagccattt ttgtatgtta tctctgctga
    20281 tatatttata gttgtacttg taactccttc aactgtgact cggtgtccaa ttggtgctaa
    20341 tccaagacct tctccttggt tttgtagagg atcaatttct tcttgaacta aattaactaa
    20401 atcactagat ggaactttga agttagaatt aattattact aacttaacag tacctccacc
    20461 gtcccacaca ggataaacct taactcctcc aacatcttgt attttgttaa cttcatcttt
    20521 ataattttgc atatttccac caaaactctg tgagtttagg ctatcataat atctttgtct
    20581 taaactatct tcactttctt catcttctcc attaattagt atttcagtta actcagcagt
    20641 ttctaatttg tctacatatt caattggaat caactgacca agttcaaata caggtccagt
    20701 agtttcacat ttcattttat atgttttttc agatattctc tcaattgcca cataattgta
    20761 ttctcctaag ttgaatcttg aatcaatagg aatatctata ttaaaaactc cttttgcaat
    20821 tgtattggtt gcaggtaaag gtgtaatacc tctttcttta catctcttct ctaaatagta
    20881 ataactagca gtatctacaa aagtttggtc tagtaattca tccatggcaa tgtatgtttc
    20941 tgtaagttct atagcaacag gagcaagagc attatatatt atagaacctt cccttttatc
    21001 aaaagtatct ggtacactat ctaacattct tttaattata ttttcaaatg tcattaactc
    21061 aaacaattat acactcacca ccttctctgc ttttatattt ccatatttac tgtaaactgt
    21121 gaacttacaa tgtactttac cctttacatt ttgaaactca aaattatcta cattttcaac
    21181 cctatcatct tgaattagtg cttctttgat tcgtctttca agttcgggga ttacaaagga
    21241 tataggctct ccaataaggt cgttcaactc gactccataa ttccaactat atattagatg
    21301 ttggtatctc tctgtgttta aaattaaaaa gatggtttgt tttaatgctt caacatcatc
    21361 acaaatacca tctatcttag atttttctat atttaattta aaggtcttac ttggttcttg
    21421 ccttacatca aaattaatta ttgatacatc ttcaatgtca taatctaaat tatcgcttgg
    21481 taacacttca tcacatccta tccaatacta aatattgttg tcctccttgc atacgaatta
    21541 agactacttt atctcctatt tttttatctg tatatctttt aaatgtatct gtttgtatta
    21601 gaaaaatttc accaatagat agtttttgtt ctatcttaac tcttagagga ttaatacttt
    21661 ctattgttcc aaatacaacc ctcattgggt tgcttgtttc tactgcatcc attgcagctt
    21721 tttttattat ctgtaataaa tcttggctca tattgtcacc tcacttatat aaatcttctc
    21781 acatgtgtgt atgcttttcc ttttctataa gaattaacag actctatttt taccacatct
    21841 cctgtttgtg gtgaatgaat tatttgattg tttccaatat acattacaac atgattacta
    21901 cttcctccac caattctaca taataagtct cctgctttcc acttgcttct atcttttaaa
    21961 tctactgctt ttcctgcttt actttgtgca gaagcggtac gaggaatttt tatacctatt
    22021 tgtttataac accattgagt aaacccactg caatcaaaat tattaggacc ttctgctcca
    22081 tacacataat tacaacccag tttacttttt gctatactaa ttaatttatc ttctttagag
    22141 ttattatttg tactactttg gttattacct tcaacttgat atgtttgttc ttcatctcca
    22201 cctataatta tatagccatt ctttctacca aattttttac attcactagc attagctaat
    22261 agtatatcta tatgatatgt tccgtttgtt tcaacatata ttcttcctcc attatcttta
    22321 actgtatata ctttgttgtc ataggcagta ccaggaagta taatttttac tttatctcca
    22381 tattcaaaaa ctggatgttt ctttagaaaa tcatcagtat accaagtttt cttaactcct
    22441 tctcgattca ttggaccagc aacagttctt gattttacat caagtggctt tccattgcaa
    22501 tctgtttttc cgccttccat tgcattgttc cctggataat atgcagtaaa tatagcagga
    22561 acttttttac ctgtattttt tttcgtaata ctttgtgcag gaccattttt cttttcatct
    22621 ttattattag tatttccact actatatgag cttgaagaat atgaagcaaa ttcgtctcca
    22681 tcaacaaggg tcaaatccat aaagtgcgaa ttattttcaa atgtatgttt tactttctca
    22741 actaacatat aattttgtaa ttcaatatct cccaaatcta aaaaaacagg tactaaacaa
    22801 cctgctctca ctctaatatc accaagtaca ttttttaaac ttaatgactt agttttctta
    22861 ttatatagtt ttagaagtat atcacacttt tgttttatct ctgcttcact catgtttttg
    22921 tctactgtat caaacatttg aagtattccc caactcctca tatgcgttga gtcttgagca
    22981 atatacacat ctctttttcc tgtttcttca ttatctctca caagtttaat ctttgtgtaa
    23041 gtatcactat ctattgatga attatagtca aaatcttcta ttacatcatt gttcataaca
    23101 gtatctaatt tcattgatgc aacattcttt aatgttattc ttccaaaatc atcatataac
    23161 acatacattt cttttttctc tcttagagta tcatctagtg ctgttagtat catgtcaaag
    23221 agtgttttat tttcttcgac tctagatagt ttatacttag tatcttctat gacattgtat
    23281 tttaaattaa aatctttagc caacattttt acaagttcac ttgctgtttt attgctatac
    23341 acataagtat ctttgttctt aaaatatctt aactggtcgt aagcaacaat tttaatgtga
    23401 ttttctttat ctcttttctt ctgaaatata tatccataga atatacctac acctttgtaa
    23461 tatagtctta cagagttacc ttcacaaaac tctaatatat catccatgac tattgtaaat
    23521 tcaagcttag atggtgttcc tcgtctctct atttcccatg tgataccatc aatgacagca
    23581 ggttcataga aatcttccca atgtgctatg attaatctta catctctatc attcgccaga
    23641 actaaatcat cagccaagtt ttaacacctg ccctttgtaa atagtgtatt tacttaagtt
    23701 tttgccctta tttgccttat ccatcataga tttatttagt tcgtatactt tcttatataa
    23761 tgaaccatta ccaagttgct tcttgcagat taaccaaagg ctatcccctg cttttactgt
    23821 atatgtttta ccatttggct tattggatga atctggtcta aattcttttg gtttcatgac
    23881 tggaggggga gtcctaccat agtttgtctt ttcaggagtt gcaggaacta actttttagt
    23941 tgagtaatct ctatattgct ttaactttat tgcaactttt gtatctgagc cattttctgc
    24001 atcttctact atgttatatt cctcaagaga tactttcata ttagtgttaa atagtacttt
    24061 attacctaat tccctcgata caataaattg aaatggctta caatcagttt ttagtaattc
    24121 tagtttactt aaaaagaatt gaacatctct aaattgacct ctacaaaaag gtaatttatt
    24181 atgtgtaaat tctgcttcaa aacttatttc agataatcct tcttttttta atatatttac
    24241 ttctccaaca tttattaaat caactgtctt gtttttattt gtaactttaa tctctaactt
    24301 tggcggaggt attggtaatt gtactccatc taaataaaag tcataagcca tttatatccc
    24361 tcctctctaa actattccct cagctgatac aaccatagcg tcgtttaatt tttctgttag
    24421 tacatttact attccatcta catctgcctc actatttatg ttgtttgtat tgttcatgtc
    24481 aattttaatg tttactcctg tgaatctatt tattgtttct tgctctgcaa tatctctaag
    24541 atattttaag tcttcttgac ttttatccat tgttttagcc attttggcag tatttcccgc
    24601 cgtgtccttt acattttttc ctaaatctcc accaaatcct ccaagattat taagtggatt
    24661 gtctttatcc cacaaatctt ttaatcctag cttatctttt gcatcttcta gcatcttatt
    24721 aatgtcaaaa gtatctttta atttattaga tatagcattt tgccatttag ttccaagtgc
    24781 atttcctttt tgaaattctg ctccaatatc tttgtatccc attcgctcca attttacttt
    24841 ctctggtgcg tctcctaccc atctgtttaa gctatcaatc tgttgtttga tgtaactatt
    24901 atctgcctta actggcgtaa acgttgcctc tcctacttta ccaatattta tccctggtat
    24961 tttatttatt aggtcaatta acttatttac ccctcttatt gctatatttg ctccatccac
    25021 aaaagcctta ccaagtgcgt ttcctgcatt atttacagaa tcattcaatg atgccatttt
    25081 ctcaattata aaaattacac cttttgcaat tgcttgtttc atcaaatata cacactgatt
    25141 ccatccattc gcaattcctt catttacatc tacacatcta ttaaatagcc atatcattat
    25201 attttgtatt gttgcaactg ctgcaaatac tgcacctaca atcaccccta gaacagtcaa
    25261 agacgtacct gcaaatttat ttattgccgc tactaccata aatattacag ctattactgc
    25321 tataaatcca agaactatcc acacgagcgg gcaagcatac aacgctgcat ttaggccata
    25381 ttgtgctgct gttgcctggg ctgttaccat cgcttctata gtttgtgcag atattttacc
    25441 aaataaagct aattcttgtg ctgccaatgc tgcacaagct ttatatgccc ctaaagcata
    25501 cagggcattt acaaaagatg atactccagt tacaattgca cttgcaagta aaattccttt
    25561 atatattgca atagctgcga caactccata aacagcaggt gcaataatac tccaattttg
    25621 agcaaagaca ttagcaacat ttagtgcagc attagtaatc catcccaatc cttgtattat
    25681 cagtacagtt cctgttgtaa ttccttgtac aaaatctgaa aaaaaatcac tatttaatat
    25741 attttttata atatttaatg tatcataaat gtttgaagat attgcaatca taacattccg
    25801 catagataca acaatctcac taaaacgtac actttgcaaa gctccgctta tcttactgag
    25861 agtctcagaa aatatcatat aagcatcatt tttcatcatt gtgagagctt gagaaaaagt
    25921 gattggcata cttgcaaatt tcttttctat ctcatcagaa gctttaaaca gtgcatttct
    25981 tataacatct gctgttattg ctccgctact tgataattcc ttcaattggt ctttagtttt
    26041 tcccattgca tcagctattt tagtggctaa taagggtgca ttttccatta tggaacggaa
    26101 ctcatcacct tgaagttttc ctgcacccat ggcttgtgtt aattgataca ttgctgcact
    26161 agcttcattt gctgatgttc ctccaattac aaaagcttta ttcataagtt ctgtaaactt
    26221 tatcacctct gctgaacttc caaaagcatc tcctgcaagt attccaagtt tagcaatctg
    26281 tgctgctgta tctgcataac tagctcttgc actttgggct gacaaataaa tcattttatt
    26341 aagctggtct gttgtctgta agccatcatt cattagattt aaacgtgctt ttgtacttgc
    26401 aattgtatct gctgcttttg taatactttc tattcctttt attccaatgt atatcccagc
    26461 aatacttttt aatttagaca ctaatagaga acctgcttca ctaccttgcc ttattttatt
    26521 attaaagttt tcctgctcat ttgtatttct tccaattttc tgttctatcc ttgtgagaat
    26581 actttctatg ttattcaaac tttgttgaga agcttgtata ttacctgcat tgagtggatt
    26641 attcaatctg ccttgcaatc tctctaaact attaattgtt gtattaatag aattagtcat
    26701 attacgaaat gctggtgtca ttccgtcgaa aatctttatt gaagtttgta ttgtagccat
    26761 ttcctcactc tcctttcata aatttccata taaaaaaaca cctacattag taagtgttta
    26821 ttatttatgc ccaaaactgt ttaccacagc tcaaacatgt aactctaact ttctttgctc
    26881 ctaaatttcc agctactaaa ccaatgcctc ctgctatagt agcccctgct acagcttttc
    26941 ctatgccaaa acctttctta tgagcagaca atgaaacaga gccacaccta gggcaacgag
    27001 caacatcatc ttcaagtaca ttagtttgtt tatactcctc ttccctcaat atctcttttt
    27061 cctcttcgat atttattttt tcacttaatt ctttaaaagc tggatatata atatttttac
    27121 actcttttat gtccagccct agtaacttca ttgcaatatc aattgctaag attttatctc
    27181 ctttagtttc tttataaata ttagtcagat ttaattcttt tccatgaaca tttataaata
    27241 aatcatcatt gttaacttta tctgctaatg tagctgttcc gcaatttgag caaaatttgc
    27301 ctgcaccagt tatctcataa ccacaatttg aacaaaacac aacaatcacc tcacataata
    27361 cttattttat agcactatta tactatatac gtaaaatttt tacattatta tcacctcctt
    27421 tcataaaaaa aaacacttac tcatttgtaa gtgtttttga attattttta attttaagtc
    27481 cacatagtta atataaaacg agcatcattt atcattgcta atcgtgcatt tgtacattta
    27541 cataccacat agttaatata aaaccataca ctagattcta ccccaaaaat gaagctcaat
    27601 atatttacat accacttagt taatataaaa cgcgttaatg cagtaccata acgatatggg
    27661 actaactaat ttacatacca catagttaat ataaaaccgt ataggaatga atgcaggtag
    27721 tgcattttct agtacattta cataccacat agttaatata aaacgcaacc cctaaagacc
    27781 ttctaaaaaa attactctta gatttacata ccacatagtt aatataaaac acaggtgtat
    27841 ttacctatag aacaacaaga gttacattta cataccactt agttaatata aaacattcaa
    27901 gaagtaactg taattgttca tctgtaaaat aatttacatt ccatatagtt aatctaaaac
    27961 ctggtcttaa aaacatacat gttgcaaaag tgaatgatga atttacattc catatagtta
    28021 atctaaaacg aatgatacat tagaatatat attttgtagt gaatgattta cataccactt
    28081 agttaatata aaaccatcct acttagatac tgcacaaata gtatcgcgaa tctttacata
    28141 ccacttagtt aatataaaat tgaccagtac gaatgcgagg ctagcaatga taaacgactt
    28201 tacataccac attgttaata taaaaacctt gagtgacgca gcaaaagggg caaaggatac
    28261 agcatttaca taccacttag ttaatataac tcctacctat attatatcat tttttactac
    28321 ataaagcact tgaaacagta aaatattcaa gtgctttatt tgtctattta tttttctctt
    28381 tttttcctac attcttcttt cactaaactt acaaatctat aaaattcatc tggattttca
    28441 tttttcattt tttcaaagat atttccaagt tctttgataa gttctattct atctatatca
    28501 agtaaattct catgcattgt attaatcccc ccaaaactaa actaaaatat ttaatacaac
    28561 tgataaattt actcaatttt ataaaccaca tgataattct tcttctcacc tgcaatcttg
    28621 gtaggtctat tattctcctc tatccaattt cttattttat ctattacact ttctgtatac
    28681 ttatttacag taccagtcca acttccatta gtctcccaaa ctcccttaac ttcactatct
    28741 tctaaatcaa tctttttaat aatctcacaa acagccatct gagctggttt attactctta
    28801 gaatatagtt ttaacttaga tgctatttgt tttgtgtcaa aataatgttc ttcttcgttt
    28861 atctctattg gcaaatcaat tcctgccttt ttatataatg ttttagctgt aagtaactta
    28921 gatttattgt caaaacctgc accatccaat agttctttta acatagatgt gctgttataa
    28981 gccagttgta atttttcaat ctcacttgct ttttctctta gtttttcggg gtcagcatta
    29041 tttgttatgt atgcaccagt ttgtcgaatg gctggaagta cttcatcact tatccaatct
    29101 tgaaatctct cagcttcttc ttttttagat ttaaatataa gtttataaac accactttca
    29161 gttagaaaat tttctcctgt attatgcaat tttctaaact gcatattatg cattttagaa
    29221 ttagtcaact taactacttg attatcatta aattttctaa tactactgtt cacatctgaa
    29281 atatctaaac actctgccac atgctttgga ttaaataaaa tttgcccttc aaattcgaat
    29341 acctctatat cttttccttc aaaattcatt aattcattca taataaattc ctccttaaat
    29401 ttgattgtaa gaagtacctt actatgatag aatatatttc atagaaggta acttctttgg
    29461 gaaacagtcg caagtgcttt ggtcggtgca gcggctgttt tttatttgtt tttgtcaatc
    29521 ttctctttca ccaattcaat tcctctaatt acaacatctg ttttagatat tttaagattt
    29581 tcagcacact catttaatat atctgcttgt tcttggttaa gtctaacttc aaatcttaat
    29641 tttttggaat tttcttttgg tggtctgcct aatttattgg acatcttatc acctctcttt
    29701 ttattgtccg tacttaaatt ataatatagt acgtacaata attcaagagt tttttactaa
    29761 ttttttctaa ttattttact caaccgacca attttgagca aaacaaaagc acctaccaaa
    29821 aagtaagtgc tttctatata acagatttaa ttttaaaata aagtattcac ctaatttcca
    29881 gacgagaaat ccaaatcatc aattaaattt acattatttt ttatattatt ccaatcatct
    29941 aaaaatacat tataagtgag tgtcacatca acaataaagc ttattctcac tgaatggata
    30001 tcattcatat cttttatatt gtttacaaat tctttagtaa agtttctaca ctcattttca
    30061 tcattaaacc tagaattttc cacttgaata ctaactatat atccctcacc ttttgttggg
    30121 tttaatatgt taacataata tgttgtttta tcttttaagt cttctggtat catagagtct
    30181 acttttgctt gcatctcttg tttataaata tcttcttttt ctctttcgct aattatattt
    30241 tcttttgtaa tatttccttc atttacttca tctctatttt ttgaatctcg tatcgcttga
    30301 tgaatcatca ttgatactgt aaatacaaca aagtatatta aaaatatcac taaaattttc
    30361 ttcaacaaac ttaatttttt aaattttccc cacataatga actctcccct attaaattct
    30421 ttattcaaat ataattctat ctaaaaataa gaacttagtc tattgattta aaatattttg
    30481 agcaaaataa aagcacctac caaaaataag tgcttctttc ttctatttag tttttctcca
    30541 cattgttaat ataaaagttt ggagttttaa aattatcatt cctatctttt gctttattta
    30601 cataccacat agttaatata aaattgaggt agaaaatgat aaaaagactt ttagagtaat
    30661 ctttacatac cacatagtta atataaaacc tgttaatgta atcatttcac catcataagt
    30721 tgcgaaattt acattccata tagttaatct aaaacaataa ttggtgatgg tacaggatat
    30781 acaaatgcaa catttacatt ccatatagtt aatctaaaac agaaaattca ttgtacaatt
    30841 cttctttgta ttttttaatt tacattccat atagttaatc taaaacccca aaataaactt
    30901 agtatttcca atatctacac atacacacct ctctcaaatt tgcagtgaac catgagtagt
    30961 gcaattgata acatttatca cacaccctca ataccttgta ttccaattgt taaacttcaa
    31021 tttatcgcaa atattgctca ctgcaaaatc tctacatttt tattatatca taaaaatatt
    31081 atttttgaat atctgtacca atttgtggta taataaaagc aaggaaataa tttactttat
    31141 acaagagtag ctatttccat caaaattgat ttaaagaatt atttttttaa atcaccctta
    31201 ttggcgtctg ggtgattttt tattttgtca taaatataag ctgatataac accagctagt
    31261 atgcttaata aaaaaactat catataattt cacctccttc cttatttgga atttggcgtt
    31321 taatatgaaa ataatcaccc ttcgcacttt cgattattat ccttgctaca a
//
LOCUS       DAYATN010000034        31110 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.034, whole genome shotgun sequence.
ACCESSION   DAYATN010000034 DAYATN010000000
VERSION     DAYATN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.034"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            82..528
                     /gene="bilQ"
                     /locus_tag="ACGI8A_001514"
     CDS             82..528
                     /gene="bilQ"
                     /locus_tag="ACGI8A_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin utilization transcriptional regulator
                     BilQ"
                     /protein_id="HFH0179516.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            530..1624
                     /gene="bilR"
                     /locus_tag="ACGI8A_001515"
     CDS             530..1624
                     /gene="bilR"
                     /locus_tag="ACGI8A_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin reductase"
                     /protein_id="HFH0179517.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1712..2242
                     /gene="bilS"
                     /locus_tag="ACGI8A_001516"
     CDS             1712..2242
                     /gene="bilS"
                     /locus_tag="ACGI8A_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein BilS"
                     /protein_id="HFH0179518.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            2593..2928
                     /locus_tag="ACGI8A_001517"
     CDS             2593..2928
                     /locus_tag="ACGI8A_001517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179519.1"
                     /translation="MPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWL
                     QIHAFYSDTIADYLTNFMCFVALSIEIFSIFFIYNIVRHVTIIIFIDILVFIALLIIG
                     VYKLFKKYN"
     gene            complement(3178..4554)
                     /locus_tag="ACGI8A_001518"
     CDS             complement(3178..4554)
                     /locus_tag="ACGI8A_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179520.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDEKDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDSIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            4822..5994
                     /locus_tag="ACGI8A_001519"
     CDS             4822..5994
                     /locus_tag="ACGI8A_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0179521.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYAIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            6008..6655
                     /locus_tag="ACGI8A_001520"
     CDS             6008..6655
                     /locus_tag="ACGI8A_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFH0179522.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6657..7166
                     /locus_tag="ACGI8A_001521"
     CDS             6657..7166
                     /locus_tag="ACGI8A_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFH0179523.1"
                     /translation="MSQQNTGNVIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7530..8642)
                     /locus_tag="ACGI8A_001522"
     CDS             complement(7530..8642)
                     /locus_tag="ACGI8A_001522"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFH0179524.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            9006..9206
                     /locus_tag="ACGI8A_001523"
     CDS             9006..9206
                     /locus_tag="ACGI8A_001523"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFH0179525.1"
                     /translation="MVGGGDIVKLFMKDNLIDDYYIYILTIILGRGISLFKSGIDKTN
                     LNFKKASNIGELVKLEYSKKQK"
     gene            9451..11178
                     /locus_tag="ACGI8A_001524"
     CDS             9451..11178
                     /locus_tag="ACGI8A_001524"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179526.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11178..12968
                     /locus_tag="ACGI8A_001525"
     CDS             11178..12968
                     /locus_tag="ACGI8A_001525"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179527.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13130..13438)
                     /locus_tag="ACGI8A_001526"
     CDS             complement(13130..13438)
                     /locus_tag="ACGI8A_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179528.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(13700..14248)
                     /locus_tag="ACGI8A_001527"
     CDS             complement(13700..14248)
                     /locus_tag="ACGI8A_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179529.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(14351..15022)
                     /locus_tag="ACGI8A_001528"
     CDS             complement(14351..15022)
                     /locus_tag="ACGI8A_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0179530.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            15415..16323
                     /gene="garR"
                     /locus_tag="ACGI8A_001529"
                     /pseudo
     CDS             15415..16323
                     /gene="garR"
                     /locus_tag="ACGI8A_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
     gene            16459..17841
                     /locus_tag="ACGI8A_001530"
     CDS             16459..17841
                     /locus_tag="ACGI8A_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HFH0179531.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            17912..19348
                     /locus_tag="ACGI8A_001531"
     CDS             17912..19348
                     /locus_tag="ACGI8A_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFH0179532.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNGGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(19534..20388)
                     /locus_tag="ACGI8A_001532"
     CDS             complement(19534..20388)
                     /locus_tag="ACGI8A_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HFH0179533.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(20656..20979)
                     /locus_tag="ACGI8A_001533"
     CDS             complement(20656..20979)
                     /locus_tag="ACGI8A_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HFH0179534.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNKNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21182..21811
                     /locus_tag="ACGI8A_001534"
     CDS             21182..21811
                     /locus_tag="ACGI8A_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179535.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            22123..22632
                     /gene="ruvC"
                     /locus_tag="ACGI8A_001535"
     CDS             22123..22632
                     /gene="ruvC"
                     /locus_tag="ACGI8A_001535"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFH0179536.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            22625..23227
                     /gene="ruvA"
                     /locus_tag="ACGI8A_001536"
     CDS             22625..23227
                     /gene="ruvA"
                     /locus_tag="ACGI8A_001536"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFH0179537.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            23334..24353
                     /gene="ruvB"
                     /locus_tag="ACGI8A_001537"
     CDS             23334..24353
                     /gene="ruvB"
                     /locus_tag="ACGI8A_001537"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFH0179538.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            24671..25696
                     /gene="queA"
                     /locus_tag="ACGI8A_001538"
     CDS             24671..25696
                     /gene="queA"
                     /locus_tag="ACGI8A_001538"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFH0179539.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            25708..26193
                     /locus_tag="ACGI8A_001539"
     CDS             25708..26193
                     /locus_tag="ACGI8A_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HFH0179540.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            26272..27393
                     /gene="tgt"
                     /locus_tag="ACGI8A_001540"
     CDS             26272..27393
                     /gene="tgt"
                     /locus_tag="ACGI8A_001540"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFH0179541.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            27527..27820
                     /gene="yajC"
                     /locus_tag="ACGI8A_001541"
     CDS             27527..27820
                     /gene="yajC"
                     /locus_tag="ACGI8A_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFH0179542.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            27878..28240
                     /locus_tag="ACGI8A_001542"
     CDS             27878..28240
                     /locus_tag="ACGI8A_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HFH0179543.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            28306..28449
                     /gene="scfA"
                     /locus_tag="ACGI8A_001543"
     CDS             28306..28449
                     /gene="scfA"
                     /locus_tag="ACGI8A_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HFH0179544.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            29075..30970
                     /locus_tag="ACGI8A_001544"
     CDS             29075..30970
                     /locus_tag="ACGI8A_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HFH0179545.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKGGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT        11915 a         3548 c         5321 g        10326 t
ORIGIN      
        1 atattcttga ctatattaat tgtttaatat aatatagttg atatagtcaa acatattata
       61 attatagaag ggaaatttaa gatggatttt aaaaatttac agtatgaaag tttgtcatat
      121 attatttgta acttgcaaaa aaactttaaa ctttattgtg aaaaatgttt aaaaccatat
      181 aaacttacga atggtctata tttctattta atttatatta ataaaaatag gaattgttct
      241 ttgaatgatg tttctacaga atttgaagtt gataaagctc atacaacaag aactatatct
      301 aggttagaac aagatggata catagaaaaa atccaaaacc ctaatgatag tagagctttt
      361 cagttgagag taacagataa gggtgaggaa gtattagggg atataaaaaa catattttca
      421 aagtgggata atcacataaa aaaagagttc tcggatacag aatataaaga actggtaaag
      481 aatttacatg tggtaaagga tataaaaaca gctgtggagg aagaatagga tgtattgtaa
      541 tttattaaaa ccaattaaaa ttggaaattt ggaattaaaa aatagagtat cttttgcacc
      601 tacaagcatg ggtcttaaat tggaagaaaa aattaaaaag ttttctgata ttgctaagtc
      661 aggagtagct ctgattactc taggagatgt atctattaga cctagttttc ataaggtagc
      721 tataagccta tcggatgaag atggagtcat gaaatacaag aaaattgtag atgaaataca
      781 caattctggt gcaaaggtat cagcacaact attttgttca gattatgatg ttaatttaat
      841 taaagatact atgaatatgg gaattacttc acatgatgag ataaaaaaaa taatgaatga
      901 cggagtaaaa gattacataa caaatatgcc caaagaagaa attaaaaata ttataaattt
      961 atttaaagta acagcattga atgcaaaaaa agcaggtttt gacatgattc aaattcatgg
     1021 tgataggctt gtaggaagtt tttcatcaag tatatttaat aatagaaatg atgaatatgg
     1081 tagaacgtgt gataatagaa gtagatttgc aagcgaaata attagtagta tccgagatga
     1141 agttaaggac attccaatag actataaatt tgcaataaga caagaaaatc ctcattatgg
     1201 aaatgcagga gttttacttt cggaagttga gtattttgtt aaaaaatttg aatccctagg
     1261 tgtaaacagt tttcatgtaa cacttgcaaa tcattcaaaa ttagaagata ctataccaac
     1321 caataatcat ccttacttta aagaagaagg ctgtttttta tatttagctg atgaagtaaa
     1381 gaaacacaca aatcttccag tatgtggggt tggaaaattg tctagcccag attttataga
     1441 aagtattatt tcaaataata gagtagatat ggtttctatg tcaagacaat tactagcaga
     1501 tagtaattgg ttacagaaag taaaagatgg aagagtagat gagattaaaa agtgctgtta
     1561 ttgtaataag aagtgtgcag atgctttaca aacacgctct cagtttggat gtatactaga
     1621 ttagaatatc cttataaaaa taaataattt gttaaggtta atatattttt ggtgatttag
     1681 ataaatattt agataaagag gaggagacat catgaaaagt ttagttttat attcaagttt
     1741 aactggaaat actaaaaaga ttgcttatac tatatatgat gaaattcaag aagaaaaaga
     1801 cattaaagat gtaaatgaat tagttgatta tggtattgac tatgaaaatt atgatatagt
     1861 attcttaggc tattgggtgg acaagggaat ttgtgataaa aattcaaaac aagtgcttga
     1921 aaatattcac aataaaaaga tagcactatt tggaacaatg ggagcaagta aaaaaggaag
     1981 ttatggagct agtataatag aaaagataga atcaattatc ccaaaagata atgaaatact
     2041 aggttcattc atatgtcaag gtaagatagc agaaggattg aaagcaaaat ataaagagat
     2101 gttaaaactt tctcctgaca acgaacatat tcgccaacaa ttaaataacc atgaggaatc
     2161 tcaaagtcat ccagatgaac aagaaatata tgaagccagt atgtttgcaa aaaatatgat
     2221 gattaaagct agtattgtgt agtagttaaa caataagttc atataagata tagaataaat
     2281 aatttagtaa aaaagtatag caaataattt gctatgcttt ttttatgaaa tcaaaaaaat
     2341 aataaaaata cttgactgta aaccttgttt atacattatt ttatagaaaa taagatagtt
     2401 ttaaatataa aaaaagatat attattgaaa ggagatatta ttgtatgaaa acttataaaa
     2461 gataattagt ttgattttaa gatatactaa atatacaatt ttatagagtg aaaatattta
     2521 agactatatt atagttataa attaatagaa tagagaggta gtgtttaaat tgcaataaca
     2581 gtatttcaaa agttgccacc tcaaattcct atccaatgga ataatggtgt agctaattat
     2641 gtagataaaa aatttattta tgcctaccct gtcatttgtg ttgtattcag aatactttta
     2701 cgtccattta tatggagatg gttacaaata catgcttttt atagtgatac tattgcagat
     2761 tatttaacga attttatgtg ttttgtagcg ttgtctatag aaatattctc tatatttttt
     2821 atttacaata ttgtcagaca tgttacaata attattttta tagatatact tgtatttatt
     2881 gctttactga taataggtgt gtataaatta tttaaaaaat acaattaatt tttaaaaata
     2941 agtaataagt gatagtgtaa aaagtagcca ctgcaaatta tggattgtag tggctatttt
     3001 attatttgta tatgaaatta ttttgttttt ctaataagtt acgatatatt atggtttatt
     3061 tcactgataa aaggtttagg tattttaaag taaaaagttg tatattctaa taaaatttaa
     3121 acattttgat tctttaaaat tagagaaaat acattgcttt tattaaaaaa ttaaatatta
     3181 agaatttaat ttctgcatct tatttagaaa agataagaaa tcaactttat atattttttt
     3241 tggccttcct ctttgattat cgagttttgt atttacaata gttgcaattc ctgctttata
     3301 taagatactc aatattctat tagcacttct aagagttatg ttcatatagt ctgctaatat
     3361 ttctgctgac acattatttg aatttctatc attaatcata cacatgattt tagatacatt
     3421 tgtattagat agaggtataa aagaagcaac tttattggct attgagctat cttcaatatt
     3481 attagaattt ttatttgaat ataaagggcc tattgtatca ttagtatcat ttactataaa
     3541 agtacagttt ccattcaaag aagcagattt accattagct tgacgagcat tttgctctgc
     3601 atgaatattt gtatttccaa tgccccaacc tatatttaag ctatatttaa tttcttttaa
     3661 atcttctgaa ataaaaaaat ctgtatagtt attggtcata ttgtttaaca tttctttagt
     3721 agtgtgtatc tctattttca tatcattttt tttgacctga actccataga atccatgtct
     3781 tgatagagaa tttaatatag tgttatatat ttgattttgt atctcatcat ttttaaatat
     3841 caaatcttca tttatgtact ctgaactatt tactgtaact atacaagttg cagatttatt
     3901 tttgtctaag ttttgtaatg ttatttcatt caccagagat ttaaatgtat tcaggattga
     3961 ttctgtagat ggaaccatgt aaatagaatc atatccaagt ttattaagtt cacaactaat
     4021 gatgtaattt ctagtaaaag aaaggttaat cttattatct cgcaataagt ctaaatgtat
     4081 ttccaataat ttatcattta attttttaaa gtcatataca ggttgaggga tattaaaatc
     4141 tttaattgta tatggacatt tattagagtc tattaatgaa tatatatcat aataattgtt
     4201 gtcctctctt aaaaaatcca taaatgattt tgtaaagtca atatcttttt ggctatttaa
     4261 taatttgaat aagtatttat aaaaatcttt ttcatcaagc tttaaaaaat ttaatggaac
     4321 ttttatgtct tttatattta ttataagttc actatatgga actataccac tgcatacaaa
     4381 tccatcaaaa ttatctactt gctcatagta tatattaggt aaattattaa aaggattctc
     4441 ccctttaagc tcaataaatg taatttcaca agtatcattt aattctttaa gagcttgttt
     4501 aaacttatct gacataaaat aagatgttgt caaaaaacca attttatatt tcataaaacc
     4561 tcctttaaaa tttcgagagt attgtaacta atcaatcaat ttataacctc attttataat
     4621 agtagataaa acgcgtcaat ttattttaca aaaggaaaat tgaaagaaaa atcagaaaat
     4681 ataaaaaaga ctgaaaaata tgttgactgt caagaataat tgtgctattt tgtaattaag
     4741 gactagtctt ttaagatttc ctaaaataaa cttagatttt aggaaagaaa tacattttat
     4801 ttgaaaataa aggagaagat tatgaataca tatttaaatg aaatttctaa gtttgttgat
     4861 gagaatagag aagaaatagt ttcattatgg aaagagattg ttaatatgga aagctataca
     4921 cattgtaaag aaagcgtaaa taaattggct aagagattaa agtttgagtt tgaaaaagaa
     4981 ggtttagatt gcgagttagt agatgttgga gataatggta gtacattgat aggtacactt
     5041 ggttccaata tagataaaaa gccaataatt ttttcaggtc acatggatac tgtctttgaa
     5101 acaggtacat ttggagagaa tccatttaaa ataatagaag gaaaagcata tggcccaggt
     5161 gttttagata tgaagggtgg tataataatt tccttatatg ctattaaagc attaaataaa
     5221 ataggataca aagaaagacc aataaaaata gtattttctg gagatgaaga gataggacat
     5281 aaggattcaa caggtgcaga tgttatattg agggaagcaa aaggtggtct ttgtgctttt
     5341 aatatggaaa ctggattagt agataatagt ttgtgtgtag gtagaaaagg aagaatagga
     5401 tgtaatatac atgtaaaagg tgtagaaaca cacgcaggta atgattttga aggtggaaga
     5461 aatgctatag aagaaatggc taataagata ctacgcatac aaaaattgac taatttagat
     5521 gttggaacta cagtaagtgt aagtgtgatt aagggtggaa gagtggaaaa ttctattcca
     5581 gaagattgta gtataaaagt agaccttaga tttgaaaaag tagaagaaat ggagaatgtt
     5641 aaagaacaag ttagagagat atgtaaagaa acttacatag aaggaacttc tacagaagtt
     5701 aattttataa gtgaaatgat gccatttgaa actacagaag atgttatgag atttcataca
     5761 tttgtaaatg aagtgtcaaa agaaaatgga tttggagaat taaatgctaa gagacttgga
     5821 ggtagttcag atgcaagtta tttaactatt gcaaacgttc ctacaatctg ttcatttggt
     5881 gtaagaggag aatggaacca cacatcaaga gaatatgcag tagtagattc tatgtttgag
     5941 agggtgaaat taatttctac agttatttta aacttagata aatttgaggt ataaaggggg
     6001 attttatatg gatgtaacag ctggagataa taaggtaaca tggaaagggt atttatcttt
     6061 atttgtattg atagtattat tttcaggagt gtttaaagat agtcaaggct tccttaaagc
     6121 attagatttt gctaatctga caggttcatt tggtactatt gcagatggtg tagactttat
     6181 gggtaaagga ggaacgggag caaaagatgg tttcttattt gctctgactt tgattccaac
     6241 tgtctgtttt gctgtaggtc ttatagatgt agttgaatct atgggaggaa tgaaagcggc
     6301 atcaaagata tttaatccat tgcttagacc tctgcttggt atacctggaa tagcaggaat
     6361 agcatttgta tcttcattta ctagttcaga tgtagcctca ataatgacga aagaattatt
     6421 tgaaagtggg gaaataacag atgatgaaag aactatattt gtagcatatc aatatgctgg
     6481 ttcagctgtt atactgaaca caataaatac tcaagctcca cttttaccaa tagcattatt
     6541 ggcattaggt ccaataatac ttatagagat attctgtaaa atacttggtg ctaatctagt
     6601 tagattaatc ataggtgtaa gaaataaaaa gaataaggct aaggaggctg tgtagtatga
     6661 gtcaacaaaa tactggaaat gtaattgtaa atagtaaacc taagaaaact gctgttgaga
     6721 tatttatggg gggagcaaaa aaaggttttt acataggtgt tgaacagata ttaccagcca
     6781 tgataatggg ttatgtaata gtgcagtttt tacaattaac tggtttagtt gatattttag
     6841 gaaatatttt tggaccagta atgggtattt ttggactacc tggggaatct gttgtagttt
     6901 tgatagcagc attctttgct aaggcagcag gagcagctac agctgcaaac ttatttgcac
     6961 aaggtctaat cactgcacca caagcaacca tattaattat gccatgtatg ttaatgggta
     7021 ctctagttgg tcattatgcc agaatagtgc ttgtagcaaa tgccagtact aagtatagaa
     7081 tgttactgtt ggcagttcct atatttgatg caatagtggg gatgttgata gtaagagttt
     7141 tactcactgt tatgggatta atgtaaataa cataaaatta taacctaact ataaatgata
     7201 tataagaatt aatatataca aggattaatg agtagtaatt gatatatagt aataaactaa
     7261 gatagtaatt aatgtgtata ttaattaaaa aaagagagta tctcaaagtg aactttttag
     7321 ttcatgagat actctttttg tatgaaatca agtatatttt cataatttca acaatgaaag
     7381 agaggcttat ctctattttg agacagcctc ttcttttaaa tttatatttc tagaatttat
     7441 gttttttatt tatccaacat taacattagt tattagcaat aattttaaga aaaatatact
     7501 ttcatgttaa tatcagaata aatgtgtttt taaaggatga tttgttttga attttcacga
     7561 atatctactt ctatgacttt ttctattgta tctcctctaa aacctaataa attacttgtc
     7621 aagtttgcag tggaacaaga atgattaggt ataattctta ttctatcacc aatttttata
     7681 tcagtttcac catttacttt aatttttcca acttcttctg aaagtgaatc aatagtaagt
     7741 tctggatgtc cacataccct accataacca acaatactac tatttccatg tgcaccttta
     7801 tcaagtccaa ggcacttact tccacaatca attaaatagt atccactgct attttttgag
     7861 attacagtgc acacaacagt aagagcacac atatcttcag tagcacaacc taaacctatc
     7921 tgtatagcat catagtatac atagtttcca ggtcttacag cagttatagt tttagaagca
     7981 gcttcagatt caactgttgg agtactacca gaagcgacaa tttctatatt aaatccatta
     8041 tcagttagag ctttgtatgc tttctccata gaagaatctt cttgaacaca aacatctgga
     8101 acaccttctg gagaactaca accatacact tgacctggat gcgaacttat accaacaatc
     8161 ttgatgtttt ctagcttaga aatttcagta gcaagttcaa ccactttttc tggttcaacc
     8221 ccaaaacggt gtaagccaca atctacaata ataagtagct tgcactctat ttttgattta
     8281 gaaaaagctt cactataagc cttagcacat tctatattat ctgtagcaca gattaaattt
     8341 atttttgatg ccatagtcac aattctattt atatttactt ctcccatgta aggataagcc
     8401 aacataatat cttcaaaacc tgcttttatc agagcttcac cttcatcaag tgttccaact
     8461 agaaaaccac tagcacctgc atttttttga agtctcgcta tgtaagtact tttatgtgtc
     8521 ttaagcatag gccaaagttg tttattatta tttgtacaaa tttgttgaat tttatctata
     8581 ttcttttcta actggtctaa atctagcaaa aaatttggag tagcgacttc tgaaattttc
     8641 atgttattta ccacctttca acaaaagtaa attaactata agtattataa ttattatatt
     8701 agtaatatcg gctttataat attaatagct tataattata ttatagaact agcgttaaca
     8761 gagagcaata tttatttgta ataattagaa ttttcaataa gttagtgtag ttttaaatta
     8821 tttatataat atatttttaa ttttatacaa agtttattta aattagtttg aggatttatt
     8881 aatagggtat tattgaatga gtaaaaaagt gatgtttaca tagaataatc acactataaa
     8941 ttaatttaga acctaaaaaa ttaattgagc aaattggaaa agagaaggat aaaaaagata
     9001 tttggatagt tggtggaggc gatatagtaa aattatttat gaaagataac ttaattgatg
     9061 attattatat atatatattg acaataatat tggggagagg gatttcacta tttaagtctg
     9121 gtatagataa gactaatttg aattttaaga aagctagtaa tattggagaa cttgtaaaat
     9181 tggaatacag taaaaaacaa aaataaatta catgccaatt tgttacaatg ttatgaaacg
     9241 aagcatttaa aagacttttg gctatatgaa ttgacattac ttgtcaaata tgttaacata
     9301 taagcgactt gtcggagggt gtattaaaaa gaatatgctg gagaagagag tgcgttaact
     9361 cttttctcct ttttttataa aattaagcat ttataaattt agtattatta catgattaca
     9421 ctatctcaaa aattaagaaa ggagaaaaat atgaaattaa ttttaagtta tttaaagaat
     9481 tataagttat taatagtatt aaacatactg gctatattta gttttgcatt agtcgagttg
     9541 gggattccaa ctataatagc taaaataatt gacaatggta ttgcaaatca gaatatagct
     9601 tacataaaac aaatgggaat agtgattgtt attatttcta ttataggggt tgtaggtagt
     9661 atattgttgg gttattgttc tgctaaggta tcaacaagtg taaccagaga tataagaaat
     9721 gacatatttg agaaatcaca agaattttca catactgaat acaataaatt tggaatttct
     9781 tcaatgatta caagaacaac gaatgatgca tttcaaattc aacaatttgt taatatacta
     9841 ctaaggactg ccttattaac tccagttatg ttcattataa gtataataat gacaattaga
     9901 acaagtgtgg aactttctat agtattggct atatcagtac cttttataat tattggagta
     9961 gcaataatag caaaagtatc tcaggcaata tcttcaaaac aacaaaaagg actagataaa
    10021 ttaaacctta tatcgagaga aaatttaact ggaataagag taattagagc ttttggaaat
    10081 gatgattatg aaacagagcg atttgaaaaa acaaatacat attatgctaa tgtttctaag
    10141 aaactattta agcttatgtc aattactcaa ccagcattct ttttactatt aaatatagct
    10201 gttttggctg tattttggat ttcaagtgag aagataaata ttggagagct tcaagttgga
    10261 cagttggttg catttttgga atatcttttc catgccatgt tttcaattat gcttttttca
    10321 atggtattta ttatgtatcc aagagcagag gtatctgcaa atagaataaa agaactatta
    10381 aatgaagaac cattgataaa aaatcctgaa aatggaatta aggatactga aaataagggt
    10441 atcatagaat ttgataatgt aacttttacc tatccagatg gagaagcttc tgtacttaag
    10501 gatatttcat ttactgcaaa aacaggtgaa acagtcgctt ttataggaag tactggtagt
    10561 gggaaaagta ctctaattaa cttgatacct agattttatg atgtaacaga aggtagcatt
    10621 aagataaatg gagtagatat aagagaatat gatttaaaag ctttaagaaa aaagatagga
    10681 ttcatacctc aaaaatcact gctttttact ggtagcatag caaataatat tagatttggg
    10741 aaacataagg caggagagtc agaattagaa tattccgcaa aagttgcaca agcatatgag
    10801 tttatatcaa aaaaacctag aaagtttgat gaattgatta gtgaaggtgg agcaaatgta
    10861 tcaggaggtc aaaaacaaag actttctatt gcaagggcta ttataagaag acctgaaata
    10921 tacatttttg atgatagttt ttctgcactt gactttaaaa ctgatgcaat acttcgtgct
    10981 aaattgaaga aggaaacaaa agatgcaatt gtattgattg ttgcccagag aattagttct
    11041 attattgatg ctgataagat tatagtactc aatgaaggac aagttgtagg aatgggaact
    11101 cataaagaat tattaaaaaa ctgtgaaata tactatgaaa ttgcaacatc tcagttaaaa
    11161 aaggaggaat tagaataatg aaaaagtcta aaggttttaa gaaaacaatt ggtagacttc
    11221 ttccatttgt aaagaaatat aaattttcct ttattatagc tattatatgt ataatttcag
    11281 cagctactat gaatgcactt gcacctaaaa ctgaaggatt gataataaca caattgacta
    11341 aagatgtaat tagtatcgca aaaggagtac caggggcttc cgtaaatttt gattatgtaa
    11401 caaaaatatt ggtgatttta gcatgtatat attttgccaa tgcaattttt acgtatactt
    11461 caagttttct acttacgaat gctattcaaa acacaatgag agatttaaga aatgaagtcg
    11521 aaaataaaat aagaagattg cctattagtt attttgatag taatagtttt ggagatgtat
    11581 taagtcgtat atcaaatgat gtagatacaa tatcaaatgc attgcagcag agttttatgc
    11641 aaattgtaaa ctcaatctta gtcataattt tggcactttc aatgatgttt acaattaata
    11701 tatatatggc tcttatagct ctttttataa ttccaattag ttattttgta tctaaatttg
    11761 tagtaaaaaa atctcaaagt agattttcat tgcaacaaaa tgctcttgga aaattaaatg
    11821 gaaaagtaca agaaatgtat actggattta atgaaataaa actatatggt aaagaagaag
    11881 attctataaa agaatttaaa aaagtaaatc aagaactttg tgaaaatgga tttaaggcac
    11941 aatttatatc aagtatgatg aatcctatgg tttcattagt aacttacttt ggaattgcag
    12001 ctgtggcagt tgtcggttct atatatgcag taagtggagg aataacagta ggaaatttac
    12061 aagcatttgt gcgttatata tggcagataa atcagccact ttcacaaatg actcagttat
    12121 ctacagtaat acaatcttct tttgcagcta tagaacgtgt atttgaaatt ttagacgaag
    12181 aagaggaaat acctgatgtt gaaaatccag ttaagataga aaatgtaaaa ggaaatgtta
    12241 catttgagca tgttaatttt ggatatggag aaaattcaac tttaatagaa gatttaaatg
    12301 cagaagtaaa aagtggtcag atggtagcta ttgttggacc tactggagca ggtaaaacaa
    12361 cattaataaa cttattgatg aggttttatg atgtaaagaa aggtgctatt aaaattgatg
    12421 gagtagatat aagagatatg aaacgtaaag atttgcgttc aatgtttggt atggtgcttc
    12481 aagatacatg gttatttaat ggaaccattt ttgaaaatat agaatatggt agatttgggg
    12541 ctactaaaga agaaattata caagcagcaa aagtagcaaa tgttcatcac tttataacca
    12601 ctcttccaga tggatacaat atgtttttaa atgaagaagc atctaatata tctctagggg
    12661 aaaaacagct tttaaccata gctagagctt ttatatcaga cccttcaata ttgatacttg
    12721 atgaagctac gagttctgtt gatactaggc tggaacttat gcttcaaaaa gcaatgagaa
    12781 atcttatgaa tggtagaact agttttgtta tagcacatag actttctaca attcgcaatg
    12841 ctgacttgat tttagttatg aataatggaa gtattataga gcaggggaat catgatgaat
    12901 taatggaaaa gggtggattt tatgagaagc tttataatag ccaatttgca gacaaagaat
    12961 cagaatagat aacataaact tttgtataaa attttgtatc aaattcaaat attaatcata
    13021 taattaaaaa gagtatcagt aagttgtttt tttatagatt tactgatact ctttgatttt
    13081 aagactaaag gtacttatga ttggtactta taattcacaa ctagtcttgt tacatctgat
    13141 aaccagaatt acagtacttt tcttttttac ctttttgttc tctaacatat aacaattcgc
    13201 ttaacttatc aatgtaataa ccatcatcac ttatgcgaat taaactagtc agataatatc
    13261 catctttgct tctaggaatc tttttattta ttgctaaatc aatagctgtc tttgctgctt
    13321 caaaatgctt taaatgtgta tgaccttctt caaaactctt tctagtatta taaactacat
    13381 aacctttttt aacttgaaaa actataaatt ctttcttttc atatatttta ttaaacattc
    13441 aatcagccct tctcaagata gttttatagc ataacattag aagcttctaa aaaattttac
    13501 aaagaacata aatattttat aaattataaa tctaaaacta taatttataa agataacttt
    13561 ctgattattt atatgataat tcatatagat taaaagtaaa ttatctttag gttaaacaaa
    13621 agtaaaataa aagactagtt ataatattta tatgaaaaag tacaaaatat agtatatgaa
    13681 tatagaaaat ttaaatcatt tatttacagt ttttatttcc ataagtatat ttaccttcag
    13741 ataccttgtc atcatggaaa tatattcttt cacaactttt atctccccat agaatgtttt
    13801 ttgttatttt atgggacgta tctcctgtaa atacttcagc tacagtagta tctgtaacat
    13861 cacagtaaag agaagcaaat tttttaaacc aaggattttc ctcaaatctt aaaagccaag
    13921 aagctccata ggggcaatgt aactcttcaa attcaggaac ccatcctaga aatggaacat
    13981 cagttgtgct aataatattg tctgcgatta attcatctcc attttcatga gctctctctc
    14041 tcatagcaat agctctttct tcagaccttt caaataaaac ttttcttaat atttcgctag
    14101 ttttatcttc accaaactct tcaaataaca ctttggaaaa ataaaaatat aaatcaccaa
    14161 aatgtttgca tgccatacga acttcttcaa tagcataatc tttgtcaata gtttcattag
    14221 ttacaacatc ttcgttcaaa aaaatcactc cttagaataa gtattagtta caatataatt
    14281 atagaatatt ttttataaat taggaaatta atttgagata gatatatttt taataaatga
    14341 aagtataata ctaaaataac ttattgttac aaaaatcttc taaattaatg tatttaggag
    14401 tttccatatg aatgagagta gttctgtaaa tatcttcaat acatccattt gcaatattat
    14461 taaaaatatc tgtgtgctct tttaatgttg tttcaagcct tccttcatgt gacaatgaat
    14521 cgagagctaa tttttttatt ctgtacatat aattgttaaa cattttgtca aattcagtat
    14581 tttgagcata tgaaactata gttgtgtgaa ataatgtatc gtattctgca aatgagttta
    14641 aatttttatc acctaataaa atatcttttt gtttatctaa aagatgtttt aatttactta
    14701 tagtttctac tgctctagct tctttgtatt gacttgtaat taaaaaagta caaaatcctt
    14761 ctattgcaga gcgtatttgg aatatctcta caatttcatt tgctgtaatt tgatggattc
    14821 taaatccttt gcttgggatt atatctataa atccatcttg actaagtctt tgaagagcat
    14881 ctcttatagg tgttctagag atacctattt cactagcaag ttttgtttca gaatatattt
    14941 cattttctat catttctcca gatagtatca attcttttaa gtagtcatat gcttgttctt
    15001 gtaatctttt aaatgtagcc atacttaata atacccccct aaacaatata aaaatacaaa
    15061 aacttatata attttaagca tacaacatag tgaaaataag gtcaatcttt tgcaatttaa
    15121 attgcagata agattaaaaa atataaaaaa ttattgaagt gtgaagcatg attaacatta
    15181 aaggcagatt aattaaaact aattgaaatt agttaaaata caaaactgta aaagtagtat
    15241 attatatttg aaaacaatag tatgtattaa aacttatata ggatgactaa atataataaa
    15301 tgactttaaa aattactatt tattgttaaa ataaatactt ttatatttta tattgaccgg
    15361 tatactggta tgtagtatac tctaaaacat aaaaaacgtt ttcaaaaatg tgaagatatg
    15421 gagggttata aaatgaaatt gggatttatt ggattaggga taatgggaaa gccaatggca
    15481 aaaaatttat taaaagatgg atgtaatctt ttggtgtatg acattaataa atctgctgtg
    15541 gatgaactta tatcatgtgg agcaaaatac gcaagtgttt tagaaatggg tcaagaatgt
    15601 gatattgtat ttacaatcct tccaaatgga actattgtgc aggatatatt gtttgggatg
    15661 gatggattgg caaaaacttt aaaagaagga agtatagtag tcgatatgag ttcagttact
    15721 cctacagaat caatattgtg tgcaaataaa ttaaaagata tgggcttaga atttatagac
    15781 tcaccagtaa gtggagggga gccaaaagcg attgatggta cacttgcttt tatggctggt
    15841 ggtaaggaag aaatttataa gaaagtagag ccatttttta atataatggg aagctcatct
    15901 attttaattg gagataatgg tagtggaagt gttacaaaac ttacaaacca agtaatagtt
    15961 aatttgacga tagcagcagt ttctgaggca tttgtacttg cagcaaaagc aggtgcagac
    16021 ccagaaaaag tatataaggc aataagagga ggcctagctg gtagtacaat attggatgtg
    16081 aagataccaa tgattatgaa tcgtgatttt aaaccaggtg gaaaaatatc tattaatttg
    16141 aaagatatta agaatgtaat gcaaacagca cacaatttag atgtaccact acctatgaca
    16201 agtcaattat tagaaattat gcagacatta aaagtacatg gacatttgga agatgaccat
    16261 agtggaatag cacagtattt tgaaaagttg gcaggtgttg aaataaaaaa acacgataat
    16321 taatatttaa atatatcaat ataattaaat acatattatt aggattagat tatataaata
    16381 cagcgaggtt aagttatcat agtaaaagaa aaataaaatt gataaataat aaagtagaat
    16441 gataaagggg taaagattat gattacagga tatggacttt tgatagcatt tgttatatct
    16501 ataggaattt tacttgtatc gattattaaa tttaaagtaa atccgttttt ggcattactg
    16561 ataacatcta taattacagg ctttatggtt agaatgccta taaatgaaat atcaaccaca
    16621 atatcgactg gatttggaaa tacattaggt agtataggta ttgtaattgg acttggaata
    16681 atatttggaa atattctttc agaatcaaga gcaacagaat ctattgcaaa ggggttgtta
    16741 gctaagactg gagaaaaaaa cagtgcttta gcagtaacta cagcaggttt tttaatttca
    16801 attccagttt ttatggatgc agcttttgtt ataatgatgc ctataattaa gtatgtttcg
    16861 agagtaacta agaaatctct aatggttttt gtatgtgcct taggagttgg gactatagta
    16921 ggtcatgcac ttgtaatacc tacaccagga cctttggcag ttgcagcaaa tgtaaatgca
    16981 aatgtaggta gttttatact ttattctata attgttgctt ttccagcagc tcttataggt
    17041 ggatggattt atggaaaacg ttttgaaaaa tatccagctt atgctattga tgagaatgat
    17101 agagaaaaaa atttagagca gggaaaagat agcataaaaa ttgaagatga tgattcttct
    17161 aaagtaccag gttttggaat ttctatgttt tctttattgt tcccaatact tcttatactt
    17221 atatcaaatg tatttagtat gttcttagaa aaaggttcta caatgagtgg agtacttgca
    17281 tttataggag ataaaaacat agcaatactt cttggaatat tggtggcaat aggattttta
    17341 aagaaataca taaataaacc tatgggagat gtagttattg aagctgctga ttcagcaggg
    17401 ctaatattat tgataacagg gtcaggtggt gcgtttggca gtgttataaa tgcaagtggt
    17461 ataggtaact tcttagttga tactatgtct ggtcttagta tatcagttgt agtacttgga
    17521 ttcttattaa gtgccttgct tagaatttct caaggttctg ctactgtagc cttagtaaca
    17581 acatcatcaa ttttaggacc tacaattttg gcaactggaa tgtcaccagt tttagtaggg
    17641 cttgctatat gtgctggagg agttggtttc tcccttccaa atgattctgg attctgggta
    17701 ctttcaagat ttagtggatt atcagttaaa gatactttaa actcttggac aattggagga
    17761 accattgctg gagttgtagc atttataatg gttttattgc ttagtgttat aaatggaatt
    17821 ataccacttc caggtctata aataacaaac tattgtttat atcatataaa ttatatttag
    17881 actatacaaa ttacataaag gggcgtttta aatgagagaa gtaaaaccaa ttagcataga
    17941 tatacttaat acttttaaac aagtagatga ggatagatta aataaacttc ttgcagatga
    18001 gcttaagcat ttagatagaa aaattgttgt attagatgat gacccaacag gtgtacaaac
    18061 agttcatgat atttctgttt atactgattg ggataaggat agtatggaac aaggctttaa
    18121 tgagaaaaat agtatgtttt ttatacttac caattcaagg ggatttactg ttgctcaaac
    18181 tacaaaagca cataaagaaa tttcaaagaa tattgtggat gtatcaaaga aagttaataa
    18241 ggattttata atcataagtc gtagtgattc tactatgaga ggacattatc ctcttgaaac
    18301 taatttatta aaaagcgaag tagagagatt atcggggaag ttatttcagg gtgaaataat
    18361 aatgccattt tttaaagaag gtggtaggtt tactatagat aatgtacatt atgttaaaga
    18421 aggagaaata cttgtaccag caggaatgac tgaatttgca aaagataaat cttttggata
    18481 taaatcatct gatataggtg agtggtgtga agaaaagaca aatggaaagt acaaatctag
    18541 tgatatgata tatatttcgt caaaagaact tagagagcta aatattgaag ctataacaga
    18601 aaaacttaaa aaagctgaag gattcaataa aattattgta aatgcaaccg attatgtaga
    18661 tgtaaaggta tttacaattg cgtttatacg agcagtaaat ggtggtaagg aatttatgtt
    18721 tagaagcgct gctgccataa caaaagtact tggcggagtg agtgacaagg aactacttac
    18781 taaggatgaa ttagtatcaa aaggtaatac caatggagga attatacttg ttggttcaca
    18841 tgtgaataag actacccaac agttagaaga acttaaaaat tgtaaatatc cgatagaatt
    18901 tatagaattt aatcaacatt tagttcttca agaaaatggt ctaaaaaatg aggtaaaaag
    18961 agttataaaa gttgtagagg aaaaaataag cagtggtaaa actgttgctg tttatactag
    19021 acgagagcgt tttgacttgg atacgaatga taaagataaa cagcttatga tttctgtaga
    19081 aatatcagat gcagttacca gtataattgg tatgcttaat gtaagaccaa attttattat
    19141 agctaagggt ggaattactt ctagtgatgt tggtactaaa gcactaagag taaaaaaagc
    19201 tacagtaatg ggacagataa aacctggtat accagtatgg atgacaggca aagaaagcaa
    19261 atttcctaat atgccatata taattttccc tggaaatgtg ggagagatta gcacacttag
    19321 agaatcagtt gagatattga tggggtagta tattagtgta ttgaagttta aacgcttaaa
    19381 tttactgagt taaaattaaa ataaactaaa attataaatt aagattaaaa ataatagtat
    19441 attaattagg gtttagagta ttttacaaaa aatattctaa accttaatct tatgtattag
    19501 tatttttatt atttaactta tgtgtttatt ttattattta tcttttttaa ttgatttaat
    19561 attttctttg attttattta aaatatctgt atgcttatta gagcttatgt tggggaatgc
    19621 tttgattata cgtctatgaa gtaatttgtg gttagacagc aaagtattaa gtttattttt
    19681 tagctcatca atacgatttt tagcatagcg ataattttct ttttctttaa gtatttcaaa
    19741 cctatcttcc aaagtatcca gtctattttc aactaaagat tcaagtttgt tttctatagt
    19801 ttcttgtgta ataagtttgt agtattcttt cttatctttt aattccttaa ttataagatt
    19861 gatttgtttt aacttaccat ttaattgtaa aattgtatgg actgtgataa ctaaatctaa
    19921 aacaaaataa atttctatag atatagcaaa tataaatagc acatttttag agatagagtt
    19981 aataaagtca accacaatag gatgtactat aagcattaca atcaatgata atacaccaaa
    20041 cataagtgag ttttccagac aaactcttcc atgaatatta aatttatgtt tagaataatc
    20101 ccaccaagtc atattaaata ttttttctaa aataaagctc gtacagtact ccattatgct
    20161 agttataatt atactaaaca aaaataacaa aggaatattg tgtacaaaag gagtcaaggt
    20221 agcaactatt gctaatgctc caaatccata tatagggcaa aatggaccat ttaaaaaacc
    20281 tctatttaca aattgttttt gtaaaactga acaatatgta gtttcacaac accatcctat
    20341 aaagctataa attacaaaat aaagaaattg aatataaaat gtactcataa gcacctcatt
    20401 tctagttagt ataacactat atactcgata ttttattttt atgattaatt tactaaattc
    20461 aatttacata ttgattatac caatttatag aaatttatca aatttaatta cagttagaac
    20521 attataaaca tggttataat aatgaaattt tatttaaaat aaataagggt gtcaagtata
    20581 atcaaactaa tagttgtaga tactctacac tatgttagtt taattacatt tgacaccctt
    20641 atatttatac tatgactatt tacattcgaa actagagcaa catgtttcaa tataaaggtt
    20701 tactctatca gtttcaggag caaccaatat attatcaagt gtacataagc taccatcttt
    20761 gttgtattta caatttttta cagtacaaac taattcctca caatttttat tattaacgtt
    20821 gctagataaa ttactataaa tagattcatc taaaaatgaa ctacaacaag tatgtctgtc
    20881 attgtgagct ttttcaccat ttataaaaat tttatctgca taacatatat ggctgttgtt
    20941 atgtgaacag ttaattacat tacagtttat tctttccaaa gttattacct cctaaacgat
    21001 tatttattat aaattatgtc catataatta caagaatata accattaaga gaaaactacc
    21061 ttttagaaaa tatctattct tagagtaatt tgaaatataa atatattttc ttctaaaatt
    21121 tacctaaatt gtataaaaac ttacagcttg gagaaaataa tagaaaaagg aggcgtaatc
    21181 aatgtttgaa aagaagaaaa aagaattatt tccttggaaa atatctgcct taatactggt
    21241 tggagtattt gtattaagta gcggagttta tatagggaca agattaaaac acaatggagt
    21301 tcaaactggt gaagttgtaa gtactagtgc gaatatgaat gaaaagtcaa aagaagcttt
    21361 tggattaaat aaaaattgtg aagtttggct tcaaagtgaa ggcgaaaatg aaactaacta
    21421 taagagaaca cttacaatgg caggaatagt tccagagtca ttattagata agacgaagtc
    21481 agaaatagta tcatatttta aaaaagaata tccaactaaa aaaataaaga gtatggataa
    21541 aaatgaaatc atattggtag aatctaaata tagtgataat aaagatgtac aagcaatgga
    21601 tgttactata aaaggtaaat atactattga aaatgataat ggaaatatag cactatataa
    21661 atatgatgat agcggaagta aaacattagt agaaaaaaca aaaataagag tagattcact
    21721 tcctaaaaca gttcaagatg aaatcaaaaa aggtgtagtt atggatactg aagaagaggc
    21781 ctattcaaga ttagaagact ttgcaagtta atagtaaatt aatattattt taagttaaat
    21841 actaagtaaa ttaataataa ataatttaat attaaataga ttagtattaa aattgtagtc
    21901 acatttaatt cctataaaaa tacaataatt tatttgtaat aaatataaaa gttatattga
    21961 ggtaaaatct tgctttgata taactttttt atttgatttt atattttaca tagagcattt
    22021 tatgatttgt taatattatt tatgatataa tgatttagaa cataagtttt tatgagaaat
    22081 acattttggt atttctttta tttttagggg gaattttaaa aaatgataat attggggatt
    22141 gacccaggta tagccatagt tggatatggt ataattgaat acaaaaatag caagtttaaa
    22201 gcaatcgatt atggagcagt tacaacacct gcccatatga atatatcgag aagattggaa
    22261 cttgtgtata aaggaattga tacaatagta aagaattaca atatagatga agttggaatg
    22321 gaagaattat tctttaacaa gaatgtaaaa acagctataa cagttgcaca agctagaggt
    22381 gttactatgc ttgcatgtgc tcataatggg aagcctgtat atgaatacac tccacttcaa
    22441 gtaaaacaag gtgtagttgg atatgggaga gcagataagg cacaagttca acagatggta
    22501 acttcatttt taagtctaaa aaaagttcca aaaccagatg atgttgcaga tgctttagct
    22561 gtggctattt gccatgctca ttcaaacaaa cttgaaaaaa ctctaaagaa tataggtggt
    22621 aagtatgtat agttatataa aaggtacagt agaagaaatt tatatagaca gtatagttgt
    22681 tgaaaataat gggataggat ataaaataaa tgtatcatcc aatacaataa tgaatttaca
    22741 agtgggagaa gccacaaaaa tatatactaa attaattgta agagaagatg atatgagtct
    22801 ttgtggtttt gtaagtaggg aagaacttaa gatgtttgaa ttactaacat ctgtatctaa
    22861 aataggacct aaggtggcgc tttcaatact atcatttgca tcaccagcac agttaggagc
    22921 atatatttta agtgaagaca ttggaaaatt atccaaagct cctggtgttg gtaaaaagac
    22981 agcagaaaga atcgtcttag agcttaagga taaggtagac aaaaataaca tagagtttga
    23041 accaacgtta ttatcacaaa aacctacctt gatatcacaa gatgagtctg tagatgcact
    23101 tgtagcttta ggctatactt tgtcggaatc aaaagaggca gttcaaaaat gtaaaaaaga
    23161 tggtatgaat acagaagcta ttataaagaa agcacttaca tatataatga gtaaatcctt
    23221 gaaatagtat ttggatttta taatatttgg attttaaaac aatatttaag gagtttatgt
    23281 aaatttgaaa atatataaag tagattttca aaagtacgag aggaggggta taaatgcaag
    23341 gttttgaaga tgaaaatagg ataataactt ctacaatgaa gatggaagat atagatatag
    23401 aaaacagttt aagaccaaaa actttagaag attatttggg tcaagaaaaa tccaaagaac
    23461 aacttagtat atttatagaa gcagctaaat cgagaaatga gcagttagac catgtgcttt
    23521 tatatggacc accaggtctt ggaaaaacta cactagcaag tataatagcc aatgagatgg
    23581 gggtaaattt aagaattacg tcaggacctg caatagaaag agctggagat ttggcagcca
    23641 tacttacgaa tttaaatgaa aatgatgttt tatttataga tgagattcat agaatcaata
    23701 gaagtgttga agaagtttta tatccagcaa tggaggattt ttgcctagat ataataatag
    23761 ggaaagggcc atctgctaga agcataagac ttgaccttcc taaatttaca cttattggag
    23821 ctactacaag agctggtatg cttacaaatc cacttagaga tagatttggt gttatttgta
    23881 aattggacta ttatacagtt gatgaacttt caaaaatagt acttaggtct tcaagcatct
    23941 tagatgcaga gatacagtct aatggagcat tagaacttgc aaaacgttca agagggactc
    24001 caaggatagc aaatagatta ctaaaaagag ttagagattt tgcacaagtc agagctgatg
    24061 gaaaaataac tgataaagtg gcaaaagatg ctctggaact tttaggagtc gatagtttgg
    24121 gtctcgattt tgtagatgag aagcttctta tgactataat agaaaaattt agaggaggac
    24181 cagtgggttt agatacattg gcagcttcta tcggagaaga tagaaataca atagaagatg
    24241 tatatgagcc atatttgctt caattaggat ttataaatag aggtcctaga ggaagagtag
    24301 ctatgccatt agcatatgaa cacttaaaaa ttccatatcc caatgaaaaa taaaaataga
    24361 ttatacgagt atttataaaa taaaagagtc attaaattaa aatatttcta aaatggctaa
    24421 aaattcaaac atatccacaa aatggtaatt atatctgtag atactgtgga taaacacgat
    24481 aaaaattgtg tgtattgtat ctatatattg tggataaggc atgtatatcg tggaaaaata
    24541 tctatatatt gtggatatgg tgtctgttaa aaatagcaat ttcttgatag attatggtaa
    24601 tattgtggaa aataagttgt ttttttgata ttattgtagc tggagaaatt ttaagagagg
    24661 aagtttaaca ttgaaaacaa gtgattttaa atttgactta ccacaggaac ttatagctca
    24721 agttcctata gaagatagag ctagctcaag attgatggta ttagataaag agacaggtaa
    24781 tattgaacat aaagtattta gagatataat agaatattta aatccaggag attgcttagt
    24841 attaaataat actagagtta taccagctag attaatagga gagaagcttg aaactggagg
    24901 taaaatagag tttttattat taaaaagaac agaggaagat acttggcagg ctttagtaaa
    24961 accaggaaaa agagcaaaag tagggactaa attttctttt ggaaatggaa agctaattgg
    25021 agaagttgta gatttatcag atgaaggttc aagaattata aaattccatt atgatggaat
    25081 ttttgaagaa atattggatg aacttggaaa tatgccacta cctccatata tcactgctag
    25141 actagatgaa aaagaaagat accagactgt ttattcaaag cataatggtt cagcagcagc
    25201 acctacagca ggattacatt ttacagaaga attgttaaat aaaataaaag aaaaaggtgt
    25261 agatatagcc tttgtaactc tacatgttgg cttaggaact tttagaccag taaaagtaga
    25321 ggatgtttta aatcacaaga tgcattcaga atattatatg gttagccaag aagcagctga
    25381 caaaataaat agagcaaagg aaaatggaaa aaatgtaata tgtgttggta ctactagttg
    25441 tagaactata gaatcagctt gtaatgagga tggaaagatg aaagaaacta gtggatggac
    25501 agaaatattt atatatccag gttataaatt taaagtttta gataaactta ttactaattt
    25561 tcacttacca gagtctacac taataatgtt agtaagtgct atttgtggaa aggataatgt
    25621 tttaaatgca tataatgagg cagtaaaaga aagatataga ttcttcagtt ttggagatgc
    25681 tatgattata aagtagggag gtaatagatg catagtttac aaaaacaaaa aaagaataac
    25741 cctatattaa agattgcttt tatatttgaa cgtgttttag cagtagtagt tctaatagca
    25801 gtatttttag gaactataga tgttctaaga cttatgtggg gagcatatat agttgatttt
    25861 caaaatccag tacaatacag tcagcttaat gattttttag cacaaatatt acttttagta
    25921 ataggtgttg aacttgtagt aatgctatct cttcatatac caggagcatt tattgaggca
    25981 ttactttacg caattgctag aaaaatgttg ctactgccta aaaatgaagg tatgatagat
    26041 gttttaatag gagttatagc tatagcagga ctatttgcta taaaaaagtt tttagtaaca
    26101 aaagacaaga gtgctcttaa tataatgagt attcatgatg aagaagcaat taaggaacaa
    26161 cagaacaaag aagacgaaga taaatctatt tagacaataa attatatgaa tagcttatta
    26221 caaatatatc ttaagaaata atgagaaata aatatgatta aggagaactt tatgtacgca
    26281 gtaagatacg aattgataaa gacttgtaag cagagtggtg caaggttagg aagattacac
    26341 actccacatg ggattattga aacaccaata ttcatgccag ttggaactca agcaactgta
    26401 aaatctatga caccagaaga attaaaagag attggttcac aaataatact tagtaacaca
    26461 taccatttat atatgagacc aggtcatgaa cttataaaaa gagctggtgg tcttcataag
    26521 tttatgaatt gggataaacc aatacttaca gatagtggag ggttccaggt atttagtcta
    26581 gggcctttaa gaaagataaa agaagaaggt gtagaattta ggtcacactt agatggttca
    26641 aaacattttc taactccaga aaaagctatg gaaatacaaa atgcattggg ttcagacata
    26701 atgatggcat ttgatgagtg tgcaccatac ccatcagata gagaatatgt aaaaaattct
    26761 ttagaaagaa caacaagatg gttaaaaaga tgtaaagatg cccataataa taccgacaag
    26821 caagctttat ttggtataat acaaggtggg atgtataaag acttaaggga acaatcagcg
    26881 aaagaaataa ctaatattga tttaccagga tatgctattg gtggacttag tgttggagaa
    26941 ccaaaaccac ttatgtatga cgtgttagag catacaactc cacttatgcc aaaggataaa
    27001 ccaagatacc taatgggagt tggtagtcca gatgacttag tagaaggtgt tataagagga
    27061 gtagacatgt ttgattgtgt cctacctact cgtatcgcta gaaatggaac tgcaatgact
    27121 agccaaggta aagtagtagt tagaaatgca acttatgctg aagactttac tccacttgac
    27181 cctgaatgtg attgttatgc ttgtaaaaac tattcaagag cttatataag acaccttata
    27241 aaagcaaatg aaatactggg tgcaagatta ataactactc ataatcttca ctttttatta
    27301 aatttaatga aacaaataag acaagcgata atggaagata gattacttga ttttagaaat
    27361 gaattttttg caaaatatgg atatgaaata taggttttgc actattaatt ataatcattt
    27421 taatatataa tattcaatag tactcaaaga tagagaaagt aaattgttat ataaacattt
    27481 attatgattt acaaaagagt ataatttaca aaggagtgaa aaattaatgc cacagcaaca
    27541 aataattatg agtataggtc tatgggtagt agtaatcgca atcttttatt tcctaatgat
    27601 tagacctcaa aagaaaaaag acaaacaatt aaaagaaatg agaagtagcc taagtgtagg
    27661 agacaaggtc attacaatag gtggaatagt agctaatgta gctaaagttg aagatgatgt
    27721 tgtaatatta gaattaggac caaatagaac taaagttcca tttgaaaaat gggctatagg
    27781 aactgttaaa tctaaaaaag aagaaataga agaagactaa attagtaatt taaaagcctt
    27841 ttctcgaata tatattaatt tgagaggagg tttttttatg ggaaaatcta attatatttt
    27901 taaaggtcta ggatatgcat atataataac tttggctgtt ttattagtgt ataacttatt
    27961 tttgacattt acagatattg gtggagataa tataactatg gtctcatcat ttataactac
    28021 tatttctgca gctataggag ggttttacac ttcaaaacac atgaaagaaa aaggacttat
    28081 gtatgggctt ttagtaggac ttttgtacat agtatgtatt tttttgactg tgtttttagc
    28141 acaagaaaag tttgtatttg aagtagggat gatttataag ttattgttaa tatcagcagc
    28201 aggaggcatt ggaggcgtat tgggtgtcaa ctttaaatag agtttatatt aaagtagttt
    28261 tatgatataa tcaataggaa acaattagaa atggaggcgt tgaaaatgga aaataaacat
    28321 gtaaaaactt tatctcaagc tactttaaag caaagtgctg caaaaggtgg atgtggagag
    28381 tgtcaaactt cttgtcaatc agcttgtaaa acttcttgta cagttgcaaa tcaagagtgt
    28441 gaaagataga aaattattga tttagttgtt tgtaagaggg tatctcaaag tgattctttt
    28501 atcattatga ggtatcctct tttattttca attattatat aaaaatatat attctattta
    28561 tgtaaaatta aatttgattt cttatcgttt aattatgcaa aaatatctaa tcatataaga
    28621 gcatttattt taaagtagct actcttatat tttatttttt atcaattaca tgtattagaa
    28681 taatttttaa ttttaaactc taatttatga tgatttttat aatatgttat atattttaat
    28741 atatggagat agatattaat ttagtgatat atatttaagt aataagagta aagcaaaaat
    28801 tagtgcatag tagtatataa aaatggagtt tgaatacttt tgttagtata tgattgttta
    28861 gtagaaaatc actataaaat aggatttcta tctttgagca taattataga gagctaagca
    28921 gggggaaggg gttcaagaag atattattag tgtttgtgtt agtaaattat gtaaacacat
    28981 aaaatatatt gaaaaataat attatatata atattattta tgtgtgggaa atacgaatat
    29041 aaaaaataaa aaataatatt cggaggacat aaatatgtta agcaacaaaa agagaagtat
    29101 ggcaatagta atggcaggtg ctacagttat gagtgcagca gcaccaatat tcgcagacaa
    29161 tactgtgaca gaaaatgttg ataaaaatta cacagtaagt gcgaaagatt ctgctaaact
    29221 aatcgaagag gttagaaaag cattagaagt taagtttgaa gatacaaaag caggagcaaa
    29281 tgtaaatgat agagtttacg atataaaagt agacaatgta aacttaacta atgcaactca
    29341 attacaaaat aagataaact ctttaacaga agggcaaagt ttaaaagtta ctattcaaga
    29401 taaaggacat caagtgttag gtggaaaagt agttgactat aaaattgaaa actacaaaac
    29461 tgctcaagaa atagtagatg cagttaatgc ttacaatgca actttagcag aagattctga
    29521 taacaagtta acagcaacta taaaatctac aaatacagtt gaagtaaaaa gagcaaaaga
    29581 ttcagcaaat gttattacat taaatgtagg agaccaacat ttagatttct caaaagtgat
    29641 aacatcagaa gaaggtactt ttgaaggtta tgaaaagagt tatagtgaca tagattcaaa
    29701 agaattacat acgataacag ttaaaaacgc agatttacaa gacatatcag cagaagaatt
    29761 atttgatgga attagattaa ctacattagg aagagaaata gttaataaag ttaaaaatgg
    29821 atatgcttta acatttgaaa atgaagcaat ccttactcaa gaacaagaag atagtgatga
    29881 taaagataaa ccagaaaaat caagctttga tatagtttta agcaaggcaa atgaaaagcc
    29941 agaaacaata agtgtttcaa gtaaaaacca taagttagtt agagacttac acaaagtatt
    30001 aactgatgta aaaggtggaa aagaattaaa agtagaagtt ttatctggtg attcaagatt
    30061 tacaacagca gtagaagtta gtaaagagag attcaaagat ggagaagcag aagctataat
    30121 tttagttgga gaagatgcta tagttgatgg attagcatca gcaccacttg cctctcaaaa
    30181 aaatgcacca atattattat ctaaaaaaga ttcactacca tcagaaatag aagctgaaat
    30241 attaagagta cttggaagta atctatcttc taagaaaata tatatagtag gtggagaatc
    30301 taaagtatca aaagaaactg aagaaaaact ttctaaatta ggtgtaagta aagttgagag
    30361 agtttctgga gaagatagat ttgaaacttc tttagaaata gcaaaacaat taaaagatac
    30421 atttaagact gcttttgtag taggtggaaa tggagaagct gatgctatga gtatatcagc
    30481 tagagctgct caatttggtg ctccaataat agttacaggt aacgaattag atgcaaatgc
    30541 tgaaaaatta ttaaaaggaa aagaattaga aatagtgggt ggagaaaatt ctgtatcaaa
    30601 agaagttgaa gacaaattag ttgatataga tttaaataat aaagttgaaa gattagctgg
    30661 agaaaataga aaagatacta atgctaaagt aatcaataaa tactatgcag gtgcaactaa
    30721 agcatatgta gcaaaagatg gttatgtagg tggaaatgga caattagttg atgcacttac
    30781 agcagcacca cttgcagcta gttcaaaggc tccaatagta ttaactacag aagaactttc
    30841 taaatcacaa gaagaagtag ttgagttaag acttaagaat gctactaagt tagtacaaat
    30901 aggtgaagga atagctaaaa acgctattga aaaaatagca gaaaaaataa acttatttac
    30961 taagaactaa taagatatag gaattaatat taaaagtttg tttagataat atatgattat
    31021 acaatgcgtt aatatgtaag acatagtgta gatattataa aatatacaaa taacaagata
    31081 aaataataaa aataaggtgt ctcatttaga
//
LOCUS       DAYATN010000035        29660 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.035, whole genome shotgun sequence.
ACCESSION   DAYATN010000035 DAYATN010000000
VERSION     DAYATN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.035"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(249..1118)
                     /locus_tag="ACGI8A_001545"
     CDS             complement(249..1118)
                     /locus_tag="ACGI8A_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0179546.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(1215..2072)
                     /locus_tag="ACGI8A_001546"
     CDS             complement(1215..2072)
                     /locus_tag="ACGI8A_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0179547.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(2287..4305)
                     /locus_tag="ACGI8A_001547"
     CDS             complement(2287..4305)
                     /locus_tag="ACGI8A_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFH0179548.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIINTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(4576..5352)
                     /locus_tag="ACGI8A_001548"
     CDS             complement(4576..5352)
                     /locus_tag="ACGI8A_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFH0179549.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLIDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(5409..6380)
                     /gene="asrC"
                     /locus_tag="ACGI8A_001549"
     CDS             complement(5409..6380)
                     /gene="asrC"
                     /locus_tag="ACGI8A_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HFH0179550.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(6393..7184)
                     /gene="asrB"
                     /locus_tag="ACGI8A_001550"
     CDS             complement(6393..7184)
                     /gene="asrB"
                     /locus_tag="ACGI8A_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HFH0179551.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(7185..8192)
                     /gene="asrA"
                     /locus_tag="ACGI8A_001551"
     CDS             complement(7185..8192)
                     /gene="asrA"
                     /locus_tag="ACGI8A_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HFH0179552.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            complement(8583..9272)
                     /locus_tag="ACGI8A_001552"
     CDS             complement(8583..9272)
                     /locus_tag="ACGI8A_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFH0179553.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(9335..10183)
                     /locus_tag="ACGI8A_001553"
     CDS             complement(9335..10183)
                     /locus_tag="ACGI8A_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0179554.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(10202..11089)
                     /locus_tag="ACGI8A_001554"
     CDS             complement(10202..11089)
                     /locus_tag="ACGI8A_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFH0179555.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(11147..11950)
                     /locus_tag="ACGI8A_001555"
     CDS             complement(11147..11950)
                     /locus_tag="ACGI8A_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFH0179556.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(12110..12562)
                     /locus_tag="ACGI8A_001556"
     CDS             complement(12110..12562)
                     /locus_tag="ACGI8A_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFH0179557.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(12692..14194)
                     /locus_tag="ACGI8A_001557"
     CDS             complement(12692..14194)
                     /locus_tag="ACGI8A_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HFH0179558.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(14227..15108)
                     /locus_tag="ACGI8A_001558"
     CDS             complement(14227..15108)
                     /locus_tag="ACGI8A_001558"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFH0179559.1"
                     /translation="MRTTDMKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGL
                     YVGGSTGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGY
                     DAISAVTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENE
                     KIIGVKFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRA
                     RQIFELTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKE
                     ATDEMKSRAKEIYRKYF"
     gene            complement(15178..15849)
                     /locus_tag="ACGI8A_001559"
     CDS             complement(15178..15849)
                     /locus_tag="ACGI8A_001559"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFH0179560.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(16122..16613)
                     /locus_tag="ACGI8A_001560"
     CDS             complement(16122..16613)
                     /locus_tag="ACGI8A_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFH0179561.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(16635..17999)
                     /locus_tag="ACGI8A_001561"
     CDS             complement(16635..17999)
                     /locus_tag="ACGI8A_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFH0179562.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            18418..19593
                     /locus_tag="ACGI8A_001562"
     CDS             18418..19593
                     /locus_tag="ACGI8A_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFH0179563.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(19766..21163)
                     /gene="asnS"
                     /locus_tag="ACGI8A_001563"
     CDS             complement(19766..21163)
                     /gene="asnS"
                     /locus_tag="ACGI8A_001563"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFH0179564.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(21240..21473)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(21642..21836)
                     /locus_tag="ACGI8A_001564"
     CDS             complement(21642..21836)
                     /locus_tag="ACGI8A_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HFH0179565.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(22109..23782)
                     /gene="cspC"
                     /locus_tag="ACGI8A_001565"
     CDS             complement(22109..23782)
                     /gene="cspC"
                     /locus_tag="ACGI8A_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid germinant receptor pseudoprotease
                     CspC"
                     /protein_id="HFH0179566.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLEPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(23792..27190)
                     /gene="cspBA"
                     /locus_tag="ACGI8A_001566"
     CDS             complement(23792..27190)
                     /gene="cspBA"
                     /locus_tag="ACGI8A_001566"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional germination protease/germinant
                     receptor pseudoprotease CspBA"
                     /protein_id="HFH0179567.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(27357..28553)
                     /locus_tag="ACGI8A_001567"
     CDS             complement(27357..28553)
                     /locus_tag="ACGI8A_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFH0179568.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSISYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(28574..29395)
                     /locus_tag="ACGI8A_001568"
     CDS             complement(28574..29395)
                     /locus_tag="ACGI8A_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0179569.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         9589 a         5298 c         3002 g        11771 t
ORIGIN      
        1 aaagttactt aataaaaagt tactttactt tttattattt aaattcctgt ttttattata
       61 ataggtaaaa ttctattaaa ctagttcaca ttttcattat tattgatata ttatatatga
      121 atataccatt ttgtttttta ataaaaaatc atgctacatc aatagtaagc aactattaat
      181 gtagcatgat tttttgtatc taaataatct tagtatttac ctatcagtat agattgatat
      241 atcaaaaact atagtcttct atattcttct ctttttagct tttggttact tagatagtaa
      301 tcaatttcat caattagacg ttgtttatca tcaggtaata caccttttaa attagcataa
      361 tttgatgcat gattacttct aaatacaatt ttttcattag catctatatt ctttatcatc
      421 tctttaattt cttgtaacac agccttatcc tctagtattg taaactctcc tgccttaagt
      481 aaatcgtaaa gttcagtatt aggttctacc attaaactaa gtacacccac ataatctggt
      541 gaaattgctc ctagcatttt tcctgtatca actgcatgct gatgtgtttt tttagttcct
      601 ccaagacctg ctattacagt ggctgataat ttaatcccac tttttttaac ttttctacca
      661 acttttatta attgttcact actaaaacct ttcttaatat cttctaatac ttcattgctt
      721 ccactttcaa gccctagata aatcatttta acaccaagtt ttcttaattc tttaagttca
      781 tcatcagatt ttttctctat agatttagga gagccgtaca tagtaattct ttcacactct
      841 ggaaaaactt cttttatata taaaataata tctcttaatt tctccattgg tattatcaat
      901 gcatctccat ctgctaagaa tattctttct acatgcttat aatatcttct aaatatatca
      961 atttcatttt ttattacttc tagtggcttt attttaaatt ttttagattt gtacatacta
     1021 caaaacgtac atttgttatg agaacatcct agtgtgactt gtattataag actataagcc
     1081 tcacttggtg gtctgtaaag tggcatatcg tattccatat taaaatcccc cttgtaataa
     1141 aatattcatt ctattatatt attaatattt actttaaata ataacttata aaattatatt
     1201 ttttaaattt tagtttaaga tatattcttt aaaaatttaa tgatactatt ggcagtaata
     1261 ttaagttgtt tttcttgact tataatagca tcattatctc ccttttgttt tccataatct
     1321 ccaaattgtg catgatttcc tccttcaatc tcaacataag ttgtatcatc aggaagtttt
     1381 tgttttgatt ctacaagact tttaaaattg actactccat ctttgctccc ccatatagat
     1441 ataacttttt ttcctaattc tttcaactca tcacccattg gataagaagc taaaaataca
     1501 acgccttcaa ttaatttatt atcacgtgca aaatttgatg ctgcaactcc tcctaatgaa
     1561 tgacctccta ttacccaatg ttctatgttg ttgtaagaat ccataacttt ctgtgcctta
     1621 ttagttccaa gtatagctag attaagtggc atctttacga ttacaacttc atatccactt
     1681 tctgctattt ttcttgcaag tggagcataa gattctattt ctactttagc tcctggatac
     1741 aaaattaaac ctttagaaac ttttcttcct tttggtgtaa atgaaatgtt ttcatttaca
     1801 gttacttcaa ctttactatt gctgactaga gcttcttttg ccagcttttg aggttcatat
     1861 gttttattta tccatatgaa aaagccaagc aaaatcaata caaatagtat acctattccc
     1921 ttaaatatct tattctttat gttaaatttt ctattattat ttttatcatc tttgttctca
     1981 acctcatcat aaagtaaagg tctctcaagt tccatagtta tactaaatgt atcatatttc
     2041 ttattacgct ttacctttac atctttactc ataaatctaa cccccataat atttgaatac
     2101 tattatagtt tatcctaagt aaattgtttt ttcaaccaat gaaaaaaatc aatattttaa
     2161 ctattttcat ataaaattca taaatatatg ttttttgttt tttatatatt attttctttt
     2221 agtattttat aaaacaaata gccatatact ttattttaaa tatatggcta ttctataatt
     2281 taatatctaa gctgcttctt ttattgaaac tttagttata ttacttattc tatacatttc
     2341 tttgacacca tttagagttt gcagtaagtt tagacatatt atcttgtcac ctagactttt
     2401 agtatttata atactctcta aaattacact tcttccttca tcatacttat ctacattgta
     2461 atttgaaata taattagtat ctctcaatat tttttcaaca tcttcatctt ttattttatt
     2521 tttattaaca tttatatata gattatacat attacttatt agtaatttct gattttctaa
     2581 aaaatcctct tgatgtttat ctttatatat agcatttatc aacataagtc tatcttcaat
     2641 aagtgatggt atcaataata aattttttat atagtaaaca tcagctacat tttctataga
     2701 cttattgact tcatcaatta tatttttaac tattccttta tacatttcta taacatgttg
     2761 atagcctgtt ttagcatcat atggtaatat aaacttattt actattaatg caataattgc
     2821 tcctaatgct acatatgaaa tcctgcttat ggttaaaaca tttgggtctc ccataactac
     2881 tgctgaacca agagctgaaa cagttacaca taccattaat tttctataat caactacata
     2941 agagtttata taccctgcta aaagaactat taacgacctt aatgtactat cttttattat
     3001 tgaaaataat acaataaata ttacagctcc tataaatgta ccttctattc tttttctaga
     3061 cctttgtata caattttctg cgtaaggttg tgttaatgaa aagacagtta acattatcca
     3121 tcttccatct cttaaatgga aataatccat aataaaaccc gctacagctg tagctaaagc
     3181 aatctttact gcataagaaa atcttataga atttaaatta aagtttttct tatatattga
     3241 tagtcgttta aattcatgag gtatctttga ttttctttca gctttctttt cgtctcttga
     3301 ttttactttt ttataatcta ctaaaaactc atataaattt tctaaaacac tataaatttc
     3361 ttttaaattt atatttttag tatcctgagc aataacataa tctctaaatc ctaatatctc
     3421 tttatcagtt atggtatctt tattttctaa acacatctta atatagttta tttctttcgt
     3481 tgctacaact agaacatctg atttactatt tatgctactt tcttctaaaa tattttttat
     3541 atcttcatct tcaaattctt tactatcctt tttgtatctt tctaggatta aacttattct
     3601 ttctaatgaa aataaaatat tcattatgtc tatacctcta atactgatat aaaagtcatc
     3661 ttttctatta tcaaaaataa ccattttcaa atttttagat atagaattta ttctataatt
     3721 acagctatct atttttctca tactagattt aattatagaa ctatcatcat taatagtatt
     3781 ttttaaaaga ctaatttttt ctaataattc atcacatata gatatcagtt ctttatctcc
     3841 gacatttttc atcctatttc tattcataac aaattgagca atcattatta taactgctcc
     3901 aacagctaaa gccgacaatc tcataggaaa ttcacttttt cctacaggta tacttaccat
     3961 aaatatatac attagaccaa agggtacata tactgctctt tttaagtcat aactaaatac
     4021 ataccctatt ataaataatg ctataaaatt taataaaact cctaaataca tattttgtac
     4081 tgcaaaaaat gatagtattc ctaagattat atttattgct aaatacttca gtaaattttt
     4141 aattggagaa atagttaaat ctctttccaa gagcgttagc atagctgtta ctatacctac
     4201 acctataagt gtatttttac ttccaaataa atatataaat atagaaataa atgctattat
     4261 aaaaataaac aactttgttt ttgatattac tagtttcttt gtcatcatat cccccctaaa
     4321 aatttcaaca agaccaattt tatcatattt gttatcttat tcaaaatgcg cttttttacg
     4381 gtaggacttt aacttttttt atatttagaa ctatataatt catatataaa cgtatttttt
     4441 atatatataa ccgatatatt ttcgtatgct cccatatttt ttttgtttca aatatttttt
     4501 caaaaatata aaaataactc tctcaacaat actaaaaatg catttatcaa gagagttata
     4561 atttatacaa taaatttaat taagcttttc tttacctaat atataagttc caagacccat
     4621 aaataaagct cctccaacaa tatttccaag tgttacagct actaaattat aaatagctcc
     4681 tccaatagta actgtattta ttgttggtga aaataatgaa acactataaa ttgtcatatt
     4741 tgctacacta tgctcaaagc ctgatgttat aaaagcaaat aaacataaga atatcataat
     4801 tatttttgca gtatcactat tagttctaaa cgaacacaac actgatacac atactagaat
     4861 attacataaa atccctctaa agaaaagagc tgtaaatggc atggatgctt tactagctgc
     4921 tgtatttgca aaaaattcag ctacaggtcc tttgtctatt agaccagttc ctacaaatat
     4981 aatgcctaaa accaaagctc cgattaaatt acctacccaa ctgtatgccc atattttaga
     5041 agtatctttt atacttacac ctttatttaa cataccagca gacataacca tattgtttcc
     5101 agtaaataac tctgttcccg tcattataac caaacttagt gctattgcaa atgataaccc
     5161 cattactatc ttagtcatag gtgaacctgc atcagtcaaa agtcctccaa tagtaaatat
     5221 caaaagtata cctataccta cataaagtcc tgcaaacgca gaagatacca aatattttac
     5281 tttacttgtg tttaataaat ttatcttatt tatagctgca ttagttaatt tatccaaagt
     5341 ttctttatgc ataattccct cttccttcta ttaaatttta atttattcaa atatatgttt
     5401 tactcaattt atagatattt aattccactc cagtatatgt tttcgtacat tttagttatt
     5461 tctggaagct ctacaccttc aagtgcccat tttttaaatt ccataaatcc tgttctatca
     5521 attatatatc ctatatgttc ttttcctcct ggtgcatctt tatctatata cttttctaca
     5581 tatctataag tattaagtat tatctttatt atgctgtcct catctaccca taaaagaaaa
     5641 tcttctgcta gtctagggtt tttctttcct gttctaccca ttatagctaa tctatagtat
     5701 tttttctcat ctctagtcca tgcatttgtt gggcaattta gcacacactc tccacagcct
     5761 atacacttat ctttatctct taccacctta ttattttctg tttttaatgc tccaacagat
     5821 atcttagaac atttttttac acatgctcca caatttacac atctatcctt ttcatatatt
     5881 ggtaaagcca taccaataat tccaaagtca ttcattctag cttttataca atcatttgga
     5941 caaccagtta aagccacttt aaaatgtaaa tcatttggaa atatagcttt ttctattctt
     6001 ttagcaaatt cagttgtatt atattgagcc tttggacaga ctttatttcc tatacaagct
     6061 gctatatttc ttgtacctgc tgctggataa cctgcgtttt tctcactttg atttatattc
     6121 attttttcaa taacaggttg tataatttta tttatttctt ccatatcttc catatttatt
     6181 cctaatattt caaacccctg tcttgtagtt atgtgcacct gtccattgcc gtactcactt
     6241 gcaattttag aaactacagc taaactctca gggtcaataa gacctccagg tactctaact
     6301 cttaaagaag tctcatattt tgtttttgta attctataag catttttcat tacttttttg
     6361 gtatttaaat ctcttatcac aaacttcacc ccctaatcaa taagattttt agcatatgaa
     6421 taatcaaata ctggtccatc taaacatatg taagtatcat ccattttaca atgtccacat
     6481 ttacctattc cacaacacat ttttctttca taagaaaccc aaatgttctt ttcatctaaa
     6541 tctcttttta aaaattcttc tactgtaaat ttcatcatca ttggaggtcc aactacaact
     6601 atagaagtat tgtttatatc tttcatatcc aattctggta tgtattttgt tactaatcct
     6661 acattaccac tatatccttc ctctgctcca tctacagtaa ctaaaatatc aagtttttta
     6721 ctccatctct ttaaatcatc tgcaaataaa atatcttttg gtgatttaaa tcctactatt
     6781 aatttaaact tttcgcattt ttctttatta ttataaaaat attcaactat tcctcttaca
     6841 ggggctaatg cacttcctcc tgccactact acaatttctc ttccttcata aagggataca
     6901 tcaaatccat ttccataagg acctcttata aaaaaagaat ctccttcttt ataattaaac
     6961 aactcgctag ttacttttcc tacatttctt attgtaaagt caatataatc ctctccatat
     7021 cctgatactg atattggact ttcaccgtat ttaggtatag atatctcata aaactttcca
     7081 ggcaatacac cttttgtatt gcagtttact ctaaaagtcc attctttatc agtatgttta
     7141 actatatcta gtatttttgc acttactggt atatatgggt tcatttatac ttcctcctta
     7201 ctaattacta aatcatttat tttttctata cactttgaaa atgatatgta ttgcggacag
     7261 gcatcatcac atcttccaca gccaacacac atatgatatc cgaatctttt tttaaaatca
     7321 tgtattttat gcatagtttt aaatctcatt ctttcgcctt gtttttttct aaaagaattt
     7381 ccacctgcta tatctgtata tccatctact tgacaagaag cccaaaccct tcttctctca
     7441 cctacatttt cgttttcctt atagaaaata tcctgcattg taaaacatga acatgttgga
     7501 catacaaaat tacatctacc acatcctata catcttgtat catattcatc ccatattgaa
     7561 tttcctatca tttcatttga atcaatattc tctggcacat ctacagatat taaattatca
     7621 gttacataat caacattaaa ttcagttgct tctccattaa atacttcaaa ttcactatcc
     7681 tttatatcta agtacagtgt atctccttct atatttaatc caattgaata attatcagtt
     7741 ttgttagaat ccatgctcac acaaaaacag tttctaaaac tttccttaca ccctacaagt
     7801 gcaaacttta ctttatctct gatattttta taaaaataat ctttttcttc accattattt
     7861 agatatattt catcaagtct ttttatacca tttatgtcac aagctcttaa gaaaattaaa
     7921 attttcttgt tgtctaaatc gctttcctta aactcttttt cagtaaaata aaacaatact
     7981 tgatttatgg gaagtacaac ttcttttggt gaaaagtttg ctttcttagc aaattccatt
     8041 tcttcaaaag cgtttacctc cttgtatctt ataacatcag tatctgaaaa agtaccttta
     8101 aatgggatta aaactggtgc aaatatcttg tagtcttttt ttagataatt cagaccttca
     8161 ttgaacttag atgaatctaa ttttagtttc atttttatca cctttcttat attttagttt
     8221 tacttacttg ctatcttgtt taaatcttaa cactttattt tctatgcttc cgtgataatt
     8281 atcacagttt caaaaataat aaaaaaaata ttaatggagt ttattaagtt ctgttaatat
     8341 ttttttatta tctacattat tcgatttcaa atctaatcac ttaaatacaa tcattaagta
     8401 caataaaact actatctact caattttttg aataaaaaag aatcttattt gtttttttat
     8461 acaattcaat agggtaaaat aaaaattaat aaattagaca ataaaaaaga atctataaat
     8521 agattctctt aataatattt ttatttaatt aaaattctaa taatactctt acttaatcat
     8581 aattaattat ttttaaaata ccttgataat tcagtttctt taacataaaa acctttattg
     8641 tcaattttaa taagttcttc tttttgcaat acctttatca ttctggaaac agtctccctt
     8701 ttacagccaa gcatatcagc taaatatgta actgtaatat ttacatttag taaagtccat
     8761 tctccacagc aaactccaaa ttctctactt aacctatata attttgctgc aagtttttta
     8821 tctactttta tggatataga atttttcaac tgtctatata aacgtcttat tcttctttcc
     8881 atataagaaa taatatcctt tgtaagttga aaatcatttt gcataatttc taaaaattct
     8941 tcaatactat aataagctac aattgaattt tcaaatgctt cacagccaac agtagagttt
     9001 ttagaagcgt caagtgtaat atcatttatg acttccccac tatttaatat gaaaattact
     9061 tttctctctc cattttcact tatcttaaaa atagatacct ttccttctaa aagtatataa
     9121 acactgtcta agctatcttt ttcataaaat aaaatttctt ttttttgtaa tctttttagt
     9181 ttaacttttt ctttaagttg attcttcgtt ttagtactta aattattaaa aattattact
     9241 ttatcaatat tataatcttt taatttagac atgtaatatc tctccattcc taaattttta
     9301 agagagctct ataaaacatt acaaatttct tattttattc atttttaaac aattttatag
     9361 catttgtagt tttaattttc ctttcaatag cttcattaga aagttctttt actacctgtg
     9421 tatatactaa atcaataatg aaaaattgtg ctaatttaga agatattgaa ccagtttcca
     9481 gtactgtttc tcccgataca taagataagt gtacatctga aatatccttt aattcagaat
     9541 ttttattttc agtaattgat ataattttag cattattggc tcttgcaagc tttacagttt
     9601 ttataatctc ttctgtttca ccagaatgtg aaattgcaat tattaaatct ccttcttcca
     9661 ttatggaaga catcattatc attgtatgac tactgtcaaa acttacacaa ttaagaccta
     9721 ttctcataaa tttatagttt gaatcctgtg ctattatacc agaatatcca atacctatga
     9781 agtaaacttt ctttgcattt attataagcc ttgcgcagtc atgtacatcc ttactattga
     9841 ttatctctac tgtgttctct aaagtggtta catttgaact taaaagtttt tttgctgtat
     9901 ccaaagctgg ctcatcattt tgtatatttt tatttataat atttttttta ttgatagttg
     9961 atatttcttg tgctaaagta actttaaaat cctgtaatcc attaaaattc atttttttta
    10021 caaacctagt tatagttgct tcaccaatat tgctctcttt agctatttgt gatattggtt
    10081 tataaaatac atcatctata ttttctttta tataagctat taataattta tctgattttg
    10141 tgactttaaa agttggtgat tccaattgtt ctaaaatact cattaaaaac ccttctattc
    10201 tttatatttt gtttaaaaaa ttatataatg ctccaagcat tcctgcatca ttttgtctct
    10261 ttgcaaattc tatattagtg tttttgtaaa ctctctctat aagaacctcc tttaatgctt
    10321 tgtctatttt tggttttagg aacttttctt gtgccataat cccaccacct aaaattacca
    10381 cttctggatt tattacataa catatatttg cgattccaac tgctaatgat tttatcatat
    10441 tatcgagttc ttttaaacaa tcttcatcat tattctttgc taaatcaaaa attatttttc
    10501 catttaagtc attttcattc aaatttttta tttttgctac ttttttaatt agactgctag
    10561 tagatgccaa ttcttgaaaa gaacttccat ttacattcat atatcctact tcaccagcac
    10621 tattactaaa cccatttatc agtttattat ttattataat acatccacca attccagtac
    10681 ctattgttaa acatatagaa gatgttgcat ctttacctgc tccaagccac atttcaccaa
    10741 gtccagcaca attaacatca ttttcaactt cacattttat attaaactct ttttctactt
    10801 ctttttttaa ttgcatgccc ttatagtttg gaattaattc ttctaatgca aatagtattt
    10861 taccttcaac tgggtcaacc atacctgcag ttgatataca tataccactt atttcatttt
    10921 cctctatata tttttttact atatctttta ttttggctaa tattccttct ccaccttttt
    10981 cctttgcttc agtatcaaga cttgatttat caattataaa tccatcttct ctaataaaac
    11041 catattttat tgaagtaccc ccaacatcta tacaaacgta atttttcata acatatctcc
    11101 atacaaatta tttttataaa ttaacaactc tatttaaaat tttatattac acaaatcttt
    11161 tacttatctt ttccaattca cagatatatt tccttgcttc tgataagtct atagtatcac
    11221 ttaatacact tttttctttg gttatatgat acttataaat atttttacat aagtcctctc
    11281 ctaacatatt aagaccttct tgagtgtaat gaacatcatc aatagttaat cctctgtcat
    11341 gaaactgcat agcaattttg cttcctacac ataatatatc acattcacta caagccgact
    11401 cctgtgctgc tgcaattcgt cttgttctaa taaaatattc atcttctcca agccaataac
    11461 cagttaaact tacaaacatt ttatcaatca tacattcttc tttaagccta tacgcaattt
    11521 tttttagact ttctttatag tacaatacat cactaccata ttttgcatca ctttcacctt
    11581 gaatccaaat aacgtattta ttttctatat tataatgttt acttaaggta gtacagcctg
    11641 tattaaattt ttctatagtg tcatttagaa accagttatt atcatgattc cagtttttta
    11701 ttcttgaacc atcttttgca ttgtgaataa aacaaacttt atttccagta agcttattcc
    11761 attcatttga aaatgcaggt gctatagtac catcaccttc tgaagtctct agctcacatc
    11821 tcatatttat aatttcatca gtactcaaat attcaaaaca attttcattt ggaatgatgc
    11881 tctcttttgg atttcccaat cctctagcat tgctttgtcc tattaataag aatacatcta
    11941 tattttccat aagttaactc ctttacaaaa ttttaagctt ttaattattt tcaattttct
    12001 cataaaaaac taaggctgaa aatagtaatc taactaaaat ttacgaagtc tcactaatcg
    12061 ctgaaagtta aatcactatt ttcagcccta gtggttatat aaattatcat tatctaacca
    12121 aaaccttaaa aatagctttt ttgatatttt ctttttctaa aacttttatt cctgtcatat
    12181 gagcatcttc cggataacaa actaaaaaat caccttttct aagttccatg tatccattat
    12241 ttttaccatc aagaggcaag aaatcgccct tttcttcata tattttttgt tctaaatttt
    12301 ctataaaatt tatatctatt cgttcacatc catcaagcat caaatgaaca tctatatact
    12361 tcttatgagc ttcccagaat ctatcttctt tttcacaagt tttatactct acaatattca
    12421 caaaaatatc atcaccatct attgtataag tacctttttc ataatctatt aatgaattat
    12481 ctttagcata ttcaaaacat ctaagtaaat cttcatgcaa tgttgaatat gtcttactat
    12541 gatttatatt tccaaatatc ataagcacta tctccctata taattataaa gtctattttt
    12601 atacttttat atacaaagta tattatacaa tcttatagag taactaaatt atttatctaa
    12661 tcactctata agacaattta tcatttaaat attatttttc ataataaaca gtagtctcag
    12721 cttttggtaa agatactttt gctttaactt tagcttctat tgcactaact attgtaccta
    12781 ccactacaga agtcgtgatt gttattagag aatatgccca agaagttact tctggcatac
    12841 ggtattttaa ataaacaact aaaattgctg atactatgaa tccaacatat gctcccattt
    12901 tagtcgcttt tttacaaaat gcaccaagta caaatattcc tgctaaaaca cctagtacta
    12961 gccccataaa actattaaac cattcatatg ctgactttat ttctccattt gctaatacta
    13021 ttgcaacagc tatagaaagt atacctacac ctaatgatat atattgtgct atcttagttt
    13081 gcttttccat acttatatct tttgttatga tagtttgtat atctaaagtc caacttgtag
    13141 caactgaatt aagccctgtc gataaagttg attgagaagc tgcatatatc gctgcaagta
    13201 gtattcctgt tatacccact ggtaattcat aagctatata tgatgcaaat acttggtctt
    13261 gttgaactgt ttgagctaaa tctgggtttt gagtatagta tataaataaa caagttccaa
    13321 ttagataaaa tactgttgca agtcccattg ataaaacacc atttccatat gtcattttat
    13381 taagttgctt tatatcagtt gttgtagtaa atctttgaac tatgtcttga ctcgatacat
    13441 atgatgaaaa agtattaaga ccagctccaa caaatattat aaatgcactt gtagatagta
    13501 tatttttatc aaacataact tcattacttg ctaagaactt gtgtccagtt gtaagagtat
    13561 ccattactgt tccaaaacca ccatttatac ttgctattaa atagaacaat gtccctgtaa
    13621 cacctactat taataccatt ccttgtataa aatcggtcca caagacagcc tttaatccac
    13681 ctgtatatga gtaaattatt gctattacac ccataactat tatcagtaca tttacaggta
    13741 ttcctgtaag accagccaga actattgatg gaagatacat tatgatagac atacgtccta
    13801 tttgatatat tataaacatc aaagctccaa gtactctaag acctttgctt tcgtatctca
    13861 tttctaagta ttcatatgct gtatctattt ctaacctact atatagaggt aagaaaaatt
    13921 ttattgtcaa aggtattgct ataaacatac ctaattgtgc aaaccacaaa atccaagtac
    13981 caccataaga atttcctgct aatgataaaa atgatattgg acttaataat gtggcaaata
    14041 ttgatactga tgtaacccac cacggaatag ttccatcacc tttgaagaac tcttttccct
    14101 tcatttcctt ttttgaaaat aacaatcctg caaataaaac tgccaataag taaaccacta
    14161 gtattgcaaa atctatccaa gtaaatccta ccatttgact aatcctccca tactaaaagt
    14221 attttattaa aaatactttc tgtatatttc ttttgctctt gatttcattt catcagttgc
    14281 ttctttcata ggttgtctac agtatccagc ctcaacacct tgttcttcaa gtagtaattt
    14341 aattgtttga tataagccat ttcctaatat atctgttatt aaatcattag tcacatgttg
    14401 tacttcaagt gcttctgata ttttttcatt cttagttaat tcgaatattt gtctagctct
    14461 gacaccgttt acattgaatg tagaaccaat tgccccatct acacctaaaa ctgtagctgg
    14521 taacatcatt tcatcaaatc ctgcaaatat taatttattt gggaatgttt ttctcattct
    14581 ctctaataag tagaaatctg ctgctgtaaa cttaacacct attatttttt cattttcaaa
    14641 taattctcca aactggtcta aactcatatc aacaccagtt aaaaatggta tagagtatat
    14701 tattaatcta ttatctacag aatttattat tgtattataa tagtgtttta tttcttcaaa
    14761 atcaaattta tagtaaaaag gtgtaacagc acttattgca tcatatccta aatcagttgt
    14821 aaatttagca agttctacag cttcttttaa attaactgaa ccaacttgag ctattaattt
    14881 tatttcttct ttcacttcat ctttagctat ttcaaatatt cttttctttt catcagttga
    14941 aagcatgaag ttttcccctg tacttcctcc tacataaaga ccatctactt tacaaacatc
    15001 tatattgtgt ctaattattt gtcttaaccc tttttcattt atatttcctt ccttatcaaa
    15061 tgaaactaat aatgctgaat aaattccctt catatctgtt gttctcatat ctttacctcc
    15121 aattttaatt ttcaaatata gttaaatata ataatttttt aatttataaa ttaattttta
    15181 attaatttca attgctttaa caaatttttc tgttattaat tgtggtcttg ttattgctga
    15241 acctactact gatgaatgaa cacccaactc aaagaccttt tttaaatctt gaggagtatt
    15301 tacctttcct tcagctatta caggtatttc taaatcttta actaatcttt ccattaatac
    15361 aaaatctgga ccttctaatg ttttagtata tggagtatat ccagatagtg tagttgaaac
    15421 gcaatctaca ccgtactctt gtgctttgat agcctcatca taatttgaaa tatctgccat
    15481 tactagaaca ccgttttctt ttatatactt tataagttct tttaagtctt cattatttgg
    15541 tctaactcta ttggtagcat ctaatgctat catttcacac cctgttgtta aaagttcatc
    15601 aacttctttt ttagttggcg ttatatatat atctgaatct tcatagtttc tctttataat
    15661 acctataaca ggaagtccag taactttttt tatttcaata atatcttcaa ctccttgagc
    15721 tctaattcca acagctccac cttccatagc agccttagcc attctcccca ttataaaagg
    15781 actatgaaga ggttcatctt ctaaagcttg gcatgatact attaatcttc cctttacctt
    15841 atctagcatt ttttaaatta cctcgtttct gaaatttatt ttcttgattt atgtattaat
    15901 aataacatga aaactttttc tttgcaatat tttttcatat ttttttctcg catctatatt
    15961 tttactgatt tattttatat attagcttgt tttttacatt taaaagaaat tttatttcat
    16021 ttttttggtg tttattttca ttttatataa ctactgttat aagtccattt aaataatttc
    16081 aataaaaaaa cccatgtata ctatacatag gtttcccaat attattctaa atccattata
    16141 atttttcttc cacagcatgg acaaaattca gcctctgtat ctatctcact ttcacaataa
    16201 ccacaaatta taaaatcatc acattcattt tccaattcaa attttttacc acattcagca
    16261 caatatttat catttacatt tccaacataa ccacactcac aagttacatt actttctgcc
    16321 atttccattt cattaatttc catatttttt tcataaatta acttatcaag ttctagtaac
    16381 tcatcacaga atttatcaaa actattatca ttaatagaat cttctctaat tttttgatat
    16441 gtcagtattc ccatttccaa gaatatcttt gctttctttt cttgaatatc atcaatctct
    16501 ctatttaatt ttgatttttc tttatataat tcaactttaa attttccact accaaacttt
    16561 ttcttaacct tagaaaattt atcatctact ttattattag tatttccttc cacagaaatt
    16621 ccctccaatc atatttaatc agctgaattt agcttatatc ctattaaaga tatcacaaaa
    16681 ctatatacaa atgaaattac aatagcttgt aagactttaa attgcattat taaagttgtt
    16741 ccataattag ttgtattaaa gaaattaata ttactatcta gtataaatgt actaaataaa
    16801 gccaatattc ccatcaaaaa tgcataatat atactaaata ctattatagg tcttatacta
    16861 tctgtattat acttaaatct tagcttgtat ccatttaata ataatattaa catagacaat
    16921 gctcccattg cataaaacat taatttagta tctccaaata aagatgaaga acttaaagta
    16981 aacattgata ctgttgagtt tattatggtt actgaaattc catttgcaaa agcccacata
    17041 taactagcta tttgtgggag tatagtaaat aaatttaatc catttgaata actactcata
    17101 tctagttcat ttaagtaact actatcagat atagttagta ccaatagtat tactaatact
    17161 aaaacataac ctaatatgaa tgttcttata gaatttgaaa aaagagataa atacatattt
    17221 tccttttcat aacttttttt ataagtagtt atgtatgtac atataaaccc taatacaaat
    17281 ccatttaaaa gaacactaaa aaattcataa ctatattcta atgcatatcc atattctaac
    17341 atactatgtg aactagtttt tacattagta aaaatagata aaatagctaa aactaatcca
    17401 taaaaaattc caactcctag agaatttgct aatacagttt ttgaatctct acatcttttt
    17461 ctcataaaaa ttaggtttga tattaataat accaaaatag ggattataag taatatcaac
    17521 aaacctatat tggcatttat aaacccagaa cccatcatgg tagacataga tacactaatt
    17581 tgacccaagt tgagagctag gattatatgt gctggattta ttaaataact aatatcatta
    17641 aattgaatag ttataatagc tttaaatatc aatgcaataa taaacaatat aaatatggtt
    17701 gtaaatgttg ttaaaaatac acctctttta tttgcatgat atgaaagctc ttttatttta
    17761 gatttcaagt ctagttttgt ttcaagtggt cttaatgatg ctatttcata aagttcattt
    17821 ccacaaaatt tacagtaatt atccttagta ttattttctt tattgcaact agaacaaatt
    17881 ttttcttctt tacttttaag agtgatatgg ctatttttta tttttctagt tggttcacct
    17941 gtctttttgg aataattatt atgtgaataa ttcatattat ttattttctt ttcacccatt
    18001 ttttccctcc aagttttact attgtaaatt atcaaatata actatatcat ataaaactta
    18061 attatacctt aattggatat atttatctat tttatttgtt tattgctgtt gttttttaac
    18121 aggtcttgat atttaaatag tttgttaatg cgaactttat tatttatatt ttattatgct
    18181 taatttaaat ttattctatt ctttattttc agtagtaaac tatctgtaaa tcttaattat
    18241 aataaagtat taatcaataa agttactcga cattatctaa taaattttat ctatcacaat
    18301 tttagattgt tacacacaat atattttgat ataattaaat cataaacgaa gtattccaaa
    18361 tatctaatga tagaaaggaa tacttatttt tatgctttat ttttaatgga ggcttatatg
    18421 aacttagaaa aaataaaaaa attttttgga tttcctctag acaaaaaaca caaagacaat
    18481 gttgagaaag aactctgcaa ttatctttat catacgggta aaatcatttg cataataata
    18541 gtaatatctc aaataattat gatgatatca atctcagcta gagaaggagg cccttttgtc
    18601 acaccaagac gccaatggta tttttatatg tatgtaacac tattttgtgt atcttctgtc
    18661 tttatgtttg tattttctta cctttggaat aaaaaaaagg attgctgtac gttttatata
    18721 aatttatgga taatttatgt gacttttttc tgcctatggg gttgtgtaat tactctactt
    18781 gaccagttag gtggaaatga cctaagtgta tttagttata ttatgttagc agctgcagct
    18841 ttctcagtat taaaatttgg agaatcagta atagtttttg gaggatgctt tttgtttctt
    18901 aatttattct tacctgggat tcaaactata ccaggcaatg agtttagtaa tattgtcaac
    18961 acatttttta taacatccct tgccattttt gtttctacaa tgctatatag aaatcgtgta
    19021 tttacttact atgataaact tttaattagt caacaatatg aagaaatatc tcatataaat
    19081 aatttactta acgacaaagt tatgacagat gaccttacta aaatgaacaa ccgtaggtac
    19141 cttgaagata ttctacaaaa aaaattaatc agccatgcaa atcaagaacc tataactgga
    19201 atgatgatag atatagatta ttttaaacaa tttaatgata attatggaca tcaaaaggga
    19261 gactattgtc tacaaaatat tgccaaagta attttagatt ttacaagttc tgaaaatgaa
    19321 catgctatac gatatggtgg tgaggaattt tttatatgta tttttaactg taatgaggca
    19381 gatgctaaac ttaaatcaga atgtttacga aaaattatag agtcctgcaa ttttgagcgt
    19441 gatgatgttc ctttaaaatg tgtaactgtt agtattggag tttatacaaa gaaaattgga
    19501 aagacaataa cattaaatga gtttataagt aattgtgata aggccctata tactgcaaaa
    19561 gaacaaggac gtaattgtgt tgcattttat taaaaggtta catactatta cataaaccta
    19621 taagtattat atcgcaaaat aaaagtaatc aaattatata tttatagtac aataaaaata
    19681 acttgcatat ctctaaaata gataaatatg caagtcattt tagtgtttat acttaaagtt
    19741 atataagttt tattatatat aattattaaa attcagcatt tttaggagtt cttgggaaag
    19801 gtataacgtc tcttatgtta gacatacctg tcatatacat aattattctt tcaaatccaa
    19861 gaccaaatcc agagtgagta gcagtaccaa actttctaag ttctaaatac caccaataat
    19921 cttcttctct taatcccatt tcattaattc tatctaacaa tacatcttct ctttcttctc
    19981 tttgagaacc tcctacgatt tctccaactc caggaactag taaatccatt gctctaacag
    20041 ttttaccatc ttcattcatt ctcatataga aagcttttat atcttttgga taatctgtaa
    20101 caaacactgg acaattatat atttgctccg ttatatatct ttcatgctct gtttgaaggt
    20161 cacatcccca ctcaacagga tactcaaatt catgcccaga ttcaattaat aattccactg
    20221 ctttagtata cgttattcta ataaactcag agtttactat cttatttaat ctttctaaca
    20281 atcctttatc tataaaacta ttaaaaaatt ccatttcttc aggagcattt tctaatacat
    20341 agcttataac atattttatc atatcttctg caagttccat gttatcttca aggtctgcaa
    20401 aaactatttc tggctctatc atccaaaatt ctgaagcatg tcttcctgta taagagtttt
    20461 cagctctaaa tgtaggtcca aaagtataaa cgtttctaaa agaaagtgcc attatctcgg
    20521 catttaattg accactaact gttaaattag cctcttttcc aaagaaatct tgagtatagt
    20581 ctattttgcc atcttcagtt tttggaatat catttaaatc catagttgtt attctaaaca
    20641 tctctcctgc accttcacag tcactacctg ttatgattgg agaatgtgca tatacaaagt
    20701 ttctttcttg gaagaatttg tgtattgcat aggcagctat tgatcttact ctaaatacag
    20761 ctgaaaaagt attacttctt ggtcttaaat gtgctatcgt tcttaaatat tcaagcgtat
    20821 gtctcttctt ttgtagtgga tacgaactat ctgattcccc ttctacaact atgctttttg
    20881 catgtatttc gactggttgt ttagcccctt ctgtagatac taactctccc tctacaagta
    20941 tagatgagct tattgctaat tttcctacct ctttaaaatt ttctactttc tcatcaaata
    21001 ctatctgcat atttttaaag aagcttccat catttagctc tataaatcca aaatttttag
    21061 atactctaga tgttcttatc catccagcta cttttacagt cttccctatg tactgttctt
    21121 tttctctgta caattctttt actgttataa attccttttg cattttaaat cctcctagta
    21181 taattgttat taatttcttt taaatataat ttcaataaaa aaagcccttc atccctatat
    21241 aaagggacga aagactatta ctctcgcggt accaccctag ttgtcataat gacctcttag
    21301 ttaagcaatg cttaaagcct ataacggtgc cttccgtcta agtctactta ctaaataagc
    21361 tttcgattag aaactccaag atgttcttca atataaacct cgtattgagc tttcaccatc
    21421 ctcaactcgc tagaactaca ttttatatct actcttctct tcacagtatt tatcttttat
    21481 tatttctgct aattaacaaa attataccaa tttataaatt tttagtcaat aaatttatat
    21541 aattgatact gattgttatt taaaatataa taattctacc ttacataatg ataataaata
    21601 cttatcttca ttacttcttt attacttcaa tttccaaaat tttataattt acttccattg
    21661 tcaacccagt cttttgcttc ttcgacaccc ttaattgaag gtattgaaac tttactatga
    21721 ggttttaatt tttcaacatc agcccatgct gctgtcgact catttccaac taaaagtgaa
    21781 cttttaagtc ttttattatc tttacctttt aatgctcttg cctttttact ttccaaaact
    21841 atcacctcta ttatttattt tgtaatagtt ttagcaattt tacaaataaa attcataagt
    21901 ctaaattata attattttta aattaacata atatcttttc aatacttaat ttatacctat
    21961 ttatatacaa aatatttact ttgtgtattc aaaatataaa actgtttttt aattttcata
    22021 tttatttttt atgctatttt tctactctat ctaaagtatt tgatacaatt aaactaataa
    22081 tcaattataa ttttacatag gttcttatct atagagtatt tgctatctgt tgaatcgtat
    22141 tttttaaatt taaaatccca tatccctgcg atacatttgg atatgtatat atctcaagtt
    22201 ttgtagcacc caatattaaa tatgttttta aaacttgtgt aaacaatgat aatctcggta
    22261 cattatcctg tttttctaag tattccatca ataaggctaa aacacctgtg actatagaac
    22321 tgctcactcc tgttcctgtt cctgtattat atgttccatt tttatatgtg gatattatat
    22381 ctacacctga agctactata tctggcttaa ttcctcttcc tctaattggt cctttcgaag
    22441 aaccaatcca catactatca gtcttattgt taaatgtacc aactgtaatt acatcatcac
    22501 tagcagcata catagttata gtagctactg aatcaggctc taaaaacctt gtgtctggag
    22561 ctatcaaatt tttatttggc aagtatatgt catattctcc atttattata agttctgaag
    22621 tcaatcttaa aatccatact ccaggtttta tatctcttaa tcttatctcc aaattttctt
    22681 ttccagaggt aatatatgga taaataaatc tcattgcata agtagtattt tctaagttaa
    22741 attttcctct atatatataa aagtctggag aataccttat atcatgacta acttcacctg
    22801 aaggagatat tatctgtgct cccactttat ctggaccatt cgtatttagt gttatatcaa
    22861 gagcatagtc atctccatct tgtattatta catcttgaac ttctcccaca ctgctaaatc
    22921 tgccagaata atgaatatct gtattccctt gatttcctgc accactcact actaccacac
    22981 cagcactact caatatatta aaagtatcta gtattgaagt agttgctaca gcacttgact
    23041 taactccaat agtcaaattt attatcagtg gtttattttc tgttcttgct atgttagtaa
    23101 catacgttat agctgctaaa aaatctgaaa cactgtaatt tatccttcca gcataataag
    23161 tatctgtata tgattttaat ttaactacta ttaagtctga ttctgtagta atacctctgt
    23221 actgagaatt tatccttcca tttccaacta atataccaga aactaaagtt ccagtaccta
    23281 tattatcttg actcaaactc ccatcatttc tatttatggc tatatttaac tcacttcttg
    23341 taaattcact tccaaaaata aaaccttctg gaggtggatt tgtatttgct tcttggtccc
    23401 ataaatataa aactttacta gttccatcat catttataaa atcaggatgc aaataatcta
    23461 tcccagaatc tataactgct aataaaattc ctctccctgt tatatcatta tatggatttt
    23521 cataaatata atcagtttca gctgctgttg taatggtttc tccattattt acattattag
    23581 ttatttcaat tagactgctc attggttcag attcttccca ccaagcaact tgtattatat
    23641 tatttaaaat agtttcatca aaatctaaag gtacatatat tactgcaagc tgactattta
    23701 aaaccatata cctatttatt ccattttctt gtagggcaga ttctatatca ccttgataaa
    23761 ttatacaata agatttttcc ataaatccct cctatcttaa aacatcaaac attcctctaa
    23821 catccaagag cccatatcca gaatttgtat ttggatatac tgtgtttgaa tcttttctag
    23881 caccagcttg cataaaagtt tttatttttt gcacatatgc ttggtttgga tatctaccgt
    23941 caacaaaagt atattgaaaa tacattgcag cagcaccagc tgcatgtgca cttgctgctg
    24001 ctgtaccagt tatagttgca tacgtgttcc caggataagc agctattata ttgacacctg
    24061 gtgctactat atctggtttt aacctgtctt caatagttgg ccctcttgat gaactttgcc
    24121 ataaactacc attaatggta ttgtaggctc ctatggttat taaatcatct tgaatcgcag
    24181 gataatttat agtgtaaaaa gggtctacct ctctaaatct agttccactt tttaaaagtt
    24241 ctctatttgg aaggtataag ttatatcttc ctgttataat ataaactcct actaatcgaa
    24301 tcttccatac acctcttttt gcattcttta aagtaacatt agtaaattgc tgtccagaaa
    24361 aagttgttgg gtatatatat gttatagaat attcagtccc ctctaaatca aataaacctg
    24421 ttactttatt ataatttgaa attccaacac ttttactttc ttcaccagtg ggagatacta
    24481 ttattacatc agctttatct ggtctattta accaaagttc taatgataat tcttcctcgt
    24541 cttcatttaa ctctaattca acttcaacag aacctccaac atatggtatt attccagatg
    24601 tatgtgtctg tgtattgccc tcatttcctg ctcctgccgt tatacataac cctctagtaa
    24661 agaacgcttt ttcactatta ctcctattgg ttaaacctgc taggctactg gtacctaaag
    24721 acatatttat aactaatggt cttccaagtt caaaagcttt tttatatgca tactgagatg
    24781 cagcaaacaa cattgcacta ttataaaaat catctatctt gccgagtttt attattatta
    24841 gctcagaatc ttctgctata cctgcatatt cactatttac atttccaagc cctgcacata
    24901 tcccactcaa catagttcct tgaccaactt catcctgaga tagactaggg tcattttctg
    24961 cgatagctcg atttatatct tctcgtgtat attctgtccc aatataaaaa ccatcaggag
    25021 gagttccttc ttttgtttgg tcccataaat atactatttt tgaagtgcca tctggataaa
    25081 taaaatctgg gtgtagatag tctattcctg tatctgcaat tgatattaga gtccctcttc
    25141 ctgttatatt aatattagga ttatttttaa agaaatttac gcccatttct tctgttgcaa
    25201 ctacacctcc aaaagtacct cgattaattt ctccaagtaa tgtcatcttt gtagtcgata
    25261 cagttcttat gattgaagga agttgcaaaa ctcgttgtat gctattatag tctgtattat
    25321 ttatagatag tacacctaat gaatcactta tcttaaaaaa cctttgactc atacctaacc
    25381 cagctagttc ttcttcaaat tcaggagtat ggactatatc tattcctgat agtattcctg
    25441 attgtctact tgcattttta cttatataaa tttcattctc taagtcatta tgtatttgca
    25501 tattataacc atctataaac ttataactat tttcctcatc tttaaatgac ttattaatac
    25561 ttctatatcc aatatcctct gtttctaaat cttgatttat attagataga gtgtacaaat
    25621 ctatatctga taaatttaaa aaaccaaatc ctgatgaatt atttggataa gattgattac
    25681 ttaatctcct tgctcccttt agaagtaatg cccttaattt ctgtgaatac aaaaataaat
    25741 catttccatt tacaattccc cattccataa acaatgaaca aacccctgta acatgaggtg
    25801 tagccatgct agttccagtt aatgctccac ttgtacctcc tggtaaaaaa gatactatat
    25861 cttcacctgg agctaataaa tcaggtttaa aaacacctaa ttgtgtatct ccttcaccag
    25921 aaaatataga tactatatct gttcttgaat taaaactacc tactgttata actctacttg
    25981 ctgttccagg aactgttaca gtaagctctt gagtaggaat cagaaatcta gtatttctat
    26041 ttagaccttc agaagttggc aaatatatat ttacattacc agttactata tctattggtt
    26101 caaatacaat tttccataac ccaggagtta tctgtgtatt tgaacttaat tgtagagtaa
    26161 ctcttcttgt gagagaatac ggtgctatag ggtaaaaata accagttatt ctagtttcac
    26221 ccaaagtatt ccttatctca cctgaagtta gagagattgc ttgtgtttga ttatttgatg
    26281 ggtttaccaa atgtacacta aaatcatcaa caaaatctgg ccatatattt atgtttaata
    26341 tacgctctcc ttccccaact ataaactcaa cttcttctgt aatattattt tgaagcctaa
    26401 tccttttatg acctccttta tctgcattat ttccagctgc tacaactata ttgtttttcc
    26461 aaaataaaca catatcatct atatattgtt caaataaaga tgttcctcta tgtgaacctt
    26521 cattacttcc ataacttata tttaatgtaa caggcattct taattctaat gccctatcta
    26581 aaataaattt aattgccctc ataaactctg tacttctcga aaaaatatcg gtctgtatat
    26641 ttccaactct aaccactatt attctggcat cacttgcaat agttgcacaa atacctgcaa
    26701 catgtgttcc atgtaagcta gttgttgaaa taggtatata catacttcca tctattgcat
    26761 tatttatatc ttcattagta tataatgttc cttctctaaa accttctggt ggatttcctt
    26821 gaatagattg gtcccaataa tataatatct tagaccttcc atcactatct ctaaacacag
    26881 gtagagtata atctattccc gaatcaatta taccaattat agttcctttt ccagttaaac
    26941 cagttctatt tttaaatcct gttattcctg tacttgaaaa actttgcaca tcctgagtct
    27001 gtaatataaa aggcttttct ataaattcaa tttctggata agttaaaagt atatttacat
    27061 cttcttcatt acttgatgtt attattgcat atgatgaatt aagtatctct actgatacac
    27121 ctaattcttc ttctaacctc aatatatctc cattgtattt tacaattaat tcataattta
    27181 ttataatcac ctcgacttct aaaattatta ttaatgtata tgttttgtgt gagtctaaaa
    27241 tgtattagac tatataattt tttaagtttc agttatacaa tttataatag atatattaaa
    27301 aaaataaata atgatggtac aattttgaat aaatcatacc atcatttata tttactttat
    27361 cttgaaacca ttaaattttt caatgctttc tcaagacctt ttacagtcaa tggaaacata
    27421 ttaaattcac tttgtatcat tctttgagtt tgataccaat aactaattga atcccatcca
    27481 tctctaagtt ctggattcat ccatacaatt ttactgtact ttctcttaaa ccttttaagc
    27541 cactctatac caggtgtata attatatgga ccacgatagt taccaccaat atgaagcaac
    27601 tcagaaggcg acatagaagc atctccaaca attataactt tatagttttt atctatattt
    27661 tttaatatcc attcagtatt aatagattta cttaaccaac attcaggtgt tgtaaataat
    27721 ctatcataaa tacagttatg gaaataatat atctttacat ctttaaaatg atttgattta
    27781 cttacagact gaaataatgt attacataaa gtgctatacc ctctcattga accacctgaa
    27841 tccataagca ataaaagctt cactgtattt tttcttggct tctcaaaaaa taactttaaa
    27901 tatcctgcat tattacaagt ctcttcaata gtcttatcca aatcaagctc tgtctttgga
    27961 gcatctattc tagtagaaaa ttgacgaagt ctctttagtg caacttgaaa ttgacgcata
    28021 tttaaaactt catcatctct aaaatctctg tattttcttt ctcctgcaac ttcaagaaca
    28081 gctctaccat aagtagaatt tccacctata cgtataccag tttgcccttt tcctgaatgt
    28141 cctaactctg aagtgcctcc tgtgcctatc caataattac caccattatg aggagaatct
    28201 tgttctttta ttcgttcctc caatttagct cttaatttat caagatttat atttggattt
    28261 tgttctaatc tttcaaactc ttctaaatct atatctggtt ttttaagcca attcataatc
    28321 tcttctggaa tttcttcttg gtgttctata cctttaaaat actccaaaaa cactgcatca
    28381 aacttatcaa aatcactttc actttttatt aaaatcatcc tgcaaagata gtaaaaattt
    28441 gaaaagtttg aatagcacag tcctttatcc aatgcttcaa ttaaagtcat ccattcattt
    28501 aaagatacat ttaatcctct tgacctcaat aaataaaaaa attcaataaa cataattatc
    28561 cagctcctat taactataag tgtctttgca tagattcaat atcctcatta ttcttaagta
    28621 gtattcctgc aaaaggaact tctttttcta ttttttcaat aggcattcca ctcaaagtta
    28681 atgcttgtat ccagtctatt aactctgatg tactaggctt tttttgtatg tcctttaaac
    28741 ttctaatcca ataaaaagta gtcattactt gttccaatag gtgttcttct actttatcaa
    28801 aatgtacttt aactatttct tccatcatgt ctctatcagg aaattctata taatgaaata
    28861 tgcatcttct taaaaaagca tctggaagtt ctttttccgc atttgaagtt ataataacta
    28921 ttggtctttg tttagctctt acagtttctt ttgtctcatt tatgtaaaac tccattttat
    28981 ccaattccca aagcaagtca tttggaaatt ctaaatccgc cttatctatt tcatctatta
    29041 aaagaattac ttgttgattt gagctaaagg cctctcctag tttaccatat tttatgtact
    29101 tgctaatatc atctacacct tctcctccaa attggctatc ataaagtctt tgaacagtat
    29161 catatacata aagcccttct tgtgcttttg tagttgattt tatattccaa attactaaat
    29221 ctttttttaa ttcatttgaa attgcttgtg ctagcatagt tttacctgtt ccaggttcgc
    29281 ccttaatcaa aagtggcttt tcaagagcaa tagcaacatt aactgatgcc atcaattctg
    29341 gtgatactac ataatcacta cttcctctaa agttagatat ctcttttttt atcataaatc
    29401 ctactccttt taaacagtat tttctaaatg tatatagact ttattactat ttttgatata
    29461 aaaataataa tacaatacat ttgtatagta ttattctaaa attctatatc taaataaaat
    29521 cacctctaaa atatttaatt tttatcttta tttgtcataa ttaaatgaaa tatatatttt
    29581 aaagaataat aaaagatagc ttattgtaca aaatttaaat tataaaaact tagtttaatc
    29641 aaaggcacca ctttttttca
//
LOCUS       DAYATN010000036        29237 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.036, whole genome shotgun sequence.
ACCESSION   DAYATN010000036 DAYATN010000000
VERSION     DAYATN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.036"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..907
                     /gene="dprA"
                     /locus_tag="ACGI8A_001569"
     CDS             <1..907
                     /gene="dprA"
                     /locus_tag="ACGI8A_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFH0179570.1"
                     /translation="ENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLFYKGDIGL
                     VNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHIGCMSGKS
                     KTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRNRIISGLS
                     DGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGAKLIENID
                     DILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYTGIEIKYV
                     NSIINELVLNELVVEMNNKTYSLNV"
     gene            940..3027
                     /gene="topA"
                     /locus_tag="ACGI8A_001570"
     CDS             940..3027
                     /gene="topA"
                     /locus_tag="ACGI8A_001570"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFH0179571.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNIGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            3192..3977
                     /gene="codY"
                     /locus_tag="ACGI8A_001571"
     CDS             3192..3977
                     /gene="codY"
                     /locus_tag="ACGI8A_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFH0179572.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            4084..5349
                     /locus_tag="ACGI8A_001572"
     CDS             4084..5349
                     /locus_tag="ACGI8A_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFH0179573.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            5380..6150
                     /locus_tag="ACGI8A_001573"
     CDS             5380..6150
                     /locus_tag="ACGI8A_001573"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0179574.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            6259..6705
                     /locus_tag="ACGI8A_001574"
     CDS             6259..6705
                     /locus_tag="ACGI8A_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFH0179575.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            6723..7916
                     /gene="nifS"
                     /locus_tag="ACGI8A_001575"
     CDS             6723..7916
                     /gene="nifS"
                     /locus_tag="ACGI8A_001575"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HFH0179576.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            7970..8410
                     /gene="nifU"
                     /locus_tag="ACGI8A_001576"
     CDS             7970..8410
                     /gene="nifU"
                     /locus_tag="ACGI8A_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HFH0179577.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            8699..9778
                     /gene="mnmA"
                     /locus_tag="ACGI8A_001577"
     CDS             8699..9778
                     /gene="mnmA"
                     /locus_tag="ACGI8A_001577"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFH0179578.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    9921..10159
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10227..12866
                     /gene="alaS"
                     /locus_tag="ACGI8A_001578"
     CDS             10227..12866
                     /gene="alaS"
                     /locus_tag="ACGI8A_001578"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFH0179579.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            12891..13148
                     /locus_tag="ACGI8A_001579"
     CDS             12891..13148
                     /locus_tag="ACGI8A_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFH0179580.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            13158..15329
                     /locus_tag="ACGI8A_001580"
     CDS             13158..15329
                     /locus_tag="ACGI8A_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179581.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDIMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            15535..15960
                     /gene="ruvX"
                     /locus_tag="ACGI8A_001581"
     CDS             15535..15960
                     /gene="ruvX"
                     /locus_tag="ACGI8A_001581"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFH0179582.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            16038..16325
                     /locus_tag="ACGI8A_001582"
     CDS             16038..16325
                     /locus_tag="ACGI8A_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFH0179583.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            16607..17065
                     /locus_tag="ACGI8A_001583"
     CDS             16607..17065
                     /locus_tag="ACGI8A_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFH0179584.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(17182..17919)
                     /locus_tag="ACGI8A_001584"
     CDS             complement(17182..17919)
                     /locus_tag="ACGI8A_001584"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFH0179585.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            18136..19812
                     /locus_tag="ACGI8A_001585"
     CDS             18136..19812
                     /locus_tag="ACGI8A_001585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFH0179586.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            20018..20218
                     /locus_tag="ACGI8A_001586"
     CDS             20018..20218
                     /locus_tag="ACGI8A_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1290 family small acid-soluble spore protein"
                     /protein_id="HFH0179587.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            20272..21435
                     /locus_tag="ACGI8A_001587"
     CDS             20272..21435
                     /locus_tag="ACGI8A_001587"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFH0179588.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(21646..22068)
                     /locus_tag="ACGI8A_001588"
     CDS             complement(21646..22068)
                     /locus_tag="ACGI8A_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HFH0179589.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(22089..22925)
                     /locus_tag="ACGI8A_001589"
     CDS             complement(22089..22925)
                     /locus_tag="ACGI8A_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFH0179590.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            23223..23819
                     /locus_tag="ACGI8A_001590"
     CDS             23223..23819
                     /locus_tag="ACGI8A_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFH0179591.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            23849..24568
                     /locus_tag="ACGI8A_001591"
     CDS             23849..24568
                     /locus_tag="ACGI8A_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFH0179592.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            24570..25109
                     /gene="scpB"
                     /locus_tag="ACGI8A_001592"
     CDS             24570..25109
                     /gene="scpB"
                     /locus_tag="ACGI8A_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFH0179593.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            25271..25900
                     /locus_tag="ACGI8A_001593"
     CDS             25271..25900
                     /locus_tag="ACGI8A_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HFH0179594.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            25884..26282
                     /locus_tag="ACGI8A_001594"
     CDS             25884..26282
                     /locus_tag="ACGI8A_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HFH0179595.1"
                     /translation="METTLETIKGSIDANTIIGDPIKTDTTVVVPISKVTIGFGIGGG
                     EYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETRIMSLDSNINL
                     VDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            26530..26970
                     /locus_tag="ACGI8A_001595"
     CDS             26530..26970
                     /locus_tag="ACGI8A_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0179596.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDINNKDKLFTSLEESKNIIEQLF"
     gene            26986..28437
                     /locus_tag="ACGI8A_001596"
     CDS             26986..28437
                     /locus_tag="ACGI8A_001596"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFH0179597.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALILLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(28528..29196)
                     /locus_tag="ACGI8A_001597"
     CDS             complement(28528..29196)
                     /locus_tag="ACGI8A_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HFH0179598.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT        11817 a         3035 c         5246 g         9139 t
ORIGIN      
        1 agaaaatata aaagaactac tttacaaaaa agcgattaaa tatatttgta aatatgataa
       61 agaatatcct gaaaatttaa agaatatata caacgctcca aagttattat tctacaaagg
      121 tgacataggc ttagttaata ataattttaa tatagctata gtaggttcaa gaaaacctac
      181 agcctatgga attaattgtg caaaaacaat aagttgtcaa ctatcccaat atggagttaa
      241 tatagtaagt ggattagcta taggaataga tgcatactcg cacataggtt gtatgagtgg
      301 aaaatctaaa actatagcag tacttggttc aggagtagac aatcctcttc caaaacaaaa
      361 tttacattta tcaaataaaa tattagaaaa tgggggatta ttattatcag agtataatat
      421 taactcgaca gtagccccgt accatttttc taatagaaat agaattatca gtggtttaag
      481 tgatggagta gttgttgtag aagcagcaat aaaaagtggt gcactaatca cagtagattt
      541 tgctcttgaa catggaaaaa atgtttttgc aatacctggt aatataaatt ctcaaatgag
      601 tagaggttgt cataaaatta taaaagaagg tgcaaaatta attgaaaata tagatgatat
      661 tttaaatgaa tataacatat ttaatattat tgataaaaaa ataaatcaaa aatatgataa
      721 tataagtcta aatgccaagt ctaagcaaat aattgaagct ataaaaagag aaggaaactt
      781 gcatatagat agtatttgtg attatactgg tatagaaata aagtatgtaa attcaataat
      841 caatgaatta gtactaaatg agttagtagt agaaatgaat aataaaacgt acagtttaaa
      901 tgtataaatt aagattaggt agtgggggtg tatggtttta tggccaaaac attagtcatc
      961 gtagaatcgc ctgcaaaagc taaaactata gaaaaatttt tagggaaaag tcattacaca
     1021 gttaaagcat cagttggtca tgtcagagat ttacctaaaa gtaaattagg tgtagatata
     1081 gaaaataatt ttgaacctca atatataaat ataagaggaa aaggcgatgt aataaaagaa
     1141 ttaaaaaaag aagctaaaaa atctaaacaa gtatatctag caactgaccc tgatagagaa
     1201 ggtgaagcta tatcatggca tttagctcat atactaaatt tagatgaatc tgaggattgt
     1261 agaatagagt ttaatgagat aactaaggat gcaattaaaa aagcaattaa acatcctaga
     1321 aatattgata taaatttggt tgatgcacaa caggcaagaa gagtgctaga cagactttta
     1381 ggttatcaaa taagtcctat attatggcaa aaggttagaa aaggtcttag tgcaggtaga
     1441 gtgcaatctg ttacaacaaa gttaatttgt gatagagaaa aagaaataaa ggcatttatt
     1501 ccaaaagaat actggacaat agatgttgag gctaagacag taaaagacaa agatataact
     1561 ttgaaatttt atggtaaaaa tgatgaaaag attgaattag aaaatgaaga agctgtaaat
     1621 gaaatactaa aagaaatgga aggtaaagat ttaaaggttg agaagataga atctaaatca
     1681 agacgtaaat cagctccaaa accatttaca acaagtatgc ttcaacaaga aggagcaaat
     1741 aagttatttt ttactactaa aaaaacaatg atgatagcac aagaattata tgaaggtatc
     1801 gacatagaag gtgaaggtac tgttggtctt atatcatata taagaactga ctctaaaaga
     1861 atatctgatg aagcaaaaga aaaagctaag ggttatatcg taaacaattt aggagaaaac
     1921 tactataaaa acattggtga taaaaaagaa aaaaaggata ctaaaaaggt tcaagatgcc
     1981 catgaggcaa taagaccaac ctcagttgat agaactcctg agagtataaa agcctcattg
     2041 agcaaagacc agtacaagtt atacaattta atttggagaa gatttgtagc tagtcaaatg
     2101 gaagattcag tgtttgatat actaaatgta gaatgtaaaa taggcgatat tgtatttaag
     2161 gcaactggtt caaaaatgaa atttgatggt tataccaagg tatataattt ttcagaaaga
     2221 gaagacaaaa ttttaccaag tatagaaaaa ggagatatat taaatataga aaatatattg
     2281 ccagaccaac attttacaca accaccagca agatatacag aagctagttt agtaaaaaca
     2341 ctagaagaac taggtatagg tagaccaagt acgtatgctc caacaatagc tactatactg
     2401 aatagagagt atgttgaaaa gcaaggaaca agtctatgtc caacagaact tggaataata
     2461 gttactgata ttttggaaaa taatttccat aaatttatag atgtagattt tactgctgat
     2521 atggaaagta aacttgatgt tatagaagaa ggcaatacag attggaaaaa gattgtatca
     2581 gaatcatatg caccacttaa agaggctatt gaagaagctc ttgaaaatat agaaaaagta
     2641 aatatggatg aagaaactga tgagatttgc gagaactgtg gttctaatat ggttatcaaa
     2701 tatggtagat ttggaaagtt tatggcttgt aaaaactatc ccgattgtaa aacaactaga
     2761 ccaattatga ataaaattgg tgttaaatgt ccaaaatgtg aagaaggaga tattatactt
     2821 agaaaatcta aaagaggtaa agctttttat gggtgttcta attatccaaa ctgtgacttt
     2881 gtagcttgga ataagccaac aggagagttt tgtgagaagt gtggttcata tatggttgaa
     2941 aaagtgacta aatctgaaac aaaaacagtg tgttctaata aagaatgtaa taaagagaca
     3001 aaagagcata atgacggaaa agagtgaaaa ttgaaaaaaa tcacataatt ctcttgaaaa
     3061 aggttccaat ctatgataat ctttcaagtg taaacaacta atagaaaaat aaattttcat
     3121 taaatgtgaa aataatatta gaaaatataa aaggttatta aaaggcctaa atttcatgag
     3181 gagatgatta aatggcaagt gaagtgttac aaaaaacaag gaaaataaat aaaacattac
     3241 aaacaagtgg tggaagcagt gtctcttttg atttactggc cggagcattg ggcgacgttt
     3301 taagttctaa tgtttatgta gtaagtgcaa aaggtaaagt actaggtctt catttaaatg
     3361 atgttcaaga cagttcagtt atagaagatg agtatactaa gcaaaagaaa ttttcagatg
     3421 aatatactca aaatgtgtta aaaattgatg aaacattaga aaatttaaat ggtgagaaga
     3481 tattagaaat ctttcctgaa gaacatggaa gattacaaaa atatactaca gtagttccaa
     3541 tattaggaag cggtcaaaga ttaggaacat tggtactttc aagatattca aattcattca
     3601 atgatgatga tttagtaata gctgaataca gtgcaactgt tgttggtctt gaaatattaa
     3661 gagcaatagg tgaggaattg gaagaagaaa tgagaaagaa agctgtagtt caaatggcaa
     3721 taggcactct gtcctactcc gagcttgaag cagttgaaca tatttttgct gaattggatg
     3781 gaaaagaagg tttacttgta gcaagtaaga tagctgatag agttggtata actaggtctg
     3841 taatagtaaa tgcacttaga aaatttgaga gtgcaggtgt gatagaatca agatcattag
     3901 gtatgaaagg tactcatata agaatactta atgacaaact tacagatgaa ttaaaaaaat
     3961 taaaaaacaa tcaataatct atattttata ggtttagatt agaaaaggta tctatttcaa
     4021 atagatacct tttttagacg aattattaag gtaatgataa gtatattaaa aaggagtgag
     4081 aaaatgcctt tagcagataa aataagacca aaaacaatga atgatgtaat aggtcaaagt
     4141 catataatag gtaatggaaa aatattgtct aagatattac aatctaattt tttaccaaat
     4201 atgatatttt ttggtcctcc tggtgttggt aaaactacag tagcggaaat aatagcagaa
     4261 agaagcaata aaagcttcta taaaataaat gctactaatt catctttaga agatataaaa
     4321 aaggtgatat cagagcttgg aagtataaat aatgtaaatg gagtcctatt atatatagat
     4381 gaaatacaaa gttttaataa aaaacagcaa caatcaatat tagaatttat ggaaaatggg
     4441 agtataactt tgattgctag cactacagaa aatccatatc attatgtata taaagctctt
     4501 ttaagtaggt ctactgtatt tgaatttaaa cctttagaaa aacttgatat agaaaaagga
     4561 ctgaaaagag ctgttgaagt attaaatgaa gatagctata tggatattga gtgtaataat
     4621 gatgctattg aagatatagc aattctttct gatggagata tgagaagagc cttaaatatt
     4681 ttggaagtgg tagtctattc aactaaacca aataaggata atataactta tatagattca
     4741 gatgtaataa gggcatctac atttaataaa attataaact atgacaaaaa tggtgatagt
     4801 cattatgata ttttaagtgc atttcaaaaa tctataagag gaagtgaccc acaggcatct
     4861 atacactatc tagcaagact tataaaaggt ggggatttga taagtatatg taggagactt
     4921 ttagtaatag ctagtgaaga tataggttta gcttatccaa atgctattgt aattgtaaag
     4981 gcatgtgtag actcagctat gcaacttgga tttccagaag cgaaaatacc attagcagaa
     5041 gctactattc ttttggctac atctccaaag tctaattctg catgtatagc aataatgaaa
     5101 gctatggatg aactggataa agagtttgtt aaggatatac caaatagtat aaaggatgct
     5161 cactattctg gttctaaaga aataggtagg ggttgtggat acaaatatcc tcataatttt
     5221 aaaaatcatt atgtaaagca acagtattta ccagatagca taaaagatag tatttattac
     5281 ataccacaag aaaataaaac tgagaaaaat ataaaaaaat atttagaata tctaaatagt
     5341 gatttttaga ctgaacttta tgtatcgggg gtagatatta tgattataaa acttaactca
     5401 acttatcaca gtagagttat gaaatactta aaaaaagaac ctgagtacaa tttgtttata
     5461 ataggggata ttgaaagata tggatatgga aataattttt taaatatatg ggcagatgtg
     5521 ggtgaacatg gtgaaataaa ggcaatatta cttaaatatt ttgaatttat gatgttttat
     5581 tctgatggag agtatgatgt tgaaggtttt tataatctac ttcgtaatac taattacgaa
     5641 gaaatatctg ggaaaatatg tgctgtagat gctttagcaa agagattagg acttaataat
     5701 ttaaaggtag ttgatttttg caaacttcaa actaaaaagt ttttgataga taataattgt
     5761 aatgcaaagg ttaagagaat taggcttgga aatctaaaaa agacagtaaa actttatgac
     5821 ttaatagatg aatttcacag tacaacttta gagaatttaa aaaatggact aaggacaggt
     5881 agaggatatt gtatagagat taacaaacag gtggtgtcaa tggcaaagag tacttcagaa
     5941 aataggactc atgcaatgat aattggagta ggaacacatc ctaaatatag agctaaagga
     6001 cttgctacaa aatgcctaat taaactatgt tcagagttac tgagagaaaa caaaattcct
     6061 tgtttgtttt atgataatga agaggctgga aagatatata aaaaactagg atttgaaaac
     6121 attggaaaat ggggaattta ttcaaaataa aaaattaaaa ttaatcttga caaaattagt
     6181 agggtataat aaaattatct tgaaagtaat tcctactaaa acagtatgaa ttagatttct
     6241 atatagaggt gataatatat gaagttgtct actaaaggta gatatggact aaaagcaatg
     6301 tttgaattag cattaaatca agacaatgga ccagtatcat taaaatttat tgctaagaag
     6361 caaaaaatat cagatcagta cttagaacaa atattttcat ctttaaaaaa atcgggtcta
     6421 gtaaaaagtg tgagaggtgc tcaaggaggt tatttgcttt caaagaatgc tgaagacata
     6481 acagtaggag atatacttgt agtattagaa ggaccagttg cattatctga ctgtgtacta
     6541 gatgaagatg tatgtgaaaa ctcaaacatg tgtgtaacaa aaatagtttg ggaaaagatg
     6601 aaaaagggta tagaagatgt tatagattca attactctta aagatatgat aaatgactat
     6661 aacaaaaata aattagaaaa tgatataaca aatattaaaa aatagatttg gagatgaatg
     6721 taatggaaaa aagaagattg tatatggatt attctgcaac aacacctatt aaaaaagaag
     6781 tattggatgc aatgatgcca tacctaacag attattttgg taatgcatct agttttcaca
     6841 cattcggaag agaagcaaaa gatgctttgg ataaagcaag agaacaagta gcagccctta
     6901 taaatgctga acctagtgaa atatatttta cagcaggtgg ttcagaaagt gataactgga
     6961 cactagaggg tgtagcttat gctaataaaa acaaaggtaa tcatataata acttcaaaaa
     7021 tagagcacca tgcaatactt catacatgtg aatacttagc aaaacatcat ggatttgaaa
     7081 taacttattt agatgttgat agcgaaggaa aagtagactt aaagcaacta gaagattcaa
     7141 taaaagatac tactatactt ataagtataa tgtttgcaaa taatgaaata ggaactatac
     7201 agcctattaa agaaatatcc gaaatagcta aaaaacataa gatattattc cacactgatg
     7261 cagtacaagc tacaggtaat attccagtag atgttaaaga attaggaata gatttgatga
     7321 gtatgtcttc acataaaata tatggaccaa aaggtgtagg agctttatac ataagaaaag
     7381 gtgtaagact tcataacttt gtccatggtg gagcacaaga aaaaagcaaa agagctggta
     7441 cagaaaatat acctgcaata gttggatatg gaaaagcagc agaattagct aaggaaaata
     7501 tgcaaaatca tgtagaaact ttaactcgtc ttagaaataa gttaatagat ggtgtgttag
     7561 aaagaatacc ttacacaaga gtaaatggta gcttagaaaa tagattgcca ggaaatgcaa
     7621 actttgcatt ccaatttata gaaggtgaag gaatactttt attattagat atgttaggaa
     7681 ttgctggttc aagtggttct gcatgtactt ctggttcatt agacccttca catgtacttc
     7741 ttgcaatagg tttaccacat gaaatagcac atggttcatt aagacttaca gttggagatt
     7801 ttacaacaga tgatgatata gattatatac tagagaactt accaaaagta atagaaagac
     7861 ttagaagcat gtcaccactt tatgatgatg cgaagaaaca aggtttagta aaatagacat
     7921 aaagataaag cataaaaaat agacataaat tgagtaggag gaaaagataa tgcaatatag
     7981 tgataaagtt atggaacatt ttatgaatcc aagaaatatg ggagaaattg ataatgcaag
     8041 tggtgtaggt gaagttggaa accctacatg tggagatata atgaagatat ttttagacat
     8101 agatggagat gtaataaagg atgttaagtt taaaacattt ggctgtggtt cagctatagc
     8161 aagttcttct atggcaactg aaatgataaa aggaaaaact ataaaagatg cattagaact
     8221 tacaaataaa gctgttgcag aagctttgga tggtttacca ccagtaaaga tgcattgttc
     8281 agttttagct gaacaagctg taaaagcagc tcttatagat tatgctcaaa agaacaatat
     8341 ccatatacca gaattggatg gatatgttat agatgatgct catgaccatg atgttgaaga
     8401 agaagaataa aaataagtga ttgtgttatg agaagtttac aatgattaaa aagaagtact
     8461 aatgatataa ttaggacata aaaaatagct gtttttaaaa tagctatttt ttattgccta
     8521 aattaagtat tttaacttga tttagaggtt aattagttaa caaaataatc cataagattc
     8581 aaataataaa gatgattatt tttttagaaa atagaacaag ctatatttta aataagtaca
     8641 atacttgctc tatatggtat aataataagt aatgcaaaag atgaggtgaa atttgtttat
     8701 gaataaaaaa gtaatgatag gtatgagtgg aggagttgat agctctgtag ctgcatatct
     8761 tttaaaacaa caaggatatg atgtcattgg tgttactatg aagttgtggc aagatgatga
     8821 tgttgttgaa attgaaggtg gttgttgctc tttatctgct gttgaagatg ctagaagggt
     8881 agcaaataaa ataggtatac cattttatgt tttaaatttt agagaagtgt ttaaagaaaa
     8941 agtaatagat tattttatag atgaatattt ggaaggaaaa actcctaatc cctgtatagc
     9001 ttgtaataaa catataaaat ttgatgattt ttataagaag gctagacaaa taggttgtga
     9061 ttatgtagct acaggtcact atgcaaagat agaaaaggat gaaagtacag gaagatattt
     9121 gctaaaaaaa tctgttacag ataaaaaaga ccaaacttat gcactttata atcttacaca
     9181 agagcaacta gagcatactt tattgccaat aggtgactat gaaaaagata gagtaagaga
     9241 aattgctaag gaaatgggta tggcagtaca caataagcct gatagtcaag aaatttgttt
     9301 tgttaaagat aatgactatg caaattatgt aaaaaaacat tctaaaaagc gtattgaaga
     9361 aggttttttt gtagatacta aaggcaatat acttggaaaa cataagggta tattatatta
     9421 tactataggc caaagaaaag ggcttggaat tacttttgga aaaccaatgt ttgtaataga
     9481 cataaatcca ataaataata ccatagttct tggagacaat gaagatttat ttaaaaagga
     9541 acttattgct aaagatgtta actttatatc aatagatacg ttagaagaac ccttaagagt
     9601 tcaagccaaa ataagatatt cagcaaaacc atcaccagct actattcata gagtaggaga
     9661 agatactatt aaaatagttt ttgatgaagc tcaaagagct attactaaag gtcaatctgt
     9721 agtaatgtat gatggagata ttgtagtagg tggaggtata atcgagaaaa gtttataaaa
     9781 acatctaaaa aattaaaaaa ataagcaata aagtatatta agagtaaata aagtttataa
     9841 aataaattta ttgtatatga aaatatagta ttgattaatt ttgtaaaatt tagtaatata
     9901 attagtatat taattaaaaa acaaatacag tgataaggaa tagtaaaata tttgatgtct
     9961 tacagagaga gggaaatgct ggaagccctt agaatatgat atttgaagca gccttttagt
    10021 agcaatcttg aacaatttat cttttataaa gaagatttaa gtagagattg acggtagtta
    10081 cgttatagct aaggttttcc gaagatatca tactttgtat gataattagg gtggtaccgc
    10141 gattgtcctc gcccctataa atttatttat agggacgagg ttttttattt aaacgaaaaa
    10201 ataaaattta ttaggaggaa catgatatgg aaaagatggg attaaatgaa ataagaagta
    10261 agtttttaga attttttgag tcaaaaggtc attatgtagc aaatagttat tctttagtac
    10321 caaacaatga taagagtttg ttacttataa actcaggtat ggcacctttg aaaaactact
    10381 tttcaggtgt agaagtacct ccaagtgtga gaatgtgtac atctcaaaaa tgtataagaa
    10441 caggagatat agaaaatgta ggtataactg caagacatgc tacattcttt gaaatgatgg
    10501 gtaatttctc atttggagat tactttaaaa gagagtcaat aaaatgggga tgggaatttg
    10561 ttacagaatg gctaaatata ccagaagata agatatgggt gactgtatat gaagaagatg
    10621 atgattctta tgatatatgg gcaaaagaaa tgaatttccc agaagaaaga atggttagac
    10681 ttggaaaaga tgataatttc tgggaaatag gtacaggtcc ttgtggtcct tgttcagaaa
    10741 tatattttga tagaggagaa gaatatggat gtgacaatcc agactgtaaa ccaggctgtg
    10801 attgtgatag gtatttagaa ttttggaacc atgtatttac tcaatttgat agagatgaag
    10861 aaggaaatta tagtctatta gaaaacaaga atatagatac aggtatgggt cttgaaagaa
    10921 tgggatgtat tatgcaaggc gtagacacta tctttgaagt tgatacaatt aaatctatac
    10981 tagaagcagt tgagaagtta acaggagtaa aatatggaga gaatccaaaa aatgatatat
    11041 caataagaat aataactgac catataagag ctgtaacatt cttagttagt gatggagtgc
    11101 ttccatcaaa tgaaggaaga ggatatgttc ttagaagact ccttagacgt gctgctcgtc
    11161 atggtaagtt attaggagta aaagaattat ttttacagaa actaatagat gaagttataa
    11221 aagtaaatga taaagcatat ccagttttag ttgaaaaaga aagttatatt aaaaaagtag
    11281 ttggaataga agaagaaaaa ttcaatgaaa ctatagacca aggaacagaa atattgaact
    11341 cttatataga agtattaaaa aatgaaggaa agactgtcct aagtggtcaa gaagctttta
    11401 aactatatga tacttatggc ttccctatag acttaactaa agaaatatta gaagaagaac
    11461 atctatctgt agatgaagaa gcttttaatg aagaaatgga aaagcaaaaa gaaagagcta
    11521 gaaatgctag aggaaatatg gatggagaaa gttggaagga agaccctcta tcaaaattag
    11581 aatctacagt tgacagtaca tttaatgggt atagtgaaat ttatggtgaa ggaactattg
    11641 aagctatagt taaagatgat gagctagttc aaagtgcaga agaaggcgat aaggtatcta
    11701 tagtactaga taacactact ttctatcctg aaggtggagg acaagttgga gattgtggat
    11761 tgataactaa tgaaaactta gttttagaag tattaaatac taaaaaaggt gctaataata
    11821 gtataaaaca tataggtata ataaaatcag gaagaataag taatggggac aaagtgaaga
    11881 cactcgtaga tagagaaact agaatgtcag ctgcaagaaa tcacagtgct acacatttac
    11941 ttcataaggc attaagagaa gtattaggag aacatgttaa tcaagctggt tctttagtaa
    12001 ctccagaaag acttagattt gatattactc actttgaagc gatatcaaat gaagaattaa
    12061 aagtaataga agaaaaagta aataatgtta ttttatcatc tttagacata aaatgtgata
    12121 ttatgaatat aaaggaagct aaagaaaaag gagctacagc actatttggt gaaaaatatg
    12181 gtgatgaggt aagagtagtt tctatgggag attattctac agaactttgt ggaggaactc
    12241 acttgacaaa tacttctcaa gtaggaatgt tcaaaatatt atctgaaggt ggagtagcag
    12301 caggtgtaag aagaatagaa gcaataacag gaaaagctgt ttatgaatac ctaaaagaaa
    12361 gagatggaat tatctctgaa gtttgtgtaa acttaaaatc taaagaagat aatttaattc
    12421 aaagaataag ttctttatta gaagaaaata aaaacttatc taaagaattg catgatatga
    12481 aagctaagat gagtttacaa tcagttgatt ctatttttga ttctaaagtt gaagtaaatg
    12541 gagttaattt gataactaat aaatttgaag gtatggatat ggatacacta agagaaacag
    12601 cagataatct aagagataag cttggttctg gtgtagtagt acttgcaaat gtagtagatg
    12661 ataaagttaa ctttgtagtt actgccacta aagatgtatt agataaggga atacattcag
    12721 gtaatattgt tagagaagtt gcaaaaatag ctggaggaaa aggtggagga agacctaata
    12781 tggcacaagc aggagctagt gatgtatcta aggttgacca agctttaagc tatgcaagtg
    12841 aggttattaa gacacaagtt aaataacatt cactcaagga ggtttttagt ttggaaaaag
    12901 atttagacta cactatgaaa tttgaaggaa taccagaaga taggatgagt gtaggagata
    12961 ctattgattt tgtatataaa gcattagttg aaaaaggata taaccctata aatcaaatta
    13021 tagggtatct cctttctggt gattctagct atataactag tcataaaaat gcaagagcta
    13081 taattaaaaa atttgaaaga gatgagatac ttgaagaagt aatcacgcac tatctgaata
    13141 gaaagtaggt caaataaatg agaaaaaaga ttatgtcgtt gtttgcaata gtcattatga
    13201 tttttgtatc aactgctagc tatggttttg ctgaagaaaa aggcaaggtc atatttattg
    13261 atatgaatag gaccaatctt ggaagtatga tgaatattcc aatcttgaaa aatgagatag
    13321 aaaagagagg ctatgtagct ctcatgaata taagagggga tcaaggtact gatgatagaa
    13381 ggtcttatgc aagtatgggt gcaggaacaa gagctagtgt aactactgaa gactatatca
    13441 attttcaaaa agtagaccaa gatactgcta aagcttttga agtcgcaaca ggtcaaaaag
    13501 ctaaagaaat aaatgattta ggcattaata gatctataaa tgaaaatctt gagtatggag
    13561 aatatggagc aaatttaggg tcattaggaa aaactttatc agaaaatggt ttaaagacat
    13621 ctgtaattgg aaatgctgat ataattgaaa ataatcaatt agttaaaaac agaaacttat
    13681 gtttagtagc tatggaccaa tatggaagga ttgattcagg taatattgat aatattaata
    13741 agaaagattc taaaatgcca tttggaatat ctacagatta tgataaatta ctagatgaaa
    13801 ctaaaaaatc ctataaaaat agtgatgcta tatttgtaga acttggagat acatatagat
    13861 tagatatgta tagattaaat ctaaatgata atacttatga tataatgaag aaaaatattg
    13921 taaaaaatat aaataaatat cttgaacaag tattttctat ggttggagaa aatgacactg
    13981 tttatatagc aagtgcattt ccaagtgacc tagattataa gaataagaga agactttctc
    14041 caataataaa atttagtgga gaagggaaag gtctactgga atctgccaca acaagaaagg
    14101 ctggaattgt tgccaattta gatgttggag tagatatact aggtacattt ggtcttgaaa
    14161 ataaagagat ggtaggtaga agttatactt tagtaaataa agaggacaat attagctatc
    14221 taaaatcact ttatgaaagg atagtgtcta tatcttcaat aaggtcaact attataaatg
    14281 gctttgtagg cgtaatatct gcttcttggg taattgccat gtttgcgatt ttctttagaa
    14341 attatatgcc acataaagag cgtgtattca aagtacttaa ggagtttata aaattaggca
    14401 taataatgcc actatcattt atgttagctc ctttgatgaa ttttaaaact ccaatttcaa
    14461 tatctttggg aatatttgca actacgctaa tattatactt gttaggtaga aaattattta
    14521 aggacaatga tataaagcac atgggatttt ttgcaacact tactatttta gtcatagtta
    14581 tagattctgt atttggaaca tacttaatgc aaaataacat aatgagttat gatgctgtca
    14641 taggagctag gtactatggt gtaggaaatg aatatgaagg tgtaactata gcaagtgcag
    14701 tatttggctt agcagtatta ttacattata agaaggtatc taaattactt gttgtaattt
    14761 tttcattaat aatacttata actagtgcat atccttcaat gggtgcaaat gttggtggag
    14821 ctatatcaga atgtgtagca tatttgttgt ttataatgct tatatatgac attaaaatag
    14881 attttaagaa agctgtatta ttagctgttt cagctgtagt tgtcgttttt gcatttgcag
    14941 ctttagatat actttcaggt agtgaatctc atttaggtat gtttgtacag agaattttcc
    15001 taaatggacc aggagagata ttacaaacat ttggaagaaa aattcaaatg aatatgcaac
    15061 tagcacagac aagtgtatgg gtaaatattt tacttgtagg aattggagta attgcagtgc
    15121 ttatatttaa accatctaga cactttagaa agataatgaa taattttcca atcttgttta
    15181 aagggtttat tgcatctatg gttggttgta taattacact tttagttaat gattctggaa
    15241 tagttgctgc atctacagca tctatatata tacttatacc actaataata ataagtataa
    15301 atatgataat atttgaaaaa aataactaaa aaaattttta ctgttcatat aggatttaaa
    15361 taaaaaatgc aatatgatta ttaatgtaaa ctggatgctt acataaaaaa tcatattgca
    15421 atttttatga ttgattatgt aaaattatat gaagcagtaa gcctgtgacc ttagttatga
    15481 gaggttcaga atatgtattt aatctataga atataaatag ataaaggagg aactatgtta
    15541 gatggaagaa taatgggtct tgatgttggt gataaaacta ttggagttgc tgtgagtgat
    15601 ttgatgggat taactgcaca aggtgtaaaa acaataaaaa gagttggcaa gaaaaaagat
    15661 attgaagagc taaaagcaat aataaaagaa aaacaagtaa ataaaattgt atcaggatta
    15721 ccaaaaaata tgaatggaac tttaggtcca caaggagaaa aagtaatcaa gttttgtgaa
    15781 ttggtaaaag cagaaacagg tattgatgta gagttttggg atgagaggct ttctacagta
    15841 gcggcagaga ggtctttatt agaagcagat gttagtagac aaaaaagaaa aaaggttata
    15901 gatatgttgg cagcagtaat aattttgcaa ggatatttag attttaaaat aaattcataa
    15961 aagaactata aaattgaata cttatgatat ataatataaa taataaaaat aaaaataaat
    16021 ttgaggtgaa tgacattatg gaagaaaata taataaattt aatagacgaa aatggcgttg
    16081 aaagccaatt tgaaataata ttaacattag aagcagaagg aaaagagtat gcaatattaa
    16141 tgcctttaga tgatgaagaa gctgaagaag ctttaatctt tagaattgat gaggatgaag
    16201 aaggtgaaat cttaatacct ttggaaagtg atgaagagta tgaaactgtt gtagctgtgt
    16261 atactgctat aatggaagaa gaaggtttaa attacgacga agatgaatca aatggactta
    16321 actagataag taattaagtt tgtatataaa aatcagatat ttattatatt tgctttaatt
    16381 aaacattatt aattgtaaaa gcatttatag tatatatctg tttttttgtt ttttattaaa
    16441 aataccaaaa aatttatgaa ttttggtgaa taatatatct tttatgggta tatatttata
    16501 ttatgagaat aaattaaatt tatttaaatg tcttgacaaa aaatgaatta tcaattacaa
    16561 tattatttaa taaagattat caattgaaaa tagtaagggt gatgttatgg caaatacaat
    16621 ggatttatta aaagacaagt taaaagaaac tggttttaag attacgccac aaagaagagc
    16681 aatagttgaa atattgctta aacatgacca ttcacatcta agtagtgagg aaatctatga
    16741 tttagttagg gttgactgtc ctgaaatagg cttagctact gtatatagaa caatgcagct
    16801 attggatgaa ataggattaa tatcaaaact aaacttagac gatggatgta taagatatga
    16861 gattagtcta cataaagaag actgtcataa tcatcatcat cttatttgta aaaattgtgg
    16921 aaagataatg gaagctaaag aagacctttt agacaatata gaaaaagaga tacaatcatt
    16981 gtacaaattt aaaatacttg atcatgatgt taaattttat ggactatgtg acgagtgtaa
    17041 tggagtaagc gattcagaag aataaacttc tattgcttaa tgtcgatgga agaatagtat
    17101 agaactttta tattctatgc tattcttcca ttttttatta taaaccatct ataaaatgca
    17161 aatatacagt tttcaagatg cttatagttt taatattgaa aatataatta atcctataag
    17221 tacaaaaatg aatgattttt ctatgaaatt actaaatggt cctccaacag ttaaaaaacc
    17281 taaagaaatt ttcttttcgc ttagcggata aaaaattggt attccttgct ttgtaaataa
    17341 atctcctaaa aacatatgac ttgcataacc aatcgtacca taaaaagata aattattaat
    17401 attagtgatt aattctattt gttttagtaa aaaatatact attatagttg caaatatgct
    17461 atgagaaaaa cttctgtgct ttaaccaggg aaaaattgac aacattagac aaaaaattgt
    17521 aaaatatatt tcaacttcta tgatgtataa gcatatagct aatagtataa atattaatga
    17581 tagaaaaact tttcttagaa atgtgtgggt tagttttgcc tcaataatta taattgcaac
    17641 aaatgtaatt attgcgctta aataaaaatt atcatttata tttattgaaa taaagaaaat
    17701 aataatatta acagcatata taaaaattct atatatgtat ttggatacat tgttttttag
    17761 aatgaaattt gagaccatag aattagattt atccaaatcg ggtagtacag caaaagtagc
    17821 tgaaactaaa atatcaacta aagatatagg aattttaaat attaaagatg tttggatagc
    17881 tgttaagatt cctattgcac aatgagtttt accacgcata aatcctccta aattatccga
    17941 tattaaatca tataatattt agaggatatc aaaataaaat aggtttgtca aaataatacg
    18001 ttaaatgtat tttaaatata gcttaaaata tgatacgaat tatggtacaa taagaataac
    18061 tggtagcgag tgcgtgattt gatattattt atgattgtag aacgtgttaa aaaattaaat
    18121 agacagaagg agatgatgca attgttcaaa aagaacacca acaagataaa ggtaatggcc
    18181 ttgggtggac ttaacgaagt tggaaaaaat atgactgttg ttgagtataa agatgaaata
    18241 attgttatag atgctggact tagttttcca gaggatgaaa tgttaggagt agatatagtt
    18301 ataccagata taacttattt agtaaaaaat agggataaaa taaagggtat atttattaca
    18361 catggacatg aagaccatat aggagctctt ccatatatat taaagaaaat aaatgtacct
    18421 gtatatgggg caagacttag tataggtctt atacaagtaa aacttaaaga acataaaatg
    18481 aataatgtta agttaaatgt tataggtcct agacaagtta taaagcttga taatatggaa
    18541 gtagagtttt tgaaaaataa ccatagtata ccagatgctt actctattgc aatacacaca
    18601 gaccaaggta taatatacca cacaggagat tttaagattg acttgactcc tattgatgga
    18661 gatgttatgg atatgcatag aatctgtgag ttgagcaaaa aaggagttct tttaatgctg
    18721 gcagatagta ctaatgcaga aaaaccagga tttactatgt ctgaaaagac tgttggggta
    18781 ggtcttgacg aattatttgc aaaaggaaat ggcagaagaa ttatagttgc tacctttgca
    18841 tccaacatac acagattaca acaaataata aatactgctg aaaagtttaa tagaaaagtt
    18901 gctatatcag gtcgctcaat ggtcaatgta gttggagttg caaaagaatt aggataccta
    18961 gatatttctg atgacatgct tatagattta aatgatatat gtaagtatga agatagtgaa
    19021 ttagtgataa taacaactgg ttcacaaggt gaacctatgt cagctcttgc tagaatggct
    19081 ttttcagagc acaaaaaagt agaaataaag agtggcgatt tagttataat atctgctcat
    19141 ccaataccag gaaatgaaaa attaatatca agagtaatca atttcttatt tgaaaaagga
    19201 gcagaagtag tatacagtga tatagctgat atacacgttt ctggacatgc ctgtcaagaa
    19261 gaattaaaac ttatacatgc attagttaga ccaaagtttt ttatgccagc tcatggtgaa
    19321 tatagaatgt taaaaagaca tgctgaaata gctgaacagc ttggtatgga taaagaaaat
    19381 atttttgtca tgcaaacagg agatgtttta gaattagata aaaattctgc taaggtagca
    19441 aaccgtatac aaacaggaaa catattagtt gatggtctag gagtaggcga tgtaggtaat
    19501 atagttttaa gggatagaaa gcatttatca gaagatggtc ttatgatagt tgtagttact
    19561 atttccaagg atgagggtaa ggtcttagca ggtccagata taatttccag aggattcgta
    19621 tatgtaagag agtcagaaga tttaatggat ggagcaaagg atataattaa gaatgttcta
    19681 aatgaatgtg aagagaaaaa tattaaagaa tgggcttatt taaagaataa tattaaagaa
    19741 aacctaaaag aatatttata tcaaaaaact aaacgaaatc caatgatact tcctatcata
    19801 atggaagtat aatataaaaa aactataaat ttgaaaatta gacctcttgt aatttttaaa
    19861 gtaatatcaa aaatccaatt gatattatct taagaattac aagaggtctt tttaatactc
    19921 attttaaaaa aatatatatt acataaaata ccaattataa caatctttta ttagtaaaaa
    19981 ctacttatat ataaatttta ctgataggag gattcttatg gatgatgttt caagacaaaa
    20041 tgctataaaa gctttaaaac aaacaaaaat ggaaatagca ggagagtatg gaatgaatta
    20101 tgaagatgct tttgaaataa tagaaaatgc gtctaataaa ggtgttttag aaggttactt
    20161 taaaaagctt gaaaagaaaa aaaatttagg tcaaggtata tctaggcatt tagaataaaa
    20221 aaagttaaat gtgagaaaat aagtttaaat aaactttaag aggtgacatc gatgaaaaaa
    20281 ttagctagta ctgctttggc tatattaatt gcgcttactc ctttaagctt ttcttttgca
    20341 aataataagg aaaatgctga tgccaaccaa ctaaatatat cttcaaaatc agcaattttg
    20401 atggatgttg gtagtggaca aattttatat gaaaaggatg ctcataaaaa gcttcctcct
    20461 gctagtgtaa ctaaggtaat gactatgcta cttatagtag aagctttgga ttcaggcaag
    20521 attaagcttg atgatgaagt tcaagtaagt gaaactgcat ctagtatggg tggtagtcaa
    20581 atattcctag agccaggaga aactcaaaag gttgatacat tattaaaagg tatagctgtt
    20641 gcttctgcaa atgatgcttg tgttgctatg gctgaacact tagctggaag tgttgaagga
    20701 tttgttgata gaatgaatgc taaagcaaaa gagttaaata tgaatgatac tcattttgca
    20761 aatacaaatg gacttccagt tgcaaaccac tatacatctg ctcatgatat agctttaatg
    20821 tctagagaac tattaaaaca cgaaatgata agtaaatact taactacatg gatggataag
    20881 gttgtagtag ggaagaaaca agttacagta ggtcttgcaa atactaataa acttataaag
    20941 cattatcaag gagcaactgg tgttaaaact ggttttactc aagaagcaaa atactgttta
    21001 tcagcatctg caaagagagg taatactcat ctagttgctg caactttagg agcagagact
    21061 tctccagaaa gatttaatga tgcctctagc cttttaaatt atggatttgc taattatgaa
    21121 agtgttaagt tatgtagtaa aggtgataat atagcaacac ttacattgga taaggctgat
    21181 gaaaacaaag ttaaattggt tgctaaagaa gatttaaatg ctcttattaa aaaaggaagt
    21241 tctaaagaat ttgagaagaa aattgaaata gtaaaaaatc ctaagatgcc aattaaaaag
    21301 ggaactgttt taggaaaaat aaagatatgt aaagataaaa aagtaatagg cgaagtggaa
    21361 ttgataaata ctaaggatat aaataaggca agctatctac aaatgcttca aagaataatt
    21421 gataatatga tataaataat tatcagatga aatataatta taatacacaa agatatgatt
    21481 ataatacaca taaaaaatac ccttatgtaa ttatttgcca taggggtatt tttatttacc
    21541 atatagaagt attttataat cttatgattt ttatagaaat atagaaaagt ttatgtatct
    21601 taaaatatca tttgatttaa tatcatatat agggttgttt aacaattatc taagtaaagg
    21661 catactacca gtaagtttat tcactttctt tttattccca taaattgcta tcccaaaata
    21721 ctgtaactca tcttctggaa cactagcaat cttttgtgta aattcttcat aaagattgca
    21781 actttgtgct acatctgaaa aatctacaac agttaaatcg ttaaattcca aagtatataa
    21841 ttttttacgt aaatctttta ttatttcttt gtttcctttt aaaataggta caggaatgtc
    21901 aataattcct aagtggctat tacctgtttt gtcaattaca tctgggccaa cagtttctgg
    21961 ggcatgtttt cctagtgtaa ttcccattat agctgctgta ttggatataa ttcccattgg
    22021 taaattttcg tctattacca tgacacattt tgaattttta gtttccattt ttatttctcc
    22081 tttttacctt atttaaagtt gtttgaatca tcatttataa atatattttg atactgcttt
    22141 ggtgtaagac caataaaatt cttaaaaaag tttgtaaagt gactttggtc tacaaatcct
    22201 gtttgtatcg cagcatctat aggttctacg cctttttcta aaagcttttt agctttgttt
    22261 acccgtatat tttctaggta tctatatggt gtaatacctt ttaatttcgt aaaattacga
    22321 agtagagagt acttattcat acctgctata gtgcttaatt cgtcaagtac tatatgttca
    22381 gcataattat tttctagata atcacatact gcttgaattt ctatatttgt attttccaag
    22441 ttagattgca aatttggctc tgtatgttct tcaattaatt gttcaattag gaagaaaaaa
    22501 agctcttcct ttttaaaatc caattcttct tccatgatta tatagtgtaa ttcttgtagt
    22561 agaggtacta gctcactgcg aaatataaca ggttgattaa attttggtaa ataattttta
    22621 cctgtaattt caaagacagt ttttttcatt atttctggct taatattaat acatctgtaa
    22681 tcaagtactt tatcatctat ttgttcacaa gtatgactat caaaagggtt aaaaagtagc
    22741 aaatctccag tacttgtggt atactcttta tttttacaag ataaatatct ttgacctttt
    22801 tctataaaac caattacata atgttcatga aaatggtttg ggaatttttg cattatgcct
    22861 ttaaatctat atgcttcaat ttttaattcc ctatcaaagc atactgtgcg ttcttcttgt
    22921 ctcacttagg taacctcctt tcatgttata caaagtgtaa cacatatttt tttatacttc
    22981 ttgtataata ttgactaaga gacaataatg tcaattcatg tcaatataat attacactta
    23041 cagtaggtgt aattattata ttttcacaat gaatatgata taataataaa agttataaaa
    23101 taaaaagcaa attttaattt tatgcctaga agttaaaatt attataatgt aaaataattt
    23161 tgattataag ctatttaatt gttataataa tattaaaaat ttaatttagg agatgtacat
    23221 taatgttaag tatgaatata ggagaaatag tagcaagttt agttggtata gctatggcaa
    23281 tatcaataca tgagtttggc catgcttatt cagctcactt attgggagat gatacagcta
    23341 aagcttatgg tagaatgaca ttaaatcctg taagacatgt tgacccaata ggtcttttgg
    23401 ctatgtttat ccttaagata gggtgggcaa agccagtacc agtaaatcca aataacttta
    23461 aaaactataa gataggaaac ctgatagttt cacttgcagg cgttttttgt aatgttttga
    23521 ctgctatact gtgtgtaatt atcaataagt atgtgcatat gtatgctata aatacaatag
    23581 ctgaatatgt gtttttatat agtattggat ttgcagcttt taacttactt ccgatacctc
    23641 cacttgatgg atggggagtt atatctacat ttgtaccata taaatggaat gaatatttgt
    23701 ataaatttga aagcatgagt tatataattt tattaattgc tttatttact ggtatgtata
    23761 gaataatatt aacaccaata tatgcagtgt tgctaaaaat agtatattta tttgtttaat
    23821 ataataaaca gattaaagag ggattgatat gaagtataat atacaattac aagtctatga
    23881 gggaccatta gatttacttt atgatttaat aacaaagcat aaaatagata taaaggatat
    23941 atccataata gatataacta agcagtatct aaattatttg aaaatgctag ataaaatgga
    24001 tttagagata actagtgaat ttataactat ggcatcaaaa cttttagaga taaagtctaa
    24061 atatctgctt tataagcaaa aagatgaaga agaagaccca agaatagaac ttatggaaaa
    24121 gttagaagaa tatagaaagt ttaaggtagc atcacaagat ataaaagaaa atataactta
    24181 tgttaatgag agattttata gaaataaaga agagataatt attgatgaca atgtggattt
    24241 agaagatata tcaatagaag caataaaaaa tatacttcca tacatattta aagttaaaac
    24301 atcacagata gaaaatgcta atgatgagaa actagataaa atagtaagga agaaaataat
    24361 atctgtagaa gaaaaaatat tatatataag agatattata aaagagaaga ttgaagttac
    24421 ttttacaaat attataaaat catatgaaaa tgatgaaata atagctactt ttttatctat
    24481 attagagctt ataaaagaaa aagaaatagt cgtagtacaa gatatatttt ttgatgatat
    24541 tctaataaga aaaagttcgg agtgttaaaa tgaaaagaga agatataaag tatattatag
    24601 aatctgttat gtttgcatat ggtgaaccaa taagtataaa agaattaaac tatataataa
    24661 ataaagaact ttcatcaaaa gaaatagaga ttatgttaaa tttacttatt gaggagtaca
    24721 gagaacaaaa tagaggtatt caaataatca agttagagaa taagtaccaa atgtgtacaa
    24781 ataaagacta tgcagaatat ataaagaaga ttattgaacc aaagaaaaag aaaagtttga
    24841 gccaagctac attagaaact cttactataa ttgcctacaa acaaccaata acaaaggtag
    24901 aaatagagga tataagagga gtaaaatgtg ataaggtatt acaaactttg tttgaaaatg
    24961 aacttataag agaagctggt agattgaata agatagggaa accaataatt tataaaacaa
    25021 cagatgaatt tttaaagttg ttgaatatag aaagtcttga agaattgcca cctatagaaa
    25081 attatcaaga agtggcaaca aatgaataaa aatataaaat cacaataatt tagtatgtaa
    25141 ttttaaattt taaaagattt aaaatgctct aagttgttgt gtttttttgt ataaatataa
    25201 aattataatt ctcttaaaat aaatatggat aattaagtat aaatagtaat tttttgatga
    25261 taatataaac atggagctta atgaattatt taattttaag tatttaataa tagctgtaat
    25321 ttttattata agtgtaacta tgttgatttt aatatctcat ataaacatct taataatagc
    25381 tgatataaaa aacaaagata tttgtttaaa gcttaatata aaatacatgt ttaatttaat
    25441 aaatataaat agacaacttt atccagctga aaatagtaaa aataatgata aaaaagaggg
    25501 gatgaagaat aatatagatt caagtatatt attagctgat gaccttttga gcatttatag
    25561 attattaaaa aagataaaaa tacatgaact ttattcaaat attaattttg gaactggaaa
    25621 tattggctta acatcttctg tatatgtatt gataaatact ttgtatggaa atttatttaa
    25681 tatgattgat gcagaaaaaa tgtatttaaa tgtaaatcca gatttcacaa aagattatgt
    25741 tttaggaaat ataaggatac atataaggcc aagaataaaa gctttattta atattataat
    25801 aatgattaat aaaattatga ataaaaataa aggaaataag gaaggtgata gtaatgagag
    25861 caacaggttc gatacagagt cttatggaaa caactcttga aacaattaaa ggttctatag
    25921 atgcaaatac tattatagga gaccctataa aaacagatac tacagtggtt gtacctatat
    25981 caaaggttac aattggcttt ggtataggag gaggagaata ctcaaaagga tatgatgata
    26041 aagataggga aattgaatta aaaaatgaaa aatcagatac gaattttgct ggaggtagcg
    26101 caggggctat atctgtacaa ccagtagcat ttgtagtagt tgaaagtgga gagactagaa
    26161 taatgagttt agatagtaat atcaatctag tggataatat attatctata actcctagag
    26221 tattggagaa aattcaaaat atatcacaaa gtaataagaa tcaagataaa aacaatatgt
    26281 aagaataaaa tatttttaaa tatgtatata gttttaagta aagtagtggg gttaatataa
    26341 atcccactat tttgttgtgt actttaaaca tttatatcta ataagatttt agtttaaaac
    26401 aatttaaata gataataaag ttgatatata gtataattat aaaaagaaaa gaagtattat
    26461 attatagata tataaacgca tttgagatat aagagggagt tataaatcat tagttttgag
    26521 gtgaatgtta tgagcgataa agaattatct attttgttgt tagaaagtat gaataatttt
    26581 cataatttaa taaagattat taataacgaa agatataaaa aagataaggt tcttacagaa
    26641 agacagttct ttgccttagt aaaaataaga aaacatgata aaatagagct taaaaatcta
    26701 agtagagatt tgcatgtatc tacttctagt ctgtgtatac tacttaataa attggttgag
    26761 caagagtatg tatataggga agaagatagt agagatagac gaaatacttt ttatggaata
    26821 actaaaaatg gagaaaagat attggataat gaaatactaa agtttgtatc tataataagt
    26881 gataaaatgg attgcctaga tataaataat aaagataaat tatttacttc tttggaagag
    26941 tctaaaaata taatagaaca attattttga aaaggagaat ttattatgaa tataaagtat
    27001 tctaaagaaa tattaaaaag tttagaaaat gctataacag tagtagctct atttgaggga
    27061 gaagatgttt ctacgacatt tgataaaata aatatgataa gcagaaattt taattctaaa
    27121 gaaaatagta tagatactgt aacattggtt gaaaataaca atttggtaaa tgtttgtctt
    27181 gttggttttg gtaagagaga gttattgaat agagaaaaaa ttagaatcat aggtgggaat
    27241 ttgtgtaaaa aattaaaaaa tatattgaaa aatgaagcat ataagagtag tgatgttaat
    27301 gtactgaatc ttaatttaaa tagtgatgaa ataggttgtt ttgtagaagg gatattactt
    27361 ggaaattata agtttgataa atataagaca aaaagtaagg attttgaaag aaaagaagtt
    27421 gatactataa ctatatttac tgaaaaagaa gttgagtgtc aaatagaaaa ggctaaaata
    27481 ttagctaatt caactataat agctagaaat ctagttaatg aaccatcaaa cgtaatatat
    27541 ccaaaaacat tagcagtaga aacagttaaa cttggtgcag agtttgggtt taatgttgat
    27601 gtttatgaag aagaaaaaat aaaggcattg ggaatggatg cttttttcgc tgtttcaaga
    27661 ggttcaatta ataaaccaag attcatagta atgagatact ttggagataa agaaagtaca
    27721 gatatattag gtctagtagg taaaggattg acttatgata ctggtggata ttctttaaaa
    27781 tcaaatgctt ctatgctaga tatgaagaca gatatggctg gtgctgcgag tgttataggt
    27841 gctatgtgtg ctatatctca aagtaaatta aagaaaaatg ttatagcagt agtggcagca
    27901 tgtgagaatg ctttatcagg aggttcatac aaacctggag atataattag ttctatggca
    27961 aagaaaacta ttgaagtttt aaatacggat gcagaaggta gacttacatt agctgatgca
    28021 atatactaca ttataaacaa tgaaaaagta acaaaggtgg ttgatgtagc tacattaact
    28081 ggtgctgctt taatattgtt aggtaatgtc gcaactccta tagtaacaaa taatgatgat
    28141 ttttattgtg aattagaaaa agctgccact ttatcagggg aaagggtatg gaagatgcca
    28201 atttatgatg aatttaaaga tatgataaaa ggagaagaag cagaccttaa aaacactggt
    28261 ggtaaaaatg ctggatgtat aacagcagga gcttttattg gtgaatttgt tgggaatact
    28321 ccttggatac atatggatat agcggggaca agtacatcat caaaatcaat tggatacaaa
    28381 gcaaaggggg ctacaggaga acctgttaga acattgtact atttggcaga aatctaaaaa
    28441 acaattataa aatataattt ataaagccac gtggatattt ttgtaattta agattatcta
    28501 tgtggcttta ttttttattt aaataaatta tttactcttt ttcttttttt caagtataaa
    28561 agtatctcta ttaaataaaa gagcaggtat gtacataata ctagtaacaa aaataaacgt
    28621 aaaaatagca gttgtgctat gacttaaaaa accataatag tacctagagt tggtaaatgt
    28681 gtttagtaaa aatatgaatg aaaaataaat aaaacatata gctaagttat ctctaagtgc
    28741 atatctgtta actctaaatt ttctaacatc aaccatatat atatctgcat aaattatcaa
    28801 gcacttagag aagataaaga taaaatatat taagctagga aattcatagc ccaagttttt
    28861 agaaaaaatg agttctccaa ctgaacaaac taaactccaa gaaaaaaata tattaaaaag
    28921 atgatactgc ttaaaaatta gtatggctat acacagatat atacaaatat aattgattcc
    28981 aattggtaaa gagtttagag tactataatt acctgaagaa ataagtaata attgctctag
    29041 agccataatt attacaagta atgtacaaat tatcttctca actgtatagc tataaggtaa
    29101 aatatttttg gttagttttg gacaatagta taaaaataat ccaaatataa agagacttac
    29161 aaattgttca ctagaaaaaa gaaaaatttt aaacatataa cattctctcc tataataaat
    29221 ttaatatgta tctatat
//
LOCUS       DAYATN010000037        29184 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.037, whole genome shotgun sequence.
ACCESSION   DAYATN010000037 DAYATN010000000
VERSION     DAYATN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29184
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.037"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            227..2869
                     /gene="adhE"
                     /locus_tag="ACGI8A_001598"
                     /gene_synonym="adhC"
     CDS             227..2869
                     /gene="adhE"
                     /locus_tag="ACGI8A_001598"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFH0179599.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3229..3570)
                     /locus_tag="ACGI8A_001599"
     CDS             complement(3229..3570)
                     /locus_tag="ACGI8A_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179600.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3597..4268)
                     /locus_tag="ACGI8A_001600"
     CDS             complement(3597..4268)
                     /locus_tag="ACGI8A_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179601.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4280..6856)
                     /locus_tag="ACGI8A_001601"
     CDS             complement(4280..6856)
                     /locus_tag="ACGI8A_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0179602.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            7064..7999
                     /locus_tag="ACGI8A_001602"
     CDS             7064..7999
                     /locus_tag="ACGI8A_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFH0179603.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8018..8755
                     /locus_tag="ACGI8A_001603"
     CDS             8018..8755
                     /locus_tag="ACGI8A_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFH0179604.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(8940..13997)
                     /locus_tag="ACGI8A_001604"
     CDS             complement(8940..13997)
                     /locus_tag="ACGI8A_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFH0179605.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(14022..14441)
                     /locus_tag="ACGI8A_001605"
     CDS             complement(14022..14441)
                     /locus_tag="ACGI8A_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179606.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14675..14926)
                     /locus_tag="ACGI8A_001606"
     CDS             complement(14675..14926)
                     /locus_tag="ACGI8A_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179607.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            15015..15746
                     /locus_tag="ACGI8A_001607"
     CDS             15015..15746
                     /locus_tag="ACGI8A_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFH0179608.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            15805..16716
                     /locus_tag="ACGI8A_001608"
     CDS             15805..16716
                     /locus_tag="ACGI8A_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0179609.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            16767..17579
                     /locus_tag="ACGI8A_001609"
     CDS             16767..17579
                     /locus_tag="ACGI8A_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFH0179610.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            17688..18380
                     /locus_tag="ACGI8A_001610"
     CDS             17688..18380
                     /locus_tag="ACGI8A_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0179611.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18611..19408
                     /locus_tag="ACGI8A_001611"
     CDS             18611..19408
                     /locus_tag="ACGI8A_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179612.1"
                     /translation="MSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKK
                     NEEISNNKNLNNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENN
                     KYTSKEKNSEDIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSI
                     KKDVTNARNKCKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALK
                     LINTKSPKYIGKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19445..20602
                     /locus_tag="ACGI8A_001612"
     CDS             19445..20602
                     /locus_tag="ACGI8A_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFH0179613.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            20754..21314
                     /locus_tag="ACGI8A_001613"
     CDS             20754..21314
                     /locus_tag="ACGI8A_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFH0179614.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21342..21833
                     /locus_tag="ACGI8A_001614"
     CDS             21342..21833
                     /locus_tag="ACGI8A_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179615.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            22062..22613
                     /locus_tag="ACGI8A_001615"
     CDS             22062..22613
                     /locus_tag="ACGI8A_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0179616.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            22699..23631
                     /locus_tag="ACGI8A_001616"
     CDS             22699..23631
                     /locus_tag="ACGI8A_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFH0179617.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            23686..24549
                     /locus_tag="ACGI8A_001617"
     CDS             23686..24549
                     /locus_tag="ACGI8A_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFH0179618.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            24796..26658
                     /locus_tag="ACGI8A_001618"
     CDS             24796..26658
                     /locus_tag="ACGI8A_001618"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFH0179619.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26661..28127
                     /locus_tag="ACGI8A_001619"
     CDS             26661..28127
                     /locus_tag="ACGI8A_001619"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFH0179620.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            28151..28987
                     /locus_tag="ACGI8A_001620"
     CDS             28151..28987
                     /locus_tag="ACGI8A_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFH0179621.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT        10804 a         3496 c         4664 g        10220 t
ORIGIN      
        1 gataaaaaat tttagtaaaa gtaaaaaaaa taatatattt gttaaaaaat aaattgacaa
       61 aatattcaga aaaaaacttc ttgacctaag agagtaaaat aaataaaatc taaggtatag
      121 agatgaaatc tataaaatta ttttaagtat aagttaaaaa tatagatgat gcatttaaga
      181 tttataaaaa ttagagatgc ataattattt tagggaggtt tttagtatgg agaaaaaaga
      241 aaaggtagta gaaaaatcaa atgttgaagt atgtagtcct gaaattgtca acagtgttga
      301 aacattaaga atgagattag aagaaattag attggcacaa aaagaatttg caacatttac
      361 gcaagaacag gtagataaga ttttcttagc tgcttcaact gcagctaatc aacaaagaat
      421 acctctagca aaaatggcag tggaagagac tggtatgggt attgttgaag ataaggttat
      481 aaaaaaccat tttgcctcag aatatatcta taatgcttat aaagatacta aaacttgtgg
      541 tgtcatagaa aaagatgaag catttggttt tacaagaatt gctgagccag taggtgtttt
      601 atcagcagtt ataccaacaa ctaatcctac atcaactgca atattcaaat ccctaatagc
      661 actaaaaact agaaatggta taattttctc tcctcatcca agagctaaaa actgtactat
      721 agaagcagct agagttgtac atgatgcagc agttaaggca ggagcaccaa aaggacttat
      781 aggttgggta gatgttcctt cgattgaatt aactaatgtt gtcatggcag aggcagacct
      841 tatattagct actggtggac ctggaatggt taaatctgca tactcttcag gtaaaccagc
      901 tgtcggagtt gggcctggaa atgtacctgc aataatagat gagagtgcag atataaaaat
      961 ggctgtaagt tcaatcttag tatcaaaatc attcgataat ggtatgatat gtgcttccga
     1021 gcaagctgta atagtaccag aaaaaatata tgaagaagtt aaaaaagaat ttaaatatcg
     1081 tggagcacat ttcctaaata aagaagaaac tgaaaaagta ggaaaagtag taataattga
     1141 tggttcatta aatgccagaa tagttggaca accagcacat gtaatagcca aaatggcgga
     1201 tgtagaagtt ccaaaaacag ctagaataat aattggtgaa gtagaatctg ttgaattaaa
     1261 tgaacctttt gcacatgaaa aactttctcc tgtacttgca atgtacaagt ctaaaagttt
     1321 tgaagatgca gttgctaaag cagaaaaatt agttgcagat ggaggctatg gtcatacatc
     1381 ttcattatat gcagactcta taaatcatcc agagagagta gaaaagtttg taaatgctat
     1441 gaagacttgt agagtattag ttaatactcc atcatcacaa ggtggtatag gtgatttata
     1501 taactttaaa ttggcaccgt ctcttactct tggttgtggt tcttggggtg gaaactcagt
     1561 atctgaaaac gttggagtaa aacatttgct taatatcaag acagtagctg aaaggagaga
     1621 gaatatgctt tggttcagag caccagaaaa ggtttatttc aaaaaaggat gtttaggtgt
     1681 agcagcaaga gaatttaagg atgttatgga taaaaagaaa gcatttatag taacagattc
     1741 tttcctatat aataatggat atactaagaa attaactgat ttattagatg agatgggaat
     1801 taagcatact acattctttg atgtagctcc agacccaaca ttagcttgtg caagagaagg
     1861 agctaaagct atggctgatt tccaaccaga tttaataata gctgtaggtg gaggaagtgc
     1921 aatggatgct ggtaagataa tgtgggtaat gtatgagcat ccagaagtag atttccaaga
     1981 cttagctatg agatttatgg atataagaaa aagagtatat gtatttccta agatgggtga
     2041 aaaagcgtat tttgctgcta tcccaacttc agcaggaact ggttcagaag taactccatt
     2101 tgcagttata actgaccaag attcaggtgt aaagtaccca ttagcagatt atgaattaat
     2161 gccaaacatg gctataattg atgctgatat gatgatggag atgccaccaa gattaacagc
     2221 agcatcaggt gttgatgctt taacacatgc attagaagca tatgtatcta tgttaagaac
     2281 tgaaccagct gacggtctag cactacaagc aggtaaaata atatttgaat atttacctcg
     2341 tgcatataaa aatggaaaga atgataaaga agcaagagaa aaaatggcaa tggcatctac
     2401 tatggcagga atgtcttttg caaatgcctt cttaggaata tgtcactctt tagcacataa
     2461 attaggtgca ttccaccatg ttcaacacgg tgttgcaaat gcattattaa taaatgaagt
     2521 aatcaagttc aattgtgcag aagctccaaa taagatgggt gcattctcac aatacagata
     2581 tcctgattgc atacaaagat atgctgaatt tgcttctttt gcaggtatta aaggaagtac
     2641 agaccaagaa aaagtagata atcttataaa agcaatagat gaactaaagg caaaagtagg
     2701 tcttcctaag actataaaag aggctggagt agaagaatct aaattcttag aaagattgga
     2761 tgctatggtt gaacaagctt ttgatgacca atgtacaggt gctaatccta gatacccatt
     2821 gatgagtgaa ttaaaagaga tatatctaaa agtttattat ggtaaataat atctattgtg
     2881 caacataggt tctattgggt ttgtatataa attattagat ttgaatataa atagagtttt
     2941 attagagaca cgtagaagta actataaata atagattata atttaaattc taaatggaaa
     3001 tgataaaaag tcagtaattt aatggtaatt tcgaatatgt gtatttaatc aattaacttt
     3061 aaaattagtc ggaaaatttt tttattacat ataattttaa agcataatat ataaaaaact
     3121 ttatgaaaaa attataaaaa tattcagaaa attctattga ctaatttatt tatcatgata
     3181 taataagttt agaaaagaaa aaaattgggg cctattaagg cccccttact attttttgat
     3241 agatttattt aaaattactt tctcattttg attttttata tttattatag agtcagtata
     3301 atccacttca aagttggcaa aagcagagaa taaatctata tctcttaaca aatgaattgt
     3361 catacattga ttgttcttat ttagccttag aatcaatgct cttacaaatg gaaattgtat
     3421 tctagaatta aataagttag aaccatcagt atctatatct aagctacgat aattaatatc
     3481 tataaatata tctacatctt cattttcttc tcttattatt ctgtagtcaa aatccaagtt
     3541 taaatttaaa ttacctaaaa tcaataacat ttaaatcctc ttttctataa atagttttag
     3601 agtgttaatt taagctctcc atctattata gatataattc tatctgcttt tctagcaata
     3661 ttttcattat gtgttattat tataagtgtt tggttatatt tttttataga actttttagt
     3721 aaatccatga cttcttctgt agttttactg tccaaatttc ctgttggttc atctgctagg
     3781 attatacttg gttttgcaga taaagcacgt gcaatagcaa ctctttgctg ttgaccacca
     3841 gataattggt tgggaagatg atttttcctt tcattaagac ccaataaatt tataaggtca
     3901 tcaatatatt ctttatctat ttttttattg tctaacttta gtggtaattc tatattttcc
     3961 tttacattta atacaggaat taagttatac tgttgaaaga caaatcctac cttttgtctt
     4021 ctatatcttg ctaattcttt atctgaaaga caacatatat ctttaccatc tacctttata
     4081 gtaccattac ttggtctttc aagaccacct actaaatgta gaaatgtact tttcccactt
     4141 ccacttggtc caattatagc aacaaattcg cctttattta tttttaaatt tgcattcttt
     4201 agtgcatgta cagatgtttc cttttttcca taaatcttac ctaagttaat agtttccaat
     4261 ataggcataa ttattcctcc tattctgtaa tgtttatagc ttcaactata ctaattttat
     4321 ttatctttct tgatggaata tatgtagcta gaatacctac aattatattt gctacaacaa
     4381 ctagtatata tattttgtaa tctattgaaa atcccaatcc atatgataca aagccctgca
     4441 tataatacat tttaaattgt attataattc ctgacacgat agtaattata cttgagagta
     4501 caccatataa tattccttca taaagtatca tatttttaaa gcctctctca gagataccaa
     4561 tagctcttaa cattccaaat tcactagttc ttgaagttaa gttataactt atgttgttta
     4621 ttatgttaag tacactgatt atgaacatta ttgctactat accataacta taaatcatag
     4681 ttctagatgt catcttatca gaattttctt tttctaccat catatctact gttgtagttc
     4741 caggaacttc acttccaatt ttacctaaaa ggttatttat ttttttcttg tttgcatttt
     4801 cattaaggtc tgcataaact aagttatact tatctacacc agttaatttt tttaggtatt
     4861 cgctactcgt tattacatct acaccatcat ctccagagta cataccatta tctgcaaaag
     4921 gataacttat aatagctcca actttaaatt catattctaa gtattcataa ttatctttta
     4981 gactttcata aagacctgta tccatcccac cctttggata tttgactttt atagtatcac
     5041 ctacttttat atctacagct ggtgttcctt caccaggagt aaaagtcttt gtgtatttct
     5101 gtgttttaga aatttctggt acatatacaa tagctaaatt ttcttttttc atttttttca
     5161 agtttatatt tccacttact aagtattttt ctaatgactt tatcatctca tcattataac
     5221 cttttagctt ttgctttagt gtatatttgc cagtagtctt attttttatg aacagaggat
     5281 ttagcttata ataaggagtt gaatttatag cttcgtagta atctaatcta tttattttat
     5341 ttacctctat tcttgtgttt aagatactag cagttttgac tttgtcaatt ccatctatat
     5401 tttttatttt atctatctga ttatcattaa acccttttat atctttaaaa gaagtattgt
     5461 taccaatttc attgagataa acatttgata gaataaaatc tgcattcata tatgtttgtt
     5521 cttgagtaga tttgttttgc acatctaaaa aagaatattt ataattgtct tttatgacga
     5581 ttatacctcc tatactcata gataatacaa ttataatgaa ccccttttta tttctaaaga
     5641 tgtttttcgc agatatagat tttgttactg acatatttaa agttaatttt gatattaaat
     5701 aattaacttt ggttacattt ttatcagctc caaaagtttt tctgatagca tcaataggag
     5761 aaattcttct tatttttaag tatgtaaaga aacttataat tagtattatt agtattgtac
     5821 atgcaattga taagagaatt attttatctg gaataacaaa aggtgtaaca tttatatttc
     5881 cttcaaattg aatatttcct acagacctat taaatatttg tgcacctccc atacctatac
     5941 atatacctat aggcattgct attagagcaa gtatgaaaag ttcataaagc ataagtttga
     6001 aaatcctaaa gttggtggaa ccaatagctc ttaagacgcc atattcctgt actctttggt
     6061 atattgatat aacatagata ctatatatta caataccagc aaatagactc atagcaattg
     6121 caacgtattt acttgaatta tctaaagtac cgtttttcat agtagattct ataagcattg
     6181 agtttcttcc tatacttttc tcatctaaca tagcattttt tattatattg tctatgtttg
     6241 tatttatatc actgccttca ttaaattcaa tataagttgt aaaatcattc agttctgatt
     6301 cattgagatt agaaaaaatt tcacattgtc cctttttttt ctctataaat ctatcttcaa
     6361 ggattccaac aactttaaaa gtttctggat tttctttttg atataattta aaagtaaggt
     6421 tttgatttat ctctggtttt aagcccatac tatttaaaat ccatttctcg actaccacct
     6481 cattgctagc ttttggaaat cgacctttta taagtttaga atgactagtc aaataatttt
     6541 tatttgtata tagtatgttt ataggtaatt tttcattaac atctgtagac gcatagtaag
     6601 atgttatacc cagattttta atatctttgc tagattttat ggactttaat tgatttttct
     6661 ttatattttt aaagtataca tggtcagttc caagctctct tttcaatcta tctacatctg
     6721 cttgctgagc acttcttgag agagtaccaa taccaacaac gagtgatgta gcaagaatta
     6781 tactcacaac aattgctaaa gacctagctt tataactttt tatatacctt agtactattt
     6841 tcaaattatc tttcatattt accacttcct ttcttaacta aatcaattat atatttatct
     6901 aaaatatata gcaaagtaag tattttcaaa ttttaagtag ttaattgtac aaattcatct
     6961 gtatttttac aagtttaatt aatatatatg taaattttaa ctttttacat aattttttta
     7021 aaatgatata atccctatat aaattgtaga ggggagggga atcatggata acaattttgt
     7081 atttcctata gtaatactaa ctttgtttat atttagcaat cttataatga ataagtataa
     7141 attttacaaa gaattagaca tattaaaaag aggcatatta acatttttta tatcgtgttt
     7201 tgcattgatt ttatttatgg agggcactaa atatatacaa ttaaggttag gatatagttt
     7261 tttacttgaa ggagtattaa atattatact ttttttggtt ttaattattt taagttttta
     7321 tacaggcttt aaagttataa gattgataaa taataataat gaacaggaat ataaattaca
     7381 aaaactagat agtaaaataa atcataagga taatataatt atcaaattaa gaagtcaaaa
     7441 acatgactat ttgaaacatt tgcaagtagt gtattctcta ttaaatactg ggctagatga
     7501 agatgccaaa gaatatataa tgaatatatc aaatagcttt aaatcaaaaa attcaaggta
     7561 tggaaaagta tcttatttag atgctatagc ttcattaaaa tacgaggaat gtcttgaaaa
     7621 ggatatatct tttgatgtct acattcaaga ttttatggat aatttagaga ttgaacctaa
     7681 tgatatgagt tcagtactat taaacataat agataatgca atagagtctt tgtctactat
     7741 aaaaaaagaa aagaagtata taagattaca tgcatatgaa gatgaatttc agtatattat
     7801 agctataaaa aataatggtc ctaaaataca taatgttgaa agtatatttt ggaatggatt
     7861 tagtactaaa gaaggacaag ataggggata tgggctacat ttagttaaaa atatattaga
     7921 taagtatgat tatagtatac ttgtaaatag tgatgatttt ttaactgaat ttgtaatctt
     7981 aatcccaaaa tattattagt taggaggaga aaagtctatg ataaaagttt tgatttgtga
     8041 tgatgataag attgtaagag atgatatagc aaatatagct aaagagtctg gatatgtaga
     8101 ttatgttaaa aaagttaagg atggtataga ggcaattgaa tttataaaaa atgagaaaat
     8161 agatttattg attattgaca ttgatatgcc atataaaaat gggattgatt ctgctgaaga
     8221 aattctttct atagataagg atatacatat agtatttgtg acgggtttcg ctgattatag
     8281 tcttgaaagc tttaaagtgc atcctataga ttttgtagtt aaaccattta aaaaagaaaa
     8341 aatacttgat tcaattaaca tagcaataga ccatattaac tcccataaga ttgctaataa
     8401 taattttata gaagacaccc tttttgtata taaaataagg aagcagatac atatgattaa
     8461 ttttgatgat atagttatgt ttgagaaaaa ttcaagagca attaatctac acactaaaga
     8521 tgaagatata ataaagtttt atgagaactt tgatgattta aaaaaacgcc tacctcctaa
     8581 tttctttatg acccatagac aatacatagt aaatctaaga tttatacata aggttatacc
     8641 tataaataaa tcttcattag agataaggtt tataaatttt caagaaaatg ccatattaaa
     8701 caaatctcta gaaaaagatt ttttatatag attttatagg gttaaaagat tctagtgaag
     8761 atagaagaaa aatacttttt gtgtgatata ataagtaccg taaagataat gtataagaga
     8821 agtttatata aaaattattt gtaattatga tttaatctat ataaattaca gtatagtata
     8881 tattggactt tctaattttt ttaagaaagt ccaatatatt ttagagatag tttttatact
     8941 tataaattac ttaagatact agggtcttta atcttactat cttcagcaag taatagtatc
     9001 ttagacataa tctcagcagt tttagggtct tcatcagcga atggaaggaa tatcttacct
     9061 ctactttgag aatgtatagg aagtatattc aaagcaccca tccctttttt atgtacaata
     9121 ccagaaccca tatgcacaga atactcacct aaattacctg ctataagagc atgagagcct
     9181 tcaaaattga cattgttaat ttttaataat aataaaagtt ctttagcaat tgctatacgc
     9241 atctctacag tagaatggct agtttcaggg tcaacaccac cagcatgagc gacacttaca
     9301 acaaggtcta tatcacgcat aacttctgag aatagaattg gaggtataga agctaattct
     9361 aaacttttat aagtttttct atcaaaaaat tctactgttt caatagtagg aggctctata
     9421 tctgctggtg aaaaccaatc tgcaagtgca aacatctttg ccactatatt ttctttataa
     9481 aatactttct gtaatccctc ttcataatcg acagtccaaa gctgatttct aagaagtgct
     9541 acagttttct ttggctgaat ttggtgacca gcatatctgc gagatatagt tttttcagat
     9601 aattcatcac tgtttattct ataatactca cggaatactt gcttaaatgg ttgaacaatt
     9661 tgcttactaa ataaatcatg ttggaactca ctccacttac cagattcaaa caaatgaact
     9721 ggatgtgcaa gttttaattt attttttgcc gtcaacttat aagtttcacc ttctatattg
     9781 caaataaaga acttagactt atcattataa acatatccaa tattatcatc acatacccat
     9841 actagatttt taatcaaagg gcagataact ggatttttat agatattggc aatttcttta
     9901 actgtaaata ctatttcatt tttcatagct tgttctaaac tctgttttgc acgagaatac
     9961 tgactcttta aatctttttg gacagattta agttcaagta catatggatg tttagcaata
    10021 gcttttggta tactctttaa agacttgttg cctttttgaa ctttagtaga tgcttttccg
    10081 ttttcatcaa catctatcca aattgaagta ccatcaatat cttttggttt aaatagatga
    10141 gcaacttcat ttagtttttc agtctcaaga gcccacatca tacgatttac atctccaaat
    10201 ccagcacttc tagctagatt ttctagcgct atagtacaag cttttgattc actttcacga
    10261 cgttgtgcac caaattgctt gctttcttta gcaaactttt gaataaattc ataacggaaa
    10321 gttgcttctt ttaggtctcc ttttttcata ggaagtaaag agaaacttct caatttatct
    10381 tgatttctat ttgtttcaat ttctttcatc aacatgtctt tatcaagctt accaagtgta
    10441 gcatcagcat agagttgtgc acgtctatga gagttaccac cactagttat atatttagct
    10501 gacttgtaaa gtatattgaa tctttcttcc ccaatagttt tatatgaatc taaaaaccaa
    10561 tctacatcaa atgcaccatc aatgaaacta cgtggtgaaa taggggagaa tagagcaacc
    10621 tctgattctt tttgtgcaga gaagccttca tttatatgtg catggaagta ccaaattgta
    10681 cttttcagac ccttccagtt taaatatttt tcaattatgt ttacccattg aggggcatac
    10741 atagcagatt ctatcaatct taactcagta atgtcggtat ttttaagtaa actttttaat
    10801 ttttttgaat catcttcttt agtaggatag caatttttaa gtagagaact taaaacatct
    10861 ttttttgaat aattgctacc atatgaatat cctctctgga aagtttcttt accaagagcg
    10921 accaaaatat ttacaaaata atttatacct tcaaagtatt gtatattatt agctagatta
    10981 gacacttctg ttggcatatc acctctacgt gattcaattt ctataattcg gtttgtaaca
    11041 gtttttaaat tatctttaag ccaagggtac ttttctaatg tgtcgccagc ccatctagga
    11101 tttgtcaatg ttcttattat atttgatgcg ttgtatccac ttatcatttc tctattaact
    11161 tgctcttcag tcattaatcc taattcatgt gcacgagcaa acacatttat gctagtatag
    11221 aaaggaaact tatattctag caactgataa tttgaaaacg tagtgtagaa aaacttagta
    11281 aaagaaatat catcataaat aaacgaatgt gctttatcaa gccaatagtt taaaataggg
    11341 gcatttaaag cagggcagta ttcattttgt ctgtaatagt ttttagtaaa ttcaactctt
    11401 tgttttttac attgttctat agtcaataag tttctaagtt tatagtatat ttctaaacaa
    11461 tagttaaata tttctttatg gtcaaactca tttagtaaaa ctcttaaaag ttttcttgta
    11521 agtccattaa atttaagttt ttcaagtttt gtatatattt tattttgttt tttagagtca
    11581 aggacatttg aatatataga tgaaaaccaa cttttttctg tttcattata tccacatgta
    11641 cttatactat aaaataagat atcaagtatt aatttgtaat ctaagtcaag ttttttacta
    11701 gtttctctcc aaagctctgc taatggataa ttatcaagag tatcattaga aactttatat
    11761 ttaataggag tcaagttata agtagaataa cctaaaagta ctttgtctac attacctgac
    11821 catctttcaa cttcatattc ataatctgca ttttcagtaa taagttcatc aaatttttca
    11881 taaactttta ttattttatt tatatccgta tcgagaattt tttttgcttc tttttttgtt
    11941 gctacagaaa cagaatcctc acgagtagat aaacttatat ttgagataat tttattaagc
    12001 tttgatgagc tattagaatc atctttaaaa tcaaattgtt ctggaatttg aactttgttt
    12061 tcagggttat atattccaaa accattttct ttgcagtaat cattttttgc tttacctaac
    12121 aaattatcaa ttagtatttg aacttgagat gtattctttt tagatttatc aatatcctct
    12181 ataagtaaaa taagcttatt atatagttct tgtctagcag tattgtcttt taataagttt
    12241 acaatttcaa gacctccaag gcatttttga gaatttgaag attttatcag tgaagaagca
    12301 atattttcca aatttgaatc atcttgttca agtaaaattt ttattactgt ttgtctaata
    12361 gagcttgtct ttaaagacaa tagattggtt atcttatcta attctacatc tgttagctca
    12421 agctttctaa gtagactcaa taccgtttct ctattggaaa cactcttatc acttaaacat
    12481 tccaataaaa attcacgttg tctatctgtc tttggttcat ttacaaaatt gactataagt
    12541 gaagctctta aatctgaact cataagatga caattttcca taagcacatc tataaattga
    12601 gcattcatat cgtatgctgt taaagtcatc atacatttaa gtatttcatg agaagaaatt
    12661 tcaatccaag tccatggaaa tacacttcct gaaaatgatt ttgtctttga atttaaaatt
    12721 tctaaagtat gttttaaact ttcaaattgt tcttttctag catcaaagtc tttgtcaata
    12781 ggagtgtttg acaagtcaaa attagtttct ctgttttgcc aactatagta gtagccaaca
    12841 gaaccttcta catagaaatt tgtaattacc catgctagta tctctaaatc atcaccctta
    12901 atgtttttag atgctataga atgtttaaaa tatgtacttt ctgtatgcga taaaaagtaa
    12961 agagctacaa gttttttata tttttcctga caagaaagta agttttcaat aagagagtaa
    13021 gagtcatata tgttgtaagt tgcagtagcc cataaggcaa aataaatttc aagattatca
    13081 ttgctattaa ttgattctat acggaacttt tcatcagtca atgatttata tgaaagttct
    13141 aaacatttac gtattattcc aggtttttgg tctgaaaaac caagtcctgt ccatgtatcc
    13201 aaggctctta taacagaact aaatcgctct aaattgttgt ccaaaatagt ttttaacatg
    13261 tatattgatg cagaaattgt accctcatct aaactctcta caatttgctg tctaagtcct
    13321 tcttgttgtt tggctgccaa aagaagttta gaaagcattt catagcattt atcactatga
    13381 ctcttcatga taccaattat catttgtctt gtaagtagag cagtttgatt ttctccaaat
    13441 attacttctt ctaaagcact tattactttt tgatttcctt tgtctatttc tagtgctatt
    13501 ttatcactca ttatagagtc atagtacaag accttatttg gaagagtaag ataatctata
    13561 actgaaaaat cataataaat taaatcaaca tagtttgaaa taaaccttat aatacctcta
    13621 aaataatcca caagtttatt tgagcgatac cctttacgat aatatcctcc tgaatatgta
    13681 taatccatct ttcttttaat taattggcaa agttcttcta cttgttcaga agtaaatatt
    13741 aattctaatg cattttttaa aggtccagaa aggagctcgt ccatattttt aatttcgtag
    13801 tagtggcaat tgtagtattt ctttatcata tttaagaatt tgtcttcagg ttctctataa
    13861 tagtatgaac tattttctat gcagtcccaa agagactttg ctaagtcgta gtactgtttt
    13921 gaagaagatt tagtaaaatt atttttaaaa ttttcaatta ttttaggatt tttttctact
    13981 ctagtacgtt ctaacattta tgtacctcca aattatttat tttaaaacat tcttccagca
    14041 agcaaggtaa gtcttataaa agtaaatata tctccacttt tcagatttat agaggaatcg
    14101 agtttttcaa taacatcctc atttataagc actttgtaga ttccatcttc aaatgcctgt
    14161 aacgctacct ctattgcctt ttctacagat tgtttattct cattgtaaat agagccaaat
    14221 ccaacttttc caatatcagc ttgagattca atttcttcat tagttaagaa gtttattatt
    14281 gatttttctg ggattttctc attatattca agaacatttt tagatacaat ttcagagata
    14341 gcttctttta ggcaattgat gttgtcagat acatcaaaag gggtaagttc tagcataggt
    14401 ttgcgtttgc caacagcctt aactctaaaa taaatttgca taaaatcacc tcgcaaaata
    14461 ttaattatgt atttagaaat ttaaaaattt atctatttgt tatattatat cattttgtga
    14521 attattaatg tgaattatgg aattatttta caaaaaatag aaaaaaagct atcttaaagt
    14581 ctaaaaaagc aataaaagat aacttttaac tataattata tagaattata actgtacatt
    14641 tattaaattt taattttaga attatttttg ataactatct ttttttattt ataaattttg
    14701 ctcctaaata ttttttgata tcttcttcag gagaaaagtc atcaataagt atttctttcc
    14761 cagtgtcttc aaatactaca agaacttcac agtcaccttg taagaaaaat tttctaactg
    14821 tatcttcttt aacttctcta acttttatta cattcttttt ttctttttca aagtcttttg
    14881 tgtaataact tcccatcata ttaattattt ttgtatatct attcataaaa tacctccata
    14941 tgcgattaaa tttaaaaaat atgttataat aattgtttgt attaacttaa taattataac
    15001 aaggagtaat aataatgact agtataaaaa ataaagatat tttaaattgt atagattatt
    15061 ccattaaaaa taagctcttt gacaaactaa atgatgtata taagtccttg ccagtaggaa
    15121 attgttctgg atgtggaaat tgttgtatgg agtcagtagg tataaattta attgagttct
    15181 tgaatatatt ttgttatctt gaagatagag ttgatttaaa aaaaagctgt ataagtaaaa
    15241 tattagatta ttattttgag gagtatacaa gaaagaaccc atgtccattt aaagatacta
    15301 ataatagatg tttaatatat gaagttagac cattaaattg taggatgttt ggacattgga
    15361 aaaaagagga ttataataaa aatttagata atgttattga gaaaaataat aactatagag
    15421 agttaatgaa aaagcaatat ggatttgaaa taaatgatga agtagtaaat tacaggatag
    15481 attattgtga tagatttatt ccaaataaag attatttaag taaatctaag agattaagct
    15541 tttttgatga gcttatgata ttagactcaa atatttattc aagtggaaat atagatatag
    15601 actttaggga tagaggtata gttgaatatt ttatagagtc attactttat agaaatttag
    15661 catataatgt gaaaataagg atatctaaag aatacaaaat aagaaaaaga actatagata
    15721 gaataaaaag gattgttctt gtatagtttt atattagtaa gtaataaatt gtatcataaa
    15781 tattgtcaaa atgaggtgaa gaaggtgaat cttacaaaag taaaaggaaa tacctttttt
    15841 ataaaaggtg gcacgaatac aggaatatat gtatttgaag ataacacagc acttatgata
    15901 gacccagggt tatgtggttc aagacctagg agagtaaata aagtattaga tgaaaataat
    15961 atacaggtta gatatattat aaatactcat ggtcatgatg accactatgg agcttgtaat
    16021 caatttaaag aatattataa agatgtgtgt ataatgtcat ctgaatacga taaattatat
    16081 atagaaaatc cagaattgtt ttcaaagtat attgttgggg gaaagagcaa tgtatttatg
    16141 gataatatct taaaaaataa aatgttggat gatataaaaa tagataaatc tatagaagct
    16201 ggaaaaattg aattaaacaa tgaaatgttt gatataattg aatttcaggg tcatacacct
    16261 ggtagtatag ctgttttgac gaaggataat gtgatttttg ttggcgattt attgatagga
    16321 gatgaaattc tatcaaaata cgatttcttg tttctatatg atatacaaga acaaataaat
    16381 tcattagaaa aattaaaaaa tatagatttt gagtacttag ttttagggca tggcaagcaa
    16441 gttatatcga aagaagattc ttataaatta atagaaaaac atttgaatgc tttaaaaaaa
    16501 tatttatatc aaataagaga gttgcttgta gaaccgaaat ctttagaaat tttattaaaa
    16561 aacattataa ataacaataa tttaagcaat aattataaag agtatcattt ttttaaatct
    16621 tcgttagtat ctgtcataag ttacttagtt gatttagaag aaatagggta tatattggaa
    16681 aatggagaat tgctttacta tacaaaaaca aaataaatta tgataaaata aagaaggtca
    16741 taaaaaacaa ataaagggtg agcaatatgg aatacgaaaa atggaatgag atactagcac
    16801 cttatgaaca tgcagtagag gaattgaaaa taaaatttaa aaatataaga aaagaatatt
    16861 tagcaaaagg tgaatattct cccatagagt ttgtcacagg tagaacaaaa aaaatatctt
    16921 ccataatatc taagttaaaa aggataaatg caaaagatat agaaactgaa attgatgata
    16981 ttgctggtat aagaataatg tgccagtttg ttgaggatat atatgctata gttgatttga
    17041 taaaagtaag aaatgatatg actataatag gagaaaaaga ttatattaca aattacaaag
    17101 atagtggtta tagaagctat catgttataa taaaataccc tataaactcc attgcgggtt
    17161 caaaggaaat tatatgtgaa atacaaataa gaactcttgc aatgaatttc tgggctacaa
    17221 tagaacattc attaaaatat aaatatgacc attacatacc tgagactctt gctgaaaggc
    17281 ttagaagagc atctgatgct gcctttcttt tagaccaaga aatgagtgaa ataagagagg
    17341 acataatgaa agctcaggct atgtaccaaa tgaagtctat agctattaga gatactttaa
    17401 atagaataca agagttatat gacttgggtg atacacataa agctatgcag taccaaagaa
    17461 aacttgatag aactgaaaca gataaaaata taactgaaat tttggaatta aaacaagaaa
    17521 tagatatatt attagagcaa tataaggata ctaaggtaga tgataaagta gttcagtaaa
    17581 aactacttgt agaaaatttg ctaaatatta atggggctgc ctcttaatgg aaaataattt
    17641 ctaaaaagag acagcctatt tagtgtaatt aagaaaggaa tgatattatg agtttgtatg
    17701 ctataggtga tttacatttt tcaacatcag tcaataagcc aatgaatatc tttggaagta
    17761 attgggatgg tcatgaaaag aaaattatag ataattggaa agaagttgtt aaggaagaag
    17821 atatggtctt agtactagga gatacatctt ggggaattaa tttatccgaa gctaaaaaag
    17881 atttagatat aataagcaaa cttccaggtc agaaaatact tataaaggga aatcatgatt
    17941 actggtggac aaccgttaca agtttaaata agctatatga agacatgcgt tttattcaga
    18001 caaattttta tgaatataaa gattatgcaa tttgtggagg taggggctgg atatgtccta
    18061 atgatgttaa atttgatgag actgatgaaa aagtttataa aagagaagaa catagactta
    18121 gactatcatt agaatctgct cgtaaaagtg gtcactcaaa aataattgtg attactcatt
    18181 atcctcctac gaatgataaa ttggaagaat ctttatttac aaagttattt gaagaatata
    18241 atgttgaaaa ggtgatttat ggtcacttgc atggtaaaga atcttttaaa atgggattga
    18301 aggggattcg aaatggagta gaatatacat tagcatcttg tgactataca gaatttaatt
    18361 taataaaagt tcacgattaa aaaacataaa ttggtaaata ctataagata catgattaaa
    18421 aataaatatt tgagggattt cggttttgag aaaggagaca cgacttatga ggtggatatt
    18481 agtttttata tttttatatc ttattccttt agtagtgata ttcagaaatt acaagaattt
    18541 taagaggtct tgtatctaca gtagtattta tgtggtatta gctacaacaa taatgataag
    18601 taatatttat atgagtggac ttaataaaat aaaagagtct atgtattatc acaattatat
    18661 ttctgatgag acatatgatg aaaagtatgt atcaaatttt aaggaaaaag aaagtactga
    18721 tttaaaacag gataataaaa agaatgaaga aatatcaaat aataaaaatt taaacaacaa
    18781 acaagataga aatatacaaa ataaagaaag taatgaagaa ggaagtaatg atataaaaga
    18841 aaagaataat tctgatagta atgtttcagt gaataataaa tcagaaaaac aagaagataa
    18901 tacaaaagaa aataataaat atacaagtaa agaaaaaaat agtgaagata taaaaaaaat
    18961 agatatggaa agtataggaa tttttaagaa agaaatttat aatatagaaa gagcagcttt
    19021 agttccaatg agagaatgta taccttacac taaggatata gcaaaaaata taactaaatt
    19081 atctagtata aaaaaagatg ttacaaatgc cagaaacaag tgtaaagaag ttgtaaaaga
    19141 atatgaaaat atgcagattc cagaactttc taaggagaag tatacagaaa cattgagtaa
    19201 tgctagagat gacgtaaaga aagcatatga gcttagagaa aaatctatgg aaagtgcttt
    19261 gaaattaata aatactaaaa gccctaaata tatagggaag ataacagaat atcttgattt
    19321 atcagataag cagatagaaa gttttaaaaa tagattgaac gatttaaaag aagaaataaa
    19381 taattcagaa acaaataata ttaattaata agatttaaag atataagtta aaagggagat
    19441 tatgatgaat aaacagagta taggaaaact aaatatgttt tttatgggga ttactcaaag
    19501 aaaccttgat aatagggatt tttttattga gttagaggtg atttttaaat ctggaagaaa
    19561 agagtttaaa ggaattgcaa tagaggaaga taataaatat aaatttaatt ttaaaggaaa
    19621 atcagattta tatacgttta atgagttgct taagaatatt tctaaagagg cagaaaatta
    19681 tgatggatta gtatttaaat atgttgagcg aggaactatt gttgtaattg aaggagacaa
    19741 taagaaggta aatgtaaaat atttagataa taaggaagaa gtacctaaaa tagatgaatt
    19801 tacggcatct caaataaaaa acagagatta ttatgtaaaa gtaggacaag caaatgcact
    19861 tttaaaagaa attggagtac ttacaaaaga tggtaagata aaaaatgata aaataagaaa
    19921 atacaatcag atagaccatt ttgttgaatt gatagatagt attttaaaag aaataaagga
    19981 taaagactgt ataactatct tggattgtgc ttgtggaaaa tcttacttaa gctttgtctt
    20041 aaatttttat ataaaagaag tattgaagaa aaactgttat tttataggaa tagattattc
    20101 agatgtagtt atagaagcat caaaaaatat ggctaaaaac ttgggttata aaaatatgtc
    20161 atttataaaa gaggatttga ctaattatac acctaatagg gatgttgacc ttgtaataag
    20221 tctacatgct tgtgatacag ctacagatat ggcaataggt ctaggaatta gagcaaaatc
    20281 tgaagctata gtagtagtac catgttgtca taaggagtta ttagggcaat atagatatga
    20341 agctatggaa ccaatattaa aacatggtgt atttaaagct agatttgcag atttaataac
    20401 tgatggttta agaacattgt tattggaggg caatggatat gatacttctg tagtagaata
    20461 catatctcca cttgatacac caaaaaattt aatgataaga gcgataaaaa ctaaaacgaa
    20521 taatgacaaa gcattaaaag aatataaaga actgaaatca caatttggag tggaaccaac
    20581 cttagaaaaa cttatatatt agttaaaaat gtaaaattta cgaaaagctt gcttaaatat
    20641 gcaagctttt tggtattata taggtatagt catatccaga gttttgattt ataattaaag
    20701 ctgtgctata atatttgatt aatcaaatca ataaatgtta catggaggta aaaatgaaca
    20761 aattgaatat atgtattgat atagatggaa caataacaag cccttatcat tttcttccat
    20821 acctaaatga tatctttaat aaaaatataa ctgaagcaga gtgtataaca cataattggg
    20881 aagaattgta tggttcggga ataagagatg tgtatgcgga gtttaataat aagtatatac
    20941 actcatatga tgaagcaaag attgtagagg gtgctacaga ggtaattagc acattatcta
    21001 aaggacataa tttatctttt gtaacggcta gacatgagtg tttaactgat gtaactaaaa
    21061 attggctatc gagacaagga ttttcagata tagatgttta cttattagga agtgattata
    21121 aagttgaaaa ggcaagagaa ctgagttgtg acatatttat agaagataat cctttaaact
    21181 cagtgcaact agctgatgat ggtgttaaag taatacttct ggatactaac tataataaag
    21241 atgttaaaca tcaaaatata gttagagtta gtaattggat agatattgaa aagattataa
    21301 aacaaggtat ttaaagattt gtagattgaa agggggagca tatgatagat ttaaaaattg
    21361 aaaatagaaa aggaaaaatc catgtaaaga gcagtgaaat aaaagacatt ttagaattac
    21421 gtccagactt cgaatatgta caagatatat caaatactat aaatcaagaa aatatattgg
    21481 tatttgattg ccagttgaca ggaaattctt ttgatatgga tgatttagat atagaagaaa
    21541 tattggaaga gttaggtgaa gaaatagatg agtcttattt cagtgtttta tttgaagatg
    21601 taagagcgta tttaaaagat gctactgatg agatagaagc agacttacag gataattact
    21661 tatttgataa tttgagatgt tattttgata tatacaatat aaatcaagaa tttacagatt
    21721 ttaagtttgt atttttagtt tcttttaaag atataaaaat ttcttcatta gcaaacctag
    21781 ctaagattgt ttctaagaga cagcttatag gtgcatctaa gttttatagt taaaatgaaa
    21841 tattattata ataaaagcta gtaaccttgt aaaaatatag gttactagtt ttttatttat
    21901 tcattttcac aaaatttcag aaaactattt acattctaaa aaaagtaatg tatattaaac
    21961 ataatgtaca ttataatgga taaaaagttt ctattaaatt actaagttta tttaataatc
    22021 aaaataattt atcttatatt agtatgtatt agggggtatt tatgcaaagt ttaaatgaaa
    22081 taattgctga aaatttaaaa aagataagga aagaaaaaca tcttagttta gacaagattg
    22141 cacaacttag tggtgtaagt aagagtatgc tatctcaaat tgaaaagtgt gaggtaaatc
    22201 ctactatatc tactttgaag aaaataacta atgggctaaa aatttcattt acatcattga
    22261 tggagaggca ggaaagtgac atagaattga ttcaaaaaag tgatatagac cattttattg
    22321 aagataatgg aaagtatata agttatccaa tatttccatt tgactccaaa agaagatttg
    22381 agatatttat gattgaaata gaagaaggtg gaaatttaga ttcaaatgct gaacttccag
    22441 gaacacaaga gtttataact gtttttagtg gcgaagtaac cattaaaata aatggagaag
    22501 attacattgt atctagtgga aattctataa gatttaaagc tgatgtatca catatatata
    22561 gaaactcagg caaaggtata gctaaaatga gtatggtagt atattatata taaataattt
    22621 aatggaatta gagtgatatt tagatgattt taggtatgaa gatatattat atttaagtta
    22681 taggagtgtg atttatggat gaataataaa aaagcagttt ttttcttttg cagtgattta
    22741 aagaaagacc cagtagccag taatgtgata aaatgttgtg aagaaataat ggatttaaat
    22801 caaactgata ttgttattga tgataatttt gtattagagt ttaaggataa aaacaataac
    22861 ttatttcatt ttgtaaaaac aaaagatgta ataagtcata attataaata ttatcttcca
    22921 attttaaata gacattttag agattatgat tttgcaggag ttgtaaattg gcatgaggga
    22981 gaaaatgctc cagaacatat cctaacagct catacaattg gagatgttcc aacaggagaa
    23041 tttggaaatt caaatccaag atactttaaa aatctaatca atgctattga ggatataaaa
    23101 aatgaaaatc tacttgatga atttactaca ttaacagaag caactcactg gtctggaaca
    23161 acatatggtg aagaatctaa attaattaca gaatattctg tacctatgtt ggatattgag
    23221 ataggaagta gtagtgatag ttttaataat agtatagcaa tacaggtatt ggcaaaatca
    23281 ttaattagag tatttgattg tgatgaacct ttgaagactt tgttatgtgt tggaggggta
    23341 cattttgaga aatctttctc agatattata aaaaataaag aatataacat atcaattgga
    23401 catgtattgc caaatcaatg gattgtaagt ggaatgtatg atgatgaatc tggatttgag
    23461 aaactagaaa agtgtataaa tagcattgaa ggaggaattg attgtatagt ttttcatgat
    23521 aaattaaaag gtacctataa agagcaatgc cgtaaactag gtgaaaaact taatgtacct
    23581 gtatttaaac ataaaatatt aaaaaatcca aaagacttac ctatatggta gtcttataca
    23641 gttaatataa caataagtcg gattaatttt taagcgaggt caaaaatgat attatcttat
    23701 tttgcattat taattagagt agtgctatta gcttttgaaa gagtagttgt aagattatta
    23761 ggtgatgaag aaggagatat ttataaaaat atagcttcat catttctatt tttctttata
    23821 gggggagtat gtttacttcc atttagcata atggatagag ttagtgattg gtcgttttta
    23881 ataccatgtt atataagtag tttagtctat tcaattggtt cagtagcgta tgtaacgtct
    23941 ttagcaactg gggaagtttc acttgttact ccaataaata gtttaaattc attgttttta
    24001 ctgcttttat cagttatatt tttaggtgaa ggtttaagtt tagcaaaagt tgcaggaatc
    24061 ataattatga ttggtggagt gtttatatta aaaaaagtaa gttctttttt aaagaatgtc
    24121 tctgtaattt ttaacaattt accatgtaga ttaatgtttt tatacatatt tttgcagtca
    24181 gtaggaagag tattggataa ggcttttttt gtacaagtat ctcctatatt atattcaact
    24241 atactgtatt ttttcgtagg tttaaattta tttatctttt tagcatttaa aaagaaacaa
    24301 aaaataattt gtgagatttt tactaataaa aaggggcttt caattttaag tggtgctatt
    24361 aatggatatt cttatttagc attattaatt gctttaaata atctagaact tagtatagca
    24421 gagccatttt ctcaagtatc tatgataata actttaatac ttgctcattt tatattcaaa
    24481 gaaaatataa aagaaaaaat accaggttca attttgatac taattggtgg gtggctactg
    24541 ttactttaat tgacataaaa aatgaattat aaaggtgcta ttagttagta tcagaaaatt
    24601 taaaaaagta ttgacaattc ttttaggcaa atgttaatat aataatacaa aatagtataa
    24661 atagaagaca tgttactggt aatgcaggca ggatcgaata tattgctttg atttttcaag
    24721 gtgtttatat aggttctgcc ttttttgcat cttgaatggc cattcataaa aaattatgga
    24781 gaaggagatt acaagatgaa ttataaaaaa actgccattg acattattga acaagttggt
    24841 ggttcaaaaa atattttagc tttagaacac tgttcaacta gactacgctt taagttgatt
    24901 gatagaagta aagtcaatca agatgcttta aaaaaagtac aaggtgttat gagtatagtt
    24961 aatggggcac aatttcaagt tgtcattgga aatagtgttg ttgatgtgta tgatgaaatt
    25021 ttaaaagttt gccctttaga tacaaatgat agtactgtaa aaactgaaga aaaaataaac
    25081 ttaggaacaa gatttataga atttattatt aatatttttc aaccactgat ttttgctatt
    25141 gcaggagctg gtattttaaa atcactgttg atgttattag caatgtttaa tattttaaaa
    25201 tcagattcta ctatatatac attattagtt gctatttcag atgcaacatt ctatttccta
    25261 cctcttatgg tagctgtaac tactgccaat gtattgaaat gtaatcgttt agtagctatt
    25321 gccgctgtag gatacttatt gcttccagca acaacaactg ctttaagtga aggtgttgaa
    25381 ctatttggat ttacaattcc aaacattgcc tataatgcac aggttttccc ggctatctta
    25441 tgtgtgtctt tcttggcaac aatggagaaa atttttaaca agtattcacc aaaaccaatt
    25501 cgaactttct ttgttcctat gatgtctttg gcaattactg ttccaattac tctaactatt
    25561 ttaggtcctt taggatataa cgttggtaca atttttacaa ccattatttt attcttatat
    25621 aacaaaatgg gattcttagt tgtaggatta cttgctgctt ttcttccatt tatggttgca
    25681 acaggaatgc acaaagcact aataccttat tcaattaata caattggaaa gttgggttat
    25741 gaagctttat atatgacatc ttcattagcc cacaatattt ctgaaagtgg tgcttgtttt
    25801 gcagtagcct tgcgaacaaa agatgaaaca ttaaaacaaa cagcattatc tgcaggaatt
    25861 tctgccttga tgggaattac agagccagct ttatatggta ttaccttaca acataaaaga
    25921 gcaatgttag gtgttgtatt ctcaagtgca atttctggta cctttttagg attatttgca
    25981 gtaaaaggat ttgtaattgt tggaccaggg cttgccagta tgagtatgtt tattgaccca
    26041 aacaatggaa acaatttgat ttttgcgagt attggttttg ttattgcaat attaggctcg
    26101 tttattatta cactgattat ttggaaggaa gatacaaata cagtagagtc atcaattgga
    26161 gatgttgttg aagaagagct agaagaagaa aaagaagtaa atatcttagt aactccagta
    26221 gaaggaaaag taatagattt aagtaaggtt aatgatgaac tatttgcaag taagactttg
    26281 ggagatggag ttgcaattat accaaccaat ggaaatttat acgcaccttg tgattcagaa
    26341 gttgtgatgt tatttgaaac aaaacatgca attggtttaa gaacaaaaaa tggggctgag
    26401 attttgattc atattggaat aaatactgta agtatgaatg gagatggatt taaaacattt
    26461 gttaagacag gcgataatgt aaaagaagga gatttattaa ttcaatttga tttagacaaa
    26521 atctcacatg ctaatttaga ttcaactgtt atgattgtaa acaataatgg agcagattat
    26581 gcttataaag tgttaaacca gtcttatggc aatgtaaaaa aaggaagtat cttgtttgat
    26641 gtaaagggag gaatataaat atgagaaaat ttccagaagg atttctttgg ggaggagcta
    26701 cagctgctaa tcaatttgaa ggtggttgga atttaggtgg gaaagggtgg tctgtatcag
    26761 atgtagcaaa ggcacacttt gatgcagatg tgaaagatta taaatctaat aatgaaatca
    26821 caacaaaaga tatagaagaa ggattagcac atcctgaaga tgaagtaaat tatccaaaac
    26881 gtcatggtag tgatttttat catcactata aagaagatat tgctctaatg gcagaaatgg
    26941 gatttaagac atatcgaatg agtattgctt ggtctcgtat ttttccaaat ggagatgata
    27001 aagagccaaa tgaagaaggt ttacagtttt atgatgatgt atttgatgag ttgataagtt
    27061 atggaattga accacttgtt actatgtcac attatgaacc accattaaat atcgttttaa
    27121 actatgatgg atggtattca cgacaagtta tcaatatgtt tgttaggtat gtagaaacta
    27181 tctgtgaaag atataaaaac aaagtgaaat attggcttac atttaatgaa gttgattcta
    27241 tgattcgtca tccgtacact actggtggtt tagttcgaga ccgttttaag gataaaaact
    27301 ttgaggaagt tatttttcaa gctatgcatc atcaatttgt agctagtgcc attgcaacaa
    27361 aaatttgcca tgagattatt ccaaattcaa aagtagggtg tatgttaaca aaattaacgt
    27421 attatccata tacatgtaga ccagaagatg ttttggcaac tcaacaaaaa atgagaagta
    27481 tttatgcata tagtgatacg caggtttttg gtgagtatcc agtatattta ctgtcgtatt
    27541 ttaaaaataa taacattcaa atagtaaaag aagaacatga tgatgaaatt atgaaaaagt
    27601 atccagtaga ttttatttca ttctcttact atatgtcttc ttgtgaggca gctgatacaa
    27661 ctggattaga tattacacct gggaatacgt tattagctgt aaaaaatcct tatttagaaa
    27721 tgagtgaatg gggttggcaa attgattcaa taggtctacg tatatctttg attgaattat
    27781 atgatagata cagaaagcct ttatttatag ttgaaaatgg attgggagct aaagatattt
    27841 taactgaaga taaaaaagtt catgaccaat atagaattaa ttatttaaaa gaacacttta
    27901 aatgtatgtt agatgcgatt atcgaagatg gcgttgaact ttggggatat acttcatggg
    27961 gttgtattga tttggtcagt gaatcaacga aacaaatgtc aaaacgttat ggatatattt
    28021 atgtggatgc tgatgactat ggaaagggga catataatcg atataagaaa gatagttttt
    28081 attggtataa aaaggtaatt gaaaataata gtatagattt taaatgacca atagaaggtg
    28141 agcaaataaa atgaactaca taataaagaa agttttgaac tcgagcgttg ttttagttca
    28201 tgatgcaaag tataatgaat ttattttgct agggaaagga attggatatg gtaaaaaaac
    28261 aggagaatat atttgtggtt ccaatgataa tcaaatgttt gtccctgttg aaaatacaaa
    28321 atcaaaacaa tttttagaat taatggatga tattcctgtc gacattttaa agataacaca
    28381 ggaaataata gtagaggcag aaaagttatt gaactcatct tttaataaaa acttatactt
    28441 aatattagca gaccatttta actttgcaat tgaaaggatg cgtaaaggaa taaaaattac
    28501 aaatcgagta ttttgggaaa taaaaaatta ttatccaaat gagtttaagg ttggaatggt
    28561 tgcaatttat atggtagagg agagattggg cattcaatta ccagaggaag aagcagctaa
    28621 cattgctttc cattttgcaa atgctatggc ttcagatgga aattcatatg atacgattaa
    28681 gtatgccaaa gtaattggag aaataattaa tatttttgtt ttttctttga atagaacctt
    28741 agataaaaag agtatgcatt atatgagatt tataacacat ataaaatttt ttgtggaaag
    28801 atttttttca gatagtatgt taagcagtgg tgatgattta ttatttaatc aaatgaaaag
    28861 gtcaaatcca aaagaaatgt taattgcatt aaaagttaga gattttttag aaaagaaata
    28921 tggaaaaaag ttaacaaatg aggaaatagc tttcttggtt gtacatatcg cacgagttaa
    28981 gcaatagagt gaattactaa tattatctta tacgtctaca tggtagaaaa ttgaaagaag
    29041 gtaaagtata taaagaaact gagacatatt attttgttgt tcattatgtt tattttatga
    29101 gtgtatcctg ttaaaaattt aagttaatat ttataattaa aatatacaaa tagttaaaat
    29161 aagtttatgt ggttatgaat ttat
//
LOCUS       DAYATN010000038        28407 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.038, whole genome shotgun sequence.
ACCESSION   DAYATN010000038 DAYATN010000000
VERSION     DAYATN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.038"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            283..987
                     /locus_tag="ACGI8A_001621"
     CDS             283..987
                     /locus_tag="ACGI8A_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0179622.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            989..2476
                     /locus_tag="ACGI8A_001622"
     CDS             989..2476
                     /locus_tag="ACGI8A_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0179623.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            2681..4294
                     /locus_tag="ACGI8A_001623"
     CDS             2681..4294
                     /locus_tag="ACGI8A_001623"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0179624.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(4657..5487)
                     /locus_tag="ACGI8A_001624"
     CDS             complement(4657..5487)
                     /locus_tag="ACGI8A_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0179625.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            5560..7287
                     /locus_tag="ACGI8A_001625"
     CDS             5560..7287
                     /locus_tag="ACGI8A_001625"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179626.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            7324..9186
                     /locus_tag="ACGI8A_001626"
     CDS             7324..9186
                     /locus_tag="ACGI8A_001626"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179627.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            9519..10208
                     /locus_tag="ACGI8A_001627"
     CDS             9519..10208
                     /locus_tag="ACGI8A_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HFH0179628.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            10325..10999
                     /locus_tag="ACGI8A_001628"
     CDS             10325..10999
                     /locus_tag="ACGI8A_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFH0179629.1"
                     /translation="MTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMVYKKT
                     YMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDPMDLV
                     FLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNEDSEDI
                     ATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAVLVSY
                     NGDYVA"
     gene            11016..12923
                     /locus_tag="ACGI8A_001629"
     CDS             11016..12923
                     /locus_tag="ACGI8A_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HFH0179630.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            13376..14065
                     /locus_tag="ACGI8A_001630"
     CDS             13376..14065
                     /locus_tag="ACGI8A_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0179631.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            14501..15040
                     /locus_tag="ACGI8A_001631"
     CDS             14501..15040
                     /locus_tag="ACGI8A_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFH0179632.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            15059..16102
                     /gene="potA"
                     /locus_tag="ACGI8A_001632"
     CDS             15059..16102
                     /gene="potA"
                     /locus_tag="ACGI8A_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HFH0179633.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            16104..16940
                     /locus_tag="ACGI8A_001633"
     CDS             16104..16940
                     /locus_tag="ACGI8A_001633"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0179634.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            17045..17719
                     /locus_tag="ACGI8A_001634"
     CDS             17045..17719
                     /locus_tag="ACGI8A_001634"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0179635.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            17719..18771
                     /locus_tag="ACGI8A_001635"
     CDS             17719..18771
                     /locus_tag="ACGI8A_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0179636.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            19001..19849
                     /locus_tag="ACGI8A_001636"
     CDS             19001..19849
                     /locus_tag="ACGI8A_001636"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0179637.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            19839..21503
                     /locus_tag="ACGI8A_001637"
     CDS             19839..21503
                     /locus_tag="ACGI8A_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HFH0179638.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            21552..22808
                     /locus_tag="ACGI8A_001638"
     CDS             21552..22808
                     /locus_tag="ACGI8A_001638"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0179639.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            22831..24912
                     /locus_tag="ACGI8A_001639"
     CDS             22831..24912
                     /locus_tag="ACGI8A_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFH0179640.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIIVMIL
                     SMLYIKKYKRR"
     gene            24949..26034
                     /locus_tag="ACGI8A_001640"
     CDS             24949..26034
                     /locus_tag="ACGI8A_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFH0179641.1"
                     /translation="MRKYMNEFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            26088..27212
                     /gene="wecB"
                     /locus_tag="ACGI8A_001641"
     CDS             26088..27212
                     /gene="wecB"
                     /locus_tag="ACGI8A_001641"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFH0179642.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            27290..28264
                     /locus_tag="ACGI8A_001642"
     CDS             27290..28264
                     /locus_tag="ACGI8A_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFH0179643.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT        11309 a         2804 c         4875 g         9419 t
ORIGIN      
        1 aagtactaca ccttcaaata tcacctaaag taattaaaag gcaattaagg tgaagtagtt
       61 caagtatttc taaaataagt ttaataaatt ctataataat ttattttttg ccttaaaatt
      121 gccttaaaat ccaaacaatt ttaaaactaa tgtactataa aaagctggta taatttaaat
      181 atagtaaaaa ataagattta tcttatctat tgggatttaa ttatttaatc aatatattta
      241 taccatttaa tttaaataaa aagtctatta aggagtgagc ttatgagtaa gttaatttat
      301 atagcagatg atgaagataa tataagaaat ttggtaaaga cattcttaaa aaatgaaggt
      361 catgatgtta tggattttaa aacgggtgat gaacttttag aacaatttaa tataaaggag
      421 tgtgacttag taatactaga tataatgatg cctggctcaa gtggttttga agtatgtaca
      481 aaacttcgtg agaaaagtac agtcccaata atcatgctta ctgcaagaga tacagatata
      541 gattatatta cagggataac cttaggtagt gatgattatt ttacaaaacc atttagccca
      601 atgtctttgg ttatgagagt aaaatctata tttagaagga tagagtttga aaagaaacaa
      661 aattatgata aatatagtaa ctcgatagat atggaattaa aatttggtga tgtaataata
      721 aataaaaaga ataaaatcgt tacaagtaaa aatgtaaata tagaccttac accaaatgaa
      781 tataacctat tgacttattt gtttgaaaat atagatagag cagtttccag agatgaatta
      841 ttaaacaaaa tatggggata cgatatagaa gttgagacaa gggctgctga tgatacagtg
      901 aagagattac gtaaaaaaat tttaaataca aatattttaa tagaaactgt atgggggttt
      961 ggatttaggt tgaaggagaa aagttaagat gaagaatgga aatgacaaga atagaattgg
     1021 aaagattaaa tccaaattta aattaaagtt ctcaaagaaa aatataagca taaaaactag
     1081 gattttgttg agtatacttt gtttgataat tatagtattt atgataatat tagtatcttt
     1141 taatgtattt cttgatacat atataaaaac aactgcgaat gaagaattga caaagtctac
     1201 agaaatagta gaacatatgg atagtaattt tagacctatt aagccaccac aagatgatgg
     1261 aaagcttcct cccaaagtat tatctaattt tatgagaaat gtacaagata aagttaggat
     1321 ggcagaaaca caatcagatg ctgatgctat ggtagttgat tctaagtata acctgatatt
     1381 tccaacaaga gaggatgatt tcttaaaaaa tgttgatgag atggagctga ttagggagtg
     1441 tattcaaaat gaaaaattag atttaaattc agatgaaaat acacggatat ctaccagaaa
     1501 taaggattat tatatttcaa ctgtaaaaat tactagtata tatggtgaac aagacaagta
     1561 tcttatactt tttatagaca tatcaaaaac tctaaatcta gcacaaaaga taaatattgt
     1621 attaatatct gtaatgtgtt ttgctggaat attagcaata tttacagctg tgattttatc
     1681 tgagaaaatt gctaaaccga taaaagagtt gtgtgagttt gcaaaaaaaa tgggtcaagg
     1741 agatttcaaa agaacctact ttgacttttc agataaagaa ttagtagagc tctcaactgt
     1801 tatgaataag agtgctgagt atctggataa gtatgataat gaacaaaaaa tattttttca
     1861 aaatgcatct catgaattaa ggacaccact tatgtctatt aaaggttatg cagaagctat
     1921 aaaatataat gttatagata gtaaacatgc aagtgatata attcttgaag aaagtgatag
     1981 gttaagtgat atggttgaag atttattata catttctaaa atagataata taactaaaga
     2041 ttttgaactt gtagaatgtg atttaagaga agtactttca aattgtggag ctaggcaaaa
     2101 tgtaagagct ataaataagg ggataaaatt tatttatgaa tttgatgaag aaatggtttt
     2161 atttgaatgt gatgaaaaaa acatttccaa agcatttatg aatcttatag aaaatgcttt
     2221 gagatacgct aagagtgaaa taaaaatagt gtgtaagtat aatcaaaaaa atatagttgt
     2281 gataatagaa gatgatggaa ttggaatcaa gaaagaagat ttacctcatg tttttgaaag
     2341 attctataag ggagtagggg gaaatcatgg aattggatta tcaatagtta aatctattgt
     2401 aaataagcat ggtgggcgta tatacgtaga aaatggcaaa aaaggagcta aatttactat
     2461 attatttaaa ttgtaaaaaa attagagaaa atatagagat gtttgaagtt gttttttata
     2521 tatatctgtt gtatactgtt gaaaaaagaa caatattaag ctgacatata ttacgatagt
     2581 tgacatttaa agtaaatgct taaatataga tacgaaagga aaagtaaaat ctgaagtgta
     2641 tttaggttta agtaagcttc ttattttata aaaaatatgt atgaaagaac agctgatttt
     2701 taatattaca aatttagtaa cggttgtatt tgaagctttt gtcatccata tgtttttatc
     2761 agacttttta ggtcagaaag aagggtatat aaaagttgta agatatgcta aactgggttt
     2821 tataatatgt ttaggattct gtaatttaat aactttaaac cctaaaatca caatgccttt
     2881 aattttcatc cttatttttt caacatcatt tttatataaa ggaaatttaa agacacgatt
     2941 gtttactact gttttattaa gtatattctt tatactgtca gaaattgtag ttacaagtat
     3001 ttttgtttta tttgtaaaag aaggctttga aataatgctt gaaaataata gtataagagt
     3061 attggcgaca attttatcta aaattgtctt tttgttgact tgtaaaatta tttgtctatt
     3121 taaaaaggat gtacatttag atatgcccat aaagtattgg ctccctttat ttttaatacc
     3181 tatatttagt ttatttctgt ctgtttctat ctttgatgta agtaaatttt ttagtcttga
     3241 aagtttaaaa tttctaagct taatatctag tgttggtatt ttatatatta attttattgt
     3301 cttttattta tttaaattta ttatagataa aactaaacta tctatgaaat atgaactact
     3361 tgaaaaagaa ataattcata aggaagaatt aagacttagt aatgagtgtt ataaaattat
     3421 tgttgaacag actgattctg tcgttttcga atggaatatc aaagaaaata aatcatttgt
     3481 atcgcaggct tggactgaaa aatttggata taacaatgca tgtaagaata tttttaaaga
     3541 aattaaagat aaagacttag ttcattctga agataaagct atatttgaag gttttcttga
     3601 aagtattaaa aaaaagaata tgcacaatca agcagtatat cgtttaaaaa aatctaatgg
     3661 tgaatatatt tggtgtagaa catcaattac aagcatttat aatgatgaaa atgaactttt
     3721 aagagttgtc ggagtaattg tagatgtaga tagtgatata aaaaaatatg aagagttaag
     3781 aactagagca gaaagtgact cattgaccaa tatatacaat aaaggtacat ttgaaaaatt
     3841 agtggaagaa actatagtta tgaatacagg tgataaaaaa gatgcattat tcattattga
     3901 tttggatgat tttaaagaaa taaatgataa ttttggtcat ccatttggtg attttgtact
     3961 aaaaacattt gctgataaga tacaaactag ttttggtagt aaagacttag tcggaagaat
     4021 tggtggagat gaatttgttg tatatatgca agattatgtt acagaagtaa atttgcataa
     4081 aaaagctaaa gaattaaata gagtactttc ggataattac actgatttga gttttagttt
     4141 tgatgcttct gtcagtatag gtattgcaag gtatcctcaa gatggaactt cattttttga
     4201 attgtttaaa aatgcagata gagcacttta tagcataaaa gcatcaggta aaaattcata
     4261 ttgtttattt gaagaagaac tatatgtaca gtgaatttga atttagattt tatgtgagag
     4321 taagatttat atatacttgt ggctgtctga tttataaaat atgttacttt gtatattttt
     4381 atatgtttca gtaaggttaa ttaacatgag caaaggtgta agtaaattta tattagttta
     4441 agtttactta cacctttata aaaatacaaa taatagattc atttacattc ttaaatgatt
     4501 aaagctcttt accttctaaa catgcataac atatcgttat aattccagta aataagacta
     4561 aaatgcccat tactaagatt acaatttcat catctattac tccaaatgtt tttaaagtaa
     4621 cttatttcgc tttgtatcat aataatttgt ctctttttac attttaactt ctgctgagat
     4681 ttgagttaga tattcattag aatctcgcac acttatatca tatagaagat attcttcata
     4741 aacaaaatcc ccaatacgta aattattctg attaaaaaaa tttaataact tttcataagc
     4801 cttgtaaata gtttcataac tccctttatg ataagcagtt acataaagtc cctttggttt
     4861 gattgataca ggatatttct tgtactcttt attaactttt gtaaataaat atgaatatga
     4921 ttcaaagtct ttattaatta tatcttcacc tttaatgatt gagccaatcg taccataatc
     4981 actcaattca tagttgtcca taaagttaat acattcatat aaaaaatttt cttcctcatc
     5041 tcctaaaaac tgttctttat atgtaacagg tgttttgacc aagtactctt cttcatgttc
     5101 ttttaaagta atagtattcg catctatatt acatgcactt ttagtaaatg aaatagtaga
     5161 ttccattaga gcttgaatat tatttagttt ttcaatttca tttttaatat caatggtttt
     5221 cttttctaaa agctcaatta gtaaattagg tgttcttttg tctatataag ctttaatttc
     5281 ttttaaaggc atttttactt ccctaagtga agaaattata ccaaaaatgc taagttgatg
     5341 gtatgagtag tatctatatc cattttcttt ctttaattct ggtgagaaaa gacctatatc
     5401 atcataatgg aaaagtgttt ttttattgat tccacaaatc tttgcaaatt ctccagttgt
     5461 aaaataattt tttgaaactt gactcataat tataattcct ccttgactat cctgttacag
     5521 tatagtttat catagtattt tgtaaaggag tgtgattata tgaaaaaatt aattcatttt
     5581 ttaaaaccat atagagtttt aattgttgta gttttgatat ttacgttttt acaaacactt
     5641 ggaactttgt atataccaac acttacagca aatatagtaa ataatggtgt agtaaaggga
     5701 gatatagatt atattgtaaa aacaggttta atgatgatga tagtagctgg tataacagct
     5761 ttatcagctg tgttagtttg taaagtatca gcaaatcttt catctggatt ttgtagagat
     5821 attagagaag cagtatttat aaaatcacaa gatttatcaa taaatgattt taataatata
     5881 ggtacagcat caatgattac taggagtact agtgatatta ctttaatagg tcagtctgtg
     5941 tttatgttta ttcaattagt attaccagca ccaattataa ctgtttctgg tctattctta
     6001 gcatattcta tagataaagc aatgacaatt attattgttg tagtaatgtt tttattcatg
     6061 ctttcagcat ttttagttgg taaaaagctt ataaagttgt ttaaaatgat gcaaataaag
     6121 atggataata tgaatagggt gttacgtgag gtagtaactg gagtaagagt tataagagct
     6181 tttaatagga gtcgttttga aaaaaagaga tttgatagaa ctgcaataga ttattctgaa
     6241 acagcaattt caataaataa aatatttgca gtacttatgc ctattgtaat gttaataatg
     6301 aatttaggta tagtaagtat tatttggttt ggtgggatga gagtcagtaa tggaaatatg
     6361 gaaataggtc atataatggc acttgttgaa tattgtatct taattttatt ctatttaatc
     6421 atgggagtaa tggtatttat gtacatacct agagcaggtg catgtgcaga tagagtaaat
     6481 cagatattag atatagaacc tgaaattgta gatggaaatg gtcataaaga tactgtctca
     6541 gagagaggac atttagtatt taaaaatgta accttttcat atgcacaatc agaagaacct
     6601 gtgttaaata atataacatt tgaagcaaaa tctggagaag taactgcaat tataggaagc
     6661 actggctcag gcaaatctac tattgcaaat ataataccac gtttttttga aattcaatct
     6721 ggagaaatat ctataaatgg tcaggatatt aagaagattc ctcaaaaaga acttagagat
     6781 aagattggat ttgtaccaca aaaagctttt ttatttagtg gaacaataga agaaaatata
     6841 aggtatggaa aagaagatgc aagtattgaa gaagtaaagc atgctgcaag tatagctcaa
     6901 gctgatgaat ttatttatga tatggaagac aagtatgact catttgtagc acaaggtgga
     6961 aataatttgt caggtggtca aaaacagaga atatccattg caagagcgtt agttagaaag
     7021 cctgaagtat atgtctttga tgatagtttt tctgcattag attttaaaac agataaaaga
     7081 cttagaaaag cgttgaaaaa tgaaatcaaa gattcatcag ctataattat agcacaacgt
     7141 ataagtacaa tcatggatgc taatcagatt atagttttaa atgatggaaa aattgtaggt
     7201 attggaaaac ataaagattt acttgaaaat tgtgaagttt ataagcaaat tgcggattca
     7261 caacttagta aagaagaact tgcataaaat gaagaattaa tctgatattt ggaggtgtat
     7321 gatatgagtg aaaaaagaat agatgaacaa gatatagaag tacttagttt tgaagatggc
     7381 atgtcagcag aaaaagctaa ggatgcaaaa aaaactacta agagattatt aaaatatatg
     7441 gcaaaacaaa agtttaaaat attaattatt tttatttcag tattgataag ttcagcgctt
     7501 acagttttag cacctatggt tatgggaaaa gctattgacc aactatttaa tggaataaaa
     7561 actgcagtcc aaacaggtac aaaattcagt gtgaatttta gtactatggg agggatagta
     7621 tcaatacttc tagggttgta cttaattagt gcagtattta tatatattca acagtatatt
     7681 atgtcaggag ttgcacaaaa tctagttctt agcatgagaa aagatttaag tgataaactc
     7741 aataaacttc ctcttaagta ctatgattct cataaaaagg gggaaacact tagtatagta
     7801 acaaatgatt tagaaaaagt agcagatagt ttacaagaag gattaatgca acttattaca
     7861 gcagtagtta cagtagttgg ttctattgta atgatgataa gtataagtgt accacttaca
     7921 atagtatcag ctataacatt acttgtttca ttgggtatta ctgttgtaat tgcaagaaaa
     7981 tcacaagaaa gattctctga aaatcaaaag gcactaggag agttaaatag caatatagaa
     8041 gagatattta ctggtcaaat agtggttaaa gcatttagta aagaaaagga tactataaga
     8101 aactttaaaa tattaaatca aaatctttat aatgcaagta gaaaagcaca gttttcgtca
     8161 tatgcaatat ctccaattat tagatttata aacctaattg gctatgttat tatagcagtt
     8221 gtaggtggga tatttgcatc aactggagca atgactcttg gtagtataca agcgtttatt
     8281 caatatgtaa atcaagcatc agagcctact acagaaatat cttatattgt aaatatgctt
     8341 caagcagcaa tagcatctgc agagagagtt tttacagtta tggacgaggt ggaagagata
     8401 aaggatgaag aatcttcaaa ggttattagc atgcctaaag gtaaagtaca atttgagcat
     8461 gttaaatttg gatatagtga tgattttatt cttatgaaag atattaatat caatctaaat
     8521 gcaggagata agattgcaat tgttggacct acaggagctg gaaagacaac tcttgtaaat
     8581 ttacttatga gattttatga gatacaaggt ggaagaatta ctattgatgg tgttaatata
     8641 aaagatttaa agcgtggaga acttagaact atgtttggta tggttcttca agatacatgg
     8701 ctgtttaatg gaagtataaa agaaaatatt gcatatagta agtcagatgc cacaatggat
     8761 gaaattgtaa gagcagcaaa atcagcaaga gtagaccatt ttataaggac tttaccacaa
     8821 gggtatgaca ctatattgac tgaggatgct tctaatctat ctcaaggtca aaagcaactt
     8881 ctaacaattg ccagggcaat actttcagac ccatcagtat tgattcttga tgaagcaaca
     8941 tctagtgtag atactagaac agaggtagaa attcaaaagg caatgaataa cttgatgaag
     9001 ggaagaacta gttttgttat tgcacataga ttatcaacta ttcgagatgc tgatttaata
     9061 ttagtaatga aagaaggaac tattattgaa caaggaagcc ataaggaact tattgaaaag
     9121 aaaggattct atgaagaact ttataacagt caatttacaa gtgaatatga tgaagatgtt
     9181 gtatgattat caagttttag gcaactaaat gattgataaa tattaagtta aatatagtaa
     9241 catgtaaggt tatatggaat aagagtattt ctctatttca tataaccttt tttatttatc
     9301 aaaataagat aaaatttcct ttctaaatta gattgaagaa tgtatttatc aatataaaag
     9361 taaaataatc attattatta gtgaagtaaa tattgtttgc cacatatatg ccataaagat
     9421 gcacttattt tacggagatt taacaaagta gtgtaaagta tactctaaat ataaaataaa
     9481 tcaaatagag ttaaataaat tgagaggtga tataaatagt gaataaaaaa ttgaactata
     9541 gatttgaaat gaagcacaaa ataactgaag cagatgtgtt agctttaaaa tcaagactct
     9601 atccaataat gaagaaagat gaaaatgcat ctaaagatgg aaaatatctt attaggagtt
     9661 tatattttga tactcctgaa gataaagcac ttttggataa attaaatggc gttgcaataa
     9721 gagaaaagtt taggataaga ttttataata atgactgctc ttatataagg ttagagaaaa
     9781 aaatcaaaca ttacaacatg acatcaaagc tatcaactaa tttaaccaaa aaagaagtaa
     9841 taaatatact aaataatgat attgaatttc taaaagagtc gacaaattgt ttattaagag
     9901 aattttattt gaaattaaaa tgtgaaagat tagaggcaaa aagcatagtt gattacaata
     9961 gagaagcttt tatttatcca gttggaaatg taagagttac aatcgatagt gatattaaaa
    10021 cttctgtaaa ttcggtagat ttatttaata aagatttgcc aactatttcg gtaattgatg
    10081 agaatatgac tgtattggag gttaaatatg atgaatttat accagatttt ataaaggatt
    10141 taattcaaat taataagact acctctacag cagtttcaaa gtatgcttca agtagattat
    10201 atatttaaga ataaaggcta tgatttatat aaagatatac ataaataagg ttaaaaaaca
    10261 aataagttaa aaataaataa aataaaaaat aaataagtta aaaataggaa ggatgatttt
    10321 aattatgaca tttaatgata tttttaaatc aagttttata gaaaacgtat caggtttttc
    10381 atttgtggac agtgctttag cactaggttc agctttttta gtaggtttgt ttatatatat
    10441 ggtctataaa aagacttata tgggaattat gtattctcga ccattcaatg tgtcgcttgt
    10501 agcacttaca atgcttacaa cttttgtaat attggcggtt acttcaaatg tagttttatc
    10561 cttaggtatg gttggtgctt tatctatagt aagatttaga acagcaatta aagacccaat
    10621 ggatttagta tttctatttt ggtcattagg ttcaggtata gttttaggag caggtttgat
    10681 tcccttagct gtcatgggtt caataattat gggattgatt ctaatattct tttcaaataa
    10741 aacaatatct gaaactccat atatattgat ggtaaattgt aataatgaag attcagaaga
    10801 tatagcaact gataaaatta aaaaagtatt tactaaatat caaataaaat caaaaagtgt
    10861 tacaccagaa aaaggaatag aattagtgtt tgaagtaaga atgaaagatg gagaaacaag
    10921 tcttataaat gacttgagtc aagtagatgg agttactaat gcagttcttg taagttataa
    10981 tggtgactat gtagcataga gaggagtaat cttttatgaa agataaaaaa tttacccttc
    11041 ttatctcgat tatgattata tttttatgtg ctgtagttgg agtttatagt acatctagca
    11101 acaaaagtgc tgatttatat agtgatgtat atattgaaaa atattttaac agagacaagg
    11161 ttatggaagt taatatagag atagatgaaa gtgacttgaa ggatatgaat gaaaatgcta
    11221 taaaagaaga atttaaggtt gcaaaagtaa ctgtagatgg agatacatat ggaaacgtag
    11281 gtataagaac taaaggaaat tcaagtctta tatctgtagc aaatagtgat agtgatagat
    11341 acagctataa gattaatttt gataagtata atactagtca aagtatggaa gggcttactc
    11401 aattaaatct taataactgt tactctgacc catcttatat gagagagttt ttaacatata
    11461 gtatttgcga ggaaatggga ttagcgactc cagaatttgc atatgctaaa gtctctataa
    11521 atggcgaata tcatggtttg tatttggcag tagaaggatt aaaagagtct tatcttgaaa
    11581 ataattttgg taatgtaact ggagacttat ataagtcaga tgaaggaagc tcgttgcaat
    11641 ataaaggaga tgacccagaa agttactcaa acttaatcgt tgaaagtgat aaaaagacag
    11701 ctgattggtc taaaatcaca aaactattaa aatctttgga tacaggtgaa gatattgaaa
    11761 aatatcttga tgtagattct gtccttaaaa atatagcaat aaatacagct ttattaaacc
    11821 ttgatagcta tcaagggagt tttgcccata actattattt atatgagcaa gatggagtat
    11881 tttctatgtt accatgggat tttaatatgt catttggtgg atttagtggt tttggtggag
    11941 gtagtcaatc tatagcaatt gatgaaccta cgacaggtaa tttagaagac agacctctca
    12001 tatcctcgtt attaaaaaat gagacataca aaacaaaata ccataaatat ctggaagaga
    12061 tagtaacaaa atacctagat tcagactatt tagagaatat gacaacaaaa ttgcatgaca
    12121 tgatagcatc atatgtaaaa gaagacccaa cagcatttta tacttatgaa gaatttgaaa
    12181 aaaatataac atcttcaatt gaagattcta gtgataataa gggatttggt aataaagggt
    12241 ttgacaacaa taactctaat aacagtgatt ctaataataa ttctaatagt gaaaataagc
    12301 gctctggaaa tcaaagtgat gaaaaagaag ttaatgctga attaacatca agcgtagtca
    12361 aagctaatac agataatgaa actaaaaata aaactacaaa tgatagtgaa agtaagaata
    12421 atacagataa agataaaagt ggaaatgata ataatcaaaa gctagaaggt cctatgggta
    12481 aaggaggtaa gtcaatacca ggggttttgg aagttgcaga agatatgagt aaaactataa
    12541 aatctcaatt aagtggagaa acttcttcga caaagcaaaa ctctggtgat gaaagttcaa
    12601 gtggaattaa aggtagtgaa aagtttgatg aggatatgag tggtatgcca gaaccacctg
    12661 agggaatgga tggtaaaatg ccaccaggaa tgggtaatat ggataaggga gatatgaatg
    12721 gtaaaaatgg caatatgaat atggatagaa atcaagataa tccaagagaa gctggaggtt
    12781 ttggcaatag aggaggaggc tctgtgagta aaacaacaac atacttcaaa ttaattttag
    12841 gtggagcttc aatgataata atgtcgatta tgttagttgg tgtatcaagg gtaaagagaa
    12901 gaagatttat aaagtcaaaa taagattaaa attgaatata aataatttta atactgttaa
    12961 gtctaaatat ttacttagca gtattttttt agaaaataat gtatttggtt aataatataa
    13021 ggtaataaat tgataaaaat taaatacagc atattaataa tacataataa agtaataaaa
    13081 aaactgttta agtataataa cttatattta gacagttttt ttcatttata aataatataa
    13141 atgaaattga aaacatttga aggatagaaa ttggttggat aaatgtataa attacttcac
    13201 caaaaaataa tgacaaaaat tgtaactaag ttgtaataaa atggtagtta tttagcataa
    13261 aataatgtat catagaagta tattataaaa ttaataaaat aagatttttg taagaaaact
    13321 gctggctttt gatatttaga gtgagtagtt aaattatgtt taggaggggt agaatttgtt
    13381 gcaaaaagta ttatggaaat ttttaaagct agtagtcggt ttatttatat gttcagttgg
    13441 tatagtattg acgataaatt gtaaccttgg attatctccc tgggatgtat ttcatcaagg
    13501 actttcaaat catataggaa taactatagg aactgcttct ataattgtag gctctatagt
    13561 agttattgct gatgtcgttc ttggagaaaa tgtaggatgg ggaactgttt ttaacatgct
    13621 actaattgga ttctttatgg atttactttt atatagtaat ttaattccag aggctgatag
    13681 tttatttgta ggaataatta tgttgatatt aggattggtt cttttgtctg ttggaatggt
    13741 attttatatg ggaagtggtt taggaagtgg acctagagat ggtttgatgg ttgcaattca
    13801 aaagaaaaca ggaaaatcac ttaaattaat aagaggtact atagaagttg gagcattaat
    13861 agtaggattt ttattaggtg gaaaagttgg tataggaact ataataagtg catttggatt
    13921 ggggtatttc acacaaatgg tatttggctt atttaaatta gattgtagta agattaaaca
    13981 tagatttata gtagatgata taaaatttat caaagtgtat atgagtggtg agaaaaaagc
    14041 tgctgttcaa aatatagtta agtaatttaa gaataaattc aacgaaatgt taagtatttt
    14101 aaattaacat tttgtttaga tagataaatt gaatattaaa agaatattaa aatatgagga
    14161 ttgctagaac actataaaat atagatttct agcaattttt tatttgttca atattttttt
    14221 agtactagaa gtcttattct ctatttaatg aatgaattaa tttataatta gagatactaa
    14281 actttataga tattaatata aacaccttta aactgtgtat tttccaaagc ctttaaaatg
    14341 cttgtattta ttctaaataa aaaaataaaa aaatgtcaaa aaaactcttg tattatatcc
    14401 aaattttata tataattacc tatgttgttt agaaatttta aactcaatgt ttagtaaaca
    14461 aaatgtttag taaaataaaa cagaaactag gaggtgcaac atggatatag gtgaaaagat
    14521 aaaaagatta agaacagaaa aacaactgac ccaggaagag ttagctaata gatgtgaatt
    14581 gtcaaaggga tttatttctc aattagaaaa taacttaaca tcaccatcca tagctaccct
    14641 tatagatata cttgaaatac taggtacaaa tttaagggaa ttttttaatg aaatagatga
    14701 tgaaagaatc agttttacaa aagaagatat gtttgaaaca gaagatgaag atttaaaata
    14761 taaattaaag tggttaattc caaattcaca aaaaaatgaa atggagccaa taataataac
    14821 attgtatcca ggaggacagt ataaagaaga aaagccacat gaaggtgaag aatttggata
    14881 tgtattagca ggttcaatat atgtccatat aggcgaaaag aaaaataaag taaaaaaagg
    14941 agagagtttt tactttagac cgaaagcaaa tcactatata tcaaacgagg gcaaaactac
    15001 agcaaaagtg atttgggtaa gtacgccacc atcattttag aatagaggtg tttaataatt
    15061 ggtagaaaat ataatagaat taagggatat atctaaacat tatgaagaac ttactgtttt
    15121 agataatttc aatttagata taaagaaaaa tgagtttcta acattacttg gaccaagtgg
    15181 atgtggaaaa actacaacat taaaaataat agcaggattt gagtatgctg atgatggtaa
    15241 agttttattt gaaggtaagg agataaataa tttgccacca tatgaaagac aagtaaacac
    15301 agtatttcaa aaatatgctt tatttccaca catggatgtt tatgaaaatg tagcttttgg
    15361 attaaaaatt aaaaagactc caaaagatgt aatagatgct aaagtaaaag aggttttgaa
    15421 acttgttgca ctagaaggtt ttgaaagacg acaagttgaa tcattgagtg gaggacaaca
    15481 acaaagaatt gcaatagcaa gagctctagt taatgaacca aaggtattat tactagatga
    15541 accacttgga gcacttgatt taaagttaag acaagagatg caaatagaat taaagagaat
    15601 ccaaaaaaaa ttaggaataa catttgtttt tgtaactcat gaccaagaag aggctcttac
    15661 tatgtcagat actattgttg taatgtataa gggcaaaatt caacaaatgg gaacaccaca
    15721 agatatatac aatgaaccta aaaattcatt tgtagctaag tttataggtg aaagtaacat
    15781 atttgatgga attatgatag aagattataa agttaatttc tgtaatagag attttgagtg
    15841 tgttgataaa ggattcgaaa aaaacgaaaa tatagaagta gtaataagac cagaagatat
    15901 aaaaatggta aagccagaag aaggaatgtt aaaggggaaa gttacatcta cagtgtttaa
    15961 aggagtgcat tacgagatag aattgaatga aaatggaaga ttgtggatac ttcataatac
    16021 taaaaatgct gaggttggga ctgaactagg catggatata tatccagaag atatccatat
    16081 aatgagaaaa gagaataact aagatgaaaa gaaaatcttt tttagcatat ccatatgtag
    16141 tttggagtgc gatatttgta ataattcctt taatattagt agtatttttt agttttacaa
    16201 aagaatcagg tggaggttat gcctttacac ttgagaatta taaagaagta attgacccta
    16261 tatatatgaa agtttttggg cgttctattc ttttagcagg aggagctaca ttaatatgcc
    16321 ttatagttgg ttatccagtg gcatatataa tttcaaaggc tcgtgtaagt agaagaggtt
    16381 cattaatatt attgtttatt cttcctatgt ggatgaactt tcttttaaga acatatgcat
    16441 gggttgctat attaggaaaa aatggacttt taaatacatt tttaggttgg tttggaatac
    16501 aaccattagc aattttgtat actaattttg ccatactttt aggtatggtg tataactttt
    16561 taccatttat ggttcttcca atatatacag cactatcaaa gatggataat gatttgataa
    16621 acgctgctca tgacttgggt gcaaataata tgacagtttt tagaaaaatt atatttcctt
    16681 tgagtttgcc aggtgtaatg tctggaataa ctatggtatt tatgccagca gtcactactt
    16741 ttgcaatatc aagactgtta ggtggaggta aaataatgct ggttggagat ttgattgaac
    16801 aacagtttac agttgttgga gattggaact ttggttcagc tatatcgata tttatgatga
    16861 tagtaatatt aatatctatg tctataatgt caaaatttgg agatgaatct gacaaagaag
    16921 gcggtgggtt actattttaa aattaaagaa aataacttct aatatatatt tagcattagt
    16981 attcttattt ttgtatgcac ccatatttgc tttggtacta ttttctttca atgattcaaa
    17041 atcaatggca agatggaatg gatttacatg gaagtggtat ggtcaattac ttcaaaatga
    17101 gagtataatg agtgctttat attatactat tgtaatagct atattagcgt ctgtaatatc
    17161 tacaatagta ggaactataa gtgctatagg aatacataag atgagaggta agagtaagaa
    17221 attaatacta aatgtaaact atcttcctat tttaaataca gagatagtta cagcagtagc
    17281 tcttatgagt ctgtttgtat ttgtaaaaat ggaatttgga tttacaacaa tgctattggc
    17341 acatataatg ttttgtttgc catacgttat actttcagtt ttgccaaaaa tgaagcaatt
    17401 acctgataat atagaagatg cagctatgga cttgggagca acacctatat acgctcttag
    17461 aaaagtaatt ttaccacaaa taaaaccagg tatagtttct ggatttttaa ttgcatttac
    17521 tatgtcaata gatgacttta taataagttt ctttaatgct ggaaatggtg taagcaatct
    17581 ttctatagaa atctatggaa tggctagaag aggtataaaa cctgaaataa atgcattatc
    17641 aacaataatg tttgcagtgg ttctaggttt acttttacta gctaataaaa aagaatctat
    17701 agtaaggggg attaaataat gattaagttt aaaaaagcaa tatcccttat agctgtaggt
    17761 atgatgacag ttactttatt tacaggatgt aataaagcaa ctgattctac aaaagtttta
    17821 aatgtatata atgttggaga ttatatagat gagtctctta tagataagtt tgaaaaagaa
    17881 acaggaatag atgttcaata ttcaacttat gatactaatg aaatgatgta tcaaaaagta
    17941 aagagtggta gtacacacta tgatttagta tttccttcag actatatggt agagaaaatg
    18001 aaaaatgaag gtcttttgga aaaattagat tttaagaata tacctaatat gaaatattta
    18061 gataagagtt ttttaaatcc tgtatacgac aaaactaatg aatatagtgt tgcatatatg
    18121 tggggaactc ttggaatact gtacaataag aaagaagtta aagaccctat ggatagctgg
    18181 aatatacttt ggaatccaaa gtataaggga aatataatga tgtttgactc tgttagagat
    18241 actataggga taactcttaa aaaattagga tatagcatga atagtgtaaa tccaaaagaa
    18301 ataaatgaag ctaaaaatct tttaatgaaa caaaaggatt tagtattggc ttatgtaaat
    18361 gatgaaggta aggatagatt gcttggtgga gaagtggcaa tgggtatgct gtattctgga
    18421 gatgcagtta cactaatgga gcaaaatcct gaccttgatt atgctatacc taaagaggga
    18481 actaataaat gggtagatgc tatgtgtgta cctacaacag cacaaaataa gaaagaagct
    18541 gagctgttta taaacttttt attagaccca gagaatgcaa aagtaaatgc tgagtacatt
    18601 gggtattcaa cacctaatac aggagcacta aaattattag acccagaaat aactcagaat
    18661 cctgttgcat atccatctaa agaagtgctt gataaatgtg aaacttttga agatattggt
    18721 gaaaatataa aattatatga caaggcttgg atagagttaa aatcaaaata gggtcttgtt
    18781 ggtttaagta aatttactta ataaatactt aataaaactg ttctttttta gaaaatttat
    18841 tttctaatta ttgaacagtt ttattttttg tttaagttat attcatatgc aatttaaata
    18901 gtttattaat aaatgaaaac ttatttgtat aatgacttta ataataatta gaaaaaatgg
    18961 gcaacttaat agaataattg gaggtgtaaa tttataaaaa atgaatagga taaataaaaa
    19021 aatagattta ttaatattgg ctttgctcgt ttttatattt ttaattgttg gaatagtaat
    19081 actatcgatt aggacagaaa ataaattgaa tgtgttcata atgttagcaa taattttttt
    19141 tataataatg ttaacttatt tatcaaattc agtagtgggg ttgataacaa gttcaataat
    19201 tatatttatg tatacatcct atattttata taacaatatt actcacaata tggatgttga
    19261 atttatatca tatatgtgga taatagcaac accagtatct tcaattataa tgggtaatct
    19321 gaacaaaagc ataaatgaat tgcaaaatac aaataaaaaa ctatctgaac agtataagga
    19381 attagtgact attgatagtg aaactggact tagaaattta aagatttttt ataatgatgt
    19441 aaatatggag ataagtaaat ctattagaca taatactgat ttttctctta tgatagtaaa
    19501 attaccatac tacggaaacc tacagactat atttggtgaa aacaaaacta ataaaatagt
    19561 aaaatatatt ggttcaaata ttatagaatg tacaagaaat gaagatataa tttatagttt
    19621 acaaaaagat atgataggga tactaatgcc aaatacatct ttggaaggtt caaaagttgt
    19681 taaagacaga ataaagaaaa ggataaaaga attaaattta gatttaaaca ataggggaaa
    19741 atatgtaaat atagatgtca aaattgcatt tttacaatat aagagtagtt ttggagatag
    19801 tataaatttt aagaatatag ttgaagagga acttcaatat gatgtttaaa atatttaata
    19861 aaagtctatt aaaattagta gttgtattat caatagtact gacaatatca aattttagct
    19921 tatcttcata tgcatcagaa attacttcca gcacagataa ggtattaatc atatatgatt
    19981 ctaagaaaga aacagcttat aatagagata ttttaaatat tatgaggact ctattgggaa
    20041 gatttagttc agatatagag ctattaaaat tgagtaacta tgatggtgag ataaataaaa
    20101 attattacag tcatatattt attttaggaa tcaatgaaaa ctcatataat aatgataaaa
    20161 acactaaaaa cttgattagt tccttaaatt tatataaagg aactatatgt tggttaggat
    20221 atggtataga aaatcttttg gaacataaaa aatacaattt agactatgta ggaaaaacaa
    20281 acaatatagt tagtgtaaat tatagaggta aatcatataa cttggatgaa cattatgttt
    20341 ttaacattgt agaatcaaaa gatacaagta ataaagtaat aggaagtatt aatgatacct
    20401 taaataagta tccatatata ataaatgata aaaatctttt ttatgtatca aaattagatt
    20461 tagatggagt tttattttat atattttgcg attcacttaa tgatattttt aatattaaga
    20521 cgtttgataa aggacgtata tttgtaagaa tagaagatgt acatgctttt agagaaccaa
    20581 aaaatcttgt agagatagcg gattatttaa gtagtaagaa tataccattt acgattgcat
    20641 taataccagc atatgtaaat cctaaaaatc ataaggtaat aactttatca gaaagccctg
    20701 aaattgtcaa agcaataaaa tatatgcaag ataaaggtgg aactgtgata ttgcatggat
    20761 atactcatca atacaagaaa gaagaagttt ctggagaagg gtatgaattt tgggatggta
    20821 aaaaagatga acccttaaaa gaaaatatga agatatttgt caaagataga gttttgaatg
    20881 gtcttagagt atgtatagag aatggaattt atcctcttgc atttgaagcg ccacattatg
    20941 caatggaatc agagggatat aaagaattaa aaaaatattt ttctacttat atagggcaac
    21001 atcaaaataa tgataaaaaa tttagcacaa atacatatcc ttatattatt agggatacag
    21061 aggagtttaa tatatttata cccgaaaatt taggatatat agacccagaa gataagttta
    21121 cttttcaaaa tataaaagaa aatttagata aattatccat tgtaagaggt tttagtggag
    21181 ggttcttctt tcattcatat ttaaacatag agtatctaaa aaacacaata gaatatttag
    21241 aaaaacagaa tatagaattt atgaatttaa gagattttaa taattgggta aaagttgatg
    21301 aaatacaaat aagaaataat ggagatgaaa ttatagtcaa ctatgataag gacttagatg
    21361 aactaactaa aagtgatact agatttaaat ccatatccaa tattagtaaa gttttaatat
    21421 tcatagtatc tataagtgtt ttaatatttg tgattatatt tatatatttt aagagaatag
    21481 ataaaaagaa atttttaaaa taaaggattt tatattagaa tcaatatttt aaaatatttt
    21541 aggagaaatt tatgagagat ttaaatgtat cagagtattt gttcgtattt agtttgttta
    21601 gtatatggtc tttattgctt ataaatatta ttttagctat gggtggatat atattttatt
    21661 ttaaaaactt tgataaagaa ataaaagaaa ttgatgaata tcctatgata tcaatacttg
    21721 ttcctgctca taatgaagct aaagtgatag gtagaactgt ggagtctttg ctattactca
    21781 attatcctaa gagtaagatg gaacttattg ttataaatga caattcttct gataatagta
    21841 aagaaatttt agaaaatata aaggataggt acaacaatta taatttcact ataataaata
    21901 cagatagttt aacaggtgga aaaggaaagt ctaatgcatt aaatattgga tatacgattt
    21961 caaaaggaga ttttatagct gtttatgatg cagataacac accagataaa aatgcactta
    22021 gatatctagt acaaactata gtaatgaatg atgaattagg tgctgttatt gggaaattta
    22081 ggactcgaaa taaaaataaa aacctattaa ccaaatttat aaatatagaa acattaagct
    22141 ttcaatggat gtcacaagct ggtagatggc aactttttaa tctatgtaca ataccaggaa
    22201 ctaactttat attgaggaga agtatcatag aagaaattgg aggatgggat agtaaagcaa
    22261 ttgcagaaga tacagaaata agttttagga tatataaatt gggatacaag ataaagcttg
    22321 taccacaatc tataacatgg gaacaagagc cagaaactgt taaggtgtgg ataaagcaac
    22381 gtactagatg ggcaaaaggg aatatatatg tattaatgaa atatataaaa aatatattta
    22441 aacagggaag aaataagatt gtgtttgata tagcttactt tttttctgta tattttctat
    22501 ttttaacatc tgtaattata tccgatattt tatttgtttt aagtatatct aaactagtag
    22561 aaatttctat tccaattaac ttttttctaa tatggatact atcttatctt ttatttatta
    22621 tagaagtgag tataagcctt actatagaaa aaggtgaagc aacaattgaa aatatattca
    22681 tagtggcaat aatgtatttt acatactctc aactatggct gtttgtagct ataaaaggaa
    22741 tgatagagta tctaaaagat ataattttta aaagagaggt taaatggtat aaaacagaaa
    22801 gattttgatt catgtacagg aggaattaac atgaaaaaat ttattatatc aattatatct
    22861 ttagtattgt ttttttcaaa tatatcactt atatacaaag taaatgctga tgagactaaa
    22921 gttaaaaatt ataagtttga aagagatata actattgatg gtgttatagg aagtaatagt
    22981 actttttttg aagtaaataa aaattgggat attgaagaag ttttattgca tcttaatttt
    23041 agtaagagcc aaatattaaa tggagatgtt tcaagtttaa ctgtattgat aaataatgta
    23101 ccaataaaat ctattaaatt aaatgctaag acaaattata aaaatacatt agaagtatta
    23161 gttccaaaag attatataat tcaaggatat aatgaaataa aaataaaaac atacaaaaca
    23221 atatctgata aaatttgcca agatgattcc aatactggga actggatggt tattcacaaa
    23281 gaatcatata tatcaattag atataagcag aaaaaggtgg aaaactcaat aaatgaatat
    23341 ccgtatccat atgcagaaat tgaaaataat cataaattag atacaactat agtagtacca
    23401 gataatatga ctagaggaga gactacagca gtgtttaatt tggcatcagc atttggaaaa
    23461 ataacaaaaa atgatgattt aaagttagat gttaaattat attcagaaat gaaaaattgg
    23521 tcagatgata atattatata tattggtaaa cctgaaaata cagcagaaga aattttggat
    23581 attttaagta taaaggaaca aaccctatta tcttcaaact gtataatcaa gcaagtagat
    23641 tcaccataca ataagaataa gaagatgatg gtggtaattg gtagtaatga agatgattta
    23701 ataaaggcat caaatttatt aatagaaaat agactttcta accaagtttt atcatcatca
    23761 gtacttgtaa acaaagagac taatataaaa attaataggg aacaaaaatt aaatttaggt
    23821 catttaacat taaaagattt aggatattct gactttttgt tggaaggagc atttaatcaa
    23881 caagctttgt ttgatgtcaa aataccaacg ggtaaggtat tagatgatgg ttcaaagatt
    23941 atcttaaatt taagatattc tgacaattta gattttgaaa aatctttagt tacagtgtct
    24001 ataaatgatg ttattgtggg aagtaaaaaa ttggataggt cacattctaa taatgataag
    24061 ttagaattaa aaatacctaa agatatagat aataaaaact attatcaagt taaattgaca
    24121 tttaatttga gtataaaaaa ttcgaattgt gttactagag agagtaataa tccttgggca
    24181 tatgtatcaa ataattctta tttggcgtta tctacgaaag aaaatgaaac cttatctttt
    24241 gaaaattatc cgtatccatt tgttagagat gatgaattta atgatttaac tgtgataatg
    24301 ccagattatt cagggtcaca agctatgacc tggatgttta gattaggtgt tactttaggt
    24361 gcgaatataa attcacataa tggaaatata aatgtaatta gaggaaaaga atttagtgac
    24421 aagtataagg atactaatat tgtagttttt ggagtgccac ataataattc tgttataaag
    24481 atgttaaata ataatttaaa tataaagttc gataaaaatt actccaattt tatatcaaat
    24541 gataagataa gctttattga tgattatgga aaaaatattt ctactattca attaataaaa
    24601 tcaccatata ataatcaaaa aaatatcatg gtaattagtt caatgaatga aaaaaactta
    24661 tatttaggaa tggattattt gttgaataaa agtaaagtta atgatttaaa aggagataca
    24721 ttgataattg atgaatacgg agaggtagaa gatttagctt ataatttaaa atctaagaaa
    24781 gaagtaaaag attcaagttg gaatatgagt ataaataaga caactaaagt atttttaatg
    24841 atttctttta ttactataat tatagtcatg atattatcaa tgctatatat aaaaaaatat
    24901 aaaagaagat aatatgataa atttattatt ttaaaagagg tatataattt gaggaaatat
    24961 atgaatgaat ttaaattaaa aagaagtatt ttaatctcat taataatctt aatttttata
    25021 tttatagttt actttgtaag tattgagtta aaagatagga gtctagtaaa taatcaatat
    25081 aaaggtaaga ttaaatcggc caatttatct gtagattatg aaataaatca agttatgaag
    25141 gatatagata aattgggatt gaatacagta aatgtaccta taattataaa tgtagagtcc
    25201 attaattctg atattatgtc aatagataat aatagtaaag aaaaggcaat taagttaata
    25261 aaaaaattaa ataaaaaagg tatttcaaca atattagagg catatccatg gattgaaaat
    25321 ggaaaattgt atgagactga ctggaaccct ataaataaaa aaagattttt ttatacttgg
    25381 caaaatgcca tattaaatga acttataatt gatgtagcaa atccactgga tgtaaatgtt
    25441 ttaaatattg gttctaattt tgtacatttg gaagaatatc aacaaaattg gggtgaaata
    25501 atagattttg tacaatccaa atttgacggt cttgtaacgt acagaactaa ttggtggtat
    25561 acaaaaaaag atgatttaag tagtaagttg ttttatgaac aaaagctgaa caataatttt
    25621 tttgataagt tagattttat atctatagca gcatattttg agttaagtaa taaacctgtt
    25681 aatacagtgg atgaattaat ttctgcctta cactcatcta ctgtaaacaa tagaggacaa
    25741 aatataaaac aagaaatata taacctttat aaagctcata gaaaacctat attttttgga
    25801 gaattaggtt tttcaaatag agaaagtgca agttctcagc cgtggaatca tactccttca
    25861 aaagctgtaa atggcgaaga acaagcaagg tgttttgagg catataagaa agtttttgaa
    25921 aatgaagact ggataaatgg tttttctgtg ttttgtgtag gtaaaataga tgacgaaaaa
    25981 aatttttacc catcaaaaga gagtataaaa gttattaagt cttggtatga ataattttga
    26041 aagtgtagaa tttatataga ttaaatagat gattaggaga gatataaatg aatgacatca
    26101 aggttatgac tgtatttgga actagacctg aagctatcaa agtagcacct ttaataaagg
    26161 aacttgaaaa aagagagaat ataaaaagta ttgtttgcgt tacagctcaa catagagaaa
    26221 tgcttgacca agttatagaa acctttaata taaatgtaga ctatgatttg gatataatgg
    26281 aaaaaggaca aagcctgaat gatataacat gtaagatttt aaataaactt cccttgattt
    26341 taaacaagga aaatccaaat ataatattgg tacatggaga tacaaccaca actctagcta
    26401 caagtttgac tgcattttat aataaaactc ttgttggtca tatagaagct gggcttagaa
    26461 cctatgataa atattctcct tttccagagg agctaaatag acaacttaca ggtattattg
    26521 ctgatatgca ctttgctcct actaatttgg ctagaaaaaa cttgatttct gaaggaaaac
    26581 ctaataataa tatatttgta actggtaata ctgctattga tgctttaaag atgacaataa
    26641 aagaaaacta taatcatcct ataattgatg agattggtaa cgataggatg attttgttaa
    26701 cctctcacag aagagaaaat ttgggtaaac ctatgaaaaa tatattcaga gctataaagc
    26761 gaatagtaga tgactttgaa gatgtacaga tagtatatcc catacattta aatcctaaaa
    26821 tacgaactat agctgatgag atatttggaa aatttcctga aaaaattcat attatagagc
    26881 ctttagatgt agctgatttt cataactttt taaataaatc ttatatgata atgactgata
    26941 gtggaggaat acaggaagaa gctccatcat taggtaagcc agttcttgtg ttgcgtgata
    27001 aaacagaaag aacagaagga attgaagcaa agacacttaa attagtaggt actaatgaag
    27061 atagaatata taattcagtg agcgatttat taataaataa agataactat gttcagatga
    27121 gtaaagcatc taatccatat ggagatggaa atgcaagcaa atatatagtg gatataataa
    27181 ttaaaaaatt taattgcaaa tatttaaatt agataaggcc taaaacatta actaattgat
    27241 ataagattta tgataatatt aaggaataca atttttaagg gggagtaata tggctagaaa
    27301 attaataaaa aatttgggta aaagtaaaag tgtaaagaga gtgaaacttt tatttaagaa
    27361 gatttttatt actgtattca tagtagcaag tatagttgct atttttaata taacgaaata
    27421 ttttgaggaa ttatacaaag taagggattt aaaaagtact aaaattgaat actatatgga
    27481 tgtggctgat gaagcaggag atggaaaggt ccaattaagt tggaaggcct tacttgctat
    27541 agacatggta atacatgatg aagatttaag taatataaaa aagaaagaca cattggatat
    27601 aggggaaaag tttatagtag aagataaaaa tggtaaaggc gaaaaggtgt ataaggtaaa
    27661 aaagtttaat aaggtattaa gcgaattgaa atttgactct tctcaaaaaa gtagagcaag
    27721 aaaatacatg aaagatttag aatacacata ccttggaaat aaacaattag atagtagtga
    27781 tgaaaaaatt aaatttataa agaagttaga agactcagct ataagagaat atattgatta
    27841 tggaatattg ccctctataa caattggaca agctatatta gaatctggtt ggggaaattc
    27901 taaacttaca aaacagagta ataatttatt tggtataaaa gcagataaag catggaaagg
    27961 aaaaagtgta gaaatttcaa cttcagagca ttataatgaa aaaattgtag ctagttttag
    28021 gtcgtataat tcattacaag attctgtcaa agaccacagt ttatttttaa ttaataataa
    28081 aagatataga aaacatgggt tgtttgaggc aaaagattat attagtcaag ctcaagcatt
    28141 agaaaatgca ggatatagta cagcagaaga taaaaaagga aatcgtatat atgcagagct
    28201 attaatagat gttattagga gttataattt gcaattgata gataataaag ttgagacaaa
    28261 ataattgtta actttatatg aggagataac aagataagat ttttttctac aaaaagaata
    28321 gaaaaattgt aaaaaaaact aaaaaaatgt attgacttgt tatttaaaag gtgatattat
    28381 tattactgta ctcgaaagag aacaaaa
//
LOCUS       DAYATN010000039        27788 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.039, whole genome shotgun sequence.
ACCESSION   DAYATN010000039 DAYATN010000000
VERSION     DAYATN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.039"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(133..1320)
                     /locus_tag="ACGI8A_001643"
     CDS             complement(133..1320)
                     /locus_tag="ACGI8A_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HFH0179644.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(1522..2793)
                     /gene="brnQ"
                     /locus_tag="ACGI8A_001644"
     CDS             complement(1522..2793)
                     /gene="brnQ"
                     /locus_tag="ACGI8A_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFH0179645.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            complement(2934..3587)
                     /locus_tag="ACGI8A_001645"
     CDS             complement(2934..3587)
                     /locus_tag="ACGI8A_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFH0179646.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     regulatory      3916..4017
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            4090..5433
                     /locus_tag="ACGI8A_001646"
     CDS             4090..5433
                     /locus_tag="ACGI8A_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFH0179647.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            5612..6976
                     /locus_tag="ACGI8A_001647"
     CDS             5612..6976
                     /locus_tag="ACGI8A_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFH0179648.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(7154..9019)
                     /locus_tag="ACGI8A_001648"
     CDS             complement(7154..9019)
                     /locus_tag="ACGI8A_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HFH0179649.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(9255..10472)
                     /locus_tag="ACGI8A_001649"
     CDS             complement(9255..10472)
                     /locus_tag="ACGI8A_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0179650.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(10672..11535)
                     /gene="aroE"
                     /locus_tag="ACGI8A_001650"
     CDS             complement(10672..11535)
                     /gene="aroE"
                     /locus_tag="ACGI8A_001650"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFH0179651.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(11617..13539)
                     /locus_tag="ACGI8A_001651"
     CDS             complement(11617..13539)
                     /locus_tag="ACGI8A_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0179652.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(13603..14451)
                     /locus_tag="ACGI8A_001652"
     CDS             complement(13603..14451)
                     /locus_tag="ACGI8A_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFH0179653.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            14864..15745
                     /locus_tag="ACGI8A_001653"
     CDS             14864..15745
                     /locus_tag="ACGI8A_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0179654.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(15953..16792)
                     /locus_tag="ACGI8A_001654"
     CDS             complement(15953..16792)
                     /locus_tag="ACGI8A_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HFH0179655.1"
                     /translation="MSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEYKYAVSQGD
                     YVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDELPLFLSRL
                     ECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARIVIESRDDV
                     SNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIADILCNPLS
                     RARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIVEPVGPWLE
                     KMR"
     gene            complement(16971..18905)
                     /locus_tag="ACGI8A_001655"
     CDS             complement(16971..18905)
                     /locus_tag="ACGI8A_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HFH0179656.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(19017..20030)
                     /gene="galE"
                     /locus_tag="ACGI8A_001656"
     CDS             complement(19017..20030)
                     /gene="galE"
                     /locus_tag="ACGI8A_001656"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFH0179657.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(20054..20929)
                     /gene="galU"
                     /locus_tag="ACGI8A_001657"
     CDS             complement(20054..20929)
                     /gene="galU"
                     /locus_tag="ACGI8A_001657"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFH0179658.1"
                     /translation="MQMTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIE
                     EAIESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHY
                     IRQKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGV
                     QKIAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSIL
                     EKQTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENL
                     KDDLMDYMRKKVSEK"
     gene            complement(21121..21441)
                     /locus_tag="ACGI8A_001658"
     CDS             complement(21121..21441)
                     /locus_tag="ACGI8A_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179659.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(21431..23422)
                     /locus_tag="ACGI8A_001659"
     CDS             complement(21431..23422)
                     /locus_tag="ACGI8A_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179660.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(23545..26088)
                     /locus_tag="ACGI8A_001660"
     CDS             complement(23545..26088)
                     /locus_tag="ACGI8A_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFH0179661.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(26366..26700)
                     /gene="rnpB"
                     /locus_tag="ACGI8A_001661"
     ncRNA           complement(26366..26700)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGI8A_001661"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            26832..27509
                     /gene="srtB"
                     /locus_tag="ACGI8A_001662"
     CDS             26832..27509
                     /gene="srtB"
                     /locus_tag="ACGI8A_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HFH0179662.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(27669..27743)
                     /locus_tag="ACGI8A_001663"
     tRNA            complement(27669..27743)
                     /locus_tag="ACGI8A_001663"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27709..27711),aa:Thr,seq:tgt)
BASE COUNT         8960 a         4837 c         3118 g        10873 t
ORIGIN      
        1 attaaaaacg aagctatctc aacatatgga taagcttctt tttaattgtt aaaaagataa
       61 aaatatattt gatttaacag aaaaagagtg cctcataaac taaaaagttc attttgagac
      121 acgtccttaa tattaataaa ttaattggct taaaattact gattgataat tataaagtac
      181 ctctgttaaa ggagtaaatt ctgaaccgat tccaaatctt aattctgtaa acgcctctaa
      241 ataatctggg tcacatacat cttcttgtgt taaatcttgt ttcaccattt cagtagcaag
      301 ataaattgct actgatggta gaaaagcatc cattggcata ggatatccac caagtctatt
      361 tgacatgcca aaagccttag ccttttcact tatcatgtcg tttattttat cataattttg
      421 agcatctttt tcagatattt ttagacccat aacactagga tatgtttgta ttggtgatgg
      481 attgctttgc tctgcaacta tatactttaa ttctaaagcc ttagtcaata taacttcatc
      541 catatactca tttacaccaa atacatttat atcttttcca tattttttaa cttgtttttc
      601 aatatcttca tttaaaaact gcttaacttt gtttttgtct tcttcagtat ttatatttgg
      661 agtattaact tgtacaaatg gcaatcctat atttttgcaa gtctctttaa tcatttctaa
      721 tcttttggct atattaacat ctttcaaatc atctgtagag gcataatgaa taaaagcttt
      781 tgctcccatt tcttttgaac gttcagctaa tacttttcct ctttcctctg cactaacccc
      841 tagattcaca tcaacaaatt ggcttaattg gtttttatca tctcccatag gggcagaaat
      901 agttattatt tctggacgct tttcctttac cttttgtaaa gcaggtaaaa gccctgcttg
      961 gtcagttgaa actacaatag cctgaacttc tttatcgtca gccaacttta ctatcttatt
     1021 aatagcactg tctatattag aagtaaaatt ctctggcaat acaacatgct ttatagtttg
     1081 tttaacttta gtttgaccat ctttgtcttt atcaatttta ttctcttcct catattcttt
     1141 tgccatctct tccaccatat tgtattgtac tttattttca gataatggtt gagttaccac
     1201 tgcaacttta aaatcatcta taattttctc tttatctttc ttgcctacaa gtaaactaca
     1261 acctgtaagt ccaataatta aacatatgga tagcataaat gctgttattt ttctaagcat
     1321 taattaaaac ctcctaaatt tgtacattct tatcactatt actaatttta tcatacttca
     1381 aagaaaaatt atattataaa aaattatata catgctatta taatccaatc taaaaagtac
     1441 tatgggtaga ttaggtttag attaaaccta cccatagtac taaaaattaa tatattataa
     1501 aattaaatta attacaaatt attatgctac ttgtttgttg ctttttataa agttaccaac
     1561 tattccaccc aatatagctg gtattaccca gttaaagccc aaacctgcaa gtggcaagct
     1621 ttgtataaat ggaacagctc ctgtcattcc atttatagct gataataaac taatcactaa
     1681 tgtaacatat gctgcacctt taaaagcatt atcattcttt atatgtttac cagccaactc
     1741 cataacaact attgctaata aaacaggata tatagtttgt aggattggaa ctgatatggc
     1801 tattatagaa tcaacaccta aatttgatat aagcatacta gctacacata tacctataac
     1861 cagttgttta taactcgcct tacttattcc ttcaaaataa gttgatattg atgctgttaa
     1921 acctgtagaa gtagtcagac aggcaagtgc cacaattaca cctaaaatta cctttcctac
     1981 ttgaccatat aacaatatac tagttatttc aactaataaa gatgcttgag gtatactagt
     2041 atcatataaa gttgaaactg ttgcacctaa ataagtaaga ccaccatata ctactgctag
     2101 aaatacacag gctacaaaac cagctttaat agtcattcct atttggtctt tatctgatgt
     2161 atatccttta gctattatag acatcaacat tatagttgcc atagatgctg cacctagagc
     2221 gtccattgtt tgataacctt gtgatatacc ttcaccaaat agatttttat ctatcatatg
     2281 ctcaggactc ataggtcctg ctggagaaat tatacccttt actattaaaa caaataatac
     2341 taataataac gctggagtta aatacttacc tattatgtct ataaccttag aagacctaat
     2401 agtaaatgct aaagttagcc caaagaagat tattgaaaat acagctttag ctactggacc
     2461 atggtctcca aatataggca taatacccat ttcatatgtt gttgcgcaag ttcttggtat
     2521 tacaagaagt gctaaacatg ctagagcagc tacactcatt actcttgcta accctttacc
     2581 agctctccta agtacatcat ccatgcttcc cttacatctt gctgttgcta taataacaag
     2641 cattgctata ccagcatctg caacaacaaa tccaataaga ggaattaacc agtctttgcc
     2701 ggattccatc cctaaaaatg gaggaaacat aagatttcct gcaccaaaga acatagaaaa
     2761 taaagcaaaa ccaactataa aaatatctct catctgctta ctcacactaa attaccccct
     2821 ttgaaaatat tttgaatatt aagataatct tactttattt tcagaaaaat gtcaatctgg
     2881 taaaaattca cacattgtta acgttttccc aatttatttt atgaatattt ttttcatttt
     2941 atgataattc ccatcaattt cgttaaatca attgcttgtg acacatcaaa aattgctcct
     3001 ctaatatcat ctataccaat ctcaattccc tcaatattat tagtagtaaa atctatgcca
     3061 gaaagtttag ttccattaaa gtttgctttt tttaaatttg tatactcaaa tactagattg
     3121 tcacctttta cttcttgaaa tacagaattt tcaaaatttg aatctaagat agaaactatc
     3181 ttaaatttag agaatgaaaa gttagaatac ttacctaaaa catccttgaa caaaacgttt
     3241 ttaagtatac aatcatcaaa tctaactcct atcaatttac tatttttaaa ttcaactctg
     3301 tatatacttc ctccatcaaa aaatgtattt gataaattgc agttttcaaa taccacatca
     3361 agtaaatcaa tcttttttaa attacaattt tcaaaagaca catttctaaa tatacaagat
     3421 tcaaagctaa ctcttacaga atctaaatca cttataactt cattttctat aattttatta
     3481 aatatttttt catcattaaa tatatcttca agtatatctt caagattttc aataatttct
     3541 aattctcctg taaatttagg tttttgcact ttaataattt tagtcatttt cccctccact
     3601 ataaattata ttatttttga tattattttt tttaatatat taacttaata tacatatttg
     3661 atatattaaa aagtttttac gaaatatcca tgtattcagt acttttataa ttatatcaca
     3721 aactcattta taataaactt ccattaatag tataaaacaa gttagaataa ttgacattat
     3781 actcatacat attcaattat tggaaaaagt aatttttttc aataaataac cttaattttt
     3841 tataaaatcg acaaaaaaac gaataatttt attatattta ttgtaaataa tacaatatta
     3901 cgatataata caacagaaaa acaaatagaa aatgttcata taatattagt aatacggtct
     3961 aatagtttct acaaactcac cttaaatggg ttttctatga atagaatgtc aactctgaat
     4021 atatgttcta ttctatctat atgaatatat atttttttgt ttatatctta aagttttttg
     4081 gaggtaaaaa tggtaccaaa cactaacaat gtacaaccag gtggaaataa ttcatcatta
     4141 agctttttag acaaaatgtt taaatttagc gaacgtggct ctagccttaa aacagaagta
     4201 ataggtggag ttactacatt cttaacaatg gcatacatag ttttcgtaaa tccagcaata
     4261 ttatctgaag ctggaatgga taaagccgcc cttataacag ttaccatact agctacagcc
     4321 attggtacac ttatatttgc gattttagga aatgcaccat ttgctctagc tcctggtatg
     4381 ggattaaatg catttttcac atactctttg gttataggtg caggaatccc atggcaaaca
     4441 ggtcttgggg tagtttttct atctggatgt gtcttcttta cactcgcagt aacaggtctt
     4501 cgaaagacaa ttgcagacgc aattcctaga gaattggcag tagcatcttc agcaggtata
     4561 ggtcttttcc ttgcttttgt tggtttaaaa aatatgggtg ttataatagc taatccagat
     4621 actatagttt cacttgcaga ttttacacca acagttttaa tagccctttt tgctcttatc
     4681 ttaatggcaa tactagaata taaaaaagtt aaaggtggaa tattgataag tatagtagtt
     4741 gcaacaataa tatctgttat atttggattt gtggagttgc caacagcaat aatctcaaca
     4801 cctcctagta tagcaccagt tgcttttaaa ttagatattg taggtgcatt gaaattctca
     4861 ttaattggac ctatattctc tttcatgttt atagacatgt ttgattcatt agcatttctt
     4921 attacttgtt gtaaacaaat gggtcttgaa gatgagaatg gtgaaataaa agatttagga
     4981 agaatgttat atgcagatgt ttcttctact ttaataggct cattcttagg aactagtact
     5041 gttacaacat ttggagaatc tgcttctggt attgcagcag gtgcaagaac aggacttgct
     5101 tcaatagtaa cagcagcatt attcttagct acacttatat ttacaccatt acttggtata
     5161 gttcctactt acgctgcagc tcctgcactt gtaatggttg gtgtgtttat gtttgaaagc
     5221 ataaaacaga ttgattttgc tgatagcaaa atagctgtac ctgcttttgt tacagtttta
     5281 ttgatgcctt taacttatag tataagtatt ggtttatgct ttggatttat ttcatatata
     5341 ataatgcatg tagtagctaa agaaactgat aaaatcagta ttacactttg gattataggt
     5401 ttacttgcag ttgtaaactt agttctaagt taatattttt aaatagttct aggtcaatat
     5461 tttaaattat atagaataaa atcttattta ttttaaattt ataaatagca aaatcacttt
     5521 atatgtaaat tgattttgct attaactttg tatagaaaat ttattatcat aatatataga
     5581 tataaaaata tatagaaaag gagtggtgtt tatgtcacaa ttacttataa aaaatgcata
     5641 tctaattact atgaatgcag atagagaggt atatgaaaat ggaagcatac ttattgaaaa
     5701 taatattata aaagaggttg gtaagtttga tattaatatt gtagataaag atgcagagat
     5761 atatgatgct aaaggaaaaa tactaatgcc agggctagtc aatactcatg ttcacttatc
     5821 tcaacaatta ggacgtggtc ttgcagatga tgttgtactt ttaacttggc ttagagaaag
     5881 aatttggcca tacgaaagta gcttcaatta tgaagattcc ttgatttcat ctactgcatg
     5941 ttgtgttgaa ttgataaaat ctggtgtcac tacattttta gaatctggtg gtcaatatgt
     6001 agatgctatg gttgaagctg ttgataaaac tggacttaga gcctgtttag ctaaatcagt
     6061 tatggataca ggagatggtc ttcctgaggc atggcaaaaa actactgatg aggaaatcaa
     6121 cactcaatta gacttattcc ataagtttaa taatacatct aatgagagaa ttaaaatatg
     6181 gtttgcatta agaactattt ttaatacaac tgaagaatta cttgttagaa ctaaagaact
     6241 agctgataaa tacaatacag gtatacatat gcatattgct gaaatagctg atgaaatttc
     6301 atttgtaaag caaaacaatg gagttggaac agttgaatac cttgataaac taggtgtatt
     6361 aggaccaaat cttttagctg ctcatacagt ttggcttact cataaggaaa ttgatttatt
     6421 tagattacat gatgtaaaag tttctcatga cccaggttca gctatgaaag tggttctagg
     6481 gtttgcatca attcctgaaa tgttagaaaa aggaatacct gtttcaattg gtactgatgg
     6541 agctccttca aataatagaa tggaccttat gagggacatg tatcttacat cactaataca
     6601 taaaggaaga actttaaacc caactgtcgt accagctgaa gaagtcttag aaatggctac
     6661 tataaatgga gcaaaatgtg ctttactaga aaaagaaatt ggtagtcttg aagtaggaaa
     6721 aaaagctgat atgattattc taaatccaga tacaattcat tgtttaccaa tgcataatcc
     6781 aattggaaat atagtttatt ctatgactag tgaaaatgta gactctacta tttgtgatgg
     6841 taaatggttg atgaaggaaa gaaaagtatt agttgtagat gaatctgaat tattaaataa
     6901 agttaaaaaa caagcttcta aaataaaaga taaagcagaa gttaaaattc catctaaatt
     6961 taaaattata aaataacata aatttcaatt gttttgtaaa tatgaatata taaatatatt
     7021 tatatacatc atatatacaa aacaattgtt tttttatttt atattcatta gcattgctca
     7081 taaaatacac cataattcca gaaaaaataa ccaatcagtt aggattacat catctaagca
     7141 atatataaac gtcttagttt ataagtttta ttctactctt atctaaaacc atctttaaag
     7201 agttttcaac tgtttcaatt acatctttat aactcaagta cacatttgcg tagatacaat
     7261 cttcatataa attatattca gttactctac aatacagcag ttttcctcca tctaataaat
     7321 tgctcaaaat atagttttct tttcttggga catagcctat cacatatcca gtttcaccaa
     7381 ctattttaat agctttttca tcatatagat tatctttctc tctagttaaa tagtatatct
     7441 tacttttctc caattctttt tcaagatatt ctctatttct aaaccttgaa ccagcaacag
     7501 ctattgaagt caaatagata tccttaccat gttcatctac ttttattttt tcaatattta
     7561 caaattcttt ttttaaatta ttactttcat ataatactct tcttaattca tccttcaata
     7621 taacattttt ttctttttca tacgcttctt taacaatttt taattcttca agagatagtt
     7681 tctctctgta tctttttaag atatttatgg attcttttct tacttcattt atatttccaa
     7741 atattccttt taaagatata ttctttcctt caggatatag tgatttactt cctttaagca
     7801 caataaaaaa taacatatca tcataatatt cttgagtcaa tttatctttt tctatatctt
     7861 tcatttttcc aattaaatca tctattttaa acgtctcttc aaaaaaattt aataatttta
     7921 atctagaact tgtcgttccc tttttagata tatgccaata tacctcaaaa tctctaatat
     7981 ctctatttag atatggcaac aagtcatcaa aagataagtt tacatcataa atttctgaca
     8041 tttttataat atcttctgtt tttccactag catctcttaa tgcttcaaaa tatatatttt
     8101 tccatttttc actgagcaag ataccattta tttctttttc aaattcaatc ttattgcact
     8161 ctatattttc atcttttaag acgtttatag ctattaggaa aattgaatat aaactttcaa
     8221 agttagtccc ataatttact acagtaggta aaaatcgatt cactaattct aatttattac
     8281 ctatatattt aaattctaca cttagcaagt aattgcatat aagacgtgct aaattattta
     8341 tcttttcttt atctaaatct cgtttttcaa gatattcatt caaatctaca attgaaataa
     8401 tatagttcat caataatcgt tcgtactttg tatccttata accatcttca attagatagg
     8461 aatttatttt agaatctaaa ttttccatat taacaatagc aaatactttt attgaacctg
     8521 tagccttttt agataaatta aataaatatg tattgtaaaa ttcaagtttt ttaatagtat
     8581 tactcagata aaatacgtat tctccagatt tagaaaatgt atctacaact tctcttaagt
     8641 tttctacatt tagatatttc tcacttaaaa ctaaccctag ttttacatac tcaacttttt
     8701 tactcttata tattaattct ttagttaatt tatataacca tttctcatca ataatcattt
     8761 cttttaccaa agtatttatc tttttcataa aaatttcgaa ataacaaatt aaagacttag
     8821 aattactacc ctccaataaa taaatttcat tagattcact tttatacata tttaaaattt
     8881 catctactag attatttgca agctctactt gttctcttaa cggtatactt tcatatatac
     8941 ataggtaatt catttcaaat gattctttgt atccatcata accattaaat tcctgcaatc
     9001 gcttaaaaac attctccacc aaattatcct cctttacatg atgtaattta tattgattat
     9061 actacttatt tcatcaagtt tacatttata cgcaaaaaaa tattactaga aattgaaatt
     9121 ctagtaatat ccttattatt aattatgtaa ataccctatc ttataactgt aaataatatc
     9181 cttattaatt ttaattactt ttaataaaaa tctatcctag atttagactt ctctatgcat
     9241 atattatact aatattagac atcataatca tacacttttt ctttttcata tctcatgttt
     9301 acaaacaatg caagtaaaat tccaacaaat gttattgcca cattaaacaa tacaacatat
     9361 tttggtccat taactccccc tgattttgta ataatacctg ctatatttaa aataacatag
     9421 tttgctatac tagatgcaat cataactata gatgttattt ttcctttatt agttggaaac
     9481 atttcatttg cagttgacgt tgttaactga agaacacctc ctgctgctga ataacctatt
     9541 acaaatcccc ctaacataca aatagttgga gtttgaacaa aatatacaac taatagcata
     9601 agtaaagcta ttataggata tataactaat attcttattg gtttaatata ttttttaata
     9661 agtaaagaag ttattaatac tgcacatatt actcccattg agtaaaagga ttgaattttt
     9721 gtagggtcag ccatcccata caatttaccc aattcctgat tacaatttaa ccataataca
     9781 aatgtagatg tacatgtaaa tccaatacaa acaagagcta tacttgttgg tgtaaatttc
     9841 atcttctctg atttaactgt tttgcctttg ttatcaataa cattattagc tggtggaaat
     9901 ggtaacacag ctatcaaaaa ggcatctact acaataagaa tcgctgttac tataaaaata
     9961 actttaaatg atattgaatt tgctgcaaca aatccaatta taaaaggaag caaaaattgt
    10021 gctaaagcta ttgtaaattt agtaaacata tttgctattg ttcctaaact agcaaatatc
    10081 tccatacaag aaggagttac acaggtatct aagaatgaat ttgctgcccc tccaattatt
    10141 gcaaatacat atgctatata aaaatttggt gaaaatacaa ttccaacaaa gaaaattgca
    10201 tgtagaaagt ttcctataag accacttact tttcttccat acttatctga aataggtcct
    10261 gcaaatggat aagatataag tctccctaat cctagtgcag caataacagc taaaacaata
    10321 cttacatcca atgttccatc tgataatgtt ttagcccccc acattgatga aaattctggt
    10381 ttgtattgtc ctaaaattga aattccaata ccaagtaaaa aatatgaaaa atacaacgct
    10441 atggcagtag gataatattt tttattgttc ataatttttc tcccattctt gttaatttat
    10501 taacattttt gtgtacaata aactcatccc cataatggat ttaaagtata gtcagttaaa
    10561 gcttcaaatt taactggact atgaggcaaa acccactatg acaatttgta atttattatt
    10621 aaaaatattg aaatacgtat gttttttcat tgtttaagat attactctta tctatttaaa
    10681 gcataattca ttaacttctt ctacaggcat ttctaatcca gtatatagat taaaagcttc
    10741 tgctccttgg tataataaca ttcctaatcc accaatagcc ttacatccat tagcttcagc
    10801 atcttcaatc atttttgttt tcttaggatt ataaacgaca tctgttacta ctaaatcttt
    10861 tcttaaaaca ctagtatctt ttatatttgt ttcattgtca taaggcttca taccaataag
    10921 agttgcattt gtcaatatat cacttgattg tatctcctca taaagtttgt ttgtatcttc
    10981 taaatcatat agattaacaa cacattctgg tacatctttt tttatattct caactgtttg
    11041 ctctgctctt ttataaaaat catccttagg attaaagata gatatttctc ttgctccatc
    11101 taaagcacat tgtacttgta tagcagttgc tgcaccacca gcacccataa ttgtaatctt
    11161 tttaccttta acttctaccc cattttcttt taaattacga acatagccta ctccatctgt
    11221 tatatgacca actaactttc cattgtcatt tacaattgta ttacatgctc caattatacg
    11281 agctgctgga gaaagttcat ccatatattc tgtaacagca gatttacatg gcatagtaac
    11341 attggctcct tttatattaa aagtttttat tgctaaaagt gcctctttta ccttatctac
    11401 tgtgatatca aaggctaaat atctataatc taaatctaat ttcttaaaac tataattata
    11461 catcactggt gatttagagt gaccaaccgg tgtacctatt aatgcaaata atcctgttct
    11521 acctgaaatt tccataacta taatctcctc tcaatattat cattttcttt gcaaataacc
    11581 tattcagtta tttacaaaga aaaatttatt tattatttat atatttaatg ctacttcaaa
    11641 agcttctttt gttgcatcta atgccctacg agcttttact gcatcaccaa caatataagt
    11701 ttcttttaca acttctttta taacttcact taatgggtcg taattacgag aacccatagc
    11761 aagtactact gaatcaaaac cttttaaatc atgctctgtg ccatctgtaa gtaaatagtc
    11821 gacaccatca tcataaaatt tagaaacttt tgcaccagta atggtatgta tattataatt
    11881 ttcaaaatca ttcataagga atttacggtg ttctgaaatt acatctgcac caacttcttc
    11941 tctaagctca ataacagtaa cctcatgttc ttgttctcca aggaaggctg ccacttcaca
    12001 tccaaccatt ccacctccaa caacaagtac ttttttccca caagattttt taccatcaag
    12061 aaggtctaca gcatgaatta atcctgaatc attgattcct ggtataggaa gtacaagtgg
    12121 agttgctcca gtagctataa taactacatc aggtgatttc tcctttataa gttctactgt
    12181 agcttctgta ttagtacata tttttacacc atattgatta cattttgaaa tataacttct
    12241 aactaagtta gtaatatctc ctttccctgg aggatatgca gctaaacgca tttgcccacc
    12301 aagaacttca gatttttcaa ctaaagttac attatgtcct cttttagcac atacccatgc
    12361 tgcaagcata cctccaacac ctccaccaac aacaagaact tctttgctaa tttcagctgg
    12421 cttaagctct gcttcatgtc ctaatatagg atttgcaaga caggctatag gtttgccttg
    12481 gaacatattt ggtacacatc cttgtaagca tgcaatacat ggtatcattt cgtctagttt
    12541 tccattttta gctttattag gcatttctgg gtcagccaag ctttgtctac cgaatgctac
    12601 taaatcacat cttccttgtc tgaccattaa ctcagcaaac tgtggctctg tataacgtcc
    12661 aactgttatt actggtatac taacagcttt ttttatttca gtaactaatt ctgcactgaa
    12721 tccagcatgt aaagtcgtag gagcccacat atattcatca tgtatatgta tactacgtga
    12781 aacatgaagt ccatctattc cacaatcttc tagatatgct gctattactg aactatcgtg
    12841 tacatcaatt cctcctggaa catcatcagt gctgtttata cgacataata ttgctagaga
    12901 atttcctact tttttacgta tattttcaat gataagacga ggtaatctca ttctattttc
    12961 aaaacatcca ccaaactcat ctacacgctt atttgttctt tgtgatataa aactactaac
    13021 cagatatcca tgagcgcagt gaacttcaac agcatctgca ccagcttttt gtgcacgtaa
    13081 tgcagcatct ccataagatt ctataagttc atatatttct tctgttgtta ttgctagtgg
    13141 tgtattacga ccatttgatg caggtattgc agaagcagct tttaatggat gtcctgctac
    13201 tttttcattt ccttcagaac cagcatgttg taattgtata gataccttcg aacctgcatc
    13261 atgacaagca tctattacat ttttaaaact atctattgtg ctatcatcat ataaacaagg
    13321 cttacgagga cctccttttg cttttttgtc tacaacacta gattctatag ttattaatcc
    13381 aaaaccacct aaagcacgct caagataata agccttagaa gtttcactta aagtaccatc
    13441 tgtgtttgca aaattgtttc ccataggggg aactacaaaa cgatttggaa ctgtcattga
    13501 accaattctt ataggtgtaa acatagaact aatcttcata aggttttcct tcctttcaag
    13561 ttttatatta taatactctg tatattttta tattatataa gtttattttg gagatacttc
    13621 agaccatgct ttatccaaaa cttttttagt tgcattaaat acagtttcag cagcagtgga
    13681 taacccttta gatattagag aatcagatat tacttctgcc cctatgactc ttggtgatat
    13741 attgtgttct tttattattt tcacaaattc ctctgtgttt ccatatcctt cacctggagc
    13801 caaacggtca tgtagtgatt catccctaag tttttcataa ggagtttcta aaacatcaca
    13861 cacttgaatt gatactatct tatctgctgg aacaggtgca aggtcctctg ctgtctgctt
    13921 tgctctagcc cagtgccaag tatctaatat aagcattgca ttttcacgat tacatccttt
    13981 cacaacacgc catgcagtag ccaaatctgg tacaccacta tatggcataa actcaagacc
    14041 tataataagt tcacctgctc tgtcacataa ttctcccaat gcagttataa tttgttcttc
    14101 tggtatcatc tctaataatc cacaatttat atgtttaaca ttaaataagt gagccatatg
    14161 ataaacattt tgctcttttt ctctttgagc ttccgttcta tcttcttctc ttccccattg
    14221 agttatatat tctacttctg taactttcat atcatattca ttaagaattg caatcatttc
    14281 ttcatctgta acacctgcat tttttgcatc aatataattt tcagcacgca atccaatacc
    14341 atcaaaccca gcttcttttg caatacgaac tctatcacgg aatgaaactt ctgttcccaa
    14401 tgtataagaa ctaattgtaa ttggaatatt aagatttttt tgattattca tagcttaaat
    14461 ttgccccctt tattttcttt ataattaata gtatttttga ttttatattt ttatttatcc
    14521 tactagtagt attattttac cttaaaaata aaagtatttt ttgatttcta tttatctaga
    14581 ttttataaat ttattgtatc acgagacttt actaataaca aattgataat ttcctattaa
    14641 ttcatagatt ttgtttatag attttattta taagataatt cttgtatttt tctattaggt
    14701 cgtataaata tatttatctt ataattcaga caaaaaacga taaatttttt taaaagcttt
    14761 tattcttact ctataatcat atataataaa aatgtaaaaa tatcttaaag gttaacttct
    14821 acaaatatat aaataaaaat acctatgaaa ggatgaacaa aaattgaatt tatatcactt
    14881 acgctatttt gttacattgg cacatttaga acactataca aaagcggcag aaaatttatc
    14941 tataacacaa ccaagcttaa gtcatgctat ttctttacta gaaaatgaac ttggggtggc
    15001 tctatttgaa aaagaaggta gaaatatagt ccttactaag tatggaaaaa tatttttaaa
    15061 agatgtggaa aaatcattag aaattttaga ttcaagtgta aaatcattaa aaataactgg
    15121 tactggtgaa ggacaaatag acttagcttt tcttagaaca cttggtactg attttatccc
    15181 agacatagtg cacaaatttt taaaatctaa tcctgctaaa tcaatagatt ttaaatttca
    15241 tactggtgta actactgaca ttattcaagg tcttaaagaa agaaaatatg atattgcatt
    15301 ttgttcgaaa cttgaaaaag aaaagggaat tgaatttata cctgttgcca aacaagattt
    15361 agtactaatt gtaccatata gtcatccact agctgcaaag gatactatag atttaaaaga
    15421 aaccatacct tatcctcaaa tagtatttaa tcagagaagt ggtcttagat atataattga
    15481 tgatatgttt aaaaaaataa atcaacagcc taatatagtc tatgaagtag aggaagacca
    15541 agtaattgct ggattagttg caaaaaattt cggaattgct gtagtcccaa atatgaatat
    15601 gcttagcttt acaaaagtaa aagtaattca gattattcac ccaagttggg aaagaaactt
    15661 ttacttagca tttataaaag atagatattt gcctcctgca attaaaaact ttaaaaactt
    15721 tgtcattaaa aacgctcaat tataatttat taataaaggg gttattttta aatatcctct
    15781 tttattcttt ttataaaata aaaaaatcta tacttttaaa agtatagatt cctaactgac
    15841 agtcggcatc cgagtctcag taattatcgt tatacaacag caactggatt cagttcctga
    15901 ataactaggt tacttacatt atatcagatt ttttaaaatt tgtcaattgt atctaacgca
    15961 ttttttcaag ccatggtcca acaggctcta caataggaac ttctttaaat ccccaatttc
    16021 ttatatcatt cacactcgtt ggagtatata ttttgtttct gacagatttg aatattttgt
    16081 ttttttctcc atattgcata cactttggat tagcttctat taaccctctt cttgctctgc
    16141 taagaggatt acataaaata tctgctattt ctaaaccttc tctatagtca gcatcttctt
    16201 ttttggcaat tataaaaccc ttaaccttat ctcttaattc ttcatctata tctaagtata
    16261 ttgttccatt attaaataca tcaaaaaacg ccttttgtac ttttaagttt gagacatcat
    16321 cccttgactc tataactatt cgtgcagatt ctacagtttc attatttaca aatgaataaa
    16381 aattctgaag aacatgtata aaagccacaa catacggttc ctttgaaggt gcataatatt
    16441 tctttaatct agctttatct actgttactg atattatagt acactctaat ctagatagaa
    16501 ataaaggcaa ctcatcatag aataacttaa tctgtgaatc atcaaacttc tcaaatgctc
    16561 ttgattttct tgatatatct gtcaaatgta ttcctatgtt actggttcca aaacaatact
    16621 ctttaaatgc atccatttct ttcctcaaag aacatacata atcgccttga cttactgcat
    16681 atttatattc aaaaattact cctgtcaatg taaatgggtc ttcatgattt atatcagctc
    16741 ttccactttc atcaataaac attatatatt ttttctttat aatcttactc accctcttaa
    16801 catatgatga tagcatctat attcatttta tatgagtttt aaaaatattc caagattttt
    16861 atataactta aacttaattt tatactaaaa aattgagatg taaaacaatc aagcttttat
    16921 tgattgaatt tcacatctca attataatta tttttcaata tatattttta ctattttgca
    16981 agaatatcca ttaatttatc cattactata taagatactc cgcctcctat ttgataagga
    17041 gtttcaactt ttttatttgc aaatactctt tgttgctctg atgataatcc tgaattatcc
    17101 attaaaacta ttggtgaatg taattttaca gctaatggtc cagctgataa agcatctact
    17161 aaagcatctg atttactaac taatacagcc tgtaaatcag tatctccata gaatttttct
    17221 atcactttgg cattagtatc tactctacta tctccatata ttctatttcc tgaaatatct
    17281 tcagtagtta tatcattcat ttttgctatt actgaatcgt taattgcact tggtccacct
    17341 ataaagtatg cattttctaa ttttctttct gttagccatt tataagattc ttctgtaatt
    17401 ccatctttat tgactaatat tataggttgt ttttcttccc cagctttaga agctacagat
    17461 aaagcatctg cctccccatt tccagaaacc atatatgcag tttttatttc ttctcgattt
    17521 ccaagttctt cagctattct tgtagcagtt tgatatctat cttgacctgc tattctcttt
    17581 gtttctgtgt tgaaagtagt ttttatttct tgctctaatt tagaacttat tgcattttct
    17641 ccacctatca agattactgt tttaggtgct aataacttca tttcttcaac agtttcagtt
    17701 ggtaaattag ctgtattagt aagtagtatt gttgagtcat agctacttgc taaaggagtt
    17761 gctgttatac catcagctat tgcattacca tttactaata ttacagtatc tgaactatca
    17821 gcattccagt ttccaactga acttatcttt ttagctgtat caaatctatt tgcacctata
    17881 attgtatccc atgtaggatt tatagttcca tcttcattaa aagtataatc tttcccatct
    17941 attttttgag aaccagttag catctttcca tctttgtcta aatagtaaga cttaccatct
    18001 atttgttgcc atcctgtctg cattattcct gttttgtcaa agtaatactt attaccatct
    18061 atttcttgcc atcctgttac tgctaaacca tcagatccta gatagtattt ctttccattc
    18121 ttttcttgcc atcctgtttg cattactcca tctgagttaa agtaataagt ttttccgtct
    18181 attactttcc atccagatac tcttgctcca tcagttccta aataatattt tttgttatta
    18241 attgttttcc atcctgtttg cattactcct gaagcatcaa agtaatacat ttttccattt
    18301 acttttagcc aactattctt ttggtatgtt ccatcagatt ttacataata cttcttacca
    18361 ttttctgttt tccatcctgt ttgatttaga tttatcttat attttgccgc tatctttaca
    18421 tagctgtcgt tactataatc tataattaca tctgaaccta caggaacttg gtcaaataac
    18481 catctaacat ctttattgtg cattcttata catccaccag aaatatgctt tcctatagaa
    18541 ctctcattat tatttccatg tattccatat gtatctccat aagttccttt taatgctaat
    18601 cccatccatc tatctcctaa tggatttctt ggacttcctc caggtatatt acctttataa
    18661 tatggtctat tttttatctt gttaactact tttgttttcc ctgttggtgt ttctgacccc
    18721 tttttacctg ttgcaactct aaattctttt acaagtttat tatttacaaa atatcctaga
    18781 gtattttttc ttgaatttac tataatcaca tgtttactgg ctgcgcttga ttctggtacg
    18841 ccacatgcaa atactaaaac taaagtcagt gctagtacta aatacttttt tagacttttt
    18901 ttcactataa cttccccctc ttttagaaac attactacat gtgtctaatt ataacataaa
    18961 attaccaata ccttatattt acaaatttgt tacatttata catataagta tgtattctac
    19021 tcttcatacc cattaggatt catactttgc catctccatg aatcttcaca catctcttca
    19081 agttcatact tagctttcca tccaagctct ttttctgcct ttgaagaatc tgcataacac
    19141 atggctacat ctccagccct tcttcctaca atcttatatg gtatttcttt tccacttgct
    19201 ttagaaaatg cttttactaa atcaagaaca ctatacccct ttccagttcc taaattataa
    19261 acaacaagtc ctggattttc ttctaatttt tgtaatgctt ttacatgacc agcagctaaa
    19321 tctaatacat gtatatagtc tctaactcca gttccatcat gtgttggata gtcattacca
    19381 taaacactca attccttcaa tttacctact gctatttttg ttatataagg cattagattg
    19441 ctcggaactc catttggttc ttcacctatt ctaccacttt tatgagcacc gactggatta
    19501 aagtatctta aaagagctat atccaatgac ttatctgctt ttgatatatc tactaacatc
    19561 tgctctatca taagtttagt tcttccatat ggattggtta cagaaagtgg aaaatcctct
    19621 aatataggac aagtatgtgg gtctccatat acagtagctg aagaactaaa tacaaacttt
    19681 ttaacaccat attctctcat taattcaaat aaatttaagg tacttattag attattgctg
    19741 taatattcaa ttggtttttc tactgattca ccaactgctt ttaaagctgc aaagtgtata
    19801 attgattcaa tattattttc tttaaataca acatgcattt catctttatt tctgatatca
    19861 atattataaa attttactgg ttttttagat aattccttga ttctatcaac cactattgag
    19921 ttgctattac ttaagttatc aactataaca acttcataac ctgattctaa aagttctatt
    19981 gctgtatggc ttcctatata tcctgctcct ccagctacta aaactgccat tgtaattacc
    20041 tcttttctaa tatttatttt tcacttactt tttttctcat atagtccatt aaatcatctt
    20101 ttaaattttc tctctttaga gcaaagtcta ttgttgcctc taaaaaacca attttatctc
    20161 ccacatcata tcttctacct tcaaagttat aagcataaat agcctcttgt tttccaagag
    20221 tttgaagtgc atctgtcagt tgtatctctc cacctttacc aggtgtctgt ttctctaata
    20281 tgctaaatat tgcaggagtt attatatatc ttccaagtat agctatatca gatggagctt
    20341 cttcaattgc aggtttttca accatatctt taactttata tactctatct tcgatatgtt
    20401 ttacatccaa tataccatat ttattagtat cttctttagc tatcttttga acacctaata
    20461 cagttgtttt atactcatta taagtatcta ttaattgttt taagcacgga acatctgaat
    20521 ctactatatc atcacctaat aatacggcaa aaggttcatc acctataaaa ctttttgcac
    20581 agtaaattgc atgtccaaga ccttttggct ctttttgtct tatataatga atatttacca
    20641 tatttgatat gtctcttacc atttctaaca tctctgtctt acctttttgc tctagttcta
    20701 actctaattc aactgattta tcaaaatgat cttctataga ttttttattt ctacctgtta
    20761 ttataagaat ttcttcaatt cccgattcta tagcttcttc tattatatac tgtaaagttg
    20821 gtttatctac tataggaagc atttctttgg gctgtgattt tgttgctggt aaaaatctag
    20881 ttcctagccc tgctgctggt attattgctt ttttaactgt catctgcatt ttttcaccct
    20941 tcttcgtaaa ttttctattg taccaaatta atataccata tttatacttt ttcttcaaac
    21001 tctatattat cccaatattt aatgctataa gataatataa ggtagatgaa atttcatcta
    21061 cctttagttt tataagttaa tattgtattg tttatatttt atccaaaact tatacaggta
    21121 ctaaatttca tctaagatgc cttttatatc actatctata tcttcatcta gataagaatc
    21181 taaatcttct aagtcaactt ctagttcttg atatgtcata tcaacactga catcctcatc
    21241 tatatccatt tctgatatta attctcctaa aatactattt ccatcagaat ctaaaccact
    21301 atctatatct tcatctatat agttttcatt taaaatttca tctaaatcaa tctcgtaata
    21361 aacttcatta tttttttcat cccctgagtc caatcctaag aactcatagt taaaagtcca
    21421 aacattgtat ttacttgaca tactttattc ctcctgtgaa aaacatattt tcatctagtt
    21481 tattggttcc ataatcaaca gagttatata ttttttcaat tatgtctttt cttctttctt
    21541 ttgtactatt atttaatgtc aattgtttaa atacatatgt atttgttatt ctctcgaata
    21601 atatttcttt tatactatca tcatcaatat ttctatagtt tttccacaat aactcaaata
    21661 acttaaacat ttccctacta gtttcatcta cataataagg atacatattt tctactaaat
    21721 ggtttaatct tgatatggct tgttttaaaa atacatcaag acttgcactt tcactttctt
    21781 ttaacattct ataattgtat ttcttcttat cttctcctga aattacaaag atgatattgt
    21841 ctaaactttt ttcaacttca tttaaataac tgcttatatt atatgataaa tcagacaata
    21901 gatttttaat ctgcttaaac acttgtacat caagtttagt attatacttt tcaaacaaat
    21961 ctatttcttg attttctaat attcttggca tatatgctga caataaatct aaattgtttc
    22021 tttgttcttg tatttgctta taaagtgaat ttataacttc tattcttatt tcatcattta
    22081 actctttttc taaaatctta tcaaattcag atttcatata gtaaatattt ccaaagaaat
    22141 taaatattat catatcattc atagctttag accaattctt atatatcact tcaatagaat
    22201 tttctttaag tagaggttgc tttgcatata taaatacatc ttgcataagt ttttgaacca
    22261 tacttttact taagtttaaa agtacttttt gattattaaa ttttatttcc gtttctacaa
    22321 aattagaatt ttcgttttca agttctttat taattacaat gtcttttata tcactatttt
    22381 ttatagatat gatttctttt tgaaaagact ttagaatagt caagctatcg aaatcttctt
    22441 gaataaaaaa tggagcatgt tttaatgtat atcccactat ttcattttct atatctttta
    22501 ggtcatctat ttgcataatt tctctatttt tatcatttat atgtaggtta aatacatttt
    22561 tattatcata taatattata tcttctccta aatttaactt agaacaatta tctttcgctg
    22621 actttacaat agtcttctta gatttcttat ttataaaaat taattggtct tgatatttta
    22681 ctaaaagcat gtttgtgttt ttataatcaa atccatctat atttcctgat atcttgcaga
    22741 atactatttc acaatcttta ggaagatttc ctattttttg gttatcttta actctctcat
    22801 ttaaatcaga tgggactaca ctatctatat tgttattaaa tatagataca gaccttacta
    22861 taatcccctc tctttccata tgaaagtatc ccttcaatga cactctttta tcttctaaaa
    22921 tgtatctttc tatatcatca aaatttattt caaatctatt tttcccactg ataaatataa
    22981 acttctcatt atctttatta attccaccca tataaaactt attgtcatct aaacttaata
    23041 tagtcatatt atcctttaga tagaatataa attctctttg acttttgcag attaaatcat
    23101 attcatccaa agttattttc atagtcaaat ttgctgtaac atttttactt gaattttcac
    23161 tttttatgta aaaaggcaag ttatttccat agacaattat ttgagaatct tctaaaactg
    23221 cctctaattt ttctttattg tctgaaaaac tatatatata ttttacacct gtatcctcat
    23281 taacttcatc tgatttgctg attattatat tccctagatt tgttatatga agctttttat
    23341 attcttttga gtatctactt atgccactaa gctcaaaccc atctgaaagc tcatatatga
    23401 ttttttctcc taatttcaac ataccttcac cacctaaaca gaattataag tattttataa
    23461 aggtgaataa aatgtctcaa aacaaataat atttttttta atacttttta ttctgagaca
    23521 tagtttctcc ttttttataa tgctctaagc accttcaact atttcttcat aaagtttatc
    23581 taaatgttta acatattcac gatatccaga tttgttcttt aactcacttc ttaaatcttc
    23641 atatttttgt ttaagttctt cagcactcaa agaaggagct gattctttta attcttctaa
    23701 cttataatca tagtacttat catacatagc tatatatgta tcaatagact tcttagctag
    23761 ttcattacat ttttcttgtg cttctccata ttgagaataa tcacctattt ttattttata
    23821 gtatccattt tcttgaatta ttgttgaaga aataccttta gcttcatatt cttttgctgt
    23881 ttcttttgct gttttttcaa ctttaagtga acctgcataa acagtgtaat atacaccttt
    23941 accatcttta tatgcttcca tttcttttgc tttcttttca gcttgtgctt tttcttgagc
    24001 ctctttttca gcttttgcct tttcttctgc ttcttttttc tctttttcag cttgtgcttt
    24061 tttctcttct tcagttaatt tattgttagc ttgttgttga gttgatgact ttggaatatt
    24121 ctctttatca tttttcttat tgttgcctac tgttataaaa tatcctacac caacaataac
    24181 cactataact actgctataa tagtttcttt taatctactc ttcttagaaa caacctcttc
    24241 atcatctaaa gaatcttctt tatctgatac ataatttttc atttctttct tgttttctct
    24301 atatttgtcc ttagaagaat ttttagattt tttattacta ttttctttct ttttaatagg
    24361 tattactact gatttttcat tttctaattt ttcttctttt atttcttttg cttttttatc
    24421 ttctaagttt ttactttctt tttcaagctt cgattcatta tttgaagata ctttttcatc
    24481 taagttatta tttattggtt tttctatttt tacactacgt tcttcttcta tatgttgact
    24541 tggttcttct tgttttattt ctttcaattc attttgctct atttcttctg atttcacttg
    24601 atgattttca actggtttat ctaaaattat atctggagaa atatcattta aattctcttc
    24661 ttcttgttca gcttcttcat tgtctttttt aataaatcca aataatttag ccttcttttt
    24721 actcttttta ttttctggtt caattatatc ttctttattg ctgtcgtact ctttatctgt
    24781 agtatcttta tcttctacaa cgtcacctat atcttgatta ttactatcta cttcttcttt
    24841 agtcccttct tcagcatcat cttctattga aatactactt ctaaagcaaa tatctttatc
    24901 tgcatttttt atcatgtaaa tgtattcttc aatatcgata tctttatttc ttctagttct
    24961 ttttgatgca atttcatgat tttctaattc actatagtta tcttttatta gattctgtgc
    25021 tttattccat gtatcttcac taatttctgt tttattttta attacatagt ccacaaattc
    25081 cttaaaattt tcaggaccaa attctccaat tatagtcagt ctcattgcac cttctataag
    25141 gtctctattt cttctcttta aaaattctaa tacaactgtt gcacaagcaa aatctttaat
    25201 aatttctttg ccaaaaccat atgcttcaac agaatgaagt tcttctaatc cacataactt
    25261 cttatattca ttgtgtattg tttgagatat actttcatta tttaagctat ttaacttatc
    25321 tataaacttc gattctatat catttgattt tgattcatgt tttacttttg aatttttaat
    25381 ttcatttagt agcatgtcat tttctgaatt atctttacct aattgcaaat cctcaatttc
    25441 agttgctttt atttctactt tgttttgttc gttattctct tccacattat taggtaccac
    25501 atctttatta ctagctaata ttttgtttac acagaatata atttctcttt ttaattctct
    25561 tttcgcatct gcttgatttt taagaaataa atctccacat tctttctttt ctaaacttat
    25621 ttttctaaga gatatattaa gaaggtcttt atataggttt aaaaccttat aatccttttc
    25681 taaacgtctt cttctctctg atagttccat tttatcataa gctaaaaaac tatttaaatc
    25741 attggctttt agagagtcta atccctcttt catagcctta tttacatact ttctacattt
    25801 tgctgtagtt aactctaaat ctttttctaa ttcatctatt tcaggtatgt acttatctac
    25861 tatccatttg ccaataagtg gttctgtatt tcttgctaat gtcttataaa acaatatatt
    25921 aaattttaaa agctctgcat attttgcttc attaaattct actttttcaa atttcttttc
    25981 caaaacacca tctagctttt catttatttt agactcttgt tttaaaaaac ttgctgtgac
    26041 atttaaaaca gtgtttctta ttatattatt ttcactcaaa accttcactc cttaaatcaa
    26101 ttattttatt gtaatattta cattgtgcaa agttgctaat ttgattatga tatctaaata
    26161 attatttact aaatttgcta catcttatat catatcatat tatttacatc attttacttt
    26221 ttctttttat atctatctta tattatacca tttttaatat ctgtactaac ttatattaca
    26281 atattcctca aaattttaaa agtagattag acaaatttac aaataaaaaa acatcagttt
    26341 aaactgatgt ttttgactat cgaaataatt aagcaagtct ataagcggag ttctgtatta
    26401 gataatcatc tttctaggcc tattgtcacc aataggctca agcgacctac ctaccggaga
    26461 gtacgagcag cactctttat tcctatcttg gtcttgctcc aggtggggtt tacatagcca
    26521 actagtcgcc tagctgctgg tgagctctta cctcgccttt ccatccttac cacaatagtg
    26581 gcggtttatt tctgttgcac tatccttagg atcgctccca ctaggcgtta cctagcaccc
    26641 tgctctatgg agctccgact ttcctcatgt aagattacat gcgattatct ggcttactta
    26701 atatttattt tacttagtta tgttaacaca aattctttta catgtcaaag taatattata
    26761 ctaaaaattt aaatttattc aaaaatagtg taatatagat atataaacta catttaactc
    26821 gaggtgatct attgaaaaaa ttatatagaa tagtaattaa cattatacta gtattagtaa
    26881 tactttacag tggatttaat atatactcaa aacttaccaa atacaatcat gatactaaaa
    26941 tatcctcaga acttcaaaaa aaagaatata aaaaagagga tttatcaaag attaacagtg
    27001 attttaaatt ttggctttct gttgagaata caaatataaa ttatccagtt gtacaatcta
    27061 aagataattc ttactatctt gacaaagatt tttataaaaa agattctatt tctggtacac
    27121 tgtttatgga ttatagaaat aaatctattg atgacaaaaa tatcataata tatggacata
    27181 atatgaaaaa taaaactatg ttcaacaacc taaataagtt taaagatgct gacttcttta
    27241 aaaaaaataa taaaataaaa ataaccttga atggaaaaga atttttatat gatgtttttt
    27301 ctgcttatat tgtagaatct gattatgatt atctaaaaac aaattttaac aatgaatctg
    27361 attatcaaaa ttacataaat gatattactt ctaaatcatt gtataaatct cctattaagg
    27421 taaatagcaa tgataaaata gttacacttt ctacatgtac ctatgaattt gatgatgcca
    27481 gaatggtgat tcatggtaga ttgatttaaa actcacagac acagtttaaa acaataattt
    27541 taaactgtgt catcttttat acctataatt tgtaaattag accactaaaa gtatttaata
    27601 caaaataaca aatacatcaa acaataaaaa acaataaaaa aagactatgc taaaaacata
    27661 gtctttactg gagctggtga taggaatcga acctacaacc tgctgattac aagtcagctg
    27721 ctctaccgtt gagccacacc agcacaccta ttttattgta aattagcaaa actaattttt
    27781 aaaagtta
//
LOCUS       DAYATN010000040        27610 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.040, whole genome shotgun sequence.
ACCESSION   DAYATN010000040 DAYATN010000000
VERSION     DAYATN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.040"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            262..2280
                     /locus_tag="ACGI8A_001664"
     CDS             262..2280
                     /locus_tag="ACGI8A_001664"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0179663.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIR
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            2785..4797
                     /locus_tag="ACGI8A_001665"
     CDS             2785..4797
                     /locus_tag="ACGI8A_001665"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0179664.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5039..6952
                     /locus_tag="ACGI8A_001666"
     CDS             5039..6952
                     /locus_tag="ACGI8A_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179665.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            6945..9164
                     /locus_tag="ACGI8A_001667"
     CDS             6945..9164
                     /locus_tag="ACGI8A_001667"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFH0179666.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDNLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            9273..10103
                     /locus_tag="ACGI8A_001668"
     CDS             9273..10103
                     /locus_tag="ACGI8A_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFH0179667.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            10093..13560
                     /gene="addB"
                     /locus_tag="ACGI8A_001669"
     CDS             10093..13560
                     /gene="addB"
                     /locus_tag="ACGI8A_001669"
                     /EC_number="3.1.-.-"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HFH0179668.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13560..17387
                     /gene="addA"
                     /locus_tag="ACGI8A_001670"
     CDS             13560..17387
                     /gene="addA"
                     /locus_tag="ACGI8A_001670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFH0179669.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGCRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVNNPDSDY
                     YGEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17433..18644
                     /locus_tag="ACGI8A_001671"
     CDS             17433..18644
                     /locus_tag="ACGI8A_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFH0179670.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18631..22158
                     /locus_tag="ACGI8A_001672"
     CDS             18631..22158
                     /locus_tag="ACGI8A_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0179671.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKDNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            22289..23542
                     /locus_tag="ACGI8A_001673"
     CDS             22289..23542
                     /locus_tag="ACGI8A_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFH0179672.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            23691..24743
                     /gene="ytvI"
                     /locus_tag="ACGI8A_001674"
     CDS             23691..24743
                     /gene="ytvI"
                     /locus_tag="ACGI8A_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HFH0179673.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            24833..26059
                     /gene="pepT"
                     /locus_tag="ACGI8A_001675"
     CDS             24833..26059
                     /gene="pepT"
                     /locus_tag="ACGI8A_001675"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFH0179674.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            26266..27318
                     /locus_tag="ACGI8A_001676"
     CDS             26266..27318
                     /locus_tag="ACGI8A_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HFH0179675.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT        11740 a         2695 c         4871 g         8304 t
ORIGIN      
        1 ttgtattaaa tttaataaac ctacaattgt tatatgtttt tatttgtctt ttatagattt
       61 ttttggcaat aaatgatata gtgaagataa ttaaatatat tcaatatatt taattttttt
      121 attttttgtt tttataattt ttttagaagt ttatttttta tgtacttatt tataattttg
      181 ggtaaaaaca ctttgatata atttcaaaaa ttaaaaggtt aaaaaacaaa gtagaaattt
      241 atgttaaata aggagattaa tatgagaaga aatacaaaat tattaacaac agggattctt
      301 tcaatggcaa tcgtcgcacc tacaatggca tttgctactg aatctaatgc tatggaaaat
      361 aacgctgatt taaatataaa cttagagaaa aaaagtatcg ttttaggtag tacctcaaaa
      421 gtcagtgtta agtttaaaga aaaaccagat gcagatagca ttacattaaa gtataaatgc
      481 tatgatatgc cattggatac aactttaaac tataatcaat caactgagtc atatgaagga
      541 actatcaatt ataataaaga cccagaatat ctaaatgttt gggaactaca agggataaca
      601 ataaacagca aaaataatcc taaaacttta aacaaacaag aactagaaaa gatgggatta
      661 aatttaaaag actataatgt aacgcaggaa tgtataattg aagatataac ttctagaaaa
      721 gatgtaaata aatatttaag aaaaacttct gcacctatta cagaacttac aggaagtgat
      781 agatacgaaa cagcagttaa aataagtaaa gaaggctgga aaaatggttc agataaggta
      841 gttataataa atggggatgt aagtatagat ggcattatat caactccact agcaactaca
      901 tataatgcgc caatactttt ggttgaaaaa aacaatgtac ctaatagtgt aaaatcagaa
      961 ttaaagcgtt taaatcctag agatgtaatt ataattggag atgagaatgc tatttctaaa
     1021 actactgcta atcaaattaa atcgactgta aatgctagtc aaacacgtct aaagggctct
     1081 aatagatatg agacatcttt attgatagca aaggaaatag ataaaaatca tgatgtggaa
     1141 aaagtataca taacaaatgc taatggcgga gaagtggatg cacttactat agcagcaaaa
     1201 gcaggtcaag acaagcaacc aattatatta actgataaaa atagtattac agacaacaca
     1261 tataaatggt taaagagtga ggatttacaa aatgcttatt ttataggtgg ccctcaaatg
     1321 atatcaacaa atgttataaa taaggtaaat gatataacta aagataatgt tactaataat
     1381 agagtatatg gagcagatag acacgaaaca aatgcaaacg taataaaaaa attctataca
     1441 gatgatgagt tagaggctgt tttagtagct aaatcagatg tacttgttga tgctttagca
     1501 gcaggtccat tggctgcgaa cttaaaatct ccaatactta taacaccaaa gacatatgta
     1561 tctgcatacc ataaagaaaa cttagaagct aaatcggcaa ataaggtata taaaataggt
     1621 ggaggattga cttctaaggt aatgagctct atagcatcat cattatctaa acacaataca
     1681 actccaacag aaccaggaaa tagtgggggc aagacagtta tgattgaccc aggacatggt
     1741 ggttcggaca ctggaacaac aggtaaacca ttagggggta ttagagaaaa agactataca
     1801 ttaaatactt cacttgcgac aactgaatat ttacgttcaa agggattcaa tgtaataatg
     1861 acaagagaca cagataagac tttatctctt ggaaatagaa ctgctttatc taactctttg
     1921 agaccagatt tatttacgag tatacactat aatgcatctg atacaactgg gaatggtgta
     1981 gaagtatttt ataagcttaa agataaagat ggagggacta ctaaaactgt agctacaaat
     2041 atattaaata gaatcttaga gaaatttaat ctaaaaaata gaggtgcaaa aacaagaaca
     2101 ctatcaactg accctacaaa agattattta tatgttttaa gaaataatga tatgccagct
     2161 gtacttgtag aatgtgcatt tttggataat gagaaagata tgagtttact taatacatca
     2221 aataaagtaa aagaaatggg tacacaaata ggtaaaggca tagaagattc attaaaatag
     2281 tctacaatga gagcatagaa aaatttaaaa taaattaatc tgcaatttta aatttaaact
     2341 aaagctataa gataagaatg taattataat agtaataaag actgtatata cattgtgcat
     2401 ataagtgcga aatgaggtat atacagtctt tttatgatta attgtaaaag gatttatatt
     2461 atatgaagaa tttacatatc ttaaggctac agttaattgt caagagttgg agtattcatt
     2521 tgtaatacac aataaaagct taattactat attttattgt gtgtttgtgt gttttttgtg
     2581 gatttttatg tcaataaatg atatagtata gataattaaa tgtattaaat atatttaatt
     2641 tttttatttt ttgagtttta taagtttatt tttatatatt acttatatat tatttatatt
     2701 ttgggttaga tacactttga catattttca aaagttaaaa gattaaaaat aaagtagaaa
     2761 tttatgttaa gtaaggagat taatatgaga agaaatacaa aattattaac aacagggatt
     2821 ctttcaatgg caatcgtcgc acctacaatg gcatttgcta ctgaatctaa tgctatggaa
     2881 aataacgctg atttaaatat aaacttagag aaaaaaagta tcgttttagg tagcaaatca
     2941 aaagttagtg tcaaatttaa agaaaaacca gatgcagata gcattacatt aaagtataaa
     3001 tgctatgaca tgccattgaa tacaactcta aattacaatc aatcaactgg ggcatatgaa
     3061 ggaactatca attataacca agacccagaa tatctaaatg tttgggaact acaagggata
     3121 acaataaaca gcaaaaataa tcctaaaact ttaaacagac aagacctaga aaagctggga
     3181 ttaaatttaa aagactataa tgtaacacag gaatgtataa ttgaagatat aacttctaga
     3241 aaagatgtaa ataaatattt gagaaaaact tcttcaccta ttacagaact tacaggaagt
     3301 gatagatatg aaacagcagt taaaataagt aaagagggct ggaaaaatgg ttcagataag
     3361 gtagttataa taaatgggga tgtaagtata gatggcatta tatcaactcc actggcaacc
     3421 acatataatg caccaatact tttggttgaa aaaaacaatg tacctaatag tgtaaaatca
     3481 gaattaaagc gcctaaaccc taaagatata attataattg gagatgagaa tgctatttct
     3541 aaaactactg ctaatcaaat taaatcaact gtaaatgcta gtcaaacacg tttaaatggt
     3601 tctaatagat atgagacatc tttattgata gcaaaggaaa tagataaaaa tcatgatgtg
     3661 gaaaaagtat acataacaaa tgctaatggc ggagaagtgg atgcacttac tatagcagca
     3721 aaagcaggtc aagacaagca accaattata ttaactgata aagatagtat tacagacaat
     3781 acatataaat ggttaaagag tgaggattta caaaatgctt attttatagg tggtcctcaa
     3841 atgatatcaa caaatgttat aaataaggta aatggaataa ctaaagatag tgttactaat
     3901 aatagagtat acggagcaga tagacacgaa acaaatgcaa acgtaataaa aaaattctat
     3961 acagatgatg agttagaggc tgttttagta gctaaatcag atgtacttgt tgatgcttta
     4021 gcagcaggtc cattggctgc gaacctaaag tctccaatac ttataacacc aaagacgtat
     4081 gtatctgcat accataaaga taatttagaa gctaaatcag ctaataaggt atacaaaata
     4141 ggaggaggat tgacttctaa ggtaatgagc tctatagcat catcattatc taaacacaat
     4201 acgactccaa cagaaccagg aaatagtggg ggcaagacag ttatgattga cccagggcat
     4261 ggtggttcag cacctggaaa ttcatctgga ggaatgattg aaaaagatta caatttaaat
     4321 acttcacttg caacaactga atatttacgt tcaaagggat tcaatgtaat aatgacaaga
     4381 gacacagata agactttatc tcttggaaat agaactgctc tatctaattc attgaaacca
     4441 gatttattta caagtataca ttataatggc tcaactaata aacaaggtca tggtgtagaa
     4501 gtattttata agcttaaaga taaaaatgga gggactacta aaactgtagc taccaatata
     4561 ttaaatagaa ttttagagaa atttaaactt acaaatagag gtataaaaac aagagtactt
     4621 cctagtgatt ctacaaaaga ttatttatac gttttaagaa gtaatgatat gccagctgta
     4681 cttgtagaat gtgcattttt ggataatgaa aatgatatga gtttaataaa ctcatctgca
     4741 aaagtaaaag aaatgggtac acaaataggt aaaggaatag aagattcatt aaaataaatg
     4801 gaaataaaaa tatttaaaaa cttaatgtaa atttatagtt gtaaatttat actaaagtca
     4861 aaaaatgaaa tataagtgta tcaatagtaa gactgtatgt acatttgcac gtgtgaatgc
     4921 aagtatatat acagtctttt tttgtagtta aatgcaataa tttgaatgtg ttttgtaaaa
     4981 tacacaatat gatataatta gataatatac acttaattta attgaggaag gtgattatat
     5041 ggaatattta tttgaaaaaa gtagtttaaa agaatatgga aatgaagctt tagataaata
     5101 tagagaatta ttagataaaa ctggtaataa ttcaaggtgt attcttttgt tagtaccaaa
     5161 caataacaca aagatacgat atgaaaggtc tctaagatta aattatagtg aagaactgaa
     5221 aataacaaca tatataagtt ttgtgaagaa agaattagtt aaatactggc cactaataat
     5281 agaaaaatgt gatgaaataa gtacaaaggt tgtatctcca acatttatat caaatagttt
     5341 aagtgaatat ttaatagtgc aaaaagttaa ggaaaaaagg atacaagaag gatattttga
     5401 agatattaca ggaaccaata aatccatagc aaatagtata atgactaaca taaataagtc
     5461 tgcatttaat ttaattgatt ttgattttat tggagaaaaa atatattcat caaagaaaaa
     5521 taaagatagt atatatagat tttcatacac tcaaatggat gaaattatat catattatat
     5581 aaattcctta ctatcaaatt caattttaga taatgcatta agcatatatt tatacaatca
     5641 atacctactt aatgatgatt tttatttaaa aaacttattt acagaagtga gatatataat
     5701 tgtggatagc cttgaaagct caagtaatgc agaagttgat tttataacag aagcattgga
     5761 taatacatta caaagttata tatattttga ttatagtagg gattattctg tatttaataa
     5821 tgtggatata gactatataa atgagaaaat aatatcaaaa attaagccta aagaagaaaa
     5881 tattaaaaag aaggagatta gaatagaaga tttatactta ttacctgcaa atatagaatt
     5941 aaatcaatca agtcaattat ataatgaaat gttagattta ataagtgaaa aagttatatc
     6001 tcttatagaa gaagatgtat ctccaagaga tatagcaatt atatctccta taaacaatag
     6061 tatattggag catcaaatta gagattcttt gatagaaaaa aatatagatg tttttagtac
     6121 taagaaggat aaaaaagctg tggattatcc atatgggaat gcattggtag tggctacatg
     6181 tatattttat ggatatttag attttataaa agatgatgaa tacttaagct ttttagaaac
     6241 tcttttagaa ataaatagga taaaagcatt taagattttt aatgaaacaa gacatttaag
     6301 tattgataat atagatgaac atagtcagta tcgtgagatt ttacagtata ttgaagaaaa
     6361 aaagaagagt gatataaaaa tacatgaatt tttaactcaa ttttacatag ataaaatgtt
     6421 aaatttaaaa gaaggtaaaa agaatgttgg gttatgtaaa aagataatag ctgaaagtga
     6481 gtcttttagt gaaagtttaa gtctacttgg gatgaaaaaa gaaaagatat ttatagaagc
     6541 tttaaaaagt acaataaacg attattactc tgttgtcgat atagaagagt tgaaatccaa
     6601 agacaaaata gtaataacta ctccttattc atatatatca tcaaatatag atagaagtat
     6661 acaaatatgg gtagatatag gaagtaatgc ttggaatatg aaaatagaaa aagatataag
     6721 taatttaatt gtacttagaa aatcttttga agaaaaaaag atttatacaa atgaaatgga
     6781 agaatattat aaaaaatact atctatacaa tacagtatat aatttgttat taaatgcaaa
     6841 gaaaatctat gcatttaaaa gtgaatatgc gattaatggt tatatccaag agagtatatt
     6901 atatagtata cttttaaaaa tctcacataa aggagataaa aattatgatt aatataaatt
     6961 atagagaaga ccagatacca ataataaatt acgataatgg tactatggca gtaccagcag
     7021 taccaggtgc tggaaaaaca tttataataa caaatttagt agctaaacta ttacttgagc
     7081 agaaacataa gggtggtaaa atattaattc ttacatatat gaatagtgct gtaaataatt
     7141 ttaaaggtag aataagaaaa attcttgaag agaataaaat agatgatact aattcgtatg
     7201 aagtaatgac tatacacagt ttggctgtaa aaataataaa ggaaaagcca gaagttgtaa
     7261 tgttaagtga ggattttaac atagctgacg atttgcaaaa aagtattata ttaaatgaat
     7321 gtataagtag atttagattt gaaggtggag aaagagcttt tagatggttt ttaaaggagc
     7381 aaaaagatga acgctggaaa gaaataactc ttgaagcctg ggaaagaggt ttttttgaat
     7441 ttgcaggaaa tgcaataagt gaacttaagt ataagggaat ttctccagat aaccttgaag
     7501 aaatattgtc aagaggacac aaaggaatgc ttaaaattat tttaccaata tataaattgt
     7561 atgatagaaa attaaagcaa aatggattgc tagattatga tgatatatta attttagcag
     7621 aaaaaacttt aagcttagac gaagggctta gaaagaagtt tcaaactaga tacaaatata
     7681 tatttgaaga tgagtgtcag gactcaaatg aaattcaagg taatataata aaaattatat
     7741 ctagcgaaaa taaaaaccta gttagagttg gagatataaa tcagagtata acaggaacat
     7801 tttcatcatc agaccctaaa ttttttaaag aatttataaa tagtgcagat ttctgttata
     7861 ggatggatat gtcaaatcga agttcaaaag atatacttga tttagcaaac actcttgtca
     7921 agtatgttac aaaggaattt agacagaaag aatgtagaca tgctcttgaa gatatgcaaa
     7981 taaaaactgt gccaaatgga atgggatata aagaaaatcc taatccagaa cattataata
     8041 taaatgtaaa gtggtatgag agttggaaaa ttgaaataga gcaaactgct agatatgtta
     8101 aaggtataaa aaataaatat ccagataaaa gtataggtat attggttcca tttaatgaac
     8161 aagtgacaca agttgctaaa gaacttagag agaataatct aacttttgaa gaattaggac
     8221 caaattcttt gaataaaagg aaggttataa acaacatagc atatattatt gattttatgt
     8281 tacattgtga tgatgtagaa aagttaatag ttgtacttaa taaagttttt atagatactg
     8341 ataatgaaga agaaataaag tattttttag aagaattaaa aaactattct actgaagaaa
     8401 ttatttataa ttttgaattt agtaagttag ataatgattt aatagaattt aaagaagaaa
     8461 gtgacatata tttaagtttt aaaagaggaa tagatattat aaaacagata ttagaatatc
     8521 caataataag gttagacctc ttagttttgt ttattgggaa aaaacttaat ttagaaaaag
     8581 aagataaagc tgtactggat tatatatctt tttatataaa gtatctagta tcagaaaata
     8641 taaatatgga tttagaagat gtatataatt tattgtttga tgtaaagaac agggtgttta
     8701 atcatataat agatgtggta tatgagataa atggatatga accagagccc ggtagtataa
     8761 cactatgtaa ctatcataaa tcaaagggga tggaatggga ttgtgtgttt ttactaggtc
     8821 ttgttgaata taatttccct gacaatatta atcaaaagtt tcagagtgat aaatggtatc
     8881 taaaagaaaa atataaaaac cctatggcaa ttattaaatc agaagttgaa gctatattaa
     8941 aaggaagtat ttcaacagat tatgctcata aaacgaagat tgattctata aatgaaaaga
     9001 ttagactttt atatgtaggg attactagag caaaggaaat gttagtactt tcaggaagtg
     9061 cttatagaga tgaaagtgat atagggaata agagaaaaga gcaaaaacca tgtatatatt
     9121 taagcagact taatcaacat attattgaaa aaaggtcaaa ttaatatata ataagttatt
     9181 aaaagattgc ttgaaatata tctaaaaggg gataaaacaa tatgtacagt aagtaactac
     9241 taaaaaataa agttaagaga gaagtgatat gagtgaacga taaattaaaa tatttttctt
     9301 atagtcaaaa ttcaattaac acatataaat catgtccatt gaaatttaaa tataaatata
     9361 ttgatagaat aaattggaaa aatgatgatg ttggtagtag agagtattat gaaactttaa
     9421 aaacaggaag agattttcat ttgatttgtg aaagatattt tagcaatata cctcttggaa
     9481 tctattttaa tgaggatgat aaaaatagta aaaagttttt aaagtggatg gaaaatataa
     9541 aaaaagttgt tcctattgaa aaaggaaaaa cgtatttgcc agaatatgaa gtaaggatga
     9601 cattaaatgg ggatataata caggcaaagt atgatttagt tgtaattgga aataacaata
     9661 tagaaatatg ggattggaaa accgagtcta aaaagattga ctataaacac gtagaaaata
     9721 gaatacaaac tattgtttat atgtttttag caaaagaagt aatacctaaa gttttaaaaa
     9781 tggacataaa tgttaaagat ataaatatga gatattatca acctgaattt gatgacttac
     9841 caattaccat tttatatgat gaagaaaaac atgaagcaaa tagaaataaa attcaaaatt
     9901 atataagtat gataaaaaac actaattatg aagaacatac atacgaagac aatctataca
     9961 atgatataga aaaggtatat aataaagaag aacgtatgtg ctatagaaat aaaaagcatt
    10021 gcaagtattg tgaatttaat aagttatgta atggcaaaga aatagattat agtattttgg
    10081 aggcggaaat ttatgggact taggtttgta ttaggtagaa gtggaagtgg aaaatccacc
    10141 tatatactag atgaaattaa aaaagaagct caaaaaaatg aaacgacatc aattatactt
    10201 ttagttccag aacagtacac atttgaggct gaaaatagag taagtaaact atttttagga
    10261 aaagaaaaag ataagtatct aagagttagg gtacttagct ttaagacact aagtaatata
    10321 gttttttctc aagtaggagg attgacagat gtaaatataa attctagtgg aaaagcaatg
    10381 atggtatata gagctattga agatgtaagt gaagaattaa atgtgttttc aaaatcaaaa
    10441 tcgcagtctg gttttgtaag ttcaattaca gatatgatat cagaaatgaa acaatacaat
    10501 ataagccctg aaatgttaga aaatatatct ggagaattgg ataatgaaac tttaagttta
    10561 aaattaaaag atataagtaa aatatataat tcttttgaag ggaaattgca tgaaaattat
    10621 gtagatgctc aagatatgtt aacttcattg gcaagtaaaa ttgaattaag cagttattta
    10681 gatggagctt gtgtttatat agatgaattt acaggtttta caccaaatca gtacaatgtc
    10741 ataaagagta tattaaataa gtctaaaagt gttaatatct ctttgactgt agatgatatc
    10801 aactatattg ggtacagtaa atctgatatg ttttctagaa cgaaatttac ttattcaaaa
    10861 ttgactcaat tgtgcaacga agaaggcata aaaatattgc cacaagtaaa tttaaacaca
    10921 ggtgttataa aaagatttga aaaagttaag gaattacagc acttagaaag attttataat
    10981 gcttatccat ataaaatata ttcaaatcca actgaaaata taaagataaa agagtttaac
    11041 aatttatatt cagaggtgga agagatagcc agagaaattg tacatttagt tagagataag
    11101 aatgtcagat atagagatat aaccattgct acaagagatt taaatagata tgatttttta
    11161 gtacactcta tatttaatga atataatata ccaaacttta tagataagaa gagagaggca
    11221 aaaagtaatc caatagttat tttaattatt tcagctttag aaatgaagaa tagacgatat
    11281 gggtatgaaa ctatgtttag gtatttaaaa agtggactta taggaattga taatgatgat
    11341 ataaatttac tagaaaatta tgtgttggct aatgggatta aaggtaaaaa atggtttgat
    11401 gaaaaatggg attatagaat aacccaaagt ctttcaggtc aagaaagtga atttgaactg
    11461 gaattaaaag agaaaataaa tgaaataaaa aatagagtat tagagcctat agtaatttta
    11521 caagaaaaat taagaggaaa aaatagagta aaagaaatct gtagatatat atatgaattt
    11581 ttattagata taaatatgcc agaaactata gaaagtttaa tagtaaattt taaggataaa
    11641 ggggaacttg atgttgcaaa tcaatactca caagtttggg acatagtagt ggacatatta
    11701 gaccaaatgg tggaacttat gggtgatgaa ataatttctt tagaaaaatt tataaaatta
    11761 ataaccttag gttttgatga atatgaatta ggactggttc ctcctagtat agaccaggta
    11821 cttgtaagta gtgtggatag aatgaaaaat ccagacacga aatatttata cctagttgga
    11881 actactgatg gtgtgtttcc gctgattaca aaggatagtg gaatattgag tgataatgat
    11941 agggaaagtc ttggaaacaa aggaatcgaa gttgatattg atagtaagac taggagtttt
    12001 gaagaacagt ttcttgtata taaagctctt acttcgacat ctaagaattt aaccataaca
    12061 tatccaatat cagaccatga aggtaaaacg cttagaccat caataattat atcaagactt
    12121 aagaagatat ttccaaatat agaaaataaa agttatttag tggaagaaaa taaaagcact
    12181 gataaagata tacttaaaaa aataacggtt aaatccccta catttaatga acttataaac
    12241 gtaataaaaa attatgacag tgatggttac aatacagaag agattaatag tatttggtta
    12301 gatatatata ggtactattt aaaagatgaa atctatagtt ctataacaaa aaaagttata
    12361 aaaggtctta gttatactaa tcaagttcat aagattgaag agaaaaaaat aaggtcattg
    12421 tataaaagta attctcttag catatctagg cttgaaaaat atgctgaatg tccatttgct
    12481 tactttatac aatatggtct aaaagcaaaa aagcgtaaag agtattcatt tacaccacca
    12541 gatttaggaa cttttattca taatatttta gataggtttt caaaagaact tttacaagat
    12601 aatttaactt ggagagatat tgatgaaaaa tatatagaat taaagatagg tataatagtt
    12661 gatgaaataa ttttaaagat accaggatat atattaaata gttctgagag gtataaatac
    12721 cttgcatata gattaaagaa tatgttgaca actgctataa ctataattag tcagcaaata
    12781 aaacaaggtt catttgaacc aatagattat gaagttaaat ttggcaacaa tggaaaatat
    12841 ccacctataa aaatggtgtt agaaaatgga caagaagtaa gtttaattgg acaaatagat
    12901 agagttgatg aatttgaaga aggagaaaat aaatatataa gaataataga ttataagtca
    12961 ggcaataagt ccataagtct aacagaaata tattatggtc tacaattaca attacttgtg
    13021 tatttagatg caatccttga aagtgcaaag gatgaagata taaatataaa tccagctgca
    13081 atactatatt gtagaataaa taatccaata gcaaaattta atgaagataa agatgatgaa
    13141 gaaatacaag aagcaatatt aaaagaactt agaatgaagg ggcttgtagt aaaagactct
    13201 cacatagtta aagagatgga taaatcatta atagatggag aaaggaaaaa ctctttagta
    13261 ataccagtag gtcttactaa ggatggaaat gtaggtaaaa gtacttcagc aataagctat
    13321 gaggatttca aactgcttag aaaatatgta aggcatgcta taaaggattt atgtgaagaa
    13381 atgttaagtg gagaaatacg tatagctcca tataaacata aagatggaac atcatgtgac
    13441 ttttgtgatt actctgctat atgtcagttt gattcaacta taaaagataa caagtataaa
    13501 aatttaaata ataagagtaa tgaagagata ataaaaatga tgaaaggaga tgttaattag
    13561 tgagttctcc taaatggaca aaagagcaat tagaagttat agaaagtaga gaatgcaatt
    13621 tattggttgc tgctgctgct ggttcaggaa aaacagccgt tctagttgag cgtataattc
    13681 aaatgataac aagtagagaa aatccaattg atatagataa gttattagtt gttacattta
    13741 ctaatgctgc tgcgtcagaa atgagagaga ggatagggga tgctatagga aaagccttag
    13801 atgaaaaccc agaaaataaa catttacaaa accaacttgt tttattaaat aaatctagta
    13861 ttacaacaat acactcattc tgtttggatg ttataaaatc aaactttcat agaataaact
    13921 tagaccctaa tttcagaata ggagaccaaa ctgaatgtgc tattttgaaa caagaagcaa
    13981 tagaggaagt atttgaagat ttatatgaag aaagagatga ggggttttta aatttagttg
    14041 aaagttatgc agaaagagga ggagataaag aagtacaaga tattatcttg ggaatatatt
    14101 catttgcaat ggcttctcct gaacctaaaa aatggttgat tgactcagca gaacgattta
    14161 atattgatga aaattttgat ttttcacagt ctatttgggc tagagctata ttagacactg
    14221 taaaaataga gattaatggt ctttgtctaa atatggaaag ggcattaaaa gaagtggaat
    14281 caatagaaga attagaaact tttgcagaaa agttatccgt tgaatataaa aaaattgctg
    14341 acatttcaca agcatgtaat aaatcttggg atgaggcata taaaaagatg gcaagtatgt
    14401 cctttgaaaa ttatgtaaaa ggagtaaaaa gaatttcaaa agatgctcct tcgtatataa
    14461 aagaatcaaa agaaaaggca aaaactataa gagataagac taagaagtct ttagaaagta
    14521 tagtaagtgc tacattcaat aaagataatg attctattag agaagaaata aaatatcttt
    14581 ataacatagt taaaccaata tctagtgttg tactgagatt tgaagaggaa tatagcaata
    14641 agaaaagaga aaaaggaata atagatttta acgatataga gcattttgca ttaaatatac
    14701 ttacagatgt agatgaaaaa ggtaatattg taccttctga tatagcagtt ggatgtagaa
    14761 acaagtttta tgaaatattt atagatgaat atcaggatag taatctagtg caggaagtac
    14821 tgcttaaagc tgttgcaaat acagaaactc caaatagatt tatggttggt gatgtaaaac
    14881 agagtatata tagatttaga caagcaaagc cagaattatt tttgcaaaaa tataataact
    14941 ataatgataa aaagggaagc tcccatagaa aaataatgct ttacaaaaac tttagaagta
    15001 gagaagaagt tgtagatgct gtaaattata tatttgaaaa tataatgaat gagaatatag
    15061 gagaaattga atatacagaa aaagaaagac taaatttagg tgcaaatttt aatgtggata
    15121 cagacgagaa atctattatt ggtggagcaa cggagataca cctaatacaa aaagataata
    15181 aacttgatga tgatattata aatgataaag acgatagaat taataacaaa gaaaatgaaa
    15241 tagaggaaga agaaaaatta gataatatac aacttgaggc tagaatggtt ggaaacatta
    15301 tcaaagattt aatgaaagtc aatgaagatg gaaaaattca aaaggtctat gataagggaa
    15361 tagatggata tagacctgtg gaatttagag atatagttat tcttttaaga gctacatctg
    15421 catgggctcc agtgtttgca gatgaattga tgaatatgga tataccaacg tatgctgatg
    15481 ttggagtggg gtattttgat acgattgaaa taaaaacaat attgtcatta cttcaaataa
    15541 tagataatcc aatgcaagat atacctctta tatctgtatt aaaatcacct atatttggat
    15601 ttacaccaga ggatttgatt gatattagag ttcagagtaa agataaaatt ttttatgagg
    15661 ttttaaaaag tacagcagag tatgatggat ttacagattc tcaaaatgaa aatgagtctg
    15721 agtttatacc aagtgaagag tgcataaaca aaagtaaaga ttttttaatt aagctaaagg
    15781 aatttaaaga aaagtcaatg tacatgagta cagatgaatt tatatggtac ttgtacacaa
    15841 ggactggata ctatgcctat gttggagcat tgccaggtgg aagtcaaaga caagctaatt
    15901 taaaagtatt gtttgagaga gcaaaacaat ttgaagaaac cagtcttaaa ggaatattca
    15961 actttgtaaa ttttatagag aaattaaaaa aatctagttc tgatatggga agtgcaaaaa
    16021 cacttggtga gaatgcaaat gttgttagaa taatgagtat tcataaaagt aaaggtctag
    16081 aatttccagt agtcatatgc tctgcaatgg gtaaaaattt caatactcaa gattttaaaa
    16141 agagtatttt atatcatcat aatttagggt atggacctca atttgtagac tatgagagaa
    16201 ggatttcttt tccaagtata gccaaagaag cattaaaaag caaaataaat atagaaaatt
    16261 tatcagaaga gatgagagtt ttatatgttg catttacaag agctaaagaa aaattgatta
    16321 taactggttc tactagaaat atacaagata gtataaagag atggtcaaat ggaattgaaa
    16381 gtttagatac aatatcacaa tacgagatat taaaaggtaa aaatttttta gattggataa
    16441 tgccttgtgt attaagacat agggatttat ctaatctact agaagaagta ggattagacg
    16501 cagtttttaa tgttgaacat aattcaaaat ggtatggaaa gttgtggaat aagaatgata
    16561 ttttagtgga aaagaagagt gatgaagaaa aagaaagtat tgaagaaatt cttgaaaaaa
    16621 tagatgtaaa taatcctgac agtgattatt acggtgaaat agaagaaaaa ttaaactata
    16681 tatatccata tgaatttagt acaagaaaac ctgctactat atctgttaca gaaataaaaa
    16741 agatacaaaa taattatgaa gaagagttaa taaatactat atttgaacaa aaagtaatat
    16801 taaaaaagcc tctatttatt caaaatgaag aagaaagaga aaaaataagt ggaacagaaa
    16861 gaggtacaat agtccattta gttatggagg ttttagactt aaaaaatgta agtagtgtaa
    16921 atgatataaa atcacaaata agaggttttg tttcaaaagg tataataaca gaaaaacaag
    16981 ctagtatagt aaatccatat aaaatatata aattttttgc ctctaacata ggaaaaagaa
    17041 tgttgaatgc tgaaattata aatagagaaa aatcaattta tgcacaagta aatatgaaag
    17101 acatatatat ttatgaaaag cttataaaca atgacgataa aaaactttat gataatgaaa
    17161 gtgtcatgtt aagaggtata gttgatgctt attttgaaga agataatcaa atagttttgg
    17221 tggattataa aacagatttt gtaaatgagg aaaatataaa tcaaataata gagaaatata
    17281 aaaaacagtt ggatttatat gcagatatta ttgaaacttt aactggtaag tcagtaaaag
    17341 agaaatgtat atatttattt ggagttgatg aagctgtttg ttattagttt ttatatataa
    17401 attaaaccta aaagatagag gaaggggaaa aaatgagatt tattcataca tcagattggc
    17461 atctagggaa gtccctagaa ggccattcta gaattgagga acaagcaaag ttttgtgagg
    17521 aatttataaa aatagtagaa tcaaatgaaa tagatatggt tataattgct ggagatgtat
    17581 atgatacttc taatccccca gcacaagcag aaaaactatt ttatcagact gtatcaagac
    17641 ttgccaataa tggtcagagg tgtgtattaa taatttcagg aaatcatgat aatccagaga
    17701 gactctctgc aataacacct ctagcacatg aacaggggat acttatatat ggttatccat
    17761 taagtgctac gatagaagct aagtataaag ggtttgaaat aacatatgca actcagggat
    17821 gtacaaaact gaatataaat ggagaaaata ttgtggttgc tacattacct tatccaagtg
    17881 aaaaaagatt aaatgaagtt ttttcaagtg aagatgagtt tgagaaacaa aaaaattatt
    17941 ctgaaaaggt aggagatata tttagaagtt tagaagaaaa ttttagaagt gatactataa
    18001 atatagccgt atcacatata tttgtaatag gtggagagag tactgaatca gaaagaccta
    18061 tacagttagg tggaagtttt ttagttgaaa gaaaagattt gccagaaaag gctcaatata
    18121 ctgcactagg tcatttacat aaacaacaaa aagcttccga acgtttaaat gcatactatt
    18181 ctggttctcc acttcaatat agcaaggatg agagagcgta taccaaaggg gcatacatag
    18241 tggatataaa agctggtgaa aaaccaataa ttgaagatgt atattttaat aattacaaac
    18301 ctatagaagt atttaaatgt aatggaatag aagaagcact agatatatgt gagcaaaatc
    18361 aagatagaga gatttggtca tattttgaaa taaatactga tgagataata tcacagaatg
    18421 aaattaaaaa aatgaaggaa ctattaaaag acataataga aataaaacca ataataacat
    18481 cttgttatga acaagaaagt gtagatataa aagaaaagtc tatggcagag ttgtttagag
    18541 agttctattc ttttagtaaa ggtgtagagc caaaaggaga acttatggat ttatttctag
    18601 atataataag tgaagaaggt gaatcagcag atgagaccaa ttagattaga acttacaggg
    18661 cttaatagct atattgataa acaagttata gactttgaaa aattaattga aagaggtttg
    18721 tttgggatat ttggaactac tggaagtgga aaatctacaa tcttagatgc aataactatt
    18781 gctatgtatg gaaatatatc cagaaataca aaagaatata taaacagtgt gtgcgataag
    18841 gcaattatat catacgagtt tgagatagga agcaaaaaca ctagaagaag atacattgta
    18901 gatagaacca ttgctagaag caaaacagga acaaagacct ctaatgctag attaatagaa
    18961 gtattgaatg acaataccca aaatgtttta gctgataaag ttgtggaagt aaatgaaaaa
    19021 gtagcacagg ttgtaggatt aacagcaaat gattttacta ggtcagtagt tttaccacaa
    19081 gacaaattta atgagtttct aagattatca cgtgcagata gaagagacat gttagagaga
    19141 atttttaatc ttgagaaata tggaagaagt cttggagaaa aagtgaaaaa gagaaaaaat
    19201 atacagttac aaaatttaaa agatttgaaa tcaaaattaa gtcaatatga tggaattaca
    19261 gaggaagtat acaacaatat aaaccaagaa ttaatagagt taaaaaattt ggaaaaagat
    19321 aaggataatg ctttagacct agctcaaaaa tcttatgaag aaagtaaaac agtatatgaa
    19381 gaacaattaa agttagaaaa aaatgaactt agaaaaaatg agcttgattt aaaaaatagt
    19441 gagataaaag aaaagattgc tcttgtagaa aatgatagta acgctaaaaa ggtagaccca
    19501 tacataagtt cagttcaaaa tctagaaaaa aagatagatg aagatagctt cgcagtaagt
    19561 aatttagaaa agaaattagc aatattaaat caggagcttg aagttacaaa aaatagatat
    19621 gaaaaaataa acaaaattaa gaatgaagaa gtccctaaat tgagtgaaga aaaaataaga
    19681 ttgcaacaag caataaaatt agaagaagag ttagttttat tagatagaga gttaaaagag
    19741 ctaaaagaaa gtggaattaa tttaaataag actaaagttg aattagaaaa agtaaagcag
    19801 gtgtcagagt ccagaaaaga tgcagttact aaatctataa aagaagttga aggaaagatt
    19861 gataaagtaa atataagtgc agaattaaaa caaaaaatat ttctagcata tgaatacgag
    19921 aaagattaca acaacgtatt ggaagaaaaa aatcaaaagc taaataaact agaagaaata
    19981 ctcaaagaga cagaaaatat aaatttaaaa gttagatata ttgataaaga taagaatgat
    20041 gtaaatcgta atttagaaaa tttatcactt cacttggatg ttcttcttaa aaaatgtcct
    20101 ggtaagagtg ctgatttact tttaaaatct gaatatgtta ctgaattaag aaataaagca
    20161 aataatacta aagaaaatga aattaagaaa agcagtatac aagatgaatt aaagataata
    20221 cttgaaagta agtttaatac tgaaagggaa gtaaatttac taaatgaaaa acttgaaaat
    20281 aatagaaaga acagagatga cttagaaaaa gaattagaag aactaaagta tttaaattta
    20341 gcctctgaac ttagacgtga attaaaagaa aatatgcctt gtccagtttg tgggtcaaaa
    20401 catcatgaaa atcatgatat aactaagtat gatgaaaata tatcttttgt caaagaaaaa
    20461 ctggagaaat tagaaaaaga aaaaataagt ataagaaaca atatagagga attaaatgct
    20521 aaagtaagtg ggtatttatc tatagaaaaa atgaagacta aagaacttga agatgtaaaa
    20581 ggaaaactag gggggattcc atctagtcaa ttattaaaaa aacttgatga agaacaaaga
    20641 aaacttgcat tgttaaaatc taatattcag gagtgggaga aagaaaaaga gagtactgaa
    20701 aataaagtaa cattagctaa agaagaaaaa aataaaattg aaaaagaaga attaaaaata
    20761 agagagagtc tgaataatta taagaaatta acaagagatt taaacataga aatagaaggt
    20821 ttagaaaata aatgtaaaaa acttaaacag gaatacctag gattaaagac gattactaaa
    20881 gtctctaatc tattgtcaaa agtaaaagaa ataagagaga atgaaaaaga attagaatta
    20941 ctaagtactg ggcattcaaa tttattaaaa aatagagact cactagatat taaaataaga
    21001 gaaaatgaga gtaaactaca tgaaatagag atagaattaa taaaggctag agaattatat
    21061 gtagaaaaga aattgagtag agataataag tataaagaag ttattagcat aacgaaaggt
    21121 gatttagcta aaaatctttt acataatgtt gaagaaaata tatgtaagat attagaacaa
    21181 gaagaatcaa gtaagaaaaa attggaagag caaagattag aatatgaaaa aaacattgct
    21241 gaaaagcaca atatagatgg tagacttaaa actgctaagg aacaatataa agaccaaaaa
    21301 tcaacactaa ataaactatt agcagaaaat aaatttgaaa gtatatatgc agtaaaaaga
    21361 gctttattag atactgatgc agtaaaaaaa ttgattgaag aaatactaga gcatgaagaa
    21421 gaacaaaagt tattatcatt taaaataaaa tcatcaaaag aaaaattaaa tggaagaagc
    21481 ataaagaagg agtactttga acagttaaaa gatgaaattt ataatttgaa agtagaaata
    21541 ggaaatatat ccaaagatat tggagctaat caaaatcagt taataacatt aaaagactct
    21601 ttagataaga taaatgattt taataaacaa cttaaggtag ttgaacacaa tgtggactta
    21661 ttggaagaat tagataaatg tgtacaagga aataaatttg tagaatatgt ttctacaaac
    21721 caattaaaat atatagcgtt ggaagcatca aaacgattgg atggaataac aaaaggaaga
    21781 tatgcacttg aaattgactc aacactaaac tttgtaatga gagataactt taatggagga
    21841 gaaagaagaa gcattgatac tctatctggt ggagagacat tcttgacatc tctatcatta
    21901 gcacttgctc tttcatctca aatacaatta aagggaagtg caccattaga atttttcttt
    21961 ttagatgaag gttttggttc attagatagt gaacttttag aaattgttat agaatcattg
    22021 gaaagattgc atagtaataa tctaagtgtg ggtataataa gtcatgtcga ggaacttaaa
    22081 aatagagttc ctgttaagct attagtttca tctagtgagg ctggaatagg ttcaaaagta
    22141 aaaattgaat atagttagaa aatatatgtt aattgaatat attatctaat atttccaaat
    22201 aatatgtaat tgtgatataa taaaaactaa tattaaagtt atttataatt aatattaata
    22261 taaaaacatg taagtaggag tgtggtaagt gttggaatcg cccaataatt tgattcagat
    22321 tattttttta atagtattac ttatagggtc tgcatttttc tcagcatctg aaacggcttt
    22381 aatgtcgttg agtaaaatca gaatcagata tatgcaagat gaaggagtta aaggagctaa
    22441 gttagtaagt tcattaatag aaaatccaaa taagctttta agttctatat tggttggaaa
    22501 taacgtcgta aatatagcag caacatccat atcaacatct ttgtttatag gattaatggg
    22561 agaaaaagga gtggcacttg ctacagctgt tatgacagta ttagtattaa tatttggtga
    22621 gattactcca aaaacaatag cagcaaataa ttcagaaaag gtttctctat tagtatctaa
    22681 acctataaaa gcaattatat ttatattaag accaatagta tggatattta atataattac
    22741 aaatattata tttaaattat ttggtataac aaataaagga gctaaatcat tcataactga
    22801 agaagagcta aaaactatgg taaatgtaag tcatgaagaa ggcgtccttg aaatggaaga
    22861 gagagaaatc ataaataatg tttttgaatt tggagatatg caagctaaaa atgcaatggt
    22921 acaaagaata gatatggtag ctatagatat ggaagatagt tatgatgaga taatacaggt
    22981 atttaaaact gaaaaattaa gcagaatgcc tgtgtatgaa gaaactatag atgatattgt
    23041 aggaatactt aatataaaag atattatatt tttatctgat gaagaaatag agtcatttga
    23101 tattaaaaat tatatgagag aacctttttt cacgtatgag ttcaaaaaga taacacaact
    23161 tcttgaagag atgaaacttg aaaaaagtca gatggctata gttgtagatg agtatggtgg
    23221 aacatctgga cttttaacta tagaagattt agtagaagtc attgttggag atatagagga
    23281 tgaatatgat gaagaagagg atgaaataca agttataaaa gaagatgagt atattgtaga
    23341 tggaagtact aaaattggtg atgttaatga acttattggt gtaaacttgg aatctgagga
    23401 gtttgattcc ataggaggtt ttataattgg acacctaagc agattgccag aagaaaatga
    23461 agtaattgag gttgataata taagattttg tatagaaagt atagaaaaaa atagaattaa
    23521 aaaaataaga atatatacat aatgtaataa tgttcttaaa tttattagaa gtgtataaat
    23581 ataaaagata tcttttatat ttatacactt ctttttattt tgtattcaaa taaaactagt
    23641 atgaaatgga caaaataaaa atataaatta gaagaaagga gatttttaag atgagcatac
    23701 tagaaaataa ttttttatac aaattaaaaa acaacactat ctttttttgt atatatacat
    23761 tgttatttat ttttatttac aaagcgtttc catacatagc tccatttttt ctaggtgcta
    23821 taatagcact tatgattaat cctatatcac aaaagttaga aaataagttt cgtataaata
    23881 aaggtatctc tactcttgtt ctaagttttc ttgcagtggc tatagtcagt acagttacta
    23941 caataatagt aataaattca atgaaagaat taatgggatt tttaaataat atatctgcta
    24001 atcctgagga tataagtaat actattatgt attttttaaa taaaataaat gactttatga
    24061 aatcatttca agaaatagcc aattttgatg tagaacaatt agtaaacaaa ttcagtggtg
    24121 aagtaatgca aattacaaaa aatcttctta ctagtatact tggattggct acttctattc
    24181 catatattat aatatttata attaccttgt ttattgctac gtactttata gcaaaagacc
    24241 ttgataagat tgaaaatagt ttttataata tgtttactgt tgatgttaga aagaaggtaa
    24301 aaaacgtaaa gaaagaggca gggctttcac ttgttggata tataagggcc tatacaatac
    24361 ttatggctat aacatttttt gcaatctggg gaagttttgc tttatttgga ttgaaatacg
    24421 gtctaatagt agggtttgta ggagctttaa tggatttaat tccattccta ggtataataa
    24481 caatatatct tccagtaatt gtttattatt tccttataaa aaattatttt atagctataa
    24541 gtatgactgt gatatttttt gttttgtctt tgattagaga aatacttgaa cctaaactag
    24601 tatcagtaaa tgttggacta aatccattag ctaccctagc agcgattttt attggaatcc
    24661 aagtaaaagg aataattgga gtaatttttt gtttgggttt ggtatgtatg catgatatac
    24721 ttaagaaggt ggatattttt taattataat aattgataga ttttttaaaa ataaaatttt
    24781 attcacaggt aaataaaaaa atattaatct tattaagaaa gagggtatat acgtgaaaca
    24841 aaaagtagtg gaaagatttt taaaatatgt atcatttgat acaacatcta attctcagtg
    24901 tgagaactgt ccatcttcag aaggtcaaag agttttagct aagtatatag ttgaagagct
    24961 aaaaacaatg ggtgttgatg atgtgagttt agatgaaaat agttatataa tggctacttt
    25021 aaaaggaaat actgatggtg tggatactat aggattcata tcacatttag atacaattga
    25081 agatgttagt ggaaaagata ttaaaccaag aataatagaa aattatgatg gaaaagacat
    25141 agttttaaat gaagcgttaa atgttattac gtatgtaaag gattctccag agttggaaga
    25201 atttaaaggt gacgatttaa tagtcactga tggaacaact ctacttgggt ctgatgataa
    25261 agctggtata gctgagatag tgactgcaat agagtatcta ataaatcatc cagaaattaa
    25321 gcatggagat ataaaaatag gatttacacc agatgaagaa attggtagag gtgctgattt
    25381 atttgatgtt gaaaaatttg gagcaaaata tgcatatact ttagatggag gaatagttgg
    25441 agaacttgaa tgtgaaaatt ttaatgctgc aaatgcaacc attactatac atggtagaaa
    25501 tgttcatcca ggttcagcta aaaataaaat ggtaaatgct atacatatag ctgctgaaat
    25561 atcagaaatg ttcccagcag atgaaagacc agaaacaaca gaaggatatg aaggattttg
    25621 gcatttaaat tctataggtg gaaatgtcga gaatgtaagt atggcttata ttattagaga
    25681 ccattgtaaa gaaaaatttg aaaatagaaa atctattatg atagagaata tagaaaaaat
    25741 aaataagaaa tacgataata gagtggaatt ggatttaaaa gactcttatt acaatatgaa
    25801 agaaaagatt gagccagtta tgtttatagt tgatatagct aaagaagcta tggaggaatt
    25861 aggtataaaa ccacgtttag ttcctgtaag aggtggtact gatggagcaa gattatcttt
    25921 taatggatta ccttgtccaa atatatttac tggtggatta aacttccatg ggaaaaatga
    25981 gtgtatacca gttagttcta tggagaaagc tactaaacta attgtaagaa tagctgaaaa
    26041 atatgctgaa agagtttagt taaaaagtat ttaaaaatta agcatttaaa gttataatag
    26101 ctatatcatt ctttataagt caagctgatt ataagcttgt gttgtactta aaatggtata
    26161 gctatttttg taattatttt tataaaagat aattattttt agtttagggt atcaataaaa
    26221 agtatgaata tcaataggta ttaacattta aaagggggaa ttgtattgaa aattaaatct
    26281 aaaaaaataa tagcatttgc actttcactt gggattatgt tgccaagttg tgtatatgca
    26341 gacactatta gtaaaaatca aaatgcacag gtagatatga acaattatct tcaaggtaga
    26401 aaatctccaa aggaggaaaa acttattggt gaagatagat ttgatacagc gataaaaata
    26461 tctcaaagtg gttggaataa tggttcagaa agggtgtttc tagttaactc aaattcattg
    26521 ccagatgcat tggcttcaac tcctttggca agtaaattgg atgcaccaat actgcttaca
    26581 aataaaaata gtataccata taatgtaaca gaggaaataa aaaggcttaa tccagcagaa
    26641 gttattttaa ttggaagtga aggagctatt tcatcaagtg taaaagaatc tcttgaagat
    26701 atggaagtat tagtcagtag aatcggtgga gcagatagag aagaaacatc tttacttctt
    26761 actagacagc tagatgatac tggtgattta ggtgtctcaa aagtggcagt ggttaatggg
    26821 tataatggac tggcagatgc aacttcaata tcaagcccag ctgcaagtga taatacagct
    26881 ataatatata caggtaagga tagcataaga agtgaggcaa agaattttat tacacaaaac
    26941 tcaactaaga catacatagt aggtggggag tacagtataa gtaaaaaact tgaaggacag
    27001 ttagtaaact cagaaagact agcagggaca gatagaaaag atactaatgc gaaagtttta
    27061 gaaaagtttt atggaaaatc aagtaaggtt aataatatgt attttgcaaa agatggaagt
    27121 ggtagagaag ctgatttagt tgatgggctt gctgctggag tattagcatc aaaaacaaag
    27181 tcacctgtag tacttgcaag tggaagttta agtagtgcac agaaaagttt tataaaaaaa
    27241 gtaaaggctg ataaatttgt acaagttggt ggaggtaaaa atagtaaacc atatgcagaa
    27301 gcattagcct tacagtaata ttgtgtaaat caaaattaat atataataat agcaagtaaa
    27361 ccaagttgca cttcaatata tataataaaa taagtaaact aagctatgtt tcaatataat
    27421 atataataaa ataagtaaac taagttatat ttcagtataa tatataatga aataagtaaa
    27481 ctaagttata tttcaatata gcatataata aaacaagtaa accagattct atttaattag
    27541 aagtttaagg tattgtactt aaacttctaa ttgagataga aataaaatgg tttacttgtt
    27601 tttattatga
//
LOCUS       DAYATN010000041        27047 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.041, whole genome shotgun sequence.
ACCESSION   DAYATN010000041 DAYATN010000000
VERSION     DAYATN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.041"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            611..1366
                     /locus_tag="ACGI8A_001677"
     CDS             611..1366
                     /locus_tag="ACGI8A_001677"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFH0179676.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(1509..1937)
                     /locus_tag="ACGI8A_001678"
     CDS             complement(1509..1937)
                     /locus_tag="ACGI8A_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFH0179677.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(2358..2558)
                     /locus_tag="ACGI8A_001679"
     CDS             complement(2358..2558)
                     /locus_tag="ACGI8A_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFH0179678.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(2741..3895)
                     /locus_tag="ACGI8A_001680"
     CDS             complement(2741..3895)
                     /locus_tag="ACGI8A_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HFH0179679.1"
                     /translation="MKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNMEEKVM
                     ESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYENEVKK
                     MKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQKDKENA
                     FRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGVELQLT
                     DEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGRFKKNS
                     IWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFVGYEKK
                     GRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            4146..4967
                     /locus_tag="ACGI8A_001681"
     CDS             4146..4967
                     /locus_tag="ACGI8A_001681"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HFH0179680.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(5043..6431)
                     /locus_tag="ACGI8A_001682"
     CDS             complement(5043..6431)
                     /locus_tag="ACGI8A_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HFH0179681.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(6566..7324)
                     /locus_tag="ACGI8A_001683"
     CDS             complement(6566..7324)
                     /locus_tag="ACGI8A_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HFH0179682.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(7326..8042)
                     /locus_tag="ACGI8A_001684"
     CDS             complement(7326..8042)
                     /locus_tag="ACGI8A_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFH0179683.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(8044..8751)
                     /locus_tag="ACGI8A_001685"
     CDS             complement(8044..8751)
                     /locus_tag="ACGI8A_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HFH0179684.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(8867..9403)
                     /locus_tag="ACGI8A_001686"
     CDS             complement(8867..9403)
                     /locus_tag="ACGI8A_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0179685.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(9505..10575)
                     /locus_tag="ACGI8A_001687"
     CDS             complement(9505..10575)
                     /locus_tag="ACGI8A_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFH0179686.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(10568..11626)
                     /locus_tag="ACGI8A_001688"
     CDS             complement(10568..11626)
                     /locus_tag="ACGI8A_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFH0179687.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(11613..11945)
                     /locus_tag="ACGI8A_001689"
     CDS             complement(11613..11945)
                     /locus_tag="ACGI8A_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFH0179688.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            12144..13265
                     /locus_tag="ACGI8A_001690"
     CDS             12144..13265
                     /locus_tag="ACGI8A_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0179689.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(13393..15291)
                     /locus_tag="ACGI8A_001691"
     CDS             complement(13393..15291)
                     /locus_tag="ACGI8A_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HFH0179690.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            15606..16382
                     /locus_tag="ACGI8A_001692"
     CDS             15606..16382
                     /locus_tag="ACGI8A_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFH0179691.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
     gene            complement(16474..17226)
                     /locus_tag="ACGI8A_001693"
     CDS             complement(16474..17226)
                     /locus_tag="ACGI8A_001693"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFH0179692.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(17387..18337)
                     /gene="galT"
                     /locus_tag="ACGI8A_001694"
     CDS             complement(17387..18337)
                     /gene="galT"
                     /locus_tag="ACGI8A_001694"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFH0179693.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            18722..19918
                     /locus_tag="ACGI8A_001695"
     CDS             18722..19918
                     /locus_tag="ACGI8A_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFH0179694.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(20253..20705)
                     /locus_tag="ACGI8A_001696"
     CDS             complement(20253..20705)
                     /locus_tag="ACGI8A_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0179695.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(21016..22461)
                     /gene="purB"
                     /locus_tag="ACGI8A_001697"
     CDS             complement(21016..22461)
                     /gene="purB"
                     /locus_tag="ACGI8A_001697"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFH0179696.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(22605..23513)
                     /locus_tag="ACGI8A_001698"
     CDS             complement(22605..23513)
                     /locus_tag="ACGI8A_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0179697.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            23770..24594
                     /locus_tag="ACGI8A_001699"
     CDS             23770..24594
                     /locus_tag="ACGI8A_001699"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFH0179698.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            24634..25164
                     /gene="lepB"
                     /locus_tag="ACGI8A_001700"
     CDS             24634..25164
                     /gene="lepB"
                     /locus_tag="ACGI8A_001700"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFH0179699.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(25246..27027)
                     /locus_tag="ACGI8A_001701"
     CDS             complement(25246..27027)
                     /locus_tag="ACGI8A_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFH0179700.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         9076 a         4250 c         3119 g        10602 t
ORIGIN      
        1 ataataatga tatcatatta tttaaaattc taaaaacaaa tatatgcttt tgttaagaaa
       61 ggttgtttga tgaatgtata aagaaaagtt gtttgataat tattttaagt tcttagcact
      121 attattttgg ccaattatgt ggtataagtg gatagtaatt tcaaatggta ctcttgaaaa
      181 tatgttattt accatatatg caatcgtcgc aattgttttc atcattttat attcggtgtt
      241 tatgataaaa tataaagaca ttacacaaat agatttcttt tacaggataa gtactctact
      301 tgcttttata tttacacttt ttagcttttt aatataccca aagagtttat ttttcttgta
      361 tttaaagatt atttttactg gaatatacct ttattattca atagtaaaaa ctcttaaatt
      421 taaagatgac gaaggtgtag tgggtattat gagctcactt ttattaattg taataacttt
      481 gttctactaa atttaattct atttgactta atatggcttt aaatattaga catctacatt
      541 attttctgat attatttaag atggtagtct tatattaata atgaaataca ctatgaagga
      601 gttaactatt atggaaaaaa aagttttagc aacagttgga gaaaaagaga taactaatat
      661 tgatattgaa aatgcattaa aatcattaga cccttatcaa gctatgcaat ttaaaactga
      721 agaaggaaaa aaacacttat taaatgattt agtgaatcaa gaattattct tcttagatgc
      781 taaagaagaa aaattagatg aagaagaaat atttaaatta gaaatgaaaa agatagaaga
      841 aaatgtatta aagcaatttg ctataaataa agttttatct agtgttaatg taacagaaga
      901 tgaaaaagta aaattctttg aagctaataa gagctctttc tctaaaccag aaagtgcaac
      961 tgctaaacat atattagtag atagtgatga aaaagctaaa gaaatacttg ctcaaataaa
     1021 atcagaagaa atatcttttg aagatgcagc tcttaaacat tcttcttgcc catctaaaga
     1081 tatgggtggt gatttaggaa cattcggtag aggacaaatg gttcctgaat ttgaagaagc
     1141 tgtgttctct atggctaaag gtgaagtaag tgaaccagtt aaaactcaat ttggatacca
     1201 tataataaaa ttagaagacc ttcaagaaag tacagagtct acatttgatg aagttaaggc
     1261 tgaagttgaa aaaagtttat tatatcaaaa acaaaatgaa gtttatggaa ataagataaa
     1321 tgctcttaat gctaagtacg gaaaccttgt aaaatacaac gattaatcat aaggtcatat
     1381 ataaaaaagc tgtcttttca caatatttat tagaaaagac agctttttat attaaagttt
     1441 aaatattcag tttcatatta tcattttttt attgtaaaga actcagctta aaagtttaaa
     1501 taagattttt aattatcatt agctaaattt ttatatccac gtattatctc ataacagtaa
     1561 cctattacat ctcttctttt tattatccca ataaatgtgt cttggtcatc tataacagga
     1621 atgaaatttt gatttaaaga ttttataact aaatcttcaa tatctgcgtt gatggaaaca
     1681 ggagaattat ccatttttct atttatatct gtcactggaa catcttccaa tccctttaag
     1741 tccagattta aatcattttt taatgtccat aaaaaatcac cttctgttat agttccaaca
     1801 tatttaccat ccttacttat tattggaatt gcagagtatt tgtgatattc cattttttct
     1861 agggcttgcc ttatagtgta gtcttcatat atatatgcta cttcactttt tggtgttaga
     1921 aagaataata tgttcattac actacccacc gttactgaaa acttataaat attactcaat
     1981 atttataatg ttactttttt gcagtaactt tctcctttct tattttctaa gtattcttaa
     2041 tactatacgc tgtaacaagc gtttttcctt tgctcatttt aatcttatta tgctttatta
     2101 atatttttat atggtctata agtatcactt attctctttt tagaaaattt actaaaaatt
     2161 aattgactta aacttagcca taataatttt actctacact attaatttta acatatttat
     2221 ctcattatta atgattttat tttaataatt ttaatttatt ttgctattaa ttttatttac
     2281 aaaaactgaa aaaaccgcta aatgattagc ggcttatctc tcatattact agaacactaa
     2341 tatatatgaa tttttaatta taatctaact acgttagcag cttgaggacc tttagctcca
     2401 tctactactt cgaactcaac gctttgtcct tcttctaatg ttttgtatcc atctccagtt
     2461 atagctgaga aatgtacgaa aacatctcct tcaccttcta cagatatgaa tccaaatcct
     2521 ttttcgttgt taaaccattt aactacacca gttttcatta ataaatcctc ctaaaaaaac
     2581 acaaatattt agctactctt ttctgtaacc ttattactac tctatcatat atagctatgt
     2641 ttgtcaatta ttattatttt tatacattaa taatcaattt ttttacttta taattttaaa
     2701 tgattattat cttttacact ttatttttct ttataatttt ttaataattt atttttatta
     2761 tatggaatct tatggactta tcactccagc ctaaatccca tggcaccaac attctatcag
     2821 tattatggca agttaccaaa ggatatcctc ttgagtctaa tcctgtcact gtcgaaacat
     2881 gtgttattct gcctttcttc tcataaccaa caaaatcccc tggacgtaaa ttataagctt
     2941 ctttgtatac ttcttcatag gaacctttag caattaatga acctcttcca ctatacagtg
     3001 cataattttt aaaagcttgt gcatttaccc atgcttttgt acctgctctc tcgctataat
     3061 tccaaattga gttctttttg aaccttccac tttcaaacat tatctgtgat gcaaagtttg
     3121 cacaatctcc accttctgga ttataatccc tatagtttgg attaaatttc ataccatgtt
     3181 cttcatcagc tgcggctcca cagtatttat gtgcataatc tatagctttt ttttgttcat
     3241 ctgtaagctg taactctaca ccatcttgtt gaccaatata atttcttata tcatcagatt
     3301 ttatattttc taaatttaaa gagtctgcaa aagggtctgt ataccactct ttagttatga
     3361 catattgatt atctataatt tttacattta gataatgata tgtacctatt ctaaaggcat
     3421 tttctttgtc cttttgattt tcatatgaat acttatattc agtggatact gaacagatta
     3481 taccatagag gtctttttct ttttccttta tttttttaat cttaaccctt gtctttatgt
     3541 ctttaaattt tacaccttgt ttactagacc agttttctaa atatttcatc ttttttactt
     3601 cattttcata agcccacaga ccaaattttt tatccgtttc ataaagacct tttaaaatat
     3661 tttcattttt ctctaatatt gccttatttc tgtagtcaaa taagttttca agcaatagtt
     3721 tatattggtc ttttacatca tcaccttcaa catttgcaga ttccatgact ttttcttcca
     3781 tatttttcat acttaaatca ttgattaaaa atacacctgc tgtagtagct aaagacaaaa
     3841 atacaaatgc cattatacct ttgatgtact tattcttatt tttcagtatt ttcaaagcta
     3901 cacctcccaa atagtacacc ttacaaatca aattacaaag aatattctgt cacatatagt
     3961 aattttcata tttaataatt ctctatttta ttatgtccaa tattaaaaat taattgtatc
     4021 aatacactgt tttttttaca attttttatt ttataatcta cttttttctt ttttttatag
     4081 taatatatat gtatataaat attttggtat cattataatt aatacatact aatggagggt
     4141 tttttatgca aaaaaaagtc gcagcaatta atgacttatc tggaatagga aaatgctcat
     4201 tatcggttgc tatacctata ctttcagcat taaaggttca atgttgtcca tttccaacgg
     4261 ccatactttc aagtcaaact ggttatcctg aatttacttt cttagacttt actgatgaaa
     4321 tggtaaaata ttcaaatgtt tggaaaaatt taaaagtcaa ttttgatagt atatatagtg
     4381 gttttttggg ttcaaagcat caaattgaga tagtggctaa ttttataaac gattatccca
     4441 atgcttttat cgtagttgac cctgtgatgg gtgataatgg agttatgtat ccaatattca
     4501 cagaagaaat gagacaggaa attaaagaat tagttaaaca ttcagattta actactccta
     4561 atttaacaga agcttgtttc ttaactggaa atgattatac caaaagcgat tataatagag
     4621 atgagcttat atatattgca aaatctgtat ctgatttggg acctagcaaa gtcgttatta
     4681 ctggtatatt agaagatgat aatatattaa atttggctta caatagagat aatgaccatg
     4741 tattctttac atcagtcaaa tataataatt gttcttacag tggaactgga gatatattca
     4801 catctatact ttgtggtatg ttagtaaata aacatgattt gggagtagcg gttaatactg
     4861 caacagattt tatatataaa actataaatt acacatctca atttgatact gatagaaatg
     4921 atggagttat gtttgaaaac tttttatcag atttaaccaa tatataaaat caaaaaaact
     4981 atccatttta taaataccta taaatcataa aatttaggta tttacaaaat ggatattatt
     5041 tactaaacta ttggtatttc aattgttacc atagcaccac cagttttcac agaattttca
     5101 atacataaag tcccattatg tttttgtaca atattattta caatatagag tcccattcca
     5161 taatgagatt tagaatttct actcaaatct cctgtataaa actgttctgt agcttttgac
     5221 aaatctgctt tagaaaaacc acttccacaa tctgtaacta caaattttaa gtatttatta
     5281 tgtactgata cacttatgta tagtttactc tggcttggtg aataatctat agcatttgaa
     5341 attatattca taatagctct gaataacagt tctctatcta tagttatatt tttaggcaaa
     5401 ttatcttgtt tataaaactc cacatctaag tttttagtac tagctaatgc ttctatttgc
     5461 actaaaacat cttttataaa ctcactcata ctgattgctt ctttatttaa tatatagcca
     5521 ttctctgttt ttgatatatc tattaaagta ttaatatatt tttctatttg agtgacaccc
     5581 tttgagatat attccatgta ttctaataac tcaggatttt catttatttc aattaataag
     5641 tcagtatttc catgtataat tgttagtggt gtttttatat catgtgccag tgcagatatc
     5701 tgttcctttc tattttcttc tagcatccat tgtttttcta aagaatcttt taacgccaac
     5761 ttcattttat ccatagaaaa aaggacatta ttaatttcat aaattttact agattctaca
     5821 acaaaatcta aatcctgttg ttctattttt tctgtagtat tttttaaaag ctccatttca
     5881 atattaaatt tcttgccaaa tacttttgat aatataacaa tttcaataaa aaatattata
     5941 ctaaaaataa tcatacccaa tgtctctggt tttggtaaat atgctcttaa aattggagaa
     6001 ctatactctg atactatttt gtattttatt ataaaaactt catctttctt aaaaaacttt
     6061 atataacttt ctgaatatgc atgcctttct tcaatatctc tcattaaatt ccatacttct
     6121 tttgattctt ttttattaaa ggtccctgaa attaaattcc catccaaagt ataaacccca
     6181 tactcatata tacttggaat tatatcttca gttacttttt cacttgaaat aatctgttct
     6241 ttatatttat taaattgttt ttctgcataa ttagctggta aaattacacc tgatgataat
     6301 aatacagaaa atgataacat taaaaggagt acaattgaaa ttgtcataat aaagaataaa
     6361 gataaatatt ttataaaaat agttcttaac ttttcaactt ttttgttgtt agatttttta
     6421 cctccaagca tttgtaccct gtcctccaat agtttaatta attcataatt aatacccaac
     6481 cagtttttta tttgattatt ttatatcttt ttattgctaa gttatatttt aacataataa
     6541 tataaataaa cagtcaattt ttactctatt cttctgattt tttaccttcc catttagaaa
     6601 accaaataca taaaattaca aatgctaaaa ttgttacaaa accacaaatt aaacctgttg
     6661 attgatatgc ttttattgat tcaaccttta tatattcaac cgttttagaa cttgaaaaag
     6721 acgaccaaat agttataaat ctaacacccc atgcacaagg aaaatactgc caaataccag
     6781 aacccaatcc agttaataat aatgctgaaa gtaacatttc aactatacct actccaattg
     6841 atgttccttt tccgaacctt aaacttaaga ataaatgtaa tatgtattca aataaattac
     6901 acccaattaa aattagaatt gaaatcatat aaaagcttga tgtaaagtaa gccttagata
     6961 taccaaattt aaaacctaat acagacacta atgttgctac aaaaccaaga catactaaga
     7021 ttatatattt acttataaga gtaagatatt ttggattaga tgttgttaac atatgtttat
     7081 aattccctgc ataatattct tgttcaacaa acattgaaca cacaacactt gacaataaag
     7141 gaaaagctat tgctataact tgtaaatatg ctactatctt tagtatatta tcagctcctg
     7201 aaaccttata ataccataaa aacaatccca tccccattaa agcaatgcaa atgtgcatta
     7261 gtattataaa atttctcttt aattttatta aatcagaaca aaatattctt attaactgtc
     7321 tcatattact tcacctctct attattgaac caaacccttg tagctaaagc aaataataca
     7381 aataaaacaa tagatattaa tactcctggc aaaataacag cattatttaa aaattcactt
     7441 ccctctggta caggaagacc atttggcaga atatgcaata caacagccat taatcttgaa
     7501 ggaatcgaat atggaaacat ataccacatt tctgtagttg agaaaacagc aactccaaca
     7561 atagtaaaga acatatttgt tagaactgta acaaacatac caaattttga agctaaaaac
     7621 atacaaagag gaatctgcca cataaatagt aaaataatta atatacttgc aatgatattt
     7681 tgacctattg aaatagtttc tttatagatt atcccaacaa aaactacacc tattgcagaa
     7741 ataattgatg ctataaaaca acaaataata cagtaaatca tttttccagt ccaaacgtct
     7801 ttcatttcaa ttggcaatgg taaaatacca cgatatttac ttttttcatc ttttaatact
     7861 attaaagaag atattaatgt taatgctcct ggaagaaatg tcgtatacca ccagttgaaa
     7921 gcagcatttt gccctccacc tagtccacac aaaagtatac aaaacactgg aactatccat
     7981 atcaacttca ttccaaaagt tcctttcatc ttttgaaatt ccactctaat aaatctttcc
     8041 atattactgc cctctctttc tattttcagc tacaactttc ataaaaagtt cttctaagtt
     8101 ttgttctttt gatacttctc cttgataacc cattactcca ttagaaatta ttcctatttc
     8161 atctacaact tgttcaactt cagacaagat atggcttgat aaaataactg taattccttg
     8221 ttttggaaaa gaacgaatca attctctaag ttcttcaata ccaatagggt caagaccatt
     8281 tgtaggctca tctaagatta aaagttttgg gtgatttaat aatgcaatag caataccaag
     8341 gcgttgcttc atgcctagag agaattgacc tgaacgcttt ttaaaggtat ctcttaaatc
     8401 tacagtctct aaaacttctt caatgcgaga gtcaggaaga ccaagtaaag tagtatggac
     8461 ttttaaattt tctcgtgctg ttaaattctc atatatagca ggagactcaa taagagaacc
     8521 aatatttgat aaatcctttc tagtccatct atgtccctca aacattattt ttcctgaagt
     8581 tggatttaaa atcccagtta tcatttttaa taaagtagat ttcccagctc catttggtcc
     8641 taataatcca tatatagaat tagatttaat tgctagtgaa atgttatcaa ctgccatctg
     8701 ctccttaaat tttttagaaa ctccttttac ctgcaaaata taattactca taattttcat
     8761 cctttctttt gtcaaactta tttacaagaa tcattctaaa tcataaatat aaggatttta
     8821 taagaattaa aaaacaaaaa aagatgtgtt aaaaacacat ctttttttat cttaattcta
     8881 ttaattttcc attgtagtat atctctactt cttcaatcca aatgctatta tcttcttctt
     8941 ggatgacatt tttaataata ttatttatag tatatattat attttgttca ttcaaataat
     9001 catctttcca agaaactcta ctttttttat ctggttctat caagtttatt ttaacaagat
     9061 tatctttttc cttaacttct acttccatgt ctaaaccatt aaaatactca tttgataatg
     9121 tatctaagat taatcgcagt ttatccttaa gtgaactttc ttcatcaatc tgtaagtact
     9181 ttactatttt ctcatccttt gaaattatct ctataggtat tttagtagtt tttttagaat
     9241 taatcatttt actagttgtt tcttcttttg catcttcatc cttctttaca actgctgatt
     9301 ctttagaatc aactagctca actgcactga tgctataatc catacatgcc gtactcaata
     9361 aagtagtagc aattattaaa aaacttactg ctattttatt cataagtatc cctctcctaa
     9421 ttctctttcg atacttatta taccatagta tagattatat ttttatactt ttaaatcaat
     9481 ttataaatta ttatagtaaa ttatttacaa ttctaagcta tttgaattta tttttatagt
     9541 atattctttc tctaaattcg acagattttt attcaaaagc ccttttattt ccctcgtatt
     9601 tctatacttg ttgctatgtt cttcattatg ttcataatca tatgaatcat tgtcatattc
     9661 ttcatcatta ttaaattctt caattgcttc ttttgacttt atatcacaat taaatttata
     9721 cttatttact ggtatatcaa gtcttacctt agaatttgga acttctatgt ccaggaactt
     9781 agcaattggc atatcagagt acaagtaaaa ttcactttca taatttgaat ttctacctat
     9841 tttctctttt atatttacat tttcaggtat atactcttct atattattga taaatacatc
     9901 tccattctct gaagatatat ccaatgactc tgattctatg tttacatttt taacgcctaa
     9961 aattgacttt gttgatagat gaagattgct attagatatt aagtttaagt tttctaatct
    10021 atttacttta ttttctgtta tagctctgct aaaacctcca gaagaagttt tatatgttat
    10081 attatttgaa tctacatcat cagatataaa taagtacgaa cttatacttg atgcatttat
    10141 atctacatta tttggtacat atattctaat ttctctataa tcacgtgaaa acatactatt
    10201 tatagatgta tttaataagt ggtcgaaact tctaatcttt ttaattttat tttcagattt
    10261 tctttcgcct tttacaacta aagtcttatc ttgtagggtt acactgtatt tgtaaaattc
    10321 attattccct cttgttgtca cacgcacatc ttcatttgaa ctttttctta tcataacatt
    10381 tatattgtca acagctatat ctaacttgtc aatatttact ttttcttgat aaagtttgtt
    10441 ttctttgttt aattcagctt ccttttctaa accaaagttt attaggcttg gcataactaa
    10501 aatccctgac catgttgtcc ctattatacc tattaccaat aaaactgcag caaatatagc
    10561 aagtttttta ttcatgttta tcttctcctt tcttattttc tatatcttca aggtccttat
    10621 atatcgcctc aaaatttata ctatcatcat tatattcatc agtttctttt atgctttctt
    10681 cttctctatt gtagtaatca ttattatatg aattttcatt aatgtcttta ttcaaatctt
    10741 catcatttaa agtagctttt tttctttttt ccatcttttc atttgccttt atataaatat
    10801 tttttctctt tatccaatgt atataattat ttagtaattg tttacctagt tttatggcaa
    10861 ataataaaat ttgccatgca agtatctcaa gccctacaaa tgctatggtt gcgaaaatac
    10921 aaaacatcat aacatctgga accattgcta taaatttaat cacaaatgga attaatataa
    10981 caaaagcaat taatgagcat actaatgcaa tagtagcaga tacaatagca catacaaacg
    11041 caaatgctgg aacaattaga aataaagata acactactaa taaccccttt ataacttgtc
    11101 tggataatcc tcctacacct tcttctatac ttggtgtaag tttatcattt atattttctt
    11161 ttgatttatt gtacttttcc ttagcttcat ctttcaagat agaataatcc ttttttacat
    11221 ttgatttaaa tctactaaat ttaccattta atatgtctgt attcattatt tttccatcta
    11281 tattttcttt tgatttacta tacttttctt ttgcttcatc ttttaagata gagtaacctt
    11341 ttttcatatc tgatttaaat ttactaaact tatcattcaa aacatctgta tttattattt
    11401 tttcctcttc aatatttttt tctttagttt gtaagataag ttcttttgct atagattttg
    11461 gtgagccaag tgatgctatt atctccatat cactctttcc ctctataaga ccatctacga
    11521 aatactcttc ataatctctc aatatatcat taacttcgct ctcagaaaaa tcttttttta
    11581 gataatcctt taaaatttca agaaactcag ttttattcaa gttcattacc tccctcatct
    11641 tcacttatta aaatctcaac agcctttaca aattctctcc actcttccat cagattggat
    11701 aaattctctt ttcctagtga agttattcta tagtattttc tagcaggacc ttcattagat
    11761 tctagtatat atgtttcgaa atacaaatct tttgtaagtc ttcttagaat aggatatatt
    11821 gttccttcat ttacatctat tactttagag atgttatgta ctatctcata tccatacata
    11881 tctttcttag atatgagagc aagtacacaa atttctaaaa caccctttct aaattgtgtg
    11941 ttcatatact ccatcctttc tttaaataca cagtattgtg ttttacaagg tactatgtta
    12001 aaacttctta tatcctcata ataacacagt actatgttct gcacaatagt tatttttaaa
    12061 tattcttgtt gattaaatcc ttctataaat gtataatact ctaatcagat atattttatg
    12121 aaagtggtga taaattggct aaaatgaaag atgataaaac aatattaatc ctaactgctc
    12181 aatttggtgc tggacatata agtgctgcaa aagcagtcaa agaatgtatt attgaaaaat
    12241 atagtaacta taatgttgtt atacaaaact ttataaatgc aagtattccc atgatgaata
    12301 agcctatggt aaaactttat gaaaacaata caaaatatac tcctggatta tataattact
    12361 attattactt taagaaatcg tttgattcaa gacatgattt atctcataaa ttatatacac
    12421 ctaaactttc tgagtatatt gctgatataa atccagattt gattatctct acatttccac
    12481 tagctgctgc ttgtgtaaat aactttaaga taaaaaatcc tgatataaat atacctacac
    12541 ttacagttat aacagatgtt gtagatagta tggaatgggt ttttgaaaat acagatttgt
    12601 attttgttcc ttctcctgaa ataaaaaata gattttttca gaaaggaata aatccagact
    12661 ccataaaggt tactggagtt cctgttgata agagatttca aattgaaagt aaagaactat
    12721 gttgtgataa atatagacta ttacttctag gtggaggaag aggtctattt gatatagatg
    12781 aggattttat gcattggata gatgaattta ttgaagaaca tagtaattct atagaaatta
    12841 caatagttac tggtaaaaac aaaaaattat atgacaatct aactcataaa aaacctctaa
    12901 aaaatattaa agtacttgga tttgtaaacg acatgtataa tctaataaga gaatgtgacc
    12961 taatgctaac taagcctgga ggagcaacta tatttgaagc tattcaatca caaactccag
    13021 tactcgtaaa aatgccaaaa gttggacaag aaattgaaaa cgctaaattt ataatagata
    13081 agggtcttgg catgatttat agtgatgacc tagaccttaa aaacatattt tataaattag
    13141 tatcaaatga atttgattct ataataaact ttatgaaaaa aaatttaaaa gaatttaaaa
    13201 cagtcattca tccagagaaa attgctgatt atatatcaga attaatagat aatcattaca
    13261 gttaaattgt actattaaaa aatttcaaac ataaaattaa aggcaatcaa gtatgctttt
    13321 acacttgaga atttactcat agtgtttaat aatacttgat tgcctttatc atatcaaaat
    13381 caaattgtta tattagtata tatttttctt gtactgcata tacagttgtt gtatttcttc
    13441 catacttctc tttaattcta tttgtaaatc tgaagcagtc tggtaatcct tattttctat
    13501 agcttctctc actctagtga atagtgattc tctagttaaa gtatccttca taatattgaa
    13561 ctttaaatcc gatattgagt tccaaactac ctcatcaagc gcatccatat tttcttttgt
    13621 gtgtatcttg acaatacttc ttattttttg aatcccagcc tctattattc tagtttttaa
    13681 tttcttttgc cacttatcta tagctccaat cttaaatgat tctattatac tatcagtaaa
    13741 tacatcacct tgttttaaag attttaattt tgaagcatat atttctaagt ttttcatatt
    13801 ttcataaact gtagccggtg gtattccaaa tcttgcattt ctttcttcca aactataatg
    13861 ttcaaataca tcattttcat ctctatatgc tctatctttt tctaaataga aactttcttc
    13921 acctacattt ttagataact ctttttcaag ttcttttgta ctaagaccac tcttagctgc
    13981 tgcttttata ccatccaaca ttgtttgata acatcctgct attactaaat acgtgttaga
    14041 tagagggctt ggtgacctta actcaaatct tacagtcttt ggatttttca tatctcttat
    14101 taatccaaca agcactgacc tatttcttga tggaatctca taactatgac ctaaagatgt
    14161 aactatgcat acaggagctt caaatccagg aactaatcta ttaaatccat cattagaagc
    14221 tgttacaatt gggtttaata cttcataatt gtaaagaagt cccataagag ccccatatcc
    14281 tagttcactt agataatctt cttttaaatc ttttggcgca aatagatttt ttatacttcc
    14341 atcctttaac ttcgcactta ctcctacatg tgtatgtcca ccacttcctg ctactccatg
    14401 aattggtttt gctttaaatg taacttctag tccatgtgat gtaaatacat cctctataac
    14461 atctcttact aataattcat tgtctgctgc ttgtaacggt gtagaaaact tccaagaaac
    14521 ctctaattgc tccatagcat ggtttgttct tccatctata ctaatagaac tttgtattcc
    14581 tccgacctct ttgtgagcca tttcagggtt tacacctagc ttttgaagta taattaaact
    14641 tttctctaaa caagttctta taataccatg agttcttttc cagtattgct cttttaggct
    14701 ttgtgataca tataatttct ctaagtcagc tttatcctca ggagtattaa cccagaactc
    14761 aagttctgta gctgctgtta acattatttc ctctatatca tctactgaat ctattcctat
    14821 gttatttatt acatgtggat attctctaaa tatttcatac attgactttt taaagtactt
    14881 atctgctttt tgaagtactc ctctagaaca tactttttta ttatcatgta ttaaaaatgc
    14941 aggtatcttt aatgttccaa caggtagacc tacttcttcg tctatatatt ccatattgta
    15001 atctacatac caatgacaac ttttatctgg cattaaatct actttcgcat tatttaatgt
    15061 agctatatta taaagctcaa cacttgaacc atcagtctgt atagctgatt ccaaaaattt
    15121 atctatatcc tccaaaaata attctacagg aatcttttca tctgttgcat ttcctcctaa
    15181 atcaaccccc attaaggaaa caaatcttat atttttattg ctttttagga tttctcttaa
    15241 ttcttcatta gtgtgtttat ctttctctat tacgtataat agactatcca tttttccact
    15301 ctcccataat ttttatgtat attttatttt gttaaataca agataaattt cattttcctt
    15361 acatttatga atttttcttg caaattattc gtgttttatt cgtgttttta tttatattat
    15421 tatatatcaa gtataaaaag ttcgcaatat tttttattgg aaaaaatatt atacatacaa
    15481 tagtttaaca tttttacaaa tatggggtat actaaaataa aagtattttt atttgaaaat
    15541 ttaattttat ttataatata atctatagta aaagaaataa taaaaaaatt taggagataa
    15601 actttatgga atacataatt ttcgatttgg aatttaacca aggatttgat aaaaaactca
    15661 ataaaactgt atccaatgaa aagtgtcctt ttgaaattat acaaatagga gcaattaaat
    15721 tggattctaa attcaatata atagatacgt ttaatagcta tgtaaaacct acaatttata
    15781 aagacataca tccctttatt agtcgtatga ctaatattaa aagttctgat tttaatgatt
    15841 cgcctacttt ccctgaggtc tacaacaatt ttataaaatt tatatcatca caagacccta
    15901 tattatgtgt ttggggagct ggagatttga aggaattata caggaatatc aactatcaca
    15961 agctaccatc aaattcctta cctaaatctt atattaatat acaacaacat gcatctaaat
    16021 atttcaataa tccagcagga aaaagcattg gcctacaaaa tgctatttct atattggaac
    16081 ttgatgaaaa aatgtcttat cacaatgctt taaatgatgc atattatact gctaaagttt
    16141 ttattaagat atacaatcca agtatagtcc cagatatata tctttatacg agtataaaac
    16201 ctaaaactat aagatattct aacaaaaaaa gagttgatta tgataagtta tttgatgaat
    16261 ttaggaaaat tttaaatcgt gaattaacta aagacgagaa aaaaatcatt aacttagctt
    16321 ataatatgtg gaaaacaaat caatttacat tagaaaatgt taagcaaaga aaaaacaaat
    16381 agagtttatt tagataaaaa ataggcatgt agattaaaaa actagttaac tttaaattta
    16441 tatctacagc ccaattttta tcataattaa tttttatttt atctctaatt caactggaca
    16501 atggtctgaa cctaatattt cagtgtgtat atccgcactt actaatctat cttctaattt
    16561 tttggatgca caaaaataat ctattctcca ccctgcatta ttttttcttg cattaaatct
    16621 atacgaccac caactataaa caccttcttt atcaggatta aaatatctat atgtgtctat
    16681 aaacccactg tctaataatt tagaaaactt ctctctttct tctttagtaa aacctgcatt
    16741 tttcatatta tttttaggat tttttaaatc tatttcttta tgtgcaacgt tcatatctcc
    16801 acacacaatt acaggcttat gattatctag tttggttaaa taatctataa agtcgtcttc
    16861 ccatctagtt ctatattcta gtctctttaa ttcagattga gaatttggcg tatatactgt
    16921 aacaaagtaa aaatcttcaa actctagagt tatcactcta ccctctttgt catgttcttc
    16981 tatatttatt ccatacatta cttttaaagg ctcttttttt gtaaatatag cagtacctga
    17041 ataacccttt ctctcagcat aattccaata ttgaaaatat cctggtaaat ctaactctat
    17101 ttgcccttct tgcaatttag tttcttgtaa acaaaatata tctgcatcta cttctttgaa
    17161 aaagtctaaa aatcctttcc caacacaagc tcttattcca ttaacattcc atgatataaa
    17221 tttcatactt atactcctca tctaaacatt gacacactcc ccatagctaa agatgggggc
    17281 ttttcaacaa ttttattgta aattcttaat ttacttttat tttaacaatg ctatactata
    17341 atttcatcta aaattactat ttacttaata cctaaaaata tatgttttaa tcaattttta
    17401 acttttttgt taaatcttca gggctcattg aagaaacata catatttgtt ccaagttcaa
    17461 atcctccaaa attatatttt cttgggatta tgtgcatatg aacattaaaa tacgattttg
    17521 tttcaatctt tgttggatga gtgtgaatac ataaattaaa tggcatatat ccattgatat
    17581 tgtataactt cttaaacaac ttttcaaata ttgtagaaag ctcctgtata ttattatcat
    17641 ttaactcacc aaactctatt ttatctttaa atattattct tacttctcca ctatacttgg
    17701 ttgcatatgg tataagtacc aaaaaagctt ctccattata aacaactctt ctatctaaac
    17761 ttatttcatt ttctattaaa tcatcataca aagagctttt attgctgtca taatactcct
    17821 ttgaaaccaa cagttcattc gtaatttcag gtggaataaa agacatagac aatatttgag
    17881 catgtggatg catcaaagat gctcctgcct ttctcaagaa gtttttgaag atactaacat
    17941 actctacttt atcatctttc gacaaatctc taaatctatt tctatacata aaaaaaacat
    18001 cttcaaattc tttttgactc atgttataaa aactacgatt atgtttatcg ctctctatca
    18061 ttacttcatg aacaccatat acatctcttg ccatatcatc aattatagga aatttattgt
    18121 agacagattt tacaagccat ccttcttcac tatctatttc aaataatgca tcagttgcat
    18181 gagcttcatt tcctttgcaa aatgggcatt cttcatcata ttcattagtt tcttcatctt
    18241 catttgggat atctgctgta tctaaaggtc tttttaacct atctgtagca aatataacaa
    18301 catcattggt aattggatct attcttaatt ctttcataat taacacctct tagttaatag
    18361 cctttaatat aattatatga tatttatcgt atctgttaaa taaatttata atatttttta
    18421 taattttgca tatttattat tatatatttt gtctatagct gaaagattat aactaaaaaa
    18481 tcactatcag tacatcagaa tattatctta ctataaatta atttatgaat acataattta
    18541 gtggcataaa catgggtatt ttactataat ttgttaaaag tttattttaa agctatatta
    18601 attttttttg atgattttta cccataagta tgttattatt atcttgtaaa gatgtcttgt
    18661 attcaaacat acaatttaca taatacaata tacgtattta aattaaatag ggggtattaa
    18721 aatgaattat tcaaacagag ttagtgcaat gcaagcctct ccaatcagaa aacttgttcc
    18781 atttgcacaa gcagcaaaag ataaaggaat caaagtatat cacttaaaca taggacaacc
    18841 agacataaaa actcctaaag gattttttga tgctgttaaa aactttgata gcgaagtctt
    18901 agaatatgct acatcagaag gtattccaga attattagaa gcacttcaaa attactataa
    18961 aacttacgat atgaattttg aaaaagatga attattagta actaacggtg gtagtgaagc
    19021 tttacttttc actatgatgg cagtttgtga cccaggagat aatctattag ttccagaacc
    19081 attctataca aactacaatg gatttggtca atctgtaaac gtagaagtta atgctgttac
    19141 aacaaaggca gaaaacggtt tccaccttcc atctaaagaa gaaatattgt ctaaagtaga
    19201 tgataaaact aaggctataa ttctttctaa cccaggaaat ccaactggtg caatttatac
    19261 aaaagaagaa cttaatatcc tagctgaaat agctaaagaa aaagatttat ggataatagc
    19321 agacgaagtt tatagagaat ttgtttatga tgggcttgag tatacaagct gtggtaactt
    19381 agagggtgta caggatagag ttataataat agacagtgtt tctaagagat acagtgcttg
    19441 tggtgctaga attggttcta tcgcttgcaa aaataaaggt cttatcgctc aaatacttaa
    19501 attatgtcaa ggaagacttt gtgttcctac attagagcaa ataggagctg ttgagttata
    19561 caaaactcct gtttcttact tcaaagaagt taatgaagag tacaaaaaaa gaagagatgt
    19621 attatacaat gaattaatga aggttgaagg tgttatatgt aaaaaaccaa ctggtgcttt
    19681 ctatatagtt gctaaattac cagttgaaaa tgcagaagat ttcacaattt ggatgttaaa
    19741 agaatttaat aaagataatg aaacagttat ggtttgccct gctgaaggat tctatgctac
    19801 tccaggttta ggacgtgatg aaataagatt agcttacatc ttaaatgaaa aagaccttca
    19861 tagagctgct acattattaa aagaaggttt agagcaatat gtagctttac aaaaataatt
    19921 ttttaaactt aatttatata aatttaatga tataaaatgt ccccttttca gatattattt
    19981 gaaaagggga catttcttaa ttaatatatt aattaagact tagacctgac tatttttcat
    20041 tttctttata tagtgttggc cagtaatctt tattcacttt aattaaagca tctaaaactt
    20101 gtcttgcctt agttagagaa attataaaaa agtacatcaa gaaaatttaa gatgtacttt
    20161 ttagattgta tttagactta taattatata gttaaatgaa taacaaagaa ttaatcttag
    20221 taccattaaa tacaaaaatt tttatatttt ttctatctaa attcttcttt gtttaatata
    20281 tattgaagag tatttattgc ttctaaatat ttttttctgt cttcttcagt taatttagaa
    20341 agtttttctc ctaaaccatc acttaattta tccataaaat cagttgctat cttatctccc
    20401 tcttcagtaa tgtcaattag atattttctt ctatcaacta aatctctttt ccttgttaca
    20461 tacttcttat cagttaaatc atcaaccata ctagttaaac tgcctttttc tatatttaac
    20521 ttagagcata aatcagtcat acttattact ccataatttt ttatgaaaac taatgctctt
    20581 aattgtgttt tatttacatt ataatctggt gcaaattttt ttaggtcttc taagtacagg
    20641 ctagaataaa tcttaggaaa tgtttttact aaaaaactta tggtatcttt tactatatag
    20701 ttcatataat cacctcggtt gttttaaaat ttattaaatt atctaaataa ctttatttca
    20761 gtttacatta aaaatattgc aagtacaata gtttttgctg aatgtttaaa tttaattagt
    20821 aatcttatca tttttttatt tttatttata ttattcctat taaatattat ttatatagct
    20881 attttattaa attcatggta tagtaagtaa agaagaactc ttcacagcgg tggagagaag
    20941 acgctagccc attaaaatgg ctatcttatt tataaataag atagccgttt ttaattatat
    21001 tttatctgta caattctata ctcttaggtc tacatctact tttcctaagt cttccttata
    21061 atcttttata gcaggttcta caacttcatt tacaaattct acaacttgtt ctggagctct
    21121 acctataaag ttttttgggt ccattataga tagtatttct tccttgctca ttttaaatga
    21181 atcatcattc attataagtt caattaagtt attatccttg ccttcatgct ttactctata
    21241 ggcagctttc atagaatatt ctcttattat ttcatgcaat tcctgtctat ctccacctct
    21301 ttttacagct tccataagta ttgtttctgt agccataaaa ggaagttctt catttacacg
    21361 tctatttatc atattttcat atactactaa accatcagtt acatttatcc ctatttcaag
    21421 tatagcatct gctgccataa atgcttgagg tattgccaat ctcttattgg ctgagtcatc
    21481 taatgttctc tctaaccatt gagtggcttg taccattgca gggcttatag attcagagat
    21541 tatatactta gataaagatg ctattctctc acttctcatt ggattcctct tataagccat
    21601 tgctgatgaa cctatttgat ttttttcaaa tggctcttct aattctttta aactttgtaa
    21661 aagtcttata tcattagtca tcttatgcat actttgagct atccctgata gtatgcttat
    21721 tacttgataa tctaactttc tagtatatgt ctgtccactt actgcatagg aagaatggta
    21781 tcccatcttt ttacaaattt tcttatctaa ctcttttact ttttcgtggt ctccttcaaa
    21841 taagcttaaa aaacttgctt gagtaccagt agtaccttta actcctctta acttcatgtt
    21901 gtctattcta tagtttaaat cttccaaatc aagaattaaa tcttgagccc ataaacaagc
    21961 tctttttcct actgttgtaa gttgagcagc ttggaagtgt gtaaatccaa gagctggcat
    22021 atctttgtac ttaagtgcaa attcttttaa attgtttatt aaatttacaa gctttactct
    22081 tagaagtttt aaagcttcat tcatttgtat aacatctgta ttatctccaa catatgcaga
    22141 tgttgctcct aaatgtatta ttcctttggc tttttcacat tgtaaaccat aagcttttac
    22201 gtgagacatt acatcatgtc ttacttcttt ttctattttt ctagcttcat caaaatttat
    22261 attatctata ttagctctta gctcatctaa ttgttcttca gttatattga ttcctaactc
    22321 atgttcacct tctgcaagtg caacccataa ccttctccat gtagagaatt taaattgtgg
    22381 agaaaatata taactcatgt ccttgctaca atatctgtct gtaagtgggt ttgagtaagt
    22441 agtatattta ttatctatca ttatattagc accttcctta aatcttttgt cgtgtgatta
    22501 ctgtgtacac attagtacat aaataattat atataatttt acgaactata acaagacttt
    22561 tttatttttt gttcacattt atatatagta ttttttattt attactattt tttatttata
    22621 tccaaattat ctttttcaat taagtaatcc actatttcac tatcaacaaa atttaataca
    22681 tacttatatc taattgcata gtatttatca ctccatccaa gctgattttt tacatcactt
    22741 tcatctgccc caagccttaa acttattgct gcaaatgaat gtctaaaatc tcttgctgaa
    22801 tattgagaaa gtccagctaa atctaatgct tttttgacta taagtctgac atttctatct
    22861 gttatagaat tactcttttg agttgtaaaa ataaaatcat cactaggtat tattacctct
    22921 ggaagcccag aataattcct gtaatcttct aaaagtttaa aaaagtatgg atgtaattta
    22981 actactcttt cttttcttcc cttacctaac cttacatatg agttatcgtt ttcatctacc
    23041 ataaggtctt tccactttaa gctaactaat tcatttaata aacacccagt agtaactaga
    23101 cagactatta ttactctatc tcttatattt aattttggta ggatacctat aagtttattt
    23161 atttcttgta tacttagaac atcatctttt gtttttattc ttgttactgt gggtttttct
    23221 acatatctaa atggatttat atctatgtat ccttcttttt ttaagaagtt ataaaaacta
    23281 tgtaaataac tatatatctt ttcacaagtt gatttcgcat atttagtctg tatatagtct
    23341 acaaaatttt tacaatcttc tttcgtagca taaatcaatt ctttcccttt taaaaattct
    23401 ttaaacagta taacctttga taaataatcg tgtttcgtgt ttggatttaa ttttgtagaa
    23461 aaaacattaa aagtttcata ctcataatca tctagtattt ttgtactata cataataact
    23521 cctttccttt tcataaaata aaatagctat acttttacta taccatatat ttccccctca
    23581 aatatacccc ttattcaata gataactttt aagtttattt tgttatatta tgaaggtttt
    23641 atgaaaatat caatcttcca ttgtgcacta cacagtacta tggtattata agttagtaaa
    23701 aggtggcatt tttttattaa tctatgaata aaatatgcta ctataaattt gtattggagg
    23761 tattttgatt tggaaacatt tagtttgctt gaaattttta aggcagtcat tttgggcata
    23821 gttcagggaa taacagaatg gctaccagta agtagtacag ggcatatgat attagttgat
    23881 gagtttataa aattgaattt ttcaaataca tttataagta catttttagt tgtaattcaa
    23941 tttggttcaa tattagctgt acttgttata ttttttagaa aattaaatcc ttttgatagc
    24001 gcaaagaata ttaagcagaa aaaagaaaca gtccgtttat ggcttaaggt tataattgca
    24061 gttataccat ctggagttat tggaatactg tttgaagatg atatagatag attattcttt
    24121 aattcaacag tagttgctat tgctcttatt gtatatggta taataatgat agggcttgaa
    24181 aagagaaata aaaaacctaa gtataaagat ttttctcaag ttacatataa attagctctt
    24241 tgtataggat tattccagtg tctagctctt ataccaggta catctaggtc tggttctact
    24301 ataataggtg cagtattact tggtacttct agatatgtag cagcagagtt ttcattcttc
    24361 ttagccatcc ctacaatgct tggagctagt gcattaaaac ttctaaaagc tggatttgga
    24421 tttactggat ttgaatggct tattttaggt gttggttctg tagtagcatt tgttgtttca
    24481 attgtagtta ttaaattttt tatggactat atcaaaaaac atgacttcaa agtatttggt
    24541 tattatagaa tagtcttggg tatagtggtt cttgcttatt tctttctatt ataatgtatt
    24601 ctaatttaat aacaaatgat aaggagaaaa gttatgagtg ttaaaaaaga aatatttgat
    24661 tggattaagt caatagctat ggctattgta cttgcatttg taattctaca atttataaga
    24721 ccttctattg taagtggaga atcaatgtat cctactttag atgataaaga ttatctgatt
    24781 ttaaatagga tatcatacaa ggttggtaaa cctgaaaaag gcgatattgt agtttttaaa
    24841 accaatttag ttgatggaga aacaggaaag aaaaaagact taataaaaag agttatagct
    24901 actgaaggtg acagaataaa aatatcaaat tctaaagtgt atgtaaatgg aaaattatta
    24961 aatgaaccat atatacacaa taactatact tctggagata tagatactgt tgttccaaaa
    25021 ggtaaactat ttgcaatggg agataataga gaaaatagta atgatagtag attccctgat
    25081 gtaggtatgg ttgatgaaga tgaagttctt ggtaaggtta tggtgagact attacctctt
    25141 gataatattg ggaaagtaga ctaaaaaata agagactgaa ttggtaaagt ttattttaca
    25201 aatctcagtc tctttttatt ttcttaaatt acaaatattt ttttactaaa tatcttttga
    25261 ttctgtaata agtcttatta tatcacttgc ttcttcatta aaagttgtta ctatacttag
    25321 gtagtctgtt gctctactca tagctaaata aaccttattt aaatctccta caaagtcatt
    25381 tacttgataa tcttgttcaa tatcatttat agtttgattg tataacatct ctaattcaca
    25441 tactattacc gctttatact ctaaattctt tattgcatat atatttgcta ttgtagttcc
    25501 taccttctta cttatatttg tcaaattatc ttctgcacat atataaggaa tttttgcctc
    25561 ttccaatgct ttttttaaca tatattgaaa gtaaatggtt tttccacttt ttaatttctt
    25621 cttattgtaa ggataaacga tagcaacttc agaataatca agaccttttt tacttattaa
    25681 atactcaatt tcccagatta cagaacttat ttggtcatca aggtcactta cttttattat
    25741 atcaacagac ttatttccac ctctaagtgc tttagtataa aagaaagtac taaattctgt
    25801 atttggtctt aattcgttga tatatgagtt agaattattt gaaaatttat ttgtaaaatc
    25861 tacaatctct ttagcttgtc tataattttt acttaagata atcttatcat caaattctat
    25921 accattatat aattttttaa atatattcaa tctattagag atattcaagg agttacaaga
    25981 acacacatta aatatgaatt ttgttttata taaaaactcc cttataaaat ctatttcata
    26041 ctctaaaaag ctttctgctt catcaataaa aatagccttg aacatatttt tattttttat
    26101 aatgttctgt gcttgtttta caagattatt aaaggtcttt tcgtaatcat tttttaacat
    26161 gttataatct acaattaaat tgaatttttt agctagttta aatataaaag aactaaatgt
    26221 gtgaacttct aaattgttat tatctttgta taataactct atcctctctc gaagctcatt
    26281 gcacagttgt tttgtatgtg taaatatgac taatttatga tgtggatata cttttgctaa
    26341 ttttatagcc ctagaaagca taattgctgt tttgcctata ccagaaccac cttcaataag
    26401 tgtattccca tatttaatag aaacaacatc tttaatttga acgtcttcca tcatagttgc
    26461 tgtatactta tagtcatcat tgtagaatga aattttttta aatttttcat ttagatgaag
    26521 tttatcattt attatatagt attctgaaca tatgtctagt aaaaataaat ttaagtttat
    26581 ttcatcattc tcatcattaa gatattcctc cagcgaaccc ttattcatta tatcttgaag
    26641 cttattttta tctattatat tattattaac aaattcttca aattcatatg tttcctcaac
    26701 ttcaacgtat ggcataatgt aaacgtagtt ataacttata tttctattct tttgtcccat
    26761 ttttagtttc aaaagttcat attcctcatt cataacttca agtaactctt cttctaaaat
    26821 aaaaaatata tcttctgttg tatccataaa ttttataaat aaaattcttc catctttaac
    26881 atacaacaaa tcggtattta ttcctttaaa aggagtaact tttggcacta ctttaacgtc
    26941 ctcattgata ttttcgtaaa atattttctc gaactccttc aattttaggg cgtctaactg
    27001 agaatttata tttatgtatt ggctcactta cattacctcc ttctgtt
//
LOCUS       DAYATN010000042        26098 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.042, whole genome shotgun sequence.
ACCESSION   DAYATN010000042 DAYATN010000000
VERSION     DAYATN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.042"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            742..2412
                     /gene="pdcB"
                     /locus_tag="ACGI8A_001702"
     CDS             742..2412
                     /gene="pdcB"
                     /locus_tag="ACGI8A_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase PdcB"
                     /protein_id="HFH0179701.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2534..3268)
                     /gene="pflA"
                     /locus_tag="ACGI8A_001703"
     CDS             complement(2534..3268)
                     /gene="pflA"
                     /locus_tag="ACGI8A_001703"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFH0179702.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNIDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3428..5659)
                     /gene="pflB"
                     /locus_tag="ACGI8A_001704"
     CDS             complement(3428..5659)
                     /gene="pflB"
                     /locus_tag="ACGI8A_001704"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFH0179703.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPGALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            6018..7013
                     /locus_tag="ACGI8A_001705"
     CDS             6018..7013
                     /locus_tag="ACGI8A_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFH0179704.1"
                     /translation="MFITVVLFLVGFLLITKGADIFINCTVEIGKKTNISEIILGATI
                     VSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICNTGLALGLVVFISPFNVDKKMFF
                     SKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIFYMVNNYRSVVGKSDTKNRNIKN
                     NTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMMIIGSQILIESGVIIASFLNIPQ
                     GIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNILGANILNIVSVIAVSAIPNNIP
                     ILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGILMLFTYFLYISILYFMYII"
     gene            7104..8717
                     /locus_tag="ACGI8A_001706"
     CDS             7104..8717
                     /locus_tag="ACGI8A_001706"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFH0179705.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            8992..10977
                     /locus_tag="ACGI8A_001707"
     CDS             8992..10977
                     /locus_tag="ACGI8A_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0179706.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            10982..11416
                     /locus_tag="ACGI8A_001708"
     CDS             10982..11416
                     /locus_tag="ACGI8A_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFH0179707.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKNLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11445..12005
                     /locus_tag="ACGI8A_001709"
     CDS             11445..12005
                     /locus_tag="ACGI8A_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFH0179708.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12069..12569
                     /locus_tag="ACGI8A_001710"
                     /pseudo
     CDS             12069..12569
                     /locus_tag="ACGI8A_001710"
                     /inference="COORDINATES: protein motif:HMM:NF015569.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
     gene            12609..13028
                     /locus_tag="ACGI8A_001711"
     CDS             12609..13028
                     /locus_tag="ACGI8A_001711"
                     /inference="COORDINATES: protein motif:HMM:NF019286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HFH0179709.1"
                     /translation="MAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFP
                     LLSPFLGPGAVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAE
                     AEPDTVQVGVPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13069..13434
                     /locus_tag="ACGI8A_001712"
     CDS             13069..13434
                     /locus_tag="ACGI8A_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFH0179710.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13535..14308
                     /gene="srlD"
                     /locus_tag="ACGI8A_001713"
     CDS             13535..14308
                     /gene="srlD"
                     /locus_tag="ACGI8A_001713"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFH0179711.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14657..16801
                     /locus_tag="ACGI8A_001714"
     CDS             14657..16801
                     /locus_tag="ACGI8A_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFH0179712.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            16889..17224
                     /gene="spoIIAA"
                     /locus_tag="ACGI8A_001715"
     CDS             16889..17224
                     /gene="spoIIAA"
                     /locus_tag="ACGI8A_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HFH0179713.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17238..17666
                     /gene="spoIIAB"
                     /locus_tag="ACGI8A_001716"
     CDS             17238..17666
                     /gene="spoIIAB"
                     /locus_tag="ACGI8A_001716"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HFH0179714.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17689..18453
                     /gene="sigF"
                     /locus_tag="ACGI8A_001717"
     CDS             17689..18453
                     /gene="sigF"
                     /locus_tag="ACGI8A_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HFH0179715.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            18733..19170
                     /gene="spoVAC"
                     /locus_tag="ACGI8A_001718"
     CDS             18733..19170
                     /gene="spoVAC"
                     /locus_tag="ACGI8A_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HFH0179716.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19337..20350
                     /gene="spoVAD"
                     /locus_tag="ACGI8A_001719"
     CDS             19337..20350
                     /gene="spoVAD"
                     /locus_tag="ACGI8A_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HFH0179717.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20364..20726
                     /gene="spoVAE"
                     /locus_tag="ACGI8A_001720"
     CDS             20364..20726
                     /gene="spoVAE"
                     /locus_tag="ACGI8A_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HFH0179718.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            20909..21925
                     /locus_tag="ACGI8A_001721"
     CDS             20909..21925
                     /locus_tag="ACGI8A_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0179719.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            22300..23142
                     /locus_tag="ACGI8A_001722"
     CDS             22300..23142
                     /locus_tag="ACGI8A_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HFH0179720.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            23157..23621
                     /locus_tag="ACGI8A_001723"
     CDS             23157..23621
                     /locus_tag="ACGI8A_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179721.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            23635..24780
                     /locus_tag="ACGI8A_001724"
     CDS             23635..24780
                     /locus_tag="ACGI8A_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0179722.1"
                     /translation="MNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPELAFEEYKSQ
                     KALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPGMGHACGHNI
                     IGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFNNVDAALILH
                     PSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSVNSMRLHLKD
                     YARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKGAAISTGTNV
                     EIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGNLTHEIPAIQ
                     AYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            24824..25759
                     /locus_tag="ACGI8A_001725"
     CDS             24824..25759
                     /locus_tag="ACGI8A_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HFH0179723.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
BASE COUNT        10090 a         2945 c         4596 g         8467 t
ORIGIN      
        1 tacaaaatca atgaaaataa gtatatccat tttaaagtac ataaatttaa aaatccagaa
       61 aaatatactt aaattatgta agtaaaagag gggttgaatc agttgaaaat gtaatattag
      121 taaatagtac atcaatccta tataccctgt aagcaactct attttcatgt actaaaaatg
      181 tacctatttt attaactgaa aagaataaat taaatgtaag tacataagaa gatagaaaaa
      241 attaaaaaca aaaaatattt atataaataa atataaaaaa tgatataatc cctattaaaa
      301 aatttataga tgatgaagaa atatcaaaat cttatattat aggaagagac acttctttgc
      361 caaaatctat agaaaagcaa taccaaatcc aaagagaata ggcgaagaaa aaaataaaat
      421 aaattcaaga gtaatagaaa agttttatag aataaagaac tgataactct ttatataacg
      481 aaaaatagaa ttttaagaga aaatgattta ataaatgatt taacatggtg ttttagcagg
      541 taaaaataaa tcaccagtaa tgattataga aaaattactt tatgaatctc aaaaaactta
      601 gttaagagta agagatttga taagataact aaagttgggg aaatgtgttt gatgagacaa
      661 aaaattagta aaatagtata atctaaataa ggatattttg ttgattaaac tacactaata
      721 gtaatattgt agggggaaaa gatgagagaa gaatcgaact ctttaagttt agaccatata
      781 gaaattttct ttgaactttt atctcaaagt acagaagatt atatattttt ttgggatata
      841 aataggaata aatttaaaat ttcttcagca atttttgatg aatttaactt aagtaaagag
      901 atagaatctg atttggtcaa ttgttggagt aaaattgttt atccggatga tgttcaaata
      961 tggaaagatg atatacaaga attactacat ggaaaaaaag gtgagcacaa tttagaatat
     1021 aggttgatta ataaatataa agaaatagta tggatttctt gtagaggtaa agtttatgtg
     1081 tcggatgacc caaaaactat atttctagtc gggagaataa agaatattgg tgaaaaaaat
     1141 aaatttgatt ctatcacagg aacgtggaat agagaacaat ttgaacatag aatgaattat
     1201 cttataaaag agaaaattta taaaaatggt gctatgttta ttatggatat tgataatttt
     1261 aaaaatataa atgaaaaata tggacattct tatggtgaca aagtattgcg tgcaattgct
     1321 actgaagtat tagaatatct tccaaaagat gtaagattgt ataggttaga tggagatgaa
     1381 tttgcatttt tttaccctat gtgtactaaa gaaactatag agaaaatata tgaaaagata
     1441 cagatgtata caaatactca gcatgaaatt gaatcaaata agtactattg cacagtgact
     1501 gcaggcgttg ctatgtatcc agaagatgga gataattatt tagatttatt taaacatgct
     1561 gatattgcat tggatatagc aaaaattagt ggtaaaaata gaattaagtt tttttcacaa
     1621 gagctctatg aaaataaact aaaggtaatt tcaatgcaac aaaaactaag agagtgcgtt
     1681 gaaaataatt ttaatgattt tgagttgttt tttcaacccc aggtaaatgc tgttacaaaa
     1741 gaggtaatag gggcagaggt acttttaaga tggcattcga gtacttatgg agaagtatct
     1801 cctgttgaat ttataccaat actagaacag agtaatttaa ttataccagt tgggaagtgg
     1861 attataaaag aagctgtaaa acagtgtaaa gaatggcata aaataaatcc agactttaaa
     1921 atatctgtaa atgtatcgta tattcagtta aaggaagatt tttttagaga ttttatagta
     1981 gaatgtttag tagagtatca attaagacct gaatttttaa ttttggaatt aacagagaat
     2041 tgttggatac cagatataaa tttactaaat gataaattta ttagtttaaa gggtatagga
     2101 gtatatattg caatagatga ctttggtaca ggatattcat ctttaaatta ccttaaagaa
     2161 ctgtctgtta atataataaa aattgaaaga agttttgtta agaatattac atataatagt
     2221 tatgaatata cttttcttga atatattatt aaattagctc atattataaa tttaaaagtt
     2281 tgtgttgagg gaatagagtc ttatgaagag tatgatatag taaaaagctt aggtgtagat
     2341 attattcagg gttttttatt tggaagacct gtaagtgcct ctgaatttta taaatttcag
     2401 ctatcaaagt gatgtatata aaatgagata tatagtatat aaaaataggg caaagtggaa
     2461 gttttgaagt ttattaaaaa tttacacttt accctatttt gttatgtctt ctataaagga
     2521 ttaatatact acactaacta ttatatactt tcactccaaa tttagagatt atttcactag
     2581 ctttttggac atcttcctta gtggcatcag gaacatcttt tagttcataa tcgataccta
     2641 aactttccca tttgtgtatt cccatagaat ggtatggtag caattcaaat ctttctacat
     2701 ttttcaatgt agctacaaat tgacctagtt tttctaaatt atctatatca tcagtaatgc
     2761 caggaactaa aacatgtctt atccatacag gaatatttct tgattctaag tgtctagcaa
     2821 gttttaatgc cttatccata ccaacaccag tcaaatcaat agatttttcc tcaaccatat
     2881 gttttaaatc aagtaaaacc aaatcaatat tatctaaaac tgggtctata gtttctatat
     2941 ctacaaatcc agatgtatcc aagcaagtgt gtatatcatt ttcttttgct tttttaaata
     3001 attcacttaa aaattctggt tgaagagtgg attcaccacc agaggcagtt atacctccac
     3061 cagaaaactt cataaaagaa gtatacttaa gagcatccgt tattatttca tcaactgtgt
     3121 attcttttcc tgatttaata tcccatgtat ccctgttatg acagtatttg catctaagag
     3181 gacacccctg aaaaaataat atatatctta ttcctggtcc atctacagta ccaaacgttt
     3241 ctatagaatg tatctttccc ttaatcatag agttcacctc acaatatatt ttaataacct
     3301 tatcaatagt tataatattt ctctaaaaaa ggccatctta taccagaaat aattcaaata
     3361 taagatagcc ttttattttt attttttaca cagtagtaat cttagttaaa gtataaaaat
     3421 ttaaagacta agccatttta ccgtggaatg ttctgtttat aacatctaat tgttgctctt
     3481 tagttaattt tatgaagtta actgcatatc cagatactct tatagttaat tgtggatatt
     3541 cttctggatg ttccatagca tcttctaaag tagctctatc aaatacattt acatttaagt
     3601 gatgagcatt ttgagcgaag tatccatcca tcattgcaga aagattgttt attctttcag
     3661 tcatatcttt tcctaaagca ccaggtacta tagagaaagt attagatata ccatcttgag
     3721 catgttcata tggtaattta gcaactgaag ataatgaagc taaagcacca ttagtatctc
     3781 taccatgcat tgggttagca ccaggagcaa atggagcacc agctcttcta ccatctggtg
     3841 tattaccagt tttcttacca taaacaacat ttgaagttat tgtaagtata gattgagttg
     3901 gataagaatt tctataagtt ttattctttc ttattttatt cataaagctt tcaactaact
     3961 ctacagctat actatcaact ctatcatcat tatttccgta ttttggataa tctccttcta
     4021 tttcaaaatc aacagctatt ccttgttcat ttctaatagt tttaacctta gcatgtttta
     4081 tagcagataa tgagtctgca caaactgata acccagccat accacaagcc atagttctaa
     4141 atatatctct atcatgtaat gccatttcta atgcttcata agaatattta tcatgcatat
     4201 aatgtattac atttaaagta tttacatata atgttgctaa ccaatcagtg aatggttcaa
     4261 atttactcat aacttcatca taatctaagt actcagaagt tataggttca aatcttggtc
     4321 caacttgaac accagatttc tcatctacac caccatttat agcgtataat aaagttttag
     4381 ctaagttaac tctagctcca aagaactgca tttgtttacc aattctcatt gcagatacac
     4441 aacaagctat accatagtca tctccccagt atgctctcat taaatcatca ttttcatact
     4501 gtactgaact tgtatctata gatactttag aacagaaatc tttgaatcct tgaggtaatt
     4561 tagttgacca tagaactgtt aagtttggtt ctggagaagg tcctaatgta tatagagtat
     4621 ttaacattct aaatgagttt ttagtaacta atgttctacc atctattccc atacctccta
     4681 tacactcagt tacccaagtt gggtctccag agaataggtt attgtaatca ggagttctta
     4741 ggaacttaac cattcttaat ttcataacaa aatggtccat atactcttgt aattcttctt
     4801 ctgttacgat tccagctttt aaatctcttt caaagtatat atctaagaaa gtagatgttc
     4861 tacctaaaga cattgcagca ccattttggt ctttaacagc acctaaataa ccaaagtata
     4921 accattgtac agcttctttt gagttagttg ctggttttga tatatcaaat ccataagatt
     4981 cagccatttt tttaagttca tttaaagctg atatttgctc agttatttct tctcttaatc
     5041 tgataacttc ctcatccatg caagaaactt ctaatgattt cttttgctca tttttatctt
     5101 ctattaaagc atcaacaccg tatagagcaa ctcttctgta gtcaccaatt attctacctc
     5161 taccataagc atctggaaga cctgttatta taccagattt tctagcagct ctcatttctg
     5221 gagtataaac atcaaaaact ccttggttgt gtgtttttct gtatttagta aatatatctt
     5281 ttatttctgg gtctaattca tatccaaaag cttcacatga attttcaacc atctttatac
     5341 caccatatgg catgacagct ctttttaaag gggcatccgt ttgtaaccca actattgttt
     5401 ctaattcttt atctaaatat cctggagcat atgcagctat tccagaaaca gtttttgtat
     5461 caacatctaa agtaccacca ttttctcttt cttttttaaa taggaccatt gcttcgtccc
     5521 ataatttttt agtattttca gtggcaccag ctaagaaaga gtcatttccc tcatatggag
     5581 aatagtttaa ttgtataaac tctctaacat ttatttcttt agtccatctt cctgttttaa
     5641 atccttgcca tgcattcatt aagtatttcc tccttagatt taaatgataa ataatactat
     5701 taaaattaat ttttatatac ttaatttatt ttatatattt ttttaaaaat atattttcta
     5761 atataataat agcatattat tcgtatcctg tatacataaa aaataaaaaa ttgtcgcttt
     5821 aaagtataaa acagattata tgttaataat gtggtgaaat tatacccata tataaataaa
     5881 ataaaacaaa taaatagcta attaaggaga aaatattttt tattttttgt ttaggataaa
     5941 taaattttat tatggaaaaa agaaaaagca tacatcttca caaactgaat atttatttaa
     6001 tagggggagg tgatagattg tttatcacag tggtattatt tttagtaggt ttcctattaa
     6061 ttactaaagg cgcagatatc tttataaatt gtactgtaga gataggtaaa aaaacgaata
     6121 tatcagaaat tatacttgga gctactatag ttagttttgc aacaacatta cctgagttta
     6181 cagtgtctct acttgcttct attgatggtc atactactat gagtttagga aatgcagttg
     6241 gttctataat atgtaataca gggttagcat tagggttggt tgtttttata agtccattta
     6301 atgtagataa aaaaatgttt ttttctaagt ctttactatt aatagtatcc gttatagttt
     6361 taattttatt gagtttagat ggagttataa caagaggtga ttcattatta cttataataa
     6421 tattaatctt ttatatggtt aataattata gaagtgtcgt tggaaaatct gatactaaaa
     6481 atagaaatat taaaaataac actattaaag acatatctac taaaaaaggg aaaaattata
     6541 gaggtttttc cgtattagaa attgtaaaaa tactattact ttttgctaca ggattgataa
     6601 tgatgataat aggttcacaa atccttatag agagtggagt aataatagct agttttttaa
     6661 atatacctca aggtatagta agtttaacta taatagcact tggaacttct ctgccagaga
     6721 tagtttcgtc gattactgct ataagaaaaa atcatcatga aatatcagta ggaaatattt
     6781 tgggtgcaaa catattgaat atagtatcag taatagctgt atcagcaata ccaaataaca
     6841 taccaatact atctcaaaat agacaattgg atataccatt catgatatta ttattattaa
     6901 tagtaataat accaacactt aaatctaata aacttagtag aatacaaggt atattgatgt
     6961 tattcacata ttttctgtac atatcaattt tgtatttcat gtatattatt taactatact
     7021 gtttaaaata ttaattgcag aatatcctaa aaaagtatat aatggatagg ttaagattaa
     7081 ttgataacgg agaggaataa tatatgaata taacaaagtt tgaagattta ccaataagtg
     7141 agggaataaa aaaagctata gctgaaatgg ggtttgaaga accatctcct atacaagcac
     7201 agtctatacc tgcgattttg tcaggtaaag acgttatagg acaagctcaa actggtacgg
     7261 gaaaaacagc tgcttttagt atccctatac tagaaacaat agacccaaat aatagaagtt
     7321 tacaagctgt agtgctttgt ccaacaagag aactggcaat acaagtatca acagaaataa
     7381 gaaagttagc taagtattcg cacggaataa agacattacc tatatatggt ggtcagccaa
     7441 tagatagaca aataaaatct ctaaagagtg gtgtgcaagt tgtaataggt acaccaggac
     7501 gtacaataga ccatatcaat cgtaaaacat taaagatgga taatgtaaaa atgattatat
     7561 tagatgaagc agatgaaatg ctagatatgg gatttagaga agatatagaa atgattttaa
     7621 gtaagattcc agaagaaaga caaacaactt tcttttcagc tactatgcca agaggaattt
     7681 tggaattaac taagagatat caaaaagacc cagagcatat aaaagttgtt agaaaagaac
     7741 ttacagtttc aaatacaaag caatactata tagaaactag gtcatcaaat aaattagaag
     7801 tattatgtag attagtagat gtatatgacc ctaaactatc tgttgttttc tgtaatacta
     7861 agagaaaagc agacgaattg gtaggagatt tacaagcaag aggatatttt gcagatgcat
     7921 tacatggtga cttaaaacag actcaaagag atatagtaat ggataagttt agaaatggta
     7981 caatagatat acttgttgct actgatgttg cagctagagg tatagatgtt gatgatgttg
     8041 aatgtgtatt caactatgac ttaccacaag atgaagaata ttacgttcat agaattggta
     8101 gaactggtcg tgctggtaga gaaggtatgt catttacttt tgtatttgga aaagaaatga
     8161 gaaagatgaa agatatagaa agatatacta aatctaaact aataaagcat aatataccaa
     8221 caataactga tgttgaagag aaaaaagtag gtactttctt tgcacaagtt aaacaaacta
     8281 tagaagaagg tcacttaact aaacaattac aatggttaga aggtttctgt aatgatgaag
     8341 attatgcaat ggttgatata gctgctgcac ttgtaaaact atctctaggt gaagaaatga
     8401 aagaagaaat aatagaagaa aaacctagaa gagagcgtgg agatagaaaa ggtggtactg
     8461 gagctaaaga tggtatgatt agattattta tcaatattgg tagaaatcaa agagtacaag
     8521 caaaagatat agttggtgcc atagctggag aagttggaat accaggtaaa gtagtaggta
     8581 ctatagatat atatgataag tatacttttg ttgaaatacc taaaaaagat gcaaaaacag
     8641 taattgaaaa gatgaaagat attaagataa aaggtaataa gattaatata gaaaaagcaa
     8701 ataagaagaa aaaataacgt atagtataca aaactataat tagaaaatat ataaaataat
     8761 atattggata tttaaaaagc ctgatgtgtt ttcaaaacag atagtagata atctgtaaga
     8821 gttaacagca taaggctttt cttgttttat atgatgttat aattttccag attgttgtga
     8881 aaaatgtata ttagttcctt tttttcaaat ctgatttgaa aaagagaata ttaattatct
     8941 atatagttta gattataata aaaaatgtaa gataacaaag gggagaaaaa gatgtcaatg
     9001 acttatatgg accctagggt ttttttgatt ttagaaatgg tgttagaaga agacacaaat
     9061 actctagaac aattttcaaa tgttttgggt gttagtacta ggactatcag aaactatata
     9121 aaccagatta atcgagaatt aaaaggtggt attgctaaaa tagttaaaaa tgaaaaaggt
     9181 acatatagta taagaattga ggatgaaagt aaattaattg agattgtaaa ttttaataga
     9241 ggaaagctat caaattttat aaatttaaat tcacctgatg aaagaataaa ttatgttctt
     9301 gatattttaa ctatgaccaa caatccaatt accatagatg atttggctga agagatatgt
     9361 ataggaagaa caacattaat aaaggattta aaaaaagcag acagaatttt agctgaatat
     9421 gatttagaat taaaaaggaa gccaaatata ggtatgattt tagttggtga agaaatggat
     9481 ataagattat taatccttga tagattgtat gaaaattata ttgatgtctt ggaaaacgtt
     9541 aatcagataa atttagttaa agattatgat attgaatgtc ttagagatga attaaaaaag
     9601 ttatttaaaa aagaagaact ctacataaca gaacaggtct tgagagatgt tgaaagatat
     9661 ataatcatgt ctgtcttaag aaatctaaat gggtacaaat ttgaaaagat agataagaga
     9721 tttgaagtga taagatgttc agatgaatat actttaggat taaagctcaa agctttattg
     9781 gaaaagatat ttaatatagt tttaaatgaa aaagaaacca tatttttgac aatgccatta
     9841 ataggtagaa aagctcctag tacaaaatta gcacttagca gtataaagat aaatgatagc
     9901 gtaaaggaag ttgtagatga agtaactagt tttttattag agacctcagg gattgatttt
     9961 aaagaagata aaaagcttat agaaaatttg gaatatcact tgtattttgc acttaataga
    10021 atgagattca acataagggt aaaaaatcct cttttacaag aaataaagga aagatataca
    10081 tttccatata gtgttgcaaa gatagctgca atgataattg aggaaaagtt tgacttgaaa
    10141 atatgtgacc acgaaatagg atatatagca ttacattttg gaagttattt tgaaaaaaat
    10201 tcaagaaaag ttctaaatgt aaatagggta gctgttgttt gtggaacagg tcttgggact
    10261 gcacaactta ttagaataaa gataggaaaa atacttggag aaagtgtaga agtaaatact
    10321 ttttctgata tagaagctaa agctaattta gatttattaa agtattatga tttagtattt
    10381 acaacagtag atttcaaaac agacttagat gttccaattt ttagagtaaa tgcgttattt
    10441 aatgaagaaa gtataaagaa agagatagaa aatgcaatac ttttgaaaaa tagtataaat
    10501 cttaagtcta taaaaaagat tgaaaaaaat atccctttta taggtaaatt aattgaagag
    10561 gataagttct ttatattgaa taagagtacg tttatggaaa atttagaaga tatgttagaa
    10621 aaattagttc ataaaggaat tattgatata gagttcaaaa aaaaggtatt aaaaagagaa
    10681 aagaaatctt caacagcatt agataattat atagcattgc ctcactcagt taatactaat
    10741 ggggaaaatc tttatctggc atttggaata ttagaaaaac cagtgatttg ggatacaaag
    10801 gaaattagaa taataatatt gatgataatt ccagataaga atgtagatag tacagacctg
    10861 ataataaaaa gttatgaaga agtacttgaa gtagggagag ataaaaagat ggtagaagaa
    10921 cttgctaagg caaaatcatt tgatgaattt tataaaataa ttaccaagag gaggtaataa
    10981 tatggaccca gtaattttta ttatagcatc acttattaca gtgtatttat taaatatttt
    11041 tcttggatac ttacagctta aagattttaa taaaaattat attgaactaa aaagaaaagg
    11101 aagagtggca attggcagaa aaaaaggaag aatttcatcg ggtacaattg tgttgatact
    11161 tataaatgat gatggtgtga ttgtcgagac tagaaaaatg caaggtgtta cagtattggc
    11221 aagagtaaag tcatttgaag gtttggtagg aaagaactta gagtatatag aaaaatcaga
    11281 tttaactgtt tacaataaac tacttaaaaa agcaatacta gatgcagtca aacagtacat
    11341 aacatttgag aaaaataaat gtgaagatac aaataatctg agtactgatg aagagtgtag
    11401 gatggaagta gtttaaaaaa atatatttat aaggaggagt taatatgacg caattttttc
    11461 aagtattagc aagtggtgca gatagtttta tgaatttatt tagggcaggt gggcaacaat
    11521 ttgtaggttt tgtaacagat atagtaccac ttttagtagc attattagtt acaatgaatg
    11581 caattatcaa ctttattggt acagatagag tcgagagact tgcaaaaaaa tgttctgcaa
    11641 atatatttac tagatatttg atattaccag tccttggtac atttgtattt gcaaacccaa
    11701 tgacactatc attaggaaga ttcttgccag aaaagtttaa acctagttac tttgcagcag
    11761 caagcttttc atgccataca atgaatggat tattcccaca tatcaatcca ggtgaactat
    11821 ttatatatct aggaattgca aatggtataa caactttggg attctcaaca gcagatttag
    11881 cagttagata tttgttagtg ggtatagttg caaactttat taaaggtgta gttactgact
    11941 atacaacaaa atttgtagag aaacaacaag gaatagcatt aaattcagat ataaagattg
    12001 gataattact ttaaggtata aataaatttt tcaaataaaa agatagtgta tagaggagga
    12061 taaattatat ggcatttaac aaagttaaaa tagtaaaagg ttctggagga tggggtggac
    12121 cattgataat agaaccaaca gaaaagaaga ataaggtagt atatgtgact gggggagcaa
    12181 agccagaaac tgcagtaaaa atagctgaac ttacaggatg tgagttaata gatggatttt
    12241 cacagggtgt taaagatgat gaaatcgcat gtgtaataat aaattgtggt ggaacattga
    12301 gatgtggtat atatcctcaa aagaaaatac cgactataaa tataatgaaa acaggaaaaa
    12361 gtggaccatt agcaatgttt ataaaagaag atatatatgt ttcaggggta aaaccaacaa
    12421 atgtagagca gttataattg aggtagtagg agggaagaaa tatggcttat gatactaaaa
    12481 agaagataag tgagcagagt caaggaattg ttgcaaaaat aggtatagca ctaggaaatg
    12541 tgacaagtac tctttttcaa gctggtaggg atactataga tacattaata catactatac
    12601 tgccttttat ggctttcgtt gcagtcctaa taggtataat aaatcaatca ggtataggaa
    12661 attgggtagc caatttactt actccattaa tcggaaatgt tggaggttta gttgtcttat
    12721 caataatatg ttcatttccg ttgttgtctc cgtttcttgg accaggagct gttatagctc
    12781 agatagtggg agtattggtt ggtacagaaa taggaaaagg aaatattcca ccaagtttag
    12841 cattaccagc attgtttgca ataaactcac aagcagcttg tgactttata ccagtaggtc
    12901 ttggtcttgc agaagctgaa cctgatacag ttcaagtagg agttccgtca gttttatatt
    12961 ctagattttt aacaggtgca ccaactgttc tattagcatg gttagcaagc tttgggttat
    13021 ataaatagaa gaatacttta taaataatta gagagaggtt cagttgatat ggaattaata
    13081 tacagtactc aaataaataa aataggagat aatgcaaatg aatttttaca acataatatg
    13141 tttgtaactt tcaaagataa tgctcctcaa gagttagaaa attactgtta tatacattct
    13201 gaaaataatt tagtaaaaga aatacttacg gatgatgtgc tatcaataaa taatgttgat
    13261 tacaaaatta cttctgttgg tgagttagta aaccaaaatc tttcagaatt aggtcatata
    13321 acatttaagt ttacaggtga aaaagatgga gcaattggag gaaccttata tcttgaagaa
    13381 aaagaaatag cacctattgg cgtaggtact atactaaaaa taattagaaa ataaaattta
    13441 aaacgaatct aatcatatta taagtaaatt atagataaaa gtcaatttaa ctaatcaagt
    13501 taacttttaa tgtgataaaa aaggggaaat gaaaatgaga aaagtggctt tagtcgttgg
    13561 aggaggaaga agtttaggtg aatatttatc taaacattta gctaaagtag gttatgatgt
    13621 tgctattgca gatttaaatg aagacaacgc aaaaaaagta gctgaagaaa taaaaactga
    13681 atttggttgt aaatctgtag gatatagttg taatgccata gatgaaagac aggttataag
    13741 tactgtcaaa gctattgaaa atgattttga tagaatagat ttattagtgt acaatgcagg
    13801 agtggctgta agtaaaaaaa taactgattt tggatacaaa gattttaaat ttattgtaga
    13861 cgtaaattta aatgggtact ttctatttgc aagagaagta tctaaagtca tgatagcaaa
    13921 aggtattgag ggtagaataa tacagataaa ttcaaaatca ggtagagtgg gaagcaagca
    13981 taattcagga tattcagcag gtaaatttgc tggagtagga cttactcaaa gtttagcgct
    14041 agatttggca gaacataaga taactgtaaa tgcactgatg ctcggtaatt tacttgattc
    14101 tgatatgttt gaaagtttaa ttcctcaata tgcagtaaaa ttgggaatac ctgaatcaga
    14161 agtcaagcaa gtttatatag ataaagtacc tttaaaaaga gggtgtagat ttatagatgt
    14221 tgcaaatatg cttacatttt atgcttcaaa ggaagctgaa tactgcactg gtcaatctat
    14281 aaatattact ggtggacaag ttatgtaagg tttttatgga gtgtctcaag atgaactctt
    14341 tagttcatga ggcacttttt tgtataaaat taaatatatt ttcagcattt aaacaatgaa
    14401 aaagaggttt atctttattt tgagatagcc tcatttttgt aattaaaaaa catagtgttt
    14461 aagtaaacat ataagattgt tagtttttta aaagagaatt ttttaataat attatattta
    14521 tgaagtattt ttaaacttat tttgataaaa tatagctatg ttttatatac tgtagttttg
    14581 aacatagaaa gtttaataaa ttatagtata tatgttaatt tttaataagg taagaaagta
    14641 gaaagaagga tattttatgg atataataaa aaaattaagt catgggtgta cattagattg
    14701 tcatgactgt tgtaaattca atgtttatac taaaggaaat aatgtagtaa aaattgaagg
    14761 agataaaaat catccttata caaaaggttt tatatgcaaa aaagggatgg cccatttaga
    14821 tagattaaat cataaagata gaataaagac tcctatgctt aaagttgatg gagtatggaa
    14881 agagatttct tttgataaag ctatagaaat aatggcagaa aagcttacat actataaaga
    14941 aaagtataca tcaaagtctg tcatgcatta tgaccaatat ggaagtgggt ctgtgctaaa
    15001 atacatagga gatatatttt ttaattttta tggaggtgtt agcagacata aaggtggtcc
    15061 ctgttggagt gcagggatgc atgctcaaaa atatgatttt ggagtagcta agagtcatgc
    15121 tatagaagat atgttaaata gtaaaagtat atttgtgtgg ggaaaaaatc cagcatatac
    15181 tacaattcat actatgcaaa taattaaaaa agctaaagaa aaagggataa agatagtagt
    15241 gattgacccc atatatacaa aaacagctca aatagcagat aagtatgtac aagtaaatcc
    15301 tggaacagat ggagctttag ctatagctat ggcaaaaatt atagtagaag ataaattata
    15361 tgatgaagaa tatataaact cgtatgttat tggttttgaa gaatataaaa aatatttatc
    15421 ttcattggaa ttaagttttt taattgatga atgtggtgtt aaagagaatg atataagaga
    15481 gttagtggat ttatatacta acaaatattc aagtataaat gttggatatg ggcttcaaaa
    15541 atataaaaat ggtggaaaca caataagagc aatagatgct ttaggtgcta taactggtca
    15601 aataggattt agtggtggag gggtaaacta tgctaataaa gtttatccga gtgttataaa
    15661 ttctgaccct tacaatagcc aatcatatgg tgaagatagg gagttttatg taagtaatat
    15721 cagtaaattt atagaagaat ctttaaaaaa tacatcaaat aaagtaaatt atgcatcaga
    15781 tgaacttgat atgacatcaa ataaagtaaa ttatgtatca gatgaacttg atataacatc
    15841 aaataaaaca gattatatat caaatgaatt gtataattta tctaataagt caatcaaagg
    15901 taatattcct ataaagatgg ctgtaataac taaaagtaat atgctgaatc aactgccaga
    15961 tttagtagaa ttggagaggg tgttttcaaa aattgaattt aaagtatgtt ttgatatgtt
    16021 tataactgat acagctactt tatgtgacat gtttataccg tgtacaaata cactagagag
    16081 tgaagatata atttatagct caatgaccaa tccatatata acctataatg aaagagctgt
    16141 aaagccagca cataaattga tggatgaata ttacttcttt atggagctag ctaaaaagat
    16201 gggattaaat gattatcctt ttgtagaaaa gagaacttat ttagaaaaag tgattgaacc
    16261 acttaagcgt tttgacaaaa atctggatat agaaaaactt aaaaacaatt actttactat
    16321 acataaccct gttgcttggg aagataagaa gtttgagact ccatctggaa aatacgagct
    16381 ttattcagaa agtataaaaa acttgggaat aagtccaaca cctgtatata taagcaataa
    16441 atataaagaa atagaagata aaaatatttc ttttagatta ttaactaatc atcatgcaga
    16501 tactttattt agtcagcatt ttatggacaa aaaatctata gctcaagcat atataaatcg
    16561 gagaatggct aaaaaagtag gtattgagga caaagatatt gtgatattga ggtcaaaaaa
    16621 agcaaaaata aatgtacaaa taaatataga tgatggagta ggtaattata tagtaaaaat
    16681 gtatgttggt tggtggaaga aacatggaaa tcctaatagt ttaacagaca ctggtatatc
    16741 agattttggg ggtcaggtaa catataatga aagtatggta gaaataatac gacaaaacta
    16801 gttaaatttc attgaaggaa taaaaatata attatagaat tgattaaaag ttactatttt
    16861 tacaaaacta ttgaaggagg gatttttgat ggtaaattat tccttagaac ataagaatct
    16921 atacatagaa tttatgtgct cagaactaga ccaccatgta gccaatgaaa taagggaaga
    16981 aatagataac ttattaagtg taaatcaagt aaaaaatgtt gtatttaatt ttgaaaatat
    17041 taattttatg gattcttcag gtataggcgt catcataggt agatataaaa agatttctaa
    17101 tgaaggggga cgagtttcag taattaatat aagttcacga gttaagaaga tttttgattt
    17161 atctgggtta aataaaatta ttggcattta tgatacgtat gaagaagctc taagttcttt
    17221 gtagggagga gtatgtgatg aataatatta tggaagttaa attttcggca atatctgaaa
    17281 atgaaagttt agcaagagtt atagtagcat cctttgcagc taagttagac ccaacagtag
    17341 atgagttagt agatataaaa actgctgtct ctgaggctgt tacgaatgct ataatacatg
    17401 gatatgaaga agacagttct aagtttgttt tcttaagatg tgagatagaa ggaaatacta
    17461 taaagattat agttgaagat gaagggtatg gaatagaaaa tgtggagaag gcaatggaac
    17521 cactttatac atcaaaacca gaacttgata gatcaggaat gggattcaca gtaatgaaaa
    17581 gctttatgga cgatgtagag gtaagttctg taaaagataa tgggactaga attgaaatga
    17641 ctaaaaaaat aaatgtgcct aaataaagtc caaaagtgag gttttattat ggaagtaact
    17701 gttgccagag aagaaaaaaa acctcttctt agtcatgaag agactctaga acttattgaa
    17761 aaagttcaaa atggagatga agaggcaaaa gaaatattaa tatcaagcaa tttaggtcta
    17821 gttaggagcg ttgtatctag atttttaaat ataggatatg atagagaaga tttatttcag
    17881 ttaggttcta taggcttaat aaaatcaata tataaatttg acccaaaatt taatgtaaaa
    17941 ttctctactt atgcagtacc aatgatttta ggtgaaataa agagatactt aagagatgat
    18001 ggtatgataa aagtatcaag gtcgttaaag caaatagcta taaaggcaaa gatggaaagt
    18061 gaagctttaa ctaaaaaatt aggtagagag ccgagtatag aagaattagc taaagcaata
    18121 gatgtagaaa aagaagattt agtaatggct atggaagcta atttcaatgt tgagtatttg
    18181 caaggagtta ttcatgaaga ggaaggttct ccaatatgcc ttattgataa aataagcatg
    18241 aaaggagaaa gtgaagaaga aaaagtagtg gataacatat tgttaaaaga catacttgga
    18301 agactggata agagagagcg tcaaataata gttctcagat actttgaaga catgactcaa
    18361 agtgaaatag gagagatgct caatatatca caagttcaag tatctaggat agagaagaaa
    18421 gtattgtcta agttaaaaga atatatatcg tgataatact tatgatatgt agaataataa
    18481 aatataataa atatattact aaaataaaat gtaataactt tttatgttaa gtttttaaat
    18541 tattaaaaga tattaaaata ataaaaagca ttaaaaaagt aaaaatataa aaacaaggtg
    18601 tctttatgga aaaatagatt ttttctttat taggcatctt ttttattttt tgaccaaact
    18661 ttagatagtc aaaataaata ataggtttat ttttttaaaa ttaacaaata atactttaga
    18721 gaggtgttta gtatggataa aaattataaa aaatatgtag accaaataag cccaaaacct
    18781 acatatttaa aaaactatac acttgcattt attgtaggtg gaattatttg tatgataggg
    18841 caagcaataa atgatttgta tatgaaagtt ggaggtcttg ataaactagg tgctagttct
    18901 gcaacatcaa taacattaat atttataggg gcatttttaa ctggattagg agtttacgac
    18961 ctaataggaa aaagagctgg agctggttct ataattccaa taacaggttt tgctaactca
    19021 atagtatctc cagcaatgga gtataaaaga gaaggctatg tattaggtgt aggtgcaaac
    19081 ttatttaaaa ttgcaggacc agttttagtt tatgggatag gttcttctat tttatgtgga
    19141 attatttatt atatttttaa gatgttttag atgattttta tttaatttaa attttattaa
    19201 ttatgaagaa ggtataaaat ctgaaaatag ctactaaagt aactaattga aaaataattc
    19261 tgcagattta attaatattt ttaattaata ttaaaaattt gaatagtaaa atgcaaaatt
    19321 aaaaaggtgt gaaaatatga aaaataaaag aattggaaaa agaacagtca agttagaaaa
    19381 taaaccaacc ataatatcaa ctggaactat agtaggtcct aaggaaggtg aaggaccact
    19441 aaaggactat tttgatatga ttatgactga cgatttatat ggtgaaaaga cttgggaatt
    19501 agctgaaagt aaaatggtag agactgcatc tcaacaagct atccaaaagg caggtaaaaa
    19561 attgtcagat gtaaattata tgctaggtgg cgatttaata aatcaaatag ttccagcatc
    19621 ctttgcagca agagaactgg ctattccatt tttaggtata tatggagctt gttctacaat
    19681 ggcagaaggt ctttgtatag gttctatgct tatagatggt ggttttgctg atttagtact
    19741 gtcaggtaca tcaagtcact attgtacagc agaaagacaa tttagattcc cacttgaact
    19801 tggaaatcaa aaaccaatga cagcccagtg gacagttaca ggagcaggaa gtgtattatt
    19861 agctcctaat ggagacggtc caaaagtaaa gtatgttact gttggtaaag taatagatga
    19921 gggtattgat gatggtaata atatgggacc agctatggct ccagcagcaa tagatactat
    19981 ctactcttac tttgaagata ctaatgatga cccaaatagc tttgatataa ttgctactgg
    20041 agatttaggt actcttggaa aacagatagc agaagacttt ctaaaagaaa aaggtgtaga
    20101 tatatcaaaa gtttatactg actgtggtat agagatgttt aaccttaaag aacaagatgt
    20161 ccactgtggt ggaagtggat gtgggtgttc agctacagtg tttgcaggat atatatatga
    20221 taagttgaga aaaaaagaat ttaacaaagt aatgcttgta tcaacaggag cactactttc
    20281 accgacaagt acactacaaa agcagacaat accatgtgtt gcacatgcag tagtaatagt
    20341 aaatgagtag gaagtgatat aatatgttaa tggactatgt aagagtattt attgtaggtg
    20401 gaattatatg tttaatagcc caagtaatga tggattattt caaaatgcaa actccatatg
    20461 ttatggtaac gtatgtaaca actggtgtag ttttaacttt tctaggactt tatgagccat
    20521 tagttaagtt tgcagcagca ggtgcaacag ttcctataat aggatttgga tattcacttg
    20581 ctacaggagt aataaaatca attcaaagtg atggtttttt aggtattttt ttaggaggta
    20641 caactgctac agctggtgga atagcagcag ctatattctt tggatatatt atgtcagtag
    20701 tatttactcc aaaagcaaaa ccataaatct ttattaaaag aataataagt ttttgaaata
    20761 ataacttttt ataaagcagg tggatatctt ttagattcca cctgtttttt atataaagaa
    20821 gcgtttacat aattgaagtt gacaattatt tacaacatag tgtagtatta tattatcaaa
    20881 ataaaatgaa gtgaagtagg ggataaagat ggtaactatt aaagatattg caaggaatct
    20941 aggtatcagt tattcaactg tttctaggtg cttaaacaat aatcctaatg tatctgaaaa
    21001 aacaaaaaag aaagtggtag aagaggcaaa tagactaggg ttccatttta atgtaaatgc
    21061 aagaaattta gcaaaaaaag aaactaacag gataggggta atcttttcaa ataattttaa
    21121 tcatcaagat actagaaagt tttttagtga tataatggat agttctataa attctattga
    21181 gactaataaa tacgatttta taatacagcc taataataac attagtggtg atagtaatgt
    21241 atataaaatg gtaaatggac aaatggttga cggattagta attgtgtctc aatcaataaa
    21301 aaaagaagag tatgattttt taaaagacaa taattttcca catgtattta tatattttaa
    21361 accatctttt ataggagaag tagataattt cttttgggac gataacgtgt atggaggtta
    21421 tatagctacc aagcatttaa ttgaccatgg tcataaagat ataataacag taacttcaga
    21481 tgataagagt tcaaaaatgc atgaagatag aaccaaggga tatttgaatg ctatggaaga
    21541 agcaaattta aagacagaag tcattaaatc taaaatggat tttgaatctc aagttgaatt
    21601 tttagaaaaa tacattgata aaataaaaaa agcttcagct atatttgtac aacaagatgt
    21661 accagcgatt tctataatac aagagttaaa aactacttat ggtataaatg taccagaaga
    21721 tatttccata atagggtata ataatattga attaatatca tattttaggt cgcatttatc
    21781 aacaatagat gacccaagag agcaagttat aaaaaatgga gtagatagtt tagtaaatat
    21841 aataaataaa aagagtacag aacacattcc aagaaaatta tatccaaggt taataatacg
    21901 aaatagcgtt aaaagaataa agtagtaagc ttttaagtat caaacgttta ttaaaaatat
    21961 tatagattat gtgaagtttt ttaacaaata atataaataa aaattaaaag gagtaggtgt
    22021 atataagtac attgtactcc tttatttatt gagatagttt ttataatatg aatgaataat
    22081 tttattcaat tatttgaaaa tccgttattt taacaatata aattaataaa ttatttgtat
    22141 tttatatcat gaatctataa tatttttttg attcataact aaaagtatat aaaaatgatg
    22201 aataaatata tattatacat tatcaatgat aggttatata ataaaatcac taacaattat
    22261 attataagaa aattataaaa attacaagga ggtatagaaa tgaaagaaaa agcagggatt
    22321 agaaatgcca taatagcagc ctttataatc acaatattgg cagaattaat tggacctatt
    22381 tcatttaaag ttatgggaat taatgtaact ttacttagta tactatgggc tatattcata
    22441 ggaatggctg tttctcctca tttattaggg agagttatac cagccttaaa aaagtttata
    22501 ggggataatg aaataaatgt atctccatat ttattatctt tgacactata tccattaggg
    22561 ataatgtttg gaattaatgc agggcctaaa gttggtatat tacttcaagc aggaccagca
    22621 cttttattac aagaatttgg tcatatgggt acaatgttga tagcattacc tgttggagta
    22681 gcattaggtc ttggaagaag tgcattaggt ggtacttttt cattatgtcg tgacacagca
    22741 ctaggaatta ttggagataa gtatggatta aactcaccag aaggtatagg aacactagga
    22801 acttacataa gtggtagtat atttggaaca ttattatttt catttttagc accagtgggt
    22861 gtacttttag gatttcatcc atatgcttta gcaatggcat caggaatggg tagtggttca
    22921 atgatgggag ctgcaacagc atcattaatg aatacagttc cacaaatgtc agagcagatt
    22981 ttagcatatt cagctactag tggattgatt acagctgtta ctggaatata tatagaatta
    23041 ttcttatctt taccagtagc caactactat tatagcaaag tagctccagt tattgaaaga
    23101 tttagaggaa gaaaaacaag aagaacaagt aataaattgt aaaggaggat atatatatgg
    23161 aaaaaacttt taagactatt atggagcaga taaaaatagt attgtttata tatattatta
    23221 tttgctttgg acaaatgata gcacttaaga ctcaggctca agatttgttg attggaagtg
    23281 tatctggatt tttgttggtg ataattgcta ttataataaa gaattttgtt aaaaaaccta
    23341 atttaccagg ttttgcatgg gctacattag ttgcatttgt tttatcgctt ccaatatctc
    23401 cagtggggga tatcatagca acaaatgtag ggaaaatcaa ttttatgtct acagtaacac
    23461 cattattagc ttttgcagga atctctgtag gtaatcaatt agatatatta aagactttga
    23521 gttggaagtt agtagttata tcatttatag ttatgacatc tacttacttt ggttctgctt
    23581 ttatatctca aacagtttta agattaaatg gaatgcttta aggaggtagt aaatatgaat
    23641 gaattaaaag caaaaattaa agaggaaata aagttattat ctgaagagaa aaagacatca
    23701 tttgaaaaag tttctgacta tttattttca aatccagaat tagcatttga ggagtataag
    23761 tcacaaaaag cattatgtga tttgttagaa gaaaatgaat ttaatgtaac taaaggtgta
    23821 ggaggattag aaacatcttt tgaagctgtg tattcaaatg gaactaatgg gaaaacagtt
    23881 gcttttttag ctgaatatga tgcacttcca ggaatgggac atgcatgtgg gcacaatatc
    23941 atagggacaa gttctgttgg tgcaggtatc atattaaaag aaatcatgaa aaaacataat
    24001 atagaaggga ctgtaaaggt ttttggaaca cctgcagaag aaagagtagg tggaaaaata
    24061 actatgatta aagaaggtgt atttaataat gtagatgctg cattaatttt acatccaagt
    24121 gatgcttcaa tgcctgatga tatatcattt gctcaagtta atttaaaatt tgattttact
    24181 ggtaaggcat cacatgctgc tgcttttcct tgggaaggga aaagtgcctt aagtggagta
    24241 atagcactat ttaacagtgt aaatagcatg agattacatc ttaaagatta tgcaagagta
    24301 catggaatta taactgatgg aggtagcata cataatataa ttcctgaaaa atctacagct
    24361 atatttaatg taagagcctt aagtatagaa tatttaaatg agatttgtga aatgcttaaa
    24421 aattgtgcta aaggagctgc aatttctact ggaactaatg tagaaatagt tcaattagat
    24481 gaaatataca aagagattaa gaatgattct gaattagtga atatagttag acaaaatttt
    24541 gaagttttgg gtgaagacta tgttgaaaga gatttaagtc aaggtatagg ttcaactgat
    24601 acaggaaatt taactcatga gatacctgca atacaagcat atataaaatt aaaagaaaat
    24661 actgcaactc atacagatga atttgctgtt gcagctggtg gtgaagaagg cagagtagca
    24721 cttataaaag caattaaggt gttagcaatg tgtggagtag atattttgta tagtaaataa
    24781 tatataattt taaaatatat taagaaagga aatcggatta gacatgatat taaaacaagt
    24841 aatagaggtt tatgacattt tagataaagc aaatgctaat ggggaagaag taaaaactta
    24901 cttacaaggt tatggtgaag tagatgtaac tgtaaaagaa ttgtcttcaa gtaaaggttc
    24961 aacagattta gtaaagctta caattcctgg aaaaaatgga aaattaaaag gtggagatgc
    25021 tcctactctt ggtattttag gtagactagg agggattgga gcaagaccag aagttatagg
    25081 ttttgtctca gatggtgatg gagcattggt agcaattgca gtggctgcta aacttttgga
    25141 tatgcaacga aaaggggata tattagatgg tgatgttgta atttcaactc atatttgccc
    25201 agatgctcca actaaagaac actatcctac tccttttatg gattctccaa ttgatatgat
    25261 gactatgaat gaaaatgaag ttgatagtag ttgtgatgca atactttcta tagatactac
    25321 taaaggaaat agagtaatca atactagagg atttgcaata tcgccaactg ttaaagaagg
    25381 ctacatatta aaaactagtg ataacctttt agatattatg cagactgtaa cagggaaatc
    25441 tccatttgta ttccctttgt ctattcaaga tataacacca tatggaaata atctgtatca
    25501 cttaaatagt attttacaac cagcagtagc tacagatgca ccaatagtag gagtagcaat
    25561 tacaacagag atgcctgttg ctggatgtgc tacaggtgca actaatttta gtgatttaga
    25621 ttcagctggg agatttgcaa ttgaagttgc caagctattt ggtagaaata agtgtgattt
    25681 ctatgataaa gaagagtggg aaatgcttat taagagatat ggcaaattaa atagatttca
    25741 aacatttggt atacagtaat tagaaaaata ttaagtttat ataatttgtg atataactat
    25801 ttaagaatat ataaacaatt aggaataaaa gtaagaataa agcacttagt taaaaacttt
    25861 aaaaataaaa tatttatttg tttatataaa aaggaggatt ctggttattg ggattctctt
    25921 ttttatgaat acaaataata tattgtaatt ataaaaaaag gagcgtttca aagtgatttt
    25981 tttaatcacc ttgagacaac tccttatatt tttatttaaa ttctattttt tattaagttg
    26041 ctggagttcc accaccatca ggaggagtag tagagccacc accattattt ccaccacc
//
LOCUS       DAYATN010000043        25587 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.043, whole genome shotgun sequence.
ACCESSION   DAYATN010000043 DAYATN010000000
VERSION     DAYATN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.043"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..984)
                     /locus_tag="ACGI8A_001726"
     CDS             complement(<1..984)
                     /locus_tag="ACGI8A_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFH0179724.1"
                     /translation="MFILKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVVYDEKTKNI
                     DKFKSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYT
                     FGNLDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLE
                     FVNLEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCF
                     NKFKESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKL
                     KDIEEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDN"
     gene            complement(1023..1883)
                     /locus_tag="ACGI8A_001727"
     CDS             complement(1023..1883)
                     /locus_tag="ACGI8A_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFH0179725.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2152..2361
                     /locus_tag="ACGI8A_001728"
     CDS             2152..2361
                     /locus_tag="ACGI8A_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HFH0179726.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2361..2627
                     /locus_tag="ACGI8A_001729"
     CDS             2361..2627
                     /locus_tag="ACGI8A_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HFH0179727.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2656..3228
                     /locus_tag="ACGI8A_001730"
     CDS             2656..3228
                     /locus_tag="ACGI8A_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFH0179728.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3463..4653)
                     /locus_tag="ACGI8A_001731"
     CDS             complement(3463..4653)
                     /locus_tag="ACGI8A_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFH0179729.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4913..5344)
                     /gene="dut"
                     /locus_tag="ACGI8A_001732"
     CDS             complement(4913..5344)
                     /gene="dut"
                     /locus_tag="ACGI8A_001732"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFH0179730.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5457..7685
                     /locus_tag="ACGI8A_001733"
     CDS             5457..7685
                     /locus_tag="ACGI8A_001733"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFH0179731.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7796..8281)
                     /locus_tag="ACGI8A_001734"
     CDS             complement(7796..8281)
                     /locus_tag="ACGI8A_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFH0179732.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8533..9756
                     /locus_tag="ACGI8A_001735"
     CDS             8533..9756
                     /locus_tag="ACGI8A_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFH0179733.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPSILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9760..12894
                     /locus_tag="ACGI8A_001736"
     CDS             9760..12894
                     /locus_tag="ACGI8A_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFH0179734.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12916..14226
                     /locus_tag="ACGI8A_001737"
     CDS             12916..14226
                     /locus_tag="ACGI8A_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFH0179735.1"
                     /translation="MNKKMSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSD
                     SNKRTSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFP
                     YDQIGLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMK
                     LKKDLGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEY
                     RFESFRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVA
                     PTKPEQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFN
                     TENGLVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGL
                     ITLTDYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14515..14760)
                     /locus_tag="ACGI8A_001738"
     CDS             complement(14515..14760)
                     /locus_tag="ACGI8A_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFH0179736.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(14974..17601)
                     /gene="ppdK"
                     /locus_tag="ACGI8A_001739"
     CDS             complement(14974..17601)
                     /gene="ppdK"
                     /locus_tag="ACGI8A_001739"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HFH0179737.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17662..18504)
                     /locus_tag="ACGI8A_001740"
     CDS             complement(17662..18504)
                     /locus_tag="ACGI8A_001740"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFH0179738.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18511..19149)
                     /locus_tag="ACGI8A_001741"
     CDS             complement(18511..19149)
                     /locus_tag="ACGI8A_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0179739.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(19585..20877)
                     /locus_tag="ACGI8A_001742"
     CDS             complement(19585..20877)
                     /locus_tag="ACGI8A_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFH0179740.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDIDLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(20914..21270)
                     /locus_tag="ACGI8A_001743"
     CDS             complement(20914..21270)
                     /locus_tag="ACGI8A_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFH0179741.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(21305..21664)
                     /locus_tag="ACGI8A_001744"
     CDS             complement(21305..21664)
                     /locus_tag="ACGI8A_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFH0179742.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(21733..23706)
                     /locus_tag="ACGI8A_001745"
     CDS             complement(21733..23706)
                     /locus_tag="ACGI8A_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0179743.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(23928..24941)
                     /locus_tag="ACGI8A_001746"
     CDS             complement(23928..24941)
                     /locus_tag="ACGI8A_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFH0179744.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(24954..25499)
                     /locus_tag="ACGI8A_001747"
     CDS             complement(24954..25499)
                     /locus_tag="ACGI8A_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFH0179745.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
BASE COUNT         8753 a         4268 c         3263 g         9303 t
ORIGIN      
        1 attatctaag tcttcatcaa acatttttat tatatttttt atctcatact tgtactcatc
       61 ttcattcatt ctataaagca ttaaatcttc tagtacatct ttcatgtcga agtacacatc
      121 ttctaataaa taaaaatagc cttcctctat atctttcaat ttatctatag tatccatttt
      181 tttacttata tctttcataa tttctaaatt ttgtatattt acatctatga ttgttaataa
      241 gtacattctc acagttttta ttatttcaac agaaaaatct acaatattat aatttccttc
      301 ttttttatat aatgactcct taaatttgtt aaaacaatta tctaaattag aatttatatc
      361 gttaaatggt ggtcttatta tgtcacgtat tattaaaaga ttttgtatat ctacattatt
      421 tgtgattgcc tcatccaaaa taaataaatt atatgcatca tttaatgggt catgggcatc
      481 tccactaaac tctaagttaa caaattcaag taaatttttc aatgatatat agttcataca
      541 tctcatacca caatttatat atttttcttt tatatctaca aataaatctc taatgttatt
      601 tacaaacctt ggatggttat tttttttagc acttctcata tctgtgttat tgaaacatgt
      661 caaatccaaa ttaccaaaag tatatattcc tttaatatca gaaaatatgc caagccattt
      721 tttaaacttt ctcatgacct cttcataact tggtgcactc ttcaattctt cagaagatat
      781 atgtgttaat tcttgtatat gaggatacaa ctcctcatta cttactggtc ttattaaaga
      841 tttaaattta tcaatatttt ttgttttttc atcatacaca actgctccaa ttgcaattat
      901 atcagaatta aatactgctc tttttgattt agaatttggc atattcattt caaaatctat
      961 atatactctt ttcaatataa acaccctttc caattgttta tagtgattct ttttaactat
     1021 ttttattcaa aattttctaa gaaagaatgt ttctccccat ctttttgcca accaagtact
     1081 gaatcaagat ttacattttc tgccgcctta aatatagatt tttctacatt tttaaaattt
     1141 gattctaaac tctttattaa gtcttttatc tcatatcttt tattacctgt ttttttagca
     1201 gcaaccatac atgcacaatt taatggccat ataccactat tttgaataaa tcttataata
     1261 tgttcttctc ttatataata aagtggtctt ataattttta taccttcaaa attagtagaa
     1321 ttaagtttag gaagcattgt tttaaaattt ccagcacaca ataagttaag cattgttgtc
     1381 tctattacat cgtcataatg atgacctaat gcaagtttgt tgcaccctag ttcttctgcc
     1441 tttgaatata atgctcctct cctcattctt gcacacatat agcatggata atcttttgct
     1501 atttcatctg ctatctcaaa tatccttgaa tcaaataaat gtattggtat atttagatac
     1561 tcacaattat ctataagtaa ttgtcttata tttgcatgat atccagggtc catagcaata
     1621 aattcaacat caaaattaac ttgtccatgt ttttttaact cttgaaacat tttagccata
     1681 agaatactgt cttttccacc agaaatcgct acagctatct tatcaccttc ttcaaccaat
     1741 ttataatctc ttattgcctt cataaactta gaccataaat tttttctata tttttttatt
     1801 atactttttt ctatatctct tactggaagt ctttcctcaa aaggaactat aatttcacag
     1861 ccttttcctg atagttcact catctaatca cctcgtatat ttatgttaat aatattttac
     1921 aaaaaagtct accttcatcc acaaaatact ataattttta ctttaatttt actaaataat
     1981 ttatagttta caataacaca taaacttata ataacataaa taacctcttt cttttattat
     2041 cttttttatt tttctcattt aatattttat tattagtaac gatttttaaa tgattgaata
     2101 ttatacacta gatagagtta cctatctatt tacaacaagg aggttttcat cttggaaaat
     2161 aagactagag atatcaaaaa aaattgtggt ccatctttgg ctagaccata cattaataat
     2221 caaattttca cagaaatgta ctgcctttct gatgcattaa aatttggaac tatatttcca
     2281 gaactaaact tactagagtc tgaaatgtac aacaaagaat tatatgctaa gccaaaaaaa
     2341 tatagaggag gtagaagata atgaaagact catctagagc tgaacttatg agaaaagttc
     2401 aggaaacatc ttttgcatgt gttgatatga acttatattt agacaatcac cctgatgata
     2461 aaaatgctat caacacttat aattcattat gtaatcaatt tgcgcaagct agatatgctt
     2521 acgaaaataa atacggtcct ttaactaact ttgggtatgc tccaagtaga tgcccttggc
     2581 aatgggctga ccaaccttgg ccttgggaca gagaatttaa ttattagaca tcaagtattg
     2641 gaggattgaa aattcatgtg gatatatcag aaaactatac aacacccagt taatataaaa
     2701 acttgtgacc ctagaatggc taaatttctt ataactcaat ttggtgggcc aaatggagaa
     2761 cttgctgcat ctttaagata tttaagccaa agatatacaa tgcctactgg taatatgcgt
     2821 gcacttttaa cagatattgg tacagaagaa ctagctcacg ttgagcttat atgtactatg
     2881 gtttatcagt taacttctga tgcaagccca gaagagttaa aagctgcagg tcttggttca
     2941 aactatgctc aaaatggata tggaatttat ccaacagatt caaatggtgt tccatttgat
     3001 gtaagaccta tagcagttat gtcaaatccc gtaaccgatt tacatgagga tatggcagct
     3061 gaacaaaaag cacttgcaac ttattatcaa cttataaacc taacagatga cgttgatgtt
     3121 atagatgtat taaaattctt gggtcaaaga gaaataattc actatcaaag atttggtgaa
     3181 gctttaatgg atgcttacga gttagaagaa tctcaaaaaa tgttctaaaa tttttatttg
     3241 tataactatt attatttgat aatttataat ttactgttgt gttaaaattt ataaaaaata
     3301 taaatataat ttattttcta taaacagaga aaataaataa aaagcaggta tcaatcaaat
     3361 agtaacatgt attatactat acattactat caatattgta cctgctttca tattataagc
     3421 aataaagaaa aatctattta ttaggtttta taataaaaac aattataaaa ctcttcttgc
     3481 atttacatat ctacttgaat agtagctact atttatatta gatattgtta ctttttttga
     3541 tgaacctgaa gatgcatgta tcatatctcc atttcctatg tatattccaa catgacttac
     3601 agaaccatta tttgaacctt gtgtatcaaa gaatactaaa tctccacttc taaggtctcc
     3661 tctatttaca tatgttccat acttactttg gtctctagat actcttggta tactaactcc
     3721 tacagatttt ttcataacgt actgtgtgaa tccagaacag tcaaaactat ttggaccttc
     3781 tgctccccat acatatggtt ttccaagtag tgtctttgca aaacttatta cactatcagc
     3841 tccttgactt ggagaattag tcgaataaga tggaattgaa gtatcaacag aacctttttc
     3901 agcaaggtat tgacttgaca catatccagt ttgattgcca taacttactt ttgtccatcc
     3961 tccactttca gaaatacttc caacctcagt accataactt aatgtagcta ttttagaatg
     4021 acttgtacct ggtcctgttc taacgttcaa tgactttgct gttacaacct ttgtaccctt
     4081 tacaaggtca tttgaactgt tttcagcatt ttcatttgta gagtcattta cactagttcc
     4141 taaatattta cttgaaacat aacctactct accattagaa cttatttttg accatccatt
     4201 tgactcagat atgtatccga cttcagtccc atatcctagt gttgatattt tagaactact
     4261 tgtacttggt cctgttctaa aatttaatcc ttttgctgtt acaacttttg tcgacttaac
     4321 tgaagtatca ctactttcat tgctacttcc aaggctattt attgctacat aatttccatg
     4381 cacataacct gtgtttccat tatatttaac ttttacccaa gaaccttctt ttcctacgta
     4441 ctcaacttta tcacctttgt aaagtttccc cattgattca tggtttgtac ctggtccttt
     4501 tctaaaattt accgcattcc ctgttattgt tctgctttct atactcacac tctctacttc
     4561 agactcatta gcatatgcta aattccccat agtaggcatt attatcgcag ttgccattat
     4621 actggctgct attctctttt gattaaaatt cataactttc cactcatctc ctcttttcta
     4681 ctatctttat ttcacttcaa aatataaaag aaataattga cactattaaa atatcaatta
     4741 tttcttttat attaatatta ttacaaattt gtaactattt gtctatagta atatttgctt
     4801 accataaatt accatgtata aattttaatc tttaatagcc tgtaatttag taatatagcc
     4861 atcttttacc ttttttaaaa atggctatat tttttaactt ttttgtaact ttttaaaatc
     4921 cagttgagcc aaacccacct tttcctcttt cggagtcact taattctttt acttcttcta
     4981 tgttaatatc atagataggt tttactatca tttgagcaat tttcatatgc ttttcaacct
     5041 taaaatcatt tttaccatga tttattaata taattttaag ctctcctctg tatccttcgt
     5101 caatagttcc tggtgtattt aatacagtaa cagaatgctt taaggcaagc ccacttctag
     5161 gtcttacctg cgcttctgtc atagtaggaa gctctataca tatcccagtt ttgataagtt
     5221 tagtctctcc aggaggtatt acaacctctt ctatagaata caaatccata ccagcatcac
     5281 ctttgtgagc aaaattaggt attatagcat catcatttaa tttttttact tttaaattgt
     5341 acatcttata tctcctttca actttctata aatattataa cttaaatttc gacctcttga
     5401 agttttttat ttttttgtgg taatataatt attagtgttt taaaggagga ttttttatga
     5461 aacttgtact tgctgagaaa ccatctgtgg caaaaactat agcatcattt ttaggtgcaa
     5521 agaccagaca agatggttac tttgaaggaa atgattatat aattacttat gcagttggac
     5581 acttagtatc attatatgat atgaaggatt atgataaaga taaatactct ggctcttgga
     5641 aaatgaacaa tttcccattt gttcctgagg ataagtttaa atttaagatt gatagttcta
     5701 aaactaaaca attcaacact gtaaaaaagc ttttgaatag aaaagatgta gaatatgtaa
     5761 taaatgccac tgacaatgac cgagaaggag aacttatagc atttttaata tttttactgg
     5821 caaaaaataa aaaaccagta aaaagaatct tggtaaatga atggacacca gaagatatta
     5881 caagaggtat aaaaaaccta aaagatgaag atgaaatgag aaatcttcaa gctgctggat
     5941 atactaggct tattactgac tggttgattg gaataaattt cacttctgtt gctacattga
     6001 aatatggaaa tgggaaacta ttaaacatag gtagggtaat tcttccaaca gttaaattag
     6061 tctatgatag agatatggaa atactaaact ttgttcctaa aacctattat gaaatagaag
     6121 ggcattttaa agctgaagct ggtgaatata aaggtaaata tgttaagggt aaagaaagta
     6181 aatttgatac acttgaagat gcaaataaaa ttatagcttc aattacatct gaaactggta
     6241 aaatactaga taaaaaagtt actatgtcaa aggaatatgc accaaaactt tttagtttga
     6301 catcacttca aggatatata acttctaagt attccaattt tacatctgat aaggttttaa
     6361 atgtctgtca atctctctat gagggtaatg gtaagggtgg atacataact taccctagaa
     6421 ctgattccat tttcttagaa gaaagtttgg cttcaaaagc ttctcaaact ttagataaat
     6481 taaaggttgg tcttgaatat gaaaataaaa ttaaattttc taaaactaaa agagtttttg
     6541 attcttctaa agtagactcc catagtgcta taattccaac ttatataatt ccaaaaagtt
     6601 tatctaagga tgaacaatta gtttatgatg ccataaaaga tagatttgta gcaaacttta
     6661 tgcctcctgc tgaatatgag aatacagaaa ttaagactga agtagataat tgtactttct
     6721 taacaaaagg taaggtgctt aaatcaaaag gataccttga ggtatataat aaggaagaaa
     6781 aaaacgattt acttccactt gtaaataaag atgatgttgt agatgtgtta gaaataaaac
     6841 cattaactaa gcaaactact ccacctaaac catatactga ggacacactt ttaaaggcta
     6901 tgaaaaattg tggtaaaaat gtgcctgaag aagatacaac agtgctatct ggatattcga
     6961 ttggaacatc agccacacgt gctgatgtgt taaagaagat aagtcaagtg ggctatgtaa
     7021 agaaaaaagg taaatcttat tctataacag accttgggaa aaatttagtt gaaatttttc
     7081 ctgttaaaga cttatttgat gtagattata ctggaaaact tgaaaaaagt ttaagcgata
     7141 ttcaaaaagg aaagtataca agaaaagaat atcttactaa tatcattagt tttatatatc
     7201 aaaatgtcaa tattataaaa caagatgctt caaaaaatat aaacacagaa gcatatactt
     7261 tcgattcaaa aactaaaaaa tttactaaaa caaaagaaaa aaaacagaaa aaagatgtaa
     7321 ctaatactga aaaagaagtt gctatatcaa aggattctaa taacaaaaaa gaagttaaaa
     7381 atggtgatga aaatcaatct cttggcaaat gccccatttg tcaaggtgat gtactagaat
     7441 ttgacaaagg ctttgcttgt aaaaatcata aagaatgtaa atttgttatt tggaaaaatg
     7501 ataagtattt aagtctttat aaaaagaaag ttaacaaaac aatggtcaaa aatattttaa
     7561 aaaaaggtga aactaaagta aaatctttaa ctgctaaaaa cggtaataaa tttgatgcta
     7621 cattaaaata tgataaaaat ccagatactg gctatttcaa ttggaaaata gagtttgaca
     7681 actaaatatt cataagaaaa gaaatagcag tagtataata atctatatta tattactgct
     7741 atttcttttc ttatgaagtt ttaacttttt taatagtgac ttttacaatc acactctaac
     7801 ctaatttctt tactatttct gataacttta atgtatgttc atctaataaa gttagttcct
     7861 cttcagaaag attagataat ttttttacta gagattcttt gagaatagct tttactgcat
     7921 caaataattt aaatgcttta tctgttaatt caaccctaag aatccttcta tcttttgggt
     7981 caggaaattt attgactaaa ccatgttcca ctaatctgtc aataatcgga gtcatatttg
     8041 attttaaaat tcctaaatta gaagctattt gagaaattga tgatgaattt gatgtcataa
     8101 gataaagaat taccttagca tgtgatggag ccattggaca ttcttcaaaa taagctttca
     8161 ttatatcaga gttattttga aactttttta acaaatcgct ttctttaaat atattatttt
     8221 caagcacaaa taaaaaatct aaaaaagagt ccgctatttt aagtatattg ttgttatcca
     8281 tatttgtaac atcccttcaa tagataatat aataaaacat tgttgtttaa tatagtataa
     8341 attataaact attaataaat ttaaaacaag aaaaagttat tgactcataa taattataaa
     8401 tttataatca aattagagaa ttataaattg ccaataaagg gaagtttttt aattagtcta
     8461 aaactaaact taaattataa gacactttaa attctacaac aagctagtgt gcaatatata
     8521 ataggaggag atatgaaaag atatttaata gttttcttaa tgtgtagttc actgttttta
     8581 gttggatgtg gaaaaacaga agaatctgaa cctgagaaac caatagcagt ttctgttcaa
     8641 aaagcaatag gtggagaaat tgaaaacaca aattctttct caggaactac aaaggttaaa
     8701 gatgaaacag ctgttactgc acagactgtt ggcacagttc aagaagtgta tgtaaaactt
     8761 gggcaaaatg taagaaaagg agatgaatta ttgagtataa gcagtccaga gctagaaaat
     8821 agtgtaaagc aaagtaaagc ctcactagat ttagcaaaag caagttattc aagtgccact
     8881 ggtggttcat tagaggcaca agtaaatcag gcaaaaacag ctcttgacaa tgcaaagatt
     8941 caatatgagg agtctcaaag aaattatgac aataataaga ttctttatga acaggaagtc
     9001 attagtttag accaatttaa aaaaatagaa ttttctttag agcaaacaaa acaacagtta
     9061 gattctgctc aaagagcata cgatacagca acttcaaaaa gtatacctca agctaaagca
     9121 cttgctaaaa aacagcttga ccaagctcaa gtatcatata atttagcaat gagtaattta
     9181 gacaaattaa cattaacatc tccagtagat ggtacaataa cagctaaaaa ctttgattca
     9241 aaggaaatga taacacaatc acaaccagct tttattattt ctaatcctag tatactagaa
     9301 gttgatttaa atgtagcaga atcagatata ggtaaattta agaaagatgg aaatgtagat
     9361 gttataattg aagaccaaag gattttagga aaaatagatt atgtaccttc agtagttgac
     9421 cctcagacat ctttatatcc tgttaaagta cttgtaaata atacaaacaa caaatttaag
     9481 gcaggtatgt ctgctcaagt aaacttaagt gttgaaaaag aaaatggtgc tgttacagta
     9541 cctaaaaaag caatatttga agaaaatggt aaaaaatatg tatacatagc aacaaaagac
     9601 aatatagcaa agaaacactt ggttcaaact ggaattgtaa cagaggataa aatagaaatt
     9661 aaaagtggtg taagtgataa agatacagtg attattggag ggataagtct tatttctgac
     9721 ggaactaaga tatttcctgt agaaaaggag aagtagacta tgaatttaac acagacttcc
     9781 gttaaacgtc ctctcacaat cataatggtc tttttagtat taatagtttt tggtggtatt
     9841 ggatataaaa aaatgtcaat aaatctaatg cctgatatag aaataccagt tgtaatggtc
     9901 atgactactt ggacaggtgc tggtcctcaa gacgttgatg aacaagttag tcaaaaagtt
     9961 gatgaaagtt tatctgccgt ttcaaatgta aaatctacta tatcaagttc acaagaaagt
    10021 gtttctatgg tagttgctca gtttgaattt ggaactaatt tggatgaaat aatgaatgat
    10081 gttaggtcta aagttgatgc attacagacc agcctcccag atgatgcagc taaaccaaca
    10141 gttttaaagc tggatatgaa tgcacaggcc ataggtcaac ttgtcatatc tggaggaaat
    10201 gaaaatagtt cacaagcctt aagaaaatac gctgaagatg taattcagcc taaaatagaa
    10261 tccatagatg gtgttacttc agcagacctt aaaggtggaa aaaaagcaca ggttaatgta
    10321 atagctgacc cagctgttct ttcaaattat ggggttagtt tatccactat aaaaggtgtc
    10381 ttatcctcta gtaataaaac attcccttat ggttcaatta ctcaaggtga ggataaaata
    10441 gttttaagag cgattgataa attagaatcc ctagatgata taaaacagat tcaaattcct
    10501 gtaaaaggag gaaatacagt tagattagat aatctttgta gtgtagatta tggatatgca
    10561 gacaaagata gtatttatag atacaatgga aaagacagtt tggtaataga tatttctaaa
    10621 caacaagatg ccaacactgt aaaagttatg cagagtgcat acaaatacgt agatgaattg
    10681 aataaggaaa atcctcaatt taaaattaaa attgctaatg atacaagttc atacataagt
    10741 gaatcaataa acagtgtaat gagcaacttg cttataagtg ctgttatcgc atttattgta
    10801 atatttgctt tcctaaaaag tgtaagagct tcactagttg ttgcagttgc aataccaatt
    10861 tctataatag gtgctatagc aattctctac tttactggtg aaagtttaaa tcttattact
    10921 gcaagttcat tggtaatttc agttggtatg gttgttgata atgctacagt tgtaattgaa
    10981 aatatattta aatataggaa aagtggcaga ttgagtcttg atgattgtgc tattgaggga
    11041 acaagaactg taacaaatgc aatacttgca tcaacattga ccacaatagc aatattctta
    11101 cctatcatgt ttacagaagg tatagctaaa ataacttttg gggcattagg taaaacacta
    11161 atagcttcac tagcattctc ctttataggt gcaattacac tcgttcctag tgtatttgca
    11221 aaattaagca gaggaaaaaa tagtcaaaag atgcaagaaa aagatagtcc tatatttgat
    11281 aaggtaagtg aagcatataa aaaattatta agtgtatgtt tgagacatca acgcttagtt
    11341 gtaatatcaa gtttacttat ttttgtagct tccttatttg gaatgggatt tataggtatg
    11401 gactttatgc ctgcagcaga caagggtgaa ttatctattt ctatagaact tccaaaaggt
    11461 cttagcctag aatccaatga ttactatgta tctatgactg agaaaaaagt ctcagatatt
    11521 ccagaaatta aaactttaat aacaactttg acatcagatt caaataatag taaagcaagt
    11581 atagcaattg aattagaatc tcaaaaaaat agaaaaagaa ctactgatga aatagaaaaa
    11641 gagataattg atagagtggc tactgtacca gattgtaaaa taaatgtaag tcaaaacagt
    11701 agtgttatgg gtggagctgg aggagcagac ttcacattag aaatgaaagg tcctgattta
    11761 gatgtattaa aaatactggg aaaagaagct gaaaaagaat ttagctctat aaaaggattt
    11821 agaaatgttg aaacttctct tacagataca acacaagaag ctcaatttac tatagataaa
    11881 cataaggctc aagaatatgg tgtaaatacc gctgaaattg caggcatgtt acgtacagca
    11941 ataaatggtg actccgtaac cactgctact attgatgatt acaaagtgga tgtaaaccta
    12001 aaatttaaag cagatactat tgatagctta gaagatataa agcaactaaa agtaaactct
    12061 gcaactggaa aagaagttcc tcttggtgct ttttcagata taaaaatggc agatggtcta
    12121 caatctatat ctaggactga tggagatttt tctgtaaata taactgctac acttgacaat
    12181 ttagatgctg gtactgctac aaaactagca ctagaaaaaa cgaataaaat ggtaattcct
    12241 aaagattact ctataggtgt tggtggtgaa acagaaatga tgaatgaatc tatggcaagt
    12301 cttggatttt caatggtcat agctatagca ttagtttata tggtaatggt agctcagttt
    12361 gaatcattta gtaaaccatt tataatcatg ttctgtgttc catttgcatt cgttggagtt
    12421 gtgctttcgc ttctagttag tggtcaaagt ttaagtactg tcggtatgct aggtataatc
    12481 ttacttatcg gtattgttgt taataatggt atagttctta ttgactacat agagcaactt
    12541 agaaaaacaa atcgtaacaa tgatttaatt ggtttggttt caattggttg tgcagcaaga
    12601 ttaagaccag tcttaatgac tacagcaaca acaattttag gtatgttgcc aacagcacta
    12661 tctcttggtg aaggtggaga aactatgcaa ccactagcca tagttataat gggtggtcta
    12721 actgtatcta cacttataac tttggtgctt atacctacta tatacttaat atttgataaa
    12781 tgggaaaata agttttcaga caaaatgcct aaaatatttg gtaagataac agataaaata
    12841 ggaagtataa aacgtaaaat aataaagaaa ccaaaattga aagctaaagt ttaaatagaa
    12901 tggagggtga ttgtattgaa taaaaaaatg agcaaaatag ttgcaattgg ctgtggtgtt
    12961 ggtataataa ctgccaattt aatcccagtt tacgcagata ataatacttc ttctataaac
    13021 aaagttgata attctatttt aagtgactca aataaaagaa catctgaaaa ggaaattttg
    13081 actgctagag aagctgttag tgctgcactt aaaaatagtg aaaaattaaa aatgaaatca
    13141 gaagaaataa aaatgttaaa agaaaagtta gaagtacaag atgagtttga tagttttact
    13201 ggaagtgaca actcgtttcc atacgaccaa attggacttc ttaaaaatca aagtgaacag
    13261 gctaaaggct ttatggagga ccaaatagca aatgatatta ctaataaatt taacgattta
    13321 gtttcaagag aaaatgagct agacaagata aaaaacaatc ttgaaataaa aactaaagaa
    13381 attaaagata tgaaattaaa aaaagattta gggttagtaa catctttaga aactgaatca
    13441 gctgaacttg aacttcaaac actacaaaat actcaaaaag ctaaacttca agaactaaaa
    13501 aacaatcaag actactttaa gttgttgact aatatagatt taaataatta tcaattggat
    13561 aaagaatata gatttgaatc ttttagagta ggtggctcag ttgattcata tatggaaggt
    13621 aaggttaatg aatatttaaa atacgaccaa ttgattttag aacgtacaga agaaagcttt
    13681 aatgataaag atgagaacaa agcagattta ccagatagac ctaatcttac aagaccagtt
    13741 gctcctacaa aaccagagca aggtaaccta actgatgatg aatataaggt tcttacggat
    13801 aagtatgaaa aagactacaa gagatatgaa aaagaagttg aagcatacaa tcttgaaaga
    13861 cagacttatg ctgctggcct tactacttat gcaaattatc ttcaacaaaa atttaatacc
    13921 gaaaatggtc tagtaacatt agaagacagt aaaaaagctc ttaaaaaagg gttaattgat
    13981 agctatgctc aattacttgc tttagaagat acaattcaga ttactaaaaa acaacttgat
    14041 ttatcagaaa aacaattaaa aaatactaaa ttaagatatg atttaggatt aattactcta
    14101 actgattata aaaaacaagt ggtaagtaat gaagatgcta aaaatagcta tgacactctt
    14161 attgtgaatt ataactcact aaaaaatggt atagaaaaac cttggatact taatactgga
    14221 aaataattct ggtaaataga aagattactt taacatacgt ttgattaaaa aatagttatg
    14281 cacactcgtg gctttagtca tggaagattc agaaggcaaa taatagaaat agattagtaa
    14341 aaatatattt gcttataaaa ctgtataatt ttatagaggc tatctcaaaa tagagataag
    14401 cctctttttt attgtttaaa ttataaaaat atatttaatt ccatacaaaa agagtgcctt
    14461 atgaactaaa aagttcattt tgagacaccc tcaaaagctt aactctaaat ttaattattt
    14521 tttatcaaat gtctcatcta ttttacatct caccatgtta acaaattcag aaatattata
    14581 tcctctaagt ttgcatccac atttaggaca ataatccaat ttcctagata ccaaagccct
    14641 acaactaggt tctggacatc ttatttttag actctcatct gctactttaa atccacatcg
    14701 tgggcatata ctacctggac gtgatgatac ttcctttcca catttaggac actttgtcaa
    14761 ctttatcccc tccagtaaac tcaatttaat tgatagttga aatttctctc tacaataata
    14821 tatgcaatat aattaaatac gtttcactta caactatata agttaaattt ttataataaa
    14881 aatcccctat attcaattta caaatactta atttgataca ttgaacaata ggggatttta
    14941 tttttattta gttacatatt ttgcagtcta ttattatcta ggatttttta tagatgcttg
    15001 agctgctgca agtctagcaa ttggcactct aaatggtgaa caagatacat agttaagtcc
    15061 tacactataa cagaactcta ctgaagatgg ttctcctcca tgctctccac atattcctag
    15121 ttttaattct ggtttaactt ctctacctaa tttagctccc atcttaacta acttaccaac
    15181 accattttgg tctagtactt ggaatgggtc tttttctaat atttcttcat ctacatattg
    15241 acctaagaat ttaccagcat catctcttga atatccaaat gccatttgag ttaagtcatt
    15301 agtaccaaaa ctgaagaagt ctgcttcttg tgctatttca tcagcagtta aacaagctct
    15361 aggtatttct atcatagtac ctacagtata aggaacttct ataccttctt cttttattat
    15421 agcgtcaaca gtttctacta tcatttttct tattattttt aactcattta cttctccaac
    15481 taatggaacc attatctctg gagaaacttt aacacctgct ttgattgctt ctatagcacc
    15541 ttgtattata gctttagatt gcattacaca tatttcagga taagttattg caagtctaca
    15601 acctctgtgt ccaagcattg ggttaaactc atctaaatca actattctct tcttaatatc
    15661 acttactttt attcccatag attttgaaag ttcttctata gtctcatcat catgaggcaa
    15721 aaattcatgt agtggtgggt ctagtaatct tatattaact ggttttccat ccataacttt
    15781 aaataactct acaaaatctt ctctttgcat tggtaatatt ttttcaagag ctttttctct
    15841 ttgttctact gtattagata atatcatttc tctaactgct ggcaatctag cttcatcaaa
    15901 gaacatatgc tcagttctac atagacctat accttctgca ccaaactcta tagctgcacg
    15961 agcatctcta ggattatctg cattagttct aaccatcata catttgtgtt tgtctaccca
    16021 gttcattaat ttttcaaaat ttccagttaa agatacttct gttttcttaa catctcctag
    16081 gtatacattt cctgtagaac catctataga aatgtattct ccttctttta taactacatc
    16141 atcaagtgct cttatttctt tattaaactc atcaactttt atttctccac atccagcaac
    16201 acaacattta cccatacctc tagcaacaac tgctgcatgt gatgtcattc cacctctagc
    16261 tgtaaggatt ccttctgctt taaccattcc ttctatatct tctggagatg tctctagtct
    16321 aactaaaact actttttctc ctttttcatt tgcttttact acatcatcag cattgaaata
    16381 aactttacca cttgctgcac ctggtgatgc tggtaaacct tttgctataa ccttagcttc
    16441 ttttaatgcc ttatcttcaa attttggatg taataattgg tctaattgat taggctcaat
    16501 tctcatgata gcttcttttt catcaattat tccagcttca actaaatcaa ccgctacatt
    16561 tattgctgct gctgctgttc tcttaccatt tctagtttgt agtatatata atctttcatt
    16621 ttctatagta aactctatat cttgcatatc tttataatga ccttcaagta tatgagtaat
    16681 ttttacaaat tcatcgaata cagctggcat tacatctgca agagttgata tattttgagg
    16741 agttcttata cctgcaacaa cgtcttcacc ttgtgcattc attaaaaatt caccaaataa
    16801 tttattttct cctgtagctg gatttctagt aaatgcaaca cctgtaccac tagtttctcc
    16861 catattacca aataccattg attgaatatt tactgctgtt cctaagttat gagctatatc
    16921 attcaattta cggtatacga ttgcacgagg attattccat gatctaaata cagcctctat
    16981 tgcaagcatt aattgctctt ttgggtcttg tgggaaatca ttttttattt cttttttata
    17041 aattgcttta aattctttta ctatttcttt taaatcatca gttgtaagtt ctgtatcaac
    17101 tgtgtatccc tttgcttctt ttactctgtc taatacattt tcaaatttat attttggaac
    17161 ttccatagca acatcagaaa acatttgtat gaatcttctg taactatcat atgcaaatct
    17221 ttcattttgt gtagcctctg ctaagccaac aacactattg tcattaagac ctaggttaag
    17281 aattgtatcc atcattcctg gcatagagaa tactgcacca gaacgaactg atactaataa
    17341 tggattctta ttgcatccta atgttttatt taagtctttt tctaaagtag ctaaatgctc
    17401 ttctatttct tttattatct ctgctcttat actttcattg ttaacataat aatcattaca
    17461 tgcttctgtt gttatagtga atccaggagg gacaggtaag cctatcttag tcatttctgc
    17521 taaattggca cctttacctc ctagtaaaga tttcatgtct ttgcttcctt caccaaaact
    17581 ataaacatac ttagtttcca ttgttaaatc ccccattaat ctttttattt taatttatat
    17641 catttttatt aatagacatt attaaatttc tatttctttg tatatcttta gaccattttc
    17701 tttcattatg tctaaaatta tacctgctgt ttcttctatt gccttatttg aaacgtttat
    17761 tactgggcat ccaattcttt tcattatctc ttctgaataa tctaactcct gaagtattct
    17821 ttctaaatta gcatagtttg ctttactgga caatcctaaa gcttttaatc tttgggttct
    17881 tatctcattc agcttttcag gtgaattagt aagacctatt atctttttag tatctatctc
    17941 aaacacttct ttaggaattg gtatttctgg aactaatgga acattagcta cttttatatt
    18001 tttattagct aaatacatac tcaaaggagt tttagatgtt cttgatattc ccactaaaat
    18061 tatgtctgct tgaagaacac ctctagggtc ttttccatca tcatatttca ctgcaaattc
    18121 tatagcttca actctcttaa agtaagattc atctaatttt ctaattatac cagcttctct
    18181 tttaggtttt cttcctgttc tcttgcttat ttctcttagt atatctgtca ttaaatctat
    18241 acaactaaga ccttctcttt cgcaatatct ttcaacaata ctgcataatt cttcatcaac
    18301 taatgtatat acaattaaag cgttttcagc ttttgcattc tccaaagttt caagtagtac
    18361 tcctttatta atcatgtatg gaaatctttt tatttcataa gtttcattca cataaaactg
    18421 agacatacat gcctttgcaa cttgttgtgc agtctcccct actgaatctg atactgcata
    18481 tataattagg tttttcacca gcatacctcc ttaattgtca actatttcta agaatagctt
    18541 agttatgttg gtttttgata ttctcccaac aactctcata tgatttttgt cttctccctt
    18601 atattcaaca atcggaatcg aatctacttc atgttctatt atttttcttg aagctaataa
    18661 aacacttgct cctttagtta aagtgactac atttggagtt cttgtcatta tcatacctat
    18721 tggcatttta tttatatcag aacctcctat agttgctttt agcaagtctt ttctggaaac
    18781 aactccacat aactcttcat tctcatctat aataactata ctccctacat cagataaaaa
    18841 catagtgact attacatcat atatactttc atccttctta gctagtacag gcatgctcat
    18901 tatatcctct actgtttttt cttttacact tttaccaatt aaatttattt cactaactcc
    18961 tgaataaaaa tatccaactt ttggtcttgc gtctaatatt cctgtcattg ttaatatagc
    19021 taaatcagat ctaagtgtag cacgagtcac attcaagctg gaggcaatac tttcactcgt
    19081 tattggttca ttttccttaa ctatatctat aatttttagt tgtctttcat taagctgaat
    19141 aataatcacc ttctttcatt gtgctatact ttttatcttt tcgcatctta tatagtatat
    19201 tatataacta tatataccat tctgtaaaca gattttttat tgaatacgat atatttttta
    19261 ctaagttctc tcgcttaaag atatttatta tatattaatg ggggatattt ctttaattaa
    19321 tagaactata ttagtattac agttaatatt ataatacata attggtcatt ttaattcatt
    19381 ttatattaaa atttttaata atattatttc aatgtgaata taaaattgca gaactaattc
    19441 aaaataattt ttacgactac aaataaccta taatatactt ttaaagaaag tatcctaaca
    19501 agaatatata aaaatttaaa agggttgatt taaaaataac catcaaccct ttttactaat
    19561 atctatttat attaccatca aatcttaccc aaatattttt tcttgcaatt gtcttaaatg
    19621 atataaagta gtatcttttt ctatctctac catatcgtaa gttataactt cacctttttt
    19681 aatatctttt acaactttag tctttttact tatcaaacca ataggtacag cattcatagc
    19741 ctttgctaca tcgtattttt ctatagtacc atagcatgta tatgctccat agccatccat
    19801 aaactcacct tttttcaaat ctttcttagc tatagtcata acttctgcta ctagtccagc
    19861 tttaggtact attgtagcct tatggtctaa aactgccata gctgctgata aaggagtctc
    19921 taaactacat aaatgatatg gtctatataa tatgtaatta ggtccatcac ccatacttaa
    19981 atattttagt tcatcattaa ctgcttttaa tttatgggca actactacga aaactccagg
    20041 tgctatacca tttacaaaat caactacacc ataattatct aatactccac cttcgctttt
    20101 taatgcatat ttcttaggta cttcattaac tgtagcttca ataccatgac cacctcttac
    20161 atcaggcaca aatccagttg cattacacat taaagccatt tctaccattg ttttagttcc
    20221 ttctttaaaa gaagctatca tatgaggtga agctcctttt ctttctgctt cttctctaac
    20281 tgtatcagga ttacaatcta aatctaattt attattttta cctttaccta tcactctaac
    20341 ttcaaacccc attgcatctg caaaatcaaa taattccatt actgcacctg gctcatctcc
    20401 tgcagaacct gtaaatatta ctccagcatc atctgctaat tttttaagta ttggtccaac
    20461 aacacaatca gtttcagcat ttaacataac tatatgtttt ttatttaaaa tagagtttaa
    20521 tgctatctct gcaccaattg caataccccc agttgcatct ataactacat ctattgactt
    20581 agttttacaa gccacaagaa agtcattaca tgtcaatact tttccttctt gtaattttat
    20641 ttctgcatcc ttttcatttg ttacattatc tataatttct tcatctattc ctgcatcagt
    20701 aaaagcttta tgagccaact ccaaatctat atctacaaca atagaagctt caataccttt
    20761 catatttgcc atttggctta ccattccatt tcccatatgc ccagctccta taagagctac
    20821 ttttattttt ttaccttcct cttccaattt tgcaagtttt ctgtttaaat ctaacatacc
    20881 aattcctcct gttataataa ttttattttc aaattaagaa atagttatta aatctccaat
    20941 agttatatct ggatttccta tttccacatg tatttctcct ggtaaattta cttcacattt
    21001 tccatcaaat ttgattgtac aatgcccaat ttctctaaga gtcttctgag caatatcacc
    21061 aactgaaact atacaataat ctctattccc tatagtaagt gtatctccta tttcaacatc
    21121 actttttaat gttgctattg aatgaattac agatatatcc tttaagtcgt catcattaat
    21181 ggtttcatca tatataataa gacaattgct ttcatccatt aactctttaa ctaaactgcc
    21241 tatcccactt atcttaactt catatttcat gacattcctc ctgataattt atatttatat
    21301 tgagttaatt aaaagaaaca tttatttttt cttttataaa ttttatagct tgtacaatac
    21361 tctcttcatc tttaaatttt tgctctgcac tatatatatc taatccttct atttcttcaa
    21421 tctctttaaa tttagccatt actgtacgcc cttttaaaat ttctccaaac tttataaacc
    21481 catctgaatc tgaagatata acggctatac ttcctttaga aaaatagctt ttctttttgc
    21541 ctgtagacac tatatcaccc ttttttttag ctctatttaa agttttaaaa tacaatttat
    21601 tctgcttcat tataaatata gtatttaaaa tataagcaac aaataaaatt actaataatt
    21661 tcatatttat acaccttcca aaaatcacat tatttttaaa tctgtataac aactataaat
    21721 gctgtacaat agttatataa ggtttttaag taacaccttt ttaaattcta aaaaagttct
    21781 acatttactt atcttattcg ttaattttac atcttggcct atctttaaaa cttcttcata
    21841 aatttttact aataagtctg agtctatctt attctcacat ggaaacattg tcataagaat
    21901 tatttttata cttctatttg catattctat tggttcttct aaaattccaa ctgccattaa
    21961 aaacttatct gatttttcat ttatagaatg aggaaataat aaacctttat caaatactgt
    22021 gggagatttt tcttctctat tgactatatt ttttctaaac ttctcatcaa tacatcccaa
    22081 atccataaga tggtccatca ttttttctaa tgaggcaagt atagtctttt catttaatat
    22141 cataaattta tcttcatcta acaaattatt tattagtaaa tttgtagaat tagtagtgtc
    22201 aatattccct tctcttaggc acaatactgt ttttaattgt tctcttagtt tgttttcatc
    22261 aaatattgta tttattttta atactattgt atttatattt gatgtgtcta aatcaatagt
    22321 tgtaaaaact atatcgtatt catctaagtt aatatttctc aaattaaaac ttgaaagagt
    22381 atcaatttta ggattatttc ctatcagttt ttctactctt attttcaata gttttgctgt
    22441 tcctagacct gtactacata taattgctat tttttttatt gaatagaatc tactgctatt
    22501 tctttctaaa tatccactga aatggattgc tatatatcca atctcatctt ctgaaacttc
    22561 taagttatac tttttctcta ttacttttcc tgctatttgt gctaatttaa atggaagtat
    22621 ataattttgc ttaatctctt ctaacaaaac attttttaac tttatattaa ataatagcct
    22681 gtttaaagta aaactaagat gatatcctag acctactctt aattcatcct catttataaa
    22741 aatacccata tcgacatata tttgattaaa tatattttcc atcaactcat caatgttttt
    22801 actaattcta ctactattta aagcacattc tgatgctggt gcatttccac taactaacgg
    22861 tatagttaaa taaataattt cttcatcatt taaagtacat tcaaacattc ctgatattat
    22921 atgttttatt tccctagcaa taaagtattc atcatatgat ttaagtaaat caaactttac
    22981 atcataatct tttactttct tttcttcttt tactctatag actaaaacta ctatgtagtt
    23041 aataacttct cttaacattt catctgtaac ataaaaatta ttttttttaa ataaagtcaa
    23101 tagtttttct tctaaattta tgatttcttc ttttttgatt cctttaaaat actttgaatt
    23161 actgaaatct ttccttgagt ttttatacaa ctgatttaat ataaacagtc taatatcaag
    23221 ttcatttcca tttaatttca tacctgtatt ctgtttttta atcaagttta aattatatga
    23281 agccaaaact ttttctacat actggaaatc atttactaaa gtagttcttc ctacacacat
    23341 ctcatcagct aaactatcaa gtgtaataaa tccatctagt tctatcaagt aatttagtat
    23401 aaactctact ctatcttctt ttgaatttaa tgaaaataca ttttttctat tcatatcaat
    23461 aatcttattg agtatctgct catcttttat ttctagttta tagccttgac cctttatata
    23521 tttgattact gctatatcat tacctaaatc tttattcaat tgttttatat acgtccttac
    23581 acttctagtt gtgacattca attcatttgc tattgagtta atactcacaa actctttttt
    23641 actacacata atcaatatct taaatatctt gcggtcaata gaaggaattt tctcagttac
    23701 actcattttg tatctcctcc ttgatttcat tctagtacaa atttgaaacc aaattaatac
    23761 ataaaatttc actttaataa aggaaaattt agtattaatc taactcgata attacagcta
    23821 attaatagta taaaagctag tctttcatta acaaaaaatc ttgactagct taaatttata
    23881 tttacaattt tattaattaa ttccaagcct tatattgaca ttaaatatta tctcaatgca
    23941 aatactgcaa ctccatatgc aataataaca gctattggac cagttattaa acgagagtaa
    24001 aataaagctg gaacaccata ctctacagtt tctggttctg cttctcccaa acttaatcct
    24061 actggtacaa aatcacatcc agcttgccca tttattgcaa ataaagctgg aagtgctaaa
    24121 tatgctggta ttgcaccaag accaatttgt gtacctacaa gagtccctac tacttgagct
    24181 ataactgcac caggtcctag tattggtgat aagaatggaa gtgtgcaaat tactgatata
    24241 atcaataatc caccaatatt acctgcaagt ggagatataa ctcttgcaat ccagtctccc
    24301 agtccagatg ctaaaataat tcccataagc atacttacaa atgccataaa tggtattacg
    24361 ttattcatta ccatttggat agtatctcga cctgcatcat aaaattttga tactacttgc
    24421 cctgcccctt gaccaataga aagtattatg ttctttttag catattcacc ttgtgctttt
    24481 gacttaacct catcataatt atcaggcttt ctaatagaac tcttattttc gcttttattt
    24541 tcttgagatt tttgtggcat atcttcccca tctacaacac ttatacagtt aggattaaca
    24601 tctgatacat aatattcttc tgttataaat tttgctaaag gacctgtttt tccaactgga
    24661 tttacattta ttgtaggtat ttttttcttt ggataaactc cacatctagc agttccacca
    24721 caatcaataa taactactgc tacttcttca tcagaaactc cagttttaaa tccatctaca
    24781 atttcacaac ccagtagttc ttttattttt aatgttactt catggatacc gccagcagtc
    24841 attgatagta tcttatttct attcccttct ttttttatat acaaaggacc tccccagcct
    24901 gaagttccct tttctatttt taatatttta ttctcctcca ttttatctac ctcctaagcc
    24961 ttatttgttg ctctattttt tcttgttaga aaagcataca ttttttctgt tactatacct
    25021 cttataaaaa ttacaattag accaactata aaataccaca atgccaatgt agtagtttct
    25081 ttacctaaag tagtcaaacc tgcagatatt cccaaccata caaaaagttc tcctgcattt
    25141 gcatgtggaa aaagacctgt aattggatgt acaaatgaaa ctgctgcatc ataaaatcct
    25201 gctttgtgct cttcttttaa aaatctacca aatgtatagc acataggatt tcccaagaaa
    25261 aatactgata ctaatggaag tagaatatat cttgtaaaag agttctttcc tagcttagaa
    25321 gcccaattat taactctttc ctcaccaatt atcttaatca aagagttaat agctaccatt
    25381 aatacaatta gcgttggaag agtacctgaa acaagaccta taaattgttc tccccctttc
    25441 tgaaacaaac cgataaacca ctctgcacct ttacttagac ctgtaataac gttttccata
    25501 aaattataac ctccctataa tatctttaaa tttttaattt tactgcttac cataaatttt
    25561 acaaattaca tcttttttct gtactat
//
LOCUS       DAYATN010000044        25469 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.044, whole genome shotgun sequence.
ACCESSION   DAYATN010000044 DAYATN010000000
VERSION     DAYATN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.044"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            131..1096
                     /locus_tag="ACGI8A_001748"
     CDS             131..1096
                     /locus_tag="ACGI8A_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFH0179746.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQSYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(1294..3405)
                     /locus_tag="ACGI8A_001749"
     CDS             complement(1294..3405)
                     /locus_tag="ACGI8A_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HFH0179747.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(3707..4414)
                     /locus_tag="ACGI8A_001750"
     CDS             complement(3707..4414)
                     /locus_tag="ACGI8A_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFH0179748.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            complement(4613..6526)
                     /locus_tag="ACGI8A_001751"
     CDS             complement(4613..6526)
                     /locus_tag="ACGI8A_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFH0179749.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(6610..7701)
                     /locus_tag="ACGI8A_001752"
     CDS             complement(6610..7701)
                     /locus_tag="ACGI8A_001752"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0179750.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(7707..9074)
                     /locus_tag="ACGI8A_001753"
     CDS             complement(7707..9074)
                     /locus_tag="ACGI8A_001753"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HFH0179751.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            complement(9028..9780)
                     /locus_tag="ACGI8A_001754"
     CDS             complement(9028..9780)
                     /locus_tag="ACGI8A_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0179752.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(9828..10736)
                     /locus_tag="ACGI8A_001755"
     CDS             complement(9828..10736)
                     /locus_tag="ACGI8A_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0179753.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(10758..11507)
                     /locus_tag="ACGI8A_001756"
     CDS             complement(10758..11507)
                     /locus_tag="ACGI8A_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0179754.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(11536..12711)
                     /locus_tag="ACGI8A_001757"
     CDS             complement(11536..12711)
                     /locus_tag="ACGI8A_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFH0179755.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(12726..13565)
                     /locus_tag="ACGI8A_001758"
     CDS             complement(12726..13565)
                     /locus_tag="ACGI8A_001758"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0179756.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(13568..14725)
                     /locus_tag="ACGI8A_001759"
     CDS             complement(13568..14725)
                     /locus_tag="ACGI8A_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HFH0179757.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(14741..15463)
                     /locus_tag="ACGI8A_001760"
     CDS             complement(14741..15463)
                     /locus_tag="ACGI8A_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0179758.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(15475..16548)
                     /locus_tag="ACGI8A_001761"
     CDS             complement(15475..16548)
                     /locus_tag="ACGI8A_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HFH0179759.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(16570..18276)
                     /locus_tag="ACGI8A_001762"
     CDS             complement(16570..18276)
                     /locus_tag="ACGI8A_001762"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFH0179760.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(18322..19878)
                     /gene="murJ"
                     /locus_tag="ACGI8A_001763"
     CDS             complement(18322..19878)
                     /gene="murJ"
                     /locus_tag="ACGI8A_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFH0179761.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            complement(19993..21048)
                     /locus_tag="ACGI8A_001764"
     CDS             complement(19993..21048)
                     /locus_tag="ACGI8A_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HFH0179762.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(21143..21829)
                     /locus_tag="ACGI8A_001765"
     CDS             complement(21143..21829)
                     /locus_tag="ACGI8A_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFH0179763.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(22049..24073)
                     /locus_tag="ACGI8A_001766"
     CDS             complement(22049..24073)
                     /locus_tag="ACGI8A_001766"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0179764.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(24327..>25469)
                     /locus_tag="ACGI8A_001767"
     CDS             complement(24327..>25469)
                     /locus_tag="ACGI8A_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HFH0179765.1"
                     /translation="ISNNAVDSKLMNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQ
                     LRHAVWHGVTLADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILF
                     GFFLNYLGNPDLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTF
                     IIVGYYILAKPYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCT
                     MGCVLLKENIGEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGI
                     LLSIFYFICDIKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTL
                     VEWTTYELITPWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
BASE COUNT         7947 a         4635 c         2760 g        10127 t
ORIGIN      
        1 tttcttataa attatatcat tgtctttttt ttgtaactga aacaagtatt gaatatatct
       61 caatacttta atataattaa gtgtatgcta ttaaaaaata tacaggaaaa agaagaaaga
      121 aggggtactt ttgtcaaaat taaagaaatt tgttatacta ctggctttct tggtagtaat
      181 attccctata agtgtctatg gatattttta ttataaatta tctgctattc atgactcgag
      241 cataagtagt gatttattgg ataacaatga ccataaaaat gaagatggga taattaacat
      301 actacttatg ggaacagatg ctagaccaaa tgaagattct tcaaggtcag atgctatgat
      361 gatacttact attgataaca aacacaatga tatcaaactt acatcattag ctcgtgatag
      421 ctatgtagac atccctggtc atggtaaaca aaaacttact catgcttatg cttatggaca
      481 agctgattta ttaatacaaa caattgaaga gaattttaat attgatattc aaagttatgc
      541 ttgtgttaat ttcgaatcat ttatgtatat aattgatgct attggtggcg ttgaggttac
      601 tgttgaaaaa ggagaaatac gagaactaaa taaatttatt cctgaaactt ataaatggaa
      661 taaaagtgat gataaaggta gcatacaata cattagaaac tctggaaagc agactctaaa
      721 tggatatcag gcactatctt ttgcacgtat cagacacaac gatacagcct ttgctagaga
      781 tggcagacag agacagatta tacaagctat aatcaagaaa acggaaactt tacctgttac
      841 taaatatcca ggtttattag atgcagttct tccatatgtt aaaactaaca tgaagcctaa
      901 tgcaatactt tctttaggag ctcaagtttt aaaaatgggc gacttaaata ttaagcaatt
      961 tgaatttcct attgatgatg aaattcatag tacaggtggt atatatggaa aagctggatg
     1021 ggttcttaga tttgacccag atactttaga catattacac gattttatat ttaatgacat
     1081 agagtttaaa caataaaaaa ttcaacataa agtttattaa aaagtataag attaattact
     1141 aaaaaagaac tatatcgcat aattattttc aagttgctgt gtacacttga ttacaaaatg
     1201 catatagttc ttttttattt tctaatttat catgataatt ttcattttaa tctacattta
     1261 taaaatactc tgtattttaa attaactttt ttattactta actaagttta agaaagtgtt
     1321 taatgtgttt tgatttatac catctccagc ttcgtatact agagtagttt tcttaggctc
     1381 taaagcagtt ttctgagcag atgttacact atttccaagc ataactactg gtgattttaa
     1441 tttagctgct aaaggacctg atgttaatgc atctaccaat tgattacttt tacttacaaa
     1501 cattgatgaa tattcagcat ttggatagaa tttttgtatt acttttccat ttgtttcttc
     1561 tctattttct ccagctattc tatttttaga tacatcattt gtaatgacct tatccagctt
     1621 atttattact gaatcagata tacttagatt accacctata aaataagcat cttttacttt
     1681 caaatctgat atccaattgt aaacttcatc acttacatta tcttttgata ctaatactat
     1741 tggttgcttt tcttctcctg cttttgaagc tatagaaaga gaatctgctt cacctgtacc
     1801 acttgttata tatagttttt ctactggatt agtttttact aattcttttg ctatcattaa
     1861 agatgtacta tatctatctg aaccaccaac tctatttatt gatacatttg acaatgtatc
     1921 ttttatctca ctctcaactt tatctccaat agagttattt cctccaatta aaataacttt
     1981 acttggatta agtcttttta actcattctt agtggatgta ggtatattat ccttatttac
     2041 caatagtata ggagcatcat ttgatgtagc aagtggtgtt gatgttatac catcaactat
     2101 agagtaccca tttactaata ctactgtatc tgaatttgta gcccaacctt ttttacttat
     2161 tgctactgct gtgtcatatc tttcgtcgcc tcttaatttc tccactttaa cattagatgg
     2221 tggagtaact ggttcttcac tactagaata taaactcttt aaatcctttt gtatagaagt
     2281 actatttgct atatctttag ctgagaaata catactacct tttatttcac tatactttct
     2341 atttaatgtt acttgttgta ctatttcttt tgctatattt tgtccaccat aactcgattg
     2401 accttgctta tatatacctt gtcctatgta taagtctaca tttgttcctt ttacttcatt
     2461 agcccaccat gccactaatt tagaataatc tgctgcttta agaccaattg gccagtaaag
     2521 ttgtggaact acatagtcaa tcaatccttg ttttatccat gctcttgtat ctgcatatgt
     2581 acctacataa ctttcattac ctgatgtatc tgaacctgtt gggtcactac tcttatttct
     2641 ccatatacct gctgggctta caccaaatac aacattaggc tttatactct ttatagacgc
     2701 ttttacatct ctaagtagag tatttacatt ttctcttctc caatcatcct tattttgtcc
     2761 ttttccatat ttttggtatg ttgcagtatc attgaaactt actcctggat agaagtaatc
     2821 atcaaaatga actccatcta tatcgtaatt ttgaactact tctgctatac tatccactat
     2881 gtactttcta acttctggta aacctgggtc gtagtaatac ttatttccat gtttaactac
     2941 ccagcttgga tttttctttg ctggatggtt tgctggcaat ttatctatgc tctcatctgc
     3001 catagttatt ctatatggat taaaccatgc atgaaattcc attcctcttt tatgtgcttc
     3061 ttctattaaa aatggtaatg ggtcatatcc agggtcttta ccttgtgtac cggtaagata
     3121 ttcagaccat gggtttatat ttgatttata taaagcatct gattttggtc ttacttgaac
     3181 cactgctgta tttattccta cactttttaa tttgtccaat aaatctgtgt attccttttt
     3241 ttgcttggct tcattattct tagtcttagg ccagtctaaa ttataaactg tagatatcca
     3301 agcagctctc atttccttat catttgatga atcagcaaat gaacttacac tacacatagt
     3361 aagtgtcatt atcaatgata atactaatat acttattttt ttcatttcat cccccttaat
     3421 tttttaaatt tattttttct acaatatact tcttattaag tatatctaaa attcatatta
     3481 tctgtcaaat attgaataat atagttaaaa tttatatttg atatttgatt ataatatgaa
     3541 ataatttatt ataaaaaaaa cagcaaagaa atttatcttc tttgctgttt ttataacgtt
     3601 catcactatt ttaatattac taaactctta aatctctatg taaaacatta aatgtaatta
     3661 tatatttaat gttaatattc aatcctatgt aaatcaatat ctattattat tgtaaaacac
     3721 gtgtagcagt tataagtctt cttgcataat tagttcctgg agccatgcta gtaactttta
     3781 ctacatctcc tgtttgtggt gaatgtatga atttagaatc tcctacatac aatccaacgt
     3841 gatttattga accacctgaa ccaaagaaaa ctaaatctcc tggttttaat tctgctcttg
     3901 atactttctt tcctactgtt gcttgtgatc ttgatactct tggtaaatta actcctgctc
     3961 catttttata gacataagaa gtgaacccag aacaatcaaa agaatttggt ccagttgctc
     4021 cccatttata tggcttacct tgttgcttaa atgctaagtt taaaacattt tgtacctttg
     4081 catctacatt tccaccttga atagttaaat aatctccatt tacccatcct gtaacattgt
     4141 caacactttt tattttaatc catccatttg aatgtaattc taaaacttca actttgtctc
     4201 ctgtgtttat ctttcctact actgcatcat ttgcactagc tccacttctt acatttaaaa
     4261 aatcacactg gtctactgtt gcactcatag gtgaattagt tggctctgct gcactagccg
     4321 ttattgctcc tgcttctact gtgacaactc ctgctattaa caccatagct ataaattttt
     4381 tcaatatatg ttttttattt ttaatattca tcataatctc aaatacctaa tcctttcgaa
     4441 aatttaacat tttgttcctt cgtttccaat tcctaattaa cattctatct tacatcaaaa
     4501 taaattgcaa ccttttttgt aatctttttg taatcttttt gtaatctttt gtttcttttt
     4561 agtatatatt tgtttctttt tgctccccct ggttacaaat tagaaatata ttctactttt
     4621 ctctagttgt tgcaatttct ttgatattag catcttctgg tttaatataa gttggcacta
     4681 attccctcat aataccatct attttttcaa cctcttcatt gttagtaatt tctcttaaaa
     4741 gttttaagta ttttgttatt ttttctctat caacatctat tggtctacca ataaaaatct
     4801 ttttatgctc tgtatccaac agaccttctt ctgacataag tagttcttca tacaactttt
     4861 ctcctggtct aagaccagaa aattcaattt ttatatcaac atctggctca aatccactga
     4921 atttaattaa gttttttgct aaatccaata ttcttacagg ttctcccata tcaagtacaa
     4981 atatttctcc acctttagcc atagccccag cttgtataac taagcctact gcttctggta
     5041 ttgtcataaa atatctaata atatctggat gagtaactgt cactggtccg ccatcttcaa
     5101 tttgcttctt aaaaagtgga attacactac cattactacc aagcacattt ccaaatctaa
     5161 cagcaacaaa ttcagtctgg ctttcagcat tcatagtctg tataatcatt tcagcagctc
     5221 ttttagtagc tcccattatg ttagtagggt ttacagcctt atctgtagat ataaggacaa
     5281 atttcttagc tctatatttt gaagacaagc cagctatgtt taaagttcca aatatattat
     5341 tctttatagc ttcgccaggg ctcgattcca taagaggaac atgtttatgt gctgctgcat
     5401 gaaataccac ttctggttta tatttattaa atatttcatc cattcttttt tcttctctta
     5461 ttgatgctat tactgttttt aaatctaatt tatttttata ttttctaagc aattcttgtt
     5521 gtattgagta agcattattt tcataattat caactataat aagctcttta ggattaaatc
     5581 ctgcaatctg tctgcaaagc tctgaaccaa ttgagcctcc tcctccagtt attaggatta
     5641 cttttccctc aatgtaatta cttatctctc ttaaatttgt ttttataggc tctctaccca
     5701 acaagtcttc tatctctact tctctaatct gttttatatc aacttttcca tctataattt
     5761 cgtaaattcc tggtattgtc tttattttac agttagtgtt tttacatata tcaataatct
     5821 ctttttttct acgtttctct atgtttgcta ttgaaaagat tatctcatct acattgtact
     5881 tttctaccac atcttttata tctgaagttg ttcctacaat tttcacattg tggattattc
     5941 ttccaatttt atttttatca tcatcaatta tagccactgc tactttttta agttctggat
     6001 ttctttttag ttcctgtata accatttctc ctgcatctcc tgcaccaatt ataaggatat
     6061 ttgaaacctt tcctctagaa tatgtcttgt tcatgactat tcttatagct ctataggcta
     6121 ttcttgcgcc acttgtaaac attataataa atattgtatt taatacataa aacattatag
     6181 gataatcaaa ccctaataat ttatttatta tataaatcgg aattaaataa gctaaagttg
     6241 ccacaaaaat agatataatt tcttcttctc ctgcatatct ccataatgtg ttgtagcact
     6301 taaacaatct aaatgatact atatgaagta ctatatatat acacgctgaa attattatat
     6361 cattagtggt aaatgcattt aaattacctg tcaagcttaa agacattcga aacgctacta
     6421 gtacgctaca aatatctaga attaatagtg ttaaatgtct aaatttatat tttttaaaaa
     6481 gcctattaaa aaagctttct ttctctaaat ctatactttc tcgcataact agaagccctc
     6541 ctttaaacag caaaagctac gcacctaagc atagccaaca acatctatct tttgctgtat
     6601 tttttcattt tattttttat atttatcaaa tatagaatca taaatttttt ctatatcatt
     6661 aaagtttaaa ttaacctcat aattttctct aacaaattga cgccctttct tacccatatt
     6721 aagtctaagt tcatcatttt ccattagttt tattatagca ttttttattt cttctggttg
     6781 ttttggttct acaattaagc cagtttcatt ttcccataca gattctggat gtcctcctac
     6841 atttgtaact ataacaggta cttcacatgc ttctgattct atagctgcca ctccaaatcc
     6901 ctctcttaaa gaaggaaata cagttacatc aaaagaatta aaagtcttgc ttacattttc
     6961 tggagatatt cttcctaaaa actgaacatc attttctata cccaattctt tagctaaatt
     7021 tattaggttg tccatctgac ttccagagcc acctatttta agaattattt tcttatcttt
     7081 atattcatct ttaagcatct taaatgcttg tattaaatat tctattccat attttttttc
     7141 taaactcttt ataattccta ttacaaaagc gtcttctttt tctacttcca taggcttaaa
     7201 cctatccata tctactccaa aaaaggttac atctacatgt ttagctgtat atttattagc
     7261 ctctttagcc atatctctac tatttgaaaa gatatagtct gcttttctaa agttatgttt
     7321 tataatttgt ttttgtataa aaccattatt agggaaatca taaatatcag taccccaaac
     7381 agaaataata tatggatgat acccaagtag acttcctata aatccataac tacttgcata
     7441 ctgtgcatgc aatatatctg gctttatttc atttactagc ttctttatct gctttattga
     7501 acctaagtat cccgtttttt tagaaatatt ttcatttttt agttcttcta cattagaacc
     7561 aaaattataa atttttattc cttccatgtg acctccattt aaagagatta catgaatgtc
     7621 atatccttta tttttaaaaa aactacacca ttttaaagta tgaggatttg acccatcagc
     7681 taaataacaa acagtcttca taatatttac cttcctatcg aataatactc aattcctaac
     7741 tcttccatat ctttaatttt ataacaattt cttccatcaa ggactaaagg atttttcatc
     7801 ttttccttat acttatgtat atcataattt aatacatctt tccattctgt cattataaaa
     7861 catatctctg catcttctat cgcatcatct acagaatcag cataaagtat agagtcacca
     7921 aatattttct tagtattatt tacaccaatt gggtcgtata cagtaactct tgcccctctt
     7981 tcgagcaaat attttatatt tggtatagat ggtgcttctc ttatatcatc agtgtctggc
     8041 ttaaaagtca aacctaaaac agcaaccttc atatcttgaa agctctctac taacttagat
     8101 gcctttctaa ctaatttaag tttttgactt tcattaactt ctattgcagc ttttactgtt
     8161 tttaattcat aaccattatg ttcagataac cagtgaagag cttttgtgtc ttttggaaag
     8221 caagaaccac catatcctat tcccgccttt aaaaacttac ttcctattct atcatcataa
     8281 ctcattccta aagctacatc ttctatgtta gctcctgcta tttcacaaag atttgcaata
     8341 tcattcataa atgatatttt aagagctaag aagttatttg atgcgtattt tatcatctca
     8401 gcactatttc tatttgtaac tattataggt tgattaaaag gcttatagat ttcctctaat
     8461 atttctctag cccattcact ttctacacct ataacgattc ttgaagcatg tagagtatct
     8521 tttactgctg ttccttgtga taaaaactct ggattagatg caagttctac ttttacatca
     8581 ttttttaggt ttctatttat ataatcttct atctcttcat ttgtaccaat tggaactgta
     8641 gattttatta ctactagagt atcttttgtc acagtttcag ctatttgctt tgcaactgcc
     8701 tttatataat ctaggttggc tgaaccatca cttctctcag gtgtacctac agctattaat
     8761 attacatcta catcttcata agctttttta tagtctgtag tgtaatctat tcttcctctg
     8821 ttgtaatttt taaccataag ttcttctagg ccttcttcat atataggtgc tattccaagt
     8881 ttcattgatt taactttttg ttcatctaca tcaacacaga ctacattttc attgcctacc
     8941 tctgctagac aaacacctgt aaccaaacct acatatcccg tcccagctat agctattttc
     9001 atttttttac ctccaagtat ataaaactta tactcttttt ttaatggcat taaaaacata
     9061 gaaacaaaat acatatagac tttttggtaa acttaaattt tcaacatttc tataagtatt
     9121 ccaagttcgt tttaatgctt ttatcttgtt acttgatata gaatttccaa ctaatctgta
     9181 attcactaaa tcttcttgaa tcccatatgc atatttttcc tttttaagta actgtaacca
     9241 ggttgcagta tcttgtcctc ttcttaccaa aggcatgctg aactctccta caatctcctt
     9301 atcaatgacc actgtagaac atcctattat agtatttcta agtaatcctt catagtcaat
     9361 tttcttagga gctcttgcaa ccttattcgt ttttgaccca tcttgtctca tatatctata
     9421 agatgtaaaa gaaaaaccta cattttcttt taacatatat tctatctgaa tctctaattt
     9481 tctactatcc cataaatcat cactatctac aaatgcaata aatcgacctt ccgcattctt
     9541 aataccaaca tttctagata ctgcagctcc agaatttttt tcaagcttta tatattttat
     9601 tctagcatct tcttttcgaa aactttttat aatttcactt gaattgtcct tagaacaatc
     9661 atccactaat aaaagctgcc aatttttata agtttgattt tggattgact ttatcgtctc
     9721 tgataaaaac tcttctgaat tataaacagg agtaattatc gatactaaag gctcattcat
     9781 atttttactc tccttatata gcaactagca gtactatacc aacaagttta cttagatact
     9841 aattttcttt gtccaatata tttgtaatat cctaatggga ataatgctaa atacaacaat
     9901 ttatcagata cttttcttaa ttcaccaaaa taatgactat cagacaacaa tgagtatctg
     9961 gctaaattta tgtaattttt tatccacata tatggatatc tacttaatgg atatgtattt
    10021 tgatttatta aatataaaaa ctctaatgct ctacctctta atgcattttt acttaatata
    10081 ttcttagtca aaccatcatc caaataatct cttattatca taggtttatt tacacatata
    10141 actttatagt atttagacat ttcattccat actacacttt ctggtaaaaa tctcacttct
    10201 tttctttctg gaaaaggaaa ttttataagc tcatcagtta caaatactat gcttttatct
    10261 cccttaacgc catacttaaa atataggtct gcaaagtgac atattttctt atcttcaggc
    10321 atttctgtcc ctatcaaatc tccacttgga tatacacaaa gtccaacaac tccactaaat
    10381 ccttctttat tctctaaaga atcccactca tcattagcaa tttcaagaga atctttaatt
    10441 aaaccatcat cactatctac aatgaaaaat agctctccat ctgcattttt tacacctaga
    10501 tttattgcag tatgttttcc tccattttct ttgtatatat atcttatatt taatttattt
    10561 tctgatataa atttttcaac taactcttta gtactgtcgc tacttccatc atcaacaatc
    10621 aaccattcaa aatcattgaa atcataagtt tgagctttta aatcattgta caaattttca
    10681 agtaaatgag ctctgttata cgtcggagta aatacagtaa atttatattt atacattaat
    10741 taaatcttcc tctctgtcta actttttttg attccgttta ttccataatt aataaagtag
    10801 tatatagctt tgtataaagg tattttttct atatttctat atatattcca agtccacata
    10861 gcagctttta ttttattacc acttagggaa ttactagatt ttctatataa tgctagaact
    10921 tcatttattc catgagcaat atgccctttt ttcaatatcg ataaccataa aacataatct
    10981 tcatgtctta caccactcat tcttatttca aaatccaatt tagacttatc aatcacaaca
    11041 gtaagacatc ctaagatatt tccttttaat aatcttctat aatctacatt aggtggaact
    11101 tttattactt tgtttaattt tttatcattt tcatccatca gttcatatga agtaaatgaa
    11161 attacatagt cattttctaa cataaaattt acttgttttt ctaacttact actattccat
    11221 tggtcatcac tatctaaaaa agctataaat tgtcctgttg ccttacttag tgctaaattt
    11281 ctagcattag agacaccctt attagtctca gtctttatac attttattct actatcctgt
    11341 tgcatataag atttgactat attaggacta ttatctgttg agcaatcatc aataattaac
    11401 atttcccatt cttgataagt ttggtttaat actgatttta tggttgcttc aataaatttt
    11461 tcagaattat acatgggagt aattatagat actaaattct tcttcatatt accttcctca
    11521 tcatttataa aatatttatt taattttact taatacacaa tcatatatcc tcttgcagtt
    11581 cttattatca ttatatctga aaaacttatt tctttcattt ttttggttat ccataacctc
    11641 aaaattattt tcaataatat caataagttt attcacggct tcatcattat tataagccaa
    11701 agacccaaac atttcacttt ttaaatctat atatgaatca actttttcca tatattcatc
    11761 caaatcgaat tggtaaaaca aaataggtct attcatatac aaaaagtcta tggctacact
    11821 tgaataatca gttatatttg cagctgattt tctgatttca tttgctagag ttaccccttt
    11881 atctaaaaac acaattcgtt ttcctgttat attttctttt atatcatcta taaactcatg
    11941 cataagatga tgcatataaa tttttacatt tatatctttc tcatctaata ttttatttaa
    12001 tttgtcatca tttaaaaaat tcattatatt tttaaaataa tctgtatccg taaattttcc
    12061 tgaactaaat tttatccaat ctctccatgt aggagtatat aatatatctc gagtttgtgc
    12121 aacttcattt gttggtaaat tgtcatatct tgcatttcca attatataaa ctgagtcatc
    12181 taacattccc caatcattta ccataacatt tctttcaaat tcagaacttg ctgtaactat
    12241 attatagctt ttatataggt tatctataat atttttgaac tttttgttca taggttttct
    12301 ttttttaaaa cttatagtcc catgatttaa aaacactttt atcgtttttt tatgaaaata
    12361 attaagaaca ggtacagcaa aattgtatgg tgctatatct gctgatggtg catgtgaaaa
    12421 tactatagct tttgaattgt aataatacag atagttttct acacttcctc ttattaattt
    12481 tttgcctgga attttatcaa atactttact gtctttattt actacccaat aagtttctac
    12541 atcattatgt tcttttagca tatactcata aaaaaacttt gaattatcat tgtatatatc
    12601 ccctgaatgt ccacctataa gccagatatc tctaccatta aacttatgct tattaaatgg
    12661 atatataaaa aaagccaaaa gcatatttat taaaaattta accttgtcca taaacaccat
    12721 cctcattata acttgtactg tctaaatcca ttaattcttc cttttatagt ggcactatga
    12781 taatctctat tggaactcaa tataagacct cctatatttc ttacaagttt tgataccata
    12841 acttttgcaa acttataatt ttttctatag actatttcct ttttgcaaag cgctccaaag
    12901 cctcttccat agttataatc tttttgatac ttttctttag atttagaatg tttttttgca
    12961 ggatggtaaa ttatatcatt tccaaagtat tttcctttaa aacctaaact caataagttt
    13021 aaaacataat ctatctcttc accagcacca aactctccac caacacccaa tttctcatca
    13081 aatcttgtat agtctttacc ttcaaaatca ataaaaaaag ttatggatgt tatagtatcc
    13141 aaaacgttgg aacttgtgat atcacaagtt ccatcataca ttttcttaaa tgcatcaact
    13201 ttatttgaat ccatagtttt gcaactgtat attttataat ctttattttt attaaagaaa
    13261 tttataactt tttctaatgt atcattttcg tagacacaat catcatcagg gaaacctata
    13321 atttgcccta cagcattgtc tatgcctaca tttctgttat aactcaatcc ttttttagag
    13381 ctttttatat actttatatc taacttatca atgtatttat caactatttc tttaactttg
    13441 ctattatcat tttggtctac tactataagt tcaaagtttt tgtaagtttg attttctaag
    13501 ctgtccataa gtaagtatat atcatcatat ctatttaatg ttggcattat taatgatatt
    13561 aacattacta cctcctactt ttcatcttcc acaaatctat ttccctttta tcatttaata
    13621 tcacttcttt tattagtaat actactgcaa aactaaatga agcttctaat aataaatttt
    13681 ctgcaaaaga gtatagtaaa aatataaata aagctactaa atatgctttt ttatcttttt
    13741 ctataaagct ttctattcct ttatacatca aatacataaa taatatacaa gcaactactc
    13801 ctagtacact aattatatat acataagaac tatctaatgg attagttgct tttatagcag
    13861 gactatatga atttccaaaa ggttttagaa aattcccctt ttcagccaag tatacatgcc
    13921 agaacttagg tctacttgct aataatctat ttaataaagt gcttttatca aatactgtac
    13981 caataattac actaaataaa gtaagtatta tcggtaatgt ctttaggagc attggtagtc
    14041 cttttatttt ttttatatct atccatctta ttatttctac aaatattaat cctgctaata
    14101 tagtgaaaaa tccagttcta ctatatgtgg tttgataaac aaaaaatgca gaaccaaata
    14161 aaattattct atcccattta ttataatctt tatatcttaa gaatatatat gctgcatata
    14221 tagtaaccat atgataaaat acactatttg gattaccaag tccaaaatcc attcttgtaa
    14281 caagcatatc tattttttct atatatctat agtggacttc ctgattaaat atcaatgaag
    14341 gaaatagatt aaataataat atagctacca ttaaaaaaca tgctgatatt attaaataat
    14401 ttttaactat aaatttatca tctatttgct tagctgatac agctaataaa acgattaata
    14461 atatagctcc ttccctattt aataaataaa gtaagacaca aactccaact agtaccattt
    14521 ctgttatact ttttctcttg tcaaataaaa aaagtagcat tagtattcct attgaagaat
    14581 aaaataacgc tgatttaatc atccctatat ccatatttgg gtcaataaat gatatcatat
    14641 actttctaag taatatgata aacaaaacaa atatataaag atattttcta atcataatat
    14701 ttctatcatc atttaaagtc ttcattttac tttctccctt ttagcttcgt aaagtattac
    14761 ttgcaaatat tttacaacac ttccatatct tatttacacc tgttaagtgt ttgctttcta
    14821 agaaataatt tctcttttta gcccaacttt ttccccacca atgtatccca aaagtgtttt
    14881 ctgttataca ttctggtgta aagtcttctt taaatccaaa aggataaaaa tattctttgt
    14941 cgtaaacacg tattccttct tcataattat ttgggtcaat cttcttataa ttcttttcca
    15001 taagatgagt cattatcttt ggtattgtaa acaatgaaga agtaagtact tctttttcat
    15061 aatactccaa taagtctttt aaaaaaggat gtcctttttc tacacctatt atagcaccat
    15121 ttatatactc tctatcatct tcatacccac agattagcct attattttct aaaagaggag
    15181 ttatatcctt taatatttgc atatctgtat ccatataaac tccaccttgt tcatagagta
    15241 cttttattct tgtataatca gatatgaatg cccaaagttt tcttttataa cattcttcca
    15301 aaaacttatt tccttttatt tctttctcta tatcaaagtt tttttcattc cactctacta
    15361 tctcatattc tggaagcttt tctttccaag aatttataca tatattttct atatttcctt
    15421 ttggaccacc aaaccataca taatgtattt ttttaggaat caaatgaaca cctcctacat
    15481 atattcttgt ctatcttctt cttttaactg ctctactatt tttttgacat cttgagcttt
    15541 atcttttcta caaaccataa ctgcatcatt tgtagatact actatcaaat cactaatacc
    15601 tattgttgat attaatttgt catctgaata tataattgaa tttttagtat ctatagtaat
    15661 attttcacct cttttgatat taccttcatc atcagttgga tatattgcac ctagagaatc
    15721 ccaagaacca acatcattcc atccaaaatc accaggaact actataacat cattacttct
    15781 ttccataata ccataatcta tagagattga ttgtatagtt gggtatattt cttttatttt
    15841 ttccatttct tcttttgttc ctaagtattt acttatatct tctaattttt catatacttt
    15901 tggcaagtat ctcttaaaat cttctagtat tttagatacc ttccatacga acattccact
    15961 attccataca tactttttag agtttacata ttctttagca atttcatagt ttggtttttc
    16021 tacaaactcc actacctcat atgcaacatc ttctatagta ttttctctat taaaatttat
    16081 gtatccatat cctgttgatg gaaatgtagg agttatccca atagttacta gtttatcatt
    16141 attttctgct atatagatag ctttttccaa aatagattta aattcttttt catcttttat
    16201 ataatggtca gctggatata cacacataac accatcacca tattttttca ttatgttaaa
    16261 tgctgcaaaa cctatagctg ctgctgtatt tctggcacat ggctctggca atatattact
    16321 atccaaacat ttatctttta ctatatcctt taaagcttcc aattgcttct cattggttac
    16381 tataaataaa tcatcttttt ttgctagaga atctattctg tttatagttt cgtttattaa
    16441 agcatcttct ccacttaaat ttataagctg tttaggtact tcttgtctac taagtggcca
    16501 gaaacgagtt cctcctcctc cagccattat gacattgtat actttcatta tttatctccc
    16561 cttagctttt tatatagaat ccactacatt taatatataa gatattaaat tgttcaactt
    16621 aaattctaca tcttcttgat tgtctgaatt tgctccaaag taaaatttaa ttttaggttc
    16681 tgtacctgaa ggtcttatag ctatccaaga gccatcttca agtaaaaatt taagtacatc
    16741 tgacttaggt aaatcatcaa caccatcttt atagtctaaa attttatcta cttttacatc
    16801 agccacatta tctattttaa cacttctaaa gtaaagcatc atctctttta ttttttttat
    16861 tccatctatg ccttttaaag ttactgactt taagtcctct ttgtagtatc caaatttatt
    16921 atatgtatct atcaaagctt catacaagtt catacctttt gaagaatagt atgctgccat
    16981 ttcacatatc ataagtgaag atacaacacc atctttatct ctagcatgag ttccaattaa
    17041 gtaaccatag ctttcctcat atcccattat aaaacttcta tcattgcttt cttcaaacgc
    17101 ttttattttt tctcctataa atttaaatcc tgttaaagta tttaaacaat ctacattata
    17161 agcctttgct atttttgcac ctagttctga tgtaactata gtttttatta ttgtaggatt
    17221 gtttttagga agtttatttt cttcaactaa actctctaat atatatctaa ctaaaagtga
    17281 accgacttga tttccattta agactacata ttcaccactt gaatctttta caacgatacc
    17341 tactctatca caatcagggt ctgttcctat tatcaaatca gtgccattgt tctttgccat
    17401 ttcaatagct atattaaaca ctgacttttc ttcaggatta ggatacttta ctgttgaaaa
    17461 attagaatct ggcaattctt gctccttaac taccattaca ttttcaaagc cacattcatt
    17521 taagactttt cttactggct tatttcctgt accatgaata ggtgtatata ttatttttaa
    17581 ttttttcccg tattcatcta ttatattttg tcttaaaact tgcttcttaa cagcttttat
    17641 aaactcatca tctacttcat tatctaatat agttattaaa tttttagata aagcttcttc
    17701 aaaatctata cttttaatag tactgtaatc atcaatctta ttaacttctg ctattatatc
    17761 atttgccata tctatacaga tttgaccacc atctgagtca tatactttat atccattgta
    17821 ttctttagga ttatgacttg ctgttattac tattcccata gcacacttta aatatctaac
    17881 agcaaaagaa agttctggtg taggctttaa ttcttcaaaa atataagcct ttacaccata
    17941 agcacttaaa gtctttgcta cttctataca aaactcccta gacatgtatc tactatcatg
    18001 tgctataact attccctttt gcttaccttc ttctccttga gttttcatga tataatttaa
    18061 aacgcccagt gtcgcacgtc taacagtata aatattgatt ctattagttc ccgctcctat
    18121 tatacctctt aaaccacctg ttccaaactc taaattttta taaaatctat cttgtatttc
    18181 tttttcatca tcttttatac ttaatagctc atttttcgtt tgttcatcaa aataaggaga
    18241 atttaaccac atctcataat tattcttata atccatattt tcatcctttc tgactctaat
    18301 ttaaaatttc aaggtaatta tctatcttct aagtagtttt tgttttcctt ttcttaaaaa
    18361 ttcaaaagct aaatccatct cttctacctt aaataaaact atcaatacag tatatacaga
    18421 agctccacct aatactccag ctccaactga tataatttct ttaattgttc ctgaacccat
    18481 aaatgcatat agattattat atatcaataa agtagctact gccatcacac cagatgctac
    18541 caaacttttt agaccagtct ttataatttt atcccctcca aaatatccat ttttcttttt
    18601 aagagatata aataataata taactgtaat tatgtttgat gtactagttg atatagcaat
    18661 acctgcatga cccaatggtc ttatcaatat taaattaaga actatattta ttattaaagc
    18721 tatacttcca tttatcattg gcgtttttgt atctgaaaga gagtaaaatg ccctatataa
    18781 gacatctctt actccacaag ccaataaacc tagtgaatat aatctaagcg ctatagatgt
    18841 aaggtctgtt gataacgcat caaatttacc cctttggaat aatattctaa caagaggctc
    18901 tgctaatata atagctccta tagatatagg aagcaccacc aatataatac aatttgaaga
    18961 ttttacaata gtacttataa atccttcttt attatcttta ccagacattt ttgctagttt
    19021 aggatatata actgttatta tagatgtaac aaataatgcc ataacaaact catttagcct
    19081 atttgcatag ttaagagcag acaactttcc atcacccaaa gtggttgcca ataatcggtc
    19141 aacaaatata tttaactgtc caacagctac tcctaccaac ataggcaata ctaggttgac
    19201 taactctttt atggactcat cctttaaatt tatatatggc ttatacttgt aggacttctt
    19261 aaacgcaaat ggcagttgaa acaatagctg acttgccata gccaatagtg ctcctgctgg
    19321 cagtatccat acatttttac ctgcacttag tgctattgct gctattatta taatattata
    19381 tgggattcct ataagcccag gaattacaaa actgtcattc acctgtaaaa acgaactaaa
    19441 tatcttactt ccactcagga ataaaactcc tgaaatcatt attttagtaa ataatattgc
    19501 tgcttggaac ttagcagggt cgtttctaaa tcctgctgca aatactgtaa caaattgctc
    19561 tgtaaataaa agaccaataa tagctattac tatagccatt atatagcata tatttaaaac
    19621 attattagta aattttaagg caccttcatc tccgagtctt ttctttgtct caaagtacat
    19681 aggtatataa gttgtagcaa ctgcagagcc tatgactgct atgattaaac caggtatatt
    19741 taatgttaat acaaatacgt ctgcatacat acctgtccca tagaaatttg caagtacaag
    19801 ttctctaagg aaacctaata cttttgaaaa catagtggct gccataatcc acaatgctgc
    19861 tttagctgtt tttgacatct tctttctcct tatctaattt aaactctaaa agtagaactt
    19921 ttaaaactgc ttataaacat tattctaatt aagtaaattt aacctatgaa aagcttcctt
    19981 ataaatcaaa aactatttcg actcatatct aagtgcccta aatgtaatca atagtaataa
    20041 tagtattaat actatacaag ataagaatgt tagattaaat atgctcttaa atagactata
    20101 atcacctact gtatacccaa cttcaactac atcatttata ttgctatctt tcaagaattt
    20161 atcttgtgca cgacttaagt tatcactaca aatcagtatt ggcatatttt cttttgctga
    20221 tactactccc aaagaaatat attcgtttac atcagatcta ctaccaaagt ctaatctagc
    20281 agtatagatt ttctttatat ctttttcatc ataaaacttc tttattaaat caacatttgt
    20341 acttgactta tttatttgct ttattccttc tatatttttt aagttagaat caatctctgc
    20401 aataaactct tttgaatcac ctaatgcata tattttttta agatttttgc cttttaagaa
    20461 atcaatttgt tcctcaaacc cttcatcttt agcccatatt attggcattc cacttttagc
    20521 ggcataagaa tatacagata tggcgttctc taatcctgtt gttgagctaa gtaaaaatgc
    20581 ttcatcaaat cctactgttc tatacatttc tctagctatc tttaaagatg tatcatatct
    20641 gtctttccct cttattcttt ctactttcat tcccattttt tctatattac gttcagcatc
    20701 tttagatact gcctttaaac cacctactat cgtaaccttc tctggaccta gttcttttag
    20761 ataatttctt gttactctac caagattttt actcttagtt acaactattg gagcattttt
    20821 tgcatatgct aaaggtgttg cacttatact atctacaatt gactcttcat ttacaagaat
    20881 tacttcttta gaatccttaa atgctctttt atttaattct gtcgaaatct ctggtatact
    20941 cccttctatc aattctcttt tgggatttgc atctgcaaat actcctatcg tactagaaat
    21001 agagattaac aaaactaacg ataatatagt agaaattttt ttaatcataa gttctcattc
    21061 cttttctttt ttgtattttt tgacaataaa aaagctcttt ttaaagagct ttttcattga
    21121 ttagtaatct aatattttaa aattatctag ctccgtctcc tgtcaatact actttaacag
    21181 ttttaaagag tatcttaaag tcaagtaata tactcctatt ttttatatag taaatatctt
    21241 ctttaagttt ttcttcagga gtgatttcgt atccaccatt tacttgtgcc caacctgtaa
    21301 gcccaggttt tatagcgagc ctgttgataa atccaggtat ttcctcatta aattgaacag
    21361 taaagttcgg tctctctggt ctaggaccta ttaagcccat gtcccccttt aatatattaa
    21421 acagctgtgg aatctcatca attctagttt tcctaataaa ctttccaacc ttagttattc
    21481 taggatcatc tttttcagcc cattgtgcgc caaatttttc tgcgtctgtt ctcatagatc
    21541 tcaatttata tatatagaat cttctcccgc attttcctag tctttcttgc ttataagtta
    21601 ttggtccttt gtcctctatt ttaataagaa ttccaaatat tgctattaaa ggtaaaccta
    21661 tcacaagtcc tattatagat aaaactaaat ccataattct ttggtacaag tcgaataaaa
    21721 ctgttccttt tactatgcta tagtcaatct cacttgcaat ttctttatct gcccctggat
    21781 agcttttaat tttaggtatt tcacactcat cattagtata tccagacata gttacctcca
    21841 tattggaata aaaatttttt taattcttaa tttcaactta tttctatctt atttttgact
    21901 tatttctata ttatactcaa taatgagcag attatcaata caagaaacat cattgtaaac
    21961 aatttgattc tttacaaaaa tatatcatct cttgttatat tttacattat aacaggagat
    22021 gatatgtttt gctttaatat tttctatttt attttactgt atcttctatt cctattccaa
    22081 tttgagttcc cattgttttt actttttctg cagtatttaa cttatccata tcgctcttat
    22141 tgtcaataaa tgcacattca acaagtattg ctggataatt attatttctt aacacatata
    22201 ggtaatcttt tccattatca agtgttcttg tcttgattcc tctatttttc atattaaatt
    22261 tttctagtat tctttttaag atatttgatg ctgcagtctt agttgttcca ccatttttat
    22321 cttttacttt gtaataaatt tccacaccat taccagatcc attagacgca ttgtaatgta
    22381 tacttgtaaa taaatctggc tttatagtat ttgataatgc tgttctttct cctaaagcca
    22441 ttgttttatc tgtatctcta gtcataacaa catttatacc ttttgaacgt aaatattcag
    22501 tagttgctaa tgctgtatta agagtatatt tcttttcttg tgctccacca tttaatccac
    22561 ttgttgcccc tgaatcagaa cccccatgac ctgggtctat cactacagtt tttcctgctg
    22621 ctgaacctga attatctggt tctgttggtg cattgtgttt tgataaacta gatgctatag
    22681 aatttataac actatctttc attccatcac caatttgata tactgtttct gctgtttttt
    22741 ctgataagtt tgtactatgg tatgcagaaa cataagtttt tggagttata agtattggag
    22801 cttttaattt agctgctaaa ggtcctgctg ttattgaatc aactattatg tcagattttg
    22861 caactaacat tgcactcaac tctttatctg gatagaaagt cttaattaca tttgcatttg
    22921 tttcatgtct atctgctcca gaaactctat tttttgaagt accatttttt gctattttag
    22981 atatttggtc tataattttt gatgataaac tttgactacc acctatgtaa taagcttctt
    23041 ctaaaccttg tgaggataac caattatatg taccttgtgg tacagaatct ttattagcta
    23101 atattatagg ttgttggtct tcaccagctt ttgatgaaat attaagagca tcatattcac
    23161 cagcatatcc atttgctatg tatattttat taacatcatg atacttatct atttcttttg
    23221 ctataagtaa agatgtttca tgtctatcaa ctccaccaat acgagtcaca tttacattta
    23281 cagcagattt aatttgagac actgcctttt gtgaaactga accatcatca cctataatta
    23341 ttacatcact tggatttaga cgttttaact ctgcttttgt actttctggt atatcatctt
    23401 tatttgcaag taatattggt gcgtcatatg tagatgcaag tggagtagct gtaattccat
    23461 ctgctgctaa ttctccatta actattacaa ccttactaga cccatctttc caaccttgtt
    23521 tgcttatctc aacagcagtt tcaaatctag tagctccagc aagttttttt actggtgcag
    23581 aagtttttct catatattca tttactgctt ttgttgaatt tgcatcactt attataaact
    23641 cttgtgtaac atcataatcc ttaagattta atcccataga ctctaagtct tctttgttta
    23701 aaactttctg ttcatcttta ccatttattt ttatgctttg aagttcccat acatttaagt
    23761 attctgggtc tttgttatag tttattacac cttcatatga atctgtcttt tcattgtaat
    23821 ttaaagtagc ttcaagtggc atatcataac acataaaatt taatgttatt gaatcagcat
    23881 ttaatttttc tttaaatttt acacttgcat tagatttact tcctaacact acactttttc
    23941 tttgtaaatt tatattaata tctgaattag attctgtatt tgcagtagta ttttcagccg
    24001 ctaaagctac atttggtgcc accattgcta cagacaacat tcctgttgcc aataattttg
    24061 ttgttttttt catcatatcc cccctaattt tttggttctt taaatcttta aatctttttt
    24121 aatacatctt aagcttgttt tatattacat aaattcaata ttatgtaaat cttttttcat
    24181 tatagcacaa aaaattacaa attgtaaact ttttgtaatc ttttatggca tggtttttaa
    24241 attttatcga ggagaaattt tgattatatt tgatagtttc cgtatatttg aacaaatcaa
    24301 agactttatc atctaaaaat attgcactaa attttaataa atattttttt cttgtacatt
    24361 atagacatta ctataaccca gaataggaca tacaataaag aaaatataag agaatcccaa
    24421 gttgtacctg cccaaggtgt tataagttca tatgtagtcc actctaccaa ggtagttgga
    24481 taatcgactt tgttcgtaag ttttggaaca ttccaaaaaa catgactcaa tatttcaagg
    24541 cacatatatg taaaaatagg gctacttcca agtgctatta ttggggtaaa tattttactt
    24601 ttatttttga tgtcgcatat aaaataaaat attgacagta atataccata actgccagcc
    24661 attaaaagta caaatgagct agaccataat cttttattaa atggaaaata ctgattaaag
    24721 ataaaagcac ctattagcag gattatgctc ataaccagta ttttaaagaa ttttttatat
    24781 tctcctatgt tttcttttaa aagtacacat cccatagtgc atcccaacat acctgaagaa
    24841 attgctacta tactagttaa aaaaccatct ggttcaaatt ccggcttgta caaatgtccc
    24901 ttgaagaaat gtaaatccac taactgtgca agagaacctt ctagctcaaa tccataaggt
    24961 tttgctaata tgtagtaccc aactattata aatgtagata tacatagaaa agtaattatt
    25021 gtagcagtac ttccaacatt taattttttt agtagtaaat atactaaact agtcacaaaa
    25081 tagactaatc ccatacgttg aagtactcca agtattctta cagtatctaa atcaggattt
    25141 cctaggtaat ttaagaaaaa tccaaataat atcaataaga tacttctctt gaatatacta
    25201 agtattatac tcagtgtgga tttattattt tttagtttac tatttattga tattggaatt
    25261 gtaacaccta aagatataac aaaaaaagga aatgcaaaat ctgcaagtgt tacaccatgc
    25321 catacagcat gtcttaattg aggatacatt ctcatccaag tcccaggatt attgcagact
    25381 atcatgaggg ctattgatag ccctcttatt atatctattg atttaatcct actattcata
    25441 agtttagaat ctacagcatt atttgaaat
//
LOCUS       DAYATN010000045        25120 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.045, whole genome shotgun sequence.
ACCESSION   DAYATN010000045 DAYATN010000000
VERSION     DAYATN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.045"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            113..1969
                     /locus_tag="ACGI8A_001768"
     CDS             113..1969
                     /locus_tag="ACGI8A_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0179766.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGTYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    2052..2292
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2358..2593
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2678..3895
                     /locus_tag="ACGI8A_001769"
     CDS             2678..3895
                     /locus_tag="ACGI8A_001769"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFH0179767.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            4012..4494
                     /gene="cdeM"
                     /locus_tag="ACGI8A_001770"
     CDS             4012..4494
                     /gene="cdeM"
                     /locus_tag="ACGI8A_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HFH0179768.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            4758..6023
                     /gene="hisD"
                     /locus_tag="ACGI8A_001771"
     CDS             4758..6023
                     /gene="hisD"
                     /locus_tag="ACGI8A_001771"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFH0179769.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6226..6978)
                     /locus_tag="ACGI8A_001772"
     CDS             complement(6226..6978)
                     /locus_tag="ACGI8A_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0179770.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7096..7365)
                     /locus_tag="ACGI8A_001773"
     CDS             complement(7096..7365)
                     /locus_tag="ACGI8A_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HFH0179771.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7840..8934
                     /locus_tag="ACGI8A_001774"
     CDS             7840..8934
                     /locus_tag="ACGI8A_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFH0179772.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8936..9886
                     /locus_tag="ACGI8A_001775"
     CDS             8936..9886
                     /locus_tag="ACGI8A_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFH0179773.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9879..11063
                     /locus_tag="ACGI8A_001776"
     CDS             9879..11063
                     /locus_tag="ACGI8A_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179774.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            11076..12572
                     /locus_tag="ACGI8A_001777"
     CDS             11076..12572
                     /locus_tag="ACGI8A_001777"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFH0179775.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12595..13545
                     /locus_tag="ACGI8A_001778"
     CDS             12595..13545
                     /locus_tag="ACGI8A_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0179776.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13566..14528
                     /locus_tag="ACGI8A_001779"
     CDS             13566..14528
                     /locus_tag="ACGI8A_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFH0179777.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14900..15532
                     /locus_tag="ACGI8A_001780"
     CDS             14900..15532
                     /locus_tag="ACGI8A_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0179778.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15958..16035
                     /gene="kdpF"
                     /locus_tag="ACGI8A_001781"
     CDS             15958..16035
                     /gene="kdpF"
                     /locus_tag="ACGI8A_001781"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFH0179779.1"
                     /translation="MWFLAVVIIMLIIYLVYALINPEKF"
     gene            16082..17758
                     /gene="kdpA"
                     /locus_tag="ACGI8A_001782"
     CDS             16082..17758
                     /gene="kdpA"
                     /locus_tag="ACGI8A_001782"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFH0179780.1"
                     /translation="MVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDNFI
                     YKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGLSF
                     NTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKKKT
                     LGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGPVA
                     ALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKNKK
                     QGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSSLF
                     TTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVFLC
                     GLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGFHG
                     LSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGVVMFFGRYVSIIVLLAISSLLASK
                     KAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17783..19840
                     /gene="kdpB"
                     /locus_tag="ACGI8A_001783"
     CDS             17783..19840
                     /gene="kdpB"
                     /locus_tag="ACGI8A_001783"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFH0179781.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            19859..20485
                     /locus_tag="ACGI8A_001784"
     CDS             19859..20485
                     /locus_tag="ACGI8A_001784"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFH0179782.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20722..21630
                     /gene="cysK"
                     /locus_tag="ACGI8A_001785"
     CDS             20722..21630
                     /gene="cysK"
                     /locus_tag="ACGI8A_001785"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFH0179783.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21657..22247
                     /gene="epsC"
                     /locus_tag="ACGI8A_001786"
     CDS             21657..22247
                     /gene="epsC"
                     /locus_tag="ACGI8A_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFH0179784.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22407..22595
                     /locus_tag="ACGI8A_001787"
     CDS             22407..22595
                     /locus_tag="ACGI8A_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFH0179785.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22649..23518)
                     /locus_tag="ACGI8A_001788"
     CDS             complement(22649..23518)
                     /locus_tag="ACGI8A_001788"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HFH0179786.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23715..24959)
                     /locus_tag="ACGI8A_001789"
     CDS             complement(23715..24959)
                     /locus_tag="ACGI8A_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179787.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDGLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9679 a         2679 c         4517 g         8245 t
ORIGIN      
        1 taaatttatt cacaagtata tcttataaaa tatgttataa ttaatagaaa attgtcatat
       61 aattaattaa gtagtattat tataaataac attgaaaggt agtggttaga atatggatac
      121 ccataataaa tatgtaaatt ttataaaaaa tatacctgta ccttttttat attgtagaat
      181 tgtaaagaga caagaagata tagaatatcg agtagaatat ataagtaagg gtatgggcaa
      241 agtattacaa ttagaagaag gtatttgtga taaaaatata ctagatgtat tgcctgtatt
      301 taagtctaaa aaatatttca aagagttgtt ctcaaatgaa gtggattgta taaaaaggta
      361 cattccgaca cttaaaaatt ggataaatat aaaaaaacag ataatagggg attcatacat
      421 aattttgtat tttggtaaaa ttgtatttga ttatagacag ataatcgatt cctttgataa
      481 aaaggaaaaa gtagcttata ttaaagacga agaaggcaca tatatagatt gcagtgaaaa
      541 tttaatacca atattaaata acaacattaa aacaacgaaa gatatctttg gtaaaaatga
      601 tatagaagta tggggagaga atacaggaaa attatttaga gatgattata gagaaggagt
      661 atctagtaaa aaaagatttt tacaaaatct ttttgaatat gaagaaacat tttttatggt
      721 tgaaaaatat tttttgtatg atgaagacga acttttaggt actataggga tagtagacaa
      781 tataatatat agtggttaca gtaatagaaa ttataactca aaagatttaa tgaagatgat
      841 agaacactca attccagaga atatgtttta taaagatgtg tatggaaatt atattggatt
      901 taattcaggt tttttgaatt tagcttgtat gaataaagaa gaattattag ggaagaatag
      961 ttataaaata tcagaagaag aagctttaat agataagata tttgaaagtg ataagggtgt
     1021 agttgaaaat aaaaaagtag ttacatttga gttaaatata agtatgaatg atgaaaacaa
     1081 gtgtattgag attacaaaaa ggccgttttt tgatagctat ggaagtgtta ttggaataat
     1141 tggaacagca agggatataa gtagaagaaa aagattagaa gaggaaatgg ataaaactag
     1201 gatggaattt tttgcaaatt tatcccatga acttagaacg cctataaact taatatcatc
     1261 ttcattacaa gtgatagaaa aaaaagaagc agatttaata gaatcaaatg atactttaaa
     1321 aagaaattta ggtataataa aacaaaatgg aaatagaata ttaagattag taaataatgt
     1381 tatagatttt acaaaaatgc aatcaggata tttagatttt aaaccagaag aatctgatat
     1441 aattgcattt atagaggaga tatgtatgtc tgtagctgat tttgcaagcc aaaataatat
     1501 acaacttaca tttgatacag aaatagaaga attttcaatg ttatttgatt cagaaaaatt
     1561 ggaaagaata atattaaatt tactttctaa tggaattaag tacaataaga aagatggaaa
     1621 aataaatata tttttatatg tgaaagataa tgtatttaat atgaaaatat ctgatagtgg
     1681 aataggaata ccaaaagaaa aaatagataa aatatttaat aggtttgaac agattgacaa
     1741 tgaactttct tacagagtta aaggaagtgg tattggactt tcattagtga aatctttggt
     1801 tgagttgcat gagggaagta tatctttaaa aagtcaactt ggtattggaa gtgaatttat
     1861 agtttcattg cctgttagaa gtaaaaataa tattgaaaaa tataatcata aaagagaaat
     1921 cagcaatgaa ttaagtaaaa aattagaaat agagttctcg gatttataag tttaaatata
     1981 aaaaataaaa ttttaaaaaa gtattgacaa ttgatttaga agggtttaaa ataaattaca
     2041 acataataaa taaaaaggcg atgaatagga agagtaaatc taattcgatt accagagaga
     2101 agagtccgtt ggctggaaga ctttttaaat tagagggatt gaaaactacc taggagctac
     2161 tttctgaagt catctgcatt agtagatttt agtaagaata gtcgttagct gcgttaaagt
     2221 gtaaagtgag ttttcatgac atatgaaaac taaattaggt ggaaccgcgg gaataatact
     2281 ctcgtcctta tgttataagg atgagagttt tttgttattt aaaaatataa acagtaatca
     2341 taataattat agaaaatata aaagcaatga ataggaagag taaatttaat tcgactatca
     2401 gagaggaaag ttcacaggct gaaagacttt ctaagtttga gaaattgaaa actacctagg
     2461 agctgtggtc tgaagttact ttaaaaagtt ttagtaagac taaccgttaa ctgcgttaaa
     2521 gtgtaaagtg gattttatca ataaatgata aaatcaaatt gggtggaacc gcgagtatag
     2581 actcgtccct ttatataggg atgagtctat ttttattgca ataaaagatt gaaaataaaa
     2641 aaaaatcgga aaattataaa tttaggagga aaataaaatg gagaataaag tgaaaattgg
     2701 agtactagga tatggagttg taggaagtgg acttatagat ataatagata ataataaaga
     2761 aaaaagaaat atagaaatag ttggaatttt agttaataat ttggaaaaac ataaggataa
     2821 aaaatattct aatataataa ctaataatat agatgatata tttaataaag atatagatat
     2881 acttgttgaa gtcatgggtg gtttagaacc atcattatct tatataaaaa aagcattaaa
     2941 caataaaatt catgttgtta cagcaaataa agatttgtta gctgaatgtg gagatgagct
     3001 tgcaaaatta gcaagtgaaa ataaagtaag tattaaattt gaagcttcag ttgcaggagg
     3061 tataccagta ttaaaaccaa taatagaatc actagaagga aataatatag atagtataaa
     3121 tgcaatttta aatggaacta ctaattttat attatctaaa atgtatgatg agaatctatc
     3181 ttatgatatg gcattaagac aagcacaaga attaggtttt gctgaagcaa atccagagtc
     3241 agatgtatta gggtatgatg ctgctagaaa actatcaata ttatcaactc tagcttatga
     3301 caatagagta tattggaaag atgtgtattt agaaggaatt acagatatag atgaaaaaga
     3361 tattgaatat gcaaaaaaat taaattgtaa aataaaattg attggtcaaa gtaagtatga
     3421 aaatgataaa gtaagtgctt ttgttagacc tgttttagta gaaaaagata atatccttgc
     3481 tagaatagac aatgagttca atgctgtcat agtgaatgga gattcagtag gagaagtttc
     3541 ttttgtaggt aaaggagctg gaagtttagc aacaggaagt gcggtttatt cagatgttat
     3601 agatataata gataatagag tttcaagtat agattctttt actaaagata aaatacaagt
     3661 taataaaata gttagagaaa agtgtggagc acttcttaga tttaaaaaat gtaataaaga
     3721 tgaaattcta aatatagttg agaattgttt agtaaaattc gatattttaa atgatgatga
     3781 tgaacttgct attatggttt atgctgattc tgaatatgaa ataaacaaca gtttatgtct
     3841 aataaaagat aaaggatact gcgaaaaaat gaataaaatg ttgaaaataa gttaaaaaag
     3901 aaaaaatata agtttatatg taacatattt tgacgatttt aatactatat agtatgagat
     3961 gaaacaaaac atctcataaa aaaattaaaa ttaaagggag agatatgtta tatggaaaat
     4021 aaaaaatgtt attcagaaga ttggtatgaa agaggagaat ctacagctaa atggttccaa
     4081 aatgatagag aagaatatga aagagaagca tatgatgaag atagagaaag aagaggttca
     4141 aactgtggat gttcagattc aggagaaaat agacctagaa actgtgaaag atttagaaga
     4201 gaagctgaga taagagaaag agaagcaaga gaagcattct gtgaatcttc agagaaaaag
     4261 aaagaggcat tagcatatga atgtgaagct agaaaattat gggaagaagc agaaaaatac
     4321 tgggatgaat attcaaaata caactataaa ggaatcgaat atttagcaga agctgctaga
     4381 ttatttgatg aaggtatgga atgtgaagct agaagaaatg gaaataatgg aggaaacaat
     4441 aataattgtt gccataaatg ccataaatgt aattgtaact gctgtagaaa ataaaaaaat
     4501 taattgacaa atcaatagtt aaaatatatc atattatgta taataaagag aaaattcaga
     4561 aaaaaataat gattagaaga aatataagta tatttattag acaaatagtg ctcagttagt
     4621 gaggtgagta caatatgatt aaactcccat cctctagctt ttgatagagg gtgggagttt
     4681 ttatttattt atatatctta aatgaggata atattgtaaa ataaatgagt atgagattgt
     4741 aaaattagga ggtaaaaatg aaaagtatta aaacatcaag tatgaaagaa aaggtagatg
     4801 aaaagatatt aagagagcaa gtaagttcta taatagaaga tataagaaat aataaggatg
     4861 ttgcacttaa aaaatataat gagaagtttg atagaaatac aagagatgaa tttagaatta
     4921 ctaaggaaga aataaaggaa gcttataagc atgtagatga cgaatttata aataacttaa
     4981 agattgctgc caaaaatata agagaatttg ctaaagctca aaaactaagt tttgaaaacc
     5041 cttttgaaaa agaaatttat ccaggtgtaa tcttaggtca aactaatatt ccaatagagt
     5101 catgtcttgc atatgttcca ggaggtcagt atccattgtt ttcaacagct cttatgctta
     5161 taatacctgc aaaagtagca ggagtaaaaa gaatagtggc atgttcacca actatgaaaa
     5221 atactgaaaa gataaatcct aagacattgg tagctatgga tatagcaggt gctgatgaaa
     5281 tatatgcaac tggaggagtt caagctatag cagcatttac atatggaaca gaaaagatta
     5341 atccagttga cataattgta ggaccaggaa ataagtttgt aactgaagct aaaagacaat
     5401 gttatggaca agtagggatt gattttgtcg ctgggccaag tgaagttctt ataatagcgg
     5461 atgaaacttc aaatccagtt tatattgcag ctgatttatt agcacaatgt gagcatgatt
     5521 taaatgcaag aggaatatta ttgacaaata gcttggaagt agcacaaaaa gttgaaaaaa
     5581 atatagaaag tatgctaaaa gatttgccaa caaaggacat agcatatagt tcatgggaaa
     5641 ataatggaga gataatattg gttgatgata tggaagaagc cattaaaata agtaattttt
     5701 atgcaccaga acatttagaa gttgcagtaa atgagtataa tgatatttgt gatagattaa
     5761 ctaactatgg ttctttattt attggaaatc tttctgctga ggtgtttggt gactatgttt
     5821 caggtaccaa tcatacatta ccaacactta aagcatcaag atatactggt ggtgtttggg
     5881 ttggaacttt tatcaaaact tgtacaaagc agatatttaa tgaaaaagct atacaatcat
     5941 tagcacctgt agctgaaaag ctggcaaaag aagagggatt atatgcacat gcaaaggctg
     6001 ctgaggttag gtttaaaaag tagtaaaaaa ttttattttg gcagtcgaga cttagtatta
     6061 gactgagata aggaaaacgc tgagaacctg aataaatact atattttgtg ggtctcggcg
     6121 cttcttttat atctgtatat aatagaataa tactatctgt gtgtttaaat actaacttaa
     6181 tatttctttt ctaaataatg tttatgaaat agttaatatt tttagttaat acttgaaaaa
     6241 aacttttgct aactgtgcaa attcatctgc atttgtcaat atagcagggt gtcctccctg
     6301 actaaaaatg taaatacttg cttttgaaat aaaagttgcc atttgcttgt actcttcttc
     6361 aagattatga cgacacattt cgtcttcctt acttcctgta aataaaattg gcatttctaa
     6421 tttacacaat tctttatgaa acaaagggcg tttctcgttt gcacattgca atagagcttt
     6481 tgtatcaaga ttaacaacat tctcccaatc ttttccatga caccattcat agaattttcg
     6541 tgcttgaata tcttgctttg atttttccct tccagtgatt agattatcta tgaagttatc
     6601 attaagagta cgtccatcaa agctatctgc tataattgct tcaactaaat cgggacgttc
     6661 taatccagta ttaacagctg cccatgctcc tccacttgtt ccaattaagc caactttacc
     6721 acaattcaaa tgttcaatca gtgctattgt ctgtaaggct tcatcatgcc atatgtcagg
     6781 agaaaattga gaaactctat ccgactgtcc attacctaaa aaatcaatta agatacatct
     6841 aaaattttct gcatataatg gcacaagtgc tttaaacata ttagaagaac cagtattacc
     6901 atgcaggaaa attatcggtt taccttgtcc atattcttta taaaaacaag aattaccttg
     6961 atactgaaaa tatgacatat attttcctcc tttttatttg ttaagttaat tataacaata
     7021 taggtatgaa tgaaaaacct catgttcttt cttagaataa aaatgtgcca cgcataaaac
     7081 gtgacacatt tctaattata aattttgctg tttaagtatt ctttgtatgc ttttctctgt
     7141 caaaaagtat ttctctgata actgcttgat actcattcca ttttggaaat ttatacagat
     7201 ttgagaatta cgagaagcta agacttgttt ggtgtttgtg ttttcacccc aatgcttttt
     7261 gtgctcttgc tttttgggaa tataaatcac tttaccatct atatattctt gcacttgctc
     7321 aatcagttct ttaggcagta agtcaatcga attttgatag ctcattttgc acctcctaaa
     7381 taattattct ttaggattag caaaggctac tgccactcac aattaaaaat aaaatttttt
     7441 attatgtaca atagcctttg ctatgcacaa gttccaattc ccataaacta aatactcctt
     7501 tctggtttga aaaattcatc tgaacattga tttcgctttt aagatagagt tcaatgacaa
     7561 atttacattt ttattatata tattcagata gataattgca actgtctttg tatgaggata
     7621 ttttattata gtcaatacta taatatgtat ttatatcatc aaactaatat gaacagttga
     7681 aaggaatatg aacaataaaa cttagtcagg aagaataatt atgaagcaga caatagagaa
     7741 atttgatttt aaagttttat tgaaaattta ttgacaaatt taacttgtaa atattatact
     7801 aaacttaata aataaagttt ataaagtaag gtgttgtcta tgaaaaatat agttggaaaa
     7861 ccgcaaacga taaaaaaagt aaatgaagat ttaataaaaa acattataaa aaatgaaggt
     7921 cctataacaa aaccagaaat ttcaagcatt actgaattga gtttagctac agttaataag
     7981 attgtagagc aattagcttt gaaaaatgaa gttaaagtaa gtggactaag tgaatctact
     8041 ggaggaagaa gagctcaatt atttgaaata aatgcaaact tagaacacat aatagcttta
     8101 tattattata gaaattgttg tataggggta gttgccaatt tattaggtga aataatttat
     8161 caagaagaat ttaatataag aatggatacg tatgaaaacg ttaaccaaga tacttttttt
     8221 gttatagata ctctaataaa aaaggaaaaa ggaaacaata taagggctat tggaattggt
     8281 gtcccaggag ttgtaaaaaa aggtgtagtt aaaaatatac caactataaa aagttgggaa
     8341 ggtataaatt tagaaaattt aataaagagt aaatacaata ctaaagtgtt tatagaaaat
     8401 gatacaaata ttactactat tgggatttat caaaaacagt ataaagacaa atataaaaat
     8461 atggctctta tgtatttaga agatggtatt ggttcaggta tagttataaa tggtgaattg
     8521 tatttaggtt ctacaaattt tgctggagaa ttaagttatc taaatataga atttggtaag
     8581 tataactcta acaacttaaa tcttgaagat tatattatga gtttaagaaa aagatattta
     8641 gaaagtaaag ataataaatt taagaaagaa attttatcta taatatcaaa ctttataagg
     8701 actcttgtat gtgtcctaaa tccacaagct atgattatac agtgtagtat attaaccaag
     8761 agtgacatgg agtttataga aaaagatgta agcaataatg ttggagaaga aaattttcct
     8821 aaattaataa aagtagactc attgagagaa ggaagtataa atggagtaat tagtatgtgt
     8881 ttaaaagaaa ctgactatca atattcctta tctaataaaa aaggaggata ttaatgtgaa
     8941 atatttggtt tcaatagatg gagggggaac aaaaactaaa ttttgtgtaa gtgacttaga
     9001 tggaaacata cttaaagaac ataccactgg ttcaactaat tataagtcag ttggaattaa
     9061 aaaaacatat gaaaatatta ataatggatt taaaaaaata ttaaaagatt tatatatcga
     9121 ttatgacgat attgagtaca ctgtatttgg tatatctggt tgtgattctc ctaatgatta
     9181 taagattata atggatgaaa tattaaaaat tgggattaac aaagaaaaaa tatatctggc
     9241 aaatgatgca gttttagctt tttatgcaca agctgattcc cctggcttag taattgtggc
     9301 tgggacaggt tcaattatac tgggaattaa agaagatgga gaaatatata gagttggtgg
     9361 atgggggtat aattttagtg atttgggttc tggatatgat atagggagaa aattattaaa
     9421 gaaagtattg ctttattgtg atgaatgtca tgagtatagt gatttattta gttgtgtctt
     9481 ggacttcttt ggagcaaata gttttgaaca acttgcatat atgattactg atataaatga
     9541 taatgttgaa atagcaaact tagcttcatt agtgattgat tgtgccgaac aaggtgataa
     9601 attggctatt gaaatattaa gagaatcatc aactgaacta tctaaattag ctcaagtaat
     9661 attgagaaaa atatctaatc caatgaaaat ggataaaagc gaaataaata tagttttgtc
     9721 tggtggtaca ttaaataaaa ctgtatatgc tcaaatgtta atgaataatt taaaaacaga
     9781 aaatacagat aaaaatttaa aatttatatc tcaagaaaat cagccagtat atggggggat
     9841 aagacttgct atggcattag ctaataggag ggtcaagcat ggatagaaaa aaggtaatag
     9901 tacaaatttt tactggaggt tttaaggcta aagaaataaa gtttaatgat ataaaatgta
     9961 aactactaga attattggac tgtattgata tagaaaaggt tatcatggga tggagtgtag
    10021 atgaagaatt atatgtagaa actaaaaaac tattaaataa atataatgtt gaactatatc
    10081 tttggttgcc atgtttttct gaaataggat tacttgaaaa aagtaattta ctaattgatt
    10141 atatggggaa ggaagtcaag ggctatgctt tacaagagga tgaaaatttt gagttttatt
    10201 gcccaaataa taaggaaaat ataaaaaata taaagaaaat atatagtact tattttagtg
    10261 atattaaatt tgatggaatt tttttagata aaataaggta tggttctttt tcgaataaac
    10321 taaatggtgt ttttaattgt ttttgtaaag attgtttaaa tatatataaa aaaaataata
    10381 ttgataaaga gtcgttaatg agagaaatga ataatgtgtc taagggagtt atagataatg
    10441 aagatactcc atttggtata acagaataca ttaatggaaa atacaaattt aaaaataaaa
    10501 tatgggaaga tttttttgaa acaaaaaatc aaaatatata caaggcatta ttagagataa
    10561 gtagttattt taaaagtaag ggattgaaaa taggtataga tgctttttct ccttttattt
    10621 cttactttac aggtcaagat ttagaaaaat tacaagaagt agcagatttt ataaaaccaa
    10681 tgatgtatag aactacaaaa gctccagctg gtttaccatt tgaatttgaa aggtttattg
    10741 atgaatccat acataagaat aaagagattg gaaaaagaaa tttcttagat attttaggaa
    10801 ttaaggagta tgaaggagat gtatatccta tagaatttgt actagaggaa atcgatttta
    10861 tgacaagaaa tcttaataaa gatatatatg taggagttga aataaataaa aaggaaatta
    10921 tatctcctgt tacacctaag tacataagtg aaaatatttc aaatataaat aagacaacag
    10981 taaaaggata tgttttatct tgggatttgt tgtcagcacc aaaagaaaat attgatgaaa
    11041 ttataaaatg gtttcggaat tagtgaggtg ataaagtgag taagatattg gaaatacaac
    11101 aaatatataa aagttttgga aaggtagatg ttttaaaagg gataaatttt gagttagaaa
    11161 agggagaagt aagagctctt ttgggagcta atggagcagg aaaatctaca cttattaaaa
    11221 ttttaggagg agttcattct caaactaaag gtaaagtaat tttgactggt aatgaaataa
    11281 tgtttaaaaa tccacatcat tctaaagaaa atggtattag tataatttat caagaattaa
    11341 gcttgatacc aacgcttaca gtacttgaaa atatttttct tggtagagaa attttaaaag
    11401 gaagtgtgtt tttaaacaag aaagacatgc ttgaggaata tatgaaaatt tgtgaggatt
    11461 ttgattttaa tatagaccca catatagtag tttctaattt aagtgtagca aaccaacaaa
    11521 tggttgagat aatgaaagct gtttcatgta attctgaaat tatcataatg gatgaaccta
    11581 caacatcact tactaataat gaaaaagatg ggctatttaa aataataagt agactaaagt
    11641 ctaaaggtaa aacaatagtc tatatttctc acatattaga ggaagttttt aaagtatgtg
    11701 atagtgcaag tataatgaaa aatggaatta tgattggaac ttatgatgta gaggatttaa
    11761 ctaaattaaa gataactcaa cttatgacag gaaatgataa tcacaaactt attgagaaga
    11821 aggattatac atttgcaaaa tatgaagagc caccagtact agaagtaaag aatataagct
    11881 ggtctaacaa aataaaaaat gtttcattta aagtttatag aggcgaagtt gtaggtcttg
    11941 caggtcttgt aggttcaaga agaactgaaa ttataaattt gatatttggt atagataata
    12001 aagatagtgg agatattttt atcaatggta aaaaagtaga cataacttct cctaaggttg
    12061 ctataaaaaa caaaatagga ttgattcctg aagatagaaa aaatctagga ttaattttag
    12121 aacaagctat atacacaaac tcatcatcag tacaaattga taaattcaag aaatttttgt
    12181 ttatagatag aaataaagaa attgagtttt ctaaaaaagg tgttaaggaa ttaggaataa
    12241 aagttagtga tgtaaagcaa gaagtaaaag agcttagtgg tggaaatcaa caaaaagtag
    12301 tagtttctaa gtggttagag aaagatttag atttaataat atatgatgag ccgacaaagg
    12361 gaatagatat tgctgcaaaa gaagatattt tcaatacaat caaagaattt tcaagtaaag
    12421 gaataggagt aatatttata tcttctgatt tagaagaagt aataagagta agtgatagaa
    12481 ttttagtaac tagagaaggt tctattgtag atgatttaaa aaatgagaat atacaggttc
    12541 aagatataat gaacagtata tttaatgtat aaaaaatgaa aatggaggac tgagatgaag
    12601 aaagcttttg agaagttaaa tttaagagat tatggagtat tattaggttt tttagcttta
    12661 tgtatagcta taagtattgc aactccatca tttttaggta aacaaaatat acttaatctt
    12721 ttacgacaat cttctataat tggaataatt tcagcaggta tgacttttgt tataatatct
    12781 ggaaactttg atatatcagt tggagcagta gcagctcttg caggtgctat aacaatgaaa
    12841 tttgcgactg gtggaactaa tttgtttgca tccatgttct taggtgtagc gatttgtgca
    12901 gtaataggat taatcaatgg tatagtagtt gcaaagataa atgtaccatc attaatagcc
    12961 actatggcta tggtcactat tgtaagagga ttgctgttga tgcttacagg aggatatcca
    13021 attactgaaa atataccaat acttgattac attggaaatg gatatttgtt taatatacca
    13081 ataccagtag taattttctt tatagtggtg ttagcttcgt ttattgtact taataaaact
    13141 aagtttggta gatatgttta ttctgtagga gggaatcaag aagcaagtaa acttaatggt
    13201 ataaatgtag attctcacaa agtgaaagtg tttattatta atgctgtact tgcaggaata
    13261 gctggaatcg tactaacagg aagacttggg actgcaactg cgattgcagg tgaaggatat
    13321 gatatggatg caattgcatc tgtagtaata ggaggtacat cagtggcagg aggttctggt
    13381 tctgtactta aaactgttat aggagtatta cttatgagtg ttataaacaa cagttttaat
    13441 cttcttggaa tagatgtata tttccagtac atatttaaag gattaataat tttagcagca
    13501 gttggatttg attcttatag caagaaaaaa cttgcttcaa gataaaaaat aagggggatt
    13561 tttttatgaa aaaattaatg aaacatttag cattgttgct atcacttgtt atgatatttg
    13621 gtttagttgg ttgttcaaat ggtggtgaca aagaaaaaaa tggagaaaag aaaatagcag
    13681 tattactacc aggttcaaca ggttactttg tagcaactaa acaaggtata gatgcaaaat
    13741 ctaaagaatt aggtgtaagc gttgaatatg cagatgctca atgggatgct agtaaacaat
    13801 tatcacaagc tgaagatttt atggctaaag gggtagacat gattgcatta tgtggagttg
    13861 attctgctgt aagtgagaga atagttaaag ctgctaatga ttcagacgtt cctatagtag
    13921 catttacaaa ttctatagga agtaacccaa ctggagaatt taaaggttta gttacatata
    13981 taggacaaaa tgaggaagaa acaggagcat taactggaaa aattgctaaa aacttattag
    14041 gagaaactgg tgggaaagct gtattaatag aaggagtacc aggaactact ccacaagtat
    14101 taagaaaaaa aggtcttgaa aaagaattaa aagatagtaa tatagaaata gtttataatc
    14161 aaacttcaaa ttgggagaaa gagcaagctt taaaagtaac agaagattta attcaaaaga
    14221 aaactgattt taatgttata atatgtcaag atgataattc tgctacaggt gcaggtcaag
    14281 ctttaaaaga tgctggtctt aaggataaag ttaaagtcat aggtttgggt ggaagtaaag
    14341 atggattaaa agctataact gatgggttaa tagatggtac aacttatatg tctgctgtag
    14401 aagaaggtgg attagtcata gaaaaggcat ctaaattcct taaaggtgaa aaaatagaac
    14461 ctgttacaca aattaaacaa gttgaagtta acaaagataa tatttcagag ttcaagggtg
    14521 aatggtaaat tgtcatagat aataaataag atttttagag ttactttaga aactatataa
    14581 agtaactctt tttttaattt aatttagtga taaattattg aatagtattt ctaaacaaat
    14641 aaaaaattaa tataaacaaa gacttaatta tactaaatta taagtaaaac taatataaac
    14701 ttatggaaaa aaggaaaacg tctaaagtat tggaattact atagtgtagg tgtagtatta
    14761 aaaaaacata aaaaaacata aaaaaattgt tgaaaaaaat atagaaattt tgtataatta
    14821 aagatttgaa aagtgcggta tacaaagcca attcttttgt atataatata tataaattca
    14881 gaaaaaggaa gggatatgta tggccaggcg gttattttta gaaaggattg gacttatatt
    14941 tttaggttca gcaatattag catttggagt ttataatttt tattatttaa acaacataac
    15001 agaaggtgga gtgttaggta ttttactact gttaaaaaat ttattcaata tacaaccagc
    15061 aatagcaaat gttgtaatag atggtttact acttttagta ggatataaat ttttcggtaa
    15121 aaaattcctt atatattcaa tagtagcttc tataactttt tcagtgttat atgacttatt
    15181 tgaagctata ggacctctag taccacaatc acaaaatatg cttttatcaa ctattttggc
    15241 aggtgtaact gttggaactg gtgtaggtat agtagtaaaa gcaggttgtg catcaggagg
    15301 agacgatgct ttagcattag taatttctaa gactacatct ttgaacatag ggcaagttta
    15361 tttagcaaca gatgttattg tattgttgtt atcattattt tatctatcag cgtttgatat
    15421 cttttattct ttgattgctg ttacaattag tggcaaggta attgatttta tttattatca
    15481 tggtaaaagt ttggatatgg atataagtaa tgatatagtt ccagaatgtt aatataaatt
    15541 aaataacttt agattttatt aggggtggca gatttgccac cttttttgtg tctaaatctt
    15601 tatagaaagt ataatcccat ctaggttttt gcaaatctca aatatagaat tatgttggaa
    15661 tgactgggaa tacgattgct agatgtgcat tttaaaaacc caattttata tttatgaaat
    15721 actaatcatt aatggatgtt tcttaatcct atctttatac ctaaaatgat aagataaatg
    15781 aagtagcaat acaaaactaa aatttgtaaa agagattaaa ttaaatggag gagctaaaat
    15841 gttggatata gttatgatag cattgttatt cataggattt gtttccatga agttacttgc
    15901 tgattggtgt gggaaacaaa ctgaaaaaaa ataatgtaat gtatggaggg attacctatg
    15961 tggtttctgg cagttgtaat tataatgtta ataatatatt tggtatatgc gcttattaat
    16021 cctgaaaaat tttaaacagt ataaggctaa taagtaagtt gaattggggg gaacaatttt
    16081 tatggtgcag atagtaatag tgttagcgat ttttatgatt ttagtaatac ctatgggcaa
    16141 gtatttatat catatagcta ctaatcaaaa aacatttgga gatagactat ttgataaagt
    16201 ggacaatttt atatataagg tatgtagtat agataagaaa aaagaaatga actggaaaca
    16261 gtatgcatta gctttattat ttacaaatgc agttatggta tttataggat atatcatact
    16321 tagaactcaa agtatgcata tttttaatcc aagtggaatt aaaagtatgg aacaaggact
    16381 atcatttaat acaataataa gttttatgac aaatacaaat cttcaacatt attcaggtga
    16441 atcaggactt tcatacttta gtcagatgac agtgataata tatatgatgt ttacatcagc
    16501 tgcaacagga tatgcggctg ctatggcatt tgtaagaggt ctagtcggta aaaagaaaac
    16561 attgggaaat ttttatgtag atttaattag gataacaaca agagtacttt taccaggagc
    16621 tttaattatt gggcttatat tagttacaca aggagtacca caaacttttg ctgggacaga
    16681 aacagtgact actatagaag gaaagcttca agatatagct agaggtcctg tggcagctct
    16741 tgaatcaata aaacatttag gaacaaatgg tggtggattc tttggttcaa attcatcaca
    16801 cccatttgaa aatccaacaa taatatcaaa tatagtagaa atattatcta tgatgatatt
    16861 accaggagct tgtgttgttg catttggtca catgataaag aataaaaaac aaggttgggt
    16921 tgtgtttggc gcaatgtcaa tcatattttt aataggtcta gtagtgtgct ttaaagcaga
    16981 aagtgcagga aatcctattt tatcacaatt agggcttaat caaagtatgg gaagtatgga
    17041 aggtaaggaa gttagatttg gtatagcaca atcatcatta ttcacaacag tgactacttc
    17101 ctttacgaca ggtacagtta ataatatgca tgatacactg actcctttag gaggattagt
    17161 accactttta aatatgatgc taaatgtagt atttggtggt aaaggcgtag gtctgatgaa
    17221 tatgttgatg tacgctataa tagcagtatt cttatgtgga ttaatggtag gaagaacacc
    17281 agagttttta actaagaaaa tcgaaggaaa agaaatgaag cttattgctt tacttattat
    17341 attacatcca cttttaatac taatgttctc tggtctttca gtagcaatac cagcagggct
    17401 tgaaggaatt tcaaatcctg gattccatgg attatcacag gtattgtatg agtttgcatc
    17461 ttcagcagcc aataatggtt caggatttga aggtctagga gataacacta tgttttggaa
    17521 tataacaaca ggtgtagtta tgttctttgg aagatatgtg tctattatag tactactagc
    17581 aatatcaagt ttgttagcat ctaaaaaagc tgtaaatgaa agtataggaa cacttagaac
    17641 ggataatttt acatttacaa tagttttagt acttgttgtt ttaattgttg gtgctttaac
    17701 atttttccca gccttagcgc ttggacctat ttcggagcat ctagtgttgt ggcactaaaa
    17761 aattgggagg gagtaaagtt ttatggaaac taaaaaaaca aagtttatta caaaagatat
    17821 tttcaaaact tccattatag aagcttttaa aaaattaaat cctaaataca tgatgaaaaa
    17881 tccagttatg tttgttgtag aaattggatt tatagtaaca ttgatactta caatagtacc
    17941 tagtttgttt ggtgatgaag gcaataactt aagagcttat aatgggattg taacaataat
    18001 cttatttata acagtccttt ttgcaaattt tgctgaagct gtagctgaag gaagaggtaa
    18061 agctcaagca gatacactta agaaaactag aaaagataca gtagcaaggg ttatagatac
    18121 gttaggaaat gaaaaaatga taagtgcaag tgagctaaaa cttggtgata ttgttttagt
    18181 aaatgctgga gaagttattc caaatgatgg agaagttata gagggtatag cttctgtgga
    18241 tgagtcagca ataactggag aatctgcacc tgtcatgaga gaatcaggtg gagatttcgc
    18301 atctgttaca ggtggtacta cagttgtaag tgactggtta aagataagaa taacaacaaa
    18361 accaggagaa tcgttcttag ataaaatgat agctcttgta gaaggtgctt caagacaaaa
    18421 aacgccaaat gagattgctt taaatacttt attagtaggg ttaacactta tatttatggt
    18481 tgttttaata tctctttatc caatggcaaa ctatgcaggt gtaaagattc caatttctac
    18541 tatgatagct cttttggtat gtttaatacc aactactata ggtggattgt tgtcagctat
    18601 tggtattgca ggtatggata gagtaactag atttaatgta attgcaatgt caggaaaagc
    18661 agtagaagct tgtggagacg ttgatactat gattttggat aagacaggta cgattactta
    18721 tgggaataga cttgcatcag aatttatagc agttggaaat gcagattcaa aagatttaat
    18781 aaattattct gttatgtgtt cattaaaaga tgatacacca gaaggaaaat ctatagttga
    18841 tttaggtatg aaattaggtt caacaggtaa gacaaaagag gcagaggaag ctgagtttgt
    18901 agaattttct gcacaaacta gaatgagtgg tattaactta gctgatggta gagctataag
    18961 aaaaggtgct tatgatagca taataaaaag agtaaaagaa tctggtggta cagtgccaga
    19021 agatttagaa gaaaatgtaa atcgagtagc taaactagga ggaacacctt tagtagtgtg
    19081 tgtagataat gagatatatg gaattatcta cttaaaagat acagtaaaat cagggcttgt
    19141 agaaagattt gctagacttc gagagatagg aataaagaca ataatgtgta caggggataa
    19201 tcccttgaca gcagctacaa ttgcaagaga agctggagta gatggattta ttgcagaatg
    19261 taaaccagaa gataaaatag atgcaataaa aaaagaacaa ttagagggta aaatagttgc
    19321 aatgacaggt gatggaacaa atgatgctcc tgcacttgct caggcagatg taggtcttgc
    19381 aatgaatagt ggtacaacat ctgcaaaaga agcagcaaat atggtagatt tagattcaga
    19441 cccaactaag atattggaag ttgttgaaat aggaaaacag cttttaataa ctcgtggggc
    19501 tttgacaaca tttagtatag caaatgatgt tgcaaaatat tttgcgatta taccagctat
    19561 atttactctt gcaatacctc aaatggagat attaaatgta atgaaacttt ctacgtcatt
    19621 tagtgcgata ttatcagctt taatatttaa tgctataatt atcccatgtt taatacctct
    19681 tgcaatgaag ggagtaaaat atagacctat gaaatcacaa gctatgttaa tgagaaatat
    19741 gcttatattt ggtcttggtg gagttatagt accattttta ggaataaaat taattgattt
    19801 attgattaca cctttagtaa tggttcttaa tttaggttaa taaatggagg atataaaaat
    19861 gaaaacatta aagccagcat tacttgtaag tattgtattg ttagttgttt gtggacttgt
    19921 atatccactt gttttgacag gtgtaagtca agttgcattt aaagataaag ccaatggaag
    19981 tatgatagaa gtaaatggtg taaaagtagg ttcagaactt attggtcaaa gctttacaga
    20041 tgcgagattc tttaagggaa gagtatcatc agtaaactat aatacttata caaaagaaga
    20101 tttagtacca gataaagatg gaaatacatc ttatggaggt gtatcatcag ggtcttttaa
    20161 ttatggagct acaaatccag aacttcatga tagagtacaa aaagatatag aaaagttttt
    20221 aaaggataat ccaactgtca aaagagagga tataccaaca gatttactta cagcatcagg
    20281 ttcaggactt gaccctaata taagtccagc ctctgcaaaa attcagatac cagctatagc
    20341 aaaagcgtct ggaatatcag aaagtaaatt acaaaaaatt gtagatgata atacttccaa
    20401 aaaacttttt ggagttttag gtgaagatag agttaatgta ttaaaagtta atgtagaggt
    20461 cgcaaaaata ttaggtctta tatagtattt tgtggaattt tagcaacaaa atattacgca
    20521 agatttaatt taaaggttaa attttaaaga gtgaaaatcg ataatgattt tcactttttt
    20581 atttgaatat tactaatatt tggaataaag tgattgtaag tataaaatcc aaaaaaaggt
    20641 tgcattatca aaatgataat actataatat atttatcgtt atcaatttga taatagattt
    20701 ggacatatag gaggaaatag tatgttatat aataacgcat tagagttgat aggaaacaca
    20761 cctgtagtaa ggctgaataa tttaggctgt actaatgggt gttctaacat atatgtaaaa
    20821 ttagaaaaag taaatccagc aggaagtata aaagatagag ctgtttatga aatgttagag
    20881 ggcttagagc aaagaggaga attaaaaaag ggagatgtac tagttgaagc aactagtgga
    20941 aatactggta ttgcattatc tatgatagga aaattaaaag gatatgaagt agtaatcgta
    21001 atgcccgaaa caatgagcgt tgagagaaga gatttaatga aggcttatgg agcaaatttg
    21061 gtgcttacag atggtaaagc tggaatggca ggttccatag aaaaagcaaa tgaactttta
    21121 aaagaaaatc caaactataa aagcctaaaa caatttgaaa ataaagataa tccaaatgca
    21181 cattataaaa caacagcagt tgaaattatg aatgatgtca aagatttaga tatattcgta
    21241 tgtggagttg gaacaggtgg tactttaata ggaactgcaa gatatttaaa agaacaaaat
    21301 ccaaatataa gagtaattgc acttgaacca aaaaaatctc cagctatatc agaaaacaag
    21361 actggacctc ataaaataca aggaataggt gctaactttg ttcctgaaaa ttatgattct
    21421 agtgtagttg atgaagtaat attagtagat gatgaagatg catttgatac agttaaatta
    21481 cttgcagcaa aagagggaat tttagtaggg atatcttctg gagcaaatat atttggtgct
    21541 atagagatgg caaaaaagta tccagataaa aagatagtta cagtagctcc agatggagtt
    21601 gataagtata tgtctatggg aatattttaa taattttatt tgagggagtg aatactttgt
    21661 ttaagaaaat aaataaagat attgaatata taatgaagaa tgatccagca gctaggtcta
    21721 agatagaagt ttttttactt tatccatcag ttcatgcaat gataatgcat aggatggctc
    21781 atgctcttta taagaagaag aagcttttta cagcaagatt aatatcccag atatccagat
    21841 ttatgactgg catagaaata catcctggtg ctaaaatggg ggaaggtata ttgatagacc
    21901 atggaatggg tgttgtaatt ggagagacag ctgaagtagg gaatagggtg acaatatatc
    21961 aaggagctac tttaggagct acaggaaaag atactggaaa gagacatcca actgttggtg
    22021 atgatgtatt aattggtgct ggaacaaaaa tactaggacc tttaaatata ggttcaaatt
    22081 caaaaattgg tgcaaattct gttgtcgtta aggatgtacc taatggtgca acagttgttg
    22141 gtataccagc aaaaatagta aaaataagaa acctagaacc tgtaaaaaag aataaaaaag
    22201 aagtgtccta tgagtatgat gaattagata atgtatatta tatataggat ttatataact
    22261 ataaaataaa ctttataaaa attattttaa aaggtatttt tttcaaattt gaggacataa
    22321 taaaaaagaa ctgaatgtta cagttttggt tcatcaatct ttgataaaca agattttaaa
    22381 tttgtagcgt ctaggaggat tttattatga aagcaaatgt taatcaagat acttgtatag
    22441 gttgtggatt atgtccatca atatgtccag aagtatttga tatgaaggat gatgggaaat
    22501 cacatgttat agttgatgaa gtaccaagtg atgctgaaga atcagcagca gaagctagag
    22561 aaagctgtcc agttgatgca atagatgtgc attaataaaa taaatgccta taaaaattga
    22621 attttcaatt tttataggca tttgtctttt attcagttag tatttcaaca cctgtttctg
    22681 taactaaaac catacattcc cattgagcag ataatttacc atcttttgta taaatagtcc
    22741 aattattttc ttcatcaaca aaaatttcgt gagttccttc atttaccata ggttctattg
    22801 tgaatatcat cccaggaacc aatagcatct cagtcccttt tttagataca taacttacaa
    22861 aagggtcttc atgaaagctt aatccaactc catgaccacc tatttctcta acaacagagt
    22921 atccattttt ctttgcataa gagttgatag catcacctat atctcctaaa aatccccatg
    22981 gtttggcaac ttcaagacct ttttggacac attcctttgt aacttgaact aatttttttg
    23041 cattttcatt aacattacct atcataaaca ttcttgaagc atctgaatag tatccattaa
    23101 aaatagtaga aacatctaca tttataatat ctccatcttt taagataatg tctttacttg
    23161 gaataccatg acaaacttca ttatttactg atgtacatac acttttagga aatccattat
    23221 agtctagtgg agcagggata gctccatttt gtagtgtaaa gtcatgaacc aacttattta
    23281 tatcttctgt agacattcca atttcgattt tatctgaaat ataatccaaa atttttgtgt
    23341 ttattacagc actttttttt atttcctcaa tttgactttc attttttata atatcatgag
    23401 ttggtacaat atgacctaac ttagcaaatg aatctatttt attatccata tccatatgac
    23461 attttttata ttttttagta cttccacacc aacagctatc atttctattt aacttcataa
    23521 cttttcatcc ttcttttatt atttatttaa attatgggtt acataaacta ttttaaaaca
    23581 taaccattat aaatattata acataaatac taagaaatcc tttatagata gtgatttatt
    23641 caaagtataa attattatat ggttattgtt gatgaaaaga ggatagttaa atgtattatt
    23701 ggatattagc gcacttaata gtttagttca agtttttcaa gaaaatcata tgttagagga
    23761 caattttcct ttaaagtatt ttttgaatct tcatcaagat aataatgtac aaatgattct
    23821 gcaaaaaatt cttctataaa cttgcttata tatgcatgtg caacttgctt agggtcataa
    23881 agcttatatc tttcctgagc aaatacttcc ataaattctt cagaatttga ttttttattt
    23941 aaaacatttt tatctatagc atgtgcagtt tcatgtaatt ctaaattttt tgatgaatgt
    24001 acatttgcat agctagggtt actataacct attttagcca ctatagaatt atttctatat
    24061 ataccaggta catctttcca agtcttgcct gatcttaccc aacatggagg taattgatat
    24121 tttaaatcac taaattctgg ttcatcagtt agatttgaat ttattaattt tatttttata
    24181 tccttttctt taagtgtctg taaatattta atatttattt ttgcaagtct gtttttcata
    24241 ttttcaactt catctttatc ataattcaag tcatcaggaa gtataagcat ttgttcaatt
    24301 atatctatac tactgtcttt tatattttca gattcttcat caaacttata ttgaagagta
    24361 ttattttcat agatagttat atttgtatta ttttctatag aataagagtg aatatcctct
    24421 aaatctaaaa tattcatttg ttgtaaaata tgttttgttt cgccactcca tactactaaa
    24481 gaaagtgaat taaaattaat tagtggattt tcttttgctt ttatatcatt gcttatatct
    24541 acatcaaata catattcaat tgagtcttct atgttaagac catctgtatt ctttttaata
    24601 tataaatacg cttgttttaa gtctatataa atctcatcta aagattcgtc ataaaattct
    24661 gataaattat tagtaaagag ttcttcactt aaaagttcat ttttatcagt tatactttca
    24721 attaaaatat taatttcttt tatcatatcg taaatactta tttttctatt tgctttttca
    24781 ctagatacat tgcatggcgc aagagcaatg caataaaaat ctttttcttt tttataaaag
    24841 gctttaagtg attttgataa aagtgtaaag tctacaccta tctgtaagtt gaccttaagt
    24901 gagtcatttt gtaataattc agtattattt cttttagctg tatttaaatt gttttccata
    24961 atcattgccc cctttttatt agggtatcat aattttttta tatgtgtcaa tttgtttttt
    25021 tgaaaattat aatttttttg aaaattttat tttttataat ttgtgaattt tttattatag
    25081 aaataaatat gttattatta tgtcaaaatt tagcattaga
//
LOCUS       DAYATN010000046        24879 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.046, whole genome shotgun sequence.
ACCESSION   DAYATN010000046 DAYATN010000000
VERSION     DAYATN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.046"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            178..546
                     /locus_tag="ACGI8A_001790"
     CDS             178..546
                     /locus_tag="ACGI8A_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFH0179788.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            549..920
                     /locus_tag="ACGI8A_001791"
     CDS             549..920
                     /locus_tag="ACGI8A_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HFH0179789.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1065..1520)
                     /locus_tag="ACGI8A_001792"
     CDS             complement(1065..1520)
                     /locus_tag="ACGI8A_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179790.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1559..2824)
                     /locus_tag="ACGI8A_001793"
     CDS             complement(1559..2824)
                     /locus_tag="ACGI8A_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HFH0179791.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            3294..4745
                     /locus_tag="ACGI8A_001794"
     CDS             3294..4745
                     /locus_tag="ACGI8A_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFH0179792.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(4964..5425)
                     /gene="ribE"
                     /locus_tag="ACGI8A_001795"
     CDS             complement(4964..5425)
                     /gene="ribE"
                     /locus_tag="ACGI8A_001795"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFH0179793.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(5530..6723)
                     /locus_tag="ACGI8A_001796"
     CDS             complement(5530..6723)
                     /locus_tag="ACGI8A_001796"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFH0179794.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(6746..7411)
                     /locus_tag="ACGI8A_001797"
     CDS             complement(6746..7411)
                     /locus_tag="ACGI8A_001797"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFH0179795.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(7445..8557)
                     /gene="ribD"
                     /locus_tag="ACGI8A_001798"
     CDS             complement(7445..8557)
                     /gene="ribD"
                     /locus_tag="ACGI8A_001798"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFH0179796.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(8689..8810)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8999..11482
                     /gene="recQ"
                     /locus_tag="ACGI8A_001799"
     CDS             8999..11482
                     /gene="recQ"
                     /locus_tag="ACGI8A_001799"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFH0179797.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      11571..11682
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11824..13125
                     /gene="thiC"
                     /locus_tag="ACGI8A_001800"
     CDS             11824..13125
                     /gene="thiC"
                     /locus_tag="ACGI8A_001800"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFH0179798.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            13179..13373
                     /gene="thiS"
                     /locus_tag="ACGI8A_001801"
     CDS             13179..13373
                     /gene="thiS"
                     /locus_tag="ACGI8A_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFH0179799.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            13366..14169
                     /gene="thiF"
                     /locus_tag="ACGI8A_001802"
     CDS             13366..14169
                     /gene="thiF"
                     /locus_tag="ACGI8A_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HFH0179800.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            14232..15002
                     /locus_tag="ACGI8A_001803"
     CDS             14232..15002
                     /locus_tag="ACGI8A_001803"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFH0179801.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            15019..16122
                     /gene="thiH"
                     /locus_tag="ACGI8A_001804"
     CDS             15019..16122
                     /gene="thiH"
                     /locus_tag="ACGI8A_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFH0179802.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            16123..16812
                     /locus_tag="ACGI8A_001805"
     CDS             16123..16812
                     /locus_tag="ACGI8A_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFH0179803.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            17000..18394
                     /locus_tag="ACGI8A_001806"
     CDS             17000..18394
                     /locus_tag="ACGI8A_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFH0179804.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            18911..20278
                     /locus_tag="ACGI8A_001807"
     CDS             18911..20278
                     /locus_tag="ACGI8A_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFH0179805.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            20303..20911
                     /locus_tag="ACGI8A_001808"
     CDS             20303..20911
                     /locus_tag="ACGI8A_001808"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFH0179806.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            20959..21330
                     /locus_tag="ACGI8A_001809"
     CDS             20959..21330
                     /locus_tag="ACGI8A_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFH0179807.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            21509..21847
                     /locus_tag="ACGI8A_001810"
     CDS             21509..21847
                     /locus_tag="ACGI8A_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFH0179808.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            22313..22816
                     /locus_tag="ACGI8A_001811"
     CDS             22313..22816
                     /locus_tag="ACGI8A_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HFH0179809.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            22863..23822
                     /gene="moaA"
                     /locus_tag="ACGI8A_001812"
     CDS             22863..23822
                     /gene="moaA"
                     /locus_tag="ACGI8A_001812"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFH0179810.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            23826..24296
                     /gene="moaC"
                     /locus_tag="ACGI8A_001813"
     CDS             23826..24296
                     /gene="moaC"
                     /locus_tag="ACGI8A_001813"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFH0179811.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            24332..24763
                     /locus_tag="ACGI8A_001814"
     CDS             24332..24763
                     /locus_tag="ACGI8A_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HFH0179812.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         9570 a         2766 c         4172 g         8371 t
ORIGIN      
        1 taataaaagt attttaaaat ctatatgtaa ttacttttat taataaaact caaagttata
       61 ttagtaaaaa ttggaattat ttataatata ctccaaaatt taaaaataat ggttgaaaaa
      121 tctaattaag agtactataa tttaattaag taattactta atcaaaatga ggtacttatg
      181 gaaaacttgg ttaaaatatt taaagcattg agtgatgaaa ctagacttaa catacttata
      241 ttagtatcca agagaaatat atgtcaaaag gggatttcta aatatctagg gatttcagat
      301 tctgctgttt cacaacatat aaaaatatta aaagatgttg ggataataac aggatataaa
      361 gaaggatact atgttttata tcatattaat aaagaatcat ttaatatatg tgtagatttt
      421 ataaattcta tgttaagtaa ttctagtgaa agctttatag atgtatttga tgtaaataca
      481 atacatctag gatgcagtaa agaatgtaaa tctataaaaa aatgttgtaa gagaagggag
      541 aaataataat gaaagtatgt ataccagtag aggagaataa aggattggat agtaaaccat
      601 atggacattt tggttctgct ccaatttttg ttgtgtgtga tttagaaagt ggagaagtta
      661 aatctttaga taatggagat ttagatcatg aacatggaaa atgtcaacca ttaaaagcat
      721 tgtcagggac agctgtagat gcagtagttg ttggaggaat aggacaagga gctataatta
      781 aattaaatgg aatgggaata aaagtttaca gagcagaagg tgatactata agtgctaatt
      841 tagacttact taaaaatgga aaattagtag aatttccatc agaccatact tgctcacatg
      901 acggatgtgg acatcattaa aaaaaaacag ctgatgaaat atatacttca tcagctattt
      961 tttcttttaa taaatacgat tttattcttt taaaaccaaa atttagattt ttatgttttt
     1021 tgtagtatcc ttaataatat ttcaagttta caagtttact acttttagat tttttttgta
     1081 tgtttttcta tatagtcaat atcatttaat atgtcattga gttctttatt aagtgttttt
     1141 aaatcatcac catctacatt gtagtttaaa aatagttcct ctacattatc aatgatagaa
     1201 ttgacttttc tagtaacttc aacaaatgtc ttaaagttac tactattttt agcatttaga
     1261 aattttttta aatcctttga tataaatgat actatttcat attcatcatt aaatagacct
     1321 ctaccaatag gatatggttc aattctctgt aaaaaatatt ggtctttagc attaatatta
     1381 taagtataag atatttccca accgtctcct aaatcaaact tgataaaata taatgtacta
     1441 cttacttttg atattcttgt ggcaccaagc tgtcttaaaa gaacttcaat cgaatcacct
     1501 tgaacagtat taatattcat atatacacat cccctttagt attattatac tatatttttt
     1561 aaaattttat aattaaataa acagtagata taataataga tataatcata agtggaaaac
     1621 ctattttgaa atactcctta aaagagatag gatgaccgtg tttattacct atacctgcta
     1681 gtacaacatt agcagatgct cctattagtg ttccatttcc tccaagacaa gctccaagag
     1741 atgttgccca ccatagtggc attacatcaa taccttcagc ttgcatagtc aaaataagag
     1801 gaattagtgt tgcaacaaat ggaatattat ctaagaaaga agagactata gctgacaacc
     1861 ataatatcaa caacattgta aatacaagat gtccttctgt aagtccaatc aatgcttgag
     1921 ccaatttgtt tattatccca acttctacta atccaccaac tactacaaat aatcccataa
     1981 agaataaaat tgtagaccat tcaatacctg ccataatttc atctacatct tgttttccta
     2041 ttaaaagcat tatagatgct cctgtcagtg ctacaacaga agaatctatg tggattgtac
     2101 tgtgaaacat aaaaccaaaa agtatgaatg ctattaatat aaggcttttt ataagtagag
     2161 gtttatcttt aacagatttt ttttcatcta gttttaatat tgcattttta gctctttcat
     2221 tcacaacaag ttcttttcca taaataaatc taaagcatat tatagttata gcaagtatta
     2281 ctataacagc aggacctaaa tttattacaa aatccataaa actaagattg gcagcactac
     2341 caatcataat atttggaggg tctcctataa gagtagctgt tcctcctata tttgaagcta
     2401 aaatctgtgt tattaaaaat ggaactggat taagtcctaa tatttgtgta ataactatag
     2461 tcattggtcc aataagtaag acggttgtaa cattatctaa tacagctgat aaaatagctg
     2521 ttataatagc aaaacaaatc attatcttcc atgggtcacc tttggctttc tttgcagtcc
     2581 agatagctat atattcaaat aaccccgagt ttttgacaac agctacaaat agcatcatac
     2641 caactaatac accaatagta ttgaaatcta tatggcttaa tccttcatca agtgtaagta
     2701 tattgaatat tatcattaat agtgcaccac caacagctgc tacggatctg tttacctttt
     2761 ctgtaataat agctgccata actattaaaa atatacatat agctattatt tgttgaaatt
     2821 ccataatata agtacctcca tttgtattat tttgtgccta atgctagtac tatatatagt
     2881 attacttttt tattgttttt tcaatggatg tatgaaagta tattatttaa caaagttttt
     2941 ggaaaattga cagaatttaa agaaaatttt tgttatttaa ataaatttta aaattattcc
     3001 attgataata atattaaacg acaaacgaaa aaatataccc tttaaactag aatttattgt
     3061 atctaataac aattataaaa tattaaatat atctatatta taaaaacaat aaaaaaaggt
     3121 ttgatttgaa tataaaactg caatatatta aaaaaattat tcattttaaa ccttgtaatt
     3181 tgattttata aatattataa ttaaattaat gaatttttaa caaggaatta ttcatttttt
     3241 tgattttaag atttattgag aaaacaaaac taaatagggg ggtaattaat ataatggatt
     3301 ttttcaatgg tattttaacg agtattaaca atgcagtagt atatgtaaac aattacttat
     3361 ggtcgtatat cttaattata atgttagttg taattggtat ttactttact attaaaacaa
     3421 attttgtaca attcagatat tttatagaaa tgtttagact tctgggtgat ggtacagcga
     3481 ataaaaatgc aaaaaaggaa ggaaaaatct cttcatttca agcattttgt ataagtacat
     3541 cttcaagagt tggaactgga aatatagctg gaatagcaat tgcagtggta gcaggaggac
     3601 ctggtgctgt attttggatg tggcttatag cattaattgg ttctgcatca agctttgttg
     3661 agagtacatt agctcagatt tacaaagtga aagatggtca agcttttaga ggtggaccag
     3721 cgtattatat ggaacaaggt ctaaataaaa aatggatggg tattttattc tcaatactta
     3781 taactatatg ttatggtttt gtatttaatg cagttcaagc aaatactgtt tcacttgcat
     3841 ttaataatgc atttggaata agtaaaatgg ctatgggaat aggtcttgga attttaactg
     3901 caatagttat ttttggtgga gtgcatagag ttgcaaaagt ttctgaaata atagttcctg
     3961 tatttgcagg attatatata ttagttgctt tggctatagt tgtaatgaat ataacagaga
     4021 taccaagtgt tatagcattg atagttgaaa gtgcatttga ttttaaagga atggcgattg
     4081 gtacatttat gggagttgta atgactggag tgaagagagg tctattttct aatgaagcag
     4141 gtatgggtag tgctcctaat gctgctgcaa cagcacatgt aactcatcct gtaaaacaag
     4201 gtttaattca atctttaggt gtttttacag atactataat aatatgtagt tgtacagcat
     4261 tcatggtatt attatattca ggttatagta gtagtggagc aactggaatt gaattagctc
     4321 aagaagcttt aactcatcat ataggaccag ttggaaatat atttatagca gtttgtatat
     4381 tcttgtttgc atttagttct atagttggta attactatta tggtgaatca aatatggagt
     4441 ttatgagtgg aagtaagact aaacttaatg tatttagagt ttttgttgta ggtatggttt
     4501 tatttggttc acttactcaa gtggatgtag tttggaatct tgcagactta tttatggcat
     4561 tgatggcgat aataaactta atagcaatag cacttttagg aaaatatgca tttattgctt
     4621 taaaagatta tacaagtcaa aaaaaatcag gaataaaaga ccctgtgttt attgcagatg
     4681 aaatagaagg cctagaaaag gtatcagagt ggcataggga attatctgag gaacatttag
     4741 gataaaaaat agtataaaat tataaaacca ttttgaaaga ctagagtatg tatatacttt
     4801 atatgaatat aatattgaat taatttatat tactggaatt tttaattcca gtaatatttt
     4861 tttattatat aatttagctt tattaaaagt ttttattatt aaaaatagct tatttatcgt
     4921 aaagtgaatg atattgggtt ttaattatta tgaatgttag tatttaattt aaagacttaa
     4981 ataaatttgc catttctagc gcattcatag cacaatcata acctttattt ccagcttttg
     5041 tacctgctct ttcaatagct tgttcaatat tttctgtagt tacgattcca aatataacag
     5101 gtaattcact gtctaaagat actttggcta tccctttaga gacttcacta catacataat
     5161 cataatgaga agtagcccct cttataacac agcctaaaca gattatagca tcatacttac
     5221 cacttttagc cattttttga gcaacaagag gaatctcaaa agctccaggt acccaaacta
     5281 tttctatatt ttctggagaa acgtcatgtc ttaaaagaca gtcttcagct cctgataaaa
     5341 gttttgatgt tataaattca ttaaaacgag aatttattat acctattttt aaatcttttc
     5401 caataagttt tccttcatat atcattttta cttcctccaa ttttatttat gttatagatt
     5461 tatatttata aatatcttta atttaattat aaaaaactac ttagtagaaa aatatcatca
     5521 tataatcatt tatataatat ctaaaagatg tcccatttta tcttttttag tttttaaata
     5581 tagcttatta aagtcattaa tttcttcatt aatagggatt ctattttcga tttttattcc
     5641 atatttctct aattgattta ttttatctgg gttattactt aaaagattta tactttttat
     5701 gtttaaattt tttaatattt gagcagccat atagaaatct ctcatatctt cttcaaatcc
     5761 aagcattaaa tttgcttcaa cagtatcgta accttcttct tgaagtttat aagccttaat
     5821 tttatttaaa agaccaatac ctctaccttc ttgcttcata taaattaaaa ctccacttcc
     5881 attttccaca attttcttca tagaagattc tagctgtaat ccacaatcac attttagaga
     5941 gtggaacaca tctcctgtta aacattcaga atgaagtcta actagagtat tttctatatt
     6001 tatatttcca taagtaaggg caatgtgttc ttcatttgaa taagtatctc tatatcctat
     6061 gatttcaaaa tttccatatt ttgtcggaag gaaagcacta gatacttttt ctatagtagt
     6121 ttcatttatt ttgcggtatt ctataaggtc ttttatagtg atgattttta aattatgctt
     6181 ttctttgaaa atcattaaat catcaactct tgccattgta ccatcatctt tcattatttc
     6241 acagataaga ccaatctctt taaaacctgc aagtctagca aggtctactg aggcttctgt
     6301 atgtccatcc ctgacaagaa ccccaccagt ttttgctatt aaaggaaaga tgtgaccagg
     6361 cgttttgaaa tctttacttg tagaattttc atcacagaat aattttacag tttcagctct
     6421 ttcatatgca gatataccag ttagagtatc tgctccatct atagtaacag taaaggctgt
     6481 ttgaaatgta tctgtatttt ctctaaccat aagaggtata ttaagacttt taaatttttt
     6541 ttcagtggca ggtagacaaa ttagacctct accatatgta gccataaaat ttattgattc
     6601 aggagtagcc atttcagctg ccatcaataa atctccttca ttctctctac tttcatcatc
     6661 aaccacgatt accattttcc cattttttat atcttcaata gcttcttcaa tagtattaaa
     6721 catgtaattc actcctttat tatttttaaa aaccattttt aaataaaaaa tctttatcta
     6781 tatttgattt atggttatta ctattagaat ttaattcctt gtagttatta gccataaaat
     6841 tattaacata ctttccaatt acatcgcttt ctaaattaac gaaatctcca acattctttg
     6901 tcaagagaat agtgttaatt ttagtatgag gtataataga aaccttaaat atttcatcat
     6961 ctaaatatga tatagttaaa cttacaccat caatagcaac agaacctttg agtatcattg
     7021 aggataaaag ttttttatca ggtcgtatac ttactaaaac tgcattttca tctttttcaa
     7081 atttgcagat agttccttta ccatcaacat gacctgttac tatatgacca ccaaatcttg
     7141 aagaaagact catagctctt tcaagattta ccttgtcatt tgcatttaat aaacctaaat
     7201 ttgtggaacg aattgtctcc atcataacat cggctgtaaa ttcatttttt ccgaggtttg
     7261 aaacagttag acaaactcca tttacggcta tactatctcc aagatttgtg ccatctaata
     7321 ttttatttga tagtatggtt acttttccac tcttaccatt agcagtaatt tttctaagta
     7381 ttccaacttc ttctactatg ccagtaaaca tcctaacctc ctatatgaat gtattaacaa
     7441 aaatttaatt taaaacatct gattctataa gaatatcgcc atctatcaaa gttacattat
     7501 tgattgttaa ctgtgttgaa tctgacaaat gatttattcc ttctccagaa acgaatgtag
     7561 gtgcatcttt accacctata atcttgggtg atagaaataa tttgacctta tcaactaact
     7621 tatttctaaa gagactgtca ttgagagtag caccaccttc aactagaaca gaatcaatgt
     7681 ttaactctcc taatttatac attacaaact ccaaatctac taagttgttt tttgaaggag
     7741 aaattattgt ttcaacatta ttttctttta acatagataa ttttatactt tctttgcaac
     7801 atgtgaaaac tatagtttta ctggttttat ctttaaccaa attactatta agtggcaact
     7861 ttaggtgtgt atctattact attcttgtag gatgagacac tttttcttga ttgagcctac
     7921 aagttagtaa aggattatcg ttcaaaactg tatttatgcc aaccattata ctttggtatt
     7981 tatttctgta tttatgagtt agataccttg atgattcatt agatatccat tttgattcta
     8041 gtgattttgt agcaatttta ccatctagac ttactgctga ttttacaata caaagaggtc
     8101 ttttgttttt tatatgataa aaaaatgcct catttaaatc tcttgcttca tcttctaata
     8161 ttcccacagt aacatcaata ttgttatctt ttaatttttt tactccatta ccacaaacaa
     8221 gtggatttgg gtctaatgta gatattacaa ctctcttaat cttattttgt ataattttat
     8281 ctacacaagg aggcgttttc ccataatgag aacatggttc taagtttaca taaagagtag
     8341 agtcttttag actttgttta gcactgttta tagcatttac ttcagcatga ttagagccaa
     8401 atttctcatg atatcctttg cctattatat tactagcttt aacaattaca cacccaacta
     8461 atggatttgg atttacaaag ccttctccat ttttagccaa ttcaatagct tttttcatat
     8521 aataaatatc tttttctttt tgattcacaa aattcacaat atgtacacct cctgtttaat
     8581 tggataattt ttaaacaaaa aaccctgaga attaactcag ggtttagaat aatctacaat
     8641 aaatgtttaa taaatgtaga acaaaattgc tctacaaaaa cactcattaa atatcttctt
     8701 ccatccagac tttactgtcg gctttggaat ttaaccaaat caaccaagtt ttaccttggc
     8761 tcgcgggcta taccgccgat tgggaatttc accctacccc gaagattaag tattaaattt
     8821 atatttagaa tgtatcataa attattttac tttgcaaact atttttttaa aaaatatatg
     8881 ttaatatata atatagaatg tctataaact ttgaaatacc acgattgtga atataaaaat
     8941 aaaaatttga acataatcag gaaagaagtc taattaattt aaattaggag agtaatatat
     9001 gaataaaaaa cttttagaaa tactatcaaa atactatgga tatacaagtt ttagaaaagg
     9061 tcaagaatct ataataaact ctatcttaag cgaaaaagat gtgttagcta ttatgccaac
     9121 aggaggggga aaatcaatat gttatcaaat tccagctttg attttagatg gaatgacaat
     9181 agtaatttct ccacttatat cacttatgaa agaccaagta gatgccttaa agactatggg
     9241 aattagtgct gcttttataa atagttcatt aagtagcaaa gaatttaatg aaatattaaa
     9301 taatattaga aaaaataatt ataaaatact ctatattgct ccagagagat tagatgcaca
     9361 agagtttatt gagctaataa ataataacaa tgtaagtcaa gttgcgatag atgaagcaca
     9421 ttgtgtatct caatgggggc atgattttag gttgagttat agaaggattt ctgattttat
     9481 aaaaaatttg cctaaaagac ctatagttac agcatttact gcaacagctt cagaagaggt
     9541 aagaactgat ataataaatt tactgtgttt agaaaatcca gattattata taactggatt
     9601 tgatagagag aatttatcaa taaatatagt aaaatcatct agtaaaaata agtatatttt
     9661 agattatatt caaaatcata aaaatgagtc tggaattata tatgttgcaa ctagaaagga
     9721 agttgaaaat atatataatg gtttatctaa gagaaatata tctgtgtcaa aatatcatgc
     9781 aggtttatct aaaaatgaaa gaagtaaaaa tcaagaagat tttataaatg ataatgtgga
     9841 tataatggta gcaacaaatg catttggaat gggtattgat aaacctaata taagatgggt
     9901 tatacattac aatatgcctc aaagtataga aaactactat caagaaatag gtagagctgg
     9961 tagggatgga gaaaaaagtg agtgtgtact tttattttct ccaggagatg tacatataca
    10021 aaaatattta attgatattg gagttgaaaa tcctgaaaga aaattatttc agcataagaa
    10081 gcttcaatac atgattgact tagtttacag taattcatgc tatagaaaga caatattgag
    10141 ttattttgga gaaaattata tagacaattg caataattgt agcaattgtt tagtagaggg
    10201 tgaaatagta gataaaacta tagatgcaca aaaagtcatt tcatgtgtag ctagaatgaa
    10261 aagaagttat ggtgttacta ctattgtaga tgtgcttaga ggttctaaga ataaaaagat
    10321 tttacagctt ggattaaata ctttgtctac atataacatt atgagagatt attctagtga
    10381 agacttaaag aattttgtaa atacattggt ttctcatggt tttttagatt tagttgagac
    10441 tctaggcaat ggaaatagag gtagttttcc tactataaga ttaaatgaga tgtctatgaa
    10501 agttttaaaa ggtgaagtga aagtagagtt taaagaaatt gttatgacaa aatcattgga
    10561 agtagaggat gagttatata atgctttaag agaacttaga cattcaattg caagtgaaga
    10621 aaggatagct ccatatatgg tatttggaga tggtacactt agagccatgt ctagtagtta
    10681 tccaattaat aaagaagaaa tgctagatat ctctggagta ggagaaatta aatatcaaaa
    10741 atatggtaga gattttgaag cagtaattaa agaatatgtc gaaaaaaata atataaataa
    10801 aaaaatagat gaaaaaaatg tcgattcaaa gaacgttaat agtgatttct tagaggttaa
    10861 tacagataaa gttttatatg aaaaactgtt agtcattaga gaagaatatg caacaaagga
    10921 aaaattacct gcatatatga ttttagccaa taaggctttg aaagaaataa gtggaagata
    10981 tccacttgat gaagaacaat taaaagatat ttctggcata ggtggagtaa aatttgaaaa
    11041 atatggagct agtattttgg cagaggttaa taaatatgta aaagaaaata atattaatgt
    11101 tatttgggag aaaaagggca aaaggaagtt aatattagat ggagaaagta gaaaaaataa
    11161 tgaaatagca ttagatttgc taaatcaagg aaaagatata caaagtatca gtcaagaaat
    11221 agaagtttca ctgtcaacca ttattggata tgtgcatgac tatgtaaagg atggacatag
    11281 tatatcattt gaattaggac tagaaaattt ttataatgaa gataaaaaga agatgatttt
    11341 agaagtttgc gaaaaagttg gctatgataa tttaaataac ataaaaagaa aacttccaga
    11401 ttcaataaag tatgaaaata taagagctgt gatacttgac tcttatcttg aaaacttaaa
    11461 aaataatatt atagcagagt aatttttaac ttaataaaaa ataaaatttg tattattgtg
    11521 ttgaactaat aattttgctg tgttatactg aatttgtaaa aataatgaaa aattagagct
    11581 aggggcgcta gaattggcta gctgagagat aaagccgtaa cttacaaacc ctaataacct
    11641 gatcaagata atgcttgcga agggaagctt tttaatataa ttttattata taaataggca
    11701 aattattcaa tgttaggaga aatttgtcta agtgtatata cgattataag agtgctttcc
    11761 aaacgaggaa aagcactttt tttattttca aaaactataa ctttaatctt ggaggattgt
    11821 aatatgaact atacaacaca aatggatgct gctagaaaag gcataataac taaagaaatg
    11881 gaaatcgttt ctcaaaaaga acaggtagat gtaaatgaat taagagaatt aatagcaaat
    11941 ggacaagttg ttataccagc taataaaaat cataaatctt taagtgcaga aggtgtaggt
    12001 aaaaatttaa gaacaaaaat aaatgtaaat ctaggaattt caagagattg taaggatata
    12061 gaaaaagaac ttgaaaaagt tagagtagca atagacatga aagctgaagc tataatggat
    12121 ttaagtaatt atggcaaaac tagagaattt agagaaaagg ttgttgaaat gtcacctgca
    12181 atgataggtt cagtacctat gtatgatgca gtaggatatt tggaaaaaga attaaaagat
    12241 ataacagaag aagagttttt aaatgtaata agacaacatg ctattgatgg agtagacttt
    12301 attactatac atgcaggtct tacaagaagt gtttgccaaa aaataaaaaa tcatgagaga
    12361 ttgactcata tagtatctcg tggaggttct ttattgtttg catggatgga attaaataat
    12421 aaggaaaatc ctatttatac taattttgat aaaatattgg atatatgtga agaatatgat
    12481 gttacattga gtttaggaga tgcttgtaga ccaggttgta taaaagattc tactgatggt
    12541 gttcaaattc aagaacttgt agtattaggt gaacttacta aaagagcatg ggaaagaaat
    12601 gtacaagtaa tgatagaagg tcctggacac atggctattg atgaaataga agcaaatgta
    12661 gtattagaaa agagattatg ccatggagca cctttttatg ttcttggacc attggttaca
    12721 gatatagcac caggttatga ccatataaca agtgcaatag gaggagcttt agcatgtgcc
    12781 aaaggagtag attttttatg ttatgtaact ccagcagaac atttaagatt gcctaattta
    12841 gatgatatga aagagggtat aatagctgca aagatagctg ctcatgcagg agatatagct
    12901 aaaaatgtta aaggtgctcg tgaatgggat aataagatga gtaaagctag agctgattta
    12961 gactggtgtg agatgtttag acttgcaata gaccctgaaa aagctaagag atacagagat
    13021 gaatcaactc ctactcatga ggatagttgt actatgtgtg gaaaaatgtg ctcaatgaga
    13081 acagttaaga aaatattaaa caatgaggaa cttaatctaa tataattttt ggctagtatg
    13141 actggaataa aaataagctg aaaagaggag gtgatttagt gaaggttaat gggaaggaaa
    13201 ttgaatttga aaaagatttg actgttatag atttattaaa taaatataat ttaaaaagtg
    13261 atagagttgt agttgaagtg aatttagaga taattgaaga atctaactac aatacatatg
    13321 tattaaaaga cgaggacatt gtagagctta ttagctttat tggaggtggt taaaataaaa
    13381 atatttgtaa atgaattatt aacagatgtt gaggaaaata caactgctta taaagttaga
    13441 gatagctata acaataaatg tgatgtattg gtgttaaatg gatatccaat taaaacagat
    13501 atgacccttt gtgaaaatga taaattaaca cttatacaaa aaggagttaa gccatcactt
    13561 gatgaattgg aaagcttgtt gattgctaga catactccaa atatacataa taaattaaaa
    13621 aaagggaaag ttgctatatt gggattgggg ggacttggtt ccaatatagc tatctcttta
    13681 gctagaatag gagtgggaga acttttactt atagattatg atgttgtaga accatctaat
    13741 ttaaatagac agcagtattt tatagatgat ataggaaaac taaaaactga tgccatgata
    13801 gaaaatataa aaaaaataaa tccatttata aaattaaata aacgagatat ttttttaaac
    13861 aagaacaata tggatactat taaggattca gatttaataa ttgaggcctt tgatgatgct
    13921 tcatgtaaag cacaggtatg taattatgta ttaataaatt taaaagataa atatttaata
    13981 gcatcatctg gtatggcagg ttactatgat tcaaatataa taactactaa gaagataaaa
    14041 gacaggtttt atatatgtgg ggattttgtg aatgaagcta aatttggtga agggcttatg
    14101 gctccaaggg ttgccatctg cgcaaatcat atggcaaatt tggcaacgca aatacttata
    14161 gatatgtaaa tattaaaatt agatataaaa aagattgaat attaaaaaaa tttaacgtgg
    14221 agggaaataa tatggataaa ttggtacttg gtggacatga atttaatagc agattattgg
    14281 ttggaacagg taaatatggt tcaaataata tattgcctga ggtaataaaa gaaagtggaa
    14341 gtgaaataat aacaatggca cttagaagag tggatttaga caataaacaa gaaaacatat
    14401 taacgtatat tccaaaagag atgacaatac ttccaaatac atcaggagct actaatgcag
    14461 aagaagctgt aagaatagct agaatatcta gaaaaatggg atgtggagat tttataaaaa
    14521 tagaagtaat atcagatact agatatttac ttcctgataa tgaagaaact ataaaagcta
    14581 caaagatact tgctgatgag gggtttatag ttcttccata tatgacacca gacctttatg
    14641 ctggaagaag acttatagaa gctaatgcag cggcagttat gccacttgga gctccaattg
    14701 gttcaaatag aggtcttcaa atgaaagaaa tgataagaat aatgatagat gagttggata
    14761 taccaataat agtagatgca ggtattggaa aaccatctca agctatggag gctatggaaa
    14821 tgggagctga tgcagtactt gtcaacactg ctatagcttc agcaggagac cctgtacaaa
    14881 tggctagagc atttaaatta gctgtagaag gtggaagaga agcatatatt gcaaaaacag
    14941 gaaatgtgtc agaatttgca aatgcatctt ctcctttaac aggtttttta ggcaatttat
    15001 agaaagtggt gattggtgat gagtttttat gatgtaatag aaaaatatag ggattttgat
    15061 tttgatggat atttaaataa tgttactgat aatgatgttt taagaagttt atcaaaagac
    15121 aagcttgaag attttgatat attaaatttg ctttctaaaa ctgcagtaaa acatttagaa
    15181 gatatggcac aaaaagcaca taaattaagt gttcaatact ttggtaaaac agtatgttta
    15241 tatacaccaa tgtatatagc aaattattgt gttaatcaat gtgtttattg tagctataat
    15301 ataaagagtg gaataaaaag aaaaaaatta actatggatg aaattagaga agagggagaa
    15361 gtaatttcaa aagaaggttt caaacatcta cttgttttga ctggagaaag tagttttcac
    15421 tcaagtgtag aatatatagg tgaggctata gaaatattaa gagagaaatt tccctctata
    15481 ggtatagaag tttatccaat ggaagtagaa gaatataagt acattgtaga taaaggtgtt
    15541 gaaggattaa cagtatatca agaaacttat gacgaggaaa tatataagag agtacatata
    15601 aaaggcccaa aatcaaatta taaatttaga ctagatgctc ctgaaagagg ggcaaaagca
    15661 ggtatgagga ctttatcaat aggtgcactt ttagggctaa atgattttag aaaagaaact
    15721 ttttttacaa tattgcatgg gaaatactta aaaacgaaat atcctcatat tgagctttca
    15781 tattcaacac caagaatgag accttttaaa gggtgttttg aggaattggt agatataagt
    15841 gatactgatt tagttcaagc tatggtatgt atgagattat ttgaccctca tgcagctata
    15901 aatatatcca ctagagaaaa tttagaaatg agaagtcata taataccttt aggagtaact
    15961 aaattgagtg caggtgtatc tactgatgta ggaggtcatt ctcaagatga acatgatact
    16021 gctcaattta agataaatga tgaaagtact gttaaagatg tggagaaaat gctaaactca
    16081 attggttatc agcatgtgtt taaagattgg gaaagatttt aaatgtatct gataacaaac
    16141 agaaaattat gtagtgagga acgatattta gaagttatta aagagtctat attgagtggt
    16201 gtagaaaata taataataag agaaaaggat ttagaatatc aagagctaag aaaattatat
    16261 atgaaaataa aaactaaaat caattgtata gattttcaag agcaaatatc agatgaaagt
    16321 ttgaaaacta atataaatca aaaagagttt agaaataaat tcaaggttaa ttttataata
    16381 aatagtaaca ttgaattttt tgaaaaagtg gattgtcaag gtatccatct tccttttaaa
    16441 ttattcttga atttaattga aaataagtat aattttaatg aaaataaaat actaggatta
    16501 tctttacata aagtagagga agtagattat ttagaaaaat taataagaaa tcaaaatata
    16561 aaaatagatt acataacatt atcacatata tatgaaacta aatgtaaaga aggattaaat
    16621 ccaaaaggaa ttgagctttt aaaagaagct aaaaagatta ctgatattaa aataattgca
    16681 cttgggggaa ttttaccatc taatgtcaag gaaacattaa aatactgtga tgattttgct
    16741 ataatgtcaa ctataatgag gtctaaagat attaaaaaaa ccatatcaaa ttataatgaa
    16801 aagttgaatt agtaaatata gtatattgaa tttttaactt aaataaaaat aaaaaagtta
    16861 tcttttaaat tttaaaaggt agctttttta ttttatatct ttgctaataa aaaattgtaa
    16921 aaagggaaaa ctttagtcag agagattaaa caatatccta gaaagattta aaatttagta
    16981 aagtatggag gacattataa tgactgacag taaaaagaaa cgatttatta taccacacac
    17041 ttttacaata atatttttac taatagtctt gatggcaata ttgacttgga tagtaccaag
    17101 tggtgaattt agtagagaag atgtagaagg tagaatggta gttgtaccag ggacttatca
    17161 aagtgtagaa agtaatcctc aaggatttgc agatgttttt aaagcaccaa tagaaggctt
    17221 tatcgattca gctgaagttg taggttttgt tttaatagtt ggaggtgctt ttggtattgt
    17281 aaataggact ggagctatag aagcaggaat agcttcggtt atctctaagt ttaagggaaa
    17341 agaacttctt ataattccaa tatcaatgat tttatttgga cttggaggta caacatttgg
    17401 gatggcagaa gagacattac cattttatat gatatttgtt ccactcatga catccttggg
    17461 atatgattct ctaacagcaa tagcaattgt atttatagga gcagcagcag gagcagccgc
    17521 ttcaacaata aatccatttt cagttggtat tgcacaggca ctagctcaac ttgctcctgg
    17581 ttcaggagta acttacagag tagttatttt tatagcatat atactaataa gtatagcatt
    17641 tgttatgtac tatgctaaga aggtaaagga aaatcctgaa agttcaatag tatatgaaat
    17701 agataaaaat aataaatcaa tacttaatca aacttcaaca gaaataaaag aatttacttt
    17761 taaagaagta tctgttcttg ctatatttgg aatagggatg gcaataatga tttggggtgt
    17821 tttaagtcaa ggatggtaca ttccagaaat atcaatgtgt tttgctgcaa taggtgtgct
    17881 ttctggattg gttggtagat tatctcaaat tgaaatctca gaaagtttta tagatggagc
    17941 aaaagattta gcatctgcag ctcttgctat agctttggct cgtggaatcg taataattgc
    18001 tcaaaatgga tttataatag atactatatt aaattccgca gcagcctttc taggtgggct
    18061 tccaaaggtt atatttgtat atttatcatt tttcttggaa gctgcattgg catttttaat
    18121 tccatcatct tcaggtcttg cttcacttac agtacctgta cttgctcctc ttggtgattt
    18181 ggttaatata tcggctcaag tgatagtaac cgcttatcaa tttggtgtgg gtcttacaaa
    18241 ctttataact ccaacttcag gagtactgat gggagcactt gcagtagccc atattccatt
    18301 ttcaaaatgg gtaagattta taatgcctat cttgttgatt ttgacagctg tatctctagt
    18361 ctttttaaca ataggtattt atatagggtt ttaattaagt gtagttttat ttaagatatt
    18421 tagtaatggt ataaaatgat aatatgtttt caataaattg ccactaattt aattagtggt
    18481 aatttgtttt ttgtatttaa aaataaatgt atttacttgt attttgaatt aatatctact
    18541 tagacattgt tttaattgtt atatttttat ttaattaagc attgatttat cattttttat
    18601 atttcatata acaatgcaaa aaataatttt tatatagtaa atgacaaaag tttacaataa
    18661 atgtatattt tcttactcat tccttaaaat tactcattta gtttaataac tctcatttca
    18721 tatgtaaagt tggtaaaaaa attaaaatta aaagtaaaaa actaaataaa ttaatgttaa
    18781 tttgaaatat atatgaaggt ttttggagaa tacttactta tgataggaaa aaatgttaaa
    18841 ataatagtgt aatcaaatat aaaggactga aataattaac agttattaaa tattaggagt
    18901 gtaaaaattt atgggattaa aagaatttga ttttattgaa gagtctattg atatgttgag
    18961 gacaatgtca ccaacattag aaactatatc agatgaaata gaagaatatt ttgaaaatat
    19021 cttggatgaa aagaatcaag aatatataaa tgtaacctct agaataaaat cagaatcaag
    19081 tttaagagaa aagataatta gaaatagata tttaaaaaaa tatggagaag caagtaatct
    19141 tattcataat gtttctgact taataggcct aagaattgag tgtagattta ttgaggatga
    19201 aaacaaaata tatagacttt tgagaagata ttttaataaa acagatgaca aaataaacta
    19261 ttataataaa gagaataaga atattaaatt aaaactatct gagaggcaac ccaataagca
    19321 aaaaaatgga tttgaaatat ataaaataga tggagttttt gcgtatttag atagagaagt
    19381 aaaatttgaa cttcaaatta aatctcttgt aaatgtgttt tggagtgaaa tagaacataa
    19441 aataatttat aaaaacaata catatttact tgctgataaa tttataaagg atatgatgga
    19501 ctctataaag aataatctta ctatgataga taatcagctt ttaagtattt ataaaaactt
    19561 tcattcaggg aagtctttta atatgaaagt aagtaaaaaa gaaattgaaa aattatttgc
    19621 gaaactagtc tatgacgctt tttctgaaaa gatgaataaa agtataggtt ttgttgtaga
    19681 ttttaaaaaa ccatgtgaga ctatattgaa ctattctttt aataaacaag atataagtga
    19741 tgaagtttta ggtaatttta tgcttgatga gtttgcaaga ttaaatgaaa ttgtaaacaa
    19801 ggatatagat tttaatgagc aaatagaatt tgaaagagaa ccaacatttg atgataagtt
    19861 ttgtaaagac ctaggaaatc attttagaag tagattaaac acagaatttc catggaacct
    19921 attttttaga atattatttg aaatagagcc atataacaat accaaagact ttgaaaactt
    19981 tgtagagttt ataaaagaaa atgtgctact agaagaaaat agggagcaat tattatgcca
    20041 atttgaagag gatagtaagc ttataattga agatatatat gattgtatat ttaatagtat
    20101 aagtgagatt gatagtgttg aaattttata taactataat cttgaaaaaa taaactttac
    20161 atcaagtgaa atcataaatt gtatatgtag agaatatgag tgttatggtg aatacttaga
    20221 agaaaaagaa aagcttatga atacatttaa agaaaagatt attcaaatct ttgagtaata
    20281 ggtcaatgct gaagtggcgc taatgcgcca tttttcaaat atatatgaat taagtaaaat
    20341 tattttggag gaggcgattt gtatagagaa aacaggtgta attttggcag ggggaaaaaa
    20401 ttctagaatg ggtagagata aagccttttt agagttacat gataagcttt ttattgaaat
    20461 tgcaatagaa gcatttaaaa actttgatga gttgataata atatctaaca atgaagaatt
    20521 atattctaaa tacgatatta aagtttataa tgatatagta aaagatgttg gtccaattgg
    20581 aggaatatat actgctcttg aatatgcaaa gtatgatata gttacaattg catgtgatat
    20641 gccatatcta aataatcaaa tagtcgaaag aatagctaat aaaatggatg ataaaagtgt
    20701 gatttcagtg acaaatggaa agttacaacc attgtgttct ggatataaaa agagtataat
    20761 aaataaagta tctttatgta taagggaaaa tgatttaaag ctcagaagct ttatagataa
    20821 aatagataaa agttatatat actttgatga agaagattta tttttaaatg taaacactgt
    20881 tgatgagtat aaaaaattaa ctaaagttta attagctatg gttgtaaaat agagtaggat
    20941 ttatatggag gagagtttat gaatttactt aaatgcgatt cttgggcagt aatattaaat
    21001 aatagcgata aagaaagtaa agcatataaa atattagatg aacttaaaag gaatatgtac
    21061 aaagttgttg ctatagatga agagaaaaaa cctattgaag gaatagatgt gtatgaatgt
    21121 ttaaaagatg ttccacacaa tattgatgtt gtagctatta ttgataaaca atctaagatg
    21181 gatgtgattt tagaagaagt agagcttttg gatatacaaa atatgtggtt tgaaaaagga
    21241 agctttagtg aaatgatgat taaaaagact aaagatttaa aattaaatat agaatataat
    21301 ttaagtttat atgatgagtt gactagataa aattatattt cttaataata aaaatataat
    21361 tagttaataa aacatattat tttatagtat agattggtta ataaaagtac agattggtta
    21421 atgaaacatt aaattacaat atttgttaaa aaaattgtga gttacatgat ataatgtatt
    21481 aaataggata ctaaggagtt gatatattat gttaatagta actacagaaa aagtagaagg
    21541 taaaaagata tcaaaggttt taggattagt gagaggaagt acaataagag caaaacatgt
    21601 tggaaaagat ataggagcaa gttttaagaa tcttgttgga ggagaactta ctggatataa
    21661 tgaaatgctc actgaagcaa gacaaattgc cataggtaga atggttgaag atgcagaagc
    21721 taaaggtgca aacgcagtaa tagcatttag actgtcttca gcttcagtta tgcaaggagc
    21781 agcagaaatg cttgcttatg gaacagcagt tgttttagaa gatgataata gtattcttga
    21841 aaaataaaat ataaatagtt aaaaagaggc atcttttaaa aattttatgg atgccttttt
    21901 ttatttgaaa aaactaattt gagaagaaat tttaactatt atgacaataa aattagggtg
    21961 atattatata aataagtaaa attatattat ataatgtatg gtatagactt taaaaatttt
    22021 atttgtagag aaaaatatgg acaatgttat aataattata gaaattaaaa gtacttacat
    22081 tcattatgta tgtgacatgt ataattagtt ttatatttaa tacagttttg aagtaatatc
    22141 ttcattttga gaacgttgga ataataaaaa atgtattatt ataggtgata taaataaaaa
    22201 ataaatataa tatgttatat tgctgataga aaagagaata aaagaaaata ggttttatta
    22261 aaagagattt ttatttatag gacttaatca atgtatagag gggtgagaac atatgtttaa
    22321 tgttgcaatt ataacactta gtgataaagg atatgaagga aaaagagaag atataacagg
    22381 aaaaaagtta actgaatttg ttgaaaatac aggagtttat aaagttactg aatatgtact
    22441 tataaaagat gataaggaaa tgctaaagga aaatattata agactttgta atagtaataa
    22501 aatagattta attcttacaa atggaggtac tggtttttca aaaagagata taaccccaga
    22561 agcaactaag gaagtgttgg aaaaagaaat accaggtctg agcgaatata tgagaatgaa
    22621 atccacagaa attactaaaa aggccatact aagtaggggt gtgagtggaa taagaaataa
    22681 ttctataata ataaatttac ctggaagtcc taaaggtgca gttgaaaatc taagttttat
    22741 aattgatgtt ttagaccatg gcatagaagt tttaagagga gaagctacag aatgtgccac
    22801 taaaaaaagt gaatagtata taatctagat agataaaaaa tagacaaaga ggtagataaa
    22861 gtatgattga taagtatgga agaaaaatag actaccttcg tatttcactt acagataagt
    22921 gtaatttaag gtgtgtctat tgtatgccac cagatgtaaa atttgataaa aattatataa
    22981 atgaaaatct aagctttgaa gactacaagt ttattataaa ggctatggct gaacaaggta
    23041 taacaaaaat tagatttact ggaggagaac ctcttttata tcctaaatta aatgagctta
    23101 taaaatttac aaaagaagaa tgtggaataa ataacatagg aatgaccact aatgctattg
    23161 gtctttctga tagaattttt gagcttgaaa aatgtggctt aaataaagtt aatataagtt
    23221 tagattcttt gaaggaatat aagtataaat ccataacaag aggaggaaac ttaaaagatg
    23281 tcttaaaatc gattgaatct tgtttaaatt taggtataaa agtaaagtta aattgcgttg
    23341 ctataagtgg gtttaatgat gatgagctta aagattttat gtttttaaca gttaaatcac
    23401 caattgatgt aagatttata gagcttatgc cattaggtga ggctaataga gtttataaga
    23461 agggttattt taatgtaaaa gaaatgattg aaaaaatata tggtttatat aaagtcaaaa
    23521 atgatgaaaa aagtacagca gaatattata tgtttaaagg agctaaagga agaataggaa
    23581 ttataactcc attaagctgt tcattttgta acacttgtaa tcgtattaga cttacttcta
    23641 gtggtgcaat aaaattatgt ctacattcaa aagaagaaat agatataaaa ccatttttac
    23701 ataaaccatt gttatttaga gaatctatga aagatataat aaaacaaaaa ccagaaaaac
    23761 atcatttaat agaaaataaa tgtagtgata caaataggcg tatgtatgaa attggaggat
    23821 aagtaatgtt aacacatata aatgaaaaag ggtatgccaa gatggtggac ataagtgaaa
    23881 aagatgatac taaaagaacg gccgtagcta cagctacaat tactcttagt aaggaagcat
    23941 tagaaaaagt aaaaaatggt cagataaaaa aaggagacgt attgtctgta gctcaagttg
    24001 caggtataat gggagctaaa aatacatctt caaacatacc aatgtgccat cagattaaat
    24061 tagttggatg tgatttagaa tttgatgtag atgattctat gtctgctatt aagatatacg
    24121 caacctgtaa atctcttggg aaaactggag tagaaatgga agctttaaat gcatgttctt
    24181 tagcagcact tacaatttat gatatgtgta aagcagttga taaaaaaatg gttataagta
    24241 atatccatct tgtggagaag actggtggaa aatcaggaga ttttaagttc gagtaaatta
    24301 ataatatatt gggttaaaat ttggaggaaa tatggcgaag gtagtgtcaa taaatataag
    24361 tgagaaaaag ggtgtaatta aagttcctgt acaatgtgca gagctaaaaa taaatcatgg
    24421 gatagttgga gatgcacatg caggaaactg gcatcgtcag ataagcttac ttggaaaaga
    24481 aagcatagat aaaatgaaaa tgaaaggctt tgaccaatta aaatatggag attttgctga
    24541 aaatgtaact acagaaggga tagaagtttt ttccctgcct gttggtacaa agttaagaat
    24601 agggcaatgt gaagttgaag taactcaaat agggaaaaaa tgtcacaatg gatgtgaaat
    24661 taagaaattg acaggtgact gtgtgatgcc acgagaagga atttttgtaa aggttctaaa
    24721 agaaggaatt ataaatgtga atgattcaat agatgtaatc taatgaagtc actattaaaa
    24781 tataattata agtaaatact attaagattt aaatatatat tttaagagag aattaatttt
    24841 aataaaatat tttagttata aattggaata ttggttata
//
LOCUS       DAYATN010000047        24794 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.047, whole genome shotgun sequence.
ACCESSION   DAYATN010000047 DAYATN010000000
VERSION     DAYATN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.047"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            43..381
                     /locus_tag="ACGI8A_001815"
     CDS             43..381
                     /locus_tag="ACGI8A_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HFH0179813.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGKP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(508..1797)
                     /locus_tag="ACGI8A_001816"
     CDS             complement(508..1797)
                     /locus_tag="ACGI8A_001816"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFH0179814.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1877..2581
                     /locus_tag="ACGI8A_001817"
     CDS             1877..2581
                     /locus_tag="ACGI8A_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFH0179815.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2673..3719
                     /gene="selD"
                     /locus_tag="ACGI8A_001818"
     CDS             2673..3719
                     /gene="selD"
                     /locus_tag="ACGI8A_001818"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2721..2723,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFH0179816.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3770..5149
                     /gene="selA"
                     /locus_tag="ACGI8A_001819"
     CDS             3770..5149
                     /gene="selA"
                     /locus_tag="ACGI8A_001819"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFH0179817.1"
                     /translation="MLPSVDEVLSDNRIVEIINEYPRSLVLESVREVIDLKRQFILRL
                     KEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLGRSLLSEDIKDE
                     LWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNAAAVLLVLSTMA
                     KGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYKEAITEDTNVLM
                     KVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKYGLSYEPTVLDS
                     IRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDKLTICALEATLR
                     MYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINIEDGFSQVGGGS
                     MPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDVRTIFDDEFDVI
                     VEELRKAFN"
     gene            5263..7146
                     /gene="selB"
                     /locus_tag="ACGI8A_001820"
     CDS             5263..7146
                     /gene="selB"
                     /locus_tag="ACGI8A_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFH0179818.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7251..9989
                     /locus_tag="ACGI8A_001821"
     CDS             7251..9989
                     /locus_tag="ACGI8A_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HFH0179819.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEHELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10108..11052)
                     /gene="manA"
                     /locus_tag="ACGI8A_001822"
     CDS             complement(10108..11052)
                     /gene="manA"
                     /locus_tag="ACGI8A_001822"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFH0179820.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11225..12586
                     /locus_tag="ACGI8A_001823"
     CDS             11225..12586
                     /locus_tag="ACGI8A_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0179821.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12674..14563
                     /locus_tag="ACGI8A_001824"
     CDS             12674..14563
                     /locus_tag="ACGI8A_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0179822.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14578..15033
                     /locus_tag="ACGI8A_001825"
     CDS             14578..15033
                     /locus_tag="ACGI8A_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0179823.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            15092..15409
                     /locus_tag="ACGI8A_001826"
     CDS             15092..15409
                     /locus_tag="ACGI8A_001826"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:NF014368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFH0179824.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15434..16696
                     /locus_tag="ACGI8A_001827"
     CDS             15434..16696
                     /locus_tag="ACGI8A_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFH0179825.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16801..17865
                     /locus_tag="ACGI8A_001828"
     CDS             16801..17865
                     /locus_tag="ACGI8A_001828"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFH0179826.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17919..19097
                     /locus_tag="ACGI8A_001829"
     CDS             17919..19097
                     /locus_tag="ACGI8A_001829"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFH0179827.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(19288..20346)
                     /locus_tag="ACGI8A_001830"
     CDS             complement(19288..20346)
                     /locus_tag="ACGI8A_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFH0179828.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            20413..20784
                     /locus_tag="ACGI8A_001831"
     CDS             20413..20784
                     /locus_tag="ACGI8A_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFH0179829.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            20821..21495
                     /locus_tag="ACGI8A_001832"
     CDS             20821..21495
                     /locus_tag="ACGI8A_001832"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFH0179830.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            21744..22583
                     /locus_tag="ACGI8A_001833"
     CDS             21744..22583
                     /locus_tag="ACGI8A_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HFH0179831.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            22827..22923
                     /locus_tag="ACGI8A_001834"
     tRNA            22827..22923
                     /locus_tag="ACGI8A_001834"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22861..22863,aa:Sec,seq:tca)
     gene            23073..23780
                     /locus_tag="ACGI8A_001835"
     CDS             23073..23780
                     /locus_tag="ACGI8A_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFH0179832.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(23922..24758)
                     /locus_tag="ACGI8A_001836"
     CDS             complement(23922..24758)
                     /locus_tag="ACGI8A_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0179833.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
BASE COUNT         9853 a         2645 c         4217 g         8079 t
ORIGIN      
        1 ataataatga aaatcaatat caaataaatg ggaggaaatc taatgaactt attagtgatt
       61 ggtggtcatg aaagaatgga aaaagattat atggttatgg ccaagaaaaa aggttataag
      121 acaaaggtat atactaccat gtcatctaaa ttaaataatt ctataggaaa accagatgca
      181 gttgtcatac ttacatcaac tgtttcacat aagatgtcta gaacggttga gtctcaagct
      241 aaaaaacaag atatattaat cgtaaggcat aaaaatagta gtaaggtagc atttaatgaa
      301 tgtctagata tggttgatga atgtctggga aattgttcaa aatgtaagct taaagacaaa
      361 caaaaacaaa aaaatatata ggtataaaaa atacataaac ataaaattaa aagtactgag
      421 ataatagtta taattattat ctcagtactt ttagatttat aaggataagt atagtataaa
      481 ttttttatta taatttccaa ctacttgtta ctttttcata ttttttttac ttctagcttt
      541 ttttcttttt ttcttcttta tatttgaagc tattataaat aaaactaaaa gcactagaat
      601 acctgcaata atcttaacaa aagtcattaa aacattattt tcagtcaaaa atggaagact
      661 gctatcaaga ttattttttg caattaagtc tatactgttt tctagtttac cattattata
      721 tacatttaaa gtaccaacct tagctccttt ttttataggc aaatcaattt tatttaactc
      781 tgttttggta tcataattgc cttgagattc atctttttct aagaatatcc tataactatt
      841 ttttggttca taaatcaatt catgttgttt tgtaaacaat acttttttag tatctgtgta
      901 tttttcttta tcaacaatag tagcacactt aaagttatca aatccataat caatcagaat
      961 acgtgagtct aaatataaat cagcattagt tgaattaaat acagcaacga taactcttct
     1021 gccatctttt acagcactcg ataaaagaca tttaccagca gcatcagtat atccagtttt
     1081 tataccatca actatatcat attttatatt tataggttga cctttgtatg taatcttatc
     1141 atgtgatgtc aaaaacttat tagtgtttac aaagtatctc tcataaggat atgcttttgt
     1201 agcttcaaat ttaagtgact tagtttttac aatttgtcta aaagtatcat tattcatagc
     1261 ttctctagct atgagagcca tatcatgagc tgttgtgtag tgatttgggt ctggtaatcc
     1321 atgaggatta ttaaaatgag tattagtagc tccaattttt tttgcctcag agttcatgag
     1381 gtcaataaat ttttgtacat tgccaccacc aacatatcta gctaatacaa aagcagcatc
     1441 attagcagaa tgaaccaaaa gagcatctag taactgcttt acagtaaaag attccccatt
     1501 ttttagatac atactagagc catctatttg aggtaaatct ttaatttcta ttacttgatt
     1561 taaatcttta acctccttta gaactaaaca agctgtccat actttagttg ttgaagctgg
     1621 ataaagctta gaatttccat ccttattata tagtattttg ccagactcat agtccattaa
     1681 aactgcatgt ttagcaacta tagctggttc attatctgca aaagagatat tagagcttcc
     1741 taaaaatgaa gtaaatatca ataaacagat taataaaagt gataaatttc ttttcattgt
     1801 gagcctccaa attttaaatt catcttttag tatagcatat atttgtcaat aattaaaaga
     1861 aaggagtgat taatatatga aaaaaaaggt aaaatttatt tcgttttttg ttttatttat
     1921 tttaataata tcatcaataa tcgtttataa atcgcttatt ttcaaagatg atatttatgt
     1981 tgtaagtaaa aaaaatttac ctaatgaaaa aacacatgtt gtagagaatg aaattaaagg
     2041 tgaagataat aatcacagta aagtaactaa agacatagat aaaaattttc aaaataataa
     2101 aataactata tacataagtg gagcagtaaa tagacctgga attgtaacta tagagtcaga
     2161 caagagactt tatgatgctg tagaattact aggaggaacg acaaaagaag cagatttaaa
     2221 tggagtcaat ctatcagtaa gattagaaga tgaacaacat tacataatac ctaaaatagg
     2281 tgaagcaaca tctgttacaa gtaatgatga ttccaataaa ctaaatcaga agaatgagtc
     2341 aagaggtgag cctaaaaata aatccaataa aaatatatct aacgaatcaa aagtaaatat
     2401 aaatgtagcg acgatagaag agttggattc tctacctggg gttggagaag ctacagccaa
     2461 taaaatttta caacacagag aagaaaatgg acagtttagt tcaattgagg aaataaaaaa
     2521 tgtaaatggt attggagata aaaagtatga aaatataaaa gatttaattt gtgtagacta
     2581 aaatataata caaaacttac atatagatat gttattataa atatgtatgt atatgtgtgt
     2641 acatgtggaa atttagtaaa ggaggcactt ttatgaaaaa agaacaaaaa aaacttacag
     2701 agatgacaac agcaggtggt tgagccgcta aaatagggcc agaggttctg gcatcagttt
     2761 tatctcaatt gcctaaaaat gataatatag aaaatttatt agtaggacta gatacagcag
     2821 atgatgctgc agtttataaa ttaaatgatg atatggcact tatccaaact ttagacttct
     2881 ttacaccaat ggtagatgac ccatacgtat ttggtcaaat tgctgcatca aattcactat
     2941 cagatgtata tgctatggga ggcaaacctt tggttgctat gaatattgtt tgttttccat
     3001 catgccatga tatggatgta cttgcagaaa tattaaaagg tgggtttgat aaagtgaaag
     3061 aaagtggagc acttttggtt ggagggcata ctgtagatga caaagagcct aaatatggtc
     3121 tttcagtttc tgggattgta agtccaaata aagttttatc aaatgctact gcaaaacctg
     3181 gtgataagtt aattataaca aaaccaattg gagtgggtgt attaaataca gccatgaaag
     3241 aaggtatggt agaacaacat atagcagata aagtaataga aataatgata catttaaata
     3301 aatatgcagc aatgagtttt gagaaatttg atgtaaattc ggttacagat ataactggtt
     3361 ttggtctact tggtcatact ttagaaatgg ccaaagcttc tgaagtaagt attgaaattg
     3421 aatcaaaaca tgtaccaatt attgaaggtg caatagaaat ggcacaaatg ggaattatac
     3481 ctgctggtat gtataaaaat atgcattatg tatctaaaga tgtagaagtt gtaggaaata
     3541 tagaagttgc agttcaagat attttatacg acccacaaac atcaggaggg ttgttaattt
     3601 cagtaaaaga agaacttgca gaggagcttg taaaagatat gaaattaaat ggagcaatag
     3661 aagctaaaat tataggttct gtagttccaa aaggggaaaa atatattaca gttttataaa
     3721 atattataga gaaagagtga ttgttttgag caaaagagaa ttgtttgcaa tgttgccttc
     3781 agttgatgaa gtattaagtg ataatagaat tgtagaaata ataaatgaat atcctagaag
     3841 tttagtttta gaatctgtga gagaagtaat agatttaaaa agacaattta tattaagatt
     3901 aaaagaagat gcatcaaact ctgtaacaat agagtttggt gaaataatag aatctgcaat
     3961 agagagagtt aagttaaatt actcattatc tttaaaaaaa gttataaatg caactggtac
     4021 agttattcat acaaatttag gaaggtcttt acttagtgaa gatataaaag atgaattatg
     4081 gtgtgctgct tcaaggtatt caaacttaga atacgatttg gataatggag aaagaggttc
     4141 aagatactct catttaacta gtacaataaa aagacttact ggagcagaag atgtactagt
     4201 tgtaaataat aatgcagcgg cagtacttct tgtacttagc acaatggcta aaggaggaga
     4261 agcaattgta tctcgtggag aattagttga agtaggtggt tcatttagga ttccaagtat
     4321 aatggctctt agtggtgcag aattagttga agttggttca actaataaaa ctcatttgaa
     4381 agattacaag gaggccatta cagaagatac aaatgtattg atgaaggttc atacaagtaa
     4441 ttatagaata atgggattca cagagagtgt ttctatagaa gaattggtta atttaggtaa
     4501 aaaatataaa ctacctgtaa tagaagactt aggaagtgga gtttttatag atttatcaaa
     4561 atatggttta tcttatgaac caactgtatt ggattctatt cgacaaggtg cagatgtagt
     4621 aacttttagt ggagataaga tgcttggagg tccgcaagca ggtattatag ttgggaaaaa
     4681 agagtatata gaaaaaatga agaaaaatca acttactaga gcacttagag ttgataagct
     4741 tacaatatgt gctcttgaag caacattgag aatgtattta gatgagacaa aagctataga
     4801 aaatatacct actttaaaaa tgttgacata taaaattgaa gaattagaag taaaagcaaa
     4861 taaactattt gaaaaaataa ctgctttaaa cttaaatgca aacataaata tagaagatgg
     4921 attttcacag gtgggaggag ggtctatgcc tcttgaaact ataagtacaa aggtaatatc
     4981 aataactcct gaacatatga atgtttcatc tttagaaaaa aaactaagac ttagtgaagc
     5041 acatataata gccagagtgt atgataataa atatgtacta gatgtaagaa ctatatttga
     5101 tgatgaattt gatgtaatag ttgaggaatt gagaaaagca tttaattaaa taaatttgag
     5161 ttaataaata ttaaatttag aagtataagt atgaagtttt gattaaaaaa tagctacaca
     5221 cacttgtgac tttagtcata gcaggtttag agggggcaaa aaatgaaaaa tgtagtaatt
     5281 ggaacagcag gtcatattga ccacggaaaa actactctta taaaagcatt gactggaaga
     5341 gagactgata cgttagatga agaaaaaaag agaggtattt caataaactt aggatttact
     5401 tattttgatt taccaaacaa tagaagagct ggaattgttg atgtcccagg acacgaaaaa
     5461 tttataaaaa acatgttagc tggagcatca ggaattgata tagttttact tgttgtagca
     5521 gcagatgaag gggttatgcc acagacagtt gagcatttag atattctaag ctttttaaat
     5581 atcaaaaatg gaatagtggt tctaactaag tctgatactg tagatgatga atttagagag
     5641 ctagtaaaag aagatatttg tgaaaaaata aaaggaactt ttcttgaaga tgctgaaata
     5701 attgaagttg attctatatc taaaagaggt atagacactc ttattgaaaa aatagataaa
     5761 atgagtgatg atatagaaga taaaaatgaa aactctccag caagacttaa tatagacaga
     5821 gtattttcaa taaaagggtt tggtactgta ataacaggca ctttaataga aggaaagata
     5881 agtatagaag atgatttggt tatatatcca caggtattaa aaacaaaaat aagaagtata
     5941 caagttcatg gagaaaataa agatacagct tatgcaggtc aaagaacagc aatcaatata
     6001 tcaaatgtaa aagtggatga tataaaaaga ggagatgttt tagctgcacc aaactcattg
     6061 gaggaatcaa tgatgttaga tgtaaaatta tcattggtaa atcatttaga taaaggatta
     6121 aagcactggg atagattaag attgtatcat ggaactagag agatactttg tagagcagtt
     6181 ccactagata aagaattaat tgaaagtgga gaaagtggct atgttcagtt gagacttgaa
     6241 gaaagcatag tatctaagaa aggtgatacg tttgtagtga gacgttattc tccaatggaa
     6301 actataggtg gaggtgtaat aattgaccca tctcctaaaa aacataagaa atttgatgaa
     6361 aaagttatag aagcattaaa aataaaagaa aaaggcgaac taaaagatat aatagaagag
     6421 tacttaaaaa ggaatttaaa aaattatcca aatataaaag aaataatgag ctatagtggt
     6481 gctcatgaag aagatgtaaa aagagcgctt gaaacactta tatcagaaga taaagtattt
     6541 attataggaa atatgtatat gcatataaat caatacaata aattaaaaga aaatacaata
     6601 aagcttttaa gtgagtacca taagaagtat aggcttagaa aagggatact aaaagaagaa
     6661 gtaaggtcta agatagagag taattttaag actagggaga tggatatttt attagaaaaa
     6721 ttgtctacag aaaatgctat aaaaatagaa aataatatag tatctctatt agattttgag
     6781 gttattttaa atgataagca aaaagaaata gcaaaaaaaa tagaaaaaag attaaaatca
     6841 tgtggagttt cttctatact gacaatagat gaagtttctg aaggaaatca taactatgct
     6901 gaagtattgg agtcaatgat aggtaataaa gtagagaaat tagatgattt gtatataatg
     6961 gacaaagata tttatgaaaa tgcaaagaac attttgataa attatataaa agaaaataaa
     7021 gaaataacat taggagaata cagagatttg attgattcta gcagaaagaa ttgcatgatt
     7081 atattggaaa acttcgatag aaacaaaata actaaaagag tagaaaacaa aagaattttg
     7141 ttttagcaga aaatgtctgt ttttgcttta aggtataaag aaggaattat aatgtcgagt
     7201 atcgtataat taattagtat aaaatattaa ttaagaggaa gtgtaaagta ttgaagaata
     7261 taatacctga tgtaaaacca gatataaaag aaagagaaga catgagcctt ttatttataa
     7321 taatgggctc agtactttta ttatttatta taaaagatat agagaaatat gaaactttat
     7381 tacatgccat atatacgata tttttagtag ctacaggagt aattatattt aatactacaa
     7441 aatttaaagt agattcattt tctacatttt taggattaat ttttatagct actggagtat
     7501 tggagtgtat atatttattt aattccttgg ggattaaaac taagtctata atggagatta
     7561 acatcacaat atcagcaata acagatttat ttcctatact gggtgtatac ttaagtttta
     7621 aatttgttaa ggataacaaa caaatttatt caagtgtaat tttatttata gcaactgcta
     7681 cattaacaat aacatcttta ttcataatat caggaatttg tgactatctg ggaaatggta
     7741 aatcacaata tgctttagga gttataatat ctatattcat aataattatt tctatcatta
     7801 gtggaattga attaaattca agttcaaata aaggtaaatg ggaatatgat gaaaaaaaga
     7861 tttttaacag aataatagta ataataatct tatcaagagt tccaaatcta ttgcatatag
     7921 ttattgataa tagaaatgta gagaggatat tatcacaaat aataataaat gtagccttat
     7981 attatttata taattacata gtatctaaga atattaagaa aacagcactg gaattgcatg
     8041 atacgaatga agaattaaca aaaaaaacgg aaagtttaaa agaaaagaat aaaaaattaa
     8101 tctatgagac agaaaagata gaggaactta agggaatact tatgaaaaga gaatctaggc
     8161 tacaatctac attagatgta gcagtaaata gcatagtagt atttagcaaa gatggagaaa
     8221 ttacatatgc aaataagagc tttagaaata cttttggaga ctataaagaa caagataaat
     8281 tgactgataa aataaaaaac ttcaatgatt tgatagaaag cattcattat gtgtttgttc
     8341 atgagaagaa tgcagagaaa cttattagta caagcgataa taaagtttat caagctatat
     8401 ttgcaccttt aattattgct tcacaaacag aaggagtatt gtgtgtacta gaagataaga
     8461 ctaagaaaaa ggagtacgaa cgcaaactta ttgaagcaaa taagagaagt aaagattttt
     8521 tagaaagtat tggagatggc atagtagtgc ttgaaggtaa taacaaaata tatgttaata
     8581 atgcatgtag ggaaattttt aaaaataatt tagagagtat tgatttttca ttagtatgta
     8641 aaagtgaaga aagtatagaa aaaagatatg taatagatgg tgaagtaaag tatgtagaga
     8701 tgagtttttc tcagtatact aatgctggta ctaataaaac tattattgta atacgtgata
     8761 caacctctcg aaaaatatct caaataaaac ttgaggaaag tcaatcatca tattcaagat
     8821 ttatagatat attacctgat ggcatatgct tattaaaaga agatttaagt ataaactatg
     8881 caaataagtc gtttttagat atgctagctt ttacaaacat agatgatata aaagattcta
     8941 atattaaact attaatgaat gcgaatactg aagaaaaaat gaaatttacg gataatatgg
     9001 ctaaagtttt aaatgaaaat agaagtatac ttttactgga acatgaattg ataaatagtt
     9061 atgatgatat agtagaagta gaattaagtg cattaccttt tgctatatac aatactagat
     9121 acataatgct tattataaaa gatttagtac ataagaaata ttccgagcaa gcagaaaaag
     9181 aactgcttga aagatttaaa acagataaaa tcaaaacgga gttttttgca aatatgtctc
     9241 atgaactaaa aactccacta aatgttatat ctagttcaaa tcaactggta gattctttct
     9301 atagaaatga aaagataaaa gattacaata acaatattaa atctcatgtg gatttagtta
     9361 gacaaagttc ttataggctt caaagattaa taaataacat tatagattta acaaagatgg
     9421 agtctgggtt ctatacacta aaacttgcga aatacaatat tgtatctgta atagaagact
     9481 tatttatgaa tatagaagag tatgcactta gaaaagacat aaaaattctt tttgatacag
     9541 accttgaaga aattaatgtt tatatagaca aggttgaaat tgagagaatt atgttgaatc
     9601 tattgtcaaa ctgtattaag tttactgata atggaggctg gatatatgta agtattcatt
     9661 ataaaataga taaagtaatc ataagtgtaa aagacactgg agttggaata ccacaagata
     9721 aattagagtt gatttttgaa gaattttcac aagttgataa gactttatcc agaaatactg
     9781 aaggaagtgg aatagggctt gctatagtta agaatctagt aagtcttcat ggtggagata
     9841 tagaagtagt aagtgaagtg aataaaggca cagagttctt aatttcactt ccaatgaagg
     9901 gatttagtag tgaacattac acagaagata agagaatata taatattcaa gaaaaaataa
     9961 aaatagaatt ttctgatata tattattaaa aatgattatg agcaaaaata aaataaaaat
    10021 actatttgtt taaatattta agttgtaaga aataggctat cataaggtaa attattatga
    10081 tagcctattt ttagctaggt tttatatcta tatgtgagaa catataatct ctaaattacc
    10141 ttcaaaacta aaatctccaa agtcgtttgg cagtataaaa tgcataccct tttttaaatt
    10201 atattctaca gaattgtgta taagttttcc atttccatcc aacacactgt ataaagaaaa
    10261 aggtgtattg tgtgtaaaat tacattttcc aaaaacatct aatttataaa cactaaaaaa
    10321 ttcgtttgac acaaatgttg tgcatttaaa gtctgatgtt gatacgattt tatagtcagt
    10381 atcgaaattt atatgaggaa cattcgtaac atctattgat ttttgaacat gaagttcacg
    10441 cttattacct gaattgtcag ttctatcata atcatagact ctataagtag tatctgagtt
    10501 ttgttgagtt tctaaaatca atgttccttt gcatatagca tgtatagtgc cacttggaac
    10561 atagaaaaag tctccctttt ttatattaac ctttctaagt agtttgtccc attcattatt
    10621 atttaccata tttacaaatt ctttatgaga ttttgcatta tgacctatta taatttctgc
    10681 atcatcagaa caatctatta cataccaaca ctctgtctta cctaactctc cattttcatt
    10741 ttttttagca tattcatcat ttggatgaac ttgaactgat aaattatcat ttgcatccaa
    10801 tatttttgta agtaaaggaa acttatcacc aggtgtgttt ccaaataatt ctctattttt
    10861 attccacaat tcagatagtt ttttaccttt atattttcca ttttctatta agcaatctcc
    10921 atttttatga gagcttatag cccaacattc accagttgtt ggcgaatcta tttcataatt
    10981 aaatttatct ttaagtgcag taccacccca tattctatcc ataaaaatag gttttaaaaa
    11041 taatggttcc ataatcctgt tcctcctatg atattaaatt tatcctacaa ataattatat
    11101 ataaatagat tataaaatca atagtaaaaa agttttgtcc cttgaaatga cataaagtat
    11161 aaggagttat taaataagat aaactatgat atataatata aagttctaaa ttggagtgat
    11221 aattatggag ttgtttaagg cagaaagcaa aaaatataaa aaagaatttt taaaattatt
    11281 ggagcaatgg gttagtatac cttcatttta tgatagaaaa actgtatcta aagacatgcc
    11341 ttttggaaaa ggagtatatg atgcattaaa ctggtttgaa aatctaggta gagaaaacaa
    11401 tttcaaagta aaaaatatag ataatcatgc tgttcaaatt gaatatggaa atggtaaaga
    11461 gtatgtagat atatttggac attgtgatgt tgtaaatcca ggtgaaggat gggatagtga
    11521 accatttaaa ctaaatatta taggtgataa attagttgct agaggtgtat ctgataataa
    11581 agggcctatg atagtgaatt ttttagcact aaaaatgata aaggatttag atattaattt
    11641 aaaaagaaag gttagattaa tagctggggg aaatgaagag agtggattta aatgtataaa
    11701 gcattattat agcaaagagc catatggtgt atgtggattt actccagatg ctaaatttcc
    11761 tgtattaaat ggagaaaaag gtggagcaat aataaagtta atttcaaata tagacgataa
    11821 atctttatat ataagtggtg gaatagagtt taatacaata ccagataaag tttatataaa
    11881 gaatgtagaa aaattgggta aagataatat atgttttgat attaacaata tatcgataaa
    11941 ttatgataat ggcaattata ttgtgtatgg aaaaggtggt cattcatcta aacccgaaaa
    12001 atcaataaat cctatattag ctacaattaa acttttgtca gaaaatattg atgaaaaatg
    12061 gacaaaagat ttatataagt tgataaatca agataatata aatggaaact tatttggttt
    12121 aaatattgag gggaaatgtg gaattttatc aatggtgcca acaataataa atatagtaga
    12181 tggaaaatta gaagtagttt taagcgttag atatccagaa atattaacca ttgaagatat
    12241 aataaaaaaa tttaatttgt atatggagca aaataatatt aataaatttg agcttattgg
    12301 ggaaaattta aagcaagcaa attatataga tagaaattca aaacttgtaa gaagtttgca
    12361 tgatatatat ataaagtatt ctggagattt aaaaaatgat gttagagtaa ctagtgcagg
    12421 aagttatgct tctgaaatga ataattcagt aatatttggt tgtgaatttc ctgatggtag
    12481 ctttggcaat gtccatagtg cgaatgaatt tgctagctta gatagattta taactgcaat
    12541 aggtatatat gcagaggcaa taattacatt atgtaataaa atataaatac aaaatgtccc
    12601 ttatatagac atattatata tacaattaaa attttacttg atatataatt gaaatcaaag
    12661 agaaggtgat acaatgataa ctaagaaaca tgcaataata gtaaagagtt taagcaataa
    12721 agatggatat atgacatcaa acgaattagc tatcaaattg gatgtgtcta ctaaaactat
    12781 taaaagatat atagcagatt taaatagtgt tttaagcaaa tatgatttgg taatagcttc
    12841 ttctagaggt ataggataca agttaagcgg ttctaaaaac aatatagcca gagctgttaa
    12901 agaagcaaat aaatatattg atggtttttt agatgactct gaagagtcaa gaatgtcaaa
    12961 tataatatgt atgcttatta atagaaatta tatgagtata gaagcaatgg cagaagaatt
    13021 aaatctaagt atagccgcta taaataaatt gtctagtaag cttaaaagaa aacttgaaaa
    13081 gtatgactta gttataaaat ctaaacctta ctatggaagc tatatacatg gtgaagaaat
    13141 taatataagg caattaatta ctgactatgc aataaaatta gatgaaaaaa gtaaagtgaa
    13201 ggtgttttta gatgacatta gtgaaaacga tatacactgt atagaaagta ttttagaaaa
    13261 gcacttaaga gaaaaagata caataatttc agataaagat tttaatcttt tgctttcaaa
    13321 aataatcgtt tctgtattta ggagtaagag aggtcatagt aacaatataa acttaatgga
    13381 tacaagttat agatttcata actactactt tatagaaaat ctaatgaaag aaatatctga
    13441 taaaattgga tttaaactta ttgaagatga agtaatttat atttcaaatt attctggtgt
    13501 aatagcctat aaaggtacac aaggaagaaa aaacacaagt gaaattgagg aacgaataag
    13561 tagtgttata agtagtgcac ttcaagatat attccttatt tcaggtagtg attatacaaa
    13621 agatgatgag tttatggttg caatctctga ccatataaag agatttttaa atagagctag
    13681 agctaatgta aaatcaaata atcctttact tcatcaaata aaagagaagt ttcctatagc
    13741 ttttaattta gccgtgtttt tatcaaataa actagaaact gagtttaatt taaaacttga
    13801 tgaagatgag cttggatata tagctatcca ctttgctgct tcaaatgaaa gaatgaaaaa
    13861 aaatactagt aagaaaattt gtatagtttg tcattatggg ataggaacag gacaacttat
    13921 ttctgaaaag ctaaaacaga atataagtga tttaagtgtt gtaggtgttt atccagttag
    13981 atatctcgat atggctataa gtcaagatgt tgatttgata gtatctaccg ttgaattaaa
    14041 aggatatgaa aaacctgtct tatatataga aaatatattt gatgatagct taattgaaaa
    14101 cgtaaacaaa gccttttatg aaaaggaaga aagaagaaag ataataagca acatgtttga
    14161 tgaaaaagct tttttcagta taaaggctaa tactaaagaa gaggtcataa tttcactttc
    14221 aaataaacta aaagaaagag attttataga ggaaagttct ataaaaagta taatagatag
    14281 agaaaatatt tcatcaactg aaataggaaa tctagtagca ataccccaca caatagtgaa
    14341 gggagataaa aaatctataa taggagttgg aatacttgaa aatccaataa tttgggataa
    14401 gcaagaggtt cagctagtat ttatggtatt ttttaatact aaagaaaaac ataatttctc
    14461 gatatttaaa tacctttata attttataaa agatgaaggc ggagtaagag gaattattaa
    14521 aatatgtgac tttaataaat taatggcact gataggtaat taaggaggaa attaataatg
    14581 ggaacaaaca tatttaacaa agaatatgta tttttaaatg ttgatgctaa gtcaaaggtt
    14641 gaagttttaa aatttatatc aaaaaaagct aaagacttaa acttggcaga agatgagagc
    14701 ttagtatatg aaggtttaat ggctagagaa gaacaattta ctacaaattt aggagaatct
    14761 atagcaatac ctcacactaa aaatgatgca attgaaaatc cagcagtagt agtattgaag
    14821 tttaatgaag atgttgtttg gaatgaaggt gaggataagg ttaaattagc aataagctta
    14881 cttatgccag gaaaaagtaa agagaatatt catcttaaat tattatcatc tctttcaaga
    14941 aaattaataa ataaagaatt taaagattct cttttaaaaa gtgataatgt tgaagaaata
    15001 tctaacttaa taaatgaagc tttaggttta taattaaaca ctaaggttat aaaataaaaa
    15061 aataattaac taaaaatagg aggaatttga aatgaataaa aaattagtag cattatgtgc
    15121 ttgtccaatg ggactagctc acactttcat ggcagcagaa gcaatagagc aagcagcaaa
    15181 ggcattaggt tatgaagcaa aagttgaaac tcaaggtgct gatggtgtac aaaatgagtt
    15241 aacaagagat gatatattag gagcaactat gataatacat gcagtagcaa taacaccaga
    15301 aggtatggaa agatttgatg gttgtgaagt ttatgaagtt gaattacaag aagctataaa
    15361 aaatgcagaa ggtgttataa aagaaataga agaagattta ggaatataat aaatttattg
    15421 tggaggaaat agaatggcaa ttaagaaaaa agttataaac agttcaggtg cttctacaac
    15481 aggaggaaat aatgtaggaa aatctactcc atctagtcca actcaatcaa agggaaatgg
    15541 aaaatggaaa gaaataagta aacatataat gacaggtata tcatatatga taccagtttt
    15601 agtaatgggt ggacttatag gggccttatc tcaattaata ccatatgcta tattaggact
    15661 tgacccatct gtaggtatag ttgatgctat gaactcagga gaatttacag ggttcaagct
    15721 tagtctttta aatatagcac aattaatgtc aaatttcgga tttactttgt ttggatttgc
    15781 gataccactg tttgcagcat tttgtgcaaa ctctataggt ggaaaaacag ctttaattgc
    15841 aggatttata ggtggatata tagctaataa acctgtaggt gtaccacaat tcgttgatgg
    15901 acaatggact gaagttgttc cagttgcatc aggattttta ggtgcaatac taatagcatt
    15961 tataatagga tattttgtaa aatggttaaa taaatctata aaagtttctc ataactggtt
    16021 agcttttaaa acaacatttt taataccatt aatagcgtca ttagcttgta tggtattgat
    16081 gatatttata ataactccat ttggtggatt aattaatgaa agtatgaaaa acttcttaac
    16141 agcagcagga gcagctggag aatatgtata tgcaacagct ttggcagcag ctacagcatt
    16201 cgacttagga ggaccaataa ataaagcagc aggattcgtt gcacttggtt taactacaga
    16261 gaatgtactt ccaataacag caagaacaat agctatagtt gttcctccaa ttggattagg
    16321 gttaacaaca ttattagata aaagattagt aggaagaaga gtatatgata gacaattcta
    16381 tcaagcagga aaaacttcta tattcttatc atttatgggt atatctgaag gagcaatacc
    16441 atttgcactt gagagaccag gttttgttat acctttaaac atagttggaa gtgtaatagg
    16501 agctattaca ggtataattc taggagcaat tcaatggttc ccagagtcag ctatatgggc
    16561 atggcctcta gtagataact tatttggata tataataggt atagcagttg gtgctatatt
    16621 catagcagta ggaaatatat tctatagaaa taaattaata aaagatggta aacttgttgt
    16681 agattatatc gactagtatt taatttttac tatctataaa taactttggc tggtaatatg
    16741 gttttaatca atattaccag ccttacctat atgattaatt aaaggcggtg gagataagat
    16801 atggataaga tagaaaaagt aagagagtat tttagagaat atgatataga tggatttctt
    16861 ataaatagtc ctacaaacaa gttttatgtt ggaaatttat ttagctcttc aggttatgtt
    16921 tttataacga aagaatcaca atatataata gtagatttta gatattttga agaaattaaa
    16981 agaaaatcta gtttgtttaa tgtagtatta atggacaaaa ctagaactca ttttgatata
    17041 attaatgata tttgtagaga gcagaatata aaggaaattg gatttgaggg aaatgaagta
    17101 tcttttgatt tatatagaag tatgagtaat aagttaagtg caaccttaaa atcagttgat
    17161 ttatctacat taagggaaac aaaaaatgaa gacgaaataa aatatataaa aaaagcttgt
    17221 gaaatagttg atgctacatt ttatcatata gtagacttta taaaggttgg tatgactgaa
    17281 aagcaagttg aaaatgaaat tgttagaatt ataaaagatt taggtggtca aaaagaatct
    17341 ttcgatacaa tagttgcatc tgggcttaga ggagcattac ctcatggaaa agcaagtgaa
    17401 aaagttatag agtatggaga ttttgttacc tttgactttg gtgctaagta taataattat
    17461 tgttcagata ttaccagaac catttgtatg ggtactataa ataaagagtt agaagaaata
    17521 tataatatag taagaaaagc taatgaagag tgtataaggg ttttaagacc gggaatgact
    17581 actggagaga tagataaagt tgcaagagac ataattggtt catatggata tgctggtaat
    17641 tttggtcata atctgggtca tggtgtagga attatggttc atgaatatcc tgcattagct
    17701 cctgaatcaa atgaagtatt aaaagaagga atgattgtaa ctatagagcc tggtatatat
    17761 gttccaagtc ttggaggcgt aagaatagaa gatgatgtac ttataacaca agatggatgt
    17821 atgagattga ctacatctac taaggattta atagttgtaa gctaaaaata aattagataa
    17881 atattttata acaataagaa aagaagaggt aaatttaaat gtataatttt gatgataaac
    17941 cagatagagt aagtgaaaag tgtagaaaat gggatttaaa tattataaga gataaatttg
    18001 gagatattag agaagatttt ataccaatgt ggattgctga tatggatttt aaaataccaa
    18061 cagaaataga aaacaaattt attgaagctg taagaagagg tgtatttgga tatacatact
    18121 gttatgatga attttatgat tcagttattg gttggcaaaa aaatatgcat gaggtaaatg
    18181 tagaaaaaga atggattaca ttaacttatg gaactgtatc aacacttcat tatgtagtac
    18241 aagctttttg taagaaaggt gatagtataa tattaaatac accagtttat gacccctttg
    18301 aatcatcagc aaaaaaacaa ggtgtaaatg taatttgtaa tacattagat gttgtaaatg
    18361 atagatatta tataaatttt gataaattag aagttcaaat taaagaaaat aagcctaaat
    18421 taatgatgtt ttgtacacca cataatccat ctggaaggat atggacaatt gaagaaatga
    18481 caagggtagc aactatatgt aaagaaaata atgttatttt ggttgcagat gaagtacatg
    18541 cagagcatat tcattatggt acatttaatt caatacttaa gattggaaaa gagcttttag
    18601 aaaatgttat tctattgaca tcaccaaata aaggatttaa tcttggtgga ttaaagactt
    18661 cttattcaat agttataaat aaggatataa gggacaaatt tagaaataaa ttaaagcaaa
    18721 attctataac ttctccaaat gtgtttggaa taataggatt gataaccgct tataatgaat
    18781 gtcatgagtg gttaagtggt gtaaatgaat atataaaatc aaattatgaa cttttagaaa
    18841 cttgggtaaa taaatataat aagataaaat tgatgaaaat ggaatcatct tacttagctt
    18901 ggatggatat aagtggatta ggtattagtg caagtgagtt tacagataaa ttggctatag
    18961 atacaggagt attattagaa gatgggagtc attttgtaaa agatggtgag aagtatgtta
    19021 gaataaattt aggaactcaa aaggaaaatg taattgaagc tttaaataga atggatttat
    19081 ttttaaagtc tttataaaaa tttggttgaa ttagtatttt atataaattt aaactattaa
    19141 tatggtatat acataagtag aatttgtttt agattactta agtatatacc atattttata
    19201 taaaaattaa atttgcatta gtcatgtgat aactatttaa attcatatgt attttgtatg
    19261 tataatagat ataattatta tttaaactta ttgtttattt aatttttcaa tagaaaaaga
    19321 tttatctgta agtttaaaat tatcttttat acccttagtt aagtatactt tcttatcctt
    19381 agttatgaat atagcatcta cattatcaat tttattaatt aaattcatac ctttttcaag
    19441 accaagacca aatgtagaag tggataaggc atcacaatta atagatttat cagagattat
    19501 tgtaacacta cttaaattgt tttcaaaagg ataccctgta aatggattta acatatgatg
    19561 atatattttt ccgttttttt cgataaatct ttcataaatt ccagaagtaa caacagactt
    19621 atttgtagtt tctatgtttc ctatggattc accattttta ctagttgggt cttgaatacc
    19681 tattttaaaa ggtttatcat ttttaccatt ttctaaagta aatatatttc ctcctaagtt
    19741 tactaaacct ttttttacgt cctctgattt taaataggct acaattttat cagcagcata
    19801 tcctttagct atggcaccta aatcaatttt catattttct ttggttaatt ttatagattt
    19861 atttttttca tccaatatga tgttcttata atcaataaga ggcagaagtt tttgtatctc
    19921 agaatcactt ggaactttag ctttatctgt tccaatagcc cataaatttg ataatggacc
    19981 aattgttatg tcaaaatatc catctgataa tttggaatat tgtatagctt cttttacgac
    20041 gaaaaatgta tcatcagaaa ctctgacaaa ttttttacca gcattatcat ttattttaga
    20101 aacatcactt cctggtatat gagtgctcat tttgttgtca atatatctta aaattgaatc
    20161 acattcattt aagattttat cacttttgga ttcttttaca ttaaatacag taacttcgtt
    20221 tactgtacca aggtagtagc tagttttact atatttagta ttctggcttg aactcctaaa
    20281 aaatgaaaat aaaattaatg ctaaaatcaa tatcacaaaa ataaatgtga tttttttctt
    20341 attcatggta tattcctcct atgaaattgt gttattattt tattataaca taatttcata
    20401 ggaggacaaa aaatgaagaa aaaagacatt cttcttattt taggcatttt ggttgttatt
    20461 acagcatgtt atgggataat aaatgttata aattctaaaa atgctggaaa tattgagatt
    20521 tatgtagata ataaattgta taaaactgtt tcaataaatg ccaaagaaga atttaaaata
    20581 gagaatagag gggggtataa catagtaaag attcatgata aaggggttga gatagtagat
    20641 gcttcatgtc ctgataaggt ttgcgtacat actggattta taaacaagcc aagtcaaagc
    20701 atagtttgta tacctaatag agtgagcata aaaataaata caaatgaaaa gaatgataat
    20761 caagaagata taatttctaa ataaaataat aaaaataatt gaaaggaatt ggtgataatt
    20821 atggttaact taggaaacga ttgggatgaa ttattgaaag aagaatttga aaaagattac
    20881 tacttaaact taagaaaatt tcttatagaa gaatataaaa cacgccaaat tttcccaaat
    20941 atgcacaata tatatgaagc tttaaaacac acttcataca aagacacaaa agtcttaata
    21001 ttaggacaag acccttatca tggagataat caagctcatg gtttagcttt ttcagttcaa
    21061 ccacaagtga aaactccacc atctttatta aatatgtata aagaattaaa agatgattta
    21121 ggttgtttta tacctaataa tggatattta atgccttggg cagaccaagg agtgttactt
    21181 ttaaatactg ctcttactgt tagagcgcat gaagctaatt ctcataagaa caaaggatgg
    21241 gaaattttca ctgatagagt aatatctatt ttaagtgagc gtgaagaccc agttattttt
    21301 gtactatggg gaagtaatgc acgtaaaaaa gtagagttga ttgatacaag taaacattat
    21361 attttggaag cacctcatcc aagcccattg tcagcaagta aaggcttttt tggatgtaaa
    21421 cacttctcta agataaatga aattttgaaa aagctaggaa aagaacctat taattggcag
    21481 atagaaaata tataaaagta aaaggtaaca aaatgataac aaaacaacaa taaacaatga
    21541 cattgttgta actaatagaa atatgcttaa atagttgaaa atactatgta tgcatatttc
    21601 tatttttttg tcaagttgac aactcttgaa aaaatagtta caatttagtt acacaaaaaa
    21661 cacaaataaa aaaagaaact tacaaagcag aattttatga agcaaaatat atagtgtaaa
    21721 aaatcttagg gaggaaattt tatatgaatt ttaaaaaatt gagtaatgct aaagtctcgg
    21781 taatggcagt tgttttatca gttggaatat tatcgggata ctctgcgctt aataaacaag
    21841 taacattagt agtaagggga gaagaaagaa ctatatcaac atttagttct aatgtaggag
    21901 accttttaga atctcaaaac attagctatg atgcaaatga tatagtaact ttagatgaaa
    21961 atacaaagtt atctaatgga gataaaatag aagtaattga tgtaaaggaa aaaacagttg
    22021 tagaaactaa agacattcca tttgaagtta cagttgttga agatggctct ttattaaagg
    22081 gtgattctaa ggttaaagaa gaaggaaagt ctggtaaaag tgaattaata tataaagaaa
    22141 cttatcacaa tggaaagaaa gtagaaaagg aatttgtgaa aaaggttgtt aaagctgagc
    22201 cagtaaataa ggttgtaagc aaagggacaa aggtagaaat gcaagtagcc tcatcaaggg
    22261 gagaaagttc aagaagaatg gcttcatcat catctagtag cagtagtagt aacaatggta
    22321 aaaatatgag agtggtagca actgcatata ctggtcacag tataactgca acaggaacaa
    22381 aacctaagtg gggaacaata gctgtagacc ctaaggttat accatatgga actaaagttt
    22441 atatacctca atttgacatg gtgtttactg cagaagactg tggtggagca ataaaaggaa
    22501 ataaaattga tatctacatg aatgattcaa aaacagttta taactgggga agaaaaacta
    22561 tagatatacg tatattaaaa tagttaaata atttatattt ttactggaaa ttatattttg
    22621 attatataga gaagaaactc tttatagatt ctaaaaatat aatttccagt tttttatttt
    22681 ggatatgtaa taaaacaggt aaattttaac catgtataga taaaagctat ttaaattata
    22741 atttactatc atataaatct ataaaaattg ttgcaaagag tcaaaatata tgatattata
    22801 gatttcgttg agaaggattt cattatggga gtggatgggt gccggtgtgc cctctagtct
    22861 tcaaaactag tatgaggggt taagagcctc ttaggtgggt tcgattccca cgcattcccg
    22921 ccaatatgta aaaggtatct tttaattaaa agataccttt ttaaatttgt tttaagataa
    22981 tttagtttta atgatatata atattttgta ttatataatg taaattttat aatactatta
    23041 ttgttcagat aaaagtctag gagagtgaaa agatggaaaa taattttaca acgagaacta
    23101 gttttctagt tggagatgat gggatagaaa aacttaataa ttcaaatata atagtatttg
    23161 gagttggagg agttggtagt tttactgttg aagctttagc aagagctgga gtaggcaata
    23221 ttactattgt agattttgat gatgtagata taaccaatat aaatagacaa ataccagccc
    23281 tacattcaac tgtaggaaga tacaaagttg atgtcatgga agaaagaata ttagatataa
    23341 atcctaatat caatataaaa aaaataagaa gtctttataa taaagatact agtgacgaaa
    23401 tactaactga aagatatgat tatgttgtgg atgcgataga catggtaagt tccaaaatac
    23461 atctaattga aacttgtgaa aaaaaaggat tgaaaataat aagttctatg ggtatgggaa
    23521 ataaattaga cccaactaaa atagtcgtta cagatataca caaaacaagt acttgtcctc
    23581 tagctaaagt tatgaggaaa gaacttaggg atagaggtat caaaaagtta aaagtagtat
    23641 attctactga acaaccaata gaacttaaga agaaagtaat gaatggtaga aaagtaacac
    23701 ctggtagtgt atcatttgtc ccatctgtag gaggtttaat aatcgcatct gtaatagtaa
    23761 atgaactatt aggacaataa gttttaaata ttttaattta taaattttat atacaaattt
    23821 gaaggctaga aggatgaata taaagcaatt ttatattcat acttttagcc tagttatttt
    23881 atattacctt attttatgta gttgtagctt tattttttag attatgtatc tatttcataa
    23941 ttatattttg taaatataat ttctccatca ctataaaaaa caatatctcc tattctgtct
    24001 gttctataaa ttgatacatg gttttgtttt agattattaa taacctccct atgtggatga
    24061 ccatattgat ttttatatcc acatgatatt gcagctatat ctggactaat tttttttata
    24121 aattctaaag atgaagaaga attacttcca tgatgaccta tttttataaa attaatatta
    24181 tttaatttaa aagaatgtaa aatttctttt tcattttctt cttcagcatc acccataaat
    24241 aaaaaagaat tatcattaaa agttaatttg aaaactatag aatttaaatt agattcttct
    24301 tgtatatatg atggacttaa tacatatata tttatattgt tatctatgtt taaaacatca
    24361 tttttataga gatgctgaat agataagttt ttatccgtac aagaatttat tagattttga
    24421 taagcctcac tatcagttga ttgttcaggc atatatatgg agtttacatt aaattttttt
    24481 ataatattat caagacttcc tatgtggtct gaatctggat gagttgctat tataatatca
    24541 atagttttta ttcttttttg tctaagataa ctaataatta tattttcgct atcttcatca
    24601 cctccatcaa ttaatatatt cttatttgtg ggtgtttgaa ctaaaatact atcaccttgt
    24661 ccaacatcaa tcatatgtat agatagtaga gattttttgt cacagccgat taaaagtgat
    24721 attataataa gtattgataa aaaaatttta tttctcaata aaaaatcact ccattcatta
    24781 catgtttata taaa
//
LOCUS       DAYATN010000048        23461 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.048, whole genome shotgun sequence.
ACCESSION   DAYATN010000048 DAYATN010000000
VERSION     DAYATN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23461
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.048"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..893)
                     /locus_tag="ACGI8A_001837"
     CDS             complement(<1..893)
                     /locus_tag="ACGI8A_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFH0179834.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKS"
     gene            complement(933..1280)
                     /locus_tag="ACGI8A_001838"
     CDS             complement(933..1280)
                     /locus_tag="ACGI8A_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179835.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1498..2454)
                     /locus_tag="ACGI8A_001839"
     CDS             complement(1498..2454)
                     /locus_tag="ACGI8A_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFH0179836.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2899..3267
                     /locus_tag="ACGI8A_001840"
     CDS             2899..3267
                     /locus_tag="ACGI8A_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HFH0179837.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            3692..3937
                     /locus_tag="ACGI8A_001841"
     CDS             3692..3937
                     /locus_tag="ACGI8A_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179838.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            4036..4299
                     /locus_tag="ACGI8A_001842"
     CDS             4036..4299
                     /locus_tag="ACGI8A_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HFH0179839.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(4584..5531)
                     /locus_tag="ACGI8A_001843"
     CDS             complement(4584..5531)
                     /locus_tag="ACGI8A_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0179840.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLANTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5534..7165)
                     /locus_tag="ACGI8A_001844"
     CDS             complement(5534..7165)
                     /locus_tag="ACGI8A_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0179841.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            7295..8212
                     /locus_tag="ACGI8A_001845"
     CDS             7295..8212
                     /locus_tag="ACGI8A_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0179842.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            complement(8269..8850)
                     /locus_tag="ACGI8A_001846"
     CDS             complement(8269..8850)
                     /locus_tag="ACGI8A_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFH0179843.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(8890..9456)
                     /locus_tag="ACGI8A_001847"
     CDS             complement(8890..9456)
                     /locus_tag="ACGI8A_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFH0179844.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQINNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            complement(9912..10772)
                     /locus_tag="ACGI8A_001848"
     CDS             complement(9912..10772)
                     /locus_tag="ACGI8A_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0179845.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            10921..11811
                     /locus_tag="ACGI8A_001849"
     CDS             10921..11811
                     /locus_tag="ACGI8A_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0179846.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            12029..13783
                     /locus_tag="ACGI8A_001850"
     CDS             12029..13783
                     /locus_tag="ACGI8A_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HFH0179847.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLIMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            14167..15153
                     /locus_tag="ACGI8A_001851"
     CDS             14167..15153
                     /locus_tag="ACGI8A_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0179848.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            15489..16970
                     /locus_tag="ACGI8A_001852"
     CDS             15489..16970
                     /locus_tag="ACGI8A_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFH0179849.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGAIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            16996..17967
                     /locus_tag="ACGI8A_001853"
     CDS             16996..17967
                     /locus_tag="ACGI8A_001853"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HFH0179850.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(18223..18825)
                     /locus_tag="ACGI8A_001854"
     CDS             complement(18223..18825)
                     /locus_tag="ACGI8A_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0179851.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPTLWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            19106..20317
                     /locus_tag="ACGI8A_001855"
     CDS             19106..20317
                     /locus_tag="ACGI8A_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFH0179852.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            20385..20582
                     /locus_tag="ACGI8A_001856"
     CDS             20385..20582
                     /locus_tag="ACGI8A_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HFH0179853.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILSLYIKQLGVTDVSLI
                     QQYSGIVFGCTFVVAAFFSPI"
     gene            20616..21587
                     /locus_tag="ACGI8A_001857"
     CDS             20616..21587
                     /locus_tag="ACGI8A_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0179854.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            21774..22151
                     /locus_tag="ACGI8A_001858"
     CDS             21774..22151
                     /locus_tag="ACGI8A_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0179855.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            22982..23341
                     /locus_tag="ACGI8A_001859"
     CDS             22982..23341
                     /locus_tag="ACGI8A_001859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179856.1"
                     /translation="MLLVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKAS
                     FKNFKISKKEIYICIPMIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNIS
                     IFVLIHIILKIKIRKIR"
BASE COUNT         8987 a         2836 c         3398 g         8240 t
ORIGIN      
        1 aaactctttt tatctcctaa ttttaagata aaatcaggat attcttcaaa ttcattgagt
       61 tttattaatt tgaaagaaga agatagattt ttaaagttaa taattttaga gttattcact
      121 aatacacttc tttttttaaa ataaatttct tttttactta tatttagtat agttaatatt
      181 tgtttaagat atattattat atcttctgga gttctatcaa atttgagttc aaaattttca
      241 tcttcaaagt agggaatata atataaaggt gttttgagag caggtaattt attatataat
      301 tcttcaagaa tatcgatatt tttaggggag ttaaagaaaa agcatttaca tacattcatt
      361 aaatccacag catctgaaag tgagttatgt ttaatatctc cttctacaga aaaaattttt
      421 ttcatattca gtaaagatat ttgcttagat aagattctat tattgtatgt aatatgttga
      481 gatataaaag gttgaatgtc aatcatcata ttaaataact ctaagcattt acctgtatat
      541 ttatatactc taaatgaagt tagaaggcca gttatatcaa aatcacccca attatatatt
      601 gtaacatcat caaatttaca aaaccattta taaaaatcat ccataacata taaaagatta
      661 ggagcattat tgatatctag ttggttcaat ttggttaaat ttttacattt atttgaaagt
      721 attttattgt attttggctt aactaaagag taataattat ctattgtatt aaattcgtta
      781 tcaactaaaa ctccaccaat agaaattaat tccgcaccat tatattcttt tggtttattt
      841 ttattatcat ctagtatatt aaattcaaag tctataaata acttgtattt cataaatcta
      901 aagcacatcc tcaggtaaat tatttattga gcctaagcat cataagaatc aatagatata
      961 atgactctaa acatcaaagg ttcattacaa cttttattta tttcataatt taattcaaat
     1021 aaatctttta agatataatt tataagtatt ctttcccaac ccatttttaa attaacttta
     1081 gagtctatta atatattttc tatcttttta aatatttcta tatttgctgg tattatactg
     1141 agctcaatat tacttttaaa cgggttagac tgtaaatctg ttataagtat attttttaat
     1201 tcctctgtaa gttcagaaat taaactatca cctatgtatt tttttatgat ttcctctcta
     1261 atttctttta aatttagcaa tattagtacc tgctacttac atagtagctt ttcctttcct
     1321 tttaatacta ttattcattt ttgaatctta tttagatata ttttaagatt aaaagtcttt
     1381 agctatataa ttttttaccc atttttaact tatattaaca tattacaggc ttatatctcc
     1441 accctgttta cattgaggat ggagtatatt gcctattttg ataaaaatga taggttgcta
     1501 tttgcctatt cctttttctg ataatttttc taacatatct ttggtcattt tatctaaatc
     1561 ataactaaac ttaaagttcc attcttttac tgcactacta ctgtctagag agttaggcca
     1621 agaatcagct atactttgtc ttacagggtc tacatcatat tctatcacaa aatcaggaat
     1681 atattttttt atagaatcag ctatttcctc aggagaaaag ctcatagcag taatattaaa
     1741 agcatttcta tggatgagtt tatcagctgg agcttcgatt aaatccacta ttgattgaag
     1801 tgcatcaggc atatacatca tatccatttt tgtaccttct gctatgtaag atttatatct
     1861 cttatttttt aatgcctcat aatatatatc aactgcataa tctgtagttc ctccaccagg
     1921 aggcgtaaca taagaaataa gacctggaaa tctaacacct ctagtgtcta ctccaaattt
     1981 tgagtgataa taatcacata gtaattctcc agaaacttta gtcacaccat acatagtatt
     2041 aggtctttgt aatgtatctt gaggagtcat attttttgga gaattttcac caaaagcagc
     2101 aattgaacta ggtgtaaaaa atttacaatc caattctttg gctatttcaa gaccatttat
     2161 taatccattc atatttaagt tccaagcttc tagaggttta ctttcagcaa ctgcagaaag
     2221 taaagatgct agatgaatta tagtatctac atcatatttt tttgcaattt caaaaaaacg
     2281 attcttatct gtaacatcta atatctcaaa tataccagat tcacatactg gattaccttc
     2341 ttttactctc cttgaactag cgattacatt ttgttctcca tactcatttc taagctttat
     2401 tgttaattct gaacctattt gtcctaatgc acctgttata agtatttttt tcaattttac
     2461 aacctcctaa gggtttaatt tatgtagtac tacttatttt tattttagca aatcttttgt
     2521 ttaaatacaa agaattaaag ataataatat acgacttaat tgaatgaagt gcagtaagtg
     2581 cctaaaaatt taaagctaat atataatggt atattgtata acaataaaag agtagattaa
     2641 attattatat atagattgtg tgactaagtc acagaaaata gagttaattt aatatataat
     2701 aaaagcatat caagtaaagc tttaaattaa agtaacttta taaataaaaa gtaatataaa
     2761 ttgataaaat tataaattta tttatagtga atatgggttg gattttatag atacaaaaat
     2821 agtatatatg atatgtagac cattagcagg tttaaagtag caaaacatat aaaataaaat
     2881 ttgaaaggaa gtataaaaat gacaagacca tcaatatatc atagccaagg atacacttgt
     2941 gcagaggcat taataaagtc ttataatgag gaacataaca cagatatacc aatctcaata
     3001 ggaagtggta tgggggttgg aatgaatgtt ggaagtgttt gtggagctgt aaatgctgct
     3061 gttgttataa tagggtattt aaatgggcga aatagtaatt tagatgaaaa tatagcaagg
     3121 aagtattcaa gagagctatt gaatagagta agaaataaat acagctcaga aatatgtgta
     3181 tctcttaaaa aaaataaagt aacttgtgaa gaaataataa atttcacata tgatgctcta
     3241 aatgatatgc taaaaaatca aaaataattc taagaaacaa gtattttaca ttcgatagaa
     3301 tacttgtttt tttatgatat ttttaataag tattagttta ttataatttt taaatcaatt
     3361 ttattatatt ttaattgaaa caatctactt tgaaaattac taactgacaa aagaatatga
     3421 ttatagtata gaaaaaatgt aaattattta atattaagtg agagtatatg tttacctata
     3481 taaaatcgta taaattaatg gctaaattta taaaatataa agaaatatat aggaaaacaa
     3541 aagaattaat tagtataata taaagtttaa aagttaaaat ttatataatt tatttataaa
     3601 gtaaagaaaa aatagaaatt tgtaataaaa taaaaagttt atgacataat atggtataat
     3661 tagtaaaaat atagagattg gaggaattgc tttgtttata gatgaagaat tagaaggtta
     3721 tatattaaca tgtaaaatat ctgaagactt taaaaatata cctgaatata gtgatgaaga
     3781 gttttatgtt acagtctata aagatgaaag ttctgactct gggtactatg ctttattaga
     3841 aaataaagaa gaaagagttg tatgggatgg agaagttgtt gccaataata tttttaataa
     3901 cctttggatt gtagtaaata aggttaaaac tggataatag tctaattata tagtatttgt
     3961 aatattttaa atgaattttt acatatttga gatgagtaaa taacaaagat tattcgatac
     4021 tggaggagta attcaatgga atatagttat agtaagatga acttaaaaaa aggggatatt
     4081 gtagaggtaa atttagagaa acaagccaat gtaatattgt tagaccatat aaattatgtg
     4141 aaatttaaaa atcaaagaaa ttatgattac tatggtggat ttgcaaagaa aaatccatgt
     4201 agaatgagag ttcctaatac tggaacatgg tatctagtag taaatcaaga tggaaatagt
     4261 ggaatagtaa acttttcaat caatacaatt caaaattaat tttagaatat tggatattct
     4321 taatttttgt acattttata tagttaaaat aaaattaata agcaattatt ataaaataat
     4381 gtgggtgcct caaaatgaat tttataattc atgagacact cttttttata tgaactcaaa
     4441 tatactttat catttcaaca atgaaaaaga ggcgtatctg tgttttgaga tagcctcatt
     4501 tatcatatat catatataga attttaatta ttcttaacta aaattttttc cataggttat
     4561 ctgtacaatt aaaatattta ttgttagcat acagctccac ctgtaaggtg taaaagagcc
     4621 tctagtatct cttcttttct ttccaaaact tcatctgata aaagcatttc tataaattta
     4681 tcagcaccga ttctatcgat agttattcca aaacgttcac ctgtgttacc ctgttctcta
     4741 aataaaagaa ttgctttttc aattaaagtc ataagttcct cttttgaaaa taatctatca
     4801 atctgtgttc caggacgaac tgattttccc catttccctc ctatatagac tttataacca
     4861 gattcttttt cttcaatact atcaaaatta caactttcta tacacttacc acagttatta
     4921 catagattac tatctatttc aagtttacct ttatctgtta atttagctgc tttaacagga
     4981 cagacctcta caacagcaca ttttttacaa ccaacacaaa gctctgaatc atagtcaggt
     5041 acacgttgac caacaattcc aagatcgttt aggtcaggtt taatacagtt attagggcat
     5101 ccaccaacac caattttgaa cttatgaggt agttttacat cataccaatt tttgtaaaat
     5161 tcttcatgta tttcagcagc aagtgcctgt gtattagcta gaccatgaac acaaacagta
     5221 cctttacaag ccacaacagg acgaaccctt gatccagttc cacctgttat tagattttcg
     5281 ctagcaataa attcacttag agcttcaata tcttcaaatt ttacaccagg taattctact
     5341 gttagtctag tagtaaatgc taactgaccg tttccgaatt tttcagcagc ttctgcaatt
     5401 ttttttgctt gtgatgcatt tattacacca tttacagtta ttatacgtgc tgcaaaatgt
     5461 tctccatccc tgcttggtag aaaacctcta ccttttactt cctttttttg ttctgctgta
     5521 acattactca tatttactcc tccattacct taagatttgg ataaaatagt attccacctt
     5581 caagaacttt cgtatttgta tagccaaagc gtcttaagcg attttgcgcc atatagctat
     5641 ttttaccttt agcacatata agaagaattt tttcatcctt agcaagacct tcaatttctc
     5701 cattaatttc accaacagga atataacgta aactaggaat agaaggagct tttgaaacat
     5761 caagtttagt ccaactgtca aaatcctcaa tttcgtcaag taaaacacta tcaagttctc
     5821 cattaagttt attttgtaaa acatttactg ccactacaaa agggtgaata gctgttgaaa
     5881 aaggtggtgc atatgcaagg tccatacttt ctaatgaata aaggtcagcg cctaaagtta
     5941 ttgcagtagc agcgatatct actattttat caaccgctcc aggtcccata acttgaacac
     6001 ctaataattt tctagtattt ttttcagcaa ccattcttat aataaaatta gatgcacctg
     6061 gataataatg agctttatca tcagtagcta tggttacaga gcaaacatta taaccttctt
     6121 tttcagctat ttctttactt agacctgttt ttcctgcatt gagctcaggt aattttacta
     6181 ctgttgttcc aagaacacca ttgtaagttt tagattttcc agatatattt tgtgcgcaaa
     6241 ttctaccctc atgatttgca gatgacccca taggtgacca tgtaggttta tttgttaaag
     6301 aattttttac aaaagcacag tctccaacta catatatatc tttgtcattt gtaagcatat
     6361 attcatctgc aataattgta ttattagggg caaattctaa acctgtgtct tttagaaaac
     6421 ctgtgttagg acggattcca actgacataa ccactaaatc tgttttaata gctctatttt
     6481 tagtacgaag tttttcaact ttattttcac cttcaatacc tacaacctgg tcacctgtaa
     6541 aaatcattat gcctttatca gccatatgat tttcaacgta ttcagcaaaa tcagtatcaa
     6601 atccaggcat aacatgttca gccatatcaa caacacttac acgtataccc attgcagaaa
     6661 gattttcagc aatttcaaga ccaatgaaac ctccaccaat aacaacagct ctcttaatac
     6721 cgttttcaat agaatctctt agttttatag catcatttgg agtacgcata aagaatactc
     6781 caggtaaatc tattccaggt attggtggtt ttattgggtc tgcccctgta gcaattacaa
     6841 gtttgtcata gcttagattt tctttttctc ctgttgaaag cgttttcaaa gttacttgtt
     6901 ttgcatttct atcaatagat gtaacttcaa ctccacaacg aacttctgct cctgttagtt
     6961 tagaaaaact ttcaggtgta tttacgatta aagatgattt atcttcaatt atcttaccaa
     7021 cgtaatatgg aagaccacaa cctgcataag aaatatcatc acctttattt agtattgtaa
     7081 cagagcaatt atcacccatt tcacgtttta tttttgcagc agtttttgtt ccagcagcaa
     7141 ctccacctat aattaatact ttcacaatat atcacactcc aatctacttt ataactctat
     7201 aataaaatta tacttcttga caatatattt gtaaaatact aaaatattaa taaaatcata
     7261 agcatttact aatgtttgct aaggttgtga taatatgaca attcgtagtc ttgaaatctt
     7321 tgttaaggtt gctgaatgtg gtaaaatgag tgaagtagca agaaatatgt atattaccca
     7381 gtcatcagtt agccaagcta tttcagaaat agaaaaagag tatggtgtta agctatttga
     7441 tagaatttca aaaaaattat atttaactga ggcagggaaa aaacttcttg gatatgggag
     7501 gcatctttta gctgtaaatg aagaaatgaa tgattgtatg aagcattgtg caaaaaatat
     7561 ccgtataaga gttggggcaa cagtaactgt tggtacatgt gtaataagtc caataatgtt
     7621 agaattatac aaggtaaatc ctcttataga acctgaagta tttgtagaag atacaagact
     7681 gattgcaaaa aaattactta atagtgaatt agatattgcg attgtagaag gaaaaattaa
     7741 gcacccagat attgttacaa aaagcattat taatgataat cttgtactta tatgttcaca
     7801 caagcatgag ttttataaaa gagactctat aaaagtttca gaactttcaa atcaaccatt
     7861 aattatgcgt gaattgggaa gtggtacaag agcacaatta gaaaagcaat taaaagaatt
     7921 aaaaatccca atgaatatta gatggtcatg ttataactct gaagctatat taagagcagt
     7981 tgtagataat tttggcattg cagttatatc cgaattactt attgaggatt atttaaaaaa
     8041 gcatcttttg tgggcttgtg atattgaagg tattaattta cataggacat ttgatattgc
     8101 atatcataga agcaagtttt ttacagaaaa tatttcagca ttttttgata tttcagtaga
     8161 atatgggaaa aaacaagcga gaaagaagag tagaagtgta aacagtttat aatatatcta
     8221 aataaaaata agagtatatc aatatactct tatttttatt tagatatatt agcctatatt
     8281 aacgccaaaa tttttacaca atcctgcaag tccatcctca aatccagaac ctaaagcatt
     8341 aaatttccac tctccattat gtctatatag ttctgccaca actattgctg tttctatgct
     8401 aaaatcttca cccaattcat atttaataag ttcctcatta gtttcttcat tatatattct
     8461 tatatatgaa ttttcaactt gtccgaaatt ttgtcttctt gtaattgctt catttatagt
     8521 aacactaaat gatattttag caatattttg aggtatttta gataagtcta catttatttg
     8581 ttcatcatca ccatcaccaa cacctgttct attatcaccc atatattcaa cagctccaga
     8641 agcgtgtttt aaattattat agaatataaa gtctccatca ttcgttactt ttccatcagt
     8701 tccagttaaa aatgcagagg catctaaatc gaaatcaaag ccaccatcat atttattaat
     8761 atcccatcct agtcctacta ttactttttt tagaccaggg ttactttttg ttaaacttac
     8821 tttttgtcct tttgctaaag ttatacccat ttaaaccaac tcctcattat aaatttttta
     8881 ttgtaattat tacttatatg tattaattaa ttcacgaagc tcttcatttt gaccaattcc
     8941 taaagcatta aatttccatt cttcatttct acgataaatt tctcctgcca aaactgttac
     9001 tttaccagaa aagtcttctt gaagattata tttaaataat tcttgcttgg aatttgaatc
     9061 aattaatctt ataaaagagt tgtttatttg accaaaatcc tggtctcttt ttttagcttt
     9121 gtatatagaa actccaaaaa ctattttatg tacatattca ggtatgtctt ctaaattaac
     9181 aattatttgt tcatcatcac cttcaccttt tccagttaag ttatctccag tgtgtataat
     9241 agctttagaa gggtgtttaa gatttccaaa ataaaccaag tcacctctaa cattcatttt
     9301 atcttggtta gtcaatgcta ttgcaaatgc atctaggtca taattttttt tacccaattt
     9361 attcatatcc catcctaagc ctactactag attttttaca ggattattat cttttactaa
     9421 gctaactctt tgtccttttt gtaaactaac actcatttta agttctcctt tttatatttt
     9481 tagttttatt gatggtatca agtataagaa ccatattagc tatattatat acgtatttag
     9541 gctaagttgg ctatatttat aaattattta ttatataact acatgaatat tttataattt
     9601 aaacatattg acattatatg ctttaaatta ttttgtagtt ttataatatt taaatttgta
     9661 tttgattgtt ataaagtaaa gtaagtaata aagttagact caataattct aaaatttcta
     9721 agcatactat ctaaatgaga acggatttgg aaatacttgt ataaaacaat aattgataca
     9781 tttaattttt atcttaaaaa caacaattat aatatataaa tagcaagtat aatatataaa
     9841 tagcaagtat aatataacat ataaaaagtt gaatttgtat atgttaatta tacttgctat
     9901 ttatttgtta gttattctat ttttttaaat ccattaccta aagcttcatg tatatctgta
     9961 attataataa aagcattagg gtcgacattt tttactaatt ttttcaattc gacaacttct
    10021 ttcttttcaa ctactactaa aagtacattt ttattttctc cagtatatgc accttctcct
    10081 tttagaatag ttgcacctct atctaaatct tccattattt ttttacttat atcttttgaa
    10141 aggtcagata taatagtgaa tgatttggaa taattaaatc cttcaataag caagtcacta
    10201 acttttacta aaatgtataa agccacagtt gaataaagtg caatttcaac ctgtctattt
    10261 actatacctg ataaaacaac tacaacgcag tctacaatac ccattaaaac aggtatacta
    10321 aatgtaggaa aatatttatt tagtaggagt gccattaaat cagttccacc tgtagagcca
    10381 tttattctaa aaataagtcc ttgaccaact cccattaaaa tagaaccaga tataattgca
    10441 agatacatat catttgttat atctaatgta gccatatttg cagtaagctt aagtgctatg
    10501 gtaagaaaaa ttattcctaa aaccgtttta aaacaatctt tttttgaaag tattttgtaa
    10561 gcaaatatga ataatggaac atttaaaagt aaattgacaa tccaaagttc tacaccgaat
    10621 aatgaattta aaacaactcc aagtcctgtt ataccaccag cagcgattgc atgtggatta
    10681 aaaaataagt taagagaaat ggccattaat atacagccta caattaatat taaaatttct
    10741 gaaaaagtta ttctatcttt tttcaataac atcactcctt atatatagat tataatatat
    10801 attatatagt ttttccaatt tttaataaaa gaaataaaaa tatattataa aaacaatttg
    10861 attggaattt tcagaaaatt aatgctataa ttaaaagtaa atttaaaggg aggttataaa
    10921 atggctaaat taactctaga acatataaaa ggaaatacat attatattcc actccctaca
    10981 atagtaggtg tatacgtaga tggtaaggat gcaattttaa ttgacagtgg aaataataag
    11041 gatacagcta gacaagtttt aagattatta gaagaacata atttaaatcc taaattaata
    11101 ataaacactc attcaaatgc agaccatata ggaggaaatg catatttgaa gaatcaaaca
    11161 aaatgcaaga ttgctacgac taaaattgaa ggatatttta cagaaaatcc aatattagaa
    11221 tcagcatttt tatatggtgg ctatccaagt aaagcactga agaataaatt tttattagca
    11281 aaggaaagtg aagttgatta tattatccct tcaaatggca aaattgtaga tacagaactt
    11341 gaagcgattt ctcttccagg tcattatttt gaaatgattg gtgttagaac tccagataat
    11401 gttttgtttg ttgcagatag tcttacacca gagaatataa ttactaaata tcattttttc
    11461 ttcctactgg atatagattc acagtttaag acactagaaa aattgagaat gttagaggct
    11521 gatttctttg ttccaagtca ctctgtcaaa actacagata taaaaagttt aattaatatt
    11581 aataagaaga agatggaaga aataatagat aatattaaaa aggtttgtta tgagccagtt
    11641 atgattgatg aagttattga aaaaatgtgt gatttgtaca atgtcaagct tgatgctaat
    11701 caatatgtgt tggttggaag tacaatacgt tcttatataa cttatttata tgagaataat
    11761 atggttgaat atatatttga tggtggtaag atgatgataa aagctgtgta aatagtaata
    11821 attttcaaat tatatgatga aattgtttta gtatgactgt tatttgaaaa gtaatataaa
    11881 aaaatttata taaataagct aagatgattg atataaagtt actgataatt ttaaggtaat
    11941 atatacgtta ctataatatt atgttattat ttgtaaataa ggggataatt tataagcaaa
    12001 ttaaagacct taatttacag gaggaatagt gtatattatg aataaaaaat tcaaaaaatt
    12061 cagtaaaaaa ctaagtatat taattttagt tttgattatg tttcttttta ataatttaac
    12121 tgcaaatgca cagattaaag aagattcaat aataggaatt gatagatatg aaacagcagg
    12181 acttatagca gatagacaaa cttatgatac tgtaatatta gttaatggag ataaaaattt
    12241 atctgatggg ctttcatcaa gtggattagc tggagctatt aacgcaccaa tacttttgac
    12301 taaaaaaagt gaaataccaa aagctacttt aaatagacta gataataaga gtttaaatgt
    12361 tgtaaaaaaa gtatatataa ttggtggtta taatactatt gaaaattcag tagaaaaaga
    12421 tataaaaaga aaaggtattc aagtggaaag aataaatggt aacaatagaa ttgaaactag
    12481 ttataatgta gctaaaaaaa taaatgaagt aagtagtgta aaagaggtaa tgcttacaaa
    12541 tggatttgta ggagaggctg atgctatgag tatagcacct gtagttgcaa aaaataaagg
    12601 agctataata cttacagatg ggaaatcagt tccatttaag actgaaagtt taaatgttta
    12661 cgctataggt ggcaaatctg cgataaatga ggatttagta aatgaaacta aagcaactag
    12721 aatcggagga aatgatagat ttgagaccaa taaaaaggta atggaaaagt tttataatgg
    12781 agcgacagat ttttatataa caaatggata tcaattagta gatgcactaa cattatctcc
    12841 tttagccaaa gaaaaaccaa tcgttttagt tgctgatgga agtaataaag gtattttaaa
    12901 aggagctaaa agtataacaa aagttggagg tattgatgcc aatatatata aacagtgttt
    12961 agatatgatt aaatataata atatggatat gactccaaat attttaaaac acttaacttc
    13021 agttgaagga aatgaagttt ctgaaatact tatagaatca gataatttag gtgtggttgt
    13081 tgaaaagacg aataccaata aatttgagtt tgattacgta agtgtaacta atgaaaaaaa
    13141 ttgtactttt tctgtatata aagaaaattc ttctaataat ataaaaaatg gaaagctagt
    13201 tgtaagtgct aagaaaaaaa tagataagaa agatagtgat ttgcaagata tgaatggcga
    13261 caatatgata aatgcaaata aagataggat ggtaaatgtg ataaaaattg gagttcccga
    13321 taaaatgtat tctaatttta aagtagatgt tgaaagtggt tcagtagaac tttataatat
    13381 taaaggagga gctactgttg atgtcaatga cgggattgct aacattgttg ataacaatgt
    13441 aacaaatcct ttcaatataa atactaatag tgggatttca ggtgtaacag ctgaaactat
    13501 atcatctgaa ataaaattta gagcaaacaa tggaattgta aatgtaacag ctacgaatat
    13561 aaatggcaat ataagtttat ttggaagcga taataaaggt acctttgatg gaatatttaa
    13621 attaaatctg aaaaaagaac catcaaattt acacttgaag ttaattggaa atgggataaa
    13681 taaattgcca gaaggttggt ctaaagatta tattttagga aatggacaac caattattga
    13741 agttaaaaat aatggcataa ataatatatc aattggagaa taaaaacata tgaaaataaa
    13801 gtttatatag ttttaaaaat tatagaattt taaaatattt atatatattc tttaaataaa
    13861 aaattatttt ttaagtgata attaagtgtt tatcgataag gctatggttg taaaaatcat
    13921 agccttattt ttatattata aataaattag agtatttatc aattcatagt agataaaatt
    13981 gaaattaaat ataacaacaa aaaatgaaaa ttacaattaa tttaatgtta aagactcaat
    14041 tatttggatg tggaatggat agtacaagta gtatataaaa agtattgact ggacaaataa
    14101 ctatatttta ttttaaatat ataatataat gtaataaaat atatcaaatg tagaaggtga
    14161 taacctatga aaaaaataac aataaatgat attgctaact tggcaggagt atctaaaagt
    14221 actgtatcaa gatatctaaa taataaagat atcagtgatt ctactaaaga gaaaataaag
    14281 acaataatag atgaatatgg atatgagccg aatgcatttg ctcaaagttt gagagctaaa
    14341 aaaacatact ttattggtat tataacaccg tgtttagatt cttttgtaaa atctaaaatt
    14401 atgatggcaa tagatgaaga actcaaagaa ttaaaatata catcactaat tattaatact
    14461 aatagaaaaa taaggtcaga aatagatagt atatcaaaat tagctagtct aaaagttgat
    14521 ggaataatac taattggtac cgaaataact aaagaacata agaatgaaat tgaaaagtta
    14581 gatataccga tagttgtagt tggacaaaaa gttgatggta tcaatagcat agtgaatgat
    14641 gattatggag caggatataa aatgggtcaa tatattgcta ataaaggtta caaaaacatt
    14701 gtatatttag gtgttgatga aagtgatata tcagttggtt taaatagaaa aaatggagta
    14761 cttaatgggt taaaagataa agggtatgat gcaaaagtat tttatactga ctttgaccaa
    14821 gaaacgtcta tacaaagaag tggagaaatg ctagaaagtg aaaatccaga tataataata
    14881 tgtgcaactg ataatatagc aatagcaaca atgaaagaaa tcaataagag aggtaaaaat
    14941 ataccacaag atatatctgt agctggattt gggggatatg atgtattgtc tattataagc
    15001 cctaaactta caacaataaa gtttgaaaat aaaaatgcag gtaaagtagc agcaaatacg
    15061 atagtaaatt taatacaaga aagaaaagaa ccactattaa aggagattaa atttgaatta
    15121 atagaaggtg aaagtacaat aaataaaaat taagatacac attacatata ttagtaatgt
    15181 gtattttttt actaaaaaaa ctataattct aaaaattagg aaaacttttt tgaaagaaat
    15241 attgcatctg aaataaattt atgctaatct ttaattaaac cggtttcgaa accggtttaa
    15301 ttaaaccgat agaatagcaa aatgagtgca aaaataactg attgtaacaa agtatcaaag
    15361 taattattag agtacatagg aataaacaaa atataaaaga tgtagcacat tgtgtaataa
    15421 gactaagaat tgttttagat agcaataata aagcaaatat aaaattataa aagaaaagga
    15481 ataaaaatat gtacaaagag cctaaatata gaaccatatt agaatcgaca aaagaagaat
    15541 taaataaatt aagaaatata tctttaaatg ataaatataa accactattt catatacatc
    15601 cacaacatgg actacttaat gacccaaatg gattagcata ttataatggt aagtatcatg
    15661 tattttatca atggtatcca tatgatgcaa ctcatggaat gaagcattgg gcttatgtaa
    15721 gttctgatga ttttgtaaac tggaatagag aagatgtagc attaatacca atcgaaagtt
    15781 atgaatctca tggagcatat tcaggtaatg cgatagaggt agatggaaaa ttgcatatgt
    15841 attatacagg gaatataaaa tatagtgcag aagatagata tgcatatcaa aacttagcta
    15901 taatgaacaa ggatggtaaa ataactaaat atgaaaataa tccaatagta agtgaaatac
    15961 caaaaggata tacaggacat gtaagagacc caaaagtatt taaaagaaag gataaatact
    16021 ttatgttact tggagcacaa acaagtgata aaaaaggagc tataatagta tatgaatcta
    16081 aaaactctat agattggaat tttaaaggag aattaaatgt aaaaaatata gatgaagatt
    16141 ttggttacat gtgggaatgt ccagattata ttaatataga tgaaaaagat atattaatat
    16201 tttcaccaca aggagtagaa cctaaaggat ttgattatca aaatatatat aatgtagtat
    16261 atgctattgg taatatggat ttagataatt taacatttga aatagataca atgaaagaac
    16321 tggaaaaagg atttgatttc tatgctccac aaacatttat aaaggactca caaataatac
    16381 tatttgcatg ggctggaatg ggagaagttt tataccctac agataaaaac aaatgggcac
    16441 attgtctaac tgttccaaga aaattaaata taaaaaataa taagctattg caaatgccag
    16501 tagatgaatt aattaaatta agatatgatg aaacaagtgg gcaaaatact ataaagaata
    16561 atattaatat tatagaaaat gatgaaaatg tatatgagct taatattaat ataaaaaata
    16621 ttgacagcaa taaatttggg ctggaattat tttcatcaca agatgaaggt gtaaaattag
    16681 aatttaacaa attaggaaac attgtaactt tagacagaag caactttaaa aaagttttcg
    16741 gtgttgaata tggaacaaat agaaaagaat atataaatat tgatgaaaat actaatatca
    16801 aagtactagc ggatagaagt attttagaaa tttttataaa tgaaggagaa gtagtattta
    16861 ctagtagaat atttgctaaa gaaaattcta atcaaataag ggtttatagt gataaaatag
    16921 tagagtatga gtatactaaa tttaaattaa agcaaggtat tgaattataa aaacacatta
    16981 taaggggtag aaaagatgaa aaaagtaatt agcataggag aagctttaat agactttata
    17041 ccgaatcaaa atggtggtaa attaaaaaat gtatcagaat ttagaagagt agcaggagga
    17101 gctccagcaa atgttagtgc tgttgtagca aagcttggag gaaaatcgag ttttatatct
    17161 aagttaggaa aagatgcatt tggtgattat ataatagatg tattaaatga agtaaatgta
    17221 aatactgatt atgtattgag aacaagtaaa gcaaatacag gacttgcttt tgtatcatta
    17281 aaagaagatg gtaatcgtga tttctcattt tatagaaatc caagtgcaga tatgctttta
    17341 gaagctgatg aagttaaaaa agagtggttt aataattgtc atatcctaca tttttgttca
    17401 gtagatttaa tagatagtcc aatgaaatta gcacataaaa aagcaataga atacgcactt
    17461 gaaagtaata gtataataag ctttgaccct aatataagat tacctttatg ggatagtgag
    17521 caatcttgta aaaaggcaat atcagaattt ttaccttttg ctcatattgt taagatatct
    17581 gatgaagaat tagaatttat aacaggagaa aatgatatag aaaaatcatt acataaactc
    17641 tttgtgggga atgttgaact agtattatat acaaaaggaa aagatggagt agatgcatac
    17701 actaaaaaag taaaaggtat gtgtaaaggg gttaaagtaa atgcaataga tacaaccgga
    17761 gcaggagatt cttatatagg ttcattttta tacaccttat tgtataaaca aataacattg
    17821 aatgatataa aagaaatgaa acaagaaacg ttaaatgagt atttagaatt ttctaattat
    17881 tatgcagctc gtagtacaac tattaaaggt gcgataagtt catatgcaac aaaggaagaa
    17941 ataactgaat ttataaaaaa tttatagtag gaatgagtaa taggttttga aggaagtaac
    18001 atgtaaagag tttttaagga tgtttactat gaaaataatt aacatataaa aagcaataaa
    18061 gaggctgtct caaaatagag ataagcctct ttatcattgt tgaaatgata aaaaatattt
    18121 taattattgt tactttttag aagaatatat atcttatttt aaataagcaa attatgtaag
    18181 atttatttat attatcaata ctttacttgg agtattatgt ctttagcagt tatctacttc
    18241 aattgaatta tctaaaatat aataaatttg ttttgcaaat tgacgagaat gttcttcttt
    18301 ttctttattc ataaaattag gtttacctaa atcacgttca acaagcattc ctatatattt
    18361 taactgtgaa tgcttacagt agcgcttcat accattttcc caaatatcag cacccttttc
    18421 aggtctatat ccacatgttg taataagtgc tactggtttt cctgcccata gagtggggcc
    18481 tttctcatta ccatagtatt tattcattcc atacaccaat ctatcaagta gtgctttcat
    18541 tggagaagtg cagtaccaag aataaattgg agtagcaaaa acaattaaat cacacataag
    18601 aattttcttg aaaattttct gaacatcatc tttacaataa catccaaaat caaataagtt
    18661 atcctgacat tttttacaag caatacatgg ctctatttta caatcatata accatattac
    18721 ttcaatattt gaattataat tttctaattc ctcaataaaa ggttttaaaa tagcagcagt
    18781 attacctttt ttcttaggac ttcccataag aatgcatact tccatattga tacctccaca
    18841 gtattttaaa atagttttta gtgcttaata tagttttagg atactatgag aatacgttaa
    18901 caacaataca ctttgtatat ttgtaataaa attgaaacaa attgttttat caataaaagt
    18961 tggaatgtat ctataattaa aaatatttta ggtatgtttt tacatatatg tgtgtagtta
    19021 actatggtaa tttattgtaa aattatctta taggattata attgcttaat ttatgattat
    19081 atttaaaatc ttggaggtaa aaaatatgta ctcaaatgaa gactataaat ttatagaaga
    19141 aaatgcacca tgttatatat atgatgagac taatataatt gaaaaatgta aggaattgaa
    19201 aaataatatt ttaggttttg attttttata ttctataaaa acaaatccat ataacaaaat
    19261 tgtaaaaagc attgcagagc aaggttttgg agcagatgct tcttcaacta atgaagttat
    19321 gatttcatca gaatgtggag tttctaaaga tgatatttat tattcatcac caggcaaaac
    19381 agagattgat atagaaactg ttttagataa atgcataatt attgcagata gtattacaga
    19441 aatagaactt ataaataaga tatctaaaaa gtataatcag aaagttaaaa taggaataag
    19501 aataaatcct gattttacta tggatgatga tagtgctaaa tcaagcaaat ttggtataga
    19561 tatagaagat atgagcagtg tttttaagat gttaagtatg tgtgattatg tatctgtagt
    19621 tgggattcat atacatatta aatctcaggt attggatttt gaaaagttag gacattatta
    19681 tgaaaaatgt ttttttatag ctttaaattt aaataaaagt aagtatatta atattgaatt
    19741 tataaatttt ggtagtggac ttggagtagc ttatgataat cttagagaaa atccacttaa
    19801 tcttaaagag ctttcaaaat taactcaaaa gattgctttt actaattcaa acacactaaa
    19861 atcaagactt cttattgaat caggacgttt catagtttgt aaatcaggta attattatgt
    19921 taaaattgta gatattaaag tatcaagagg gaaaaaatat cttattgtaa aaaatggact
    19981 taatggattt atgagaccta ttatttctaa tttaattgaa aatattactt gtggagtaga
    20041 attagaaggt caagagccat tatatacatc aaatcatgcc ttttctcttt cagtattaaa
    20101 taatttaaaa gaagaagagt atgtaaatgt tgttgggaat ctctgtacat cattagatgt
    20161 aattgcaaat aatgttaaat tgaaaaaagc aaacatagga gatttgattt caattagtaa
    20221 tgctggaagt tactcctatt ctttatcacc gctattattt tcaagttatg aatttcctaa
    20281 acaatattat aaaagaaaag atggatatat tgagtaaaca atagcataaa ttaataaact
    20341 tagtattatt gaaaaaattt aaaatgcagg gggtgtaatt ctatatggaa ttgtggaaaa
    20401 gaaatttatt tgtatgttgg ataggtatgt tttttagttc tatagggatg agccaaatag
    20461 cacctatact ttcattatat attaaacagt tgggggtaac tgatgttagt ttaatacaac
    20521 agtattcagg tatagttttt ggatgtacct ttgtagtagc agcattcttt tcgccaattt
    20581 gagggaaagc tgctgataaa tatggaagaa aatcaatgct tttacgtgca agtattggga
    20641 tgggaatagt tatatttacc atggcgtttg ttcaaaatgt ctatcagtta ttaggattaa
    20701 gaatacttca aggagttttt acgggttatg ctactgcatg tactactctt attgcaacac
    20761 agacagataa aaatcattca ggatgggctt tgggtactct tgcaactgca tcaactactg
    20821 gctcattaat tggtccaaca gtaggaggat atatagaatc aatcttaggt cttaaaagta
    20881 catttattat aacaggagga ttgttgtttg tatcatttat catctctatt ttatttgtag
    20941 tagataattt taaacctaaa gaaataaaag aatcaatttc tatacctaaa gaacaattaa
    21001 atatattgcc aaataaattt ttaattgcat ctatttttgt aaccacattc attactcaac
    21061 tagctttata ctctatagaa ccaattgtaa ccatatatat atcacaactt acaaattttg
    21121 catcaaatgt agctttaata gctggattga cattttctgc atcaggatta gcaaatttat
    21181 tagcagctca aaaacttgga aaaatgtcgg ataaaatagg tcctcaaaaa gtattgttaa
    21241 tttcactttt atgggctggg attatattta taccacaagc ttttgtaaaa actgcatggc
    21301 aactgatgtt gttgagattt ttattagggt tgtcagttgc agggcttaat ccttctgtta
    21361 acagcttgct taaaaaaatt gcaccagaag aatatgtggg aaaaatattt ggatataatg
    21421 catctgccca gtatataggc tgttcatcag gagcattttt agggggacaa atatcagcac
    21481 atttaggtat tagaacagta tttttttcaa caagtttatt gttgtttatg aatgcacttt
    21541 ggatgtataa atttactgga ttgtttatta aagataagac taaataatca tactatttac
    21601 atttctaatt ttatgatgta aaataaatat tttttatgta atttatcata gaattatata
    21661 aaaaataacc ctatggtaaa aattggaaaa tattattatc gacaaaatct ttgacttaaa
    21721 aactatattg cgatagtatt aatttaataa aagtttgaga ggggcgtaag gttatgacaa
    21781 tttcaaaaat accacaagca gaattaaaag ttatgaagtt tatttgggaa aaaaatgata
    21841 cagtaacatc aaaagaaact attgaagcta tggaacgaaa atatggttgg aaacaaacaa
    21901 ctacattgac acttttatca agacttgtta aaaaacattt tttaagtgca gaaaaaatag
    21961 atagatacac tcattataca atagttgttg gacataagga gtatttaagt gttgagacaa
    22021 aagatttttt aagcaatata cacgacaatt ctttacagag tttaatttca gcactacatg
    22081 atgatgaagt tgttgataaa gatatgttgg atttatttga aagtcatttt aaaaatttag
    22141 aagaagagta attatatcag gatagaactc taagaattta attaatctat ggctgaaaac
    22201 cataaatggt attatttaat cagctactca ctattcataa agtaatatta aataggttct
    22261 ctaattagta aaatgcctaa aataattgga atagggagta tcaagttaaa tgataccact
    22321 tatttaaata agaaatgatt tttaaaattt aaaataattt tataacttgt caatattcat
    22381 taatctaaag tattatttct atttaaccat atcaaaatat attttactat ttttaattat
    22441 tacaatataa acctgtcttt tgttattata ggtaaactaa aagtaagaaa tgatttttat
    22501 atttttaaac tttattattg gtaacttatc tgtaaatatt ttaaaatgta ctatagaaag
    22561 ttgttaaaaa tattaaataa ttatattaaa tttaagtgtg ttttttatgc tataattagt
    22621 atgtattttt tatgatatat tttataaaaa atataattta atcatttttg aggatattat
    22681 tatgttagat gcatttataa caattgtttc ttctattata gaaatatggt catgtaaaaa
    22741 agtttttgat tatactagtc agtcaaagac cgacttaaaa aaactaaata tgtattttct
    22801 cttttctgtt tttctcatta tttcttcttt atatataaat ataggagcta atagtagaat
    22861 cttgatatgt atatttgtaa tgttcgtttt ctataagtct aactataagg ttaatatata
    22921 taaatgtctg gtaatctcat ttttttattg gttaatatta ataggtataa atggattaag
    22981 tatgttatta gtagcaacag taagcaattt ggataatatg aatgttttac ttagtggcag
    23041 tttatataga tatgaagcaa tattcttggg aaagataatg ttaggtatat tactttatgt
    23101 ttataaggct tcttttaaaa attttaaaat aagtaaaaaa gagatataca tatgtattcc
    23161 tatgatagca aatttattgt cattttttgt actttatgat tatattatta atcttgtaaa
    23221 aatctctcct gaaagaagaa ctgctttttt agtaatagtt ttactattaa tattatctaa
    23281 catttcaatc tttgtattaa tacatataat acttaagatt aaaattagga aaataagata
    23341 aattttaata cgattcattt aaattaaaag ttaataatta cttttttatg tataatttta
    23401 catgaatttc tataaaagtt attttattaa agatagatat acttatatat acggatataa
    23461 t
//
LOCUS       DAYATN010000049        23315 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.049, whole genome shotgun sequence.
ACCESSION   DAYATN010000049 DAYATN010000000
VERSION     DAYATN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.049"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(122..2194)
                     /gene="ptsG"
                     /locus_tag="ACGI8A_001860"
     CDS             complement(122..2194)
                     /gene="ptsG"
                     /locus_tag="ACGI8A_001860"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HFH0179857.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGELLDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(2497..3339)
                     /gene="licT"
                     /locus_tag="ACGI8A_001861"
     CDS             complement(2497..3339)
                     /gene="licT"
                     /locus_tag="ACGI8A_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFH0179858.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(3600..5642)
                     /locus_tag="ACGI8A_001862"
     CDS             complement(3600..5642)
                     /locus_tag="ACGI8A_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFH0179859.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(5794..6765)
                     /locus_tag="ACGI8A_001863"
     CDS             complement(5794..6765)
                     /locus_tag="ACGI8A_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179860.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(6766..7743)
                     /locus_tag="ACGI8A_001864"
     CDS             complement(6766..7743)
                     /locus_tag="ACGI8A_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179861.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            7962..9521
                     /locus_tag="ACGI8A_001865"
     CDS             7962..9521
                     /locus_tag="ACGI8A_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0179862.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGESKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            9627..10586
                     /locus_tag="ACGI8A_001866"
     CDS             9627..10586
                     /locus_tag="ACGI8A_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0179863.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPAYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLAKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            10589..11401
                     /locus_tag="ACGI8A_001867"
     CDS             10589..11401
                     /locus_tag="ACGI8A_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0179864.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(11617..12519)
                     /locus_tag="ACGI8A_001868"
     CDS             complement(11617..12519)
                     /locus_tag="ACGI8A_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0179865.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            12874..14052
                     /locus_tag="ACGI8A_001869"
     CDS             12874..14052
                     /locus_tag="ACGI8A_001869"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFH0179866.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            14061..14717
                     /locus_tag="ACGI8A_001870"
     CDS             14061..14717
                     /locus_tag="ACGI8A_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HFH0179867.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            14717..15382
                     /locus_tag="ACGI8A_001871"
     CDS             14717..15382
                     /locus_tag="ACGI8A_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HFH0179868.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            15487..16263
                     /locus_tag="ACGI8A_001872"
     CDS             15487..16263
                     /locus_tag="ACGI8A_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HFH0179869.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            16325..17659
                     /locus_tag="ACGI8A_001873"
     CDS             16325..17659
                     /locus_tag="ACGI8A_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFH0179870.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            18155..19087
                     /locus_tag="ACGI8A_001874"
     CDS             18155..19087
                     /locus_tag="ACGI8A_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFH0179871.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPNNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNNNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVAT
                     KKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSE
                     GHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(19479..23018)
                     /gene="nifJ"
                     /locus_tag="ACGI8A_001875"
     CDS             complement(19479..23018)
                     /gene="nifJ"
                     /locus_tag="ACGI8A_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFH0179872.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPVNT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMGICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         8038 a         3729 c         3181 g         8367 t
ORIGIN      
        1 tcaaaatata atgatatata tttctattac ttaaaatata tattaaataa attgcatgtt
       61 aactgaacct atttatatat ttattaaatc tcacataact tttagttaca ctatcatata
      121 gctatcctat tttttcaata tcatctctta atatacttac tctattctcc tcttttgctg
      181 tggttactcc ttttttaatg cttaatttct gattttgttc taaattcgta aatactacag
      241 gacttattgt tgatagacca tttttttcaa tatattctaa gtccatttta actaattttt
      301 ctcccgcttt tacttcatct cctacattca caaatacttc aaatccattt ccttctaagt
      361 ttacagtttc tatccctaaa tgtataagta tttctactcc acttttttgg gattttatta
      421 ttattgcatg cttagttgga aatataactc caatagttcc atcaactggt gaataaatca
      481 atccatcact agatttaatt gcaaatccat cccccatcag tttacttgaa aatacagaat
      541 ctggcacttc ttctatatct aataactctc catttgttaa tgaatcaatc ttttctacta
      601 aagtattttc catatttgca acttccatat tttttttcac tttattttta tcaacatttt
      661 tagtaacagt actttcaacc tttgtttttc cctctattat atcttttata tcagacttta
      721 ttgaatctga ttttggtcca aatatagctt gaacattgtt gccaataacc ataacacctg
      781 ctgctccaag tgatttcaat ttttctttat ctactttatt tatatcattt acaaccaatc
      841 taagtcttgt tatacacgca tctagactct ttaagttgtc tttatttcct aaagcaatca
      901 aaatctctga tgctaattca ttcttagaca tttcaatgac ttcagcgtta gtttctgact
      961 catcttctct tcctggtgtc tttaaattaa attttcttat cataaatcta aatacactat
     1021 agtataatac tgcaaaacca atacctacca agatagccaa gtaccatgga gttctattag
     1081 gaagaacacc aaataataag aaatctatta atcctcctga aaatgtgaat ccaattttaa
     1141 ctcctaataa ttgcataatt gcgtatgata aacctgaaaa taaacaatgt attgcaaata
     1201 atactggtgc aacaaataaa aacgcaaact ctattggctc ggttattcct gttaaaaaag
     1261 aggttaatgc tgctgatgcc attatacctg ctacaaattt tttcttttct ggctttgctt
     1321 catgcaccat agcaagagct gctgctggaa gaccaaacat cataaatgga aatgcacctg
     1381 tcataaaagt acctgctgtg aaatttactc catctttaag ttgagcaaag aatatctttt
     1441 ggtcacccat tacaagctca ccagctttat ttacatattc tccaaattga taccaaaatg
     1501 gattatggaa aacatgatgc aaaccaaatg gtattaatag tctttccaaa gaaccttcca
     1561 taaatgctgc tagtgtttga tttgcttcta taacattttt tgagaaacta agtaatccct
     1621 cttgtattgg aggccaaata aaagacaata taattcctat tgctatagca actaatgaag
     1681 taacgattgg tacaaatctt ttacctgcaa aaaaacctag atactgaggt aattcaattt
     1741 tataaaactt tttataaaga taagcagcca ctatccctat aataatacca ccaaataccc
     1801 ctgtttgaat tgttggtatt ccaacaatat ttgcatacat agaattgtct accatatctt
     1861 ctgtaacacc tgccataaca cccatagatg tattgagaat taaaaaacct actatggctg
     1921 ccaatgctgc aacaccttct ccttcagtta aaccaacagc taccccaact gcaaatataa
     1981 gtggtaagtt attaaatata atttgacctg aactttccat tatacttatt ataagttgaa
     2041 atgtaggatt cgccaaagct ggaaaatgag ccaacaatgc gtctcctgct aaagcattac
     2101 tcacgccaag taatatacct gctgcaggaa gtaatgcaac tggtagcatt agagattttc
     2161 ctactttttg taaaacacca aatacttttt tcatttactt ttctctcctt attattttat
     2221 atttttttaa ataaaaaaga cttaagttta taaaaagtta cattagtata cttaattcta
     2281 aaattaaact tatgttaata taaacaaacc tatattaacc taatttcaga attaaaaata
     2341 aactattata atttttttat aaacataagt cttgcctgca ttatcagtaa caatcttaat
     2401 gttttaaaat tattatttta tattaaattt taaataatat attaaaatta aacataatat
     2461 attaaatcta tacattctgt attaaactac tctttattat aaattcagta cagatataac
     2521 tcttttgata tgtaatgtta aataaagtag ttcatcttga cttacatagt aatcataatt
     2581 cttttctaca tgagttttta ttttatatgc acaatcataa tccttttcat actttacttt
     2641 aattatatct ataatttcat tatttgtatc attgattttt tcttttttta ctaatcgttt
     2701 tgcaaagaat tttaaatgag tcagaagcct atcataattt atatcatctt ctttaaattc
     2761 aactctataa taatatctta taatatttag tatgtctttt acaattttag taattaacag
     2821 tgattccttt gaacttccct tatagtttga atttactata tgcattgcta taaaaccagc
     2881 ttcatctact ggaaatttca catcaaattc tttatttata taatctacag cccatttccc
     2941 aatctcatat tcttttttat gtattctttt tatctcatgt agtaaatcat tctttatagt
     3001 tatgttttca tgatatcttt taattgcaaa tgcaatatgg tcagtaagtg ctacataaat
     3061 atattcataa agtttagtat tcaaattctc ctctgcataa gcaattattt cagtactaac
     3121 ctcaaaaaat ttctcatcaa cctggcttgc tagtttacta attttatcta aaaagctttc
     3181 atcttgtatt acaaaagttc tttcaatttt acttttatct atttcttgac ctttcttttt
     3241 ttgaaaccca attccacatc ctgttaatat taattctttt tttatatttt catctaaaac
     3301 taatacaaca ttattattat aaatttgttg aataatcata atcaatatct ctcccaaaat
     3361 acaatttccc ttattcataa aaaaatacct aaattaagat acaattcacc tgtatacctt
     3421 aacctaggta ttgcctgctt accagtaaca atccagcttg ttaacgctta tatattgtat
     3481 tcaatttatg gttttattct attataaata aatatatatg tcaagtaaat tattgaaaac
     3541 tatttcctat tttttagtat taattttaac atcaaaaatc aaattttatt ccattatatt
     3601 tatgattcta tcatcatcat ttcatcatag agaatatttt ccaataactc tttagccact
     3661 tctcttgata tttgcttagt ttcaactaat tgttgaacac tgtctctttg actttgtatc
     3721 gccattactc tgtagtattc tactcttgcc tcaaacttat catcattttg aatctttcta
     3781 gtaatccaat tcaaataacg attatagaat ctgattgcta ttaaggcctc atgtctgtta
     3841 tcttcatcag tatttttatt tataaatgat atagcagttt tagtagaaag tgcatatgca
     3901 tggtgaaact ctttgattat ttctttattc tcagatacta tttttgtaat tttttctttt
     3961 acttctttct ttacttttct atttataatc cacatgctta tttttaattt tacaatttta
     4021 aatatagatt tttgaacaga ttttcttgat tttgtaatat aaaatctata caatttaaag
     4081 gctctatcag aaattttacc atcttctaat agtttcaaaa cagctaataa tccctgttgg
     4141 tctgcttctg caatcatcat acttataagt ttattatctg gagtatttag tattcctttt
     4201 tctgagtcta ccaactgttc atttaaatca tgtataactg gttgtacttc ttgtgctttt
     4261 tcattactaa gttttaaaat ggcttctttc aaaattagtt tatatgcctc tttatcagat
     4321 aataaaggtt ctgactcttg tacgttattt tttaataatc ttggcagtaa aactgttgcc
     4381 aatactaaac ttataagaat aacttctgat gatataaata aaacagtatc tctcattgga
     4441 aatacatctt tatcagccaa ataaaaaggt atagataagg cagttgctag tgtaaatgta
     4501 ccatgaacac cacaagtagc aactattagt gagtatttac atttagaaat actctctttt
     4561 acaacttctt catccttaaa acctaaaaaa cctacaaaaa aattatttaa aggattcttt
     4621 ttaggtttta taaaagaatt atgccataaa tatacccata taaatcttat aacaaataaa
     4681 ataagtacaa ttaaagtaga aataaaagtc aattccaatg ccatattttc atttttacta
     4741 ctaagtcctg tatatatact tggtaacaaa aaaccaagca gtgtaaagac aaatccattc
     4801 aatacatact ccaaaacata ccaagtattg tctgatatta tccttaattt agtagttgta
     4861 ttttgtaaat gctctttttc aattccatga gctatccctg ccattacaac agctaggatt
     4921 ccagatactc ctatttcttc tgctacaaaa tatacaaaaa gtggagtcat tatttgtata
     4981 actattacca ttggaatttc ttccaaattc cacttgtgta aactaagtct tatttttata
     5041 attatgtatc ctaaaatact tcctactatt attcctccaa aagctgtaat cataaattga
     5101 atccctgcct cttctattga aaaaactcct gtaactgtgg caagtactgc aaccttaaat
     5161 gctactactc cagcagcatc atttaataaa gattcacctt caagtatact catcatattt
     5221 ttaggaaaat ttaatccttg tgtaattgat tttactgcta ctaaatcagt tggagatatt
     5281 actgctgcca aagcaaatga aacagataat ggcatcccag gaataagaaa atgtataaag
     5341 attccacctg caaacacggt tattaaaact aaaccaaatg caagaagcaa aattggtttt
     5401 ctgagctcta atagctcttt tctcgataca ttttgtcctt cactgaatag taatggagct
     5461 atgatacata ttacaaatat ttctggatta aatgaaaatg agaatttaaa tggcaaaata
     5521 gacaatataa tccctagtac aatttgatat attgcaagag gaatattact ccactttttg
     5581 ctaaatacag ttgaaatcgt tgtacaaacc aataatccca acaatgttac taaaattgac
     5641 ataactacct ctcttttcaa taataatatt atagattaaa cttatatatc aactaactta
     5701 atattaacag catgaaatcc catcttaatc aaaaaaattt tcagtttatt ctattattct
     5761 tattatatac atcaaattta atttttgaaa tatctatact agatgacatg caacaaaatg
     5821 atttcttgat atttctttta aaactggaat ctcttttttg catatttcct tacaataagg
     5881 gcatcttgta tgaaacttac atccacttgg tggatttgtt ggagatggta tgtctccttt
     5941 taatataatt ctatttttct taattgtagg gtcttgtatt ggtactgcgc tcataagcgc
     6001 ttttgtatat ggatgttgag aatgtaagta aagttctcct ttagttgcaa tttctattat
     6061 agaacctaga tacataacac aaactctatg tgcaatatga tttactacac tcaaatcatg
     6121 agatataaat aaatatgaaa gtttatattt atcctgtaaa tccataagta aatttataat
     6181 ttgagattga atagaaacat ctagtgcaga aacaggttca tcacaaacta taaattctgg
     6241 atttaatgct aatgctcttg ctatacctat tctctgtctt tgacctccag aaaactcacc
     6301 tggttttcta tttaaatggt acttagaaag accacaactt tctacaacat ttaatatatt
     6361 atcttttatt tcattctttg gcactaaatt atgttctata actgcctcgg atattatttg
     6421 ttccacattt aatcttggat ttaatgaact atatgggtct tgaaatatca tttgcatttt
     6481 gggtcttaac tttctaagtt cttccttctc taacttatat atatctacac cattaaattc
     6541 aactttacct tcagtttttt caattaatct aagaatggtt cttcctgcag ttgattttcc
     6601 acatcccgat tctccaacta tagccaaagt ttctcctttt tttaaatcaa aagaaattcc
     6661 atctactgcc tttacacatt taatttcctt atcaaataat ccagatttaa cttcaaagta
     6721 ctttttaaga ccttctactt ttaataaact ctcttctttt ttcaattata tctcctcctt
     6781 tttatataac caacatgcaa tactatgacc actattcaat tctattaaag gaggtatctg
     6841 agttctacat ttatcacata ctttttcaca tctatcacta aaataacatg aatctggcat
     6901 cccaattgga tttggtactt gtcctggaat cgaatacaat cttttatctt tgttttttcc
     6961 aagagcaggc tttgatttta gaagtccaat agtataagga tgttttggat ttttaaataa
     7021 ttcaacaacc tctgccactt ctataatttt gccagcatac atcactaata ctttgtcaca
     7081 catttctgca actactccca aatcatgtgt aatcatcatt atagatgtgt ttaatttttc
     7141 ttttatattt ttcattatat ctaaaatctg agcttgaata gtaacatcta aagctgttgt
     7201 tggttcatca gctataagta attttggatt acatgataaa gccatagcta tcattattcg
     7261 ttgtctcatt ccacctgata attcatgtgg atatgaatta tatatttctt cagctcttgg
     7321 tatttctacc agttttatca tatcgattac ctttttctta gcttcttcct ttgataaatc
     7381 ttgatgtatc ataatacctt caatgatttg acttccaata gtaaacgctg ggtttaaaga
     7441 tgtcattggt tcttgaaaaa tcattgatat ctcattccct cttattccca taagttcatc
     7501 ttcattcatc tttaaaatat ctttgcctcg aaagattatt tctccacctt ctatttttga
     7561 tgttggaagt aacttcatca aagacatggc tgttacagac ttaccacatc ctgattctcc
     7621 aactattcca atagtttcac cttcatttat attaaaactt acttcattta cactctttac
     7681 aatcccttta tctgtatgaa aataaacttt taaatcttta acctcaatta gtgctttact
     7741 cattattttc tcctctcagc aaaattactt aagataacat acaccttttg attgtatttt
     7801 ataaaaattt aaattacaac attttcttac attttattat ttttttctat taattataat
     7861 aatataatag ttaatttcta tatatattga tttaataatg attactatta tacaataaaa
     7921 aatatgtgtt aaatattatt ttatggaggt tttataagaa tatgaaattt aaaaaattgg
     7981 catcattaat cttagtttca tcattaatgc ttacatttac agcctgtgct tccaatgata
     8041 aagacaagtc tagtggaagc aaatctggtg gaactgttgt aatacctatg gcaagtgacc
     8101 cagatataat caatggagct tttgcaaacg ttaaagaaga ctctcttgca tcaaatatag
     8161 tttatgctcc tctttatact tatgaaaaag gaaatttagt aaattattta gcagaaaaag
     8221 tagattttaa agatagtaag gaattaacta taaaattaaa atccaattta aaatggcatg
     8281 atggaaagcc aataactgct gaagatgtct tatttacatt taatacagtt ttagacgaaa
     8341 aacaaaattc accatcaaga caatacttat tggtgggtga gaaaccagtt aaagtagaaa
     8401 aaattgatga tttaacagtt aagattactt taccaactgc aagtgaatct ttcttatatg
     8461 gtatatctaa aatttctcca ataccaaaac atgtgtttga aggagaatca aatatagcta
     8521 agtcagaaaa aaataataat ccagttggtt ctggtgcatt caagtttaag gaatggaaaa
     8581 aaggtgaaag catagtattt gaaaagaatg cagattattt tggtggagaa tctaaggcag
     8641 attctatagc gctaaaaata attcctaatg aagcatctca agaagcagct ttaaataatg
     8701 gcgaaatttc acttatgaag acatctgctg aaggatatga aaaggcaaaa tcaaactcaa
     8761 atttacaaac ttatacttat agtgaagaaa gattaaacta cattgtattt aaccaaaata
     8821 tatcaaacat ggctaacaaa gaagtaagac aagccttatc atatgcctta aatagaaatg
     8881 aaatgataga atcagcatat ggaaaagaag gttctgtacc tgctaaatct atattagttc
     8941 cagaagcaga cttctataca gaagaaggtg ttgaaggata tgaccaagat acaaataagg
     9001 ctaaagactt attggataaa agtggtgtta aaattgataa attaaaaata ggttataaca
     9061 caggtagatt tgggcataaa aactatgcct tagtagctca acaagagttg aaaaaaattg
     9121 gtatagaagc tgaaatagtt ccttatgaaa gtaaggcatt cttcaatata ctatttagta
     9181 atagcactga atgtgatatg tatgtaaatg gttatgcttg gggattagaa ccaaacccat
     9241 atagaggtat gtttgaaact ggtcaatatt gcaaccaaac taaatattct aatgctgaaa
     9301 tagatgcttt atgggaaaaa ggatttactg agttaaacaa ggaaaaacgt gaagaaatat
     9361 ataagcaaat acaacaggat atatctaagg atgcgcctat ttacactata gattatgaac
     9421 aaaatttaat ggcagctcaa aaaaatctaa aaggaataaa agatgctaaa ccatctcctg
     9481 caatattatt tgaagattgg tctaaactgt atgtagaata attgatgtaa aattttatat
     9541 tattaattag aaaaggagat gtctaaaaat aatattgttt tattatttta aaacgagaca
     9601 tctcttttaa ttaggagaat ttacttatgt taaaattaat attgaataga gtaaaagttt
     9661 taatacctat gttaatctta atatctatat tatcatttgg acttttagaa ttagctcctg
     9721 gtgacccagc agatgcatat ataaatcctt taatgtcttc acaggatatt gaaaatataa
     9781 gggttaatat ggggcttgat aaaccagcat atataagata tttaaactgg ttgaaaaaca
     9841 ctttaagtgg aaacttaggt atttcttata taaaccatat gcccgttaca gaacaaatta
     9901 tggaaaaaat gggaaataca tttattctta tgggtacttc tttgatattt tcaatattgg
     9961 tagccatacc tcttggaata tttttagctg taaataaaaa ttcaataact agtaaagtat
    10021 catctgtttt taattatata ggtgtttcta taccttcttt ttggataggt atgatactca
    10081 tttcaatttt ttcagttaaa cttaacattt tcccatcagg aggtatgcat actattggaa
    10141 atgatagtat tggagattta gcaaaacatt tagttttgcc tgtaataact ttaggtctat
    10201 ataatactgc tatttttaca aattatgtag aagctagtgt aaatgaacaa ttaaaaaaac
    10261 aatatgttat tacagcaaga gcaaaaggat tatcagaaaa agtcatacta tttaaacatg
    10321 tcttgaaaaa ttcactgaca tcattggtta caatcctagg catgagtata caaaaattag
    10381 tcactggtgc atttgttaca gaagtagttt tctcttggcc aggtatggga agattaatga
    10441 ttgattcaat tttttcaaga gattatactg taattatggc aataactatg ttatcagcac
    10501 tatttttaat tttaggtaat ttagttgctg atatactata cttattaatt gaccctaaaa
    10561 taaaatctag caaaggagga ttctagttgt gtttacaagt atcttaaaaa aattaaaaaa
    10621 agataaatcc tttgcttttg catgtattac tcttagcata ataatcatat taattatagc
    10681 ttcttttata ttaaatatag actctaatgc aattgatact acttctaaac ttcaagaacc
    10741 tagcttaaag catatctttg gtacagacga acttggtaga gattacttct ctagagctct
    10801 ttatggtgga aggatttcac ttagtgttgg tattttatca atgcttattt ctgtagtatt
    10861 tggtactgct gtaggtgtta ttagtggata tattggaggt aagctagacc aattcttgat
    10921 gagattagtg gatatgctta tgagtatacc aagtttctta gttttgattg tattaaatac
    10981 ttatctaaca cctaaagttt ccactattat actaattatt ggattcttaa gttggatgga
    11041 agtttcaaga attgtacgtg gcgaaacatt aaaaataaag gaaaatgaat actgtctagc
    11101 tgctaaagct actggtatca aaactagaaa tatactatta agacatatag ttttaaactt
    11161 aaaagagact attgtagttg ctggttcgtt aaatattgct aatgctatct taactgaatc
    11221 tgctcttagt tttttaggtc ttggaatcca attgcctctt gcatcatggg gtaatatgct
    11281 tcaagatgct caaagacata tttttgacaa gttatattta gctgtatttc caggattact
    11341 tatattttta acagtcatga gtttgactat aataagcaaa aaattttcta gtaatactta
    11401 acaaatttta tatagaaaat aaaaacagtg attatctaaa gatatacact gtttttattt
    11461 ttcgtttaaa aaaatacata ttttgtatat tataacttta aatactactc taccatgtta
    11521 tgcaaagcgt attattgctc tcaaacatat agatgaaaaa ctaatagttt attataataa
    11581 aaattgattt atttatttgt tattttaata acatttttaa gagttctttt tactaaagaa
    11641 atctataata aatttaaata catgttggtc tacatggcta taacgttctt ttttaggtct
    11701 acataatata actttccatg taataggatg attaaaaggc ttttctataa ttttgctatt
    11761 ttcaaaaaaa tcatgaactg gtgatggtaa aattgttata aaatcagaat ttgtggttga
    11821 tagcaacaaa aaatcccatg aaccagacat tatatatctt tttaacttaa cattttcatc
    11881 agtaaatttt tgcattagct tatgatgtat cataaaagta ggattaaata ttgcaagtaa
    11941 ctgctcattc aaatcgttcc aatctatctt atccttctta gcaagtggat gatttatatt
    12001 taaaaatgct gtaagttcat cttcttgaag cacatgttca gttactacac ttttatcaat
    12061 tttatgtgga tgtaacaata ctgcataatc taattcctgt aagataagca ttctatttaa
    12121 gtcatatgca cccttctcta ctatatcaaa ttctatatct ggattatttg caactaactg
    12181 tgcaactaca tctgaaaatg ctattccaag tataagtgga ggtattccta ttctaacttt
    12241 tcctttaaat tgaactgaat cttctcttaa atcttccatc atatttttgt attcatttat
    12301 gatatgttct gcatttacat aaaatctttc tcctgctggt gttaacccat ttaacctgcc
    12361 tttatacctt tcaaataaat atacatcttc actttcttca aaatttttta taatttgact
    12421 taatgctggt tgagatatgt gaagtgtctt agatgctatt gataaattaa aaccagattt
    12481 cactatttca acaaaatatt tcaactgttt tatatccatt ttaaaccccc ttccccaaaa
    12541 gtataattta gattatattt tttcttctaa tgatatttta taggaaaatc aacataattt
    12601 cattaatatt ttaacaaaat atttttctaa atctactata aatttttatc ctataaattt
    12661 ttcttataca ctatataaaa aattagactt agacaaaacg cttttttcgc cgtaatctat
    12721 aattagaaac aggaaaacct aaagcattag gaataaaagt aattgaaata cttaaaaaca
    12781 agcttgtatt aaaaaagtac tttattatag tgttccctaa tttctttatt cagaaaattt
    12841 ttaattatca ttcaagaaag gtaactatta gatatgaaag atgtagtaat cgtatcagca
    12901 gtcagaacac ctataggaag ttttggaggt gtatttaaaa atacttcagc tgttcaactt
    12961 ggaactatcg ctgttaaaga agcgatatca cgtgtaagac ttaatttatc tgaaatagat
    13021 gaagtaataa ttggaaatgt tcttcaaaca gggcttggac agaatgttgc tagacaaatt
    13081 gctataaatg caggtatacc aaattctgtt ccaagctaca cagtaaataa attatgtggt
    13141 tcagggctta aaagtgtcca gctagctgcc caatcaatca catctggtga aaatgatgtg
    13201 gtaattgcag gtggtactga aaatatgagc caagctcctt atatcgttcc aactgctcgc
    13261 tttggtagta aaatgggtga cataaccatg gtagatagta tgctaacaga tggccttatt
    13321 gatgctttca atcagtatca tatgggaata actgcagaaa atattgcaac taaatttgaa
    13381 tttactagag aaatgcaaga taaattggca ttagaaagtc aaaataaggc tgagaatgct
    13441 ataaaaaaca accgctttaa agaagaaatc gttccagtag atgttttaat acgtcgagga
    13501 aaaatagaaa ctattgataa agatgaatat cctaaattag gtatgacatt tgaaggttta
    13561 tctaaactta aacctgcatt caaaaaagat ggtactgtaa cagctggaaa tgcctcaggt
    13621 attaatgatg gtgcggctat gttaatacta atgagccaac aaaaagcaga cgaactaggt
    13681 attagaccac ttgctaaaat aaaatcctat gcttcagctg gtgttgaacc tgaagtcatg
    13741 ggtactggtc caatccctgc aactagaaaa gcactgaaaa aagctggact tagcatcaat
    13801 gatatagact taatagaagc aaatgaagcg tttgctgccc aagcattagc tgttaaaaat
    13861 gaacttcaaa tagattcatc aaagttaaat gttaatggtg gtgcaatagc attaggacat
    13921 ccaataggag caagtggagc tagaatactt gttaccttaa tttatgaaat gcaaaaacgt
    13981 aaagttgaaa ctggacttgc aacactttgt attggtggtg gtcaaggaat atcaatggtg
    14041 gtttcacgat aggaggaaat atgaataaaa tagttagcat tgatgaagct ctatctcatg
    14101 taaaagatgg agatacaata atggttggtg gattcatggc caatggttca ccagaaaatt
    14161 taattgatta tttatgtagt aaaaatataa aagatttgac actaatttgc aatgatactg
    14221 gctttattga caaaggagtt ggcaaaatgg ttgtaaataa gcaatttaaa aagataattg
    14281 cttctcatgt aggattaaat aaagaaactg gaagacaaat gaataacaat gaaacagatg
    14341 ttgagttagt tcctcaggga actcttgctg aacaaatacg tgcagctggt tatggtctag
    14401 gaggtatact cacaccgact ggtattggta ctcttgttga ggagggaaag caaaaaattg
    14461 ttgtcgatag caaagaatac cttcttgaaa aaccaattta tgcagatgtt gctcttctat
    14521 ttgcatcaaa ggtcgataaa gcaggtaatc tagtttataa aggctctatg aacaatttta
    14581 ataatttaat ggcatctgct gccaaaatta caatagttga agcagatgaa atcgttgaaa
    14641 ttggtgatat tgacccaaat gaagttaata caccaggtat ttttgtgaat tacatcgttg
    14701 aaggaggtaa tttataatgg ataaattaga aatgcaagaa tatattgcaa atagagtatc
    14761 taaagaatta aaagacggag ccgttgtaaa ccttggaata ggtctaccaa ccaaagtagc
    14821 aaattatatt ccggataatg taaacgttat cttacaatct gaaaatggct ttttaggttt
    14881 aggttcagcc gaagatggaa attcaagtga tgaaactatt gtaaatgcag gtggtcaacc
    14941 agttactata cttcctggag gatgtttctt tgatagtgct acttcttttg gaataatcag
    15001 aggaggtcat gttgatataa ctgttcttgg cgcacttcag gttgacaaat atggcaatat
    15061 agcaaactat atgattccag gtaagatggt tccaggtatg ggtggagcta tggacctagt
    15121 aactggagct aaaaaagtta ttgtagcaat ggaacatact tcaaaaggtt ctgcaaaaat
    15181 attaaataat tgtactcttc cacttactgc aacaaatgct gtagatttga tagttactga
    15241 aatgggagtt atggaagtaa catcagatgg aattttactt aaagaaataa atccagcttt
    15301 cacattagat gatgttattt ctgctactga agctccttta atattgtcag attcattaaa
    15361 tggaaaaata tctgttgttt aaaataatta aaactagaaa ataaaaatct cttagacgta
    15421 ttacatataa atttgtattg cttaaaatat taatataaaa ataaaataaa attggaggaa
    15481 atgaaaatgg ttaaagataa agttgtattc gtaacaggag ctgcaagtgg aataggtaaa
    15541 caaatagctg aatcgttctt aaaaaatggt gctaaagtaa tgttctctga tataaatcaa
    15601 gaagctttaa ataataccat aacagagtta caaaaaaaag gttatgattg catgagtgta
    15661 aagtgtgatg taactaagga agaagaaatt aaccacgcta tagacaaaac tgttgaaaag
    15721 tatggcagat tagatatatt aataaacaat gctggacttc aacatgtttc tatgatagaa
    15781 gatttcccaa ctgataaatt tgaatttatg ataaaaataa tgctgacagc accttttata
    15841 gctactaaaa aagcatttcc tataatgaaa aaacaaggct ttggtcgtat aataaatatg
    15901 gcctcaatca atggtgtaat aggatttgct ggaaaggctg cttataactc agcaaaacat
    15961 ggacttattg gactaacaaa agttgctgct cttgaagctg ccgattctgg tataacagtt
    16021 aatgctttat gccctggata tgtagatact ccacttgtca gaggtcagtt caatgacctt
    16081 tcaaaaactc gtaatatacc attggaaaat gtcttagagg aagttttata cccacttgta
    16141 ccacaaaaac gcctattaga tgttcaagaa attgccgact atgctatgtt cttagcaagt
    16201 gataatgcta agggtattac aggacaagct tgcttactag atggaggata tactgcacaa
    16261 tagttattat atttagggct tgaagaaagt attcttcagg tccttctact aaggagggat
    16321 atttatgagt attatcgcgc caattggtat aattattggt atcatagcta ttatatattt
    16381 ttcagttaaa gaaattcata taactatagc tgctccaata gcaactttaa tagttgttat
    16441 tttaaataaa atggatgttg taacgtcaat gctaggtgct gacaaaaaca attatatggg
    16501 tgcacttgga aactatataa tgagttattt tgcaatattc ttattaggct caatacttgc
    16561 taaatttatg gaagaaagtg gagctactgt atcaatagct gattttattt taaataaatt
    16621 tggctctaat catccttata gagttttagt tgctatattt atagtaagtt ttatattaac
    16681 gtatggtggt ataagcttat ttgtagttat gtttgcggtc attccacttg caagaactct
    16741 atttaaaaaa cttgatattt cttggaacct tattcagata ccactttggc ttggtattgc
    16801 aacaattact atgactataa ttcctggaac accagccatt caaaatgtta tacctattca
    16861 atatctaaat acgtctctta ctgcagctac tataccaagt attgtaggta gtataggttg
    16921 tgctacattt ggacttgtct atatgaaata tgctctaaac aaaagtatta gaaaaggtga
    16981 aacttatgca acttacactt cagaattaga agaagaatct ttagatagag aattaccagg
    17041 attttttgca agtatttttc ctctattagc actcgtaatt atagctataa ctggaagtac
    17101 ttttggttca gaatttatga aaaaaaatgt aatttacata gctttaatag ttgcaattct
    17161 tttatctgct tttttattcc ataaatttct atcatctaag atacagacat tgagcatagg
    17221 tgctagtggt tctataggtc ctatatttgc tacatcttct gcagttgcat ttggagcagt
    17281 aataatggct gcacctggtt ttgatgtatt tagcaaactt ataatgtcaa taccaggtag
    17341 tccacttata agtcttactg ttttgacttc aacaatgtca gcaataacag ggtcctcttc
    17401 tggtgcatta ggaatagtaa tgccaaactt tgcagattat tatttaagta ctggtctaaa
    17461 tccagaatta attcaccgtg ttgctacaat agcttcaaat atactaacta tagttccaca
    17521 aagtggtgtt tttcttacat tcttaagctt atctaagtta acacataaaa ctggatttaa
    17581 agaaactttc attgtagttg cagttggttc actaatagca gaaattattg taattacatt
    17641 aggattacta atgtattaat ataaaacaag ttaaaattca taatatgaaa ggtggtgtct
    17701 caaagtgaac tttttactta atgagacact ctttttatat gaaataacat atgcctatca
    17761 tttaaacaat aaaaaaggcc ttatctctat tttgagacag acccttttta tttcttaaag
    17821 atttaaataa ttataatttg ttgctaatac gaaagcatct actgaataaa tttaaccaaa
    17881 taaaaaaaaa tttatcataa ctataaattt acgttatgga attattataa aaatattatt
    17941 ttctctaact aagtaattcc atactataca ggcttttaat acaaattgtc aaacacactg
    18001 aaatatacct atttattcca tatatatctt ttttacatat ctttacagta aaaaaagtct
    18061 taatcttatg ttagtataga ttcacgggta accccctaca ctaaaggtta caaatcagta
    18121 acatttttag aatttttaga ttaagaggtg tttaatgatg agaagaaata taccaacaaa
    18181 attaaaaact atattagctt taggagtgtt aactttagca acagcttcaa gtgctgttac
    18241 tgcaagtgct tcatcacttg aaaataatgt aaatataagt aacaaaagtt tagtatatag
    18301 taatactaaa tacaagtttt ctgcaaaacc aaattgttct gtaaaaccag aacttaagcc
    18361 tgataaagat aactcttgta aggataagaa tcataatgac aagaattgca acaacactaa
    18421 taaacctgag gacaacaata attcagactc tactaataag cctgataaca ataaacctga
    18481 gtctaataaa cctgaagaca acaataattc aggctctact aataaaccta ataataataa
    18541 acctgagtct aataaacctg aagacaacaa taattcaggc tctactgata aacctgataa
    18601 caataaacct gagtctaata aacctgaaga caacaataac aataattcag actctactaa
    18661 caaacctgat gataataaca actcaggctc tacaagtgaa agtttttcag cttaccaaaa
    18721 agaagttgtt gacttagtaa atatagaaag agctaaagct ggattaaatc ctcttactct
    18781 tgattcttct atatcaaatg ttgctacaaa aaaatctcaa gatatgattg ataacaacta
    18841 tttttcacac aattctccaa cttatggttc tccatttgac atgttgaaaa aatttggaat
    18901 aagctataag acagcaggtg aaaatatagc tatgggtcaa aaaactccta aagaagttgt
    18961 aaacgcatgg atgaattctg aaggtcacag aaaaaatata atgaatccaa atttcagtaa
    19021 aataggtgtt ggagtagcac aaaaaagtgg tggttcaatt tattggacac aaatctttgt
    19081 aggataaaat actctttttc catacatatc aaaagagtat ctctaagtga taaaaaacac
    19141 aaatgagata ctcttttgta tctattttaa aattatcaaa tccaaatata catactataa
    19201 atattatttg ttctcttaat tttagtgtgt agaacaatat ataaaaagta ttggcaagat
    19261 gaaaatggaa aacttaaaaa agatatttaa taggtaaaaa caaaatctta atatcttcat
    19321 aaaaaaaggc tgtctcaaaa tagagataag gcctttttca ttgttgaaat gattaaaata
    19381 tatttgattt catacaaaaa gagtgcctca tgaactaaaa agctcacttt gagacactct
    19441 cttttatcaa tttatataag cattataata ctttaatatt attcagctaa ttttttgtat
    19501 ccttcatatc tttctttagc atcagcttca gctttagcaa ataatccatc agcttcttct
    19561 gggaattgtt tagctatagc agaatatctt acttgtgcaa gtatgaaatc tttgaagctt
    19621 gcagttggtt cttttgaatc taaagtgaat gggtttttac cttcagcttt tacttctgga
    19681 ttgaatctgt ataaatgcca gtatccgcaa gcaacagctt gtgcttcgtt agctacagtt
    19741 cttcccatac cttcttttaa tccatggttt atacatggag cataagctat tattagagat
    19801 ggcccatcat gtttttcagc ttctataacg gctttgataa attggttttt atcagcaccc
    19861 atagcaactt gagctacata tacgtttcca tatgtcatag ccatcattcc taaatctttc
    19921 tttctagatt ttttacctga agcagcaaac ttagccatag cagcagctgg agtagcttta
    19981 gaagcttgac ctcctgtatt tgaatatact tctgtatcaa atacaagtac gtttacatct
    20041 tcaccagatg caagaacatg gtctaatcca ccgtaaccga tatcatatgc ccaaccgtct
    20101 ccacctagta tccattgaga tctcttaact agataatctt ttctatcttt tatagctttt
    20161 actaactctt tagctttttc atctgtacaa tcacaagctg gtttttctaa taattcaaca
    20221 accttagcac ttgctacttt agaagcttct ccatcatttc tagaatctaa ccactctgta
    20281 aatactgctt tagcatcttc acagataccc atagaaacta actctgtcat agcatctact
    20341 attttatttc ttatttgctt aacagctgtg tacataccaa atccatattc agcattatcc
    20401 tcgaataaag agtttgccca agatggacct ttaccttcat ggttacaagt ataaggaatt
    20461 gatggtgcag aagctcccca tatagaagaa catccagtag cattagctat catcattcta
    20521 tctccatata attgtgtaac tactttaatg tatgcagttt ctccacatcc agcacatgct
    20581 cctgaaaact ccattaatgg ttgtctaaat tgacttcctt taacagtgtt tacaggcata
    20641 acatcacctt ttggagctac ctcatcagga tttactgcaa atgcccaatt atctaactct
    20701 gtatcaagtt gagtatctat tggcttcata actaaagctt tttctttagc tggacatatg
    20761 tcagcacagt ttccacatcc tgtacagtca agtggactaa gttgcattct atattttaat
    20821 ccttctaatc ctttacctaa tgctttttta gcttcaaatc cttctggagc attagctaat
    20881 tcttcttcag taactaatac tggtcttata catgaatgag gacatataaa agaacattgg
    20941 ttacattgaa tacagttatc tattatccat tcaggaacat caacagctat accacgtttt
    21001 tcataagctg cagtaccaca agggaatgtt ccatcttcta taccattgaa tgtacttact
    21061 ggtaagttgt ttccttcttg tgcagtcatt ggtcttagta tattttttat aaattctggt
    21121 tctttaactt ctcctttatc tgcatcaaca gcattttccc aagaagctgg aacttcaact
    21181 ttaactaaag catttattcc tgcatcaaca gcagcatagt tcatgttaac tattttttca
    21241 ccttttttac cataagcttt aactatagaa tcttttaagt atttaacagc atcttcttct
    21301 ggtattattt cagctaattt aaagaatgca gattgcatta tcatatttat tctgtttcca
    21361 agacctattt cttgtcctag tttaacagcg ttaactgtat agaattttat atcattttta
    21421 gctatatatt gcttcatatg agctggtaaa tgttcttcaa gtcctgcttg gtcccatata
    21481 gtgttaagta caaatgtacc acctttttta agacctttta ataaatcata ttgataaaca
    21541 tatgattgtt tatgacaggc aatataatca gcttcatcta ttaaataagt agctcttatt
    21601 ggagtatcac caaatcttaa gtgagacata gttataccac cagatttttt agagtcataa
    21661 tcaaagtaag cttgagcata tttttcagta tggtcaccaa ttattttgat agcttgttta
    21721 ttagctccaa cagtaccgtc agaacctaat ccccagaact tacatcttat agttccttta
    21781 gaagctattt ttattggctc agatggagtt aaagaagtat tagttacatc atcaactata
    21841 cctactgtaa atccattttt aggttgttct gaaactaagt tatcaaaaac agtttttata
    21901 tctgaaggag tagtatcttt tgaacctaat ccgtatcttc cacctattat cataggagca
    21961 ttttcttttc catagaatac atcacgtaca tctaagtata atggttcacc agttgaacct
    22021 ggctctttag ttctatcaag tacacatatt ctttcaacag tagatggcat agcatctaag
    22081 aaatgtttca ttgagaatgg tctatataaa tgaactttaa ctaaaccata tttttctccc
    22141 ttagcattta agtaatctat agtttcttct attgtttctg ttactgaacc catagctatt
    22201 aatacgtatt tagcatcttc tgctccatag taatcaaata aactatgttt tctacctgtt
    22261 aaattactca ttttttccat gtatttttca actataccta ctatattttg atagtattta
    22321 ttagaagctt ctctagtttg gaaatatata tctgggtttt gagcagtacc acgagcaact
    22381 ggatgatttg gagataaagc attatctcta aatgcttgaa ctgcttcaaa gtttaataaa
    22441 cttgcataat cttcattttc tagtaattca actttttgga tttcatgtga agttctgaat
    22501 ccatcaaaga aatgtataaa tggtaatcta ccttctattg cagctaaatg tgcaacaggt
    22561 gctatatcag ctacttcttg aactgaacca gaagctaata atacacatcc agtttgtcta
    22621 gcagccataa cgtcttggtg atccccaaat attgataacg cttgagatgc taaagcacga
    22681 gcacttacat ggaatactcc tggtaataac tctcctgcaa ctttatacat gttaggtatc
    22741 attaataata aaccttgaga tgctgtaaaa gtagaagtta aagctcctcc ttgtaaagaa
    22801 ccgtggaatg ttcctgatgc acctgcttct gactgcattt caacaacatt aactgtttgt
    22861 ccaaatatat tttttcttcc ttgtgatgcc cactcatcaa ctacttcagc catagttgaa
    22921 gatggtgtga ttggatatat agctgctaca tctgtaaacg cataggcaac atgagctgca
    22981 gctgtatttc catcaagtgt tttcataaac ttagccataa tatgtatcct ccttgatagc
    23041 tacaattgta cttgtttata acaagtctat ttttttatat ttacatataa tttacacaac
    23101 aaaactataa acacacatat agtcgcgcaa atactctatc ttctaatata atactacaga
    23161 ttagataaaa ttcctttgaa aattgtaaat taattacata tttaagtaga ttttttttaa
    23221 aattaaatat gttaagtttt tttcataatt ttgtatacaa tatataataa attaagttag
    23281 atttttcaaa cttttttatc tatttgttca tatta
//
LOCUS       DAYATN010000050        23266 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.050, whole genome shotgun sequence.
ACCESSION   DAYATN010000050 DAYATN010000000
VERSION     DAYATN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.050"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            451..1521
                     /locus_tag="ACGI8A_001876"
     CDS             451..1521
                     /locus_tag="ACGI8A_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179873.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1505..2374
                     /locus_tag="ACGI8A_001877"
     CDS             1505..2374
                     /locus_tag="ACGI8A_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFH0179874.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2418..3278
                     /locus_tag="ACGI8A_001878"
     CDS             2418..3278
                     /locus_tag="ACGI8A_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFH0179875.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3309..4613
                     /locus_tag="ACGI8A_001879"
     CDS             3309..4613
                     /locus_tag="ACGI8A_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0179876.1"
                     /translation="MKLKRKLATGLAITLLLCSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4676..5938
                     /locus_tag="ACGI8A_001880"
     CDS             4676..5938
                     /locus_tag="ACGI8A_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFH0179877.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6125..8095
                     /gene="uvrB"
                     /locus_tag="ACGI8A_001881"
     CDS             6125..8095
                     /gene="uvrB"
                     /locus_tag="ACGI8A_001881"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFH0179878.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8114..10939
                     /gene="uvrA"
                     /locus_tag="ACGI8A_001882"
     CDS             8114..10939
                     /gene="uvrA"
                     /locus_tag="ACGI8A_001882"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFH0179879.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11198..13015
                     /gene="uvrC"
                     /locus_tag="ACGI8A_001883"
     CDS             11198..13015
                     /gene="uvrC"
                     /locus_tag="ACGI8A_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFH0179880.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13015..13944
                     /gene="hprK"
                     /locus_tag="ACGI8A_001884"
     CDS             13015..13944
                     /gene="hprK"
                     /locus_tag="ACGI8A_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFH0179881.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14156..15952
                     /locus_tag="ACGI8A_001885"
     CDS             14156..15952
                     /locus_tag="ACGI8A_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HFH0179882.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENIILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16092..17873)
                     /locus_tag="ACGI8A_001886"
     CDS             complement(16092..17873)
                     /locus_tag="ACGI8A_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HFH0179883.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17896..19782)
                     /locus_tag="ACGI8A_001887"
     CDS             complement(17896..19782)
                     /locus_tag="ACGI8A_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HFH0179884.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19799..20296)
                     /locus_tag="ACGI8A_001888"
     CDS             complement(19799..20296)
                     /locus_tag="ACGI8A_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HFH0179885.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20614..22095)
                     /gene="cls"
                     /locus_tag="ACGI8A_001889"
     CDS             complement(20614..22095)
                     /gene="cls"
                     /locus_tag="ACGI8A_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFH0179886.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            22413..23183
                     /locus_tag="ACGI8A_001890"
     CDS             22413..23183
                     /locus_tag="ACGI8A_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFH0179887.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         8910 a         3092 c         3899 g         7365 t
ORIGIN      
        1 ttttttgggt aaatttataa aaattaaaaa atttttattt tttatgttta aaccttgtaa
       61 atttgggaca taatataatt gtaaaactta atatagcaaa aataaattac tcaatctccc
      121 ccacttaaag acctctatcc cccatagagg tcttttttac tttcaaacca aattatataa
      181 aaaataaata gttttatatg actcttcata aattaaaatt acattgtgat agcaaaccat
      241 gaatatgaaa aatatagaaa attatagcta acataagctg tatatcatct taacttcagt
      301 atatttaaca aaaaatgttt ataatttaac ctttatttta ataagtttac catatattca
      361 ctttgtctta atatatcctt tacaaattaa gagtattatt aatctataag taaatcgtag
      421 aattaaatta aatattttgg gaggataaaa atggcagata taagactaga aaatgtaaca
      481 aaatcttata ataaaaaaaa tacagttata gaaaatttaa atcttactat agaagatggt
      541 tcttttacag tattggtagg cccaagtgga tgtggtaaat ctactacgtt aagaatgatt
      601 gcaggtcttg aagatataac ttcaggtagg ttgaagattg aagaaaatga tgttacaaaa
      661 acggatgcta gtaaaaggga tatagctatg gtatttcaaa attatgcatt atatccacat
      721 atgactgtta gagaaaacat agaatttggt cttaaaaaca aaaaagtaga aaaagaaaaa
      781 agagaaaaac ttataacaga agttattgat gtagtaggat tgagagaata tttaaatgtg
      841 aaaccaggca acttatctgg aggtcaaaga caaagagtgg cacttgctag agctatggtt
      901 aaaaatccaa aggtattttt aatggatgaa ccattgtcaa atttagatgc taaattaaga
      961 aatcaaatga gaacagaatt aatacaatta catcaaaaac taaagtctac atttgtttat
     1021 gtaactcatg accaggttga ggcaatgtca atggcagata agatagttat aatgaacaaa
     1081 ggaaagataa tgcaaatagg ttctccaaag aagatatatc aaaatcctaa aaatttattt
     1141 gtagctcaat ttatagggag tccttgtatg aacatactag atataaaaaa tgatattaaa
     1201 tttggattca gaccagaaaa agcaacacta gagagtataa acatagatgg gaaaaatgat
     1261 gatgtattta gtctatttgg aaaagttgta actaaagaag tgttgggaaa tgaaactata
     1321 tattgtttat ctgttcttaa tagagagata agagtcaaaa cggaaaatga tatatttgat
     1381 attggaagaa ctttaagaat aagtgtatta gaaaaagatt tatattattt tgataaaaat
     1441 ggagaaagaa tagaagatgt aaaagtatgt aaaagtatgt attctaaaat caaaggagat
     1501 atttatgaaa aagcaattta ataaagcata tttatatata acacctgcta ttataggaac
     1561 tttattgttt atagtctatc caataattaa gacgatatct ctaagcttca aatctggtag
     1621 tcttcttagt tcaacctttg aaaatgtagg cttaaacaat tatacagaac ttttaaagaa
     1681 tccagagttt atacaaacta taacaaacac tgcaatttat acttttttta tggtgactat
     1741 atctatatca ctagccatag tacttgctgt ttggctaaat aaaaattcta taatacacaa
     1801 tttaacgcaa agtataatct ttacaccaca tgtaatttca ctagtatctg tagctgtatt
     1861 gtggctttgg attatggagc cacaatatgg ctttttaaac tggatactat cgacattaca
     1921 tttaccaaca tcaaagtggc tttcaagtga aagttcagct ttaatgtcac taattctaat
     1981 aggaatatgg aagactcttg gatataatat cttaatagtg ttatcaggtc ttaaatccat
     2041 accaaactat atatatgaag catctaaatt agataatgca aacaaaataa cgaatttctt
     2101 taaagtgaca ttgccactat tatcaccaac aatatttttt cttatgataa caacaacaac
     2161 tgcatctttt caagtatttg atgagataag tataatgaca caaggtgggc cagtgggttc
     2221 aactaacatg ttatcttatt atatatatga gtctggattt atatactatg acatagggca
     2281 agcgagtgca gcgagtatgt ttttgttagc tatagttatg attgctacat atgctaactt
     2341 tagactctta tcaaagaaag ttcattatca ataaagaaaa agtagatatt ataaggttta
     2401 tccaaaagga ggagacaatg gatattatac aacctatcaa aaaagacaaa gttgatataa
     2461 aaaaaatagc atcaaaatca atagaaatat taattctatt acttttatct gcaatattta
     2521 tattcccttt tatatggatg ctatctacat cctttaaaaa tccacaagaa atgatgcaat
     2581 taccgcccac tattatacca aatgatatag tgttagaaaa ttatagtaca gcatggcatt
     2641 caggtccatt ttttagatat ttgctaaaca gtgtatttgt aacaggaagt gttttaattg
     2701 tacaattttt agttatggtt ccagcagcat atgcattttc taagattaaa tttaaaggtg
     2761 aaaaaatgct atttgggctt ttgcttgtag gacttatgat tccaccacaa gtaacttttc
     2821 taccagtcta tatgatgatg agtaagtttg gatttataaa tacttatgtt ccacttataa
     2881 ttccatttat gacatcttca tttgggatat ttctgcttag acagaatttt atgcagattt
     2941 cagatgaaat tattgaagca gctaaactag ataatgcttc tgacttaaaa ataatgttta
     3001 gaataatggt tcctatggca aaaccagcag taataacatt tatattattt aattttatat
     3061 accattggaa taattatttc tggcccttag taatgacaaa tacagatgca ataagaactt
     3121 taccaatagg agttgcgctc cttaaatctt cagaaggaat tactgcatgg aatgtaataa
     3181 tggcaggaaa tataattcta attttaccaa taattcttgt ttatatattt gcgaataaaa
     3241 aagtaaaaga agcttttatg tattcaggaa taaaataaaa aattaaagac taaatttgga
     3301 ggatgaaaat gaagcttaaa agaaaattag caacaggatt agcaataaca ttattattat
     3361 gttctttagt tgggtgttca aattctaatg atgcaaatgc atctaaagga gaaaagactg
     3421 atagcaatat tacgaagata gatttctggg ctcctttagg tggaactaat ggagaaactg
     3481 ctcaaaaaat ggttgaccaa tttaatagtg aacataagga tgtacaagta aatatgttaa
     3541 aacagaaaga ttactatgaa aatgcaacaa aactacaggc agccttaaca tctaaagacc
     3601 aaccagatgt aacattatta gaaataactc aaacaggtac attcgcatca gcaggtgctt
     3661 tagtagatat gagtaaatat tttgataaaa catatcaaga aagattcttt tctggtcttt
     3721 taacaaattc atattatgag gataagtttg ttggtatgcc atttaataga agtacaccaa
     3781 tattatacat aaataaagat atggcaacta aagcaggtct tgacccttca ggtccaaaat
     3841 catgggaaga gcttaaggat tatgctagta aaatgacaaa taaatcagaa gatacttatg
     3901 gttttgaaac accaatagat atttggttct atgaagctat ggtaatgcaa agtggaggag
     3961 aaataactaa tggtaaaaaa gtagcattta ataatgaggc aggacaagca ccagttaagt
     4021 tctggcaaga tatgatgaaa gctggaataa tgaaaatgcc tcctggagaa gattataatg
     4081 cctgggacgt agcaaaacaa gattttgtaa atggtaaagt aggtatgata tttacatcaa
     4141 cagcagattt agcaggactt atgcaacaaa cagaaggtaa gtttgaaata tcaacagcat
     4201 tcttaccaaa aaatcaaaaa tacgcaactc ctacaggtgg agctaatcta gttatgcttg
     4261 aaggtggaac agataaagaa aaagaagcaa gtgcagaatt tatagaatgg atgacacaaa
     4321 cagataaaat cgttcagttt agttcatcaa caggatactt accaacaact gaagatgcaa
     4381 caaactcaga aaagttacaa acattatata aagaaaaacc tcaatataag gttgcaactg
     4441 accaattaca atatgctgtt gcacttccaa tgttaaatgg atacaaggaa gcaactgata
     4501 agctaatgga tgagattaaa aaggggctta ctgatttaaa tgcaaatcca aaagatgtag
     4561 taagcaaagg aacttctgca atgcaagcag taatagataa aacaaataaa taattatata
     4621 gacttgtaag ttgattaaaa gtattcaata atatataaat gaagaggtaa tgtaaatgac
     4681 aaaggttata gtattatcag tagattcttt atttgaaaaa gacttggaat ttgtaaaaaa
     4741 tctaccaaac tttaaatcta tactagaaaa ttgctccata gtaaaaaata taaattgtgt
     4801 ttatcccact ctgacatatc cgtgtcatac tagtatggtt acaggagtat atcctaaaaa
     4861 acatggtata tatcataatg aaaaatttga cccaaataaa gaaaataaag actggtactg
     4921 gtactcaaag gacataaaag caaaaactat tatagatgta gcaaaagaaa ataatctaac
     4981 tacctctagt gtattatggc ctgtaatggg agcaaatcca aatatagatt ataatattgc
     5041 agaaatatgg gctcctagta gagaagatga cccaagagaa acttttgaac gaagttccag
     5101 taaggttata atggacggta tttacaatag acattgtcat tatatcgatt ggaaattaga
     5161 acccaacatg gatttatttg gagtaaactg ttcagtagat ataataaagg aatataaacc
     5221 agatttaatg ctaatacatt tagctacatt ggaccataca cgacacaatt atggcttatt
     5281 taatgacgaa gtagataaag cattaaaaat gaatgataaa tggctaggag atattattca
     5341 ggctacaaag gatgcaggta catacaaaga tacaaatttt ataatacttg gggaccatgg
     5401 tcatcttaga gttgataaag ttataaatcc aaatgtcttg cttaaatcaa atggatttat
     5461 aaaagttgaa aatggagaag ttaaggattt tgatgcctat gtaaattctt ctggaatatc
     5521 agctcaaata atagtaaata acttagatag gttaacagag ttaagagaac tgcttgaaga
     5581 atttaaagaa gaattattca tagagaatat ttttaccaaa gaggaagcat ctaaccttgg
     5641 tcttgaaggt gattttgaaa tggtaattga aggtcttgaa ggaatttcat ttggaaatga
     5701 tttcgaagga agtattataa aagattcaga tataaaagat tacaagtttg ctgttgcgac
     5761 acatggtcat ttacctacta aaggtaatag acctccattt atagcttttg gaccaaatat
     5821 taaaggtggt gtggttatag aagaaggaga cctgagagca catgcagcaa ctatattaaa
     5881 aatgtttaat ttaaaattag atggagttga aaaagaagca tttgatttta ttatgtaagt
     5941 tgaataaagt atttattttc catgaattat agacttctat tgcaaagata gaagtctttt
     6001 tttatgtttt agatttaagt aaaatttaaa tgggtaaaat atttaaaata acttaaacaa
     6061 ttttataaag gtatgatata ataatacatg tcaggaacta atgttcgaaa aaaggagtga
     6121 tactatggat tttaaaataa aatcagactt caaacctaca ggtgaccaac cagaagctat
     6181 taaatcaata gtggattcta taaatagaaa tgagaaattt tcgacattat taggtgtaac
     6241 gggttcagga aaaacattca caatggcaaa tatcattcaa caagttaaga aacctaccct
     6301 aataatggct cataataaaa ctctagcagc acaactttac agtgagttta aagaattttt
     6361 cccagacaat gcagttgaat actttgtaag ttactatgat tattatcaac cagaagcata
     6421 tgtagctcac agtgacactt atatagaaaa agatgcaagc ataaatgatg aaatagataa
     6481 acttcgacat tctgctacag catctatttt agagagaaga gatactataa taatatcatc
     6541 tgtatcttgt atatatggac ttggtgaccc aaaagactat aaagaactta tgttatctat
     6601 aagaccaggt atgcaaagag acagagatga tgtaataaaa aggttaatag agatacaata
     6661 cgaaagaaat gacataaact ttacacgagg tacctttaga gttagaggag atatactaga
     6721 aatattccca gctagtaatg atgaaaaagc aataagaata gaattttttg gtgatgaggt
     6781 agatagaata acagaaattg attatgtaac agggaagatt gttggtacta gaaatcatgt
     6841 tgttatattc ccagcatctc actatgtaac aacgccagaa agaattgaaa aggcaattgt
     6901 agaaatagaa aacgagcttc aagaacaaat taaatttttc aaggaaaatg ataggctatt
     6961 agaagcacaa agaattgaac aaagaactaa gtatgatata gaaatgttaa aggaaatagg
     7021 attttgtcag gggatagaga actattctag acatattaca ggaaggtcag aaggagaaag
     7081 accatatact ttaatggatt tcttcccaga tgattatttg ataattgtag atgaggcaca
     7141 tgtcactata ccacaagtaa gaggtatgta tgcaggagac aggtctagaa aaacatcact
     7201 tatagagaat ggttttagat taccttctgc acttgacaat agaccattaa attttcaaga
     7261 atttgaagga aatataaatc aaatgttgtt tgtaagtgct acaccagggc cttatgaaat
     7321 tcaacactca gagacaatag cagaacagat aataagacca actggccttt tagacccaat
     7381 tgtagaagta agacctataa ataatcaaat agatgatttg gtaggagaaa taactaaaac
     7441 tatagaaaaa aatgaaagag tgcttataac cactttaact aaaaaaatga gtgaggactt
     7501 gactaactat cttaaagaaa tagggataaa agttaaatat ttacactcag atatagttac
     7561 attagaaaga acagaaataa taagagattt aagattaggg aagtttgatg tccttgtagg
     7621 aataaactta cttagagaag gattggatat accagaagtt tcacttatag caattttaga
     7681 tgcagacaaa gaaggtttct tacgttccga aacagcttta atacagacta ttggacgtgc
     7741 agctagaaat gaaaatggaa gagtaattat gtatgcagac aggataactg actcaatgca
     7801 aaatgctatt gatgaaacaa agagaagaag agatatacaa aatttatata atgaagagca
     7861 caatatcatt cctaaaacta tacagaaaaa tataagagat agtatagaag ctactaaggt
     7921 tgcagaggaa gaagttgtat atggaatatc tgatacagat gataaagacg aaataagagc
     7981 taatatagat aaattaaaat cagagatgat ggaagctgct caaaacttac aatttgaaag
     8041 agcagcagaa cttagagata aagtgaaaca attagaagaa aaattggaaa aatagaaagg
     8101 taaaaacaac attatggaag ataaaattat aataagagga gcaaaagaac ataatttaaa
     8161 aaatatagat ttagaattac caagagataa atttattgtt tttacaggtt tgtcagggtc
     8221 tgggaagtct tctttggcat ttgatacaat atatgcagaa ggacagagaa gatatgtaga
     8281 aagtctatct gcttatgcaa gacaattttt aggtcagatg gaaaagccaa atgtagaata
     8341 tatagagggt ttatcaccag ctatctcaat tgaccagaag actacctcaa aaaacccacg
     8401 ttctacggtt ggaacagtaa cagaaatata tgattatttg agactattat ttgcaagagt
     8461 aggagatgtg tattgcccta catgtggtga aaaaataagt cagatgacta ttcaagaaat
     8521 agttgataaa atgctagatt ttccagatag aacgaaatta caaattcttt caccaattgt
     8581 tagaggacaa aaaggtacac ataaaaaagt ctttgataat atcaaaaaag aaggatttgt
     8641 aagagttaga gttaatggtg aaaactatga agttagtgat gatataatct taaataaaaa
     8701 tcaaaaacat aatatagaag ttgtagtgga cagaatagta attaaagatg gaatagaatc
     8761 aagactagca gattcaattg aaactgcagt taagctatct gatggtcttg taatagccca
     8821 aataatagat ggagaagaaa ttcttttttc aactaaattt gcatgcccag aacatggaat
     8881 tggcatagaa gaattatctc caagaatgtt ttcatttaat gctccctttg gtgcttgtga
     8941 tgtttgtaat ggtcttgggg aaagtaaaga agttgaccca gaattagtag taccaaacaa
     9001 agatttatca ataaaacaag gtgcagtggc agcatggggt tcaacaggtg ttaatgatga
     9061 cacttattac agtaaaatga taaaaagttt agcagagcat tttaatgtat ctttgacaac
     9121 accgtttaaa gatttaccag atgattttgt ggaggaatta ctttatggta aagataatat
     9181 aattgtagag tttacttatg agtcaaaatt tggaggaact agaaattata aatcatactt
     9241 tgaaggtgta atagtaaact tagagagaag atatagagaa actaattcag attacatgag
     9301 agataagatt gaagaataca tggcagagag accatgtcca aagtgtaaag ggatgagact
     9361 taaaaaggaa gttttatctg ttttagttga tggaaaaaat ataatggaag ttacaaactt
     9421 atctgtaaat gaattgattg actttatgga aaatataaat cttacagaaa aacagagatt
     9481 tatagctcat gagattataa aagagataaa aggaagagct atgttcttaa gagatgtagg
     9541 tcttgattat ttaaatttat caagaaaagc tggaacttta tcaggaggag aagcgcaacg
     9601 tataagatta gctactcaaa taggttctgc attggttgga gttttatatg tacttgatga
     9661 gccaagtata gggcttcacc aaagagataa tgataggctt ataactactc ttagacatct
     9721 aacagattta ggaaatacct taatcgttgt agagcatgat gaagacacaa tgagagaagc
     9781 tgattatgtt atagacattg gtccaggtgc aggtatacat ggtggtgaaa tagttgcaca
     9841 gggtacttta gatgaaataa ttgaaaatga gaactcaata acgggtcaat atttaagtgg
     9901 taaaaaacaa atattgcttc ctgagactac tagagaagga aatggaagtt ttatagaaat
     9961 agttaaagca tcagaaaata atctgaaaaa tattgatgta aagttcccat taggtaagtt
    10021 tacatgcata acaggtgttt caggttctgg aaaaagtaca ttaataaacg atatacttta
    10081 caaaggtgtt gcaagtaaag tgaatagact taagcaaaga gctggtaagc ataaggagat
    10141 aaaaggaatt gaaagtatag ataaagttat aaatatagac caaagtccta ttggaagaac
    10201 acctcgttca aatcctgcta cttatacagg tgtatttgat tttataagag atatttttgc
    10261 atctactaat gaagctaaag caagaggtta taaaaaaggt agatttagtt ttaatgtaaa
    10321 aggtggtaga tgtgaagctt gtaagggtga tggaataata aaaatagaaa tgcatttctt
    10381 accagatgta tatgttcctt gtgaaatttg taaaggtgaa agatacaata gagaaacatt
    10441 gcaagtaaaa tacaaagata aaactatatc tgatatatta gatatgaatg tggaagaagc
    10501 tgttgagttt ttcgaaaata taccaaatat aaaaagaaaa ttagaaactt taatggatgt
    10561 aggactttca tatattaaat taggacagcc ttcaactcaa ttatcaggag gagaagcaca
    10621 acgtataaaa ttagctgctg aacttagtaa aagaccaaca ggaaagactt tgtatatatt
    10681 agatgaacct actactggac ttcatatggc agatgtagac aagctaatcc aagtattaca
    10741 aagactagca gatacaggaa atacaattat agtcatagaa cataatttag atgttataaa
    10801 gacttgtgat tatgtaatag acttaggacc agaaggcgga gataagggtg gaactatagt
    10861 tgcaactgga acaccaaaag aagtatctaa agttgatggt tcttatacag gtcaattctt
    10921 aaagaaatat ttttcttaag ttatataaat tgtgaaaaat tttagagcag atatacgata
    10981 aactgttttg ttataagtaa attagtcaaa tataagttgt aaatagtaat acaaagtaaa
    11041 gtccatatta ctcttcatgg ttaacattgt tttcaaatca attatagaag ggaatatggg
    11101 ctttaacttg tttaaagcac tggaaattgt ataacttttt aaaattcata ttttagggta
    11161 taattttagt ataaaattta atgttttgga gggcgagatg tttgatatac aagagcattt
    11221 aaaaaaatta ccttctgagc ccggagtata tttaatgaaa gacaaatacg accatataat
    11281 atatgtggga aaagccatat ctctaaaaaa tagggtaagg cagtattttc aatcttcaaa
    11341 aaaccataca tctaaagtaa aatctatggt taagaatata tataagtttg agtatattat
    11401 aacggattca gagttagagg cattaatact tgaatgtaat ttaataaaaa gatacagacc
    11461 aaagtataat gtagtgctta gagatgataa aacttatcca tatataaaag taactacgaa
    11521 tgaagattat ccaagaatct taaaagttag aagagttctt aaagataagg caaagtattt
    11581 tgggccctat accaatataa ctgctgtgaa tgataccttg gagcttataa gtagtacgta
    11641 tccaataaga agctgtaaaa tagatataga taaagcaata aagaataaga caagaccttg
    11701 tttaaatcta cacataaata aatgtttagg accttgtaca ggaaatgttt ctaaagaaga
    11761 gtatggaaaa atgatagaag agattataat gtgtttatct ggtaaagaag aaaaattgat
    11821 ggaactttta aaggaaaaaa tgaatgagag ttctatgaac tttagatttg aagaggctgc
    11881 tgtatatagg gataagataa agagccttga agagatgata cagaaacaaa aaatagatgc
    11941 tactgtaagt gacttaaacc aagatgtggt tgcaatggca agagctcata atgaagcttg
    12001 tgtccaggta ttttttataa gaaatggtaa gattgtaggt agggaacatt ttatattaga
    12061 aggggtaatg gatagtccaa gagcttctat attaagttca tttgtaaaac aattttataa
    12121 tgaacaagag tatataccta aagaattaat tatagaagat gagatagaag atagttctat
    12181 attagaagaa tggctgtcat ctaaaaaggg gcaaaaagta acaataagag ttcctcaaaa
    12241 gggagagaag aaaagcttag ttgaaatggt taggaaaaat gctgtagaat atcttgaaaa
    12301 attttctgat atgaataaaa gaaaatatga aaaaagtatt ggagcattgg aagaattaaa
    12361 gcaaatatta aatctagaaa agttgccaat aagaatagag gcttatgata tatcaaatat
    12421 acaaggtgta gattcgattg gttctatggt agtgtatacg aatgctaaaa aagataagaa
    12481 ggaatatagg agatataaaa taaagacagt tattggacct aatgattatg attctatggc
    12541 tgaaattgta gatagaagat taaaacatgg aaatttacct gacttgatat tgttggatgg
    12601 tggaaaagga caagttagtg cagtaaaaaa agtactagaa ctcaatgatg tagatatacc
    12661 tctttgggga atgtacaaag atgataagca tagaactaaa ggattgatat gcaaagaaaa
    12721 agaaatagaa ctagataaaa caactaattt atatagattt attgctagta tacaagaaga
    12781 agttcataat tatgcaataa catatcatag aagtctaaga aataaagctt tgacaaaatc
    12841 tatcttagat gatatacaag gaataggtga aaaaagaaag aaatcattat taaatcactt
    12901 taaagatgta gatgctataa aaaaagctac tatggaagaa cttttagaag tagatggaat
    12961 gaataaaagc attgcagata atgtttataa tttttttaga aaggaagaaa attaatggaa
    13021 actagcaata aagtatcaat aagagagata ataaaagagt tgaagttaaa agtagtatac
    13081 atgcctgatg atgtagatta ttatgtctac tctcaagata taaatagacc aggattacag
    13141 tttgcaggat attttgatta ttttgcatat gaaagaatac aaatagttgg aaagacagaa
    13201 tataattttt ttggattaat ggatagtcat attcgggaag aagtactaga tagatttttc
    13261 tcatatgaaa taccagcgtt aataatttct agagacttgg aaatcaagcc agatgtaatt
    13321 gaaaaggcta aaaaatataa aagaatactt ttgagcacaa atagaaatac cactagactt
    13381 ataaatagac tgtcaaacta tttggataat aagttagcac cacatacaac aattcacggt
    13441 gtgctagtag atatttatgg tataggtgta ctgataaaag gtgaaagtag tataggaaag
    13501 tcggaaacag cattagaact gattcaaagg ggtaatagat tagttgcaga tgatgctgta
    13561 gaaataagaa aattagatga aagtatgctt gtgggtcagt cacctgaact aataagacat
    13621 tttcttgaga taagaggaat aggaataata gatgttagaa gtttatatgg agtaggggca
    13681 atcaaaaatt ctaaaatgat agatttagta gttcatttag aagcttggga tgaaactaag
    13741 tattatgata gactaggact ggataaagaa tatgaagaaa tacttggtgt taatgtagag
    13801 aaattagttg tacctgttaa acctggaaga aatactgcta tgatattaga agtagcagca
    13861 atgaatttta gacaaagagg aatgggatac gatgcagctc aagaatttac taaaaaatta
    13921 tcaaaattaa tcgatactaa gtagtactga ataatagtgg actacacatc atttaattaa
    13981 atattgatta ggattatgtc ctacacctta taaattaaaa tcaagttgta tatataatgt
    14041 ttattaataa gatatattat ataaggataa tttaaagtac agtgttagat tataataata
    14101 ctagattaat tttatatttg agtttcatta tataacaaga aagttggaat tttttatggg
    14161 aatagaatat tttagtaaac ttttagaaag tagtactgaa aaagaattgc tcttaagtaa
    14221 aaagtcaaat aacataagta cagcaagatt aatatctttt ttgatagtga tagcagggct
    14281 tgctattgga ttttataata aaaatatagt aggtattttt gttggagttt tatcaataat
    14341 aatatttata gcactcttag ttattcatag taaagtaaaa gaagaagaaa catattttaa
    14401 aagtaagagc gaagtattaa ataagtatat taagagattt ggagatggtt ggaaggaatt
    14461 taaaatagat ggtaaagaat acttaaaaga tgaaaattcg caagcaaaag atttagactt
    14521 atttggaaga gcatcattgt atcaatatat ctgtgtagct aatacatctt atgggaaaaa
    14581 ttttttagcc aaatatttgt ggaatgaaaa tccagatgag aatataattt tagaaagaca
    14641 gagagctata aaagaacttt tatcaaaaga tgatttttct attcatattc aaacactcag
    14701 taatataatt ggtaaagagc aaaaaaacaa ttcagatagt agtatagaat cgtttataga
    14761 atatggagaa aataagaatg tgtatatacc aaaatggatg catattttta cttggggttt
    14821 accaactgca acaatcttat cttttatatt ttgtatgtta ggatttttac ctgttttacc
    14881 tgttttttta ctttttataa tccagttagg gtttagtggt tttggatatc caagacttat
    14941 gcaaactctt gctccattat tttcatttag tagaagtata caagtatatg aaaaaatgtt
    15001 tgaggtttta gaaaaagaga cgtttgagag tgcatatttg aaagaattac aagataaact
    15061 ttctaaggga agtggagttt caagaggtgt taaacaatta aattcaatag gaaatgctgt
    15121 aaacctaagg tataatcaga tactatatat aattgcctgt ggtgttttga tgtggaacta
    15181 tcattgtgca gaagctttgg agcgatggaa aggtatatat ggaaatcaaa ttagagattg
    15241 gtttgaatct attggagaat ttgaagctct aataagttta acagttgttt ctcatgtaaa
    15301 ggaaaataca tgttttccag taataaaata tgaagatact ccaagattga aagttgaaga
    15361 ggtataccac ccattaatag cagaaaaaag tgtcatagca aattcgatac aattaaattc
    15421 tcagacttgt attatcacag gttcaaatat gtctggaaag acaacatttt taagaagtat
    15481 cggtgtaaac ttagtgctgg catatgcagg tgctccagta tgcgctaaga gttttgatgc
    15541 gacctgtatg gctatattta cttctatgag aatacaagat gatgtaagtc aaggaatatc
    15601 aactttctat gctgagattc ttagaataaa atcaatgatt caatatagtt taaaagaact
    15661 gccaatgttg gttctagtag atgaaatttt taagggaact aactctgctg atagaattat
    15721 tggagcaagt gaggcagtaa agaaattatc aaaaccctgg gttataagta tggtgacaac
    15781 acatgacttt gagctatgtg acctttcagg tagcggagat gttgaagttg taaactatca
    15841 tttctcagag tactatgtag acgataaaat tcattttgac tataagataa aagatggaag
    15901 atgtaaaact acaaatgcaa aacaacttat gagaatggca ggtattcttt aattaataga
    15961 taggttaatt aataaataag tctatttaga aatataattt aatttgtgaa attaataagg
    16021 attgtcttaa attacgtaaa agtattttaa gacaatcctt ttttaagaat aaattttata
    16081 ctttgttatg actacttagc ttcttcacaa gcacattctt gtggacactc tccatagaca
    16141 caactcttat caaagtaatg agtatgaagg tatttatgag ctacatcact atttggttct
    16201 ccaaagaagt ctttataaat agcttgtaaa tcaggattct catgagattt acgaatagcc
    16261 ttatttctat ctatttcata taacgcattt gcacgtctat caactatttc agtattacca
    16321 tggtgataag gttgaccagc acctccaaca catccaccag gacatgccat aacttctatg
    16381 atatggtatt tacattctcc acttctgacc ttatccataa tctttctagc gtttttaagt
    16441 gtatttacaa tacatacatt aactgtagtt ccatcaacat ctatacttgc ttccctaatt
    16501 cctttaagac ctctaacatt agtgaagttt acattatcta aagtcttatt agttatcttc
    16561 tcatatgaag tacgtagggc agcttctaaa actccaccac ttgctccaaa tatagatgca
    16621 gcacctgtag attttcctag aggattatca aaatcttggt cttgaagatt aggtaaatcg
    16681 attgcagctt cttttatcat cttagctaac tctctagtag ttatagaaag gtcaacatct
    16741 agtattccag attcacttaa ttcttctctt gaagcttcat attttttagc tacacaaggc
    16801 attacagata caacataaag ttcttcagga ttgattccta atattttagg tgcatagtag
    16861 tttttagcta tagaaccaaa cataccttga ggagacttac agctagatgc taagtttaac
    16921 ttgtctggat aattgtgctc taagaagttt acccatgcag gacaacaact tgttaatata
    16981 ggaagatttt cacctttttg gattctttct ataaattcag tagcttcttc cattatagta
    17041 aaatctgcac caaagtttgt atcaaataca tgttcaaatc caagtgcttt taaagcagct
    17101 accattttcc ctgtagagat tgaccctggt tctaatccaa actcttcacc aatagcaact
    17161 cttacagcag gagcaacttg cactactata gtcttttctt ttttatttaa aacatcccaa
    17221 agtttaggta cattatctac ttctgttaat gctccagttg gacagacaga tatacattga
    17281 ccacagaatg tacactctgt ttcaaccata tcagagttaa agaatgtact aactaaagta
    17341 ttaaatccac gattaactcc agataatgct cctactgttt gtatgtcatt acatacagtc
    17401 tcacatcttc tacataatat acacttagaa tggtctttaa ctaaagattt agtagaagta
    17461 tctttacctg caaaagattt agaaccttga tatcttattt ttcttactcc taaatcagca
    17521 gctatagtct gtaattcaca atctccattt ttttcacaga caaaacaatc ttgtggatgg
    17581 tctgataaca ataattcaac aatagttcta cgagcattta atgctttagc actattagtc
    17641 tgaactttca taccttcctt tataactgtt ccacaagctg gaactaatcc tctctctgtc
    17701 tcaaccatac aaacacgaca agaagcacaa gtgtcaagct tatctatttc attcatatgt
    17761 aaatgacaaa ggttgtgtat ttttatattt attaatttcg cagcatctaa gatagaagtt
    17821 ccactaggtg ctgaaacgtg tttgccatta atagttaaat taactaaact cattgttatt
    17881 cctcctccaa ataagttatt tcttgattat agcgttaaat gtacaattat ctatacaagc
    17941 accacatttt agacacttag aagtatctat tgtatgttgt tccttaacag aacctgttat
    18001 acaaccagca ggacacactc tagaacattt tgtacaaccc ttacagtcat ctgttataaa
    18061 gtaagaaagt aagttctgac atttaccagc aggacatttc ttatctacaa catgagctat
    18121 atactcatct ttgaaatatt gaagagtact taaaacaggg tttggagcag ctttaccaag
    18181 accacataat gaagcagttt gtatagtttc agcaaggttt tctaggtcat ctaaatcttg
    18241 tagagtacca ttaccttctg taattttagt taatatttct aataatcttg tagtacctat
    18301 acgacaaggt gtacatttac cacaagattc ttcaacagta aactcaagga agaacttggc
    18361 tatatctacc atacagtcag attcatcaag aacaagcata ccaccagagc ccatcattga
    18421 accgatagaa cttaatgaac caaagtctat aggagtgtct aaatgttcag ttggtataca
    18481 tccaccagat ggtccacctg tttgaacagc cttaaatttc ttaccttcag ggattccccc
    18541 accaatttca aatacaatat ctcttaaagt agtacccatt ggaacttcaa caagtcctac
    18601 attttctacc tttccaccaa gagcaaagac tttagtacca ctagaatctt cagtacctaa
    18661 gttggcaaac caatctccac ccttaagtat aattgcaggt atgttagcaa aagtctcaac
    18721 gttatttaag caagtaggag atttccatag accactctta gaagaactat aagttttcat
    18781 tctaggttca ccacgtctac cttcaattga atgcattagc gctgtacctt caccacaaac
    18841 gaaagcacct gcaccatatt ttaattctaa tttaaagtta aatccagtgc ctaagatatt
    18901 ttcgccaagt aaaccgtatt tttcagcttg agctatggct acttttaatc tttctataga
    18961 ttttggatat tctgctctta tgtatatgta tcctgtatca gaacctatag cgtatccaca
    19021 tattgccata gcttctagta cactatgagg gtcaccttca agtacagatc tatccataaa
    19081 tgcacctggg tcaccttcat cagcattaca tacaacaaac tttttatctg ttggaccagc
    19141 tggatttttt gaagcagctt cccatttaga gccagtaggg aaacctgcac cacctctacc
    19201 acgaaggcca gaagttttta cctctgcaat aacttcttga ggagtaagag aagttaaaca
    19261 tttgcctaga gctaaataac catcattggc aatatattct tctatactat ctgggtctat
    19321 aagaccacaa ttttttaaag ctatacgtaa ctgttttttg tagaaagaca tttcttcttg
    19381 ttcttcaact tttttactta aagattcttc ctcatataat aattcctcga caactgtatt
    19441 tctaattaag tggctttgaa ctatcttttc agcatcagat ggctcaactt ttacgtagaa
    19501 aacgttatca ggataaactt ttacaatagg accttgagca cagaaaccaa aacaacctgt
    19561 caaacgaaca ctaactttat cttggattcc tgatttcttt atttcatttt ctaattcact
    19621 aactatttct agactgtttg aagatgtaca tccagtatca caacaaacaa gtaattctct
    19681 tcttaataca ttttccttag aataattttt tcttaagcta agattaggtt tcagctcatc
    19741 agctaaaact tttaattcat caaatgaatt tactttacgc attatttatt cacccctctt
    19801 aacaattcaa ttctaattct ctataagtat ctaatatttc gcttacttgg tcaggtttca
    19861 ctttaccata aactttacca tttacagtaa caactggagc taaaccacat gcccccaaac
    19921 atcttaaacc ttctaaagaa aatttaaaat cactagtagt ttctccaact tttatgccaa
    19981 gctgtttttc aaaagcactt aagattttgt cagcaccttt tacaaaacat acagttccta
    20041 gacagacact gattttatac ttacctacag gttctgtagt aaaataagaa tagaatgtaa
    20101 caaccccata tacctttgca ggagctactc caagttttct agcaacatga agttgaactt
    20161 cttttggaag atatccaaat ataccttggg cttcatgtag aacttgtatt aacgcacctt
    20221 cctttgttgc aagagagtca ataaatatat ctagttcgtc aaataattgc ttgttgtgtg
    20281 aaataaaatc gcacataaca aatccccctt ttctttttaa agttattatt tttcataaaa
    20341 taattaacca tgagttgacc ccaacattat gtgggtgaac aaaaccaaaa caataaagaa
    20401 agaataatat acaacaattt ctttatttat aaaaacaatg caaaaaagtt acctattaaa
    20461 aataaaaact atactagtct attataatat aaaataattc agaatagtta aaaaaaataa
    20521 aaaaaatgag ctaaattcaa taaaaaaaaa ataatacata taaaaaactt taaaaaataa
    20581 accaaaatgt aaaattttta taaaataaaa gatttataaa actgctgata atagtcttga
    20641 aattgattcc ttgattttta tataagtaga cctagaatta tatatatcta atgttatttc
    20701 cttagaattt aaaatatcat tttcaaatat aattctttgt tccaatgctt tttttgaaga
    20761 gtacataaat gcattaactt cgaaattaag ctcaaaactt ctgatatcca tattagctgt
    20821 tcctatagag catatagagt catctataac tattgtctta gcatgtaaaa atgcattttg
    20881 accataagta tagattttag ctccaaactt taaaagttct ccagcatatg aatatgatgc
    20941 ccagtataca aagggatggt caggtttaga aggaatcatc actctcacat caacaccaga
    21001 taaacaggct atttttaaag tatctataaa agttgagtca agtattaaat aaggactctg
    21061 tatgtatata tactttctag ctttctgaat cattttaagg taaccatatt taatctcatc
    21121 aagttctgat atatctggac cacttgatac tatctgaatc ccaatatttt cagaaggatg
    21181 attagaattt gtatcttctt ctataaagta tttttctaaa tctaagtctt ctttagttgt
    21241 atatctccaa tcaaggatga atctcatatt taggtcaatt acgcaatcac cagtaagctt
    21301 agtatgtgta tctctccata gaccaaattt tggatttcta cctaaatatt catcaccaac
    21361 attatatcca cctacaaatc ctaccttacc atcaatgaca acaatttttc ggtgatttct
    21421 atagtttaag ttaaagttta caattttcaa gaatgatggg aagaagcttc caactttaac
    21481 accactttct ttaagtctgg aaagtgtact attttttagt gttctaccac caactgcatc
    21541 aaataaaagt cttacttcaa caccttcttt agctttgtct ataagaatgt ctattatttc
    21601 agttccgatt ttgtcatctt tgaaaatgta aaattgtatg tttatatatt ttttagcttt
    21661 tttaagttct tcaagaagac tgttaaaaaa ttgactagat tcagcataaa tccacacatc
    21721 gttattattg gtataatgag cattatttga attagccaat gcataaatca tatccttatg
    21781 ttgatgtata tctgaatcaa tttcagatgt agattggagt ttaacctgag tatctagtat
    21841 attacttttg attatcttat catccttttc tttcaatcta aacatattgt tttttgaaat
    21901 atttctgcca aaagctatat aaagtatgaa tccaagagca ggaatcaata ctaatataag
    21961 aagccatgtt aaagttgtat ttatgtttct tttttctcta aagataaggt ttagtataac
    22021 catgaaatta attatatata ttgtagtaac tgctatttca tatattgaca tttcgaaaaa
    22081 tgatatatca aacataaaat tccctccttc aaatatttat taaaataagt aattatatat
    22141 aataacacaa acatatatct attatatata atacctcaat aattaaaaag taaaataaat
    22201 aatcaaaagg taaatttttc aaattcaata taaattattt cgaaattaac aaagttgtta
    22261 tataataaat agtataatta tatgagacaa gtatatataa gtatatagta tattttttat
    22321 gttaataaaa tttattatat atcatattgt tattatataa ttattgccat aaaaagacaa
    22381 gggaaagtat aatttttaag ggggaaggat ttatgagtaa aggattcttg acacctgggg
    22441 aaactgcaca agcaactata agtgcaggca ttaaaaaagc aaatatttcg acacaaaatg
    22501 caattctatt aggtttattt ggaggagcat ttataggttt tggggcattg gggagtatga
    22561 tagttagtca aacttttggc aagatagacc caggggtagc tagcttttta agtgctatgg
    22621 tctttccagt aggtcttatg cttgtagtag tggcaggagc agaattattc actggaaaca
    22681 ctttaatggc tattgcactt atggacaaaa aaataacatt aggaaaaatg ttaagaaact
    22741 ggggactggt ttattttgca aatttagtag gttcaatttt gctagttgca cttgtgtttt
    22801 attcttctac attgacagga gatgcagcta caaaagctat agctgtggca gaagctaagg
    22861 ttaattcaac tatagcatca atgtttttaa aagcaatact ttgtaatatt ctagtcgttt
    22921 tagctgtatg gatggctaca gcatctcaag atataatgtc aaaattggct tcatgttgga
    22981 cagtaataat gctatttgta ttatgtggat tccaacatag tgttgcaaac atgtttttta
    23041 taccaatggg aatggcatta ggagcaaata taactatggt tcaatttata actaatttgg
    23101 tatttgtaac acttggaaat atcgttggag gttccatagt agttggaggg atttattatc
    23161 tttgctatgt caaaaatagt tagataaata atacgaatta aattgccttt taaagcattg
    23221 atatcagtga tattgatgct ttttttaaat tatttatatt ctttta
//
LOCUS       DAYATN010000051        23076 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.051, whole genome shotgun sequence.
ACCESSION   DAYATN010000051 DAYATN010000000
VERSION     DAYATN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.051"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(82..459)
                     /locus_tag="ACGI8A_001891"
     CDS             complement(82..459)
                     /locus_tag="ACGI8A_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0179888.1"
                     /translation="MIILKKIPKSELLVMNFIWDKDIDKIPAREVANYMEISHGWTKG
                     TTGKVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISS
                     LGCGNEISDDDIKELEQWLKKRK"
     gene            complement(978..1400)
                     /locus_tag="ACGI8A_001892"
     CDS             complement(978..1400)
                     /locus_tag="ACGI8A_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFH0179889.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1878..4781
                     /locus_tag="ACGI8A_001893"
     CDS             1878..4781
                     /locus_tag="ACGI8A_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0179890.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            4814..5260
                     /locus_tag="ACGI8A_001894"
     CDS             4814..5260
                     /locus_tag="ACGI8A_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0179891.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKKPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            5383..5685
                     /locus_tag="ACGI8A_001895"
     CDS             5383..5685
                     /locus_tag="ACGI8A_001895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0179892.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            5866..7272
                     /locus_tag="ACGI8A_001896"
     CDS             5866..7272
                     /locus_tag="ACGI8A_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFH0179893.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            7616..8674
                     /locus_tag="ACGI8A_001897"
     CDS             7616..8674
                     /locus_tag="ACGI8A_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFH0179894.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVNIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            8773..9459
                     /gene="araD"
                     /locus_tag="ACGI8A_001898"
     CDS             8773..9459
                     /gene="araD"
                     /locus_tag="ACGI8A_001898"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFH0179895.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            9477..10124
                     /locus_tag="ACGI8A_001899"
     CDS             9477..10124
                     /locus_tag="ACGI8A_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFH0179896.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(10297..11664)
                     /locus_tag="ACGI8A_001900"
     CDS             complement(10297..11664)
                     /locus_tag="ACGI8A_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFH0179897.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(11854..11942)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            12370..14490
                     /locus_tag="ACGI8A_001901"
     CDS             12370..14490
                     /locus_tag="ACGI8A_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFH0179898.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRLFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            14594..15847
                     /locus_tag="ACGI8A_001902"
     CDS             14594..15847
                     /locus_tag="ACGI8A_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HFH0179899.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVIAMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(16001..17062)
                     /gene="ytvI"
                     /locus_tag="ACGI8A_001903"
     CDS             complement(16001..17062)
                     /gene="ytvI"
                     /locus_tag="ACGI8A_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HFH0179900.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            17336..18076
                     /locus_tag="ACGI8A_001904"
     CDS             17336..18076
                     /locus_tag="ACGI8A_001904"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HFH0179901.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            18088..19866
                     /locus_tag="ACGI8A_001905"
     CDS             18088..19866
                     /locus_tag="ACGI8A_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0179902.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            20033..20950
                     /gene="pfkB"
                     /locus_tag="ACGI8A_001906"
     CDS             20033..20950
                     /gene="pfkB"
                     /locus_tag="ACGI8A_001906"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFH0179903.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     IFKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            20987..22906
                     /locus_tag="ACGI8A_001907"
     CDS             20987..22906
                     /locus_tag="ACGI8A_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFH0179904.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         9184 a         2499 c         3923 g         7470 t
ORIGIN      
        1 taaaatgaaa aaaagaatta gtggacttat gtaaatatta aatttaataa cttctataaa
       61 taattccata aaatacgtct cctattttct tttttttagc cattgttcaa gttcttttat
      121 gtcatcatct gatatttcat ttccacaacc taatgatgat ataaagctat gaatagaatt
      181 tttatgtata agctctaaaa aatcctttgt ttcaaatttt agatattcct catgtttaac
      241 tttaatagtg tataaagtat ttctaccatc tttttcaaat tctaaaaaac ctttatcact
      301 taatctagaa agtactttac cagtagtacc ttttgtccat ccatgagaaa tttccatata
      361 attagctact tctctggctg gtattttatc tatgtcttta tcccatatga aattcataac
      421 taacaactcg gattttggta tttttttcaa tataatcact cacctttaac ttgattatat
      481 tagacaaatg tctaatagtc aatataatat atcatcatgt ataaataatc tttatattat
      541 tttatgcttg agaactaagt agtatagatt tatagataaa aaagatattg agagttttat
      601 ggaagagggg aatatatttt ctattagaga taagaatatg acatatattt tttattgatt
      661 tttacaaaaa tactatatag tgaataaatt aatatctttt ctaaattaga agaggagaat
      721 cttattagat aaagattatc ctctttattg aaagtttaag ggatattaga gattattaat
      781 ttttctctct ttttttaact aaaagcaata atacataaaa ttattattgc taatgatact
      841 cttaatgtgg tatatagtca tagtgtcaca tgtttatcaa tagttttttc attaattatt
      901 gtaacgaata gtaggactca taaaatactt agaaatatgt ttttagatag attttaaata
      961 tataatctaa cttatatcta taaattaaca ctattattct ccaattccat ttctagtttt
     1021 tttataaatt tattaaattc ttctggttgc ctttttccaa acaatactct tacaagagct
     1081 gaattgattt ttccaaatat attcctcttg ctctcacttt caataaaaat aagttttgta
     1141 ttttctaagt caatatgtac taattcatat ctagtaatag tactttctct tttattgctt
     1201 gtttttatct catatactct atttttttcg aaattagtta tttcaacctt aaatctttta
     1261 ttattttttt gctgtacaaa tgttcctact gcttttttct cattaaaatt aggaaaattt
     1321 agtcttgcag attttgtaaa tatattaaat atgtcttttg cagaatattt taataaaact
     1381 tcagttttaa atgtattcat atcgttttcc cttctgtata taaatttaat atttataagt
     1441 taatttttaa gttaaaatag tataatatgc catattaaat catgttaaaa ctatttatat
     1501 agctaaaatc ttacctatat aagtatgtat actatcatat aaaaataaaa tcttaaaata
     1561 gaaagggtta aaaacacagt ttattataat ttttattgtt gagtagatac gtactattat
     1621 atatgattga gcaagttttt caatcataaa tttataaagt attttgcatt aaaatttaaa
     1681 caaagaatta aattatttaa gattatgaat aactaataca tttagtaaat tactaaatat
     1741 acaaaatgta aatacttatc aaaatgtata aatacttatg acatctagtt acttacaggg
     1801 tttaagtaga aaaaaatatc aaagtaagta tggcatgata gttgcttata tatttatcaa
     1861 gaaaggagtt attaagcatg gagttgagag ataaacttgt aaacatcata aatacagaaa
     1921 ataaaaaaga tccattaaca gatgttcaaa tagcaaagtt tttaagtaca actagagaga
     1981 acataactaa tttgagaaaa gaacttaata taagtaattc aaggcaaaga agatatcctt
     2041 atttaaaatc agcaatttcg gcaatattac aaaagaataa gaatatttct atatcagaaa
     2101 ttacaaggga attgatgaca gaaggcttta atatatcgag aagagttgtg gaggaacttt
     2161 tacctaaaga aagtttagaa atggatgtag tagaggaaga tgaagaagaa tcgaatgacc
     2221 catttgaaac tctgataggt aataaaggga gtcttagaaa tgctgtagag caggctaaat
     2281 ctgctatatt gtatccacca aaagggttac caacgttgat tattggggaa agtggagttg
     2341 gtaagagttt attttctaga catatgtatg agtttgcaaa gcaaaaaaag atagtaaaag
     2401 agagctccaa ttttgttgta tttaactgtg ctgattatag tgacaatcca caattattat
     2461 tatctttgtt atttggatat aaaaaaggtg catttacagg tgctgaatat gatacaccag
     2521 gtcttgtaga agaagctgat gaaggagttt tatttttgga tgaaatacat agattgcctc
     2581 caaaaggtca agaaatacta ttttctatct tagatagggg aaagtttagg agattaggtg
     2641 aaactaatgc tgaaagaaaa gtaagtatta tgttgattgg ggcaactact gaaaatgtag
     2701 agactaattt attattaaca ttcagaagaa ggataccaat gctaataact ttgccaccat
     2761 tacatgatag attactaaaa gagaaaatag accttatata caatatcttt caacaagagt
     2821 gtaatagaat aaatgcaaaa atatttgtag ataaaaatgt gattgaaata cttgcactaa
     2881 aaaagtttag tggaaatata ggacaattac aaaatatgat tcaggttctt tgtgcaagag
     2941 cttttatgaa gtttataaat tccaaagatg aagatagtga atctatagta gtagtagata
     3001 taaatgaagt tttaaagctt aaagattcgt ttaaagatgc tgcctttcaa gagattgaat
     3061 atacagagat aagaaaatat ttgaaaaatg ctatatttat accatttaat ttagaagaat
     3121 catcagtaaa aggaagtctt ttaagtgatg aatatatatt accagaggac atatacaaaa
     3181 caattgaaaa gaaatattat gacctaaaat ccctagacat tgaagatata gatatagaga
     3241 atattttatg gacttttatt ttaaatagat ttgctgactt aaagtttaat cttgatagta
     3301 atgaagaaat tttttcaatg agtgatttaa atagttttgt aagtgatagt ttagttaaac
     3361 tggtaaaaga acttatgaat gatatagttg aaaaaaatat gaatttagaa gtaaataaaa
     3421 atatgttcaa atatctagca atacacttag aagaagctat aaaaagaatt aagctaaatc
     3481 aaaagataat aaatgtaaat ttagaaaaaa taaagattga cttttcaaaa gaatatgaaa
     3541 ttagtaaaag ttttgcaata aaaattgagg aatcaattgg tattaagatt tcagatgatg
     3601 aaattggatt tataacgctc tacataaagt ctgctctaaa aaatgaagtg aaaaaagata
     3661 aagtagggtt aataataatt tctcatggac gtatagctac agagactgta aatgtagtta
     3721 aagaactttt aggtgtaaag tttcctgtgg ctatagatat gcctttagat gaaaaaccaa
     3781 taaatatata caataaggca gtagaattgt caaaaataat agaccaaggg aaaggcatat
     3841 tgtttttggt ggatattggt tcacttacaa acataggtca aatagtaaat aagagaacca
     3901 atataagtac taaaactatt gatagagtag acctcctaat ggcactagaa gcaactagaa
     3961 aagtttctat gggagaggaa gaacttgatg aaatattttt ttctttgcta aaagatagga
     4021 tgggatataa ctatgaatta gaaaaacaca tagataaatc aaatgcaatt ataacattat
     4081 gtcttactgg agaaggaact gctaagtata taagtaaaac gttggaagaa aagtatgata
     4141 acactaaatg ttatcaaatg agtgcattgg atgaaagttt attttgtaaa attgaaaatc
     4201 taaaagagac aaacaatata ttagctattg ttgggacaat aaaccctaag ataccaggta
     4261 taaattttat accctataat aaagaacttt tcaaaaattt ggatatctat ctatcaaaat
     4321 caaaacaaca agatgacaga ccaattaaat cgtatgaaag aatgcttgat gaggatttag
     4381 tgatttttga accagatata tattttaaaa aggatttatt agaatatgta tgttcaattc
     4441 ttataaataa agaatatgta gaaaaagatt acttagattc tgttttacat agagaagaaa
     4501 tgcttccaac atattctaaa ggagctatag gtgtgcctca tggagattct tctactgtaa
     4561 atagtacaag gtttgttttt gttaaactaa agaatcctat tgattggggt gtaggtaatg
     4621 taaatttcat atttatgcca gtttttcagg ctaatgataa agagattgtt aaaaatgttc
     4681 ttgagatact aaaagatgca gaatttatga acaatgccaa taagtgtttt gataaagatg
     4741 cttttagaag gattatattt gataaattta aacatctgta gccatagcaa aaaaattgag
     4801 aggagttaga gtaatgaatg tagatttaat atcattgggt ttagaatcta atgattatga
     4861 aagtgttatt gaagaattag gaagtataat gtgtaaaaaa gaatatgtaa aagaaacata
     4921 cataaatgct gtcttggaaa gagaaaggac attaccaaca ggtctagata ttggagacat
     4981 gtgtgttgct attccacata cagattcaaa gcatgtaaat gagtctaatg tagctgtagg
     5041 gatattgaaa aatcctgtaa agtttaattc aatgatagac ccaaaagata gacttgatgt
     5101 agaacttgta tttttattag cagtaaagaa gccagactca caagtaaaac tgttaaaaga
     5161 tttaatgtca gtttttcaaa atataaagtt attaaaaaat ataaaaaacg catcaacaaa
     5221 agaagaagta gctaaattat tggattttat agaaatttaa tgtaggagaa ttttataaat
     5281 aaaaaattaa aatttaaaca atcaaagtaa gtattatttt tttaataggt ttaatcttat
     5341 ataaaataaa aatttagagt gaaaaattag gaggaaatta ttatgaaaca atttaatgta
     5401 ttatcagttt gtggttcagg gacagttaca tcatctatgg tagcaggtaa attaaaagaa
     5461 gtactaggta ataaggggat ttctattact acaacagaag ctagaccaac agaagcacta
     5521 aatttagcac aatcaggaag atttgacttt ataacattta cgagtccact tcaaacaggc
     5581 gattatggaa taccaactat aaatgcattt gcttgcctaa ctggtatagg ggaagaggca
     5641 ttctttgaag aagttttgac tgttattgaa gggatagata agtaagtttt ggagacgtga
     5701 atttatagta tttaaatcaa tgatttagat actataataa gctaatatta actaaatatt
     5761 tgtggaaaaa acattttata tgttgtcata aattaaaaaa ttataaaaat agtcacttaa
     5821 gtattaaatt aagcttataa atatttataa gtaggaggga aaaagatgtt agaatttatg
     5881 aaatctataa tcgatacatt tggttctgca attatagttc caattataat attcataatc
     5941 gcaaagatat ttaaagtaac tactaagaaa tcgtttcttt ctgctgtgta tgcaggagtt
     6001 gctttacaag gttttacact gattttaaat tcatttacac cagtaataac accagttatc
     6061 aataggatgg ttgagagtac aggagtaaac ctaccagttt ttgatgttgg ttggcaagca
     6121 acatcattgg ttgcattttc aacaagtgca ggtatgatat atttaggtct tggtatatta
     6181 ttacaaacaa ttttattttt gataaaatgg acagatgtgt ttcaaccatc agatttatgg
     6241 aataactatt cttatatggt atggggagca atggttatag gagtaacagg aaattttcct
     6301 ttaggtatag catgtatggt tttattaaac ctatatagtt tattaatatc agagttagtt
     6361 gcaaagagat ggtctagtta ctatagatat cctaattgta caataatagc tatgcataat
     6421 gttgaggctt ctgtgtttgc agtatttgca gaccctatat acaataaatt gggattaaat
     6481 aaaataaaat taaatccaaa agaattagag aagaaattag ggtttttagg tgaaccaatt
     6541 actttaggtc tttttttagg tatgtttata ggtatattag gtaatatgac gagaataaat
     6601 actatggaag cttggggaga aataatgaaa gtaggtatat ctacaagtgc agtgatggca
     6661 attttcccta aagtagcaag tatgtttgct caagcatttg cacctataac agaagcagct
     6721 agaaagatta tgcaaaaagc tggaaaccgt gagtggtaca tagcagtaaa tgatgctgtt
     6781 ggatatggtg aacctgcaac tttaatatca ggtcttatat taattccaat aatgcttgta
     6841 atagctatgg tacttcctgg taataaggta ttaccagttg tggatttatt ggcaattcca
     6901 tatatggttc aaggtttagt tgccatacat aatggtaata taccaaaagt attagtttca
     6961 ggtataattt ggtttggatt aggattatat gtttgtacat ctacagcacc attatttaca
     7021 gatatggcta caaatatagg tgtagctata cctgcaggag caatgttaat aacaagtttt
     7081 aatatactag gaaaaccttt gatgggatta gttttctttg catttctaag tgcaaatcca
     7141 atttatatag gtctttcagt tgtaatttac tttgttctat gggcattgtt tagaaagaac
     7201 aaaacaagta ttcttgatta tttagaaaaa caagcactta aaaatgtaga agaagagcct
     7261 gtagcagttt aagtttataa aaatgaaata aaaataatta aaggagttta taattgattt
     7321 tatataagta tcagataata cctgagaaat atttaaatga aatatagact tctttttatt
     7381 tgataatatt tgtaattata atgcattgta tttttatagt tgagttttct gtataacctg
     7441 ctatttaaaa catactcttt tatatttata cagtaatgtg gtttaatttt ataaaatgta
     7501 atctctactg gtaaaagact ttgtatataa ggcatttgcc tgaataaacc atttatcttt
     7561 tgtatggcat agatattgct atatagaagt atatagcatg aaaggagata tttatatggg
     7621 aaataaaatg agagcatcag tattgtataa tgtaggtgat gtcagatatg aaatggtaaa
     7681 tataccagaa ataacagata cgcaagtgct tgtaaatgta aaatatgttg ggatatgtgg
     7741 ttcagattta cctaggtcta tggtaagtgg tttgagtgga aatacaaaat atccattgat
     7801 tttagggcat gaattttctg gtgaagttgt taaaattgga gaaaaagtaa agcatattaa
     7861 tgtaggagat agagtagctg ttgcaccatt agtaccttgt ggaaaatgtg attattgtaa
     7921 tgaaggtaat tttggcttgt gtgatgatta caatataatt ggaacaagag taaatggagc
     7981 gtttgcagaa tatgtaagag ttccagaaga acatatttta aaactaccag atacattgga
     8041 ttatgaaact gctgccggaa ttgaaccagc aactatagct tatcatggga tttcaaagtc
     8101 taatattaga gtaggagata gtgtagttgt gttaggatgt ggtccaatag gtcagtttgt
     8161 tatacaatgg gcaaaggtgt ttggtgcatc taagattata gcagttgata tttttgatga
     8221 aaaattagaa ctttctaaac ttctaggagc aaactatata cttaactcaa aagaagtaaa
     8281 tgtaatcaaa gaaataaaaa aaataacaaa tggtggtgct gatgttgtaa tagaaactgc
     8341 tggtagccga tttacacaag aacaatctct atttgtagca aaaaagagag gaaatatagt
     8401 gtttgtaggt atatcacaca cagaattacc acttagtgca gacgctactg agtgtatatt
     8461 acgtggagaa cttaccttga aaggttcttg gaattcatat acaagccctt atcctggaag
     8521 agcatggact gcaacattgg attttatgga aaaaggtgac attattttta aacccatgat
     8581 ttcagataaa ataggtctta atgaagtggg tgacttttta agtaaaatga gcaagagaga
     8641 aattaatttc aataaaatcc tagtagaaat ataaaaattt aataaaaaat tctaaaagca
     8701 attaaatttt ataactatga cattaaataa aataaaaata attttcttaa ataaaatata
     8761 ggaggaagaa aagtgttaga aaatttaaaa gaagaagtat taaaagcaaa cttagaatta
     8821 cctaagaaaa agctaattac ttatacttgg ggaaatgtaa gtgggataga tagagaaaaa
     8881 ggattagtgg tgataaagcc aagtggtgta gagtatagta atatgactgt agaagatatg
     8941 gttgtagttg atttagaggg aaatatagtt gatggcaaat taagaccttc ttcagatacc
     9001 ccgactcatc tagttttata taaagagttt gaagaattgg ggggaatagt acatacacat
     9061 tcaagttggg caacaatttg ggctcaaata ggaaaatcta taccatcttg tggtactact
     9121 catgcagatt atttttatgg aagtatacca tgtaccagaa aaatgactag agaagaaatt
     9181 tgcaatgaat acgaaaagga aactggaaat gtaatcattg aagaatttat gggaagaaat
     9241 ccaattcatt gtccaggaat aattgtaaat gaccacggac cttttacatg gggaaaagat
     9301 gcaaatgagg ctgtacacaa tgcagtagtt ttagaagaag tcgcaaagat ggcttattat
     9361 acagagttaa tgagtcctga taatattatg gataaggttc ttatgaataa gcatttttca
     9421 agaaaacatg gaaaaaatgc atactatggt caaaaatgat tatctggagg tgaattatgc
     9481 tagactcttt aaaaggtaaa gttgtggaaa tagcaattaa agcagagcat tatggtttat
     9541 gtaaagaaaa gactggtaat tttagtatta gagataagga tactggatat atacttataa
     9601 ccccatcagg tgtaggaaga gaagaattaa aagttgaaca tatttgtgta ttggatttgg
     9661 atggaaatgt aatagaagtt gagaaaggta ttaagccaag tagtgaggta ttgatgcata
     9721 ttgaaatata caaaacaaga gaaaatataa atgccatatc acatactcat tctttatatg
     9781 caacagcatt ttcagtagca aagaaaccaa ttataccaat tgtatatgaa tcagttaatt
     9841 atggcggtta tgtagatatt gcttcatacg aaagaccagg aactttaaaa ttagctaaga
     9901 gtgtttcaaa attactgata gatacagatg catgcttatt agaaagacat gggttgacaa
     9961 ctgtaggtaa tgatttagac gaagcattat taaaatcaag atatgtggaa gaggtagcag
    10021 aaatatacta taggtcttta gtattaaatc aatttaaaga acttgaagta gtagatgttg
    10081 atgagtttaa atcttggaaa tacccagaag aaataaagtt ttaatctaaa taattttcat
    10141 tgaagtggtt aattaaaata tatgtattat ttatatcaat tactatgttt tattatgtta
    10201 attaccgtat tttgcaaata tatttattac attttttcaa aaaaatatag aatccgaaag
    10261 ccatataaaa ttgacttggg attctatact ttttatttat gcatttttct ttatctgatt
    10321 ttctggagaa tttatatgtt caaaatattt tttagtttca gatactatta taggtgataa
    10381 tgcaagtaaa gctataaggt taggtattgc cataagccca tttacaatat cagcaattat
    10441 ccaaacaact tctaacttta agaaagcacc taataacacc atagccacaa atacagttct
    10501 atacccattc atgcctttta caccaaacag aaattcaaaa catctttctc cataataact
    10561 ccatcccaaa attgtagtaa atgcaaataa tgttaaacta actgtaagta atattggtcc
    10621 aaatgtagga attgctccgt taaaagctgc ttgtgtcata acagctccat ttaggtctga
    10681 acaccacact cctgaaataa caagagagaa tccagtaaga gtacagatta taatagtatc
    10741 tataaaagta cctgtcatag atataagacc ttgttccgct ggccattcag ttttagcagc
    10801 agctgcagct attggagcac tacctagacc agactcattt gaaaatacac cacgagcgat
    10861 accatttcta attgccagtg acatagttgc tcctaaaaaa ccaccagtag cagcagtagg
    10921 tgtaaatgca cttttaatta ttaacataaa agtttcaggg attttattcc aaaaagatat
    10981 taaaatgatt ccacagatta caacatatat aactgccata aaaggtacta ttttagtagc
    11041 tacattagat atacttttaa gtcctccaaa tacaactaaa gctacaaata ctgctaatac
    11101 aacccctgta attctagggt ctatcccgaa actattattt attgcatctg ttattgaatt
    11161 tacttgagta aaagtaccga ttcctaaaag tgcaactagt ataccactta tagcgaaaaa
    11221 tattgcaaga ggtctccatt tttctcccat accatttact atgtaataca ttggcccacc
    11281 agacacttga ccatttttat ctttagttct gtattttatt gcaagaacac cttctgaata
    11341 cttagtagcc attccaaaga atgctgctat ccacatccaa aaaagtgcac caggtccacc
    11401 agctttgata gctgtagcaa caccaacaat attaccagta ccaactgtag cagctagagc
    11461 agtacataag gctgcaaaac ttgaaacatc accactacct ttgttttcag cagagaatat
    11521 taacttaaga gctcttggca gtttagttat ttgaacaact ccaagcttaa cagtaagtag
    11581 tataccagta ccaactaata gtaccagtag gggtggtccc caaataaaag catcaacttt
    11641 gtttaaaata tcaattagat tcataaaaac actccctcat tttttttgct ttacataatc
    11701 aatagaattt ttaatgaagt aattgacagg aaataagtat aatttattaa taatttctag
    11761 tgtaataaaa aaagctaaat ctaaaaaaga ttcagctctg gatgtacact aattagcaac
    11821 caaaataaaa gtattagtaa atactgtttt tggtagataa tccctgtcct tttgcctgag
    11881 agattgagca taaaagcctt gccccttcgg cgcccataat gggtctctcc agagttccgt
    11941 aaattttaca gtcctacttt taaaagtacc tgagagtgtt actccttcgg tgtgctatat
    12001 agcaactctt ctataaaatc ttaaccggat attgatttgt catagcttat tgtattaata
    12061 atattaatat aagtttttat gttttgtcaa ctaaaaaatt aaaaaacaaa gaaaaaatat
    12121 aaaaatgtaa gcctatttac gaattataaa cttagaaaga tgagtaatat acgtgaaaat
    12181 gaattttgta atacacatac aaatgaagtg taaaataagt tttaaataaa aaaataataa
    12241 aatcttaaat aattaattag atatgattaa attatggtaa tattatagtt acgatattaa
    12301 gaaaagttct atttagttag attttaagcg taatatgaga ttatgtatag agaaaggatg
    12361 tatttttaga tgggaaaaat attagtgcta gcagaaaaac cttctgttgg aagagatata
    12421 gcaaaagttt tagggtcaaa aaatgaaaaa aatggttata tagaggggcc aaagtatgtg
    12481 ataacttggg cactaggtca tttggttaca cttgctgacc ccgaaagtta tggagaaaga
    12541 tataaatcat ggagcttaga agacctgcca atacttccca agcaccttaa gactgttgta
    12601 ataaaaaaga gtggaaagca gtttaataca gttaaatcgc aaatgaatag aaatgatata
    12661 gatgaaatag ttatagcaac agatgcaggt cgtgaaggag aacttgtagc aaggtggata
    12721 atagaaaaat cacaggtaaa aaaacctata aaacgtcttt ggatttcttc aagtacagac
    12781 aaagcaataa aagacggatt tgcaaaatta agaagtggaa aagaatatga aaatttgtat
    12841 tactcagcaa tagcacgagc agaagctgat tggattattg gaataaatgc aaccagagct
    12901 ttgacaacaa agtacaatgc acagctttct tgtggaagag tacaaactcc tactcttgca
    12961 atgttgctta aaagagaaga agaaataaga aatttcaaac ctaaagagta ttatggatta
    13021 gaattaattg ctacaaaggg aaattcagat ataaaattta tttggaatga taaaaacaat
    13081 aattcttcaa cttttagtaa agaaaagata gaatccacat taaaaaaagt aaaaggtgta
    13141 gacataaaaa tatctgaagt aaacaagtca tataagaaaa aatattcacc tgcactctat
    13201 gatttaacag aattacaaag agatgcaaat aaaatgtttg gattttcagc aaaagaaact
    13261 ttatctataa tgcaaaaact gtatgaacat cataaagtac ttacttatcc tagaactgat
    13321 tctagatatt taacatcaga tattgttgga acattaaaag atagaataaa agctgtaaat
    13381 attagtgatt attcaaagat ttgtaataga ttattaaaaa gcaatattat agctaataaa
    13441 tcgtttgttg acaattcaaa ggtaacagac caccatgcaa taataccaac agaacagaag
    13501 gtattcatga gtgattttag tgataaagag cgaaaaatat ataatcttgt agtaaaaaga
    13561 tttttagcag tactatctcc tccttttgaa tatgagcaga caactataag tgcaactgta
    13621 gaaggagaaa tatttaaggc taagggaaac aaagtaataa atttaggttg gaaagaaagt
    13681 tataaagata ttgaagatga agatgagtat gaaagtatac caaatgtttt agaaaaagat
    13741 gtacttaagg tcaagggatt aaagataact tcaggaaaaa caaatccacc accatactta
    13801 aatgagggaa cattattgac tgctatggaa aagaatggtc tagggacagt tgctacaaga
    13861 gcagatataa tagaaaaatt attcaattca tttttaatgg aaaagaaaaa taaagatata
    13921 tatataacat caaaaggaag acaattatta gatttagttc cagtagactt aaagacacca
    13981 gaacttacag cttcatggga aaagaaatta ttggatattt caaagggaaa acttagtaaa
    14041 aatacattca taaatgacat gaaagattat tcaaaagata ttgtactaga agtaaaaaat
    14101 agtgataata aatttaaaca tgataactta actaaaaata gatgtccaga atgtggtaaa
    14161 tttatgttag aggtaaatgg taaaaaaggt aagatgttga tttgtgaaga tagagagtgt
    14221 aatactagaa aaactatatc acaagtaacc aattcaagat gtcctaactg ccataaaaaa
    14281 ttagaacttc gtggagaagg tgaggggaga ctcttcactt gtagttgcgg atacagagaa
    14341 aagttgtctt ctttcaacaa gagaaagcaa gaagaaaaac agaaggcatc aaaaaaagat
    14401 gttaatcaat atttaaaaaa tcaaaataaa aatcaagaga catttaataa tccatttgca
    14461 gaagcattag ctaagctaaa aaacaaataa ttttaaagtt attattatac acaattataa
    14521 ctttaactgt atattagtta tatgtactga tataatatag tgtatagttt aggcatgaaa
    14581 gtagggctgt taaatggatt taagtggcat atttaaatac tattgtaaag agtgtgaaaa
    14641 tacatggaat aattcgagtg ttgaattatt tgagaatata gaaacgtata gtaaagattc
    14701 acaaaaaaag agggaaaaag aattagataa attgctaaat acaatatcag ttcatttaga
    14761 gaggtatcca agtgatgctg tattgagaaa aatgtgggta aaaaagggcg aggttttctt
    14821 acaaaagaca ttggaaaaag aaaatatttt taagttagaa aaaatggatg tagaggatag
    14881 aaaaaaattt ttagatataa caaaacagtt tattagagat gctagaaaat ttgatgatga
    14941 tttacctata ggtgatatta tgcaagctat gaggaatgta tggatttcaa atgcattaca
    15001 attattattt ggtaaagaag tatattattc aaaagctaac tttgcatata gtatgttata
    15061 tccatatact gataattatt tagacaatac aaatatagat aaaaatgata agattttatt
    15121 taataactgg ttagaaaaaa ggctcctggg agaacacatt aaatctaagg attatcatga
    15181 aagtaaagta tctcaaatga tagattatat tgaaagtgta taccctagag aaaagtttac
    15241 agaagtttat gaatcgttat tattaatatt taaaagtcaa gtaaatagtt taaaacaaca
    15301 tggtaaggaa aatcatttgt gtaaagaaga tttattatcc atttctattg aaaaaggagg
    15361 ttcatccgtt ttagtagatg gatatttaat aagtggattg atgacaaagg aagaaataga
    15421 gttttgtata ggatatggat ttttattaca aatatctgat gatttacaag atataaaaga
    15481 agatttaaaa tacaaccata aaactattat tacagagatg tcaaaagagg gtactttaga
    15541 taaagttgta aataaactaa taaattttac tattgagtta atagatagtt ttaaaattaa
    15601 taataaaaat aaatctgtaa tagctatgat aaagaatgat tgcttaatgt taattttatt
    15661 ttctgtagtt tataatgctg aatttttttc tgtaggatat ataaaagaag tagagaaatt
    15721 tattccatat acaatagatt attcattaga gattgaagaa aaaataaaag aaaaatttaa
    15781 aaatatagat gttttaaata atgaaaatga atataaagaa atgattgata ttatttgtgc
    15841 agagtagaaa aaaagggagt gtcttagaat agacttttta gtttatgagg cactcttttt
    15901 gtataaaatc aaatgtattt tcatcatttt aacaattaaa aagagcttat ccctatttga
    15961 gaaagccctt tttaatttaa ataaaaatgt caaggttaaa ctatttaaat gcagtaataa
    16021 tcccattatc atgtaagttt tttatgattg tagctgcgat agggaatcca aataatccag
    16081 ctattccaaa tagttgagct ccaacaaaca tacacattaa tgttattaaa ggatataggc
    16141 caatttggtg tcctactacc tttggttcta atatttgtct aacaacggtt ataattatgt
    16201 atagtattaa aagtcctatt gctaaattaa tatttccatt aatgagtgag ataatcatcc
    16261 aaggtgtcaa aatacttcct gtacccaaga ctggcaaaat atcaacaagt gctatgatga
    16321 cagatatagt aaatgcattt tcaaccttta ggatagtaag tccaataagt agttctatga
    16381 atgtcactga tagtagtatt gcataggctt ttagtagttt taaaagagca ttaatactgc
    16441 tattttttat atttattatc ataaattgac ttttttctgg aaattgtttt aagataaagt
    16501 ttactatttt ttgatagtca aaagtaaaga aaaaagaagc aattaaagta ataataagtt
    16561 ttaacataaa tgcaggtgtt ttacttgcaa tactagcaat agcattgagt gcatttgttg
    16621 aaatagaagc tactaaagac atcatagatt gacttatatt atcccaacca agtattaatg
    16681 ctaggttaac attaggtgta ctagcatcta tctttgaaaa taaagtattt agcgttggtt
    16741 gtatacttga tttatatatt tcaggaagtc taaaaaatac atcacttaca cttgcaaata
    16801 tcttcacacc aaagctaact aataaaaata tacacaaccc ataaaaaatt aaaagtatta
    16861 ctatagaaat aagtgtacga tttaaatttg tattatttgt aataagtctt ataataggtc
    16921 ttaagatgga tgcaatgata aaactgatta caaaaggcat taataatggc aataagaatt
    16981 ttaatacaat atatacaata cttgttatta aaactacata tgtaaattta attataaaat
    17041 tcttttgctt ttgaatatcc ataatataaa acctccaatg tatttgatat tattctaaat
    17101 atggtctttt ttacagaaaa tatactactg tatattgatt ataaactata aagcttatgt
    17161 aaagtaaata aattatgtaa aatttaggtt aattttgtat aaagagatac ttttatattt
    17221 tacaattaat aaccaaatac taaaaaatat tttaaataaa gaacttaaaa ttgtgataaa
    17281 attaatttat agatattaaa aaaagtataa tatatggttt ttaagaggtg ctaagatgaa
    17341 aaattttaga gatttaggtg ggaacaaaac tgaggatggc agaactgtaa agaagggatt
    17401 attttatagg tctgcaaaat tatcaaatct aagtgaaaat gatataaaga ttttaaaaga
    17461 gttaaatatt aaatatatat ttgattatag aagtgatgaa gaagctcgta aacatccatc
    17521 aacaataata tcaaatataa aaaatataag aataccagca atgagagagc tagaagaatc
    17581 aggaggaagt tttggttcta ttgaagatat gattgatgga ttgtttgaaa aagatggtgc
    17641 atttaatatg ttaaataata gttactataa tttgcctata aataatccgt catataaaaa
    17701 attagtagaa cttataagag actattctaa tttaccaata ttaaatcatt gtacagctgg
    17761 taaggataga actggtgtgg ggagtgctat aattcttatg attttagggg tatcgagaga
    17821 gaatataatg aaagattatc taaaaagtaa tgattttgca gataaagaga tagaaagatt
    17881 tatagaatat aagcctaaat ttaaggacat tccaaaagaa aatttgaaat atatatttgg
    17941 tgtaaatgaa gagtatatga aaactgcatt tagaagaatt gatgaagaat acattagtgt
    18001 agaagcatat ttatatggag aatttaattt aaataaagaa gaaataagaa aattaagaaa
    18061 tcaatatcta gagtaaaagg tgagtttatg agtttaaata taacagcaag acagataaat
    18121 ataataaaaa tattgttaaa ttcaaaagat acaattagtg gtattgcctt aagtcaagag
    18181 ataggatgtt cttcaaaaac tatacaaaat gaaataaagg atattaataa acaacttaga
    18241 aatggtaaaa ttttgtcaat aagaggagtt ggttataaac tagaaggaag ttttgatgag
    18301 gtaaatctaa atagcaatct ctatgatgat gtagatagag tagaatatat aatcagaaaa
    18361 atattaacct taagtaatga agaaaaaaat actataaaac ttgaagattt agccgattct
    18421 atgtatgtaa gtgtttcaac agtaaaaaat gatttaaaag aagtaaaaag aatgttggaa
    18481 aagtattcaa tatcagttaa atctaagcat aaacaaggta tatgtgtttt ggaaagtgaa
    18541 gataagatat taaattgtat agttgactta agcaataaga gagataatca attgagttta
    18601 aatgattttt taaatgatga tataaaggaa agaaaactat taataaaaaa attattatta
    18661 gacatattaa atgaaaatgg attagtatta actgatatag aatttaagag tgtgttaaat
    18721 aatatattag ttatgttaag cagacataag tataatgaaa aagaatttat agagaattat
    18781 atagaaaatt ataggagcaa aagaagctca attttagaaa atgaacaaaa caaagaaatt
    18841 ataataaatt caataaaaac attttgtaaa aatttaaaat tagctacatc aatagatata
    18901 agtaatgata aggtatttga agagtttttg tataagcata ttagtagttt atgtaaaaaa
    18961 ataaagttag gtataaatca atcacctata atggctcaag atataaaaat aaaatatcca
    19021 tttgcttttg aactagctaa tatagctaaa aagacaatag aaaaagattt gaagatagaa
    19081 attaatgaag atgaagttgc aaacatagct ctacatatag gaggagcagt tgaaagagca
    19141 tcttataata ataaagataa ggttttcaaa actataatag tttgtacttc aggcataggc
    19201 acatcaatgt taataaaagc taaattagag aatatattta aggaaaaatt agaaatacta
    19261 aagattattc cttcatactt agttgaatat ataaaagtta tagatgttga ttttgtaata
    19321 tctactgttc ctattgaagt aggagatgtg ccagtaataa atatatctcc aattttaagt
    19381 gagaaagaaa ttagattaat tgaaaaatat atagaaacag gaaatatata cctagattta
    19441 gatataaaaa gcctgtttga tagtgaattg ttttttactg atttggtgtt taaaaggaaa
    19501 gaagaagtta tagattttat ggcaagtaaa cttgtacaaa agggctatat agatgaggat
    19561 atgaaaaaat cttactttga gagagaaaaa atagcaacaa cagaaatagg gaatatggta
    19621 actataccac atggagctat tggaaaaatt tttgataata aaatagcaat tggtatatta
    19681 aagaagccta tttcttggga aatatcagat gttcgtttaa taataatgtt agcccttgat
    19741 aaagataaaa tattagatta tgaagtaata ttttcaaaaa tatataaaag agtagattct
    19801 atagcaaaag ttataagtat atgtgaaaat agtagttttg aaaaatttgt aaatttattc
    19861 aattagatgg gtagtattgt atatacacct gtaaatttgg ataatataaa actaaataag
    19921 ataaaaacga taaatttctc ttttaattaa gagaaatttt ttgactttca aggatattaa
    19981 tataaagtta agatataaac ataaagcaaa taaataaaaa agaggtggca atatgattta
    20041 tacagtgaca ttaaatccat caatagatta tatagttaag ctagatgagc taaagacagg
    20101 aagtacaaat cgtgtaaaag aagaatacgt ttatccagga ggaaaaggta taaatgtatc
    20161 tcgcatattt aaggaacttg gaaatgataa tacatcttta ggttttataa gtggttttac
    20221 tggagaatat ataataagaa cattggaaga aaaagaattg aagacagatt ttataaagat
    20281 aaaaagtggt ttttcaagaa taaatgtaaa aataaaggcg ttaaaggaaa ctgagattaa
    20341 tggacaaggt ccaaatatag atgatgaaga cattgatact ttatataaaa aacttgataa
    20401 gctaaatcaa gatgatattt taatattggc aggaagtata ccatctactt tagatgaaaa
    20461 actatacgaa aatataatgg ctagattaga aaaaaagaat ataaaagttg tagtagatgc
    20521 aactaaaaat cttcttctta atgttttaaa atataaacct tttctaatta agcctaataa
    20581 cgacgaatta gaagaactat ttggtgttaa attaaattca attgaagaca tggtaaaata
    20641 tgctagaagg ttaaaggaaa tgggagctat aaatgtcttg gtatcaatgg gaaaagatgg
    20701 agcattgttg attacagagg aagatgaagt gcttataagt gatgttccaa aaggaaaagt
    20761 aaaaaattct gtaggagcag gtgattcaat ggtagcagga tttatatctg ggtatttgaa
    20821 tactgggaaa tatgattatg ctcttaaatt gggagcagct tcaggtagtg caactgcttt
    20881 ttcatatgac ttagccaaaa gagaatacat tgataaattg gtaaatgaga tatctgtaaa
    20941 gcagttttaa aatattaaaa aatatataga aaggtaatta atatttatga aaattataga
    21001 tttattagat gaaaagtcaa ttaagttaaa tttaaattca aaaaccaaat cagaagcaat
    21061 agaagaatta gtagatttgg tcgcaaatag tggaaattta aatgataaag aaaactacaa
    21121 aaaagcaatt ttggctcgtg aagaaatgag tacaacagga attggagaag gtgtagctat
    21181 acctcatgct aaaaattctt cagttactaa agcttgtata gcagcagcag tgtcaaaaga
    21241 aggtatagat tatgaatcgt ttgacggaag tttatcaaat ctatttttta tgatagctgc
    21301 accagacgga gctaacaata ctcatcttga agtattatca agattatcta caatacttat
    21361 ggatgaagac tttagaaata aacttataaa ttcaagctct gaaaaagaat ttaaggaaat
    21421 aatagataaa aaggaaagag aaaaattcag tgaagaatat caagatgaga aagttactga
    21481 aaaaaatata atagatacta aagagaatga tgctaataag tatcctaagg ttttagcagt
    21541 aacagcatgt ccaactggaa tagcacatac atttatggca gctgaaagtt taaataaaat
    21601 ggcagaaaac aaaggtgtat caataaaagt agaaactaat ggttcagcag gtgttaaaaa
    21661 taagcttaca aaagatgaaa tagaaaatgc aacatgtata atagtggcgg cagataaaaa
    21721 tgttgagatg gctagattta atggtaaaaa agtcataaaa actaaggtgg ctgatggtat
    21781 acacaaagcg gaagaactta tagacaaggc tgtaaatgga gatgctccaa tttaccatgg
    21841 aggagatgga agtcacaacg aatcaaatga agaaagtgaa agtggattta gaaaagtata
    21901 caaacactta atgaatggtg tatctaatat gttaccattt gttattggtg gagggatact
    21961 tatagcgata gcctttttac ttgatgatta tactattaac ccttcaaact ttggttcaaa
    22021 tacacctata gcagcatttt ttaaaggaat aggagataaa gcttttggat ttatgttacc
    22081 tgtacttgct ggatatatag catactctat atctgataga ccagcgtttg ttgtaggatt
    22141 tgtaggaggt gctctagcag gtgatggtgg ttcaggtttt ttaggtgcac tacttgcagg
    22201 ttttatagca ggttatttag tagaagggct taagaaaata tttagtgtac taccagcttc
    22261 attggaagga ataaaaccag tattgttata ccctttactt ggaacacttt tgatgggaat
    22321 tataatgaca tttttagtaa tacctccagt tacagcaatt aataatgcaa tggttggatt
    22381 tttaaatggt ttaggtggaa cttcaaaaat agttttagga ttagtattag gtggaatgat
    22441 ggctgtggat atgggtggac ctgtaaataa agcagcatat gtatttggag ttgcatcact
    22501 tgaatcaggg cagtttgaaa taatggcagc agtaatggct ggtggtatgg ttccgccact
    22561 tgctatagct cttgcaacta cattttttaa gaatagattt actaaagaag aaagagattc
    22621 tggtaaagtt aattatgtaa tgggattatc atttgttact gaaggagcta taccttttgc
    22681 agctggagac ccacttcatg ttataccagc ttgtgttgga ggttcagcag tggcaggagc
    22741 acttagtatg ttgttcaacg cagctcttcg tgcacctcat ggaggagtat ttgttatacc
    22801 agtggttacc catccatttg catatatatt agctatagct gtaggtgctt tggttggaat
    22861 gatgctttta gcattactta aaaaaccatt aaatcaagaa gtatagcaat taaaggatat
    22921 tcaagaactt aaaatatgat gttttataaa aattaatata ttgctgaatt attttaaaat
    22981 aaggatttta tgaggctgtt atataacgat ttgtaattgt tatcttgaca gtcttttatt
    23041 tttatataag taaaataagt gtgttttgct ttattt
//
LOCUS       DAYATN010000052        22937 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.052, whole genome shotgun sequence.
ACCESSION   DAYATN010000052 DAYATN010000000
VERSION     DAYATN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.052"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(239..1282)
                     /locus_tag="ACGI8A_001908"
     CDS             complement(239..1282)
                     /locus_tag="ACGI8A_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179905.1"
                     /translation="MQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEVKQ
                     EMTVMKEDMSEVKQEINVLKEDMSEMKQEMTVMKEDMSEVKQEINVLKGDMSEVKQEM
                     IVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEMTVMKEDMSEVKQEINV
                     LKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMK
                     EDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMKEI
                     QIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATS
                     KNWSDIVKLKSVK"
     gene            complement(1518..2603)
                     /locus_tag="ACGI8A_001909"
     CDS             complement(1518..2603)
                     /locus_tag="ACGI8A_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFH0179906.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2639..3952)
                     /locus_tag="ACGI8A_001910"
     CDS             complement(2639..3952)
                     /locus_tag="ACGI8A_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0179907.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(3953..6244)
                     /locus_tag="ACGI8A_001911"
     CDS             complement(3953..6244)
                     /locus_tag="ACGI8A_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFH0179908.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6234..7028)
                     /locus_tag="ACGI8A_001912"
     CDS             complement(6234..7028)
                     /locus_tag="ACGI8A_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFH0179909.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMTKALVVRMYNSIGGECDGK"
     gene            complement(7067..7513)
                     /locus_tag="ACGI8A_001913"
     CDS             complement(7067..7513)
                     /locus_tag="ACGI8A_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFH0179910.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7585..8991)
                     /locus_tag="ACGI8A_001914"
     CDS             complement(7585..8991)
                     /locus_tag="ACGI8A_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0179911.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     IRGTIKNRMEGHKWNSLY"
     gene            complement(9009..10379)
                     /gene="hydA"
                     /locus_tag="ACGI8A_001915"
     CDS             complement(9009..10379)
                     /gene="hydA"
                     /locus_tag="ACGI8A_001915"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HFH0179912.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKIAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10512..11699)
                     /locus_tag="ACGI8A_001916"
     CDS             complement(10512..11699)
                     /locus_tag="ACGI8A_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFH0179913.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            complement(11826..13040)
                     /gene="dpaL"
                     /locus_tag="ACGI8A_001917"
     CDS             complement(11826..13040)
                     /gene="dpaL"
                     /locus_tag="ACGI8A_001917"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFH0179914.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            complement(13403..14074)
                     /locus_tag="ACGI8A_001918"
     CDS             complement(13403..14074)
                     /locus_tag="ACGI8A_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0179915.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(14157..16373)
                     /locus_tag="ACGI8A_001919"
     CDS             complement(14157..16373)
                     /locus_tag="ACGI8A_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFH0179916.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(16376..16846)
                     /locus_tag="ACGI8A_001920"
     CDS             complement(16376..16846)
                     /locus_tag="ACGI8A_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HFH0179917.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(16908..18302)
                     /locus_tag="ACGI8A_001921"
     CDS             complement(16908..18302)
                     /locus_tag="ACGI8A_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0179918.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(18375..19736)
                     /locus_tag="ACGI8A_001922"
     CDS             complement(18375..19736)
                     /locus_tag="ACGI8A_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFH0179919.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            complement(20033..21355)
                     /locus_tag="ACGI8A_001923"
     CDS             complement(20033..21355)
                     /locus_tag="ACGI8A_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFH0179920.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            complement(21711..22751)
                     /locus_tag="ACGI8A_001924"
     CDS             complement(21711..22751)
                     /locus_tag="ACGI8A_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0179921.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT         6822 a         4639 c         2526 g         8950 t
ORIGIN      
        1 attgataaaa ttttacttat ttcaagcgtt ctattagatt ttatcatcta aattttaggt
       61 tttattttta taaattaatt gtatttacat aaaaaaaaag acctaaaaac aaatttttaa
      121 cggtcttttc tcaatttttt attttcattt ctaaaattta taataaatag taagaacact
      181 ataaattctt ttatataaaa ttcttagttt gtggatgata aattagtgaa ttaaaatact
      241 actttactga ttttagtttg acaatatcac tccaattttt actagtggct aactctacta
      301 aatttaagtc tcttcttatt tcagctatat cctcttttat agaattagtg tcacctttta
      361 tatatgctat gttatgttct atattatcat gagtagcttt atttatttca gaattatgta
      421 ataaacttcc taaaatatta ttctgttcta tctgtatctc tttcattgat ttatcttcac
      481 attctagact atcaatctta atttcaattc catataattt ttcatttgct atggatacct
      541 cgtccttaat tccattcata tcttcattaa tgttatttat tttattttgc ataaagttta
      601 cttctgattt aacttcggaa acatcttctt tcattacagt tatttcttgt ctcacttctg
      661 acatgtcttt tttcattaca tttatctctt gttttacctc tgatgtatct tctttcatta
      721 cggtcatttc ttgtcttact tcggacatat cttctttcat tatatttatc tcttgtctta
      781 cttcggacat atcttctttt aatacattta tctcctgttt tacttccgac atatcttctt
      841 tcattacagt catttcttgt cttacttccg acatatcctc tttcattgta tttatctctt
      901 gtcttacctc ggacatatcc tctttcatta tatttatctc ttgtcttacc tcggacatat
      961 cttctttcat tacaatcatt tcctgtttta cttctgacat atctcctttt aatacattta
     1021 tttcctgttt tacttccgac atatcttctt tcattacagt catttcttgt ttcatttccg
     1081 acatatcttc ttttaataca tttatttcct gttttacttc cgacatgtct tctttcatta
     1141 cagtcatttc ttgttttact tccgacatat cttcttttaa cacatttatt tcttgtttta
     1201 cttccgacat atcatttttt atgctaacta catcttcatt tatagtatat aataactcta
     1261 aaatcttctc ttgcacttgc atcaccatcc ctattaatta ttataacatt ttacacaaat
     1321 gctatacaat gttaacatat atatcagaat tattaaaatt ttaattaaac aaaattgatt
     1381 cactttatta cttcaaaact aattaatatc taaaataact acaaataaac aaaaataagg
     1441 tcaacaacaa aaaaggctta tctctttgag atagcctttc ttatttttat ttaaaacttt
     1501 gttttttata aaataatcta tacattcagc tctgaacatg aaatttttct tccaagtgct
     1561 tttggtttac aaactgatat acaaagtcca caatatctac actcttcttc attcaattcc
     1621 atcttatcat tcaattttct cgcttgatat gcacaaactc tctcgcacat cttacattta
     1681 gtacattttt cttcatcata agtaaactca aagctaagtc tttcattaac atctcccata
     1741 tgattatgta ctataccaca agcattttct atagtattat atcctaatct attaagatta
     1801 gattttaaat catcatgtat tttagatatt acttgtgcac cttttaaaat tactactgaa
     1861 catataccaa caaagtcagc acctgccata atcatttcta aggcatcatc tccacttaca
     1921 catccaccta agccaattat tggtatatta acttgttttt ttatttcata tattctctgt
     1981 aaagttattg gcttgatagt ttctccactt aaatatccta ttccatttcc acctaataaa
     2041 ggttgccctg tttctatgtc tattctaaat gctggtccta cagagtcaca agcagttatt
     2101 gcatctgctc ctgcttcttc taatttttta gcaaaatcgc ctatctcatc tatcatagga
     2161 ggaagtttaa ctattacagg tatatttact ctttcctttg catctttaag cattggtatc
     2221 aaatctctat agtcataaga tactaattca ataaagtcag ctcctgcatt tgcaactttc
     2281 tctactaatt cgctactttc ttcaattgta tgacctattg aagctataca gacagtccca
     2341 tctcttttca tttgtggtat ttcaaaatct atccattgct ctggctcaaa atctgtccat
     2401 ttttcattat ttaaaagagc attagctgta tttctaacca tatgtggtgc tggatttata
     2461 gctctttctt ttcttatagt cttagtcaca actgcccctg ctcctgcatc acttaatatc
     2521 ttgcatccag ctgcactata tgttaatggt cctgaaccta ttataagtgg tgattttaat
     2581 tctttaccta aaaaattata cattatctgc ttcccctttt catatatatt tacattcgtt
     2641 aaaatttcca aaattcacaa gcaacttctt tcatttcttt aacaatagct tttttatcta
     2701 aatcaactaa ctgtttttct tttaaaatat gtctaccatc tatgtataca tttgatataa
     2761 attctttttt accctgaact actatctggt caaatatatt atcaagagtc acaggagttt
     2821 taaattcatc ttccataact attatatcag ccttattacc aactgctatt ttaccagaat
     2881 tttgaagtcc taatacataa gcaccatgct ctgttgccat tctaaatact aaatttgctg
     2941 gcattactga tgcatcttct tcaactgatt tatgcattaa aaatgcacct ctcataactt
     3001 caaaaaaatc atttatatat ccatcagtac ccagtgccac ttttatgcct tttttaatca
     3061 tttttggtac tggagaaaaa cctccaccta cttcacagtt actaagaggc atatgaacca
     3121 cttttacgcc tttttctttt aatatatcta tttcttcatc atttactttt acacactgag
     3181 atgctaatac agtttcatcc aatatatcta aatcattata gtagtcaact gcttttttgc
     3241 caaagtgttt ttcagcatag tttggctcat atatactctc acataaatga aattgcattg
     3301 gtgcatttaa tttttttgca tcttctttag ctttcttaat aaatctatca gaacatgtga
     3361 agcttgtatg tgtacacatt attccattta ttaatttact attttctcta ctccacttta
     3421 ttaaattaga attttcatct aaacatctaa gtccattttc aaagcttatt ctttcacaac
     3481 tttctaagga taaaatcgct ttcatgccta tttcttctag tatttttcct tgctcaatta
     3541 aagttccttc ttctgtattt ggagcttcta gggtatcaca aaaagctacc acaccagatt
     3601 ctataagttc tattgctgat gctttagttg ttgccttcac ttcttttaat ccaattctat
     3661 tttctataaa tggccaccaa tagtcattta aaaaaccttc aaagtcagta aaatgtacat
     3721 ttgccggtat tcccctagaa agaactccat attgatgcat atgaccattg acgaatcctg
     3781 gcattattat agcatctgat ttatctatta tttcttcaat atttttatat tttatttcaa
     3841 cttcttcatt tggcaatata tcttttataa tgtcattttc tactacaaca gcagaatttg
     3901 tatatatttc atcagcagaa gatattaagt atttactttt aatagcaatc atttatttac
     3961 ctccctgatt taattttttc atggccatat aaactttctc agaagttata ggaagttctc
     4021 ttatacgaac tcctgtggca ttgtatattg catttgctat tgctggtgca ggtgtatgaa
     4081 taactatttc tcctaaagat ttagctccat atggacctgt tggctcataa ttatctacaa
     4141 aatcaacaat tatgtttcct atatcttttt ttgttggaac tttgtattgt aatagattag
     4201 aagtttgaag ctttccatca gaaccatgtc tagggtcttc atacattgca agccctatac
     4261 cttgagttac accaccttca acttgtattc tagcaagtgc aggattcata actgtaccac
     4321 aatctgtaga acaagcataa tttattactt taaactcccc tgttgtttta tctaactcta
     4381 tttcagcaaa tcctgctaag aatggtttag cactctcttg tataccataa gattcagatg
     4441 aatataaaac ttcttgattt ccaccaccaa cagattcttt tcctaattca cttaacaaaa
     4501 caaacttctc tacatcttca ctataacaaa ctctgtcttc atatagtatt aaatcatcta
     4561 ttggtaaatc gagtttcttc tgtgctactt ttaatatctt ttgttttaaa ctttcagcag
     4621 attttattgt agcatttcca gttacataag tagtacaaga agcgtatgca ccagtatcat
     4681 atggacacat atctgtatct cctgtataaa cggaaattct atcaatagat gtattcaatg
     4741 cttttgcagc tatttgtgca agtattgtgt cagaaccagt tcctaagtct gaagacccag
     4801 aaaacaactt ataagttcca tcttcatcca tcctcatatt tactattgcc atgtctatgt
     4861 tggctatacc agaaccatga gttcccacag acattccaac agccctcacc ttattgtttc
     4921 caatatccct aacagggtac ttttcatccc atccaataag ttttttacct ctaattatac
     4981 attcatcaag tgcacaactt cttattggaa aattcattat tccgccttca gtttgttttt
     5041 ttataatatt ctttaacttt atttctgttg ggtccatatt aagtttatgc gctaattcat
     5101 caattgcaca atcaagtgca aatgtacctt gtgttgctcc atatcctctt aatgctcctc
     5161 ctggaactaa attagtatat acagttttcc catcaaattt tattgcaggt acattattat
     5221 atgttggaag tacattatga cctgattcag agcaaactga aggtccatta tcgccataag
     5281 cgcctgtatt gttcaatgct ttcatatcta tagcttttat gtttccttct ttatctgccc
     5341 caatttttat atcaaaaaac atctgatgtc tagtattagt cattgcaaaa gtttcttctc
     5401 tagtataaat tagttttgct ggtctttttg tcatccaagt tacaaatgat gcatatatct
     5461 ctgttacagc tatgtttttt ccaccaaaac ctccacctat tcttggtttt attactctca
     5521 cttttgaaag aggtattccc agtgctttag aaacctgtct tctcatatga taagctgatt
     5581 ggtttgcaga agttactact aatctgccat tcgtatctat atacgtataa gctctatgag
     5641 tttccatcat acaatgggct tgtgattgag tcatataaga atgttctact attacatcac
     5701 tttctttaaa ctctttttct acatcccctt tacccatttt ttctctcgat acaatatttt
     5761 ttgtagcatc caatccaaaa tcaaatggac aaaacaaatc atcttcttca tggattaaaa
     5821 ttttatttcc ttccgattct cttgcatcca atataggagt cattacttca tattcaactt
     5881 ttataagttt catagcttta atagcagccc tttcatcttc agcggctatt atagcaactt
     5941 catctcctac atatctaact acgtcttcaa gtattaattg gtcatgaggt gatgcttctg
     6001 gatatgactc tccaaccatt gaatatctat aatttggaac atctttgtaa gtgaatatgt
     6061 ttactacacc ttttaccttt aatgcatttt ttatatctat gttttttatc ttcgcaaaag
     6121 catgtggact tctaagaagc ttaattgtta aaacatcttt atgaaaaatt aaatcttctg
     6181 tatatattgg ttttccagta acaatggctt cactatctat ttttcttacc tctttattta
     6241 ccatcacact cacctccaat actattatac attctcacta ctaaggcttt tgtcatatct
     6301 tttctgtatt ctttacttcc tcttatatta cttccaaatg aaagttcttc acttgatact
     6361 tcaccagctt tttctatatc attttctaaa cttaatattt cacttgcttt ataggcaatt
     6421 tttgctcttc ttggtctagc gcctatcgca attttataaa tacttccttc ttttaacatt
     6481 gctccattta atactgcaaa atcacctgta cattttctta tagaatcaaa tacacctata
     6541 gcatcttttt taggtaatat aacttctatt aatatatctt tttcaagctc attttctaaa
     6601 aaatcagcta agttcataac ccctttttta tataacttaa cctttgcatc taccactagt
     6661 aatgaaggta ttaaatctga aaatccatac ttagcaaata cgctagctcc tactctagct
     6721 ccacttctaa attgaactcc tactatatta gaaacagcgt ttgaaattac cccattacag
     6781 taatttttaa taaccttatt taattctaaa gttcttaaac ttgtatctgc tccaattaat
     6841 atgctatcat cagtttcatt tatataatct aaagctaagt cttttaaatc tattgctgaa
     6901 ccaatatttt tactacctag ttttaaaaat ccacatcctc caagtattat gttgtttttt
     6961 cttgatatta gcagttcata agcttcatct aaactttttg gaactacata ttcctttata
     7021 gttatcatac tctctcccca tatcaactta attttcactt aaaagtctaa gtgtttaaat
     7081 atttatgtat tgctcttaat tgacctttat atcctgtaca tctacaaagg ttaccattta
     7141 cataatctat aacttcttct tctgttggat tttgaagctc tcttttcatt gccaagacca
     7201 tcattataaa tccaggagca caaaatccac actggtcagc cccttcgtct gcaagaaaat
     7261 ctccaaactt ctttgcttca tccaagacac cctcaagtgt tgtaatatgc tgaccttcta
     7321 ctctagcagt aagataatta catgaaagca cggccttgtc atttaccatc actgtacata
     7381 acccacatga gcctgtatca cacccttttt taacacttac atatccattt tctcttaatg
     7441 tatctgctaa aaaatcatct ggtcttatac ttaattcttt ttctttatta tttatggtac
     7501 atttgataat caaataaatc acctcaaaac tcatattttt aagctaaagt tttactatac
     7561 ttttttatga ataaattaca tttattaata aaggctattc catttatgac cttccattct
     7621 attttttata gttcctctta tacaaagtct ctcagctgta tcttgagcat cttttaatgc
     7681 tttctcttca ttagctgtca atatttttga atcttccatt attatatttc catctactat
     7741 aacactctct atatttttca tactagaaga atatactaat gtagataccg gattatgcat
     7801 aggtatcgct tttggtgaaa gcattggatt aaatattagt aagtctgctt ttttacctat
     7861 ctccaatgaa cctatctcat cttccattcc aatagctctt gctccatcta tagttgccaa
     7921 ctcaagaact ttttctgccg acattgctaa tgggtcacac ttattaactt tatgttgaag
     7981 tgctgttaat ttcataagtt caagcatatc ttgagagtta ttactagcgg ctccatctac
     8041 tcctaaacta actgttattc cttttttaag catttctggc actggtgcaa cacctgaaga
     8101 taagtacata ttacttgctg tattatgaga cactttcata tcgtattttt tagtaagttc
     8161 catatctttt tcagttagat acacacaatg taccataagc acatttggtc caagtattcc
     8221 taatttttct aaaacatcta tgtcatattg cccatgcaat tcttttgctg cttctctatc
     8281 aaaaggagtc tctgatatat gtactgtaaa taaagcatca tcatattctt ttactactct
     8341 ccatagcatt tcaagcattt cttgagaatt tgaccatatt gctgcaggag ctactcctat
     8401 tttgattctt ccattttctg tattatggtg tttttcaaac aatcttctta catctttttc
     8461 aaccgtttct acatcttgca ttattccagg atgaactcca aattgagctc ctgtattcat
     8521 acatcctctt ccaagtattc ctcttattcc taattcttta taagcatcta ttattccatc
     8581 acaaagacct ggcttactat gaggatacat gtagtcaacc attgttgtta taccacttct
     8641 aagtccttca atacatccaa gcattgcagc atcataagta tctttgggct ctaaatagtt
     8701 agctgctgga aatgtcatag tttctaacca gtctttaagt accatatcat ctcccaatcc
     8761 ttttaataaa gtttggaata aatgattatg tgtatttata aaacctggga ataaaacttt
     8821 acctttagca tctataattt ttttaacatc agtatattta ctttctatct ctttagaatt
     8881 tccaatatca gcaatcttgt tatcttttac tactaatgcc ccatcaaaaa taacttctct
     8941 ctctttattc acagttacta ttatggcatt cttaataagt atatccatca ttcttatttc
     9001 cccccgcatt atttagtaat ttttctttta atatattgac ctgttggatg ttctttaaca
     9061 ttacaacttt gtactacatg atttcctctc aaaaatacat cttcaacttt acatttaacc
     9121 ttaaatcctt catatggagt ataatctaca ttttgttttt gagttttata actaattgtt
     9181 tcttctttat tgatatttaa aatgactata tctgcatcac ttcctacagc tatctcacct
     9241 tttttaggat acattccaaa tatttttgca gcattcgtag aagtaacttc cacaaactta
     9301 tttacactta ttctattttc taagacccca tatgtatata aaagtgctaa cctatgttca
     9361 acaccaggtg ctccatttgg tatcttactg aaatcatcaa ttcccaagtc tttttgcccc
     9421 ttaaaattaa atgaacagtg gtctgtgcca actgtttgaa tatctccact agacaatcct
     9481 tcccaaaggt aatttatgtc ttcttttttt cttaaaggag gagacattac atacttagca
     9541 ccctcaaatt tatccttatt atataaattg tcttctaata atagatactg tgggcatgtt
     9601 tctactatca tatctatatt tttttctcta gctattttta cagtttcaag tgactcccta
     9661 caacttaagt ggactacata ggattttgaa ttagataatt tagttatatc agcaagtctt
     9721 gaaacagact ctttctcaac tatgttaggt cttgtaagtg gatgatattt tggctccaaa
     9781 ttaccatttt gtatattttc ttctatcaat acatctagta aatccccatt ttcacaatga
     9841 aatccaacta tacaacctaa atcccttgct ttctttaaaa ccctgtatat tgttccatca
     9901 tctactttca tgccatcata tgccatatac atcttaaaag atactatccc atctgaaata
     9961 agtttttcca tatgattaaa tgtatcttta tttaaacatg taattgtcat atgaaaacca
    10021 taatcacaat aacaatttcc acttgctttt ttatgatatg cttctacacc ttgcagtaga
    10081 ttttccccct cattggcttc tgcaaaatct agtattgtag tagttcctcc agctatagct
    10141 gctcttgttc ctgtttcaaa attatctgca gttgtaatag aacctgcatt catatcaaaa
    10201 tgagtatgtg tgtctattcc accaggaatt acaattttac ctgtagcatc aataacctta
    10261 tcaccattta tttctaagtt atttcctatt tcaactatct tttcatcttc tatccttaaa
    10321 tcacttatat aagtcttatc agaggtaatt atagttccac ctcttagtat tgttcccata
    10381 tatacctcct aacttttaag ctttaaagta ttttctaagt attgtaaaac tttatttaaa
    10441 attgtatttt ctaaaggtgc tatattcagc acctctagac tatatttaaa taatttattg
    10501 tgaactaaat attattttcc gtatacagat tttaatattc catagtaacc ttctgttcct
    10561 ataaataact gttcttgctc tatatactca tctattgtgt gagcaagatt ttccttagaa
    10621 ggaccaaatc ctatagtttt tattccagct tcacctgcat agtgacttcc attcgtacag
    10681 aatgagtatt gagttatttc tgaatctatt ccagcttctt ttaatccctt atacgctgct
    10741 tgaacaaatt catcttcttc gtcatataac caacctggga agaatctttc actttctata
    10801 gtatttcctg tatagcaatc agctttttca accgcataag aaacttttgc atttaattct
    10861 gggtcttctt tcatcatttc atctaataaa gcttgtattg gagctaatac gccttctctt
    10921 gtttctccaa ctaataatct tctatcaaaa gtagctttgc aataatctgg cactactgat
    10981 gctcctggat atggagaaga ttttatatct gttaaaacta atattccatc acctaaaact
    11041 ggatgagttg gaggtactaa ttgttgtatc ttttggatta cattagccat tttataaact
    11101 gcatttacac ctttttctgg attagcagag tgagctggtt taccaaaagt ttctactact
    11161 atttctcctc tacctctctg accaatcttt aagtttaact ctgaagactc acctattaca
    11221 acatagtcag gttgaacagc cttacttatc tctctagccg aaacaccttc aaatatttct
    11281 tcatgaacaa cacctgctac atatagttct ccagcaaaat ctctattagt gtctttagca
    11341 aaatacgcta cagcagacat cattgcagta tattgacctt tcatgtcaga agttcctcta
    11401 ccatatatct ttccatcaac tatttttcct tcaaaaggag gaacactcca tttacttgca
    11461 tctggaactg gaactgtatc tatatgtcca tcaaataata ttttcttacc tggcttgtta
    11521 ccttttatac atcctactat atttccatat ctatctcttg accaactatc aaaacctaat
    11581 ttttcaaaag actcttctat tgcttttaca acaccttctt catttccaga agaactagga
    11641 ttttgaatta atttttggca tatttcagtt acttcttgtt ttctttgttc gtttaacatt
    11701 tcattatccc cctagatttt ttataatgta tgtattttat atacttatat attttctaaa
    11761 ttttagaact tatatatctt ctaaatatgt aaaccaatat ctaataattg cattttagta
    11821 attccttata tacttggata ttctccatcc caaactatac ttctatattt gtcagggtca
    11881 gtatctcctt ctgtgcttat taaaagtatt cttgaatttt catctaattt taaatctttt
    11941 cttaaatctg ctaaatcttc tctttccata gctgctacta aaagaccagt tgtaactgct
    12001 ccagattctc cagaaattac cttttcatca ccatttaaag gattacctaa tgttctcata
    12061 cctttagcag aaacccagtc tggtgctgaa acaaatgctg ctgcatgatt ttttaatact
    12121 tcaaatccta tagtatttgg ctctccacaa gcaagccctg ccattatagt tggcatatct
    12181 cctccaacaa atcttggttt tccatctcca gcttctgctg atttatagta acaatcagct
    12241 tcatcagcct ctactactac tgttattggg cattcatcat atattgaagc aacataccct
    12301 tgaactgcac cagcaagtga accaactcct gcttgaacaa atacgtgtgt tggtctatct
    12361 actccatatt cttttaattg ctctatagct tcttgagcca tagttccata tccttgcatt
    12421 atccatgctg gtatttcttc atatccgtcc catgctgtat cttgaaccat tactccatgg
    12481 tcatcagctt ctgcataatc attagctaat cttactgcat catcataatt taagtctgtt
    12541 atactagcat ctgcaccttc tcctttaatt ttatttaatc taaattcaga agaacctttt
    12601 ggcattaata caacagattt ttgacctaat ttattagctg tccaagcaac tcctctaccg
    12661 tgatttccat ctgtagctgt aaagaaagtt atttctccta atttttcttt tatttctttc
    12721 gaagtcaatt tgtcataccc taactcagat atatctgtat ctaatctttg agctaagtat
    12781 ctacccatag aataagaacc acctaatact ttaaatgcat ttaatccaaa tctataagat
    12841 tcatctttta catatactcc tgcaacacct aatttttttg ctaaaccttc taggtttgct
    12901 aatggagttt ctttatattg aggaaagctt ttgtgaaaat ttctaacctt agttatttct
    12961 tcttcattta aaaatttgat acaattttcc ttatcgccct ttggcaaatt atttactttc
    13021 catttaattt cttttaacat ctaaaatacc tccaaattat tgttaaattt ttatacttta
    13081 atatataagc aattaacatg ccaatactaa atatatacta gatatttttc catgactctt
    13141 tagtatagaa attgcaatgc ttttattatt ttttcttttc tatctttagt tcatacaatg
    13201 tatgtagaat ttaataacta aaaaaccttt tctatcttct cactttttaa ttctcatttt
    13261 taaaatttaa aaataaatct caaaacactt tgtttctata ttttttaata taaaatttat
    13321 aaaatctaaa ttaaaacata aaatagtgat gcaataatct attacatcac tatttgtata
    13381 tattcaataa tataataaat ctttagtggt ctctaagagg tgatttttca tcttttttaa
    13441 catctaattc ttcttttacg acattccatt tttcaagacc aaacttagaa accataactg
    13501 cctctgctgc accttttcca agtcttttta ctgtgtccat acctgtctca tcctcttggg
    13561 caccttttgc ttttccatct ttactccata ttgtaccacc tatatagcat ccacctaaac
    13621 ctccagacac taacatttca tgcattagga agaaatttat tataggaagt tttgtcaatt
    13681 cttggccacc atatctagtt ccaccagtag ttatagctgc accaacctta ttttgaagtt
    13741 ttcctggatg aactaaatac attggacgaa gtctattaaa tactgctgta agttgagcag
    13801 ttatattcat atcataaact ggagaaccta ctataattcc atctgcctcc aatatttttt
    13861 gctctaattc ttggatatca tcttttataa tacacatagt ttcttttctt atacatgcat
    13921 cacaatgtac acaaggactt atttttttcc ctctaactga ccaatactca gtttctatac
    13981 caggtatagt ttctgcttct ttcaaagctt ctaacactgc atattctgtt gctcctttac
    14041 gtggacttcc acaaatacct aatatctttg ccataataat atctcctcac caaataaatc
    14101 tatttttatt atctatttac aaatacaata aattgttatt tctgctttaa tttctactat
    14161 tttaatttta ttggtaatga atgtatctct acatcagttg cattatatac tgcatttgca
    14221 acagctggcg caactggtac aacggcacat tctccaatac tcttaccacc aaaaggccca
    14281 gctttttcat aaccatctac aaatgcaact tgacattttg gcatatctac agttctaaat
    14341 aatttatatg ttttaaaatc agtagcttta ggtctacctt gttcatcaaa tgccaattct
    14401 tcactcaatg cgtatcctag acccatatga atcccacctt ctaattgtcc ttctaaattt
    14461 agaggattta taactctacc aacatcatgt acagctacaa aatcaactac ttttatatcc
    14521 ttagtttctt tatctactaa aacttcagca aaatgtacac cataagaagt acgtccagct
    14581 ggtgaagaat aatcttcaac aacagttaac tctcttaatg atttactttg actatatact
    14641 gcgacatcag aaagtgatgc ttttacattt tcatcaagtt tactaattac acatccattg
    14701 tctagatata aatcttcttt tgatgtttca agtaattttt ctgcttcttc taatataaga
    14761 tttttcaatt tctctgctgt ctttttagct cctccacctt ctacaaatac ccctctactt
    14821 gcatagtcac caaggttgaa tgtacatgca tccgtatcag tttcaaatgt atcaacttta
    14881 tcaggtgtta ttcctaaaac ttcagctacc atcattgttt gcatagttac aacaccatta
    14941 cccatatcat gagctcctgt tagtagtgtc aacgtaccat cttcatttaa tttaagagtc
    15001 aatgtaacaa catctctatg tgcaccaaaa actccattac catgtgcacc tattgccata
    15061 cctatacctc ttaaatattt tccttcttct tttggaagtt ttttcttttc atcccactta
    15121 aacatcttct taccttgctc tatacaatcc aatactctag gatttccaag agaaccgtgg
    15181 aagattatat cgacatcatc tggctctact gcatttttat tttgaaaatc gactaaatcc
    15241 atacctattt cctttgctat tttcgcaaat tgagcttgtt gagccataaa tatttgaggt
    15301 gacccatagc ctctcatagc tcctgctatt ggtgtatttg tatatacagg catcccagta
    15361 aactttatat ttggtatttt ataaacttta aatactttat gactaagtgc tcctataaca
    15421 tttaaagcac ttgatgcata tgctcctgta tttgtatata tttttatgtc ttgtgctatt
    15481 acttcaccat ctttatttac accagttttt aattttacaa aagacccatg acgagttctt
    15541 gttgatatca tagattcctt tcttgtaaga accattttaa ctggtcttct tgtcattttt
    15601 gataatgcgg ctgctactgg ttcaattgta agttctaatt ttccaccaaa tgagcctcca
    15661 atagcaggtc ttataactct tactttattt aatgacatcc caaaaatttg agatagtatt
    15721 attctaaatc caaatgtatt ttggcaaggt gtatgtacag ttaatttatc attataatca
    15781 tagtgtgcaa ttacactatg agtctctatt gcactatgat gtattgctgg agtagagtat
    15841 tcatcttcaa atatataatc acattccttg aagccttttt ctacatctcc cccatttatg
    15901 ttgttttgag aaactatatt tgaatctcca tgtatgcaga tagcatcttc ttttattgca
    15961 tcttgtatag taaatacagc tggcaattct tcatattcaa cttttataag ttttacagct
    16021 ttctctgcta tttctggagt ctctgcagct actgctgcaa cacggtcacc aacaaatcta
    16081 actgtatctg aaaatataac ttcattttca ggtattttgt gctcataaaa tcttaatgaa
    16141 ccattgtatt ttctttgtga tgtattgaga taagtagcta tagctctaac acctactact
    16201 ttctcagctt cacttgtatc tatacttttt atttttgcat gtgctacagg gcttaaaagc
    16261 atcttagcgt aaagtacatt ttgtggtttc atatcagcca cgtatttcat ttgaccagta
    16321 acttttaaag ctgcatcttt tagagggtat gattttccta ttaccttttc cataattatc
    16381 taccccctaa tctagctgca gataatttta cagcttctat aatctttaca tatcctgtgc
    16441 atctacagag attttcttta aaagcttctc taatttcatc ttcattagga gatgcatttc
    16501 tatttaaaag agcttgtgtt gttataatca taccaggagt acaatatcca cattgtacag
    16561 ctccgacttc tataaaagct tcttgtattg gatgaagatt ttctccatta gcaaaacttt
    16621 ctatagtgta tataacttta ccttcgcagt tttttgcaag aagtttacaa gatttcatgt
    16681 caactccatc tatcaaaact ttacatgctc cacaactgcc actattacaa ccttgtttcg
    16741 ctccagttag atttaatttt tcacgaagta catgcaaaag agtttcattc ttttcaactt
    16801 ctatttcata catgtaatca ttaatattta attttatttt ttccattttc tcctcctaca
    16861 gataaactaa tgttgtaata gaatattatt acaatcttat tttaatatta gtataagcta
    16921 ttccacttat gtccttccat tctattagtt acattagctc tcttacataa ttcttctgct
    16981 gcatgttgtg cattaagcaa cacttgttct tcactttcaa ttgtagttac ttttccattt
    17041 tcaagtatta catttccatc aactaaaaca ctctctacat tgtgcatact agctgagtat
    17101 accaaagttg atacaggatt gtgaagaggt attgattttg gagaaagcat tgagttaaat
    17161 attactaggt cagctttttt cccaatctcc aaagaaccta tttcatcttc cattctaaga
    17221 gttcttgctc cctcaattgt agccatttca agaactttct ctgcagaaat tgccagaggg
    17281 tcacaattat ttactttgtg ttgtaaagct gttaatttca taagctctac catatcttgt
    17341 gcgttattac ttgctgcacc gtcaagacct aaactcacat ttataccttt ttttaacatt
    17401 tctggcacag gtgcaacacc agaagcaaga tacatgttac ttgctacatt atgagaaact
    17461 tttatgtcat actttttaag catttccatg tcttcatcag ttatacatac acaatgtact
    17521 gctacaactt ctggacctac tataccccat ttttcaagta attttatatc tatttctcca
    17581 tgtatatcct ttgttgctgt tcttgcaaat tctgtttcag atatatgaac tgtaaaatat
    17641 gaatcatatt ctttaactat attccaaagc atcttaccca tttcttcaga tatagcccac
    17701 attgaagaag gagctacaca gatttttatt cttccatttt ctgaattatg atatttttcg
    17761 aatacccttc tcaaatcttt ttcaaccgtt tccacatctt ctataagctc tttgtgaata
    17821 cctaaatcta tacaacctct acctacaacc cctcttatac ctaaatcttt atatgccttt
    17881 actattccat ctgtaagtcc tggtctattg tgagtatgca tataatcaac cattgtagtt
    17941 ataccacttt ttaaaccttc catacatcca agcattgcag catcataagt atcttgttct
    18001 gttaagaatt ttgctgatgg aaacatcatt gtatttaacc actcatgaag tgccatatca
    18061 tcacctaaac cttttaataa cacttggaac aggtgattat gagtatttat aaagccaggg
    18121 aatattactt ttccattagc atctataact tttttagcat caagatactt aggctcaata
    18181 tcaactgtat ttcctatgtc caagattctg tcaccttgaa tagctattgc tccatcaaat
    18241 ataacatctc tgtctttatt aactgtaaca attattgcat tttttattag tatatcaatc
    18301 atcttaaatc cccctttgaa tactttcatt acaacatttt accatatggt tttctcatat
    18361 tatatctaat acatttatcc aatattttcc tcaagatttg aagtaacgtt ttcagcactt
    18421 ttacgtgact ttaatttaaa taatgtttga agtaatatag ctattattgc tccagaaact
    18481 atacttgacc ctccaaggac agttccaatt gtttctggga attggtctaa aacttgtgga
    18541 gcagttgtaa gacccatact tgttgctata gatacacctg ctataagatt gttttcttca
    18601 gtgaatccat ccatactcat aatagagaaa ccagacattg ctattgatga gaaaactact
    18661 aaagttgctc caccaacaac acaagatggt atagttgtca ttattgcacc caatgctgga
    18721 gatatccctg ctagtaataa tacaagtgcc cctagtgcta ttacaaatct acttgtaact
    18781 ttgttcatag atacaattac tgtattttgg ctaaatactc cagttggaag agctgcaaat
    18841 agtgatgaaa ttatagaagt tatagaattt cctaaaacaa ctgatgctaa ttcttcgtca
    18901 gttacctctc tatccattgc tcctactgta gctattgtac aggcacccat catatcagct
    18961 atacctatta tgtaaataat agtaaacatt actattaact ctggtctaaa ttctaatcca
    19021 aaagctgctg gtgttggtaa agatactata gctgctcctt gaacggctga gaaatcaacc
    19081 aatcctaaaa ctaaagatat agcataacct actaatattc ctattaatat tgaagaattt
    19141 tttactaaac ctttaccgta tttatttatc attattatta caaaagcaac tataactcct
    19201 actgtgaagt ttattgggct tccaaatgtt tgtgtgcctt ctcctccagc taaattcttt
    19261 atagctgttg cgaatagtcc aagtcccata catgctatta tagttccaga aactattgga
    19321 gtgaatatat gtctaatttt cttaactcca aatcctacaa agaatacgat taccccagca
    19381 gcaatctgtg ctccaaatag agctgctaaa ccatctttag ctgctactga aagacaagct
    19441 cctaaaaata cataactcat tcccatcatt acaggtaatc ttgaacctaa cttaaatcct
    19501 ttaaataaag gtattggata taactgtaaa aacgttgcta aacctgctgc taacatagaa
    19561 ccctgaatca acattgttgc ttgcccctgg ttaagtccta ccacgtttgc tattaatatc
    19621 gcaggtacca tatttcctac tatcattgct aacagatgtt gtaaggccaa aggtattgat
    19681 tcctttagtg gtggtatccc atcgacatca tattttgaag tattacgtgt tttcataaaa
    19741 taaatcctcc catacataaa tatcttgact aaatattttt ataattattt tctataaata
    19801 tttttatgac catgaattta ttgctcaacc gataaacaca gctaaactga gctattttta
    19861 taaaaaatta acaaatttct aaatattccc tccccgattt tcaacttaaa tgaacattaa
    19921 ttgcctggta tatatttatt tgttaaattt tttattaaaa tgaatagcta tcaaaaataa
    19981 tctaacaatc tcatactaaa taatttaaac aattctttta aattattata tcttaattag
    20041 tctcttccat aacagctctt tctttagcct catcctctag acttttattt ggtattatta
    20101 gatttaaaac gaatgcaacc aatgttgcaa ttactattgg agattctcca aatactaact
    20161 ttacaaatga aggaaatgcc tcttgggctg ctggtactga tgttattccc atgcctaagg
    20221 caattgccaa accaacaatg gtaatatttc ttgatgacaa ctcttctgta ataattaact
    20281 ttatacctgt cattgttatc atactaaata tagatattgt agctcctcct aaaacagaat
    20341 atggtatagt agtcattagt gcaccaaatt taggcacaaa ccccgacaat aacataaata
    20401 taccagctat tccaagcaca aatctactta ttactttgtt catagctacc atccccacgt
    20461 tttggctata tgatgatggt ggtaaacctc caaacaaagc tcctatcata gtacaaattc
    20521 cacttcctat tactccactt gataattcct tatctgtgat attcctatcc atgcctccca
    20581 ttgtagtagc tgataaatct ccaattgctt gaacagcatt gattatactt acaattacta
    20641 ctgagattat tacaggcata tcaaattcca tcccaaattc aagcggttta ggtatcgcaa
    20701 accaagttgc ctctctaata gggtcaaaat ttattatacc cagaactaaa gatacacaat
    20761 atccaactat aattcccaaa aatattgctg ccaattttgc atatcctttt gcaaattgac
    20821 taagacctat aactaccact aatgttatag ccgctactgc ccagttttgc atagaggcat
    20881 aagtaggact atttacacca cctgccatat aatttattgc tataggatac aaagataatc
    20941 caattgtaaa aaccactgtt cctgcaacta tattcggaaa aaattttctt atctttttta
    21001 tgaatatccc tattaatact gtagccactc cccctattaa ttgtgcaccc aaaatgcttg
    21061 ctaatccata atttcctccg actgctacaa gtgttggtac ataagtaaaa ccaactccaa
    21121 aaattatagg caatcttgaa ccaatatttc ctattggata tagttgtata agtgttgata
    21181 taccagctat tattaatgca tattgcacta aaagtgttat ttgtgtaggg ctaacaccca
    21241 cagctcctcc tacgactata ggtactgtaa cagttccaac tatcattgct agtatatgtt
    21301 gcattgatag tggcaaacac tttaaaattg gtggccttcc atcaatatca aacatagaaa
    21361 cattcttttg atatttactc aaggttatca ctcccaacta ttttttaatg acagttatta
    21421 ttatctcaca caacatcctt tcttttttta ttttgtcatc tcaacttaat atgaagcaat
    21481 tataatgcca attttttaac ataattgttt tacatatata atttttatta tatttgttag
    21541 tatttgataa tttattaaag catttatttt tttaaattct tttaaatttt ttttattttt
    21601 ggataataaa atgctattac acaaaaaaac acttagaaaa tagttttcca agtgtttttt
    21661 atcaatttta taatttcaaa ttttagaatt gagaataaaa agcctaatta ttatacatct
    21721 atttttctgt acaatgtagc tagtgatatt ccaagttttt tggcagctag ttttttagac
    21781 ttagtatcat ttccgtaaaa ctttaacact ttctttatat aggctctttc aaactcttca
    21841 agtgtaagta tgtcttgttc catacttatc tcatagtcac tttcctcaag taactttata
    21901 tttatattat ttaacttaaa atattcaagt atctttctat ttattattcc tttatgtatt
    21961 attccatctt cctcaagaag gtttaatgcc agttctatag tattttccaa ctctctaata
    22021 tttcctggcc aaggatactt aagcaacatt tgttccacat ctggctctat atggtacacg
    22081 tttttcttca tctttctcga atatttgtct ataaaattgt ttattaatat ttgtatatcg
    22141 tctcctcttt ctctaagtgg aggcacttct aaaggtatta cattcaatct atagtataag
    22201 tcttctctaa atttattttc cttaaccaac tcaaataaat ctctattagt agctgatatg
    22261 attctaacat ctatatcaat tagttcattt gaacctattc tcgtaaactt cttttcctgt
    22321 aataccctca ataactttga ttgtagttga attggcatat ctcctatctc atctaaaaat
    22381 acaacccctc cattggccaa ttcaaactta ccaattttac cttttgaatc tgctccacta
    22441 aaagctcctt ttgtatatcc aaaaagttca ctttctagta aaggttctgg tatagcagca
    22501 cagtttatag caacgaatgt tttattccct ctgtttcctg cgagatgaat ggctcttgct
    22561 attaattctt ttccagtgcc actttctcca gttatagcta cagtagaatc actatttcca
    22621 attttacaaa ttctattctt aagagcacgc attacatcag actctccaat tatggagtct
    22681 actgttgttg ttccccaaat attatatact tccattccac catacattac tgaatcactg
    22741 tgtttgtcca taaaatatca actccaaata agtattctat ataataatca ttcacattat
    22801 ctatttaatt atatcataac agaaaagctt agttataatt taataatatt attttaaaac
    22861 aaaacttaat aaaaagtata tctacttagt tttaatttga tagatatact ttcttcattt
    22921 tacaaaaatc ctctatt
//
LOCUS       DAYATN010000053        22218 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.053, whole genome shotgun sequence.
ACCESSION   DAYATN010000053 DAYATN010000000
VERSION     DAYATN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22218
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.053"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            188..364
                     /locus_tag="ACGI8A_001925"
     CDS             188..364
                     /locus_tag="ACGI8A_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179922.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            538..1668
                     /locus_tag="ACGI8A_001926"
     CDS             538..1668
                     /locus_tag="ACGI8A_001926"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFH0179923.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            1909..2259
                     /gene="eutS"
                     /locus_tag="ACGI8A_001927"
     CDS             1909..2259
                     /gene="eutS"
                     /locus_tag="ACGI8A_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFH0179924.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            2267..2716
                     /locus_tag="ACGI8A_001928"
     CDS             2267..2716
                     /locus_tag="ACGI8A_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFH0179925.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            2818..3393
                     /locus_tag="ACGI8A_001929"
     CDS             2818..3393
                     /locus_tag="ACGI8A_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0179926.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3386..4795
                     /locus_tag="ACGI8A_001930"
     CDS             3386..4795
                     /locus_tag="ACGI8A_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HFH0179927.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            4979..6412
                     /gene="eutA"
                     /locus_tag="ACGI8A_001931"
     CDS             4979..6412
                     /gene="eutA"
                     /locus_tag="ACGI8A_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFH0179928.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6430..7794
                     /locus_tag="ACGI8A_001932"
     CDS             6430..7794
                     /locus_tag="ACGI8A_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HFH0179929.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            7807..8688
                     /gene="eutC"
                     /locus_tag="ACGI8A_001933"
     CDS             7807..8688
                     /gene="eutC"
                     /locus_tag="ACGI8A_001933"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HFH0179930.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8708..9361
                     /gene="eutL"
                     /locus_tag="ACGI8A_001934"
     CDS             8708..9361
                     /gene="eutL"
                     /locus_tag="ACGI8A_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFH0179931.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9372..10073
                     /locus_tag="ACGI8A_001935"
     CDS             9372..10073
                     /locus_tag="ACGI8A_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFH0179932.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            10076..11545
                     /locus_tag="ACGI8A_001936"
     CDS             10076..11545
                     /locus_tag="ACGI8A_001936"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HFH0179933.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11641..11928
                     /gene="eutM"
                     /locus_tag="ACGI8A_001937"
     CDS             11641..11928
                     /gene="eutM"
                     /locus_tag="ACGI8A_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFH0179934.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            12055..12816
                     /locus_tag="ACGI8A_001938"
     CDS             12055..12816
                     /locus_tag="ACGI8A_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HFH0179935.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12829..13458
                     /gene="eutD"
                     /locus_tag="ACGI8A_001939"
     CDS             12829..13458
                     /gene="eutD"
                     /locus_tag="ACGI8A_001939"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization phosphate
                     acetyltransferase EutD"
                     /protein_id="HFH0179936.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13500..14225
                     /locus_tag="ACGI8A_001940"
     CDS             13500..14225
                     /locus_tag="ACGI8A_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HFH0179937.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            14238..14510
                     /locus_tag="ACGI8A_001941"
     CDS             14238..14510
                     /locus_tag="ACGI8A_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFH0179938.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14503..15054
                     /locus_tag="ACGI8A_001942"
     CDS             14503..15054
                     /locus_tag="ACGI8A_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFH0179939.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            15066..16157
                     /gene="eutH"
                     /locus_tag="ACGI8A_001943"
     CDS             15066..16157
                     /gene="eutH"
                     /locus_tag="ACGI8A_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFH0179940.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            16159..16632
                     /locus_tag="ACGI8A_001944"
     CDS             16159..16632
                     /locus_tag="ACGI8A_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFH0179941.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(16752..16847)
                     /locus_tag="ACGI8A_001945"
     CDS             complement(16752..16847)
                     /locus_tag="ACGI8A_001945"
                     /inference="COORDINATES: protein motif:HMM:NF012392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0179942.1"
                     /translation="MGYPNVEHFIRLFNKKYNMSPSKYRKNTSKI"
     gene            17148..17669
                     /locus_tag="ACGI8A_001946"
     CDS             17148..17669
                     /locus_tag="ACGI8A_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0179943.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            18048..19223
                     /locus_tag="ACGI8A_001947"
     CDS             18048..19223
                     /locus_tag="ACGI8A_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0179944.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            19258..20796
                     /locus_tag="ACGI8A_001948"
     CDS             19258..20796
                     /locus_tag="ACGI8A_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFH0179945.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            20828..22030
                     /locus_tag="ACGI8A_001949"
     CDS             20828..22030
                     /locus_tag="ACGI8A_001949"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFH0179946.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
BASE COUNT         9003 a         2503 c         4133 g         6579 t
ORIGIN      
        1 ttctttacta ttcaagttct aaatcaataa taaattttga tatttaattg tatcaggaca
       61 aatctctaat ttcatagatt attaatataa aatcataggt tagtgtgaaa aagttgaaat
      121 ttaaatattt taaaagataa tttttttgaa ctaataggta taacaaaaat ttataggggg
      181 attgcatatg gaaatcaatg ttatagaaat tttccctaaa gataaagcta aacttaataa
      241 aatagaaatg gataaagcta gttggtttgt aaatataata agtaaaaaat atcctaaaga
      301 agctttaaat gaagcattta gtactttaga aaaagaatta aatataagta aagctaatac
      361 ataagctttg attattgatt ttatatacta ttggttttat acgctatgac ttgacattat
      421 attaatcaaa tagtatattt ttaaatatag cacaatgaag tgttaaaaga gcaatggagc
      481 tccatagata aagtctatgg agcttttttg cgtatttaac ttaaggagga taggactttg
      541 aataactttg agattaaaac tagaataaaa tttggagaag gctcactaaa agctttgcaa
      601 gaattaaaaa acaaaaatgt actaataata acagaccctt ttatggtgaa atcaaaaaca
      661 atagataaaa taacatctaa tttaataaac tcaacatatc aagtttttga agaaatagta
      721 ccagacccac caatagaagt agtagtagca ggaatagaag catttaaatc tataaatcca
      781 gacgtaataa tagcattagg tggtggttct gcaatagatg cagcaaaatc tataatggac
      841 ttttctaaaa aaatattaaa aattaacgaa gtagagttta tagcaatacc aacaacaagt
      901 ggaacaggtt cagaagtaac atcatttgct gtaataacag atagtcaaaa aaaatgtaag
      961 tatccattag tgagtgatga tttacttcca gatatggcaa tactagaccc agaacttgta
     1021 aaaactgtac caaattttat aacagcagat acagggatgg atgtattgac acatgctata
     1081 gaagcttacg tatctacaaa ttctaatgac ttttcagatg cactagctga aaaagctata
     1141 aagttagtat ttaaatactt aataaaagca tataaagatg gaaatgacat agaagctaga
     1201 gaaaaaatgc acaatgcatc ttgtttagca ggtatggcat ttaaccaagc atcacttggc
     1261 ataaatcatt ctattgcaca tgtattaggt ggaaaattcc atataccaca tggaagaaca
     1321 aactctatat tacttcctca tgtaatagaa tataattcat caatagtagg atatactaac
     1381 acaacttact ctgctacagc taaaaaatat gcacaaatag cagaattagt aggacttggg
     1441 agtgctaata taagaatatc tgttagaaat ttagtaaatg aaataaagaa actacaaaaa
     1501 gaaatgaata tgccaactaa attgacacaa tgcaaattaa gtttagaaga tataactaat
     1561 gaagaaagag aaatagcaaa acttgctata aaagatactt gtacattaac taatcctaag
     1621 gtaccaactg aagatgatat aataaacata ttaaagaaca ttaaataact acattttatt
     1681 acatgtaaat ataatattat acaaataggg aaaatgtttt taaaaatata aattatatat
     1741 tgacaaatat aaaacataaa actataatga aagtatcaca aaggcgtgat actttagtag
     1801 aaagcaatgg agcttcttag gaatattaat attattctta tggagctcct tttattttaa
     1861 tcaaatagct ttttaataaa ctatattcaa agaaagcagg gaataaattt gactgaagaa
     1921 tctaagcaaa gagttattca agaatatgtt cctggaaaac aggttacatt agcacatatc
     1981 atagctaacc ctaatgaaga tatatataag aagttaggat tagtacttga taaaaaagat
     2041 gcaataggaa tacttacaat aacaccaagt gaagcgtcaa taatagcagc agatgttgct
     2101 acaaaggctt caaatgtatc tttaggtttt atagatagat ttagtggttc agtagtaata
     2161 tcaggtgatg taagttctgt agaatcagcg ttaaatgatg ttttagaagt cttaggaaat
     2221 atgttaaact tctcatctac aaaaatcaca aggacataat aatacaatga agaatataat
     2281 atttatggga aagacaggta gtggtaaaac taccttatgt caaaaactag accagttaga
     2341 attaaagtat aaaaaaactc aatcagtgga actttataat aatgcaatag acactcctgg
     2401 tgagtatatg gaaaatagaa acctatataa tgccttaata acaacatcag cagatgctaa
     2461 aattatagca atagtgtatg atgccacaca agaagaaaat tatatagcac caggttttgc
     2521 cagtatgttt tgcaaagacg taataggaat tataactaaa ataaataaag ctactgaaaa
     2581 cgaagtcaat atagcttatg aaagactatc actagcagga gcaagtcaaa tctttaaaat
     2641 agacacagtt gataatattg gacttgaaaa attatttaaa tatctagaaa aatataaaaa
     2701 tatagatgaa gtttaagggg aaatattttt ttattcaaaa atattattaa agtatgataa
     2761 aataaaaaaa tgtataaact attttaagaa gatttaatct ttaagcgagg taggaacatg
     2821 gggaaaaaaa tactattggt tgatgatgaa ccactgttaa ggatggatac aaaagatata
     2881 ttagaaagtc atggctatga aataataggc gaagcaagtg atggatttga agctgttgaa
     2941 atgtgtaaaa aacacaaacc tgatttagtt ataatggata tagagatgcc aatacttgat
     3001 ggtataaagg caggcaaaat aatatgtaaa gaaaatatag ctggtggagt acttttatta
     3061 acttcatttg aagataaaga atacgtagaa aaggctaaaa gtataggtgc ttatggatat
     3121 ttagtaaaaa cagcaagtga aaaatcactt atcccaacca tagaaatgtg tttaaataag
     3181 gtaaatgaat ttgaaaaaat gaaaaaagat ttagaaaaag taaattctaa attgaatgaa
     3241 agaaaattag tagaaagagc aaaaggtata cttataagag agtttaagat ttctgaggat
     3301 gaagcataca ctagaataag aaaattaagt atggataaaa gaactacaat gatggaaatt
     3361 gccaaaatga ttataatagg atacgatgac taatacgata aaaaaaatat caactgcaca
     3421 aacatactta aataataacg aaattgaaaa aattatagaa gtatcaaaat ccttagattt
     3481 aatggcttct ttttatgaag ctgatgtttt tatagatgta ctaagtaaat ttgatgatga
     3541 agcaattgtt gtggcacatg gtagacctaa agaaaagtca atgtactgtg aaaatgttat
     3601 aggtaaaaag gctttaattg aaaatgaacc aggagttata aagtgcttaa aaacaggaga
     3661 aagagtaaga gatattaaag ctttaacaca ggaatataaa ctggtcaaac aaaatattca
     3721 gccaataaca ctagatgaca aagttttagc agtactaata gttgaaaaag atataagtag
     3781 tgaagttaag aatgagttta atgctagtga agacgaagaa aataaaaatt tacatcagtt
     3841 tataaaactt attggaaata atgacttttt agttgataat ttaaattcag ccatattaat
     3901 atttgataaa aatggaaaat taaaaattaa aaatcgtatt gcaactgaga tatacaaaaa
     3961 tcttgggttt ggatatgata tacaagatat gcattacaat gatttagata tagaaaccaa
     4021 cacctttgaa aatatattaa tggatgaaaa ttatgatgag acaaatgaag tttcaatagg
     4081 taatatgttt tttaaaataa agaccatata catcaaagat gatgaattta aggtttgcaa
     4141 aatagtacaa gatataagtg atttaaagag aaaagaagaa gaattagttt tgaaaacagt
     4201 ggctataaga gaagctcatc atagagtaaa aaataatctt catacagtaa tatctataat
     4261 aaaaaaacaa agtagattat ctcaaaatga tgaagtaaag cagtgtttag ataacataat
     4321 aaatagggta tttgcaatac tttcgactca ttatttacta tctaaagaag ttgataataa
     4381 tatatctata cgagaaggta tcaatttatt agtgtcaaat attcaaggtg gctatttatt
     4441 tagtaaggat atagatatac gtataactgg tgaagatttt aaaattagtg gagaaaaagc
     4501 aacagcaata cttttggtta taaatgaaat actacaaaat tgttatgacc atgcattttc
     4561 ggatagagaa catggtacta tacaaatatt aataaataaa gataacgact atagaaatat
     4621 tgctattata gatgatggaa ttggatttga tagtaataat ataaataaaa atagcttagg
     4681 gacatatata atagatagtt atataacaca gatgttaaaa ggaaatatag agagaaaaag
     4741 tagtgagaat ggtacagaag tattaattac tatacctaca caaattaata tttaaataat
     4801 aactcatcta caaagtagta gttttaattg agcaaagagg cttaaggtta ttaatagatt
     4861 aagttaattg aatgtattta taaaaattat aagtatatat tcaatttaac aggtatttat
     4921 tataacttta agcctctttg cttgtttaag acatacttaa attttaggag gtgagaaaat
     4981 gagagaagag ttattaagtg ttggtattga cattggaaca agtactactc agttaatttt
     5041 ttctaaatta atagtagaaa atactgaatc aaatttttcg gtacctagaa taagtatagt
     5101 agacaaagaa attatttata aaagcaaagt ttatttcacc cctcttattt caaataatga
     5161 aataaatggt caacaaataa aagatatagt tgaaaatgaa tataaaaaag caaatatatc
     5221 taaagaagat atacatacag gagcagtaat aataactggt gaaacagcta gaaaagaaaa
     5281 tgcaaatgat gtacttcata ctttgagtgg atttgctggg gacttcgttg ttgcaacagc
     5341 aggacctgat ttagaaagta taatatcagg aaaaggagct ggagctcata tttattcaaa
     5401 agaacattca actacaattg taaatataga cataggtggg ggaactagta acatagcctc
     5461 atttaaaagg ggagaagtaa ccgatactgg gtgtttggat ataggtggaa ggcttgtaaa
     5521 gatagataaa ataagtaaaa aaataactta tatatcacct aaaatagata aaatcataaa
     5581 agataataat ttaaatcttc aagtaggcac aatggctact gtagaaaata caaagccaat
     5641 agtggatata atggttgact tattaatgga aagcatagga ttaaagaaac agacaccttt
     5701 gtttgatttt ataataacca ataaatcaat aagtatagat gaaaatatta aaaatgtttc
     5761 attctctggt ggagtagctg attatattta ctatgatgga gaaataaaag attattttga
     5821 atatggtgac ttgggaattt tacttgggca agcaataaaa aattgtgaat accttaaaaa
     5881 agtaaatgta gttaaatcta tggaaacaat aagagccaca gtggttggag caggttctca
     5941 tactactgaa gtaagtggaa gtacaataac ttacactagc gaaaagtttc cacttaagaa
     6001 cttgccagtt ttaaaattat caattgatga agaaacaggc acttcaaaac aaatcagtga
     6061 agctataaaa aataaactta attggtttaa actagaaaat gatttacaga aagttgctat
     6121 agccatgata ggaaagaaaa atccaacctt tttagatata caagagtatg ctaaagggtt
     6181 tgtagatgga atgcaacaaa tcatagaaaa tgatttacct tttatagtaa ttgttgaaaa
     6241 tgatatggca aaagtgttag gtcaagcaat atatgcatta ttaaattatg aaaaagaaat
     6301 tgtttgtata gacagtgtca aggttgaaaa tggagactat atcgacattg gaaaacctat
     6361 agttgaaggt aaagttttac ctgtggtaat aaaaacatta gtttttaatt aatagaagga
     6421 ggtaagtaaa tgattttaaa tacaaaatta tttggacgta cttatcagtt taaaggctta
     6481 tatgatgtaa tggctaaagc taatgaagaa aaatcaggag ataaattagc aggtcttgca
     6541 gcagaatcag ctgaagaaag aatagcagca aaagttgttt tatcacacat aaaattggag
     6601 gatataagaa ataatcctgc agtggcatat gaagaagatg aagtaacaag aataatacaa
     6661 gatgctgtaa atgaaaaagt ttatgaagaa ataaaaaatt ggacagtctc tgaacttaga
     6721 gaatggctat tggattcaaa tactacaagt cttgatataa gaagaatatc aagaggatta
     6781 acttctgaaa tgatagcagc agttgcaaaa ttaatgtcaa atatggactt agtttatgca
     6841 gctaaaaaga taaaggttac tgctcactgt aatacaacta taggagaaca aggaacatta
     6901 tcagcaagac ttcaacctaa tcatccaaca gacgacccag atggaatact tgcatcactt
     6961 cttgaaggtt taacttttgg tataggagat gcagtacttg gattaaatcc agttgatgac
     7021 tctgttgaga gtgttactaa agtattaaaa agatttgaag aagtaaaaca aaaatatcaa
     7081 ataccaactc aaacatgtgt attggcacac gttactacac aaatggaagc aataagaaat
     7141 ggtgctcctg cagatttaat attccaatct atagcaggtt ctgaaaaagg aaatgaagca
     7201 tttggattta atggcgacac tatagaagaa gctagacagt tagccttaaa acacggtaca
     7261 gctacaggac ctaacgttat gtattttgaa actggacaag gttcagaatt atcttcagaa
     7321 gcacatcatg gaatagacca agttactatg gaagctagat gctatggatt tgctaaaaag
     7381 tatgaaccat tcttagtaaa tacagttgtt gggtttatag gacctgagta cttatatgat
     7441 gctagagaag ttgtaagagc aggacttgaa gaccacttta tgggtaaact acatggctta
     7501 ccaatgggtg ttgatgtttg ttacactaac catatgaagg ctgaccaaaa tgatatggaa
     7561 aacttagcca tactattaac aacagcagga tgtacgtatt ttatgggaat acctcatgga
     7621 gatgatgtta tgcttaacta tcaaacaaca ggataccatg aaacagcatc acttagagaa
     7681 atgtttggac taacagcaat aaaagaattt gatgcttgga tggaaaagat gggattctct
     7741 aaagatggta agttaacaga gttggcggga gacgcatctg tattattagg ataggaggta
     7801 tttaatatga atgagaagga tttaaaagca ttggtagaac aattagttgg acaaatggtt
     7861 ggagaattag atactaatgt tgtgtctgag actgttaaaa aggcaactga agttgtagta
     7921 gacaacaatg cttgtataga tgatataaca gaagttgata taagaaagca attactagta
     7981 aaaaatccta aggatgcaga agcttattta gatatgaaag caaaaacacc tgcaagatta
     8041 ggtataggaa gagctggtac tagatataaa acagaaacag ttttaagatt tagagcagac
     8101 catgctgctg cacaagatgc tgtattctct tatgtagatg aagaatttat aaaagaaaat
     8161 aatatgtttg cagtggaaac tttatgtaaa gataaagatg aatacttaac aagaccagat
     8221 ttgggaagaa aattttctcc agaaactata aataatataa aatctaaatt tggtacaaat
     8281 caaaaggttt taatattagt tggagatggg cttagctcag ctgcaataga ggcaaattta
     8341 aaagactgtg tgccagctat aaaacagggg ctaaaaatgt atggtataga ttcaagtgaa
     8401 attttatttg ttaaacattg tagagttggt gctatggacc acttaggtga agaattaggt
     8461 tgtgaagtta tatgtatgtt ggttggagaa agaccaggat tagttactgc tgaatcaatg
     8521 tcagcatata tagcttataa gccatatata ggaatggcag aagcaaaaag aacagttata
     8581 tcaaatattc acaaaggtgg aacaactgca gttgaagcag gtgctcatat agctgaactt
     8641 ataaaaacta tgttagataa aaaagcttca ggaatagacc ttaaataaag tcgagggagt
     8701 gtagaatatg aaaaatgatg taattcgtcc taatatacta ggaattaaaa taatttcaaa
     8761 tataagtcca gaaatggcac aaaaactaga attaaaatca catcacaaaa gcttaggttt
     8821 aataactgca gactgtgatg atgttacgta tacagctctt gacgaagcaa ctaaagcttc
     8881 agaagttgat gttgtatatg caaaatcaat gtatgcaggt gctgcaaatg cttcaacaaa
     8941 acttgctggt gaagtattag gaataatagc aggtccaagc ccagcagaag taagaagtgg
     9001 tctaaatgca gtagttgatt ttctagagta tggagcaact tttataagtg caaatgatga
     9061 tgattcaata gcatactatg ctcactgcgt atcaagaaca ggtacatacc tttcagaggt
     9121 agcaggaata agagaaggtg aagcattagc ttatttagta gcaccaccac ttgaagcaat
     9181 gtatgcacta gatgcagcaa tgaaagctgc agatgttaag atgtgtgaat tatttgcacc
     9241 tccaactgaa acaaactttg gtggagcatt acttacaggt tctcaatcgg cttgtaaagc
     9301 agcatgtgat gcatttgcag aagcagtaaa atcagttgca gacaatccaa caggatttta
     9361 aggatagtta gatggatgct ataggcttaa tagaagtaat aggatatgtt gctgcaatag
     9421 aagcttctga tgcttgttta aaatctgcaa atgttaatat tgtaagagtt gataaggttg
     9481 gagcaggtat tgttacttta acaatatgtg gagatgtcgg tgctgtaaaa gctgctttag
     9541 aagcaggtga aatagcagct tccagagttg gtacattaag aacatcacat attataccga
     9601 gaatccataa tgaagtaaca aatgctttat tcaaaactaa agaacctaaa gtctgtgaag
     9661 tttcagaaga taaagaaaaa aactttgaga ttacattgga aatagctgat aatgtatcga
     9721 aaaatttatc agaaaaagaa aatgtaattt tagatgtcaa tgaaaacagt ggagatactg
     9781 aaaatttata tagtgatata gataaaacaa acaaaaaaat tgaaacagtt gatgatagtt
     9841 taacagaagc taatgaggaa aaaatagaaa acactaatga taaagaagta ttggaacaag
     9901 ttaaaataga aaattccgat tttgatagag atgacctatc aaagaacagt gtaaaaaaac
     9961 ttaaagctat ggctaagaaa ttagattctt caataactta taaagactta aatgttctta
    10021 aaaaagaaga attaataaac ttagttaaaa agcttagtag aagggataga tagttatgac
    10081 acttttagat aaagatttag tatctataca agaagtaaga actcttgtaa gaagatctaa
    10141 agtggctcaa gagaaacttt ctaaaatgag tcaagagcaa atagataaaa tagtaaaagc
    10201 tgttgtagat gcagcattta ataatgctac taaattagct aaaatggcaa atgaagaaac
    10261 tggatttgga aaatgggaag ataaagtaat taaaaatact tttgcatcta tgggagttta
    10321 tgaaacttta aagaacacaa aaactattgg aataattggt gaagataaag aagccaatac
    10381 gatagatata gctacaccag ttggagttgt tgcaggtctt ataccttcaa caaacccaac
    10441 atcaacagta atatacaaaa ctataatatc tctaaaagca ggaaatacta tagttttctc
    10501 tccacatcca aatgcaaaaa actgcatact tgaaacagtt aatataataa gtgatgctgc
    10561 tataaaggct ggagctccag aggattcaat attatgtata actaatccaa gtatagttgg
    10621 aaccaatgaa ttgatgaaac ataaagatac aaacttaata ctagcaactg gaggagaagc
    10681 aatggttaga gctgcttact cttcaggaac accagctatt ggagttggac caggaaatgg
    10741 accagcattt atagataaat cagcaaatgt taaattagca atcaaaagaa taatagattc
    10801 taaaactttt gataatggtg ttatatgtgc atcagaacaa tctatagtaa tagaaaaatc
    10861 tatggaagaa actgttttaa aagagcttaa aaatcaaggt gcatatgttt taaatgagga
    10921 ggaaagtgaa agattagcaa aattcatatt aagagctaat ggtactatga atccacaaat
    10981 agttggtaaa agtgttgagg taatagcaaa tttagcagac ttaacaaatg tacctaaaaa
    11041 tacaaaagtt ctagttgcaa gagaaaataa agttggtcac aatgtagcct attcaagaga
    11101 aaaacttaca ccaatactag cgttctatgt tgaagaaaat gtagaagcta ttatgaacag
    11161 atgcagagaa atattgttaa atgaaggtgc aggtcataca ttctcaatgc atgtaaatga
    11221 tgaagaattg gtaaaaagat ttgcccttaa tatgccagta tcaagaatac ttgtgaatac
    11281 tccttcagca cttggaggaa taggtgcatc aacaaacctt gtaccagcac taacacttgg
    11341 ttgtggtgca ataggtggaa gttcaacttc aaacaatata ggaccacttg atttaataaa
    11401 tgttaaaaga gtagcttatg gaatcaaaga aatagaagaa cttggaagag ataaatctca
    11461 agttgaaaaa tcaatgtttg actcaatgga taaagaagaa ttaataaatc ttcttgttca
    11521 aaaactagtc aaagaactag cataactaaa ataaacaatg aaacttatgg ctatcgctat
    11581 aaatttctaa aaatataaac tataaaaaac tataaaatta aatttttgga ggataagatt
    11641 atggcaagtg caaacgcatt aggaatgata gaaactaaag gattagtagg agcaatagaa
    11701 gcagcagatg caatggttaa agctgcaaat gtacaattag taggaaaaga gcaagtaggt
    11761 ggaggattag ttactgtaat ggtaagagga gatgttggag ctgttaaagc tgctacagat
    11821 gcaggagctg ccgctgccga gagagttgga gaactaatat cagttcatgt tataccaaga
    11881 ccacactttg aagtagatgc tatattacca aaggtatcag ctgagtaata taagaataat
    11941 tataaaattt aaattttgat tgaaaaagag gctatctcaa aaatgagtct tactaataaa
    12001 ctcaatgtat tttgaggtag tcttttttat acatctaata ggagatgtga taaaatgggt
    12061 gttttaacag aaaacgaagt aaaaagaaaa attggaaaaa acaaaaataa tataactgaa
    12121 ttttatatag aaaaaggtca aataataact ccttctgcaa aatcttattt agctgagaga
    12181 agtattgagc ttaaatatgt tgataaactt tcagatgtaa acaatgaacc taaagaagtt
    12241 gtaaaagaag tagttaaaga gaaaaaatat agatatacta ctgtatttgg cgctaaattg
    12301 gatgaaaaac cagaacatat gactcaatta aatggaaatc ttttagtttt taaagaccat
    12361 aaaagaataa tattgagagg aaaaatagat tctcttgaat cagaaatact aaaggtacag
    12421 atactttgtc agaaactaga tatgccaaag cttgttaagg aattgcaaga agtattagac
    12481 tttgtaagaa atataatgag atgtgaagtt ttagatgaaa agctagggga ctttaaactt
    12541 ttaggattaa ctcctcaaga acttagagaa caatctcact atcctaaaaa atactttggt
    12601 ataggacatg agttcccaag ttatgaaatg ggagaagtag tagtagctat aaatagtata
    12661 agaagtaata cacgagaggt tgaacttgca gcctatgaag catttaaggg tgagtatgga
    12721 aatgttcaca gagaagattt aataaaggca cttaatagat tatcgtcagt attttggata
    12781 atgatatata aggttagaac taataagtat aagtagttgg aggacataat ggagaatcta
    12841 ttaaatgaaa tagtggaaga agtaataact agagttaaaa aagaagcatt tatagaagtt
    12901 gaagcatcag gaagacacgt tcacttaagt agagaagatg tcgataaatt atttggagaa
    12961 ggatatactc ttacaaaact aaaagaccta tctcaaccag gtcaatatgc atgtaaggaa
    13021 agagttacaa taactggtcc taaaggaagt ataaaaaatg tagttgtact tggaccatgt
    13081 agaaatgaaa ctcaggtaga aatatcttta acagatggtt caactcttgg actaaaagct
    13141 cctataaaac aaagtggaga cttagaaggt actttaagta ttaaaatatc aacacaatat
    13201 ggtgaagttg aacttgataa ggggttaatg gttgctaaaa gacatataca catgactcct
    13261 aaagatgctg aaaaattcaa tgtatgtgat aaagagatag ttgaagctaa ggtgatgggt
    13321 caaagacctt taacatttga tgatgtagta ataagagtga gtgacagttt taaaacttat
    13381 atgcatatag actatgatga agccaatgca tgtggttatt caaaaggaac tgttgctaaa
    13441 ataataaaga aagcgtaaat ttaacctact atataaatta cctctaggga ggatttaata
    13501 tgaattatga taaccttgta gatttaataa cggaagaaat ctataaaaaa ataaatagta
    13561 atgaaataaa aatatctaat aaacctaaag ctgtgattgt atttgaacaa gataacaata
    13621 aatttaactt attaaaagat gaatttgaaa tagtagaatt tgataaaagt ataagggaat
    13681 gtgaaatagt gatagtttca agactttgta tgagggggct atcgaatata gcacttggaa
    13741 attcaacaag tgatgaagaa agatttatac taaaaatgat tatgaagggc aaagaagtat
    13801 atgtcctaga tgaaggaatt gaatacaaaa aatataagga tactgcacca aaagcattat
    13861 ataagaaatt tatgtcattt gaagatgaaa tatgtaaatt tggtgtagaa attataaaag
    13921 acttaaatgt tataactaaa aataagttaa atataaagaa agaactagaa agtactagag
    13981 gttctaaaaa agaagataca aaagttttag ataaagatgt tttaaattta accagtgtag
    14041 aaagtgtaaa aatagataat gaatttagtt tagatttaag gaataaaaaa cttatatcgg
    14101 aagctgactt aagaaaacca actataaatg gagttaaaaa catattagtt aataaaaaaa
    14161 gtataataac accattagct gtagatttta tgagaataca ccatctcaaa ctaaagaaag
    14221 tgtaaggagg taattttatg gatataggaa aagttgtagg taatgtttgg gctacaagaa
    14281 aagatgaaaa attatgtggc caaaaacttt tagttgtaaa aattttaaaa actaaagaca
    14341 catacaagga aggcttcatg gttgcagccg ataatgcagg agcaggaaat ggagacttag
    14401 ttcttatatc caaaggttcc tcagctagag agtcgattga aaaatctcat gttccggtag
    14461 atgcaactat agttggagta gtcgattcgt ttgaggtgtt tgatgaataa aagtatagga
    14521 gctatagagt ttaaaagcat tgctaaaggt atagaagtat ccaatgaaat gataaagaaa
    14581 tcttcagttg atgtattgta cttaaaaagt atttgcccag gtaaatttct aataatagta
    14641 ggtggagaaa cttcttatat aaatgaatgt gtagattatg gaataaaact tggagaagga
    14701 tatattgtag ataacttcgt aataaatgca atttcacaag aaatattaga tggatttaaa
    14761 aataagtatc aaaagctaga tagtatagtc tctataggcg ttgttgaaag tagtaaagta
    14821 tgcgctggaa taaaaatgct tgataagact ttaaaatctg gcgacttagt attagtaaaa
    14881 cttcaattat cttttgctat aggtggcaaa ttagtatata tagtagcagg agatttaagt
    14941 agtttagaat atgcattaaa agaaagtgaa aatgtagtaa gagaaaaaga agtaatatat
    15001 aagactgtaa taccatcagt agacagtcaa ataataaaaa gtctaataaa ataatggggg
    15061 gaattatgag tataaacgaa attataatct atgtaatggt agtcttcatg gtacttggag
    15121 ctatagataa atgtataggt aacaaatttg gtcttggaga acaatttgaa gaaggtataa
    15181 tggcaatggg gtcacttgca gttgcaatgg ttggtgttat atgtttagca ccagttcttg
    15241 ctgatgtatt aagaccaatt gtagtaccag tatttaatat acttggagct gacccagcta
    15301 tgtttgctgg aagtttatta gctaatgata tgggtggtgc accacttgca cttgaacttg
    15361 cgaaagaccc taatgctggg ttatttggtg gattaatagt aggggctatg atgggaccta
    15421 caatagtatt tataatacca gttgcgcttg gaataattga aaaagaagac caaaaattct
    15481 tagcaacagg aatacttgca ggtataataa caatacctat tggagcattc gttggtggat
    15541 tagttgcagg attcgaggta atgatggttc ttagaaactt agtgccaata ataatatttg
    15601 cagtagttat agcacttgga ttattaaaat tcgaaaatgc tatgataaaa ggattcacat
    15661 actttggaaa aggtgtagta atagttataa ctttaggact tgctgctgcc atagttgaag
    15721 ctttaacagg aatagttgta atacctggaa tgaaaccaat cagtgaaggt atagaaatag
    15781 ttggagatat agcaatagtt ttagcaggag cattcccatt agtgtttgtt ataacaaagg
    15841 tatttaataa accattaatg gctttaggaa aagttcttgg aatgaatgat gtatcagcag
    15901 caggccttgt tgcaagttta gctaactgta taccaatgtt tggtatgatg aaagatatgg
    15961 acaatagagg aaaaataata aatgtagcat tctctgtatc agcagcattc gtatttggag
    16021 accacttagg atttacggca ggatttaatt ctaatatgat aacaccaatg atagttgcta
    16081 aattagttgg tggtataaca gcagtattgt tagctatggt tatcgcaaat aagacactaa
    16141 agaaggagaa tgtataagat ggatatatca aatatagata aaaatttaat tgaaacatta
    16201 gtaagacaaa taatagaaga aaaaataagt ggaactaaag acacagtaga ttttgttaga
    16261 aataaagaca ttagtggaat aacttctata aagttaccaa ctgtaaaagt tagtgaatct
    16321 gatagattag atacagggaa tccaagtgat gtagtttaca caaaagattt atttacatta
    16381 gaggaaagcc caagattagg ttgtggtatg atggaaatga aagaaacaac ttttgattgg
    16441 actctaaact atgatgaaat agattatgta atagatggaa ctttagatat aataatagat
    16501 ggaagaaagg tttctgcttc ttcaggtgaa ttaatattta taccaaaagg aagtaagata
    16561 caattttctg tacctgatta tgctagattt atatatgtaa catatccggc agattgggca
    16621 tctcaaaatt aataaaaatc aagttaagtt aataataatc aaataaataa tttaaaatac
    16681 aaactcttaa ataagcacac atatttaaaa aagctatgaa ttaaaattca tagctttttt
    16741 tataaagaat gttatatttt agacgtattt tttctatatt tactgggaga catattatat
    16801 tttttattaa ataatctaat aaaatgctct acatttggat aaccaatact attttactga
    16861 taatcttttc cttatcacta atcttacatt ttaaaatatt atttttactc gtaatattat
    16921 cataagaatt tgtttcttat ttaaaactaa ttagaattat aaagtttatt gccatgttat
    16981 taataaaata aatccagtta aatcgagtgc tagttaaagt aataagaaac ttttacaatg
    17041 gtttccttta tcgattgaaa taaaagttaa tatggtataa tatctaaaga gagaatctta
    17101 tcaattagaa tatgcataga aacaagtggc ttaaaaggag acgttatatg gaagagatag
    17161 taaagaagga acaagcaagt atgaatgata tttttttgta tcttaaagct aaatcaaact
    17221 cattatataa atttgtaata ttatataatg agtttatcaa taaacctaaa gattatggaa
    17281 ctggggaact tataaacatg gttgaagtac atatgctaac agcgattgaa gaaaatccag
    17341 gaataagtcc aactcagtta gctaaaatat ggaacagaac taaaggtgca atatctcaaa
    17401 ctatatctaa attggagaaa aagggttata taactaaaga aaagaaagaa ggaaatgcaa
    17461 aaactatatt actatatcca accacatcag gagtagagtt aagtaaggca cataaactat
    17521 atgacaccat agatgttaca agaacatttg aatacttaat caaggagtgt acattagagg
    17581 agatagatag tttttataaa gtaattgatt tgtttattaa tttattagaa gaatctccgc
    17641 aggaaaataa tgattctgaa aaagtgtagc tgagattgaa gttgaataaa gagaaataga
    17701 tattatagaa gtagtactta agtacacatt atataaaaat ataataggta taggattaaa
    17761 aagtttagtg gtttaaagtt taaccactaa acttttttaa tgtctataat ctactattat
    17821 ttaatttact aaaaataaaa aaactcatta tattaagata aaaatagaaa aataactaga
    17881 aaaatactat aatatatcag cttaatacag aaaaaagtta aaaaatttga gaaaaataaa
    17941 aatgaaaacg ttttatattg acatagagaa aaagtagtga taccatttaa atatagttta
    18001 gtagctaaac tatatttaaa ttataataac tattttaggg ggatgttatg aatttaaaac
    18061 aattaagtga aaaatacgaa gaatatgtaa ttcaaatgag aagagaattt cataagtatc
    18121 cagaattatc ttttaaagaa ataagaacaa cccaaagaat aagggaagaa ttacaaaaaa
    18181 taggggtccc ttttgtatct attgaaccaa atatcgtaat agctactata caaggcaaaa
    18241 atgaaggtaa gacaattgca attagaggag atatagatgc gcttccaatg caagaagatt
    18301 cagatgttga atacaaatca gaatatgatg gaataatgca ttcttgtgga catgatgctc
    18361 atggagctat gttactagga cttgcacata tgctaaatga tataagaaaa gatttaaaag
    18421 gtagagtatt attagttttt caagcagctg aagaagttgg tggtggacac aaggagataa
    18481 tacagtactt taaggaaaat ggaggaccag atagattaat agctactcat gtttggtccg
    18541 atattgaagc tggtaaaatt tctgttgaac caggacctcg tatggctggt gggtctggtt
    18601 ggagaataga cataacaggt agaggaggtc atggttctag accagatcaa agcatagatc
    18661 ctataaagcc agcttgtgat atagttttaa aagtatcttc tataccagtt aatcatataa
    18721 gtgtactaga acaatgtgtt gtacatgcat gtgcaataca tggaggaact acaataggaa
    18781 atgttattcc aaacaatgca gaggtaatag gtggatatag atattttact gaagaatcag
    18841 gcaaaaaagt gtttgacatt ataaagcaaa tgagtaatgg tgttggacaa gtttatggag
    18901 tggatgtgaa ggttactcat actggaggca taggacctgt tatcaatgat gaagaagcag
    18961 ttaagatggc taaagaaata gtttctaata tagatggatt ggagttagat tcttatgagc
    19021 caatatgtgc atcagattgt tatggtgaaa tattaaaaga gtattctggc ttttattgtt
    19081 atctaggggt aggtaacaaa gaaaaaggaa taatatatgc acaacaccat cctaagtaca
    19141 atatagatga agatacatta aagttaggtt gcgaatttat ggctaaatat gcagttgaat
    19201 ttttaaataa aaataataac taaaataaat cgaattttat aaatgggggg gatgaaaatg
    19261 caaggtgcat taaaggaaaa taagaaaaaa agtggtggag gatttttagg atggattgaa
    19321 aaagttggga ataaaatgcc acatcctcta gcattattta catatattac attgatagta
    19381 gtagcattat ctgcaatagc aagcttttta ggtttttcag caacaagtcc tgctacagga
    19441 aagttaataa aagttatgaa cttaataagt gcaaaaggtt tagtactatt tatgcaagag
    19501 tttgttaaga acttccaaaa cttccctgtg cttggagtag ttttagttat ggcagttgcc
    19561 acaggagttt gtgaaaagac aggatttttt tcaactgcca taaaaatgag tttatcaaaa
    19621 gttaagggga atgcggttgt atttataata gcttttatag gagtatttgc taatcaagct
    19681 ggtgatgctg cttttgtatt agtaccaact cttgcaggag caatatttta tggattaaat
    19741 agaaatccat tagctggtat attctgtgga tttgcatcag ttggtggtgg atttgcaaca
    19801 gctgtaattc ctgggggatg ggacgttacg ctaacgccaa taactgtatc agctgctcaa
    19861 actattcaac ctgcatttga tatgacatta ttagctagtt attatgcact atttgtatct
    19921 gcgtttatag tagctacagt atcaacaata gtaacggtta aatttataga acctaaatta
    19981 ggtaagtata ctggtaaacc agaaggtata gatgataatc aaggttttga ggtcacaaaa
    20041 gaacaagcaa aagcagcaaa gttagcaggt tttacagttt tagcttatgt agcgttatta
    20101 attgctttgt gtataccagc taatagtttt ttaagaagtc cagaaggttc tttaatattt
    20161 ggagcaccac ttatgtcttg cttacagttt tttatagtaa tactattctt cttgccaggg
    20221 attgtgtatg gaatgagggt tggtaaaatc aagactgtaa aagacttaaa tgatatatta
    20281 tgtcaatcaa tggcagcaat ggcaccgttt atcgtacttg caatagttat aggtcaattc
    20341 ttaacgctat tctcagtgtc taatttagca caggtactag ctataaaagg tggagcatta
    20401 ctcaactcat tgccactacc accacaagct ataatactgc tattcttagt actagtagcg
    20461 tttataaata tatttgttat aagcggaagt acaaaatgga tgatatttgg accagtattt
    20521 gttccaatgt taatgcaatt aaacatacat ccagcattta ctcagtttgt atatagatta
    20581 ggagattctg taaccaatca tttatcacca ttaaatgcat tctttataat acttttagca
    20641 acagtccaaa aatacgataa aaatgcaggt atgggaacta tattctcagc tatgatacct
    20701 tacactatag gttactttgt agtacttgca gttttagtag tagtttggat gacaataggt
    20761 ataccagtag gaataggtgg acaagtttgg ttataaaaca tatttaataa ttttgggagg
    20821 aaagaaaatg gaaacaactg aaataagata caattttgat gaagtaatag atagaagtgg
    20881 aacaaactgt cttaaatatg atcttaagag tcaatttatg ccagaatctc cagaagatgc
    20941 actaccacta tgggtagcag atatggattt tgcatgtgcc cctgagataa ttaatgcaat
    21001 gcatgaaaga atagacagaa aaatatttgg atatagctct caacaaactg atgattatta
    21061 tgaagctgta tgtggatggt ttaagagaag atttgattgg aaaatagaaa aagagaatat
    21121 attctttagc ccaggggttg tgcctgcgtt aggatttttc acacaaatat taacacaaaa
    21181 gggtgatggt atcataatag aaaagcctgt atactatcct tttgaagata aaataactaa
    21241 taatgaaaga aaagtagtta atagtccttt aaagtttgaa gatggtcatt attctatgga
    21301 ttatgatgga tttgaagaat tagctaaaga tgaaaataat aaggtgttta tattatgtag
    21361 tcctcataat ccagttggta gagtatggaa aaaagaagag cttagaaaaa tagtagacat
    21421 atgtaagaaa tatgatttgt ggataatatc tgatgaaata cattgtgata ttattcgtaa
    21481 aggtgaaaag catataccat tagaagtagc atgtcctgat tataaagata gaataataac
    21541 ttgtacagct ccaagtaaga gttttaactt agctggtatg caattatcaa atatagtaat
    21601 caataataag gaattacaaa aaaaatggat gcttgaaaca gattctaaat tagctatatt
    21661 cctacctaat ccattcgcaa tagttgctac taaagttgct tataatgatt gtgaagattg
    21721 gatgaatcaa gtaaatgagt atatagatgg taatattaaa tatgtagaag attttgttaa
    21781 tgagtatatg cctaaagtaa agataataaa atctcaagga acttacttag tttggttaga
    21841 ctttagaggg tatggattag atgcaaaaca gcttgaatat gtaatgctta acaaggcgaa
    21901 ggtattgcta gatgaaggat acatattcgg agaagaagga gcaggatttg aaagaataaa
    21961 tgttgcttca cctagaacaa ttatagaaga gtgtatgaat agaataaaat gtgcatttga
    22021 aggattataa tagtaatatc taaataatat atttgtgtat tttaaaaaaa gactatgcta
    22081 aaaaaatatt tcaaagcata gtcttttaaa gttttatgat gagtttaaaa aatttcaaaa
    22141 cgttactatt cacttatatg tttatattag aaataattcc acaatatact cattttctaa
    22201 agttgcactt ttatacac
//
LOCUS       DAYATN010000054        22119 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.054, whole genome shotgun sequence.
ACCESSION   DAYATN010000054 DAYATN010000000
VERSION     DAYATN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22119
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.054"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(330..521)
                     /locus_tag="ACGI8A_001950"
     CDS             complement(330..521)
                     /locus_tag="ACGI8A_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179947.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            741..1787
                     /gene="cobT"
                     /locus_tag="ACGI8A_001951"
     CDS             741..1787
                     /gene="cobT"
                     /locus_tag="ACGI8A_001951"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFH0179948.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            1819..2379
                     /gene="cobU"
                     /locus_tag="ACGI8A_001952"
     CDS             1819..2379
                     /gene="cobU"
                     /locus_tag="ACGI8A_001952"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFH0179949.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            2389..3162
                     /gene="cobS"
                     /locus_tag="ACGI8A_001953"
     CDS             2389..3162
                     /gene="cobS"
                     /locus_tag="ACGI8A_001953"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFH0179950.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            3182..3796
                     /locus_tag="ACGI8A_001954"
     CDS             3182..3796
                     /locus_tag="ACGI8A_001954"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFH0179951.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      3863..4042
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4157..5671
                     /locus_tag="ACGI8A_001955"
     CDS             4157..5671
                     /locus_tag="ACGI8A_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HFH0179952.1"
                     /translation="MGKKIMLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMAL
                     NSFITKEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSS
                     KYHDYKLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPV
                     IIVGDIDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKV
                     PVIGVVPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVS
                     IRYVDYGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQ
                     MLGKKLKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGK
                     KVKGYEIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEIS
                     FTRAILNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            5710..7086
                     /locus_tag="ACGI8A_001956"
     CDS             5710..7086
                     /locus_tag="ACGI8A_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HFH0179953.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            7087..8058
                     /gene="cbiB"
                     /locus_tag="ACGI8A_001957"
     CDS             7087..8058
                     /gene="cbiB"
                     /locus_tag="ACGI8A_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HFH0179954.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            8090..9160
                     /locus_tag="ACGI8A_001958"
     CDS             8090..9160
                     /locus_tag="ACGI8A_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0179955.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            9162..10049
                     /locus_tag="ACGI8A_001959"
     CDS             9162..10049
                     /locus_tag="ACGI8A_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFH0179956.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            10037..10669
                     /locus_tag="ACGI8A_001960"
     CDS             10037..10669
                     /locus_tag="ACGI8A_001960"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HFH0179957.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            10678..11904
                     /gene="cbiD"
                     /locus_tag="ACGI8A_001961"
     CDS             10678..11904
                     /gene="cbiD"
                     /locus_tag="ACGI8A_001961"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HFH0179958.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDNLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            11901..12509
                     /locus_tag="ACGI8A_001962"
     CDS             11901..12509
                     /locus_tag="ACGI8A_001962"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HFH0179959.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            12499..13074
                     /locus_tag="ACGI8A_001963"
     CDS             12499..13074
                     /locus_tag="ACGI8A_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HFH0179960.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            13067..13819
                     /locus_tag="ACGI8A_001964"
     CDS             13067..13819
                     /locus_tag="ACGI8A_001964"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HFH0179961.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            13803..14924
                     /gene="cbiG"
                     /locus_tag="ACGI8A_001965"
     CDS             13803..14924
                     /gene="cbiG"
                     /locus_tag="ACGI8A_001965"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HFH0179962.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            14921..15646
                     /gene="cobJ"
                     /locus_tag="ACGI8A_001966"
     CDS             14921..15646
                     /gene="cobJ"
                     /locus_tag="ACGI8A_001966"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HFH0179963.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            15643..16395
                     /gene="cobK"
                     /locus_tag="ACGI8A_001967"
     CDS             15643..16395
                     /gene="cobK"
                     /locus_tag="ACGI8A_001967"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HFH0179964.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            16414..17892
                     /locus_tag="ACGI8A_001968"
     CDS             16414..17892
                     /locus_tag="ACGI8A_001968"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HFH0179965.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            17922..18827
                     /gene="hemC"
                     /locus_tag="ACGI8A_001969"
     CDS             17922..18827
                     /gene="hemC"
                     /locus_tag="ACGI8A_001969"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFH0179966.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            18817..20316
                     /gene="cobA"
                     /locus_tag="ACGI8A_001970"
     CDS             18817..20316
                     /gene="cobA"
                     /locus_tag="ACGI8A_001970"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFH0179967.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            20333..21298
                     /gene="hemB"
                     /locus_tag="ACGI8A_001971"
     CDS             20333..21298
                     /gene="hemB"
                     /locus_tag="ACGI8A_001971"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFH0179968.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            21451..22029
                     /locus_tag="ACGI8A_001972"
     CDS             21451..22029
                     /locus_tag="ACGI8A_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HFH0179969.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT         9096 a         2125 c         4168 g         6730 t
ORIGIN      
        1 ttatttgata ttatatttga aaggtagatt ttagtatatt ttgaaaattt tgttttgaaa
       61 atttgggatt atttaagaaa ttttgtatta taaatagaag gaaatagata aattgtatag
      121 aataaaatat attaagataa gttaataatg ttaaatggga agtgagttta tggaaaaacc
      181 aaatacaaaa gttgttaact ttcaagagta taaagacaga aaaaaccaaa gagaagtaaa
      241 tagagacgaa cttttaaagc ttataaaaaa aattgtgact tcaaaatagg gagttgttaa
      301 actccctatt ttaatgggtt tcataataat taagacattc taagtccatc tatataatct
      361 ttgaattgtg agaaatctga ttcactactt aattctatgg tttctgaacc ttccgtataa
      421 aaattcttaa gatagatagt ataattatta tcaacacaat catgataaat acatctatat
      481 ccatcttcat acaaactttt taacttagaa aaatcatcca caatatcatc tccttaaagt
      541 tcttttaaat atatttttac aaaaagcatc taattgtatg caaattgaca atgtaaaata
      601 aaggaaatga acataaaatg tagaataatt tttatatgta tgtaaaaata aaaagaaaaa
      661 gttgaaattt ttagaaaata agagtatttt ttatctaaaa agagagttta aaaaataaaa
      721 aaatgttggg aggataaaaa atgagtttat tagaaagtat atcaaaaaac atatattccc
      781 tagataactc ttctattgaa aaaacaaagc agagattgga taggcttata catccaactg
      841 gaagcttagg aaagatagaa gatatttgca tgcaattagc aggaatattt ggcaatgaaa
      901 attttgacac aagtaaaaag gttataattg cttttgcggg agaccatggt gtatatgaag
      961 aaggcgtagc acctgaccct caaaatataa caaaattaca gtttccaaat ttttcgaaag
     1021 gtctatgcgg agttggagta ataagtaaat ttgtaggggc agatgttgta gctgtagatg
     1081 taggaataaa ctgtgatgaa aaactggatg gggtattaga ctacaagata agaaaaggga
     1141 catcaaatat ggcaaaaggt ccagctatga gtaaacaaga agcaattaga tgtttagaga
     1201 taggtattga aatagcagag cagtgtatag aaaaagatta taaggttatt ggtattgggg
     1261 agatgggtat agcaaatact actccaagta cagcaataat atctgtaatt tcagggtgtg
     1321 acccgttaga agttactggt ataggtgcag gtcttaagaa agaaagacta aagcataagg
     1381 ctgaagtaat tagaaaggct attgaaatta ataatccaaa tccaactgat ggagtagata
     1441 ttttatctaa agtaggaggg tttgaaatag gctcaatggc aggtgttata ttaggttgta
     1501 gtgctaatag gattccagta gtaattgatg ggtttatttc ttatgcagca gcattaattg
     1561 catataagat aaatccaaaa actagagaat atatgatagc atctcattta tcagcagaat
     1621 caggaactaa aagagcatta gacatattaa aattagaccc tctattaaat atggatatga
     1681 gattaggtga gggaagtgga gctgctttag catttgatat aatagaagct tcaaactata
     1741 cttataaaaa tatggcaact tttgatgaaa ttgacatggg aagataaata taaaaattta
     1801 atatgtagga gtgttttgat gagtaaaatt attctagtaa caggaggagc taggtcaggg
     1861 aagagtaact ttgcagaaaa actatgtttt gacagaaata ataatacagc ttatatagca
     1921 acctctattc catttgatga tgaaatgaaa gatagagtaa aaaaacatca agagaataga
     1981 cctagaaact ggaatactta tgaaatatat gaagatatat attccataat taaggagata
     2041 tataaagaac atgaaactgt tatactagat tgtgtaacat tattagtaaa taatctaatg
     2101 tttgaacaca atctaaatat agaagaatct agtcaagaag aaataaacaa attagaacaa
     2161 tacataaaag aacaagttgt caaacttatt gaagaaatag aaaaaacaaa tctatacttt
     2221 gtatttgtaa cgaatgagtt aggtatggga attgttcctg ggaataaact tagtagaatt
     2281 tatgctgata ttgtaggtcg tataaatcaa tatatagctt cgaaaagtaa tgaagtttat
     2341 tttgtagtta gtggcatagc tatgaagata aaggagtagt atgcaattat gaaaagattt
     2401 attttaatac tacagttttt gacaaggata cctataaaat taaatgtagg atttgatgat
     2461 gaattttata agtcaatagt atattttcca cttgtaggtt ttgtcatagg tatattatcc
     2521 tatttaatag gatggatttc tatgctttta tttgaaccat ttatagcttc tataataata
     2581 actctagctg gtgtcttaat tactggagga cttcatatag atggattagg agatacattt
     2641 gatgcaatat atagttatag ggataaagaa aagatgcttg aaataatgaa agattcaaga
     2701 ttaggtacaa attcacttct agcaattatg tttgtacttt tattaaaggt tggattcgtt
     2761 tacgatataa taagtaataa ttcattgtgg gttataattt ttatgccaat gattgcaagg
     2821 ttaggagtaa tgttattgac atataaaact gtgacaccaa gagaaaaagg aatgggaaat
     2881 ttatttatag gaaaattaac tacaagtatg ttaataactg ctataatata cacattattg
     2941 atagttgctc ttattactaa atttatattt ttattaccta atatagtgtt gattaaagta
     3001 ttaggttcga ttatagtggt ttttgtattt ataatattat tcaaaaaaca tatttataaa
     3061 aaaattgacg gagtaacagg agatatatta ggctgtggaa tagaactttc agagcttgta
     3121 tatttaattt acatatattt gttaattttt atgttttttt aaagtaaaaa gtggtgataa
     3181 tatgataaga ttaatattaa taagacatgc actgacaaat gataataaaa aaggaagatt
     3241 atcaggccat ataaatagtt gtattagtga agagggcaaa ctacaaataa ataaaattac
     3301 cagatatctg agtaatgaaa acatagataa ggtttataca acaccatcta cacgtactaa
     3361 agatacagta gaaaaaatat caaaattgaa attattagaa atagaagaaa aagaagcttt
     3421 aagagaaatt agttttggag actttgaagg aagaactttt gaagaaatta aaatcaaata
     3481 tccaaatgag tttgaaaaaa tgataaaaga aggtaacaac tatagatatc caaatggaga
     3541 aagtttgata gactcttata aaagagtggc aaaagaaatt gataatataa ttttagaaaa
     3601 taatagtaat ttagatacaa agacaatact tatatgttct catgcaggaa caataagaaa
     3661 tataataaca catttgattt ctggtagtta taagtatcac tggaacttta aaattgataa
     3721 tgcatctatt acagttttag aaattgatgg tggatttgct gtaatagata aaatgaattt
     3781 tacagacttc atataattta atcaaaacca aacattacaa tctgtgtttg taatatatct
     3841 gtaaaattag caatgaaaat agaatattag attaaaatta ggttcttaat tagatgttaa
     3901 aagggaaaaa ggttagatgc ctttgcagcc cccgctactg tgaaaccaac gaaactataa
     3961 ataccactgt cagtttgatg ggaaggttat agaagtagga tgaagttaag ccaggatacc
     4021 tgcctaattt aatttaaaac aagaggtctt cggggtgaaa gatttttaat taatatatat
     4081 aaaagtccta tctttagaag tattaggact ttttttaatt tgttttttag taaattttaa
     4141 caaggaggga tggaaagtgg ggaaaaaaat aatgctacaa ggaacagctt caaatgtagg
     4201 taaaagtgtc ttagtagcag gtttgtgtag aatatttaag caagatggtt atactgttgc
     4261 tccttttaag tcacaaaata tggctttaaa ttcttttata actaaagaag gattagaaat
     4321 gggaagagca caagtatttc aagcagaggc tgcgaaaata gagccaatag ctgatatgaa
     4381 tccagtttta ttaaaacctt ctggaaataa taaaagtcaa gttattgtaa gaggtaaagt
     4441 tgtaggagaa atgccttctt ctaagtatca tgattataaa ttggagttag tagatgtgtt
     4501 gaaggaaaca tttaatagct taaattcaag gtatgatatt gtagttatgg aaggggcagg
     4561 aagtaatgca gagataaact taaaagatag agatatctct aatatgggta tggcagaaat
     4621 agctgatgca cctgtaataa tagtcggaga tattgataga ggaggcgtat ttgcctcatt
     4681 agcaggaaca atgcttttat taaatgaaga tgagaaaaga agagtaaaag gtgtaataat
     4741 caataaattc agaggaaaaa aagagctttt acaagatggt gtaaagatgc tagaagacat
     4801 cataaaagta cctgttatag gagtagtacc ttatacagat ataaaaatag aagatgaaga
     4861 cagtgtaact acgagattta agaaaaaaat gaataaaagt gatatacaca tagaaataat
     4921 tagaacccca catatgtcaa attttacaga ttttaatata tttgagacac aagaagatgt
     4981 aagtataaga tatgtagact atggagaatc tttaggaaat ccagatatat taataatacc
     5041 tggaactaaa agtactatag acgatttaac atatataaga aaaagtggtc ttgaatcgca
     5101 aatcaaaaat ttacatagtc aaggtaaatt gatatttggg atatgtggtg gataccaaat
     5161 gttaggtaaa aaattaaaag acccttatca tgttgaaagt gaaattgaag aagttgatgg
     5221 aataggtctt ttagatacag aaacaacgtt tgagaaagaa aagactacaa cacaagtaga
     5281 agctactatt gttgaatgta tcagtgaata catgaataac cttaagggaa aaaaagtaaa
     5341 aggttatgaa attcatatgg gaattacaaa gcgaggtaat gatgtaagaa gtttagattt
     5401 aataactaaa aaacttgata aagaagtgaa ttatactgag ggaagtgtaa ataaagaggg
     5461 gaatgtaatt gggacttatc ttcatggaat atttgatgaa ataagcttta caagagcaat
     5521 tttaaacaat ataagaaaga aaaaaggttt aggagaatta gaaagcaaag taaactcttt
     5581 taatgaattt aaggataaag agtatgataa gttggctgat tttttaagag aacatttaga
     5641 tatagagaaa atatatgaga ttatgaaata atgatgacct ttatttataa tttaggtagg
     5701 aggtgtatta tgaaaaaaat tttaattgcg ggtactaata gtggagtagg taaaacaaca
     5761 atatcattag gaataatgca agctttggtt aagagaaata tgaaagtaca gccatataaa
     5821 gtagggccag actatataga cccttcatat cacactttta taactggaag acattctaga
     5881 aatttagatt cttatatgtt agatgatgaa aaaataaagt atatagtaaa taagtcctca
     5941 aaagatgcag atatttcagt aatagaaggt gttatgggct tatatgatgg ctttggaata
     6001 gatttagata attgtacaag ttcacatact tcaaaggtat taaagtcacc tgttatatta
     6061 gttataaatg gtaaatctat ggcagcatct agtgctgcta tggtacttgg atataaggaa
     6121 ctagataaag atgtaaatat aaagggtgtt atagtaaata atgtaaagac taatagccat
     6181 tatcaaataa ttaagagttc tatagaaaaa tattgtaatg ttgaagtatt ggggtatttt
     6241 cctccaaata acaaattctc tttggaatct cgacatctag gattagttcc tagtgtagaa
     6301 atgaaatcac taagggaaaa attttatacc ttagcagatg aaattgaaca ctatatagat
     6361 attgacagaa taattgaaat atcagaaagt gatgaatttg agagtgattt tgattttcaa
     6421 actttgatag aaaataataa aataaaagaa catgttaata ataaatcaat agctatagca
     6481 tatgataaag cattcaattt ttattataga gaaaatatag agcttttaga ggaacttggt
     6541 ttagaaataa actattttag ccctcttgaa gatgtatcag tgccaaatgc agattatatt
     6601 tatattggag gaggttttcc agaaatattt ggaaaagaat tagaagcaaa tgagtcaatg
     6661 agaacttcca tattagaagc acataacaat aatataccta tttatgcaga atgtggagga
     6721 ctgatgtacc taggtgagaa actattgaat caagaagaac aagaatttaa tatggttgga
     6781 atatttagtg gaattagtaa gatgacatct tctttaaaga gatttgggta ctgtattggt
     6841 gtagccaagg aaaatactct tttatctaaa aaaggggaat ccattaaagg gcatgagttt
     6901 catcattcaa tatttgaaag tgatgaaaaa tgtgtatatt caatgagaaa agaaagagat
     6961 ggaaatatag tagaagagtg ggatggtggt tatagtaaag gaaacacttt ggctacatat
     7021 cttcatactc atttttataa taacttaaat tgtatagaaa attttttgaa aaaaggaaat
     7081 gaataaatga gtattttatc tatttatata ggatatgtga cagacttaat tgtaggagac
     7141 ccatattctt ttcctcatcc agtgagattt attggtaaac ttataaattt tactcaaggt
     7201 ataataagaa aggtctctaa aaatgatagg cagttaaaat ttgggggatt tatattatgg
     7261 tttataaccg ttggaataac atatttaaca acttatgcta tagtgaagtt atttagtttt
     7321 aatatattat tgagtattgt agtgaatagt tttataattt atacaacact agccacaaaa
     7381 tgtttaaaag acgaagcatt aaaaatttat aatgtattaa aaactgaaga tatagaaaaa
     7441 tctaggattc agctatctta tatagtaggt agggatacta ctaatttaag tgaacctgaa
     7501 ataataagag caactgtaga aactgttgca gaaaatactg tagatggtat aattgcgcct
     7561 atgttttatg catttattgg aggagcacca cttgctatgg cctataaagc tatcaataca
     7621 ttggactcaa cagttggata taaaaataaa aagtataaag atattggttt tgcatctgct
     7681 aagattgatg atatagccaa ttatatacca gctagaatat ctgtcattct tatgacaatt
     7741 ggaagtttct ttttaaatta taactatagg aactgtttta aaatctctat tagagataga
     7801 aagaatcata aaagtccaaa ttgtgctttt tcagaaggtg cagtatctgg tgctctagga
     7861 atacaactag gaggtaccaa cgtttatttt ggggaaaaag tatataagcc aacaataggg
     7921 gataagctta gggagataga taaagaagat ataattaaaa ctaataaaat tatgtatgca
     7981 tcttcatttg tatctatgtt ggtatttaca ctaatatatg tgttatgtag gatgatttta
     8041 attaacttaa ttatataaat tattgtttgt taacttaagg gggacataga tgaaggattt
     8101 agggcatggt gccaatgtag atgaaatggc aagattatat gggaaaaacc caaaagaaat
     8161 aattgatttt agttcaaaca taaatccaaa tgtattacca aatttagaga gatacatttt
     8221 aaaaggatta gaagagtgta ggaattatcc agatataaat tatacaaact tgagagaaaa
     8281 tatatcaaaa tatatagata taaatccaga ctttataata cctggcaatg gtgcaacaga
     8341 agtgatttat ttgcttatga agagtattaa aaaaaaattg gctataataa atccgacttt
     8401 ttcagagtat agaagaagtg ctgagttaaa taatttagat attatagatt tagagttaga
     8461 tttagaaaat aactttaaac ttaatataga tataataaaa gaaaatataa aaagatttga
     8521 tagtttattt atatgtaatc ctaataatcc gagtggtaat gtccaagatt taaaggaact
     8581 tgttcattta ctagataaac ataataaggt actaataatt gatgaaactt tcatggaatt
     8641 tgttgaagat gaaagtaaat atagtttggt taaatatata gaatcaaata aaaatatatt
     8701 tataattaaa gctgtgacta agttttttgg tatgcctggt ttaaggctag gatatggtct
     8761 tacaagcaac actgaaatta tgaacaaaat ttatgaacac aaagagccat ggactatcaa
     8821 ttcttttgca gatatactat ccaattttat ttttgaagat aaagagtata ttaaaaatag
     8881 taaagaatat tacatagaag aaagaaaata tatgttacaa gagttgagaa acataagaaa
     8941 tataaaggtc tatgatacag atgctaattt tatcttgata agaatatata aaaaaacaac
     9001 taaagaattg aaaaaagatt tgtttaagca aggaaacata ttagttagag atgcatctaa
     9061 ttttatagga ttagatgata gctttataag agtggctata aaatctcatg aagataacaa
     9121 aattcttata gaaaatatga aaaatttatt gggtgattaa aatgaaatct tatggaatat
     9181 gcccagcatc ttgtggagaa tttgtacaag gtataattga tgatgaagaa tatttatgtt
     9241 catatgcaat agatatgtat tctaaagtgt atattgaaga aaaattagta gatataaatt
     9301 tagggagata taagtcaagg ttagcaattg aaaaggtatt tgaaaaattt aatctaccta
     9361 aaaaatatac taaaaatatt tctttaaata taaatagtaa aatacctgtt ggaaaaggaa
     9421 tggcaagttc aactgctgat ataggagcta ctataaaggc aacattatca ttgatagata
     9481 aagatttaag tagcgaggag atttctaaat tagcagcaga aatagaacca acagattcta
     9541 tatttataga taaaaatagt atatttaatc ctctaaatgg aactgttata aaatacctag
     9601 gaaatctgac caatgcgaaa gttgtaatac ttgaaccaaa caaagtacta gatactatga
     9661 aaattagatt gaggcaagat tataataaat taaaagtaga gaataaagaa gtaataaaaa
     9721 aatcatttgc tcttttagaa gaaggattga aaaaaaataa tttatctcta gttggagagg
     9781 cttgtacttt gagtagctta gcaaatgaga atatagaaaa aaaagagtat ttaaatgaaa
     9841 taataaaaat atcaaaaaaa tatggagcat atggagtaaa tattgctcac agtggaactg
     9901 tagtaggtat tttaattgat aagtttatga atgataaaaa gatgatagat gccttatgtg
     9961 aatctaacat aaattctgtc tataataaaa tttatacaca aaacataatt aatggaggaa
    10021 ttaaggggga aatagaatgg aatacataaa gaatcctatg aaaatagaag aaaaaagttt
    10081 tgaattgata caagaaataa tagacgaaat tagaccagat tataaattta agaataatat
    10141 cgaagaaaaa ataataaaaa gagctataca tactactgct gattttgatt acttagatat
    10201 attaaaaata tcagaagaag cagttgatag tataatagat gcattaaaaa ataatgcaag
    10261 tatttatact gatacaaata tggctcttag tggtataaat aaaagaaagt tagaatcatt
    10321 aggatgtaag tataaatgtt tagtggcaga tgatgaaact gtaaaattag ctaaagaaaa
    10381 aggaattact cgttctatgg cagcagtaga aattgcagct aaagaagaag gaagaaaaat
    10441 attcgtttta ggtaatgctc caacagcttt atataaagtt atggagatga aatcagaagg
    10501 tagattagat ttagatgcag ttataggagt tcctgtaggt tttgtaggag cacaagaatc
    10561 aaaagatgaa gttgaaaaaa cagatatacc atatataatt tctaaaggta gaaaaggtgg
    10621 cagtaattta gcagcagcaa tagtaaatgc tatattatat actatgtagg tacctatatg
    10681 gaagaatatg tatatataga tggaaaaaaa tatagaagag gatatactac aggttcatgt
    10741 gcaacaggtg catctaaggc tgctgtatat atgttgatta caaaaaatag aatcaatact
    10801 ataaatatag atactcctaa ggggatacca ttactactaa aagttgataa tataaatatt
    10861 tctgatacat ttgtggaatg ttccataaaa aaagatggtg gagatgatat agatgcaact
    10921 cataccatgg atatttatgc aagagctgaa attgtagcta aaaatgataa gaacaaaggg
    10981 tatcttactc taaaggatat agataattta agcaccaata gtgagtgtaa aagtgagctc
    11041 tataagttta ttagggttta tggcggaact ggtattggtg tggttacaaa aaaaggtctt
    11101 agcgtggatg ttggtaaacc tgctataaat ccgactccac ttaaaatgat aaatcatgaa
    11161 ataagaaagc ttataggaga taattttgaa tctatacttg gaaatgataa agttttaaaa
    11221 ataacaatct ttgctccaca aggagaaact gtagcaaaaa aaacatttaa tccaagatta
    11281 ggtatagttg gaggaatatc tataataggt acaactggga ttgttgaacc tatgagtgat
    11341 gagggttgga agaagtctct atctatagaa cttcagatga aaaaagaaca aggcttagat
    11401 aaaataatat tggttccagg aaatcatgga gaacagttta taagagaaaa attaaactta
    11461 gatattaagt atgtagttag ggtaagtaat tttataggct atatgattaa agaagctcaa
    11521 agaataggat ataagaaaat cttaatggca ggtcatatag gtaaatttat aaaagtatct
    11581 gctggtattt ttaatactca tagtaaggta gcagatgcta gaagtgagat attagtggca
    11641 aacttagctt taatgggagc tagatacgaa tttttaaata aaataaatca atgcgtaact
    11701 actgaagaag ctgtagagct aataaataat agtgaatata gagaagtata taatatatta
    11761 agtaataaat gtagggagag agtaaagcaa tacttgaatg aagattcaga tgatattgat
    11821 gtggaagtaa tcatattttc aatggataag tctttattag gtaaatcgga taatacagat
    11881 gatttagtgg aggtctttat atgataaaca taataggtct aggacctggt aacttagact
    11941 atataacaaa aaaaggtgaa aatttaattt ccacttctga tgtattaatt ggtggtaaaa
    12001 gaaatttaga gtctataaaa aattttgaag gagaaaaaat agttctggat tctaatttaa
    12061 gagaaattat agagtacata aataacaata aagaaaagca aatatcaata atagcatctg
    12121 gggacccact aatatatggg ataggtagat atctatctaa aaatattgat aataagatgt
    12181 taaacatggt ttctgggatt agttcattac agtacatatt ttcaaaaata tatgtagata
    12241 tgaatgatgt atatattaca agtagtcatg gtaaagtacc agattttgat tacatattat
    12301 cacataaaaa agtatgtatg gttacagatt caaagatagg accaaaacaa ataagtagag
    12361 aaataataga tagaaatcta aataaaatta ttgttgtagg agaaaatctg tcatatgaca
    12421 atgaaaaaat aacaatagca aaaccagaag agataataag aatggacaat tttgatatga
    12481 atgttgtggt gattttagat gaggaatagt gaatttataa caggtaaagt tccaattact
    12541 aaggaagagg ttagagcaat atcaataagt aagttaaatt tagtaaatgc taaaaacctc
    12601 atagacatag gggctggtac aggaagtgta agtgttgaag ctgcatataa ttatcctaat
    12661 ttaaaggtta tatctataga gaaaaatgat gatgctatag agcttataga gaaaaataaa
    12721 aaaaaattca acttgaaaaa tatagaagtg ataaaaggat atgctccaat taatctaggg
    12781 ttaaatgtta aagttgagtc tatttttctt gggggaacag gaaataatct ggaagaaata
    12841 atagaatggt ctaaaaaaaa tctcgttaca ggaggaagac ttgtagctaa ttttataata
    12901 atagatacat ttaatcaaac tttaaaactg cttaaaaagc atggatttaa agaaatagat
    12961 gtatgtgttt tgaatgtttc taaactagag aaactaggaa aaggtgaata ttttaaacca
    13021 ctaaatccta tatatataat atcttgtgaa aaaggggaaa atgatgatga ataaagtaca
    13081 ttttgtagga gcaggacctg gagataaaga gctgataaca ttaaaaggat acaaattatt
    13141 aagtaatgca gatgtagtga tatatgcagg ttcacttgtt aatcctgaac ttttggaata
    13201 ctgtaaagaa gattgccaaa tacataatag tgcacatatg gatttacaag agataataga
    13261 tgttatgaga gaaggtatag aaaataataa atctgttgta agactgcaaa caggtgattt
    13321 ttctatatat ggttcaatta gagaacaagt ggaagattta aataaattga atatagatta
    13381 tgactgtact cctggagtaa gttctttttt aggggcagca tcatctttag gggttgaata
    13441 tacagttcca gaaatttctc aaagtgtaat tataacaaga atggaaggca gaactccggt
    13501 tccagagaaa gaatcaatac aatcatatgc taagcatcag acatctatgg taatattttt
    13561 atctgttcaa gaaattgaga aagttgtgtc taaattactg gaaggtggat atccaaaaga
    13621 tactcctata gcagttatat acaaagcaac ttgggcagat gaaaaaatag ttaaaggaac
    13681 tttaagtgat atagctgtaa aagttaaaga aaacaacata aataagactg ctttaataat
    13741 ggttgggaga tttttaggag aagagtataa taattcaaag ttatatgata aggatttcaa
    13801 acatgaatat agagggtaat attgctataa tctgtattac agaaaatgga aaaaaattag
    13861 ctctaaagat aaattctcta gtaaataact gtgttatata tcaagtaaaa aataaaaaaa
    13921 gtaatttagt tgttgaagaa acatgtgtgc atacagttca aaagaagtta agtgattttg
    13981 taggagaaat atttagcaaa tttgattaca tagtatttat aatggctaca ggtatagttg
    14041 tacgaagtat agcaccatac ataataagta agtttagtga ccctgcaatt ttggtaatgg
    14101 atgaaaaagg caaaaacgta ataagtttat tgagtggtca tattggaggg gcaaacagct
    14161 tgacacagta cataagtagt cttattgatg ctaatccagt aataacaaca gctactgata
    14221 taaatcaaaa agcttcctta gatatgatag caaaaaaact aaatgcacat attgataatt
    14281 ttagagaaag tgtaaaagaa gtaaattcta tgctagtgaa taataaatgt gttggattat
    14341 atgttgatga aggatatcaa gttgatacta ggggatttaa acttatagaa agtaaggatg
    14401 agttaagaga tagtttttca aacacatata tagaaaaatt agttgtagta agcaataaaa
    14461 taaattgtat tgaagatatt gagaatttaa acctagataa taaatttgaa cattgctata
    14521 gtacccaaaa aataataaag gtaattccaa gagatatagt gataggaatt ggctgtagaa
    14581 gaaacacaga tagtgaactg atgtataatt cacttgaaga gtttcttaat aaacaaaata
    14641 tagatattag agcaataaac acagttggaa gtatagaact gaaaaaagac gaaaaagcaa
    14701 taattgaatt atcaaaaata ttgaaggtgc catttaaaac attttcagta gatgaaatat
    14761 ctaaggttga taatcttttt gataaatctg attttgttaa aaaaaatgtg ggtgtgtatt
    14821 cagtagcaga acctgtagca tatcttttga gcaaaggtaa tttgttagtt gaaaaacata
    14881 aatataaagg aataactttt tcagtgggga gaataaaaat atgatatacg ttgtaggaat
    14941 tggaccagga agtaaagata caatgacttt agaagcaata aaagcaatag aagattctga
    15001 ggctattgtt ggatataaga cttatataaa actaatagaa gaatttatac aaggtaaaga
    15061 aataatccaa aatggtatga ggcaagaagt tgatagatgt aaacaagcag tggaggaagc
    15121 aaaaaaagga aaaaaagtag cagttgtaag cagtggtgat gctggaatat atgggatggc
    15181 aggtcttatt ttagaattaa tatctaaaga agatgaagat ataaaagtaa aggtaattcc
    15241 aggcgttact gcaagtattg gtgcagcagc tatacttgga gctcctatta tgcatgattt
    15301 ctgtcatata agtttgagtg atttaatgac tccgtgggaa gtaattgaaa aaagattgag
    15361 attggctgct gaagccgatt ttgtaatttg tttgtataat ccaagaagta agggaagaag
    15421 tgaacatcta agcaaagcat tcaaaataat gggtgagttt aaaggtggag atacacctgt
    15481 tggagtggta aaagatgtag gtagagaaaa agaagaaaag tttgtatgta cttttgaaaa
    15541 tatggacttt gaaaaagtcg atatgactac aatggttata ataggaaata agtctaccta
    15601 tattaatggt gaattgatga taactccaag aggttataca gtatgatttt aattttagga
    15661 ggaacttcag atagtttatc tatatgtgat aagataaatg aactgaaaaa tcaaccatat
    15721 atcctttcag ttactacaga ttatggaagg gaacttgcac aacaacatgc agagaatgta
    15781 attttaggta agcttggaaa agaagatatg ctaaaattta taaaagataa taatatagta
    15841 aaaataatag atgccactca tccttatgca gttgaagtat ccaaaactgc tattacttgt
    15901 gcaaaactat tgaatataga ttatataagg tttgagagaa aatctttaat tgaagaaata
    15961 gattatgata ataaatttat agtagataca attgaagaag cctgtgaaat agcaaataaa
    16021 atagggacaa atatattcat aggaacagga agtaaaaatt tgaacagatt tatagaggaa
    16081 ataagtgata aaaatttagt tgttagagtt cttccaacga gtgaagtcat attaagttgt
    16141 gaaaaattag gtttaagtgc agataatata gtagctatga aaggtccatt tagccagtat
    16201 ataaatgaag aaacatataa acattacaat atagatttag tcataacaaa agaaagtgga
    16261 atagctggtg ggttcttaga gaaggtaaat gcatgtaaaa ctttcaatat accagtaata
    16321 attataaaaa gagaatcgat taactacgaa aatgtattta atgacataaa taaaattaaa
    16381 gacatacttg tgtaattttt aggaggagaa tatatgaaga aagccttatt agtagtgagt
    16441 tttggtacaa gctatcatga aacgagagaa aagacaatag atatatgtga agataaaata
    16501 aaaaattcgt taaaaagcca tgattttttt agagcttata catcaaatat gataataaat
    16561 aaaataaaaa aaagggatgg aatagaaata gataatccaa tacaggcttt agataaaata
    16621 tatgaacaag gatatgatga agtaataatc caaacacttc atattatatg tggcgaagaa
    16681 tttaacaaat taaaagaaca agtagatgga tattcttcta aattcaaaaa gattgtatta
    16741 ggaagacctt tgcttacaca tattgaagat tatcaggaag ctgtagaggc aattaagcat
    16801 caaattcctc atatggaaag taatgaagca gtagtattta tgggacatgg aacacttcat
    16861 gaatcacatt cagcgtatcc agcattggaa tacatgctta gggataatgg aataaatgct
    16921 tatgttggta cagttgaagg gtatccagaa atagaacatg taataagaag attaaaagaa
    16981 ggaaatataa aaactgtaaa tttgatgcca tttatgttag ttgcaggaga ccatgctata
    17041 aatgatatgg caggagatga agaagattct tggaaaacaa tattagaaga aaatggattt
    17101 aacgtaagag ttcatttaaa aggactagga gaaaattctt atatacaaga taaatttgtt
    17161 cgtcatgctg taaaatgtgc agagaatgcg aagttctatg gtataggagc agggccagga
    17221 gatggttctt tacttacgat aaaagcggtt aatactttaa agaaactgga tattctatac
    17281 actccagaat caaaaaaggg tggagatagt ctagctttat caatagttag tgaatattta
    17341 cctgaatcag tggaaataaa gtctaggcat ttcccaatga gttttgatgg gaatgaaaaa
    17401 agtttagctt gggacaatgt ggcacaagaa attatatcag atgttaatga tggtaaaaat
    17461 gtaggatttg ttacattagg agaccctatg atttatagta catatgtata tataatgaaa
    17521 agaatattag gagaggtaga agtcgaaact ataccaggaa tttcatcatt ttccaatata
    17581 gcatctaatc agaattttcc attagtcatg gataaagaag ctttagtggt aattccttgt
    17641 actatggaag atgataagat agagtatgct ttgcaaaatt ataattcaat agtattgatg
    17701 aaagtatata aaaattttaa agaaataata aaaaaattag aaaagtatga tttaatagaa
    17761 catgctattt tagtaagtaa ttcttcccaa gaaagcgaaa atgtgtttac agatttaaaa
    17821 gaagcgcacc ttgaagataa gatatcttat ttttctacta tactagtaaa taaagataat
    17881 aaaataaaat aggtgaaagc aatatttaaa ggagacttaa tatgaatata gtggttggaa
    17941 ctaggggtag taatctagcg cttatacaaa cagaatgggt aataaatgaa ttaaaaaaga
    18001 agtatccaga aattagtttt gagattaaaa tcataaagac aaaaggagat ttaattcaaa
    18061 atgtttcact ggataaaata ggtgataaag gattgtttgt caaagaaata gagcaacaat
    18121 tattagatgg aaaaatagac atagcagtac atagtatgaa agatatgcct tcttatttag
    18181 cagatggact aaaatttgct catacgccta agagagaaga cccaagagat gttctaatac
    18241 ttagagaagg atataaaaat ttagatgatt tgcctcatgg agcagtaata ggaacaggaa
    18301 gtaaaagaag gaagtttcaa ttattgaaac aaagacctga tttaaatata gtacaagtta
    18361 gaggtaatgt ggaaactagg atacgaaaaa taaaagatga aaacatgcat ggtattgtgt
    18421 tggcagcatc aggaataatt agagctaatt tacaagataa aataagttct tatttacctg
    18481 ttgatgttgt tattccagca cctgcccaag gagctttagc tatagaaatt agaagcaatg
    18541 atagtacgat agagggaata gtaaattcac tgaaggatga gaatacagaa atccaaatat
    18601 tggctgaaag aggtttttta gatggtgtaa atggaagttg tcatattcca atggcagctt
    18661 attgtgaaat aatccaagat aaaatacatc ttactggctt atatggagat tcagaaggaa
    18721 aaaaagtagt aatcaagtct atagatggag atatttcttc tcctagagag ttaggtttaa
    18781 agttagctaa acttgtattg aaggagtatg agaattatga agggtaaggt ttacttagta
    18841 ggggcaggac caggtgatta taaactcatg actttaaaag gtttagagtg tataagaaaa
    18901 tcggatgtaa tagtatatga tagattagca aatagtagtt atttaagaga agctaagcct
    18961 gattgtgaac ttatatatgt aggcaaggca tcaagtaatc atattttgcc acaagaagat
    19021 ataaatagaa ttatagcaga aaaagctaaa gaaggaaaaa ttgtaactcg tcttaaagga
    19081 ggagaccctt atgtatttgg cagaggggga gaagaagcag agactttagt agatgaaggt
    19141 atagaatttg aagtagttcc aggtataact tctgctatag gaggtttatg ttatgcgggt
    19201 attcctataa cacatagaga ccatgcttcg tcatttcatg taataacagg tcatttgaag
    19261 ggtgatgata gtggagaatt aaactggaat gctcttgcaa ataacaaagg tactcttgtg
    19321 tttttgatgg gaatttcaaa cctagagaaa ataagtgaaa acttaatgaa agaaggaaaa
    19381 gataaggata cacctgttgc tcttataagt tgggcaacta gatataatca aagagttgtt
    19441 acatctacac ttgaaaatgt ttatgaaact gctattaaag aagaagtaaa accgccaact
    19501 cttatagtag ttgggagtgt tgtaggactt agagaaaagt tgaacttctt tgaaagtaag
    19561 cctttatttg gaaaaagtat tgctgtaact aggtctagaa atcaaaatag tgtattggta
    19621 gaaaaaataa tggatttagg tggaaatcct atagaaatac ctactattaa ggtagaaaaa
    19681 atacaaaata atattaactt agaaaatgaa ataaaaaata taaataagta taattatcta
    19741 atactaacta gtaaaaatgc cgtagaaatc ttctttgaaa agatatatga aatgaatttt
    19801 gatttaagga tattatctaa tttaaaggta tgtgctatag gttctgctac atcaaatgag
    19861 ttgaaaaaaa gaggaattat agcggatata gtaccaaaaa aatttgtagc agagtcactg
    19921 tatgaagagt tagcgcctat tttaaatgat gacgataata tacttatacc tagagcagaa
    19981 aatgctagag attatctggt tgacaaactc aaggctattt gtgaagtaac agaagtacat
    20041 atatatagaa cagtaataga tgaggagaaa aaaggtgaga tattagatat tttaaattct
    20101 aatgacatag attatataac atttactagt tcctcaacag taaataatct tgttgaaatt
    20161 ataggaaaag aaaatattaa aatattagaa aaaataaaaa cactatctat aggtcctata
    20221 actacagaga caataaatag tttgggaata aaattgtaca aagaatctgc tacatctact
    20281 atagattcgt tagtagattt aattataaaa aattaatttt ggaggcttga atatgttaag
    20341 aagaccaaga agactaagag caagtaaagc aatcagaaat ttggtaagag aaactaaggt
    20401 gaatgtagaa gacttaatat atccactgtt tgtagtagag ggggaaaata taaaagaaga
    20461 aattaaatct ttaccagatg tctatcattt atcaatagat atgctagaag acgaaataaa
    20521 agaaataagt gagttgggaa ttgaacatat aatgttattt ggtgttccag atgaaaaaga
    20581 tgagtgtgct tcacaagctt ttaatgataa tggaatagtt caaaaggcaa ttagaaaggt
    20641 aaaagaaata aattcagata taaatgtaat aacagatgtc tgtatgtgcc aatatacaag
    20701 tcatggacat tgtgggattt taactgatac tggttatgtt gataatgata aatcattgga
    20761 gtatttggct aaaatatctg taagccatgc taaagcagga gctgatatgg ttgctccatc
    20821 tgatatgatg gatggaagaa tacaagcaat aagagaagct ttagatgaaa atggattaga
    20881 aaatgttgga atcatggctt atagtgttaa gtatgcttca aattattatg gaccttttag
    20941 agaggcagct cattcagcac catcttttgg agatagaaaa acatatcaaa tggacccagc
    21001 aaatagcaat gaagctataa tagagactga acttgatata ttggaaggtg cagatatact
    21061 tatggttaaa cctgcattat catatttaga tattataaga agagttaaag ataattttga
    21121 cttacctcta gcagcttata atgtaagtgg agaatactct atgcttaagt ttgctgtgaa
    21181 ggaaggttta ttaaatgaag atgtaattct tgaatcaatt acatctataa aaagagcagg
    21241 agcagatata ataataactt attttgctaa agacattgct aaactgttga aaaaataaat
    21301 tctagcagta taaagaatat caaaaagtat aggaattgta attaactatt atacagtagt
    21361 tttgtttaga catttattga agaataatag aaactaattt gcaatttcta tactttattt
    21421 atgtaactaa ataaaatttg gaggtagaag atgctatatc ctattaattt aaaattagat
    21481 gagttagatg tagttataat tggtggtgga gaagttgcat ataggaaatg taaaaatttt
    21541 ttagagttta acaaaaatgt tactatagtt tctaaacaaa ttttaaataa attttatgat
    21601 ttaaaaggaa atataaaaat aataaaagat gattataaag aaatatatat aaaagatgct
    21661 tctgtaataa ttgctgccac aaataataga gagttaaata tggaaatagg attatattgt
    21721 aaagcaaaaa ataagctagt gaatgtagtt gataatatag aaatatcgaa ctttactgtt
    21781 ccatcatata taaaaagagg agatttactt ataagtgtgt ctactggggg caagagtcct
    21841 tcattatcat caaagataaa aaaagaaatt gaagagagat atactgaaga ttatgaggag
    21901 tatttaaatg tattaggaga tataagaaaa aaggttataa aaaaatacga agataaaagt
    21961 aaaagaaaag atgtgcttaa tatgttggta acacttgacc tagaagaact taagaagttt
    22021 gatatataaa aaataaaata aaaaatgtta aaaaggtgtt gacggattat ttttaagatg
    22081 atatagtatt acttgtcctt aagaaaaggg caaacataa
//
LOCUS       DAYATN010000055        21476 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.055, whole genome shotgun sequence.
ACCESSION   DAYATN010000055 DAYATN010000000
VERSION     DAYATN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.055"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(22..900)
                     /locus_tag="ACGI8A_001973"
     CDS             complement(22..900)
                     /locus_tag="ACGI8A_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFH0179970.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(917..1234)
                     /gene="trxA"
                     /locus_tag="ACGI8A_001974"
     CDS             complement(917..1234)
                     /gene="trxA"
                     /locus_tag="ACGI8A_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFH0179971.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1384..2616)
                     /locus_tag="ACGI8A_001975"
     CDS             complement(1384..2616)
                     /locus_tag="ACGI8A_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0179972.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2606..3277)
                     /locus_tag="ACGI8A_001976"
     CDS             complement(2606..3277)
                     /locus_tag="ACGI8A_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0179973.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3293..4129)
                     /locus_tag="ACGI8A_001977"
     CDS             complement(3293..4129)
                     /locus_tag="ACGI8A_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179974.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4155..4829)
                     /locus_tag="ACGI8A_001978"
     CDS             complement(4155..4829)
                     /locus_tag="ACGI8A_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFH0179975.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4843..5571)
                     /locus_tag="ACGI8A_001979"
     CDS             complement(4843..5571)
                     /locus_tag="ACGI8A_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFH0179976.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5698..6387)
                     /locus_tag="ACGI8A_001980"
     CDS             complement(5698..6387)
                     /locus_tag="ACGI8A_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0179977.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6395..6958)
                     /locus_tag="ACGI8A_001981"
     CDS             complement(6395..6958)
                     /locus_tag="ACGI8A_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFH0179978.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(6960..7928)
                     /locus_tag="ACGI8A_001982"
     CDS             complement(6960..7928)
                     /locus_tag="ACGI8A_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFH0179979.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8029..8072)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8438..9067
                     /locus_tag="ACGI8A_001983"
     CDS             8438..9067
                     /locus_tag="ACGI8A_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0179980.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            9042..9446
                     /locus_tag="ACGI8A_001984"
     CDS             9042..9446
                     /locus_tag="ACGI8A_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HFH0179981.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9449..9925
                     /locus_tag="ACGI8A_001985"
     CDS             9449..9925
                     /locus_tag="ACGI8A_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFH0179982.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            10497..11162
                     /locus_tag="ACGI8A_001986"
     CDS             10497..11162
                     /locus_tag="ACGI8A_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFH0179983.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLRQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            11184..12116
                     /locus_tag="ACGI8A_001987"
     CDS             11184..12116
                     /locus_tag="ACGI8A_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFH0179984.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            12137..12778
                     /gene="pcp"
                     /locus_tag="ACGI8A_001988"
     CDS             12137..12778
                     /gene="pcp"
                     /locus_tag="ACGI8A_001988"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFH0179985.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(12898..13230)
                     /locus_tag="ACGI8A_001989"
     CDS             complement(12898..13230)
                     /locus_tag="ACGI8A_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0179986.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            13496..14116
                     /locus_tag="ACGI8A_001990"
     CDS             13496..14116
                     /locus_tag="ACGI8A_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0179987.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(14441..15265)
                     /locus_tag="ACGI8A_001991"
     CDS             complement(14441..15265)
                     /locus_tag="ACGI8A_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HFH0179988.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNT
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(15276..16679)
                     /locus_tag="ACGI8A_001992"
     CDS             complement(15276..16679)
                     /locus_tag="ACGI8A_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0179989.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDDGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(16683..17408)
                     /locus_tag="ACGI8A_001993"
     CDS             complement(16683..17408)
                     /locus_tag="ACGI8A_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0179990.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(17520..18293)
                     /locus_tag="ACGI8A_001994"
     CDS             complement(17520..18293)
                     /locus_tag="ACGI8A_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0179991.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(18299..19186)
                     /locus_tag="ACGI8A_001995"
     CDS             complement(18299..19186)
                     /locus_tag="ACGI8A_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0179992.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(19200..20222)
                     /locus_tag="ACGI8A_001996"
     CDS             complement(19200..20222)
                     /locus_tag="ACGI8A_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179993.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            20797..20973
                     /locus_tag="ACGI8A_001997"
     CDS             20797..20973
                     /locus_tag="ACGI8A_001997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0179994.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         7462 a         3376 c         2309 g         8329 t
ORIGIN      
        1 aaaagttaat caatataaat attaagccct ctttaattta tctaaatagt aaacagcatt
       61 taaagctgct atttgccctt gaccagctgc ttttatataa gaatagggtt ttccaacaca
      121 atctccagca gcaaagcatc catctatact agtcttcata ttattgtcaa ctttaatatg
      181 aatgccatca atctctattc caggaaccaa tgcacttgga gccgtactat cttttataac
      241 gaatactcca tcaacttcaa tgtggttttc tttaaaagat actttgttta caagtttatc
      301 tccatctatc tgtactggtc tgtcatgtat aacttcaata gaatcatcta ggttatctga
      361 acttctcata agattatctt ttttgtacat aggtataaaa taagtctttg aagtgagttc
      421 acttaaaaaa tttgcttctt ctttagattc ttcattatat cctataactg ctaccttttt
      481 atttctataa agtggtgcat cacatgtagc acagtagccc acacctcttc ctagaaattc
      541 ttcttcaccc ttgattggtc tagtatactc taccccagtt gcaaggatta cagtagtagc
      601 ttcatacata tcatttccag acattattgt aaagtattct cccatagcat aaatattatt
      661 tattctttta ttttcaattg aaatatccat actatctata tgtgatttaa acttgtcttt
      721 taattcatca ccacttatat cataaaaacc taaataatta tctattgaag gagctttaac
      781 aagcttatta cttaaattat cattaccaaa catgataatt gttttatttc ttatttttgc
      841 atttatagca gcagacaatc cagctggacc actgccgatt atagctatat catatctcat
      901 aatactcacc cattttttat aaatatgatt ttacttgcat ttcaattttg ttctttggca
      961 tgaatccaat taaagtttct actggttttc catctttaaa tataatcata gttggtactg
     1021 tagatacatt gaattgttga gctatttcta aactctcatc tatatcaact tttacaaatc
     1081 tagctttatc cttcatctct tcacctaatt ctgcaaatac aggtcccaac atgttacaag
     1141 gtccacacca agttgcaaag aaatcaacta caactactcc cttactacct tcaacactac
     1201 ttctaaattc agatgtattt ataacttttg ccatttttaa atcctcctat taaactttat
     1261 tatccctata taattatact atgaaattta taaatataaa tttactttta taaggtcttt
     1321 ttatatttta tactaattag tatacccatt cttcatttct aaaaacaatt ttttatattt
     1381 ttattatttt aacttttttg gtattgacaa ttcaaatgta gttccaatat ctacatcaga
     1441 agttacattt atatcaatag ataagtttaa actaatcatg cgagctattg aaagtcctat
     1501 tccagaacct tctaaatctt tatttcttac attttcactt ctaaaaaatc tatcaaatat
     1561 aagaggtatc tcttcttttt ttatgcctat accagtatca cttataagta aagttacctc
     1621 atcctctata tcttctttca attctaatgc tatctcatca cccaattttg tatacttaaa
     1681 ggcattatca atgaaaatta atataagttg tcttaattta tttttatctg ttattataac
     1741 tttattcttc agtttagaat taaacacgaa tcttttttcc tgaaactcag caatgtcaat
     1801 atagtcttct gatatttctt ccaataactt ttctaaatct atttcttcta aattaacttt
     1861 aacaatagag tcctctttag taagagacaa taaatcagtt atcatttttt taagtcttct
     1921 agtttccttc attgcagtag ctattgtttc aacttcatca tttactgtac tttctgggga
     1981 ttttaacata ctttctaatt ttgatgaaac tattgttatt ggtgttctta attcatgaga
     2041 agcatcttgt ataaacttag cttgattttt ccatgctgtt tctattggaa ttaaagcttt
     2101 tctagtgaga tatacagcca caaagtaagt aattattaca gaaattaata ttccaattat
     2161 aagaacagat gtaagctgtc ttaaagaatt catctcagaa tctatatttc taattatctg
     2221 tatttggtat ctacctacat ctacatttaa ttctctaaaa gtatatccat tttctgtgta
     2281 tttaaaaaac gaattttttt tatctagtct tctatctgga agtaactcat caaaatattc
     2341 attttgagta tagtaactta tccttccatc ttcatataca tacaccatat ctttaggatc
     2401 tttgagtctt attgggttca aaaatgaagt tcttttaaat tgagagacta tatattcaag
     2461 ttcatcattt atctcactat caattccact atatgtaagt cccctaaaat atgagtatat
     2521 aaatatagaa aagattatta aaaaactacc tactacaata atatacattt ttattaaatg
     2581 atttttagta ctggtaaata cattcttatt tatcatcgaa tatatatcct accttacgtt
     2641 tagttttaat atatttatca tatccatatt tacttagctt ttttcttaga ttacttacat
     2701 atacttccac tatttcaata gttgcatctg aatcaattcc ccatattcta tcataaatct
     2761 gctcttttaa taaaattgaa cctttattta atacaaaata ctcaagcaag ttaaactgtt
     2821 tgttttgaag ttttatttct tctttttcaa tatacactct tttttctagt gtatctaaat
     2881 ataaagattt aaattctaat ccgtttcttt cttttatctt tccattagtc cttaatatag
     2941 cgtataccct agctactaac tcttccatat aaaaaggttt tgtcaaataa tcattagctc
     3001 ctatagtaaa tgcttctact ttgtcatcta atgtttcttt tgctgtcaat ataagtacag
     3061 gtgtatctat attattaatt ctcattttct ttaggatatc aaatccattt atattaggaa
     3121 gcattaagtc taaaatgact aaatcatata tattttgatt tattaagtat aaagcttctt
     3181 ctccatcttc acacttttca gtttcaaaat gcttgctaag ttcttctact acactttcta
     3241 aaagtatctt attatcttca actattaaaa ccttcataag tatcccctac ctttatttgt
     3301 ctatcttaac tttatgagat tttaagtcaa tatccacatt ggctttttct tttatatcat
     3361 catacgtata tcccactatt tcaacacttt ttgcctttgt tgaacacaat atatctatat
     3421 ctgtctcttc tgaaggcttt aaaatcttat atgatggtac acgatttact gttaaatatt
     3481 ctccattttc attaacttct gctgtcttaa gaataatatt tcctaaatct tttgatgatg
     3541 aattttttac ttttacttga tacgtaattc cctcatttga ttcatttact tttttaagtt
     3601 ctgatgtaga tagcacctca tactgactac tattttctag cttaatatta ttatttctta
     3661 tattaatagt attttctttt aaattaacat acacaatctt accctctgcc tcatattcat
     3721 atgcataaac ttctacaccc tttacaaaag ttttatgccc acattctatg taagcaactt
     3781 tcccaggcat taaagtcatg tccaacaaag ccttactatc cgaagatata atttttttat
     3841 tctcatcata ttcattatat atcaattcaa cattggatat attaaatgtt gaattatttt
     3901 ctacctttgt tgatgtatct actttatcac cttcatctat atttataatt tctgattttc
     3961 ctatattcaa tacttttgca tttccctcat ctatttttat ctcattttta tcttctgttt
     4021 gaatatcaac tgaatcaggc tgttcatctt tttcacatgc tgtcaacata aaaataaata
     4081 ttgttaatat aatcattaga gaaattgatt tttttactat tttattcata ttaagcacct
     4141 catatagtag tattttatag tagattttta tactttttca taaattcttt caatgcctgt
     4201 gtaagtattt gatgtttttc aaaattcgaa ttactacata atctttcaaa atcattccat
     4261 atttctttat ctatacgtat tcttgttgtt tttaattcat ctatgtgttc atcatctata
     4321 tgtacatctt ttttattaga tactttttta ggctttatat tgtaatcttt tacttctaaa
     4381 taccaatcat atacttcaca aagcttatct aaatcttctt gagttatatt tatttttcta
     4441 tctttcttag gcttaatatt ttgttttggc tttgcatttt ttaaacctgt atcattaaac
     4501 tcaatttgtt caacactttt acttaaagcc ttttcattag aaatcttttt tttggtgctt
     4561 actttttttc tattacttat agtattcttt ttactttttt catactcctt agctttctct
     4621 tcttccaagt ttctattatc caaactttta ttataattta attcgtcttt taatatgtat
     4681 ttaccttttt ctgacttata tcctttttta tttaaaaaag ttcttaaagt actttgacta
     4741 atctttagat tttgagctat ctctgcaaat ttaaatcctt tgctttgaag ttctaaaaat
     4801 ttgtcaattc tgtcttcatt gttattcatt aatattcctc cgtcaacatc ttcttgtctt
     4861 tcttttcata cttcttatta tattcttctt ttctaaatct actctatttg attcaattat
     4921 tttttgtagc attttattat aagtgaaatc atctaaatta ttatttttta tatagagaaa
     4981 agtttcctct tcacatttaa taaaacaaat tgatagcaac catgccatac ccattttaac
     5041 ataatattct tcactttgta tattattaca gtattcaaat atttctttaa tatgtttatc
     5101 atcaacataa tatattaaaa acataactaa tgcaaatcta atttcccatg gattctttga
     5161 ataaacatat tttttaagta tatcatacat attttcttta tattttttca tagactttaa
     5221 atcagaacaa aatccatcgc aaactgacca attatcgatt ttaggtataa atctttttac
     5281 atagtataaa gtttcttcaa aattactatt tatattgcca attaccatac cttgtagcct
     5341 aacttcttca tagtatgtat cctgtgctac ttctaaaaat tccttccaat tcccttggct
     5401 tatttctttt gataactttc ttaaattagg aactctaata cctattatat tttctgttcc
     5461 aggtataagc tttttattaa aatctctata ttttatatct tgtatagaca tcatatattc
     5521 taaaagttca ttataatcca atttattcca ttttccccta tcaaacatca tttaattgct
     5581 ccttaattcc ttttttctta ttgtaacata aaactctaca aaaaaagctt ctaactatta
     5641 gaagcttttt caaaaacata agaaactttt caattttaaa tttaaactaa attaatgcta
     5701 atctcctaca ccaaagtctg tattattgta taaaacttct atatttggat tttcatccaa
     5761 gaatttatac ttactaacaa accatttcac taactcttca tctcttttta tactagaaga
     5821 attatttata aaaacattca ctgtcgcttt ttcaaaagta tttaggataa tctctatatc
     5881 tttatctatc atctcttttg tttgtccttt tattccaacc ataatacaag gtgaatcaaa
     5941 atattctttt acatcttgtg gagtcttaaa atttgcattt ttatttaaaa tattatttct
     6001 aaaatcattg tcaaaggttt caactccaat tttaaatacc acaggaattt caaaatactc
     6061 tctcatttcc ttaagcctat ttttgtaaga ccaatgactt tctaaaaata atttttttat
     6121 attcttctct tttataatac actttatttt ttctaaagta tccttaggta attcaaaaca
     6181 gcttccagaa tttatgactt caagaactcc atatttccca gttacgtttt ttaaaacctt
     6241 tagatttaat ttattcatct cttcttctaa attagaatta tcatctatat aatcacaaaa
     6301 agaacacttt ccccatatac aaggaaatcc tttaagcaga acaatttctc tttgattttt
     6361 atttgttatc tcactatatc tatccatttt ctatctattt tcctccagtc aatgatacct
     6421 ttaaagtttt tggaactgca tttaatccta aattcactaa aacaactgct aacagcttat
     6481 ctccataatc agtaaatact tgggttacaa acacactaaa aacatccccc agtcctaaca
     6541 ctttcaagat ttgcactata tatgatgaac ctgaagaggt catacctcca aataaaaatg
     6601 ctgaaattat tgaacttatt atagatgttg gaatggcaaa taaaagtact cctagtggag
     6661 tttttaaacc ccttaaaaaa ttctttttaa atgctattcc agcaagtaat ccggttgata
     6721 tttgaactgg tgcaaaatag agagaataca catcaaacgt aattccactt attaaacttc
     6781 caaacacacc tgtaacaaca gcgtatttag gtcctaaaag tgaagctatc attatagttc
     6841 ctattgaatc taagtataca ggtaatctta atcccatagc tataaaagcg cctattatat
     6901 ttatagaaat accaaaagct ataagtgtaa tagtttttac acatattttt cttttcacac
     6961 tatatcactc cttccacaga gtctataatg tcttcataac ctttaaaaag cctctttaaa
     7021 aacatgtaca taaatttctt ctcatcaact tcatttagaa caattgcatt aggatttttt
     7081 ttatagaaat tgaaagaatc cactatagac tgacccatag ctattccatc ttctacaact
     7141 tctacatatg attcaaatcc tttacatata cttctatcta taaagtatgc tactgctaga
     7201 gggtcattta tcacacagcc aattattccc tcctgttccc aatggaaatc aatataaaat
     7261 ctagttattt cagttatata ctttgccatc ttcttatcaa gtctatttat aaactcaata
     7321 atattaggag taagtacaat ttttctagtt acatctaaac ctaccatgtg gattttttta
     7381 gataaattct tgtaaacata atctgctcca tgtgggtcta cccaataatt aaactctgct
     7441 actggagagc aatttccatg aatcctaaat gctcctccca tagatacaaa ctcatcgaga
     7501 ttttcaaatg ctttcttatc tttaataagt gctttagcta tatttgtaag tggtgcaagt
     7561 gctattattg atactttttc atgattatat aaagtattta ttataaaatc cactgctcca
     7621 ttttttgctt tagctcctac aaccttttga taaaagtttt ctccaatacc atcttctcca
     7681 tgtgtatctt gagctgttac aagttttctt tttagtggtg cttcttctcc tatatatact
     7741 ggaatattta gtgaagaaca catttgaagt gtttttagtg cattttctgc acctatattt
     7801 gctgggacat ttccacaaca tgtggtaatt ccaattactt ctagctctgg tgagtttaaa
     7861 gccagaagaa ttgccaaaga atcatcaatt cctgggtcac aatcaattat tacttttctc
     7921 ttttccattt tttatcctcc ttgatttgtg gtcgaggaga actacacatc ttttttccac
     7981 tttcaaaaag gtgtgtctaa gggtattaat tatatactgc cactatataa ttccttagtt
     8041 ttttatactt ggagttctcg aacaagtaca gatgtaaaat catattttta ggctacttac
     8101 tataagactc ttaaaaacta tagaccgtta aaaaattttt aaacatctga tttaatagct
     8161 ataaaataaa ttttatagca taaaagaatt tattatattt taatgtatct tattaaagtt
     8221 tatttagaat tatgaaccaa ctttaataag atgtggcgca tttaaaccga ttattattat
     8281 acaataatat cttaaatatt acaattaata acattaattt attatccata aaaatatatt
     8341 aacttatatt atttaataat ttattgacag tattaaaatt aaattataca ataacagtgt
     8401 aaattaactt aacaccgtta agcaaagagg tgattatatg agtatcaaag aaagacgtaa
     8461 aaatgaaaaa gaagaaatga agaaaaagat tatggatgct agcatcgaaa ttataaacca
     8521 acatggatat gaaaatttat caataagaaa aattgctaca aaaatagaat attctccaac
     8581 tacaatttat ctttactaca aagacaaagc agagattatc agcgatatga ccaataaact
     8641 ttataacaca gtagaaagta atgccattga cattatgaat aattgctcat cactaccaat
     8701 agataaacaa atcatagaaa ttatgactag ctttataaaa actctttcta gtgaaccaga
     8761 gatggcaaaa tctatcatgc atagtagaat gaatattata tttgcatctg aaaatacaaa
     8821 caatacgcct agcaataatg gtataaaaat gcttgataaa ttcctttcca ttggaattga
     8881 acaaggaata ttcaaaaaaa atatagataa aagctcatgg atgcttataa gtgcattgtt
     8941 aggattcgtt ttatgtgttg ttgaaaataa actatattct ttacataatt tttctcaact
     9001 tatagaaaat ttcttagata ttttagtggg aggactttac aatgaaaatt cgcaacaagg
     9061 ctactaataa aaagaaaaaa tatgtaaaaa tatctattgc tctattcttt gtatttatat
     9121 gcattattat tgtaattgta agcaacttaa agccagaaaa cttggtagta aaagatatag
     9181 atattaataa tgttaaaaat ggcatttata ctggaagtgc tgataataac ttggtaaaag
     9241 ctactgtgtc tgttgaagtt aataatggta aaatccaaaa tattaatatc ttaaaacacg
     9301 accatctttt aggtaaacct gctgaaaaaa ttacaacaag tattattaaa caacagtctt
     9361 tagatgtgga tgctataact tctgcaactt atagcagtaa tactatacga aaagctgtag
     9421 aaaatgcttt aagaaaggga gaataattat gaatactatt attatatatt caagtaaata
     9481 tggttgtaca aaagactgtg caaatatact aaaaaataaa ctatcagata atgtcactat
     9541 tgttgatatt aataataata ataataaaat tgagttatca aaatttgata aaattattat
     9601 tggaagttct atatatgttg gttcagtttc aaaaaaaata caggtgctat gtaatgataa
     9661 tgtagagtta ttaaacaaaa aacaagttgg cattttcctt tgctgtggct tttctgaaca
     9721 agcagataaa tatttaaaat caaattttcc atcatcctta ttagaaagtg caaatgctat
     9781 tggaattttt ggtagtgaag ctcgattaga aaaaatgaaa tttttagata aacttattat
     9841 gaaggctgtt tcaaaaggta attatgacag ttttagaata tcacaggata atatagataa
     9901 ttttttaata aatcttaata gctaaaataa agctaattat ttctatgata aattctttta
     9961 tttataagta atattgctca aaatagtctt taaactttat caaaatttta tttttcttag
    10021 tgaaatctaa tgaaactagc acttaatata tgctttatta atcataacaa atatgtttta
    10081 ttaatcataa caaaaagaga gtgtctaatg aactaaaaaa ttcattagac actctctttt
    10141 tgtatgaaat caaatatatt tttatcattt caacagagaa aaatacactt gtctctatgt
    10201 ttggcacatt ctctttttat aatataatgt ttaatttaaa actactattt caaatattga
    10261 catatactta aactatagac ttacaaaatc aattcatatt agcttaaaat ataatctttg
    10321 ttattcaaat tcctatacac aaccattatt tactttgata ctttaaaaca tcttccctat
    10381 tttttattaa attttataat aatagtccta ttaacgcaaa tactataaaa agagtttaac
    10441 tttatccatt aaataaattt aaattaaaac atatagaaat ggaaggagaa caatttatga
    10501 taaaactaat cggtatattg atagttgtaa ttggatttat acttaaaatt gacacactat
    10561 ttactgtatt attagcagga gtggcaacag gaattgttgc aggattagat tttaatcaaa
    10621 tttttacaat tttaggagat tcttttgtct caaacagagg tgtatcatta ttcatattaa
    10681 cattgccagt aatcggtgta ttagaaagat atggtttaag acaaagagct gtttctttaa
    10741 tagaaaaatt aaaaagactt acaacaggaa aagtacttac tatatacatg atagctagac
    10801 aaatagctgg tgctctatca ataagaatga gtggacatcc tcaatttgta agaccacttg
    10861 ttaatcctat ggcacaagct gctggtttaa gtaatagtga tgaactaaaa gaatcggatg
    10921 aagaagccat aaaggcatta tctgctgcat cagaaaacta tggaaatttt tatggtcaaa
    10981 acttatttgc aggtagtagt ggagtattac ttatagcatc tactctgact caatttggat
    11041 acaatgtaac tggacttaat atcgtaaagg ctagtactat tatggcagta atagcacttg
    11101 tagtggctac aatccagttt accttatatg ataaaaaatt aaacaaaagt cataaaaaat
    11161 agttttattt aggaggaata aaaatgcaaa attttataaa catatcattg gaaatctttt
    11221 acgcactaat gggattttta atgattgtta tagcatataa gtcttttact accataaata
    11281 acaataagaa atatggtaca tcattatttt ggatacttat atcactgcca ttcatatttg
    11341 gtagattgat tccagccaat ataataggaa taatattaat attatctagt ttattaacac
    11401 tttctaaaca agttgtattt gcaaagtatg aagaaccaga tgaaaatttt ggaaaagaac
    11461 aagcagacaa acttaaaaat aaaatattta taccatcatt aatattagca tttgctgcag
    11521 tagtagtagc tatgagtctt tctaattttg ataattcttc tcagtttgca ataggtgtgg
    11581 gttcaataat tgcattaata tcagccctta taataactaa ggcaaaacct gctacatcag
    11641 ttcaagatgg ctcaagatta cttcaacaaa tgggacctgc tagtatgtta ccacaacttt
    11701 tagttgcatt gggagcttta tttactcaag ccggagtcgg tgaagttatt tcaaccatga
    11761 tatctggtgt agtccctgct gatagcagat tatttggtgt tatagcttat gtattaggta
    11821 tggttatatt taccatgata atgggaaatg catttgccgc attttcagtc ataacggcag
    11881 gaattggtat tccttttgta ttgtctcaag gtggaaatcc tgctataatt ggagcattag
    11941 cattaacagc aggatattgt ggaactctac ttactccaat ggctgctaat tttaatatag
    12001 ttccagcagc cttgctagaa tgtaaaaatg attatattgt tataaaatat caagctccag
    12061 ttgctttggt attaattata gcccacatat tagtaatgta ctttttagga ttttaatata
    12121 attaggaggt aaaataatga aaattttgtt aacaggattt gacccatttg gcggagaacc
    12181 aataaaccca gctcaagaag cagttgaaag ggtcaataat aatataaacg gtgcagaaat
    12241 cataaagata actataccaa cagttatgac aaaatctgtt gaagctatag ataaagctat
    12301 tcaagagcac aatccagata tagtaatatc agttggacaa gctggaggta gatttgacat
    12361 tacacctgag agagttgcta taaatataga tgattttaga ataaaagata atgagggtaa
    12421 ccaggttata gatactatta taaaagaaga tggtgaacct gcatattttt caaaattgcc
    12481 tgtaaaagct atggtaaaac atatgaatga aaataaaatt ccagctagtg tttcaaacac
    12541 tgcaggaaca tttgtttgca atcatgtgat gtatggaatt ctttatatga tagacaagaa
    12601 atatcctaat ataaggggtg gatttataca catcccatat acaacttcac aagtaataga
    12661 taaaaaaaat acaccattca tgtctttaga agaaatagta aaaggcttag aattagctat
    12721 agaagcttgt attatatata aagaagatgt aaaagaaatt ggtggtgaaa tttcataata
    12781 aattttttag tatttttata aattaaaaat actaactatt aatttaaata tagactgtga
    12841 gtagattatt caaacaattt tactcacagt ctttttatgg tatctacaga tacaaaatta
    12901 aggtctattc ctctctcccc actcacacat tgcatctaaa attggaatca gagattttcc
    12961 cctttctgat agactatatt ctaccttagg aggtatttga ggatattcag tacgaataat
    13021 caagttatct gcttctaatt cttttaatga taaactcaaa gttttatagg agatagtatt
    13081 tatataacgt tttaatgcat tatatcttac aacttcaaat tctacaagac agtaaagaat
    13141 tatcatctta tatttaccac taatcagtga aagtgtataa ctaaatcctg tttcacttaa
    13201 atctgtattt gatatacagt ctatactcat atattacact ctccttttgg ttagtattga
    13261 acaaaaatgt gcgtacttgt ttttagaata atttataata aaattatagt gtaagaataa
    13321 ttgaaagtca attttataat aggaggtgta tttactaaaa aaaaagttat tttaaatagg
    13381 attctaagat tagacatttc tatataaatt gataatattc atgaaaatcc tattttataa
    13441 aaaataatgt ctcttagaaa atctattaaa taaaattcat tggaggaaat ttataatgaa
    13501 agttttattg ataaacggta gcccaaacca atatggatgt acttatacag cactaaatga
    13561 gattactaaa gtattgagta aacacgatat taaaacagaa atactgtatt taggtaaaga
    13621 aacaatccca ggatgtatta gttgcgcatc ctgttttgaa actggaaaat gtattcgaaa
    13681 tgataaagtt aatgaattga ttgaagatct ggataatatt gatggtatta ttattggttc
    13741 tcctgtttac ttttctagtg caactggtca attaacttct tttcttgacc gtctattttt
    13801 catagctggg agtcgcatgg ctacaaaatt aggtgcttca gttgtgtcat gtagacgtgg
    13861 tggtgcaagt gcaacatttg accaactaaa taaatatttt tcaatcagca atatgcctat
    13921 tgtatcatca cagtattgga accaggtaca tggctttact ccagaggatg ttatgaagga
    13981 tgaagaagga ttacagacta tgcgaactct aggagaaaac atggcatggc ttcttaagtg
    14041 tatatcagca ggtaaaaaag ctggaattaa agaaccacaa tatgaagaaa caattatgac
    14101 taattttata aaataaataa aaaatgtact aatattaatt aaaacaaaac actaatttat
    14161 aaataaaata agcataaatg taggataaaa agcactaaaa cagaggtatt gaattttata
    14221 tcaaacctct gttttattat tacattaata accaaaccat gtataacatc atatatttct
    14281 aaataaatta taatattata ttctaatgtt attgaaatta caataactga atttctgctc
    14341 ataaatttaa atattctaca aatctcatct aaataacaat tttaatagac ctaaaacaat
    14401 cctcatactg ttcattttgt attaaaaata tcacttacaa tcaaaaacca tttattaagt
    14461 atattcctaa gatatacaat atatatacat ggattggata gtataaataa aataaatact
    14521 tcatactctt ccctttttct ccattgtaaa gtaacattaa aggtagagaa gcaatcatca
    14581 tccattggta attttgtaaa atagaatttt ttaaactata atctccattt gacattgttg
    14641 taaatataaa tatacacatt aaaacataac aaatactcaa actgaacttt ttacctctac
    14701 acaaataaaa aataatccca agtataatcc aaattggtcc accttctact aaaaatggta
    14761 ctggtaccaa aatcataaat aaagcaataa ctctgttagt gactttaaaa agtaaagcta
    14821 ctgtaaatat tcctaaaatt ataggaatga ttatcatcaa taatcctata ataaaatatt
    14881 taatttcttt ctcttttcta aatttcttta tgaactctat tgcttgtaaa taaatcgcta
    14941 ttaaaaataa tgttgaaaaa gtgttattta taattatagc tccttctgga tgtgaaaata
    15001 cattattaat tatttgattt cctatagcca taataacaga ccaaatatac aatctactta
    15061 tatatttttt tctgttacta gtgtgataaa atccttcaac agccatgaat ataaacagtg
    15121 gtgctgatat tctgcctacc caatgaaacc aaattggtat ttgcattgtt tgtggcataa
    15181 atgctgctat atggtcaaat gtcataaata ttaatgctaa aatcttaata gtaaacccac
    15241 ttataccttt tttaaaaaaa tccatatcat atctcctaat tttgttttat ctctataata
    15301 atttttgttc ctattccaat ttcactactt acataaattt tacctttatg aatctctatt
    15361 atttctttag aaatagccat accaagacct gaacctttat gcatctcacc tgtattagta
    15421 cctctataat attttttaaa aatatgtttc aaatcacttt tgcttattcc aattccatca
    15481 tcttttatta taatttctat attatctttt tttacaattt ctacacttat caaagttcct
    15541 tcgctattat gaactattga attaaatata agattggtta tagcccttct gaataagatt
    15601 gaatctacat atacttcaat taaatcttca ttactttcaa attcaatatt cctattttta
    15661 tatctattat catttaaaat atcaattatt atatctctga ctaaactgac taaatttatt
    15721 tttttcttgt ccagaactat agtattattt tttaatcgag tagataaatt taaatcatca
    15781 actaaatctt ttatatactt agacttatta tatataattt ctgtatactc ttgtatttca
    15841 tcttcttcaa actcataatc tttgtcatta attatctctg catacccttg tatagatgct
    15901 agaggtgtct ttatatcatg tgttatattt gccaaccatt cctctctcaa ctcttcattt
    15961 tcttttctct caacttcatt tactcttaaa gtatcagcta acatatttat atttttaaaa
    16021 acttcttcat atatacctct gtccttaaga tataaactat aatttccatt agccatagtc
    16081 tcaatactcc atagtacgct tgaaattggt tttgtaagtt ttctactaaa taaatatcct
    16141 acacctaaag ctaccacgct atcaattact agtgttataa tgataacttt atttaaagtt
    16201 tttttaatgt tattttgaga ataggttaaa attattcttg atatatctct actaggtata
    16261 cctactatta tgtttagatg ttttttgttt aaatattttt ctaatacaaa attaacatac
    16321 tttgtttcta tatacttgta attattcacc atttgaaagg gtgtatattt tttgggagta
    16381 tcctttggca catttactcc ataaacttcc tctcctaaat catttaaaac ctgtatccag
    16441 gaatttgatt tttctaattt ttcagctcca atcttagata gtttaaattc tccatctttt
    16501 tcatataaat ctttttcaaa agaacgtgca aattcttcag gattatttcc agaatctaca
    16561 actttaaaaa aactattagt tgaaatcaca tacaatatag atatgatatt tataattact
    16621 actgatatag ctacaaacac aattgtaaat ataaaatttc tagtaatctt ccacttcata
    16681 ttctaatctt cctttactgc taatttatat ccaagacctt ttattgtcag aatatattca
    16741 ggctttgaag gattatcctc taatttttcc ctaatttttc ttatatgaac cattatagta
    16801 ttgtcaaatc caacataatc ttctccccac actttatccc atagtttttc tttacttata
    16861 atttgatttt gattatgtgc aaataaagat aggattttta gttcctttgc agacagtcct
    16921 atagattttc catcttttat aagttctgcc ctactttcat ttaatatata tgggccaaat
    16981 gatataattt tttcttcatt attatttaga tttttacttt cgttttgagc atcactaaaa
    17041 taacttaatc ttctcaaatg cgctttaact ctaaaagcca cttccttagg gctaaaaggt
    17101 tttgtaacat aatcatctcc tccaatcgct agtccaagta acttatctaa ttcttcggtt
    17161 ttagctgata aaaatattat tggagaattg gaggtttttc ttatttcttt acaaatatca
    17221 taaccttcgc catctggtaa cataatatcc aatacaatca aatcaggatt aatactttta
    17281 aataattcta atccttcttt tttagttttt gcagtatata tattattaag gccctctttt
    17341 tttaggacag tttctaaaag cttcaatatt tctacttcat catctattat taaaacacga
    17401 ttttccataa acaacctcct gtaataattt acatatatat tagcatatat tttatctaaa
    17461 attcatttat cttattttta attaaactaa aatataaata aaattactat tatttttagt
    17521 taaaaccttt tgcttgatgt aaaattttca tatgttattt tagttaacac tatcattaat
    17581 ataattgaga ttactataga aaaaactatc aaaatacttg gtacattata cttaaccatt
    17641 tgttggctca tcataatcca atttggagtc actgacataa attttatagc tttatctggt
    17701 ataaaaccta cactaaagta tggaattgcc attatcaatc cacctagtat aaatggaatt
    17761 acagccgatt taaatataga tgaaagtgtt aagcctattt gtgcaaatat aaaagaagca
    17821 aaaatagtag tcaatataac aattatatac atttgtatta ctgtaagatt taaattacta
    17881 tttccaaagt accataagtt atgaatcgat tcatttaaat ttttaaaatc aaaaaaacta
    17941 aaacctctaa ttcctactaa caatataaaa gaaatattaa taatagataa aattaatcca
    18001 ttagctacta gttttgataa tactactgat ttttttccct ttttagttgt atttattaaa
    18061 cctgtcatat tagaatcata ttctttaatg taatttccag aagttattat aaacattatc
    18121 aaacctatta gaggtattga gccttctatc ttgtaaaata tatctgcata agtatctaaa
    18181 acatcaaact ttctaatcag aaaaaatcca aaaaatatta aaaacagcca aattatccaa
    18241 acactctttg attttaatat ttttatcatt tcagctttaa ctaaatacat catttctact
    18301 acacctccct tgcatcttct aaatcaaaat aaaacatgta tacatcttcg aattttgctt
    18361 gaactggttg tatattatcc tcgcttggaa tatcctctga aacaattcta agtactactg
    18421 aatcaatatc actttgaata cttacaacct tatatttaga ttgtatatca tcaacttcac
    18481 attcattttt aacttgaaca ttatatactt tattttccat gtgacttaat atgtctttgt
    18541 gtgttccttt cattaggagt ttgccatctt taatcattat tgtttccttt gcaactgatt
    18601 ctatatcgga aattatatga gtagaaagta ttattatctt atctctagac atttttgcta
    18661 ttaaattcct aaatctagct ctttcttgtg ggtcaagtcc tgctgttggc tcatcaagga
    18721 ctattatctt agggtcgttt agtaaaactt gtgctatccc aactctcctc ttcatacctc
    18781 ctgaaaattt tcctatagac ttatttctca catcataaag acctactaaa tctaataatt
    18841 ctcctatctt actatttaaa tattttcccc ttaaaccttt taaagctcca atatactgta
    18901 aaaattcttt tgctgtaaaa tttttataaa ctccaaattc ttgtggcaaa tatcctaaaa
    18961 tatttctata atcagcatcc attttaaata tatcctcacc attataaagt atctgacctt
    19021 catcaggttt tatcaacgtt gttatttgtt tcattaatgt agattttcct gctccatttg
    19081 gtcctagaag accataaact ccattttcca gagttactgt aatcttgtcg tttgctactt
    19141 ttttaccata tatctttgaa agattttcta tttctaattt attcatctta actccccttt
    19201 tatctactta tataatctga ttttattact aaaaaacata atgacatagc acctatcaat
    19261 attgccatca aaagtcctat tgctatattg acaatgtcta tattcacttt aaatatcata
    19321 atatcaccta gataatttcc tattatttta ttaaaaccaa atatcaaatc ataataattt
    19381 acatttgata caactgaaag taatttttta ggtaatatat ggattaccga cattatctta
    19441 gttatagata taataaatat aaaaaaactt attgaagata ttgaagactt acttataaaa
    19501 gacccaaata tggctatcat ggcccatcct tcaaacatca aaagctctat gcctatattt
    19561 aatattacat actttgttat acttaaatta gttttcaatg caactacatt gtctactatt
    19621 ctaaaagatt ctaagtctcc attaacaggt actccttgtt gcatacaagt tataataaat
    19681 attcccatta aataaacaga ataaattact aatggaacta ataaagcaac aaatatttta
    19741 gactttaatg cttttttctt ctcttttgat gatagtatga tatcttttac cgaagtaacg
    19801 acttcatcta cataaatatt ggatataatt atagatataa tgattaacat tcctacattt
    19861 ataagtgaat ttgtgactct atagttaaat actttccaaa aactaatatc cttatattca
    19921 ttcacactta gattatcaag ttttttcttt gacattaatt caattttttt agaaaattca
    19981 cctgtatctg tttgatttga aaactgcttt aaaacattaa tcttattttg aaatttctca
    20041 ttacctacgt cgataccact tctcgtatct tctatacgtc ctttttcttt atctatataa
    20101 taactttctg tttctaaaac aggcgtaata aagatactaa tcattagtat taacactaat
    20161 atccctagag atataaacat actcttagat ttagccaatt tttttatttc ccaatttacc
    20221 aatatatttc tcctcctaaa tcattttaat tacacatata tattaaaact taaatcttat
    20281 ataaatataa tactaacctt aaataaagct taaattattt tataatttat aggtgtgttt
    20341 attattggag ttacatttat tatttttctg ggaaattata cattttcaat tatttttatt
    20401 atttttgata tttttattga ttggaaattt tattgttgtt caaattgtag ttattcttta
    20461 aatttaaaga tggagttttt taagaataga acctactgtc cttattatgg aaagattatt
    20521 aaatagttgc atataacttt atttatcttg aatcttattt ttaaaaacaa ttataatttt
    20581 agaatatttt ctattctcaa acaagcatgt tgaataatgg agcatcttct agtattatat
    20641 acttgtagaa taaaactaaa ttggtaaaac tgggaatatt taaccttaag gagttaagtt
    20701 atagaaacta gtactaaaaa attttataaa ttatgttaat tgactaatag aatgcgactt
    20761 tttattttta ctaaattttt tagggggatg gataatatgt ttattggaga aatagacaaa
    20821 tgtacacata tactaactgc ttacatcagt agttcatatg attattgtaa ttttttagat
    20881 acacagttag atgattttat atcagaatat ggagaaacgg tagttgaaac ctgcttacat
    20941 caagtcttat tattagtaag tagatacaat taaaactaaa tttttctaga aaaacctttt
    21001 tagatttatc tactctaata aatatataat tttttgtact ttataagtaa ctataacttc
    21061 tgctaaagta aagatgtatt gggagtacat ctttactttc ccattataga agttaaaagc
    21121 atacaccata aaattaatca tccaatacaa attatatatt tcctatgtct aacttaattt
    21181 acaagaatca ttctatatgc atattctgca atccactaaa catggctcta aacctttact
    21241 attttataca ttctctataa atcatacaaa tcatatataa aatcccccta atatgtttaa
    21301 accacctata aaataacacc aatatttaga atattcaata tcaactgcat aactggtcaa
    21361 taaaaacgta taactagaca aatttcaata tttatataaa taatacaaga aaaacaagca
    21421 ttttatttat ataagttttt tattttttgt ttatattaaa aaactatatt gattaa
//
LOCUS       DAYATN010000056        21414 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.056, whole genome shotgun sequence.
ACCESSION   DAYATN010000056 DAYATN010000000
VERSION     DAYATN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.056"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            166..1176
                     /locus_tag="ACGI8A_001998"
     CDS             166..1176
                     /locus_tag="ACGI8A_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFH0179995.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            1312..1800
                     /locus_tag="ACGI8A_001999"
     CDS             1312..1800
                     /locus_tag="ACGI8A_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFH0179996.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1976..3904
                     /locus_tag="ACGI8A_002000"
     CDS             1976..3904
                     /locus_tag="ACGI8A_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0179997.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            4081..4491
                     /locus_tag="ACGI8A_002001"
     CDS             4081..4491
                     /locus_tag="ACGI8A_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFH0179998.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4513..4815
                     /locus_tag="ACGI8A_002002"
     CDS             4513..4815
                     /locus_tag="ACGI8A_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HFH0179999.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(5032..5724)
                     /locus_tag="ACGI8A_002003"
     CDS             complement(5032..5724)
                     /locus_tag="ACGI8A_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFH0180000.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5750..6937)
                     /locus_tag="ACGI8A_002004"
     CDS             complement(5750..6937)
                     /locus_tag="ACGI8A_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0180001.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            7273..9150
                     /locus_tag="ACGI8A_002005"
     CDS             7273..9150
                     /locus_tag="ACGI8A_002005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFH0180002.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            9507..10913
                     /locus_tag="ACGI8A_002006"
     CDS             9507..10913
                     /locus_tag="ACGI8A_002006"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFH0180003.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            10941..11789
                     /locus_tag="ACGI8A_002007"
     CDS             10941..11789
                     /locus_tag="ACGI8A_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFH0180004.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            12450..13142
                     /locus_tag="ACGI8A_002008"
     CDS             12450..13142
                     /locus_tag="ACGI8A_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0180005.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            13139..14074
                     /locus_tag="ACGI8A_002009"
     CDS             13139..14074
                     /locus_tag="ACGI8A_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0180006.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            14222..15145
                     /locus_tag="ACGI8A_002010"
     CDS             14222..15145
                     /locus_tag="ACGI8A_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180007.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            15129..15899
                     /locus_tag="ACGI8A_002011"
     CDS             15129..15899
                     /locus_tag="ACGI8A_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180008.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            15909..16691
                     /locus_tag="ACGI8A_002012"
     CDS             15909..16691
                     /locus_tag="ACGI8A_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180009.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            17068..17604
                     /gene="rbr"
                     /locus_tag="ACGI8A_002013"
     CDS             17068..17604
                     /gene="rbr"
                     /locus_tag="ACGI8A_002013"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HFH0180010.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            17636..18025
                     /locus_tag="ACGI8A_002014"
     CDS             17636..18025
                     /locus_tag="ACGI8A_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HFH0180011.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            18062..18448
                     /locus_tag="ACGI8A_002015"
     CDS             18062..18448
                     /locus_tag="ACGI8A_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HFH0180012.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            18575..20017
                     /locus_tag="ACGI8A_002016"
     CDS             18575..20017
                     /locus_tag="ACGI8A_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HFH0180013.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            20314..21129
                     /locus_tag="ACGI8A_002017"
     CDS             20314..21129
                     /locus_tag="ACGI8A_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0180014.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         7909 a         2488 c         3918 g         7099 t
ORIGIN      
        1 ttttgggtat tgtatactgg aattagagta attaatttat tttttaacat acaaatgatt
       61 ttatgaaaaa aaacaagact caaaaatttt tatattcttt gatagaataa aattgagagt
      121 aaatttattg aattttataa aatttataag gggtgagtct aagatatgca tgaagataaa
      181 aatgatatat ttgaatcaat agatactgag gattttgata ataaaacaga agaagaatgt
      241 ttagagctag taagaaaaaa tggattgaat ttaatataca taaaaaatca aacagaagag
      301 atatgtctag aggctgttag acaaaattgt aatgcaataa aatgtgttca gaatcagaca
      361 gaaaaaatat gtatagaagc agttaaacaa gattggagaa tgttagaatt tgtggaagag
      421 ccaacagaag aaatttgcat gttggctatt aaccaagatg gaacagcatt aaaatatata
      481 gaaaatcaga cagaggagtt atgcttaaga gccgttgaaa aaaatggtgc agctttggag
      541 tatgtaaaag aacaaacaga agaaatatgt atagaagcag ttagaaacag cgaatttgga
      601 ttggcaagag tgaaaaatca aacagaaaaa atatgtatgg aagcagttaa gcattgtagc
      661 tataatctaa aatgggtaga aaatcaaaca gaagaaatat gtatggaagc agtgagacaa
      721 aatgggcttg atttgaaatt tgtaaaaaat caaacagaaa caatctgttt aagagcagta
      781 agacaaaatg gaatggcatt agaatttgta aaggaacaga ctgttggtat atgcttaaag
      841 gcagtaagac aatatggaat ggcattaaaa tttgtaaagg aacaaacaga agaaatttgt
      901 acagaagcta taaaacaaga taaaagagca cttagttttg taaaaggaga taaggaaaaa
      961 tataaggctc tatatgataa taatgaacca tttgcaaaga gatatgttag aaatgtaata
     1021 gaaaaagaaa acagagcatt gattaaaaaa ggagaagaaa atgcaaaaat caaaattgca
     1081 ggaaaacttt atgatagagg catgtctttt gaagatatat cagatatagt tgagattgaa
     1141 ataagcaagt taaaagtgag tttaggtgtt ttttaaagta ataaatgtag gtgtttttta
     1201 tgggaaatag ttaataaaaa aataatcgac tagctctttg taggacagca aaattagtga
     1261 tataatttga tgtataataa attattttta ttagataagg gggaattaaa gttgaagatt
     1321 acagtattat accaaacaag aagtggaaac acagaaaggg tcgctaagct gatagaagag
     1381 ggcgctaaaa aggtggatgg agtagatgta aagcttatga gacttgataa tattgattta
     1441 gattatttaa atgaaagtaa agcagtaata tttggaacac ctacttattt agcaaataca
     1501 acttgggaag taaaaaaatg gtttgatgaa gattcaaaaa aagttaattt agctggaaaa
     1561 ttaggagctg tttttgctac atgtgattat atatgtggag gtccagatgt tgcgatactt
     1621 actatagtag gacatttgat ggttaaaggg atgcttgtgt attctggagg tggttcttta
     1681 ggaaagccat ttatacattt aggacatgta cattcaattg aaggtccaga gttacaagat
     1741 gagaaggcta taatatttgg tgaaagaatt gctactaagg caaaagaact atttgcttaa
     1801 tttctatatg catagtaatg attagagtaa atttagggtt ttaatggaga tttgaagtaa
     1861 aagttataaa ataaaaaatt ttccataaaa ttactcaaga tgaattataa tataattata
     1921 aataaattat attattaaaa tatttaaaat caaacgtcaa tagcgaggga agtttatggt
     1981 atggcatata gaatatgaga ttttttctgc aattatagtt atttttttaa tggtatactt
     2041 ttttagaagt aaatttatac ctactttaca aaacaaaata tattgtgcac ttttaatatt
     2101 ctcattttta tttataattt caaatatttt aggttcattt tgtctaaata atatagacaa
     2161 aattccaatt tttattactt ttttattgaa tcagatatac ttattgttat tgccaatacc
     2221 agcagcactt gtatcatttt atgttatggc tattatttat caagatataa ggtatatgaa
     2281 aaaaagatta ttatttttat ctataccact tataatttgt ataatcttat ctattacaaa
     2341 tccttttacg catattctat ttggattgtc aaaagaaaca ggttatatac gaggttgtgg
     2401 atatgttgca acttttgcat cttttttctt ttatgcaata tattcagcat ttttatcttt
     2461 taaatataga aaagcaatgc atgaatcaaa aatatgggct attagaggtt ttcttatagt
     2521 ttcaattgtg gcaattatct tacaggcaat ttttttacag tatcttctta caggaactgc
     2581 atgtgcatgt agtttacttt tgacatatct ttctatgcag aatcgaggtc ttattattga
     2641 tgaccttact ggtgtactca atagacaatc ttttattcaa gagctagatt tgaatatcaa
     2701 tactgaacaa tcaggattta ttattacaat tgcgcttgat gattttaagt ttatgaatga
     2761 aacttttgga acaaaaaatg gagatattgc actaaaagaa gttggaaaat atttgattca
     2821 gatacatgat gtaaaccatg tatatagaat agggggagat atattttcta ttgttgtcaa
     2881 tagtcaatta ggtatagaac cagatgatat tatcagaaag atagaagaaa gatttaaaaa
     2941 gccttggcta gtagaagaga taagttttaa tttagcaact agtattgctg ttgtttatta
     3001 cccagaaaat gcagaaacga cagaagatgt aattgttgct atagatttct ctatacatga
     3061 agcaaaacgc tctgagtcaa ggcgagttgt ttatgcagat gcgtctataa gtgaaaagat
     3121 aaagaagaaa catgttatta aaaattgttt aaaagaagca ttaaaaaatg atgggtttga
     3181 agtgtactac caacctatat tttctaatag caggggcagg tttacttcag cagaggcatt
     3241 acttaggcta gaacacaaag agttaggttt tatacctcct gatgaattta ttccaattgc
     3301 agaacaaaca ggattgatta actcaattgg attagtagtc tttgagaagg tatgtagatt
     3361 tatagctagt gatgagtttg aagatttaaa attggataat atagctgtga atctttctgt
     3421 agtacaatgt atgcaaaaaa acttagctga tgatttactt tatcttatga ataaatataa
     3481 tgtatcacca actaaattta aacttgaaat tactgagact gtggctatgg gctcttttaa
     3541 tataattaaa gaaacaatgg aaagattaat tgatttaggt gttaagtttg cacttgatga
     3601 ttttggaatt gcttattctg gagttactaa tatgctttct ttaccatttt cagtaattaa
     3661 gttagataag agtttgattt ggtctatgaa tgaagatagt aggcataaac ttacagttga
     3721 gacgattata gcacttataa ataaattgaa tatgaaggct gtagcagaag gtgttgaaac
     3781 cattaagtgt gctgaagatt taatagctat ggagtgtgaa tatttgcaag ggtattattt
     3841 ttctaaacca gttccagaag atgtttttaa aaatttgttg aaagaaaata cttttacaag
     3901 ttaacaccct ttattttttt aattacaact ttgttcattg acactgccag cgatatatta
     3961 taaaattaac acaataataa atattaaatt tattatacat attattaata ttaattaatg
     4021 gagtaaagat ttattatata ttttatttaa ttatcctaaa gtgagaaagg aagatgtgac
     4081 atgaataaaa agaaaatatt agtgccaatt gatgggacag aaagaagtat gcattctttg
     4141 gaatttataa aaggaatatt taaaaaagat gaagttgaag tagaaataat gaatgtaaaa
     4201 gaactagtat ttattgatgg catatccctg gctgaagaga taaaaaattc tgaaaattta
     4261 ggaagaagaa tcttggataa agcagcagag ataatgggag agtatgatgt taaagtacat
     4321 tttacttttg gatatccagg agatgagata attagaaaag caaaagaaga taatgtagat
     4381 tttatagtta tgacaaaatc tactaaaaaa ggtcttacaa gaatgatagg ttcagtaaca
     4441 gcaagtgtag ttaaacaagc aaaatgtata gtgatgattg taccggagta gaattagaaa
     4501 ggtatttgaa atatgaataa aaagatagta gcagtttgtg aaagtgaaag aagtttaaat
     4561 tctcttaaga cagctgcaaa tgatttagga tttgatttag tttatgaaat tcaagaaaat
     4621 gataatataa aaaatgaact ttctatttat gatatagaag atgcatccat agtgttattt
     4681 gttacaagtg attctataga aagcatagag aagattgata gatttataga tagagaattt
     4741 tatgaagttg atccaaagta tataatagag gatgcaaaat ctatagtaag tgaaatttta
     4801 atagacttaa attaatcgtg ggtgtcgcac taagaataga gcgacgcctt tttatatatt
     4861 ttacacaaga aataaagagg ttgtatcaaa atatagataa gagtcttttt cattgttgaa
     4921 atgatgaaaa tatatttgat ttcatacaaa aaagagtacc tcataactaa aaagttcact
     4981 ttgagacact ccatttttat atgttagttg catttaaggt aaaatagaag tttaatcatg
     5041 ttgaaataac tgaattacat cttctaaatg attgtacatt gatgtataac ctaagtaaga
     5101 gtctttacta tcaaatgcaa tcattatctt gttatggtgg ctaatcatat ccttgagaga
     5161 atttctatat ttaaaaccaa agtattggtt agaagaaact gttttaggta ttaactcata
     5221 atattttata aaaattggat tatgagaagc taaaattatc tctttgtgaa attgcaagtc
     5281 agcatcataa cattcttcta tcaaaccatg agaaacctta tccaattcct ctattgactt
     5341 taggtaagta tattttattt tttgaagttc tttagatgta gcatttaaaa ttgctagttt
     5401 agtagcttcg ctttcgataa gttgtcttgc tcttaaagat tctacataaa attcttgtat
     5461 gttatgattt ttatagattt caccatcatc aaataggtca acttttttaa atccttcaac
     5521 tataggactt gaataaggca cagctctaag tattccttcc ttacagagta tatttatggc
     5581 atttcttaca ggtattctac ttatgttaaa taggtcagca atctttctct cacttggtaa
     5641 tttactatta attttaaaag ttccatttag aatgtttaat ttaatataat caataatttt
     5701 ttcagtggca tttaagcttt tcataaaaac tcctaaacat ataatttttt tatgattgta
     5761 ttatatcata gttaagagtt aaatctactt ttttataata tgtataagac aggatagagg
     5821 ccaaaatagc tataatagcc aagataaagt atccatattg tattgaagta tattccgata
     5881 ttttaccaac tatcattgga aatacagaca ttcctatagc ataaattgct tgaaaaaaac
     5941 ccattgcagt agatttctga tgttttggta catttttcat agactcgcta gtaaggtaag
     6001 aaaggagtat acctgtagat atacctggta gtacttgtag tacaaatatt atattaatat
     6061 tttttattgt tggaactaaa aagcaatagg ctgaaactaa aataaaaact aaaggaatcc
     6121 aaaattttgg tccatactta gtacaaaatt tagtagaagc aaagccagaa gaagcaacag
     6181 cacacaccat gtaaaatata gaagatagac ctatgagggt agaactagaa cctaagtttt
     6241 taagaatttg agtagtaaat gacattgttg tagacatttg aataccctgt tgaattaatg
     6301 ccataaagga aaaaaataca agtcttttat ccttgcagat agataaatat tttttaattg
     6361 atattttttc aggtattttt ttagaattat gattttttat atttaaggct agaatcgttc
     6421 caataactga agacaataca ctcatgatac ataaggctgt cattcctaat ttttcataaa
     6481 ataaagtact cataacaaat ccaatcaaca ttcctagatt gtttaccaat attatcgtac
     6541 ttgtagcttt ttgttgatgt tctgtagaaa aaaagttggt atagtgaacc atgtaagaaa
     6601 tccaagttga tgatgatatt ccagataaaa tgttagctat aagaaaacca ttgcctccag
     6661 gagaaaagac tcttataaga gatgctaaac cagcaaagaa aattccagac agtataaata
     6721 ttttatgttt atttatggaa tcagcaaata ctccaacagg aagccttaat aaacattgtg
     6781 atattccata tgcacctatt attataccaa tcatatttgt agatacatta catgcactta
     6841 aatatggatt ttggaaaggt atatatacat attgtgaaaa ccagaaaaat gtaacaatag
     6901 aaagtaaaag taatttttga ttagatattt ttttcatatt atcctccatg tggtattttt
     6961 ataatgcaaa taatatacca tttagatttt ttaatcaagt actttctcta ttaaaaaaaa
     7021 tttaaatata taattattat ttacaaaaaa attatacttt atagttgaat taagaaaacg
     7081 cttatgatac tataaagtta acaaatttga atatataaca gtagacgtgt tactggtaat
     7141 gcaggcaaga tcgaatcaat ttttatagat tatctataga gtactatggg tataatctat
     7201 ttatttggtt cgattttttt gtttaattgg ccataaaaca aaaagcctat agttgaaaat
     7261 ggaggatttt ttatggatta taaaaatata gctcaagaaa ttctactaaa tgtaggagga
     7321 aaagagaatg taaatgaagt gactcattgt atgacaagac ttagatttaa agtcaaaagc
     7381 gcttctaaag tgaataagga taaattatct aaaactgaag gagtaataac tgttgttgag
     7441 agtatgggtc aaatacaagt tgtaattgga aataaagtaa aaaaagtata tgatgaggtc
     7501 ataaaaattg tacctcaatc aaataacata ggtaaaaaga atgaaagtca agaaaagcaa
     7561 ggtataatta attctatatt atcagcagta gctggtatat ttacacctac aatacctgct
     7621 atagcagggg ttggtatgat taaaggtatt ttatcagttc ttgctatgta ttatatgaat
     7681 aaaaatggag tagatataaa agaaacgcaa agctatatta tcttaaatgc tatggctgat
     7741 tctatattct attttatgcc tattatacta ggatatactg cggctaaagt atttaatgca
     7801 aataaaataa tatctatggt attaggagct acactttgtt atccaacttt tactgctttg
     7861 atggcaggtg aagaatctgt tagattttta ggattggcag taacaaaagc tacgtataca
     7921 tcatctgtta ttccaataat tattgctata tgggctcttt catacgttga aaaagtatta
     7981 gaaaagtata tacctgaggt aattaaaata ataatggttc caactttatc attggtagta
     8041 atgttacctg caacattatt tttatttgga ccaatcggaa tttatatagg aaatgtcata
     8101 aacttttctt ataagtatat ttatgaatta agcccagcat tgtgtggagc atttgtaggt
     8161 gggttatggt gtgtgttagt tatatttggt gctcatagag cattattacc aataggtata
     8221 agtgatgttg ctcaaacagg aagacaaaat ttattggcgt ttgcaggagc tgcaaacttt
     8281 tcacaagcag gggctgctct aggagtattc tttaaaacta aaaatcaagg actaaaaaca
     8341 atttctatgt ctgcaactat aacagcttta tttggtataa ctgaacctgc aatatacggt
     8401 gccaatctta gattaaaaaa acctatggta tgtgccgtaa tatgtggtgc atttggaggt
     8461 gcgataatgg gaatgggtgg agcttatggt aatgcatttg caaaccaagg tgttttaact
     8521 atacctgtat atgctgaagc aggtgcatta ggtttcttaa gttatctagg tggttgtgct
     8581 atagcatttt ttggttcagc tatttcaact tatctagttg gatttgaaga tttggaagag
     8641 tcttctaata aggaaaattc atctataaaa gtagaaacga aggatggtgt tattgatata
     8701 acttcacctg tagagggcga atgtatagaa ttgtcagaag taaaggatga tgtatttgct
     8761 tcaaaagcta tgggagaagg tatagctgtg ttacctacaa aaggagtaat aacagcccct
     8821 actgattgtg aagtagcttc tttatttcca acattacatg caataggatt aaaattagat
     8881 aatggagcag aaatgttaat acatgttgga atcaatacag tagagttaaa tggtaaatac
     8941 tttacaaaac atgttaatca aggagattta gtgaaaaaag gtgataaact tatatcgttt
     9001 gatatagata aaataaaaaa ggctggttat gatgtaacta cgccagttat agtaaacaat
     9061 acatttgatt ttgggcaagt agtttcttgt aaatctagtt atgtttcaac caatgataat
     9121 ataatttcat tagtattagc tggtaattaa gttaatttga ctagagataa gtaaattgat
     9181 ttaaaaggaa ataagtaaat caacaattaa atacatctaa aagtaagttt attgcaatga
     9241 attgttaaag aaaataatta atatggagta aatctattgc aatgaagtaa caaagaaaat
     9301 aattaatata gagtaagttt attgtaatga attgttaaag aaaagagtta atatagagta
     9361 agtttattgt aatgaattgt taaagaaaat aattaatata gggtaaatct atctcaatga
     9421 aataacaaag aaaagagtta atatagagtg aacttattgc aatgaatcaa caaagaaaat
     9481 aatcaatata aaaaggagaa atatttatga atacaggatt tccaaaagac tttttatggg
     9541 gagcatcatc ttctgcattt caagtggaag gagcttggga taaagacaat aaaggaaaaa
     9601 ccgtagccga ctataattct tttaaaaagt ctcatttaca ggcagatact aaagtcgcat
     9661 cagactttta ccataattat gaagaagata ttgaattaat gaaagaatta gggatgaaaa
     9721 catatcgttt ttcaatttca tgggcaagaa taataccaga tggtgaagga gagataaacc
     9781 agaaaggttt agaattttat aataagataa tagacaaact gatagagtgt gatattgagc
     9841 cattcgtaac tttataccat tttgaccttc catttaaatt agtagagaaa tataatggtt
     9901 gggaaagtag agaaactgtc tatgcttttg aaagatttgc taaaatatgc tttaagcatt
     9961 ttggagatag ggtaaagtat tggcaacctc ataatgaaca gaatttaatt gttagagtgg
    10021 aggagagaat taatatatat gatgaaacag attcatggaa gatagataaa atgcgtgctc
    10081 agatggatta taatctttgc ttagcacatg cattagcagt caatgcatgt catgagatga
    10141 taaaagaaag taaaattgga gcagcagtat catcatctgt aacatatcct cttacttcta
    10201 agccagaaga tgtatatgca gctagaatga atgataattt taaagtgtac tatatgcttg
    10261 atatgcatca ttatggagaa tatccagggt attatatgaa gtatcttgaa aaaagaaata
    10321 tagttcctca catggaagat ggggataaag agatacttaa aaaagctaaa atggatttta
    10381 tagctgtaaa ttattatagg acaaattgtg cagaagcttt acctgaagat tcacaacatc
    10441 catttggatt aagagaagga actgttgatt ttagtatgta tggactattt aaaatgtcaa
    10501 tgaatcctaa cttagaggct tctgagtatg gagcagcaat agacccatca ggtcttagag
    10561 ttgccttaaa tgaatattgg caaagatatc acttacctgt tataataaca gaaaatggtc
    10621 taggagctaa agatatatta gaagatggaa aaattcatga tgactatcgt atagactatt
    10681 taagaagtca tataaatgca tgtaaactag ctattgaaga tggtgtagag atgattggat
    10741 attgtccatg gtcatttaca gaccttttaa gttcttctca aggttttaat aagcgttatg
    10801 gacttgttta tataaataga actgaccatg aagttttaga tttaaaaaga atcaagaaag
    10861 atagttttta ctggtataaa gaagttatag agaacaatgg aatagtaaaa taaacataaa
    10921 tatatattga gggttttagt atgtacatta caaaggtatt aaataattcg ttgctacttg
    10981 caaaggatga taatgatgaa gaaataatat tgatggggaa aggcattggc cataattata
    11041 aggccgggtc tgagttaaat aaaaaagata tagaaaagat ttttgttctt catgatgaaa
    11101 atataaaaaa gagtattata caattggcaa cagaaataga tgcagaatat tttggtatag
    11161 ctcaaatgat aattgcatat ggaatagaaa aatataactt aaaacttatg aaccatatat
    11221 atttggcctt aactgaccac attgcctttg cagtgagacg ttttaaagaa ggtataatga
    11281 ttgaaaatca ctatttgttc gaaatcaagg aatttaatcc aaaagagtat gatataggaa
    11341 aatatgctat tactgttttt aaggaggttc ttggtttaga cctaccagaa gaagagattg
    11401 gtaatatagc aggccatttt ataaatgcac aacaagataa tccttatagt gatagaaata
    11461 agagaagtgc taaaattgta aatgctattt tgcaaatagt tcattatcac ttttctattg
    11521 tatataataa agagtcattt tattatagga gatttgtaat gcatctaaag gccttttcac
    11581 aaagatttct ggctaatgaa ccatcaagag aaaaaataga ttttatatat gagcaagttc
    11641 aaaaaaattg taaggaagag tatgaatgtg tgaaaaaggt aggagcttat atgtataagg
    11701 aatactccag agaattacca agacaagagg agttatattt gatgattcat attcataaga
    11761 ttttaggaga attatgcgaa aatagttaga atatcttaat atgctagtaa tattataaaa
    11821 aattaaaaat taactgcact ttaaatatga ataattgcat tacttaaggg ggttgtctta
    11881 aaattgattt ttttaattaa atttgaagct caactccttt tttaaacctt tatatcatta
    11941 caactttaat gtttatacga ctgtacttta gatgtatatc tactttttac atttctcaac
    12001 atgcaaacct accatttttt gcagtatgtt aattgaaata aaccattgag ttaaccctaa
    12061 atttattctg gacttatagg aatagttcca aagatacttt aaaatatttt ctggaataat
    12121 tgcttatttc acatatgttt tattgtgatg ttttgattta ataaattctt caactctgta
    12181 tatagtaaat gtattatcat gtttgtttta ttcaagcaca cttataatac gcttgatttt
    12241 ttcacaatga tgaaatatac taatgggtgt tataggtata agaatcgcat tacacttgaa
    12301 gaagtaagtg ataaatgagg tataaatcct catatgataa actactattt cattgaggaa
    12361 catattctaa gtgatgtcaa gatactacaa tatgactaaa ttcctagtga taaaaaagtc
    12421 tacgaatgag tgtagaaagg ggaactaaga tgaataaagt tttaattata gatgatgata
    12481 aagatctttg tattttgtta aaaaagagta tttcaattga acagattgaa gcaaactatt
    12541 gccattctgg gagagatgga ctggttgaac tttcaaaaca agattatcag ttagtagttc
    12601 tagatgtgat gatgccaggt atggatggtt ttgaaactat ggaacaaata cgaaaatata
    12661 gcagtattcc catcttaatg ctgacatcaa aaagcgataa ttacagcaaa gtgcatggat
    12721 tgcgagctgg tgcagatgac tatctaacca aaccatttga aattacagaa tttactgctc
    12781 gtgtactgtc tttaatccga cgttacaccc gttttaatcc aaaaaatgag gagccacagc
    12841 aactttcttt caaaggtttg ttgattgacc ttgaccagca tacagttact accaatggcg
    12901 aacctatgtt gcttccaccc aaggaatttg gtgtacttct ttactgtgca aggcatcagg
    12961 gtaagatttt gacaaaacag cagatttatg aagccgtgtg gggtgagcca tatgtatatg
    13021 atgacagcaa tatcatggct attatcagtc gcctaagaaa aaaaattgaa ctagacccat
    13081 catctcccat ctatttgcaa acaataaaag gaattgggta tcaattcaac acggaggtat
    13141 gaaaatggat gaacaaacga taattctttt cttactcttg ctgtcgattg gtctactttt
    13201 tctttccata tttgcttttt ccaagcttgg gcatatttac aagcgcctaa aagatataga
    13261 ggagatactg gctgatgtac aaattggaga cgaaaatcgt aaaatactaa ttaagccatg
    13321 tgatgttatg gctccattgg tttatcagct taatgagatt gtctacgatt atgaaaataa
    13381 gttactttct ctgaaaaagt cggataaagc tagcaaacaa cttatgacaa gtttatctca
    13441 tgatgttcgt acaccattaa caactctgat tggttatctg gatgctgtac acagtggtat
    13501 agttatcgaa caagagcggg aggaatacct tgaaatcgct aggcgtagag cctacgacct
    13561 caaggattac attgatgttc tttttgactg gtttcggttg aactctgatg aatttacgct
    13621 gtcaatagaa tctgtggaaa ttgcagagtt gtcacgcaat attttgaaag actggattcc
    13681 tatattccat gaaaagaaac tgaattttga aattgatatt ccagaaaatc gactaatggt
    13741 aaacctcgac ccagatggat attcccgtgt ggtcaataat ctggttcaaa atgttcttgc
    13801 tcacagtaag gctcggcaaa tcaagattac catgagtgag gattccagaa tggtattact
    13861 ccgtgttgag gatgatggtg ttgggattgc cagagaaaat ttaccacata tttttgagcg
    13921 actgtataag tgtgataaag gacgttcaga aaaaggaagt ggtcttggtc tatctattgt
    13981 cagtcaaatg gtggagcgaa tgggtggtca ggtttccgta gaaagtgaga ttggaaagta
    14041 taccgtattt acagtgagct taccattaat ataaaattgt ttttccccca tgtgacatgt
    14101 atacacatgg ggattttttg taagttataa agttgcaagg ttaatacaag gttgttgcaa
    14161 ggttaatgta agattgaggt ggtatgataa ctttaataaa caaaggaggc ttttattatt
    14221 tatgaataat ttaattattg aaacaaaaaa tctcactaag caatatggtg ctcaaaagag
    14281 cgtttctaat ttgaatattc acgttcagaa aggtcgcatt tacggattgc taggaagaaa
    14341 cggtgcgggt aagacgacaa ctatgaaaat gttattgggt ctaacaaaac caactactgg
    14401 agaaattaaa atatttggtc aggacattca caataacgag aagaagcttc ttcctcgaat
    14461 tggatgtttg attgaatcac cagggtttta tcctaatctg acaggaacag aaaatctcaa
    14521 aatctttgca aggcttagag gaataccaaa aaaaaatgca gtagaatcag cactggatgt
    14581 agtcggtttg ccaaacaatg acaaaaagtt attttctcaa tactctcttg gtatgaaaca
    14641 gaggttggcg attgctcttg ccattatgca tgacccacaa ctgttgattt tggacgagcc
    14701 tatcaatgga cttgatccta ttggtattgc agaagtaaga acgtttattc gtgatctctg
    14761 cacagaacga ggtaaaacta ttttgatttc cagtcacatt ctttctgaaa ttgccttgtt
    14821 ggcagatgac attggcatta ttgaccatgg tattttgttg gaggaagaaa gtcttgccca
    14881 attagaagaa aaaaacagta aatacataca ttttgctgta tctgatgcag cacaggcagc
    14941 aaggcttctg gaaacccaac ttaacctgaa aaatttcaaa gtagcagata attacaatat
    15001 tatgctatat gatacagatg tttctgttgc agaaataaat cgtgctttca ttctaaacaa
    15061 cattgaggta tcagaatctc atttatgcga agatactctt gaagattact tcaagaaagt
    15121 aacagggggt gaaggaattg cttgatataa tttcatgtga attttcaaaa ctcaagcggt
    15181 taaaatttat actgatttct attttaggtg cttgcttatt tccaattccc gcaacaattt
    15241 tgattgcaaa ggacaacttg ccctttgagc aattgttcaa attagttgtc aattttggat
    15301 attttttgct attaccaatt gtattaagta ttgttgctag ccaactattt tttatagaaa
    15361 gagataatga tgtattaaaa aatcttgcaa cagtgccagt gcaaaaagga aaactggctc
    15421 tggcaaaatt ggctgtgttg ctgtttatat cattgttcta ctctgttgca gggttaggag
    15481 ctactatcat tggaggtttc attgttggaa tagtagaggg ggttgctgta aaattaggaa
    15541 tgagcattgc ttttggaatt atgttttttg ttactgtact tcctgttgtg gtactcattg
    15601 tatatttcaa tcgcagctat attttttcta ttatactttc ttttgtatat gctattttca
    15661 attttagtat atccttaaat attatcaatt ttgaaccaaa taacccaata ttaagtgtat
    15721 tacctgctcc tgttataatg cgttggtgga tggcattttg gggagatccc actggcgaat
    15781 acacggcact tagacagcca tatttgctct caactcctgc ttgtgtaggt attttatgtt
    15841 tgataaccat tatagcagtc ctgctgattt gtatgatata caaaaagcag gagaattagg
    15901 aggtatctat gcttgatata attactacag aatttcaaaa aataaaaaga tataatattc
    15961 tatggattgg tgttgttgct gttctgtttt cagcactatt ggcagttttt caggttgtaa
    16021 gttcacacgg cagtgaccct ctaacgtatg agagctttgc aaacggagtt atctggaata
    16081 atttcagtct tagttttcca tttgggatta cacttattgg aggatatctt attaaccgtg
    16141 aatatacaga ccaaacctta aaaaacatcc taactgttcc tatttcatta cgcaaattgc
    16201 tagtgggaaa attaattgcg gttggaggaa ttactgtatt gttaagtctt ttcagcttct
    16261 tatgcacatt tattttgggg ttgttgtttt gcaatatcaa tatatctgtt acactcgttg
    16321 taaaatcctt agttcagatt ttgtctgtta atctgtattg ttatgttgct gttttgccga
    16381 ttattgctta ttttggtcgt aaacaaaatg catttctgac gggtgtaggg attgcatttg
    16441 tatacggttt ttgtggtgtg tttgttgctg gaagaaatct gacagacttt tatccaatca
    16501 cagcaggtct tggtctagtt ggatacaaca atggtgctgg tactgtttat cagccactta
    16561 tcggtgctat gactttgatt gtaattctaa tcttgacaac tattctactt gtatttacac
    16621 ctaattatga taaagttatg accatatcta agaaaaaagg gataaagcat aaaaagtatt
    16681 ctcataaata acataaatat tgtttctatt atttaagttg ctagacagtg aaaagaaaaa
    16741 atctgtcgta cagtgtctat atgctcatgt attcaagaag ctacagtttt gatttttcat
    16801 aactgtagct tctttttgta tttattaaat cacgactatc ataatacttg caataggtat
    16861 agcgacaaga gagagtactg tcatatgtgt tttatatatt acattaagta tattctaaaa
    16921 tatatgtttt aaataatata tttgaaaata ggaatattta aataaaaaaa ataatattat
    16981 atatagttga caaaaattgg caaatgagat aatatataaa tatagaaata gtaatcatta
    17041 ctattattaa aaagggagga attaattatg aacttaaaag gaactaaaac agaaaagaat
    17101 ttaatggcag gatttgcagg agagtcagaa gcaagaaata aatatacata ctacgcttca
    17161 aaagcaagaa aagaaggtta taatcaaata gctgctatat ttgaagaaac tgctaacaat
    17221 gaaaaggaac atgctaaatt atggtttaaa cttttacatg atggtatgcc ttcaacagag
    17281 gaaaatttaa aggatgcagc agcaggtgaa aactatgaat ggactgatat gtatgcaaaa
    17341 tttgctaaag aagctagaga agaaggattt gacaagatag cttatttatt tgaagctgtt
    17401 ggaaaaatag agaaagagca tgaagaaaga tacttaaagc ttttagaaaa cttaaatgaa
    17461 ggaaaaatat ttaaaagaga tgaagaagtt gtttggcaat gtcaaaactg tggtcatgtg
    17521 tatgtaggta ctgaagcacc agaaaaatgc ccagtatgtg accatccaaa agcgtatttc
    17581 aatataaaag ctgaaaatta ttaaaaatta tcattattaa aaaggaatgt taactatgaa
    17641 attttctaaa caacgagaac tgattttaaa tgaaatatta aataatccgg ttcatcctac
    17701 tgcggattac ctatatgaaa acttaaaaaa agataatcca aatttaagct taggaactgt
    17761 gtatagaaat ttagctcagc taacggaaca tggctttata agaaaggtta gtattccagg
    17821 gtatccagat agatttgatg gtcgaataga taatcactat catatcatat gtgaagtatg
    17881 tggagaggta tacgatttag aatccgaagt tcttaataat ttacaagaat taatatctga
    17941 ggagacagac ataaaaataa catcttataa cataagtttt aaagggattt gtaataattg
    18001 taaaaggtgt agccaagtag gttagttttc aaattctaaa aataattaat gaggtgagtt
    18061 tatgtgtagt gaacaaaaat tttttatatg tgagacttgt ggaaaccttg taggaatgat
    18121 acaaagtggt ggtgtaccta ttttctgttg tggaaaacct atgaaggaac ttgttcctaa
    18181 tacaacagat gcagcagttg aaaaacatgt acctgtaatt gaagtggatg gaaataatgt
    18241 tactgttaaa gtaagtagca caactcatcc aatgactaaa gaacatcata tagcatgggt
    18301 gtatcttatg actgaacaag gtggtcaacg caaatgtcta gcagtagatg gagaacctgt
    18361 agttaaattt gctttgaatg atgatgataa agtgatttct gcctatgctt actgtaattt
    18421 acatggatta tggaaggctg aactgtaata tttaaataaa ttgagtcaaa aaggaggcta
    18481 taaatgtatt ttcatacaaa tagcctccta taaatttata taaattcaag ttttaatttt
    18541 gtttaatgtc aatataatat aaggagaaaa taatatgtca atttataaat gtagtgtttg
    18601 tggatatatt tatgatgaat ctaagaatga taaaacatgg gatgaattaa gtgaagattg
    18661 ggaatgccct gtatgtacaa aaggtcgtag ttattttgga aagataagta ctgtttacta
    18721 tgaagaagat gaaaagatag cagaagacat agttgaagat gaatctaaat taaatactga
    18781 aaaagaggga gatttaaatt acttaagtac atatttaaga agagatgatg aagttgagaa
    18841 acatatggat ataattcacg aaatggctgt tacaggaaaa tctataatag aacctatgag
    18901 aactaaacta ccagtaattt cttgggatga tattttaatt atgggtgctc aattaaatcc
    18961 attaccttta aatgagcatg atgaagtaaa tacaacaaca ataataggaa aaaaagcaaa
    19021 gaaacctatg attatagaaa atccagttta tatttcccac atgtcatttg gagctttgtc
    19081 taaagaacta aaaatagctt tagcaaaggg tgcagcccaa aataagacag ctatgtgtag
    19141 tggtgaagga ggtatattac cagaagagaa ggaagcttca tacaagtata tttttgagta
    19201 tgtaccaaat aaatatagtg taactgaaga aaatcttaaa aattctgatg ctatagagat
    19261 aaaaattggt caaggaacaa aacctggtat gggaggtcat cttcctggcg aaaaagtaac
    19321 tgaagaaata gcaaaagtaa gaaataagcc tgtaggtcaa gatgtaataa gtccatcttg
    19381 ttttgaggaa atacaatcca aagaagattt aaagaaactt gtagatgaac ttagagaagt
    19441 ttcagaaggg cgtccaatag gtgttaaaat ttcagcaggt catattgaaa aagatatgga
    19501 atttatagct tatgctaaac ctgattttgt aactattgat ggtcgtggtg gagctactgg
    19561 agcaagtcca aagttgctta aagatgcaac ttcaattcca acaatatttg ctctttatcg
    19621 tgctagaaaa tacatagata cacatggatt agatatagat ttggttataa caggtgggct
    19681 tcgtatatct actgattttg ctaaggcaat tgctatgggt gctgatgcag ttgctattgc
    19741 aagttctgct cttatggcag cggcgtgtca acaatatcgt atatgtggtt ctggtaagtg
    19801 tcctgtagga gtagctactc aggatgagga attgagaaaa agattacata tagaaaacag
    19861 tgctaataga gtggcaaact ttttaaatgt aagtttagaa gaacttaaga cttttgctag
    19921 aatttctggt cataaagata tacatgattt aagtgttgat gatttatata ctgttaactc
    19981 tgaaatatca aattatacaa acatacagca tgtatagatt gaagtttgaa actattagat
    20041 tttagatagc tataaaagta aatgataaat atattttata aaagagagtg tcttaaaatg
    20101 aactttttag ttcataagtc actctttttt ttatgaagcc aaatatattt atcattttag
    20161 caatgaaaaa gaatcttatc tctttatttt gagatagact ccttctattt atgaaaatta
    20221 aagatattaa attgtaaaat attctgtgat gtcgtactat taaactaaag attctgtact
    20281 ttaaagtaaa taaaaattta aggagtgatt attatggaaa gattgaagat acaagtttta
    20341 gttgacaata atacttatat tgatagatac tttgttggag aaccagcagt aagctattat
    20401 attgagattg atggaaatag gattttgttt gactcagggt attcagatgt attcatatca
    20461 aacgctgaaa aattaaatat agatttagga aatttaactc atgttgtttt ttctcatggg
    20521 cataatgacc atacaagagg tatccagttt ctagaaaata ggtatgattt atctacagtt
    20581 gaacttattt cacatcctaa ctgttttata cctagaaaac atggagagaa aagcatagga
    20641 gctccatttt ctgctgagga aattaaaaat atttttatgt ataatcctaa ggataagcca
    20701 tttaatctaa gtaagaattg tgtgttttta ggtgaaatac cttctataaa tgattttgaa
    20761 aaaagagcta aaattggtaa atgtaaaata ggagatttgt gggaagatga ttatgtttta
    20821 gatgattctg ctatagtttg taaaactgat aaaggtattt ttattgttac aggatgttct
    20881 catagtggaa tttgtaatat agtagagtat gctaaaaagg tatgtggaga tgatagagtt
    20941 attggtatac taggtggttt tcatcttttt gaattgaata ataggttaga tagtacaata
    21001 caatatttgg ataaagaaga aatgagaatg ttgtaccctt gtcactgtgt ttcttttaag
    21061 gtaaaagcga agatgaatga aatattgaat ataaatgaag ttggagtagg gctgaccata
    21121 agtatatagt atttgaaagt atataagatt taagttgaag gttaaaaaga tattttctat
    21181 agaaaatatc ttttttgtta cgtttatttt aagagaaatc aatatacttt tatatgaaat
    21241 attgtaaaaa aatttatttt taatgagaat atattttcat taataaacaa ttttattatg
    21301 tattaataat gttagaataa atataaataa atcaaagtta cttacattac agtatatttt
    21361 ttcacaaaat atattgattt attcttttaa agtttgataa ttccaatgaa tgta
//
LOCUS       DAYATN010000057        21412 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.057, whole genome shotgun sequence.
ACCESSION   DAYATN010000057 DAYATN010000000
VERSION     DAYATN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.057"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(250..510)
                     /locus_tag="ACGI8A_002018"
     CDS             complement(250..510)
                     /locus_tag="ACGI8A_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HFH0180015.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFRYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            727..2010
                     /gene="hflX"
                     /locus_tag="ACGI8A_002019"
     CDS             727..2010
                     /gene="hflX"
                     /locus_tag="ACGI8A_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFH0180016.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHFVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYANLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDADLDEEDFNIYREYILEK"
     gene            2018..2695
                     /locus_tag="ACGI8A_002020"
     CDS             2018..2695
                     /locus_tag="ACGI8A_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFH0180017.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            2781..3224
                     /locus_tag="ACGI8A_002021"
     CDS             2781..3224
                     /locus_tag="ACGI8A_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFH0180018.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            3529..4173
                     /locus_tag="ACGI8A_002022"
     CDS             3529..4173
                     /locus_tag="ACGI8A_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFH0180019.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(4259..4636)
                     /locus_tag="ACGI8A_002023"
     CDS             complement(4259..4636)
                     /locus_tag="ACGI8A_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFH0180020.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(4636..5022)
                     /locus_tag="ACGI8A_002024"
     CDS             complement(4636..5022)
                     /locus_tag="ACGI8A_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFH0180021.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            5201..5956
                     /gene="nadE"
                     /locus_tag="ACGI8A_002025"
     CDS             5201..5956
                     /gene="nadE"
                     /locus_tag="ACGI8A_002025"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFH0180022.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            6039..6758
                     /locus_tag="ACGI8A_002026"
     CDS             6039..6758
                     /locus_tag="ACGI8A_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0180023.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(6925..7929)
                     /locus_tag="ACGI8A_002027"
     CDS             complement(6925..7929)
                     /locus_tag="ACGI8A_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFH0180024.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            8195..9091
                     /locus_tag="ACGI8A_002028"
     CDS             8195..9091
                     /locus_tag="ACGI8A_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HFH0180025.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            9212..10474
                     /locus_tag="ACGI8A_002029"
     CDS             9212..10474
                     /locus_tag="ACGI8A_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFH0180026.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            10550..11743
                     /locus_tag="ACGI8A_002030"
     CDS             10550..11743
                     /locus_tag="ACGI8A_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HFH0180027.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            12372..13166
                     /locus_tag="ACGI8A_002031"
     CDS             12372..13166
                     /locus_tag="ACGI8A_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFH0180028.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            13261..14601
                     /locus_tag="ACGI8A_002032"
     CDS             13261..14601
                     /locus_tag="ACGI8A_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFH0180029.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14738..15970
                     /locus_tag="ACGI8A_002033"
     CDS             14738..15970
                     /locus_tag="ACGI8A_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HFH0180030.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            16010..17152
                     /gene="acrC"
                     /locus_tag="ACGI8A_002034"
     CDS             16010..17152
                     /gene="acrC"
                     /locus_tag="ACGI8A_002034"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0043958 - acryloyl-CoA reductase (NADH)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HFH0180031.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            17163..17957
                     /locus_tag="ACGI8A_002035"
     CDS             17163..17957
                     /locus_tag="ACGI8A_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HFH0180032.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            17971..18990
                     /locus_tag="ACGI8A_002036"
     CDS             17971..18990
                     /locus_tag="ACGI8A_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFH0180033.1"
                     /translation="MRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLAD
                     QLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERKK
                     PEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMATI
                     VCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSVN
                     LTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGTT
                     VRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEMI
                     ESLNR"
     gene            19089..20465
                     /locus_tag="ACGI8A_002037"
     CDS             19089..20465
                     /locus_tag="ACGI8A_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFH0180034.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20797..21141)
                     /locus_tag="ACGI8A_002038"
     CDS             complement(20797..21141)
                     /locus_tag="ACGI8A_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180035.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT         8042 a         2527 c         4111 g         6732 t
ORIGIN      
        1 tttcactctg gaatgatgat tttaatatta attttgttag taagtatagt actgtttgta
       61 atgtataaat tatgtaaatc tttatcaata aaaatatttc aatgttaaat agagggatat
      121 ttttatttcc aaaataagat aataaaaaag gattagatta aaagttatac aataaaaata
      181 gctgtgcaat aatatttaga ctatgaaagt agttgaaaaa tatttatgca cagctattta
      241 taatagaatt tactttttac tagtgatttt aactaaatct tgaaatttac tcataatgta
      301 attgtaatta tcttttttat gaggtaatat acaactactg caatctttga tacctttatc
      361 tgtataccta aagtttccac cacagttttc tcctaaagca tacaaaggac aatagcaaaa
      421 tagacagtta aactcttctg gtttattagt tttatgacaa gggaaaaatt cgcaatcttt
      481 gtgattaaaa aacttataat tttcgcccat attttcctcc taattaaata aaatttaaat
      541 tttttatact atataagatt ttggtgataa tataattata ctaaataatt ttatgaatag
      601 tcgataatat agtattatta atcatagtat taatggtata attagtcaac agaaaaataa
      661 tgtaaaaata atgtaagtgt attttgtaat tagaatatgt tttataacaa aaaatgaggt
      721 gattaagtga aagaaattca agaaaaagca cttcttgtag gtctgaattt gactacaata
      781 gtgaaaaaaa atgatgatat agacactaat gaatctatgg aagaattaaa agaactaaca
      841 aaagctgctg gggcagaagt tgtaggtagc ttaattcaaa ataaacactt tgtagatgca
      901 gcttattata taggaaaagg taaagtagaa gaaataagag cctatagtga ctctttagat
      961 gctactttag taatttttaa tgatgaatta tctggtgctc aaatcagaaa tatagagaat
     1021 gttgttggaa gaaaagtaat agatagaact actttaattt tagatatatt tgctcaaagg
     1081 gcacttagca aagaaggtaa attacaagtt gaattagcac aattaaaata tagattgcca
     1141 cgactttatg gaatgggtgg tgagatgagt agaacaggtg cagggattgg tactagaggt
     1201 cctggagaac aaaaactaga aatagataag agacatatac ttaataaagc agcagatata
     1261 agacgtgaac ttaaagaagt aaaaaagaat agagagacac agagagttaa gcgtttaaag
     1321 tcaaatatac cgattgtagc acttgttggg tatacaaatg caggaaaatc taccctgttg
     1381 aacgaattaa taaagacaca caaggattat gaacaagaaa aagaagtttt tgtaaaagat
     1441 atgctttttg caactttaga tgtaactttg agaaaggctc ttttgccgaa taaaaaagaa
     1501 tttttagtag tagatactgt tggatttgta agtaaacttc cccatgattt agtagaagcg
     1561 tttaaggcaa cattggaaga agtacagtat gcaaatttaa tcttacatgt aattgatgca
     1621 acaaatacta gttatgaatt gcaaaaaagt acgactgaag gtgtattaaa agaacttggt
     1681 gtaaatgata aaaaacatat acttgtatat aataaagtgg acaagctaga attggatata
     1741 taccctaaaa gtcaagaaga tattgtgtat atttctgcaa aacaaggcat aaatatggat
     1801 aagttattaa atatgataga aattgcttta atggaaaata cttattcagt aagtttgatg
     1861 ttaccttatg agagaggtga tatatttagt agaataaaag ataaatataa tgtggagaat
     1921 tttgagtatg gggaaagtgg tataacgcta gatgcagatt tagatgaaga agattttaat
     1981 atctatagag agtacatact agaaaaatag gttgaatatg gatataaact gggaaaatat
     2041 agataatctt gaagattatt ttataaccta tcttctttat aaagaatcta aaacagtatc
     2101 tcaaattagt aaaattagaa atatttcaag tactgaagca agtgagcaac ttatacaagc
     2161 aaagttaaaa ataaaggaaa tgcaaaaaga tgattttgag gcatctaagg atattttgga
     2221 taaatttcta gaactagata aaattaagag acttgatttt atggatagct tagatgacga
     2281 aaaaatggta tactttaaga gaaaggtttt caaaagaatc cttgtagaaa aaaatgctga
     2341 agatttaatt gttttaattt gggcgacagg tgagctaaaa gatgataggt ttttgaaatt
     2401 gcttcatcag ctcacaaacc acagacactc agatattaga agaataactt attcagcaat
     2461 cagaaagatt gaatcaccaa gtagtagaga agtactgcaa aaaggtctct acgacaaaaa
     2521 tgcacagact aggcagtact gtgcaaaagc tctttcaaaa ttgggagatg aaaacagttt
     2581 aaagatttta cagcagttga aagacaagaa taagaatttt gaaaaagaat atgtgcttag
     2641 agcttatgac gaagccatta agtcattgga aaatgcaaaa aatttgaaaa agtaattttt
     2701 tattaaagca atttgtttac ttaatgatgt atttctaaag tttaacaagg taataaataa
     2761 attaatggga ggggtttcaa atgatagtaa gacgttgtgc aggtggagta gtgttttatg
     2821 ctaataaagt acttattgta aaaaatgata ggggtgaatg gacacttcca aaaggaaaga
     2881 tactaggagg gggacttcca tatgagagtg ctgttcaaag agttaaagta gaaactggca
     2941 tagatgctaa aatgatagat gtggctggcg atactatgta tgaatttttc tcaagaagta
     3001 gacaacaaga agtttgtaat gcaataatgt ggtatgttat ggaagcttgt aacactgatt
     3061 gtgtacttgc tcctgaattt caagaaggtg gattttataa agtaaaggat gctttagaaa
     3121 tgttatcaca tcataaagag caagctttag tagaagtatc ttataaaaag tttaaggaat
     3181 taaagaaatt agtttcagag ggcgatgatg ttccagcaaa ttaattgtga atttagagga
     3241 ttctgaggaa gggatagccc cttccttttt taattgaagc agttttaagc gtaaattata
     3301 tagtctcttt aaactatgat ttatcaattt agagatttag tagtttatat ttaaaatcag
     3361 taagatatat ttaaaataaa aattaaaaag tatacaattt tattatataa ttgtcttatg
     3421 atgttgatta aagaagtatt aaaagtccat ttatttttaa atagaatgat tttataaata
     3481 tatatttgat gtgtatatta gaaaatgatt aaaataggag gaaatacaat gagcaattac
     3541 aggacattac atgaatttgg aacggatgaa ataacgattg aaaagtctgt ttttattgga
     3601 tatgctaagc ctataaagag tgaagaagaa gcattagagt ttataaatga aataaagaaa
     3661 aagcataaag atgcaactca taatgtgtgg gcttatactg taggtaaaaa tatgaatata
     3721 caaagatata gtgatgatgg agaaccacaa ggaacagctg ggatacctac attagaggtt
     3781 ataaaaaaag aagatttgag agatgtagcg gttgttgtta ctaggtattt tggaggaaca
     3841 aaattaggtg caggtggtct agttagagct tatacaaaag gtgctaaact gggacttgaa
     3901 gcaggtaaga taatttataa ggtaatgtat caagaagtta aggttaagat agattatact
     3961 caacttggaa aagtgcaaaa tgagttaatg aatttggggt attttataaa agatactgta
     4021 tatgaggata atgttgaaat agtagtatat tcaagattag aagatgtaga gaaattatca
     4081 gaaaaaatga ttgatataac aagtggaaca ggaaaaatag ttttaggaga ggaattttat
     4141 ttgtctgaac aaaatggaga aattttaata taatctaaaa ggttattaga atagttaata
     4201 cctcaaatag ccaattagta tttttaagtg aaaagccatt aaattgattt aatctaaatc
     4261 aatttaatgg ctttttatcg gattttgcac agaatccttt ttttacttca aataaaccga
     4321 ctaaaagtaa aaatatccca aatatttttt ttaatatatc tgaagacaga taattagcta
     4381 atagtgaacc aaatacagct cctaaaaccc caaacacagc tataggtata accaatttaa
     4441 aaattacatt tttatttttt atatgtatag caagtgctat aagtgtcata gggatagatg
     4501 aaagtaaatt tacgctttgt gctattttag tgtctatgcc agcaagtaaa acgagtgcag
     4561 gtataaggat tgttcctcca cccattccca ttcctccaat aattcctgca aaaaatccaa
     4621 ttagagcaaa taacattaaa acaccatcct taatgctgca attatcataa ttattccaaa
     4681 agaaattcta agaaactttc cagtaacctt atttagtaat ttagctccta taataccacc
     4741 aagaatactt ccaactgcaa ctttaattgt taagttaaca tcataagtac ctttagatac
     4801 atataaaaca gaacttgctg tcgttaagaa tattattata gaaagagcag ttgcatgaga
     4861 tttatgttct tctaccttaa cgatgtcatt taatattgga actaaaagag tacctcctcc
     4921 agaaccaaaa actccattta taaatcctgt aaaaactcca attatcgtat tttttaaatt
     4981 aaaatcaaag tattttttct ttaaatcatc agtttttttc ataaaaatca ccttctgaaa
     5041 tagttttacc aaaataggca aattatattt atataaaata agattaaata taatctttca
     5101 tgtttgttta attttgcata aaaaaaaaca tattaaaaca acatattaat atatggtata
     5161 atatattaaa acaaagtagc aaatttgaga ggagataaca atgtcaaata taaaaataca
     5221 aatagacaaa actgtagaat ggcttataaa taaggttaat gaggcaaatg caaaaggatt
     5281 aattgttggt gtatctggag gaattgattc ggcagtagtt gctaatttaa ttaaaaaggc
     5341 atttccagaa aactctatgg gagttataat gagcataaaa agtaatcctc aagatagaga
     5401 agatgcactt aaggttatag agggatgtga tatagaatac cttgatttag accttataga
     5461 accacaaagt gctatattag acatggtagt aggaaattta aaagataaac atttatatag
     5521 agaagaatat ttaaagatga cagatgcaaa cttaagagca agagtaagaa tgagtacaat
     5581 ttatacaatt gctaacaatc taggatatct ggttgttggt acagacaatg ctgcagaaat
     5641 acacacagga tattttacta aatttggtga tggtggagtt gatattttac ctattgcaaa
     5701 tttaactaaa ggagaggtgt atgaatgggc aaaagagctt ggtgttcatg aagatttaat
     5761 aaacaaagct ccttcagcag gtctttggga aggtcaaaca gatgaagatg aaatgggaac
     5821 tacttacaat atgatagatg ctgtattaga aggtagattg gatgaagttc ctaaaagaga
     5881 ccaagagata attgaaagac ttcatagatt aagtgaacac aagagaaaaa cgcctgcaca
     5941 acctcctaag ttctaattta ctatttataa gatttgtgat aaaatttaca agtgatgaaa
     6001 attagaataa ttaattgtac gtattaagga gtggaaatat gggacgtata ggtaatataa
     6061 ttaatagaaa aggtaaacaa gatgctcaaa gagcaaagat atttactaaa catgctagag
     6121 caatagcagt tgcagctaag gaaggtggag cagacccaga gtacaatgca gcgttaaaaa
     6181 cagctataga aaaagctaag gcagataata tgccaaatga taatatagat agagctatcg
     6241 ctaaaggtgc tggagcaggt gctggagaag actatgaaac tatagtttat gaaggatatg
     6301 gacctggtgg agttgctgtt atagtagaaa ctttaactga caacaaaaac agaactgctg
     6361 gtaacgtaag atactatttt gacaaaaacg gaggaaactt aggaactagt ggatgtgtgt
     6421 ctttcatgtt tgataaaaaa ggacaaatat tagttggttt aggagatgga gtttcagaag
     6481 aagaattgat ggatgttgca ttagaagcag gagctgaaga tttcataaca gaagaagatg
     6541 gttatgaaat aataactaca ccagaagatt tttctagtgt tcgtgatgag ttaaaagcta
     6601 aaggatatga gtttatatct gctgatgtga agatgatacc tcaaactaca actgtattga
     6661 ctgaagaatc tcatttaaaa atgatgaata aattagttga tatgcttgaa gaagatgatg
     6721 atgttcaaga tatatatcat aactgggaag tagaataaat agtataagca tattgaaatt
     6781 tataagtatt gaatcaattt taacatgtga taaattttat agaataagaa acactaaaat
     6841 agacatacgc acctctaacc tatatttgca taataatatg taagcatagg taaggggtgt
     6901 ttttatattt ataggttaag aaatttaaag taaattttga gctggactcg attgtctata
     6961 aataattgat gggtatataa cttgttgctg aataccattt atacgcccag ttactgtatc
     7021 aataatcatc ctaagactac gttctgctgt ctcttccatt tttaaatcta taactgtcaa
     7081 aggaggaata cttcttgcca ttagtcgtga aggtccatta cttgctgcaa atatttgaat
     7141 atcctctcca attacaattc ccgattcatg aaatgcattc atcataccaa gagctaccac
     7201 atcatgatta caaaaaatta ctttttttag tgaatttttt gcaagcattt tttgagcaat
     7261 atcatatcca ctatctattt tattagaaca ataatatata gaatctgata gatgtattcc
     7321 atgttcttta catgttttta aaaaagattt accccttaaa gtcatacatg taaaggaagt
     7381 tgggttgaat acaacagcaa tatcattttt accattttga atagcatgta aagctgctat
     7441 acgaccagtt tcctcattat caatagatac acttgaaaaa ccaggtattt cacgatttac
     7501 aacaactaca ggaattttag gtttattttt tactagatat tctatatcat cagcattgat
     7561 acctgttaaa acaattccat cataataatt atttgcaaaa aagtattcat catcacatag
     7621 atgaccaact ttgtaaggtc gtatgatgat atttatgttt aaatgttcta aagaaattat
     7681 gtggtttatt gcttgaagta tattggttga tgccaactca aatccctcat cctgccaata
     7741 aaaagctatt ttaatattat tatgtttaat aggagtagag cgtttatatc caagcttatc
     7801 agctgaattt aatataagct cttgtgtatg tagcggaatc ctgcgttcat ttcctttacc
     7861 acttaagacg tacgagacag ttgctgaaga aagaccacag tcttttgcaa tatctttgat
     7921 tgttgccatg ataaaatcct ccttaaatag ttgcttaaat ttgcttatac caataatctt
     7981 aacatatatt caactaaaga acaatattct acaaggtgca tattttacag gattagaata
     8041 aataaattaa aaaaaacttc ttacttatgt tgacataatt tagtttatag gattataata
     8101 tgcttaaata agcttatgat taacttgaat taagcaaagt taagcaggaa catgcaatgc
     8161 tagcttgcaa tgtgactgca aagggggaaa aggtatgagt aaggtaaata tatttgaagt
     8221 tggtcctaga gatggatttc aaaacctgaa agagttttta gaaactgata aaaagcttaa
     8281 gattattgat ggtcttattg cttctgggat taaacaactt gaaattagtt catttgtaag
     8341 cccaaaagca atacctcaga tgaaagatgc aaaagaagta gcaacttatt gtgtagaaaa
     8401 atatcctgat gtgaaacttt atgcattagt tccaaattta tatggtgcta aagttgcatg
     8461 ggagtgtggt ataagagata tatcctatgt tatatctttg agtgaaactc ataataaaaa
     8521 aaatataaat cgtacccatg aagagtctat tgatgagctt aaaaaaataa tgaatactta
     8581 tccagacatg aatatatgta ttggaatggc tactgtattt ggatgtcctt ttgaaggttt
     8641 accaaaaatt gaagatgcca taagatttgc taaacgcctt tgggaaaacg gtatcaggga
     8701 gataaatcta gcagatacaa tagggattgc tgaccctaag caagtaagag aaactattag
     8761 tgcattagtt gaagctttac cagaatgcag atggcaagta catattcatg atactagaaa
     8821 tatgggaatg gttaatactc ttgtggctat tgaatctgga attactactg ttcagtctac
     8881 acttggagga ttaggaggat gcccatttgc tcctggagca tctggcaata ctgcaactga
     8941 agatttagta tatatgcttg aacgtatggg gtatgataca ggagttgatt ttgttaaact
     9001 tcttgaatta tcaaaatatc agaagagttt tgtaaatgga gtatatagtg gtcatcatgt
     9061 aaatataaag tctgatagtt gtaattgtta gttctattga ttaatcgatt aaaaaaatat
     9121 gctgattggt aaaatagatt tattgataaa aaatataaat tataaaatac attatctaat
     9181 aaaacaaata aaaaaattga gggggattag tatggatatt atagctttac ttttattttt
     9241 aggagttatt attattgcat ttgtacgtaa gaataatgca ggtattttgg ctctagctat
     9301 tggtgtcata gctgttagaa tttttggaat ggatgataaa gaactttatg gagctataag
     9361 caactcaatg tttactacac ttgttggtat aacactactc tttgcaatag tgaactcaac
     9421 aggagcatta aagcttgttg cagaaaaaat tgtagctgct tctggtaaac gtgtttggct
     9481 tatacctata gcagttttta tagcaggatt tgttgttgct ggtgttggac ctggggcaat
     9541 tccagcactt gcaattatcc cagcaatggc tgttcctgta gccataacag ttggttataa
     9601 tcctgtaatg cttgcactta taggtgaatg tggattaatg gcaggtcgta tgactcctat
     9661 tactccagaa ggtcaaatta taacaaccgt tgcagaaggg gttggaattg aaaatgttat
     9721 gcctactatc ttagcctgtc aaactctttc tacaattgta ttttcaatag tattgtttat
     9781 aattttcaaa ggatataaac ttaaaaaacc tataaatgtt atagatagta agcaaataga
     9841 aaaattcaat ataaagcaga taatttctct aataagcata gtagttatga ttgcttttat
     9901 cataattttt gatatgaatg ttgggcttgc agcttttgca gtttcagctg tacttatttt
     9961 tttcaatatt gctgatgaag gagaatgtat caaatcaatt ccatggagca cgatagtaat
    10021 ggttcttgga gtaggtgcaa tgatgacaat cgtagatgca gcaggaggta ttgacttgat
    10081 gagtaatgca ttatcatcac tgatgaatag taaaacagct actccaatta tgagtatttc
    10141 agcaggactt atgtctatgg ttagttctgc tttagcagta gtttatccaa ctatgatgcc
    10201 tatgtgtgtt gacatagcta aggcagttgg aggagtagac ccactagcac ttattgctgc
    10261 agttggtgtt ggaggttctc ttgctggtgt aagcccactt tctactggtg gggcattgat
    10321 tttagcagct atgggttcaa gtaagaaaga ctttaataag gaagaacaaa ataaggtttt
    10381 tgtacagctt ttcattatga gtgcagttgg cttattggtt attgcagtag tatcaatatt
    10441 atcatttaat gcaataacac atttaatgaa ctaattagaa taactgaaaa taaatcatta
    10501 aatttaactt ttaatattaa tcaatataat ataatttgga ggggaagtta tgaaaaaagg
    10561 agcattaagc aatataaagg tacttgacct tacaagagtt cttgcaggtc catattgtac
    10621 aatgatgttg gcggattttg gagctgatgt tataaaaata gagatgccaa gaaaaggtga
    10681 tgatacaaga ggttttggac cttttgtaaa tggagagagt ttatactatg caaatataaa
    10741 tagaaataaa aaaggtatca ctctaaacct taaatcagaa gaaggaaaaa atatattttt
    10801 agaaatggta aaacatgctg atgttgtagt agaaaattac agaccaggtg taatggaaaa
    10861 gttaggtctg ggttatgatg agttggctaa agttaacaat caaataattt atggagctgt
    10921 atcaggattt ggatgttatg gaccatattc agaacgtcca ggttatgata ttattgcaca
    10981 ggcaatgggt ggtcttatga gtataacagg gcaagaagga ggagaaccaa cgagagttgg
    11041 taatgctatg ggtgatgtac taggtggaat gaatttaact attggtatac tcacagcact
    11101 caacgctaga aaaataatag gaaaaggaca aagagtagat gtttccctcg ttgattctgt
    11161 tgtatcgtct ctcgaaactg gtatacagag atactttgat tcaggacaac ctgaacttat
    11221 gggtaataga tatgcttcag cctatcctta tgactctttt aaggcaagtg atggaagttt
    11281 tgtaataggc tgtggtaatg acaaactttt tgaaaagtta tgtaaaaaag ttctttttag
    11341 agaagacctt ttaatagatg agagatttaa aacaaatctt aatcgttgtg aacattacaa
    11401 ggctcttaaa ccagaaatag aaaaatggtc tgttaagcat acaataaaag aagcagttga
    11461 cttaatatca gaggctggag ttcctgctgc tcctatctat aatcttgatt gtgttacaac
    11521 agatgaacat atagcaaaag caagagaaat gattgtagat attagtcatc ctattatagg
    11581 aaatatgaaa gtcaatggaa atccagttaa gttgatgaca acaaaagcta aaattgatat
    11641 gccagcacca tctctagggg aaagtaacga ggagatttat aatggttggc ttggttttga
    11701 tagtgataaa ataaaagatt taaaagaaaa tggagttatt tgattaaaaa atagctaaac
    11761 acacttgtga ctttagtcat gggaggttca gttagaataa tatcaaaaaa tattaaatca
    11821 atactgtaaa taaaatatag aattgtcaga agcatttaag atgggaaaag ctacttagac
    11881 attgagtctt tgtggctttt cctattttca ttaagaattt tatattatca gaatagatat
    11941 gtactaaact aaagtattaa caatttaaat taatagaaaa taaactacct aattatgttg
    12001 aataaagact actttagtga aaataaactt atagagatgc tatttaaatt attttaatgg
    12061 aaatatcaaa aatgagactg cattctcaaa aatgagactg cgttttttaa gtattttctc
    12121 gaaaatgaga caaaaatatt gttttatggg ttttattgaa gaaaaattct tctgtgaatt
    12181 acaatttaat caattttaaa gttgaaaatt attaaaaatt tagtgaaatt tatgtatatt
    12241 tcgtttattt tattaaaaat aacaattttt tactaaaaat aaagaacttc aatggtgatt
    12301 ggcatagtac ttgctatttt atattaacaa gaaatgtaga aaaaataaca atgtataatg
    12361 gggggaatat gatggaatct gtaatggaaa atttgaacaa tcttaaggtg gaggttaagg
    12421 acaaagtctg tgttattact ataaatagac ctaaagcttt aaatgctctt aatagtgaca
    12481 ctcttaggga actatcacaa gttattgatg tagtatcaga aaatgaggca atattaggag
    12541 ttattattac tggagaaggt aaagtttttg tagccggtgc tgatatacgc cagatgcaaa
    12601 actataaaag tgaggaaggc agaaaatatg caggttatgc tcagggaatt tttgataaaa
    12661 ttgaagctct tgagaaaaca gtaatagcag cagttaatgg atatgcatta ggtggtggat
    12721 gtgaacttgc tatgagttgt gatattagaa ttgcatcaga aaaggctata tttggacaac
    12781 ctgaagttaa tttaggagtt attccatgtt ttggtggaac tcaaagatta tcaagacttg
    12841 ttggaactgg aatagctaaa gagttaattt ttacggggag acaagtaaat gcagaagaag
    12901 ctaaatctat tggacttatt aataaagtgg ttccaagtga tttactttta gaagagtcta
    12961 tgaagatgat gaaccaaata gttgaaaaag cgccaatagc aataagatat gcaaaagttg
    13021 ttattaacaa agggatagat atggatttaa agaatgctct tgaactagag aaagatgttg
    13081 caggtttaac ttttgctaca agagataagc aagaaggaat gaatgcattt atagaaaaga
    13141 gaaagccagt ttttgaaaat aaatagctat tataactaaa attggaacaa tgtagaatat
    13201 attcaaaatc taaatttcgt gaaaaaatac atatagttta attttaaaag gagttaattt
    13261 atgaatacgg gagtaattat aagtttagta gggattatca tagctctatc tcttctagtt
    13321 gtaatggtta tgaagggaat aaatattttt attatagcaa ttgtatgttc atcattagtt
    13381 gctataacag gaagcctaaa tgtgtatgat gcacttaaag tggattatat gacagggttt
    13441 gttggatttc ttcaggcaaa tttctttatt ttcctaacag ggacattaat gggaaaaata
    13501 atggagatta ctggtggagc aaaatctatt gctaagatga ttgtaagatg gattgggaag
    13561 gataaagcac tactttctat tccaatagct tgtggaatat tagcctatgg tggagtaagt
    13621 gttttcgttg taagttttgc agtgtttccg atagctttag aaatttttaa agaagctgat
    13681 ttgccacgaa gatttatacc agcagctttg acttttggat gctcaacttt tgctatggtt
    13741 gcaccagggg caccacaaat tcagaatatt gttccagcat caacattagg gacagatatt
    13801 atggctggag cagtaaatgg ttttatatca tgtgcagtaa tgtttatagt tggaagtaca
    13861 attttgtaca aaatggtttc aaaagaaaaa gccaatggtg gtcattttat agcacatgaa
    13921 tcagatatat ttgatgaagc agaagcaact aattctaaga gagaaaatgg tcccaatggt
    13981 gtaattgcct taattccatt gatagtgtcg attttaataa ttaatgtaaa agttaatgga
    14041 aaagcgatag taccaattga ggtgggagta tttattggag ctttattagt atatttattg
    14101 ttaaataaat atcaagataa tactaaaata gttggacatg ttggtgatgc atgtaaaact
    14161 acagtagtag caatatgtaa tacttgtgct gtagttgcat ttggttcagt agtaaaatca
    14221 gcagttggtt ttgacttcat agttaatgct atgactaata ttcctggtcc tcctattgca
    14281 ggggctgcag ttggaactac agttattgca ggtatttgtg ggtcagcttc aggtggacta
    14341 ggaattgcag ctccacttct tggaccagta tttttagcca agggtgtttc cgctgcagca
    14401 ttaactagga ctatggcaat ttcatcagca gcattagatt cacttccaca caatggatat
    14461 attgttactg taacaaatgg tctttgtaaa gaaacacata aggatgccta tatgccaata
    14521 ttctgggtaa ctgttttaac cccatttatt ggaaccatag tagcagtcct actgtttaca
    14581 atgttcccgg gattgccata ggagaaaaga taaaaggtat aaattatatt ttgatattat
    14641 aaaatcaatt ttcaaaatat atacaataaa tttcatgttt taatactgta aatatgtatt
    14701 taaaataaca aattgaccat ataaggagga attgaaagtg ttaaataaac cattagaggg
    14761 agttaaggtt attgacctaa cgtattttgt agcaggtcca gggacttcta aaatattagc
    14821 tgactggggt gctgacgtta ttaaggtaga gccaagcttt ggagacccag gaagaaagac
    14881 aggggcaaca atgacaatgc caatagatga ttataataat cctttttaca gcacatataa
    14941 ttcaaataaa agaggtttgt ctataaatct taagagtgaa actggaattg aaattatgga
    15001 taagctttta agcgaagcaa acgtatttgt ttcaagttat agaacaggag cattaaaaag
    15061 actaggtctt gattatgaat cattaagtaa aaagcatcca caccttatct ggggtcagat
    15121 aaatggtttt ggcgattttg gacctgctaa ggataatgca ggatttgata cagttgcctt
    15181 ttgggcaaga agtggggcta tgcttgatat agcagaaaaa gatacaagtc caataaatcc
    15241 acctatagcc tttggagatg caacaacttc ttgtagttta tctggaggaa tatgtgcagc
    15301 tttgtaccag caagctaaga ctggaaaagg acaaaaagta atggcatctt tatttgggca
    15361 agcaatatgg aatgcatcat ctttattagc atctacacag tttagtgatg agtatccaaa
    15421 aactagaaaa aatgctattt caccagtaat aaattcttat cgttgtaagg atggaaagtg
    15481 gatattctta agtattttag agcatgaaag atattttgag gcactttgta gattatttgg
    15541 aagagaagac ttaataaaag aagagaaatt tgcatcttca gtagaaagta agaaaaatgc
    15601 tacagaactt attaatattt tagatggtga atttgcaaag tttactcaaa atgagatggt
    15661 tgaaaaactt actgctgctg atatagctca tgaaaaaatt caacatgtta atgatgtagt
    15721 gactgatgaa caggctattg ctaataacta tatatatgaa catacaagta aagatggaaa
    15781 taagacaatg cttgcatcta ctccagttaa atttggaaat attgaagtta acatgacttg
    15841 tgatgctcca cttataggag aacattcaga tgagatattg agagaacttg gatacagtga
    15901 ttctgatata gagactctaa ttgaatctgg aattgtaaca atgaataaag aagcatgtga
    15961 aatatgttaa taaacatgtg aagtatgtta ataaatagga gggaatttta tggattttag
    16021 attaacagaa gcacaattaa tgcttcaaag agtagcaaaa gaatttgcag agaatgaaat
    16081 agcaccaata gctgctgaaa cagataaaac aggtattttt ccaagggagc tttttagcaa
    16141 aatggctaaa ataggattta atggaattgg aacacctgta gagtatagtg gttctggtgg
    16201 ggcagatata gaaaaggtaa ttgttgttac tgagattgcc aaaaaatgtg cagcatctgc
    16261 agcaatatta tcaatacaca caatatatgc tcaagctatt ttgaaatttg gtactgagga
    16321 acaaaagaaa aaataccttc caatgatggc tgaaggtggg tgtgtaggtg cttttgcctt
    16381 aaccgaacct aatgctggtt cagatgctgc gagagctgca acaactgcaa ttattgatga
    16441 agaaactgat gaatatgttt taaatggaac taagtgtttt atttctggtg gaggtcaagc
    16501 agaatcatta ataatatttg cattgacaga cccatcaaag ggaataaagg gaatgtctgc
    16561 tatcattgtc gacaaaggta ctccaggttt ttcaatagga aaaattgaag aaaaaatggg
    16621 tattcatggt tcagaaactg cagagttaat atttgataac tgtagagttc caaagtctaa
    16681 cttattagga aaagaaggta aaggatttaa tatagctatg acttgtttag atggagcaag
    16741 aattggagtt ggagctcaag ctgtaggaat tgctgaaggg gctttagaag aaagtatcaa
    16801 atattctaaa gaaagagtcc agtttggcaa gccaatttca gctttgcaag gtattcaatg
    16861 gtatatagca gatatggcga caatggtgga atcagcaaaa ttattagtat attatgctgc
    16921 agatttgaaa gctagaggag aaaaacacac taaagaagct gctatggcaa aatacaatgc
    16981 gtcacgtact gcaagagagg ttacaaattt agcccttcaa atccatggtg gatatggata
    17041 tatgaaggat tatccattag agagaatgta cagagatgcc aaaataacag aaatttacga
    17101 aggtacttca gaaatacaca aagttgttat ttcaagagca gtactaggat aggaggaaaa
    17161 gaatgaatat agttgtttgc ttaaagcaag ttccagatac taatgaagtt aaaataaata
    17221 aagagacagg tactctaata agagatggtg taccaagtat aatcaatcca gatgatagaa
    17281 atgctttaga agaagctcta aaaatgaaag atgaactagg agcagtgatt aaggttataa
    17341 gcatgggacc tcctcaagct aaaagtgctt taaaagaagc tttggcaatg ggagctgatg
    17401 aagcttattt aatctcagat agagcttttg gtggttcaga tacttgggct acatctacaa
    17461 ttattgctgc tgctatagag aaggttggaa agtatgatgt aatattctgc ggaagacaag
    17521 ctatagatgg agatacagct caggttggac cagaggttgc agaattttta gggataccac
    17581 aagttactta tgcgaaagaa gtaaaggttc aagatgacaa attactagta actcgttata
    17641 cagagacagg ggattattta atagaagcaa agatgccagt tttattaact gctataaaag
    17701 agttaaacaa tccaagatat ccaagtgtca agggaatctt agaagcttat aataatggag
    17761 atgcaaagat tactgtatta acacttgcag atttagatgt tgatacaact caaattggat
    17821 taaagggttc acctacaaac gtttataagt cttttgtacc agttaaagac aagcataatg
    17881 aaataataga aggtataaat aaaaaagaaa aagctgagaa actaatagaa atactattcg
    17941 atttgaaact agtataggag tgttaagatg atgagagcaa aagttaatca aggtataaat
    18001 ttaaatgatt ataatggtgt ttgggtaata ggagagcaaa gggaaggtaa aattaatcct
    18061 gttacgatag aacttattgg tgaaggaaga aagcttgctg accaattagg aaaagagctt
    18121 gcagttgtga tagctggata tgaagtagag aaagaagtga aggaattact tcactatagt
    18181 gttgataaag tatattacat caatgaccct cttcttaaag atttcactac tgatggatat
    18241 gcaatttcaa ttgcaaattt aatcgaaaga aaaaagccag aggttgtatt ggttggtgct
    18301 acttctatag gaagagatat agcgcctaga attgcaggta aggttggaac aggtctaact
    18361 gctgactgta caaaacttga aatagattca acagataata agttattaca aacaagacca
    18421 gcatttggtg gaaatttaat ggccactatt gtttgtccaa aaaatagacc acagatgtca
    18481 acagttcgtc caggagttat ggcaaaagct gtaagaaacg aatcagagac tggaatttta
    18541 gaagttgtta ctccagaact tactgaaaaa atgattagaa ctagattagt agagatactt
    18601 ccacaagaaa agaaatctgt aaatctaact gatgctagaa taatagtatc agggggaaga
    18661 ggattaaaaa gagcagaagg atttgagctt ataaaagaat tggctgataa attgggggca
    18721 gagattggtg cttcaagagc agcagtagat tcagggtgga tagaacattc tcatcaagtg
    18781 ggacaaacag gaactactgt tagaccagaa ctatacattg catgtggaat atctggagca
    18841 atacaacatt tagcaggtat gagtgattct aaatatatag ttgctataaa taaagatgca
    18901 aaagccccta tttttagtat atgtgactat ggaatagtag gagatttata tgaaattata
    18961 cctgaaatga tagaatcatt aaataggtaa cttttagaaa acattgaata tgtagaatga
    19021 taagtcttat aatattgtat tgtaagactt attatttttg ctgaaggtta tagtggggag
    19081 atgaaacaat gaaagagcag tggtataagg atatatttgc aagagtatta tctatgactg
    19141 atgatggttt tattgttgta aatacaagtg gagtaataat agatattaat gataaatatt
    19201 gtgacttttt aggaaaagaa agaaaagata taataggaca aaatatacaa agtattatac
    19261 caaatacaaa aatgctagat gttatgaaaa ataagtattg tgaagaagga gctatacatc
    19321 attattcagg tggaaataca aaagaaaaaa gtgtaatagt aagtaggtct tatgtggaaa
    19381 atgacaatgg agaagtagtt gcaggagtag ctcaagttaa atttagattg caatcatttg
    19441 atgtagctaa aaagttaatg tcagaatata tggagctcca atattataaa gagcagttta
    19501 aggataactg tggttttgac aagctcatag gtgaaaatag agacttcata gaacttaaga
    19561 aaacaggtgt gaaagcttct aaaacaaact ttccagtact tttaacgggt gaaacaggca
    19621 caggtaaaga agtttttgct agagcaattc acaataatag cagtaggtca gataaaccta
    19681 tggttagtat aaattgcgct gcaataccag aagaattatt agagtcagag ctatttggct
    19741 atgacgaagg agcttttaca ggagcaaaaa agggtggtaa aaaaggtaaa tttttagttg
    19801 cgaataatgg tacaatcttt ttagacgaga taggtgacat gcctttgacc atgcaagcaa
    19861 aacttttaag agttcttcag gaaagtgaga tagaaccagt gggaggtctt aaaactataa
    19921 agatagatgt aagagtaatc tctgccacaa gaaagaattt aagtaaaatg gttgaagagg
    19981 gattatttag agaagaccta tactatagat taaatgtaat aaacattcat atgatggaat
    20041 tgaaggacag gcaagatgat attttattgc tagcaaatta tatattaaac aaattaaatg
    20101 ttgaatacaa ggaattgaag gttttaagtg ataaggttaa aaattgtttt attaactata
    20161 cttggccggg aaatataaga gaactacaaa atgtaataaa aagtgcctat gctgtaagtg
    20221 atgatatggt cattatgatg tgtgaccttc catctaagat ggataatatc agcagagtag
    20281 ctcaatgtaa tgtggatagc aattgttcaa ttcatgaaat ggttgagaat tatgaaaaga
    20341 gcttaattat tgatgtgtta aggaagtata attggaaatg ttctaaggca gcagaggtta
    20401 tgggaataca caagagtctt ttatataaga aaataaaaaa gtatgaaatt gaattaaata
    20461 attaagataa cttgaattaa ggcaatgcta aaaaagcgcc cttattcaaa gcgaactgta
    20521 taaactgttt tatgcagtta aaacgtgtaa aagtagatgt gaagtactaa tgtcaatcat
    20581 tagtactttt ttattatatt tataattact atctatattt cagtatcttt tgatatctaa
    20641 gattattatg tttaatttag tatataacaa aaccctctta gttatggaca gttttttata
    20701 ttttaactgt ctactaagag gggatagttt agtttaaatt tagagtaaat tttatctagc
    20761 atttataaaa aatattttga atagcttttt tgttatttag tcataatttt ctacttcttt
    20821 atcaagttct ttgccacaag aatcacactt atatatagca ttgtatccgt aatcatattc
    20881 atcaactatt ttcttttgtc taaatacacc accacactca cataggaaac caatttttat
    20941 aagtgtaacg ataaattgtg attttataaa ttttatatca ttgcatttat cgttagtgta
    21001 aaatgtataa atataagagt tatcgcactc atctcttaat tcaaatcttt caatatatgt
    21061 gttatttttt aattcctcaa attcttcttt agttaaatat gatttttgtt gcaattcatg
    21121 taaacgctta atttggtcca ttatttatat cctcccaata attttagtat tttaagctta
    21181 gataaggaat ctaagtttta ttaaatttat cccaaggaat tatttaaatc atatttagtt
    21241 ttattaatta caacatatat aaattacacg aaaataaaac taattgtaaa aataatatcc
    21301 tgtgaaaacc taaactacta accccttact aacccgaaaa ctaaaaaaag aaaatatatc
    21361 caaatataag tgatatataa ataaagatgt ttcatataaa tttaacttta aa
//
LOCUS       DAYATN010000058        20764 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.058, whole genome shotgun sequence.
ACCESSION   DAYATN010000058 DAYATN010000000
VERSION     DAYATN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.058"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="ACGI8A_002039"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="ACGI8A_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFH0180036.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="ACGI8A_002040"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="ACGI8A_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFH0180037.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="ACGI8A_002041"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="ACGI8A_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFH0180038.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="ACGI8A_002042"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="ACGI8A_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFH0180039.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="ACGI8A_002043"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="ACGI8A_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFH0180040.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="ACGI8A_002044"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="ACGI8A_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFH0180041.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="ACGI8A_002045"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="ACGI8A_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFH0180042.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="ACGI8A_002046"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="ACGI8A_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFH0180043.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="ACGI8A_002047"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="ACGI8A_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFH0180044.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="ACGI8A_002048"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="ACGI8A_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFH0180045.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="ACGI8A_002049"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="ACGI8A_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFH0180046.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="ACGI8A_002050"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="ACGI8A_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFH0180047.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="ACGI8A_002051"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="ACGI8A_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFH0180048.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="ACGI8A_002052"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="ACGI8A_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFH0180049.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="ACGI8A_002053"
     CDS             6989..7174
                     /locus_tag="ACGI8A_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFH0180050.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="ACGI8A_002054"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="ACGI8A_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFH0180051.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="ACGI8A_002055"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="ACGI8A_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFH0180052.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="ACGI8A_002056"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="ACGI8A_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFH0180053.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="ACGI8A_002057"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="ACGI8A_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFH0180054.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="ACGI8A_002058"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="ACGI8A_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFH0180055.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="ACGI8A_002059"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="ACGI8A_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFH0180056.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="ACGI8A_002060"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="ACGI8A_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFH0180057.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="ACGI8A_002061"
     CDS             11117..11767
                     /locus_tag="ACGI8A_002061"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFH0180058.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="ACGI8A_002062"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="ACGI8A_002062"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFH0180059.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="ACGI8A_002063"
     CDS             12536..12805
                     /locus_tag="ACGI8A_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HFH0180060.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="ACGI8A_002064"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="ACGI8A_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFH0180061.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="ACGI8A_002065"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="ACGI8A_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFH0180062.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="ACGI8A_002066"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="ACGI8A_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFH0180063.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="ACGI8A_002067"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="ACGI8A_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFH0180064.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="ACGI8A_002068"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="ACGI8A_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFH0180065.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="ACGI8A_002069"
     CDS             14901..15848
                     /locus_tag="ACGI8A_002069"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFH0180066.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="ACGI8A_002070"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="ACGI8A_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFH0180067.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="ACGI8A_002071"
     CDS             16330..17163
                     /locus_tag="ACGI8A_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFH0180068.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="ACGI8A_002072"
     CDS             17151..18017
                     /locus_tag="ACGI8A_002072"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFH0180069.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="ACGI8A_002073"
     CDS             18011..18814
                     /locus_tag="ACGI8A_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFH0180070.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="ACGI8A_002074"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="ACGI8A_002074"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFH0180071.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="ACGI8A_002075"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="ACGI8A_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFH0180072.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="ACGI8A_002076"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="ACGI8A_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFH0180073.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8293 a         2449 c         4322 g         5700 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc tagatgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tatcccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtattaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tatagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt atttttataa
    20761 tatt
//
LOCUS       DAYATN010000059        20561 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.059, whole genome shotgun sequence.
ACCESSION   DAYATN010000059 DAYATN010000000
VERSION     DAYATN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.059"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            83..1369
                     /gene="grdG"
                     /locus_tag="ACGI8A_002077"
     CDS             83..1369
                     /gene="grdG"
                     /locus_tag="ACGI8A_002077"
                     /EC_number="1.21.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     alpha"
                     /protein_id="HFH0180074.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            1455..2762
                     /gene="grdF"
                     /locus_tag="ACGI8A_002078"
     CDS             1455..2762
                     /gene="grdF"
                     /locus_tag="ACGI8A_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_082007859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2502..2504,aa:Sec)
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     beta"
                     /protein_id="HFH0180075.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            3039..3770
                     /locus_tag="ACGI8A_002079"
     CDS             3039..3770
                     /locus_tag="ACGI8A_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFH0180076.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            3772..5130
                     /locus_tag="ACGI8A_002080"
     CDS             3772..5130
                     /locus_tag="ACGI8A_002080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0180077.1"
                     /translation="MINLFSSDLFLKYSFFFSFIVYRILMVIVQYYFLINFTNFNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            5220..5654
                     /locus_tag="ACGI8A_002081"
     CDS             5220..5654
                     /locus_tag="ACGI8A_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180078.1"
                     /translation="MKVGNINGQAPITNGLDSCQSEVQGLEQKKKALQDEIEKVRSDK
                     SIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEENNVSEVNNNLLERKKEPS
                     NNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRPI"
     gene            complement(6309..6533)
                     /locus_tag="ACGI8A_002082"
     CDS             complement(6309..6533)
                     /locus_tag="ACGI8A_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFH0180079.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(6630..7883)
                     /locus_tag="ACGI8A_002083"
     CDS             complement(6630..7883)
                     /locus_tag="ACGI8A_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFH0180080.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            8143..9132
                     /gene="add"
                     /locus_tag="ACGI8A_002084"
     CDS             8143..9132
                     /gene="add"
                     /locus_tag="ACGI8A_002084"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFH0180081.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            9204..9944
                     /locus_tag="ACGI8A_002085"
     CDS             9204..9944
                     /locus_tag="ACGI8A_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HFH0180082.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPSLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            10144..11295
                     /locus_tag="ACGI8A_002086"
     CDS             10144..11295
                     /locus_tag="ACGI8A_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFH0180083.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            11320..12075
                     /locus_tag="ACGI8A_002087"
     CDS             11320..12075
                     /locus_tag="ACGI8A_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HFH0180084.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            12267..14654
                     /locus_tag="ACGI8A_002088"
     CDS             12267..14654
                     /locus_tag="ACGI8A_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HFH0180085.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(14885..15118)
                     /locus_tag="ACGI8A_002089"
     CDS             complement(14885..15118)
                     /locus_tag="ACGI8A_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFH0180086.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            15407..15997
                     /locus_tag="ACGI8A_002090"
     CDS             15407..15997
                     /locus_tag="ACGI8A_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0180087.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            16111..16956
                     /locus_tag="ACGI8A_002091"
     CDS             16111..16956
                     /locus_tag="ACGI8A_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180088.1"
                     /translation="MIKVDDLSFSYTERDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            16953..17636
                     /locus_tag="ACGI8A_002092"
     CDS             16953..17636
                     /locus_tag="ACGI8A_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180089.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            17633..18349
                     /locus_tag="ACGI8A_002093"
     CDS             17633..18349
                     /locus_tag="ACGI8A_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180090.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            18446..18709
                     /locus_tag="ACGI8A_002094"
     CDS             18446..18709
                     /locus_tag="ACGI8A_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0180091.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            18681..19136
                     /gene="def"
                     /locus_tag="ACGI8A_002095"
     CDS             18681..19136
                     /gene="def"
                     /locus_tag="ACGI8A_002095"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFH0180092.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            19188..19649
                     /locus_tag="ACGI8A_002096"
     CDS             19188..19649
                     /locus_tag="ACGI8A_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HFH0180093.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            19699..>20561
                     /locus_tag="ACGI8A_002097"
     CDS             19699..>20561
                     /locus_tag="ACGI8A_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0180094.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSEGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEK"
BASE COUNT         8138 a         2169 c         3450 g         6804 t
ORIGIN      
        1 ttggcataga aaatgcttta tatattaata aacgtaataa atacttttaa ttgcgattaa
       61 atatattaag ggggattttc atatgaagct agagctaggg aatatattta taaaaaatgt
      121 tgaatttgga gaaaagacag aagtaaaaga tggagtacta tatattagta gcgaagaaat
      181 agaaaagata gtattagaag atgaaaggat tgtaagtgta aatgttgaac ttgcaagacc
      241 tggcgaatca ataagaatag ctccagttaa agatgtaata gagcctagag ttaaaattgg
      301 agatgaaagc aaaatatttc ctggaattat aaataaagtg aagactgtag gaagtggaag
      361 aacccatgta cttttaggag cttgtgttgt aacttgtgga aatatagttg gatttcaaga
      421 aggcgtaatt gatatgagtg gtcctactgc taaatataca cctttttcaa aaacaaataa
      481 tatatgtatt gttataaaag caaaggatgg tatagatact catgattatg aagaagcagc
      541 tagaattgca ggattaaaaa taggtgcata tgtaggtgag gcaggaagag aagttgagcc
      601 agatgaagtc attgtatatg agacaaagcc attattaaaa caagtaaaag aatatccaga
      661 cctgccaaca gtagcatatg ttcacatgtt acaatcacaa ggacttttac atgacactta
      721 ttactatggg gtggatgcta aacagatagt gcctacattt atgtatccaa ctgaaataat
      781 ggatggagct ataatatctg gaaactgtgt agcaccatgt gataaggtta caacattcca
      841 tcatttaaat aatccagtta tacatgactt atataaaaga catggaaaag atttaaactt
      901 tataggagtt attttgacta atgagaacgt attcttagtc gataaagaga gaagctctga
      961 tatggtagct aaattaatag aatttttagg agttgatgga gttctagtga cagaagaagg
     1021 ttatgggaat ccagatactg acctaatgat gaattgtaga aaatgtagtg aagttggagc
     1081 aaatgtagtt ttgataacgg atgaatttcc tggaaaagat ggcaagagtc aatctatagc
     1141 agacgctact aaggaagcag atgcagttgt ttcttgtggt caaggaaatc tagtggttca
     1201 tttcccagca atggaaaaaa ttataggaac tttagattat gttgaaatga tgataggtgg
     1261 gtataaagga tgcttaaatg aagacggaag catggatgca gagctacaaa ttataatagc
     1321 atcaactata gcaaatggct ataatcattt gacagcaaga tattattaaa taagtatttg
     1381 aatataaagt tctgttgatg taatcattgg tctaactgat gtaataacta taaagtgatt
     1441 tggaggatta agagatgagt aaaatcagag tggttcatta tataaacaat ttctttgctg
     1501 gtataggtgg agaggaaaaa gcagatattc cgccagaaaa aagggctggt gtagtaggac
     1561 ctggaatagc atttcagagt caatttaatg atgatgcaga gataatctca actgtaattt
     1621 gcggagatac gtattttggt gaaaatattg agagtgcaac tcaagaactt ttaaaaatga
     1681 taaaagaaga aagccctgat ttatttattg caggcccagc atttaatgca ggtagatatg
     1741 gagtagcttg tgggactatt tgtaaggcag ttgaagaaga gttaaatatt cctgtaatta
     1801 caggcatgta taaagaaaat ccaggtgtag atatgtttaa attagatttg catatagtgt
     1861 ctacaggaaa ttcagcagca agtcttagaa aggtagttcc aataatgacg aaactaggtc
     1921 ttaaattggt tagaggtgag gaaataggac ctcctgaaga ggaaggatat ataatgagag
     1981 gaataagaaa aaatttcttc catgaactta gagggtctga aagagcaatt gatatgttgg
     2041 ttaaaaagat gaaaggggaa agctttgaaa ctgagtatcc aatgcctgaa tttgatagag
     2101 ttacacctat gaaacctgtt aaagatttat ctaagataaa acttgcccta gtaacttctg
     2161 gagggatagt tccaattgat aatccagaca aaattgaatc ttcaagtgct actaaatatg
     2221 gaatttacga tttaacagat atggattcta tgtcaaataa agaatttaca actatacatg
     2281 gggggtatga cagagcatat gtccttgaaa atccaaattt agttgtacca ctagatgtag
     2341 ttagagagct tgaaaaagaa ggtgtaattg gagaattagt agattatttt ataactacta
     2401 caggaacagg aacgagtgtt ggcaattcta agagatttgg agaagagttt tctaagaaat
     2461 tgttagaaga tgatgtagat gctgtaattt taacttctac atgaggtact tgtactcgtt
     2521 gtggagcaac gatggtaaaa gagattgaga gagctggtat agcagttgtt catatatgta
     2581 cagtagtacc aatatcactt acaataggag ctaatagaat tgttccagca gtagggatac
     2641 catatccact tggaaatcct atgttaggag aagaggaaag taaaaaaatt agaaagaata
     2701 tagttttaaa agctttaaat gcactaacag aagatataga agaacaaaca gtatttgaat
     2761 aataaaaata caacacaaca ctgaaataaa aaaagatatc aaaaaataat tttttaaaat
     2821 tattttacga tatctttttt tattaggtaa attcgaaatt tgcatttttt taaattaaat
     2881 ttataaaagt gataaattag tttattataa tattgataca caaattcaaa ttagatatac
     2941 tatgaaaagc gagattatta cattttggaa caaatattca acatattaga tcatattaat
     3001 tgaacagaaa aaattaactt tatataataa ttagaataat gataagaaag aaggtgtata
     3061 tattgatttc aattgccatt tgtgatgatg aatcctatat gctaaatcat ttaaaggaaa
     3121 atatagaatt atacatgaag cagaagaatt tattatataa tatagagctt tttaataaag
     3181 tagaagattt actatgcaat catgaaaagt ttaatattgt atttttagat atacagatgg
     3241 aagagattaa cggaatgcag gcagctagaa aattaaggtc atatggtaat gagtgcttta
     3301 taattttcgt tacagtttta aatgaatttg tatttgatgc ttttgaagta gatgctataa
     3361 attatctgat aaaacctcta tctaatgaga aactatataa aactttggat aaaatagtta
     3421 caaaacttga tgataatgaa agtaattata tcacaataca aaaaggttat tcatattgga
     3481 aattaaaact tgctgatatt ttatattgtg aagttataaa aagaaagatt tatgttcatt
     3541 taaagaaaga gactgtagat tattatgaga ctattgaaaa tttagagaga agtttaccaa
     3601 caaatttttt taggtgtcat cgtagttata taatcaattt acaatatgta tatggatatg
     3661 aaaatggtaa tgcagttatg gaaagtggag atagaatacc tgtttctaga ctacgtcaac
     3721 aaatattttc aaaagttatg cttgagttca tgaaaaatag gaggatataa gatgattaat
     3781 ttattttctt ccgatttatt tttaaagtac agttttttct tctcttttat tgtatataga
     3841 attttaatgg taatagtgca atactatttt ctaatcaatt ttacaaattt taattcaaaa
     3901 ttttggtgtt ttattttata ttcgataatt tcaatttttt taatcattat aaatacatat
     3961 tttaatttat ataatttatt ttcttcacag attctatttt ttcttatgac ttttttattt
     4021 gtaaagtaca taataaaaca atctactgtt ttatctatag tatcaacttt gttagtatat
     4081 ttaattgaac aattagtata tggatttaca gctccattaa attacttgat atttactagt
     4141 gatataaata cagatattta taaatttgat ataaatatgg gactatcttc tttaattact
     4201 attataatag ctggatttat atattggtat agttctaaaa agtttaatat taaggtcatg
     4261 aactttgata aatatatagc aattttgatg attccattgt tgcttataat attatttatg
     4321 caatcatttg aatatagttc aaatataaat atagatacaa gtcttgggat tgtaaagctg
     4381 ctattaaata cttctataac tgaagaaatt caagcttttc ttttttctat aattggaact
     4441 atatgtgtat ttttttctct atttaccttt aagaaactaa ttcaagctct cgaagatgac
     4501 aaggagaggg caattatgaa tcaacagatt catgctcaaa aaaactatat tgaagaagcg
     4561 aaatcacgat tatcacaaac tatatccttt agacatgatt ttaataatca tttagctatt
     4621 gtcaatggat tgttgaaaaa agaccaaatt ttaaaagcac aagattattt aaataagctt
     4681 gaaaaagtaa caagtgacct atcattttct tgtaatacac agaatattgt tattgatgct
     4741 cttttcaaca ataaattaag tatagcaaaa caattaggca tagaggtaga ttgtgaagta
     4801 attatacccc aaagcactaa catgaacgat tttgacctat gtatagtgtt tgctaatgct
     4861 attgacaatg caattaaagc ttgtagagtt gtagataaac aaaataggta tttaaagctt
     4921 tccactaagt tagagggtgg attttttatg atagaaataa aaaatagcta taatccaata
     4981 aataattata caagtggctc aggtataggt cttttaaata taaaagaaat tgctaaaaaa
     5041 tatcaagggg ctataagtat agataagaca tcagactatt ttcaaataaa tgtattgcta
     5101 gttatttcat tacattgcgc tagtatttga gaacaatttc attgaaaata gtatttgtaa
     5161 gctatattat gtatttgaaa agagaaagtt tgatatatag aaatgaggag gttactaaaa
     5221 tgaaagttgg aaatataaat ggtcaagcac caattacaaa tggtttagat agttgtcaaa
     5281 gtgaggtaca aggcttagaa caaaagaaaa aagcattaca ggatgaaata gagaaagtaa
     5341 gaagtgataa aagtattgat ggtgaaacgg ccaaattaaa aatagaagct ttaaaaaaag
     5401 aaatggaaga gattgaaaat aaaattcaag agttaaaagg aaataaagga aaaaaaacaa
     5461 ataaagaaga aaataacgtt tcagaagtaa ataataatct attagagaga aaaaaagaac
     5521 cttctaataa tgaaaatgta aaacaattat atgatgtttt tgaaagaagc aatgtagacg
     5581 aacaatggga taatacttat cgtgttctcc aagatgataa aaacttaaaa gtaaaattta
     5641 atagacctat atagtttatt tataaaaata gttaatattt taattatcac cactttttag
     5701 aagaatacac actttatttt aaagacccac atatttattt acagttagca catttatatg
     5761 tggttttata actattattt acaagtctat attgataact tttacatagt atatgatgtt
     5821 taaatactta ttcaaattta tctatacata aacaaaaaaa gagattttaa aatctctttt
     5881 tagattgttg acttatggct ttttgattta ataaaatcaa gaagccgttt gaattttata
     5941 tgttggctga tacccttctg atattgaaac accatttttg tttccaaaat cgtttaaaat
     6001 taagaactta ttatgaatga ctatttcatt atgaacaata agcaaataac atgaatcttc
     6061 aatcaatgtt ttaacagtag cattaaatgc ttgcttatat agttctatcg tttgtttgaa
     6121 ctgcccatta aagtaaatat gagtcaataa cataactata tctacacaaa ttaatataaa
     6181 ttttttatac tgtacattat ctaactgttg attatctgta ttacaacatg attgaattta
     6241 aaaagccatc aacacataga atgttgatgg cctgaaaata gattttaaaa tctctttttt
     6301 attatatatt aattattttt taaatttata ttttttgcat cagattttct aatagctaag
     6361 tcaaaccctc taaatctaat cactataggg tcattaagag gagctatctt tttcacttca
     6421 acttcagtgc catctattag tcctaaggct agaagtctct tcataagttt ttcatttcca
     6481 gctatattgt ctataattcc cttttgtcca agttttaagt tgtaaacagt cattattaac
     6541 acctcctaat gaaaatcatt atcaaacttt atatttataa tatataacaa aaagagcaaa
     6601 ttgtcaatat ataacaaaaa ataaacatac taagttagta tgtttataaa aaatagtata
     6661 catgggatac taatagcatc tagcaaaaaa ccacttacta atggtacaac taaaaaggct
     6721 tttggagaat caccatattt ttgtgtcaaa gaactcatat ttgccagagc gttcggagta
     6781 gctcctattc catgtccaat caaaccactt attatgatgg cagaatcaaa atcttttcca
     6841 agtactctaa agcaaataaa tattgaaaat aggattataa agattacttg aaaagttact
     6901 attaagagaa taggaggtaa taatgtagat aattcaaata aatctatatt cataagtgat
     6961 aaagttaaaa aaattcccaa tgaaagttct tttaaaaaat ctataagttt aaaatttaat
     7021 tctagtatat ttatcttatc atttaaattt ctaaaaataa cagccgaaaa catacaagtt
     7081 acaacagagg gtaatagtat attaaacgtt atatagaagc atctacttat aatttctcct
     7141 gtatttatac aaataaatat gagaagaacc tgttccaaaa atacagatgg tgttattgtt
     7201 tcggagaagt tattattttt tatcctattc ttatttctaa aaaatttact tccaaatctc
     7261 tttagatttc tattatatct aggaatcgac aagtctatat ttgatgatgg ttttaattta
     7321 tatttattta ttagaaattt agctacagga catcccaata taccacccat aataagtcct
     7381 attgttgcag cagataatcc aaccccaact gctccttcta taccaagatt ttctattgta
     7441 agtccaaatg cagcagaata accatgacca ccttccatag atacatttcc acacataagc
     7501 cctaaaagag gttcaagttt tataatcttt gatattgtaa tagctaatat attttgacaa
     7561 aatgccaata ctccacacaa aatccaatat ctaaataata cactaccacc tttctttata
     7621 agatttatgc ttatttcaag tcctatagtt gtaaaaaaga aggttatgaa atttggcatt
     7681 aaatttccac ttatagatat atctgctata ttaaaatatt taaaaaataa atttagaaaa
     7741 caaaataata gacctcctat tactggtgca ggtatacaga aattatttaa aaagttaact
     7801 ttattcttca ataaattacc aattaaaaag aagaatatag atagtgtcaa ggtttgcact
     7861 atatcaaaac ttaaggtaat caaaataagt cctcctttgt aattcaaagt tttaattata
     7921 taacttaaat tcttatgtat tgaaatttta ttatcaaatg tatattaata aatgtatatt
     7981 aaaaagaatc agtagatata attagatagt cataaattaa attaaaatat tttaatgaga
     8041 tatgactttt ttagaattaa aaaccgtagt atattgatat aatagaaata agacttaaaa
     8101 atctgttgat agtattattt ataagtacag taggaggata aaatgtttga aaatttacct
     8161 aagatagatt tacattgcca cttagatgga agtgttagag tagaaaccat gcttgatata
     8221 gctattaaag agaaaataga tttaccttca aataatatgg atgaaataaa aaaattagca
     8281 aaggtttctt ttaattgtac ttcactagat gaatatttag aaaaatttga tttaccctta
     8341 aaagtaatgc aatctaagga aaatttaaaa agaattactt ttgaactttt ggaagatgca
     8401 agtagagaaa atgtaaaata tattgaaatt agatttgcac cattacttca cacccaaaaa
     8461 ggtatgagtg ttaaaaatat aattgagggt ataattgaag gaattagaga agctgaaagt
     8521 atttatgata taaaaggcaa tttaatatta ggttgtatga gaacaatgac ctcaaaagag
     8581 gcattattag taattgaaga agggaaatca tttgtaaata aaggtgttgt agcagtagac
     8641 ttgtgtggtc cagagaaaga gggattttgt aaggaatata aagatgtttt taaattagca
     8701 agagaatatg gatataaagt tacaatacat gcaggagaag ctgcaagtgg agaaaatgta
     8761 ttggatgcta taaatatttt aaaggcggat aggattggtc atggggtaaa aattaaagac
     8821 cataaaaagg cgtataattt agttaaagat aaaaaaatat tgcttgaatt atgtccaact
     8881 agtaatgtac aaactaagac agtagattct tatgaagcac atccatttta tactttttat
     8941 aaagataatt tacatgtaag tataaatact gataatagaa ctgtatcaga tataaattta
     9001 agttcagaac taaatgttat atttgatact tttaaactag gattagaaga ctataaaata
     9061 atttatagaa atgcagtaga agctagtttt gcagataaag aaactaaaga gtatttgaat
     9121 agtttaattt aatagataaa aatatttaaa ctttaaatta agctattaaa ttgataaaaa
     9181 tacattgtat taggagaaaa aatgtgaaag attatgaaga tgttgtttat attaaactag
     9241 tcaaaaaaga acttgaagaa tggaagaaaa atatattaaa gaaaccttct atatttgaca
     9301 aagcatcaaa gggtatacag aataaattta atggagtatt gccagataaa tatcatgaaa
     9361 taattacttc ttctataaaa gtaatgacaa aaaccgtact gtttggatat aaatatgcta
     9421 caaagccatc acttagaaat atgtcattag aagaaagaga aaaattagtt ttagaaaaag
     9481 ctaaaactta caagactaca gcaatgattg aaggggcagg aacaggggca ggaggaatcc
     9541 tcttaggttt atctgatttt cctttgcttt taggtattaa agtgaaattt ttatatgatg
     9601 tagcttcaat atatggatat gatgttaaag attatagaga aaggatttat atattaaata
     9661 tattacagtt ggctttttct agtcaacagc atataaacaa aacttttaaa ttgatgaata
     9721 actgggatga atatgtaaat agtctaccag aagacatcaa tgattttgat tggagaacat
     9781 ttcaacaaga gtatagggat tatttagatt tagctaagtt tttacaacta atgcctggaa
     9841 taggagcatt tgtaggattt tatgtgaatg gaaaattaat tgataaatta tgtgaaactg
     9901 ctgtgtctgc ttatagattg aggattcgag agcttaagtt ataagagaaa ttataacata
     9961 aaaaattagt tatacaatat atctatatgt atctaatttt gcattgataa aaacaattat
    10021 ggataaaagg taaagcatga tagaatctaa tgtagaggtt tgtattttaa tggaaaaatt
    10081 tagtttttat tgcttaagat acaaattgaa ttacgaagag aagtaattgg gaggaatatt
    10141 ttcatgacaa atttgccaaa acaatttgat agcttcaatg aagctagaca aaaaggattt
    10201 ataaatgcaa aggaacttaa ggaaagtggg aaaaagatgg ttggagtttt ctgtacattt
    10261 actccagttg aaattccaat ggcagctgga gcaactgtag taggtgtatg cggcgttagt
    10321 gaagagccaa tacctgatgc agaaagggtg cttccaagaa atctttgtcc tttaattaaa
    10381 tcaagctatg gacatgcaat aacagatacc tgtccttatt tctatttttc tgatttgttg
    10441 ataggagaaa ccacttgtga tggtaagaaa aaaatgtatg aagaacttgc aaaagttaag
    10501 ccaacatatg taatgcattt gccaaatact tcaaaaggag aatttgccta taaactttgg
    10561 aaagatgaaa tgataagatt aaaagaagag gttgagaaat ctttaggtgt aactataact
    10621 gaagaagata taagaactgc tataaaagat aaaaatgaag aaagagaact tttaaaagag
    10681 ttttatgcac ttggtaaatt acaaccatct gcacttacag gccttgagtt acataatgtt
    10741 ttatatcaag caggttttaa atttgaccgt gcagaactta aacattcttt aagaaaagtt
    10801 atagatgata tgaaagaaag atatgaaaaa ggtgaatgtc ctgttcaaaa agataagcca
    10861 agaatattga taacaggttc tcctattggt ggaataagcg aaaaaatagt taaaacttta
    10921 gaagatgcag gggcatcagt cgttgcatat gaactttgtg gagctattag atcaaacgat
    10981 ttattggttg atgaagaaat agaagatgta tatgatgctt taacacaaaa atatataaat
    11041 ataggttgtt cttgtatgat gaataatgac aatcgtattg aattattaga tagaataata
    11101 gatgaatata atgtagatgc tgtaatagat gtagttttac aagcatgtca tacatttaat
    11161 atagaaagtt atagaataag agaatttgtt acaaaagaaa aaaataagcc atttatgtct
    11221 cttgaaactg attattcaaa atcagatact gaacaattaa gaactagatt tgaagcgttt
    11281 gtagagatgt tgtaggaata aataaaagta ggaggaacta tgtttagtat aggagttgac
    11341 tcagggtcag tggcaacaaa gggtgtgcta tttgatggtg aaaaaattat taagaaaata
    11401 attataccaa ctggatggag ccctaaaagt acatctaaac aggtctatga gttgctaagt
    11461 agtgaaattg acaaaaaaga tataaaaaag gtggtaggta caggttatgg aaggggagta
    11521 atggattttg ctgataagaa agtaacagaa ataacttgtc atactagagg tatttacttt
    11581 ttaaacaaga acataaggac tattttagat gtaggtggtc aagatagtaa agttataaat
    11641 ttagatagag atggaaatgt atttaacttt ataatgaatg ataagtgtgc tgcaggaact
    11701 ggtcgatttt tagagattac ttctaacctt ttaggaagcg atattgaaag tatagataca
    11761 ttagctaaag gatatgaacc agttaatata tctagtatgt gtacagtttt tgctgagtct
    11821 gaaattgtaa gtcttctagc tcaaaacatt tctactggag aggtagcagc agggatactt
    11881 aaatctattg caaataaatc cacctcaatg ttagcaagag gtgaggtaat agatgaagtt
    11941 gcatttactg gagggcttgc aaaaagtaaa gaactagtaa aaatgataga agaaatactg
    12001 ggtaaaaaaa tttttattgc agaagatact caaataatag gagctttagg agctgctgta
    12061 ataggattta gataaaaaca tataaaagga aaatgagaag ctgaatttat tataaataaa
    12121 ttcagttttt tattttattt tatttattta taaaggaaaa ctttggtata attgatttaa
    12181 atgattgtat atttgtaaat acttgatatt tagtcatata taacattata attttaaaaa
    12241 ttagctcaaa ataaggggga gaaagcgtga aaaaatttac ttcaaaaaaa gtaacaaggt
    12301 ttgttgcgct gtttttaata tctattttag taatgtcaac aattcctgta tcagctgaca
    12361 atagcaatac attgaatcag ataaaaaaag cagaatacag tgagacgtat aaacaatatc
    12421 taaatgatgc taaaaatgga cgtacagaaa aatataatgg gattatacca aatccatatc
    12481 agttggaagg tactcaaatc caaagtaaag gaagaacagc tccaacatct tatgacccta
    12541 gaaaattagg tcttatgaca tcaataaaaa atcaagagga tttaggaatc tgttgggatt
    12601 ttgctgcaat ggcaacctta gaaagtttct taaagttaaa taattatgga gactatgatt
    12661 tatcagaaga acacttaaga tggtgggctt cagatggtga atatggatgg tctgtaaatg
    12721 atatgaatgg tgctttaaat tatgaagcta tgggatattt aacatcttgg tcaggtccta
    12781 aactagaaaa agatattcca tacaatggta gagtatctaa agcacaagga gcaaaaaaac
    12841 caactaatat gaatacagca cctactgttt ttaatgtgac agatgctgtg tgtgtaagta
    12901 atgacataaa ttctacaaaa aatgctatat tacagtatgg agcagtaaca tctggttatt
    12961 atgaagatat taaatatcaa agtgatgacc aaaattcata ttattgtcca acaaaaagtt
    13021 ttaatacaaa tcatgctatt tcaattgtag gttgggatga taactattct aaagataatt
    13081 ttaatgcaaa tattagacca tcaaaaaatg gtgcttggtt aataaaaaat agctggggag
    13141 attataattc tgagggtgga tatttctgga tatcttatga agacaagaca cttatgtctg
    13201 atatagataa tttttcaatc aaaggtggta aaaaaccaaa tgatgatgag aagatgtatc
    13261 aacatgacta tgcaagtatt gtacctttaa tttcaaaaaa gataacagct gcaaatgtat
    13321 ttgatttcaa tagaggcgat gaaactctaa aatctgtaat gttcttaact gaaagtatag
    13381 gagctaagta tgaagtatat tatgctccag tagtaaatgg tatacctcaa gaaaacaata
    13441 tgaaaaaatt aaaagaaggt actgcccaat attcaggata cataacagtt ccaattgatt
    13501 cttttgatat accagaagga aagggtgcaa ttgtagtcag tatagagggg aaaaatggag
    13561 agtcaactat aggttctgag tcaaatgtac caggatatga tacatttaaa gcaaaagcta
    13621 atttaggtga aagttatatc atagataata ctggaaaatt ctttgatata aatagagata
    13681 gtaacttcta tccgtgtaat tttactataa aagctgttac agaaaagtct tctggtgaga
    13741 gtatcccaaa tgagtcttta attggtagtg atagatatga aactgctatc aaagttagtc
    13801 aaaatggttt caattcttca gaaaatgtag ttttagtaaa tggcagttca atagtagatg
    13861 cattggcagc aactcctttt acatctgcta taaattcacc aatattattg acacaaaaag
    13921 aagctttaaa ttcaaaaact aaagctgaga tacagagatt aggtgctaag aaagtatacc
    13981 taataggtgg agaaaattct ataagtaaag aaattgaaca acaattaaaa agcttaaata
    14041 tatcgataga gagaatatca ggaagtgata gatacaagac aagtttatta ttagctcaaa
    14101 agttaaatgg tataaaaaat gtatctcaaa tagcagttgt aaatggagtg aaaggattag
    14161 ctgatgcaat tagtgtaggc gctgcagcag cagaaaataa cataccaatc atacttgcaa
    14221 atgaaaagag cgaattgcaa ggagctgatg aatttctaaa ttcattaaat atagagaaat
    14281 cttatattat tggaggaaca gcatccctat caaacaatct agaaagtaag ttaaaaaatc
    14341 caactagatt atctggtagt agtagagacg aaactaattc aaagataata gataattttt
    14401 ataaaaaaga tactttaaaa aatgcatttg tagttaaaaa tggaatcaag aatcaaaatg
    14461 atttaataga tggattggca gttggtcctt taggagcaaa aactgggtca ccagtaattt
    14521 tagttggtga taaattagct gatagccaaa aagaagttct taaaaataag actttagaaa
    14581 aagtaactca agttggtgga ggagcaaata aaaatgcatt taaagagtta gtaaaattaa
    14641 aaagtgctaa atagagtact taagtattaa catatattag tgtgtattag taacttatct
    14701 taataaaagc tgaaaataat tacttcaaat tattgataaa taagataatc aatgattgta
    14761 aagttttgtt ttcagcttta aatttatgta gtataagttt aaaaacataa aaaagggtta
    14821 ctttatatag aaatatagaa aatcttacat tttatatata aataacccat atttaattaa
    14881 ctttttatag tgttctaata gatattattg aacttaatgg taaatataat gtgtaagttt
    14941 catttaaagt tggattagat tcatctatat atcttgagca aagtgtaatt gataagctat
    15001 ctgcttctat aatttcacca tatagaggac aactgctaaa gtaagaatgt acttcaacat
    15061 atttatttaa gttttcttga acataatcgt aaaaacggtt ttttgtagta gtagccataa
    15121 aatcaactcc tttatttttt attaaagttg aaacttttaa aaagataaat atgattactt
    15181 ctattagaaa ctaagttaaa aaataagcaa atagatatga aaattagaat aatatattat
    15241 ttatatctta attataacac tagataataa aaaatgctat ataaaattca gaattttcga
    15301 aagtttttaa gcttattttt aaatatacta ataatactat tgacattaga tagaaaaagt
    15361 attatattga ctatataaat tattttagtc aaactggagg gaaaagatgc ctaaatcgta
    15421 ttctgataaa gaaagagagt atataataaa aagacttaaa gaagaagcaa gattgtgtat
    15481 ggagcaatat ggtattagaa aaactactgt tgatgagctt gtaaaacggg taaaaatacc
    15541 aaaaggaact ttttatctat tttttcaatc aaaagaattg ctcttttttg aggtattaag
    15601 agatatacat gactctatac aaaaagaaat cttatatgag ataaatagag tagatgaaag
    15661 tattacatgt gaacaattga cagatatatt tatgaaattc tataggatgg ttgatagtac
    15721 ttcaatttta aatcttatga taaatggaga atttgagata ttggtgagaa agttaccaga
    15781 ttctataatt gaagaacact ttagacatga tgactttgaa attggagaga ttatatcttg
    15841 cataccaaat gcaaaaaata aagacataga aagctttagt ggagcattta gagcagtttt
    15901 tcttaccatg ctatataagc gcgaggttgg aagtaattgt tttgaagatg ctttaagatt
    15961 aatgataaac ggattagtta tacaacttat ggaatagaat ttaaattttg tttgctaata
    16021 acttttatta aaaattatta acaaacaaaa aaattatatt ttattgacca tatagtcaaa
    16081 atagtcaaaa atttgaaagg gtgatattga atgattaaag ttgatgattt atcatttagt
    16141 tatacagaga gagatttttt acaaaatatt aactttggag taggaaaagg ggaaatacta
    16201 ggatttttag gaccatcagg tgcaggaaaa tctacattac aaaagattct aattggaatg
    16261 ataactaatt atggaggaag tgtcattgta aatggagtag agagtaaaag acactcaaat
    16321 aaattttatg aaaatatagg agttgatttt gaatttccta gtttatatga aaagttgact
    16381 gcaatagaaa atttaaaata ttttgggtct ctatattcta aaaaactact atctattgat
    16441 gagttattaa aatcagtagg actagaaaat gaagcaaata aaagggtatc tgagtattca
    16501 aaaggtatga aatctcgatt aaattttata aaagctttat tacataatcc agatatttta
    16561 tttttagatg aaccaacaag tggtcttgac ccatcaaata gcaaggttat gaaagatata
    16621 attttatcag aaaaatctaa aggtaaaact attattttaa caactcataa catgttagat
    16681 gcaacagagc tttgtgacag agtagcgttc attgtaaatg gtaaaatatc tgctttagat
    16741 acaccacata atcttattat gtcaaaagga gctattaaag ttagatatac ttactttgat
    16801 aatggagaaa aaacttctga atgttttctg aacaatactg ctaatgataa aaatttgaat
    16861 atgcttattg agaaaaataa acttttatca atacatagta gtgaaccaac attaaatgat
    16921 atttttattg agataacagg gaggaattta caatgaggct aaaaaatctt attttaggag
    16981 atataaagtt tcaatttaag tatggttttt attttttata tcttttttta agtattatat
    17041 atatttgtat tatcaatgta tttccaactt ttatgagaga aaaaatagct ataattatga
    17101 tttattctga cccagcagca atgggattgt tttttatggg agctattgta cttcttgaga
    17161 aaagtcaaag ggttttaaat tccttagctg tatctccagt aaaagtatct gagtatattt
    17221 tatcaaaggt aatttcatta ggagtaatat ctagtattgt agctatgttt attgctatta
    17281 cattaaattt agataatatt ataatatcaa ctataggtac ctttttttct tctattatat
    17341 tttctttgct tggattgata cttgcaagta aagcatctag tttgaatcag tttattgttt
    17401 taagtattcc tattgagatt atatgcttta ttccaccaat actcaatgta cttcttgaca
    17461 caaaatcata tgcaaatttg taccctttta atatatgtat ttctcttatt agtggagata
    17521 aaaattttat aatgattaat attttaattt taatatcaat tataataata atttatttta
    17581 taacttatta ttttatttgt agcagttgga aaaaggtagg tggagtaaaa ctatgataaa
    17641 gattataaat gcgtttaaac aagagtttga acaaattaag agagatgcta tgctttttat
    17701 agtatgtgtt tctccaatat tatgtggagt atttataaaa tttggaatac cattaataca
    17761 gaatatatca cttaacaagt tttattatca attaaattta gagccgtatt tcttgatgtt
    17821 tgatttactt ttagctttta ttacaccttt tatgttcttt tttgcttcta caatggtcat
    17881 attgggagaa attgatgaca gcatttcaag atatttaata gtaactccgc ttggtaaaac
    17941 tggttatctt atttcacgat ttggaatacc aggtatctta gcatttatta ttactatgat
    18001 tctacttatc tttttttctc taactaaaat ttctttcttg ttaaacttat caatttcttt
    18061 attagtttta ttacaaggaa ttattatatc aatattagtg atttctttat catcaaataa
    18121 actagaagga atggtaatta cgaaattctc aggtgttttt atgatgggta ttttagcacc
    18181 attttttatt ttaaataaag cacaatatat tctgttcttc ttaccaactt tttggatatc
    18241 gaaggcattt aaggaagata attatgtcta tatgtttatt tcaattgtta tatcattaat
    18301 atggatttta ttattattta aaaagtttag taaaaaaata gttaaataag aaaatttaat
    18361 atataaatgt aaggttgact tgacagtaat gttgaaaaat gttacattta agatgtaaag
    18421 ttaaccttac atgaatggag caaatatgaa aaataaaata aagatattaa gagaaaaatt
    18481 aggattgaca caagaacaac tagggagatt agttggaaca tctagacagg ctataaatgc
    18541 tatagaaact ggtaaaaatg aaccttcaat atggttggca tatgatattt caagaatttt
    18601 taatgaacca attgaaagtg tatttttatt tgaagaaagt gaaagaaaat caagagcaca
    18661 gataagtagg ggagaatatt atggctataa gaaagataag aacatttgat gatgaaattt
    18721 taagaaaaaa gagtaagtat gtagaaaacg tagataataa gataagagaa atattaaatg
    18781 acatggcaga gaccatgtac aatactccaa atggaggagg attagctgca tgccaagtag
    18841 gagtattaaa gcgattagtt gttatagatt taggagaagg acttattaag cttgttaatc
    18901 ctgaaattat taaacaagaa ggggaacaaa ttgttgttga aggatgttta agttttcctg
    18961 aagtatgggg taaattaaaa agaccaaaaa aagttacagt gcaagctcta aacgaatacg
    19021 gtgagaaaat agaaattaaa ggttcaggtt ttatggcaaa atgtctttgt catgagatag
    19081 accatttaaa tggtatagtt tttacagata aaattattga acatgtaaaa ttgtaagata
    19141 gattggttat tgaatttata aaatatttta tagtaaggag ttaaattatg agattatggc
    19201 ataaggattt aattgatgta ttacctaaaa atcaattagt aagtcaatgg agagagctac
    19261 ttgctataaa aggttctata gataaaaaag gtactccaaa ccatctatta gtgaataaag
    19321 tattgaatta tagtatagat gaatttaaat tttatacaaa aattgtacat gatgaaatgt
    19381 taaaaagaaa ttataaacct aatgaactta aatatacaag tatattaaaa tggaaaaata
    19441 gaaattttgc aaatgatata tctaatgaac atagtttaaa tttagaaaat ttatatgatg
    19501 attggcataa taaaatgtat ttaaagcaat gcttatataa tttagaagaa aaagcaacat
    19561 gtggaggaat accaataaat gaatggaata tattactttg taaatatagt aaagattatg
    19621 aattgtggtc tggaaatatc atgttttaat tactatagat taacattata gtattattgt
    19681 atataaagag gtgattattt gtataataaa ataaaatata ttccaataaa ttgtgaaata
    19741 gcttgtaata aattaaaaag gagtatgcca tataaatggg atttaaatat ttatcgtgga
    19801 tgtgagcatg gatgtaaata ttgttatgct atttactctc ataagtacat caactccaat
    19861 aattattttg aagagattta tgtaaaaaca aatatagtag aaaaacttga gagacaactt
    19921 aaaagcagta aatggaagaa agaagtaatc aatataggag gagttacaga tagttatcaa
    19981 ccaatagaag ctgactataa aattatgcca gaaatattaa acctgctaat aaaatacaag
    20041 acaccagcta ttatttctac aaagtcagat ttaattttaa gagattacga tttaattgat
    20101 aagctatcta ggattacata tatcaatgta gcatctacaa ttaccacagt aaatgaagat
    20161 acacaaaggc ttattgagcc aaatggtgtg gattcaatga gacgatttaa aatgttaaaa
    20221 gaatttagaa aaactaacgc atcagttggt ctacatatta tgccaattat accatatatt
    20281 acagatgatt ttgataatat aaattcgcta tttagacatg caaaagaatg taatgtacat
    20341 tatgttttac ctgggacttt atacttaagg ggtataacta gaggtgtttt ttttgacttt
    20401 gttaaaaaag agtttcctga attatttgat aaattatcaa tactatatag tgaaggttca
    20461 cttaataggg agtataaaaa tcaattgtat aaaatggtta atgaacttag atataaatat
    20521 tctctatcaa gcagttactc taaaataatg aaggaaaagt t
//
LOCUS       DAYATN010000060        20551 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.060, whole genome shotgun sequence.
ACCESSION   DAYATN010000060 DAYATN010000000
VERSION     DAYATN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.060"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            69..3017
                     /locus_tag="ACGI8A_002098"
     CDS             69..3017
                     /locus_tag="ACGI8A_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0180095.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            2995..3459
                     /locus_tag="ACGI8A_002099"
     CDS             2995..3459
                     /locus_tag="ACGI8A_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0180096.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3572..3874
                     /locus_tag="ACGI8A_002100"
     CDS             3572..3874
                     /locus_tag="ACGI8A_002100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0180097.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3951..5300
                     /locus_tag="ACGI8A_002101"
     CDS             3951..5300
                     /locus_tag="ACGI8A_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFH0180098.1"
                     /translation="MEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGLTVGVG
                     FIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALIIPIGL
                     VVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIVLVMAD
                     LTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQKKFGL
                     FGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLPVSEAA
                     QEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVLPFADL
                     AVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFKMPEGA
                     TMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKLSIEE"
     gene            5335..5808
                     /locus_tag="ACGI8A_002102"
     CDS             5335..5808
                     /locus_tag="ACGI8A_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180099.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            5980..6630
                     /locus_tag="ACGI8A_002103"
     CDS             5980..6630
                     /locus_tag="ACGI8A_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFH0180100.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6804..7736
                     /locus_tag="ACGI8A_002104"
     CDS             6804..7736
                     /locus_tag="ACGI8A_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFH0180101.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8010..8723
                     /gene="ispD"
                     /locus_tag="ACGI8A_002105"
     CDS             8010..8723
                     /gene="ispD"
                     /locus_tag="ACGI8A_002105"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFH0180102.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8720..9205
                     /gene="ispF"
                     /locus_tag="ACGI8A_002106"
     CDS             8720..9205
                     /gene="ispF"
                     /locus_tag="ACGI8A_002106"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFH0180103.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9300..9508
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9572..11287
                     /locus_tag="ACGI8A_002107"
     CDS             9572..11287
                     /locus_tag="ACGI8A_002107"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFH0180104.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11409..12854
                     /gene="proS"
                     /locus_tag="ACGI8A_002108"
     CDS             11409..12854
                     /gene="proS"
                     /locus_tag="ACGI8A_002108"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFH0180105.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13292..14773
                     /gene="gltX"
                     /locus_tag="ACGI8A_002109"
     CDS             13292..14773
                     /gene="gltX"
                     /locus_tag="ACGI8A_002109"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFH0180106.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            14994..15149
                     /locus_tag="ACGI8A_002110"
     CDS             14994..15149
                     /locus_tag="ACGI8A_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180107.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15294..16691
                     /gene="cysS"
                     /locus_tag="ACGI8A_002111"
     CDS             15294..16691
                     /gene="cysS"
                     /locus_tag="ACGI8A_002111"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFH0180108.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16694..17089
                     /locus_tag="ACGI8A_002112"
     CDS             16694..17089
                     /locus_tag="ACGI8A_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HFH0180109.1"
                     /translation="MEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRKVNDLHK
                     LAIKYVKAKAQATIIHEVEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKHATGFEA
                     LVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17195..17953
                     /gene="thyX"
                     /locus_tag="ACGI8A_002113"
     CDS             17195..17953
                     /gene="thyX"
                     /locus_tag="ACGI8A_002113"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HFH0180110.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            17966..18703
                     /gene="rlmB"
                     /locus_tag="ACGI8A_002114"
     CDS             17966..18703
                     /gene="rlmB"
                     /locus_tag="ACGI8A_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFH0180111.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            18706..19233
                     /locus_tag="ACGI8A_002115"
     CDS             18706..19233
                     /locus_tag="ACGI8A_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFH0180112.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            19298..19945
                     /gene="sigH"
                     /locus_tag="ACGI8A_002116"
     CDS             19298..19945
                     /gene="sigH"
                     /locus_tag="ACGI8A_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HFH0180113.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            20019..>20551
                     /locus_tag="ACGI8A_002117"
     CDS             20019..>20551
                     /locus_tag="ACGI8A_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFH0180114.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT         8499 a         2150 c         3850 g         6052 t
ORIGIN      
        1 taagtacaaa aaaaacaata tatggcatat aagttgctat ataaataata aggagggaac
       61 aattataaat gaggcaaaga ataaaaaact taattgaaaa tgaagataag aaaaatcctt
      121 taacagatga agcgatttca agtaggttaa atattcgaag agaagatgtt acctttttga
      181 gaaatgagtt aaaaatagaa gattcaagac aaagaagaaa agttgtttta attaaagcta
      241 taaaagatat tttaaaagaa gaaaaaacta taaataaaaa tgatataaca agaagattaa
      301 acagcattgg gtttacagta tcaagattta cagtcataca atacttaaaa gagattcaag
      361 attctggtga cctaaaactt gataaagaga gtgatgataa aaaagaagaa ataatcaata
      421 atagagaaaa taaagatata gcatttgata aactcatagg tagtagtggt agcttaagta
      481 caattataaa actagccaaa gcagcaattc tttatccacc tcatggtctt catactatta
      541 ttatggggcc aactggtgtt ggaaagagtg agttagcaga gtgtatgtat aaatttgcag
      601 tagaatcatc cagattccct aagaatgcac catttatagt ttttaatgca gcagattatt
      661 cagagaatcc acagcttttg ctatcacagt tatttggaca cgttaaaggt gcttttacag
      721 gagctgatga gaacaaagaa ggattagtat caaaggcaga tggaggaata ttgtttatag
      781 atgaaataca tagattacct catgaaggtc aagaaatact atttcaactg attgataaag
      841 gtatggtcag aaagttagga gaaacaagac taacacataa aatagatgta atgattattt
      901 cagcaacttc tgagccaata gattctcatt tacttaatac atttaaaaga cgtatacctg
      961 taacaattga aataccagaa ttagttgcaa gaccattaaa tgagaggttt gacattataa
     1021 ataatttctt cttaatagaa gctcaaagaa tgggagcaaa catacatata aagtcagatg
     1081 tattaaaagc tcttatgctt tacgattgta taggtaatgt aggtcaactt agaagtgata
     1141 ttcaagtagc ttgtgctagg ggactattaa accaacttac aaacaaacta aaagaagtaa
     1201 atataagcat atcagatgta cctggatatg taaaacaagg tcttataaaa atcagaaatt
     1261 gtagaggaaa aatagaaaat tatgtagatg gggatttaat tatagactca aatcttcaag
     1321 gtgagataga aaagaaaaat gaagatatat atacttttcc agatgaaata tacaaactta
     1381 ctgaaagaag acatattgat ttattggaac aaggtttaga ttttgatgtt ataaatagaa
     1441 tcattggagg agaattagaa agttgcattc aaaaatacat aaaacaagtt aaaaaagttt
     1501 ctattagtaa caatcttcca gatataagtc caattgttgg aaaatatatc gtagagctaa
     1561 cttatgaagt aatcaaaata gcatcacaat acctaagttc actagaaccg aatctacatt
     1621 tatgtttagc agttcaccta aaagcagctt ttgagagatt aaaagaagga aaagtaatat
     1681 caaatgctca tttgaaagaa ataataggaa attatccact agaatattct gtagcagaaa
     1741 aggtagctca atatttaaag gaaagatata acataaactt accaaaagaa gaaataggta
     1801 taatagcaat atattttaaa atgagtagta gaagagatat gtctgaacaa aaaaagatag
     1861 gtgtacttct tatggctcac ggaagagtag catcttctgt atgtgaggtt acaaataaac
     1921 tattaggaat taatcacgca aggtatttag atatgccatt tgagaagaaa ccggaagaaa
     1981 tgattgaaga agctactaca ataataaaag atattgatga aggtagaggt gtgattatat
     2041 tagtagatat gggttcactt tcatattttg gagatattat ttctggaaag actggaatta
     2101 atataaggac aatatctaga tttgatacat tgctttctat agaggttata cgaagagcag
     2161 ttttaccaga tttaacaata gacgagatag ttgatgaagt taaaggtata gagccacaag
     2221 taaaagaaaa ggttagtaaa tgtaataata caaaagtaga ttttagaaaa cctataattt
     2281 taacattatg tattactgga ttagggggag cttgtaagat aaaaaaaatc ctagaggaaa
     2341 atattaaaag tataactgaa aaggttgata tagaacctat aggtatgatt gaatatccaa
     2401 gtcttgattt agagataaaa caaataagca atgataaaaa tgtaatcgcc atagttggaa
     2461 caattgaccc tcagatagca aatatccctt tttttagtct agtagacata aatgataaag
     2521 caaagttaaa tcaaataaat aacctaattg atacaaatca aattggtgaa acaaataaaa
     2581 aatatgaatt atcagattta atacataaag aagatatatt tgtggattta caggttaaat
     2641 caaaagaaga agttctaaaa tatatgagta aaaaacttag agagaaaggt tatgtgacaa
     2701 aaaatttcta taaaaaagta gtggaaagag ataacttatt tccaactgaa ttatcagatg
     2761 aaatagcaat acctcacaca gatggaatag atgtaattag gccagctata agtatagcca
     2821 tcttaaagaa gcctattgtg tggagtaaaa gtaaagtaaa aataatactt ttattagctg
     2881 tagaccaaaa gtgttttaat ccattaagca ctctactctc atttatagaa acagatgaat
     2941 ttaagaagat aaaaaacatt aaagatagag attttattag ggagatgata ttagatggag
     3001 tcaatgaaaa taattagtag caatttaatt tttaaaaata ttgaagtgtc taacaatgaa
     3061 gatgcattaa agtttttggg acaacgtttg tttgacgaac agtatgtaaa agaaagctac
     3121 atacaagctg ttgtagcaag agaaaaaaag tatgctacag ggttaccaac agaaatatac
     3181 ggagtggcaa taccacatac agatatagta catgtaaatg agccaggaat agcaattgga
     3241 atactgaata aacctgtaaa atttataatg atgggtacag acgatactga aattgatgta
     3301 aaagttgtat ttatgttagc agtgaaagag ccacaagaac aattgcaact attagaaaaa
     3361 cttatgacta tatttcaaga taagaatata ctaaataata ttgttgactt aagtgaagaa
     3421 agtgtgagtg atttattaaa ctctaaattg aaaaattaaa catgtaaagt atattaaaaa
     3481 tgtaaaatta aacatgcaaa gtatgttaaa aaatataaaa agattattaa agatttctaa
     3541 ctatagatat ctttaaaata aaataatttc aatgggggtg actaatgtgg ctaaaaagat
     3601 attagttgca tgtggaacag gagtatgtac ttcaacaatt gctataaaca aattaaagaa
     3661 agcacttcaa gacattggta aattggatat ggttaatatt acacaatgta aagtagtaga
     3721 agttgcatca aaagcacctg attatgattt aataatttgt actacacaag tatctagtag
     3781 tataaaaaca ccagtgataa atggattacc ttttttaact ggtgtcggaa tggataaatt
     3841 aatcaatgat gtattagaag agttagaatt ataattaaaa aaattaagga acatggtctc
     3901 taaaatcata agttttagag acttaaaatt aaataatgga gggtctttat atggagatat
     3961 taaatttcat tgttggtcta ggtgcacaag taatgatgcc aataataata tgtatttttg
     4021 gtttgatatt aggtacaaaa ctaggaaaat ctttaagagc tggtttaact gtgggagttg
     4081 ggtttatagg tcttaacact attatagctt tgctaacaga taatttaggt ccagctacac
     4141 aacaaatggt taagaattta gggctaagtt tatcaataat agatgtagga tggccagcag
     4201 catcagctat agcttttgca tcaacagtag gggctttaat tattccaata ggtcttgtgg
     4261 taaatatagt tatgctaatt accaatacta ctcaaactgt tgatgtagac atatgggatt
     4321 actggcattt tgcttttaca ggagctttag tggcaggggc tactcaaagt gtaatgtggg
     4381 gagtatttgc agcagtagca aatatggtaa ttgtattggt aatggcagat ttaactgctc
     4441 caggtataga ggaatattta ggtatgccag gtatatctct tcctcatgga tttacacagg
     4501 catttgttcc aattgctata gtagtaaata aattattaga cttaatacca ggcataaata
     4561 agattgagat aaatgctgat acattgcaga aaaaatttgg attatttgga gaacctttaa
     4621 taatggggtc tgtaataggt gtaataattg gtattgctgc gaaatatgat ataaaaggaa
     4681 tattacaatt aggtgtaact atgggagctg tattaatatt aattccaaag atggcagcat
     4741 tattaatgga aggattatta ccagtatcag aagctgcaca ggaatttatt gagaagagat
     4801 ttaaaaacag aggcaagatt tatataggct tagattcagc agtaggaata ggtcatcctg
     4861 ttacattatc agttgcacta gtgttggttc ctttgactat acttatagca gctatattgc
     4921 caggaaataa agttttacca tttgcagatt tagcagtaat tccatttgca cttgtattaa
     4981 tcgtaccaat tacaaaggga aatgtgttta gaacattaat aataggaata attataatta
     5041 cttctggatt attaatcgca actaacttag ctccattatt tacccaaatg gctttaaatg
     5101 ctagttttaa aatgcctgaa ggagcaacta tgatttcaag tatttgtgat ggtgcgaatc
     5161 cattaagttg ggtatttgta aaagttatga actataaagt tattggagga gtagtttttg
     5221 gagttatagc tttaggaatg gctatttaca ataggaatag aataataaat gaaaataaaa
     5281 agctatctat agaagaataa ggttaaaaca attttattaa ggagtgtata ggttatgaaa
     5341 aaatataaat atgattctaa aagatactta ctcagtgtta caataccagg attttttatg
     5401 attttaatat tgatttatgc actgtttaat aactatacaa attttaattt gaatatatat
     5461 agtttgttaa taattgtctg tacttatggt atattcaata cttttatatc atcaagtaat
     5521 cctaaaaaaa ttattattga tagcaaatgt atacatttta cctcatttat gactacacat
     5581 aagtatgaaa ttaaagatat agaaaagata agaataagag aattttataa taaaaaaatt
     5641 tatctaaaaa ttaatgatgg aaaacttttc aaaggtaggt attggataag aacaaatatg
     5701 tttaataata gtatagcatt atatagctat ttcattgagt tggaagagtg cttatatcca
     5761 gattcactaa aatttagaaa taaaaaattt gagaataaga acatataaaa ataaaaaatt
     5821 gaattgaagc ctagcttaat cttttttgat aagatataag tttgaacttt atactaaatt
     5881 tttagaattt tcaatcaaga caataacata atagctaagc aattagaaca caactaagat
     5941 gtataaggca tgtatatgat gaatgggagg atttaattaa tgttattaga gaaattaaga
     6001 aaagaagtat tacaagcatc tttagacctt ttaaactata atttagtgac attaacagga
     6061 ggaaatgtaa gtgggagaga tgaacaaact ggctatatag caatcacccc aagtggaatg
     6121 gattatagaa atcttacacc aagtgatatt gttatagtag atgtagatgg aaatattata
     6181 gatggaaaat ggaaagcatc agtggattta tcagaccact tgtatatata taagcataga
     6241 gaagacataa atagtataat tcatacacat tctacatact caagttgttt tgctatttta
     6301 aatgaaccta tagaatgtgc aagtacaact cttgcaaatg aagtaggggg ttcagtacca
     6361 gttgcaaaat tttcgccacc aacatctaaa aagatgggaa aatgtgttat tgaagcaatt
     6421 ggtgacaaaa gagcatgtct tttagcaaat catggcgtaa ttgcagttgg tccatctgta
     6481 ggacatgctc ttacagccgc tgtaatgtta gaagattcag caaaagtata ttacttagca
     6541 aaatcaattg gcacaccagt gctactgcct gatgaagaaa ttcaaagagc gagagatgta
     6601 ttttttaatg tctatggtca ggataaataa attagataaa attttaaaag taatatgaaa
     6661 atatagatat tcaatgatat taaggagatt tataatgata taataagttt aatagttaag
     6721 aagtaaaata tgaaatgtaa tagtatataa attcatttaa gactaaaaag aataagataa
     6781 aataaaaagg agtctgtatt tctatgtatt acttattaaa caaaagcaca aatccttact
     6841 ttaatttggc attagaagaa tatctatttt taaatgataa atataatgat gatattataa
     6901 tcatatggag aaatgaagaa tccattttta taggtaaaaa tcaaaatcca tatcaggaag
     6961 tatatcatga tgttattgaa aagggagaaa ttcctatttt aagaagaata tctggaggag
     7021 gaactgttta tcatgactta ggtaatatca atatgtcatt catacaaaaa gataggcaac
     7081 ttcatgagat agattttttg gagcatacta agtttatgca aaagatgctt tcaaccttag
     7141 gactagatgt atctataact gaaagaaaag atttattttt aaatgggaaa aaaatttcag
     7201 gctctgcaca gtctataaaa agaaaaaatt ctctttatca tggaactctt ctttacgatt
     7261 cagatttaaa taaattaact aaatatttaa attcaaataa agcaactgaa tcaaatgcaa
     7321 ctaaatctgt ttcaagtaaa gttacaaata ttaagttttt actcgaaaaa gatataaatt
     7381 actttttaga ttattgtgtt gaatatttaa aatctaatat aaagaatata aaagaacttg
     7441 aattggatga agaagatatt aaaagtgtat acaatatagg agaagataaa tataaaaaga
     7501 tagagtggac atatggtaaa acgccgaaat ttcaagtagt gctaccattt aataatgaaa
     7561 gtaaaataaa tatacatgtt aacagatgga aagtatcaaa attttttatt tcttatgatg
     7621 ataatgtaat taatatggac aagttgttag atttgaattt ctttaaagaa gaaataaaag
     7681 agagtatttt agaaaattat ccacattaca tagatttaat caatttaata ttttaatata
     7741 aaacctctaa tttgataaaa ttgtcttata tcaaactaga ggtttttaat attcttgcat
     7801 attaacaacc attacaaaat tgtaaaatta aaaacaacac tattgaaaaa tcaatatata
     7861 ttgtaataaa tatatgtgtt tttcattagc aaatagacat taactattta tacaactgaa
     7921 aatttaatat ataattaatc agatggttaa aataaaaata ttaaaagttt taataaatga
     7981 tggttaaaaa taaaatacga ggtgatacca tgtatagtgt tataattgtt gcagcgggaa
     8041 gcggaaggag aatgaactta gatataaaca aacagttcat aaaattaaga gaaaaagaaa
     8101 ttattgctca tacaattcaa gttttttatg aaaatataaa tatagatgaa atagtggtat
     8161 gtataaaaaa agaagaagaa gattttttta aagaaaatat aataaataag tataacttta
     8221 aaaatataaa aatagcatat ggcggaaaag aaagacagga ctcaatatat aatggtttaa
     8281 aaaaattaga taaaaattgt gatatagtac ttatccatga tggagcaaga ccatttgtcg
     8341 accatagaat aatcaatgaa tctataaagg tagctaaaga aaaaaaggct gtggttgtag
     8401 gtgttccagt aagtgatact ataaagatag tgtcagatgg aacagtacaa gaaactccag
     8461 aaagaaattt gctgtgggca gcacaaaccc ctcaaacctt tgaatataat ctcataatag
     8521 atgcttatga acaagcatat aagaacaatt attatggtac tgatgattca atgttggtag
     8581 aaaatatagg tcaaagtgtc actatggtaa tgggttctta tgaaaacata aaaataacta
     8641 gtccagaaga tttaaatata gcagaacaaa tattaaatat ggaaaaaaga gatgaagtta
     8701 acagtagaag gagaattata tgagaatagg attaggatat gatgtacaca aattaacaga
     8761 ggatagaaaa cttataatcg gtggtgtaga gataccacac gataaaggtt tgttgggtca
     8821 ttctgatgct gatgtattga tacatgcaat aatggattct attttaggtg ctctagcatt
     8881 aggagatata gggaagcatt tcccagatac agatgaagaa tataagggag cagatagtat
     8941 gaaattatta gaacatgtgt ataatctaat aacaagtaaa ggatataaaa ttggtaatat
     9001 agacagtact ataattgctc aaagccctaa gatggctcca tatattgaga gtatgagaag
     9061 taatatatca aaagttttaa acacagacat tgataatata aacataaagg caacaacaga
     9121 agaaggtctt ggttttacag gagcaaaaca gggaatagct tcacaaagta tttgtttatt
     9181 attgttgaca agtcagaata attaatatat tattagtaat aagtttaatg gatatcaagt
     9241 aataggccta tatagaatat ctataatgta tgaatttaag tgaaattaat ataaatataa
     9301 aattagctat gataaagaaa tagtaaaatg atgaaatctt cacagagaga aaacggtggt
     9361 gagagttttc aagaggaagt ctttgaacct gtcttttagc ttaaaggtat aaaaatcttt
     9421 acggtaggaa ccgttatatt cccaaaagag agctattcat atagctaact agagtggtac
     9481 cgcggaaatt aaacctttcg tctctatact tgtatagaga tgagaggttt tttatatttt
     9541 caggaagtta atattcagga ggaatctatt aatgaaaatg tcaaaaatgt ttatgccgac
     9601 attaaaagaa atcccagcag atgctgagat tacaagccat caattaatgg ttaggtcagg
     9661 catgataaaa aaaatgactt caggtgttta taaccaatta ccaatggggt taagagtgtt
     9721 caaaaaaatt gaacaaataa ttagagaaga attaaataaa aaagattgtc aagaaatact
     9781 ttgtgcagcc ttacttcctt ctgaattatg gaaagagtct ggaaggtgga ctgctatggg
     9841 tgaagaaatg tttagactaa aagacagaac agaaagagag tactgtcttg ggccaacaca
     9901 tgaagaagca ttcactgata taataagaca agaaattact tcatataaac aattgccact
     9961 gaacttgtac caaatacaag taaaatatag agacgagaga agaccaagat ttggtgttat
    10021 gagaactaag acttttacaa tgaaggatgc ctatagtttt gatgtagatg ataaaggttt
    10081 agataagtca tatcaagata tgtttgatgc atatgtaagt atatttaata gatgtggatt
    10141 ggagaatagc cctgtacagg cagattcagg agctatagga ggttctacat cagccgaatt
    10201 tatggttaag tcagaagttg gtgaagatga agttgtattt tgtagtggat gtgattatgc
    10261 agccaatgta gaaagagcag aatcttgtaa tctagcatca caaaaagaag aaatgaaaga
    10321 attagaagag gttcatacac caggagcagc tacaataaaa gagttagaag aatttttaaa
    10381 aacttctcca gataagtttg ctaaaacttt agtatatgag gcagatggaa aaacagttgt
    10441 agtcgttgta agaggagata gagaagttaa tgaaatcaaa gtatctaatg ccataggttc
    10501 tgttatagaa tttgctcttg ctacagatga tgtagtgaga aaagtaacta atgcagaagt
    10561 aggatttgca ggtccaatag gtataaatgc tgactatgta tttatagata aagaaattgt
    10621 tgaacaaaga aacatagttg ttggggctaa taaaactgag taccatataa aaaatgctaa
    10681 ctatggaaga gattttgaag gaatagttgg agactttaga aatgttcaag aaggcgacaa
    10741 gtgtatagta tgtggtaagc cacttgaaat agctagaggt gttgaagtag gacatatatt
    10801 taaactagga actaagtact cagaatctat gaatgcaaac ttcatagaca aagatggaaa
    10861 atcaaagcct atagtaatgg gatgttatgg aataggtgtt gaaagaacag cagcagctat
    10921 aattgaacaa cataatgatg agaaaggtat aatatggcct ttgtcagtag ctccatacca
    10981 tgttgtgata gttccagcaa atatgaaaaa tgaagaacaa atatcaatag ctgaaaatat
    11041 atataatgat ttgcaagcta tgggtgtaga agttttatta gatgacagag atgaaagaat
    11101 aggtgtcaaa ttcaatgatt cagaattaat aggaatacct atgaggatta cagtaggtaa
    11161 aaatattaat gaaggtaaag tagaatttaa acttagacat aaagaagata aagagataat
    11221 tgacatagaa gaaataaatg aaaaagtaaa ggcagagttt atcaggaata atgttaggtt
    11281 aggataataa cttaaataag atattgtata gataatcaaa gttgtacttt atagacacca
    11341 ttttatctat atataaggat ataaaatggt gtttttttat aaaacaaata aatgaggagg
    11401 taattaaaat ggcaaaaaat gaaaaacaat ttgttgaaga aataacaaag atggaagatg
    11461 attttccaca atggtacaca gatgtaataa ctaagactga cttagttgat tatgccccag
    11521 taaaaggttt tatggttgta aagccatatg gatatgcatt atgggaaaag atgcaagaat
    11581 ttatggataa aaaatttaaa gaaactggac ataaaaactg ttattttcca ttattaatac
    11641 cagagagttt attaaacaaa gaagcagaac acgttgaagg ttttgctcca gaagttgcat
    11701 gggtaacaca tggaggaaat aaaaaattag aagaaagatt atgtgtaaga cctacttcag
    11761 agactataat atgtacaatg tatgcgaaat ggttaaaatc atatagagaa ttaccatatc
    11821 tttataatca atggtgctca gttgttagat gggaaaaatc aactagacct ttcttaagaa
    11881 catctgagtt tttatggcaa gaaggtcata cgttacatga aacagcagaa gaagcacaag
    11941 aagaaacaat acaacaatta gaagtatata aagcgctatg tgaagaatta cttgcaatgc
    12001 cagttgtagc tggtcaaaaa agtgaaagtg agaaatttgc aggtggtgaa agaacatata
    12061 ctatagaagc tatgatgcat gatggtaaag cattacaatc aggtacaagt cacttcctag
    12121 gtcaacactt tactaaggct tttgatatta cttttgcaga tagggaagga aatttagcta
    12181 atccatacca tacatcttgg ggagcatcta caagacttat aggtggactt ataatgactc
    12241 atagtgataa cagaggttta gtattaccac caagagtagc tccaattcaa gttgttatag
    12301 ttcctatagc tgctaaaaaa ggaaatgtaa tggagactgt tgataaaata tatgctgatt
    12361 taaaagctaa gggagtagct gtagaagttg atgatagaga taactataca actggatgga
    12421 aatttaatga gtgggaaatg aaaggtgttc cagtaagagt tgaaattgga ccaaaagata
    12481 tagaaaataa tcaagctatg gtatttagaa gagatacttt agaaaaggat tctatgcctt
    12541 tagaaggatt agctgatgct atatgtgatt tatttgatgt tatacacaat gatatgtttg
    12601 aaaaagcaag aaaacataga gaagacaata catctatagt tgaaaatatg gatgagttta
    12661 gaaaagcttt agaagaaaag ccaggattca taaagactat gtggtgtgga gatgcagaat
    12721 gtgaagctaa gattaaggaa gaaacaggag caactataag atgcttacct tttgaacaag
    12781 aaaacttagg tcacaagtgt gtgtactgtg gaaaagaagc agatagtatg gtagtaatgg
    12841 caaaagctta ctaaaataca tttaaataaa aactggggta gaaagtattt aatactttat
    12901 cccagttttt tgttttttat gagtaataaa atgtatttgt aactatgtca taagataagt
    12961 catagatact tatattaaaa agaatataat agagaactat ttttaataat tacatattaa
    13021 tgaaagcaaa atattttgtt atatagattt tcaataaagc aaacaataaa tataaatgaa
    13081 tattaataag gagtgctttt aatttttaga aaataaatga cttaaaagaa agatttatga
    13141 aattaaaact aggattttct gatataataa acatgtgtat ttttaaataa aaaggatatt
    13201 attattagat ttgattaaaa ataaacttgc aaaacaatgt actagtttaa tattatagtt
    13261 atattgaata aaatatcagg aggaagtaaa aatgaatgta agagtaaggt ttgcaccaag
    13321 tccaacagga tttgtacata taggtagttt gagaactgct ttatataact atttatttgc
    13381 aaaaaaaatg ggcggagaat atatattaag agtggaagat acagaccaaa gtagattagt
    13441 agaaggtgct atagagaata tgcttaatgc tatgaaatgg gctggggtca accatagtga
    13501 aggtgttata ttagatgata gtggaaaagt agtacaaaaa ggagaatatg gtccatatat
    13561 tcaatctcaa agattagata tataccaaga atatataaag caacttttag atagtggaaa
    13621 agcttattac tgtttctgta caaaagaaag actggatgaa gtaagagatg ctcaaagagc
    13681 agcaggagaa acagctaaat atgatggtca ttgtaagaat ctttctaaag aagaagtaga
    13741 agcgaatata aaagcaggaa taccatatgt tataagatta agattgccag agaatcatac
    13801 aattaagttt acagacttag ttagaggaga tatggaattt aatactaatg acttagatga
    13861 ccaagtatta atgaaaacag atggatttcc aacttatcat tttgcagtag ttgttgatga
    13921 ctatttgatg aaaattacac atgttataag aggagaagaa tgggtatcat ctactccaaa
    13981 acatgtttat ttatatgaag cttttggatg ggaagcacct gtatttgtgc atttaccaaa
    14041 tatacttaat aaagaaaaga aaaaattaag taaaagacaa ggtgatgtag ctgttgaaga
    14101 ctttaagaaa aaaggatatt taccagaggg attagtaaac tatgtagcat tagttggttg
    14161 gtctcctgaa gataatcaag aattgtttac aatggaggaa ttagaaaaag ccttctctgt
    14221 agaaagagta tctaaaagtg gtggagtatt tgatactgaa aaattaaatt gggttaatca
    14281 gcactatata aaagatggtg atgatgctta tctaacagac ttagcaatac catttttaat
    14341 agaagatgga tttataacag aagaagaagc tactaataaa tatgatttct taaagagtat
    14401 gatatcagta cttaaagaga aattacaata tgtaaaagaa gttactgaac atgcaagtat
    14461 attttttgga gataaaatag aggtagaaac tgaagaggga agagagttct taagattaga
    14521 acatatacct actttaatag atgctttgag agaaaaaata gaaaaaacag aggtattaaa
    14581 tgctgagttt gttcaagcta tgcttaaaga aatacaaaaa gagtacaaaa taaaagggaa
    14641 aaatttattt atgggctcaa gaataatttt gacagggcaa atgcatggtc cagatttacc
    14701 aaaagtaatg gaagttttag gaaaagaaac ttgcttaaat agaatagctt atgtaaaaaa
    14761 taatatatta tagtcataaa agtccgctaa tttagaagcg gacttttttt gtgttagtat
    14821 ttggtgaaaa tatctgctta agaaaatgaa ggtatttaag gtaataaatt taatatataa
    14881 aaagcaaagc aaaaatataa ataaaatatg aataataaac taataaaata ttaagcatag
    14941 aagaagggaa tctattattt tagtttgatt taattattaa gggggtaatt actatgtata
    15001 gtaatattga tgatgttaaa aaggaattaa aggagctatg cttagaatat gttacaattt
    15061 tagaaaaatt aaaagatgaa aaaatgatta cagaagaaac ttttgaaaag tgctcttctc
    15121 aaaaaaaaat atttttagaa gaacaataga ggatggtaat agtattatta ttatgtagtt
    15181 tgaaagatag tacgaaaaat gatagtaata ctattattta ttgcaatttt caactataat
    15241 ataatatata atgttaagaa gataatttat aaaaatacag tgaggtgatg agtgtgaaag
    15301 tttataatac attgactaga acaaaagaag aatttgttcc attagaagaa ggaaaggtaa
    15361 aaatgtatgt gtgtggtcca acagtttata actatataca cataggtaat gcaagaccat
    15421 ttataatatt tgatactcta agaaggtatt tagagtatag aggatatgat gtaacttatg
    15481 tacaaaactt tacagatgtt gatgataaga ttataaatag aagtcatgag gagggaatat
    15541 ctcctgaaga agttgcggct aagtatataa aagaatattt tgtagactgt gatgggctag
    15601 gaataaaaag agctactgtt catcctcagg ttacagataa tatacaacag attatagagt
    15661 ttataaaaga gttggaagat aagggatatg catatgcagt aaatggtgat gtatattttg
    15721 atacaaataa gtttgaaggt tatggaaaac tttcaggtca aaaacaagaa gatttagagg
    15781 caggggctag aattgaggtt aatgaccaaa aaagacatcc tatggacttt gttctttgga
    15841 aggcaaaaaa agaaggtgag ccaggatggg atagcccttg gggagaaggt agaccaggat
    15901 ggcatataga atgttctgtt atgtctaaga gatatttagg tgaaacaatt gatatacatg
    15961 caggtgggca agatttaaca ttcccacatc atgaaaatga aatagctcaa agtgaagcta
    16021 gaagtggaaa aacattctca aaatactgga tgcataatgg atatataaat ataaatgatg
    16081 aaaagatgag taaatcaaaa ggtaatttct ttacagtaag agatatatca aaattatatg
    16141 atttagagat agtaagattt ttcatgttat cagcacatta tagaaatcca gtaaacttta
    16201 gtgatgagat gttaaatcaa gctaaagcag gtcttgaaag actatataat actaaagaaa
    16261 aattagaatt tacgctaagt aatttagtag aatctccttt aacagaaaaa gaagtagagc
    16321 ttgttaaaga attagatgac tttagacaaa aattcataga tgctatggat gatgatgtta
    16381 atacagcaga cgcagtaagt gtaatatttg aattagctaa gttaataaat tctaatgtag
    16441 atgaaaattc ttctttggag tttgctaaaa aatgcttaga tgaatttaat gaacttacag
    16501 gagtattaaa tattgtaaat aagaaaaagg atactgtatt agataaagac attgaagaat
    16561 tgatacaaaa aagaactgat gcaaagaaaa ataaagagtt tcaattagct gatgatatta
    16621 gacaacaatt actagatatg ggaattgtat tagaagacac aagacaaggt gttaagtgga
    16681 aaaggatata aaaatggaaa aaactgaatt agttactatg tcaccattag ttttagcata
    16741 cttaggtgat acagtgtatg agacatatat aagagaatat ttaattagac agaatacaca
    16801 aagaaaagtt aatgatttgc ataaactagc tataaagtat gtaaaagcta aagctcaggc
    16861 aactataatt catgaagttg aaattgagtt aactgaagaa gagagtaaaa tttataaaag
    16921 gggaagaaat caaaagtcaa atacttctcc taagaatgca gatattatag actacaaaca
    16981 tgcaactggg tttgaggcat tagttggata tttgtattta aataatgaaa tagagagact
    17041 tcaatatatt atcaataaag gaatcaaaat tattgaaaga gatatgtaaa tattaaagag
    17101 tgttgtctta ataataagta ataaggcaac acttttttta taactaatgt ataataaata
    17161 tatagaaatt agtaatgaga aaggataaaa agttatgaaa gtaaaattaa tatcacatac
    17221 acctgaacca gaaaaagtaa tagctatggc agctaagcta tgttattcac cagttgggac
    17281 agacgaaata gaaaaagact taacagatga aagtatagag aaatttttaa atatgttatt
    17341 aagtataggt catgggtcaa tattagaaca tgcttcattt acattttcaa tagaaggtat
    17401 ttcaagggca tgttcacatc aaattgttag acatcgtata gctagttttt cacaacaaag
    17461 tcaaagatat gttaaattag aacaatttga gtacataatt cctccagaaa tagaaaaaat
    17521 agaaaaggca aaagagttat ttatagactc catgaaaaaa gaccaagaga attatgataa
    17581 gttagttgaa atattgtttg aaaatcatta taatgaccta ataaaaaacg gtaaaaatga
    17641 gaaaacagct aaaagacaag ctgagaagaa agcaatagaa gatgctagat acgtatttcc
    17701 aaatgcatgt gaaacaaaga tggtatttac tataaatgct agaagtttgt ttaatttctt
    17761 tgaacataga tgctgtgaaa gagcacagtg ggaaataaga aatttagctg tggagatgtt
    17821 aagagaggtt aaaaaggttg ctcctatatt atttaaaaag actggtccaa gttgtgtaaa
    17881 tggaagttgt ccagaaggaa ctatgacttg tggagatata gttcaagtta gagaaaaatt
    17941 taaggccttg taggaggtga ataatttggc gagtatagaa ggaagaaatc cggtaataga
    18001 agcaataaaa agcgacagag aaatagataa aatattaatt gcaaattcag ctaaagaagg
    18061 ttcaattaag aaaataatag gaatggctaa agataagaat ataattattc agtatgttga
    18121 taaacataag ttagatgaag taagtacaag tcattcacat caaggtgtaa tagcttatgc
    18181 aagtgagtat aagtattatg aattagatga gctaatagat ttagcaaaga ataaagatga
    18241 agacccattt ttcattatat tagatgagat aacagaccca cataatttgg gttcaataat
    18301 aaggacagca gatgcagtag gagcacatgg agttatcatt ccaaaaagaa ggtctgtaca
    18361 cataactcct gtagttgcca aagcatctgc tggagcagta gaatatatgc cagtttgcaa
    18421 ggtaaccaat atagttaata caataaaaag actcaaagaa gaagggctgt ggattgctgc
    18481 agctgatatg gatggagaaa ctttttataa acaaaatctt actggtcctc ttggtgtagt
    18541 aattggaagt gaaggttttg gaatatctag attggttaaa caaaactgtg actttatagt
    18601 aaaaatgcca atgataggta atgttacatc tttaaatgca tctgtagcag gtggaatact
    18661 tttatatgag atattcagac aaagactgga taaaagtaaa taggcatgaa aaaaaatata
    18721 aatcattact taataataga tggctacaat attataaatg cgtgggacaa tttgaaagaa
    18781 ctggctaaag aggacttaga agattctcga gaaaagctta ttgatgacat tatagaattt
    18841 tctgagttta tgggttataa gactataata gtttttgatg cttataatgt taaaaattct
    18901 cgagaaaaag ttgaaaaaag aaaacacata acaatagttt atacaagaga acaccaaaca
    18961 gcagatagtt atatagagaa atttataact tctttatcaa agtatgatga tgtaaaagta
    19021 gccactaatg actatgcaga acagcaaatg attttaggaa aaggtgctac tagaatgtct
    19081 gcaagagaat taaagttaga gttagatcgt tccaagaata aaatgaagga aaaaaatatc
    19141 ggtttgagga aaaaaattca gcgaaattgg ttggaggaaa ggttagataa agaaacattg
    19201 tcgaaacttg agaacattcg tagaaagcgt tgaaattact aggttcttta gactataata
    19261 tacctataaa tttgttttat atatggggga gatttacatg ttggtagcaa aagaaaaaag
    19321 ttatgagtta gtggacaatt gtcagcagga tgagtataac attgtattaa gagcaagtga
    19381 aggggataaa atagcactag agtacattat tactaaatac agaaattttg taaaggcaaa
    19441 agcaaagtca tattttttaa ttggagcaga taaagaagat ataatacaag aaggaatgat
    19501 aggcctttat aaggcagtta gagattttga tggaagtaag accaattcat ttaaatgttt
    19561 tgcggagata tgtataacaa gacaaataat aactgctata aaaactgcaa caagacaaaa
    19621 acatatacct ttaaattcat atgtttcttt gaataaacca atatatgatg aagagtcaga
    19681 cagaacactt ttagatatta tagctacaag tatagtaaca gatccagaag agcttataat
    19741 tagtaaagaa gagttaaaaa atatagaatc aaaaatgaac gaattgttaa gtgacttgga
    19801 attagaagta ctagaattat atttaaatgg aaagtcatac cagtttatag cggacaagct
    19861 taaaagagat gtaaagtcaa tagataacgc tttacaaagg gttaagagaa aattagaaaa
    19921 acatcttgaa aacagaaatg attaatagta aaatacttac cataagctta ttgtgagcaa
    19981 aattaattta tatcgaaaga taagggagga aattcaaaat ggctaaagct aaatacgaaa
    20041 gaacaaaacc tcatgttaat atagggacaa taggacacgt agaccacggt aaaactacat
    20101 taacagcagc aataacaaaa acattatatg acagatatca attaggagaa gcagtagatt
    20161 tcgcaaacat agataaagct ccagaagaaa gagaaagagg aatcacaata tcaacagcac
    20221 acgttgagta tgaaacacca aatagacact atgcacacgt tgactgccca ggacatgctg
    20281 actacgttaa gaacatgata acaggagcag cacaaatgga cggagcaata ttagtttgtt
    20341 cagcaacaga tggaccaatg ccacaaacaa gagagcatat actattatca agacaagttg
    20401 gagtaccata tatagtagta ttcttaaaca aatgtgacat ggtagatgat gaagagttat
    20461 tagagttagt agagatggaa gtaagagatt tattaacaga atatgatttc ccaggagatg
    20521 acactccaat agtaagaggt tcagcattaa t
//
LOCUS       DAYATN010000061        19875 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.061, whole genome shotgun sequence.
ACCESSION   DAYATN010000061 DAYATN010000000
VERSION     DAYATN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19875
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.061"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            131..1324
                     /locus_tag="ACGI8A_002118"
     CDS             131..1324
                     /locus_tag="ACGI8A_002118"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFH0180115.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1530..2648
                     /locus_tag="ACGI8A_002119"
     CDS             1530..2648
                     /locus_tag="ACGI8A_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HFH0180116.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2779..3024
                     /locus_tag="ACGI8A_002120"
     CDS             2779..3024
                     /locus_tag="ACGI8A_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFH0180117.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3011..3757
                     /locus_tag="ACGI8A_002121"
     CDS             3011..3757
                     /locus_tag="ACGI8A_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0180118.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4042..4917
                     /gene="hslO"
                     /locus_tag="ACGI8A_002122"
     CDS             4042..4917
                     /gene="hslO"
                     /locus_tag="ACGI8A_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFH0180119.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5308..6108
                     /locus_tag="ACGI8A_002123"
     CDS             5308..6108
                     /locus_tag="ACGI8A_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFH0180120.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6636..8876
                     /locus_tag="ACGI8A_002124"
     CDS             6636..8876
                     /locus_tag="ACGI8A_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0180121.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9290..10825
                     /locus_tag="ACGI8A_002125"
     CDS             9290..10825
                     /locus_tag="ACGI8A_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0180122.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            10841..11602
                     /locus_tag="ACGI8A_002126"
     CDS             10841..11602
                     /locus_tag="ACGI8A_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0180123.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            11940..12809
                     /locus_tag="ACGI8A_002127"
     CDS             11940..12809
                     /locus_tag="ACGI8A_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0180124.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            13138..13389
                     /locus_tag="ACGI8A_002128"
     CDS             13138..13389
                     /locus_tag="ACGI8A_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HFH0180125.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            13501..14280
                     /locus_tag="ACGI8A_002129"
     CDS             13501..14280
                     /locus_tag="ACGI8A_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0180126.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFINYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(14415..15065)
                     /locus_tag="ACGI8A_002130"
     CDS             complement(14415..15065)
                     /locus_tag="ACGI8A_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180127.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(15062..15565)
                     /locus_tag="ACGI8A_002131"
     CDS             complement(15062..15565)
                     /locus_tag="ACGI8A_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0180128.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(15614..16129)
                     /locus_tag="ACGI8A_002132"
     CDS             complement(15614..16129)
                     /locus_tag="ACGI8A_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0180129.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            16409..16936
                     /locus_tag="ACGI8A_002133"
     CDS             16409..16936
                     /locus_tag="ACGI8A_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFH0180130.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            16920..18323
                     /locus_tag="ACGI8A_002134"
     CDS             16920..18323
                     /locus_tag="ACGI8A_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0180131.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(18706..19017)
                     /locus_tag="ACGI8A_002135"
     CDS             complement(18706..19017)
                     /locus_tag="ACGI8A_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180132.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(19073..19666)
                     /locus_tag="ACGI8A_002136"
     CDS             complement(19073..19666)
                     /locus_tag="ACGI8A_002136"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0180133.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT         7572 a         2156 c         3523 g         6624 t
ORIGIN      
        1 taccaaaatg gaaataaaaa ataaatttta tgagaattta ataactaata attaatatat
       61 ttaggttata ataaatatgt gcgtaaaatc taacaataag caataatacc gtaaatttag
      121 gaggaataat atggatactt taaaggaact ttttaaaata ggaagtggac cttcaagttc
      181 tcatactatg ggaccacaaa gagcagcaga gagatttaag aatgaaaacc cagatgcaga
      241 aagttttaga gcaatattat atggtagttt ggcagctaca gggaaaggtc acttaactga
      301 ctatataatt gaaaaaacta tagcacctaa aaaagtcgaa attgtttggg aagaagatat
      361 tataaaagat tttcacccaa atgggatgaa gtttgaagct ttagataaag ataaaaatgt
      421 aacagctgaa tggactgtat attctgtagg tggagggact atagcagaag aaggtcaaag
      481 aaacagtaaa agcaatagta tatatcactt agatactatg gatgagatag taaaatggtg
      541 caaagaaaat aataagactt tggtagattt tgtgttggaa tgtgaaccaa aagacataaa
      601 agattatata aaaactataa aagatgctat gagaaaatct attgatgatg gactaagtac
      661 agatgaaata ataccaggca agttattatt aaaaagaaga gcaagtacat tttataatgc
      721 atataaaaaa gataagagct tctcaacact tgtatatgca tatgcactag cagcttcaga
      781 gcaaaacgct tctggtaata taatagttac agcaccaact tgtggttctg ctggagtaat
      841 accaggaata ttctttgcaa tgcaggattt ctataattat gatgacgaaa aaataattga
      901 agcactactt gtagctggaa taataggtaa tataataaaa actaacgctt caatatctgg
      961 agcagaggta gggtgtcaag gtgaagttgg agccgcttgt tctatggcag cagcagcagt
     1021 agcatactta aaaggtggaa ctatagacca tattgaatat gcggcagaaa tagcacttga
     1081 acatcattta ggaatgactt gtgaccctgt atatggatat gttcaaattc catgtataga
     1141 aagaaatgca atggcagctc aaagagctta tgatgcagct aattatgcat tattaactga
     1201 tggctcacat tctgtctcct tagaccaagt tgttgaaaca atgaaagaaa ctggtataga
     1261 tatgatggat aagtacaaag aaacagctaa gggtggactt gctaaacatt tcttctcttg
     1321 ctaaaaataa aattttccaa taataattcc tctgttttga tttatgaaac aggggaatta
     1381 ttattaatgg tgaattattt aataatgcaa aatattttta tattcaattt agctttttat
     1441 ttttaattag tataacatgc tatataatat tttaattata aagaaatata gaaatatttt
     1501 ttaataaatt agaaattgga ggagattaca tgagagaggc atcatattta caagaacaac
     1561 tttataaaca taggaagtca ttaaatccta ttgcagagat tgggagaaaa gagtataaga
     1621 cttcaaaata tataagagat tatcttgata aattaggaat tgagtatgaa gtttatttag
     1681 aaacaggtat agtaggtgta atagaaggta aaagacctaa aaaagatatt gcttttagag
     1741 ctgacataga tggtcttcat agtgaagaag gggtaaaaca tttatgtggt catgatggac
     1801 atgctactat attacttgga ttaattgagt atttaaatga taataaagaa gacttaaatg
     1861 ataatatagt atttatattc caaccagcag aagaaggtcc tgggggagca aaagctttaa
     1921 tagatgaagg agtgcttaga aaatacaata ttgatgaaat atatgggctt catatatatc
     1981 cagagttacc agaaggttat gtaggtacaa gagagggcta ttttatggct caaataggag
     2041 atattgacat agaaatagtg tctaaaagtg gacatggggc tatgccacaa aatggtattg
     2101 atggtatagt gatagcttca aactttgtaa gctcacttca aactatagtg tctagaaata
     2161 taagcccaat agacaatgca gtgttgacta ttggtagaat tgaaggggga gctagaagaa
     2221 atataatagc tgaaaatata aagatggaag gtactatgag atgtttcagc ccagaagtgt
     2281 atgagaaaat gaagcttaga gttagagaac ttgctagagg gtttgaattg gcttataact
     2341 gtaaggttaa tataaatatt atagatgatt atattgctgt taaaaatgac aaggatttgt
     2401 atcaagagtt tgtagaggct attggaagtg atactgttgt agagttagag ccacttatga
     2461 tatcagaaga tttttcttat tatcaaaaag aagttccagg attatttttt atgttgggtt
     2521 caagaaacga agaaaaagga tttgtaaatg gtcttcataa tataaacttt aattttgatg
     2581 aaaagatatg tgtaaatgca ttgaatgttt attcaaaatt attaaaatac aaagaagcaa
     2641 tagattagga agataagtta taaagattaa aaagcataaa tacctaaatt taaaaaaagt
     2701 gtattaataa atacccctta catatttgta taaatttatt ttaattgaca aaaatacaaa
     2761 taagaagggg tgattttttt gaaagaaaaa gtacaaagag aaaagaagat aactaatggc
     2821 aagaatacta aaatattgac aaatgatgat ataatgaaat atgagatagc ctcagaacta
     2881 ggtcttatgg ataaagttgg tgaacgtgga tgggcaggac ttacagctaa ggaagcagga
     2941 agaattggag gtatgcttac atcacgcaaa aagaggctta aaaagattgt ggaggagcag
     3001 aataaaaagg atgagtcaat atagtgattt tgcgtttgta tatgatgaac ttatgaatga
     3061 agttgattat aatggttggg ttaaatatat agaagatata atagatagtg aaggtgtaaa
     3121 agtaaaaaat atattggaat tggcatgtgg cacgggtaat cttacaattc cattgactaa
     3181 aaaaaattac gatatagctg gtatagatat atctgatgag atgcttagtg tagcaagaga
     3241 aaaggcagaa aaagaaggag tagaattagt tttacttcaa caagatatat cagagcttga
     3301 ttttgaaata tctgatttgg actgtgtact ttgtgcttgt gatggattta attatataac
     3361 ttatgatgat gatttggaaa atgtattttc aaagacatat gaattattaa agaaagatgg
     3421 aatattcata tttgatataa gttcttttta taaattagcc aacatattgg gaaataacat
     3481 gtatggagaa aatagagaag atatagcgta tatgtggcaa aattactttg atgaagaaga
     3541 gaatctaatt gaaatggaac ttacattctt tattaaagat gaatgtggaa agtttgaaag
     3601 atttgaagag atacaccaac aaagagcata tactgaagat gagatagttg atatgcttga
     3661 aaaatctgga tttaaggata taaaatatta tggggatttt acatttgaca aacctagtga
     3721 agaaagtcaa agaatcttct ttgtatgtaa aaaataagat attcttgact atataagata
     3781 attattaaaa ataaccatga tataaaaata tataaaatgc agttttatag attaaaagta
     3841 aagtgatgat attttatgat ttttattttc taataattat aaagttttat agataaaaat
     3901 taaaatataa taactattgc ttagtaaaat taaaataaaa tgggttttgc ccaaggttaa
     3961 gatagataag tatttataga ttaaaattaa ggtagtaata taataaaaat aagacaatta
     4021 acgggacatg gaggaatatt aatgagagat tatgtactaa gagcgacatc aggaaatggt
     4081 caagttagag catttgtcgc aactactaga aatacagtag aagaagctag aagattacat
     4141 gaaactacaa aagtagcaac agcagcactt ggaagaacat taacagccac atctataatg
     4201 ggattaatga tgaaaaatga tagtgataaa ttgactgtaa taataaaagg tggaggtcca
     4261 attggaacta ttatcgccac atcagactct aagggaatgg taaaaggata tgtaggaaat
     4321 ccacaagtag aggttgaaga ctatccaaat ggaaagttaa atgtagcagc agcagtaggt
     4381 acagaaggtg ttgtaaaagt tataaaagac ttaggtctta gagaaccata taatgggact
     4441 tatccattgg taagtggaga aattgcagaa gactttactt attattttgc tgtatcagag
     4501 cagactccat cagtagtagc tttaggtgtt cttacaaaag aagatgaagt agagtttgct
     4561 ggaggattta tagttcaatt aatgccagat gcagaagaag agactattgc taagttagag
     4621 gaaaatgtag ctaaattacc ttctataact aacatgttaa aagaaggaaa atcaccagag
     4681 gatatattaa atattgtttt ggatggtttg gaacctaaaa tacttgatac ctgtgaagtt
     4741 gggtttatgt gtgaatgttc aaaagaaaga gtaaaaactg ctttggttgc aattggtaaa
     4801 aaatcattgg ctcaaataat agaagaagat aaaaaagcag aagttggttg ccagttctgt
     4861 aataagaagt atatgtatag tgaagaagag ttgcttgaaa ttttaaagga aatgtagact
     4921 ataaaaatag ttgtattgat ataagtaatg ttatagtaat atagtaaagt cgtattgata
     4981 tagtaattaa agtataattt attaataata gacatattta tattaaaaaa taagtttaat
     5041 aaatttttaa gcttaataaa ttttaagaat taagaagctt tctggctgta atgtttgcaa
     5101 tgttgcagtt agaaggcttt tttattttat tatagttcgt gtcaaagatt gtatgaaaca
     5161 ataaaatatt gtaaaataat ttgtatattt agacctgatt gacacaaagg tttataagat
     5221 atttgagttg attatacgaa attctatgaa gtagtaaccc tgtgacttta atcatgggag
     5281 gttcagaaga aggagaaaat tttaaatatg gaaagtaaaa cgaagtacat aggaataagt
     5341 atattaatat tattgatgat atttttaatt gcaaagaatt ttaacggaaa acttgaaata
     5401 cctattcctg agagtaatag tagaaaattt aaatcagcag cagtgtttta tcctcaacat
     5461 caagatgatg aggttttatg gggaggaagt gccattgtag atgctataaa acagtgtggt
     5521 gttgataatg tatatgtggt attagtatca gatggttctg gagttaacgt tttcaaagct
     5581 aacacaaaat ttagaaattt aactaggaag caaaaggaag aacttagaaa taacgagttt
     5641 aaatcagctt tgagagagct tggagtgaaa cctcaaaatg tgattatact tgcagatata
     5701 gataaaaagg aaggcacaca ttatgaatta atggaaaaga ctatattgga ttttgaacat
     5761 aagtttaaag gcaatgtgac ccatatagcc catcactata agtatgatga ccatataatg
     5821 catagaaaga atggggaagt tttaaagaaa cttaaacgtg aaggtaagat aaaagatgac
     5881 ttgtattttg ttaagccaaa atatgtaaag tatataccaa gaaatgaaag agctatctat
     5941 aaagcaatgg attttgatga ctacaataag gttaagagtg cttgttatga atataaggtt
     6001 gtaaattcaa agcgtaaaat gtatggtata ggatatattt cggcacatag ttattttgaa
     6061 catcttctaa atgaccctga acttacgtct gtattaagtg aagattaatt tcttataagt
     6121 agtaagttat gtttgataag ttgattggaa ttttctatag ttttgaaggt attggaaatt
     6181 tagttttatt taaagattta aagatagagg tttttagtgt ttaaaatttt aaagttaagt
     6241 tgaataattg tatagaatta tttgtgaaag aagaaacagt gtaaaacata gtatcttatt
     6301 tatggtttta aaatcatagg caagatactt tttttatgta actaatacca ttttgagatt
     6361 attaacttaa gaattttata taatttaaca ctcatatata atgatgatat ttaggtagaa
     6421 tagatgtaat tacgcttaaa taaaataact gtatttttta taaaaatcca aaccctacac
     6481 tatactacca gataaaatct acatgctata atttgacctg tgtttataaa aaaaggagta
     6541 atctaataaa atagtatttc acttatgtat aactaacaac tattattacg attttctcta
     6601 aataatttaa agaaaaataa tgcaggaggt aaaatatgaa caatgaattt ataattttaa
     6661 aaggttgcaa agagaacaat ttgaacaata tttccttaaa aataccaaaa agaaaaataa
     6721 ccatattcac aggggtgtcg ggttctggaa agtcatctat tgtatttgag acaattgcaa
     6781 aagaatcaca acgccaacta aatgaaaggt ttagtacatt tgtaagaagt tttttaccta
     6841 agtatggaga ggttaaagct gattgtatag aaaatttgtc aacaccaatt atcatagacc
     6901 aaagtagact tggtggaaat tcaaggtcaa ctctaggtac tattacagat gtaaattcct
     6961 ttttaagagc tttatattca aggtttggta gtgagtatat aggtaatgcc aatatgtttt
     7021 cttttaataa tattgatggt atgtgcccac actgtcatgg gcttggaaaa aagctagttc
     7081 ctaatatgaa ccaaattctt gatatgaata aatcattaaa tgaaggtgct atattgcttt
     7141 cagggtttgg agtgggttct tggcattgga atatattcgc tgaatcagga ttttttgata
     7201 ttaataagag gatatgtgat tattcagaag tagaattgga aaagtttttg catggtaaag
     7261 cagaaaagat taagatagaa aatgctggtc aaactaacat gacatatgag ggattaatgg
     7321 ttaaatttaa tcgcctatac ttaagtagag agggagaaat atctgaagct actaagaaaa
     7381 aattgagtaa acttcttata gaagatagat gtcctatatg tgatggaaga agacttaatg
     7441 aaagagtata taggtcttta attaatggtt ataatatagc agatttaact agtatgcaaa
     7501 ttgatgaatt ggctgaagtt attaaaagta tagatgagcc agaagcacaa cctttaatta
     7561 aaggaataat agaaaagtta aacagtatca tagatatagg tcttggatat ttaacactag
     7621 atagagagac atcttctcta tcaggagggg aatctcagcg tataaagatg gttaagtatt
     7681 taaatagtaa tcttgttgat ttaatgtata tttttgatga accaagtgta ggacttcatc
     7741 caaaagatgt gtataaatta aataatcttt taaaaaaatt gcgtgataaa gggaatacaa
     7801 ttatagttgt tgaacatgac ccagatgtta ttaagattgc agaccatatt gtagatgtag
     7861 gaccaaaggc aggtaagtat ggtggagaag ttgtgtatga aggcagttat gaaaatctac
     7921 ttacaagtgg tactttgaca gggaatgctt taagtaaatt tcttcctatt aaagaaagtg
     7981 taagagaaca tagtggatat ttagaagtta aaaattgtaa taaaaacaat ttaaaaaatg
     8041 tatctattaa gatacctaaa ggtgtattga ctttagttac tggagtagca ggagcaggta
     8101 aaagtacatt gattaaggat gaatttttaa agcaaaatcc tagtgctgta cttatagacc
     8161 aaagccctgt ttcagcaaat tcaaggtcga gtttggcaac atatagtgga attatgaaca
     8221 acataagaag catattttca aaaacaaatg gcgtaaatgc ttctttattc agttcaaatt
     8281 cagaaggagc ttgtgataac tgtaagggtt ctggaatagt agaaatgaat cttgctttta
     8341 tggagagtat aaagagtacc tgcaatattt gtgaaggtaa aaaatttaaa aaagaagttt
     8401 tagaatatag gtttcaaggg aagaatataa ttgaagttct tgaaatgtct gttttagagg
     8461 caattgagtt ttttaattta aaacagatta aaacgaaatt acaatgtatt gaaaaaatgg
     8521 gtattggata ccttacttta ggacaaactc ttgatacttt gtctggagga gagtgtcaaa
     8581 gattaaagct tgctagtgaa ttaaataagg aaagttcagt gtatatatta gatgaaccaa
     8641 caacaggtct tcatatggct gatattgaga attttataag tataattgaa gagattgtag
     8701 ataatggaaa tacggtcata ataattgaac acaatataga tgtaattaaa agagctgatt
     8761 ggattattga gctgggacct gaaggaggaa caaaaggcgg gagagtaata tttgaaggta
     8821 ttccaaaaca attatgtaac aataaaatat cattaacagc aaaatatata gtataataaa
     8881 taaaatataa taaaattata atgatatttt aataaatata atgggtatat aaaatgataa
     8941 ttaaacttaa cagtaataaa taatgtgaga aatattcaaa tttaattaaa taaagtgtca
     9001 gaaatttgtt taaattttag tattttaacg gttctgacct tttttcttat ttttatggag
     9061 aatatcgagg atggagtctc taatagttac ttttttgtgt ctacttgcaa gtttgattaa
     9121 aaaatggcta tacacacttg tgacttcagt catgggaggt tcagattata atcttaatat
     9181 ataatttgtg aaataatttg atgacgattt gctgatgtat aaacaataaa gattaactac
     9241 attaaaattt aaatcttgtt aagtaaaact tataaaagga gagagatgga tgataaatga
     9301 tattctaacc ttattatctg aaagaagtga tttttttata aagctatcca tagaacattt
     9361 gatgatttct attatttcta taataattgc gattgttttg ggtggtataa ttggtattat
     9421 tgtaagtgaa tttcataatt cttctaaacc tatattggga actatcaatt ttctatacac
     9481 cattccatct atttctatgc taggatttct gataccattt tccggaattg gaaatactac
     9541 tgctgttatt gcacttacca tttatgcatt attgccaatg gttagaaata catatactgg
     9601 aattactaat gtggataaaa atattcttga agcagctagg ggaatgggaa gtacagattt
     9661 tcagatattg tataaaataa aacttccgtt agccctacct gttattatgt ctggtattag
     9721 aaatatggtg attatgacaa ttgcattagc tggtatcgct tcatttatag gtgctggagg
     9781 cctaggtgta gccatatata gaggaatcac gacaaataac acaataatga caattactgg
     9841 aagtatactt attgctctat tagctttaat atttgatttt attttagggc tggtagaaaa
     9901 attttttcaa cgaaaaagaa aaaagaaaaa taggaaaaaa gtttttgcca ttctaggaag
     9961 ccttattctt atagtaatta tctttacgtc ttatttattt aatccttcaa agactcaaac
    10021 tattcatatt gctactaaac ctatgactga acaatatatt cttggtgaga tgttggatat
    10081 tttaattgaa aaaaacacag gtctaaacgt ggaaattaca caaggtgtag gagggggaac
    10141 atctaatatc cagccagcta tggagaatgg agaatttgac atttatccag agtatacagg
    10201 tacaggctgg aatatggtac ttaagaaaaa tgaattatat actgaaagta tgtttagtaa
    10261 actacagtcg gaatacaaaa ctaaacttaa aatgcaatgg actggtatgt atggtttttc
    10321 taatagctat gcattagcag ttagaaaaga agttgcagat aaatacaatt taaaaaatat
    10381 aacggactta aaagatgtat cttctaaatt gacatttgga gctgaatatg acttttatga
    10441 gagagaggat ggatatactc cattgtgtaa tacatatgga ctgaacttta aagatacagt
    10501 tgatttagat attggattaa aataccaagc tatcaatgaa ggtaagattg acgtaatgcc
    10561 tatatttaca actgatggtc agtatagtgt ttcagatgtt gtagttctaa aagatgataa
    10621 aaatttctac ccatcttatc aatgtggaaa tgttgttcgt ttggaagtat tagaggaaaa
    10681 tccagaacta tcaaaggtat ttaagatggt tggagatatt ttgactgata aagaaatggc
    10741 taaaatgaat tatgacgtag aaaacaacaa tttagaacct agagaagttg ctcgtgagtt
    10801 tctgaataag aaaggtcttt tataaattgg aggaaatata atgacaccta ttatacaatt
    10861 taaaaatatt aaaaaacaat ataatgataa aactataata gataatctta atttagatat
    10921 agaaaaagga gagtttttga ctgtaattgg ttcgtctggt tctggtaaga caacactctt
    10981 aaaaatgatt aatggtctga ttttgccaga tggaggaaat attttgataa accaaacaga
    11041 tatcaaaaac gaggacttaa ttaaattaag aagaagaata ggatattgtg tacagggaag
    11101 tgttcttttt cctcatatga ctgttgaaga aaatatttca tatgtaccaa atctcctaag
    11161 taaaaagaat aaattagaag taaaaagtgc tgtaaacaag tggatggaaa tagtgggatt
    11221 gccaaatgat atgaaagtgc gttacccatc agaattgtca ggtggtcagc aacaacgtgt
    11281 agggattgca agagctttag cttcttctcc agaaatttta ttaatggatg aaccttttgg
    11341 tgcagttgat gagattacac gaaagcaatt acaaaaagag ataaaagaaa ttcataaaaa
    11401 aacaggagtt acgattatct ttattacgca tgatatttat gaagctttga ttttgggaac
    11461 aaagactttg gtattaaatc atggagttgt tcaacagtat gatactcctg agaatatttt
    11521 aaatactcct gctaaccaat ttgtagacca gttattgaat ataagaaaat ctataataga
    11581 tgatgataat ttaaaagaat aaatttaaat ttaaaatcaa attgttgtga taaaaactat
    11641 tgatttagag ctaacttcaa gttgtatact tgaagtggaa aagttagatt aaggtataga
    11701 agggcattta gcagttggct gtagacgagg taactgctga aggtgctttc aataaatggc
    11761 tagaacctgt atctgttgaa gattataata aattgaaata agaagctcaa tatacagagt
    11821 agataggcac ttcaaataga agtgtctttt gtgtgccata aaataaatca aaatattagc
    11881 aaagaatgtg aaaaacaaaa taaaataata tgctatcttt aaaataggag ggatgctata
    11941 tggataacaa tttgattacc attgaaaaaa ctttagatta tatagaaaaa catcttttag
    12001 atgagatgtt agatttaaat gtaatttcta aagagatagg ctattcaaaa taccatttac
    12061 atagaatgtt tacatcagtt gtcggattta gtgtacataa ttatatacaa agacgtagac
    12121 ttactgaagc agcgagaatg ctaattttta cagaaaaacc aattattgag attgctttaa
    12181 tctcggggta tgatacacaa cgctcttttt caagagggtt taaaaaaatg tttaaacgta
    12241 gtcctaattt ttttagaaga catcaagatt ttttaccact acaattaaaa tttgatattc
    12301 ataatagaaa aaaattacgt ggagatatgg ttttagatgt taaaaaagtt aattgtgcag
    12361 atataaatat tgttggctat tctggtaaag taagaaaagg atttcatatt attggaaaat
    12421 gctggaggga tttgcataaa aataaagaga tgatagaaaa taaggtagat ttaggttttt
    12481 taattggagt tagtgattat agcaatttta aagagattga gaatactcct acatttacat
    12541 acattgcagg tgctcaagta gctacttttg gcaaattacc taatggaatg gaaaaattcg
    12601 tattaccgaa aagtaattat attgttttta cttttattgg gaaaaatgag gatagtatgc
    12661 aaaatgttgt agaatatatc tatcaagaat ggttccctaa ttcaagttgc cagtttaatg
    12721 aaaataattt atatgacttt gtaaaatatg gagaaaaaac tgatgagaat ggtttgagtg
    12781 aaattcagtt ttgggttcca atcatttaaa agaaggtatt atgtataaag tagatggatt
    12841 tatataggca aagttttaca gcagtatgta tttataaata atttatagtt tgtaggaaag
    12901 aaataatttt actataagat tatgataaaa tacacaatac tatgacaaaa tagtgttaaa
    12961 taatagattt aattttgcat aggaaaagtt ctataatggt gtgtattcat aaataattta
    13021 taatctgtag gaaagaaata gttttactat aagattatga taaaatacac aatactatga
    13081 taaaatagta ctaaataata gatttaattt tgcaaaggat aaggtgtttt aataaaaatg
    13141 aatgtagaaa acgaattact taaaaaccta gatagattgc atacaacaga gttaggcgtt
    13201 gtaagaatta agaaaaatct ttctttggag acaaatgatg tagtgaactg gtgtaaaact
    13261 aaaatagaat ctccaaatgc cattattaat agaaagggaa aaaactggta tataagtgta
    13321 tatgattgta ttataacagt aaacgctcac agttatacaa ttattacagc tcataaagaa
    13381 aagaaatgaa ttgcaaaata caaaggtcaa aagcattttc cactgtttgt agtacctatg
    13441 aagaagtata taaaataata ttacttttga ataataattt aaattttgga ggttgaaaaa
    13501 atgaatgata ctgtaaaaga aaaattggca gagtcactaa cttctgatac tactgatatt
    13561 ttaccttatt tgccttattt attacaagat ttttgggaat taggaagttc accagaacaa
    13621 atgttacaat tatttagaaa gcatatacct tttaatgagg atacaaaagt tttggattta
    13681 gcttgtggaa aaggtgctgt atctgtgaag cttgcaaaag agttgaatat acatatcaaa
    13741 ggtattgatt tgatacctga atttattaat tatgctagaa gaaaggctat tgaatataag
    13801 gttgatgctt tgtgtgattt tgaaatgaat gatgctaata gttctgtaaa aattgagcat
    13861 gattatgatt gtgtgatttt tggagctgta ggggatattt taggtaatta taaagaaact
    13921 ataagtaagc tattacctac aataaaaagt aaggggttca ttttaattga tgatgcatat
    13981 gtagtagata aatctacaaa tgaacagctt aattttgaga gggtgtatcc cacttacaat
    14041 gagtggtgta tattgttcaa agaattaggt ctgaagctaa ttgaatgtat accatgttta
    14101 gaacttgaaa atgatgttga agaggaaatg aattggatta taactcgtgc aaatgagcta
    14161 attaaaaaat atccttctca aaaagatatg tttgaacaat atgttattag ccaaaaaaat
    14221 gaatatactg atttacagca ggaccttgtt ggagcaactt ggattttaca aaaagaataa
    14281 gataaatatt agactagata atcaatgtta tagtgaatta tcccatgtct agtgaatata
    14341 aataacataa aaaaccgtca agatgaatag tttattcgtg cttgacggtt tttagtattt
    14401 gttgaagtaa aattttattt ctttagtata tcacttttag tcaatccatt tgatttagct
    14461 tgagtattcc attgatgatt ccccataagt ttatatagga atctagggaa gttaggtgaa
    14521 ataaaaatag tacttttgtt ggaatcatct atatgtatat tatcactaag tatatttttt
    14581 gaaagttctt ttaatgcaat tcctaaatct tttttaggtc ctttacccaa aggtacggat
    14641 tctagtgaac caaacatctc cccagctcca attccaatac cttgaaccca tgtaaagttg
    14701 atttttttac accaatgctc tatcatttga attgccaaat gattatgttc accttcattg
    14761 aatccacagt taactattgc atacactgga ataggttttt tattttgtgt ttttaaaaat
    14821 ttttcacatt tctctaaaaa atgaagtaaa tgagatggaa taccatctac ataaagagga
    14881 gatgcgatta ttaatctatc acaattaagt atggcattaa gagcgtcagg atacttagat
    14941 gtgctatcta ctttaaatgt aaaaacttta ttatcatttt gaataagttg ttttaattca
    15001 gataaaaaat actgtgaatt atttttattt acttttggac taccatttat cattactata
    15061 ttcatatgga ttcacctctt atatcttgta tattttcata gaaacaaaca gagtgttctt
    15121 ttatattgaa atttatagca tttccattta ctaatttcgt agcagtttct ttttcagctt
    15181 ctgtaataca atctccatag aatatagctt ttaaatttat ctgtttatca tatctgcttt
    15241 tatgacgcat catattatct tttattacaa agtatggaag aatataatta atgcttttat
    15301 caagtacgtt tttgacaaaa gggctaaacc ctccgtaaca gcatctgctt attataataa
    15361 ttccatcaga gtcatttata agtttaccca tgttgctgta ataatctttt acaacacatt
    15421 cagaaggagt tttaatccag caaccaaaac aaccaataca tttgtggatt tcttttgtgt
    15481 ttggaagtat ttttatattg tcatcaagtt tgggtaataa cttttctaat tctttgtcag
    15541 ttaaatcatg tattaaaagc ctcataaagt ccatctcctt ttattatgta tgtttacttc
    15601 taaaaattat atcttataga tttttcagta aatctttaca ccaattaata acaaaattat
    15661 aatagtcaac tccataaatc attgttgcat acatatatgt atattggtca acgcaatgat
    15721 tttcttctgc atcatttttt tgtttactta atttatttag aagtaattct acttcagtta
    15781 ttaacatttt taagtttgtt tcaattattt ctttgggtag atacttgtaa aagaatatat
    15841 taacaaggtg gtcttgcttt gttttagaaa aatttatagg tttctttaac cattcaagaa
    15901 atacttcttt accagtatta gttatggaat ataatttttt taatttgcta ccatctataa
    15961 cttcgtgata gtgtatatat ccttttgttt ctaatctttt aagtgcagga tagatacttc
    16021 caaagctggc gtcaaaaaag tacgaggtac tttctgacat tatttgtttt aagtcgtatc
    16081 cacttaattc tttttccatt aaaaatccta aaataatgta ttctaacata ttacctcctg
    16141 gtatcaatta gatatatatc gattcgatat atttattatt acagataata aatcttacgt
    16201 caagatgctt tttaagataa gctaaaaaga attaggagtt attgataaat atgcaaaaaa
    16261 attaatataa aaggtaatta aatatcaatg aagatttaaa aatagttaaa tcaaagtaag
    16321 agtctaatat ataaaattta tagtaacttt atggtttaaa tataaacttg cgtgctattg
    16381 acaaagtata aaattagcag taaaataatt gcatgagcaa atagattatt gcgaacgcaa
    16441 aaaaataagg acaatagata ttaaaggaga gaatatgaga gagctattaa aatgggtttc
    16501 cattataaat agatacaata caaattatat tgacaggaaa ttggcggata ttggaatcaa
    16561 taatagccaa tatttttatg tattacacat atgtaataat ccaggtatta cacaggacac
    16621 tatatttgag aaaattcttg tgaatcctag taatataaca agagctttgg caaatttaga
    16681 aaaagaaggc tttataacaa gagaaccagg tgtaaaggat aaaagaacat ggcatttata
    16741 tcctacagaa aaatctttag gatgttatga tgaaatacta aaaataacaa atgggtatgt
    16801 tgaggagttg ttgagttcat ttgagaaaga agagcaagag ttgtttatgt caatgttaaa
    16861 gaaagttgca tttttggcta ttgatatgaa tgaagaagca aaattggagg cgaaggaaaa
    16921 tggaagaaaa atataaggag ccattggaat atgaaaaggt ttcaaagtta ttaactaagt
    16981 acgcaattcc tagtattata gcaatgttag taagtgcttt atataacatt gttgaccaaa
    17041 tatttattgg gcaaggtgtt ggtgtattgg gtaatgctgc tacaaatgtc acttttccac
    17101 taacaacaat atgtactgca attgcattat tgttaggaat tggaggagcg tctaagtgtt
    17161 cattggaatt aggtgctaaa aattcagaaa aagctacaaa agcagcagga aattctatat
    17221 gcttgatggc tgtttttgga ataagtttat ttatagtagt atcaatattt ttaacaccac
    17281 tattaaaatt ctttggctct acatcagaaa tactacctta tgcacacaca tatacaaaaa
    17341 ttacatcaat tggattacca tttcttataa tgtccactgg aatgagtaag ttgatacttg
    17401 cagatgggag accaaaagct tctatgtttt gtatgttaat aggagcagtt attaatacaa
    17461 ttttgaatcc attattcatt tttgttttta atatgggaat tgctgggtct gctttagcaa
    17521 ctgttattgg gcaacttata tcttttggaa taagtgttca ttatataaag aattttcaac
    17581 atattaaatt acataagaaa agttttaaat tagatagtat aatttataaa catatatttg
    17641 cgctaggttc aagctcatgt tttaatcaat tagcaatgac tgtaacacag atagttatga
    17701 ataatacatt ggcatattat ggggctaggt cagtttatgg aagtgagatt ccacttgctt
    17761 gtgtcggtat cattattaaa gtaaatatga tttttatatc tataattatt ggtatatcac
    17821 aaggtatgca accaattata gggtataatt atggggctga aaagtataat cgtgtaaaag
    17881 aagcatataa gtttgcagtg ggaagtgcaa ctattatatc tgttattgca ttcttatgtt
    17941 tccaattctt cccaagacaa attacgtcta tatttggtaa cggaaatgag acttatttta
    18001 tatttgcaga acattatttt agaactttct tgttcttaac atttataaat ggtgttcaaa
    18061 tagtatcatc aactttcttt acatcaattg ggaaatcagt gttgggactt tttacttctt
    18121 tgactcgtca aattttattt ttaattccat tgatagtaat tttaccaatg tttatgggaa
    18181 ttgatggagt aatgtttgca ggaccaatag cagatgcagc agcggcaatg gtatgtatat
    18241 actttgcagt tcatgaaatg aaatcattga ctataaagca aaataatttg atgagtaacc
    18301 ataataagat tgcagaggta taagacaata tttaagaagt atagaaaatt aatttaaaaa
    18361 atataataaa ttaatttagg aagtgtagaa aattaatcta aagaagtata ataaactaat
    18421 ttaggaagta tagaaaatta atctaaagaa gtgtaataaa ctaatttagg aagtatagaa
    18481 aattaattta aagaagtata ataaactaat ttaagaaata tagaaaatta atttaaagaa
    18541 atacaataca ttaattttta agtaacatta aattaatgta agtaaaataa tgttcatatg
    18601 aatatttaaa ggtgcatttt ctgaagctaa gtatatgttg tagagtgtta tgtctctcta
    18661 tcctgtgaag tttaatagct caagaaaatg cactatttat gatgattatt ttgttttatt
    18721 tccaattttg gaaataacta ttctaattaa atctaaaatt ataccataaa taagaaaaga
    18781 aagtatagat gctatgtacc aataaatcga aattgaccct gtaattgttc tacatgggat
    18841 ttgcatacct gaatccataa aaacaaaaaa actatcaact ctggaaataa tagacaaaat
    18901 aggatttaaa cctattagaa caatactttt tgagtcttga ccaatttgat gcatataaat
    18961 ctcaaataat gcaattaaaa aaaaccaaga tgtaaaagat ttaataattt ttttcatcaa
    19021 aatacctcac ataatataaa ttctgtttgc actatttgct tactacaact atttatctcc
    19081 attcagtcac atcacttaat tgtaatctag gtcttcttga tggttcttca tcaggataac
    19141 ctaaagatat tgctgccact aattgactat ctgtatttaa ccattcacat aattcacgat
    19201 atgcaaagta tacatcacaa atccaaagac tacctagacc taattccaca gctgtaagcg
    19261 acatattctg aattgcagct ccaattgatt gcatatttgc catttcatag aatcgttctt
    19321 caggggatag cttttctaaa ggtgatttac ctaatatatt taaaataaaa attgttactg
    19381 gagcttgttt catgatttca actgtgtaat ttgcaccagc gatatgttga cgacttcctg
    19441 gaagaagccc attatcatta atttcatttt gaataccttt gctcatggct ttaagcatag
    19501 attctttttc cttttcagtg atgacaacaa aacgccatgg ttgcctgttt ttagatgaag
    19561 gtgcatttat tccagcttca attattttct ctatagtttc atgtgaaatg ctttgatttt
    19621 tatattttct gatacttcgt ctttttgata tagaatcact tatcatttta agtaacctcc
    19681 gtttattttt tggaaaaatc tatttaattt ttattttcta ttttaattat tttagcatta
    19741 atcagaaaaa aagtcattga ttttagtata caattattaa tgattatatt aagaattgta
    19801 tcaaaaagta tgtgattata ctaagggatt atattaaaca ttatatttaa aattgtatga
    19861 aaggttgtat ttaat
//
LOCUS       DAYATN010000062        19560 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.062, whole genome shotgun sequence.
ACCESSION   DAYATN010000062 DAYATN010000000
VERSION     DAYATN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.062"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            149..766
                     /locus_tag="ACGI8A_002137"
     CDS             149..766
                     /locus_tag="ACGI8A_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HFH0180134.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(923..1375)
                     /locus_tag="ACGI8A_002138"
     CDS             complement(923..1375)
                     /locus_tag="ACGI8A_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HFH0180135.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1823..3181
                     /locus_tag="ACGI8A_002139"
     CDS             1823..3181
                     /locus_tag="ACGI8A_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0180136.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            3454..3834
                     /locus_tag="ACGI8A_002140"
     CDS             3454..3834
                     /locus_tag="ACGI8A_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0180137.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            3959..4159
                     /locus_tag="ACGI8A_002141"
     CDS             3959..4159
                     /locus_tag="ACGI8A_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180138.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            4355..5083
                     /locus_tag="ACGI8A_002142"
     CDS             4355..5083
                     /locus_tag="ACGI8A_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180139.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            5105..6022
                     /locus_tag="ACGI8A_002143"
     CDS             5105..6022
                     /locus_tag="ACGI8A_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HFH0180140.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            6164..7348
                     /locus_tag="ACGI8A_002144"
     CDS             6164..7348
                     /locus_tag="ACGI8A_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0180141.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTYLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            7455..7643
                     /locus_tag="ACGI8A_002145"
     CDS             7455..7643
                     /locus_tag="ACGI8A_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFH0180142.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            7792..8442
                     /locus_tag="ACGI8A_002146"
     CDS             7792..8442
                     /locus_tag="ACGI8A_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFH0180143.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            8545..9483
                     /locus_tag="ACGI8A_002147"
     CDS             8545..9483
                     /locus_tag="ACGI8A_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFH0180144.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            9605..10360
                     /locus_tag="ACGI8A_002148"
     CDS             9605..10360
                     /locus_tag="ACGI8A_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0180145.1"
                     /translation="MKKYNISENGALIKNDSIESKSESVKVVQISDTQIGSFYSTKNL
                     KKVANKINTLNPDIIVFTGDLIDYSNKNPSVDEITTILSSMNARLGKFSVFGNHDYMY
                     KLPRYYRQIMKNSNFNLLVNENKKIKLKDDKYINILGVDEILNGNPNIKYLESQIDNK
                     SFNLLLAHEPDLVDMLSKDTMNLVLSGHSHGGQIRLPIKGALVTPPYGRKYTKGFYDI
                     NGNHLYVSSGLGSTKLPFRFFNIPEIIEFNIII"
     gene            complement(10645..11379)
                     /locus_tag="ACGI8A_002149"
     CDS             complement(10645..11379)
                     /locus_tag="ACGI8A_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFH0180146.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            11504..12220
                     /locus_tag="ACGI8A_002150"
     CDS             11504..12220
                     /locus_tag="ACGI8A_002150"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFH0180147.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            12354..13046
                     /locus_tag="ACGI8A_002151"
     CDS             12354..13046
                     /locus_tag="ACGI8A_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0180148.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            13036..14217
                     /locus_tag="ACGI8A_002152"
     CDS             13036..14217
                     /locus_tag="ACGI8A_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0180149.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            14499..14954
                     /locus_tag="ACGI8A_002153"
     CDS             14499..14954
                     /locus_tag="ACGI8A_002153"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFH0180150.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            14944..15669
                     /locus_tag="ACGI8A_002154"
     CDS             14944..15669
                     /locus_tag="ACGI8A_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HFH0180151.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            15641..18022
                     /locus_tag="ACGI8A_002155"
     CDS             15641..18022
                     /locus_tag="ACGI8A_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HFH0180152.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(18227..19456)
                     /locus_tag="ACGI8A_002156"
     CDS             complement(18227..19456)
                     /locus_tag="ACGI8A_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFH0180153.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
BASE COUNT         7825 a         2048 c         3384 g         6303 t
ORIGIN      
        1 tatatttttt taaggatata aagtaaaaaa atatacaata tcaagaaaaa aagatattgg
       61 gtatttaaag taatacttta tttaatttta agtaaatcgg gtatctacat atatagatac
      121 taatttcaaa gaataggagg gtgtttatat ggaaatattc gcaataggac attcaaatta
      181 tccatatgat aagcttatta atatgataaa aaaatatgac ataaattgtg tagttgacat
      241 tagagaaact ccatattcta aatataatat tcaatacaat aaagaggcat ttaacgaatc
      301 tcttagaaat tccggatttt tatatatata catgggaaaa gaatttggag ctaaaagaac
      361 taataaagat gtatatacgc aagaaggtta tgcagatttt gagaaagtag caaaagaaga
      421 tattttttta aacggaatag aaagattaaa aaagggttgt caaatgggtt ataggatagt
      481 tttattagga gctatgcaag aacctataag atgtcataga agtatcttgg tgggaaaagt
      541 attaaataaa gaaggttttg atgtaaaata tataatgcat gaaggaaatt tggcatatca
      601 ggaagatata gaggaatcat tattggataa gtattttagt gatagaaagc aactgagtat
      661 tgacaattta ttagggagtg cattaacgag agaagaaatg atacaagaag gttataagct
      721 agctaataaa gaaataggat atagaactga gaaactaggt aaataaaaat atacaaatag
      781 ataaaataca gtacttatct taagtaaaat aatattatga atttataata gctaggcatg
      841 atgtgccaat taaaaatatt ctagttattt aataatatca ttaaatttag ggtatttttt
      901 aatagcacat catgttgcag ttttaaaaaa taaaatctct aatattgcca taatcaactg
      961 ttctattaag agtaggatga ctagatataa atctaactgt aatttttgaa tttaacaaac
     1021 aaggatttga aattaaaaat actctatctc cattgacaat agaagtaata gctactggca
     1081 aaatatctac aaatgtatca tatctgtttt tagcaatttg ataaggataa tttcctacaa
     1141 aaacaaatag atttaaatca tatatattat cagtaaacca agcccattga ccataaactt
     1201 tgtcaatatt cattgtgcct aaaccaagtg tatcaaagta tgttgctata gttaaataaa
     1261 tattttccat gttttctaaa tatgaaacag tatatcttct gaaacgtatg ggtgctgtgg
     1321 aagaaatagg ataaaccata tttacattta gattatttgc atttaaatta accaaagtat
     1381 cacatcctaa aattagtcta taaaatacaa tatttaaatg gatgcgattt tgttacatct
     1441 aaagtgtaat attttatata ttgaaaaagt ggtaaaagtt tggatgatta aataatatta
     1501 taaaaaatat ataaaatgta cttattgtta ttgctacacg atagtgatac aatattaacg
     1561 aaatatagct acttttaata ataacggaaa atatttatat taaattaata aatattttaa
     1621 atgtggtatc cataaaaaat aataaacata agataattat aaagatatac tgtgttcaaa
     1681 tgatattaaa caagatttat attatttgaa agcagtatat tataatgcga acaatacaat
     1741 tctaaacttt gcattgtcaa tttacagttc gataaactaa ttaaaaatta aatttatata
     1801 aagagggaaa agaggaatag ggatgtttat aaggaaaaat gttataaagt tagcaattcc
     1861 tataatgata gagcagacgt ttgtcatgct tttaggagta tttaatacaa tgatggcagg
     1921 tcatattggg gaagaagctg tatctgcagt tggaatggtt gattcaataa acaatatatt
     1981 tatatctgta tttgctgcac tatctgttgg agctactgtt gttgtagctc aacaaatagg
     2041 aaaaaaagat agtgaaaagg tgaatgaaac tattaagcag gctctagtgt ctggagtaat
     2101 attatccttt acaataacaa tattgatgtg ggtttttaga actggtgtaa ttaatttatt
     2161 ttatggttcc gcagaagaat tggtaaaatc aaatgcaaaa ttatacatag agtttacatt
     2221 atttacgtat ccatttattg cgacacaaca aatagcaaat ggtattttaa gagggtgtgg
     2281 agatgtaaag aaacctatgt atgtgactat gtttatgaat atagtaaatg taacattggg
     2341 atatatactt atatatggta ttgatttaaa tcctctagga ataaatttgc aaacaccatc
     2401 ctatggcata acaggtgcag ctatatctat tgctatagct agaataattg gatgtatagt
     2461 aattataaga gctttattta aaggaaacga atttatatct atgggtaaaa tatttccatt
     2521 taagattgat gttgaaactc aaaagtctat atttaatata ggaataccag ctggagttga
     2581 acaactactt tttaatgcag gtaaattgat tgttcaagta tttatagtta ctatgggaac
     2641 atcagcaatt gcatccaatg caataggaat gtcaattgca tccataataa atgtaattgg
     2701 gaatgcttta gctttgtctg ctacaacctt ggtgggacag tatataggac gtggagatat
     2761 taaaggagct aagagtactt tattatattt aactaaattt gcaacaattt gtttatttat
     2821 agtaggagct atatttatac caattgcacc ctggatttca agtatgtata ctcaaaatgc
     2881 tagtgtaatt gacatctcaa cacaattaat tcgaagtgat tgtttagcta tggtaatatg
     2941 gcctatatcc tttgtattat cagcagggct aaagggagcc ggtgatacta gatatacaat
     3001 gatgactgca gtaataggta tgtggatttt tagaattttt acaggttacg tattgggggt
     3061 tgtattagaa tttggagtgt taggaatttg gataggtatg tatactgatt ggcttgttag
     3121 agggacttta tattgtttaa gattaagagg aacaaagtgg ctaaaacaca aagttgcata
     3181 gaatatatga aatcactttt agatggataa ctctatttag aagtgatttt ttatgtagtt
     3241 attatcataa ttataagtat gatatataag gtgattaaat tacataaata aaggctataa
     3301 tagtatggat attataaatt atatattgag cagaattaca taatttggcg ataaatatta
     3361 gaaatatata tctatagaaa ataatatata tgggtatatt attaaatata aaaaatatta
     3421 ggtatttatt taataataaa ggtgggtatt gttatgataa ataaaatagg aaaaataact
     3481 ctctatgtaa ataatcaaga ggatgcaaag ttattttgga cagaaaaatt gaactttgta
     3541 gtaacttatg aaaaggaaat gggtccaaat atgaaatggg tggaagtagc accaagtaaa
     3601 aatgaattta caacatttgt gttgtatgat aaaaatctaa tgatgaagca aaacaaagag
     3661 ataaatgtat ctcatccatc aataatttta agtacaagag atattaatgg aacttatgaa
     3721 caaatggcaa ataacgaggt tgaggttggg gaattaatgg agatgccata tggaaggatg
     3781 ttttctttta aagaccaaga tggaaatgat tatttggtca gagaggataa ataattttac
     3841 aaagtattaa atttactcaa tatctgatat aattagagac aataattatt ataaactagt
     3901 ctaggattag aattatataa ttaaaaatta agtgtaaatt ttgagtagag gtgataaaat
     3961 gtttaagaaa atggcagtac taaaagatat agcaactaaa ataggtcgta aaaaagcgta
     4021 tgaactatta gaaatggttg aaggtaatga tgcctttgta gctgaggtaa agataaaaaa
     4081 gaatggaata gaatctaaaa aagaagaaat tatgttaaaa gataatcaaa aaataatatt
     4141 agagtatata gaaggttaaa ttttaaatac atataagttg atatactaaa tttctgagat
     4201 gagtttacat aaaactcatc ttttttactt ttcaataact ttgttttatt atttaatata
     4261 cattttatct ccttagagtt gctgatatat aaaaattact gatataataa aagaatggat
     4321 attaatgtaa tattttatag aaaagagggg tattatgctt gaaataaaaa atctttcctt
     4381 taatgttgag tctaacaatg aggaactagg aataataaat gatgttagcc tttcatttga
     4441 gaggggaaaa cttattgtta tcacaggtcc aaatggtgga ggaaaatcta caattgctaa
     4501 gcttattatg ggaattgaaa aagctacgag tggtcagata attttagatg gagaagatat
     4561 aacaaatctt agtattacag aaagagcaaa aaaaggaata ggatacgcat ttcaacaacc
     4621 accaagaata aaaggtatga cagttgaaaa tttacttaca cttgctcatg gaaaaccatt
     4681 gtctactgat gtatgttgcc aataccttac agatgttgga ttgtgttcaa aagactattt
     4741 aaatcgtgaa gttgataact ctctttctgg aggggaaatg aaaagaattg agatagcaac
     4801 tctttttgct agagatttaa aggtttctat atttgatgaa cctgaagcag ggatagacct
     4861 ttggagcttt ggaaaactca atgaaagttt taagaaaata catgaagagt ctaatcagac
     4921 tataatcata atttctcacc aagaaagaat attagagtta gctgatgaaa taattgttct
     4981 acaagatggt tcagtaaaaa gccatggaac aaaagaggct atattacctg aaatcatgtg
     5041 tcaagttaat tcaagttgtg aattaatgaa agacatgaat taattagaaa ggaaggcaaa
     5101 ttaaatgaac aaaatagatt taaatctttt agaaaaagtt gctgaacttc gtggaactcc
     5161 attaggagca tacaatatac gtaaaaatgg gcaaggagta gagagacatt caactgaaaa
     5221 tgtaactata attcctaaaa ctgataaacc tggaatagat attatagtaa agccaaatac
     5281 aaaaggagaa aatgtacata taccagtaat agtaacagag gctggtgtac aggatatggt
     5341 atataatgat tttgaaattg gagaaaatgc agatgttgtt ataattgctg ggtgtggtat
     5401 acataactgt ggttgtgatg atagtgaaca taatggtata catagatttt tcttacgtaa
     5461 aggagctcga ctaaaatatg tagaaaaaca ttatggtgaa ggtgatggag aaggaaagag
     5521 aattttaaat ccagttacta ttgtcaatat ggatgaggat tcatattgtg aaatggaaac
     5581 aattcaaatt aagggtgtag attcaactaa aagagaaatg gatgcaaacc taggtaaagg
     5641 agctaaaata gttgtatttg agaaattatt aactcatggt aatcaagttg cagaatctaa
     5701 tatgaaggtt atcttagagg gtgaaaactc ttctgctcaa gtagtttctc gtacagttgg
     5761 gcaagataat tctaagcaaa agtttaatcc ttgtgttatt ggggattccc tttgttctgc
     5821 acatgttcaa tgtgattcaa taattatggg aaattctcaa attagtgcta tacctgaaat
     5881 tgctgcaaat catccagatg ctcttttagt acatgaagca gctattggaa agattgcagg
     5941 agaccaaata ctaaagatga tgacattagg tcttactgaa gaagaagccg agcaacaaat
     6001 acttaattgc ttcttaaagt aaattaaaga ttttatttaa tttaatataa ttaatatagt
     6061 ataatatagt gaataagtct aaaatatttt tatttttagg cttattttct ttatgtaaga
     6121 aaagctatta gagaaattat attgtaatta ggatgtggaa taaatgaaaa atttacttat
     6181 aagaaatggg acaataatat ctccaacaga tggtatcaaa tttgaaaaaa gagatatctt
     6241 ggttttacat gggaaaatat atgctattgg attgaacctt aaagagaaaa taagaaaaga
     6301 aaaattactt acaaataaag agttcgagat aataaatgcc gataatatgt atatttcccc
     6361 aggatttata gatgttcaca cccattgtta taagagaaaa ttacctacag gtatggattc
     6421 tgattcaatt ggaattgaaa aaggttctac agttatattt gatgcaggta cagctggtcc
     6481 ctcaaactac tatgatttta aaaaaaagta tatggatgag tgtaaaacag aagtatattc
     6541 ttttttaaat gttacaaatg agggactaaa tatattgaat gaatcaactt atttagatgt
     6601 tataaatttt gacaaggttg agtcaattgt agaaaaaaat atagaaaaga taaagggtat
     6661 aaaagtaaaa gcgagtaaat ttcaatctaa tgaaagtggt atagatataa ttaagaaatc
     6721 aaagcaagtt gctaaaaaat taaatttacc cttagtggta tatataggag agtatccatc
     6781 atatatagat gaagtaatga atattttggg aaaaggagat gtggttacac cggtatatgg
     6841 aaatagtaca aatggaatcc ttaaagaatc tggcacatta agaaaatcag taataaatgc
     6901 aaagaataga ggagtattgt ttgatgtatg tcatggagtg gaccaatttg attttaaagt
     6961 ttttaaaaaa gcaataaagc aaggtttaga gcctgaccta ataagtactg atatgcatat
     7021 aaaaaacatg aaagacgtaa actattctct tttaaatgtt ataaataaaa ttatggaact
     7081 tgggatatcc ctagaaaagt gtatagaaaa agtaactgaa tacccaacaa agattttcga
     7141 tttacatgga aataaaggca gaataaagat tggtggagaa ggtgatttta caatatttac
     7201 aatggaggag tgtaatgata ttataaaaga ctacaataat aatgaacttg ttttaaataa
     7261 aaaattaaga ttgcaatata ctgtaaaatc atggatgaaa agcagtgaag tttatagaca
     7321 tggatacgaa aatactataa taaactaaac aatagtgata aaataataaa tatatgttta
     7381 aattggtata tattggataa atatctaagg aaacaacaat taaaaaataa taattttagg
     7441 ggaggtattt tagtatgaaa gcatttgttg ataaggatgt atgtgtagct tgtggagcat
     7501 gtataggaac atgtccagac gtttttagta tgagcgatga agggcattca gtagcaattg
     7561 aaggagaaat accagcagat ttacaagatt tatgtaaaga agccgaagaa ggatgtccag
     7621 tttcagctat aacagttaaa tagagctgtt taaatttatt tttaaaataa atatataaga
     7681 tttaattggt acttaagcta tagtattaga aatttctatt actatagctt tttaaaatag
     7741 ataaacttaa tatataagat attacttttt acaaatatgg aggactgaaa tatgaaaaaa
     7801 aattatgaaa tagtattatt cgatttagat ggaacactta cagaccctaa agaaggaata
     7861 acaaagtcta ttcagtattc tttaaatagc tttggcataa aagaagattt ggaaaactta
     7921 gaccaattta taggaccacc attacatgat actttcaagg agtattataa atttgaagat
     7981 aaaaaagcta aagaagctgt tgaaaagtat agagaatatt ttgctgataa agggatattt
     8041 gaaaataaaa tttatgagaa tatgaaagag atattagaaa tgctctataa aaatggtaaa
     8101 atattattag tagcaacttc taagccaaca gtttttgcag aaactatact tagatatttt
     8161 gatatagata gatattttaa atatatagca ggtagcaatc ttgatggtac aagagtgaat
     8221 aaaaatgaag ttatacaata tgtactagac ctatgtaatg taaaagataa agataaagtt
     8281 attatggttg gagatagaaa gtatgacata attggagcta aaaaaatagg tatagattct
     8341 attggagtat tgtatgggta tggttctttt gaagagatta gtgaaagtga acctacttat
     8401 attgtcgaaa atgtagagtc tataaaagat attttgttat aaatatcttt tatagacaaa
     8461 aatattacaa aatggtgtcg catatttaaa aatatgatat tatagttaag gaagtcattt
     8521 aaaaaagagg aggatgcact taaaatgaaa tctattttaa ttatgggtgg aagcgacttc
     8581 ataggttctg cacttgctaa gcgcttgatt aaatgtgggt atcaaattga tatacttaca
     8641 aatggtaaaa aggaaataga ttataatggt tttaaagaac atctaatttg tgatagaaaa
     8701 gttcgaaaag atatggaaaa cattatcaca ggaagaaaat atgattacat atatgatatg
     8761 acagcatata caaaagaaga tgtaagtaat ctgattgatt ttataagcat ggataactta
     8821 aaaaagtata tagtattgtc agcaggagca gtatataaag atagtggaag aaatataaaa
     8881 gaagaaaatg agaaaggtga aaatgaaaac tggggaaaat atgggttaaa taaaaaagag
     8941 gctgaagatt ttattataaa tagccctata ccatatataa ttattagacc aacttacata
     9001 tatggtgaaa ataataattt atatagagag tattatttct ttgaaaaaat agaaaaaaat
     9061 gaaaagatac cagtaccaaa aggaaaacaa gttagtaatc aatttatata tattggggat
     9121 ttggtaaagg tattggaaag cataatgaaa aatcctcatg taagagaggc ctataatgtg
     9181 acaaatccac agttaatttc ttgggatgac cttatatata catgtggaga aattattggc
     9241 aaagaaccta taataaaata tgtagatatg gaaaaagtag agtttagaga aagaacgtat
     9301 ttcccattta gaaatataga ctttaatctt gatataaata agcttattga acatgggctt
     9361 tatataccaa acgtgttact aaaagaaggt cttacagcaa catataaatg gttttcagca
     9421 aataagccaa aaatgcatga cagaaaaatg aacaaagttg agtctgtaat gcaaatagtt
     9481 taaaaaaata aataagtagg tagatatttt gaactcaaaa attaagtatt ttttaaaatt
     9541 ttttatctta atatttattt tgatttttat atactcagtt gcgatagagc cgagtctatt
     9601 aattgtgaaa aaatataata taagtgaaaa tggagcattg ataaaaaatg attcaattga
     9661 gagtaaatct gaaagtgtaa aagtagttca aatttcagat actcaaatag gtagttttta
     9721 ttcaactaag aatctcaaaa aagtagctaa taagattaac actttgaatc cagatataat
     9781 agtatttact ggtgatttaa ttgattactc caataaaaat ccaagtgtag atgaaattac
     9841 aactatttta tcaagtatga atgctaggtt gggaaaattt tcagtatttg gcaatcatga
     9901 ttatatgtat aaacttccta ggtattatag gcagattatg aaaaattcca acttcaatct
     9961 tttagtaaat gaaaataaaa aaataaaatt aaaagatgat aaatatatta atatattagg
    10021 tgttgatgaa atattaaatg gaaatcctaa tataaaatat ctggagagtc aaatagataa
    10081 taaaagtttt aatttattgt tagcacatga accagattta gtagatatgc ttagtaaaga
    10141 tacaatgaat ttagttctat ctgggcatag tcatggtggt caaataagat tacctattaa
    10201 gggagcatta gttactcctc catatggaag gaaatatact aaagggtttt atgacataaa
    10261 tggaaatcat ttatatgtga gttcgggtct tggaagtaca aagttaccat ttagattttt
    10321 taatatacca gagataatag aatttaatat tataatctaa cataaaatat ttagaaaata
    10381 taattttgct ttttataata tgtagatgta tatactgtaa aaatggatta aaccagtaga
    10441 ttgaatgaat taatagatta aattaatata taaaatattt aagttataat taatgtatta
    10501 gacaaaataa atactattaa gaactattaa aatatagatt aaagataata acatataata
    10561 aataaaaaag taagattgtg tagattatat aaagattatt tacaattaat acaatcttgc
    10621 ttttttattt aaaaatttta attattattt aaaatttttt atcttctctt tatcccatat
    10681 aaacaatttc tcagaattat ttaaagcacc aaatacattt cttttaaaat caggcatctc
    10741 tttatttaaa ttagcaataa gattttttat gtactctctc ctagtatgac catttgcagg
    10801 gcagggattt gtgataatag gatagttaga atcttttgaa attttcttag tcatatactc
    10861 ttctatgtaa accataggtc ttataactgt taaatcctgt ctgtctaggt atgtctttgg
    10921 ggaaaaacaa tttactctac cctcataaaa catagacatt aaaaatgtct caattgcatc
    10981 atctttatga tgaccaagag ctactttatt acatccaagt tttttagcat tatcgttcaa
    11041 agcacctctt cttaaattag cacacaatgc acaaggattc ttttcttgtc ttatatcaaa
    11101 tacaatttct tttatgtcag tttgaaattc ataaaaagga acatttattt ctttacacaa
    11161 tttatctagt ggagaattat caacacctcc agtatttaat gttatggcta tcaattcaaa
    11221 gttttgtgga gaaaatcttt gataactttt aagtatatga agtagagtta aactatcttt
    11281 tccaccagaa agtccaaccg caattttatc attttcttgt atcatatcaa aatcttgtat
    11341 agccttacga gctttactta gaagtttttg tatagacatt ttatcaccat cctttaaaaa
    11401 tattaaattt aagatttatt taagatttac ttataaaata cttaagtctt atactctaaa
    11461 attaacatta tagcaaacat aacaaaaagg agaagattta attatgaaaa aatttaaatt
    11521 aataggagcc attattttag tagtatttat aagtgtattt atatctaaag atagattact
    11581 taagaaagat aaagatccaa tagtagctgc tcaacatgat tcagaacaag atgttgaggc
    11641 aaattctcaa aataaaaatc actcaaatag tatatcaaat atattattgg taaacaagac
    11701 aaatggaatt agcaaaaact atactcctga aaatattact aaggtaaaca taccttttgt
    11761 tgaagaagca acagaggaag aaaaacaaat ggcaggagaa cctgcaaagg cagttgaaga
    11821 tttagttaag caagctaatt cagaaggaat acagttttta gggtcttcag catacagatc
    11881 ttatgatact caactggata cttatacaag aagggtaaaa tctcaggggc gagaaaaggc
    11941 agatgcgtat gtagcaaagc ctggttacag tgagcaccaa actggacttt gtattgattt
    12001 aacaaatcca gaaagatggt ttgttggaag tacaaaagaa gctaaatggt tggcagaaaa
    12061 tgctcacaag tttggattta taataagata tcctgaaggt aaagaagata taacaggtac
    12121 agcatatgaa ccatggcata ttagatatgt tggtaaggat gctgccgagg aaatttattc
    12181 aaaaggatta actttagaag aatatttaca aaataaataa tataaatgtg taaataattt
    12241 gtattatatt ggaaatttag aaattaataa atttatagat tacactaact aaaatatagt
    12301 ataattttat atgaataaag ctatatacaa agattaagtt aggatgtgta aaaatgaata
    12361 atacagttct tgttgtggat gatgaaaagg aaataagaga ccttatagag atatatcttt
    12421 taaatagtgg atataatgta attaaagctg aagatggaaa agaggctcta gatattttaa
    12481 aaaaggaaaa tattcattta atgatattgg atataatgat gcctaaaatg gatgggatag
    12541 aagtttgtag aaaagttaga gaaagtttga atataccaat acttatgtta agtgcaaaat
    12601 cagaagacat ggataaaata caagggataa tgactggtgc agatgattat ttgacaaaac
    12661 catttaaccc acttgaactt actgtgagag tgaagtcgct gattagaaga gcatattatt
    12721 ttagtggtgc taataatgaa gataaagatg tcataaaggt tggagtagta attatagata
    12781 aggaaaaaca tagtgtaaca gtggctggaa atgaaataat tttgacatca agagaatttg
    12841 atatattata tttactagca aataataaag gcagagtata tagtacagaa gaaatatttg
    12901 aaaaggtatg gaaagagaaa tattaccaat ctaataatac tgtaatggtt catatgagca
    12961 gaataaggga taaaattgaa aaatatacag atggagaaaa aataattcat actgtatggg
    13021 gagtaggata taaaattgaa aaataaaagt ttattaaatc atatgtttcg acccatatta
    13081 gatcttataa taagctgtgc tttaacttca atgcttattt taacaagtat gtatataata
    13141 aaatcattat atgtcaatag tgatatgtct gtagttaatc aacttgtata tcaagcaaga
    13201 gatttcagaa gtcttatagg gtatgattta ttttatccat ttttatttac aatcacatca
    13261 ttagcaattt attccatgat atcgtacaag agaaataagc aaattgtaga ttttataaga
    13321 aagactaata gaaaaaatca aaaattagaa atcattaaaa gtgctgtaaa tttaatggat
    13381 gaaggtaact tagaaaaaag tatagatatt gagtatgatt tagatatatt aaaggataaa
    13441 gaaaaggatg atattgatga actagcacat aaaatcaata atatagtcca tcaacttaga
    13501 aatataacta ttgaagagag acaagctcag cagacaaaaa cagatttaat aacaaatgtg
    13561 tcacatgact taagaacacc tttgacatcc ataatgggtt atttaggagt gatagaagaa
    13621 ggtaagtata aagatgaagt tcagatgatg tattatgttg atattgcata tgaaaaatcg
    13681 aaaaatttaa atgttcttat aaatgatttg tttgaattga ctaaaatgca aaataataca
    13741 ataaaattaa acaaaataga aataaattta gtagagttat taagtcaagt agtatctcaa
    13801 tttgaaattt attttaaaca agaatttatg gttagtagaa ttaattttaa tgaagaaaaa
    13861 ttaattgtaa aagctgatcc aaataaattg gtaagagcat ttgaaaatct tataactaat
    13921 gctgtgaaat atggaaaaga tgggcattat gtagatatag tcacagagaa aaaggaagat
    13981 atggcggttg taaaagtgat aaattatggg gaaccaattc cagtgttaga tttaccaaat
    14041 atatttgata gattttatag agttgaaaaa tcgagaaata gaaatgttgg aggttctgga
    14101 ttgggtcttg cgattacaaa aaatattgta aatttacatg atggagaaat tactgtttca
    14161 agcgataaat atcaaactgt gtttgaagta caactgccaa taatacaatc agcttagtta
    14221 taaaataaaa gtacgattat gtgatataaa tattataaca ataaagtgtt tacaaaaata
    14281 aaaaagtaat atataattta agtcagttta ataagatact tatcaagagt taggggagag
    14341 agttagctca gtgatcctat accaaccttt tatcaggtgg tcctgctaac aacgatgaga
    14401 tgacttgaaa taatatactc ttcttatcaa taatagatag gaagagtttt ttttagaatt
    14461 aatgtaacaa attttaaagt ataatggagg attaaataat ggaaaaagta gaaagtttta
    14521 agctagacca tacaaaagta aaagctcctt ttgtaagaaa gtgtagtgta cttgatggag
    14581 taaagggaga taaagtaact aaatttgact tgagattttt acagccaaat gtagaaagtt
    14641 ttggaactgc tgctatgcac ggtttagagc atcttttagc tacttattta agagatacat
    14701 tagatggtgt aatagactta tctccaatgg gatgtagaac aggattttat ttaatactat
    14761 ggggagatgt agatgccaaa acagttaaga ttggtttaga agaagcatta aaaaaagttc
    14821 ttgaatcaga taaaatgcca gcagctacag ctatagaatg tggaaactac agagacttat
    14881 ctttatttgg ggcaaaggaa tatgcaaaag atgttttaga taaaggattt tctttaaata
    14941 tatatggaga ataatttaat tggaagagaa gatataagca taaatcaaag tgaagtttta
    15001 agatatttac aatatagagg tcaagagata gataaagacc ttaaatatac tattgatgaa
    15061 tgtgtagcta taactaaaaa taaaataaat cctagatatt tatctaggat ttatcctatt
    15121 aaaataaata aagataatgt tgtagaatta gagggaacaa atttaacatt agagagtaaa
    15181 gatgtatatg aacttttaaa agaatgtaat gagtgtgtaa ttatggcagc tactataggg
    15241 atagatatag aaaaagaaat aagacgacac tcttattcaa atttaacaaa aggactaata
    15301 attgattcat gtggaacaac agcaatagaa gaagtgtgtg acattgttca aaataattta
    15361 gaaaaggttt ttttaaagca agataaattt ataactatgc gatatagccc aggatatgga
    15421 gatttaccaa tagaaaaaaa tatagatata ttaaacgttt tgaatgctca aaaacaaata
    15481 ggtctaacaa ttacttctaa tggaattatg ataccgagaa agtcagtagt ggctattata
    15541 ggtatttcta atacaagaat aaataaatct aagaaagaaa agtcgtgtga aaattgtaaa
    15601 aactatagaa actgtacata tagaaaagga gcgaactctt gtggaaataa gacaatatct
    15661 gaaaaataat atattaatat ttgatggtgc aatgggaaca atgcttcaac aaaaagggtt
    15721 aaagttaggt gaaaatccag aagtatttgg tcttcaaaat ccagataaac ttatagaaat
    15781 acatacagca tacttagagg caggttcgaa tgtaattctt acaaatactt ttggatgtaa
    15841 tgaactaaaa ttagatagta agtatactgt agaagaagtg attgacaatg ctgttttggt
    15901 ggctagaaaa gctatagaaa atgtagataa tactaaacca agatatgtag ctttagatat
    15961 tggtcctata ggagaaatgc tagagcctat gggtacttta agttttgata aagcatatga
    16021 aatatttaaa agacaagtac tacaaggcgt taaatctgga gttgatgtaa tagtaataga
    16081 gactatgatg gatttatatg aagctaaagt agctgtatta gcagcaaaag aaaactctga
    16141 tttacctatt ttctgtacta tgacatttga tgaaggtgga agaagtttta ctggatgtat
    16201 gccagaatgt atggtggcta ctatagaagg tctaggtgta gatgcaatag gagttaattg
    16261 ttcattagga cctaaacaac ttttaccaat agttgaaaaa atagcatcaa gagctacagt
    16321 ccctgttatg gttcaagcaa atgcaggtct tccaaatata gttgatggag aggctatata
    16381 tgatgttgat gcaaaagaat tttttgaagg ggttaaaaag ttcgttgaag ttggtgctac
    16441 tataataggt ggatgttgtg gtacgaatcc aagttttata aaagaaatat ctgagaatat
    16501 aaattctgtt acaaagggat gtatcgagaa aattgataaa tgtgtagtct gttcaccatc
    16561 aaaatttgtg gaagtacaat cgcctacagt tgttggagaa agattgaatc ccacaggtag
    16621 aaaatctcta caagaagcat taaaaaatga aaatgttgat tatgctataa atttaggttt
    16681 agaacaagta aatgctggag cacaaatact tggagtaaat gttggtcttc cagagattga
    16741 tgaaaaaaaa ctaatgccaa aattaataag agagattcaa gcagtagtag atacaccttt
    16801 acaagttgat tcttccaatg tagaggcctt agagcaagga cttagatatt ataatggtag
    16861 aacaatagta aactctgtaa atggcaaaga ggagtcttta gaaagtatat taccaatagt
    16921 taaaaagtat ggttcatgtg tggttggact tacacttgat gaaaagggga tacctaaaaa
    16981 ggctgaagaa agatttgaaa tagcaaaaag gatagttaat agagcagttt cttatgggat
    17041 aaaaccaaaa gatatattta tagactgttt atcattaact gtttcagcac aacaagaaga
    17101 agcaatagaa actataaaag ctataacaat ggtaaagaca ctaggagtaa aaactatatt
    17161 aggtgtttca aatatttcat ttggtcttcc aaataggaaa gcacttaatg cttcttttct
    17221 aacattagct ttaggggcag ggcttgattt agcaattata aatccaaatg aatatagtat
    17281 gatggaagct atcaattctt ttaaaatatt aaataacaca gataaaggat gtataaacta
    17341 tataaatcaa tatagcaata taaataattc aaaaaaatcg gattcatcta caaagattga
    17401 taaagatttg cccttggata ttttagttga aagaggatta aaagatgaag caaaaaatgt
    17461 aacactgaat cttttaaaag agaaggatga aaattatata ttagatgaga ttttaattcc
    17521 tgctcttgat aaagtaggaa aaagatatga tagtggagat atatttttac cacaattgat
    17581 tcaatcagca gaaacggtaa aagtttcatt aaatacaata aaggaaactt tattaagtaa
    17641 aagtagcaat aatgtatcca aaggaaaaat aatagttgct acagtaaaag gtgatatcca
    17701 tgatattggg aaaaatatag taaaaataat gctagaaaat tatggatatg aagttataga
    17761 tttaggtaaa gatgtcccta ttgaagaagt agtggaagtt gctaaaaaga gaaatataaa
    17821 attagtagga ttaagtgcac ttatgactac gactgttcaa agtatgaaag atacaattca
    17881 ggcactgaga gataatgaga taaatgcaaa ggtatttgta ggaggtgcag ttttgacaga
    17941 agaatacgct gaagaaatgg gtgctgatta ttattcaaag gatgcaaagt ctgctgttga
    18001 aatagcaaag ttaaattttt agcatataaa ttaaaataaa ggttttacta aataggatat
    18061 tttatatata atataaatat aagttttaga tacttatgtt taaaacaata ggaattaatc
    18121 tataataatg agattaatta atataaaaaa tatttatcat caacattaaa aagggctggg
    18181 aagtctggta taacttccat cagccctttt tagttaaaat catatgttaa aaagcccaat
    18241 taccattttt aaaaatctgt atttgctttc catcataagt ctcaccaata atatccatat
    18301 cagcagaacc aatcataaaa tctacatgtg ttaaactgtc atttacacca tgttcatcta
    18361 attcttcatc ttttaaattg tttccacctt ccagacaagt cttataagca gaaccaattg
    18421 caaagtggca agaagcattt tcatcatata gagtattata aaaaattata tccgtattgg
    18481 atattggtga gttgtatgga acaagagcaa cttctcctaa ataatgagag ccttcatccg
    18541 tttcaataag tttttctagt gtatctagtc ccttttcagc agagaaatct attattttac
    18601 catctttaaa tgttaagaag aaattgtcaa ttatgtttcc accataaaca agaggttttg
    18661 tactataaac agttccattt actttaaact tatgtggcat tccaaaaatc tcttcagttg
    18721 gaatattcgg attaaaactt attccattag ggtctttgga tgctccactt agccaaacat
    18781 gaccctttgg caagtctaga gtcaaatcag tttttgagga tttatatttt aaagttttaa
    18841 agttattttt atttagaaag tccattttag atttaagatt ttcattatgt tcttcccatg
    18901 cttttatagg gtcctgacca tctactcttg aacatttaaa tatagcatcc cacagttttt
    18961 ctattgcttt atcttctgat aaatttggaa acactttttt tgcccaatct tttgttggca
    19021 cagagactat actccattta catttatcag ttagagtgta agaacgccat tcttttaaag
    19081 cttttccaga tgccttctga aaatttgcaa ctctttcagg gtcaacatct tttaataaat
    19141 ctggattttg ggcatagact gataaaaatg ccccaccttc ttttgctata tctacatatt
    19201 gttctgctgt ccatttgggg aactcatgaa atgcttcatc tggagcatat aagtatttaa
    19261 taagagtact ttcttcatca gaccactcta tataaacatt tttagcacca catttatatg
    19321 cttctttaag tgcatttctt gcaaaatcag cacattctat tggtgacttt ataagtagaa
    19381 tttgacctgg ctgtatgtta actccaactt ttattgctaa ttcagcgtat ttttctaaat
    19441 ttctatttaa attcatattt atcctcctat atttttaata ttgaataact tataaagtat
    19501 tataacatta cttatgtttt taatattgaa caacttataa aatattataa ctttacttag
//
LOCUS       DAYATN010000063        19352 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.063, whole genome shotgun sequence.
ACCESSION   DAYATN010000063 DAYATN010000000
VERSION     DAYATN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.063"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            195..716
                     /locus_tag="ACGI8A_002157"
     CDS             195..716
                     /locus_tag="ACGI8A_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0180154.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            750..1526
                     /locus_tag="ACGI8A_002158"
     CDS             750..1526
                     /locus_tag="ACGI8A_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFH0180155.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDKI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1868..2821
                     /gene="gpr"
                     /locus_tag="ACGI8A_002159"
     CDS             1868..2821
                     /gene="gpr"
                     /locus_tag="ACGI8A_002159"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HFH0180156.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2839..3858
                     /gene="spoIIP"
                     /locus_tag="ACGI8A_002160"
     CDS             2839..3858
                     /gene="spoIIP"
                     /locus_tag="ACGI8A_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HFH0180157.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3889..4140
                     /locus_tag="ACGI8A_002161"
     CDS             3889..4140
                     /locus_tag="ACGI8A_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180158.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4422..6227
                     /gene="lepA"
                     /locus_tag="ACGI8A_002162"
     CDS             4422..6227
                     /gene="lepA"
                     /locus_tag="ACGI8A_002162"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFH0180159.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6376..7713
                     /locus_tag="ACGI8A_002163"
     CDS             6376..7713
                     /locus_tag="ACGI8A_002163"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFH0180160.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7946..9259
                     /locus_tag="ACGI8A_002164"
     CDS             7946..9259
                     /locus_tag="ACGI8A_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0180161.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9431..10606
                     /gene="hemW"
                     /locus_tag="ACGI8A_002165"
     CDS             9431..10606
                     /gene="hemW"
                     /locus_tag="ACGI8A_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFH0180162.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10709..11728
                     /gene="hrcA"
                     /locus_tag="ACGI8A_002166"
     CDS             10709..11728
                     /gene="hrcA"
                     /locus_tag="ACGI8A_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFH0180163.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11758..12378
                     /gene="grpE"
                     /locus_tag="ACGI8A_002167"
     CDS             11758..12378
                     /gene="grpE"
                     /locus_tag="ACGI8A_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFH0180164.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12470..14317
                     /gene="dnaK"
                     /locus_tag="ACGI8A_002168"
     CDS             12470..14317
                     /gene="dnaK"
                     /locus_tag="ACGI8A_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFH0180165.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14528..15682
                     /gene="dnaJ"
                     /locus_tag="ACGI8A_002169"
     CDS             14528..15682
                     /gene="dnaJ"
                     /locus_tag="ACGI8A_002169"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFH0180166.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16648..17583
                     /locus_tag="ACGI8A_002170"
     CDS             16648..17583
                     /locus_tag="ACGI8A_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFH0180167.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17619..18992
                     /locus_tag="ACGI8A_002171"
     CDS             17619..18992
                     /locus_tag="ACGI8A_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0180168.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19180..19338)
                     /locus_tag="ACGI8A_002172"
     CDS             complement(19180..19338)
                     /locus_tag="ACGI8A_002172"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0180169.1"
                     /translation="MSKTSTSMFLHRNTLVYRLEKINEILGFDLKNFENAMIFYLSVK
                     SYFLYKKI"
BASE COUNT         7674 a         2128 c         3525 g         6025 t
ORIGIN      
        1 attagtatgt atgatagatg catcaaacaa tataactaat atgttagaag gttatatgta
       61 attaatgtca gtgtttatat agaatagcat ttgtagcatt tattatagtt gtaattacta
      121 tttatatcat agacttgaat agtctaaaaa aatagactat aatattttta tagactaaag
      181 aaagggctga taagatggac atagcaaaac taattttaaa tcctgcaagg cttcgtattt
      241 tacaatatat tcgactttat ggcagtgttc gtacatctga tattgtaaag tatttaaatg
      301 atattcctcg tgcaacagtt tatcaccatg taaaaatttt agaagagaac aatatgattg
      361 aagtaattaa agaaaatcgt gtgcgtggta ctattgaaaa ggtttatgca ctaaaggaat
      421 atacaacatc tatggaagga gagaccttcg ttgcactttc tacagcattt tatgtaggtc
      481 tgatgcagga aatgaatgaa tactttagca gaaaagagca agaccataaa aaggataatg
      541 tatttttctc ttcagcatta ctatatatga gtgataatga atatgaaaat cttcttaaaa
      601 gtatagctga tttattgaaa ccatatatag aacaaaaacc taaatcaaat ctaaaactta
      661 gaaaactttc aattatatct tctccacctg tagctgataa tgataaaaca aaataacatg
      721 caacccattt ttataaaagg aggttatata tgaaatttca tgagtttgga aataaaaaca
      781 gaccacatat tatgttgatt catgggggag ggaatgcatg gtggaactat ttgcgacaag
      841 cagaagtttt gtctaacaaa tatcatgtga tacttcctac attggatggt catggagaag
      901 aatatgaaac aacatacatt tctacagagg atacagcaga taagttgata tcatatatag
      961 atgaaaattg cagtggacat cttttcgctt tgtgtggggt atcacttggt ggtcaaattg
     1021 tcatggaact tttatctcga aaacctaata ttactaaaaa agcaattatt gatggtagta
     1081 tttgctaccc aagacctctt atggcaaggt tttgtattgc tggtattcga ttttttggag
     1141 gacttttgtt tagtgagaag gcttgtcgct ttcaaattgc tatgatgcca aaattgttac
     1201 ctgaaaatat gcaatatcca gataaaataa aagcgtatta tatgacagat atgccacatg
     1261 taagaaaaga aacattctat aatatgtatc gtacttacat gatgaactat acacttaaag
     1321 agaatgttaa aaaaaccaca gcacaagtaa tgtactggta tggagaaaaa gaaatgaagt
     1381 gtgtcaaaaa atctgcaaaa ctgtttcagt cttatgtgcc ctcttgtgag atttatgaag
     1441 ctaagagcta taatcatggg tatttatcac tttatcttcc aaatgaatgg ttggaaattg
     1501 cagaaccatt tttccaaaag gaataaaggt gttaggtgat tattgtataa ttattgctgt
     1561 ttttttatat agtaaataaa ttaggtataa atataaaaaa ttattttagt taatatatta
     1621 gtgaaagctt aataggaaaa ttataaatag attaaaggta gcacctatat aaatagttgc
     1681 tacctttttc tgtttgtaaa taaatgatag ataagaaggt ttactgacaa atcactgact
     1741 aaacccaatt ttcttacatt gaaagcctta attttacttg ttttaaataa atttacaatt
     1801 tcttgttata aaatactaaa ttttgttaac aattataatt agcatttaat ttatactgga
     1861 ggtttatatg attagtgtaa gaacagattt agctttagaa gctagtgaaa tgtgtgaaaa
     1921 gtctcaagaa ggaagtagta ttcctggtgt aaaaatagaa actaaagaat tagaaaattg
     1981 cattgtaact aaagttgaag taatagacag gcaaggcgca gagataatga ataaagatat
     2041 aggaaagtat ataactctgg aaagtaatct tatgaagttt gatgatgatg agtcaagaga
     2101 agaaatgata agttatttaa aagaggagtt agtagatata tttggccaag acaaaaataa
     2161 aaaaaccctg gtaattggtc ttggaaatag aaatattact tctgatgcgt taggaccaaa
     2221 gagtgtatca aaaacacttg taactagaca tctttttaaa aattataata aagactatga
     2281 tgatgatttc actgaggtat cagctttaag cccaggagtt atgggtgtta ctggaataga
     2341 aactagtgaa atagtaaaat cattggttga gaaggttaag ccagatagag tagtagcaat
     2401 agatgcattg gcttcaagaa aaatggaaag agtaaactct actatacaga tatcaacagc
     2461 aggaatatca cctggtggag gtgttggcaa tactagaaaa tctctaacta aagaaacttt
     2521 aggcgtggat gttattgcaa taggcgttcc aacggtggtt gatgcagcaa ctcttacaat
     2581 agatgtctta gatatggcaa tagacaattt aatagctcaa tcagaagaaa cagaaagttt
     2641 ttatgaaatg ttgaaaaaac ttaaagaaga agaaaaatat catctaataa aagattcgtt
     2701 agacccatat gacaagaact taatagtaac accaaaggat atagatgata caatcgaaaa
     2761 tctatctata ataataagtg aaggcttaaa taggtcttta catccaggta gattggtata
     2821 aaataaggag gagcaattat gtttaaaaaa tgcattaaag tagtaacctt gacttttata
     2881 ttagtatgca ttttaccagg aaaatcatta gctttgaatc aagatgattt tttaaagttt
     2941 ttagtaaatt catcttatcc agaagctaaa gtcgaaggaa atgatacaga aaataaaaag
     3001 aataataaaa ataaagaaac aagtaaggaa aataaggaag aaagtaaaga aggaaacact
     3061 aaatctaaag atgcaagtaa agttgataat aaaaaagaga gtgaaaaaga atatataaaa
     3121 ctttatgttg gtaaagaaaa tgttccagat attgagtcaa aaaattcaga cacaactgaa
     3181 actaacacaa catctagttc agattataag gatgatttaa gagtaaccaa agaaaatcca
     3241 agaattttga tatatcatac tcatggatgt gagacttatt caaattcacc tgatggaaat
     3301 tatcattcaa gagataagaa aaattcagta atggaagtag gaagtgcatt aactagtgct
     3361 ttagatagca aaggatgggg tgtagtccat actactaaat accatgacta tccatcttat
     3421 aataactctt atgcaagtag cttaaaaaca atacaaagta tattacctaa atataactca
     3481 gtggatatag cgatagactt acatagagat gctagggatt taactaatcc agccactaag
     3541 gaaaaagacc atttaaaata tacaactatg ataaatggag agagagtatc aaagttcttc
     3601 tttgttgtag gaggtaaaaa tacaaataga aaacaactta gagctttagc agaagatata
     3661 actgcttttg ctgagaaaaa gtatcctggt ctagtatctc caatagtaga aaaagactat
     3721 gctagattca atcagtttgc agttaagaat catatgttag tagaaatagg gaataacgca
     3781 acaagtgtag aagaatctaa ggctactaca aagtatttag ccgagatttt ggatgaatat
     3841 tttaaacaaa aaaattagag ctttaagtta taattaggag catgctaaat gagccgatta
     3901 gaaaaaaata tagaaagtaa aagtaaacta agaaaataca agtatattat gagaatctta
     3961 tttttgatta ctatgattat atgtacatca gtttgtgtat ttatagtgga cagtaatgca
     4021 aaaagtatgc taggaatgaa acaatatgat tttccaagta ttaaatttga tataacaaaa
     4081 tttgattttg acataccaaa ttttgatata catgaaatca taaaaaaatt taataagtag
     4141 taataagtga ataaggttta ttgattaaaa taagccttat tttagttata tatttttaat
     4201 gtataaaata aaaattcatt aactgtattt tataggattt actttaagtt tatgatatta
     4261 tataatattt agtgttagac ttgataatcc acatataatg tggataattt tgtttagtgc
     4321 caattagttt atattcaaaa attttaaatt tggtatataa agagttttta gataatgtaa
     4381 aataataact ataagaataa tcaagaggag ggaattttaa ggtggacaat aaacaaagta
     4441 gaactagaaa ttttagtata attgcacata tagaccatgg aaagtctacc ttagctgata
     4501 gattaataca acaaactggg cttgttagtg aaagagacat gaaaagtcaa ttacttgaca
     4561 atatggacct tgaaagagaa agaggaatca ctataaagct tcaaaacata agattaatgt
     4621 ataaagcgaa ggatggaaat gaatattatt taaatcttat agatacacct ggacatgtag
     4681 actttaacta tgaggtatca agaagtttag ctgcatgtga aggagcacta ttagtagttg
     4741 atgcagctca aggtgttgag gcacaaactt tagccaatgt ttatcttgct atagaccaag
     4801 atttagaaat attaccaata ataaataaaa tagatttacc aagtgcaaga ccagaggaag
     4861 taaaaaatga aatagaagat ttaatagggc ttgattcaag tgaagctcct cttatttcag
     4921 ctaaaactgg tttaaacata gaagatgttt tagaagatat tgtaaaaaat gttcctccac
     4981 ctaaaggaga taatgaagct ccattaaagg cattaatatt cgattcttac tatgatgctt
     5041 acaaaggtgt tgtagcttat gttagagtat ttgaaggaac tgttaaaaag ggtatgacaa
     5101 taaaaatgat gaataccaat aaaaagtttg aggtaactga agttggtgtt atggctccag
     5161 gtcaaactga acttagtgaa ttatcagcag gtgatgttgg ttatattgct gctagtataa
     5221 aagatataag aagttgtcgt gttggtgata caataacaga ttcaaataat cctacagaag
     5281 aacctttacc aggatacaaa aaagctactc caatggtata ttgtggtata tatccaggag
     5341 aaggagaaaa gtatgagaat gttagagatg ctcttgaaaa gcttcaagta aatgatgctg
     5401 cacttgaata tgaagctgag acttcagcag cactaggatt tggttttaga tgtggtttct
     5461 taggtctttt acatatggag ataatgcaag aaagacttga gagagaattt aaccttgata
     5521 taattactac agctccatct gttatatata gagttacaaa aatggatgga gaggttgtaa
     5581 tgatacaaaa tcctgcaaac ttaccagaac catctgagat aaagatgata gaagagccta
     5641 tagtaaaagg agatataata gttcctaaag actatgtggg tgttgttatg gagctttgcc
     5701 aggaaagacg tggaaatatg ttaaatatgg aatatataga tgatagaaga gtaatgcttc
     5761 actatgacct tcctctcaat gaagttgttt acgatttctt tgatgcttta aaatcaagaa
     5821 ctagaggtta tggttctctt gattatgagg ttaaaggtta tgtagcatct acacttgtaa
     5881 aacttgatat attaatcaat aaagagcaag tggatgcact tagttttatt gtccatgaga
     5941 cacgtgcttt ccctagagga aaggcaatgt gtgagaaatt aaaaggagaa attccaagac
     6001 atcagtttgc tataccaata caagctgctg ttggtaataa ggttattgca agagaaacaa
     6061 taagcgctct aagaaaagat gttcttgcta aatgttatgg tggagatata tctcgtaaga
     6121 agaaacttct tgaaaaacaa aaagaaggta agaagcgtat gagacaaatt ggttctgttg
     6181 aagttcctca aaaagcattt atgtctgtgc ttaaattaga tgagtaaatc cttataaatt
     6241 tagataaaga tatttaaaag ctagagtgat tttagaatta tttctaaatt agtctagctt
     6301 ttttatggta tattttaagt aaactcatag agaatataag taagaatatt aaagaagaat
     6361 aaaaaggtga tatttatgat taatatagaa attcctaaaa aagtagacta tataattaaa
     6421 gaattagaaa agaatggtta tgaagcttat atagttggag gatgtgttag agattgttta
     6481 ttagaaagag ttccaaacga ttgggatata acaactagtg ctagaccaga agtggtagta
     6541 gaattatttg aaaaaacaat tccaactggt atacaacatg ggactgtaac agttatgatt
     6601 gaacatgaac catttgaagt gacgacttat agaattgatg gaaattatag tgatggtaga
     6661 catccagatt caatagaatt tacaaataac atagttaagg acttgtcaag aagagatttt
     6721 actatcaatt caattgcata caattcaaag acaggattag tcgacccatt taatggctat
     6781 gaagatatac aaaataagta tatacgttgt gttggaaatc cagtagatag atttgaagaa
     6841 gatgcactta gaatgcttag agcagttaga ttttcagcac aactcaattt taaaatagct
     6901 gaaggtacta agcaaagtat tcataaaaaa gcagatttga taaaaaatgt ttctattgaa
     6961 agaatccaga cagagtttaa taaaatatta gtatctgatt catctaaatt aaacttatta
     7021 aaatcaacag gtttgttgaa atttataatt cctgaaatat gtgaattaga agatgttact
     7081 caacacaatc catatcacat ctatgatgta caaaaacata ctttaatagc tacagaagta
     7141 attgaagatg aactgtattt aaaacttaca atgttgtttc atgatttagg taaaaaagta
     7201 actaaaacaa ctgataaaaa tggtgttgac catttttata ctcatagtag agaatctgta
     7261 aagattgcaa agaagatttt gaaaagactt aagtatgata attatactat taataaagtt
     7321 ttaatattaa tacaatatca tgattataga atagaaccta aaaggaagat aattaaaaag
     7381 ttattaaata aacttgaaga tgttgaatta tttgaagatt taataaaagt taattgggct
     7441 gatacacttg caaaaaatcc aaagtatgca aaacaaaaga tattaaatct tattgagtgt
     7501 gaaaaagaat ttaaacatat aataaatcaa aaggagtgct ttaatcttaa agatttagct
     7561 attaatggaa aagatttgat atctataggt ataaaaccag gtaaagatat aggatatatt
     7621 ttaaataaga tgcttgaaat agtcataaat aatccagagt taaatgaaaa agaaatacta
     7681 aaagaaaaag cattaaatat atatacattt tagattcaaa tataaaaaat ttaattactt
     7741 taaaaaacat agaataaaat acttatttag ataaaataaa taatttttag agaatatttt
     7801 agaagttacg attgtatgat ttttaaatgc ataggcattt actctaggac tatagactaa
     7861 tataaaaaaa aactttggga atactaaaac cataaattta taaaattaat attttatata
     7921 ctgtaaaaca gtgaggaggg acagtatgca tactcaacta caaaagacta ttggtctttc
     7981 agcagctctt tctactgttg taggtatggt tataggttcg ggagtttttt ttaagccaca
     8041 agcaatatac actacaacta atggagctcc aggtctaggt attatagcgt ggcttttagg
     8101 tggttttata acaataactg ctgggcttac agctacagaa atttcagctg ctataccaaa
     8161 aactggtggt atgatgattt atatcgaaga aatatatgga gaaaagttag gttttttaac
     8221 tggatggatg caaactgtat tatttttccc aggtacatca gcggcattag gagtaatatt
     8281 tgctcaacaa gcatctgaac ttttaggaat gagcccaaat aatatggcaa atgtacttcc
     8341 tatagcaatt ggagttatac tatttttagc gttattaaat ataataggtt catccttagg
     8401 aggaaaagtt cagacagtag ccactattgg aaagatgata cctttaattt taataatagt
     8461 ctttggattt attaaaggtc aaagtagtga agtattaaat ccatttgtag gagatggtgt
     8521 aaatgcatca aatgcacttg gacaagtatt aatagctact ttgtttgcat atgatggttg
     8581 gataaatgtt ggagctatat caggagaaat gaagtctcca gagaaagatt taccaagagc
     8641 aatagttggt ggtttatcat tagttatggc agtttatatt ataataaata tagcttatct
     8701 ttgggtagta ccagcaagtg aattagcaac agttacttcc ccagctacat tagtagcaac
     8761 aagattgttt ggaaatatag gtggaaaagt aataactgtg ggtatattaa tatcagtatt
     8821 tgggacattg aatggatatt tattaacagg ttcaaggata ccttatacat tagctgaaat
     8881 gggaacttta ccagcatcta aaactctttt aaaagtaaat tctggaggtt ctcctgtaaa
     8941 ttcaatacta cttataactg tgttagcctg tgtatatgca ttatctggtc aatttaatct
     9001 tttgacagac ttaacaatat tttcaatatg ggtattttat gtattgactt ttataggtgt
     9061 tatgaggctt agaagacaaa agccagattt acacagacca tataaagtcc ctttatatcc
     9121 aataatacca attatagcta tattaggtgg actgtttgta ataataaatc aaatattgac
     9181 ttcaacagtc atatctttag gcggaatatt tataacacta ttaggtttac cagtttatta
     9241 ttatatgaaa aaaagataac tacttaattt aagttagata aatttgggta aaggtgaaaa
     9301 ttaactttta tccaaatttt ttatactgtt atacaatata agtattatat attgcttgat
     9361 gtgctatttt gtttgattta taatttagta attgatgttt taagataatt taagaaatga
     9421 ggagtgaaag atgctaggac tttatgtaca tattccattt tgtgtgaaaa aatgtaagta
     9481 ttgtgatttt aattcttata aaatggacat agattcaaaa aaaagatata tagaagactt
     9541 aaaaatagaa atggaattat atagtaataa attatataaa gataataaat ataaaaataa
     9601 agaatgctgt agtctaaata aaaatgacaa aataacgagt atatttgtag ggggaggtac
     9661 acctagtata ctaacctctg atgaaataag agaagtattt ataagtataa aagaaatgtt
     9721 tgatatagat gagaatgcag aaataactat agagtgtaat cctgggacat taacattaga
     9781 aaaattaaaa actatgaaag agattggcat aaatagactt agtatagggc tacaagctat
     9841 tcaagaaaag catttgaact ttataggaag aatacatact tatgaagaat ttgagaaaaa
     9901 ctataaagat gcattaagtg taggttttaa aaatattaat atagatttaa tgtacagtct
     9961 tccaaatcaa actctatgtg attggaagga aactcttgaa aaagttgtac acttaaatcc
    10021 aacacatata tcagcttatt cccttatact agaagaagga actgaactat acaatatgta
    10081 tgaaagtaat aaatttgaac ttatagatga aaatgtagat atagaaatgt atgagtatac
    10141 aataaactat ttaaagtcaa aaggatataa tcaatatgaa atatcaaact actcaaaaga
    10201 aggctataat tgtgagcata atattttata ttgggaatgt gaacattata tagggattgg
    10261 agcaggagct tctggctata ttaatgagaa cagatacaat aatgtagaat ctttagaaga
    10321 ttatcattta agtttggtaa aaagagaaaa accaatacaa gaaaatgaaa ttctttctga
    10381 gaaagatatg atagaagaaa agatatttat ggggcttaga atgaataaag gcataaaatt
    10441 tgaagatttt aaaaaaaaat ttggaataga ttttagagag aagtataata agcaaataga
    10501 aatgctatta gctagaaagc ttataaatca gagctttgaa ggtatacaat taactcaaaa
    10561 aggaagagaa atatctaata gtgtatttat agaatttatg gaataatttt taaggtgttg
    10621 acaaaataaa tttataatgg tatataatat atataatctt tttagcactc gaacttagtg
    10681 agtgctaaca gctaggaggt ataataatat ggaattaaat gaaagaaaat taaatatcct
    10741 caaagctata gttaaagatt atatagaaac agctgaagct ataggttcta gaacaatatc
    10801 taaaagacat gatttaggtg ttagtgctgc taccataaga aatgaaatgg ctgaccttga
    10861 agaattaggg tatttaattc agcctcatac ttctgcgggt agagttccat ctgaaaaagg
    10921 ctataagtta tatgtaaact ctcttatgag taaaagcgaa ttggatgata atgataagat
    10981 tcttattgaa caatgtatga atcataatat aaatcatata aaagaattga ttcatgaaac
    11041 ttctaagcta ttatcgcaac taacaaacta tacaactgta gcagttacta agagtttaat
    11101 aaatcaaagt gttataaagc acatacagtt ggttgctatg aatgacaata atattttact
    11161 tatagttgta acagataaag gggacttaaa aaaagctaac cttacaacta atgtatattt
    11221 agatcaatct aagttaaatt tgatttctga taatcttaca aggaagctct taggtaaaag
    11281 tataacagat ttggatgata atctaattgc ttttattaaa tacgaaataa gtgagtattc
    11341 tgggcttata gatgagcttt taaatgcttt gaactcaaat atgaaagaag aagatttttc
    11401 tctatcatta aatggagcta ctaacatatt tagttatcca gaatttaatg atgtattaaa
    11461 agcaaagtca tttttaaata tgttggaaaa aaaggaaaca atagctgaca taattaaatc
    11521 aaagggaata caaaaagaca atctaaatat aataattggt agtgataatg attgtgaact
    11581 ggcacaggat tgtagtatag ttacagcaac ttataatgta gatagagact tggttgggcg
    11641 aataagcttt ataggcccta ccagaatgga ttatgctaga atttactcta taataaatta
    11701 tatgagttta ttaattaata gaaagtaaag aaattttaaa ttgcgaggtg taaagtcttg
    11761 gataaaaaaa atgagcaaca ggaagtaaga gaagaaaatg atacttctat taaccaagaa
    11821 tcggaaactc aagtagagtt agaagaggaa gttgttaatg aagaatgtga aacatcaagt
    11881 gaaaaaactg atgaaaaaga agttgatgat gaaaatgtaa cagatataaa ttctaagtta
    11941 gcagaaaaaa aattgcagga tgagttggat gaattaaatg ataaatatca aagacttcaa
    12001 gccgaatatg caaactacag aagaagaact caacaagaga aagagacaat aggcgtattt
    12061 gcaaatgaaa aaataataac tgaattgata ccagttatag atagtatgga aagagcctta
    12121 gatgcatgtg aagataagga agatactatg tataagggta taagccttgt acataaacaa
    12181 ttaatagaca ctttagtaaa atttggagta gaagaaatag aagcagaatc taaggaattt
    12241 gaccctaatc tacatctagc agttatgcaa gaaagtgtag atggagttga agcaaatcaa
    12301 atagtaatgg tacttcaaaa aggatataag ctaggaacta aagttgtaag accttcaatg
    12361 gttaaagttt cttgctaagt ataaattaaa ggttaaatta tttaacctaa tataaaatat
    12421 taaaataagt aaaacttaat tatataatta ctcgaggagg attttagtca tgggaaaaat
    12481 aataggaata gatttaggaa caacaaattc atgtgttgca gtgttagaag gtggagaagc
    12541 acaaataata gcaaatagtg aaggtatgag aactactcca tcagtagtag catttacaaa
    12601 agatggtgaa agaatagttg gagaacctgc aaaaagacaa gcagttacaa atgcagataa
    12661 aacaataact tctataaaaa ctcatatggg aactgattat aaagtaaata tagatggaaa
    12721 atcatatact ccacaagaaa tatcagcaat aatattacaa aaattaaaat cagatgcaga
    12781 aagttactta ggacaaacag taacagaagc agttataaca gtaccagctt actttacaga
    12841 tgctcaaaga caagcaacta aagatgctgg tagaatagca gggcttgatg ttaagagaat
    12901 aataaatgag ccaacagcag cagctcttgc ttatggtatg gataaattag accaagaaaa
    12961 gaaaatatta gtatttgact taggtggagg aacttttgac gtatctatac ttgaaatagg
    13021 agatggaact tttgaagttt tagctacagc tggtaacaat agattaggtg gagatgactt
    13081 tgaccaaata gtaatagatt atttagcaga ggaatttaaa aaagctgaag gtgtagattt
    13141 aagaaatgat aaaatggctc ttcaaagatt aaaagaagca gcagaaaagg ctaagaaaga
    13201 attatcatca acaatgagtt caaacataaa cttacctttc ataactgcta cagcagaagg
    13261 accaaaacac ttaaatatag atttatcaag agctaaattt gaagaattaa ctagaggttt
    13321 agttgaaaaa actatggaac caactaagac agctttacaa gatgcaggac tttctacagg
    13381 tgatatagat gatgtattat tagtaggtgg ttctacaaga ataccagcag tacaagaagc
    13441 tgttaagaag tttataggaa aagaacctca taaaggtata aacccagatg agtgtgttgc
    13501 agcaggtgct tctatacaag ctggagtttt agcaggggat gttaaagact tattattact
    13561 agatgttact ccattatctc ttggtataga aactatgggt aatgtaatga ctaagataat
    13621 agaaagaaat actacaatac caactaagaa gtctcaaata ttctcaactg ctgctgataa
    13681 ccaaacagct gttgatatac atgttcttca aggtgaaaga agtatggctt atgacaatac
    13741 aactttagga agattccaat taacagatat accaccagca caaagaggaa tacctcaaat
    13801 agaagttact tttgatatag atgcaaatgg tatagttaat gtttcagcta aagatttagg
    13861 tacaggaaaa gagcaaaaaa taactataac atcaaacact aatttatcag aagcagaaat
    13921 agagcaaaag ataaaagaag cagaaatgaa tgcagaagct gacaaacaaa agaaagaaaa
    13981 aatagaagca ttcaatcaag ctgaatctac tatatatcaa actgaaaaaa ctttaaatga
    14041 acttggagat aaaataagtt caggtgaaaa agaggatata gaaaaagcta tagcagactt
    14101 aaaagctgta aaagataatc aagatgctac agcagaagaa cttaagaaag ctacagatga
    14161 agtaatgact aagttccaaa aagtatctca agaaatgtac caaaaagcag ctcaagaaca
    14221 acaagcggca caaggtgcag aacaagcaca agacaatgga cctaaagatg acaatgtagt
    14281 agatgctgac tttaaagaag tagatgaaga taaataagct atataatatg taacactaaa
    14341 tgataattgg aacatatatg atttgtgaaa atcatataga tgttaaagat atatggacaa
    14401 tatacatcat aaagaatata atatatcata gattatataa cttaaagtag aatataagaa
    14461 ttaatgatag cttgtagttt attattctac aagctatcgt tttgtaaaat gaaggtggtg
    14521 tcaatttttg agtacaaaaa gggattatta tgaagtctta ggtatcagta aaggtgcaga
    14581 agctcaagag ataaaaaaag cttatagaaa attagcaatg aagtaccatc cagatagaaa
    14641 tcctggagat aaagaggcgg aagaaaaatt taaagagata aatgaagctt atgaagttct
    14701 atctgatgat acaaagagaa aaacttatga ccagtttgga catgatgggc taaatggtca
    14761 aggcggtttt ggtggtcaag gaggatttgg cggtcaaggc tttggtggtt ttgaagatat
    14821 gttcggagat atatttggtg acatgtttgg aggaagcttc ggaggtggaa gagcaagaag
    14881 aagagggcct caacgtggtg cagatataag acagagtgtt actatatctt ttgaagaagc
    14941 tgcatttggt aaaaagatgt ctataaaagt aaatagaagt gaagaatgtg aagaatgtaa
    15001 tggaactgga gcaaaaccag gaacatcaaa gaaaacttgt tcaacttgta atggaactgg
    15061 tcaagttaga actgttcaaa gaactccatt tggtaatata gcaagttcaa gaccatgtag
    15121 tgcttgtaat ggaactggag aagtgattga atctccatgt agtaaatgtc atggaactgg
    15181 aaatacaaga aaagttaaga ctatagaagt agatatacct gctggtatag atgatggtca
    15241 aatgataaaa ctttctggtc aaggtgaagt aggagaaaaa ggtgctccaa gaggagactt
    15301 atatattgta gttaatgtta agtcacatcc actatttact agagatggaa atgatattta
    15361 ttttgaaatg ccaattacat ttgttcaagc aactttaggt gatgagatag aagtacctac
    15421 tttggatgga aaagttaaat atagtgtgcc agaggggaca caaactggaa ctgtatttag
    15481 acttaaagag aaaggaatcc ctagaattag aggtaattca agaggagacc aatatgttaa
    15541 ggttgtagtt gaaattccta aaaaattaaa cgataagcaa aaagaactat tgagagaatt
    15601 tgcgaaggag tgtgggtcga acgtccacga aaagaagaaa accttcggac aaaagataga
    15661 agatatgttt aagaaaaagt agttaaagta gccaatatat aatcatattt ttaaaatctt
    15721 agttatttat aagattgtca aaggagatat tatgattttt atattggcta tttttgtata
    15781 aaaaataaat gtgcataaaa taaaactgaa tataaataat tttctgaaaa atacttgtcc
    15841 taaaatatat aatatgctat aatataatca aataaaagaa aggggtgatt ccagtgggaa
    15901 cggatgtggc actgccaaat gatgaagaaa actttattgt aatatacaaa ataaaattag
    15961 atacttttag tgctagatta agtactggaa aaactcatga gctgggataa ttattgtaat
    16021 tacctagact caaactttta gtagtaaaat agtaatatta tatagacaag gtatctaatt
    16081 tttaaatctt ttgtatgaat taaaattttt ataaagggta tatctgatta gaatgtaatt
    16141 agggaaacct agttgtgaat tacttttagt atatacatgt gtaaagaaat atgtatatgg
    16201 tattggttat tgtgctagat gtgtatgtaa aggaatatgt gtctagttgg gtatatgtaa
    16261 agaaatatac ttataatcta tgaaaaattt ttagtataaa ttgctaaaac attgagattg
    16321 ttttttatag aaagttagtt tataccaact aaaaatgtgc ttaagatttg tttgatgttt
    16381 aattataaat aaggttgtag ataaaaaaat aagtaaagac aaaggtttaa aatacttgct
    16441 ttaaatatag tttgttttga acacttgcca aattaaaata attaataaaa tagacatctt
    16501 ctcatagtaa aatatactgt gagaggatgt ttttgctttg ttcataaact tttatgaaaa
    16561 atttgcatag tgtcacattt taatatacta tatatataat aaaatataga taaaattaaa
    16621 tgattcaaaa gagaaaggtg aaaagagatg tattatatag gtgtagacat aggaggaaca
    16681 ggtatacaag caggagtagt tgataactat gggaaaataa tttttaggtc agagtgtaaa
    16741 acagtaattg aaaaaggctt tgaaggaata cttaatgaca taaaaataat gatttataag
    16801 ttgttggaag ataataaact tactatgagt gacataaaat ctattggatt tggtgttcca
    16861 ggttttataa ataaagaagg attagtaact tgtgtaaatt taaaatggaa taaaaaagct
    16921 tttaataaag agttaaaaag aagatttcca gatgttgaaa ttcatggaga aaatgatgca
    16981 acagtagcag cacttggaga agcaaagttt ggaagtatga aaggtgctaa tgttggagta
    17041 ttatatactt taggaactgg agtaggtgga ggaatagtaa ttaatcaaaa agtcttttct
    17101 ggagcacatg gattgggttc agaaataggt catcaaatta taggagaaaa ctattttaat
    17161 tgtaattgtg gaaataatgg ttgtgtagaa actttttgtt cagcaactgc aattataaag
    17221 tattctcaaa aactgataga agaaggagaa aagagtagga ttttagattt ggcagaggga
    17281 aatttagaaa atgtaaatgc aaagatggtg tttgatgcat atagagaaaa tgatttagtg
    17341 gcaattaaag taataaatag atttaaagag tatttagcaa aaacttttgc aaacacaata
    17401 aattctttag acccagaaat tatatctata ggaggaggca tttctaaatc tagcgatata
    17461 atacttgatg gaatagaaga tttggtaaga aaatttgtat tatacaaaac agaagatata
    17521 gctacaatta catgtgcaac tttaggttca gacgcaggaa ttataggagc agctttttta
    17581 taatgtaaat gaactgctta ttggaggcgt gatgatatat gggtaaaaca aatgctagaa
    17641 gaacgctttt tttgatagca ataggttcag gaactatgct gaatcctctt aattcttcta
    17701 tgatttcact tgctttacat agtatacaaa atgattttca tattagtttt acaactgtat
    17761 cttggattgt ttcagcattt tacctagcta gtgctattgc acaaccagtt tcaggtaaga
    17821 taggagattt aataggtaga aaggtcttat ttttgagtgg tttgatgctt gtaattttat
    17881 cagcatccat agtaccactt gttcaatcat tctttatatt gatttttata agagtaattc
    17941 aagcaattgg aacaagtacc ctatatccat ctggagttgc tcttatacaa aataatataa
    18001 aagaaagaca atcttctgca ttagcagttc taaccatttt tgcttcaact acagcaggat
    18061 taggtccgac acttggagga ttattgttgg acttaggagg atggcatgct atatttcttg
    18121 taaatatacc tgttgttttg gtttctatat gcttagggta ttttttattc ccaaaagaag
    18181 taaaagaaaa gaaatctata aaagaaactt taaaaaatgt tttgtcaaga ttagatatta
    18241 ttggcatact tctattttca attgcaatga tttttatatt actatttttg ctatctatga
    18301 aggagtcttt taacttggaa caacttgtat ttggtataat actgatgtgt ctttttattt
    18361 ggcatgaatt gagaacaaaa ttgccattta tagatattaa gctttttgta agtaatccta
    18421 aattgtcaaa agtttacttg caatttatta tattaaattt ctttaattat attttgtttt
    18481 ttggtcttcc tagttatttt caagatgcct tacattatag tgcaaagtcg actggattat
    18541 ttatgctatt tatgtctggt ataggtatat ttatatctcc attaactgga aaatggatag
    18601 ataaatctgg aactagattt cctcttgtta catcatcaat ctttatgttt attagtgcag
    18661 tacttctttc agtagttttt atccatatac ctgtaatagg aaagggtatt atattatcat
    18721 tagcaggtat tagttatgga gttggtaatg tagccctgca atcctctatg tttgaagaaa
    18781 gtcctcgtga cagtataggg acagcttctg gtctgtttca aactagtcgt tatttaggtt
    18841 ctattttagc aactgtttta cttggaatgg tatttgaagt aaatataaca tcagaacaat
    18901 ttcagatatt aggttatgtt atggtggtct taggtacaat aagtttttta ttaaatttta
    18961 tattaaagaa agaattaaga aaagtagaat agtggattta tggatttaat aagtctaata
    19021 actgataagt ttaaaaatat tgtttgtaaa acataactgg tttgatattt attaattcta
    19081 agttttgaag catagagtac tcgataagta tacaatattt tgaattaaaa ttaagcagaa
    19141 atttgaacta gtctttcaaa tctctgctta atttagtagt taaatttttt tatagagaaa
    19201 ataagatttg acacttagat aaaaaatcat tgcattttca aaattcttta aatcaaaccc
    19261 aagtatctca ttaatctttt ccaatctata tactagcgtg tttctatgta aaaacatact
    19321 agtagatgtc ttagaaatat ttaaatcatt at
//
LOCUS       DAYATN010000064        19336 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.064, whole genome shotgun sequence.
ACCESSION   DAYATN010000064 DAYATN010000000
VERSION     DAYATN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.064"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(352..1551)
                     /gene="coaBC"
                     /locus_tag="ACGI8A_002173"
     CDS             complement(352..1551)
                     /gene="coaBC"
                     /locus_tag="ACGI8A_002173"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFH0180170.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(1556..1822)
                     /gene="rpoZ"
                     /locus_tag="ACGI8A_002174"
     CDS             complement(1556..1822)
                     /gene="rpoZ"
                     /locus_tag="ACGI8A_002174"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFH0180171.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(1825..2442)
                     /gene="gmk"
                     /locus_tag="ACGI8A_002175"
     CDS             complement(1825..2442)
                     /gene="gmk"
                     /locus_tag="ACGI8A_002175"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFH0180172.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(2472..3353)
                     /locus_tag="ACGI8A_002176"
     CDS             complement(2472..3353)
                     /locus_tag="ACGI8A_002176"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFH0180173.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(3357..4187)
                     /gene="dapF"
                     /locus_tag="ACGI8A_002177"
     CDS             complement(3357..4187)
                     /gene="dapF"
                     /locus_tag="ACGI8A_002177"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFH0180174.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(4474..5508)
                     /gene="ltaE"
                     /locus_tag="ACGI8A_002178"
     CDS             complement(4474..5508)
                     /gene="ltaE"
                     /locus_tag="ACGI8A_002178"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFH0180175.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            5804..7579
                     /locus_tag="ACGI8A_002179"
     CDS             5804..7579
                     /locus_tag="ACGI8A_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HFH0180176.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(7794..9446)
                     /gene="cplR"
                     /locus_tag="ACGI8A_002180"
     CDS             complement(7794..9446)
                     /gene="cplR"
                     /locus_tag="ACGI8A_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Contributes to intrinsic pleuromutilin, lincosamide
                     and streptogramin A resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein CplR"
                     /protein_id="HFH0180177.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(9745..10956)
                     /locus_tag="ACGI8A_002181"
     CDS             complement(9745..10956)
                     /locus_tag="ACGI8A_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFH0180178.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(11240..11773)
                     /gene="pyrR"
                     /locus_tag="ACGI8A_002182"
     CDS             complement(11240..11773)
                     /gene="pyrR"
                     /locus_tag="ACGI8A_002182"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFH0180179.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(12133..13080)
                     /locus_tag="ACGI8A_002183"
     CDS             complement(12133..13080)
                     /locus_tag="ACGI8A_002183"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFH0180180.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(13049..13495)
                     /gene="lspA"
                     /locus_tag="ACGI8A_002184"
     CDS             complement(13049..13495)
                     /gene="lspA"
                     /locus_tag="ACGI8A_002184"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFH0180181.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLVDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(13805..14584)
                     /locus_tag="ACGI8A_002185"
     CDS             complement(13805..14584)
                     /locus_tag="ACGI8A_002185"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0180182.1"
                     /translation="MKKIICGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(14639..15121)
                     /locus_tag="ACGI8A_002186"
     CDS             complement(14639..15121)
                     /locus_tag="ACGI8A_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFH0180183.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(15387..16808)
                     /locus_tag="ACGI8A_002187"
     CDS             complement(15387..16808)
                     /locus_tag="ACGI8A_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HFH0180184.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(16954..17133)
                     /locus_tag="ACGI8A_002188"
                     /pseudo
     CDS             complement(16954..17133)
                     /locus_tag="ACGI8A_002188"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
     gene            complement(17146..17724)
                     /locus_tag="ACGI8A_002189"
     CDS             complement(17146..17724)
                     /locus_tag="ACGI8A_002189"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0180185.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGI"
     gene            complement(17729..18931)
                     /locus_tag="ACGI8A_002190"
     CDS             complement(17729..18931)
                     /locus_tag="ACGI8A_002190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0180186.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         6357 a         3412 c         1958 g         7609 t
ORIGIN      
        1 acaggaattt gatatgtaaa taaattatct gtatatatag tattacttct aactattacc
       61 tttgcatatt ttttcattat aaaacctcta cttaaattta atatattgtt catattttaa
      121 tatactttac aagatattaa aatctcagaa tttataagca atttcaagtt attaatatta
      181 actttatttt aataattaac tattaattat attttatact ttatgacaaa ataaatgctg
      241 aaaactacaa tttaactgac atttgcgagt ttttactcgc taaaattaaa ttgcagcttt
      301 cagccacttt agctgtgata aaatacacaa atatcatatt taaaaaaggt cttatttctc
      361 aagcaacgct ttaactttat ctaaaataac attggctact tcttcttttt tcatcttagg
      421 atactcagta atatttccct ctacatctat tatttttaca atattagtat cagctctaaa
      481 accagcacct tctttagtta aatcatttgc cacaataaaa tctaaatttt tcttttttat
      541 ttttcctttg gcatgttcta taaggtcatt agtttcagct gcaaatccaa ttagtatctt
      601 atcttctttt atttttccta tttcataagc aatatcataa tttctactta gttctattga
      661 taaatcatca ttcttctttt taattttttt gtcagaataa gtttttggtt tataatctgc
      721 aactgctgca ctttgaatta ctattttatt ttcacttaaa tttgatacta ctgcttcata
      781 caaatcttgt gctgactcta tttgaacaaa ttttttaaga ttttgtggaa tagaaagatt
      841 tgtaggacct gatattaatg ttacatcagc acctctagct atagcttctc ttgctataga
      901 atatcccatt ttaccagaag acctattagt taaatatctt actgggtcta tactctctac
      961 agttggacca gctgtaacta tcatactttg accttttaaa tcttgctctt tagtaagatt
     1021 agtcaaaact tcctctacta ttatatctgg attagctagt ttacctgttc caatatctcc
     1081 acatgcaagc cttccacttt ccggttcaat aaatttatat ccaaagctct ttaaagtttg
     1141 tatatttttt tgaacaattg gattttcata catattagta ttcatagctg gtgctataag
     1201 aacctgtgct tttgttgcca taacagtagt tgtcaacata tcgtcagcta taccatttgc
     1261 aatcttgcct ataacatttg cacttgctgg cactattaag aataaatctg ctttttttgc
     1321 taatgatata tgttcaactt cccaagtttt tggttcttca aacatatctt ctactacata
     1381 attttgactc agcgattgaa atgtaagtgg tttaacaaat tctgttgctg attttgtcat
     1441 tataacatgg atatttgcat ttagtttttt taacttactt acaacatcac aagccttata
     1501 tgcagctata ccgccactta tgccaattac aacagtttta tcttttagca tgaccttact
     1561 cctcttctga aatttgttgg tgttgctcag catgatgtct agcttcttct atctctattt
     1621 cctcttctgt caataatcta tatgttattt ttccacttgc aacttcttta gttgctacac
     1681 atacaggctt ttccttagtt ttatttgaaa catatggttc ttctccatca attaattttc
     1741 ttgctctctt tgaaacagtc cctactaaat agtatctgtt gtctattttt tctagtacct
     1801 cattgataga tggttttaac ataattataa ttcctcctta aatttctcta ttataatatt
     1861 tttatatctg ctaactttat tcttttcaga tgatattata ccttctattt ctttagcaga
     1921 tgtttttaca tcttcattaa atataaaata atcataatcc ttaatcattt taatttcttc
     1981 atatgcacag ctaaatctct tctctatttc agcttctgtt tctgtacctc ttccaacaat
     2041 tctatttttt aattctttaa gtgatggtgg tagtacaaat ataaatactc cctctgggca
     2101 aacttctttt atttgttttg ctccttgcat ttcaatctcc agtaatacat cttgtccttt
     2161 ttctaaacat tccattattg ccgcttttgg agttccataa aaattatcat aaatttgagc
     2221 atactccaaa aactcacctt tttctatcat ctcttcaaac ttttccttac ttatgaagaa
     2281 ataatttact ccatcaactt ctccagttct gggttttctt gtagtagctg atacagaaag
     2341 ttttattgta tcattttctt taagtagttc tttgcaaata gtaccttttc ctgcacctga
     2401 tggtcctgat acaactagca ataatccttt tttcacaaac atttaagcat tccccttcta
     2461 atattgtatt cttactctat attttgtatt tgttctctta ttttttccaa ttcgcttttt
     2521 acttctacta ctaaattagt tatatttata tcagatgact tcgaacctat agtgttagtt
     2581 tctctattca tctcttgtat taagaaatct atttttctac ctatagagtc gtctttaaat
     2641 atagtatttt taagttgccc tatatgactt ttaaatctaa ctatttcttc tgttatactg
     2701 cttttatctg catatattgc aacttcttgt gctaatctat tctcatctat tatacttggg
     2761 tcatccagta attcacttat tctcaaattc aatttttctc tataatcttc tacaacacta
     2821 cttgaatatt tttcaatctc ttctatatga ttttttaata aatcacatct catggcaata
     2881 tcttcagcta attttttacc ttcttcactt ctcatttctc ttaatttgat taatgcatct
     2941 tcaactgctt gattcaacat tgaccacaat aaatcttcat cttcttcctt ttcttctatt
     3001 ttcacaatat caggaaactt tgcaacattc ataacgctaa tatcatctat aatatcaaat
     3061 ttatttttta tttcttttag tatgtctata tactgagatg ctaatccctc atcaaaattt
     3121 aagtttacat cttctttgcc taataagtca aactttatgt ataaatctac tcttcctctt
     3181 tttacataat tttttactaa atttctaact ttatcctcta aaaatgaaat ttttcttgga
     3241 agacgtatgt ttatatctga atacttatga tttatcgttt tacactctac taaaaagtaa
     3301 taattatcat ccttgtattc tcctcttcca aaaccagtca tacttatagc cattaattaa
     3361 acctccaaaa taccttcaca tatttttact gctggacctg tcatataaga gaaatctcct
     3421 acatctattt taactgttcc accttgagac gttacattta cactactttc taccatatta
     3481 agtaaattgc acactattgc tgatgcagtc ataccagtac cacaacctaa agtatatcca
     3541 caacctcttt cccaagtata aacatgtata ttatacttat cttctacctt tacaaaattt
     3601 acattcgttt tttcaggaaa tattttatta ttttcaatct cttttccata tttacaaaca
     3661 tcatctacat ctaattcatc tactataact actgtatgag gaacccccat caacatagaa
     3721 gtaactttaa gctccctatc taaaaccttt atactttctt gcacaaaacg ttctttattt
     3781 gtaagtacag gaacgtcctt agctataaaa cttccttttc ccatattgac tcttatagag
     3841 tttatttctc tattagtaag atttactttt atatctttta taccatctaa tgtatctact
     3901 gtaaaacttt cttttttaac tatacaatta tcataaacaa atttacaaaa acatctaagg
     3961 ccatttccac acatagctgc tcttgaacca tcagaattat aatataccat ttctacatct
     4021 gcaacttttg aatctttcac aactaacaat ccatccgctc ctacagaaaa tcttctatgg
     4081 catacttttt ttgctaaagc agtataatca gatggattta tctggtcgaa tcttccatct
     4141 attgctataa aatcatttcc tattccatgt agtttccaaa atttcataag tatcgctcct
     4201 tcgtaaatta acacttaaac tattataaca taaaaaaatt cccataaact tataatttat
     4261 gggaaaagtt ttgtaatgac aactataatt tgaggatgtg agggagagtt gccattacaa
     4321 ttagggtaat tatattatgt ttttaagcta gttctttcta tgtttatata ttaatatttc
     4381 tatatgaatt ttatgtatgt tttatttaaa ctgtctatga attttttaat taaacatctg
     4441 gtaactagct ataatccttc tagtagatat ctattaacat acacctatta attctttaag
     4501 tgtttttaaa acatagtcta aatcttcctt agtaatatct acatgagtta caaatctata
     4561 ttcaccattt tccattttat taatttttat atttctttca aaaaattctt ttattaaaac
     4621 atcctctttt ataatatctt cactcatctt aaaaaatacc atacttatat ctggattaac
     4681 attgtttact tcaatgccat caattttatt taattcacaa gctaaatatt ttgcattttc
     4741 atggtcttca ttcaatctta aagtcatttt ttcaatggca attataccag ctgctgcaac
     4801 tatacctact tgtctcattc ctccacccat aactttttgc tttcttctag cttttgctat
     4861 aaattcttta tttccagcaa gtatagaacc cattggtgca gctaaacctt ttgacacaca
     4921 gaacataaca ctatcacaac actttgctat ttctttagct tctacaccca aagttaatgc
     4981 tgcgttaaat aatcttgctc catctaaatg tactggtaag ttatgtttct cagctatttt
     5041 tttaatttct cttaaattat caagactcac aactgttcca cctgaatatg cattttctac
     5101 acatataagc cctgtctgtg gttcatgtat gtcatctctt ctaattgcct tttcaacaag
     5161 ttcaggattt agagctccac tttcgtcttc atctatagtt ctaagctgaa ctccagctat
     5221 tacagcaggt gccccaacct catgagtcac aatgtgataa cctttaccaa taattatttc
     5281 ttgccctctt aggcaatgag taaatagagc taattgattc ccaaaagttc cacttggaag
     5341 gaaaatagca tcttcttttc caactttagc tgctgctaat tcttcaagtt tatttacagt
     5401 agggtcatct ctaaagattg aatctccaac aggagccttt gccatagctt ctctcatttc
     5461 gtcagtaggc atagtaacag tatcacttct taaatcgata aatctcattt tttcaacctt
     5521 ctttcatatt tatatactat agaaccttaa aattgatttt ttctgtaaag ttcatatact
     5581 ttcatagctt atactctaca caataattat actatattta ctttatattt aatctacttt
     5641 aaattattaa ttttaaaaat aaagtttcct ctctaataaa tataaataat tatacaattt
     5701 aaatattaat ctctattgtt taaattcccc ctagtcacaa agcgtatatt atgtgtataa
     5761 ttaatttact gactcatttt ggtcaaagaa aggggtgcta taaatggcat tagatggatt
     5821 agttatacat tcaatagtcg atgaattatc atcaaaactt acaggtggaa aaatagataa
     5881 gatacatcaa cctgaagatg atgaggtaat ttttaatata agaaataata aagaaaattt
     5941 tagattggtt ttaagtgcaa gtgcctcaaa tcctagagtt tatcttacaa gtaattatca
     6001 aaaagaaaac cctctaaaag cgcctatgtt ttgtatgtta cttagaaaat acatacaagg
     6061 tggaaatata gtagaaattt ctcaaatagg ttttgaaaga ataataaaaa taagtgttga
     6121 atctttagat gaattaaaag aaaagacagt taaaaatatt atgattgaaa ttatgggaag
     6181 acatagcaac ataataataa ctcatggtga agaaaataaa attatagatt ctataaaaag
     6241 agtccctttt agcataagta gagttcgtca agttttacct ggtcatgatt attcattgcc
     6301 accagaacaa aacaaactca accctcttga tgatatatct aaggatttgt ttattaaaaa
     6361 cctagaggaa ttggaaggac ctatctttaa gtctatatat tctaaatttc taggtataag
     6421 ccctattata gctaaagaaa tttgctacag agcaggtgta aatcaaaatg ctatcataaa
     6481 ggatatatct gatgaacaat ttgattcttt acacaaagta ttttgtaatt tattcaatga
     6541 tataaattct aacaaatact ctccttgcat tattatagat aaaaaggtgg ataaggtagt
     6601 tgatttcagt tgtataaacc ttacattatt tagcgattta tcatatataa ataaggatag
     6661 tatgtctcgt attctggaag atttctatag aactaaggat attaaagata gaattaatca
     6721 acgttcatct gatttaaaga agagtatatc tgttaagctt gatagacttt ataataaact
     6781 taaaaaacaa gaagaagaat tatcagaatc cgaaaatgca gacatttata agataaaagg
     6841 agaattgatt acttcatata tttatatggt agaaaaaggt atggaaagta ttgaagttgc
     6901 taacttctat gatgaaaatt gtaatgatgt aacaatagaa ttaaataaaa acttgacacc
     6961 atctgaaaat gctcaaaaat actttaaaaa gtataacaaa atgaaacatg ctaaagtcga
     7021 gatatctcat caaatttcct taaataagga agaaatagat tacttagaaa acataatttt
     7081 aagtattgaa aactgtgaaa atttagctga attacaagat ataaaagaag aacttgctaa
     7141 ggttggatac atcaagactc aaaagaaaaa cagtaaaaag gatactatcc catctacaaa
     7201 acctcatgaa tttgtttctt ctgatggatt taaaatattg gttggtaaaa acaataagca
     7261 aaatgactat cttacactaa gacttgctga taatgatgac ttatggatgc atacaaagaa
     7321 tattcctggt tctcatgtaa taataaagtg tgctggtaag gaagtccctg ataatactgt
     7381 atttgaagga gctatgctag cagcattctt tagtaagtca aaactttctt ctcaggtgcc
     7441 tgtagactat actaagagaa aaaatgtgaa aaaacctagt ggttctaaac caggtatggt
     7501 tatttatgaa actaacagta ctatttatgt tactcctgaa gaagaaacag tagccaagct
     7561 taaggttaaa tctgaataaa ttctgcacta aatacaaaat aaatgttaga caactacaca
     7621 ttatcattct aaataaaaat agtggccttc ttataaagta caataataag gccactttct
     7681 aattagctta cttttaattt tctaacaaaa tacttttctg ttttaataac tatacttata
     7741 ttcccaacaa atattttcag tcttcttata tagatgtaat taaaaagtta acactaaaag
     7801 ctatttttta atttattgat ttgttttagt aattcattat attctgattc atacaattct
     7861 ttttttagat tatctttttc tatagataac atagatataa cagttgtaag tctattttct
     7921 aatactagaa gattttcttt tttgatttgg ttttctctac taggagtttt tttatttctg
     7981 ctaattgtat attcgtcata attacaatca aactccctta ttttagcatc ttttatctct
     8041 ataatatagt tacaaatatg tgatacaaat actctatcat gagacactat aagcatagtt
     8101 ttatcagtat ttatcagtgc cttttccagt gcttccatag attttatatc taagtaattt
     8161 gttggttcat caaatactaa aaaattatta tcttccaata ttattttaca tagtgctatt
     8221 tttacctttt ctccaccact aagtactttt acttttttgt agacatcatc acctttaaac
     8281 ccaaataggc ttaagtttat ccttataaaa gattcatcaa atgaactatt aactttagta
     8341 ttgtatagaa cactcttttc ctcatctaat aaactttgat tttggtcaaa ataacctact
     8401 tttaccttgt tatttatttt tatattgtca tttttatcag ccaatatttc ttttatcaga
     8461 gtactttttc cacacccatt atcacctaat aatgctatct ttttacctct ttttatctta
     8521 aaggaaacat tatctaacag gagcttattt tctaacttta gagtcatatc ttttacctct
     8581 actagatttt tactaattat ctccatacca tcttgaatct taatgttcat ttctttagaa
     8641 actttaggtt tattctttac ctcaagttta tcaattctgc tttttatagc cttcacattt
     8701 gagtccaact ttttcttatt agtctgacca cccattttgt gcaacctagc ttctgaatta
     8761 cccattcttt ttggtgtttt tcttatgcca tcacttaaag cttttttata taaaagagct
     8821 ttttcaagcc tttttttctc tttaacatat tcttgatact caaaatcagc tctttttact
     8881 tcttcatctt tttgcattaa ataatcagtg taattgcctt tgtaagcttt tagttttcca
     8941 tcttctagtt ctaatatagt tgtacataac tcatccagaa aacgtctatc atgagatatt
     9001 aataataatg ctccctcatg ccttttaaac atatcttcca aaaccccaat gcttttttta
     9061 tctaagtttg aagttggctc atcagctatt atgatatttt tcttattact cagcgcatct
     9121 gctattttta acttaacctt ttcccctcct gataagtgtt cttcaaattt atctggagca
     9181 tttaaaagac tcttctcctt actatgacta catgtttcaa tattattttc actctgactt
     9241 atataagaat agctttctgt aaggtaagta tatccttcat caatttctat ttctcccaat
     9301 attgctttta ataatgttgt ttttccttga ccatttgaac caactagacc tattttgtca
     9361 ttttctaata tttcaagttt atctatatcc aaaattaatt tatctgcata atatttcttt
     9421 aaattctcta cttttactaa caacataaaa aatccctcct taacacactt aaatataagg
     9481 agagattcta taaataggca taacaaataa acgtatagac tataatctac tctccttaat
     9541 ttaagcattg caaataaaac caccaaaata ggcttttcaa aaattacaat actttaaatt
     9601 ataaaaaata gattatattc tcattgtcct agttaccttg ccctttcttt catttatttt
     9661 taatcattat atcaacatta attacaaatg tctatagaaa attaactaat tttagttatt
     9721 caatatacaa cttattcttt aaatttattc ttcttcaact ttaaagtcct gattatttaa
     9781 aattctgttt aatacaattc caacaattgc agctaaacta agaccagtta tctttactgt
     9841 acttgttata gggattccaa cagaaatacc tttattagat aaatatgttg ttccaagacc
     9901 tattaataat ataactgcta tgattaatat atttatttta ctttctacac aagaactgtc
     9961 ccttatagtt tttaatccta cataagttat catcgaaaat agcattatac taattccacc
    10021 cataactgaa cctggtatac tttgaagaaa tccaccaaac ttgcctacac atgcaagcaa
    10081 tatagcaaat attgctgtac gtctaagtat tgaagggtca tagtttttag ttatagcaag
    10141 tactgctgta ttttctccat aagttgtatt tgctggtcct cctaaacacc ctgcaactat
    10201 agtcgcaaat ccatccccaa gtaatgttct atttaatcca ggattttcta taaagttttt
    10261 acctactaca gcaccattag tagtcatatc tcctatatgt tccataaata cagctaatac
    10321 aactggagat ataatcacta ttgctcctaa actgaactta ggaactgtaa agttaggtat
    10381 tgcaaataaa cttgcttctg taatagtagt tatatctact aatcttaata tcaaacagat
    10441 tatataccca gaaaaaactg ctattaatat tcctaattgt tttataaatc ctctaccaaa
    10501 cttattggtt aaaattgcta ttgctaaagt tataaatgct atcacaaaat tggctgaagc
    10561 catactaaaa gctgttggca ataaatttaa tcctataact actatcatag ctcctgtaac
    10621 ttgtgcagga aaatataatt taattttatt tacaccaact acttttacta taatcgacat
    10681 tataatataa attaatcctg ctactattat accaccttga gcatatgcta aatctccacc
    10741 atacgcctct ttagctgcta gtataactgg tataaacgca aaacttgaac ctaaaaaggc
    10801 aggtactttt ccttctgtac aaaaatgaaa tatcaatgta ccaacaccag cacagaaaat
    10861 tgcaactgac ggattaaaac ctgtcaaaat gggaactaat actgtcgctc caaacatggc
    10921 taatagatgt tgtaaagaca ttatcgtttt tttcataaaa acctccaaaa ttattatata
    10981 taaataaatc ctctgtttaa ttaatacctt ttaattaatt atcattccct agattctatt
    11041 ttttaacctc tatatactta ttatataatt tatcattatt ttacatttta cgacatcaaa
    11101 aactcaaata aaggagaata aaaactacat taagactcac atccataaaa ttataatatt
    11161 tattttgtgc aatctatgta agctttatcc tcctccctga ctattcaatg ctaagctttt
    11221 atataatact taaaaataat tattctttga ttgtaactga atcttctcca tcaatttcta
    11281 ataaactgac agatataatt tcatgtctag aagtaggtac attttttcct acataatctg
    11341 ctcttatagg caactctcta tgccctctgt ccactagcac tgctaattgt attgatttag
    11401 gtcttcctat atctataata gcatctagtg aagctctcac agttcttcca gtgtataaaa
    11461 catcatcaac taaaactact gtcttatcat ttacattaaa atctaaataa gttcctttaa
    11521 ctacaggttc aacatgaatt ttttccaaat catctctata caaagttata tcaatatctc
    11581 ctgtatctac ttttgttcct tctatttgtt ctattttctt agaaattcta tttgcaattg
    11641 gcacacctct agtctttatt ccaactaaaa ctagattttc tacaccttta tttctttcaa
    11701 ttatttcatg acttattcta gtaattgctc ttgctattgc cttttcatcc attaattgag
    11761 ctttttctac catatcataa ttattgtcaa agatataata tatctatttg acaaatctac
    11821 tcctttcttt taatttttta taaatttatt tttatttaaa atttcaattt aaacaacaaa
    11881 aaaacttctc atcacaagac aagaagctct tatataaaaa atatacagtg taatttagca
    11941 attgtcttac gatatctctg tatcgtttaa agataatttt ttaatctatc aactcaaatt
    12001 atatatttat atttcaaatt tttcaagctt ttttaaacaa aatactttaa tataagatat
    12061 tttatttaaa tttcactaaa tttatttact tatataaaaa caaaaaatga tttataagta
    12121 ttatacaaat ggttatttta tctttttaat aacttcctta aaatactcag gtaattctga
    12181 attaaattcg atatattcct tagtagtagg atgtataaat cccaacttct tagcgtgcaa
    12241 agtttgtcct tttaatttaa atttagtttc ttttggacca tatacataat cacctaaaag
    12301 tgggtgattt atagacaaag catgaactct tatttgatga gttctaccag tttcaagcct
    12361 agctctcata tgtgtaaact tatcaaatct ttctacaact tcaaagtgag tgattgcatt
    12421 ttttccatct tttactacag ccatttttaa tctatcctta ggatttctcc ctataggttt
    12481 atcaacagtt attttatctt ctttaaccac accatagcat ataaattcat actccctagt
    12541 tatagtatgt tcctttaact gttctgctag aaaattatgt gcataattgt tcttagctat
    12601 cataagaaga ccagaagtat ctttgtctat tctatgaact ataccaggtc taataactcc
    12661 atttattgat gacaacttat ctttacaatg atataaaatc ccatttacta acgtattttc
    12721 atagtttcca ggagcaggat ggacaaccat atcctgaggt ttatttatta ttaagacatc
    12781 atcatcttca tatacaatat ctattggaat atcttgaggt aaaacttcta atactttagg
    12841 ttctggtatc tctataacaa tattatcttc ctctttaacc agatatctag ctttttctac
    12901 tttattatta acctttactt ttccatcttt tataattttt tgtatataac ttcttgacat
    12961 atcacctaat tgttctgcta gataaatatc aagtctaact tcttcttcct cttcaagtac
    13021 taaaaactct tttacttcat ccattatatc acctactttt actttcaaaa aataaaacat
    13081 atatacataa aaacacagtt cctacaacta caaatacatc agctacattg aatacatatt
    13141 cccatataac tctaaagtcg aagtaatcta ccacaaaacc taatcgtact ctatcaacta
    13201 gatttcccaa tgcaccagat ataattaata taattcccaa ccttccaaat atatgtactt
    13261 tccttgtatt aagatagtat aatccaaata ccgttgcaag taatgcaaca attataaata
    13321 tccattgatt attttgtaat aatccaaatg ctgcacctct attttcgaca taagttaaat
    13381 gaaatacatt atttattatt ggtattgttg atacatccac caaattattc aatacccata
    13441 ttttagacag ttggtctaaa cctatgagta gaattattat taatatatat agcaatgatt
    13501 tttcccccta taaattattt aaaaaccaat tttatttgaa gcacactatt attattatac
    13561 aaaaaaaatg ctctaaagag caactttatt aaaattgtat tttcttaata aagctagaac
    13621 aaaatttaaa atagtactac tttttttata ttgtagattt ctacatattt aaaatcatat
    13681 cattatgtat aaaaaattta aaaataccat actaatcaat attttttgtg tattttatag
    13741 attaaagatt aatgatgtct gtaatttaaa cttacaatca ccactaatct tcaaacaaat
    13801 atttttactt ttgcacttgc ttatttttat ctaaatgttg tataagttca gatactgtta
    13861 cgaatttata tcctctctct tttaatttag gaattactac ttctaaagct tgtatagttt
    13921 gtgatttatc atttctttta gtgttataat catgtaaaag tattatatta ccattttgga
    13981 cctttgtaag gatagtattt ataattgcat ctactccagg gttagaccaa tctctagggt
    14041 ctaagtttga ccaaagaatt atattcattc ctttagaaac tataatatca cagacacttt
    14101 tactcatggc tctatatgga ggtctaaata tctttggttc tactcctatc acacttttta
    14161 taatattttg cgtatctgtt atttcttttt caacactact ataactattt tttgcaacat
    14221 ttatatgtgt aaatgtatga ttccctattt catgcccctc atcatattgt ctttttaata
    14281 attctttgtt atattctact ttttctccaa ctacaaaaaa agttgctttt acatcatttt
    14341 tctttaatat atcaagtact tgtggtgtaa aatcctcatc tggaccatca tcaaaagtca
    14401 atgctaaaat tttttcatcc tcatttcctt tttttattat tacatcttca taatcgtctt
    14461 ttatattgtc atttaaacta tatgcttctt ttgaactatc ttgattatgt gtattatgat
    14521 tatatgtata tacacctact atagataata aacatataca tataccacat attattttct
    14581 tcatgtcatt acctcttttc taattcattc tagcaatata ttttataatg tcagtttttt
    14641 aatctctcaa tattccattt ttatttaaat cagttaaatt tgttaggtct tctgaataaa
    14701 agttagaatc ttctacatca gggttttgca ttgcatcacc taataaatta ttttccttct
    14761 ttgcacaatc attacataaa cttgtatctg gcaatatatc caatctttct aaatctattt
    14821 gattatgaca taaatcacaa atcccatatg ttccattctc tattttagat aatgctaagt
    14881 ctatttgata caatcttccc tcttcatgat ttattagact gttttgcata ttttgcatat
    14941 ataaatctgt tcccatgtct cctatatgat tatcataact agataattct cctgatgaat
    15001 acttttcact tgtatgttta gttgtatctc caaataaagt attatctttc atatcctcta
    15061 ctaaattagt taaattttct ctttctttca ataatttatc tttatatttt ttagtatcca
    15121 tttttaacac ctctaatttt aatatttaca tcataaaaat agtatgtgga aaagtaaatt
    15181 aaacatacaa taaaaataag atttgattcg aaaaatggaa tgtctcaaaa tgaaattttt
    15241 agttcattta accctctttt ttatatgaaa tcaaatatat ttttatcctt tcagcaataa
    15301 aaaagagact tatctctatt ttgagatagt ctcttctttg aacataattt aaatacaatt
    15361 ttttaatttt tatagttatt attttactaa cttgccaatt tatgattatt ttgttcttta
    15421 atatgttttg cttttttaaa gaataaaaga ccaagaatta ttgctacaat aacacctatt
    15481 ccattagata tattaactgg tagtctaaat ccttcttttg cttgcattat ataactaaat
    15541 gttactacag tcataaaaag tgctggtaat gtagttatat aatggttttt atttgctttg
    15601 actaaatatg cagaaccagt ccacaacatt atcattgcaa gtgtttgatt tgcccaagag
    15661 aaatatctcc aaattatatt aaaatctata aatgtaagag caacaccaac tgcgaataat
    15721 ggtaaagcta ttaaaaatct atttttgact gcatcttgat tataattcat tgcatctgct
    15781 attgtcagcc tagcacttct aaatgctgta tctccagaag ttataggaca tgccacaact
    15841 ccaagtatag ctaaagctcc tccaataggc cccattaatc caacagatac tgtatttact
    15901 actgttgctg ctccaccatc attaaatatc acattaagtt gaggtatgcc atggaaatat
    15961 gacatggctg ctgctgccca aactaaagct attatacctt ctgcaaccat tgacccataa
    16021 aatactggtc ttgcctcact ctctttctca acacatctcg ccataagtgg tgattgagtt
    16081 gcatgaaaac ctgatattgc tccacatgct attgttataa ataagtatgg gaataaaggt
    16141 gttccatttg gattcatatt ttgtaaattt atatttggta tgttataccc ctttattatt
    16201 aatcctccag ctacacctac tgccataatt aataatgctg ctccaaataa aggatatatt
    16261 ttaccaatca atttatctat tggtaaaaca gtagccataa tataataaag tattatcact
    16321 ccaacccata tttgtgcgtt tacacctgtc aatgtcttta ataatcctgc tggacctgtt
    16381 acaaatacaa ctccaaccaa tactaaaaga ataacactaa atattctcat tgctattttt
    16441 gcaccatttc ctagataaag gcctaccaac tctgatgcag atgtaccttt gtttttcatt
    16501 gatagatatc ctgaagcaaa atcgtgtact ccaccagcaa agattgttcc aaacacaatc
    16561 cataaaaatg ctgatggtcc aaataatgct ccttgtatag ctccaaatat cggacctaac
    16621 cctgctatat ttagaaactg aattaaaaag attctcttcc atttcattgg cacataatct
    16681 actccatccc tacaagctat agctggagtt tgatttttgt cattggttcc aaaaaccttt
    16741 tctacaaaag taccatagac aaaataacct actataagta ctactattga acctaaaaag
    16801 cttaccataa attcatcccc ttagttttta tataattgct ttataaattt ttatgagttt
    16861 aagaaaacta tttccagttc aaatagtaat acttttttat gctaatataa atattaatta
    16921 cattaagtgc atatttaagt acatgaaatg actttatata tgcattaaca gcctaaattc
    16981 ttttactttg cttctactta cagttacttc ataatcacta ttttttaatt ttaatatgta
    17041 tgtattatta aaccatggta taatttcttt tactttggtt aagtttacga tataagacct
    17101 atgtgttcta aaaaatgttt tatcatttat aagattttcg acttcttata tgccttcttt
    17161 taatataaac ttttctttct cagtatatat aaatgtctgc ctttcattag cttcacagta
    17221 atatatatca tcaatatcta ttacaactaa tttatcacct ttccaaaggc taattttatg
    17281 tgttatttct tctatctcct gatttactgc ttccttctta tatttgtata aattactatt
    17341 tacattaaat agttcaatat cactcatctc acttttttct aatttattca gcatagaaat
    17401 aattctctct tcagaatatg gtttcaatat ataatcaaat acatccaaag aaaaagcatc
    17461 tactgcataa ctttcatatg ctgttataaa aacaatttta ggtcttgatt taaactgatt
    17521 taatgttttt gcaagtagca ttccatcaag atgaggtata tttatatcta aaaaaattac
    17581 atctatttta ttttcctgaa taaagtctaa cacctctata ccattggtaa attcacttac
    17641 tacctcgatt ccacttttat tttcaataaa atatctcaat tcctttcttg ctggaaattc
    17701 atcttccact attatagctc tcatacttct actcctttac ataaaatact atcaaagtcc
    17761 cattatctat tcttcttata ttaagccctc ttccatacat cagtttaagc cttaaatgta
    17821 cattataaag cccaatctta ttttctggca tattatcttg atatacatta tttataattt
    17881 cctcacttat acctatacca ttatcctcta ttgatattct cacagtattt tctaatgaat
    17941 acggtaactt ttttatggat attttcacta caccagctct accactttct aaaataccat
    18001 gtattattgc attttccact aatggttgta taatcaaact tggaatcttt atatttacat
    18061 cttctatatc ataagataca gttagtaatt ctccaaacct agctttttct atctctacaa
    18121 aagatttaac ttgttcaatc tctttattta tatcaattaa gttatcacta tattcaagat
    18181 tatatcttag ataagttgat aaatttatta ttaactctct agccttatct ggactaaatc
    18241 ttataaatga agctattgtg tttagggaat taaacaaaaa atgtggatta atttgtcttt
    18301 gaagtgcttt tatttctgct ttatttctcg cttctatatc ttttttatct ttctcaatcc
    18361 cctctactat agatataaca aatcctattc caatctgccc aactattaaa ggtaaatata
    18421 ttccaccgat tatttgaatt gctaaagaat gtggatatga tattaagtat ataagtaata
    18481 tagtaaaact ttcagaaatc atgcctacca atattccata cattactcta tgttgttttg
    18541 gtatcctctt atatagaaat cctgataata cacctgctag tatacttgat aaaagacatg
    18601 gaacagatgt tatcccccca atatccataa aatatctatg aatccctgaa aaaactcctg
    18661 ctgtaatacc taccatcgga ccaaataaaa tgccacctga tactatagat attattctag
    18721 tgtttactat tgaccccata taatttatac cactataagt tccaaatatg gctaaaaaag
    18781 taaaaactaa tgctatacag accaagtcat taaaactata ttcttccttt tgaaatatct
    18841 gcttaaatat ttttaacttt gttattagaa gaaacaatac tacaataagg cttgtctttt
    18901 ctattaatgc gattaaaatt gatgttttca taatccccct ttaaaaaata aatttttaca
    18961 cactctaaat ttattatggt tgttataaat tacatatttg aagtaatcct atcacaaaac
    19021 tttccacatt taatctttta attaaataag aaatagttgt acttctatta acaactaata
    19081 ctagctagca taagcttgta ataacttgta gttattatta actttacaat tctagtattt
    19141 aattttatac tttagaaatc tcttttctta gaaataatac tttcgaatat catataatac
    19201 aatattatgt agattcttac tcatacaaca tatttcttta aaaattttat actattcttt
    19261 gttcaaatat ctcatttgat ttaattgtgt taaacacaca ttatcatctc tctgttatat
    19321 atttattata tagccg
//
LOCUS       DAYATN010000065        19096 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.065, whole genome shotgun sequence.
ACCESSION   DAYATN010000065 DAYATN010000000
VERSION     DAYATN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.065"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(146..682)
                     /locus_tag="ACGI8A_002191"
     CDS             complement(146..682)
                     /locus_tag="ACGI8A_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFH0180187.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(700..2013)
                     /locus_tag="ACGI8A_002192"
     CDS             complement(700..2013)
                     /locus_tag="ACGI8A_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFH0180188.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2368..2808
                     /locus_tag="ACGI8A_002193"
     CDS             2368..2808
                     /locus_tag="ACGI8A_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HFH0180189.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2992..3180)
                     /locus_tag="ACGI8A_002194"
     CDS             complement(2992..3180)
                     /locus_tag="ACGI8A_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0180190.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
     gene            4228..4623
                     /locus_tag="ACGI8A_002195"
     CDS             4228..4623
                     /locus_tag="ACGI8A_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HFH0180191.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4666..6765
                     /locus_tag="ACGI8A_002196"
     CDS             4666..6765
                     /locus_tag="ACGI8A_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFH0180192.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNTSTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(6990..12200)
                     /locus_tag="ACGI8A_002197"
     CDS             complement(6990..12200)
                     /locus_tag="ACGI8A_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFH0180193.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSMDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12215..14710)
                     /locus_tag="ACGI8A_002198"
     CDS             complement(12215..14710)
                     /locus_tag="ACGI8A_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0180194.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15371..15658)
                     /locus_tag="ACGI8A_002199"
     CDS             complement(15371..15658)
                     /locus_tag="ACGI8A_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180195.1"
                     /translation="MRLNITKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15678..15953)
                     /locus_tag="ACGI8A_002200"
     CDS             complement(15678..15953)
                     /locus_tag="ACGI8A_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180196.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16608..16787)
                     /locus_tag="ACGI8A_002201"
     CDS             complement(16608..16787)
                     /locus_tag="ACGI8A_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="HFH0180197.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(17072..18547)
                     /locus_tag="ACGI8A_002202"
     CDS             complement(17072..18547)
                     /locus_tag="ACGI8A_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0180198.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
BASE COUNT         6355 a         3332 c         2085 g         7324 t
ORIGIN      
        1 gagtataatt ataaaaataa aaaaagaata ggataatttt cctactcttt tttctgtact
       61 tacatataat attaaaactc tttatttttt aatatatatt ttaaattttc ttttttaata
      121 aatctttttc ttttaataaa tctatttaat tttcttcttc tatatcagag ccaaagggaa
      181 tccatgcatt accaaactct ttatctttaa ataaataagc taatccagtt atttgcttca
      241 atctaagaat ttcatcaact tccataccca ggtgctttga tatccattca tcagattttc
      301 caatttcaca aagttcagtt ataattttac tcattaaatc cacattatga gaacctcttg
      361 cacgattgtg tcgaatagta cttgccattc tttcatcaag agatttatca attactgaaa
      421 caggaagaag tccattttct ctatcatata tatcctcatt ttcaagcatt actctatatc
      481 tatgaaaacc atctacaata atatacttat ccttttcctc attataataa cagacaattg
      541 gcattgtata cccatcttct tttatacttt tatacaacaa ctgcatttct ggtggggcta
      601 ctgcatttgg attatagtcg ttagattcta tcttttcgat tggcactgcg atgattccat
      661 atacaggact tttatattcc atagcatctt aactcctatc taatgaattt tttcatattt
      721 cttcttaata tattccgttc taattctctg ttcttttgta ggaccaaatc ccataaatct
      781 acacatatgg tcatttttta aaatacaata acacattctt ttccaacttg gtatatcttt
      841 ggtaaattta atatcatccg tatcatcagg tatatcatcc aaaaatatga ctctactctt
      901 tttatctttt gtatgatttg aaactccatt taattctatt ttataacctt ttctcaacaa
      961 ttcacgtata actatttctg acaaaccacc acctgtattc tgccaaaact caatagatgt
     1021 tgaaaatttt ttaatatagc tattccttat attttcaggt aaggtatcaa gcaaaaaata
     1081 tgtgaaactt ttccatgtgt gtccatatgg caatttaaca ctacgatatc ccatagcttt
     1141 tgttttgcca taaatagaag taaaattaac tccttgtact ctagcaacca atttcaccca
     1201 tacatctggg tccaatattc tgtataaatt taaactctct tttgctgaat cattaaaagg
     1261 tgatgccact ctcatttttc ttggagctac tccagcttta tagaataagt cataaatttt
     1321 attatagtca tagtcaaatt tatagtgtgc taaccatata tcttctattt cccaatcata
     1381 tataggagat gccgtccaga catttttaaa ttccttagtt atccagcact ttcctttata
     1441 tccatgctgt ttattcacaa tagaacaata acgttgtaga gattctgctg ctcgtgttcc
     1501 taataagcaa acaacttttg caccttcatg tatatctcta atccatctac caaattgttt
     1561 tgccaaactt tcttgtctca ttttatattt ataataagta aaaggattat ttttttgatt
     1621 tattatgtat tctttgactg gcatttccct tacccataac tcttctttct catcatccca
     1681 tggataccaa tacatttcat aatttcctac agcagttctt gtagccattg gtaaacacac
     1741 ccaatatggt tcaatatctt caatattatc ttcaaatact ttttcaacat attcagtagt
     1801 caaactatat tgagcctcaa aatcttgatg aaatactcct attttggaac ttatattatt
     1861 ctttctctta taatccaaaa ctaaatttag cagtaaacca ctatcttttc ctccgctaaa
     1921 agatacatag atataatcaa attcattaaa aagataagaa agtctttctt gaaaagcttc
     1981 aaatacatcg gattctaaat atctcttatc catttgaatt atcccctttc aattagaagt
     2041 ttataaaacc ttacaatgca atattattat gcttttttag cagtaatcag cataaattat
     2101 acattattta aatacacaaa tccaactaac tacatataat tataatgtaa ttatactttt
     2161 gtattataaa ctatatttta tcattatttt acattttttt ccaatatata taaatatcat
     2221 atttttctat tagctaaaac taattacttt atatttctac gtattagtat aataatacat
     2281 aaaaacttga ataatgcata aaaacttgac atactgttgt cataattact ttgctataat
     2341 taaaagtaaa aatggaggta aattctaatg tatgaacgta tgcttgataa aagcaataaa
     2401 ccctgtttgt cccaaataat agaatatata ggtaaagatg gatatgcacg attgtgtaag
     2461 ttggaaactt acttgaaaac tcaatataat ttatcaagag aacttcgatt tccttttgga
     2521 aacaattatg gttggggtta caaatatagc cataaatctt ttcatttatt ttatgctttt
     2581 tttgaaaaag gtgcatttac tgttctgatt cagataggtg acaagcaagt tccagcagta
     2641 gaaaaaacgt tggtagcttt gacaccaaaa gctaaagaac tctgggtcac tcgttatcct
     2701 tgtggcaata atggaggctg gattaagtat cgtattttag aagatgcaga cttacttgat
     2761 attattcaat atataagagc aaaaaaatct ccacctaaac aaaattaact tttatttttt
     2821 gtgatttaaa tatagtgtat ataaattcca ttctttccat attaaaaaat aatataaaaa
     2881 atatctatta gtataactta tttagtatgt cttattctac tttttacaga taattaaaaa
     2941 gttttatcat aaataaatca taactagaca tcctagtaaa tcaaaattaa tttattcttt
     3001 tttattcgaa tttttgataa tagtccctat acatatgcca attatcattc caaaaattag
     3061 accataacac attgtaatac ctacatttcc tttagaaata cctatagcac aacctatagc
     3121 cattccaagt atcatgccta taaacataca tataataact aaattgttgt ttttattcat
     3181 agtttttctc cattcaatat attttaaatt gcctagctcg ccttcttagc ttaaaacaaa
     3241 gaaatataaa ttaactttct ttcattctaa tatctgcctg tataaatgtc aaacaacaca
     3301 tttcttgtca taacctcctc tttttgaagt aagtacaatc tcccagacgc aataatatca
     3361 atttcctaaa accatttaag taacttttat atttacataa tatttacaat cataaactat
     3421 attgttattc atttatacat cataaacact ccctcctaat cattattata cttaaaataa
     3481 tgttcattta taataatata tctaacgcac tttaattgaa aaaactaaaa ctttgccttt
     3541 cattcccata tataaataag tatacttttt gatgctctat tacaaatcaa ctaaattaat
     3601 tgatataaat tatcattttc tgttgaaaat accattcgca catgctatac ttaattgcat
     3661 aaatggtaat aaatgtcata tgattttaca aatctattaa taacaattat tacttttact
     3721 gtattttaaa gtttttggac taaatatcaa gaactttcta aaatacaaag tacaaaaaat
     3781 ttatataagt tataaattta tatgaactag ataagctcaa acaatatgtt atgtataaac
     3841 tgtgtagtat tcggttataa atttatataa attatataag ctagataaag tattacctca
     3901 actatataag ctagatatat aaattgctta tataagctag atacataaat tgcttatata
     3961 agctagataa gaattatttc aactatataa actacatata taaattacat acataagcta
     4021 gatatataaa ctgcatatat aagctatata agaattattt caaccatata aactacacat
     4081 gtaaaattca tataaattta ctaatataaa tcaaataaaa ttactaaact ctctaaacta
     4141 attttgtaaa acatactcta tttttaacat ttgttacctt atacataagt acaaacaaaa
     4201 tctaactaaa tgaaaggata atgtaaaatg aatattttaa aaatgaaaaa aacagggata
     4261 tttttaggag gagtactttt tggtactgta ggtgttaaga tactttcaag tgattgtgtt
     4321 aaaaaaactt gcactcgtgc aacagcagga gcattgaaag ttaaagattc tgttcttgaa
     4381 acaactacga aaattcaaga aaatgtagaa gatattttag ctgaagcaaa agaaatcaac
     4441 gagcaagaag acttatctac atctaaggta aacgttgtac aagatactct taaatgcgaa
     4501 caagaagttc ttaaaactga aactataaaa actgtagaag atactaattc taatagcatt
     4561 aaatgtgaag aaatcaaaga taacgtaatt gaagaaaaaa atagtacttg tacaacagaa
     4621 taaatttaat gattattatg attaataata tcaggaggaa gtaaattgaa tttttatata
     4681 aaacacgaaa taagaggtcg tatacgcttt gacttacaac ttggcaaatt aaacaacaag
     4741 cagtccgata ctctcttata ttatttaact tctataaatg gtattaccaa agcaaaagtt
     4801 tacgatagaa ctggtgatgc agtcatttat tacactggtg aaagagaata cataataaga
     4861 gaaatcacta aattcagttt taacaatagc aaaatagaaa acctagttcc tgaacatacc
     4921 agcagggaat taaacaatta ttatagagac aaattagcaa ataaactgct agtcaaaagt
     4981 atctccaaaa tattttttcc aaagtcctta agattttttt taattggact tcattctata
     5041 aaatatttta aaaaaggtct ctcttccctg ctaaataaaa aaatagaagt ttctgtttta
     5101 gatgccactg ctataggtat ttctattttt agaaaagata tcaatacggc tggttcagtt
     5161 atgtttcttc ttggtatcgg tgaactatta gaagaatgga ctagaaaaaa atctatagac
     5221 gacttggctc aaagtatgtc actaaacatt gaaaaagtat ggttaagaaa aggtgacact
     5281 gaaatcttaa ttccaatttc agagataaag gaaggagatt tagtaagtgt aactatggga
     5341 aatatgattc cattggatgg agttatagta tcaggagaaa caatggtcaa tcaggcatca
     5401 cttacaggtg aatcccttgc cgttaacaaa aaagaaggct cttacatata tgctggaact
     5461 gtaatagagc aaggcaatat tgttatgtgc gtcaaggaaa aagctggtac tactagattc
     5521 cagaaaatag taactatgat tgaggaatct gaaaaactaa aatcttctgt agagagtaaa
     5581 tttgaacatc tagcagatac attagttcca tacagctttt taggaagtat attaacctat
     5641 gcaattacta gaaaccctat aaaatcactc tctatattaa tggtagattt ctcttgtgca
     5701 ttaaaactct ctatccctat ttcagtacta tcagccatga gagaatgtaa caataacaac
     5761 attactgtaa aaggtggaaa attccttgaa ggtgttgcat gtgcagatac tattgtattt
     5821 gataagacag gcacattaac aaaagctcaa ccaactgttt cagatataat tacattccaa
     5881 aattataata aggaggatat gttaagactt gcagcttgct tagaagaaca ctttccacac
     5941 tccatagcaa atgcagtagt ttacgaagct gaaaaacaag ggcttagtca caaagaaatg
     6001 catacagagg tagaatatat tattgctcat ggcatttcaa caaaagttga tgataaaaag
     6061 gttgtaatag gtagcaaaca ttttgtattt gaagatgaga agtgtactat cccagacggt
     6121 gaggaggata agtacaacaa tctatcagat gagtattcac acctatttat ggcaatatca
     6181 ggtaaattat cagcagttat ttgcataaat gaccctttac gaaaagaagc aaaatatgta
     6241 atttcaaatt taagagaatg cggaataaaa aagattgtga tgatgactgg agatagcgaa
     6301 aaaacagcta aatctatagc aagtaaagtt ggtgttgatg aatattattc agaagtctta
     6361 ccagaagaca aagccaattt tgttaaaagt gaaaaactca aaggtcgcaa agtaattatg
     6421 attggtgatg gaatcaatga ttctcctgct atttcagaat cagatgtcgg aattgcaatg
     6481 agtgaaggtg ctgaaatagc aagagagatt tcagatatta ccatatcagc agataatttg
     6541 aataatttaa tcgttttaaa gcagattagt aataagctta tgaaaagagt tgatttaagt
     6601 tctaagttta ttattggctt taatttaggt ctgattttac ttggtgtagg tggatttgtt
     6661 agaccaagta catctgcctt tttacacaac acatctactg tcggaataag tttaaacagt
     6721 atgacaaatt tattggaaaa caccaatact tataactacc attaatctcc attacataaa
     6781 acatatgata taaaaaattt ataattcatc tttttaaaag aatatgtctt aaaattagct
     6841 tttttctaat caaaattata taaagcatac tttacaaatt ttaaactcaa cttatttcta
     6901 ttttttacaa aaaaggagat gattcaaatt ttcattttga aacatcccct tttgtaaatt
     6961 tctttaaaat ttatatatta attatatttt caaccaataa ttgtattttc aaccaatctt
     7021 tcttcatagc attttaaatc attttgcttt agataattaa attcagactc tacataaata
     7081 ccacagccta ccaattcaat tgtcaaatta ggtatatttt cctttagtat actagtctgc
     7141 tcataaataa ttctttccaa taaatcaact tgtaatatag aaaaatgctt cataatctcc
     7201 ccatcactat ctatatcttt cttgatttca taccaactct ttgacaaaat tcccttcaaa
     7261 tcaatatacc ctagactcct caatatatct atactatcgc tccatgcata cttactaggt
     7321 ttatcaaagt atgattctag tttcttcttc tgctcatttg taagtgaatc ataagaaagc
     7381 ttcttattaa aatgtgcctt caaagaaatc ttagccatag aatatggact tctaccagag
     7441 tttaatcctg catttttgaa cttttcaatt cctttatctt ctactgaaga ccatattgag
     7501 tttattgaat ctgaatcatt gtatttaata tcaagaccat gtgtaatatt tatatagttt
     7561 tgtggaaata catattgata atagacatct tttttatcat catatagaga gaagtatcca
     7621 ctatcatata aatgaatatg cacctttact tttaaactat cctctaaaat atttcgtcta
     7681 aggttcggga tatttgcata catggttaca tattttatgg catcaaagtt tttatttaat
     7741 ggaacttcaa aactaatttc atctcctgtc aaaagctttt tcacttcttc ttcattacaa
     7801 tcatatagag aatatgtatt ttcaaatccc acacgtgatt tcccatctaa actgcctgtt
     7861 aatgctacct tcaaaatatg gttatctgtt ttttgccatt ggtaagcatc ttggtcaaga
     7921 aactctctaa tctgtctatt ttgctctttc gcctgattta taacctcgtt ttcatcttct
     7981 gtaatcccta catggcatct atttaagata tttactatat tgcttccaaa tgggaatgtc
     8041 catagttgaa aagctgctgc ctcaacctga cacaaatatc taacctgatt taattcaatt
     8101 tgatggataa gttttttctg atattcattc atctgattac cactatctga catttgataa
     8161 atcaattctc ccattgcaag attatctttt ttcttatcaa tcttatcagt aatgataatt
     8221 tgagtttcca tcatatcctg tactttttta aatattatag aaatctcctc tgagttttcc
     8281 atttttaagc ttccaaactt cgtgcttaat tcagttaaaa cttccttcat ctttaattta
     8341 cttatctcat tttcaaaaat atcttgattt ttaactgtac tatcgaattt ttctacaaat
     8401 ggtataacag attctttcaa agttttatct ttaaattcca ccatttgagt attcaattca
     8461 tctaatttct taatattatt ttctttatct ttaattgcct ctttaactac ctctatttta
     8521 ctctcaaaac tagattttac attaagacaa ttattaacac cactaatcgc agtatttata
     8581 agagaacaag cagaacttcc aatactcaat gcatttttaa ttttagatgt cttttgctgt
     8641 ctttcaatag cttgtttact tttctctagt gcagctaact tatctttttg ctgtttatta
     8701 ttgtaatttg cctccaacat acctatttct tttttctgga tttttgtgta aatttcctta
     8761 tactcgcttt cacttaaccc ctcttttgct ctttgaatat cgtcagcttt gtctgcatag
     8821 ttatcatcat ctatagactt aactataatc tccttaattt ggtcaaattt agttcttctt
     8881 tcttcatcaa cacctgccaa attataagtc attttatcta aagcattttt tctatcttca
     8941 cttagctgtt ctccaagtac aaatctcata ccatcagaat ccaatttaaa atctgaaaaa
     9001 tcaaccatcc tgttagtcca ttggtattcc tcttctttag ctgaaagatt ttccttcaaa
     9061 gattttgcac tgtcaaatgc ccctagacaa gcttgaccaa caccactaaa tgcagtcagt
     9121 aaactcaatg cgctttgtat tccagaaaga attaactgtg ttttcatttg ttcctcaagt
     9181 tttgcctgtt tcttttttag ttcctctatg tcttcttgtt catcttcttt catctttata
     9241 atttcttgaa tcaacaggtt catttttgca ttaacagaaa gcattttatt ctcaagttca
     9301 ttttttaaaa cctctatttg ttgatttcca gattcaattt gagttaggat actttcctta
     9361 tatgtattga aatatccttc ttttaactca tctttctttc cttgttccaa ttctcttaag
     9421 gtcttatcca gatagtctgt caaagaacga atatctgtaa cacgatttcc tccaatttca
     9481 ctttcttcaa tcatctgcac ataaaatgct gtcttctgct caagtgcaaa taaaatcata
     9541 ctccattcgc taaaattctg attttttcct acaaaagatt ctattctatc agactgcatc
     9601 aatttattta taaggtattc atagatttcc tttgcctctt tataaaattc tttctttata
     9661 ttcatgtcat ccatagaaag tactgcttta tttttcttct caaaactata gtacttctga
     9721 agtatttctc tatttaattg catctctatt ttctcaactg cataaaaaat ttcataaata
     9781 tgtgaaaatt ctaaaatatc agcttctaaa attcctactg cacacaacag tctaagattt
     9841 ctataacctt taattccaga aaaaccaaat ctctcgaaaa attcatagaa gaatgtatct
     9901 attgacaaag aatactcctg ctttaacaat tccttttctt ttagtgcatc tccagagtga
     9961 ccggcttttc cctctgctcc ggctgaacct gaatcttcac gtttgctaat tgtccagtcc
    10021 ttatgttctt cataaagagt atttaatttc tctccatcat ctgctgcttg cttctgattt
    10081 aaagcatgat taaacacata tctatcaaaa taatagtagt ttctatcact attatccatt
    10141 ggaggagagg tacctatata tcttattgtt tcttcatata tacctctaat tccaccaaga
    10201 cctcctgcac caccagctcc tccagagcct ccttttcctg ctaaaccatg atttccatct
    10261 tttttactta tagcactttt taataaagga gcttttccaa ggagataaac ctgtccagac
    10321 ttgcttccaa gacctgcatt tccaccatta cctcctgtgc ctccagttcc accttgtttt
    10381 cctttttctg cttttacaac atagtatttt gtacctttaa cattagcaaa ttcttttgac
    10441 tcttttttag aacctatttt agcatttgcc ccagcttctc cagttatccc tgtatctcct
    10501 ttttttccat cttgtccatt tccaccacgt ccaccatcta cgtaaagttt aagttctcct
    10561 cttttttcta tttctttaaa agataatatg atatcacctg aagtacctgc attattgcca
    10621 tttcttccat tcattccgtc gtaaatagaa ctttctccaa ccaatttttc tgcatcctta
    10681 ccagatacgt tgattgaata agtcccttct ggtatatgta atttttctgt aagtagtaca
    10741 atattcttac ctgagaagac ttctttctcc atattacagt caattttcaa ctcacaatct
    10801 ccatacatat atatagatga caaaatatta agttgataac cagatattat tttagtcaaa
    10861 tccgataaat tcactttagg tataggaata tatatagaaa gtacattttc tttctcatat
    10921 gattttatat atgtacaact ttgaatcttc tctatcctat actctgcatc ttcaagcttt
    10981 ttggcttctt cttccctata ttcttcttct gctatccaat atttgatatc ttttaatgta
    11041 tcttctaaag tattttttag caatgatttt aacaactcac ttagaccaac aatatgagtc
    11101 tcactacagt aactatttaa ctctaaaaga agacgtgttt cttctaatat attcactttc
    11161 tgtacgccat caccatatga aagttcttgt acaaactcca taagctcttt catattatta
    11221 gtcctactac ataaatctgt atacatctta tctaattgaa tctttttatc ttttagacta
    11281 tttatagaag atagaatgga aatagaatct tccatacctt ttttaacact ttctatataa
    11341 ggaactaaaa gtttttctag gtctttatag gttacatcaa cagctttttg tatttctatt
    11401 ttacaagaag ctgtaagcat caatcccaaa tctgtttttt caaatgtttc aggtagattt
    11461 ttcatttcat ccaacaactt ttctggctgt ttatctagtg gattacataa tttaatagat
    11521 acaccatctg ataatgctac atttaataag tacactaatt ccttacaacc gtcgtaatct
    11581 ttctcttgac tatctaagtc ttcttttgct ttttccaata tatttcttat tttttcttct
    11641 gttacattac cgattagtgt tttaggtatt ttattaataa gaaagaagaa attttcttta
    11701 tactttgcat aatcaccaag catatctttc aaatttttgc ctgtttctat caataatgta
    11761 tgtctagagt ccagtaggga ttgatagtct aagataacta ccatcccttt cacctttgaa
    11821 gatgtggaaa tagtttccct tacaagcata gcaccagcaa tcatatactc tgctccacgg
    11881 ttgtcaaaaa aacctgggca atcacataat gaaaaatctc tatttgcaat atctacaact
    11941 tctggacata aagtcttaga atatacacca tttccaattt cgacagctga atcttttgca
    12001 gttatatata tttgtccagt ctcttcatcc gttttttctt ccatcacaca gccttttaaa
    12061 taatttatac aagtactctt tccaacgcct gaagcaccta ggaataacaa aattgacttt
    12121 tcttttaagt catattttct aagctcgtct cttccttctt ccaaaaggtt tacgatttca
    12181 ctataaagtt tatttgccat acatccatcc ctttctatat ttcggaacaa tagccttgat
    12241 atttttcttt aataagattc tcttctacct ttgaaacagc tgttgataac ttagtatttg
    12301 gagcattaaa tcctccattg tctcctctat ctccatattc agcgtattct cttgctggtc
    12361 ctgttttacc ttcattccag cttccaccac taaattctat atgatattta attatactat
    12421 ctaacttata atcccaactt gtaaaatatc ctgtatagta tacagtctta tctataccac
    12481 ttggagaaaa tgaaagtgta ttatatccgt gatagccacc acttcctcca tatccaccta
    12541 aacctggtct accatttgga cctgcttctc catctttcac tgttatatca caatgattaa
    12601 ttattctatt tttttgttcc tctgaatctg aaatgaagta aatattacct ttcttaccag
    12661 catttccacc tcttccagca tttccaccag ctccaccttt ttcacctttt ttgccacttg
    12721 tacctttttc aaaataccct ttaaacatct gtccgtggaa ataacttgta tatcctgcat
    12781 cagctccatt acttccagaa gctcctgttt caccattttc tccatctatt cctttagaac
    12841 catttcctcc atttgcaatc agtgttagac gataatttct aatttcttct ttaatatata
    12901 agtaaatatt acctgcatct ctaccatctt caccattttt cccatacata gaattatcta
    12961 caagttctcc gctctttcca cttacattaa tttttacatt actacctaca tcaagattgt
    13021 tagaagcaag aataatattt ttctctgatg caaagttcat tccaaggtta tcatccatat
    13081 aaatagtatc tttagcaaag aacataaaag tagaaacatt gtcatctact tttctatttt
    13141 tgaaatattc caatacactg ctaattacta agtttggaat taatgctctt atttcaatac
    13201 ctttcttatc attaatatta attgttttta ctataaatgg atttaaaatg tatttaccaa
    13261 taaattctct aattatctca ttaccttctt ctccatatgc acttaatctt gttatactgt
    13321 cttctaactc ttcattttgt gtaactgtaa catttgtcaa aattgaatta aaatcactta
    13381 cactatatac ataatccttt ttatttgcaa cttccattaa tttttctcta tttggtaatg
    13441 taaatacctg tttttgcact tcataagaaa ctaaaacttt ttctaaattc tttcttatta
    13501 taatgaagtt ttgttccatt atcttcttac ttgccttttc tataataaat tccatatgta
    13561 cagaaggtaa taaaatttcc ccagttccat gttctgtata tttacttaaa gcatcaatta
    13621 tatctaagtt tccttttatc ttattttgta atttcatgca actatcttgt aagaataatc
    13681 cactttgttt ggctaatacg ttgataaaac tctctatagt aagacttgtt gtactttcca
    13741 accagttttt aaatactttt acaacctcat catattttgc tatttcttga atcattgtta
    13801 tacttccaat aatattgata aaatcttctg tatacgcctt aacataatct tctaggctag
    13861 atacgatatt gttttgaatt tcttttatca acgtatctaa aactaatctt gcatctgcac
    13921 taattggata ttggaatgct gatgtcttat ctttaacttc atctaattct agtacttgct
    13981 ttaataagcc ttccctctca tctgatgcaa gtggcttaca aagatgaata ttttttccat
    14041 tatcttttag caattcttca aataaccaag ctttttcctc tatattactg tctaaatttt
    14101 cgcataattg ttcatatttt ctttttataa cattatatat ttgtttttct gttcttccta
    14161 agaaactttt tgaaatgaaa aaagttatac tacccttaaa attttcttta tgaagacaca
    14221 tatttaactg ttggaacagg tctagtactt ttgtaagtct agaatctaat atatctgctt
    14281 ctggtatcaa tacaattact gcacatatag atgcagaatt tttaaatact tcacctaatg
    14341 aaactgctgc actaatatca tgggctactc ccctattgtc gctaaaacct gcagtatcac
    14401 ataattttgc atccattcct tctattggtt ttctcatatt tacaatgttc gggtataaag
    14461 tttgagaaac agctcctgaa ccaatctctg ccacagggtc taagcaatca atatatgata
    14521 taccagtatc ttcatcttct acttccatca ttacacatcc tgcaagatag ttaattgcag
    14581 tacttttgcc acatcctgtc ccaccaagaa ttagaattaa attttgattt tcagccttat
    14641 ttattgctac ttttccttca tttaataaaa gtgttatttc ttctaaaagt tgattgttat
    14701 tttctggcat ttattttccc ccttggttat atattttatt taaataaagt ttaatccctt
    14761 aagtagattg caaagttttt agactatatt taaatttgtt ttttattttt tatttttaat
    14821 aaatttgcat tttcactaaa taattattct tctttgttta ttaaaataac tgtgttgtat
    14881 aatataatct ttatcaacac agttatttta tttaatgcgc ttattttatt gtattttatg
    14941 taatacagtt gttttttata ttatatgtcc attatttttc tatggttatt acatatagat
    15001 ttttaattat atttgtacca ttaatttaaa cattcctata ctactgctta agttttacac
    15061 ctataccagc ttgaatattt tatttttatt tgcttaaata gttcaactat actggtttaa
    15121 ataggttatc tttatttgct taaatagttc aactatactg gttaaatagg ttatctttat
    15181 ttgcttaaat agttcaacta tactgattta aataggttat ctttatttgc ttaaataagt
    15241 tcaactatac tgatttaaat aggtttcctt tagtttaaat aattcaccta tactggttta
    15301 aataggtttc ctttagttta aataattcac ctatacctac ttgaatagtt cacttttatt
    15361 tgcttaaatt ctataccaat actggcttaa acattatact accatgattg tattgtctat
    15421 cttcaactac aataaaaaca ggttctaaat ctttagtaga tgttattttt aattctaaaa
    15481 aaatattatc agttggtaat atttttacat cataattatt taaattatta ttcgtgatac
    15541 tattaaatac atcaccttga cgttcaaaaa aaacattatc aactattaca aaataaacat
    15601 cttcttttct actattttca acctctatag tattttgttt agttatattt agtctcatct
    15661 tccacctcct attaatatta cttaattata ttaatacttg taattgttgc attatgttca
    15721 tcacaaggaa gaggtttaaa aacaactatc tctgctaaat aattctgtgg aggatttgta
    15781 actgaagtag cattgcaaat aaatttaaaa tttgttcctg ctacaacttg agtagaaaca
    15841 ataagtggtt cataaccaac cccttctaac attcctacag cttcattaaa tacttttaaa
    15901 tcttcttctt cgacagctct gaattttgac catccaccaa caaaaagact catataaaaa
    15961 cctcctttat atatatattt atttatatat agtagtacca attttctcaa tattcaatat
    16021 tttctgtgta actggtcaat gaaaatgcat aactggataa aaatacttat aaaataactt
    16081 aactttacat ataaatagct tactttacat gtaataagat tattctacat gtaataagat
    16141 tattctacaa tatgaaatgc atcttgtttt gaatatattc taaatacaat aatggatatt
    16201 ttcaattatt ataattagaa aatatccatt acttaacttg ttttatttaa aatcacatag
    16261 agactttatt atttttttta ttgcatgaat agctatttca ggtatcataa ggtgatatac
    16321 cccctccttg aaaccacaaa aaacatgaca aactttacga atcttgtctt aaaaaactac
    16381 aggtacattc cttatttgtg gattggagta gccataacaa gcaaatttta caatttttat
    16441 gatgacaaaa atcaataaaa tcgcttagta tataacacga aatactactt gttactatta
    16501 agaatagtta gataggaagt atgcattata caaataaagg aatcaataaa gaaagttaaa
    16561 acatccctac aaattaaaaa tctgttacat ttttctatat ttaaacgtta tttacttaca
    16621 attggagaaa taacaggttt tccttctcgg tcaagtaaag gcgtaaatcc ggctgcattg
    16681 ccataatagt ggaatacata gttcacacct gtttctttat ccacccaaat ctccgttgca
    16741 tttattttcc cttgtgaata tactttttca aatctatctt ttcccatttt ataatcctcc
    16801 atatttttga ttggtctatg ccttcttcac ttaagtagaa aaaatacata acttattttt
    16861 ttaattctaa tatgagcacg taaaaatgtc aaacagctta tttaatttca taacttcatg
    16921 ttactgttcg taaccttgtc tgtttactta cactgcatta cagcaccatt ttatccttta
    16981 cattattgta ttgcacacag taaaaatgct tactttatgg gatttttcaa ctaaaaaaag
    17041 gattttatct ccacaaaaca tttactattt gttacaatat acctaatttt aatctcatat
    17101 caacatttaa tgaagatata acctttttca aatattgtaa agactcctta aaaatattag
    17161 aaaaagattc tgcaaatctc tcaacatctc ctttttttaa atacctaagt atctcaaaac
    17221 atacatcaaa acgtgttttc aaaatttcat tatattcttc atgaaaaaat ctctctaaat
    17281 agattccaaa tatcatatat tgttttaaat gcaaaaatgt ttcctgtaat acataaattt
    17341 gtgtataatc tactatctta caaagtaaaa caaaatctgg ataaacctta cactcttcac
    17401 aattattttg taagtacctt aaatgagttt ctattttatc tacagctatc aaatccattt
    17461 tatttcctag taaaagtact acatcctcaa gagtaatcgt aacaaactcc atagcatcca
    17521 aaagcaaact caacctttta gctccttctt ctgtatttat ataatcatca tcatattcag
    17581 aaaaaagcgt tacatatgtt cccttgccat taattgtttt agcaattccc aattcattta
    17641 gctcattata ggcacttcta actgttggaa gtgatacatt atatctatta attatcgaag
    17701 taattgatgg tagacaatct cctattttaa tattgccttc aaaaatttga caaattacat
    17761 ctgaaacaat taagtcatat aaataacatt tttccaacgt tacagaatta aaactacttg
    17821 tatctatact ttgtggagca cgattttcta ccattttaag tatggattct ctacaagctt
    17881 tatacatata tgttaatagt tttttaaatt caattagatt cccatccaat ccatataagt
    17941 aagtttttct tacctgctct atgtatttat tacgaaaaaa gtctatacaa caattatcca
    18001 agttatccaa gttatcgaaa ttataggcca attgtgaaat aatgacatca tgctgaataa
    18061 tctcacaaaa atggtgaatc aggaaattat ttgttttact tattagttta tttatatatt
    18121 ttagcctttg ttctttaaag actaaataat tagaactatt tttttccata caatcgataa
    18181 tctcgtttaa ttcttcaaag tctttagatg taaatctttt aatattgtac atagcaatag
    18241 aaggaaatat taaactaaaa atcttatata tttctgatat ttgtaaaatc ttctcatata
    18301 ataagtcaga ttctattgat tgtttactcg tactctggat taaaactggt ctagaaccag
    18361 cttttgtctc tataaagccc ttttctgaga gcatttttat tacagttttt atagtatttt
    18421 ttgataccat atattcttct gacatgcttt ttatagaatc aagcctatct ccaatagtaa
    18481 ttttaccagt ataaatttgt tgaattatat gctcaaaaat aattaaatat tgtggttttc
    18541 tcttcatatt tttcctcttc gataaattag atttattgtt ttttgtaagg tatatttact
    18601 tttatttaat agggcaaaat agttgcttat tttttcctca taatctataa ttaatataaa
    18661 ccatttatat atatggtcta agttttataa tactgtaatg cagtaaaata gcatgttgta
    18721 gctatactac agcaaattaa cataccattg tagtatatta attggaataa ttttaagtac
    18781 ttcaaaattc cagcaaaaca tatatgtgtt tttgatatta tgtatattcg ttaatagaat
    18841 agctatactt gttttacata ataaattata tgatacttgc tatatgtaat cactatattt
    18901 aatacacata actattatac ttgctatatg taattactat actttataca catagttatc
    18961 atatatgcta tatgtaatta ctatacttta tacacatagt tatcatatat actatatata
    19021 attactatac ttggacacat agttatcata catgctatat gtaattacta cacttggaca
    19081 cataactact atattt
//
LOCUS       DAYATN010000066        18999 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.066, whole genome shotgun sequence.
ACCESSION   DAYATN010000066 DAYATN010000000
VERSION     DAYATN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.066"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(294..443)
                     /locus_tag="ACGI8A_002203"
     CDS             complement(294..443)
                     /locus_tag="ACGI8A_002203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180199.1"
                     /translation="MEIVAVYYIAKFLLDLYKVKSHSKSEAKSGFIFNLTINKLIVKI
                     KYKKY"
     gene            696..884
                     /locus_tag="ACGI8A_002204"
     CDS             696..884
                     /locus_tag="ACGI8A_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180200.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            complement(1294..1878)
                     /locus_tag="ACGI8A_002205"
     CDS             complement(1294..1878)
                     /locus_tag="ACGI8A_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180201.1"
                     /translation="MKGEYNKEKEKVIKFNSNKDKSKINKYTDYYNTLNSTLMEKNKC
                     IGDNWFLATTSEEVGNMNEEEKRILETEKRILETEKRVHQNNKEVKESIDKQFNDIKG
                     IFKEYKNDLDKDRIELKNDLARERIELKSDLKEAINEHKKVTEKDVSEIKNSIKGIED
                     RIDSTNKWIIALCITTIIGIATMAITIGISIWPK"
     gene            complement(1881..2372)
                     /locus_tag="ACGI8A_002206"
     CDS             complement(1881..2372)
                     /locus_tag="ACGI8A_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180202.1"
                     /translation="MAYENGTNLVAYFPNCIKKYEKDNKIFEKTILLLNLYMQMPTWP
                     HCIHEFKAKANEILGEDPEFIGEYGIKLKDFYNEGENVNISQNFKNDLYTFSNSTYDI
                     LDNSYIQRINPIGLYSISSLARENDFILKLFRNDKKSLELELSKSEIQSIIEHLESMI
                     EEE"
     gene            complement(2622..3434)
                     /locus_tag="ACGI8A_002207"
     CDS             complement(2622..3434)
                     /locus_tag="ACGI8A_002207"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0180203.1"
                     /translation="MKICITVGHSILKSGACTSADGVINEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKNEEKSYKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSN
                     KGLEYATRICDKLGTVFKNRGAKLDKRLYILNSSKPTAVLIESFFCDNKEDYDKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVVGGVACEKIGSMTKEKFTMIKGNDRFDTLYKALEFINK"
     gene            complement(3434..3865)
                     /locus_tag="ACGI8A_002208"
     CDS             complement(3434..3865)
                     /locus_tag="ACGI8A_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFH0180204.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQNWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKKLKDRIGNLLDTMTDELNIKGGSK"
     gene            complement(3927..4109)
                     /locus_tag="ACGI8A_002209"
     CDS             complement(3927..4109)
                     /locus_tag="ACGI8A_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180205.1"
                     /translation="MNINNIVVRILAERILSGGLNPLKNREFELDDVTNTEYRKAVED
                     YIIEHSGVVEGTEPTK"
     gene            complement(4109..4402)
                     /locus_tag="ACGI8A_002210"
     CDS             complement(4109..4402)
                     /locus_tag="ACGI8A_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180206.1"
                     /translation="MNRANRIIYDQTGKILLQTGEATGDILEHDIITQLHCIDVPYGS
                     IDYEKYRIIGINIETKEPISEEIPVYVNEEEKRIQELENQLLIAENEKVGGIL"
     gene            complement(4423..5106)
                     /locus_tag="ACGI8A_002211"
     CDS             complement(4423..5106)
                     /locus_tag="ACGI8A_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180207.1"
                     /translation="MTTKLTDNASLGELMTALQSVQTDFQTGKNNLSSALGNPFVGTD
                     KLETTKTKIETLKNSLAVNLTNKNVSANETESIQSLINKVGNIKISNYAAGTTSVVKN
                     TSLYAKTFYLNSTSNPSYWISITNLNFLPNVFIAECEYTTSDKWNKYYVIATSYFPSS
                     TVILTDFVLNVSFNCEFSASTLNSGASKLYENNKRDVYITGGSIRVPAFTESSSTLSS
                     YKWYALKIQ"
     gene            complement(5120..5926)
                     /locus_tag="ACGI8A_002212"
     CDS             complement(5120..5926)
                     /locus_tag="ACGI8A_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFH0180208.1"
                     /translation="MATDKSYYTILTDIGKAKIANASLVGEKVDFAKIQLGDGGGNEY
                     NPTEEQTALKNVVWEGNVGNVKTDEAMANCLILESLIPANAGGFIVREIGYLDTEGNL
                     LAISKYRSAYKPKVEDGAVIDMKVKTIFVVSNVNNIELKIDPTIIFATLKDLQDLDTK
                     IDTTKTELTSNIEIAKTELNNKIGDTTLLETTDKTSLVGALNEVKTSVDSIETTAEKT
                     SIKDTDNLFESDNVEGALKELATKQNLLDTEVNGQREKGINIANNLIDMI"
     gene            complement(5942..6559)
                     /locus_tag="ACGI8A_002213"
     CDS             complement(5942..6559)
                     /locus_tag="ACGI8A_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFH0180209.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFNEVHTFVSSIKPCNMSLDYTRVLVSKDNG
                     MYFGATTVTGHEITIYPWSPSDIETQGEIKIMSGNGFGYQEITIY"
     gene            complement(6552..7601)
                     /locus_tag="ACGI8A_002214"
     CDS             complement(6552..7601)
                     /locus_tag="ACGI8A_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFH0180210.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEGIYKMKCETAGPIFELGQLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKIQDIGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            complement(7602..8021)
                     /locus_tag="ACGI8A_002215"
     CDS             complement(7602..8021)
                     /locus_tag="ACGI8A_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFH0180211.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLHIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNIKGKVHCKFMVHTKYGNIKAEKVVSV"
     gene            complement(8026..8286)
                     /locus_tag="ACGI8A_002216"
     CDS             complement(8026..8286)
                     /locus_tag="ACGI8A_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HFH0180212.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPMQIAFGTIESVNPLVVKIEQKA
                     SFEEFFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            complement(8300..10267)
                     /locus_tag="ACGI8A_002217"
     CDS             complement(8300..10267)
                     /locus_tag="ACGI8A_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFH0180213.1"
                     /translation="MSDDLVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGAPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYNNKTASELVKMLTKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFETVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLIPVFLDLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNANNKDEKKNGPAQNTTSKEDNDMINKLNKVFKGKLSNTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKGMSFGSVDEGIKRGISNLSRNYIHIG
                     RKTLESIRDKYAPLYDSPLNKDWVPGVGKFYKQITGNAYSSNSAGTGVGSNEEAEKNL
                     KDTTYQVQNSNNANTSTNNNSKVSKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAF
                     KRGAGINLKRVSADQSKDSRGKLLCNINDVKAGDLVFFKNEQGKVHHVGLYIGNDQYI
                     HAPQTGDVVKISSLSGRQKKKHDFARARRFF"
     gene            complement(10260..10955)
                     /locus_tag="ACGI8A_002218"
     CDS             complement(10260..10955)
                     /locus_tag="ACGI8A_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFH0180214.1"
                     /translation="MAYDFYLDGVQLPIAPGKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYKGAFKDVQFFLSKLELLKTDCKPFQFIVSRQLGNKVLFN
                     TNIKVSLEEYNIVEDAENGSDTKVAIKLKQYRDYSTKKLVPATPKTTSDAGRPNVKIE
                     KKRVDSVNEPNTKTYTVKAEDSLWAICQKQLGNGSLYKKVYELNKSMMDKANKGKNLS
                     KYTIYKGQVLRLV"
     gene            complement(10967..13450)
                     /locus_tag="ACGI8A_002219"
     CDS             complement(10967..13450)
                     /locus_tag="ACGI8A_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFH0180215.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQRELRNPLD
                     EGGIQASVKIFDRMAPALKNMNTSINATAKSLERLQQKLNNPINAGNIQASQQNLKNI
                     ESILTRIEQKIGRNTNEQENFNNKIRKGSEEGSLLVSKLKSLAGVYIGIRGIESITKA
                     ADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAAQVAKLGILAGDAFG
                     SSAEVVKFTELMNKAFVIGGTSASEASAAMYQLTQAMGAGKLQGDEFRSIMENAPLLA
                     TKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFASMPITFSQALTMMK
                     NDAYMIFGQTLGKISGALQSVRFSEIVVSMRNVMIAISSNIYDTLNIIKNILNSDFFS
                     DFVQGITLGAVLIIQGLGGITNAALSVVNVFAQNWNIIEPIVYGVVAAIAIYSLALAG
                     MWLWEKLVAASKFAMMFAQMLLNTKTLAGYAYLIKYAAGQWIANEAMLACPIFWIVAG
                     ILAFIVVVFVAVAAVNKFAGTNLTALGVIVGAVFAAVAFIQNIMIWLFNRCVDVNEGI
                     TNGWNQCVFFMKQAIAKGVIFIIEKMASLNDSVNNAGNALGKAFVTGANIAIRGVNKL
                     IDLLNKIPGINIGKVGEATFTPVKADNSYIKQQINSLNRWVGDAPEKIKLERMQYKDI
                     GAEFQKGNALGTKWQNAISNKLKDTFDINKMLEDAKDKLGLKDLWDKNNPLNNLGGFG
                     GDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQEVINRFTGVNIKIDMNNTNNI
                     SKDADVDGIVNILTEKLNDAMVVSAEGVV"
     gene            complement(13519..14280)
                     /locus_tag="ACGI8A_002220"
     CDS             complement(13519..14280)
                     /locus_tag="ACGI8A_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFH0180216.1"
                     /translation="MGLFRKSKEPCCICGKEKTDQKIKDGSVCIECLMNYMEYKDKQF
                     TFKDGLPTKSEIKSVLDSKKQNDFLVEKFNVTKEINSFIKFDETNKLIYIETKRKNGK
                     IKKNVYSFEDITGFELLEDGETVTKGGIGSALAGGVLFGGTGAIVGSVVGKKKTKKII
                     ENLQIKLTLKAISEPVAYINLISGKTKTNSIGYERAYEDAQEILSVLSIILKDTENNS
                     NNSNADEILKYKNLLDLGAITEEEFNIKKKELLNL"
     repeat_region   14406..14633
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=14471..14501
                     /rpt_unit_seq="atttacataccacatagttaatataaaataa"
     gene            complement(14911..15069)
                     /locus_tag="ACGI8A_002221"
     CDS             complement(14911..15069)
                     /locus_tag="ACGI8A_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180217.1"
                     /translation="MDNFLQGVLASLVASLIVYLTSKLFKKVKSHSNTTKSDFDFKLT
                     IKFKRNKH"
     gene            complement(15392..15832)
                     /locus_tag="ACGI8A_002222"
     CDS             complement(15392..15832)
                     /locus_tag="ACGI8A_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0180218.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKPIEWELKAIDSD
                     RDRQLRKNSAVRVPVIGKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            complement(15834..16091)
                     /locus_tag="ACGI8A_002223"
     CDS             complement(15834..16091)
                     /locus_tag="ACGI8A_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180219.1"
                     /translation="MVKIKDKYEIKDSISFDYSKKRPIEECVGEMYRKAGLFLVDIAD
                     KLALDTIEGSSLKPITIKIKLDEDGIATIEKETKYLIMEVE"
     gene            complement(16144..16620)
                     /locus_tag="ACGI8A_002224"
     CDS             complement(16144..16620)
                     /locus_tag="ACGI8A_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFH0180220.1"
                     /translation="MSKNITMNVRDAISGSKAECFVTIKGKRYNFMQAINLEAKMEKN
                     KSEMPILGSITKGNKSTGSKFSGSATFYYNTSIFRELLYEYKETGEDIYFDMQITNED
                     PTSSVGRQTIILEDCNLDSGIIAKFDADGEYLDEDVDFTFENWRIVEKFNIANGME"
     gene            complement(16639..17949)
                     /locus_tag="ACGI8A_002225"
     CDS             complement(16639..17949)
                     /locus_tag="ACGI8A_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFH0180221.1"
                     /translation="MALGGGTFVTQNKILPGSYINFISAKRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTAEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKNDADYEGVVSVENKIKDTGLLESSLVYWTTGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSIRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMGKLYMTVSVS"
     gene            complement(17950..18135)
                     /locus_tag="ACGI8A_002226"
     CDS             complement(17950..18135)
                     /locus_tag="ACGI8A_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180222.1"
                     /translation="MIILNKTLSKEDDYKFTKEQIVNSKKYVNRKDLLNAILKENELY
                     SFSEVEEIINKFMKGVS"
     gene            complement(18119..18556)
                     /locus_tag="ACGI8A_002227"
     CDS             complement(18119..18556)
                     /locus_tag="ACGI8A_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFH0180223.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEAKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            complement(18549..18974)
                     /locus_tag="ACGI8A_002228"
     CDS             complement(18549..18974)
                     /locus_tag="ACGI8A_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFH0180224.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
BASE COUNT         5714 a         3557 c         2046 g         7682 t
ORIGIN      
        1 cataaatata tatgaactaa gaattgtaaa atgagttata agttatatca tccagttatc
       61 aaaaacatat actctttttg tttttgtgaa agttttttag gtggctaata atatatatac
      121 ttagagataa aataaaatat atttctaata ttcatagttt tattttttct caaaatgcga
      181 tataataaag atgtaaattc gtatcaaaaa gagataagag aactgcaatt ctcctacctc
      241 tttagaaata aggaactata atctattgtc gagagtgtag tttccgaaaa aatttagtac
      301 tttttatatt taatttttac tattagttta ttaatagtta aattaaatat aaagccactc
      361 ttagcttccg actttgagtg gctttttact ttgtataaat caagtaaaaa ttttgctatg
      421 taataaactg ctactatttc cacacttccc acctcctttc attagaaagt aggttagagc
      481 ataatgtgga aactgccact cttagatatt tagttcctta catataaatt ataacataat
      541 tttacaatta tcaaatatcc attctccata tttttttatt tatacaatat attctatatt
      601 gcaataaaaa acattgtttt aaatatatat acataggtat atactaaata tgttaaactt
      661 aattatggaa aatataaaat gaaaggaata aaaatatgac taagactata ttatgtgatt
      721 actgtaataa aggaataaat aaagatgata ataagtatat tacttttcat aagaaaagtc
      781 atatgaaaac taacatttgt attaattgtg cattaaattt gatagataaa gataaattaa
      841 atgaaaatat tataaataat caacatgatt attcaaagaa atgaaaaagc actctccata
      901 atgaagaatg ctctatataa taatgtttga cttagtaaag atgtgttggg gttacatatt
      961 tactttttta ttatatcata taactttgtg tatgaaaaag aatttaaatc aattttaagg
     1021 tgtgttgagt aatgttcttg atagtttata tgttgatgaa tttcaaaaaa ataagcactc
     1081 ttataaaaag agtacttttg gtatatattc aagcatttat ctaatacaat tatagcatgt
     1141 cttatgtttt agtatgataa ttttcgttcg ttttattatt acaggttata ctatagtttt
     1201 atgtatcctg ttaatatttt ttactatttt ttcaatgttt tgtttaagta taaaaagaga
     1261 taactatttt aattctagtt acctcttttt gtgttacttt ggccatatag atatacctat
     1321 tgtaatagcc attgtagcaa ttcctattat agtagttata cataatgcaa taatccattt
     1381 attagttgaa tcaattctat cttctatacc ttttatacta ttctttattt cacttacatc
     1441 cttttctgtc acctttttat gttcgtttat agcttctttt aaatcgcttt ttaactcaat
     1501 tctttctcta gctaaatcat tttttagttc tattctatct ttgtctaaat cattcttgta
     1561 ttctttaaaa atacccttta tgtcattaaa ttgcttatct atactttctt ttacttcctt
     1621 attattttga tgtactcttt tttcagtctc taatattctt ttttcagttt ctagtattct
     1681 tttttcttct tcattcatat tacctacctc ctctgaagta gtagctaaaa accaattatc
     1741 tccaatacat ttatttttct ccatcaaagt actatttaaa gtattataat agtctgtata
     1801 tttgttgatt ttacttttat ccttgttgga attaaacttt attacttttt ctttttcttt
     1861 attatattca cccttcataa ctattcttcc tctatcatag actctaaatg ttcaattatt
     1921 gattgtattt ctgatttaga tagttctaat tccaaagatt ttttatcatt tctaaatagt
     1981 ttcaaaataa aatcattttc tctagccaaa gaagatatag aatataagcc aattggattg
     2041 attctctgaa tatatgagtt atctaatata tcatatgtac tatttgaaaa agtatacaaa
     2101 tcatttttaa agttttggct tatatttaca ttttcacctt cattataaaa gtctttcagt
     2161 ttaattccat actcacctat aaattctggg tcttctccca aaatttcatt tgctttagct
     2221 ttaaattcat gaatacaatg tggccaagtt ggcatctgca tatataaatt aagtaatagt
     2281 attgtctttt caaatatttt gttatccttt tcgtattttt ttatacagtt aggaaaatac
     2341 gctaccaaat tagtcccatt ttcataagcc atcttaactc cccctaaaat aatattattt
     2401 aatactattc tacattataa ttactttcat tgcactatta ttttttattc ctcctcctac
     2461 cacttattgc tatatactca actgctcttc atatacctga ttttttaaag tttgtagatg
     2521 acttatggtt atatttaata tatcattttt tattttataa attgttgtga tactgtcata
     2581 aaattgtagt caaaatgtaa aatatattaa ctctattgat tttatttatt tataaactcc
     2641 agtgctttat aaagtgtatc aaatctatca ttacccttta tcatagtaaa tttttcttta
     2701 gtcatagaac caatcttctc acatgctaca ccgcctacaa cataaagatt ttgcgtctga
     2761 cctggcacgt aatcttttat atcacatatc agtattttcc catcattata accccaacca
     2821 actacagttg cagagatttt gtcaacttct ccatcataaa caattgtatg tttgtacatc
     2881 tgtttaactc cctcattatt tatattttta tttaatacac cttctacaat taacttagca
     2941 ataccttcat gacctagttt cttagcttta tcataatctt ctttattatc acagaagaaa
     3001 ctttcaatta atactgctgt aggctttgaa ctatttaaga tatataatct tttatctaat
     3061 ttagcacctc tatttttaaa tactgtacct agtttatcac atattctagt tgcatactct
     3121 aagcctttat tactataata taggacttct gaacctttac cttgaccgtt acttgcattt
     3181 aaatgtaact ctataagtaa atcatatcct ccactattaa ctctaggtat tttataagac
     3241 ttttcttcat tcttagtttt aaactgcttt tctgggcata ttattacatc taccttatgc
     3301 ccttcttttc taaatgtatc tgctaatact ggtgcaagag atttgttgta ttgatactcg
     3361 ttaattactc catcagcaga agtgcatgct ccacttttta aaatactgtg tcctactgta
     3421 atacatattt tcattattta ctacctcctt taatatttaa ctcatctgtc atagtatcta
     3481 ataagttacc tattctatct tttaattttt taggcactgg taacccacac aaatacatgt
     3541 tttttaatat acttacactt tcatatagaa taaataaaat agagaaaaat tcagatattc
     3601 caagatgatt taatcgtaaa aaatcaaccc aattctgcgg taacataaat aaaaaattaa
     3661 actttgtaag tatgtcaact actgctagaa aaaatataca tgctatcatt gcaacttttc
     3721 ttattcctcc atttattcca aaagagctat taaactgatg tgtttttatt gcccttaagc
     3781 aacctagtaa tgtatcaaat gctattgcta atattactaa ttttataaat atatttgttg
     3841 ctaaaaaaac tattgttacg ttcatatttc ctccttattt tgcattaaaa taagacttag
     3901 aattatctaa gcctataaaa aagaacctat tttgtaggtt ctgttccttc tactactcca
     3961 ctatgctcta taatataatc ctctactgct tttctatact ctgtgttagt tacgtcatct
     4021 aattcaaatt ctcgattttt tagagggttt aaacctccac ttaaaatcct ctctgctaat
     4081 attcttacta caatattatt tatgttcatt ataaaattcc tcctactttt tcgttttctg
     4141 caattaataa ttgattttct aattcttgta ttcttttttc ttcttcattt acatatactg
     4201 gtatttcttc tgaaattggt tcttttgttt ctatatttat acctattatt ctatattttt
     4261 catagtctat acttccatat ggaacatcaa tacaatgcaa ctgtgttatt atatcatgct
     4321 ccaatatatc ccctgttgct tctcctgttt gcaagagtat tttacctgtt tggtcataaa
     4381 ttattctatt tgctctattc attttatcac ctcattttta atttattgta ttttaagagc
     4441 ataccattta taagaagata gagtagaact agactctgta aaggctggta ctctaatact
     4501 tcctccagta atgtaaacat ctcttttatt attttcatat aactttgaag caccactgtt
     4561 tagagtacta gcactaaact cacaattaaa tgatacattt aatacaaaat cagttagtat
     4621 aactgtggat gaaggaaaat aagaagtggc aataacataa tatttattcc atttatcact
     4681 tgttgtgtat tcacactcag caataaaaac attaggaaga aagtttagat tagtgatact
     4741 aatccaatag ctaggattac ttgtgctgtt taagtagaat gttttggcat ataaactagt
     4801 attttttaca acagatgttg ttccagcagc ataattagat atttttatat ttccaacttt
     4861 atttattaga ctttgtatgc tctcagtttc atttgcagaa acatttttat ttgttaaatt
     4921 tactgctaaa gaattcttta atgtttctat ttttgtttta gttgtctcta atttatctgt
     4981 accaacaaaa ggattaccta atgcactaga taggttattt ttaccagttt gaaaatcagt
     5041 ttgtacactt tgcaatgctg tcataagctc ccctaaacta gcattatcag ttaattttgt
     5101 tgtcatattt tcacctcact tatatcatat ctattagatt atttgctata ttaattcctt
     5161 tttccctttg tccatttact tctgtatcta acaagttttg ttttgttgct aattctttta
     5221 atgctccttc tacattgtca ctttcaaata agttatctgt atcttttata cttgttttct
     5281 ctgctgttgt ttctatacta tctacactag tttttacctc atttaatgca ccaacaagac
     5341 tagttttatc tgttgtttca agtagagtag tatcacctat tttgttgttt agctctgttt
     5401 tagcaatttc tatgttgctt gttaattctg tcttagttgt atcaatttta gtgtccaggt
     5461 cctgcaaatc ttttaatgtg gcaaagatta ttgttgggtc tatcttaagt tctatattat
     5521 ttacattaga tacaacaaag atagttttta ccttcatgtc aattactgct ccatcttcca
     5581 ctttaggttt atatgctgac ctatactttg atatagcaag taaattaccc tctgtatcta
     5641 aataacctat ctctctaact ataaatcctc cagcatttgc agggataaga ctctctaaaa
     5701 ttagacaatt tgccatagct tcatcagttt taacatttcc cacattgcct tcccaaacta
     5761 cattcttaag tgctgtctgt tcttctgtag ggttatactc atttcctcca ccatcaccta
     5821 gttgaatttt agcaaagtca accttttcac ctactaaact tgcatttgca atcttcgctt
     5881 ttcctatgtc tgttaatatt gtgtagtaac ttttatctgt agccaaacta ccacctccta
     5941 tctaataaat tgtaatctct tggtatccaa atccatttcc actcataatc ttaatctctc
     6001 cttgtgtttc aatatcacta ggtgaccaag ggtaaattgt aatctcatgc cctgtgactg
     6061 ttgttgcacc aaaatacatg ccattgtctt tactaactaa cactctagta taatcaagac
     6121 tcatgttgca aggtttgatg ctacttacaa atgtatgaac ctcattgaac caatcttgat
     6181 ttctagcatc actctcaagg tgtatgttat aagtagcatt attaatagtt aattcataat
     6241 tgccttctcc aactacatta tctagccagt tccttaaaaa tctctctgag taaggtaatt
     6301 tacttatata tttactaaaa atcctaaacc ttctatcttc taaactctca ttacttttag
     6361 gagttataga cattatcttt tcccatcttt ttataccact tatagttagg tcctctaaaa
     6421 actggtcatt tgataggtcc tttaatttat catgtagtgt ttttatttct ttattttcta
     6481 cattaaatac ttttatatat tcttctttat cttgtagaat ttgtggcaag taatttatta
     6541 gattaatctc tttatccaac tacctcacct ctcactacta tactgttact atctattgtt
     6601 agattagatt taacatcatt tatcattgtg tttgtaatat ctaacactcc atctatacta
     6661 agtaatctag tttcaatttg agatatacgg actattaagt tttcttcatc ttcccaactc
     6721 atgttaagtt catttaaata gtcgtctatt gcttcttctg caattgattt tatattctcc
     6781 catgtatatc cactcttata tgttatttct gctgatatat ttatagttgt acttgtaact
     6841 ccttcaactg tgactctatg acctattggt gctaatccta agccttgtcc ttgatgtcca
     6901 attgggtcaa tttcttcttg cactaaatta actaaatcct ctgatggtac tttgaaatta
     6961 gaattaatta ttactaactt aacagtacct ccaccgttcc aaactggata aactttaact
     7021 cctccaatat cttgtatttt gttaacttca tccttatagt tttgtatatt tccaccgaaa
     7081 ctttgtgaat ttaaactatc atagtacctt tgtcttaaac tgtcctcgct ctcttcatct
     7141 tctccattta tcagtatttc tgttaactct gctgtttcaa gaccgtctat atattcaatt
     7201 ggaatcaact gaccaagttc aaaaataggt ccagcagttt cacatttcat cttatatata
     7261 ccttcagata ttctctcaat tgctacatag ttatactctc ctaaattaaa cctagaatca
     7321 agtggaatat ctatattgaa aacgccttta gcaatcgtat tagtggctgg caaaggtgta
     7381 attcctctct ctttacatct cttctctaaa tagtaataac tagcagtatc tacaaatgtt
     7441 tggtctagta attcatccat tgcaatgtat gtttctgtaa gttctatagc aactggtgcc
     7501 aaggcattat atattataga accttctctt ttatcaaaag tatctggtac actatctaac
     7561 attcttttaa ttatattttc aaatgtcatt aactcaaaca attatacact caccaccttc
     7621 tctgctttta tatttccata tttggtatgg accataaatt tacaatggac ctttcctttt
     7681 atattttgaa actcaaaatt atctacattt tcaaccctat catcttgaat tagtgcttct
     7741 ttgattcgtc tttcaagttc gggtattaca aaggatatag gctctccaat aaggtcgttc
     7801 aactcgactc cataatttct actgtatatt aaatgctcat acctttcagt atttaaaatc
     7861 aaaaagatgg tttgttttaa tgcttccaca tcatcacaaa taccatctac tctgtttttt
     7921 tctatatgaa gtttaaatgt cttacttggt tcttgcctta catcaaaatt tataattgat
     7981 acatcttcaa tatcatagtc aatgttatct gttggtaaca ctttatcaca tcctatctaa
     8041 aatcaagtat tgttgtcctc cttgcatacg aattaaaacc aatttatccc ctattttttt
     8101 atctgtatat cttttgaatg tatctgtttg tattagaaaa aattcttcaa aagatgcttt
     8161 ttgctctatc ttaactacta aaggattaac actttctata gttccaaatg caatttgcat
     8221 tggattactt gtttctactg catccattgc tgctttcttg attatttgca ataattcttg
     8281 gctcatttta tcacctcgct taaaagaatc ttctagctct tgcaaaatca tgctttttct
     8341 tttgtctacc acttaaacta cttattttta ctacgtcacc agtttgtgga gcatgaatat
     8401 attggtcatt tcctatataa agtccaacat gatgtacttt tccttgttcg tttttaaaga
     8461 atactaaatc acctgctttt acatcattta tgttgcataa tagtttccct ctactatctt
     8521 ttgattggtc tgctgaaact cttttgagat ttattcctgc acctctttta aatgcccaca
     8581 ccataagacc actacagtca aaactctttg gaccattacc accccatttg taaggcttgc
     8641 caagttgatt ttttgcttct tgaataactt tacttacttt actattattg tttgttgatg
     8701 tattagcatt gttactattt tgaacttgat aagttgtatc ttttaaattc ttttctgctt
     8761 cttcattgct cccaactcct gtacctgcac tattagaact atatgcattt cctgttattt
     8821 gtttataaaa tttgcctact cctggtaccc aatctttatt aagaggacta tcataaagtg
     8881 gagcatattt atctcttata ctttctaatg ttttccttcc tatatgaata taatttcttg
     8941 ataaattact tatacctctt tttattcctt catctacaga gccaaaagac attcctttca
     9001 ttccaaagaa attattttta gtatttgcaa tatttgaagt ccctctagct gattcgtgca
     9061 tagatatagc agccatgagt gctggattaa ctttataagc atttgaatat ttaacaaata
     9121 tatttcctgt atttgataat ttacctttaa atactttatt taatttattt atcatgtcat
     9181 tatcttcttt gcttgtagta ttttgtgcag gaccattttt cttttcatct ttattatttg
     9241 catttccact actgtagctt gaagaagaat aagaagcaaa ttcatctcca tcaacaagag
     9301 ttaaatccat gaaatgtgaa ttattttcaa atgtatgttt tactttctca actaacatat
     9361 aattttgtaa ctcaatatct cctaaatcta aaaaaacagg tattaaacaa cctgctctta
     9421 ctctaatatc tcctaacaca ttttttaaac ttaatgactt agttttctta ttatataatt
     9481 ttaaaagtat atcacacttt tgttttatct ctgcttcact catgttttta tcaactgtct
     9541 caaacatttg aagtattccc caacttctca tatgtgctga gtcttgagca atatacacat
     9601 ctctttttcc tgactcctcg ttgtctctga cgagtttaat ttttgtataa gtatcactat
     9661 ctatacttga attatagtca aaatcctcta tgacatcatt attcataaca gtatctaatt
     9721 tcattgatgc aacattcttt aatgttattc ttccaaaatc atcatagagt acatacattt
     9781 cctttttctc tcttagagta tcatctagtg ctgttagtat catgtcaaat aaagttttat
     9841 tttcttcaac tctcgatatt ttatattttg tgtcttctat gacattgtat tttaagttaa
     9901 aatctttagt tagcatcttt acaagttcac ttgctgtttt attattatac acataagtat
     9961 ctttattctt aaaatatctc aactggtcgt aagcaacaat tttaatatga ttttctttat
    10021 ctcttttctt ttgaaatata tatccataaa atatacctat tcccttataa tacagcctta
    10081 cagagttacc ttcacaaaac tctaatatat catccatgac tattgtaaat tctaatttac
    10141 ttggcgctcc tcgtctttct atctcccatg tgattccatc taaaacgaca ggctcataga
    10201 aatcttccca atgtgctatg actaatctta catctctatc atttgctaac actaaatcat
    10261 cagacaagcc tcaacacctg ccctttgtaa atagtatatt tacttaaatt cttcccctta
    10321 tttgccttat ccatcattga tttatttagt tcatatactt tcttatatag tgaaccattt
    10381 cctaattgct tttgacaaat tgcccaaaga gaatcctctg cttttactgt atatgtttta
    10441 gtgtttggtt cattaactga atcaacacgt tttttctcta tctttacgtt tggtctacca
    10501 gcatcactag tcgttttagg tgtagcagga actaattttt tagttgagta atctctatat
    10561 tgctttaact ttattgcaac ttttgtatct gagccattct ctgcatcttc tactatgtta
    10621 tattcctcta atgatacttt aatattagtg ttaaatagta ctttattccc aagttgtcga
    10681 gatacaataa attgaaatgg cttacaatct gtctttagta gttctagttt acttaaaaag
    10741 aattgaacat ctttaaaagc acctttgtaa aacggtaatt tattatgtgt aaattctgct
    10801 tcaaaactta tttcagatag cccttccttt tttaatatgt ttacttctcc agtatttatc
    10861 aaatcaactg tcttattctt gtttgttact ttaatttcaa gtttaccagg tgcgattggt
    10921 aattgtactc catctaaata aaagtcataa gccatttata tccctcctaa actactccct
    10981 cagcagatac aaccatagcg tcatttaatt tttcagttaa aatattgact ataccatcca
    11041 catctgcatc tttacttatg ttgtttgtat tgttcatgtc aattttaatg tttactcctg
    11101 tgaatcggtt tattacctcc tgctctgcaa tatctctaag atattttaaa tcttcttgac
    11161 ttttatccat tgttttagcc atttttgcag tgtttcctgc cgtgtccttt acattttttc
    11221 ctaaatctcc accaaatcct ccaagattat taagtggatt gtttttatcc cacaaatctt
    11281 ttaatcctag cttgtctttt gcatcttcta gcatcttgtt aatgtcaaaa gtatctttta
    11341 atttattaga tatagcattt tgccatttag ttccaagtgc atttcctttt tgaaattctg
    11401 ctccaatatc tttatattgc atcctttcca attttatttt ttctggtgca tctcctaccc
    11461 atctgtttaa actgttaatc tgttgcttaa tgtaactgtt atccgcctta acaggtgtaa
    11521 atgttgcctc tcctactttc ccaatgttaa tcccagggat tttatttagc aaatctatta
    11581 atttatttac acctcgtatt gctatattag ctccagtaac aaaagcctta ccaagtgcat
    11641 tacctgcatt attcacagag tcattcaatg atgccatttt ctcaattata aagattacac
    11701 cttttgcaat tgcttgtttc ataaaaaaaa cacactgatt ccaaccattt gtaatgcctt
    11761 catttacatc tacacatcta ttaaacagcc atatcattat gttttgtata aatgctactg
    11821 ctgcaaatac tgcacccaca attactccta aggcggttaa attcgttcct gcgaatttgt
    11881 ttactgctgc tactgcaaca aaaactacta ctataaatgc tagaatgcct gcaactatcc
    11941 aaaaaatcgg gcatgctaac atagcttcat ttgctatcca ttgtccagca gcatatttta
    12001 taagatacgc atatccagct aaagtttttg tatttaaaag catctgagca aacatcatag
    12061 caaatttaga tgctgcaact aatttttccc ataaccacat tcctgccaaa gctaaagaat
    12121 aaattgcaat agctgcgaca actccataaa caatcggctc tattatgttc cagttctgtg
    12181 caaaaacatt aacaacgcta agtgcagcat tagtaattcc tccaagccct tgtattatta
    12241 atactgctcc taatgtaatt ccttgaacaa aatctgagaa aaaatcacta tttaatatat
    12301 tttttataat atttaatgta tcataaatat ttgaagatat tgcaatcatt acattccgca
    12361 tagacacaac aatctcacta aatctaacgc tttgcaaagc tccacttatt tttcctaatg
    12421 tctgaccaaa tatcatataa gcatcatttt tcatcattgt gagagcttga gaaaaagtga
    12481 ttggcatact tgcaaatttc ttttctatct catcagaagc tttaaacagt gcatttctta
    12541 taacatctgc tgttattgct ccactacttg ataattcctt caattggtct ttagttttcc
    12601 ccattgcatc agctatttta gtagctagaa gtggagcatt ttccatgata gaacgaaact
    12661 catcaccttg aagtttccca gcacccatag cttgagtaag ctgatacatt gctgcactag
    12721 cttcacttgc tgatgtccct ccaattacaa aagctttatt cataagttct gtaaacttta
    12781 ccacctctgc tgaacttcca aaagcatctc ctgctaatat cccaagttta gcaacctgtg
    12841 ctgctgtatc tgcataacta gctcttgcac tttgagcaga caaataaatc atcttattca
    12901 gttgctctgt tgtctgtaag ccatcattca ttagatttaa acgtgctttt gtactggcaa
    12961 ttgtatctgc tgcttttgta atactttcta tacctcttat tccaatatat acacccgcta
    13021 agcttttaag tttagatact aatagagaac cctcttcact tccttttctt attttattat
    13081 taaagttttc ttgttcattt gtatttcttc caattttctg ttctatcctc gttaaaatac
    13141 tttcaatatt ttttaagttt tgttgtgaag cttgtatatt acctgcattt ataggattgt
    13201 tcaatttttg ttgtaatctc tctaaacttt tagctgttgc attaatagat gtattcatat
    13261 ttttaagtgc aggtgccatt ctatcaaaaa tttttacaga tgcttgtatt ccaccttcat
    13321 ctagaggatt tctcaattct cgttgtaatc gctctaaact attaatcgtt gtgttaatgg
    13381 atgtagtcat gtttctaaat gcaggtgtca ttccgtcgaa aatctttatt gaagtttgta
    13441 ttgtagccat ttcctcactc tcctttcttt tttgaaataa taaaagcact tacttatttt
    13501 taagtaagcg cttatgtatt ataaattcaa tagttctttt ttcttaatat taaattcttc
    13561 ttctgttatt gctcctaaat ctaataaatt tttatatttt aatatttcat ctgcattaga
    13621 attattgcta ttattttctg tatcttttaa aattatagat aaaacagata aaatttcttg
    13681 tgcatcttca tacgctcttt catatccaat agaatttgtc tttgtttttc cactaattaa
    13741 atttatatat gccacaggct ctgaaatagc ttttaatgta agctttattt gtaaattttc
    13801 aattatcttt tttgtttttt tcttgcctac aacgctacca acaatagctc ctgtaccacc
    13861 aaacaagact cctcctgcta aagcacttcc aataccacct tttgttacag tctctccatc
    13921 ttctagcaac tcaaaacctg ttatatcttc aaaactataa acattcttct taatttttcc
    13981 attttttctt ttagtttcta tatatatgag tttattagtt tcgtcaaact ttataaaact
    14041 gttaatttct ttagttacat taaatttttc aactaaaaaa tcattttgtt tcttagaatc
    14101 tagaacactt ttaatttcag attttgtagg taatccatct ttaaatgtaa attgtttgtc
    14161 tttgtattcc atataattca ttagacattc tatgcaaact gaaccatcct ttattttttg
    14221 gtctgtcttt tcttttccac atatacagca cggttccttt gattttctaa ataatcccat
    14281 aatattaccc ccatataata attttataag attattatac tatataagta aaatttttac
    14341 atcataatca cctcctttca ataaaaaaac acctactatt caagtaagtg tttttagcgt
    14401 ttttaatttt aagtccacat agttaatata aaacattttt aattgaagtc caaatttgag
    14461 aaaatgtgta atttacatac cacatagtta atataaaata attatgggta gattttaaag
    14521 ataaagtaaa aaatggattt acataccaca tagttaatat aaaataaggt aatactgtaa
    14581 tagaaccaga atttgattat ctatttacat accacatagt taatataaaa tcccaaaata
    14641 aacttagcat ttccaatacc tacacatacg catctctctc aaatttgcag tgaaccatga
    14701 gtagtgcaat tgataacatt tatcacacac cctcaacgcc ttatatttca atgcttaagc
    14761 cttaatttct tcaaaaaatc gaacactgca aaatttctat gcctttatta tatcataaaa
    14821 atattatttt tgaatatctg taccaatttg tggtataata aaagcaagaa gaactacaat
    14881 ctatttgaaa ctagagtgaa gttctaaaaa ttaatgttta tttcttttaa acttgattgt
    14941 aagtttaaaa tcaaagtcac tcttagtcgt gtttgagtgg ctttttactt ttttaaataa
    15001 cttactagtt aagtaaacta ttaaactggc aactaaactt gctagtacac cttgtaaaaa
    15061 attatccata catactcacc tcccttctat acgttgggag gataatcttt tgtatgaact
    15121 ccactctata aattgtagat tacatcttct tgctaaaaat attataacat ataattctta
    15181 catattttac ctatatttta tcttcttcta cttcttctag cttctttagc agtctttttt
    15241 tcttctttta tttcttcttc tactttaata tctatagaag cagcaacaaa tgccctctcg
    15301 tagtctggta aatctgtata ttcatgtggt ttccatttga atttatgaag gcaataatga
    15361 gctgcactag catcataatc gcctccttca attagttttt tgcttcttct actttatcct
    15421 caaaagtcct atcaaaacca tttacctctc ctacttcact tgaaaggtct gtgtattcgc
    15481 caggagttaa cattgttgtt aatagttcct ctgctcccat tacaccatag ctattttgaa
    15541 gttctgcatc atgtaaatct ggaaatacta tagtttccac acataatttc aaagtgtaag
    15601 tattaaaatc tgtttcacta gtgtattgcc ctgttgcttt tccttttttg cctataacag
    15661 gaactctaac agcagaattt tttctcaact gtctatctct gtctgaatct attgctttaa
    15721 gttcccattc tattggttta ccatcctcac ctataaacct ttcacttgcc acatactttc
    15781 tattctctac ttttattgca ttttgactta aaaaagcgtt taaatctccc atattattct
    15841 acctccatga ttaaatattt tgtttctttt tctattgttg caatgccatc ctcatctagt
    15901 ttgattttta ttgtaattgg ttttaatgat gaaccttcaa ttgtatcaag tgccagttta
    15961 tcagcaatat caactaaaaa tagacctgct tttctataca tttctcctac acattcttct
    16021 ataggtcttt tcttactgta atcaaaagaa attgaatctt ttatctcata tttatcctta
    16081 attttaacca tctcctattc ttataaataa aaaatacaca tttatgattt atagatgtgt
    16141 attttactcc ataccatttg ctatattgaa cttctcgact attctccaat tctcaaaagt
    16201 aaaatctaca tcctcatcca aatattctcc atcagcgtca aatttagcaa ttatacctga
    16261 atctaggtta caatcttcaa gtattatagt ttgacgacct actgaacttg ttgggtcttc
    16321 gttagtaatt tgcatatcaa agtaaatatc ctcgcctgtt tctttatatt catataacaa
    16381 ctctctaaaa atagaagtat tataataaaa ggtagcactc cctgaaaatt tacttcctgt
    16441 acttttattt ccttttgtaa tgctacctaa aattggcatc tcacttttat ttttttccat
    16501 cttagcttct aagttaatag cttgcataaa attatatctt ttgcctttta ttgttacaaa
    16561 acattcagct ttagaaccac ttattgcatc tctaacattc atagtaatat ttttagacat
    16621 ctatatcact ctcctttctt aactaactga aacagtcata taaagcttac ccatagcact
    16681 aataactttt acagcatcac ttactacaac agttttcttg tcgcttccag gttctacaga
    16741 aacatcatca gctttgaaat cttctattgc tctcatattt tgcagttgtt catgatgctt
    16801 aactacatca ttccaaaacg agatacgacc agatttatca tttggtactt cgcccaagta
    16861 ctttgtatta aataaagtcg ctatatcatt agcaatctgg tcaagtactc taatactttg
    16921 attagatgaa aaatcgtcat tcttatcgtc tgtaaaactt acaaaagtat ttatgtcctc
    16981 taatacatga acttcatctc ccaccttgtg gaatataaat ttaccagttt ttaaagcttc
    17041 ttcaagttgt atttgagtat aattaacatc aacatcaaac tcaccatcat acttcttatt
    17101 tgtattagat ttatttatgt cacatcctgc tatagctcca gtagtccaat aaactaaact
    17161 agattctaat aacccagtat ctttaatctt attttctaca gatactacac cttcataatc
    17221 tgcatcattt ttcttatata gtactgtttg aaacttagct cctaccttat ctctcattct
    17281 ctttgtaaat tctacaaaca aacttttaat ttctgctgtt gtagccaaac accctagtgc
    17341 attaaatgaa taactttcta ttttatccaa gaaagcttgg tactctgctc ctgtcacagc
    17401 ttcgccatta gttccaccag taaatacaag tcctgcgctt gcttctaatg ttgcatcctt
    17461 cttccaagtg atatagtcat tatcttgtaa gtctgtaatc actttagcta tttgagtatc
    17521 taccttctta ttatctaaaa gtgttacaac atcaaactta gcattatcat ctatatttgt
    17581 tgtaactgtt acttttaagt catttcctct gataccacta tattttgctg tggctatagt
    17641 acaactggct ttaacgcctt tatttaattt ataaaaatat cccaacctta tatttttgaa
    17701 taaatctctc aaacctttca gcttctcatg agtataatca tatccgaaat acttcactga
    17761 atacttctca aaatcatcac tggttacttg aaatacgtct tcatctatgc cccaatctaa
    17821 ctctaaaggt attgcaacaa tacctctatc cgataatgaa ctggttgccc tcttagctga
    17881 gataaaattt atatagctac ctggtaatat tttattctgt gttacaaatg ttcctccacc
    17941 taacgccatc tagctcactc ctttcataaa tttatttatt atttcctcta cctctgagaa
    18001 ggaatataac tcattttctt ttaaaattgc atttaataag tcttttctat ttacatactt
    18061 cttagaatta actatttgct ccttagtaaa cttgtagtca tcttctttgc ttaatgtttt
    18121 attcaaaatt atcacctctc ttcaaaccac cgaataactc tactgtatcc atcttattgg
    18181 tatcattatt ttttatagta aaatagttat aatcaacaaa gaagtgaaga acattgtcta
    18241 taatttcaaa attcatattt gtacctctga ctaaatctcc atcaatttct atatactcta
    18301 attcctccag tagcatctca gctatctcat ttatttcaaa attcttagct tctgaacgag
    18361 ggaaataatg tacatcaaaa gaatttttct ttaattctct cccgcttgga tatggtgtct
    18421 tgcttggatt taaaggaaca ataaaaaaac aaggttcatt aataccttgc tctacatcct
    18481 cactataaat tgtatatttt tctccaaatg atttatctaa ctttactgat attccatcta
    18541 taatattatt aagcatcaaa tactccttta agtaatatta ataacttttt ctctataatc
    18601 ttatcaactt ggctttgtag ttccatctct gaaattgtta agaaatgttg tcctttaacc
    18661 caaccttttc catctttagt tctatgcccg aaatttacat aacttgcata ttcagtcgga
    18721 ttaacaacct ctataatata attatttcct tgtttataca caggaagcga cctagcataa
    18781 gccactccat tccatccttg tcttaagaat cctgtatcaa ctggtgtcct tctaattact
    18841 tttcctaaga gtcttgctgc taattctctt gctgcatctt tgcaaaactt atctaaatca
    18901 atctttgtaa gcttctccat ctttttacaa actcttttaa actctctaaa atcaacactg
    18961 ccccatctag ccattatgct ttatccttaa ataactcaa
//
LOCUS       DAYATN010000067        18381 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.067, whole genome shotgun sequence.
ACCESSION   DAYATN010000067 DAYATN010000000
VERSION     DAYATN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.067"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            428..1735
                     /locus_tag="ACGI8A_002229"
     CDS             428..1735
                     /locus_tag="ACGI8A_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180225.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1821..2083
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2209..3012
                     /locus_tag="ACGI8A_002230"
     CDS             2209..3012
                     /locus_tag="ACGI8A_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0180226.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3050..3661
                     /locus_tag="ACGI8A_002231"
     CDS             3050..3661
                     /locus_tag="ACGI8A_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFH0180227.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3693..4364
                     /locus_tag="ACGI8A_002232"
     CDS             3693..4364
                     /locus_tag="ACGI8A_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFH0180228.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4663..5025
                     /locus_tag="ACGI8A_002233"
     CDS             4663..5025
                     /locus_tag="ACGI8A_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFH0180229.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            5461..6162
                     /locus_tag="ACGI8A_002234"
     CDS             5461..6162
                     /locus_tag="ACGI8A_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HFH0180230.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDELCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            6433..7251
                     /gene="larE"
                     /locus_tag="ACGI8A_002235"
     CDS             6433..7251
                     /gene="larE"
                     /locus_tag="ACGI8A_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HFH0180231.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            7306..8454
                     /locus_tag="ACGI8A_002236"
     CDS             7306..8454
                     /locus_tag="ACGI8A_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFH0180232.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            8590..9978
                     /locus_tag="ACGI8A_002237"
     CDS             8590..9978
                     /locus_tag="ACGI8A_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0180233.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            10007..10705
                     /gene="walR"
                     /locus_tag="ACGI8A_002238"
     CDS             10007..10705
                     /gene="walR"
                     /locus_tag="ACGI8A_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism DNA-binding response
                     regulator WalR"
                     /protein_id="HFH0180234.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            10708..12141
                     /gene="walK"
                     /locus_tag="ACGI8A_002239"
     CDS             10708..12141
                     /gene="walK"
                     /locus_tag="ACGI8A_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFH0180235.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            12163..12900
                     /gene="truA"
                     /locus_tag="ACGI8A_002240"
     CDS             12163..12900
                     /gene="truA"
                     /locus_tag="ACGI8A_002240"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFH0180236.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    13011..13218
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13471..14670
                     /locus_tag="ACGI8A_002241"
     CDS             13471..14670
                     /locus_tag="ACGI8A_002241"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFH0180237.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            14917..15963
                     /locus_tag="ACGI8A_002242"
     CDS             14917..15963
                     /locus_tag="ACGI8A_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HFH0180238.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKPHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTGQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            16071..17234
                     /locus_tag="ACGI8A_002243"
     CDS             16071..17234
                     /locus_tag="ACGI8A_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0180239.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(17323..17703)
                     /locus_tag="ACGI8A_002244"
     CDS             complement(17323..17703)
                     /locus_tag="ACGI8A_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HFH0180240.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(17706..18365)
                     /locus_tag="ACGI8A_002245"
     CDS             complement(17706..18365)
                     /locus_tag="ACGI8A_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFH0180241.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         7401 a         1770 c         3192 g         6018 t
ORIGIN      
        1 aaatttttag aaaaagtaca atagtgttaa tgtgtgaatt agataaggtg tatataatag
       61 tttagatgat atgatataaa atatgttgta aattaaatgt tttctttgac aatatataca
      121 caaggatgat aatatctata tgaataaaag atagtgtgta atttgataat atgtaattat
      181 aacattatga tataattaca ataaagtatg taataatctg tagaaatttc aagctagtta
      241 tctaagtttt cggataacta aatctaaacg gaatacataa tgtattaaat aaatgatgat
      301 actaaaatga tactattttt atagaaattt attgaggtat ataattaatg aaaataatat
      361 attaaattat ataaattaaa ttatataata tattgagttt tacataaaag caaaggtggg
      421 aataaaaatg aaagaaattg ctgatgctat aacatcagag tatttaagct atggtatcaa
      481 tgaagatatt agtatctatg aaggtagatt ttgcatatat ttagataaaa aatatagatg
      541 taatgggaaa atttattata aaatgacacc tccaatatct ataagtttta aagcagacat
      601 aggttgtgtt gaagagatag acaatgaaga tgacaattta gctttagatt atgataatgc
      661 tattcttgaa gttcatggat ataaaattat ttctataaca ataaatactt taagtgaatt
      721 tagtgtagaa ggttatatca atgatgactg tataaaatct aagaactcct atgttgaata
      781 tgtagatttt aatataataa acttagataa aatacctgga aaactcatta aatacaatga
      841 taaggtatac gcaggtagaa ttgaatttga tataaatgat tatgttgtaa ctatagacaa
      901 gcgatatgat tatagaaaag aattaaaatc agaattaaag tctaaatctg gagcaataat
      961 aactcatata ggcaggatta gaagaaaaga tggtagaatt tttagaacaa ataatacaat
     1021 aaatctttta gataggatat caacagcttt gagttttatg tgtggaagat atgtagggtt
     1081 ttgtttggcc aaagggtata gaagtggtaa tgaagtttat agaatatgga atgaaaatca
     1141 aatatctcct tttagatatg taccaacatg gtcagataca ctttcaaatt atcataatat
     1201 ggaaaaatat ataagcctta tgtgtaaaaa gctagaagat ttttattatg gttctgctat
     1261 aaagagtgtt gttgattggt acatagaatc tcttggaagt gctaccatgg aaaacaatat
     1321 aatatctgta caaattgcac ttgaaacttt gtcatatgta attctagtgg aacaaaataa
     1381 gatacttaca gatgaagcat ttgactgtaa tttggcatct aaaaatataa gactcttact
     1441 tgatacatgt aagataccat atggtaaaca tgaattaaat atatttgata atataataaa
     1501 aaataaattt gatgatggag tggatttagt tatttattta agaaatagca tagttcatcc
     1561 aagtagaaag actcatagag ctgtgttaga ggttgaagat atttggaata taatttcaat
     1621 aggaacgaga tatattgaac ttgttttact ctttatttta ggttatagag gagaatattc
     1681 aaacagatta gtagaaagat gttatggtga agtggaagtt gtaccttgga actagatttt
     1741 tatatttatt ttaaaatata tattgacata tattgcctta tgctataaaa tcaatatcat
     1801 aataataatt cagaataaca ttaaatgcta agaaaagaaa tagtaaaagt agttaatctt
     1861 aaacagagag tttccgattg gtgagaggag caatagattt tactttgaac acatctttga
     1921 gcaggactct gaaagagtag aaatacttta ttaggagatt tccggaatcc ttgcacccgt
     1981 tatagtgcta gagtataata attttattgt acttaaagag gttagtgccg tgaggcatta
     2041 atgaacagag gtggaaccac gagagttaac tcccgtcctc tagcagaagc tagaggcggg
     2101 agttttttaa tataattttt taagcgcgca gagaattata ttgcaataga tacacttttt
     2161 aaaaaataat caaaatattt taaaaattaa aagacgaggg agaatattat gaaaaatata
     2221 ttaaaaaaag taggaatatt tacaattatg ttaggtttat taggtggagt tgtaggttgt
     2281 tcaaaaccag ataatgaaaa agataaggat gcttcaaagg aatctaaaaa agaagtagtt
     2341 gtagggtttg ataatacgtt tgtaccaatg ggatttttag atgaaaaggg agatacagta
     2401 ggctttgacg tagacttagc taaagaaaca tttaaaagac ttggaatgga agttaagttt
     2461 cagcctatag attggtcaat gaaggaaact gaattaaatg attcaaaaac tgtagatgta
     2521 ttatggaatg gatattctat aacagacgaa agaaagaaaa tagtatcata tacagaacca
     2581 tatttacaaa ataaacagat aatagttact ctaagtgact caaaaataaa ttcaaaagct
     2641 gatttaaaag ataaagaagt tggaacacaa caaggttcaa cagcacttga tgcagtggaa
     2701 aaggataaag attttatgaa tagcttaaaa ggtggagcac cagttcttta tgatacttat
     2761 gataaagcac ttagagattt agagataggt agaactagtg cagttgttgg tgatgaagtt
     2821 ttaattagat actatatggg acaaaagggt gaagacaaat acaaggtatt aaaagacgat
     2881 tttggattag aagattatgt tgtagcaact tctaaagaaa atccagaact ttgtgagaaa
     2941 ataaatgaaa cattaaaaga aatgaaaaaa gatggaactt ttgacaagat ttatgataag
     3001 tggtttaaat agaagataag ttagctgtta agaagggggc actaaaggca tgttacaagg
     3061 tttagagata gttatagcaa tgttttgtat aacattgata gtatcaattc cattggggat
     3121 tggagttgca tttttaagat tatcaaagaa taaattagta agtggtatta cacaatgtta
     3181 tatattgata atgagaggta cacctttact tttgcaaatg atagtcatat tttacggatt
     3241 acctctttta ggaattgtat ttgacagatt tacagctgga gtagtagctt tcttcttaaa
     3301 ttatgcagca tattttgcag aaatatttag aggtggaata cagtcaatcg atagaggaca
     3361 atatgaagct tcaaaagtac taggatttga taagtttact atgtataaga gagtaatctt
     3421 tccacaagtt tttaaaagaa tactagcacc tatttcaaat gaagtaatca cattagtaaa
     3481 agatacatct ttagtatata tattaggatt aaatgatatt ttaagaatat ctcaaatagc
     3541 aatgaacagg gaagcaagtc tgttaccatt atttgaggca ggagcgatat atcttatatt
     3601 tgtagctata cttactaagg gatttgaatt acttgaaaaa aaatattctt attatagata
     3661 aagttacaat attataggag gaggtttttt atatgttgaa aatcaaaaac ttaaataaat
     3721 catttaaaaa aaatagagta cttaaagata tttcttttga gttagaagaa ggtcagattg
     3781 gagttttact tggtaagtct ggtgcaggaa agaccacaat attaaggtgt ataaatggtc
     3841 ttgaagaatt tgatagtgga gagattataa tagataatga ggtaataaaa aataagagag
     3901 atatggcaaa aatacgagga aaaataggta tggtttttca aaatttcaac ttatttcctc
     3961 atatgacagt ccttgaaaat ataattgaat ccccagttaa tgtttttaaa gttcctagaa
     4021 aagaggctga agagagggct agagaacttt taagattagt tgatttagaa gataaattaa
     4081 attcttatcc atttgaattg tcaggaggac agcaacagag agttgctata gctaggtcat
     4141 gtgcattgat gccaaaagtc ctttgttttg atgaacctac ttctgcactt gatattgata
     4201 ctattcaaag agtagtcaat ataatgaaca gacttaaaga caaaggtatg acaatattaa
     4261 taattacaca tgatgttgtc ttctcaaata atgttgcaga taaaattata agtataaaag
     4321 atggtatagt agaaaatgtt caaataaaag aaaaaattgt ttagagtttt ataaatataa
     4381 actagggtag aattttataa tataaataaa gggcaagtaa aatcaaatat actatgccct
     4441 tttgttatct caaaatttaa tcttatattt tatgtttaag ttacattata atttataata
     4501 aaaataaaag gactatttaa atatataatg tgaataaatg tttaataatt tgaaatatga
     4561 taatttttta tgttgaaagc ttataatgat tatgatataa attggttata gtatacaatt
     4621 tatgggtata acaataaaaa attagaaagg aatttttata aaatgattaa attatatgga
     4681 tatacaaaat gctctactgt taaaaaagct aaaaattggc ttaaggaaaa taatttagaa
     4741 tttgaagata tagatatggt tcaaaaccct cctagtaaag aggagttaaa atcaatttat
     4801 aagactagtg gatatgatat aaagaaattt tttaatacaa gtggtatgaa atatagagaa
     4861 ttgggtctta aagatattgt aaaaactgaa tctgatgata aacttttaga aatattggta
     4921 agtgatggta tgcttattaa aagaccatta cttttggatg gtaaaaatgt attattgggc
     4981 tttaaagaag atgtatggaa aagtactttg ctaaaagaag attaggaaga gataggtgtg
     5041 ttgattaaaa ttattaatta atacacctat aaatttatgt attaaatcta atttttataa
     5101 atattaatta gcaaattcaa atcaaaaaaa ttcatatcaa caaacatatt acttcttatt
     5161 acaaattata tcatatgttt tatattaaaa tattataata ttacaacgaa ttgttacaaa
     5221 attacttatt attaaccatg aacatttgtt ttgtgctatc atatttatat aagggaattg
     5281 tcataaaaaa tatcttgttc atacagtttt atatagaaaa aagaaatgaa tcttttcttt
     5341 atataagtat attttgtaaa ttgcctctat atttatagtt taaataatag tggtattaaa
     5401 aatctgacaa caaaaataaa actttcaaaa aattaaatct aaaaagaata ggagataaat
     5461 atgagtattg aagatttata tgattttgaa tgtgttcctt tgaacaatga gattgatatt
     5521 gatgttttat ttgaaaagat gatagaagat ggaaaagaaa aagaatatac accaattata
     5581 gtcattgatg aaaaagtagg gctattaaga aaaaatatag atatgattac taaagagtat
     5641 ggttctcttg aaaactatag agagtattgt ttaacaaaat ataatgaaat tgatgtaaat
     5701 caattttttg attttaggaa gagtgagatt tcagaattat taaaggagct aattgattta
     5761 gaagatgatt tttatgatta taaacatttt agaccaatta tgaatataga tatatttcat
     5821 gaatataaca aagtttacat agctaaaatt ccaacaatta aaccatatga agtatttgca
     5881 tacataccta ttggcggatt taatgattgt cctagagatg aagaacatat agctattgca
     5941 aaatattggt atgaaaagca tggaatcttt ccaatcgcaa ttggatgtga tacagttcaa
     6001 tattctgtaa aaaatttaac taaagatggt aaaaaatttg atgaattatg tattgaatta
     6061 gtattttatt gtgaggatat tattatacaa gggtatgaaa ccttaaaggc tcttaaggat
     6121 gtattaaaac gctctacaat agtgcttttt tggtgggatt gatattccat catttagata
     6181 gtattaaatt gttagtcacc aagaatccac ttgctttagt tatggggagt gtgtcaaact
     6241 aggtttttat acacagtgta gcatagtaat atgtttaaat tacaaaggtt aaaaaaatac
     6301 tttaatattt ttaatattat aattaataga tttaaaggta attataattt ataaaataaa
     6361 tactttatat gttataatca ttaagaatgt aaaaaaataa tatatagttt tatagttcat
     6421 atttttccag atatggaggt aaattttgtg aaggaaaaag aaaaattaga taaattaaaa
     6481 aaaatgttac ttgaattagg cagtgttgta gttgcttatt ctggtggagt agatagtaat
     6541 tttttgctta aggtcgcaaa agatacatta ggtgaaaatg ttatagctgt gactattcat
     6601 gcaatgatgc attcaagtag ggaaattgaa gaggctaaac aatatactca aaattttggt
     6661 gttaaacata tcatattaaa tatagaaaac tttgatttaa aagaatttaa agaaaatggt
     6721 gttgatagat gttatcattg taaaaaatat atattctcta aaataaaaga ggtagcaaaa
     6781 gaacataata taaagtacat agtagatgga acaaatatag atgatttagg agactataga
     6841 cctgggctta aggcattgag tgaattagga gtaattagtc cattaaaaga tagtagttta
     6901 aaaaaagaag aaattaggtc gctttcaaaa acattaggat taaatacttt taataaacca
     6961 tcatttgcat gtcttgcgag taggatacct tatggagttg aaataacaga tgaaaattta
     7021 agaatagtag aaaagagtga agaatatcta tccaatttag gtttttctca atttagagtg
     7081 agaatgcatg gagatatagc tagaatagaa gttggacagg aagaactagg taaatttttt
     7141 gaaaataaca attttaataa agttgatact aaattaaaaa tatttggctt taaatatgtt
     7201 acactagata tgtcaggata taaaatggga agtatgaatt taaatgtgta gattatttta
     7261 aaaataaata agttttatag ttaatataag gaaacaggag gttttatgaa aaattatacg
     7321 ttaatatcgc catgtttttt tggaatggaa aaaatgctag ctagagagat tactaattta
     7381 ggatatgaaa taataaaaac agaagatgga agaataacat ataaaactga tgagtttgga
     7441 atagcaaagt caaacatgtg gttgagatgt gctgaaagag tacatcttaa aattgctgaa
     7501 tttgaggcaa aaagctttga tgaattattt gaaaatacaa aaagaataaa ctggtctaga
     7561 tatatacctt atggagctca atttcctata tcaaaagctt cctctataaa atcaaaatta
     7621 tacagtacac cagatgtaca agccatagta aaaaaggcta tagtagaaag cttaaagaaa
     7681 agttatttgg aggatggttt gcttaaagaa gataaggaga aataccctat ttttgtattt
     7741 atacacaaag ataaagtaac catttctata gatacaacag gagatgcttt gcataagaga
     7801 ggatatagag aaaaggcaaa taaagctcca ataagagaga ctttagctgc tgggcttatt
     7861 tatttaactc cctggaaagc aggtagagtt ttagtagacc ctatgtgtgg ttctggtact
     7921 atattgattg aagctgctat gattggtata aatatggcac ctggcttaaa tagagagttt
     7981 atatcagaaa agtggagaac tcttgataag aaaatatggt gggatgtaag aaaagatgct
     8041 tttaataaga tagataatga atctaaattt aagatttatg gatatgatat agatgaagaa
     8101 tctatagata tagctagaga aaatgctgaa atagcaggtg tagatgaata tatagagttt
     8161 aatgtaggag atgcaactca atttaaaagt gaagatgagt ttggatttat aatcactaat
     8221 cctccttatg gagaaagatt agaagataaa gacagtgtta aacagttgta taaggagctt
     8281 ggttatgcat ttagaaaatt aaaaaattgg tcgtattatt tgataacttc ttatgaagac
     8341 tttgaatatg agtttgggca aaaagcagat aaaaaaagaa aattgtataa tggtatgtta
     8401 aaaactaact ttttccaata tccgggtcct aagcctccta gaaataataa ataagtttta
     8461 catgatttat ttgtaaaata taaaatttta ttaaaataag taaaacatta tgatgccttg
     8521 aaacgtctaa ttattgtgag gagatttttg tgaacaaatt taaagattta taggattagg
     8581 aggagttaaa tgaaagtgtt aaagcttatt actgtagtag ccctgatgat gttatttgtt
     8641 actggatgta atataaaaac tgaatctcca gaacaattag cgaagatacc tgactatgat
     8701 gatacaaaaa aagtattgta tgatggtatt gaccagttac taaaaccaga ttcatctatg
     8761 atattgcctt ctaatacaaa agaagtagga aaaataaata aggtagattt aaacagcgat
     8821 ggaattgatg aattagtagt atttgaacaa aaagaagatt taagtaataa tgtgagtcag
     8881 gtagggtttg taaccttgag ttacaatgga gaaaaatacg tacttggaga ccatttttta
     8941 gaaaatggcg aatcaataga atatgctaat ttttatgatt tggactcaga tggatataag
     9001 gaagttatct tattggttaa atccaaagac aaaacgaata tgcatatata taaagtagta
     9061 gataatgaaa taacaaaaat atatgacttg gatgcatcat ggcttcaaaa taaagaagat
     9121 tttaatgata tgaaagtaaa aattggatat atagatggag atgacaagtt agacatattg
     9181 atgcttcatt tgaataataa gactaatgaa atgtttgcaa gtgttgcaaa ttttgatggg
     9241 aaaatgaaaa tcaaagattc tgttaagttt gaaaatgtaa aaaacttaag tgaattatat
     9301 ataacattag gtaatgttgc ttcatctgtt ggaaattctt ctgcagctat aaaaggtatt
     9361 gtacttgata ttcctatatt gaaagataat aactatatta cgcagatact ttatatgaaa
     9421 gatgggaaaa ttaacaaagc ttttagtgat tatgataaaa ctattactaa atcttattat
     9481 atacctgtag atgatattga caaagataag attatagaaa tacctatagt tgcaggcagt
     9541 acaggtaata ataaaaatac ttatagttct aaatcatctg caactataag ttggtataga
     9601 tggaatggaa aagaaggtgc tgactcaagt ttaatattta caagtcagat atattataat
     9661 tataaatata attttaaatt atttattcca aataatttat ttgataaaat acttgttgaa
     9721 caggaatttg ttggagaaaa agcagtattt aaattttact attttgacta ttcagaccgt
     9781 aagaatttat ttaatatagt agtagagagt aaaaataaac ttgaagatag aaaaaataca
     9841 ggtactcaaa attctattgt acttcaagag agcgatgaat atactttctt actagttgta
     9901 aacaatgcaa atgaaatgga taagctagat atgactatag acgcattaaa agaatatttc
     9961 tcattaatat atggttaata ttatactcaa ttggaggttt tattttatga aggaaaagat
    10021 acttatacta gaagatgaaa ttggtattag aagttttgtt agtataaact taaaaaggga
    10081 aggctatgaa atagttgagg caggtactgg aagagaagct atagaaaaaa tgacaacaga
    10141 aaaggatatt acaatagctc ttttagatgt aatgcttcca gatataagtg gtatagaagt
    10201 atgtaaattt ataagggaga attttgacca agtaggtata ataatgctta ctgctaaagc
    10261 tcaagaagat gataaaatag aaggatttat ctcaggagca gatgattaca tcataaaacc
    10321 atttagtata aaagagcttt tagtcagagt ttctgctctt cttagaagag tagcaaagga
    10381 tgattcaagt gttaagtcga gtgagatagt ttctccacca tttatacttg atatagacaa
    10441 aagaaaatta tttaaaaatg gaaaggaaat tgagcttaca ccaactgaat tttcaatagt
    10501 gaaatatcta atatcaaatg ctaagcagtc gttaagtaga gaccagatat tagacgaagt
    10561 atggggaact aactatttat atgattttaa aatagtagat gtaaatataa gaaggataag
    10621 aaataaaata gaagatgacc catcaaaacc aaaatatata caaactattt ggggttatgg
    10681 atactgtttt agaaaggaag aataatagtg ttacattttg aaggtctgag aaaaaaagta
    10741 ataaaaaact attttataat aattattata atggttactc tgtttgaagg attgttcatg
    10801 ttttacatac aaaactacta ttatgattct gtcaaacagc tacttgaatc tgaaataaaa
    10861 tatgctgatg aatataatgc tattacaatg gaaactacaa gttttgagaa aaaagtaaaa
    10921 aatatatttg ataaacaacc cttaactaaa aattcagaat ttggaatttc aataatagat
    10981 aaagataaaa atattatatt agaccaatat ggttttaaaa gtaaagaaaa agcaaactat
    11041 gaagatgtta acaatgcatt aaaagatata aaaactaaaa atttaacacc ttatacttat
    11101 agaataccag atacagggga gcatgttatg agtatctcct taccacttaa ggtaaataat
    11161 ataatagaag gtgttgttag atatacagta tcattagatg ctattgataa tgcaatatta
    11221 aagcaagcta catggcttat attagcagga atatttatac ttattatagc tatactaata
    11281 agtcttaaat ttgctgagac tcttataaaa ccattacgag agctaaagaa atttgcaaat
    11341 gaattggctg taggaaatta taatataaaa ttagaaaaga tgaagattgt agatgatgaa
    11401 attggtgatt tagcacagac atttgaacac atggcacatg agattgacaa aagtgaaaaa
    11461 ttaaaagagg aatttatatc atctgtatct catgaactaa ggactcctct tacatctata
    11521 aaggggtgga gtgaaacctt aggttatgaa agtataacaa gagaagaact agacttaggt
    11581 ttaggcatta tacaagatga aacagaaaga cttataaaac tagttgaaga attattagac
    11641 ttttcaaggc tttcttcaga caggataaaa cttcatgttg atatagttga tgttgaaggt
    11701 ttaattgtag gtgttgtaaa tcaacttaaa gttaaagcag ctgaaaaaga tatatcactt
    11761 ttatttgaat ttgaaaatga atttatagag aacattcaag gagataaaaa tagattaaga
    11821 caggttctca taaatttaat acaaaattca tttaaattta cgagtcaagg tggttacata
    11881 aaagttgtag cttctcaaga tgaagagata acaaccatat cagtagagga taatggttca
    11941 ggtatagaaa agcaaaattt aaataaagtg ttagataaat ttttccaaga agattataat
    12001 aaggctggta gtggattagg actagcaatt agtaatgaaa ttgtaaaact tcatggtgga
    12061 agaatgaaaa tagaaagtga aaagaatgtt ggaacaaaaa taacttttaa tataaaaaat
    12121 aaatttgcta aacaagcata aattcttaga aagaggatta agatgagaaa cataaaaatg
    12181 atagtagcat acgatggttc aagatataaa ggatatcaaa aattaggtga caataatatg
    12241 actattcaag aaaaacttga aaatgtttta agtaaaatga ctaatgaaac agtggagata
    12301 ataggttctg gaagaactga tatgggagct catgcaagag gacaagtggt aaattttaga
    12361 actaattgta tggattcttt ggataaaatt caaaaatatt tatatgaata tttaccagaa
    12421 gatatagttg taaaaactgt tgaagaagtt gatgaaagat ttcattcaag atacaatgta
    12481 aaatcaaaaa cttatatgta taaaattgac aataataagt atcatagtcc ttttataaga
    12541 aaatatgcta ctcatgtaag taaaaaatta gatttagata gaatgagaaa agcaagtgag
    12601 tatttagtag gagaacatga ttttaccagt tttgcttctt ctaaatctaa gaaaaaatca
    12661 aatgtaagag aaatttattc tataaatata aaagaagatg ataatgttat agaaatatat
    12721 gtagaaggaa atggattttt gtataacatg gtaaggataa tagtaggtgc tttaatagat
    12781 gttggtctaa aaagaagagc tccacaagat attaagtata tgcttgaatc aaaagataga
    12841 tgtcaatcat cagatactgc accagctaaa ggactatgtc tttggaaagt aacatattaa
    12901 tcaattatat aaaactctct ttaaaataaa aataaaataa attatatagt tgacataaat
    12961 ggaaaaataa actattatat attttataag agagatataa aactttatat ttaaatcctg
    13021 tgagaaagag agtaacttct atgtgtttta tagagagctg aggatggtgg aagcttggca
    13081 aataagtatt agtgaaaaga actttggagg atactaattg aatttagtat gttagtaggt
    13141 aatctcgcct taagagaaag agtggataag tttatcttat caattagagt ggtaacgcgg
    13201 atatatttcg tctcttggtt tttccaagag gcgtttttta atatatttaa ttattagtgt
    13261 ttatctggta tgttgaacat ttactaattg attagctaag tttatcaata agagtggtac
    13321 cgcggatatt tcgcctctta gtattttaaa atactaaggg gtttttttag tattaaaatg
    13381 attttatgtg agtctaaatt ctcagagtca ttcaatatta agaatttcta taaaataaaa
    13441 attataattt aagattggga ggaattaaaa atgaaggaaa aagtagtatt agcatattca
    13501 ggaggacttg acacatcaat cataatacct tggttaaagg aaaattatga agatatagat
    13561 gttatagctg tttgtggaaa tgtaggtcag gaagataaaa tggaagatgt atatgaaaaa
    13621 gctttacaaa gtggagcatc aaaggcatat gtggatgata taagtgaaga atttgtaact
    13681 gaaactatat ttaaagccgt aaaagcagag gctaaatatg aaggaaaata cttattagga
    13741 acttctttag caagacctat aatagctaaa aaattagttg aagttgctca taaagaaggt
    13801 gcaaaatata tatgccatgg atgtacagga aaaggtaatg accaagttag atttgaagct
    13861 acaattgcag ctcttgaccc gactataaaa gttattgcac cttggagaat ttgggatata
    13921 aaatcaagag aagatgctat agactatgct gaaaaacata atataaaggt aacagcaact
    13981 aaagctaaaa tatattcagt agatgctaac ctttggcatg tttcaacaga aggtggagat
    14041 atagagcacc tagaaaatga acataaaaaa gatgtttata aacaatgtgt agacccagaa
    14101 gatgcatgtg atgtagctga atatgtagaa gtttactttg aaaaaggtgt acctaaaaag
    14161 gtaaatggtg aagaattatc tccagttgca ctgattcata agttaaatga gttgggttgt
    14221 aaacatggaa ttggtgttat agatatagtt gagaatagac ttgttggtat gaaatcaaga
    14281 ggtatatatg agactccagg aggaacaatc ctttatgaag cacataatat tttagaaagt
    14341 gctactttag acaaagatac attacatttc aaacaaatgg tatcttataa atacggagaa
    14401 cttatttaca atggactatg gtattgtaaa ttaagagaat ctatagatgc atttatggaa
    14461 caaactcaag ataatgttac aggaacagta aaagttaaac tttacaaagg aaatataaaa
    14521 cctgctggaa tatttactga aaatgctctt tatgatgaag gaatatcttc ttttggaaat
    14581 agtgaacttt atgaccacaa agatgcagaa ggatttatta atttattcac tttaccatta
    14641 aaaatcagag ctatgaaagc tggaaaataa taaaataaat tatttaagag tgtataaaaa
    14701 ctatttttag gtttttatat gctctttttt tatttgaatt tattaactta tacagtaaat
    14761 agtataaaaa aatatattat agttccaaat ggcttaataa tgtgaaaata taagacctaa
    14821 aatataaatt ttataaatac aatagttatt gactctctca ttgtaagagg gtttatactg
    14881 aatctaatga aaatttatag tgaggagata gattatatga gaaaaataat tgaaattaaa
    14941 catcattcac atgattatga gtgtatgtgg aatggtatag aagatttata tataaataaa
    15001 accggtgaat gccttccaaa cgattttttc tttttactgt caggttttgg ttcattttgt
    15061 tatatgaaaa caaataaagc tgatttgaaa agaatggtag ctttgggaga tggaaggaca
    15121 aaacagatgt ataaattttt agcaccaatt gttggatttg aatacaaacc tcatgagttt
    15181 aaaaagtatg agcaagcatt aaagaaagca aaatctgaaa ttgatgatgg atatccagtt
    15241 attttaggag ctttagatat gtattatcta ccatattata agaagctata tcataaagaa
    15301 catattcctt ttcattatat attaatggta ggatatgatg atgaaggaat atatctgtat
    15361 gattgtggta gaacagaatt attacatctt tcttatgata aattacgtga aagcatgaat
    15421 tgcaattccc ctggattaag caaagaaaat actatttgca caataagaat gaatacttca
    15481 aagaataaat ataacattgc tagagaagct attgctataa agcgtgatat gtttttaaat
    15541 ccacctaaag gatttttggg atataaaggt tttgaaaagt ttattaaaga gttgccacag
    15601 tggaacactg aattggataa agatgattat gataagattt tgaccaatat ggttatgttt
    15661 tttggaacag taccaacaat tccaaatgca atcaaaggaa ttaaagaacc agatactgtt
    15721 caatttaaag gtggatttga taaaatgagt agaatgctca acagtattgg ggaggaatac
    15781 aaaaacgact cttttataca agtagctgat atttttgata aaactgctat tataattgag
    15841 aaaatatcca atattataat agattactta actcaaacat gtgatgatac aggacaactt
    15901 ccgttattgt tctctgaggt cttagagcat atgaaaacag gatatttaat cttggatgta
    15961 taaaaaatat attgttgacc ctaacattac gtaatagtgt aatatgaaaa tatagcttga
    16021 atatattagt aggtattgat ggctgtaaaa ttaaaatttg aggtgtggat atgagaacag
    16081 tgaaacaagt ttctgattta actggaataa gtgtgagggc attacattat tatgatgaaa
    16141 taggtctttt aaaaccaaat aaaattacag atgcaggata tagactttat gatgatgaat
    16201 ctattaaaac tcttcaacaa attttatttt ttaaggagat tgatattcct ttaagagagg
    16261 ttaaggaaat aatgtcaagc caatattttg ataaggttga agctctaaaa aatcaaaaaa
    16321 aattacttat tttaaaacga aagagactgg atgaattgat agagcttata aatcaaactc
    16381 taagaggaga aggaaatatt gattttaaag agtttgatat gagcgagtat tttgatgttt
    16441 tagaagaatt taaaagagag cataggaata aggtaattaa aatttatggt agtgtagaaa
    16501 aatacaatga atatatagaa agagttaagt ctaatgaaga gaaaattgct aaaatggcta
    16561 taaaacaata tggaactatt gagaagtttg ctaaagctat aaaaacaaat tttagcagtg
    16621 atatattgaa tttaggagaa aaatttgata gatataaaaa tgattgttta aaagaaaaac
    16681 atccaaaatt aaaagaatta tatagaaagc ttgtagaaga tttaaatagg aatccttctt
    16741 caacagacct tcaagaaata gcaaaagaga taacagatat atctaaaaaa gattatgaaa
    16801 ttttctctat ggatactggt gatgacaact ggtattatat ggttcaaaat tatctagtaa
    16861 atcctatgtg gatagaagaa gtagataaaa agtatggaag tggagcaggt aagtttattg
    16921 gacaagtact aaagacttat ctaagagata gaaaaccaaa gataaataca ttgtatgaaa
    16981 aacttgtaga agatttgagt agagattgtt cttcaaggga agttcaaagc attgttgaag
    17041 aaatagacaa tgaaatgaaa agaagtaatg aattttataa aatagacaat ggtgaaagat
    17101 actttgatta tatgtcagag ctttatttac aagattctaa ctatataaaa gtaactgata
    17161 agaattatgg tgatggtgca tctaagttta ttggagaagc ttttaagatt tattttgata
    17221 ataataattg ttgaaaattt ggaagtaaaa aatatgcaag ttttataaaa gcacaaaaat
    17281 aaggatgtag attagctcca tctatatcct tatttaaagt tattaaaata tatagtttac
    17341 atcaaactta acactttcaa taatatttcc caaatatgtt tttatacttt caacttcttc
    17401 taaagatttt aatttatttt cagtataaat tgctttgtcc agaatattaa accttaatcc
    17461 tatttcatca aagtactcat gatttaaaac tttgtaccaa tggttttttt ctttattcca
    17521 actcgtagat attgaatcag cttctttttg agctttttcc caatcttcat tttctataga
    17581 tttttctaaa acagatatat tgtctttata tccttctgta aaatcatata gtttattgtt
    17641 aacataaaat ccaaataata caaataagat agtccatagt ataacaaatg ttgtagattt
    17701 caatattaat cacccttttt attttgaata aatattttgt cattttcatc taaaacacaa
    17761 ataagaacat ctttaaaagt ttctatgtga ttagatttta aaatacccat taaccagtta
    17821 gtatcttttt gagctatgtc tagattttta tttattattc taccatccag tataagtggg
    17881 ataggtaaat gattaaaagc tcttggagga gtactattgt agcttgatgc tggtacaaca
    17941 cttagattac catcagtctc caaaatagca tattgtacat cttttaggtt aaagtatcct
    18001 tgaatcctaa gttgttctaa taattcatct atactaattc tttctttttt aagctcttta
    18061 tatacaattt ttcctttatc tattaataca gaaggtttac ctgataaaaa tgaacttaat
    18121 tttctacttt ttaatgataa gaatgaaatt agagtctgca tgattacaag tccagatatt
    18181 gctgcaacac catttatgat tggtatatta ttattttcca ttgggacaga tgcaacttct
    18241 gcaattaata gtgttataac taaatcaaaa cagttcattt cagctatttc accttttccc
    18301 atgactctaa gtgcaattag cactgttatg tataatataa tacttcttat taatactact
    18361 gtcatatcta tcaattactc c
//
LOCUS       DAYATN010000068        17815 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.068, whole genome shotgun sequence.
ACCESSION   DAYATN010000068 DAYATN010000000
VERSION     DAYATN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.068"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            142..477
                     /locus_tag="ACGI8A_002246"
     CDS             142..477
                     /locus_tag="ACGI8A_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0180242.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            614..1891
                     /locus_tag="ACGI8A_002247"
     CDS             614..1891
                     /locus_tag="ACGI8A_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein/redoxin"
                     /protein_id="HFH0180243.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            2012..3400
                     /locus_tag="ACGI8A_002248"
     CDS             2012..3400
                     /locus_tag="ACGI8A_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0180244.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEEL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3846..4484
                     /locus_tag="ACGI8A_002249"
     CDS             3846..4484
                     /locus_tag="ACGI8A_002249"
                     /inference="COORDINATES: protein motif:HMM:NF037435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HFH0180245.1"
                     /translation="MKSKEEIEWLLNRDYKIDPVVTFVGDRYQFSIRQRDALKRAVCT
                     DEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISIEVALSGGTLVIGSDGNIRDLAGLRG
                     TYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDAPVSNSGNLKYRILEHAKTWGIETEV
                     ELVKNADVVLEKLDRVVSSDAVIVDKCISYFNVARGIIEEYIKECNIINLNK"
     gene            4602..5471
                     /locus_tag="ACGI8A_002250"
     CDS             4602..5471
                     /locus_tag="ACGI8A_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFH0180246.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5626..6771)
                     /locus_tag="ACGI8A_002251"
     CDS             complement(5626..6771)
                     /locus_tag="ACGI8A_002251"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFH0180247.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(7104..7880)
                     /locus_tag="ACGI8A_002252"
     CDS             complement(7104..7880)
                     /locus_tag="ACGI8A_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFH0180248.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8462..9436
                     /locus_tag="ACGI8A_002253"
     CDS             8462..9436
                     /locus_tag="ACGI8A_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HFH0180249.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9534..9839
                     /locus_tag="ACGI8A_002254"
     CDS             9534..9839
                     /locus_tag="ACGI8A_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFH0180250.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9995..11155
                     /locus_tag="ACGI8A_002255"
     CDS             9995..11155
                     /locus_tag="ACGI8A_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFH0180251.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNLD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11205..12107
                     /locus_tag="ACGI8A_002256"
     CDS             11205..12107
                     /locus_tag="ACGI8A_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFH0180252.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12187..13080
                     /locus_tag="ACGI8A_002257"
     CDS             12187..13080
                     /locus_tag="ACGI8A_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFH0180253.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      13133..13314
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13542..14672
                     /locus_tag="ACGI8A_002258"
     CDS             13542..14672
                     /locus_tag="ACGI8A_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0180254.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTQEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14744..15769
                     /locus_tag="ACGI8A_002259"
     CDS             14744..15769
                     /locus_tag="ACGI8A_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFH0180255.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            15772..16968
                     /locus_tag="ACGI8A_002260"
     CDS             15772..16968
                     /locus_tag="ACGI8A_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180256.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(17096..>17815)
                     /locus_tag="ACGI8A_002261"
     CDS             complement(17096..>17815)
                     /locus_tag="ACGI8A_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFH0180257.1"
                     /translation="TISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGYCL
                     SNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLSNS
                     DIRGANLENSNLEYATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIPSD
                     AKRVSATSNACRCDKAKVLSITSVDGKESFKSARAYADENFIYRVGETIVAEDFNENR
                     WKESTTGIHFFLTREEAIGYL"
BASE COUNT         6850 a         1979 c         3095 g         5891 t
ORIGIN      
        1 ataaaatcta aaatagtttg taataggagt atatataata aaaattttaa ttaaataaag
       61 ctaggtatat ttataatatt aataaatata ttgctataaa agtttgttta ataaattaaa
      121 taaagaaaag aggagaataa aatgtacaac atagatgata tgatatataa ttgtccagta
      181 gaagctttat caaatatttt aggtaaaaaa tgggtggcta taattatatg gaaaatacaa
      241 gaagataaaa aacgatttgg agagctacaa agagctatac cagactgtag taagaaaatg
      301 ttagttcaac aactagattt tttaattgaa caggggatta tagtcaacca aaaaaagttg
      361 gttaataatg ttgtagagtc aacatatttt ttaagtgaat caggattacg attgcttcct
      421 gtcatggaaa aaatgattat gtggagcaat aaaaatttaa attgtgataa atactaaaat
      481 ttactctgta agtaatgaaa atttaattaa gctatgagta gaaaaatgta ttaaaattac
      541 ataatttttt agttagattt taatttagaa atgggattgt aatattggaa ataaaataag
      601 gaggaatttt aatttgcaaa atgtaaattt atttttagta tttatagaag gtatagtatc
      661 attcttttca ccatgtatac tgccaatttt gcctatttat ttgagtatat tgtcaaatag
      721 tagtgtagaa aatttaaaag aaggaaagac gagttttata ggaagttctt tatttaaaaa
      781 tactatattt tttgctcttg gaatctcaac aacattcttt atattgggtt catcagtaaa
      841 agtattgagt atgtttttta atgaaaataa agatttaata atgtttattg gtgggattat
      901 aattattatc atgggattat tttacatggg aataattaaa tcttcaatat tgaatagaga
      961 aaaaagattt aatgttaagt ttaaagaaat gaaagcaata acagctttta tattaggttt
     1021 tacatttagt tttggatgga caccttgtat tggtccaata ttggcatctg tattagtcat
     1081 ggtttcaagt tcaagtaatc atcttagtgc gaatctttta atagcagtat atactattgg
     1141 ttttattttg ccattcataa ttacagctat gttttacagt aaactattta aaactataga
     1201 taagataaaa tctaacatgg aaattattaa aaaaataggt gggattatac ttatagtatc
     1261 aggaatatta atgatggtaa atggatttgg aagtataagc aaacacttta atacgtcaca
     1321 aaatagtaaa attgaaagca agcaagaaga aaataagcga gaaaattcta ctgataaaga
     1381 agaaaattct gatggtaatg atagtcaaaa agactctaat aatgacaata atgataaagg
     1441 gtctaatgat gaagatagaa taaagtctat tgattttact ttaacagacc agtatggtaa
     1501 aacacataaa ttaagtgatt atgaaggcaa agttgtattc ttaaattttt gggctacatg
     1561 gtgtcctcca tgtaaagaag aaatgcctta tatagaacaa ctgtataaag actataataa
     1621 aaataatgat gatgtagtaa ttttaggagt agcttcacct aatttaggta gagaaggttc
     1681 aagggaacat gtagttaact tcttaaaaga ccaaggttat acattccctg ttgtacttga
     1741 tgaagatgga gcattagcat atcaatatgg aattaatgct tttccaacta catttataat
     1801 tgacaaggaa ggatatgtaa ctcagtatat tccaggagct atggataagg caactatggc
     1861 atcatttatt gaaaatcaga gaaataaata aaagtattta agtataagtt tataaatcaa
     1921 tttaaggtta caaaaaagtg cctaattgta ccacctaaat aatcttgtta tagtaaaagt
     1981 ataaacaata caagaaaggg ttgaatttta tatgaaaaaa acatttgatg ctataataat
     2041 aggatttgga aaaggtggaa aaacattagc aggggatttg gcaaacaggg gattaaaagt
     2101 agctttaata gaaaagtcaa acaagatgta tggtggaaca tgtgtaaatg tggcatgtat
     2161 accaacaaaa agcttagaaa attcagcaaa cagcgtaaaa acaaagaata taaattcttg
     2221 ggatgaagta caagctgaat atgagaaagc tatagataaa aaagaaactt taattacaaa
     2281 actcagagaa gctaactata ataaattaaa ttcaaatgag aatgtaacta tattcacagg
     2341 aatgggaact ttcattgatg aaaaaacagt tcaagtcaaa actgaaaatg aaatttatga
     2401 gttagtagca gataatatat ttattaatac tggttcaaga ccatttatac ctaatattaa
     2461 gggaattgaa aataagaata tagtatatga tagtgagtct cttatgaatc taagaacttt
     2521 acctaaaaaa atgacaatta taggagctgg atttataggt ttagaatttg ctggtattta
     2581 tagttcattt ggagcagaag ttacaatatt aaactctaat aatggcattt taccaaatga
     2641 agatgttgag gattcagaag aaataataaa attacttgca aagagaaatg taaaaattgt
     2701 taataatgca aatataaaag agattaaaga agtttctgaa ttagcaatag tagaatatga
     2761 agtagatggt aaatctaaag aattaacaag taatatgata cttgtagcta caggaagaaa
     2821 agcaaatact gaggagctag ggctagaaaa tgcaggtata gaattaaatg aaagaggatt
     2881 tattaaagtc tcagaaactc ttaagactaa taaagagcat atatgggcta taggagatat
     2941 aaatggaggt ccacaattta cttatatctc attggatgat tatcgcatag ttattaatca
     3001 gttgtttgga gataagacta gaacaactaa tgatagaaag aatatcccaa attctatatt
     3061 tataagtcca gcattttcaa gagttggtct taatgttaaa caagctaaag aaaagggata
     3121 tgaagtatta gtggcaaaga tgccagtaga agcaattcca agagctaaac agattggcaa
     3181 ggcagatggg tttataaaaa tagtgataga taaaaaatcc aataagatac ttggagctag
     3241 tatgatatgt gaaaattcaa gtgagattat ccatcttatt caattagctg tagatttaga
     3301 agtagagtat acatatttaa gagaccgtgt ttatgcacat ccaactatga cagaagctct
     3361 taatgatatt ctatctccta atatgataaa agaagtataa cataagttta atatataaac
     3421 ctaaaattat cattataagt agattttaga aagaggctat ctcaaaaaat agagataagt
     3481 ctctttttta ctgtttaaat gataaaaata tatttgattt tataaaagaa gattatctta
     3541 tgaactaaaa agtttatttt aaaacactac ctatttatgt tataaactta taaatataat
     3601 atagaatcta ttattctgta ttattaagtg gggttaagcg ttataattaa ttataaatat
     3661 tgaataatct aaatggataa gataatggta ttaaaaatta tcttaaaagg tgaaataatc
     3721 tgtatttaat gtagtacagt tatatcttta aagcaaggtg gtgaaatgat ataaataaga
     3781 tatctaaaag agggtttgat gatagtgata aaagatggtt ttcaagtaaa gaattaacaa
     3841 gacttataaa gtctaaagaa gaaatagaat ggttattaaa tagggattac aaaatagacc
     3901 cagtcgttac ttttgttggt gatagatatc agttttctat aagacaaaga gatgcattaa
     3961 aaagggcagt ctgtactgat gaaaaaaata ttataagaca atcaaaaagg ctttctctag
     4021 ataaaatcaa agaagatacc gtaaatatag atggctttaa tttgataata tccatagagg
     4081 tagcattatc tggtggtact ctagtaatag gaagtgatgg caatataaga gatttagcag
     4141 gtcttagggg aacctataag attatagata aaacagaaga agctttgaat ctgataggta
     4201 aattttttaa taaatacaat gcacagaatc ttaaatttta tttagatgct ccagtttcta
     4261 attcaggaaa tttaaagtat aggatattag agcatgccaa aacttgggga attgaaactg
     4321 aagtagaatt agtaaaaaat gcagatgttg tattggaaaa attagataga gtagttagta
     4381 gtgatgcagt catagttgac aagtgtataa gttattttaa tgtggctaga ggtataattg
     4441 aagagtatat taaagaatgt aatatcataa atttaaataa atagataaat ataggtgaga
     4501 atatataagt atatgtagca atattcttag taatacaagt aaagtttgaa aataggagaa
     4561 cgaatacata tcgaatttca taaaatgttg taaaaagaca tatgagagga tggatatgtg
     4621 tgaaattaac taaactaaat attaaaaaat atagagcacc tatatataaa tatgctttag
     4681 caaatgtaaa tttgaggtct gctaaatcta ctaattcaag tatcataaca gtaatacctc
     4741 agggagccaa aatggaagta ttggatgaag aagatgactg gattaaggtg atgtacaatt
     4801 ctcaagaagg atatgtgtat aaggatttgg tatcagtaag cgaatatgct tggagtaact
     4861 taaacttaag agaagataaa tctacaactt ccaatataat tacagtaatt cctgaaaaat
     4921 ctagggttga agtccttcaa gtagatggag attggagtaa ggttgtatat gatgataaaa
     4981 taggatatgt ttttaattat tttttatcaa ttgatgggaa taaaccaaat gaattagatt
     5041 ataagtattt ttatactgac atgattaaat ttgttaacga aaataacatt aaaagtacta
     5101 gcgattatct aattgtaaca gaccttagaa ataaatatac ttatattttt aagaaagata
     5161 atggaggatg gggacaactt tacaagtggc aatgtactat aggcaagcct gagactccaa
     5221 ctataacagg tatattttat ataagtggaa gaaaaccttc ttttggaacg gatgagtatt
     5281 cagttaagta tgctactaga attaaaggtg gatattacta tcattctgta ctatatgatt
     5341 caacaggaag ttatataata gatggcagac ttggtgaagc tcttagtcat ggatgtataa
     5401 gattaagtac ggaaaatgca aagtggatat atgataatat accagataca acaacagtta
     5461 taattcatta agaaaaattt tgagtttttt attgggataa taattcttaa attaatagaa
     5521 ttatatatta acttctcata aagagtacta tttaaaaagt gtaataagag agtgctatta
     5581 acaaaacaaa gatattttga ggcactctct tataaatata cataattaag atattttcat
     5641 aaacaattct ataatcttat ctttatcaaa gaaaacagga ttagcaccag cacaagcatc
     5701 tttcatagag ttttctgcaa gcaattcaaa attaggatta tcaacaccta catcttttaa
     5761 agattttggg attccaacct cttcagaaag agcttttatc ttatctatta caaagtcaac
     5821 acactcttta tctgatttat tatttacatc cattccaata acttcagcta ttggtcggaa
     5881 tttggcaggg gctgatttag cattttcctc ctcaactatt ggaagtagca tggcattaca
     5941 tactccatga ggtaaatcat ataaacctcc taattgatga gccatagcat gtacatttcc
     6001 aagacctgca ttactaaagg caattccatt taaaaaacaa gcataacaca tttgttctct
     6061 agcttctatg tcattaccat ttttaacagc tcttggaagg tatttaaata tctgctttat
     6121 agcatataag gcagttgaat ctgtaacatc ataagcacca ttagctacta cagcttctat
     6181 agcatgagtt agtgcatcca taccagttgc tgctgttaaa ttagatggtt tgctaatcat
     6241 taattctggg tcatttactg tcattgtggc tagtgaattg gtatcaacca ttatcatctt
     6301 tacatgtctg tcttcatcag ttataacata gtttattgta acttcagcag atgtaccagc
     6361 agtagtagtt atagctatga taggaagaca ttttttagaa gttttgttga taccttcata
     6421 gtctctaata tctcctccat tagtggctaa tatagagata gctttaccac agtcttgtgg
     6481 agaaccacct cctatagtta taacgaagtc gcaattttct gattttaata ttttaagtcc
     6541 tttattcaca ttagttacag ttggattttg tttaacttca tcataaacaa caaagtctac
     6601 atctatctga gataaaatat ctgttacttt ttttacagta ccatttgaaa ctagaaattt
     6661 atcactaaca ataaatgctt ttttagttcc taatgattta attgaatctt tagcttcatt
     6721 aagacatcct ttcccaagta aatttattgg tggtacataa taaatgctca taactaaaat
     6781 ctccttttat tttcattgat ttttatgtaa aaatatatat ctaacaattt tcactaactt
     6841 actcataata tatcgtatct tagattgaga taggattgtt ggttcttata taagaactta
     6901 attcgtaatt acgattatga ttagaattta acatagtatt tttatcatgt caataaaaat
     6961 ataaaaaata ttataaaaaa aacaaagtta ctgtagttaa atcagcaact ttgtttttat
     7021 agtataaatc aaataaatac attagtttaa aacaaaattt ttaattatta tgttttgata
     7081 tcttccatat acagaatatt aatctaattt attacaatgt cttcattacg ttcttccatc
     7141 attgattgaa tatttttctg agcatttaag agtaattttt ttatcagttc tattttttcg
     7201 tcattagctc taaataaagg cagtgttaca ctaacagcag ctcttataca tccatttttt
     7261 cttattggta cggcaagaca ttttaggtgt tccattgatt cctcacactc tgaagctatt
     7321 tgtgttcttt gaacttcaag aagctggtcg tataaaacat caaaatctgt aattgatttt
     7381 tctgtaaaac cttttagacc atctggatat aagcttatta gtttttcctt gtcaaaatca
     7441 cataaaagag cttttccaag acctgtacaa ttagcaggta ttttttttcc aacataagaa
     7501 ataagtctaa ttggttctgg agaatctact tttccaatat aaagaacttc attattgtca
     7561 agtataccaa gctgacaagt ttcagaacag gaattaacta tattagtcat ttcgtcaatt
     7621 atatagttaa aaatagttct tttttcaaaa aatgaagacc ctaatgtgta agaacgtatc
     7681 cctatagaat atttggatgt gtttttatca atagtaatgt attttctagc attaagtgta
     7741 tgaactatag gaaagatgct actttttgga gcattgataa catctgctat ttctgtaaga
     7801 gtatatccat tttcggattc tgctaatgct tctaaaatat ctaatatcct agctgttggg
     7861 cgatgattat ctgaagtcat aaatttcccc ctctttttat taatttaaag tattagaata
     7921 tgttgatttt agcacactat acgatatttt acaatgtaga aaataaatat gtaaatttat
     7981 atataaataa gctcttgatt tagatacaga tagatatttt tttagactgt acattaagga
     8041 ataactaatt tccaaaatgt acctaaagaa agtaagtgat atttagttat ataatttaag
     8101 taaatatatg caatagttat ttttatatgt aaatatcaaa atattatgaa cttatatttt
     8161 atctattttt tacactgtat aaaaacttta tagttttgaa tactatataa ttttaaatac
     8221 gctgtatttt tgcatataag gaagtcattt gataatatac aaggtattat cagaaagttt
     8281 gaaaaaattt tgacaaaaat ttattttttt tattgactca taaataggaa aatgctatca
     8341 ttaattcaaa gattcgtaat taaaaactta gttcttaaat acgaaccgaa acggccgtta
     8401 gaattaaagc taaaaactta ataagagtaa ttaaatatca tgttttagga ggattaactt
     8461 tatgataatg gattttatta aaaaaattcc tgcaggtatg atgatagtgc caatgtttat
     8521 agcagctttc attaatacct tttgcccaga tattgtacaa ataggttcgt ttacgacagc
     8581 agtattttca agtgttgggt ctgcaactat aattggggtt caactggtat gcttgggaac
     8641 tcagcttcaa tttagagaaa tgccaaaagt tttaaagaga ggtggtatac ttcttggttc
     8701 taaatttgct ataggtgctt tgataggtat acttatagga aagctatttg gaatggatgg
     8761 tgtccttggt cttacaacat tggctgttat aagtgcagtt acaaatagta atggtagtat
     8821 atatctttca ttgatgactt catatggaga tgaaacagat tgtgcaacta tgagtttact
     8881 tactttaaat gatggaccat tttttacatt aatagctctt gggacttcag gtcttgcaaa
     8941 tgtaccattg ttgtcattat tggctgcaat agtgccaata cttgttggta tgataatagg
     9001 aaattttgac aaaaaaatga aagagttttt agagccaggg tgtaatttac tcgtaccttt
     9061 tgtagggttt gcacttggta gtagcataaa tcttgcaagt atactaaaag gaggtttatc
     9121 aggagtatta cttggattaa tatctgtatt tgtaggaggt atatttatag tattctgtga
     9181 taaatttata ggaaaacgtc caggatatgc aggatgggct gtagctacaa ctgctggaaa
     9241 tgcaattgct gtacctgctg ctgttgcatt agttgaccca tcatggcaac catatgttgc
     9301 aacagctaca acacaagtgg cagccgcagt agttgtcact gcaatattag tacctcttat
     9361 aactagttgg tgggctaaga agtatggatg tcctaagttc ccaaaacaaa atgaggatgg
     9421 tacaataaca gcttaattta tattacagtg agttgttggt aaaataataa attctttaat
     9481 tagattttag gatattaata aaattatatt atttataaag gagagcaaga ataatgataa
     9541 atgcagtgat aattgatgaa aaagataatg ttgcagtagc tattgaagca atagctaaag
     9601 gtagtgaaat tagttttaaa ttaaaagaca aaacagtaaa aacgattaca gcactagatg
     9661 atataactat ttatcataaa ttggctataa aagatatggc ccaaggtgat aaagttacta
     9721 aatatggtga acatatagga gaagcaaatc aggagataaa aagtggtcaa catgtacata
     9781 ttcataatgt tagaagtgtt agagaggatt tggataaaca agttatgact aatatataaa
     9841 ataatattta tagagttata aaagttgtat tcattgattt tagtgtcaaa tagtttttaa
     9901 ataggttttt agctgtttga ttagtgagtg taataaaaat tataatatat aaaaattata
     9961 gaaagtatat agactatttt aggaggaaat cagtatgaaa ttttatggat ataaaagacc
    10021 agatggaaga gttggtataa gaaaccacat attaatattg cctacgagtg tgtgtgcatc
    10081 agatacaact agaataatag catcacaagt tcaaggggca gttaccttta ataatcagaa
    10141 tggatgttca caagtacatt cagaccaaca attaactatg gatgttatgg ctggttatgc
    10201 agcaaatcca aatgtatatg gtgtaatagt tgtatctttg ggatgcgaaa attgccaaaa
    10261 tgatttggta gttgatgcaa taaaagagag aacaaataag ccaataaaaa ctcttgtaat
    10321 acaagaagaa catggaactt taaaaactat agaaaaagct gttcgttatg ctagagaaat
    10381 ggcacaagaa gcttctttgc ttagaaaaga agaatttcca atatctgaac ttatactagg
    10441 aactgaatgt ggtgggtctg acccaacaag tggattagca gcaaatccat taataggtga
    10501 attaagtgat aaacttgtcg atttaggagc aacatcaatt ttaagtgaaa ctactgaatt
    10561 tataggtgca gagcatatac tagctaggag agcagtaaat gaagaagtaa aagaaaaaat
    10621 attacatata gtacatagat atgaaaattc attaaaattg gttggagaag aagttagaga
    10681 aggtaatcca tctcctggaa atatagctgg tggtttaaca agtttagaag aaaaatcact
    10741 tggttgtata cataaaggtg gtcatagaca aataagtgaa gtatatgatt atgccaaaca
    10801 aattgataag aaaggtcttg ttataatgga tacccctgga aatgatgctt catctgttgc
    10861 tggtatggta gcaggaggag cacaaatagt ggtatttagt acaggaagag gaacaccatc
    10921 aggaaatcca atagcaccag taataaaaat aactggaaat aaaataactt ttgcaaatat
    10981 ggaagataat ctggattttg atgctagtcc agttatatat ggtccacaaa caatggaaga
    11041 attaacagat gatttgttaa atatggttgt agatgtagca aatggaaaac aatcaaaagc
    11101 agagtccctt ggatatactg aaatggcaat agctagagta tgcaactatg tataatcttt
    11161 aagtaagaaa ataaatacat agaattttaa taaagggtga gattatgaag tttgtaagtt
    11221 ttactgaagg agaaaatgaa tgtaaaagaa ctggagtttt ttctaaagat gaattattta
    11281 ttatagatgt taactcttta aatctcagta gaaattttaa ggatttaaat gaattaattc
    11341 aaaatgtatc tagtgaagat attattaagt tgaaaactat tataaatgaa agtacattct
    11401 ataattatga tgtttttaaa agagagagat gtactgtaca tgttcctata gaaaaaccaa
    11461 tacatgatat cttatgtgtg ggagtgaact ataaagacca tcttgaagaa actcaaactc
    11521 actttgatga tagttttgaa gaaccacaaa aaacggttta ttttacaaaa cgtgtgacaa
    11581 aggcaatagg tccagaagat gagatagatg gtcattttga gataaataat caactagact
    11641 atgaagttga acttggagta gtaataggta aaacaggtgt taatattaga aaagaagagg
    11701 tagaagatta tatctttggt tacactgtta taaatgatat ttctgctaga aagttgcaaa
    11761 aagaacatgt tcaatggttt agaggaaaag gattagatac atttacctct ttaggtccat
    11821 gtattttaca taaaagtgca gttccttttc ccataaaact taaagtatgt agtagggtaa
    11881 atggtgaaga aagacaaagt tctaatacag ggttattcat atcagacgtt tcagacatag
    11941 tttcagaact ttcaaaaggt atgactttag aggctggaga cattatagct actggaacac
    12001 cttctggagt agggatggga ttttcacctc ctcgatatat gagtagtgga gatgtagttg
    12061 aatgtgagat tgagagtatt ggaattttaa aaaattacat taagtaactg ttttaatata
    12121 attaatatta aagctaaatc tgatgtatta atgaacatat taaaataaat tttaggagtt
    12181 taaattatga aagcagaatt tttgacacca gttgttacaa tatttgatga agaaggaaat
    12241 ttagataaag aaggtaataa aaaagtgtat gaccatctta tagatggagg tgtagatgga
    12301 ctagtcataa tgggaagtac tggagagttc tttaatatgt ctatggatat gcaaaaagaa
    12361 cttatagatt tagtaactag tcatgtaaat aagagagtaa aagtgtttat aggtacaagt
    12421 cgtatgtgta taaatgaatc tatagaactt tcaaactatg cacataatag tggagcagat
    12481 ggtgttatga taataagccc ttattatttc tctcttgatg atgatagtat tgagttattt
    12541 tatagtgaaa tagctaagaa tacagaggct gaaatatatt tgtacaatta tccagataga
    12601 actggttatg atttaagtcc agaacttaca ttaagacttg caagaaaaca taaaaacata
    12661 gttggatata aggatacagt aactttgatg ggacatacca gagatttaat aaatactatg
    12721 aaaatagagt tcccagaatt taaggtttat agtggttatg atgaaaactt tatacataat
    12781 ctaatgtctg ggggagcagg ttgtataggg gggttatcaa atttaattcc agaagtttgc
    12841 tccaaatggg ctgaagctta caataataga gatatggaga aggttataga aattcaaaaa
    12901 tatattaata aagcaatgga tttttactta ataaccaatc catttatacc agcaatgaaa
    12961 aaagctatga atatcagagg gttacaaata agtgattata tgactgctcc atttgtaaaa
    13021 ccaaatgaaa aacaagtatg tgaaattaaa aaattgatga atgaattaaa tatatgttaa
    13081 atcaaccttg caaagaggtc aacaataaat tacaattatc ttaataaaaa ttaaattaaa
    13141 ataatactag ggtactatat tagtttaata gggaaagtga tgaaaattca ctacagcccc
    13201 cgctactgta tacggatacg aaatcaaaat ccactgaaat ttataaaaat tttgggaagg
    13261 gtgatggagg aaaagccgtg agtcaggaga cctgcccagt attatataag ttcaatttag
    13321 ggtggagatt gtaatttata ataaaatgaa gtatcaattc gtttttgtaa agttgatata
    13381 tgaacttctc cttattaaat taggagaagt tttttatttt attaaaaggt tatacttatt
    13441 aaagatgact ttaggataac tttaataagt atactttaaa aagcatatat tattaaagtt
    13501 ttttagccac tcataaaaaa taagatattg gagtgaaaga gatgaaattt aaatcaaatg
    13561 ttttaaagca gtttttttta attgctatgg taatttttat ggtaggaggt cttatagggt
    13621 gtagtaaaag tgacaaagag aataaacaag ctagtactgg taagaaatta gaattaaaat
    13681 atgctagtgg atttagtgtt gaagatttag aagatggaat taaaaaagta actgatggag
    13741 ataatcgaga actgatttta atcccaaaga agattaaaat tcctgaaaaa tataaaaatg
    13801 ctaatatagt tagaacgcct gtggacaatg tattaatagc atctacaaca caggcttgtt
    13861 ctctaagaac aatagatgaa ctagatagta taaaagccgt aacaacacaa gagcagtact
    13921 ggactattaa agaaattaaa gatttaatga aagatggaaa aatacattac gttggttcaa
    13981 atttagcacc agattatgaa aaaatagtag acttaaatcc tgacttaact atagtgagta
    14041 gtggtttatc agaggctgat acaaaattta ttgacaaatt aaaagaatta gggcttaatt
    14101 atgttgtaga caatgagtat aaagagcaaa atccatttgg aagaatggag tggacaagtt
    14161 taatagctgc attttatgat aaagaagata tggctactag tgaactaaat aaaactgtac
    14221 aaaaggtaga agaagtaagt aaaaaaataa aaaacaaagc taaacctaaa gttgtatggg
    14281 catctgtata tgaaggaagt gcttatatag ctcctaaaga ttcatatgtg gataatatga
    14341 taaaaatggc tggtggaata aatgcatgtg cttcaataga ttcaaatgaa gggcgagtaa
    14401 gtattgaaca attgcatgaa gttgcaaaag atgcagatat atttgtatac tcatcaagtt
    14461 cagattatgc accaaattta gattctataa aatctagtgc acctgtattg gctgatttgg
    14521 atgttgtaaa aaacgggaac ctatgggtgt ttgctccaga ttattatcaa tcagtagata
    14581 agacagacga attaatagta gatttagttg agatgttcca cccaggtact acaggtgaaa
    14641 aaatcaaaca ttatataaat tatgataaat agtacagaga ggacacaaaa agataggttt
    14701 tatagatttt agttaatata gctttgctaa aaggagtata caaatggatt ttatcaataa
    14761 aatgagaata aggaatacga caatatttat agtattaatt ataagtatga tacttacttt
    14821 tttagtgtgt gtagcatttg gctctgtaaa aattgaattt aaagaagtaa tagatgttgt
    14881 attaagaggt atggataata cagaacatta taaaataata tacaatataa gaatccctag
    14941 agtacttgca accatggtag gtggagcatg cctagcagta tcaggtattt tattacagat
    15001 attttttaaa aacccaatag ttgaaccgta tatattagga gtatcttcag gttctacttt
    15061 atcagtagct ctcatgattt tgggaggata cacattggga tttgagagtg taaattcaat
    15121 gagtatattt gtagcagctt ttataggagc tttaattgta atgtctatag tcattttatt
    15181 tgcaaataag gtaaagaata taacaactct tttaataata ggaattatga ccggatatgt
    15241 atgtagtggt ataacaaatg tgttacaatc atttgcaaat agtgataaat taaaagaatt
    15301 tactatgtgg tctatgggaa gtttttctgg atttacatgg gaaaaggtag aaatacttat
    15361 aatagttggt atattaggtc ttttgatgac tgtattcata ataaaacctt taaatgcttt
    15421 tttactagga gaagagtatg caaaaagtat tggcataaat ataagatttt ttagagtttt
    15481 gatagtattt atatctagta tattagcagc aatagtatca gcatttgcag gacctatagg
    15541 gtttattgga cttgcagtgc cacaaatttc aaaaatgata tttaaaacat cagataatag
    15601 agttttgata ccagtagtaa tatttttagg cagtatagtt acagctcttt gtgatttaat
    15661 atcaagagta attttatcac cagtagaatt accaataagt acaataacat ctttattagg
    15721 agctccaatt gtagtaatgt tattggtgaa aaggaaggat agggcttaga catgttgaaa
    15781 actaataatt taagtgtagg atacaataac aaagttgtta taagtaatat aaatgtagaa
    15841 gtaaaaaatg gagaaatact atgtcttttg ggaagtaatg gagctggaaa aactacccta
    15901 ttaaggtcct tatctaaact tatatcacca ataaaaggtg aaatatattt gaatggtgta
    15961 aacataaatt gtatatcaag aaaagcatta tcaaaaaaaa tggctttagt acttacaaat
    16021 agattattag gtgatttaat gactgtacaa gatatagtca atataggaag gtatccatat
    16081 acaggatttt ttgggagtct atctaagaaa gatttaataa tggtagatga agcattggag
    16141 tctgtagatg ccttacattt aaagaaaaga tattttgatg aactaagtga tggagaaaaa
    16201 caaaaggtgt tggttgcaag ggctcttgtt caagaaccag aaataattat attagatgag
    16261 cctacaactc acttggatat aaaacataga ttggaactca taaacatact gaaaaaatta
    16321 agtaaagaaa agtcaatatc agtaatactt tcattgcatg aaatagacat agcattaaaa
    16381 agttgtgata aagttgctct tataaaaaat aataaagtca tagcttatgg ccagcctgaa
    16441 gatgtagtag atgaggatat tataaattcg ctttatgaat tggatgataa aaactttaat
    16501 agtcttttag gttcagtaga aattagtaat aaatctaaaa atgaagtttt tataattggt
    16561 ggtgggggta aagcaacacc aatttataga gcttttacta aaaaaggtat aggtctgtat
    16621 tctggaataa tacatgaaaa tgatattgat tatgaaattg gtagaactat ggggataaag
    16681 atgtttacag aaaatccatt tgagcctata agtgatgaga gtttcgattt ggctattaga
    16741 aatttaaatg attccaagat tataatagat acaggattta gtgtaggaga gacaaataag
    16801 aggaatatag atattataaa agaagcttta aaattggata aaaaagttta ttcttttaga
    16861 aatagagatg agagtaagaa gtattatgac agtttggata gtaagataga acatatagat
    16921 aaagtttctc aaataatcaa tagtgcagat ataaataatt tattataatt tatgaaataa
    16981 aatgtactta tgtgttatag aaaaaagagt atagattata atttttttga aattacaatc
    17041 tatactcttt tgttattttg tttatgtatt tatatattta taaaacttta gcattttata
    17101 gataaccaat agcttcctct ctagttaaaa aaaagtgaat ccctgtagtc gattctttcc
    17161 atctattttc attaaaatct tcagctacaa tcgtttctcc tactctatat ataaaatttt
    17221 catcagcata tgcacgagca cttttaaaag attctttacc atctacacta gtaattgaca
    17281 gtacttttgc cttatcacat ctacaagcat ttgatgtagc agacactctt tttgcgtcgc
    17341 tgggtattaa taattgtacc attctaaaat taaaacattt tttatagcct ataaatgctc
    17401 ctgtttcagg acaatacaat ttaaaaaaac gagtactttc atctgatatt actccattta
    17461 aagtagcata ttctaaattg gaattttcta aattagctcc tctaatatct gagttagata
    17521 agtcacaaaa tctaaaactt gcaccactta agtctgcatt ttttaatata gaatttttta
    17581 atgaacaatc cgaaagataa gaattattaa aaatagagtt ttctaaatct gctttttcta
    17641 aatttaaatt gataaggtca gaccacgaaa agtcaatatt acttaagcag tatcctctta
    17701 agtccatatt ttctaatctt aattctttta aatctgcctt ttctccagca ttattgtttt
    17761 taagccataa ataatgtttc tcaagtgttt tttctaattc ttgtttagaa atagt
//
LOCUS       DAYATN010000069        17799 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.069, whole genome shotgun sequence.
ACCESSION   DAYATN010000069 DAYATN010000000
VERSION     DAYATN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.069"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(584..1318)
                     /locus_tag="ACGI8A_002262"
     CDS             complement(584..1318)
                     /locus_tag="ACGI8A_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0180258.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(2329..2967)
                     /locus_tag="ACGI8A_002263"
     CDS             complement(2329..2967)
                     /locus_tag="ACGI8A_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFH0180259.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFTLWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(2970..3827)
                     /locus_tag="ACGI8A_002264"
     CDS             complement(2970..3827)
                     /locus_tag="ACGI8A_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180260.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(3829..4203)
                     /locus_tag="ACGI8A_002265"
     CDS             complement(3829..4203)
                     /locus_tag="ACGI8A_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0180261.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(5365..6555)
                     /locus_tag="ACGI8A_002266"
     CDS             complement(5365..6555)
                     /locus_tag="ACGI8A_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFH0180262.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(6571..8526)
                     /locus_tag="ACGI8A_002267"
     CDS             complement(6571..8526)
                     /locus_tag="ACGI8A_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFH0180263.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(8543..10588)
                     /locus_tag="ACGI8A_002268"
     CDS             complement(8543..10588)
                     /locus_tag="ACGI8A_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFH0180264.1"
                     /translation="MKKITKERIENTMKKLFTSETFLEYQFISDCQISPDGGYTAFIV
                     KKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRNEKVKEAV
                     KNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRPIVEGLPE
                     EEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAITAPMFNV
                     VGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGFELYQNQV
                     ILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGAGQTTKVD
                     GEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCLGNGLPEV
                     YTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYEVGKKYPA
                     VLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQFGEIDYI
                     DFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRSIANWSGM
                     EGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYVDKCVTPTLFLHGEKDYRCYMQEA
                     FQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIKKDNIGQ"
     gene            complement(10975..11625)
                     /locus_tag="ACGI8A_002269"
     CDS             complement(10975..11625)
                     /locus_tag="ACGI8A_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0180265.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            complement(12609..12941)
                     /locus_tag="ACGI8A_002270"
     CDS             complement(12609..12941)
                     /locus_tag="ACGI8A_002270"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFH0180266.1"
                     /translation="MAQPFEVEPKLDELIEWYYSQSEVSIKVITEFHYRFELIHPFQD
                     GNGHIGRFVMLKQMLENNLPIKIVSWDSEELYRNSLNSCSLGNYVPLIEYLSSLEDFR
                     EVYKMLWK"
     gene            complement(13042..13722)
                     /locus_tag="ACGI8A_002271"
     CDS             complement(13042..13722)
                     /locus_tag="ACGI8A_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFH0180267.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(13719..14645)
                     /locus_tag="ACGI8A_002272"
     CDS             complement(13719..14645)
                     /locus_tag="ACGI8A_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180268.1"
                     /translation="MNYIIETNQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(14718..15635)
                     /locus_tag="ACGI8A_002273"
     CDS             complement(14718..15635)
                     /locus_tag="ACGI8A_002273"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0180269.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAMLQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(15635..16324)
                     /locus_tag="ACGI8A_002274"
     CDS             complement(15635..16324)
                     /locus_tag="ACGI8A_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0180270.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(16732..17037)
                     /locus_tag="ACGI8A_002275"
     CDS             complement(16732..17037)
                     /locus_tag="ACGI8A_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180271.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
BASE COUNT         6068 a         2962 c         1929 g         6840 t
ORIGIN      
        1 atggtttaaa taatcttctt tactttgtat tatgatacat gctttaaata tttttacgca
       61 ctttatatta taatacatgc tttaaataat cttattcgct ttgtattata acacattatt
      121 taaatatttt tactcactct gcattatagt acattattta aatatttttc ttcgctttgc
      181 attatgatac attatttaaa tatttttact caccttgtat tatggtacat tatctaaata
      241 tttttcttca ctctgtatta tgatatataa cttaaatatt ttacctcact ctacaatatt
      301 atatataatt taaatatttt tcttcacttt gtatactaaa gtttatttaa agtattttct
      361 ttcccattat actatgctgt taaatatttt tcaactgaag tattgtagaa ccttaaaatt
      421 actataaaaa actatattct aaaatacttg taatactacg tatcaccctt aacacttaag
      481 tataatttta aagcaaaatt cttattactt atatttaatt ttgattctac atctttagat
      541 ataattttaa gtaatttaag aaattattgt aaatcatata gtactactca ataattaggt
      601 ttttcaatgt tccaattatg tttctttgat ataatccacc atgactaaaa actatcgttt
      661 ctatatcata attacttata tttttaatag catcttttaa ctcttgtttg ttgctgtgca
      721 ttacatcaac aggcttaagt tctccatttt ctacctgtaa caaatctcca gcaataagta
      781 ctttagactc ttttaaataa agacatatgt gacctctagt gtgacctcct gtatgtataa
      841 cttctatttc ctcacctaga ttcaattttt catggttatc taatgacaca tctactttag
      901 tatatgatga tttaaatcca agtcccataa catttagcat actaagcatc ttatcatcaa
      961 ttttatctac catttgttcc aacatataaa gtttaaaagg agtttcttct cctgttatat
     1021 agctgatttc acttttgtat gcatatactt tgatattatt cttagatttt tctcttagat
     1081 aatttatgtt tcctatatgg tctatatcat gatgagtaat tattatattt tttaacttat
     1141 caaagctcgt gttactttta ttaagagcat caattattaa atcaccttga cccggaagac
     1201 ctgtgtcaat aagtgttgca tcatcttctg tgtagattac aggaatatac ataactttat
     1261 cttcaccgaa tacatttgat gaaattttta aaacatctaa tccctttgct aaattcataa
     1321 tttttctcct tataattact taattagagg tcaatttaat taaagaaatt gagaaaatgt
     1381 agattttcct ctagttatat tttattaatt aaaaattatt aatacctagt atatcactta
     1441 agttgaagga ttactcgact tgattttttt gttattttat ggtatagtaa ttaatataga
     1501 agtaagtaat tgcaagcttt gcaattactt ttattttttt ctaataacta ttttattttt
     1561 gataattcgt ataaaattaa aagtaaagta atatggaaaa tatgaatata gaaaaacttt
     1621 aataagaaaa taaattatga ataaataaaa gaaacgcaat actgtatata gatgattatt
     1681 tagaaaaata aaatctcttt taataaaaat aagcttcaaa ataaactttt agttcataaa
     1741 gcttaggagg aactaaattg agtgcaagtg tatctactgt tcaaaataaa tagttcataa
     1801 agcactcttt tttatattaa atcaattata ttttcgtcat ttcagcaatg aaaaagaaac
     1861 ttatctctat tttgagacag cttcttttta ttaacttcct actttttatt agttttctaa
     1921 aattgaaaag ctttatttat aggattcctt agatttatat tttgacgata taatttttta
     1981 aaaacatctt aaaagatggt atacaataca cacaatcata ctcataattg aaaaataatc
     2041 aatggaaaat aaaattactg gatattatct attaaaaaat acaaattgat tcacaagaaa
     2101 catataatct ataatattat gatgttactc tctcgcacca agtaacttta ttttcaagta
     2161 accttttaaa ttcataccat ctaacttcac tcaataaaaa tggcaatata aatatgtact
     2221 ctatttttta cttttaaata tctgacaagc tttatatact attggaagta aaatacaaag
     2281 taaaacagcc caatctggta aatgtggcat ataaaagtcc cccttacctt atacaagctc
     2341 cttattacta aaaattttaa tagaaataaa cattgaagct aacagtataa ttaaacttaa
     2401 aaggaacaat acaatattca ctatcatgga tggatactga tatataaatg gaatttcaaa
     2461 attgctgtga gctatagtta atggtaatac aaatgatgaa gccatgatta tcaccacgaa
     2521 aaagaatttt gttgtttcat atcctaattt atattgaata ggcatataaa ctccataaat
     2581 aatagaactt attaataaaa caatcaaaat atatgttaag tcaaaagttc ctatttgtgg
     2641 aataagtaaa ttttcaatcc agtaaactaa tgtacaatag ataaatacca ttgcaaataa
     2701 aacatactta gattgaacta agccacgtct aggataagga gttgaacaaa gtaatacaga
     2761 tgcttttgaa tattgactct cctttaataa tagatactgg cacagcataa attctgaaaa
     2821 tataatggat agtacaaatc ccaatatttg tgcaaatgga ggtaaacgcc acaatgtaaa
     2881 taatgggatt cctattgcaa taaacaatat caacaataca tattttttta caatcagaat
     2941 atctttttta actaaatgaa tcaacataat tactctctcc tttcaatata tgcaagcata
     3001 atatcttcaa ttgtaggacg ctctattaaa atatctggca tatttttttg cacttcatta
     3061 atttttgagg taatccctat aaaaccataa cctgtaattt ctaaatttaa aaataatttt
     3121 ttgatttcat catttagaaa ttgaatatcc ccttttactt ttctatgttc gtccaataga
     3181 gtatcttttt cacgctctaa tataatacgt ccattgtcaa tcataacaat catatctgct
     3241 attttgtcta aatcagatgt tatatgagtg gaaaagaaaa caccacgacc acattttttc
     3301 atatatcctg ttagtatgtc aagtatctga ctacggatta gtgggtctaa accacttgta
     3361 ggttcatcca taattaaaaa ctcagcttta tgcgataatg ctaatgctaa tgcaaatttc
     3421 attttcattc cttttgaaag tgtatcaatc ttttgttttg ggttaagatt aaaacgcttc
     3481 atattttgtt gataatcttc ttcacaccaa ttatcatatg aaggtgcaat aatacttttc
     3541 atttctgcca ttgacaactc ctcatagaaa tatgaatcat caaatacaac tccaattcga
     3601 ttttttaatt ccttctcatt tccatcgaaa tctttaccaa agatttttat ttttccacta
     3661 tcacgcttta cgacatccaa tatcgaacgg attgttgttg tttttcctgc tccattaaca
     3721 cctataaatc cagttataca atcttctggt aatgcaaagc ttatattaga taatgaaaaa
     3781 tcggtataag tcttatttaa gttataaact tctaaaatat tttccatatt aatcctcctg
     3841 atatagaata tccatcattt cttgtaactc tatctgtgta attcctagtg cttttgcatc
     3901 cttaatcata tctgccattt tttgttcaac tatacgtctc tttgagtctc ttagtatttc
     3961 taaatttcca gttgaaataa atgttcctat tccaacttgg cgtgtaagaa agccttcatt
     4021 ttcaagttct tcatacactc gtctaatagt caaaatacta acttttaaat cattagcaaa
     4081 atttcttatg gatggcattg catccccttc tttcagttct tcagtaaata ttgcttcttt
     4141 aatttgctct ttaatttgtt ggtataatgg cttatctgat gtgttagaaa tgagcacttt
     4201 cattttttat cacccccttt ttatcatttt cagtgtgatg ttgttatata cacactatac
     4261 acttagagca cttttttgtc aatataaaag atgtaaaata ttactatgac aaaataaata
     4321 tgaatgaaat attagagatt gttgatagct ataaacactt tttaaaacca cataagatag
     4381 aatgtattaa atatttatta aaagtaagaa ttgaatatct aaaaaaaaga ggaatcctgt
     4441 atgaatgatt tgtttgatga taaattaagt tataaactat ttataaaaaa cataaaggaa
     4501 taaatccatt tctaattaca aacatactaa aaaatttaga tagtaataaa tagatagtaa
     4561 taaatagata gtaataaatg ggatgaattt ataagaattt ggatagagga aagagttatt
     4621 caagagtgta gactagaggt aaactctata ttaaaaaaat ttaaatttgg atattatgat
     4681 gcatggaata tttgcagaag taaccttgct ataagtatag aagatttttt tgattacaaa
     4741 agataatact ctaaaatgtg aggatttcaa cattcaatac tgtactttaa ataatatcac
     4801 tgcaaaatac catccacctt ttaccataat tgaataccct aattatttta aaaaagaaaa
     4861 cattagaaac agaaaacctt ggatatataa aataaaaaat actaacttct aaaacacgga
     4921 tactctttga taaataaaac aaaaaataat tttaataatt aaaattggct atctcaaact
     4981 agagataagt ctctttctca cttttaaaac gttgaaaata tattcgattt cgtacaaaaa
     5041 agagtgcctt agaactaaaa agttcacttt gtggcactcc cttttttctc ctctttttaa
     5101 ttttttataa aaattcttca aaaatattat taaataccct atattataca taaatcaaat
     5161 taaagttgca ttttagcaaa taaaaacaaa ttataactat tagcatagca aataaaaaat
     5221 ctgctctctt ataaaatcaa attataatta taaaaagaaa atattcttat aacaaattag
     5281 ctaatataaa attattttat aaattaatac tactttttag cttctcctat aaattaatac
     5341 cactttttta actttgaaac ctatctacaa cattggtacc ataagaccag ctaccacaat
     5401 agaaatcata caagccatac caccaactac cagcttaggc ataatcttag acatcaaata
     5461 tgctctttct tcttccgttt ctccaacagc attacacact tcatttgaaa ttaaatatgt
     5521 agttgggaat cccaacatct gacaaaaccc tactccaaaa gcaagaggtt tagaaccgat
     5581 aattttccaa cctggtaaca ccttcatcat aaggaatata ccaatgatag aagctgcaaa
     5641 catacccaca attggtacaa aaagactaat taaatctgtc aaagaaactt ttgctaaaga
     5701 aggaataaca gctgcaaaca caaccatgtt gataaatccc ttgatttttc ctttttccaa
     5761 aatgtctttt ggcacaatac caatacgtgt tacaataaca ccaacaacaa gacatacaat
     5821 agaataattt acatgaataa tctctgaaag ttcataccct agataagaga ataatgctat
     5881 aaaaaatata caaacatttg tagaataata tttatcgtat ttttcacaga aagcttgttt
     5941 tacttttgtg cctgtattct ctgcttcttt cttatcagta tcaacatttg ctaaatcaat
     6001 gccttttgat tttgcttcat gatattctac taaaagacgg tttgcctctt gtaacgctgc
     6061 tcttgaagct attggtgtac caataaactt ctgaattgca aatacaactg ttgctgtagc
     6121 tgctgcaagt gtcagtcctt tttctagtgc tgcttgtgtc ataatagtag tagcaggtaa
     6181 agctccattt ataactggta tgcttgtcaa tactgttgat tttccaaaca aaggaataca
     6241 taatgagaca cagactataa ctgctacaat tccaagccat gctgtcaata ctgttctcca
     6301 ttcgtctata aattgtttaa cattcaacat tgttcccata ctaaacataa tcattggaac
     6361 actccaacta gctgctgcgg tcatacctgc tttttcaata atatcagctg gtattgcttt
     6421 tgtgacaaat agaatcaaaa ataaaagtag tgtaacaaat acagctgata cttttgcttt
     6481 tgttttgctt gcaattaaat ccccaatagc aaataaaact aatacgacac taaaccataa
     6541 taacatatca ctcatagaat aaccccctta ttataaatat ctttcaaacc aatctacaat
     6601 agcatttgct cttgcaattc tattagatgg tcttccattt ctagacaatc catgtgtatc
     6661 tccgtggaac aaatacattt ctgtatctac accaagctgt tttaatgctg taaacatttg
     6721 gaaagtatcc cccataaaac atctataatc cctatcacta tgtaagaaca atgttggtgt
     6781 cttaacattt ttcacatatt taataggtga atgattccaa agaacctcct catgattcca
     6841 aggcgtctta tctccacaag tctgcttcat aattctatcc ccaaagtcaa taattaaaga
     6901 agctgtaatc aagttactaa tacctctttg agaaactgct gctttgaaac ggtctgtatg
     6961 accaattatc cagtttgtca tataaccacc gtaacttccc cctgcaaccc ctaagcggtc
     7021 tgcatcaatc ccaggatatt tttcaataca agtatctaca aattccataa gatgctcata
     7081 gtcaacatac ccccatcttt ctaccatttc taagaatttt tcaccataac cgtcaccacc
     7141 atgagggtta caataaatca catacattcc ctttgatgtt acacactgct tcaagtgtgt
     7201 aaacatatag ccatatgccc atttaggccc accatgaatg aacaatacag caggatattt
     7261 ttcgccttct ttagcatcta taggtggtat aacaaaacca tcaacttctt ctttattttt
     7321 tgctacaaac gtcaattttt caggtaatga aaccttatga gtatctaaat atggtttaga
     7381 aaaagcagtc atttttttca ttgtacctgt agtcatatca atgctatata tttctgctaa
     7441 ctcataatct ttcaacatta ctgtgtacat ggtgttgtcg taaatatcaa aagatgtaaa
     7501 agcatctact tttgtgatga tacgtatatt acctgtcttt gtatcaaact cattaaattc
     7561 tgtcatagtt ctgcaaattc ttgcaccaat aaatttatct cctataactt tattactacg
     7621 tccaccacca tatactgcat ctacatccag taatgtagca tacataccat ctggactagc
     7681 aatcatattt atttcactag tactcaaatc aagtataaaa atatcatgat tctgtgtaat
     7741 agaacggtct aatgttgaag cttgcaacat tactttatct ttgccaatat attttgccaa
     7801 acttatttgg tactttcctt gtggtacaag ttctttggtt tcaccactgt ctatatgata
     7861 ctcccaaagt ccagaaaaag gagttgcaca atcttcataa atctgaccag tatataaaat
     7921 tctgttttta tccgaagaaa catctatacc tgctacttca caaaattttg gtgtaatggc
     7981 ttttgttata ttttcacctt cttgatataa ttctaatgtt acacgtcttc tttgagaaaa
     8041 attttctcca tctcttataa aaggtttatc tgtaaatgtc caataatcta caccctctac
     8101 tgccctatct ggttctgatt ttttagaatc cgttggattt tttgttgtca gtaaccacaa
     8161 tttatttcca agtgatacag gtacgctaca agcttttgga atagaaaatg ctttttttgc
     8221 ttctcctcct tctatagaaa tagtgtaaaa atctgttgta cctgcttttg gcttatttct
     8281 tttagatgta aaaagtagtt ctgtattaga aatccaacta aatgatgtta catcttttgt
     8341 ttgagtcata tgtatttgtt tttctgtttt gatgtccatc acatataagt catgttcata
     8401 gcagtctttt tcctcgttga tatttgatat aatataagca atatgctttc catcaggtga
     8461 acaagaaata tcatgaggga atatatattc aaaaatatct gaactttcaa tctttatttt
     8521 ttccattttt atattctcct ttctactgtc ctatgttatc tttttttata tataccgaaa
     8581 accaatctag catttcaact aatctttgta gcttttgttt tggtctacca gaacgagaca
     8641 gttcatggtt ttctccttca aatagacaaa gttttgtagg acacccatga attttcaatg
     8701 ctgaaaacat ctggaatgct tcttgcatat agcatctata gtctttttca ccatgtaaaa
     8761 ataaagtcgg tgtaacacat ttatcaacat aagcaagagg tgactgtttc cattgaagct
     8821 cgtggttttc catatgagaa gcacctgttt gtcctttgca gaaataataa ccaatgtctg
     8881 ttgtaccctc catgccactc cagtttgcaa tagaacgctg agcacaagca acagcaaaac
     8941 ggtttgtatg accaattatc caattcgtca taaatccacc ataacttcca ccttcaacag
     9001 ccaattttgt tttatcaatg tctgtatatt tttcaagtac agtatctgta aattccataa
     9061 aatctatata atcaatttct ccaaactgtt tcctaatatc tgcaaaagca ttgccacgac
     9121 cttcagaccc ccttgggtta cagaagaata caaagtaacc tgcacttgta aatacctgta
     9181 tctcatgcat aaaatatggt ccatatgtca atcttggtcc tccatgaatc gccaatacag
     9241 ctggatattt tttccccacc tcataatcta ctggtttata tacgtatcct tgtatttccc
     9301 atttagaact tccttttgac tctatgtatt ctggcacaga aattttatat tcctctaaaa
     9361 tatggtcatt taaatgtgtt ttcttacaca atgttccatt ctctactgta taaacttctg
     9421 gtaatccatt tcctaaacaa cccacacata caatattgcc atcttttaca tctataaaat
     9481 caactgaacc tgtctgtgtt actttttcct gttttcctgt tggaatatgt attctttcaa
     9541 taatgcagtc catatctact gtagaagtgt aataaatata ttctccatct acctttgttg
     9601 tctgaccagc acccatttta acatcagatg ttactgtatt tccaatacaa tgatgttgat
     9661 gccctgaaag aagtttcatt tctttcgtat caatatcaat agtataaaaa tctccatttt
     9721 ctccatttcc ataagataaa gcatcagtta ggtttagtat cacttgattt tgatataatt
     9781 caaaaccttt tataatatac ttatcttttt ctaatataca gacatttgtg tttgtgtttg
     9841 tatcaaatac atagacccca tttttcaaac cttttacatc tttaaattct tgccctatat
     9901 ataagatttt tccatcactt attttcatac ctacaacatt aaacataggt gctgtaattg
     9961 ccttcaactc atttatatta ctatcgtata catataggcg ttttcttttt ctgctaatat
    10021 atccctcccc attgacagca taaggaattt ctttgaaatg ttcataatgt cttttgttat
    10081 aagcatttat ttttttctgt tgttcttctt ctggcaatcc ctctacaata ggtcggtcat
    10141 tgtcataaga atcaataact gcatacaaac cttttcctat aggattcaat ttaccacctt
    10201 tgatagggag tctaaaaagt tcttctgctt ctccaccatt taaagataaa gcatagtaac
    10261 tcatgcattg cttaccgttt ttgacagctt cttttacttt ttcatttcgc aaagcaggga
    10321 acaagatagt attttcatct tcccaggcat atgctccaac agagtttata gacgttattt
    10381 gttttaattc gccagtttta ttgtttaata tataaacttg gcttgtgtaa ccattctctt
    10441 tgatatcagc tttttttaca ataaatgctg tatatcctcc atctggtgat atttggcaat
    10501 cacttataaa ttgatattcc aaaaatgttt cactcgtaaa tagctttttc atagtatttt
    10561 ctatcctttc tttagtaatt tttttcaaac tccctaccac ctctttcatt ttttatttta
    10621 aatttaaaat aaaaaatttt aaatttaaaa taaaaaattt tataaaggtt atttttttag
    10681 gaagccttct ggcttccttg taatatttgt aatttaaagt atatttaatc catgcttaat
    10741 tcatgcttaa ttcatactta atctatactt aatctatact tagtccatac ttaatctata
    10801 tttaattcat acttagactt atttacacta tatttagatt atatttatta gattatattt
    10861 attagattat atttattaga ttatatttag attatgttta aactataatt agattatact
    10921 tagactacat ttagcaactt aatagataag tatatatagt aacttcaaaa cttattattc
    10981 atcttcacag aatcttttaa tagttccttg aatatgattt cttagtttat ctactgcttt
    11041 ttttgtatct ctgtcttcaa tgaattttaa tatttcaaaa tggtcttgta ctgttccttc
    11101 ataattacga ttttttaatg gtcgaattaa atagttcact actataaatc ttccttgtat
    11161 accaacatat acatcattta aaatctcatt atttgcccct tctacaatgg ctttatgtaa
    11221 ttccaaatct gctagtgcaa gttctacaga tttttcttga cgatagtact cctcgcattt
    11281 atcagagtat ctttttatta catctagctt ttgttgaaaa acctcttcct cttcttgcat
    11341 cattttttct aaagcatatg tttcaatcag tgttctcgtt tcacaaactt gttttaccat
    11401 atcatattca tactctggta cgtacacttt accatttgtc ataatagtaa gcactccatc
    11461 tttatttagc atttttactg cttgtacaac tggtgttctg cttacttcaa atttttcaca
    11521 aaggtcattt agtaatattt tttgacctac agagtattca ccacgaataa gactttcaat
    11581 aatcttatga tataaaattt catctaaatt tgactttact ttcactactc tcaccttatt
    11641 tctattcttt tgccatatta tattgaatta ctggacttta attactttta gtaatatttt
    11701 taaagtagaa attcttattt gatagtactt ttgtactaca atttaaatta ttgtactgta
    11761 aatcatgctc ttatactata gattaattca ttgtattgca aatcaagtta ttgtactata
    11821 gattaaatcg ttatacttca aattaaacta ttatactata gattaaacca ttgtacttca
    11881 aattaaacta ttatactata gattaaatcg ttgtacttca aattaaacta ttatactata
    11941 gattaattca ttgtacttca aattaaacta ttataccata gattaattca ttgtattgca
    12001 aatcaagtta ttgtactata gattaaatcg ttgtacttca aatcaagtta ttgtactata
    12061 gattaaatcg ttgtacttta aattaaacta ttatactata gattaattca ttgttctgca
    12121 aatcatgcta gtgtactaca aattaaacta taaatgttta ctatctctta ataatttgaa
    12181 taatatgtta caaaaagtat agcacctttt tatttttttc gcaataaata aaaaatttaa
    12241 aattttttat tttttatttt cttatattat caatttgaaa tatgaaagat taagactaat
    12301 agaattgatt cagttaattg aaaaaaatag gtacctttta tgaaaattct aatgataagc
    12361 atttttctga tgtaatggta aattataaat gataaaatta aaaatcactt ttagttttat
    12421 gaactgtccc catattaaga aacaactaag atacataaaa atagttataa agagatagtt
    12481 aaactatatc cactatttta tatagttaaa atagtgggta tagtccaagc taagaataac
    12541 taagaaatta gcgttaaata gcatagataa aaatacattt atatataaac tttttttgct
    12601 gcaaacaatt atttccataa cattttatat acttctctaa aatcttctaa acttgataaa
    12661 tattcaatta aagggacata attacctaaa ctacaagaat ttaaggagtt tctatacaat
    12721 tcctcactat cccaagatac aatttttata ggaagattat tttctaacat ctgttttagc
    12781 ataacaaatc tacctatgtg accattacca tcttgaaatg gatgtatcaa ttcaaatcta
    12841 taatgaaatt cagttataac tttaatagat acttcactct gactatagta ccattcaatt
    12901 aattcatcaa gctttggctc aacttcaaat ggttgtgcaa tattattaaa actctaataa
    12961 tattaataat agctgatata atagcaaatc attattatcc cgagaagata caattaataa
    13021 ctaccttcct atccacagta tctagtcaat ttcagtaact ttatgattta gtatataata
    13081 tataccaatc acagttatac caactataat aaaaacagaa atcaaactgt cttgagttgt
    13141 atttgtacca gctaaaataa attgtcttaa attgataaaa ataattgaac tgacaagggt
    13201 aacaggtatt gattttctta tcattccaac aacgaatgtc catataccta atgagcaaaa
    13261 tacaataata cttttaattg ctcctggtat ccaataagat aaaattgaaa tatgaaaatc
    13321 tccaataact aaattaaaat acttatccaa cacaacaacg aatgtaaaac aacagatata
    13381 tccaactatc attgatgata tcataagagt cgtaatcaaa agtaactttg ataaaataag
    13441 ttttttacgg tcaagtggat aagagaataa aatcaagatt gttttatttg tatattcatt
    13501 tattatatat gccacattca acacagcaaa taatataata tagtaaaaac agaaaaataa
    13561 actgattgtc ctaaatgtaa catcataatc atatattgaa gtagatttat cattcaatcc
    13621 agtaaagaca aaaaacatac ataagaaaat ccccagcatt gaaaataaaa aatatctttt
    13681 tagcccaact ttttttagct ctagtttcat taaatgtatc atagtgtttc tttacctcct
    13741 gtacctgtag tttggaagaa gtaatcttct aaagaacttt gtttacgtcc aatgctattt
    13801 ataccaattt gattttgaat aaatattttg gatatttctt ttacatcttt tcgtaaatca
    13861 tatatttcaa tacaactttc cgaagtaatt ttaaagtctt ttattccaaa ttctttttct
    13921 aacaagtatc cagcaagctc cacattatct acatctactt caacataatc tgtttgatat
    13981 ttgtgtatat ctgacataga aatttcttta ataagtttac catcttgaat aattccaata
    14041 gtgtccgcaa tttgttctac ctctgaaaga atatgactag aaataaatat agttattcca
    14101 taatctgtat ttaacttacg gaataattct cgcatttctc ttatcccctc tgggtcgagt
    14161 gcatttatag gttcatcaag aatcaataat tctggtttag taagaattgc tctagctatt
    14221 gcaagacgtt gtttcatccc taaagaatat tttgaaactt tcttgtttga ttgctttgac
    14281 aatccgacac attccaatac ctctgatata tgttctttgt tataataacc catatattca
    14341 cagtgtaaat caagattctg tttagcagtc aaattctcat ataaatacgg attttcaata
    14401 atagtaccaa tcttttttaa tatttcaaaa tcagtttctg ccactttctt accgaacata
    14461 actatttctc cagaatttgg cttaacaaga tttagcaaca ttttcattac tgtactttta
    14521 cctgccccgt ttggtccaac aaatccataa atttctccct ttttcacatg aatattaaca
    14581 gcattaacaa cagctttatc tttatatatt ttctttaatt gattggtctc tatgatatag
    14641 ttcatcatac tccctccttt ctaaaaacag tatacaaaat aaaattatct tttctctctt
    14701 ttaaatctaa cttttttcta acttttcaat tttactgtaa aaactgtttt tacattaggt
    14761 ctactattta cataaattga acctcccata gagtttacaa gcccttgaga aatagccaaa
    14821 cctaaaccat tgcctttttt tctgtcagca gtataagctc ttgtgaaaat atgctctttt
    14881 tcagtttcag aaatacctga acctttatcc tcaatatcta taaaaacatg tttttccttt
    14941 tcatatagtg aaatccctaa aaatctacca tctgaaccat attttatagc attatcaatt
    15001 aaatttttga gtatacgttg taatgcttct gtgtcaacat ttgcatatat aggtctatct
    15061 tcaatattaa cttcaacatt aaagttttct ttatctagca aatcataaaa ctctaaaata
    15121 atctcatgac ataattgtgt tagattggat ttttgaagat ttaatatcat atctccagat
    15181 tggatttttg ccatattaaa caggttattt attgaattaa caagctcatt tgaatttgtt
    15241 tgcatttttt ccacagttgc tctcattgaa gaagataatt cttctttttg actttgaaga
    15301 tataacattt caatataacc ctttaatact gtgatgggag ttcgtaaatc gtgtgatatg
    15361 tttgtaaata tttgcaacat cgctttttca cttctattat aattaatttg cctgctgtaa
    15421 aacttttcaa gaagtttatt tatatctact gctgtttctt ttacaatcga aatatcagat
    15481 ggtactaaaa tataattaat ttcatcatca tttgttatgt cacgaagtct tgaattgata
    15541 tacttataat cactttccat tttctttgat ttatttcgtt cttttaagta gagagcaaat
    15601 agtacaatcg aaattacaac taaaaataac tccattattc ttcctccatt ttgtagccaa
    15661 ttccccaaag agttttaata taattatttt tttctatacc atcagacaat ttatttctta
    15721 acctgttaat atgagtattt agcacatttt catttccata ataaggttct ttccaaatag
    15781 cgttgtataa ttgttccttt gaaaaagcac gtccttgatt agtcgctagt aatttaacaa
    15841 tttcaaattc tgtatgtgtt aagtttaagt caattccatt ctttgaaact acatattttg
    15901 atatatttat aatcaaatct ttatactgaa taaccatatc ttcagagttt cttgaactgc
    15961 tgtacactgt tgcacgtcta atattagctt taattcttgc aagcaattct acgattgaaa
    16021 aaggctttgt aatataatca tctgcaccaa gattaagccc tagcatttta tctgtgtcac
    16081 tatctttcgc agtagtaata ataacaggaa caacactcac acttcgtata tattttaata
    16141 cttcaaaacc actagacttt ggtaacatca agtccagtaa aataatatca aatgaataat
    16201 ttttaaatat ttcaatagct gtatttccat caatggcagt tttaacagag taattctcac
    16261 taatcagaaa atcgtatacc atttctcgaa tattcttatc atcttcaacc aataagatgt
    16321 ccattattat tcaactcctt ttcgtcagta tacttcaaat gatatattta ttaaaaaggg
    16381 ttgtcaataa ataactgctt atgatattgt atctgcaatt tggtgatact cttctaagtt
    16441 aaagttcttt tcaataagct ttattgaaaa aataattaaa atatagtata ctctcttatc
    16501 tttatctgaa atatacataa ttttctttta ttaacatcta caattaacta ttttatatta
    16561 taaaacacct tttaactctt gctaaaaaat attttataca taaaaaagaa accacattct
    16621 agttttatac agaacatagt ttctttaaaa ataatcaaat ttggatttta aataattaaa
    16681 aacaaacttt tatttgtttt taattttcca attttatagt agataattta gttaattagt
    16741 tcttaaatgt ttatcttctt tagtaaattt ttttcttatt aagaaatatg caactattac
    16801 aacaagttct attgctaaaa atacaccata agtagctata gaatctatgc tatctatgcc
    16861 atttctaaag aaaattaaag caattcctgc tgctataata cctacagcat ttttccctat
    16921 agaattttga ccaaaaccat aaaaagaagg gaaatttcct tttatttcaa tgaatgtcaa
    16981 gattaatcca atagctataa atagtactcc tacaacttcc ataatcatgc taggcatttt
    17041 gacccctcct tattaataat taactactac tataaaaata tttattattt ttataattat
    17101 attatatatg cgattacatg tttttggaag tatatttgga agtaatttca tatttattta
    17161 acttaatttt tttgtgataa aatacttcta atttttatat agatattatt ataaattttc
    17221 tagtttatgg agtcattcat tctataattc acaaatatgt attttttaat ctattaaagt
    17281 attcattttc tggcttataa gcatggactt cttatctata agcttggttt ttcatctaat
    17341 aagttattta ttctttaatc tatgagcttg atttttcatc tataagtcat ttatttttta
    17401 atctatgagt ttggtttttc atctataaat aagtcatttt ttaatctata agtttagttc
    17461 ttcatctata aatcatttat tttttaatct ataagtttgg tttttcatct ataaataagt
    17521 catttatttt ttaatctata agtttagttc ttcatctata aatcatttat tttttaatct
    17581 atgagcttga tttttatcta taattaattc attctctaat ctattagctt ggtttttatt
    17641 tataagtcat ttattctcta atctataagc ttacttctta atctataaat cattcatttt
    17701 ttaatctatt agcttggttt ttacctataa atcatttatt ctctaatcta taagctttct
    17761 ttttcatcta taaatcattc attctttaat ccattagct
//
LOCUS       DAYATN010000070        17736 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.070, whole genome shotgun sequence.
ACCESSION   DAYATN010000070 DAYATN010000000
VERSION     DAYATN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.070"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            211..3756
                     /locus_tag="ACGI8A_002276"
     CDS             211..3756
                     /locus_tag="ACGI8A_002276"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0180272.1"
                     /translation="MNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHTY
                     LSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRMT
                     ISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKSG
                     KIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQLF
                     DKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKIV
                     SIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGGV
                     KTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQLE
                     KQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSKR
                     FEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFKD
                     DRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVDI
                     TSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENAT
                     KNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDNN
                     GKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKGA
                     LMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDIS
                     ETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALYS
                     AKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIEV
                     AVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLGN
                     YLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSYN
                     RMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTYE
                     LLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKFE
                     VQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3941..5875
                     /locus_tag="ACGI8A_002277"
     CDS             3941..5875
                     /locus_tag="ACGI8A_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFH0180273.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5981..6976
                     /locus_tag="ACGI8A_002278"
     CDS             5981..6976
                     /locus_tag="ACGI8A_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HFH0180274.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6948..7733
                     /locus_tag="ACGI8A_002279"
     CDS             6948..7733
                     /locus_tag="ACGI8A_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180275.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7721..8419
                     /locus_tag="ACGI8A_002280"
     CDS             7721..8419
                     /locus_tag="ACGI8A_002280"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180276.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8542..9015
                     /gene="ybaK"
                     /locus_tag="ACGI8A_002281"
     CDS             8542..9015
                     /gene="ybaK"
                     /locus_tag="ACGI8A_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFH0180277.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKFYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9212..10051
                     /locus_tag="ACGI8A_002282"
     CDS             9212..10051
                     /locus_tag="ACGI8A_002282"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFH0180278.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKN
                     VVI"
     gene            10064..10612
                     /locus_tag="ACGI8A_002283"
     CDS             10064..10612
                     /locus_tag="ACGI8A_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HFH0180279.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10678..11859
                     /locus_tag="ACGI8A_002284"
     CDS             10678..11859
                     /locus_tag="ACGI8A_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HFH0180280.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12136..12540
                     /locus_tag="ACGI8A_002285"
     CDS             12136..12540
                     /locus_tag="ACGI8A_002285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0180281.1"
                     /translation="MEVQEMMEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSK
                     TLNITAYDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTML
                     YFGAQPGVEAFYEKNGCQRSLQSYIIKKKDSY"
     gene            12690..12953
                     /locus_tag="ACGI8A_002286"
     CDS             12690..12953
                     /locus_tag="ACGI8A_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFH0180282.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13082..13924)
                     /locus_tag="ACGI8A_002287"
     CDS             complement(13082..13924)
                     /locus_tag="ACGI8A_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HFH0180283.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14219..14953
                     /locus_tag="ACGI8A_002288"
     CDS             14219..14953
                     /locus_tag="ACGI8A_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0180284.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14963..16096
                     /gene="nagA"
                     /locus_tag="ACGI8A_002289"
     CDS             14963..16096
                     /gene="nagA"
                     /locus_tag="ACGI8A_002289"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFH0180285.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16195..16944
                     /gene="nagB"
                     /locus_tag="ACGI8A_002290"
     CDS             16195..16944
                     /gene="nagB"
                     /locus_tag="ACGI8A_002290"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFH0180286.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17119..17391
                     /locus_tag="ACGI8A_002291"
     CDS             17119..17391
                     /locus_tag="ACGI8A_002291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180287.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
BASE COUNT         7094 a         1872 c         3127 g         5643 t
ORIGIN      
        1 ataaagttat aatttttaat attaattaag aatgatgaaa tatgaataaa gattttacaa
       61 ataaatctta agctatctta tttaattcta atacaggaag aaggtaaatg tatattgaaa
      121 aaatcataga aatagaagta aacaatttaa ataaatacaa aggtttgcaa aagaagtaat
      181 ataattatat acaatgagga tgtgagtatg atgaacagac taaagccaag aattttaggt
      241 attagtatct tgtttattat tataattatt ttaacatgtg gagctgtttt caagtttaaa
      301 tttaaattag ataataaatt aaaagaaacc acacatacat atttgtctga gtttacagaa
      361 cacaatataa ttaatctcaa gacaagactt agtggtcaat ttgacatgct tgaatctatt
      421 gctaacttca taggaaactt gaataatata aatgatgaaa tggtgattga tttaatggat
      481 tctgatgtaa agcgaagttc tttaattaga atgactatct ctacattaga tggggaatgt
      541 tattctaatg atggaattac ccataatgtt caagatagag attattttaa aaaagctaaa
      601 aatggagaaa agaatatatc agagccttta aaatctattg ttaataataa agaaataata
      661 gttttatcta ccccagttta taaaagtggg aaaatagttg gtgttttaag tggaatttat
      721 gattcaaaaa agttaaatga gttattagga ttatctgcat ttgatgaaaa agcagatgtt
      781 tttgttgcaa agaaaaatgg agatatagtt gctcaaaaaa caaataaaaa tagaaagacg
      841 ctatcatgta tatttcagct ttttgacaaa gataattgtg atgaagatga ttgtctaaaa
      901 actataaaag aaaatatgaa aaatgataag tctggatatt ttaattataa cttagataat
      961 gaaaaattta tgttaaacta tcagccactt ggagttaatg attggtatgt tttttctgtt
     1021 gtgccaaata aaatagtatc tattcagtca gagaatatat cttatgatgt atatattttt
     1081 gcattagaaa tactactagt tatattgatt tttataatat atatagcatg tattataaat
     1141 aataacacca aaattattat aaatgaaaaa gaaaaattta aagtactcac tgataatata
     1201 ttaggagggg ttaaaacctg tttaaaagat gaatatatga ctatgacata tgtaagtcag
     1261 gggtttttag agcttacagg ttactcttgt gaagatttaa ataaaatatt taaaaataga
     1321 tttaaagaca tgatttataa agatgattta gacaagttta attgtattat tctaaatcaa
     1381 ttagaaaaac aagataaaat tgaagttgaa tatagaattg taagaaaaga tggaagtata
     1441 atttggattt tggagagaag taagtctata aatgataata aaggaagtga gtgtattcag
     1501 ggagttttaa ctgatataac agaacttaag aaaatacaag aagaattaga ttcaaaaaga
     1561 tttgaaatac aggctatcaa tgatagtatt gttggtggaa tagtgataac tgagataaat
     1621 gatgattttg atagtatata tttaaatgaa ggttatttaa atatgattgg atatacacgc
     1681 aaacaactgg aagatgagct aaataataaa ttaatcaaac ttatttttaa agatgataga
     1741 acagaattaa ttaatagtat aaacaaacaa ctcaaattaa gtgatacatt tgcatctgaa
     1801 caaagagttg aaaaaaggga tggcactatt atatggattt tattaaaagg aagaaagatg
     1861 atttatgata atggaaaatc tgtactttgt tgtgttattg tagatattac ttctgctaaa
     1921 ttattagagc aagaacttaa aaagagtgaa gaacgtcatg aaatcattct aaagcagacg
     1981 caaaatatta taatagaatg ggatatagaa aaagataaaa tgaatatatc acctaattgg
     2041 tataataagt ttaaatatga ccctataact gaaaacgcaa caaaaaatat aattaaatca
     2101 gtagatatat atcatgatga tatggataag tttatttcat taattaaact tacttcacaa
     2161 ggtagtgagt atgaagagtg tgaaattaga cttagaacta gagatactat ttacgcttgg
     2221 tttagtataa aggcaacaac tttatttgat aataatggta aaccttataa agtagtagga
     2281 agtttgactg atattgatga atcaaaacga gaagctgaaa agttgaaaga aagggcagaa
     2341 aaagaccctc ttacagatct gtataataaa aaagcatcac aatcacttat agaggagtac
     2401 ataacattgg ataaaaaaag aaaaggtgct ttaatgatta ttgatattga tgactttaaa
     2461 ggaataaatg ataatttagg acatttatat ggggatgctg tattaagtga aattgcaaat
     2521 gacctaataa atctatttag agcaaccgat atagtaggca gaattggtgg agatgagttt
     2581 attgtattta taaaagatat ttctgaaaca agagatatta taaataaagc agaagaattg
     2641 gtaaaaacat ttgaaaggtc ttttttaggt aaaggacgaa attataaaat atcattaagt
     2701 gtaggaattg ctaagtttcc acaagatggt atggactata tgacactatt tagaaatgcg
     2761 gatatagctc tttactcagc caaagggaag ggcaagaatt gttatacatt gtatgataaa
     2821 aaatttgata aagtccaata tataactaat acaagaaatg caggagatag tgataatgta
     2881 gagatgtctt ttaaagaaca tatcacaaat tatatatttg atatactata tgaatcaagt
     2941 gatatagaag tggcagtaaa tttaattcta agtattgtag gtaaacactt taatgtgagt
     3001 cgcatgtata tatttgaaaa ttcagatgat aatcgagtat gtaaaaatac atttgaatgg
     3061 tgtaacgaag gtgtaacctc ggaaattaat aatcttaaaa atatttgtta caaggattta
     3121 ggcaactatc ttttaaattt taatgcagat gggttatttt attgtaatga tattgaaaat
     3181 ttaggtagtg atgtgaaaaa agtacttaag ccacaaggca tacgttcaat attacaatgt
     3241 aaaattttag agggtaatga atttagaggg ttcataggat ttgatgaatg ttcatataat
     3301 cgtatgtgga ctaatgatga agtgaacact ttgacattta tatctaaaac gctcagtatg
     3361 tttttattaa aaatgcgtat tcagagtaag ctagaagagt ctttaacaat tcaaaaatct
     3421 gtgatggata atatggatat atgggcatat gttgttgaca ggaataccta tgaattacta
     3481 tttatcaata aaaaaatgct tgagtcagca ccaaacactc atgtaggtga tttatgttat
     3541 aaagcgatat ggaataatca agataaacct tgtaaaaaat gccctatgat atatttaaaa
     3601 gatggtgatg atacttataa atcattcatg cataataaaa agtttgaggt acaggcaaat
     3661 ataactacaa ctactttgaa atggtttgat ggtaaagatg catgtttaat atttggaagt
     3721 gaaataacta gtattgaagc tgaagaaaat acttaaaata ctgtgttata tatatactta
     3781 aaatcgcaat ttgaatttat tagacttaaa atgtctatat aaatcattta agttgtggtt
     3841 ttaagtattt tttatgatat aattaagcgt taagaacaaa attaaaagat acttttgtat
     3901 aaaaaaatat ttgttaaata tatattgagc aggtgataat atggaaaata agtttttacc
     3961 aataagcaaa caagatatga tagatagagg atgggaagaa ttagattttg ttctagttac
     4021 aggagatgct tatgtggacc atcatagttt tggaacagca ataatatcta gagttttaga
     4081 aagtgctggg tataaagttg gaataatagc tcaaccagac tggagaacta cagatgattt
     4141 tatgaagcta ggaaaaccta gacttgcatt tctagttaat gcaggaaata tggattcaat
     4201 ggtcaatcat tattctgtaa gtaaaaagca tagagaaaag gatatgtatt ctccaggtgg
     4261 gaaaatgggt tatagaccag atagagctac tatagtatat tgcaataaaa taagagaagc
     4321 atacagtgat gttgcaatag taataggtgg aattgaagcg agcttaagaa gatttgcaca
     4381 ctatgattat tggagtgata aggttagaaa gtctatgtta atagatagtg gagcagattt
     4441 attagtttat ggtatgagtg aaaagcagat tgtagaagta gccaatgcac ttaatgatgg
     4501 atatgaccca aaatacatta gacatattga tgggacatgc tatatttcag atacattgga
     4561 agaaatatat gataaatata tattaattcc atcatataaa gaaatttgtg aagataaaat
     4621 gaagtatgta gaagctttta aaattcaata tgatgagcaa gacccattta gaggtaatat
     4681 aattgttcaa cctcatggaa gtaagtatct ggttcaaaat aagccagaaa aaccacttag
     4741 tagagaagaa ttagatgaag tatatggatt accatatcag aagacatacc atcctgtata
     4801 tgaaaaattt ggaggaatac cagctataga agaagtaaaa tttagtatag taagttcaag
     4861 aggctgtttt ggtagttgtt cattttgtgc aataacattt catcaaggta gagcagtgca
     4921 aagtagaagt gaaaaatcaa ttattgatga agcaatagga ataacaaatt tagatgattt
     4981 taaagggtat atacatgatg ttggaggtcc aacagctaat tttagaagac cagcatgtaa
     5041 aaaacaaatt actaaaggtg cttgtaaaaa tagacaatgc ttatctccat caccatgtaa
     5101 aaatttagat gcagaccaca gtgaatactt acatttactt agagctgtaa gaaaattacc
     5161 taaaataaaa aaggtgtttg tgcgttctgg aattagatac gattatgtaa tggcagataa
     5221 gaacaataaa tttcttagag agttgataga gcatcatgta agtggtcagt taaaggtagc
     5281 cccagaacat atatcagaag aagttttaaa gtatatgcaa aaaccagcag gaaaaactta
     5341 tgataaattt agacagaagt tttttgctat aaatgaagaa ttaggtaaaa agcaatattt
     5401 gataccctat ttaatgtcta gtcaccctgg ttctacctta aatagcgcta tagagttagc
     5461 agagtattta agagatactc actaccaacc agagcaggtt caggattttt atccaactcc
     5521 agggactcta tctacaacca tgttttatac aggtattgac ccgcttacaa tgaagccagt
     5581 ttatgttcct aaatcaaaac gtgacaaggc aatgcaaagg gctttattac aatatagagc
     5641 acctagaaat tatgatttgg tatactctgc gttagttgaa gcaggaagag aagacttaat
     5701 tggttttgga catagatgtc ttataaagcc aaaaaatgaa aagccttatt ttaatagaaa
     5761 taattctaaa aaaaatgtat ctaaaggaac aaataaaaac aaaaaaacta atacaaataa
     5821 cagaagtaat aaaaatcaac aaaaaagttc aactaaaaaa aagaaaaaac attaaagtat
     5881 aaataaaaat acttaaaaaa gaaggattat tcgataaatt aacagaaatt agtataaata
     5941 aatataatta ttttaatgac gttacctttg ggaggaagag atgaaaaagt atatttctat
     6001 aatattatta gtggtgttga ctatggttgt tttggtagga tgttcaccag gaaaagataa
     6061 ccctaaaaat aaggagctat cagttgtcaa acaagtaaaa gtagctgttc cagatggact
     6121 accagcagtg gcaatagcta aacttgcaaa tgaaaatcca gagattaaag aaggatatga
     6181 aacagtgtat agcattgaaa agacacctga agcgatttcc actagagtga tgaaaaaaga
     6241 tgcagatata gctatagttc cttcaaacat ggctgcaata gcgtataata aaacttcatc
     6301 atataatatt gttggaactg taggaatggg ttcactttat ttggtgtcaa cagaaaatat
     6361 aaaagattac tctgatttag ttggtaaaga agttggatgt actggaaaag gacttactcc
     6421 agacataact ataaaaagtc ttttgttaca aaaggatata aattattcag atataaaatt
     6481 taattatgta aattcagcaa gtgaactggt accattattg gctactggta aagttaaaac
     6541 aggtattgtt ccagaacctg cacttagtgc tttgatgtct aaaaatccag acataaaaat
     6601 tataaaaagt ttaaatgact catggaagga agtaagtggt tcaaaagatg gttatcctca
     6661 atctacttta attgtaaagt ctgaattttt aagagataat aaagattttg ttgattcttt
     6721 tgtaggtcaa ttatcaaata gtatagattg ggcaaacaaa aatcctgaag aattaggtgc
     6781 atattctgaa aaaataaaaa tatctactga atcaaagata ataggaaaat ctcttgaaag
     6841 agctaatttg aaatatattc cagttaagga catgattaaa gattataaaa attactatga
     6901 gaaattagct aattttgatg ataagacatt aggaggcaaa gtaccagatg aagcaatata
     6961 ctttgttgag aaataaaaaa aataaagaac aatttataga taaaatggag gtaacacttt
     7021 cttgtgttat cctcttattt ttatggcaaa ttattgcttt aaaaattgat aatgatattt
     7081 ttttaccaac agtaggtcaa gtgtttaata gtataaaaga gattatgcta aattctagtt
     7141 tctatataga tatactttac tctatgggga gatgtttttt tagtttttta ctggctatga
     7201 tttttgctat tatactttca atagtgtctt atttaaatag attttttaga aacttattaa
     7261 agccaatcaa tgctttgaca cgttcaatac caactatgat attggtagta cttgcactta
     7321 tttggtttga aaaggatagt acccctttta tagttggatt tactatagtt tttcctatat
     7381 tgtatgataa cgttttgggc gctatattga atatagacaa gaacttatta gaaatggcta
     7441 atgtgtataa aataaggttt atagataagg tgttaaaaat ttatttacca gccataaaat
     7501 ttcaaattat atctatacta gtatcaacat tttctttaag tttgaaagtt gctatagcag
     7561 gagaggtgta tagtcaacct acatatggta ttggaactat gatacagact gaaaaaatta
     7621 actttaacac atctggaata tttgcatgga taattattgt tcttttaata tctgctattt
     7681 tacaaatagc tcaaaagttt ttagcaagga gagctttttt atggaaaaga taaagttgag
     7741 tattgaaaat ataaataaaa gatatgatag tcgtattata tttagagatt ttaatattga
     7801 tttttatgta aatgaggtaa attgtatcct tggaaaatct ggttgtggaa aaactacact
     7861 cttgaacata ataagtggga ttattaaaaa tgacaccaac aatctaaata tcaaagaaaa
     7921 tttaaataga gtaggaaata agcttgaagc tagttacata tttcaagatg atagacttat
     7981 agactggtta acagtagaag aaaatataaa gatagttgta aataaatact ataataaaac
     8041 tcaattaaat aaaatttgtg atgaatactt ggaattggtt ggaattagtg attataaaaa
     8101 attctatcct caaatgctta gtggaggtat tagacaaaga gtaaatattg ctagagcttt
     8161 tatatatcca tcaaaaaaca ttataatgga tgaacctttt aaatctattg atgcaaagaa
     8221 tacacagctt ataatggata attttagaaa tatattaaga aaagaaaaga gaactgtgtt
     8281 atttgtaact cataatattg aggaagcatt gtttttagca gataggatat ttattttagg
     8341 agactctcca attagaataa aaaaaatatt aaaaaattct aaggaattgg aaaaaaatga
     8401 agttttgaag ttgatataga gttataataa tactgtcgtt ttaaaatata tttggttaaa
     8461 atctataaga ctatattaaa atagtacaga atattcaaaa ctgtgtataa tgttcgtatt
     8521 ttataaaaag gaagtgaatg tatggttaag actaatgcta tgagaatatt agattcaaat
     8581 aagattgatt ataaagtaat gtcttatgaa gtgaaaagtg aacatgtgga tggtgttgag
     8641 gtggcacatg atataggaag agatgtaaat gaagtgtata agacattagt aactcaagga
     8701 gttagtaaga atatatatgt atatgtaata ccagtacatg aaaacttaga cttaaaaaag
     8761 gctgcaaaag tagctaaaga gaaaagtgta gagatgattc atgtaaaaga tataaataag
     8821 cttacaggtt atattagagg cggatgttct cctataggta tgaaaaagtt ttataaaact
     8881 tttgtaaatg aaagtgcaaa gaatttagat acaattattg tgagtgcagg aaaaatagga
     8941 tatcaaatag aactatctcc atttgattta caaagactta taaaagtaga atttgtagat
     9001 gtaataaaaa aataagaatt acatataaaa tatagagctt atattctcaa taataggtgt
     9061 tgtaatatta ataaaatgaa taagaatttt aaaataaata ttcagttact tatgtgtaat
     9121 tggtataaaa atattgagaa attttaaaaa caatggtaaa gtgtaagtat aatcacgaat
     9181 attttttata aagtaatgga gggatgttcg cttgagaaaa ataaaatctg aacaaatcgt
     9241 cgagcaggta aaaaaacttt gtatagaagc gagcctatat ttaggagaag atgttttaag
     9301 ttgtataaaa gaaaaagcga aaagcgaaaa gagtgaagtt ggaaaaaata tattgaatat
     9361 attagttgaa aatgctgaaa tagctaaaga aaaaaatata ccaatatgcc aagatactgg
     9421 tatggctgta ttttttgttg aaataggaca agaagtttta attgaaggag acacacttac
     9481 tgatgctata aatgagggag taagacaagg gtatgaagaa ggctacttaa gaaaatctgt
     9541 tgtaagtcca attaacagag taaatacaaa agataataca cctgctgtta ttcattatga
     9601 tatggtaaaa ggtgataaaa ttaaaataga atttgcagct aagggatttg gaagcgaaaa
     9661 tatgagtaag atgaaaatgt taaagccatc agatggattg gaaggaatta aaaaattcat
     9721 aatagataca gtttcagaag ctggacctaa cccatgccct cctatggtaa ttggagttgg
     9781 tataggtggg actgtggaca aatgtgctca aatagctaaa aaggcacttt ttagagaact
     9841 aggtgaattt aataaggacg aaaatatagc aaaacttgaa agtgaactat taacagctat
     9901 taataaactt ggtataggac ctcaaggctt aggtggtact acaacagctt tgggattaaa
     9961 catagagact tttcctacac acatagcagg tctacctgtt gttgtaaata taaactgcca
    10021 tgcatcaaga cataagaatg tagtaattta ggaggaaaat aaaatgataa aaataactac
    10081 acctgtaaat gaaattgata tagcaaaatt aaattgtgga gatacaatta gtttgagtgg
    10141 tatactttat acagcaagag atgctgctca taaaagattg atagattgta taaataaagg
    10201 tgaagaatta ccatttgacg tatatggtca aggaatatat tatgtaggac ctactcctac
    10261 aaagccagga gaagttatag gtgctgcagg tccaacgacg agttatagaa tggatgattt
    10321 gactatacct cttttagaaa gaggtcttag attgatgata ggcaagggaa aaagaagtga
    10381 tgaagttata gaaggtatgc aaaaatacgg agcagtttat cttgctgcaa taggaggagc
    10441 tggagcgtat atttcaaatt caataaaatc atgtgaaatt attgcatatg aagatttagg
    10501 agctgaagct ataagaaaga ttgtagtaga agaccttaag cttacagtag caatagatag
    10561 ctatggaaat aatatatatg agcaaggtcg agcaatttat gaatgtaaat aatgtttaga
    10621 aataaatatg aatatcaaaa ttaaaaatag ttgtgtatat tttaaggagg aaaattgatg
    10681 tctaaggatt atgcaaaatt ggcattagaa atgcatgaaa tcaacaaagg gaaagtaagt
    10741 gtagaaagca aggtagaaat taaaactaag gatgatttat caacagcata tacaccaggg
    10801 gttgctgaac cttgtttaaa aatacatgaa aatcaagatg atgtttatag atacacttct
    10861 aaagggaatc tagtagcagt agttagtgat ggaagtgctg tacttggatt aggtaacatt
    10921 ggtgctgaag catctatacc tgttatggaa ggtaaagcta tattatttaa acaatttgca
    10981 gatgtagacg cttttccaat atgtttaaag actaatgatg tagatgagat agttaaatct
    11041 gtagagttga tggagcctgt atttggaggt ataaatttgg aagacatatc ttctccaaga
    11101 tgttttgaga tagaagagag attaaaaaaa tctttaagta tacctgtttt tcatgatgac
    11161 caacatggga cagcaataat agttgcagcc gccataataa actcaataaa gttaattgaa
    11221 aataaaaaaa tagaagattt agaaatagtt ataaacggag caggagctgc tggtatagcc
    11281 atagcaaaga tactacttaa tatgaatgtt aaaaatataa tcttatgtga tagaagtgga
    11341 gcattggaag ctagtataga aaatctaaac tatgttcaaa aagaaatgtt aaaagtaaca
    11401 aatataagaa atgaaaaagg acctttaaaa gatattataa aaggaaaaga tgtatttatt
    11461 ggtgtttcag gtccaggagc tgttacaaag gaaatggttg aaagtatgtc tgagaagcca
    11521 ataatattgg caatggcaaa tcctactcca gagataatgc cagaagaagc aaaattaggt
    11581 ggagcattta taatgggaac aggtcgttca gacttcccta atcaagtcaa taatgtactg
    11641 gcttttcctg gaatatttag aggtgcactt gatgttaagg ctaaagagat aaatgaagaa
    11701 atgaagatag ctgctgcata tgcaattgct aatacaataa gtgatgaaga aatatgccca
    11761 gagtatatat taccagatgt atttaataag aatgtagtaa aaaatgttgc taaggcagtt
    11821 aaagaagcag cgataaaaac taaagtaaat agaatataaa tttttatatg ttcatatatc
    11881 cttatattat cgggtatatg ggcattttta ttataggttt atactcatat atccttactt
    11941 ttatatacat atctttatga ttaaaggtgt tagaaaatgt agtaaatttt atactatcag
    12001 actctatctt tattacttta gtagatacat ggttgtaaag gtttatagaa aaaaatatag
    12061 gaaggcagat ttttattgtt gcgtatatat gagaatgata taatatgaat attataaatt
    12121 aataaattta aatttatgga ggtacaagag atgatggaat ataaagttaa tgaccaagaa
    12181 cttaatgctt caatgtttat cccattcgtc aacaggatat ggccaggaga ttatgatatg
    12241 gataaaacac agactgcatt atcaaaaact ttgaatataa ctgcttatga tgataaagtg
    12301 cttgtaggat gtctgcgagt tctttcagat ggttattatt ttggaacaat tacagaattg
    12361 cttgttcttc cagagtatca gaaacaaggc attggtagta agcttttcca gcttgctaaa
    12421 gagaacaccc caactatgct atattttggt gcacagccag gagtagaagc gttctatgaa
    12481 aaaaatgggt gccaaagaag tctgcaatcc tacataataa aaaagaaaga tagttattaa
    12541 agttccagct ttcaataatc ttaaaagtac aatattttag tggaatcatt tcatttttta
    12601 ttactaatat gacataaaca tgtcatatta gtattgatat acttttaata tggttaagag
    12661 ccaaagaaaa atataaggag tgatttaaaa tggaagaaac taaattttgc caatgctgtg
    12721 caatgccaat gggacaaact gatgaattat atgggacaaa taaagatgga agtaaaagta
    12781 ctgattattg tagttattgt tttaaagatg gtgagtttac agctgatata agtatggaag
    12841 agatgataga agtttgtatc cctcatatgc ttcaaagtaa taaggatatg actgaagatg
    12901 aagcaagaac aatgatgaac aatttctttc caacattaaa acgctggaaa taattttatg
    12961 caaaattagt tatatgcaaa acatgaaata tggtgttgag aataaggggt ggtgtgcttt
    13021 atagcatacc acctgttttg ttacttggag ttcagttatt ttcctaaata ttttaaatat
    13081 actattttag ataagttccc tcttcatctt gataaattaa tccttttttc ataagcactc
    13141 caagagctcg tttaaaattt ttcttacttg aattaaataa tatagaaata tctttagggt
    13201 cagatttatc attaaatctc atatatccat cagaaccttc taaataactt aaaatcttat
    13261 tttctaaagt atcaagttca tcctttctat ttcctctagg agaaagacca agttttccat
    13321 cttcatatat ttttatgaca tgtaaatttt caagaacatc accagttttt agttcagtaa
    13381 agtactcatt atgaagtatt acaccagcat atttattgtc tacacatatc attgcagaat
    13441 tattagtttg gaatccatat acaactccag ttacaatatc tcctacattg taagtatggt
    13501 ctgttaataa gtatgaatct atgtctgtag tagcagcaat tcttttgctt ttgtctaaat
    13561 atatataaaa taaatatttt tcgtctctaa atagtggata agtcttagct ttaaaaggaa
    13621 ctaatatgtc tttaggaagt cccatatcta taaaagtacc aatgtctgta ttatcaacta
    13681 cttttaaatg tgctacatcc ccaaccttag ctaaaggcgg tattaaagtt gcagcagttc
    13741 tgtcatcaga atctttaaat atgaaagcat ttacttcatc acctatgttt atttcatttt
    13801 ttccgattaa tctattatga agaagtatat cgtcttttgt gttattagtt tgtccatcca
    13861 aaaaatatcc aaattcagtt tttcttttta ctgtaagttt attaaaatca cctatgttta
    13921 tcaaattaaa atcatcctct cattttatat ttataattta gccataatat acttatgtag
    13981 tagtttgtca cagtatccat attagtatta gtgaaattta caataaataa tatagttatt
    14041 tatgtatttt tcaattctct agcttcttat atagtataaa ctccaatttc ttttttgtct
    14101 ttaggtttta ttaaaatttt tacaaaataa gttaaaaagt attgaaataa aatttctttt
    14161 aaagtataat tggtatatac caaaattggt atataccata tcgaaaagtg gtgactatat
    14221 gaaaatagta aataaatcta gtaatatacc attgcatact caattaagtt caataataag
    14281 agagatgata gaaactggtg agcttaaaga gggcgatgca ataatgccag aaagagaact
    14341 ttgcaacata caaaatgtaa gtcgtatgac agtaaataaa accattgttg gattagttac
    14401 agaaggactt ctttataggg tacaaggtaa agggacattc gttgcaaagc aaaaaaagaa
    14461 gtatcagttt tcaaatgtaa aaggttttac tgatgttatg aaagaaaagg gagtaaacat
    14521 aaaaacggat atattatcct ttgagatgga actaccagat gatttagtta aaagaaagct
    14581 tggaataagt gataatacta caaatatata taagattgtc agacttagat atactgatgg
    14641 agagccattt ggattagaaa tagtatatct atcagaagaa atgtgtaagg gtctaacaaa
    14701 agggatattg gataattctt cattatatag ggttttaaat gaaaaatatg gctataagat
    14761 acaaaaagct gaacaggtaa tggagccagt aatactttca gatgaagaaa gtaagcttct
    14821 tgaaacagat gaaggtgctt tagctcttaa acttcataga aattcctata atagagaagg
    14881 tagccctata gaatatacca tatcaatatt tagaacagat aaatatcaat atgaaatagt
    14941 attgagtgag taggtgaata atatgaaatg tttaattaat ggaaagataa tattaaaaaa
    15001 tcaaatctta gaaaataaag tattagtatt tgatgaaaaa ataattgata tagctgatag
    15061 tgtaccaaaa gattgtgaag ttatagatgc tgatggaaaa tatatatctc cagggcttat
    15121 tgatattcat atacatggaa acatgggaaa agatacaatg gattcgactg atgaatctat
    15181 tgaaacaatt tcaaagtcaa ttatgagaca tggagtaaca tcttttcttc caactacaat
    15241 gactatggat aaagaacatg tttatgatgc acttgaagtt attaaaaaag cccaaaatag
    15301 aaagcttgaa ggggcacaag tattaggagc acatttagaa ggacctttta taaatgagaa
    15361 ttataaggga gcacaaaatg aaaaatttat aataaattca aaatacgaat ttataaaaga
    15421 atataaagat gttataaaag ttataactta tgctccagaa aaagatattg attttgattt
    15481 tactagagaa ataaaaagat gtacagatat agttttatct attggtcata gtaatgcaaa
    15541 ttatgaccaa gctaaagaag ctataaattt aggagtaact aatgtaactc acatgttcaa
    15601 tgcaatgact ggtttaaacc atagagaccc aggcgttgtt ggtgcagcac ttacaactaa
    15661 tgtgtacagt gagttaatag cagatacaat ccatataaat aaagatttat ttcaatttat
    15721 actaaataat aaaggaaaag aaagactgat tttaataaca gattctatag aagctggtgg
    15781 tcttgaagat ggcaattatt ctcttggagg tcagaaagtc atagttaagg gtaatgaagc
    15841 tagacttgaa aatggagcat tagctggaag tgtgttgtct ttaaataaaa tggtatttaa
    15901 cttcttagat aatacaaacc ttaaagtgaa tgaagcaatt aatttagctt ctttaaatcc
    15961 agcaacatct ttgggaataa atgacaaaaa aggaagctta gaaataggta aggatgcaga
    16021 tatagctgta tttgatgaga atttagattg taaaatgaca ctttgcttgg gggacgtagt
    16081 atataaaaat atatagtata caaaacaaac cttaaagaca gtaaaaacaa tataaaaaat
    16141 aataaattaa acttacatta gtaaaagtag tagttaaaat ttaggaggaa taagatgaga
    16201 gtattagtgt gtaaagatta tgatggaatg agtaaaaaag ccgctgaaat gatagcagct
    16261 caaatagttt taaaaccaaa ttcaatactg ggactagcta caggaagtac accagtaggt
    16321 atgtatagag atttagtaaa aaaatacaat gataatatag tagatttttc agatgtgatg
    16381 agctttaatt tagatgaata ttataagtta ccaatatcta atgaccaaag ctatgattat
    16441 tttatgaaag aaaatctatt taatcatgta aacataaagc cagaaaatac tcatctacca
    16501 aatggaatgg ctgatgatat tgaaaaagag tgtatgaact atgaagcttc tatagatgca
    16561 gctggtggta tagatgtaca agtacttgga ataggtcgta atgctcatat aggttttaat
    16621 gaacctgata ctaaatttgc aaagagaact catgtagttg aacttacaga gtctacaata
    16681 gaagctaatg caagattctt taaatcaaga gaagatgttc ctaaaaaagc tgtaagtatg
    16741 ggaataggtt caatattgaa gtctaagaaa atattgcttt tagcttcagg agaagaaaaa
    16801 gctgatgcag tatataatac tgtttatgga gatataactc cagaagttcc tggttctata
    16861 cttcaacttc ataaagacac tatagttata gtagatgaag cagcagcaag taaattgaat
    16921 ccaaaagatt ataaattagt ataagaatag aatgtagttt aagtaaaaaa tagctatcat
    16981 tgagtaatca aatttattta atgatagcta tttttattta aattttttac ttctttttat
    17041 ttaaattagt tattattgtt taaattactt gcttattact ttttacgaaa actcgttatc
    17101 aagaagtatc caaataagat gttaaaaatt attatgttta gtgggattct atcaagcata
    17161 ataaaattac ctataattgg gatattacca actggaaaca taaagaagaa tacaggcatc
    17221 agtgatagta aaaaagatgg aaaacaaaga agaagtaatc ttggaatatc aatataccaa
    17281 ctaccatcct ttttgtattc tctaatcaat aattcagttc ctagtaataa accaatacca
    17341 ccagaggaaa ttaaattaat aaaaatgtat cttttaaagt cttgcatata accagtgtaa
    17401 aattgagtat agaaatcata atttaattta acagctaaaa aatacataag caatagaatg
    17461 attaaaaata aaagatatct aagagctttt ttcattttaa attctccctt cagaaatact
    17521 ttattcagtt ttattttgtt ggaatctgat tttatcattt agtttaagaa atccataata
    17581 ttagtgaaga atgaaataag tattatatat gaataaatat ttttagaatt aaggtgaatt
    17641 gtagtgctta atataactat aatattaatt gtttgaatat tcaataaaaa gtttgaaaaa
    17701 tatcaatttt gatataaact gaataaatac agttga
//
LOCUS       DAYATN010000071        17653 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.071, whole genome shotgun sequence.
ACCESSION   DAYATN010000071 DAYATN010000000
VERSION     DAYATN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.071"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            52..606
                     /gene="tcdR"
                     /locus_tag="ACGI8A_002292"
     CDS             52..606
                     /gene="tcdR"
                     /locus_tag="ACGI8A_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HFH0180288.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            924..8024
                     /gene="tcdB"
                     /locus_tag="ACGI8A_002293"
                     /gene_synonym="toxB"
     CDS             924..8024
                     /gene="tcdB"
                     /locus_tag="ACGI8A_002293"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Toxigenic strains produce this toxin (TcdB) or
                     TcdA. TcdB-producing strains are tumorigenic in mice;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HFH0180289.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8146..8646
                     /gene="tcdE"
                     /locus_tag="ACGI8A_002294"
     CDS             8146..8646
                     /gene="tcdE"
                     /locus_tag="ACGI8A_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HFH0180290.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8725..8886
                     /locus_tag="ACGI8A_002295"
     CDS             8725..8886
                     /locus_tag="ACGI8A_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180291.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            8951..9157
                     /locus_tag="ACGI8A_002296"
     CDS             8951..9157
                     /locus_tag="ACGI8A_002296"
                     /inference="COORDINATES: protein motif:HMM:NF022413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BhlA/UviB family holin-like peptide"
                     /protein_id="HFH0180292.1"
                     /translation="MKLFKNTKIRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKT
                     LKKYKICLQITIRQSPYLIEESIN"
     gene            9374..17506
                     /gene="tcdA"
                     /locus_tag="ACGI8A_002297"
                     /gene_synonym="toxA"
     CDS             9374..17506
                     /gene="tcdA"
                     /locus_tag="ACGI8A_002297"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HFH0180293.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT         7047 a         1887 c         2791 g         5928 t
ORIGIN      
        1 gtcaaaattt tcaaataaat catcattata aatataagag aggatgattt tatgcaaaag
       61 tctttttatg aattaattgt tttagcaaga aataactcag tagatgattt gcaagaaatt
      121 ttatttatgt ttaagccatt agtaaaaaaa cttagtagag ttttacatta tgaagaggga
      181 gaaacagatt taataatatt ttttattgaa ttaataaaaa atattaaatt aagtagcttt
      241 tcagaaaaaa gcgatgctat tatagtcaaa tatattcata aatcattact gaataagact
      301 tttgagttgt ctagaagata ttctaaaatg aagtttaatt ttgtagaatt tgatgaaaat
      361 atcttaaata tgaaaaataa ttatcaaagt aagtctattt ttgaggaaga tatttgtttt
      421 ttcggatata ttttgaaaga attatctggt attcaaagaa aagttatttt ttataaatat
      481 ttaaaaggat attctgatag agaaatatca gtgaaattaa aaatatctag acaagctgtt
      541 aataaggcta aaaatagagc atttaaaaaa ataaaaaaag actatgaaaa ttattttaac
      601 ttgtaattaa tgagtttaaa gaaatattta caatagaaat caaattttat aattaacttt
      661 attgtaaaat taataactta atctaagaat atcttaattt ttatatttta tatagaacaa
      721 tgtttacata tttatttcag acaacgtctt tattcaatcg aagagcaaat taatcaactg
      781 agtgtcttaa atttaaaatg ttaggaagtg aatgtatatg aaaacctaag tagatattag
      841 tatattttat aaatagaaag gaggatatat aaaagagttt tagcatttag atgtaaaaat
      901 attatagtaa aggagaaaat tttatgagtt tagttaatag aaaacagtta gaaaaaatgg
      961 caaatgtaag atttcgtact caagaagatg aatatgttgc aatattggat gctttagaag
     1021 aatatcataa tatgtcagag aatactgtag tcgaaaaata tttaaaatta aaagatataa
     1081 atagtttaac agatatttat atagatacat ataaaaaatc tggtagaaat aaagccttaa
     1141 aaaaatttaa ggaatatcta gttacagaag tattagagct aaagaataat aatttaactc
     1201 cagttgagaa aaatttacat tttgtttgga ttggaggtca aataaatgac actgctatta
     1261 attatataaa tcaatggaaa gatgtaaata gtgattataa tgttaatgtt ttttatgata
     1321 gtaatgcatt tttgataaac acattgaaaa aaactgtagt agaatcagca ataaatgata
     1381 cacttgaatc atttagagaa aacttaaatg accctagatt tgactataat aaattcttca
     1441 gaaaacgtat ggaaataatt tatgataaac agaaaaattt cataaactac tataaagctc
     1501 aaagagaaga aaatcctgaa cttataattg atgatattgt aaagacatat ctttcaaatg
     1561 agtattcaaa ggagatagat gaacttaata cctatattga agaatcctta aataaaatta
     1621 cacagaatag tggaaatgat gttagaaact ttgaagaatt taaaaatgga gagtcattca
     1681 acttatatga acaagagttg gtagaaaggt ggaatttagc tgctgcttct gacatattaa
     1741 gaatatctgc attaaaagaa attggtggta tgtatttaga tgttgatatg ttaccaggaa
     1801 tacaaccaga cttatttgag tctatagaga aacctagttc agtaacagtg gatttttggg
     1861 aaatgacaaa gttagaagct ataatgaaat acaaagaata tataccagaa tatacctcag
     1921 aacattttga catgttagac gaagaagttc aaagtagttt tgaatctgtt ctagcttcta
     1981 agtcagataa atcagaaata ttctcatcac ttggtgatat ggaggcatca ccactagaag
     2041 ttaaaattgc atttaatagt aagggtatta taaatcaagg gctaatttct gtgaaagact
     2101 catattgtag caatttaata gtaaaacaaa tcgagaatag atataaaata ttgaataata
     2161 gtttaaatcc agctattagc gaggataatg attttaatac tacaacgaat acctttattg
     2221 atagtataat ggctgaagct aatgcagata atggtagatt tatgatggaa ctaggaaagt
     2281 atttaagagt tggtttcttc ccagatgtta aaactactat taacttaagt ggccctgaag
     2341 catatgcggc agcttatcaa gatttattaa tgtttaaaga aggcagtatg aatatccatt
     2401 tgatagaagc tgatttaaga aactttgaaa tctctaaaac taatatttct caatcaactg
     2461 aacaagaaat ggctagctta tggtcatttg acgatgcaag agctaaagct caatttgaag
     2521 aatataaaag gaattatttt gaaggttctc ttggtgaaga tgataatctt gatttttctc
     2581 aaaatatagt agttgacaag gagtatcttt tagaaaaaat atcttcatta gcaagaagtt
     2641 cagagagagg atatatacac tatattgttc agttacaagg agataaaatt agttatgaag
     2701 cagcatgtaa cttatttgca aagactcctt atgatagtgt actatttcag aaaaatatag
     2761 aagattcaga aattgcatat tattataatc ctggagatgg tgaaatacaa gaaatagaca
     2821 agtataaaat tccaagtata atttctgata gacctaagat taaattaaca tttattggtc
     2881 atggtaaaga tgaatttaat actgatatat ttgcaggttt tgatgtagat tcattatcca
     2941 cagaaataga agcagcaata gatttagcta aagaggatat ttctcctaag tcaatagaaa
     3001 taaatttatt aggatgtaat atgtttagct actctatcaa cgtagaggag acttatcctg
     3061 gaaaattatt acttaaagtt aaagataaaa tatcagaatt aatgccatct ataagtcaag
     3121 actctattat agtaagtgca aatcaatatg aagttagaat aaatagtgaa ggaagaagag
     3181 aattattgga tcattctggt gaatggataa ataaagaaga aagtattata aaggatattt
     3241 catcaaaaga atatatatca tttaatccta aagaaaataa aattacagta aaatctaaaa
     3301 atttacctga gctatctaca ttattacaag aaattagaaa taattctaat tcaagtgata
     3361 ttgaactaga agaaaaagta atgttaacag aatgtgagat aaatgttatt tcaaatatag
     3421 atacgcaaat tgttgaggaa aggattgaag aagctaagaa tttaacttct gactctatta
     3481 attatataaa agatgaattt aaactaatag aatctatttc tgatgcacta tgtgacttaa
     3541 aacaacagaa tgaattagaa gattctcatt ttatatcttt tgaggacata tcagagactg
     3601 atgagggatt tagtataaga tttattaata aagaaactgg agaatctata tttgtagaaa
     3661 ctgaaaaaac aatattctct gaatatgcta atcatataac tgaagagatt tctaagataa
     3721 aaggtactat atttgatact gtaaatggta agttagtaaa aaaagtaaat ttagatacta
     3781 cacacgaagt aaatacttta aatgctgcat tttttataca atcattaata gaatataata
     3841 gttctaaaga atctcttagt aatttaagtg tagcaatgaa agtccaagtt tacgctcaat
     3901 tatttagtac tggtttaaat actattacag atgcagccaa agttgttgaa ttagtatcaa
     3961 ctgcattaga tgaaactata gacttacttc ctacattatc tgaaggatta cctataattg
     4021 caactattat agatggtgta agtttaggtg cagcaatcaa agagctaagt gaaacgagtg
     4081 acccattatt aagacaagaa atagaagcta agataggtat aatggcagta aatttaacaa
     4141 cagctacaac tgcaatcatt acttcatctt tgggggtagc tagtggattt agtatacttt
     4201 tagttccttt agcaggaatt tcagcaggta taccaagctt agtaaacaat gaacttgtac
     4261 ttcgagataa ggcaacaaag gttgtagatt attttaaaca tgtttcatta gttgaaactg
     4321 aaggagtatt tactttatta gatgataaaa taatgatgcc acaagatgat ttagtgatat
     4381 cagaaataga ttttaataat aattcaatag ttttaggtaa atgtgaaatc tggagaatgg
     4441 aaggtggttc aggtcatact gtaactgatg atatagatca cttcttttca gcaccatcaa
     4501 taacatatag agagccacac ttatctatat atgacgtatt ggaagtacaa aaagaagaac
     4561 ttgatttgtc aaaagattta atggtattac ctaatgctcc aaatagagta tttgcttggg
     4621 aaacaggatg gacaccaggt ttaagaagct tagaaaatga tggcacaaaa ctgttagacc
     4681 gtataagaga taactatgaa ggtgagtttt attggagata ttttgctttt atagctgatg
     4741 ctttaataac aacattaaaa ccaagatatg aagatactaa tataagaata aatttagata
     4801 gtaatactag aagttttata gttccaataa taactacaga atatataaga gaaaaattat
     4861 catattcttt ctatggttca ggaggaactt atgcattgtc tctttctcaa tataatatgg
     4921 gtataaatat agaattaagt gaaagtgatg tttggattat agatgttgat aatgttgtga
     4981 gagatgtaac tatagaatct gataaaatta aaaaaggtga tttaatagaa ggtattttat
     5041 ctacactaag tattgaagag aataaaatta tcttaaatag ccatgagatt aatttttctg
     5101 gtgaggtaaa tggaagtaat ggatttgttt ctttaacatt ttcaatttta gaaggaataa
     5161 atgcaattat agaagttgat ttattatcta aatcatataa attacttatt tctggcgaat
     5221 taaaaatatt gatgttaaat tcaaatcata ttcaacagaa aatagattat ataggattca
     5281 atagcgaatt acagaaaaat ataccatata gctttgtaga tagtgaagga aaagagaatg
     5341 gttttattaa tggttcaaca aaagaaggtt tatttgtatc tgaattacct gatgtagttc
     5401 ttataagtaa ggtttatatg gatgatagta agccttcatt tggatattat agtaataatt
     5461 tgaaagatgt caaagttata actaaagata atgttaatat attaacaggt tattatctta
     5521 aggatgatat aaaaatctct ctttctttga ctctacaaga tgaaaaaact ataaagttaa
     5581 atagtgtgca tttagatgaa agtggagtag ctgagatttt gaagttcatg aatagaaaag
     5641 gtaatacaaa tacttcagat tctttaatga gctttttaga aagtatgaat ataaaaagta
     5701 ttttcgttaa tttcttacaa tctaatatta agtttatatt agatgctaat tttataataa
     5761 gtggtactac ttctattggc caatttgagt ttatttgtga tgaaaatgat aatatacaac
     5821 catatttcat taagtttaat acactagaaa ctaattatac tttatatgta ggaaatagac
     5881 aaaatatgat agtggaacca aattatgatt tagatgattc tggagatata tcttcaactg
     5941 ttatcaattt ctctcaaaag tatctttatg gaatagacag ttgtgttaat aaagttgtaa
     6001 tttcaccaaa tatttataca gatgaaataa atataacgcc tgtatatgaa acaaataata
     6061 cttatccaga agttattgta ttagatgcaa attatataaa tgaaaaaata aatgttaata
     6121 tcaatgatct atctatacga tatgtatgga gtaatgatgg taatgatttt attcttatgt
     6181 caactagtga agaaaataag gtgtcacaag ttaaaataag attcgttaat gtttttaaag
     6241 ataagacttt ggcaaataag ctatctttta actttagtga taaacaagat gtacctgtaa
     6301 gtgaaataat cttatcattt acaccttcat attatgagga tggattgatt ggctatgatt
     6361 tgggtctagt ttctttatat aatgagaaat tttatattaa taactttgga atgatggtat
     6421 ctggattaat atatattaat gattcattat attattttaa accaccagta aataatttga
     6481 taactggatt tgtgactgta ggcgatgata aatactactt taatccaatt aatggtggag
     6541 ctgcttcaat tggagagaca ataattgatg acaaaaatta ttatttcaac caaagtggag
     6601 tgttacaaac aggtgtattt agtacagaag atggatttaa atattttgcc ccagctaata
     6661 cacttgatga aaacctagaa ggagaagcaa ttgattttac tggaaaatta attattgacg
     6721 aaaatattta ttattttgat gataattata gaggagctgt agaatggaaa gaattagatg
     6781 gtgaaatgca ctattttagc ccagaaacag gtaaagcttt taaaggtcta aatcaaatag
     6841 gtgattataa atactatttc aattctgatg gagttatgca aaaaggattt gttagtataa
     6901 atgataataa acactatttt gatgattctg gtgttatgaa agtaggttac actgaaatag
     6961 atggcaagca tttctacttt gctgaaaacg gagaaatgca aataggagta tttaatacag
     7021 aagatggatt taaatatttt gctcatcata atgaagattt aggaaatgaa gaaggtgaag
     7081 aaatctcata ttctggtata ttaaatttca ataataaaat ttactatttt gatgattcat
     7141 ttacagctgt agttggatgg aaagatttag aggatggttc aaagtattat tttgatgaag
     7201 atacagcaga agcatatata ggtttgtcat taataaatga tggtcaatat tattttaatg
     7261 atgatggaat tatgcaagtt ggatttgtca ctataaatga taaagtcttc tacttctctg
     7321 actctggaat tatagaatct ggagtacaaa acatagatga caattatttc tatatagatg
     7381 ataatggtat agttcaaatt ggtgtatttg atacttcaga tggatataaa tattttgcac
     7441 ctgctaatac tgtaaatgat aatatttacg gacaagcagt tgaatatagt ggtttagtta
     7501 gagttgggga agatgtatat tattttggag aaacatatac aattgagact ggatggatat
     7561 atgatatgga aaatgaaagt gataaatatt atttcaatcc agaaactaaa aaagcatgca
     7621 aaggtattaa tttaattgat gatataaaat attattttga tgagaagggc ataatgagaa
     7681 cgggtcttat atcatttgaa aataataatt attactttaa tgagaatggt gaaatgcaat
     7741 ttggttatat aaatatagaa gataagatgt tctattttgg tgaagatggt gtcatgcaga
     7801 ttggagtatt taatacacca gatggattta aatactttgc acatcaaaat actttggatg
     7861 agaattttga gggagaatca ataaactata ctggttggtt agatttagat gaaaagagat
     7921 attattttac agatgaatat attgcagcaa ctggttcagt tattattgat ggtgaggagt
     7981 attattttga tcctgataca gctcaattag tgattagtga atagataaaa atatgttaaa
     8041 tatatcctct tatacttaaa tatataaaaa taaacaaaat gatacactac ataaagtgtt
     8101 ctatctaata tgaagattta ccaataaaaa ggtggactat gatgaatgca cagtagttca
     8161 cctttttata tttctaatgg taacaaaata tttttttata taaacctagg aggcgttatg
     8221 aatatgacaa tatctttttt atcagagcat atatttataa agttagtaat tttaactata
     8281 tcatttgata cattattagg atgtttaagt gcaataaaaa gtcgtaaatt taattctagt
     8341 tttggaatag atggaggaat cagaaaagta gcaatgatag catgtatatt ttttttatca
     8401 gtagttgaca ttcttacaaa gtttaacttt ttatttatgt taccacaaga ttgtatcaat
     8461 tttttaagac taaaacatct tggaatatct gaatttttct ctattttatt tattttatat
     8521 gaaagtgtaa gtatattaaa aaatatgtgc ttatgtggat taccagtacc taagagatta
     8581 aaggaaaaaa tagcaatttt actagatgca atgacagatg aaatgaatgc taaggatgaa
     8641 aagtaagtaa tggtagatat aataaagata ttaacaaata aaaagtgtta tccaaataag
     8701 aatagctgaa agttatcata attcatgaaa ctaataatga aaacgaggga gcagatgcca
     8761 agagacacac aagtattaaa tacatataat ttcgaagcaa gtgttcatta ctatatagat
     8821 gacaaggtag tatatcaaac attggttcac aaagatggtg catggtcagt tggtaaaatc
     8881 tattaagcta cattagttac agatatcaca aactataata gttaaacata gaaatatgtg
     8941 taaattgtga ttgaaattat tcaaaaacac aaaaatacgt gatgaaggac aaaatgatat
     9001 agaaaataag tatcaaacct taataaatga tttaattgat agtttaaaag ttataggaaa
     9061 aatatataaa gaaataaaaa cattaaaaaa atataagata tgtttacaaa ttactatcag
     9121 acaatctcct tatctaatag aagagtcaat taactaattg agtatcttta aattgaaatg
     9181 ttaggaagtg atttaaatat gaaaacttaa attataaaaa atcaatatta atttattttt
     9241 aaaaaataga aaggagtgta taagatttat tttcaaagtt taaaaacaag aaaatcaatt
     9301 taaatttcag aaggaataaa tgtggttata gaagtggatt tattatcaaa aataataata
     9361 ctaggaggtt tttatgtctt taatatctaa agaagagtta ataaaactcg catatagcat
     9421 tagaccaaga gaaaatgagt ataaaactat actaactaat ttagacgaat ataataagtt
     9481 aactacaaac aataatgaaa ataaatattt acaattaaaa aaactaaatg aatcaattga
     9541 tgtttttatg aataaatata aaacttcaag cagaaataga gcactctcta atctaaaaaa
     9601 agatatatta aaagaagtaa ttcttattaa aaattccaat acaagccctg tagaaaaaaa
     9661 tttacatttt gtatggatag gtggagaagt cagtgatatt gctcttgaat acataaaaca
     9721 atgggctgat attaatgcag aatataatat taaactgtgg tatgatagtg aagcattctt
     9781 agtaaataca ctaaaaaagg ctatagttga atcttctacc actgaagcat tacagctact
     9841 agaggaagag attcaaaatc ctcaatttga taatatgaaa ttttacaaaa aaaggatgga
     9901 atttatatat gatagacaaa aaaggtttat aaattattat aaatctcaaa tcaataaacc
     9961 tacagtacct acaatagatg atattataaa gtctcatcta gtatctgaat ataatagaga
    10021 tgaaactgta ttagaatcat atagaacaaa ttctttgaga aaaataaata gtaatcatgg
    10081 gatagatatc agggctaata gtttgtttac agaacaagag ttattaaata tttatagtca
    10141 ggagttgtta aatcgtggaa atttagctgc agcatctgac atagtaagat tattagccct
    10201 aaaaaatttt ggcggagtat atttagatgt tgatatgctt ccaggtattc actctgattt
    10261 atttaaaaca atatctagac ctagctctat tggactagac cgttgggaaa tgataaaatt
    10321 agaggctatt atgaagtata aaaaatatat aaataattat acatcagaaa actttgataa
    10381 acttgatcaa caattaaaag ataattttaa actcattata gaaagtaaaa gtgaaaaatc
    10441 tgagatattt tctaaattag aaaatttaaa tgtatctgat cttgaaatta aaatagcttt
    10501 cgctttaggc agtgttataa atcaagcctt gatatcaaaa caaggttcat atcttactaa
    10561 cctagtaata gaacaagtaa aaaatagata tcaattttta aaccaacacc ttaacccagc
    10621 catagagtct gataataact tcacagatac tactaaaatt tttcatgatt cattatttaa
    10681 ttcagctacc gcagaaaact ctatgttttt aacaaaaata gcaccatact tacaagtagg
    10741 ttttatgcca gaagctcgct ccacaataag tttaagtggt ccaggagctt atgcgtcagc
    10801 ttactatgat ttcataaatt tacaagaaaa tactatagaa aaaactttaa aagcatcaga
    10861 tttaatagaa tttaaattcc cagaaaataa tctatctcaa ttgacagaac aagaaataaa
    10921 tagtctatgg agctttgatc aagcaagtgc aaaatatcaa tttgagaaat atgtaagaga
    10981 ttatactggt ggatctcttt ctgaagacaa tggggtagac tttaataaaa atactgccct
    11041 cgacaaaaac tatttattaa ataataaaat tccatcaaac aatgtagaag aagctggaag
    11101 taaaaattat gttcattata tcatacagtt acaaggagat gatataagtt atgaagcaac
    11161 atgcaattta ttttctaaaa atcctaaaaa tagtattatt atacaacgaa atatgaatga
    11221 aagtgcaaaa agctactttt taagtgatga tggagaatct attttagaat taaataaata
    11281 taggatacct gaaagattaa aaaataagga aaaagtaaaa gtaaccttta ttggacatgg
    11341 taaagatgaa ttcaacacaa gcgaatttgc tagattaagt gtagattcac tttccaatga
    11401 gataagttca tttttagata ccataaaatt agatatatca cctaaaaatg tagaagtaaa
    11461 cttacttgga tgtaatatgt ttagttatga ttttaatgtt gaagaaactt atcctgggaa
    11521 gttgctatta agtattatgg acaaaattac ttccacttta cctgatgtaa ataaaaattc
    11581 tattactata ggagcaaatc aatatgaagt aagaattaat agtgagggaa gaaaagaact
    11641 tctggctcac tcaggtaaat ggataaataa agaagaagct attatgagcg atttatctag
    11701 taaagaatac attttttttg attctataga taataagcta aaagcaaagt ccaagaatat
    11761 tccaggatta gcatcaatat cagaagatat aaaaacatta ttacttgatg caagtgttag
    11821 tcctgataca aaatttattt taaataatct taagcttaat attgaatctt ctattggtga
    11881 ttacatttat tatgaaaaat tagagcctgt taaaaatata attcacaatt ctatagatga
    11941 tttaatagat gagttcaatc tacttgaaaa tgtatctgat gaattatatg aattaaaaaa
    12001 attaaataat ctagatgaga agtatttaat atcttttgaa gatatctcaa aaaataattc
    12061 aacttactct gtaagattta ttaacaaaag taatggtgag tcagtttatg tagaaacaga
    12121 aaaagaaatt ttttcaaaat atagcgaaca tattacaaaa gaaataagta ctataaagaa
    12181 tagtataatt acagatgtta atggtaattt attggataat atacagttag atcatacttc
    12241 tcaagttaat acattaaacg cagcattctt tattcaatca ttaatagatt atagtagcaa
    12301 taaagatgta ctgaatgatt taagtacctc agttaaggtt caactttatg ctcaactatt
    12361 tagtacaggt ttaaatacta tatatgactc tatccaatta gtaaatttaa tatcaaatgc
    12421 agtaaatgat actataaatg tactacctac aataacagag gggataccta ttgtatctac
    12481 tatattagac ggaataaact taggtgcagc aattaaggaa ttactagacg aacatgaccc
    12541 attactaaaa aaagaattag aagctaaggt gggtgtttta gcaataaata tgtcattatc
    12601 tatagctgca actgtagctt caattgttgg aataggtgct gaagttacta ttttcttatt
    12661 acctatagct ggtatatctg caggaatacc ttcattagtt aataatgaat taatattgca
    12721 tgataaggca acttcagtgg taaactattt taatcatttg tctgaatcta aaaaatatgg
    12781 ccctcttaaa acagaagatg ataaaatttt agttcctatt gatgatttag taatatcaga
    12841 aatagatttt aataataatt cgataaaact aggaacatgt aatatattag caatggaggg
    12901 gggatcagga cacacagtga ctggtaatat agatcacttt ttctcatctc catctataag
    12961 ttctcatatt ccttcattat caatttattc tgcaataggt atagaaacag aaaatctaga
    13021 tttttcaaaa aaaataatga tgttacctaa tgctccttca agagtgtttt ggtgggaaac
    13081 tggagcagtt ccaggtttaa gatcattgga aaatgacgga actagattac ttgattcaat
    13141 aagagattta tacccaggta aattttactg gagattctat gcttttttcg attatgcaat
    13201 aactacatta aaaccagttt atgaagacac taatattaaa attaaactag ataaagatac
    13261 tagaaacttc ataatgccaa ctataactac taacgaaatt agaaacaaat tatcttattc
    13321 atttgatgga gcaggaggaa cttactcttt attattatct tcatatccaa tatcaacgaa
    13381 tataaattta tctaaagatg atttatggat atttaatatt gataatgaag taagagaaat
    13441 atctatagaa aatggtacta ttaaaaaagg aaagttaata aaagatgttt taagtaaaat
    13501 tgatataaat aaaaataaac ttattatagg caatcaaaca atagattttt caggcgatat
    13561 agataataaa gatagatata tattcttgac ttgtgagtta gatgataaaa ttagtttaat
    13621 aatagaaata aatcttgttg caaaatctta tagtttgtta ttgtctgggg ataaaaatta
    13681 tttgatatcc aatttatcta atactattga gaaaatcaat actttaggcc tagatagtaa
    13741 aaatatagcg tacaattaca ctgatgaatc taataataaa tattttggag ctatatctaa
    13801 aacaagtcaa aaaagcataa tacattataa aaaagacagt aaaaatatat tagaatttta
    13861 taatgacagt acattagaat ttaacagtaa agattttatt gctgaagata taaatgtatt
    13921 tatgaaagat gatattaata ctataacagg aaaatactat gttgataata atactgataa
    13981 aagtatagat ttctctattt ctttagttag taaaaatcaa gtaaaagtaa atggattata
    14041 tttaaatgaa tccgtatact catcttacct tgattttgtg aaaaattcag atggacacca
    14101 taatacttct aattttatga atttattttt ggacaatata agtttctgga aattgtttgg
    14161 gtttgaaaat ataaattttg taatcgataa atactttacc cttgttggta aaactaatct
    14221 tggatatgta gaatttattt gtgacaataa taaaaatata gatatatatt ttggtgaatg
    14281 gaaaacatcg tcatctaaaa gcactatatt tagcggaaat ggtagaaatg ttgtagtaga
    14341 gcctatatat aatcctgata cgggtgaaga tatatctact tcactagatt tttcctatga
    14401 acctctctat ggaatagata gatatatcaa taaagtattg atagcacctg atttatatac
    14461 aagtttaata aatattaata ccaattatta ttcaaatgag tactaccctg agattatagt
    14521 tcttaaccca aatacattcc acaaaaaagt aaatataaat ttagatagtt cttcttttga
    14581 gtataaatgg tctacagaag gaagtgactt tattttagtt agatacttag aagaaagtaa
    14641 taaaaaaata ttacaaaaaa taagaatcaa aggtatctta tctaatactc aatcatttaa
    14701 taaaatgagt atagatttta aagatattaa aaaactatca ttaggatata taatgagtaa
    14761 ttttaaatca tttaattctg aaaatgaatt agatagagat catttaggat ttaaaataat
    14821 agataataaa acttattact atgatgaaga tagtaaatta gttaaaggat taatcaatat
    14881 aaataattca ttattctatt ttgatcctat agaatttaac ttagtaactg gatggcaaac
    14941 tatcaatggt aaaaaatatt attttgatat aaatactgga gcagctttaa ctagttataa
    15001 aattattaat ggtaaacact tttattttaa taatgatggt gtgatgcagt tgggagtatt
    15061 taaaggacct gatggatttg aatattttgc acctgccaat actcaaaata ataacataga
    15121 aggtcaggct atagtttatc aaagtaaatt cttaactttg aatggcaaaa aatattattt
    15181 tgataatgac tcaaaagcag tcactggatg gagaattatt aacaatgaga aatattactt
    15241 taatcctaat aatgctattg ctgcagtcgg attgcaagta attgacaata ataagtatta
    15301 tttcaatcct gacactgcta tcatctcaaa aggttggcag actgttaatg gtagtagata
    15361 ctactttgat actgataccg ctattgcctt taatggttat aaaactattg atggtaaaca
    15421 cttttatttt gatagtgatt gtgtagtgaa aataggtgtg tttagtacct ctaatggatt
    15481 tgaatatttt gcacctgcta atacttataa taataacata gaaggtcagg ctatagttta
    15541 tcaaagtaaa ttcttaactt tgaatggtaa aaaatattac tttgataata actcaaaagc
    15601 agttaccgga tggcaaacta ttgatagtaa aaaatattac tttaatacta acactgctga
    15661 agcagctact ggatggcaaa ctattgatgg taaaaaatat tactttaata ctaacactgc
    15721 tgaagcagct actggatggc aaactattga tggtaaaaaa tattacttta atactaacac
    15781 tgctatagct tcaactggtt atacaattat taatggtaaa catttttatt ttaatactga
    15841 tggtattatg cagataggag tgtttaaagg acctaatgga tttgaatatt ttgcacctgc
    15901 taatacggat gctaacaaca tagaaggtca agctatactt taccaaaatg aattcttaac
    15961 tttgaatggt aaaaaatatt actttggtag tgactcaaaa gcagttactg gatggagaat
    16021 tattaacaat aagaaatatt actttaatcc taataatgct attgctgcaa ttcatctatg
    16081 cactataaat aatgacaagt attactttag ttatgatgga attcttcaaa atggatatat
    16141 tactattgaa agaaataatt tctattttga tgctaataat gaatctaaaa tggtaacagg
    16201 agtatttaaa ggacctaatg gatttgagta ttttgcacct gctaatactc acaataataa
    16261 catagaaggt caggctatag tttaccagaa caaattctta actttgaatg gcaaaaaata
    16321 ttattttgat aatgactcaa aagcagttac tggatggcaa accattgatg gtaaaaaata
    16381 ttactttaat cttaacactg ctgaagcagc tactggatgg caaactattg atggtaaaaa
    16441 atattacttt aatcttaaca ctgctgaagc agctactgga tggcaaacta ttgatggtaa
    16501 aaaatattac tttaatacta acactttcat agcctcaact ggttatacaa gtattaatgg
    16561 taaacatttt tattttaata ctgatggtat tatgcagata ggagtgttta aaggacctaa
    16621 tggatttgaa tactttgcac ctgctaatac tcataataat aacatagaag gtcaagctat
    16681 actttaccaa aataaattct taactttgaa tggtaaaaaa tattactttg gtagtgactc
    16741 aaaagcagtt accggattgc gaactattga tggtaaaaaa tattacttta atactaacac
    16801 tgctgttgca gttactggat ggcaaactat taatggtaaa aaatactact ttaatactaa
    16861 cacttctata gcttcaactg gttatacaat tattagtggt aaacattttt attttaatac
    16921 tgatggtatt atgcagatag gagtgtttaa aggacctgat ggatttgaat actttgcacc
    16981 tgctaataca gatgctaaca atatagaagg tcaagctata cgttatcaaa atagattcct
    17041 atatttacat gacaatatat attattttgg taataattca aaagcggcta ctggttgggt
    17101 aactattgat ggtaatagat attacttcga gcctaataca gctatgggtg cgaatggtta
    17161 taaaactatt gataataaaa atttttactt tagaaatggt ttacctcaga taggagtgtt
    17221 taaagggtct aatggatttg aatactttgc acctgctaat acggatgcta acaatataga
    17281 aggtcaagct atacgttatc aaaatagatt cctacattta cttggaaaaa tatattactt
    17341 tggtaataat tcaaaagcag ttactggatg gcaaactatt aatggtaaag tatattactt
    17401 tatgcctgat actgctatgg ctgcagctgg tggacttttc gagattgatg gtgttatata
    17461 tttctttggt gttgatggag taaaagcccc tgggatatat ggctaaaata tatgtttgat
    17521 aaaaaattat tcctgtgcta ctaagaaatt atttttatat aataaatatt gagatttaat
    17581 taaaagtcat gtgttattgt aatacatgac ttttagttaa aatttttcta tcatttaata
    17641 atctattatt ctt
//
LOCUS       DAYATN010000072        17532 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.072, whole genome shotgun sequence.
ACCESSION   DAYATN010000072 DAYATN010000000
VERSION     DAYATN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.072"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(50..670)
                     /locus_tag="ACGI8A_002298"
     CDS             complement(50..670)
                     /locus_tag="ACGI8A_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFH0180294.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(984..3122)
                     /gene="vanT"
                     /locus_tag="ACGI8A_002299"
     CDS             complement(984..3122)
                     /gene="vanT"
                     /locus_tag="ACGI8A_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HFH0180295.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(3140..3946)
                     /locus_tag="ACGI8A_002300"
     CDS             complement(3140..3946)
                     /locus_tag="ACGI8A_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFH0180296.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3943..5043)
                     /gene="vanG"
                     /locus_tag="ACGI8A_002301"
     CDS             complement(3943..5043)
                     /gene="vanG"
                     /locus_tag="ACGI8A_002301"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HFH0180297.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(5304..6386)
                     /gene="vanS"
                     /locus_tag="ACGI8A_002302"
     CDS             complement(5304..6386)
                     /gene="vanS"
                     /locus_tag="ACGI8A_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFH0180298.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            complement(6436..7137)
                     /locus_tag="ACGI8A_002303"
     CDS             complement(6436..7137)
                     /locus_tag="ACGI8A_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HFH0180299.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(7571..10102)
                     /locus_tag="ACGI8A_002304"
     CDS             complement(7571..10102)
                     /locus_tag="ACGI8A_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0180300.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(10210..10827)
                     /locus_tag="ACGI8A_002305"
     CDS             complement(10210..10827)
                     /locus_tag="ACGI8A_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFH0180301.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(11189..12073)
                     /locus_tag="ACGI8A_002306"
     CDS             complement(11189..12073)
                     /locus_tag="ACGI8A_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFH0180302.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(12264..13514)
                     /locus_tag="ACGI8A_002307"
     CDS             complement(12264..13514)
                     /locus_tag="ACGI8A_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0180303.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(13736..14500)
                     /locus_tag="ACGI8A_002308"
     CDS             complement(13736..14500)
                     /locus_tag="ACGI8A_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180304.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(14494..15219)
                     /locus_tag="ACGI8A_002309"
     CDS             complement(14494..15219)
                     /locus_tag="ACGI8A_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180305.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(15221..15589)
                     /locus_tag="ACGI8A_002310"
     CDS             complement(15221..15589)
                     /locus_tag="ACGI8A_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0180306.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(15765..17309)
                     /locus_tag="ACGI8A_002311"
     CDS             complement(15765..17309)
                     /locus_tag="ACGI8A_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFH0180307.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         6005 a         3085 c         1761 g         6681 t
ORIGIN      
        1 aaataattta gtactaaatc attttaagat acgttttatt tcgtacaagc taaatcattt
       61 tatcatcttt atcattgtga tgttttttat gatggaaatc ataattatga ttttcaagat
      121 ttttatttac tctatttcca atcttccatg acaaaaacac caataatata aatgatgcta
      181 tctttaaagg acttgttata aaaccttcta tatcacttgc cacgtaactt ataactataa
      241 acattacaaa ttttcctgcc aacattgctg gcacaaaatc ttttaaatct tttttaccaa
      301 aagctgatgc gatagtaact aaacaaccgg gcataaatgg acaggcatac gctataaata
      361 ataacttaaa tccctgttta tccatccaac ttattgcttt ttcagtttta ctatttctca
      421 acttattaaa aagcttacta tcattaaatc tgcttattaa taagaataat gaagttgtcc
      481 caagtcctga gccaatccat gataacaaac atccaataaa taatccttgt attgcagcat
      541 ttgctgtaac tatagcaaca agtggtaagg ctggtataaa gctttctata aatgcactta
      601 ataagccgat tattgtggct aacacccaat aattttgagt caaatcaagt agttcttgta
      661 tatactgcat attgcttttc ccctttttta actttaacta ttattatata ctctatatgt
      721 ttattatata ccaatctcaa aaaatgaatc taatattaaa aagtaaataa tctatataag
      781 tttacagtta aggtataatt attttattat atattataac aaaaaaggtg atgtttcaaa
      841 ataaaaatct gaaacatcac ctttttataa cttaaattgt catgtaagtt ataaaaaata
      901 tatattagac ataatattac taaaaatgtc tagtcagagt aaaataaatt acacagtaca
      961 acaaatatta ctatttaatt gagttacttc tttataacat ttaaccttct tcctactctg
     1021 ctcaataatt cattcgatat actacatgag ttatttgcaa cttcagaagc agataattca
     1081 gataaattat ctcttcctat tattattgcc gtattaccaa cttctacatt aggtatatct
     1141 gtaatatcaa cagcaagttg gtccatgcaa attcgaccca ctattgctgc tctacaacca
     1201 tttattatta cgtaactttt tccacaggat aaatttctag gtataccatc ggcatagcca
     1261 atagataata tagcaattac gctatctctg cttgcaataa agactcttcc atatccaaaa
     1321 ctatcatctt tttgtatttt tctaataaga attacttgtg attttaagga taaaacaggt
     1381 cgtaaatcaa gttgcaattt agtcgtatca tttggagaac ttaatatccc gtataatgca
     1441 attccaattc ttacataatt acacttaaga ttgggataat taaaaaaacc ataactactt
     1501 tgaatatgta ctttaggaat ttttatacct ttttttaaca gaaaatcaat taacttataa
     1561 aaacattcaa tttgtttatt tgtaaagtct acatcttcat ctttcagact atctgaaaca
     1621 cacaagtgtg taaatatgcc acatatatct aagtatttta atttaaatgc ctttaaaatt
     1681 gacctcacat cattaacatc aaaacccagt ctgtgcatac ctgtatcaat tttaatgtgt
     1741 gattttatgt tgtatttctg tttgtttaaa gagatagcat gattaaggtc aataacactc
     1801 tgtgtaagat tgtatttatg aagttccttt gcccttattg ggttagtata tccaagaatc
     1861 aatatttctc ctagtattcc atatcgacgt aaatcaatcc cctcatctat tgttgctact
     1921 gcaaatgctt ttacacctat ttggtttgca caagtcgcta cttcaaaagc actatgacca
     1981 taggcattag cttttacaac tgccatcaat tcacaatctt ctggcatagc tttttgtatc
     2041 atctttacat tatgttccaa attatttaaa tcaatttcaa tccaagctct attggtattt
     2101 accttatgtt ctcctttcaa gtcttttttt agaggtttaa gaatcaatat aataattata
     2161 gaagctataa aagatgctat acttaccact atataatgaa taaggctatt gtcaaccaag
     2221 acagtttgta ttccgaatat tttagaaaac atacgaataa caacaatcat cataggatgt
     2281 ataatataaa taattaatga tgatgttcgc aataacctag ctcgagaacc tttcaaaaat
     2341 gttaaggctg taaataagaa atacatacat ggcaataaca taatatacat gctatcatga
     2401 cgctgtaccc ctagtttatg taaaaacatt ccctcacata acataagtaa gaatgatata
     2461 agaaatccaa tcaagctatt tttagttgac atataattag atttattaga tattattcca
     2521 ccaagtatga aaaatacagg tgcaaaaaat ataccatttc gtgtataatc tgatacttca
     2581 aaaatatacc catataaatt ctttaatagt ggtatttttt ctaaaaaacc ataataacta
     2641 tcaccaaaca taccaattac ataaataaca atagtgatta ctagtgagta ctttaaacct
     2701 agtcttttta caagaaagta tgcaatactt gcaccaataa ttgatgcagg tagataccat
     2761 aaatgatata aagtcccatc aaatacaatg tccttaataa tattgggcac taaattattc
     2821 atggaaaaat atccagtgta aatatttaag ggaatataaa ttacaatagc tatcccgtaa
     2881 ataaataata cattttttag aaaagatttc agtttattac aattataatt atatgttgaa
     2941 attagaaaaa agcctgatag cataaaaaag aatggcacag caatacgagc aattatacta
     3001 gttaacacaa agtcagccat tttactatat gataataatg gagatgtatg aattgcaatt
     3061 ataagtaatg cagctataat cctaaaataa tcaagccctg cataagattt attcctaatc
     3121 attcttgttc ctccatttct tagtcctttc tatttctcca aaatgtcaca ataaaaccat
     3181 ccttattgtt acccgatatt tgatatacac attgttcagg tatagagatt ctcacttctt
     3241 ctttagtatg aataggcaca taaaagattt ctataatttt ttctttatct tctattttta
     3301 aacaatttgt atacgaaaat ctcttaatat attctatata ttcttcaaga gcgagattca
     3361 ttttattgat aatctcagaa tgcggatatc ccacataacg aaaatgccat ggttcttgag
     3421 atattcctgt tatttcttct tttttacact gataacgctc tataaaacca taatgtagtg
     3481 ctgccttacg aaactcatta caaataccat tataaggaaa ttcagggcag ataaaatcta
     3541 taacttcttt ctttaaccct aaatcaatag caagccctgt ctgatgttca ctgtgatttg
     3601 gaagtgctac aaacttttta gtaaagtctt ttccattgtc acgcagagaa cttgaatata
     3661 tatcttgttg ttcctctgca gtacgatatc cacttacagg tactatttca tttattccac
     3721 atatactttt taaaatattt tgtaaaatgt ttgtagcttc acgtttcata agaatacttg
     3781 gaaaccttgt atctacagga attaaatcta tatcttcaga tgttttaact ggtaaaaaag
     3841 catttactag aattagattc ccatcataaa ctttatcttt agtcaataag atagtatttg
     3901 tcattttatc ttctttattt ttatctaata aaatattatt catcatttta tgtacacctc
     3961 aattatcttg tccaatattt cttcaaaaga caatccaata cctttcatca tatttggata
     4021 acgactatga gaagtaaagc caggaattgt attgacttca ttaaaaataa tttcaccaga
     4081 tggagttaaa aacatatcta ctctagcaaa tccagaacat ccaagagttc tataaataat
     4141 ttttgctgtc tcttgtattc ttttctcaca atccaaatca attctagctg gcatatgaat
     4201 ttttgaactt ttcaatgtat atttttctgt ataatcaaaa aatccttttg acaattctat
     4261 ttcatctact cttccaacaa gtaagtcatc gtttcctaaa actgcacacc ctacctcaaa
     4321 accatcaata ttttcctcaa ttattacttc attatcatgt tcaaaagcca agtctactgc
     4381 tacttgtaac tcttcttttt tataaatttt actaatacca aatgatgaac ctgcacgcac
     4441 aggttttata aataatggat aagttaagtc ttttgacagt tcagccacat taaatttatc
     4501 tgagtatttt agtaatattg attttggtac tttaatacct tctgaatgaa caagtttatg
     4561 tgatttgtct ttgtccatac ataatgctga agaaagagta ccacatccta taacctgtat
     4621 acctgccaat tcaaataacc cctgcactgt tccatcttct ccattttttc catgcagtac
     4681 aggaaacaca aaatcaagtt tagtgtattt aatatcattt tcacatgatt caatagcact
     4741 atttggaata tgattctgag aaacagcatc aggaattaaa aattcaataa taccattttt
     4801 gacacggttc tgcgaaacaa taactggaat tatatcagta tccctttcaa accaagtgtt
     4861 attctggata ttattataat ctccaatata atgataccaa ttaccctccc ttgtaattcc
     4921 aattggtata atatcatatt tttccgtatt caaattctca agtactgcaa aagctgattg
     4981 taatgataca tcatattctg ttgagtttcc accaaatata actgcaattt ttttattatt
     5041 catctttgat tctcctttgt tgatttattt atttttatac aatctacaga aacatgttaa
     5101 aatacttttt ctatttcaaa tccaatcaac cttcacttac aattgcatct gttccacata
     5161 atatattttc acatttttct attgatataa ttactgtatc aaaataaaag cactcttgtt
     5221 ttgatacagt aattatatca ataacaagag tgctctttat ttatttatcc taaagaattt
     5281 cctatttttt tatacttaaa ttcttacaaa tttcctacaa tcggaataat aaccttaaag
     5341 ataattatat tgttctcgct atgagctgtt attgtcccgt tatgaagctc cactatttct
     5401 ttagctatag caagtcccaa cccagctcta ccactgtttg aattacgaga tgtatccaac
     5461 ctgtaaaatt gttcaaaaat acgctcaagt ttttcctctg gaatcgtatt gctatgattt
     5521 ataaatttaa gctttatata atcatcctcc tgtacagctg ttatttcaat agtagtatct
     5581 tcaaaactat aataaacagc atttcgtaat aagttatcaa atacacgttg cattttatta
     5641 acatcacacc ttatcatcat atttggtgta acattcagtg tatattttaa attcttactc
     5701 atcaaagtag gtttaaactc atatgttagt tgttcaagca tacgagttaa gcttactgta
     5761 ctgtattcaa gtgatatatt tgataaatta aaccttgtaa tttcaaagaa ttcattaatt
     5821 aaatcttcaa gacgctctgc cttatccaac gaaatagata aatatttttt tcgcagttct
     5881 tcagaaatct gactttcatc tcgaagtagt gtcagatacc ctataactga tgtaagtggt
     5941 gtttttaaat catgtgcaag ataaacaata agttcattct tacgttgttc tgccatttgt
     6001 gtctcaaatt tacgttgttc aagtatatgt ttaattgagt ttattttctt ttctattgcc
     6061 aataactctg gagataaagc cacttctcca acatcttctt tgataagaga gtcaatacct
     6121 tgatttattt cactaaaata ttttgtaaac caatttaaat aaacccggaa tattataaaa
     6181 aacacaatca ccatagctag tatcatgaag agctccatgc tattacggaa aatctgctga
     6241 tataaattca atgcagcttt atattccata cgaaaagcat tttcaaagaa tgatactatc
     6301 cagatagaaa acttaccata caatataaaa gaatataaaa tccatatgaa aataatggct
     6361 ataaatgcca ttaaagctgt tctcataaaa attttatttc tcacttgagt aaagtcagga
     6421 ttgactttaa tatttttact tttcaattgt ataacctact ccccatacag tttttataaa
     6481 tttcgggttt ttagaaggtt catgaatttt ttcacgtaat cgccctatat gagccataac
     6541 tgtgttatta ttatcaagat atttttctcc ccatacagct tcaaacaatt cttcagaagg
     6601 aaccactttt ccttgatgtt cacataaata ccaaagaata gaaaattcaa tcggtgttaa
     6661 tgctacttct ttcccaaata aactacattt atgtgtttct ttattgataa tcaatccttt
     6721 aatatcatac tcattaacaa ttatcgactg ttgttcataa gaattattat atcgcatata
     6781 tcttcttaat tgtgtcttta ctcttgcaac aacttccaaa gggttaaatg gctttgttat
     6841 ataatcatca gcacctatgg ttaaacccat tatcttgtct atatcttcaa ttttagctgt
     6901 taacataatg ataggataat agtatttttc tcgaattttc tggcaaatat gaaatccatc
     6961 aatatcagga agcataatat ctaaaatagc catatccaaa tttttagatt caatacattc
     7021 tagagcttcc ttacctgtat aaaacttgta tactgtaaaa ccttcatttt ttaaatatac
     7081 ttcaattaaa tctgctattt ctttttcatc atccacaatc aaaatatttt cgttcataga
     7141 ttatttttca ctctccttat tatcgaggtc atcatttata atattggtaa atttacatat
     7201 caaataatta caagcatatt tgtaactaat ttttataaga aaagaactac tacatcagaa
     7261 tgtgttgatg ctactattgg aacacaaaaa ctgagattaa ttgttattat aaaaactccc
     7321 ctaacacgtc cttattatcg cataacaggt attaactttc aataaaaata tctatcagtc
     7381 aaacacaatt caatttatta taccagagac aatccatata aaaaacaatt aaacctatgt
     7441 atgtaaccac agacattact aaaatttcca tgttatttta tataaaaatt caaaaaagga
     7501 gttgaattta aaatttgatt agaaaaaaat caattttgag acaactcctt tttattttaa
     7561 tgtatttaaa ttatagtata tcagccttca taatatcctc aaaatcacct ttttcagata
     7621 ttaatcttat caatttaaca gtacagctta aatcatttat aagtatagct ccaactaatg
     7681 aaccatcttt cataaataat tttttatata ttctttgacc ttcatctttt gatgaaattt
     7741 ctttatcaaa attttgtata ttaccaatac taaatacttt tacattcatt gcatctaatg
     7801 aaactggata tacttcactt tcaaatgtca aattatctcc acttgcattt gcccctgcta
     7861 tttttcccat ttctactgaa gatggccata ttgctaaact aatatttccc acttgtgcaa
     7921 catctccaca agcatatata tctttaacat ttgtctgtaa tgtcttatct acaacaatac
     7981 ctctatcaat ttttacagat gtatcctgaa ccatttgagt gtttgctctt acaccaattg
     8041 aaaatactac catatcacag tcgacactat ctccatcttt aaatacaact ttttgagctt
     8101 ttccatcacc ttcaatacca tcaactgcta cacccaatct tacatctatt cctgtatctc
     8161 ttacacattg ttctaatatt ttactacctt ctttatcaag ttgttttgat agtatgctat
     8221 ccatggcttc aactactact acttctaatc cttttagtct aaattcccat gcagcttcaa
     8281 gtcccaataa accgccacca atcactacaa ctttcttaga tttttctagt gcttctttta
     8341 cactatataa atcttttata tttctaagag taaacacatt ttctaaatca tggcccttaa
     8401 ttggtggtat aaagtttcta cttcctgtag ctattacaag cttatcaaat ttaatagctc
     8461 catcattcac tataatagtt ttattcactt catctaattt atctacattc gttcctaata
     8521 ttactactat attattttta tcataccaat ctttatcttc catgtagaaa tctgaaccaa
     8581 gttcttcatt tataccatca gaaagtgcag gtctatagta agtcaactca tcttcattag
     8641 aaatcatagt tatcttacaa gttttatttc tttttcttat agcatctgct gcataaaaac
     8701 cagctgctcc atttcctact attacgaaat attcatcagt atcttttcta aaagtaactt
     8761 catcttcttc aacttctata aattggtctt ccttagctcc acatacaggg catacactag
     8821 gagcactatc accttcaaaa acttcattac aaattacaca tttaaatttc ttagttttct
     8881 ttttacttcc aaaagtagaa tttaacttat ctaggaattt acctgtataa gaatataatt
     8941 tatcaatctc ttcatttgat ggtttaaagt taacagcaaa aggttttact gctgtcattc
     9001 taagctgact tattctctcc atagcatttt ctataccttc tccactccat ccatatgaac
     9061 caaatactga agcaactttt ccaccatgaa gaactgggtt taacgatatt aatatatccc
     9121 atataggctt taacgcatct cctaatattg ttggtgttcc aaataataca ccttgtgata
     9181 ctgctatttt tgccaaaata tcttcttgct tatgctctat tacatcaaat aaatttactt
     9241 tatagttgct atttttttca atatatgctt ttatagcttc tgccataatc tttgtatatc
     9301 catgtgcaga aacataacaa attgtaactt ctttttctaa ctttatttgt tcatttacac
     9361 tccagttgta ataaaggtct ataatttttc taggattttc agttaatact ggaccatgac
     9421 ctggacatac agtatctata tctaaatctt ttatcttttc tatagcagtt accatagatg
     9481 gtttaaatgg acccattata caatcataat agtatcttaa agcatccaag taatcctttt
     9541 cttcattttc atcaagtgta tttactatct tatcattact atagtgacta ccaaatgaat
     9601 cacatgttac aagagtttta tcctctttta tatatgtgta tatggtatct ggccaatgaa
     9661 gcataggtac agagaaaaat tctaaagttt tgtccccaat tgataatgta tctccatctg
     9721 taactgcaac atattcaaag tctttgttta cgatttcctt aagatattta attgctgttt
     9781 ctgaagctac tacttttgca tttttagcta aatccaaaag cttttcaacg cttcctgcat
     9841 ggtctggttc agtatgactt acaacaatat agtctatttt ttcaaaatct atatttaaat
     9901 catttaatct ttcaatataa ttgtcaaaat gcttatcttt aactgtctca aataaaactg
     9961 ttttttctgt tccttttaat acatatgaat tataagtagt tccataaggt gtatacataa
    10021 ttatgtcaaa tactcttaaa tcagggtcaa gcgaacctac ccaatataaa ttttctttta
    10081 tttttagtga tttcaatgac ataccgtact cctcctaaaa tagttattta gtctctttat
    10141 accctgcttg ttatatttta aacaaaattt attcaaaaaa gaaattattt taaagatagt
    10201 ttaaatcttc taatcttctt tttctacctt caatacagtt ccctttttag aatctacttt
    10261 tatgtcatat tcatttttat ctttatctat tcttatttta taaaaagtaa catctaaatc
    10321 tttgtctaaa ctccattctt taactattcc attttgttct tttaatgcaa tttccatagc
    10381 ttgttgaggt gtcattatgt catttaaatc taatttttct tttctagcaa ctccatttaa
    10441 atcttctgaa tccaactttt ctgttttatc ttctaataca tcttttgtat tagcatcaat
    10501 tttcatctta tattcattat tatcatctac accttccaca gtataaacaa atttatttaa
    10561 atctctctca agacttaaat ctactatatc agcatcctta tatttatctt taaatatgtt
    10621 tattgcatct gtgtatggca ctttaaaatc ttgttccatt aaattttcat tatttttttc
    10681 atcttttaaa gcgttatttg atgcatttgc ctcctttttt gtattatctt gtgcttgtcc
    10741 tgaatcctta gttggactcc cactacatgc agttaataaa cccgctataa gcatacttac
    10801 cactaataaa tacgtttttt tacacataaa aaccctcctt aaatatatta atttattcta
    10861 tattatgccc tctaaaccct ttatttaccc ttttatatta aaaattcctc ctattttatc
    10921 aattttaaaa ctttatattt actaaaatta aagttacata aaaattatac tgtctcaaaa
    10981 tggaaacaag cctctttttc attgttgaaa taataaaaat atattttatt tcgtacaaaa
    11041 agagtgcctc atgaactaaa aagttcattt tgagacactc ccattttttt atatttttac
    11101 ttacaaaata acagtactta ttaatttttt acaaacctta taagtatatc tatatgatat
    11161 tcttaaattt aaacactata ttaaaaaatt aaattttgtt taattctttt accgttaaat
    11221 ccattttagg atatggaatc gtaattcctt cttcgtcaaa cctaacttta acctcttcta
    11281 gtaaatcaag ataaattgtc caataatctt ctgtcttgca ccaaactcta gttgcaaatt
    11341 taatagcatt atctccatgt tctactactc ctataaatgg ttctggttca tttattatta
    11401 atttatgatt attaacaata tctttaagta ctcttctaac atgtagtata tcatcttcat
    11461 accctacact aaaagtcaaa tcaactcgtc tagtattctt tgctgaatag tttattagac
    11521 tatcatttga tacagaacca ttaggtataa gtatctgttt attatctgga gtaaccaatt
    11581 gtgtataaaa aagacctatt tgttcaaccg ttcctccatg acctgctgct tctacgtaat
    11641 ctcctacttt aaaaggtcta atcaatagta taataaatcc tccagcaaaa tttgatagac
    11701 taccttgtaa agccaaacct attgctaccc ctgctgatgc tatcacagca gcaaaactgg
    11761 acaaactcat tccccaatat tccagaacaa tgattataac ggcaacttta agtacactca
    11821 aaagaagaga ctttaaaaac tttcttaaag ttaaatcaat atctctttta ctaagtaata
    11881 acataacatg gtttacaaat ttttttatta ttttaaatcc aatagaaagt ataagtatgc
    11941 ctattataag ttttactccg cttgtagtag cccattctag cagactttcc attatacctc
    12001 ccatatccat cttattaata tctaaattat ttaaatctaa cttgcttata tcatctattt
    12061 tcgtcgtatc cataaaaaac tccttacctt atattttaga accaatatta tagtttaaag
    12121 ttcttatttt ctatttaata taatacaata ccatttgtac atagtaaaca agatttatat
    12181 tccaattata catattagga aaatgcaaac tatttgttat atatatgcaa aatataatac
    12241 taaattaaac tattaataaa acactatata gccttaaaat catcattttt tacccctata
    12301 tgagatgata ctatagttat aatacttagt actataaata ctacaaaacc ttttttatat
    12361 ccatcaacta tattatatga atttacttgt aaagagatag ctataatcaa attaataatt
    12421 ccacttccta taaattcaaa agtattaata acactagttg ccagtccaga ataaatttct
    12481 ttatttttat acttggcatc attaaaaacc tgtagatgtg acataactat acatcccata
    12541 ataaagaatg atattggtaa aactataatt ggtggtttta cctggtatac aactacaatt
    12601 acaatccata taaacaaatc tatcattgct ccaaatttta ttatattaaa tttgctagac
    12661 cttattttag caaatacaaa atccataatt attgaaccaa atataaatcc atatgtaaaa
    12721 aatgatacta taaacgcaga aaaactctta ctcacaccat aaacgtccat tatgtatctt
    12781 actccccaca agcttataaa tgcacttgta agaccaacaa aagagaacat tatcattgaa
    12841 ttataccatg ttgatttgtt ctttataaca gacttaatac catcaacaat attaaccttc
    12901 tctgactttt catagggttc agtatctaca ttgaatccat actcttttgg tgtatctcta
    12961 acaataatat acataaaaca tccaattaca acaccaatta taccgattaa taaaaatgaa
    13021 tttctccaac caacaagttc agacaaaaac acaagtggaa aagtagcaag aactccacct
    13081 aaatttccta caaatgaaaa tttagctgtt gcagaagcaa attctgactt attaaaccat
    13141 ctcccttgaa tttttaaaat acaaagtaat attactgatg ttcctgcccc tacaattaca
    13201 cgagaaatat atgctaattc tatactttga atcaaaccaa ataaaattga ccctaaactt
    13261 gccattatta ttccaagact gctaattttt cttgctccat atttatcaat caaaattcct
    13321 gcaggtattt gcataaatgc atatgcatat aggcaaaagg atgctatatt tgaaatttgt
    13381 atagaattaa aacctaactc atttgctaaa ttgtctgaaa caacagcagt tgacatcctt
    13441 aaaaataata ctaaaacaaa tgaaaatgta attatacccc aaacactcca cttagccttt
    13501 gtataacttt tcatatcttc tccctcaaat ccatatattt ttaaattttc ttgatataac
    13561 ttatataaag tgtatagtta ttcttacaaa tttttacacg caatatcatt atacaacata
    13621 tgtatatatt taactagaaa taaaacacta ttcatattat atacatgaat taactacata
    13681 aataataaga gccatctttt aacattactc aaaagatagc tccatataaa aacatttatt
    13741 ctaaatttaa ttttttatct aatataaatt ttgtagcgaa aaataacgct attgcaacta
    13801 caagactacc aagtattgca agcataaaac taggactctg ataaagatga tataccatat
    13861 ttgtatcttc atttggtaag atgcttccaa ctgcatctcc aacaattgat attactatat
    13921 ttatagctat aaaagaagca aatgccacta tgtttctatg tttattgaat tttggcaatt
    13981 gtcccattga gattgatgta tataataaca aaataaatga tgaatatgaa ataaacatta
    14041 ctactactaa taataaaata tctaaaaagt tttcagaaat ccatttaaaa ctagtgttaa
    14101 acaaatctaa aatttcaggt agtaaaattc cagaagtacc aaaaagcata agaactccaa
    14161 aagtgaatac tgcaactata aaactaagta cagcgtatat aagtgctgtt atacatttag
    14221 ataatataag tgaactagtg ctaacaggaa gagtaaacat taaataacct tcatccccca
    14281 aaagattttt tctaaacctt tgaattgtaa ctacaatagt taaaactcct aatgccataa
    14341 aaagacttgt taatactagt attcctattc cctgtacctg aagtatattt gtattcataa
    14401 atattccatt aaatacagct actatcaata tggctatata taacggtata aatattcttc
    14461 cacttgcttt taactcatat tttaataatt tacctaacat ttaaattcct ccctaaataa
    14521 tgcatctata gattttcctt tttcttctct tattgcttca acgtctcctt gtaacactat
    14581 ttctcctttt gatatgaata ttatctcatc acatatgttt tctatttcac ttattaagtg
    14641 ggtagcaatc agtaaagtac tatcttcaga ataatttttt aatatagtct taagtatata
    14701 tgaccttgca gctgggtcaa caccacctat aggttcatcc aatatatata tgctcgcatt
    14761 tcttgacatt actaaaataa gttgaacttt ttctttagtt cctttagaca ttgtctttag
    14821 cttttcattt acatcaatat ctaagctttt aatcatctca tttgctcttc taacatcaaa
    14881 atcactataa aaatcataga aaaacttaag taaatctgat accttcatcc aatcatttaa
    14941 ataagttctt tctggtaaat atgaaactat ttttttagta tctatggatg gtttcattcc
    15001 atttatcata atttcaccat tatcaggctg taataatcca ttcattagct taatcattgt
    15061 acttttgcca cttccattag gtcctaacag cccgactatt tttccctttg gtatatttaa
    15121 atcaatattt tttaaaacat tcttagttcc ataactcttg tttacacctt taaattcaac
    15181 tattccatct tgagaaacat tgcttatatt tttatccatt ttataattct cctttcagat
    15241 tttctgtaat aagttttagc atttcatctc tatcatatcc aagcttttct aaggttactt
    15301 ttaatgtatt tatctccaca ttagctattt cctctttcat agcttttatt ttttccttat
    15361 catctgttac aaatcttcct ttagttctct gactataaac taaaccttgt ctttcaagtt
    15421 ctgttagagc cttttgcatt gtatttggat taacttccgc atcttctgct agtgctctca
    15481 ctgtttctaa tttagatcct attttaattt cccctgaaat tatttttaac tttaaatgtt
    15541 ctactaattg tatatatata ggtttgttat tatcaagttc ccattccatg ttcatgcctc
    15601 cttattatat tattgtatta agttgttaat acaataatat aataatacaa agtatatgtc
    15661 aattactttt tagtaaaagt ataaaaaatt ttacaaaaaa aatgaacact acattagagt
    15721 ccatttttaa cacaatttat taaattcttt atattacaga ttaattaccc tcttaccaag
    15781 ttagatacaa ttaaatgttc aaattcttca attggcattg gctttccaaa caaatatcct
    15841 tgtgccatat cacactttac ttcttttaaa agtttaaact gttcttctgt ctcaactcct
    15901 tctgatatga ctttcatacc caactctttt gccatttcta ttactttagt aacaattatt
    15961 ttgcttcttt tactattata agtttccata ataaattttt ggtcaagttt taagatatcc
    16021 ataggaattt cttttaacat atttaatgat gagtaccctg aaccaaaatc atccatagat
    16081 attaaaaatc ctattttttt taaattattc attatatcaa ttaaaatatc aaaattatca
    16141 aatatgatac tctccgttaa ttcaagttct atatactttg caggtatatt gtatttatca
    16201 attatatttt tgtatgtttc tataaaatta ttgcaatata aatgtatcct tgaaacatta
    16261 actgaaacag taactggatt tattcctgca tcaatccatt ttctaaggta tatacatact
    16321 tcttcaagca catataaatc taaatttata acaaaaccgt ttctttcaaa aaatgggata
    16381 aattcatttg gaggtattag ccctttatca ggacgtttcc acctaacaag tgcttctgaa
    16441 ccttgtatct ctcctgtatt aagttctatc ttaggttgta agtatataat aaattcttta
    16501 ttttctagac ttgaaaacat tgtattttcc atctcttttt cttttatctc ttggtcatga
    16561 atcttattat cataaaatgc atatgaactt ttatgtcctc cctttgccat tttatgaggt
    16621 atattagccc tatctatgat tgagttgata tctctatctt ctggaagtat aaaatagatt
    16681 ccacaactta aaactaactt atatttttct tcttgattat tattaaacaa gtctaattct
    16741 tcaaatatag tttttaacct ctttgttata tcttcttctt ctatatattt cataattata
    16801 ataaaattat ccgcagatat tcttgcaaac atttcctctt caaatatatt gctcttaata
    16861 atctttgtta aattacgcaa aatttcattc ccaacttcat atccaaataa atcatttata
    16921 tatttaaatt tatcaatatc tatatataat aaagcatact ttacttgagt atttttacat
    16981 agtattttgt tagcatcaat tacaaattta tctatagtat ttgctcctgt aagattatct
    17041 gtatatgcta tcctttgcaa tttaatttta aaatttaatt tatcatatat tatatagaat
    17101 ataagtatta cacatattat agccataata agtattattt gtattgcatg tttgctaata
    17161 agctgattat tagacacaat agactcaaag ttatttatgg agttgttttt atagagtttg
    17221 cataaagaat agactggtgt tgtgtatgta atttgtaaaa ttatacaaat aaataaaaaa
    17281 aacatcaatt ttttttgtaa attcctcata aaaatttcct cccattaaaa cacctctatt
    17341 ttattagcaa ataagtacat ctcaaaagtt tccatttctt gtaaaatgga tagtttatat
    17401 ctactttaat tttgtaaaat tttctgctac tataattata gaatgtagca tattcattgt
    17461 cttaactatt gatttatata tttcatatag gtataataga aaataaattg gattattttg
    17521 gcatataatt at
//
LOCUS       DAYATN010000073        17400 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.073, whole genome shotgun sequence.
ACCESSION   DAYATN010000073 DAYATN010000000
VERSION     DAYATN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.073"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     regulatory      68..153
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            277..6240
                     /locus_tag="ACGI8A_002312"
     CDS             277..6240
                     /locus_tag="ACGI8A_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HFH0180308.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSDSESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDEQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSKSTLE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELARYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDANTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     gene            6450..8330
                     /locus_tag="ACGI8A_002313"
     CDS             6450..8330
                     /locus_tag="ACGI8A_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HFH0180309.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKVTINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGSGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGDDRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNDKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            8697..10298
                     /locus_tag="ACGI8A_002314"
     CDS             8697..10298
                     /locus_tag="ACGI8A_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HFH0180310.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVIQQCISASKSNGQPPAITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDNKVFEHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKSVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            10517..12115
                     /locus_tag="ACGI8A_002315"
     CDS             10517..12115
                     /locus_tag="ACGI8A_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFH0180311.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVADQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNNLKPNIEGTVDTSKAGTYVLNVSAFDNFGQKCEINVNVKVIINTSTKDPNS
                     YEFKAMVSNEMYNLVNSYRKEKGKKSLRELDSLAGMANAWSKYMDEKKIFAHEIDGKN
                     AAEVFSGFGMRTDENIAYLPMNVKSVYTSKDAKEIAKSVFDLWKKSSKYNDNMLKEEF
                     YSFGFGMYVSNQREVNATMEFLNS"
     gene            12408..14588
                     /gene="slpA"
                     /locus_tag="ACGI8A_002316"
     CDS             12408..14588
                     /gene="slpA"
                     /locus_tag="ACGI8A_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HFH0180312.1"
                     /translation="MNKKNLAVVMSAVTVIGSAAPVFAAAEDSFPNGNTTVSSSKYSD
                     IERILKKYVADGVTGITVNFFDKKGNPKGVSISSLEASQISYVKDEIKDLKAGEYAKI
                     SVKITDGTEKKYDDTLLKELDSNAVVTKVGTATTVKIVDQDGNSIENDAKLKLQKYDT
                     TTSAYVDGATKDEVLAATKAAVKTSKFTDKTGNNATGTVAITATYADAVAGAIKLKYD
                     TQAAVAPTLDTAVASLDGTKEIYDFSKPVITVNTTDGTSKLSFEKTGEKVGAVESNVT
                     VIAASDETVTISGDAKEKAEALAKKYVFKDTELEDAYKTVTASDFEKTDNDYYEVVLY
                     PTGKRLNTASTYASSNYKPELPTSDRVDTPAIITLRSTNKNNLKSALDELRTYNNSYS
                     NNSVLAGDDRIETAIEISKDSYNADGGIKGDYVEANEVVLVGSQSIVDGLVASPLAAV
                     KDAPLLLTSKDKLDSSVKSEIKRVMGLDDKTGITSKKTVYIAGGENSVSKEVANELKD
                     MGLKVERLSGDDRYATSLEIADEIGLNHNKVFVVGGTGLADAMSIASVASNKEMPIVV
                     VDGKGKDLSTDAKDFIGSAYVDIIGGKSSVSEDMEDAIDDATGKSPERVSGDDRQDTN
                     AEVIKTYFEKDNSDSVISTGVKNFYVAKDGSTKEDQLVDALAIAAVAGHNEAPIVLAT
                     DSLSSDQSVAISKVTNSDDSKKLTQVGKGIADSVIKRIKDLLEL"
     gene            14824..17169
                     /gene="secA"
                     /locus_tag="ACGI8A_002317"
     CDS             14824..17169
                     /gene="secA"
                     /locus_tag="ACGI8A_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFH0180313.1"
                     /translation="MSVLDTILDKADEQEIRRLNVIVDKIESLEKDIENMSDDELKKM
                     TDLFRDRLKKGETLDSLLPEAFAVAREASKRVLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVVTVNDYLAQRDKELMQPVYEFLGMSVGVIIADLI
                     PEVRKVQYNCDITYGTNNEFGFDYLKDNMVTKKFDKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGEGDEDSKLYELANSFIKTIKEEDYELDRKDKTVALKESGIDKAESFFGIKN
                     LTDIENIELYHHINQALRGHKLMEKDVDYVVVNQEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKDESKTMATITFQNFFRLYNKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPIIRADLHDKVFRTEKEKYQAVVEEVIRIHKTKQPILIGTVSVEKSEMLSGMLKK
                     EGIKHQVLNAKENDKEALIVSKAGKLGSVTIATNMAGRGTDIALGAGDKEEESRVKEL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKSIEKLMKR
                     PNSNKNNSIESKSLTKAIERAQKGVEGKNFELRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQVDIQNMVKDIIKEAAAEYLTKRKRDYYGYFKHLYNTFMPADTLLIPDLDNKS
                     IEDIIDSTYEISKRVYDLKKMVVGLDKITEMEKIVLLKVVDQYWVEHIDAMEQLKQYV
                     GFKSYAQKDPFKEYAMEGYEMFESLNKNIRETTIQYLYKFN"
BASE COUNT         7252 a         1879 c         3306 g         4963 t
ORIGIN      
        1 ataatttaat ctaaaaaaat tgtattaaaa agtttaaata tagtataaaa tgggtaagaa
       61 agtagtgaaa attaaatggc aaaattaggg aaatctaatg acgcaaaact atagggatta
      121 aagcttttaa agccatatca gccagttgct aaaaagagta aagtcttttt attttataat
      181 taagattctc tttgagtgtc tttttttatt gtctgaaact tatagtattt aaattcatga
      241 aaaattagat atttattaag ggaggattgt tatttcatga aaaaggcaat atcttgtgta
      301 ctagcagtat ctatgtgtag tgcaccacta aacgtttttg cagaacctat attagagggg
      361 aagctaaggg cagttgaaaa atcagttaca gaaaagttaa gggggagtct tgaagtagat
      421 ttaaattttt cactgccaat aaaaaatagc gattcagaaa gtatgactaa tatgcagtta
      481 cgattaaaag atgatagtaa caatagtgga ataataaagc ttggagaaaa agattcagga
      541 acagtaaatg ttgggacaga taatatagaa tatactataa aaaaattatc tgcaagtaga
      601 agtgaaataa aaaataaaga tgagaatata tcatattata atattgtatt taataattta
      661 cctgttggaa aatacaatgt agaagtctct ggagatggat ttaagaacaa agaaattaaa
      721 gacattgata tatcaagtta ttcacaaaga gtcttattaa gcaatagagc aagtgtagat
      781 aataaaaata aacctttaaa ttatgaattg ttcttaatgg gtgatgtaaa tggagacggt
      841 aaagtagata agagtgatta taataaggtt ttagagaata tagattcaaa taaaagagaa
      901 tttgatttaa atagagatgg aaaggtagat attgttgact tagattatgt acagaaaaat
      961 ttaggacaaa gtgaagatgc aaaaagccta gaaagtatag tatctaccaa tcctatagta
     1021 gatacaagta aggttgaatt aaaaggaaat agtgatgtag atattaatgg taatgtagaa
     1081 aatttattta gtggagaaaa taatggagtt acaatatctt caaaaaatga tgaaatatct
     1141 gaggaaaaac cagcagtaat ggatatagct ttatcagaag cacttaatat ggaagtcatc
     1201 aatataaaag ctccagaagg tactgctcca gcaaaaggaa tagttgaggt tactgacaaa
     1261 gatggtaatg ttcaacagat tccttatgaa aacaaagaat atatgaaaaa cttaaaagat
     1321 gaggagcaac aagaggaagt aaataacgaa caagaagaag taaatcaaga agaagtaaat
     1381 aataaagatg aaaatataaa ccagactgat aataactcaa ataataaaga tgaaaataat
     1441 gatgaacaac aggaaaatag cgataaacaa gaagtaaact ctaataccaa tcaagataaa
     1501 aataatgata aagaaaattc atcaatggat aaacctaatt cagatgaaga tactccaaaa
     1561 gaagagggta atatcagtaa agatgataac tctacaacat ctacatatag taagtcaaca
     1621 ttagaaacat atgcagatgt tcaaaatagt gatatagtaa taaatcttgg taaacaagta
     1681 gcagtaaaaa agataactat aaaaataaca gctacaacag caaacgatag aaatcttgca
     1741 gaaatatcaa aagttgaatt tttaaacaat gtatataaag aaataccaaa gccagagatg
     1801 aatataccta aaataaataa aatatcgact agcactgctg ttggaaatga aaaagtatct
     1861 ttttattgga ataatgaagt aaatgtatca gcatacgaac ttatactaga taaaattgat
     1921 agtaagggaa aagtattatc tactaaaaaa ttacaaacca gtaaaaatac aatagaagtt
     1981 agagatatag aagcatatgg actatatagg gttagcatac aatctttaaa tggagattgg
     2041 tctagtggtt ataaagatgc aacaccagaa aacttagacg gagtaccaga aaatgtagat
     2101 tcaaactata aacctataca atttagccca gatagtatat tagaatttca agttgttcca
     2161 gacacaaaac cagaaccacc agaaggtata aatgttaaag gtaaatttaa atctcttgat
     2221 gtaagctgga aaaatcataa acaagctaaa gattttgact tgtactataa agaaaaaggc
     2281 tctaatgggt cttggataaa atacaatgat aagcttattc gtgggacaag ttcaaagata
     2341 gaaggtctta aggataatac aacatatgaa gttagaatga cagctactaa ccacttggga
     2401 actagtggaa tgtctaaaac atatataggt acaactgaaa gcatggatgc accaacaaca
     2461 cctaactata gattaattaa tactcctaaa gagaatggtg gatttggaat accaacaaat
     2521 cacattgtag atgttactta tccagcagga tatgacaaaa gtgaatatag tgataaaaat
     2581 ccatttgata aattcaatgt agtagatggg gactttacaa cacattggac attcccaaca
     2641 tataatgcag ctgagggaac gaatagagga ccaataataa cttttgataa atcttatact
     2701 atggatactt ttatggctac acctcgtttt gatgtttctg ataagacaat aaatgacttt
     2761 gaggtacgat attgggatga aaatggtcaa ttacacaatc tgggggatgt tccttttgaa
     2821 acaaagacag accctgtaaa taataataag tatgtaatgg ttagattaaa agaaccaata
     2881 actgctacca agatacaagt aagtgtttgt tcatcctatt catataagtc taatatatca
     2941 gaacttaagt tttatgagta tgacagtata gaagatgatg taagaaatct atttatggat
     3001 gacctacaag ttgaattaaa agaagatgta actcaagaaa aaataactga attaaaagat
     3061 agattaaata ctccagataa agctagtgga gaatatcatc catataagac agttattgag
     3121 agagaattaa aattagctca agacttatat aatgatagag ccacacttac tgatgtaact
     3181 actgtaaatc aagaaataaa taaattagga aaatataaag ataaaaacgg acagtatact
     3241 gtaaattcca ataatttagg tatgcaaaat gactggcagg cattaggagt agctgctaga
     3301 gcaggagatg aaatcacagt atatgttgga agtaaaagtg gaaaaactcc taagttgata
     3361 tatacacagt actatggaga atctggtgca tataagagtg gagaaataaa tcttaacact
     3421 ggaaaaaatg taattcaatt aagcaaactt catagtttag atatagaatg tggaggaagt
     3481 ttatacataa gatacgctga agatactcca agtggaggag atataaaagt acgtgtagca
     3541 ggagctacta agataccaca ccttaatcta aatggaacaa taaatgataa gagtcctgcg
     3601 ggagtttctg aatctaagaa aaagataaaa gcatatatag aagaattagc tagatataaa
     3661 gatgctgtaa aaggagaacc atattatcca ggaacaaatg ttggtaattc atatggatat
     3721 tcagaaaaaa ctggtgtgtt aaacactaca gatattgaaa gtgataaagt aacattaaat
     3781 gttcctgcaa cagctgtata tcaagggata tcaaaaggaa atgctgattt aaatacacaa
     3841 gtagatagat tatataatag tatgcttgca tgggaacaaa taatggattt agtttactca
     3901 gaaagaggag tatttaaaac acaagattta gatggtaatg gaactgtaga tgataatgaa
     3961 tttaacttaa ctaaaaatga tatggctcca aaatctcgta tgaacataaa ataccaaaga
     4021 atgtttattg gagcatttat gtatgcttct ggattacatg taggagtaga atatggttca
     4081 gttccagggc ttatgaatgg agtacctttc caaatagatg atagtggaaa agctacagga
     4141 ggaaacttat ttggatgggg aataggtcat gagataggac atgttacaga tattggaaag
     4201 atgacatata gcgaaactag taacaatgta cttgctttac ttgctcagac ctttgatgat
     4261 aagacacatt caagacttga aggttcaaca atggataaga tatatgagca tgtaacttca
     4321 aatagcttag gtataccaag caatgtattt gaaaggcttg gaatgttatg gcaattacat
     4381 ctagcttatg atgatgattt tacgggttca atgttaaaga ataactcaga tgctgattta
     4441 agtaatgata cattctatgc aaagatttca agaaaataca gagcactttc aagtagtgac
     4501 ccaattaata gtttgccaaa agaccaaatg ctagtagcaa tggcatcaag tgttgttgaa
     4561 aaagatttaa gagattactt taaggcatgg ggagtagaaa ttacaccaga attaaacagt
     4621 ataatggata gtaagaacta cgaaaaagaa tcaagaaaga tacaatatgt aaatgacgaa
     4681 gctagaagaa agattttaaa tgaaaatata gctagtatgg caaaagatac taaagtaagt
     4741 gctagttttt ctgatggaat acaaagtggt tctctagtta aatctaataa aatatctata
     4801 gacctagatg tagataagga taaagataag atactaggat acgaaataat aagaagtgat
     4861 ggaaattatt tagatggtga aaatggaaca caggtaaaat acagaacagt tggatttgtg
     4921 gatgcaaaag gaaatagtaa gactaaattt actgataata tatctcctct taataacaga
     4981 gcatttacat ataaggtagt tgcatatgat tatcacctaa atccaacaga agaatttgaa
     5041 gttggtactg taaaattatc agatgaaggg aaaataaata aatcagcatg gtcattcata
     5101 acaaatactg tttctgacgg tgatgttaga actgaaaatg actcacatgg accattacaa
     5161 aatcctgaaa ttgataatat aaaagataat gatgcaaata ctacatataa aggaaagata
     5221 ataagtaaag cagagtggaa caaagaccct caaaaagacc ctgatataaa tgtagaggaa
     5281 aatccatata taatagtaga catgaaagaa acattaccta tagtaggact aaaatataca
     5341 aaaccagaga cagaagctag gaagttctca ttaaaaggat tatttaactt tagaaagaat
     5401 actagcacaa catacaatcc tttaactaat tacaaaatac aagtaagtaa tgataaaaag
     5461 aactgggaag atgtaagtca aggaactttt gaatatggac aagatttagt tggtggagga
     5521 aaagaccaag ataatgaagc tagagttaca tttaataaag atggtaaact ttggacatac
     5581 caagctagat atgtaaaact aatctcaaat aataagtcta atatagaagt tgcagaatta
     5641 gatataatag gacctccagg tgataatata gaaattggca caatgaataa tgcagacgaa
     5701 actcgtacta atggaattgg aaaattagca gaagattatg tatatcaaag tgatgactct
     5761 agcacaagtg aagatgaaac aggtaagatt ccagcgggtt caatagttat tacaggtgaa
     5821 tacagtggaa atccagcatt taatttacca ctacttatag ataaaaacaa taagactata
     5881 tctggagaag cacttttatt tgcagaagtt ccagaaaaag gtgaactagg tgaaatcagt
     5941 aaaggtacat gggtttacta tatgccaaaa gaaaactttg attctttaag tgataaggtt
     6001 aaggctgaac tatatagata caatgacctt caaggagata ctccagtagg tcaaagattt
     6061 gtaagtgata caatgtatgt acctgtagga gctaagaatt atgatgagtt aaagacaata
     6121 agtcttactg atagtaatag taaagctaga aaagctacaa gaaattctat tgatatcagt
     6181 aataagaagg ttataactgt aagcaataaa gtaaagggta atattgttag agataaatag
     6241 taggtggaac tctaaaaacg taaaaatact aacactaaca ttgtatatct aataagatta
     6301 ggtactagaa tagttttaaa atagtagata ttaaaagtaa agtatataaa tgtaatcata
     6361 aatttagggt aatttataaa ttaaatataa aagctaggtg gataagtaaa tttatccact
     6421 tagttaatga tagataagga cgggtgttaa tgagtaaaag aaaatctttt ataagaacta
     6481 tagcagtatc aactatggca gtagctgtaa ctggtagtgc tacatgtgca tatgcagctc
     6541 cagttttaca aggaacaaaa acgtatgaga aagtaaatac tatagatata tctgtggact
     6601 cagtagaaaa catagtatat tcatttcaag ccagtataaa agtacaaggt gaggttgaag
     6661 tagtagataa tgagcagaaa gaaaagatta cttggtctga taatataaaa tctcagataa
     6721 aaagtggaaa tgcaaatgct acttgtagag cagaatacaa caaatcaagt aatacaacta
     6781 cattagatat atatgttaca tcaaatgaag acttactaga cggaaataga ttgaatatag
     6841 gaagaatatc tgtaaaaaaa tctggaagta actcaaatgc tgactataaa gttttaggaa
     6901 aaggaacaaa tgataaacca gctttaaaaa ttgtaacata taacaacaaa acagtggatt
     6961 atgaaaatat aagctctgat gaaggattaa tatttaccct tattaatgag agtgaagtaa
     7021 aaccgattgg gggaactggt tctagtaaaa atgaccctga aaagtataag gtagagaaaa
     7081 gtgaagcact tgaatattta ttaaacaata ttaggataaa ctattctatt gttagtaaag
     7141 aaacacaaga aagtggttca aatgtaatat taaaacttgg attagcacaa aaaacaacta
     7201 agggaagaaa agttacaata aacaaatatg ttgaagttac acttccaaaa agtttagaat
     7261 acatagttga aaatgaatta tcaaaaccgg atgaattacc accagataat ggtagtggtg
     7321 gaaataatgg tggaggaagt aattcaggcg gctcatctag tggtggaagc tctggaggag
     7381 gaaattcaag tgattctaca tctaatgtca cagtcaaaaa attaaaaggt gatgatagat
     7441 ttgagacagc tatcaaaata tctcaaagtg gttggacaaa atcagataca gttgtaatag
     7501 taaatggaga agataaaagt atggtggatg ggcttacagc tacaccactt gctagtgtaa
     7561 aaaactcacc gattctttta tcatcaaatg ataaacttcc tcaaaaaact gtagaagaat
     7621 tgaaaaggct aaatccatca aaagtagttg tgataggtgg aaacaactca atgccaaatt
     7681 cagttgttga agctatcaaa gctgtaaatt caaaaatatc agtacaaaga ataggtggag
     7741 atacaagata tcaaacttct attaatatag caaaagaaat agataagacg aataatgtaa
     7801 gcaagttgta tataggagca ggaaatggag aagctgattc attatcaata gcatcactgg
     7861 ctggtaaaga aaaaactcca atagtactta cacaaaaaga tggtgtagat aatgaagctg
     7921 aacaatttat taaatcaaat aaggtgtcaa atatatattt tattggtgga gtagaaaaaa
     7981 tatctaacaa ggctattgaa caagttggaa aaatagcaaa caaagatata tcaaacaata
     8041 gggtggcagg tcaaactaga caagagacga atgccaaagt aatagataaa ttctactctc
     8101 aatctaagct agatggagtt gtagttgcaa atcaagataa acttatagat gctttagcag
     8161 ttggaccatt agcagctaaa aacaattcac cagttatact tgctacaaat acgttagata
     8221 aatcacaaga atcgtcatta aaaggtaaaa attcatcaaa gttgtttgaa gtaggtggag
     8281 gaatagcctc atctgtaata gacaagatta agagtttaat tgaaaaataa atattaagta
     8341 aatagaaaat aagaatgtat ttagatataa agttatatat ttaaatgcat tcttatttta
     8401 ttttcattat cttaacaatc taataatatt gaggttaaaa tgtataataa tttgagctat
     8461 attaagttta attttaaata aagcagatat cttttatata tataaatata aattcaattt
     8521 tgaatatgtt taaaaagaag ttaaaaataa ttttttaaat ttaataaaat aaaacactaa
     8581 tatatagacg agatatattc atatcatata tactctataa tatatggaaa ataaagagaa
     8641 aataaatgga aaataatcac aatataaata taaaatataa aaatcatcag ggggatatga
     8701 aaatgaacaa aaaaatatta tcattaggtc tagcagtatc attaatctta gtaaacttca
     8761 aaagtgtaaa tgcatcatca gtagtagaaa aaatatacgg taaagataga tatgaaacag
     8821 cagcaaagat agctgataaa cagacttatg aaacagtaat tttagtaaat acagaaaaat
     8881 cacttgcaga tggattaagt gcaagtggat tatcgggagc tacaaaagct ccaatactat
     8941 ttacacaaca aaataagata ccagcagaca caaatagatg tctaaaaaat atcaaaaaag
     9001 catatataat aggtacagaa gatactataa gtaaatcagt agaaaaagaa ctagattcta
     9061 aaaatataga agtcaaaaga attggtggag aagatagact aaaaacaagc tatttaatag
     9121 ctaaagaaat agcaactata aaaaaagttg ataaagtact actaactaat gcatatagtg
     9181 gagaagcaga tgcaatgagt gtatcatcag tagctactag agatggagct ccaatcatac
     9241 ttacagatgg aaagagtgtg ccttttgatg taaaaaatat tcaatcatat tgtataggtt
     9301 cagaggagat aatgagtaat cctctagtta aaaatacaaa ctcagtaaga atagaaggaa
     9361 ctgaccgatt tgaaactaac aaaaacgtaa tagactactt ttttaatagt gcagatggat
     9421 tctatgtatc agatggatac caattagtag atgcaatagc agctgcacca cttactaaaa
     9481 actctccaat ggtactagta aatgatggaa gcgataaaac tgtattagaa ggagctaaga
     9541 atataacctc tgtaggtgaa atagatgaga aggtaataca acaatgtata agtgcttcaa
     9601 agtcaaatgg acaaccccca gcaattacag ttggaagtac agaggtatat aagggtgaaa
     9661 agtttgacac tggcaagtta aatatagtag ctaaagataa cactgggaag gtattaccaa
     9721 tagaagttga tgggtttata gatacaaata gagtaggtac atacatattg acattaaaag
     9781 ctacagacga atggggaaaa agtacaggaa aaagagtaga aataaaagtg ttggatgata
     9841 aatctcatga ttacaacagc ccagaattta aaaaaatggt atctactgaa atgtataact
     9901 taatcaattc ctatagaaaa gaaaaaggta aagagccttt ggtagtatct agtaagctag
     9961 aaggtatggc aaatgcatgg tctaagtata tgatggataa caaagttttt gaacattata
    10021 tagatggcaa aaatgctcca caagtatttt ctgagtttgg aatgagaagt gaggaaaata
    10081 tagcatatat ttatatagat tctaagagtg ttcaaacaac tcaagatgca aaagacttgg
    10141 caaaggctat atttgaagtg tggaagaaat ctccagaata caatgcaaac atgcttagtg
    10201 atgagtttta tagtactgga tttgggcttt atatattatc tgatggtcaa gtgcatgcta
    10261 ctcaagagtt tttaaatggc aatgaaggta gcttgtaaaa aattccataa atcttgtttt
    10321 tttgttaatt tactgatatt ataaaatgaa aatcacatct aaagtacttt ttgtaatctg
    10381 ttttattatt gctttacagg ttatggtatt ttaggtgttt tttgttttag tagtttataa
    10441 attttgaggg aatcactact gagggtgtaa tttatgatta gctgaattat ataaaaaaat
    10501 tagtaggagg agttcgatga aggtaaataa gagagtatta tcaataggat tgacaatatc
    10561 attaataatg gcaggagcac caaatatcaa tgcactttcg agtatagaaa agatacaagg
    10621 gaaagataga tatgagactt ctgcattgat agcagataaa caaatatatg atacagtcat
    10681 attagtaaat actgataatt ctatagtaga tggattaagt gcaagtggat tatctggagt
    10741 tgtaaaagct cctataatgt tagtgcaaag agataagata ccaacagatg ttgaaaaaag
    10801 actaaaggat gttaagaatg cttatgttat aggtacagag gatacaatag gtaaatctgt
    10861 agaaaatcaa cttaagaata aagggattga agttaaaaga attggtgggg aagatagaat
    10921 aaagactagc tatcttatag ctaaggagat ttcagctatt aaaccagtaa atgatggtga
    10981 taaggttttt cttgtaaatg gatacacagg tgaggctgat gcaatgagtg tatcttctgt
    11041 agctgctaga gatggagttc ctgttatact tactgatgga aagagtatac catttaaggt
    11101 tgatggcgtt caatgttatt ctcttgggtc agaggagata atgagtaatg aacttgtgag
    11161 taagactaat tctgttagga ttgctggtaa agatagattt gaaactaata agaaggttat
    11221 acaacgcttt tataaaggaa caaagaagtt ttatgtttct caagggtata agttagttga
    11281 tgctgtggct ggttctccgt tggctaagga caaacctata gtacttgtta atgatggaag
    11341 tgataagagt gtgcttagag gtgctgatga agttacttct ttaggtggaa tggataagaa
    11401 ggttgctgac caatgtatta gttctgcatc tgataaaaat acaatgccta caatcactgc
    11461 taatgatgta gagatttctg taggggataa gtttgataat agtatgttga atatagttgc
    11521 cactgattat tatggaaata atttgaagcc taatattgag ggaactgtgg ataccagtaa
    11581 ggcaggaacg tatgttttaa atgtaagtgc ttttgataat tttgggcaaa agtgtgagat
    11641 taatgtaaat gtcaaagtaa ttataaacac aagtacaaaa gaccctaata gttatgagtt
    11701 taaggctatg gtatctaatg aaatgtataa tctagttaat tcatatagaa aagaaaaagg
    11761 taagaaaagt ttaagagaat tagattcttt agctggaatg gcaaatgcat ggtctaagta
    11821 tatggatgag aagaagatat ttgctcatga aatagatggg aaaaatgcag ctgaagtatt
    11881 ttctggcttt ggaatgagaa ctgatgaaaa tattgcatat ttacctatga atgttaagtc
    11941 tgtatataca tctaaagatg caaaagagat tgctaagtct gtatttgatt tatggaagaa
    12001 gtcttctaag tataatgata atatgttaaa ggaagagttt tatagttttg gctttggaat
    12061 gtatgtttct aatcaaagag aagttaatgc tacaatggaa tttttaaata gttagagtat
    12121 atattaatat tttatggtag aaaatattaa tatatattga ttaaatcaga taaataaatg
    12181 tattaataaa attaaaattg aaaatttaat ttaaaaaata tgaaatagca aaaaaaaggg
    12241 gaaaaatgtt gaaagaaaat tctattaatt ttgcataaat tattgaaatt ctattttata
    12301 tagtctatca taagagtgtg agaaatattt ggtttatatg aacatggaat tttaatgtac
    12361 ataattaaag agatgtaatt aatataatgt tgggaggaat ttaaggaatg aataagaaaa
    12421 atttggcagt tgtaatgtct gccgttacag taatcggatc agcagcacca gttttcgctg
    12481 cagcagagga tagttttcca aatggaaata ccactgtatc tagtagtaag tatagtgata
    12541 tagaaagaat acttaaaaaa tatgttgcag atggtgtaac aggcataaca gttaactttt
    12601 ttgacaaaaa aggaaatcca aaaggggtat ctatatcaag tttagaagca agtcaaataa
    12661 gttatgttaa agatgaaatt aaagatttaa aagctggaga atatgcaaaa atatctgtaa
    12721 aaattacaga tggaacagaa aaaaaatatg atgatacatt attaaaggaa cttgatagta
    12781 atgctgtagt tactaaagtt ggtactgcaa caacagttaa gattgtagat caagatggaa
    12841 attcaataga aaatgatgct aaattaaagt tacaaaaata tgatacaact acgtcagctt
    12901 atgtagatgg agctactaaa gatgaagttt tagcagctac aaaggcagca gtcaaaactt
    12961 caaaatttac tgataagact ggtaataatg ctacaggaac agtagctatt acagcaacat
    13021 atgctgatgc agtagctggt gcaattaaat taaaatatga tacacaagca gcagtagcac
    13081 ctactttaga tacagcagta gcatctcttg atggtactaa agagatatat gattttagta
    13141 aaccagttat aacggttaat actactgatg gtacatcaaa attaagtttt gaaaaaactg
    13201 gagaaaaagt tggagcagtt gaatcaaatg taacagttat agcagcatca gatgaaacag
    13261 ttacaatatc tggagatgca aaagaaaaag cagaagcttt agctaaaaaa tatgtattta
    13321 aagatactga attagaagat gcatataaga cagtaacagc aagtgatttt gaaaaaactg
    13381 acaatgatta ttatgaggta gttctttatc caacaggtaa aagattaaat acagcttcaa
    13441 catatgcatc atctaactat aagccagaat tacctacaag tgatagagta gatactcctg
    13501 ctataataac attaagatct actaataaga ataacttaaa atcagcttta gatgaattaa
    13561 gaacttataa taattcttat tcaaataatt ctgtattagc tggtgatgat agaatagaga
    13621 ctgcaataga aataagtaaa gactcttata atgcagatgg aggaattaaa ggagattatg
    13681 tagaagctaa tgaggtagta ttagtaggat cacaatcaat agtagatggt ctagtagctt
    13741 cacctttagc tgctgttaaa gatgctccat tattattaac ttcaaaagat aagttagatt
    13801 catcagtaaa atctgaaata aagagagtaa tgggattaga tgataaaact ggtataacat
    13861 ctaaaaagac tgtttatata gctggtgggg aaaattctgt atctaaagaa gtagcaaatg
    13921 aattaaaaga tatgggatta aaagttgaaa gattatctgg tgatgataga tatgctacat
    13981 ctttagaaat tgctgatgaa ataggtttaa accataataa agtatttgta gttggtggta
    14041 ctggattagc agatgcaatg agtatagcat cagttgcttc taataaagaa atgccaatag
    14101 tagtagttga tggaaaagga aaagatttat caactgatgc aaaagacttt ataggaagtg
    14161 cttatgtaga tataataggt ggaaaatcta gtgtttctga agacatggaa gatgctatag
    14221 atgatgctac aggtaagagt ccagaaagag ttagtggaga tgatagacaa gatactaatg
    14281 cagaagttat aaaaacttat tttgaaaaag ataatagtga cagtgtaatt agcacaggtg
    14341 ttaaaaactt ctatgttgct aaagatggtt ctacaaaaga agatcaatta gtagatgcgt
    14401 tagcaatagc agcagtagca ggtcacaatg aagctccaat agttttagca actgatagtt
    14461 tatcttctga ccaatcagta gcaataagta aagttacaaa ttctgatgat tctaagaaat
    14521 tgactcaagt tggtaaagga atagcagatt cagttattaa gagaataaaa gatttattag
    14581 aattataaga taaaattaaa ctataaataa gagggaactt ctttgaagaa gttctctttt
    14641 ttataaaaag aaattaaaaa aggaactaaa aatagttcct ttttttgtga gaaataccta
    14701 aataattaaa gatgtaattt atttaagtat gattattata ctataaataa aaataattgt
    14761 ctactattct ataattttaa ggtataatag taattgtatg ataaaaaagg aggtaaatta
    14821 aatatgtcag ttttggatac aatattggat aaagcagatg agcaggaaat aagaaggctt
    14881 aatgtgatag ttgataaaat agaatcttta gaaaaagata tagaaaatat gtctgatgac
    14941 gaattaaaaa aaatgacaga cctatttaga gatagattaa aaaaaggaga aactttagat
    15001 agtttattac cagaagcatt tgcagtggca agagaagctt caaaaagagt tttaggaatg
    15061 cgtcaatata gggttcaatt aattggaggc attgtaattc atcaaggaaa aatagcagaa
    15121 atgaaaactg gtgaaggtaa aactttggta gaagttgcac ctgtttatct aaatgcttta
    15181 acaggtaaag gtgtgcatgt tgttacagta aatgactatc tagcacaacg tgataaagag
    15241 ctaatgcaac cagtgtatga gtttttaggt atgtctgtag gagtaattat tgcagatctt
    15301 atacctgaag ttagaaaagt tcaatacaat tgtgatatta cttatggtac aaataatgaa
    15361 tttggatttg attatttaaa agataatatg gtaactaaaa agtttgataa agtacaaaga
    15421 gagttgaatt ttgccatagt tgatgaggtt gactcaattc ttatagatga agctagaact
    15481 ccacttataa ttgctggaga gggtgatgaa gattcaaaac tttatgaatt agcaaattca
    15541 tttataaaaa caataaaaga agaagattat gaattagata gaaaagataa gaccgtagct
    15601 ctaaaggaaa gtggtattga taaggcagaa tcattttttg ggattaaaaa tcttacagat
    15661 atagaaaata tagaattata tcatcatata aatcaggctc ttaggggtca caaacttatg
    15721 gaaaaagatg ttgattatgt tgttgttaat caagaagtaa tgatagttga tgaatttaca
    15781 ggaagagtaa tggatggaag aagatataca gatggcttac accaagctat agaagcaaaa
    15841 gaaggggtag aaataaaaga tgaatctaaa actatggcta caattacatt ccagaatttc
    15901 tttagattat ataataaact ttctggtatg acagggactg ctaaaactga agaaggagaa
    15961 tttgaatcaa tttataaatt aaatgttgta caaattccta ctaacaaacc aattataaga
    16021 gcagatcttc atgataaagt gtttagaaca gagaaagaaa agtatcaagc tgttgtagaa
    16081 gaagtaataa ggatacataa aacaaaacag ccaattctta ttggtactgt ttctgttgag
    16141 aaatcagaaa tgttatctgg tatgcttaag aaagaaggaa taaaacatca agttctaaat
    16201 gctaaggaaa atgataaaga agcattgata gtgtcaaagg ctggtaagtt gggttcagta
    16261 actattgcaa ctaatatggc aggtagagga acagatattg ctttaggagc aggtgataaa
    16321 gaagaagaaa gtagagtaaa ggaattagga ggactttatg taataggaac tgagagacat
    16381 gaatctagaa gaatcgataa tcagcttaga gggcgttctg gacgtcaagg tgatccagga
    16441 acatcaaggt tttttgttag tcttgaagat gatgtaatca aattgtatgg tggaaaaagt
    16501 atagagaaac taatgaaacg accaaattca aataaaaata attctattga aagtaaatct
    16561 cttacaaagg caatagagag agctcaaaaa ggggtggaag gtaaaaattt tgaattaagg
    16621 aaaaatgttc ttaaatatga tgatactata aatgaacaaa gaaaagtaat ctataacgaa
    16681 agaaataaag ttctaaatga tgaagatata caagtagata ttcaaaacat ggttaaagat
    16741 ataataaaag aagctgctgc tgaatattta acaaaaagaa agagggatta ctatggatat
    16801 tttaagcatc tttataatac atttatgcca gctgatacct tactaatacc tgacttagat
    16861 aataaaagta ttgaggatat aattgatagt acttatgaaa tatcaaaaag agtttatgat
    16921 ttaaaaaaga tggtagtagg ccttgacaaa ataaccgaaa tggaaaaaat tgttctttta
    16981 aaagtagttg atcaatattg ggtagaacat atagatgcta tggagcaatt aaaacaatat
    17041 gtaggattta aatcatatgc tcaaaaagat cctttcaaag aatatgctat ggaaggttat
    17101 gagatgtttg aatcattaaa taaaaatata agagagacga ctatacagta tttatataaa
    17161 tttaattaag aaatttaaac tgtataggaa aaagaggctg ataatcagcc tctttttcta
    17221 tgtgagaaat acggaaaaaa gagatgcttg aaatttcttt cttccattta atattatact
    17281 ataaagaaga tgaaaagtct attagttata aactggatta ttaaaaaaaa ttacaatttt
    17341 gttacttttt tgtatacgta aatattaaaa aaataaaata taagaaaaag tttatatctt
//
LOCUS       DAYATN010000074        17361 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.074, whole genome shotgun sequence.
ACCESSION   DAYATN010000074 DAYATN010000000
VERSION     DAYATN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.074"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     misc_binding    <1..216
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            324..1805
                     /gene="thrC"
                     /locus_tag="ACGI8A_002318"
     CDS             324..1805
                     /gene="thrC"
                     /locus_tag="ACGI8A_002318"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFH0180314.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            1815..2708
                     /gene="thrB"
                     /locus_tag="ACGI8A_002319"
     CDS             1815..2708
                     /gene="thrB"
                     /locus_tag="ACGI8A_002319"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFH0180315.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            2838..3893
                     /locus_tag="ACGI8A_002320"
     CDS             2838..3893
                     /locus_tag="ACGI8A_002320"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFH0180316.1"
                     /translation="MKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVISG
                     VQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEAVK
                     AGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIVVE
                     GPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSSSD
                     VKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAIRN
                     KFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAYKI
                     DKLSTVEDVINELISEL"
     gene            complement(4048..4560)
                     /locus_tag="ACGI8A_002321"
     CDS             complement(4048..4560)
                     /locus_tag="ACGI8A_002321"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFH0180317.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            5080..6015
                     /locus_tag="ACGI8A_002322"
     CDS             5080..6015
                     /locus_tag="ACGI8A_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFH0180318.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(6107..7978)
                     /locus_tag="ACGI8A_002323"
     CDS             complement(6107..7978)
                     /locus_tag="ACGI8A_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFH0180319.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            8168..9094
                     /locus_tag="ACGI8A_002324"
     CDS             8168..9094
                     /locus_tag="ACGI8A_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFH0180320.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9233..9379
                     /locus_tag="ACGI8A_002325"
     CDS             9233..9379
                     /locus_tag="ACGI8A_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180321.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(9484..10008)
                     /locus_tag="ACGI8A_002326"
     CDS             complement(9484..10008)
                     /locus_tag="ACGI8A_002326"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0180322.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(10061..10285)
                     /locus_tag="ACGI8A_002327"
     CDS             complement(10061..10285)
                     /locus_tag="ACGI8A_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180323.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            10482..11606
                     /locus_tag="ACGI8A_002328"
     CDS             10482..11606
                     /locus_tag="ACGI8A_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HFH0180324.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISTFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSDSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(12080..12904)
                     /locus_tag="ACGI8A_002329"
     CDS             complement(12080..12904)
                     /locus_tag="ACGI8A_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5780 domain-containing protein"
                     /protein_id="HFH0180325.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(13252..14307)
                     /gene="ispG"
                     /locus_tag="ACGI8A_002330"
                     /gene_synonym="gcpE"
     CDS             complement(13252..14307)
                     /gene="ispG"
                     /locus_tag="ACGI8A_002330"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFH0180326.1"
                     /translation="MSYERRKTREVSVGAVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(14490..15494)
                     /gene="rseP"
                     /locus_tag="ACGI8A_002331"
     CDS             complement(14490..15494)
                     /gene="rseP"
                     /locus_tag="ACGI8A_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFH0180327.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(15507..16661)
                     /locus_tag="ACGI8A_002332"
     CDS             complement(15507..16661)
                     /locus_tag="ACGI8A_002332"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFH0180328.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(16825..17028)
                     /locus_tag="ACGI8A_002333"
     CDS             complement(16825..17028)
                     /locus_tag="ACGI8A_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0180329.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         5976 a         2701 c         2067 g         6617 t
ORIGIN      
        1 ggaagagtaa atctgaatcg attaccagag aaggaaacta ctagctgaaa tgtttcccta
       61 attagaagga ttgaaaacta cctaggagct tctatccgaa gttatcttaa tgattttagt
      121 aggaatagac gtttttatta cttgcgttaa agtatcaagt ggatttttta aaatcaattt
      181 gggtggaacc gcgggaaaca gattctcgtc ccttttttag ggatgagggt ctttttttat
      241 tacctaaatt cagtttttaa aatattctgt atctaattta attagctaat taaaaattta
      301 aaatatatgg aggattataa aaaatgtatt attcaagtac gagaggcacc gaagaaaaag
      361 taactgcatc acaagcaata ataaaaggaa tatcaaatga tggtggtctt tatgttccaa
      421 gtgaatttcc caatgtaaaa aatgaactta taaatttagt aaatttaacg tattctcaga
      481 tagcattttt tgtacttagt aaatttttat gtgattttac tgaagatgaa attaaaaatt
      541 gtatagaaaa tgcatatgat gaaaagtttg attgtagtag tatagctcca ttaaataaag
      601 taaatgatac ttatttctta gaactatatc acggtccaac tttagcattt aaagatatgg
      661 cacttactat aatgcctcat cttcttaaaa catctataaa gaaagataat ttagagaaag
      721 atgttgtgat tcttactgct acatctggag atacaggtaa agctgcactt gagggattta
      781 aagatataga taaaataaag ataattgtat tcttcccaga agatggtgta agcccagtac
      841 aaaaacttca aatgaaaact caaactggaa aaaatacata tgtagttggt ataaaaggaa
      901 attttgatga tgcacaatca ggtgtaaaga agattttttc tgataaagaa tttaatcaaa
      961 aactattgga taataattat atattatctt ctgcaaattc aattaatata ggaagacttc
     1021 ttccacaagt tgcatactat ttttattcat atatgaaatt agttagcaat agtgatataa
     1081 atttaaatga taaaataaat tttgttgtcc caactggtaa ttttggtaat attctagctg
     1141 gatattatgc taaacaaatg ggtcttccaa taaacaaatt aatatgtgca tcaaatgata
     1201 ataatgttct ttatgatttc tttaaaactg gtgtatatga taaaaataga acattgaaat
     1261 taactacttc tccttctatg gatatattaa tttcaagtaa tttagaacga ttactgtttg
     1321 aaatatcaaa tagaaatact gatattgtaa ataagctttt atcagacctt agtgataaag
     1381 gagtctacaa aataagtgat gatatggaaa aaaatttagc ctcattctat ggagaatatt
     1441 caaatgaaga agaagttaaa agcacgataa acaatgtgtt taaaaattat aattatttga
     1501 tagatactca tactgctgtt gcatacaatt gttatgaaaa atataaaaaa gaaacttctg
     1561 ataacacaaa aactgttatt gtaagtactg caagtccatt caagttttct gaagatgttc
     1621 taaagtctat agattgtgat tttaaagacc ttgatgactt tgctataatt gatagattat
     1681 caagtatttc taaaatagat attccaaaac ctattaaaaa tcttaaaaat gcagaaatac
     1741 tacataaaga tatttatgaa aaagatgaat taaaactagc tataaaaaaa ttcttaaagg
     1801 tataggtgat tattatgtta gagattatag ttcctgcaac aagtgctaat ataggtccag
     1861 gatttgattg tcttggaatt gcgttaaata tatacaataa attttatgtt gaagaaattg
     1921 aaagtggtct tgaaatagaa ggttgtgaag atgcttacaa aaacgaaaac aacttagttt
     1981 atacatctat gaagtacttt tttgatagag ttaaaccaga aaaaatacct gctggtatca
     2041 aaataaaaat ccaaagtgaa gtacctatat gtagaggtct tggcagtagt gcctcttgta
     2101 tagtagctgg agttatagct gcaaatgctc tatcaggtgc taatttagat aaaaatcaat
     2161 tactaaatat agcttcagaa atagaaggtc acccagataa tgttgctcct gctattttag
     2221 gaaatatgat tgtatctgtt actgataatg agaatataca ttatgatatt ataaaaattc
     2281 ctgaagaact taaattttgt gcaatgatac ctaattttaa gttatcaaca gaaaaagcta
     2341 gaggtgtttt accaaaggaa ataccatatt ctgatggggt attcaatgtt agtagagttg
     2401 ctttactaat atctgcctta ctaaacaaaa actttgacct acttaaagta gcttgtcaag
     2461 acaaattgca tcaagattat agaggaactc taattgagaa ctacaatgat attgttgaaa
     2521 aatctgaaca attaaatagt attggagtat ttttaagtgg agctggacct actataatgt
     2581 ctttaataaa agaaaatgat gatagttttg tggataatat gaaaaattac ctacaaaaac
     2641 taaaatctga ttgggaaata aaagagttat gttgcgattc taatggagct gttttaaata
     2701 taatataatt atcgtcttct atacaaaata taactactct tttattataa tttgtagtat
     2761 aataattagg actaggtaat aatattaagt attataatct agttctttaa acatgtttac
     2821 attgaggagg gattatcata aaagagttaa taattagaga tttagttgca aaagttccta
     2881 taattcaagg tggaatgggc gttggtatat ctaggtcatc actagctggt gcagttgcta
     2941 agcttggtgg tgttggtgta atttctggag ttcaaattgg atatgatgag gaagattttg
     3001 aaactaatac tataaatgca aatttaagag ctataaaaaa acatatttct aaggccaaag
     3061 aaatctcaaa tggtggtatt attggtatta attttatggt agctatgaaa gaatatgaaa
     3121 catacgtaaa ggaagctgtt aaagctggtg tagaccttat aatatctgga gctggattac
     3181 ctaataagct accatcatta gtaaagggaa gtaatgtaaa aatagcaccc atagtttcaa
     3241 ctgcaaaagc tgctaatgta attttaaaaa tgtgggatag aaaagagaaa actactgctg
     3301 acttaatagt tgttgaaggt ccaaaagctg gtggtcacct tggatactct aacgaggaac
     3361 ttgataacat agattctata gactatgata aggaatttgt tgaaatttta aaggttgcaa
     3421 atacttatgg agaaaaattt ggtagaaaca taccagtagt tgctgctggt ggtataactt
     3481 ctagtagtga tgttaaaaaa tatatagata tgggtgcaag tggtgtacaa gttggtacaa
     3541 gatttgttgc tacttacgaa tgtgatgccc atgagaactt taaaatggca tacataaatg
     3601 ctagtgaaga agatgttcat atagttaaaa gtcctgtagg tcttcctgga cgcgccataa
     3661 gaaacaaatt tatagaagaa gttaaaataa atcgtccaga aataaagaag tgctataatt
     3721 gtttgattcc ttgtaatcca aaagaaactc cttactgtat ttctcaagct ctaatcaatg
     3781 cagttaaagg agatgttgaa aattcacttt tattttgtgg aaatgatgcc tataaaattg
     3841 ataaattaag tactgttgaa gatgttatca atgaattgat ttctgaatta taatttaaag
     3901 aaaactaaat taaaatttta tcaagctaag gagtttaaat acgttattta ttaatcttta
     3961 taaacatatt tatgtcctta gcttttttgt gtcaatttcc ttttatggaa attttaattt
     4021 aattagagat agtattaacc tttataacta atcttctttt acaagtacat tggttatttc
     4081 agctatatat aaagtatgat aatctttttg tggataccat cttccatcta taccttcagc
     4141 aataaaacat tgaggttcaa gtttctgact atacattttt ttgcatataa tagccatttt
     4201 agattcttta aaataaggtg tatcatttaa gtgttctata ttaaatccaa tctgtgatat
     4261 cttatcctct tctctaccag atacctttcc acaatatgaa agctctttac gaaaatcttc
     4321 atcaaaaaat gtaagagaaa atttatctgt attatctata aattccttag tataacgctg
     4381 tggtcttatt actacaaaag caacatcttt tccccacata actccaagcc ctccccagct
     4441 agcagtcatt gtattcactt taccttcatt ttctgctgtt ataagtgtcc aatctttacc
     4501 tatcaatcca aatggattat ttttaatctc ttctgccaaa atctctttaa aatcactcat
     4561 atctctaata acctctcatt ctcttatatt tatattcctt ttattttcgt gataaattat
     4621 aaatgaagtc tagttttgaa aataaaatac ttttttaaat atattattgc aaataagtaa
     4681 atatatatat actctcttat tttttaatat atttaataat ttcactcaga atatatatat
     4741 tctttactca aattaaatat aacttatata ctttatgtgt aaattatggt aaaaatatta
     4801 tttaatcaaa tcaaaacttg atttatatac ctggaaaagg tattatatta aagagtgctt
     4861 aaaatacgca tttcataaat agctatatat aaaataataa ataaggagag tgatgggcaa
     4921 tggatactag tccgttgcgg agagaatttt ttacattttc atcacttaat aatatctaaa
     4981 atcaaaacta atacatatat tcagttatat aaattagtta attaatagat actattaaat
     5041 gatgagattt atataaaact gaataaagga tgtgttgcca tgataagata ttacaaaact
     5101 atagattcaa aattagagaa acttagtttt tttgaagatg gctgttggat aaatcttgtt
     5161 gaacctaatc atagtgaaat caacgaaatt tctaatttat taaatataga tgtagaaagc
     5221 atagaatctg cactagatga agaagaacgc tcaagaatag atgttgaaga caatcatact
     5281 cttatactta tagatatacc agtagacgaa agtgactcta attcttctca ttatactaca
     5341 atacccttag gtattatact aactgaagaa gctattgtaa cagtttgtga tgcacaaact
     5401 aagattttaa atgactttat agttggtcat attaaagatt tttttacgtt taaaaagact
     5461 cgctttttgc ttcaaattct tcacaaaaat gcagcttatt acttgcatta cttaagaaaa
     5521 ataaataaaa tgacaatcat aatagagcga gaaatttaca aatcaatgaa aaacaaagaa
     5581 ttggttcaat tattggaatt agaaaaatct ttggtatact tctctacctc actaaaatct
     5641 aatgaacttg ttttaaacaa aatggttaga actgctggta taaagaagta tccagatgat
     5701 gaagacctat tagaagatgt tatagttgaa aatagacagg ctcttgatat ggctaaaata
     5761 tatggagata tattgagtag aattatggat gctttcagtg caattataag taataatcag
     5821 aataatgtta tgcaaatttt aacagtagtt acactaattt tctcaatacc tacaataata
     5881 tcaggattct ttggtatgaa tgtcatcaac atgccatttt ctaataaccc aaacggattt
     5941 tggataatac ttcttattac ctctataata tgtatagtga ttacattttt catgtcaaga
     6001 aataagctat tataaaatat taataaaaag gccattagaa ttaattgatt tacatttatc
     6061 ctaatggctt ttatatattt aaatctatat aatcttataa tactttttaa ctatcatgtt
     6121 cttcatctat aatcatttca gtttcaagcc cttctagcat tgaatctaaa ctttctttta
     6181 tatttagtat ataaattggt acatactctt cttcatctat tacctcttct tgcaacttaa
     6241 atggaggaat taatacttca tcaaagtcta tatttaaatt tggaaagcct tctactatct
     6301 cttcacaatt atcgtactct tcatttgcta aagaaatgta catatctagg ctctttttat
     6361 aataaagacc tgcaagcata tactcacact tcttacttcc atatttaata ccttctttat
     6421 aatatttctc agcttctgcg tcatcattta attttaaata aatttgacct aaattatata
     6481 ttgattcaac acaaccttgt gctctagcct tttcatacca aaatttagaa tttacataat
     6541 ctttatagaa catatcataa attaatccta ccatatgttg agcatacaca ttattttcat
     6601 tggcaggtgt atcaaacatc attttagact cttccaattt accttctcta aaataaattc
     6661 tacctagatg tatacgagca ttcatatggt ttttgctact tgccatttca aaatactttc
     6721 tagcattttt tgtatttcct aagctctcat atatacaccc caatctataa ctagacctcg
     6781 tatctccatt ttcagatgct ttcttatacc aacttattgc ttcttcaata ttatttaaat
     6841 cttcatagat atttcctaat ttgatttgac aagatacatt atttggaatc ctcgaataat
     6901 acgaaatcgc tttttctata tcttgattct gataatataa atcccctaga ttttcaaggg
     6961 aacttttgca tccaacagct atagcatcct cataatattt aatagcattt tcataatctt
     7021 tttcttcaaa atatatacct gccagattat tttttgcttc tgttatatca tcttcagcag
     7081 ccattttata ataagttttt gccttctcta tctcattttc tctattatat aaattagcaa
     7141 gtctatactt agaaagtgta tgattttttt ctattgcttt ctcataataa taaattgcat
     7201 ctcccagctc ttcttttcta tcatatacat atgcaagcct atactcagac ttcacatcat
     7261 ctctatcagc actcttttta taatacttct ctgcatcttc taaattatca tttatttcca
     7321 taatcatacc caagttaaag caacctttca tatgacctga cttgtacaac tgtttatata
     7381 aacttactgt tgatttatta tctttctttt ttccatttaa tattgctaaa ttatattttg
     7441 catcatcatc taaaagcaca gaaccttttt tatagtattc atatgcttca tcatctttct
     7501 ccaaaaaagt atataatacc cctaaattaa aacaagcttt acctaaattc ttgtctgctg
     7561 ctgtttttaa ccatttttca caccttacaa aatctttcat tttaaaatgg catacaccta
     7621 aattattttg tgcttgtaaa taacctaaat ttgctatctt ttcataccaa tatatagcat
     7681 attcatagcc atctaactta tagtacatat tacctaaatc aaaagcagca tcttcacttc
     7741 cacttttata tgccatcgaa taccactttt ctgcttcttt aaattgtttt ttactttcat
     7801 atatatttcc taaagtatat tgagatttta cgtaattatc caatgcagca ctcttataca
     7861 actctattgc gctttccatc ttacccatct tttcaaggag ggcaccctta gtatgtacga
     7921 ctttcgggtc attagatata ttttttattt tttttataga aaattttgta tttttcatta
     7981 ttcgctacct acccatttta taaaatatat tctctcaaac attattatac attataatct
     8041 acaattaatg ctataatatt gaatttttac cttttgtcac tatattgtaa cattaaattt
     8101 taaattttat aatcattaga ttttattatg taattggtat ataatgtata tatagggggg
     8161 acattttatg aatttcagca atatttttat ccaagttgca gttttattta taattatttt
     8221 agttggatat tttgtcagaa aattcaactt attagatgac cactgcacat ctaagctttc
     8281 aactttgaca atgacagttt ttttaccatc aatgataatt agctccatgc aaattaactt
     8341 tgatagtaaa atgatacaaa aaatactatt gttattattt acatctttga ttatgtatat
     8401 agtttcaatt ataattgcat ttttattgaa atacatttta aaatgtaatg ataaaaaaga
     8461 tttgggaata taccagtata tagttgtttt ttcaaatgtt gcttttatgg gttatcctgt
     8521 aattgaagca gttttaggtc atgaagcaat attctatact gcaatattca atttgccttt
     8581 taacttattt tctttcactt taggaattta tcttttatct aaaggcagta caagtagagg
     8641 tttttcaata aaatcactta taagccctgc tacaatagct attgttattg gacttttttt
     8701 atttgtaaca ggacttaggt taccccaatt tataaacgag ccacttgaga tgttgggaag
     8761 tataactaca ccaatatcca tgattatcat tggaagctta cttgctaatt cttcagctct
     8821 cgattgtttt gtcaacaaaa aattatatat agtcactttt ataagattat tggtacttcc
     8881 tgtgattgtg tattttattc taaaaggatg gataaatgat aagatgattt tagctatacc
     8941 agtagtaatt tcttcaatgc ctgctgctgc aaatactgct ataatggcta atcaatatga
     9001 ctccaatata acactagctt cacaatgtgt gttttttaca acactatttt cagttatatc
     9061 tatacctttt ataagtattt tactcttatc ttgatttatt tttttatgta ttatataata
     9121 acatattcaa taaattacaa atataaacat tttattgtag tatattgatg gaattttttc
     9181 ctagatattt gtcaataata tctatatgta ttatagaata ggaggcttaa atatggatat
     9241 ggatttactc tttaaaatag tatttgctat agttgctata ggtgtttctg taaaaatcat
     9301 taaaactgtt gctagtttag cttttaaaat agcgttgata ttgctaataa ttttatttgt
     9361 atatagaatg tttttgtaaa catttaaaaa taagtttttt aacacaatag agaagtaaaa
     9421 tagattatct ttaatgtaaa ttttttatta aagacactct attttacttc tctattagct
     9481 tctttaaatt aaatttttat tatatcttcc cataatataa acatcttcaa attttccatc
     9541 tctgattaca gcatattttt tagttccctc tatttcaaaa cctagctttt tatataaatt
     9601 taatcccttt tcattatcca caattacttc taatcctact ctagttaaca taatccaatt
     9661 atctgctata tctaaaagtt gttccataag cttcttacct actcctcttc cctgataatt
     9721 ctctgcaaca cttattccta tttctgcgct atgtctcaat cgtggagaag aatttacatg
     9781 taacccagca aaacctatta catctttttc tccattatta ttagtttcag ctacaaacat
     9841 ataatcattt tctcccaaag aagctaacat agcttcagtt ttatcgactc tgtcactcat
     9901 taatgccaaa gtatttctca taacactgta tgttcttcta atttcattaa aagcttgtgc
     9961 atcttctctt cttataggtc ttattgttat atctataggc ttactcatat tggattcccc
    10021 ctcaaacata atctttattt ttaattaaaa ttttacatct ttattttata ctgttcaaat
    10081 ctatgtatgc ttctacctga ggtacatata tcatattatc ttttatatgt gtacttccaa
    10141 attcttttaa ttctgtacct tctttatcct tagttatttt atatctaatg gattccatac
    10201 cttcaactac ttcagcatga aaaaacatac cttcatattg tttaaatcta aaattaaaat
    10261 tgtctttagt aagttttatt atcatatttt tatctactcc ttatataatt gatatttttc
    10321 ttattattag ttgtatatat actgcttatt catattaata tattataact tatcatttat
    10381 ttagtataaa gttataaata gatatattta tacttatttt acaatctttc ttcaattgat
    10441 atataatatg tttatataaa ctaatgtatg tgaggtgaaa tatgagcaaa aaaacaaaat
    10501 atttttcttt aattctcttt cctattgctt tgcttttaaa ttttatagcc agtaaagttc
    10561 caaatattgt tgaaaaatat tactcccaat tcatagataa aataattgta caaatattga
    10621 gtaaagtttc tggaattttc ccattttctc tctatgagat tacaatgtac ataatagtta
    10681 tatctacctt cttattttta tgttgtactt tatctacaat ttttaataag aaaaaaaaat
    10741 taaacatata tttaaaaaat tccattttaa atatattatc cattgtatca atagtctact
    10801 ttctttttgt agtgctttgg ggaataaact ataataggat tccactagaa accacactta
    10861 taaataacta taatttaaaa aacaatacct ctatccaatc aaaacaatat agtgttaaag
    10921 agcttactaa attatataaa ttccttgtaa ttaaagcaaa tgaaactaga aaactcacat
    10981 tacaagataa aaatggtatt gtaaaatcta atacagatta taatggtgtc ataaatagag
    11041 ctcaactagg atacgataat atcttggata tattacctgg tgttagtgat agttattcta
    11101 agcctaagta tattatttca tcaaatctaa tgtgctatac aggaataact ggtatatact
    11161 ttccttttac aggtgaggca aatgtaaata tagcaatacc tgacttatat attccttgta
    11221 cagttgaaca tgaaatggca catcaaaggg gatttgctag tgaagatgaa gctaatttta
    11281 tagcgtatct tacttctata aaacacccca atattgattt taactattct ggatatattt
    11341 tagctttaaa ttatactgct tctgctttaa gtaaggttga ttataatgca tatgttgata
    11401 tttctgctgg catttcagat tctgtccgta gagatttaaa aaatgaaagt gaattttggc
    11461 aaaaatatga aggtaaaatt aatgaaattt ctaatgagtt taacaattct tacttaaaag
    11521 ctaatggcgt tactgaaggt actcagagtt atggtaaaat ggttgattta ttgctaactt
    11581 attatgaatt atatccatat aactaaacac tacaattcaa aattttattc atatacagct
    11641 gatattaaat cagctgtttt ctatttaaat aaattattta acttcaagca atcttatata
    11701 atttgatagt acttatacta tctgtataac atttcaattc taactatttt agttaaaaca
    11761 tttctattat cttttttaat aaatgtttta actaaaattt ttaaaacata aaacttccac
    11821 attatttcac tatctatata aaaatctaaa aattcacata taaaattaca taactaaaaa
    11881 atcataaatt tatagaaaac atatattcaa aataaaaaag catgtatcaa aacttgacac
    11941 atgcttataa tcttatgtat atatatcatt ttgtcacaat aaaaatttac ttgtaacact
    12001 gatattttta aacaaaatag tccttattta attaaattaa cattaattaa attcgtactc
    12061 attttgctat cattaaacat tacttatagt aattttctaa tacactattt gaaacttcct
    12121 tcccttgaaa acttttcaac caattctcat atgctgagtt tttccataca ctaccatcat
    12181 taaatgttac ttgttttaca caagatataa tgtactttac accatgctct ccagttgatt
    12241 gtttccattt atcaaacaat agaaaggcat cattttgatt cttacctggt gctattgcat
    12301 tttgctttgc atattctttc tccatttgtt tccattcttt caaccaattt tctacccatg
    12361 ccttgccttg ttgtttttca aacatactta ttatatcctg tggtggtgat tgttgcattt
    12421 ttctataagt atgtgaatat gattttggag acacaggaga tatgcctgta acaataccat
    12481 agtctacacc agctgggcta aatccaacac ttcctacttc gccatctgct gccacatttt
    12541 gagcatccca atatagctca agtggttttc catttttatc atatgctaag catgtaattt
    12601 cataatccat aattgccttt tcactgctat ttgttaagct tataacattg ctaggccaat
    12661 gatatggagt atcatcatac tgtattttgc tattatttat aaataatgga atattctcat
    12721 tataaacaag acctttacta ctttgaatat ctgatacctt ctttgtgaca gtacttaccg
    12781 tatttgctaa gtatacattt gttttttctg aattggtcgt actaagagaa aatgttgttg
    12841 tcgttccagt aatcagcaaa gcaacaacta aaataggtaa gctagatatt tttttatatc
    12901 tcacaattct tcacctcttt ttctattaac ttataataaa acaatttacc aaaacattaa
    12961 aatctatgtt aacataattt accaactgtt ctagtaacta tatcctatca tagttactat
    13021 caagtttcaa tactattgat ggaaattttc actatattaa atcttttttt aattttataa
    13081 atttacaata tgatttaact catacatata tagtcaaatt agaatataag tttcttacat
    13141 ttaatgaagt ttataaaata aaaaatgact agataattaa atactgtaaa taaaaactta
    13201 ttacaaaagt atttaaaaat ctagtcattt aatatatata tgaattgtat actataattt
    13261 ttcaatctct tctagcaact catcaactaa tttgttacca tcaatcattc taactatttc
    13321 acccttttta aataaaagac cttcaccttt acccccagca atacctatat ctgcttctct
    13381 ggcttcacca ggtccattta cagcacagcc cataattgca acagtgatat ttttttccat
    13441 actaccaatc ttttcttcaa cctcatttac aacactaatt aagtctatat tacatctacc
    13501 acatgtaggg catgatataa ctttaatctt atcatttaaa agtcccaaac ttcttaaaat
    13561 ctgtttacca acaataacct cttcaacagg gtcgcctgtt aaagatattc taacagtatc
    13621 tccaattcct ttaagaagaa gtgctcctac ccctatagaa gactttattg ttcctttgtg
    13681 aacacttcca gattctgtaa ttccaatatg aagaggataa tcaacttttt ttgatattaa
    13741 ttcatatgca tcaacagttt tataaatatc ggatgatttt agagatataa ctatattatg
    13801 aaaatctaag tcttcaagaa tcttaatatg tcccattgca ctttcaacta atgcttcagc
    13861 agttgcagag ccatactttt ttaaaagttc tttctctaaa gaacctccat taactccaat
    13921 tctaattttt aaatttcttt ccttgcattt ctcaactacc atcttaactc tctctatact
    13981 tccaatatta ccaggattta ttcttattcc atccacacct tgttctatag cttcaagggc
    14041 tagcttataa tcgaaatgta tatctgcaat aattggtata tttacactag actttatctt
    14101 tgctatattt ttagcagctt taatatcagg aacagcaact ctaactatat cacaaccagc
    14161 ttcctctaat ctctttattt gagctattgt agcatctgca tctcttgtat cagtatttgt
    14221 catagattgt atacttatag gattttctcc tcctattttt acagctccta cacttacttc
    14281 tctcgtcttt cttctctcgt aactcatatt ccacctctat atattatttt atattgaatc
    14341 tctttaaata ttaaaatatt ataacctaca tgtatattat aaattaaaaa gctatctagt
    14401 taaataagaa taaatttcat atataaaaat taatttattc ttatttaaaa ctacatagcc
    14461 tattttctta aaatctctaa aaaactaaat cattgaaata atcttagtat atctttatat
    14521 gtgataaaaa gcataaataa cattaaagct ccaaaaccaa caacatttat catgccttct
    14581 ttatttgggt cgagtttttt accacctcta acagcttcta gtaacaacat aagtattctc
    14641 catccatcaa gtgctggtat tggtaataga tttactatac ctaaatttaa gcttatgaca
    14701 gctgctaaat acactacatt tatgatgcct gtacgagcag catctgatac cataccaata
    14761 acacctactg gtcctgctac agcattgcca atccctcctg gtacacgtcc tgtaatcatt
    14821 tgacctagaa atgttagcat ctgttttgtc atatctacag tagttttaca tgcatttact
    14881 attgacccta aaaagtcttt ttctctttgt ggttgaattc ctatttcata ctttccattg
    14941 ttatttttag gtgttacatt tacaacttta tcacttccat ctctagttat actaagtttt
    15001 aattctccac cagaagaagt gtttattata tttgcaactt catcccaagt cttaacagaa
    15061 ttaccattaa ttttatttat cttatctcct acttgaagcc ctacagcctg tgcaggagta
    15121 tctggcatta tctttcctaa tttagtcgtt ggagaaccta tatacaaaaa tactggaact
    15181 aaaagtattg ctgctaatat tatattgaat attggtcctg ctacaatagt actaaatctt
    15241 tgtaatatag acttgtttcc aaaagaattt ggacttattt gttcttcatc ttcaccttcc
    15301 atgctgacat atccaccaat tggaagtgct cttatagaat actctgtatc tttttttaca
    15361 ctatatatct taggccccat acctatagaa aactcattta ctttgatacc acttcttttt
    15421 gcaaatataa aatgtcctaa ctcatgtatt aatacaatta tactaaatag tattaatgcg
    15481 gctattatag tcataaattc accttcctat ttttttatca gatttgcaac ataagctcta
    15541 ctccatctat ctgtttctaa gatttcttct aatgttggtt cacttatgct actatgaact
    15601 tctaaagtct tctctatgta atatggtatg tcatagaaac caattttatc ctctaaaaat
    15661 tcacttacta aaacttcatt tgctgaattt aaaacagaag aataagttcc tcccattttt
    15721 aaacactcat atgccaattt taaacatgga aaagtttcta aatctggttc ttcaaaagtt
    15781 aaagtactaa attttgaaaa attcattctt tcaaagctac tttccattct atctggatat
    15841 gtaagagcat actgtattgg tagtctcata tctggacatc ctaattgagc aattattgag
    15901 ctatctgtat actgcaccat tgaatgaatt atgctttgtg ggtgaactac tacatctata
    15961 ttttcttgtt ctactccaaa taaccatcta gcttctatca cttcaagtcc tttattcata
    16021 agcgttgaag aatcaatact tatttttctt cccatactcc aatttggatg cttcaaagct
    16081 tcatttttag ttatatttgc aagttctccc tttttctttc ctctaaatgg accaccagat
    16141 gctgtaagta ttatcttttc tatatttttt ttattttctc catttagaca ttgaaatata
    16201 gcactatgtt cactatcaac aggaagaata tttacattgt atttttctgc ttctttcatt
    16261 acaagttcac cagcagttac caacgtttct ttattcgcta atgctatgtc tattcccttc
    16321 tttatagcac aaagagttgg gactaatcct atcataccta ctacagcagt taaaaggaca
    16381 tccacttcat caagtgatga tattattttt aatccttcca ttcctgctaa aacttctatt
    16441 tctatatttt taggcaacat ttcttttaat tttaatgctt tattttcctc aaaaactgtt
    16501 acatatttag gtttaaactc tactatctgt tctagcaata attcaatatt actgttagct
    16561 gatattgcaa ctatctcaaa cttatctcta tgttctctaa ccacatccaa agtttgttta
    16621 cctatagagc ctgtagaacc taaaattgat atctttttca tataataatc ctttctaata
    16681 ataaatatac cctgtcaaaa cattataaca ctactaaaac aattattttt actattcata
    16741 gaaatttcat atattattcc acagattaaa attaatctat tattttttgt attttgtgga
    16801 atattataaa atattctagt attattaaat gccctttttt atttccacaa catcttctat
    16861 tttacagtct agtgctaaac atatcttctc cattgtatcc atagaaacag atttatcatt
    16921 tcctagtctg cctaaaacag aaatacttat acctgatata tttacaaatt ccattttctt
    16981 tatttttctg tcaattaaca atttccataa tggagcgtat gaaatcatgt tatcacctcg
    17041 caaaatatat tctatcacat tttatctatt ttggaaaatt ttcatctatt tagataaata
    17101 atcacctgct taagtgtcaa ataattttat ccttctaata tgaaaattta atactatact
    17161 aaatgattgg tatataacac atatgatgca ttattattaa catataaaaa tttttattgg
    17221 tgctaaatta aagtaatata ctattattat atttgcagat atataagaaa gattttatta
    17281 tatatttaaa gtacttgatt ataataagag acaagggcta tgatggatac tatagaaagt
    17341 tagaactata ctattttaga a
//
LOCUS       DAYATN010000075        17312 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.075, whole genome shotgun sequence.
ACCESSION   DAYATN010000075 DAYATN010000000
VERSION     DAYATN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.075"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            88..834
                     /locus_tag="ACGI8A_002334"
     CDS             88..834
                     /locus_tag="ACGI8A_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFH0180330.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            895..1569
                     /locus_tag="ACGI8A_002335"
     CDS             895..1569
                     /locus_tag="ACGI8A_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0180331.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKAEELADWISEYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            1937..3283
                     /locus_tag="ACGI8A_002336"
     CDS             1937..3283
                     /locus_tag="ACGI8A_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HFH0180332.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNIIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEKAYSIFYGDILESCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3310..4710
                     /locus_tag="ACGI8A_002337"
     CDS             3310..4710
                     /locus_tag="ACGI8A_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFH0180333.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            4920..6356
                     /locus_tag="ACGI8A_002338"
     CDS             4920..6356
                     /locus_tag="ACGI8A_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HFH0180334.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            6700..7644
                     /locus_tag="ACGI8A_002339"
     CDS             6700..7644
                     /locus_tag="ACGI8A_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HFH0180335.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7813..8745
                     /locus_tag="ACGI8A_002340"
     CDS             7813..8745
                     /locus_tag="ACGI8A_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFH0180336.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8850..9317
                     /locus_tag="ACGI8A_002341"
     CDS             8850..9317
                     /locus_tag="ACGI8A_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HFH0180337.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9561..10433)
                     /locus_tag="ACGI8A_002342"
     CDS             complement(9561..10433)
                     /locus_tag="ACGI8A_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFH0180338.1"
                     /translation="MKGNSLMLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSIS
                     AFIFSTIITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQ
                     LTFTTLLAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVL
                     LSSLFLSLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLIT
                     GILWSIANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGI
                     ILIMSGVFMLSIIK"
     gene            10581..12545
                     /locus_tag="ACGI8A_002343"
     CDS             10581..12545
                     /locus_tag="ACGI8A_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0180339.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12545..13006
                     /locus_tag="ACGI8A_002344"
     CDS             12545..13006
                     /locus_tag="ACGI8A_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0180340.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            13009..13329
                     /locus_tag="ACGI8A_002345"
     CDS             13009..13329
                     /locus_tag="ACGI8A_002345"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFH0180341.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13345..14445
                     /locus_tag="ACGI8A_002346"
     CDS             13345..14445
                     /locus_tag="ACGI8A_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFH0180342.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14565..17165
                     /locus_tag="ACGI8A_002347"
     CDS             14565..17165
                     /locus_tag="ACGI8A_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFH0180343.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLNKSNDAFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTIFTKGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNKMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6883 a         1783 c         2998 g         5648 t
ORIGIN      
        1 aacaaaaaat aaaaatgttg attatattaa taaactttaa tataatatat ttatgaaaaa
       61 tgtaaactgt gaaatggagg tagtaacatg aaaattgaaa atctttctat tgatgatatt
      121 aaacgaggct atatatttaa tgatgattta aattgttata gttgcattaa atgtacaaaa
      181 acatatgagg taggagaaat atataaaata ggtaatagat tctttgaagc ttcaaaagct
      241 attgaacttc atatggaatt agaacatagt gattatttag agcaattgct taatagtgat
      301 tcaaaataca acacattgac tgaaaatcaa aaacaattat ttcaattatt tattagtgat
      361 ttaccagata aggagatagc aaaacaggtt ggagtatcca catctactat acgccatcaa
      421 aaatttattt tcagagaaaa agcaaaacag gctaagctct atttagcact atatgaatct
      481 gtatttgaag ataaatctaa taaaagtagt actataatac caatccatga acatgctcga
      541 atggtagatg aaagatatgt agtaactgaa gaagagagaa aacacatttt ggatacatca
      601 tttgaaagta tgaatccttt aaaattaaaa gtattttctt caaaagaaaa gaaaaaggtc
      661 gtaattttat ctaagatttt ggaacaattt gaacgtggga agaaatattc agaaaaggaa
      721 gttaatcaga ttttagaatc tatatatgat gattttgcaa caattagaag atatctaatt
      781 gagtatggtt ttatgagtag aaataaagaa tgtacaaaat attggttaac ataattaaaa
      841 taataaattt tactaatgtg taatgttatt tttatctagg gggaggagtc aaatatgaat
      901 gatgaattaa aatctatttt atacaataaa ggtgtggaca ttgttcgctt tgttgatata
      961 tctgaatttc caataaatca aacgcaaggt ttttctaaag caattttatt ttgtataggg
     1021 ttatcaaaaa aatttattac agatatatac aataacttac ctacagatag tgatgagttt
     1081 ttagaaaaag aggagaaagc agaagaatta gctgactgga tttcagaata tattcaaaat
     1141 aaaggatata gagcgtattc tcaatcggag aaaaacaatc tagaacatgg atattttgaa
     1201 aaagcctata ttaatccaga aatgcaatca gggattagtc ctttaccaca taagacaata
     1261 gcaaatatta gtggcattgg atttatggga aaaaataatt tgtttgtaac agaggaatac
     1321 ggatgtgctt ttagcatgtg tacagtactt acagatgctc caatttctgt tgaaagatat
     1381 cctttgattg attcaaaatg tatggattgt aatgtttgtg tagaaaactg ccctgcaaaa
     1441 gctatacatg gaaatgagtg gactttgcca ggaaaaagag agtctattat tgatgtttca
     1501 aagtgttttt gtgtattgaa gtgtatgatg agttgcccat ggtcattaag atatgcaaat
     1561 caaaaataat tttactagag aaggtattaa attgaatatt ataaaatttt taaaatgtaa
     1621 gattatataa gatttttcaa aataaaaatt tgtatagaac actcagaaat gagtgttttt
     1681 ttattggaat taaattatat tgatacaaag tttaactatt ttaatcatta tataaaattt
     1741 gtattaaaaa attaagaata acttggtaat agtgaaaata taatgagatt ataattgatt
     1801 tataatataa ctataatgat gttgtaaaat gaaaattgtc atttcaaaat tcccaaaaag
     1861 agtaaaagtc acttcggtta tagtcaaaaa attttttcag tgaaaagaat ttagatttaa
     1921 aagggaggaa ttaatcatgg aaattgaaac taaaactccg gattggatta atgtactaga
     1981 attaaacagt aaaatagata ttactgggaa atatgatgta agtaatatta tacaagatat
     2041 tgtctctgat aagtcactag aaggttccat tatatatttt ccaaaaggta attatttatt
     2101 tgagaaaggt attaaaatta gtcaacaaat aacacttcaa ggagattctt attatggagg
     2161 agataatcaa gtatcaaatt tagataaaaa acaatcaatc ataggaacaa ccaattttat
     2221 aactcgagga gtttctaata tgagtattat tgcattgaca ggaacaagtc aatgtatcaa
     2281 aaatattaat ttttattatg atagtcatga tatagagaaa ataccgccta aaaatgtatc
     2341 tgctattaca gaatatggag aaacacaagg actatctcat tttgaacacc tgtttatttc
     2401 tggattttca gggataggga ttgaaattcc atactattca actggcaatg atataattgt
     2461 tagttcatgt ggtcttggta tgaggcttgg agagaagtca atgctttcta gtagcaagat
     2521 ttacgaatgt aaaaatggaa tggagattac tactggtgtt agcttaaata atgtgagaat
     2581 agaagaaata aaagaaatag gtattaataa taaaggattt ggttttaacc tgattatgaa
     2641 tcttatggtc gaccaatgtg gatattgtgg atttatgttt gaaaaaatgt gctatagcca
     2701 gataactgcc agaattacta gatgcggtca gtattataaa agtgttgatt ataatgctta
     2761 tgagaatatg gaagatagaa aagaaaaagc ttattccatt ttttatggag atattttaga
     2821 aagttgcaat attgtattga tgaatagaaa tattgatacc ttagaaagtg attcacttaa
     2881 tgaacataag atatatgtaa tcaaggcaaa tgaaacaaac aatgtaatgc taacatgtta
     2941 tgctgaagca gataacttta tacaatgtgc aaagggcaac ttgctgttag aaaatggtga
     3001 aaatacttat aaattttata atggtgagat ttgttctgta ggaggtgttc aaatttctga
     3061 tatagatgaa aaagatttaa tcaaaataat agagaacact ttgtatatag atgataaagg
     3121 taagtattta atggttccta gattaaaaaa gaatactgta atatattcag tactagattc
     3181 tacagaagaa ataaataaat tacatggtgg aatatgggaa cagattgatg taaaagtatt
     3241 agctggggaa tccatgtact attataaaaa gataaaagat taaattatca aactaggagg
     3301 tagtgattaa tggaagagtc tataataatt aaagatataa gtggctggat taatgtactt
     3361 gattatggaa tttgtaatga tgaaacaaaa gatgtaagtt ttaagttgca agagattatg
     3421 gatgctgtac cagaagattc tattatatat tttccaaaag gaaaatactt atttgaaagt
     3481 ggtgttaaga ttaagaaacg attaacctta tgtggagatt cttatatttt agcgaatgtt
     3541 cctaaaaata ctgctggagt aacacagttt acttttaaag gaaagaaaaa tcctaaaagt
     3601 gaaaatagag caatcataac agcaaaaagt gtaagacact gtattaagaa catttgcttt
     3661 tattcagata gttgtgatag agatatgaca tctaatcatg aacctccaac agatggaaaa
     3721 cctagatttc atcataatgt aattataaat tatagaaata tatctgctgt tatatgtgaa
     3781 gataaacagg gagaaccagg tcattatgaa aatctatctt ttagtggttt ttctggaatt
     3841 gcacttgata tgcctaataa ttctacagca aatgatatta caatttttac atccggactt
     3901 ggaattagta caggggaaaa ttcaacaatt gctaacagta aggtttgggg ctgtgttgat
     3961 ggaatgataa tttctactgg aacttttata aatggtatga gggtagaaga aataaaaaaa
     4021 gttggaatta aaaatattgg tagaggttta aattttatta caaatatcac aattgaccaa
     4081 tgtggatatt gtggagtttg gtttgattct atatccaatg ttcagatatc aggaaatatc
     4141 acaaggtgtg ggcagtacta ttatgatgta agctatgata attatttaat gttggataat
     4201 agagttaaag aagcatattc tttattctat ggtaatttat tggagtcaag taatatagtt
     4261 ttatcaaata ccaatgaaga taattgggaa gatttcaaag aagataagca taaggtgtat
     4321 cttatagaag cacttaaaac caagaatgta ctattaacat gcaatataga tgctactgac
     4381 tggattgtta agtcagaaaa aggaaattta acgtacaata atatgcgaca aactgttata
     4441 ttttacgatg gaaaactaag tagtatcaat ggaattggta tatctgatga agattatggg
     4501 gataaagtca agataaaaaa aggaaatata tatataaata gtaatgataa ggatattaag
     4561 atgcataaac cagaggtagg tgacattatg tcaacaatta ttaaagataa aagtattctt
     4621 tctgctcatt atgggggaac atgggagaaa attggtgaaa aaatagagtt tggaataact
     4681 gtacactatt ataaattgat agagaaataa gtaaatacgt agtatttgaa tagatgtatt
     4741 ttattggaga gttaggacta tagttttaat ataaatggtt tggtaatagc atggattgaa
     4801 taatataaaa ataatataaa aaattaattt gattatgtaa atttctatga agtagtaaaa
     4861 ctgtgacttt agtcatagga ggttcaaact aagtaaaagg gaaaggaata tataatatta
     4921 tggaaggaag aactgggatt aaagatggaa gatggattaa tgtagtagaa agaggtatta
     4981 aaaatgatgg ttcgtacaac ataggtcctg ctttgcagga tattattgat gaaatagaaa
     5041 cagatttttg tgtcttatat tttccaaaag gtaaatacct atttacaagt ggtgttaaaa
     5101 ttaataaaga aattgtattg caaggtgatt ctaattcatc tacaggtact acacaattta
     5161 taactcgtgg agtagaaaat atgagtatta ttacattgac agggaagaaa caatgtatta
     5221 aaaatataaa tttctattct gatagttgtg agatacaggt aaataaagaa ccaccgacaa
     5281 aaggtaatcc taaatatcat tatgagatga ttattaataa gaatgaaaag aatgttgaga
     5341 tgaataatgt atcagcaatc ctgtatgata acagtaagaa tatgattaaa ggtctaggtc
     5401 attatgaaaa tatttatata agtggttttt cgggtacagg aattaggata ccatattact
     5461 caattgtaaa tgatattaca gtttcatctt gtgggctggg tattgataca ggaatagaca
     5521 caattatttc taatagtaga atatctaagt gtcaaaatgg aatgaggatt agaactggaa
     5581 ctagtataaa taatgtaagg attgaaaaaa tacagaaagt tggtcttata agtagtcaag
     5641 ttaaggggta tgaggggtat ggaagttata aaattaataa tattacgatt gaccaatgtg
     5701 ggtattgtgg cttttcattt gattcaatga aagatagtta tttttctggt attataagaa
     5761 ggtgtggtca gtattattat aatacagatt atgacacata tttaagtatt aaagatagag
     5821 tagaagaagc atattcttta ttttatggaa attattttac agaatgtaat attgatttag
     5881 ttagtgataa taaggataac tgggatgatg gcttagaata taaacataaa gtttatgtat
     5941 ttaaaaccct tcaaatacca aaattaacat tgagatgcaa tatagatgcc tatgattata
     6001 tcgttaaatc ggtacatgga ggaggtaatt taatgttaaa aaataacttt aacaattata
     6061 tatttattga ttcatctaat ccagaagtta ttaatggaat taattttaat tatcaaaaga
     6121 atggaaactt gttaaggtta aataatggta aattaagtta taaaaatgga aaggaagaaa
     6181 cagctatatt cagacctaat tatggtctta ttgtagcatc aacagattca gaatgtgaac
     6241 aaatagaggc tacctatgga gaaaaatggg agaagcttgg ttctgaagta attggagaag
     6301 aaacggtata ttattataaa catcatgaac cagataaaac aaaccaaata caataaaatt
     6361 tgtattatat aatgatataa agttttaata tatagtgttt acctttaatg taatattggt
     6421 atatttgtat agtattaaat ttgttaaaaa gtaaaattgt agggtgcttg aaaatagcac
     6481 cttttttagt ttcattataa aattagtttt gaaagtttag gaataatgta tatttatttt
     6541 taaagaatta taaccttatt aaggatattt taaatgctag aaatgaatca gtaaatcata
     6601 ctggaattat cttaaatgtt ttgattaaat taagtattta gagctataat tatattaaaa
     6661 atatattaga acataattat attactatgg aggttaaata tgatagtaag tgtgagtaga
     6721 agaactgata tacctgcttt ctatagtaag tggtttttta acagacttga agaaggtttt
     6781 gtttatgtaa ttaatccaat gaatccaaaa caggttagta agatagagtt aaatccacat
     6841 actgttgatt gttttgtatt ttggacaaag gatgtaactc caatgttata tgatttaaat
     6901 aaattaagag actataaata ttattttcac tatactataa catcatatgg aaaagaagta
     6961 gagacatgta ttttagataa aagaaaggta atagattcat ttaaggaatt gtctagaaaa
     7021 ataggcaaag aaagagtaat tttaagatac gacccgattt tcttaagtga aaagtacaca
     7081 gtagattatc atatcaaagc atttgcgagt ttatgtaatc agcttgatgg atttacagaa
     7141 aaatgtataa taagttttat cgatttgtat aagaaaacaa aatttaatac taaagcatta
     7201 catataaagc caatagaaat tacagaaata gaaacattat caaaagagat ttctaagatt
     7261 gctcataagc atgggataat acttgaaatg tgttctgaag aatataattt aagtaaattt
     7321 gggattggaa aaagtaagtg tattgatgat agattaatat caaaaattat aggatgtgaa
     7381 gtagaggtaa aaaaagatag tacacaaaga gatatttgtg gatgtgttaa aagtgtcgat
     7441 ataggacaat acaatacttg cagacattat tgtctgtatt gttatgctaa ctttaattat
     7501 gcaaaagtag aagaaaactg taggctttat aaggaagata ataaattact catagatact
     7561 ttaagagatg atgcaaaaat aagcattaga gatatgaaga ctattaaagt agataagaat
     7621 aaacaaatta gtatatttga ttaaaataaa tatactaatt accttaatat attaagaata
     7681 tagatacttt tgataacaaa attaaactta atagatattt tctatataag aaagatggtc
     7741 ataatatgtc aatttgacca tctttttgat ataattaaaa cacaaataat ttagtaacaa
     7801 aggaggcaga acttgaattt attaaaaaaa tatcctatac ctattgcaag tttgatttta
     7861 tcaattttta ctttaggtaa cttattacaa tcttatagtg aatcattacg taatgtaatt
     7921 ggatttatag gatttatttt gtatgcaatt tatatagtca aaataatatt acttagaaag
     7981 ggtgttaaag agcaattaga gaacccttta atatcaagta catttccaac tataacaatg
     8041 gcaacaatga tatttgctac atatataaaa ccattatctt tagaattaag tattatgatt
     8101 tggagcatag gaattgtggg tcatcttgtg ttaatagtct tatttagtaa gaagttctta
     8161 gttaaattct caataaaaac ggtatttcca tcttggtata tagtatatgt tgggatagtt
     8221 gcagctagtg ttacggcacc agcatttaat caacttttga ttggcaaaat tgccttttgg
     8281 ataggatttg tatcttatat agttcttata ccagtaattt taaagagagt atggataatc
     8341 aaggaaatgc ctgaaccatc cttaccaagt atagctatat tagctgcacc agggtcacta
     8401 ttattgtctg gatatattaa ttctttttca gataaaaata atgtagtatt atacttgtta
     8461 ttcatattat caattgtatt ttatattata gttttgggat atttaccgaa attattaagg
     8521 ttgccttttt atccgagttt tgctgcattt acattcccta tggcaataag tgcaatgggt
     8581 atgaaactaa tgaatggtta tttaactaaa tcaggtcatt ttataagttg gatttcatat
     8641 gcttctaagt tagaagagat aattgctaca gtgattattt tatatgtttt agttttgttt
     8701 attatatatc ttagtaaatc tgaaactaca tctaaagcta aataagaatt attaaatatg
     8761 tgtatttaat aggaatattg atataaatat atgaagataa gataaaccat agaaacttaa
     8821 tttagcttaa ttttattgga ggaaaacaaa tgaaaacaaa agaagaatat ttaaaagaag
     8881 ttagaaacat atcagaagaa tactttagaa aaggagagtt tttttgctct gaagcagttc
     8941 tccagacaat aaatgatgct ttaggccaac ctttaagtcc agagataact aagttagcat
     9001 caggatttcc gataggactt ggaaaggcac aatgtctttg tggagcaata tctgggggag
     9061 agatggcttt aggtatagta tatggaagag ttcatggtga agctatgaat ccaaagatgt
     9121 ttgagcatgc taaaggtttg catgattata ttaaaaaaga gtatggtgca acatgttgta
     9181 gagctattac aaagaaatgg gatggtgata actttatgtc tcccgaaaga aaagagcact
     9241 gtattaaaat aacaggtcaa gttacagagt gggttgcaga taagcttata gaagatgaaa
     9301 aacttaatat caaatagtgt aatattagtt ggtgaattta taaagttaaa tttacttata
     9361 gtataagata cttgatattc tcgtattgat agaaaaattt atagcatatg tatttaatga
     9421 aatatactta tatttagagt gtggtttaat ttcataaata catatgctat tattttaagt
     9481 atataagcaa atttatttaa ataaatagaa acagttaatt tatatgtaca aaactatata
     9541 tgattagtgt aaataaatac ctattttata atactcaaca taaagacacc agacatgatt
     9601 agtattatac caataaaaat agcaagccat tcttttttgt tcttcttttc tttaaaaaat
     9661 ataatccctc ctaaagtggc tactatcaca caactctgag agatagaaaa acttattgta
     9721 actcccaatg tattagtcgt tatgaacata cctaaatttg ctattgacca caatatacca
     9781 gtaattaaat tgaataaaac attttcccta tatacaatct ctttagaaaa atacttgaca
     9841 ataatagatg aagttatcaa cataccaaca gattgaggta atataattct atatccttca
     9901 attaaaaata tttgattagt tacaacatat aatgataaaa atagagatga taataataca
     9961 actccaacac attttaacat atttttatct gtagattttt tagtttgata atttgtgaga
    10021 agtataccaa gtattataca taatattgat aaagaaccaa tcagtaacat tttagaactg
    10081 gtccattcat taaacaagat tacagctaat aatgtcgtaa aagttaattg tgaaccattt
    10141 gaaattggca ttactttaga tacactagca atttgaattg ctttaaactg cattaattgt
    10201 cccaaactcc agaatatgcc agaaatcata cttatacaaa atgaaaaaaa tgtaatttca
    10261 ggatgtataa ccagagttat tatagtggaa aatataaacg cacttatgga tgtacctaaa
    10321 agctgttcat gaggagaact tttctttaga tttgcaacta tgggcatcaa tccccatccc
    10381 attacaggaa gtaaagcaat aattattgtc agcattaaac tatttccttt catattaaat
    10441 ttagacagct tttaatttat catctaaaat taaagtgttc aatacataaa aattaccgta
    10501 tttaatcagg aaatattagt agttttttag aaaaacattt tccattaaaa taaagacaaa
    10561 gggaaggagt aaaattgaaa atgttaactg atatgattca aaatcgtgaa attaaaataa
    10621 taaagttttt attaaaagaa ggtcatacaa ctgtaaaagc aatagcagat gatttagatg
    10681 tttcagaaaa aacagtttca aaatctctta aagaagtaga agcagtgctt aaaaagatag
    10741 gtctttcttt aataagaaaa cctaagatag gtgtttatat tgatggagat ttaaaaaaaa
    10801 tacttcctta cctagataat aaaggaagtc aaattcctac aaccagagag gagagagtta
    10861 tctatatata ttcagtactt ttaaagagta atgattatat tactatacaa caactttctg
    10921 atgaactata tataagtcgt ggaactattg aaagggattt agcagaagtt gaaaaactgc
    10981 taaaaaacga agggatttcc atatataaga aacctagtaa gggaataaag ttaaatataa
    11041 gtgaaagaga taaaagacaa cttactgcta agtttataca taaattttgg agtagaaact
    11101 ggtatattaa gcaagagcaa gaaagctttt atcaagcatt tgaaaagata caagcagatg
    11161 taaatggtat tttttcggaa gatgatttat caataattat tgatattctt aggaatttca
    11221 gcagagaaag aaaatttgag ttctctgatt atgaatttca gtcattagtt attcatttgg
    11281 caatagctgt agagagaatt agaaaaggcg aatatataga agaaagtttg tatactggta
    11341 atatagaaaa taatcaaatg tcaaatacca attatttagc aaaacaatta gaagaaaaat
    11401 tttcaatatt attaccaaaa tctgaaattg gatatataaa tttacattta gtagcgtctg
    11461 accaacatag agatactaat gaactaaaaa atacttcaac taatccttgt gagacaagta
    11521 caaataatat atcaaatctt cgggaaatta taaaaaatac attgtatgag acagcttatg
    11581 acaaggattt aattgatgga ttaacttcac atgtacattc atcaataaat agactgaaat
    11641 atggacttag tattaaaaat ccatatgtaa atacaatcaa acaaaacttt tctttatcat
    11701 ttgagcaagc tttagattta aagaaagtaa ttgaatttat atatgatatt aggataaatg
    11761 atgatgaatg tgcttatata gcattacatt ttgaagcttt tagagaaaga atgaaagaac
    11821 ttccacaaaa tattagagta attctagtat gcagtacagg attaggttca tctaggttat
    11881 tgtctgcaag aataaaaaaa tactttccaa tgataaagat tgaaaaagta ctttccgtac
    11941 aagaattagt atccagtaaa attgatgcag atttagtaat aagtactatt tatttagagt
    12001 tagaaacaat tccaaccata gtagttagtc ctattttaaa tgaaagtgat ataagaacct
    12061 tggaaagaaa tatacaaaat gtttcaagtt atacttctaa gaagtataaa tctttcagaa
    12121 acttaatctt tgaagaaaat atattgttga atattgaagc ttcaaactat gaagatgcaa
    12181 ttaagtatat aacagataac ctaataaaaa aaggttttgc aaaagaaggt atagctaaaa
    12241 gtgcaattga gagagaaaat ttatcttaca cttctttcca atcaatagct acacctcatg
    12301 ctaatcctag ttatattaaa aaatctaata tatctgttgc aacgttaaaa aatcctatta
    12361 catggggaaa tgaaaaggtg gaagttattt tctttatttc attagaaaat gacaaaaacc
    12421 tagagcttga tgaaatatat gaagtttttt atgacttcat tgacaataaa aaaaatataa
    12481 atgacatatt aagtgcaact tccacaaagg aagtgtacga tttgctagtg gaggagagta
    12541 tatagtgaaa atatcagatg tattgaaaaa agagcaaatt gtttttaatc ttgatacaaa
    12601 tacaaaggaa gaaacaataa aaaaattaag tagtatattt gttgaaaatg ggattgtgga
    12661 caatgaagaa gaatatgtta agtctgtttt ggaaagagaa gaacattcaa ctacaggtat
    12721 tggtaatggt attgctattc ctcatgggaa aagtaatgct gtcaaaaaat cttctataat
    12781 atttgcaaag actaagcata agattgaatg ggatgcttta gatgaaaaac cagtagatag
    12841 tattttctta ttggctataa gcaatattga taaggatagc aatcatcttg tactattatc
    12901 taaattagca actaaactta tggatgatga caatgtagat gcactaaaat tagccaattc
    12961 agaacaagaa ataatcaata tatttagtga agaaggagag atgtagaaat gaaaagaaaa
    13021 attatagcag ttacagcatg tgcaacagga gttgcacata catatatggc agctcaagcg
    13081 ttaaagaaag cttcaaaaaa gatgggtcac cttataaaag ttgaaacaca aggagctaca
    13141 ggaatcgata atgagttaac tacaaaggat gcacaaattg cagaagtagt aatatttgca
    13201 gtagatacta aagtcagaaa tgctgaacgt tttgagggaa aagaaatact agaagtacca
    13261 gttaatgcac ctataaagga tgcagagaaa gtaataaatg atgcactaaa gttaattgac
    13321 actaaataaa tggagggaaa tgtaatgttg aaaaagatag tctctgagtt aaaaaagcat
    13381 gtcttaactg gaatctcata tatgattcca ttagtaattg caggtgctat gataatggcg
    13441 atatctcgtg taggtggttc tttctataat ataccagata tatgggatgc taaatatgca
    13501 gaaagtgcta gtagtattgt tagattatta catgatttag atgggtttgg aggtactgca
    13561 ttaggattaa tgtttcctgt aattgctgca tttataggat tttctatagc tgataaatta
    13621 gctattgtac caggtcttgt tggaggtatg attgctaaag atataggtgc aggattttta
    13681 ggagctttgg cagttggtct tatagcagga tatacatgtt tgtttattaa aaatcatgtt
    13741 aaattaccaa aatcagcagc ttcaatagta ccaatattta tagtaccagt atttgggaca
    13801 ttaatcactg ttgtattaat caattatgtg ataggtatac cttttgccac attaaatact
    13861 ggattagaaa attggttaaa tggattatct ggtacaaatc agatactaat ggcagctatt
    13921 ataggggcta tgatggcagt tgacttaggt ggaccagtta ataaagcagc tttaacaaca
    13981 tcacttgcat tattaacaag tggaatttat gcgcctaaca ctgctgcaat ggtaggaata
    14041 gttataccac cactaggatt gggacttgct actatactag caaaacaaaa atataataaa
    14101 cagttacgtg aagctggaaa atcatctctt ataatgggtt taattggagt aagtgaagga
    14161 gcaattcctt ttgcggtaga atctccatta aaagtaatac cttcatgtgt aattggaaca
    14221 gcaattgctt cagctatggc agttggttta ggttcagtaa atgcaactcc aattagtgga
    14281 ttttatggat ggtttacagt agagaattgg ccaatgtatg tgttatcaat agcagttgga
    14341 acagtaattg tagctggatt agttatagta ttgcgtggaa atccagtgat tgaggatgat
    14401 gaatttgaag atgatgattt tgatgaagca gagtgggaat cttaaaattt aataactatt
    14461 taagacgtgt tataagctat attcaatatg aatcttatac taaataaagt gttaaacatt
    14521 cagtttagac ttaaatataa aagataaaga acagaggaga ataaatgata aaagcacata
    14581 ttgttaatca tacacactgg gatagagaat ggtattttac ttctggagat gcattagtac
    14641 ttagtgaaca actatttaca gatgtaattg atgaattaga acgtaatcca gatgtaagtt
    14701 ttgtacttga tggacaactt tctattttgg atgattatgt acaattacac aaagaaaaaa
    14761 tagaaatcat taaaaagtta ataaaagaag atagactaca tattggacca tggtttactc
    14821 aaactgatgc attctttgct agaggagagt ctattttaag aaacttaatg attggaattt
    14881 ttgaaagtaa aaagtatggt aagtatatga aaattgggta tttgccagat acatttggat
    14941 ttaatgcaca gatgcctata ctattaaagc atgtaggtct tgataatata ttcttttgga
    15001 gaggtataca tcttggtaaa caggttcaat caccatattt taagtggaaa agcttaaatg
    15061 gagaaagtta tatatatgca gtaaatatgc cacatggata tggaactggt atgcttttag
    15121 agccttcatt aaagtatgtt aatggacgct tagacccagc tattgatttt attaaatcat
    15181 atactgatgt agatgaggtg ttaatacctt ctgggaatga ccagttaaat ataataggtg
    15241 atttcaaaaa taaaattaaa gcgattaatg atatcggaaa gtattcatat ataacgagtt
    15301 catatcaaga gtttttaaaa tatataaagt ccatagaatc actagaaagc tatagaggag
    15361 aatttagaga acctgtatta gcaagaatac ataaatcaat tggttctagt agaatggata
    15421 taaaattagc atgtgataaa ctagaaaaca agctgattaa aagaatagag ccattattag
    15481 taattgctaa aaagagtaaa attgaaataa gcaatcagtt acttataaat acatggaaaa
    15541 aacttttgga aggacaagca cacgatagtt tagctggatg tgtaactgat actgtaacag
    15601 atgatattct tcaccgtata agagaagcaa atgaaatatg tgacagtatt gaaaatacta
    15661 ttgtaaaaaa gatttcagaa gggcttaaat tatctaaaaa tgatatttta gtatttaata
    15721 cagaagctaa gcgttttaat ggatataaag agatacaagt tgtaagtgat agtaaaaata
    15781 tttattttaa agataattta gatgctacta taattgaaga aacttatgta aaaccgagag
    15841 aaaatgtatt agaagaaaca cctgctggta atatatttat tgaagaacca ggatattata
    15901 tattaaaagt aagaataaat gtacagcttc ctgcacttgg ttataaagtt gttagttttg
    15961 aatcaagtga taaagaaatg gtgtcactta ataaatctaa tgatgctttt atatctaatg
    16021 ataattataa gataatatat gaagatggaa gcttaaattt acaacttaaa gatggaactt
    16081 acatatcaaa ctttttaaca ttaaaagata gtggtaatgc aggtgatacc tatgactttt
    16141 ctccactaaa aaatgatgag gatataaaac tttcgtttga taaagtgtat acagaaaaag
    16201 ctttaggata tgaaaaaatg gtggttaaag gtactacttt actaccactt acgttagaag
    16261 atagaaaaaa caaatatctt aatgggaaat taaatgtaaa agtaagtatt gtcttatcca
    16321 aagaaaatcc attgatggat gtaaaacttt gtgttgataa tacgatatat aatcatagac
    16381 tacgtgttca tataaaaact gatataaaag ataataaaaa catagcatca ttgccatttg
    16441 gatatataac aagagaaaat ggagttttag acaactggga agatatttac agtgaaatgc
    16501 ctatagattt agaacctttg gaaagtaata ttactctaac aaaccaaaat agaagctgta
    16561 ctatatttac taaaggaata aaagagtatc aacatattga agatgaaatt gcattgacct
    16621 tacttgctac tacagatgaa ctaggaaagc ctgatttatt atatagacca ggacgtgctt
    16681 ctggtgatac aactaaaaaa ggtcacataa gaattaaaac agataaagct caaatgctta
    16741 agaaattaga attttcattt gcaatatata tggattctaa atcatttgat gaattggaaa
    16801 ttgcgaattt aacccacaat tatctaaggg aatgtgtaaa ttatcaatta caggattaca
    16861 atttcttttt atatcgtata gataataaga ttcaaaaatc aattgtcaat aaaactgtaa
    16921 gtagagaatt agaaataata tctttaccag aaaactatct tatatcagca tgttatcctt
    16981 cttattacag taaagataaa tttataataa gagtggaaaa tccaagtagt aataaaatga
    17041 tattggatga gcaaatattt aaaaatagaa atgggaaaat tgtaaatgca atagaagatg
    17101 tagaagagga acaggtatat gaaatagcac cttttgacgt tatttcaata ttattggatt
    17161 tataagtgta aaagtaagtt tattgtttaa agtctgacag atattaatct gccagacttt
    17221 ttaatttgta taaatttggg taaatattaa tgaatggttt tagtatttaa gtatgaattt
    17281 agtatttaat atagaattta ttgaagtaga at
//
LOCUS       DAYATN010000076        17263 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.076, whole genome shotgun sequence.
ACCESSION   DAYATN010000076 DAYATN010000000
VERSION     DAYATN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.076"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(114..638)
                     /locus_tag="ACGI8A_002348"
     CDS             complement(114..638)
                     /locus_tag="ACGI8A_002348"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFH0180344.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(703..1455)
                     /locus_tag="ACGI8A_002349"
     CDS             complement(703..1455)
                     /locus_tag="ACGI8A_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFH0180345.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1486..2784)
                     /locus_tag="ACGI8A_002350"
     CDS             complement(1486..2784)
                     /locus_tag="ACGI8A_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HFH0180346.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2801..5065)
                     /locus_tag="ACGI8A_002351"
     CDS             complement(2801..5065)
                     /locus_tag="ACGI8A_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HFH0180347.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5320..6138)
                     /locus_tag="ACGI8A_002352"
     CDS             complement(5320..6138)
                     /locus_tag="ACGI8A_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFH0180348.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6131..6829)
                     /gene="cbiQ"
                     /locus_tag="ACGI8A_002353"
     CDS             complement(6131..6829)
                     /gene="cbiQ"
                     /locus_tag="ACGI8A_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HFH0180349.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6832..7116)
                     /locus_tag="ACGI8A_002354"
     CDS             complement(6832..7116)
                     /locus_tag="ACGI8A_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0180350.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7106..7858)
                     /locus_tag="ACGI8A_002355"
     CDS             complement(7106..7858)
                     /locus_tag="ACGI8A_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFH0180351.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8040..8232)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8364..10136)
                     /locus_tag="ACGI8A_002356"
     CDS             complement(8364..10136)
                     /locus_tag="ACGI8A_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HFH0180352.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10150..11079)
                     /locus_tag="ACGI8A_002357"
     CDS             complement(10150..11079)
                     /locus_tag="ACGI8A_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0180353.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11300..11485
                     /locus_tag="ACGI8A_002358"
     CDS             11300..11485
                     /locus_tag="ACGI8A_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFH0180354.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            11478..12179
                     /locus_tag="ACGI8A_002359"
     CDS             11478..12179
                     /locus_tag="ACGI8A_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0180355.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12181..13104
                     /locus_tag="ACGI8A_002360"
     CDS             12181..13104
                     /locus_tag="ACGI8A_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0180356.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13261..14184
                     /locus_tag="ACGI8A_002361"
     CDS             13261..14184
                     /locus_tag="ACGI8A_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180357.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14177..14914
                     /locus_tag="ACGI8A_002362"
     CDS             14177..14914
                     /locus_tag="ACGI8A_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180358.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            14925..15698
                     /locus_tag="ACGI8A_002363"
     CDS             14925..15698
                     /locus_tag="ACGI8A_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180359.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16239..16847)
                     /locus_tag="ACGI8A_002364"
     CDS             complement(16239..16847)
                     /locus_tag="ACGI8A_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFH0180360.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17025..17141)
                     /gene="rrf"
                     /locus_tag="ACGI8A_002365"
     rRNA            complement(17025..17141)
                     /gene="rrf"
                     /locus_tag="ACGI8A_002365"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5555 a         3000 c         2191 g         6517 t
ORIGIN      
        1 aaaagtgaaa tgtatttgtc taaaaacata ataacatttc actttatcct catttttaag
       61 taatatatat ttttaatttt tatttataac taaatctagt ttttatataa attttagttt
      121 ttttctacat ctgagtaatc tattccaaaa gtttctacag taacagattt cataacttgt
      181 ggagtttgtg gcttatcagc tgaatctgtt ctaactgatg ctatttcatc aactgtatcc
      241 ataccttcta tcactctacc aaaacctgca tactgaccat ctaagtgtgg tgagttttta
      301 tgcattatga aaaattgaga accagctgaa ttaggtgcca tagttctagc cattgatatg
      361 actcctcttt catgttttaa attgttagta aatccattac cactaaattc accttttatt
      421 gagtgacctg gaccacccat tcctgtacca tttgggcatc ctccttgtat cataaatcct
      481 ggtattactc tatgaaatat tattccattg taatatcctt catttactaa tgatacaaag
      541 tttttaactg tatttggagc tatatttgga tataactcaa ttttgatttc ttttccattt
      601 tccatttcta tagttactat aggattttta ttttccataa tttattacct tccttttcat
      661 tagttaaatt acataattac atttattata ttatactcta aattaaagtc tatcaagtat
      721 attttttcct atttcatatc cttcttgata aatagtttta caaaactcat catcaaagtt
      781 aagcgtgtct ttaggagcaa attgagtatt aggtctaact actattattt tatcttcatc
      841 atatactttt ttaatatctt ctggcaactc ataattatat tttgtggata ttaaaataat
      901 tttatccaca ttatccccaa ttaatggttc aattaatgca ctcctaatta aacctccatc
      961 taatttgaaa ccatcataaa ttccttcttg cacatctttc acaaattgtt ctttaaatcc
     1021 aagagttgcg tttggatttg atggtaacaa cccactgtat gaaatgctga taatggcttc
     1081 attacgaggc atttttgata cgtcaacaat cttttctgat actactttat tatcaacttc
     1141 aacataatta acataaaagt tcatttcttc tatattacta tcatctagtt cccttaggtt
     1201 gtctatagca ggagaattat ccacagtatt atttactatt tttacatttc cattctggat
     1261 atcttttata tttgtccaca ttttctccaa gttatcaaca ttccctgtgt acaaataata
     1321 tccatttatt gctcctattg atgtccctga aatcgaataa aatttattta ttcctccatc
     1381 atacaaggct ttaactactc ctgcttgata agcccctttt gctcctccac cttctaaaca
     1441 taatcctaat ttcatagtct catctccaaa ttgtttatct tatatttata tttcaaaaaa
     1501 cttactaaat gttttatata tactatagtg gtcctcaaca tttcctaatt ctcttataga
     1561 atgcattgct aaaattggac ttcccacatc cacagatggt atatctatat gagtagaaga
     1621 tattggacct atagtacttc ctcctctttc atctgaccta ttaacaaatt cttgatactc
     1681 aacattagct tctctacata tatttttata aatagctata gaatatccat cactagtata
     1741 tgcctgacta gcatttatct ttataaccgg accttttccc attattggtt tattagttgg
     1801 gtcatgcttt tctggaatgt ttggatgaat agcatgagct aaatctgatg atatcataaa
     1861 agatgaataa atagactcaa agaatccctc tctatcctta cccattgata tgcaaattct
     1921 ctccaatata tttaacaaca tgtttgaatc tgcaccttgt ttagtagagc taccaacttc
     1981 ttcattgtca aatacagcca ttacatttac accatgttga cctttagtat taacaagagc
     2041 attcaaacta gcatgtacca ttgcaagatt atcttgtctc ccaatagata taaattcatc
     2101 attagcccct attaaacttc ctttttcaaa ttcatataaa aataaatcaa aatctataat
     2161 atcgtccata tttacatcta agttagatgc tatttgcttt ataagaaaat tatctttttc
     2221 taatgtatca tttaacatcc caactaaagg taacatatct ttttgcttat tatatgagtg
     2281 accatcattt atacttctat ttaaatgtat tgctagattt ggtattatac aaattggctt
     2341 tttaaagtct ataagccttt catttggttt taatatacta tcacctttta aaactactct
     2401 accagctatc ccaagaggtc tatccaacca tgtacttaat attgctcctc catagcattc
     2461 tgtatttaac ttcaaataag atttttcagc ttctatttct gcatttggtt taatactaaa
     2521 tgtaggagaa tctgaatgag agcctattat tctaaatcca tctctttcta taccttctga
     2581 atttactaca aaagctacta tagctgataa attcttagta acataatact ttccaccaac
     2641 tcttagattc catctttgat tcaaaactaa ttcttcaaat ccatttttat ctaagatttc
     2701 cttagtactc tcgactgcat gaaaagacga cggactatca tatatgaagt ctattaagtt
     2761 tttagcaaag tttatactat ccattcctca tttacctcca ttataaaata tttagtgttg
     2821 tatattcttt atttattttt ttaattccct gttggtcaaa agctatagta acatctgtac
     2881 caattataga aaccactgtc ccaaccccaa atttaggatg atgtacctta gaaccaagtt
     2941 ttatatcatc tggattagtt tctttattag aatcttttat tgtagcatta actttatctg
     3001 caactttaga tttgcttata gtactcatat actttttagt atatttatct aacatattat
     3061 aacttgcttt agaatagctt agttcttttt ttactttgta caatctttct atacattctt
     3121 ctggcagttc ttccataaat cttgaagcta ttgcaacatt tgttttacca tataatgttc
     3181 tcttctctgt caatgacata tatagctctt ctttagctct agttatacca acataacaaa
     3241 gtcttctctc ttcttctatt tgtgaatcac tcatagattt tatagctctt gatataggaa
     3301 ataacccttc ttccatacct gttaaaaaca caacagggaa ctctaaacct ttggatgtat
     3361 gaattgtcat aagcgaaacc ctatcattgt cttcttcatc atttgattca gatgtaagtg
     3421 ctatacttgt taggaatgtc tctaagtctt tattttcata agtatcatca ttactacttt
     3481 caaactccaa agctatagaa ataaattctc tcaaattatc aattctgtct tgagcttctt
     3541 cacctttgcc tgttaaatct ttttcatttt tatttcttat ctctacaagc tcgtccatat
     3601 atcctgtagt atccaagact ttttctataa gtttgctaac aggataaact tcttttatag
     3661 ttctaagagt ccctataata tcaacaaatc cacttatact tgcccttgcc ttagtagata
     3721 tatctgaatt tgtttctata tcaattaata ctgagtatat actttcctgt tttaagttag
     3781 ctctatcttc aattttttct atagttctaa gccctatacc tcttctagga acatttataa
     3841 tcctttttat agatatatca tcttgtggat tttgaataac tcttaaatac gccaacaaat
     3901 cctttatttc ttttctctca tagaattttg taccaccata tatattgtat ggaatttgac
     3961 ttctatttaa agcgtcctct acaggtcttg cctgtgcatt tgctctataa agtactgcaa
     4021 aatccttata aggtctattt tcttttctag ctatttgtgc aattgaatca gcaatgaaat
     4081 cagcctcttc tatttcacta ccagttaatt gtatctttat aagttctcct tctttttttt
     4141 cactccaaag cttttttctt tttctttcta tattattaga tataacttta ttagctgcat
     4201 ctaatataac ttgtgtagac ctataatttt gttccaactt tacaacatgt acattgtcat
     4261 aatctttctc aaattcaagt atgtttctta tatctgcacc tctccatcca taaatacttt
     4321 ggtcatcatc tccaacaaca caaatatttt gatgttctct tgctaataat tttatcaatt
     4381 catattgagc tttacttgta tcttggtact catctaccat tatgtatcta aatctacttc
     4441 tgtaatatgc taacacttca tcattagcct tgaaaagttc tacagtttta agtattaaat
     4501 catcaaagtc aagtgcacta tttcttttta aacggtcttg atatagcgca taaatatctg
     4561 caatcttaac cattctatta tctgccatat tgatatcttt aaactgcttt ggagtataaa
     4621 gtttgtcttt tgctcctgaa attgcactta taactgcttt tggttcaaac actttatcac
     4681 ttaaatttaa ttcttttaga cagtctttaa ccaatgtaac ttggtctgct gaatcataaa
     4741 ttacaaaact tctattgtaa ccaatcttat ttatatcttt tcttagtata cgaacacagc
     4801 aagaatggaa tgtacttatc cacatatcct tagtttcagg acctatattt tgttcaactc
     4861 tctctctcat ttcatttgca gctttgtttg taaatgttat agctaatata tttggtgctt
     4921 gtacaccttt atcttctata agatatgcta ttcttgttgt taatacctta gttttacctg
     4981 aaccagcacc agccagtata agtactggac cttcagtctt ttctactgct tctctttgag
     5041 caggatttaa tgtatctaaa ttcatatttc tcctcctgtt aacagccata aatatatttt
     5101 aatctaaaat atgaattact tcaaattata attatgtatt atattaatag attatttcca
     5161 atatattaca attaaataac atttttccat ataatttatt tcattgttta ataaaaaaaa
     5221 taaccttatg atttggggaa tcataaggca cctttaaggg gtctaggtta agggttatta
     5281 tatataaatc tattttttta aaataggttt agggcttaat tatttatttg tattaagttc
     5341 tctttccttc caatacttat acaaatcttc ttctttctta aataagggta aattcatatt
     5401 tctatgtact tttactaacc atggggattc taggccagct tgtataatat tattttcatt
     5461 tataaacaca ttttctgctt ttccttcatc aatcaaaatc cccttatcca atacatatat
     5521 gtaatcacat atttcataca ttaaattcat atcatgactg gatattacta tttttatatt
     5581 atttttattt aatcccttta ttatatctac aatagaccta gtacttactg ggtctaaacc
     5641 agctgtaggt tcatcaagta atacaatttc attttccatt gctataactg atgctattgc
     5701 cactcttttt ttctgtccat agctaagaaa atgtactggc ctgtctataa aatctattcc
     5761 attaacagct actagagctt tgtttattct ctctttaatt gttttttcat ccataccaat
     5821 gtttctcatt gcaaaggcta tatcatcata tacttttgaa taaaaaattt gtttttctgg
     5881 gtcttggaag acaataccta cattttttct aaggttgtat aaacttctct tatcatattt
     5941 taacttttct tctttaaaaa gaatttctcc agatgtaggc tttaatatac ccattaaatt
     6001 cataaataat gttgattttc ctgaaccatt tgaacctatt atacctataa catttccctt
     6061 tgaaaaatcc atattaatgt ttaatagtgc attggtattt ttttcatatt gatacgttaa
     6121 attatttatt ttaaacatca ttatccccta ctatgtgaaa tttaccatca tacaatttta
     6181 tgtctaatga tatacacata tcatcatacc ttgtcataag tcttttgtac agcatacttc
     6241 caagaatacc taaggattta tatgaagttt ttaaatttat atatccaaat cttagctctt
     6301 gtgacttgta tatttctgaa aattcttcta aaaatatgaa tataaaccta tatattaaca
     6361 ttgataactc gacaaaagtg tcaggtaaat gaagtttttt aaaaaagaat attaattgat
     6421 taaaaggtgt tgtaagtata caaaaataca cacaagtcaa acaagacatc gctctaaata
     6481 acacatgaat agacatgtta atagacgcct ttgatattcc tacattaaaa ctaaaaaatt
     6541 caaaactata taataaatct gctttattaa aagatatatt tacaagagtt attcctatac
     6601 taagaaataa aaagtacatt ggtattctaa gtaattttaa ataatttttt aaatctattc
     6661 cagccacaca aacaaccaaa atactcatta aaatcataat tccacttaaa ataaatatat
     6721 tttgtattac catagaagca atcagaaaaa ttactcctat ggctactttt ttgtttggat
     6781 ttactttact caatctattt gtatatgcat atttatcaat tataagcatg attaacttct
     6841 ctttcctttt tgaagaccta atatgtatcc tattacacca gcccctaaag ctgcttgtgc
     6901 agagaataat aaactttcta tttctccact tggtggctca tatggactgc taaaccatgg
     6961 tttgtaattt ggatttatct tagtaatttc actttcagct tgaccatctg ctcctccata
     7021 ttcagcacct gagttgacaa gtaatgggaa tataataaga gctactacta ataaaagtaa
     7081 taatatgtta gtttttgttt ttgttttagc actcattagc tttaactcct ttatcattat
     7141 tttctagtat gcttttttct ccgttttctg taattaaatt atatacaatt acagtaagta
     7201 taccttctgc tattgctatt ggtatttgag tcataaagaa aattcctaaa aatttaattg
     7261 ctgaagccat taccccacct gatgggtctg ggaaagccag tgctaattgt attgaagtta
     7321 ttgtatatgt ggctaagtct cctattgctg ctgcaagaaa tactggaata gcattatttc
     7381 catctttttt ctttaaagcc ttaaatatta ggaatgatac gattggccct attatcgcca
     7441 ttgaaaaagc attagctcct aaagtagtta ttcctccatg tgctaataac aatgcttgga
     7501 atattaaaac tatagttcca agaacaaaca ttacgcttgg tccaaacatt attgctccaa
     7561 gacctacacc agtaggatgt gaacaacttc ctgttactga aggtattttt agtgctgata
     7621 atacaaacac aaatgctcca cataaagcta ataatacttt ctttttaggg tcttgcttta
     7681 ctattttacc tattgatttt aatccgaata agaaaaatgg tataaatata actccccatg
     7741 ctatagacca ttttactggt aagtaacctt ccattatatg cattgcattt gatgtaactg
     7801 gagttaaaat gattaacata agtgcagcta taacccctaa tttaatattc tgtttcatct
     7861 tagtatccct ctctcccttt ctaataatta aaacataata atacatgtct gatttattta
     7921 ataaaataaa aaagcctaac tcgatggtta ggcttctaat tttccacaat ctaaatacaa
     7981 acacataaaa caaacatatc tgtataatta ttataagaaa ctttctccat ccccgaagaa
     8041 attactttaa tattttaggc aggtctcctg acttagcttc atcctactcc ttttccttcc
     8101 catgagatat aaaaaatatc atcatagtgg ctaacaaaag tttcgtctgc atcacagtag
     8161 cgggggctgt aaaggaattt aacctttttc cctattaatc ttgcttaaag aacctataat
     8221 attatatatt taatttttaa attattctta aatcattttc tatataaatc attgtatacc
     8281 ttttctgtga aaatttctac attttttata aattttattc atttttatat ttttatgtat
     8341 ataaattctt ttttgtaagt ttattaattt ctaattatct tctttaaata cattgaaact
     8401 acatcagaaa atctagttat atctcttgaa tatataaaat catttccata tattaatctt
     8461 tcagcttcaa gttctgattc ttttcctgta aatactccta atactaatat attttgcttt
     8521 ctagcttttc tcacttcatt ggctgtatct ctcaaaccaa ctatacccct ataagccttt
     8581 tctcctctta atgttctctc actacttttt cctatcttta catcatttgg tcttccatcg
     8641 ctgagaacta tcaggatttt attttcttca tctctattta ttagaccact acatatagat
     8701 tttattgcca aaccatctct attattacca gcacaaaaat attcaaatat attttcacta
     8761 ttttttatac tatcatcata atctttaaat ctttttaata ttgtatagtc taaaaaacta
     8821 gaaaatccca tcactctatt tggtatgcca acttctgtta gagcatttgc aattatgtaa
     8881 gcttgaattg ccacatttcc ctgatttcta ctttgagagc cacttgcatc caataatata
     8941 tcaataacgt atttcccttt ttcattttct atatctttat aaaatatttt attattatta
     9001 cttctaccaa ctctccaagc tctattggct aatatagtac cacaatcaga atatactcta
     9061 cttttagcat tttcttctat taaaattctt gacagacttt cttttaatct ctttatttgc
     9121 tgtctataca ttctagcatg ttctctaaat tttcctaaat tattttcttt ttgtcttgtt
     9181 acatatttta gttgaaatac attattacac tcacttctaa gaactccatc tgtaaagtgt
     9241 attctacaac cttcatgaac atttctacaa ttttgtgtct caattttttt aatttcacct
     9301 ttactcaaat aagtttttcc ataataatgt tctatttttg aatatatctt atttgcagtg
     9361 tcttcatcaa catatataac tcggtctgct gaattgctac atttagaatc atcaaaatcc
     9421 cctacacctt ctacaagcat ggatgaagat attgaatcta tatcactttc aataatattt
     9481 tcttcttcat cactataaag ttcttcatac atgaaatcag caaatgtatc aaaatttact
     9541 tctacacttt ctacattatt ttcatcaata agactatttt cttctccatt taatatgtat
     9601 tcaaaataag ttaaatatat aacatctaat gcttttaaaa tctccataat atcttgattg
     9661 gtatctatat ttcttatatc attaactatt cttctagtca tactatctaa tgctgggtgc
     9721 ttgtccatac tataaagtac aaatgtgtat ttaattttat ccataatatt atcattgtgt
     9781 attttggtaa aacgtgaaac aatatcttta taagcattcc ttcttatgtc ttctatccct
     9841 ggcctgtctt taatgacctt ttcttcaact attgtattta atataacttg aattagacct
     9901 agtataacat ctttgttgta tccttttctt attctatttg taagatattt tttaactata
     9961 tcccaatcta catactttct tctagcacct gcaattattg cgaaatatag acctatttct
    10021 tttgaagagt aatctttttc tgatatatct atactttcat tgtaatcacc agctattgtc
    10081 catgataggt tattagctct attttcaaat tcataatcat catatatcca tttcataatc
    10141 tcacctatat taaatatgac ttattggaaa cacatctata ctctcccatt tattaggtat
    10201 tctagtttta attacatctc caaccatttc tttctcatat acatcaaaag tctttcctgt
    10261 aagtcccata ttaattgcaa gagttggttt taatcctctt cttatagtct taagagatgc
    10321 cataagacca cgtaaatcaa tagctttact agatatctct ccattttgag acttcaattg
    10381 taaatctaaa aatattcctg caaagtgtat taatttttct tcatcagcat ctgaaaattc
    10441 attttttaaa atcatcatta acttatcttc ttctatagga ggaatatcta ttaccataaa
    10501 tctagatacc agtgcttcat taagttcttt tgttcctgca tattcataat tcatagtccc
    10561 aataaatctt gttgctggat gtagattgac tctctcatac ccaggtacat caattattct
    10621 tctatagtct aaagctgaat gtaccacaac tatagcatca ttttttgcca tattaatttc
    10681 atcaaacacc ccaaaacctc cattaatagc acactcataa actgagcctc ttctaagttt
    10741 aacttcattg tctataaatg tatctgtacc tataagtgtt gaactgtctg tatttatatg
    10801 aaatgatgta ttccactgag gtctattaaa tatttcccct agattatcag caagtaagtt
    10861 ttttcctgtt gcctttggtc ccgataataa tatattttct tcttctaaaa tagctgctat
    10921 acacatagac aagatatctt taccataaaa ataagtttta gactttgtta ctctgtattc
    10981 taaatctttt tctaaaccat aataatttct aaaatacagt acatcttcaa taagtttttc
    11041 atctacccct tgcaccctta gattatctat tatatccata tttttacccc ttttatataa
    11101 atagtatttg tttcatttct tatcaccatt ttagcataaa aggatataag ctaaaattga
    11161 ttttttaatc tccccatttt aagtaaaacc atgcaccatt ttatcactac aaaattaaga
    11221 gtactactta ctgatatttc ttgattttgt tcgtctatac gtatataata tatgctgata
    11281 aattatgagg aaaggatgat tgaaatatgg atgcccagaa gtagcaaaaa aatggggtat
    11341 ttctgaaaga agagtacaaa aactatgtga agaaagtcgc ataccaggag tttcaaaact
    11401 cgggtatatg tggctgatac caaagaacgc agaaaagcct gttgatggta gaacaaaaag
    11461 aagaaaggaa gtacaatatg aatagcgttt tgattataga tgatgacaaa gaactttgtg
    11521 ctcttatgaa aaaatgtgtt gagcaagaaa atttatctgc tattactgca cattgtggtg
    11581 cagaaggtct acatttggtc gataaaaata gacacacact ctctcttgta attttggaca
    11641 taatgatgcc agatatagac ggttttcaag ttctgaaaaa aattagagaa acaagtaata
    11701 ttccagtaat tatgcttact gctaaaaatg acgaggatga taaagtttcc ggactacgtt
    11761 taggtgctga cgattaccta acaaaaccgt tcagcataaa cgaacttatg gctcgtgtca
    11821 attctcttat tcgtcggtat actatgttaa accctgcttt tataacagac acagattgca
    11881 taataataaa tggcatgatt attgacaaga caaaccgaat tgtttctgtc aataatattc
    11941 ctgttcaact tacaagcaaa gaatttgatt tacttgcttt tctggcttct aataagggac
    12001 gggtgtttac caaaaaacag atttatacac aagtatggga agaagaatat gcttttgacg
    12061 atagtaacat catgtctttc atcagtaaat tgagaaaaaa gattgaacca gaccccaacc
    12121 atccatttta tattttgaca gttcgtggtg tcggttatcg ttttaataag gaggcttaac
    12181 atgaatacaa gtgtctatct gcttttgata ttggttctta gcctgattat tatagcgtac
    12241 cttgtttctg tcctgctacg tgtccgaata caacttacac ttataaaaga tgctttagag
    12301 gatataaaaa atggaaacct taatcgacgt attttaacag atgaaaacga cattacaaaa
    12361 caaatttgct atgatattaa cgaaatagca atcaatagcc aatttcaact tattcagcaa
    12421 aaacaatcag aacaagccta caaacagcta atgacaagtc tctcccatga tgtaaaaact
    12481 ccacttgctt ctttggtcgg ttatttggaa gcagtagaaa gtaaaattgt tgtaggagag
    12541 gaaaaagacg agtatatcca tgttgcgtct aataaggctc attacttaaa gaattttgta
    12601 gaaaatctat ttgaatgggt aaaattggat tctaaggaac aagtttttca ttttgatatt
    12661 tttgacttga atgaattatc ccgtaatatt atatctgatt ggatacctgt tttagaaagc
    12721 agtcattttg aatatgaatt tgatatacca gaaatagagt acttcttacg tatagacgca
    12781 aatgcatata cccgtatcat taataaccta ttacaaaata ttattaccca tagtagtggc
    12841 aacaaaatga cattgagaat ttttgaaaac aaagagcaag ctcaaattat aataacagat
    12901 aatgggaaag gtatatcttc agacaatctc ccacatattt ttgaaaggtt gtaccaatgc
    12961 gaccattcac gagcttcaaa gggaaatggt ttaggcttgg cgattgcaaa ggaacttatt
    13021 aacgctcata agggaaatat taccgccaac agcactcccg gtatgggaac agaatttaca
    13081 atattgcttc ctaaagccct gtaaaagtac gaggctttct tactctaaaa caagataaaa
    13141 gcaagattat ggcaaggttt tggcaaggtt catgttttat aattctaatt gtagttgatt
    13201 ttgacgttgt atcaactatc ttttgtagaa acaaatttat taaaagtgag gaaattatat
    13261 atgaatgatt ttgtgattga aacaaagcaa cttacaaaaa tttatgggaa tcaaacagta
    13321 gttgacaaag tcaatctcca tgcaaaaaaa ggccgaatat atggtctgtt aggacgcaac
    13381 ggagctggga aaaccacaat tatgaaaatg attttaggat tgacttcaat cacgtatggc
    13441 gaggtagatg tatttggaga gaatattaag ggacgtgaaa aacaagtata tccccgtatc
    13501 ggtgcaatta tcgaaacgcc aggattttat cctaatctga caggaacgga gaacttggag
    13561 atttttgcaa aactgcgtgg tacagccgcc cctaattctg taaaaagagc attggaaatt
    13621 gtaggcttac cttataaaaa taaaaagctg tttagtgaat attcacttgg catgaaacag
    13681 cgacttggta ttgccaacgc tatcctgcat gacccagaac tgttagtttt agatgagcca
    13741 accaatggtc ttgaccctat tggtattgcg gagatgagaa agtttattag ggacttgagc
    13801 gttgagcatg gaaagacaat tcttatttcc agtcatattc tttcagaaat ctcattgttg
    13861 gctgatgata ttggaattat cgaccacggt gttcttttgg aagaaagtag tatgagtgaa
    13921 ctagaaaaga aaaacagtaa atatatcctg ctacaagttt cagatattcc gaagacatca
    13981 cttattttag agcgtcaatt taacacgaag gattattccg ttcaagatga ctacacgtta
    14041 cacctttata acactaatct ggatatgggt gaaatcaata aagcacttgt aatgcagaat
    14101 gtaactgtta ttagttctca actttgtaat gatactttag aggactactt taaaaaaatc
    14161 acaggaggag agggcattgc ttaaacttat tcaaacagaa tttcttaaac tgcgtcgtag
    14221 gaaacttatt tggctgatgt tgttgtctgc tctcattatg ccgtttttcg cactactata
    14281 cttcaattat tttggtaaaa caggtgttga gccaataaag ttttataaat ggtctgcatt
    14341 tggttataca ctttggatta ttttgcctgt tgttttgggt atattttgca ccatgcttat
    14401 acacgaagaa aaccagtatg atatgctcaa acagctttgg attgtaccta ttagtaaaat
    14461 gggatacttt tttagtaaat ttttcgttgt tttgatttac tctatttgtt ttatgctgat
    14521 tacagccgtt gcttctgtgt tatttagtgt actttctgga tatgtcgtat ttagctgggc
    14581 aagcatttta tatttgttga aaaaatgttt agaaattggt gtaatcactg cttttgtaat
    14641 gttgccgatt ttggcaatcg ctacatctca aaaaggatac attctcccaa tttgtatcac
    14701 attggtatat gccttttccg gttttgttat aatgacaata aatatgtatt tacacccatt
    14761 atccagtatg ggagttatta tcatgagaaa taaagacatt ccaggtctta tattttcaca
    14821 ggaaattaat gtcccattag catttctttg tatttgtatt tggagtattg tatcagtatt
    14881 gtttgcaaac actgcactag gaagaagaaa gtgaggtgta gataatgtgg aaattacttt
    14941 gggctgaatg tcaaaaaatt cgtcgttcaa aaattgtttg gattacagtt tttgcatcca
    15001 ttatgatagc agtcatagtt tttgcaggag ggcaggatgt atataatgac ccagatttac
    15061 attatggctt aaagagcgtt tatgacggtt cacggtatat agacaatgca ggttggtata
    15121 tggacgcagt acaaccttgg tcaacttttt ttgttcttcc tgctgtcatt gccctattag
    15181 gaagctatat tatttgtcgt gaagaagagg aggacactat taaatccctg cgtcttattc
    15241 ccataaatga aacaaaatta accatagcaa aaatgattat tacttttata ttcagtatac
    15301 ttctttattt gttactcttt actataacat ttttgactga atccgtttta catttttccg
    15361 atttatcgac aaaactggtt ttaagttgca tgaaagagta ttttcttgat ggcataggtg
    15421 tattctttgc aatttctccc attattgcct tggtatcgcg catgaaaaaa ggatattggc
    15481 ttgcattagt attcacagaa gtttattcta ttgctggact atttgcgggt atgtcaaatg
    15541 tcttacaaac cttttttcct attgcggcaa tatttaatat ttctggatac tatataacta
    15601 ctggagaaaa aattatgggg agtatcataa gtttattact ttgtgcctgc ctttctgcat
    15661 ttatacttaa agggctaaaa cacaatgaaa aaaattgaaa taggtatatc aaaatgaaaa
    15721 agataaaaaa actgattatt ctaattgtat tcttgtttct ttttggcatg ttacactgtt
    15781 atactgaatg gaaattcaac aataacccac ctaaaggggt aatagaaaga ataaatgaat
    15841 ggactgataa gattggactg gattagtaac ttatgttaat tatagtgaat gtaatattag
    15901 caagttattg tttcgtattt taatactata actttaaata tttggtttta atcttccaaa
    15961 cgaatataaa aaaactgtca cagaggcaac atatttttga atatttataa agtggtagtt
    16021 ttgattttca aagctgccgc cttttcttct atataagcaa acttatcctc aacaaagtgt
    16081 aaaacatggt acattttaag aaaaaagtac cacagacaca agcaataaaa gaaacgccga
    16141 gaacctacaa aaacgtagtg cttatgcaga tttacgacgc cttctatttt ctcaaccgtc
    16201 ttaatgacct tgtacaaaag aaatttttct aatatttttt ataatcttat cttgtgatgt
    16261 tttttatgta taaatgactt aataacaaat ccaataaaaa ttatggatat tggtataatt
    16321 agaacgagga atttataatc attataatgt gacattattc ttacccaact ctcagaaaaa
    16381 atatatccta tactgattaa tgaagtattc catatggtta ttcccacgat agatgatatt
    16441 acaaaagtta taatattttg acctgctata cctgctataa aagatatgta tgttctacat
    16501 aaagggatta atctacatac acaaacagaa atagaagagt atttgttaaa ataatcttga
    16561 gatgcaaata ttcctttctt tgcctttgga catatctcta ttattttatt tatgatagat
    16621 ttacctcccc aatttcccac tgtataacaa aatatcgaac caagtatacc tgcaattata
    16681 cttaaaatta aaattgtcag aaatccaaaa tgatttcttg cagcaattgc tccacataaa
    16741 ggtaaaacaa cttcgctagg taatggaaaa caagcatact caatcataat taatacaaat
    16801 acagagatta atccatattg taaaataaaa ctattgatta cagacactta cttatctatc
    16861 tcctttctta tttaaaggtt ttaagggtac atgttctaag gattctcacc ctatatttaa
    16921 agaatcaatc tctttaaact attatatgta tttacacaaa gcatttataa catactttaa
    16981 aaatagtacc ttaaaataag aaacttaata aataaaaaaa gattacgtgg ctacgtccta
    17041 ctctcccagg cagtttccca ccaagtacca tcagcgctag agagcttaac ttctgtgttc
    17101 ggaatgggaa caggtgtatc ctctctgcta ttgtaaccac ataatcttta tgcttaatat
    17161 ttctattaag tttttaataa gtttatagaa cctagtaata ctagtttctt tagagttagc
    17221 actctaaaaa ctacacatat atcttaatga atgaacatca aat
//
LOCUS       DAYATN010000077        16939 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.077, whole genome shotgun sequence.
ACCESSION   DAYATN010000077 DAYATN010000000
VERSION     DAYATN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.077"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            269..1099
                     /locus_tag="ACGI8A_002366"
     CDS             269..1099
                     /locus_tag="ACGI8A_002366"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0180361.1"
                     /translation="MLNKLCMYLERPELYKQSEINFWDDEYISEQLLKAHLDTNFEGA
                     SRNFNFIEDSVNWIVTVANPVNYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSIN
                     YAQNRNKETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSL
                     KPGGKLILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTL
                     VITEKDENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK
                     "
     gene            1509..1643
                     /locus_tag="ACGI8A_002367"
     CDS             1509..1643
                     /locus_tag="ACGI8A_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180362.1"
                     /translation="MDLINTIDKVQQAHKPIDEIIETIRDIVDIIKILEPVYNFKSNQ
                     "
     gene            2148..2909
                     /locus_tag="ACGI8A_002368"
     CDS             2148..2909
                     /locus_tag="ACGI8A_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFH0180363.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            2974..3756
                     /locus_tag="ACGI8A_002369"
     CDS             2974..3756
                     /locus_tag="ACGI8A_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFH0180364.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKCKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            4023..4442
                     /locus_tag="ACGI8A_002370"
     CDS             4023..4442
                     /locus_tag="ACGI8A_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HFH0180365.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(4545..4811)
                     /locus_tag="ACGI8A_002371"
     CDS             complement(4545..4811)
                     /locus_tag="ACGI8A_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFH0180366.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            5038..5739
                     /locus_tag="ACGI8A_002372"
     CDS             5038..5739
                     /locus_tag="ACGI8A_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFH0180367.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            5821..6429
                     /locus_tag="ACGI8A_002373"
     CDS             5821..6429
                     /locus_tag="ACGI8A_002373"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFH0180368.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            6753..7217
                     /locus_tag="ACGI8A_002374"
     CDS             6753..7217
                     /locus_tag="ACGI8A_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HFH0180369.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTYETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            7854..8870
                     /gene="gap"
                     /locus_tag="ACGI8A_002375"
     CDS             7854..8870
                     /gene="gap"
                     /locus_tag="ACGI8A_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFH0180370.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            9265..10434
                     /locus_tag="ACGI8A_002376"
     CDS             9265..10434
                     /locus_tag="ACGI8A_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180371.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYSPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            10814..15067
                     /locus_tag="ACGI8A_002377"
     CDS             10814..15067
                     /locus_tag="ACGI8A_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HFH0180372.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            15216..15710
                     /locus_tag="ACGI8A_002378"
     CDS             15216..15710
                     /locus_tag="ACGI8A_002378"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFH0180373.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            15826..16203
                     /locus_tag="ACGI8A_002379"
     CDS             15826..16203
                     /locus_tag="ACGI8A_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HFH0180374.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            16409..16849
                     /locus_tag="ACGI8A_002380"
     CDS             16409..16849
                     /locus_tag="ACGI8A_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HFH0180375.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
BASE COUNT         6901 a         1644 c         2941 g         5453 t
ORIGIN      
        1 ataattaatt cataaaataa aaatatgaaa ggatgatgtt gttcggtgat atttgatatc
       61 ataaaatcgt tgatataagt attgctggaa aagagtgaat aaaatcaaat ttaataagca
      121 aacaaagtaa agctatagtt ttattgttgt gcttaaaaat atttggttct attttactct
      181 tatttaaact catctttttg aagttaaacc acagagtaaa ctctgtggtt tttttatttt
      241 aaaaataaat tttaaggagt ggtatgtttt gttaaacaaa ttatgtatgt atttagaaag
      301 accagagctt tataaacaaa gtgaaattaa tttttgggat gatgagtata tttcagagca
      361 attattaaaa gcacacttag acactaattt tgaaggagca agtagaaatt ttaactttat
      421 cgaggattct gtaaattgga tagttacagt tgcaaatcca gttaattatc ctaaattact
      481 tgatttaggt tgtggtccag gtttatatgc agagaaattt gcacaaaaag gttataaggt
      541 aactggaatt gatttctcaa aacgttctat taactatgca caaaatagaa ataaggagac
      601 taatttaaat ataaattact tgtttcaaag ttatttaaat atgaactata atgaggaatt
      661 tgatttggct accttaattt attgtgatta tggagctttg tcaactgaaa atagaaggtt
      721 attaatggaa aaaatttatg atagtttaaa acctggagga aaattaatat tagatgtatt
      781 tacaataaat aaatacaata attttgagga aattaaatat tgggaaataa atgaagatgg
      841 aggattttgg agtaatgaaa aatatatgtg tctacaagat aattgtaaat acaatgatta
      901 taatacatta gaacaaactc ttgtaataac cgaaaaagat gaaaatgtat tttatgtttg
      961 gaatcactat ttttcaaaag aaagtttctt attagaagtt gaaaatattg gatttaagtc
     1021 tgttgagttt tttagtaatg ttaagggtga agcatattct gacgatagta tgactatggg
     1081 attagtttta caaaaataat aattttttta aaatgaataa ataaaaactt gaaaccttta
     1141 acaataaaat aacataagaa aagcttacat attgaaaaat ggaaactttt atgttatttt
     1201 tagtacatta caaatttgtt tatataaaaa tcaatacttg aattattatt tttatgtgaa
     1261 atttatctat agtatatttt tataaacaag taatgttatt aaaattaagt atatagttaa
     1321 aagtgtatag caaaaaggct gttatattaa tgttagaggt tcagggaaaa tatataaagc
     1381 aaagatattt acagataatg tagattgcac agctataagt tagataatag aattatgtaa
     1441 tcaagaattt acaacaaagt ataagataag gataatgcta aattgccata caggtgctag
     1501 atgtgtaaat ggatttaatt aatactattg ataaagtgca acaagcacat aaaccaattg
     1561 atgagattat tgaaactata agagatatag ttgacataat taaaatattg gaacctgttt
     1621 ataattttaa atcaaatcaa taatgattaa gattaaaata atattaaggc atcaatgtta
     1681 taagagtaca atataaggag tactatatag tactccttac ttatacatat taggcaatag
     1741 ggaatttttt tatagttaaa ttttcaaata ttttttaggg aactttataa ttgagaatta
     1801 agggtatagt atgtgatgaa cttactaaat ttataattag gttatataat ggaataatca
     1861 tcaatagttt tactttaggg aaaatatatg cttaaggaag tatagcttac taatattatt
     1921 aaaatgtata tttaaaatga taaaaaaaga attagacaag tatttagtaa attgactatg
     1981 caaaattcta tcaagcagga acactatgac tttagtcatg ggaggttcag taggtaactt
     2041 gttgaattaa gtttatatta tttaaataaa ctacttaatt tagataatat aaaaagtcag
     2101 aattttaaga atagagctat ataatcttag ctattaggag aagtactatg ataggaatat
     2161 attttagtgg aacaggaaat tcaagatact gtgtagaaaa atttttacaa gaatatgata
     2221 taacagcaaa ttcttattct attgaagata atgaactttt acagcacatc aataatcatg
     2281 agaatattat ttttagttat cctgtgcaat atagtaatgt gccaaaaata ttgaaagatt
     2341 ttataataaa tcattcaact ttgtggaatg gtaaacgtgt ttttattatt gcaactatgg
     2401 ctctatttag tggagatggc tctggtgtgt tagctagaat attaaaaaaa tacggtgcta
     2461 taactgttgg tggattacat gtgaagatgc cagatagtat aggagatgaa aaggtattaa
     2521 aacattcttt agagtataat agaaagcttg taataaaagc agaaaagaaa gtaactaaag
     2581 ctgctaaaaa actgaaacat gggtcaccac caaaagaagg gttgggtttt ttgagtcata
     2641 ttgtagggtt atttggacaa cgattgtatt ttatcaataa aacaaatatg tatacagata
     2701 aactaaaaat taatactgag gaatgtattg tatgtggtaa atgcgttaat ttatgtccaa
     2761 tgaagaatct agtaattaaa aattgtatga ttgttgcaaa tgggcaatgt acaatgtgtt
     2821 atcgttgtgt aaataattgt gccaaaaaag caattacatt gttaggtaaa aaagttgttg
     2881 aacagagtaa tattaaaaaa tatatgtaag aaatttaaag ttttattgaa tgtaaattga
     2941 tgtatatttt atgttagaaa gagaggttaa ataatggaat actacatagt tgattcattt
     3001 gcaactaaac tatttaaagg aaatcctgct ggagtttgtg ttcttgacag aagaatacct
     3061 ttagagttaa tgcagaaaat agcagaagaa aacaatttac cagaaacagc atttgtagtt
     3121 aaatgtaaag gaaattatga attacgatgg tttactccta aagcagaaat tgatttatgt
     3181 ggtcatgcaa ctttagcagc agcttatgta atatcaaatt ttattgatgt taatgtaaaa
     3241 aggatagatt tctttactca aagtggaaag cttgaagtta cacgaaatgg aaatctatat
     3301 gaaatgatat ttccagaaat aatgcctatt gaaatagagt tatcaccaca acaagctaat
     3361 ttgataggct gtgtaccaag tgctgtttat tcttcaagag atttaatctt attgctaaac
     3421 agtgaacaag aagttataaa ttataaacct aattacgcac aattgcgtaa attgacagac
     3481 tggttaggta taataatcac agcacaagga agtaatacag attttgtatc aagatatttt
     3541 tgtccagaat tagattcaga agaccctgtt actggttcat cacattgtaa tcttattcca
     3601 tactggtctg aaaagttggg aaaacacaaa atggtagctg ctcaactttc taatcgtggt
     3661 ggtataattc agtgtgaagt gttgaaggat aatactgtaa aaatttctgg tgaagcagtc
     3721 ttgtttatgc agggaacaat aaagattgat atttaaataa gtataataac taatttatta
     3781 tgaaaaataa ttaattttct tagtgttaac aataataaag tagagttaaa aagcttgatt
     3841 tcacttaaat caatatgata gatgttgata ctaagattga caagcatgat taaaaaagat
     3901 aatatttaat ctataaagat aaaaacacag tctggttggt agtccagacg cagcaatagc
     3961 tgtcagtaac ctgcctcctt ggttgtccgt tctttagaaa gtaatttaag gaggaactag
     4021 caatggacaa aattagtgga aaattaacag ttttatttga agaaccattt tgggtaggta
     4081 tatttgaaag acaagatggt aaaaaatatg aagcatgtag ggtagttttt ggagcagaac
     4141 ctaaagaagt agaagtgtat gaatttatac ttgaaagatt tttctcatta gattttggta
     4201 gcattaaatt agaaaaaaat gtgaccaaag acaacattgg atacaaaaga atgcagagaa
     4261 aagtaaaaaa ggaacaagaa aaagaaacta tagggactaa ggctcaaaat gctcttaagc
     4321 ttcaatatga agaaagaaaa caggatagaa aaaacctagc taaaaataga aaagaagaag
     4381 aaaaagaaag actatttaat ttgaaacaag aaaagagaaa agctaaacat aagggtcatt
     4441 aattttaatt aaatatcttt ttaataattg ctagaaaaag gtgtataaaa tttttaattt
     4501 tatacacctt aatttattga aaagaataag gttttatatt tcaattactt taaaatataa
     4561 aaatatacta ataaaaattg taaaaatagt ataaatggta taccaaatgt aaatttaatg
     4621 tgtttagttt tatgtctaaa agaatacatt ccaattatag aacctatact acctccaatt
     4681 actgctatag acataagagt agcttctttt attctccatt cgtttctaat tgctcttttc
     4741 ttatctatgt acattgaaaa gaaagcaata gaattaatca ctataaaata tattagaata
     4801 aaatttttca cagtcttgcc tccataaaaa tatattatac attataaaac aagatatttg
     4861 ttttggaaag ttaaatttgt aattagtaca tataaaatat ttgataagaa caaatataat
     4921 attaattgga aaatttaaaa aaattaaaca attagcaaat agggggtata tttataagac
     4981 acaaagtgat aaaatctatt tagaaaataa tgtaaaataa caggaggata aagcaaaatg
     5041 aaatttatag tagaaaaaga agtttttgat aaattagaaa atgtatgctt tggtgtagta
     5101 gttgcaaaag gaatagataa tactaaagaa atagaaagaa taagcaacct tttagatata
     5161 agtatagata gagtagaaga ttacttcaaa gataaaaaag taaaggaatc agaagaaatt
     5221 ataccatata gagaggcatt tagaagtcta ggcatgaacc caaataaatt tatgagttcg
     5281 atagaagcaa tgacaacaag ggttgctaag aataagaaac ttcctcatat aaatcctatt
     5341 gtagatttag gaaactctat ttctttaaaa tatttacttc caatgggtgc tcatgatatg
     5401 gattttagaa atgatgatgt atatgttaga ttttctaaaa aaggggataa atttgtacca
     5461 tttggagaaa ctgatgttga attaatggaa gaaggagaat taatttattc agttggagac
     5521 atggtaaaaa ctagaagatg gatatggaga caaggagagg aaggaaaaat tactaatagc
     5581 tcaaaaaata tattttttcc tatagatggt tttactgatg ctaatttaga taaggtaatg
     5641 agtgcaagag aagaattagc aaaattgtta aaggaaatat ttaattgtga aattaaagta
     5701 ggttttgtag ataaagataa tcctgaaatg gaaatataat aagttgttga atacttgtta
     5761 agatattata tttacttaat gatgttaaaa aggtattaaa aaagaaaggt cgtagtaaat
     5821 ttgaaaaaaa ctataataaa gaatgtaaat ccaatgattg aaaataagtt tataggactt
     5881 tttgaaatag agtataaaaa caaattagat gaagataagg tttggatggt agcatcaaga
     5941 aaaagtagtg agcaattaaa aaatatttat ttgaagaatg aagaagatag tgtagatgca
     6001 gttgtaatag ttgggcttca taaaagtagt aaaaaattaa ttttaataag acaatttaga
     6061 gtacctatta atggatatat atacgagtta ccagcaggtc ttgttgatga aggagagtct
     6121 atagatattt ccgttgaaag agaacttaga gaagaaacag gacttactct tttagaaatt
     6181 aaaaaaaata aaagtattga taaagtttat ttatctcctg gaatgagtga tgaatctgtt
     6241 gcctttgttt attgtatatg tgatggtgat ataacagatg aatttttaga accagatgaa
     6301 gagattgaag cgcttttaat ttcgcaagaa gatgcaaaag agattttaca aagtaatcat
     6361 aagatagaca caaaagcatt tttgatttta caaatgtttg tgagtttagg agctaaatta
     6421 tttgaataga tatatagaaa agaggttata tagaagagta aaataataaa gtttatttta
     6481 cttgtataaa ttataatctc tttttttata caataaagta taattttgtg tatttttaaa
     6541 taagtgactt gactcttaaa ttttcataat atgttataaa ccaatttaag atactacaat
     6601 aattaaaatt ccaaaattat ttaacatatt tgcaattaaa atgagaaaag gtaaaggttg
     6661 aatgattgaa aaatttgatg taaaatagta gcttagttta atattatagt ttgtagggtt
     6721 aaaaattaga aattgtaagg gagataaatt ctatgaaaaa attagttatt attttaatta
     6781 gtatcattag tatggttagc ttgacagctt gtaaaaaaag caatgaaagt aaatataata
     6841 atttaaacaa caaagaatcc ggacaaacaa ccattgaaat ggacttagat aaaaattatg
     6901 atacttcaga cccatttgtt aatgcacgtt tgttttgtgt gtctaatgat atagatgttt
     6961 tagacacaga gatttctttt aaaatggatg gagatagtgg tattgtggaa ataaaggaca
     7021 ataaaacata tgaaacttta tggagtaata catggcatgg aagcgttgat aatgatacat
     7081 tcacaatttc tctttctaat atacaaaaag agaaagagta tgctatatgc tttactggaa
     7141 ttaaaataaa tcatgcagtt gtaaaggtta cttttgaaag caatttagtt aaggagaaag
     7201 aaaaaccctc aaaataaatt ccagattgtc tataacttga tattcagtac ataaaagcag
     7261 ttgatattta tgattgactg ctttttctta tcaaaaagga gagtttgtgc atttttactt
     7321 gaaatagttt atgcacatca aaagttggtg agcatgatat tggaaatggt aagcatgaaa
     7381 tcttgataag acaaggaaat aaatttaagt tttcaattaa gtatatttcc ataatttagt
     7441 taacaataag ctaattatta tttatatttt gatatataaa taataattgt agcaaatttc
     7501 aaaaaatcat atattttttg aactaaataa aaatttattt ttattatttt aaattagagg
     7561 agattcttga agatatatta tttttaaaaa taagtatagt ttaaaaattc aaaaaaaaga
     7621 tggcaaaata tgtcccaagg gacattgagt aaaataaatg atttcagctt atttacacta
     7681 aaaagtacat actaaatgag gggtaaaata aagaaaaatg atatactaat tgtcttttga
     7741 taataaaaaa acatggaatt tgattattta atcaagaaaa agtattgaac aaatgattta
     7801 tttagtgtac aataatcatg taaaaacata gtaatggagg gagacatttc ataatgaaaa
     7861 ttaaggtagg aatgaacgga tttggaagaa taggaagagc tgtattaaga atagctcaag
     7921 aagaattagg agataatata gaaattgtag ccataaatgc aagagcaaca acagaatctt
     7981 tggcacattt atttacatat gattcatgct atggaacttt tagaggtgaa gtagaggcaa
     8041 aggatgaaga cactctagta gtaaactcta aagagataaa aatattaaga tataatgacc
     8101 cagaagaatt accatggaaa gaactggggg ttgatatagt aatagaatca acaggattat
     8161 ttacacaaag agaaaaggca gaaaagcata ttaaagcagg agcaaaaaaa gtaattataa
     8221 cagctccagg aaaaaatgaa gatataacta tagttatcgg agttaatgag aaagaatatg
     8281 ataatgaaaa gcacaatata atttcaaatg catcttgtac tacaaattgt ctagctcctt
     8341 ttgctaaagt attggacgaa aaatttggta tagtaaaagg gttaatgact acagtacatt
     8401 catatactaa cgatcaaaga atattagata aaagccataa agatttgaga agagcaagag
     8461 cagcggctga atctataata cctaccacta caggagcagc aaaagctgtg tcaagagtac
     8521 taccacaatt agaaggaaaa ttaaatggtt tttcacttag agtaccaaca ccaacagttt
     8581 cacttgttga tttagtgtgc gaacttaaag aaaacgttac atctgagcaa gttaactctg
     8641 tgttaaaaga tgctgctgaa ggagaattaa aaggtgtttt aggatattgt gacaagccac
     8701 ttgtttctat agattataaa ggagattcaa gatcatctat tatagacgct ctatccacaa
     8761 tggttataga agacaatatg gttaaggtag tttcttggta tgataatgaa tggggatatt
     8821 cttcaagaac tgttgactta gtaaaatatg tagctgaaaa attaaaataa tatatatttc
     8881 ttaaagcaaa ttttaaaaaa attttttaaa aaaattatat aaaactaaaa aaaaagatta
     8941 tgaaaaatat tgaaggaaaa attttttcat aatctttttt ctttcatgca aaaagataca
     9001 agttacaact tatatttgca tattaggtcg ttagagaagt atatatatat ttagtaagga
     9061 atcatacttt ttataaaatg aaagataaga tgtcgaaaac ttgcataaat ctgaaaaatt
     9121 tttattatta aaatactgat ttccatttta aaaatgataa aaaaatgaca aataaagttg
     9181 caaatagtaa tacttataag tataattaaa aaagagaaaa ggttttgtta aactttataa
     9241 caaaaaaaga agggatgatt caaaatggag aagttaacag acaggagaca ggttaaggca
     9301 gtaagcatgg aaaccttcat tttcctaata ttactagtag ttggttttgg gtatgttggt
     9361 agtattatgg gagcaggaat gatgttcaaa gtaataatga gtacagcaca tgctttacta
     9421 ttggagacag ttttcttaat tatggctatg gcagttcttg caggcgcatt aagcgcacta
     9481 ctttcagagt ttggagtaat agcgttaatt aataaaattt tcgctgtatt tatgaagcct
     9541 ttatatggac tacctggtgc tagtatagca ggagctataa caacttattt atcagataat
     9601 ccagctatta taccttttgc taaagataaa acattcacac agtattttaa acaataccaa
     9661 gttccagcat tatgtaactt aggaacagcc tttggtatgg gacttatctt aacaacattt
     9721 atgatttctc aaggtacaga atatgtactt ccagcattaa ttggtaattt aggtgcaatt
     9781 ataggtagtg tcataagtgt tagaataatg ttgacattta ctaagaagtt ttataaatac
     9841 agtccggaag aagataaagc aacaggaaca ttagagaaaa aggatgaatt tagagaaatc
     9901 agagaaggaa atgtatttca aagagcttta gatgcaattc ttgaaggtgg taaaatgggg
     9961 gtagatatgg gaatggctat aattcctgga gtcctagtag tatgtacatt ggtgatgtta
    10021 ttaacttttg gaccatctac agaccctgtt acaggtcaag aagtttatac aggagctgca
    10081 tatgaaggaa taaaattact tccagttatt ggagataaac ttggatttat attagaacca
    10141 ttatttggat ttacatcacc agaggcaata gcattcccta taacttcatt gggagctgtt
    10201 ggcgcagcaa tgtctctagt tccagagttt ataaagtcag gagctataac tcctaatgat
    10261 atagcagtat ttacagctat gggtatgtgt tggagtggat atttaagtac acatattggt
    10321 atgatggatg cattaaatgc tagacagttg gcaggtaaag ctattctttc tcatacaata
    10381 ggtggtttat gtgcaggagc agctgctcac tttatattta cactcgtagg ataaatgtaa
    10441 tcagctaaga taaaaaataa tatacaaaaa atggttgttg atatgtaata agaatttaca
    10501 tatcaacaac catttttttc tgactaaaga taatttataa tacataaaca ttaagtggat
    10561 attagagtta agttatattt atgtaaaaag taacaaaaat ctgtagaaat ttatgacaaa
    10621 atttagtaca ctaactatat gttcttattt aaaatttaag gttagaatta ggaattacct
    10681 agtagagtaa gctttgatta ataaaagaat cataaaataa aaatattagt cataaagatt
    10741 ataaataact tacttaaaat aaagccatag aacttacaaa aattaaaaaa gcatataaat
    10801 atggaggggt acaatggatg aattatttca tataggaatt gatgtaggtt ctacaacggt
    10861 gaaggttgtg gtacttaata atagtaataa tatagttcat aaagaatata gaagacatta
    10921 ttctgatgta aaaaaatcag ttaaagaagt actaaatggt atatatgaaa aattgggaga
    10981 tataaatact accattataa taactggttc aggtggaatt ggaatatcaa aaaaattagg
    11041 tgtaaaattt gttcaggaag ttatatctag tacaaaatca atagagtatt ttcatcctga
    11101 gactgatgtt gtcatagaac ttgggggtga agatgcaaag ataacatatc taagtggagg
    11161 aatagaccaa agaatgaatg gaatatgtgc aggtggcact ggagcattca tagaccagat
    11221 ggcatcactc ttaaagacag atgccagtgg attaaatgaa cttgctaaag ggtataatgt
    11281 tatatatccc atagcttcaa gatgtggtgt ttttgctaaa acagatattc aacctcttat
    11341 aaatgatgga gccaatcaga ctgatatagc aatgagtatt ttcaatgcag ttgtcgtaca
    11401 aactgtgagt gtcctttctt gtggaagaaa aatagaagga aatgttgcgt ttttgggtgg
    11461 tccattgtac tttttatctg aacttagaga agcatttaaa agagtattag atttaaaaga
    11521 tgaaaacata atcttcccag aaaatgcaca actttatatt gctattggag caggattatt
    11581 atcagtagat gaagataata tacaactaaa atctctaata gaaaaattgg atagtataaa
    11641 gaatattgaa gatggagaag taaatttatt agaaccttta tttaaagata aaaatgagta
    11701 tgaagaattt tcaagaagac atgaaaaaga aaaaataaat tatgtggaca taaattcaat
    11761 aaaaagcaat tgttatttag gaattgatgc aggctcaact acaactaaag cagcacttat
    11821 tgatgaggat ggaagacttg tatattctta ttataatagc aatgaaggaa atccattaaa
    11881 aacaaccatt aaagttataa atgaagtata tgatatatta ccaaaaaata ttaaaatatt
    11941 gagttcaact gtaactggat atggagaagg tcttattaaa aaagctctta aaatcgataa
    12001 tggagagata gagactatag cacattacaa agctgctaaa tttttcaaca aagatgtaga
    12061 ttttatactt gatattggtg gtcaagatat gaagtgccta aaaataaaag atggagtaat
    12121 tgataatata atattaaatg aggcttgttc atcaggttgt ggctcatttt tagagacatt
    12181 tgcaagttct ttatctatga caatagaaga atttgcgcat gaaggtattt attctcaaaa
    12241 tcctgtagat ttaggttcaa gatgtactgt atttatgaat tctagagtaa aacaagctca
    12301 aaaagaaggt gcaagtgttg gagatatatc agcagggcta tcttattcag taataaaaaa
    12361 tgctttattt aaagtaataa agattagaga tttaaatgag attggtaata atattgttgt
    12421 acaaggtgga accttttata atgatttggt gcttagaagc tttgaaaaac taattggaaa
    12481 aaatgtaatt agacctaata tttcaggaat tatgggagct tttggttcag cacttatagc
    12541 taaagaaaaa tatgaaagtg gatacaaaac atccttgtta tctaaggaag agttgaataa
    12601 tatcaagcta gaagcaagtg taactagatg taaaggttgt tcaaatcatt gtcttcttac
    12661 aataaataaa ttttctgata atgaaatttt tatttcaggt aatagatgtg agaagggtga
    12721 agctatttat ggtgataaaa aaataggatt agaaaagaaa aaaccaataa atttatttaa
    12781 gtacaaatac aatagaattt ttagatacaa accattggaa aaagaagaag caaaaaatgg
    12841 ggaaataggg ataccaagag tattaaatat gtatgaagat tatccatttt ggtttacttt
    12901 tttcaatgct atgggcttta gagttgtact ttcagatagg tcatcaaaac agttatatga
    12961 aactggtata actagtattg catctgaaac agtttgttat ccaggaaaat tagttcatgg
    13021 acatatagaa aatttaattg caaaaggtat aaaaaatata ttttatccaa gtgtaacaaa
    13081 tgaaaataaa gaggattcaa atgcagataa ttattataat tgtcctgttg taatatcata
    13141 ttctgaagta ataaaaaaca atgtagagaa tataagaaat aagaacataa attatataaa
    13201 tccatttata agtctaaatg ataaggaaaa attgaagaaa agactctatg atgaattatc
    13261 aagacatttt tctgatataa aaataactaa agaagaagtt aatcatgcag ttgataaagc
    13321 tactgatgaa cagaatttat ttaaaatgga gatacaagca gcaggtgaaa gagcacttaa
    13381 agaaataaaa gaaagaaata tgaaggggat tgtgttatgt ggaagaccat atcatataga
    13441 cccagaaata aatcatggaa tgccagagct tataaattca ttagatatgg cagtgcttac
    13501 tgaagactct atatgtcatt tagccaatgt acaaagacca cttagagttg tagaccaatg
    13561 ggtatatcat tcaagattgt ataaagcagc tagttttgta agagataaaa actatttgga
    13621 acttgttcaa cttaattcat ttggctgtgg attagatgca gtaactacag accaggttca
    13681 agaaatactt aatgaaaaat cgaagatata tactattata aagattgacg aaggcaacaa
    13741 tttaggagca gctaagataa ggatgaggtc cttaaaagca gctatgtttg agagagaaag
    13801 caaaaatata gatgtaaaaa atataaaggt aaacaggata cagtatgcta aaaataatca
    13861 gataacgaac aaacatacta tattagcacc acaaatgtct ccaatacatt tccaattcct
    13921 tgaaaaagct gttaacttaa gtggttataa tatagaaatt ctaaaagata ctgatagtag
    13981 tgtaattgaa gaagggttaa gatatgtaaa taatgatgca tgttatccag ctataatagt
    14041 tgtaggtcag ctaataacag cattaaaatc tggcaaatat gatttaaata acacttctgt
    14101 tactataact cagactggtg gaggttgtag agctacaaat tatattggat tcttaagaaa
    14161 ggctatatac gatgctggat ttaaagatgt tcctgtcatt gcacttagtg taaatggaat
    14221 agaagatagt ggtataatgg ataatatatc actaaaatta atcaatagac tctttatgtc
    14281 tgttgtttat ggtgatttgc tgatgaaagt actatataga gttagaccat atgaaaaagt
    14341 tactggaagt gcaaatgctc tatatgaaaa atgggttgat atatgcaaat cttctcttgt
    14401 taaagcaaaa atttctgtat ttactagaaa tataaaagat atagtaaaag aatttgataa
    14461 tttagagata ctagatatca aaaaaccaaa agtaggtttg gttggagaaa tattggtgaa
    14521 attccatcca attgcaaata ataatttagt agatatatta gagcgagaag gtgctgaagc
    14581 agtcgtgcca gaccttacaa atttcttctt aagttgtgcc tttaatacta tatacaaaca
    14641 cactcattta gaaggaagta gaaagagtag aatgatagga gaggcattta tatacataac
    14701 tggaatatat caaagagtat ataaaaaagc acttgataag agtgagagat tttatgctcc
    14761 agccaatata aaaaatgttg ctaaaagtac agaaccagta gtgtcattag gtaatcaaac
    14821 tggagaaggt tggcttttaa caggtgaaat ggttgaatta ttaaatgaag gtgtagaaaa
    14881 cattatttgt atgcaacctt ttggatgttt acctaatcac ataattggta agggttctat
    14941 aaaagaatta aagaggttgt ataaaaatgc aaatataata cctattgatt atgacccttc
    15001 tgcaagtgag gtaaatcaac ttaacaggat aaaacttatg ctttctaagg cttttaaaaa
    15061 tatataagat ttatattttg atatgttaaa agttaatgaa tttaaaaata tactctatat
    15121 taagtatcta taaaaaagag ttatcctatt ttttagggta actctttatt agttaaaaaa
    15181 ttatatgtat actgatatta aaggggggaa gtaatatgag tttagtatat gatgtttata
    15241 aatcaaaatt aggaaatttg tacatattaa gtgaagatgg ttttatagtt agtatataca
    15301 taggagatga aaaatttaat aatttaaaaa atgaatgtat taaaaaagga attgatataa
    15361 agagaagtaa ggaagattta aaggaagcaa taagccagat agatgattac tttaatggaa
    15421 aaagaaaagt ctttaattta aatattcgta ttgaagggac tgaatttcaa aaatcagttt
    15481 atagggaaat gttaaaaata ccttatggag aaacattgtg ttattcagat atagcaagaa
    15541 atataaacaa tccaaaatca gtaagagcaa taggacaagc aagtaaaagt aataaaattc
    15601 caataataat accttgtcat agagtagttg gcaaaaaaga tattggtgga tatatgggga
    15661 atcattctga tttgaaagag atactattaa atttagaaag acagagttaa cttagaaaga
    15721 aatgttttta ttgttaatat ttgatataat aatagtaaaa agaagtattt tttacaaaaa
    15781 caatgaagaa tttattttaa tttacaaaaa tgagggggaa ttttaatgtg tgataaagtt
    15841 atagcatcaa aatatcatga gaagggattt aattgtgcag aatcagttat aaaagcatat
    15901 aatgaggaat ttaatacaga tataccagtt tgtttaggaa gtggactggg ttcagggtgt
    15961 ggggttgcta gtttatgtgg tgcagtaaat gcatcaaata taataattgg atatgtaaaa
    16021 ggcagaaacc acgaagatga aagtactaaa gctaaagtat atgctaaaga tttaactaca
    16081 acagtaagaa aagagtatgg ttcagagtta tgtatagacc taaaaaaaga tttagttgct
    16141 tgtagagaaa taatggattt cgcatatgat agtctaaagg aaactttaaa aaaagaatta
    16201 taagaaaata agctatcaaa tataaattat ttgatagctt atttttatag ccgatactaa
    16261 ataaatcaaa tatattttag aattattatt tgtaaataaa cagcaaataa ttttaggctt
    16321 tttatttaat atagttttta tttatattta tttttgaata tgttatactt tgtttaaata
    16381 aatatttgta atgagggaga tgagaagatt gaagaatctc aatttactta ttaataaatt
    16441 atattcaaaa aatcataatg aagcatataa aactttttta ttcttagaaa atgaaagttt
    16501 aaaatcaaat attacatatt gtttttttga ctcattccta gaaatgattg ataacgaaaa
    16561 ctcatatatt cgaacaaggg gattgttatt aatctctgca aatgctcagt gggatattga
    16621 taataaaatt gaaataaata tagatagtat actaagtcat atcgtagata aaaaaccttc
    16681 tgtatcaaga atgtttatta aatccatacc taatattaca aagtataaag aaaatcttat
    16741 taatcgcata aaaatggaat taagtaatgc agatataagt atttataata ataatatgaa
    16801 accattagta gaaaaggata taaacgatac attatctaat attagttaaa tattaatata
    16861 gaaattgaat attatctagt tacaattatt tataactaag ataataaaac aacaatggtg
    16921 ctaatttata tgtataaat
//
LOCUS       DAYATN010000078        16626 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.078, whole genome shotgun sequence.
ACCESSION   DAYATN010000078 DAYATN010000000
VERSION     DAYATN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.078"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            172..840
                     /locus_tag="ACGI8A_002381"
     CDS             172..840
                     /locus_tag="ACGI8A_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFH0180376.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            842..1327
                     /locus_tag="ACGI8A_002382"
     CDS             842..1327
                     /locus_tag="ACGI8A_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFH0180377.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1388..2509
                     /locus_tag="ACGI8A_002383"
     CDS             1388..2509
                     /locus_tag="ACGI8A_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HFH0180378.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2790..3980
                     /locus_tag="ACGI8A_002384"
     CDS             2790..3980
                     /locus_tag="ACGI8A_002384"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFH0180379.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEISRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4114..4369
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4428..5429
                     /gene="asnA"
                     /locus_tag="ACGI8A_002385"
     CDS             4428..5429
                     /gene="asnA"
                     /locus_tag="ACGI8A_002385"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFH0180380.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5533..5922
                     /locus_tag="ACGI8A_002386"
     CDS             5533..5922
                     /locus_tag="ACGI8A_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HFH0180381.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            6083..7291
                     /locus_tag="ACGI8A_002387"
     CDS             6083..7291
                     /locus_tag="ACGI8A_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFH0180382.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7377..8453)
                     /locus_tag="ACGI8A_002388"
     CDS             complement(7377..8453)
                     /locus_tag="ACGI8A_002388"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFH0180383.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8682..9209
                     /gene="hpt"
                     /locus_tag="ACGI8A_002389"
     CDS             8682..9209
                     /gene="hpt"
                     /locus_tag="ACGI8A_002389"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFH0180384.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9298..10230
                     /locus_tag="ACGI8A_002390"
     CDS             9298..10230
                     /locus_tag="ACGI8A_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFH0180385.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10292..11518
                     /locus_tag="ACGI8A_002391"
     CDS             10292..11518
                     /locus_tag="ACGI8A_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFH0180386.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11602..11823
                     /locus_tag="ACGI8A_002392"
     CDS             11602..11823
                     /locus_tag="ACGI8A_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFH0180387.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            12012..12182
                     /locus_tag="ACGI8A_002393"
     CDS             12012..12182
                     /locus_tag="ACGI8A_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180388.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12275..12736)
                     /locus_tag="ACGI8A_002394"
     CDS             complement(12275..12736)
                     /locus_tag="ACGI8A_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180389.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKV"
     gene            12926..13387
                     /locus_tag="ACGI8A_002395"
     CDS             12926..13387
                     /locus_tag="ACGI8A_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180390.1"
                     /translation="MLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKIDS
                     VFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKNNI
                     DISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            13430..15193
                     /locus_tag="ACGI8A_002396"
     CDS             13430..15193
                     /locus_tag="ACGI8A_002396"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HFH0180391.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            15201..15809
                     /locus_tag="ACGI8A_002397"
     CDS             15201..15809
                     /locus_tag="ACGI8A_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0180392.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            15881..16450
                     /gene="pduL"
                     /locus_tag="ACGI8A_002398"
     CDS             15881..16450
                     /gene="pduL"
                     /locus_tag="ACGI8A_002398"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFH0180393.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         6501 a         1685 c         2936 g         5504 t
ORIGIN      
        1 atattatatt tttaaacttg aaaagtaagt aattataagc tatttaatca ctggaataaa
       61 tttacaataa aaaaataaat acaaaggctt tatcattaag gtattggcat aagttttgct
      121 tattaaacaa gtatactaat aaaattcatt taaacaggag ggagaattta tatgagtaaa
      181 ttagaatcaa caacagaaaa agttgaggta aaatggtatg ggtatgtagc attaatattg
      241 ggagcattat ttttttcagg aatatttaaa gatgcaccag gaccattaaa agtattggat
      301 tttaacaatg tattaggttc ttttggaaca ttgggaacaa ttaatgatgg tgtaggtaca
      361 ttagctgcaa actttagagg tgatggaggt actggccctc gagatggatg gctatatgca
      421 ttgacattaa taccatcagt tatgtttgca cttggagtag ttagagttat agaccactta
      481 gatggaatga aggcagctca aaagcttctt agtccactat taaagccatt attagggtta
      541 cctggatttg caggactaac tttgatagct agtttacagt ctacagatgc agctgcatct
      601 atgactaaag aacttaaaga tgatgggtat atagatgaaa aacaaaaagc tgtattttgt
      661 gcatttcaat tttcaggtgc gtctgcaatt acgaatttct ttgcttctgg agcagcacta
      721 tttccattta taggagatgt accaatattt ataccacttg ctttaatatt aattatgaag
      781 tttgttgggg cgaacttact tagattatat ttaaataaat ttgacaaggg ggaagcttaa
      841 catgagtgag gtagcaaaaa aagaagtaaa agttaaaaaa ggaaatgtga tagatgcgtt
      901 tattggagga gctagaaatg gattccaaat aagtacaaat tcaatggctc caaatgtcat
      961 cttcggtttt gctataattt ctgtgttcaa cttaacaggt cttttggatg taatagggac
     1021 aatatttaca cctgtaatga gtatctttgg acttccaggt gttgcagcaa cagctataat
     1081 gacaatattt atatcaatgg gaggagcatc tggagtaatc gcaggtttat ttacagcagg
     1141 tcaaataagt caagcacatg ttgcaattct attgccaatg ttattgcttt caggagcgca
     1201 gatacaattt atggggagac ttttaggaac agctgagtta aaaactaaat attatccaca
     1261 tatgtttgtt atagcaactt taaatggttg tattgcaatg cttataatga attatttcgt
     1321 agtataggta attataataa aaatttaata taaatggaaa gtttaaattt taaggaggag
     1381 ctagattatg ggaactaaca aagattatat tgagtatgta cataaagtgt atgattatct
     1441 acacactata ccagaaaaag gatttgaaga atataagaca tcagagtttg ttttaaaaga
     1501 gcttgaaaaa atggggtttg aaaatattaa taaaggagtt gcaggaactg gtattatagt
     1561 aactatagat tctaaaaaag aaggaccagt atttgctata agagcagata tggatgcatt
     1621 ggaatttatc atagatggag aaaagaaaat gatacatgct tgtggtcatg atggtcatac
     1681 ttctatgctt ttaacagctg caaagttaat aaaagagaga aatttagtaa aaagaggtaa
     1741 gttaaaaata ctttttcaac caggtgaaga aaaattatat ggagcattaa gagtaattga
     1801 ttctggtttg ttggatgatg tagaagaaat ggtagggatg catttaagac ctggacaaga
     1861 ttgtaggttg ggaaaggcga tacctgcaat gtaccattgt gctagctgta taatggaatt
     1921 tacgattaaa ggtatcactt gtcatggttc taaaccacat ttaggagtaa atgcagtaga
     1981 tgctgtctgt ttagcagtga atgcaataaa tgcaataaga gtaaatccag ctataccaaa
     2041 ttctataaaa gtaacaaata tatcagttaa gggagaaact tataataata taccagatga
     2101 agcatttatg gctttagata taagggcaga aagtaatgat ataatagaag aattaaaaga
     2161 aaaagcaata catgctgtaa aaactgttgt aagttctata aatgcagaag ctgtactaat
     2221 ttccaaagat ggagtaccag cagcagaatt tgatgaagaa cttgtaaact tggctgaaga
     2281 aactataaaa gatgtattgg gagctgatgc atctataggt atatttaaaa attctggtgg
     2341 agaagacttc cattattata cacaaaaact aaaatgtaaa actacatata ttggattagg
     2401 agctgatgct acaccaggat ttcataatcc aaatgtaaca tttgatacga aagctttaga
     2461 atatggtgta gatatatggt gtagattggt tgaaaagaga ttgggataac atccaaacta
     2521 ataattctac atttaaaatt ataattatag aagttataga aaaagattgc ttatgttaat
     2581 tagaaaataa ttaatatgag caatcttatt ttgatataat gaaactgatt ttggaatatt
     2641 gaaaaaatat gatggaaaag taattatatt ttaaatctat tggacaaaaa agtgatatta
     2701 ttataatatt aagaattgta aaataatacc aaagtgcatc tagtaataga cttaacaatt
     2761 ataaaaattt tattcttagg agggaaaaaa tgagtaagta taattttgac agagtaatag
     2821 atagagtagg aacagattgt gtaaaatggg attttagaac taattgttca acaaaagcac
     2881 aaaaagatgg tttgcctttt tggattgctg atatggattt tgaatgtgca gaaccaataa
     2941 tagaagcatt acgtaaaaaa gttgaacata aaatatttgg atatagttca aatgatagtg
     3001 ataaatactt taatgcagtt tgtgattggt ataaaaggag atttaactgg gaaataagta
     3061 gaaaagatat tatttttagt cctggtgttg tacctgctgt tgctatttta gtaagaatat
     3121 taactaattc taatgaaggt gtaatagtcc aaaaaccagt gtattaccca tttgaagcta
     3181 agataaagag caataatagg gacgttgtaa acaatcctct gatatatgag aatggtactt
     3241 ataggatgga ttatgatgat ttagaagaaa aagctaagtg tagcaacaat aaagtactaa
     3301 tactttgtag tcctcacaac cctgttggaa gagtttggag agaagatgaa ttaaaaaagg
     3361 ttgtagaaat atgtaaaaaa tatgacctat ggataatagc tgatgagata cattctgatt
     3421 taattagaaa aggatttaaa catactcctt tacagtcatt atgcccagag tacaaggata
     3481 aaatagttac atgtatagct ccaagtaaaa catttaatct ggcaggaatg cagttgtcta
     3541 atataatagt gaataatgat gaactaaaaa agaaatatca agaagaggtt acagctgttg
     3601 gagttgcaac ttcaccaaat ccctttgcta ttgtagctac aatagcagca tataatgaaa
     3661 gtgaagactg gcttaatgaa ttaaatgatt acttagataa taatatacaa tttattgatg
     3721 aatacttaaa agaaaaccta ccaaaagtaa aacttgttta tccagaagga acatatctag
     3781 catggttaga ttttactgct tatggattaa atgaagtaga acttgaagat ttaatgttta
     3841 aaaaagctaa tgtattattt gatgaaggtt atatatttgg aaaagaagga ataggatttg
     3901 aaagaataaa tgtagcttgt ccacagtctt tattaaaaga atgtatggac agattaaaga
     3961 ctatatttga aaatttataa agtataggta aaaatttata aagtgtttaa aaataaaaaa
     4021 ataataaact tgacaaattt taactaatca cttatcatac tattatgaat aaatagaaaa
     4081 cataagagag ctaaatttta ttgaattaaa ctcttaaaga ctttgaaagg aagagtagtt
     4141 atagaatgta attaaagcga gttggtgatg gtgtgagacc aatatgaagt ctatagtgaa
     4201 gaacatcctg gagttgctaa ccgaaatttt aaaagagtag gcttagtcgg aaccaaaccg
     4261 ttatcatatt ggggtgtatc gagtaatatc tgtacattgt gagctgggtt tgaatttatt
     4321 tcaaactaat cagggtggta ccgcggaaaa gcaacctttc gtccctttta tatagggatg
     4381 aagggttttt ttaatggaaa aatatataaa tatagggagt gaatgatatg agcacaataa
     4441 taccaaagga ttataaaagt agtttgaacg taattgatac acaaatagct ataaaaaaat
     4501 taaaagattt ttttgaaatg agactaagta atgaattaaa tcttataaga gtttcttctc
     4561 cgttatttgt attgccagaa acaggtgcaa atgataattt aaatggagaa aaggctgtaa
     4621 gctttgatat accatttatg aataaaaatg cagaaattgt tcaatcactt gcaaaatgga
     4681 aaagattagc ccttaaaaaa tatgggtttg aagttggtag tggtttatat acagatatga
     4741 atgcaataag aaaaaatgaa gaattagaca atatacattc tctttatgta gaccaatggg
     4801 attgggaatt ggttatagat aaaagtagta gaaatgaaaa gactctaaaa gatgttgtaa
     4861 aaagattgta tggagtattt aaagatactg aaatttttgt atgtagtatg tatgaaggta
     4921 taaaagaaat attaccagaa gaaataactt ttataacatc acaagagtta gaagatatgt
     4981 atccagaatt atcaccaaag gaaagagaag ataaaattgt aaaagagaag aaagcagtat
     5041 ttataactca aataggaaaa acattaatat caggagaaaa acatgatgga agaagtcctg
     5101 attatgatga ttgggaattg aatggggata tattatttta caatcctgtt ttagattcag
     5161 cattagaatt atcttcaatg ggaattagag ttgatgaaga atctcttgat agacaattga
     5221 aacttgctgg atgtgaagag agaaaagagt ttgactatca taagatgctt ttaaatggcg
     5281 aacttccgta tacaattggt ggaggaattg gtcagtcaag aatatgtatg tatttcttac
     5341 aaaaagctca tataggagaa gtacaagttg gagtatggcc acaggatatg attcaaaatt
     5401 gtagtagtgc aggaattgaa ttattataat ttaaatataa actaattgat aaaatttata
     5461 tctaatatgt tcatcaaatt aattatatcg gttataatgt agaataacct ataattataa
     5521 gaggtgataa atatggagtt tttaaaaata atcattaata tagttttaga tattttaaaa
     5581 aaaattttgg tcagatttaa aaatgctaaa tttggtttgg tttttgtatt tgatttactt
     5641 aaactacctg attttatgac agataaaaga atcaatatag ttgataaaat aaaggtaatt
     5701 tctgtattaa tttttactat atcctacttt gtatctgggg ttgatataat tccagagatg
     5761 attgcagggg catttggttt tatagatgat gctatagttt taatttggag tataggtata
     5821 gtgaatgaag aaattaataa gtatagagtt atcgcaaaaa aagataaaca ttcaaatata
     5881 attgaaaatg tagaattttc tataaaagat gaagaagaat aacaatttat atatgttatt
     5941 ctttttttta ttaacaaaaa atggttaaat tgttgacgga gaggtaaaac tctattatca
     6001 taataagtgg atatccaata tttaactaag tattcaatgt gcaagaatgc ttaatttaaa
     6061 aaagttatgg agggtaaata ttatgaagaa aaaaataggt ttagtgccca aactaatcat
     6121 cggtatcata gtcggtattt taatcggaag ttatgcacca gaaataatag tacaaatatt
     6181 agtaacagtt agtactttat tctcagcatt cctaaaattt gttataccat ttataattat
     6241 aggttttgta acagcaggta tagctgattt agcaacaggt gcaggaaagt tattggggat
     6301 aactacggga atagcatatg gttctacctt aatagcagga ttattatcat ttgcagtatc
     6361 tacattaatc ttcccaaatt ttattgatgc aagtgttgca agtcaaattg gagacccaga
     6421 ggcaggaatg ttagctccaa tattcacaat tccattagag cctatggtag atgtaacagc
     6481 tgcaatcgta tttgcatttg taatgggact tggaatatca gctttaagaa atcatggaaa
     6541 aggagagact ttatttaatt tcttccaaga atttcaagga atcgtaacga aaacgttgtc
     6601 aacagtgatt atacctttat tacctttata catagcagga acttttgcaa atataactat
     6661 agcaggggaa gtatggacaa tattaggggt attctggaaa gtatacttag tagtaatacc
     6721 tttacatttt gtatatatgg catgtcaatt cacagcagca ggtgtatttt caggaaaaaa
     6781 tccagtaaga atgttaaaaa atcaagtacc tggatattta acagctatag gaactcaatc
     6841 ttcagcagca actataccag ttaatgttgg ttgtgcagaa aaaaatggag taagtaagca
     6901 aataagagaa tttgttgtac ctctttgtgc aacaatacat ttatcaggta gtatgatatc
     6961 aataacagga tttacaactg cagttttaat gatgaatggt atgccacatg gaataggtgt
     7021 attattccct tatattgcaa tgttaggtat agcaatggtt gcagcaccag gagcaccagg
     7081 tggggctata atgagtgcac ttcctttcct accaatggtt ggaataccat cagatggtgg
     7141 gcttgcaagt ttaatgatag ctttatactt aactcaagac agttttggta cagcatgtaa
     7201 tgtatcagga gataatgcaa tagcagtagt agtagataaa ataaatgatg gaatgagtaa
     7261 aaaaggaaat aaagaagagc aaagtgctta gttttttaat aatcaaaaat gagttatctc
     7321 aaatagagat agctcatttt ttatggtata aaaaattata aaataaagtg atttttttat
     7381 ttaaaaattg ctttaagaga tgatattaat aatttatttt cattaggttt taatatacat
     7441 actctaaaga aatactcatc tagaccttca aatgaacaac aatctcttat tattattttt
     7501 tgtggaagca gttgctctcg aagagattta gcagtcaatt cctttgtctt gattttacac
     7561 aatatgaaat tacctttagt attgtatata tctaagcttt ttatattctt aagctcttta
     7621 agcaggtaat ctctttcagt gttcattaag gaaatagtat ttgatataaa atctaaatca
     7681 ctaaacatga tttcacccat tttagaagct attatgttta tattccataa atctagattt
     7741 ttatttattt catttttgac attagtgtta gatataagac catatccaag ccttattcca
     7801 ggcgttgaaa aaaattttga tgtacctctt ataacgaata aattagaata atcatcaact
     7861 aattgagtac aagagtatgt atctgtatca gtaaactcta cataagtctc atcaatcatt
     7921 aaaaatgagt ctgtattttt taaaatttct ctcacttcta ctttagtaaa tgcaaatcca
     7981 gttggattac taggattaca gattactacc aaatcataat ctttagcatt gatagttttt
     8041 ataagatttt ctaaatttat atgaaaatta tcttcttctt ttgcaaagta tttttctata
     8101 gaacaattaa ttttagataa ctctttttca tactctgaat aggctggaga gagtaaaaga
     8161 gcctgtttag ggttaattgt atgtataaat gatgaaatta gttctgtagc tccactgcca
     8221 agtactatat tatctataga acatttgcag taattggata tagaagtttt taagtctatg
     8281 taatcagggt caggatacat agataccata tctatattgt ttacaatgta ttgtttggct
     8341 aaacttgatg taccaaaagg atttatattt gagctaaaat ccataaattc ttcctttgaa
     8401 aagccatact tactagataa actatataaa ttagctccat ggtttgtgct cataaaagta
     8461 cctccaaaaa taaaattata taatcgataa aaataactga ttaatatatg tacatataga
     8521 ttataataca aaggaatcgt tattccaaat ttttaatgta aaaagcaaaa aagtatggat
     8581 aagctgttaa tttaggttta aaaatgatga aaaagatttt aattgatatt ttggtatgct
     8641 ataatatata agttaataga aggaaagcgg gaggatacaa aatgtacaaa gttacaggaa
     8701 aaatgttgac agaagaacaa ataaaagaaa aagtttatga attaggaaaa aagatagaag
     8761 aagactttaa aggcgaagat ttactagtgg taggtatact taaaggagct agtgtatttg
     8821 tttcagattt aataagatgt atagatttgg atgtaaatat agattttatg agtgttacaa
     8881 gttatggaaa ctcaacagag tcttctggaa cagttaaaat attaaaagat ttagatgtag
     8941 atattgaagg aaagaatgtg ttgattgtag aggatataat agattctggt ttaacattaa
     9001 gtaatctagt tgcagcttta aaaactagaa atccaaaatc tttaaaacta tgtactctac
     9061 ttgataaacc tcagagaaga aaagctaata tacctgtaga ctatgttgga tttgttatag
     9121 aagacaaatt tattgttggt tatggaatag actatgctga aaagtataga aatttacctt
     9181 atataggtat agttgaggat gtagaataga tttaaatgtg aatcaaaagc actaatttct
     9241 aagggagtta gtgcttttta gttaattata taaaatttta gtaaaggaga catctatatg
     9301 gatattcagt taaatatatt attatttttc caaaatttaa gaaatccaat tttgaacttt
     9361 atatttcttg tctttacaat aagcacagaa gcaccgttat taatattaat tacaacaatt
     9421 atatactggt gtataaataa aaaatgtgga cagaaaatac tgtttgctat aattggaaat
     9481 tttgttgtaa atttaggcat caaagagttt gtaaaagctc ccagaccaat aggaataaaa
     9541 ggtcttgaat cattgagagt ttctactgct ggtggttatt cttttccaag tgctcatact
     9601 caaactgcaa caacattttg ggttagtatt atgactatat ttaaaaagag atatcttcat
     9661 ttaataggtg cagttatggt tttaggagta ggattgtcta ggctgtattt agctgttcat
     9721 tggccaatag atgtgatagc gggttggata ttaggaatat tttttacagt tgtatttgta
     9781 aaaatatttg actatattga tgataataaa aattatattt tacttgtagt tttacttata
     9841 ccatttataa tagtggctat atttctaaat agccctgaat attttgaaaa atttggtata
     9901 atagtaggat ttgtatttgg atatatggtt gaggatagat ttgttaaatt taatacagat
     9961 aataataaca aaaaaataaa ttttagcaat aaaaataagg ggagtaaaaa tataattttt
    10021 tcaagaatat gtaggttttt ggttgggata gtaacaatag gaatgttata tattggcatt
    10081 aagttgttta tttcaatgtt aatagtcact ttaaatataa gtgactctac tatgagtata
    10141 atttttatga attttattaa gaatacagta gttgtttttt atggaatagc aggagctcca
    10201 gcattattta aacttttaaa attaaattga ttttaaggta tagagtcaat agtttaaatt
    10261 aaaaaaatta agggtagaaa agagtgatta tttgaagaag gtagtaataa taggtggtgg
    10321 accagcaggg atgatagccg catcaactgc atgtgagaaa ggctatgatg taacattaat
    10381 tgagaaaaat cataaattag gaaaaaaact tgcaataaca ggaaaaggta gatgtaatat
    10441 tactaacgct tgtgaaatag aggaattaat agaaaatgta cctacaaatg gaaaatttct
    10501 ttatagtgct ttttatactt ttacaaatga tgatgtaata agtatgttta ataatcttgg
    10561 agtaaagact aaaacagaaa gaggaaaaag ggtttttcca gagagtgata aagcatttga
    10621 tattgtaaat gctcttgaaa gacaacttaa aagtaagaaa gtaaatatat tattaaattc
    10681 gaaagtagaa aaaataatat ctaaaaataa taaaattgaa aaagtaattt taaacgataa
    10741 aaaagaaatt aagtgtgatt cagttgtagt agcaactggt ggattaagtt atccacttac
    10801 tggttctact ggtgatggat ataaatttgc aatttcacag gggcacacaa ttatagatac
    10861 aaaaccatct ttaattggta tagaagtaca agaaagtttt acaaaagatt tagaaaaatt
    10921 atcattgaga aatgttgaaa taagggtgtt taatagcaaa caaaagaaag tttatagtga
    10981 ttttggagaa cttgaattta ctaaatttgg tttagatgga cctataatca agtctgcaag
    11041 ttgtagaatg aaagatacaa ggaaagaaaa ttacactata ttattagatt taaaacctgc
    11101 attagatgag gaaaaattgg ataaaagagt tcaaaaagat tttcagaagt atacaaataa
    11161 aaaatttgaa aaagcattag atgatttact tccaaaaaaa ctaataccta taataataaa
    11221 cttaagtgaa attaatccaa atacggttgt tcaccaaatt tctagagagc agagaaaaaa
    11281 cttagtccat ttattaaaaa atttaaagtt tacagtaaag agatacagac ctattgaaga
    11341 agctataata acttctggtg gtgtaaaagt aaacgaaata aattcaagta caatggaatc
    11401 aaaattagta gaaggtttat tttttgctgg agaagtaata gatatagatg catatacagg
    11461 tggattcaat ttgcaaatag ctttttctac tggatatttg gcaggtttta attgctaaaa
    11521 attacctgaa ttaaaaatgt ttaaaatcac ataatattat cagcaacaca aaataaatta
    11581 aacagaagga aggtaatcaa catggcaagt aacaataaca acaacagaac agtagtacca
    11641 ggagcaaaag aggctttaaa ccaaatgaaa ttagaaatag ctaatgaact tggtatgagt
    11701 aattatcaac aagttgataa aggaaacctt actgcaagag aaaatggtta tgtaggtgga
    11761 tacatgacta aaaaattagt tgaaatggct gaacaacaaa tggctggaaa aagcaacaga
    11821 tagttataaa taattattta taactattgg ctattagttt agctagagct atctcaaata
    11881 tagaaagtaa tttctaaatt gagatagctt ttttttatta aattattaaa tagatatatt
    11941 tttatgtaat aaattttatg tatttggata aaataataca aatgaatatg ttacataagg
    12001 agtgatgtaa aatgagtagt gtaaattttg aggatttaaa aaacaaattt attaatagtg
    12061 acttagatga aaaaataaga atatatacaa caacagaagg tttatcagta gaacaattta
    12121 aagagctact aaaatattat ccaatacaac atttatctaa actagaaaaa gcacttggat
    12181 agcagtattt aatccaaaat taaaaaggca atttatttaa tattaaaaat attttttatt
    12241 aaataaattg cctttttata ataggtttaa attattatac ttttgatata taattgtaga
    12301 ttctctcatt tccaagttca atagacccag tattaataag agtaactatt ccttctatat
    12361 ctctagcagt taagtattta tatggaatta ataaattata accagtatta cttagtaagt
    12421 tttttaattg ggttatttga gagatattga aacattttgc agttttagta gatttttttc
    12481 ttaagttaga taaattttca ttttgaatat ttattaaagt ttctatatag tttctataag
    12541 atatcttaaa gtttgtctca ttgactattt tttcattaag tatactactg ataaaatctt
    12601 gagacaatat attatctgca aatttatctg ctgttaattt tccagatgtt atgccttttt
    12661 gatattttct aagaattttt ttagtatagc agtatatttt atttctcgtt tctaaatcga
    12721 gttgttctat ttgcacaaaa ccacttcctt ctaaattata aaaatattta ttaaaaatct
    12781 atacttaact aatcaagctt caataactta tttacaataa atactaaata ataaaatatt
    12841 tgttattttt tattattacc aagtataaaa aatattataa ttgtaaaact tacaaatata
    12901 tgtaaaatat ttaggagtga tgaaaatgtt aaatgggata ataaagcttt tttattctga
    12961 aattgtaaaa acaaagatta ataacttaga ttcaagtgga tattctttaa gagatgaatg
    13021 taaagaaatc agaaatataa ctctaaagat agatagtgta ttttttaagc ttttaaagtt
    13081 agcttttggt tcaatattgc ttatggtagc ttttaattta aacatggttt taggtatagg
    13141 agtgttttta gttggtgtag tttatatact atataaaaga aatatggaag aacaagtgaa
    13201 agagcatata aataatgtga aaaataatat cgacatctct aaaataactt ttatggatga
    13261 aaaagggaga acaggtctaa atgcattaat tacattactt cttataggat tattgacagg
    13321 atttaactgg atattagttg ctagttttgt cgttgttttt gtttttacta taaagaatgt
    13381 atgttaaaat actaataaat agtttaatat atagggggag aaattgagta tgactaatgg
    13441 cagttataaa atgaagaatt tagtggaaat atccgctatg gtgactgaat ctacagattt
    13501 ttttaatata aaagatgaca taatagaaaa aatgttagaa gttgttcacc ctacaaaggc
    13561 atgtgtgaat ctattttata aaaatgattc taaatatgcc tacttagtat gttcgcaaac
    13621 cttagaatat gtacctcaaa tttttccaat aaatagtctt aaaggtgcaa aaattgattt
    13681 tgacacatat ccagagtata tacatgaggc tgtaaatgaa aaaaagataa tatatgtgga
    13741 aaatgtattt gaagattcta gagcagaagg tgaacgagat ttagcaaaag ctgaaggtta
    13801 tattgggaga atagtttttc cttttataat aaataacgta gttgttggat ttatgacttg
    13861 ttttttaaaa gaggatgatt atataacaga acaagatata gattttatat catctgttgc
    13921 atcactctta agtttatcaa tagaaataac taataaaaat aataatgtac aaattttaat
    13981 tgataaacta agaggctcaa taagtgccat aaatgaagct actaaaaaac tataccttaa
    14041 taagagtatt gatggctttt tggaaaattt aagtaaacaa gcgtgtaata ttactaatag
    14101 taaagaagct ttaataatta taaatagtca tcataataag gatgctatag ttagttgtta
    14161 taatgttgag caaaaaaaga aaacaaattt atatcctatg attgatatgt ttttaaagaa
    14221 agagtcattg ggggggtatc taaataattt aaaaccatgc tcaaaaaagg gaataaattt
    14281 ggaaagttat atatattaca aacttcagga taaaaatgat actataggat gtatagtttg
    14341 tgcaaatagc aaaaactata ctagtgatga tttaaatatt cttagtatat tgtccaaaca
    14401 agtttctgtt ggtatgcaat tatatgaaca taatgaaaag gaagtaaaac acaaagttct
    14461 tgaaaacgag ttgaacatat taaataaaca acaacagctt attatggata aaggtaaaat
    14521 ggattgtaat gatgagaaag aactttattt ttaccataaa ccagcaaggg tagtaggtgg
    14581 agatttttat tatgctacaa aagtggatga cgaacatata gtttatattg ttgctgatgt
    14641 aatgggacat ggaatggtat caaattatgt tgttgcaatg ataaaaggtg cttttaaagt
    14701 attatgcaat caatacaata caccaagaga aataatgaca aatttaaata ggatgttata
    14761 tgatgaattt gacaagatgg gagtatttac aacttgtttg ataggtatta tagatactaa
    14821 agaaaatatg attactgtgt ctaatgcagg tcactatagt cctgtagttg taaagaaaga
    14881 tggcacaata gaaaataatt taaattgtaa aaagggcata cctattggaa taatggaaga
    14941 tgctacttat gaaaataata ctctttcaat ggaagattat gcaatggtat gtatgtacac
    15001 tgatggaatt ttagagataa aaaattcatc aaaagaagag tatggtataa accgattaga
    15061 aaactttcta aaagaaaatt ttagattaaa tcaacagtta atagtagaaa atctaaaatt
    15121 ggatttaaag aacttctcat caaaagataa ttatgatgat gatattttaa tagttatgtt
    15181 aaaggatagg tagattgatt atggattcaa acgataaaaa aggacttaaa agtccagaga
    15241 ttataataat aaatggaagt ccaagaatta ataaaaattg cagttttata gcaaatgaag
    15301 taaaagctat gctagaagag tgtggattaa gttgtaaaat atttaatatc tacaatatgg
    15361 atattgaata ttgcacagca tgtggatttt gtgaaaaaac agggtattgc aagtttaatg
    15421 atgatatgaa gccattatat aaaatgtttg atgattcaaa aggaactata gtaataagtc
    15481 ctgtacattt tgattcaata tcagctaaat taaagactgt tgttgataga actcaagcta
    15541 tatatgcaag taaatatgta ttaaataaac cttcaattga tagaagtaag aaacggatag
    15601 gaatgtatat tgcagtgggg gggagtaaat catatgattc acagtttaaa ggtggacaga
    15661 ttgtgatgga ttttttcttt aagagtataa atactaaact tttacacaat atatatttaa
    15721 acaatacaga tgatgtatct tatatagata atcaagaatt taaagatata atatataaaa
    15781 atttaaagat atatattgat tcaatataat ttgattttgt aggtaatatt atgtataatt
    15841 gtattgcaat aatttaaata attaaaagga gggaatttca atgaaattac caatagcatt
    15901 atctaataaa catgttcatt tgagtcaagc tgatatagac gtattatttg gggcaggtca
    15961 tgagttaact aaatttaaac cattatctca accagggcaa tatgcctgtg aagaaaaagt
    16021 ggatttagta ggaccaaaag gaactttaaa aggagttaga gtactaggac cagctagagc
    16081 tcatactcaa gttgaaatat ctttagcaga tggtttccca ttaggagtaa aagcaccaat
    16141 aaaagaatct ggtgatatag ctggaactcc aggtgttaaa ttagtaggac ctgctggaga
    16201 agttgaaatg aaagaaggag ttatagttgc ttctagacat atacatatga gcaatgaaga
    16261 tgctgctaaa tttggtgtaa ctgataagca aatagtaaaa gttaaaacat ctggaccaag
    16321 agcattagta tttgaaaatg tattagtaag agctagtgac agctttaatc tagaaatgca
    16381 tgttgatatg gaagagggaa atgcagcagg cgttaaaaat ggagatttag tagaattaat
    16441 agttgattaa tattataaat agactttatg ctagtagata ttttatttgc tagcatattt
    16501 ttaaatgtaa taaatttcta taggataaat ttaatcgatt agattaattt ataaaataaa
    16561 ggggtattat aaaaaatgta ataaaaactt acaaaaatat gatgtaatat ggtaaaatta
    16621 tatata
//
LOCUS       DAYATN010000079        16614 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.079, whole genome shotgun sequence.
ACCESSION   DAYATN010000079 DAYATN010000000
VERSION     DAYATN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.079"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(232..1761)
                     /locus_tag="ACGI8A_002399"
     CDS             complement(232..1761)
                     /locus_tag="ACGI8A_002399"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase domain-containing protein"
                     /protein_id="HFH0180394.1"
                     /translation="MKSLKALIVGVSKYSIVKNKDLLFCKNDIIEINNVLHKRLGITS
                     NNIRTLGMEYEVRKDLFFKTFQEISDSIGDDDTFIFYFSGHGCICNNGVHYLVLSDEL
                     VSIQFVIDFIEGLNCKNKIVFIDCCFAGKFNVSDKQKINIKDIFSLFDRKGCAVLVSS
                     SEYQESFSLPDSSISRFTSLLSEALRNEYIVRKGELSLRAISKYINLRMSILNKNYIN
                     CKQNPIFRSNILGDIYFNVKDYKSYIKKKVFQEHDDYIIYNVKSLHISIIKRYSVEVI
                     LKNKFSYCDISEISKEVFDIVKSVEVYNSEIGEDRLKGKDANIIWVYFGKDEIDMING
                     IYMCKTTWIDKYQEKDIWYSVNGKNKFFINGVHTETYDNYESLKSYISQNTITKEKAV
                     YEVKEILTNMINISEEIISFYNEYENKKICEDNIFQIIDSRKKKMDECYNKVINLDFP
                     PIEIKDWFDKCINLVSTIQNFGVYWDHSRTEENRKQCMEMNIEDYYKDLREVIRLSEE
                     I"
     gene            complement(2460..3890)
                     /gene="dcm"
                     /locus_tag="ACGI8A_002400"
     CDS             complement(2460..3890)
                     /gene="dcm"
                     /locus_tag="ACGI8A_002400"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFH0180395.1"
                     /translation="MKKITIDKNRVRKIMKEKNIRSQNQLALNMGITKNQLSAILSEK
                     FTPIKSNVEKLCEVLDIGFDEIMCLEDVNEKQLKFTNICSSNKDYIEHRDCTYKELSE
                     YELNPRERNKNNKHIDISNVLAKRAYTCVELFAGAGGLALGLEKAGFKEVGLVEWDKY
                     ACDTLKLNRPDWNVIQGDIVKIAENGIKNYIDNNIEIDLLSGGYPCQAFSYAGKKLGL
                     EDVRGTLFYSYAKILEELKPKVFLAENVRGLVSHDGGKTLKTMIDVFSEIGYNVKYEI
                     LKAVNYGVAQKRERVIIIGTRKDLSHVDFKFPKSFEYVATLRDALKNVPKSEGARYPE
                     RKKKVLDMVPPGGCWIDLPDEVARDYMGKSYYSGGGKRGMARRISWDEPCLTLTCSPA
                     QKQTERCHPDETRPFTTREYARIQSFPDEWEFTGSVSQVYKQIGNAVPVKLGKAIGLS
                     IVDYLNKIEKIYVTEEVAITEEEYVI"
     gene            4103..4819
                     /locus_tag="ACGI8A_002401"
     CDS             4103..4819
                     /locus_tag="ACGI8A_002401"
                     /inference="COORDINATES: protein motif:HMM:NF025864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HFH0180396.1"
                     /translation="MTELEKQEILDKAKDFFTNILVANHKKNTIKLNLKAFNINPFLV
                     KYLASFLTGSNKPEDIAKALIYPRVLGTSINTSFGTNLQKFCITTLDGFASTTSGIDI
                     EFIDCIDGRRKFCQIKAGPNTINKDDVKTIIDHFSSVKRLARTNGLSLSYNDLIVGVF
                     YGTENELNAHYKRINEEYPVVVGQDFWYRLTGDENFYFDLIDTIAQSALEEDCTVLLK
                     KTISNLADEISKDKSLFNQK"
     gene            complement(5064..5342)
                     /locus_tag="ACGI8A_002402"
     CDS             complement(5064..5342)
                     /locus_tag="ACGI8A_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6809 family protein"
                     /protein_id="HFH0180397.1"
                     /translation="MYDEIIEIYFDYWINEHMYRKVLEKDEYYNDRCKEIKKVNKLLE
                     KNLSEEKKMDVLIELNDLFNSVIEIERELGYKLGFKAGARFMIELLNS"
     gene            complement(5590..6924)
                     /locus_tag="ACGI8A_002403"
     CDS             complement(5590..6924)
                     /locus_tag="ACGI8A_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFH0180398.1"
                     /translation="MDNMSNLVKNTEAEQSILGSILLDSRKIEEIEILISEDMFYHQE
                     HKHIFNSVKDIHKRDEPIDIITLTEDLKKKGLIDYVGGISYLTKLSTIVPTIGNVSHY
                     IEIVIEKYKNRNIIDKFNKFKLGEIDDNTLIYQLEKYVNEVNQKLVKKDSSITAISER
                     AFEYINDEIEEGIDIGIKFLDETIGGLYSGELITIAAKSGRGKTALALQIMRNVVFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNEEEIIKYINALSTLNQENNLY
                     INDSVNTISGIKREIKSIKPDVVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPIIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALDYCKTMGIE
                     DIKELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGSLFSFKECKKVKEV"
     gene            complement(6937..7680)
                     /locus_tag="ACGI8A_002404"
     CDS             complement(6937..7680)
                     /locus_tag="ACGI8A_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180399.1"
                     /translation="MSNLGWIKLHRKIKESPLYKDCNSKQRDILINLLLSVNHKPNKW
                     VYGGKEYEVKSGQLITSLESIANMCSKDVSIQNVRTALKKFERYGFLTNKSTNKNRLI
                     TIENWELYQSNEENQQTNQHAINKQLTTNKNVNNEKSKIYSEIIEKFNITCTELSKVK
                     KLTEARKKVINARIKEYDKETVLEIIHIVSKTSFLNGSNEKGWKANFDWIMSPNNFIK
                     ILEGNYQDKSINVKVDAEAYSPDDSQLKW"
     gene            complement(7701..8015)
                     /locus_tag="ACGI8A_002405"
     CDS             complement(7701..8015)
                     /locus_tag="ACGI8A_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180400.1"
                     /translation="MADTNNLEVLKTLQATLNILVKQEQEAQEKEKGYKEIQTYRRDK
                     AQLITCKEASKIYPIGESKFRQLCHSKNKGFPCIWIDNRVYIIKNKLDNWFIENANGI
                     KL"
     gene            complement(8019..8213)
                     /locus_tag="ACGI8A_002406"
     CDS             complement(8019..8213)
                     /locus_tag="ACGI8A_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0180401.1"
                     /translation="MKLKLKRIEKGIKQYELAEKVGISRYYLSSLERGKSKNPSNMLM
                     LKLAQELDTSVEELFFDKEE"
     gene            8353..9213
                     /locus_tag="ACGI8A_002407"
     CDS             8353..9213
                     /locus_tag="ACGI8A_002407"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0180402.1"
                     /translation="MIDNTIGKKIKQYRKSINMSRDELANKIGCSVYAVAKYEQGQRK
                     PSLDMIKKISTALSIPINRLIIDESDLDTSTNNYTNLKELDLVVKTFDNYSAKLIGNN
                     ISKFRNINNISLKELSEKINCSENEIINYENGTKMPRIEILKKIALATYTKIEDLFGF
                     PLEEIKIDNQTILYRNPQTGYIIDNFLIDFINKDINIDNTPVSYYIDKSEFDLVIHIL
                     KNYGYDVKTDYKTKFEIADNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSN
                     GLIEDEDEEY"
     gene            9310..10062
                     /locus_tag="ACGI8A_002408"
     CDS             9310..10062
                     /locus_tag="ACGI8A_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFH0180403.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVSDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDEYNYKSYKELFKNGN"
     gene            10052..11218
                     /locus_tag="ACGI8A_002409"
     CDS             10052..11218
                     /locus_tag="ACGI8A_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0180404.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGAPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKIEAVEKLDKIFSL"
     gene            complement(11335..11565)
                     /locus_tag="ACGI8A_002410"
     CDS             complement(11335..11565)
                     /locus_tag="ACGI8A_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180405.1"
                     /translation="MEANKTLLQSLYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGISDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(11814..16385)
                     /locus_tag="ACGI8A_002411"
     CDS             complement(11814..16385)
                     /locus_tag="ACGI8A_002411"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFH0180406.1"
                     /translation="MTFEEIMEFNKPYNQKNFDELVNAIKDGYVVPYIGAGMSMLFKD
                     IYPSWSTFLNNTSEEYLEDEDKILFEKIEDYEKKAEFLYEELAKVRFSKHLKKVFGAG
                     YLDAKDKDDFIDKCVYLIPIIFGSGLIITTNYDKVLEKNYGLHNKTVLVSHPGHFEAL
                     NNALRNNELVLYKIHGDITEPTTSIVLTKEQYEKAYSNDELRKMLTQVYTSKELLFLG
                     CSLEKDRPLELLQQVSEAGTGHYAIIPVSSEKKKHRRIELENEYYIQTIMYPEGKHEC
                     VRIILERILEIVLPDEYRRLKDRSLKESRLTNLSCRLTEKWFQNQNNIQIKNLGDRYL
                     PELNVELSLENTFNALGKNEEFKQRFDEKTDKVLSSIKKQKINEVEVDVLVIEEIIQQ
                     FYDKANERLDIKLLKKHFEKISAALDDRINNFKNIIINEDCEKSKKREDEIYKLKQTQ
                     KDIYVYIKYLDSKEIKVVNSPYILLHSAGGMGKSHIIAHTIEKRNMDGHKSILFLGQH
                     FKLDKTPIKHMLEILEIECEFEELLEILNEIGEKEQQRVILFIDALNEGQGKELWKYY
                     LNGIVEQIKKYPWIGLILSIRSEYIPSLIDNNNKIKENLVFIEHQGFSSIEHKAMKKY
                     FDYYGIPYSNIPMPNQEFRNPLFLRIMCETFKGEEINLDTISFTDIYKNYLNILNLRI
                     SESCNYSRHANVVTEVLNEMIKYKYGDGLGNNLIPANKSVEIIINIASKYKINNVIID
                     ELIANGIVNKTVNLNDEEYMHVTYEKLEDYLYANLLVKELKEIGVVDFSHKYKYLVEY
                     EDILEVLAIVLSEETGSDIPNEIFEVFKNKETNYNVISAFINALKWRKKSTITEKTLD
                     YINKIVLGKSYYKIDFYNVLILISTKVNHTLNADYIVKYILQNTMADRDAIFIPIFNE
                     VYEEEESSIKRLLGWCFDFEGFDNTSNETIRLSAMMIATFLISSNRDLRDKSTKALVN
                     LLTGKMDIVIDLLKTFEDVDDPYIIERLYAVAFGCVVSEQSEEMIEALAVYVYNKIFD
                     VDYVYPNILLRDYAKNIVDYAKYKIDSSVLKDMNVEPPYKSIFPKVPTDEEIEGYKYD
                     YKASDFKDYYWSQNTILNSMEVEYTRDGQPGWYGDFGRYMFQHYFHKWKGLDYNDLKN
                     IAIKKVFDMGYDVNKHGKFDRTVQNGRLRDSKYERIGKKYQWIALYELAAQAADNYKI
                     EVYTDAYGTKKEIYCKGTFEPDIRNIDPTVMTVINNNNNKRVHNQLFQFSPIPNNEWL
                     GDFSDLPSINDMVNSVYKNQDFLLLNGWYTWKEENDFKNKNYKNPEKDMWVLINSYII
                     KNDQLEPIVNALKGKDFMGRWLTEPNSNYTLYNKEYYWADGYYFFQNSYYCGEEWVQI
                     DEHKNILEKDYKVLLPTYSYTTEREGDIFQEDFSGTWHKPCSTLFYGLKLRYGQGDSI
                     LYNDYGEVVCFESSELLNEEIGFFINKEIFLKYLNEQGYSVFWTMLAEKRVLEEPYMD
                     RKYIQPTISGVYTLNGENRLVGETIESEY"
BASE COUNT         5638 a         2699 c         1656 g         6621 t
ORIGIN      
        1 ttataaaagt tccattaaca gcatattata cgtttttaaa tatttactag actttttcaa
       61 aataaatatc taattattta aatcaatacc aatataattt ataaatggat ttataattgt
      121 atcttctatc atgtttagtg taaacaactt attttaattt aaattcgtaa ccaacaaagt
      181 accaatctga cttataattc tatattagta caactatgga aaaatttaat tctatatctc
      241 ctcacttaat cttatgactt ctcttaaatc tttgtaataa tcctctatat tcatttccat
      301 acattgcttt ctattttcct cagttctact atgatcccaa taaactccaa aattttgtat
      361 agttgacact aagtttatac atttatcaaa ccaatcttta atttcaattg gtggaaaatc
      421 taaatttata actttattgt aacactcatc catttttttc tttctactat ctataatttg
      481 aaaaatatta tcttcacata ttttcttatt ttcatattca ttatagaagg atataatttc
      541 ttcagatatg tttatcatat ttgttaaaat ttctttaact tcgtatactg ccttttcttt
      601 agtgattgta ttttgactta tatatgattt taagctttcg tagttatcat atgtttcagt
      661 gtgaacaccg ttgataaaaa atttattttt cccatttaca ctataccata tatctttctc
      721 ttgatattta tcaatccaag tagttttaca catatatatt ccatttatca tatcaatttc
      781 atctttgcca aaatataccc atattatatt agcatctttt ccttttaatc tatcttctcc
      841 tatttcacta ttataaactt caacagactt aactatgtca aaaacctcct tagatatttc
      901 tgatatatca caataggaga atttattttt taatataact tctactgaat atctttttat
      961 tatacttata tgtaaagatt tgacattgta tattatgtaa tcgtcatgct cttgaaaaac
     1021 tttctttttt atataagatt tataatcttt tacattaaaa tatatatctc ccagtatgtt
     1081 agacctaaag ataggatttt gtttacagtt tatataattt ttgttcaata tactcatacg
     1141 caaattaata tacttgctta ttgctcttaa agataactcc ccttttctta ctatgtattc
     1201 atttcttaac gcttctgata ataaacttgt aaatctacta attgaactat ctggaagaga
     1261 aaatgattct tgatattcgc tacttgatac taacactgca catccttttc tatcaaacaa
     1321 ggaaaatatg tctttaatat ttattttttg tttgtcactt acattaaatt tacctgcaaa
     1381 gcaacaatca ataaatacaa ttttattttt acaatttaat ccttctataa aatcaattac
     1441 aaattgaata gaaactaatt catcacttaa tactaagtaa tgcactccgt tattacatat
     1501 gcatccatgc cctgaaaagt aaaatataaa tgtatcatca tctcctatag aatcgcttat
     1561 ttcttgaaaa gttttaaaaa ataaatcttt tctgacttca tactccatac ctaaagttct
     1621 tatattattc gaagtaatcc ccagtctttt atgtaacaca ttattaattt caattatatc
     1681 atttttacaa aatagtaaat ctttattttt aactatacta tatttactta ctcctacaat
     1741 taatgctttt aagcttttca tattccattc cccctttata atttttcaat ttaatatact
     1801 taaaatgatg ttttaattgt tttattgatt ttataaaagc tagtatcaca ctactttttt
     1861 gactttttca ttatttactt ttgtagttgg attataattt ttcaaggtaa gtatatatat
     1921 atttatttac atcgcaccca tataatttat atctctgatt tatctgacct gaacttagtg
     1981 taatttaaac caatgtaaaa tcatctatct cttcaccata tattttttaa aattccccaa
     2041 aatattgatg ttaatagaat ttcatgtgtt ttttgtattc tactatccat aagaatttta
     2101 aagtgagtaa atatttccaa tcatttatat cttatttaat atcattttat tccctcaagt
     2161 atttatttaa attgttatgc tttatttata aaattataca tacatttcta aagttagaca
     2221 tacatcttta aaaaccttta atttgtgcta ctttttttct aaaaataata aatatataaa
     2281 aattatatat acaaaaaatt aagttatgac tagtttataa ataagtacat aataaaatta
     2341 aagagagcaa ttttttttgc tctctttcaa aatttatatt aaattgtaat aataaaaata
     2401 aaaacgctag ttatctaagg ctaatatttt caacgattat ataaatatct acataacagt
     2461 taaattacat actcttcttc agttattgca acttcctcag tgacatatat tttttctatt
     2521 ttattaagat aatctactat acttaatcca atagcctttc ctagcttaac aggaactgca
     2581 tttcctatct gcttgtacac ctgactaact gaccctgtaa actcccactc atcaggaaaa
     2641 ctttgaattc tagcatactc tctagttgtg aatggtctag tctcatctgg atgacacctt
     2701 tcagtttgct tttgtgcagg actacatgtt aaagttaggc atggttcatc ccaacttatt
     2761 cgccttgcca tacctctttt tccgccaccg ctataataac tttttcccat ataatctctt
     2821 gccacctcat ctggcaaatc tatccaacac ccacctggtg gtaccatatc taacactttt
     2881 ttcttcctct caggatatct agctccttcc gattttggaa catttttaag tgcatctctt
     2941 aacgtagcaa catattcaaa cgacttaggg aacttaaaat ccacatgtga caaatctttt
     3001 cttgttccta ttataataac tctttccctc ttttgtgcaa ctccatagtt cacagccttt
     3061 agtatctcat attttacatt gtatccaatc tcgctaaata catctatcat agtctttaat
     3121 gtttttccac catcatgaga tacaagacct ctaacattct ctgctaaaaa caccttaggc
     3181 tttaactctt ctagaatttt agcatatgaa taaaacaatg tccctcttac atcctctaac
     3241 cctagcttct tacccgcata gctaaatgct tggcatggat aaccacctga tagcaaatct
     3301 atctctatat tattatcaat ataattcttt attccatttt ccgctatttt aacaatgtca
     3361 ccctgtataa cattccagtc tggtctattt aattttaatg tatcacaagc atatttatcc
     3421 cattctacta atccaacctc tttaaaacca gctttttcaa ggcctaatgc taatcctcct
     3481 gctcctgcaa ataattcaac acatgtatat gctcttttag ctagtacatt acttatatct
     3541 atatgtttat tatttttatt tctctctcta ggatttaatt catattcact gagttcttta
     3601 tatgtacagt ctctatgctc tatataatct ttattactgc tacaaatatt tgtaaatttc
     3661 agttgctttt catttacatc ttccaaacac ataatttcat cgaatccaat atctaacact
     3721 tcacataatt tttctacatt actttttata ggtgtaaatt tttctgataa tattgcagat
     3781 aactgattct tagttatgcc catatttaat gccagttgat tctgacttct tatatttttc
     3841 tctttcataa tttttctcac tctattttta tcaatagtta tttttttcat ttagttcacc
     3901 tcttaataaa taatataccc ttaaatagtt aataaaatac attaactatt taaggatata
     3961 ttatcataat tctaattttt atgtaagtag ttataaaaac ttaaaataaa atatctgtaa
     4021 acaacctaat attacctcat aattttacac attatggaat atattgtata attacattaa
     4081 tactttttag ggggattatt ttatgactga attagaaaaa caagaaatac tagataaagc
     4141 caaagatttt tttacaaata ttttggttgc taatcataaa aagaatacta ttaagttaaa
     4201 tttaaaggca tttaatataa atcctttttt agttaaatac ttagctagtt tcttaacagg
     4261 atcaaataaa cctgaagata ttgctaaagc attgatttat ccaagagttc taggcacttc
     4321 gattaatacg tccttcggaa ctaatttaca aaaattctgt attaccactt tagatggttt
     4381 tgcctctaca acttctggca ttgatataga gtttattgat tgtattgatg gtagaaggaa
     4441 attctgtcaa attaaagctg gaccaaacac tattaataaa gacgatgtta aaactataat
     4501 tgaccacttt agctcagtta aaagacttgc tagaacaaat ggactttcat tatcttataa
     4561 tgacttaatt gttggtgttt tttatggaac agaaaatgag cttaatgctc actataaaag
     4621 aattaatgaa gagtatccag tagttgttgg tcaagatttt tggtataggc ttactggtga
     4681 tgaaaatttt tattttgatt taatagatac aattgctcaa tctgctttag aagaggactg
     4741 tactgtttta cttaaaaaaa caatatccaa tctagctgat gaaatttcta aagataaatc
     4801 tttattcaat caaaagtaat ataaaaatga ttaaatagca taatttacat ttttagatat
     4861 ttaaaaataa tattcaccaa acaaaattat taagtaaaat gaaatgtatc ttataaaagc
     4921 tgagctataa gtaaatcttt gtaaaaacaa atttacttat agctttttta tgttcaaaaa
     4981 acacaaatta tatactattt aatatattat aaacttttat ttgttaatac tctctactaa
     5041 atttctaaca tctcttatat aaattaacta tttaataatt caatcataaa tctagctcct
     5101 gccttaaaac caagcttata accaagctct ctttcaatct ctataactga attaaataaa
     5161 tcatttaact ctatcaatac atccattttc ttttcttctg ataaattttt ttctagtaat
     5221 ttattaactt tcttaatttc tttacatcta tcattataat actcatcttt ttctagtact
     5281 ttcctataca tgtgttcatt tatccaataa tcaaaatata tttctataat ttcatcatac
     5341 atgttagtac ctccttgact ttcatctggt acttaatata tacttaactt gtgtagatta
     5401 gtgtacatta agtacctaat ttataaaggt agttgttata tttatatggc aactaccttt
     5461 ttctaataaa tttttcaatc taaatactat atattttaaa tcttcattgc ttactattga
     5521 ggattatata cttttcttct aacactcctt aaacaatagt ttatatatca cttaataaat
     5581 tatatttttt catacctcct ttaccttctt acattcctta aaagaaaaca aacttcctat
     5641 ataccaataa ggtctaaagc ctgattcccc atctctattt ttagcaacta taatttcact
     5701 taaattagct ccaaaacctt tttctctacg tcttaattct tttatatcct ctattcccat
     5761 tgtcttacaa taatcaagag cctctttctc tgtaggtcta tgaatatata tgactgaatt
     5821 agaatccata tatatctgtt ttgaacttct cattactcta tctccttttg gcctttcatc
     5881 cttcaattca tcatttaatt gagccaactg tataattgga atattaaagt ctaaagtaat
     5941 cttttttatt tgccttgata aatcagcaaa cttagcttct ccaacttctt taccttcgta
     6001 atccaaaatc tgaagataat caattataac aacatctggt ttaatacttt ttatttctct
     6061 cttaattcct gatattgtat tgactgaatc attaatatag agattatttt cctgatttaa
     6121 agtacttaaa gcatttatgt atttaattat ttcttcttca tttagatttc tattcttcat
     6181 ttttacaact gatattccag ttaaagaagc tatgtttcta aacaaaattt gtatatcact
     6241 catttcccca cttataaaaa gtactttttt accttgaaat accacatttc tcattatctg
     6301 taaggctagt gctgtttttc ctcttccact tttggctgct attgtaatta attcaccact
     6361 atatagacct ccaatagttt catctaaaaa ttttattcct atatcgatac cttcttcaat
     6421 ttcatcattt atgtattcaa aggctctttc tgatatagct gttatactag aatccttctt
     6481 aaccaatttc tgattaactt cattaacgta tttttctaat tgatatataa gtgtattatc
     6541 atctatttca cctaatttga atttattaaa tttatcaatt atatttctat ttttatattt
     6601 ttcaataact atctcaatat aatgggatac attacctata gttggaacta ttgttgatag
     6661 ttttgtcaaa taacttatac ctccaacata atctataagc ccctttttct ttaaatcttc
     6721 tgtaagagtt attatatcta taggctcatc ccttttatgt atatctttca ctgaattgaa
     6781 tatatgtttg tgctcttgat gataaaacat atcttcactt attaatatct caatctcttc
     6841 tattttccta gaatctagta aaatactacc taatatagat tgttctgctt ctgtgttctt
     6901 aactaaatta ctcatattat ccataatata aactccctac cactttaatt gtgaatcatc
     6961 tggactatat gcttctgcat ctactttgac attaattgat ttatcttgat aattgccttc
     7021 taatatctta ataaaattat ttgggctcat tatccaatca aaatttgcct tccaaccttt
     7081 ttcattgctt ccatttaaaa agctagtttt actaactata tgaataattt ctaatacagt
     7141 ctctttatca tattctttta ttctagcatt aattactttt tttctagctt ctgtaagttt
     7201 ctttactttt gatagttctg tacaagtaat attgaatttt tctataattt cactgtatat
     7261 tttacttttt tcattattta cattcttgtt agttgttagt tgcttgttaa ttgcatgttg
     7321 attagtctgt tgattttctt cattagattg atatagctcc cagttttcaa tagttataag
     7381 cctatttttg tttgttgatt tatttgttag aaatccatat ctttcaaatt tctttaaagc
     7441 agttcttaca ttctgaatac ttacatcttt tgaacacata tttgctatac tttccaaaga
     7501 tgttattaac tgtcctgatt taacttcata ttctttacct ccataaaccc atttattagg
     7561 cttatgatta actgacagta ataggtttat gagtatatct ctttgtttgg aattacaatc
     7621 tttatatagt gggctttctt ttatctttct atgaagcttt atccaaccta gattactcaa
     7681 atactatcac ccacttaagt ctataatttt ataccatttg cattttcaat aaaccaatta
     7741 tctagtttat tttttattat atacactcta ttatctatcc atatacaagg aaatccttta
     7801 tttttagaat gacataactg tctaaatttt gattccccta tgggataaat tttagaagct
     7861 tctttacaag tgataagttg agctttatct cttctatatg tctgtatctc cttgtaccct
     7921 ttttcctttt cttgtgcttc ttgttcttgc ttgacaagta tattcaatgt agcttgtagt
     7981 gttttaagaa cttctagatt atttgtatct gccatacatc attcctcctt atcaaaaaat
     8041 aattcttcta cacttgtatc tagttcttga gccaatttta acatcaacat attacttgga
     8101 ttttttgatt tacctctttc taaagaagat aaataatatc ttgagatgcc aactttttca
     8161 gctaattcat attgctttat acctttttca attcttttta atttgagttt cattgatttc
     8221 ctcctttctt tcatacccat tttgtgtatc ctcatttcta taaaatattc taacacccat
     8281 ttggtgtatt gtcaatatat ttaagtgtat caatagaccc atttagtgtt ataatgtatt
     8341 tgaggtgaaa ttatgataga taatacaatt ggaaaaaaga ttaaacaata tagaaaatct
     8401 ataaatatgt caagggatga gttagctaat aagattggtt gctcagttta tgctgtagct
     8461 aaatatgagc aaggccaaag aaagcctagt ttagatatga taaaaaaaat atctactgca
     8521 ttatcaattc ctataaatag gcttattatt gatgaaagtg atttagatac ttcaacaaat
     8581 aattatacta atttaaaaga attggattta gttgtaaaaa catttgataa ttactcagca
     8641 aaactcatcg gaaataatat aagcaaattt agaaatataa ataatatatc tctcaaagaa
     8701 ctctcagaaa aaataaattg ttctgagaat gaaattataa attatgagaa tggaacaaaa
     8761 atgcctagaa ttgaaatatt aaaaaaaata gcacttgcta cttatactaa gattgaagac
     8821 ttatttggat ttccattaga agagataaaa atagataacc aaacaatcct ctatagaaat
     8881 ccccagactg gatatattat agataacttt cttatcgatt ttataaataa agatattaac
     8941 atagataata ctccagtttc ttattatata gataagtcag aatttgattt agtaattcat
     9001 atattaaaaa attatggtta tgatgtaaaa acagattata agacaaagtt tgaaattgca
     9061 gataatgcag atacaataaa accaattagc ttaagcttct ctgacttcaa agacttctcc
     9121 aaaaatctaa attgggtgat aaatggtctt gttgaaaatt ttatacaaga aaactctaat
     9181 ggtcttattg aagatgagga tgaagaatat tagtattgca cattttcaaa atatgagttt
     9241 acctattaac ttttatacat tcatagttaa taaataaaaa ttagatatga tatgaaagga
     9301 gctaaaatca tgaataaatc atatatacca aaatacacac ataaattccc tactaatcta
     9361 cctcatggag ataaaaaata tactttaaca tacattagaa gaatgataag tgaatttgta
     9421 tatgatatgg gtaacctaga aaataacccc tttacattcc cagaagtaca aacattactt
     9481 gatggtataa ccgtaggtgg acataaatta agtgaccaaa atcaaatact aaatataaaa
     9541 tcatcatggg attatgtaat aaaattaagt aacaaagaaa acttatcttt aaataaagat
     9601 acaatttgct ctatacataa aatagttgca aaagatgaag ctctaatagt tggagatttt
     9661 agaaatggta acataggtat agctggtact actcaatatg aatgcataga agccactcta
     9721 ttaaatgatt tatttgtaag cgacataaat atcataaata aaataactaa ccccttagaa
     9781 aaggctataa taataaattt atggttgtcc tattgccaat tcttctatga tggcaacaaa
     9841 agaactgcta gacttacctc aaacctaata cttataagta atgatatagg agttctatca
     9901 ataccagcta aacataaaca agaatacaac acattaatgc taaactttta tgaaacacta
     9961 gaagctgatg aagttataaa attcttatta gagaaatgta ttacattctt tgatgaatat
    10021 aactataaat catataagga gctgtttaaa aatggcaact aaaagagcta atggagaagg
    10081 ctctatcgga aaatataaag atggttggcg ttcccgtata atgattggct ataatgaaga
    10141 tggtaaacca ataagaaaag aattttatgg taagactcaa aaggaagtta aagataaact
    10201 tgatgcttat aaaaaacaat attatttaag ctctgatatt tcagatgata aaataacttt
    10261 agagcattgg ttttacaatt ggttatttga atatagaata aaagatttaa aacctaaatc
    10321 atttgaaaga tatgaaagca tctatcgtaa ttatattaaa gatactgatt taggcaatgt
    10381 taaattaaag gacttaagag tatctcacat acaaaaatat tacaatagat tgcttgattc
    10441 caataaaagt atacctacta taaggcaaat aaacactaaa ctgaaaacct gcttatcaga
    10501 agctgaaaaa caaggtctta tacaacgtaa ttattgtact atggtaaata taccagttga
    10561 gaaaaaggag aataagcttg aaatattgac tcttgaacaa caaaagagtt ttataaaagc
    10621 tatagatgga cataaattag aggtattatt tttacttgct cttggtactg gacttagatt
    10681 aggagaatta ttaggactta aatggtttga tattgatttt aaaaagtcta atttaacagt
    10741 taaaagaacc cttcaaagaa catattttat agataaaacc ggaaatagag agcttaaagt
    10801 attagagcaa gagccaaaga catcaaattc ttacagaact gtacctattc caaaagatgt
    10861 tttaaataaa ttgaaagagc ataaagtaaa tcaaaattcg gatatattaa aggctggaga
    10921 attatataga aatgataatt atgtattttg caatgaatta ggtgccccta ttgatgataa
    10981 aagaccttta aggaatctaa aatctatatt aaacagctta gatatagagc ctataaaatt
    11041 tcatggtctt aggaaaactt atgcgactag actttttgag aatgatgtcc ctcctaaaac
    11101 tgtacaagtt ttaatgggtc attatgatat atcaataact ttaaatatat atactcaagt
    11161 tatggaagat aagaaaattg aagctgtaga aaagttggat aaaatatttt cattgtaaaa
    11221 atcaaatata taaaaatact ctgatttcat atagttaatt acaaaaccaa aataaggata
    11281 caaattaatt ttatattatc ttgtatcctt attcttgtct agttttttaa atatttatct
    11341 tggatagcct ttatgataca ttaatccatt ttctaacatt agttcttggg ctaaatatat
    11401 atgaccctta cagtgcatat ctgatatacc ctcacttata agtttatatg tttcagaagt
    11461 atataaataa tccatacttt cttccaagct cttacctgtt tgtttagaaa attctaatat
    11521 gattttttta tatagcgatt gtaacaatgt cttattagct tccataaaag ctatacctcc
    11581 tctttcaaat atttgtttat cctattttga ctaactatat atttattagt tatttaatta
    11641 gccataataa ttcggctata agatatttat aatctaatct caattatata aatctttttg
    11701 ccaatattta ataatcaaaa tattgcactt aattaactat cttataagta ttgataataa
    11761 aatctgtctt cttaaatata cttaaataaa acttctataa ttatatcaaa gtattaatat
    11821 tcactctcta ttgtctctcc tacaagtcta ttttctccat ttagggtata tactcctgat
    11881 atagttggtt gtatgtattt tctatccata tatggttctt ctaaaactct tttttctgct
    11941 agcatcgtcc aaaatacact atatccttgt tcattaagat attttaagaa aatttcctta
    12001 ttaataaaga atcctatctc ttcattaagt aattcactac tttcaaaaca aacaacttca
    12061 ccataatcat tatatagaat cgaatctccc tgaccatatc tcaatttcaa cccataaaat
    12121 agagtactac aaggtttatg ccatgtgcca gaaaagtctt cttgaaatat gtcaccttct
    12181 cgttcagtag tataactata agttggcaat aatactttat aatccttttc taagatattc
    12241 ttatgttcat ctatttgtac ccactcttct ccacaataat atgaattttg aaaaaagtaa
    12301 tatccatctg cccaataata ttccttatta taaagagtat aattactatt aggttcagta
    12361 agccatctac ccataaagtc ttttcccttt agagcattca ctattggttc tagttggtca
    12421 tttttaatta tatagctatt gattaatacc cacatgtctt tttcaggatt tttatagttc
    12481 ttatttttaa aatcattttc ctccttccaa gtataccatc cattaagtaa taaaaagtct
    12541 tgatttttgt aaacactatt taccatatca tttatgcttg gtaaatcact aaaatctcct
    12601 aaccactcat tattaggtat tggagaaaat tgaaataatt gattatgtac tcttttattg
    12661 ttattattat ttattactgt catgacagtt ggatcaatat tcctaatatc tggctcaaat
    12721 gtacccttac aataaatttc ttttttagta ccataagcat ctgtatacac ttctatctta
    12781 taattatcag cagcttgcgc tgcaagttca tataatgcaa tccattgata tttctttcct
    12841 attctttcat atttactatc tcttaatcta ccattttgta ctgttctatc aaatttacca
    12901 tgtttattta catcatatcc catatcaaaa actttcttaa tagctatatt ctttaaatcg
    12961 ttatagtcta gacctttcca tttatggaaa tagtgttgga acatatatct tccaaaatct
    13021 ccataccatc ctggttgtcc atctctagta tattctactt ccatagaatt taagatagtg
    13081 ttttgactcc aataataatc tttaaaatct gatgccttat agtcatactt atatccctca
    13141 atttcttcat cagttggtac tttaggaaaa attgatttat atggtggttc aacattcata
    13201 tcttttaata ctgaactatc aattttatat tttgcataat ctacaatatt ttttgcataa
    13261 tctcttaata aaatattggg ataaacataa tctacatcaa atattttatt ataaacataa
    13321 actgccaatg cctctatcat ctcttctgat tgctctgata caacacatcc aaatgcaact
    13381 gcatataatc gttcaataat atatgggtca tctacatctt caaatgtttt aagtaaatct
    13441 attacaatat ccattttccc tgttaataga tttacaagag cttttgtact tttatctctt
    13501 aagtctctat ttgaagaaat taaaaacgtt gcaatcatca tagctgacaa tctaattgtt
    13561 tcatttgaag tattatcaaa accttcaaaa tcaaagcacc atcctaataa tcgcttaata
    13621 gaagactctt cttcttcata aacttcatta aatataggta taaagatagc atctctatct
    13681 gccatagtat tttgtaaaat atattttact atataatctg catttaatgt atgattaacc
    13741 tttgtagaaa ttaagataag tacattataa aaatctattt tataataaga tttacctaaa
    13801 actattttat taatataatc tagtgttttt tcagtaattg tagatttctt acgccatttt
    13861 agtgcattga taaatgcact aataacatta taatttgttt ctttattttt aaaaacttca
    13921 aaaatttcat taggtatatc tgagcctgtc tcttcagata aaacaattgc caatacttct
    13981 aatatatcct catattctac taaatattta tacttatgtg aaaaatctac taccccaatt
    14041 tcttttaact ctttaaccaa caaatttgca tataaataat cttctaactt ctcatatgtc
    14101 acatgcatat attcttcatc atttaaattg actgttttat taactattcc atttgctatg
    14161 agttcatcta ttatcacatt gttaatctta tacttacttg caatatttat tataatctct
    14221 acactcttgt ttgcgggtat taaattattc ccaagaccat ctccatattt atatttaatc
    14281 atttcattaa gtacttctgt aactacattg gcatgtctac tataattgca tgattcagat
    14341 attctaagat taagtatatt caaataattt ttataaatat ctgtaaaact aatagtatct
    14401 aagttaatct cttctccttt aaaagtttca cacataattc tcaaaaaaag agggtttcta
    14461 aattcttgat ttggcattgg aatattagaa taaggtattc cataataatc aaaatacttt
    14521 ttcatagctt tatgttctat actagaaaat ccttgatgct ctataaaaac caaattctct
    14581 tttatcttgt tattattatc tattaaagat ggtatatatt cacttcttat tgataatatt
    14641 agtccaatcc atggatattt ctttatctgt tctacaatac catttaaata atatttccaa
    14701 agttcttttc cttgaccttc attcaatgca tctataaata atatcacacg ctgttgctct
    14761 ttttctccta tttcattaag tatttctaat aattcttcaa actcacactc aatttctaat
    14821 atttctaaca tatgtttaat tggtgtttta tccaacttaa aatgttgccc taaaaataat
    14881 atagacttat gcccatccat atttcttttc tcaatagtat gtgcaattat atgagatttc
    14941 cccataccac ctgcactatg taataaaata taagggctat taactacttt tatttcctta
    15001 gaatccaaat attttatata tacatagata tctttttgag tctgcttcaa tttgtatatt
    15061 tcatcttctc tttttttact tttttcacag tcttcattta taattatatt tttaaaatta
    15121 tttattctat catcaagtgc tgcacttatt ttttcaaagt gcttttttaa caacttgata
    15181 tctaatcttt cattagcttt gtcataaaat tgttgtatta tttcttctat aactagtaca
    15241 tctacttcca cttcatttat tttctgtttc tttatacttg atagaacttt atctgttttt
    15301 tcatcaaaac gttgtttaaa ttcttcattt tttcctaatg cattaaacgt attttctaaa
    15361 ctcaattcta cattcaattc tggtaaatat ctatctccaa ggttttttat ctgtatatta
    15421 ttctgatttt gaaaccattt ctctgttaat ctacaagata aattagttaa cctactttcc
    15481 tttaaagacc tatcctttaa tcttctatat tcatctggca aaactatttc caaaattcgt
    15541 tcaagtatta ttcttacaca ctcatgctta ccttcaggat acataatggt ttgtatataa
    15601 tactcatttt ctaactctat tcgtctatgt ttctttttct cactactaac tggaataata
    15661 gcataatgac ccgttcctgc ctctgatact tgctgtaata attccaacgg tctatccttt
    15721 tccaaactac atccaagaaa taacaactct ttacttgtat atacttgtgt aagcatcttt
    15781 ctcaattcat cattactata tgctttttca tattgttctt ttgttaacac aatagatgtt
    15841 gtaggctctg taatatcccc atgaatttta tacaatacta attcattatt tctaagtgca
    15901 ttatttaatg cttcaaagtg acctggatgt gacactaaaa ctgttttatt gtgtaatcca
    15961 taattttttt ctagtacttt atcataatta gtagttataa ttaatcctga gccaaaaatt
    16021 ataggaatta aatagacaca tttatcaata aaatcgtctt tatcttttgc atccagataa
    16081 cctgccccaa aaactttttt taaatgttta gaaaatctaa ctttcgccaa ttcttcatat
    16141 aaaaattctg cttttttctc ataatcctct attttttcaa ataaaatttt atcctcatct
    16201 tctaaatatt cttcacttgt gttatttaaa aatgtactcc atgaaggata tatgtcttta
    16261 aataacatag acatgcctgc tccaatatat gggactacat aaccatcttt aattgcattt
    16321 accagttcat caaaattttt ttgattgtaa ggcttattaa attccataat ttcttcaaat
    16381 gtcattattc cctcctaaac tatctcttaa taatttcaac cataattcct attttataat
    16441 tttattatat cattccattc cattttttta ctacaattct atgttataga atgaattcac
    16501 ttacttacat ttgttaataa tataaaactt tcttttaaga taattaaaat ttatactttt
    16561 aaattatgga taaaaatgga atgttataac ttttaatgta ttactaatat taac
//
LOCUS       DAYATN010000080        16607 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.080, whole genome shotgun sequence.
ACCESSION   DAYATN010000080 DAYATN010000000
VERSION     DAYATN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.080"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            278..2095
                     /locus_tag="ACGI8A_002412"
     CDS             278..2095
                     /locus_tag="ACGI8A_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFH0180407.1"
                     /translation="MLIFISFISIKLIHNVGDYGKLINYVGIVRGASQRLTKLEMNHQ
                     PNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKWEDLNIEIKKVRMKE
                     QNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISIIGILACIILILQYSK
                     KMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDKKFAVFYIDFENFKY
                     INDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRCYVDKEDLVVRQRDV
                     DNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANFAQKTVKNKPGTNYA
                     FYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKINCAEALVRWLTPDK
                     GIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFVQISVNVSRIQFYNT
                     KFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLHENGFTCSLDDFGKG
                     YSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKELNITTVAEGIECKE
                     QVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2387..2986)
                     /locus_tag="ACGI8A_002413"
     CDS             complement(2387..2986)
                     /locus_tag="ACGI8A_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFH0180408.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3089..3886)
                     /locus_tag="ACGI8A_002414"
     CDS             complement(3089..3886)
                     /locus_tag="ACGI8A_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0180409.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3999..4104)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4196..5182)
                     /locus_tag="ACGI8A_002415"
     CDS             complement(4196..5182)
                     /locus_tag="ACGI8A_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFH0180410.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     regulatory      5346..5522
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5666..7396
                     /locus_tag="ACGI8A_002416"
     CDS             5666..7396
                     /locus_tag="ACGI8A_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFH0180411.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLSIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7506..7586
                     /locus_tag="ACGI8A_002417"
     tRNA            7506..7586
                     /locus_tag="ACGI8A_002417"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7540..7542,aa:Leu,seq:tag)
     gene            7921..8436
                     /locus_tag="ACGI8A_002418"
     CDS             7921..8436
                     /locus_tag="ACGI8A_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HFH0180412.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8928..9032
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9242..11716
                     /gene="gcvPA"
                     /locus_tag="ACGI8A_002419"
     CDS             9242..11716
                     /gene="gcvPA"
                     /locus_tag="ACGI8A_002419"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HFH0180413.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11716..13173
                     /gene="gcvPB"
                     /locus_tag="ACGI8A_002420"
     CDS             11716..13173
                     /gene="gcvPB"
                     /locus_tag="ACGI8A_002420"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HFH0180414.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13596..15953
                     /locus_tag="ACGI8A_002421"
     CDS             13596..15953
                     /locus_tag="ACGI8A_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFH0180415.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(16039..16356)
                     /locus_tag="ACGI8A_002422"
     CDS             complement(16039..16356)
                     /locus_tag="ACGI8A_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFH0180416.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         6282 a         1924 c         2707 g         5694 t
ORIGIN      
        1 ggtttgtaat taaaaagttt ataaattata ttaatagtaa taatctataa gaacttatat
       61 attagaaaaa aatttttgta tatataaagg actctaaaga tgaactatgt aaaacaaggt
      121 ttatatattc taattgtata gaaaaattta ttgtaaacgc atgtatatta ttagaaaaaa
      181 tagtttggag gtattcaata aacataaaaa acaatatagg ggataaaggt gttttatgaa
      241 gagatttttg aggagaatta tcttagtttt atttattttg ttgattttta taagttttat
      301 ttctattaaa ttaattcata atgttggaga ttatggaaaa ttgattaatt atgttgggat
      361 tgttcgtgga gctagccaac gtctaactaa actagagatg aatcatcaac caaatgatga
      421 attaatagaa tatattgatg aaatcctaca ggagttgatt acagggcatg gagattatgg
      481 tttagtaatc actgattgta atgaatacaa tgaggactta cttttattag aaaaaaagtg
      541 ggaagattta aatattgaaa ttaaaaaagt aagaatgaaa gaacagaata accagttatt
      601 aagtataagt gaagagtttt tttctcttgc aaatgataca gtgtttaaaa tagaaaactt
      661 ttctaaagaa aaaagtaatt acttaatgac tttaataata attatatcta ttataggtat
      721 attagcatgt atcatattga ttttacaata tagtaaaaaa atggttagac tggaaaaatt
      781 aaatgtagat ttaaaaaata ttgcatataa agatgaacta acaggagtaa atactataga
      841 aaaatttaaa ttggatgcaa atcaaaatat ttgtatacat aaggataaga aatttgctgt
      901 attctatatt gattttgaaa actttaaata cattaatgat atatttggat atgattatgg
      961 tgacatgata ctgaaaagat atgctaatct tatgatgaat gacataggga aatatgaaat
     1021 atttgcaaga gaaattgcag atagatttgt tgcgttaaga tgctatgtag ataaggaaga
     1081 tttagtggta agacaacgag atgtagacaa tgaactaatt aatataacaa atgaaattaa
     1141 aaataaacat agtattactg ttgttagtgg aatttgttgt attgaagatg taaatgaaaa
     1201 attaagtata gatggtttaa taaatagagc aaattttgca caaaaaactg ttaagaataa
     1261 accaggtact aattatgctt tttataatga cagtattcgt aaaaaaatga ttgaagaaaa
     1321 tactatcaaa agtagaatac atgaagctat tgaaaaaaga gaatttatag tttatttaca
     1381 gcctaaagtt aatttacata gtcaaaaaat aaattgtgca gaagctttag ttcgttggct
     1441 tactccggat aaaggtatta tatctccagc aatatttatt ccagtacttg agaagaattt
     1501 ttttattgcc ttagtagata agtatgtttt tgaggaggtt tgtaaatgga ttagaaagag
     1561 gttagatgaa aataaacctt ttgttcagat atcagttaat gtttcaagaa tacaatttta
     1621 taacactaaa tttgtagaaa cttattccaa tatacaaaat aaatacagga tacctaaaaa
     1681 tactatagaa attgaattta cagagagtgt tgcttttgag aatcaaaaac atcttttgga
     1741 aattatacat gatttgcatg aaaatggatt tacttgttct ttagatgatt ttggaaaagg
     1801 ttattcgtct ttaagtgttt taaaagattt accatttgat gttttaaagc ttgattctat
     1861 gtttttcaag gctagtttag ataaagataa agagaaaata gtaataaaaa atattgtaca
     1921 tatgttaaaa gaacttaata tcacaactgt tgctgagggc attgaatgca aggaacaagt
     1981 tgaatttctt agggatattg ggtgtgattt agttcaggga tttgtatttt ataaaccaat
     2041 gccaatatta gaatttgaaa aaatattgga taaagagttt gtttataata gctaaaatgt
     2101 actttataaa atatgaacta attaagtaaa attattttgt actgaataaa tttttacttt
     2161 atttacagaa taatgattat ttacagaaaa gcgcgaaaag tgtattcctt taagtgtggg
     2221 atggatagca caaatagagg agaaaaaata ttggccatat aagaatgcat aataatataa
     2281 atatttaaaa ggtcagcatt atataaaaat taaaaagaaa ataaataatt tttctataaa
     2341 aaagagacta tgagaaaatt tactcatagt ctcctaaata atgtatttat tttcttcctg
     2401 caacccattt taatccccaa tgaaattcag tatccatttc tctatgacct ttgaagtatt
     2461 taactacttt ttcaacactg aatgtctcat catttacatt ttctatcaat gctattgaac
     2521 acatattata gccattttta tgttcatcaa gtttgacaac taagtctgaa tcttgttttt
     2581 gtttgattgt tacaattcca tcagcttcag accagttagc tacaccttcg tatataaaag
     2641 cataaataag tattcttttt atatctttta ttttattacc atttattctt aaattttctc
     2701 cttgtgtatt acttcctgtt ctgtcatctc catctaattg agcaaaaggt ggattgtcta
     2761 aactaccaaa ggcatttcct aatgcttgaa cagcaccttt tacaccattt ttcatctcaa
     2821 ataggcaacc caaatctaaa tctatattac tgcttcttaa agagccaaaa aaaccctttt
     2881 tttgtacttt ttgattccaa tttaaattaa ctaaaatttc acctaaatcg ctattttcct
     2941 tttttgtaag gtttattttt tgtccctttt ttaattctat agccatagta atcttcctcc
     3001 acaaacatag atttatttaa atggtagcac ttaataagtc aatattcaac aagattagaa
     3061 taataatatt aaaattatac taaatatact aaaaagtagg aatcacaata cctttgtatt
     3121 tttcttcaat taagcttctt gctttatctg atgtaagaac atcttgtaga actttaatct
     3181 tttcactgtc tttatcttca gttctaaatg ctaaaagatt tgcatactcg tcatttatag
     3241 gagctgtata aattccgttt ttcttggcat caagaccagc aggtacagca taagtagtat
     3301 ctataaatgc agcagtcaca tctggaagag aagatggaat ttgagcaata tctaactctt
     3361 tgaattgtat tccctttgga ttttctatta tgtccttagg actaactaat ccatcccctt
     3421 taggtaacgt taaaagtcca atactttcaa ggtatctaag gttcttagaa caagaagatg
     3481 ggttattgct tattgcaatt gtatctcctt ttttaaattc atcaacagat tttatcttat
     3541 aagagtaaag tattgctggg catacatata gttttttacc agccattatt ttgtaacctt
     3601 tggccttaac agcttctttt agataaggtt catgttgaaa tagatttgca tcaatttcac
     3661 cattatttaa agcttcgttt ggaagtatgt aatcatcaaa gtttttaact tctaaagtat
     3721 atcctttttc tttaataagt ggttttaatt cttctaataa ctctcctcct ggaactaaag
     3781 tagcaccaac aacaattttt ttatcatctt tagctttaga acatccaacc acagctaaag
     3841 ttagtactag acataataac aaacataata attttttgaa tttcatataa attccctcct
     3901 gaatataatt gattagttaa aaataaaata ccattcttta tcacactgca aaataaaaat
     3961 aagcctgagt ttaaactcag gctttaaaag ctaatttaga ctcatctttc agtataatac
     4021 tgcaggattt agcaccaatg tatataaaaa tatactggtt gctgggtttc atagggccag
     4081 tcccttcacc actctggata agaagtatca atttttaatt atgttataaa tattatcatg
     4141 attttttacg tctgtaaaca taattttata aatattttaa aatctttaat gtattttatt
     4201 taacacctaa aattccagaa actagaatat cagtatcagc acctaagaaa tagtcattta
     4261 tttcaataga atccagagta tttctcaaag cattttcttc atgccttata ccaataagtt
     4321 tattttctac aaatgcaaca tcacattttc caaaaaaatc tccaaaaaat tttatactag
     4381 ttattatgcc tttatcaaca tttaaattaa actcaacatt tccaccagaa tattttaatt
     4441 catttgaaag agtatatttt ggtgaattac caaaattcca atcccaagtt tcgtattttg
     4501 atttagtaag cttttctatt tgttttatat catgctcact caaacaataa ttagtattat
     4561 cagtatttgt tttagaaaga taatccatta tagcatcttt aaattctaat atgtccattt
     4621 ttgaatgtaa atgttcactt atattggtta cacgagattt tacagatttt attccttttc
     4681 cttcaaattt tataggttta acctttaatg cttttgagag gtcatttatt tcagatgaaa
     4741 ataaaagagt cccatgatgc attactttgt ttttgtagtt gtattgagca tttccagaaa
     4801 atttcttgcc atctataaga aggtcatttc ttccagaaaa ttcggcattg acatctagtg
     4861 ttttaagtaa atctattatg ggctgagtga atcttctgaa atcactaaaa ttattattgt
     4921 tacaagctat aaatgtaaaa tttatatttc ccaaatcatg aaatacagct ccaccaccag
     4981 actgtctcct aacgacaggt atagaatatt cttttatata ttctaaattt atttcagaaa
     5041 gtgtgttttg gttttttcca acaattatag aaggttcatt cctccataaa ataaataagt
     5101 cttcatcaaa attttttaga agatattctt ccattgctaa attaaaataa gggtttgttt
     5161 tttcattata aattaataac attaaagtag ttcctccata gaattgataa aatttttatt
     5221 atgaatacaa ttaattttat aattcgacaa taatataaaa actcctttaa attagaaatt
     5281 ttaaaaacta ccttgcaaag tttaaataaa taatatatac tcaaaatatg tttaataaaa
     5341 gtgaatagaa gggtagaggc gcaataatct atcagtacta ataaggagtt aagcacagag
     5401 agttattagg aaaggagata ttgccgaagt tttataatcg atgctttaag attatattgc
     5461 tgggggtaca tagaaaatat gtacaactgt cacaggttta tttgtggtga gctatcattt
     5521 aaggaattat attatattta tttttactat tgttgatata ttgtaatgtt taagccttga
     5581 gtgtttacca ctcaaggctt ttttagtttt attgtaacat aataccctgt acagttcaca
     5641 aaattaaaag gagagggaga tttatatgag gaacaaaaaa attaatatta tttttctgac
     5701 gacaatcatg ttcataatgt caacagtaat ggtttttgca gaagaagata tagacactat
     5761 tgcactagcc aatgcagaaa aatttgggat tttaacttta ataccaccat tagttgctat
     5821 aatactcgca tttataacta aaaacgtaat aatttcttta cttataggta ttctttctgg
     5881 tagttttata attaaagcta gtggtattaa tgtttttgcg acatttatac aggcattttt
     5941 agatttagta gatagagctt tagtatcttt agcagaccct tggaatgcgg ggataatact
     6001 acaggttttg tcaattggag gagtaataaa tcttgttgct aaaatgggag gagctaaagc
     6061 tatagctgaa gcacttgcta aaagagcaaa gtcagcaaaa gggacacagc ttataacatg
     6121 gtttttgggg cttttagtat tctttgatga ttatgccaat tcactaatag taggtcctat
     6181 gatgagacct gttgcagata aaatgaaaat atcgagagaa aagctagcat ttataataga
     6241 tgccacagca gcaccagttg ctggtttagc tataatatct acatggatag gtcttgaggt
     6301 tggtttaata catgatgctt ttgaaagtat aagtatagat gtagatgcat ttggtatatt
     6361 tttaaataca ataccattta gattttacaa tatattaata ttagctttca ttgtaatatc
     6421 agcattatta ttaaaagaat ttggtccaat gagaaaagct gagataaaat ctagaagtag
     6481 aaaaattagt atagaccttg atgaaggcgt agaagagttg gatgatttag ctccaaaaaa
     6541 tggagtaaaa ttgagtgtat ggaatgctat aatcccaata ggaacattga taatagttgc
     6601 attagcatca ttttattata gtggatatac atctataatg ggaggagacg ataaggctct
     6661 gatacaacta tttacaaatt ctccatattc ttttgaagca ataaaagaag catttagtgc
     6721 atctgatgca tctagagcat tattccagtc tgcacttgtt gcaagtttag ttgctattat
     6781 aatggctgta gtaaaaaaga tttttacaat atcagaagca attgatgttt ggatagatgg
     6841 aatgaaaagt ttagtaatca ctggtgtaat attaatactt gcatggtcat taagttcagt
     6901 aataaaagag ttaggtacag ctaaattttt aatacaccta ctatcaggtt cactaccacc
     6961 attcttatta ccaagtttaa tatttggttt aggagcaata atatcttttg ctactggaac
     7021 agcttatgga acaatgggta tacttatgcc acttgcaatt ccattagcat actcattaaa
     7081 tcctgatatg tcttatgtaa tagtaagtac tagtgcagtt ttaacaggtg caatatttgg
     7141 agaccattgt tctccaattt cagatacaac aatactttct tcaatgggag ctggctgtaa
     7201 tcatatagac catgttaaca ctcaaatgcc atatgctata tttacagcag taatcactat
     7261 tgtatttggg tatataccag ctggacttgg attgccaata tatatagtat taccagtggc
     7321 aatagctgct atatttgtag gtattcaaat aattggtaag aaagttgatg aagcggaaat
     7381 agaacttgta gaataaagat ataaataagt ttcacctttt attatcacta ttttgttagt
     7441 ttttagataa ttttaaaaag ttattgaaaa taatggcgaa atatagtaaa ataaaagagt
     7501 gataagcgga tgtggcggaa ttggcagacg cactagactt aggatctagc gccattggcg
     7561 tgggggttcg actcccttca tccgcacttt acagtactta attgtactga aaaaaactgg
     7621 ggttgctggc tttcggagtg ggctatcccg tttttatttt tttgtatata taatagattg
     7681 gcactttagt ataaggtgct ttttttatag gcaaaaacag tacatttatt tctgttttat
     7741 tatcattcat ctattagaat atactttagt taagaatgtg ttaataagca gtgttcacat
     7801 tcttgataaa atgtggaata atatgtattt ttactaagac ggaaatgaaa taattaaacc
     7861 attaaataat attataagat gatgaataag tagataaatc aaattttgta ggaggatatt
     7921 atggaaaaat atatatggtt gtttccatta ctttttattt ttcatgatat ggaagagata
     7981 ataggttttg gaatatggct taaaaaaaat aaatcaatgc ttgataagaa gtatccattt
     8041 atcagtaaag tttatgaaaa ttatagtact gaaggcatgg catttgctgt gtttgaagaa
     8101 tttattttat gcattatttt ctgtatactt acagttataa cagagaatca gtatgtatat
     8161 ctactatggt taggtagttt tatagcatat accttacatt tggtaattca tatagggcag
     8221 tcaatcataa taagaaaata tataccttca cttattacca gtatcatatg cttaccaata
     8281 agcatttggt gtataagtaa gagtatttat atagttgatt gtgaaatgag tacaacaatt
     8341 ctgtacagta taattggcat tataattgta gcattgaatt taaagtttgc acagtcgctg
     8401 attggaaaat ttacaaagtg gatgtcaaac atataaattt tagtttattg gacagttcaa
     8461 ggaatcagaa attggaattt tatagtatta atattttatg gtattaatat aatgtataaa
     8521 attttatagc aatgtatttc ataagtataa agtgactaga attttaagaa atatacaaat
     8581 tctataaaaa ctaaataatt tcaagtatat tttaaaaatc ttatttttca caagtttaaa
     8641 attgtaaacg atttcatcaa gtctatgaat atacttttaa catgttatag taaatctcaa
     8701 tataaattta catttggttc tttgtgtgtt attttaataa taaattaagt gaaaatgtta
     8761 ttttacatta atatataata tctttgcata agagtgaata atataaaaga gaaataattg
     8821 ataataaaaa cttaacaaaa aaattaatac ataaaaaaaa ataaatataa tgtgttgaca
     8881 tttaattgaa atattcatac aataaaattg aaaataaata tttccaaatg agattagcga
     8941 gagagtcctt tatacgaaaa ggcgccgaag aagaaattta agaaatgcca atttttagag
     9001 aagctttcag gcaaaaatat cgctaattga tgggactctg gagagactta agttaagata
     9061 ctataagtca ccgaagaagc aaaataatat taaattatta aatctttcag gtatacaaac
     9121 agggatatat aggctaggat tattttccat gctgatatat ctttttttta tgaaaaagta
     9181 caggaaataa tatcaaagtt acttaaatac agtaggtcta attttctagg gagggttata
     9241 tatgaatgaa ttgaaaagag tatctttgta caatatacac aaagagcttg gagcaaaact
     9301 tgttgaattt gctggttggg aaatgccttt agaatatgag ggtataaata aagagcatga
     9361 gaaggtcaga aaaagtgcag gtatatttga tgtatctcat atgggagagg tacagataaa
     9421 gggagcagag tcagaaaaat ttatacaaaa cctagttaca aatgatataa gcgcattaaa
     9481 gataaatgat attatatata cacctatgtg ttatgaaaat ggtggagtag ttgatgattt
     9541 attaatatat aaatttggtg aagaagatta tttattagta ataaatgcag gtaatataga
     9601 taaagatgta gcatggataa taaaacaaag tgaaggatac aatgtagata taaaaaatat
     9661 atctagtgaa gtgagtcaat tagcaattca aggacctaaa gcagaagaaa ttcttcaaaa
     9721 aatcacagat atagacttaa actcaataaa attttataag tcaattccat caacaaaagt
     9781 atgtggttgt ccttgtttag tttcaagaac aggatatact ggggaagatg ggtttgagat
     9841 atactgtaaa aataaatatg tagaaataat ttggaatgaa gttctaaagg ttggaggaga
     9901 agatatatgt ccagctggtc taggttgtag ggatactttg agatttgaag cagctctacc
     9961 tctatatgga catgaaataa atgaacacat atctccaatt gaaggtggtt tatctatctt
    10021 tgtgaaaaca aataaagaat catttattgg caaatcaatt ttatcaaaag aaaaagaaag
    10081 tggagctaaa agaaagttag taggatttga aatgcaaggt aaaggaatgc ctagaaatgg
    10141 atatgatata aggattggtg ataaaacagt tggatttgta acaacaggat gtgcatctcc
    10201 aacgacagga aaaattttag gtatgggaat tatagattct gaatatgcaa aagtaggaaa
    10261 tgaaataggt atagcaatca gaaaaaaagt tgttcctgct gtgatagtta agaaaccttt
    10321 ttataaaaaa caatataaaa aagataatat tatattaaac aaagaaaata aattttcata
    10381 tatacctgct acaagtgaag ataaatcaaa aatgttaaaa gtagttggat taaattcagt
    10441 tgatgaatta ttttcagata taccagaaga agtaaaatta aaaagagatt taaatttgga
    10501 aattggaaaa tctgaattag aggtaagcaa aatagtaaaa agattgtcag aagaaaattt
    10561 aagtttagaa gacttgactt gttttcttgg tgctggtgca tatgaccatt atataccatc
    10621 aattataaaa catataactt caagatctga attttataca gcatataccc catatcaagc
    10681 tgaaataagt cagggaactt tacaagtagt ttttgaattt caatctatga ttgcagaaat
    10741 tacaggtatg gaaattgcaa atgcatcaat gtatgatgga gcaactgctg caatagaagc
    10801 atgtataatg gcaatgaatc agactagaaa aagtaaaata gtagtgtcaa aaactattca
    10861 tcctgaaact ctgtcagtat taagaactta cttacaatat aaagattgtg agatagtaga
    10921 aattgacttt tgtaatgaat atgggactac agatatagaa aaattaaaag cttctgtaga
    10981 caaagataca gcatgtgttc ttatacaaac tcctaatttc tttggaatta ttgaagaaat
    11041 ggaagaaata gaaaagataa ctcatgaaaa taaggctatg ttaattatga gtgtagaccc
    11101 aatatcatta ggagttttaa aaacaccagg tgagataggt gcagatattg ttgttggaga
    11161 agctcaatca cttggaaacc ctcttaattt tggaggtcct tatgtaggat ttttagctag
    11221 caaatctaaa tatactagaa aaatgccagg aagaatagtt gggcaaagtt tagatgtaga
    11281 aggtaaaatt gcatatgtat taactttaca aactagagaa cagcatgtaa gaagagaaaa
    11341 agcaacttct aatatatgtt caaatcaagc tttaaatgct cttgtagctt caatatatat
    11401 ggctactatg ggtaaagaag gatttaaaga ggttggtatg cagtctatga aaaaagcaca
    11461 ttatacctac aataaacttg tacaaactgg aaaatataag ccaatattta aaggtaagtt
    11521 tttcaaagaa tttgctgtac aaggaaatct taatatagaa actataaatg ataaactctt
    11581 agaagaaaat atattaggag gttataattt agaatacaat tatccagaat taaaaaattc
    11641 aactttgctt tgtgtgactg agaaaagaag caaagaggaa atagataagt tagttggaat
    11701 aatggaggga ttataatgaa agaatacaat agtttgttga tagatatatc taaaaaaggt
    11761 aggaaagcat actcacttcc aaagttagat atagatgata taaaaataga aaatatgatt
    11821 gaccaagata tggcaagaca aagtgagttg aatttgccag aagttggtga attagaatta
    11881 gttagacatt atacattatt atcaaataaa aactttggag ttgatacagg tttttatcct
    11941 ttgggttctt gtactatgaa atataatcct aaaataaatg aagatatggc agcacttcca
    12001 aactttactg gaatgcatcc ataccaatct tcggatacag ctcaaggttc tctttctttg
    12061 atgtatgatt tatcaagaag acttgcagaa attacaggta tggatgaagt tacactacaa
    12121 ccttcagcag gttctcatgg tgagtttaca gggcttatga ttataaaagc atatcatgaa
    12181 aatagaggtg accataagag aaccaaagtg ataattccag attctgcaca tggaacaaat
    12241 ccagcaagtg cagctatggc aaactttgat gtgattcaaa tagcatctga taaaaatgga
    12301 gctgtagaca taaatgcatt aaaagaggtt ttgaatgatg aagtagcagc tcttatgctt
    12361 actaatccaa gtactcttgg tttatttgaa aaaaatataa aagaaatagc tactcttgta
    12421 catgaagcag gaggactttt gtactatgat ggtgcaaaca tgaacgctat tatggggata
    12481 acaagacctg gagatatggg gtttgatgtg gttcatctta atcttcataa gactttttca
    12541 actcctcatg gaggtggagg tccaggagct ggtcctgttg gagttaaaaa agaattagta
    12601 ccttttttac caataccagt aattgaaaga aaagaagata agtatgtact aaattatgat
    12661 agagaaaaat ctattgggaa aattaaaaat ttctatggta attttggagt tcttgtaaga
    12721 gcttacacat atatactaac tatgggaagg gatggactta aagaggctag tgaaatggca
    12781 gttttaaatg ctaattatat aaaggaaagt ataaaagatg attatatttt gccaatagat
    12841 accttgtgta agcatgagtt tgtattaggt ggattgccaa gaggagaggc caatataaaa
    12901 actatcgata ttgcaaaaag actactagat tatggatacc atcctccaac aatgtacttt
    12961 cctcttatta taaatgaggc acttatgata gaacctacag aaagtgagag tatagagaca
    13021 ttagatagtt ttatagaagc tatgaaaagt gttgctaaag aggcaaagga agaaccagaa
    13081 ctattgaaaa ctgctccaca taatactttg gtcaaaagag tagatgatgc aagggcagta
    13141 aagaaaccta tactaacatg gtcacaaaga taaatagtta aaaagtagag atttaaagta
    13201 taaagagaat taaatttgta agaaaaagct atctaactta atctagttta gatagctttt
    13261 ttgtatatta gtatgtatgg tattttctat ataaagtttt aaattatatt agtgcttata
    13321 tagaatattg atatgatgat tatgtataga tattaataag aattgttgaa tttatcttgc
    13381 cttaacttta tttactcttt tatattaaga aaaacttaac taaaatatag cgaaattcat
    13441 aaaaatttag gaaatcttaa gaattataat atttaaatct ttagatttat attttattat
    13501 ataaactata gaataataaa ataaaaaaat tgagaatttt ataatatatt tgtagatttg
    13561 agtataaaaa agtgctaagt tgaaaggagt aaaatatgga taaggttaat ttagaaaggt
    13621 atagttgtga tataaatgtt ggtttaaata aaaatcaact tcaaagtaga atagatgata
    13681 atttaattaa tgcatttgat aatggaaaaa ccaaaacata taaacaaatt tttaaagaca
    13741 atatatttac actttttaat ctcataaata ttgttttagt ctgtttatta gtttcagttg
    13801 gttcttttaa aaatacctta tttatattta ttgctgtaat taatactatt ataggagtaa
    13861 tacaagagat aagagctaaa agattattgg acaatctatc tgttatagtt tcaaataagg
    13921 tatctgtcat aagagaaaaa gagaaaaaag agattgatgt acaagaatta gtattagatg
    13981 atattatgat tttaaaaaca ggtaatcaaa tttgtgcaga ttcaaaagtt ttaaatggaa
    14041 aattagaggt aaatgagtct ttggttacag gtgaatctga tattattata aaaaataaag
    14101 gcgatttttt atattctgga agttttgtag tttctggaga agcttttgta agagttgaaa
    14161 atataggaaa agataactat gcaaataaga tagctaatga tgcaaagatt tctaaaaaac
    14221 ataattctca acttagaaat tcattaaatg caatattaaa aattgtaggt ataataataa
    14281 ttcctttagg aattatattg tttgcaaaac aacattttgg aaatggagat tctatagcta
    14341 catctgtagt aggtacagtg gcagctgtaa atggtatgat accagaggga ttaacattac
    14401 ttacgagcat tgctctagct gtaggaacta taaaacttgc tagtcataaa actttagtgc
    14461 aagaactgta ctgtgtagag actcttgcta gagttgatac actatgtctt gataagacag
    14521 gtacaattac agaaggtaaa atgcaagttg atgatatagt aatgttggaa gaagtggata
    14581 ttaatgaaat aatggggaat atatgtaatt ctctcaaaga tgataatgca acactaaatg
    14641 caataagaga taagtataat gttttagata cgtataaagc taagaattta ataccatttt
    14701 cttcagaaag aaaatacagt ggggttacct ttgaaaataa aggtacatat ttattaggtg
    14761 catttgaatt tatatttaag gaaaaaaata aaaaacttag gatagaagtt gaaagatatt
    14821 ctaaaaaagg aaacagagta atagttttag ctcatagtga ttcttatatg gaagataata
    14881 atattcctga agatataaag ccactagctt ttatattgat attggataaa attagagatg
    14941 aagctaagga aactttagaa tttttttaca atgaaggtgt agatataaaa attatatctg
    15001 gtgacaatcc tataacagta agtgaggtag ctagaaaagc aggtcttaaa acagctagta
    15061 attttattga tgcaacaaca cttaaaaatg atagtgatat atacaaagca gttgataaat
    15121 acagtgtttt tggaagggta agcccacaac agaaaaaaca aatgatttta gcattaaaaa
    15181 aacaaaatca tacagtagct atgactggag atggtgtaaa tgatgtgcta gcacttaaag
    15241 aagcggattg tagcattgcc atggcatctg gaagtgatgt tactaagaat gtgtctaatt
    15301 tagtcttatt agattcaaac tttgcatcaa tgccaaaggt tgttatggag ggaagaaaag
    15361 taattaataa cattcaaaga gcatcttctt tgtttttagt aaagacaatt ttttcattct
    15421 tcttgtcgtt attaacatta ttcttcttca gtagacagta tccatttgtt cctatacaat
    15481 tatcactcat tggggctgta acagtgggta taccatcatt cttcttagct cttgaagcaa
    15541 acaaaagtag aatttcagga aattttttac tcaatatatt aaaaaatgct ttaccaggag
    15601 cactatgtgt gacagtaaat gtaattattg tttacaatat agttgaatat ttaggttata
    15661 gttcaagtgt attttccaca atgtgtgcta tattaactgg tgtatgtggt cttttaatat
    15721 taagacaaca atgtttgcca tttaataaag aaagattatt tttaatagta agtatgacta
    15781 ttgcatttgt tattgggatt ctatttttag gtggattatt ctcatttgta gcattgagta
    15841 gagaattaat tctcataact atggcattag caatattaat gccaattatg ataaaagtta
    15901 tgttatttgt cttagatgag atattagaga caattgttcc agatttaaat taattttaaa
    15961 atgatataaa taagagcagt tttagttgaa ctgctctttt tagatgtgtt taattttatg
    16021 taagaatatg tatttttatt aatcagctat atatgagcaa cagtagtcaa caacttcatt
    16081 aacctttaag tcaaaacttg aatcactagg aacattaaat ttttgccctt ctttataagt
    16141 tacaaactca ttgcttccag gcagttttac atccatactt ccagcaagca tctccattat
    16201 ttcttcttct ctagtagaaa aagtgtattc tcctggcaac attaatccta aagtttttct
    16261 ttctccatta ggcagtatta ttactctgct acttacctta ccatcaaaat aaacgtttgc
    16321 cttctttact gcagtaacat tcttaaattc actcattaga taaaacctcc aaaattattt
    16381 gtacatatat attatataaa aataatgtaa aaaatgctat atatttacaa aaaaatttag
    16441 tatttattaa aaaaattaat aagaataaaa aagagatgaa attgcttaaa ttgataaaaa
    16501 atataaaata aattgcaaag taagcaagaa aagaataagt tgaagagtta tatattataa
    16561 atattaaaaa ttttagtatt aataaaaaag atattacatt aatatga
//
LOCUS       DAYATN010000081        16392 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.081, whole genome shotgun sequence.
ACCESSION   DAYATN010000081 DAYATN010000000
VERSION     DAYATN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.081"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(445..1317)
                     /locus_tag="ACGI8A_002423"
     CDS             complement(445..1317)
                     /locus_tag="ACGI8A_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0180417.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(1711..2289)
                     /locus_tag="ACGI8A_002424"
     CDS             complement(1711..2289)
                     /locus_tag="ACGI8A_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0180418.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(2347..3063)
                     /locus_tag="ACGI8A_002425"
     CDS             complement(2347..3063)
                     /locus_tag="ACGI8A_002425"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFH0180419.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            3254..4339
                     /locus_tag="ACGI8A_002426"
     CDS             3254..4339
                     /locus_tag="ACGI8A_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFH0180420.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(4494..5153)
                     /locus_tag="ACGI8A_002427"
     CDS             complement(4494..5153)
                     /locus_tag="ACGI8A_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0180421.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(5370..6662)
                     /gene="lysA"
                     /locus_tag="ACGI8A_002428"
     CDS             complement(5370..6662)
                     /gene="lysA"
                     /locus_tag="ACGI8A_002428"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFH0180422.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(6680..7873)
                     /locus_tag="ACGI8A_002429"
     CDS             complement(6680..7873)
                     /locus_tag="ACGI8A_002429"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFH0180423.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHLVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     regulatory      complement(7955..8123)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(8234..8851)
                     /locus_tag="ACGI8A_002430"
     CDS             complement(8234..8851)
                     /locus_tag="ACGI8A_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180424.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(9021..10721)
                     /locus_tag="ACGI8A_002431"
     CDS             complement(9021..10721)
                     /locus_tag="ACGI8A_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180425.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(11118..11681)
                     /locus_tag="ACGI8A_002432"
     CDS             complement(11118..11681)
                     /locus_tag="ACGI8A_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180426.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVYNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(11840..12553)
                     /locus_tag="ACGI8A_002433"
     CDS             complement(11840..12553)
                     /locus_tag="ACGI8A_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HFH0180427.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            12845..14509
                     /locus_tag="ACGI8A_002434"
     CDS             12845..14509
                     /locus_tag="ACGI8A_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFH0180428.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLKQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(14616..15515)
                     /locus_tag="ACGI8A_002435"
     CDS             complement(14616..15515)
                     /locus_tag="ACGI8A_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HFH0180429.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(15542..16180)
                     /locus_tag="ACGI8A_002436"
     CDS             complement(15542..16180)
                     /locus_tag="ACGI8A_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HFH0180430.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
BASE COUNT         5298 a         2713 c         1776 g         6605 t
ORIGIN      
        1 ccaaaaatgg ctacttaaaa aatcaagatt tcatacctaa ctactaattc tatgattttt
       61 aaatttttca acttctttgt caaagttaaa ataaaatact aaaagacaca tattatagtg
      121 aacaaaaaag atagaaatac atattttaat ataattttaa taagatatta tatacttaaa
      181 tacaaataaa attaatttta taattgttaa ttaaggaaat gatattgttt agagaaataa
      241 aaaaagcact ctccatgaat ggaaatgctt tctttaataa attagttgac gtagtaaaga
      301 tgtattcaga atacatcttt atgttttgag tctcgaaata tttatttgta ttatttaaac
      361 gaaaatttat tttttaagtg ataatcgagt atttactaat aaggctatga ttctcaattt
      421 catagcctta ttaatataca aactttactc atacacagaa tatcttttcc aaatttgctc
      481 taaatcatca aaatgctgtt taatatcttc tttttccttc atcccaacag atatagcaag
      541 ggcatttgaa atactaagag ctggaactag agaatctaca aatgaagcca tattactctt
      601 aactagaaga gtgttatctg caagtgatgc tactggcgca aataagctat ctgttaatga
      661 tattacatgg gctccttttt ctttagcata attgacaatt tgataagatt tcttagaata
      721 acgagggaaa ctaatagcaa ctattacatc ttcttcattt attcttacaa tctgctcaaa
      781 agcatctccc atatccattc taattatatg aacattgtca agtattatat ctaagtaaaa
      841 acctaaatat tgagctacta caaaagaact cctcattcca agtatatata tttttctcgc
      901 tcttaaaagt ttgttagaag cttctttaaa cgctctctcg tcaatttctt ctagtgtaga
      961 ccttatatta tctatatcac tttttaaaac tttgtttagt attgcaaaat catcggaata
     1021 atcatgtgcc atttcaactc tttgtactgt agttagttta ttttttatga gttcttgaag
     1081 tgcagcttgt aatttaggat atccactata acctaatgca tttgcaaatc ttacaactgt
     1141 agactcactt actcctacgg tttcacccaa tttacatgca gtcataaatg caactttatc
     1201 ataattattt aaaatgtatt gtgctataag tttttgaccc ttactcaatc tcgtatattg
     1261 agattgtata tttgatatca aatgtttact gtccttcatt tcatttgttt cttccatttt
     1321 tttctccttt tagttatata aactattttg ttttattaac actgtctttt tctctaaaaa
     1381 ttttattttt tatctcaatt tcatcttagt ttttatcata gcttcttaaa ctcataaaat
     1441 tctaactttt ctgtacctta tacttcacat agtattttcg attaaattta ttataccatc
     1501 tttccttgca ttttttaata tttatccaat atttctttta aatttttcaa tttttactca
     1561 tttaaaagtt atctatctta ttttgcatat ttagatgtat attttgtttt gagaataaac
     1621 tgttgtatac ttattattta aagtattttt aataggtcac actatatata ttaactctta
     1681 atacaatctt gtcaagtttt ttagtattta ttatgctttt ttcttttcaa ttaaaatcaa
     1741 ttgtggtggg ttattgactt gatttaaaaa cttagattct aaaacattaa attcatcttg
     1801 atttaaatta ttgacaaggt tatatatgca atctttttct tcttgacctc catcatgccc
     1861 tgtatatatg gcaatgctta ttactccatt ttcttttaac atttctaaac ttaactttat
     1921 agcctttaaa gttgtatctg gtttggttat aattgaatgt ttcgctcttg gaagataacc
     1981 caaattaaat aaaacacatg aaacttcttc ttttatatat tgattcattt tttcatgccc
     2041 atcaaatatc aattttactc tatctgtata tccttctttc tctagtttct ttcttgtaga
     2101 ctttattgct tcttcttgta tatcaaaagc atatacaaag cccttttctc caaccttctc
     2161 agctagatac cttgtatcat acccatttcc catagtagca tcaattacta tgtcaccttc
     2221 atttataata ttttctaaat aaaatttatt tatatctgta attttagtta aatactttgc
     2281 atatgccata atactatttc tccctactat taactttatt tcattttttc tcaatttatt
     2341 tttatcttat tttattgatt ttaaatattt tatttcatct tctgttaggt atctatattc
     2401 tccaactttg gtgtctttta atactatttt ccccacagaa atccttctta aatttaaaac
     2461 tggatgattt atagctttgc acatttttct tacttgtcta tttctaccct catgtatagt
     2521 tatttcacac actgaataat ttttttcttt attttcttta gttaccctta ttttggcagg
     2581 tgccgttgtg taatcttcta tttttagtcc tgtttcgaat tgtcttatct catcatcact
     2641 tataattccc ttcacacttg ccacatacat tttatcaatt tcatgctttg gatgggtaag
     2701 cttatatgtt aaatcgccat cattagtaag tattaataaa ccactagttt cataatctaa
     2761 tctccctatt ggatatactc tttcccttac atctttaagt atgtctatta cactgggtct
     2821 attgaattgg tcttttactg tagttatata tccctctggt ttatttaata ctatatatat
     2881 ataattttca cttggtttta cttttttatt atcaaattct actatgtctt tttcttcatc
     2941 aatattaaaa gataactctt ttactacact tccatttacc tttattcttc ctccaagaat
     3001 tatttcttct gcttttcttc ttgatgctat tccacaagaa gctatatact tattaattct
     3061 catacttaat ccttccttat cttaaattat gtgtgtttaa tatttttatc aatgttaatt
     3121 ttgctacatt attatactat actagacctt aattaaaaac aagcaaaaaa attatttaat
     3181 tttcctaaat aattgtaatt tcttttcaca tactattaat atgtaatagc tgactaaaca
     3241 ctattttaag gaagtgatta ttatttcaaa ggaaaatata aaatattatt taatagtcgt
     3301 gtttattgga attttatttt ttaaatttat taatactcca tcagacttta tatctagtat
     3361 tgaaggattc ttaaagtttt ttagcccatt tttattagct attttacttg ctttactttt
     3421 aaatccactt gtgatgcttt ttgagataaa attcaaagca catagacttt tagccatttt
     3481 tttatcttat atatttatag gttttatttt agcctttgcc attaggttat taattccatc
     3541 aatagctaat acattaaata gattgataaa tgaaatgcct atgtatactg attatataga
     3601 tagttttata gaaaaaaata tgtcaaacat atattttcta aaaactttaa ttcctcatat
     3661 acaacatagt ttagataatt tattaaaaga agcaagtaat tttgttggta aaattcctaa
     3721 aaacttctta atctatacac ttagtatatc ttctatgcta tttaatatga caatgggatt
     3781 tatactatct atatacatta tatatgacaa agaaaaaatt gcattgggat ttaagagatt
     3841 tctatattct tctactgcta gaaacaaggc agataacatt atagaatttt ttaggactac
     3901 tcatgatatt ttctatgatt atttacttgg tagaatatta gactctctta ttattggtat
     3961 aattgcattt ttaggatttc aatttgttat cagaatagaa aatgctttat ttttagcttc
     4021 aataatattc ttaggtaata taattcccta ttttggtcct tttattggtg caatccctcc
     4081 aatagccatg actatattat atagtccaca aaaaactata tgggtcattg catttctatt
     4141 tatattgcaa caattagatg gaaattttat agaaccaaaa gttatgggta atcaagttgg
     4201 tataggagct atatgggtaa tatctgctat tttggtggga caatctctat ttggtttcat
     4261 aggagttttt ctttcagtac caatagctgc tgttgtaaaa acttatgttg ataaatatat
     4321 agataatcgt ttacaataga ttatctctac tgtgacttta atttttagtt taaaatttaa
     4381 ctgctattta ttaaattatt tctcaaaaaa tatctcttag taagaaaaaa ttgctaagag
     4441 atatttttta taatatataa tttgttcatt aaatttatgc atatatttta tacttattga
     4501 ttatttgagt tttgagaatt tccaccacta tctgaaccac cagtagtatc ttgtggagtt
     4561 ggtgctggtg ttggattact attatttggc ttttcttgat tttgagaaga actgttatta
     4621 ttgtcagact tattactatc tgatttttta tcattgtttt tagaactaga tttattattt
     4681 ttactacttc cactataaga agatttcctt gcatctgatt caactctata cctatcttct
     4741 ccagccgtat cttttgcctt tttctttctt tgttcctctg cttcgttttg ttctttttct
     4801 aatttagtta actcttcttt ctttttagtt gcctcttctt taataacaga ggaaccattt
     4861 ttgattttct ctataagttt aagttccttg attgcaccct cataattttt taattcttca
     4921 aactccgtca tatttgacat tctcatagct tgcatataca tagctcttgc attctcatta
     4981 gtactatctt cttccagtgc agaggataat gattccatag cttttttata gttatgtttt
     5041 tctaaatatg tttttccttg ctctactaaa tttatttcat cttttttatt aaatgaacat
     5101 cctacaagaa atatagataa tatcgatatt aataaaatta ctcttcctct cattaaatcc
     5161 ctccttgtca tatactttaa attttatcat aattataaag tttttaaaat attttatatt
     5221 tattacaaga aatttgttag taaaacaaaa aataagcaga tatgctacga acgatacaat
     5281 aaaccaaaaa ttaatttaga gatacatatc ctagcaaatc tgctcataat aaactttaat
     5341 atttaatttt tattatatac ttctctattt tatatctcta aagctaataa gtcctcatag
     5401 ctttgtctct tacaaattat cttatcattt ccatcaaata ctgataccac tgctgctctt
     5461 ggtattttat tataatttga agacattgaa tatccatatg caccagttga tgttgttata
     5521 agtatatctc cacttttaat atccattatg tttatatctc caattaatat atctccactc
     5581 tcacaacatt ttccagctat tgttacatga ttcatagcct catgattaat tttattcaca
     5641 atactacact cataactagc ttggtacaac gaaggtctta cattatctgt catacctcca
     5701 tctacactta cataaaccct aacatctttt atatctttta tagaacctac tgtatacaat
     5761 gtacttcctg catttgcaac tattgacctt cctggttcta ttaccaatgt aggtacttct
     5821 attccaagct ctttacatga agtttctgct ttagtgatta tggtttcaca aaactctttt
     5881 atagttttag gtttatctcc ttcgctataa taaacgccaa ctcctccacc taggtcaatt
     5941 tctgttagtt gtatgttgaa tttttctttt atttctttta ccaagttcat cattatatcc
     6001 actgtatcta tgtatggttc tacatcaaat atttgtgaac ctatatgagc gtgaagacct
     6061 acaagtttaa tatttttata ttcacttaat ttttctattg ctctaaataa atctccattt
     6121 actaaagcaa atccaaattt agaatctatc tgtccagttt ttatataatc atgtgtatga
     6181 gcttctatac caggagttat tctaaagtat atctcttgag ttttaccttt atcttcacat
     6241 aatgcttcaa gcaaatctaa ttcataaaaa ttatccacta caaatttacc tacacctaaa
     6301 tctaatccca tgtttatttc ttctttagtc ttattgtttc catgaaagaa aactttttcc
     6361 ataggaaaat ttgatttata agctgtatat aactcgcctc ctgaaactac atctaaacac
     6421 atttgttctt catttataag attacacatg taaagaggta aaaaagcttt ccctgcataa
     6481 gcaaccttat tttttccctc tctagcctta aaatattcta tatattctct acaattttgt
     6541 ctaactaaag cttcatcaaa tacataaagt ggagtttgat attttttact aagttctaaa
     6601 caactcactc ctccaatata taattcatca ttatttacag tcatagttcc atgaagtttc
     6661 ataatagcct ccccaaattt tatatattaa atacttctgc tattttttct atagctactt
     6721 gcttatcatc agaatttata gcacaagtta tacgaatctc tgatgtagtt ataagtttta
     6781 tcattatacc attttcattg aatatcttaa atactttagc agcaactcca gaagtatttt
     6841 tcataccaag acctactaaa gaaaatttag tgatattatt atcaatcact aaatgttctt
     6901 catctgtata tttagatact atctttttac attctcccaa ctcttctttt ggaactgtaa
     6961 atgatacact tatcttatct aaaataggtg cagtttggct tatcatatct acacttatac
     7021 ctatagttgc tatatcttca aataagctag atatattttc agctttaaaa tcttttattg
     7081 ttatagaact gtcatcatca ctagtagcta atcctgttat tactttatcc tctaatttca
     7141 tgtcttttcc ccctcttata tatgttcctt tttctgtacc acatgtacgt ccgacataga
     7201 tttcaacacc atatttttgt gcaagctcta tacttcttga atgcattact tgtgctccta
     7261 aactcgctaa ctcaagcatt tcttcatatt caatctcatc aagtttacta gcctttgaat
     7321 attttcttgg gtctgtaaaa taaattccat caacatctgt atatatctca cattttccat
     7381 ttaatttaac cgcaagagct acagcagatg tatctgaacc acctcttcca agagtggtta
     7441 catcaccatc ttcgttgatt ccctgaaatc cagttactat cacgacattt ccttcatcta
     7501 aactattttt aattctgttt acgtttatat catcaatttg agactttcca tgaagaccac
     7561 tcgtttttat atttaactgg taagcattat aacttatagc tttacatcca agtgcattta
     7621 atgctattga taataaagat gctgaaatca tttcaccagt agacattaaa gcatctaatt
     7681 ccctcttact aggttcatta ctcaattcct ttgctaaagt tatatattca tcagtggatt
     7741 ttcccatggc agatacaact attaccattt gtggattttc ttttcttcgt tctattatat
     7801 tttctgctat tgattttatt ttttcagtat ctgctactga acttccacca tatttttgaa
     7861 caattatact catgctttct ccccctcaag ctaaattttg ataaataaaa acgcccaaag
     7921 gtatactttg gacgtgtaaa tttcatagac caagtaacct tgtagctcac cactttaaaa
     7981 gtgacagcct tatgcatatt ctgcataagt ccagaaaatt tgtccgtcaa caaaatttct
     8041 tcggctatat accctttcat caattatcaa cgcttaccag ctcctattga atactattga
     8101 atacagctac ctctacccta ggctcattat ttttaattaa tttattaata tagttttatc
     8161 atattctata taatattgca acaattattt tacaaaatgt ggcaaatcga tagttttctt
     8221 ttctaaagtt agcttatata caattaaata gtttatttaa tcctaaatct ataaaattta
     8281 tgctatactc attatcatat ctctctaaat catttaattc ggatagatta tctggtgtaa
     8341 ctaccaaaac gtttggtact ttaaaaaatg gaattgtaga tttatctatt tttttagaaa
     8401 gattttcgta tttagaaatg tctatttcat tatttaagtc aacatcaact aaaacttgct
     8461 ttatattacc aaattggtct gtatattcaa taaataaatc gtatttcaca tcatctacac
     8521 taacatttcg gtaaaatctt tttacatcac aacctgcagt attgattcta acaactaatt
     8581 ctgatcctac tagagccttt attaaatcat ctcccttaaa aagtttacta ccgtttaaat
     8641 agtatacata agagaggtct ttataattat aataatccac atcacaagga tattttttta
     8701 gtgtatattc actacacatt tttctcaaag tttttttaaa atttttttta ttgtatcttt
     8761 tttggtctcc ataaattttt ctaaattgag actctgtaac acatctaacc ctgctaatta
     8821 aaagtaatat ctctttttcc atattcgtca tagtacattc tccttgaagt attatttgca
     8881 aagttgtact aatattatat taattttatg acattaaatt tacaaagaat taaaataaaa
     8941 tagctaattt ttatagaatt aaattctata aaaattagct atcactatgt taaatactat
     9001 tatcaatctt tttatttaaa ttatacatta ccttgatttt cagaatcttt ggaatctgta
     9061 ttttcatcag ttcctgtttt ttcttttgct tttttatctt caaagtttgc aatatttagg
     9121 ttatcaaatt ctgacatttc tattacacca gattcttctt ttgaatacga tattttgact
     9181 ttatcaccaa cctgagtcaa tggcaattcg ctagatactt ttgaagtagc cctaaatatt
     9241 atatctttat ttgaatctaa agttacataa taattagtgt taccacctcg cacatctggg
     9301 cttattcttg tcactgtgcc ttctaatacc tcatctttta aatcattatc aagttttaaa
     9361 tcattaccct tagattctaa tgcatcctta tagtttctta atgcttcatt cttatcttca
     9421 cctattccaa gtacattata atcttcaaca gatacaaatg caactctctt aacaagaccc
     9481 gccttatctt ttaatgatga tacatatgtt ggctgaccca atatattata cattacaggg
     9541 aacgtcgctt cataattttt ttcttgaaca ttaccttcag ctgatttcat tgcagccgtt
     9601 tcagtagctc ctggttgttt atataatcta gcttcttttg ttctagaatc aactaacata
     9661 aatccaattg tagattcatc tccacctgaa gatgttatac ctgtatacca ataagacctg
     9721 ttatcattgc cataaactaa tgatgtacct tctgttggta ctaaaactcc cttttctgat
     9781 ataacagagt ttaaaaatcc attaacataa acaccccagt ctttaatttg gtctgttaca
     9841 aagctttgtg gttgtattct atccacccat ttaggcgtgt ttttaacatc ataaacattt
     9901 atttttccag tttctgcatc aacagtggct attcctgttg catttgctcc tccatagcca
     9961 attttatgtt catataaact tacaacccaa taaggtcttc cttcatcatt tatttcaaga
    10021 gtgaaatctg tcataccaac atttacaata ccatgtatgt ataaatgtct ttgtaaatcc
    10081 tgatgtaagt aggcttctgg ttggtaaact atctttattg gctttccatc tatttcttga
    10141 actaattgga cgtcttgagg attactagct gatactttta cataaccact tgtaccactt
    10201 agagatgtta tccatttaat tatgtctctg tgaacaagag gcgctaccca gtacaactct
    10261 ccatctacat tttgtatatg aaattttcct aatttggata cactccctat tgcaggaact
    10321 tctccaagct ttttgtcacc taatttcatt gccatatctt catctacaag acgaatgtca
    10381 tttacactga cagggctaat gtcatcagta aatttacttt cagttacttt tcccaatagt
    10441 tctctatatg attttgcatg aagtacaggt gtagaagtta taaagggaac tactacaata
    10501 tacacaagca aagctattgc tattgaaaaa ttatatttag ccattttaga tgctctttca
    10561 tttctatcta acatcatatc tagtattcct gctacaacaa agaaaattat tcctactgaa
    10621 aataaagatg taaaatctaa tcttagtaca ggtaatttaa taaatgcata aattattaca
    10681 attattaatg caactccata tgttttaaga tatcgtttca tttaatactc ctctctaaaa
    10741 aatgttatta ttactattat atccatatat ttacaaaaaa taaatattta tcatatttat
    10801 tatatattaa gatatcttta tatatttata gttctgctta ttcaactata tttaacatta
    10861 tacgtgtatg tataaatact ttttatacac tgtttgtgct atccatcccg cacctaaagg
    10921 agtgggattt ttgcactatt ctgtattata tatatccaaa aatgttataa aaatacaaat
    10981 attagcagaa atttaaaaac tgaagaaagt attctaacta agatttacaa attcttaatc
    11041 ctatcgaagt taaactacta tcttcagcct aattatctag caatattttt ttatatgaaa
    11101 aactctaaat caagttttta aatgtttgcg atttttgata cataagaatg ttgtctagaa
    11161 ttatattctg ataataaacc ttcatactct aaggttaagc cagataaatt ataaaaatca
    11221 tttggcgtta cttcaaatgc ttgtttattt aaaatgtcta tctcactttt ttctaacatg
    11281 tcagcaacaa aatttgaaca tacatattta tattcagatt gtctctgaat attcattagt
    11341 aaatatatta atgccattgt atcgtaatag taatcttctc tataatcact aattaatttt
    11401 atatttttat atagattttc atattgtaaa ttatatactt ctaacgaata taccctacac
    11461 acagtgtttt ttcttatagc atataatcct tgatttatat tttcttcaac aaatcctcca
    11521 ataaatggat tccttggatt tagccttcca aaagaataca ttggttttag attttcattt
    11581 aatgatattg acacatgtgt atacagcttt ttacttatat tccttattaa gtaagataat
    11641 atagttcctg tatatgttgt tacaatatag actttattca tcatttcccc ccaatctagt
    11701 aactaaataa tattatacac ttacaattta atatcataag atattctaac ataataaatg
    11761 tggttttaca aattttgatt ttaaggtact ttaatattat ataatataat aaaaatataa
    11821 atataagttt tctatatttt taaccaacag atgttttctt caaattaatc agatgattta
    11881 gtatatcttg aacctttcca gagtccttta aatatcctaa tgccccattt atttgtgatt
    11941 ttaaatctat ttctacttcc atatccaaat caacatttcc tttttttata gatatatttt
    12001 taagtgttat tgggtaaaga taactttttt ccaaagtaat cttatttcca ttaacctcaa
    12061 atggaacttt tttattgtag ttacttaata ttttttctag cattttatca cttagtttaa
    12121 attttccaat tttcacattc tcaagactta caactaagtt tccatccttt aaagtaggtc
    12181 tcaattcaag ttcatattga ctatttacca aaccaaatac cttatatggt cctgctacct
    12241 ttattttacc atttttcatt tcaacaaaat tattttcaag ttcattaatt ccatgcttct
    12301 gcatcaatgt atatattaaa tttttaaact cttcctctga aacagatatt tttccaacca
    12361 ttctaagtgg attttttact acctccatag aatttatata tgagcctttt gataaatatt
    12421 cttcatctat gattttatta ttttgtgaac taacatcata tctttctttt ggtgtcaaga
    12481 tatatacaac aattcctgca agtactaaaa caataacaat taaacttaac aaaatctttg
    12541 caacctttcc cataatcgtc tcctttacaa atctgtcatc agatttaaaa taaatttaga
    12601 taactaactt cacataatat tcttaacaat atacatacta taatacagtc ttttaaaaat
    12661 taattcaacc aatctaatgt tttgtaaata aactgtaaaa acatgttttt agtataaaag
    12721 ttaatatttt gcataatact cttaattaca cctttatttt tttctaatct tggattaaaa
    12781 ttaatattat gcaatacagt gtagtgttct gtattataat caaattaaat aggaggtata
    12841 gaatatgtca aatgaaacga actcatctaa ctttataaag aatattatta taaacgactt
    12901 agaaacagga aaacatgaca gtatcataac tcgttttcca cctgagccaa acggatattt
    12961 acatattggt catgcaaaaa gtatatgtct taattttgga ttagctaagg agtttaatgg
    13021 aaaagctaat ttaagatttg atgatacaaa cccattaaaa gaggatgttg aatatgtaga
    13081 gtccataaaa gaagatgtaa agtggttagg atttgattgg aacgagttaa attttgcatc
    13141 taactacttt gatgaaatgt ataagagagc tttaatacta ataaaaaagg gtaaagctta
    13201 tgtatgtgat ttgacacaag aggaaatgag agaatatcgt ggtactctaa ctgaaccagg
    13261 aaaagaaagt cctcatagaa acagaactat tgaagaaaac cttgatttat ttgaaagaat
    13321 gaaaaatggt gaatttaaag atggtgaaaa aacattaaga gccaaaatag atatgtcttc
    13381 tccaaatata aaccttagag acccaattat atatagaata tctcattcaa cacaccataa
    13441 tacaggagat aaatggtgca tatatcctat gtatgctttt gctcatccaa ttgaagatgc
    13501 aattgaagga ataactcact ctatatgtac tttagagttt gaagaccaaa gacctcttta
    13561 tgattggttt gttaaagaat gtgagatgga aaatatacct agacaaatag aatttgctag
    13621 acttaatata aataatactg tcatgagtaa aaggaaatta aaacaacttg tagatgaggg
    13681 tattgttgat ggttgggatg accctcgtgt tcctacaata tccggaatca gacgtaaggg
    13741 atatactgca gaagcattgc gcaatttctg tagtgaaata ggggtatcaa aagttaactc
    13801 tacggtagat agtcaaatgc ttgattattt cctaagagaa aacttacaac caaaggctcc
    13861 tcttactatg ggagttttaa gacctttaaa acttattata actaattacc cagaagataa
    13921 gattgagatg cttgaaatag agaataatgc taaagatgaa tctcaaggaa agagattagt
    13981 tcctttctct agagaattgt atatagaaca agatgatttt atggaagaac ctgttaagaa
    14041 atattttaga tttttcccag gaaatgaagt ccgtttaaaa ggtgcttatt ttgtaaaatg
    14101 tactgatgta attaaggatg aaaatggaaa tgtagttgaa atacatggta cttatgaccc
    14161 tgaaactaag agtggctctg gctttacagg acgtaaggta aaatctacaa tacattgggt
    14221 tgatgctaaa tcagctatac catgtgaatt tagattattt gaaccattaa ttcttgatga
    14281 tatacctgaa aatgaaggta aacatttctt aaaacaaata aatcctaatt cattggaaat
    14341 attacaaggt tttgttgaac ctacacagat aaaagatgct aaaccttttg ataaattcca
    14401 atttgttaga aatggattct ttagtataga taataaatat acaactgatg aaaaatttgt
    14461 atttaataga atagttcctc taaaaagttc ttttaaacca ggtaaataat cttacaatca
    14521 aaaaatgaag acttaaaata taaattactt ctactataaa taaacttagt tagaagtaat
    14581 tttttatttg agtcttcatc atttacttta aatgattaac ttttttcact acagattccc
    14641 atgacttcta ttccatcttt tatgcattct atgttaagag gaaaatctgg tcctttttct
    14701 ccgtcaatat ctgttataag tgaatcttca cattctatat gcaccttttt agttttaaag
    14761 tatagtattt catttccatt atattgatct aaataatcac ctctaagcac acttatcaaa
    14821 actggtattg attttggtat tggcattcct ttaaaaataa ttacatccaa atatccatca
    14881 tctatttcag ccttatatgc tagatttata tttcctgctg ttttgccatt aaaaatcaac
    14941 ataagataca tatctccatc ataatacatt tcctcagaag atacttttat cttaaatttc
    15001 ctcatataca atgcttcttc tattcccttt atataataag aaattcttcc aaatgaattt
    15061 tttaagtctg tgtttatcct ttgtgataca tccgtaaaca taccagcact tgcaacattt
    15121 ataaagtact tattattgac tttccctatg tctattttct ttggttttga atttatgatt
    15181 tcatttatag cttcttttat atcaaatgaa atttgaagag catttgaaaa atcatttgca
    15241 gttcctgtag gcaaaattcc aattggaatt tttatgtcta attctttcat tgcatttact
    15301 actaaatcta cagtaccatc accaccagct atcaatatgt gttcataaga actatcaact
    15361 tccttaaatg cattctttac aggttcttta atagttaatc tatagggtat caataaataa
    15421 tttttttctt gatataattc tatgatagtg tctaaatttg ataatatctt tttttcacct
    15481 gaattaggat tgtatataag ttttactttt ttcattattt ctccttactc attttattct
    15541 tttagtatcc aaaatcttca tttaaaaaaa tcacatgtat tctttctggt gttaattgtc
    15601 tttttataag tgtataacta cagcatattc ttcctggact tgaacagtct atacatatac
    15661 cagtagtctt acatccagta tttgtactta atcttttaca attggctgga gcagctattg
    15721 tttttgttct ttttatagct tcatctacat ctttaactat tttatttact cctgctataa
    15781 ctattacttt atcaggtcca tatatcatag cagcaactct attcccattc ccatctaaat
    15841 tgtaaagttc tccatcctct gttatcgcat tagtacttgt aaaataagca tctgcagaaa
    15901 atgactttct atatatttct ttcatttctt ctggtttgat atcttttgca tatctatcaa
    15961 gaaaattata tttttctctt ataaaatcaa tcactcctgt ttcaaataag gtcatagaac
    16021 ctccacatcc tactagagaa cctgctttaa ctatttcttc aattttttta agaagctctt
    16081 ctttgttact tactaaatac cctttcatat tattgttttc caaagatttt attgtttttt
    16141 ctattctttt ttcattaagc caatttaaat tttgatccat tttaacttcc cccttatttt
    16201 taatatgcac ttaattattt ataaacttta ttttctttat aagtttattt tagtacatct
    16261 aaaaaaaaat acaatataaa acttaaatag ttttacataa ttttttccaa aataacttac
    16321 tcaatcaaaa aaggaaagta gatgtactat tctactttcc ctatattgta tgtagtattt
    16381 attgcctaaa at
//
LOCUS       DAYATN010000082        16252 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.082, whole genome shotgun sequence.
ACCESSION   DAYATN010000082 DAYATN010000000
VERSION     DAYATN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.082"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            236..1684
                     /locus_tag="ACGI8A_002437"
     CDS             236..1684
                     /locus_tag="ACGI8A_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFH0180431.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKN
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            1710..4100
                     /locus_tag="ACGI8A_002438"
     CDS             1710..4100
                     /locus_tag="ACGI8A_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFH0180432.1"
                     /translation="MSLIFTTKDQLVELGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGVRTYFEATNDEEFMMQNGLEILIETSRFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVFVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            4102..4773
                     /gene="pgmB"
                     /locus_tag="ACGI8A_002439"
     CDS             4102..4773
                     /gene="pgmB"
                     /locus_tag="ACGI8A_002439"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFH0180433.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     INREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGVKDEFDCIVNPSLLKRGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            4812..6182
                     /locus_tag="ACGI8A_002440"
     CDS             4812..6182
                     /locus_tag="ACGI8A_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0180434.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIENFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            6529..7371
                     /gene="licT"
                     /locus_tag="ACGI8A_002441"
     CDS             6529..7371
                     /gene="licT"
                     /locus_tag="ACGI8A_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFH0180435.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            7516..9444
                     /locus_tag="ACGI8A_002442"
     CDS             7516..9444
                     /locus_tag="ACGI8A_002442"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFH0180436.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            9505..10932
                     /locus_tag="ACGI8A_002443"
     CDS             9505..10932
                     /locus_tag="ACGI8A_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFH0180437.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFVVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            11343..12119
                     /locus_tag="ACGI8A_002444"
     CDS             11343..12119
                     /locus_tag="ACGI8A_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0180438.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            12159..12500
                     /locus_tag="ACGI8A_002445"
     CDS             12159..12500
                     /locus_tag="ACGI8A_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFH0180439.1"
                     /translation="MQEITSKFKECRKAISAMGDETRQLILIALLESDFNGIRVGEIT
                     KKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWRSLTELINLIFVGIQ
                     HISENDIRKGD"
     gene            complement(12676..13425)
                     /locus_tag="ACGI8A_002446"
     CDS             complement(12676..13425)
                     /locus_tag="ACGI8A_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFH0180440.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELIST
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFEIITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            13647..13775
                     /locus_tag="ACGI8A_002447"
     CDS             13647..13775
                     /locus_tag="ACGI8A_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180441.1"
                     /translation="MKDYKYKGYFEGVEIFDNVNDLNLNTLTTVSRFVFVFLFTIT"
     gene            13903..14085
                     /locus_tag="ACGI8A_002448"
                     /pseudo
     CDS             13903..14085
                     /locus_tag="ACGI8A_002448"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            14113..14328
                     /locus_tag="ACGI8A_002449"
     CDS             14113..14328
                     /locus_tag="ACGI8A_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFH0180442.1"
                     /translation="MVPPKGKFFFGAVVVGERGQIVIPKEAREVFNINAGDKLLVLGD
                     EERGLGIVHQRDLINFIGHVGVVHKEE"
     gene            14360..15547
                     /locus_tag="ACGI8A_002450"
     CDS             14360..15547
                     /locus_tag="ACGI8A_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide family glycosyltransferase"
                     /protein_id="HFH0180443.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            15739..16143
                     /locus_tag="ACGI8A_002451"
     CDS             15739..16143
                     /locus_tag="ACGI8A_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HFH0180444.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         6398 a         1702 c         2867 g         5285 t
ORIGIN      
        1 ataacaataa tgaggatata aaaatgtatt tgctatatat ataattatat acatagatat
       61 ctaatattga tatttaatat gaaagtattg aaaatagaca atattaatta tagtgagtaa
      121 aaaatataat tggattttta aattgaatta tttaaaaggt atagtatgaa aaaaatctta
      181 taactaaata tgagatagaa attataaaat tttatactgt gaaagaggta gatgcatgaa
      241 attagggaaa gattttttat ggggaggggc aatttcagcc aatcaaagtg aaggatttta
      301 tttagatgga ggaaaaggtt tatctcagtt tgataaactc ccattaaatg ataagagatt
      361 aaaaaatgta tgtttagatg aggataatat tttaaatgaa aataatcaat actatccaag
      421 tcatataggg attaaatttt gtgaaaatta caaagaagat atagcaatgt tagcagaaat
      481 aggttttaaa tgttttagat tttctatatc ttggtcaaga atattcccaa caggagaaga
      541 aagtgaacca aatgaaaagg gattacaact ttatgataat ataataaaag aattaaaaaa
      601 atataatata gagccattaa taacgataag ccactttgat ataccttatt ctctagttga
      661 aaaatattct gggtgggctg atagaagagt ggttaattta tatgtaaaat atgctgaaat
      721 tttgatgaaa agatataaag atgatgtcaa atactggatt ccttttaacg aaatgaatat
      781 gataatgcat attccatata taggtgctgg gttaactttt atagctgatg aaaatcaact
      841 tcagaaaaaa tatcaagcag cacaccatca attattagct aatgcaaaaa ctatagaaat
      901 aggaaggtat ataaataaag aatttaagtt tgggtgtatg ttagctgctg gaaaaacata
      961 tccatacaca tgtaaaccag aagatgtatt tgcaacttta gagcatgaga ggcataattt
     1021 gtttttttct gatgttcaag ttaggggtgt gtatccaagc tttatgagtt attatttaaa
     1081 atcaaatcaa ataaaattaa taataaaaga ggaagattta tcaacactta aggataatac
     1141 tgtagatttt gtatctttta gctattattc tagtgcttgt agttcagcta gagctgaagg
     1201 tttagaaaaa tcaaaggcaa acggaccaga aaccataaaa aatccatatc ttccaaaatc
     1261 aaatagtgta tggcaaaacg accctgtagg gttaagaata actatgaatc agttatatga
     1321 aaggtatcag ttacctttat ttatagtaga aaatggactg ggtactcaag atattgtaaa
     1381 tgaagatgga aaaataattg atgattatcg tatagattac atgaaagaac atattaaaaa
     1441 tatgattctt ggagtaaatg aagatggaat agagcttata ggatatctta tgtggggatg
     1501 tatagatgta accagtgtaa gtgaaggaaa gatgacaaaa cgatatggca taattcatgt
     1561 tgattctgat gactatggaa gaggctcatt taaaagaact aaaaaacaaa gtgctttttg
     1621 gtataaaaaa gtaatagaaa cagatggaga aattttattt aatgatatgg aaactcatgc
     1681 ataattcatt ttaagaaagg agtgttatta tgagtttaat atttactact aaggaccaac
     1741 ttgtagagct agggtttagt aaggatacaa taaaatccaa tgaaacgttg tttacactag
     1801 ctaatggaca tttaggagtt agagggaata ttgaagagtc tgattttaat aatgagtatg
     1861 tagataatat gggtacatat gtaaatggat tttatgaaga atctccaatt acatatggag
     1921 aaagcgctta tggctatgca aaacaaaatc agactatctg taagttacct aatgctcata
     1981 tagtaaattt ctctattgaa ggagattgct ttgatttatc aaaaggtatt acaagtgaac
     2041 atactaggat actagattta aatgaaggga ttcttaaacg ttcatttata tgggaaaact
     2101 ctcgtggtag aaaagttaaa gtatctatag aaagattagt atcacaagat atagaagaat
     2161 taatgatgat ttcatataca ataacaccat tgaattttaa tggaaaaata aaatttaaaa
     2221 ataaaatgga aaatagtatg gagaagttta attgtgaaaa agtagatgac ccaagagtgg
     2281 catctataaa taagaagcaa tttaaaatta atgtaaaaaa atataacaat aagtatttta
     2341 tgaatttagt tacagaaaga agtagattag ggttatggtg tgggtttgat tcatatatta
     2401 cttgtggaaa tattacaaat gagacctata atgtttcaga tgaatgtatt gagcgagaaa
     2461 tagaagtcta tgccaaaaaa gatgaaaggt taaatttaga aattgttgta ggctatagta
     2521 gagtatattc tgatttaaat ctaaatgaag aacgtttaaa atcattagaa tgtctggagt
     2581 cgttaaaatg tttagaatca gaagcacatt taaaatcatt agaattggtt ttaagagaag
     2641 catgtaatat tggatttgat aaagtcaaaa atattcagag aaagaaaatg aatgagtttt
     2701 ggacaacctc tgatgtagag attgaaggcg ataaaactct tcaactgggg ataaaaacca
     2761 atttatttca tatttatcaa tcagcaggaa gagatggaaa gactaatata tcagcaaaag
     2821 gtcttacagg tgatggctat gaggggcatt atttctggga tacagaaatg tatatattac
     2881 cattttttat atatactcaa ccatccattg ctagagcttt attagattat agatactcaa
     2941 tcttagatgc atcaagagaa agagctagag aacttggaat aaataaaggt tgcttatatt
     3001 catggagaac tataaatggt aatgaatgca gtgcatatta tccagcagga acagcacagt
     3061 ttcatataaa tgcagatata gcttatggtg ttagaacgta ttttgaagca actaatgatg
     3121 aagaatttat gatgcaaaat ggcttagaaa ttcttataga aacatcaaga ttttgggttg
     3181 aatttggaga ttatatagaa tcaaaaaata ataaattctg tataaatgga gtaacaggac
     3241 cagatgaata tacagcaata gtaaacaata attactacac taatttaatg gctaagtata
     3301 atttaatata tgcagtacag atggtagata aatttaagca taatttattt tcaaatgaag
     3361 tatttagaaa agttaaatta caagaatatg aagtgaaaaa ctggataaat gccgctcaaa
     3421 atatgtattt accatatgat gaagagaaga aaataagcaa acaagacgat tcatttttag
     3481 aaaaatcagt atgggatttt aaaaatacac caaaagaaaa atatccatta cttctccatt
     3541 atcatccatt aacaatatat agatatcaag taaataaaca agcagatact gtactttcag
     3601 cgttgctttt ccctgatgag tttagcttag aacaaaagaa gagagatttt gaatactatg
     3661 aaaaaataac tactcatgat tcatctttgt caaggtcaat atttggaatg atggcaagtg
     3721 aaattggaaa tcatgaaaaa gcttataact attttatgga cacagcatta atggatatta
     3781 ataacctaca aaaaaataca agtgatggaa tacatgctgc taatatgggt ggaacatgga
     3841 tgagtttagt atatggattt gcaggaatga aaataaaaaa tgataaatta agttttgaac
     3901 caaggatacc taaacactgg aaaaatatta aatttaatat attatttaga gataatttag
     3961 tttttgtaaa tatagaagaa tataaatctt attataagtt aatcaaaggt agaaatataa
     4021 ccatattaca tgataataaa gaaataaaat tatcaaagaa cataatagaa gttaataatt
     4081 gttttaaagg gagtaattaa tatgaaagca gtcttatttg atcttgatgg agttataaca
     4141 gatactgcta agtatcattt tgaagcttgg catgagatag caaaatcgct agatatagaa
     4201 atagatgaaa cttttaatga aaaattaaaa ggcataaaca gggaagaatc attagataaa
     4261 atattgttat ctgctaataa agtagatagt tatactaaag aggaaaagtt aaaggtgtgt
     4321 gaagaaaaaa ataacattta tataaagtta ataaactcat taacatataa agatatatta
     4381 ccagggataa atgagcttat aaaagaactt aaaaaaaata atgtgaaatt gggaatagca
     4441 tctgctagta agaatgcacc taggatatta gaatctttgg gagtaaaaga tgaatttgat
     4501 tgtatagtaa atccatcttt attaaaaagg ggaaaaccat atcctgatat ttttattgaa
     4561 gggagcaaga tgctaaattt aaaacaatat gattgtgtag gaatagaaga ttcttattcg
     4621 ggtattaaag ctataaatgc tgccaatatg acatcaattg gtgtggggaa aaaagaagta
     4681 ctaggaaaat gtgaccatat tgtagaagat actagcttat taaattacaa tacactatta
     4741 caagcttgga gtaaaaagat taatttggat taaaattata ttaaaaatta ttttaaaaga
     4801 gaggataagg tatgaagaat aaatatgaag atgtagctca attgataatt gatggacttg
     4861 gaggaaaaga taatataatt gacttaactc attgtatgac acgattaagg ttcatcttaa
     4921 aagatgaaaa gaaagtcaat gcagataaat taaaatctat agataaggta gttggagtaa
     4981 actcaacttc aactcaatat caggtcataa taggtaatga ggtaggtgtt gtatttaatg
     5041 caataataaa taaggggata aataccacag gagatattaa aagcgatata aaaaataaaa
     5101 ataaaaaaaa tagtatttta aataatataa tagatacaat aactggttgt atgacaccta
     5161 tgataccagc ccttacagca tctgggatga taaaagttat attatctctt tgcaccactt
     5221 ttaattggct atcatcaaat tcaagtacat atagagtatt agatataata ggagatgtag
     5281 cattttactt tatgcctata atacttgcaa taaatgcagc taaaaaattt aatgttaata
     5341 catcaatagc agttatagta gtaggtgtgt ttttacatcc aaatttctct gcatgggtat
     5401 caagtgggga ccctatatca tttataggag taccaattca aggtgttata tatgcagcat
     5461 ctgttatccc tgcactttta acagtatgga tgatgtcata tatagaaaat ttcattgata
     5521 aattaacacc tagcatgtta aaaacaattt taaatccaac tttagtatta ttaataagtg
     5581 cgccaatagc attaatagta attggaccaa taggaaattt cttaggagag ggattagcta
     5641 gtataataaa tttactacaa ggaaggttag gatttataat ggtttgttta ttagcagctg
     5701 caatgccatt tatcgttatg acagggatgc atcatgcatt gactcctata ttcatatcag
     5761 catttgcaac tacaggacaa gaatcgctaa tattaatatc tcaagtttgt gctaatttag
     5821 cgcaaggtgg cgcaacatta gctgtagcat taagaagcaa agatagtaag atgaaacagt
     5881 tagcatcagc tgcatggata tcagctatta tgggtataac tgaaccagct ctttatggag
     5941 ttacattgaa acttaaaaaa cctgctatgt tagcatctat aggagcagga atcgcagggt
     6001 tatttgcagg aattactcat gtgactttat atgttccaca aaatagtata atggcagtac
     6061 ttggattatc aggggaaaag gggatttcaa atatagtaaa tggacttata gttatattta
     6121 tagcattggt tgctccattt attatgacat atgtttttgg agttgaaaaa aatgttaaat
     6181 aaagtacaga taatgtagta gaggaagtat aatattaact atttacatta atagaatttt
     6241 tattattatt gactataact tgttttgtat ttatattgat gcaaagaaat aagttaatat
     6301 taataagaat aattattata actgacaaaa agcaacataa aagattgatg aagtatatgg
     6361 aaataaaaag ttgacaaaat atgtctgatg ttttatcata ttaattggaa ttaggtagta
     6421 attgtttaag tacaagctaa gcttattcat taataatagc agtatcctag gaaggagtat
     6481 gcccattatt atggataagc tttttttatg cttaaaagga ggataatcat gataattaat
     6541 aagatattaa acaataatgt tgtaatcaca ttagatgata atgatgaaga agttatcgtt
     6601 atgggtaaag gaattggtta tcaaaaatca aaaggcaatc taatagataa aactaaagta
     6661 aataaagtat ttagaatatc aaataaagaa atatcaaaca aactacaaga attactcaat
     6721 aatattccta tagaacacat gaaattatca agtgaaatta tagaatacgc acaaataaag
     6781 ttaaacaaga agctaaatga aagtatttac atatccttaa gtgaccatac atattctgca
     6841 attcaaagaa tgaaagaagg aataaatgta aaaaatgcaa tcctttggga aattaaaaga
     6901 ttttataaag aagagtttga aattggaatg aaagcactag atattattga aaacaaaaca
     6961 ggtataaaat taccagaaga tgaggctgga tttatagcct ttcatatagt gaatgctcag
     7021 ttaagtgaag gtcatacttt ggcatctgat attacgaagt taatacaaga agtacttagc
     7081 atagttaggt atcattttag gatagaattt catgaagaat cagtgtttta ttatagattt
     7141 ataatgcatt taaagttttt tgcgcaaaga ttgcttttag atagtgcaca tgaaggtgag
     7201 acagacaaag aattactaag cattattaaa tctaaatata ataaagagtt tgaatgtgta
     7261 gttaaaatta aaaatttcat taaaaaacaa tataactaca tattaacaga tgatgagctt
     7321 atatatttaa caatacattt agctaaagta gttaaagact caaatatgta agtgaaataa
     7381 tttttcagca gtatattaag aatgattgaa aggagtgata ctaacaaaat tatacttatg
     7441 gttggatggt aacattgagt tggccaaacc ttcaaatttc aaaaaattaa tttaaacgaa
     7501 atatggagga gaaaaatggg aaaatacaaa caattagcac aagagatagt aaacaatgtt
     7561 ggtggaaaag aaaatattag tggattagta cactgtatta caagattacg ttttacttta
     7621 aaagatgaaa gtatagcaaa tgacaatgta ttaaaaaaca tggaaggtat tgttactgta
     7681 atgaaaagtg gaggacaata tcaagtagtt ataggaaatc atgttgaagc tgtctataaa
     7741 gatgtagttg aaataattgg tttagatgat tcaagtacaa gttcagaaac taaaaaatca
     7801 ggtaatatac tagataaagg tatagatata atatcaggaa tttttcagcc agtgttagga
     7861 ataatggcag cttgtggtat gttgaaagga tttaatgcat tatttgtagc aatgggatta
     7921 tatagtgatg tatctggtgg ttatatggta ataaatgcag cgggagatgc attatttaca
     7981 ttccttcctt tgttcttagg atatacttca gctaagaagt ttggattaaa accaatgttg
     8041 ggattagcac taggagctgc agtatgttat cctgctatac aaggtagcag tatatcagca
     8101 ggagcagatg tgatgtacac tttatttaaa ggaacaatgt ttgcatcacc agtatatatt
     8161 gatttctttg gactaccaat tgtatcaatt gattatactg gaacagtcgt accaattata
     8221 tttatagtgt attttgcttc taggtgtgaa aaattattta ataagtgtgt tccaaattta
     8281 gttaagtttt tctttgtgcc aatgcttaca ttattaataa cagtgcctgt tgcacttata
     8341 gttataggcc ctatagcaac atttggttct acacttattt cacaaattgt aatttcaata
     8401 agagatttta gtccactatt ggcaggtgct atagtaggat ttacttggca aattcttgtt
     8461 atttttggta tgcactgggg atttattcct gtatatataa acaatgttat gactttagga
     8521 tatgataatg ttatgatgcc attctttgcc tgtacatttg caagttcggc agttgtgttg
     8581 gcaatatttt tcaagactaa agataaaaaa ctaaaagaga tggcaatacc aaactttata
     8641 tctggtatat ttggagttac agagccagca atttatggaa tattattgcc tttaaagaaa
     8701 ccatttataa taagttgtat tgctagtggt attggaggcg ctttctatgg attctttaat
     8761 cttagaaaat ttataactgg aggaatggga atatttgaac ttccagcaat gattgaacca
     8821 gatgggggaa tgggtaactt aatagtagct ctttctggta tagctatatc aatggtcgta
     8881 gcttttgtat taactatgat tttatataaa gatgagaaag tagaagagtc taaaaaagta
     8941 agaaataagg gtaaagaaga aataaaagaa gtcaagtcta ctaaattaga aagagaaata
     9001 gtaacaagtc ctataaaagg tgaagtatta aaattaagtg atatagagga tgcagcattt
     9061 gcatctggtg tactaggtca aggggtggca ataataccta gtgatggaaa agtagttgct
     9121 ccagtagatg gggtagttac aaccttattt cctagtctac atgctatagg gattctttct
     9181 gatacaggtg ttgaagtgct tattcatatt ggattaaata ctattcaatt agagggaaga
     9241 ggttttgagg catgtataaa acaaggtgat agaattacaa aaggtcaaac tatattaaac
     9301 tttgatgttg atgcaataaa agagttgggg tatagtactg taactccaat tgttattaca
     9361 aattcatcac aatttttaga tgtagtagaa acagaaagta aaaatattga attagaagac
     9421 aatctaatta cagtcttatt ttagtaaaga ttgaggtatt agcataatct agtgctttgg
     9481 ttataatttt aggaggcttt gactatgagt tttaagaatg attttttatg gggaggagct
     9541 actgcggcca atcaatgtga aggtggatac aatgaagacg aaagaggatt ggcaaatgta
     9601 gatgtatgtc caacaggaaa agatagaaca gcagttatta cagggaaact aaaaatgttt
     9661 gattttgatg atgagcatta ttatccagct aaaactggta ttgatatgta tcataattat
     9721 aaagaagata taaagttatt tgctgaaatg ggatttaaag tatatagaat gagcattgca
     9781 tggagtcgaa tatttccaaa aggtgatgaa gaaacaccaa atgaaaaggg acttcaattt
     9841 tatgaaaata tatttaagga gtgtaaaaaa tataaaatag agcctttagt cactattaca
     9901 cactttgatt gtccaatgca tttagttaaa aagtatggtg catggcgtaa tcgaaaaatg
     9961 ataggtttct atgagaaatt atgcaatgtt atctttagaa gatataaagg attagtgaag
    10021 tactggctaa ctttcaatga aataaatatg attttacatg ctccatttat gggtgctggt
    10081 atttgctttg aagaaggtga agatgttgaa aagataaaat atcaagctgc ccatcatgag
    10141 cttgtagcta gtgcaattgc aactaaaata gcacatgaag ttgattcaaa taacttaatt
    10201 ggatgtatgt ttgcagctgg aagtgtatat ccttatagtt gtaatcctaa tgatgtatgg
    10261 gaagctacaa agttagatag agaaaattac ttctttgtgg atgtacaatc taaaggtaaa
    10321 tatcctaact atgcgttaaa gtatatggag caaaaaggta ttacaccaga aatggaacct
    10381 ggtgatattg aattattgaa taaatataca gtagatttta tttcattttc atattacaat
    10441 agccgttgtg ttagaaccga tgaaaatgca gatgatatgg ctgaaggaaa catatttaca
    10501 tctgctaaaa atccatatct ttcatacagt caatggggat ggccaattga cccattaggt
    10561 ttgagaatta cactaaatca tgtatatgat agatatgaaa agccactatt tgttgtagaa
    10621 aatggattag gtgctaaaga tatagctgat gaaaatggtt atgtagaaga tgattacaga
    10681 attgattata taagagaaca tataaaagct atgcatgatg cagtttctat agatggggtc
    10741 gatttattag gttatactac atgggcacct attgatttag taagtgctgg tacaggtgaa
    10801 atggaaaagc gttatggatt tatatatgta gatagagaca atagtggtaa tggaagtcta
    10861 agaagaatga aaaagaaaag ttttgaatgg tataagaaag taattgcttc aaatggtgaa
    10921 gatttatatt agacaagaaa tctatgttaa ataagaaatt tgtattaaat aagaagagta
    10981 taaaaattaa taataagtat aatttttaac ttttttaact atagaaaatc aatacagaat
    11041 tattttacat tgtatttatg caatagtatg attcacaaat aaaattttag aaaataaaat
    11101 tgttaagaag tatgggaagt aagtgcaatt tactttgtaa taaaagatga aaaaggcaag
    11161 cttaggtttt aagcttgtct tttttattat gtataaacta tgatacttta aaattctatg
    11221 aaaaatttat ttaatagaga aattaaaaat ttaatggtaa tcaaattctt taccaatgaa
    11281 tataaaaaat gtctacgtgt tataatagtt taaactaata aacgaaaggt agatgaatga
    11341 atatgaaata ccaacatata atagaagttg acaaagaact ttgcattgga tgtgggcttt
    11401 gtaaaaatga ttgtccagta aacaatataa ttatagaaaa taaaaagtca gtaattaaaa
    11461 aacaagattg tctaatgtgt ggacattgtg cagcaatatg tcccacaaaa gctattacct
    11521 tgacaggatt tgatgaacct cctatagaac ttacaaataa gccaaagcta gattcagatg
    11581 aattactaat ggcaattaaa tctagaagaa gtatacgtaa atttaaagac aaagaagttt
    11641 cttcagaaat tataaagcaa attattgagg caggtaggta tacccctagt gctaaaaatt
    11701 cacaagatgt atcatatatt gttttagaca ataaaaagtc aatttatgaa aacgaagcag
    11761 tgaaattttt tagaaaaata aaacctattg caaatatagc tattaaatat tcaaaagaag
    11821 ttgagattga cgataacttt ttctttaagc atgctcctat tgcaataatg ataattacaa
    11881 aagataagat tagtggttca cttgcagcat caaatatggc attaatggca gagtcttatg
    11941 gcctaggtgt tttatttagt ggatattttt cagatgtagc aaataattcg ccaaaattaa
    12001 aaaagctttt aggtctaaaa cgcagtaatc atgttcttac aacattagta attggatacc
    12061 cagatgttaa atatagacgt actgcacaga aagaagtagc aactgttaga tacttataag
    12121 agagggtgga ttttatggaa aaagaatgtc aacaaagaat gcaagaaata acgtctaaat
    12181 ttaaagaatg tcgcaaagct atctctgcta tgggtgatga gactagacaa ttgattttaa
    12241 ttgctctctt agagagtgat tttaatggta tacgagtggg agaaatcaca aaaaaaacac
    12301 atctttctcg tcctgctgta tctcatcatc ttaagatatt aaaagaagca gagattgtta
    12361 atttgcgtag agaaggaaca aaaaattatt attatcttga ttcaaaaaaa tcacaatgga
    12421 ggtcactaac agagctaata aatcttatat ttgttggaat acaacatatt agtgaaaatg
    12481 atataagaaa aggtgattaa attatacttg atttaataca aaaagattga tttaggaact
    12541 aaaaagaact aaaaagttca ctttgagaca ctatcttttg aactataccc atcttaggag
    12601 acagttaaaa tatgtaaaac tgtataaaac aatgggtata gttcatttta taatggttta
    12661 aatattaaga atatattatt ctaaatagta tctttctctg ttttgagttt tatttttata
    12721 ctcaatagct tcagtggggc aaccacaaat acatgccata caatgagtac agttattttt
    12781 ccaaactggt tttttgtttt ttaaattaat attatttaaa ggacatagct ctacacattt
    12841 tccacagcca atgcatttat cagtataatg aaatcctttg gcactaataa ttgtcttata
    12901 aaaaactgta tttattatac cacttttaat tgtacctcct aagccactag gagtaataat
    12961 ttcaaattca ttttcatttc gtatatcaga aataatccta tatatttgtt tatctgcttc
    13021 tttaatcatt tgttttgatg tttctttgtc tgttgtagaa aataaagcta tataattctc
    13081 tggcatttta atttctgcca taccttttaa ttgccattcc ttgtatttac atagtttttg
    13141 aatatagttt atggattttg ctgtatctcc accacaagtc attacaaagt aaactctgtt
    13201 gcttcctaga aattttgttt cttttataaa atccgtaacc actattggca atctccatgc
    13261 ataagtagga catacaaata tgaatggttt atctgttgat attaattcat ctgttttttc
    13321 ctcttttata agttgattta gtgatatcag ttcatcattt aattcttgtg caattttacg
    13381 agccacatat cggctattac cagttccgct aaaatataaa atcataataa tatctcccta
    13441 actatttttt attttaactc ttaatttttt atttattata gtaatttatt gatatattct
    13501 attcaaatgt atgtaaaatt tagtagttaa gaaaattata acacattagt aatttttata
    13561 cttattggta aagaatttga ttaccattaa atttagcaac aagacatcaa ctggacttgt
    13621 atatgagaag ggataataat ttgcttatga aagattataa atataaaggt tattttgaag
    13681 gtgtagaaat atttgataat gtaaatgacc ttaacttaaa tactttaaca acagttagta
    13741 gatttgtgtt tgtttttttg tttacgataa cttaaggaaa tatgttacct gaacaataaa
    13801 tttatataat aaaagtatat aaatatatat atgaatgtta agattagttg atagaattta
    13861 aataataaat ccaatattat aaacatagga ggtatgaaaa atatgattaa tgagaattta
    13921 aaaagtctaa gaaaaatcaa tcagtataca caagaagaat tagctgaaaa acttaatgtt
    13981 tctagacaat caatagcaaa atgggaaagt ggagaatcta ttcctgatat aggtagttgc
    14041 attaaacttg caaaattgta caatgttaaa ctagatgact tagttaatca ttcggaagaa
    14101 aaaacaggta ttatagtacc tcctaaagga aagtttttct ttggagcagt tgtagttggt
    14161 gaacgtggtc agattgtcat tccaaaagaa gcaagagaag tatttaatat aaatgcagga
    14221 gataaattat tggtacttgg agatgaggaa agaggattag gaattgttca tcaacgtgat
    14281 ttgataaatt ttataggtca tgtgggagta gtacataaag aagaatgatt taaaaaaatt
    14341 ataaaaggag gtatgaaata tgagtaaaat agtatttttt agcattcctg catatggtca
    14401 tactaatcct actattgaag tagttcgtga attagtggat agaggaaatg aggtattgta
    14461 ttattctttt aatgaattta aagataaaat tgaaggtgct ggagcaaaat ttatttgttg
    14521 tgataaatac cttccagaat tactccctgg agatgagaaa aaaattggta aggattttcc
    14581 tggattaatt gagatgatag tagatactac tatttcttta gatgaaaaag tttgtagaga
    14641 acttaaagat tttaatccag attgcattgt atcagattca ttaagttttt ggggaaagtt
    14701 atttgcacaa aaacttaata ttccatatgt atgctctaca acaacttttg catttaataa
    14761 acatacagca aagttgatga aacaaaactt tatagaaatt atacgtatga tttttggtgt
    14821 aagacgcatt aataaaaaaa taaagctttt acaaagaaaa gggtatgaag taaaaaattt
    14881 tatttctata gtttctaatg ataatgatac ggataccatt gtatatacat caaaagaatt
    14941 tcaaccaatg gtagaaacct tctctagtaa gtattcattt gtaggtccat cagtatcaaa
    15001 gttgattatt gaacctaaag agagaaaaag aaagctaata tatatttcgc ttggtacaat
    15061 taataataaa aatgttagct tttataaaaa ctgtataaat gcatttaaag attctgatgt
    15121 tgatgtgatt atgtctgttg gtagaagtac tgatattaaa agtttaggaa atataccaaa
    15181 taattttgag gttaaaaata gtgttgaaca gattgctata cttcaaaaaa ctgatgtgtt
    15241 tgttactcat tctgggatga atagtgttaa tgaaagttta tattatggag tacctatggt
    15301 attatttcct caacattcgg agcagagaat ggtagctaag agagttgttc atttgggagc
    15361 aggaattatg ttaaaagagg ataaatcaga aagtattaaa aaagcagtat ttcaagttat
    15421 aaatgacaat gaatataaag aaaatgcaac taaactatca aaaagctttt ataatgcagg
    15481 aggctcaaaa aaagcagcag atgtaatatt acagattata tgtaatagta gacaagttga
    15541 catataatag atttgttaaa aatatatata catacatgta atttaatcaa agtactgaca
    15601 gtggggtctt ttaacctacc agttggggca tatatattga tgctaaatat gagggatgtt
    15661 atattttaat aaaaaagata acatcctttt tttagtgcat tatatcgagt tttaaataat
    15721 aaatgaagga ggattaatat gaaaaaatat aaagttattt ttatagtatt aattataggt
    15781 attataagtg taacagggtt tgttgtatat aaaaactgca ttaaaccatg gaaagatgct
    15841 gaaacatata ttgataagta tatggtaaaa caagatattt caaaagataa tataaaaagt
    15901 attacaaaag aaaaagccaa gaaggcaagt tacgaaggaa tactatacaa agtgtattac
    15961 aaagatgacc ctcgatatga gtatcaatat ttttatagtg ataattatta tgctctatat
    16021 gtaaataaag taatgttaca aatttatgat atggaaaata ataataaact tttaagaagt
    16081 aatgaggaat taaaaagtgt aaagtatcca ccaatatatc ttaaatataa gccagataaa
    16141 tgatttatga caaaatagaa taatttttat taaaatttat ttaaaaatat cactagagcg
    16201 tttaaataaa actaaattta agtatttata tattagtata tcttactagt gt
//
LOCUS       DAYATN010000083        16044 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.083, whole genome shotgun sequence.
ACCESSION   DAYATN010000083 DAYATN010000000
VERSION     DAYATN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.083"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(70..1026)
                     /locus_tag="ACGI8A_002452"
     CDS             complement(70..1026)
                     /locus_tag="ACGI8A_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFH0180445.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            1394..2182
                     /locus_tag="ACGI8A_002453"
     CDS             1394..2182
                     /locus_tag="ACGI8A_002453"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFH0180446.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(2305..2775)
                     /locus_tag="ACGI8A_002454"
     CDS             complement(2305..2775)
                     /locus_tag="ACGI8A_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HFH0180447.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            2914..3558
                     /gene="uppS"
                     /locus_tag="ACGI8A_002455"
     CDS             2914..3558
                     /gene="uppS"
                     /locus_tag="ACGI8A_002455"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HFH0180448.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            3601..4506
                     /locus_tag="ACGI8A_002456"
     CDS             3601..4506
                     /locus_tag="ACGI8A_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0180449.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            4821..6368
                     /locus_tag="ACGI8A_002457"
     CDS             4821..6368
                     /locus_tag="ACGI8A_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFH0180450.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            6371..6769
                     /locus_tag="ACGI8A_002458"
     CDS             6371..6769
                     /locus_tag="ACGI8A_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFH0180451.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            6809..8542
                     /locus_tag="ACGI8A_002459"
     CDS             6809..8542
                     /locus_tag="ACGI8A_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFH0180452.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            8774..9985
                     /locus_tag="ACGI8A_002460"
     CDS             8774..9985
                     /locus_tag="ACGI8A_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFH0180453.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            10178..10438
                     /locus_tag="ACGI8A_002461"
     CDS             10178..10438
                     /locus_tag="ACGI8A_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFH0180454.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            10792..12504
                     /gene="ptsP"
                     /locus_tag="ACGI8A_002462"
     CDS             10792..12504
                     /gene="ptsP"
                     /locus_tag="ACGI8A_002462"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFH0180455.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(13296..13961)
                     /locus_tag="ACGI8A_002463"
     CDS             complement(13296..13961)
                     /locus_tag="ACGI8A_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180456.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            14305..15735
                     /locus_tag="ACGI8A_002464"
     CDS             14305..15735
                     /locus_tag="ACGI8A_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFH0180457.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT         6546 a         1562 c         2621 g         5315 t
ORIGIN      
        1 taaatacaaa gtaatttgca tttaagaggt atgtatttat catcaaatta ttggtttgtt
       61 ttggaatttc taatcttcaa ccataatatc tttaaatatg aaatcatgca ttgcatctag
      121 ttcatattcc tcaaaaggta ttacatagcc tgcattacct atttttctac cattttcagt
      181 agaaagagga aactccatag atttcaaatt taaatttcct atacttaaaa gttcttttcc
      241 taaagatatt atttcattag gattcatatt agtttttaca tacggaagta ttgtgttaac
      301 tagattagga tatttagtga ctggtaaatc cttaacacca tttattaaac tctgtataac
      361 acttctttgt cttctatctc tttcatctgt actatcattt tttcttatac gagaaaatgc
      421 aagtgtctga tatccattta atttttgctc accagaattt cttatatatt taagagaacc
      481 tttattgtca tttttattcc atttataagt ttcaggaata aatttattta attctcttat
      541 ttcactctta tcaacagtaa cagtaactcc acctaaagca tcaactatat ccataaaaga
      601 gaaaaagtca actacggcgt agttttgtat atcaagttgg aagttttctt ctatagtttc
      661 tattaataag ttagcttgtc catatacgta ggcatgtgtt aatttttgtt ctccacgtcc
      721 tggtatatta acataagtat ctctatttaa tgaagttagc ttaagacttt tatttttatt
      781 atcaatagta agtatcatca tggcatcaga acgagaaact tcttctcctg gtcttgcatc
      841 tgtacctaac aataatatgt ttgtgatatt ttcttcgttt ttatagtcat tactactaag
      901 cgcctctgta tttatactat catcttgtat agtttttaat ttagtaaaaa catatccata
      961 tatactaaca ggaaatgcta taactaaaag ggctagaaat ataacgaact ttttgagtcc
     1021 tgacaataat atcccctact ttctaaattt tttaattaca catagttaat tatattaaaa
     1081 atttgttaca taagcaataa aaaacacaat aaattagcat aagtttgtca taatataatt
     1141 atttttatag cattttatag aagaaagtgt gtagttatta ttgattttat tggaatataa
     1201 ttgtatgaag acttaaattt ttataaactg atgtgtatat tgaaaaaaat attttcctga
     1261 atgtagttat agcatcttat gaaaggtgca aattgtaatt taaaattttt agtgtataat
     1321 aagaattgaa aattcaaacc gataaaaatt aataaaaaat aattataaat taataatttg
     1381 ggggaggaaa tgtatgataa aattaatagc aacagattta gatggaactt tgttagatga
     1441 aaagagcgaa ataaatccag agttttataa agtatttaaa aaattaagag agagagggat
     1501 aatgttttct gctgctagtg gcaggcagta tcaaaatctt ataaaaaaat ttgaagatat
     1561 aaaagatgat atgatgttta tatcagaaaa tggaacacta gtagtatata aaggaaaaga
     1621 aatattatca aatcctttaa ataaagaatt agtaaatgaa atcatagaga caacaagaag
     1681 tataaaaggg aaaaaaattg taatgagtgg gaaaaaatac gcttatatag aaagcaaaga
     1741 tgaagcgttt atacaagagg tcagtactta ttatgcaaag tttaaaattg tagaagattt
     1801 gacgaaagta gaaggagaca tattgaagat agctgtattt gacttcaaag gtgcagaaca
     1861 taataataat atctactttg aaaagttttc tgatagagct caagtatgta tttctggagt
     1921 ggagtggctt gatttaactg caaaaggtgc aaataaaggg agtgccataa aaaaagtaca
     1981 aaaaatgtta gatataaaat atgaagaaac tatggtattt ggtgaccagt taaatgatgt
     2041 ggaaatgatg aaaagtgcct atcatagtta tgccatggaa aatgcaaatg aacatttgaa
     2101 acaaattgct agatttagag caaaaagaaa tactgaaaat ggcgttgtag ataagataaa
     2161 agaggttata aaaattggat agtcatatgt atagatgaaa aagattattt tgaagaaatc
     2221 aaaaaaatat caatgtaatt ttgataaata agtttattta tgcgaaaatt acattgatat
     2281 ttaataaaca tcaataatag atatttattt ttttatgata ttcagtattt tgttttctat
     2341 ttgagttgca ttttcagcag aaattaaata aacatatcta cctgccttct caattatagt
     2401 attatttgct atttcagaat tgattttttc tccatgtcct tctccaaaag gtgcttgtat
     2461 catatctttt ttataatttt ctagagtttt atagatttta tctacctcgt cagtttttat
     2521 aacgataata tcttctagag ataatttttc agaagaatta attacaaaac cttgaattat
     2581 gtcttcctct acatcattaa aatagttaaa atcctttgca tcaaagattt ttgattcctt
     2641 tataagtaaa tcagaagatt ttaatgcttt ttttattttt gatgtagaaa cgtttttgta
     2701 atcaccactt gaagaacaac taaccattcc taatgataag attatcgaaa aaaacaatat
     2761 taaaattttt ttcatgataa acctccaagt tttttaaact ctgtcttaaa ttagtttact
     2821 acataattat ataaaaataa atgtatttaa tgttacagtt ttgattaata atgtaatatt
     2881 ttgataataa tacctattga gaggtgatta gaaatgagaa taccaaaaca tgtaggaatt
     2941 ataccggatg gaaatagaag gtgggcaagt caaaatggta tgacaaaaga taaaggatat
     3001 gaaagtggaa tagaccctgg actagagtct tatagattat gtagagattt aggctgtgaa
     3061 gaggtgactt tttatggatt tacaactgat aatacaagac gtccatcagt gcagacaaaa
     3121 gcttttacaa aagcatgtat tgatgctgca aatatattaa tagatgaagg tgctgcttta
     3181 ttagttgtag gaaatacaaa atctccaatg tttccagaag aattattacc atatactaat
     3241 agaattaaag ccaaagaagg tcaaataaaa gcaaatcttc tggtaaatta tggttggtat
     3301 tgggacttaa ataatatatc aggagtagaa aatgtaaata aaaataatat acaggattac
     3361 atacagtcat ttgatgtatc cagactagac ttgataatca gatggggtgg aagaaggaga
     3421 ttaagtggtt tccttcctgt acaatcaatt tatgcagatt tttatgttat agataattat
     3481 tggccagatt ttaagagtga cgatataaag gacgcattta gttggtatga tacacaagat
     3541 gtcactttag gtggataatt aaaaatattc tgatttaagg ttgtagagtg ttttaaatta
     3601 ttgaaaaaaa aacttcttga tggtaaaata acactaataa tatgtaaaag tgtaaaaatt
     3661 tataccaaga agggtgaaga aatgtcaaaa ggcaataata acaataattc aagaaacaaa
     3721 tctaaaaaga ccagtcatct gaatagaaaa aagcgaaaat taaataagaa aaagttagca
     3781 gtgttgattt gctttacagt tttattttta tttattgcat ttaaggctac tcaaggtgta
     3841 gtagctttgg taaaaagtat ggataaaagt aataaaacta atcaacaaca aaatgttaac
     3901 tcagaacaat ttgaccttgg taatgaagag gaaaataaaa agaaaaaata tactgtattt
     3961 atagacccag gtcatggagg caatgataaa ggaactgaaa gcaagaccag taatagatat
     4021 gaaaaagacc taaatctaca aattgcaaaa aaattagcaa ataaattatc taaacaaaag
     4081 gatattcaag tagtagtatc cagaactgat gatacatata tatcattaaa ggatagagct
     4141 atcttagcaa ataatagttc tgctgatgtg cttgtttcta ttcatttaaa tgcagagaaa
     4201 aatggtaata cagcaacagg tatagaaact tggtatagga ataaagctac agatggttca
     4261 aaagaacttg cacagacagt acaaagtact atagtatcct atgttaaagt tagagatagg
     4321 ggaatagttg aaaataactt tgaagtactt agagaatcaa atatgcctgc tatattaata
     4381 gagtgtggtt ttttaacaac accttcagag gaacaaaaaa taataaatga aaaatatcaa
     4441 gaccaacttg cggaaggaat tgttcaaggt gtactttcat acctagattc taaagggaat
     4501 aagtaagtta actttacttt aatgtgagga taaatttaaa agtttaactg tgtaaattgt
     4561 attaacttta tttcgtaatg ttgcaaacat tgtttgaatt tagaaactat gaaattagaa
     4621 attattttat ctatatctaa tttcatagtt ttttttgcaa aaaatttata gttataaaat
     4681 tttaaaaaat aaatttagta taatgtaatg tagttattta tatgtgattt taacttgcat
     4741 tgatttgcta tcaattgtgc tttataatta gtatatataa agtttaactg taagataatt
     4801 taaaaaatag gggaatttat atgaataaaa atagcatgac aagaatttac agtttaaata
     4861 aaataatttt tattataatt ggaattttag gaatagtttt actcacaaga catattgagg
     4921 tagatattaa agttttaatt ttaattttat taaccttgtt tagtataaat gtatttattt
     4981 catatcttaa aattaagttg tatgaggata aaattaattt aggattgata aataattcag
     5041 ataagtttat aaatattact aaagatgaga acttcctaaa acatgttcaa aattatatta
     5101 tcagtaatga aaaagaaaac tgtgtaatgg cttgttttga tttatgtaga ctcaaactta
     5161 taaatgatat atatggttat gagctagggg atgaagtgtt aaataatatt ttaagtaact
     5221 taaaaaatta ttttggaaaa gaagctattt atggaaagtt aaaaagtgat gtttttgtat
     5281 taataataaa gcttgaaaat agggaacaga agattcctta tttagtaaat cttattaaga
     5341 ataaaattgt gatactaagt cagattgaca gttttaaaat ggatataaat attgaggctt
     5401 ctattggaat ttataaattt aacaataatg aaattgatat aaaaaaagct atagataatg
     5461 ctgatatggc aagacttaaa tcaaaaggac taaagcacat agagtatgta gaatttgata
     5521 atgctatgga agaagaaata caatcaatta tgaaaataga gagagattta ttcttggcta
     5581 ttaaaaataa agagtttgta attcactatc aacctaaagt tgattcaagt acaggtaaca
     5641 ttataggttc agaggcattg ataagatggt tgcacccatc attaggcatg gttggaccaa
     5701 ataagtttat acctatagct gaaaaaaatg gtctgataaa caatattggt agatggctaa
     5761 ttcaagaagt ttttattacc attaacaagt ggatttcaga aggtataaat gtcttaccag
     5821 tctcaataaa tctttctaga gtagaactat ataaaaatga tttagttgat ttttttaaac
     5881 tgatgtttaa tacatataat attccaaaag aacttataga attagaaatt acagaaacga
     5941 cagcactaag agatgtaaaa tttataagtg aaaggcttta tgagattaaa tctttaggta
     6001 tgaatatatc attagatgat tttggagtag gtaattcaaa ttttatcaac cttaagggga
     6061 taccactaga tattataaaa attgataggt cacttatatt ggatatagtt acaaatacaa
     6121 aaacaaagtt tatggtgaaa gcaattgtta acctttcaca tgatttaaac gtaactgtta
     6181 tatgtgaagg ggtagaggat atgcatcagg tcaaagtctt aagtgaactg ggatgtaatt
     6241 tcatacaagg ttatgtattt tttaaacctt tggatgagat gaattataaa aaacttctta
     6301 ctgacggaag ttatataaat ctgaaagata ctcttttaga taaatgcatg acagttgagg
     6361 aggagtaagg atgatttcag aagatgtaga ttattcaata agaatcataa gagctatatt
     6421 taataatgga gaagataaac caatagatgc taaaatgata ttaaaaagtg aagatatacc
     6481 aatactaaca ctatacagac tgcttagaaa gctttgtgca aacaatattc ttacttttaa
     6541 caaagaagat ggcaagacat acatgttaaa tgtgtcattg gatacaataa gtctaagaac
     6601 tattgtagaa gtaattgatg gaaaaatata tataaataaa tgtacgagat atgtatctaa
     6661 ttgcagtaat tgcttaaaag gttgtaaagt aaatcgtgaa ttgctagaaa tagaaaaaga
     6721 aatttataaa cttttatcga gaaaatctat aagacaaata ctagaataaa aaattttcag
     6781 ctatgataat aattggaggt tttcggttat ggatgatata tctcaagata attttctact
     6841 gtctaaagag tatgagaata gcttagatgt agatactaaa aaagcaagtg gaatatatta
     6901 tactcctaaa ataattgtag attatattgt aaaaaaaact ttaaaaaatc acgatataat
     6961 taaaaatcca tatccacgaa tacttgatat ttcctgtggg tgtggaaatt ttcttttaga
     7021 agtatatgat attttatatg acttatttga ggaaaatata tatgaattga aaaagaagta
     7081 tgatgaaaat tattggactg ttgataacat acaccgtcat atattaaact attgtatata
     7141 tggtgctgat attgatgaaa aagctataag tatattaaaa gatagcttaa caaataaaaa
     7201 ggtagtaaat gatttagatg aatctgatat aaagattaat ctattttgtt gtgatagtct
     7261 taaaaagaaa tggcgatata agtttgatta catagtgggt aatccaccct atattggtca
     7321 taaaaaactt gaaaagaaat ataaaaaatt tctcctagaa aaatatagtg aagtttataa
     7381 ggataaagct gacttatatt tttgttttta taaaaagatt atagatattt taaagcaagg
     7441 agggattggc agtgttataa ctccaagata ttttttagaa agtctatcag gtaaagattt
     7501 aagagaatat attaaatcaa atgtcaatgt tcaagaaata gtggattttt taggtgcaaa
     7561 tatatttaaa aatatagggg tatctagttg tattctaact tttgataaga aaaaaacaaa
     7621 agagacatat atagatgtat ttaaaatcaa aaatgaggat atatgtataa ataaatttga
     7681 aacattggaa gaacttctaa agagtagcaa atttgaacat tttaatataa atcagagatt
     7741 gctatctgac gaatggatac ttgtaaataa agatgatgaa actttttata ataagataca
     7801 agaaaaatgt aaatatagct tggaagatat agcaataagt tttcaaggaa taattacagg
     7861 gtgtgataaa gcgtttattt taagtaaaga tgatgtaaag ttgaaccttg tagatgataa
     7921 atttttaaaa tgttggataa aaagtaagaa tataaataaa tatatagtag ataaaagtga
     7981 atataggcta atatactcaa atgatataga taatgagaat acaaataaaa ggatactaga
     8041 tgaaattata gggctatata aaacaaaatt agaaaacaga agagaatgta aaagtggaat
     8101 aaggaaatgg tatgaacttc aatggggtag agaaaagctt ttctttgaaa gaaaaaagat
     8161 aatgtatcct tataaatcaa atgaaaatag atttgctata gattatgata ataatttttc
     8221 aagtgcagat gtatattctt tttttataaa agaagagtat ttagataaat tttcatatga
     8281 atatctagtt ggaattttaa attccagtgt ttatgataaa tactttaaaa taactgctaa
     8341 aaaaatgagt aaaaatatat atgattacta tcctaataag gttatgaaga taaggatttt
     8401 tagagataat aattatgaag agatagagaa tttatcaaag caaataatat caattttatt
     8461 aaataagtct atagataaag gaaaagtaga aaaattgcag ataaaaatgg ataacttgat
     8521 aatggattct ttaggtatat aagcagttgt aagtattaaa aaattataat cttttaattg
     8581 tttatttaaa tttagaaata aaaagttagc catataaaaa atattaaaaa tatatacttt
     8641 tcaatatttt tttgataaaa taagaatgaa tttgtgcaat tttatttttc tccaataact
     8701 aaaaatagtt ttttgaatta atatttgctt aagaataaaa atcagaaacg tttttgatat
     8761 agtaggtgat aatatgaaag gattagtttt agaaggtgga ggaacaaaag gagcctacca
     8821 aataggtgca tataaagctt taagagagct tggtatagag tttcaaggag taactggcac
     8881 atcaattggt gctttaaatg gagcatatat agttcaaaat gatattgaag taatggaaga
     8941 tatttggtta aattatgact atactcattt tatgaatatt gatagagaaa cctatgagag
     9001 gtttaaaaat gtagatttca ctactaaaaa tctcaatgca gtgtttgagc ttataaataa
     9061 agctagaaaa aatgaaggta tagatataag cccattaaga caactaatgg agacaacatt
     9121 aaatgaagac ataataagga aatcaaaaaa agactttgga cttgttacgg tctggtggga
     9181 taaaaaaatt aatccatatc cattgtatat cgaagatata cctaaaggaa gattgattga
     9241 ttatttaatt gctagttcca gtttgcctat ttttcaaacg aacatgatgg atgataagtt
     9301 gtatcttgat ggaatgtttt gtgataacat gccaatagga ctcttggaag acaaaggtta
     9361 tgaagatgtt gttgtaatta ggttgattga tgattttatt gggaaaatga atctaaatcg
     9421 acatcaaaat ataaatataa aaactataat accatcagaa tatctaggag gttctcttaa
     9481 taaagataga gataatgtag aaaaaaacat ccgactaggt tacttagata ctatgaaagc
     9541 atatgaaaga tgcactggaa ttaagtatta ttttgatgta gagtataaat ataatgaaga
     9601 atattgtttt gataaattta gaagtatgag caatgaaaca atacaatata tctgtaattt
     9661 attgaatata aaaagagatg cttcacttag aaccgttatg gaaagtgtaa taccaaagct
     9721 tggtgaggtg cttggattgc caaaggattt ttcatataaa gatttgtttt actgtatata
     9781 tgaaaagaaa cttgaagaaa ataatataaa caggataaaa ttatatgatt ttaacaaggt
     9841 tatagatgtt gtaaatacaa atattgattc taatagaaat ttagcttcta attttgaagt
     9901 tagacctaaa attagtatta aaaagaacaa aaacacaaaa ttattgacaa attgtattat
     9961 aaaagacata aaaaatcaaa gttaaataaa aaacaaaaaa aaatttgtaa ttatactcga
    10021 aaaaagtgta tattagtagt ataatatttt tgtagcacta gtgggaacaa aaaatgacca
    10081 taggacaaaa aagtaccact tagtaccatt aaagtaaaaa acaggaaaac aattttaaaa
    10141 aatataaaat gtaaagttta taggaggatt taaaaaaatg gaaaaagtag ttagtatcaa
    10201 aaatgcaagt ggattacatg ctagaccagc aggaatgttt gttaagaagg cagctgaatt
    10261 taaatcaaca gtagaggttg tagcaaaaga caaaactgta aatgcaaaat caataatggg
    10321 aataatgagt ttaggtttag ctcaaggaga agaattaaag ttagttgtaa atggagaaga
    10381 tgaagaagta gctttagctg caatggttga attagttgaa agtggatttg gagaatagat
    10441 ataataattt tttaaaaatt tgatatttag aatctaatat tacttaaagt aaagtaacag
    10501 agaagtatta aacatataca gaccaacatt tgtagatatt taatttcttt gttactttca
    10561 tgtttattta atcagaagat taagactatt gttaattggg tataaaaata taaatatttt
    10621 agaaaatgaa ataagatatt tttaatcata aattcagagt aaaaatacag taacaaatag
    10681 tttataaaat tttataaaaa attataagta aataaaacta taaaaatgta aattatttat
    10741 ataaatttat aaagtttatg tgctagatga tattttttag gaggtagggt tatggcttac
    10801 aaaggaatag gtgcttctcc tggtgttgct ttagggaaag cgttagtagt tgaacatagt
    10861 gaattagtaa ttgaaaaaaa atctatagat aatgttgaag ctgaaattgc taaattagaa
    10921 gatgcagttg cagtttctaa agaagaatta gtaaaagtta aggaaaaggc ttctgaagaa
    10981 ttaggagcag aggaagcaga aatatttgaa gcacatttat tagttttaga agacccagag
    11041 ttaattggtt ctgcaataga taaaattaaa actgaaagtg taaatgcaga gtatgcttta
    11101 aatgaaataa aagagatgtt tgttagcatg tttgagtcta tggacaatga gtacatgaaa
    11161 gaaagagcgg cagatataaa agatgttaca aatagaatac ttagacatat attaggaatt
    11221 aaagttgttg atttatctgc tttaagcgaa gaagttgtat taatagcaca tgatttaact
    11281 ccatcagaca cagcaactat gaataaaaaa atggttttag gatttttaac tgatataggt
    11341 ggaagaactt ctcatactgc tatcatgtct agaactttag aaatagctgc aatcgttgga
    11401 ttaaatgata taacttcaaa agttaaggat ggagattttg tggttttcaa tggagacaca
    11461 ggtgaagtaa tcgtgaatcc tgatgaagag actataaata aatatactga attaaaagct
    11521 aagtatgaag atgagagaaa agcattacag ctattaaaag gaaaaccatc tgtaacttta
    11581 gacggaaaac atgtggaatt agctggtaac ataggaacac ctaacgatat agaaggtcta
    11641 ataaagaatg atgctgaagg tgttggtctt tatagaacag aatttttata catggataga
    11701 gattctttcc caacagaaga aatacaatat gaagcatata aagctgtatt agaaggaatg
    11761 gatggaaagc ctatagttat aagaacattg gatataggtg gagataaaga attaagttat
    11821 ttaagtatgg aacctgaaat gaatccattc ttaggttaca gagcaataag actttgccta
    11881 gatagaaaag atatatttaa gactcaattg agagctttat atagagctag tgttcatgga
    11941 agattaagaa tcatgttccc aatgatttca tctttagaag agttgttaca agctaaagaa
    12001 gttgtaaaag aagttttagc agaacttgat tctgaaggtg tggcttatgc taaagatgta
    12061 gaaattggta tgatgataga agttccatct gctgctgtga tatctgatgt attggctaaa
    12121 cacgtagatt tcttctctat aggaactaat gatttaattc aatacacttg tgcagttgat
    12181 agaatgaatc aaaagataag ctatctttac aatcaattta atccagctgt tttaagactt
    12241 ataaagactg ttatagacaa tgcacataaa gaaggcaaat gggcaggtat gtgtggagaa
    12301 tctgctggag accaaaaaat gataccaata cttttaggta tgggattaga cgagttctct
    12361 atgtctccaa tatcaatact tcctgctaga aagttaataa cttcagttaa agagtcagat
    12421 atgaaaaaat tagctgatga tgtacttaat atgggtactg ctgaagaaat aaaaagctat
    12481 atagaaaaga cttttaatat ataattaatg ttatatagtt actaaaattc atttttctaa
    12541 ataacaaaat tatattttac gaattaaaaa ggagatgttt caaaataaaa atttgaagca
    12601 tctctttttt ataaaagatt actaagaatt acttaatctt tgtaatagtc attttgtgaa
    12661 aaagcaaaac tgcattgtgg ctatagtata tatggatgtt attatcaata aaagtactat
    12721 taaaatgaag attcattctc aaatgatatc aaaagtagtt gtgatggagt aagataatat
    12781 tttgtgattt aatagtaaaa attaaaaaaa tatggtattg aaaaataatt aaaagtattt
    12841 aataatagaa ttagggaaga ataagtttat aagtaaaaat aattgattac tagttaataa
    12901 aataaatgtg atatccaaat aaatcctaaa tctaatttga gttttaatat agatagtcta
    12961 aagtttgatt attaaagatt taattgtaag ttgttacagt tgatgatatt gattagagat
    13021 taaagtttag agtagaaagt taccttaaat ttaaaaatgc aagataaccc aatctaaaaa
    13081 ttataatcac tcaataggtt taaggattta tttgtttatt ataaatagga gggtgtctca
    13141 aaatgaactt tttagtttat gaggcactct cttttatata gaattaaata tattttatta
    13201 tttcaacaat gaaaaagaag cttatctcta ttttgagaca gcctctttgt atactattat
    13261 taatttaatc aataacataa tttaaaacta tattattagt taagatttcc tgctttatta
    13321 gtaaataatt ctttaatagc attgtttctg gaaactaaat cgttatcaga gttgtttgtt
    13381 tttttcataa tcactgaata acgtttttta tttataaaaa atattaaaat cttatcatca
    13441 tcaaaattgt attctttata tctgtcaata gactcataac ctagatttag aatgtttttg
    13501 tcttttatac tatctacatc taaaatatat gatgggtcag cctttagtaa tatattaatt
    13561 aaacattttt cttcaccagc acagaataac tccatcttac ttgatataga tttagattgc
    13621 tctgaagtta tttttgcagg aataaggttg tcattgtcat ctcttagaag ataaccatgt
    13681 gtgtcttgtt gatatactaa attaaccttt ttcttaggaa cactaatcat tttatctaaa
    13741 tgtacataat gatgattttc aacaaaacct tcatttaata tttcaacaaa gttattaatt
    13801 ttacctaggc aaaaagattc tatagattct ttacagaatt tatttgtaaa tggtattatg
    13861 tttacgtttt cctcgtctgt attccatgct atagcataat ccaacgaata tacttcttcc
    13921 tctatatctg caaaattata ttgtactatg tttcctttca aaattactcg acctccatct
    13981 tataatatat ttaatatact ttataaaact tatattatat ttaaataata tcataaattg
    14041 aataaatatg atatagtaaa taagaaaaaa tttgaaaaaa atcactataa atgagtaata
    14101 aatataaata gtgtatacta atgggatgga aaatgtccac tattaacttt attaccccac
    14161 atatataaga taaaattatt aatataaaat ataatctatc gacaatgaat agacttttaa
    14221 ataaacttaa tataaaatta attaaataag tgcttaaaaa cgatttactt taatgataga
    14281 atcatattaa aggaggataa ttttatggat ataaagaata aaattaaatc tatattttta
    14341 ccagaaaaaa atgactattt agatgatgaa gaatttgagt atgatataga aaataatgaa
    14401 gaagatttaa aggaagataa caaagtacat ttaaatgatt taagcagagt aaaatatgat
    14461 tacagagagt taaaagacat agaaaaccaa acagaagaga tgtgtctgct agcagtaaag
    14521 caggatggta ctataattga gtttgtaaag gacaagactt ataaagtatg tatggaagca
    14581 gtaaaacaga catataaatc tcttaagtat ataacaaatc aaaatgagga tatttgtata
    14641 gaagcagtaa aacagaacta cagagctttg tactacataa ataataagac agaaaatgta
    14701 ctaattgagg ctataaaaaa tgcatcaact tatgatgtca tggaagtatt taaatttgtg
    14761 gaagagcaaa cagaagatgt atgtctagca tttatagaaa gggcatctaa aaatgatgtt
    14821 gcagagatat taaaaggaat aaaagagcaa actccagcca tatgtttaga agctgtaaaa
    14881 aaagatggaa aatctttagc ttatgtaaag gaacaaagta atagtatatg tttagaagct
    14941 gtaaaagaaa actacagtgc actatcttgt gtaaaagagc aaactgaaga aatatgtata
    15001 gaagcagtaa aacagaatga ctttgcattg tattatgtaa atgaacagac agaaaaaata
    15061 tgcatggaag cagtaaaaag aagttatatg gctctacagt atgtaaataa gcaaacagaa
    15121 gagatttgtt tggaagcagt aagaattgat ggaagagcat tacagtacgt aaaagagcaa
    15181 acagaagaaa tttgcttgga gtcagttaga caaaatggaa aagtactgca atatgtaaaa
    15241 aaacagactg aaaatatatg tatagaagca gtaagaggtt catttgaaga acttgaaata
    15301 aaagaaatat taagttatgt aaagatacca acagaaagaa tatttgtaga agcagtaaag
    15361 caaaatggaa aaattcttaa atatgtagaa aatcagacag aattaatatg tttagaggca
    15421 gtaagagaaa actataatgc tttagcttat gtaaaggaac aaacagaaaa gatttgttta
    15481 gaagcagtta atcaaagtta tgaagcgctt aaatatgtga aagaacaaac agaagaagtt
    15541 tgtctaaaag cagtaaaaca agattataga atgttgaaat atgtaaataa tcaaacagaa
    15601 aaaatatgtt tagaagcagt aaagcaaaat tatagagctt tagaatttgt tgataatcaa
    15661 acagaaaaag tttgtcttga agcagtaaaa cagaatagaa aagcactaca gtatgtaaaa
    15721 caaaaacaaa gttaaaaggc taaaatatat ggggaatata tgcaaataaa taaaatttga
    15781 tattttgtaa taaataaaat gggtatctca aaaggaactt tttagtttgt gaggtactct
    15841 ttttatatga aatgaaatat atttttagcc ttttaacaaa ggggaaaagg tttgtttcta
    15901 ttttgacata ggctttttat gcatataaag cttgataaaa ttgttttatt ataattgttt
    15961 tgttatataa ataataagaa gaaaattata taggtctttt tttgaccagt tttacatttt
    16021 taacaactag ttttgtaaag tata
//
LOCUS       DAYATN010000084        16010 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.084, whole genome shotgun sequence.
ACCESSION   DAYATN010000084 DAYATN010000000
VERSION     DAYATN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16010
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.084"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(198..683)
                     /locus_tag="ACGI8A_002465"
     CDS             complement(198..683)
                     /locus_tag="ACGI8A_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFH0180458.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(766..859)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1414..1596)
                     /locus_tag="ACGI8A_002466"
     CDS             complement(1414..1596)
                     /locus_tag="ACGI8A_002466"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0180459.1"
                     /translation="MLNKKLPDTELKIMKCIWHTGYKTVISKDVADEIEKTYGWKHTT
                     TIILLARLTKKGFLIS"
     regulatory      1822..1919
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1974..2150
                     /locus_tag="ACGI8A_002467"
     CDS             1974..2150
                     /locus_tag="ACGI8A_002467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180460.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            2376..2576
                     /locus_tag="ACGI8A_002468"
     CDS             2376..2576
                     /locus_tag="ACGI8A_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFH0180461.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            3328..3510
                     /locus_tag="ACGI8A_002469"
     CDS             3328..3510
                     /locus_tag="ACGI8A_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFH0180462.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            3581..4426
                     /locus_tag="ACGI8A_002470"
     CDS             3581..4426
                     /locus_tag="ACGI8A_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0180463.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(4650..4814)
                     /locus_tag="ACGI8A_002471"
     CDS             complement(4650..4814)
                     /locus_tag="ACGI8A_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180464.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(5356..5688)
                     /locus_tag="ACGI8A_002472"
     CDS             complement(5356..5688)
                     /locus_tag="ACGI8A_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFH0180465.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(5932..6573)
                     /locus_tag="ACGI8A_002473"
     CDS             complement(5932..6573)
                     /locus_tag="ACGI8A_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFH0180466.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(6554..6847)
                     /locus_tag="ACGI8A_002474"
     CDS             complement(6554..6847)
                     /locus_tag="ACGI8A_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HFH0180467.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            7228..7299
                     /locus_tag="ACGI8A_002475"
                     /pseudo
     CDS             7228..7299
                     /locus_tag="ACGI8A_002475"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7470..7604)
                     /locus_tag="ACGI8A_002476"
     CDS             complement(7470..7604)
                     /locus_tag="ACGI8A_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180468.1"
                     /translation="MNISRRAMKIIELAQKIANKRGVTVQDAWNDAMKEYKEKYEYVA
                     "
     gene            complement(7815..8567)
                     /locus_tag="ACGI8A_002477"
     CDS             complement(7815..8567)
                     /locus_tag="ACGI8A_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFH0180469.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(8626..10281)
                     /locus_tag="ACGI8A_002478"
     CDS             complement(8626..10281)
                     /locus_tag="ACGI8A_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0180470.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     YQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(11554..12633)
                     /locus_tag="ACGI8A_002479"
     CDS             complement(11554..12633)
                     /locus_tag="ACGI8A_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0180471.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIFNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(12717..13373)
                     /locus_tag="ACGI8A_002480"
     CDS             complement(12717..13373)
                     /locus_tag="ACGI8A_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0180472.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            13734..13901
                     /locus_tag="ACGI8A_002481"
     CDS             13734..13901
                     /locus_tag="ACGI8A_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180473.1"
                     /translation="MPFIIFPTIEKKSIEEWVNIGANMLCLAQDTTFITYVINQLLSF
                     YNEIVPIQKQH"
     gene            complement(14054..14482)
                     /locus_tag="ACGI8A_002482"
     CDS             complement(14054..14482)
                     /locus_tag="ACGI8A_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFH0180474.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(14958..15896)
                     /locus_tag="ACGI8A_002483"
     CDS             complement(14958..15896)
                     /locus_tag="ACGI8A_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HFH0180475.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         5551 a         2575 c         1685 g         6199 t
ORIGIN      
        1 tttatgatac ttggtgtata aagtataagt taaatctact ttcaattaaa tttaccattt
       61 ataattactt tataataaaa ataaattagt taatttattc tctttagttc tgttataatg
      121 tttctacaaa aaagaatccc attaatggga ttcttttttg cgatttatta atatatttaa
      181 agcttgctag tatcgtatta taaacttaaa tgctcttcta tgtattgatt atatacatat
      241 ccagaatatc gtttttcatt tttattgata ttaactcttg cccattttcc aacgctataa
      301 catacttcaa ctttaaaacc atcagggatt gtagttataa ctttagattc taatgttcta
      361 tcatttctaa gttctaaact taatccattt gttcttatat ttcctatgta attctttttt
      421 ctatttttta ttgtatcatt actattgttt ttagcattac ttaatctttc taaatattta
      481 taaagtaacc aacctttgtc ataattactc tcaacttctg cccatattcc atctacacac
      541 tttaacttaa caattttacc ttctggtata ctttttaatt tcaaactatt tatgtctggt
      601 ttttctctta aattcaattt aattttagtc ttaaaattga tgctagattt gcgaaaattt
      661 tttaacatta attcagatat catcttaagc cctcctcttg ccaaatatta agttattaaa
      721 atatataact ctaaaaatac ttttaacaaa aagaattatt atctttttgg caactggctg
      781 acatgacctt tattacataa agattttagt ccctatagtt ttgcgtcatt agatttatct
      841 aatattgcct atatctgaat aataacataa tttgaacaat taaaaaataa tattacctca
      901 aattttatat tatttaaata taaattaaat ttttaattaa taataatcaa aaaatatctt
      961 tatatttatt caattactgt tctttatagt caatataaaa gacagtaatt tatctatagt
     1021 gttacttata ttttatatat atggctattt ttcaacattt tacaatatat aacatttatt
     1081 tttttaactt ttgtcagtaa aatgtcagca aaacacttta tccacaattt ttatataagt
     1141 tttccacaac ttatcaccaa tattatccac ataaaaaagg tagcacttat attattagct
     1201 accttttatt tattaatctt tatatttcca gctttttatc caacttccta tttctattat
     1261 tttttcttca gttatttctt ctccatcatg taattttgaa attaattctg ataaaggatt
     1321 gttatgtaaa cctctaaaaa ctctttttcc ctctattttg agatattctc tctcttttat
     1381 taatactgta tagtgtacat gcctcccaat tctctatgat attaaaaaac cttttttggt
     1441 taatctagct aaaagtataa ttgtagttgt atgcttccaa ccatatgttt tttctatttc
     1501 atctgcaaca tcttttgata ttacagtttt ataaccagta tgccatatgc atttcataat
     1561 ttttaattct gtatctggta attttttatt tagcataata tattccttct ttcaatttat
     1621 gtcacttctt caaatttatc tagcttaaat tataatagga gctttttaga acaaaaacta
     1681 tatttaattt ttaccaggta ctctacgtgt ttagtctgtt tttaagtgta aaataatgtt
     1741 catatctata aatttattta actttcaaac aatcctgttg aataataact aattctattg
     1801 tattatttta gttgtagaat aaaactaaat cggcaaaact agagaaattt agtgacgcaa
     1861 agctataggg actaagactt atataaatat cttatgagtt atgtcagcca gttgccaaag
     1921 agatagtgtt ctttttgttt ttatgaaagt tttttagggg gataataatt tatatgtttg
     1981 gaaataaaat ggataaatgt acacatgtat tgactgctta tattagcagt tcgtatgatt
     2041 attgtaattt tttagataca cagttagatg attttatatt agagtacgga gaaaatgtag
     2101 tagagtcttg tttacaccaa gtaatggtat tggtaagtaa gtataattag atagacattg
     2161 atttctagaa aacccttttt agttttatct acatttatat taatatatag caatgtaaaa
     2221 cgtttaaata tatttttaat attaagaata attaaaatgt taactaaaaa atataattaa
     2281 gtagacattt ctattaatct atgatacaat agtatttgtg gtaatacaca attaaatcaa
     2341 gtatatcttg atataggtta ataggagtat ttttaatgaa taatggaata gtgaaatggt
     2401 ttaataatga aaaaggattc ggttttatat caatggaagg tagagatgat gtgtttgctc
     2461 atttctcagc tatacaaact tcaggattta aatcattaga agaaggtcaa caagtaagct
     2521 ttgatatagt taaaggtgct agaggtcctc aagcagaaaa tataactata ttataatata
     2581 tagatttttc gggaaagact ctttttagag tcttttttta ttgttaatat atgtaagata
     2641 atcattggtg aagtaaataa ggtatattga gtaatgttct tgatagttta tatgctaatg
     2701 aatttcaaaa aaataagcac tcctataaaa agagtgctct taatgtaaat ataaaccttc
     2761 attggtccag cctaataccc cgtattctcc aagttctaac tattttttat atacattatc
     2821 actttcatcc atatctaaca caattatttc ttctttagaa tcaatttgta aaatatgagg
     2881 actgtgtgtt gtgacaatta tttgtgtttt taggagaatt gcttttaatg taataaaaat
     2941 tttacatacc acatagttga tataaaacta taataattac ataagattgt aataataaga
     3001 cttatcttta cataccacat agttgatata aaacaatgaa attaacatac aagagactaa
     3061 gacaattaaa atctttacat accacatagt tgatataaaa ccccaaaata aacttagcat
     3121 ctccaatgtt tacacatata caatgctctt aaaattgcag tgaacgcgca atagtgcaat
     3181 tgataatact tattatatat tctcaatgcc ttatatttca actgctaaac ctcaatttgt
     3241 cacaaatatc gctcactgca aaatttctat atttttatta tatcataaca tataattaat
     3301 attttcaatt aatttttaca tttaattatg tttaaattct ataataatgc aactttaatg
     3361 tttctttctg gaactaatat aaaaactatt tcagaaagat taggtcatgc agatataaaa
     3421 ataactatga atagctactc tcagttttta gaagagatgg acagagaagc atttgaaaat
     3481 ttgagcaaaa tactatttat tatattttaa agaagatgac ttattcaata tttgatgcta
     3541 gaatataatt aataaaaatt taattatagg tgattgtatt atgaatatta ttaaaagcat
     3601 aactttactc ttaacattat taattgtgat attaatgaca ggctgttccc caagagaaag
     3661 tgctagtgaa gttgcaaaaa aatatattga taagatgaac tctggtgact atgaaacagc
     3721 atatagttta ttaagtaaag atagtaaaaa aaaaattagt atagaaaatc ttgaagaatt
     3781 tcaaaatatt ttagttagta caaaaagaat aattagttat aaaataggta aagaaaaaat
     3841 cataaaaaaa tataaacatg atggaaaaga atataaaaat gttgttaaat tagaagaaac
     3901 ttttaatata aaaaaccttc tttataaaaa agatgattct ctaaaagtaa atagatattt
     3961 tgtagttgaa aataacaaat acaagctatt attgtatgaa agtttcaaaa aagaatttgg
     4021 tattaatagt atggaccttg ctcaacttaa gcttaatgat tttggactaa aatactttaa
     4081 agaagttgac cttatactaa aaaaagctat tgctattaat cctactgatt caaagcttta
     4141 ttatgcccaa gcatgtaatt atatgaattt tgatggattt cctggttatt cagatacaac
     4201 atataatgct ttagattcaa ttaataaatc tttaaagtat ttagataaaa atgattctga
     4261 ctacaaaaaa aatgcaagta atatatataa cttaaaagga gttattctta tgtttcacaa
     4321 aagatttgat gaagcacaac aatatttaaa taaagcctta tactttaaca aagataatga
     4381 agacccaaaa tataatctaa atacaataaa aaaacatcta aactagatat ttaatttttg
     4441 aatttataaa atattttttt atgtataatt cagcactgaa tcataatttc tactaaacta
     4501 attacaagtt taaattataa agacttattt ctatataaat attactctat aacaataagt
     4561 ttcttattta aattttaaaa tattatctaa aaatcataaa aaagaaggta taaagagcta
     4621 ccctcaagct caatatacct tataaatatc taaacttcat tttttactaa tttttctata
     4681 tctttgataa tatgttcttt agattttttt ccagctatgg catcaaaaat tgcaacagcc
     4741 tttttctctc caaacttttc tgctacatca ttaaagtcta aaaatctaac actttctttt
     4801 ggtttaccaa acatataaca cctctcatac atttttatat atttattata acttctatag
     4861 acttattttt caccaataaa cgttagacaa attagtccct ttattttttt aaactagctt
     4921 taccttcaaa attcttaaca aaaacagttt attctactct ataaataaaa atagacaatt
     4981 taatttgtag gaaactgata atagaataca gtaaataaaa ctttgtttaa tctatctgaa
     5041 aaagttatct attaaaactg ctatgaattt ataaagaata ttctagttca taaaaataca
     5101 aatatgtaaa tataatcacc ttaatattca atttatttaa aataaaaaat gtataaaaga
     5161 tttaataatg acaatatact attctcaata caatctatga tgtaaataat ttaaaactac
     5221 tatattattt aaaaatttat atttatatat gcaattctaa aaacttaata agaagtgaac
     5281 ttagaaaata aagcttaatt tcaaagactt aaatagacta ttattattag tctaaataaa
     5341 ctacgtctat caaaattata tattaaattc ttctaaaatt tttattgcaa atcttgcgtt
     5401 tggatatgta atagacccgc caccaattac acctattttt atagcttcta taatttcatc
     5461 tttacttgct ccagaattaa tacaaccctc aatatgataa caaatacact catcacatcg
     5521 gcttaaaatt gaaatagcta gtcccattaa ttccttattt tttttgctta tagccccttc
     5581 tgaataagtc ttatcatcaa gacttccaaa tactctaaaa aactcatctg tttcgcttaa
     5641 ttttttattg taaaatcccc tatattcttt catttctcta gtattcatct tctacctcct
     5701 atgtttttct tataagaata tcacaccgta cttatatttt caatattaaa tacaaatttt
     5761 cattttatgt tcattagcct aatttatatt attttttgaa atctcaataa gttatcatat
     5821 aagtaagatt ttttcagaac taaaatagaa atcttagtaa tttctttatt atctatctca
     5881 gttctgaaat atattattct tatccaaaca ttattatagt gattaattat attagttatt
     5941 tttacctgaa attttataca tcaaataaga atatataaat ggtagtatta caattacaaa
     6001 gattacaaag atattataaa aaggaatatt aataaatgca ttaataatta ataatattcc
     6061 accaataagc caaatccatc cagctaaatg gtgtgtctta ttccaattat cttcatcatt
     6121 aagagtccat ggtaaaatta ttcctactga gtaatttcgt ttacatttag gaagatagtt
     6181 tccaaataaa ataactatta ttcctaaaat taatcctgta taaaaattag actccaaact
     6241 aactcctgtt gaatataaaa ccatcattat ttgtataatt actgaaagta tagcaactcc
     6301 ccattttgat aaattttgta taacatttga aaaattagct ttcttaggct cattctcaag
     6361 tctaaaccat gtatacaaat ggatacatag cattaacaat ggtacaccaa aagctgctat
     6421 aacctttgga gcataattat ctggatttcc ataattatca aaatgaattg caacttcatt
     6481 tggtaatcta ttataaaaat atattgatac aataaatggt ataaaacata ttaaagtaga
     6541 taaaataaat tgtttattta tcgctttttt cattatcttt ttctcccttc tccataaatt
     6601 gtgaaaacca aagcatgacc tcttcgaaaa ctgaaatatt tatttcgtag aaaataaagt
     6661 tcttatactt tgtttcaaat atgagtcctg ccttttttag ttgagataga tgataagaaa
     6721 ttgtagcatt cgtcatatta aatttttcac taatttctcc tgctgacatt ttattctccc
     6781 tcaacataac caatatttct cttcttatag ggtctgaaag agctttgaat gtttctggaa
     6841 accccaacac catcacctcg ctatttagaa tttcatctaa atagaatata acataacatc
     6901 cattttattt caatagctat ttagaaattt ttctaaatag ttatttctaa atatttattg
     6961 aaaattttta tattaagatt ctcaatttac ttaagtcgct tttatttaat cttactttat
     7021 ctaatttttt taacttaaca aaagtgtatt gcaccataat tgttaaagca aaaacctaat
     7081 gattgaggta cttatatata taacaatact tttaatttta aattaaaagt agctaaaccc
     7141 aatttaaaaa gtagttaagt cctatttaaa tagaaaagaa tgaaacgaga aatatactgt
     7201 ataaaagtac ataaaaacat ctactaatta gatacaacta taacagaata tatttattat
     7261 tataataaca aaagaataaa aaaaattaat ttagcataac cttgttaaat atagatttga
     7321 atatgaattt aaagttatgt aaaattaaaa atacaggaat gaaaattcca ttcctgtata
     7381 aaatctaact tttgagtctc agtacaatct tattttgaga caatcttttt tgttttatat
     7441 tttcttatat ttaaaatata acgcatatct tatgcaacat attcatattt ttctttatat
     7501 tctttcatcg catcattcca agcgtcttgt actgttacac ctcttttatt agcaattttt
     7561 tgtgccagtt caattatctt catagctctt cttgatatgt tcataaacat tcctcctaca
     7621 aattaccata tatttcatat atataaatca aggttatatt tttatgatat ataatccttc
     7681 tattgtaaga ttacagttta tatgtgtatt atatatgaac tatattagaa gataattaga
     7741 atttttataa agctatatga ttagattaaa tataaaagct ttacaattac ttataaattt
     7801 aaaaagtcta taagctaaac taatgtttga tataccaatt ttacgaccat aaataatgcc
     7861 attactaaaa atattggttt tatcatcttt gaacctttct ttatagcaac tttagcacct
     7921 acataagctc ccattatcat cactaatgca aacactatag caattttata atctacttga
     7981 ccatttatca tgaataataa caatgaagtt atattacttg ttaagtttaa tatttttgta
     8041 tttgcagaag catgtaaaaa atcatatcca tatattttta taaatccaaa agtcaaaaaa
     8101 gttcctgtac ctggaccaaa aaaaccatca taaaaaccca ttataagagc cataagcatc
     8161 cctaatctta aatttttctt attaacagct tcaaaattat cttcttgacc taaattttta
     8221 gaaataaagg tgtaaatagc cactattaaa atcataacca caaccaaaac atttaatatc
     8281 tcttcactta ttgataatac acatctcaca cctagaacac taccaataat agtaaatggt
     8341 actaattttt ttaaaaggtt gttattaatt tttccagact ttgcatatct ataggcacta
     8401 cttatacatc ctgctgatgc tgcaaattta tttgtaccaa tggcaagatg tactggcact
     8461 ccaattgcca taagaacagg catgcttata agtcctcctc ctcctgctat agaatctaca
     8521 aaagcagcta aaaacccccc tatacataaa aaaataactg aaaacataaa attcctccat
     8581 cattctcttt caatttttct tacaatatat attatataat cttatttaat acttattatc
     8641 cagcatattc tttctatatt catttggagt tatactatta aacttcttaa atatttgtga
     8701 aaaataacta ctgttattaa aacctacttt cttacaaata tcactaatac tcatgcttct
     8761 atctaacaga tattttttag attcttctat cctaagaaga tttatatact cagttattga
     8821 aactttattt ctttcattaa aaatagaact caaataattt ggacttataa aaaaaacatc
     8881 tgatatacta ttcaaattta tattttcttt ataatatttt tttatatatt cgtttatatt
     8941 ttcaattaga tttcctttat cttttctcca aaatgctcta ttcttatcaa tacactcttt
     9001 ggaaaattca aataatacat tccataaatc ttcatcactg tttacaagtg atattttttt
     9061 attatattta tttcttaaat aagatataag ttttaaatcc tctaaatctc taagcactgt
     9121 tctagacaat acaaatatca tttcctgaat agtatctcta acatcattat atcttaattt
     9181 gtttgtatct attagattat aaacaccttc taaattcata tttttaattt ctttttcaat
     9241 ataaagttct aattcaaagg gatattcttt tgaagaatta ttgaacatat ctatcggatt
     9301 aataatatac tcttcttcta ataatctact atttgctttt ttaagtgcct cttcaagtct
     9361 tatattttct tttctactct tcaatatctc ctcatctttt atactaagtt gattttcaaa
     9421 aaaattcctc ttagctattt cctgaataaa ttcagacatc attaataaat tttcttttaa
     9481 tacatataat ctacttttag gatttaatgg aaattcattg taaattttgt ttatgtattt
     9541 tatatcaata ccagaactac ttgataattc agataaattt ttatctatat caatagactt
     9601 atatactata aaattaccac attctatata tccaactata ttttcttcta atactatagg
     9661 tatctctaaa ctggaaacac catactcaca atggtacact ttttcaaagt tatcattatt
     9721 tatgctttta acaagttttt tagtacactg ttttatattt ttacaaatat cgcaaaattt
     9781 attttctttc ttagtctcaa atatttcttt atactttaag tcatatacag atattctaag
     9841 cttcaatata ttagatattt gatttgcaag attttcaatt atgtttacat taactaactc
     9901 ctgtatattg ttttcagaaa atactatagg tatttgtctc aaactcgaaa atttatgagg
     9961 aacataagta attaatatta ctaaatcaat tttaccagtg tttataattt catgttctga
    10021 atatggtggg caataaacta tagatgtttc ctttatattg acctttttac catcaattat
    10081 ttgtattcct tctccctgaa tcacatataa aagctgttct tcacttaaat gaaaatgctt
    10141 ttcttggtag tttcccggaa aaaatcttac aattccagca ctaagtctat tgaattgtgt
    10201 ttttgatggt tcgtgtatcc acataacctc tccccaatca aatttttgta cttcattatt
    10261 tgactcacta aaatactcca taagctcacc tcttagtttt tgtctaaata ttttttcttc
    10321 ccttcttata ttaaaataat atcatcatca ttaactttca ttactataaa atcatcatac
    10381 tcttttaata ctcctgtaat tttagttgca cttagtttac tacaattcag ttttttaata
    10441 tattgataaa ttctataatt acttttttat tttgtaaaat cagtgttttc ccaaaataat
    10501 tatatctata aatgaaagta tttttactgc ttacatcaat atatgttatc ataattttcc
    10561 tttttattgc atatataaat aactgtatat aacattgtcc aatactatat agaaatacat
    10621 ctaagaaaga atattattaa attgttccaa ttagtattat ttttcttata gaaataaaaa
    10681 aactgttgga gtcttttaga aaaattattt tttacatacc ttttttgctt agatagtcta
    10741 tcttcacagc tattttgtat atacactctt ctagttcatg attttatttt aatatcttgc
    10801 ttatattgct gaaataattg aattctttta tctatcatat tgtctcttat atcacaaaca
    10861 tattgtttct tatatcacaa aaatgaaagc attcttatat cttcaatata taaacctgct
    10921 ttttttaatc ttgtaataaa tataggatac tctatattct cttcctagaa ataactaata
    10981 ccacttattt ttctattata tagaaagtaa taaatctttt tcttaataaa attcaatagt
    11041 aagtggaata aaattttata tgaataagaa cattataaaa atactttata caatagtcaa
    11101 taaaagatat tagcttcaaa ttatgttaaa gttgtattat ttaaagtgaa aaaacaaata
    11161 aaaaagaaat aatagtataa gtagaaaaat atacttaaaa caaggcgaag cctctcttgt
    11221 atctaaatca agtagagaat taaatctaaa aaagaaaata aatataaaag atgactcctg
    11281 tacattacaa gaatcatctt atggtttcat aatcactcca tggtttttaa ctgtctgata
    11341 taaaggtctc agttcactca tagcatgacc accttttccg attgctgacc ccgtcactta
    11401 tggacaaaat gtccataagt gatagtcctc tcttaaaacc ctctaaatag atatatctca
    11461 agcaccgcat tgaaaccagt tgctggaaac aaactgaagc gttctggaat agtaaaagaa
    11521 attgccacca tatccacgtt actgtcctag ttgttacttt gctaacttcg gagcgatttc
    11581 ctctaacaat acgcagtcaa attctgcctt tttgctaaga aatggcagat ggtctgcatc
    11641 tggtataaaa aaaatctgtt tgtctggcgc cactatttca tcaaaaaatt tcttcgctgg
    11701 aagatagggg ctattatagt cattttctcc catgatgtag tatacaggga cctgatagtc
    11761 aactccataa tttcttatgt cagcttcttc cactaaaaac cgcaatacat caccttgatt
    11821 gtcaatcgca tttggattga aaatggccgt attttttaac tctctcaatg aataatacgg
    11881 ggttgccagt atactgatta tattgcccaa atccatattt atagaaagcc catacttgct
    11941 ttggtatgtt cttactgtca taagagctgc caaatagctt ggaccatatt ttgctggcgg
    12001 atatggtgca atagcttcca gcgccttgat atctttttgc ttccctgcag ctttcaccat
    12061 ttcaagtgtc ttttcatagc ccacgcgctc attgtccacc atattaacgg tctgcgcaat
    12121 cccaatgtac gcgctgtaat cctccggata ggtttgcaca gccatagttc ccagaactgt
    12181 tccccagcta tgacctataa ggataatttt ctccttgttg aactcttgct tcaaatatgc
    12241 tgtcacctca tggacgtctt gcagaacacg ctccgcgctc atggtctgca aaactgctgc
    12301 cggatcgttc gcaaagtatg ttttgcctac gtttcgctga tcccagttca ccacagtaaa
    12361 atcattttcc cactcatact ggtacagatg taaaaatggc atcattgaat taccaggacc
    12421 cccatgaacc caaaggatga tggggttgtc tgcgttttgt ccccggatat ataacgcctg
    12481 ctgtatgccg ccgatctcca ccatttccat gcggtcgatt ccaggagctt ctattttatg
    12541 gcttgtacgt ataatcgtgg gggctatcag acgcaaaccg gcaaacgcca gaacaaaaac
    12601 agcaactagt atgcaaagtg tgattaatat cattttaagt accttctttc atgaattttt
    12661 ttagccatag atatctcctc taacattaaa aaaaatatgt tgctatttta gttttgttac
    12721 tctacctgtc ccattggttt cctttcataa aaaatattaa ttgattctat aatctcatct
    12781 tccaggatat caactcctat ttgtctcata agaaactgta agaattgaaa ccgtttacga
    12841 agtgtaggaa agtccccctg aaatacatct gtatcacacc aaaagttaaa gagcagcaaa
    12901 attacctctg cgcattcatc tgggaactct gtgatgatgc ttctatcctc tatgccttct
    12961 cgtaaaacat ttgctaatat tggtgctagt tttttcaaat tatcctgtgt aaatgccagt
    13021 atgatatgcg gactttctat cgcgcttgta atcatgttac tagattcttt tatcacctca
    13081 tccgttaaat gccgcctaac taaacttcta agcttctctt ttgctgtaag tccatgcatt
    13141 tcaccgagcc attgatttac tgtctctatt atctgttcaa agcgcctttc catgaccaca
    13201 ttaaaaatat cttcttttga actgaagtga tgaaaaattc cgccctttga cattcctgaa
    13261 gcatcaacaa tattttgcat acttgttttg tcatatcctt tttcagcaaa caactttgca
    13321 gaaatggtaa tgatattttc aatagcttgc tttggattgt tctttctagt cattatataa
    13381 ctccttctta ttaaaaaccg actgttggtt tttattatat aagctattcc tgcttctgtc
    13441 aagtaacatt gtgtttatgg caaaacaata aggtttggtg gttagacagg tattaattag
    13501 actaatgcga ctttccccta gatggaaccc ttggtaaatt tgcaatcttt accggctttg
    13561 attatatctg taagcaaggc aggtatggcc acacctgccc tgtttgcccc tcggatattc
    13621 attcctatcc ctcctctcct tagccattac accaaaacgc cgctcattgt ctccaatggc
    13681 ggcgttttaa agatatgatt tctcatgctg ctgaaatatg caaaaaatat aagatgccct
    13741 ttattatttt tcctacaata gaaaagaaaa gtattgaaga atgggttaat attggagcaa
    13801 atatgctttg tttggcacaa gatactacct ttatcactta tgttatcaat caattattat
    13861 ctttttataa tgagattgta ccaattcaaa aacaacatta aattataaat ctaattttat
    13921 attaaaagag actaatattt tatctatctc tattaaggat attttcttat gtatcataat
    13981 attagtctct cttattatta tatactgcta tcatcattag ttgctattta tactattttt
    14041 ataatataca aatttattca gctaacgttt tgcctaattc tatacactca tcttttgctt
    14101 cttcatttgg gtcttccatt gctgtaattc cttctccacc aataattgta gcaccagcat
    14161 tttgcatacg ttcttcccag tctctcatcc attcacaatt tccccatcca tatgaaccaa
    14221 ataatccaat ctgtttacct gataccatag attctaattc tgaaacaaat ggctccatat
    14281 acccctcttc tagttgctct gctcccatag atgggcaacc taatgcaaat ttatcttctt
    14341 cttttaaatc acttggtttc agtaagctca catctaaaac ttctggtgtc ttacctttta
    14401 acttcacacc ttcagccaca aaatttgcca ttttttcagt atttcctgtt ccactccaat
    14461 aaacaatata tattttactc atttttacct cctgtagtat tttttaatta atcaatagtt
    14521 agttatagct aactaacatc taaaaaacaa aagatttctt tatctgaaat ctctttattt
    14581 ttctaaatat agaatatcat ggttgatttt gaaattcaat acttattgat aatatttatc
    14641 atttatattt tatattcatt ttataatatc aaaacactgt atcgtatttc taatctttca
    14701 tagaaaaatt tctaacttat agtacttttg catctcttat aaatctttta tatgtataag
    14761 tacatagaaa aacacacctt ttccagatgt gctatctata atatcgtata tttatttttc
    14821 aattctaact agctaatatg tttataaatt ttatcactta atacctttat acattgatgt
    14881 aaaatcttac acctaagtaa tttttattat ttgtctcgac tctatataat atagctattt
    14941 tatcatcaaa tcaaaaatta tcttttgcta ttattcttgt atttataaag atttaaatat
    15001 aagtatccat ttccttgttc cgttctaggt attccaagcg ctctcgtaca ttttattaat
    15061 tgtgcataca gttcagcttc atctaaatct ctttcaaact cctctcttgc ccattcttta
    15121 attagtgcta atgagcctga tacgtatggt gcactcatac tggtaccact cataatagca
    15181 agtttattat ccatataagt cgatataata tttcttcctg gagctaccaa atctattgta
    15241 gtatttgaat tactaaactt ttcaaccaaa tagttctcat ttattgcacc tacttctatt
    15301 acctcagcat aactggctgg ataactatac tcacttgtac tagaatcacc atctccatta
    15361 ttacctgctg cacacactac agaaatatta ttttttactg cttgcatgac agcatttttt
    15421 aaattcttat catctttgtt tcccccaaga gacatagata taatatcaac cttgttattt
    15481 acagcaaagt taatagcatt aattatactt tgatatgttc cagtaccatc tttatttaat
    15541 gcttttgcta ttaataattt acaatctgga gctactccca taacttcatt attataatta
    15601 gatgcagcta taatacccgc cacatgagtt ccatgaccat taaaatcctc atatatattt
    15661 ttatttccat tactgtcatc actaaaattt gcaccaccaa ttatttttcc ttttaaaaga
    15721 ggatgagata tatcacaacc tgtatctatt ataccaacta caatattttt accagtataa
    15781 ccttcatccc acattcccct agcattcatt tgttttattc catatgggaa tttatttttt
    15841 gcacctctta gattttcatt tatttcatat ggaattagtt tcattctttt attcatcata
    15901 tatcctccta aaaattttat tgcttataaa aatcatgaat tttattttat aataggttta
    15961 taaatcaaat taaaacctat atctacttat ttatatttat gatatatttt
//
LOCUS       DAYATN010000085        15978 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.085, whole genome shotgun sequence.
ACCESSION   DAYATN010000085 DAYATN010000000
VERSION     DAYATN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.085"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="ACGI8A_002484"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="ACGI8A_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFH0180476.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="ACGI8A_002485"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="ACGI8A_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFH0180477.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="ACGI8A_002486"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="ACGI8A_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFH0180478.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="ACGI8A_002487"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="ACGI8A_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFH0180479.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="ACGI8A_002488"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="ACGI8A_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFH0180480.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /gene="rplJ"
                     /locus_tag="ACGI8A_002489"
     CDS             2662..3168
                     /gene="rplJ"
                     /locus_tag="ACGI8A_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFH0180481.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="ACGI8A_002490"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="ACGI8A_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFH0180482.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3982..4770
                     /locus_tag="ACGI8A_002491"
     CDS             3982..4770
                     /locus_tag="ACGI8A_002491"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFH0180483.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5241..8957
                     /gene="rpoB"
                     /locus_tag="ACGI8A_002492"
     CDS             5241..8957
                     /gene="rpoB"
                     /locus_tag="ACGI8A_002492"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFH0180484.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            8999..12484
                     /gene="rpoC"
                     /locus_tag="ACGI8A_002493"
     CDS             8999..12484
                     /gene="rpoC"
                     /locus_tag="ACGI8A_002493"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFH0180485.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12773..13195
                     /gene="rpsL"
                     /locus_tag="ACGI8A_002494"
     CDS             12773..13195
                     /gene="rpsL"
                     /locus_tag="ACGI8A_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFH0180486.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13319..13789
                     /gene="rpsG"
                     /locus_tag="ACGI8A_002495"
     CDS             13319..13789
                     /gene="rpsG"
                     /locus_tag="ACGI8A_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFH0180487.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13838..15904
                     /gene="fusA"
                     /locus_tag="ACGI8A_002496"
     CDS             13838..15904
                     /gene="fusA"
                     /locus_tag="ACGI8A_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFH0180488.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6257 a         1990 c         3249 g         4482 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa ataatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgaaag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctgtata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga gtgtttataa ataaaatgta ataaataatt aattaacaaa
     3901 tataaatttt aaaataagtt agatatttag aaaaatcgaa atttgtagga tttaaaggac
     3961 atattcaagg aggagttaat aatggaaaaa ttacaaggaa aaattgcagt agttactgca
     4021 gcaacaaaag gtattggatt agcatcagca gagatattag caaaaaatgg agcaactgtg
     4081 tacttagcag ctcgttcaga agaattagct aatgaagtta taaataaaat aagtgcagaa
     4141 ggtggttgtg ctaagtttgt ttactttaat gctcgtgaag aagagacctt tacctcaatg
     4201 atagaagagg tagttaaaaa agaaggtaag atagatatac ttgtaaacaa ttttggttca
     4261 acaaaccctt ctcttgacaa agaccttgta actggggata cagataattt ctttgacaca
     4321 gtaaatacta atttaaaaag tgtgtattta ccatgtaaag cagcaattcc tcatatgata
     4381 aagaatggaa aaggaagtat agtaaatata tcaagtatag gttcagtatt acctgattta
     4441 tctagaatag cttactgtgt atcaaaagca gcaatcaact cattaactca aaacatagct
     4501 acacaatatg ctaaagacaa tgttagatgt aatgctgtac ttccagggct tattgcaact
     4561 aaagcagcat tagacaatat gtctccagag ttcataaaag aatttttaaa gcatgttcct
     4621 ttaaatcgta taggggaacc agatgatata gcaaaagctg ttctattcta tgctagtgat
     4681 gactcatcat ttataacagg ggatttactt gaagttgcag gaggatttgg tttacctact
     4741 ccacaatttg cagataatat attaggataa ttaaattact aatatgcaat gaaagcttat
     4801 gcattagtta tagtagaaat gatattttta taaaggccat agatataatt tatattggaa
     4861 aggtttgagg ttcaaaattc caagttaaat ttatttatgg tctttatttt gtatatttta
     4921 ataaattgta tatatttgta taaatgtaat tatttacaat cattttaata tggaattata
     4981 attaaatagg ggggatggta cttgtataaa attttttata gaaaattaga cctataaatg
     5041 aaaaatatgg attttatatt ggaaaattgt acataataca taatatatgc ttaaaataat
     5101 ttacaaaaaa tcaaaaataa tgcatagttt acatataaga taaaaaatat attgaaaaat
     5161 acttgacatt atgctaaaat tattagatgc gttagaatga actattgttt tatttttaat
     5221 tattgagagg tgaaaagtga atgccacatc ctgtcacgat aggtaaaaga actagaatga
     5281 gcttttctaa aattaaggaa atagctgatg tgccaaatct tatagaaatt caagtagatt
     5341 cctatgagtg gtttttaaaa gaaggtttaa aagaagtgtt tgatgatatt tcaccaatag
     5401 aagattatac tggtaatctt atattggaat ttgtcgacta ttctctagat gataaaccaa
     5461 agtacgatat agaagagtgt aaagagagag atgctacata ttgtgcacct ctaaaggtta
     5521 aggtaagact tataaataaa gaaacaggtg agataaaaga acaagaagtg tttatgggtg
     5581 acttcccttt aatgacagaa agaggaactt tcgttataaa tggagcagaa agagttatag
     5641 taagtcaatt agttagatct cctggtgttt actatgcgga agaaagagat aaaacaggta
     5701 agagattgat ttcatctact gtaattccta atagaggagc atggttagaa tatgaaacag
     5761 attctaatga tgtaatatct gtaagagttg atagaacaag aaaacaacca gttacagttt
     5821 tacttagagc tttaggaata ggaacagatg cagagataat tgacctttta ggagaagatg
     5881 agagattatc tgctacatta gaaaaagata atactaagac agtagaagaa ggtcttgtag
     5941 agatatacaa aaaattaaga ccaggtgaac ctcctactgt agaaagtgca tcatctttat
     6001 taaatgcttt attctttgac ccaaaaagat atgacctagc aaaagttggt agatataaat
     6061 tcaataagaa acttgcttta tgttatagaa taatgaataa aatttcagca gaagacataa
     6121 taaatccaga aactggagaa gtatttgtaa aagctggaga aaaaataagc tatgatttgg
     6181 ctaaagccat acaaaatgca ggtataaatg tagtaaatct actaatggat gatgataaaa
     6241 aagttagagt tataggaaat aattttgtgg atattaagtc tcacattgat tttgacatag
     6301 atgatttaaa cataaaagaa aaagtacatt atccaacttt aaaagaaatt ttagatggat
     6361 atagtgatga agaagaaata aaagaggcta taaaatcaag aattaaagaa cttataccaa
     6421 aacatatatt attagatgat ataatagctt caataagtta tgagttcaat atattctata
     6481 atataggaaa tattgatgat atagatcacc taggaaacag aagaataaga tctgtaggtg
     6541 aattgttaca aaatcaagtt agaattggtc tttcaagaat ggaaagagtt ataaaagaaa
     6601 gaatgacggt tcaagatatg gaagctataa cgcctcaagc gttagttaac ataagaccag
     6661 tttcagctgc aataaaagaa ttttttggta gttctcagtt atctcagttc atggatcaaa
     6721 caaatcctct atctgagtta acgcataaga gaagattatc agcccttggg cctggaggtc
     6781 tttcaagaga aagagctgga ttcgaagtgc gtgacgttca ccattcacac tatggtagaa
     6841 tgtgtccaat agagactcca gagggaccaa atatcggtct tataaactct ctaggaactt
     6901 atgcaaaaat aaatgaattt ggatttatag aatcaccata tagaaaattt gataaagaaa
     6961 catcaacagt tactgatgaa atacattatt taactgctga cgaagaagat ttattcgtaa
     7021 gagctcaggc aaatgagcca ttaacagaag atggtaaatt cgtaaaccat agagttgttt
     7081 gtagaactgt aaatggtgct gttgaaatgg ttcctgaaag tagagtagat tacatggata
     7141 tatctcctaa gcaggttgta tctgttgcca ctgctatgat acctttctta gaaaatgatg
     7201 atgccaaccg tgcccttatg ggagcgaaca tgcagcgtca ggcagtgcct ctagttagaa
     7261 gagaagcacc aattatagga actggtatag aatacagagc agcaaaagac tctggagcag
     7321 ttgttgttgc tagaaattct ggtatagcag aaagagtaac agcagatgaa ataattataa
     7381 aaagagaaga tggaaacaga gatagatata atctacttaa gtttaaacgt tcaaactcag
     7441 gtacttgtat aaatcaaaca cctataataa ataagggtga ccagataata aaaggtgatg
     7501 ttatagcaga tggtccagca actgatttag gagaagttgc acttggaaga aactgtctta
     7561 tagcattcat gacttgggaa ggttataact acgaggatgc tatattaata aatgaaagat
     7621 tagttaaaga agatagatta tcaacaattc atatagaaga atatgaatgt gaagctagag
     7681 atacaaaact agggccagag gaaataacta gagatatacc taatgttgga gatagtgcaa
     7741 ttaagaactt agatgacaga ggtataataa gaataggtgc agaagtagac tcaggtgata
     7801 tattagttgg taaagtaact ccaaaaggag aaactgaact tactgctgag gaaagattac
     7861 ttcgtgcaat atttggagaa aaggctagag aagttagaga tacttcactt aaagtgcctc
     7921 atggtgaatc tggtataata gttgatgtaa aagtatttac tagagaaaat ggagatgatt
     7981 tatctccagg agtaaatgaa ttagtaagat gctatatagc taaaaagaga aaaataaaag
     8041 ttggagataa aatggctggt cgtcatggta ataaaggggt tatctcaaga gtattacctg
     8101 aagaagatat gccattcatg gaaaatggaa caccactaga cataatactt aacccacaag
     8161 gtataccatc acgtatgaac attggtcagg ttttagaagt tcatttagga cttgctgcta
     8221 agacattagg atggtatgta gcaacatcag tattcgatgg tgctaatgag tatgacataa
     8281 tggatgctct tgaagaagct gggtatccta gagatggtaa attaacttta tatgatggta
     8341 gaacaggtga gtcttttgac aacagaataa ctgttggata tatgtactac ttgaaactac
     8401 atcacttagt agatgaaaaa ctacatgcaa gaagtactgg accatactca ttagttactc
     8461 aacagccact aggtggtaaa gcacaatttg gtggacaaag atttggagag atggaggttt
     8521 gggctctgga agcatatgga gctgctcaca tacttcaaga aatacttact gttaagtctg
     8581 atgacgttgt aggtcgtgtt agaacatatg aagctatagt taaaggtgaa aatatacctg
     8641 aaccaggaat cccagaatca tttaaggttc ttataaaaga acttcaaagt ttatgcttag
     8701 atgtaaaagt attgacagat gaagaccaag aaatagaagt aagagaatct gtggatgaag
     8761 atgatacaat aggtgaattt gagctagatg ttgtaaatca tatgggagaa gttgaagaaa
     8821 gtaatataat agaagaaatt gaagatgatt ttgcagaaaa tgcagaagat gaggatatag
     8881 agaatttaga agaattcact gaagatgatt tatttgaaga agaaatagat tttgatagtg
     8941 atgactttga tatgtaaatt taattaatgt aaatctcaaa tagaagggag agaactcctt
     9001 gtttgaatta aacaatttcg agtcgataaa aatagcattg gcttctccag aaaaaataag
     9061 acaatggtct aggggagaag ttaaaaagcc agaaactata aattaccgta ctttaaaacc
     9121 agaaaaagat ggtcttttct gtgaaagaat atttggacca caaaaagact gggagtgcca
     9181 ctgtggtaaa tatagaagag ttagatataa aggtgtagtt tgtgatagat gtggagtaga
     9241 agtaactaaa tcaaaagtaa gaagagagag aatgggacat atagagctag ctgctcctat
     9301 gtctcacatc tggtacttca aaggtatacc aagtagaatg ggacttttac ttgatatgtc
     9361 accaagatcg ttagaaaaaa tattatactt tgcctcatat gtggtagttg atccaggaga
     9421 gactggatta aacgaaaaac aattgcttac agaaaaagaa tacagaactg ctcttgaaaa
     9481 gtatggatat acttttactg taggaatggg tgctgaagct gtaaaaacat tactacaaaa
     9541 tatagattta gagcaacaaa gtaaagacct aagagcggag ttaaaagata gtacagggca
     9601 aaagaaagtt agaacaataa gaagattaga agttgtagag gcatttaaaa agtctggaaa
     9661 taaaccagaa tggatgattt tagatgcaat accagtaata ccaccagatt taagaccaat
     9721 ggtacaactt gatggtggaa gatttgcgac ttcagaccta aatgatttat atagaagagt
     9781 tataaataga aacaacagac ttaaaagatt attagagctt ggagctccag atataattgt
     9841 aagaaatgaa aaaagaatgc ttcaagaagc tgttgatgca ttgatagata atggtagaag
     9901 aggtagacct gtaacaggac ctggaaatag accacttaaa tctttatcag atatgttaaa
     9961 aggtaagcaa ggtcgtttcc gtcaaaactt acttggtaag cgtgttgact actcaggacg
    10021 ttctgttata gttgttggac cagaacttaa attttatcaa tgtggacttc caaagaagat
    10081 ggcattagaa ttattcaagc catttgttat ggataaatta gttaaagaag gttatgcaca
    10141 taatataaaa agtgcgaaat ctatagtaga aaaagttaag ccagaagttt gggatgtttt
    10201 agaagatgtt ataaaaagtc atccagttct tcttaaccgt gcaccgactc tacatagatt
    10261 aggtatacaa gcatttgaac caatcttagt tgaaggtaag gctataaaac tacatcctct
    10321 tgtatgtaca gcttataatg cagactttga tggtgaccaa atggcagttc atgtaccttt
    10381 atcagtagaa gcacaagcag aagcaagatt cttaatgctt tctgtaaata atatacttgc
    10441 tcctaaagat ggttcaccta taactactcc atctcaggat atggttttag gttgctatta
    10501 tctaacaata gaagcccaag atggagctaa aggaacaggt atggtattta aagactttaa
    10561 tgaactatta cttgcttatt acaataaatc agttcatcta catgcattag tgaaacttaa
    10621 ggtaacactt gaagatggaa gaagttcatt ggttgaaagt actgttggta gatttatatt
    10681 taatgaaaat atacctcaag acttaggttt tgtagataga aaagaaaatc catttgcact
    10741 tgaagttgat ttcttagcag ataaaaaatc tcttggtaaa ataatagata aatgctttag
    10801 aaaacacgga aatacagaaa cagctgaatt actagattat ataaaagctc taggatttaa
    10861 atattctaca ttaggtggca taacagttgc tgttgatgat atgagtgtcc cagaagaaaa
    10921 gaaagtattt atagctgagg cagaagctaa agttgataag tatgaaaaag catacagaag
    10981 aggtctaatc tctgatgaag aaagatatga aaaagttata gagacatgga cagaaacaac
    11041 tgataaagtt actgatgctc ttatgggtgg actagataga ttaaataata tatatataat
    11101 ggcacattca ggagccagag gttctaaaaa ccaaattaga cagctggcag gtatgcgtgg
    11161 tcttatggca aatgcatctg gtaaaacagt tgagatacca gttaaatcta atttccgtga
    11221 aggtttatca gtactagaat actttacatc ttcacatggt gccagaaaag gtcttgccga
    11281 tacagctata cgtacagctg aatctggata tttaacaaga agacttgttg atgtaagtca
    11341 agatgttatt gtaagggaaa tagattgtgg aacagaagat actacagaga tttatgctat
    11401 aaaagaagga aatgaagtta tagaagagat atatgataga attgtaggaa gatatactat
    11461 agaccctata ttaaatcctg aaactggaga agttatagtt gaggctgatt caatgataca
    11521 agaagatgaa gcagaaacta tagtagcttt aggaattgaa aaaattagga taagaacagt
    11581 tcttaactgt aaaactaatc atggagtttg ttctaagtgt tatggtagaa acttggcaac
    11641 aggaaaagaa gttaatatag gtgaagcagt tggtataata gcagctcaat ctatcggtga
    11701 gccgggtact cagcttacaa tgcgtacatt ccatactgga ggagttgcag gagctgatat
    11761 aactcaaggt ctaccaaggg ttgaagaatt atttgaagct agaaaaccaa aaggattagc
    11821 tgtaataact gaagtatctg gtagagttga aattgatgaa acaggaaaga gaaaagaagt
    11881 aaatgtaata ccagaagaag gcgagactca aacatatgta attccatatg gttctagatt
    11941 aaaagttaag caaggtcaaa tgctagaggc tggagaccct ctaacacaag gatttataaa
    12001 tcctcatgat atagtaagag taaatggtgt taagggagtt caagaatata tagttaaaga
    12061 agttcaaaga gtgtatagac ttcaaggggt tgatgttaat gataagcata tagaagttat
    12121 agtaagacaa atgctatcta aagtaaaagt tgaagaccca ggagatacag atttattgcc
    12181 aggtggatat gaagatgtat taacattcaa tgaatgtaat aaagatgcta tagataaagg
    12241 tctaagacca gcagttgcta aaagagtttt acttggtata actaaagcat ctcttgcaac
    12301 tgattcattc ttatcagcag cttctttcca agaaacaaca agagtattaa cagaggcagc
    12361 aataaaaggt aaagaagacc acttaatagg acttaaagaa aatgttatat taggtaagtt
    12421 aataccagca ggaacaggaa tgaagaaata tagaaatata gctgttgaaa aaattgaaga
    12481 ttaatagcat atagagtaaa gaaatcttga caaccattat ttgtggtgtt aaaatagtaa
    12541 agtgtgttgt ttaaaagtac actaagggct tgaacaagtt cttagtgact tttagatggc
    12601 aataaatgag aactaaaaag catcaagcta gaaaagtgct aatatcacat atttatgtgt
    12661 tatagacatt aaattgtaag taacgaatgt tacaatcagt cagtatgaat attcatattg
    12721 atgtagttgg cggtcactga taagatcgaa aatttggaag gaggtgcaga tgatgccaac
    12781 aattaaccaa ttagttcgta aaagtagaaa agcgctagag aagaaatcta ctgctccagc
    12841 attacaaaaa ggttacaact ctttaaataa gaaagtaact gacgctagtg ccccacaaaa
    12901 aagaggggtt tgtacttcag taaaaacagt tactcctaag aagcctaact cagctttaag
    12961 aaaagttgcc agagttagat taactaatgg tatagaagtt tctgcttata taccaggaga
    13021 aggacacaac ttacaagagc atagtgttgt tcttataaga ggaggaagag taaaagacct
    13081 tccaggggtt agataccata tactaagagg tactctagat acagcaggtg ttgataagag
    13141 aagacaatca agatctaaat acggtgcgaa gagacctaaa gaagctaaaa agtaaaaaca
    13201 attaatgatt gattatagtt cggtgcttta aatatatata tatttacggc atcacggcat
    13261 taaatgattt atgtcgagta cctatgattt aagttaaata attaaggagg gaagcgaaat
    13321 gccaagaaaa ggtaatattc caaaaagaga agttttacca gatcctatgt atgggagtaa
    13381 ggtagttact aaattaataa ataatctaat ggtagacgga aaaaaaggaa agtctcaaag
    13441 aatagtttat gatgctttcg caattgttgc tgaaaagaca ggagaagaag ctttagaagt
    13501 atttaataaa gcaatggata atataatgcc tgttttagaa gtaaaagcaa gaagagttgg
    13561 tggggcaaac taccaagtgc caatcgaagt tagacctgaa agaagacaaa cattaggttt
    13621 aagatggcta gtaaaatata ctagagctcg tggagaaaaa ggaatggttg aaaagctagc
    13681 taaagagata atggacgcag ctaacaacac tggagcttca gttaagaaaa aagaagatac
    13741 ccataaaatg gcagaagcta ataaagcatt tgctcattac agatggtagg atatatagat
    13801 atcttataaa attgaggata tcataaggag gataaccatg gctagaaagt ttcctttgga
    13861 aagaactagg aatataggaa tcatggctca tatagatgca ggaaaaacta ctactacaga
    13921 aagaatcctg ttttatactg ggcaaactca taaaatagga gaaactcatg aaggagcttc
    13981 tcaaatggac tggatggagc aagaaaagga aagaggtata acaataactt ctgccgctac
    14041 tactgcttca tggaaagacc atagaataaa cataatagat actcctggac acgtggactt
    14101 cacagttgag gttgaaagat ctctaagagt tcttgacggt tcagttgctg tattctgtgc
    14161 aaaaggtggg gttgagcctc aatcagaaaa tgtatggaga caagctgaaa cttatggagt
    14221 acctagaata gcttttgtaa ataaaatgga tatattaggt gcagatttct acaatgttgt
    14281 aagcatgatg aaatctagat taaattctaa tgcagttcct atgcaattac caataggtaa
    14341 agaagattca tttataggta taatagactt attaaaaatg gatgctgtta tatacaaaga
    14401 cgacctaggg gttgaaatgg aagaaacaga tataccagaa gatatgaaag aattagctgc
    14461 tgagtggaga gaaaaattag ttgaatcagt agctgaaact gatgaagaat taatgatgaa
    14521 ataccttgaa ggtgaagaac ttactataga tgaattaaaa gttgctataa gaaaagctac
    14581 tatagcttgt gaaatgaacc cagttttctg tggtacagct tatagaaaca aaggtgttca
    14641 gttagtaata gatgcagttt tagattactt accagcgcca actgatatac cagctataaa
    14701 aggtatatta gcagatggag aagaagctga aagacattct tctgatgaag agccattctc
    14761 tgcattagca ttcaaaataa tgactgaccc attcgttgga aaactagcgt tcttcagagt
    14821 ttattctgga acattagaat caggttctta tgttttaaat gcaactaaga ataagagaga
    14881 aagaattggt cgtatattac aaatgcatgc taatacaaga gaagaaataa ctaaagtgta
    14941 tgctggagat atagcagcag cagtaggatt aaaagatact actactggag acacattatg
    15001 tgaccctgct aatccaataa tacttgaatc tatggaattc cctgagccag ttatatctgt
    15061 tgctatagaa ccaagttcta aagcagctca agaaaaaatg ggtatagctc ttcaaaaatt
    15121 agctgaagaa gacccaactt tcacagttaa gactgaccaa gaaacaggac aaactataat
    15181 atctggtatg ggtgaattac acttagagat aatagtagat agattattaa gagaatttaa
    15241 agtagaagct aaagttggtg caccacaagt tgcatataga gagactataa ctcaaccagt
    15301 tgatgttgaa tacaagtact ctaagcaatc aggtggtaga ggacaatacg gtcacgttaa
    15361 gattagagtt gctcctcaag agccaggtga aggatacaaa ttcacaaaca aaactgttgg
    15421 tggatcagta ccaaaagaat atgttggtcc agttgacatg ggtatacaag gagctatgca
    15481 atctggtata gttgctggat atccagttgt agacgttgct gttgagttat atgatggttc
    15541 ttaccatgag gttgactctt ctgagatggc gtttaagatg gctggttcaa tggcattcaa
    15601 agatgctatg aagaaaggta atgcagttct tttagagcct tacttcaaag tagaagttgt
    15661 tactccagaa gattacatgg gagatgttat gggtgactta aactcaagaa gaggattaat
    15721 ccaaggtatg gaagctagaa gtggtgcaca agttataaat gcattcgttc cactttcaga
    15781 aatgtttgga tactcaacag acctaagatc ttcaactcaa ggtcgtgcaa cttacacaat
    15841 gatattcgat cactatgagc aagttccagc ttcagttgct aagaaaatag ctgaaggaaa
    15901 ataatttaaa aataattaat aatataaata taaaaaataa tcagctagat gagagatatc
    15961 tctcatctag cacaagat
//
LOCUS       DAYATN010000086        15854 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.086, whole genome shotgun sequence.
ACCESSION   DAYATN010000086 DAYATN010000000
VERSION     DAYATN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.086"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            262..1179
                     /locus_tag="ACGI8A_002497"
     CDS             262..1179
                     /locus_tag="ACGI8A_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFH0180489.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(1308..2237)
                     /gene="pfkB"
                     /locus_tag="ACGI8A_002498"
     CDS             complement(1308..2237)
                     /gene="pfkB"
                     /locus_tag="ACGI8A_002498"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFH0180490.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            2506..3231
                     /locus_tag="ACGI8A_002499"
     CDS             2506..3231
                     /locus_tag="ACGI8A_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0180491.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            3314..4468
                     /gene="nagA"
                     /locus_tag="ACGI8A_002500"
     CDS             3314..4468
                     /gene="nagA"
                     /locus_tag="ACGI8A_002500"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFH0180492.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(5177..5647)
                     /locus_tag="ACGI8A_002501"
     CDS             complement(5177..5647)
                     /locus_tag="ACGI8A_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFH0180493.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILLFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIY"
     gene            complement(6251..7408)
                     /locus_tag="ACGI8A_002502"
     CDS             complement(6251..7408)
                     /locus_tag="ACGI8A_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFH0180494.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            7562..8131
                     /locus_tag="ACGI8A_002503"
     CDS             7562..8131
                     /locus_tag="ACGI8A_002503"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFH0180495.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            complement(8313..8729)
                     /locus_tag="ACGI8A_002504"
     CDS             complement(8313..8729)
                     /locus_tag="ACGI8A_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180496.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(8955..10049)
                     /locus_tag="ACGI8A_002505"
     CDS             complement(8955..10049)
                     /locus_tag="ACGI8A_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180497.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            complement(10472..11413)
                     /locus_tag="ACGI8A_002506"
     CDS             complement(10472..11413)
                     /locus_tag="ACGI8A_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HFH0180498.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
     gene            complement(11406..12233)
                     /locus_tag="ACGI8A_002507"
     CDS             complement(11406..12233)
                     /locus_tag="ACGI8A_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFH0180499.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(12404..12787)
                     /locus_tag="ACGI8A_002508"
     CDS             complement(12404..12787)
                     /locus_tag="ACGI8A_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFH0180500.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            complement(12756..13043)
                     /locus_tag="ACGI8A_002509"
     CDS             complement(12756..13043)
                     /locus_tag="ACGI8A_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HFH0180501.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(13076..14233)
                     /gene="alr"
                     /locus_tag="ACGI8A_002510"
     CDS             complement(13076..14233)
                     /gene="alr"
                     /locus_tag="ACGI8A_002510"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFH0180502.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEKSVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(14248..14838)
                     /gene="gerS"
                     /locus_tag="ACGI8A_002511"
     CDS             complement(14248..14838)
                     /gene="gerS"
                     /locus_tag="ACGI8A_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="germination lipoprotein GerS"
                     /protein_id="HFH0180503.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQDDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(14859..15320)
                     /locus_tag="ACGI8A_002512"
     CDS             complement(14859..15320)
                     /locus_tag="ACGI8A_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFH0180504.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(15335..15715)
                     /gene="acpS"
                     /locus_tag="ACGI8A_002513"
     CDS             complement(15335..15715)
                     /gene="acpS"
                     /locus_tag="ACGI8A_002513"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFH0180505.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
BASE COUNT         5375 a         2604 c         1841 g         6034 t
ORIGIN      
        1 ttttacgata aaagcttgtt cagaccaaag tggtaattac cactaatcca aatataaatt
       61 aatttgaatt ttttgtcaat acataaaata tttatatcaa tcaattttat ggatatccta
      121 caaaattact ttatcaatat ttcttatttc gctaatttac ttatataagc tttaaattta
      181 ttttttgata caaagtttac aaatttatgt gtctgaatta taatcaattc tgtaaatata
      241 aaaaccttag gagaaagtat tatgaaaaat ataaaaatgg gaatgatagg tcttggctcc
      301 atagctcaaa aggcatattt acctatatta actaagtctg agagatttga gtttgtagga
      361 gcttttactc caaataaagt taaaagggaa aaagtctgta gtgattatag aatcatgcca
      421 ttcgattcta ttgaatccct agctaaaaaa tgtgattgta tttttctaca tagctctaca
      481 gaaacacact acgaaattat aaaaatatta ttaaatttag gtgttcatgt atatgtagac
      541 aaacctcttg cttcaacagt aagtcaagga gaagaactta tcgagttaag tacgaaaaaa
      601 aacttaaatt taatggttgg attcaaccgt agattctgtc ctatgtacaa agaaattaaa
      661 aataatgcta cagaaattgt atctatcaat atatgtaaac atggattaaa ttccttaaga
      721 aatgttagat tcgacagtac tcttatagat gactatatac atgttattga cactgcttta
      781 tggttagcaa atgaagatgt tgaaattagt ggagaggatt tatttttaac agacaataag
      841 aatcttatat ttgtaagtca taaacttaaa ggtaaaaatt tctctataaa cacctctatg
      901 catagagatt ctggaacaaa gttagaacaa gttgaaattc tttcaaaggg taagattcaa
      961 agagttaaga atcttaatgt actggaaata gaagaaggtg gaaacctaac tttaaagcaa
     1021 agtggtgcat gggtaaatat attaaaacaa aagggttttg aggatatatc aaatcatttt
     1081 attgattgta tagaaaataa tataaaacca gctattaatg gagaagagtg tatcaaagct
     1141 caaagattac tagagaaaat tataaattca gtaaaataga tatttattat aaaaataggt
     1201 taaaaatgaa tttttctaac ctatttttat ataagactat atcttttata ttaaaattaa
     1261 aatctattac aacaacatta aatttattat attcccatta aatgtattta acttaacttt
     1321 tttacttcaa ctaaatcttg atatttatca atttctccca aatctacaaa tccagtttcc
     1381 atatttaaag cattacttat accacaagca tttgcaaatt taagtaaatt ttctatttta
     1441 taacccctta aaatacctac actaaatcca gccactgtgc tatctccact acctacagta
     1501 tttatacact ttacaactgg tacttttaca tcatatacat tatcttcgtt taaatacacc
     1561 ataccatctt ttcctaaaga tatacataca ttctctgcac ccatttcaat aagttttttt
     1621 cctgacaaga taatttcctc tcttgattct atattcattc ctaataaatg tcttatttca
     1681 tctatatttg gttttattaa atatggtttt gattttaaag ctatttttaa tgcttctcct
     1741 gatgtatcta atataaactt tatattattt gctctacata tatttcctat cttttcataa
     1801 tagtctatcg gcatattctc acaataacta ccagaagcta ccagtatttt agtatttttt
     1861 agtacttcct ctatatcatt ttcaaatctc tctaaatcgc ttttttctac tattggtcct
     1921 ttttctaaaa attctgtact aactttattg tcctcaatta tgtttaagca atttctagtt
     1981 tcctgctcta tttttgtaaa tttattttgt atatctagtt ttctaagttc attttttata
     2041 aattctccat taaatccgcc tagaaatccc atagctaaag gattttcttt taaaatttta
     2101 cataccttag ctacgtttaa tcctttacct ccagcagttg cattcttact tactaccctt
     2161 tgcacctcac ctatgtttat attattcact ctatacattc tatctataga tgggttgaat
     2221 gttattactg taatcatact agattctcct cattattaat ttttactttt ctaaacaata
     2281 aattaatctg taaaataaaa aatacctcat ctataatata cccaaagtgg ttattaccaa
     2341 tttgaatata aattatttta cttgttttgt caatgtacca taattttata ataaattaat
     2401 aaccttactt attgaatttt gattaaactt tattcattga cataaataat tctatattct
     2461 ataatcaaat tgtggtaata accacttaga aaggactata tttttatgat aaaaagaaat
     2521 gataaaagac caatatatga ccagcttgtt gagatattaa gaactaagat tgaaaatgag
     2581 atggaaccaa acgatagaat gctttccgaa agaaaaattt gtgatgaata tggagtaagt
     2641 agaaccacag ttagactagc tatggctgag ttggaacata tggggtatat atacaaaaga
     2701 catggaaaag gaacttttgt tgctgcacta agtcaaaatt ctcaaaactt aatggaaagt
     2761 tatagtttta cagattatat gagaacacaa ggaaaaaagc caggtacaaa ggtattatca
     2821 tttgaggtag tagaaagtac aaagtatttc tctgaaaaat taggtataac tcctggtgaa
     2881 aaaatgataa aaataatgcg tttaagactt gctgatgacc ttcctatgat gcttgaaaga
     2941 acatatcttc ctatgaaaga atttatagga ttgacaaaag aaaaaatcga acaaaaacct
     3001 ttatatgaaa tatttagaga agattatggc gagattatta aagttgctga tgaagaattt
     3061 tcagctggaa taatctctga taaggaagct aagttattgg aagtaccagg agattcacct
     3121 tgtctgaaat tacttcgtac cacatataat gatgacaata tagttattga atttactttg
     3181 agtgttgcta gaagcgataa atttgtatat agagtaagac atgtaagata gtaagttaaa
     3241 taactttcaa gaaaatagaa tgatattata aacgtcaatg ccacaatgat agtataatta
     3301 taaggaggtt attttgaaaa gttttattta tgcagataaa ttttttatga aacatggaat
     3361 caaagaaaaa ggttacctta gtataattaa tggcaaattc ggtgaatttc aacaagaaag
     3421 acctaatgtt actgataatg ttatagattt ttcaggtaaa tatattgctc ctggcttagt
     3481 tgatactcac attcacggtc ttttaggtgc agatgtcatg gacaatacat ttgaagcaat
     3541 aaacacaatt tcaaaaggct tattaaaata tggagtaaca tctttcctac ctacaacttt
     3601 aactgattct gtagatacac taagtgattc tgtagaaaat ataagcaata gcttcaatcg
     3661 agttgagggt gcaaaaatac aaggtattta tttagaaggt ccatttttta cagaaaaata
     3721 taagggcgct caaaatgaaa aatattttaa agaccctgac atcaatatat taaaattatg
     3781 gcaaaaaaaa tcgaaaaatt taataagaaa aatagcaatt gctccagaac gtaaaggttc
     3841 aatagaatta acaagatatg caactaaaaa taatatatca gtttctcttg gtcatagttc
     3901 agctacattt gagcaagtta aacaagtcgt taattcaggt gctaaagtgt ttgtacatac
     3961 atataatggg atgagtcctt taaatcatag agaacctgga atggttgggg ctgctatggt
     4021 actaaaagat acttatgctg aattaatttg tgatggtcat catgtaagcc caatagctgt
     4081 taaaataatg atggatgcga agtctagtaa taacatagct ttaattacag attgtatgag
     4141 agctggtgct atggaggatg gaaaatatac tcttggtaag tttaatgtaa atgtaaaaaa
     4201 tagtgttgca agattaaata gtgggagttt agctggaagt gtattaacta tggacaaggc
     4261 tgtgagaaat atagttgatt ggaacatatc aactttagaa gatgcaataa aaatggctac
     4321 atatgtccct gctttaagtt gtaatattga taatgtctgt ggctctatat atcaaggtat
     4381 ggatgcagac tttatagtaa cagataatga ctttaatata cacgaaactt atataaatgg
     4441 tatctgtaag tacaaagtta ataaataggt tcaatataag gttatggagg ctgtctaaaa
     4501 agagtctgga aatatttaaa gttatttctc caaaatctga tttaacactt ttatttccat
     4561 atcgttaata aactattgcg atagaggtta ttttaaacaa atatatatta taagtatttt
     4621 tgtgataata ttttcgataa tatgtacatt taaagtaact tttctattat tatttgtaag
     4681 tttttatctt gagcaaacat ataaattttt aataaagcat gagataaacc ctatatttca
     4741 attactaatg atgagatttt gattgataac agatatattt tcaaatgtaa aaatactaaa
     4801 aatcatatca attttaaaga tattgtaggt gtaaataaac aagataatac tatagaaatt
     4861 tttacaaatg aaagcactta ttgtatatat ctaaaagttt taagcattga gagcgaagaa
     4921 tatctaatag atagactaaa atcattttgt tgattttata tattttatgt taaatataaa
     4981 gcagagtagt taaaactacc ctgcttttgt aattaaaatt taaaaataat aactcataat
     5041 acattgaatt tgagcttaac agtaactatt gtaataatag tatcaatctt ttgtgttgaa
     5101 tataatttat ttacactata gttaagcatg atttctaatg ttattttctc taatttacaa
     5161 ttatgattaa aattcattaa tatatatatt tataagttgt attgtttata ttgtttatta
     5221 cttttttaaa tccactagct gtaataataa atgtcttttc tggtatccct ggtgaaaaga
     5281 ttattttatc atggttaaat atatcttgat ctattacaag agagatattt ttctcatatc
     5341 caaaaggaga tacacatcca ggcaaacatt gtgttttttg tgttaattct tcttttgaac
     5401 atatagatac ttttccacct aaaacatttt ttatagtttt tggatctaat ttttttcctt
     5461 ctgtggttac aaatatatag taatttccag atctatcttt tataaataaa cttttacttt
     5521 ctactccagt atattgtaat ttttctctta tttctttagc tttttcaaat aataaaatag
     5581 gttcatggtc atattcttca aacgttatat gattttcttt taaaatattt ttgatattgt
     5641 tgtacatata ttttcctttc tatatttaaa ttaatttata tataatattc aattataaag
     5701 tgattttata atttcttcaa actatcataa aaaacattca catgttttag gaataaatta
     5761 tataaaatag gttataagat aatgaacatt tatattataa cctattttta tatcgataag
     5821 tatgaaattg tttaaccaag tcaaaatgat atataaattt atatttaaat gtgtttagag
     5881 tttaatttat tttcaatgtt taaaaaagtt attctatgca tctaatttaa ttagtaaact
     5941 gaggttctag ttagataaat tatattataa tgaatatata gtatgcagtt tacgatattt
     6001 taaaagtaaa tgatataata tttggaacaa ctgggttgca atgtgttttt aaaaaatatc
     6061 aaaataaggc tatgattttt aaaaccatag ccctgtctgt aaaaatttaa ttatcactta
     6121 aaaaataaat ttttatttaa agaatacaca taaatatttc cagactccct catgaactaa
     6181 aaagttcatt ttgagacatc tcttttataa tgttctttat tttatagtat aagtttataa
     6241 aaattaaatc ctatttatca atctgttctc taagccactt agttgctaca tctaactgtt
     6301 tattttcatc tttttcttca atagtaggct ttataccttt ttcattgata taatctccat
     6361 taggtgtaaa atattgagca gtagttaact tgtatcctgt tccatccttc aatcttacaa
     6421 ctgattgtac caacccttta ccaaaagttg tagtccctac agaaatacct tctttattat
     6481 caacgattgc agctgttaat atttcagcag cagaagcact ttctccatta gttaacacta
     6541 ctattggaag tcccaatttt tctttatcag atttcaagta ctcagtattt cccttattat
     6601 cttttgtata tactatagtt ccttcaccaa ttaactcatc agcaacttct ttacatatat
     6661 ctagcattcc accaggatta tttctaaggt ctattactaa tcccttgata ttttttgctt
     6721 ttaattcttt taaagcttta tcaaagtcag tatatgtact tgatataaat tgggtaattt
     6781 ctatatatcc taaatcatta tcaattactt tactttctat actcttctca attatctggt
     6841 ctgtttttac tttaaagttc aattgtttat cttctcttaa tatagtcagt tcaactgttt
     6901 tacctttttt acccttcatc atacgaacag cttcatctga atttttatat gagacagatt
     6961 ttccattgac ttttactaat ttatcccctg attttacacc tgctttctct gctggagaat
     7021 ctttcatggg aactactact aagccaccat cttttgatgc ccctatatac atacctacac
     7081 caacataaga accttcacta tcattaatta gattctccat ttcatcttta gtataataac
     7141 cagaatactt atcatttgtt cctagaaata gaccttttgc tgctccattt accaaatcag
     7201 tatctgatac ttttttataa aaatcttgtt ttataataga ttctagtcct aacaacttat
     7261 catatttttt atagtcttca tagagttctt tcgatattac aactttattc cccaatgtca
     7321 actgaaatgc tgatgtaacc atagctgtta taattactaa aatcacccct aaaaaaatag
     7381 ctttcttctt tgaaatcaca agatacatct ccttttacat aaactattat tattatatga
     7441 ctagctgtta tttttattac atttacccaa atatattgct ttattacaat aaattatact
     7501 aaaatttata ttaaatacaa ttgctgtaca cattataaca tattaaggga ggaattattt
     7561 tatgaaaaaa gaatttagaa aaaaagttat tgaatcaaga aaaaataaga gtgcaaactt
     7621 cgtacttagt aattcaaatt taatcactga aaaattactt caaatggaag atataagaaa
     7681 tgcaactaat ataatgcttt atctaaattt taacaatgaa gttcaaacag ataatttaat
     7741 caaaaaactt ttgtctctaa agaaaatagt atcaagtccc atcacaataa aagaaaatca
     7801 tacattaatc cctactcaaa taacagattt aaaagaggga ttgagaattg gagcatatgg
     7861 aataagagaa cctaacgaaa aatcacctac aattgatatt aaggccttag atgttgtaat
     7921 tgtaccagct gtagcatatg atatacattg ctatagatta ggatatggtg gaggatttta
     7981 tgatagattt ttagaaaact taagaaaaga tgctataact atcggcatag catttgactt
     8041 ccagatattc gattctattc caaaagaaac tcatgatgca caattagact acatagttac
     8101 tgaaactaga attatcactc caaataatta gaatattaag tatttccaga ccccatttaa
     8161 tatagtaata aatactactg ttaatatctt actttataag cattttaagt catggctttt
     8221 acaacaataa gaaccatata caatttactt acattaataa aaaccatgac tttattgtac
     8281 aatatgcagt tatttaatta taaaatacct tcttatttcg tcgcatttaa ttctattcta
     8341 tctggaagtt cactcatatt tgtttcaaca actatgtatg gaaaagaatc ttccttatct
     8401 ttatacttta tcttgactct caaaacatcc tttccttcct caaccacact ttctattttg
     8461 tccatttcta taccatattt attatttcct ctagtcacta taatgtatat tttgttatcg
     8521 attttagccg ctaatgctct ttcttcattt gtatacttat ccatcacatc taatatcttt
     8581 tctggtattg aatttctttg taatactgta taatctatgg gtttagattt ctctacaaac
     8641 atacttggta tgaatataaa tccccctata acgaatacaa gagctataac tccataaatt
     8701 atacctttaa tattaaactt tacattcaat cttatcactc ctcccatata ttctaaatat
     8761 atgatttata aacttatctt agactctaaa ttattgatta gtatatattt atacataatt
     8821 ataaatatat gtaatgtaac taaaaaacat gttttttcta tttcataata aacgagctaa
     8881 aaaataaaaa gagctatatg aaaacatttt cattgttttc atatagctct tgagtttaca
     8941 atattaaaaa atacttattt gtcactgatt ccttcttgat atccagatgt ttttgtaatc
     9001 tgtctataaa gaacacaaac cagtattatg acgcccatat atccagtata tggatataaa
     9061 ataccaacca gcttatcaaa tggtaggaag ccacctataa gacctaatat agctgctatt
     9121 aaagttatta atttaaactt aggatgttta tcatctacaa atctattagt tactgaccat
     9181 aataaaggta cagctgttgt atatatacct aatataagaa ttattgagaa taaaactcca
     9241 attactggtg aaatcttatc tgctaagaat aatgatggta ttgcttttga atataagttt
     9301 cctatatcag aaagaatagc taagtgcatc attatagctg cagccataag tgctactcca
     9361 cctaatatac caccacaaat agcttctttt ttgttagcag cacttgctcc taatccagtt
     9421 aagaatgcca taacaacaat tgtgttataa gtagtatata ttacaccaga tagataggca
     9481 tttggaactg gtttagctat ctttacagtg ctaagaactt gtgcagcttc tcctatatta
     9541 cctatgtttt tagctaaact tataccacca actaaaagtg taaagattat tattataggc
     9601 cctattccac ctagtatctt tgataattta tcaagaccta gtgaaacagt taataatgct
     9661 actactgcca tacctactct acctacaaat ggatttaatc catagtattc agttaaagtt
     9721 gctcctgctc ctgaaatcat tactacgaaa acaccaaata agaataaagg tccaaaccat
     9781 tcaaagaata ttcctaaata cttaccacaa taaacacgat aaattttgat aggttcttta
     9841 agttgaagct ctttaccttt tgacataact gaagcaccca tccatgagaa taataccatt
     9901 gaaattatcc caccaataat acctgcaaaa ccataaaaag agaagaattg cattatttcc
     9961 tgtcctgttg caaatccaga accgattact gttgctacat acgcacctgc gaaactgatg
    10021 actgttttaa catttacttt ttctgacata tctcagttac ctccccatgt gaaatttttt
    10081 cttctgatta aacaattcga taaactacct acaatttcct ttaacataaa atacatgata
    10141 actgttttat agctttttag tataataact taaaacttat aatctgattt tataccctac
    10201 tcctcctctc ataatttttt tagattcttt tatctaagta gtcattatat ataaaattag
    10261 tttctctata aattatatat tagtcatatt tttctgaata gtcaatatat atttgaatat
    10321 tttctctatt tttgtaaaaa tttgaatatt ttattacatt tttgatatat tgatatcata
    10381 taaaaaaaag agagatataa attaatataa cactttatac ctctctttta aatatattat
    10441 attattaaca aacatgaata atttatatct cttacattct ttttgcaata ataacttctt
    10501 taacagtctt tattatgtca tcacaagtta attcatattt ttttaacaat tcatttggag
    10561 taccagattc tccaaatgta tctttaattc caactttctt tacaatggtt ggacaagact
    10621 ctcctactac ctcacttaca gcagaaccta atcccccaat tatgctatgt tcttctacag
    10681 ttacaatagc attcgtttct tttgctgatt ctaatattaa atctttgtct ataggtttta
    10741 tcgaggacat gtttataact ctagcactta taccttcttc ttgcaacttt tcttgtgcta
    10801 atagagcttc atttaccata attccacaag ctataattga tacatccttt ccttctctta
    10861 agactgtacc ttttcctatt tcaaatttat aatcttcatc aaataaaact ggtactgaac
    10921 ttctccctaa tctaacataa acaggtccat tatacttagc tatttcaaaa ataatttttt
    10981 ctgtttcaac tccatcagct ggaacaagaa ctgtcatatt tggtatagct ctcattatag
    11041 caatatcttc cacactttca tgagatgctc catcctctcc aacagtaaga cctgcatgag
    11101 tagcacatat ttttacattt agctttggat aacaaactga atttcttata atctcaaaag
    11161 cccttcccgt agcaaacata gcaaaagtac ttgcaaaagg tattttacca gcagttgata
    11221 atccacaagc agcgcctatt aaattttgtt cagctatacc catattaaaa aatctatctg
    11281 gaaatgactt gtaaaaatca tgtgtttttg tagactttga taaatctgca tctaatacta
    11341 caacattgtc atttatttga cctaatttta ctaatgcttt accatatgct tctctcgtcg
    11401 ccattttact cattgtcagc acctcctaat tcaagcaaag ctttttctag ctcctcatca
    11461 cttggagctg tcccatgcca tcctgcttga ttttccataa aagatattcc tttgcctttt
    11521 atagtttttg caactatcat tgtaggtttt ttaactgttg atttagctat atcaagagcc
    11581 gcaaatattt ggtcaaaatc atgtccatct atctcaatta cattccaacc aaaagcttta
    11641 aatttatcaa ctattgaacc taaactcatt acgctttcta tatctccatc tatttgaagt
    11701 ccattattat ctaaaaaagc aattaaatta tcaagtttat aatgtgcagc actcatagca
    11761 gcttcccaaa caattccttc ttgaacctca ccatcaccaa gcaaagtata cacattccac
    11821 ggagcattat ccaactttga tgccatagcc ataccacatg caactgaaaa accttgacct
    11881 aaagaaccag tagatatttc cacacctggc gtgcctttca tgtctggatg accttgtaac
    11941 atccttccta tcttccttaa tccattcagt tcttccttat caaaatagcc tttttcagcc
    12001 aatgtagcat ataatgctgg tgcagcatgt ccttttgata atacaaatct atctctatcc
    12061 tccattttag gatttgaaga atccacattc atttcatcaa agtataacgc agtaagtatt
    12121 tcaactactg ataatgaccc tcctggatgt ccagatttgg ctctatgtat cattgaaact
    12181 atatctcttc ttatgatacg cgcaatctca tttaatcctt tatggtctct catgcaatta
    12241 cctcccttga gttcgtaatt ttatatcaat tccatattat tatatcactt ttatgctatt
    12301 tttttgcgtt atttttaatt ttattaacat agtacattaa aattaaacct taagagatat
    12361 ttaaaagaaa aaagggattg tctaataaaa gacaaatccc tctttaaaaa gtaaacaatc
    12421 ctaaacttat ttgaagtcca tcatctactt ttatcatcat attttcatct agacaaccaa
    12481 ttttttccct cagtctcttt ttatcgatag ttctaatttg ctctaaaaga attactgagt
    12541 ctttattaag cccatattca ttggaactta tctctacatg agtaggtaat ttggctttat
    12601 taatctgaga tgttatagct gatactataa ctgtaggact atatttatta cctacatcat
    12661 tttgtattat cagaacaggt ctaacgcctc cctgctctga accaacaact ggacttaaat
    12721 cagcgtaata taaatcacca cgctttattt cacaattagt actcaccttg ggcaatcttc
    12781 ccttcatttt tatcatcaga agatacttct tcaactgtca tacctaattc agctagtgac
    12841 agattaattt gacccatttc tctataaccc gttttcatag attctttaat atgaattttc
    12901 tttttctgag ttatatagaa ctgaaaagca gcttttgcaa aatcttctct gttactgttc
    12961 tccatctgaa ctatactatc aacttcagaa atcagagagt caggtaaagt aacttctatt
    13021 ttttctttac tcttatcgta cacaaaatcg cctcccagat tataatatca tggttttatt
    13081 ttagcaaata actgtttatt tgtacaagct cattattttc catataaaca cggtcaactc
    13141 ttcttgatat catacataac acttcatagt ttatagttcc taagtcttta gctatacgct
    13201 cagctgtaac ttcgccttct ccaaatagta taacctcatc tccaactttt atgtctatat
    13261 ctttgtcaat tctaaccatt atttgatcca tacatattct accaactaca tcaaacactt
    13321 ctcccttaat aagaaccttt ggattttttt ggattctagt aaatccatct gcgtatccta
    13381 ttggaactgt agctattgtt tctttacctg tagttgtgta ttttagtcca taacttattc
    13441 ctacacctgg ttcaacttgt tttatatgtc cgatttttga ttttaatttc atggctggtc
    13501 ttaattctaa tctatcttta aatacatcat cagatggata atgaccatat aatattattc
    13561 ctgctcttac catatttaat cttaaatcag ggcaatccat aatagctgca ctgtttgata
    13621 catgttttat agctattttt acaccagcct catctaattt atcagacata aatttataat
    13681 tattagcttg tttataagtg tactctttac ttacttcatc agctgtagca aaatgagtaa
    13741 acataccttc taaatctata tattctaatt tatttaattc tattatttcc tgtactgact
    13801 tctcattagg ttggaaacct attctagtca tccctgagtc tattttaaca tgaacacagg
    13861 ctttttctcc taaagatttt gcaatttcat ttatcttttg tgctgtttct aaagaataaa
    13921 ctgtcatagt tattttattt tttatagaat cttcaaaagc ttcgtctgga gtatatccca
    13981 agttcaaaat aggaagtgtt atgccatttt gtctaagttc aattccttca gcagttcttg
    14041 ctactgctaa gtaatctact ttttcttttt ctagcaattt tgcaacttct actgcaccat
    14101 gtccatatgc atcagctttt attactccac aaatcttaat atcttcttct aataaatttt
    14161 taatattatt taagttaaat cttaagttat ctagatttat ctctgcccat gtaggcactg
    14221 ttattttttg catgtcttta gtccccctta gtttctgtat tcaaaatctt tgtattttac
    14281 tgtaaatctt ggcactccct ctttatctaa cacttccatt ttttcaggat tttttgtgtc
    14341 agcatttaca tataatactt gcttgttaaa atatttatta tccccaggaa taaaagtctc
    14401 taaaaccaaa tgtcctttgg ataactttac tttcatctcc tcattttgaa gatagttttt
    14461 tataaagtct cctacaaaaa gatattgatt attttttcct gtattaggta actccactac
    14521 atcactaatc tttggatttt taactaatat tttatcatct tgatattcta tacttttccc
    14581 ttttaaatgt tttggggaaa ttacttctaa tttatagtta tcggtttttt tataagtatg
    14641 aattaaaaca taattgtgtg ggcttttatt tccaactact tctacttcag ctttacaact
    14701 gtaataattc atatcagaaa tctgcttttg aaaatctttg tatacttctt cttttgtgga
    14761 ctgtcgtttt tggcatccta ttacaattat aaccagtgcc aagaacataa tacatactat
    14821 ggtccacttt tttctcacat cacacctcct acttcagttt attcattgta taaagctttt
    14881 attatatcat atctacaaat tataccttta agtttgttgt catcatctac tacaggaact
    14941 ctgtttattg attttttaat cattacatta gctacatcat caaacttatc atcttcatga
    15001 acctttatta tatctttaga cataagttct ccaaccttat aagcagctac ttgttttata
    15061 tctttttcaa ctttttttaa gtcatctaag aaaatcaatc cttgtaatag atttatatag
    15121 agaggtgctt ctatatattt ttctttttta agaatatctg tttcagaaat tattccaaca
    15181 actctattct ctgaatctac aactggcaga cctcctattt tttctgcaat tagcatatta
    15241 gctacatctg ctatactatc atcttgtttt gctactatta catctgttgt cattatttct
    15301 tttgctgttt tatccatcat aatgtaatca ctccttagtc cttaattatg gttatcgcat
    15361 ttgctatagc ataatctttt gaatgtgata ttgaaacttt tatttctaaa acattataat
    15421 ctatgcacat ttttgctaga ttattataag tttttactat tggttttccc atttcatttc
    15481 tgagaacttc tatatcttta aaattaaaca atctaattcc tgtacctata gatttactta
    15541 tagcttcctt ggctgcgaaa ttacctgcta tactttctgc tttaaaattc ttagaattaa
    15601 aatactctat ttctctatct gtaaatatct tctccaaaaa cctattattt ttgtctacag
    15661 cctttcttat tctatcaatt tcaattatat ctacgccaat atcaaatata ttcattgttc
    15721 ctccagaaaa tttataatac ttctttcaat aattaataat gaatttataa atttactatc
    15781 gataatacac attattattt agtttatgtt acttatctta atttaataat cattataata
    15841 aaactgtaac tttt
//
LOCUS       DAYATN010000087        15838 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.087, whole genome shotgun sequence.
ACCESSION   DAYATN010000087 DAYATN010000000
VERSION     DAYATN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.087"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..434
                     /locus_tag="ACGI8A_002514"
     CDS             <1..434
                     /locus_tag="ACGI8A_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFH0180506.1"
                     /translation="RNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQV
                     KNMIRNRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEE
                     VKDLNIREWIYPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            866..1120
                     /locus_tag="ACGI8A_002515"
     CDS             866..1120
                     /locus_tag="ACGI8A_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180507.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1156..1479
                     /locus_tag="ACGI8A_002516"
     CDS             1156..1479
                     /locus_tag="ACGI8A_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HFH0180508.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1753..1905
                     /locus_tag="ACGI8A_002517"
     CDS             1753..1905
                     /locus_tag="ACGI8A_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180509.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2174..3022
                     /locus_tag="ACGI8A_002518"
     CDS             2174..3022
                     /locus_tag="ACGI8A_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFH0180510.1"
                     /translation="MWILVPIITIVLLIIAVSSMQYILVMIAFLLIIYSFIEKKIVMG
                     LVSVLFFTYSIYLCATWEDKSLIADNKVETVRAQRETVEREKEMERRRIQEEVDKERY
                     IEKHGMEISENDLKVKLEALVPQEYKGKKYELKVGKFKRYSMYFDLTVQNEKFSNSEE
                     CKKFVKEIANDLKKIKISKAYFKFHSKDDGGIYNSVYIDYFRNIQNNVDNVENLEFNE
                     FELKTEEEEKREQEKIEQEKNSYNNYIQNRVVDPLDRIKKLKELLDSGAITQEEYNKK
                     KKELLE"
     repeat_region   3289..3642
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3289..3317
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            3797..3985
                     /gene="srl"
                     /locus_tag="ACGI8A_002519"
     CDS             3797..3985
                     /gene="srl"
                     /locus_tag="ACGI8A_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Srl"
                     /protein_id="HFH0180511.1"
                     /translation="MNKIATKSRAEYMKNCRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            4105..4470
                     /locus_tag="ACGI8A_002520"
     CDS             4105..4470
                     /locus_tag="ACGI8A_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFH0180512.1"
                     /translation="MKNELMMFEGKEIEVFEFEGRILFNSKDVANCLDIKNVNENIIL
                     MNEKQVVKLRNSDISNTDIRKLNNAGENFLTESGVYKLIFKSRKEEAERFQDWISDEV
                     LPSIRQTGAYITNNVIPKS"
     gene            4523..4918
                     /locus_tag="ACGI8A_002521"
     CDS             4523..4918
                     /locus_tag="ACGI8A_002521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180513.1"
                     /translation="MLKELLDDAGFDNKSKLLTAKTLYKKAEIDLPIEINEEEHYFDT
                     KQIASKLKIYSKSNKPAQMAVCEIIKKIDLEDGEVKGVWEINGSWTGTVNKYTKSVID
                     KVRTWIEENNRPTKIAGEKKNYYVFYKIE"
     gene            4970..5131
                     /locus_tag="ACGI8A_002522"
     CDS             4970..5131
                     /locus_tag="ACGI8A_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180514.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5459..5923)
                     /locus_tag="ACGI8A_002523"
     CDS             complement(5459..5923)
                     /locus_tag="ACGI8A_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180515.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
     gene            complement(6059..6376)
                     /locus_tag="ACGI8A_002524"
     CDS             complement(6059..6376)
                     /locus_tag="ACGI8A_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180516.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(6659..6955)
                     /locus_tag="ACGI8A_002525"
     CDS             complement(6659..6955)
                     /locus_tag="ACGI8A_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180517.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKNYLNRF"
     gene            complement(6974..7387)
                     /locus_tag="ACGI8A_002526"
     CDS             complement(6974..7387)
                     /locus_tag="ACGI8A_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180518.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7371..7604)
                     /locus_tag="ACGI8A_002527"
     CDS             complement(7371..7604)
                     /locus_tag="ACGI8A_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0180519.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            7823..8197
                     /locus_tag="ACGI8A_002528"
     CDS             7823..8197
                     /locus_tag="ACGI8A_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0180520.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
     gene            8809..9501
                     /locus_tag="ACGI8A_002529"
     CDS             8809..9501
                     /locus_tag="ACGI8A_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0180521.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            9697..11757
                     /locus_tag="ACGI8A_002530"
     CDS             9697..11757
                     /locus_tag="ACGI8A_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFH0180522.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(11962..12285)
                     /locus_tag="ACGI8A_002531"
     CDS             complement(11962..12285)
                     /locus_tag="ACGI8A_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFH0180523.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(12278..12979)
                     /locus_tag="ACGI8A_002532"
     CDS             complement(12278..12979)
                     /locus_tag="ACGI8A_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFH0180524.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(13257..14642)
                     /locus_tag="ACGI8A_002533"
     CDS             complement(13257..14642)
                     /locus_tag="ACGI8A_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFH0180525.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(14750..>15838)
                     /locus_tag="ACGI8A_002534"
     CDS             complement(14750..>15838)
                     /locus_tag="ACGI8A_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFH0180526.1"
                     /translation="SKINLNILNINNTTINIYKHNIDNYIPYDQGITLDIFMYIWILG
                     IIAISFYYLRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYS
                     PAIIGLTRCKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPIL
                     YLLKKYFHELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKK
                     NIILKKRLDSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYN
                     KNGETYGTHIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTR
                     KRNFNIFKKTIPLYDKEGIKIIGRFEIN"
BASE COUNT         6254 a         1723 c         2390 g         5471 t
ORIGIN      
        1 atagaaataa agcaagatta aaagttgcaa gacttcaaga acatatagct aatcaaagaa
       61 atgattttct acaaaaacta tctactaaat taataaaaga gaacgatatt atttgcatag
      121 aagatttaca agtaaagaat atgattagga atcgtaaact atctcgctca atttctgatg
      181 tgtcatggtc agagtttatt cgtcaattag aatacaaggc taattggcat ggtagacaaa
      241 ttgtaaaggt gggtaaattc tttgcaagtt cacaaatatg caataaatgt gggtataaaa
      301 acgaggaagt gaaggactta aatataagag aatggattta tcctagttgt aatgaaactc
      361 acgatagaga tataaatgca agtataaata tattaaaaga aggattaaga ctaataacaa
      421 ttcaaaataa ataacgtatg taagaaccgt aggaactacg gggatagcct gtggagaatt
      481 agtaagacat atgttagtat gcaaaattct gtgaagcagg aaacctgtga ctttagtcat
      541 gggaggttca ggctagtatt actcattatt gtattcaaaa gctaaagtgg aaacctagtg
      601 aatatatgaa tttagaagtt aatgagagag cattagcagc cgactcaata cttattaaaa
      661 ttgaagatga agaagaaaca atgaaagaag ctgaaagaga gaaaaagaga ggtagaagaa
      721 ggtagaattt aaagttaaaa tagttacatt gctaatatat gaaaatgtta caaatttgga
      781 aatagatgga tatatatgtt gtatagtata atgtagataa tatatcacca agtaaaaaac
      841 aacaaataaa tctaaattat gaggggtgga aaataaagat attaaactta caatatgtat
      901 atttgctttt gtaattggaa taatagtgtt attttgctgt ataggtctag gtcaatttaa
      961 tatggattgt attataaaac aaaatggtgg gtcaatagct accgaacaat acaacatata
     1021 tttagaacaa tctatttcac aatatagaaa ttttggttct atactggttt tactaggagg
     1081 aataggaata ctgtttgata aaagtgtcag aaaatactaa aaaagtatat ttttatttag
     1141 gatgtgatga atgatatggc aagagatgta tttagtgtaa agatgacacc actagaagca
     1201 cagaaaatgg tagaaaaaaa tttggaagca gatttagttt ctgttgatat ttataaccta
     1261 gaaaataata agtctataat agttacaatc tttgaaaaat attatgcaag aagcaatagt
     1321 gatgcaggtt tggtagtgat ttgtgaaaat actacaggaa aaacacttgt taaaattacc
     1381 tcaactggta gtgcaattgg actatttcag attgattggg gaacaggtaa taatttgatt
     1441 aaaagagtaa aaaaaatact tattgattat attatttaat tattttatgt agatattctt
     1501 aggttggtat ggaatttgta ctaacctatt aagtaatcaa taaaactaaa taaacataga
     1561 aagcacttac aaatatgtag gtgcttttta ttgaaaaaat agaaaataaa ataaaaagta
     1621 agatatagga aaaatatgta agaattatat gttataataa ttgtagcaag aagatgtaat
     1681 ctacaattta tagagtggag ttcatgtatt aaaaaattat cctcccaacg ataagaaggg
     1741 aggtgaatat acatggataa ttttttattt aatgtgttag ctagtgtaac agctagttac
     1801 atagtttatt tgattagcaa gttaatcaaa aaagtaaaaa gccactctac cgcaaagagt
     1861 gacttaaaga ttgatataaa ttttaaatat catcgtaaaa aataactttc agttatagaa
     1921 cttcactcta ccgcaaatag attgtagttc ttcttgtttt tattatacca caaattagaa
     1981 aaaatattgc atataatatt ttttatagtc aataaaaata tgaaattttt tatacacaat
     2041 gatgatgaat ctaactagat gaataaattt aattgtaaat aagaaagtac ttacttaaat
     2101 ggtaggtgct tttgttttgc caaattatgc tataatattg agtgagatga gtaatttgtg
     2161 ggggaatttt ataatgtgga tattagtacc aataataaca atagtattac ttataatagc
     2221 tgtttcttct atgcaatata ttttagttat gatagctttt ttgttgatta tatattcttt
     2281 tatagaaaag aaaatagtta tgggtcttgt gtcagttctt tttttcactt attcaattta
     2341 tctttgtgca acttgggaag ataaatcctt gatagcagat aataaagttg agactgttag
     2401 ggcacaaaga gaaactgtag aaagagaaaa agaaatggaa agaagaagaa tacaagaaga
     2461 agtagacaaa gaaagatata ttgaaaaaca tggaatggaa atatcagaaa atgatttaaa
     2521 agttaagcta gaggctttag tacctcaaga atataagggg aagaaatatg aattaaaagt
     2581 aggaaaattt aaaagatata gtatgtattt tgatttaact gtacaaaatg aaaaatttag
     2641 taattctgaa gaatgtaaaa aatttgtaaa agaaattgct aatgatctta aaaaaattaa
     2701 aataagcaag gcgtacttta agtttcattc aaaagatgac ggcggtatat ataattctgt
     2761 ttatattgat tattttagaa acatacaaaa taatgtagat aatgttgaaa atttagaatt
     2821 caatgagttt gaacttaaaa ccgaagaaga agagaagcga gaacaagaga aaatagaaca
     2881 agaaaaaaac agttataata actacattca aaacagagtg gttgacccac tagatagaat
     2941 aaagaaacta aaagagttgc tagattcagg agcaattaca caggaagaat ataataaaaa
     3001 gaaaaaagaa ttattagaat agataatata gtaagcactt acaagtatgt aggtgctttt
     3061 aaatttacaa agtattccat tttaatttta tagtttagat tttatgatat aataaaaata
     3121 tagaagtttt gcagtgtgcg atatttgtta caaagtaggg cttaatactt gaaatctaag
     3181 atgttgaggg tgcgtgataa gtgttatcaa ttgcactatt gcccgctcac tgcaatttta
     3241 agagtattgt atatatgtag gtattggaaa tgctaagttt attttggggt tttagattaa
     3301 ctatatggaa tgtaaatatt acatgggtaa aaattttgat gatttttatg acagttttag
     3361 attaactata tggaatgtaa attgtataga attaagaatt tttttaatac taattatagt
     3421 tttagattaa ctatatggaa tgtaaatgat gaacaataaa acaatcatct aaagacgaag
     3481 aaagttttag attaactata tggaatgtaa atgttcctgt tgtgtttttt actaatccca
     3541 ttttatatgt tttagattaa ctatatggaa tgtaaattgc tgtgatttca ctaataaaac
     3601 aattaataat tgtgttttag attaactatg tggattcaaa attaaataaa caaagaaagc
     3661 acttacaaat atgtaggtgc ttttattctg ctcaaaattg gtcggttggg taaaatcatt
     3721 agaaaaaatt agtaaaaacc tcttttctgt aactcgttac aatattatta ttaatgtaac
     3781 gagttacaga aaagaggtga ataaaatagc aactaaaagt agggcagagt atatgaaaaa
     3841 ttgtcgaaaa gataaaagag gttttagtgt acttttagac aaagaaaagt tagataaatt
     3901 tgatgaagta ttagaggaaa agaatctaac caagaaagaa tggctagaag aaaaaatcga
     3961 cgaggaactg gaacaaaagg aataaaaaat aagggtcact cccaccgacc aaagtttgag
     4021 taacccctat gatgtatact atcgtatatc aattataata tatgtcattc cttaaaaaaa
     4081 tcaattatta aggagtgtaa tattatgaaa aatgaattaa tgatgtttga aggaaaagag
     4141 attgaagtat ttgaatttga aggaagaatt ttattcaact caaaagatgt agcaaattgt
     4201 ttagacataa aaaatgttaa tgagaatata atacttatga atgaaaagca agttgtaaag
     4261 ttaagaaatt cggatatcag taatactgac atccgaaaac ttaataatgc tggagaaaat
     4321 tttcttactg aaagtggagt gtataaatta atctttaaat caagaaaaga agaagctgag
     4381 agattccaag actggataag cgatgaagtt ttaccaagca taagacaaac tggtgcatac
     4441 ataactaata atgtaatccc gaaaagttga gagaaaaagc aagtgagatt gaaaaattac
     4501 agttgcttat aacagtacat ctatgttaaa agaattgcta gatgatgcag gctttgacaa
     4561 taaatcaaaa ctattaacag ctaaaacatt atataagaaa gcagaaattg atttaccaat
     4621 agagataaac gaagaagaac attattttga tacaaaacaa atagcatcta aactgaaaat
     4681 atattctaag agtaataaac cagctcagat ggctgtttgt gagattatta aaaagattga
     4741 tttagaagat ggtgaagtta aaggtgtttg ggaaattaat ggttcttgga ctggtactgt
     4801 aaataaatat acaaagagtg taatagataa ggtgagaact tggatagagg aaaataatag
     4861 acctaccaag attgcaggtg agaagaagaa ttattatgta ttttacaaaa ttgagtaaat
     4921 ttatcagttg tattaaataa tttagtttag ttttgggggg attaatacaa tgtgtgagaa
     4981 tttacttgat aatatgtata ttgaaaagag aaaagaagaa tatagaatta aacttttgaa
     5041 aataagagaa acagatatag atatatataa taagttagaa agtatagtat ataaactttc
     5101 tgagaaaaaa ttagagaaaa ataataaata gacatataaa gcacttggat attctactgt
     5161 ttcaagtgct tgtttggtat aaaatggtat aatggagata gagaattata ttaaccaaaa
     5221 aatggagaaa atagagaaga tgatttagtt gacgctttat cagtaggagt acctgcaggt
     5281 aagacagaat ctctagtacg attagttgga gatggaataa gtgatactca aaaaagttta
     5341 ataaataaaa gagaatttag aaatataact caaattggtg gaaatggaaa tgagaaagca
     5401 tttagtgaag tagaaaactt agttaaatag attagaaggt agcaactaga gttggttgtt
     5461 accttctttt tttatacttt ctctctctaa gtattttatt tttcatatct tctaatacat
     5521 ctaatgcagt agtataaaat tgtatattat ttctataagg tatagctatt ggaagaatgc
     5581 ccttgttaac taaaaaaata agaagtacaa taaataaaag tgtcataatc attctaataa
     5641 aataattagt gatatctgat aaatcaaatg ttaagttaat tgttgtagaa aatacaacag
     5701 ccataataga tatatacaaa gcaaaaatgg ggttactatt tttttgtaac tgtcctttaa
     5761 gcctagattg ctctatttct atatcttgaa tactttttat ggaatccatt tcactataat
     5821 acttataaac ttttaaataa tattttttat acttgtttaa agcttcttca ttatctttaa
     5881 ggtctttgtc acttaactct gatttaactt ccccaaaatc cataaaatcc ctcctacaat
     5941 gttatataat atactttaat ttatagcata tttaagaact tatatcagca atttgtcgaa
     6001 cgattttagc taaaaataga gtataatgat aggcttatta tttataagat attatttttt
     6061 atatagttat atatttcttt ctaattagat aatataatgc tgttgttata atggctacta
     6121 atattattgc tattattgta atatctaatc ttcttgtaaa taagcctatt gctatatact
     6181 caagacctgt tacatagcga taaagtactt ctagtttaac agtttttttc ttatctatat
     6241 ttccatatat tgattctaat ttattaaaat attttttact acaaaaagca gttttaggtt
     6301 taattaaata tattattgta gttcctatta taactattat tccaataagt ataagattga
     6361 tatttccaaa atccatagca gatacctcct attaaatttg cttttcttaa tcaaagtata
     6421 tcagtaacac aagtacaaag agtatctaaa atattacaaa tattaacagg atagataaaa
     6481 ctatagtaaa aattgtaata ataaagcgaa cgaaaattat catactaaaa cataagacat
     6541 gttataattg tattagatag aagattaaca tacaccaaag cactccttat aataaagagt
     6601 gcttattttt ttgaaattca tcaatatata aactatcaag aaccttattc aacacacctt
     6661 aaaatcgatt taaatagttc tttttgataa cataatttat atgatataat aaaaaaatag
     6721 aagtgtacca ccaatacact tctatagcta taactaagca ttctcttgct gggggagtgc
     6781 ttttttcatt tctccaaaca acattattta cttcatcagt aattatcaaa tcaatctcat
     6841 tatctttcaa aatctctgtt gcagtacaaa ttagattatc taagtcaaaa cttcgagtta
     6901 tctctagttg tttgttattt acttctcttt ttagtatata agttttaaaa tacatttata
     6961 tcaccccttg tatttactct tttttctcta aaaatatatt gataagtaaa tatgcaacaa
     7021 ataaaaaaat ctcaatgaca catagtgtta tagctataac tagaagtgat tgatttttcc
     7081 atgcactata agctatgatg aaaagaaatg taaaagtaaa taatgtataa ttcataattt
     7141 taactaagat atgacttgtt ttcattgtta agtacaagtc tctttcatct gtactatcgt
     7201 gtaattcttc aacaatgcct tttcttgaaa aagcattaca aaaattaaag attgctatta
     7261 caattaaaag aaatgatgaa attaggaatc ttttatcttt taaaatgcaa taaataacta
     7321 aactagcaca acataacaca caaactataa tacccactat aaaacttcgt ttactcttta
     7381 ctttcatgtt ttttatcctc catttcttta ttttctttta aacaacatag ttcttcaatc
     7441 gttgtaccaa acacttgtgc catacgatag gcaagcataa gagaagggct atattgctct
     7501 ttttcaatag aaataattgt tctgtttgat acatggacta aatcagcgag ttgttgctgt
     7561 gttagaccca tatttgttcg aaattctcgt acttttgttt tcattccata acctctttct
     7621 atatgaagct aacttcatgt gaagttaact tcataatatc ctaaaatgag taaaatgtca
     7681 atctcattag ataagtatta taaaacctaa aagaataata tggtaaaaat tggatggaat
     7741 tatttacctt aattctcttg tttgtaaaac tttattgata tagaatttaa catatacata
     7801 aatcggcaaa ggggcgcgaa ttatgaaaat tagtaaatta ccagaagcgg aattaaaagt
     7861 tatgagatat atttgtgaag ctaacaaggt attaacttct agggaattag ttgctgcaat
     7921 ggaagaaaaa tatgagtgga atgaatcaac tacatttaca gttttgaaaa gattagagcg
     7981 aagagagttt ttaagtacag agaaaatagg caaacttaca cattataata tactagtaaa
     8041 agagaaaaaa tatctaaggt ttgaaacaaa ggaatttttg aagaacatac ataaaaattc
     8101 aatttcaagt ttaatgtcag cgttacatgg tgaagacgac gaagtggatg aagataaatt
     8161 aagtgaactc gaagaatgtt ttaagaattt gaaataagaa aatgacaaat tgatacataa
     8221 taaataaaat gaaggtatga aattataact ttttctacct tcattttgtt tattattctg
     8281 aacatgttaa gaaaatgaaa taagtttgta ttttagttat ataaatttta atagaatatg
     8341 tacagtatat agtagataga atgtataaaa atagaattta aataagtagc tattattgca
     8401 gtgaaatgaa tgtttctaag tatatttggg tggaagaatg gtggaagaaa attagtgaaa
     8461 tgtaattctt ccaccattct tccacctaga aaatagaaac tgtttaataa gaattaatat
     8521 acattaattc agctgaatgc aatcaaaata cttaaatgaa agtaatctga aaacaacata
     8581 attcaaatca atttattcaa ataaacctca atttatctta aatcttaaga aatatttaag
     8641 aattaggata atattttctt aagatttata ttttattata tagttataag ggaaagttac
     8701 aaaaatgtaa gccaaagtaa tataacaatt tagtcattgt tgagaaaggg agtgaaagat
     8761 attgtataat atagataagt attattatac aatagaggaa gtgaaaatat gtacaatata
     8821 ttagtagtag acgatgataa agaaattgtt gattcgatag aaatatatct taaatcagaa
     8881 ggatttaaaa tatataaagc atatgatgga atgaaagctt tggaaataat aacagagaat
     8941 gacattcatc taatactaat ggatataatg atgcctaaat tagatggaat caaagcaact
     9001 gtaaaaatac gagaagaaag aaatattcca ataattttaa tttcagcaaa aagtgaagat
     9061 acagataaaa taataggatt aaatattgga gctgatgatt atataacaaa gccatttaat
     9121 ctattagagc ttatagctag ggttaaatca aacttaagac gatatgtaac tttaggtaat
     9181 tatgaagata catgtaaaga aattctaaaa agtggagctt tggagttaaa tacactaaca
     9241 aaagaagtta aaattgatgg tgaaaacata aaaatgactc caattgagta taaaataatt
     9301 gaacttcttt tagaaaataa aggacgagtt ttctcaatag atgagatata cgaaaaagta
     9361 tggaatgagg aaagttttaa ttcagaaaat acagtagcag tacatatcag acgaataaga
     9421 gaaaagattg aaataaatcc aaaagaacca agatttttaa aggtggtgtg gggagttggt
     9481 tataagattg aaaaatttta gtggaaaaat tataaaagaa aagagtttaa tatttaattg
     9541 cattttgatt ttagtcattt ttacatttac acaaatgtta ggaattttaa tatgttttaa
     9601 ttttttctct gagagtttaa tttactctat ggggattatg cttttcttaa actttatttt
     9661 atataaaata ctgacaatgt agatgaggtg ggcaacttgg agaaatatag agtattaaag
     9721 ttagtttcat taatcataat atctatctca tttccattaa ctatattgtt ggtgacaata
     9781 ggacttgctg agcctaaata caatatacaa aaaacttcta caaagagtgg gatttttgat
     9841 atatctgatg atttttatga tagtaatata tttgacgaag gaacaatggc aagtctatta
     9901 cccaagttta agctatctgt aggtggtaaa ttaactagta aagaaaaagg taaattccaa
     9961 caagaaggta aaagtataga tttaagtagt agtgattctg agtatgataa aatcatgatt
    10021 aatgaacaag cttctgaaga atatttaagt tatctaaaaa atataaaagt gtatgttatt
    10081 aatacataca caggagtatt ttatacaaat acgtcatatg ctagtttata tgattttaaa
    10141 caaaaaacta agagctattg taatgtagag ataataaata agataggaaa gagctcatat
    10201 attaaaacaa taaatggaga aaaatttgaa ctgaataatc tgagtctgga actaaatgaa
    10261 gtagatgaag gttttgaggc atatgtatct tttccagaag agccaactat agaagatgga
    10321 atagtataca ctaattttca aatatttaaa caagctacag aaaaagtaag attatttttt
    10381 gtagctagtt taagtttagc aattatatgt ttattatcta taattttata tatcaaaata
    10441 aaaggtgaag ttttaaaaag aaataatgta tttttactaa tatacagtaa gatacctatg
    10501 gaatttaatg taatattagt atctatttca attgcaattt taactatata tgctcttgct
    10561 gtatcttatc atattgatgt aattgcatta gtttctttat ctttactgat agtatcaaat
    10621 aatatatttt ttataaatgt gcaattgagc tatttagaaa ataaaatgtg tatatttaag
    10681 acaagtatta tagtaagagt gattttatgg ttaataaaaa ctgtaaggga ggtcagtaac
    10741 gctataaaga aagtatcttt agcaaggaaa gttattgtta tagtgataat atctttagct
    10801 ttaatcatgg caattagcac tcgaagtata ggtatgggat tactgttttc tatatgtatt
    10861 tttttaggat ttacaatata tataactaaa agactttcat atcttagtta cgtaatagaa
    10921 ggtacagaaa gaataaaaaa gggagaatta gattataaga taaaaattac tgggaatgat
    10981 aattttacgt ctttagctga aaatataaat aatattggag aaggtcttga tagagctata
    11041 gaagagcaag taaaaagtga aagaatgaag tctgacctta taacaaatgt atctcatgat
    11101 ttgaaaactc cacttacttc tataataaat tatgtagatt taattaaaaa agaagaatca
    11161 atacaaccag agtatataaa tgattatata aatgtacttg agtcaaagtc aaaaagacta
    11221 aaattattga tagaagattt atttgaagcg agtagagtaa gtagtggcaa tatagaatta
    11281 caaattagca agattgattt agtgcaacta ttaagacaaa gtataggtga acttgaagaa
    11341 aaattatcta aaaataattt atatttaaaa ttaaatgttc caaatgataa ggtgtatata
    11401 tgggcagatg gaagaagaat gtatagagta tttgaaaatc ttttatctaa tatagcaaaa
    11461 tattccttag aagaaactag agtctatatt gatgtatatg actatggtga aaatgtaaag
    11521 gtaactatga aaaatatatc ttcttatgaa ttaaattttg acccaagtga gattatggaa
    11581 agatttaaaa gagctgatga atcaaggaat actgaaggaa gtggattagg gcttgctatt
    11641 gcaagagatt tagtagcttt acaaggtgga aaattttata tagaaataga tggagattta
    11701 tttaaagcaa atttagagtt tcaaaaatat gaagaagaaa taacaaatat ggaatgagat
    11761 agtatttaag tatatatatt tttttactgt cgaaagcttt tattataata tattagaata
    11821 tttacagtag agaatgaaga gtaaaaacag agtaacttta agtcactctg ttttttattt
    11881 aaaatcaata atataacaac ttaaattaaa attgtacaca tagttttaaa ataacttata
    11941 caataaaaca ttatccaaat atcacaagat aattccaaat aaatatatag atatcactcc
    12001 tgtaataata gaaataccaa tagatttaaa cttatatgca acaagaagta caatcataaa
    12061 agatataatt ttagggttag ctatagataa atatagacta tctccattaa aaaatacatc
    12121 tttagcagtt aaggaagtaa gtattgctac aggtataaat ttcatgattt ttttcaaaaa
    12181 atcaggcatc tctttttttg aaaaaattaa cataggaagc actcttggta gatatgtaac
    12241 aatgtacatt ccaattataa taaaaaatat ataattatta ttcataagat tcctcctttt
    12301 tacagtaaga ttcttctata aagaaaccaa ttaaagatgc taaaattgat gcaactatta
    12361 ttactaaatt gcttttgaat aatgcatata aaataacaga aataatgatt gatgatataa
    12421 atactacaat ataaatctta ttttttattt gagaaactat taaagctata aacatagaag
    12481 tcaatatgaa gccagaaata gaactgtcta tagatatact ttctcctaaa aatgctccaa
    12541 taaaattagc taaaactgat gaaattaagc agaagaaatt aagataaata gcatttctat
    12601 cagaccaatt tccagcagta aatttttcat aattaactgc aaaagtttca tctgttatag
    12661 tatgacaaaa tggcaatata aattttggac ttttattttt tatgtacatg ttcaaattag
    12721 aactcattag aaaatgtctt aagttaacta taaatgtagt caaaacaata gatgttacac
    12781 ttgcaccagt agaaatcatt gaagctgcaa tgaattgaga acttcctcca aatactaaag
    12841 ttaccattag tgctatttga aaaggtgaaa gaccagattt ttgtaatagt attcctccta
    12901 ctatacctat aggtatataa cctaagaata taggaattga agcaataaag ctttctttta
    12961 attgttgagt tctttccaca ataaatcact ccttttgtat taaatgtatt aaagtattcg
    13021 taaaacagaa aaatagatta attgtataat tgcaattata attaaaaata aatttggatt
    13081 aatatataaa atactataca acaaatagat aatcttaaac ggttattaaa ttttatctat
    13141 ttgttattat gatgtaataa tacatattaa tctctataag ttaattacta tctggtgtaa
    13201 ttttatttta acaacaaaaa aaaatacaca caattaaaat attgtgtgca atacaattat
    13261 tttttctctt ctaatagtct ttttaatacg ctcaaaccag ctttaagttc ttctgtactt
    13321 tctgtagcac atacagctat tcttattgca gaaattggtt tttcttttcc aacagcaaaa
    13381 cgttcagaag catagacctg tacaccttct ttaagtgctt gtatctcaaa tttttctgcc
    13441 attattccat caggtagaat caaccatcta aatatacatt cttcatttcc caaaagatta
    13501 tatccagata aatattgatt aataatcttg tttcttcttc ttgcagattt acggcgtgca
    13561 ttaattaatt tatcggcttc acctgatgta atcatgcgat aagcaatttc tgttaaaaaa
    13621 taagattgag atagattaat gttgtataat gcgcttgata atgcctctcg atactgttta
    13681 ggtgtagaga tatatgctag ccgtaaacta ggtgcaataa tttttgataa actcgtaata
    13741 taaattgttt gattgggcag ataggaagcc actggattta aatgtgtttc attaagtaaa
    13801 ctatgaatag catcctcaat aacaattaaa ttgtatttag atgcaatatt tgcaatcatt
    13861 tttcgtccat tttgtgacat tatatgtgta gttggatttt gataatctgg tattatgtat
    13921 agacctttaa tattttcatt tttacaagca tataatagtc cttcttcact aatctcatta
    13981 tcttcttgtt taattggaat tagttgaatc ccaagcattt tagctgctgt ctttattcca
    14041 ggataagtta atgggtctac acctattcta tctccatgtt gaaacaaacc agctaaaata
    14101 gcaacaattg cattttgtcc accacttgca ggaagtaagc tatctgatgt agtttcaagc
    14161 ccagctttat aaattaattt ggcaatagct tctttttgcc atttagcacc atctctttgt
    14221 ccatattgga atatggtttt aaaatctatt tccttaatta tgtgtttaaa caaagtattc
    14281 atttcttcta gtgtgaaatc tggattcatt gtgcccattt caataaatgt aattttatta
    14341 ttactggaca tcaagaatag atttgaacgt gtatcataag atacaaaagt tccacttcct
    14401 gtaactccac ttattagacc ttttttttca caaattttaa aagctctaga gatagtactc
    14461 acattaacat ctaagaaatc tgccaattca cgttggggtg gtaatttagt acctggtaat
    14521 aaaaatccat tgttgatatc ttgttctagt tgttctgcaa gcgccttata taatatttgc
    14581 ccttttgaag gtctctttgg tttccagttc ataggataat tttcaaaaga attaattggc
    14641 ataagttttc acctcacatt attaatttga ttttttcttt atctaaaaca ataaattcta
    14701 aagatacaaa taaaaactga taggaaataa ttctatcagt ttttgtcttt taattgattt
    14761 caaatcttcc tataattttt ataccttctt tatcataaag agggattgtt tttttaaata
    14821 tattaaaatt tctttttctt gtatataaaa cagcttcttc tggtgtcttt ggttgattat
    14881 cacaatcgta caaatctctt ttttttacgt agccgacttt tttacctcta cctattgttt
    14941 gtataagttc tggttcaatg gaatttccat ttgagtctat tatatgtgta ccataggttt
    15001 ctccattttt attataatta atcttaccaa tagtatattc tacatcttca ttttgtttaa
    15061 aattggagtt gatagaaaat attgacccta ttattataaa ggttaataaa aacttaagac
    15121 ctaaattaat tttcttttta tcttgattaa acatagaatc taacctcttt tttaaaataa
    15181 tatttttttt atttagaaac tgagatacga atgatgaata cttcaaagat ttttgatact
    15241 ttattgtatc taatattata tatgcatact ctttaatttc actcgtggta cacttgtcaa
    15301 tcacaatttc atcacaagat aattcacata attcatgaaa atattttttt aataagtata
    15361 aaattggatt gaaccaatat atgatacaaa ttatactaat tagaagctta aataagttat
    15421 cttttctttt ataatgaatt aattcatgtt taaagatgaa tcttaactct ttattattgt
    15481 actttttttc tggaattatt attttacagc gtgttaaacc tataatggca ggagaataaa
    15541 tacctttaac cttatatatc tgaagttttt tatttatact tagttctttt gcttgttcat
    15601 ctaagatact agtaatatct atttctaaaa tttctttttt aaaattttct atgtaattat
    15661 aaaatttaaa atttgttctt aaatagtaaa aagagattgc tataattcct aatatccaga
    15721 tgtacataaa tatatctaaa gtaatgccct ggtcatatgg tatataatta tcgatattat
    15781 gcttatagat attaatagta gtattattga tatttaatat atttaaattt attttaga
//
LOCUS       DAYATN010000088        15791 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.088, whole genome shotgun sequence.
ACCESSION   DAYATN010000088 DAYATN010000000
VERSION     DAYATN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.088"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            102..1292
                     /locus_tag="ACGI8A_002535"
     CDS             102..1292
                     /locus_tag="ACGI8A_002535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFH0180527.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1462..2424
                     /locus_tag="ACGI8A_002536"
     CDS             1462..2424
                     /locus_tag="ACGI8A_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFH0180528.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2546..3208)
                     /gene="zmp1"
                     /locus_tag="ACGI8A_002537"
     CDS             complement(2546..3208)
                     /gene="zmp1"
                     /locus_tag="ACGI8A_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HFH0180529.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3308..3393)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3579..6497)
                     /locus_tag="ACGI8A_002538"
     CDS             complement(3579..6497)
                     /locus_tag="ACGI8A_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HFH0180530.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     ENENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNLGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(6615..6700)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6827..8005)
                     /locus_tag="ACGI8A_002539"
     CDS             complement(6827..8005)
                     /locus_tag="ACGI8A_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFH0180531.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            8380..8565
                     /locus_tag="ACGI8A_002540"
     CDS             8380..8565
                     /locus_tag="ACGI8A_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFH0180532.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(8630..11404)
                     /locus_tag="ACGI8A_002541"
     CDS             complement(8630..11404)
                     /locus_tag="ACGI8A_002541"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFH0180533.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(11576..12832)
                     /gene="glyA"
                     /locus_tag="ACGI8A_002542"
     CDS             complement(11576..12832)
                     /gene="glyA"
                     /locus_tag="ACGI8A_002542"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFH0180534.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(12904..14406)
                     /locus_tag="ACGI8A_002543"
     CDS             complement(12904..14406)
                     /locus_tag="ACGI8A_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFH0180535.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(14497..15687)
                     /locus_tag="ACGI8A_002544"
     CDS             complement(14497..15687)
                     /locus_tag="ACGI8A_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0180536.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT         4954 a         2735 c         1924 g         6178 t
ORIGIN      
        1 tatattttca aacaagtaat ttttcgaaaa ttgtctaaat tcagtaaaaa tatggtaata
       61 tatacataag caatataaat ctataaaaag gagtgttaca tatgatatct aataaaatgc
      121 aaactttagt tgcaaatagt tctgttataa gagctatgtt tgaagaagga aagaaacttt
      181 ctgatattta tggtgaggaa aatgtttttg attttagtat aggaaatcca agtgttgaac
      241 ctccagagac tattaaagca gttataaatg atattttaaa tgaagaatct ccaaatttag
      301 ttcatggata tatgaacaac tcaggatatg aggatgtaag agatgctatt gctgaacaca
      361 taaataaaaa agatggatta aatcttacaa gagaaaattt aattatgact tgtggtgctg
      421 ctggtggtct aaatattatt ttaaaaactc ttttaaatcc tggtgatgaa gttattgctt
      481 ttgcaccgta ttttggtgag tataaaaatt atacagaaaa ctatgatgga aaattaatag
      541 aagttcctac aaatatagag acttttgaac cagatttaga tgctctaaaa aatgctatta
      601 ctccaaaaac aagagcttta ataataaaca ctccaaataa tcctactggt gtcatatatt
      661 ctgaagaatt attaaaaaat cttggagaat tattggattc aaaacaaaaa gaatttaata
      721 caagcatata cctaatttct gatgagccat atagagaaat aatttatgat ggtgccaaag
      781 ttccttgtgt actaaaatac tatagaaatt catttatagg atactcttat agtaaatcat
      841 tatctctacc tggtgaacgt ataggatata ttgttgctaa tgggcaaatg gatgattttg
      901 atgatgttat gtcttcatta aatgttgcaa atagaatact tggatttgta aatgctcctt
      961 ctctttttca aagggttatt gcaagaagtt tagatgcaga agttgatgta aatatttata
     1021 aaaagaattt tgaccttcta tataatagtt taatcgatat gggatattct tgtgttaaac
     1081 ctaatggtac attctatttg ttcccaaaag ctccaattga agatgataaa aaattctgca
     1141 atgatgcaaa acaatttaat ttattattag taccaggttc ttcttttggt tgtccaggtc
     1201 attttagagt atcatattgt gtatcttatg ataaagttaa aagttcttta ccagcatttg
     1261 aaaaattagc caaattatat aatttaaaat aactaaatca aagtaattca aataattaac
     1321 taccttagta tagaatactt acattctata ttaaggtagt ttataatttt atgtactata
     1381 ttttatacat accatattat tccaatatgc ttgaatattc ttatctatag tactaatata
     1441 aattacaagg aggtgtttga cttgaaaaaa ggttatgttt atataatact gactacctta
     1501 ttttttagtt caatggaaat tagtctaaaa actgtcacta atgactttaa tcccttacaa
     1561 ataactttat caagattttt tgttggcggt ttagtcttac taccttttgc tgtaaaaaga
     1621 ttaagagctc ttagtctttc tattacaaaa tcagacttaa aatactttgc atttttaggt
     1681 tttatgtgtg tagtagtaag tatgtcattg tatcaacttg ctgttctaaa cacaaaggca
     1741 tctgtagttg ctgttttgtt cagttgtaat ccagtctttg taatgctttt agcttactta
     1801 attttgaagg aaaaaatata taggcacaat gtaatttcac ttatattaga agttcttgga
     1861 attatagtta taataaatcc attgcataca aaactaactc ttagtggaat attcttaaca
     1921 ttatcttctg ctattatttt ttcaatgtat actgtatttg gtaaaagaaa aaccttaaaa
     1981 tttggtggaa tagttgtaac ctgctttagc tttatatttg gtagtttgga aatgttgata
     2041 ttggttttac tcactaaaat taattttatc gcagatatat taaataaaaa taagcttcat
     2101 gtatttgcaa atatccctat ttttagtgga tataccttac acaatatgcc cattatgatt
     2161 tatgtattcg tatttgttac aggtgttggt tatgcgcttt actttatggc aatggaagct
     2221 acatctacta gtcttacatc tttagtattc ttctttaaac cagtaatatc tccaatacta
     2281 gcattattaa tacttaaaga aattatccct ataaacatgg ttataggaat tttattgata
     2341 gtaattggtt caataatttc tattatacct acgataatta ctcaaatgca taataaacac
     2401 gttgaagcct tgtataagga gtagtaattg ataatctatc atagttatac ataagacaaa
     2461 atttattaga tttaaacgca atataaaaga gatgctatta taatagcatc tctttattta
     2521 tatttatttt aaatttatta aaactctatt tagctaaatt ttgcaaaaag ctataagttt
     2581 gtggacaagc tgattttaac ttactatttg tatcttgatt taaatagtaa tatgcaaatg
     2641 attctgcaaa aaattcttct ggatatacac ctaagtagtt tacattacct aaagaacgac
     2701 cttctttagc aaatatttgt ttgaattgtg ctgatttaga tatatcattt aatactatat
     2761 ggtctattgc atgtgctgtt tcatgtaatt ctaaatttat tgcatcatgc cctttacctt
     2821 tgttactaaa tcctattcta agagctactg tacttccacc taaacctgga acatcatccc
     2881 aagtttttcc agtaccttcc catcccttag gaactacacc ttttaaataa gcatattctt
     2941 tttcatctgt taactttcca cttaataatt ttatttttaa gttattttgc tttaatgcat
     3001 ttaaatactt tccatctata ttagctaatc tatttaccat agcattagct tcgtttttat
     3061 cgtaatttcc agttggaaaa actactattt gactaagtgt gtctttattt tgttgtatag
     3121 tagtactatc tgcatgtgca cttactggaa ctgaacttat taagaatatt gatattatag
     3181 ctattaataa ttttttactt ggtctcatat tattccctcc tttgagttag gccgggagta
     3241 ataaatttca aaataaaaaa gatacccaag agtatctttg tatatacaaa aagaaatttt
     3301 tactcttttc ggcaactggc tggcatggtt tactaaactt tagcccctat agctttgcgt
     3361 gactaggttt ccctagtttt gcctcattta attttatatc tatattatag cttatatctt
     3421 tttaattgtc tatatccctt tggaactttt ttctaaaaag ttattatttt tttacataaa
     3481 tcttcaaaaa aaaatatctt ctaacaatat tttcattatt aaatattgtt ttagaagata
     3541 ttttttaata taacttatat tttttataat gttagggtct aatttgtatt tttatttctt
     3601 cttaatacga taagtcctac aatagctcct ataaccaaaa gtatttctcc aataattgta
     3661 gtactatccc ctgtttttgg tggtactggt ggatttacca aagtatcatc tttaacttta
     3721 ttattttttt cttctgacac tgatggcttt actacttctg gtattggaat aatctgttta
     3781 acaggagttt ctatcatctc ttctgatggt ggtactggcg gattcaatac ctctggtatt
     3841 ggcggattta ttatatcttc tgatggtggt attggtggat ttataactat agtatcctct
     3901 gtatcagatg aacttgatgg tttccttggt ttatctgtgc ttggtggtac tggtggattt
     3961 actattggtt catctgtatt tggtggtgct ggtggattta ctggtggatt ttcttgtctt
     4021 atgttttcta tatttccaat atccactatt ctaccaatat ctttattacc aatttcaacc
     4081 ttaattttat ccttcagttt ttcatatcca cttggagcca ttatctccac taaataatat
     4141 gttcctggtt tttttatact agattgaatt ataccatctt catcactaac taaatcctta
     4201 actactatat ttccaaatgc atcttctaaa ttgaacttag cacctgaaag tacattttta
     4261 tctaaagcat ccactttttt agcttcaaaa gcataacctt gcttagtagt ctctactcct
     4321 ggaggacttg aaccattaat ctgagccaca tttcgtatag tctccccaac ttttacattt
     4381 gtgaagtctg catcaaatgt aacccaactt tgcacaggtt ccccttcttt tatctgttta
     4441 aattcgtatt tatttactaa tcttattgta ttttttccat ctccagcttc ttttttaagt
     4501 tcaagattat cagaatattt tatattagat atttctagag ctgagtttac ttcatctaaa
     4561 agatttactt gtcctgcatc ataaacccca taagttgaca tattgatttt gtatttaaca
     4621 atctggttat cactactatt taaaactttt ttatcaactt ctttttttgc gcttattaat
     4681 ccagaatttt tcttaaagtt tatctcatca tttttatcaa tttcactatt atttgcatca
     4741 agaacagctc tatttatata gctatcttca atactaatta cctttaatgt ataagctata
     4801 aaaaactttg attccacttc ttctttactg agattaattg taaatccagt tataacttta
     4861 tctacttgcc cactttcatt tcttttttct tctactttat atgaaatatt attggcaatt
     4921 ttttctttac tccaaggagt ccagctccca ttcattggaa tttctacaac tctaaaatca
     4981 tgcccagctt ctcctgaagt agttaatctc atgccttctg gtatattatc tgttataaca
     5041 acattactta ttggtgattt atcatcattt attgaaatct gatagtttat tacttgacca
     5101 acttgagtaa tatttgttgc tcctgaatgt cctttagtaa gaagataatc atctcctcta
     5161 tcaaatatac ttacagtagc atcatcttct tgtatcttct taccatcaga ttcaatccta
     5221 gctacattct tatatttatt accatgtttt gcatttttgt cactgattct agccttatac
     5281 ttaacagtat acgatttatc tatatttcca agattaactg aaatagaatt tttactctgt
     5341 tcaatattaa aatcactaac ttcttttcca tcacttgtta ctacacttac agaaccattt
     5401 atcaatgtca atccttcagg aagctcatca tacaaaataa tatcttttaa ctcttcctgc
     5461 ttttcattta cttgtatact gtaatttata tctttatttt taactggcat aaatacagta
     5521 gttgattttt ttccttcaac aagcttttta agtgttcctt tagcatctgg aacatccgtt
     5581 ttatctccag gttttatttc tacatctata tctccacttc cactttctat ttttccagta
     5641 gtttcaatat gtatttttgc attagtatct tcatcaacct ttttaacata tgcttccaaa
     5701 cctattttac cttgcattac actttggcca ttaggtaatt ctctaaatgt aagtgtatac
     5761 tctccattac tttctgtttt atcaaagcat tcacttgtat aatctaaatt aactttattg
     5821 aatacatctg gcaccttaaa agttattgaa tctcctgctt gtattccttt tgatgttgga
     5881 gtataagtta aattaaatga tatttttttc attgattccg tttctattgt attgttatta
     5941 atttttacaa caaatccata gtcttcattt tcttcattaa gttcttcttc taaaactttc
     6001 aataaatttt cattttctaa tagattatct tcctctggag ttttttctgt atttttattg
     6061 tctagtggtt tttcatcttc taaagcttgt tctgactttt tattatctgt tgacttatct
     6121 tcttctggta tctgctctgg ctttttatta tctattggct tttctccttc tggagtttgc
     6181 tctggctttt tattatctgt tgacttatct tcttctgggg tctgttctgg ttttttatta
     6241 tctgttggtt tttcttcctc tggggtctgt tctggttttt tattatctgt tgccttatct
     6301 ttctctggag tctgttctgg ctttttattg tcgctttcct tctcctctcc agttgatgaa
     6361 ttagttgaac cactttgaat ctgactattc tctcctaatt ccgaagcata agatacagtt
     6421 ggatatattg tagacaccac catacttaaa atcataatta aactaattaa taatgccttt
     6481 ctatttcctt tcttcatgta aatctcaccc ctttaggtta cctataattt ataatctaat
     6541 cttccattaa tatcgtatat aaagtaattt ttactaaaaa attatacaaa aaaagctatg
     6601 aaaataaata aatttatatt catagccggt tgcaccactg actcagtata tccataaggt
     6661 gcccaataat agaatatact tcaacatctc tataatattt cataaaaaca attaaaattg
     6721 ctttatttat attaaaaata caattatata cttctattat attatgcctc tatactattg
     6781 tctatccttt ttacattttt taccttatga tgagattaat taataattaa ataaaatcct
     6841 tatctacaac aattacacaa ttatattcag gatttatctc cttaactaat tttgttatat
     6901 cttcttttaa gtcatcttct gacatgtttt tggaaaattc ggaaggatta actactatat
     6961 caaatattaa attcttcttg cttccttttc caactattct aaaatcatgc atagatttta
     7021 caagactatt tttcttaagt atttcttcaa gttctttttt ggctgacata acttctttat
     7081 cttcaagaca tattgggtcc atgtgaatta ctagatatat atctaatttt tcagatattt
     7141 ctctctctgc agtatctata atttcatgta tttccattat atctatgttg gatggaattt
     7201 ctgcatgtat agatgcaata catctaccaa ctccatagtt atgaataatt aaatcatgtg
     7261 tacctattat atatttataa gataacaata gctctataat agaatttacc atttcttcat
     7321 ccggtgcttc ccctaaaaga ggatttatag tatcttttac aagagagaaa ccagcataaa
     7381 gtatcactaa tgatactacg ataccaacat atccatctat gggaaaatta gtaaatctag
     7441 ctacaataaa tgatataact acacaacttg aagtaaatac atctcctaaa gcatcaagtg
     7501 aaactgcttt taaggctgaa gaatcaattt tatttcccat aaatttatta aaacgactca
     7561 accagatttt aatcattata gatgcaatta aaagtataag tggtattact tcaaatttta
     7621 caggaattgg atttactatc ctttctatag atgactttac aaattgaagt ccaaccaaca
     7681 tcaccataaa tgctactatt aatgctgata aatactctat tcttccatga ccaaaagggt
     7741 gttctttatc agctggctta cttgctaatt taaaacctat catagttatt gctgaagatg
     7801 ccatatcaga aagattatta aatgcatcag ccattatagc tatactagat gtgagcattc
     7861 ctatgaatac ctttatcaca aataaaagta aattggataa tatacctact acaccagcta
     7921 cataaccata cttatttcta acatctgtat tttgaacgtt ttcgctatct ctgataaatg
     7981 ttttgactag gattttggaa aacatgaaac ttacctccct gccctttatt ttattaacaa
     8041 attttataat ctatatcttt atttatgata acataattta tatataaaat tcttaaaaat
     8101 cactctactt cagtaaaata ttattctata tatctattta gatacgaatt ttatatttag
     8161 taaagttttt atggcttagt gtctgtttaa tagattctat ataagttttt acaaagatta
     8221 tttattatga taatatcatt tttcccaagt attacttaga atatgatttt tttgttttgt
     8281 ctgtacatat tttaaattta tactatattt atctatattt tataacctta tgatacacta
     8341 taactataag attttaaatg gaaaggaatg aaataaatca tgttttttat cagtgatatt
     8401 tacacaaaat cacctattaa atttgatact cctttgcaaa aagaggcata taaaatatta
     8461 caaaaattag atattgactt tgaatgtgtt gacacagatg aagcaattac aatggaagat
     8521 tgtgttcaga ttaataaaaa attaaatatg aaaatggtaa tataaaacaa cattaaacaa
     8581 acaaaaaccg caaagttaca atatatagta ctttacggtt tcataagaat taatttttac
     8641 tattaaagtt catagttcta ctatttttca ttgttctaag aattagcttt acaaattcat
     8701 ttgacaatag gatagtggat gcaaatagta ttatctttat ccacatatca aagtccaaag
     8761 aaacagaatt aaagaaactc tggaacactt gtgtaaatat tatttgaatt atccctgtaa
     8821 ctactattat ttgcaatgcc agtttatttt taaagaaatt tggtattgta ctatttaaac
     8881 caaactctct acaatttaat gcattaaaaa gggcactgaa tgcaaataaa gaaaatataa
     8941 cagtaccttg ttcttctgat gtagctccca atatattaaa agctgattga gtaaatacaa
     9001 ttgtaattat aagaattgca tttataataa tatttacaaa catagaccta gctattattc
     9061 cagaatgtct atttattggt tttcttttca atacataatc tctaacaggt tctaatccta
     9121 atgctagggc tggaggacca tccattatta tatttaccca taataattgt attgtagtaa
     9181 atggcatatc cttaccagta agttgagata ttatagcaat tataaatgct actatattta
     9241 cagtaagctg aaactgtata aatctttgaa agttctcata tatacctctt ccccatttta
     9301 ttccctctac tatagtacta aagctatcat cagtcaatat tatatctgct gcatttttag
     9361 atacttctgt tcctgaaata cccattgcaa ttccaacatc tgcttgacta agtgctgggg
     9421 catcatttat accatcacct gttacagcta caacttcgcc acttttttga agtgctgaaa
     9481 ctattctcat ttttgtatct ggtttacttc ttgcaacaat agatattcct ttgatttctt
     9541 ctctcaattc tttatctgtc aatacatcta tatatgttgc ttcaactgcc tttttcccat
     9601 catttaataa tcctaattca tttccaatag ctgttgctgt atttatatta tctcctgtaa
     9661 gcatttttac atctactcct gcattaaatg ctttatcaat ggaatctttg accccttctc
     9721 tcaatgggtc cactattcct acaaatccac taaaaactaa attatcttcc ttcatataac
     9781 ttcttgaatt tcctattaaa ttcaattcac cttctgaagt cattgcaact tctgttttag
     9841 aattactcat ttttttataa gcaaatccta atgttctcat agatttaatt tgtaacttct
     9901 taatctcatc taaaatactt ttttctactt ttggagttat tggaacaata ttttttcctt
     9961 gttgtacata agaacatttt tttaataata cttctggagc accttttgaa agtaatatat
    10021 ctgaatcctc ttgacgaatt aatgttgaca tcttcttttc ttctgaacta aatggtattt
    10081 gtgatatcaa atacgtttgt tttctcattt cattatagtt cttatcatta tgatataaaa
    10141 gtaatgcaca ctctgttgcg cttcctatat atttaaatga attatcttct ttttctatat
    10201 ctgcagttga atttactata cagttttgtt caaaatagct attactttga tactctcctc
    10261 cacttatata tttaccatca acatatgcta cctctaccat cattttgttt tgtgttaaag
    10321 tccctgtttt gtcggaacat attacagaaa cagaacctat agtttcacag gcttctttct
    10381 ttgttactaa tgcatttatc tttgccatct tttgcattgt tatagctaag gtaatattta
    10441 tcatagttgg caaaccttct ggaacagttg caactattaa tgcaatacaa accatatatg
    10501 catttttagc aggttctaat gattgtaaga aagataatat actgcttgta tctaaaatta
    10561 acataccttt tctctgcatc tgtataatca taaacataca caataaagta gctattatgc
    10621 ctgatattcc tgctatttta gcacccaact tccctaattt aatttgaagt ggagtttgct
    10681 ggtcttcatt tgataaattc tgtgctatct tacccatctc tgtcttatca ccagtatgag
    10741 tgacaaccat tatgccccta ccatatgcta caagagtccc tccaaatacc atattgactt
    10801 gctttgcagg tattgcatcc tgttcgatta tttctgtttt agaataaact acttccatat
    10861 ctaatacagc atcagcattc ttagctacat catcagattc tcctgttagc atatcttctc
    10921 taagtttcaa atttatagac tgtataagtc ttccatctgc tggtatcata tccccagttt
    10981 ctatatatac aatatctcct ggtactaaat cattttttga tattttaatt atcttcccat
    11041 ttctaagcac ctttacttct atattttctg ttaattttga cagggcatga gctgcttttt
    11101 tagattttcc ttcagttatt atgcctatag aaattccaat aaaaatagcc cctaaaatac
    11161 ctaatgcgtc atgtaattct cccacaaaag aacttattac tgctgctcct atgagaatta
    11221 atatcatagg ttctgtcaaa ctttctccca actcgtccca aaatgttcta ccttctttta
    11281 tagtaaattc atttaatcca tatctaagct tcctttcttc tacctcatta tcatcaagtc
    11341 caatttctgg atttgttttt aagtatttta atacttcttt ggtaggttta ttataatacc
    11401 tcataacttt atcccccttg ttaattatct ataaggaatt atatgcttat gatagcttgt
    11461 atattacttt tcaaaaaaaa taaggtgttg attaatcaac accttatagt ggttatatat
    11521 tattaaattt tatgttacag gctttataaa ttgtgagcta tacaaatcaa aatatttaat
    11581 cttcaaaata cccttctgga tataatggaa acttagatat aagttcttgt gcctctgctt
    11641 tacattcatc taatacttct ttattatcaa tattttctgc taccttattc attatccctg
    11701 ctatctgtat aacttctttt tctttcagac ctctttgaga tattgatgta agacctatac
    11761 gaacaccact agttacaaat ggtgattgtg ggtcgtcagg tataacattt ttattaactg
    11821 ttattcctac atattcaaga gctttttcaa attgttttcc tgtaagattc ttacttctta
    11881 agtcaaccat tactatatga ttatcagtac ctccacttac aatactaaaa ccatgtgaaa
    11941 tcagttcaga agctagagtc tgagcattta ctacaacttg ctccattatc tttttaaatt
    12001 tatctgtact taaatataat agtgaaaagg cttttgctgc aactgtatta agatgtatag
    12061 aacctaatgt tcctggaaaa acacctttat cgagtttctt tgcatgttct gctttacaaa
    12121 gtacaatacc acttcttggt ccacagaaag tctttgtcgt agatgaagaa acaaaatctg
    12181 cataaggtac aggactaggt attactttcg ctgcgactaa tcctgcaaca tgtgccatat
    12241 ctaccataaa gtaagctcct acttcttttg caacctttga tattctttca taatctatta
    12301 atcttggata tgaacttgcc cctgcaatta taagttttgg tctacattct tttgcctttg
    12361 cttcaagagc atcataatct atacgtccag tatttgggtc taccccataa aattcatatt
    12421 tataaatgtc atgtaaaaaa ttaactgcgc taccatgtgt caagtgtcca ccttggtcaa
    12481 gacgcatact taaaacagtg tcatttggtt ttaatattga tgaatataca caatagttag
    12541 ctgtagaacc tgaatacggt tgtacgttta catgttctgc tccaaatact tcttttgcac
    12601 gttttattgc tagggtttca agtttatctg cttcttcaga acctgcttga aaacgagccc
    12661 ctggatagcc ttcttctgtt ttatttgtaa atacacatcc acttaattca agtacttctg
    12721 ttggtgctgt actttcagaa gctatcatct caatattgtg ttcttgtcta accaactcat
    12781 cagctacaat cttgtataac tctgggtctg aaatttttgt tgtttttaac ataataatcc
    12841 tcctcttaat taaagaatat attttattag tttaatcttc attgataaat ttattataac
    12901 ggtctataaa acgtaagata ttcctccacc aggtcctaat ggtatgccaa tacccatgaa
    12961 tagtaacaat attattattt ctattaatgt aaataccact gagtatggaa tcatattcga
    13021 tatgacagta cctataccta tatctttatc atatcttctg gcaaaggcca atataactgg
    13081 aaaatacgga aaaagtggac ttattggatt tgttgaagca tctccaatac gataagctat
    13141 ttgagttaat gctggattaa agtctagtag cataaacatg ggaacaaata taggtgctaa
    13201 aatcgcccat tttgcagaag cacttcctac aaataaattg ataaatccag atagcaatat
    13261 aaatcctatt atcaatccat agctaggtat tcccgactct gctagtaatt ttgctcctgt
    13321 tattgataaa attgttccta agttactaag attaaataaa ttaataaact gggatgctat
    13381 aaatgctagg actatatatc cacccatatc actcatacta ctacccatca tagatactaa
    13441 atctttgtca ctttttatct tcttacttac ttttccatac actaatcctg gagttaaaaa
    13501 tataatagtg attataggaa ctataccttt cattaaaggt gcattactag atagtataga
    13561 accagtctct ggatctttca taaatgcatt gtcacctata cataaggcaa ctatcactac
    13621 tactacaact aacaatgata ataaagcgta ttttataccc ttattttcaa gttcactcaa
    13681 ttcctgtata tccttatcat ttgttgaatc attatatttt gtaaaacgag gagctataaa
    13741 tttttcagtt acaaatgctc ctgcaaaagt taaaactatc gctgatataa aagcaaaata
    13801 aagattcatt gctggattgc cttggtaaga tgggtccatc atttgagctg ctggtattgt
    13861 aaatccagct aacaatacat ctgtagtact aagcataaca tttgctgcaa atccccctgc
    13921 aacacctgca aaagctgcaa acatacctac aagaggatgt cttccaattc ctaaaaacac
    13981 aagtgctgca agtggtggta atattataaa tcctacatct gctgctgtat gtgacaacat
    14041 acctgcaaat acgattgcta cagttacaag tttttgtgga actttactta tacccttttt
    14101 cattaacact tccataaatc cagatttctc agcaacacct gctccaagca ttgtaaccag
    14161 tactaatcct aaaggagcaa atgactgaaa gtttgtaact atgctaccta aatatgattg
    14221 aatctgctct ttctctagta gatttactac ctttacaact tcttttgtag aaggatgtat
    14281 ttcttctact cctaaattag atataactgc tgacaatatt acaacaatta tacatagtaa
    14341 taaaaatatg gttactgggt ctggaagttt atttcctgct ctctctacaa catttagtgc
    14401 cctcatcaat aatccactat ctttgttttt ttcctgtgaa ttaattttcc tcaaactaat
    14461 accccctcat aaactttatc tccagaatgc tctatcttaa attttatttt ttaatttatc
    14521 catataattt ataacagact gacatagtac atttgtacct accaatatag aatttttatc
    14581 agctttaaat ttaggattat gccactgata tatacaatct tgttctttgt tgcctacccc
    14641 taaccagtaa aaaaatcctg gggctttttc catgaatata gaaaaatctt cccctcctgt
    14701 agaaggtatt ggatcaacta tagcatcttc tgaatacaaa tcacacacag acttacaagc
    14761 tattgcgtac atctctttac tatttataac agctggtaaa tcaaataaat aatctagctt
    14821 tgcagttgct tgatatccac gagctatctc acaaactata ttttctaaat tttctgatat
    14881 tttctttcgt acttcattac taaagcttct acatgtgcct gtcatatcta ccttttctga
    14941 aattacatta tttgcagtcc ctccatttat agtacctatt gatataacag ctgattctaa
    15001 tggacttatg tttctgctta ctatggtttg aatacccatt ataattgcac ttgatgctac
    15061 tatagggtct attgttctgt tgggtatacc tccgtgacct ccaaatccat ttacttctat
    15121 cttaatagtg tctactgctg ccattaaccc acctaactta acacctattt ttccacaagg
    15181 tatatctgga tgattatgaa gtccaaatat agcatcaact tttggatttt ccattactcc
    15241 tcgttccact agcatcttcg cacctttatt gacttcttcg gctggttgaa atataaactt
    15301 tacatttcca tgaagactat ctctaatatg agacaataca tatgcacatc ctacaaggca
    15361 agacgtgtgt atatcatgcc cacatgcatg cattatacca tcatatctgg atttatactt
    15421 aatatcaacc tcttcatata ttggcaatgc atcaatatct cctcttagtg ctactgtggg
    15481 accatcatac ttccctttta ataatccaac aactcctgtt tccattccta aatctaatat
    15541 ttctatgtct aaactcacta ataaatcttt tatagctttt gtagtcctaa attctttaaa
    15601 acttaattcg gggtgcatat gaatatctgt aaaaaaatct aaaatatctt ctttatactt
    15661 ttctgagatt aaaataagtt catgcatact aacacctctc caaaatttta tttttttatt
    15721 ttttataatt taactacaaa ttatataagt taataatctt tataaagata ttttttataa
    15781 atttaatttt a
//
LOCUS       DAYATN010000089        15763 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.089, whole genome shotgun sequence.
ACCESSION   DAYATN010000089 DAYATN010000000
VERSION     DAYATN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.089"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            359..1381
                     /gene="ccpA"
                     /locus_tag="ACGI8A_002545"
     CDS             359..1381
                     /gene="ccpA"
                     /locus_tag="ACGI8A_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HFH0180537.1"
                     /translation="MKMKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEV
                     IKETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANT
                     YSDKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYT
                     VSTSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKS
                     LIKYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVA
                     GFNDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRES
                     VTERKK"
     gene            complement(1504..1902)
                     /locus_tag="ACGI8A_002546"
     CDS             complement(1504..1902)
                     /locus_tag="ACGI8A_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180538.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            2114..3025
                     /locus_tag="ACGI8A_002547"
     CDS             2114..3025
                     /locus_tag="ACGI8A_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180539.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDYDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            3140..3320
                     /gene="ssrS"
                     /locus_tag="ACGI8A_002548"
     ncRNA           3140..3320
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGI8A_002548"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3503..4720
                     /gene="cdeC"
                     /locus_tag="ACGI8A_002549"
     CDS             3503..4720
                     /gene="cdeC"
                     /locus_tag="ACGI8A_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HFH0180540.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(4821..6143)
                     /locus_tag="ACGI8A_002550"
     CDS             complement(4821..6143)
                     /locus_tag="ACGI8A_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFH0180541.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(6194..6988)
                     /locus_tag="ACGI8A_002551"
     CDS             complement(6194..6988)
                     /locus_tag="ACGI8A_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HFH0180542.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            7098..8543
                     /locus_tag="ACGI8A_002552"
     CDS             7098..8543
                     /locus_tag="ACGI8A_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFH0180543.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            8572..8781
                     /locus_tag="ACGI8A_002553"
     CDS             8572..8781
                     /locus_tag="ACGI8A_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HFH0180544.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            8801..10195
                     /locus_tag="ACGI8A_002554"
     CDS             8801..10195
                     /locus_tag="ACGI8A_002554"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFH0180545.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVIWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            10435..11187
                     /locus_tag="ACGI8A_002555"
     CDS             10435..11187
                     /locus_tag="ACGI8A_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0180546.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            11213..11626
                     /locus_tag="ACGI8A_002556"
     CDS             11213..11626
                     /locus_tag="ACGI8A_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0180547.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            11630..12373
                     /locus_tag="ACGI8A_002557"
     CDS             11630..12373
                     /locus_tag="ACGI8A_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HFH0180548.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            12373..12843
                     /locus_tag="ACGI8A_002558"
     CDS             12373..12843
                     /locus_tag="ACGI8A_002558"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0180549.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            12860..13618
                     /locus_tag="ACGI8A_002559"
     CDS             12860..13618
                     /locus_tag="ACGI8A_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFH0180550.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            13602..14444
                     /locus_tag="ACGI8A_002560"
     CDS             13602..14444
                     /locus_tag="ACGI8A_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFH0180551.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(14604..15509)
                     /locus_tag="ACGI8A_002561"
     CDS             complement(14604..15509)
                     /locus_tag="ACGI8A_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HFH0180552.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT         6139 a         1779 c         2738 g         5107 t
ORIGIN      
        1 agattgctgg agagacattg acataatgtc atggatacct ttcttaatat tattgctaat
       61 aacttgttca tgtggtggat tctttgatgg tgaagtaggt tgcggctgct aatacttaaa
      121 tcattagaat aaaaaaatgc tggtatttta taccagcatt tttttatttt attttttaat
      181 atgatttaaa ttaagaatga atattattaa atttgttgaa tatagggtat aatataaaat
      241 ataatgtatt taaatgtact tgcttttatg gtaaaataaa aacttatata aatgtagtta
      301 aaataggaac cttcaaaaat ttggttcagt tttagataaa tttagtaaaa taaggaggat
      361 gaagatgaaa ggcaatataa cgataaaaga tgttgctaaa caagcaggag tgtcaatatc
      421 tactgtatct agagttataa atgattcaaa acctgtaact gatgaagtca aacaaaaagt
      481 tttagaggtt ataaaagaga ctggatatat accaaatcca cttgctagaa gcttagtaac
      541 aaagaagagt caattaatag gggtaatagt tccagaagtt tcagattctt ttgttaatga
      601 ggtgttaaat gggatagaag aggttgctaa aatgtatgac tatgatattc ttttagcgaa
      661 tacatactct gataaggaac aagaacttaa gagtataaat ctattgagag caaaacaagt
      721 ggaaggtata gttatgattt catggatagt tgaacaagaa catatcaact atatacaaaa
      781 ttgtggaata ccagcgacat atataagtaa aactgctaga aattatgata tatatacagt
      841 aagtactagc aacgaagaag ctacttttga tatgacagag catcttataa agaaaggtca
      901 tgaaaagata gcttttataa tgacgagtaa agatgatact gttttagaaa tggaaagact
      961 tgctggttat gagaaagcac tttcaaataa caatatagaa ttagacaaga gtttgattaa
     1021 gtatggtgga actgattatg agagtggata caatagtatg aaagaactat tagatgatgg
     1081 aataatacct catgcggctt ttgtaacagg tgatgaggct gccataggtg ctataaatgc
     1141 tatatgtgat gctggatata aggttccaga agacatatct gttgcaggat ttaatgatgt
     1201 taagatagct agaatgtata gacctaaact tactacagta tatcaacctc tatacgatat
     1261 gggagcagta gcaataagaa tggttataaa gttaataaat aaggaattaa ttgagaataa
     1321 gaaaatagaa ttaccttata gaattgttga tagagaaagt gttacagaaa gaaaaaaata
     1381 aaactattaa aatcaatcag gtagtgccta aggcactacc tgatttttta tgtcaataat
     1441 atcaaattta ataatagatt aactaaaaat aattatctaa ttacttaaat agtttatttt
     1501 tatctattca tgagggttag actttttagt tttcttaaat aaacttttag attttttttg
     1561 ttgactacct ggtgtatttt ttggtgcagg tgaatttgac ttacctttag taggtttagc
     1621 cttcttagtt ttagctggtt tagtatctct ttggttttct gcatctgaga ttttttccat
     1681 tatattatcc agttttttgc gttgattttc atctaaaaat gctttgaatt ttgatactac
     1741 ttcatctctg ccatctaggt tcttacctat ttttataagt tcctccataa gttcattttc
     1801 tgattttcct ttgtaactat cagccatctt ttctatttta tccttgtcaa tatttttttg
     1861 tttagccatt ttttctaatg actctgtatc taattttttc ataaaaaaac ccctttctat
     1921 aaatatatat tgaattatat tcatactatt tatatataga aataatattg aagtggtgaa
     1981 ttgtgagtga tatataatat aaataaaaat tcttatagat aatatatatg cactgagctt
     2041 taaaaatgga acattaaaat atgaaaaaca tactttaata tagggcaatt atataatata
     2101 agggggaatt gtcttgaata acttactatt tttgattgga gtgattgcat tgagtaatgg
     2161 taatatctct atatttgatg ataaaaataa atcaaaagga aataatagag ttagaaaaaa
     2221 caaaaaaaat gcaaatgata aactttcaca gtctacacat ggcaatttaa ggtttgatgc
     2281 aaatgatata aaaagaggtt taaggctcat ggatttaagt gaagaagatt tagaaatggg
     2341 aatggaaata ataacaagaa ctaaaaaata catgtcaaga gatgaaagaa aaatactaat
     2401 aaaaatagaa agtgttttag atttagttag aggaataaaa aaattaaata atatagatat
     2461 ggtagataca gaagatgaaa cggatttttt cagaaaaatg gatagtgatg ataaaaagaa
     2521 tatgatgata aaggaaatta tagatgtatt cccagaaaaa agaaaaaatt cagttgaaaa
     2581 agctatagga atgaaaaaga agatagattt atttgcagaa ttatttttac cagatgactt
     2641 tggtgaaggt ggatttagtt tgagctcttt ggcaaatata aacaatcttg gtagtatgaa
     2701 taatctcaaa ttgttaggaa gtcttttaag aggtgatgat agtaataatg atgatgaaga
     2761 caataatgat gagttaaatt atgaagacga agaattagat tgtgaagaca aagaattgga
     2821 tgatgattat gatgagttaa cagacgaagg tgaaatggag gataatgaag atgaattagt
     2881 agaagataag aacacaggct atgaaaatat agaaaatgat gaagatatag tatatgatga
     2941 ggaactatac aacattaatg agcaagaatc cgaatacaat tataatgaaa aaaatactaa
     3001 aactagagct aagaaaaaac gataattaac taaatacata aagaaaaaag gaagaaaaag
     3061 taatgataga ataagattat ctcaattact atataaaacc actttaaaaa attcaaaatt
     3121 tattatataa taataatatg aaatcctgaa acatacgata aggctttttt aaattcaacc
     3181 tacaaatgaa atacgggaga ccgtgtctgg taaattataa catgcctagt tagtttggac
     3241 gttaaaggtt actggcaagt cacccacctg ggagtgccta gggtgtgaat ttacttagcc
     3301 accggtgttt cgggacaaaa aaagtgctac tatttcaaat agtagtactt tttttattaa
     3361 tatgcatttt tctactaaat acgcctaatt attgataaat tcaaaaagca agtaacaatt
     3421 aagattataa aagcatagta ttaattaggc ttcaaaaagc caaagactta ataagaatcg
     3481 cttacaggga gggcaatgaa aaatgcaaga ttataaaaaa aataaaagaa gaatgatgaa
     3541 tcagccaatg tctacaatga atgaagaaga agtgtataca gatgaaataa attcagaaga
     3601 catgagaggt tttaaaaaat cacaccatca taatggatgt aatactgata ataagtgtga
     3661 gtgccatgat gattgcaatc catgcaaccc atgtaatcca tgtaaaccta acccatgcaa
     3721 tccatgcaaa cctaatccat gtgatgacaa ttgtggatgc catgacaatt gtaaatgtga
     3781 ttgtgaacca tgtgaaatgg attcagatga atgttttgaa aacaaatgtg gaccagaatg
     3841 ctgtaatcct atatctccaa gaaacttctc tgtatcaaat gcagtgccat ttgcaataga
     3901 ggctaataga atatttgata ctatgcaatt ccaaacattt acagatgcaa caggaccaaa
     3961 tggagagcca ttaacttttg aaacagaagt agtagaagta tttggttcag ttccaagtgc
     4021 aggtcaagca agtgtaacta tagaaaaaat atgcttaagt aatgatggaa tcgttataga
     4081 cacaggaatg acaactttag aagatttcga tttagaccca ttaggagata tagtaggaag
     4141 aaactgtgaa acaacttttg aatttgcagt ttgtggagaa agaaacgctg agtgctgtag
     4201 acaaggaaaa ggcaaatcag tagcttataa acaaagagga ttaactgtag cagttcgtaa
     4261 tttagtacta gagctaagag gtagatgtgg atgtacagag ttcgttgcat tagctttccc
     4321 agcagttaga gcaggaggtg gatgtaagag aagagttgat tatgtagaat ttacttttaa
     4381 cacactttca gcaccaatat gcttgccagc tgacggaaga gctgttactt taagacaaga
     4441 atatcaaact aacttaactg tagattgtat aggaaaatct atattaaaat tagaatgcaa
     4501 cgaatgttgt gaacctttct atgaattaat tataccaaat gatatagatt tagtactttg
     4561 cttacaagaa acagttagca cattaataag tgaacaaata gtagttttag catcaccaaa
     4621 tccaatccaa ccaagacttg ttgatacttt ctctaaagta tgtgattttt cgcaatgtgg
     4681 acctaatcat ggaagtggaa agccaagttg ccacagatag ataggtaaga tatatagaca
     4741 gtttaaatta taaataaaaa tcatctaagg aaggctacca aagtgtataa tttacattgg
     4801 tagccttcac gtttttataa ctagcgtata gattttagtg atttaaaaag cactatagta
     4861 tgtaatagac aagtaaagat tgatactatc gtcattgtgt atatgtatgc ataaatatta
     4921 agtcctggaa ttggaagaaa tacatataaa gctattagtt gaattaacat gccagtaata
     4981 gtatttatac ttgaagctaa ttcttttctt attgaatgta aaatgccaga tagagtttga
     5041 tttagggaca taaatagtgg aactaaaaac atagctttta gatattctcc tgcaagtgta
     5101 tttttaaata ctaatataca taaatctttt ccaaagaaat aaaaaaatat tgatgatagt
     5161 atgcctacaa ataaagtaag aagtatggag tatttgattt ttttaattac actcttacgc
     5221 tttcttagag ccatttcttg tgaaatagtt ggtattaaat ttacaataag cgctgaacca
     5281 acagtaaaag gtaaaaatat aaaaggcatt accatcccac tgacaatacc atacatactt
     5341 aaagattgtt gataagtcat tcctgataat gctaatcttg atggtatcat catagaactt
     5401 atagattgca aaagtatgtt tgacattcta ttacaagtta tgggaataga catttttaat
     5461 gtttccatag aagaattata aaaatctttt atgcttataa tatacctatt gtcaaatgat
     5521 gaatctcgcc ataagcaaat aagcataaat ataatattac tcaactcacc gattgatata
     5581 cctaatagag ctatataaca attcatagat ttattattta tgtacataac tagtaaaaaa
     5641 acaaataata ttttaccaat ttgttcaatg atttgtccaa tagcagggat ttttacattc
     5701 tttatgccat agtaatatcc tcttagtacg tttgaaagag ttattataac tatagcagga
     5761 caaacagcta gtataaatag atttaattta gcatctttta aaaatttaag tgataaaaaa
     5821 gaactattga aagatgcaac tatagatata attagtgcta cgaaaaatga tatatataaa
     5881 gttgatataa ataaagcatt agtattgtgt ctatcgttta aagcttttct ctttgcaact
     5941 agacaactaa gtgcagtagg tatacctgtt gttgtaacag ctaagcaaat catcaaaaag
     6001 ttaaaaatca tatgatagat acctaaacct gtttcaccta gtgctcttga taagaatatt
     6061 ttatataaaa aaccaaatat tcttacaata aaattggata taaataaaat aactgttgat
     6121 agtactaaat aaggtacttt caaaaaaatc acctccttat cttttaaata tattcacaag
     6181 gaggtgtaaa aatttataat tttataaatt ctggtttcaa tttattaaat ttagatacat
     6241 atttaaatcc catatttttt agtaatgaat atatgtagtc aaactcacat gcaacttgac
     6301 ttatatgatg tgaatctgaa cctgtggtta tgatttcccc tcccaagtct ttatacattt
     6361 gaagtatata cttagatggg gttaggtctg gaagattata tctataacaa gaggtattta
     6421 tctcgatacc tttgccatca taaatagctt gtttaagagt ttcttcaatt atatcagaaa
     6481 atagtctatc atctaaaata gtgtcataag gtgcatacct tttaatcaaa tctaaatgac
     6541 ctaagacaga gtaatcttta taattcttaa caatattata taaataaaga tagtaatttt
     6601 cataaacttc atgttgagtt ttatctttaa aataattacc aaggtataag tccattgtat
     6661 ctatagcatg gatagaacat attataaagt ccaatggata ttgatgtgct tcctttttgt
     6721 atgtatccat taattgagtt tgaactccaa attcaattcc tttttttatt gatattttat
     6781 ctttatattt gctttgaaga gtctctaaac tcttaaaata atcttcgtag acaattgaaa
     6841 aagaatcatc agcatataca tcatagtcaa catggtctgt gaaacaaatt tcctccaaac
     6901 cgagttcaat agattttttt accatttctt ccattgtaga ttttccgtct gtagagaaac
     6961 ttgagtgcat gtgatagtcg taaaacataa atatttcccc tttttataaa aattttggta
     7021 ttatatatac atactaaata atatcaaata tacataaaag catcaataaa atataaatac
     7081 aatgagcggt gataaatttg gaaaaataca gaatagattt aaatgagaaa aaatcaacta
     7141 aatatataca gatatttaat catataaaag aattgataat aaataaagag ttagtagaac
     7201 atgaaaaatt gcctccaata agaaaactat caaatttttt acaagtaaat aacactacta
     7261 tagttaaggt atacgagctt ttagaaaatg aaggatatgt atataaaatt atagggagtg
     7321 gtacttttgt atcaagttta aattcttata aaagcggtat agataacagg aaaaataaga
     7381 ttattgtgca aagagaagat ttaattcatt ttgacaatgg gaatccatct aatgatatgt
     7441 ttcctatcga ttcatttaaa aatgctgtaa atatggcatt atctaaagat ggtagttcaa
     7501 tatttgaata tgatgaaggt cttggctctg aagaattaag agaaaaaatg gtagagtatc
     7561 taagtgatgg aaatataaaa actactaagg aaaatattca aataatatca ggtgctcaac
     7621 aaggtataga tatagtttgt aaaggactta taagttattc agatgttgtt tttatggaag
     7681 aaccttctta caatggagca attgaagtgt ttaaaagtag aggtgcaaaa ataatatcta
     7741 tacctatgtt agatgatgga attgatatag gtattttaaa attaaagttg gaaaaaataa
     7801 aaccaagact tatatacatt atgcctaatt ttcaaaatcc aactggaata tcttactcaa
     7861 catataaaaa aaagaaattg atagaattgg cagaagaaca tgatttttat ataatagaag
     7921 atgattttat aagtgatttt gtttttgaat caaaggacaa tagaacaatt agaagttatg
     7981 atgataaaaa tagagtagta tatataaaaa gcttttcaaa aatacttatg ccaggtctta
     8041 ggataggaat tgtagaaatg ccaaatgagt tgttaaaaaa agttttatgg gctaaatatt
     8101 catcagatat atcaacacca ggtttaattc aaaaatcaat gtattactat atgaataatt
     8161 ttgattggaa gaattatttg agtcatatag aaaagatata tactgataag tataaattgg
     8221 caaaaaactt gattaatgac aaattgagtg gtaaattaaa agttagaaaa tcttgtggag
     8281 gaataaactt ttttttagaa ttaccaagag gatatacatc tcaagcattt acaaatttta
     8341 tgttaaacaa aggagtgtca atgcttccag ggacttattt ttttgacaat ctagtggatg
     8401 atagattctt taggataaat atagctcgcc caagcatgga agaactagaa aagggaatat
     8461 ctataataag tgataatata gatgagtttt tttgtgaata taaaaacaag ttagatataa
     8521 aaagcaataa attattttat taaataaata gaggtttttt aggaggaaaa aatgtttgac
     8581 aagaagaaat tagataggat taatgaatta gcaaagaaaa ataaagaggg aatcctttct
     8641 gctgatgaaa tcaaagaaag agaaatttta aggaaagaat atttagaaaa ctttagagcc
     8701 cactttagat caagactaga tagtgtcaaa gtagtttcac cagaagaata tgagcaatac
     8761 atgaaaaaca ataaaaatta gaacttagta ggaggaaaat atgaacttaa agtacgaatt
     8821 taagaatgct tgggattttg aaagaaaaga aaatactatt gaacaaatta tgcaatattc
     8881 tagcaattat atggagtttt taagtaagtc aaagactgaa agactttcag taaaagaaat
     8941 tataaaattg gctaaagaaa ataactatat ttcaatagat gaagctatgg aaaaaggaag
     9001 cataagttgt ggtgataaga tttatgttat aaataaagag aaggcagtag cactttttgt
     9061 aataggtaaa aattatatag aaaaaggtat gaagattata ggaagccata tagattcgcc
     9121 aagattagat ttaaaaccaa atccactgta tcaagagagt aatctaggtt tttttaagac
     9181 tcattattat ggtggaataa aaaaatacca atggacggct attccacttg ctttacatgg
     9241 tatagttata ttaaatgatg gaacaaaagt ggacatatct attggggaag aagatagtga
     9301 cccagtattt tgtgtaactg atttattgat tcatttggct ggagaccaaa tgcaaaagaa
     9361 actatcagaa ggaattagtg gagaagcttt aaatatatta attggaaata tgcctttaga
     9421 agatgaagaa aaagaaccta taacagcaaa tatattaaag attttaaatg agaaatacaa
     9481 tatagttgaa gaagatttat taagtgctga aattgaagta gttccagctg gtaaggctag
     9541 agatttaggg cttgataggt ctatggtgtt gggatatggg catgatgata gggtatgttc
     9601 atatgctgct gtaaaagcta tactagaaac tgaacaacca gaatttactt cagttgcatt
     9661 atgtgtagat aaagaagaaa ttggttctaa agggaataca ggtatgcact caaagttttt
     9721 tgaaaatact gtggctgaac tgattgcttt agaaggagat tattgtgata ttaaagtaag
     9781 aagagcttta gcaaatagta aggtattatc agctgatgtt tctgctggat atgacccaaa
     9841 ttttggagaa gcatatgaaa agagaaattc ggcatatatg ggaaatggag tagttttaac
     9901 taagtataca ggttcaagag gaaagagtgg atgtaatgat gctaatgctg aatttatgtc
     9961 agaagtaaag agaattttta ataaaggaaa tgtaatatgg caaactgctg aacttggaaa
    10021 agtagaccaa ggaggaggcg gaactatagc ccatatattg gcaaatcaag gtgcagaggt
    10081 tatagactgt ggtgttggag ttttaaatat gcatgcacca catgagattg tctctaaagt
    10141 agacatatat gaaatgtata agggatataa ggcatttttt aatataaatt tataagtaaa
    10201 aatataaatg taataagtga ataaaggctg ttgtataaac aatttaatct gttgtacaac
    10261 agtcttattt ttatgtaaaa atagcaattt gtcacactag agaaaataaa agaaaatact
    10321 gtcaaattaa aaattattta aaaaaaaatt gacaacgatt tcatcttgtt ataatatgta
    10381 ataaagaact tgttatataa tgttataaaa atgttacata gggaggtgag caaaatggat
    10441 gaaaagagac aaaaaaaaga aatggattta gaagtaaatt cctttgttcc tttatatcaa
    10501 caactatatg acaacataaa aaaacaaata gcatctggca tatataaacc aggagataaa
    10561 cttccatctg aaggagactt atgtaaagaa tttaatataa gtcgtataac tgtgagaaat
    10621 gcacttaatg aacttgtaaa agaagatata ttatgtaaaa aacgtggtaa agggacttat
    10681 gtaacaatac cagagagaat agaagcaaca tgtgcaggaa atagttttac taattcttgt
    10741 catcgtatta atgcaaaacc aagtaccaaa ataatctctg ttttaataaa aaaagcagat
    10801 aagcaagtag ctgaagcttt aagtattgaa ctagaagaga aggtaatatg tattaaaaga
    10861 cttagattaa tagatgaagt acctgttatt tttgaagttg actattttag aatagattac
    10921 atgtttttat taaaagaaga attagaagat aaatctttaa tggaggttat atctaataat
    10981 ataagcacat tacctaaacg agtagaaaat atatttgaag taaaacattc aaacaaagaa
    11041 tattctgacc atcttaaatg tgcaagtaat atgccactat tgaaagtaag acaatcggtg
    11101 tatacagaaa ataatgatgt cttatattac aatgagcaat ttataagaag tgataagtac
    11161 aaatatgcag tatcagcaga aatataatct ataatttagt aggaggatga gtatgaaaaa
    11221 gataatatta gcatctcatg gagacttttc aaaagggctt ttaaatgcag taaaaatgat
    11281 agtaggagat ttagcagatt gtgtcagttc gtatggtttg taccctggac aaagtgcttc
    11341 agattttgct ttagaattag aaaaaacaat atcagaagat aaagaatgtg aatatataat
    11401 tttatctgac ttatatggag caagtgtatg tacagcaatg ttaagattaa ctaatctaca
    11461 taatgttagg ttattttctg gaatgaattt gaatatggta ttagaacttc ttacaaggtt
    11521 ttctgatgaa ttaacagttg aagatataga ccaattagtg gaagaatcta gaagaggaat
    11581 acaaagcgtg acattacaaa ttaaagaaga agaagaggta ttttagttta tgaaaaaaaa
    11641 gggaatatta tcagtattta aaagtaaaaa accaattata gcaatgatac acttaaaagg
    11701 agatactcca gaagatatct ttgaaagagc taaaaaagag attacaatct ttgaagaaaa
    11761 tggtgtagat ggaataatgt tagaaaatta ctatggtaat tattatgatt tagaaagaat
    11821 acttgaatat gtatcaaaag ctaatttatc aataccatat ggagtaaact gtttaaatgt
    11881 tgatactatg ggatttgaac tggcaacaaa atataatgct agttatatac aggttgattc
    11941 tgtagtaggt catgttaaac ctagagatga agctacttta gaagagtttt ttaaattaca
    12001 acgttcaaaa tgtccagcgt atttaattgg aggcgtacgt tttaaatatc agccagtatt
    12061 atcagaaaat gatgttgaag aagatttaaa aataggaatg acaagatgtg atgctatagc
    12121 agttacagaa aatgctacag gtcaagaaac atcaatggaa aaaatagaac tgtttcgtaa
    12181 aaatttagga gacttcccat tggttatagc tgctggagtt acattagaaa atgcaaaaaa
    12241 acagcttaaa ttaggagata tggcaataat aggaagttac tttaaagata attacaaaga
    12301 ttttggggat gttagtgtag aacacgtaaa aacattcatg gatgaaatca aaaaaattag
    12361 ggaggaatta taatgattaa attagtaaga gtagaccaca gacttataca tggacaagta
    12421 gcatttacat ggacaaagtt tttaagtaca gactgtatat tgatagcaag tgatgactta
    12481 ttaaaagatg aattaagaat ggcaggactt aaaatggcta aaccatctaa tgttaagtta
    12541 gtaatgaaaa gcatagcaga ttctataaaa gcacttaact caggtgttac tgataaatac
    12601 aacttattga tactttgtga atctgtagaa gatgtttaca gacttgctaa agaggtaaaa
    12661 gctattaaat ctataaacct tggtggaaca aaatcagatg ataatcgtga aaatatatct
    12721 aaagcagtgc atgtatcaaa agatgatata aagatgatta aagagttaga ttcagaaggc
    12781 gtaaatgtat ttgtacaatt agttcctgat gatgatgcta caaatgtaat gaaattaata
    12841 taattttagg gggatgaaaa tggaatttac acaagttatt ttgataactc taattgcatt
    12901 ctttgcatat atgcatagtt ttgtaggttc tacaatgcat aatagaccta tagttgttgc
    12961 accacttgtt ggtttagcac taggtaatct tcatacaggt atagtaatag gttcaacatt
    13021 agaattagta tttatgggtg catttcctgt tggggcaagt aatcctcctg attttgtgtc
    13081 tggttctatt attgccacag cgtttgtaat attaacgggt caagatgtat cagcagcagt
    13141 tgtacttgca gttcctatag caactctagt attgcttata gataacttct taatgacagt
    13201 agtacttaca tggggagctc atattgctga ccgttatgca gaagagggga atattgaagg
    13261 cgtagaacgt gtgcaattat tatttggtat aggaaataaa ttaatattag caataatagt
    13321 aggtatagga ttttcattag gtgttccagt aatagaaaaa atactttcat ttataccatc
    13381 atatgtaact catggaatgg atgttgcagc aggcgtgatt ccagcaatag gttttgccat
    13441 gttagctaga atgatgctta ataagaaaac agtagcattt ttattattag gatttatatt
    13501 ggttgcttac ctaaacataa cagtaacagg agttgcattg tttggattag caatagcttt
    13561 aatatatgtt aactttgtag gagagaagga ggtaatagta gatgacaacg agttctaaga
    13621 aattagagac aatttcacca gatagtaaaa taacgaggaa agacttttgg aaatgtttta
    13681 gaaggtcatt gacattagat tcatcttgga actacgaacg tatgcaaaat atagcatatg
    13741 catacatgat ggcaccaata atccgtagat tatataaaga tgataaagaa aagaaatcta
    13801 aggctttaaa aagacattta gaatttatgt cagttactcc tcatataagt actcttttgg
    13861 taggtatatc tggagctatg gaagaagaaa atgcaaagaa taaagaattt gatgctaata
    13921 gtataaatgc tgttaaatca agtttaatgg gtccagtttc tgggatagga gattcatttt
    13981 tctggggaac attaaagctt atagcagctg gggttggtat agctttagct tctcagggta
    14041 acataatggg accgatttta ttccttttaa taataaatgt tcctcatttt attatacgct
    14101 atatatgtct agataaagga tttaagtatg gaactcaatt ctttaaagat gtgagtggtt
    14161 caagtatagt ttcaaaagtt atggaagcag cttcaatgtt agggcttatg gtaattggag
    14221 gtatgactgc atctaatgta atgttaaaac ttagtgttaa tgtaggtagt ggagaatggg
    14281 cagagcctat tcaaacttac ttagaccaaa taatgccatg tatgcttcca gctatgatat
    14341 tcggtattat gtattggtta ttaggaaaaa aggtaaagac aactacgata ttaatttcag
    14401 ttatgattat ttgtatagta ttggctgcta ttggagtagt ataaataaaa aaatcccaag
    14461 ttcatttgaa cgagggattt tttgactaaa ttaaataaaa aagaaagatg gttcataata
    14521 atttataagc atttatgtga tagttataat tagcttatta attagttata aactcaaaat
    14581 taagcactac tttattaaag ttattatatt ataatttcga aatactcaca taatctgttt
    14641 agaaatttat cttctaatgg ggaaaaagtt ttctttttag agtaaatgaa gaaaaacttt
    14701 ctagtaaaat caacatcctt tattttataa cattttattt tacctgcatt gagataatcg
    14761 atagctgaaa tgtacgatat aaaggacact ccaagaccta atctaaccat ttccttaata
    14821 gcctcatttg attctacatg agcgattaca tttaatttac ttactggaaa gttattttta
    14881 ctaagtgtgt ttagtattaa gtttcttgta cctgaccctt cttttctcat aataaaattt
    14941 agataagcca attctcctat atcgatacaa ttatttttat tatcaatttt aaaatctgaa
    15001 ggagtgataa gaacaagttc atcatctaat aagtctaggt actctatttg aggattattg
    15061 attttagaac caactaatcc aaagcttatt cgttcattta aaatttctga tatagcatat
    15121 tgagaatcat aatggcttat ggaaaatttt acatctggat aactcataga aaaactcttc
    15181 ataaaatcag gtaaaatata agtttcaggt atggaactac atgctatatc tataatacct
    15241 tctatttttc ctgaatattc ttttatatca taaattgcac gtttgcaatt attcaatata
    15301 tatatagcat gttcataaag aatttcacca gattttgtga gtgtaataac tttgttgctt
    15361 ctatttataa gaacagtttc tagctctctc tctaaattct gtatgtgtgc gcttacagta
    15421 ggttgagtta aaaaaagttc tcttgcagcc tttgaaaaac tctgatgttt tgcaacagca
    15481 acaaatactt caagttgttt aaaatccatt atttaatcct ccttaaatta tctaaaacta
    15541 ttataacata tttgataaat taatagtcaa aggtgatttt taaaatagaa aacctttaaa
    15601 tattcatgga gtttttgatt gtttaggaat aatttggtat aatgttgtaa ggtacttaca
    15661 ataaaaatat cgtaaattga attttcaaaa ttcaacttta aagtgttttt tattacagaa
    15721 aaattacaat tagcttctta atcttattaa attcaaataa ata
//
LOCUS       DAYATN010000090        15570 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.090, whole genome shotgun sequence.
ACCESSION   DAYATN010000090 DAYATN010000000
VERSION     DAYATN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.090"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..569)
                     /locus_tag="ACGI8A_002562"
     CDS             complement(<1..569)
                     /locus_tag="ACGI8A_002562"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180553.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIG"
     gene            739..2097
                     /locus_tag="ACGI8A_002563"
     CDS             739..2097
                     /locus_tag="ACGI8A_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180554.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            2090..2722
                     /locus_tag="ACGI8A_002564"
     CDS             2090..2722
                     /locus_tag="ACGI8A_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFH0180555.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            3051..4208
                     /locus_tag="ACGI8A_002565"
     CDS             3051..4208
                     /locus_tag="ACGI8A_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFH0180556.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(4355..4990)
                     /gene="lexA"
                     /locus_tag="ACGI8A_002566"
     CDS             complement(4355..4990)
                     /gene="lexA"
                     /locus_tag="ACGI8A_002566"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFH0180557.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(5094..6221)
                     /locus_tag="ACGI8A_002567"
     CDS             complement(5094..6221)
                     /locus_tag="ACGI8A_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0180558.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(6532..7377)
                     /locus_tag="ACGI8A_002568"
     CDS             complement(6532..7377)
                     /locus_tag="ACGI8A_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180559.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(7407..7778)
                     /locus_tag="ACGI8A_002569"
     CDS             complement(7407..7778)
                     /locus_tag="ACGI8A_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFH0180560.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(7931..8191)
                     /locus_tag="ACGI8A_002570"
     CDS             complement(7931..8191)
                     /locus_tag="ACGI8A_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HFH0180561.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(8380..9324)
                     /locus_tag="ACGI8A_002571"
     CDS             complement(8380..9324)
                     /locus_tag="ACGI8A_002571"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFH0180562.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVVGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(9345..10199)
                     /gene="accD"
                     /locus_tag="ACGI8A_002572"
     CDS             complement(9345..10199)
                     /gene="accD"
                     /locus_tag="ACGI8A_002572"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFH0180563.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(10214..11575)
                     /gene="accC"
                     /locus_tag="ACGI8A_002573"
     CDS             complement(10214..11575)
                     /gene="accC"
                     /locus_tag="ACGI8A_002573"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFH0180564.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(11591..12043)
                     /gene="accB"
                     /locus_tag="ACGI8A_002574"
     CDS             complement(11591..12043)
                     /gene="accB"
                     /locus_tag="ACGI8A_002574"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFH0180565.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(12313..12759)
                     /locus_tag="ACGI8A_002575"
     CDS             complement(12313..12759)
                     /locus_tag="ACGI8A_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180566.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            13034..13312
                     /locus_tag="ACGI8A_002576"
     CDS             13034..13312
                     /locus_tag="ACGI8A_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180567.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     ILVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(13441..14172)
                     /locus_tag="ACGI8A_002577"
     CDS             complement(13441..14172)
                     /locus_tag="ACGI8A_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180568.1"
                     /translation="MNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDANLRAVV
                     KRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEVPKEAL
                     SLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHNVDIAK
                     GESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGNKTGKA
                     VVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(14316..14960)
                     /gene="trmB"
                     /locus_tag="ACGI8A_002578"
     CDS             complement(14316..14960)
                     /gene="trmB"
                     /locus_tag="ACGI8A_002578"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFH0180569.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(15098..15418)
                     /locus_tag="ACGI8A_002579"
     CDS             complement(15098..15418)
                     /locus_tag="ACGI8A_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HFH0180570.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT         5464 a         2371 c         1860 g         5875 t
ORIGIN      
        1 ccaatatcat gtgtaaccaa aatagtagac ttattctcat tcttaataat actataaaca
       61 tcatcacaaa ctaataatct agtctgataa tcaagagctg aaaaaggttc atctaaaagt
      121 agaatatctg gatttacaga aagtgttcta ataagcgcaa ctctttgtct catacctcca
      181 gataattctt ttggatacat acttctaaaa tcccataatc cataagtctt taaaaggctt
      241 tcaactctat ctaaagattt tttatctttt ttgccttgaa tttccaatcc aatagttata
      301 ttatccatta tatttctcca ttcaagcaaa ttatcttttt gaaacatata tccaatattt
      361 ccagtaatct ttatatcacc actagtcggt tttaaaagat tagtaagaat atttaatatt
      421 gtagatttac cacttccaga aggaccaagc aaagttaata tttctccttc tttcaagtta
      481 aaattgacat cctttaatac ttcaagctcg ccttccttag tgtagaagtt catatttata
      541 tttttaactt ctactatgtt gtcattcatt atgccacccc ctataacttt tagctcgtat
      601 atattatgtt attaacaata tgacctattt gtaccatttt tcataaatat aatataaata
      661 ttttgtttat ttacgtattt tcttagtaac ttgtacgtat aaattataga taaataaatt
      721 ataaggtagg ggataaattt gagtaacata ataacgaatt taatagagtc aatatttact
      781 aatccaataa ctatattgtt tttacttgca ataactgtgt attctgtttt taaaataata
      841 gaaaataaca gagtatattc atttgtaagt tcaaaattag acgaaatcaa tagagactat
      901 aagacaagtg agttttataa aaaggtaagg gatgactact atacatatag taaagaaaat
      961 ccttatgcag acgtaaatat aacttcattt atagaggaag ttgtatctga tttaaagcat
     1021 aataatttgc cattgttaga aaaaattaga agtataaaaa attcatcttc aataagtata
     1081 ttacttgggg ttttaggtac gtttgttggt ctttctacaa tgcttttatg tgttgatact
     1141 aaagacatta taaattcact tccttcaact attagtagta tgcaaactgc atttacaact
     1201 agtatatttg gagttgtatt ttctctaatt ataggttact ttactaagat aaaagattgt
     1261 gaacatgttt tgatacaaat aatgttaaaa tctgaaaatt tactaacatc tgaaataact
     1321 cattttaagt cagaaagaat ggatttaaaa gtagaagagg taaaaaatac aataaaacaa
     1381 ataagtaagt ctatagaagc tattgaaaaa tttgataaaa tatctaaaga tttaaatgac
     1441 tttaatgatg aatttatcag tggtatagaa gctttaaaaa gccttttaga aggttctcaa
     1501 agttctatta agacatttga ccagagcgta agaaaattag ataaacagtt taatatttta
     1561 aatataaaat ttgtaaagct ttttgataaa tatgataatc aagataatat aaataaagag
     1621 attttatttg atataaaaga atcttcaaaa aatatataca atgccactga aagccaattc
     1681 aaaattagag attatataaa aaatataaat gcaggatttg ctctatatga aagaagtgct
     1741 caagatttat taactaaatt aatgacccat gaaaataaaa tatctcaaaa tcaaaaaatc
     1801 cttttagatg aaaagtttac cttagatgac tcaataaaaa atctctcaag tattattgaa
     1861 aacttttcta atgacctaca agttaaactg gatatgatgt ttgaaaattc tttagatatt
     1921 caagataagt tagatgttat gtttaataac tcttttatga atgatgaagt tcctcttgat
     1981 agtgaagaat tatttaagga tgatgttaat aatgtattta atccttttag tgaagaaatt
     2041 tatgagatag aggataaaga aataaaggtc ataggtgagg atgagttaaa tgaataataa
     2101 atatagaaga actgttaaca gagaatttga aaagagtagc ttttggccaa catttacaga
     2161 tttattatct actgtattga tggttgtgat tcttatctta tttagctcag aaagtatttc
     2221 aggttctgtt gaacaagatt tagctaagaa tgtcaatgct tctgttgaag aaacttttaa
     2281 aaaaagtgga atacctgtga aggtagataa atctaatggg caagtaactt ttggtgaaaa
     2341 aaccatgttt gatgtagata gtgatgtttt aaaaccagag gctaaagaaa tgctaaaaat
     2401 gtttgttcca aagtacatag agactattta taaagattat ggtgactata tatccaaaat
     2461 agttatagaa ggtcatacag atgatgtggg aagttatata tacaacttag atttatcgca
     2521 aagaagagca tatagcgttg ctaaatttat agtgggagat gaaataggtg actataaata
     2581 caaagataaa gtgactaagc atattattgc aataggaagg tcaaaagctg aactcatcaa
     2641 aaatggtgac aatagcgtga atagagatgc atctagaaga gtagaactaa aatacgaaat
     2701 aaatattaat caaaataaat aattaaattt ttataaaaaa aaagaaggca gttggtgtga
     2761 gctttaagcc ttctttaagg aaaaagttta tttatttttg ttgatactta acatattata
     2821 gtttttagaa gcagtttgca atatattaaa cgtaaatggt cgtttaaaat gcataactgg
     2881 atactactga tgtaaaatat ttcattttat ataatataaa aattatacag tgtcaatttt
     2941 ctatattatt gtactataat ctattattta aaatattatg taaaaaaata tcataaaaac
     3001 gcatatcatg ttataattac tagagtatta tgaatatgga ggcgtagttt gtgatagaaa
     3061 taagtataaa aaataaaaaa aattctaaaa ttaaaacttt gatactttta ttgatagtat
     3121 ctatatcgat ttatttaact ggttgtacta catcagataa tggaaataat caatcaaata
     3181 ataatgattc tttgtctagt agtatatcaa gtgataagaa tgtaaaaatt cattttattg
     3241 acactggaaa tagtgatgca atattgattc aagatggtaa aacttttaca ttgatagatg
     3301 ggggagataa tgatgatgag ggattaatgg tagattacct aaacaatcaa ggtgtaaaag
     3361 atattaaata tcttatagct acccattctc atgcagacca tttaggtgga cttgattctg
     3421 ttgtcaaaaa ctttaatata gaaaatgttt ttgtatcgaa tggttcagca gaaacaaaga
     3481 gttataggga ctttataaat gctttagcaa ataagggttt atccccaagt gtaccgcttg
     3541 aaaataataa gttctattta gaagattctt attttgaagt tctaaataca aatgggggag
     3601 atactactaa tgaacaatct ctagtccttg tatatactaa tgggaatgat aaagttttat
     3661 ttacaggtga tgctgaagaa ggtaccgaaa aagaaatact acctaagctt gagaaagtag
     3721 accttttaaa agtagctcat catggttctc gtagtagttc atctcaagag tttttagata
     3781 aagtaaatcc tgaatatgcg gttcttttag taggcaaggg taattcatat ggtcatccac
     3841 atcaggagac aatgaataaa ttaaaaaaaa tgggagtaaa agtgcataga agtgatgagt
     3901 gttcagatat aatatttgaa tcaacaggtg atggagtatt tacaagttgt aaagatggaa
     3961 gttataataa aggcgttaga gaagatggaa actctggaaa atcaaacagt aatacaagta
     4021 aaaatgattc ttttaaaaat aaacaaaata catccaattc agaagaaata gtgtatttta
     4081 ctccaaaagg taagagctat cactctacaa aaaattgtag tgggctttca agaagtaaaa
     4141 aaatattatc aggcacaatt gcagaaagta agaaaaatga cccatgtgat atatgttatg
     4201 gaaaataaca aaaaaacaac caatttatct ataaagcttc tgaactttaa atcatttaaa
     4261 aaataaaata aatgcataaa aaaataagct ataatcaata gataacacat tttatctata
     4321 cgaatagctt atttttttaa gtatttttta atagctattt aataactcta aatacaccag
     4381 ttacaatacc tacaatttca acttctgagt cattgagcat aattggttcc ataaaatcat
     4441 tttctggctg aagtcttatt aaatttcctt ctttaaaaaa cctcttaacc gtagcagatt
     4501 caccatttat aagtgcaact actatctgag aatttaaggc tgtatttttt ttgtctacta
     4561 caacgtaatc ttcatctaaa attcctgcat taatcatact ttcgcctttt actcttaata
     4621 caaaattgtt actgcccttt actaaactgg cagggaatgg aatgtattcc tctatgtttt
     4681 gttgagctaa aatgggttct ccagctgtta tttgacctac taaaggaagc tctattattt
     4741 cttgattagc tccagaaaca tcatttactt tactacgttc taaaacttca atagctcttg
     4801 gtttagtagg gtcctttctt atgtatccca gtttttcaag tttatttaga tgagagtgca
     4861 cagttgaagt agatcttaac cctacagcag tacatatttc tcttacagca ggtggataac
     4921 cttttaatat gatttgagac tttataaatt ccaatatcaa gacttgcttt tcagttagat
     4981 ctaaatacat aacatcactc atttccaaac atattttgtt tttatttcca tacactttat
     5041 atttttaatt ataacataat ccatttatat ctgcaaacat ttgttcctga tttttaatcg
     5101 tcctcaatcc attcaaaatt cttgactata tcacgtttta catttttctt atgagctgca
     5161 taaagctgag tagttgtgac attatcatgg tcaagtaagt tttgaacatc atatatagaa
     5221 aatcctgttt ggattagaga agttgcagcc gttgctctta atttatgggg actatatcca
     5281 ttgtctctag tagtatcaag tggaatagat gtatattttt ttaccagtgt tcttatagcc
     5341 tttgctgtaa ttctcttatt ttgaagagac aaaaataaag catctttttc ttcatcattt
     5401 aatagttcat ctcttgttct ttcgttttgc aaataatctt ttattacatg ttcacaagta
     5461 tgatttatag gcattaaaac ttctttccca cgttttctat atattttaaa ttcacctctt
     5521 gaaaaattaa aagaggagat attgagttct ctaagctcat ttaatctaag cccgtatgta
     5581 acaaataatg ctagtatagc tttgtcacgt aatttagttt ttctccaata taccttttct
     5641 ttctctgtaa gcccttcacc tgtttctact gattcaagca tcttagcaac ttcatctatc
     5701 tcaaggcgct taatagcatc tggctgaggt ttaggaagtt ttattggatt gaatccatca
     5761 gttatattat tatctatctg agagtttctg tataagaatt taaatagagt agagatggat
     5821 gatttttttc ttgctaaagc tctattgtta ttttcaaaaa tcaatgtgtt tttttctgta
     5881 tttttgtaat atctactgca ataatcacct aagaataaat ttatatctct tgattttatt
     5941 gtattaaaat catcttctgt aatgtctttt acacaatctg catttgttaa ttctttggtt
     6001 tcaatcaaat atgaacaaaa aaaattaata tcttctaaat aagcaagtcg tgtagaaact
     6061 gcaacagaac cttttaaata tataaaataa tccttcataa atacaggaag tttattttcc
     6121 aaaagtagac atttttctat gagttcattt tctttgataa caatattatc aggtttatct
     6181 gatttattat gtacttttat aattttctta tcattattca aaattaaacc tcctaaaact
     6241 aaaatataaa tcttttatat tattttttga tatttatact aagtgagtat aaatatttaa
     6301 tttaatcatt ttttacattt ttaagagatt agcagtgctt tagatgaaat tatcactaac
     6361 tattccctaa atccacattt aaagactagt tgaatttaag tactcttttt tatttctcaa
     6421 tatatttata taaatatatt gagaaatttt ttatgctcta ctagaatctt ttttatataa
     6481 tatttttata tgtttctaac gaaaccaaat attagtaaaa tatgtattta tttaactaaa
     6541 gtttttaaat gggctgacta agcttaaaat ataggatatt atgagagtta ctattgaaaa
     6601 aataaaccct attgccataa agaaacttat aaatatgtta tacttaacta ttaaactgga
     6661 tatattcaag ttatcaaatc caaataagta tgtaattgaa cttattacaa aaataactat
     6721 aactataaag ctcaggccat ttaagctatt ttttaaatca gcactactaa gtgacatatg
     6781 tgtagatata gaatacatta aaaacataaa tataaaatac ttcaatgaat atacactagg
     6841 tgttaacaac attattctta taaaattgga aatatcacct aaaataatgt gtataaaacc
     6901 atttatattt aaattattaa tcatgcttac atataacttt aaatctatat tttgagtcac
     6961 tttcatataa ctatctggca gtaatatctt aaaaattaaa ataatcatta ttgttccacc
     7021 tataataggt gcaattccta taaagaaatt tcctatactt tgataaagat tatctttttt
     7081 atagctatga cttacatatc ctagtatacc atcattttta ctttctattg gtctgaaaaa
     7141 cttaactgaa tttattttat gcatgaaaat taaacacatt atcaaatgac tcaattcatg
     7201 aacaaatgtt cctatagctc cagttacaat aattccagtt tttccaaaag atgaaaatat
     7261 aaatttatta tttagatttt ctgtaatgct aaaaatcaat ccaaatacaa taaatattcc
     7321 tatgaaacaa aaaatattta ttacacttat ttttaaaatt tcgattatat aaatcataag
     7381 ttaaatcctc atgtaaaagt atttaactac tctacttcat atccattaca gcttaaaatt
     7441 ttacaagcat tttctatatc cttttctttt actaagatat agtctgtatc ataagtagaa
     7501 attgcaaata tgcttatttt gttgtctgct aacaaactac ttaacttaga taaaattcct
     7561 acaagtgaaa agtctaaagg acctagtatc ttaagtattc tccactcctt ttctgactta
     7621 acatcagaag gaatattatt atttaaacat acaacactta attcatcatc agttctagtt
     7681 atagagtaaa acttctctgt atctatccat gttggtattt tactgtcatt atttaatcta
     7741 catactgcat actcttctac caataatttt aatttcataa tgtatatttc ctccaatctt
     7801 atttgtttac ttaagattat acattaaaag ttacataaaa taaaacatat aaagtaaaaa
     7861 aggttcacta tagtgtccta tttctatagc caacctttaa aacttaatta aattctaata
     7921 aagagatata ttatctaggt tcaattataa gtttaatggc tgttttctta tcaccttcga
     7981 tttctatatc agtaaatgcg ggaatgcaaa ctaaatccat tccactaggt gcaacaaaac
     8041 ctcttgctat tgcaattgat tttatcgctt gatttagagc tccagctcct atagcttgta
     8101 tttctgcaac gcctcgttct cttagtactc cagccaaggc tccagctaca gaattaggat
     8161 tagattttga tgaaactttt agtacctcca tttcaacatc tccttgttaa tttgttaaag
     8221 tatccattac ctaaagtact aaatcataat tgccatttac taattacaat catcatttaa
     8281 agtatacttt tataaaaata tacttctata atcgttttaa gaaaaccttt atttttttta
     8341 accattttat tatgaaaaaa ctacaaattt ctttatattc tataagcatt ttccaatatt
     8401 tctaaatttt ttatatcttt gagctataat ttcctcttta tccatatctt tgtaatgatt
     8461 taatctttct aaaatgtaag cttttatttc ttttgaaaca gcttctacat ctttttgtgc
     8521 tcctccaaga ggttctttta taatcttatc tattattttt aaatcatata aatcattact
     8581 tgtaagcttc ataacatcac aagcttcttt agctttagat gaatctttaa atagaatact
     8641 agaaagccct tctggagaaa tcacagaata aactgaatgt tctaacatac atacatcatt
     8701 tcctactccg atacctaaag ctcctccact accaccttca ccaactacaa aagtgattac
     8761 tggaacagaa agtttactca tttcaagtaa gtttcttgca atagcttctc cttgtcctct
     8821 ttcttctgct tctagaccac aaaaagctcc tgatgtatca ataaacgtta ttataggtct
     8881 attaaatttt tcagcttgtt tcataagtct taatgctttt ctgtatcctt ctggatgagg
     8941 cattccaaaa tttcttttta tattttcttt tgtatctctt cctttttggt gtccaactat
     9001 agtaacatta aactttccta tctgaccaat tcctccaacc atagaagggt catctttaaa
     9061 aagcctatct ccatgaagtt ctaaaaaatt agaacaaata tgttctatgt attccaatgt
     9121 cgtaggtctc tttatatctc tacttaatgt taccttttca tatgctgata gatgtgaaaa
     9181 agcatcttcc ttaagctttt ccaatttctc attaagtgaa tttattttat cacttaaatc
     9241 tatattattt gccttagaaa tagttattaa ttgtttaata tcattttcta agacttttat
     9301 tttttcacta ttattttcaa tcaaaacagt tcctccttta tgccctattt cttgtgcata
     9361 gcaagaattt gatataatac ttccttcatt tttcttctat ctactatcat gtcaataaaa
     9421 cctttttcaa gtaaaaactc agaagtttga aatccttcag gaagtttttg atttattgtt
     9481 tgctctatta ctcttggacc tgcaaaacct atcaacgtat ttggctcaga aattataata
     9541 tcaccaagca tagcaaaact agcagttact cctccagtag tagggtcagt tagaactgat
     9601 atatatggca atgatttttc ttcaagctta gaaagtgctt gagaagtctt tgccatctgc
     9661 ataagagata ccataccttc ttgcattcta gctcctcctg aagcagaaca aataattata
     9721 ggtaaattat tttcagcagc atactctatt gaataagtta ttttttcccc aacaatagaa
     9781 cccatacttc ccatcataaa atcaggattc attacacaaa taactgtttt aattccattt
     9841 attcttccat aacctgtgac aacagcttct gactcttttg ttttttcctt ataagagtct
     9901 actttttctt tgtatttagg aaaatttagt ggattttgaa attctacatc agaattaaac
     9961 tccataaaag ttcctttatc aatcaataaa tttattcttt ttcttgctct taagccataa
    10021 tgtcttgaac attttggaca tacaaatgaa ttttcttcta tgtcctttct aaatacatga
    10081 ctatcacaac ctttgcaata ggtccaaaat ttatcatcta cactattctt tttaaagttg
    10141 tcatctaaag aaatcgtaac atacttagat tcttttgatg ataaaaattt ttttatcata
    10201 atctatttac tccttataat gtagcaaatt ctttcttcat aaagacatct tctataaatt
    10261 tggtatgatg agtatcttct ctaaactcct cacttgttac aagtgccttt tgaaattcta
    10321 catttgtatt aataccttct acaataattt cgtctagagc tctatatatt ctacttatac
    10381 attcttctct agtttcagcc caacatatta acttacctat catagaatca tataaaggtg
    10441 gtatagtata tcctgcatat acaaaagtat caaatctaac accaaaacct ccaggtaaat
    10501 ttaatgattc tatcttacct ggtgatggag caaaattctt tgatgaatct tctgcattta
    10561 ttctacattc aatagcatga cctctaaaag taatatcttc ttgtttaaaa ggaattttat
    10621 ccccattagc tatagatatt tgaagtttta taaggtcaag atttgttacc atctctgtaa
    10681 ctggatgttc aacttgtatt cttgtattca tctctataaa ataaaagttt ttatctttgt
    10741 caactataaa ctcaatagta cctgcaccga tataacctac tccttttact atatctactg
    10801 caattttatg aagtttttgt ctttcttcat ctgaaaggta tggacttaaa gattcttcta
    10861 ttactttttg atttcgcctt tgcatagaac aatctctatc acctaaatgt attgcatttg
    10921 aatgtttatc tccaaatact tgtacttcta tatgtctagg attttctata aacttttcca
    10981 tatataaatc actattagaa aatgctgcta gagcttctga acttgccata tcaaaatttg
    11041 aaaaaagttc ttcttctttt ctaacgattc tcataccttt tccaccgcct ccacttgagg
    11101 cttttatcat aacaggatat ccaatttctc ttgcaacttc taaagcttct tccttagttt
    11161 tcaaaacaga ttctgaacca ggaacaacag ggatattcaa ttccatcata gtttctcttg
    11221 ctctagattt atctcccatc aaaatcattt gttctcttgt tggacctata aaaataatat
    11281 tattatcttc acattgcttt gcaaattcag gattctctga taaaaatcca aagcctggat
    11341 gaatagcatc acactttaat ttaagagcaa gatagattat attttctata ttattataac
    11401 tcttacttat attatttggt cctatacaaa tagattcatc agctatatat ctatgaaaac
    11461 aatctttatc tatttcagaa tagatagcaa cagtttttat acctagttct ttacatgttc
    11521 tgatgattct aactgctata tcgcctctat ttgcaactag tatttttttt atcaacttaa
    11581 atacctccta ctacaatggt tttatcttaa ataaaggttg attatattct actagttctt
    11641 cattactaac taatacttca attatttcac ctttaatttc acttgttatc tcattcataa
    11701 gcttcattgc ttctaatata cataaagtgt cgccttcttc aactacatcg ccaactttaa
    11761 caaagctatc tgcatctgga cttggagaat catagaatgt tcccatcaaa ggagctacta
    11821 ctactgatag attctcatta gatgtgacag gtgtatcgtt tacaactggc ttttctaaag
    11881 aaagatccac tactgattct tgttgtacac ttaaaacagg tgaacttgta gattgagctt
    11941 ttttagatac ttctaatctt aaatcgctac tttcaagttt tacatattct aagttagttg
    12001 agtctatggc ctttaataat tccttaattt cattgatgtt cattttgtta cccccttgta
    12061 ttattacctg gtgctaatat tatatcacaa aaaacaaatt tataaaagaa aaagtgctga
    12121 ctagagaatc aacacatcaa tatatctttt aattttaaat taacaaccag taacttatat
    12181 ttgttttaat tttccattat taatataaat tataagtata aaatgaataa aattcaatat
    12241 tttttgcaaa atacaagaga atacaaatta atacctcggg aatttttaag acaaatacca
    12301 atattatgca atttagactt cgctttcttt caaattagta tcattttctt tatttttaca
    12361 taggatgtac tttaatttag ctatcaaaac attgctatat aggttaaaag ttattagaat
    12421 tgcaggaatt ataaagtaaa ctctagcacc tataaaacca aaaactactg taataagtat
    12481 taatctttta tttaaagtaa aaaaaggtat attaaaaata cttttgtcgg atgtttctgg
    12541 agtataagaa tatataactc caaaaatcaa agtaagcata caaagtggaa gtagtgtata
    12601 acttgaaatt attcccaaaa atcctatact ttcaaaatat ttgctagcac tatttgtata
    12661 aacaaacatg tatccattta aatatgcgca tatacatgat ataatgccaa aagttccccc
    12721 taaggattga gtgtaccaat taaaaaattt agattccata tacctcacct cctaaaactg
    12781 tatatataat gatatgcatt taaatagtaa aatatatctt tatatgtttc tgaataaaat
    12841 tttgttaatt tcattaattt atgtttaaga aatttgaagt atagtgtagt atatattaat
    12901 gtagatgaag ttttatagta cttatttcat agtgcttaat caagttatct tattatggat
    12961 tcaaaagttg tttgtagggt tttatatttt gttgagctga aaaatttatt ataaatgaag
    13021 ggatgttttc gctttggtag atacattaga atttggtctt aaaattttat tttttatatt
    13081 atcaataatt tggatgggta aaataatgat tttaagaact gataagcaaa tagtaatcaa
    13141 tcctttgctt attggtatat ctgcaatcct tgtgatgttg catacctcac agagtaacat
    13201 tgaatttttt ggattagatg tccaatatat aagaatagtt ttgtatataa tatattcact
    13261 gataatttta ataggaatat gggctactaa cagaagaaat ggaatttttt agataatcta
    13321 tttctataaa atttaaaggt tgctagtaac aaatcactaa tattagcgtt ctgttactaa
    13381 caacctttat tttgattata cacttaaatt gaaaatagaa tgattgaata agttgacaga
    13441 ttattttaca ttgacagtaa ttagaacttc ttgagatgta tttaaatcat ttttgtcgct
    13501 tagtataact tttaccactg ctttacctgt tttattacct ttaattatca tatcttcaca
    13561 tgaaagtaca ctcttatcac ttacagatac aataaaattt aaatcttttg gaaggttatc
    13621 agtaacttcc cagtatttat aataactatc aattttcttc gtaattattt ttctcaaatc
    13681 aatactttca ccttttgcta tatcaacatt atgatattta gagtccagtt tatactgatt
    13741 atcttttgta tcaataaaat ttaaatcgca atttacatcc acaccattat gagaaagaga
    13801 atttactaca tcgtcatcaa tcaaataatt attggcaaga tttattgaat ccatatcttt
    13861 tagacttaaa gcttccttag gaacctcttt tatattatta ttccaaaaaa taaaattgtc
    13921 tacattttca agatatgata taccttttaa atttgaaata ctctcaccag ttgcataaaa
    13981 ttctccatct aaagccttta tattgcttat agtcatctca tctggattta tatatctttt
    14041 tacaactgct cttaaatttg catcaggaaa aatatcttta agttgatttt cgctcaaaac
    14101 cttatcattt gaatttattg gacttaaagc tgctgcagaa ataataaatg gtattaaatt
    14161 agctaaattc atagtaccct cccacataaa ttttttatta taataattta atttctatga
    14221 gagggttctg tttccttgtt atatatacct actttcgtac actttattaa tataaattaa
    14281 aataaatttc tactattaaa cttataaaca tattactagt tacgtttttt agcttcacat
    14341 ctaaatattc gcataccttg tgacataaat ttgtcttcat actctgttgt aacattattt
    14401 tcatactcac tatttcctaa atcaagagaa atattcttta atagccaatt atttgcagca
    14461 atttcattta aactaaactc gaataatttt tcattatctg ttttaaagtg aatttcgcca
    14521 tcatcttcca aaaccctatt gtacatttct aaaaatccag aatgagtgag tcttctttta
    14581 ctccatctat ttttaggcca tgggtcacag aaatttatat aaattctcga taattcttta
    14641 gcttcaaagt aatccaaaat attgcttaca tctccccata aaaatagtat attattcaaa
    14701 ttagaagcat ctgctttttc aacagctttt aaaagaactt cttccttaag ttccatggca
    14761 atataattta tgtcagggtt ctgcttagct aaagttgtta taaacttacc ttttccagtc
    14821 ccaaattcaa catgtattgg attatcattt ctaaacttta aattccactt gccttttaat
    14881 ttgtctatat cgtctcttaa aacatatttt gtataactta aaagcttttc atctgcacct
    14941 ttttttcgtc ttcttctcac aataattctc cttacatata aaaaattagt aaaatacatt
    15001 tttattatac attatctcac caaaaaataa aaagaactct cttaaaatag aaatttattt
    15061 ctataaagag agttctaaac ttactaaaaa cttaatctta gtggcaccct gaacaaccag
    15121 agcatcctcc tccaccttga tctttcatat tttcaggaat aactctaaat ccaaatcctg
    15181 tatcttctat tattatatct ccatactgac tatattcatc agagctcatt acaaattgta
    15241 attctccaac ttcataagtt aagtcatctt cttttttctc atctaaagct aatccaaaag
    15301 aaggacctcc acaaccaaca cctgcaaagt atactcttat attattaggt ttatcttgat
    15361 tgtcttttaa gatacttttt aaagtatcaa tagctgtttc tggtaaagta attttcatca
    15421 aatcattccc tttcaaatat tttattaaaa caatatctat tatataccca tacaaggtac
    15481 ataatcaatt agtttttgca tattttacta aaacttctac ctaatattac ctaaggcttt
    15541 aaaatatttt atataatatt ttattacctt
//
LOCUS       DAYATN010000091        15564 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.091, whole genome shotgun sequence.
ACCESSION   DAYATN010000091 DAYATN010000000
VERSION     DAYATN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15564
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.091"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            116..1192
                     /locus_tag="ACGI8A_002580"
     CDS             116..1192
                     /locus_tag="ACGI8A_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFH0180571.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1295..2137)
                     /locus_tag="ACGI8A_002581"
     CDS             complement(1295..2137)
                     /locus_tag="ACGI8A_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFH0180572.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2336..2893
                     /locus_tag="ACGI8A_002582"
     CDS             2336..2893
                     /locus_tag="ACGI8A_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0180573.1"
                     /translation="MKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYYHYASK
                     DDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVHMYLIC
                     EAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMSIVDGL
                     VVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3056..3328)
                     /locus_tag="ACGI8A_002583"
     CDS             complement(3056..3328)
                     /locus_tag="ACGI8A_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180574.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3455..4327)
                     /locus_tag="ACGI8A_002584"
     CDS             complement(3455..4327)
                     /locus_tag="ACGI8A_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0180575.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4623..7730)
                     /gene="ileS"
                     /locus_tag="ACGI8A_002585"
     CDS             complement(4623..7730)
                     /gene="ileS"
                     /locus_tag="ACGI8A_002585"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFH0180576.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7823..8055)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8230..8757)
                     /locus_tag="ACGI8A_002586"
     CDS             complement(8230..8757)
                     /locus_tag="ACGI8A_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFH0180577.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8792..9577)
                     /locus_tag="ACGI8A_002587"
     CDS             complement(8792..9577)
                     /locus_tag="ACGI8A_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HFH0180578.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9587..9844)
                     /locus_tag="ACGI8A_002588"
     CDS             complement(9587..9844)
                     /locus_tag="ACGI8A_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFH0180579.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9863..10321)
                     /locus_tag="ACGI8A_002589"
     CDS             complement(9863..10321)
                     /locus_tag="ACGI8A_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFH0180580.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10361..11065)
                     /locus_tag="ACGI8A_002590"
     CDS             complement(10361..11065)
                     /locus_tag="ACGI8A_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFH0180581.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11075..11710)
                     /locus_tag="ACGI8A_002591"
     CDS             complement(11075..11710)
                     /locus_tag="ACGI8A_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFH0180582.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12091..13131
                     /locus_tag="ACGI8A_002592"
     CDS             12091..13131
                     /locus_tag="ACGI8A_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFH0180583.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13594..14952)
                     /locus_tag="ACGI8A_002593"
     CDS             complement(13594..14952)
                     /locus_tag="ACGI8A_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFH0180584.1"
                     /translation="MVNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREK
                     IYNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDK
                     AAEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMD
                     AVKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGV
                     SGPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSL
                     APTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIP
                     VSEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGM
                     INNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSF
                     KN"
     gene            complement(14975..15244)
                     /locus_tag="ACGI8A_002594"
     CDS             complement(14975..15244)
                     /locus_tag="ACGI8A_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFH0180585.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
BASE COUNT         4948 a         2697 c         1798 g         6121 t
ORIGIN      
        1 aatataaaaa atatataaca aatttttttt atatttctag aagaaaagta tactcttttt
       61 tgtttaataa tgctaaaatt gatttgtatt gaattttgat taggaggaaa ttattatgaa
      121 agctcaaaga ttaaatgctg tacttgaaca aatgaaaaaa gatgatattt ctcaaatgtt
      181 agtaagtgac cctacttcta tattttatct tacaggagta ctaatccatc caggcgaaag
      241 actattagca ttatacttaa atttaaatgg caataataaa ttatttataa atgaattatt
      301 cccagtttca gaagatttag gtgttgaaat ggtttggttt aatgatactc aaaatccagt
      361 agaaataata actgaacata tagacaaaaa tgctactatg ggtgttgata aaaactggcc
      421 agctagattc ttattaaact taatagaatt aggtggagga agtaagtttg taaattcatc
      481 ttatataata gatactttaa gaatgtgtaa agatgaagaa gaaaaagagt taatgagaat
      541 agcttcaaaa ttaaatgaca aggctatgga acaacttaaa gctactgttt ctggtgaact
      601 tacagaaaaa caacttgttg gtaaattatc taaaatatat gaagacctag gtactgatgg
      661 attttcattt gaccctatta taggttttgg acctaatgga gctaatccac atggtgaacc
      721 tggaaatgca cttgtgaaac caggagatgc aataatctta gatataggtt gtataaaaga
      781 taactattgt gctgatatga caagaactgt attctataaa gaaatacctg aaaaaggtag
      841 agaaatattt gaaatagttc ttgaagctaa taagagagct gaagctattg taaaaccagg
      901 tgttagattc tgtgatatag atgctgctgc tcgtgactac ataactgaaa agggatatgg
      961 acagtacttc actcatagaa ctggtcactc tataggactt gaagttcatg ataaaggaga
     1021 tgtatcttct ataaatacag atactgtaca accaggaatg atattctcta tagagccagg
     1081 tatatattta ccaggtgagt tcggagttag aatagaagat ttagtacttg ttactgaaga
     1141 tggttgtgag atattaaaca aacatgacaa agaaatatgt gtagttggtt aattctaaaa
     1201 tcaatttgat aaaattaaaa agccatataa aaatgtttta aacattttta tatggcttta
     1261 atttctcaat ctatgattta tttcaatcca ttatttatat tgcagttatt aagattgcac
     1321 cctttgaagt atatgtcccc atcactgaac caatttcaca gattacaaca tctttgtatt
     1381 ttacatcctt ttccataact tcccttacct tttgagcctt ttctgggcaa ttagcatgtc
     1441 ctataaataa aaccttttct tctttatctt taatttttga ctctactaaa gtaatcagct
     1501 ttttcaaact gtttccttca cctcttgcct tatctatagg agttacaacg ccatctgcaa
     1561 cttgtattat cgccttaaaa tttaaggtat ttataatttt tcctgctatt ggatttattc
     1621 taccaccctt aatagcattt tctaaagttt caagtgttcc aaaaaatgga ggattatctt
     1681 tatatttatt tattattgaa acaatctctt caatttcttt tccttctttt attaatattg
     1741 ctgcttttaa cactaattgt cctattccaa ttgacccact aagtgaatct ataacctcaa
     1801 ctttcttgtt atttccttcc ttttcaaaca tatcttttgc taaaagtgca ctattataag
     1861 tcccagataa tcctgaactt atattaatca ctaatacact ttcttcctga caatggtatg
     1921 cttctaaaaa tttatcagga gaaggacatg atgtttttgg tagtacttta ctctcactca
     1981 tttttctata gaaagtttca ttatctattt cctttccaga tatataactt tcttctccaa
     2041 aagatacatt taaaccaaca acttctatgt tgtatttttc tattatatca tcaggtaagt
     2101 cacatgcact gtccactatc aattttattt tatccatatt aacctcacta taagatatta
     2161 ttttatttat tcagtcggtc gtacgaccga tttaattatt attttatata ttgaatgaaa
     2221 aaaagtcaat agatatagat tctcgcttta ttgacaataa atatccataa gcttataatg
     2281 ttattaattt gttataaata tacatggaaa ggtaggtttt atgaaaacaa ataatatgaa
     2341 acaggctatt ttatcatctg caaccaattt aatagccaaa aatggagttc aaaatactag
     2401 ccttgctgat attgcaaaag atgtaaatat aagtaaaggt actttgtatt atcactacgc
     2461 atctaaagat gatattatat atgatattgc ggatactcat ttagaagtta taactagtgc
     2521 tatactaaac tgtgtaaaaa atgtcaaatc aaaaaattct caaatagaaa tggttaactt
     2581 aatcttagag aaaatatcta ctatagaaag tcgtggtagg gtacatatgt atcttatttg
     2641 tgaggcaatt acttcaaata atgatttaaa ggaaagaata agattaaaat atattgaatg
     2701 gagaactact cttaaatctg aaattgttga atcactagaa aaatataata gtagcgaaaa
     2761 tgaacaggat gctgaatcat tttctttttt actcatgtca atagttgatg gtttagttgt
     2821 ccaaagttta ttaaaaacag aaaaaatacc ttatgaaaat atagcttctt ttttagttaa
     2881 tcattggatt taaacttaat acttagataa ttaataaaag tgaactattt aagttatcta
     2941 ctttaacagt tcactttctt atttattctc aataattttt acttaaaaca ttcaattttt
     3001 atttaaacct tcatggaatc acaaaataaa aatctcttat aaatttaaaa tgtttttatt
     3061 tatgttcatg tgtatgccca tctgaatgtt catgaccatg tgtatgtgta tgctcatggc
     3121 tatgctcatg agtacctgaa aaaccatcat catgattatg gtcgtgacct acaccattta
     3181 catgctcatg ttcatgccca tgactgtgag aatgttgatg tgaatgttca tggttatctc
     3241 ctccacagca tccttctcct ccagtcccac aacacccact tgtatgtgga ttatgatgat
     3301 ttcttactac aaatctctct cttaacataa ttcttatacc tcctagtata ttattgagta
     3361 cttcatgtac cccattaact acagtataca tctaactagg tataatctca atacatatgg
     3421 cattttttaa catatataat taacatatat aatcttaaag aaatttatcc ttaaaacctc
     3481 caccttttac ttcttcaacg ttattgacag aaataaaagc ttcactatcc atatcataaa
     3541 ttaaatcttt aaatttacct atttcatttg cagatattat gcaataaatc atctttttct
     3601 tattttgtgt atatgcacct tctgcctcca aaaaagtgac tcctctaccc attttattca
     3661 ttatcacttt ggatatttca tcatatttat ttgatattaa aagtatagat ttttttctat
     3721 caaagcaatc ttttcctata tccatagtaa aagaaattat atacattgta attaaagtgt
     3781 acatcaccaa tttagctcca aataaaaaag ctcctgcaca tactacaaaa aaattaataa
     3841 ataaaaatgt atttttcata ggtatatcat actttctttt cattatggct gctattatat
     3901 caagtcctcc agcagtagct cttgctttaa aagttaaccc catacctata ccagttaatg
     3961 caccaccata aatacactgt agcatagtat catctattgg gaaatattta gcaatatctt
     4021 gtgttagccc cagtgctaga gaaaatagta acatattgac aaaacttatt agcaaaaatt
     4081 tattatcaaa atactttcta gctataataa atattggtat gttcatcaag aaacttgata
     4141 ttccctgatt aataccaaat aatttgtata atataacaca aattccagct actcctccac
     4201 ttaataactt ggctggtgct agatataaat ttattcctaa ggttgataga atcataccca
     4261 atataataag acaatattgt aaaaatatat tattatcaat acttagcttt ttactttcac
     4321 catacatcaa aacgcacccc tttgcataat aaataaacca ccctgcataa cacacgataa
     4381 atttacaaat aaatttatca gaagtagttt cctaaaaata ttattcatcc tcacttaaac
     4441 gtttattcat taatattcta tcacatgaca taaataattt atatgattta tatagattta
     4501 tttattgtaa attttggaat attaaaacat tttattgaat agataattat attacataaa
     4561 aaatccctat agaaatctat aaactaattt ctatagggat tttggttaac taactattat
     4621 ttttattttt ttataacttt tatacctgtc atatggtcat ttaagttttg ttcctctaaa
     4681 gatttatcat ctactctgtt tatttctaaa gctaaagttt cagacttgat atattcatta
     4741 aaatggtcaa cagccttagc aatttcatca tcaccacagt attctattac tatattatct
     4801 aatacttcaa agttattaga ttttcttaat tgctgtactt tagatataaa ttctctagca
     4861 taaccttcat taattaactc ttcagttagc ttagtatcta gtataacaaa aagattgttt
     4921 tcaacagata cattaaatcc ttccttagca gttataccta ttaatactaa atccttatta
     4981 aattcaaaag cttcaccatc taaatcaact gttaaagtct cacctttttc aagtttatta
     5041 gcagtttcat gagcatttaa tttacttaat gctccaacga ataaattcat cttagaacct
     5101 aataatggcc ctgcttcctt aaagtttggt tttaaactaa agttcatata ctcatctaaa
     5161 tccttagcaa atattacctc tttaacatta agctcttctt ttattaaatc aactacatca
     5221 cttattgtat cttcaaactt accatctact aacacttttt gtaatggttg acgaactttt
     5281 attctttcta cttctcttga agctctacct aaagtaacta agtttttagc taaatccatt
     5341 ttttcttcta atttagtatc tactaactct tcattacatt taggatattc agctaaatga
     5401 acagatactt ctcctgttaa gtttctatac atttcttctg acatatatgg tgcaaatgga
     5461 gctatagctc tacaaagacc atgtaacaat tcataagttg tattgtacac tgctttttta
     5521 tcttctgtta attcagttgc ccagaatctt cttcttgaac gtcttatata ccagttagat
     5581 aaatcatcat ttataaaatc ttgtatcatt cttaaagttt tatttaactc aaatatagct
     5641 aaattttctt ctacatcttt cattaaatta ttgaacttag ataatatcca tctatcaagt
     5701 tctggtctat ctttatattc tacaaaaaat tcagttggat ttatatcatc tgtgtttgca
     5761 tataaagtaa agaagttata tacattcttc atagtaccta taaatttact ttggatttct
     5821 tttaatccat ccatatcaaa tttagttgga gtccatggtg gagaaacata tagtaaatac
     5881 catcttaatg catctgctcc atattggtca aataattcca ttggatttac tgtatttcct
     5941 cttgatttac tcattttctt accatcttta tcaagtacaa gatctggaac taaaacactc
     6001 ttatatggtg cttttcctaa tacaaatgtt gatactgcaa gtaatgagta gaaccaacct
     6061 ctagtctggt ctataccttc tgatatatag tcagctggga acaattggtc aaagttttcc
     6121 ttattttcaa atggatagtg gtgttgagca aatggcatag ccccactatc aaaccaacag
     6181 tctataacct ctgttactct tgtcattggt tttccacatt tttcacattt taagtgtata
     6241 tcatctacat atggtctatg aagttcaact gtttctggat ttacatcttc tatagctttt
     6301 tcagctaatt cagctcttga accaactgaa tctgtatgac cacattcaca tctccatata
     6361 ggaagtggag ttccccaata tctagttctt gatattgccc agtcatttag attttctaac
     6421 cagtttccaa atctcttctc tcctacaaaa cttgggaacc attttacacc attattattt
     6481 tctattaatt tatctttaag tttagtcatc tctatatacc agcttggttt tgcatagtat
     6541 actaatggag tttgacatct ccagcagtgt ggataattat gaactacttt ttctttgcta
     6601 tataatttat tttcagaagc tagccatttt attatttcaa catctagccc atcttccatt
     6661 acaaatctac cttcccaagg agtagatgta aatttaccag tttcatctac tggttgaata
     6721 actggtaaat catatctttt acctgtgttg tagtcatctt caccaaatgc tggtgctgta
     6781 tgaactatac cagttccatc ttcagtagtt acataatctc ctactgtaat aaaaaatgct
     6841 tttttgtcaa cttctacaaa aggcattaat tgttcatatt ctaaaccttc taggtcttta
     6901 cctttcattt cttctaaaac ttcataatct tcacctagta ctttatcagc taaaggttta
     6961 gatacataat atacctcatc acctttttta acttttaaat aatctacatc agcatttact
     7021 gttaatgaaa cgtttgatgg taaagtccat ggagttgttg tccatgctaa gaagtattca
     7081 tcagcatctt ttttcttaaa tttagctata actgtattag tttttacttc tttatatcct
     7141 tgtgcaacct catgactagc aagaccagtt ccacatcttg ggcaataagg aagtatttta
     7201 tgtccttcat atatatatcc ttctttgttg aatttgttta gtatccacca tacagactct
     7261 atatagttat tatctaaagt tatatatgga ttatctaaat caacttcata agccattctc
     7321 tcagtcatta ttctccattg tttttcaaat gagaaaactg actctctaca tttttggtta
     7381 aattcagcta ttccatattc ttctatttgt tgcttgctag taagacctaa ctctttctct
     7441 acttgtattt caactggtaa tccatgtgta tcccaaccag cttttctctt tacttgataa
     7501 ccagacattg ttttatatct acaaactgaa tctttaagcg ttctagataa aacatggtgt
     7561 actccaggat ttccattagc tgttggaggt ccttcataga atacgaaact tgggtcatct
     7621 ttcccttttt ctaaagtttt ttcaagtata tttatgtctt tccaataatc aaaaacttgt
     7681 gcttctgctt gttttacaga actgtctacc aatggcttaa atttcgccat gtaaatcacc
     7741 ctttcctact aaatattata attccaaaat tttttattct ttcacataaa tttaaatact
     7801 aaaaaactcg ccccctttaa accaaaggga cgagttataa atcgcgttac caccctaatt
     7861 ctatatgtaa actaaatact agctacatat aacactctaa tagtataacg gtactaaccg
     7921 gcacagctta gtagaattta atcgttcagc ctgcagctca agagtgattt ttatctttat
     7981 aaattcattg gttctcagca tacccaattc tctgtaaaat tatctaaaga taaatgtctc
     8041 tatcaacgct tttaaaaatt tatctgtgtt tatatgaaat tctataacaa ataatttgaa
     8101 tagtcaatat atttttgaaa aatataataa tttctatttt tcttacattt ttatcctatt
     8161 tttaatcttt gttatagatt tctcaaactc acacaattat tctacataaa aaataaacaa
     8221 attacaagct tattctaaag ttgtagcagc ttcatcatta ctataattat acactgttgt
     8281 tccttcaaat gcatttacac atttttcatc tacatcataa aaaatctcat caacacttct
     8341 tatttcatct tctaacagtg atttaaattt atttctaaat atcttaaatt ctttgactaa
     8401 agcatcatac tcttttctta tctctataac acggttatta gcttgttcta taatttgtct
     8461 agcttttaaa tcagcttctt caacaattat cttagccttt ttattagccg cgctacaagt
     8521 gtcttctgct gcactttgag cagttattaa agtagctttt agtgtttctt caatattttc
     8581 atacttgttt acttggtctt gatataaccc taatttttct ttaagagctg tattttccct
     8641 acatagagat tcataatctt ccttgatgat atctaaaaat tcatcaacct cttcatctct
     8701 ataccctctt aaaccttttt taaactcttt attttctatc tcaattggag ttagcattac
     8761 atttcctccc agctataatt taaattagtc cttatacaat taatcttgct tgaactttta
     8821 tttttccttt tttagtaata tctcctattt gtgttacctt agctcttcct ttgcctctaa
     8881 tagaaatcaa atcatcgttt tttactattt tagaagttga agttatcttt tcataattga
     8941 catggacttt ttctccattt atgtattttg cactatcttg tctagatata ttgtaaatac
     9001 cacttatcac acaatctaac ctatctgaag atactgtaaa tgatacttct ttatattttg
     9061 aagaactatt aacaatattt tctctggaaa tttcctttac aattacatta tttcttgaaa
     9121 ccttttctaa gtttacaatt ataaaatcgc aaatatcact acttacaact acttgacaaa
     9181 agctatcatg tataattata tctccaattt tttctctttt tatacctaga cccagtattg
     9241 accctaagta atctttatga gatatatttt taaatttgaa attcccttct atttgtaaaa
     9301 atctaagagt atcttctata tcttcatatt ccatgtaaaa tggataaata aatacagtgc
     9361 ttctttcagc ttgttcataa cctccatcca cactgtattt tatttcattt gtagaattta
     9421 atattgctac agcatttttc acttcatatg gattaagaaa ctccgtagac ttaacatcat
     9481 agttttttat acaactatta gccttgtcta tcactttaaa cattttattt ttcaagtcta
     9541 tatcctttat gtgatttgtt agttttaatt tatccatcct atcaccttag aatataacca
     9601 atactaattg tcttaggaac atcaataata ctattgcaat taaaggagaa aaatctatag
     9661 gtccatttgt atatttatac attatagacc ttattggccc ttctattggc tctgtaagat
     9721 tttccaaaat atcaaaaatt ttactttgtc ttccatttgg aaaccaagtc atcaagcttt
     9781 ttataactat catccatgct attatatcta aaagataata aagtgcaatt cttattgttc
     9841 ccatatatta accgcctttt tactatttat tttgccaagg gaagaatgct ttactttcta
     9901 attctttttt catatttgca tctatatcca cattattagg agctagtata aatattgatt
     9961 tagaaacctt ttgtatactt ccatctaaga cataaactgc cccattcata aagttaaata
    10021 tagatttacg aacttcagaa ttagtcaatc cttccaaatt cactataact gcccttcttt
    10081 gttttagatg gtctgctata gttgcagctt catcataaac cttaggttca actattacaa
    10141 ctttcatttg gctagttgta tgtaaattta caattttatt tgcttttgca gtactaaagc
    10201 cactgttcat ttcttcttct tgaactatat cgtccatgcc actttcatat tcatcttcaa
    10261 tgtaatcttc ttcttcatca actatccaat tcttaaattt agatattatt ccatttgaca
    10321 ttattaactt cctccttaaa atttattcaa atttgagtaa ttatttttta ttgtagtttc
    10381 tttctccaaa gattgatgtt ccaactctaa caatggtagc cccttcctct atagcaattt
    10441 cataatcgtg actcattccc attgacaaaa catccatccc tacattaggg atgtttagtt
    10501 cttttatttg ttttgataaa tctttaagac ctttaaatac acctcttatc tcatcttcat
    10561 cttctataaa aggtgccatt gtcattagac cctttatttt tatattttta tatttttctg
    10621 acacttcttt gacaaagtct acaacttctt ctctatcaag tccatgctta gactcttctt
    10681 tagatatatt cacttgtact aagcaatcta tttctctgtt tatttgttct gcacgttttt
    10741 gaatttcttc gcataatgca attctatcta aagagtgtat catacaaact ttatcgatta
    10801 tgtacttaac tttattggtt tgcaaagttc ctatcaggtg ccagtttaat ttatcaccta
    10861 taatatcata ctttctagct aattcttggg gtttattttc accaacagaa gtgattcctt
    10921 ccctcattgc ctcattaact aagtctacat caacagtctt tgtgactgcc aaaagggtaa
    10981 tctctttgct gtctctatct actttttttg cagctttatc aattttgttt cttatctcag
    11041 acaaattatc tttaatagaa cccattatta tcacctactt tatccttata tttgtgttta
    11101 tactgttagg tcttaaaatt attttgtcat atagattaac acttgtaact ccatttatgt
    11161 catttttata ataatccaca aatacagaag attcattctc ataagttatt ccttctaata
    11221 tgacaaattt tacactttgt gattgttcat caattacata tacaccacgt ttattgtctt
    11281 ttgtttttat agaagtttta ggtattttta aacattccat ctgattatat attatatcaa
    11341 attcttcgac acttgtatcc tgatttgcaa caatttggtt acttattttt aattttacca
    11401 ttattttatt attattttca tatattttgt aaacttttgc atctagtttt tgctcatgat
    11461 attcaattat tacattttga tttttttcaa agttattaga atcttcttta ctgatgtatg
    11521 tagatagata attactttgg tcattggcat ctttttctat agttcctttt acatgaatct
    11581 ttgcatcaac tttttcattt tttaacacca acgtatctac atttttgcta attatcatta
    11641 ctaatatatt aaatactaaa tatataagaa taccaagtat cactaatctg gtatatttta
    11701 cctttttcaa agactctccc cctataaaat tactttcatt ataagtactt cttctaaaaa
    11761 atgtttattc cttttttata agataatatt ttacgttcta ataattattt tttccatatt
    11821 aaatttattt tttatcaatc ttcctataca agactgcatt atttatatat aattgtatta
    11881 taaagtataa gtttaattta cattttatac gttaagctta ctttatatat ttttatagtt
    11941 atttttattt ttagatgcaa tataaaacca attaagattt aaaataactt ttgttatgtt
    12001 gacatttatt taggtaatca tttatgaatt aggataacgt tatcacaaca aaagtttatg
    12061 tcctaaaaaa aatacaagga ggattttctt atgtcagaaa ttttattagg aacaggttta
    12121 cttttattgg ttctatgttg tttcacaatt tttagttaca aggccccata tggtatgaag
    12181 gctatgggag ctctagcaaa cgctgcatgt gctactttct tagtagaggc attcaacctt
    12241 tctttacttg gtgatgtttt aggtatggaa ttttttagaa gtgttggagc tgctaatggt
    12301 agtcttggtg gagttgctgc tgctatactt gtgcctttag ccttaggtgt atctccagtt
    12361 tatgcagttt taataggatt atcttgttta gatttttcta tattatctgg attcgttgct
    12421 ggatactttg tttctttctt aattaagttt atagagaaaa aagtgccacc tggagtggat
    12481 ttagtggttg taattcttct atgtgcacca ctatctcgtc ttatagcatc aaatgtagac
    12541 ccattagtta atagtacact actacaaatt gggcaagttt tgatagaagc taccaacatt
    12601 agccctataa taatgggaat tatactagga ggtctaataa cagtagttgg aacgtctcca
    12661 cttagttcaa tggctcttac atctataatt ggtcttactg gtcttcctat ggcaatagga
    12721 gcattaggtg cttttggttc agcttttatg aactcaacac tatttgcaaa attgaaatta
    12781 ggttctaaaa aagatattat atctgttgct attgaaccat taacccaatc agacataatt
    12841 tcagctaatc caatacctat atattctact aatttctttg gaggagcctt ttctggtatc
    12901 attgtagcct tgatgggtct tgtaaacatg acaccaggta cagctacacc tgtggctggt
    12961 tttgctataa tgtttgctta taatccagtt caacaagttc ttataacagc tggtttatgt
    13021 gctttattca atgtaattgc aggattttta ggttcaaaaa tattcagtac ctttaaagtt
    13081 cttactgctg gtgaaatacg tggtactgat gtagaagaag aaattgctta aaaaacaata
    13141 agatttataa atttatatct caagtagtag agttttatat tgtaggcaat tttattaatt
    13201 gcaaacgttt tattttttat ttatgtttat gattattatg tgtgttgaat atttagataa
    13261 atttttattt attattttta tttagatttt tgtttagttt tttatttggt tgatatatta
    13321 ggaataaaaa ttagtatagt ttattactag ttagatggca taacttttta gatacataaa
    13381 aaattttata ttaattagat attattaaaa taaaagcgat acatgaattt ttaaatgtcc
    13441 atgtatcgct ttatttttgc cataatctta ataactgatt tttaaagttt attatcttaa
    13501 caaagttcat tatcttatat aatatttaaa tgcataggct tctttaattt aaccatctaa
    13561 atatttaatt aaatattatt atatgtaata taactaattt ttaaatgaat gtataggagc
    13621 tggaatcctt cctcctcttt gagtaaataa ttcagatgaa tttgtattta taggcattac
    13681 tggtgctcgt cctaaaagtc ctccaaattc taccatatca cctacatcac atcctggggc
    13741 tggtataatt ctaacggctg tagtcttatt attaatcata cctattgctg cctcatcagc
    13801 tatcatggct ccaagcgtac ctgctgtagt atttcctggc acagctatca tatcaagacc
    13861 tacagaacaa atagcagtca ttgcttcaag cttatctata cttaaagcac ctttaataac
    13921 agcatcaatc atacctgcat cttcagatac tggtataaac gctccactaa gtcctccaac
    13981 atgtgaacat gccattactc cacctttttt aacagcatca tttaaaagtg ctaatgctgc
    14041 tgtagtccca tgagtaccaa caacttcaag acccatttcc tctaatatat ttgctactga
    14101 atcaccaata gcaggtgtag gagctagtga taagtctatt atcccaaatg gaacatccaa
    14161 tctactagaa gcttcctttg ccacaagttg tccaactctt gtaattttaa atgcagtctt
    14221 tttaactgtt tctgaaacaa catcaaaagg ctctcccttt actttctcta aagctctctt
    14281 aacaacacca gggccactta caccaacatt tataattcta tcagcctcac caacaccatg
    14341 gaaagctcct gccataaatg gattatcttc tactgcatta caaaatacta ctaattttgc
    14401 acaaccaaaa ccttttgcat cttttgttaa ttctgcagtt tcttttatga tttctcccat
    14461 ttctttcact gcatccatat ttataccaca tcttgtagaa cctacattta ctgaagcaca
    14521 tactttatta gtacttgcta gagcttttgg aattgactta attagtatct tatcaccttt
    14581 tgtatatccc ttttgaacta atgctgagaa tccaccaata aaatctatac ctaaatcctc
    14641 tgcagcctta tccaatgtct gtgcaaactt tacatagtca ttttcatctg ttgcacctgc
    14701 aataatggaa attggagtta ctgataccct tttatttaca attgggatac caaattcacg
    14761 ctctatatct tttccaacct tcaccaaatc tttagcagaa ctcataatct tattgtatat
    14821 tttttctcta gctttatctc catcagggtc tatacaatct aataaagaaa tccccatagt
    14881 tatagttctt atatccaact gttcttcatc tatcatcttt attgtttcaa gtatatttcc
    14941 tacattcact attatcacct ccccttactg tctactacag attatgcatt gaattaaata
    15001 tttcttctcg ctgaattttt acatccactg ataatttctt acctctttca accaagtcct
    15061 caaccgtttt ctcaaaagaa tttaatgatt tctctaaatc aactatcata atcatagtaa
    15121 aataatcctg taatattttt tgtgatatat ctactatgtt gatattttgc ttgtataatt
    15181 catcactaat tcctgccact atacctggtt tatcctttcc aattactgtt aaaattgctt
    15241 tcataataat atcctccctt atttttttat aaatcttata tttttacaaa ttgaactatg
    15301 ttttaacata tatttcattt atttgttagt aaaccacttt ttatgtatat atttaatata
    15361 ttttaatacc tatacattca tttgtcaatt ttttttcaat attctattat ttttatacta
    15421 tttagaaaat tcatatatta tatatgtttt ctcaacaata aatacagttt tcataataat
    15481 gaaaggtatt acttagcaat cgcatgattt tctaaataat acctttctaa aaattatttt
    15541 tttaatttta tgaatttaat tata
//
LOCUS       DAYATN010000092        15508 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.092, whole genome shotgun sequence.
ACCESSION   DAYATN010000092 DAYATN010000000
VERSION     DAYATN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.092"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            136..843
                     /locus_tag="ACGI8A_002595"
     CDS             136..843
                     /locus_tag="ACGI8A_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180586.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            890..2485
                     /locus_tag="ACGI8A_002596"
     CDS             890..2485
                     /locus_tag="ACGI8A_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180587.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLMVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            2588..3670
                     /locus_tag="ACGI8A_002597"
     CDS             2588..3670
                     /locus_tag="ACGI8A_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HFH0180588.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            3819..3980
                     /locus_tag="ACGI8A_002598"
     CDS             3819..3980
                     /locus_tag="ACGI8A_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HFH0180589.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            4321..5589
                     /locus_tag="ACGI8A_002599"
     CDS             4321..5589
                     /locus_tag="ACGI8A_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFH0180590.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            5784..6572
                     /locus_tag="ACGI8A_002600"
     CDS             5784..6572
                     /locus_tag="ACGI8A_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFH0180591.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSIRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            6572..7021
                     /locus_tag="ACGI8A_002601"
     CDS             6572..7021
                     /locus_tag="ACGI8A_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFH0180592.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            7025..9289
                     /locus_tag="ACGI8A_002602"
     CDS             7025..9289
                     /locus_tag="ACGI8A_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFH0180593.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            9429..9740
                     /locus_tag="ACGI8A_002603"
     CDS             9429..9740
                     /locus_tag="ACGI8A_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180594.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            10026..10526
                     /locus_tag="ACGI8A_002604"
     CDS             10026..10526
                     /locus_tag="ACGI8A_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFH0180595.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            10583..11569
                     /locus_tag="ACGI8A_002605"
     CDS             10583..11569
                     /locus_tag="ACGI8A_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0180596.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            11619..12341
                     /locus_tag="ACGI8A_002606"
     CDS             11619..12341
                     /locus_tag="ACGI8A_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HFH0180597.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRNLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            12370..14304
                     /locus_tag="ACGI8A_002607"
     CDS             12370..14304
                     /locus_tag="ACGI8A_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0180598.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            14297..>15508
                     /locus_tag="ACGI8A_002608"
     CDS             14297..>15508
                     /locus_tag="ACGI8A_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HFH0180599.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLD"
BASE COUNT         6148 a         1444 c         2679 g         5237 t
ORIGIN      
        1 atatttatac ctttatattt ctgatacctt tgcattttat agatttctaa aataaacaaa
       61 taaaagttgt aattgtttga tatttgttat atattatata taacaaatat taaaactata
      121 atatggaggg aaattatgtt aaaagtaatc aatgtatcta aatcatataa aaataaaaaa
      181 gtagtaagta gtgtctcatt tgatgtaaat gatggagagc tttttgggtt tataggacat
      241 aatggagcag gtaaaactac tactattaaa gcaattgttg gaatacacga ttttgaagag
      301 ggagaggtac taataaattc taaatctata aaaaaacagc ctgttgaatg taaaaaggaa
      361 atggcatata tacctgataa tccagattta tacgagtcat taactggtat acaatacctt
      421 aattttatag ctgacatatt tgaagttgcc aaagataaaa gagaagagtt aataaaatat
      481 tattcaaata agtttgaact caataaagca ttaggagatt taatatcttc atattcacat
      541 ggtatgaaac aaaaattagc tataatttcg gctttgatac acagcccaaa aatcttaatt
      601 cttgatgaac cttttgtagg tcttgaccca aaagcttcct ttatattgaa ggaaattatg
      661 agggagtttt gtgacaaagg tggttgtata tttttctcaa cacacgtttt agaagttgct
      721 gaaaaaattt gtgataagat tgccataata aaagatggaa atataattgc ttatggaact
      781 acagaagaag ttaagggaag taattcactt gaaagtatct ttatggaatt aatagagaaa
      841 taaaatgttt tttatacaat atagtgtcta aagtgaggaa tgataaaata tgagcaatct
      901 aagtatatta ataaaaacta atataataaa tgaatttaaa ttaaatgcgt taaaaaaagc
      961 tgatggtaga gaaaaacaaa aaataatggg aatgatgctt tcagttgtta taatagcggg
     1021 tctattaatt ttttatgtaa caagcttaag tctctcttta gttgatatct taaagcaaat
     1081 aaatcaactg gaaatgctac tgatatttgg ttttggatta tcaacattaa caactattgt
     1141 tacatcatta tataaaacgt catcatatct atttcaagca aaagatttag accttttgac
     1201 tagtttacct ataaaagaat ctactgtact agcaagtaag atattaatgt tggttggagc
     1261 aaactattta ttttcttcaa tatgtatgat tatacctgca gtggtttact ttttaaattc
     1321 tgatgtaagt ataacttatt tcccttattt gatattgcta tttttagctt taccattatt
     1381 accgatagtt gcttcatcaa taatttttct atttataggg cagatatcat caagaataaa
     1441 atacaaaaat ttagtattaa tcataggtag cattatattg atgttgcttt ataccctact
     1501 tatgtcaaga cttaattcta tgtcagttga aatgctaaag aatagtgtgt ctatttcaga
     1561 gacaattaga aaagtatatc ctcctatata ttattttgtt gatgctctta aaacaggaag
     1621 tatagtttca tttttagcat ttatagccat ctcaataata ccatttttta tgtttgtaac
     1681 tctatttgca aaaaaattta aagatataaa tgctaagatg agtgaaggat ataagtcaaa
     1741 aaattatgaa tttaagggtc aaaaaacttc aagaccttca aaagctttat taaaaaaaga
     1801 atttaagaga tatacttctt cgtttatata ctttttaaat tcatctgcag gtttgttatt
     1861 tctaatggta ggtacaattt taatattatt atttggagca gataaagtgg gtgagttact
     1921 gaaattgaat atagatttta atcttataaa aatacaatta ttgggagtat tcttctttat
     1981 aatagtaatg aattgtacaa catactgttc aatttctctt gaaggaaaaa acttatggat
     2041 actgaaatct tctccaatag atgaaagaga aatattctgg tcaaaaatat tgatgaattt
     2101 tttattgaat gcaccactat ctattttatg tctaatatta gtatctataa gattagactt
     2161 tgaaattcag tttattattg tatcaatctg tttgattgta agtttttcaa tgtttgtagc
     2221 attgatggga ttattatcaa atttattata cccaaacctt gattggaaaa atgaggtggc
     2281 tgtagtaaaa agaagtgcaa gtatgatagt tacaatgtta gtatcattag cttatgtaat
     2341 tatacttgga ggtatatatg tattacttgg aatttcgttt ttaaatatat atattatttt
     2401 ggcaagtata cttactttga tattagattt tatgatatgg agaattattt ctactaaagg
     2461 agtaagaata tttaaaaatt tataggtgta tttatgtcaa ataaagactt atctttttta
     2521 gtcttgcaaa taataggtta agtagtatac tatgtatagt gatatgaaaa aatttaggag
     2581 aaaataaatg agaaaattat ccaaaagaga aaaagatttt attcaatatg gatttttatt
     2641 atttttgata tgtattacta cttatttagt attaactact ttagatataa aactaatacc
     2701 taaaattata aggctagtaa ataagactta tatatttctt ggcatattga caattatagt
     2761 atatatgata attgaatcta ttgtaactca tttgataata aatgcagttc aaaaaacaaa
     2821 agtaaaattt attggtatta aaatggctac aatgggattt tactataact tagttactcc
     2881 atttgcatct gggagtcaac caatgcaaat atatgcactt acaaaatatg atgtaagcct
     2941 tagtaaatca gtggctatag ttactaataa aactgtagta tttcaaagta cagttactgt
     3001 attttgtgga attttaattt tgctaaataa gtcattactg atggagcaag tacattctgt
     3061 gaaagtcttg ataacttttg gaatgattat gaatgtaagt atgttgttag gtggactact
     3121 aatagtattt agtccaaaac aagtaaaaat gatagcagaa gttttgatta attttttatc
     3181 taaatttaaa acatttaaat ttttagattc aaaacgtgat agaataaatc attatattga
     3241 tgattataat tattctataa aaatatttat taaaaatgta agaatcttag ttttaagtat
     3301 attacttact gtgttacaat taatcgcata tttcagtata gtttattgtg tgtataaagc
     3361 ctcaaattta agaggagttt catatgtaca tataatgaca ttacaggtct ttttatatat
     3421 ggctatatca ccaattccaa caccaggaaa tgttggagct aatgaggtta cattttttac
     3481 tatgttctcc aatataattc caaaagaatt attggggtac tctgtactct tatatagtgg
     3541 gtttgtatac tattttatat taatagtttc aggtttattt acatttggaa cgcattatac
     3601 attaaattct tggaaaaagg aaaataaagt aaattctaat ttagataagg ttattgaaaa
     3661 attaaattaa taaaataatt agggaaattc tatatataaa tatggtgttg aataagctat
     3721 attatattga gatatttccc ttttttgatt aaaattatag aaatttgaaa taataagtaa
     3781 taaacagaaa aagtgatatt gttttgaaag gagaaactat gaaacaagat aaacaaaatg
     3841 aaaaacaaag tgagatttct aagataaaac aaagcaatga taataaactt gaaatagctc
     3901 aagaattaca tgtaagtaaa aatataggga atatgcttgg atatagaata gataagaaaa
     3961 aaatgaatag accaagttaa tgaaaatgtt gaaaatggta cattttaaaa aatatatata
     4021 atgaaatgtt ttattgttaa atttaaactt attttaatgg gggaaaaagt cgaaaaatat
     4081 ctaaaaagat atggatataa aaaatattaa tatatatata ctataaactt gacaaaatca
     4141 accttttaat aactagcaaa aaatagcaaa attatataag cgttatttaa ttgtcaaaaa
     4201 tgaaagctaa attgcatgtc ctaaaaaaaa tgattgataa catttatttg tgtgctataa
     4261 ttatatagta aaacaataag tatattgcat aatgtatgca aatcgggagg agagacggac
     4321 atggaagcat ctatacacac aattgccagc aataatttat tgataatttt tgctattgta
     4381 agtataactg gaataatttg tagtaagctt agtgaaataa taaaggttcc agatgtagtg
     4441 ttatatttac ttgtaggtat tttaatagga ccatcatttt taaaatttat agatattcgt
     4501 ggatttcaaa tagaaaatca actaatactt acatttggtt ctgcatttat tttatattta
     4561 ggtggaaaag aaataagttt aaaagtattg agaaatgtga agatatctgt attcttatta
     4621 gctactttag gagttgtgat atctgctttt ataatgcaac aagtaatagg atttactttt
     4681 gggataagtg caatgactgc tttactagca ggaactataa tagcatcaac agacccagcc
     4741 actcttgtac ctatatttaa tcaggtaaag ataaaggata gggtaaaaca aactgtaata
     4801 agtgaatcag cttttaatga tgcaacagga gctatactaa catcagcagt gttggctgta
     4861 attttgtcaa ataaattttc attaggtgaa aatatttatg aactggggac aatggttatt
     4921 gtcggagttt tagttggttt aattacagga atactgcttt tgaaattagt taatgataaa
     4981 ccttatggaa tatttaaaga ttttgcacca ataatatctg ttatttcagt tgtagttgca
     5041 tatgaattat ctacaaaatt aggtggaagt ggatatatgt cttgctttat tgtaggtata
     5101 ataactggta acaagaaaaa ttttaaaatt tggttaagtc aaaaatctta tgatgcagat
     5161 ttttatgttg ctgaaacttt aggtactata tgtcgtatgg caatatttat aatattgggt
     5221 agccaagtta atttggctca gttaagtaaa tattttatgc catctgtaat tacagtatta
     5281 gtattaatgt ttatagcaag accactttgc gttttagttt gtacacttgt agacagggaa
     5341 gcaaaatgga gtaaaagtga aatattattt atgatgtggg taagggaaac aggcgtaata
     5401 ccagcagcac tttgtggtat tatttcagca atgaaagttc ctggatacga agtgatttca
     5461 tctgtagtat tcatgactat actaattaca ttagtaatac aaggaagtac tacaaaatta
     5521 gtagctaaaa aacttggatt attagaagaa gaagtgataa atataagtga aaaagtatca
     5581 actttttaaa gttttgcttt aaaatagtac ctgttaatgg ctattttaat attgtctaat
     5641 gatgattatt gaaatagtca tttttttatg tataagtaat ttacagaatt tacatattag
     5701 acatatttat tatcaaaatt tgttgaagct atgatataat tagttgtatt taaaataaga
     5761 atagcaaata agggaggaaa tttatgttta cggttatgga tattgtacag ccagatacag
     5821 tagaagaagc gtactcaatt ttaaataaaa gaaaaactaa ccaagtaatt ggtggaagtg
     5881 cttttttaag aatggggaaa aaaagaatag gaacaggaat agaattatct aaattaaatt
     5941 tagattatat aaaagagtat gaagattacg ttgagattgg ttctatgact acttttagaa
     6001 cattagaaac aagttccata ataaaaaata attttggaag aataatagaa gattcagtta
     6061 aaaatataat aggagttcag tttagaaatg tagtaacagt tggtgcaaca gtattttcta
     6121 aatatggttt ttctgacttg atagtagcat tactttcatt agatacggaa gtagaattat
     6181 ataatattgg gagaattagt cttgaagagt ttctaaatag agattatgag aaagatttac
     6241 ttattaagat atacataaag aagacaaata agaatgcttc ttataaatca attagaaatg
     6301 caaagagtga ttatccaatt ctaaatgtat ctgtatcaaa acatatggaa caatttaagt
     6361 tatgtgtagg agcaagaccg cagaaagcta cgattgcaaa acaagctagt gaatttctat
     6421 ctaacaatga aatcaatgaa attaatatag ataaagcagt agaaatagca tcagaagaac
     6481 taacttttgg ttctaacatg agagcatcta gggagtatag aaaagctatg agtaaagtac
     6541 ttttaaaaag agctataatg gaggttattt aatgttagta aagattaata taaatgggaa
     6601 gaaaaaaagt gttgatatag aaccagaaga atatctggtt gatactttaa gaaaagttgg
     6661 taatttaagt gtgaaaagag gttgtgacac aggttgttgt ggtttatgta cagttttaat
     6721 tgacaagaaa cctacattat cttgtgcaac actgacattg agagctttag ataaagaaat
     6781 aactacaatt gaaggtttag aaaatgaagt taaagaattt agtgaagttt tggtaaaaga
     6841 aggagctgag caatgtggtt tctgttctcc tggctttata cttactgtac ttgcaatgaa
     6901 ggatgagctt gaaaatccta cagatgatga aataaagcat tatcttacag gaaatctatg
     6961 tagatgtaca gggtatatgg gacagttaag agctatcaaa acttatctgg aggtaaaata
     7021 gaacatgaat atagttggaa aaggaatcaa gaaaatagat ggaatgagta tcacaacagg
     7081 aaagcctgta tatacagatg atttatcagc aaaagatgct ttggtggtaa agatacttag
     7141 aagtcctcac gcttatgcaa aaataaaaga tatagataca tcaaaggcag aaatggttga
     7201 tggtgttgaa tgtgttttaa catataaaga tgtaccaaaa tctcgattta cacttgctgg
     7261 tcaaacatat ccagagccat ctccatatga tagattaatt cttgaagatg ttgtaagata
     7321 tgtaggtgac gaagtagcta tagtggcagc tatagatgaa aaaactgcaa ttaaagcaat
     7381 gaatcttatc aaagtaaagt atgaagtact tacacctgtt ttggattttg aagaggcaat
     7441 aggaaataaa actatggtac atgatgaaga tagacattgt aactttgata taggtatgca
     7501 aagagaaaaa aatatagtat cccatcatga ttatacaaaa ggtgatgtag aaaaagtatt
     7561 aagtaattgt gatgtagtga ttgaagaaac ttattatact caagctcaag cacatgcaat
     7621 gatggagacg tatagggcat acaattattt agaccatatg ggaagattgg ttgtagtaag
     7681 ttcaactcaa ataccatttc atgtaaaaag acatttatct cgtgcactag aaataccagc
     7741 tagcaaaatt agggttataa aacctagaat tggtggcgga tttggtggta aacaaactgc
     7801 atgtgttgag attttttctg caattgttac tttaaaaaca gggaaaccag ctaaaataat
     7861 ttatgataga aaagaaactt ttaattgttc aacaagtaga catgcaatga gattaaaagt
     7921 tagactaggt gctacaaatg atggcataat aaaggcaata gatataaaag cactttctga
     7981 tacaggagca tatggagagc atgcttctac aactattggt ttggttggag aaaaaacttt
     8041 accaatgtat aataaagttg aagcaacaag atttattgca gatgtggtat atactaataa
     8101 gatgccagca ggagcactta gagggtatgg agcatcacaa ggatgttttg ctgttgaatc
     8161 aactataaat atgcttgcta ataaattaaa aatggaccca acagatttaa ggttaaaaaa
     8221 tgtggtaaca gaaggtgaaa ctacatttgc atatggtaaa actttaaata gtgtcgattt
     8281 aataaaatgt ataaataaag gcaaagaact tattggatgg aatgaaaaat atccttgtaa
     8341 acatcttgat aatggaaaaa ttagaagtgt gggtatggca attactatgc aaggttctgg
     8401 tatacaaggt cttgataagt cttcagctga aataaaattg aatgactgtg gagattatac
     8461 tcttatgata ggttctactg atatgggtac aggaagtgat acaattttag ctcaaatggc
     8521 tgctgaggtt cttgatacaa gcattgaaaa tataacagtt atatcagctg atactgactt
     8581 agttccatat gacccaggtt catatgcttc aagtactact tatgtaacag gtatggcagt
     8641 agttaaggca gctcaagact taagagaaaa aataatttct gctggagcaa ctaaactagg
     8701 tgttgagaag gatgaagtag aatttgatgg tataaaatta actagtaaaa aaggtagcac
     8761 tttattattc gatatagcag ttgattctac aattggtcca gatactaatc aattaatagg
     8821 tcttgcttct catggtagcc cagtatctcc tccacctttt atagcaggtt ttgcagaaac
     8881 tgaaattgac acagaaactg gaaaagttga tttagtaaat tatgtagctg tagtagattg
     8941 tggaacggtt ataaataaaa atctagctaa gatacaagtt gaaggtggaa ttgttcaagg
     9001 gattggaatg gcattatttg aagaagttag atacacagaa aaaggaagca tggacacaaa
     9061 tacatttatg caatacaaga ttccagctag atgtgatatt ggaaatgtta aagtagattt
     9121 tgttgaaact tatgaaccaa ctggaccatt tggtgctaaa tctgttggag aagttgttat
     9181 aaatacacct ttaccagcaa tacaagaagc tgtgtttaat ggaattggtg taagaataaa
     9241 tacactccct attacacctg aaaaagtttt tatggaaatg aataaataat tatttatgaa
     9301 aatttagact agttttacat gttaaacaac cagttatgtc atacctgttg aaagatattt
     9361 aataagtagt tattataata ttatgaattt tacttataaa atacaaaatt aaataagggg
     9421 atgatgtttt ggctggtaat ctaaataata tgagagcagt aaataatttt agaggagata
     9481 agaacatttt agaatgttta gtcagctttg agggtcgttc aataagtcag agaaaagtaa
     9541 gggtattttt taaagaaaaa caaaatcaaa tagaaattga ttttgcagaa gaggaaattt
     9601 ctaaattggt tgaaaatgtt gttttaaata catcatatca agaaatgtta tatgatgaaa
     9661 tagagaaaca actggaaatt gattgtatag gtacttggat gatattatct aaattaaaag
     9721 atggtagtag agttcactga ttttaaaata aattattaga gcctaatttt aaggctcttt
     9781 tttgctgttt ttaattaaca atagaaaatt tatctttagt ttgtatataa ttttaatatc
     9841 atatattcta tatatataaa attgaatttg tatgtattat attgtataat ataaaatgaa
     9901 ttgggaaaat agttcgctaa aataatttgt ttttataaaa ttactgttta agtttataga
     9961 ttttgtataa aataaagtta tgttattaat ttttttaaaa ataaaacttt tactaggaga
    10021 cagatatgag aaaaaatagt attaatatag cactaactgc agcaattaca gcaatctatg
    10081 cagttttaac attatcattg ggatttataa gttatggacc aatacaattt agggtagcag
    10141 agattatgat gcttttggca tttttagata aaggatatat agtaggtctt acattaggtt
    10201 gttttttagc aaatgtaata ggaccatatg gtgtacctga tataattttt ggaacattag
    10261 ccacatttat aagtgcaagt atgatttata ttactagaaa gttaggtaaa caaaataaaa
    10321 gtactttagt tatagcttct ttatggccaa ctgttataaa ttcgattgtg gttggtctaa
    10381 tgttaaatgt attctttggg cttccattaa ttctatctat gatacaggta ggttttggag
    10441 aatttgtagt agtaacaatt gtaggagtac catttttctg ctttttaatg aaaaaatata
    10501 aaaatgtcat taatattaaa ttttaaatag tgtatagtaa tttaattaat tgagagtatt
    10561 attagtgaaa agaggtgttt atttgtcgaa ggtaaaaaga ttgactgatg tttggatata
    10621 tattaatgat aagcaaaagt ttacaataaa agagttgtca gaggaattta atctttcagc
    10681 taaaacaata caacgatatt taaaagaatt aaataaaatg ggacttccaa ttcaagcaga
    10741 acaaggtaga aatggaggtt atagggtgct aaataatagc tatatacctc ctgttatatt
    10801 tacagaaaaa gaagttatga ttatactatt tgcacttaag tcacttcaga tttataatta
    10861 taagttaatg gaaatagaaa ttaattctat aatgagaaaa gtaagttatg aacgtaaaag
    10921 cagtataaaa gtaggtatag aaaatattaa aagatacatt ggctttgtag ctaatgatac
    10981 taatgatgga gattttaaga gtagtaatgt aactgagttt tttagagctt cttcagaatc
    11041 tctaatttta aatattaagt ataagtgtgg taatcagatt ttggaaaaaa acgtatgccc
    11101 tataggtata tatttaaata agggagcatg gttttcacca gcatatgatt gtaaaagtga
    11161 taaaataata ttagttcata tagataatat aattgatata aaaagaacag gagaaagtaa
    11221 aaaaatacat ataaacttgg aagaatggtt acatgaagta tataacaatt tatatggaaa
    11281 tagttcaact actaaaaaat ttggtgaaat tatttatcta aatgtattat taactaaaga
    11341 aggtgtttct aaaataaagg acagttcttg gaatttagat atggagctaa atgaagatgg
    11401 tagtggaata attagtacat tcataaaggt tgaagatata gattgttatg cttcgatttt
    11461 attttttatt ggaaataatg ctaaggttat agagccaaaa tttttgaata agctacttta
    11521 tgataaggcc aatgagttaa gatattttta tgaagaaaat atggtataat atttagaaat
    11581 gttgaaatta aataattatt aaggagatat acgttataat ggaaaatttt aataagaatg
    11641 ctaaaataat tggtattgga gctgaaggta ttaatataat aaatgaagta gaagaaaaaa
    11701 taaaagcaaa tatggatata gaaaaaataa acataaatca agagattgaa aaagaatatg
    11761 ttagaaatct tttagatggt gtagatatat tatttttgat atattcatct gaagataaac
    11821 atattagaga tattataaaa gctgtttcat atatgtcaaa cgaaagaaga gttctatcta
    11881 taggaatgga ttgttcagag aaggaaaata aagaagattt agagcttggg agagagttta
    11941 aaatcaataa tgatagtata tttaaatttg ttgatttaat gaatatcatg gtagagtcta
    12001 tttctgattc ttgcatgata aatatagata ttactgattt aaaagaagct attgtaggag
    12061 ataaaggaat taagtattct tttgaagagt ttgaagatac aaagtcatat agcgagatag
    12121 ctgacatttt atttgacagg atggaatata ttggtgagga atttataagt aagaaaggaa
    12181 ttgtatttgt tgaaggaagt ccagaatttt ctataatgga attgaatgat ttaataagta
    12241 atattcagag caaggtagaa gaaagctatg aagtaatatt ttctttatat ataaaggaaa
    12301 atttaaatgg aaatattaga gtaggcttat tgtataatta gtatatttta gatattaagg
    12361 agagtgtata tgtctgaaaa tataagaggt acagagtatt ggttaatgga agaatttttt
    12421 tatcaggaaa cagacacacc acagattttt agagaagaaa gcataattgc acttggattt
    12481 gatataaaaa taaatttaaa aaaagtattc agtatgggga aaaaatcaga aaagtatatt
    12541 aatgagaatt gtaatgataa agattttata aaaaagttta tgtcgattaa aaaaggagat
    12601 tattttttat ataaagcata tcaaaatata gaagaaaaag ttaaatgtaa tgtgtctatt
    12661 ggcatagttg aagaagattt tgaaaatgga tatgaattaa gtcatatttt agggcataca
    12721 ctaccagtaa aatggatgca taattttgat acagtttcaa attgtaaagt atatacaagt
    12781 gaattttata aattaaataa taatgatatt agttcatatt tagaaattat taagtcacaa
    12841 gataagaaat tattgagaaa aagaatagaa gaaagtgaaa ataataaatg gtatttaaaa
    12901 aactattatt ataaaaatgt agataagtgt gtagatgatg aagaattatt tattcagccc
    12961 attaatctag aacaattaga aagcctaaaa aatataaaga cagatgatta tattttattg
    13021 tataatagcg agaaagtaat ttgtgaaaat gaccatacaa tgaagagtct atttggcgta
    13081 tgtcaagtta tggaagggtt cgataaagag aaagtaagtg ctttggaggg tggatatttt
    13141 tttcctgtta gatggataga gtatgaaaag gagaatatag acggaaatgc atgggttata
    13201 tttgaaaata atagtatagt tgaaaagtat gtaaaaagat ttactaataa aatgccatta
    13261 aatactatac tttatggacc accaggaact ggtaaaacat acaatacaaa acagatggtt
    13321 tacaatctag taagtgaaac cagtctaaga aacagatact atacaggtta cttagattat
    13381 aaaaataatg tagaattttg tacatttcat caatcatatg gatatgagga atttatagag
    13441 ggccttaagt ctgatggtga aggcaatttt aagccagaag atgggatatt aaaggatata
    13501 tctatagaag catgttataa tgcattgaaa tttgaaaaaa agatgagctt tgagttggaa
    13561 aaagatagct tgactttaga aactataaaa gacaggaaaa agaaattagt attagaaaat
    13621 atagaatata gtgagagttt taattttgaa gaatcagaaa actatgtttt aataatagat
    13681 gaaattaata gagggaatgt atctaaaata tttggtgagc taataacatt attggaagat
    13741 gataaaagac ttactaaatc aaatcaaact attgtcaaac taccatattc aaaggaaaaa
    13801 ttttctctac ctccaaattt gtatatagtt ggaactatga acacatcaga taaatcaata
    13861 gcacttttag atatagcttt aagaagaagg tttaattttg aagaaattat gccagattat
    13921 gatttattgt ctattgtaga tggtatagat attagaaaaa tgctatatac aatcaataaa
    13981 aggatagagt ttttatatga tagagaccat gttattggac aagcttatct acttgctgta
    14041 gaaaatatta atgatatagt agtagtattt agaagtaaaa taataccact tcttcaagag
    14101 tatttttatt atgattcaga aaaagtaggt ttgattttag gaggaattgg aagaaatgaa
    14161 aaagataaat acattgttta taaagaaaaa cttatagctt ctgagttatt taaaaataat
    14221 aatgcctcaa taatggagag taaagttaac tattgtataa aacaaaacat atctagtgag
    14281 gagctaaaaa atatatatga gtaaaaatat atatgttaag gaaacctatg aatggataag
    14341 gataggtaat ggtgaaaatg agcttacaga aattgaatat gaaaaattgc ttaagtatct
    14401 agagaacaat aatgatgtat taaaaagtaa tataatagat attaaataca aaaaacttag
    14461 atttataaac tatgtaggca tcatatgttt tgaaaatgta atacttgaaa tacttcctaa
    14521 attgtcatta agtgataatc ttgtaaaaga tagagagata ctattacaga tgctttctat
    14581 atgtaataaa attccaatta caatgaatga aaaaataaga ttgagtttga aaaattataa
    14641 tctcttaaat ttttttgtta tgtatttcat tgagagtatg caaacacaga tgaaaaaagg
    14701 tatttatttt gaatatataa ataaaataga gaacttaaat gttatgaggg gtaaaatatt
    14761 attaagtaca tatgcaaaag aaaaaggcat ttctccaatg aaaataagat gtgaatatga
    14821 tgagtatagt gaaaataatt tcttgaatca agttcttaaa aaagcatgta tatctatttt
    14881 atgtagaata aatgacaatt caattcaagg caagattaaa aaaattttaa gctattttca
    14941 aaatgtagat ttaatttata tagatagaaa aaaactgttg gattataaat tttataaaaa
    15001 caatgataga tttaaagatt gttatttact agctagactt ctacttttaa atttgtctat
    15061 ggataatagt caaaataatc aagaggcatt ttctatatta tttgaaataa atactttgta
    15121 tgaagaatat ataggaattt taatcaaaag tatatgggac aattcattta gagaaacata
    15181 tatacaagat aagagtaaat ttcttttgaa aaatgaacaa actggtaaga aaaactttaa
    15241 tttaagaccg gatatagttt tatatgactt aaaaaatgaa tatgaaatta taattgacac
    15301 taaatggaaa gctattgaag tagattcaaa tgttttttat aggtcaagtg atatatacca
    15361 gatgtatgca tatataacag catatgaaaa tgcaaaaaga tgtatattat tatatccttg
    15421 tattcaaaaa gataagaatt atagttcctg gaaattatca gaatcattta agggtaagtt
    15481 tatagaagca aaaactgtga ggttagat
//
LOCUS       DAYATN010000093        15264 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.093, whole genome shotgun sequence.
ACCESSION   DAYATN010000093 DAYATN010000000
VERSION     DAYATN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.093"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..2389
                     /gene="priA"
                     /locus_tag="ACGI8A_002609"
     CDS             <1..2389
                     /gene="priA"
                     /locus_tag="ACGI8A_002609"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFH0180600.1"
                     /translation="GHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPI
                     FRKEDLELVYWMKNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKI
                     STLSDKNREIVEKVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEK
                     KVCYISLSLESDKIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSI
                     NSLSKKKLITLEFKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLI
                     HGVTGSGKTEVYMEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKL
                     SEGEKHDVYKAVKAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAI
                     EVARFMALKNNITLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKD
                     ELDKGNRSIFSLKLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDIS
                     LTYHKKSNTGRCHYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLR
                     MDKDTTSKKGALDEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPD
                     FKSFETTFQLITQVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIR
                     KAFGYSPFNNMLSVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSI
                     SKINQNYRWQILFKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            2407..2847
                     /gene="def"
                     /locus_tag="ACGI8A_002610"
     CDS             2407..2847
                     /gene="def"
                     /locus_tag="ACGI8A_002610"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFH0180601.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            2862..3791
                     /gene="fmt"
                     /locus_tag="ACGI8A_002611"
     CDS             2862..3791
                     /gene="fmt"
                     /locus_tag="ACGI8A_002611"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFH0180602.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            3801..4553
                     /locus_tag="ACGI8A_002612"
     CDS             3801..4553
                     /locus_tag="ACGI8A_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HFH0180603.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            4622..5323
                     /locus_tag="ACGI8A_002613"
     CDS             4622..5323
                     /locus_tag="ACGI8A_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFH0180604.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            5385..6710
                     /gene="rsmB"
                     /locus_tag="ACGI8A_002614"
     CDS             5385..6710
                     /gene="rsmB"
                     /locus_tag="ACGI8A_002614"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFH0180605.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            6718..7749
                     /gene="rlmN"
                     /locus_tag="ACGI8A_002615"
     CDS             6718..7749
                     /gene="rlmN"
                     /locus_tag="ACGI8A_002615"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFH0180606.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            7769..8521
                     /locus_tag="ACGI8A_002616"
     CDS             7769..8521
                     /locus_tag="ACGI8A_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFH0180607.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            8538..10553
                     /gene="prkC"
                     /locus_tag="ACGI8A_002617"
     CDS             8538..10553
                     /gene="prkC"
                     /locus_tag="ACGI8A_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861606.1"
                     /note="PrkC, as found in Clostridium difficile, has a
                     kinase domain, two PASTA domains, and a C-terminal
                     Gly-rich repeat region; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing Ser/Thr kinase PrkC"
                     /protein_id="HFH0180608.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKSKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            10661..11398
                     /locus_tag="ACGI8A_002618"
     CDS             10661..11398
                     /locus_tag="ACGI8A_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFH0180609.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            11496..12380
                     /gene="rsgA"
                     /locus_tag="ACGI8A_002619"
     CDS             11496..12380
                     /gene="rsgA"
                     /locus_tag="ACGI8A_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFH0180610.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            12385..13056
                     /gene="rpe"
                     /locus_tag="ACGI8A_002620"
     CDS             12385..13056
                     /gene="rpe"
                     /locus_tag="ACGI8A_002620"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFH0180611.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            13065..13706
                     /locus_tag="ACGI8A_002621"
     CDS             13065..13706
                     /locus_tag="ACGI8A_002621"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFH0180612.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            13837..14466
                     /locus_tag="ACGI8A_002622"
     CDS             13837..14466
                     /locus_tag="ACGI8A_002622"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFH0180613.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            14521..15177
                     /locus_tag="ACGI8A_002623"
     CDS             14521..15177
                     /locus_tag="ACGI8A_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0180614.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT         6153 a         1516 c         2956 g         4639 t
ORIGIN      
        1 tgggcacaga atattagtac catttggaaa aggtaataaa ccaacagaag cctttgtatt
       61 tcaatttaca gatagcttag atgaaaaaat aaaaataaaa gaaattattg atatattaga
      121 tgaaaatccg atatttagaa aagaagattt agagttggtt tattggatga aaaatagata
      181 tttgtgtact tatatagaat gtataaattt gatatatcca aagggataca aattaaataa
      241 ttataaagta gtcctattag gtgaaagttt aagtggatta aatgatattg aattgaagga
      301 aaaaatatca actttaagtg ataaaaatag agaaatagta gagaaagtga ttgatagtaa
      361 aggtaaaatt aaggttgata agttaaaata tataccaaac ttaaatagtt ctttgtacac
      421 aatgaataag aatggaatta taaaattatg ttgggaatat aaaaatcaca aaaatgaaaa
      481 gaaggtatgt tatatatctt tgagtttaga aagtgataag attgacgatt atatagagca
      541 aaataaaata aatgtgggaa gtaaacaaaa agagatatta agttttctta aaaataatga
      601 aaatgtagag ataaatgatt tattggattt attaaatgct tcaaaacaaa gtataaattc
      661 tctaagtaag aaaaagctaa taaccctaga atttaaggat tattatagag agccaaaaag
      721 tatttacaaa tccgtactaa aaagtataaa attaaacaat gagcaacaag aagcagttga
      781 tgagataaag tcgaatatgt ttgtagatga taaaaaacca tacttaattc atggagtaac
      841 tggaagtgga aaaactgaag tttacatgga aataatagaa tttgctctaa atcaaggctt
      901 agacagtata ttcttagtac ctgagatagc attaactcct caaacaatag ataggctaaa
      961 gagtaggttt ggagatttgg ttggagtgtt ccacagtaaa ctatctgaag gagaaaaaca
     1021 tgatgtttac aaagctgtca aagcaggtaa agtaagagtg ttaattggag ctaggtcagc
     1081 tttatttgca ccttttaatt ccttaggctt aataataatt gatgaatgtc atgagtcatc
     1141 atataagtct gagaagaatc caaaatttaa tgctatagaa gttgctagat ttatggcatt
     1201 aaaaaataat ataactctta ttttaggttc agcaacgccc tcaattgaag agtattatag
     1261 ggcaaaatct ggagaataca aacttataaa tataaaaagt agagcaaatg ataagcctct
     1321 tccaaacatt gaagtggtag acatgaaaga tgagttagat aaaggaaata gaagtatatt
     1381 tagtttgaaa ttacaaaaag aaatacgtta tgcgattgaa gaaaataatc aagtaatact
     1441 atttttaaac agaagaggtt atgcaaattt tgtatcttgt agaaagtgtg gatatgtgtt
     1501 tcaatgtgaa aattgtgata tatctttaac ttatcataag aaaagcaata ctggaagatg
     1561 ccactattgt ggatatgaga aagaaattcc aaaagagtgt cctgaatgta aaagtactta
     1621 tgttaagcca tttggagttg gaactcaaaa aattgaagag gaattaaaat atatttttcc
     1681 agatataaaa actttgagaa tggataaaga tacaacttca aaaaaaggtg ctttagatga
     1741 gatactaaat aaatttaaag ataaagaagc agatgtactt attggaacac agatgttaag
     1801 taaagggctg gattttgaga atgtaacttt agtaggtatt ttgtctgcag acatgatatt
     1861 aaattttcca gactttaaaa gttttgaaac tacatttcaa ttgataactc aagtctctgg
     1921 aagagcaggg agagctgata aagagggaaa agtagtactt cagacttatg acactgaaca
     1981 ctatgctatt aagcatgcta ttgaatatga ttatgagggt ttttatgaag atgagataaa
     2041 aataagaaaa gcatttggat attctccttt taataatatg ttaagtgtag ttgttagtgg
     2101 tgaagatgaa agactagtga taaaaaatat aaaaaatatg catgcatcat tgatttattt
     2161 attagaaaaa aggggtataa atgatttagg atttatatta ggtccaaatc catgttcaat
     2221 atcaaaaata aatcaaaatt atagatggca aatactcttt aaagatgaaa atattgaaat
     2281 taatctctta aagggtataa taaaatatat atgtataaca aaacgagatt tgatatttga
     2341 taagaatatc aatgtatcta tagatattaa tccaaacagt gtattataaa tttaggagga
     2401 attataatgg ctttaagaca aatagttcaa ataggagaac cagtattaag aaaaaaatca
     2461 aaaaaggttg aaaaaattga tgaaaagata atacaattat tagatgatat ggcagaaact
     2521 atgtatgatg cagatggtgt tggactggct gcacctcaag ttggaatatt aaaaagagta
     2581 gtagtaatag atattggaga agagcttata gaacttataa atcctgaaat aatagaaaca
     2641 tctggggagc aaatagatga ggaaggatgt ttaagtgttg ttggagaagc gggaaatgtt
     2701 agaagaccaa attatgtaaa ggtcagagct ttaaatagaa atggtgaaac aatagagtta
     2761 gagggagaag aacttttagc tagagcattt tgtcatgaga tagaccactt agatggaatt
     2821 ttgtttgttg acaaaataga aaagtagtag gagttgattt gatgaaaata gtatttatgg
     2881 gaactcctga tatagcagtt ccatgtttac aaaaaataat agatgagaag tatgaaatat
     2941 tgggtgttgt aactcaacca gataaaccaa aaggaagagg taaaaaacta ggtatgagtc
     3001 cagtaaaaga actggcaatt gaaaataata tacctgtata tcaaccagta aaggctagag
     3061 ataaagaatt tatagataaa attaaatctt taaatccaga tgtaatagta gttgtagctt
     3121 ttggacagat acttccaaaa gaaatattag agattcctaa acttggatgt ataaatgttc
     3181 atgtttcttt acttccaaaa tatagaggtg cggcacctat aaattgggta ataataaatg
     3241 gtgaagaaaa gactggtgtt acaactatgt atatggatga agggctagat actggagata
     3301 tgatactaaa gacagaagta aatcttgatg aaaatataac agcaggagaa cttcatgata
     3361 aaatgatgaa tataggcgca gaaactttaa aagaaacatt aagattaatt gaagaaggaa
     3421 atgctccaag agaagtacaa aatcatgaag aattttccta tgcgccaata atgaataaat
     3481 cattaggtaa catagatttt tcaaaaagtg ccagagaaat acacaattta gttagaggag
     3541 taaatccatg gccaagtgcc tatactacct ataatgatgt cataatgaaa gtttggaaaa
     3601 ctaaagtgtt agatgaaaaa agcacaaaag atgtaggtac aataattgat gtaagcaaag
     3661 atgggataaa agtaagtact atagataatg tacttttaat tgaagaaatt cagatgccga
     3721 ataaaaagag aatgttggtt ggtgaatata taaaagggaa tactatagaa actggtttag
     3781 tattgggtta ggtgaaaagt atgacagata taaagaagta tttaacaagt gttgggattt
     3841 tgattttaat attaggaatt ttgataggag ttgttaattt ttttataata agtttaactc
     3901 aaatattctc cttgatgata aatattataa taatatcact aatacttata atactatgtt
     3961 caacagcagt tacttataga gtaattaaag gaaagtatgt taactcaaat ataatgaaaa
     4021 taaattttaa tattgtcagt ggattatttc ctattatatc tttcattgca tcatcatttg
     4081 gtatgtcaaa aagtgatatt agaagaatat atataaagtt aaataatgaa tatatataca
     4141 gtaataaata taattttaat ccagaggata taataatttt aatacctcat tgtattcaag
     4201 agaatagttg taaattaaaa gttactaatg atatagataa ttgtaaagaa tgtggaagat
     4261 gcaacatagg agaattaatt aagctaaagg aaaaaactaa tgtaaaaata tttgtagcta
     4321 ctggaggaac cctagctaga aaaattataa tggataataa acctaaagct gttgtagctg
     4381 ttgcatgtga aagagatttg acatctggaa tacaagatat taagaaaatt cctgttttag
     4441 gtgtatttaa caaaagacct aatggacctt gtgtaaatac taatgtagac attatggata
     4501 ttgaaaaagc tataggcttt ttaacaggaa aaaatatatt agtttgtaat taaactgttt
     4561 ttaagtgaag tgaatattaa gaggatgtat aataaaaata tattttagga gagtgttatt
     4621 tatgtaccct gtatatggcg gtttttgggg atttgaccca acaatggttg tattaattcc
     4681 agctatatta cttactatat atgcacaatt taaagtgagt tcaacaacaa ataagtattt
     4741 aagagtaaat acacgtcgag gatatactgg agagcaaaca gcaagaagag tacttgattc
     4801 aaatggtttg tatgatgtaa agatagaaat ggttagaggg catctaagtg accattatga
     4861 cccaagaaga aaggctgtaa ggttgtcaga agatgtatat tatggaacat ctataacttc
     4921 tgttgcagta gctgcacatg agtgtggtca tgccatacaa catgctaaag gttatgcacc
     4981 tcttcaaata agaagtagtt tagtaccagt agtgaatttt gcttcaagta tatcttggtt
     5041 tttaatattt ttaggattta ttatggctgg tccattttta aaaattggta tactattgtt
     5101 ttcggcttca gtgttattcc aaataataac attgcctgta gaatttaatg cttcaagtag
     5161 agcaatcgtt caacttggga atttagggat tatagatgaa agtgagtcaa gacaaagtag
     5221 aagagtactt tcagcagcag ctttaacata tgttgcagca gcattggttt caatacttca
     5281 attacttaga ttgttactaa tagctcaaag aagaaatgac taaaagtaaa gtgggttgtt
     5341 tagaaacaac ccttttttat gtttaataaa agggagaaat ttatatggat gcaagagaag
     5401 ttggatttaa agtattatgt gatatagaaa aaaatgacaa ttattcaaat attgcaataa
     5461 ataagcattt taaaaattta gaaataagtg atatggatag aggacttgca acagagttaa
     5521 tttatggagt aatagaaaat aaatattatt tagattatat aatcaataaa ctttcaaaaa
     5581 taaaggtaaa aaaaatgtca acttatgtta aaatcttttt aagaatggga acatatcaga
     5641 ttttattttt agatagtata tcagattatg cagcagttaa tgaaactgta aaattatcta
     5701 agaaatatga taaaaaatct tctggtttca taaatgctat tcttagaaat gaaataagag
     5761 ctaaagagac tataatggac ataactgagg aagatagtgt aaaatactta tctataaagt
     5821 attcctataa ctcttggatt ataaaaaact ggatagataa atttggacaa gaatttgctg
     5881 aagatttgtt ggaagcaaat aatgaaaagc ctagtatcta tataagaacg aatacactta
     5941 aaataagtag agaagaactt attgaaaaat taagtgaaat gggtattgta tgtttaaaag
     6001 ttcctatggt agaagaagct attaaagtcg aaaaaatgaa aaatatagaa aaaaatgaat
     6061 tatttaaggc tggtttattt acaattcaag atataagttc tatgatagta gggaaagtaa
     6121 ttaatccaaa agaaggttca tttgttttgg atgtatgtag tgctcctgga gggaaatcta
     6181 ctcacttagc tactttaatg aacaatactg gtcaagtagt agctagagat atttttgagc
     6241 ataaactaaa gcttataaac tctactgtaa atagattggg gcttaaaaat gtgtttgtag
     6301 aaagatttga tgcttctgag attgatgaaa atagtataag taaatttgat tatgttttag
     6361 cagatgttcc ttgctctggt tttggtataa taagacgtaa accagaaatt aagtataaga
     6421 aagaagaaga gttaaaagac atcacttcta tacaaaagaa aattttagaa aatgcatcta
     6481 aatatgtcaa agttggtgga actttagtat atagtacatg tacaattcaa gatgtggaga
     6541 atataaatat agttacttct tttgttgaag aaaatcacaa ctttgaattt gttccaattg
     6601 atacagtaaa tgtagactta gacaatcaag ataaaggata tttaaagata tatcctaata
     6661 ttcatggtat agatggtttt ttcatagcaa aattaaagag aataaggtag tgagagaatg
     6721 gaaaagaaaa agatagtatt aaaaaatttt actgaagatg aacttaaaga gtttatgaaa
     6781 actatagatg aaaagccctt tagaggaagt caaatattct cttggatata taagggtgca
     6841 aaaacttttg atgatatgaa caatatacca aaaagtttga gaaataaatt agaagaggtt
     6901 tcatgtatag ggcatataga tatagaatta aagttagaat ctaaggtaga taatacaaaa
     6961 aaatatttgt ttttattaga tgatggaaat ataatagaaa ctgtgatgat ggattatgat
     7021 agtagagtta ctgtctgtgt ttctaaccaa gtaggttgta ggatgggatg taatttttgt
     7081 gcatctacaa tggatggatt aattagaaac ttagagccat gggaaatttt agaccaagtt
     7141 attaaaattc aagaagatac tggtaagagg gtatcaaatc ttgtattgat ggggagtggt
     7201 gagccacttg ataactttga aaatacaaaa caatttttaa aaataattaa cgaaaaaaat
     7261 ggtcttaata tagggtatag acatataact ctttcaactt gtggaatagt acctaaaatg
     7321 tatgagttgg cagacttaga aatagctata aatttagctt tatcacttca ttcaccatat
     7381 gatgaagaac gaagaaaaat tatgcctgta gcaaatgctt attctattga agaaatattg
     7441 aatgcatgta gatattatat aaaaaagaca aatagaaggg ttacatttga atattctctt
     7501 ataaaaggag taaatgactc agaaaaagaa gcaaaggcat tagctaagct cctaaaaggt
     7561 atgctatgtc atgtaaattt aattccaata aataaagttg aagaaagaga atacgaaaaa
     7621 ccagataaag catttattta taaatttagg gatagtttgg agaagaacaa tatacctgcc
     7681 acagttagga tgtctatggg ttctgatatt agtggagctt gtgggcaatt gagaagaaaa
     7741 tacaagtaat ttgtaaggag gaagtcgtat ggtttatagt tgtgcctccc atataggaaa
     7801 aataagaaaa aacaatgaag actattgtga gggagaagtt atagatacag aacatggtcc
     7861 aataggaata tttgccatag ctgatggaat gggtggacat aaaaaaggtg aagtcgccag
     7921 taaattagca gtagaaaata taattgattt tctaaaagaa aacttattac aacatgataa
     7981 tgtaaaaata gactatatag atgatatatt aaaacaggct tataataatg taaactcaat
     8041 tgtgcacaaa aaatctatgg aagatataga atttgaagga atgggaacta cattagttac
     8101 ggctatagtg tataataatg tcttatatgt ggcaaatgtt ggagatagca gatgttattt
     8161 gttaacagat gaaaaatttg ataaaataac tatagaccat tccgtggttg aagaacttat
     8221 gatggcccat gtcattacag aagaagaggc aagaagacat ccacagagga atagaataac
     8281 tagagcaatt ggaacagatg atatggttgt tgtagatatt tttaagaaag aaatcaaaaa
     8341 aagtgatata atacttcttg cgacagatgg attaactggg tttattgatg atgaagatat
     8401 aagagattta atattagatt atgaatatga gagaactagt aatataagtg aagaattgat
     8461 aagtatggcc aatgatgttt ctggtaaaga taatgtttcg gttatagtga taaaagtata
     8521 attaggacgg tgatattgtg ggagatacaa ttttaggaaa tcgatatgaa atcatcagga
     8581 agattggtga tggaggaatg gcctttgtat atgaggctaa agacagatta ttaaacagga
     8641 ctgttgcact taaagtactt agacctgaat ttgtagatga tgatgaattt ctaacaaagt
     8701 ttaaaagaga ggcagaagca gtagcaagtc tttcacatcc aaatatagta aatgtttatg
     8761 atgttggtga agatggaaaa gttcactaca ttgtaatgga gtttgtagat gggaagaatc
     8821 taaaagagat tatacaagat gaaggtatat tagatgaata tactgcatta gatataacaa
     8881 aacaaatagc tatggcactt agtgcagcgc acaaaaaggg gattatacat agagatataa
     8941 aacctcataa tattctcata tcaaatgaag gaagagtagt aaaagtggct gattttggta
     9001 tagccaaagc tgtttcaaat tcaacaatga ctaatattgg tagtataata ggttcagtgc
     9061 attatttttc accagagcaa gctaaaagta aatttgtaac taataatgct gacctgtatt
     9121 ctctaggtat agttttatat gaaatgttga taggaaaagt tccatttagg ggagatagtc
     9181 caatttctat agcacttcaa cacattaatg atgatataga ctttacatca gaagaaaaag
     9241 ttaggattcc tcaaagtgta agaactacaa taaaaaagct tacagaaaaa tctagtgcag
     9301 atagatatca aactgctgaa gaattgatag aagatataga gtatatagaa aaaaatattg
     9361 atttagattt tataaaagaa tatgatgatt ttgcaactaa gaaaattgat gaaaaagaaa
     9421 ttaataaagt tgtaaatcca acacttgcaa aaccagctcc tgaaaaagtg gttaagcctg
     9481 ttgaagtagc tgatttggat gatgatgaag attactatga tgatttttat gaagaagatg
     9541 atgatgaaga tgaagaagag gaagagataa tgagagctaa aaagaatcag agacctaaga
     9601 gtactccaag taagagaact aagaaaaaga agaaaaaaca ggaaagccca aaatctagaa
     9661 gaagattaaa agtaattgcg gctgttttga tactgatatt gtgtgctcaa gtattcttag
     9721 catataagtt tttatttgct ggagggtttg gcaataaatc tttgactgtt cctaatcttg
     9781 taaatatgac attagaagaa gctcaaagtg ctgttgaaaa agaaggatta tacctgagtg
     9841 taaaaagtga agagtataat agtgaagttg atgaaaattg tataatatca cagacacctg
     9901 aaggtggaag tacaaatgtc aaaaagggtg atactataaa tgttgtcgta agtaaaggtt
     9961 caagtcaggc atctgttcct aatgtagtgg gacttacttt gagtaatgca aaacaactta
    10021 ttgaagaaaa taacttaaaa gtgggaactg ttaagtatga atacagctca atatataaag
    10081 aaggtacagt tttaagtcag agtccgggtg ctggctcatc aagagctcag gaaggtgatg
    10141 aggtaaacct atatgtaagc aaaggttcag aaaaatcaaa tacacagact ccaacagtac
    10201 cagataaaaa accaacaaca ccagagaata atacaccaac agaacctggt tcaaactcag
    10261 gaaacagtgg tggaaattca ggaggctcaa attcaggaaa tagtggtgga aactcaggca
    10321 ataatggaag taactcagga ggttcaaact caggaaatag tggtggaaac tcaggaggct
    10381 caaactcagg agataatgga ggaagttcag gaggctcaaa ctcaggagac aatggaggaa
    10441 gttcaggagg ttcaaactca ggagacaatg gagatagttc aggaggctca aactcaggag
    10501 ataatggagg aacttcatca ggaactggcg caaacattgg taaaacagaa taaatattaa
    10561 taaaataaga gctgtccttt tatagaaaat attttcttta aagggcagtt tgttttgtat
    10621 aaaatactga attggttata atataaggag aaaagtattt atgaagaaga aaacagtttt
    10681 aattacagga ggagctagag ggattggtaa agctatgtct aaagcttttg caaaagaagg
    10741 ttacaatgta ttagtaaatt ttaataagtc agaaaatgaa gctaaagagt tgtacactat
    10801 attaaatgaa aaaaattttt ctgtaaaact ttttaaggct gatatttcaa atagagaaga
    10861 tgtagaggat atggtagatt attgtataaa agaatttgga ggattggatg tacttgtaaa
    10921 caatgctggt gtaagtcaag ataagttgtt tacagatata actgatgaag attgggataa
    10981 tatgatgaat attaatttaa aaggaagctt ttattgttca caagttgctt taaaatacat
    11041 gatatcagaa aaaaaaggaa atataattaa tatatcttca atctggggta tatcaggtgc
    11101 gtcttgtgaa gtgcattatt ctataacaaa agcaggcata ataggtatga caaaggcatt
    11161 agctaaagaa gtaggaccat caaatattag agtaaatagc atagctccag gtgtcattaa
    11221 tactgacatg ttatctggat ataatgagga agatatagac gctcttgtgg aagaaacccc
    11281 tctaatgagg ctaggaactc cagaagatat agcaaattgt gctattttct tggcatcaga
    11341 caaatcaaat tttatcactg gtcaggtaat tagtccaaac ggaggatttg ttatttaaaa
    11401 aaaatgtggt attatataaa aataggaaag aataacacga tatactactc ataaacagta
    11461 tgtgtttata taaattttat aaagggaggc atttgatgct agaaggaaaa attataaagg
    11521 ggattagtgg attttattat gtagatactt ataatggtat ttatgaatgt aaggctagag
    11581 ggatatttag aaagcagaaa ataacacctt tggttggtga tagagtcaaa ataagcattg
    11641 ttgatgaaaa tgaaaaaaag ggtatcttag aagaaatcga cagtagagat acagaactta
    11701 taagacctcc tattgcaaat gtagataaag cattaatagt attcgcaata aaaaatccta
    11761 aaccaaactt atctctttta gatagattta tagttttggc agaaaaagaa aatttagaaa
    11821 ctgtaataat ccttacaaaa gcagatttag atgataatga tacattagaa actgttaaaa
    11881 atatatatga acttagtggc tataaagtca ttcctgtaag caatattact aagttaaata
    11941 tagataaagt aaaagaagaa ttaaaagaaa atgtagtagt gtttgcagga ccatctggag
    12001 ttggaaaatc aagcttatta aatgaaatag atgaaaattt taaacttcaa acaggagttg
    12061 taagtgataa aataaaaagg ggaaaacata ctactcgtca cgctgaactt ttaaaattag
    12121 aatttggtgg aatggttgca gatacaccag gttttagttc ccttgccctt gaagatatag
    12181 aggaagttga acttaaagat tattttatag aatttgataa atttaatgat tgtaagtttg
    12241 gttcaaagtg tattcatgaa aatgaaccta attgtgctat aaaagaagct gttacaaatg
    12301 gagaaatatc taaagaaaga tatgatagtt atatacagct gctacatgaa ataagacaaa
    12361 ataatagtag gaggtattag aaacatgatt aaattagcac catcaatatt atcagcagat
    12421 tttgcaaaat tattagaaga tgtaagaaaa gtagaaagtg caggatgtga atatcttcat
    12481 atcgatgtaa tggatggaca ctttgtacca aacattacat taggaccact tgtagtcaaa
    12541 agtttaaaga aagaaaatat aaatatggta tttgatgctc atcttatgat agaaaatcca
    12601 gaccagtata ttgaagaatt tgtaaaagca ggatgtgata taataactgt tcaccaggag
    12661 gcttgtgttc atctacatag aactatacaa aatataaaat cacatggaat aaaagctggt
    12721 gtagtactta atccagctac accagtagat acgattaaac atgtactacc tgatttagat
    12781 atggtattat taatgtctgt taaccctgga tttggaggac aatcatttat tccttgtgtt
    12841 ttagataaga ttaaagagtt aaaagctatt atagatagtc aaggattaaa cattgatata
    12901 gaagttgatg gtggtataag ccctaaaaac gtagccgaag ttgtacaagc aggagctaat
    12961 gtaatagtag caggttctgc aatatttgga agtgatgaca ttcaagaaac agttaattta
    13021 tttagaaaga atgcttctct tgaagaatta gtttaaattt aataatgaaa atctgtattg
    13081 ttttaaatgg cgaaattgaa gattacgaag ttacaagaga tattataatt aaggaatgtt
    13141 atgactgcat aatatgtgca gatggagggg caaatcacac ttataaaatg gaaataatgc
    13201 cagattatat attaggagat ttagattcag tagaagaaga aaagataaac ttctataaaa
    13261 ataaaggtgt taaatttgaa aaatttccat ctaaaaaaga tgagactgat acagagcttt
    13321 gtcttttttt agctaagaca cttaaagcaa atcatattga tttttttggt gctttagggg
    13381 gaaggattga ccacactctt gcaaatataa agctattata ttatttaaaa gaagatggta
    13441 tatattctag gatactttct gataaagaag agatgtatat agttgaaaat gaggaaattt
    13501 cattgtatgg aaacccaggg gacactattt ctgtaatagc tataaatggt gatgctaagg
    13561 gtgtaacact tactggctta gaataccctc tcgataatta ttatatgaaa tactctgtac
    13621 ctattggaat atcaaatgtt atgttgagta attcttgtaa gattaaagta gagcaaggct
    13681 gtgtacttgt agttagaaat ttataaaatt tgattttgac aattggtttt atattgtaga
    13741 gcagatacaa tcaagtaaaa tatatttatg attgtatctg ctttttagta tataattata
    13801 aatatataat aaactttgac ataaggggga ttttttgtgg tagatagtat tatttttgat
    13861 ttagatggaa cattgtggga ttcaactgaa gaagtatgca aagtctggca ggatgtttta
    13921 agtgagcata aggaaataga attaagtgtc acaaaagatt tatttagaag tcttatgggg
    13981 ctttcttttg aggaaatagc aaatcgttta tttccaaatt taagtgaaga agaaagaatg
    14041 aatttattga atgaatgttc tttgaaagaa tgtgactatt tatctgaaaa tggagctaaa
    14101 ttatttgatg atatagaaga tactcttaaa ttactatcta aaaaatataa attatttata
    14161 gtaagtaatt gtcaagctgg atatatagaa tcttttttaa aagcacataa actagaacag
    14221 tattttatag attttgaatg tcctggtaat actggtttgc ataaaggaga aaataataaa
    14281 cttataattg aaagaaataa attaataaat ccaatctatg ttggtgatac gcaaggtgat
    14341 gcaaatagtg ctaagtttgt aggtatacct tttatatatg ccaaatatgg gtttgggaat
    14401 gtagatgtat atgattattt tatagattca tttaaagatt tgttagaaca tgatatatta
    14461 aaataagatg agtttaaaaa ttatattaaa ataataaaaa agttatgggg ggatttaaaa
    14521 atggaattac aagatactat ttttaaaaga caaagtgtaa gaaagtttaa aaatcaagat
    14581 gtatcagatg aagatatttt aaaaatgata aaagcagcag gtgcagcacc atcaggcaag
    14641 aatattcaaa actggcattt tgttgttata aaacgccgtg atttaatgga aaagatagca
    14701 gatgtgataa ctaaaaagca acaagaaata cttgtagaaa tggataaagt atcagtggat
    14761 aaggctaata gatttagaaa atttgttaaa aattttacac tattttactt aaaagctcca
    14821 gttttagtat tagtttttac aaaggtatat aatccatcag gatattatga attagagctt
    14881 atagatgcac ctaaggagac tatagataaa ttatttataa gaaatccagg tatgcagagt
    14941 ctaggtgctg caattgaaaa ctttacacta tcagcaattg aacttggata tggttcttgt
    15001 tggttgacaa gtcaaaacta tgcagcagat gagatagaag ctgttttaga agcagaaact
    15061 ggttttgaaa agggagagta ttttttaggt gcaatgttag ctcttggagt gccagaggac
    15121 aatttgaaga gtccatctaa aaaaccagta gaagaaatat gtacatttat aaaataaaag
    15181 tcaaggacat tttaaaatcg aagtaaatta aaaacaaaat aaattaaaaa taaatattta
    15241 aaaaattaaa aagtaggttt tatt
//
LOCUS       DAYATN010000094        15205 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.094, whole genome shotgun sequence.
ACCESSION   DAYATN010000094 DAYATN010000000
VERSION     DAYATN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.094"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            332..913
                     /locus_tag="ACGI8A_002624"
     CDS             332..913
                     /locus_tag="ACGI8A_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFH0180615.1"
                     /translation="MMGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLD
                     TAAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPG
                     EISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVA
                     EIYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            952..1530
                     /locus_tag="ACGI8A_002625"
     CDS             952..1530
                     /locus_tag="ACGI8A_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFH0180616.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1616..2245
                     /locus_tag="ACGI8A_002626"
     CDS             1616..2245
                     /locus_tag="ACGI8A_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFH0180617.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2235..4994
                     /locus_tag="ACGI8A_002627"
     CDS             2235..4994
                     /locus_tag="ACGI8A_002627"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFH0180618.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            5081..6643
                     /locus_tag="ACGI8A_002628"
     CDS             5081..6643
                     /locus_tag="ACGI8A_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HFH0180619.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6644..7780
                     /locus_tag="ACGI8A_002629"
     CDS             6644..7780
                     /locus_tag="ACGI8A_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFH0180620.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7844..8920
                     /locus_tag="ACGI8A_002630"
     CDS             7844..8920
                     /locus_tag="ACGI8A_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFH0180621.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8898..10136
                     /locus_tag="ACGI8A_002631"
     CDS             8898..10136
                     /locus_tag="ACGI8A_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFH0180622.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10190..11278
                     /locus_tag="ACGI8A_002632"
     CDS             10190..11278
                     /locus_tag="ACGI8A_002632"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFH0180623.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11306..12097
                     /locus_tag="ACGI8A_002633"
     CDS             11306..12097
                     /locus_tag="ACGI8A_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFH0180624.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12212..12766)
                     /locus_tag="ACGI8A_002634"
     CDS             complement(12212..12766)
                     /locus_tag="ACGI8A_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFH0180625.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12962..14164
                     /locus_tag="ACGI8A_002635"
     CDS             12962..14164
                     /locus_tag="ACGI8A_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFH0180626.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14221..15201
                     /locus_tag="ACGI8A_002636"
     CDS             14221..15201
                     /locus_tag="ACGI8A_002636"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HFH0180627.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
BASE COUNT         5972 a         1579 c         2775 g         4879 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaaat attaaacata gcttttatac atttaataag gtatgtaaaa tctatgtaaa
      181 aagtaagaaa tataaacatt tttttaatta tattttaaaa atatcaagca agttttcttc
      241 acaaatgaat aaaaaatgtt ataatatgat taaagaaaaa gataaataat tagcaagatt
      301 tgtttagcta aaattaacaa gggagcgata aattatgggt ataacattat caaaaggcca
      361 aaaggttgat ttaactaaag gaaatccagg tcttaaaaat attttagtag gtttaggatg
      421 ggatactaac aagtatgatg gtggatttga ctttgattta gatacagcag catttttaac
      481 aggagcaagt ggaagtgtta ctaatgatgg agattttgta ttttacaaca atttaaaaca
      541 tacatctgga gcagttgagc atctaggaga taacagaact ggcgaaggtg atggagatga
      601 tgaacaaata gttgttgatt tatctaagat tcctggagaa ataagcaaga tttcttttac
      661 tgttacaatt catgatgcaa cagaaagaag acaaaatttt ggacaagtaa gtaattctta
      721 tataagaata gtagataaag acacaaatga agagttaata aagtatgaat taggagaaga
      781 ttttagtata gaaacagcta tagtagtagc tgaaatatat aagcacaatg gagaatggaa
      841 gtttaatgca ctaggttctg gctttgaagg tggtcttgct gcattatgtg gaaactttgg
      901 aataaactta taatttcaac tataacaata aactaaaggg ggatattttc catggctata
      961 gtattaaaaa aaggacaaaa aatcgattta actaagggaa atccaggtct taaaaatata
     1021 aaacttggac taggatggga tacaaattca tttgatagtg gctatgatta tgatttagat
     1081 gtaagtgttt tcatggttgg agaatctcaa agagttgaaa aagatgaaga ttttatattc
     1141 tacaataact taaaacatcc atctggagca gttgaacact taggtgataa tagaactggt
     1201 gaaggtgatg gagatgatga ggaaatatta gtgaatttag agacaatgcc aaagcatata
     1261 caaaagatag ctgtaacagt tacaatatat gaggctgctg aaagaagaca aaattttgga
     1321 caagtaagta actcatatat aagagtatta aattctgaaa atgatgaaga aatattaaga
     1381 tatgacttag gtgaagaatt tagtatagaa actgcaatta cagtatgtga gatatataag
     1441 tataatggag aatggaagtt tagtgcagtt ggagcaggat ttgaaggtgg cttagaagca
     1501 ctttgtagaa actatggctt aaatgtttaa tttaagcaat ataatatatt aaatataaat
     1561 aacctataca aataatgtat aggttatatt atataaaatt ttgtggaggt ttagtatgtc
     1621 gattaattta tcaaaaggtg ataaaataga tttaaaaaaa tctaacccag gactgagtaa
     1681 catacttgta ggtctaggat gggaccctgt gcaacaatct ggtggaggtt tttttaaatc
     1741 gttatttggt ggaggacaag ctgatataga ttgtgatgca tctgtattta tgcttaatca
     1801 agaaggtaaa ttaagtggaa taaaagattt aatttatttt ggaaatctaa aaagtgcctg
     1861 taaatcagtt ttgcatacag gagacaatct aactggtgag ggtacaggtg atgatgaaca
     1921 aatcctagtt aatttggata aagttccatc aaatattcat aaattattgt ttgtagtaaa
     1981 tatatacaat tgtgtagata gaaaacaaca ctttggaatg atagaaaatg cctatataag
     2041 agttgaagac caaggtaata agaaagagat agcaaaatac aatctatctg ataattattc
     2101 tgaaaaaact actcttatag taggagctat atatagaaaa gatggaagtt ggcaatttaa
     2161 agcaattggt gaaggaacaa aagatgcggg tcttaaagaa gtaatgcaaa atttagacag
     2221 gattgagtgt gcatatggaa tttaataatg atatcaaata ttataatgta gataaagaag
     2281 aagttacaaa agacttatct gttaatcctg aaaaaggttt atcagaatca gaagtcaaga
     2341 ctagaagaga aaaatatggt ttgaatgaat ttactccaaa agaagagggt agtttttggg
     2401 atgatttaaa ggaaagttta agtgaaccaa tgatagttat cttgattatt gcagcagtag
     2461 taagtgcagt aattggggaa actcatgatg caatagggat tgtaggtgct atagcaatag
     2521 gtatagcaat aggtatgatt acagaaggtc gttctaagaa agcagcagag gctctttcta
     2581 agttgactga aaacatagaa gtaaaagtat tgagagatgg ggaagtacat caaatttcta
     2641 agagtgaatt ggtaccaggt gatatagtat atattgaaac tggtgacatg gtacctgctg
     2701 atggtagact tatagaaagt ataaacctta aaattagaga agatatgtta acaggagaat
     2761 ctgatgacgt atctaaaaag tgtgatgtag tagtttctat ggaagagata gaatctaaag
     2821 gagcagtagt agtacaggag ccaatacctg caaaacagat aaatatggtg tttggtggca
     2881 cacttgtagc ttatggtaga ggagctctag ttgtaacttc aattggagat aatagtgaaa
     2941 tgggtaaaat tgctcaaaac ttatcagaaa cagatgagca aactccactt cagataaaac
     3001 ttggaaattt aggtggtttg atagccaaag tttctagtgc agtagcagga ttactattta
     3061 tatttatggt tttccaaatg ataagtaaga atgtattaaa tgtagatatg tctggaatat
     3121 taccgttttt agaatcaata gacccagtta aaacagcatt tacagtatgt gttgcgttga
     3181 tagttgcagc agtgccagaa ggtcttccaa ctatgataaa tatgacactt gcaataacaa
     3241 tgcaaaaaat ggctaaaata aatgcattgg ttactaaaaa agaagcttgt gaaacgatag
     3301 gttctgtttc tgtaatatgt tcagataaaa ctggtacatt aactgaaaat agaatgactg
     3361 tagaagttgc atatgttgat ggcagatata ttgaaagttc agaagaggaa ataaatagtt
     3421 attttgaaga aaactgtatg ataaactcta cagctgatgt agagcacaat gatggtgata
     3481 ttaagtattt aggaagtgct actgaatgtg cattgctttt atattacaaa aacgtagatt
     3541 atagacaagt tagaaaaaat tctaatatag tagctcaaaa tccttttact tcagattcaa
     3601 agagaatgac ttctgttata ggtcaagata atcatcatgt actattatca aaaggagcac
     3661 cagaagtctt acttgaatta tgttcacata ttcaaagagg aaaagacata gttccattaa
     3721 ctgaaaatat aaaacatgag attttagaag aaataaagaa attgcaaata aaatcaatga
     3781 gaactttagg gtttgcatat aaagaaatct cacaagctga agaagaagca gcagtaactg
     3841 ctgaaagtga tgctatgaat gtaagtgcaa tggaaaacaa tttagtattt agtggttttg
     3901 ttggtataag agacccactt agaaaagatg ttatagagtc tgtaaataca gcaaataaag
     3961 ctggtgtttc tgtaaaaatg cttacaggag ataatataaa tacggcaaga gctataggtg
     4021 aagagttagg cctattaaaa aataatatga gagtggttga agcttcatat atagatactt
     4081 tagatgatga agaattaaaa caagagatac aaagtatttc aatcgtggca agaagtaaac
     4141 cagatagtaa aatgagaata gtttcagctc tgcaaaagag cggagatgtt gttgcagtaa
     4201 caggtgatgg tataaatgat gctccagcac tttcaaaagc agatgttggt atagcaatgg
     4261 gtatatctgg tacagaggta tctaaaaacg cagcagacat aattttaact gatgatagtt
     4321 ttagtaccat tgtaaaaggt attaaatggg gtagaggaat ttatgataac ttccaaagat
     4381 tcgttcaatt ccaattaact gtaaatgtaa ttgcattttt agtggctata atatctcaag
     4441 ttatgggaca agagatgcca tttactacaa tacaattatt atgggtaaac atcattatgg
     4501 atggaccacc agcactagca ttaggtttag agccagttag ggaccatgtg ttaaatagaa
     4561 aacctgttga tagaaaagcc aatataattt ctaagtcaat ggtgtataca atagtattaa
     4621 atgcatttta tataacagca atactaatgt tacaatctac atttaatttc ttaggagttg
     4681 atgaagcgca taagggaaca gtaatgtttg cgttatttgc ttttagtgca ctgtttaatg
     4741 catttaactg tagagagttt aatgcagata gtatcttccc taattttaca cataacaaat
     4801 tagcactaca aataatagtg ttgacaggaa ttgcacaagt aatatttact cagctattcc
     4861 aagatttctt taactctgta gcaatggatt ttatgacatg ggtaaaagtt ttatgtgtag
     4921 cagcaagtgt aattgtggtt aatgaagttg taaaatgtat aataagactg gtaaaactaa
     4981 aaaagactgc ttaaaacaaa ttttttagaa gaaaacagac tgactcaaaa tatgaatttt
     5041 gggtcagttc tgaataaaca tttaaattgg gaggatagtt atgggtgact caaaggtatt
     5101 tgaaaaaata ttctccagcc aatcagatag aggaaattac actccttcaa agggatatct
     5161 aagttatttt atttcgtata ttggtttaga agatgaagtt ttgtacaatc tcgagatatt
     5221 taagacaaaa caaaatattg atagcaaaaa agatattgct ttatttactg atgttatagc
     5281 aaatcctagt gattttgata ttataaatta ttttaaaagt ggacttcaaa aatatagaac
     5341 tagcatggaa gatgtagata taaatatttt gggatttgaa gagattgatt ataaaataaa
     5401 gcaagcaatg gatagagtgt taaaagaaga ggaaaaagaa tttactaatg atagagttaa
     5461 acaaaatttt atagtgaaaa taatggcatg gataaaaatt tatatcggtg ctttagatat
     5521 aaataaaaat gaagctccaa aagtaatatt ttatggagat ataaaaaaac atgaagttta
     5581 tttactctta atattatact tggcaggttt tgatgtttta tatcttaatc caaattcaaa
     5641 gagtaatata gatattttaa aatcagaaag atacaatata gagtttgaag aagccaacat
     5701 aatagaagaa aaaatttctt ttgaagaaag ggttatttta ggggaaaaaa tagataaaag
     5761 ttctgtaaaa aaagctttta ctgttggggc agaagcatca aagcgtataa gtgaggaatt
     5821 gttaaatgat gcaggattta taaaaccttg gcaactccaa gatagaaaaa taaaaaatct
     5881 acttctaagt tcaactgttg atgaaatatc aatatactgg aatcaaccac ttaaattaag
     5941 accaggattt aagtttaatg atgctattgt agaagctcca aattttcttt ctaagattaa
     6001 tggaatatat aacgataaga atgaatatat taagttttta gatttattaa gagattcgga
     6061 aagttcaaca tttattgaat ttaatggaga tgtagataga ttttcaaaag cttttacaag
     6121 agaagctttt agtttatcct ttttattgga ttcaaaaggt gcaatagata aaaactctgt
     6181 tttaaataat aaagattatt ccatatcaac actagcattg aatcaacaaa ttatgatttt
     6241 agagaaagtg gaagagttgt tagaaggaag tatgtttttg aatggtttaa gtggagaaga
     6301 caagataaaa ggtctattta cagtacttca catggataag aaatttgttc atatgatgaa
     6361 caatttcgat tactcactta taaacccaaa attgattata tacatgtaca agagtattgt
     6421 atttgataaa gaaatagttt ttcttatgct tttacttagt aagattggat ttgatattat
     6481 catattatgt ccaggaggag aaaacaatat agagaatgtt ataaataacc aattgattga
     6541 tgtacacaga ttggataaaa tggtatatga ccttaaatta aattcgcttg aaaatgatat
     6601 acctttactt aaaaaaattt ttggaaagag gagacgattt tagatgggga taaatataaa
     6661 tagtttttca aaagataaga actctgtaga ggttactgaa aatttgccta cagcagttca
     6721 agaagaaaac ttcgatataa tggaatatac aaataataaa aaaaatgaac tgagaaagtc
     6781 taaggaagtt gaggcattga catctcttat agaagtagaa aatccagata cgattttaca
     6841 gtttggtagg aaagcatctg aaggagtagc aagagtttct gatagccttt taaatacaat
     6901 aaaattaaat agaaatgaag aaaattctaa aatgttggtt catttaacta aaataatgga
     6961 caagtttgat ttagacgatt ttcaagaaac taaggaacca aatttcgttc aaaaactttt
     7021 caaaaaagct aataatgcca ttgaaatgat gtttcaaaag tatgaaacat tgggaggaga
     7081 agttgagaaa attcaaattg agcttgaaca atacgaaaga gatatagcac tttctaataa
     7141 gcaaataggt gctatgttaa atgaaaactt tgagttttac aatgaacttc aaaaatatat
     7201 tgttgctgga gaaatggcaa tagaagaaat ggataatgaa atactacctt tctttaaaca
     7261 aaaatcagag actagtggag accaaatgga tgttgtcaat tatcaagaac tattaaaagt
     7321 ttatgatatg ttaaatcaaa gagtctatga tttaaggata gcagaaaata tagctatcca
     7381 aacaatacct atgcttagag gtatgcaaca taataattat ggtttaatta gaaaaataaa
     7441 tagtgcattt gtcgtaactt taccagtatt taaacaatgt ttatctcaag ctatattact
     7501 taaaaagcaa gaattacaag caaaatctct aaaagcactt gatgacaaaa ctaatgaatt
     7561 attgcttaga aatgcacaaa atgtaagtac tcaaagtgca caaatagcta gaatggctgg
     7621 aacaagttct gttcaaatag aaacattgga aaaaatgtac aatactatca agtctggtat
     7681 agatgaaact atgagaatag aagaaaacaa tagagcttta attaaggata atacaaagag
     7741 attggaagaa ttaaatacga ctataattta taataagtag tattatatta agggctaatt
     7801 tgtataaagt tggccctttc tatgtatatg aggaggctag acattgaaat atttcgatta
     7861 tatctgcaaa gatgatttgg agagaatttt tttgaaggag ccagaagatt ttagtgctaa
     7921 gactgaaaaa gatgtgttga aatatgcact gggcgctttt ttatacgttc cagctactca
     7981 atataatatg atttataaga gtattatagg ggatgttaag ggagttagac cattagctat
     8041 ttgtttagaa gatgctgttg gagtaaatgg agagttggag gctatagaaa atcttagatt
     8101 aattttaaaa aatattagca atgaaagtat aactaataaa gatggaatac cactaatttt
     8161 tgtaagaata aaagatgtgg agcaactgtt gagaattaaa gagattataa ttaaaaatag
     8221 tcattttata acaggaatat taattcctaa agcaaattcg gagttaattg aaaattgtat
     8281 agaagcttta gattttatga atcttcaaga tatgtatgtt attccgatag tagagacaaa
     8341 agaatttata tataatgaaa aaaaagaact tagttttact aatttgtaca atgctatttt
     8401 aagacataag tcaagaatat taagcattag agtaggcata actgatatat tagggatgta
     8461 tggtataagg agagataaaa acttttctat atataataat ttgatttgtt cttcatttat
     8521 attagatata atcacatatc ttcaaagacc agagttagat atacctataa gtggtggagt
     8581 atcagaattt tttgatatga cgaataagga tataagaaat aagtatatag aagaaatatt
     8641 attggataaa tatcatggat taattggaaa gaccgtaatt catccaatgc aaattcaaat
     8701 tgtacaagcg ctatctacag tatcatatga agattttact gatgctttgg atatactgga
     8761 tagtacagac agtaagtatg gtgtgagcaa gggtgtatta ggagagagaa tgaatgaaac
     8821 aaatccacat tttttatggg ctaaaaaaac attgattcta tctaaaatat atggtgtatt
     8881 aaataaagga gtagactatg aagaattgct taaattttaa ttgtgagatt gacattattg
     8941 ataatccatt gaatttagaa ctaaatgatt ttttagacgt aggaataaga aataatagca
     9001 aaagacgttt tttgtttatc tcaaagaaac tcggtaaaca tttagcatgt aagccaagtg
     9061 agatggataa tttgggaaaa ttaatggcta caatttataa tgaaaaaaat aaggaagaat
     9121 caaatggtac tgttatagct tttgctgaaa ctggtacagc tgttgggcat agtttttttg
     9181 attacttatg tggagattat gaatttattc atactactag agagcagttt gacgagttag
     9241 aaaatctgga atttttagag gaacattccc atgcaacaga ccagaattta tactttggta
     9301 tgcttaacaa ctttaaattt ggagatgaga taatattggt tgatgatgag attaccacag
     9361 ccaatacatg catgaatata attaggaaaa ttcaaagcat ttatcctaaa aagaaataca
     9421 ctatatgctc aatactcaat tgggtaagtg atgaaaattt aagtaaagtt actcgtttag
     9481 aaagagagct agattgtaag atagattttg tatacttatt taaaggaaac tttaaatttt
     9541 atatagatga agatattatt tcaaattaca acgataatta tagtattact aaattagaga
     9601 ctatttcaag tgataaagta aatttagtag taaatcatat ttatgttgat ttagaagatt
     9661 atattggaaa taagaaatat gtaaaatata caggaagatt tggaattaat agaagagaac
     9721 aatttagatt aaaagaaatc gttaagagag aaagtaaaaa aataactatg gccagtaaag
     9781 aatctttaaa aaatgaaaaa gaaaagattc tatttttagg tactgaagaa tttatgtata
     9841 tacctatgct gtttgcaaaa gaaaaagaaa atcaatatga tatatactat cattcaacaa
     9901 caagaagtcc aattattaat attgataaga ataattatcc tattaaaagt aaatttatgc
     9961 taaagagttt ttataatata gatattcaaa attatattta caatatagat aaacataatt
    10021 tttctaagtg ttatttattt gtagagctga ataaaagtga aaattcttat ctagaattta
    10081 ttgatataat taaaaaaaca agtataaaag aattaactat agtatgttgt agctaaagtg
    10141 ttttaacagg ataaaatata tcacgatttt aagataggag ttgagtcata tgagagattt
    10201 tagcacgtat tctaaagatg atgttatttt tttattaaaa gatatatcaa atttaattga
    10261 agaagaagga aataaagaaa gagaattaaa aatacaaaat ggtagacatt attcagaaat
    10321 gataccaata gagtatgaag ttagtgaaga ttatctgaat ttatatcatg agaaactaag
    10381 agaaaataaa gataaacttg cattttctat aggagttatg tgtgaaaaaa taattaaaaa
    10441 aaatggaaat gatgtagtac tagtttcact tgctagagca ggaacaccta ttggtatttt
    10501 ggcaaaaaga tatataagaa gcaagtataa tataagttta cctcactata caatctctat
    10561 aataagagat aagggaatag atatgaatgc aattgaatat atagtaaaga atcatccgag
    10621 ctcaaaaatt caatttttag atggatggac tggtaaggga actatatcaa aagaacttga
    10681 aaaagcatgt gatgaactta atgttaaatt taaaaaatat tttgactcaa cacttgctgt
    10741 attagctgac cctgctggat attcaggact ttatggaatt agagaagatt tcttaatacc
    10801 aagtgcatgt ttaaattcaa cagtttctgg tttagtaagt agaactgtac taagagagga
    10861 tttaattggt aaaaatgact tccatggagc taagttttat agacatttaa aagataaaga
    10921 tgaatctatg aactatatcg aaactataga agaatgtttt aaaaatcaat ttaagaatat
    10981 atctgatgaa gttgaaaact gggaaagtga cattataaca agagatggat actttgatgt
    11041 tttaaatata aaagaaaagt ataacataac agatattaac tttatcaaac caggtgtagg
    11101 ggaaacgaca agggttttac ttagaagagt tccgtataag attcttgtta aagacttaaa
    11161 tgataaatct ttagaccata tattcatatt agctaaggag aaaaatgttg aagttgaaca
    11221 aatggattta aaagcctata aatgttgtgg aataataaaa aatatgaaag atatttagga
    11281 aatatattag acaggagaat attgaatgat tgttttttca gatttagaca ggagtatcat
    11341 ttactccaat aaatttttaa atgctgatag taaatatact aatatagaaa tttataggga
    11401 aaaagaaata tcttatatat ctttagatac tataaattta ataaagcaaa tacaatatta
    11461 tggtatgttt atacctacaa ctactagaac agtggagcaa tttaagcgta tagaatttaa
    11521 taaatatggg atttattttc cttggagtat aacttcaaat ggaggagtta tacttaaaga
    11581 taatgaaata ttgaaatcat ggagtgaaaa aatagataaa ctaaaatgtg attatgagcc
    11641 cattgaaagt atgattcata aatttaaaga ctatttgaat gtagatggaa taactaattt
    11701 taaagttgca gaagatacat ttttttatat agttgttgat ttatcaagat ttaatttgga
    11761 ttcaatcaaa gaatatacaa atatattgga atcaaaaaat tggaagtttt atgtgagtgg
    11821 cagaaaaata tattttatac caaaagaaat ttcaaaagaa aatgctataa aatacttgac
    11881 taaagaatta ggaatagaat atttttatgc agttggagat tctattatgg actatggtat
    11941 gcttaatatc tcaaataaat cctatgtatt gaagcatggt gatataaata aaaatgaaat
    12001 agaaaattca tttatatcaa gttctttcaa tggaatgagt ggaactgaag agatactatc
    12061 taacatttta gacgagaact gcctacttaa tatataatga ctgatatata aataataaac
    12121 tagaaaaatc tgaaataaat tatagtaaag tttatcatac taatttattt cagattttta
    12181 tcaatttata tttaagtaag aactcaatat gttaaaatga aggtggagtc attgcccaga
    12241 ctgagataca tacctcatca tcataatttt catacacatg tggtatagta ctgttaaaat
    12301 aaaaactatc tccttcctcc aatagatatt ccttatttcc aagtgttatc ttcattttac
    12361 cttttattat atagccacat tcttctcctt cgtgtgtcac aaattcactg tttttttgtg
    12421 tatgaccatc tagtgtaaca agtataaatt ctattttttt cccagataaa ttaggaacaa
    12481 gaagttcata aaattttgta gaatcattag ttactatttt ttttcgatta tttttcttta
    12541 ctactatatc agagtcttct cttccaatct cttcaaaaaa ataacctata tttatatcaa
    12601 gagcttttga tattttccac attgctacaa tagatggaac aaccatatta cgctcaattt
    12661 gacttattaa gcctgtactt aaatctgaat tttttgctaa agtagagata cttatatttt
    12721 tagattttct aagagatcta attttgcttc caacatctat attcattaga tacaaacccc
    12781 ctcagacatg attataacat ataatttata aagattaata aaataaaaaa aaagttttac
    12841 aatacaagta ttttttatgc tatattgaat tttctgaaaa gatgcattat aatagtatta
    12901 atattcaata tattgaatat tgataacgat attttaattt aatgaaatgg aggtaattaa
    12961 gatgaagagt tttgtaagtc ctgataatat ttgggtatta tgggcagttg taactggatg
    13021 ggcagcattt agtatttatt tggaacaaaa gtacaattgg gcatcaaaag tgtcgggagc
    13081 aataatagct cttgtaggag caatgttatt atcaaatcta aatattatac cagtagagtc
    13141 tgttgtatat gaccaagtat ggggatatgt tgttccttta gccatagctt tattgttata
    13201 tcaatgtaac attaaaaaga tatggaaaga aagtggaaga ttgctcatta tatttcttgt
    13261 aggttctgta ggaactgtac ttggagctat gattggattt ttagcattaa aaaatgtagt
    13321 accagattta aatattgttg cagctatgat gacaggttct tatataggtg gaaatgtaaa
    13381 ctttgctgct atgtcagggg cgtttgatgc gccaggagaa ttagtatcag caacagttgt
    13441 agcagataat cttttaatgg cattatattt ctttgtctta atagctatac catcaattgg
    13501 attttttaga aaacatttta aacatccaca tgtagatgaa atggaaagta taggtataac
    13561 tgaaggagaa acagtagcag caaattattg gggaagaaaa gaaatatctt taaaagatat
    13621 agcaatagcc attggtagcg cctttataat tgttgcagta tctgtagaac tatcaacttg
    13681 gtttaaagct ataataccat tatcaaatcc ttttttagca atgttaaata ctttatttgg
    13741 aaatcaatat ctaataataa ctactttgac tatgttgttt gccacattta tgcctaattt
    13801 ctttggtgat ataaaaggag cacaggaatt gggtacattt ttaatatata tattctttgt
    13861 tgtagtaggt gttcctgctt caataacatt aataattcaa aaatcaccat tactactttt
    13921 atactgcgga ataatggtac taataaacat gctagttaca tttatagttg caaaaatatt
    13981 taagtttagt ttagaagaag ctattcttgc atctaatgca aatattggtg gtcctacaac
    14041 agcagcagct atggcaatat caaaaggatg gtctaagcta gtagggccaa tacttatagt
    14101 tggaacattt ggatacatag tcggtaatta ttttggtctt ttagtaggaa atattttgat
    14161 ataattttac gtgaaaaaaa gttgtttatt agaaagattt ttatttaatt ggaggttgtt
    14221 atgattttcg atggacattc agatacttgg gcagatgtaa caataagaag gcttaacggg
    14281 gaaaaagatg tatttagaag aatccatctt gataatttta aaagagcaaa tatagaagga
    14341 gctatttttg taatatggat agacccacca tatgataaag agcctgaaaa aagagttaaa
    14401 caaattataa gttctataaa agatgaaatt gaagatatga aagatgttgt aaacatagta
    14461 aaaaaatatt cagatataga taaaggtata agtgaaaaca aaataaatat tttaattggt
    14521 cttgaaggat taagccatat tggaagaaat atagatttac tagattattt ttataaagaa
    14581 gttggtgcaa gacatgcatc attaacttgg aatgaacaga atgaacttgc tacaggatgt
    14641 ttaggggatg aaaatcgtgg tttaagtgaa ataggcaaaa aagcagtcaa taaaatggaa
    14701 aaattgggca tgatagtaga tgtgtcacat ttaaatgaaa aatcattttg ggatttaatg
    14761 tctgttgcaa gtaagccagt tatagcttct cattctaatg ctaggagttt atgtgatgct
    14821 aaaagaaatc ttacagataa tcaattaaaa gaaatttcaa atacaggtgg attagttgga
    14881 ataaatagtt ttagaggatt tactaatagt aatcctaaga agcaagattt aatacatttg
    14941 gttgaccata tagactatat ggtaaatctt ataggtatag aacatgtatc ttttggtttt
    15001 gattttagtg attacttagg gaaagagact ataggttctt ttactggaaa tgctagtgtg
    15061 ccaagtgtag atggtcttga aagcacacat acagcacaga acatagtagt agagcttgaa
    15121 aatagaggtt ataaaaagaa agatatagag ttaatctcta gtggtaattt ttataggctc
    15181 tttaaagata tactaaaata atata
//
LOCUS       DAYATN010000095        15162 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.095, whole genome shotgun sequence.
ACCESSION   DAYATN010000095 DAYATN010000000
VERSION     DAYATN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15162
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.095"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(118..1005)
                     /locus_tag="ACGI8A_002637"
     CDS             complement(118..1005)
                     /locus_tag="ACGI8A_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFH0180628.1"
                     /translation="MAIYLEMPKLIHEFPFRTLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            complement(1105..1554)
                     /locus_tag="ACGI8A_002638"
     CDS             complement(1105..1554)
                     /locus_tag="ACGI8A_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0180629.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            complement(1544..3451)
                     /locus_tag="ACGI8A_002639"
     CDS             complement(1544..3451)
                     /locus_tag="ACGI8A_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0180630.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(3719..5614)
                     /locus_tag="ACGI8A_002640"
     CDS             complement(3719..5614)
                     /locus_tag="ACGI8A_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFH0180631.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(5650..6528)
                     /locus_tag="ACGI8A_002641"
     CDS             complement(5650..6528)
                     /locus_tag="ACGI8A_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFH0180632.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(7071..7241)
                     /locus_tag="ACGI8A_002642"
     CDS             complement(7071..7241)
                     /locus_tag="ACGI8A_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180633.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            complement(7638..7850)
                     /locus_tag="ACGI8A_002643"
     CDS             complement(7638..7850)
                     /locus_tag="ACGI8A_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0180634.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(7851..8267)
                     /locus_tag="ACGI8A_002644"
     CDS             complement(7851..8267)
                     /locus_tag="ACGI8A_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180635.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(8335..8748)
                     /locus_tag="ACGI8A_002645"
     CDS             complement(8335..8748)
                     /locus_tag="ACGI8A_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180636.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(9581..10210)
                     /locus_tag="ACGI8A_002646"
     CDS             complement(9581..10210)
                     /locus_tag="ACGI8A_002646"
                     /EC_number="3.2.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HFH0180637.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(10289..11251)
                     /locus_tag="ACGI8A_002647"
     CDS             complement(10289..11251)
                     /locus_tag="ACGI8A_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0180638.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(12013..>15162)
                     /gene="cbpA"
                     /locus_tag="ACGI8A_002648"
     CDS             complement(12013..>15162)
                     /gene="cbpA"
                     /locus_tag="ACGI8A_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HFH0180639.1"
                     /translation="AGDAEGDANAVVVEEEATAEEPTSVGDFKISGGEINVDFKFASG
                     VLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKTASNSPFTLTPDKDG
                     GAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNRDIEGEFIVPEGGKIP
                     RDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNTESGGNIIINGGAIT
                     AIGQESSRNTGYGAGIGGGGNGSGGDITINGGIITAKGAYHGAGIGGGYYTTTKNALS
                     PSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGTNRGYRILVTGGTILP
                     YMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDLEKKTKVFMNKISLTY
                     LGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQGILYLWFSDQFKGSEVSVNLVVID
                     KITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDTPPYLIIKRYDGLPFEEDVEKSFL
                     DWIFKDGGIPIITPKGEKLTDKKFMTISSQRLNEETMEPDINLGSDDLTKQVDAGKYQ
                     LSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTETSLVVEESKAKTDVFKPTDTFTL
                     NVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGSESHNYSTASIEWRPL
                     DSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDVDGDGIPDINIDTDGD
                     GRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDKNIDTNGDGNPDTDY
                     NRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDNGGNSKPDSGNGGNSK
                     PDSGNGGNSKPDSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGN
                     SKPDPDNGGNSKPDIDTDGDGKPNINIDTDGDGKPDINIDTDGDGKPDINIDTDGDGK
                     PDINIDIDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNVQTGDQSNIMLDLALM
                     FISLFFLIKNLTNKYLRRK"
BASE COUNT         4852 a         2674 c         1567 g         6069 t
ORIGIN      
        1 aatagaggtt gtattgagaa ctaaaattta gactttataa atgaatggaa ttttcattgt
       61 tgaaatactg aaaatatatt tgatttcata taaaagaaag tgcctcatga actaagttca
      121 ttttgagaca ctcccttttt tctccaagct attatatctt tttttaaatt ctaatggaga
      181 gactccaatt ttttctttaa atactctgta aaaagttttt gtacttccaa aaccaacttc
      241 ttcaaccaaa tctacaatgg atatattgtt attaagtaat atccactttg ctttttctaa
      301 tctatattca tttaaaaaag ttataaatgt ttttcctgta ttttttttga aaaatttagt
      361 aaaataatat acactaaatc ctacttcata agacacatct tctaatttta taggttgttt
      421 ataatttttt tctacatatt tgaatatatt gttcaatctc tctaatatct ctttagagtt
      481 tatttcatat tctactgtat tttcttcaat ttctctagga atctgtctat ataatatagg
      541 aattaaatta tacattttac cttttattgc ataactatat ccttctattt tgtataggtc
      601 ttcattgtag atatctatca atatatcgaa aactgacttt cttatttttg ctgaccaatc
      661 tctactacat ttttttatac tactgaataa attaattaag ctttctttct cctcatttaa
      721 taaaattaaa tcattaaaaa aagatatatc aaattgaaat actaatcttt cacttcccgg
      781 tgatggcagt atataatgaa tatctcctcc ttttattatc actatctcgc ctttttttaa
      841 ttgcataggc ttgtcattta taaatagatt tactacacct tctgttataa gtaatatttc
      901 tatttcctta tgccagtgtg gtgttgttaa tacttctcct tcatttgata gagtacgaaa
      961 aggaaattca tgtattaatt taggcatttc taaatatatt gccatatata tcctctcctc
     1021 tctagtgtct aaaaattaat ctatactatt taaaagaata cacattttat tttaaaaacc
     1081 tcaaatttat aatttataca atacctacac ttcataaaat atatctaata tttctttttt
     1141 tgtatgtgca tttaaaagtc tttcacctat atttttacca cctaaaatgt aagctatttt
     1201 agatattaat atcatatctt cattacctcc agattgtggc atagcaaaca taattattac
     1261 ctttacaggt aagccatcta tacttggcca atctacacct tttttagatt tacctattac
     1321 tatggatgcc ttctttacat attttgtttt tccatgtggc atggccattt gataacctat
     1381 actagtagag cctatttttt ctctatgttt taatgccttt ataaacttac tttttaaagt
     1441 tatacttcct gtatcgtata atttatctac taattcctgt attatgcctt cttttgtagt
     1501 tgatttcaaa tctaaaataa tgctttcttc tgttaatatt tctctaattt ccatttgttt
     1561 tctccaatag attattctta aataatattt taatatcctc tttgctcttg caatctatta
     1621 tatagtttat atagttcttt tgcctatata aatttgatat ttccattaaa atattcttta
     1681 aaaaactttg attactaatt tctaaataaa tatcaatcgt tctagtaaca ttattgatat
     1741 tcattatccc tttatttaat ttactttgct tatgtatata tatatttaaa tcttttccta
     1801 atgaaataga ttcatattta ctccctaaat tcacacctat taaactttcc atctgagcta
     1861 catcaaaatt aaaatatata ttatcttcta aatacattcc taataagttt gtatctaagt
     1921 tcatactttt tttaggttta gaaagtaaag cattttttat attattaata tcactttcca
     1981 ttaatagtac tgatacaact atatgtaatt tatctttcaa atctaagttt acagtagata
     2041 ttacaaagtc tacatcatct aaatttcgct tactcaactg atgcatagga actatatcaa
     2101 ctatagtcca ttcaggaaat tctcttttta atctggctgc aaggagctga gatgttccat
     2161 atcctgtatg acaaactact attactcttt tactactcat atttctttct attgccgctt
     2221 gaaaatatgt ggcaagatac gaaacttcat ctatactaat attcttcaga ttatcatact
     2281 ctgtcataat atttatagtc atcatacata atcccagcac atctgaaaat cgctcttgta
     2341 tctctcctaa caacgggttt tttatttgga tatcatatct taatctgttt aacataggcc
     2401 ttacatgaga tgctagactt ttctgtagta agtcatcatt tttgagattt atatttaaaa
     2461 atcgtgacat attctctatc attatagatg aaactttttc actttcactt aatctatctg
     2521 attcatcatt gtaactacta ttattttcgc tactaaatcc agatgatacc aaaaaaatat
     2581 atatgtacat aatttcatct ttcgttattt ttactctgta tctattttct atcttatcta
     2641 taagtgtagt gacattttta tatactaact cgtccaaatt atctacagaa atttctcttt
     2701 cttcctgaga ttctatttga ttaccttctt cgattctctt taaacatata agtatatggg
     2761 taattaaatt tatatagtag ggctgattta tagtatatcc taaattgtat tctaattctg
     2821 taattatagt ttctacaaat ataatagaat caatatcaaa taaatttatc aatgcactaa
     2881 atgtagcaga atctaatctt gttaactcag atatatcatg ttgttcataa ttttcatcta
     2941 ataaatcatc catcattttg gcaatcgatt tccttatatc cacttcttta cctataatct
     3001 tagttccttc taaagtttta ttaagtttta aattgtattc ttttatccat gcctctatat
     3061 atttaaaatc atttactata gaagttttac ttacataata tttatctgct aactgattta
     3121 ttgaagttat attatttgaa tttagcaata aatactttaa aatctccatt cttctctgct
     3181 ctactgataa agatttattt tccttcgatt gtatatttaa actattaatt aagtctatgt
     3241 tcttctttgc taaatcttct attaatattc catagcctct ttttatatca atttttatat
     3301 taaaagtttc taaatatttc tcaatcatct tcaaatcttt ttgtaaagtt ttatcagata
     3361 catttaattt atctttaaag tattttatag gtctatattt attttcgttt agtatcaatt
     3421 taagaaattc aatttgcctc gacacaagca tacttagccc tccctaccta tgtttatata
     3481 tttctatgtt tatattaagt actattttaa catatttact aactatgtaa aattactaag
     3541 atacttctgt atcataaaag aatttatgat acaccttact aaattgatat aatagataat
     3601 ataatttcaa aataaaaatt aaggatattt agaaatagtt aactgattct aaatatcctt
     3661 attattattc tatatacttg ttgttattcc attaattatt tttactatta tttataattt
     3721 aagcaccaag taattcaact tcactatttt cactccttat agtaggcttt gttaccccta
     3781 taatcaatgc tgttattact gtaccaatca atatacaagc tatatatgca actggtgcac
     3841 tacatgcaaa tggtattaca aatattcccc catgtggtgc cattaaagtt atatctaaac
     3901 tcatacatat tgccccacct atacaagaac caattatatt tgctggaatc attcttaatg
     3961 ggtctgcagc tgcaaacggt attgcgcctt ctgttatgaa agatgccccc attatagcag
     4021 ttggtagaga tgactttctt tcttctttag taaacttatt tttgaatatt aacatagcta
     4081 atgatattgc taatggaggt accattcctc ctaccattaa tgctgctgat ggttcaaata
     4141 tattacttga aaacatagcc aatccaaaag ctgaagctgt tttattaata ggtcctccca
     4201 tatctgatgc catcatccct gctaatattg cacctaataa tactttatta gttccagaca
     4261 tactttgtaa ccaagattgt aaagcttcat ttaaccatga tgctggcgta ttaactacaa
     4321 agaacattat agctccactc gtaaatgcaa tcaaaagtgg acatatcaat acagcattta
     4381 ctccttttaa tacatctggt aaattttctg ttatcttcat tactcctaaa gctatatatc
     4441 ctgttgcaaa accagctacc ataccaccta taaaacctga acctccaata actgccataa
     4501 aaccagctat catagcagga gcaaatcctt gtcttcctgc tattgattta gctataaaac
     4561 cagcaagtat cggtataaat aacttaaatg ctgaatcgcc tccaagttgt ccaaagaatc
     4621 cagctatagg gttgtatgtt tcatgagttg ggtctgatgc aaatatacca aacataaaag
     4681 ctatggctat accaattcca cctatcacta caaatggaat catttcagat acaccactca
     4741 ttaagtgctt ataaacaccc tttttctctg acccttgaag catttctttt gaatcttcta
     4801 aagtctttcc acctggttta atttctttag ccattccatt ttcagcgttt ataattaaat
     4861 ttttagctaa tgaaacattc ttagcatcta ctactttctt ttgtagcact ttctttccta
     4921 taaatctact cagttgaact tctttatcac aagctattat tacgtattca gctcttttta
     4981 tatcttcttt agtcaactta ttttctacac ctatagctcc atgagtttct attttaactt
     5041 catatccaag ttcttgccct gcttcctcta atgctttttt tgccataaaa gtatgagcta
     5101 tacctgttgg acatcctgtt atcccaacta taagaccttt attttgatta ttactaaaat
     5161 ctcctgattc tgcagcttta ttattaaata tacttaatat ttcgtatgga gtttctgcac
     5221 ttagtagatg cttaagaaaa ttttcttcca ttagatatgt agctagttta gatataatct
     5281 taagatgttc atctgaacct gattctggaa ctgctatcat aaaaaatgac ctaagttttt
     5341 gaccatcatt atctgtaatc tctctatctg ttcttaaata catcattaca ggttgattta
     5401 ctacatttga cttagcatga ggtatagcta ttaaattacc catattagtt gatgattctt
     5461 tttcacgttt attaatatca gctataaact tttctttatt atttatataa tttccatggt
     5521 acaaatgatt tgccatataa ttaatcacat ctttttttgt ttgtaattgt ttatctagta
     5581 caatacaatc gcttcgtagt attttaccta tcataaattc tcccctcctt ttaatttgtt
     5641 acctcataat taatttttat ttttataaaa gcactttaac ttatctatag cattgaatag
     5701 attcatttta aactctataa cttctcttgc agcttttata catggtggat atatagtatc
     5761 tggctcatat aagcttgggt tttgttctaa tagttctctg cacttcttat aatatgccga
     5821 cttaacatca ctcgaaatat ttattttaca aactcctagt ttaactgctt gtgctatttc
     5881 ttcatctgga tttgatgaac caccatgaag aactaaaggt atatctacta cttctcttat
     5941 ttcttttaat aaatctagtt taagctctgg tttaaaacct tttggatata taccatgtgc
     6001 tgtaccaatt gcaatagcta gtgtgtctac tcctgttttt tcaacaaaat ccttagctaa
     6061 ttttgaatct gtatatatta tttcatcagt accaccttca cttgatgttc ctgttgttcc
     6121 tattgttcca agttctcctt ctacggacac atttaatcct tttgctattt ctactatttc
     6181 cctagatata gctacattct cttcatatgg taaatgtgag ccatcaatca ttactgatgt
     6241 aaatccacat cttattgccc ttaatatttg ttctttattt tccccatgat ctaagtgtat
     6301 taccataggt actttactct tatttgcttc ttctatgcaa ctagctacaa agctatctcc
     6361 tagaaaactt agctcatttg ggtgtattgc aagtattact ggtgcatttt tttcgttagc
     6421 actctgtact actgctttaa gtatttgccc acttcctata ttaaatgctg gtacagcaaa
     6481 ttgattttct tgtgccactt ttagcatttc cttcatgttt attaacatat tgtacctccc
     6541 gatattttaa attttaggtt gaatttgctt cttgttaatt ttgttgttgt ttatatttaa
     6601 ataatatcag ggtaatgaat gtcagaaaat aacaatcgtt ttccttagtg aaaaggaact
     6661 tttattatac actctaagtt ccttttcact aaataatttt tagaagtctg catcatagtt
     6721 aattatattt ctcatgtaca tcttctcttt ttaagtgttt tatgctttag ccttcctaga
     6781 ttttataata aacaattaag atacattaac tataaattta aagatactca tgcaatagtt
     6841 aatgctatat tagagtcaaa aaaattaaaa agatattagc tatttggcca ataccttttc
     6901 ttggattcca atagtatata cgatttatag aaatcttact cacgattatc taatgtttta
     6961 cttccataaa ctttacataa aacaccaaag tgacaccctc caaattcatc tctagctaat
     7021 aaagtgattt ggttttctaa gcaccaattt ataaattagc ctttgttatc ttatgtaaat
     7081 ctttttctat ctaatttaaa aattattcct aatgctatat agcatatacc aattactaat
     7141 acaccataag ccatagatac tcctatctta aattcttgca tattaattac aatagaaact
     7201 gaaatagatt tattacaaac accataaagc aaggctgaca ttgtatatta tccaattgta
     7261 tgaatgaata ctaatatagc tccttttaat taaatgatgc tatataaatc atatttatcc
     7321 caatttttaa tctaacctat ttatatgtat attccttttt aataaaaaaa gatattgact
     7381 aaatagtcaa tatcttttag ttgatttaaa tacccacaat tttaatctaa tttttaagtg
     7441 tttccttaac ataatcttat aaatattaaa atttatctac ctataaaatt tctccattaa
     7501 attttattac attttatctt aaaacaatta taatctgacc ttaatcatat attatattat
     7561 ataaatattt ttaacaaact taataattaa ataactatat tttattatcc atcttataaa
     7621 tgtttataaa ctttccacta ttcttcttca ataaattcaa acaaattttc tactgtagta
     7681 tcgaatactt tagcgatatc catagctaac ttcaaagaag gattatattt cccattttct
     7741 aagtgtacaa tagtctcacg tcttacccca accaatttag caagttcatt ttgcgtcata
     7801 aggagttttt ctctgtattc tttaattttt gttttcatca atattgccac ttagttacat
     7861 atcccccttt tatcaaagta tatatacaac actaatttag ataaagactg tataaataaa
     7921 actgcaatga ggattatacc tgtatctagg tttgatatat ttatattact tgtataaggt
     7981 gttgcaaaga aaatagatat tattactata gaatatatca ttatttttat tgcaatctta
     8041 ttaacctttg atagtataaa ttcagatgat tcatcaacta aatcattata ttttttatta
     8101 taaattgaat aacatccaaa aactattaac caactcaacc ctggaagaga agcagtgctt
     8161 ggacctttaa aaatatactt agtggctaat acagctataa aaaaataagt tattacaagc
     8221 aactccagga taagctttga tcttacacca gtttctgatt tagacatata aaatcactcc
     8281 ttaaaaatta tattctctat actctttaat tttcgttttt atcaatatta tcacttagtt
     8341 gcatattccc tttctatcaa aatgtatata caaaattaat ttagataaag attgtataaa
     8401 taaaatagct aagagaatta cacctatatc caaatttgat atatccaaac ctcttgtata
     8461 atttgctgaa aagaaaacag ctattagcaa aatagaatat aacattattt ttgatgcaat
     8521 atgatcaacc tttaataaca taagttttga cgcctcgtca actaaatcat tatatttttt
     8581 attatacatg aagtcacatg cccaaaaagc gaataaaatt ataaaagtaa tacaaagatt
     8641 tccctcttta aaaataaact tttttaataa tgagtctata aaaaaatagg atatcataag
     8701 taactctgaa acaagatttg ttttaacacc aagctctgat ttagacatat aaaatcaccc
     8761 cttaaaagtt atatatttat aacaccttat gttataaata tataactttt ccaaataaat
     8821 tgcaatagaa tattgaaaat attcaataat atcttcataa atatatttta taaaagattt
     8881 tataataaac attacaatac agtactaaat tagtttattt catctatcat ctcaatcata
     8941 tcatttacta actattattc taatgagcat ataacattga aacttataat tatacaacct
     9001 aaatgcctcc caatgtaccc tcaaaacatc atttcctgta actgaagtat aattggtata
     9061 ttttatcact gaaataaaaa aacactagtc aataaagact agtattttca agtattttat
     9121 cattcttttt ttaattattg gagacgaatc atcagaataa tgcccataat ttatctatat
     9181 gcaaattcta aatttaaaat aaagtttcag ataaatttac tattctatgc tatgatacaa
     9241 cagaaaaaac tgaaatctat gagtggtagc ttcatataat cctagttcct acagtaatca
     9301 aaactgaggt ttattctata tgtaactaat gataaatttt attataggag ttattagatc
     9361 tatttgtgtt actataagta gtaattatat atatgataat tttttaacac ttttctgttg
     9421 gctatataga aagtggttta gaatttaaat ttaaataaaa tttaaattca ccaaaaagaa
     9481 atctatatta gaaaccacta ctctattcca ccagattgta attttttgtt ctttatatca
     9541 caatgctata ccatattaaa ttgaaaaata agttttaaat ttatttgtta caattcatta
     9601 attcaaaaaa atattttgag tcatgagttt tgtaaagatt tagccatgaa tttaatgctt
     9661 ctgattcaaa aacatctaaa agtttcaata cttgaaacgt aaattccaac ggagcaattc
     9721 ctgaagccgt aactaaattt tcatcactaa ctacagattc cgttttataa tatttttctc
     9781 cagagtaatt tggtattacc attttaagaa actctaagtc attacttgta tgatttctaa
     9841 aatctaatag ccctttttta gctaggccta atgtagctcc acatatggct gcaactatag
     9901 aatcttgtct taaagcttct ttaacttttt ctaatattat ctcatgattc atagataacc
     9961 aagtatttcc ccctggcaaa attaataaat ccttactttc catattaaac tcatcaactg
    10021 taatttcagg taaaactttt aacccaccca ttgttataat aggattttta tcaattccta
    10081 cagtaattac ttttagtggc tttaatcctt ttttaaaata tcttcctgta tttaattcag
    10141 cagttaaata tgccacttcc caatctgaca tagtatcaaa tacatacata tatacttttc
    10201 tatttttcat cttttttact ccttatgtaa ttaatttata tatctaatta taaaataact
    10261 taactgacat attcagtcag ttaagctatt aaatttgata ataatcaatt aattctagta
    10321 aatcttcaat tattttcttc ctaaggctta gtggttcaat tacttgtatt gtctttctat
    10381 aaggtaaaag tatatttggt atgtacttgc atattacttc ctcttcaagc ataaatatta
    10441 ttttattagg tgttctttct ttcaagtagt gccctaaaaa ccaatgttgg cttaaactat
    10501 ctaaagaatc tatactccct tctataacta attgaattat attttgttca tcttttattt
    10561 caggtataat tccttttata aaaaattcac ttgctgaaaa ttctgtcggt ctgttaaatg
    10621 tatttgatgt ttctatgatt tcactaatcc tatccactct gaaactacga acctcatttc
    10681 tcatattaca aaatgcaact acgtaccaat tattattcca gtgaacaatt ccatatggat
    10741 ttataattct atattttgat tttactttac gacctgcatg atagttgatt tctacagata
    10801 gctcctttaa tatactattc tccaattttt taagtactga ttctatagac actttcccaa
    10861 ttggttttat tacttcaagg gctcttaagt ggtttgttaa aatatcttct tgtttttgat
    10921 ttgaatacat ttttaacttt aatatcgatt tatctaatgc ttcgtctaag aagtatcctt
    10981 cttttttagc aaatagtgct gcatgaagta gagatttttg ctcttctata tcaaaaaaaa
    11041 gaggtgcttt agtaaaatta tttaaaaggc tatacccacc attatgacct gtatctgaaa
    11101 ttataggtac tccactcgca gaaagtacat caatatatcg gtacactgtt cttatattta
    11161 tttctaattt ttcagctatc tgttttgctg taattttttt gtcagagttt aacatccaca
    11221 gtatagacat catattgtca attttagcca tgtagtttca cctctttcta tttgttatat
    11281 aaagttatta tataactttt tgattgattt tttacaaaaa aacattagta aactgctaat
    11341 aatttatact aagatttatt tcaaatattt atagatagtc tttttctata gttaagttat
    11401 ccattaaatt acatagtagt atatctattt tagttgatat attttcagct acctcccttg
    11461 aatattatta caataagtct aaatcttctt tcttccataa atatattatg aggaaagact
    11521 atcatcttta atggtttaat agtataaaag atattaattt aaaaaaatct atttttatat
    11581 cagatgggta ttgtttttta taaaactgaa aaaatcacct aatagaatct atgatttttt
    11641 aacataacct ccataaatta gttccaatta tcacttcaaa ttcaaatttt tatctgaaga
    11701 atatacaatc atatttccat actcaacata atattgagtg tttggcttgt atttacaaat
    11761 tattattcta acaaaaacaa ctatacatag attaaaatca tcttctaaac ataactacta
    11821 aaattgaatt aatgatatca attccattaa tctcttttaa taaaaaattc tcttttctat
    11881 atgaaaatta tttcaaaact tttaagttaa atacaaaaat gactataaat agatttttat
    11941 ttacacaaat ctatttatag tcactatagt ttatccaata aaataataaa ataaatatgt
    12001 acacaataaa tgttatttac gtcttaagta tttatttgtt agatttttaa ttaaaaaaaa
    12061 caagctaatg aacattaatg ctaaatctag cattatattg ctttgatctc ctgtttgtac
    12121 attttgacct acattactat ctaaactttc atctgtatct atatttatat ctggttttcc
    12181 attcccatct gtatctatat ttatatccgg ttttccatct ccatctatat ctatatttat
    12241 atctggtttt ccatctccat ctgtatctat atttatatct ggttttccat ctccatctgt
    12301 gtctatattt atatctggtt ttccatctcc atctgtgtct atatttatat tcggttttcc
    12361 atccccatct gtatctatat ctggtttgct atttcctcca ttatctgggt ctggtttact
    12421 atttccaccg ttatctggat ccggtttgct atttcctcca ttatctggat ctggtttacc
    12481 attgccaccg ttatctggat ctggtttgct atttcctccg ttatctggat ccggtttgct
    12541 atttcctcca ttacctgaat ccggtttgct atttcctcca ttacctgaat ctggtttgct
    12601 atttcctcca ttacctgaat ctggtttgct atttcctcca ttatctggat ctggcttatt
    12661 tagatttgga ttacctgtat cataacctcc tgagccagta tctacatttt tatcaggctt
    12721 ccaataatca tctactccat cattatctgt atctattgct ggacgattat aatctgtatc
    12781 agggtttcca tctccattgg tatctatatt tttatcaggt ttccaaatac catctccatt
    12841 tccaccttct gtactaggct tccattcacc tgtattatca gtatctatgt taatatctgg
    12901 cttccaatca ccactcgtat ctatattgat atctggcctt ccatcaccat cagtgtctat
    12961 atttatatcc ggtattccat ccccatctac atctacattt gtcatatcta actttaactt
    13021 cttttcatcc tcactacctt caatgtaatt tatatcatct ccaatatatt ttacagtaat
    13081 agtttgttca gtagtaaaaa tatgatgatt gctatctaaa ggtctccatt caatagatgc
    13141 agtactataa ttatgactct cactaccctt attatgagtt tttaattcta ctggatcttt
    13201 atattccttt ccattaatat agaattgtac gtaacctctt ggagaggcac aatctggtcc
    13261 ttctttctta tctggtgaaa tagtaacatt taatgtaaac gtatctgttg gtttaaaaac
    13321 atcagtttta gctttagact cttctacaac taatgaagtt tctgtatcag caggaataat
    13381 ttctgcctta taatatgctc tatgccccca ataagcatcc ttaaaaggat atatactcga
    13441 aaactgtgtt gatgtaatgc ttaattgata tttcccagca tcaacttgtt ttgtaagatc
    13501 atcacttcct aaatttatat ctggttccat cgtttcctca tttaaacgtt gagaactaat
    13561 agtcataaat tttttatctg tgagtttttc acctttagga gtaattattg gaataccacc
    13621 atctttaaat atccaatcta aaaatgattt ttctacgtct tcttcaaatg gtaatccatc
    13681 atatctttta ataatcaaat atggtggagt atcatcaata gtaaattctt catattgctt
    13741 taaaaattga gaacctgaac ctatatcttt aatataaaaa tcatctggtt ttagaacttc
    13801 tccagtaatt ttatctataa caactaaatt aacagatact tcagatccct taaattgatc
    13861 agagaaccat aagtaaagaa tcccttgagc atctgtataa ctaggtgctc cataaggata
    13921 aatttttcca tcaattagca ttttaaaatc atcaactagt gtagtactaa ctttatcttg
    13981 ccctaaatac gttagagata ttttattcat aaaaactttt gttttctttt ctaaatcacc
    14041 gtatgcaact gctccaccta aacttttgaa tgttgttgct tttaatgaac cacctgttac
    14101 tataacatct cccgttgtag cacttaggtc aaaaatccaa tctcccactc ctgtcatata
    14161 aggtaaaatc gttcctccag ttacaagtat acgatatcct ctatttgttc ctggattaac
    14221 acatccatcc ccaaatgcac taccatgtgc acctccatat gaataggaaa gtccaccatt
    14281 aattgtaata ttcccactat aaccagaacc tgtgctagga tctactactg agggacttaa
    14341 tgcattcttt gttgttgtat agtatcctcc acctattcca gctccatggt atgcaccttt
    14401 agcagttata atacctccat tgattgtgat atctccacca ctaccattac cgcctccacc
    14461 tattccagct ccatatcctg tattacgact cgattcttga cctatagcag ttatagcacc
    14521 accattaata ataatatttc ctccactttc agtatttcca ccaccaatac ctgcagaata
    14581 ataatttcca acaactttta aacttcctct attattacta tctagtagtg taaggcgatc
    14641 tccttttttt aacttgtctc cattttctag agttacatct ctaggaattt ttccaccctc
    14701 aggaactata aactctcctt caatatctct atttacaaca gaatcatcaa taattagagt
    14761 agtacctttt cctgctctca tacctggata atatacatca cctgactcta gcttgttttc
    14821 actaccatct tgtaatataa catgtgcata tgcaccacca tctttatctg gtgttaaagt
    14881 aaatgggcta ttagatgcag ttttaatatt tactccatca aatattaatt cagcttttac
    14941 atctttaggt ataacaatcc tatgtgttgt tggagtattt ggattgttat tttgaattgt
    15001 catttgtttt ccactaagaa tagttaaaac gccacttgca aatttaaaat cgacatttat
    15061 ttctccacca ctaattttaa agtctcctac agacgtaggc tcttctgcag tggcttcttc
    15121 ttctacaact actgcattag catctccttc tgcatctcct gc
//
LOCUS       DAYATN010000096        15132 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.096, whole genome shotgun sequence.
ACCESSION   DAYATN010000096 DAYATN010000000
VERSION     DAYATN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.096"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            198..875
                     /locus_tag="ACGI8A_002649"
     CDS             198..875
                     /locus_tag="ACGI8A_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0180640.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            947..2962
                     /locus_tag="ACGI8A_002650"
     CDS             947..2962
                     /locus_tag="ACGI8A_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0180641.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLYFL"
     gene            3012..3692
                     /locus_tag="ACGI8A_002651"
     CDS             3012..3692
                     /locus_tag="ACGI8A_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0180642.1"
                     /translation="MQTKILIIDGDKDNCKKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            4183..4656
                     /locus_tag="ACGI8A_002652"
     CDS             4183..4656
                     /locus_tag="ACGI8A_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180643.1"
                     /translation="MFVICISIYLAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQ
                     ILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGG
                     IGFKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIHTYCKHQVS"
     gene            5037..5954
                     /gene="pstC"
                     /locus_tag="ACGI8A_002653"
     CDS             5037..5954
                     /gene="pstC"
                     /locus_tag="ACGI8A_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFH0180644.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            5956..6780
                     /gene="pstA"
                     /locus_tag="ACGI8A_002654"
     CDS             5956..6780
                     /gene="pstA"
                     /locus_tag="ACGI8A_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFH0180645.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            6799..7563
                     /gene="pstB"
                     /locus_tag="ACGI8A_002655"
     CDS             6799..7563
                     /gene="pstB"
                     /locus_tag="ACGI8A_002655"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFH0180646.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            7591..8244
                     /gene="phoU"
                     /locus_tag="ACGI8A_002656"
     CDS             7591..8244
                     /gene="phoU"
                     /locus_tag="ACGI8A_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFH0180647.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            8402..9271
                     /locus_tag="ACGI8A_002657"
     CDS             8402..9271
                     /locus_tag="ACGI8A_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFH0180648.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            9358..10794
                     /locus_tag="ACGI8A_002658"
     CDS             9358..10794
                     /locus_tag="ACGI8A_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HFH0180649.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     NLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            10842..11669
                     /locus_tag="ACGI8A_002659"
     CDS             10842..11669
                     /locus_tag="ACGI8A_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0180650.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    11965..12227
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12303..14969
                     /locus_tag="ACGI8A_002660"
     CDS             12303..14969
                     /locus_tag="ACGI8A_002660"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFH0180651.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         5992 a         1577 c         2742 g         4821 t
ORIGIN      
        1 aattagtatt taataatgaa tcttaatgtt taatacaata ttcaagaatt attcaaaaat
       61 acttaagtat tatagtctag tttgtaaaga tattataaaa taataatacc agttatatat
      121 gaaaataaaa cattataatt tgtaataact ataaaacttg gaattataat taaaatacct
      181 ttgaatggag tggtgtaatg tataatttac tggttgtaga tgatgatgtt gatattttaa
      241 acataaatca aatttatttt tctaatatag gttttaatgt atatacatca gagactgcta
      301 aagatgctat ggatatagta gagagtatag ttttagactg tataattttg gatatttcat
      361 tgccagatat agatggatat aaaatgtgca atgatataaa agaaaaggtt aacataccta
      421 taatattttt atctaattat gagcatgaag aagaaaaagt tcggggattt ttagctgggg
      481 gggatgatta tataacaaaa ccttatagtt taaaagaact tgaacttaga atatatgctc
      541 gtatgaggca gtacaaaaat attacatcag ttcctaatgt gttaaagttt tcagcgttag
      601 taataaatgt aaattcaaga aaagtaactt ttgatggaga aagtatagat ttaactacta
      661 tggaatttga aatattattg tttctagctg aaaacaaaga acaagtattt tcgaagatag
      721 agatatataa ccaagtttgg aaaatgcctg atattggaga tcaacataca gtgcaggttc
      781 atattgcaca gatgagaaaa aaaattaatt ctttaagcag ggagcatcaa tacattcaaa
      841 ccgtatgggg aaaaggatat aagtttgtac cataagtgag atactgtaat taataaatag
      901 ttcttgtttt atttaatact actatgaaag agatgtggtt gatacaatga aaaagtataa
      961 tatactaaaa ggaactctca ttgtattgat agtctctctg ttggcaataa caggattaaa
     1021 tcttctgtat aaatatgata ataaatatac atataagtct attagagcaa gtaatggaat
     1081 tttaactata gataatgata ctttcactaa aggtaaactt gtctttttga tagatgactg
     1141 ggagttttat aatcataaaa tatttaaacc gaatgatttt acagagcaag atataaaacc
     1201 agaatatgtt tttataggtc aatatccaga tttttctatg agaaagggga ctcagtcacc
     1261 atatgggaat gctacttatc gtatgagaat aaaaaatgaa ggcaaggata agctactaag
     1321 tttagagctt cctgagattt tttccgcaag tgaagtatgg ataaatggag aaatggtttc
     1381 taaattagga gatgtaggaa cagaaaaata tagacccaaa attaaaaatt cagtagtaag
     1441 ttttattgca aagaaaaata cagaaattat aatcaatgta tcgaattttt cacattatta
     1501 tagtggtttg tattatccgc ctgcattagg agaaacaaag gacataagta atatgatgtt
     1561 ttatcgatta cttttttata gtataatttg cttcacaact tttgcaatag ctatattttc
     1621 attgtctgta tggtttcttt caaacaaaag taaaatttac ttatattttg gatgtatgtg
     1681 tgttttcttt gccatccatg tatcctatcc ctttgtacat ttgttaggac tgcctttggt
     1741 aagctttaca tatgcggttg aagatttgtc atattttatg gtagttttat gtattatttg
     1801 tataaatgga agtatatcca aaatagaaga aaataaagtg tacagatttt taattttacc
     1861 attaggaatt acaatgagta tcatatctat agtgatacca attattcttc ttccatatga
     1921 tatgtacttt gtcaacatat atgggaagtt aatagacata tataaatata ttgtatttat
     1981 atatatatta atgtcttcgc tggtttcaat atataaaaag acatgctctg agcatataag
     2041 ttcttatatt ttagtttcag taaacactgt ttttggaatt agcatattat ttgatgcagt
     2101 tacgagtaat agatttgagc ctatatttac agggtggcaa acagaatatt gtgggtttgt
     2161 aattgttatt ttattttctg ttatgatgat aaacaggaat agaataataa taaaagagaa
     2221 tgaaaaactt acaaggaatc ttgaacatga agttgaagtg agaactaatg aacttacaac
     2281 attgttaaat gaacgtaaaa gattcttggc agatgtagca catgatttaa aggcacctgt
     2341 ttcggctata caagcattta tagatttgat aaaggttggt aatattcatg tagatgaaga
     2401 gacaagaacc tatctttatg ctataaatca aaagtcaaat gaagtacaaa atagagtacg
     2461 tagtttgcaa gaatttacat cacaagataa aagcattggt attagtgaaa aaatatgtct
     2521 gaataatttt attaaagaga tatatgaaaa taatttgcca gatacacaag catgtggtgt
     2581 aaactttgaa acttacgtat tggagaaaaa aatatatgca aagataaata aagagatgct
     2641 tacaagagca tttgagaatt taatttataa tgctttgacc tttactccat ttgatggaaa
     2701 gataatttta tcaatggata aagaagatgg atttgcaatt attaaaattt ctgataatgg
     2761 aaaaggtata cctccagaaa atataccaaa ggtatttgac agattcttta cagaaagaga
     2821 caataatgag ccaaaagggc aagggttagg gctatatatt gtcaaatcca ttataagaga
     2881 gcatggtgga gaaatctttg tagattcaga actaggaaaa ggaactgttt ttacaataaa
     2941 attaagtcta tattttttat aataatagct tatttaatat aaaagaataa tgatatttgg
     3001 gagtgattat aatgcaaact aagatattga taatagatgg agacaaggat aattgcaaga
     3061 agctaaaagg atttctggag gaaaaaggaa taagtataga tttagcatat aattgtgaag
     3121 aagctattgg aaaaatattt agcaataaat atgatttaat atttttagaa ataattctaa
     3181 gtgatggtga tggatggact ctttgtaaaa aaattagaaa tgtaactact tgtccaattg
     3241 tttatatgac ttatataaat gaagaccaaa gcattttaaa tgctttaaat tctggtggag
     3301 acgattactt aataaagcct ctaaatttag aaatattata cgcaaaagta aaagctattt
     3361 taagaagaat gaactcatat gtaaataata atgaaaataa tacaaaatta gaggaatata
     3421 atagaactag tggagtaata aagttagaag ataaaataat aaaattgact ccaacagaaa
     3481 ataagttgtt aaactttttt atagagaatc ctgaaaagac tttgactact aaagagatat
     3541 atgaacatgt atggatgaat gaatacttag aggataacta tagtgtggta gttgcagtga
     3601 atggattaag aaagaaaata gagaaagatt ataaaaatcc tcagaaaatt ataacaataa
     3661 gaggagcagg atattatttt aataaagaat aataaatata ttaatatcat taataattgt
     3721 tataaatgtg aaatactaac aatatctaaa ctaaattatt ataaaatagc tgactgtaat
     3781 ttaattgata aagcctattc ttctagactt tgccagttaa attacagtca gttttattat
     3841 ataaaaaaat gcgtaatgga actattattt tatagattat atttgaaaag atagataaat
     3901 tgtatatcta aatctatata ttattttttt acctttaata tatattaaat aataaaatag
     3961 ttaaataata agattaagaa tttcttaata aatccagaag atgctttcga ttatataata
     4021 ttttatattt ttatattatt aattatatat gttgcaattg ctgcacctac tttttctggt
     4081 gagtatcaga cacaatcaga tagcatcctt cgttgtacga aacatggaca tatttgtcta
     4141 gcgtttacta aaatattagc tctattttct atatttataa taatgtttgt tatatgtatt
     4201 tctatctatt tggcaatttc tgatttagct tttggtactg aatgtttgaa aacctcattt
     4261 caaatgttgt tttctgtcat tagtctagta aatataaatt tactacaagc acagattctt
     4321 atagctattg gtggtctaat ttcaatactt gcaatggtta gtttaacatt atttttatct
     4381 gcaaaatgta aggattctct tacttctatg ctgattgctt tttctatttg cttaattcct
     4441 atgggaatag gtgttagttg gattggatac ctattaccgc cagggggaat tggttttaag
     4501 attagtttat tatatcaaat gacatcattt aatttcttac acttaggagg tatgagtttt
     4561 tggacaccac acattatact gtttttctca gtgatagaaa tacctatttt tatatttttg
     4621 gctattcata cctattgcaa acatcaagta tcgtaacaat ttgatggaaa taatagtgat
     4681 ataaactaga ggatacaata attatatatg tagtataaaa gttaaattta gttaacaaat
     4741 cttaatagca gatataggta ataaattata cataaactgt attattatct atatctgcta
     4801 ttttaatata gagaaacaac agtatattgt aaagtaaatg tcaaatatag ttttaaaagt
     4861 aaaattaatg ttaaaaagta aatataattt tacacttgct taacttaatc ctaatataga
     4921 tttaacttaa aaaaagtaga ataaaagtac aggaaatttg atgaagacga attataaaaa
     4981 attaaatttt gaatacagaa cgaaaatcga taagtatatt ttaatgggag ggtattatga
     5041 tagtaaaatc tagattagta ggtttagaaa aaaatgacaa taatggcaat aagacaaaat
     5101 acgtaattga aaaagtagca aaaaatattt ttctacttag tgctttagta gcagttgcaa
     5161 gtttactttt gataatagga tttgtatttt ataaaggatt aagaccattt atatttgaag
     5221 gatattcatt tatagacttt ttaactggtg cagattgggt tccttctgcc aataaatttg
     5281 gaatatcatc tatgatagta gcatcaatat ttgcaacact tggagcactt gttataggag
     5341 tacctgttgg tatattaacc tctgtattta tagcagaagt agcacctaaa agattagcaa
     5401 aaataatgtc accagcagtt gaacttttag caggtatacc ttcagtatta tatggtgtat
     5461 ttggtcttgc agtgatagtt ccaactatac aaagtatatt taatcttcca aaaggtcaaa
     5521 gtttgttagc aatagtaata gtattagcag taatgatgtt accaacaata atatctgtat
     5581 cagaaacagc aataagagca gtaccaaaag catacaaaga aggttcatta gcacttggtg
     5641 catcaaaaat agaaacaata ttcaaagttg tattacctgc tgcaaaatct ggagtattag
     5701 ccgcagtagt gttaggagta ggaagagctt taggtgaaac aatggcagta atacttgttg
     5761 caggcaactc accagttatg ccaactgcaa taacagatag tgtaagacca cttacaacta
     5821 atatagcact tgaaatggga tacgcatttg gtacacatca agaaatgcta tttgcaactg
     5881 gagtagtatt gtttacattt atattaattt taaacttagt tttaaataaa ctttcaaata
     5941 aggcggtaaa ttaagatgag aaagttcaaa gagaatctat tgaagtcatt agtatatcta
     6001 tcagcactat ttacaatagt atcattagta gttatagtgg gatttatatt tataaaggga
     6061 attggcaaca tgaattttaa tttcttattt agcaattatt cagcatcagg tgatggtggt
     6121 atacttccaa tgataattac aacattatat acagtagtag tttctatagt tgtagcaact
     6181 cctataggaa tattggctgc tatatattta caagaatatg ctaaaaaagg aaaagcagtt
     6241 acagctataa gatttgcaac tgaaagttta tcagggatac catctatagt atatggatta
     6301 tttggaggaa ttttctttgt agttacatta aaaatgggat attccatagt tgcaggttca
     6361 cttacagttg caataataat attaccagta ataattcgta caacagaaga agctttaaaa
     6421 acagttcctc aagcttatag agaatcatca ttggcattag gagctacaaa atttcaaact
     6481 ttatataagg tagtacttcc aagtgcaata ccaggaattt tatctggagt aatcttatca
     6541 gttggtcgta taattggaga atcagctgca atattactta cagcaggtac tgtagccaag
     6601 atgccaggtg gaatatttga tagtgcaaga acattaactg tacactcata cttactgact
     6661 aaagagagtg gagatatagc aacagcagcc agtataggaa ttgttttaat agtaattgta
     6721 ttagcactaa acatgctagc aagatttgtt gctaagaaat taaataaagc taattattaa
     6781 atttaagagg tgtgaataat ggaattaatc gataagataa aaatgagtgt aaaagatttg
     6841 gatttatttt atggtgataa gcaagcatta aagaaaataa atatggatat aaaagagaat
     6901 aaggttactg ctttaatagg tccatctgga tgcggaaaat caacttttat aagaacgtta
     6961 aatagaatga atgacttaat cgaagatgta acaattaaag gaaacatttc tgttgatgga
     7021 gaggacatat atacctcaga tgatgttata aatcttcgta ctaaagtagg aatggtattt
     7081 caaaagccaa atccatttcc tatgagtata tatgataatg tggcatatgg tcctcgtaca
     7141 catggactaa gagataagaa gcaattagat aaaattgtag aagaaagctt aaaaggtgca
     7201 gctatttggg atgaggttaa agacaggctt aaatcatctg cactaggact ttctggtggt
     7261 caacaacaac gtatatgtat agcaagagca attgctatga gaccagaagt tattcttatg
     7321 gatgaaccta catctgcttt agacccaatt tcaacattaa aagtagagga acttatagag
     7381 gatttaaaaa aagactacac aatagtcata gttacacata atatgcaaca agctgctcgt
     7441 atatcagatg aaacagcttt tttcttaaat ggagaggtta tagaatttag cgatacaaaa
     7501 actatgttta ccactcctgt agataaaaga acagaagact atataacagg tagatttggt
     7561 taaaatatat aactataaag gagttagaca atggtcaata atagcttaga tataagtatt
     7621 gatactttaa aagaacaaac agtgcttatg atggaaaaat gtgaagaagc aatagaaaag
     7681 tctgtgactt gtatgataga gaaggattta gagggtgcaa gaaaagtagt agaagatgat
     7741 gaagaaataa ataaattaag agaagaaata agagataaga gtatagagct aatagtatta
     7801 aagcaaccaa tggcaaggga cttaagattt atttattcat taggtaatat atctatagag
     7861 ctggaaagaa taggagatta tgcagcaaat atagctagag aaatgttgaa gataggtgga
     7921 gaggcttata taaaagagct aatagatatt cctaagatgg caaaagaatg taagtctatg
     7981 atgtctcaga ctagagaagc tttaatctta tttgatgcaa gtatagctta tgatgttgga
     8041 actaaagatg atattgtaga tggattgtat gaacaagtac aaattgattg tttaaaaata
     8101 atgcatgaaa actcaaatac catagaccaa ggggtaaaac ttctacttat aggaagatac
     8161 cttgaaagaa ttggagacca tataacaaat gtatgtgaga agataatttt tgcaattgag
     8221 ggtaaaatgg ttgagatagg ataaaaaact gctttaaagc agttttttta ttgtctaaat
     8281 taagaaagaa ttgataaaaa aatatgatta atgggtaaat ttaaaaaatt gtgatatact
     8341 aggaacatat taaatatgag gatgcgtatt ttatataagg tgctttttag gaggtggaac
     8401 tttgaaactt caaaagatgg gtatgaggac tataaaaaca ggaattagtg tcactttatg
     8461 tatgattctt ggtgactatt tagtagagaa tatgttttat tctctggtag catgtataat
     8521 ttctgttcaa gatacagtaa aaggttcttt aaagacggga atcaatagag tggaaggtac
     8581 tattttaggt ggtataatag gattcatatt tgccatcatt aaagctggag acccattttt
     8641 atgtggtcta ggagtaatgt gctcaatata tttatgcaat ctgtttaaaa taaagtcagt
     8701 agtagttgcc tgtgtaacct ttctggctat acatctaggt gtaggaaata gtgaccctgt
     8761 gtattattct ttacataggg tgttagatac atctgtgggt gttttgtttg gagtacttgt
     8821 taattattat gttgctagac caaattatct aaatagtgtt atagatgagt ttaaaaagat
     8881 agaagaactt tcaatgttgc ttattgaaga taaaattctt agaaaagaaa atttaaatgt
     8941 agagaaattt gaagaagaag tagaaaaatt agaagaaata tattcaaaac ttattgatga
     9001 gttggattat agtttgagtg aaactaatat agaaaaaatt gagaatgcct taaggatatg
     9061 tagagaaata cattttcata tgcagtctat agagttactt gaaaataaac tttatttgaa
     9121 agagcataca tataaatacc ttaagaaaac atataatgtg gataagctag actggaatat
     9181 agatgaagaa aaaagccctg tattcaacta tcatctaatg aaaataatga ttgaaataga
     9241 gttgttgaga aaactaaata aatcatatta ataatagtag ttaaataata aataaaaaat
     9301 agtatctttg taaatattaa acatatataa gtttaattta tggaggtact attttttatg
     9361 aatactagaa atgacaattc taaaatacta gaaatatata aaatgaagct ttttggcgaa
     9421 cttgtaaaag cagtgtggaa tgatgacata gaggatattg aaaacttacc taataaaatt
     9481 ttaaatgaaa atttaaagga taatttaaat aattatttta gtggaaaatt tgataaagat
     9541 actatggtaa atttaattag ggtaactatg ggattggatg tgacagaaga tacagataaa
     9601 aatttaaaag agtcactaca tgaagctgta aatttagatg aagtcaaaag accaataata
     9661 tctgtaatat cagatgcttg caaatattgt gatgaaaata ataaggaaga tgagtcatgt
     9721 aagataagaa gtaagcatat taattgtaat gaggaagatg tatgtagtgc ttgtggtgat
     9781 tgtgtatcaa gatgtaagtt gggtgcaatt tcagataaaa tagaatttat accaatgata
     9841 aaattgttaa aagatataaa gagccctgta tatgcaatag ttgcaccagc ttttgtagga
     9901 cagtttggga aagatgtaag ccctggtaaa ttaagaactg cattaaaaaa gataggattt
     9961 gaagatatga tagaagttgc tttggcagca gatatgctta ctgcaaaaga agcatatgat
    10021 tattatgagc atatgaaaaa ggacgaagaa ggatgcttta taactagttg ttgctgtcct
    10081 atttgggtta gtttgataca aaatagtttt cctcaaatat tggagaatgt atcaccatcc
    10141 gtttctccta tgattgcttg tgggagagct gtaaaaattt taaatccaga tgctaaagtt
    10201 gtattcatag gtccttgtat ggcaaaaaag aaagaggcaa tgttagaaga tataaaagat
    10261 gcagttgatt ttgtgcttac atttaaagag ttgcaagaaa tatttgatgc attagagtta
    10321 aatccagcaa taatggatga gagtaataga gtagaggcgt ccctttcagg tagagtttat
    10381 gctagaacag gaggtgtaag taaagcagtt aagttatcag ttagaagaat tgacaaaaat
    10441 ataaaatttg aagaaaaggc atttgaagga acaaaagagt gcatggacgg tctaaagaaa
    10501 gttctaaata aagaaataga agctacattt atagaaggaa tggggtgtgt aggaggctgt
    10561 gtaggaggtc cgaagcgtat tttaagtgtg gaagaaggta ctaagaatgt tgatgaatat
    10621 agtgaaagta cgaatatgga aactccattt gataatttaa atgtattaca atttttaact
    10681 tctttgggaa tcaaaagagt agaatcttta ggagaaaaag aagaagaaaa ggttaaaaaa
    10741 atatttagta gagatataaa aagtaataag aaagagaata aatataaaaa ataacttttt
    10801 aagtcttaaa cttatgcttt tataaaaggg agatatgggg gatgaaaatt aaagataata
    10861 aggctgtttt tcatgtattt ataaggatat ttcttatagc tgttttaatt ttaactggaa
    10921 cattattgga ggaaaaaatt aataatgttc cagttgaaaa tataagttca aaaggtgaaa
    10981 atacactgga actgctttct caaagtaata ctttaggaga aatcatatac aaagcatctc
    11041 aagaagaaac aaaaggtcca ggggctactt ctgaaaaagt agcatacata acaatagatg
    11101 atggtccatc aaaatataca aatgcactac ttgatatttt aaaagaaaat gatgtcaaag
    11161 cgacattttt catgcttaat cagaatatga aaaaccacaa ggaagaagta aggagagtct
    11221 tggaagaaga aaactcaata ggttttcata gtgtaagtca tgatatacat aagttatata
    11281 aaagcccaga agtaacagtt ggggaattta atacatgcaa taatactcta caagaaataa
    11341 ctggtcagag ttcaaaatta ataagattac catacggaag taaaccatat atgccagaag
    11401 gttcatataa taaacttatg gcaaatgatt atttaatttg ggactggaat ttggatacac
    11461 aggattggaa gtcttctaca agtcagatag taagtaatat attatattat ggaagaaaaa
    11521 gagatgaatt agtagtatta attcatgaga aagagcaaag tttaaatgct ttaaataata
    11581 ttataagaat cttaaaagag agaggatata caatattgcc aattacagaa aatataacac
    11641 ctaaaaattt ctggcaagaa aacttataat aaatttaaat atagtaaaag ataaagtaaa
    11701 ttatttataa aataaaacat ataatataaa aaatacgaat aagaggtata ctgtaaggat
    11761 aatataaaat taatgagaaa gcattaaaat taaaataaaa taatcttgca attattgaca
    11821 ttataagaca gaataactgt aaaaacatat tttaaaatat aacaactata taaaagagaa
    11881 tagacataat taagaaaatt cagaaaaaat aaaataaaaa taattgacaa agagaataat
    11941 atttgtatat aataatatgt aatattaaag actgagaaga agaggagtaa gtaaatacta
    12001 ctaaaagaga ggaaaattca ctggctgtaa gattttctaa gtataagatt tactgaatgt
    12061 agctttggag tttctaaact gaactataag taagtttaga cggttagaac cgttaaattc
    12121 tttgagagtc aaagttgata aaataacatt aaccaataat ttaatgatat gttaatgtat
    12181 tttaagcttt ggaattaagg tggtaacgcg agcttttcgt cctttttaaa gaggatgaag
    12241 agctcttttt tatttctaaa gatatttaaa aaatttattt ataaaataca ggaggcagga
    12301 caatggaaaa tacgaattta tcaaaaactt ataacccaaa agattttgag gctaggctat
    12361 ataaaaaatg gatggatgaa gggtatttta aatctaaacc aaatccagat aaaaaaccat
    12421 ttacaataat gatgccacca ccaaatataa cagggcaatt gcatatggga cacgctcttg
    12481 accatacact tcaagatata ttaatcagat ggaaacgtat ggatggatat gaagcttttt
    12541 ggcttccagg aactgaccat gcttctatag caacagaagt taaagttgtt gaaagaataa
    12601 aaaaacaaga aggcaagaca aagtatgaaa taggtagaga agaattttta aagagagcct
    12661 gggaatggaa agatgaattt ggaggaaaaa tatctaatca gcttaaacaa ctaggtgatt
    12721 cttgtgattg ggataaagaa agatttacta tggatgaagg gtgtaatgaa gcagttatag
    12781 aattttttgt aagcctatat gaaaaaggtc acatatacag aggaaataga ataataaact
    12841 ggtgcccaga ttgcaaaact actttatcag atgcagaagt tgaacatgaa gaacacgacg
    12901 gaaactttta tcatataaag tacccactaa aagatagtga agacttttta gaaatagcta
    12961 caactagacc tgagacaatg ataggagata ctggtatagc agtaaatcca gaagatgata
    13021 gatataaaca tttaatagga aaaacagcaa tacttccatt agtaggaaga gaacttccaa
    13081 tagttgcaga tagttatgtt gatttagagt ttggaactgg tgcagttaag atgactccag
    13141 cacatgaccc taatgatttt gaagtaggat taagacataa tcttgagcag ttaaatacta
    13201 tgaatgaaga tggaactatg aatgaagttt gtggaaaata tgaaggtatg gatagatttg
    13261 agtgtagaaa agctatcgta gcagacttaa aagaacaagg ttacctaatt aagataaaag
    13321 agcataatca taatgtagga acttgttata gatgccatac agtagttgag cctagattat
    13381 cagagcaatg gtttgttaag atggaagaac ttgcaaaacc agctatagat attcttaaaa
    13441 aaggagaatt agaatttgtt ccagataaat ttgataaaac ttatttacaa tggctagaaa
    13501 atataagaga ttggtgtata tcaagacaat tatggtgggg tcatcaaata ccagcttatt
    13561 attgtcaaga gtgtggagaa atagtagttg ctagaaaaat gccagataag tgtcctaaat
    13621 gtggaagcac tcattttaaa caagatgaag atgccttaga tacttggttc agttctgcat
    13681 tatggccttt ctctactcta ggatggccaa ataagactga agctttagat tattattatc
    13741 caacaagtgt acttgttaca ggatacgata taatattctt ctgggtagtt agaatggcat
    13801 ttgcaggtat gttctgtatg aatgaaaagc catttgacca tgttttggtt catggattag
    13861 taagagattc tcaaggtaga aaaatgagta aatctttagg aaatggtatc gacccattgg
    13921 agataatcga gcaatatgga gctgatgccc taagatttac attaacaact ggaaattccc
    13981 caggaaatga tatgagattc tatatggaaa gagtagaatt tgctagaaac tttgcaaata
    14041 agttgtggaa tgcatcaaga tttgtattta tgaatattga tgaagatata attaaaaaca
    14101 tgactagaga aagtgttaaa gaggacttaa ctctagctga taaatggata atatcaagag
    14161 caaataatat tgtaaaagaa gcaactaata acatggataa gtttgattta ggaatagcac
    14221 ttcaaaagat atacgatttt acttggtcag aatactgtga ctggtatatt gaaatggtta
    14281 aacctagatt atatggagaa gatgcaaatg ctaaatctgc agcattatat acattaactt
    14341 atgttcttga aaaaatatta aaattattac atccatacat gccatttata acagaggaaa
    14401 tatatactca tcttcctact gttgaaggat gtataatagt atctgaatgg ccaaaatata
    14461 atgaagaaga taacatggct gaagaagaag atatgatgaa cttattgatg gaaggtataa
    14521 gaagtataag aaatgtgaga gcagaaatga atgtaccacc ttctaagaaa gctaaattga
    14581 taataatccc aagtgaagaa aaaatagaag ctattgagct tggtaaagac tattttataa
    14641 ctttagcatc tgcttcaaat gtagagatag caaaagataa atctaatgtt ccagaagatg
    14701 cagttggagt agttattgat ggtgttgaaa tatttatacc tcttaatgaa ttagttgatt
    14761 ttgaaaaaga aattgagaga ttatctaaag aaaagaaaaa gttagaaggc gaaataaaga
    14821 gagttaatgg aaagcttgca aatcaaggtt tcttagcaaa agctcctgaa agtttaattg
    14881 aagaagaaaa agcaaaaaaa gaaaaatttg aagagatgat taaatctgtt gaggaaagat
    14941 taacaaattt agaatctaaa ataaaataaa tataagaatt agtaaaaaca tacaaaaagt
    15001 atatattcta tactttttgt atgtttttct ttatatatat acaaatatga tgttataatt
    15061 ctttacagat gtgttatatt tttattttat gttgtgaata tatgaatttt atgtcttaaa
    15121 atttataagc aa
//
LOCUS       DAYATN010000097        14818 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.097, whole genome shotgun sequence.
ACCESSION   DAYATN010000097 DAYATN010000000
VERSION     DAYATN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.097"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            162..1076
                     /gene="murB"
                     /locus_tag="ACGI8A_002661"
     CDS             162..1076
                     /gene="murB"
                     /locus_tag="ACGI8A_002661"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFH0180652.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            1080..1793
                     /locus_tag="ACGI8A_002662"
     CDS             1080..1793
                     /locus_tag="ACGI8A_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFH0180653.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            1824..2681
                     /gene="rapZ"
                     /locus_tag="ACGI8A_002663"
     CDS             1824..2681
                     /gene="rapZ"
                     /locus_tag="ACGI8A_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFH0180654.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            2693..3799
                     /gene="yvcK"
                     /locus_tag="ACGI8A_002664"
     CDS             2693..3799
                     /gene="yvcK"
                     /locus_tag="ACGI8A_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFH0180655.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            3868..4293
                     /locus_tag="ACGI8A_002665"
     CDS             3868..4293
                     /locus_tag="ACGI8A_002665"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFH0180656.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            4310..5257
                     /gene="whiA"
                     /locus_tag="ACGI8A_002666"
     CDS             4310..5257
                     /gene="whiA"
                     /locus_tag="ACGI8A_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFH0180657.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            5414..8983
                     /locus_tag="ACGI8A_002667"
     CDS             5414..8983
                     /locus_tag="ACGI8A_002667"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFH0180658.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            9192..10151
                     /gene="pfkA"
                     /locus_tag="ACGI8A_002668"
     CDS             9192..10151
                     /gene="pfkA"
                     /locus_tag="ACGI8A_002668"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFH0180659.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            10203..11963
                     /gene="pyk"
                     /locus_tag="ACGI8A_002669"
     CDS             10203..11963
                     /gene="pyk"
                     /locus_tag="ACGI8A_002669"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFH0180660.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            12138..13496
                     /gene="rlmD"
                     /locus_tag="ACGI8A_002670"
     CDS             12138..13496
                     /gene="rlmD"
                     /locus_tag="ACGI8A_002670"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFH0180661.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(13969..14607)
                     /locus_tag="ACGI8A_002671"
     CDS             complement(13969..14607)
                     /locus_tag="ACGI8A_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180662.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
BASE COUNT         5894 a         1607 c         2803 g         4514 t
ORIGIN      
        1 attatatatt tattgaatat agtttgtaat taagaagctt aaaatataaa atccatatga
       61 taagcaaaat catatattat tgaatatagt taatactata tattaatgta atttaaataa
      121 taatgacagc taaagttcag tataatttgg gaggaaatac aatgaataat caagatatat
      181 atataaattt attaaatata ttaagtaaag aggatataaa agtcgatgaa cctatgaaaa
      241 aacatatttc atttagggta ggaggaccag cagatatatt agttagacct agaactgaag
      301 aacaattaaa aaatgtactt aagttggtaa aaaaagagtc tattccgtat ttaataattg
      361 gaaatggttc aaacatttta attaaagatg gtggaataag aggagtggta atagaattag
      421 ctgataattt caattcttat gagataaatg atactaggat gactgctcag tcaggagctt
      481 tattatcagt tttagggaaa gctctccaaa agcaagaatt aaaagggttt gagtttgcat
      541 caggaatacc tggcacattg ggaggagcat tagccatgaa tgctggagca tatggtggtg
      601 aaatgaagga tatagtgaag tcagtaagac tgatggatat ggaaggcaat atatttgaat
      661 tatcaaatga acagatggaa tttgggtata gaaaaagtat aatatcgaaa aacggatata
      721 tagctttatc agcagaatta gaattacaag aaggaaatta tgatgaaata aaaagtttaa
      781 tggatgattt agcgacgaga agaataacta aacagccatt aaattttgct agtgcaggaa
      841 gtacatttaa gagaccaaca ggatattttg caggtaaatt aatagaagaa actggattga
      901 gaggtcttac tttaagagga gcacaagtat ctgagaagca ttgtggattt gtagtcaatc
      961 aaggagaagc aagtgctaaa gatattttgg atttaatata tgttataaaa agtgctgtat
     1021 atgccaagtt tggggttatg ttggaagaag aagtcaaaat acttggagag gattaacaaa
     1081 tgaaaatact ggctgattat catacacata cattatatag tcatggcaaa gggactattg
     1141 aggataatgt aaaggtagcc atttctaaag gaataaaaac aataggaata tctgaccata
     1201 gttataaaca tattgcatat ggtgtaaaga aaaaagatat tctcaaaatg agagaagaag
     1261 tagattattt aaatgataag tacactgata taaatatact tttaggtatg gagtgtaaca
     1321 tattagatga taaaggaaat atagatatgg atgataaatt tattagagat tttgattata
     1381 ttatggctgg atatcatttt ggttctactc caacatcatt aagaagctta ttaaatcatt
     1441 gtaataacta tatttttaaa actgaaaaag caaaagaata taatacaaaa gctgtaataa
     1501 atgcgataag taataatgat atttttataa ttactcatcc aggagataaa ggcgatgtgt
     1561 atatagaaga agtagcaaag acagcaaaaa agacaaatac tagacttgag ataaatagta
     1621 gccattcttt tttgaatgta aatcaattaa ataaaataaa agggctagaa aataaattta
     1681 taataggctc agatgcacat tgtccagaaa gagttggtga ttttgaacta gctatgaaga
     1741 ctatatctga ctctaatctg gatatatcgt tagtagaaaa cattataata taataaacat
     1801 aagataaaag ggaggagtta gcaatgaaat ttgttatagt tacaggtctt tcaggatctg
     1861 gaaaaagtga gacaatgaga gctttagagg atatgggatt ttattgtgta gataatctac
     1921 ctcctgcttt gataactaaa tttgcagaat tatgttatca accaaattca agtattgata
     1981 aagttgctct agggatagat ataagaggaa gaaagttttt tgaagcgctt catgaaagct
     2041 taaattattt agaaaaagaa aattatgaat atgaaatggt atatttggat tgtaatgatg
     2101 atgtattgct aaagagatat aaaatgacaa gaagaaatca tccattggca aaagatatgc
     2161 agattccaga aggtataaaa atggaaagaa agataatgga acctttaaag gagttatcta
     2221 catgtataat tgatacaact aacatgaaac ctaaagacct taaggaagaa ataaagaaaa
     2281 tatattcttc tggtgaagat aatccaaacc taactatttc tgtagtatct tttggattta
     2341 aacatggtat acttgctgat gcagatttag tatttgatgt aagatttctt ccaaatccct
     2401 attatgttga agagttaaga gctaaaactg gtgatgataa agaagttaga gattatgtta
     2461 tgaattctaa aatcagtgaa gaattttatg tgaaactttt agatatgatt cattttttag
     2521 tacctcaata tatagaggaa ggaaaacaac atctagtaat tggtgttgga tgcacaggtg
     2581 gaagacatag gtcagttact ataacaaatc ttatagcgga ggatttatct aataagggat
     2641 atagagttgt aaagaaacat agagactcta tgttgcgata atggaggcaa acatggctaa
     2701 attacttatt attgtaggaa ttttaggttg gattgcatta attgtatcgt tatttatata
     2761 taaaaaagca aaaaatgaaa aagtaaaact atttcaaagg tctattgttg aaaatagaga
     2821 accaaatgtg gttgtgatag gtggaggtac tggtcaatcg gtatttttaa gaggtcttaa
     2881 acacactacg caaaatataa ctgcaattgt aactgttgct gatgatggtg gaggttcagg
     2941 tgtattgaga gaagacttag gaatgctccc accaggagac ataagaaact gtttattagc
     3001 acttgcaaat atagagccaa caatgaatga agttatgcaa tataggttta cagaagggct
     3061 tttaaaaggt cagagttttg gaaatttgtt tttggctgct atgaatgggt tgtatggaaa
     3121 ctttgaaaaa gctgtatata aattaagtga aatttttgct ataacaggaa gagttttacc
     3181 agtaactttg gaagatgtaa atttagttgc caaattagaa aatggcaata taataaatgg
     3241 agaatctagt atacctgaag agtcaaaaat tcaaaaaagc tctattgata aaattttttt
     3301 aaatccaaaa gatgtaaaac cattaaagga tgttattgca tctatttatg atgctgacat
     3361 tataattatg ggtcctggaa gtttatatac aagtataata ccaaatttac ttgtagaggg
     3421 gattgtagac gctattaaat cgtctgtagc tccaaaagta tatatagcaa atataatgac
     3481 tcaaccaggt gagacagaag ggtacaatgt attagagcat gttaatgcta tagtaaaaca
     3541 cactgatgag aatttaatag attatgtaat cgcaaataat gagatattgc cagaagaaat
     3601 gcttgactta tataaacaag atggagcaga gcaagtcttg ttagataaaa agcaaaaaga
     3661 taaattaaaa gagatgggta taaaaactgt agagaagaat ttaattgaaa taaaaaataa
     3721 ttatataaga catgatgcaa agtatatatc taacatagtt attgagttag ccctaaatca
     3781 taattacaac aaaagttagt aaaaaaggat tttaaaaaat tgtgtagaaa tccttatcat
     3841 tatgttagtt ttaaggagtg aacatcgatg agagaagagg ttagtgctgg tggtgtagtc
     3901 ctgtttggca atacgattct tttactaagg aagttcaatg gagattgggt gttacccaag
     3961 ggaaaagtag aagaagggga aaataaccaa gaggcagcat tacgtgaagt aagtgaagaa
     4021 actggagtaa aagcagatat attgaaatat ttaggtgaaa tacactatac attcaaagaa
     4081 aactgggatg aaaatagagc agttcataaa acagtttttt ggtatttgat gcaagcaaag
     4141 aatatggata ctattccaca aaaagaggaa ggctttattg atgctaaatt tatccaccta
     4201 gatagagtag tagacttagc taggtatgat gatgaaaagg agataataaa ggttgcctta
     4261 caagaaataa aaaaaaggtt aaagaaaaat tagttaatag gtgatagata tgtctttttc
     4321 aactgaaacc aaaaatgaac tagcaagagt tatatctggg aatgaatgtt gtaatatagc
     4381 tgaattgtca gctctagtta aatcaggggg aagtatacaa attgttggat ataagaagct
     4441 aaatctaaag ataacaacag agttaaattc aatagcccgt aaagtattta aattattaaa
     4501 aaagaatttt aatatcaata ctacaatctc agtaaataag aatcaaatgc taaagagaaa
     4561 taacagttat gtattaatgg taacaagcga aatgggttca gaaatgttat taaaaaagtt
     4621 aggtatactt gaagataaag aaggattttt tactataaac aaaatttcag agaacttaat
     4681 taagcatgaa ggatgtaaga gagctttcat aagaggagct tttcttggtg gaggttctat
     4741 aagcgaccca gaaaagaact atcatatgga atttgtaact aataatgagg agtttgcaga
     4801 ttctctaaaa gaacttataa actctttagg ttttaatagt aaaattgtgg ctagaaaaaa
     4861 taatcacgtt gtttatatca aagagagtga acaaatatca gatttgttga gtattatagg
     4921 aggacaccac gctcttttaa gtcttcaaaa tactaaaata gtaaaagaaa tgagaaataa
     4981 tgtaaacagg atagtaaatt gtgagacagc taacctttct aaaacagtaa atgcagcagt
     5041 aagacaagta gaaaatatta agctcataca agagactata ggaattagca gtttgccaga
     5101 aaatttacaa gagatagcta agattagaat cgagtatgag gatatgactc tcaaagaact
     5161 tggagagatg ttagaaccac caataggaaa atctggagtc aatcatagac ttagaaaaat
     5221 agaagaaatt gcaacagatt tgaaaaaaaa atcatagcaa taaattgagg ggacaatctc
     5281 agaataagca ttaatttata acatataatg gtatacgttg aagttaattc ctattttgag
     5341 ataaaagtct ctgtttttgt tttaggacat atatttgtgg taaaattaaa attataaagc
     5401 aggaggtgat ttaatgaaca aagattttgc tcatcttcat gtacatactg agtatagttt
     5461 acttgatggg ttttcgaggg tgaaaaaact tataaaaagg gctaaggaac taaacatgag
     5521 ctcaatagct ataactgacc atggttgtat gtttggtgtt atagatttct acaaaacagc
     5581 tataaaagaa ggaataaagc caattatagg gtgtgaagtc tatacagctg cacgtggatt
     5641 gagagataaa gacccaaact atgacaaata tcaaggacat ttagttctgt tagctaagaa
     5701 tatggaagga tataaaaatt taataaaaat agtttcaact tcatacgtag aaggttttta
     5761 ctataagcct agagttgaca tggaagaact taaaaaacat aacaaaggga ttatagcact
     5821 ttctgcatgc cttgcaggag atgtagcacg tgctttaatg aatagaaatt atgaaaaagc
     5881 aaaacaattt gctcttgact acagagacat atttggagaa gaaaatttct ttttggagat
     5941 tcaagatcac aatttacctg agcaaaaaga agtaaactct ggtttagtaa aattatcaaa
     6001 agagactggt ataccactag ttgctactaa tgatgttcac tatgtaaata aagaagattc
     6061 gaaaatacat gatgtactta tgtgtattca aatgggaaaa acattaaatg acccaaatag
     6121 aatgagattt ggtagtgatg agttttattt aaaatcaaga gaagaaatgg aagaattatt
     6181 tccatatgca ttagaggcta ttgacaatac tgtgaaaata gctgatatgt gtaatgtgga
     6241 gtttgatttt aatactatac atcttcctaa gtatgatgtt ccagaaggat atacaccaga
     6301 aacttattta agggagttgt gttttaatgg attaaaagag agatatgata atcctagtaa
     6361 tgagatacta gagagattaa attatgaatt agatgtaatt gaaaaaatgg gttatgtaga
     6421 gtatttttta attgtttggg attttataaa tttttctaaa gaaaataata ttatagtagg
     6481 accaggtaga ggtagtgcgg ctggttcgat agtagcatat acacttaaaa taactgatat
     6541 agacccaata aagtactctc tattatttga gcgtttttta aatccagaac gtatatccat
     6601 gccagatata gatatagatt tttgttatga gagaagagaa gaagttatag attatgtaaa
     6661 gagaaaatat ggtgatgacc atgtagccca gataataact tttggaacaa tgggtgcaaa
     6721 agctgcaata agagatgttg gaagggtatt ggatataggc tacaataaag ttgacaaggt
     6781 agccaaggaa ataccatttg ctttaggaat gacaatagat aaggccctag atacaaatcc
     6841 taatcttaaa gaattgtatg accaagaccc ggaaacaaaa gaaataataa atatatcaaa
     6901 acagatagag ggaatgctaa gacatgcttc tactcatgca gcaggagttg ttatatcaaa
     6961 aaatcccgta gatgaatatg tacctttgta caaacatcaa gatgcaatca caacacagtt
     7021 tactatgaca acacttgaag aacttgggct tttaaagatg gattttcttg ggctaagaac
     7081 tcttacagtt ataagagata ctcttgattt aatagaaaaa aatagagtta taaaaaatta
     7141 cacagaaaaa atagactttt caaagatgga ttatgacgac ccgaaagtat atgaaatgct
     7201 ttcatcagga aatacactag gggtgtttca attggagagt gctggcatga gaagttttat
     7261 gaaacagcta aaaccagaca attttgaaga tattgtagcg ggaatatccc tatttagacc
     7321 aggtccaatg gattctatac ctgcatatat aaaaaataaa agcaacccaa aagatgtaac
     7381 atatctacat gaaaaattaa agcctataat ggaagttaca tatggttgtt tggtatatca
     7441 agagcaggtt atgcaggtcg ttagagattt aggtggttat agttacggac gaagtgacct
     7501 agttagaaga gccatgagta agaaaaagat ggacgtgatg gaagaagaaa gacaatattt
     7561 tattcatggt aaatttgatg aagatggtaa tatagaaatt gcagggtgta tcagaaatgg
     7621 tgtagaagag gaaatagcta ataaaatatt tgatgatatg atagacttcg ctagatatgc
     7681 atttaataaa tcacatgcag ctgcatatgg ggttttagca tatgagacag cttatctaaa
     7741 aacatattat ccagttgaat ttatggcagc attgataacc agtgtaatgg gaaatactga
     7801 taaggtagtt gagtatataa gagaatgtaa agcactagaa atagatgtat tacctccaga
     7861 tataaacaaa agtttttcaa agttttcagt tgaaggcgac aatataagat ttggtctagc
     7921 agccgtaaaa aatgttggcg taaatataat aaacaatata atcgctgaaa gagaatctaa
     7981 tggattattt gtagatttag tagatttagt caaaagatta gaccaaaaag atacaaataa
     8041 gagagtaata gaaagtctaa taaaatgtgg agcttttgat aatataagtg aaaatagagc
     8101 tagtctgatg gctggttatg aaagtttatt agaaagtgta tctatggata gaaagaaaaa
     8161 tgttcaaggt caaatttctt tatttgatgc ttttggagat agtgaggaag atagtgattt
     8221 tcaacaaata tacagtttgc ctaaaaggca agagtacgaa gaaagagaga gacttaacct
     8281 tgaaaaagaa gtcttgggaa tgtatgtaag tggacatcct ttatcacaat ttgaaaaaga
     8341 actacaagaa aaaacatcta tggataatgg aaaattaaat tcattaaaag aggaagaaga
     8401 gtcataccta caaatgaatg aaacagatgt tataatgggt ggaatgattg taactaaaac
     8461 tattaaaaca acaaaacgaa atgaaattat ggcatttatt gaattagaag atttgtatgg
     8521 tgctatagaa ataatagttt ttcctcaatt attacaaaag tataatgtca ttttgaatga
     8581 agataatata atatatgtaa aaggaacatt aagtataaaa gaaggcgaaa atgcaaaact
     8641 tatagctaga gagataaaag atataaatga tgatactgac tttgcagata gaaactacaa
     8701 ttcaggaaga actagaaatg tagaaaatat taagccagaa agtggtaaaa aattgtattt
     8761 gaaaatagat agtatgtctg atactgatac aactaataat attgtagaaa tagccaaaca
     8821 atatccaggt aatgacagta tatacttata ccctatggat gaaaatataa atggtaaacg
     8881 taaagcatat aaaatgaccg gggcattgac ttgtatatgt gatgaattta taaataactt
     8941 agaagagtta cttcctaaag ataacataaa agtaaaagct tgatttttta tataaaaatc
     9001 taaatatatt ataataatat gaaaaatatt gtccgttttg tgatataata tcacaaaatg
     9061 gacatttaag gacatttaat ttttttagtc ttgatgggat taaaaagtga tatagggaca
     9121 aaaaaagtcc attagaatac aataacaatg attataataa aaaattgaca taaaatccaa
     9181 ggaggtaaaa aatgaaaact ataggattac taacaagtgg aggagatgct ccaggaatga
     9241 atgctgcgat tagagcggtt gtaagatcag caatatatta tggatgcaaa gtatatggaa
     9301 tcaatagagg atataaggga ttactagaag aagatttaac tgaaatgaac ttatcttctg
     9361 ttggagatat tatacacagg ggaggaacta tattaaaatc ttccagatgt gaagaattta
     9421 aaacagaaga aggtagatta aaagcagtaa aaattcttaa aaagtataaa attgattgct
     9481 tagttgtaat tggtggagat ggttcatttg caggagccca aaaacttagt gacttaggat
     9541 tcccagctat aggcatacca ggaactatag ataacgattt agcatataca gattatacta
     9601 taggatttga tactgcaatg aatactatta tagatgctat aggtaagata agagatacat
     9661 catcatctca tgaaagagtt aacatagtag aagttatggg aagacactgt ggagatttag
     9721 ccttatatgc aggtcttgca ggaggagcgg aaactataat agtaccagaa gtagaaatta
     9781 cagtagatga agttgctcta agattaaaga ctactcaaaa aagaggtaag agacatagta
     9841 taatcgtttt agcagaagga gtcggaagtg ctagtgattt agaaaaggaa cttaaaaaag
     9901 agtcaggagc agatttaaga gtcactgtat tggggcatgt ccaaagagga ggaagcccaa
     9961 ctgtatctga tagaatacta gcaagtagac ttggagtaag agcagtagaa ttattattag
    10021 atggaaaatc agctagagtt gtaggaataa aagaaaacaa aataattgat ttagaaataa
    10081 gtgaagcttt agctcaaaag aaagtatttg ataaagaagc ttatgagatg gcaaaaatat
    10141 tatctatata agttttagta ttaaaaataa atctgtggaa taatatctca ggaggagcaa
    10201 atatgttaaa taatgtaaag aagacaaaaa ttgtttgtac actaggtcca gcatcacaaa
    10261 gtgaggaagt tttaactcaa cttatgcaaa atggtctaaa tgtgtgtaga tttaactttt
    10321 ctcacggttc gcatgaagaa cataaagaaa gaattgatat ggcaaagaaa gttagagaaa
    10381 aattaaataa gccagttgct atattattag atactaaagg tccagaaatt agaacaggta
    10441 actttgaaga cccagaggta tttttagaag aagggcaaaa gttcactata actatgaaag
    10501 atattatggg gactaaagaa atgtgtacag taagctataa aggtcttgct gaagacgtta
    10561 aatcaggaga ttctatacta atagatgatg gtttagttgg cttaagagta aaagaaataa
    10621 atggagaaga tatagtttgt gttgttgaaa attcaggaat agttaaaaat cataaaggag
    10681 taaatgtacc tggtgttaag ataaatttac cagcaataac tcctaaagat ataagtgata
    10741 tagagtttgg aataagtcaa ggaatagatt atatagctgc ttcttttgta agaaaggcat
    10801 ctgatgtact agcaataaga gaagtattag agaacaataa tgcaactgat atacaaataa
    10861 tatctaaaat agaaaaccaa gaaggtgtag agaacctaga tgaaatatta aaagtttctg
    10921 atggtataat ggtggctaga ggtgacttag gtgttgagat accaactgaa gagatgccaa
    10981 tagttcaaaa aatgatgata aaaaaatgta atgaattagc aaagccagtt gttactgcca
    11041 ctcaaatgct tgattctatg ataagaaatc caagaccaac aagagcagaa gttacagacg
    11101 ttgccaatgc tatatatgat ggaacagatg cgataatgtt atctggagaa actgctgctg
    11161 gaaaatatcc tgtagaggca gtaaaaatga tggctacaat agcaaaaaga acagaagaga
    11221 ctttagatta tgatagatta ctaaaggaaa atggtactaa caatgttact gtaacagatg
    11281 caataagcca tgcaacttgt acaacagcag tagatttaaa tgcatcagct ataataacat
    11341 ctacttcttc aggatatact gctagaatgg tatctaaatt cagaccaaaa tctccaataa
    11401 tagctactac aaataatgaa aagactatga ataaattagc tcttacatgg ggagtatatc
    11461 cagttaagtc ttctgttgca ggaaatacag atgaagttat agaaaaggca attgaggcag
    11521 ctagacaagc tgattatata gataatgggg aattagtagt tataactgct ggagtaccag
    11581 taggagtaag tggaactact aatttaataa aggttcatgt aataagcgaa gaaatcgtgc
    11641 aaggtatagg tgtaggtact caaacagtag aaggtaaagt tcgtataata aagaatggaa
    11701 atgcttgtgt agactttaat gaaggcgaca tacttgtttc tactgctact gatgctgaaa
    11761 tgaacaatta tatagaaaaa gcaggagctg tagtaactga gaatggtgga atgacatcac
    11821 atacagctat tgtaggtata aatttagata taccagttat tgtgtctgca gctgatataa
    11881 caaatctagt aaaagatgga gaagttgtaa ctgtggatgc atcaagaggc gtggtatata
    11941 gaggttcaac tagagtttta taagaaaaat aataaattta tttactaagg ggctttataa
    12001 gctccttttt ttttggtaaa ataaaatatt gtagcttagt aaaaaatata gtttaattaa
    12061 tagcaatatt ttaatataaa tttaaagaaa aaagtttgta aagatttaaa tataatagat
    12121 tgattggagg gtgatttatg ctttcgaaaa acgagatgta tattgtagat atagtagaca
    12181 ttggtcaagg cggagtagga gtaggaaagt acaatggtat tacagttttt gtagagggcg
    12241 gattaataca tgataaagtt aaagttagga ttaataaagt aaagaaaaat tatgctgtgg
    12301 gagatattgt tgagataata gaaaagtctc cattcagggt gaaaagagtg tgtagtgaca
    12361 atcttaggga ttgtggaggg tgtcagatac aagacttaga ttataataaa caacttgaaa
    12421 tgaaaacaaa tgaagttaaa caagtacttt cgagaatagg taagttagag gatgtagaag
    12481 ttcatgacac tataggaatg gataatccat atagatatag aaataaagca caatttccaa
    12541 tacaaaagaa taatggtatt ccaattattg gcttctataa aaagaagact catgatgtaa
    12601 tatcgacaga aaaatgcgta atacaacatg agataaatga taaaattgtt aaaataataa
    12661 aaacatatat aagggcctat aaggtaagca tatatgatga aaagactcac acggggttga
    12721 ttaggcattt agtaacaaaa ataggtttta caactaaaga agtaatggtt gtattagtag
    12781 caaatggaaa caaacttcca tatcttaaag aattagcttc agtgcttaag gaaaatatac
    12841 ctggatttaa gactttggtt gtaaatgtaa atcaaaaaga tacgaatgct attctaggaa
    12901 gagaaaatat agttatatat ggagatggaa aaataaacga ttatatagga gacttagtgt
    12961 ttgagatttc accattatca tttttccaag taaaccctgt ccaaactaaa gtattgtatg
    13021 ataaggctct tgaatatgca aatttaaatg atactgatac tgtttttgat atttactgtg
    13081 ggataggtac aatatcttta tttttggcac aaaaggctaa aaaggtttat ggaatagaaa
    13141 ttatagatga tgccataaaa gatgctaaga taaatgctaa attaaacaat atacataatg
    13201 ctgaatttta tgttggaaag gcagaggaaa ttgtacctaa gatgtataga gagggtaaga
    13261 ctgccaatgt agtcgttgta gacccaccta gaaagggatg tgacgaaaag gtattagaga
    13321 ctattgtttc aatgaatccg gaaagaattg tctatgtttc ttgtaatcct tctactttag
    13381 caagggattt agattatcta aatgttcacg gatttaaatg tgttgaggca caacctgtag
    13441 atatgttccc tcattctaca catgtggaaa ctgtagctaa gataatcaaa caatagtaat
    13501 gtttatatag attatatact aataatatat tgtatttgcc accgatttgc cactgtaaag
    13561 cgatttcggt ggcaaattta gtctacaatg ttatcaaaaa tctctaaagt tttattttta
    13621 attctataaa acttattata attgtaattt taaaagaaat agtcaatcta cagtagccag
    13681 attttataca actcattttt ttcatagagt aatttacata atctatagtt gaatattatt
    13741 taatattttt ataattcatt agcaaatata ggcattacaa atgttgataa acatgtgaaa
    13801 gcacatatcc acttataatt atttttttta aaaccataaa atcacctctt catttgttgt
    13861 atgtttttat aacaaatgaa gaggtgattt ccagacaatt taaaaggttt ttgtatattg
    13921 actaaaattt atttttatta tttataattc ttaataatac atatgtacct actgattcca
    13981 tattaaactg tataattttt gttcatcttt ttttataatt aaaactacgt agttctcata
    14041 atttctctta tatatatact tatcatcttt tttaacttct aacttatatt ttttaaaatc
    14101 ttcttcaatt ttatttttat tacttttatt aatgttaaca gaatctcttt taaatttatc
    14161 aatatatttt tctatttcat taatattatc tttgcttaca actataccac cttttaaatt
    14221 aaaaactttt ttgaaatttt tttcagaaaa tccttgaata aaataacttt gtccatctcc
    14281 attaatacta tcttgagtat caaatacttc aatttctttg ttaggtattg gtatatcaaa
    14341 attccaagat tcctttagca ttgaaatatt agctgttttt cctaaaaaaa gattatatag
    14401 gatacttcct aataacaaaa cacctattaa tagtagtgtg attaatacaa tttttctctt
    14461 tttcattttt cgttcacaac cttttgaaaa aacatgattt aattcatcat ttgaaggcat
    14521 gtcttcaaga ataatttttg ttgtgatagg aatgctttct tgaaacatat catttgtaat
    14581 atcaaaatct tttctttgat tactcaatat ccatttcttc taatatttct tttgattttt
    14641 tctagtacat tgaatactct tatatacatt ctcctctttt aaaattaaaa gttataaaag
    14701 aataagttat ataaaaaatt attatatttt ttaatatagc ttatttgcaa atttataatt
    14761 ttgttaatct aatccttcaa actaaaaaat catttatatt tacagaattt cattttaa
//
LOCUS       DAYATN010000098        14678 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.098, whole genome shotgun sequence.
ACCESSION   DAYATN010000098 DAYATN010000000
VERSION     DAYATN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.098"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(66..245)
                     /locus_tag="ACGI8A_002672"
     CDS             complement(66..245)
                     /locus_tag="ACGI8A_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180663.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(427..633)
                     /locus_tag="ACGI8A_002673"
     CDS             complement(427..633)
                     /locus_tag="ACGI8A_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180664.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(681..1106)
                     /locus_tag="ACGI8A_002674"
     CDS             complement(681..1106)
                     /locus_tag="ACGI8A_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180665.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            1278..1505
                     /gene="acpP"
                     /locus_tag="ACGI8A_002675"
     CDS             1278..1505
                     /gene="acpP"
                     /locus_tag="ACGI8A_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFH0180666.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(1649..2653)
                     /locus_tag="ACGI8A_002676"
     CDS             complement(1649..2653)
                     /locus_tag="ACGI8A_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFH0180667.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(2667..3569)
                     /locus_tag="ACGI8A_002677"
     CDS             complement(2667..3569)
                     /locus_tag="ACGI8A_002677"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFH0180668.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(3764..4939)
                     /locus_tag="ACGI8A_002678"
     CDS             complement(3764..4939)
                     /locus_tag="ACGI8A_002678"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFH0180669.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(5026..5871)
                     /locus_tag="ACGI8A_002679"
     CDS             complement(5026..5871)
                     /locus_tag="ACGI8A_002679"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HFH0180670.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(5969..6766)
                     /locus_tag="ACGI8A_002680"
     CDS             complement(5969..6766)
                     /locus_tag="ACGI8A_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFH0180671.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(6785..7795)
                     /locus_tag="ACGI8A_002681"
     CDS             complement(6785..7795)
                     /locus_tag="ACGI8A_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFH0180672.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(7814..8596)
                     /locus_tag="ACGI8A_002682"
     CDS             complement(7814..8596)
                     /locus_tag="ACGI8A_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFH0180673.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(8655..9791)
                     /locus_tag="ACGI8A_002683"
     CDS             complement(8655..9791)
                     /locus_tag="ACGI8A_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFH0180674.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(10500..10967)
                     /locus_tag="ACGI8A_002684"
     CDS             complement(10500..10967)
                     /locus_tag="ACGI8A_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFH0180675.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(11046..11588)
                     /locus_tag="ACGI8A_002685"
     CDS             complement(11046..11588)
                     /locus_tag="ACGI8A_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180676.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(11640..12326)
                     /locus_tag="ACGI8A_002686"
     CDS             complement(11640..12326)
                     /locus_tag="ACGI8A_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFH0180677.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(12326..13186)
                     /locus_tag="ACGI8A_002687"
     CDS             complement(12326..13186)
                     /locus_tag="ACGI8A_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180678.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            13321..14121
                     /locus_tag="ACGI8A_002688"
     CDS             13321..14121
                     /locus_tag="ACGI8A_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HFH0180679.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            14146..>14678
                     /gene="yfcE"
                     /locus_tag="ACGI8A_002689"
     CDS             14146..>14678
                     /gene="yfcE"
                     /locus_tag="ACGI8A_002689"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFH0180680.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLI"
BASE COUNT         4675 a         2669 c         1608 g         5726 t
ORIGIN      
        1 catccttctt ctccaaaaaa tctatctaac ataacaaagc cctccttttt ttaataactg
       61 tgttcttatt ctaagcatcc accacagcag caaagtatta ataaaactat taggaatggt
      121 atccatgcca ttatgtcaaa ttctgcccag ctatctccaa aagctaagaa taggaagaat
      181 agtactatta tccaccatcc tcctccacca aaacatcctt cttctccaaa gaatctatct
      241 aacattatac gttacctcct gttgatattt tgttgagctt ttcgctccta acatatacta
      301 tgcatttggt ttccattttg ctaatgtatt tttactattt ttttaaattt tttaatttga
      361 actaaaaaag gtaaaaccat aacttgtata agttactggt tttacctatt taataatcaa
      421 tattttttag caacaacaac catcatcgaa gttaaataat aacaatacta ttaatataac
      481 ccatatccat atattgtcat cgcataataa gtcttctaaa atgtctgtgc ataatagtaa
      541 gaatactata actattatta tcaacatttt attcccaaaa aatccttcgc atcctccaaa
      601 gaaacgttcc atattgtctt ctaattttgt cataactaat cctccttgaa tcattaagag
      661 actaagtttt gtctctttaa ttaaatatat gtagtaatag acagataagt tacttctaaa
      721 aagtttttat tgattagttt atttatttct ttttcaccaa gtttattgta tctatcaact
      781 tcatccagat aatctttttt ctcctctgat atagtataga ttaaactttc tattgatttt
      841 attttgtact ccttatatct ttctatcaag aaggatgttt ttgaattttc tattcctttc
      901 tttacatgca ttaaccttga tatgtagtct tctttttcat tagatacata actctccttt
      961 tcagctacat cttcaaggat taaattggtc tccattacat aatcctctat acgttgcata
     1021 tctaaagtta ttgaattata ataaaaattt actgctataa gtaaaaacat tcctactgaa
     1081 gttagtaata gtcctacata tttcatagca tttcctccta agttatattt gttatcaata
     1141 aagtttattc atcaaataat actttattac tacatttata aaattttatt attttttata
     1201 gattccatat tgaaaaattc ttgatattat attaaactaa cattaaaatt aattaaaact
     1261 gctttaggag gcttaaaatg tttgagaaaa taaaagaaat aatagcagaa caattagggg
     1321 tagacaattt agatgagata actatggaag catctttaat ggacgactta gaagctgatt
     1381 cattagatgc tgttgaaatt ataatggccc ttgaagatga atttggaata gaaataccag
     1441 acgaggaagc tgagaacttt aaatgtattg gtgatatatg taaatatatc gaagaaaaca
     1501 aatagtatcg ttttgtctac aaatataaat agtctgtgta aacttctaca taataaatta
     1561 tattagagtt cgcacagact agattacatt tatttacaat atataaaata cacgctctta
     1621 tatttaattt atatactaag tatataaatt acttttcttc ataaaacgta acattcttta
     1681 ttattatatc tatagttcca tcttgatttt gttttacagt atacttcatt gggtcttcaa
     1741 aatcagttag attccctttt atgtcaaaac cattatccgt ttttatactt ctttttttaa
     1801 gctttttctc aacccatttt ttatctatac taaaactttc gtcaagacct ttttcttcca
     1861 tatgctcttt aaagctatcc tttaactcat catctttaat tgaattatca acaaagtcat
     1921 ttatatcaac ttcatgtttt tccttcagtg tataatttaa tatacttctt acatcttcag
     1981 cttgctttat atcattacta agagcattgg ttatccaatt ttcagctgta tttttaaata
     2041 ccttggtctt gtacttatca tcatctattt ttttagcatt taaaaattct gttacaaact
     2101 tagattctga cccttctttt tcagcatcct tatctaaaag ccttagatga aactcatcat
     2161 ttataccact tagactaatc aaagctcctt gcttttgtct taaagtctct gggataccaa
     2221 tttcatttga aaccatttga atattaaatt tatcatccac aaattcaatt gaatgagtgt
     2281 acaacttttt ataatctaat ttcagtattg ctacactctt ttcatcttta atagtgtata
     2341 aacaaattgc caaatcacaa gattctagtg cagcattaat tttcataacg tcaaacaagt
     2401 aagatgcaat ttctttagaa tttttcaaaa aagtactttc atcatatatt atttgttcac
     2461 aacagttttt aattaaattg tcattataat ctttaaaaac accttttctt aaatcctcat
     2521 cttttgcaat tctttttata gctttctgga aaaaaccatc cacttcttga ttaactttgc
     2581 cttcaaagtc atttaatatt ggaacatcac tatttttgtc caaaacatgt attataaatt
     2641 tatgtattat catatatatc accccattat cttaatttcg atattaactc tttcatatcc
     2701 tctggtatat ctgctttaaa tttcaatttt tcttttgttc ttggttgttc aaactctagc
     2761 ttatatgcat gaagtgcttg cctatttatt agctcttcat ctacataacc atacaactca
     2821 tcacctatta ttccatgacc tatatgagcc atatgtactc ttatttgatg tgttctacca
     2881 gtttctaagc ttacttctaa tacagttgca ttttctagtc tttctattac tttatagtga
     2941 gtaacagatg actgtcctct ttcatctatt atcctcttta ttgaatcttc tgtaggtcta
     3001 taaattggct catctatagt tccctcattt tcttttataa ttccatcaac tactgttata
     3061 tacttttttt caactttatt ttcactcatt gctgtagaca aagtatgatg tgcataagca
     3121 ttttttgcta ctataactaa ccctgaagta ttcatatcta acctatttac aaatcttatt
     3181 tttacgtttt ctttttgatt atctatatag taactaatac catttgctat tgttttatca
     3241 tagtggcttt ttgttgggtg aactaccata aacggaggtt tattcaccat aattatatca
     3301 aaatcatcat aaattatctg taaatttaaa tcttgaggtt caaaatttgc tttttcctcg
     3361 tctattttta cttctattaa atcaccacta tacaccttta aactcggttt tttatatact
     3421 ccatttacta aaacactctt ttccctcttc atttttgata atgacctaac tgagaaattt
     3481 agcttgtcca ataaaacttc ttttaaagtc atttcttctt catttgtata cgatataaga
     3541 ttatatcttt gattctcttt tttaaacaaa cttttaccta ctttctttta tatcttccta
     3601 ctttttatat cttcaaaatc atgatattat atattttaca ctatttttgt ttaatagata
     3661 ataattattt ttaactataa aaacctgtaa gaataacaaa tagttattct tacaggttaa
     3721 attcataatt aaatatttaa tttgtaaata ttataatcta aaactatctc ttaactatta
     3781 aagtagttcc cattcctcca cctatacaaa gtgtagcaag accagtttta gcatctcttc
     3841 tcttcatttc atataaaagt gtagtaagta ttcttgctcc tgagcatcct attggatgtc
     3901 ctatagctat tgctccacca ttaacattaa ctttattcat atctatattt aagtctctta
     3961 ttacagctac agattgggca gcaaatgcct cattagcttc aactaaatct atatcttcaa
     4021 tagtcatatt agcagcttct aaagcttttt tagttgctgg aactggtcca tatcccatta
     4081 ttttagggtc aacaccagct gttccataag aaactatagt tgcaagaggc tctattccta
     4141 gttcttcagc tttttcttta gccattacta ctagcatagc agcaccatca tttattcctg
     4201 atgcattacc agcagtaact gttccatctt ttttaaatgc aggtcttaac ttagcaagtt
     4261 tctccattgt agtgccaggc ttaatatatt catctttatc tactacagtg tcaccttttc
     4321 ttccttttat aacaacagga actatttctt catcaaattt tccttcagct tgagcttttt
     4381 cagctttatt ttgacttgca agagctaatt catcttgttc ttctctagtt atattccatt
     4441 gctctgctat gttttcagca gtaataccca tgtgatagtt attaaatatg tctgataatc
     4501 catcttttat cattgaatca acaaaagcag catcacccat tcttgcacca tatctcgcac
     4561 ttggtactaa ataaggagac atactcatgt tttcagctcc accaactaac attatatcag
     4621 catcacctaa tgctataagt tgagatgcca ttgaaacaga tcttaatcca gaaccacaaa
     4681 ctatatttat agtcatagct ggtttttcta ctggtattcc tgctcctaat gctatttgtc
     4741 ttgctatatt ttgtccaaga cctgctgtaa gtactccccc taaaagagat tcatctatca
     4801 tatctggagt tatgttagct ctttttatag cttctttagc tgctgttacc cctaactcta
     4861 ccgctgaaac tgatttaaat gctcctccaa aacttcctac tgctgttcta gctgcactgg
     4921 caattactac ttctctcata ttcattaccc ccattaaatt agatttttca tatatccgaa
     4981 attgtttttt aaacaatttt atttaaactt ttaattccca aattcttatt tattataatc
     5041 atagaatcct atcttagttt ttcttcctaa ttgattagct ctaaccattt tagctaaaag
     5101 tggatgaggt ctatatttag tatctccaaa ttcagtatat aaaacgttca ttatagctaa
     5161 aacaacatct aatccgatta aatcacctaa tgctagtggt cccattggat ggtttgctcc
     5221 taatttcata gcttcatcta tttcttcttt acttgcaaca ccatctgcat atataccaac
     5281 agcttcattt atcataggta taagtattct atttactaca aatccaggag attcagatac
     5341 atctactggt actttattga tactcttaga taattcaaat actgtatcaa aagtaacttt
     5401 tgatgttaac tgaccactta taacttcaac taatttcatc ataggaactg gattaaagaa
     5461 atgcattcct ataactttat ctggtctctt agtagaagaa gctatttctg ttatagataa
     5521 tgatgaagta tttgttgcca agatagtatc ttctttacat aattcatcta gtaacttgaa
     5581 aacatctttc tttatattca tgtcttctac agatgcttct attattaaat ccatatcttt
     5641 taaatcttca tagttagtag ttgaactaac atgacttaat atttctgctt ttgtagcttc
     5701 atccattttt cccttagtaa ctaacttagt taaattttta tctaataaag ctaaacattt
     5761 atctatagca ccttgagttc tactctttaa acatacatca tgtccacaac ttgcaaaagt
     5821 ttgtacaata ccacttccca tagttccact acctattaca gctaatttca tagttataac
     5881 ctcctgttat tttagtattt atattctgtt aactagatta aaatactatt taatctagtt
     5941 aacagcaaat atatttttaa ttagattttt acccttttat aaagttagct tctctctttt
     6001 caacgaaagc tgacattcct tctttttggt ctttagttga aaaacaaaga ccaaataaat
     6061 tagattctat atctattcca gtatttatat cagtttgagc accaagttgt attgcttctt
     6121 tagagtatct aactgcaagc tgagcatttt tagctattat cttagctaat ttctcaactt
     6181 cttctattaa aatgtctggc tcaacgactc tatttactag ccctattttt tcagcttcat
     6241 cagcttttat aacttgacct gtaaagatta attcttttgc ttttgccatt ccaaccaatc
     6301 ttgtaagtct ttgagttcct ccatatcctg gagttattcc aagagttact tctggctgac
     6361 caaatttagc tttagcagat gcaattctta tatcacatgc cattgcaagt tcacatcctc
     6421 cacctaaagc aaatccgttt acagcagcta tcactacttt tttactattt tctatttctc
     6481 caaaagcttt tgctcctaag atactaaaat ctttagcagc tacagcatct aaatctttca
     6541 tgtatgcaat atctgctcca gctacaaatg cctttccttc ccctgtcaat attacaacat
     6601 caatagtttc atcattatta atatctacaa atacttcata aagttcttct aaagtctttg
     6661 aatttattgc attaagggct ttaggtctat tcattttcac tgtacatata tttccatcta
     6721 cttcaacagc tacattctca taaactttaa catcacttgt actcataata ccctccccaa
     6781 gaaattagtt agctaaaact tcaccttttt cttttgcaac acttaactga ctgataagtt
     6841 ctggaagcac tttatgaaca tctccaacta taccaacatc agcatattta aatattggag
     6901 cttctggatt tttatttata gcaactataa actcagcatc ttccatacca gctatatgtt
     6961 gtattgctcc agatatacca catgctatat aaaggtctgg tcttacagtt ttaccagttt
     7021 gaccaacttg tcttgcttta tctaaccaac ctgcatctat agtggcacga gaaccagaaa
     7081 cttctccacc tataatttca gctaattcat aaagtatgtc taagttttct tttccaccca
     7141 ttccacgtcc agcagaaact aatatcttag catcttctat tttaacttgt tttttagctt
     7201 cttttattac ttgtacaact tgaactaatt tatcagcatc attaaattct accttgaaac
     7261 ggttaattac agcttcttta gtttcatcag gttcattttt cttcataacc cctggtctaa
     7321 ctgtagacat ttgaggtctg aaatctttac aaactattgt tgccattata tttccaccaa
     7381 aggcaggtct tgtcattaat aataattttg tatcttcagc tactgcaaga cctgtacagt
     7441 cagcagtaag acctgtatgt attctagcag aaactctagg tgctaaatct ctacctattg
     7501 aagttgcacc aaataataca actatagggt cagctgcttt tattgcttca taagctgctt
     7561 ttgtatatgg ttcagttgta tacactgcta aagcttcatc atctactact attacctcat
     7621 ctgcaccata gtgtgctaat gtatctatta aaccttctac cttactacct aaaagtaatg
     7681 cagaaacttt tgtatcataa tcttttgcta tttctgtagc ctttcctagt aattctaaag
     7741 aaacagtttg aattacattt tctctttgtt ctattactac taaaacgtta cccattcttt
     7801 atcccccctt aaattatatg atatacttct cttttaattt atctatgata attccagctg
     7861 atgttttcgc atcttcattg tatattgtac cagctggttt aactgatttt gtaaatgatt
     7921 taaatacact agttggagaa cctttaagac ctaaattaga agggtcaact tctatatctt
     7981 ttacagtcca tgtttctact acatcatttt cgaaagcatc atatattctt ccaactttca
     8041 tgtatcttgg tgtgttcata tctttaagag ttgttataag gcatggcatt ttaactttta
     8101 agtcatggca acaatcttca aattgtcttt ttactaatac atattcacct tcagttttta
     8161 tttcttcagc atatgttatt gatggaagat ttaaatgttc agctatttga ggtccaactt
     8221 gtgcagtatc tccatctatc gcctgtcttc cagctattat aatatcaaaa tctatatttt
     8281 ttaatgctcc tgctaatgct gatgaagttg cccaagtatc agcacctgca aatgctctat
     8341 ctgttaataa tatacctcta tctgcaccca ttgctaaagc ttcttttaaa gccatatctg
     8401 cttgaggagg tcccattgtt ataacagtta catgagcacc catttcttct tttaatttta
     8461 tagcttcttc taaacctgct ttatcatcag ggtttattat acttggtact ccatctctaa
     8521 ttaaagtacc tgtattagga tctagtttaa cttctgttgt atctggaact tgttttatac
     8581 aaacgactat attcataaat ccttcctcca tatctatttt aatatcgacc tattgtgtat
     8641 attttaatat ttatctattt taataatttt cctgaaataa ccattctttg aacttcacta
     8701 gttccttcat atatttcagt tatcttagca tctctcatca ttctttctac tggatagtca
     8761 cgagtgtatc catatcctcc atgaagttgt acagcttttg tagtaacttc catagctgtt
     8821 tcagctgcaa ataattttgc cattgctgct tctactccat aaggttttcc taagtcttta
     8881 tttatagctg cttgatatac aaggtgtcta gccgcttgta ccttaacttc catatcagct
     8941 aattggaatt gtgtattttg gaattttgat aatggtctac caaattgtac tctttctttt
     9001 acatatttaa cagtttcatc aagagcacct tgtgctaaac ctaaagcttg tgcagctata
     9061 ccaattctac caccatcaag agtagacatt gctatcttaa atccttgacc ttcttttcca
     9121 agtaaatttt ctttaggtat tctgcaatcc tcaaatatta attcactcgt agctgaacct
     9181 cttataccca ttttcttttc tttaactcca aagctaaacc caggagttcc tttttcaact
     9241 ataaatgctg atattccttt gttcccctta gatttatcag tcattgccat tactacatat
     9301 atgtcaccag ctattgcatt tgttataaat atttttgagc cattaagtat gtattcatcc
     9361 ccgtctaaaa cagcagttgt ttgttggcca gacgcatctg taccagcatt aggctcagta
     9421 agaccaaatg ctcctaattt ttctccactt gctaatggtc ttaagaattt ttgtttttgt
     9481 tcttcattac catattgata tataggccat gagccaagag atgtatgagc tgataatata
     9541 actcctgtag taccacaaac tctagacaat tcttcaactg ccattatata tcctacagtg
     9601 tctccacctt ctccaccata ttcttttgga tatggtatac ccatcattcc tgcttttgcc
     9661 attttttcca ctgtttcata aggaaatctt tcttcttcat caagttctgt tgctaaaggt
     9721 ttaacttcat tttcagcgaa gcttacatat agctctttaa gcatctgata ttttttagaa
     9781 tttaaatcca taatagcctc ctcagtattt tataaatatt ttattttcat ttattaatta
     9841 aaatctcggc aacatctata ataaggtatt tgaatatacg tcgagaaatt tataacctta
     9901 atcaaactac gctctatatt tatatgtgat tatactaacc aattatgaca ttaatttaat
     9961 cataaattgt taatttaaaa tcaactatac ttaaaaaaaa tctcgctttt tggaattttg
    10021 tgaaatttca agttagaata agatttcttt cacactggtt agagataaaa taatttgttt
    10081 acgatattta tattaaggta tattaaacat taaatattgt ctgtacatct gttttttagc
    10141 catgtacaga agatttttat cgtataaaaa ttattttaat agagctaaat aatcatattt
    10201 ttatctgact attttttatt tttaaatatc ttacatctat aatagtatca ctcaaaggat
    10261 aatttttcaa taatttctaa ttattttttt aacaatattt attacatttt ttccactttt
    10321 ttgtcatttt tttattgttt cgaaaaaaaa tcagaatttt ttaactttat tttggattat
    10381 tagtttaaat tagattttcc tcttagattt ataaatattt acacaaaaaa actacctctg
    10441 tatagattgt atacagaggt agttttttac taattcctac tatagtttac tatatttttt
    10501 taccaattct tacaaacatc tacccatttt tcacacttag aaaacttctc catttcttca
    10561 catgtaagtt ctattgcaga gttactactg ccacatgcag gaaatactgt atcaaatctt
    10621 ttcattgaat catctaaata gacttttata gaatttttta aaccaaatgg acagactcca
    10681 ccaatagcat gtccagtaaa ctccaagact tcttctggtg tcaacatttt agccttacaa
    10741 ttaaattcag ccttatactt cttattatca atcttagcat caccagctgt cacaattaaa
    10801 attgcatcat ctcctatttt aaatgagagt gtcttagcta ttcttgctgg aataacccca
    10861 gctgcttcag ctgcaagttc cacagtagca ctagattgtt caaattctaa tatactgtct
    10921 tccttgccaa attgtttaaa atactctctt acattactta ttgacattaa atacccctcc
    10981 tagtctgaac ttttataaat ttcatattat tcttaattat atctataatt gatttatttt
    11041 tcaaattatt ccataatatt agcattccat gtatcattat tttttccaac tactagataa
    11101 agtccttttg ccattggaat attgacaata tattctcctt tatactctac actcatacca
    11161 gattcattaa agtacctata atcaaaattt agtccttttg gaattacaaa atcaacataa
    11221 ccttgattat ttggttttat tccatatttc agagttcttt cagttataga atttttttca
    11281 ttaaataaat ctacatttac atatgcagaa cacttattca ttcccttttt attttccaca
    11341 caattttcaa acttcatttt agcactgttt aatttataac tatatttact attattgctt
    11401 attttaaatc ttacaatagt accaacatct ctacttctcc tgctatcaaa atcaaaaaca
    11461 atttcctcta aatgatttat ttcataatct ttgacactta ccatatctct atatactaat
    11521 ttatttatag ctatgaatag tgtaaaaatc cccaaaataa aaaatgaaac ttttataaac
    11581 ttcttcattt ttagtctcct tatattttgt gctattgtgc tataatatta atcaaaattt
    11641 taataattaa acttattctt ttttgaaatc ttaatagaaa aatacattga tattataaaa
    11701 attattattg ttaaaaatgt catactatat aaatttatat acttagtatt agctatatca
    11761 gttacataat ttgaaagata tactcctcca actttactat aattaaaatg ctttacttga
    11821 tatacaatca tactaatcat cttcaaaaga cttccatata caaagtaaat taaaatactt
    11881 actataaaac cacaaacttt catttttcca tatccaaact taaaaattaa aggcagtaaa
    11941 atcgtcaaaa ttgctaaaaa tatatttaca gaaaaaaata catcatttaa aaccatattt
    12001 ctaaagaaca ctagtgatgt caatttactt agtaaggaca caattattgt tacagaaatt
    12061 attaatccta cacctaataa atattttgag taaactatat cttctatact aactggcata
    12121 ctccttataa aaatccttga atcattttca taatcacact caaatgtatt tataaatatc
    12181 aaataactaa caacaatact tactgtataa tatgaaattg aattaaggaa aaaataaaac
    12241 acaaaaaata gcaatatgta ctttaaatta cggataccat ctgaactaat ccctattatt
    12301 aaatcttttc ttattaaatt gaacatcatc tcacctcatt tacataataa tacattagtt
    12361 cttctatagt aggatttttg accttatgct tatcaatttc tatcaatagg ctttttactt
    12421 tcaagctatc tccaacttta acaagtgcct cattataata tttatactct ttcacaccaa
    12481 ctatgctttc tttaattgct tctaactctg ctttatttgc cctaattatc ttatattctt
    12541 gttctattaa ttctttctta acagtaaaaa tctgatttcc tctatttata aaaacaagat
    12601 aatcagcaat tttaattaaa ttatcagtat tatgagtaga aattattaca ctactacttc
    12661 catttttcac aaaatcttgt aagtactgca ttatttcagt tcttacaatt gggtctatcc
    12721 cagcagttgg ttcatccaaa atcaataact tagcattgtg actaagtgca cttgataaca
    12781 taagcttttc agattgacct ttagataaat tttcaatatg catattttct tctatatcaa
    12841 acttattaag gtattcatca aacctttctc tatcaaaatt cttataaaat aaagaaacta
    12901 tttttctaaa ttccttaacc tttaaatgcg gataaaattg taaagaatca tatacaaaac
    12961 caatattgtt tttaaagctt attggagaat tttcaatagt ttcattacaa agttcaattt
    13021 caccttcatc tgcatcaata atccccatta taatttttat taaagtcgtt tttcccgaac
    13081 catttggtcc tataatccca acaatatccc cttcctttaa ctctaagttt atattattta
    13141 acttgaattt tcctaatttt tttgtgatat tccttatgct aaacacatct tcacctctta
    13201 tgcatttttt tatacccacc tatacatatt ttaacacatt cactacaaag gtcatttata
    13261 aatttataaa ttaagaatta taaaactatg ttataataca aagtatgaag aggtgaatat
    13321 atgaaaagaa ttataactat aaatacaaac caattaaata agtcccttga gactaaagaa
    13381 ctcttaacta gaaaacttat caatgctggc tttgaagtgt attcagatat ttatccagat
    13441 acagagctta ttatatcaat tggtggtgat ggctcttttt taagaacagt tagagatttc
    13501 gattttcctg aaattcccat aatgggaata aatacaggtc atctggggtt ttttcctgat
    13561 attttaccag ataagattga tagctttatt gaagcttata caaaaaaaga ttatattatt
    13621 caagaaatgt cacttcttaa tgcggaagta tatacaacta ctagtggttc taatatgctt
    13681 gctgtaaatg aggttgttat aagaggagac aagtctagga ctatacacct aaatttaagc
    13741 cttgataata aacatataca aaattttagt ggagatggta tgattatttc tacttccaca
    13801 ggttcaacag cctataacta ttctgctgga ggtagtattg ttgatataaa tcttgaactt
    13861 atgcagatta cacctttaca tccgataaat accaatgcat ataggtgctt tacatcaagt
    13921 ataatatgtt caaatgaatc tgttataaag attgcaccag aatatagatt tgaagattct
    13981 gtattgattg tcgttgatgg agttgagcac agatttagac aaatcgaaaa tataaaagtt
    14041 agtatatctg atgcaaaaat aaaattactt agaatgtcaa actatgagtt ttggcataga
    14101 gtatctgaaa aatttttata gactgtactt aggaggattt ttaatatgaa aataggaata
    14161 atgagtgata ctcatggtag cttactttac tttgaaaaag cgttaaatgt attatctgat
    14221 tgtgatgttt tattacatgg tggagatgtc ttatatcatg gacctagaaa tgatattcca
    14281 gaaggatata atcctaaaaa atttattgag acattaaata aattagaaaa cattgtgatt
    14341 gtaaaaggga actgtgatgc agatgttgac cagatggtta tagaacatcc tattcagagt
    14401 ccttatgtca tgagccaatt tggtgaaatt agaattatat tgaatcatgg ttatatagaa
    14461 tcagaagaag aaattattga caaagcaaag aaaatgggtg gtgacatatt agttttagga
    14521 catactcatg taaaaaagct atacatggat gataatttaa tagtaataaa tccaggaagt
    14581 acttctatcc caaaagatgg aagccattct gtcgcaatta tagatatcat aaaaactgat
    14641 gatgaggatg agcttgaact tgatataaac ttaattga
//
LOCUS       DAYATN010000099        14552 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.099, whole genome shotgun sequence.
ACCESSION   DAYATN010000099 DAYATN010000000
VERSION     DAYATN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.099"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            32..1660
                     /gene="aspD"
                     /locus_tag="ACGI8A_002690"
     CDS             32..1660
                     /gene="aspD"
                     /locus_tag="ACGI8A_002690"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047688 - aspartate 4-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HFH0180681.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1781..2776
                     /locus_tag="ACGI8A_002691"
     CDS             1781..2776
                     /locus_tag="ACGI8A_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFH0180682.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(2954..3262)
                     /locus_tag="ACGI8A_002692"
     CDS             complement(2954..3262)
                     /locus_tag="ACGI8A_002692"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0180683.1"
                     /translation="MSKDVADEIEKTYAWKHTTTITLLARLTKKGFFISQRIGKHVHY
                     RVLIKEKEYLKIEGKRIFGGLHNNPLSELISKLHDGEEITEEKIIEIGNWIKSWKDED
                     "
     regulatory      3564..3661
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3802..4360
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3802..3831
                     /rpt_unit_seq="aatttacattccatatagttaatataaaac"
     gene            complement(4646..4804)
                     /locus_tag="ACGI8A_002693"
     CDS             complement(4646..4804)
                     /locus_tag="ACGI8A_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180684.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5098..6468)
                     /locus_tag="ACGI8A_002694"
     CDS             complement(5098..6468)
                     /locus_tag="ACGI8A_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HFH0180685.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6684..7499
                     /locus_tag="ACGI8A_002695"
     CDS             6684..7499
                     /locus_tag="ACGI8A_002695"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFH0180686.1"
                     /translation="MNIKSAFIRKRGEKFYVYVEYIEETTGKIKQKSYGSYEKKKDAE
                     KHLIEIKSTINNNKFVAPKDMTFVERCYKYMDMNKNNWSPYTVTNTKSWVKNYIEPLF
                     KNLKLTEVNPSSVQAFVNYNFNNSTFTSAKVRYSFLSSVIREAYRLREIQENSCDFIK
                     LPSKEKYSTNEIYNRKEVLLLIEKLLSNNIEIPILLMLLLGLRMGEATGLRWSDVDLD
                     NNLINVNQILVYSNGKISFKEPKTPKSKRALSAPKELIEKLKIKKLRQSKLKL"
     gene            7529..7801
                     /locus_tag="ACGI8A_002696"
     CDS             7529..7801
                     /locus_tag="ACGI8A_002696"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0180687.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(8164..8244)
                     /locus_tag="ACGI8A_002697"
                     /pseudo
     CDS             complement(8164..8244)
                     /locus_tag="ACGI8A_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8284..8952)
                     /locus_tag="ACGI8A_002698"
     CDS             complement(8284..8952)
                     /locus_tag="ACGI8A_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFH0180688.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9805..12225)
                     /gene="leuS"
                     /locus_tag="ACGI8A_002699"
     CDS             complement(9805..12225)
                     /gene="leuS"
                     /locus_tag="ACGI8A_002699"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFH0180689.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(12579..12929)
                     /gene="rsfS"
                     /locus_tag="ACGI8A_002700"
     CDS             complement(12579..12929)
                     /gene="rsfS"
                     /locus_tag="ACGI8A_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFH0180690.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(13046..13618)
                     /gene="yqeK"
                     /locus_tag="ACGI8A_002701"
     CDS             complement(13046..13618)
                     /gene="yqeK"
                     /locus_tag="ACGI8A_002701"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFH0180691.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(13619..14308)
                     /gene="nadD"
                     /locus_tag="ACGI8A_002702"
     CDS             complement(13619..14308)
                     /gene="nadD"
                     /locus_tag="ACGI8A_002702"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFH0180692.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         4906 a         2376 c         1843 g         5427 t
ORIGIN      
        1 gtgaaactta ttacataagg aggaatttaa catgaaaaat catacttata aagaaatcaa
       61 aaacatatat ggaaaaataa gcccttttga atttaaggac aaattaatag atattgcaaa
      121 atacactgca aaagaaaata acagagaatt acttgatgca ggtcgtggaa atccaaactg
      181 gacttgttca actgctagag aagccttttt tacttttggt catttcgcta ttactgaaac
      241 tcgttctaat tgggatttag gtcatttggc tggaatgcct caaaaaaagg gtattaaaga
      301 aagatttttt aaatttataa atgaaaatat agatatgcct ggagcatatt tagctagaga
      361 tattataaac tttggtataa atgaacttgg atttgatgga gatgaatttg ttcatgaact
      421 agctgatgga attattggtg ataactatcc acttccagat agaatgttac ctcatatgga
      481 aaaaatagta cacgattacc tagttcaaga aatgaaatat gatatatcag gtaaatatgg
      541 tgatgtagaa atttttgctg ttgaaggtgc tacagctgct atgtgctata tatttgattc
      601 attaatggca aatgaacttc taaaaaaagg agataccata gccttaatga ctccaatatt
      661 tacaccttat cttgaaatac ctaaccttcc tcgttacgac tttaaagtgg taaatataaa
      721 tgctaatgaa gttgatgaaa aaggtgctca tacatggcaa tatacaaaaa aagaattaga
      781 gaaacttcgt gacaaatcta ttaaagcttt attcgttgtt aatcctaata atcctgcttc
      841 tatagctatg gatgaaacat cttgtaataa tctaatagat gtaattgaaa attataataa
      901 agatttaatg ataatatcag atgatgtata tggaactttt gtagaagatt tttcatcctt
      961 aatgtcaaaa ctaccttaca acactgttgg agtatattct tattcaaaat attttggagt
     1021 tacaggatgg agacttggta catttgccct tcataagaaa aatgtttttg ataagaaaat
     1081 aaatgattta actggagaat taaaaaaatc agtagataag cgttacagtg atatgagtct
     1141 taacccttct agcctttcat ttatggaaag agttgttgct gatagcagac ttgttgctct
     1201 caaccatact gctggtcttt ctactccaca gcaagtacaa atggcgtttt tctctgcttt
     1261 tgcattgatt gacaaagtag atgcctacaa aaaactcaat atgagtatct gccatagacg
     1321 tcaaaaacta ttatttgaag ctttagagct tccaattaat gaaaacaaaa acaatgctgc
     1381 atattacact caatttgata ttgaagaatg ggctaaatta aactacggag aagacttatt
     1441 taaatttata agtaaaactg catctccagt agatgttctt tataaattag ctaatgacta
     1501 ttcagtagtt ttacttagtg gaaatggatt ctatggtcct gaatggtcaa ttagaatatc
     1561 tctagcaaat ctatatgatg aagcttatac taagatagga aaagccataa gagagatatt
     1621 aaatggttat atcgttgaat ggcaaaaaat taagaaataa ttatattaca ctgaaatatc
     1681 taaattatat gaataaaaga gtttatgtag accttaacta ctttttttga tataatccta
     1741 tcggaattat aaattttatt tatgaaaggc agagaatagt atgagtatta agaaaaaagt
     1801 tgccatagtt tttactggtg gaactatatc tatgacagta gatgataaag tcggagcagc
     1861 tataccaact ttatcaggtg aacaaatact atcaatggtt accaacattg ataaagttgc
     1921 agatgttgaa gtttttaatt ttgatgaaat tccaggacct cacataacac cttatagaat
     1981 gatggaattg aaaaattatg taaatgattt attagcaaga aaagatatta caggtgttgt
     2041 aataacacat ggtacagata gtttagagga aacagcttat tttctagacc ttacaattga
     2101 caacatcaaa cctgtaatag tcacaggtgc tatgagaagt agttctgaac ttggatatga
     2161 tggttcaagt aatttatctg catcagtttg tactgcagta tccaaagatg ctatgggaaa
     2221 aggagtcctt gtagttttaa acaatgaagt attattagct tctgaagcta ctaaaacaaa
     2281 tactctttct ctaaatacat ttaaatctct aactagtggc ccattaggaa taattgactg
     2341 caacgaactt gtccttacaa gagatattgt aaatagaaca attatagata ctgataaggt
     2401 agaatctaaa gttgccttat ttaaagcata tgtaggagaa aatgctgact ttataagatt
     2461 tgctgttgat agtggttata aaggtatagt tattgaggca atgggtagag gtaatattcc
     2521 tcctcaaatg ctttctggag ttgaatatgc tagagaaaaa ggacttcctg tagttatagt
     2581 ttctagatgt cattcaggta gagtattcga tagttatggt tactttggtt ctggtagaga
     2641 tttaaaaaat cttggttgta tatttggtgg ggacttacca ggtcaaaaag ctagaataaa
     2701 acttatctta gcacttggaa aaactgataa ccttgatgaa atcaaagatt tctttgaaaa
     2761 aggaatctat tgttaattct aataacttct atttaattat ttattgatat ttcaacattt
     2821 ttaaatcaaa atctaatact tatgtcagtt aaatgtcagc aaaatatttt atccacaagt
     2881 tttccacaac ttatccccaa tattatccac aagaaaaaag gtagcactta tattagtagc
     2941 taccttttat ttattaatct tcatctttcc agctttttat ccaatttcct atttctatta
     3001 ttttttcttc agttatttct tctccatcat gcaattttga aattaattct gataatggat
     3061 tgttatgtag acctccaaaa attctttttc cctctatttt gagatattct ttctctttta
     3121 ttaatactct atagtgtaca tgtttcccaa ttctctgcga tatgaaaaaa ccttttttag
     3181 ttaatctagc taaaagtgta attgtagttg tatgcttcca agcatatgtt ttttctattt
     3241 catctgcaac atcttttgat attacagttt tataaccagt attctatatg tatttcataa
     3301 tttttaattc tgcatctggt aattttttat tcaacataat atattccttc tttcaattta
     3361 tgtcacttcc tcaaacttat ctagcttaaa ttaataatag gagcttttta tcacaaaaac
     3421 tacaggtaaa tttttctata tattctacgt atttagtctg ttttttaagt gtgaaattac
     3481 gttcatacct ataaatttat tcaattttca aacaatctta ttgaataata actaattcta
     3541 ttgtattatt tagttataga ataaaactaa atcggcaaaa ctagagaaat ctagtgacgc
     3601 aaagctatag agactaagac ttatataaat atcttatgag ttatgtcagc cagttgccaa
     3661 agagatattg ttctttttgt ttttatgaaa gttttttagg gggataataa tttatatatt
     3721 tagagataaa atagataaat gtacacatat attgactgct tatattagca gttcgtatga
     3781 ttattgtaat tttttggata caatttacat tccatatagt taatataaaa caagtgtttc
     3841 gaatttcttt tctgaaacta taccaacaat ttacattcca tatagttaat ataaaacgtt
     3901 atatactgaa agcggtgaaa ctggttacgt agcaatttac attccatata gttaatataa
     3961 aacctgtgcg tgtaggagat ggtaacacaa ctgttgaaga atttacattc catatagtta
     4021 atataaaaca cacatctaca tgtttttcta tctgagttca tctctaattt acattccata
     4081 tagttaatat aaaacttttt aatccttctt ctgaactccc aaagtttata tcatttacat
     4141 tccatatagt taatataaaa cccacgaaat ttaaggtcga tatatcagat ttgaaaaaat
     4201 ttacattcca tatagttaat ataaaacaag ctaaatggag caaccgacca agtcgctaga
     4261 aataatttac attccatata gttaatataa aacttttcta ttaagtgtaa gcagaatgca
     4321 tggaatatga aatttacatt ccatatagtt aatataaaac cccaaaataa attgagtatt
     4381 tccaatgcct acacatatac aaatctctca aatttgcagt gaaccatgag tagtgcaatt
     4441 gataacactt atcacaagtc ctcaatgcct tatatttcaa ctgttaagcc ttaatttctt
     4501 caaaaaatcg aacactgcaa aatttctatg actttattat atcataaaaa tattattttt
     4561 gaatatctgt accaatttgt gatataataa aagcaagaag aactacaatc tattttgctg
     4621 tagagtggag ttcataattt agaaattact tagttttatg gaatctgatt tttaaatcaa
     4681 attcccaacc actcttagtt gccgctttga gtggtttttt acgctttcta aatagtttgc
     4741 tagctatata aactattaag ctagcagata gacttgctag tatgccttgt aaaaaattat
     4801 ccatgtatat tcacctccct tcttaacgtc gggaggataa ttttttgata catgaactcc
     4861 actctataaa ttgtagatta catcttcttg ctaaaaatat tataacatat aattcttaca
     4921 tattttacct atttattact ttttacatta taataatatt cttttaatca aaaaaacacc
     4981 tactattaaa gtaaatgttt ttaagtattt ttaattttaa aatccatata gttcatatga
     5041 aactaaatat aataactcaa catatgagca atatcgagac atttacatac ccactagtta
     5101 tttatattca aaaaaatctt ctccatccgg gttataaaat aatattctac ttgccaatcc
     5161 ttgttcatat ttataatttt ctttatcacc tacataagaa aatgttaatg tcatgcttct
     5221 tgatggatct aatgaaatta tatctaaata aatagtacat ttttctcctg gctttaatac
     5281 accgaaacta ttaaaatcca tttttatttt agcgtattgc cccatatata atgttgaatt
     5341 agcttctcca taaactttta attttgttac atctaaattt tgtgtacttg tattttctat
     5401 atttatagga actgttgggt ctttatattt ttcagtataa tattcaaggt tatcatcata
     5461 acattcaaga taacttcctt tatcttgcca aattatgtga tttgaatcaa aatcaatctt
     5521 taatccacat attatatttt tatttgaaga tggaagacta atattattga tggtattttc
     5581 acactctagc ataattttat cacttagatt tcctatcccg gttacttttg cagcattttt
     5641 aagtatatct ttattacttc caatatcaac tagtacaata ggattatttt tagccattgg
     5701 tgaagcaact aaagcataaa ctaaatcagt accactagct atataaaatt cttttgcgcc
     5761 attatagaac ttatttacta tttttttatt agtatcatat ctatctactc cacccaatcg
     5821 agttgaatta gtatcattta ctaaactatc actcattgat gaagtgccac ctattgcata
     5881 actttctata ccagttgtat taaatggaac actttttcca tcagttaata ctatagctgc
     5941 tttatctcta actgctacag gtgcagcact catagcatct ggctcacctt tgaaagcgtt
     6001 agttaatatt actttattta ctttattaat ggaatttatt tcttttgcta cattataact
     6061 tgtcttaatc ctatcattac cttgaagcct tctagtctct attcctttat cttttagaaa
     6121 agtttctgtt gctttatcaa tggaactttc tcctcctatt atgtatacct ttttagcttt
     6181 ttctactctt ttaagagttg cattaggtat attattcttt ttagtcaaaa ggattggtgc
     6241 atttgttgct cctgcaagtc cactcgcact tagcccatca gccattgtac tatctgcatt
     6301 tataagtatt gcagtagtat aactttgttt gtcagctatg ataccagcag tttcgtattt
     6361 atctgctcct tgaattttat caattttatc aagtgcatta gctgatatag gaaaaccagc
     6421 aacaaacatt gacactaacc ctagtgataa taatttcttt aatttcacaa tatctccccc
     6481 ttttatatat gaaatactat atatcttaat aattctacat taaattttct tttcctcctt
     6541 taacgatatt tatcttcctg ttaatttaag tgtatacaaa tacgtgttat aatgtaactg
     6601 taaagaatta aaaagataat gacataatta gagtagtaca actgttcttt tataaaacta
     6661 acttataaag ggagcgtaca tatatgaata tcaaatcagc ttttataaga aaaagagggg
     6721 aaaaattcta tgtatatgtg gagtacatag aggaaacaac tggcaagata aaacagaaaa
     6781 gttatggaag ttatgaaaag aaaaaggatg ctgaaaaaca tttaattgaa ataaaatcta
     6841 ctataaacaa taataagttt gttgctccaa aagatatgac atttgtagaa agatgttata
     6901 aatacatgga catgaataaa aataattggt ctccatatac tgttacaaat acaaaatctt
     6961 gggttaaaaa ttacatagaa cctcttttta agaatctaaa actcacagaa gtaaatccta
     7021 gttcagtaca agcttttgta aattataact ttaataattc tacctttact agtgctaaag
     7081 taaggtatag ctttctaagt tctgtaataa gggaagcata tagattaaga gaaatacaag
     7141 aaaattcatg tgattttata aaacttcctt ctaaagaaaa atattctaca aatgaaatat
     7201 acaatagaaa agaagtatta ttgctaatag aaaagttact tagtaataat atagaaatac
     7261 ctattctttt aatgttactt ctaggtttaa gaatgggaga agctactggg cttaggtggt
     7321 cagatgttga cttagataac aatctgataa atgttaatca aattcttgtc tattctaatg
     7381 gcaaaatatc ttttaaagag ccaaaaacac caaaatcaaa aagagcttta tcagctccaa
     7441 aagaattaat tgaaaaattg aaaattaaaa aactaagaca aagcaaatta aaattgtaag
     7501 gtatcctgga aaacagaaat aatttagttt gtctaaacac caatttaaat ccgtttctac
     7561 cttcttcact aagaaaaacg tttcacaatt ttacaaaaaa aataatttaa aaaagattag
     7621 aatacatgat ttaagatata caagtgccac tctgctgctt ttagggggaa ctaatatgaa
     7681 agttgtttct gaaagattgg gtcatacaga tataaaaata actatgaata gatattctca
     7741 tgtacttaaa gaaatggaca aagaagcttc cgacaatcta agcgaacttc tatttaaata
     7801 gtaaaaactg actgtcagtt aaatgtcagt taaacctaaa aaaataggtt ttgtcagttg
     7861 attatcagtc aattatttta taatagtttc acttaaaaac gtctatatct acacatttta
     7921 ctatttgttt atgcttatta atatctatct gttattatta attctaatag cttctattta
     7981 attatttagt tcagtttaca ggaaactaca cacaaaccat aaaataggat gttgacaata
     8041 taacagatag tatgctcctg taaaaggtaa cgtactatct gttatgttgt ctggatttcc
     8101 aaatggttac cctttggcac acaactttgc tatctccttg gtaaactaaa agtttgtaat
     8161 ctaaaatcct aatccatctc tataaaattt aattgacttc tccatacttt ttacacctaa
     8221 acatataaaa gtaattttat tcacttaatg acctccataa ttcccaaatt agctatttgg
     8281 aacttaacta aaaaatccta atataagggc aactattata atggcaatcg gaatacatac
     8341 tgtgtaatcc tgttccatag agtcatcatc tgaatgtagt tttacaattc gcaatttagc
     8401 aatcagtttg aagcagaaat agcctattat tgttccaatt acattcgtga gtaagtcgtc
     8461 tatatcggtt gctctgtgta acgtaaaaag ctgaatagtt tcgataaaaa gagataatcc
     8521 aaaaccaatc aagaatgtat ttctcaaacg ttcaaaggtt ttgctgataa gcggacataa
     8581 aaaacctaat gggacaaaaa gtaaaatatt caaaataaaa ctcaaactaa atccatcact
     8641 aaaaggaata agatttatgt ttggattaaa gaacgtttcc cctaactgtg acagtcttat
     8701 atattcactg aatgtaggga taccaacaat atgcgatagt gttaaacata agtagtaata
     8761 cattgccagt gataaaatag atgtttttac ctttggaata tcactatttt tcttgaatat
     8821 tacaactaag aaaataccaa tcaagataat tcccaacaag aaaataggat taattaacaa
     8881 taaatctttg atagcttcaa acagactgag ttgaatttct acacctgatt gattatttgt
     8941 tacagtctcc atttgtacat acctcctcat aacatttcaa ttatttctaa aacacaaaat
     9001 agaagataaa tcttaaaatc atcctatcaa aatttttaaa tttgccttta tcagtttata
     9061 ccaccagtta attcctgtct tacttctgta tggtattctt gggattcttt catcaaatta
     9121 gacaaaaaat ggttaccata tcaataaact ggttacgctc catatctccc gaagcaacca
     9181 tcccctgaga ataaatccgt atcaggaatc tccacatcta ctgcacctat cacaagttat
     9241 ttgataacat cctcagacaa tcccaacttg gatatatcga caacacccat tagaatacca
     9301 ctgctgacca tacttgtttt cctgctgata attttaatca actgggaagt tgaaacgcta
     9361 tcttttttac aagttccttt ttagaaacat ggtagctatt atatgaatct gaatttcgtt
     9421 gtctgggtgt ttcatataaa gcatagatac gcaccttttt tgcctgtttt cattatatag
     9481 aaaaaaattt caaaatacaa taattatatt tttttcgttt aagcttagag cagttccttg
     9541 atgaacaaca ctttggaaac tgaatgattt acataccact taatacatta actcgattta
     9601 acattaatta taataatatt taaaacaaca actagggtat ataagaatga actttttagt
     9661 tctttaatca tttttttgta taaaatcaaa tctattttta taatttcaac aattttataa
     9721 tttcaacaat gaaaaaaggc ttatctctat tttgagacag cctcgtttat tttataaatt
     9781 tatatttttg ttcatatttt tgagttattt aacaactata tttactagtt ttttaggtac
     9841 agctacaact ttaactatag ttttaccttc aactaatcct tttatttttt catcttctaa
     9901 agctaccttt tccatctcat ctttagatat atttgagtta acagttaatt ttcctctaac
     9961 ttttccatta acttgtacaa caacttctat ttcatctttt actaaagctt tttcgtcata
    10021 tttaggccag tctatgtcaa atacacttcc ttctttacct atcatagtcc atagttcttc
    10081 acctaaatga ggggcaaatg gtgcaattat agttactata gtttgtacac cttcttttat
    10141 aactgcttcg tttatattat ctaactcttt atatttatac atatcattta ttaattccat
    10201 aagagctgat atagcagtat taaatccgaa tttttcactt aaatcagcag taactttttt
    10261 aagtgtagag tgtatagtat atctcatatc cttatcttgg gaatttaatt ctccaaactc
    10321 tacatccttt ttaaccacat ctgctaactc gtcaaccaat ctataaaccc tatttaagaa
    10381 tctaaagcat ccatcaacac cttgctctga ccagtccaaa tctctttctg gtggtgctgc
    10441 aaataatacg aaaagtcttg cagtatctgc tccatactca tctattatat ccataggaga
    10501 tactgtattc cctttagact tactcatttt agaaccatcc ataagtacca ttccttgagt
    10561 aagtaagttt ttaaatggct cattgaagtc taccattccc atactcttaa atgcctttac
    10621 aaaccatctt gcataaagta aatgcattat agcatgttct actccaccta tatattggtc
    10681 tactggatgc catctattaa ctaattcctt actaaatggt tcattttcat tattgctatc
    10741 cacatatctt aagaaatacc aagaagaatc aacgaatgta tccatagtat caacttccct
    10801 tctagctggt tttccacagt gaggacaagt tgttgacata aattgctttg aagtcgtaag
    10861 tggagattcc ccttttccag taaattcaac atctgttggt aaaagaactg gtaaatcttc
    10921 ctttttaact ggaactattc cacattcatc acaataaacc acaggtatag gacaacccca
    10981 gtatctttgt cttgaaacta accagtctct aagtctataa ttaattgttc tctttcctct
    11041 accttctttt tctaacttat cgattattcc ttcaaatcct tctgaagcat ctaatccatt
    11101 aaattcttct gaatttatca ttttgttatc ttctgttatt acatcaatta tgtctaaatt
    11161 atatttttca gcaaaatctc tatctctttc atcatgtgct ggaactgcca ttatagcacc
    11221 tgttccatac tcaactaata catagttagc tatccataga ggaactttct tgccatttaa
    11281 aggatttata acatatctac ctataaacat tccttctttt tctacatcag ctgctgttct
    11341 ttctatttct gtcatagtat gcattttttg aacaaaagct tcaacatctg cttcatattc
    11401 agtccctgca accaattctt taactaattc atgctctgga gcaagtacca tataagtaac
    11461 tccataagtt gtgtctggtc tagttgtaaa tactgtcata gatttatcag ttccatctat
    11521 atcaaaaact aaatctgctc ctgtactttt acctatccaa tttttttgca ttgttttaac
    11581 tttttctggc catccatcta atatatctaa gtcattaagt aattcttctg caaattcagt
    11641 agtcttaaaa taccattgtt ctaaatcttt ctttaatact gttgctttac atctctcaca
    11701 tgctccttga acaacttgct catttgcaag aacagtctca caagaaggac accagttaac
    11761 atatgatttt ttcttatatg caagcccatg ctctaaaaac ttcaaaaata tttcttgtgt
    11821 aaatctatag tattctggcg ttgaagttgc aacttcttta tcccaatcat aactaatacc
    11881 taataaattt aattgctctt tcatttcttc tatattttcc attgtccatt tatgtggatg
    11941 tattccatgt tttatagctg cattttcagc tggtaatcca aaagaatccc atcccattgg
    12001 atgaagtaca ttatatcctt ccattttttt gaatcttgcg acaacatcac ctatagaata
    12061 atttctaaca tgtcccatgt gtatttttcc agatggataa gggaacattt ctaatgtgta
    12121 ataattaggt ttttcagttt gtgcagtatc cattttatac tgattattat ctttccagat
    12181 tttttgccat ttcgattcta cttctttaaa attatatacg ctcatatttt gcctcctatt
    12241 tttaattgtg tataactacc ttatttagta ttatttactt ataatttcca aataattctt
    12301 tgaaaataaa aaatcctcgt cactaaactt acgtttaggg acgaggataa atcttcgcgg
    12361 taccacccta attagcctac tacatatata gactctctta gatagctttt aatccagcca
    12421 tggagaatag attttaatct agtaagttca aagttatata atattagttc tcaccttcca
    12481 ctaactctct gaaaaaatat aatcttttac tgctctagtc tattcaattt aatataattt
    12541 ttgaaatttt ctaattataa taacatatat tatcatgttt atgctaatgt gtcaatatct
    12601 atataaggtg catctttcca taatttttct aaattataaa accctctatt ttcctcatta
    12661 aatacatgaa ccattatgtc tccataatca aggagtaccc aagtttgagt tccttgtcct
    12721 tctttacctc ttggctctaa tcctagtttt gctaattcat cttccacatt gtcagcaata
    12781 gcttttacct gtctctgaga agatgctgta gtaataataa aataatcaca caaactagaa
    12841 acttttccta tatttattat aactgtatct tgacccagtt tatcttctat ggcatcatat
    12901 gctatttttg tcatttgttc cactgtcata taaaaattcc tcactttcct aattaaaata
    12961 cacttagtta ttgtaacata ttaatacgat tttgtaacat agtcaaattt aaatttatta
    13021 atatgtttgc ctttatatag cttttttata ataacttttc tttaattaga tagtttcttg
    13081 ccataactgt tcttggatgt atctcttctt ttttattgat gacaaacatt aaagtattat
    13141 taaaagaagt taaaagtgct tcatcaagct tccctccata tgctaattct cttaatgtat
    13201 ctacaactgg gaattttctt ccttcttcaa ttaaatcagc tatgtaaatt attttttcta
    13261 aaagagacat gtcctctctc cctgttgtat gatatttgat tgcatttata atatcttcat
    13321 cttccacttc aaattcatac tcgcatatgt acgccccaat tacactatga gacaaggaac
    13381 gatttccatc ctctattggg tctaaatata tttcatactt atttacataa tcctctattt
    13441 ccttgtcact taaatacttt gcacaatcat gaatcattcc tgctaagtaa gctttttctt
    13501 tgtcacatcc ataaatctca cataattttt cagcacattt tgcaacattt tttgaatggc
    13561 taagcctccc cactggtagc atttgattta gtctttggtt aatattttct aagttcattt
    13621 aatcatctcc atattgatat agtttatttt catataaata attttctaca ttttcgggta
    13681 ctaagtaacg tattgacttg ccttcattta gctgttccct tatatatgtg gacgatatat
    13741 ccagtgatgg aacataaact gttataatgt cagcattata cttcttagtt agtttttcta
    13801 tagtctcttg tgaccttaac aaactaattc ctggtctagt tgcggctata aaagtagcta
    13861 actcaaagtt tttctttaca tctttccact cttcaacact acaaattgca tctgcacctg
    13921 ttataaaata aatgtctgca tttttgtatt ttttctttaa gtattttaat gtatctattg
    13981 tataagtcct tttctttctt tcgatttcta cttttgaaac taaaaaatct tcgttgcttt
    14041 cagttgctag aagtgtcata ttatatctat catacttatc tgtagtgata cataatttat
    14101 gtggtggatt tcctgatggt ataaagatta ttttgtcaat gtcatacttg tctctaataa
    14161 attctgctgt tgctaaatga gcatagtgta tagggtcaaa agtacctccc aatataccta
    14221 ttttgatttt gcctttatgg cgattaaatt tctctagttt ttcagtattt ttcaactctg
    14281 ccatcttaac tagattttca cttctcatat tttgtctcct tttaatgtta taatactatt
    14341 gtaccattat tttttatttc ttaaagctta taatttgttt ttttgggtat ataattatat
    14401 ttatatgcta aaaaacactt taaaaaataa ttatttaaca taattctgct ttacttttta
    14461 ataaaaataa ctttttacta ttttgtctta attttgataa aatataacat acaaatataa
    14521 gttttttgtt aaattttatt agttgctttt gt
//
LOCUS       DAYATN010000100        14524 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.100, whole genome shotgun sequence.
ACCESSION   DAYATN010000100 DAYATN010000000
VERSION     DAYATN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.100"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            234..3332
                     /locus_tag="ACGI8A_002703"
     CDS             234..3332
                     /locus_tag="ACGI8A_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0180693.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVTLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3455..4741
                     /locus_tag="ACGI8A_002704"
     CDS             3455..4741
                     /locus_tag="ACGI8A_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFH0180694.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(5120..6226)
                     /locus_tag="ACGI8A_002705"
     CDS             complement(5120..6226)
                     /locus_tag="ACGI8A_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFH0180695.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(6261..7802)
                     /locus_tag="ACGI8A_002706"
     CDS             complement(6261..7802)
                     /locus_tag="ACGI8A_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFH0180696.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(8164..8910)
                     /locus_tag="ACGI8A_002707"
     CDS             complement(8164..8910)
                     /locus_tag="ACGI8A_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0180697.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(9122..10432)
                     /locus_tag="ACGI8A_002708"
     CDS             complement(9122..10432)
                     /locus_tag="ACGI8A_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFH0180698.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(10445..10915)
                     /locus_tag="ACGI8A_002709"
     CDS             complement(10445..10915)
                     /locus_tag="ACGI8A_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180699.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(10919..11770)
                     /locus_tag="ACGI8A_002710"
     CDS             complement(10919..11770)
                     /locus_tag="ACGI8A_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HFH0180700.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            12694..13605
                     /locus_tag="ACGI8A_002711"
     CDS             12694..13605
                     /locus_tag="ACGI8A_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0180701.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            13780..14277
                     /locus_tag="ACGI8A_002712"
     CDS             13780..14277
                     /locus_tag="ACGI8A_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0180702.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
BASE COUNT         5530 a         1828 c         2236 g         4930 t
ORIGIN      
        1 gaattaatta taaacacata atgagataat taattataaa tatattaaat taggtattac
       61 aaaactctat tgtacgattg acacaataat aattctattt actaaaaaat agaacctcat
      121 taaggtacta ttttaaaaca taaagaaata taatatcatt gccaaatata tagcaaaatt
      181 ttaaaagctt taaaaattca atctggaaaa tgaataaaag gagatatata taaatggatg
      241 taataaattt aatagatgag ttggatgaaa ttgtatatgt ttcttcttta gatacatatg
      301 aacttttata tattaatgaa attgggaaaa aggcaatggg acttaatgat atatcccatt
      361 taaaatgtta taaagcatta caggggaaag attctccctg cgaattttgt acaaactcta
      421 ttttaaaaga agatgaatat tacatatggg aaaatacgaa tactcgaaca aatagacatt
      481 ttatattaaa ggacaagtta attaaatggg agggcaaaca tgcacgatta gaaatagctc
      541 ttgatattac agaaaaggaa gatataagta agtcaatagc agaaaaatta gagataaagg
      601 agttattagt tggatgtatt aaacatctta taacagaatc taatttttct gatgctgtaa
      661 atcttgtatt aaccaatatt ggtaaatttc ataaagctga tagagcatat gtttttgaag
      721 tatcagaaga tagaaaaaaa gtaactaata catatgaatg gtgcaatgaa ggtgttgatg
      781 aacaaaaaca ggcactaaca aatcttgata ttaatgaatt aaagaattgg tttttattat
      841 tcgaacaaaa aggttttgta ataattgatg atatagaaaa tataaaagaa gaacagccaa
      901 tagaatatga aattttaaaa cctcaaaaaa ttgaaagttt aatgacatct tcactaaaac
      961 aagatgataa agtagtaggg tttattggag ttgataatcc acataaagct gttggagata
     1021 cctctctttt gacatcccta tcttattttt tattaactga gatgaaaaaa agaaaagtag
     1081 aaaataaatt aaattatatg agctactatg acttcttgac tggtgcatat aatagaaata
     1141 aatatattaa gtatatagat aattttcaaa aaggaaaagt taaatccata ggagttgtat
     1201 ttgtagatat aaatgggtta aaagatatta atgataactt tggacatgct aatggcgatt
     1261 ctgctattgt gtcagcatgt gtaataataa aaaaatactt tcattcttat aatgtttacc
     1321 gtattggagg agatgagttt gttgtattat gtgaagatat atcaaaacaa ctatttgaag
     1381 aattagtgag ttggttaagt gaagaattta aggatgagat gatatatagt gtagcagtgg
     1441 gcagtatttg gagtgacgat aatattgata tacataagct aattaaagtt gctgatgaga
     1501 gaatgtacca agataagaag aggtattata ataagaacaa acttagaaat aatgaaatga
     1561 aagtaattag tgacttagct gattcgacga ataatatttt atttaaacat aaagagttag
     1621 ttgataaggt ttattttgat gatttaatga taaaagggta ttctaaggct tatgaaaaat
     1681 tagcttataa tacatatgat ataattttat ctatttgcat tgatacgaat ttaattactc
     1741 caatttatca atcagagagc aatattttta gttttcctat aaaggaaaat cttgaaaaaa
     1801 ctttttcata cataaaaaaa catttaattc atcctgatga tataatatta tttaatgata
     1861 accaccttct aaaatctctt gatagtctta ataatagcaa gcaagacgat ttagaactaa
     1921 attttgagta tagacgttta ggaatagata aaaagtacta ttgggcatta atttatatgt
     1981 taaagattag tagtaaaaat aagttaggtg agaaacaaac acatatttta gttgctataa
     2041 aaaatataga ttatcttatg caactacata aaaaccaaaa agatacatta acaggactat
     2101 acaattatga aagtttttgt gaacaaataa gtttaatgct taaaagatat cctgacaagc
     2161 gttatgcaat tataatgatg gatatagata agttcaaggt aattaatgac atttatggag
     2221 ttagaggagg agataaagct ttaatatatg ttgcagagtt gattcaaaaa aacattggtt
     2281 ctaatggtat atgttgtcgt atgtatgcag atgtattttg cattttttat gagagttact
     2341 ctgatagaga tattcataag gtaatttata agattaaaaa tagcttaaat agaaaaaaat
     2401 ttgaatatat gttagtacca tgttttgggg tttatagaat tttggataat catattccaa
     2461 ttacaaatgc gtgtgaaatg gcaggatatg ctcacaaaaa agtaaagaaa caaagtatca
     2521 ataactatat attttatgat gatactataa gaaatgatgc acttgaagaa aaacagatgg
     2581 aaagggaaat ggaatacgca cttgaaaatg ggcaattcca tgtatattta caacctaagt
     2641 ataatcttaa tgcaaataaa attgttggtg cagaagcatt ggttagatgg gttcatccag
     2701 aaaaaggtat aatacctcca attaaattta tttcactatt tgagaagaat gggtttatta
     2761 tcagattaga tatgtatgta tgggaacaag tatgttcttt gattagaaaa tggatagatt
     2821 taggtgaaga gccagttcct atctctgtaa atgtatcaag attacatttg cataatccac
     2881 attttaaaga aattgtttta agtttaataa ataaatatag tataccaaaa tcttttttag
     2941 aattagagct tacagaaagt ttatttatta aaaatataaa attatttact aagataatag
     3001 ccgaccttag aatggatggt attgttttaa atatggatga ctttggttca gcatattctt
     3061 cattaaatat gttaaaaaat attaatattg atacattaaa gatagattgt ggtttcttta
     3121 atgaggaagg aatcacagac agagaaaaaa tagttttaaa gcacactata gccatggcaa
     3181 aagatttaga tatggatgta gtggcagaag gtgtagagac aaaagaacaa gctgaatttt
     3241 tagtgacttt agattgtgtg gtaattcaag gttattatta ttgtaagcct attccaagtg
     3301 ctgattttga gaagttactt tatcaaaaat aagctaatta ttccattcta aatgtaggta
     3361 ggatggagta gttcaaaata attaaataga agtagaaaat agtgaggata taatttttaa
     3421 aaagtataat atttatggat taggagcgaa gtttatgata gtgggtatta taggtccttc
     3481 agactctggg tttaagataa aggatgattt aaaacagata gacagtgatt taaagactaa
     3541 aatatatgta agagaaaaag tagtagatac aatagaagtt atttctaaat gtgaagacga
     3601 atgtgatgct attatattta caggatgtgc agtttatgaa tttataaaat ctaagtatga
     3661 gataagtaag ccacattcat ttgtaccgag aagtgggacg agtataatga aggcattttg
     3721 ggcaataaaa agtgcaaata taaaattaga taaattcagt atagatgtag ttgatagata
     3781 tgttgtggaa gatgcactta aagaatttga tattaaagca acgagtgtat tttgtaatcc
     3841 attttctttg gaagtaggag agtctgaact tgtagaatgg catataaaat tatttgaaga
     3901 aaataagacg gatataatgt tgactggttt tggtgcagta tataatgaat taaaagaaag
     3961 aggctatcct gtatttcgtt tacaagctac tatacaatta atcaaagaaa gttatgacaa
     4021 ggtaaagagt gaatatgctc taagtaaggc aagattttcc caaattgctg ttgaaatatt
     4081 gagtttaata gattataaag agaagataga taactattat tctgacatga taaaaaaatc
     4141 agatatggat aagcttgtag taaactatgt aagaagcatt caaggttcct tatttttact
     4201 tggaagaaat gattatgttg tatttgttca taaaggggta gctgataatg agtataatta
     4261 tgataagcta ttcaatctaa agagagagat aaaagacatg ggcttttctc ttagtatagg
     4321 aattggtact ggagtaactg cgtatcaggc tgaaaataat gcacataaag cattaagaca
     4381 ctccatagat tcaaaagaaa ttggtatata tttggtagat gaagatgaaa acataagagg
     4441 acctttagct tccgaaaatg aattaaatta ttctttgatg ctatctgatg aaaaggtaat
     4501 cgaaatatct aaaaagactg gaatgagttg tgagtcagtt gcaaaaatta ttgctataaa
     4561 tgagaataga aaaagtaaga tttatgattc aaaagagtta gcagagtatc taaatgtaag
     4621 tgaaagaagt gcaagacgta tattaaataa aatagtaaat gcaggtttag gtcgaatatg
     4681 tgcaaaagag actagtattg gtggaggaag accaaaaaat attactgaaa tactgtttta
     4741 agatatttta taaaactgta taaatcatta tattgtataa aattctacaa caaattacta
     4801 tataaagtat taaagcttat tatgaaaaaa attagaacaa ataattatgt aaaatactag
     4861 aataaattat taatataaaa tactagagcc tattattaaa aatgctagaa taaattatta
     4921 tgcaaaatat tgaagcttat tatgaaaaat gttagaacaa attactatat aaaatatcaa
     4981 agcttattat gaaaaatgtt aaaacaaatt attatataaa aatactaaaa cctatgtaaa
     5041 acaactctaa tatataactt ataaagttga ggatacatag gtttttattt ttataattta
     5101 aagagttcta ttatgatatt tataatttaa tcatatgata aactattttg gcagtatcat
     5161 ataattcgtc tatatttaca tattcattaa ctgtgtgtac atcatacata cctagactta
     5221 atatagcaca attatatcca agattagcta atatatttgc gtcacttcca ccaccaatta
     5281 ccattggatt tggaacaaca ccaacttgtc ttattgcttc ttcacttaac ttgaaaacat
     5341 gactatctct actaagttca aaactagggt aagacatatt atgttcaaaa gtataagtag
     5401 tattaaattt agatgcagcg tccttacaac atttttccat atgattaagc tcatactcta
     5461 aagtttcagg tatatgagac ctaatttcag cagtcaatgt aaccttatca gttactatgt
     5521 ttgtagctcc accaccttct atttcaccta tatttgaagt agtcaatgaa tctattctgc
     5581 ctatgtgcat gttgcttatt gcatgagagg ctactaatat agcatttaac cctttttcag
     5641 gttctatacc agcatgtgct ttctttcctt gaaaagagat ttttatagat tgttgagcag
     5701 gagctttgta tgctatcgaa ccaatagctc caccagaatc taaaatcacc atatctttac
     5761 aaggcaagtt gtcaggatta aagtttttga caccatgcat accagcttcc tcacagattg
     5821 taaaacaaag ataaatatct ctatgtggta ttttttctgt aataacatgt tctaaagctt
     5881 caagtatagc agctattcca gctttatcat ctgcaccaag tgtagtagtt ccatcacttc
     5941 taactacatt accatctagt attggtttta cacctaaaca aggttgaact tggtccatgt
     6001 gtgcacataa acaaagtggt ctacttcctt cttcaccttt tatataggca atgacattac
     6061 ctgtattacc accatatttt tcacctgcat catctataat ggcatctata tttctttctt
     6121 ttaagtagtt taccaaccac ttagacattt ctaattcttg attagatggg ctatcaattc
     6181 taaccatatc cataaaatta tttataagtc tatttttatc taacataaat tatttctcct
     6241 tttagaaaga tttaaataaa ttacatgcta tatgaaggaa gtattccagg tcctagtgga
     6301 atattaaata ccataaacaa tattaatagt atagtccaca ctattaagaa tgatatagag
     6361 tatggtatca tattagcaat tacagtaccc ataccagcat ctttatcgta ttttcttgtg
     6421 aatgctagta taatagggaa gtacggaaat aatgggctaa gtggatttgt tatacaatca
     6481 cctatacgat aagccatctg agtaagtgct ggattgtatc caaccatcat aaacattggt
     6541 acaaatatag gtgcaagaat agcccacttt gcagatgcac tacctataaa taaatttata
     6601 aaacatgata atataataaa tcctatcaac aataaaggtc catttattcc tgctgatttt
     6661 aaaaattctc cgccctttac agctagtatc agacctaaat tactatttgt gaataattgc
     6721 aaaaattgtg aagcagcaaa tgctagtatt atatatccac ccatatcact cattgcacta
     6781 gccattatag agactacatc cttatccttc tttattttac ctatagtttt tccgtataca
     6841 acaccaggta tgaaaaatgc tagtgctatt ataggtacca tacctttcat aagaggagca
     6901 ttgtttgata gtaaatctcc tgtttctggg tcagctaaga atggtttttt accgattaca
     6961 ctaagagcta ctataatagc cacatatatt aatatactta tactagcttt tttaagccct
     7021 ttcttttcta catcactaag acttccatca acatctagtt ctgcatttcc ttcatattta
     7081 ccaaatctag gagctacaat tttttctgtt acccatgttg ccaaaagtat tagaacaaaa
     7141 gttgatgcta tcataaaaaa taagttcatt gttgcattgg cttgaaatcc agaatcaatc
     7201 atttgagctg ctggaactgt aaaacttgca gcaagtatat cattgacact tatcataatg
     7261 tttgcggcaa atcctagaca gactgctgca tatccagaaa ataaacctat aagtggatgt
     7321 ctaccaacac caagatatac aagtgcagct aatggtggaa ataatattgt acctgcatct
     7381 gaagctatat tggcaagcat acctatagtc attacaacaa gagttactct tgtttcagga
     7441 atcttgctta ctgatgcttt gattgttgct gtcataagtc ctgttttgtc acatacacca
     7501 gcacctatca tagttactag aactagaccc aaaggagcaa acccttggaa attgctgact
     7561 atgtttccta aaagaatttg aagctgttct acagtcaata aatttacagc ggttatagtt
     7621 tctccagtac caggatgaac tactgatata cccatagaac caactagact agatagtata
     7681 agtaaaagta cacataaccc taaaaatatt gtaactgggt ctggtagttt attaccaaca
     7741 gtttctattc tgtttaatgt cctgtcaaaa aagtttttct tcttaactac gttatcattc
     7801 ataatatcct ccctaatgtt taaaaatata attaaaaaat aagtattgtg gttaatctta
     7861 caatttaaat tctgtttgtc gatttaagac gtgtccacaa tatgtcctaa aaaaagtata
     7921 aaataaactt tttcctgtgt caatgcagtt ttctgaattt tctggttgag tggtgttagt
     7981 taaatatgct ttaagtagat taatgacgaa ttttatctaa tgtatttaaa taacatatgc
     8041 actttaaaat taattttaaa acaaaatttt atgaaaagct agtggaagat taagttatta
     8101 aaataagtag cgtagtaaag tgttagattt tttaattaat caaataaatt tactttgtat
     8161 tttctacaaa aatctgtgaa tatcttttga attatgtaga ggatataaat ttattttatt
     8221 tacatagatt agattttgtt ctaatttact gtattcaaat ggttttccat gtccaggata
     8281 aatcatttta ggttttgtat tgatgattgt tttccaagat tgaataaatt ctagtttgct
     8341 ttctgcaaat atggtaattt tgtgagtact tggaaatcca ttcattgcag aatctccaca
     8401 aaataaaaca ccttcatcaa ttagtagtga aattgaatca tcagtatgtc ctggtgtatc
     8461 tataattttt ccatttagtt ctttttcaat ttctttacga ttcttgtcag acacaagaat
     8521 acaccttttt tcaaattctt gtttaagtgg agaaaaacta tgctcacctt ttccaaatag
     8581 tttcataatt tggcaaaaga gaaaggcagt acggctggta caaccaccaa taaaagagtt
     8641 ttgaccttta tatagaattt ctaaagcttt atcatgcata acaatctttg tattaggaga
     8701 ttcgtctaga atctgattta gaaaaccaac atggtcatca tgagcatgag ttagaaatat
     8761 gtatcgaata tctttgattt ttatgttgaa tttagaaatt cgttttttga aatgtttaaa
     8821 accattttca tatccagtat caatcaagac aaagccatct tgaatagggt acaaccatgt
     8881 atttataatt cgatttcctg cattaaacat tttttcacca ccttgaatta gaatagaaat
     8941 ttatattaat tagaaagaat atagcatagt tctactgata tttcaacgta gctctaaaag
     9001 ttttaaggag gataatatat tttagtttaa tctaaaaact aaaagaaaag aagtatactg
     9061 ttacaaaaac aaagagtggt ataactttta cagtagatac caccctatta taaaaataga
     9121 cttataaagt tttatcgtgt ttttctaaga atgttagata ttcttcttta gtcataactg
     9181 gagtaaaggt atctaatata tctttagcat ccttagcatt atcaaaaaga aggtctacaa
     9241 ctgtaagtgc cattgcttta gcaggaatta tgtaagctaa atcttcatca actatttcaa
     9301 aatctcttgt atgaagagct ccttttacac caccaatcat agggtgaaga gtaggcatta
     9361 tatgagacac atctccaaaa tcaaaagagc ctgtaaaatc tccaccatca ataacttttc
     9421 cttttagacc taaatcttca agattgtttt taaataaatt atcaagactt ttgtatcgta
     9481 atattggaag atatccagga atttcagtta tctcaacatc agctccaact gccattccac
     9541 cagccatcaa agccttattt attctttcat tagcatcaat cataccattt atagttctag
     9601 ctctaacata tgattccata tgaacatcag caggaacaac atttactata tctccacctt
     9661 ttgtgatgat agggtggaat ctaactcttt cagactcttt aaaagtttct ctaagtgcat
     9721 tcacgttgtt tatagcaagc attgctgcat ttaaagcatt tattccatca tgtggagcag
     9781 aaccagcatg agattcttta cctataaatt taaccttttt tccaacaaat ccattactct
     9841 caggaccaac tagagcctta tcttctccta aatcagaagc atgaaacatc atagatatgt
     9901 ctatatcatc aaaataacct ctttttatta attcttgttt tccaccaaaa taagttattt
     9961 ttccttcttt ttttaattgc tctctgtatt ccaactcaat aaactcttca gcaggagtag
    10021 ccataaaaga tactttacca tctaaatgtt ctaaaacgcc actttttact aaaccaacag
    10081 cagcacctaa cattccagct atttgaatat tatgtccaca agtatgagaa gcacctattt
    10141 cattagcatc tttatgttct ttacatgata taccatccaa ttctccaagt atagatatat
    10201 gaggaccttt cttgctagaa tttgcatctg ctttgcatcc tgtaactgct atattttttt
    10261 ctacagataa atttaattct tgctcaaaaa agtttgaaac agcttctgta gttttaaatt
    10321 ctttatatcc atattctgga tttttgtata ttccttgacc tatttttaat attttttctc
    10381 tattttcatc tatagttttt attactagag attttaattg ttctttattc ataataaatt
    10441 cctcctatat tttaccttga atacttaaaa ctatatgagc tatactcgca catgcaaaga
    10501 atatagttgt aaatacaata agtgaaatta taactatttt ccatgacatt tcttttaaag
    10561 cttctatttt gtttccaaca gaaattcctg caaaagctag tagtggagta gttatagcca
    10621 taaagttaat attattagtg ttagttaaga atacttcaga tgttggacta tatggcatac
    10681 taagtaaaag gccgattata gttgcaaatc caaaagctgg aaagattgat ttaggaaata
    10741 aatcttttat aatcatagct gccatagcag ctaatattag catcaccata ccaggaagag
    10801 ctgtaactat agatgtacca acgctaattc tttgtcctat aagaattata actccaataa
    10861 cagacataac caataattga tatgaacttt tctttaaatt taaattagca aacatttatt
    10921 agcactctcc ttctgtagat ttaatttctt tagttttttt atttctcgaa aatttaggtt
    10981 ctaaaatctt atacataaaa ttagtaaatg gtaaggatac aaaaactaag aatataattc
    11041 ctgttatact agacatcata ttgcttgttg cagcataggc aagtattgta tctgccattt
    11101 caggtggtac catggtagat aatgaaccag cagctgctgt catcatactt ccactaccaa
    11161 caccacaggc cattcccaaa gcatatgggt gaagaccact ataaatagat attgaaccta
    11221 gtacactaaa gtatatagta ccaagaactg ttcccattaa atatgtacca agtacaccac
    11281 taccttctgg tgagtttatc ccatattttt ctgatataac tcctaaagaa ggttcacgac
    11341 ttatacttgc agtagctcca acagcttctc ttttcattcc aaacattaat gcaagaggta
    11401 atgcaacgat tggtgcaagt atatgaccaa attcttgagc gataaacgct ggtccagctt
    11461 gaattatttt gtcaatgttt ggtccaactg tagtaccata ttttatacca agcattacta
    11521 gtgcaattcc aacaacttca ccagcaatat ttatttcttt ttctccaacc acttttttaa
    11581 gtgctttaat ttttttacct aataaatctg gtgttattat aagtccaatt ataactgcat
    11641 aaagcatagg gaacagtact atagaggctt ttccaatagc tatagttctt tgccctataa
    11701 attcacatat tactaccaag attaagcaag ttaaaaatac ttgaataaaa tgttttttat
    11761 taatttccat gatttcctcc ccaaaagaat aaatatttta cttatctgaa atgagaaaat
    11821 acgttttctt aagtataata cgttttccta aataaaatga aaaaactatt tcaaataagt
    11881 ttcagaaatg tcaataacat gtccttaatt aaggataaaa caaaaattta acattgtcaa
    11941 cgaaaaatat ctaaaatatt acatattttg aatatattgt aatatagctt tattatgaaa
    12001 aaattgttta aatattgtag gaataatagc taaaaagctt taaaattagt aatataaaat
    12061 tgaaaaataa taatatagag ttgagaaata gtaaattgaa attttaaaat aaatgagtgg
    12121 aagtagctta gtaaagtatg gaaatgaata ttacaataga aaataaataa tatgtaaaga
    12181 ctttaaatta attttaagta ttaagtacaa atactaattt tgatatagaa attgaataaa
    12241 gtaaatgata ataagcaaat gaattaagat gataataagt gaattgatat agaaataagt
    12301 aaattaagta agacgataac aagtgagata ataataaatg aattgacata gcaataagta
    12361 aattaagtaa gacgataaca aatgagatga taataagtaa attgatatag aaataagtaa
    12421 attaagtaag atgataacaa gtgaggtaat aataagtgaa ttgatataga aataagtaaa
    12481 ttaagtaaga cgataacaaa tgagatgata ataagtaaat tgatatagta ataaataaat
    12541 taagatgata atatccaaag tgagaaagat aatagaaagt aaataaaatc atgtaaaaat
    12601 aatttagata agattctaag attttattta tttaaaaatt agtatgatta atttagatta
    12661 aataaattta ataattttag taggtggtaa tgaatgaaaa taaatagatt aacagaaata
    12721 atagtaattc tattaaataa aaaattagta acagctaaag agttggcaga tagatttgaa
    12781 gtttctacaa gaaccatata tagagatata gaaactctgt ctatgtcggg agtaccagta
    12841 tatatgacga aaggcaaagg aggagggata tctttaatag aagaatattc tatagacaag
    12901 gctatattgt caaaaaaaga taaagagagt ttaatagttg cactgaaaac attacaggcg
    12961 actaagtatc cagaaataaa ttcagtagta aataaaattg gctcaatatt tggagagcag
    13021 aatttttcaa actggattga aatagacttt actgaatggg gaagtaattt taatgaagat
    13081 gataaattta caaaaataaa agaagctatt ttaaggcgta atactataaa ttttaattat
    13141 gtaaatagtt tgtcaagcca gacaaacaga actgtcgaac cactgaagct tatgtataag
    13201 agtaagactt ggtacttgta tggattttgt aaactaaaag atgattttag gatatttaga
    13261 attagtagga taaggaattt atctataaag gatgaagtat tttcaagaaa gataatagaa
    13321 gaagtatgtt tgaatgattc taaagtaatt aaggaaaata ctataacgtt gaaattgagg
    13381 tttaaagaaa agatgctttt tagagtattt gatgatttta ataaggattt aataactaaa
    13441 aatgaagatg atacatatga tgtaattaca gaatttccta ttggtgaatg gatttatgga
    13501 tatattttat catttggtga caatgttgaa gtacttgaac ctaaagatgt tagggataat
    13561 gtaataacta gactaaggga gttgtctaaa atttattcat tataatataa tgctagatta
    13621 aagattttat taaatatata atatcttgca tttagtatat aatatctcac tgagtttaat
    13681 tatattaaaa atatgacata aaggtgtcgt atattataaa ttataattga attagttcaa
    13741 aataaaatag attaataaaa acaaagtgag gtataaagta tgaattatga aatagtagag
    13801 ataaatgaga aaatagttat aggaatatcg aaggagacta caaataaaga tggacaagcg
    13861 gtaaatgata taggtgaatt gtggaaaaaa tttatgggca aagggatata taatgctata
    13921 aagggtaaaa agaatgacaa gacaataggt ctatatacag attaccaggg tgattttaca
    13981 agtccgtata gctttgttgc atgttgtgaa gtaaatagta attctgataa agagaagaat
    14041 ttagaagaat taaacactaa taatactgtt ggagaaagta ttattagtaa agttatacca
    14101 gcaggtaagt atgcaaagtt tgttatagta ggaggtcaaa aagaagtagg agatttttgg
    14161 tttgagtttt ggcaaatgga ttttgatagg acttatataa gtgattttga agagtatcaa
    14221 tgtaatactt ttgatacaaa gaaacaagaa attcatattt acataggaat aaagtagatt
    14281 tgttattact atattgaatg aaatattgag tatagtacat ttattataga tagttttatt
    14341 atttataact ataagcagta aatctgtaaa agatttactg cttttttagt aattaatagt
    14401 atcagtatca ctttgaatct tcagacatat tcttcattta atattgttta ttgataaatg
    14461 atacttgtaa atataatgat ttgttattta atttaaaata tgaaaaaaag tattataaac
    14521 acat
//
LOCUS       DAYATN010000101        14445 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.101, whole genome shotgun sequence.
ACCESSION   DAYATN010000101 DAYATN010000000
VERSION     DAYATN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.101"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     regulatory      complement(351..434)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(667..1509)
                     /locus_tag="ACGI8A_002713"
     CDS             complement(667..1509)
                     /locus_tag="ACGI8A_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0180703.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1761..3545)
                     /gene="pepF"
                     /locus_tag="ACGI8A_002714"
     CDS             complement(1761..3545)
                     /gene="pepF"
                     /locus_tag="ACGI8A_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFH0180704.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3611..4276)
                     /locus_tag="ACGI8A_002715"
     CDS             complement(3611..4276)
                     /locus_tag="ACGI8A_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFH0180705.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(4427..4588)
                     /locus_tag="ACGI8A_002716"
     CDS             complement(4427..4588)
                     /locus_tag="ACGI8A_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HFH0180706.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4666..5175)
                     /locus_tag="ACGI8A_002717"
     CDS             complement(4666..5175)
                     /locus_tag="ACGI8A_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFH0180707.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            5381..5599
                     /locus_tag="ACGI8A_002718"
     CDS             5381..5599
                     /locus_tag="ACGI8A_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFH0180708.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5617..7563
                     /gene="feoB"
                     /locus_tag="ACGI8A_002719"
     CDS             5617..7563
                     /gene="feoB"
                     /locus_tag="ACGI8A_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFH0180709.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFVMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(7742..8797)
                     /locus_tag="ACGI8A_002720"
     CDS             complement(7742..8797)
                     /locus_tag="ACGI8A_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFH0180710.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8845..9645)
                     /locus_tag="ACGI8A_002721"
     CDS             complement(8845..9645)
                     /locus_tag="ACGI8A_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFH0180711.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9638..10429)
                     /locus_tag="ACGI8A_002722"
     CDS             complement(9638..10429)
                     /locus_tag="ACGI8A_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFH0180712.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(10469..10891)
                     /locus_tag="ACGI8A_002723"
     CDS             complement(10469..10891)
                     /locus_tag="ACGI8A_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0180713.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10907..11386)
                     /locus_tag="ACGI8A_002724"
     CDS             complement(10907..11386)
                     /locus_tag="ACGI8A_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFH0180714.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(11518..14274)
                     /locus_tag="ACGI8A_002725"
     CDS             complement(11518..14274)
                     /locus_tag="ACGI8A_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0180715.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDIIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4685 a         2483 c         1636 g         5641 t
ORIGIN      
        1 agtcattttc acgtataaat ttattatttt tctagaaact tgtctattgg ctagtttgag
       61 tattgattta tatgatttat atggttataa tataaaataa attgtattat tttggtaaat
      121 atattgttac aaaaggttta tattctgcat ctcaatgttt ataaaaactt tattttctta
      181 ttataatact attattcttt taaaaaaatt tcccttttcc aacaaatggc tactttaaaa
      241 gccaaaattt tacctatcaa taaaaatctt aacgactaat actcttattt tcaaattttg
      301 caacatattt gttaatttta aaacaaaaaa agactatgca atataaaaat tatattcata
      361 gccggttgca ccactaactc catatatctc tagatgccca ataataagat atacttctca
      421 gtttctaaaa tatttttatt aaaaatcttt tatatatttt cataaatttc ttttttattc
      481 taacattgta atattatttt gtcaatattt ccatattctt agagataaga tactaaaata
      541 cctattttat ctaaagatat ttatatttct acattcaata atagacaata ccctgcaagt
      601 taaagcatca tttttatgag cttatctcac agggtattat atgtttactt tatattataa
      661 ctcttgttat tgattaactt taataaatcc tttttctgaa acaagtgctt gtccttcatc
      721 acttaatata taatctatta aagctttacc tttttctgac aaaccatctt ctttattaac
      781 taatataaat ggtcttgata ttggatattt attagctatt acatttgcat cagttgcttc
      841 aacatcattt atttttaaac tatttacttt ctcatcaaca tatccaaatg atatatatcc
      901 tatagcattt tcatttcctt caactgtagt ttttatattt cctgaaccat cacttatttg
      961 tgcatcttta gttagttctt ctgaatcaaa ccctacaatt tcttggaatc catctctagt
     1021 tcctgaacct tcttctcttg atactattac tattggtgca tcatttcctc ctacttcttt
     1081 ccagttagta atctttcctg tatatatacc tttaacttgg tccattgtca gattttttac
     1141 tgagtttgca gtattagtta tcaatgctat accatctatt gctattttag tttcctttaa
     1201 tccagcactt ttttcttcat cttttaaatc tcttgaactc attcctattt cagatgttcc
     1261 ttctatagca ttttttatac ctccagaaga accaatctgt tgtacttcta cactaacacc
     1321 ttgatttttt tcttgaaact tttctccgat agtttccata agaggaccta ttgaagttga
     1381 acctgaaaca gaaactttgc ttgaatctcc tgaatcacct gaacctcctg agttagaact
     1441 acatcctact gctaaagctc caactaacat tgtactagct aaaagaacgc ctattttctt
     1501 tttaaacatt ttacttaccc ccgcttatat tttaattttt tgttgtcatt tttctatatt
     1561 ttaataatac agattctttg ttaaatgaat attagagcag ggttaagtaa atgtaaatat
     1621 tgttaaatac atgtaaaaac ttaattttat atgatttatt cttaattaga ttaacttttt
     1681 aaagttactt taggcagata taaacataat acaactcata ttatcagaag tcttcattga
     1741 agctatcaac ctatcacatc ctataattct ttttctagtt tattaactaa ttcagcaaat
     1801 acatttaagg cttcctctat agattctttt ttcgtcatat ctacaccagc agattttaat
     1861 tgttctagtg ggtattctga ccctccactt tttaagaagt ttatatatct atctactgca
     1921 gtacttcctt ctgaaagtat ctgttgactt aaagcacttg cagcagaaaa ccctgtggca
     1981 tacttatata catagaagtt tgaataaaaa tgaggtattc tcgcccattc tattcctatt
     2041 tgtttatcta ctattgcaga ttttccgtaa tacttttcat ttaatttgta gtatatatta
     2101 gtaaattcat cagcagtaag tggttctcca ctctctaccc tttgatgtac aattttttca
     2161 aattcagcaa acatagtttg tctatgaact gtagttctaa attgttctaa ataataattt
     2221 aataagtata tagtttcatc cttagactta gaattttcta ataaataatt tataagaagt
     2281 aattcattta atgtagatgc aacttctgca acgaatatct tatagtccga atataaaaat
     2341 ggttgacttg ttcttgaata atgactgtga acagagtgcc ctaattcatg aattaaagtg
     2401 aatagagaat taaggtcatt tttatagctc attaatatat aaggatgtga atcataactt
     2461 ccccaagaat aagctcctcc ttttttacct tcattatcat atacatcaat ccacccttca
     2521 tcaaaagcac tttttatgac cttcaagtat tcttctccta aaggtttaag tgcctctaat
     2581 atgatatcct gtgctttatc atatggtata tccatgtcaa atttatctgt caaaggaaca
     2641 tacaaatcat acatgtgtat ttcattcaat cctaaaaatt tctttttaag ctcaacatat
     2701 ttatttagat taggaatact tttatctata gctgatataa gattattgta aacatctaaa
     2761 cttacatcat ctgaaaataa agacgcttct attgctgaat tatactttct agtttttgcg
     2821 taaaatatct ctgattttat tccaccataa agcgttgagg caaatgtatt tttatatttc
     2881 tcatatgttg aaaattcacc ttcaaaagcg tcttttctaa ctctactatt cttacttttt
     2941 ataaataaag agtagtttga atgagaaagc ttaaccttat tcccctcctc atcttctatc
     3001 tcaggaaatt ccatatctgc atatgctaac atatcgtata cattttctgg aattgtcgat
     3061 aattctgatg tagctgcaag tatttcttcc tctcgttcac tcaaagtatg tggtttatct
     3121 ctcaatatat cctttatata ttttgcataa aaggatagct tctcatcttt taagtattca
     3181 cttaacttat tctcttctat cgccagtatt tctggaacta tataagaagt agccacacct
     3241 aaatcagtag ataacatttc tgtcttagtg gctcttcctt gattatcatt aattctagta
     3301 tcttcatgtt gtttcatatg agtatataca tataaatttt gaacaatcct agatgcattt
     3361 tcagagatat ttaatacttt atataagttc tccttactct cagaaagctt ccctttatat
     3421 tcttttactt cttggataag attttttact ttacttatgt ctttttcaat ttcctcttta
     3481 ctggagtaca tcttatctat cttccatttg tacttttctt ctattgtact tctatcagtt
     3541 cccatatcat acacctctat tttaatttgt tttatatttt attcacttta caaaatatat
     3601 tgtatatatt ttagtccaaa taatctacat ttattactcc ttctatcata tttatttctt
     3661 tcataacgtc gtgtaagtgt attctcttag cataagatac aaaaaaatta accttccagt
     3721 attcttcatt acgacattct atttccatat ttcttacgat aagttgattt ttttcaaatt
     3781 tatcatataa aaaaggtata gaatccaaat tgcttaattt taatatcaat acattctttt
     3841 cttggctaaa taaaaacgta ttgattatcc taagcacaaa taatgctatc attacaaata
     3901 atccacctag aattgccaat tcataataac catatccaac tgctatacca atacatgcag
     3961 ttgcccacat cccagcagct gtagtaagcc cttttactcc acctttattt tttaaaattg
     4021 ctcctgcacc taaaaaacca attccagata atacttgagc aggcattctt gctgggtcag
     4081 catttgtata caaattataa tcaaagaata attgaatcga agttattgaa acaatacaag
     4141 aacctattgc aattagtata tgtgtcctaa atcctgcaaa ctgtcttatt ttttctctct
     4201 ctaagcctat tattcctcct attattagtg ctaatagtaa ccttattgct atctccttac
     4261 ttcctatatc aatcataata taaaaccctc ctgacttaat tacaacttat gtcctacaga
     4321 taatttacac atcagaaata ctattttata tcttaatatt attattaata tatgtaaaat
     4381 atagccatct gttaattttt gatacaaaaa aacccacaac tgtgggctat ttgcttaggt
     4441 acatgtattt atttattaat atatctattt tcttacttga ttctactaac tcttcttgaa
     4501 atatttctgg atattctatg tatgtattta tctcttccct aactgcttca atctcatttt
     4561 ttaacgcttc taattcattg ttcgccataa taatcaccac tattacaaat ttgtatattt
     4621 ttatatacat tttatcaaac aatattatat tttgtcaata ttatgtcaat aaaacttact
     4681 ataaaaatgc tttaatttag gtattgtata cctggatact aagcctacaa acataccagt
     4741 tactaatgat gccattaaca taaatggtaa gtaccctata gcaagtatat tttctatgat
     4801 taaacttgca actattaatt gacctatatt atgccctatt gcacctataa tgcttatccc
     4861 tattagtgat aaatctttta ttttactcgc taaatacata aataaaagac tcagataagc
     4921 tcctcctata ctaaatagta gatatgataa agggcctgca aatattgatg acaaaattac
     4981 cctgatagta acaattataa cagtatcttt gacacctaac aacattaatg aaattaaagt
     5041 tataatattg ctcaaaccca attttgcccc tggaaatata actataagag gatttggtat
     5101 atatgtctct aatacatata aagctaaact ataccctatc ataagaccta aaaatgtcat
     5161 ttttctagtt ttcattaagt taactcctcc aaattttatc tatcatctta cattataata
     5221 caaaatatat aaaaacacct aatttagact aaaatatttt taatctattt agaataaata
     5281 ttagttattt caatgtgcaa ttacctctat aaatcttgac tttttgtatc atacttgtta
     5341 caattttaat gataatgaat ttcattagga ggtgttaata atgactgttt acgatttaaa
     5401 aataggagaa aaaggattta ttaatagtat agatggagat attaaattat ctaaaagatt
     5461 gcttgcactt ggatgtataa atagtactga aatagaagtt aaaaaggttg ccccacttgg
     5521 agacccaatt ataattagat ttagaggctt tgaccttgcg attagaaaat ctgatgctaa
     5581 aaatatttct ttacaataat agaggaggag ctacccgtga ttaatgtagc attattagga
     5641 aacccaaatg tcggtaaaac tactgttttt aatctactta caggttcaaa tcaatatgta
     5701 ggtaactggc ctggtgttac tatagaaaaa aaagaaggat ttcttggaaa agagataaag
     5761 gttgtcgatt tacctggtat atatgcaatg gatactttct caaatgaaga aaaggtttct
     5821 aaatcatatt tagaaaatga agatgttgat gtaatagtaa atgtagttga tgcttctaat
     5881 ttatcaagaa acctttactt aacaactcaa cttatgcaat ttaacaagcc tatagttata
     5941 ttgcttaata tgttagacat tgcagaatct aaaggagtta atatagatgc caaaaaatta
     6001 agtgaagaac ttggagtaat tgttgttcca attatagcaa aaaagaaaaa tggtgttgat
     6061 aggatagaag aagagattaa gggagctacg caaaaagctt ttatatatca gaaaaacttt
     6121 ggaagtgaaa cagaaacata taaaaaatta gaatccattt tatcgagatg tacaaaaact
     6181 acatatacac aaaagaaatc tatcagtgat aagatagaca atatagtctt gaatcctata
     6241 ttagcatatc cgatatttat tggtgtattg ttcctcttat ttaaatttac ctttgactgg
     6301 gtcggaggac cactacaaga gggatttgct agtcttatag aggcttatat ttctgcacct
     6361 gttagtgata tgttatctaa ctctagccca tggtttaaat cacttatagt tgatggaata
     6421 attggaggtg ttggtggtac tttaccattc ttccctttaa tatttacatt attctttggt
     6481 atatcgttgt ttgaagatag tggatatatg tcaagaacag catttttaat ggataaagtt
     6541 atgagaaagg ttggtctatc tggtaaagct tttataccta tagttatggg tatgggatgt
     6601 tcttctccag ctataatggc aactagaaca ttggaaagcg aaaaagatag aagagttaca
     6661 gctcttattg caccacttat gacttgtgga gcaaagctac ctatatatgc actatttgta
     6721 gctatattct ttccacataa tgctgcttta gtaactacat cactgtattt ggtgggtata
     6781 gttatggcaa ttgtagttgc attattctta aataaaacag cctttaaaac agaggctgaa
     6841 ccatttatat tagaattacc agaatataga atccctacta tagatgcact tattaaaaat
     6901 acttggaata aatcaaaagg tttcttgata agagttgtta ctgtcatgtt tgtaatgtca
     6961 gtcgtaattt ggggactttc ttcattcaat gtttttggat atacagaaga tataaatgta
     7021 agcttcttgg ctactctagg tcatattata tctcctatat tcaaacctct tggttttgat
     7081 gattggagaa cttcagttgc tattttaagt ggtcttggag caaaagaaat cgtagtaaac
     7141 tctttaaata tactatatgg taatctaact gttgtcttac caacaatatt taatggagtt
     7201 actgcatata cattcttgat atttactgct ttatatactc catgtatagc tgcacttgca
     7261 actatgaaaa aagaatatgg caataaaatg atgtttacat catttgcata tcaatttata
     7321 ctagcatgga taatggcttt tatagttaaa agtataggtg gagtactatt tatgggtaat
     7381 tcaataatag aagctttgat aggtggaata attatagtcc ttgcactctt tattctgttt
     7441 aataaattca agtctacaaa aaacggaaat gtctgtagtg gatgttctat ttgttctgga
     7501 tgtccaagtg ctaattcttg ctcaagtaag gaagacagca ataaaaaaaa taatgcacac
     7561 taatatataa atattttagt ttaaaataag ataaaagtgg gtgtctcaaa attaactttt
     7621 tagttcatga ggcactcttt tttatagaaa atcaaatata ttttcatcat ttcaacagta
     7681 aaaaagagct tatctagtaa aaaagaggct tatctctatt ttgagacaac ctcttttttt
     7741 gttacttatt cagtttcttg ctacctaact tcatgtggaa cctagtatct tttggaacac
     7801 ttgggtctac acctcttaat gtaggtattt ccccacatat tagttgtata ggtactataa
     7861 aattaaatgt ttgatagtaa ggattatctg tgatatcaat ctttaaattt ttatcatttt
     7921 ctgtaacatc tctacctata gcaaatacat tttccgtcca accagaaagt acatcaatca
     7981 tcttagttac tctaggttct tttcctgtat ctagtataaa tatagtagaa tcactattta
     8041 tagcattata tataccatgt ataaactctt caaactcata cccagttaca ggtattctca
     8101 ttgtttccaa taattttaat gctgcttcta atgtatctcc atatatatca gagtgaccta
     8161 ttattcttat ttctttagaa ttaactaact tttctttgtt tctttcaatc cattgtttag
     8221 agagcttata cactgcttca aatctattaa ttgcgtctaa tatcttattt atttcctcat
     8281 tgtacttttc actagatatt ataccttttt ctctagctat ttgaagacct aataacatta
     8341 agtttaactt tgtacaataa tatccttttg ttttagctcc agatttttct tcaccacaat
     8401 ttactgttaa tatataatca gaaatctcat ctattaaagc attcttacaa cctgccatag
     8461 atgctatttt acagccctta tcttcagcta acttcatagc gttgtatgta gaataactac
     8521 tcccaccttg agatacacct actactaggg tattttcatt atcaaacttg aatgtatctt
     8581 cagttatcat aaatggatac attttcacaa catctatatc taataaattt tgtaaatatg
     8641 gttgtacttg cactcctgag tgatagctag tacctgaacc tgttattata atctttttta
     8701 gatttgttct cttaacttca ttaaataagc tatctgcatt agatataatt tctctcattc
     8761 taactggtgt ttctaacata taatcttgta ttgtcatttt atacaccttc cttatctata
     8821 tattcaataa attataatta atttctataa caaacctata gcatgtaaag cgattcctgt
     8881 tactatagtg cctatagtta attttgttac acttaccttt ctctttaaca tgtagtatat
     8941 tacaaatgca aaggttaaag gtaacatatt aggtattatt ttatctagta tatcttgtaa
     9001 aattacttct gcaccattta agttgaattt cagaggagta gttatactca acatagttgc
     9061 aaccattcct cctacaacga ataaaccaac agttgtagcc atagacatta ttttctccat
     9121 aagtccctct ctttgtattc tttcaagaga atttacccct acttgataac catacttaag
     9181 tccaaatatt ctaagggcaa atgctggtat attgtataat agtaagaata atagtggtcc
     9241 aaagatatta ccttcttttg caagtgatac tcctactcct gctgctataa tcctaaatgt
     9301 tccccagaat aatgaatcac caattcctgc aagaggtccc ataagagctg ttttaacagc
     9361 actaattgaa ctttcatcaa attctggatt attagcattc tcttcttcca tagctgctgt
     9421 tatacctaat atagcaggaa ctactactgt cgttgtatta aaaatctcca aatgcctttc
     9481 tattgctttt gcttgatttt ctttcttatt gtatagcttc tttattgcag gtaacattga
     9541 ataacagtaa cctaagtttt gcattctttc gtaattaaat gcaccttgaa gagcaaatga
     9601 acgccaaaac atacttctta attctttttt tgttaattta ttcaaagtca atctctcctt
     9661 ccgttaaagt attgattgct aattgaggat tttgattatt ttctttgtta ttgatataaa
     9721 agtttagtac aaatgctaca catactccaa ataaagctat agcagttata tctaatccac
     9781 cataagatgc taacaaaaac cctataaaga aaaatacaga catctttttt gaaagtaaca
     9841 tatccataag caatgcaaaa cctactgctg gcaacaatgt tcctgcaaca cccagtccgt
     9901 ctaatataac tttaggtatt gcatttatca atgctgtaac tttatcagca ccaatcataa
     9961 ttgctaaaaa ggttggaact gatgtactta aaaagaataa tattactggc aaccacatca
    10021 ttatggtaac tttcttaaaa tcagcttgtt tagcatagaa aactgccttt tgagaaaagt
    10081 acgaatttat tattctaact aatactccaa gagattgtgc taagactgct actggtactg
    10141 ctactgctaa agcaacttct gcaccattac ctgataatat agcaaaagct gtacctaata
    10201 cacctcctgt tactacatct ggaggagtag ctgcacctat tcctgcaatt cccatccaaa
    10261 ttaattcaag ctgtgcacca atcataattc ctgattttat gtctcctaaa actaatccta
    10321 ctaacatacc tgtaactatt ggcctatcaa gcatagattg accaaatata cgcccatcta
    10381 gtataccaac tcctgctata atagctaaca tagtagcttg tactaacata ttgaacctcc
    10441 attttaactt agtttaattt acattaaatt acattacatt tttgattttg ttttgatttt
    10501 ctaataactc atttacataa acaattccat tttttgcttc ctctaatcct gatttaacta
    10561 ttagttttgt atcctcctca actaataaaa gtatagataa aagcattggt aaatttactc
    10621 ctgtcaataa ttcaaatttg aacccttgta aaagtaagtt tgtacatatg tttgccacac
    10681 ttccaccaaa aacatctgtt aagacaacaa attcttcact atctttatta tttgataata
    10741 tacttctaat ttctttctca gcatcatctg ctgatttatc cgttgtcata caaaaatact
    10801 caatattagt taatgaacca attattaatt tacttgtgtc aacaagctct ctggacaatt
    10861 ctccatgtgt tgcaattaga aattttttca ttctctttct cctaaattat tttattaaat
    10921 ctgatagttg tttctttttg tctctaggta acattctaaa ttctatatca atccctaaat
    10981 cacctatttg tttaaagtta tttatatcat catcatcaac agcaactaaa tctgtaagtt
    11041 tcttcttccc ttcctcatat cttaatcctc ctacatttat ttcttttaat actcctcctg
    11101 aatttttagc tacaactaaa gcatctttgg tattttttac taacactaat acattgcttt
    11161 tctttgaaga agcaaatttt tctactattt ctccagattc ctctaccccc cttatatata
    11221 aagttacatt gctaggtttc gctaagttca gtgacatagc tttcatttgg tcattttttg
    11281 cttcatcatt tgcaactagt attacattta tacctagtga tgttgcccat ccatatgcaa
    11341 cctgaccatg tatcaatctg tcatcaattc tcatcaaaga taccattttc cccaaatcct
    11401 ccatcataca ttaaaatttt tattgtttat actctcattt atctataaaa gcaaaaagta
    11461 tgccaaatac tggcatactt ttttaaattt cattcactta ttttgtaatc ttgtatttta
    11521 ctgtggtaga ccatagataa tattataaat atatcctatc tctgaaattg gtatttgcac
    11581 actataactc ttttctagca cactaaatgc attttttatt agttttactt cactatgatg
    11641 gtttttgatt aacaaatcca tatcagtata ctcttcaatt tcagtttgtc taactaatct
    11701 ttcaatcata caacttaagt gaacatataa agaaatcttt ttatcatttg aaagtttttt
    11761 cttttgaata ttttctatag cattaattcc ttcttctatt ctctctatta tcttttttga
    11821 atctaaaata gttagactat ctattactct aattaatgaa aaattcttta caatagaatc
    11881 atttatgttc tttatttttt cattttctac tactctgcta aatattctaa aaacatcatc
    11941 ttctcctttt cctgatatta tatcctctaa taatataaaa tctaccccat tcacatttgg
    12001 attggcagtt cctactatag ccatcacatc atacatttca aaaactggtt ctttatctcc
    12061 catagaaaca agactatcat aatcataagg tattattttg acatcaacta ttccaattaa
    12121 actttcggaa agtagatgtt ggatttttat agcagtacct atccctgtaa aacaagatga
    12181 tattatagca tattctttat ttttttgagg ataatacaat tgtatattag gtttagtttc
    12241 ttcatccata atttctgcaa tctcttcaat gtttttacct tgcattaata aatttcctac
    12301 ttctagtgca gaaatagtgg atatttggtc tataaataac attgggctat ttatcttatt
    12361 ctttaaatct tcatatataa gattaagaga gcccatatca aataacacaa ttaaaccatt
    12421 ctttatccta tctccctcta ggtatctaga catttgtttt gatacatccg caatagtagc
    12481 atctataggc atatcaatag ctgtaaatat atttttaccc aacatccgat tacatacatt
    12541 cgccatacta ctggcagtag catatccatg tgctataata gtcgcattaa cttgattaaa
    12601 tttcattctt ataattgttg atttaataaa tataatcatt atgattatat ctacattatt
    12661 aagatacata tccaatctat tcgttattaa tgttgatatt ttacttatta tagagtattc
    12721 tttgtaactc tcttttttta aatatattag taatttattt tctaaagctt tatactcatc
    12781 agacaattga taagattcgt ccttcaaata aagataagac gaaaatgcca atacattatt
    12841 cccatcatat tttatattgt tattaacttc taaatttcta aatatatcct gcattatcga
    12901 tattataagc tccataagta cgttattatt ttcatcatta tttgaaaaga ctaaattgtc
    12961 cattagtcta atcattagat tagaactctt tttactaaat ttttcctttg ttatttcatt
    13021 tttttctagt ctatcaaagt aatctaaaca ttttatgaaa ctttgataga ttatatttct
    13081 tttatttctt ccatttgaca tttcaatact aaattgttta ggagaaaaga taatgctttt
    13141 gcttttacca ctttggaatt gaaatatatc tacattttct atataaagat ttctaggaag
    13201 gtcttgaaga gtaacaaaaa tatttccatt tcttctttga ttttttgcat aagctcgtcc
    13261 acatgcatat tttattacat ttttacattc acctatattc cccttgaatt ttatctttaa
    13321 aatcgcattt aatgtaattt gagttatctc tataggcaaa ccaagtacct tactttcttc
    13381 ataaataaaa tgttctacaa tctttctttt ttcaatagtc cctctttctt ctatattggg
    13441 aacataaata tttatgggta ttcttcttaa aaaggtttgt aaaaattcag atagactttc
    13501 tgttgtagca aatattaatc ttaccctagc ttttctaatt actccattct ctccaatcct
    13561 agaaaacgta cctgtatcca taaatgtaaa taacttttcc tgaccttcac tatttaatct
    13621 atgtacttca tctaaaaata aaacaccacc atctgattgt tctattagac ctgctctaga
    13681 ttcatttgct ccagtatatg ctccttttac atgcccaaat aatagactag acaataactc
    13741 caaattatta taatactgcg cacagtttaa cacattgtat ggagaatttt tttctaatac
    13801 attactgctt acactatatt tgtatattat cttggctaaa aaagtttttc ctactccact
    13861 ttcacctgtc agcattattg taagtccgcc atttggataa agtacagcgg tttttatttg
    13921 ttcgattact tcttttaagc tattatcata tcctatgata ctattaaaca cagtctcttg
    13981 ctttaataat ttacactcat ttttaagttc atctaaattt tcataaatac ttttttttgt
    14041 aggtcctact acatcattta ttttattttt aggtaaaaaa ataacaggtc tactgtttat
    14101 ctttataaga ttatcagatt ttactaaatc atttaataat ctacttgatt gagttctact
    14161 taaattaaac ttttttacta tatcttctgt tattatgcca ctttcttttt taagttcatc
    14221 aaagttgtta aataatacac attcatttac aaaacttaaa atttcatccc tcatgttcaa
    14281 cctccaatac aaatatcaat aatactataa tattagtata tcatttatat atttatgaaa
    14341 aagataatta tattcctatc ttgattctct tttgtaagtt tgtaaaatca aactttttat
    14401 aagtataaaa tctagatttt ttaacataat ctcaaggttc ttatc
//
LOCUS       DAYATN010000102        14129 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.102, whole genome shotgun sequence.
ACCESSION   DAYATN010000102 DAYATN010000000
VERSION     DAYATN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.102"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            402..1805
                     /locus_tag="ACGI8A_002726"
     CDS             402..1805
                     /locus_tag="ACGI8A_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0180716.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2282..3304)
                     /locus_tag="ACGI8A_002727"
     CDS             complement(2282..3304)
                     /locus_tag="ACGI8A_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0180717.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3339..4937)
                     /locus_tag="ACGI8A_002728"
     CDS             complement(3339..4937)
                     /locus_tag="ACGI8A_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HFH0180718.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4979..6244)
                     /locus_tag="ACGI8A_002729"
     CDS             complement(4979..6244)
                     /locus_tag="ACGI8A_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0180719.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAE
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6386..7813)
                     /locus_tag="ACGI8A_002730"
     CDS             complement(6386..7813)
                     /locus_tag="ACGI8A_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HFH0180720.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLFASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQIYMGVEE"
     gene            complement(7851..8858)
                     /locus_tag="ACGI8A_002731"
     CDS             complement(7851..8858)
                     /locus_tag="ACGI8A_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFH0180721.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(9055..10377)
                     /locus_tag="ACGI8A_002732"
     CDS             complement(9055..10377)
                     /locus_tag="ACGI8A_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180722.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10735..12375)
                     /locus_tag="ACGI8A_002733"
     CDS             complement(10735..12375)
                     /locus_tag="ACGI8A_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0180723.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12531..13187)
                     /locus_tag="ACGI8A_002734"
     CDS             complement(12531..13187)
                     /locus_tag="ACGI8A_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180724.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(13213..13914)
                     /locus_tag="ACGI8A_002735"
     CDS             complement(13213..13914)
                     /locus_tag="ACGI8A_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HFH0180725.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     KKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
BASE COUNT         4801 a         2658 c         1671 g         4999 t
ORIGIN      
        1 aacaaatcct ataattatat tctaatatac tacctactac tttgcgctac aacaatttct
       61 ctatttatat gatttgtaaa atatagtaca acatttttta tcattttcat ttcaactaaa
      121 tttgtaatat tttattcaat acttaataaa tacttaatca atacatgctt tataatactt
      181 tactcaatat ataatcaata cataatttat aatttgtaat actttattca atacataagt
      241 agaaatctct atatacataa attcattttt tcatacatct tacaaattta tttttattta
      301 gataacatgt atattttttc catgaaaaat aaataataaa aaatagtaaa tatggaaaat
      361 ataaaattga ttaacaataa ttaattacct gaggtgtgaa tatgaaaatg ctaaacaact
      421 tttttaagaa aatcaacaat ccagctcctg tctttgcaca gacaaaaatg gaaagagata
      481 atcaaacaga tgattcactt tctacaaaac caaagactac atttagagac gttgctggat
      541 tagacgaagt aaaagaagaa ctatttgaaa tagtcgattt tatgaagtca ccacaaaaat
      601 atcaaaaaat gggtgcaaaa atacctaaag gagtattatt ttatggtcct ccaggaacag
      661 gtaaaaccct acttgcatct gctgtagctg gtgaaacaaa ctcatcattt ttcaatgtta
      721 ctggttcaga gttcgttgaa aaatatgtag gtgtgggagc aaaaagagtt agaactctat
      781 ttgaaaaagc tagaaaagaa gctccaagta taatatttat agacgaaata gatgctgtag
      841 gagcaaagag acatcttgaa agtaacaacg aaaaagacca aacactaaac caacttttag
      901 ttgaaatgga tggatttaat aaagattcca atgtattaat aataggtgct accaatagac
      961 ttgacttatt agatgaagct ttacttagac caggaagatt tgacagacac attcatatag
     1021 gtgcaccaaa ctatcacact agatttgaaa ttctaaaggt acatacagat gacaaaccta
     1081 tagacaaatc tgtaaatcta gaacttttag ctaaaaaaac tcatggtttc aatggggctc
     1141 atctttcaaa tatagcaaat gaagcagcta tatttgcagt tagagatgac agtgagtgta
     1201 ttacatcaga acattttgac aaggcacttg aaagagttat tgcagggcta gaatctaaaa
     1261 actctgcatt ggttgaaaaa gagaaaaaaa tagtagcata tcatgaagct ggacatgctc
     1321 ttgtaagtga tatagtagga atttgtccta tacaaaaaat atctatagtt cctagaggac
     1381 aagcattagg ttatgttctt caattacctg atgaagatag atatatttat actaaggatg
     1441 agttgattgg aaaaataaaa atattacttg ctggaaaggc ttcagaagaa ttaatattta
     1501 atcataaatc tactggtgca aaggacgact taaagaaagt cactgaaata gctaaccaaa
     1561 tggtttgtga atatggtatg agtaacttag gatttatgac tattgatgga aatgataaga
     1621 ctttcttatg tgataaggtt caaaaggaag caaatcgtat agttgaaatt tgttataaag
     1681 agactttaga gatgttaaag gataatcttg aagatttaca ttctgtttct aaattcttat
     1741 ttgaaaaaga aactatgact catgaagagt taaaagattt gataggaaaa gaagctgtaa
     1801 actaatatag ttttaaaaat ttgaggctgt ctcaaaatag agataagcct ctttttattg
     1861 ttaaagttat aaaaatacat ttgattttat acaaaaaagt gcctcataaa ctaaaaattt
     1921 cattttgaga cacttacata ttttaattac atattttaat tttgtctatt tacagaatat
     1981 tatatctaca taaaactaaa ctgctgtaaa cttaactacc tccagtaatg tttgaagtgg
     2041 tataaaagct agtagcctag tttctacact tataatttat attttacata actaaattta
     2101 gtagattttt atactcacaa tttagaacga ttgaaaactt aaaaatttct tatcagatat
     2161 ctacaactta attaggtatc tatcacttga ttaaatatct atcacttaat taggtatcta
     2221 tcacttgatt aaatatctgc catttaatta aatatctacc acttaattaa atatctacca
     2281 cttaaagtgt agaacctctt tcaatcaatt caggttttaa catttcaacg actgactctg
     2341 gattttcttc atttaataag gacttaagta tgttaattgt tttttcaatc aacaaatcta
     2401 gtggctgtct aatcgtcgtt aaattataac tcttccatga agccatttga atatcatcat
     2461 atcctatcac tgataaatcc tctggcaccc tcaacttgta ctcaaacctt gcagcatcca
     2521 ttacaccaat agctatcaag tcacttgtgc aaaaaattgc tgttggagga tttgataatg
     2581 ctaacattct cttacctgct tcatatccag aatcataatc atagtttgca gattcttctt
     2641 taaggttatg aatcccactt tgtcttaact tagagtaaaa tccaattttc ttttccattt
     2701 cttcacttgt ttctctcgta aattgaatat atcctatatt cttatgacct ttcttaagca
     2761 gatattcagc agccattccc ccaccttcaa ttgggtctac atagactgta cttattttta
     2821 taccaggtat aaaacgatta aaaagaatca caggaatatt gttttcttca caagtctcag
     2881 ccatcacctt attcatggcc gaagctgtaa taatcacagc ttctacttga aactgaatta
     2941 cttttgaaat tatactatct atactttgtt gtctaggaac tttaaaaacg agacactgtt
     3001 tacctatctc ttgaattttt tctacaaata aattgataat tctattgtaa aatggaccta
     3061 gactatcacc aactatcaat cctataacat tagtttttcc actaatcatt ccccgtgcta
     3121 ccaaattagg aacatatcct atttcatttg ctacacgaaa aattctttct cttacttcag
     3181 gttttacgcc ctttccattt gcactaccaa atgctcttga aacagatgat tgagatactc
     3241 ctgccaatct tgcaacatct tttgaggtag ccgatttatt ataatgaact ttcttttgat
     3301 tcatttagta tttctcctta aatatctagt atttcttctt aaaattgtat caaaaataat
     3361 ataatatatt ggataataaa aaaagctaat acaattaatt cttgtttacc taaagaaaat
     3421 ggcactatag atactccatc tactttatcc attccttgag aaataaggtg atttctcata
     3481 ccaaatgcag tgtatttttt aatatcatct gcattatgat ttttctttaa aatgacacta
     3541 aaaatatatc caaatatcaa tgttattgta gcacctattg ctggattcca caaccaactc
     3601 acattatatt tattagaaat aaaaaatcct aaaactaacc ctacaataaa accaatagat
     3661 actcctttat cattagcttt atatgtaaac attgctagta caaatgcacc tgtagctggt
     3721 ccagcaaagt atgagatata gttaccaaca aggtcaagaa ttgatttgat gcttcctcca
     3781 aatcccataa taataaaacc tgtcataaca actcctataa ctacagttat aatcattgta
     3841 acttttaatg atgattcttt gttatctttt ttgaaataaa actcataaat atccttcgta
     3901 aaaacagttg tcatactgtt taatatagag tcgatactag acattgctgc tgcaaacaca
     3961 cctgcaataa taattccaat ggctcctact ggaagttcat ttaaaataaa agtaatcatt
     4021 atttcatttg aagtttcaaa tgtccttccc ccataaaaaa caaataaaat tactcctacc
     4081 aatagcataa agtagtaaac taaattcata acaaatgcac taactacaaa agaatttttt
     4141 gcacatttta aagatttaga tgttaaaact ctttgtactt gagattggtc aaaacaaaag
     4201 tatcttatcc acattacagt tccaccaatt aaagtagccc aaaaagtatt tgtcactgaa
     4261 atatccctac tgaaatcaaa tgtatttaat tttccactct gttgtgcaat ttgtaatgta
     4321 tcaaagaaac ttagattcat tccttttaag gctacaataa ttactataaa tacagcactt
     4381 attactacta tcatttgaat actatcagtc catataactg ccttaatccc acctaataga
     4441 gtgtatataa ttgatacaag tacaactatt gggactaaag cataaagtga ccagccagta
     4501 atcatttgaa taataagcac tggaatatat accatagaac tagcttgaat caatgaatta
     4561 ataaaaaatt gaataatagt taatcctctt gaataatttc ctaaacgaag tcccatatat
     4621 tcatatatag aggttatttt taatttatag ataactgggg tggtaatgct cagtgcaaaa
     4681 aatattgcta aaggtactgt aatattaacc attactggat acattccatc tacatatgcc
     4741 catccaggac cacctataaa gccatttgca ctaatcatag tagctgcaac tgataaagca
     4801 attggaatcc atggcattga cctacctgcc aagaaataat cttcttgatt tttattatct
     4861 tttcctgaat aataaccaat aactaacatt aatagcaaat atcctattat aatacataca
     4921 tcaattgatg aaatcataag ccaccccttt ctaagtaaca aaataaattc ctagtatttt
     4981 attttttatt atttaacgtc tctggtaaga ataaccatac aatagcacct gctacaaaga
     5041 ctgcgattga tatgtaaaag atagtcatca tacctaaagt accagaagct gtaccagcta
     5101 tagcaggtat tgcaaacgta gatataatcc ttcctccatt aaatgacata ccagatgaca
     5161 acgaacgata tttacttggg aacaactctg tgtagtatgc tccccatgct ccagaatatc
     5221 caacagcaac acctagtaat actgatatca tatagaatgt tgttgtatta tctccaccta
     5281 attttgtata tataaaaaca aatactgctg ctaatgccat acctataaaa ggtatttttc
     5341 ttccaaattt atctgcaatt gaaccccaga ataaatatcc tactaattga ccaattcctt
     5401 gtaaactata tatagactca gccactgctg ctgacattcc tattcctgtc actaggtatg
     5461 tagttgcatt atttgaaaat gctgaatatg ctgtaaaatt tgcacctgat aaaagaatcg
     5521 ttcccaatcc aatccataag tattttccct tatacatact tatatagcta atcttttcac
     5581 cagtttcttt tcccttttta actaattcct ttgtatactc caaatcatct ggcacaaaga
     5641 agattgctaa gatagctcca actataggtg gtataatcgc agtatacatc aatatttgcc
     5701 aatttgcatc acctaaaacg ccatatactt tcccaccaaa aatcattgct aatgaaaata
     5761 aagcagttaa tatagcagct aacttaccac gatatttact tggaaacata tctgcaacta
     5821 aaggcatagc tgttccaaat acgcctccca acactactcc tgtacacaaa cgtgctattt
     5881 tccataacat aatatttgta ggagataaca ttggtataaa tgttgtcaat ccaatcagta
     5941 aagcaacgct aattaaagtc ctcttcttcc caatttttgt agaaatcacc ccaaaaataa
     6001 atgtacctgg aattgtacca aaagacataa ctgcaaatat ttcttctgtt tgttttaatg
     6061 aaaaaccaaa tcccccatct gctaaagcat aaatcattgt tggtttaata aaagtaccca
     6121 ccataaaatt gtagcaataa aaaaagtata ttaaagcaat tgaaaaaaat gcaattcctc
     6181 tttttaggtt tgaatagtct tttcctaata tgatatcatt ttctatttca accgttgttt
     6241 tcatattttt ataattcccc cttttaaatt tagtcataat tttgctaaaa gtttaaacat
     6301 ctatttttca tatacattcc ccacttaaat tttataaagc aatacttccc tatatatttc
     6361 aaaactattt atactctaaa cagctttatt cttctactcc catataaatt tgttttaata
     6421 catccatacc atcaaaagtt atccactcct cctttacttt accttgtagt atatgataat
     6481 gattgattcc cattacttct atatgctttc cagaaggctg tccaaaaaat cctatacctt
     6541 catttattcc acgtaatctc catcttacag atacatcata accttcattt ttttctcttt
     6601 gattacaagt tactctttcc acttcataac ttccatttgg aattgatgca aataaactta
     6661 caatcattcc ttgaatctca tcatagccat ttaaatcttt atcacatata aaatgtacta
     6721 ctgcattatc atgatagtat tttttaactt catttatata tttataatta taaatacggc
     6781 ttgtcatttc aagcatcatt tcgccaacag aatcatcttt tgcttgatac ttagttggca
     6841 taaattgacc atccatagat tcacagatac cataagttga ttgtaaacta agcactttag
     6901 attcacttgc ctttgccata ctctttgcta cttcctgagg gtttaatcct aattgagtaa
     6961 caatccataa attatctcta accaaccatt cttcatggat tctattgtta gttgtggcac
     7021 aatcaattac agttctaaag tttatttttc tacctgttgc tggtccaaaa ttactatcac
     7081 ctaagttagt tgctgtagac aaaacacggt gagaagataa aaaaccatca gcaccaaagt
     7141 tagaccatat tacattttga ccaattaatc ttctatcagg aaacgcatgt aatgtttgaa
     7201 gagtattgga aataacatct ttaataccaa ctaaatttga agagccacaa tgcattgtaa
     7261 cattattgtg atatgtatca taaataacac ctataccttt ttcttcccag atattatgtg
     7321 taatcttcat aatataatct acaaaatctc tatacttaga atcaaatcca ggtatttcct
     7381 gttccctttt tgtctgtttt gaaaaatcat tgtaatccat tactccaaca ggcgttactt
     7441 ctaatgaatc tccatagtaa atttttgtag atgacatatt atcttccccc ttaaatataa
     7501 cttttttatt tttttcagta agagtatttg tatttaattt atctgtatcc actgtatcta
     7561 tgttaacttt atttatgtcg gatttatttg tgtttgtttt atctatattc cctttatcta
     7621 tatttccttt atctatattt cttttatatg tgttcgtttt attctcattt gtttgatttt
     7681 catttatatt actaaaatta gtttgattca ttttagcttt gttttcttta accgtacatg
     7741 attcaagccc acttgtagaa acttcttttt cttcctcttt acttgttgca ttatttttgt
     7801 tggtagatgc cactttatat ccccctttgt tttagaatac gtatgcataa ctaagcatta
     7861 aaaatacttt tagttctctc aagaatatca agcccttgtt gtttcaacca ataaggaata
     7921 tcaattaaca cccagttttc ttgaagttta tcgcccttac gataataaac atcaacaact
     7981 tgcatatctg ctttaacctc tccacctggt aatcctaaaa aacctccaca tggtacatta
     8041 gtcagattag gccatccaaa gaaacaagca aaattacctt ctgcaaatct acatacatgc
     8101 ccattaaaag tctttccttt taaattattt ctaaatggta gctggtgttg ctcttgatat
     8161 ctaggtatag tataagaagc accaattcca cctggaccat accaaatcat atctttagcc
     8221 caagattttt caagtatttc tggtgggcat cccatagcac cactttcatt tagttcagat
     8281 aaatcatcta ccattttatt tactaatgct aaagttttta ctccttcaac ttcatctgca
     8341 tcttcaaaaa gtaatccatt atggtcaatt ggccctggat atacaaaata tgaacctgtt
     8401 tgtggtggca atggattaac tcctgcttga atcataaacc caattatatc tacaaataat
     8461 ccagttttag caattttacc atcttgaaca cagttaaatt ctgcatatct taaactaatc
     8521 attttacgag tatgtttaat gccaagccaa tcgttatcaa aaagtcccat aaaatgcccc
     8581 atactcatta cccattcact attgtcaatc tcatttgttc ctgcaatgaa aatatcttgt
     8641 cgtctttgca tattactcaa tgattttttt aaaggcttcc aaaatacatc tgctacattt
     8701 tcagtgcctt cttgttcacg aaatggatac acacctttcc agttataatc tgatttcata
     8761 tgttgcttta agacattttc aacctcatcc ccagtagcat tttctaatgc ttcaaaataa
     8821 tttcttacaa ctttttttga atcctgatat tttcccataa ttatctcctc ccagatttat
     8881 ttaagttata aattaacttt ctcatttcat gcatacgatt tcctttttaa tgatttttta
     8941 gctgttttaa gtttatttta tgaatacata tgcatttttc taaatattaa catatatcta
     9001 aaaaattgta aagaaaatgt tatcataaaa atttaatttt tgacttatat ctatttaaaa
     9061 caacagaaaa ggtttaaaac gtaatattct actgggtcta ccatacttgc tcataagctg
     9121 ttcactcact acctcacaat aaccagaatc acacaatcta agtataatcc tgtccatagt
     9181 tctaatactt acaccacata tagtagctaa ttcctttgat gtaaattcag tttttccact
     9241 atttattatt ttattgaata ctttatatac agtattagca cttagccctg tctctgtagc
     9301 aatttgataa aaacgttcat caaaactatt tttctctttg acagtaggat ttggctttag
     9361 tggtcccata atttctccat tttcaaaaac tacatatgct gcatcatttt tttgactttg
     9421 agccatctta atactctcat aagcgttaaa tttagcttca tttgctgttt ttccataacc
     9481 aattcctata taagtgttta taactgaaac ttcacgaatc atatccaaca aataaatatc
     9541 tctgtatttc tttgtttcta actcaagtat ttttttagtt gtaaaaatca taaattcact
     9601 tctactctgc tctaccaaaa caccatctat acggctattg aaatggtata gtttatcaag
     9661 aatcttaatc ctttgtgaaa gataagcgta ctcatcttcc ttcattagtg aatattctga
     9721 aggcatgttc agtttaatag caagaacaat aatctgattc tcattgtttt tctttgcaat
     9781 ataacgcatc tgaatgtttt ttatactttg caccattacg tcctcagaag gcaaggctaa
     9841 aactactgga atattatctt ttttcattat atgataggcc tcttcaagac ctgtaataca
     9901 acaacataca tcattatttt gataattata tttatgaaat tcaagcacaa actttatata
     9961 gtcctcttct aaatactttt gctctgcaaa aaacatatgt tcatccttaa attcaactcc
    10021 aatttcttta tacaatctct tcatcatagc cttatcataa gtatctatac taagtctact
    10081 aatatcgaat ttaagcaaat atgtaatttc aagcaacact ctgtacaatg ttgtttcatg
    10141 tctaggaata taatcagaca atacaaaatc tttttgtctt ttttcaaaaa atttaaatgg
    10201 cgacttacct gtaaaaagaa ctgcatccac tctacctcta tatttttcca ataaatcagg
    10261 tacttcaata tatctatcat atctaagaaa atctgcttca atattaggaa aattcttttt
    10321 acttactgct agtattttat cgattaccat acctggtcct acataaagta ttttcatttc
    10381 gtctctcctt tcctaatgat actattatct cttaaaacat tttctaaacc taccatgact
    10441 aaagccacag gactcctgat tcatagaatt ttgaataatc aattatacct aatataaaaa
    10501 gttactttaa gttttttaat tacgactatc tgttgaaaca tttttgtatt taaatttttg
    10561 tatttaaatc ttattcatta atattaatta cattttacaa ttaatatatt gttttttcta
    10621 cttaaagtta aattctttag aaaacacaag tagagtaatc tttattaatt taacaaaaat
    10681 agccttcctt aatattcaaa gaaagactat ttttataaaa gttcttaatg tattctattt
    10741 tgctatgcta agaggatttt taattacacg tccagcacat acatcagttt gctttcctcc
    10801 tgccaatgca attttaccat tcaccaacac atattccact cccactgtat actgtttagg
    10861 attactataa gtcgctttat cttctattgt atctgggtca aataccacaa tgtcagcaaa
    10921 ataattttct ttaagtagac ccctttcttt ataaatgcct aagcgttttg ctgaaagata
    10981 agttatcttt ttaactgctt cctcaaaagt caacatacct ttttctctaa catagtaaga
    11041 gaaaattcgt ccaaaagtac caaatgcacg aggatgaggc tttccaagag aaagtaaacc
    11101 ttctgtagaa aggtccatac tatcagaacc tatacatgac tttggatggc aaacaacctt
    11161 atctgtatcc tcttgacctt gtgtatgaac aactacttga attttacttt tttcttcaat
    11221 caaaagccta aagaacgtat caaatggatt ttcaccattg attttggcta cctccattaa
    11281 agttttaccc tctacatatt tatttgcttc agtcattaca gaggctagta caacaccgtt
    11341 ccacccacct gctaattggt agaaacattg ccatccagga atcccttcct ccaactcttt
    11401 tataattctg tctctatttt cttgtacttc taatcgtttt accatatttt ctacaccgcc
    11461 ttcaaaagcc caaggaggta aaagtgcacc caaagatgtc attccagctg tatatgggta
    11521 acaatcaaaa gttacttcca aaccatctgc ctcagcttca tctagctttt taaatacctc
    11581 atcaatagtt ccaaaattct tatgacccat aacttttaaa tgagaaatat gtagaggaac
    11641 tcctgcagtc ttagctatat aaattgcttc atcaagagct tccatttctt catcaccttc
    11701 atctctaata tgagatatat agtatccatt gtatggtatc aattccttac ataattcagc
    11761 catttcttct ttctttgtgt atgctcctgg taaataaact aatcctgatg aaagagctac
    11821 acatccagct tccatagctt ttctaacttc caacttcata tttttaagtt gctctaaagt
    11881 agctggttcc tttgaaaatc ccatttcatt gatacgtaca ggtccttgtg ctacataagc
    11941 tacaacattg actgctggag aattttcagt taagtaatca aagtattctt gcattgtatt
    12001 ccaatgaagc tctaattttg ttggaattgt accaattatc ctagttccaa gataatcaat
    12061 taactcttgc ttcttctctt ctcttatcgg agcaactcct ataccacagt ttgcaataac
    12121 atctgttgtt acacctgtat ggatacggct tcttgtgtat ttatcataca caagagataa
    12181 atcagaatga gtatggaaat ctataaaacc aggtgataca aattttccag ttgcgtctat
    12241 agtctcttta gcctcttttt gacttaaatc tccaatagcc atgattttgt ctcctacaat
    12301 ccctacatca cttttatatc ttgaatttcc tgtaccatca acgattattc cattgacaat
    12361 tttaacatca aacatgcttt ttctcctttc aagacttatt tatattcata tttcttaata
    12421 aacaatccta ttcttttttc tcaacaaata attctagtct ttctccttaa taaacaatcc
    12481 tattcttttt ctcaacaaat aattctagtc tttctcctta ataagcaatc ctattctttt
    12541 gttttattgt tatcaattac aaagaagacc cttgtgataa gtatcccacc taacacaata
    12601 aacaatgtta ataatccacc tggtatacca attttttcac caaagtacat aattgctaac
    12661 gctacagcaa tcgtacatcc accagctcga atatccttac gtgcaatatc aacataaatt
    12721 gctccaaata atgctggaag tatataatta aatgaattag taacaaattc aggtaacaaa
    12781 ggaattactt tatagcctac aatggtaaaa atggtaatca tagaagctga tacaaagatt
    12841 gaacaacatg taccaattgt tccaacaact tcaccctcat gagttcctgc ttctacacct
    12901 gcaattttct gtgccattga tgctgcaggc atacgtatat ctccaacaga acctgcaagc
    12961 caacctatat aagaacctgg agctcctagt gtaggataat aagctatagg ttgaacaatc
    13021 caactaaccc cataagtcgc agcgactaac ccccaaattt gaaatataac agaaagcatt
    13081 ggcatctgac caaagcgaat acctacatag atagcgggaa taaagtttgc aataattgca
    13141 agacctatag aaattctccc tatttttatc atctttttat catacataat tatcacctct
    13201 tatccgtttt aatcacgata atataatctg tgtaccaaac ataccaatta acatggcaat
    13261 acctaaggat aattcttgta atcgttgatt ctttttaaat aaagttgtca atactagcat
    13321 aactatagct gaaataattg ctggtgtaag ttttggtatt tcaaatgttt ttatttgatt
    13381 tgaaagaagg taagcaaata atccaactgt agcacctgca agtagttttt taacccaagt
    13441 aggattatac ttcatgttca tcttttcaac aattccacgc attctatgac ccaaaataaa
    13501 tacaacaaat agccaaccta agttatttaa agccatcccc catagtgcat acgaaaaagc
    13561 ttctgctcca aatgcagcac tacctacttc taccccagat aaattggatg taagagatat
    13621 tactccaagc tctgttctag ctgctccgac atcacagagc ctcatccaag tcgtaggagc
    13681 gccaatcaca gctaccagtg ataataatgt aataacagga gatagagaag gtccaatggc
    13741 tgtaacacta gctgagcgaa ttgctgcttt aattcctttc ttttcaatac caagatttga
    13801 agcctctttt agtgcctctt tcataaatac gattgcttga acaactgaaa taattactaa
    13861 aaaactagaa acaatccaaa gacctgggct attaatcatt tcttgaaact tcatacttta
    13921 ttcctcctta aaataaattt ttcagcattt tattgtgagc tttctttctg taatcttgtt
    13981 atgtttttat attgttttaa tttattttat ctaagatata gtatctttaa taggagatta
    14041 tctgtcttaa agtctcacta aaaacattaa tacttaaata ttttattcat aattagtaaa
    14101 tatcagaaaa ttcacatatt attttttta
//
LOCUS       DAYATN010000103        13764 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.103, whole genome shotgun sequence.
ACCESSION   DAYATN010000103 DAYATN010000000
VERSION     DAYATN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.103"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            80..1873
                     /locus_tag="ACGI8A_002736"
     CDS             80..1873
                     /locus_tag="ACGI8A_002736"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFH0180726.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIAPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(1954..3336)
                     /locus_tag="ACGI8A_002737"
     CDS             complement(1954..3336)
                     /locus_tag="ACGI8A_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFH0180727.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            3594..3941
                     /locus_tag="ACGI8A_002738"
     CDS             3594..3941
                     /locus_tag="ACGI8A_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFH0180728.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            3959..4993
                     /locus_tag="ACGI8A_002739"
     CDS             3959..4993
                     /locus_tag="ACGI8A_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HFH0180729.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(5042..6091)
                     /locus_tag="ACGI8A_002740"
     CDS             complement(5042..6091)
                     /locus_tag="ACGI8A_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFH0180730.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            6366..7901
                     /locus_tag="ACGI8A_002741"
     CDS             6366..7901
                     /locus_tag="ACGI8A_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180731.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(8086..8772)
                     /locus_tag="ACGI8A_002742"
     CDS             complement(8086..8772)
                     /locus_tag="ACGI8A_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFH0180732.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            8897..10279
                     /locus_tag="ACGI8A_002743"
     CDS             8897..10279
                     /locus_tag="ACGI8A_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFH0180733.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            10681..11949
                     /gene="eam"
                     /locus_tag="ACGI8A_002744"
     CDS             10681..11949
                     /gene="eam"
                     /locus_tag="ACGI8A_002744"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016869 - intramolecular aminotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006536 - glutamate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HFH0180734.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDTCDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(12042..12398)
                     /locus_tag="ACGI8A_002745"
     CDS             complement(12042..12398)
                     /locus_tag="ACGI8A_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180735.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(12549..13643)
                     /locus_tag="ACGI8A_002746"
     CDS             complement(12549..13643)
                     /locus_tag="ACGI8A_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFH0180736.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5395 a         1734 c         2178 g         4457 t
ORIGIN      
        1 tacaaaaaat atttatgtaa taaactttac atacttatat atttagttat tcaaactatt
       61 atttcgagga ggtcttgttg tgaatataaa agatagatta agtggattaa gaaaatttat
      121 ggaagaaaaa aatatagatg cctatatgat tccatcttct gataatcacc aaagtgaata
      181 tgtaggagat tacttcaaat caagagaatt tatatcaggg tttaatggtt cagcagggac
      241 ggttattgtg actaaagacg aagctgggtt atggacagat ggaagatatt ttatacaggc
      301 agaatctcaa ttagaaggaa gtacaataaa attattcaaa atgggacaag aaggttgccc
      361 aactacagat gaatatttat ataaaaatat tcctgaagga ggaactttag gatttgatgg
      421 tagagttata tctgctagag aaggagctac attagctgaa aaattatcta aaaaaggtat
      481 aaagatagaa tatcagtatg atttaataga tagtatatgg ccagatagac ctgctttatc
      541 agatagtaaa gcatttttat tagatgttaa atattgtgga gaaagtttta gctcaaaatt
      601 agctagatta agagaaaaaa tgagtgaaaa aggtacttct actcatgtaa taactactct
      661 tgatgatata gcatggttat tcaatataag aggtggagat gttaaatata atccagttgt
      721 actttcttat gcagtaataa ctttaaaaga agtttatcta tttgttgatg aaagtaagtt
      781 aaatgaagaa atactaaatg aactagctaa ggaaaatgtt caaataaagc catataatga
      841 tgtgtatgaa tttgtaaaaa atatagacaa aactgaaaaa gttttattag atggaactaa
      901 gcttagttat acaatttaca ataatatacc ttgtgaagtt gagaaagttg atgagttcaa
      961 tccagtaatg ttttttaaag ctcaaaaaaa tgaggtagaa ttagaaaata taagaaattc
     1021 acatgtaaaa gatggtgtag ctttcactaa atttatgtac tggttaaaga aaaatgtagg
     1081 taagatggaa atcactgaaa taagtgctac tcaaaaatta gaggacttaa gaagagagca
     1141 agaaggtttc tttgagccaa gtttcaatac aattgctgca tataaagagc atgctgcaat
     1201 gatgcactat agtgcaactc cagagtcaaa ttataaatta gaggcagaag gattattttt
     1261 agtagattca ggtggacaat attatgatgg aactactgat ataactagga ctacagtttt
     1321 aggacctata agtgatgaat taaaactaca ctttacttct gtagcaagag gaatgataaa
     1381 tctttctaag gctaagttct tgcatggttg tagaggatat aacttagaca tactatctag
     1441 aagttgcatg tggaatatgg gaattgatta tcaatgtgga actggacatg gtataggatt
     1501 tgtgctaaat gttcacgaag ctccaaatgg atttagatgg agagtagtac ctgaaagatt
     1561 tgatagtgca gttcttgaag aaggaatggt aactactaat gaaccaggta tatatataga
     1621 aggttctcat ggtataagaa cagagaatga aattgtagtt agaaaagctg aaaagaattt
     1681 ctatggtcaa tttatggaat ttgaagttgt tacgttagct cctattgact tagatggtat
     1741 agcaccagaa ttaatgaata aggatgaaaa agattattta aattggtatc ataagttagt
     1801 ttatgataaa atatcacctt tcttaactga tgaagaaaga gaatggttaa aagtttacac
     1861 tagagctata tagtttgtac tagatacaat aaaaatcgag gtattataat gcctcgattt
     1921 tttattgtat attttattaa ctttaaaaaa tttttaattt gataattcta ctaatttttt
     1981 attcattata agtagtataa ttccaaatat caataaaaat gatggtatca ctatgaagac
     2041 ttgtaacata ccaagttggt ctataatagc ttgcgtatag ccagataatt tatttgctgc
     2101 aaatttgcct aaaaaccaaa cacccattag gaatgaaata tacttttcag gcgaaagttt
     2161 atttaccata ccatacccga ctggagaaaa gcaaatttct cctatacttt ggaatgttaa
     2221 aaatccaact acccaaaata cacttgcttt gtgaattgaa ttagggtctg ttcctgtttg
     2281 tataactgct actatcataa acaaaaatgc tatggctaat agtataaaac ctaagctcat
     2341 tttttttggc acacttaaat cgcctttttt agtttgagat aattttaccc atactaaagc
     2401 agagataggt ccaagaataa cacaaaggag accattgtat gaatctatcc atgaagtagg
     2461 aatttgaaaa ttgccaacag taagatttat gtgttccttt gtatagagtg caatagacat
     2521 agaggcttgg ttatatgcta tccaaaaaaa tactgtgaaa aaagctaaca ccaatattac
     2581 tattattctt tttttctcaa tcaaagttaa aggtcttgaa gcgagcattt tcttatattc
     2641 tagtgatttt cttatagact ctctcccgtt aatagggtca ttattcaaag gtttataaaa
     2701 ctttctgttt ccaaatataa agaagaataa acctattaga accgtaattg atgaaaataa
     2761 aaatacatat ctatatccat acatacttat agataatccg cttttcatag caaaccattc
     2821 attagcaact aaaccgcata ttaaagggcc aaataaagta cctagattag tagccatata
     2881 cgtaatagag aaagctccat cttttcttgg gtcatcattg gtatataagt ttccaaccat
     2941 agtatgtaag tttcctttat aaaaacctat tcctaatcca gataaaaata tcatgatgtt
     3001 tatatccatc ttactatttg ctatgtatcc tagaaaataa ccaattgaaa ccattagcat
     3061 tccaagtata atacagtttt tgggacctaa aaatctatca ctgacccatc caccaataac
     3121 tggagagaaa taagtccatg ctaaaaaaga tgcaagcatt gatgcggatt ctgctggtgt
     3181 taaccctaaa ccacctttgg agacttgttc agctaaaaac aataataata ccaatttcat
     3241 accatagtat gcaagtccta caaaggtaaa tattgaacag cagaaatata aatctctagg
     3301 ttgtttaagt ttttcttgcg tttttaaaac tgacatatgt cgagaacctc cagataaata
     3361 gtttaacaaa attttaccat actatatgag tatatgtttt tttcatatag acgtcaactt
     3421 gtaagataat ttaattaagt cgaaatatat gtaaaaatag tatcattttt gtgtattcag
     3481 taatattttt ttatgataat atatgttatt attatattaa aatacaaaca tatttatgga
     3541 aaggatatgc caatttagta gttagattga ttgtttggca ggtgggatga gctatgataa
     3601 gaaagaaatc tgaattacaa gtagaagtaa aagaaaattt aaatggtggt ataggaaagg
     3661 ttaagcttga aaacataata caaaatgaag agcttaaagg aaaaggaaga ctatttaaaa
     3721 gagtagcttt accagtagga agttcaattg gtgtgcatga ccatacaaca gattttgaag
     3781 tatattatat actaaaggga aaaggtaaag tatttgataa tggggaattt gtagaagtaa
     3841 atgagggtga tgtagtgtat acagctgatg gcgaaaaaca ttctatagaa aatataggag
     3901 aagaagatct tgagtttgta gcactagttt tatatatgta aaatatggag gaggagtcat
     3961 gacaggcaag atagaagaaa ttaaggacaa gataaataga ttttgcaact ccaatttaaa
     4021 tcaagaatac aaagatataa gctttaaaat tttgcaaaac cttttagata ataataaaga
     4081 gataattcat tcttctagag ctgatatatg gtcttcagct atactaaata tagttttaga
     4141 acaaaatctt ttatacaata agaagcatcc actacatata acaaaaaaag agttttcaaa
     4201 acaaataggt gtttctttaa atactataaa taataaatcg tctttaataa aagatgtttg
     4261 tagtattgac tttttaaatc aagatactat agctatttct aatgtagaat tttgggtaaa
     4321 agagactaat tccaaatcaa agtctgtaga tttagattta gaaaagaaga aaatactata
     4381 taaaagatat attaagctat cacaagatgc gaatagtgat attgaatcaa ttagatatat
     4441 ggaggaagct gtaaatatag gtaaaagtat gattactaaa gaagataaag aacttggatt
     4501 ttggaaaggt ttatctacca gagcctatat gatagcactt gaatcccttg ctagaaagtt
     4561 agaaagtatt aataatctaa aggagtccaa aaaagtatta gaatatttaa tagaaattaa
     4621 tcctagtgat gagcagggaa taagatacaa attattaaat gtattaatta gattaaatga
     4681 tagaacttca ataaataaat tatttgaact ttataaagaa gaaaaatctg ctacatggat
     4741 gtacagtaaa gctctttatt actttaaaaa taaaagtatg tttttggcaa atgattctat
     4801 taaggttgct agaagtaaaa ataagtacgt tggattatat ttgttagatt ggcgaaatgc
     4861 ttttggtaga gaatttgtta cagaggaaga aaaggcagaa gctgtctatt actatgatga
     4921 aaacatagtg atttggaatg aagttaaagg ttctatggat tggctattaa aaaaaatgtt
     4981 ggaattttca taaaaaaaac tactccttac ttttatataa gtcaaaggag tagtttttaa
     5041 tttatacatc tagttttaaa tcttcaggtt ttattagacc tttttttctc ataaattctg
     5101 aaatcaacaa agttacaata gcaggaagta taaaattaag taaaagtata ccaacccaca
     5161 tagatatacc tccttttcca gcactttgca tagcagttac tgttccaaat tgtccaacca
     5221 atccacaagt acccatacca gaaccaatag gaatattctc cattttgaat attgttgtag
     5281 ataatggtcc aagtaatatt gaagctacag taggtggtat ccaaattttc caatttttta
     5341 ttatattagg tacttgcaac atagatgtcc caagaccttg tgccacaagg ccaccaactc
     5401 cattttctct aaagctcata acagcaaagc ctaccatttg gcaacaacaa cctacagttg
     5461 ctgcaccacc agcaagacca cctaaactta acatcataca aatagctgca ctacttatag
     5521 gtaatgtaag agctattcca acaagtgcag agactaaagc tcccattatt ataggttgca
     5581 tagttgtagc ttgcattatt atatttccaa atgaagtcat aaaagcagaa acagctggac
     5641 caataagttc agctacaagt actccaatta gtattgttac agcaggtgta accaatatat
     5701 ctatttttgt ttcttttgaa actaattttc caacctctac agcaaaaagt gtagcgacaa
     5761 atgctccaac aggtcctcct aattcattac cacaagctcc aacaagtgca caagaaaata
     5821 gtacaagtgg aggtgcttga agtccatacg caatagccac tgctatagct ggaccagtaa
     5881 cctgtttagc aagagggctt ataacttcag taaaaagaga aatattgaaa ttatctccta
     5941 atgtattgaa aattgttcca attaaaaggg acgaaaaaag accgagtgcc atataactta
     6001 gtgcgtcaat aagatacctt tgaacagaaa tttcaatatt ttttctttta gcaaatgatt
     6061 tgaaatttct ttttttataa ttgatttcca atttaaaatc ctcctcatat aatataagta
     6121 taatttcttt ttaattaatt atataacttt tcagctgaca agacaaatgt tatttcacta
     6181 aaattagact attgtaatag taagaaatta acattaattt tagttttaaa ttttaaattt
     6241 atggtttata attagtatag ggatattctg aaatataata attttccagt ttatcccaat
     6301 atttaactta ggtagattgc ttacaaaaca aatgattata tattaggcta aaaggaagtg
     6361 gatagatgat atttgtaaaa aataagaaat acagaaaaat tgccataatt atatctattc
     6421 ctatattgct tgttgcgcag tactaccttt ttaaatttgg aatacttaga ccaatggtaa
     6481 agggagtaga aattgaaatt gtagatgggg aatatgttaa agatatagat aaatatgtag
     6541 taaaattaaa tgaaacagtt actttgtcta caggtgaata tattaaggtt ccttcgtatg
     6601 ctaaaaatcc aaatatttgg tttaatgtat tggataactc agggacttta aaaatagatg
     6661 gaaataaaat gacagcatta aaggttgggt attcttctgt gggtatcatg aaaaattcaa
     6721 gagtattaaa aaaggctact ataaaagtag tagacccaga ggtagaatca ctagatatgg
     6781 agattaatgg ggatttaaaa catgttggtg atagtgctac tatagaaagt acaatagaag
     6841 ttgattatga taagtttaaa aaatcatata agccagttta taagagtagc aatgaaaata
     6901 ttttaaaaat caaaggaaat aaagtaagtg cagttggagt tggtaaagct acaatatatg
     6961 ctaagtgtgg aaataaagaa attgagagaa catttaggat tcaagctaga gttgctgaaa
     7021 taaatatgga tgatataaaa atagaagttg accagaatga aaaattgaaa ccagatataa
     7081 taacttctcc gcctggttta gaaccaccaa aaatatatta tgagttagta gattcaaaat
     7141 tacctgtaga aagagcaata agtttaagta gtaatggaaa tgttgtaggg cttagagaag
     7201 gtaaagatag ggtgagaata acttgtggag ataaatctaa agtaataaca gtaacagtag
     7261 tcaaagaaac tataacgaat gactatatac aaaacttggt tggttcacat aaaatagtaa
     7321 acaataaagt tattattaaa cttgaatggg attatatgaa agatgtcttt gattatgata
     7381 tatatcttaa agataagttg tcaggagaat catttaagaa aattaaatct ataactataa
     7441 atgaaagtga tttaggtaag tctaacaagg tatatgcaac tatagaagta gatatgaagg
     7501 gtaatcaaaa tatagatttt gatatttatg tcatagggaa aaaggattca gatacaacta
     7561 agccaagtaa tgtaataaat attaccaatg gaaatggaaa ttcaaataat gataataata
     7621 ttaaagatat gagggtagaa aatatatcag ctaatgttga tagagataat aatgtcatta
     7681 gagttaactg gagtagtata tctcataagg actgtactta cagtatatat gtaaaaaata
     7741 atactaatgg agatggagaa tttactctat atcaaagtga tatacaggga aatgagcata
     7801 ctataagtat tcctgaagga gatttaaatc tagatattta tgttgttgca aattataatg
     7861 gaaatagctc tcaaaatagt gatgttataa atgtaagata gatttgtata caaattgaaa
     7921 gtgttgagtt tatttaaata ttaaattaag aaaaaggata gtatagggta attatactat
     7981 ccttttttgt tacaatatat aggtttaaaa tctctagtaa tataaattat aaaaagtctt
     8041 atttacacaa atctatagat atttttttta ttaatcaata ttgtcttaaa ttgaacttag
     8101 tgcggcttct attgcatgta ttactgtcgt atcaaatagt ggaatagaag tgtcattttg
     8161 tttaattaaa agacctattt ctgtacatcc taatataaca gcctctgcac cttgtttgcc
     8221 aagcttatct ataatagata agaaagcttt ttttgaagat tcagatataa ttcctaagca
     8281 aagttcatta aatattgtgt catttactat tatacggtct tcttcatttg gaattaagac
     8341 ttcaattcca ttattaataa ttacattttt ataaaaatct tgttccattg tatatttagt
     8401 acctaataag gccacttttt ttatttcttt ttcccttaat accgtagcag ttacatctgc
     8461 tatgtgaagt aatggaatat gtatactttc ttgtatttta tctgatacct tatgcatagt
     8521 attagtacag ataacaataa aatctgcccc tgcctcttgt aatttaattg cagcatcagc
     8581 taaaattttg gcgctttttt cccaatttcc actagattgg cattcttcaa tttcttgaaa
     8641 atctacactg taaagtatac atttagaaga gtgtagtcca cctaatcttt cttttattac
     8701 tgtattaata acttgataat atgtaatggt actttcccaa ctcataccac caattaagcc
     8761 tatagtcttc atattatacc tctcttattt atttattttg aaatagctat ttacaaatac
     8821 aagtatagca tctaaaatag aagcatacaa tgataaaatt gtatggagta caattataat
     8881 taagaggagt aattatatgc caataaattc ttttgaaaac tatcctatga catggaaacc
     8941 aaaattagat aaccgaaaac caccaatata taaaacttta gcaatgttat tagaagaaga
     9001 tattaaaaga ggaaatttaa atcctggaga taaactgcct ccacaacggg aattagcaga
     9061 ttttctagat ttaaacttaa gtacaataac acgagcattt aaactttgcg aagaaaaggg
     9121 actaatatgt gcaaaagtgg gaaagggaac attcatttct tctgatgtaa atgtaagtaa
     9181 tacattatta tatcaaacgg aatcaaaaga cataattgaa ttagggacag ttcatcctcc
     9241 gtatgagcaa aatacatata ttattgactt tattaaaaat gtactaaaac aaccagaaat
     9301 ggataggttt ttacaataca tgtctccatc tggcacatat atgcaaaaaa agtcaattgc
     9361 aaaatggata gaaagaaata atgtatacac cagtgaagaa aatatattat tatctacagg
     9421 agggcaaaat gctatatgtg caactttact aggattattt aaagctggtg atagaattgc
     9481 tacagattct ttaagttttt ctggaattaa gtcaatagct aaaatgatag gtattcaatt
     9541 agtcccaatt cctcaagaaa ataatgaaat atcgattgaa tatcttgaaa gttattgtaa
     9601 aaatgaaaat ataaaaggca tatatcttat accagattat cataatccaa cgacacatac
     9661 tatgtctgat ttatctcgta aaaagatagc caagatagca aagcaatata atataataat
     9721 tattgaagat gctataaata gcatttttag agatggtata caaactccca tattttcttt
     9781 agctagtgat aatactatat atatttttag tacatctaaa tttttatgtg ctggtcttag
     9841 agtagcattt gtagttgctc caaaacgata tatagaaaat ttagaaaatg cactatataa
     9901 tatgaatcta atggtttctc catttacagc agaaatagta caccgtttat tatattctcc
     9961 tattatagat aaaattatag aagagaaaag agatgcaatt atagaaagaa atgaagttgc
    10021 tgataaagtt ttgtcagatt ataatttgat tggtgataag aattgcagtt ttcgttggtt
    10081 acttcttcct gatgaattgg atggaaaatc atttgaaatt tctgcaaaaa atctaggagt
    10141 tcaagtatat tgtgctgaac gattttcagt aggaaactct acagttccaa aagcagttcg
    10201 tatttgtgta acagctccaa aagatgtaga agaattagaa aaagggctta atataataaa
    10261 gtcacttctt aagagttaaa tttattttac tattatttaa ttaaataaat aaaaaggcgg
    10321 agaaatttat tataataaac ctccgctttt attatttaat gaaatacttt actttttatt
    10381 aaaaaattta attttaataa tcttgaaaat ataatcgttt tatggtaaaa taattcttaa
    10441 aaatagaaaa aattaatcat ttgttaaaaa aaatgcataa tttattgtaa tttattgacg
    10501 aaggttgaaa aatattcata aatgtgcgaa ataacagaaa aaatgcaaaa attaattcaa
    10561 tttcatttgt tataaaatct atatggcaaa attaaatctg cctatcagaa tgatataaac
    10621 acaccagaaa ggaggacgct atcaaataat acaagtagtt aactttgata gcaaaaaatt
    10681 atgaatgaac aaactagaat atccttagag agagctgctg aattaaaatc aaaaattgat
    10741 gattatattc aggctagaaa aacgattaac agaggtcttg aaaaagaaga agagataaat
    10801 aaacgaaaac agaaaatatt aagtatctta aatggaactg aagaggattg gaataactac
    10861 aaatggcaat tatcaaatag aataacagat gtagatactt tatcaaaaat tataactcta
    10921 actaaaaaag aaaaagaata tataaaagag gttggtactc aatttagatg ggcaatatct
    10981 ccatattatt tgagtcttat agacccagaa gatacatgtg acccaataaa attactgtct
    11041 ataccaacac atatagagtt ggaagatgaa caagaagatt tggacccaat gggagaagag
    11101 tatacaaacc cagcaggatg tataactaga agatacccgg atagattaat aataaatgta
    11161 acaaatgagt gtgctatgta ttgtagacac tgtcagagaa gaagaaatat tggacaacaa
    11221 gattctcata agtcaaaagc tattatccaa gaatctatag actatatcag agaaaatgaa
    11281 gaaataagag atgtactagt aactggtgga gatgctctta cattaaaaga tgattattta
    11341 gagtggattc ttagccaact taaagagata ccacatgttg attatgttag attaggtact
    11401 aggactcttg ttacaatgcc acaaagaatc acagatgaat tttgcaatat gctaaaaaaa
    11461 tatcaccctg tatatataaa tactcatttt aatcatccaa tggaaataac taaggaatct
    11521 aaagaagctt gtgaaaagtt agcaaatgca ggagttccat taggaaatca ggcagtatta
    11581 ttaaatggaa taaataatga taaatttgta atgagatgtt taaatcaaga attactgaaa
    11641 ataagagtaa aaccttatta tatattccaa agtaaacatg taaagggaac aaaacatttc
    11701 aatacatcag tagatgatgg tcttgaaatc atggagtatt taagaggata tacatcagga
    11761 atggctatac caacatatat agtaaatgct ccaaaaggag gcggaaagac tcctttgctt
    11821 cctcaatacc ttgtatcaaa aggaacagat tacgttatgc ttagaacatg ggaaggaaaa
    11881 gttataaaaa tggaagatga acctgctgta gatataaaga aacttataaa agaacaagcg
    11941 caggattaaa aaagtattta aagtttataa aaagaaacaa ctttaaaata aaaagaaaat
    12001 ggatgtcaaa tttatatcaa tttgcatcca ttttttatta gttatttatt attttgttgt
    12061 tgattaacta attcaagata tccacttggg tggaagtaat atatctcaga agtattttca
    12121 tttacaaagt aaccaatatc accatctgca tctggtaaaa atacataacc attaagattt
    12181 ttagctttta atgcttcaaa atcactagca tttccttgat atatgtaatc aacttcattg
    12241 ttgtatttta ctaaaagttc tttagcttgt tcttgagtca atttagtatt ttggcttaca
    12301 ggaacattct tttgagaatc tgcataagaa gattcattta ctggtgcagt taataacaaa
    12361 caagataaaa ctaataaaga tataagtgct tttttcatac taaaaccctc tttcatgttt
    12421 aattatataa gattcattac ttaataatat tgtatgtttc ctataattaa atattaccat
    12481 taaagagaat aatattacag ttacacagct gtaacttttg attacaaaat cgtaattaaa
    12541 ttgtattttt attccatttg tattttatca tgtctacgat ttacaaactc acagaagtat
    12601 atctttataa cgcccatcac aggagcagat agaatcatac caggtatacc aaagaatcct
    12661 ccaccaacag taactgctag gattgtaaag aatgggctaa gacctagttg tccacctacg
    12721 atttttggct ctataaatgt aacttcaact tgttgaatta ttattaagta aattaaagaa
    12781 gttaaagcaa ttgttggatt ggagaataaa ttaattatta caactggtgc catacctatt
    12841 actggaccaa aatatggaac catattcatg acacccataa gtgtaccaaa taatagtgca
    12901 tattcagatt ttaaaaagta aagaccaatt ccagaaagta ctccaactat aaaagaatct
    12961 aaaatcttac ctgtaaagta tttaccaata tttaaattta agctttgaca tagttctatt
    13021 ataaagtctc catatttttt acctaaggaa atataaacta ctttttttgt aaacagtaag
    13081 aatttttctt tttctaataa tatatagaaa caaattataa aacctaaaag aaattgtact
    13141 ataaattttc caactgaaaa tgtagtgcta aatatcccac caagtgaacc aataagcaag
    13201 ttactaaatt taggcattac actcataatt ttgtcaccat actcttttaa tgtattaggg
    13261 tcaactgatt gtaaactctt gcctaagtcg aataaaaact gctctgtttt atctatgtat
    13321 gtaggaattt gagcaactaa atcagctaaa ctatttacta tggatggaac tgtaaataat
    13381 ataaacgaaa ttagtataac tatgagtaca ccataagtaa gaagtaacga atatattctc
    13441 ttaaaattta atttactttc taagaacttt actagtgggt tgaatatata tgctaataca
    13501 aatgctatta taaatggtgt tagtagtgac attaataaac ttatcactcc aaaaaaatat
    13561 ttgtaattat caataacttt aatcaagata tatgaaacaa ctacagtgag tacaatattt
    13621 agataaattt ttttatcttg caaaataaca caacctttct tatatttatt aaatttagat
    13681 atacttaaat ctaagttaaa gttaatattt atagcttatt atacaataaa aagcagtatt
    13741 aatatagtat gattaatatg aaat
//
LOCUS       DAYATN010000104        13687 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.104, whole genome shotgun sequence.
ACCESSION   DAYATN010000104 DAYATN010000000
VERSION     DAYATN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.104"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..421
                     /locus_tag="ACGI8A_002747"
     CDS             <1..421
                     /locus_tag="ACGI8A_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180737.1"
                     /translation="IKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLV
                     ENELRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEK
                     IEINKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            674..1711
                     /locus_tag="ACGI8A_002748"
     CDS             674..1711
                     /locus_tag="ACGI8A_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HFH0180738.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLNGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            1792..2157
                     /locus_tag="ACGI8A_002749"
     CDS             1792..2157
                     /locus_tag="ACGI8A_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180739.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            2162..2479
                     /locus_tag="ACGI8A_002750"
     CDS             2162..2479
                     /locus_tag="ACGI8A_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180740.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            2527..4131
                     /locus_tag="ACGI8A_002751"
     CDS             2527..4131
                     /locus_tag="ACGI8A_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFH0180741.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            4255..5883
                     /locus_tag="ACGI8A_002752"
     CDS             4255..5883
                     /locus_tag="ACGI8A_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFH0180742.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            6148..7497
                     /locus_tag="ACGI8A_002753"
     CDS             6148..7497
                     /locus_tag="ACGI8A_002753"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFH0180743.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            7653..8732
                     /locus_tag="ACGI8A_002754"
     CDS             7653..8732
                     /locus_tag="ACGI8A_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFH0180744.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            9077..9985
                     /locus_tag="ACGI8A_002755"
     CDS             9077..9985
                     /locus_tag="ACGI8A_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase activase"
                     /protein_id="HFH0180745.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            9990..12359
                     /gene="hypD"
                     /locus_tag="ACGI8A_002756"
     CDS             9990..12359
                     /gene="hypD"
                     /locus_tag="ACGI8A_002756"
                     /EC_number="4.2.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase"
                     /protein_id="HFH0180746.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            12684..13487
                     /gene="proC"
                     /locus_tag="ACGI8A_002757"
     CDS             12684..13487
                     /gene="proC"
                     /locus_tag="ACGI8A_002757"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFH0180747.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEKFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         5565 a         1429 c         2540 g         4153 t
ORIGIN      
        1 aataaaggat aataagcata tagatgataa atatatagaa agtgttgaat taaataaaaa
       61 tgaagaattg aaaaataatt ttaataactc atttttagat aaaaaaacaa ataaagatat
      121 agtgttatta gtagaaaatg aactgagaga tattgtgaat gtagaatata ttaatcaaga
      181 aagtagttta gataatcaca ataaaaaaca agttaattta gaaataaaag ttaatggaga
      241 acttgcaaat gtgttgaaac ttgaaggtgt aatgaagaag ttaagactaa ataataaact
      301 tgaaaagata gaaattaaca aattaaaagt caataatttt gatgttcaaa atattccaaa
      361 tgaatataaa caagaaaaag acaatattgt tgaatgtaaa atgacagtta gagtcatata
      421 acaaaaaaga atagataaag taaaaaacta ttatcaatta ataaaatttt atagaggaat
      481 tagttttacg ataattcatt tctacaagag ggattgttga tgattttatc gaaatcctaa
      541 ttaaagttaa tatattgtac caaaatataa gtgaatttaa tagaatattt acttatacta
      601 tagattgatt aataaaaaaa tggtataata acaaattgta ctttaattta ataataaggg
      661 agtggaagtc atattgttag ggagaaatga gctatgccct tgtggaagtg gcaaaaaata
      721 caagaaatgt tgtttaaata aagatattgt ttcagaaaga gcatgcagaa aaactgaact
      781 ttcacaaaaa caatatacag atttatattc aaagttatat gattattcaa gacaggataa
      841 gtttaaagaa gaatgtgaaa aagctaaaga aatgttttat ataatgcaaa atgatagtgt
      901 aaatgaaaaa tttgaaagat tttttaatac ttatttaata caagaccaca taatggaaaa
      961 taaaaaggtt atgacagttg gttttcttga agaaaatggt gcaaacctaa gtcaatcaga
     1021 agtatcaata ttaaaaagta tgtttgaatc atacgtaagt atttatgagg taaaagaagt
     1081 ttctgctgga aaaatagtat taaaggactg tttaaatgga aatgaacttt gtacagaaga
     1141 tgtaaaactt ctgagaagtt ttaaaatcgg aagttgtatg atagctagaa tagttgatat
     1201 tgaaggaact aacatattga tagatataac tataagtata tcaaatgagg ttaaagatat
     1261 aattttaaag gatattatga atctattcaa tcaatatcaa gatgtataca aagatatgaa
     1321 gtcgttctta atttatcata cacatatact atacaagtat atacaacagc ttttagaccc
     1381 aagtatagct gaatatttaa agagagaaag agataaaaac gatacaaaag ttaattcaga
     1441 aaatgaatta gctgaagaag aatgtaaagt tttagatact cttaaagaaa atgtagaaaa
     1501 agaaaatata gatgcatgta tagagttttg gaatgaatat aagaaaaatc atgaaaatat
     1561 aaaaggagct gaaaatggtt gggcagctgc tgttgaatac tatgttaaaa aagaagcagg
     1621 acaacctata actcaagttc aaatatcaaa gaaatacgat ataagctcta gtacactagg
     1681 aaagagatac aaggatttaa aaatatctta attaattata aaaagcttta agtcatagat
     1741 aaagaatcta tagtataata tatgtataat gtttagaaag aggtattaat aatgagtctt
     1801 agtttaggaa atgcaattgc tgtatcaacg aactttataa aatctaaccc agctgtacct
     1861 gtgtacaacg gagaagaaat atatgcagtt atttatcata tagaaatagg atataataat
     1921 ggtgtatcac ctgataccaa acaatatata ataatagact ataaagagtt tgatttatct
     1981 atggataaaa aaaatgaact aataaatatt gctaaagagt actgtaaatc taaaggtatt
     2041 gtagaaaacg aaaaccttga aataaacctt ttagacaatg aagaagcaga gaacttctta
     2101 gaaaaggtgt ttaataatat gaaggttata agaggtctta taactaaaaa aggataaata
     2161 aatggactat ataggaatag aaaatataac accttatgaa aatacatatg aatttagtgt
     2221 atatgaatat gatgatgaaa tcaccttagg tagtgaaaag ttatatgtat gtgaattaag
     2281 ggttgtattg attaaagtta attctctgta tgttgaaaga ttgcataaat cagttgaagc
     2341 aatggtctta gtaaaaaact tgaaaaaaga tttagataaa acacttgttg taaacaaaat
     2401 aaagaatttt gtgctagatg agatttgggt agaaaatcta gtaaaagaga atatagaagt
     2461 tatatttgta gaaagctagg aataacccta gcttttttat attacaatct aagagaggaa
     2521 aacaatatgt taagagtatc taatgtaaaa cttggaattg ataaagatat atctttatta
     2581 aaagatgtta tacttaagaa gctaaaaata aaagaaaaaa acttagtcaa atatagtata
     2641 tataaagaat ctatagatgc tagaaaaaaa ggaaaaatgg aatttgttta ttctgtagat
     2701 gtagaagtta aagatgaaaa taaaatactt aactcaaaaa taaaagatgt aacaaaaata
     2761 aaggaaatca gatacgtaaa tgtacctgtg ggtgataaaa aattaaagaa taatcctttg
     2821 gtaattggta gtggaccagc tggcttgttt gcaggtctgc ttttagctca aatgggttat
     2881 gaaccaatca tacttgaaag aggtcttgat gtagacagta gaaccaaaga tataagtgat
     2941 ttttggacaa ctagaaaatt aaaaaataat tcaaacgttc aatttggaga aggtggagca
     3001 ggaacttttt cggatggtaa acttacaact agaataaaag atatacgatg tagaaaagta
     3061 ttagaagagt tagttcagtt tggagcacca gatgaaatat tatactcaca taagccacat
     3121 gttggaacag atatattaaa aaatgtagtt aaaaatataa gaaatgaaat aataagttta
     3181 ggtggtcaag ttagatttaa ttcaaaagta acagatatag ttgtatcaaa tgattctata
     3241 caatctgtaa taataaatga tactgaaaaa atagatacag atacaattat attagctgtt
     3301 ggacatagtg caagagatac ttatgaaatg ttgtattcaa gaggtataaa aatagttcaa
     3361 aaaccttttg caataggagc tagagttgaa catccacaag atttaataaa taaagcacag
     3421 tacaaggatt tttataatca tcctaggtta ggagctgcag attatagact tatagagcat
     3481 gtttcaaatg gaagaacagc atatactttt tgtatgtgtc caggaggtag tgtcatagca
     3541 tctgcatcaa atgattttga aatagttaca aatggtatga gtgaacatgc aagagataaa
     3601 ataaatgcaa atagtgcatt tttagtaaat gtaacgccag aagattttaa gagtgataat
     3661 ccactagcag gtgttcattt ccagcagaag tatgaaaaat tagcttttga attaggtgga
     3721 aaaaattata atgcaccagt tcagcttgtt ggagatttta taaacaataa gacttctttg
     3781 aatttaggaa gtgttgagcc atcatataga ccaggatata cacttacaaa tttaagtgaa
     3841 tgtttgccta gcttcgtaac agagactatg agagaagcgt ttatatctct tgataaaaaa
     3901 ttgaatgggt ttgcaatgta tgatgctgtt ttgacgggag tagaaactag gtcatcatca
     3961 ccaataagaa tagtaagaga tgaaaatact ttagaatcag tgagtattaa gaatctatat
     4021 ccatgtggtg aaggtgctgg atatgcaggt ggtatagtta cagcagcagt tgatggtata
     4081 aaatgtgcag aaaaaataat acaaaaatac accaataata caaaaattta acataaactt
     4141 aatgattttt taatatttaa catgaattta acattatcac tcctataatt tgataaaata
     4201 caataggtta taaaattttt agatacagta atctctttac aaggaggaaa taatttgaac
     4261 attataataa gtctaatggg tggattaggt ctgttcttat atggtatgaa ccttatggga
     4321 gaaggtcttc aaaaatctgc tgggacaaag ctaaaaaaga taataaaact acttactagt
     4381 aacctattta tgggagtttt ggtaggtaca ggagttacag cagtaataca aagttcaagt
     4441 gctactactg taatggttgt tggtttcgta aatgcaggaa taatgacttt aaaacaggca
     4501 ataggagtta taatgggtgc taatataggg actactgtta cagcccaatt ggtatctttc
     4561 gacttaactg gtatggcccc agtagcatta ggtgtaggta ttatacttta tttatttgga
     4621 aataagccta gaataaaaaa tatagccgaa atacttatag gttttggtat attatttaca
     4681 ggtatggatt ttatgaagat ggcagtggag ccactaagag actatcaagg atttactgac
     4741 ttacttgtta catttggtag atatccacta cttggattat tattgggatt tgggattaca
     4801 gcaataatac aaagttcaag tgcatctatg ggtatgttgg ttgcactggc agcagaaggt
     4861 ctagtgccat tatcagcagc actgccaatt ctttatggcc aaaatattgg tacatgtgta
     4921 acttcactgt tatcaagtat tggtgctaac aaaaatgcac gaagagcagc tatgatgcat
     4981 ctaatcttca atgttttagg aacggtgata ttcttaatat tcttgaataa accagtagta
     5041 agcatggtta caagttggga cccaagcaat gttgcaagac aaatagccaa tacacataca
     5101 ttgtttaata taatatctgt attgatactt ttaccattta caaatcttat tataaaacta
     5161 gctataaagc ttgttcctga tagagcaggt gatatagatg aagatgaaac taaaactatt
     5221 aaatatatag atgatagaat gatagaaact ccttctatag ctcttgcaaa tacgataaaa
     5281 gaagcacttc gtatgggtga gaaggcgaag gaaagcctaa atgcatctat ggaagcttta
     5341 gtagaacatt caactgaaaa aatagataag acatataggc gagagagatt aatcaatgat
     5401 ttacagaaag ctattttaaa ttatttactt aaactttcaa aagcaccttt agatgatgat
     5461 tcaagagaag ttgtggatac tttgtttaat acagtcaatg atatagaaag aattggtgac
     5521 cacgctaaaa atatagctga attatcgcaa gttgcaatag attcaaatat ttctttctca
     5581 gaagaaggac aatctgaact tgatgttatg tataatagag ttgtttcagc atatacttat
     5641 gcattagaat caatgagaac tgataatgtt gatttagctt gtaaggttat aaaaattgaa
     5701 gaacaagttg atataatgga gaaatcttgt agagctaatc acatgtatag attgaacaat
     5761 aatctatgta gtatagaaaa tggagttatt tatttagacg taataagtaa tttagagaga
     5821 atatctgacc atgctgtcaa tatagcacaa caagttatag caaagagatt aggaaatgat
     5881 taaaaataat taatttaaaa taatataaaa tttaaaatag aggataaata aaagagaaac
     5941 tttaaatgat acgtttagag tttctttttt tgtaaattta tttgactaaa atttttaaat
     6001 ttatacatag ttatagcatt tagaagaatc gatttcctga taataaatga tgagatatgt
     6061 atgattttgt ggcaaacttt taaaaaaaac acctatattt tgtgttataa tctctttaaa
     6121 acgaatatgc tgaaaagagg gatttaaatg ggaaaaataa attttgatta tagtaaagct
     6181 acagagtttt tttgtcaaaa cgaaatagat gtcatgcaac catatgtaga tgttgcgcac
     6241 gacatgttac ataataaaac tggtttagga aatacttttt taggatggat agaccttcct
     6301 aaaaattatg ataaagaaga gtttgacaga ataaaaaaat ctgctgataa gataaaatca
     6361 gactcagatg ttcttttggt aataggtata ggaggctctt atttaggttc gagagctgct
     6421 atagatatgg taagtcattc ttttaggaat ggacttaaaa aagagcaaag aaaggctcca
     6481 gaagtatatt ttgtagggca taatataagc tcaacatata ttatggattt attagatata
     6541 attgaaggaa aggatatttc tgtaaatgta atatctaaat caggaactac tacagaacca
     6601 gctctggctt ttagaatatt taaagattac ttagagaaaa agtatggtaa ggaagaagca
     6661 agaaagagaa tttatgcaac aacagatgca agtaaaggtg cacttagaca gttagctaca
     6721 gaagaaggat atgagacttt tgtaatacca gatgatgtag gtggtcgttt ttctgtattg
     6781 acagcagttg ggttacttcc tattgctgct gctggacttg atattgatgc tatgatgaaa
     6841 ggcgcaaatg atgctagaga agcatttcaa aatcctgact taaaaagtaa tgatagttat
     6901 agatatgctg ttgctagaac aattttacat agaaaaggaa aagatgtaga gttattagtg
     6961 aactatgaac ctcaactaca ttatgtaagt gagtggtgga aacagcttta tggagaaagt
     7021 gaaggaaaag aaaataaagg tttattccct gcttctgtag atttctcaac agatttacat
     7081 tctatgggtc aatacataca agatggaaaa aggcttttat ttgagacagt acttaatgtt
     7141 gaaaactgta agagaaatat aactataagt tcagaggaag tagacttaga tggtttaaac
     7201 tatttagcag gtaagactgt agattttgta aatcataaag cttttgaagg aactctattg
     7261 gctcatacag atggaaaagt acctaattta gtaataaata tacctcaact agatgagtat
     7321 aattttggat atttagttta tttctttgag aaggcttgtg gaataagtgg atatttattg
     7381 ggagttaatc catttgacca accaggagta gaagcttaca agaaaaatat gtttgcttta
     7441 ctaggcaaac caggttatga aaaagaaaag gaagagctag aaaaaagact taaataattt
     7501 taaatagtta aggtttaatt aagattaaaa taatataatg aaatcatcga aaaggagttt
     7561 actcaaaata gatgatttca ttttttttat gaaaaattgg gaaaaatata ttttgataat
     7621 ttatattata ttaagatagg gagtggtatg agatgtcaag aagaaagaaa gggataagtt
     7681 tagtaatatt agttgctata ataagttcaa tacttagtag ttttctaaca ataattttag
     7741 ttaaggacaa tttagtaagt aaatctacag gaagttctac gccgatagtt gtaaatgatg
     7801 atggtaagag tcaaaatatt tatcaagcag ttgcagaaaa agctacgcca tctgttgtag
     7861 gtattacaac aactagtgtt gatactagta atatgtttgc aataccaact gaaacacaag
     7921 gtgtaggaac tggaatcata gttgattcaa atggatatat acttactaat tctcatgtta
     7981 tatcagatgg tcaagctaca agtgtaaatg tattattcaa tgatggttca acaacttcag
     8041 gtaaagtagt atggtttgac caacaacttg atttagctat agttaaggtt gataaaactg
     8101 gactaacacc agcagaattt gctgatagtg acaaagtaaa agttggagat atatcaatag
     8161 caataggtaa tcctcttgga ttagattttc aaaaaacagt tactcaaggg ataataagtg
     8221 gattagatag aactatacaa acagaaaaaa ctaatatgac tggtttatta caaactgatg
     8281 caagtataaa tgcaggaaat agtggaggac cattgttaaa tcaaaaaggt caagtaattg
     8341 gtataaacac agcaaaagct tctcaagcag aaggattagg atttgcaata ccaatcaata
     8401 cagctaagag tatagttgaa gaagtaatca aaaatggaaa atatgaaaaa gtaactttag
     8461 gcataaaagg tacagatgta tcaaattatg aagcagcaac aggtacaaaa ttaagtactg
     8521 ataaaggtgt ttatgttgca gaagtaatat ctggttcatc agcagaaaaa gctggagtaa
     8581 aggtagggga tattataact aaagtaggag atacagatat aacaggtatg aatgatttaa
     8641 ataaaaagtt gtatactttc tcaaaaggtg ctagtacaaa aataacagta aatagaggcg
     8701 gaaaagcagt aacaataaat gtgaatttct aaatatgacc agaaagatag agactacatg
     8761 gtaaagtgta gtctctattt ttatttaatt tttatagtat atacatctaa attaattaaa
     8821 atgatgtatt gtttatagtt tattgctata ttttcttata aaaataaatt taataaatat
     8881 ttaaatttaa tttacaaaaa caatataaat atcataataa aataaaattt tttaatatta
     8941 ctaaaaatat atagacaaaa taataacgaa tatagtatta tgtaattgta gaaatgcttt
     9001 tcataggtat attctatgaa gatgaattgc ataaaaatga aaaaatggaa tggatattat
     9061 ttaggggtga aaaatcatga atccattagt tataaactta caaaaatgta gcatacatga
     9121 tggaccaggg ataagaagta cagtattttt taaagggtgt ccacttgaat gtgtttggtg
     9181 tcataatcca gagagtcaaa catatacaaa acaagttttg tataatgaag aaagatgttc
     9241 aaaatgtgaa gcatgtataa atatatgccc tcataaggca atttacaaag gtgaaacaaa
     9301 aatatgctta gaccaagata agtgtgagtt ttgtgaaact tgcttagact attgtgtaaa
     9361 caatgcaaga gagattgttg gtcaagagta ttctgtaaga gatttggtta aagagataga
     9421 gaaggataga attttctatg aagaatcagg tggtggagta actctatcag gtggagaagt
     9481 aatggctcaa gatatggact tcatttgtgg agtaataaac atgtgcaaat caaagggaat
     9541 ccatgtagca attgatacat gtggctatgc aaagtcagaa aattatgaaa gagttgctaa
     9601 atgtgcagat ttgtttttat atgatattaa gcttattgat gaagataagc acataaaatt
     9661 tacaggaaaa tcaaatgact taatactgaa aaatgttaag attttaagtg aattaggggc
     9721 taacataaat ataagaatac cattaattgt aggagtaaat gtagatgatg aaaatttaga
     9781 ggttaaaaaa atgatagagt ttttaaagcc tttgaatata caagcagtaa gtcttcttcc
     9841 atatcataat attggtaaac acaagtatga taaaatttat aaaaagtatg aaggagaaga
     9901 actacaaaga ccatcagaag aaaaattaga ggaaataaaa agactgtttg aagcaagtaa
     9961 tttcaatact aagattggag ggtaataaaa tggcaagagg aacttttgag agaactaaaa
    10021 aattaagaga agaaagtatt aatgcagaac cacacatatc tattgaaaga gcagtactta
    10081 tgacagaagc ttataaaaaa tatgagggaa gtgtagagat tcctgtactt cgtgctttat
    10141 catttaagca ttatattgag aatagaactc ttagcataaa tgatggagaa ttaatagtag
    10201 gtgaaaaagg tgattctcct aatggagcac caacatatcc agaaatttgt tgtcatacaa
    10261 tggaagattt agaagtaatg cacaatagag atataataaa ttttagcgta tcagaagaag
    10321 ctagaaaaat acataaagaa gaaataattc ctttttggaa aaagagacaa acaagagata
    10381 agataataaa tgcaatgaca cctgagtggt tagcagcata tgaagcaggt atgtttacag
    10441 agtttatgga acaaagagct ccaggacata cagtttgtgg agatacaata tataaaaaag
    10501 gatttttaga tttaaagaaa gacatagaag caagattaaa agagctagat tttcttaatg
    10561 atttagatgc atataataaa aaagcagatt tagaagctat ggcaatagct tgtgatgcaa
    10621 tggttatatt aggtaaaaga tatgctgaga aagcaagaca aatggcagaa gaagaaacag
    10681 atgaagctaa gaaaaaagat ttattactaa ttgctgaaac ttgtgatgtt gtaccagctc
    10741 acaaaccaga aacttatcat caagctatac aaatgtattg gtttgttcat ataggggtta
    10801 ctactgaact taatatatgg gatgctttta ctccaggaag acttgaccaa catctaaatc
    10861 ctttctatga aagagatgta gaaaatggaa tactagatag agatagagca caagaattac
    10921 ttgaatgttt atgggttaaa tttaacaatc agccagcacc accaaaagtt ggtataacat
    10981 taaaagaaag tagcacatat acagattttg caaatataaa cactggtgga ataaatccag
    11041 atggtcaaga tggtgttaat gaagttagtt atataatact tgatgttatg gatgaaatga
    11101 aattgataca acctagttca aatgttcaaa taagtaaaaa gactcctcaa aaattcttaa
    11161 agagagcttg tgaaatatca agaaaaggat ggggtcaacc agcattttat aatactgaag
    11221 caatagttca agagcttatg gaagctggaa aaactataga agatgcaaga cttggtggaa
    11281 ccagtggttg tgttgaaact ggatgttttg gtaaagaagc atatgttcta actggatata
    11341 tgaacattcc aaaaatactt gaattaacat taaataatgg atatgaccca attagtaaaa
    11401 aacaaatagg tattgaaact ggagacccta gaaatttcca atcttatgag gaactatttg
    11461 aagcatttaa aaagcaatta cattatatga ttgacataaa aatagaagga aatgctgtga
    11521 tagaaaatat atgtgcaaaa cacatgcctt gtccacttat gtctacaata gttgatgatt
    11581 gtatagaaaa aggcaaggat taccaaagag gtggagcaag atacaacact agatatatac
    11641 aaggtgttgg tataggaact ataacagata gtttaacagc aataaaatat aatgtatttg
    11701 ataagaaaaa attcgatatg gatacattgc ttaaagcact tgatgctaat tttgaaggat
    11761 atgaagctat acttaattta gtttcaaata agactcctaa atatggaaat gatgatgatt
    11821 atgctgatga aataatgcaa gagatattca atgcatatta caatgaggta actggtagac
    11881 caactgtttg tggtggagaa tacagagtag atatgttacc aacaacttgt catatatact
    11941 ttggagaaat tatgggagca agtccaaatg gtagactttg tgcaaaacca gtttcagagg
    12001 gaatttcacc tgaaaaaggt ggagatacta atggacctac agcagttata aaatcttgtg
    12061 ctaagatgga ccacataaaa actggtggaa cattattaaa tcaaagattt gcaccatcag
    12121 tagttcaagg agaaaaaggg ttagataata tggctaatct agtaagagca tactttaata
    12181 tggatggtca tcatatacaa tttaatgtat ttgataaaaa tgtactgtta gaggctcaaa
    12241 agaatcctca ggattataaa gacttaatag ttagagttgc aggatatagt gaccatttca
    12301 ataatttaag tagaacacta caagatgaga taataggaag aactgagcaa acattctagg
    12361 tttgcttgta aataaaactt cttactatat aaaatcgtaa ctgatataat aaagtataaa
    12421 tggtttgatt ccatttttaa aattttagat taattatgag ctaactttct aaataggaag
    12481 ttagctcgtt aagtttaatg aaaaaatcgt taaatttata tttagtaaat catacacata
    12541 ttagtaagta atacatacaa aagaataatt tattatagtt ataataaggt tatattttat
    12601 aaaaataaga taattttaag cagttaaaat aaagtcaatt tagctacttt aaaataaaac
    12661 taatagaaaa aaggagattt gatatgaaaa ctttaggatt tattggttca gggaatatgg
    12721 gaagtgcaat gataggtggc atagtacagt caggattagt aaaggcagaa gatataactg
    12781 tatcagattt aagccaagtt gccttagata aagtaaaaga aaaatttgga gtaaatacta
    12841 caactgattc aaaggtagct gttacaaatt ctgatgtggt aatagtagca cttaaaccta
    12901 atatatgcga aaaggcttta actccactta aagaattaat agatgcaaat aaaataatag
    12961 tatctatagc agcaggtaaa actatagaaa gtctggaaag ttttataggt tcagataaaa
    13021 aaatagtaag agtaatgcct aatactccag cccttgttgg cgaaggaatg tcagctcttt
    13081 gtaaaaatag taatgtaaca gatgaagagt taaatatgat taaggctctg tttgaatctt
    13141 ttggtgaggc agaaatagta tcagaatatt taatggatac agttacaggt attagtggtt
    13201 cttctcctgc atatgtattt atgtttatag aagctatggc agatgcagca gtattagctg
    13261 gaatgccaag acaacaagct tataaatttg catctcaagc agtaatgggg tcagctaaga
    13321 tggttcttga aactggaaaa catccaggtg aattaaaaga tatggtttgt tctccagcag
    13381 gaactactat agaagctgtt aaggtattag aagaagaagg ctttagagca gcagttataa
    13441 aatctattgt tgcttgtata gataagtcta aagatatgag caaataacag atgtaattaa
    13501 gtttactata tggtttcttg aattgttttt ttagtgctta tttatatcta tacataaatg
    13561 tatttggtat aagtaaaata agagtaattt actataaaaa ggaatttaat gattgagtga
    13621 taatttatta cttaaatatt aataattaaa tagatatatt tttaactata tttgagatat
    13681 atcattg
//
LOCUS       DAYATN010000105        13660 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.105, whole genome shotgun sequence.
ACCESSION   DAYATN010000105 DAYATN010000000
VERSION     DAYATN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.105"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(396..2063)
                     /locus_tag="ACGI8A_002758"
     CDS             complement(396..2063)
                     /locus_tag="ACGI8A_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HFH0180748.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(2137..2892)
                     /locus_tag="ACGI8A_002759"
     CDS             complement(2137..2892)
                     /locus_tag="ACGI8A_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFH0180749.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EILTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(2902..3438)
                     /gene="lepB"
                     /locus_tag="ACGI8A_002760"
     CDS             complement(2902..3438)
                     /gene="lepB"
                     /locus_tag="ACGI8A_002760"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFH0180750.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(3604..4140)
                     /gene="lepB"
                     /locus_tag="ACGI8A_002761"
     CDS             complement(3604..4140)
                     /gene="lepB"
                     /locus_tag="ACGI8A_002761"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFH0180751.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(4240..5550)
                     /locus_tag="ACGI8A_002762"
     CDS             complement(4240..5550)
                     /locus_tag="ACGI8A_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFH0180752.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(5629..6345)
                     /locus_tag="ACGI8A_002763"
     CDS             complement(5629..6345)
                     /locus_tag="ACGI8A_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HFH0180753.1"
                     /translation="MKLDLFLLAIIPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            6590..7861
                     /gene="sleC"
                     /locus_tag="ACGI8A_002764"
     CDS             6590..7861
                     /gene="sleC"
                     /locus_tag="ACGI8A_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic germination protein SleC"
                     /protein_id="HFH0180754.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(8287..8943)
                     /locus_tag="ACGI8A_002765"
     CDS             complement(8287..8943)
                     /locus_tag="ACGI8A_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180755.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGVRILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(10055..10465)
                     /locus_tag="ACGI8A_002766"
     CDS             complement(10055..10465)
                     /locus_tag="ACGI8A_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180756.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(10681..12063)
                     /locus_tag="ACGI8A_002767"
     CDS             complement(10681..12063)
                     /locus_tag="ACGI8A_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFH0180757.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVILRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(12074..12433)
                     /locus_tag="ACGI8A_002768"
     CDS             complement(12074..12433)
                     /locus_tag="ACGI8A_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0180758.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(12638..13417)
                     /locus_tag="ACGI8A_002769"
     CDS             complement(12638..13417)
                     /locus_tag="ACGI8A_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFH0180759.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         4660 a         2466 c         1334 g         5200 t
ORIGIN      
        1 aataattcaa taatttaaca acctagtaat ttagcaatcc agcaatctaa taatccagta
       61 atttaaattt ttataacacc aaaaatttct aacccaacac cttttactct tttactcttt
      121 tactctttta ctcttttact cttttactct tttactcttt tactctttta aaaaataatc
      181 acataaatta acaaaatatt aaacaaaaaa gtaaaattta aaataaaaag cttgcttcat
      241 acaaaagtat aaaacaagcc tttttatata tcaactcaaa ctttaaaatc tgtatcaatt
      301 taattctcca ctttacactt aatatactta acataaacca ataacactac ttgctatgcc
      361 atttaatctt cataaaacaa tctataatac acacattaat caactatttt acttcctcca
      421 ataaattccc taagaattga aatacctggt atgtcagaag tatcgtccaa ttcaacaaaa
      481 tactgcaaaa actttagtaa tctagtagct tcaatatgtg caggattatc ttcctttatc
      541 tcttccaaca aaggtttcgc aaatcgtttt aaaactgcca attcatatac acaagcagag
      601 ttaaatatag catctgcctc ctcttggtac gggaatatat tcttcttttc tcctcttcta
      661 actgatgccc attgtaaaat agtattatca gcactatatc ctctaaaatt atagtctcta
      721 acaattcttc ttatcattct caaatcagca gtagggattc gattatggtc atccaaattt
      781 atttgagtaa gggcacttat gtatatctta aatttatctt catctggtat agatgatgtt
      841 aaaataggat ttagcccatg tataccttct aatataattg gctgatttga atctatcttt
      901 atctttttac cagtgtattc tctcttacct tctttaaaat tgaatcttat atcatcaatt
      961 tcttcaccag ctaaaagttt ctttaagtct aagttaaact tctctaaatc tattgcataa
     1021 attgattcaa aatcataatt tccatattca tctaaaggag tatgttttct atctataaaa
     1081 taatcatcta aagaaattga aataggattt aaattattta ctctaagatg tattgataat
     1141 ctgtgagcaa aacttgtttt tccagatgaa gatggagctg ctattaaaac tactctctta
     1201 ttgccatcct ttatcatatc cgctatttgg gataacttct tttcatgcaa tgcttcaaat
     1261 gtccttatta tatctccata ttcattgttt tctattattt tattaagaga tactaccttg
     1321 tcaattccca taagtcttga ccattcttct gcttcataat atacacttga tagcttaggt
     1381 tgaggtacaa atttcatagg aagagtctta tcttcttcac taggtcctaa aattataacg
     1441 cctccattat aaagcttaat atcaaatgat tttatataac cagttgatgg caacatataa
     1501 ccataaaaat ggtctatata gtttctacac ttatatactt tagcactatc atactctttg
     1561 tatttcaaaa gctctgcctt ttcatacatt tcattatttt caaagataga aatagcttca
     1621 tttcttgtaa cttcaatttt ttctataaca taatcgttgt ttactatctc tttcatcttg
     1681 ttttttatat tttcaacatc tgcttcttta agctttctat ctatatgaac ttcacaatat
     1741 agcccatctg atagtgaatg ttctattgta actctgctat cccaaaagat ttctctacat
     1801 gccattacaa atataaatga catgactcta aagtaaactc tctctccatc ttcattagta
     1861 gtatctaaaa aatttatctc acaatccttc ttaactctac aatttagctc ttttagttta
     1921 ttgtctacaa ctgcaagtgt tatataaccc ttatgctcat cacaaaattc ttttgctata
     1981 tctcccaatc tgattccagg ggaattttct tctaatgaat attccttatc ttttccttct
     2041 atttttaagt ttattttttt cacttcaaca tctccttcct tttgatgaat ataaatttta
     2101 attaatgagg catatcaaca agataaagag aggtatttaa ccctctccta caatattatt
     2161 caatttactt atagtatctt caatgtcttc gtaatttatt tctaatacta aatattctgc
     2221 atctaatgtt aagatctctt taatcccctc ttcagataaa attcctcttc ctataccaac
     2281 atgtgtatca ttgataccat cattatcact taagtgtatc agtcttattt tatcctttaa
     2341 gttctttaca taagcatcct catcaattaa acagtgtcca gtatcgtaac aaaaacaaat
     2401 cttagaattt ttaatcctct taaatatata ttcaaaatca taagatatat ttccaatatt
     2461 tgaaaaatcc ccaccttttg aataagtatt ctctatacat acatatgaat ttgtatttaa
     2521 tttatctaaa aaatccacac tatttcctaa atatttgtct ctattctttc taactctatt
     2581 tgtcataaca tatcccaaat gaaggttaaa ataccttaaa tcaaatcctt ttagttcaaa
     2641 ttctatcttc tcaataaaat ttatccatga atttcgtata tactctatgt tttcgcaagt
     2701 atttaattcc ataggcaaat gtatccctat agataatcct aacttagaaa ttctctcttt
     2761 atatttacat aactcactac attcagatat attgtcaatt ccaatctcta aatgagttat
     2821 ttgctttata ctctcacaaa tgtttaaagc ctcctcaata ttaaacaata aggacgctat
     2881 accaactgcc atacaaatca cctactcaaa aatacctata tctgtaaaag gaaatactct
     2941 taaaataact tttcctttaa tattttcttc atctactaat cccacttctt tatatctact
     3001 gtctaaacta acttctctat tgtctcccat cgcaaatact tttcccttag gaatcaccat
     3061 atcaatatcg ccttcagtat aattatcatg tatatatgaa acttcattta ataacttacc
     3121 atttatataa accttagaat ccttaatttt tagatggtca ccaggaacac ctataaccct
     3181 ttttacaagc tcttttttcc ttccattctc ttgtaataaa tcagtcttaa atatcattat
     3241 atctccacat tttggttctg ataacttata tgtcatcctg tttgctacca aataatcatg
     3301 tgatttaagt gttgggtaca ttgattctcc atttactaat gttggtttta taaaacgagt
     3361 tataataaat gcaaaaaaaa gtgcagtaat aattattttt atccactcta caatctcttc
     3421 tttaatagtt tcattcattc acaaattctc ctttatagtt atattatctt tattgataat
     3481 gtctcttaaa cactatcttt tagataagtc tactaaataa ggtgttgtct tttttatata
     3541 gacaactatt gtgtatggtc tggtgatttc agctaatcag cgattgctcc aaattttata
     3601 tgtttagtca atagttccta tcttagaaaa tggatacaat ctgactagaa cctttcctaa
     3661 aatggtatgc tcgtcgacca atccaacctc atcgtatcta ctatctaaac ttttttctct
     3721 attatctccc attgcaaata attttccttc tggaactacg atatcaatat ctccatcagt
     3781 acgattatta tgtatatagg aagtctcatc taacaactta tcatttacat ataccttgga
     3841 gtcttgtatt tttacatggt caccaggaac cccgataact ctttttacta aatctttttt
     3901 ctttccattt tcctctgtta agtcggtttt gaatactatt atatctttgt attttggctc
     3961 tcctaccttg tacgcaattc tgttaactat caaataatca cgttcaacta atgtaggata
     4021 cattgattct cctttgacta ttgttggcac tataaaacga gttattgcaa atgccaaaac
     4081 aagagctatg acaatcactt ttatccattc tacaacttct tttttaactg cttcacccac
     4141 taaaatcgtc tcctctgtgt taaatattaa gtcaatctac agcaaaataa tctaccataa
     4201 acctacaata taaatattat acttcttttt tctaatagtt taaaatactt ttctaagacc
     4261 tttaggataa tgattcttaa ttacaccaga agattctttt ccccaaccta aaggtattcc
     4321 ttcaaccgaa accaaaaccc atcctctact atcattagaa tttacaacat ccccttttaa
     4381 atatttaaga attgattcac tctgtacact gaagttttca acatttttta catcttcaac
     4441 ttttaagtaa tgagatagtg catgagaagg ctcaaatcta ttcttcttta aaacccctaa
     4501 atgcaaacca tatctcaata cttttagttt tttcgtatct ggatatattt caggaagtag
     4561 gtacaaattt tctcctctta aataaaattt atttttttta ttactataaa aatcaacctt
     4621 taaaaatttc ttttcaaact ccctataatc ctttatttct ttatctaaat tttttatttt
     4681 taattctttg acattacatt cttcttcttc taattttttt attttggcaa caaaatgacc
     4741 ttctcccttt atcttatgag gccataatct ttccatttca ataagttctg catgtggata
     4801 ttcactgata aactcattta taacatgctc attttcctcc cttgaaaaag tacaagttga
     4861 gtaaattaaa atgccatcct tctttaacat tttatatcca tctcttataa tctctttttg
     4921 gatggattga cattctacta cctttgcata actccaatct gatatggcaa cctcatcctt
     4981 cctaaacata ccttgcccag aacaaggtgc atctataata accttatcaa aatacccatt
     5041 aaacgtcttt cttaagctat tagaatcggt gtttgttata atacaatttc tcgctccaaa
     5101 tcgctctaaa ttttccccta gagccttaat tctactagca ttaatttcat tagaaaccaa
     5161 taaacctgta tcgtttagct tagatagtat atatgtagat tttccaccag gagcagcaca
     5221 catatcgaga actttttcac cctctttaat atctgccttt ggtattacac tcattgcaga
     5281 aggctcttgc aaatagtatg ctccagcctc atgcagtgga tttctaccag gtctgtcaat
     5341 tttatcatca taataaaaac cttcttctgc ccatggtatt tgattcaaat caaacaactc
     5401 caaatcaaat aattttttct tgtcaatttt taaagtattt attctaagcc cagtagtctt
     5461 actttcctca taacttttta taaaatcatc atattcatct tttagtattt cttcaatatc
     5521 ttctaaaaat tcctttggta attctgtcaa atctataatc ccccttcaat ctgctttact
     5581 tgtgtgtcat ttattatact ttaattattt atgtctttcc tttaaatttt atttcttctt
     5641 ctttttccta agattaccga aaaacacttt tttagagtga ttcatatatt tctcaagttt
     5701 atcaagattt atcttccaca aaattattac ataaactata agtaatataa tagcccatct
     5761 atattctatc atcaatatga aatcaaaaac tccatgtaat aaaattggaa ttaacaccgc
     5821 cataaacaaa tactcacgtc ttttattttt atttccctca aatttatact tagaaatata
     5881 gtagcccata gtaattgcaa acattacatg agctggtata gagataactg ccctattaat
     5941 accaacttgc agtgctagat ttctactttc agaaaaaata taaaccatat tttcaattgt
     6001 tgcaaatcct aaagctaaaa atacagagta aataatacca tctaactttt cagtaaagtg
     6061 tttttcttta attagggcag gaatcagtat cagagctttt acaccttctt ctacaagtcc
     6121 tgcaactaca aatgatacat atattagttc tgaataccct tcaaatacat taaacttcat
     6181 cagtaaattt tctaaaagta taattataac acttagaaaa gcacctatta aaaaaaattt
     6241 aattagatgg attaggggtt ctctacaata tctatcttta gaccttatcc aaaacatccc
     6301 tatcaatata ggaattattg ctaataaaaa taaatctagc ttcatagatt attttccttt
     6361 caaaattttt gatttattta tgatttatat catctactta tttttaactt attcgtgttt
     6421 taaatacata ctaaaatata cattaaatta ttttaaatta tttaaataca aatatactca
     6481 aatactcaaa aaaatttaaa tttatatcta tattatttaa caaatttcaa gctatattca
     6541 tatcttgaaa tataagggaa ctaaattcat ttaaagaaag ggtgatttga tgcaagatgg
     6601 tttcttaaca gtaagcataa ttgatgctac taacaataga cctattcaaa atgcagtagt
     6661 aaacatatat tcaatgtcta atggtagcca aagctcatca actttatacc aaaacctaag
     6721 aagtaatgaa tctggtcaag tgactggttt agtattacca gcaccagatg tagattactc
     6781 tctacaacca tctgatgtta gaccttatag ccagtatata gtggaagcca tagcagatgg
     6841 atatgagaca gttgtcatag aaggaacaca gttgttagct acaattgaag caagacaagg
     6901 agttcccatg tctcctagaa caagaagtaa gcgttctttc tcaagacaaa gtgagttgat
     6961 ttttgatata ggagaacata ctctatatgg tacttatcct cctaagattc cagaaagtaa
     7021 cttaaaacca ttaccacctc ctactggttt cgttgtatta gataaccccg tagtgcctga
     7081 atttatagta gttcatgatg gtttgccaga agattcatca gctccaaact attggattcc
     7141 atttaaagag tacattaaaa acatagcgtc atctgaaata tattcaactt ggcctgaaca
     7201 gacaatctat gcaaatgtaa tagctataat atcatttact ttaaatagag tctttactga
     7261 atggtataga aataaaggat ataactttac aataacttca acaacagcat acgaccataa
     7321 gtttataaat aatagaaact tatttgaacc aattaacgta gttgtagatg ctatttttaa
     7381 tacttttata aaaagacctc ctacatcaag acagccacta ttagcacaat attgtgatgg
     7441 acaaaaatcc caatgtcctg accaaatgac tcaatggggc agtaaagact taggtgacca
     7501 aggatatgac tatgagagta tactaagata tttctatgga gatgagatag tatttgaaag
     7561 agcacctata gtaagtggtg taccagtgtc attccctggt actacactac aagttggttc
     7621 ttctgggcag tatgttagaa ccatacaaaa tcagttaaat gctatatcta attcttatcc
     7681 agctgtacca aaagttatag aagatggtat ctatggaaca gatacagaaa acgctgttaa
     7741 gatattccaa ggaatctttg gtttacctca aagcggagta gtagatttta aaacatggta
     7801 tgaaatatcc agagtttatg tagctacaac tagaatagct tctttaaatc ctttaattta
     7861 aagcttgatt tagaacataa actcaaatta ttgaataaaa ttattaattt aaaataaaag
     7921 tggctgttat gaactaatat ataccataag tattacttat aagtattgta gtcttaacag
     7981 ccatttttta gttataactt gtttttttag aatcttaaaa ctttaaatgg gggattttaa
     8041 gcttcttaaa aacagtgctt agcaacttag atttctttaa aatattatat ttatttttaa
     8101 gtttaataaa atgctattct aacgaaaaac ctacttttta tttaaaatct cacttattat
     8161 ttaaaacact atccatcact tagaatatta cttcttatat aaattattat ttattataaa
     8221 tacaaataat attaatttaa aaagttattc ctaatttaaa taatactact atcttcatca
     8281 gtgaacctaa actgcaactt tagacttatt agctaaagcc aatattagac ctaaagctat
     8341 agatgcaaat gcagaagcaa ttactctata ctcagcaggg aataaaaata caattgtatg
     8401 acatggtatc cagaaaaatg gtatagtttt taaaacacta aaaccaaggt aagcattcca
     8461 gtccacaata tttaccacat ctttaagttt taccttaatc ccctttccaa cctcatattt
     8521 tttatctata aaacagtcca taaccttatg gaataaaaat acagttggtg acattagtat
     8581 attcatagtt gcactcttaa atagagcatt tgcaaaagct attccctcaa atggaagcat
     8641 accatccact tgtgcttgac ctacacctga tgagtagatt gacattccat atgtggtaac
     8701 tacacctatt attccccaaa ctattgctct ataaaatact cctactggta ttttccaatc
     8761 cttatacaaa attctaacac caagtaaatc tcccattgta gctaatacag caaactttaa
     8821 aaaacctcca atatatggat gtgcacctgt tgcttccata aatacttctc tactacttgg
     8881 tattgctaag acagcaataa tacaaataag aactccactc catataaaat cgcctttttt
     8941 catatttata aatactattt aatatataaa tagttctcct ttctattaaa gatttattta
     9001 atttacaatc tatctacaat tatttattaa tcacttatta ttagatattc attttccata
     9061 tttaaattta ttctatccct ttatctattt attttcttac tactctatcc accattatct
     9121 atatatttat tttacgatta ctctttcacc ctaacccttt aactatctat tttttcttac
     9181 tattcttatc cacatcatcc atatatttat tttacgatta ctctttcact ctaacccttt
     9241 aactatctat tttttctgat attcttatcc accatcatcc atatatttat tttacgatta
     9301 ctctttcacc ctaacccttt aactatctat ttttttctgg tattcttatc tactattatc
     9361 tgtatattta ttttatgatt actcttttaa cctaccccct atactttata gtaatatgtt
     9421 tttaaccttt ttcattattc ccaatatgct ttataactac aaatactctc cccccttaaa
     9481 aataataata aaattaatca ataaatttat aatcatatta ttactctatg tattaagcaa
     9541 ttactatgcc aataaacttt gattttttta aacaaatctt aaaaatccta aaatgctaat
     9601 tttctttaac ttttaatgaa tctaatagat aacatttaat tttcgttata tttttcagaa
     9661 tatttctatt attattttta agaaaaaaaa ataatgttaa catttcttaa catacattaa
     9721 aaaatgttaa cattataaat tgggtttaat acttttactt tgactcgatt tacttttatt
     9781 ctacttctta ctttattttt aacttagact ttattttttt attacttatt tgagtcttat
     9841 ttattttatt ctcactccat atgttcaatt acatttgtct aaattctttg atacttttat
     9901 tacataattt taacaagctt aagtgttatt tgggtagtgc agttgataat tatttaaata
     9961 atcatcaact gcatgtttat atcattacag tagcttaatg tatttaatca tacttactta
    10021 ggattttgaa atatcttaga gattatattt aacattagtc aattgctgca taattgtaag
    10081 caattgaaat ttgagaccca tcttcatttt ctatagtgat aggcttcata atttttaatc
    10141 cttcagtttt tatcttttct aattcttgtt ccattttctt aattgtttcc tcaaggtctt
    10201 tttgacttac atctttgact gcattagggt ctttagcata gtcttttaat tcttgaatat
    10261 gtttagcata ctcttcataa gtataatgct caacaccata cttttccaat tcagtagggt
    10321 cagttattcc tgattttatt agagattcaa atttaggtaa agcaatatta gatgaatcat
    10381 ttccatttgc aaatactgct ccagttcctc ctagtaccat tgcagatacc atcattgcac
    10441 ttataaggga tttttttatt ttcataacca atcttccttt cttagagatt tatttgggta
    10501 gtgtagttta aaatgactat tgcaatagtt atcaactaca ctcttagact atcacagtag
    10561 tcatgtttat gtcaagcctt tttaaaaaac ttttcaataa aaaatactat ctcaaagcaa
    10621 aattaattat tttgagatag tattttaatt ctaaatcttt tatattacta agatttatgg
    10681 ttattctatc catgttctat attgttcttt attaactgct aacaaattcc cttttcttaa
    10741 tatgacaaag cgattatcat tatagccact tcgctcaatt gaaacttctc ctgaatcagt
    10801 ataaagtaca tccttactat tactagattt atttacatac gtttcttcac tattctcaga
    10861 attagttact ttgctcatag atgattcttc atgctctcta aattcaatta cagatacttt
    10921 tgccgtacta aaatgagtct ctccatcact tttagcttta ctaacaagaa tatcagattc
    10981 tactttggga gaatataaat cagttgattt actacttatt gaaaatccaa ttttatcact
    11041 ttgttcattt tttgttaaac caaaagattc aacctcatta atctctttat tttctgttgc
    11101 aaaaacaaga cctgtcccac aaactaatac agttgaaact aaaaaagtaa cataagttga
    11161 ttttttaaat ttcataatat taactattct cttttctatt tttgtctttc caaatccact
    11221 acatattgtc attgaaaatg gattattctc ctctgctaga cttattagtg tggttgcata
    11281 atcagcccta tattcttcac caagtatact cataaccttt tcatcacaac taaattctat
    11341 gtctttattt agtaaaaaaa acatcaccca aactaaggga ttaaaccaat ggacaacaag
    11401 agctacaagc ataaataact ttaacagatt atcaaagcta ttaatatgaa ccatttcatg
    11461 agttaaaaca aagtctaaag tttgattatt agcataattc atttgctttg gcaaaataat
    11521 tcgtggtttt aaaataccag atgtaagtgg tgtcctagta aattcagata tgcaaacaat
    11581 aaatcttcta aaacggctat tcttacacat ccatttttta atgaactcat gattttttat
    11641 atgaattgaa gttttaaaaa gattaattat tttaaaatat ctaaagcata aaagtatgct
    11701 aaacataata gtaactataa aagacaaaat gcccaacatt tccaacatca taatattttc
    11761 aatattagac attatttcaa attcgtcaga attgttttta cctgtagagt cactagcttt
    11821 cttaagagta taattttctg ttgatgtatt acccaattcc acatcaaaag tcgattcaat
    11881 tatactataa atactaaaac cagaatttaa acttactggt attagaagtt taaataaaac
    11941 aatcaaccac aataatacaa ataccttttt aggtaatttt ttaaataaac atgctcttat
    12001 aaaaataact attaaaagta atactccaga agtaaaactc atttctaaca atttaatgaa
    12061 cataaatctt acctcacttt aatttttcaa caatttgttt aagttttgta atctcatcac
    12121 ttgaaagatt ttctttatta ataaaagctg aaagaaattt ttctgaagac cctccaaaca
    12181 ttttattaat taaagtttct gtttcctgtt tttgtacttc ctctttgctt attaaagctt
    12241 tacaaacaaa attcggctca attctctcta ttgcaccttt tgacactaat ttttttataa
    12301 cggtatacgt agtgtttcta ttccatcctg tttcttcttt taaaattttt actaactctc
    12361 cagccttgct ttctcctctc tcccatagaa cttccattac tttaagttct gaatcaaata
    12421 attttatctc catataaaat cgctcctcaa atatagttat cacaatagtc attactataa
    12481 taaaaaacta tcatagtagt ctatactatg tcaatcattt attttttaat aataaaaact
    12541 gtctttttaa aaaattctta taaaagacag ttttatcaat gttatacttt aatgtttaat
    12601 taaagccata ttttatttaa atatataact atctatctta gtacatagta taagattttt
    12661 ctttatctaa tagtatatcc tctacatctg ggttttcttt tactaattgt ctataaaaat
    12721 tacaattagt aacacccaca tatggaatta gccatagtag acctattcca agagtagcta
    12781 ttgaaagtat ataccatcct ataaagctaa agtttagtac aaataattta agtacatttc
    12841 ctttcatcat atctaaacta gcacccattg atttaaatac acccatatta tccttgtctt
    12901 cacaaattaa gaaagtagct tgagaaacat ataatagcaa tatataaact ggtaacataa
    12961 gaacaagtat tataagaact agagttattc cagtagaaat aatagtccct attccaacat
    13021 tatcacttga taaatattca attgctccac tagtaagtaa aactccaaat gataaaaacg
    13081 ctcctataat tgtgattata aaagaataaa tatacacaat aattgattca aataaataaa
    13141 aaccaaaact tcttaaataa attctcccac ttacaaagat atctcctatc ttgacttttt
    13201 catctttagt aattttcaag aacatacatg taaatcctgc tgaaacgcaa aaagctatta
    13261 cagccactac aatagtagtc aatggttcaa aaggtaatcc tacaattgat attaatacta
    13321 tcaatgcaat atatactgcc atagaaattg cacatatccc ccagtttcca gataactgag
    13381 ctttagatac tcgttttaat tcttgccttg ataccataaa aaacacccct ttcattttat
    13441 aaaaaaactt ttaaaaatat aaataatctt ttaacgtata atattaatat atcatatttt
    13501 cgactatata agatacaatt attcaccatg ttttatcaag atatgccata attgtattac
    13561 tagttttata tagtcaacat taccaaatac atcaaataaa tttgattaat tattcaacaa
    13621 gtaataaaat aatttctatt aattatactt tatttagtaa
//
LOCUS       DAYATN010000106        12967 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.106, whole genome shotgun sequence.
ACCESSION   DAYATN010000106 DAYATN010000000
VERSION     DAYATN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.106"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..992
                     /gene="nth"
                     /locus_tag="ACGI8A_002770"
     CDS             <1..992
                     /gene="nth"
                     /locus_tag="ACGI8A_002770"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFH0180760.1"
                     /translation="DIIEKEEINKKGMNKKDIIEKEEINKKGMNKKDIIEKEEINEKG
                     IDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKGETEKKTKKSSSNKATQKT
                     SKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILSAQCTD
                     VRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELYDG
                     EVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQKTE
                     FALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            1186..1656
                     /gene="trmL"
                     /locus_tag="ACGI8A_002771"
     CDS             1186..1656
                     /gene="trmL"
                     /locus_tag="ACGI8A_002771"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFH0180761.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            1692..2540
                     /locus_tag="ACGI8A_002772"
     CDS             1692..2540
                     /locus_tag="ACGI8A_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFH0180762.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            2828..3832
                     /locus_tag="ACGI8A_002773"
     CDS             2828..3832
                     /locus_tag="ACGI8A_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0180763.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            3958..5070
                     /locus_tag="ACGI8A_002774"
     CDS             3958..5070
                     /locus_tag="ACGI8A_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0180764.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLLTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDFIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            5560..6321
                     /locus_tag="ACGI8A_002775"
     CDS             5560..6321
                     /locus_tag="ACGI8A_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6873 family GME fold protein"
                     /protein_id="HFH0180765.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            6351..7142
                     /locus_tag="ACGI8A_002776"
     CDS             6351..7142
                     /locus_tag="ACGI8A_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HFH0180766.1"
                     /translation="MKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKKILNGTKAEQ
                     RALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSLNIFAKKEIF
                     MRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLIKKDQDEFIR
                     VLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVIRENLNYEDF
                     LISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            7322..7924
                     /locus_tag="ACGI8A_002777"
     CDS             7322..7924
                     /locus_tag="ACGI8A_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HFH0180767.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    8536..8774
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8838..10757
                     /gene="thrS"
                     /locus_tag="ACGI8A_002778"
     CDS             8838..10757
                     /gene="thrS"
                     /locus_tag="ACGI8A_002778"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFH0180768.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            11525..12616
                     /locus_tag="ACGI8A_002779"
     CDS             11525..12616
                     /locus_tag="ACGI8A_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFH0180769.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         5297 a         1231 c         2335 g         4104 t
ORIGIN      
        1 aagatataat tgagaaggaa gagataaata aaaaaggaat gaataagaaa gatataattg
       61 agaaggaaga gataaataaa aaaggaatga ataagaaaga tataattgag aaggaagaga
      121 taaatgaaaa aggaatagat aaaatgattg gtaaaaaaga tgagataatt gatgagaaag
      181 atatagagaa ttttatggat attgaagaag ataaaaaaag tatcaaagta agtaaaaaaa
      241 gtactaaaaa aggtgaaacg gagaaaaaaa caaagaagag tagttctaat aaggctacac
      301 aaaaaacaag taaagaaact aaaagtaagt ctaaaattaa aaatgagtca gcaaatgaaa
      361 aagacgtaaa taagatttta gacgagttag aaaaattgta tccagatgct aaatgtgagt
      421 taaattatgg gacagccttt gaacttttaa tagcaactat tttatcagca caatgtactg
      481 atgtaagagt gaataaagtt acttcagagt tgtttaaaaa gtacaacaca gctagagact
      541 ttgctaattt gagcattgaa gaaattagta aagagataaa gagttgtggt ctttacaaaa
      601 gcaagtctca aaaaataaag gatacatctg aacagctatg tgaattatat gatggtgaag
      661 tacctgatag cttagaaaag cttataaaat tacctggtgt tggaagaaaa acggcagggg
      721 ttgtacttag taacgctttt aatcatccag caatagctgt agatactcat gtatttagag
      781 ttagcaatag aataggaata gtggacgagc caaatcctca aaaaacagaa tttgcactta
      841 tggaagctat accaaaagaa agatggtcac actcacatca tgtattaatt tttcatggaa
      901 gaagaatgtg taaagctaga aatccagaat gtgcaagttg cccaataaaa gaagattgta
      961 attattataa agaacttaat gaaacgaaat aacttttaga aagtaagttg tacaagaggg
     1021 attttctgat ataataatac ttgtgaaaat gttagataaa aaactattat atacatgaaa
     1081 taaaaattat tattaacatt attttatata atagtataat attattgtta ttctgtttat
     1141 aaaataaaat ttgataaata aaagcgatac ttagggagga ttaaaatgtc attaaatata
     1201 gtattagtag aaccagaaat acctcaaaat acaggaaata taataagaac ctgtgcagca
     1261 acaggttcaa ctttacattt agtaaaacca ctgggttttt cattggatga taaacaccta
     1321 aaaagatgtg ggcttgacta ttgggatata gctgatatac aatattacga tagctttgaa
     1381 gaattacaag aaaagtatcc aaatagtaaa tacttttttt ctacaacaaa ggctaaacag
     1441 tcacattcag atgtgaaata tgaagaaaat tgttttatag tatttgggaa agaaacaaag
     1501 ggattaccag aacctttgtt gaaagcaaat atggatactt gtattagagt gcctatgttg
     1561 gatgttgaaa aagctaggtc attaaattta tcaaattcag ttgctattgt agtgtacgag
     1621 gctttaagac aaataggata cccaaatatg aggtagtttc aacaagccta aatgagaaag
     1681 gagcatactt aatgaaaaat ctaaagataa tctgtgacag tttatcggac gtgccaaaag
     1741 atttgttaga taaatatgat atagatatag tttcattaac agttatactg gaggaaagag
     1801 aatacaagga tagaattgat atatctggag atgaatttta taaaaggctt agagaagaaa
     1861 atgtatatcc aaaaacttct caagctactt atgcccaatt taaggaaatt tttgagaaat
     1921 atacaaatga ggatagagag atactttata tagcagggtc tgccacagct acaggtacgt
     1981 atcaaagtgc tgtaatggca aagaatgatg tagatggtaa aatatataca tatgactcta
     2041 acttgctttg tatgggaact ggtttacttg taataaaagc aggagaactt gcaagtgaag
     2101 gaaagtctgt tgctgaaata ttgcctgttt tggatgaatt aaaagagaag gcttttgtta
     2161 tattttcagt agatacattg gaatatttac aaaagggagg tagaatatca tctactaagg
     2221 cagcaatagg aagtatatta aatataaaac cgattcttga aataagagat ggattagttt
     2281 cgcaaatatc tcaagtaaga ggaaagaaga atgtaatttc taagatgatg gaattgataa
     2341 aatcaaattg tggtgatgat ttatcagacc aaattgtgta tataggatat agtgatgatt
     2401 ttaaggaaaa agaacaatta tctaaggtta tgcaagatga attaagacct aaagaaatta
     2461 gatttttcca aattggtgtg tgtataggtg cacatgctgg tcctggtgtt actggtatca
     2521 tatgcttaaa aaataaataa tatatttttg gatgacatta tttatttttt tattaaatta
     2581 ggatagaata ttaattgttg agatgttaaa ttggatttat tttggatgaa attaagtgtt
     2641 gatatattag atttagtttg tagggaagtg tgtaaataga taagaatcta ttgcacactt
     2701 ttttatagta agatttatta aaaaaatttc aaaatgtact tgtaaattgt atataaaaaa
     2761 attataaata ggatgtaaaa gtaaatagga tataaaaata tcgtaaatgt atgaggagtg
     2821 aaatttaatg cgtttcaaaa gaatattgat attgatgtta actgtagcaa tgatagctgg
     2881 aatgttggtt gggtgtgcta ataacccaag taataatcct agctctagca aagataataa
     2941 aagtgatata agcgataaaa gtaattcaaa aaaatctgta tcaatgatac tagatattga
     3001 aggaacaaat aatgaagcta tgaataattc agctttatta gcattaaaca atgctcaaaa
     3061 gaaacttaat atagatacaa acaaagtgga atcagatgat tcctcaactt tttctaatag
     3121 tatagatatt ttatgtaatg acaattacga tttaattata gctgtaggag ctagatttgc
     3181 aaaaccattg gagatggtag ccaagaaata tccaaagcaa caatttgcaa ttatagacta
     3241 tgagtatgac aagcaacctt ctaatataac ttcaatttct tatgaagata ataaatctgg
     3301 atacctagct ggattaattg ctggaaaaat gactgaaagc gataaggttg gttttatagg
     3361 aggaacaaag agttctagta gagataaatt tgaatcaggt tttagagagg gtgttaaatt
     3421 ttctaatagt agtataaagg atatttcagt tgagtatgca gatgttttta aagatagcaa
     3481 atcagtagaa tctatagcta aaaaaatgat ggataatgga gtggatatta ttttttctac
     3541 tactgaagat gattcaaaag ctgtaataga tgcagtaagg gcaaaaaata aaaaagttat
     3601 agcaacgaat aaagaccaac atgagttagc tccagaaaat gttataagtt ctattgtaaa
     3661 agattttgag aatcctactt ataatctgat acaaagtttt gtaaaaggaa attataaagg
     3721 cggaaaggtt atagaaaaca cagttaagtc tggaagtact ggacttgccc aaaatagttc
     3781 tcaaaatatt cctccagatg tgttagaata tgttaataag aacaataaat aatctactat
     3841 aatatccaag aatacttatg agtttagtta tgaggtgaat tggatgttat aatagacata
     3901 tatagaaaga catatgcaga ggtaggtcag acctatctaa atcagggagt gaattttgtg
     3961 aaaataaaaa ggatattgat ggcttgcttt gtagtagttt ttacaatttt tatattagct
     4021 atatgtaatt ttgattttgg gtttgataaa gtaaataaga ataattctaa tatagatagt
     4081 gctaaatcga aacagagtga gtctaaaagt ttgctaactt cagcagaaaa ggataaatca
     4141 aatagtaaaa gtaagaataa agaagatgac acagaagatg attcatatgt aagaaaaact
     4201 attggagtat tgttagatac cgaaaactta aataacgaat catataataa caatgtacta
     4261 tcagccttaa aaagggctga cgaagatttt gatgttagga taaaacttga aaaaccaaag
     4321 acttctaaaa gttttaaaga gagtatagaa aaactgcata agtatgaacc agattttata
     4381 atagcagttg gagccagatt tgcagagcca attaaagaag ctgctactaa atatcccaaa
     4441 cagcaatttg caatagttga ctatacttat aaagttcagc cagctaatgt aatttctgta
     4501 tcatttcaag ataatgttac tggatattta gcaggactta ttgcaggtaa aatgactgat
     4561 actgggaaag tcggttttat aggaggagtt gagggttcta gtagagataa gtatgaatct
     4621 ggatttagaa aggggcttaa aacttcaaat aaagatgctg agttactagt taagtattca
     4681 aatatatttg aaaaccctga atcagctaga actattgcaa aggaaatgag agattctgat
     4741 agagatgttg tattttcagc tacagaagat gatagtaaaa gagctattca ggtagcaaga
     4801 gaaaatggtg gtaaagttat agctattaat aaagaccaat acgatatggc tccagataat
     4861 gtaataagtt caattataaa agatgtagaa caacctgtat atgaactgat aaagaacgtt
     4921 gcaaaagatg gttttaaggg aagtaaagta atggaattta aaataaaaga tggggaactt
     4981 ggcttgactt caaagagcag tagaaatata ccaccagatg ttttggaata cataaaaaaa
     5041 gagtataaca aagtgaaaga tacatattaa ttattgatgt agaagattat aaaaatatat
     5101 tgcttaagtt tagattagca ataaaaagat atatttatta aatttgtgct aactgtacaa
     5161 aatatattga ttaagtttag attagtgata aaaagatata tttattaaat ttgtgttaac
     5221 agtatggaat atactgctta agtttagatt ggtgataaaa ggatatattt attaaattta
     5281 gtctaactgt acaaaatata ttgattgagt ttagattagc aataaaaaga tatatttatt
     5341 aaatttgtgc taactgtacg gaatatactg cttaagttta gattagtgat aaaaagatat
     5401 atttattaaa tttgaaccaa ctgtacaaag ctatactaat taagcttaga ataactatat
     5461 aaaattataa caataagtgt gtgggcagaa aaattctgct acacacttta tttatattca
     5521 cataaaaaat ttatcaaaaa aaagaaaaag gagtaaaaaa tggaatttac aagagaacca
     5581 tttatactag atgacgattt gtgtctagct ttggtggaca aaagaattag ttatctaatg
     5641 gaaaatgaat taaaattaag aaatatagag ataataaaaa caatagaatg taaagaatta
     5701 tatgaaagca taaaatatca cccggatata tgtatttgta atttaggaaa aggagatata
     5761 attgttgcgc cgaatgtata taatcaatac aaagagttat taagtagata taaatttaat
     5821 ataattaagg gagaagctaa tttattaaat agataccctt atgatatacc ttacaatatt
     5881 gcgatagttg gtgactatgc tatacacaat tttaagtata ctgacaaagc aatattagat
     5941 tatattgaaa ataataaact taaaaagata aatatagagc agggctattc taaatgctca
     6001 atctgtatag tagatagaac gtctataata acatcagata aaggtatttg gaaaagtatg
     6061 aagaatacag aaatagagtg tttattgata gaaaaaggtc acataaattt atttgagatg
     6121 aattatggat ttataggtgg atgtacaggg ttgatttcta aggataaact agctttttgt
     6181 ggagatgtta aaaaacatcc tgactatgaa cgtataaaaa gttttgtaga aagtaaaaac
     6241 aaggagatag ttacacttag ctgtgaaaat ttactagata taggttctat agttcctctc
     6301 atgacaagaa aggagcgata actttggttg agaaggataa tatttcttcc atgaaatttt
     6361 tgaggaataa taatgtaaac gatagattaa tatatacgaa tgacattgta gaagtagtga
     6421 tagattctgc aaagcaagaa gtgttaaata aaaaaatact taatggtact aaagctgaac
     6481 aaagagcttt aaatattgat gagatatcaa cggaaataac tagtatgata ataagtataa
     6541 tgaaagagaa acttttgaaa gaatacattc taaaatgcta tgggcgtaca tatcctgaag
     6601 ataaaaataa tatatacata tattcactga acatatttgc taaaaaagag atttttatga
     6661 gagaaacagt atttactagg gtgagaaatt atatagaaga aaatgatttt ataaatgtag
     6721 aaggttttat tagatttaga atgagagaat ttatgaaata tatttctgct ataggtgata
     6781 ttgcagttga ggagtattta ataaagaaag accaagatga atttataaga gttttaaaat
     6841 actttataga tactcaagaa gaaaagattg atttgcttaa agttcatata atggaagata
     6901 atacatttgt tttatacgat aaaaatggaa ataagataga cagtatagat gatgaagaaa
     6961 ttataaatat ggtaataaga gaaaatttaa attatgagga ctttttaata agtaccttat
     7021 taactctatg tcccaaaaaa atagaaatat tggatttgtt aaataataat tgctcaaagg
     7081 aaattataga tactgtagaa gcaatttttg aaaataaagt tagtataatt atggaaaact
     7141 agaatctaag catataaaag ctatccatat aaaattaaaa aaattctttt taaatcgaca
     7201 caaaagactt ataattgtat ataaagctat agtataacta gagttattaa gtataaagaa
     7261 tatctacttg attaatgcaa tatagctggc aaatatacaa ttgataagga gtaattttat
     7321 aatggataat ttaattagga tactgatatt agctgttatt ggtggattta taggttatgt
     7381 tacgaatgta gttgctataa gactgatatt tagaccaata gagcctatta aaatacctat
     7441 tttaaatata gaaatagtag gtcttatacc aaagagaaga gcagaaatag cagcaaatgt
     7501 aggagaaata attcaagaag aatttctttc tatggacgaa atacttgcaa atatcattac
     7561 tgacgaagat aaggaagaag ttgtaagata cataaaagca agggtaaaaa ttataataca
     7621 tgagaaagtt tcatttatcc cgagtggtat aaaaaacatg atacaagatt atctaggaga
     7681 gataattgag tctgaagtta agcaaagtat agatgaattg agtaaaaata taataaataa
     7741 agcaaatgaa cgtatagata tacaaaaaat ggtcgaagat aagattaatg aactagattt
     7801 atatgaattg gaagagataa taataagaat agctaagaaa gaattaaaac atatagagtt
     7861 tttggggctt gtcttagggt ttttaatagg tatagctcaa gggttaatta ctatgtttat
     7921 ttaaatatat gagaagtagc atttatttaa gtactaatta tatttaccta tttgagtgaa
     7981 ttacttatta ataacattta gatattaatt gtatttacct atttgagtaa attacttatt
     8041 aatagcattt agatattaat tgtatttacc tatttgagtg aattacttat taatagtatt
     8101 tagatattaa ttgtatttac ctatttgagt gaattactta ttaatagcat ttagatatta
     8161 attgtattta tttatttgat tgtgttactt attaatagta tttaagtatt tactgtacta
     8221 cttattaacc atattaagta ttgattgtat ttaagtatac aaaacctaaa aagagtacaa
     8281 aaattagaag ttagttttaa taaaaattag ttttagtatt tatctagtca tatctatcat
     8341 gcatgtggat acgttttaaa aattttaaat ttatgaaaat attataatat aactataaat
     8401 caagagatta atttaatata aaaatataag aatattggtt tacagctgat tgagatgaaa
     8461 aataatgggt aattaatata ttacattaat gttgattgat gtataactat ctgttataat
     8521 gaaaatatat taaaaatcat agtgttgaag aggaagagta gagtagaaac cattctcaga
     8581 gaatttgggt ctatggctga gagcccatgt aataggtcta tttgaaaact accttggagc
     8641 tgagcttatt agcagtattt gtatttatac tatacgtgaa agtgctcggg tgacgacctt
     8701 atagtcatca agagggttgt atatacttgt atacgccaac ttgggtggaa ccgcgaatta
     8761 atatcgtccc aaacatattt ggggcgattt tttattacaa aaaatctaaa aattatttca
     8821 ataaggagga aaaaacaatg attaaagttg ctttaaaaga tggctcaata aaagaatttg
     8881 aaaatgctat ttctgttatg gatgtagcta aatccattag tgaaggatta gctagaaatg
     8941 tggtagcagc gtctgtgaac ggagaggttg taggtctgga ccacattata gatactgatt
     9001 gtgacttgaa tttatttaag tttgaagata aagaaggtaa ggaagttttt agacatacat
     9061 cagcgcatat attagctcaa gctataaaaa gactttaccc agaagcaaag cttgctatag
     9121 gaccaagtat agaaaatgga ttctactatg atatagattt agaccataga ttagttccag
     9181 aggacctaga gaaaatagaa gctgaaatga aaaaaatagc taaagaagat ttaaaaatag
     9241 aaagatttga acttcctaga aatgaagctt tagagcttat gaaagaacaa ggagaagact
     9301 ataaggtaga acttatttct gatttacctg aaagtgaaat aatatctttc tacaagcaag
     9361 gtgattttac tgatttatgt agaggaccac atttaccatc tactaaaaag gtaaaagctg
     9421 ttaaattaca aagtgtagca ggtgcatatt ggcgtggtga cgaaaataat aagatgcttc
     9481 aaagaatata tggaacttct tttgagaaaa acaaagactt agaagagtac ttacatttac
     9541 tagaagaagc taaaaagaga gaccacagaa aattaggtaa agaattagga ttatttatga
     9601 tacctgaaga aggtccagga ttccctatgt ttttaccaaa aggtatggaa cttaaaaatg
     9661 aattattaaa attctggaga gaaatacaca gaaaagctgg atatattgag atagaatctc
     9721 caatcatatt aaatagaaaa ttatgggaga cttcaggaca ctggtatcat tacaaagaaa
     9781 atatgtatac agttaaaata gatgatgagg attatgctat aaaacctatg aactgtcctg
     9841 gtggattaat ttactataac tctcaactac attcatatag agatttccca atgagagttg
     9901 cagagcttgg tagagttcat agacatgagt tatctggtgc attacaagga ttaatgagag
     9961 ttagagcatt tactcaagac gattcacata tattcatgtt accagagcaa attaaggacg
    10021 aaataaaagg agtagctaac ttaatagatg gtatatacaa aacttttgga tttgaatata
    10081 atttagaatt atctacaaga ccagagaact ctatgggaag cgacgaagag tgggaagcag
    10141 cagaaaatgg attaagagaa gctttagaag agttaggtct accatatact attaatgagg
    10201 gtgatggagc attctatggt cctaagatag acttccatct aaaagattgt ttaggaagaa
    10261 cttggcaatg tggtactatc caattagata tgcagcttcc aagacaattt gataatactt
    10321 atattggtca agatggtgaa aagcatagac cagttatgat tcatagggtt gcttttggta
    10381 gtatagaaag atttataggt atattaatag agcattatgc tggtaaattc ccagtttggt
    10441 tatctccaac tcaagttaag atacttccta tatctgataa atttatggat tatgcaaatg
    10501 aagttaagaa agagttattt gataagggta taagagttga attagatgat agagctgaaa
    10561 aaattggatt taagataaga gaagctcagc ttgaaaaagt gccatacatg ttaatagtag
    10621 gtgaaaaaga agtagctgat aataatgttt ctgttagatc aagagataaa ggtgaaatag
    10681 gtagtattaa attagatgaa tttatagcta gtatttcaaa agaaattgaa tcaagagaaa
    10741 gtattattca agactaatat atattatatt agtagctaag atattgttat ataagataag
    10801 tttaatcaat atgttgatat gttgatgcat agtgattaaa tttatgaaaa gatgagtata
    10861 attagaggta cagttaatca tatttttatg attaatgtac ctctagtttt ttgtatggta
    10921 gatttattaa taaatatatc atagaagtgt tttgaacatg gaaatattta tgtgcattct
    10981 ttaaataaaa gtttattttt tgagtgataa ttaagtgctt gttggtaagg ttatggctgt
    11041 aaaaatcata actttatttt catatttgtt ccattttcat aaaaattatt atccctttaa
    11101 gaaaatttct taatcctctt gaaatatttc ctttttagtc taaagttaaa tttctttctt
    11161 caaaatgaat atttaaatga actaaaattt aagtacattt tctaatctta tgagtctatt
    11221 tttcatagat aattgcccta taatagaaga atctatgttg gtgctatgtg gtgacaataa
    11281 cagcattgtc agtgattaat taatgcttta taaatgatta attagtgttt tatgttattt
    11341 ttggacttat cctctataca tagattaatt tatttaactt tagatagaga gagaatgtca
    11401 agtacataca cagacattag agtttaagaa agtgtttatg gagcaagaac ttttaaaaga
    11461 ggtagtgact tagtttaatt tcaagaaatg ataaatacaa aagaggaaaa agagaggtgt
    11521 tgaaatgata gaaaaagtag aagaaaaatc taagattatt aaaacatcaa aagaatataa
    11581 gcgaactata attgaagaaa aagataaaga atggaccaaa aaattaaatg atatacttag
    11641 tagaagtagt ttttctaatg atgaattaga taaaatcaag gcattaattc caaaagagat
    11701 tttgactaat gaaaatgttg gagaagttgt gactgaagat ggatatgcta agcctgagta
    11761 tgatgaggta tttaaatcat tgttcacttt aaataatgaa tatgatttac ttgtaaattt
    11821 tataaatgat attctaaaag atgctccata tgctaacagg aatatcaaac cgttcactca
    11881 aataaagaga ataattagag ttgaaactga tccaacaatt aattacattg gagagaaaag
    11941 acctaaactt gatattttgg ctgaagatga agaaaataat catataaata ttgagatgca
    12001 aagagctttt gagaacgatt atttggaaag agctgaatat tatcttagtc gtgtacatgg
    12061 tagaaagtta gaagaaggta aagagtataa agaaattgga aaaactattg gaatacatat
    12121 actaaatcat gtaaaatata atcacataga agattatgta aattgcctga gattaacaat
    12181 ggacgggcat ccagatattt attctagtaa aactgcatta tattttatag agcttccaaa
    12241 aattcataaa tctgactata tagtaaatag aattatgcta tggggaaaac ttattagtaa
    12301 tccttctaac ttagatgtaa gagttatagc taagaatgat tctattatta aaaaagcact
    12361 tgatagatta aaagaattag gtagcaatga agaatattta ttaaatctga aaagaggtgc
    12421 ttatatcatg aataaaagta gaaattttaa agaagagatt gaaagagaga cagcaattag
    12481 agttgcaaaa gaaaaagatt ataaaataat tataatgttg aaaaaaaatg gttttaaatt
    12541 agatgacata ttaaataaat ttaatgattt tgacttaagt gaagatgaga taaaagaagt
    12601 atatgaaaat aattgatgat aaataaaaag tgaacttata taagtttgct gaatcggagt
    12661 ctggaaatat ttatatgtat tttttaatga atgaaaattt attttaaaaa caataattta
    12721 gaagtacagt taattatatt tttatgatac tgtacttcta gttttttcta ttgtagattt
    12781 attatttact aataaatcta caatagaaat gcttttgttg taagaatcat tttatctaat
    12841 gattgttttt tataatagtt aaatttccat ttgtagtgtt taaatataaa tctataacag
    12901 catttctata aatgttataa atcactataa tgattcaact caaagttact gcttaaagaa
    12961 gcagaga
//
LOCUS       DAYATN010000107        12961 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.107, whole genome shotgun sequence.
ACCESSION   DAYATN010000107 DAYATN010000000
VERSION     DAYATN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.107"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(82..1683)
                     /locus_tag="ACGI8A_002780"
     CDS             complement(82..1683)
                     /locus_tag="ACGI8A_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFH0180770.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(1714..2058)
                     /locus_tag="ACGI8A_002781"
     CDS             complement(1714..2058)
                     /locus_tag="ACGI8A_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFH0180771.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            2242..3405
                     /locus_tag="ACGI8A_002782"
     CDS             2242..3405
                     /locus_tag="ACGI8A_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0180772.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSIALYRLIQESLSNSSMHGKATEIRIFITFNTSNLIITISD
                     NGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            3398..4045
                     /locus_tag="ACGI8A_002783"
     CDS             3398..4045
                     /locus_tag="ACGI8A_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0180773.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTIKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            4174..5109
                     /locus_tag="ACGI8A_002784"
     CDS             4174..5109
                     /locus_tag="ACGI8A_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0180774.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            5123..6220
                     /locus_tag="ACGI8A_002785"
     CDS             5123..6220
                     /locus_tag="ACGI8A_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180775.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLIISTILIALAIVKEKYKWREV"
     gene            6223..7287
                     /locus_tag="ACGI8A_002786"
     CDS             6223..7287
                     /locus_tag="ACGI8A_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180776.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            7531..8028
                     /locus_tag="ACGI8A_002787"
     CDS             7531..8028
                     /locus_tag="ACGI8A_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HFH0180777.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(8204..8452)
                     /locus_tag="ACGI8A_002788"
     CDS             complement(8204..8452)
                     /locus_tag="ACGI8A_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180778.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(8518..9534)
                     /locus_tag="ACGI8A_002789"
     CDS             complement(8518..9534)
                     /locus_tag="ACGI8A_002789"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFH0180779.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCVNSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(9592..10359)
                     /locus_tag="ACGI8A_002790"
     CDS             complement(9592..10359)
                     /locus_tag="ACGI8A_002790"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFH0180780.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(10427..12616)
                     /locus_tag="ACGI8A_002791"
     CDS             complement(10427..12616)
                     /locus_tag="ACGI8A_002791"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HFH0180781.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYGISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKKFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEGDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         4400 a         2026 c         1421 g         5114 t
ORIGIN      
        1 aattgttttt gttgttattc caccaattga ttttaaccat aaataagtat ctcttttttc
       61 cataaaatcc acctcaaaaa actatttaaa ataactataa taagcctttc taaatgaaaa
      121 agcttctata atacactgtg aatttatcaa atctctttct tctaaatctg ctattgttct
      181 tgccattttt aaaagttttg tataacttct actgcttagg ttgtatttac taaatatcat
      241 ctcagccgtt tttgaagctt ctttttccag tttacaatat ttaaataaat tgtaagcttt
      301 aatttcacta ttatttttta tattatcttt cttaaatcta tttatctgta tttttctagc
      361 attttctact cttctcttta ttttttgaga gctttcttct tgttttaaag aattaaaatc
      421 tgaatatttt atagaattaa cttctacaaa aatatcaaac ctatctaaca atggacctga
      481 tattttattt atgtatctat caatttcgtt acttctacat ctacactctg tctctgacat
      541 ataataacca catggacatg gattcatagc tgcaactaac agtactctac aaggatattt
      601 tgcactgtac tttactctgg atatatttac atatccatct tctataggtt gtcttaaagt
      661 ctcaagtatt tttctatcaa attcggctat ctcatctaaa aataagatac ctctatgtgc
      721 taaagcaatt tctccaggtc ttgcatccat tcctcctcca attaaagatt gctttgtagt
      781 tgtatgatga ggtgctctaa aaggtctttt atcaattatt cctttagttt catttatcat
      841 acccaatata ctatagacct tacttacttc tatcatttct tctacactta tatctggtaa
      901 aattgtcctt actctttttg caatcatagt ttttcctgag cctggaggtc caatcataaa
      961 catattatga tttccagcag cagcaatttc tgcactcctc tttacaaaat aatttccttt
     1021 tacatcttta aagtcttcat catatttact ttcttcatta ttaatcttag tatatttgca
     1081 atctatataa ctattatctt tttctaattc ttcgttctct tttcttgact tatcatccaa
     1141 aaaagacatt atccttactt tatcgacttt tatctcttcg tttaaaaaat cgacacattc
     1201 ttttaaactt ttaataggaa ttatatcaat ttcatctaca aatagacttt cttttatgtt
     1261 ctcaattggt ataaaaatac gttttatgtt ctgagttttt gcacccataa ttatgggcaa
     1321 tatacctctt acttttctta ttttcccatc taatgataat tctcctataa acatactttc
     1381 tcttatgtaa ttttcatctt tttttattaa ctctcttaaa aggcctatac ttatagataa
     1441 atctaaaaaa gctccttctt tttttatatc tgctggtgat aaatttacaa ctattctcgt
     1501 gcttggaaat ttatatccac tattaagaat tgctgacttt actctttctc ttgcttcttt
     1561 aatttctttg cctggaagac caacaatatt aaaactaggt attccattgc ttacatctac
     1621 ttcaacctta actaaaaagc tgtcgattcc tactaagttg cttgaattaa taatacttaa
     1681 catatttaat cctttccaaa aacattatct tttttaaaaa gcattcatta tgtgatttat
     1741 tcttttttct aataaatata cctctataac atcaaatctt atgggagtgt tatttaagtt
     1801 attttttata atataataat ttgcaactcc aattatcttt tttctttttt tgaagcttac
     1861 agattcacaa gggtagccaa actttatgtt agaccttgat ttcacctcca caaatataat
     1921 ttcatcttcc atttttgcta ttatatctat ttcccctatc tttaatctgt aatttttttc
     1981 tagtatcttt gctccttttg atagcaaata attaactgct actttttctc caaaatcacc
     2041 tttttcttta ttattcatta ttcactccaa tacattaaat tttcactttt aaattatggt
     2101 cagtatacaa ttttactatt caagtttttg gattgatttt atttccatca atattaataa
     2161 atacttctat tgactttatt cttttatgat attattctag ttttcatagg aaaagtttat
     2221 ttttttggag gagtgccttt aatgaacaga gaaaaattag ttgcttttat acgatattta
     2281 tctttattta tattagttgt aagctatttg aaaaatgttg aaaatacatt gtatccggtc
     2341 ataactatga taatattatt tctaattata ataaataatc aagttagatt tttttcttta
     2401 tcaaatgaga ataaatttat acttatttct tactttttag aacttatatt aatttttata
     2461 ctttgtaatt atacaaaaac ttttaattcg atttactttg ttcctttaat attagatata
     2521 tcatttttaa taaaggaaaa atataagtac atattgttct cttttgttat aatatcttct
     2581 cttattatta gtttagataa aaatatatat ttggcactgg agtcaagctc tatattgcta
     2641 attataactg tactttctat atatatagaa aatgagaatt tatcaaaatt atacagtcaa
     2701 aatctttatg ataagcttag aatttctaaa gatgaattaa aaaaagttaa tgctgatttg
     2761 gaaacttatg caagttctat agaagaatta gcaattttaa aagaaagaaa tcgaatatca
     2821 agagaaatac atgatagtgt aggacattct ctttctacaa caataataca attaaatgct
     2881 atagaaaaac tcttaaaaga caaacctcta atttatgact tagtacatga acttagagaa
     2941 tttgtaactg aaagttttca agatgtaaga agggctatat ctgagttaaa acctgttgaa
     3001 tatgaaaatt accaaagttt atttaaaatc aaagaattag ttaaaagctt tattaagctt
     3061 acaaatatta atgttaaatt aactatttca aaaaatactt ggaacttatc cagaaatcaa
     3121 tctatagctt tatatagact tatacaggaa tccctttcca actcatcgat gcatggaaag
     3181 gcaactgaaa tcagaatttt tattacattc aatacttcaa atcttataat tactattagt
     3241 gataatggta taggatgtgg caatataaaa aaaggaaatg gacttaatag tatcaatgag
     3301 agaatatatg aattaaatgg taaagttgaa tttgataatt ctaataatgg tttcacaatt
     3361 agagcttctt ttcctaaatt ttcagggggt gatttttttg aataaaatta aagttcttat
     3421 agtagatgac gaaaaattaa ttagaaaagg tcttaaaatt atattatcat cttataatga
     3481 tttagaaatt gtaggagatg cttctaatgg atatgaagct ttagaatttt gtaaaatgaa
     3541 tgatgtagac atagttctta tggatatacg aatgaaagta tgtgatgggg ttctaggtac
     3601 aaggcttata aaggaatata ataattctat aacattatta attttaacaa cttttaatga
     3661 tgatgaatat ataaaagatg ctatgaagtt tggtgcttca ggataccttt taaaagatag
     3721 ctctgatgaa gtattgcatg aagggattcg ttcttcattt tttggcaata ttgtgcttga
     3781 taaaagtgta gctgaaaaaa tcatgacctc tgaaaaaacg attaaacaag agcatcttta
     3841 tgacatgtac aatttaacag aaaaagaaat atctataatt agactaattg caaatggact
     3901 taataataaa gaaataagtc aagaactatt cttatctgaa ggtacaataa agaataacat
     3961 aactaatatt ttagctaaac tagaacttcg tgacagaact caactagcaa tctttgcttt
     4021 caaaaataaa attgtaatag aataaatcta aaaatataaa gtgacttaag tcactttttt
     4081 tagtgactta tagtaataga attataattc acaattcttt attataatat tatagctttt
     4141 taacttatac aaaattattt tggagggatt actatggata tagtcagagt aaataatata
     4201 acaaaaagat ttaatgataa attagtactt gataacatca gcttttctgt taaaaaaggt
     4261 gagatatttg gacttattgg cccaaatggt gcaggtaaat ctacacttat aaatataatt
     4321 acaaatctta tgttaccaaa tagtggtagt attcaaatca atggtttaga tttatcaaaa
     4381 gattatataa aagcaaagtc tataataggg ttagttccac aagaattagc aattatagaa
     4441 actcttaccc cttttgataa cttagagtac tttggagctt tctacggttt aaagggaaaa
     4501 ctacttaaag aaagaatcat tgaagcatta gaagttacag gtcttactga agtcaaaaag
     4561 aaaaaggtca aaaaattatc tggtggtatg caaaggagat taaatattgg aatagcactc
     4621 ttaaatcatc ctaaaatatt aatattggat gaaccaactg taggtgtaga cccccaatca
     4681 agaaatcata ttttcaattt tataaaagat atatctaaaa aacatgaaac tactgtaata
     4741 tatacttccc attatatgga agaagttgag cacctttgtt ctaaaatttt tattatggat
     4801 gaaggtaaag aaattgcttt tggaaataat gattatttaa aatcactcgt atctacaaat
     4861 acaaaattaa ttatggaaat aaaaaatata aatgcccaac ttatctttga tttaaaaaat
     4921 actaaaggtg ttatatctgt tttagaaaat aattcattat tagaattgga tatagataaa
     4981 aaattacaac ttacagatat actctctgtc attgacaaaa acaattcaaa aataatgaaa
     5041 atttcttatg aagaacctag cctagaagac gtatttttaa atctaacagg aaaaaattta
     5101 agggactaat ggaggtgatt ttatgaaatt atacatgagt gtaaaaacta tgctaaagag
     5161 tttaaaaagc tcctttatac ttaatttaat atatttttta gctttgcctc tgattttatc
     5221 ttggtttctt gggatggtta cagaaagcat gtttcaaaac cctataaaaa cagaatctac
     5281 ccccattgtt atatatgaca aagataacac aagattatca aatgatttga caaagtattt
     5341 gaaaaatgat ttatcttata tattaacagt taaaaaagat gattctaagg cagaattaaa
     5401 gcttactata cctaaaggct atgagagtag tttattaaat gaaaaatcaa atactctaaa
     5461 catagaaaaa cttggaactc gtgatgatat tgcaatattg cttcaagata tattagatac
     5521 ttaccatgaa aaattttatt taaataattc tcagaaaata agttcagaag atttttctaa
     5581 attatttaat aaaaattcta ttgatacttc tataataaat aacaatgtta aacagagctc
     5641 ttatgaatac tttgctttag tttcactagg atttcttgta ataattttta taatgaacaa
     5701 tattttgtca aattatataa gtgaatcaaa gggactttca aaaagacttt attctatgcc
     5761 tataactaga gtacaattct taatttatga ctttgtaggt ttatggattt attcatttat
     5821 attcttatta ttatatgttt tattctttag aatacttgga ataacattta aaggtaattt
     5881 tgccatattg ctcctacttt gtgctttatc atcttatttt atgacttcaa tatctacctt
     5941 tgtaaccagc ttttttagta aaaaatacgg tacagcaata gtttatgcat tactcttttt
     6001 acagacaatt tttggaggta tcttcagtat gataagtgat acatttacta agcttacaag
     6061 tttatctcct acctatttaa taggagaatt gtttagtaat tatgaaacat ttaaaaccat
     6121 agattcaata ggaaatttaa ttttcacatg tttaattatc tctacaatat taattgcact
     6181 tgctattgta aaggaaaaat ataaatggag ggaggtttaa taatgattag attaactatt
     6241 gcaaatataa aaagatatct aaaaaatcat actcttttaa ttaatatggt tatgcttcct
     6301 attatactaa ttttttcact taattttttt atcaacaact cgggaaatca atcaatgtac
     6361 ttttctccag tagcaattgt atctgactct tcaggaaagt atgaacataa gttaataaat
     6421 agttctaaat taaaagaaaa ttcatttaga ttaaatgaac aagataaggc tatgaatcta
     6481 ttaaaaaata ataaggtttc tgctgtattc gttttagata aaaatttttc aaatagtata
     6541 gataaattac aacgacctgt agttaaatgc tttaaaattg aaaatggtgg tggtagtctt
     6601 tgggcagaat cacaaataga atcttttatt actaaatcgt taaaattgaa agtggataaa
     6661 aatatagatg ataaattgac aaagacaaat ataattgata acaaaccaga taagaataaa
     6721 ggttcatttt tagtagtctt cttgatatgc tattttatgt atattaatgc ggcacatctt
     6781 gcttctgatt tatttacatt aaaaaaatca aatgtgctaa aaagacttat ctctacagat
     6841 aataaagata taaaaataat tttcagtata tttttaggac tattttttat tcaatcaatt
     6901 gtatacactt ttgctctatt atgtttttca attatagaag actttaattt gagtctgaat
     6961 gtattgatga ttgtactagc aaatagtttt gtttccactg gatttgtatt ttgggtagct
     7021 agagtattta aaaatgaaag ttctataagt cttatatcta cattttattc tttaataggt
     7081 ctagctatat ctatctctag tctaatacca tcaatggata aattatcctt tatgactaat
     7141 ctttcaaagt tcacaccatt ttattggact atagatgcta ttaaaaacaa tggtagtatt
     7201 ttaataaata taatagctct aatattaatt ggaattatat ttgtaactgc aggaagtctt
     7261 aaactaagag attttgctaa aaactaataa ttattaaata taattagtat ctatttttat
     7321 ataattgtaa aagaatattt caacatatta aattatttta tcttatattt ttctctttag
     7381 tttcacacat caccaagtct aaactctata ttatatatta atatttttga cttgtcaagt
     7441 tatttatgtt ataatattaa tcattagata attaaagcat tgtacctaag ttgcaatatc
     7501 taaaattcaa tttggaggta taactagcat atgtataaaa ttggtgaaag tgtaatgtat
     7561 ccaaaagagg gagcctgctc tgtaaatgat attgtaacaa agaaaataaa tcatgagatg
     7621 caaaaatact atgaattaag tgtcattttt aatagtaacc ttaaaatttc gattccagtt
     7681 ctaaatgcag atagaattgg catacgtcca gttatggatg gaaatgatgt tgataatttt
     7741 atccaatcta ttaataaaac agatggtgta tggatttttg atagaaaaga aagacttaaa
     7801 ctgtaccaag ataaatttca ttcaggagat gtttttgaaa ttgtaaaatt aatcaaaatg
     7861 cttatgatac aagacagttc aaaacaattg tgtagcacag ataaggagtt tttaaataaa
     7921 gctcaaaaat ttgctttatc agaattagct gctgctcaat gtaaatccta tacaatggta
     7981 ttagaggaaa tgaaaaaaca tatattaaat tcaaagaata caaattaaac cagtttttta
     8041 aactggtttt attttttata ctaatcatcc atgctacttt agcttatttt aaactgccta
     8101 atctaatatt aataaagtta tctcaattag atagatgtat tttattcaaa taaaaaaaac
     8161 taagagtaaa tcatattact gatttactct tagctaataa aatttaatct tcttcaagtt
     8221 cttttttctc ttgtaataat acacttaatt catttgcttc taaaacttgt gcctctgcaa
     8281 tatcaagtaa ctcttgttgc cattccacac atttatcttt gtaatcaaaa ggtagcgttc
     8341 tgtcaactat gtacttatca atacagtcct ccgcactttt aattatttct tttctttcat
     8401 caataaattt ttctaattct actaaggctt cttcataagc ttttctactc atataaattc
     8461 cccctttgta caattattct acattaaaat gatttttcct cttttttgta taaaatttta
     8521 tttgtatata acttttccat caatatcttt tatagtttga catccagtta atatcataga
     8581 actacttagc tctgattgaa gtttatttac ataagtttct acaccatcag ttgcaccacc
     8641 aaaagaagca gtcacaaaag gtcttcctat taatactgca tctgcaccta aacctatcat
     8701 ttttagtacg tctaaaccag ttctcactcc accatctgca agtattgtaa ctttaccttt
     8761 aacagcatca gcaatttttg gtaaaacttc acatgctcct ggtgtacagt caagcactct
     8821 tcctccatga tttgatacga ctattgcatc cacacctgct tctactgcca ttaaagcatc
     8881 ttcaacagtc attatacctt tcaatataaa tggtagttta gtagacttta ctaactcctt
     8941 tatttgttta acatttttag gaagtactgg ttttccatgt aatgataaag ttactagacc
     9001 acaggcatct atatcaactc caactgcaaa tgcaccagct tcttcagcta atcttatctt
     9061 ttttataata ttttcattat cccaaggttt aataaataca attccagctc cattatactt
     9121 tttgactaca tctaaatttt ccattaaaaa agcatctact gccgtatcac caaccattgc
     9181 ataaatacca ctattaacac atcctgcaac tactggttct atgtaatctc tttcattaat
     9241 ttttccaccc atatttaaag tagttcctgt tactggagct gcaaatatag gtgcactcat
     9301 tttctttcca aacatctcta tagatgtatc aggatttgat acatcatgta ttactctcat
     9361 atttactttt actttttcta aactcttgct attttctata aaagaagaac cacttccttt
     9421 acctcccatg ccaggcactt cacctgcaca agcaacccca ttacaaacct tacaaacttt
     9481 acaacttcca tttaaatttt ctctagcact ttttaataat tcattatatt ccatctttaa
     9541 tcccccatta actatagtaa tttatattat atacattata tcatattata tttacagtat
     9601 atttttcaaa aaactttttc tgtggatagg agttatgcca tatttttcaa tagcttcata
     9661 gtgttcttta gttccatatc ctttgtgaga tttaaaccca tattctgggt atactctatc
     9721 atattggtac attatactat ccctagtaac tttagcaagt atacttgctg ctgctattga
     9781 tatagatttt gaatctcctt tcacaattgg attttgagat atgtcaatac ctggtattgt
     9841 tgctgcatca actaataaat aatccggtgc tttttttaaa caatttatag cctttttcat
     9901 tgccatatat gtagcattta atatattaaa ttcatctatt tcttcattgt ttactatacc
     9961 tattccataa tctagtgctt cttcttttat tacttcaaaa agttcatctc tttttgcctc
    10021 actcagtttc ttagagtcat ttacaccttc tattttggta tcttttttaa atactacaac
    10081 agaagctaca acaggtccag caagtggtcc tcttcccgcc tcatcaattc ctccaatata
    10141 taaataacct ttatcatagc cttcgttctc aaatatattt atagtttcca atctttcttc
    10201 ttcttttcta atattatcca gcttttttgc cagttttata gctagacctt gtacagattt
    10261 tctttcatca actcttaaca gctctatata ttccatatat ttttctactt ctaaagtctc
    10321 tattatttct ttaatctccc tcacactttt atcctgcata ccattccctc atctttcgtt
    10381 tttaaaaatt taatttttaa atatattaaa attttttaat tagagtttaa ttgcatccac
    10441 tatagccaca ttttaaacat atactacatc cacctgtatg tgcaattcct tcacttccac
    10501 actcagggca cacaaatttt tcatctcctt caaaattttt atttattatt tctttggctt
    10561 tttcatcttt attatctttt tcttttttat tatttttata atattctttt ttactatttt
    10621 cttttttatc ttctttatta ttttcttcct ttttttcatt tattaaaatt ccactccttt
    10681 tacatccatc tctaaatatt gttactcctt taagaccagc ctcccatgcc ttaatatata
    10741 tattgaaaac atcttcaact gtagtttcaa aaggtatatt tattgtagat gatatagaag
    10801 catcaatata cttttgccat actttttgca tgtttattct ttcttcatat cctatattca
    10861 tagcagtaac aaaaaaatct ggtaaacatt cttcatctgt aatacctttc ttgtccatat
    10921 actctttaac aattggagta tatactttat aatacacatc ctcatcatgc aagctttcag
    10981 tttttcttat atatgaaaga ttaaatatag gttctattcc accactgata cctatcattg
    11041 tagatatgga acctgtagga ggtatggtta aaagttgtga atttctaagc ccatactttt
    11101 taaccatttc cttagtgtct tcatttagat tttctataaa aaacttagat ttagaaatat
    11161 tttcatattt atactcacta aatgttccat attctttagc aattaatgct gattgcttca
    11221 cagcttcatt taacatagtc ttaccaatta aatcacatag ttctaaagat tcatcagaac
    11281 catatcttat tcccattttt ataagcatat cagctatacc cattacacct aaacctatct
    11341 gtctatactt atgtacacta tctctttgtt cttgtaatgg atgcaagttc aatccctcat
    11401 ctaagacatc atttaaagct actacagctt ctctaacgca agattttaat ttattaatat
    11461 caaaagtagc atgttcacca aatggttgta ttataaactc tgacaagttt attgagccaa
    11521 gtagacaact tcctccactt ggaagtggtt cttctgcaca aggatttact cctgaaaagc
    11581 aaaagttctt atcttcactc ataaaattat atgtttttat tttatcccaa aacaatatac
    11641 ctggttctgc ataatcccag ttatttttca caaatttatt aaataaatca taagcattta
    11701 ctatcttaac tattttttct ccagtagttt caacttcaaa agtacaatta tataactttt
    11761 tttctcttac agcttgcata aattcatctg ttactcttac agaaatattt gctttagtaa
    11821 ccttctccaa atctgttttt atatctataa agtcttctat ctctggatga tttacatcca
    11881 tagaaatcat aagagctcct cttctccctc tttgaccaat taagccagtt gtcatactgt
    11941 aaagttccat aaaagagact gctccagtcg tatgttttgc agcattgtta actttcgctc
    12001 cttttggtct tagctttgaa atatcaattc caactcctcc accataacta aaagttctag
    12061 caagtttttt tgcagtatca aatatatctt ctatgctatc ttttggtggt tctattacat
    12121 aacaattaga atatgttact ttttttccct ctttctgtaa tcctctattt gctaaaattc
    12181 ttccagcaaa tagaaatttt ttttgtctta ttagttttga tattcttgaa ctattatttg
    12241 aaattctctt tatccattca tcaaaactct caccattatg tctatacttg ttcttccata
    12301 ttgatttttg taattcatca aactcccaag ctttagtcct tatttcagag cgtctttctc
    12361 tatataatat gtagtgtttt gctatatctt ttctatttga ttccataagt agtacttcaa
    12421 ctatatcttg tatttcttct acggataagc atttttccat agaattatag tcattagcta
    12481 tttttcttga tataccatat gccaactctc tatctactcc accttcacta tttatactag
    12541 ctttatagac agcatcttca atcttacttt tactaaaaag ttcttttgta ccatctcttt
    12601 ttattatgta tttcattttt aaacctccaa taaattagta ttaaattaat acacacatta
    12661 actataactc taaacaaact ttttatatat ttgtatattt tcatattaat tatctaacat
    12721 aatatatatt atactatttt atattcaata taacactcct tatagttttc ttaacttgta
    12781 aaatatcttc ctaaaaaact caaaaataat ataaaaaagt atataaaagt caaattatac
    12841 tcttatatac tttatctttt tatatttttt tcattttaac tttgattaat taataagtat
    12901 ttagaaaata aattctttat tttttcttta caaaaatatg tattgatgtt tttatattaa
    12961 t
//
LOCUS       DAYATN010000108        12901 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.108, whole genome shotgun sequence.
ACCESSION   DAYATN010000108 DAYATN010000000
VERSION     DAYATN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.108"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            113..301
                     /gene="rpmB"
                     /locus_tag="ACGI8A_002792"
     CDS             113..301
                     /gene="rpmB"
                     /locus_tag="ACGI8A_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFH0180782.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(461..613)
                     /locus_tag="ACGI8A_002793"
     CDS             complement(461..613)
                     /locus_tag="ACGI8A_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HFH0180783.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(711..1187)
                     /locus_tag="ACGI8A_002794"
     CDS             complement(711..1187)
                     /locus_tag="ACGI8A_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFH0180784.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1406..2167
                     /locus_tag="ACGI8A_002795"
     CDS             1406..2167
                     /locus_tag="ACGI8A_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0180785.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2427..4355)
                     /locus_tag="ACGI8A_002796"
     CDS             complement(2427..4355)
                     /locus_tag="ACGI8A_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0180786.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4589..5041
                     /locus_tag="ACGI8A_002797"
     CDS             4589..5041
                     /locus_tag="ACGI8A_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0180787.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            5044..5352
                     /locus_tag="ACGI8A_002798"
     CDS             5044..5352
                     /locus_tag="ACGI8A_002798"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFH0180788.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5466..6572
                     /locus_tag="ACGI8A_002799"
     CDS             5466..6572
                     /locus_tag="ACGI8A_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFH0180789.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6647..9325
                     /gene="mngB"
                     /locus_tag="ACGI8A_002800"
     CDS             6647..9325
                     /gene="mngB"
                     /locus_tag="ACGI8A_002800"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFH0180790.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9642..11978)
                     /locus_tag="ACGI8A_002801"
     CDS             complement(9642..11978)
                     /locus_tag="ACGI8A_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0180791.1"
                     /translation="MLDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGG
                     AENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDT
                     YDGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVL
                     DTTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSY
                     GVNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYG
                     YGNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTL
                     SRIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNL
                     TYWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINL
                     KEKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQL
                     LDVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNV
                     SHDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSG
                     DVYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENL
                     IINISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTE
                     GSGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11950..12666)
                     /locus_tag="ACGI8A_002802"
     CDS             complement(11950..12666)
                     /locus_tag="ACGI8A_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0180792.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4806 a         1797 c         1673 g         4625 t
ORIGIN      
        1 ttcaatatgt gtaaggcaaa tatatatttc tattgcaaat ttttgttatt tatgataata
       61 taaatatgtt tttagtcttt aaatagttat cattttaagg aggtgtgcgt agatggcaaa
      121 agtatgtagc gtatgtggta aaggtaaggt ttctggaaac caagtatcac actcaaataa
      181 acataataaa agaacatggt cagctaactt aagaagcgta agagcgatta tagatggagc
      241 tcctaagaga gtaaaagttt gtactagatg tttacgttct ggtaaaattg aaagagctta
      301 gtaaccacgt ttaaaagcct acttccgtaa gtaggctttt taatttaatc atttttactc
      361 tttctcataa attttaggat tatacttgaa atccattttg gtaattttat tgttactact
      421 ttcatcaata ctcaccccac actttatagt taaaactatt ttaacaaaaa aataataatg
      481 ctccacatcc aataagtatt aatgcggtaa gtgctgacca aatccagcca ggaagcaaca
      541 ttgacagaat tacagccatt cctgttgcta gacaaagtat tcctagcaat accttagact
      601 tgttatttct catggaacac ctccacatag aacctgtatc tataaatatt ttcatattca
      661 agtttatgcg acagttttcc attttagacc aataaacaca ttatgtaatt ttaatttttc
      721 atactagaat tattactgaa ctccgatata gattctacaa tatccaaaat tccttttttt
      781 ctacttaacc tttcaggtcc actatataaa acaactggta taactttagg aagtttaaaa
      841 tttttatctt ctatttcttc aaggcttttt ttcataataa aacttttcca aatttgttct
      901 ctatacgatt tcaatcttgg gacaatttca gtatccattt ctgtttgaaa ttctaacact
      961 atataatgtg atactttgcc gcttttagaa aataccttat aaattatatc aacttctttt
     1021 cctttatatt cacatataaa agatgtttct aactctatat ttttaagagt taattctttt
     1081 gctattgaga cacttgtaaa attttgaatc aaacttaaaa aactttcttt atctgaatac
     1141 attcttctgt actcttctcg tttttcattg cttcttttta aattcataca gttcctccta
     1201 gactatagac cttctatttt ttagtaaaga tagtctataa attttatttt ttaattttca
     1261 ctagtgagta ttgacagttg tgtgtaaaat atccagtaaa gatatttttt tatattttta
     1321 ttaaaagata taaattttta tattttaatg tattgacatt tacgttgcgt aaaagtgtat
     1381 atttaattta taggaggtac tacttatgga atatacagtt caaaaattaa gtaaactagc
     1441 tggtataagt actagaacac ttagatatta tgatgaaata ggtcttctta agccacttaa
     1501 aataaattct tcaggttatc gtatatatgg tcaaaatgaa gttaataaac ttcaacaaat
     1561 acttttttat agagaattag gaattaactt agaaaatata aaaaacatta taaattcacc
     1621 aaacttcgat agtttaagtg cgttaaaaga acaccataat aaattactag ccaaaagaaa
     1681 gcaaattgat ttactaatag aaaatgtaac taaaactata gctctgaagg agggaaaata
     1741 cactatgaca gatatagaaa aatttgaagg ttttaaagaa aagatgattg atgaaaatga
     1801 aaaaaattat ggaactgaaa ttagagagaa atatggtaaa gatattatag atacatcaaa
     1861 taaaaaactt aaaaatatgt ctaagcaaga ttatgaaaat tggcaaaacc taaatatcga
     1921 aataattagt aaacttaaaa aagcttttaa aactggtgat gcctctagtg aactggctca
     1981 ggaagttgca aaattacatc ataaatggtt gtgttacact tggaacacat actctaaaga
     2041 agctcattcc tctctagcac agatgtatgt atatgatgaa cgttttacat catattatga
     2101 tagagaacaa cctggattag ctaagttttt aagggatgct atagtaatat atacaagaga
     2161 taaataatat aaaataataa aggtggtgtc tcaaaatgaa ctttttagtt catgagacac
     2221 tcttttttgt atgaaatcag atatattttt atcattttat caatgaaaaa caggtttctc
     2281 tctctatttt gagacagaat ctttattgta taataattat aatcaataca aaataataac
     2341 aaattataca aaacttactt atactattta tgctaaaaac aaaaaatatt ttattaaagt
     2401 ttttaaaact tatgtaatat aaaaatctat actatagact tgattatgga aagaaaatta
     2461 tcaaaatcat tacaattaga tatactatct ctaatatcat tgtcatgaat tatctttaaa
     2521 aggatatcat aaatggagtt tatttttata taatcatctt tatttatagc caacatcatg
     2581 attaatttaa cagtagaatt ttcactccaa tatattggtt cattcaatat ggaaatacaa
     2641 atcttagttc ttatagagct taattccata ggatgaggta tagctaaaaa atcatctata
     2701 ttggtagaag ataaactttc tctgtaaaat acagattcag caaatgatgg aaatacaatt
     2761 tcactttgag ttaatttgtt gcacatcaac tttataagct catctttatc tttgatttta
     2821 ggattaacaa taaataaatt tttatcaaag aaattgtcca aaagattact ataaatatgg
     2881 gaattattag tgattgactt agatatattc tctatatctt tttttaatag ttttaaatcc
     2941 actaatacaa taggaatact atcatgattt aaaggaattg tcgatattat tatatctaca
     3001 tctgacaatt cactagctaa aaattgatta aatgatagac attgaagtat atttatttta
     3061 tcatgaaaaa ctttgtttaa ttgaacctct aataacctac ttgaaccata ccctgaacca
     3121 catactaata ctacgttttt acacttaata ttactttgaa aaaatctctc tattccagca
     3181 cctatatgca gggatacata tccaatttca tcttctgtta aagagtaaat actattttta
     3241 aaaacttttt ctatagcatt taaagttatt tcaaatgcta aaggataatt actctttatt
     3301 gtatttataa ggggattcac cttatttaaa ttataagatt tagaattgag aattgattta
     3361 aagtgcagta ctaaatcatc aaataataca ctatcatttc taaggtcaaa agtatattga
     3421 ttgttgatta cttctaaaat ttcttcaaca tactctataa ttttagtatc taccctattg
     3481 gaaacgttat ccaaaagaga acttttagtt ataaaatgat tatatagata aactctgtct
     3541 gcctttgaaa taattatagt atatttactc tcaatataat taaatatatt ttcaacagtt
     3601 gtattcgatt gaaaatctgt taaaacaaca tctaaaacat tatctaagca gtaaccatca
     3661 agtattctac ttacagtaat agctaaatgg ataataaagt tttttagatt aaaatctagg
     3721 aaattgatat taaatttatt aatttcactt aacactaccc tttgaagctc tattaaatct
     3781 acattattaa ataattctat ttctctatct gtaattccaa ttatatagtt ttcaaaattc
     3841 ctatttatca tattattaga aatacatctt cgaatatcta tttccttacc ttcaacttta
     3901 actccataat atggcttaga aactaatatt aaattatact ttgacaaaat aggctttatt
     3961 ctttttatgt catttagtat tgtaaattta ctcacataca agctattagc taaatcttct
     4021 agttttatat atgtattgtt atagagaata tattttaaaa tatattgatt tctttctata
     4081 ggagaatctg gtatttcact tgaatccata atatcatcag atattaatgc tagatactgt
     4141 tggtataatg ctaaattaag aatatctata taatatcctt ctcctttttt tattttaata
     4201 atagcaccat ttttctctaa agttccattt atagaagtaa tatcatttct tatagttctc
     4261 tcactcactt ttaaatgatt acttaaagtt ttacaagtaa ttttatcatc actttcattt
     4321 aataatttaa acaacgtgag caatctcttg tgcataaata tcccccctta tagaatttct
     4381 tctaaattat actatttaca ctcatataat acaattactc taataaaatt gtattataat
     4441 aatttatttc cacttctata tggaaattag ttattatcat aatataaatt tccatgtcga
     4501 agtggaaatt agttatattt aatttaatta ggataatgat tacaatataa atataagtta
     4561 agacattatg aatggagtgg aaaaatatat ggtactacaa gaaaaatata tatttataga
     4621 taaagactta aaaagcaaag aagaagtatt aaattttatt gcagatagtg cttatatact
     4681 aggtattaca actaataaag aggctctttt aaaagacctt ctaaaaagag aaaatgaatt
     4741 tccaacagga ataaaagatg gatttgcgat acctcataca aagagtgtaa atgtaaagga
     4801 agcttccata ctctatatta aaacaactaa cgagttagaa tggaaaactt tagatgatag
     4861 taaggttaga tatgtattta gtttgttagc cccacataaa aatgaaggaa atatacattt
     4921 gaaaactcta tctaaattag ccacatgttt aatggaggat gattttacaa gtgaagttaa
     4981 aaatgctagt gatgcaagtc atttagtaaa atatataact aataaaatga aggaggaata
     5041 attatgaaaa tagtagctat aacatcatgt atagcaggat tagcacacac acctatggca
     5101 gcaaaagctt tagaacaagc tgcatcaaaa atgggtcatg aaatcaaagt agaacaacaa
     5161 ggtgctatgg gacgagtaaa tactataaca aaagaagaag cacaagatgc ggactttata
     5221 cttatagcat ctgaccaaac tatatctgga atggaaagat ttgatggtaa aaaagtgata
     5281 aaagtcaaaa taggtatagc gttaaaaaaa ccagaagctg tcttaacaaa gtgtatagaa
     5341 gctatttctt aataaaaaaa tctcactcta atatagaatg agaaaaatca aaaattaaaa
     5401 aattgtctaa tgtaattata acatattatc aaaaaatata acaaacttat ataggaggat
     5461 taaaaatgag taaaaaaaat aaatctcttt taagtgaact taagggacac ttaatgacag
     5521 gtatctcata tatgttgcca cttgtaatag gctcatcttt agttgtagct atcccaaagc
     5581 ttacagcttt agcagtgggt ataacaagtt tagatgctta taaaaatgta tctggttttt
     5641 atcatattct ttacttaatg gaacaagttg gttggactgg cataggtcta ttaaatacgg
     5701 tattagcagg atttatagca ttttcaatag gtgagaaacc agctatgggt gcaggtttta
     5761 ttggtgggct catagcaagt aatactaatg caggattctt aggagcagta atagcaggtt
     5821 tctttgctgg gtatgtttgt aagtttttaa agaaaaaaat taaaatatct gggtcagcat
     5881 caggaatgat gccacttata ataatgccac ttataacagt tggattaact ggttttttaa
     5941 tgagtgttgt attagcagga cctttaggaa acataaatac ttcattaaat gcttgggtag
     6001 ctgaaatgtg tcaaaatgga acaaattcgg tggtattggc tttaatttta ggcgcaatga
     6061 taggattcga tttaggtgga ccagtaaata aagcagcttg gatggcagga aatgcattat
     6121 tgttagaggg aatctatcta ccagcgatat tggtaaactg cgcaatagtt atacctccac
     6181 taggatatgg tttagctaca ttcataagaa aatcaaaatt ttccaatgca ttagctgaaa
     6241 caggaaaagg caatatagtt atgggtataa ttggaataac agaaggtgca attcctttca
     6301 ctttaacaaa tccattaaaa ttagttcctg ttaatatgat aggatgcgct ttgggtgtag
     6361 gattatcagc tttgttaggt gtacatgcta taatgcctcc agttggtgga ttatatggat
     6421 ttatatcaat aggttctgga tgggcttact tagtaggtgc aatatttgga gctttagtaa
     6481 tagcaatatt atcaacaact ttagtagact tcaatgaaga tgaaactacg gaaaatacag
     6541 aatttgatga aatagagtta gatataatgt aaattaaaaa cttaatttta gataattgag
     6601 aattgagtgt actctcaatt ctcaattaaa ttttgggagg atttttatga aaaagacaaa
     6661 agtacatgta gttccacaca gtcattggga tagagaatgg tattttacaa catcaaggtc
     6721 aaaaatatat ttaatgaaag attttcaaga tatattagac atattggaag aaaaagaaga
     6781 ctttaaatac tttactatgg atgcacaagc ttctctctta gatgattatt taaaatggag
     6841 acctcaggat aaatatagaa taagagattt agttcaaaga agaagattag ttattggacc
     6901 ttggtatact caaacagacc aattggttat atcaggagaa tctataatta gaaaccttta
     6961 ctatggaata gatagatgca atgaatttgg tgaacctatg agggttggat atgtaccaga
     7021 ttcttttggg caatcagctc aaatgccaca aatatacaga ggttttggaa tagatagttc
     7081 attattttgg cgtggagttt ctgatgatat ggtagataca acagagttta tatggaaagg
     7141 ttctgatggt tcaaaagtat tagcagttca aataccattt ggttattact atggaggtaa
     7201 tataccagaa gatgattcag atttaaaaga atatttaaaa gatataatcg taaaacttaa
     7261 ggttaaagct tctactaata acgtatattt tccaaatgga tttgaccaag caccaataag
     7321 aaaaaacctt cctgatatat taaaaaaagc taatgaaata gataatgaaa atgaatacga
     7381 aataagtgat atagaaaact atatagattc agtaagaaat gaaagaaatg aatttacaga
     7441 gcttaaaggt gaatttttaa atgctaaaca tatgagaata cacaaatcca tattctcctc
     7501 aaggtcagac cttaaaataa tgaataacaa attagaaaat tatattacaa atgtacttga
     7561 accgttatta acattaagtt attcacttgg aaatgaatac cctcacctag ttataaaaga
     7621 tatatggaaa cttatgtttg aaaatgcagc tcatgacagc ataggttctt gtaatagtga
     7681 cacaacaaat gaggatgtat acacaagata caaacaagcc agagatttga gtataaattt
     7741 attagatttg catatgagac ttataagtac tcaaatcaaa aataataatg aagaaataac
     7801 attaacagta ttcaatggat tcccagaagt cagaaatgaa gttgtagaat ttgatactta
     7861 tattccaggt caagattttg tgataaagaa taaaaatgga aaaattctta attatataat
     7921 aaaagaaaaa gaagatatta caaattatat attaactcaa acaataagat taaacccaag
     7981 taaaaaaata tatataccca aaactgtata tagagcaaaa attgcacttt cagtaaaaga
     8041 tataccacca atgggatata ctcaactagt ttttgaattg gatactaaaa ataaatgtga
     8101 aatagaaaca agtaattgca aggaaataga aaacaaatac tacaaaataa cagccaataa
     8161 aaatggttca ttagaaatat tagataaaga atctaataag acttataaaa accaagctgt
     8221 aattgaagac aatggagatg atggagattc atacaactat tctcctccaa gaaaagatat
     8281 ttatgtttca tctttagatt ctgtatcttc agtagaagtg ctaaaatcaa acattcaaga
     8341 agaaatgata cttaaatatt cattgaccat accagcttca ctggaagaaa gggctatagg
     8401 taaagtttct gtgaaaatgc cagtaacaat gaaaatcacc ttagaaccta aagaacaaat
     8461 aattaagttt aatgttaata taaaaaataa ccaagcacta tctcatagag taagagtttt
     8521 atttaataca gaaatagcat ctaagttttc tatagcagat caacaatttg gaataataca
     8581 aagaccaaca gtattagaaa aagaccttgc attgtggaaa agagacaatt actcatggca
     8641 agaaaaacct ataactatcg aaccaatgca aagttttgtt actttagagg atggacaaag
     8701 aggaatagca ttaataactg gttgtgtaag agaatatcaa atagttggtg ataatttaga
     8761 tacaatagca ttaacactat tcaggtcatt tggatatatg ggaagagaaa atttacttta
     8821 tagaccagga agagcttctg gagaaaaaat aatagcaaca ccagatgcac aattacttaa
     8881 agagttaaac tttgactttg gtctttacat atataacaat aaatttgatg aagcaaatgt
     8941 agctaataca gctaagagat tcttaacacc tatacaaata tatgaatatg ctgatttttt
     9001 aaatggtaga cttatatttg cattcaatga cgaaaaacaa atatatgaaa atgaatttag
     9061 tttgatgaat gtaaataatt caaatttcac agtaagtgca ataaagaaag aagaaaaagg
     9121 agatggaatt gtagttagaa tcttcaatgg tatgaaaaat gaggatgcta agggaagtct
     9181 taacatcaat aaaaatgtca atgatgctta tagtgcaatg ctcgacgaaa tctatgataa
     9241 tactagcaag cttaaagtaa atactaaaac tgtagaggta aattctttat cacattgtaa
     9301 agtacaaaca atagtaatta aataatataa aatagcttat acaagatttc aaaataaatt
     9361 gctagtacca tgttaatagt aaagccagat attatgctat ttataagctc tgtaacacta
     9421 aataacattt tgtaaaataa aaaggtgaac cttgtatatt aggattcacc ttttttaaat
     9481 aaatcaacat tctgttttaa aataacattt ataagctgtt ttgaaataat gcttacaaat
     9541 tattttaaaa caatacctat aaatttttat ttaaagaata cacataaata tttccagact
     9601 cttttatcct atagacttta taattattaa agaagtctaa tctaattttt gttaaaagta
     9661 attgtaactt taaataaatc accatccaca cttatattca tcttaccacc ctgtaattct
     9721 ataaaacttt ttgctatcgc taaaccaaga ccagaacctt ctgtgttacg tgatttatct
     9781 cctcttacaa atctctccat tatatcatct acactaaaat caatctcaga tgcagacata
     9841 tttttaagca taatatttac tttattatct gtttctaaaa tatcaatata cactcttgaa
     9901 tttggcattg catatttgct tatatttatt ataagatttt caaatactct aaatgtacgc
     9961 tgactatcta atggtaatat tattttttca ttagaaaagt tattctttac tattagagat
    10021 gaatctgcaa gtttgtcatc taattctaaa agtgtctgct tcattagaga tacaatatcc
    10081 acatttacta tattcaaata aacatctcca ctatttgcct tgcttacttc aaacaaatct
    10141 tctattagat gttgcaatct ttgtgacttt ctgtctaatg tatcaatata caacttacga
    10201 ttttcatctg ttatactttc atcttttaat aaatctatat aagtgattat agatgttaga
    10261 ggtgttttta agtcatgtga tacatttgat ataagctctg ttttcatctt ttgactctta
    10321 acctcttcgt caacagcttt tttaaatcca gtttgaatat ttcctaattc gtcttttata
    10381 ggattaaaaa atcctaaatc tttttccata tttacatcta agtttccatc tgaaattttc
    10441 tttgtaacat ctaaaagttg attatattca tatattgtct ttttagagta atctgacagt
    10501 attttaaata atactactga atatattaat gcgagtatta ttccaaagaa ccacatacta
    10561 cacattataa ctataatcaa taaatttatt cccaaaattg taactatagt tttatttggc
    10621 ttttctttta gatttattgt tctaatttga ttaataactg tttttcctgc tttttttagc
    10681 catacaaaaa acattcctgt cacagtatgt tttgtaaaat actctcttat tccaacattt
    10741 aaaatatgtt ttaaaagtat tataaataca aatattattg agaatattac aaaccaataa
    10801 gttaaattca ttaaacttac caatatacta gccattttag cacttatgtc attgtttata
    10861 aggtactcta cataattccc actcaaggta ttacttatta taacagtagg atataaatct
    10921 acaagcccct gtataatagc aagagcaata aagagtatct ctaacggtat tcttgataat
    10981 gtatcaaatc ctagcaactc tttagatttt ctatatggga atgctaaagc tacaataaca
    11041 actatagcta atataatttt taaaatcatt ccagaaatct tataataaga attattttct
    11101 acattgctta tatatgaaat tataccatca tcaaaataag tatttccata accatactct
    11161 acactatcac ctttacttat tactttaggt acagcatata ctaatgttat attttttatt
    11221 ggagataact tatatgaaat tgaatttcct cctaaacttg tatccattaa atcttctctt
    11281 gctttagata acaacatttg attaataatt ttttgattta ctccataaga atccacaata
    11341 tttacttttc catctttatc aaaattcatt acaacgtaga atctatagtt ttcttttata
    11401 tctgtaatct gcttttgtct ttcctcatca ttcatttctg tattttttga ctgattttca
    11461 tcagaatttt cttttgaagc atcatttttt gttgtatcaa gtactcctaa ttctttatta
    11521 tcattaaaaa attcaaaatt gctttcttta tcaactaaat agtactttaa attctttcct
    11581 gacgctttaa tgaaatgctc ccaattttca aagttttcat taaaatctgc attaatacta
    11641 tcatctgtat agtaagtttc tgaaccatca taagtatctt tttctttact tacatttttt
    11701 atatagttca tagtttcttc attgataaat agttttgatg gttgtatcat cttaccttct
    11761 tgttcttcct tcatattatg atataaaaca taacttcctt tatatatact atctacaaag
    11821 ctttcctgtt caaatggatt ctctgctcca cctttagcac ttgcttgtat agtagaatat
    11881 tgactaatca tacctaatga tgctgataac ataagtataa aaactataat agtaactata
    11941 tatttatctt taaatttttt caattttgta tccaacaccc cacaccacct ttaaatattt
    12001 aggatttttt gggtcaattt ctatcttttc ccttatattc ctcacatgta ccataactgt
    12061 atctgtattt acagcattct cattccaaac tctttcatat atttcatcag cagaaaaaac
    12121 tcttcctgca tttctcataa gtaaacttag tattttaaat tctataggag ttgtttttat
    12181 atactttcca tcaatactaa cttcttttgt attttcattt aattctaatc cgcctattgc
    12241 aaacacacta tcgtcacttt ctaccttttg ctctttattt ccaaccatgt ttagatactt
    12301 agtgtacctt ctaagttgtg aattaactct agccaatagt tctaatggct taaaaggttt
    12361 tgttacataa tcgtctgctc ctatattaag ccccattatt ttgtcaactt cctctgattt
    12421 tgcagatagc attattactg gaaaattata gttttctcta agcttcattg taagtgttat
    12481 accatccatt ttaggcatca ttatatcaac tatagctaaa tgtatttctt ggttttctat
    12541 aaccttaaga ccttctaatc cattgtatcc cttaaaaaca ttatatcctt gatttaatag
    12601 atatatttct atagcatctg ctatctcctt ttcatcttct accaccaaaa tattatatga
    12661 attcattcta atctccttta tattaattta taaattttct ttacatatat gattatacaa
    12721 tatgatagaa gtttttccat ttatatatat atttatatct tttgttctgg ttatttgtat
    12781 ttaatgtcca tcttagaaac ctccttctag tttaaattta atattttttc attttgtttt
    12841 atatacttaa tattaaaatt aaaaacttaa gatattacat atacttttct aaagattttc
    12901 t
//
LOCUS       DAYATN010000109        12700 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.109, whole genome shotgun sequence.
ACCESSION   DAYATN010000109 DAYATN010000000
VERSION     DAYATN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.109"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(216..1466)
                     /locus_tag="ACGI8A_002803"
     CDS             complement(216..1466)
                     /locus_tag="ACGI8A_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HFH0180793.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1498..2439)
                     /locus_tag="ACGI8A_002804"
     CDS             complement(1498..2439)
                     /locus_tag="ACGI8A_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFH0180794.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2679..3755)
                     /locus_tag="ACGI8A_002805"
     CDS             complement(2679..3755)
                     /locus_tag="ACGI8A_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HFH0180795.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4128..4643)
                     /locus_tag="ACGI8A_002806"
     CDS             complement(4128..4643)
                     /locus_tag="ACGI8A_002806"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HFH0180796.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(5006..6076)
                     /gene="leuB"
                     /locus_tag="ACGI8A_002807"
     CDS             complement(5006..6076)
                     /gene="leuB"
                     /locus_tag="ACGI8A_002807"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFH0180797.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(6083..6574)
                     /gene="leuD"
                     /locus_tag="ACGI8A_002808"
     CDS             complement(6083..6574)
                     /gene="leuD"
                     /locus_tag="ACGI8A_002808"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFH0180798.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6589..7866)
                     /gene="leuC"
                     /locus_tag="ACGI8A_002809"
     CDS             complement(6589..7866)
                     /gene="leuC"
                     /locus_tag="ACGI8A_002809"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFH0180799.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7823..9565)
                     /gene="leuA"
                     /locus_tag="ACGI8A_002810"
     CDS             complement(7823..9565)
                     /gene="leuA"
                     /locus_tag="ACGI8A_002810"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFH0180800.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTTQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9689..9935)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10083..10529)
                     /locus_tag="ACGI8A_002811"
     CDS             complement(10083..10529)
                     /locus_tag="ACGI8A_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180801.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10550..11269)
                     /locus_tag="ACGI8A_002812"
     CDS             complement(10550..11269)
                     /locus_tag="ACGI8A_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HFH0180802.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11387..12661)
                     /locus_tag="ACGI8A_002813"
     CDS             complement(11387..12661)
                     /locus_tag="ACGI8A_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HFH0180803.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNVKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         3838 a         2444 c         1305 g         5113 t
ORIGIN      
        1 taaaacctta taaatataca aatacaatta tatgaatttt catcctcaat tatagcatag
       61 aatatgacta tctatatcct aaaattatcc atttctaaaa aaaaggtaac tattattaag
      121 aatatcaact tatagaattt atattccaca tacgattttc aatcaaaaaa acaagggcta
      181 aaaagtgaat tgtgcacttt ttagcccttt attaattatt catttatttc atgtaagaat
      241 agaccacatc ttactttagg ttcaaaccat gttgacttag ctggcattat cttatttgca
      301 tctgctactc ttattaaatc ttcaatacta gtaggataca ttgcaaatgc tactttcata
      361 tcttcattaa ctcttttttc taattcttca agacctctaa ttccaccgat aaaatcaatt
      421 cttttatcta ctctaggatt atcaattcct aaaatagcat caataacata gtcttgtaat
      481 atagaaatgt ctaatgagtc aacagggtct tctatttcta ataaattttt atttgctttc
      541 attttatacc acttatcacc taggtacatt ccgaaagttc cttttttgtc aggtttatat
      601 ttttcttttc cttctatttc ttctagttca aaattttctt ttattttatt tataaattct
      661 tcctcgctta aaccatttaa gtctttgata accctattat aatccaaaac cattaaattc
      721 tcatctggtg caatcattgc tatgaagtaa ttaaattctt cttttccagt ataatttgga
      781 tttttagctc tcatttcttt ggctacattt tctgctgaag ctgtcctatg atgaccatct
      841 gcaatgtata gataatctaa tttttcaaat ttatctacta ggttatctat tatatcatca
      901 ttatcaatct tccatactgt atgctctatc ttgtcatctg ttatgaaatc atataaaggt
      961 tcattattat ccataaaatc attgattata tcatctatca ccctttgatg tttgtaagtt
     1021 acaagtatag ttcccgtatt agcatcacag tattttatgt gtttagttct atcaatttcc
     1081 ttttctggtc gagtgtactc atgttttttt attgttccat tcaagctttc atcaactgat
     1141 atacatgcta ctataccaac ttgagctcta ccatccatga tttgtttata tatatatata
     1201 cactctttgt catcttttac tagaatttct tgttttcgaa actcatctaa attttctcgc
     1261 gcttttaaat aaactttttc atcatataaa tctatatttt catctaaatc aatttctgat
     1321 ttatctatat gcaaaaatga atatggattc ccttctgcaa ttcttctagc ttctttacta
     1381 ttcataacat cgtaaggaag tgctgctact ttatctacta tatatttatt tggtctaatc
     1441 gccttaaatg gtttaatcgt tgccattact aatttcctcc taattataca aaatatttta
     1501 taatgctacc cctattagat tgttgaagtc aagaaagaaa tcttgaatca catttactat
     1561 ttcgtctcct attctttctt gagcttctac tgtagatgcc ccaatatgag gtgtaggaga
     1621 aactcttgga tgatttacta agtctaaatt taatgtaggt tcttgttcat aaacatctat
     1681 agcagctgct gataatttac cttcatctaa tgcttttagt aaatcttttt catcaactaa
     1741 accgcctctt gcacagttta ttaaataagc ccctttcttc atcttattta tttctttttc
     1801 agttattact gcccccttat tcttatcaaa tggtatgtgt aaagataaga aatctgcatt
     1861 ttctaaaact tcttccatat cacagaattt ataattgtta aagccttgcg cctcacccat
     1921 tatgtcagta tatataacat tcatacctaa taattctgct ctctttgcaa cttcttttgc
     1981 aattcttcca aaacctataa gtccaagtgt ctttccatta atttctgtac ctttatactt
     2041 tttcttctcc cattttccat ctctcatagt tacatttgca gtatttatga atcttgcaag
     2101 aacaaataat tgacctattg taagctctgc aacagatata ctgcttgcat ttggagtatt
     2161 cataactttt atgccattag cttgagcata tttaacatct atattatcca gacctactcc
     2221 acctcttact atcaacttca actttttacc ctctaatgct ttatctatta catctttagt
     2281 aactttagta gctgaacgta ctacaagtac atcaacatct ttaagtttat ctcctaaaac
     2341 atctttttca taaaactgct ctatcacatt aaaatctaac tctctcaatt ttctcgctgc
     2401 ttctttctct attccatcag taactaatat attatacata acaaactcct ccaattttta
     2461 ttcacttaat ttttccaatt tttatttttt aaagtatttt catcaataaa aaactttact
     2521 taatttaact ttctgtttat ataattttca aaatattttt ttattaaagt catatcaaat
     2581 ttttctattg tttgttaaaa atttaatatg taatttttat aaaatattat ttattatata
     2641 ttctaaattt atcttaaaac atacatattt ttacaatatt atttattaaa acctaatata
     2701 tcatcaatat tatctaataa ttcttgtaca tcttcgacag tatagtcacc catatgtgaa
     2761 attctaaaag ttttttcttt taaatctcca tagccatttg ctatttggaa tcctcgttct
     2821 tgtaattttg aatttaaatt tgatacacta ataccttgag tattttctat aacagtaagt
     2881 gtattggata agtgattttc attggtaaaa atttggaagt gttttttagc ccagtttcta
     2941 actaagtttg ccatgtcttc atgtctgttg aatctgttat ctaggccttc atctaatata
     3001 ttatctaatt ggaaatctaa agcgaacatg tgagaaagag atggtgttga agggtattgg
     3061 tagtttttct tgattaagta ttcatacatt gctaacaaat caagatatac tcctctaaaa
     3121 ggtacttgct tagctctatc gattgctttt tgagaaaatg tgcaaataga cattcctggt
     3181 ggtaatccca gtgctttttg tactgatgtt atacaaatat ctattccaat cttatcaacc
     3241 tcgactttta ttccaccggc tgaacttaca gtatctacac agtagattac atcctcatac
     3301 tttttaacaa cttctccaat ttcttctatt ggatttctta ttccagttga agtttcattg
     3361 tgagttacag ttattaaatc gtattctccg gtagaaagca ccttatcaac catttctggc
     3421 gttgttgcct ctcctaattc aactttaaat atatccgctg ggacattatt agccactgcc
     3481 attttgtacc atctatcacc aaaggatcca caggaaaata ctgctgcctt tttggatgta
     3541 caggaacgaa tggagccttc cattaaacca gttccggatg acgtggataa aagtatcgtg
     3601 ttgtttgtat agaatagttt ttgcatcttt tcacttatac tcttttggag catagatgca
     3661 tcttttcctc tatgtcctat cattggtgta gccatctttt gcaagacttc ttctttaaca
     3721 tcaataggac caggaataaa taatttctta ctcattataa ttcccccctt tttagtttgt
     3781 aatatggata gcgtttttac tgtccttatt tttaataact ataatatcaa gctgaattaa
     3841 ctatgtcaac ataaaattta ttttttatat ataatttatt ttatatattt tatgaaaaca
     3901 ttcattttct accttttcat aagtctgaat tttctgaatt attccctcaa aaaaaatata
     3961 ctttttatct atataaatta atttttttag tatgtaaatt ttacgctatt acgataaatc
     4021 tttttagtat aactaattta tttttatctt gtatttttat tgtatttttg aattttattt
     4081 tttctaaata taaaatttat gttttaaaat ataaaatttt tatttaatca aagttcaata
     4141 tattccactt tttctttcga ttcttcaaaa aaccattctt cattagaaaa atcaccttct
     4201 gcaatttgtc caataggcat tttcatatac tttccagttg ctgtcactgc tatatctcca
     4261 tttggtagaa ttatttctcc tgtaccttca aataattttc tgttttcttt agttattctt
     4321 ccaacaactt ttatagtagc atctgttggt actggtttct tatatttcaa ctctaaagat
     4381 actgttactc cccatatttg gtcatcattt atacttacag ctcttccaat agtctcatcc
     4441 aatattgcag cagacatacc tccatgaact ctcccaggat aactttgatg ccaatccttt
     4501 gtattacaaa ttgatacaag ctctccattt tcaagttcat aaaaatctgc tttaagtcct
     4561 agctcatttt gtgttccaca aactaaacaa gatttactac tattttgctt cttcaaaact
     4621 ttatactttg ccaaaaaatc catctcctaa ccgacattag tagtcgctta atattaaata
     4681 attctaaaat atattatata atattaataa aatccctgca aacttctaat aataaaaata
     4741 ggctacttca atatgataag tttaatttaa agtattattt tccaaaaaat aactttatca
     4801 aaatagaaac tcaaagtaga tataaacctc tttttcgtta ttaaaatagt aaaaatatat
     4861 ttgacttaat ataaaaaaga gtgtctcatt aactaataaa gtttattttg agacacccct
     4921 cataaaacaa attatactac aatagatttt tactaattct acaagttata tatttaaatt
     4981 atattattca aattaaacta taattctaaa tttcatttac tattaattcg cccatttccc
     5041 ttgttcctac aacatttccc acaccattat agatatcaat agttctataa cctttattga
     5101 gtacactttg tacagcatct tcaatacact tagcttcttc ttctaagtta aaactatgtc
     5161 ttaacatcat gcttacagat agaatagtcg caattggatt tacaatatct tttccagcta
     5221 tatcaggtgc acttccatgt attggttcat acataccaaa actatcttct ctaagtgatg
     5281 ctgatggtaa catacctata gaacccgtaa tcatagatgc ttcatcagat attatatctc
     5341 caaatatatt attagtcact attacatcaa attggctagg gtcttttaca acttgcatag
     5401 cagcattatc aacatacata tgtgacagtt ctacatcttc aaactcttta gctaagtctt
     5461 ccattatact tctccaaagt cttgaactct caagcacatt tgccttatct acacttataa
     5521 gttttttatt tctatttctg gctatttcaa aagctctctt accaattctt cttatttcat
     5581 tttcgttata tatttctaca tcataagcat tttcgatacc atcaatgatt tttcttccac
     5641 gttctccaaa ataaatgccc cctgttaatt cccttacaac aacaaaatca atgcctttct
     5701 caactatatc cgttctaagt ggtgaagctt cttttatact ttcatacatt gtagctggtc
     5761 ttaaatttgc aaataaaccc aatgactctc ttaattttaa aagaccagac tctggtcttt
     5821 tatctccctt acatttatcc cactttggtc ctccaacagc accaagcaat acagcttcgt
     5881 tttttcgaca agtttcaaca gttttattag gtagaggttc tccagtttca tcaatcgcgc
     5941 atcctccagc taatacatat tcacaatcga atctatgatt aaatttacaa caaattttat
     6001 ttagaacctt tataccttca tctataattt ccggtccaac accatcacct tttatgactg
     6061 ctatattaca attcatactt cactaccccc tatttgcttt tattgagtta attaagcctt
     6121 catttaatat aatattttgc ataaactcag gaaatggttc tgctttatat tcttttcctt
     6181 ttgttatatt ttttatcaca cctgtagaaa aatctacttc tatattatcc ccttcatcta
     6241 tgttattggc agcttcttca cattcaagta taggaagccc tatatttata gagtttctaa
     6301 aaaatattct agcaaaagta gaagcaatca cacaggatac tccactttct tttattacaa
     6361 ttggtgcatg ttctcttgat gaaccacaac caaagttttt agttgcaact ataatatctc
     6421 ctttttttac cttagaaata aatttatcat caatatcctc catacaatgc gttgctagtt
     6481 ctttataatc agctatattt aaatatctag ctggtattat tacatctgta tctatattgt
     6541 caccgaattt aaaaacactt ccattggcaa tcataaattt tacctccctt aaattatttc
     6601 tgatggtttt gatatttttc cagttatagc tgatgctgct gctacagctg ggcttgcaag
     6661 atacacctct gaatcaacat gccccattct tcctacgaaa ttcctatttg ttgttgaaat
     6721 ggatttttct cctgctgcaa gtatacccat atgtccccct agacatggac cacaagttgg
     6781 tgtagaaact gctgctcctg cctctataaa agtggtaata tacccttttt ctatagcttc
     6841 aagatatact ttttgagttc caggaaatat cataactcta actccatctg ctactttttt
     6901 gcctttcatt atctcagcta ctacttcaag gtctgatatt cttccatttg tacaagagcc
     6961 tattacaact tggtctatct ttatatcctt atctatctca tctactgttc tagtattttc
     7021 aggaagatga ggaaaggcta ctgtttcttt tagttcagct aaatctattt cgtatacttc
     7081 atcatagact gcatcttcat ctgcttcata tatatttaca tcttttgtca tagttgaaca
     7141 cttatgtgaa tttatatatt ccatagtctt atcgtctact ggaaaaattc cattttttgc
     7201 tcctgcttct attgccatat tacaaatagt aaatctatca tccatagata agtattctac
     7261 tccatctcca caaaattcca ttgacttgta aagtgctcca tcaacaccta tctcccctat
     7321 tatatgaagt attatatctt taccacttat ccatttattt ggtttattct tcaaaacaaa
     7381 ttttattgct ccaggaacct taaaccaaca ttttcctgta gccatacctg ctcccatatc
     7441 agttgaacct acaccagtag aaaatgctcc taatgctcca tatgtgcaag tatgagagtc
     7501 agcacctata attacatctc cagcagttac tatacctttt tcaggaagaa gaacatgctc
     7561 tattcccata tctcctacat caaaaaaatt tacaactcct tttgattttg cgaaacttct
     7621 tgtaaattta cattgttctg cactctttat atctttatta ggtgtaaagt ggtctaaaac
     7681 catcactact ttagtcttat cataaacatc ttcaattcct aatttttcaa attctctaat
     7741 ggctactggt gatgttatat cattacctaa aactacatct aaatctgctt cgattaattg
     7801 ccctttctta acttcattta atttagcatg tttggctaat attttctgcg tcatagtcat
     7861 ccccattttt tatttcctcc tcaatataac ttttatttat tgcattcata agtgcattaa
     7921 tagctgctgt atttatgttt tccgatatac ttacaccaaa atagacttca ttattgtgct
     7981 ctattcctac ataagctacc cctcttgaat gtgacccttc ctctaatgca tgttcccagt
     8041 aatatctaaa tttataatct ttcatgccac attgctttag tgcattatta aatgaatcta
     8101 caggtccatt tcctatacca acaatccttt gttcatttcc catataactt attcttgcag
     8161 tcatctcaat catgttagta tcattagaat catcattttc atagtttaat tcatccattg
     8221 attttatttt atacttctta actttataag ggctctcaac ttcaatatat tctttcttaa
     8281 acaaattaaa tattgcttca ctactaagtt cttccccaat tctatcagat tccattttga
     8341 ctacatttcc aaaatcagaa tgcatattct taggtatcat aaatccataa tctgtttcca
     8401 ttatataaac agctcctcct tttccagatt ggctatttat ccttataatt tctttgtatt
     8461 ctcttccaat atcatgaggg tctattggta aatatggaac ttcccagtaa tcattatctc
     8521 tgtttttcat tgctatcatg ccttttctaa tagcatcttg atgtgaacct gaaaaagcac
     8581 aatgtacaag attacctgca tatggatgtc tatcatgtac cataagtttt gtacaatctt
     8641 catatatacc tattatctta tctatattgc taaaatctag ctctgggtct attccttgtg
     8701 tataaagatt aagtcccaca tttactatat ccatattccc agttctttca ccatttccaa
     8761 atagtgttcc ttcaagtcta tcggcaccag ccaataatcc aagttcactt gcagccgtac
     8821 aagttcctct atcattgtga gtatgcaaac ttagtataat agaatcccta caacttatat
     8881 ttttacaaaa ccattctatt tggtctgcat atacgtttgg tgttgccatt tcaactgttg
     8941 atggaagatt tattattact tttttttgag tagttggttt ccaaacatct atcactgcct
     9001 cacatatttc aagtgcataa tccatctctg ttcctgtaaa actctctggt gaatattcaa
     9061 aagtaaattc tgtttctgga tattttttta cttcttcatt aaataatttt gctccttcaa
     9121 ctgctatatc tattatttct tgtttactca tattaaaaac cacatctctt tgaagaacag
     9181 atgtagagtt atataaatgt ataattgctt ttttacatcc ccttattgat tcaaatgttt
     9241 tttctataag atgagcccta gattgtgtaa gtacttgtat agttacatca tctggaatca
     9301 tattctcatc tattaatttt cttaaaaatg tatactctgt atttgatgct gatggaaatc
     9361 ctacctctat ttctttaaat cctgtatcaa taagcatttt gaacattctg actttttcat
     9421 ttacactcat aggtgttggc agtgactgat ttccgtctct taaatcgaca ctacaccaaa
     9481 ttggagcctt aaatatttca ttatctggcc atttacgatt tactatttgc attttatcat
     9541 attttttata ctttccacac ttcatgttat ttcccccttt ttctgttttt tgtattcagt
     9601 atacctcata tcgacgcaat cctgcacaat tcataaggat aaatgtcaat tttatcatta
     9661 gaataaaaaa aacctcatct ctaataaaat tagagacgag gttttgctcg cggtaccact
     9721 ctatttagct ttctaaaaag ctcccttagt aatagccaat acaataatta taatgactat
     9781 atttcctata acgtggaatt actccgatta ttctaagatt taaatcttag aaaacagctc
     9841 ctagacaagt tcagaactat caactactaa atcacaccat cctttagctc tctgaaagtt
     9901 tcattgttct tactacttct attcatcact tttttaatca tttaaatttt ctaaatattt
     9961 tgatttaata gttattatat acacattaaa atgtattgtc aacacaaaaa ataattatct
    10021 caatttttta tttttgataa gcaattctat aaaacatgct aactattgaa aacagtatga
    10081 tattatgcct taaacttttt catcttttca tctatgatat tcaataattt tttttcatct
    10141 tccaaactca atgcccttaa tctaatttta taattagatt cgtcagtgta tatatttaat
    10201 atatttttta ctacttctat tttttctatt atcttataat taaacttttg actttgttta
    10261 gattttatac tgaatataca ttgattattt attaatattg aatgattatc gagagtaata
    10321 tatacttttc cctcatattt atttgaaaaa tcacgacctt gtttaaatag aaaaactgct
    10381 gttattatac ataaaatata taatattgtc gttgaatata taaaaattgt agctaaaatt
    10441 ataagtgcta aaaccatata tatagtcatt ccttttttaa aataaccatt gtcataataa
    10501 ttcatataga taaacttatt tttactcaaa ttttttcact tccaagattc taaaaattta
    10561 tatcaaaaaa ttctataatt tcatcttcat gataatttat gactaattca tctggaaaat
    10621 ctactgcttc taaaactttt tctgcatttt caaattctcc tatagaataa catatatgag
    10681 agtcagtacc aagaattact cttgcaccat atgttttaca caatgtcaac atatgactta
    10741 cattttccca agtacccgtt ctatgagagt ttgaacttaa tgatgaatta tttatttcta
    10801 aaaggatatt tttatcttta gcttttttta ctataggttc ataatctaaa ggatacctac
    10861 tgtcatctgg atgtccaatt atttttacct taggtttatc cataactttt aaaatagcat
    10921 ttgtattttc ttcttttgta ccagaagcta tacaaggtct atgcaaactt gcgattatat
    10981 aatccaaatg ttgcaacata tcttcttgaa catctaaatt accattataa tccattatat
    11041 ttccttctat tcctctaaga atctttacgc cttgcacttc acgtggaagc aaatgcaaat
    11101 tataaaaata gaatggatgt ggtcctcctg gcatattagg tccatggtct gatattccta
    11161 agtattttat attcttctta ctcgcctctt cgacattttc cttgaaagta ctatgtgcat
    11221 gtccacttat tagagtgtga caatgtacat ccataataga ctttttcatt aaatttcctc
    11281 ctaatatttt acataaattc gtgaaaatag ctaaagggct atttcaaaac taaagctgtc
    11341 ataatagaca gtgtaacgtt ttaaaacagc cccattttca attattttac acttctaaat
    11401 ctttaaattt atctttaaat aattctccaa tacttcctaa agattcctct acttctaggt
    11461 attctgttat atcctcttct ggctctctag ttgcttcttt tattgaaacc aacattctct
    11521 tagtctttgg tttaaaatca agaatcttaa ctttaacctc atctccaata ttaacaacat
    11581 tagtaacttt agccactcta gtatcactca attcagatat tggtaaatac gcatctacac
    11641 cttctttaat ttgaacaaaa gccccttttt ctattattct aagaacttta acattgacaa
    11701 catcatcaat ttgtacttct tttgctatta aatcccaagg gtcattattt atgtctttta
    11761 atgccaaggc aattttttta gtctctttat ttacatccaa cacataaact tctacttctt
    11821 gtccctctga aacaacatct tcaacacttt caactcttgt ccaagctaaa ttatttatat
    11881 gtgctaatcc ttcaatccca ccaatatcca caaatgcacc ataaggcatt atctttgtca
    11941 ccttaccatc tcttttttgt ccaacttcaa gagaattaaa taagtcttct ttagctgctt
    12001 tgatacgagc tttttcttct tctctttctt gcttaattct tgctctttca gcttcttttt
    12061 ctgccttaat ttgagctctt ctttcttctc tctctttatt tatacgctct tgtaatacat
    12121 ctctatgaga tgctacaagt ctattttttc taacatccaa ttcttttata taaacttcta
    12181 agttaagacc aacaaattta gatgtatctg taataaattt tgtatcaagt tgagaaattg
    12241 gtataaaaaa tgtatatgta ttgtaattag caaatacacc tttatctata ttcttttcaa
    12301 catttacagt tattattcta tgttcatcat aagccttttg taactcggca aaatcatttc
    12361 ttttgtcaag ttgtaattta gataaagtta ttgtaccatc tttttgagat acttttgtta
    12421 ttacagcagt tatttcatca cctatgtggt atatatcttc tatatattga tttttttcta
    12481 tgttcaattc acttataggt attattccat caaacccata gtctaatcct aattgtactt
    12541 catcatttct cactgcagtt atttttccag ttgtcaattc tccaactttc actttgctga
    12601 atacttgttc ttgctggtca agtaattctt gcattgttaa atcattttcc attataaaca
    12661 tcctttcctc ttattgatta tcatatctgt aaatattata
//
LOCUS       DAYATN010000110        12675 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.110, whole genome shotgun sequence.
ACCESSION   DAYATN010000110 DAYATN010000000
VERSION     DAYATN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.110"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(377..1099)
                     /locus_tag="ACGI8A_002814"
     CDS             complement(377..1099)
                     /locus_tag="ACGI8A_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFH0180804.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1158..1835)
                     /locus_tag="ACGI8A_002815"
     CDS             complement(1158..1835)
                     /locus_tag="ACGI8A_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFH0180805.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1863..2678)
                     /locus_tag="ACGI8A_002816"
     CDS             complement(1863..2678)
                     /locus_tag="ACGI8A_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0180806.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2904..3114)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3706..5772)
                     /locus_tag="ACGI8A_002817"
     CDS             complement(3706..5772)
                     /locus_tag="ACGI8A_002817"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFH0180807.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(5849..8878)
                     /locus_tag="ACGI8A_002818"
     CDS             complement(5849..8878)
                     /locus_tag="ACGI8A_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0180808.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(8931..10391)
                     /locus_tag="ACGI8A_002819"
     CDS             complement(8931..10391)
                     /locus_tag="ACGI8A_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFH0180809.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(10483..10857)
                     /locus_tag="ACGI8A_002820"
     CDS             complement(10483..10857)
                     /locus_tag="ACGI8A_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180810.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(11033..11773)
                     /locus_tag="ACGI8A_002821"
     CDS             complement(11033..11773)
                     /locus_tag="ACGI8A_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFH0180811.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(11794..12582)
                     /locus_tag="ACGI8A_002822"
     CDS             complement(11794..12582)
                     /locus_tag="ACGI8A_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HFH0180812.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
BASE COUNT         4021 a         2287 c         1223 g         5144 t
ORIGIN      
        1 ttttactata aaagttaaat ttataagctt acttgttata aaaataactt ttataattta
       61 tttcaaaaaa aatattgatt tttgtgtcgc attttgttat aataaattaa ggttatcccc
      121 ctggtaaccc tagttagtta atatatctat tcccaatacc ctttcataag gatatatttt
      181 atatttatta aaaatcggat tagtaacgga aattaattcg gtggaaaaaa agagcttacc
      241 ccgagctctt ttttttctgc aaaaaatcgt aaaaataaag ttaatattaa tataacttta
      301 attttaaaat taatattaac catcactaaa aaaggatgta ttgaatttct aatatcaata
      361 acatcctttt tatatattat agcacttttg ctaaaaatgc ttttgttctt tcatgttttg
      421 gatttccgaa aacttcttct ggagtattat cttcaagtat acttcctcca tctataaaaa
      481 taactctatc tgcaacttcc ttagcaaatc ccatctcatg agttacaatt gccattgtca
      541 taccttcttt agcaagttct tttataacat ccaaaacttc gtttaccatt tctgggtcaa
      601 gagcagatgt tggctcatca aacaacatca tatctggttc catagcaagt gctctagcta
      661 ttgctattct ttgcttttgt cctccagata attgtgatgg atatgaatct tttttatcta
      721 aaagaccaac tctactcaac aaaatttctg cttttttttc tgcttcttcc ttagtcatct
      781 tttttaattt aattggtgct aatgttatat tatctaaaat agtcttatgt gggaataaat
      841 taaagttctg aaacaccata cctatatttt gtctaacctc atcaatatta gtttttttat
      901 cgaccaaatt ttttccttca aatattattt ctccacctgt aggtatttct agtaaattca
      961 tacatctaag aagtgtactt ttaccagaac cacttggacc tactataacc attatttcgc
     1021 ctttagcgat ttctaagtct atatttttta atacatttaa atctccaaaa gatttactta
     1081 aattttttat tgtaatcata ttatcacttt cctttttact attctatgtt ttccctaaat
     1141 gctaaacatg ctctcatcta gttagacaca gatagtttct tttctaataa tccaactaat
     1201 cttgatagag taaatgttat aacaaagtaa attgctgctg ctactattaa tggctctaat
     1261 gctctaaatg ctgcattttt tactatatca gcagtacgca ttatctcaac aactcctatt
     1321 acagatatta ttgaagattc ttttacaagt gtaataaact catttgctaa tgaaggtaat
     1381 atatttttta tagcttgagg tattattata tatctcatag ttgaccaata atttaatcct
     1441 aatgatctac ttgcttccat ttgtccatta tcaactgatt gtatacctga tctaagtatc
     1501 tctgccacgt atgcaccaga gtttactgat aatgcaaatg cactagctat aaattcactt
     1561 ggtataccaa acaacatagg gaatcttatt cctaattgag gtaaaccaaa ccatactata
     1621 tatatctgta ctaataatgg tgtccctctt aaaacctcaa tatagattga tgatattgct
     1681 cttaatacct tgctctttga tatttttgcc atacagataa ttatacctat tataaatcca
     1741 aagcataatg ccacaagaga aattgatact gtcacacctg ttccttccaa aaaagaagtc
     1801 ccaaatctac ttaaaaaact aaaatctaaa ctcataattt catctcccac ataactttct
     1861 tactaatttg aagcagcttc agtagatgct tcatttaaaa attcttctat cttcttgcca
     1921 tcttgtaatt cttttatctt tttgtttaat aattctataa aatctttgtt attgtcagat
     1981 tttttgattg ctgctgccat accttctgtt acatctttcc caacttcagt atttgccatt
     2041 tttattccat catatttttt aacatttatt aaagaaactg cttcattagt aacaactgca
     2101 tctatgtttc cattttttaa ttctgtaatt aaatcaggta ttgctttcaa agattttgta
     2161 gttgtcattt ttaaagtgtt aactacatat tcttcttgga tacttcctgc ttgaactcca
     2221 actctaagtt tctttaaatc atcttcagtt tttactttgt ctatatctgc atctttaact
     2281 ataactgcat ttctacttaa ataatataat tctgaaaaat caacactctt ttctctctct
     2341 ggtgttggag acatacctgc aagaacaata tcaacttggt ctgcatttaa agcacctatt
     2401 aatccatcaa atgacatatc cttaatttca acctttacac caagctcttt tgcaaattct
     2461 tcagccaaca ttatatcgaa tccttttatt gaatcttttc catctactac cttatgaaat
     2521 tcaaatggag gaaattctgc actagtacca acaactaatt ttccattgtc ttttatttgt
     2581 tctaactttg ttttttctcc tccaccacta catcctacta gcgataatgt caatcctaat
     2641 actaatccta aacttaataa ttttttaaat actttcattt taactacccc tctcttttaa
     2701 ttataatttc cctcagattt taatacttaa atgtttcgag ttacaaaaaa agtctctcag
     2761 tctttaatga ctaagagacg actttaccgc gttaccactc ttattgataa atacttatat
     2821 tactttcaaa atttgtgact gttagcattt aagctgtatg gatactttta tttttaaatc
     2881 aaaaaagtcc cttagcttta cagctaagag acgaattata tccgcgttac cactcttttt
     2941 gataagtaag tacttatcct ctcaatccct taaggcgggt aaaccaatta tcatactaaa
     3001 gtttctgata atctcctcca gagctctctt cacatagact tcattgctag gcttacacca
     3061 acccctaact cgctgtaaat ccatatatgc tactttctct atcattggat ttgtttcctt
     3121 aaattgttaa tacaattgta cttccttata atatttttgt caataagttt tttatttttt
     3181 taataatttt tacttgtaaa ttatctaaat actactgttt atatttattg atattagttt
     3241 tgtaattata aatagtatat ttcatatttt tagatatttc ttaaataata ctaaataata
     3301 tctttcacat tgttgctttt tcttaaacaa tactaaataa tatctttcac attgttgctt
     3361 ttcttaaatt acactaaata atatctttca cactgttgct ttttctttaa taatactaaa
     3421 tagtatcttt cacactgttt ctctttcttt aataatacta aataatatct ttcacattgt
     3481 tgctttttct taaattacac taaataatat ctttcacatt gttgcttttc tttaataata
     3541 ctaaataata ttttccacat tgttgctttt tctaaacaac tataaataat atattaaacc
     3601 cttatttctt tttctaataa gttaaataat acattttaca catatctatt tgtcttgcac
     3661 ataataaata atacattata tttctatcta aatttagatt ctaaattaaa tagtataaat
     3721 tatattcttc aataaacaag ttttcaaatc caagattcgc tctctatctt taaactttat
     3781 tgtaaacata attccatctt ttttaattat ttctccttca tgaaattttt tatggtatac
     3841 cttatcccct acaccaatac tgtcaatttc ttttttcgtt ggagatttta tatcattaat
     3901 aaataatgat ttatcaactt tcttaccata cttatttaaa gtagagctta tacataactt
     3961 ttcttcagct cttgtaatag caacatacat aagtcttctt tcttcttcta attgttcttc
     4021 ttttttctct tccttacaaa tatcatatga tttttcatga ggtattgttc cttcatttac
     4081 acctataata taaacatttc taaattctaa tccttttgca ctatgcatag ttgtaaatat
     4141 gacaccctca gtctgcttat tcttgttatt ttccgttaac tcagtcttta ctctatcaat
     4201 atgctctaaa aactctgtaa cagtattaaa attagttgct gaactctcaa gttcatttaa
     4261 tatctctaca agtccattag tctttatctt tctgttgcta caataatcta aaacatatct
     4321 atcataatca agactagttc taatataaga tattgcattt ttaggattta aagtatttaa
     4381 ataacttaaa tctatttcca aatcatttat tgttttaact tgtttaggat gaagattaca
     4441 cttatttatt aatgctgtta taaagtcttt ttcatcctta accatattta tactgtcttt
     4501 ggatatatat ctaaaaggct tgtttataat tctcaaccaa tctttatttg attttggatt
     4561 aatacctatt cttaaatagg ctagtatgtc ttgacttgcc caatggtcat atattgtaat
     4621 tactgagtct tttactacaa atggaatcct catatccata aatacatcta ctaatgctct
     4681 tgactgtata ttagttctat aaattactgc aaaatctgaa tactctacat aatctttttg
     4741 tatttcgtca attatttccc tggcaatata taaggcttct tcctctgaat catgcggaga
     4801 aatataggta acacttcctc catccccctg actacactta atgatttttt catatctatt
     4861 tttatttttg tcgataagtc tatttgcagt atgaactatc tcacttttag acctgtaatt
     4921 tatatctaat attatttttt tagtattatt aaagtattgt tcaaattcaa gcaaaaaatc
     4981 tggtctagca cctctaaatc catatatact ttggtcttca tctccaactg caaatatatt
     5041 gtttaatggg ctacaaatta actttaaaac ttcaaactgt actttattta tatcttgaaa
     5101 ttcatctatc aatatatatt tatacacatt tctaaccatt tctagtgcag acttattgtt
     5161 caaaagtaaa taataagttc ttataagcat atcatcaaaa tctattttat taactttaga
     5221 tttttgttct tcgtataagt tgtatacttt taaaaactca tcttttgtta atacttctga
     5281 attaaattca tttttatcca taagttcatt ttttacatag gaaatttcat ttataacttg
     5341 tcctatattc tcatcatcat cagcattctc tatatttaaa ctttttagga ttgccttgat
     5401 tgtcattcgc tttgactttt catcaaatat actgtctaag ttgtatcttt caaagtatct
     5461 tagaatttta aaaaatacag agtgaaaagt accataggtt accttattaa gtcttatgtc
     5521 atcacttaaa ttcaaagctc tatttttcat ttctattgaa gatgccttgg taaaacttat
     5581 tgccaagatt cttgttggtg ctatgttttt gtttacaacc atattggcta ttctatatgt
     5641 aattactctg gtcttacctg aaccaggccc tgcaagtatc atgcaaggcc catctatatg
     5701 ctcaactgct tctaattgat tttcatttaa ctttcttata tctatatttg ttgtgattat
     5761 tagctccacc accttgattt ataattatat tattttcttt ttaattataa actaaaagtt
     5821 cataaaaata aatttaattt ttcctctatt aaaccacatc attcctattt tcatgacata
     5881 tcttgagttc ttttttaatt ttgtcgctta tatcttcagg tataaatgat ggtttactaa
     5941 tatagtagcc ttgcatataa tctactccaa gattaattag tgtttccatt tcaccttttg
     6001 tttctacacc ttctgctatc actttaatat ttctttcgtt agaatatgag ataagatttt
     6061 ttaatatttt ctgtctgttt tcatctttat caatacctct tacaatagac atatcaatct
     6121 ttacatagtc tggcattata tataatagta cagcatctcc gttgtaacct gtcccaaagt
     6181 catctaaagc aatttctata ttccaatggt ctatatatgc tttctttttc tcaataaaat
     6241 cttcattaat cttatcattc tctgttattt ctagtactac tctatttaaa tattcaccat
     6301 ataaatcttc aatttcctca aaatctactt ttgacaaatt attattagga atagaattta
     6361 agaacagttt gcaatcgcca aatttttctt tgtatttttt aaatgattta agtgctttaa
     6421 accatgtaat acgctcaatt tgatataatt tagactgtga acgagctaat ctaatgacat
     6481 ctattggcga actaaaagtt tttattttag gtctcataag tgcttcatat gcaaaaactt
     6541 tacctttaca atctacaata ggctgaaatg catactcaac taattgacca tcaattaact
     6601 tgtttaattc ttctctatta ctcaataaga atgaatcctt attatatata ttaatatcaa
     6661 attcactaac ttcacccttg attgtatttt taatttgata catagcaaaa tctgcgtatc
     6721 tcattaattc atcatagttg gttgagtcat cagggtacca ggaaattcct gctgatgccc
     6781 ttattttaaa ttctgttcca tcatgaagtt ttaataaggt attattcatc tgtttcttaa
     6841 cagaattaat aatatctctt atactatctt tactttcata tccatatata aatacataaa
     6901 actcatcacc tgaaagtcta gatactatac cattccatga tataaacttc ttaagtatgt
     6961 ctgcagcaca cctaatatat tcatcaccag catcatgtcc atatgtatca ttaatatatt
     7021 ttagattatc taaatcccac ataataaatg ctgctatctt aagttcatct ttattcttaa
     7081 atttttcttg tatctgtgaa tgaaaagctc tcctatttaa aagattcgtt aaaatatcaa
     7141 aatctctttc atattctatc ttccttttct caataaattc ttttgttaca tcaacaacaa
     7201 caccaagaac ctttagctca tcttcaaaga tattcattct tatccactta agttttttat
     7261 ctttagattc aaacttatat atatatgtat tttctgtttc ataactttct tgcaaatatt
     7321 tatctagtcc ccttaatttt ttcaggaact tatttttttc catatatcca tattcttcaa
     7381 atttgatttc agtagtaaac acgctaaaaa attcttttgt acaaaaaaca ttctctgcgt
     7441 ttttgtataa ctcaaaagct ccaattggca tatctaccat ttcaataatc tgtgataatt
     7501 ttgacgaaga atctgcaaca tttgcactga gcaactctat agctgaagat aattcatcta
     7561 tttcagatat atttatctta tcaagattta ctggttttga tggattactg tttctaactt
     7621 tgttggctaa aagaacaatt ggttttgtaa ataatcttga tgcaatataa atacctatta
     7681 gacctattaa taaagaaatt gctatagata ttataactaa tttctctacc ctttttgaaa
     7741 atgacaaaag acttttttct tcaataatac ccaaaagagc ccaacgttca ttttcaaatg
     7801 gagtattgga gttatataaa tctaagtatt gtacactagc ataaatgttg ccattaccac
     7861 attcattatc tttattttta atcttatata tatttttata cgattccttc tgctctaaat
     7921 tagaatgtaa accatcccaa aatttttctt ttaatgagta tccatttgaa attacatttt
     7981 caaaggccat atcattattt ctatctacac ctaaaatata attacccttt ttattcccat
     8041 ttatctctga gtatggaagt aattctctta aataatttac acttaaatct attccaagta
     8101 ctccatatac agttccatct tcataaataa ggggaactgt atatgtcata agcctatcac
     8161 ctgcatttgt caataaaaac ttacttcccc agtatcctaa attagaactt gatacatttg
     8221 gattatcaat tgcagctcta aatggtttat aaaaaaaatc atctccatct tgctcatttt
     8281 cacttttaaa tgaaaactta ggttcccaat aactgtccat agatattcca aaacttcttg
     8341 ctatatctga tgaacctctc tcaattaaca aatcagaatt atcatttgga ttaaacttag
     8401 ggtctaaatc ccttatatac aatcctgtcc tacttatttc acctttacta tctattgact
     8461 tatcgttaat atttttgtca cttaggatta taaaagctcc tgtaacagaa ttttttctca
     8521 aaagatatat taaatcattt gatatgcttt ttataatatc tttgttaatt tcttcattgt
     8581 tgcctacatc ctcaattaag gcattttttt catttaatac tttttttaca gaggaattaa
     8641 ttgtagcttc cgtttcactt gtatttgacc atctttgtaa catttcattt tgtatataat
     8701 ttttacgatt aattactctt tcatttaaga tgtcaaacga attatcattt aatttcttaa
     8761 tagttcctcc ccataaaatt gtggcacaaa ataaacttgt ttgaattagc ataacaataa
     8821 ttaggggaaa tatcatcttt ttcattattg aattgttttt tcttagattg ttttccatac
     8881 ccattccacc tatacctttg tgctcgtcaa cacactaact gccaattcat ctattttttt
     8941 atagcatttt caagctcagc cttaaaactt tcaaaccaag ttttaaaatt tttatcatta
     9001 ttaaattgtg ctactgcatc cttcttagaa gttccacttt ttactaattc atttatttta
     9061 tttgaatctt ctttagcttt atcaagcatt gaatattcaa gtatttgtct agctttagtt
     9121 ccaccatcaa atgctttatt agtatataaa ttacaactgt taatctgttc aatagatgag
     9181 gctattgaac cttcaacttt atcagacaaa ctcaaattat ctttcttcat aacttctttt
     9241 ataacatcca aatcattcgt ttttttcttt actggtaagt atccagaagc aatagagaat
     9301 tttatattag gttctttatc tgtaaaccat tttaaaaatt ctactgatgc atattcttct
     9361 tctttagtag attttgttac agccattcct gcaccttgtt gtacagcata ttttccttct
     9421 cctttaaaac gtggtgcttg aaaaacagat gtttcaatat tataactctc attatcattt
     9481 agcattactc tatctggaaa gtatgctgct cctgaactag aacctacaag agctaatata
     9541 tcacctgttt ttgcatcatc agaacgaaac ttaccataag aagcaaaata tccatttata
     9601 tatggtacat agaaattatc ccatagtttt ttcataactt tttcatcaat atttagcgta
     9661 actttaccat ctttaacaga aaaaatttct actcctagtt gtttacttcc tataataata
     9721 taatttgcta aagcatctct tccaaaaaat gctttaccat cattaggctc tttcgttaaa
     9781 ctatctgtcc attcataata tttttttgat gtctctacta atccttcaat tgtttttata
     9841 tcatttatat ttgaatttgt agcttttgca aatttatccc aatcagtttt atttaacatc
     9901 aaaatttcag ttgattttgc aattgggaaa agtttaaact catccttaga atttattctg
     9961 ccttcttcta catattcgtc tatgtattca tttaattctt ctttacttat gtacttatcc
    10021 atatctacta gtaaccctaa tttatccatt tcatatgcag tatctgagta tgctgcaaat
    10081 atatttggta catcttcaga accaactttt ttatttgcag aatctaaaac tttctctgat
    10141 aaatcaacta cattaccttg actgaatgct tcaacaataa ttcctttttc agcacctaca
    10201 gtgtcattaa attccttaac aagcttatca aaagccattt tctgctgtcc attataataa
    10261 tgccaaattt gtattgatgt cggattttct gggtctaact tataattatc ttccttcttt
    10321 aaactacatc ctgtaagtaa taaaacaatc attgatgtga taattaattt tgcaaactta
    10381 cacctattca taaaatctac tccttcaaat tataaaattt ataatagcat taaaactgta
    10441 acttcactat ttatattaat tatagaacgt ttattgatat atttaaacag gatttacaac
    10501 atatttaaaa gcaattaaga aacatactat tacaaatata gaaaataaag cttttacata
    10561 atttttattt ctaaatgcac caaatgacac caatccttgt tgaatagcta ctgtaattac
    10621 taaaagtatc attattatat taagttgaac cagtttaaat aagtaaagca atgaaaacac
    10681 tactagaagt acacataata caatactaga tttattaata atatcatcaa tcttatctac
    10741 tttcttagat tgcttattaa ttttatttaa tttttcttta tttaaattct caactttttt
    10801 attatacctt tctcttgttt tatctttata tttagtattt ttatttttat ttcccattta
    10861 ttttccatcc tctataaatt ttttaattga gcttatacat tatttaaata ttatatattt
    10921 aagataccat atattcctac aaaaaggtaa cttactatgt atctattttt gattttcgca
    10981 taaaaaagcc atctccttta gaattaattc tgtattgaga cagctaattt aactatttat
    11041 cctttgaaac attttctaca ttatcatatt tactatcaac taaaactata tcaactcttc
    11101 tattttttga cctaccttct tcagatgaat tttctgctat tggtctaaac tcaccatatc
    11161 ctacagcaga taatttatct ggtgctatac ctgcattgtc aataagaagt tgaacaacat
    11221 tggttgctct cataactgat aaatcccaat ttgacttaaa ctctgaattt tttatagaca
    11281 tattgtctgt atggccttct actctcatgt aactattcat ttcatttaac atcttaccta
    11341 tttgaataat cttatctcta gaattatcct tcacaatagc tttccctgta tcaaatagta
    11401 ttgtatcttt aaggcttatt actaatcctc tatcttgaac tcttaatgat actgaattag
    11461 ccataccatt ttcgtttaaa tacttctgaa tttcttcacc aattttttta aacttagcat
    11521 tttgtaaagt atctaaatca ttacttcctg gttcaactac tggttctatc ttgctattac
    11581 caccttcaat aactccagaa gaaccaccaa aagcagaatt tagagattga gataattgtt
    11641 tatatttctc cgcatcaaca ttactcatag aatacattat tacaaagaaa atcataagta
    11701 atgttatgag gtctgaataa gttaaaagcc atcgctcatt gttttcttct ttttcatctt
    11761 catcatacaa cataaacatt ccatcctttt atattattca gctctaccac caatttcttc
    11821 aagttcaatt agttcttttg aatttaaaaa tatttttaat ttttctgtca ttaaattaga
    11881 attatcaccc tcaagtatgg ataatattgc ttctatcacc aaaagtcttt cacttacttc
    11941 ttgttgattt atagccttaa gttttgatgc tattggtaac catattaaat tggcagaacc
    12001 aacaccatac aaagtagcca agaatgcaac agaaatttga ggtcctaatg catctggatt
    12061 acttgaaaga ttacctaaaa tatgtactaa tcccataact gtacctatta tccccagtgt
    12121 aggtgcatat ccacctgctg aatcaaaaat tgctattcct tctttatgtc gttttgatgt
    12181 tatctgtatc tgtgattcca atatatttct tatagtaact gcatccactc catctaccat
    12241 aagttcaagc ccttttctaa taaaagggtc taattcatca ttctcactta tttctttgtc
    12301 aatactaaga agcccctctt tcctagcctt tgttgcaatt tctttgaaat agattatatt
    12361 ttctatgtta ttaggctttt tatttttaaa agctaataac attatcttta tagacttttt
    12421 aaacacaggg aatggagtcg aagctccaac agctcctata gttccaccaa acacaacaag
    12481 tgctgatgaa aatacaaata atgctccaac atgtccccca tcaataacaa aagcaactac
    12541 caaagatgct atccccacta tgaaaaaaat taaagttgtc atctattccc tcctttcttt
    12601 agttatacat atctaatcat ttatgacatt ataaaaagac tatctttaat aaaaatagcc
    12661 tttaataatt ggcgg
//
LOCUS       DAYATN010000111        12663 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.111, whole genome shotgun sequence.
ACCESSION   DAYATN010000111 DAYATN010000000
VERSION     DAYATN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12663
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.111"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            322..480
                     /locus_tag="ACGI8A_002823"
     CDS             322..480
                     /locus_tag="ACGI8A_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180813.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(767..1471)
                     /locus_tag="ACGI8A_002824"
     CDS             complement(767..1471)
                     /locus_tag="ACGI8A_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0180814.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1462..3069)
                     /locus_tag="ACGI8A_002825"
     CDS             complement(1462..3069)
                     /locus_tag="ACGI8A_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HFH0180815.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3323..4501)
                     /locus_tag="ACGI8A_002826"
     CDS             complement(3323..4501)
                     /locus_tag="ACGI8A_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180816.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4727..5221)
                     /locus_tag="ACGI8A_002827"
     CDS             complement(4727..5221)
                     /locus_tag="ACGI8A_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFH0180817.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNIGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5222..5983)
                     /locus_tag="ACGI8A_002828"
     CDS             complement(5222..5983)
                     /locus_tag="ACGI8A_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFH0180818.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            6685..9519
                     /locus_tag="ACGI8A_002829"
     CDS             6685..9519
                     /locus_tag="ACGI8A_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0180819.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIMHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            9562..10806
                     /locus_tag="ACGI8A_002830"
     CDS             9562..10806
                     /locus_tag="ACGI8A_002830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFH0180820.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(11221..>12663)
                     /locus_tag="ACGI8A_002831"
     CDS             complement(11221..>12663)
                     /locus_tag="ACGI8A_002831"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180821.1"
                     /translation="LLNHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDII
                     VNVFTAIISLIFLINLQFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSN
                     LIQEYVSNIMDIVILNSKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLIT
                     ILIYGIGGYKIINNKMTMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSV
                     IDEKIDIKVDNNGYRIDNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVG
                     GSGCGKSTITNLIYRLWDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIK
                     NNIVLNNKISDKELDEILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARA
                     LVSNKKVLILDEATSALDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLD
                     KGRVIESGNDAELLHKDGLYNKLVETADFA"
BASE COUNT         4507 a         2050 c         1519 g         4587 t
ORIGIN      
        1 gacaaaaagg gcaatttttt tatatcaaaa aaaaatgtta aattgatatt atccatatat
       61 aaattgatat tatccatata gatgctatat ttaacaattc tacttacttc ccttacactt
      121 attagcatat agttattaag agtttgttgc aaaacaacac ttagcacact ttaaaaagtg
      181 tggtctaata agtaatcaac gaagaagaca taataaagca taaaatatga tatacttttc
      241 cacgccatca aaactgagga ttttattatt aatatatgct aaaatatgct taataaatta
      301 aattttaatg ggagaatagt tatgaaaata tttagtttct tgttgtttgt gataggaata
      361 tgtttatgta ttgctggatt tataggacat tacccagtat ttgttggtac aacattgagt
      421 tttcttgcaa ttgcaatgat agctgtaaga caagtcaaaa ctaagaaaaa aatgaaatag
      481 gcaaatccta actttacaag ttaatattac acaagtaatt agtctaaaat tggatacttt
      541 ttctattaca gaaatacatc atccactttg tattttctta aaaaaataaa atagctatgt
      601 aaattcaaga ttagatagat taactctcta tccatttcat tgtaaaatat acatagctat
      661 ttgtattcga tttaaaatac ttagattttc ttacataaca aataatttct atctgattat
      721 tactcatatt catttagtaa ccactcagca actaatcatt tagtatctat tcatccgcca
      781 tacggtaacc taccccaacc tcagtcaaaa tatattctgg ctgagcagga ttcttttcaa
      841 ttttccttct tatacttgcc ataaaaactc taagcgactg attttcactt tcatagtaat
      901 ttccccaaac tttatctatt ataaacttat gggttaagac tcttcctgaa tacttagaca
      961 tcaactcaat aatcttatat tcgattggag ttaaatgtat ttcttcacca ttaattgaca
     1021 caatatgatt ttcataatct atctttaggt ttttaacttc aaaagacttt ggagcatcat
     1081 tatttatatt atcctgttga gctttatttc taagagccac acgtactcta gccaaaagct
     1141 ctgttacact aaaaggttta gttaaataat cgtctgctcc tccatctaaa gcttccacct
     1201 tatccctctc atgctctctc gctgaaacaa caattatttt attagtcttt gcacatgccc
     1261 taactcgtcc tattacctca atcccatcaa tatctggtag tcctaaatcc aaaattatca
     1321 aatctgggtc aagagaatgt ataagtgata tagctgttcc tccattatct gtctctacac
     1381 acctataatt ttgagtttca agagatactt ttataaattt acaaattggc ttatcatctt
     1441 ctacaattag aactaatggc tttatttcca cttgtctttc ccctcctcat catacatagg
     1501 tatgctaaat ctaaatgtag ctcctccaga aggattatta aaggcgacaa tttctccacc
     1561 atgagcttct actatagatt tacatatctc caatccaaga ccaatacctc ttctttcaag
     1621 atttctagac ttattcttag tataaaacct attaaaaata ttcccgatat cttcatttgg
     1681 tattcctttt ccattatctg caacttcaaa tagtacttga tttgactctt tttttacagt
     1741 tagttctatc ttagaatttt taggtgtatg ccttatagca ttatcaatta gatttactaa
     1801 cacttgttct attaataata catccacatg aacgattatt atctcatctg gaatatttgt
     1861 ttttatgtct ctactattgg caaacttttt gactcttaat atagattcag ctattatttc
     1921 atcaacaact tctggtcgtt tttcaatatc caattttcct tcatctattc tggtcatact
     1981 aagtatattc tcaacagaat gagttagcca actcgtatct tcatatatat ttttcaataa
     2041 ttctactcta gtttctttat ctatcacatc atcattttca agtattgttg aagatgaacc
     2101 taatatactt gtaagtggag ttcttaaatc atgagaaatt gacctaagca agtttcctct
     2161 taatctctct ctctctgcct caagatttgc ttttttattt ttctcaaata aattttctct
     2221 atcaattgcc aacgcaacct gtgttgaaac tgcttctaat agtatctttt cattttctgt
     2281 aataatatct atcttaaaac acgcaacacc tataacacca agaacatttg attcttgacc
     2341 ttttataggg aaataatatg cactgctgtt tttacaatat tttgtcccaa ttccaactgg
     2401 gttacctgtc ctaaaagctt cttttatagc ttctttttct aatactgaat ggaatatatt
     2461 ctcacttcca tcattgttac aaatataaat acttggctta cctagattat tattctcatc
     2521 tgcaatagtc actattacag ttctatttaa tatatttact aaattagacc cacaaaattc
     2581 aataatttga tttttatttt ttgctttaag caacgttttg ttattctcat agagcaatct
     2641 tatcattttt tctctcttat gagaaagtct cacttctctt tttatcctag aagttaaagt
     2701 acttgttata attgctgcca ttagcataat acaaaaggtc ataggataat cttttctata
     2761 agctaaaaaa gtatataatg gatgcgtaaa gaaaaaatta aatgatagca ctcctattac
     2821 tgaagaaagt attccaaaaa aatatccata ggttatactt gtagcaaata gtacacctaa
     2881 tatgtatact agtataatat tagattcatt aaaacctacc ttttcaaaaa ctactgatat
     2941 tccagtagca attgtcataa ccaatatagt ttttatgaag tccttaataa ttatcttttt
     3001 actgagatag ccatcttcat cataatcata ctcttttaag tcttcatgtt gtagtcgttc
     3061 ctttttcatt tactacaatt cctccttatt gttactgttt aaattggctc tattttatat
     3121 aatacaattt tatcataata aattcaatca atacatttaa ttaatgtact taaattacta
     3181 aaaggtgctg tcttaaaatg aaaataaatc tctatttcaa aacagcacct atatatttaa
     3241 attactaatt tagtttttac atacttaaat caatagtttt attcaatttc ataaattata
     3301 atacttcaat tataaaacat ttctatccaa caagcataaa tataaagtgg gcacatatac
     3361 ctgcacataa accacctata gtatgcgaaa gtatcgcttt accagccaat ggtcttgcat
     3421 ctaaagcatc catcatacct atgtgtgtac ttaaatatcc actccaacac atacccattg
     3481 ctgtgaatac tgctatatca ttaggagtta tagcgcctga ttttataaac tctggtacta
     3541 aagatattgc tgctccaaca gctcctaatg cagttacagg gaatgctatt gcctctggtg
     3601 atgtgaatcc aaataatggt tctattatga agctaatctt atcaccaata gcaggtaata
     3661 attttatacc ttcatatgca gctcctgtgt aaacagcttg acctgtagca gggtctgtag
     3721 atggtccaaa agttaataac ataactaatg tacaaactac taaaactcct ggtattatag
     3781 ccatacccat ttcaacacct aacttaccac cttcaagtat agcatccaat gttctttgga
     3841 atacattacc atctcttatt tctctaaact cttcaagttt tgcaccttta tcatttattt
     3901 gcttttcagt atcattttta gggtcataat tataatactt tttagtaaaa gtcaacataa
     3961 ttctaacact aataatacta cctattattg ccgcaacatt acctattata gctggtagta
     4021 catattcttt accttgagct atcataaaag ttgttacaat aagtcccata ccaaaggctg
     4081 ttcctaaatt acataaagca ggtacttgat acttcttaaa atattgtgta aatgttttgt
     4141 ctttagcaaa tggtataata gctggattat cagataaata tgttgcaact actcctgcta
     4201 tactagcacc aggtaagccc catataggtc tcataagacc cttaaatatc ttattaacta
     4261 aagatattac tccaaactct gaaagcaatg cactaagtgc acctgcaaga actgccatag
     4321 ccattattaa aaatactgtg tctaataaaa gcgcatgtgc tgtactcatt attactttaa
     4381 acatcattcc tgcacccatg atgcttccta catatccaaa tcctaccgcc aatagtacaa
     4441 taaatacgaa ggtttccata cttactgcct tgactcttct cttatccgac agcttttcca
     4501 tcttgaaatc ttcccccttt taattttaaa ttaaggttcc taacattggt acaaattttt
     4561 tggtacaaac tgagttctaa aacttacact cgtaatgcaa gttgtcagtt ttttgaaata
     4621 aaaaaaaatt acgtttatag aataagaata tcctctttaa tttggatact cttattctat
     4681 tttatgttat tatattatca tccttttgtt taaaagtcaa ttttttctat ttttgttgtt
     4741 taattttatt tatactcttt ttatattttt cattttcttt tatctttgta agtactccat
     4801 ataagttgac ttttctaaat acagtagtta taaataccaa tcctaatgca attgaacttg
     4861 cacacgataa tgtactcacc ccatattcta cagcttttat ataattattg gtcaaaacag
     4921 aatacatagt attatatatt ccataacctg gtactaatat tattaaacag caaacactta
     4981 atgtagttgc tggagtcata taaattcttg cacataactc acaatatatc gcaaaacaaa
     5041 ctgtagctat aaaaaaacat aagttttctg ttactcctat atttaaaaga aatttatatg
     5101 aaaaccaagc tatacttcca cctattcctg ctagtattaa gttttttcct ttcatattaa
     5161 atagcactcc aaagcttaag gcagtaaaaa aagctgctat tacttctata aacaaatgca
     5221 cttatatccc ccctaacttg aagtacaatc caagtaccaa gaacattcct actgctatag
     5281 atacacctac aaaaaatact tctgctaatt ttgcaagacc agaaatcaac tgtccatcta
     5341 acaaatctct taatgcatta gtcaaagcaa gcccaggaac taagagcatt attgctcctg
     5401 ctattaaatg gtctaacgtc tttatgaatc cgagctttaa aattaaaatt gaacaagaag
     5461 ctataataac tgctccaatt gcatttacaa aaaagtcatt aagggaagat ttacttaaat
     5521 acataataac aaacttagtc agaatcccaa cagtaaatgc agctataaat tcttccacat
     5581 ttcctccaaa taatatcgaa aaagttgctg ctgcaatacc tgcaaaaaat attgttatta
     5641 aatctgagta atagttatct tcacttattt catctaaaat tttctcacat aattcaatat
     5701 ctaaattatt tatacttatt gctcttgata atgagttaac ttcatgaacc aagtttaggt
     5761 ctactcttct tgagcttatt cttttaattg ctgaatgtaa tttcccatct acaaatacag
     5821 atgccattac agctgttgga gttgcaaata catctacgtg gtctattcca aaagattgac
     5881 atattctagc tatcgtttct tcaactctat aggtttctcc tccactttgc aacatagctt
     5941 tacctgcatt tgaggaaaag tttaaaattc tatctatatc cacaaaacat atctcccatt
     6001 cttttatagt attattcata ttttaataca agatattatt tatttaaact attttaaata
     6061 attcatatcc aatatttaca ttttatgaaa aaacttgttt tttatgcttg tcaaaatcaa
     6121 attttaagct tgtttacacc ataaaatggc tgaattaata tttgacaaac actttcaacc
     6181 atttatataa ccttctaaat tgtataactt tttactacaa ataaaattat atctctttta
     6241 attctttctc ttgagtattt agcttatagt aacttcatat ttatatatgt acaatataaa
     6301 acattatttt tatgatacaa aatataattt gtaaatgtat atatgtatta actgtgtata
     6361 tataaattat tcttgacctt atcagttttt tcatatatct tcatctatac caaccatata
     6421 ttaataaatg atattacata gacttaagta tattaaaaaa cttaaattta gttatttaac
     6481 aacataatac tctaatttaa tctataaaaa tccatactta atataatgca actcttattt
     6541 taacaccatc tattacacaa aacaacatat taaaaagtaa aatacaaaat atccactata
     6601 aaccttattt taatactgaa tatatattat aatatattta atgtagctta aatatagttt
     6661 aaacataaag aaaggaaacg gtatatgcta agaaaagata aagttctatt atctctacat
     6721 aaattatgtg aaaatatgac tttaaaagac cttcaatcag ataaaacagg attcactaca
     6781 atagaaattg ctaaccattc atctcttgat agaagtaata ccagtaaaga attaaataac
     6841 ctatacaatg aaaaaaaagt tctgaaaata tctggtaagc caattttatt tctacatgct
     6901 agtattatag aaaatttaat tggccgtact ttgaacagtg atgaattttc tataaataat
     6961 tgtaatgagc tacttaaatc aagtaaaaat gactcagaag atattttttc aacattactt
     7021 ggacatgatt ctagtcttaa actacctata gaaaaagcta aatctgctat actctatcct
     7081 ccaaaaggtc ttaatacgtt actaatagga cctactggtg ttggaaaaag tacatttgct
     7141 gaaacaatgt acaaatatgc tgtagaaagt aaaatctttt cttctgaaag caaattcgta
     7201 gtatttaact gtgctgaata tgcagaaaat gctaacttat tattaagtaa tttatttggt
     7261 tatgtcaaag gttcttttac tggtgctaat aaggataaat tcggcatagt tgcacaggct
     7321 gatggaggca tattattctt agatgaaata cacagattgc caccagaagg tcaagagatg
     7381 ctttttcttt tgatggacaa aggcatatac agacgattag gagaatctga cattatgcac
     7441 aaagctaatg tctttattgt gtgtgccaca acagaagata tacattcttc tctgcttggg
     7501 acttttttaa gaagaatccc tgttacaatc aacctacctg ctttacagga taggtcttta
     7561 aaagaaagat tatctcttat taaatacttc tttattatag aatcaaaata caccaatgca
     7621 actataaaag ttcacaaaga tgtaattaaa tcattactcc tttatgattg ttttggtaat
     7681 ataggtcagc ttaaatctga catacaactt atgagtgcaa gagccttctt aaattacaaa
     7741 actggactta aggataatat agaaatagat ttatcccttg ttcctgatta catctatatt
     7801 ggtcttttaa aattgaatga aaatcgtagt aatctaaata aattatcaga acttgattat
     7861 atgctttttt atgaatttaa tggtaataac ttagaagttg aatatacaaa agaactatct
     7921 aaatacaata tagatacatc aaacaatgga tttaaatact ctacagagat tccttatctg
     7981 gaaaatgata taaggagtat gtttataaat tacatggaaa agtactctac aaaattactt
     8041 tcgatatctt cagtaactca ttctgattat acaagtaatg agatttttaa agtaatcaat
     8101 ccaaagattt actatgctgt tgaacattct ttaaaagagg ctgaaaaaat atcgagaaac
     8161 aaatacacaa aacaaactta tattgctctt tcaatgcata taagtgcact tatagaaaat
     8221 ataaatcaag acacatataa tcctaagtac aatttatata atgataaaat tttcttagat
     8281 aaagatttaa atatagctaa aatagtttta agtataattg aaaatgactt acaaacatct
     8341 ttacctaaag atgaagtaaa atttatatcc atgtttctaa atagcatgta tcttgaacca
     8401 aacaatcaat ctgaaagtat aggagtcata atacttgctc atggaaattc aacagcaagc
     8461 agtatgtgtg atgtagcaaa ttcattgctt ggtacaaacc attgtaatgc aattgacatg
     8521 cctcttgatg taaaagtaag taccatactt gataaaacta tagagttggt aaaagaatgt
     8581 gacgaaggta agggtgtact tcttttaaca gatatgggct ctcttatagc ttttggagaa
     8641 cttatttcag aaaaaacaaa aatcaaaact cgctcaattg aaatggtatc tactccaata
     8701 gttttagaag cagtaagaaa aagtacacta ccagaaatga ctttggatga attagctaat
     8761 tccttaagct ctttaaatcc atatattgga cgagcagcaa tcgattcaac agaaataaac
     8821 tatcctgaaa agtcaagaga gtatgtaata ataactactt gtataacagg atatggttca
     8881 gctatgaaag tagcagactt tataaattct tcacttaaaa atattagtaa atacaatatt
     8941 aatttagttc ctcataatat agaaagtttt aaaatgtata agaataaatt tagtaatact
     9001 aaaattctgg cagttgttgg aagtgtagat tttaatgtag ccaacacccc ttttatacac
     9061 atagaggaac tactaaatgg atatggccta gatatgctaa acaaaataat agaaggtaca
     9121 ttcaatgcca atagtattac aaatataaaa gaaacaaatc tatcatacaa cagtttaata
     9181 ataaacttta tgacccaaaa cttagagctt ttggactctg ttaaattatt caactatgtc
     9241 aattcttcct tatttacaat aatagaatta atagatgaaa acttaaagag caaatttaag
     9301 ctaggatata tactacattg ctcttttatg atagaaagat gttttaaaaa tacaactttt
     9361 ccatataata acattactga attaattagt aaaaatcatg aactatatag tttaatacgt
     9421 gaagccttta agccaacaga agcttatttt aatattttga taccagacga agaaattgct
     9481 tatataatag acatgattaa tgagtatcta aataaataaa atttatagca cttattttgt
     9541 gcatattaat taatatatga aatggaggac gagattttga aaggaaaaat aaaacagtta
     9601 acaatacttg cattaatttt tatatttata actcctgtat ttgcatttgc agatactcca
     9661 ccagtaccaa attcttcaag agcagcacta cttatagacc aggaaactaa aagaatatta
     9721 tttgaaaaaa acattgatga aaaaatgcca ttagcgagtt taagtaaaat gatgaccttt
     9781 ttattggcaa tagaagcagt tgataaaaat caagttaaag agactgatat ggtgaaaata
     9841 gacaagtcta cagcttcagt aggaggttct acttgtaaac ttaaagatgg tgatgaaata
     9901 tctctaggag agcttatgca gggtctaatg ctagtatctg gaaatgatgc tgctatagcc
     9961 atagctaaac atattggtaa aactgaaaag aattttgtaa atatgatgaa caaaaaagca
    10021 gaagaaatag gaatgattga tacgtattat tttaatccaa atgggcttcc tatatatact
    10081 gacccagaac ataaagaacc tcctattgaa aatatgtcaa ctgctcacga tatagttaca
    10141 cttggaaagt atatgtacga ccattatgaa aatcaagtta caagaataac cactatgcaa
    10201 gtatataatg atactaaaaa ggattttaca cattataaca caaatcctct gcttgtttca
    10261 gtacctggtg ttgatggaat aaagacagga tatacagata atgctggata ttgccttgca
    10321 ttttctatga tggtacctaa agatgctaaa aacgaaagaa atcatagatt gataggtgtt
    10381 gtacttggtg atggtaacaa gaaaaataga atttcttctt ccgctacttt attgaaatat
    10441 ggtaaagata atttccattc taaaaagata gcacataagg gtgatattat agagactcct
    10501 tgtgttgatg gtattgatga ttttaagata acagttaagg ttgataaaga tttgtatggt
    10561 gttgtttcag ataacgaaaa tatcaatcct aaggtagtgt ttaaaaatat gaattatcct
    10621 atccataagg gagatattgt tggtgttgca aagtattata atgactctgg taaatttgtt
    10681 ggaagtgttg atgtaaaaag tgaaagtaat ataggatgta ttcctttgaa ggataagatt
    10741 aagattaagg ttgctaagat aaataaaaaa cttgagatta aaaattctgt ttgttttaaa
    10801 gcatagtaaa gcatattatt aaaagggtat gttaaattta taaagactcc actttgtgga
    10861 gtctttttgt tttttacaaa aaattaaaat agtggcaaca attgaggatg tgagggagag
    10921 ttgccactat tttgtatagg gtattatatt atgtttttaa gctagtttta ctatcttcta
    10981 tgtttatata ataactcttg tgtgtgaatt taattgataa ttaatatgaa aattatgtga
    11041 agttttgatt tatttgtaat atattctact actattataa tttttctact ggatatacta
    11101 atcatttgtt taacattatc tgagattgat ttgcaaactt ttatataact aaattagtta
    11161 tataacttat agattccatt aaatacccta ataatcatat atgtacatac aaagtcatgc
    11221 ttatgcaaaa tctgcagttt caactaactt attatataaa ccatctttat gtagaagttc
    11281 agcatcattt ccagattcta ttactctacc tttatctaat acatatatat tatcagcact
    11341 cttaattgtt gaaagtctat gagctattat aataactatc ttatcttgta tgtactcttt
    11401 caagttgttc aatatctctt tttgtgaaat attatctaga gctgaagttg cttcgtccag
    11461 aataagtacc tttttattac ttactaaagc tctagctatt gatatacgtt gtttttgacc
    11521 tcctgatatt ttgacacctt tttctcctac tacttcgtcc aatcccttat ctagattttt
    11581 tataaaatca tctaaacaag taacttctaa tatctcatct aattctttat cacttatttt
    11641 attatttaat actatattat tttttattgt gtcatcaaat acaaattgat cttgagttac
    11701 tatatcaata ttatttctta gtgattttaa attaatatcc tttatattaa caccatctat
    11761 taatatatca ccttcattta tatcccaaag tcggtatatt agatttgtta ttgttgattt
    11821 gccacatcca cttccaccaa caattcctgt tacttcccct ttctcaaatt gtaaatttat
    11881 atcatttaaa acaaggttta aatcctcacc atatctaaaa gatacatttt taaaatctat
    11941 tttcttaatt tcactgttat ctattctata tccattatta tcaactttaa tatctatctt
    12001 ttcatctatt actgaaaata ctctattgat agacactttt gattgtgcta ttctagtatt
    12061 cgcccttact atagctatac atggtccaat taacattcct gtatattgtt gaaatgctat
    12121 taactccccc atcgtcatct tattatttat tattttatat cccccaattc catatattaa
    12181 tattgttatc aatcctgtta gtatttgaga tattgctata ttactactca taatgatgtt
    12241 caaattcaaa tcttttttta tcaaatctct ctctctcttt aaatacttat taaagaaatt
    12301 cttttttgaa tttaatataa caatatccat aatattagat acgtactctt gaatcaaatt
    12361 tgataaactg cctgaatcac ttcttatttc agaaattttc ttagatataa gttttgtcat
    12421 tttattttga gaaaatgtta atatcaactg taaaaataaa actactaaaa ataaatcaaa
    12481 ctgcaaatta attaagaaaa ttaacgatat tatagctgta aacacattta caataatatc
    12541 aaatataatc tctgctccaa agttttctat cataaatata tcactttcca taatggataa
    12601 aatattacct gttttcttac ttgaataata tctaccagaa agttttgata tatggttaag
    12661 caa
//
LOCUS       DAYATN010000112        12646 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.112, whole genome shotgun sequence.
ACCESSION   DAYATN010000112 DAYATN010000000
VERSION     DAYATN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.112"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            950..2308
                     /locus_tag="ACGI8A_002832"
     CDS             950..2308
                     /locus_tag="ACGI8A_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFH0180822.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2415..3209
                     /locus_tag="ACGI8A_002833"
     CDS             2415..3209
                     /locus_tag="ACGI8A_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFH0180823.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3202..5508
                     /locus_tag="ACGI8A_002834"
     CDS             3202..5508
                     /locus_tag="ACGI8A_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFH0180824.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKILSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5647..6924
                     /locus_tag="ACGI8A_002835"
     CDS             5647..6924
                     /locus_tag="ACGI8A_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFH0180825.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASFNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7107..7829
                     /gene="yqeC"
                     /locus_tag="ACGI8A_002836"
     CDS             7107..7829
                     /gene="yqeC"
                     /locus_tag="ACGI8A_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFH0180826.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRNKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHRTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            7936..8505
                     /gene="mocA"
                     /locus_tag="ACGI8A_002837"
     CDS             7936..8505
                     /gene="mocA"
                     /locus_tag="ACGI8A_002837"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFH0180827.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8502..9521
                     /locus_tag="ACGI8A_002838"
     CDS             8502..9521
                     /locus_tag="ACGI8A_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HFH0180828.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            9859..11484
                     /locus_tag="ACGI8A_002839"
     CDS             9859..11484
                     /locus_tag="ACGI8A_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFH0180829.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            11683..12159
                     /locus_tag="ACGI8A_002840"
     CDS             11683..12159
                     /locus_tag="ACGI8A_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180830.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(12367..>12646)
                     /locus_tag="ACGI8A_002841"
     CDS             complement(12367..>12646)
                     /locus_tag="ACGI8A_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFH0180831.1"
                     /translation="FFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLI
                     NSKVPIKVISERVGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
BASE COUNT         5013 a         1304 c         2337 g         3992 t
ORIGIN      
        1 gtatatttta tgttgcagat acaaatatag taaaggagag tacattggta caactgagat
       61 acatagctta ttaagttttt ctgtcttatg agaattatat tataaaatat tcataaatat
      121 tttgagttta aattaaataa ttattttatg cttagattaa ttgatattta attaatttaa
      181 taacaaaaac agtgaaagag taaataatat tatttacagg ggataaagca aagtatgtta
      241 aataaattgt acttttggcc ccgtacatag agaaatctat gtaggacaca actttaaaac
      301 cagtaaatcc taaagccttg caactacaat aaaagataga aagaaacttt tatgaatgtg
      361 gtgaaaacca gaaacaatag tgaggatggt atatggtgag agcctaagta cttaaaaatg
      421 gacatttggt cgccaagcct agaaatagga aggtcaaacg actatctcga gagagagtac
      481 actataagcg attgatagtg gaaacgggtt gctcttagca ggtaatgctg aagatgaaga
      541 aatagtctac gctcatatga aagtatgagg tcactggatt tgaagtgaca tctataaagt
      601 agcgtttata ggtaaatgtt taaaacttga aacatgtaaa tacttatgaa aacttataac
      661 ttataatgat taagattgaa agatttaaat tgaattaaat ttactataaa aaattaaaaa
      721 ttcatagcta taatactctt atatttgtaa agcatggtat ataatgtata caaaattaaa
      781 gaatacgtgt atttgttaat aattaaattt attgcatctg ttttttaaga ctaaaaactt
      841 aaatatatgt tggagtatag ttgatattta tgaacatcat taatttaata tattagattt
      901 ttaagtattt atataattca ataagtttta tgtaacggga gtttttatta tgaaagcaaa
      961 aagtacatca aaatttgaat tagacggaat accaccacta aaggaagcaa tacctttagg
     1021 gttacaacac ttaatggcaa tggtaatagg tagttgttta ccagcgatat tgattgcaaa
     1081 tgcagctgga cttaatcata agatgtcaac attattgatt caagccgcat tagtttttgc
     1141 aggattatca actttattac aattatatcc aatacctttg tttaaaggtt ttaaattagg
     1201 aggaggaata ccagttataa tgggagctac agctatgttt ataggagctg gggtatctgt
     1261 tgcaagtaag tatggactac cagtattatt tggctctcaa ataatagctg gtatagtaat
     1321 aattataatt ggttttggtc ttaaaaaaat acgatttata tttactccag ctgtatcagg
     1381 aacaatagtt gcatgtatgg gtcttggact ttttccaata gctataaaaa acttagctgg
     1441 gggtatgggc aatgagactt ttgggaatcc aattaatttt atagttggaa tatctgtatc
     1501 tcttatgata ttaggattaa ataagtatgg aaaaggatta tttaaagatg catcaatatt
     1561 agttagcata atttttggat acattttatc attaattttg ggtatagtaa atttttcttc
     1621 aattcaggag tttactttgg ttgcacttcc aaaaccactt gcttttgggc tagatataag
     1681 acttgaagtt gttgtaatgt tttcaataat atatttagtt gagatagctg atataatggg
     1741 tgcttgtaca ctttctgcgg taggagggct taatagacaa gtaacagatg aagaactttc
     1801 ttctgctgtt ttaggaaatg gagtactctc tacaataggt gcattatttt ctgcaactcc
     1861 aatggggatg tttggacaaa attctgcaat cgtttccaat acaaaggttg taagtaaatt
     1921 tgtactagct attggaggaa ttggattatt tttggcagga atatcgcctt tattagctaa
     1981 tttaataaga acaattcctc cctgtgtggt tggaggagca acactcgtta tattttcaac
     2041 tcttacaaca tctggtttaa gacttgttag tatggatgga tttaatcaag aaaatagcat
     2101 gatattaggt ctttctatgg cttctggtat tggattcatg gtagctcctc aagttcttga
     2161 aaaatttcca aaatttatag agacattact tgcagattca agtgtagttt caggagcaat
     2221 ggtagccata ataattcaag ctttatatat ggtaaaattt cctggaaata aaagtgataa
     2281 ggttgaggat aaaaataaaa gtttataatg taaaggttta atatatgtga attaaatata
     2341 ctacaattat aaatataaat aaaagattta ttaaaaactt ttgtgtttta ataagttaat
     2401 gatgggggga taagatggtt actataaagg aatattcagt tccacagagt ttagaagaag
     2461 catataaaat tttaatttct aaaaaaaata atgttatttt aggtggatgt ggatttataa
     2521 aattaagtaa taaaaatata ggaacagcga tagatttaaa agatataaat ctaaattata
     2581 taaaagagga taaaaaaaat attttgatag gtgcagatac ttcacttaga agtttagaaa
     2641 ttgataaaac tataaaaaat tactgtagtg gaatactttc aagcgctgtt tctaatatag
     2701 ttggtgttca atttagacaa ggggctagaa ttggtgcaag tgtattttca aagtatggct
     2761 tttctgactt aataccagca cttttggttg ttggagcgaa ggtgagatta tacaataaag
     2821 gaatactaga gttaagtgag tttttggata gtgaattaag tagggatata ttggtcgaag
     2881 taatactacc aaaaaaagat gcaatagctg tttttgactc aataagaaaa tgcacaggag
     2941 atttttctgt attaaattca gcaatgctaa aagaaaatga tacttacaaa atagcaattg
     3001 gggcaagacc tcaaaaagct agattagcac ttgaagcgag taatatatta aataaagaaa
     3061 aagatataga taaggcatct attgttgcta gtaaggaact tacttatgga agtaatatga
     3121 gagctagtaa agagtacaga aaggatatgg caagtgcatt agttaaaagg atgtataatg
     3181 ctattgagga gggaatgtat aatgattaat aaggggataa gaaagataga tagtacagca
     3241 attgtcacag gaaaacctct atatactgaa gacctcatta tgcataaaga tgttttgaca
     3301 ttaaaactac ttagaagtcc acatgctcat gctaagataa agaatataga tacatcaaaa
     3361 gccgaaaaaa ttccaggggt tgtgggtatt tatacgtata aagatgtacc acaggtcaga
     3421 tattgtgttc caggtcaaac agaaccagaa gcatcacctt atgatagagt tttacttgaa
     3481 gatacagtta gatttattgg agatgaagta gctttagtgg ctgctgaaga tgagaaaaca
     3541 gcacttaaag ccttaaaaat gataaaggta gattatgagg ttttaaagcc tgtgtttgat
     3601 gcaagagaag cagaaggaaa tgaagttgtt atacatccag aaaaagacat agtagagcca
     3661 tacccttttg gattggatgc aaaaaataat atagtatcaa aagaattttt tgaatttgga
     3721 gatgttgaag ctaagcttaa agaaagtgat tttgtggttg aatcttcata caaaacccaa
     3781 agtcaatcgc attgcatgat ggaaactcat aggtcatatt catataaaga tataaatgat
     3841 agaattgttg tagtttcatc attacaatca ccacacaatg taaagagaat acttagaaaa
     3901 gtattgggta taccgtcttc aaaaataaga gtaattaaac ctagaatagg tggtggattt
     3961 ggaggaaaga atatagcagc aacagatata tttactgcat ttgttactat gaaaactggt
     4021 agaccagcaa aatttgtatt tacaagaagt gaaacttttg taggttctaa tagtagacat
     4081 gaaatgtttt ttgatgttaa agttggtgca gataaagaag gaaatttaaa agcaatagag
     4141 ctaaagtcat taaacaatac aggtgcttat ggtgggaatg gtgtctctgt ttcttccgaa
     4201 tctggacaca ataccttacc aatatataat gatattgatg ctgttagatt taatgctaga
     4261 acagtatata ctaataggtt accagcagga gcacttagag ggtatggtgc tacacaggga
     4321 atatttgcaa tagatagtgc tattgatgaa ttggcacata agattggaat ggacccactt
     4381 gaatttagaa ttaaaaatat aatagatagg tataccaatg gaaaagcagc tgaagacgaa
     4441 atattaagtt gtgagctgag acaatgtata gaaaagggaa agaaattaat taattgggat
     4501 gataaatatc catgtaaaga tataggaaac aacaaagtaa gagcagtagg tatggcagta
     4561 gcaaatcatg gttctggtat accgcgtgta gatatggcaa cagtcactat aagaatggat
     4621 gaagatggca catataaaat attatctggt cttgctgact taggtcaagg ggctgataca
     4681 attcaagctc aaattgctgc ggaagcttta aatactactg tagatagaat ttctatttat
     4741 acaggtgatt cagacttatg tccctatgat acaggagcat ttgcttcttg tacgacaacg
     4801 gttacaggaa atgcaacaat aaagacagca aataaattaa aagatatatt actttcagtt
     4861 gcagagtcaa agctaggatt taaaaaagaa gatttagagt tgggaaaaga cagggttttt
     4921 gttaaaaacg ataatagcaa gtttgttcta ttagaggaat tgggtagaga atctgttgga
     4981 ggaataggat ttgctagtga tgatgtacca gtagttttgg aagcaacaga atcttttggc
     5041 atgttgaaaa aaataaaacc atttatagca ggatttgcag agatagaatt agataaaaat
     5101 acaggtgaat ataaagtcat aaattttgca tgtgtacctg attgtggaac agttataaat
     5161 ccaactttag ctagaataca agtagaaggt ggtgtgatga tgggtatagg gtttgcaatg
     5221 tatgaagacc ctatatttga taaagatggt agattattaa cagatagttt cctacagtat
     5281 aaagttccta caaaaaaaga agttggaaat atattggtag attttgctga gaatttcgac
     5341 ccaggaggtc cttatggtgc taaatcatta ggagaaatag ttatacatac accagcacca
     5401 gctatagcaa atgcaatatt taatgctaca ggagttcgtt taagagagct tcctatgact
     5461 tttgaaaaag tttatatggg tatgaaaaat aataataaac aattataata aataataaga
     5521 tgcataaaag caagtaaatt taaattattt tgtaaaataa agtaattaaa tggacattat
     5581 atatggtgtt ttgtaaaaat accgtatata atgtctagta gagtatattc taaaggaggt
     5641 gagttcatgt caaacagaat aaaaaatata cactatggaa ctcagagaca acaattaaaa
     5701 aacttaacta aaaaagaata tcaagctcta cgagagatgt gcttcttagc taagaactta
     5761 tataatgttg gtacatataa tgtaagacag cattatttca atcaaggaga acatcttaac
     5821 tatgaagcta attatcatgt atgcaaagaa aatgagaact ataagcttct caatagtaat
     5881 atagcgcaac aaatattaaa agaagtagac agttcgttta ggtcattttt tggattaatt
     5941 aaattagcca aagaaggaaa atatgacttt agagggataa aatcacctaa atatttagat
     6001 aaagaaggat tttttagcat aataataggt caaataagaa tcaaagaaga tgggatttta
     6061 aatgtaccta tgtctccagc atttaaaaaa ctatatggga aagtaagtat aaaagttcca
     6121 caaaatatac tagagaaaaa aattaaagaa ataagaataa tacctaaaca taaagctagg
     6181 ttctttgaaa ttcagtattg ttatgaaata ccaaaatcag aggaagtttc aaatcaaaaa
     6241 aatgcactag ctatagactt gggtttagaa aatttatgta catgtgttac caatttaggt
     6301 gatagtttta tcatagatgg aaaaaggcta aaatctgtga atcagtgggc aaacaaacag
     6361 aatgataaaa tagaagcctt aaaattaaaa aagaatataa ctgcaataac aagaaaacaa
     6421 tttaaagttt ggaataagag aaataatcaa gttaaagact atgtaaataa aacctgtttt
     6481 tatataattg attattgtgt aaaaaataat attggaaatt taattgtagg atataatgct
     6541 aagttagaaa aaagtgataa tatggagaga aaaatatata gaaattttat aagaatccca
     6601 tttggagaga taaaaggaaa attagaatat ctgtgtaaaa taaaaaatat caattttatt
     6661 aggcaagaag aaagctatac tagtaaatca gatttttttg ctgatgattt tctacctgat
     6721 atcaatgtgg acaatgttaa aaaatatgac tttaaagggg aaagaataac acgtggacag
     6781 tacaaatcca gtgttggaat cattataaat gcagatgtaa atgcgagttt taatatacta
     6841 aaaaaatcac aagtagttaa tttatctaat ttacagaata atcctcaaat attaaagcaa
     6901 ccacagagag taagaatatc ttaattatat ttaaaagata aactgatagc gtaagcttac
     6961 agcttagcgg cgaattggat ccgccttgtt gtttacaaca gaatctattt actttcgagt
     7021 ttatggaatt tcaaaaatca cttttactaa aaaggcctaa ctataataat taagagatta
     7081 gaaaactggt tataagaggt gaaagtgtgg aactatcaaa tataatagaa aagaatgaaa
     7141 taataactgt agttggagca ggaggaaaaa cttcgtttat aaactatttt gcaaattttt
     7201 atagaaataa attgaaagtt cttcttacaa caacaactaa aatatatgta ccaaacgatt
     7261 atgacaatat aattattaca atagatggaa ctgttattcc atctatttgt catggaataa
     7321 cagtctgtgg cagttatata aataatgaaa ataaattagt aagtattgat tcctctattt
     7381 tggatgaaat tgtagaccaa tttgatttag ttttaataga gggagatggc tcaaagagaa
     7441 aaaaacttaa aggttggaat gctaaagaac cagtagtata tcatagaact acaaaaacca
     7501 tagggatatt agatataaca tcatttggaa tgaatattaa tgaagagaat atacatagag
     7561 tagaaatatt taaaaaaata gcaaatttag atacaagtag tattaatagt agttctacag
     7621 taagtattga aaacttaaaa aatatagtat taaatccaaa tggattattt aaaaattatt
     7681 ctggaaaaag agtattattt attaataaag ttgaaaatga aaaatataaa aatttagcta
     7741 taaaattaat agaaaatatt aaagaatatg aaagtgaaat agagatattt tatggaagtg
     7801 taaaacaaaa attttgtgta agatattgaa gtattaaaat agtttatatg atattatata
     7861 tatagataat atcaatagag atattataaa aatcatagaa ctatcaatat attataaata
     7921 ccaaggatga tatatatgat aaatgcagtg ataatggcat caggctttgc cagaagaatg
     7981 ggagcaaata aacttttatt aaattataaa ggctgttcta taatagaaca tgtatttaga
     8041 gaagttagca agattgattt tcatcaagtt gtagtagtaa gtcaatatga agaggttttg
     8101 ttattgtgta ataaatatgg ttttgaatat atagataata aaaatgcaaa tataggtcaa
     8161 agtgaatcta taaaattagg agttttaaat agctcaaaat gtgatggtta tatgttcttt
     8221 gtaggagacc agccatttat tgataactta tatatagata aaattataaa tacttttaaa
     8281 ttagatagag attttatagt aattccaaga tacaaagata tgtgtggaaa tccagtcata
     8341 ttcccatata gcaagaaaga agaattgttg gcacttaaag aagatgaaaa aggtaaaacc
     8401 attttaaaaa attcatcaaa aataaagtat gtagaagttc ctaaagaaat gttatttgat
     8461 atcgatacaa aaatggacta tgagagaatt gggggtaaat tatgaagcta ataaaaacta
     8521 tagatgctgt tggacaagtt ttatgtcacg acataactca aataatacct ggagaattta
     8581 aaggaagaaa atttaaaaag ggtcacatag taaaagaaga agatattcca gtgttattat
     8641 ctcttggtaa agataatttg tatgtatggg aaaagtcaga aggaatggtt catgaaaatg
     8701 aaggcgcatt gtttttaaaa gaactaactg ctggagaaaa tttagatttt agtgaaataa
     8761 aagaagggaa aatagatttt atagcagcat gtgatggtct attaaagata gatgttgatg
     8821 cactttttga tttgaattgt gttggagaaa taatgatggc tactcttcat aataatttta
     8881 tagtaaataa aggtttaaaa gtagctggaa caagagtgat acctttagtt atagatgaga
     8941 aaaaattaga agatgcaaaa aaagtagttg gtaatagaaa aatagtaaat gtagtacctt
     9001 ttaaacctaa gaaagtagga attgttacta caggaaatga agtgttttat agcagaattg
     9061 ttgataaatt tggaccagta atagaagaaa aagtaaaagg ctttggttgt gaagttatag
     9121 gacagactat atgcccagat gataaagaaa ttataaagag tgcaataaaa gaatttataa
     9181 gtcaaggagc agaacttatc tgttgtactg gtgggatgag tgtagaccca gatgatgtta
     9241 cgccaacagc tataaaagaa actggagctg atttagtgac atatggttca ccaatattac
     9301 caggagctat gtttttactt gcttattacg gagaagttcc aattatgggt ataccaggat
     9361 gcgcaatgta tcacaaaact acagtatttg atatagtttt atctagggtt ttaattgatg
     9421 aaaagcttga taagtatgat atagctagat atggtcatgg tggcctttgt atgaactgtg
     9481 atgtatgtac ttatcctgca tgtaattttg ctaaaatata aagttattaa aaaagagtat
     9541 ttacaaatta agtactcttt ttaaatatga aacaaaacag ctagaatgat gttttaaaaa
     9601 aagcatattt atttagaaat tataataact tattttcaat gtgtaaaaga atgaataaag
     9661 ttgcaatttg atttctttat aagtataata taagagattg ttaattttta taaatttaca
     9721 atttttagta ttattttaat aaatatttca caaatttagt ataaaaaata tactatttac
     9781 gtgataatat ataataaaaa tgttatcaaa gttttatgat aaaataaatt atgagcaaaa
     9841 agaaaggaaa atatattaat gttacaagtt acaggtgttg gacttagatt tggtgataag
     9901 gaactattta aagatgttaa cttaaaattc actaaaggta attgctatgg aataatagga
     9961 gctaatggtg cgggaaaatc tactttttta aaaatattat caggagaaat agagcctaat
    10021 acaggtagta tatctataac tgataaagaa agaatgtcag ttttaaaaca ggaccacttt
    10081 gaatatgaag aagaaactgt tttaaatgtt gttataagag gtcatgagag actttggaac
    10141 ataatgaaag aaaaagatgc attgtatatg aaggaagact ttaatgaaga agatgggata
    10201 aaagctgccg aacttgaagg tgagtttgca gagcttgacg gatgggatgc agaaactaat
    10261 gctgagaaaa tacttatggg tcttggcata acgaaagata tgcattacaa acaaatgaag
    10321 gaactagttg gtggggaaaa ggttaaggta ttgttagcac aatcactttt tggtaagcct
    10381 gaaatactat taatggacga acctacaaac cacttagatt ttaaatcaat aaactggtta
    10441 aataatttta taatggattt agaagaatct atagttataa tagtatcaca tgatagacat
    10501 ttcctaaatc aaatatgtac taatatagtt gacgtagatt ttggtaaaat acaaatgtac
    10561 gttggaaact atgatttctg gtatgagtca agtcaattgg ctttacaatt ggctaaggac
    10621 caaaataaaa agactgaaga aaagatagct caattaaagg aatttatagc tagatttagt
    10681 tctaatgctt caaaggctaa acaagcaaca tcaagaaaga aacagttaga gaaattagaa
    10741 gttgaagata tacaaccatc aagaagaaga tatccatatg taggatttac acctgcaaga
    10801 gaaattggga atgaagtttt agaagtacac aatttaacta aaactataga tggggttaag
    10861 gttcttgata atgtgtcatt tagattggat agagatgata aagtagtatt tatgggagat
    10921 gaaatagcta ctactgcact tttaaacata gttatgggag aacttgaacc agacagtgga
    10981 gagtataaat ggggagtaac tacttctcaa gattatcttc caaaaaatca taataagttt
    11041 tttgacggag tagaatattc tttggttgac tggcttagac agttctctga agaaaaaagt
    11101 gaaagtttta taagaggatt tttaggtaga atgttattct caggtgaaga agcattaaaa
    11161 gaagctcaag ttatatctgg aggagaaaag gttagatgta tgcttagtaa gctaatgctt
    11221 tcaaatgcaa atgtacttgt attagatgac cctactaacc acttagacct agaaagtata
    11281 acttctgtaa ataaaggact tgaaaagttc ccaggagtgc ttttatttac ttctcatgac
    11341 catgaattta tatctactat agcaaataga attatagaga taactccaaa tggaataatg
    11401 gatagaaaaa tggactttga tgagtattta gaaagtaaag agatacaaga ccaattagct
    11461 aaaatgtatg gaaaagataa ataattatta gtataaaacc ttttaatatt tgagacatgc
    11521 tatgcatgag tgtgtaaatt tttaatcaaa tattaggagg ttttttatat tgtttaaaca
    11581 tgtttattag cagaatagag taatattttg tatattaaga atttaaatat aatatagtag
    11641 atgatataat aaaaatatat aacattttgg ggggaataga ttatggttaa gaaaaggtta
    11701 gtagctgtag taattagttt agctatgatt agtgtaggga atttttcata tgctcactca
    11761 ggagggcata attcatcagg aggtcatcat aataaaaata aatcagagtt aacacgttat
    11821 tattgtggag gacattcagc acatcaacac aataaaggtt tttgtcctta tgctagtaca
    11881 aaagaaaatt ctggagtaac aatatcttca gtacataacg aaaaaaatca taaaaactat
    11941 tttgcagaaa aagggtataa taaaggttat gaagatgggt gtaaagggct ttataattta
    12001 agtgtcacag aagccaaatc tgataattta tatgaaaaag agattttaaa aagttcatat
    12061 gaaaaaggat atttaattgg atacaatgaa tataaagcaa aaaaagtaga tgatgtaatg
    12121 aagaagatgt tagatattcc aatgttagca agtatataat aatatagtat ttaaaaagat
    12181 attttctata agggaatatc ttttttattg taaagtataa tttttcgtaa aaaagtataa
    12241 taactgaggc gataaattaa aataaaacag attatttaag tagttattaa tttgagatag
    12301 atacatttaa tattaatgaa tctatctcaa taagctaagc taatttaaag ctaagataaa
    12361 ttaatattaa ttagcattaa ataaactttc agaaattttg tcagaagcct ctttatccat
    12421 ttctttaagt acatgagaat aaacgcttag agtagtactt atatttgagt gtcctactct
    12481 ctcggatata acctttatag gtactttaga atttataagt aaagtaacat gtgagtgtct
    12541 taaatcatgg aatctaatat gaggtaattc attcttttct aaaaatcttt taaatttttt
    12601 actcataaca tcttcagcaa taggttcaca tttcttattg aaaaat
//
LOCUS       DAYATN010000113        12511 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.113, whole genome shotgun sequence.
ACCESSION   DAYATN010000113 DAYATN010000000
VERSION     DAYATN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.113"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            143..838
                     /gene="cas6"
                     /locus_tag="ACGI8A_002842"
     CDS             143..838
                     /gene="cas6"
                     /locus_tag="ACGI8A_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HFH0180832.1"
                     /translation="MIKCLEMSVKVYLLENISLDNTSKEIRKLIDDTLIIEEKYKEFH
                     NKAGFKNYSFNLLYPLEKDKVYKKDCIYTFIIRTVDSELGMYFMNNLKNQHSDKIKVI
                     TMSKKVIPKKHIESIYSVTPIIAKFEDGYWRSNKTVEDLEKRIRENLIKKYNEFTNSK
                     IDEDFELFQVIKFINRKPISTKYKNISLLGDKISFKVSENDMAQEIANFALGVGVGEI
                     NSRGFGFVNYRWL"
     gene            850..2589
                     /locus_tag="ACGI8A_002843"
     CDS             850..2589
                     /locus_tag="ACGI8A_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HFH0180833.1"
                     /translation="MLKDCLEIFQKEYKSLGNRLITDSYVLSYGTYILVKNFSNIDIL
                     EVSKNNVDSTEDIYHKFCELDYLSKLVSIDKSVDSKKIIHSNNYLSFFVKKENLRPDK
                     NNNVKLTEEIIDNYYDALLNPRIKYKSDKRKKEIYDSIENKYGKVDENKLNKIRLWIK
                     TNIFDLVESVKKDKNYLKIFFDEKIELYNDENQKYLYPNIYNKNDFNVIIDGKNYGLP
                     NDNMQLNEKKPFLKLKTRIRNSTPYLISDDEVLLQKAFFDYLTNKAANGEEVIYISSE
                     KGIHRKTDIPNEFSGYILRIKKGKELEIHDFDTVVNLNTKIKRINVECIIPIEYERHN
                     LKPNLEIGYINNLIKLEKTIDTVFFNKFMCNSYFKEIKNINLSDLVLRDILIRHREGL
                     YTWFYKGRDSIVKQTFSNYSMGIIENSIKNNNYIRAKEQYNLRIAIINYFHGGSNMAD
                     KIKVISESLFEKINSIKTKCIQSDEEYYFAVGQLASYFISKNKSKNKNHSLINPILNA
                     QNRKKIMLEIEKLFKKYNYDISYDNNRRFKNLYSMIVGYEVEEKKVNRDLLIGGYLYY
                     NLIYTKSEENENE"
     gene            2582..3571
                     /locus_tag="ACGI8A_002844"
     CDS             2582..3571
                     /locus_tag="ACGI8A_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HFH0180834.1"
                     /translation="MNRRVYGVLGIKSIMSNWNADFSGYPKTTSTGEVFGSDKALKFP
                     MKKMWENEGEKVLYVKSLKIDESKKGEIKIRPKSLKERYEELFDIEDLKLEKNQQKVL
                     ENLFKAIDVKNFGATFAEEGNNIGIMGAVQIGQGFNKYEDINVEEQQILSPFRDPKAK
                     DKKKSTNEEEKEDANSTTLGKKIVSDEAHYFYPFSINPKAYEVFVNMGVTDGYTKNDY
                     KKFKEAALVSATAFNTNSKLGCENEFALFVETDEELYLPDLAQYITFEKREKSKDLIC
                     INIIHLIAELKDDIKSVEIYYNPYKIEIKPFKDEILPKIDKVKFMNIFTRKEV"
     gene            3573..4313
                     /gene="cas5b"
                     /locus_tag="ACGI8A_002845"
     CDS             3573..4313
                     /gene="cas5b"
                     /locus_tag="ACGI8A_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HFH0180835.1"
                     /translation="MEVLKFNLSGKTAFFKKPDVNSIIYFTYGNIHKIALMGILGACL
                     GLGGYNQQSKEDEYPEFYSLLKDIKMSIVPKHEFGYINKKVHIFNNSVGYASKEEGGN
                     LIIKEQWLENVSWDIYIQLEDSTTSRELKRRFLSNKFVYIPYLGKNDHIANISEIEII
                     TDFYKTEDINRVDSFFIREDFEFEKSYFDDNDDYKEPFKYQEKLPYRLHRMTNQYILE
                     TIIFTNMSVKLINKKEVYQVKGLNLIFI"
     gene            4337..6895
                     /locus_tag="ACGI8A_002846"
     CDS             4337..6895
                     /locus_tag="ACGI8A_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFH0180836.1"
                     /translation="MYFKKVELFDISNNINNCKNIYAHINKDRKPETLKEHSDLSYSY
                     LMKLCVKKDLSSCLERIENKFFKKDSFLSKNFYREIILNMVYMHDIGKINSSFQFVKM
                     QNKNFKKISLRYTKHSFISSMLYFDYYFEKLKKYDFEEKKILSLFLMINTYTISKHHG
                     VLKSFKEFLFLFKDEFINIKENFKDLFKNNYNLELKSVPKTIEKMVRGVEAYLDDSHI
                     KDNLAIDLYIYSKLMFSIVTSCDFYATSEYNSGNSIDDFGTIDNYNKYYDVLKKSEIY
                     KGMKKYREYLNNNSECPYKENDINRLRTEMSIEAEDAITHNTRKRIFYLEAPTGSGKT
                     VTSINLALKSIELNQSLKKIFYIFPFNTLVEQTKDVFINEIFNSVKDIQKDVVVINSI
                     TPIQTEDKEEDNKNVEYCVTKQEIDYDKSLLNRQFLHYPIVLTTNIGFFNYLFGVSRE
                     ECFPLIHMINSVIILDEIQNYKNEIWKEIILFLEKYADILNIKFIIMSATLPRLNKLG
                     CNDDNFAYLVKNREKFFKNHLFKDRVNISFEMLNSEINDIEEISEKVIEEAEIYNKIL
                     IEFIKKSSALKFYKNIKEKLKYKLEDVFLLTGDDNKLERKKIINKIKDMNSVILVATQ
                     VVEAGVDIDMDLGFKDISTIDSDEQFLGRINRSCKKLNSKVYFFNLDDASKIYKKDVR
                     KERHLTLNEESNRKIILDKDFEKYYDKIIARIEENKHKHNDKNIEVFIKDIVGNLNFT
                     EVKKWMALIPDLKEYTIFLNTIVKDESGNMIVGSDVWYEYISLLKNDNVEYSEKRVKM
                     SEIMEKLDYFTYKVQKFDNSFNDLVGDIFYIDDGSKYFTEGKFDRSKFNQNEFL"
     repeat_region   7085..10086
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=7085..7114
                     /rpt_unit_seq="gttaaacagtaacatgagatgtatttaaat"
     gene            10171..10665
                     /locus_tag="ACGI8A_002847"
     CDS             10171..10665
                     /locus_tag="ACGI8A_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HFH0180837.1"
                     /translation="MKITGTLINYYFHCKRQCWLLGNRINLEENSEDVKIGRLLHELK
                     EEKTKYKEIAIENIKIDKLTREYLVEVKKSDADIEAVKWQVLLYLKKLKEKGIVRKGK
                     IEFIEKNKTNKKIVFVDLSEVSEEQLISIEREIVNLINSDKAPCPDLKKGCKKCAYYE
                     YCYI"
     gene            10691..11698
                     /gene="cas1b"
                     /locus_tag="ACGI8A_002848"
     CDS             10691..11698
                     /gene="cas1b"
                     /locus_tag="ACGI8A_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HFH0180838.1"
                     /translation="MLILGKTKYITSMGELTRKDNSICFRKNGRNVYIPVENTSEIFC
                     LNEVSLNSKLLDFLSRANIVVHFFNYYGGYSGTFYPKEYLISGKVTVAQVKTFEDRRE
                     EIAKSIVNGIRINAINTLSHYYKHGNSEIQPLLSHLKKDSINKLEKSNNIKEILSVEG
                     DIWQTFYTSFKYILREEFIFNKRVKRPPDNPLNAMISFGNSILYAKTVTAIYNTHLNQ
                     SVSFLHEPSESRFSLSLDLSENFKHVITFKTIFDLVNNRKINIEKHFDKNLNYCVLND
                     EGKQIFINSLEERLESVKEHPKLKRKVSLKTCIKLDAYKMLKCIMEEKSFIPYNIKDG
                     L"
     gene            11909..12199
                     /gene="cas2"
                     /locus_tag="ACGI8A_002849"
     CDS             11909..12199
                     /gene="cas2"
                     /locus_tag="ACGI8A_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFH0180839.1"
                     /translation="MKKKNNLNYNYVFVVYDVGEKRVSKVFKVCKKYLSHFQKSVFRG
                     EITPSKIMSLKRDLENIIDKDNDFVCIIKLMNSNTFEEEILGNNSNCENMIL"
BASE COUNT         5375 a         1099 c         1897 g         4140 t
ORIGIN      
        1 tcgaataaaa aataaaaaat tttaaaggaa aaagttaaaa aataaagaat tttataacta
       61 taaattatat tttagagttt attgttatta aatagatgaa aaaatcctaa tatggattat
      121 cagaaaatga ggaggttgtt aattgataaa atgtcttgaa atgtctgtaa aagtgtatct
      181 tcttgaaaat atttctttag ataatacatc aaaagaaata cgtaaattaa tagatgatac
      241 attaattata gaagaaaaat ataaggaatt tcataataaa gctggattta agaattattc
      301 ttttaattta ttatacccat tagaaaaaga taaggtttat aagaaggatt gtatatatac
      361 ttttattatt agaactgtag atagtgaatt aggaatgtat tttatgaata atttaaaaaa
      421 tcagcattct gataaaataa aggttattac aatgagtaaa aaagtgatcc ctaaaaaaca
      481 tatagaaagt atttactctg tcactccaat aatagctaaa tttgaggatg ggtattggag
      541 aagtaataaa acagtagaag atttagaaaa aagaatcaga gaaaatttaa taaaaaaata
      601 taatgaattt actaacagta aaatagatga agattttgaa ttatttcaag ttattaaatt
      661 tataaatagg aaacctattt ctactaaata taagaatata tctttacttg gtgataaaat
      721 aagttttaaa gtatcagaaa atgatatggc tcaggaaatt gcaaattttg cacttggagt
      781 tggagtcgga gaaattaatt ctcgtggatt tggttttgta aactatagat ggttatagga
      841 ggtgaaaact tgttaaagga ttgtttagag atttttcaaa aagaatacaa atctttagga
      901 aatagattga taactgatag ctatgtccta tcctatggaa catatatatt ggtgaaaaat
      961 ttttcgaata tagatatatt agaagtttct aaaaataatg tggatagtac agaagatata
     1021 taccataaat tttgtgaact ggattattta tcaaaactgg tgtctataga taaatcagtt
     1081 gattccaaaa aaataataca tagtaataat tatctttcat tttttgtaaa aaaggaaaac
     1141 ttaagacctg ataaaaataa taatgtaaaa cttacagaag aaattattga taattattat
     1201 gatgctttgt taaacccaag aattaaatat aaatctgata aaagaaaaaa agaaatctat
     1261 gattcaatag aaaataaata tggtaaagtg gatgaaaata aattaaataa aataagacta
     1321 tggataaaaa caaatatatt tgatttagtt gaatctgtta aaaaagataa aaattattta
     1381 aaaatatttt ttgatgaaaa aatagagtta tacaatgatg aaaaccaaaa atatttatat
     1441 ccaaatatat ataataaaaa tgatttcaat gtaattattg atggtaaaaa ctatgggtta
     1501 ccaaacgata atatgcaatt aaatgaaaaa aagccatttt tgaaattaaa aacaagaatt
     1561 agaaattcaa ctccttattt gattagtgat gatgaagttt tgttacagaa agcttttttt
     1621 gattatctaa cgaataaagc agcgaatgga gaagaagtaa tatatataag tagcgaaaag
     1681 ggaattcata gaaaaactga tataccaaat gaatttagtg gttatatact gagaataaaa
     1741 aagggtaaag aattagaaat acatgatttt gatactgtgg taaatttaaa cactaaaata
     1801 aaaagaataa atgtagaatg tataatacca atagaatatg aaagacataa tttaaaacca
     1861 aatttagaga taggatatat taataatctt ataaaattgg aaaaaacgat tgatactgta
     1921 ttctttaata aatttatgtg caatagttat tttaaagaaa ttaaaaatat aaacttaagt
     1981 gatttagttc taagggatat acttattaga catagagaag gtttatatac ctggttttat
     2041 aaagggagag atagtatagt aaagcaaact ttttcaaact attctatggg tataattgaa
     2101 aattctataa aaaacaataa ctacataaga gcaaaggagc aatataattt aagaatagct
     2161 ataataaatt attttcatgg aggaagtaat atggcagata aaataaaagt tattagtgaa
     2221 tctttatttg aaaaaataaa ttctataaaa acgaaatgta tacaaagtga tgaagaatat
     2281 tattttgcag ttgggcaatt agcatcgtat tttatatcta aaaataagtc aaaaaataaa
     2341 aatcattctc tgattaatcc tatactaaat gcacaaaata gaaaaaaaat catgttagag
     2401 atagaaaaat tatttaaaaa atataattat gatataagtt atgacaacaa taggcgattt
     2461 aagaatctat attctatgat tgtaggatat gaggtagaag aaaaaaaagt taatagagat
     2521 ttattaattg gtggatatct atattacaat ttaatataca caaaaagtga ggagaatgaa
     2581 aatgaataga agagtatatg gagtattagg tataaaatct ataatgtcaa actggaatgc
     2641 tgatttttct ggatatccaa agacgacatc aacaggagaa gtttttggga gtgataaagc
     2701 acttaagttt cccatgaaaa aaatgtggga aaatgaaggt gaaaaagttt tatatgttaa
     2761 atcattaaaa atagatgaat ctaagaaagg agaaataaaa attagaccaa aatcattaaa
     2821 agagagatat gaagaattgt ttgatataga ggacctaaaa ctagaaaaga atcaacaaaa
     2881 agtactggaa aatctattta aagctattga tgttaaaaat tttggtgcaa catttgcaga
     2941 agaaggaaat aatataggaa ttatgggcgc agtacaaatt ggacaaggat ttaataagta
     3001 tgaagatatt aatgttgaag aacaacaaat attatcccca tttagggatc caaaagcaaa
     3061 agataagaaa aagtcaacaa atgaagaaga aaaagaagat gcaaattcaa ctacattagg
     3121 aaaaaaaata gtaagtgatg aggctcatta cttttatcca ttttcaataa atccaaaagc
     3181 ttatgaagtt tttgttaata tgggtgtgac tgatggatat actaaaaatg attataaaaa
     3241 atttaaagaa gctgctttag taagtgcaac tgcattcaat acaaattcaa agcttggttg
     3301 cgaaaatgaa tttgcattat ttgttgaaac tgatgaagag ttatacctac cagatttggc
     3361 acaatatata acttttgaaa agagagaaaa atcaaaagat ttaatttgta taaatataat
     3421 tcatcttata gcagaattaa aagatgatat aaagagcgtt gagatatatt ataacccata
     3481 taaaatagaa ataaagcctt ttaaagatga aatattacct aagatagata aagttaaatt
     3541 tatgaacata tttacaagaa aagaggtata atatggaggt tttgaaattt aacttaagtg
     3601 gaaaaactgc tttttttaaa aaaccagatg taaatagcat aatttatttt acgtatggaa
     3661 acatacataa aattgcactt atgggtattt taggagcttg tttaggactt ggtggatata
     3721 atcaacagag taaggaagat gagtatccag aattctactc tttacttaag gatataaaaa
     3781 tgtcaatagt tccaaagcat gaatttggat atataaataa aaaagtccat atatttaata
     3841 attctgtagg atatgcatca aaagaagaag gtggaaattt aataataaaa gaacagtggc
     3901 ttgaaaatgt tagttgggat atatatatac aattagaaga ttcaactaca agtagagagc
     3961 ttaaaagaag atttttatct aataaatttg tatatatacc ttacttaggc aaaaacgatc
     4021 atatagctaa tataagtgaa atagagataa ttactgattt ttacaaaaca gaagatatta
     4081 acagagtgga cagtttcttt ataagagagg attttgagtt tgaaaaatct tattttgatg
     4141 ataatgatga ttataaagag ccatttaaat atcaggaaaa acttccttat agactgcacc
     4201 gaatgactaa tcaatatatt ttggaaacta tcatatttac taatatgtca gttaaattga
     4261 ttaataaaaa agaggtatat caagtaaagg gtttaaattt aatctttatt taaatttata
     4321 ggtttaggag aattatatgt actttaaaaa agttgagttg tttgatataa gtaataatat
     4381 aaataattgc aaaaatattt atgctcatat aaataaagac aggaagccag aaactttaaa
     4441 agaacattca gacttatcat actcatatct tatgaaatta tgtgtaaaaa aagatttaag
     4501 ctcttgccta gagagaatag agaataaatt ttttaaaaaa gattcttttt taagtaagaa
     4561 tttttataga gagattatct tgaatatggt ttatatgcat gatataggta agattaattc
     4621 ttcatttcag tttgtaaaaa tgcaaaataa aaattttaaa aaaatttcac ttagatatac
     4681 taaacattct tttatctctt caatgctata ttttgattac tattttgaaa agcttaaaaa
     4741 atatgatttt gaagaaaaaa aaatattgag tttatttttg atgataaata catatacaat
     4801 atcaaaacat catggggttc tgaagtcatt taaagaattt ttatttttat ttaaagatga
     4861 gtttataaac attaaagaaa attttaaaga tttatttaaa aacaattata atttagagct
     4921 taaatcagtt cccaaaacta tagaaaaaat ggtaagaggt gtagaagcgt atttagatga
     4981 tagccatata aaggataact tagctataga cttatatata tattcaaaat tgatgttttc
     5041 aattgttaca tcttgtgatt tttatgctac atcagaatat aatagtggta attctataga
     5101 tgattttggg acaattgata attataataa gtattatgat gtattaaaaa aatctgaaat
     5161 atataagggg atgaaaaaat atagggaata cttaaataac aatagtgaat gcccatataa
     5221 agagaatgat ataaacagat tgagaacaga aatgagtata gaagctgaag atgcaattac
     5281 acataatacg agaaaaagaa tattttattt agaagcacca actggaagtg gaaaaactgt
     5341 tacatctata aatctagcac tgaaatctat agagttaaat caatctttaa aaaagatatt
     5401 ttatatattt ccatttaaca cattagtaga gcaaacaaaa gatgtattta taaatgaaat
     5461 atttaattct gttaaagaca tacaaaaaga tgttgtagtt ataaattcaa taacaccaat
     5521 acaaacagaa gataaagaag aagataataa aaatgttgaa tattgtgtta ctaaacaaga
     5581 aattgattat gataaatcat tgttaaatag acaattttta cactatccta tagtacttac
     5641 aactaatata ggttttttta actatttgtt tggtgtatca agagaagaat gttttccatt
     5701 gatacatatg ataaatagtg taattatatt agatgaaata caaaattata aaaatgagat
     5761 atggaaagaa attattttgt ttttagaaaa atatgcagat atattaaata taaaatttat
     5821 tataatgtct gcaactttac ctagattaaa taaattagga tgtaatgatg ataattttgc
     5881 atatctagta aaaaacagag aaaaattttt taaaaatcac ctttttaaag atagagtaaa
     5941 cataagtttt gagatgttaa attcagaaat taatgatatt gaagaaatat cagaaaaggt
     6001 tatagaagaa gcagaaatat ataataagat actgatagag tttataaaga aatcatcagc
     6061 actaaaattt tataaaaata ttaaagaaaa attgaagtat aagttagaag atgttttttt
     6121 attaacaggt gatgataata aattagaaag aaaaaaaata ataaataaaa ttaaagatat
     6181 gaactctgta attttggttg ctacacaagt tgtagaggct ggagtagata tagatatgga
     6241 tttgggattt aaagatatat ctacaattga ttctgatgaa caatttttgg gaagaataaa
     6301 tagatcttgt aaaaaattaa attcaaaagt atactttttt aatttagatg atgcaagtaa
     6361 gatatataag aaagatgtta gaaaagagag acatttaact cttaatgagg aaagtaatag
     6421 aaaaattatt ttagataaag actttgaaaa gtattatgat aaaataatag ccagaataga
     6481 agaaaataag cataaacata atgacaagaa tatagaggtt tttataaaag atattgtggg
     6541 aaatttgaat tttacagagg taaaaaaatg gatggcttta ataccagatt taaaagagta
     6601 tacaatcttt ttaaatacta tagttaaaga tgaatctgga aatatgattg taggaagtga
     6661 tgtatggtat gaatatatat cactactaaa aaatgataat gttgagtatt ctgaaaagag
     6721 ggtgaaaatg tctgaaatta tggaaaaact agattatttt acgtataaag ttcagaaatt
     6781 tgataattca tttaatgatt tagttggaga tattttttat attgatgatg gaagtaagta
     6841 ttttacagag ggaaagtttg atagaagtaa atttaatcaa aatgaatttc tataatgtat
     6901 ttagattata tagagaatat ttatcccaag ttaattaatt agaattagtt taaaactatt
     6961 gaaatataga tattttatag cttatatatt ttttctagga ttagctggga taaaaaatct
     7021 gtatattagt gttttcagtg tatttaaggg gattatattt ttaagaagta ctgattttaa
     7081 agtggttaaa cagtaacatg agatgtattt aaatttttac tacataatta aatcgtagtc
     7141 aataccgtac aggttaaaca gtaacatgag atgtatttaa atctctgaat agccaatcaa
     7201 ctttgctgca ttactcttgt taaacagtaa catgagatgt atttaaatga taatagttat
     7261 atatattttt tacaatatat tattgttaaa cagtaacatg agatgtattt aaatttactt
     7321 ctcctgtttt gaaatctata tattcatcat gttaaacagt aacatgagat gtatttaaat
     7381 ataatattga tacaaatatt ttgggtaaat ggacttgtta aacagtaaca tgagatgtat
     7441 ttaaattcga tatggtaaca aatttataaa agatatagaa tgggttaaac agtaacatga
     7501 gatgtattta aataaagaaa aacgaaattg gtgattttga agcaatgtgt taaacagtaa
     7561 catgagatgt atttaaatta gtaactacaa tgcttttcct tttgaagatg atatgttaaa
     7621 cagtaacatg agatgtattt aaattaataa tgcacctatt ttaacagtac ctggtctaaa
     7681 gttaaacagt aacatgagat gtatttaaat tcaagtctag gagacaacat aaatagtaca
     7741 gatgggttaa acagtaacat gagatgtatt taaattagta aaaccagatg gaactgttgt
     7801 aataccacca aagttaaaca gtaacatgag atgtatttaa atttaaaaac atcttgagga
     7861 atatctttaa cagtaattgt taaacagtaa catgagatgt atttaaatgc attttcaggt
     7921 gcatgataaa taggtaaatt agatgttaaa cagtaacatg agatgtattt aaattttttg
     7981 taattagcca gattatcaac tcccaaaata gttaaacagt aacatgagat gtatttaaat
     8041 taaatatcgt ccttcgtagt caaagcaaca ccagaaggtt aaacagtaac atgagatgta
     8101 tttaaatcgt aaacaaaatt tatcaagaga agaatttcta aggttaaaca gtaacatgag
     8161 atgtatttaa atttatattt gataagagta tcaaatcgac ttaaaatagt taaacagtaa
     8221 catgagatgt atttaaattc aatcttaata cacagacatt tttaaaaaca aaacgttaaa
     8281 cagtaacatg agatgtattt aaataaatag acaaatattg aatagctctg tcttcatcaa
     8341 gttaaacagt aacatgagat gtatttaaat atatttacaa aatctctaac atatttttta
     8401 acttctgtta aacagtaaca tgagatgtat ttaaattctt ttaaagagtc ctcgggagcc
     8461 tcgaaaactt cagttaaaca gtaacatgag atgtatttaa attactcaaa taaatttcta
     8521 atttgtttat ctactaacgt taaacagtaa catgagatgt atttaaatta gaatttacaa
     8581 caatataagc ctctttaacc ataagttaaa cagtaacatg agatgtattt aaatatgttt
     8641 ttagtagtac atttaataaa actttttaaa gttaaacagt aacatgagat gtatttaaat
     8701 gaaggaaccg taccttgata aatcacgacc gtacccgtta aacagtaaca tgagatgtat
     8761 ttaaattgta tatttgatgt tgttatttct cctgtgtata atgttaaaca gtaacatgag
     8821 atgtatttaa atgaagttcc acccccacca cccccactac tttgagaagt taaacagtaa
     8881 catgagatgt atttaaatta acaatagagt aaatataata aacaagcata caaagttaaa
     8941 cagtaacatg agatgtattt aaatcccctt ttgcatggta ctagacacgc atatatggtg
     9001 gttaaacagt aacatgagat gtatttaaat acgcctatat gcggagtaac agatttttgc
     9061 ctggatggtt aaacagtaac atgagatgta tttaaatact gttttgggat agactggaat
     9121 taaatggtct gtgttaaaca gtaacatgag atgtatttaa atttttggaa tgaagcatca
     9181 acagataata aagaaaaagt taaacagtaa catgagatgt atttaaatca aggaaacaaa
     9241 agagaagtga aaagaaatat aatgttaaac agtaacatga gatgtattta aataatcttt
     9301 taaatggaaa ttaagtaata aaagagaggg ttaaacagta acatgagatg tatttaaatt
     9361 caattaaata agcattaatc ttagtaaaat caaacgttaa acagtaacat gagatgtatt
     9421 taaattttct tgtgttaatt gaacccccat tttaagctcc agttaaacag taacatgaga
     9481 tgtatttaaa tcagaataca gcttctatat ctggatacat ttgtcttggt taaacagtaa
     9541 catgagatgt atttaaatgt gaaaagtaat acaggtatag agtctaaacg ctcggttaaa
     9601 cagtaacatg agatgtattt aaatcaatgt atcctctttt ttagttttgg agcctctttt
     9661 gttaaacagt aacatgagat gtatttaaat tagttagcac attttatttc aagccttaaa
     9721 acattcgtta aacagtaaca tgagatgtat ttaaattaat ttgcacactt aacctctaat
     9781 cttaaagtat tagttaaaca gtaacatgag atgtatttaa ataacacaat attatcttta
     9841 atagaattaa acaaatgagt taaacagtaa catgagatgt atttaaattt ttgttggtct
     9901 tgtgcttctt ttaaaagctg attagttaaa cagtaacatg agatgtattt aaatttaaaa
     9961 agttctgtta ttaaaacatc caaaccaatc gttaaacagt aacatgagat gtatttaaat
    10021 tctgtaccat ctagccaaac cgtttttgaa gttaaagtta aacagtaaca taagatgtat
    10081 ctaaattact ctatcataaa attttaatat attaaacaaa ctaatatgtg atatacttat
    10141 gtataaataa caaaataaat aggtgatgtt atgaaaatta caggaacttt gataaattac
    10201 tatttccatt gtaaaagaca atgctggttg cttggtaata gaataaattt ggaagaaaat
    10261 agtgaagatg taaaaatagg tagacttctt catgaactaa aagaagaaaa aacaaaatat
    10321 aaagaaattg caattgaaaa tataaaaata gacaaattaa caagagaata tttagtagag
    10381 gttaaaaagt ctgatgctga tatagaagct gttaagtggc aagttctttt atatttaaaa
    10441 aagttaaaag aaaagggaat tgtaagaaaa gggaaaattg aatttataga aaaaaataaa
    10501 actaataaaa aaatagtctt tgtagatttg agtgaagtat ctgaagaaca gcttatatct
    10561 atagaaagag aaattgttaa tttaataaat agtgataaag caccatgtcc tgatttaaaa
    10621 aaaggatgta aaaaatgtgc ctattatgaa tattgttata tataaagagg tataagaaac
    10681 atataaggag gtgttaatat tgggaaagac aaaatatatt acctcaatgg gtgaactcac
    10741 aagaaaagat aattctatat gttttagaaa aaatggaagg aatgtatata taccagtaga
    10801 aaatactagt gaaatattct gtctaaatga ggtatcttta aattcaaaat tactggattt
    10861 tttatcaaga gcaaatattg ttgttcattt ttttaattac tatggtggat atagtggaac
    10921 tttttatcct aaagaatatt taataagtgg aaaagtaaca gtggcacagg taaaaacctt
    10981 tgaagataga agagaagaaa tagcaaaatc catagtcaat ggtataagga ttaatgcaat
    11041 aaatacatta tcgcattact ataagcatgg aaattcagaa atacaaccat tattatcaca
    11101 tctaaaaaaa gattctataa acaagttaga aaaatcaaat aatataaaag aaatattatc
    11161 agtcgaagga gatatttggc aaacatttta tacttccttt aagtatattt taagagagga
    11221 atttattttt aataaaagag ttaagagacc accagataat cccttaaacg caatgatatc
    11281 atttggtaac agtattttat atgctaaaac tgtaactgct atatataata cacatctaaa
    11341 tcaaagtgta agttttttac atgaaccaag tgaaagtaga ttttctttaa gtctagattt
    11401 aagtgaaaat tttaaacatg ttataacttt taaaactata tttgatttag ttaataatag
    11461 aaaaataaat attgaaaaac attttgataa aaatttgaac tactgtgttc taaatgatga
    11521 aggaaagcaa atttttataa attctttaga agaaagatta gaatcagtca aagaacatcc
    11581 aaaattaaaa cgaaaagtaa gtttaaaaac ctgtataaaa ttagatgctt acaaaatgtt
    11641 aaagtgtata atggaagaaa aaagttttat tccatataac ataaaagacg gattataatt
    11701 taaacataaa gtaagtattt taatatagaa ctttgatata tataagaatg ataattatgc
    11761 tattataagt atacataata tattattgaa ataatataag tttttagaag tattttaaga
    11821 tgagaaagga gaaaatactg aaaaataatt attctattat aatttgccaa ggttttgtat
    11881 atttttcagt ttaaatatta tattacatat gaaaaagaaa aacaatttaa attataatta
    11941 tgtatttgta gtctatgatg taggagaaaa aagagtaagt aaagttttta aagtgtgtaa
    12001 aaaatatctc tcgcattttc aaaaatcagt ttttagaggt gaaataactc catctaagat
    12061 tatgagctta aaacgtgatt tagaaaatat tatagataaa gataatgatt ttgtatgtat
    12121 aataaagttg atgaattcta atacatttga agaagaaata ttagggaata atagtaattg
    12181 tgaaaatatg atattataaa tctaattact atatttgtaa aaaataatag cttattaaag
    12241 agtagtaaaa ttgggatata atttatgata gagactgcaa gtagattttt gtgaaaagga
    12301 tttacttgca gtatttttgt tatgtataac tgttaacatt agtgtacaaa acattatcaa
    12361 tagcattttt ctataatgaa gaaatactat tataatggga aaattttatt aatactaggt
    12421 aataatatat ttacacaagt ctatatatat tataattttc attataatta ttcagtattt
    12481 ctaataataa gattcttttt gtatttattt a
//
LOCUS       DAYATN010000114        12505 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.114, whole genome shotgun sequence.
ACCESSION   DAYATN010000114 DAYATN010000000
VERSION     DAYATN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.114"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            414..776
                     /locus_tag="ACGI8A_002850"
     CDS             414..776
                     /locus_tag="ACGI8A_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0180840.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            874..1458
                     /locus_tag="ACGI8A_002851"
     CDS             874..1458
                     /locus_tag="ACGI8A_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HFH0180841.1"
                     /translation="MDALREIGNIGSGNAITALASMINSNVEVLIPMVKILEYNEATN
                     LLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAFLDEIEAIEISA
                     IKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRFSDVGDKVLFIE
                     NKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            1455..1940
                     /locus_tag="ACGI8A_002852"
     CDS             1455..1940
                     /locus_tag="ACGI8A_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HFH0180842.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            1983..2501
                     /locus_tag="ACGI8A_002853"
     CDS             1983..2501
                     /locus_tag="ACGI8A_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFH0180843.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            2637..3596
                     /locus_tag="ACGI8A_002854"
     CDS             2637..3596
                     /locus_tag="ACGI8A_002854"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0180844.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            3622..5325
                     /locus_tag="ACGI8A_002855"
     CDS             3622..5325
                     /locus_tag="ACGI8A_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFH0180845.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            5632..7734
                     /locus_tag="ACGI8A_002856"
     CDS             5632..7734
                     /locus_tag="ACGI8A_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFH0180846.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            7747..8187
                     /locus_tag="ACGI8A_002857"
     CDS             7747..8187
                     /locus_tag="ACGI8A_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFH0180847.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            8223..9026
                     /locus_tag="ACGI8A_002858"
     CDS             8223..9026
                     /locus_tag="ACGI8A_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFH0180848.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDRTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            9066..9662
                     /locus_tag="ACGI8A_002859"
     CDS             9066..9662
                     /locus_tag="ACGI8A_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HFH0180849.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            9768..10631
                     /locus_tag="ACGI8A_002860"
     CDS             9768..10631
                     /locus_tag="ACGI8A_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HFH0180850.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            10698..11687
                     /locus_tag="ACGI8A_002861"
     CDS             10698..11687
                     /locus_tag="ACGI8A_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFH0180851.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            11779..12390
                     /locus_tag="ACGI8A_002862"
     CDS             11779..12390
                     /locus_tag="ACGI8A_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0180852.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         5059 a         1222 c         2347 g         3877 t
ORIGIN      
        1 acatatagag aaatataatt aagtaaataa acaactatct attagatatt gagaaagtca
       61 aatcctatat gaatctacag aaatgatttt aatgaatctg aaaatttttt ttatacaaac
      121 ataaaaaagg tataaagttg ttttattata atttaattac ttttgataac tttttatgtt
      181 ttatacaatt atatttttaa taactcgtca aaacgccaaa catatattac aatagatata
      241 taagtttttt tgttgacaaa ttatgttaag agaattaagt ataatatggc ataaataatt
      301 acttttattt atcaatgaaa tgtatttatt gtgtagcgaa aaatagtcag atagtataga
      361 caataactga agataaaagc gtttattgtt gtacatatgg aggagttagt tttatggaaa
      421 ataaaatttt gatagtagat gatgctattt ttatgagaat gataattaaa gaagctctga
      481 gtaaaagtgg ctataataat ttaatagaag caaatgatgg agaagaagcg tgtaatatat
      541 ttaaatatga aaagcctgat ttgatgttgc ttgatattac aatgccaaaa aaagatggtt
      601 tagaggtgct aagagaaata aaaaagttgg attcaagtgc taaggttgta atgtgctctg
      661 caataggaca agagaatatg attgtagaag ccataaaact tggagcgtta gattttatag
      721 tgaagccctt caaaacagaa acattagtta aagttgtaga taatgcttta aaataaaagt
      781 gtaaatatac actaatacaa tgaaaagcaa ataaatgcaa taataaaggg aggggtttat
      841 attataaatt atttagaact tgatgatgtt catatagatg ctcttagaga aattggtaat
      901 ataggctctg ggaatgcaat tactgcactt gcatccatga taaatagcaa tgtagaagta
      961 ttaataccta tggtcaagat acttgaatat aatgaagcga caaatttgct tggtggtcca
     1021 gaaaacaaag tagtatgtat tttactcgat atgaaaggtg atatcaatgg aatgtttatg
     1081 tttttgttgg atgaatcaat aactcaactt atgctaagtt ctctttttaa taaagaagaa
     1141 gcttttttag atgagattga ggcaattgaa atttcagcaa ttaaggaaat aggaaatatt
     1201 atggcaagtt catatgtaaa tgctattgca tctatgttaa acatgactat aagtgtttca
     1261 attccagata tatgtattga tatggttgga gctgttctaa atgtacctat gataagattt
     1321 tcagatgttg gtgacaaagt cttatttata gaaaataaat ttaaaatgag tgataattat
     1381 tttactagtc acattttgat gattccagaa atgagctcac ttagagaaat actcgtgagg
     1441 ttgggattag aagtatgaat agagagatag tagtgaacat tgcagatatg aagatagcct
     1501 atagaccaaa tgttttagtt acttatgcac ttggttcttg tgtaggagta tgtttgatag
     1561 ataaagtagc tggaattggt ggaatgttac acgttatgtt gccatatagt aaagatgcta
     1621 ttaacattga aaataagtgt aaatttgctg acacaggaat aaatgagctc attaaatcta
     1681 tggagaatat aggtgcaaat agaatatata taaaagcaaa aattgctgga ggtgctcaga
     1741 tgtttttagg aagtagcaat tcagtaattg caagtatagg acataggaat gttgaggcag
     1801 taaaaaaaac tcttttagga ttaaatattc caatattagc tgaggacaca ggtatgaatt
     1861 atggtagaac aatacgtttt tacactgaga cgggtgaatt acttgtagat tcaataaata
     1921 aaggtaagaa gaaattataa tatatatttc atagaaagtg tattttagga gggaggtatt
     1981 aaatgagatt tccagatgac ccatatttag attatggact aattgatgaa ggaattaagt
     2041 atctaaagtt taaagtagat gagcaagatt ttggggttga acttgaaaag gtaatagaaa
     2101 ttactggaaa tcaagaaatt atatttattc cagaactacc tacatattct aaaggcataa
     2161 taaatctaag aggaaaaatc attccgataa tagatatgcg tttgagactt aaaaaagatg
     2221 aattaatgac tacaaattgc atgtatatag tagtaactga aataaaggac ttggtagttg
     2281 gatttgtggt tgatagagtt gatgatatag taagcttaga aaaaagtaaa atatcacctc
     2341 caccaagagt tactatagaa tattcggatt attttgtaag tggagttgga gaattagaag
     2401 gtaaaataat tactttacta gatttggaga aacttttgac agataaagat gaagttttaa
     2461 tagatgaatt aatcaatggg tttgattgca ctatcatgta acatagggat gtagttggag
     2521 tgttattata aaaagattgt gatgagaaca tttaagaata tagtggtata attattaagt
     2581 ataaaaattt taaatcatta aaatttgaat atattatgtt taagagaggt ttgtatatga
     2641 aaaataagca acagggaaca attttagtcg ttgatgatag tgttctgatg tgtgacttaa
     2701 tagataaaac acttacaaaa gataatttta aggttaaaaa agcatttaat gcaatagagg
     2761 caaaagaatt tattgaagaa tttataccag acattatttt gcttgatata attttaccag
     2821 ataactctgg ttttgagttt tgtaaggaag taaaatctaa tgctcgtaca gcagatatac
     2881 ctataatttt tataacttca aaaaatacag atacagacat agtaaaagga tttggtgtag
     2941 gagcaattga ctatatggtg aaacctttta gtatgacaga attaaaagct cgtgttatag
     3001 ctcatcttga agtaaagaaa tctcaagata aattaaaaaa gataaataat gaactggaat
     3061 cttctcttga aaaattgaat agactcgttg taagagatta tcttacaggt ctttacaata
     3121 ggcgacatat aattaatcag cttatggagc aaagaagaat taataaatat agtgaaacat
     3181 caatatccct tatccttggt gatattgatg actttaaggt tatcaatgat acttatgggc
     3241 atgaagcagg ggatttcgtg ttaacagtaa tatcaagtat aataaaaaag aattgtagac
     3301 aaattgatac tgtatcaaga tggggtggag aagagttttt gataatgctc acaaatactc
     3361 caatagaagg tgctaagata ctttcagaaa gaatccgtaa agagataaag gagtttgatt
     3421 tcaactataa agaacataaa ataaagtgca ctattacatt aggagtagta gaagtaaact
     3481 caaatatatc attagaagaa aacatatcct tagcagataa agcaatgtat gagggtaaaa
     3541 acttaggaaa agattgtagc gtagtgagta caatgaaagg attaaaaaag atataaactt
     3601 actttatgaa ggagtacaga tatgaaaaga gaagtaaagc tttctaaatt agcaattgta
     3661 ttgctgatta ttatagtatt ggctgttcta gcagggattt tttcttctat ttcatcttat
     3721 aaagcatttg aaaaagtaga aaaaagtatt aatgatatga ataatttgag gactcaaagt
     3781 cagtatataa aagatacaac tagagagata agtgatgtat taagaagata tgtatttaca
     3841 ggagaaaaaa agaataaaga tatttatgaa aaagaattat acggaaaaac aagagaatca
     3901 gctttaaaca aattagaaaa aattggtctt acaaagagcg agtcaaataa aatcttagaa
     3961 gcaaaagaaa tatcagattc tttagtacca acagaacaaa gtgcaataaa ggcagtcgaa
     4021 agtaatgata agagtaaagc tcaaagtata atttttggta gtgactatat aaactcccaa
     4081 gagaaaatgg attctttaat aaatgaattt caagaggata tagttacccg ttcaaattcc
     4141 aaaattgtag aagtaaaaaa agaattacaa agtctaatat taagaacaat atttatactt
     4201 tcccttttaa taatattgac aataatagaa tatacagtaa taaaattaaa aatagtgatt
     4261 cctattaata aagtagaaaa acacttttca agaattgcat ctggggactt aagaacaagt
     4321 atagacgttg aagaaagtaa atctgaaata gggctattag tgagttctgt caaaaagatg
     4381 caaagtatgc ttttaaatta catagacctt atagacagta cattaactag tattgcaaaa
     4441 ggagatttaa gagtagagat agaccaagaa tttataggtg attttaaatc aattaaaata
     4501 tctttagaaa gtataataaa ctcttttaat ggtgattttt ctgaaataaa tttatcagca
     4561 gagcaagttg caagtgcttc agaacaagtt gcagcaggtg cacaagctct ttcacagggt
     4621 gctactgaac aagctagttc agtagaagaa ttagcttcca cgattaatga gatatcagat
     4681 ggaattaaga agtgtgcaga tagtgctagt cttgcatgtg aggtatctga ccaatctgca
     4741 tcacaagttg gttttggaag tagatgtatg gatgatatga tgacatcaat gggtgaaata
     4801 agtgaaaaat cgagtgaaat atcaaaaata atcaaaacaa tagatgagat agcattccaa
     4861 actaatattc ttgcattaaa tgcagctgtt gaagcagcaa gagcaggtca atatggtaga
     4921 ggatttgcag ttgtagcaga tgaagttagg tctttggctg gtaaaagtgc aaaagcagct
     4981 caaaatacag ctattctcat agaagaagct attaaaacag tagaaaatgg tgtcagaatt
     5041 gctaaagaga ctgcaaatgc attggacttg gtagtaaatg gtgtagaaaa aactactaat
     5101 tatattaatg gtatttcttc ggctgtacat agtcaagaag tagcaattac acagatttta
     5161 tctggaatag agcaaatttc catagtagtc caaatgaact cagcaacagc agaggaaagt
     5221 gcagcagcct cagaagagat gtctgctcag gctcaaattt taaaagaatt agtagaaaaa
     5281 tttcatctaa aggaaagtga agtaagagaa tcatcatggg tttaaattat gaggtgaata
     5341 ttgaagtaag agaattatca tgtatttaaa ttatgagatg tatatatatt gaaataagag
     5401 aattatcaag gatttaaatt atgagatgta tatatattga agtaagagaa taattgtgta
     5461 tttaaattat gaggtacata tagtgagata agagaataat tgtgtattta aattatgagg
     5521 tacatatagt gagataagag aatcatcaag gatttaaatt atgagatgca tatataatag
     5581 aaaattattt gtaggtcgta ggtgataaat gtgaattata ttgaaccgag tatgagacaa
     5641 atgttagata tgtttatatt tgagacaagt ttattactcg aacaattaga tgaaattatg
     5701 attcaaatag aaaaagagaa agtatttact cataacaaca ttaatgaaac ttttagaata
     5761 atgcatacga taaaaggttc agcatctatg atggggttag acaatatatc tgctttagca
     5821 catagtgtag aagacttgtt ttatgctata cgtgaaggta aaatagaagt tttagacaat
     5881 gacctattat ttgatttagt ctttcaatca ttagatttta taagaagaga tatagatgag
     5941 ttacaatcaa gaggttgtat atcttctgat tactctaatt ttattgaagg aattgaagag
     6001 tatgtgagta ctttggaatt atcaagttct atgaaatcaa aggataatgc aaatttagaa
     6061 agtaaagacg aaaaagaagt aatcgattca aattttgaat acaaagaaga ctctgatgta
     6121 gtaaaaataa tctttgaaca aggttgcatg atggaaaatg tgagagcttt catgcttgta
     6181 tttgaactta gagattactg tgaatttata gatttttatc caaaagatgt agaaacaaat
     6241 gcagagtcta cagagtatat taaaagttat gggttttata tacaatttgt agctaaagag
     6301 gataaggcaa tagtttataa aactatagaa gataatctct atgtaaaaga atacataaat
     6361 attagtaaaa aagaaataga aatgaaaagt acatcaaaca atgatgcagt agaaaataaa
     6421 ttaaaagata ataataaagt aagtaaaagt tatcaaaata actatacaga agatgagata
     6481 caagaagatg ttttaaatga aaaaactgat atggaaaaag atagtctaag tgaagaaaat
     6541 gaacatttac caataagtaa gcagagtatt atgagtgtaa atttagaaaa attggactta
     6601 cttcaaaata tagttggaga aattgtaatt atggaatcaa tggttgtaaa tagcgtaaat
     6661 tctacaggtg ttgatataga taatttcaat aaggcaacaa gacaattaca caagcttaca
     6721 gatgaattgc aagatatagt aatgtctatg cgtatgattc aaattgctgg tgtatttcaa
     6781 aaaatgcata gaattgtacg agatatgaat aaaaaattag ataaaaatgt agagttaata
     6841 actataggag cagaaacaga ggtggataag agtgtaattg ataatttagc ggaacctttt
     6901 atgcacctta taagaaatgc tatggaccat gcaatagagc ctattgagga gcgtatagcg
     6961 aaaggtaaag aagaagtggg aaagatagtt ttatctgctg aaaatctagg cggagaagct
     7021 gtgataagta tttcagatga tggtagaggc attatgagag ataagatttt ggcaaaagct
     7081 aaggaacaag gtcttcttaa acatcaaagt aatgagtata ctgatgacga ggtcaattct
     7141 ttgattatga ctccaggctt ttctacaaaa gaatctataa ctgaattttc tggaagagga
     7201 gtaggcatgg acattgttcg taaaagtatt gaaaaagttg gaggttcaat tgaagtaaaa
     7261 agtaaacagg atgaggggac aacctttact ataaaaatac cattaactct ttctatagta
     7321 gagggtatga agtttaaagt tggagattca ctatttactt taccaatatc atccataaga
     7381 agaacattta aacttacaga cttaaatcaa ataattacag aaggaaataa gacagagtta
     7441 attacaatat atggagcatg tattcccatt ataaggttac ataaaatata taatattcaa
     7501 acaaaagtaa ccaatctgat gaatggtaat atgataatta ttgaaaatga gcataaagca
     7561 gtatgcattt ttgttgatga aataataggt aatcagcaag ttgttgtgaa accatttcca
     7621 gtttatttta accgatttaa tataaaaaat aagggtttat cgggatgtac aatacttggg
     7681 gatggaagta taagtttaat tatagatgca gatagcatta tagaaatata ttaggggatt
     7741 taaagtatgg ctaaagatga aattattaaa caagtattaa cattttatgt caatgatgtt
     7801 atttatggaa tagaattaga aaatgtgatt gaaaccataa gatttcaatc aataacgtat
     7861 gtaccatgtc ttccaacata tataagtgga gttattaatt tgagaggaag aataatacca
     7921 gttattaata tgcatataaa gtacaatctt cctaaagctg attataatga gagaacttgt
     7981 ataataatta caaaggttga tgagtatcaa gttggaatta ttgtagataa agttgttgat
     8041 gttattcata tagaaaatct aaatttacta gagacgacta attctaacaa cactaatatt
     8101 aataaagata taaaagagat tgcgaaagtt gaagataatt ctatattaat tttagatatt
     8161 cgaaagtttt tgattaatag catatagtta gttctgataa attagcagga ggaagttttt
     8221 gcatgataaa actcacagat gaagaattta tagttcttgt aaaccatgta aaaaaagaat
     8281 atggtattga tttaagtaaa aaaagagcgt taattgaagg tcgcttatac aatacaatga
     8341 tagagaaaaa acttagttct tttagtcaat atatgaatct attatttaaa gataggacag
     8401 gaaatgaagc tataaaccta ataaatagat tgagtactaa ccacacattt tttatgagag
     8461 aacctcaaca ttttgagttt atacagaata gtatacttcc tttttgggaa gaaaataata
     8521 aaataagaaa cataaatata tggagtgcag gatgttctag tggagaagaa gcgtattcaa
     8581 tagctatgac tttggatgat tattttggat ataataaaga gttgtggaac attaaaatca
     8641 tagccacaga tatttcaact aactcacttg aaaaagctaa aagaggtata tacatagaat
     8701 caaatgttaa caatgttcct gagttatgga agaagaagta ttttatagac aacaaggatg
     8761 gtacatttaa aatttgtgat aagattagaa aaagtgtaat ttttaagccg tttaatttaa
     8821 tgaatgattt ctcatatcaa cattttgatt tgattttttg tagaaatgtt atgatttatt
     8881 ttgatttaaa aacaagagaa gaacttataa ataaatttta taatgctacc aaaaaagata
     8941 gttttttgtt tgtaggacat gcagaagtaa tcaacagaag tagtactaaa tacaaatatg
     9001 taaaacctgc tatatataaa cgataaaaag gtatttttga tatatgattt tttggaggcg
     9061 gtaatgtgga acgttttaat aatatggtta ttgctatagg cgcatctgta ggtggtacag
     9121 aggcaatact tgaaattata aaagacctac caaaatcaac accagggatt gttgtagttc
     9181 aacatatgcc agctatattt acttatatgt atgcacaacg gcttgacaag cagtgtatta
     9241 tgaatgtaag agaagctaag aataatgata gagtagaaca aggaaatgtg ttaattgcac
     9301 ctgggggtta ccagatgaaa ttgtgtacgg ataaacaagg atattatgta acttgtgaaa
     9361 aaggagaaag aataagtggt cactgtccat cagtagacgt tttgtttgat tcagtagcag
     9421 aagtagcagg taaaaattca ataggaataa tacttacagg tatgggttat gatggagcaa
     9481 gtggactttt aaaaatgaaa gaaaatggtt catttacaat tggacaggat gaaaattctt
     9541 gtatagtata tggaatgcca atggtagctt ttgatagagg ttctgttatg ttacaattac
     9601 ctcttggtga aatttcaaac tgtttgataa gacatattaa tattttaaaa caaaaagatt
     9661 aattttttat aaataaaaat taatctttta ttaaattata tggataaaaa accctagaaa
     9721 ctccaaatat aataagagag atttatattt aggaggaaaa tgataatatg aaatttttta
     9781 aagtcgatac aaaagctaaa gttttaagat atgtatttgt tttaacattg actataatgg
     9841 ctgttttggc aattaaacat gactataaat ataataattt aaggaaagat gcttcaatta
     9901 atttaaatag taataaggaa gcagtgactg ttatgagtaa ccagtcatta ccaaagggta
     9961 atacaaatag taaacaaact ttatcagtaa gtacaatagg aagtaaaaca atagtgaaaa
    10021 atgatgagta tttagaaagt actataaaca tgcctgtaat aacaaattca aataaaattg
    10081 ttgaaaggtc tacaaatgat aagataaaaa atgacatatt tgaattttat aataaatcat
    10141 ataaagaagc aaagcagtat cttaaggata atcctgacga gaaaaataag tttgttgcaa
    10201 atgtagactt tgaacttaaa aagaatactg attctgctct tagtataaaa gtgagatatt
    10261 atacttatag tggtggagca catggatttt atcaagatat agcatataat gtggatatga
    10321 gaactggaaa atttttagaa ttaatggact tgtttaagga taatactaaa tataaagaag
    10381 ttatagatga agaaattaaa agacaaatag ctgaacttga aaaaaaggat gaagaaaata
    10441 tcggaatata taattttaaa ggtataaaag agaatcaaaa cttttacttg caagatgaga
    10501 atctagttgt atattttgat ttgtatgata taactccata tgcagcagga ataccagaat
    10561 tttcaataaa taaagcgttg ataagttcta tgctaaaacc agagtatgta gatttatttg
    10621 atttgaaata gaagattaaa ttaaatgtta tgctataata tttagcatag agacttatta
    10681 aaattggggg tataaaaatg ctacttttaa aaaaagacga cataaaaaaa gtatttacaa
    10741 tgagagatgc aattgaagct gataaggaag cttttagaat ttattgtgaa ggaaagagtg
    10801 taaatccttt gagaaccaat atatctgttc caagtcaaga tgctagtatg ttatttatgc
    10861 caggatacgt agaagaatta ggctgtgggg gaataaaaat tgtatctgta tttcctaaaa
    10921 atgctcaaaa aggcaaacca gtaattccag caacagtact attattagat ggagaaactg
    10981 gagaagttag tgcagtattg gatggtactt atgtaactca gattcgtact ggagcagcct
    11041 ctggagcagc tatagacgta ttggctaaaa aagattctaa gattggagct ttgataggtc
    11101 taggaggaca aggtgagcca caacttgaag ccatgataga agctagaaat ttggatgagg
    11161 taagagtttt tagtagaaac aaggaaagta gagaaaaatt tgcagaagaa atgaacttaa
    11221 aattaagtaa atataatact aaaatagttg cagttgaatc atctgacgaa gccatagata
    11281 acgcagatgt gatagtttta gctactccat caaaacaacc agtacttaat ggaaacttag
    11341 tgaaaaaagg agctttaata agtgcagtag gttcatatat gccagatatg caagagttgt
    11401 gcccaaactg tttaactaga gcgtctaaga tattttttga atcaacagat gcagttttat
    11461 ctgagtcagg agatatatta attccattaa aagaaggtaa gataagtaaa gatgatttta
    11521 gtggtgattt aggaaatgtt ataaacggaa ctattcctgg aagagaagat gatgatgaga
    11581 taattgtatt taaaactgtt gggataggtg tacaagatgt cgtaacagca aaaagaatat
    11641 acgataaagc aaaagaaaac ggtattggaa tagactggaa gtggtaaaat attcaaaata
    11701 attctatagt tagacaaatt tatgttataa tgttgataaa taaatttttt attattttaa
    11761 ttgagggagg atgtttgtat gaaaatggta aaaaagttaa tattatctac gattatggct
    11821 ctagttttag ttctaactat agggtgttct aatgcagaag aaaaagtaaa gtcgaatttt
    11881 gaagctaatt tagctgcatt agaaatgtat aagtatgatg aaaaaggaat ttctgatgca
    11941 ttaacagaag ctgaatataa acagattcaa acttataaca gcaaggacaa ggtttcaatg
    12001 attgctcaat tgacacctct ttatggtaaa gaaaatgctg aaaagatata cgaaacaatt
    12061 gttaaattaa ttcaaggtgt tgaaaagaca gtaacagtgg aatcttctag taaggataag
    12121 ataactttga aagttgtaag taaagcttta gatactagta aagtaatgag tgaagcgaac
    12181 actttagtaa aatcttatgt aactgatgaa aagaattacg ataaaaaaga aaaagaagtt
    12241 caagatgata tgacaaaaat gattatagac ctttacaata agaaaccaac taaggaagtt
    12301 acaactatga ttacatatac taaaaaagat ggtaaatggc aaccacctag taatatacaa
    12361 aagttatgtg aaggaatata tacaatttaa tttattttaa agacacaaaa ctatctttta
    12421 agcatataat aaaatgtttg gaagatagtt tattttattt tcaaagtata aattatattt
    12481 tataaatgat ttattttata aataa
//
LOCUS       DAYATN010000115        12484 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.115, whole genome shotgun sequence.
ACCESSION   DAYATN010000115 DAYATN010000000
VERSION     DAYATN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.115"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            43..1473
                     /locus_tag="ACGI8A_002863"
     CDS             43..1473
                     /locus_tag="ACGI8A_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0180853.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            1673..2257
                     /locus_tag="ACGI8A_002864"
     CDS             1673..2257
                     /locus_tag="ACGI8A_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HFH0180854.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(2361..3140)
                     /locus_tag="ACGI8A_002865"
     CDS             complement(2361..3140)
                     /locus_tag="ACGI8A_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFH0180855.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(3198..4454)
                     /locus_tag="ACGI8A_002866"
     CDS             complement(3198..4454)
                     /locus_tag="ACGI8A_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFH0180856.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGIAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            4712..5611
                     /locus_tag="ACGI8A_002867"
     CDS             4712..5611
                     /locus_tag="ACGI8A_002867"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFH0180857.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            5635..7083
                     /gene="ddlR"
                     /locus_tag="ACGI8A_002868"
     CDS             5635..7083
                     /gene="ddlR"
                     /locus_tag="ACGI8A_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HFH0180858.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            7166..7948
                     /locus_tag="ACGI8A_002869"
     CDS             7166..7948
                     /locus_tag="ACGI8A_002869"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFH0180859.1"
                     /translation="MSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSNPNKKGNIK
                     SQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGKSMPTDEPI
                     SFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEKIKLIDDYD
                     AVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDVTPKVSTVY
                     ADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            7974..9893
                     /locus_tag="ACGI8A_002870"
     CDS             7974..9893
                     /locus_tag="ACGI8A_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFH0180860.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGFIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(10135..10482)
                     /locus_tag="ACGI8A_002871"
     CDS             complement(10135..10482)
                     /locus_tag="ACGI8A_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HFH0180861.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(10469..12397)
                     /locus_tag="ACGI8A_002872"
     CDS             complement(10469..12397)
                     /locus_tag="ACGI8A_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFH0180862.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
BASE COUNT         4741 a         1819 c         1721 g         4203 t
ORIGIN      
        1 tattttaata tatcattaaa aatttaggag gaaaatataa aaatgaattt agatttggaa
       61 ttttatcaaa aaatattaga agcttcccat gatgaaatat gtgtttctga tgataaagga
      121 ataataatat attgtaataa agcatttgaa gaaaattatg gtctaaaaaa agaagatatt
      181 ttgggaaaaa atgtttcttt tttagaagac tctggatatt caacaaaaag ccccatacca
      241 attgtattaa aaacaaagac taaattttca ctggaacaag atactcaaac agggaaaaag
      301 cttatcataa ctgctacccc tatatttgat gaaaatggac acttagaatt tactgttgaa
      361 aattgtagag atataactga acttaataat ataaaaaata aacttgaaga cacaaaaaag
      421 caagtaaaaa aatataaatc tgaagtagaa acactataca gaactgcact tagaattgaa
      481 gatactgtaa ttatggacgg aatagttatg agaccaatca taaacacagt aaatcatgtt
      541 tctaaaacgg atgtaagtgt acttttattg ggtgaatctg gaacaggaaa aagctcactt
      601 gctagatata tacaccacaa cagcaataga tctaatggcc catttataac aataaattgt
      661 gcaactatat ctccacaact tcttgaatct gaactttttg gatatacttc aggtgctttt
      721 acaggtgcaa gtactaaagg taaagttggg ctcgtagaat tagctaatgg aggaactctt
      781 tttttagatg aaataggaga tataccacaa aatctacagg caaaattttt gcaattaata
      841 caggatagaa cttttactcc agttgggagc ttaaaaaata aaaaggtcga tataagaatt
      901 atatctgcta caaatgttga tttagtttca aaagtaaaag aaaaaaagtt tagagaagat
      961 ttatactata gattaaatgt tatagaaata aaattgcctc ctcttagaga aagaagggat
     1021 aatctagtgg aaattataaa atattatttt aataggtact caagtgactt caacctaaat
     1081 aaaactattt ctaaggaagc tatggatgct attgcaaatt atagattccc aggcaatata
     1141 agagaattac aaaatataat tcaaaaaatc ttgcttactt gtacagataa tcatataaca
     1201 atagatgact taccaaacat tcttacaaaa aatatacata ttaccaataa tggaaataaa
     1261 acacatatat cccaaattaa taaggttatt atatctgact caaaatctac taattataaa
     1321 aataaaaatt ttgacacttt aattaaagag tatgaaaaaa atataatttt ggatgcttat
     1381 gaaaaatttg gtagttctta taaagtagcc aaacaccttg aaatctcaca gtctaaagcc
     1441 aataggttaa ttagaaagta tactaatact taattttaaa tttgataaat aaaaacttaa
     1501 cactatttac atattgtaaa tttggccatt tactgttatt atgaatatgg ttgaaattat
     1561 ttaaataaat actgttgact tagtttcata tatttaccat taaactttta tcataaatat
     1621 catatcataa taatatattt aataagtcta ataaactata cgaggtgtaa atatgttaat
     1681 gaatacccat tttattatgg caaaatcaat acttgataat atagatgaaa ataaaacatt
     1741 ctttataagc gaaaaaaatt ttatatatgg caatataaag cctgatgctt tttctaaata
     1801 taaattaaaa aaacattatt tagatgagtc atttaatatg attgtttcta aaataaacta
     1861 tctatgcagt cttaatatag atagtctttc taaatacttt tcagtaagta gacttagtca
     1921 ggaactagga gtcatatgtc attttttatg tgattttttc tgtgtagctc acagtgaacg
     1981 ttgggaattt aagcatagct taaataaaca tgttacttat gaaagagaac ttggagcatt
     2041 tgcaaaagat gttgatttat ctaaaattaa atgtggtact ataaatagta gttttgagaa
     2101 gttttttact aaactttata gtgattacaa aaagaaaact gattatatga atgatttaaa
     2161 attttcagct tatacttgta atagtgtcat aaattatata cttgactcta ttttatctaa
     2221 tacaataaaa tcatatcata tagcaaattg cggttaactt aagaattttt aaacttatat
     2281 aaacttataa ttgaatataa ttaaaatgac tgctctatag aatataattt tatacttctc
     2341 taggacagtc attttttata ctaactattt atctccatta taacatctat ggcacttttt
     2401 acactatcta ctacataatt tgcactttgc tttacatctt cccttgcctt agacattgca
     2461 aaactatatt taaatccttc taacatgcaa atatcatttg gagaatcccc aatagtagca
     2521 acttcatctg gacttacatt taacgttttt attacatgct ctatacttcc tctttttgac
     2581 acacttttag gaactatatt taaagtatat gttccagaaa aatatatttc taagacttca
     2641 ctaaactcac tttttagata aagttctatt ctggataact cttccaaatt ttcagaacat
     2701 acgcctatat ttcctataag attatcaaaa ctacttcttt tttcaaaatc tgggtcaact
     2761 ataaagtcac tagaataatg tctaaaagca gtggtatcac tacccacaat tacatcaccc
     2821 ttatatttaa aataaagttg tgcatcactt gactcaaatc tatcaatgac tttttttact
     2881 aaattatcat ctatcgttct tttaaaaatt acattataat ctttatcata tataaatgaa
     2941 ccattttcac atacatagta tccattcata tctaaactat ctttgataat attatgtgct
     3001 tgtttaaaaa ttctacctgt agcaacatta aactgtactc cctttttttc tatttcttca
     3061 attgctacta tatcttcttt tgttatggtt ccattttcat atagagttcc atctaaatca
     3121 caaaatatat gttttatcat aaattaaaag cctccaaaaa caattttata ttttactaat
     3181 actttaatca aaataactta aagcttttct aattgtattc tcaattcatt aagcaaatct
     3241 ctagtagctc ttaatcttat ctttcttcta tccccattaa atatattttt ctttactatt
     3301 gtttttccat ttatatatat tgcagtataa accagtccca caggcttctc tttggttcct
     3361 ccaccaggtc cagctactcc agttgtagat atacctatat tagctttaag tcctttagca
     3421 atgccctcag ccatttctat agctgtttgc tcacttactg ctccaaactc ttccaaagtt
     3481 tctcttttta ctcctaatct tttcatttta gaatcattag aatatgttac acaaccttca
     3541 agaaatacag aggatatacc tggatagttt ataagagatg aagaaaccaa acctccagta
     3601 caagattcag ccactgctat tgttagattt ttttctacaa gtagcttaga aacagcatct
     3661 tctaaagcag tctccccttc agatatggaa atatctcctt cagtatatac atatttacca
     3721 actctacttt ttattttttc ttctactggt tttattaatt taaaagcttc tctttcattt
     3781 tcagcctttg cagttattct tattgtaact tctaattctt ttgcatataa agcaactgtt
     3841 ggattagtct gctcattaat tatatcgaga atttctaatt ctagatttga ctcacctatt
     3901 ccatataatc ttaaagtctt agatactagc atttcatttg tgaattgttg taagtaaggt
     3961 ttgacactct cattaaacat agctttcatt tctcttggag gtccaggcaa tactattata
     4021 aatttaccat ctttttttaa aattgcccct ggagctgtac cattattatt ttttagtact
     4081 atagcatctt ttggaaagta cacttgtttc atattatttt cggccatttt tttccctgtt
     4141 ttaacaaaga aagattccaa aacctctagt gaaggtttat gtagttctaa tttctgacca
     4201 aagtattctg cagcagtttc ttttgtcata tcatcaccag ttggcccaag accacctgta
     4261 gtaattacaa aatcacttct ctttaagctt tcatcaaaac attctaaaag tctttgtttg
     4321 ttatctccaa cagtactctg atggtatact tctattccca atgatgcaag ttctttagct
     4381 aaaaattgag aatttgtatt tactatatct cccaataata tttctgttcc tacacttatt
     4441 atttcagctt tcaaaattta tcactccttg atttatagcc attaagacta gtcttaagtt
     4501 cataacaata attttagcaa aaataataac ataaataaag ttaaaaaatc atttaaacat
     4561 atctttaaac tagattatta ttctaaaacc acacataata taaacttcgt atatagtggt
     4621 actttcatcc tcaactgtta attcatataa ttaagtatgt aaataaaaag tttactttaa
     4681 aataataaaa attaaaatag gagtgtataa tatgaaaata gcagttataa tgggtggaat
     4741 atcatcagag agagaagttt ctttaaattc aggaaaagag atatataata accttgataa
     4801 aaataaatat gaagttgtaa aggtaataat agatgataaa aaagatatat ttacaaagat
     4861 accagaagat atagattttg caatacttgc tttacatgga aaatttggtg aagatggatg
     4921 tatacaatca atacttgaga ctatggatat accatactct ggatgtggac ctctttgcag
     4981 tgggatgtgt atggataaaa atataactaa aaaaatgctt agagattcaa acctacctac
     5041 tgctccatgg gttttagtaa aatcagttga tgaaatagac tatgatgaaa tagataatat
     5101 aggttatcca gtatttataa aaccaaactc aggaggttca agtgtagcca cattctttat
     5161 tcattctaag gatgaagttg aagaagcagt tagaaaaggt ctggaagttg atgaatttgt
     5221 aatgattgaa aaatatatac caggtggaga atatacttca ttcatattaa atggagaagt
     5281 gttccctaca atatctataa aatctgattc aggattcttt gattatgaag ctaaatactc
     5341 agttgaaaaa ggtgcaaagg aagaagttgt ttacttagat gaagaacttc aaaaaagagt
     5401 aaacgaaatt tctgagactt gttggaaaat ttttaattgc aaagcatatg taagagttga
     5461 tatgattata agtgaaggta ttccatatgt tcttgaacta aatactcttc ctggaatgac
     5521 tcaaactagt ttaattccta gaagtgctgc tgctagaggt attaagtaca gtgaactatt
     5581 agataaactt atagaatact ctttaaatta gatgaaataa tttgaggtgg taatttgata
     5641 ttttcaaatc taaaattaaa tgataatgaa ccaatttata tacaactaaa aaattatata
     5701 agtgatatga tatcaaaagg actaattcca gacaatagta agctcccttc tacaagggag
     5761 cttagtcagc ttttacaagt tagccgaaat tctgtagttt taacttatga agaattaaaa
     5821 tcagaaggat taatctactc tatttcagga aaaggtacat ttgttaaatc aaagaataag
     5881 tcttcaaata ctacatggtc attaaactgg gattgtttag agaatacata ctctaaaaaa
     5941 gccaatgaat tggacattat aaaaagtgaa attccttgga gctctgacct gatttccttt
     6001 aaaagtattt ctccagatgg agatttattc gacatggaag aattaaaaaa atccttttta
     6061 aatagaattt cactagaagg acataaatta ctcaactatg gatatgcaca aggttataag
     6121 ccacttattg actatttact agaatatatg acaaacaaag gtgctgatat aagtaataaa
     6181 gatatactaa taacaaatgg attcacagaa ggctttgata ttattatgtc atcttttacc
     6241 caagaaaaag actacataat atgtgaaaat cctactcata atactgctat aaaaattatg
     6301 aaatctcata acctcaatat cttaggtgta gatataaatg aagatggttt ggacttcaat
     6361 atgctagaag acaatttagt taagtataaa aataaaataa agtttgcata cattacaccc
     6421 tcttatcaca atcctacagg aattgtaatg actcctgaaa ataggtacaa attctataat
     6481 cttatgaaaa aatacaatat agcaattata gaagatggat ttaatgaaga attactctac
     6541 aatagttctc atatatttcc tatctgttcc ctagataata tgaataatgg ggtcgtatat
     6601 attggtagtt tttcaaaaat tttatttcct ggaatgagaa ttggatggat atttgcagat
     6661 aaaaaagtaa tcaataagct agaaagtgta aaaaggtgca gaaacataca tgtatccttt
     6721 ttagaccaag gaattttatt tgattattta agtaatggag gttttgaaaa atacattaaa
     6781 aaaatacgta aattctatgg agacaaattt aactttgcat ataaatgtat aaaaaaatat
     6841 ataaaaacag aatatatctt gggtgatggt ggtttgcata tatttataaa actaaaaaat
     6901 atagatacta gagttttatt agaaaaatgt tatgaaaaaa atgttatatt tatgcctgga
     6961 gacttatttt ttacagataa ctctggtttt gatactttaa ggctcggatt ttctaggttg
     7021 tcttttgaag acattgaaat tggaataaaa ataattggag aaactataaa tgaaatgttc
     7081 taggttaact aaatattgtt agcctagttt atttttatga ggtataatat atatagttta
     7141 tatgaatcta atactggggg ataatatgag taaaaagtca aaacgaagaa aaattaataa
     7201 attaaaatta acgattgtaa taactgtatt atttcttgct ttattatcta tatatgagat
     7261 actgttctct aatcccaaca aaaaaggtaa tataaagagt caagaaacta tgcaaaataa
     7321 aaataataca acagatgatt ctaaaaataa tcaaagctct accaaaaagc aggaagataa
     7381 taatatcaaa cctacttttt catattcttc aataccagat aatataaaaa ataaaatgat
     7441 tggtaaatct atgcctactg atgaacctat aagctttgat agtctatctt atttaaaact
     7501 tacatattat ggatttgatg aaaaaactca tcaaggagaa atgatagtaa acagtgaact
     7561 agctccagaa gtagtggata tttttaagga attatacgaa aaaaaatatc caattgaaaa
     7621 aattaagtta atagatgact atgatgctgt agatgaaaaa tctatgtctg ataataatac
     7681 ctcatcattt tgttatagaa ctatagctgg tacaaatgta gtttcaaatc atggtaaagg
     7741 acgtgctata gatataaacc cattacagaa tcctcaagtt tctggaaatg atgttacacc
     7801 taaagtaagt acagtttatg ctgataggtc tagcacaaaa tttggaatga taaaaaaagg
     7861 tgatgattgt tataatgctt ttgtaagcag aggttggagt tggggtggct attggaaaaa
     7921 cccagattat caacattttg aaaaataata gattagaaat aaaggtgaat tatatgaatt
     7981 taatgacatt ggaaaatata agtaaaagct attctgaaaa aaaattacta gaaaatatat
     8041 ctcttggaat aaatgaaggg gaaaaaatag gatttattgg tgtaaatggt actggtaaat
     8101 ctaccttact caaaattatt gcaggagttg aagaaagtga gactggtaat ataacaaaaa
     8161 ctaatggtat taggatagaa tatctacctc aaaatccaac ttacaatgaa aattcaactg
     8221 tacttgaaca agtatttaat ggaacttctg aagaattaaa gctacttggt gaatatcaag
     8281 aaatcctaga aaaacttaat tcaaacttta cagaggaatt aaataaaaaa ttattatcag
     8341 tacacgaaaa aatagatgcc ttaaatcttt gggatttaga aagtgaagct aagtcagtac
     8401 ttacaaagct tggtataaat gactttaatc aaaaaatagc agaactttct ggtgggcaaa
     8461 gaaagagagt ctctctggca tcagccctta ttacaccttg tgagtttttg atactagatg
     8521 agcctacaaa ccatctagac aatgatacta tagattggct tgaggaatat cttaattcca
     8581 gaaaaggttc attgcttatg ataactcatg atagatactt tttagaccgt gttacaaata
     8641 gaattattga actggacaaa ggtagattat tcagttatga tgggaattat tcaatatttt
     8701 tggaaaagaa aatggaaaga ctttcgctag aagcaagtat tgaagataaa agacaaaatt
     8761 taataagaac agagcttgcg tgggtaagac gaggagctca ggctcgtagc actaaacaaa
     8821 aagctcgttt acaaagattt gatgaactta taaatagaga ttcttttaaa cctgatgaaa
     8881 atctcgatat atctgttgca tcaagtagac ttggaaataa aataatagaa atacacaata
     8941 tatctaaatc atttgaagaa aatactgtta tagataattt agaatacact ttaactcgta
     9001 cagatagaat tggtataata ggcaaaaatg gtatgggtaa atctaccctt ataaatatac
     9061 ttaatggaga aatagagcct gatagtggat atatttcaat tggagaaaca gttaaaatag
     9121 gttgtttttc tcaagacgac tctcatatga atattgatat gagggctata gactatgtaa
     9181 aagaagctag tgactatata gaaacttcag atggtactag gattacagct tctcaaatgt
     9241 gtgaaagatt cctttttaat ggtactatgc aatatacttt gataggtaaa ttatctggtg
     9301 gtgaaagaag aagactacat cttcttaaaa tccttatgac agctcctaat gtgcttcttt
     9361 tagatgaacc tacaaatgat ttagatattg aaacgcttaa aatattagaa gaatacctag
     9421 acgaatttaa aggtgtagta gttacagtat cacatgatag atatttctta gatagaattt
     9481 gtaataaaat atttgcatat gaaggtaatg gaaaaattca tatctataca ggtaattaca
     9541 gtgattatct tatttataga gagattcaag gaattgaatt tgaagaatct aaaagggaat
     9601 cagttaaaaa agatgactct actaccccaa aaaaagaaaa acctaaaaat gataaaaagc
     9661 ttaagttctc atataatgaa caaagggagt ttgaaaatat tgactctgat atagaaaaac
     9721 tcgaatctaa aattgcaaca cttgatgaaa gtactgcaaa attttcaact gattttacta
     9781 aactccaaga aattttagat gagaaatcta aactagaaaa agaacttgaa tacaaatatg
     9841 aaagatggga atatttaaat aatctagctg atgaaatagc taataataag taatattaaa
     9901 ataatcttaa gtttaataag ttttgcttta aatctgtaat ttataaatat tttaaaggaa
     9961 gtgtctcaaa atgaactttt tagttttatg agacactctt ttttatgaga aatcaaatat
    10021 attttcatca tttcaacaat acaaaagagg cttatctcta ttttgagaca gcctctttca
    10081 taattacctt ctaatttata tgctctaata tatctataat aattcttatt atatctatat
    10141 atccagtctt tcaatatagt gacaattaac ttcaatatga tttaaaaata ctaacaacct
    10201 ttcataaagt ggatgagatt cttctccata tttagcttct aggttcttgc ctatttctct
    10261 aacagtcctt tgaccatcaa tttgtaaaaa aacaaaactg gaatattcat ctagttctat
    10321 ttttttgtat ttaggtattc tagcttttaa ctttctaaaa aacttttgaa ttttatggtc
    10381 ctgtttttca agtatagtaa caacacgatt ttcagctact tcatattcaa gtccatcagt
    10441 aattttaaat acaatatcta aaacatcatt actcttgctc attatttttt actttagata
    10501 aaacaatagg taatgctgta agcacaacta aaactactaa taaagcaatt gccattgaat
    10561 ttgttgcagc aaatccactt ggtacctttg gagttacaac tccagttact tgtaatatta
    10621 tacctattaa accaatgata gaaccaccag caacaagacc agaagataaa cttataccat
    10681 tagaaacttt aacttctttt tctttttcag ttttagatac taattcaaca aataaacgaa
    10741 ttaatgctcc aactaatatt atagaagtag ttgcaattgg taaatagaat cctatagcta
    10801 tagtcattat tggtaaattt aatgcatata gaactattgc cataaatact ccaacaataa
    10861 tcataaccca aggtaaacta cctgacataa taccagaagt taatgttgac attaagtttg
    10921 cttgtggtaa tgcaaattgt acattatcac cagttgctct aagttggtca gaaagtaata
    10981 aaattactcc tacaacaact ataacaccaa ctatacttgc aattgtaaag taacgttgca
    11041 tttcgttttt gctaccacca ataatataag ttactttttg agattgagta taacctccag
    11101 ctatagcaat agcaacaact ataaatgaac caaataaaag taatgattta ttagcttcta
    11161 aatcagtcca tcccattata acaaacacta atgttacaat aactagagaa gctatagtca
    11221 taccagaaac tggaagattt gatgttccga ttgttcctgt taaacgacca gctactatga
    11281 caaataataa agataataaa agagatatga tagctccaat aattgccata acaatgttac
    11341 cagaaattaa gaatgctgct aaaaatccaa taactactcc acctaataaa agtatcattt
    11401 gtatagatga accttcttct ccttctccag cacttgattt agctttaagt gtttctttta
    11461 tagaagcgat tatagttggt ataagtttta ttgcaccaat aatacctcca caaagcatca
    11521 taccagcacc tatatatttt acataactac tagatattgc tccaacatcc atttggttaa
    11581 gaggcattgc aggattattc cataccatag ctccatcttt agccatatct gtaaagtatc
    11641 ctattaaagg tgctatacca aagtttgata aaatacttcc tgcaaacatt gttaaagaaa
    11701 cttcaagccc tactataaat ccaattccta aaagtagagg atttacttca atatcaaatt
    11761 tccatttgta gaatttgctt cctacaagac tcattacatt gtttgctaca tttaagaatg
    11821 aaccagttaa tacagtaata aatccactta ttacaaatcc aattcccatg tacttaattg
    11881 aatctccacc agcctctgaa gcaacaagtg tctcagatat agccattgat tctgggtaca
    11941 taagtttacc atgttcttca actattagat agttgtgaac tatagcagca attcctatac
    12001 caaataaaac tccaccaact ccaacagcta agccttctaa gaatgtaatt tggcttccaa
    12061 ttaatataac tgctggtaat acgaaaataa atccacttgc aacagattct ccaccacttg
    12121 acataccttg gattaagttt ttaccaagaa taccctttga acgagcaaat gctccaacaa
    12181 aagcagaacc tattatagct cctggaatac ctgcggcaac tgtaagaccg gctttcattc
    12241 cagagtaagt tgtagatgcg gcaaaaatgg cagctaaaat aataccgata atcagtacag
    12301 ctacatttcc accggttctg gacttatctg tgatatatgg aacgtaatct tttccactaa
    12361 cctcaccata agcaccctta ggtaattttt tgttcataaa attttcaccc cttttcaatt
    12421 tgtatatatt ataacatata tacatataaa acttaacttt ttattatttt acaaatagcg
    12481 agtc
//
LOCUS       DAYATN010000116        12135 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.116, whole genome shotgun sequence.
ACCESSION   DAYATN010000116 DAYATN010000000
VERSION     DAYATN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.116"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(78..521)
                     /locus_tag="ACGI8A_002873"
     CDS             complement(78..521)
                     /locus_tag="ACGI8A_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180863.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(524..721)
                     /locus_tag="ACGI8A_002874"
     CDS             complement(524..721)
                     /locus_tag="ACGI8A_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0180864.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(913..1779)
                     /locus_tag="ACGI8A_002875"
     CDS             complement(913..1779)
                     /locus_tag="ACGI8A_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0180865.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(2005..2874)
                     /locus_tag="ACGI8A_002876"
     CDS             complement(2005..2874)
                     /locus_tag="ACGI8A_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0180866.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(3380..3601)
                     /locus_tag="ACGI8A_002877"
     CDS             complement(3380..3601)
                     /locus_tag="ACGI8A_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180867.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(4102..5082)
                     /locus_tag="ACGI8A_002878"
     CDS             complement(4102..5082)
                     /locus_tag="ACGI8A_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180868.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(5246..6118)
                     /locus_tag="ACGI8A_002879"
     CDS             complement(5246..6118)
                     /locus_tag="ACGI8A_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0180869.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANSKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(6282..6833)
                     /locus_tag="ACGI8A_002880"
     CDS             complement(6282..6833)
                     /locus_tag="ACGI8A_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0180870.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(6853..7755)
                     /locus_tag="ACGI8A_002881"
     CDS             complement(6853..7755)
                     /locus_tag="ACGI8A_002881"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFH0180871.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(7770..10142)
                     /locus_tag="ACGI8A_002882"
     CDS             complement(7770..10142)
                     /locus_tag="ACGI8A_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HFH0180872.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(10363..11052)
                     /locus_tag="ACGI8A_002883"
     CDS             complement(10363..11052)
                     /locus_tag="ACGI8A_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0180873.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(11293..>12135)
                     /locus_tag="ACGI8A_002884"
     CDS             complement(11293..>12135)
                     /locus_tag="ACGI8A_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0180874.1"
                     /translation="VNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGASSIFL
                     ILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIYCIDNL
                     RNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEVCLKKGIKFTCSINFSK
                     VNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITRSFFVLKCENSKLNQ
                     LTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPLDQDTD
                     SWGD"
BASE COUNT         3972 a         2138 c         1156 g         4869 t
ORIGIN      
        1 tatataaatt tatcttctag cattaattta aaaactaatt tatttttata taattttcta
       61 ttttatatca taacttatta caacttttta tctaatttat tgtatacaac aacactacaa
      121 ataaattgaa ataaaataag caatccaaga acaactaaaa cccacttgct tattaccatt
      181 acagaaaata cactcaatat agaaacaata aatataagaa ttacaagaac ataggacatg
      241 atttgattag tgatacgtcc agctttatca cgtaacataa ttcgtctttc atcattcatc
      301 tctatccttt gattttttat cttttcttca taatctactg ctctttcagg tttactccaa
      361 tacatatatt gaagaatcat acatacacct ggacctaaag cagctccaga taatccccaa
      421 ataaaagcct caaatgaaaa ctcagtaaat gtagccaata aaacaagtaa aattccacaa
      481 ataaaatata taattccaac atataacata ttctttttca tttttactcc tcctcataaa
      541 taaatatttc ttcaatactc atatcaaagt atctagctat cttaaaggct aagatgatag
      601 aaggattata cctaccattt tctaatgaac ctatagtttg tcttgatact tgcaaaatag
      661 aagcaagttc ttcttgtttt atgcctcttt cttttcttat ttcttctaat ctatttttca
      721 catctatccc cctttaatat ctcaactacg gaaagtctac tttccataat tatacaacta
      781 tattccattt atgtaaagct tactttccat atttacaaaa aaataacaga ctattttcag
      841 cctgtaattt tagaatttca tgttgtttat taattttaat gttatttatt aattccatat
      901 tatttattaa ttttaacttg gtttctgaat tggaatccaa acttctgtag tataatcagg
      961 agcattgata tcgccattgg gataaagttc aatatctggt aaattagcat acacataccc
     1021 agaacttggt aaccactcag taataattct tttttgcaat tcctgtattg ccagagcatt
     1081 tggcattgga gaaacacatt caaatactgc ccatgtgcct gatggtatta agtattcatg
     1141 agtatcttct aatacaggct tatctgttgg agcagcaata taataatcta aacccttact
     1201 atttgtacac acactaactc caagcaatcc acaaggttca ttatttatta actctgaaag
     1261 tttatcaatt gttccatttt ttatgcattt agcccacaac ttaggaactt tctcaaagca
     1321 ctcttctaca atcatgttca tactttcttt tataccaaca atacgaaacc ctttcttttc
     1381 tacaatacga tattgcattt ctatatttcc ttttacataa atgcttaatg tcatcttagg
     1441 atatgctttt aaaacaacac cctttgctct agctgcagat ggagaaatgt tatgtatatt
     1501 ttgaaaagct ctattaaaag atgttggtga atcgtaacca tacttctcag atagttctaa
     1561 aattttgatg ttactgttct gaagttcaaa tgcagcctta gtcatacgtc ttcgtctaat
     1621 atattcagat aatggtactt ctgcaatata tgaaaacatt ctttgaaaat gaaaggttga
     1681 acaacatgca atttttgctg cttctgcata atccactcca ctttcgagat tttcttcaat
     1741 ataagtaata gcttgattta atttatttat ccattccatt tgatattacc tccaagcaaa
     1801 ttatacagtt taataaaata atatacctct ctattcatgc acaaaattgt aagcatttaa
     1861 atttataagc atttttcaat caatacaata aatataaaaa ttgtattata caataaaaaa
     1921 gttcaataac attatattat aaaatgttat tgaacttttt ataaaaatta atttaagtgt
     1981 agttaaatta agtattacac tatcttatat attacaatat cctactatct tatagtcttc
     2041 tggcacttta taatctttgt ctggagtacc taaattccat tttaaatcaa acatagttgc
     2101 acttattatt gttgcaaaat aaagcataac tataccaatg tctatttttt cttcatataa
     2161 attagtatga ccatcttttc taacagctaa aactacagtg cctccatcaa ctataaatct
     2221 ccatggttgt ctatttaatg tagatggagc taatcttgca tagtgaaatg catcaagaag
     2281 tgctctttct tctaagattt ctatacttgc aacatttccc cattcgttca tataaacaac
     2341 atcttctaca cctagtctag tagacccctt atctgttaca acttgcatat cagattttga
     2401 ataattttgc ccagtttttg tagcatttaa tactttagca ttacttacat tttctccata
     2461 tccaatagct ataattcctg ttacctcttt atctgaatta attccaagtt tttcttttac
     2521 cttttttcca tcttcaaaag taacccaaca agaaccaatt ccaagttctg ttactttaaa
     2581 gattatactt tctcctatgt accctgaatt ttcaatgtaa ttatctttta tgtcagataa
     2641 tattataata taacttggag aatctatcat atttcctttg taccctgcta ctttatctaa
     2701 attattaaaa acttcacttc tattcataat tcttacatca acatctattt ctggaactag
     2761 tttcttacaa gattctgagt atttcttaat ttcactaaga tattcttctt taatatctgt
     2821 gttcttatat tctctaacag actttctatt taatatgatt gttttataat ccataatttt
     2881 atcctcccca aactagtatt gtcttaatta tatacccatt aatatattat tttactcatt
     2941 tatttaataa ataaagatta caattttgta attattattt attaataata ttccactttt
     3001 acattaataa tgaccagttg tgcaaaatcc attgccaacc accaaataaa aacatattat
     3061 aaaggtaata taaaataaca aacaaatctt aacaaaaaat taaacttttt ttacacgaaa
     3121 gaaggcttcc cgttgccttc tttattttta taaaaaaata agctgtttct aaaatgattt
     3181 cacatttttg aaacagccaa gttattctat aatgacaact ataatttgag gatgtgaggg
     3241 agagttgtca ttataaattt agggtaatta tattatgttt taagctagtt ctttctatat
     3301 ttatataata cattttatat atgaatttta tatgagattg gtataaatga tatatgaaat
     3361 tacatatgtt tatattatat taaattttat tattacattt tccacaacaa tactcatatg
     3421 tctccacatt atttggaata tttacaaata caaattcttt acagttctca caatacttta
     3481 atatagatat agttccacaa gtgtaacatt caaatattac acaatcctca ttttcctcta
     3541 ctatttcaaa ctttttacta tgttcaccac actctgggca cttgtgatta tttacattca
     3601 taaatactcc tccttattag tatagttaca tgtcaaaaac ttaattaatg tattctatat
     3661 tacttattat atattctata ataaatcata ttctaatata ataaaacaaa ttaaataaaa
     3721 aaaaaccata aattttaaat ttatggatta attcttgtaa tgataactct atttgaggat
     3781 gtgagggagg gttatcatta caatagggta ttatattatg gtttttaagc tagtctttct
     3841 acacctcata tgatacatat gctatatgaa ttttatatga tttttacata aatattttat
     3901 gaaatttaaa ttttttagtc attttgaaat ttgtaaaaag ttctctaaaa atccacagca
     3961 tcataaacag ccaaaattta agtagaacat cataaaaaaa tttgtccaat aattttaaca
     4021 agtaatctca cctcattata attattgaca caaacttaaa taaacaatct ataaatcttc
     4081 tataaatctt aaactttccc cctattgata cacaatttct agtttgtcaa ttttattttg
     4141 catttctaag aaaatttctt caacagaatt gttaaatata ttgcctaaat agattctttt
     4201 atctttttga aatgaataag attttacact accatcagaa gaaatatata aatgactcca
     4261 ctctgctcca tattttgaaa acctctcctt tgtatcttta aacctcatat cttctatttt
     4321 tattgctttt tctctgtctc ctcttactac agtgtgaccc ctttttatta aactggttat
     4381 ttttatatct tcttttaatt taatacgtgt atcatcattt tttgaatata ctttccatat
     4441 cactttatga cgataattat ctaattcctt tattaagctt ctatcaatag taaaactatc
     4501 tttagttacc acaattaaat caaaatggtc taacatatat tgaaatattt ctctcatact
     4561 tttatttttt aaaatatcct caccaataac ctctataaat ctacagcctt tctcatacat
     4621 gcagttcaat aaacttgtga tttcagaaaa ataatcattt gtattattat ctccatctat
     4681 ttctatagta aagtacaaag gagtaatgta tgatttattt cctgttatct caaatacact
     4741 tgatagtgtc tgtatatcat cttcaaacgt taagtataca ttctcataaa acataaaaaa
     4801 ttccataact ttttctttca tattatggat atttctatat ctattggata atatatttat
     4861 tatatcgtca gtagtatttt tcccatcaca ttcagccagt ataacaattt gcttccaact
     4921 taagctatcc acatttccga tacttttttt atatccatct tcttcatcat aaataggacc
     4981 aattataatg ttttcttttg gccacagatg aaccctagtt ccatctttta atttcggata
     5041 tttatttttt acagactcat aaaatctact tgtatcaccc atgattcttt actacccccc
     5101 atagtttaaa tattctatgc taaaaatagc actttatttt tattaattca acaattattc
     5161 aaaattggtc attatatatg tttaaatgga tttaacatca accttattta tatgttattg
     5221 atgtttatat atgttatata aaatcttatt tctcatactt caatgtaatc ttaactatct
     5281 ctggtttaca attaagccta attaaaactt ttgagcctcc taagccttta tttaaaatca
     5341 tagtgctatt atctttttca tattttcccc agtcatactt aggaaagaac tctctatttg
     5401 gagataatac acctccaaca aaaggtattc ttattatacc tccatgaaca tgtcctgcta
     5461 aaaccaaatc tgctccccat tttgcatatt tttcaaagaa aaatggtgta tgtgctaaaa
     5521 gaatgttata ttcattttca ttaactttac ctagattgtc ttctacaaaa ttgtcactta
     5581 gttctaattt tttgctatta gcaaataaat atggatagca ttccaatggt ataattaaac
     5641 catataaatt aagatggcta ttacctctat atattcttac agattcattt tccatattta
     5701 ttattttctt tgaatatagt tccttaaaat aatccttata tagatgttta tactttttta
     5761 ttaaagattt ttgttcatga tttccaatta tatgatatac ttcatatttt tcaaccaaat
     5821 tatctattaa atctaatgca acttgaaaat ttttattttc tccatctact aaatctcctg
     5881 ttataaatat aacatctggg tttaatgatt caattttatc tatcaatacc ttattatttt
     5941 tatcaaacac cttattatgt aaatcactca cttgaactat tttaaagtta tcaaattcct
     6001 ttggaatttt tttgttgaaa atagtatatt ctgtcgtttc tatatgctga gtttcatact
     6061 tatacagaat atacaataat attaatacta atactgtcat tgaaaccttt ataatcatat
     6121 tctcttcctc tttattctat tttgttaata ttattataat gcttttatgt tttttttaaa
     6181 acaataacat catcacatac atataaatct tttcgacatt ataaaaataa gttatccttt
     6241 tctaaaattt tcatttattt tagaaaagga taactataaa attatctata aacctctata
     6301 ttgtgaaata tagtttttcc tttatttaaa tttttatata tatgctctaa agttccatca
     6361 aacctaatag aatgtccaga ctctaaaata taagttttcc catccacatt aaggtcaagt
     6421 ttaccttcta ttacgagtac atactcttct aaaacatgac tgtgtgtaga tgaaacatga
     6481 gtacagtttt cctcaagctc aattataaaa agttcaaacc ttctttgagg gctaaaaggg
     6541 tatattggat ataacttcat gttccctttt tgctcaacaa taggctctac ttcttctttc
     6601 tttataacct cataggtatt tgtattttca tttaatagtg tagtaaaatt tattttaaat
     6661 ccagatgaaa ttttccataa agtagatact gtcggacttg attcacctct ctcaatctgt
     6721 ccaagcattg ctttactgac acctgtcatc tttgaagctt tgtctaaact tatgcccatt
     6781 tcttgtctca ataaccttaa attttctccc acattaggaa atttattatc catgttaaaa
     6841 tcctcctgat agctatttgt attctatgat ttttactcct gtaagttcta ctatctcttt
     6901 taattttaac atatgattgt tacaaatttc atcaaattca tatatataat cttcaagccc
     6961 taatgcctta tatttatcga ttccaaattt atgatatgga agtaactcca tcttgcctcc
     7021 tggaacattt tgtttgacaa ataatgcagt atttcttata ttttcttcac tatcatttac
     7081 tccataaata attggcactc ttataaccat agatttattc aactttgaaa gcctacatat
     7141 attatctaat ataattttat tacttactcc tgtatattct ttatgaatat tatcatccat
     7201 agattttata tctacaaata tgtggtctat tttctcaaat acatcattta ccttattcca
     7261 atcaaaatat ccacaagttt caattgcaat atttatgcct ttatcataaa aaatatcaac
     7321 taattctctt aaaaaatcaa tctgaagtgt aggttcacct ccagaaaaag ttatccctcc
     7381 attagattca aagaaaaata taaaatcttt ttcaacttcc tctactattt cttcaacact
     7441 catttcttcg gtcattatac aaatggcatc tttaagacat accttgatac actctccaca
     7501 taaatcacat ttattattta tttgcgctct atcaaatagt gaacttatat tttgaggaca
     7561 aacatctata catctattac aggatacaca tttatctttc ataacaccta attttactat
     7621 gttactccag gaatctggat tagaacacca cttacatttt aatttacatc cttctaaaaa
     7681 tatagtagtc cttattccat ctccatcatt tactgaaaaa tgttgtactt ttacaactct
     7741 acctttttta ctcataatct acctcataat taaaaattgc tatgtgaatt tctagctatt
     7801 atagcatctt gcatttctct tgataaatta acaaattgag tactatatcc agcaactctc
     7861 actaataaat ctttatattc ttctggattt tcttgagctt ttaatagagt atcatttgat
     7921 atcgtattaa attgtacatg gaatcctcct aaagcaaaat atgatttaat catagatgtt
     7981 agatttctaa gtcctctttc agtttccacc aaatcttgat tcagtcttaa atttagaagt
     8041 gttccaccag tatgaacatc atgatttatt tttgatgcag atttcattgc tgctagtgga
     8101 cttgttgtat cagtaccaac aaatggagaa actccctctg tcaaaggttt tgtcgctttt
     8161 cttccacaag gagttgcgcc tacgattttt ccagttggta catagtttga tatacccata
     8221 aatgcactat taaatttaga gccaaaaata tccttgtatt ttctagtttc agtataataa
     8281 aaatcagaaa cttcaattgc taatgaatct acataatcat tatcatttcc atacttagga
     8341 acatccaacg caagttttct taattcttca taaccttccc aatcattatt tagtgctttt
     8401 gttaattctt ctaacgtagt caccttatcc tcaaatacta atttttttat agctgccaat
     8461 gaatttgaaa ctacacctaa ccctatacca gttaatacag gacctatatt atattttgct
     8521 cctcctttac ttaaatcaac tcccttatcc aaacatccat ctacacaagt agataaaaat
     8581 ggtcttggta ccatttcttt atgaatttgt tgagcaacag tagttcctat aacagaactt
     8641 tttacaagat atgctaattg ctttaaaaac tcatccttaa gttcttcaaa agaagtaaat
     8701 tctgtgatat tttttgtatc taaccccatt ttttctccag ttaatctact cttaccttca
     8761 ttcatagcat attctaaagc tgctgcaaaa ttgatattta cggctgaagt ccactgtcct
     8821 gtttttctaa aatgaggaac tacacaacca cagttactcc aatctctagc atcttctgca
     8881 tcatatccat cttgtaataa catttgtgcc cctgcactat cgctgtgtat agcagggaaa
     8941 ccagttcctt gttttaccaa tttagcaact gccattacga aattgtctgg tgctccttga
     9001 ctcactctaa cacttaatcc tggttggtga gtttttacac tttctgtagc ctttaaacac
     9061 atatatgaga tatcattcgt tccatcagta ccatttctct ttttaccacc aacagtaaga
     9121 ttttggaatt gattatatcc tgcaaagtaa tttgctgtat ttgctgatat agtccatacc
     9181 cattctgata acttaatcca aagtgcttct atcaattctt gagcttctga ttcagttatt
     9241 ttaccttctc tagcatcatt ttcataatat ggatacatat attgatcaaa tctacctaaa
     9301 tttaatgcca atggattttc tgatagaata ccacctaatt gaacaaacca tacaaattgt
     9361 attgcttcat aaaaattagt tggagggttt gctggtacat tcatacatat ctctgaaatt
     9421 tttataagtt ctaactttct cttttcatct gtttcttgct tactcatctc ttttgcaaga
     9481 tttgaatatc tttgtgccaa tgtcataata ccctgtgcaa ctatagaaac agatttgtaa
     9541 aaatctatct tttcaatatt ttctgaaact gatatatcta attctttaat ttttttatct
     9601 gcatcttcct taatcccttt atatcctttt acaaacaata tatcttgata atctggtgtt
     9661 acttcaccaa ctgcctgtct ccacttagaa tcattatcta ttattcctgt atctactaat
     9721 atcttagctg tatcttctgg gattcttgct aagaaaactt cttccaaaga ttttcccttc
     9781 caatatggaa atatttcttt tcttataaat tctatttgtt cttttgatat tacatatggg
     9841 tcttgagttc ttttatcaaa agtatccatt tctttgtcta cccattgcca agaaaactct
     9901 ggtgtcaata tccctgttct tctaaatttt cctgctgtac ctactattaa ttcattttca
     9961 aagattttta taggaagttc tttacatgtc tccaaaaaag ctttagctct tcttatacag
    10021 atagcttctc cctcagtttt tttatgtgat tctgtaaaaa ttctagccct ttcacaacat
    10081 atcataggtt tagaattaat atattcttgt ctaaatgctt ctattctatt agtttgactc
    10141 atttaaaatc cccctttgga taatatatac tacatttttg tatgttataa catactttaa
    10201 ttatattata ctgtccttta gcataaaatg gaagcaaaat aacttatttt ttttcatcta
    10261 ccttgaacac ctatatatta atactttttt tgttaaattt ataaaatcta aaaaaatttt
    10321 ctatgatata ttttctatat tgttatagtt atgtctaata tactaatgat ttgaaatatc
    10381 aaatttgtat actagactta gttttaaaac cttagttgat tgtttaactt tgttaacttc
    10441 aacatgtata tccttcatat aaggaagctc tacaacacct actcccttat taaagtcttc
    10501 ttcaaccata gaaaaaattt ctttctcagt taagttcgta tcagtcaatg atttaaataa
    10561 attgtatata aatttaacat atttatcact ctcagataac ttatcatctt ctggatactc
    10621 tttagcacag agaatatcta ttgttacttt tccttcttta tcgtcttcag acatagaata
    10681 gaataaactt cgaatatttt tttcaggtgg tttttcagca ccatttatag atactacaaa
    10741 gctaccatct gattttgagt tagtaatact ataaccactg tctattatag ctttcgaaaa
    10801 tctttgagat aaatcttttt ttacttcttc tctacctttc gcatcattaa tcttttgtga
    10861 gtaattatta aatagctcaa tagattcatc ttttttatct tcaacttgtt ctttcaatga
    10921 atctttacta atttgttctg agctacttaa gttcttatca gatacaggag acatacaccc
    10981 taccatcaat gctaagctaa gatttaaagc cacaattatt gccactctat ttatattcct
    11041 ttttactttc atacacaatc ctcccatcac actaaccgct ctaaagtatt attcattgca
    11101 ctagaatata tttatatttt ctttatatta aagatttatt attcaataca tatcaccttc
    11161 ctatccaatt cttataactt actattagta aaattataag ctccttaata cagaaataag
    11221 gatgttatct tttgttatta aaagataaca tccttatttt tttgtatcaa tacatatgtc
    11281 ccaactggta acttaatcgc cccaactgtc agtatcttgg tccaatggta tatatatatt
    11341 tagtataaat ttatcgatac tattttcaaa taataattct ccattatatt tttgtaaaga
    11401 agatttaata ctctgaatac ctattccatg tacgaactta tccattttgt ttgttaacaa
    11461 tatattttta ttaaaagtaa gctgatttag tttactattt tcacatttaa gtacaaaaaa
    11521 tgaccttgtt atagttcctt taattctaat atatttatta atattttcat ccactatttt
    11581 atcacatgct tctattgcat tatccaaaat attagaaaat atgcttgaca catctatagg
    11641 ttttacaaaa tttacttttg aaaaatttat agagcaagta aatttaattc ctttttttaa
    11701 acatacttca ctcttttcgt tgattataat gtctaatatc atatttcctg tattatagat
    11761 atatttaaat gtttttattt catcttttaa attgttaaca tattcattaa tttctttgct
    11821 attgtttcta aggttgtcta tacaataaat gtgattattt atatcgtgat atagtttctt
    11881 aactcgcata tgagattctt gaatacttaa ataatgcgca tattgcatat ctaacttatt
    11941 ttttattaat tcagcttgag attttaatct attctctttt attatttctt taaacaatag
    12001 tataagaaat atactactag ctcctaccaa ttttggatta acaaaattat caaaatagta
    12061 tccttctgaa tgtaatgacc caaaccgttc taattttttt aatacataat ttacaagcat
    12121 attcattgta ttaac
//
LOCUS       DAYATN010000117        12021 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.117, whole genome shotgun sequence.
ACCESSION   DAYATN010000117 DAYATN010000000
VERSION     DAYATN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.117"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(284..2014)
                     /gene="lpdA"
                     /locus_tag="ACGI8A_002885"
     CDS             complement(284..2014)
                     /gene="lpdA"
                     /locus_tag="ACGI8A_002885"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFH0180875.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(2181..3227)
                     /locus_tag="ACGI8A_002886"
     CDS             complement(2181..3227)
                     /locus_tag="ACGI8A_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFH0180876.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            complement(3240..4226)
                     /locus_tag="ACGI8A_002887"
     CDS             complement(3240..4226)
                     /locus_tag="ACGI8A_002887"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFH0180877.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            complement(4251..5213)
                     /locus_tag="ACGI8A_002888"
     CDS             complement(4251..5213)
                     /locus_tag="ACGI8A_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HFH0180878.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            complement(5251..6204)
                     /locus_tag="ACGI8A_002889"
     CDS             complement(5251..6204)
                     /locus_tag="ACGI8A_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFH0180879.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            complement(6649..8022)
                     /locus_tag="ACGI8A_002890"
     CDS             complement(6649..8022)
                     /locus_tag="ACGI8A_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0180880.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            complement(8119..9765)
                     /locus_tag="ACGI8A_002891"
     CDS             complement(8119..9765)
                     /locus_tag="ACGI8A_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFH0180881.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYVQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            complement(9787..10953)
                     /locus_tag="ACGI8A_002892"
     CDS             complement(9787..10953)
                     /locus_tag="ACGI8A_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HFH0180882.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFAMIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLVKCVAQEWYPIEY"
     gene            11170..12021
                     /locus_tag="ACGI8A_002893"
     CDS             11170..12021
                     /locus_tag="ACGI8A_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0180883.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKNLPKI"
BASE COUNT         3685 a         2323 c         1283 g         4730 t
ORIGIN      
        1 tttttatatt ttacaaataa tacttttttt aaaaacaaaa aaatcacttt tcctaattac
       61 aattgaaatc attctaaaat gataatgtta ttttatatta ctatcttttt tgtacaacaa
      121 aaactacaag gattctatat tctatatttt tccataaaaa atacccctta ttatgcaaat
      181 aaattatttg cataataagg ggtatggcta agtaagtgtt ttaaaatttt atgatataaa
      241 ttaatattaa taatagactt aaattaataa tttatgttta gatttatttt ttaggtaagt
      301 gtaaacattc tccatttaca tctgccaaag cttccattaa agcttctgaa gtacatggat
      361 gaccaaatat caactcactt gcttcatcag taggtatctc taatgcttta aatgatgctg
      421 catgatttat aagttctgct acaccacatc caaacatatg tatacctaaa atttctccat
      481 atttagcatc tgctactacc ttcacatatc cttgttcttg accagaagct aaagctcttc
      541 catttccagc aaaattaaac ttaccaactt ttacattata tttttttcta gcatcctctt
      601 ctgtaatacc aactgatgct acttctggaa ttgtataaac acaactaggt aatgcaccta
      661 agtcaacttc tttattcaca cctagagcat ttgaagctgc tacctctccc attttaaaag
      721 cagcatgagc taacattaca cctccagtaa catcacctac tgcatatatg cttggtatac
      781 ttgtttccat tttactattt acaactattg aacctttatc tattttaata tctaagtcct
      841 ctatgccaga aagattagct tctcttccta tagcatatag acataaatct gcttttattg
      901 gctcttcacc ttcaatgcac actaatatgt tatttccttc ttctttaaat tcagaaactt
      961 tctttttagt taatacattt ataccttttt tactaagaga atattttaaa gattcgctta
     1021 actctttatc cattcttggg attactctat cttccatttc tactatagtt actttagaac
     1081 ctcttgaatt gaatatctct gcaaattcac aaccaatcac tccaccacca attattacta
     1141 attcttctgg cactgtttcc aaatctaatg cttctgtact agttattatt aaatttgatt
     1201 ctattccttt tataggtaat atccttactt tggaacctgt agcaattatt atattttctg
     1261 tatctaaaac ttttccatct gataaaatta ctttatgctc ttctttaaca ctagctttaa
     1321 gattaaacac atctacatct ctacttttta ataatccgcc aacaccagct gtaagttttt
     1381 taactactct atttttatat tttatggcct tcttaatgtc ctgttcttta tctacagtta
     1441 cttttacacc tctctttgat aattggtcaa tttcttctaa tatttctgct gtttttatat
     1501 aagtctttgt agggatacaa cctctattta agcaagtacc tcctagtata ttttcttcaa
     1561 ctaaagcaac tcttccacct aaaagagctg cttttaatgc tgataaatat cccccaggtc
     1621 ctcctcctat taccactaca tcataatcat gattacactc ttttttgttt attttttctt
     1681 ctttaaattc tatattttga ttctcttcaa ctatatcttc ttttgaaaca tttccagata
     1741 aatatttagc ttttacttcc tcttgatttt cccctttttc tgctattaca cctattactg
     1801 taaatactgg taatacttca ccttcctctt tatgtatgat tacagcaagt gtaccttctc
     1861 cttctgactc tatctccata ttaactttat ctgttgttat ttctagtata ggttctccaa
     1921 tctttacttc ttcgccttct tgctttagcc aagatactat agtaccttct tccatggcta
     1981 ctcctgcttt tggcattatt acttctactg acataggctc tcctcctatt tattttctta
     2041 tattttaatt aagcaaatca tattcacata tagttactgt taaaaataat tcttaatata
     2101 taaaagcttt aaatttactg aaacaattat aataatataa tctattcaaa ctatttatat
     2161 cagtaacaaa atatcaaaac ctatacaagc attgataaag gattttctaa caattctttt
     2221 aaatctttta agaattttgc agctactgtt ccatctatta ctctatggtc tgatgttaat
     2281 gataagttca ttataggttt tattttagcc tctccattta ctggaacaaa cttatcttgt
     2341 gtagcaccta cccctaagat tgcacttgaa ggcatattta ttataggtgt aaatgtagtt
     2401 ataccataca ttccaacatt acttattgta aatgtattac cttcttggtc tgctggcata
     2461 agtttccctg tctttacttt ttccgcaagt tctttgcttt cttttgctat ttcttttaaa
     2521 gactttttgt ttgcattttt tactaccgga acatataagc cttcatctaa accaactgca
     2581 atagctatat ttacatcttt atatctaaat ataccctcat ctgtccatgc tgaatttaag
     2641 gcttgatgat ttggaagtat tttagatact gccattacta ttaaatctgt catagttaat
     2701 tttactccag ttgattcttt tactgtgtcg attaactttg ctctaagtac cttcaattct
     2761 gtagcatcta cttctatatt gaatgtaaat acaggagcac taaaataact ttctgacatt
     2821 cgttttgcta cagtagctct cattggattt ggcttttcaa atattccttc tactgtgtta
     2881 aacatgcttg aattttcatt ttcaattttc agctcttttt tctcactaga tttagatgta
     2941 ttagtcgata caatagtttc ttttgcagtt aatttttcca catcacactt tcttatcttt
     3001 ccattatatc cagtacctac aatagtttct aaatctatat ttaaatcttc tgctattctt
     3061 ctagctaaag gagtagtatt tgctttatta tcttttaaat aatttaacac atctactaaa
     3121 tgaatccttc cattttctcc acttccaatc agcctatcta attttatatt gtcttttctt
     3181 gcctgacttc tagctgctgg tgtggctttt attttatttg ccaccatatc tatccctcct
     3241 tatctaacaa ttaaagattt tattgcttct tctatttcat caactcttgg gacaactgct
     3301 ttttctaact ctggattata tggtattggc acatcttttc cacaaattct ttttactgga
     3361 gcatctaaat aatcaaatga ttcactttct gttatcagtg ctgatatttc tccacctagc
     3421 ccaccagtct tagctgcttc atggcaaatt aaaactctcc cagttttaca tacactcttt
     3481 actatagtct ctttatctaa tggatataaa gttcttaaat ctataatttc aacatttata
     3541 ttttcttttg atagagtctc tgctgcttct tcaacacttt gtaacattct tccataagta
     3601 attactgtaa catcagtacc ctctctttta atatctgcct ttccaatttc aattacatag
     3661 tcatcttctg gcacaaaacc tttttttctg tataacaatt tattctcaac aaatatgact
     3721 ggattattat ctcttatagc tgctttcaac aatcccttag catctgctgg tgtagaaggt
     3781 gcaactactt ttactcctgg tacatgacaa aaccaagctt ctaaactttg tgaatgttgc
     3841 tctgctgaac cagttccaga acctcctggg catctaacaa ccattggaac ttgtgctttt
     3901 cctccaaaca tatatctcat ttttgccgct tgatttacaa ttgcatccat agaaattgtt
     3961 acaaagtcca taaacattac ttccataata ggtcttaagc cagttgctgc tgcacctgct
     4021 gccgctcctg ctatagctgc ttctgatata ggagtatccc ttactctttc tggtccaaat
     4081 tcatctatca taccaactga tacaccaaat gctcctccat atattcctat atcttctccc
     4141 ataaatatta cattttcatc tcttctcatt tcttctgaca ttgcttcctt tattgcttgt
     4201 gcatatgtta attcccttgt actcattttt attttcctcc tttaccattc ttagtctgca
     4261 tatacatcct ctaataaaga ctctattttt ggatttgggc tattttgagc aaactctact
     4321 gcatcttcta ttgattgctt tgcaaattct tgaatcttat ctaattcatc ttcattagat
     4381 agattatttt ctataagata attttttaaa aattcaatag ggtcttttgc tttccatgat
     4441 tctatttctt cttttgttct atatacattt gcatctgatt ttgaatgacc taaccatcta
     4501 taagttctac tttcaattaa tacagggcct tctcctcttc tacacttctc agcagctttt
     4561 tgtactgttt cgtatacttc tattgggtta tatccatcta ttgatattcc ttctattcca
     4621 taagaagctg ctctagtggc aatactttct atattcatat gacgttttat gcttgttgac
     4681 ataccgtata aattattctc acagtaaaat attattggca atttccatat tgaagataaa
     4741 tttattcctt catgaaaagt accttcattt gatgctccat caccaaaact acataaaact
     4801 attttgcctg tttttttata ctgttgtgta agtgcagctc ctggagctat tgtaagacct
     4861 ccacctacaa ctccatttgc tcctaagtta cctgactcaa tatctgctat atgcattgag
     4921 cctccttttc ccttacaaaa tccagtttct ttacccataa gttcagccat cattttattt
     4981 aggtctattc ccattcctat aaattgacta tgtcctctat gagttaatga tactaaatct
     5041 cctttttcta aagccattac agccgccaca cttgacgctt cttgacctac agataagtga
     5101 gttgttccat gaaccatacc tcttgcaaaa aaccaactaa ctttctcttc aaattttcta
     5161 gcctgattca ttctcttata catttcaagc aatgtttctt ttgatattga catatacatt
     5221 ccctcctgtt aacttttatc tatctttcta ttagtcacaa tcttctaata atttaagtgc
     5281 taaactgtga ttaattgcta aactatttat aaatttcgct tttaatgatg cctttattgc
     5341 ctctattttt tcatctcctg aagcgacacc tattgttctt tttatctttt ttaaatcttc
     5401 cagtttcact cctaatactc tttgattaaa ttcaagtata gaagtttttc cttctttatc
     5461 aaaggcttgt aaacaaattg cacctattga gttgtatttt tttaaatctt ctatatctgc
     5521 ttcattcatg tatccagaag ccattattgt tgagtttaaa ctcataggat ttcctatacc
     5581 cacaattgct atagttgttt tatctattaa atctagtact tctttaattt tttcgtcttt
     5641 gcaaagtttc tcttttgtag acaagtcaga cataacagct ggagcatgta ataatttaaa
     5701 atcacctcca aaagccctcg ctaaacttac ggctatttga tttgcatgaa tatctatttc
     5761 attgtctcca atccctccta atagaggtat aaatgtaaca tttttacagt tacttttttc
     5821 cacatatctt ggtatttctt ttagtgttgt tcctattgaa acacctatta tatcattttc
     5881 ctttactact ctagttaaat attcagatac agctcttgct agttcttgtt tttgtgttag
     5941 tgcatcctgt ttatcatcta ctactattac ttcttttaat ttatattttc tctccaaact
     6001 tctctctatt ttttggtaat catttttcaa aacatcaact atctgtattt ttactattcc
     6061 ttgttcaaaa gcttctttta atattcttga tattgttggt cttgatattc ccaattgatt
     6121 tgctatttcc tgttgattca aattatcttc gtaataaagt gaacaacact tcatcattaa
     6181 tcttaagtct ccaatttttt tcatttgctt tcctccaaaa tcaagctttt caattatctt
     6241 tacatttgtt caatcatttt acttaatttc agtaacatta tagaaggggt tcatataaaa
     6301 agtcaactgt ttttttgtat aacaaaaaaa aggtgatact tttcttgtac caccaccttc
     6361 ataaatagtt gaaaaattgc ttcttagtgc atattatata ttacttctaa ttgttaaata
     6421 tcatctccat aaattaaaaa ttaattgtcc acttataatg ctttaaataa caataactta
     6481 ttaaatcact tctaaaaaac caaccatttc atcatttaaa gtcttcttat atttttatta
     6541 ttcgcttctc taaaaattct tcaataatca taaatatata atagttttta tagggaacat
     6601 ttacaataga ccttaattaa acttaagact ttataagaaa ctttttcatt atttagtatt
     6661 taattcatta agtatagtac tatattcttt atcaagctta taaaaagaca ttatgataat
     6721 ttgtaatatt gttataaata taggtaggta tataaaacat acttttattg atactatagc
     6781 taaatcactt tgagtagctg atgttcctac ataaccacca attgataata ctgcacctaa
     6841 tattgctgaa cctaatccat ttcccacttt aaacccaaaa ctagatgcac tatttataag
     6901 cccctcagtt ctatatccag tcttccattc tccatattca atagtatctg aaacaatagc
     6961 aaacattgtt gatgctattc cagcacttcc tattcccttt atagcacttg caaatattac
     7021 tcctatatga ttttgtccaa ctattataaa cataacattt gacgatatta atattattga
     7081 acctactatt aatacatttc tttttccaaa tttttttata aaaggagcaa ccataaacat
     7141 agctataatt tgggatatat ttgaaaccat atttatagga ccaaataatt tttcatctcc
     7201 caaaataaac tttgcataat atacggatga accaccattt atcgcaaata caataaatat
     7261 gcaaactaaa cataatgtta tttgtatcca gtacttattt ctaaataaag atttaatacc
     7321 tattttaaat ggtattactt cattcctatc ttgcttaact gcttttactc tttccttggt
     7381 tccagtaaat gttataaaaa ataccattat agatgctatt ccaaatacaa agaatgtttt
     7441 agtccatgct gataaattat ttccaaagta ttcaactaga ggtagtgtaa gtccatttat
     7501 tagtaaggta ccacaagtgg ctaatatcat tctaaatata cttaatactc ctctttcata
     7561 ctggttttga gttataagtg aatttagagt tgcatagggt acattgattg ctgtataaat
     7621 aacagtagaa actaagttat atgtaataaa aatataaatt aatttagaag tgataccaag
     7681 atttgatgga acagaaaata ataatactgc tgctatagca aaaggaatcg ccattctcaa
     7741 aatccaaggc ctagccttac cgtatttgga tttagttcta tccactataa ttcccattgt
     7801 taagtcacta aacccatcca gtatcctaga tacaagcata atactaccta cagctgctgc
     7861 actaacatct gcataattag tataataaaa cagcaaaaat gctgacatag ctgagtatat
     7921 gatattgcac cccaaatccc cacaaccata agaaattctt tcaaaaaatg aaatcttttc
     7981 atatctaatc gtagttgctt taacattaac ttctgtattc ataataccat actccttttt
     8041 gtaagatttg ccttctaatg tttcttattt cagagcataa gatttacctt tgctctgaaa
     8101 attttagtaa tgattttatt aatcgacatc ttttgattca tctactatat attcaaaaaa
     8161 atcaaaatca gcatagtgtt tatgctttac acgatctgca caagttaatc ctataaacgt
     8221 acctgtaaat tctccatatt tacagtactc atctgaaaac ttagttgtat caaatacctt
     8281 tcctattctc tggtagtttt tttcatcata actccattca aaatatgact tacgaccttg
     8341 tacatacaga cgaagatata ttggtatatc ttttattggt atcctagtat ttaaaaattc
     8401 cgttttctct ccattttcaa gatgtattat tgatagtgca ctttgaccca atgtttcact
     8461 ataatacttt cttaaattta tataattcat attatcataa tacattatca aacccgcact
     8521 atgctgatgt acctctgggt aaaactccat cttagtggtt attcttgcat atactgaagt
     8581 taattttctt gccaatatgc tcactttatt taatgatgtt cttgattctt gtcctctaat
     8641 ccttacatat cctggtctag acttaacatc tgcaaatgat aatggtgaaa tcctaggagc
     8701 atagtactgt aaacctaatt catttgaatc aaagtcatca aaacttggta tagatgaaac
     8761 taaatgttct tctagtttac tttctgatac gtattctttt gccaaattac tttcatcttc
     8821 cattcgaagc caattatcct ttgtccattt cattttttga atagctgttt ctctacctaa
     8881 agtacacctt aactctggta caaatggtct agatgttaga tgaactaaat atacttcacc
     8941 tgatgttgtt tctatatagc ttccatgacc agatttttga agttttgatt ctggattata
     9001 gtatttaggc tttagatggt cagggtcatg tctttcataa aaatcgcctg gaattgaagt
     9061 tacaattgga ttcattgaat ctttttcata aggtccccat atatttttag cccttcccat
     9121 agttacacta tgaccatatc cagttccacc ttctgcacac attatataat agtaatctcc
     9181 acgctttgtt atatgtggtg cttcaataca acctctatca gttccccctg accatattct
     9241 ctttggatat cctactattt ctttcttttt tgtacagtat tccactaaac atataacacc
     9301 aggtttttca tatccttctc ttgtctccca gtctaatgat gcaatccact ttttaccatc
     9361 atcatcatga aaaatagacg catcaaaacc tgatgagtgt agatatactg gttcactcca
     9421 ctctcccttt atatcttttg atgtaattaa ataattatca acatcaaaat atcttgcatt
     9481 catagagttc ataattccat aaactatata aaataaatct tcttcttcac aataagtcaa
     9541 acagggtgcc caaattcctt tagatgaagg aagcttcttc aaatctattt ttgtttcatc
     9601 tgtcaaaata tgagtatata actcccaatt ttttaagtct ttagagtgat aaacaggtat
     9661 acctggaaac cattcaaaag atgaaactac aagataataa tcatctccct ttctacaaat
     9721 acaaggatct ggattaaaac ctggaagtat agggttttta atcatatctt ttcctccaat
     9781 tttagtttaa tattctatag gataccattc ttgagctaca catttgacta ggtcccaact
     9841 atcccatcct atccaacctt cttcattaac tgtatctagt aataacttat aattccaata
     9901 gtagtgacca cttccatttc tccatgcatc aagctgtgct tttgctatct tcctatataa
     9961 ttctctttta tcgtcttttg ataatttatc tatatttgat tcaatcccat ttagaggtga
    10021 ttgacctcca tttgtatcta taccacaagc atatgaatta aataagctcc actccccaca
    10081 tataacagga aaatattttt gcatttgtaa aatatcttta gcataatttt ccctaatata
    10141 tttgagatag ctatcaatac tttgttcaca tccatctgct tcagctaaca tcaaatattg
    10201 gtgtgtatct agaacaacat ttttaaattc ttcctctctc ataaagtctt tccatgcttt
    10261 taattcaaat gcatcatgaa atactataac gttttcctcc ctcatataag gacgcattac
    10321 cttatatgct tttatataaa aatcacgtaa aaactctaat gaattaggtt tactccccct
    10381 tgccatctcc ttatctacag ccttaaaccg atttggaaca ttcattatgt tccacattct
    10441 ttctgttata ggctcattta aaatttggat tccatacaat tcatgcctca tcccataacg
    10501 ttttgcaagt ctttctaata catttaatgt aaacgctaca tattctggat tttgtgacca
    10561 gctacatata cctgatattc ctccattatc aaatccattt tggcttcctg gaactgtatg
    10621 taagtctatc aatatactaa gcccatactt atctgcccaa gcaaatgctt tatcaagttc
    10681 tttaacacat cctatgaatg gttcacaatc tccaaaaata aagtatggta ctggtatacg
    10741 aactgaatta aatcccatag atttaatcat tgcaaaatct ctctctgtga tgtattcact
    10801 acgatgagtc ttaattctag attcatatgc ctctcttgaa agctgtcttg gtaaatagta
    10861 ttcatcttca gcttctgtgc cttcaaataa acttgaggtc atccattttt ctaaaactaa
    10921 ccaacctcct aaatttacac cttttatata cacataaaat cctcccctct tatcttattt
    10981 aaataggtat attttgcact tataataaaa taattttttt aagttatttt aatatacagt
    11041 tattatattt gattttatcc gttttcttaa gtatgtttat tatctaattt attaatctat
    11101 attatctttt taatattgaa cattataaat ttactattta taatttaaaa ttataaggag
    11161 gatattatta tgaacagaaa taatttttca catgaaataa ttgaacctat tgataattta
    11221 gatgttaatt tcaaactatt tgatgatagt ggtagttatg tagcaaatca ttggcataac
    11281 tcattagaaa taatttatat aacttctggt gatttacaaa ttaacatgga gggttacact
    11341 tataatttaa aagctaatga gtgtatgttc attaattctg gaattattca ctcaactaga
    11401 tgtatttatc aaaatacctc tatattattg caagtaccat tgtcttttct aaacaaatat
    11461 atccctgatt ttaaaaattg ctattttgac tttaaggtta atgccaatga caataactat
    11521 aaaaaaaatt caagtaaagt taaacttata ttagaaaaca tgagagaaat taaacttagt
    11581 tctcctcctg cagctaatct gctctttaca agcttattat ttgaactttt atttgaatta
    11641 tatactaact ttaaaatatc tgtaggtaat aaaaatatga aaaaaacagt acttgatttg
    11701 tcaaaatttg aaccagtttt agaatataca aagattaatt acaaaatgcc tatttctatt
    11761 aacaaaatcg ctaaggtagc acacttacaa ccagaatatt tttgtagaaa atttaaacag
    11821 tatatgggac aaacttactt agagtattta aatgatgtgc gtatttctca tatttataaa
    11881 gacttaatta atacaaatga taccttatgc agtatattag aagctcatgg atttactaat
    11941 acaaaattat tttacagaat ttttaaggaa aaatttaaat gcactcctaa acatataaga
    12001 aaaaatctac ctaaaatatg a
//
LOCUS       DAYATN010000118        11776 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.118, whole genome shotgun sequence.
ACCESSION   DAYATN010000118 DAYATN010000000
VERSION     DAYATN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.118"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            160..789
                     /locus_tag="ACGI8A_002894"
     CDS             160..789
                     /locus_tag="ACGI8A_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180884.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            790..1572
                     /locus_tag="ACGI8A_002895"
     CDS             790..1572
                     /locus_tag="ACGI8A_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180885.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1565..2443
                     /locus_tag="ACGI8A_002896"
     CDS             1565..2443
                     /locus_tag="ACGI8A_002896"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180886.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2476..3435
                     /locus_tag="ACGI8A_002897"
     CDS             2476..3435
                     /locus_tag="ACGI8A_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0180887.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3750..4442
                     /locus_tag="ACGI8A_002898"
     CDS             3750..4442
                     /locus_tag="ACGI8A_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0180888.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4683..5456
                     /locus_tag="ACGI8A_002899"
     CDS             4683..5456
                     /locus_tag="ACGI8A_002899"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0180889.1"
                     /translation="MYLGSSFLGRNISVILKQQHKLYQSMIQEHKKIHSEHLTFINQW
                     IHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLDMAMHFARADNFQKDFIVEK
                     AKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDVKWMKFIIGQLITNGVKYSK
                     DYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDPFFTGENGRKFGESTGMGLY
                     LVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV"
     gene            5724..6575
                     /locus_tag="ACGI8A_002900"
     CDS             5724..6575
                     /locus_tag="ACGI8A_002900"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFH0180890.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6679..7425
                     /locus_tag="ACGI8A_002901"
     CDS             6679..7425
                     /locus_tag="ACGI8A_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180891.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALLRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7425..9341
                     /locus_tag="ACGI8A_002902"
     CDS             7425..9341
                     /locus_tag="ACGI8A_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0180892.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVIMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKSISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFFIVR
                     NNYLKEIRLNLVNF"
     gene            9621..9854
                     /locus_tag="ACGI8A_002903"
     CDS             9621..9854
                     /locus_tag="ACGI8A_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFH0180893.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
     gene            10050..10478
                     /locus_tag="ACGI8A_002904"
     CDS             10050..10478
                     /locus_tag="ACGI8A_002904"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFH0180894.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKNFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(10932..11702)
                     /locus_tag="ACGI8A_002905"
     CDS             complement(10932..11702)
                     /locus_tag="ACGI8A_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180895.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTFYCCGNCDSEISLSQREHFKI"
BASE COUNT         4632 a         1130 c         1947 g         4067 t
ORIGIN      
        1 aaataaatta catatactat ttaatatttc atttaaaata ttaaaaaatg tagataaaaa
       61 ttgttttatt aagaaaaaat taatatattg acaaaattta agtgctcatt taaactatta
      121 attgataata atattcaata ttaaataata ggaggcgtta tgaataaata tcttctaata
      181 aatccagtgg cagaaagaat gtatggagaa aagtttcacc ttattaaagc aaatttgata
      241 aaaaaggggt acataatagc agaatgtgaa ccacagttag aatatgtaaa aaagcagtac
      301 aaagagtata caaaaaagac tgaaaataca atgttggatt gtagatgtcc agaaagtata
      361 agtcttttaa aaagaaataa tttgataaat gattttgaag tacctatgat agaaccaata
      421 ttagtaagga cttgtagggt tttatacgat aaatatataa aaagtaaaga tgatatgttg
      481 gtagtaacct gtccatgtac acaattaaga gactttgcaa gtgaaaagtt taaggataag
      541 ggtaatgcta tgttttatac atggaaagag tttgctgaac aagaaggtgt aaaatcactt
      601 ggaaaaatag atgaatcacc aattccatta ggatatttca aagatacttt tgaaaaagtt
      661 cttgaagtat caagtgaaga cgaaatatta agtgaaatac aaaaaataag aaatggcttt
      721 gaagataagt atgacatagt agaaatgttt tattgtaaag atggttgtaa taatggagat
      781 ggtttgtaaa tggtcaataa atttaataat aagtttttaa ggctagtaaa gagtgttttg
      841 atatttttta taattctttt attatggaaa attacaaatt atcttggtat ttggagtgat
      901 tatatattac catcacctga aaaggtatat agtacatttt taaatatgat aagtgatggt
      961 tcaatattta ttaatgtata tgcaagtatg aaaagagttt tgataggttt tgcaataagt
     1021 actgctatag gtgtgccttt agggatattc tttggaattt atagtggagt atacgaatac
     1081 tttaaatctc taataaattt tttaaggaat acacctcccc ttgctttaat accaatgctt
     1141 atattatggt ttggtatagg agaggaatca aaaattataa ttatagtttt agcatccttt
     1201 ttcccaatct ttacaagcac attgaaaggg attaaaaatt gtgactcaaa actgattgaa
     1261 gtgggtagag tatttgagtt ttcaaagctt cagataatat ttaaaataat aataccaaat
     1321 gccattcttg atattgctgt tgggttaaag ttagctcttg gatatagctt tagagccatt
     1381 attggggcag aacttgtagc agcttcatct ggtcttggat acttgatatc tgatggaaaa
     1441 gaaatgtcta gaacagatgt tgttattgta ggtataatcg taattggact tttaggaata
     1501 attacagatt acatattttc gattattgtg aagaaggtaa gtaagggaaa gatggtggaa
     1561 gcatatgaat aatttagtat gtgactctgg atatataatt agaaatttat ataaaagtta
     1621 taaagttgac aatgaagaac atgtagttct tagtgatatc tctttaaaca tagatagaaa
     1681 ccatataaca gtaattttag gtgaaagtgg ttgtggaaag actaccttgc ttagagcaat
     1741 tgcatcactt gaaggtataa atagtggtga aattaggttt attaatgata ataaagagta
     1801 tagacctaat gttggttttg tttttcagga gagcagatta atgccttggt taaacgtaag
     1861 tgaaaatata gcttttcata atcaaagaaa aaatactatt ttaaacaagg tgcttagaaa
     1921 atttaaacac aataaaagaa ttagcagtaa aattataaat gataatttta gaagcttaga
     1981 aacaaatgat aatttgaaca aatcaaataa atataatagg aaaagtaata ttgatgtaga
     2041 aaaatatctt aagatgatga acttgcagaa atttaaaaac tcttatccaa atgaattgtc
     2101 aggaggtatg gcgcagagag taagcatagc tagagctctt tctttcaatc cagatatgtt
     2161 gcttatggac gagccttttt cggcattaga ttattttaca aggattgata tgcaaaatga
     2221 agttataaga atacataagt caactaataa aggcgtgata tttgtaactc atgatattga
     2281 tgaagcgtta aagataggca agaaaatagt tgttttcaca gacgaaagaa aagtaaaaga
     2341 atttaatata gaagatggtt ataatagaga tttaacttca gattactata ttaaattaaa
     2401 aaaagatatt ttaatgacaa tgaaatcaaa taaaggcatt taaagaaatt aataatattt
     2461 taaggagtaa agtaaatgaa attaaaaaag ctattgatag gtataacaat attaacactt
     2521 gcaacagcat ctttagctgg atgtgctaaa aaaaataatg gagaaaaact ttcagaaata
     2581 aatttaactt atgtaaaatc accattaaat gttccttcta taattcaaaa acaggatgat
     2641 ttatttggaa aggaatttaa aaaggataat ataaaagtta atttccacga aattactaca
     2701 ggtccagagc aaacacaagc tttagctgct ggagaaatag acttcttaca tgcactaggt
     2761 ggtacatcag ctttaatagc tgcatctaat ggagtagagt taaagatatt aaacacttat
     2821 agcaggtcac caaaaggatt tatgattttg actaataata attcaataaa atcagcagca
     2881 gatttagtag gtaagaaggt ggcaggccca aaagggacta tattacatca ggtattgatt
     2941 tcagcacttg ataaagaagg attaagtatg gatgatgtgg aatttgttaa tatgggtatt
     3001 ccagaggcat cagcagctct ttctgatggc agcgtagatg ctgcattgat tgctggtcca
     3061 gcagctctta aagctatgaa gtcaggtagt aaacttgtag caaatggtga aggtttagta
     3121 gatggtataa tagtaactgc tgttagcaca gactttgcag agaaacatcc agaaatagtt
     3181 gaaagattta tgaaggttga aaaggaaact ttagaatatg taaataataa ttttgatgaa
     3241 gctatggaaa aagtagctaa agaagttgat ttaagtcttg aagaaactaa agaattatat
     3301 gcttggtatg attttagttt agatataaca gataaagaca tatcttcgct tgaagataca
     3361 caagacttct taataaaaaa taagttacaa gagaaaaaag tgaacataaa agaattaatc
     3421 tataatgcta agtagaaact atgataaaat aatactaagt aagaaatata gtaaaagttt
     3481 aagttacctt attaaggtaa ctttttctat tttgaattag taatttcaat ttattttaga
     3541 atgattagtt gataataatg attaattaat cataaaaaga tactttttta tagagtttta
     3601 ttaagatgtc taataatatg gttatactta tgaagtcagt aattgtgaag ttagtaatat
     3661 gattaaattt atatattgta aaaagattat aatatattaa aattggactg ttaatatgat
     3721 gagtaaatag gtatattagg aggaattata tgagttataa agtatatatt gtggaagatg
     3781 atatatcaat aagtacatta cttaaagatt atattgagaa atatggattt gaagtgattg
     3841 ttgaagaaaa ctttgatgat ataatgatta catttgaaag ttttaagcca cacttagttc
     3901 tattagatgt aaatttacct aaatttgatg gtttctactg gtgtagatgt attagacaac
     3961 agtcgaattt acctataatt tttatatcag ctagagatgg taatgtagac caaattagag
     4021 ctttagaaag tggagcagat gattatataa caaaaccttt ttactatgat gtagtaatgg
     4081 caaaaataaa gagtcatata agaagggtat atggtgaata ttcacctaaa atagacgaaa
     4141 gaatagtgga gctagatgga ttaaagtttt atccagagag gttagaacta gaattaaatg
     4201 gtgagaatct aattataact aaaaaagaag gtatactgat tgaatgtctt atcaagagat
     4261 acccaaaagt agtaagtaga gattatcttt tagaaaagat atgggatgat attgagtttg
     4321 tagaggaaaa tactttaaat gtaaatgtta gcaggataag gaaaagattt gaggaattag
     4381 gtatagataa tgctgttcag actgtccgag ggttaggata taaattaaat aagaattggt
     4441 aaggagaaag tatgaagtta tttttaaaag attataaagg ttatattttt atatattata
     4501 taggcatagt tatgacaatt atttactgta atcttatgaa gtttattagt tttggagaga
     4561 gtttatatat tcttttattt agttctttta tattgttatg ttttttaata tataagtatt
     4621 ataaaaatag agaagtatat aatctgtttg aaaaaggagt acattctttg gaggagtcat
     4681 ttttgtattt aggaagctca ttcttgggga gaaatatatc agttatttta aaacaacaac
     4741 ataaattgta tcaatcaatg atacaagagc ataaaaagat acatagtgaa catttaactt
     4801 tcatcaacca atggattcat cagatgaaaa caccaatatc agttataagc cttcagcttc
     4861 aagattatga gggagaagaa gtaactgaaa atataagaaa agaaatagat agattaaata
     4921 aagggttaga tatggctatg cattttgcaa gggcagataa ttttcagaaa gactttatag
     4981 ttgaaaaagc caaattaaat gaaattgtat ctagtattgt aaatgaagaa aaaaggttat
     5041 ttataaaaag tggaatgatt cctaagattc aagtagacaa atttacttat gtttatacag
     5101 atgtaaaatg gatgaaattt ataataggtc aattaattac aaatggagtg aaatattcta
     5161 aagattatag taactaccta actataaaat ctgaagaaaa taataaatat gtaattttaa
     5221 gtataataga tgaaggaatt ggaattgttt ctaaagattt aaaaagggta tttgacccat
     5281 tttttacagg agaaaatggt cgtaagtttg gagaatctac aggtatgggt ctttatctag
     5341 taaaaaaggt atgcgatgat ttaggtcata aagtaagtat aaaatcagaa gtaggaaaag
     5401 gtacagaagt ttcaatctac tttaataaag atgtaaatag tttaataaag gtgtaaatag
     5461 tttgataaag atgtaaatta tctgttacaa tagggttttt agttaattgt acttttttat
     5521 tatacaattt attaaatgaa atattcatag ttaaataatc aaaatttaag agcgtttatt
     5581 tgcaaacctt acatagatgt aaggtttgtg taatgtttgc agatagttaa attttctaat
     5641 ctaaattaca attaatataa gaaataacat aagagaaata acataaaaaa agaaattttg
     5701 taaaaaagga aggatgttta ttgatgagtt tagatgttat ttttatattg aaatcagtca
     5761 taatagctat agtagagggg cttacagaat ttattccagt ttcatctaca ggtcatatga
     5821 ttttggttgg taatttaata gattttaagg ggcaatttgc tgaaatgttt gaggttgtaa
     5881 tacaacttgg ggctatatta gcagtagtag tactttattg gaaaaagata aaagatagcg
     5941 ttatagaatt ttttaaattt atatttactg gtggaaaaga aggaaagatt ggttttagat
     6001 ttggaatgaa tgtaattata ggttgtatac catttgcgat tataggagtt ctattttacg
     6061 ataatataaa atcattgttt aatttacaat ctgtaattat aggatttata gtgggtggga
     6121 ttttattatt agtagtagaa actttgttta gaaaaaagaa tcattccaca gataatatag
     6181 ataaaattac tccaatacaa gctttaaagg ttgggacatt acaagttttg tctgcatggc
     6241 caggtatgtc aaggagtgca tctactatta tgggtggatg gatagcagga cttaattctc
     6301 ctacagcagc tgagttttct ttctttttag cagtaccagc tatggtggct tcttcaggaa
     6361 aagatttatt tgaatttgat tattcaataa tgacaccaac attgtggatt gctttggtag
     6421 ttgggtttat agtagctttt attgtatcaa taatagttat ggaaaaattc gtgaattttt
     6481 taaagaaaaa accaatgaga gtgtttgctg tatacagaat aataatgggt gtagtacttg
     6541 cagtattagc ttttactaac ataattagtg tatagaaaaa atagatttat catctataaa
     6601 aattaaacta agataaatta taattaacaa ttatatatat gttatttcaa aaattaaatt
     6661 aggaaggata gttaaataat gaaaatttta actgtaaata atttaagtaa agtatatggt
     6721 aaaaaaataa ttttcaacgc attaaatgac ataaatttta gtatagaaga tggagaattt
     6781 gtaggaatta tgggtccatc tggtagtgga aaaactactc ttttaaatat gatttctact
     6841 attgacaagc caactacagg aactatggaa ttaaagggca agaatccttt attgttaaga
     6901 ggtgaggaac ttgcattact tagaagaaga gagttaggtt ttgtatttca ggattttaat
     6961 ttattggata cattaactat aggagaaaat atagtattgc cattgacatt agataaggtc
     7021 agtgtaaaag agcaagatga gagattgaat gaagtatcta caatattagg tataaaagat
     7081 ttattaggaa aaagaacttt tgaagtatca ggtggtcaag ctcagaggac tgctattgct
     7141 agagcattga ttaacaatcc atcaatactt ttagcagatg aaccaacagg taatttagat
     7201 tcaaagtcat caaaggttgt aatggagcta tttcaaaaga taaataaaga aaataaagtt
     7261 actactatga tggtaactca tgacccatta gcagcgagct actgtagtag aatcttattc
     7321 ataaaagatg ggtctatata taatgaaata tataagggta gtagtagaga gcagttttat
     7381 caagagataa tggatgtact tacattattg ggaggcgata actaatgaat ataaaacagt
     7441 ttgctattaa caatatttca agaaatatta aagcgtattt aggatactta gcaagtattg
     7501 ttatatcatc ttcactgcta ttttctttta ttatgtttac aagtcatcca gatttagatg
     7561 tatctcaatt tcctgattat ttaaaagaag gtctcaagat gagtaagata attgcatatc
     7621 tattcttatt cttctttgtg ttttattcag ttagtgtgtt tcttaaaagt agatataaag
     7681 aatttggaat actttatatt acaggtatat caaagcgcca agtaatgaaa ttaatcttta
     7741 tagaaaatat gataataaat atagtatctt ctgtaattgg aataataata ggcttaatct
     7801 ttgcaaagat atttttagtg attatgtcta cttttttaga attatcacca ttaaaattct
     7861 atataccatt taatgcgata ttgtctactt taatctattt tatgatttta gctgtattaa
     7921 catcaatatt tacttctttt atagttaaag aaaatcaggt gttgaagtta cttaaaggta
     7981 caaaaactcc aaaaccagag cctaaaacat cactaatgct tgctatatta agtatttgtt
     8041 tgtttataat agcgtattat aatgctatta cctcaaccga ccaatataca acatatctgc
     8101 gcttagttcc agttacatcc cttactatag tagcaactta cttgttcttt tctcaattta
     8161 gtgtattttt tatgaagaag ctaaaactaa acaagagatt ttatagaaaa aatacaaata
     8221 tgctttggat ttcgaacttg atttataagg taaaggataa tgctagaata tttttcttga
     8281 taactataac ttctgcagta gcatttactg ctataggtac agtttattcc ttttggaaag
     8341 atgtagagcg tcaaattaat ttaatatatc ctaacacaat atattattca acaatgacat
     8401 tgcataatga ccctaaaaag cctgatagca aagaaaaaga caaggaaaga atgatgttta
     8461 tagaaaataa tctaaaaaaa gagaatgtag actatacaag gactaatggt gaatttaaaa
     8521 ctgtatttcc caagaaaagt gattttaatg tcagaattat gaaagaatct aagtacttag
     8581 aaattacaga aactataggg gttaagtcta taagttttga agataatgaa tgtatatcat
     8641 tattatcatc aagtttacca ggaaataata acgtcaaaga aaatattatt gtagggaata
     8701 ggggtttaaa ggtggcaaaa caagtggaag aatgtgttat gccagcatat tacaaaaata
     8761 tgtatgtggt aaaggataat ttttatgata gtatagaatc aaaatttata gtggatagat
     8821 ttactgcatt tgaagtagag gattcaagta aaactataga gatatgcaga gaatttgaaa
     8881 ataaatttaa taatgagtca gggatgcaac catatttgtt tttctctaaa gaattaatga
     8941 taggaactgg gaaattaatg tattctacat ttatattttt agcaatattt ataggtttaa
     9001 ttttctttgt tactacaagt agttttttat ataataaatt ttatatggat tgccaagttg
     9061 ataagaaaaa gtatgaacag ttaaataaat tgggtatgac atataatgag attaaaaaag
     9121 cttcaacaat tgagattgga atagtatttt tgtttcctta tgtagtggct gttatacatt
     9181 caatatttgc acttactgca cttaaaaatt cttttgatat agaagtagca tcatcagctg
     9241 ttttggtaat gggaagcttg ttcttggttc aaatagttta tttttttatt gtaagaaata
     9301 attatttaaa agagataagg ttaaatttag tcaattttta gttactatta ttaaatgata
     9361 ataaaaaggg tttaaaatta attaatatta ctggaattat agatgtttat tttttcagta
     9421 atatttttta ttgtattaac tattaaaatt ttgtacaaat ataaaatata atatggtaaa
     9481 tgtcaaaatt ataaaaaaat tctacttata taaaatataa agaggttata tgtacatatg
     9541 tgtagaaata aataataaaa atatcaatta atgttattag gggggggtta ctcattagta
     9601 aattagctaa tacacttaga atgatgatat taattcaaag cagaggcaaa atgaaatgca
     9661 aagaattatc agaagagttg gaagtaagtg aaaggcaaat aaaaagttac aagatgtatt
     9721 tagagcaagc tggtatattt ataaattcaa cacctgggat ttatggtggt tatgaaatag
     9781 ataaatgtaa tagcatatca ttaattaaat tattagatag tgaagtttca atttttatat
     9841 caactaagtg gtagataagt agaatattag caatatcaac gagtatttag aaacttattt
     9901 tatattaata aagcagtata taaaattgta gaatcaacaa tagcatatac taaaacatcc
     9961 accttatatt aactaaatca tatctaaata aaatacactt acctacaaac attataaaat
    10021 caatacaaaa atgaggtgaa acaaaattta tgataaagaa attaaacagt aaagacataa
    10081 ataaaatcat ggaaatatgg gaaaaaagta caatcaaagc acatgacttt ataagtaaag
    10141 aatactggca aaataactat aatactgtta aaaacgaata tatacctata tcagatacat
    10201 ttgtatatga tgatggagat gaaataaaag gatttataag cataatagat aaaaacttta
    10261 taggagcttt atttataaag cccaaatacc aaaatctagg tatcggaggt aaacttttag
    10321 attatgcaac taaaaaatat aaaagtctaa gcttagcagt atataaagat aataaaaaag
    10381 cagttgtgtt ttataataaa aaaggtttta atatagtaaa agaacaagta aatgaagatt
    10441 caggatttaa agagtacata atggaatata gtaaataata tgattacatt ctattaaaag
    10501 gataattaat ttattttcta gttgattttt taagagtaat ttttaaaata gactatatga
    10561 agataaagat atttattata gatgtaagtt aatcagaatt gatatagcta caaaagagga
    10621 aaataaaatt gtatggtgta tttcagattt aacaaattta ctatttaaag ataataagtt
    10681 tgatataata ttaaagatac tttcacctat aaactataga gtattgaaag aattttaaat
    10741 aattatggaa tgctaataaa ggtttgttaa aatttaataa ttattagatt atagaaaatt
    10801 gccatgaaat tagttcatca agaactagtt tcatagcaat tttaattctt ataagtgtat
    10861 ttatttttac agatttaaaa aacttataca gtatctaata atgtaatctt attactttag
    10921 cctataaata tttaaatctt aaaatgttct ctttgagata agcttatttc agagtcacaa
    10981 tttccacaac agtaaaatgt tgagacatct ttaaattttt cagtattctc atcaaagttc
    11041 atttttttct ttgcaataat tcctacctta gttaaattag aaccacaatt attacagttt
    11101 tgaattttgc ataggttata tagtttatca agtgtaatat cttctaataa ttcatcacaa
    11161 ttacaacatc tgatagaaga aacttcacta cacatttcaa taggttcagc ttctttagaa
    11221 gaaaatttat atgttatgtc atttgatact ttataacctg tatcaaatat attagaatta
    11281 caacttggac ataagtactt tggaagatat atatcttttt tttcaaaagt atcactacta
    11341 atattacagt ttaattcatc taaaattggt ctttcatcta ggttattacc tttatttaag
    11401 cttagaagat aattataaat ttttttatct tcattctgtt ttgatgtatc taacaaatgc
    11461 tccattatat tagtgtattt tttgtttttt gaaggcatgt ttaaatccag ataattgttt
    11521 tcaatagcaa agcatactat attgaaatag ttatatttaa ctgcaaaaaa taataacatt
    11581 tcatcaaata ctttatctat tattttgtca gaaatactta tccaaaaatc tggtgactct
    11641 aagtaaactt tcctactaag ttcatctaac tcttcatcta tattattttc attaactttc
    11701 atatcatcac ttccttttaa atatcttgtg tataaactat ttgttactat tttttatata
    11761 aaataaaatc ttatat
//
LOCUS       DAYATN010000119        11659 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.119, whole genome shotgun sequence.
ACCESSION   DAYATN010000119 DAYATN010000000
VERSION     DAYATN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.119"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            70..768
                     /locus_tag="ACGI8A_002906"
     CDS             70..768
                     /locus_tag="ACGI8A_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180896.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            946..1836
                     /locus_tag="ACGI8A_002907"
     CDS             946..1836
                     /locus_tag="ACGI8A_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180897.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2229..3185)
                     /locus_tag="ACGI8A_002908"
     CDS             complement(2229..3185)
                     /locus_tag="ACGI8A_002908"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0180898.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3888..4883
                     /locus_tag="ACGI8A_002909"
     CDS             3888..4883
                     /locus_tag="ACGI8A_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0180899.1"
                     /translation="MSIIELNELTKAYRRFEKKAGFMGSVKSLFNRKYTEKTAILSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4870..5724
                     /locus_tag="ACGI8A_002910"
     CDS             4870..5724
                     /locus_tag="ACGI8A_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180900.1"
                     /translation="MSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFDVVV
                     NMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSEEIC
                     NRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFTSNP
                     VQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFKMIIENIVEFITGTMIPLVLL
                     PETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYKRMR
                     TLFDGVGI"
     gene            5724..6515
                     /locus_tag="ACGI8A_002911"
     CDS             5724..6515
                     /locus_tag="ACGI8A_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HFH0180901.1"
                     /translation="MKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLMF
                     NSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYITK
                     PLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLLF
                     YDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMST
                     IPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6800..8179
                     /locus_tag="ACGI8A_002912"
     CDS             6800..8179
                     /locus_tag="ACGI8A_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0180902.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            8627..9340
                     /locus_tag="ACGI8A_002913"
     CDS             8627..9340
                     /locus_tag="ACGI8A_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0180903.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(9612..11150)
                     /locus_tag="ACGI8A_002914"
     CDS             complement(9612..11150)
                     /locus_tag="ACGI8A_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFH0180904.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         4247 a         1329 c         1783 g         4300 t
ORIGIN      
        1 tgatgataaa atataattaa gttaagttgg tttttataaa cataaattta ttttaaggag
       61 gtgaaataaa tgaaaatatc tagtcaatat agaagtcaat attcatttag atatgaaagt
      121 aatataaata atacaagaat aaatgaaagt atggttaaga aaaatgaaac tgtaggaaaa
      181 gacacttatt tatctaatat tatgaaacaa aagcaagaac ttaatgatag aattagagat
      241 ttaaaatata gacaagaggt ttatactaaa aaaataaatg acgcaattaa gaacttatgt
      301 aaatcagaaa taagagaaac aactaataat ttttctaata tagaaatagg tattaaaaat
      361 agcattatag aagagaaaaa taaaagtaca atgttagatg aaaattcaac ttatctaaat
      421 acaaatgatg aaaaagaatc tttaatcact aaagagtcta atgaaaaaat tgaagaagaa
      481 atattaaatg atgaaaaatt agaagagtta gaacaaaaaa aggattataa agaggattct
      541 aataaaaaag agaaagtatc tgaggactta tctttagtag gtaaaactcg tgaagagctt
      601 gaaaatatgc ttaaaaattt tataaattta acacaagaag aaataatgaa acttgagtcg
      661 agaatagaaa agttagataa aaatgctgaa gaatacaaac aaaattcaaa gactaatata
      721 tttgataaaa cagatgaaca aaaaaaacat ataaatgtac tgatttaaac aatatgtttt
      781 aaaaaaataa gtttattata taatgaaaat ataaaggtac aatttcaata cacttctatt
      841 aaaccaataa tttatgaacc agacttaatt tcaatttact aataaccata tatttatgag
      901 ttaatagttt tatgttttaa aaagaattaa ataaggggga gtagtatgaa gaagaatata
      961 ttaactacta tgattaccat gattattata ttgatgatga gtatgacagt aatggcagag
     1021 ccatatcaag aagatgaaac atctagcttg aaaaaacaga gtgcaaatga acgtaatcag
     1081 actaatatag gcacaatgga tggcagagtg caaacaaatg caataagtaa aggtgctaat
     1141 tatgacccta aatatcctct taaaggaatg ttaacacaac ttggtcttaa tacaacaggt
     1201 aaagagggat gggatttact ctatggagga aatcccaatg gagttgagct tcgagcagat
     1261 ggtaaatatt attatataaa taagggtatg gaagggctag gattttatgc ggcagcattg
     1321 tcaggtggct catatcagag aataggcttt ggaaaacttg accctataga agtaatagct
     1381 gatggaaatt ggatttctta caaacaagct caagatacac ttacagtaat caggaatttt
     1441 ttaaatagct ttgactggag aaatgcatct gagatggaaa gagctaatag agctgcaaaa
     1501 ttggtaaccg aagcaaaata tgcagactct aaatattgta acatagtcta tggtaatctg
     1561 gttgataaac gtggtgtatg tggaagtttt gcaagtagct ttcatttatt aacaaggtta
     1621 atgggaatgg attcactatc tatattaaat ccatcattaa atcatgcttg gaactatata
     1681 caaattgatg ggaaatggta taggtttgat gggtctgaaa tatcagcttt tggtggatca
     1741 ttagactttg attaccgtaa attaaaggat gcaacaagag agatgactac ttactatgat
     1801 gctaaggcat tgtctatatt aggatttaat caataaaaat agggggaggc ttcttataga
     1861 gccttttttt attgatttga aatttaataa ggaattttat atgaaaattt aatcaataaa
     1921 agcaaaaaag gctctttata aagcctttct aagtgttttt ttaagtaaaa ttttttatat
     1981 caacatcagt tttaatcaaa acatataaca ttttggactt acttttaaaa ataaaattat
     2041 taaaatataa tattaatcat gtttttagtt tattatattc tataatagtg gtcatgttga
     2101 ctattgtagt tttattaaga atagttggtt tatataaata tatgcaaaga tagcttgttg
     2161 tttactttac ttattagtat ataggacaag atactctaat aaatttgcct attttaatat
     2221 cttttgtact aaaaccaact aaagtttcct ttattatttc gttttgaata atacaaagca
     2281 taatccgatt gtttgtataa agactggaaa gtatcagagt tattttctaa tttagatata
     2341 cctatagaac aagaattact ctctaggcat aattctaaaa ccttctttgc cttattttca
     2401 acattctctt ttgattgatt actatattca aaaacagcaa attcatctcc accaagtctt
     2461 ccaataatag catcatttac gaatacttta gtcaaaatat ttgaaaaatg tattagtatt
     2521 tcatctcctc gaacatgacc aaagatatca tttacctgtt taaaattatc taaatcaaag
     2581 atgtaaaagt atccatgtga aacagtttta agcatttctt ttatgtgtac ttcaaaaaaa
     2641 tgtctattgt atagtccagt cataaggtct ttttctgatt ttagtttaaa ttcatttgcc
     2701 attgctagtg cttcttgtgc tttttcagtt attgttttag ggtaatactc atctttcatt
     2761 tggtcaaagt ttggaataga atggtgaaca tggataagtt taatcatatc atctgtaact
     2821 ctgtatacca tagtaaatcg agtatccatt tctacataga tatctttttc taggtaatct
     2881 ttttctatta cccaaagagt accatatact ataaaagctt cgtctgtgac tttggtacat
     2941 gaataccact catctataat ctcaaagtct atttgctctg attctgtttg gtctttttca
     3001 agacctataa taaattcttc taatgtttcg taaaattcat gttttcctgt tccaattaat
     3061 gcaaggttgt catctaataa ggaaataata aatttaaggt tttcttcttc tggatttaaa
     3121 atataaatat gccaaatttt ttttgataaa tctcttaatt gtttttgtat tgtattattt
     3181 tccatagatt acccccttaa gatttacagt ttatatttta atgtattata agttgaactc
     3241 ttattacaat attattataa tatagaacta aagaaattac atcctgtata ggtaaaagta
     3301 tatcttttac aataaaaata ttacagtaaa atgaccagtt ttaatatacg tattatattt
     3361 ttaatttata aacgaatatt ataaatgaaa tttaaattca accttgaaga aataagctaa
     3421 atgtattagt atattaatcc atgcttgaac ataaataacc tttatgatat gatatcaata
     3481 ataatattat gagaggagtg ccttgatgtc ggagttatat aatggtaacc aacaattaaa
     3541 tagttggaga cacatatatt ctgtataatc agaaatttca agtgtatttt taatacattt
     3601 aatttttgtg tcttattctg cctttataga taactgacat atgacaacaa atagtattta
     3661 aaatatatat tactttgtta aattatggtg tagtaggctt taatctgcta cacttttatt
     3721 aattttgtat agtattattt tgataactat tttgaaatag agagcattaa tttttctagt
     3781 ccacaggagt tttctgtttt tgtgctgtta aatataagca cagaaaaagg cagatatata
     3841 cctgtggact ttttttatat aaattatgaa atatggaggt agttaaaatg agtataattg
     3901 aacttaatga actgactaaa gcttatagaa gatttgagaa aaaggcagga tttatgggga
     3961 gtgtaaagtc tttatttaat aggaaatata cagaaaaaac agctatattg tcatttgatt
     4021 taaagataga agaaggagaa tttgttggat taataggtca aaatggagct ggtaaaacta
     4081 ctcttattaa gatgttaact ggtattattg caccgtcttc tggagaaata tctgtaatgg
     4141 gttattaccc taacaaatta gaaaataatt ttaaaaaaca atatgctgtt gttatgggcc
     4201 aaaaaagtca actattcttt gaattgacac cagcagatac gtttctttta tttaaagaat
     4261 tatatgatat atctaatgaa gaatataaaa aaaacttgga ttactttatc gaattatttg
     4321 aggtatctga ctatttaaat gttcaagtta ggacgctttc tttaggagaa agaatgaaaa
     4381 tggaactaat agtagccctt ctacataatc caaaaatatt atttttggat gaaccaacta
     4441 taggcttaga tgctatagcc caaaaacaaa ttaggtattt cctaaaagaa gtaaatgaaa
     4501 caaaaggaac tactattata cttacttctc attacatgga agatattaag agtctctgta
     4561 gtagaagtat tgttgtagaa aatagccgta aaatatatga tggttctaca gagaaactat
     4621 ttaatcattt tcaaaaatat aaaaaaataa cagttagttt ttccaatgaa actaacattg
     4681 tatttccaaa agattgtaaa gttgttgaac aaacaaatca taagatatca gtaattgtgc
     4741 caaaagaaag aggaaatgaa acgattaaat ggatttttga taaatatgat gttaaagatg
     4801 taatgattga agaagaagat ataggtagta ttgtagagag aatatataag aaaggaagtg
     4861 atgagtatta tgagcaacta taatgaagca agttcaaatg attcaatgtt ttataaattt
     4921 aaacagagta ttagaaggta ttatgaagta gccaaaatta actttaaagt gcaacttatc
     4981 tggagatttg atgtggtggt taatatggtg tttacgattg taaaaatttt atttgcttat
     5041 attttatgga aagcaatttt tggtgaaaat aaaatagtct ctggttttac atttaactct
     5101 atgttgtctt actatataat aagctcattt ttagcaggta ttgatatgtc aaagagtacc
     5161 agtgaagaga tatgtaatcg tgtaaaattg ggcacctttt caaagtacat gattattcca
     5221 attaatgttc aaaattattt tattgctaaa aattttggaa tgacatgttt ttattttgta
     5281 tttaatatga tttctgcatt tgtatggatt tttatattta aaatagattt tgcctttaca
     5341 tcaaatccag ttcaaatatt aatttcaatt ttgatgattt ttctagggtt tatttttatg
     5401 atgaaactcc acttctttat tgggattttg tcatttaaat ttcttgagat tagttttttt
     5461 aaaatgataa ttgaaaatat tgttgaattt attacaggaa caatgatacc acttgtactc
     5521 ttaccagaaa ctatagtaac agttatgaag gtatttccat tttattatac aacatatttg
     5581 ccatctatgt tactaatagg tagaaacagc aatgaggcaa tacaaggatt aattattcta
     5641 gcattatggg ttttattttt ttctgtagta aataaagtta tgtataaaag aatgagaaca
     5701 ttatttgatg gggtgggtat atgatgaaaa aaaatttgaa aataatattt atgttattaa
     5761 aaatgaaatt atcaaagatg atggtattta gattcgattt ttttggagca ttttttgtag
     5821 atagttcatt atttatatta caacttctaa tgtttaatag tatatattct catgtagact
     5881 caataggggg atggcaacaa ggagagatgt taatatttat aggaactttc tcattaataa
     5941 atgcgataaa tatgacgatt ttcttttttg gtatttatga tattccacga aaaatccaag
     6001 aaggagaatt agactactat attacaaaac cattagaccc actatttagg ttaacatttg
     6061 aaaatattaa tattggttca tcaccattaa ttctagcaag tatacttatt gttttatatg
     6121 gaatatctga gttaaactca gagataactt tagttatgat aataatatat attttcatgg
     6181 tgttattgat gacattactt ttttatgatg tatgtatcat acttagaaca gtttcatttt
     6241 ttgttatatc atcttctgcg ataatgagat tagaagataa tttattgcca atgaatatga
     6301 aaattcctgg tgtaatatat gaaggtgttt ttaaagtatt attctattta atcttaccat
     6361 atggaattat gtcgacaatt ccgactcaaa ttttggctgg gacaataaca attaatggat
     6421 taatatactc aattttaatt gtatttttgt ttacattgtt tatgtggaga ttttggaagt
     6481 taggtatgag acattataaa agtgcaagta gttaataaat tagtaaaaat agacaatgta
     6541 taactatata taaatacgtt tcatatgaca ctaaactatg tgaacaagta tttgcgctaa
     6601 aggcactgaa agctataaaa ttattaaagt aaggatttaa aagtttcttt agtagaaata
     6661 taattgtgta aaaaagcaag attaattaaa actatatcca aataattaga tataattctt
     6721 atgcagaaag gaggtctgta aatgtctaat tgtttggaat atattttaaa aataattaat
     6781 gtacagaaaa ataaacaaaa tgagtgatgt atattattca gaaaatcatg aacaagataa
     6841 tctttatgag caagataatt tactggcaca aagatttaat ttatttactc tattgaagtt
     6901 tgcatttcct actattttta tgatgatttt tatggggttg tatacaatcg tagatactat
     6961 atttgtatca agatttatta atacaaatgc tctatcagca attaatattg tatgtccaat
     7021 tattaatctt atagttggaa ttggaactat gcttgccact ggaggaagtg ctattgtagc
     7081 acgtaagatg ggaaatggtg atattgaggg agcaaataaa gctttttctt taatcgtatt
     7141 atcaggagtt gtaattgggg taataattac tggtataggt attgtattta tggattatat
     7201 cattatatgg ttaggagcaa gtgaagtttt aattaaatat tgtcgttcat atctttttat
     7261 tatcttggtt tttgcttctg caaatatact acaagtatta tttcaatgtt tatttgtaac
     7321 agctggaaag cctcaatttg gattatggct agtgttaggt gctgggtgtg ctaatataat
     7381 tcttgactat gtgttcattg ttctgttaca aatgggtatt agtggagcag cacttgcaac
     7441 tggagtagga tacctaattc ctaccgtttc aggtatggtt ttcttttcaa aaaataaagg
     7501 gttacttaaa ttttgtatac ctagactaga taaaagaata ttatttgaag cttgttttaa
     7561 tggttcatct gaaatggtgg gtcaactatc aacagcagtt actacttttt tatttaatgc
     7621 tatgatgatg aaattaattg gtgaagatgg ggttgcagct attacaataa ttatttattc
     7681 tcaatttctt ttaacttcgc tttatatagg tttctcaatg ggtgttgcac caataataag
     7741 ttataactat ggaagtgaaa ataatacgca gttaaatcat gtttttaaga tttgtatatt
     7801 atttatttca gtagcatctt tattggtatt tatcttttct atatcatgtt caccaatcat
     7861 tgtagagatt ttttctcaaa gaggaagccg agtttatgaa attgcaaggt ctggatttta
     7921 tatattttct tttagtttta ttttttgtgg gtataatatt tttacttcgt ctatgtttac
     7981 agcattatca aatggaaagg tgtctgcaat attatctttt ctacgtacat ttggatttat
     8041 aactataggt ttattgatat tgccaaatat attaaatata aatgggatat ggttagcagt
     8101 accaagtgct gagattttaa ctttattttt aaccattttt tgtatatgta agtataagaa
     8161 tagatataaa tatatgtaga tattaaataa atagtttagg tagatatttt atgttttaat
     8221 aagtaattca attagtggga gatatatact attgaaaatt aaagttataa ttgatataaa
     8281 gaattaataa aaataacata taaaagttta aaagggagtt gaacttaaag tttgattaga
     8341 aaaagtcaat tttaagacaa ctccttttat tttactattg tattaatctg tatctgtaag
     8401 tgtattagtc taatattata aactttggat gaaattaatt ttctataggt tttaatgtat
     8461 catacacttt attttaaaga aacttatctt aattttcata gtgattgaat ttgtctatta
     8521 gagatgttac tattagtaat gtaaaagcaa gagtagtgaa tataaaaaac aagatagtga
     8581 tactgcaaga ttagaatata ataaattttt atggagagat ttcaatatgg atacatgttt
     8641 taataaaaaa atattacttg ttgatgatga aaaggatata gtagatttaa ttgaagaagt
     8701 actcataaat gatggtttta aaaacattat taaagcttat aatggtctag atgctatttc
     8761 gctgtgtaag atagcatgtc cagatgttgt cattcttgat ataatgttac cagatataga
     8821 tggtatagaa gtgtgtaaaa aaattcgaga gttttcatac tgttctattc tttttttatc
     8881 atcaaaaaat gatgatattg acaaaatact aggtcttagt agtgggggag atgactatat
     8941 aacaaagcca ttcagtccaa gagaaattgc ttttcgagtt aaagcgcaat tacgtagaca
     9001 acagtatcaa agcattgtac catcagatag tgaagttatt aaaataggag atattactat
     9061 tgatatagaa ggaaaccgtg tatataaaga tagaaatgag attgagttaa ctggaaggga
     9121 gtatcatctt ttaagctaca tggctcaaaa tgtaaataaa attataggaa aagaaagact
     9181 ctatgaacaa gtatggggag tatatagtag tatttgtgac aatacaatta tggtgcatat
     9241 acgacatata agagaaaaaa ttgaagataa cccctcaaat cctaaaatac ttataacagt
     9301 taaaggacta ggatataaac ttgtgaatag aatcgattag aatagatttt aatattaatg
     9361 aagatatctc aagttgaatt tttagttaat gaggcattct tttatgggta aaatcaaata
     9421 tattttcgtt atctcaacaa tgaagaagag gtctatctct attttgagat agcctcatta
     9481 agtttatatc ctggtaatgt actttatctt ctaataaaat ttttttagtt tatttgcagt
     9541 gaaacattaa ctagaacatt aaaatttgca ttgaaatact aattaaataa tattgtatat
     9601 tgtgtataaa cttatactaa ttgtttttct atattaaata ctttatcaat ccaaccttta
     9661 taaaattttt cttcatggga aactaggatt atacttcctc taaactgttt caaagcattt
     9721 tgtaaagcat cctttgtctc tgcatcaaag tgattggttg gttcatccag gataagaaaa
     9781 ttacaaggag ataacatcat acaacataat ttaaccttag attgttcacc accacttaaa
     9841 gtagatacac tccttgaaac atgctcttcc ttcacaccac atctggctag atgacgtcta
     9901 atctcttttg tatttaattt tggatattta tctgctacta tttgaagtgg ggttttatca
     9961 ggattttccc attttaaatc ttgctcataa taaccaattt taacttgttc tgaaaactgg
    10021 aaatctccag atatacgagg aatatctttg actaatgtct ttaacaaggt agatttaccg
    10081 attccattaa aaccagttat aactattttt tgacctccat caacagagaa atttaatttt
    10141 ggaagtaaag aatagtagta tccaacttca agatttgtta tagtaagagc attttgagcc
    10201 gatatttcaa tttctgaaaa ttgaatattt ggttttcctg taaaactagg aggagctatt
    10261 ctttctattt tatccaattg tttttgtctt cctcttgcaa tttttgaatt tactccagct
    10321 ttatttttac gtataaatgt ttcttctttt tctattttct tttgttgtgc ttgatacctt
    10381 ctaatataat cttctcttaa atgtgatttt tgtttaagga actctgaata ttttccatga
    10441 tatttcttaa tcatgccaaa ttctatatca agaatacctg tagaaatttt ttctaaaaag
    10501 tcaaaatcgt gggaaactac aataaatgcc ccattaaatg tatttaaata atttgagagc
    10561 cattcaacat gttctttatc taaaaagttt gttggttcat ctaataatag aacatcatgg
    10621 ttagaaagta gtaattttgc taaaattacc tttgcccttt gaccaccact aagctctcca
    10681 acaacacgat tcattcctat tgaatctaat ccaagaccat ttgctatttt atttatttca
    10741 ttatctatag aatagaaatt acttgctatt agttgttcct gataatctga tgctttgatt
    10801 agatattgct cattttcaga tatagcactt ttttgataga gtaaattcat ttccttttct
    10861 attttataaa gttcttcaaa tgcagtgtgt aaatagagtg atattgttgt atctctattg
    10921 atttctgcat attggtctaa atggcctata ttgatattag gttgccactt gatttctcct
    10981 gaatctggaa caacttctcc caataaaatt ttaattaatg tacttttacc tgtaccattt
    11041 tggccaacta cacctatatg ttcacctttg aataaatcaa agttagcttt ctcatacagt
    11101 gatttatcca taaaacattg tgataaatta gttactttta ataaactcat aattcaaact
    11161 ccttttcttt tattaaaaaa tttatggaaa taaaaaaagc agaggaatgg cattgtaaaa
    11221 tgccgacctc tgcttttgga aaaacaaaag gtcatagaca agcattgttg tctatgacca
    11281 gaaaatctat attatctatc ggcaaatagg taattttaaa taagcatagc caaaaaacta
    11341 tactaaacct atatttgccg atgaagatag aaagctttaa gctttcagat taacctctac
    11401 acaacgcttc atcgtataca tttgtgtaga gttaagtttt ctaacaagtt taactaattc
    11461 tatataaatc ataattattc acatcctaat cttagtatta attctagttt atctgtattt
    11521 aaattgtact tttgtacttt gtggattatc ttatttattt aaaatatgtt ttattaatta
    11581 tagcttaagt tatgttatca taatacttag ttatagtcaa taagaaagtt atatttacta
    11641 ttattatatt aatttagta
//
LOCUS       DAYATN010000120        11630 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.120, whole genome shotgun sequence.
ACCESSION   DAYATN010000120 DAYATN010000000
VERSION     DAYATN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.120"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            354..2264
                     /locus_tag="ACGI8A_002915"
     CDS             354..2264
                     /locus_tag="ACGI8A_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFH0180905.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNVGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(2327..2950)
                     /gene="ytaF"
                     /locus_tag="ACGI8A_002916"
     CDS             complement(2327..2950)
                     /gene="ytaF"
                     /locus_tag="ACGI8A_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HFH0180906.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            3138..3782
                     /locus_tag="ACGI8A_002917"
     CDS             3138..3782
                     /locus_tag="ACGI8A_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0180907.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            3996..5054
                     /locus_tag="ACGI8A_002918"
     CDS             3996..5054
                     /locus_tag="ACGI8A_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HFH0180908.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRSIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            5158..6342
                     /locus_tag="ACGI8A_002919"
     CDS             5158..6342
                     /locus_tag="ACGI8A_002919"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFH0180909.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMIVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            6549..7262
                     /gene="rpsB"
                     /locus_tag="ACGI8A_002920"
     CDS             6549..7262
                     /gene="rpsB"
                     /locus_tag="ACGI8A_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFH0180910.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            7341..8252
                     /gene="tsf"
                     /locus_tag="ACGI8A_002921"
     CDS             7341..8252
                     /gene="tsf"
                     /locus_tag="ACGI8A_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFH0180911.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            8337..9041
                     /gene="pyrH"
                     /locus_tag="ACGI8A_002922"
     CDS             8337..9041
                     /gene="pyrH"
                     /locus_tag="ACGI8A_002922"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFH0180912.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            9067..9624
                     /gene="frr"
                     /locus_tag="ACGI8A_002923"
     CDS             9067..9624
                     /gene="frr"
                     /locus_tag="ACGI8A_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFH0180913.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDALLSKKEKDIMEV"
     gene            9627..9830
                     /locus_tag="ACGI8A_002924"
     CDS             9627..9830
                     /locus_tag="ACGI8A_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180914.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            9859..10593
                     /locus_tag="ACGI8A_002925"
     CDS             9859..10593
                     /locus_tag="ACGI8A_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFH0180915.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            10606..11385
                     /locus_tag="ACGI8A_002926"
     CDS             10606..11385
                     /locus_tag="ACGI8A_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFH0180916.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         4888 a         1171 c         2034 g         3537 t
ORIGIN      
        1 gatgacaata taagtgattc ccaaatataa atgaaagtat aagcttattt ttactataaa
       61 aattttcact gtacaaaaac attaatgaca ttatttttat tgacataaat atataattta
      121 tggaataata ttagaagcac tgatataagt gtttaaataa gaatagaaaa ggagaagtgt
      181 tttatgaaaa aaaatactaa cattaatgag tccggagtta aacttcttaa taaataagga
      241 gtttgatata taataactcc ttatttatta agttgttcgc aaaataacta ttatcaaagt
      301 aaatgatagg gtgatttaaa agacgatgac ttataaataa aggagaaaaa ataatgaata
      361 gtaatacatt tgaaagatta caactaaatg aagttaaaga cttaataaaa atacattgtg
      421 taagttcgct aggaaaaaat ttaatagata aattaactcc tagtgggaat attggagtag
      481 taagaaggaa attagcagaa aataaagaag ctagaaagat aattgaaaat agtaatcaca
      541 taccacttga aggattgttt aatgtaggtt ctacaataga taaaattgaa aaaggaatga
      601 taatagaacc tgttgaactt gtaaatattg aagatttttt aagaggttgt aggaagatga
      661 aagcttttat gttagaaaaa gaattttatt cacctacatt aagttcttat gccttaaata
      721 taacagaatg taaaagtata gaagatgaga taaattactg tataaaatct aataaagttg
      781 attctaatgc tagtaaagaa ttaaaaaaga taagacgaaa tatagaaatt acagaaggaa
      841 agataaaaga tagattaaat aaatttataa catcaactgt aaataaaaag tatatacaag
      901 agtttataat aagtaaaaga aatgatagat atgtaatacc aattaaatct tcttataaaa
      961 atgaagttaa tggtactata ttagatacat catcaaaagg aaatactgta tttattgaac
     1021 ctattagtgt ttcaaattta agtacagagt taactatgct aaaagctgat gaaactattg
     1081 aagaatacaa aatattatct tatttaacag aacttatatt tgagaagata agtcagataa
     1141 aattaaatat agagatactt tcagaatacg atatggtatt tgcaaaagct aagtatagtc
     1201 aaaaaataaa aggaattact ccaaaaataa ataataatgg ctatataaaa ataattaaag
     1261 gtaaacatcc acttctaaca ggagatgcag tacctttaga ttttgaaata ggcaaaaatt
     1321 atagaagtct tataataaca ggtcctaatg caggaggaaa aactgtaacc cttaagactg
     1381 ttggtttact aactttaatg gttcaatgtg gatttgatat ttctgcaaaa gaaggtagtg
     1441 aatttagtgt atttgaaaaa gtttttgtag atatagggga taatcaaagt attgaaaatg
     1501 ccttaagtac tttttcatca catataaaaa atattgcaga gattatgagt ttatcaaata
     1561 attctactct tgttctgttt gatgaaatag gaagtggaac tgaacctaat gaaggagctg
     1621 gtttagcaat atcattatta gaagagtttt acaaaatggg atgtataact atagcatcaa
     1681 ctcattatgg agagataaaa aagtttgcta cattgcatcc agaatttgaa aatgctggta
     1741 tgatgtttga taaagaaacg ttagaaccat tgtataagtt aactattgga aaatctgaag
     1801 atagtaatgc tctttttatt tctaaaaaaa tgggaataaa aaatagagtt ttagatagag
     1861 ctaaagaata tgtaattgat agaaactaca atttagattt agttaaaaga agtaagctac
     1921 aatctaatat tgaagataaa aatagttctg aagtcatttt aaactataca gattataatg
     1981 taggggataa ggtaaaatta ctagatgaag atgattttgg aattgtatat aaacctatgg
     2041 acaagtttaa taatgtagaa gtattgttta atgataattt tatttcagta aatataaaaa
     2101 gattacaact tagcataaaa gcatcagaat tgtatccaga agggtatgat ttagatgtat
     2161 tatttactag ttttaaagaa agaaaattag aaaaagatat tcaaagaggt tcaaaaaaag
     2221 ctcttaaaaa gattcagaaa gaaattagag agaatagaaa ataaatataa aaagcacaaa
     2281 agaaagcaat ctaaagagaa aagtttatct ttagattgct ttcttattaa catatcttca
     2341 taaatccaat gactaacaag attaaaccac ttaaccaaga taagtttaaa tttacgtttt
     2401 taacaaacat atttcctagt aaacaaccaa gtaatactgc aattatattt gatattaaag
     2461 aaaatagaat aacttgtata taattcacac ctacaaggcc actaccaaaa ccgatagcta
     2521 atccatctaa acttaaagca agtgcaagtg taaatgcttc agcagattta agcactttag
     2581 atttacttgt atctaagaca tcaggttcga tacttacatt aaaatttaat ttaaggtcta
     2641 acatattaaa attataattc tttgagtgta tagatttttt cttaaaataa gatttaaaaa
     2701 aactttcaat tagtctagca cttcctaata aaaataaaat aaagaaacat attaacattg
     2761 ttatattttg aggtaaaaag tctctaacta ttgtacctgc aaaaagcgat attgctaaaa
     2821 tagctgaaca aactacgctg ataattatat ttgaatataa gggaacttta actttattta
     2881 cgccatatcc aaagcttgtc acaaaggcat ctatacaaag agaagtaact aataaaattg
     2941 attcaatcac agtagattaa catcctttct cttaaatttt gtacttatat tagttatagt
     3001 attgtaaaaa tggataaatt gttacagata atccaataaa ttgttgataa aattccaaca
     3061 tcatacaaca cctaaataac aaaagaatca tatcatataa taatataaaa ccaatcataa
     3121 taaagcgggt gaaaataatg gaacaaaata ataaggttgg gaatttcggt gatttgttga
     3181 agattttatt agatgaaaaa gaattatcaa tgggagaact aagtaaaaaa agtggaattg
     3241 ataaatctac tatatctcgt atagctaata ataaacaaaa gccaaatata aatcatttag
     3301 aaaagatggc aatacattta aatattaacc tagaagaact gttgaaagct tcaggatatg
     3361 aatttagaaa cagcaataat caaatattta atgcagatag tgattttagt aactttgatg
     3421 atattcttgg atttgcaaat ttaatcaatg ataagaactt taatggaaat attgaaaaag
     3481 agttaagtaa gtgtaagtta tatgtccaaa cagatgaagg taaaaaatta ctttttgata
     3541 attttaataa aaaaatagat tctattgaaa aacaaggtca atttacagat agattaagac
     3601 aaatgtatgc tgatttttgt acagatggac ttacaataaa gaaatattta ttgattggaa
     3661 gtatgctact ttattttgtt atatcaacag atgtgattcc agacttcgta ttcccaatag
     3721 gatttatgga tgatttagta gcattaaata tagttacaaa attacttaaa aatgataagt
     3781 aagttttaga atcatattta atatataaat aaatcaaagt atataaaatt tattattttt
     3841 tatatgaaac aaattaaaag cacaatgaag tagattaaaa atttaaaact aagaatctaa
     3901 tatatatttt gtaactattt aagtatataa aataaaataa tatccaaact aatacaaaat
     3961 agtaataatt gttttgtact ggaggatata tatttatgaa ttgtaaatgg atatctaaaa
     4021 tagatgagag aaaaaaatgt catagagaag cagattcttc tggatattgt atctttcata
     4081 aagaaaataa gtctgatgaa gaaatacagt tgatgatgga tactattcat aaagaagaga
     4141 taagtgaatt taatggattt gtttttgaaa atgaatttaa tgcggaagaa atattaactt
     4201 acaattataa aatactggat ttttctgaat ccatatttaa acaaaaagca aattttaaaa
     4261 aatatatttt taaaaagaat attattttta actatacaga atttagagat aaagttttat
     4321 ttaatggttg tgtattctta gaaaactgtg attttaatag aagcatattt agtaaaaact
     4381 atataaatga ccgtatattt gaaaaagtga aatttaaagg acctgatttg gtagttaata
     4441 aagtagaaaa ttttccaaga atggatggaa taatttttag catgtgtaca aaatttgtgt
     4501 taaagaatgt tgaatatgga aaaagtgagt atgagcatgg caagataaat tatagaatag
     4561 ctagaaatca agcaacaaaa attggagaat atgagatgat aggattttac tattataagg
     4621 aaagaattta tagtagtaaa ataatgaaac gttctaatta tcctactttc agtgattatt
     4681 tagttgaaaa attttttgac caaatagcac gttacacaac aggttatgga gaaaagccat
     4741 ggaatatatt attagttatt atagcaataa taagtgtttt tgctttatta tacttatttg
     4801 taggcataga aagttcaaat agcacacttg tagctttaga tataaataat ataggtgatt
     4861 actcactaag tgagattttc aaaatgtata tggatttatg gtattttagc atggctacat
     4921 ttagtacagt aggatatgga gatatggttg ctacaagttt gattgggaaa gcattggcag
     4981 gtatagaagt attttttgga gttacaatag gagcaatttg ggcatctgtg attattaaaa
     5041 gaatgataag gtaatatttg tgtaaatatg gataggtatt aattactaga tataaattgg
     5101 actaaatagg gaaaatttaa atataaatat acttaataat ccaagaaggt gaaaaatttg
     5161 aaaaatgcac taaaaaaaat tatatcatta gccatgattc tagtttttat aataccaaat
     5221 tattcatatg cagatgataa atctgtagac aagtattcaa aaacatctat acttatagac
     5281 caagaaacag gtagagtttt atatagtaaa gaacctgata tgaaggtacc attagctagt
     5341 ttaagtaaga tgatgacttt tttacttgca atagaagcta ttgaaaataa agaagtaaaa
     5401 gaaactgata aagtaaaaat agacaaatct attgcatcag taaaagggtc aagttataaa
     5461 cttaaagatg gtgaagaagt gccacttata gagcttatga gaggtcttat gattgtatca
     5521 ggaaatgatg cggcaattgc tatagcaaaa catatttgta aaacgaaaga agcatttgta
     5581 gatagaatga ataaaaaagc aaaagaaata ggaatgagta atacacattt tctaaatcca
     5641 aatggtcttc caatatacga tttaagcaac cccaaaaaac cagcaaaaga aaatatctct
     5701 actgcaaaag atatagctat tcttggaaaa tacatgtttg accattatga aaaacaagta
     5761 acagctataa ctgatatgga gacatataca aattcagata gaagttttga aaaaagcaat
     5821 acaaatgcat tattaagaat tataccagaa gtagatggta taaaaactgg atatactggc
     5881 aatgctggat attgcttatc attttctatg attgtaaata aaaatgataa aaatgagaaa
     5941 aaacgtagag taataggggt tgtattaggt actaaccata aaaataaaag aacctctgct
     6001 tcattagctc tactgaaata tggaaaagaa aatttcaata tgaaaaaagt aattgataaa
     6061 gatagcttta ttggtaagaa atatataaaa ggaatgaaag agttagaggt tacattaaaa
     6121 gcaaaagatg aactttatac aatattaaaa gatgatgaaa gtataaaatc agaagttaag
     6181 attaaaaata tagagtaccc tgttaaaaaa ggtgatacta tgggtattat aaaatattat
     6241 actaattcag gtgacttact tggaagtgta gatatagtaa gtaataatag tatagataac
     6301 gtatcattaa aaacaaaatt aaaaatgaaa tttgcaaatt aaattcaaaa aatggtataa
     6361 aatacacaaa aaaatataaa aatagaaaat atggaaaaaa tattgttatt tcttcacctc
     6421 tgtgatatac tactagaggt gaaaaaataa aataaatcac acacagcatt gggatcctta
     6481 aaaggtgcct attacatggt tttttaggga gaagatcaag ctggaggtat aaaaccagga
     6541 ggtaagatat gtcagtaata tcaatgaaac aattattaga agcaggtgtt cactttggtc
     6601 accaaacaag aagatggaac cctaaaatgg ctaagtatat attcacagag agaaatggaa
     6661 tatatataat cgacttacaa aaaacagtta aaaaagttga agaagcttac aagttcacta
     6721 aagaagtagc tgaaacagga aagccaatct tattcgtagg aactaaaaaa caagctcaag
     6781 atgcgataaa agatgaagct gaaagatgtg gaatgtactt tgttaatgaa agatggttag
     6841 gtggaatgtt aacaaaccac aaaactataa aaacaagaat aaataaatta agagaattag
     6901 aaaaaatgga agaagaaggc gtatttaatg ttcttcctaa aaaagaagtt ataaaattaa
     6961 gagctgaaaa agaaaaacta gaaaaatact taggtggaat aaaagacatg cctgaattac
     7021 caggtgcaat gttcgtagtt gacccaagaa aagaaaacat agctattcaa gaagctcata
     7081 gattaggtat accagtagtt ggtatagttg atactaactg tgacccagaa caattagact
     7141 ttgctatacc aggaaatgat gacgctataa gagccgtaaa attaataact ggtgctatgg
     7201 caacagctgt aattgaaggt agacaaggtg ctgaggaaga agtagctgaa gaccaagaat
     7261 aataatttga caattaaatg gtaagggtcg cccttaccat ttataatatg gaatagaata
     7321 agtaggagga aacttaaaaa atggctaata taactgctca aatggtaaaa gagttaagag
     7381 aaagtacagg cgcaggaatg atggactgca agaaagcttt acaagaagct gaaggaaaca
     7441 tggaaaaagc agtagattta ttaagagaaa aaggattatc aaaagctgct aaaaaagctg
     7501 gtagagttgc tgctgaaggt ctagttgcaa tagaaatgaa tgatgataat acagttgctt
     7561 ctatggtaga agttaactca gagacagatt tcgttgctaa aaacgaagac ttcaaagtgt
     7621 tcgttaaaga tgctgcttgt atggcattag ctactgataa agaagatata gcttctttat
     7681 taggtgaaac tcataaagag ggaataactt tacaagaagt tttaaataat agagttgcaa
     7741 aaataggtga gaaattagac tttagaagat ttgctaaagt tgtaactaat ggacaagttg
     7801 ctggatacat tcacggtggt ggaaaaatag gtgttcttgt tgaaatggaa acagaagcta
     7861 gagatgctaa agttttagaa ttaggtaaag atgtagctat gcaagttgct gctatgaatc
     7921 ctaagtatgt ttcaagagat gaagtagatg ctgaatacat agcacatgaa acagaagtat
     7981 taactcaaca agctttaaat gaaggaaaac cagctaatat agttgaaaaa atggttaaag
     8041 gaagattaga aaaagaatta aaagaggttt gtttattaga gcaaactttc gttaaaaatc
     8101 cagatataac tgttaaacaa ttagttgctg atgttgctaa ggcagtaggt tctgatataa
     8161 aagttgttaa agttgtgaga ttcgaagttg gtgaaggtat acaaaagaga gaagaaaact
     8221 ttgcagaaga agttgctaag caacttaagt aataaccaaa gagagaacac gcaggtgttc
     8281 tcttttttaa aaaatagaaa aaattggata tgaaggattg aaataggagg tttctgatgg
     8341 ataaaccaat gtacaaaagg gtattgttaa aattaagtgg agaagcatta gcaggagaaa
     8401 gaggttttgg tataaataat gatgtagtta atgatattgc tattgctata aaaaaaatac
     8461 aagaaatagg tgttgaagta gctgtagtag taggtggagg aaacttctgg agaggcagaa
     8521 ccagtgaagg aatggacaga actactgctg actatatagg aatgttagct acagttatga
     8581 atgcaatggc gctacaggat gcactagaaa atatagatgt tgctacaaga gttcaaactg
     8641 ctatagacat gagacaaatt gcagaaccat atataagaag aagagcagtt agacacttag
     8701 aaaaggaaag agttgttata tttggtgcag gaacaggaaa cccttacttt acaacagata
     8761 caactgctgc tttacgtgct gctgagatgg aagcagaagt tatattgtta gctaaaaatg
     8821 ttgatgcagt atatgacaag gatccaaaag tacatgcaga tgctaagaaa tttacagaat
     8881 taagttacat ggaagttata cagaaagaat taaaagtaat ggattctaca gctacatctt
     8941 tatgtatgga taataaaatt ccaataaagg tatttgagct tacaacagag aatataataa
     9001 gagcagtaaa aggcgaaaat ataggaacta ctgtaaaata atgaagaatg aaggaggagt
     9061 aaaattatga aactggaaat acataagcaa ttagaagaga aaatgaatgg gacaattgat
     9121 gctttaaagt ttgaatttgg aactataaga gcaggaagag ctaatgcaca aatgctagac
     9181 aaaataagag ttgattatta tggaactcca actccaataa atcaaatagg tgctatatct
     9241 gtaccagaac caagaatatt aatgatatct ccttgggata aatctgctat gcatgaaata
     9301 gaaaaagcga tagctaactc agacttagga ttaaatccat caaatgatgg tgaggttata
     9361 agactttctg tacctgcttt aacagaagaa agaagaaaag aattagctaa aaaagctagt
     9421 aaagctgcag aagaatttaa agttagaata agaaatgaaa gaagagacgc caatgagaaa
     9481 attaaaaaga tggaaaaagg cggagaatta actgaagatg aattaaaaaa agcacaagat
     9541 gaagttcaaa aaatgactga taaatttata aaagaaatag atgcattatt atctaaaaaa
     9601 gaaaaggaca taatggaggt ataatattga aaaaatatta taacctttta ggtcttcatg
     9661 tagatgaggt aaaacaatat tttgatgagc aaaatataaa ttataccgta aaatctattg
     9721 aaggtaaaaa ggataaagaa aaacttgtag ttcctagagt tataaaaata tctgagatag
     9781 gtgatagtgt agaactgata atcacatact tttctgactc cttagtttaa ggagtcagaa
     9841 caatattgga ggcaaaatat gaataacaac attatatatg atatagactt aaataacata
     9901 cctactcata ttgctattat aatggatgga aatggaagat gggcaaaagc tagatttctt
     9961 ccaagaactg caggacataa ggctggagta gaaactataa gagatatagt aaaagaatgc
    10021 tcaaaattag gtgttaagca cttaacatta tatgcatttt caacagaaaa ctggaaaaga
    10081 ccgaaacttg aagttgacac acttatgaac ttattatcta catacttaag aaatgaaata
    10141 gctgaacttc accaaaacaa tgtaaaagta actgctatag gagatataag tgctttacca
    10201 aaaacttgta taagagaatt aaactcagct aaggaactga caaaaaacaa tacaggagtt
    10261 aacttaaatt tagcattaaa ctacggaagt agagcagata taaaaaatgc tcttatagat
    10321 atagtaaaaa attgtgaaag tggtaaaata gatataaaca atattgatga agatattata
    10381 aaaaactatt taagcacaaa gtctatacca gatccagact tggtaataag aacaagtggt
    10441 gaacaaagat tgagtaactt tttactttgg gaagttgctt attctgagtt ttactttact
    10501 gatatacatt ggcctgattt taaaaaagaa gaactacaga aggctatata cgtatatcaa
    10561 agaagggata gaagatttgg aggacttaag taaggagatt attttatgct tacaagaatt
    10621 attgcttcac tagctctggt tccattattt ctatttgttg tatatggagg tattcctcta
    10681 tatatagctg agacagcaat agtttatatt gcattgcatg agttttataa ggcatttaaa
    10741 ataaaagatg tacatccaat attcattata ggatatttat tttctattta tttagctgta
    10801 aaaaatattt ttaatttacc attagaatat acttatgcag taatttttat tttattttta
    10861 gcaagtataa tttatatgtt gatgggcaaa aacaatgtta tagatgtttc gattacgttt
    10921 ttaggagttt tttatatagg agtattttta gattttataa ttataactat aaatggattt
    10981 gaaaaaggta gtatatatgt atggcttata tttgtgatat cttttatgac agatatattt
    11041 gcatatttta gtggttattt acttggaaaa cataagctaa ttcctaaagt tagtcctaaa
    11101 aagactatag aaggagctat tggaggaata ataggaagta cactttgttg tattttattt
    11161 ggatatttat ttggcattga tttgcttcaa ttagcgataa taggaagtat aggaagtgta
    11221 atagctcaat taggagattt atttgcatct tctataaaga gatatgtagg gataaaggat
    11281 tatggaaaga taatacctgg tcatgggggt atattagata gatttgatag tgttatatta
    11341 gttgcacctt ttgtatatag tgcgattaaa ttttttatta gataaagatt ggttggattt
    11401 aaaagagcaa tctataattg ctagtaatga aaatagctat gtaactttta aatgcagaga
    11461 tattctgcag ttattagtta catggctatt ttcattgttc aaaaatttag aagaccttgg
    11521 ggaattgtat aagaataggt attactttat aatagtatac tatatttgta gtgagtatat
    11581 tttttatact gtaactatca ttaaaaaata tttttttgtt taataatttc
//
LOCUS       DAYATN010000121        11606 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.121, whole genome shotgun sequence.
ACCESSION   DAYATN010000121 DAYATN010000000
VERSION     DAYATN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.121"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            564..1577
                     /locus_tag="ACGI8A_002927"
     CDS             564..1577
                     /locus_tag="ACGI8A_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFH0180917.1"
                     /translation="MNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYIFISDKF
                     DDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNIRLINLS
                     LSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFTLDGDDS
                     FWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEKPGITIS
                     DLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCLDIEENN
                     EKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLTELVERY
                     KNL"
     gene            1710..3008
                     /locus_tag="ACGI8A_002928"
     CDS             1710..3008
                     /locus_tag="ACGI8A_002928"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFH0180918.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3122..3367)
                     /locus_tag="ACGI8A_002929"
     CDS             complement(3122..3367)
                     /locus_tag="ACGI8A_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HFH0180919.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3515..4381
                     /locus_tag="ACGI8A_002930"
     CDS             3515..4381
                     /locus_tag="ACGI8A_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HFH0180920.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4385..4921)
                     /locus_tag="ACGI8A_002931"
     CDS             complement(4385..4921)
                     /locus_tag="ACGI8A_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HFH0180921.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(5112..5315)
                     /locus_tag="ACGI8A_002932"
     CDS             complement(5112..5315)
                     /locus_tag="ACGI8A_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFH0180922.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5606..6370
                     /locus_tag="ACGI8A_002933"
     CDS             5606..6370
                     /locus_tag="ACGI8A_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0180923.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6759..8072
                     /gene="prdC"
                     /locus_tag="ACGI8A_002934"
     CDS             6759..8072
                     /gene="prdC"
                     /locus_tag="ACGI8A_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HFH0180924.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8477..8562
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            8861..8989
                     /locus_tag="ACGI8A_002935"
     CDS             8861..8989
                     /locus_tag="ACGI8A_002935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180925.1"
                     /translation="MDNQQVSFEKKKLQRLERNLVPTSVGKWGVPNLWLGNDIVCA"
     gene            9144..11318
                     /locus_tag="ACGI8A_002936"
     CDS             9144..11318
                     /locus_tag="ACGI8A_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HFH0180926.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQTPDVEKPSDGQ
                     MPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEED
                     VKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTL
                     TFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQE
                     NQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWIILGQMDEQERL
                     KQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAGREELKPNDTGK
                     PSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLRLPDGVTAYDGDGNIIGTGEVE
                     ISTQQKFKLKSVVKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQN
                     LLSYTIEKKNGQEVNFNIGLPTDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPP
                     MDDTIINPPVPPTEDAVLNPPVPPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPE
                     LSRDDTIIKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
BASE COUNT         4686 a         1277 c         1989 g         3654 t
ORIGIN      
        1 agttgttaaa ataatattta tattagtttt tatacatact tggtataagt ttttcatata
       61 ttgattaaaa ttgatgttga tttattattt ttagtatttt ttcacctttt gattcatact
      121 ttgtagaatg gtgtattttc taaaactgat tataagacaa gttcattcaa attagcattt
      181 ttatatcata taaataattg ttccccctaa ataagattta gatgtatagt attaaaatca
      241 aaaagtataa aataaaattg atgcttaatc atagataaat attaataaaa tatacaaggg
      301 gattgtctaa taaaaagata atctcctttt ttacaaataa ttctaaattg tgatataaat
      361 ctaaaaattt tatcttaaca tttaaccatt taatgataat aattgatata ataaatataa
      421 attaaatata tggtgtactt attttatgtt tttagcaaga aatatagcat ttaaaagata
      481 aattgtgcaa cttaaatcaa agtttaagat gaactattaa catagatata ttaaaaactt
      541 ttgtaatgga gaatatatgt gatatgaata ataaagttaa ggttgcagta ttagatacag
      601 gaatagacaa agaacatgat tacttaaaag ataatttagt tggaggaatt gcttttgaat
      661 gtatgcatga ttatatcttt atatcagata aatttgatga tgaagatggt catggaactg
      721 cttgtgcatc aataataaag aaagagtatg aagatgtaga gttatttgta ataaaaattt
      781 tgagaaaaga tggtattaca aatataaaag ttcttgaaga agcattaaga taccttttag
      841 acactaacat aagattaatt aatttaagtt tatcagtcat aggagtagaa agtgttaagg
      901 gcttatttga agtttgctat gaattgttta gaaaaggaaa aataatagta tgttcactcg
      961 ctaatgattt tgacttaagt tatccagcta tgtttaacaa tgttataggt gtaagaggat
     1021 ttactttaga tggagatgat tcattttggt acaacaaaaa atatgatgtt caatgtgtta
     1081 tggatagcaa ttcatatata agttgtgata taaataattc ctatagatta ccaccaaaat
     1141 gtaatagtta tttatcagca aagcttactg gtaaaatagc taagatatta tcagaaaaac
     1201 caggcataac aatatctgat ttaaatgata agcttgaatc attagcaact agaaatcatt
     1261 gggatagttg tgattttgat gagtgtagta gaattgtaaa ctttaaattg gatttgtatg
     1321 acaaagaaaa tgctcttttt atgaaagttg ctgacgttgt tagggattgc ttagatattg
     1381 aagagaataa tgaaaaactc tttaagggta gcctttttaa taaggagata ggcttggttt
     1441 atgataattg ttttaatctg ttgaaaaagc tagagcatag atttgacata aagtttaatt
     1501 atatggacat ctcaaaatat gacttagttt caatatatac actgactgaa ttagtggaaa
     1561 gatataaaaa cttataaata aagatttgta gctaaattta atgtagaagg tattaaatta
     1621 tatgtattct ttgtcggtag attaaaagta aaaaatatac tataattaga tagcataaga
     1681 gaaaatgcca atacaggggg agttagataa tgaaatatga agaagcatta gagtacatat
     1741 cacaaactaa taaattcggt ataagattag gattagaaaa tatcggaaag ttattagaat
     1801 tattaggaaa tccacaagaa actttgaata taatacatgt agctggaact aatggaaaag
     1861 gttctgtgtg ttcttttgtt tctaatatac taagagagtg tggatataaa gttggtttat
     1921 atacatctcc atatttagaa acttttactg agcgtataag agtaaatgga caaaacatac
     1981 cacaggatga tgttgctaga ataattgagt taataaaaga aaaaatagag attatggtaa
     2041 aagaaggcta tgcatatccg acagaatttg aagtggttac tgcaatggca ttttattact
     2101 attcagaaca aaaggtagac tttgtggcat tagaagttgg tttaggtggc agatatgatg
     2161 ctacaaatat aataacaaaa tcacttgtaa gtgtgatagt atctataagt ttggaccata
     2221 caggtatact tggagataca atagaaaaaa tagcgtacga aaaggctgga ataataaagg
     2281 aaaatggagt agtactagta tatgaccaga ctgatgaagc aaaggatgtt ataaagtcag
     2341 tttgtaaaga gaaaaaagct aaatatatag aagttgattt tgatgatata aacataaaaa
     2401 aatcagatat aaattctcaa atatacgatt gtactgtaat gaaagaaact tatagagacc
     2461 ttgaaataaa attgataggc gaacatcaaa taaacaattc tatcttggca attagtgtaa
     2521 taaaatatct aaaagacata aataaattag caaatataaa tgaagaatct ataagaaaag
     2581 gacttataaa tactaaatgg ccaggaagaa tagaaaagat aaaggaaaat ccaattttta
     2641 taatagatgg tgcacataat gaagatggag caaaatcttt ggcaaaagca cttgataaga
     2701 attttaaagg tagaaaatta accttattaa taggtatgtt agaagataaa gatattgata
     2761 gtgttttaga gatattgttg tcacatttta ataaggtaat aacaacaaca ccaaataacc
     2821 caagggccat aaattcagat atattaagag aaaaagtatt aaaatatgta gatgatgtaa
     2881 catcaaagca tgagatagag gatgcagtaa attatacatt agaaacatca agtgaggatg
     2941 atattataat aagtgcaggt tcattatata tgattggtac agtgagaact ttagtaaaga
     3001 aattataatt ttataaaata agtatacaaa taaaaatata aaaaagagtt gtctaaaaaa
     3061 tggacaactc tttttatata aaataagttt ttaataattt tactgatgtt tatataagta
     3121 attatataga ttgacgtata gctttagata attggtctgg gcaagaagta ccttttccac
     3181 cacagtcaat accatttaat ttatcagcaa cttcaagagc attttggcca attattaagc
     3241 ttctaagtcc tgataaatta ccattacatc ctcctataaa ctcagcatca acaatcacat
     3301 tattttcatc aacttcaaaa atcatttctc tacaacatac accacttgga ttaaatgtta
     3361 ctttcattaa aaagacctcc attaaatatt atttcttttt ttaacattaa taataatacc
     3421 aaatatatgc gtaataatgc aactataaag cataatacaa gctaatgaaa taatatatta
     3481 ttgtagtata tgtattgtag agaggagggg ttatatgaaa atctatattt ctaactactt
     3541 atcaaagagt attagcatag ttgattattc tacacttgaa ctagaaaaag aaatagtatt
     3601 agaagataat atatatccac accatttttg catagaaaaa gaaaaaaatt taatatacat
     3661 acctagttca agtaatggga tactctatgt cttagattta agtaatgata aaataattga
     3721 tactgtctct ataggaggaa gtcttagtca agttgtactt agcgataatg aattgtttgt
     3781 ggcaaatgaa gattctaata gtatatatgt actagataaa aacacattaa atccaatagg
     3841 aattattgga gttgataata tgccacatgg atttgacttt gatagagaaa gcaaaaagtt
     3901 atatgtaccg tgtataaact cgatagtatg tatagataca attaataaaa atatacaaaa
     3961 gaaaataaat atggacttta gagcttggca tatagaactt gataaacaaa agaaagaaat
     4021 gtatatatca acacttgatg gaaagcttat aataatagat gaagtaagca tggatataaa
     4081 aaaggtatta tatgaatttt tattaccagt agaaataaga tttaattata gtggcaaaaa
     4141 agtttatgtt gctgatttgg gttataataa tgtaagaatt ttagattata caacgagtag
     4201 atacattgga aatattgaaa taaatgggat tccccaagga ttagaaatat ctaaagatga
     4261 aaagttacta tttgtatcag atacacaaga aaattcagta aaagtttatg aaactgcaaa
     4321 taacaaatta ataaaagaaa tcaaggtagg aaaagagcct acaactataa tttgtttgta
     4381 ggctttaatg tatgtctctt ataagtaaat cgataacttc ggcatacata taagatgcat
     4441 tatttttcca gttattgcaa ataagttttg cttgtttctc tgatgaaaca tttagattta
     4501 aatctattaa gttagactga ttttctatga ctctaaggtt gacaataaat tcattatcac
     4561 tttgttttac aaaactagac tttacttgag tatctgctag tagattttct ttgttagaat
     4621 ctatatactt gtctattttt tcagtaacac tagtaggtat tcgattaaag aagtattcta
     4681 gactttctac gcctctttga gtaagagcat aatattctct gtcagagtct ttatatgtag
     4741 tcaaaaactt agattccata agctgagata aaagctgttg taatgaaaaa taattcatca
     4801 tctcagtctc taatactact tgagttattt gagaattagt taaatccatt ttaatttttt
     4861 ctaatatata taaaattaaa agtttgtgat aggctaattc ttcagacgag ttttcaaaca
     4921 taaatatcat cctccgtcaa actatttcta tatataatat attaaactaa tttgtacaaa
     4981 attaaaagaa caatttgtat tgtattatta atctttaata attcatataa aaacaatttt
     5041 ataaataaat atagaaaaac agggctgcta attagcagcc ctgttgatac ccaggtgtca
     5101 aaatttacta tttattttcc agccatttgt ctttcagcca tttcaactaa tttcttagtc
     5161 atatatccac caacatatcc attttctcta gctgttaagt tacctttgtc tatgttttca
     5221 tagttagata aacctatttc gttagctatt tctaatttca tttggtttaa agcagctttt
     5281 gcttctggaa ctactgttct gtttgaattt gacatagtaa atttcctcct caaataatta
     5341 gtttatgatg tctaacaaca cttttattta tgtgatgtta atactaattt ctccaaaata
     5401 aaaataatat atacagaaaa ataaaggtaa aaatgtaaaa aatataaaaa aattaaataa
     5461 atgagtattt tagagagttt aattaggtca atataggttt tattttttta gttattgtct
     5521 tataaattaa atattacagt acttttttga gctgaaaaaa aataaatttt aaatatagaa
     5581 gaaattattt caaaggggga atagtatgaa aagagaagtt aaaaggacta aagctttgat
     5641 atatagtgtt ttattttttt cattattatt agtgttaata tttgtttttg tttatggaat
     5701 aaaatacata tgttctaaat ctgacaattc aaaaatgcct atatataaag ttgatacaaa
     5761 tgagaaaaaa atagctttga gttttgatgt agcatgggga actgataata tggatgatat
     5821 tttaaaaatt ttagataaac ataatgtaaa agctaccttc tttttagttg gtagttgggt
     5881 ggatgataat gaagaaattg taaaagctat agatgaaaaa ggacatgaaa ttggaaatca
     5941 ttcaaatact catgccaatt tgaaggaaat atcaaaagaa gacataacaa aagagataga
     6001 aactacatca gaaaaaatat ataatataac aggaaaaagg actaatttat ttagacctcc
     6061 ttttggggac gtaaacaata aagctatgga catttgtagt gatttaggtt ataaagtaat
     6121 taagtgggat gtagattcta tagactggaa ggagcttgga ccaaatcatg taattgaaaa
     6181 agtaataaaa gaatctcaac ctggttcaat agttttattc catgctaata taaatgatgt
     6241 agataactat ttggatacta taataagtcg actaaaaaaa gatggataca gtctagtcaa
     6301 aatatctgat ttgttgtata aagataatta tgtagttgat tccaatggag tgcaaaaaac
     6361 taagaattaa taagtaataa atgtatattt taagtaaagt tgtgataaat tatatagtat
     6421 atgttagaaa agtgtcgata aaaagacggt gtctattttt tgacactttt tatacatgta
     6481 tgcaatatca ataattatag attaaattga ttaaaaaagt gtctaataat tgactttttt
     6541 ttgatgggtt taaaaattta gaagaattaa tgaatgctgt aagacagtat ttccaaaggg
     6601 tataagtttg tttaaaagtt ggcatagaaa ttgctttgtt aataagcgat atgttctata
     6661 gaaataaaca atatgttaag accttataat agaaaaatag cattaacatt atttgaacag
     6721 cttataaaaa aattaatata cttaaggggg cgtactagtt gaacaaacta tattctattt
     6781 tattaaaaca acacgttggt ggtccagata agccagtagt aagtgttgga gatgtagtaa
     6841 aaaaaggaac tctaatcgca gaaccaactg gattaggggc aaatatttat gccagtgtta
     6901 gtggaaaaat aagtgaaata aatgaccaag cgatagtaat tgaagctgat gaagtacaag
     6961 aagatacttt tgaaccatta aaaggagaag gtatacttga cttaattaaa gaagctggag
     7021 tagtaggaat gggtggagca ggattcccta ctcatgtaaa attaaacata gatttaaatg
     7081 gtggaacaat attagcaaat gcagctgaat gtgaaccttt attagctcat aatataaaag
     7141 aaatagaaga aagaccagaa atagtttatc aaggtataaa atatgctatg gaagttacta
     7201 atgctggaaa aggtatgcta gcaataaaaa gtaagcatcc aaaagcaata gaagctttca
     7261 aaaaagtaat aaagccagga gacaacatag aagttgcaga actagttgat atgtacccaa
     7321 tgggagaaga gagagcaata gtaagagacg ttctaggaaa attacttgag ccaactcaat
     7381 taccttcaga ggctaatgca gtagtaataa acgtagaaac actaactaga atagtagagg
     7441 ctgttgagca aaaaagacca gttatatcta aaaatataac tgtagttggt caattaaaca
     7501 gtggaaaaga atctatagtg tttgaagatg tgccaattgg aacaactgtc ggagaattaa
     7561 ttgaaagagc tggtggaata aaaggtgaat atggtgaaat aatcctagga ggacctttca
     7621 ctggtaaagc tactacttta gatgctccaa taactaaaac tagtggtggt ataatagtta
     7681 ctatgccatt tgttaatgaa aagagaaaaa tgggtctttt agtttgtgca tgtggaccta
     7741 atgaagaaag aatgagagat atagcaacta aaatgggagt tactgatata gtatcagttc
     7801 aaaaatgtaa acaagctcaa gaaattaaag gtgcattaaa atgtgaaaat ccaggacact
     7861 gtccaggaca agctcaaaaa tgtattgaat ttaagaaagc tggagcagaa gttatattaa
     7921 taggaaactg tactgactgt agtaacacag taatgggttc tgcaccaaaa ttaaaattag
     7981 gtgtatatca tattactgac catgtaatga gaacagttaa tcacccatta ataagaagaa
     8041 taaaaggtga cgtaagatta aaaataaaat aatagataaa tagaagttag acatctttag
     8101 aagtaaaact taaaagttac ttctaaggat gttttttaat gtattttgag ggcaatatat
     8161 atattgtaaa ataaatggaa aaaatagaca aaatatgcta gatattgtat aatgataata
     8221 atagctatta caattaatat tggacaaaaa taatgatact caagtatgtg gtttgtatta
     8281 atatgcatat tatattaagt caactatccg atagagtaaa aggtttttgt gatatttgaa
     8341 gatactctat agtatctttt ttattataaa aactaatttt tgtcttttat tagataagta
     8401 tctaggatac taatttatat tttattgagt tgttaaaaaa gtgcacttaa aaagttaaaa
     8461 aagaaaaatt aaaaaaccta tttatagaaa ctgtgaagta tatcttaaac ctgggcactt
     8521 aaaagatata tggagttagt agtgcaacct gctataaata taaataggag taacttttaa
     8581 ttgtcaaaaa tatataaaat aaatacataa ataagtaatt aatagaagat agatataaat
     8641 atatttttga aatgctccct gcattcataa cgagtgtaga gtggcagata tctgcccaaa
     8701 actcctttaa ttgctggaag ttcctaaagc taactaaacc acaacataat catgaaataa
     8761 ggataagtgt gatagagtgt gcatttaata agcacttgag gttacgaaag tagaaaaaat
     8821 tagttagata gcataaggtt aaatcctaag tgccttgaaa atggataatc agcaggtaag
     8881 cttcgaaaag aagaaacttc aacgactaga gagaaatctc gtacctacaa gtgtggggaa
     8941 atggggagta cctaatttat ggttaggtaa tgatatagtc tgtgcttaat agaaatatta
     9001 agaagttcat aggagaactg gttaggagta acgaccctaa ttgaacacac taaagatgta
     9061 ttattgaata agattcatat aatatttaat aattttaatt atattaagtg aatacaatat
     9121 atgtttttag tatcaggaga attttgaaaa caaaaattaa aaaatcaagt ataatcagtt
     9181 tagtaattgc ttttagtatg gtgtttactg catttacacc aatagtatct tatgctgacg
     9241 aagtgactag caatgataca atacttaatg gagaagaaca atcaaatggt cagacaccag
     9301 atgtagagaa accatcagac ggtcaggtac cagatgggga gaaaccatca gacagtcaag
     9361 taccagatgg ggagaaacca tcagacggtc aggtaccaga tggggagaaa ccatcagatg
     9421 gtcagatacc agatggggag aaaccatcag atggtcagat accagatgga gagaaaccat
     9481 cagatggtca gataccagat ggagagaaac catcagatgg tcagatacca gatggggaga
     9541 aaccatcaga tggtcagatg ccagatggag agaagacatc agatagtcag acgccagatg
     9601 tagagaaacc atcagatggt cagatgccag atggggagaa accattagat gagcaaactc
     9661 cagaagaaga aaaaccattg gaagaagaaa tagttataga ggaaatgagt ttaaaacaag
     9721 atattgataa aattcttgat atgactttat ctcaaataaa taaaatagta tataactttt
     9781 gggaagatga agaagatgta aaggcagatg aacaatcaga aattaaccaa gtttttactt
     9841 cagaagactc atttattagc ttatggtatg acaaaaaagc aaaagtaaaa aattcttgtt
     9901 tactaaaaga aatagatggt tcagatagac cttatcatga tttaagattt gatgatatta
     9961 caaagacttt aacttttgac tatgtaataa agggtcttgt aggagcaagc tcaaaagacg
    10021 acaaatatgt aatagaaggt gaagatggag agcaaactgc attttgttac aataatcatt
    10081 taagaccacc aagtagtaat ggaaagagtc catatttacc agcagaagat tttaatggtc
    10141 aagaaaacca aaatgaagaa gcagttaaat ctatactata tgcaggttct gaatttgatg
    10201 gatttggata taagcaacaa tttaatttag gtggagaaga aaatgaattg atgacttact
    10261 cagctactca aagtgcaatt tggattattc ttggacaaat ggatgaacaa gaaagactta
    10321 agcagtatca aggttctata aacctttgtg ataaacttat agaaagagca aaaactgaag
    10381 aggaaagagc tgagtatcag aagaagaaag aagtagctat aaatataaaa gcttatttag
    10441 aagcattgtt aaaagcaggt cgtgaagagt taaagccaaa tgatacagga aaacctagtt
    10501 tgagtaatgg tttaactaag attaactttg aaaaaaatga agatggtact tatgaaacag
    10561 aagctgtagc cttagtaggt tatagtggtg tagtaaagtt aagactacca gatggagtaa
    10621 ctgcttatga tggagatggt aatataatag gtacaggtga agtagaaatt tcaacacaac
    10681 agaaatttaa acttaagagt gttgtaaaac cagatgccaa agctaatatt tctgctgtat
    10741 cttatgatta tatttttcca aaagctattc aatattataa ggcagtgctt gatttaggac
    10801 aaaaagacca ttcatcttca ttaccatcta gtaagcaaaa tttattaagt tatacaatag
    10861 agaaaaaaaa tggtcaagaa gttaacttta atataggtct accaacagat gatgttgtaa
    10921 atccaccagt gccaccaaca gatgatacaa atggacataa gccaaaacca tcaccaccaa
    10981 tagatgatac agtaataaat ccaccagtac caccaatgga tgatacaata ataaatccac
    11041 cagtaccacc aacggaggat gcagtgttaa atccaccagt gccaccaacg gaggatgcag
    11101 tgctaaatcc accagtgcca ccaacagagg atacagtgtt aaatccacca gtaccatcag
    11161 taagtgatac agtggaaaaa actccagaac tatcaagaga tgatacaata ataaaatcac
    11221 caaaaacagg tgatgaaact cagctaatgt catatgtatt tattagtgtc attgcaattt
    11281 gtggattagc gtatcaatgt aaaataaaaa gaaattaatc tagattatta ttaagtttaa
    11341 aataaaaagt ctcaaattga tttataagtc attttggggc ttttttatgt aaatataata
    11401 tgcaaatata gtttgtttaa aaatatatat tttaattata ttcttaaatt tatgattgga
    11461 tacaggcatt cattaattgt ttgatgaata gaaaaatgtt ttgaaggtga aaatagtatt
    11521 gaaaaaataa tatactaagg gtataattca tttgatttca tttgtataaa tttaattata
    11581 aattttaaag tgattatata aattta
//
LOCUS       DAYATN010000122        11484 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.122, whole genome shotgun sequence.
ACCESSION   DAYATN010000122 DAYATN010000000
VERSION     DAYATN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.122"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..496)
                     /locus_tag="ACGI8A_002937"
     CDS             complement(<1..496)
                     /locus_tag="ACGI8A_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0180927.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INYYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKY"
     gene            complement(465..1181)
                     /locus_tag="ACGI8A_002938"
     CDS             complement(465..1181)
                     /locus_tag="ACGI8A_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0180928.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(1721..2521)
                     /locus_tag="ACGI8A_002939"
     CDS             complement(1721..2521)
                     /locus_tag="ACGI8A_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFH0180929.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(2835..3566)
                     /locus_tag="ACGI8A_002940"
     CDS             complement(2835..3566)
                     /locus_tag="ACGI8A_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HFH0180930.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEICAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(3553..4248)
                     /locus_tag="ACGI8A_002941"
     CDS             complement(3553..4248)
                     /locus_tag="ACGI8A_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0180931.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(4250..5041)
                     /locus_tag="ACGI8A_002942"
     CDS             complement(4250..5041)
                     /locus_tag="ACGI8A_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180932.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAIILSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILYTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(5051..5863)
                     /gene="modA"
                     /locus_tag="ACGI8A_002943"
     CDS             complement(5051..5863)
                     /gene="modA"
                     /locus_tag="ACGI8A_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0180933.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            complement(6497..6697)
                     /locus_tag="ACGI8A_002944"
     CDS             complement(6497..6697)
                     /locus_tag="ACGI8A_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFH0180934.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(6922..7098)
                     /locus_tag="ACGI8A_002945"
     CDS             complement(6922..7098)
                     /locus_tag="ACGI8A_002945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180935.1"
                     /translation="MFRDKMDKCTHMLTAYISNSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(7152..7241)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(7421..7621)
                     /locus_tag="ACGI8A_002946"
     CDS             complement(7421..7621)
                     /locus_tag="ACGI8A_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0180936.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(7623..7973)
                     /locus_tag="ACGI8A_002947"
     CDS             complement(7623..7973)
                     /locus_tag="ACGI8A_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HFH0180937.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            8135..8323
                     /locus_tag="ACGI8A_002948"
     CDS             8135..8323
                     /locus_tag="ACGI8A_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180938.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(8800..9000)
                     /locus_tag="ACGI8A_002949"
                     /pseudo
     CDS             complement(8800..9000)
                     /locus_tag="ACGI8A_002949"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9359..9847)
                     /locus_tag="ACGI8A_002950"
     CDS             complement(9359..9847)
                     /locus_tag="ACGI8A_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFH0180939.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            10651..11025
                     /locus_tag="ACGI8A_002951"
     CDS             10651..11025
                     /locus_tag="ACGI8A_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0180940.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            11130..11484
                     /locus_tag="ACGI8A_002952"
                     /pseudo
     CDS             11130..11484
                     /locus_tag="ACGI8A_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         4302 a         1492 c         1342 g         4348 t
ORIGIN      
        1 tatatttaat attttcatat attatataga ctatagatat aattataaaa ttgtagatta
       61 ttaaattagt gttgtaaaac attttattac ttgtaaatgc atgaaacctt aaggttaata
      121 gtatcaaagt taatataagt gtaagttcta tttttacctt agaaaaaagg cttatgacat
      181 tctctaagaa tttgacttta gtctgtttat tttttttata caatgtaaaa catagtatag
      241 tcaataaaaa acttgataat ataataccta tgcagacaaa ctctaattct acttttgaca
      301 aatgtacagg agtaccgaaa aaataaaaat aagggagagc aaactcttta taataataat
      361 taatattata agaaaagtta ttattcaaat cataaactgc ttcaaataat atagaaacta
      421 atagaaacag taataataat aaaataaatt ttgctttaga atcattaata attaagtttt
      481 tcaattttgt aaccaatgcc ccacaccacc tttaaatatt ttggttcttt tggattaatt
      541 tcaatctttt ctctaattct tctaatatga acagcaatag ttctctcaca actataagca
      601 tcctcattcc agacattttc ataaatctca tgagaagaga aaattttacc ttttcgtgac
      661 ataagaagtg taagtatttt atattcaagt ggtgtcattt taatttcttc accatctaaa
      721 gaaactattt ttgtatctgt atttagctct aggccaccac tttttagaat ataattagta
      781 tcatttgagt tatttgtatt actaaaatca cctaaattta tataccttct aagttgagat
      841 tttactcgag cagtaagttc tagtggatta aatggtttag ttatatagtc atcagcaccc
      901 atattaagac ctataatttt gtccatatct tctgattttg cagataagag aattattggt
      961 atattccttt tttctcttat tttaagtgta gttttaagcc catctaaatt gggcatcatt
     1021 acatccatta ttattaactt aatatcattt tcgattagta tgtccaatgc ttcaagtcca
     1081 tcatatgcct ttaaaacatt aaagttatca ggtctcaaat agaactctat agcatcaact
     1141 atctctctat catcatctac aaccaatata ttttgattca acttattaac ccccttgaaa
     1201 ttttattttt aaccaatttt tattttatac caaaaagagg tattagaaaa cagtaagccc
     1261 gttcattatg aaataaaaac tagtactttt aatatattgt caatgcatat agtaaattta
     1321 aaagtataga cgcccatatt aatctgaagg taaaagttta tgaaaatatc tagggtaatc
     1381 tatcacactc cttatgttat tttggtttat taatttttta ggtttaaaca tatattaaca
     1441 aatataaata acaaatgagt ttcataatta ttaagaattt ataacaaata tttattttaa
     1501 tgtagtttaa tataaatagg tagcttaatg tagatatgta taaagcattt aataaggctt
     1561 ataggtttta atgatgatat ctttatatat tttaagagat gatgaaatta gatatgggta
     1621 atagttgtat gagaaaaagt agtatagcac aaaaagtgct atatcacaaa atctcaaata
     1681 atttcctttt tataacttaa attaaaagaa taaatatttc ttataaatta ggtgtatgaa
     1741 gtatagcagg aacatcttta ttatttaata acttatcaaa tgatttatgg tcagacttat
     1801 agccaatacc ttctctatat tctaattcat caacaatttt gtattgttga caggcatttt
     1861 taaccttttc atagtcttca gtagaatgag ctttataaac tatcttatta ttaaaagtta
     1921 ttttatcagc aaatttaggt tttacaaaat atttagcatc ttttatttta ccagcattat
     1981 ataaattttg gataacagaa ccatttttta aatgttgtaa atggtcatct aacttataag
     2041 tatgtgctat atgagttaca tttttaaatt tttcttcaaa tgtaagagca attttttcca
     2101 tgtaattgaa gtcagtatta ccatttgtat ttgtttctgg taataatatt atattttccc
     2161 ttattacacc aaggctatta agagaagcta aaaattctct atttctatat tctgattttt
     2221 gtatatttgt taggttttca tattttttat atttatcaaa gacggatata ccacatccag
     2281 tagatactaa aaccacaaat acatgatttt ttcctctttc ttttatagca ttaacaatag
     2341 cactcccagc ccacaacact tcatcatctt gatgctgaac atagaagaca acataatctt
     2401 taaatttttt atgactataa ttcacatagc tactaaaagc ggttaaaatc aaacataaaa
     2461 ctagaataat agatagaatt ttatatttat ttatagtaaa ttttttattc ttaaatgtca
     2521 tttaatcatc tcctgaaaag ttgtcttagt tatattatca aacaaagtac taaaaattat
     2581 ttcttaaatg ctgaaaaata tagagtagat taatggaata aaattttttt attagtttaa
     2641 aataaagaaa acatttatgt ataagactaa tcaaaatatt aatgattgat aggaattgag
     2701 atttaaaggg attgtacatt tattcgggat tcattttaat agaattttac tttagtactt
     2761 agaataaaag taataacttt ttaaatttaa agagtacaca tatatatttg tgtactcctt
     2821 gatgatatga ttgcttattt actacaaaaa cagagtcttt ttaaacctaa aatatttgat
     2881 gcaccagcta tcgtagttcc ataatagtaa actggtttgt taagtgatag aaaatttata
     2941 attgagttat tacataatgt acttcctgtg caaatcacta aatcagccca agatattaca
     3001 tcttcataat ctcgtatacc atcttcaatc ttaacattat attggatagt atccacaaat
     3061 tcaggattta aatctaatac tcttgtttcg aaaaagtcct taatattatc aattatggct
     3121 ggttgatatc ctataattgc tacttttgga ttaccaaatt ccatttttat aaactcaggg
     3181 aatttttttg cacatatctc tggctcgtta tttttacaat gaatggttct atccgtcttg
     3241 cctaagtatt tcataactgc atttaatgat gctataaaaa gacttctatc atgaggattg
     3301 tcatgtaaat ctaatgataa tatgtctttt aaagaacctt caaatgtaga tggagtagaa
     3361 gtaaatgctt gtcctttagc tcctttaaag tcagcttcaa gcattatttc tttaccaact
     3421 ataattggga aatctttacg cttagtattt ccaattgctt cttttgaaga tagagatttg
     3481 gacacaatac ttatcatatc catatttaaa ttatatttat caacttgtac taatagataa
     3541 tctctcaaaa tatcaaatat attcatagaa aaaagattct cctttatttt ttatttcttc
     3601 atttaaatca aaaatatgat ttatttttcc attgttcatt attcctatct tatgagcaaa
     3661 aaacattgct tcagaaatat catgagtaac atgaatcact gttggtttgt attttatgta
     3721 gacatccttc aaaattttgc ttatatgttt ttttgtaata taatccaatg aactcatagg
     3781 ttcatctaaa agtaatatat caggttcagc tattaaggca cgtgcaaaag caactcgttg
     3841 cttttcacct ccacttaaat ttttaggata cctatttttt atatcttcta tctgcattaa
     3901 tttagataag tggttagcct taagaatctg ttcatctata ggtatttttc tcaatttaag
     3961 tggaaacctt atattatctt caacattcat atttggaaat aattcaaaat tttgatatac
     4021 aaatcctata tttctatttt ctggaggtaa gtttgatatt aaattttctt tgtatgatac
     4081 atttccttta gcagatggat atcttcctgc taatgtttct agaaatagag ttttacctga
     4141 cccactagct cctaataaaa caaagtattc atgattatta atatttaaac tattttcttg
     4201 taatttaaaa ttaggtaaat caagagataa attttttatt tttaacatat taattcttat
     4261 caacctctct attaaaaaat atctcaaaaa gcatcaagca tgtaagtgag ataaatatca
     4321 aaattacagc tattccgatt gcaatgtcta tatcaccagt agacatattt aaatatatag
     4381 atgtaggaat aatttcagtt ttcatacgtg tagaacctgc tatcatagct actgcaccaa
     4441 attcaccaag tgctcttgac caagtcatta cagttgcaga tatgattcca gtttttagat
     4501 ttggaagaat tataaatcta aatgcttccc aactattaca tccaagggta cgtgcaacaa
     4561 atatcatctt ttcattactt tcatttagtg aagtgtaaag tattttaata gtaagtggaa
     4621 gatttacaaa tacttgtgct aaaactatac cttgctttgt aaatataaaa tcaagcttaa
     4681 aaatcttata aatggaatct ccaatcccca ttcttccaaa caaaagtaaa agagcaattc
     4741 ctgatactaa gtgtggaaga gacataggga gatttattat ctgtataatc agttttttaa
     4801 atggtaattt agtaatatgt aaaaaatatg ctactggaat agctaataaa aggcatacta
     4861 tagtagatat taaagatgtt ttaatactaa gtattattgc atactgtact tccactgatt
     4921 ttaagcatat aggaacataa cttatccctt taatgattaa cataagaagt ggtgatacaa
     4981 taaacaaaaa gactatagca aaaaatccag taagtagagt agtaaaaaat ttatttttca
     5041 agtttaatca ctacttaaca ggttcaaaac catatttttt aaatatctgt ttagcttcgt
     5101 ctgactctat ataatttata aatttattag ctaaatcttt tttatcacta tagtttacga
     5161 ttcctgctga tataagttgg tctatatttt tatcttgggc aatttctata acttctacat
     5221 cttcattgcc aaagaccgaa tcttttgtgg ctattgtagc atcagcttta cttgatgtta
     5281 actgtacaat catttcattt acagttgcag ctgtagagac aacattatca tttatagatt
     5341 ttaaattatt tttttcaagt attttttgtg tagtcttacc tatagcatta gactctttgt
     5401 ctcccaaaat aagtttaact ccatctttat ttaagtcttc caatttttga atattagctg
     5461 gatttccttt aggtacaact attgcaggtg tatgatgtgc aaccgtttta tgttcaccta
     5521 caagttcttt ttcaaaggct tttgaataaa ttggttcaga accaacaata aatacgtccc
     5581 ctttgtgact tgtctcaatt tgagctatta gctgtgcaga acctgcatag ctatattcaa
     5641 ctttgacatt attttctttc tcaaattttt ttgctatttc atccattggt ttctttaaac
     5701 ctgcccctga atagacaaaa agagtctctt tttcttttga atcatcagct gttgtcactt
     5761 tagtattaga ttcatcatta gctttgtttt ctttagaatt acatgcagta atacttaatg
     5821 aaataatcat agctaaagct agtactgaaa gttttttaaa cataagatat tcccccttag
     5881 taaattaatg taaattcttt tttagtatat catactaaga aaacgatgtc taagtttttg
     5941 ttatgtttta taatgttttg ttatgttttg ttatgtatag ttaagaaaaa atctatttat
     6001 aatgttattt ctataggtag ttattttatt tttaattttt attaatactt taaagtattt
     6061 taaatttaaa aacttaaatc aaattaactt attgtagatt ttttactttt taactaattt
     6121 tatatttatt atatttcagt gaaaaaatag aacattgaaa catatagata tgttttaatt
     6181 gctttttatt aacaaattac taaaaatgtt atatcagaat tgaatacaaa agaaagagta
     6241 tgtatttatg agatagttga aaattttgat gtgccacaac taaaattatt atattactat
     6301 atatttaatt aaaagagtac ttgtaggttt aagtacatac tttataaata tatttttgtt
     6361 agatttattt tataaaaagt atttagacta agtattattt ttaagtattt tcttgttgaa
     6421 gttatttttt ccttttttac aaaattcgta aataaaaaaa agactcttaa aagagtcttt
     6481 ccgaaaaatc tatatattat aatatagtta tattttctgc ttgaggacct ctagcgcctt
     6541 taactatatc aaagcttact ttttgacctt cttctaatga tttaaatcct gaagtttgta
     6601 tagctgagaa atgagcaaat acgtcatctc cactttccat tgatataaat ccaaatcctt
     6661 tttcattatt aaaccatttt actattccat tattcattaa aaatactcct attaacctat
     6721 atcaagatat acttgattta cttgtgtatt accacaaata ttattgtatc atagattaac
     6781 ataaatgtct atttaattat aatttttagt taacattttt atttattctt aatatgaaaa
     6841 taaatttaaa cattttatat taccaatata ttaatgtaaa tgtagataaa actaaaaagg
     6901 gttttctaga aaaaaatctg tctaattata cttacttacc aataccatca cttgatgcaa
     6961 gcaagattcc acaacttttt ctccgtactc taatataaaa tcatctaact gtgtatctat
     7021 aaaatcacaa taatcgtatg aattactaat ataagcagtt aacatatgtg tacatttatc
     7081 cattttatct ctaaacataa tgtatttatc cccctaaaaa actttcatga aaaacaaaaa
     7141 gaaaatatct ttttggcaac tggctgacat aacttatttt ataagcgtta gtccctatag
     7201 ctttgcgtca ctaaatttct ctagttttgc cgatttagtt ttattctaca gattaataat
     7261 acaatagaat tagttatcat tcaacatatc tagttgaata attaataaat ttatatatga
     7321 actttaaatt atttgaaaaa ataaaaagcc cctctgtcag gagtgactta aaaattgaat
     7381 ttaagtttgt atttaaaaca catttttaat aatgcaaaaa ctaatcaaaa ataaacaatt
     7441 catcaatagg tgtatctact atttttgata tatctattgc taatttcaaa gaaggattat
     7501 actttcctgc ttctaaacgc ataatggttt ctcttctaac tcctactttt tctgctaatt
     7561 gttcttgagt taattctttt aattgtctat atttttttag gttacaagtg aatttaggca
     7621 tattattttt cctctaaaat ataaagtttt atagcataaa catttaaagt gattgcatat
     7681 gtaattgata gtaaaacaca cattaattct atattcctag acactatagt aatacaccat
     7741 aaaatgaata attctataat agcaagctgt cctgtaaatg ccatagctgc tttttcatct
     7801 tgtacatacc tttcatctgc tttatcacca tttatttttg ctataataaa gtttgagaaa
     7861 aaagcaaaaa atccaatata tgtaaggtca gcaacattgc cagatgaaaa atatctaaga
     7921 cttaaaaatc caaggaaacc acaaaaaccc ataaaaaatt ttttctgaat cattttttat
     7981 cctccttagt aattttaaaa gagatatatt tatatcttta tgatacaaat atatctcttt
     8041 tatattcttt tgtcaataga aaatcagtat tatacaatat ttatactgca ccataattgt
     8101 tagagcaaaa atctaatgct tgagatgttt atgtatgact aaatatgatt ttaatttcaa
     8161 attaaaagta gctaaatttt atttgaatag agaaggaaga gatattttaa tagtaaaaca
     8221 atatggagtt tatgaccatt ctcaagttga aagatgggtt aatatattag gagagagtga
     8281 actaaaaata aaaaaatttt ctatactata tggttcaagt tagatgttat aaactatatg
     8341 atgataacta aatatagtgt acaataaatc gctaagtatt ttggaataaa caatagcaca
     8401 gttatagcaa catgaaaata aaaattttta catagtggtg ttgatgcctt ttctaaaatg
     8461 aaaggaagac cataatgaat aaaaacaaaa acactaaaga aaaaataaat taacaagaga
     8521 acaagaatta gaatatgaaa ataaattttt aaaagatgag cttgagtttt taaaaaagat
     8581 gtgtgtctca gggaagccac attaaaaaac agtggtatca ttattaatca aaagaaagtt
     8641 agatgtatta tcaaaaaatt agaacttaaa tgcatagcat tttttcataa atcacataaa
     8701 tataactcct ataaaggtat aattggaaaa gtaaaaaata ataaaacaaa tagaagattt
     8761 aaaacatcaa tagcatatca gaaaataaca aatgatatgc tagaatttat aaaatatatc
     8821 taatgataaa aatggaaaat caattataaa aaaggcatat ctcgattcat tttttgttat
     8881 atagtcaaat aagaatacta tagatgaaag atacgatttt gcatatgtat taagtaagta
     8941 ttctgatgtt tttggttgat acacatgcaa taaagaagtt gtgtttgcag ataaattagc
     9001 aaaactatag tataagttgc aacaataaag aaaatgaaaa ttatcatact aaagcataag
     9061 gcatgttaca attttattag ataggacatt aacatacact aaagtacttt ttattataaa
     9121 gagtacttat ttttttgaaa ttcattaatg tataaactat caagaatatt actcaacata
     9181 atttaaaaac aacttgaata ggtattttta aattatgtga tataataaaa aatagaagtg
     9241 tatgccaata cacttctgta gttataacta agcattctat tcttgaggag tgcttttttc
     9301 atttttataa ccaactcttt ttactttaac aataattatc agacctatat atttattttt
     9361 aattgtctga tttattagat aaaaaagtta ttatcatatt tttatcattt ggtattttta
     9421 ttgaactacc ataatcttct tctaatttat ctagaccact tttacttatt tctccatcta
     9481 taattatgtt taattctaag tattcttcac taccattaga tgacactaaa gtgtacgaac
     9541 cattataagg catgcctaaa ttttctattt tatgtgccaa agggtcttca tcaccaatac
     9601 cagagtttac tgttattttt ccatcttttt tagtccacca tattatgttt ccttcagtat
     9661 attttgattc atctgcatag tagctaagag acgcactttt tattgcactt atatcagctt
     9721 caagtttagc tatctttgct ttttctatat ttttaaataa tgctggaagt gctactattg
     9781 ccaatattcc tattatagca attactacta ataattccac tagagtaaat ccttttttat
     9841 ttttcattta tttcccctcc atataaaatt aataatatat aaatttttat tttgttaatt
     9901 tctactttct atattttacc atatatagac aaaaaaatca ctttctatga caaatatact
     9961 attattttac aatttatgga aatgatttaa aatgacattt cttgttagaa aagatataag
    10021 aaatggatta aaataaagaa aactacagtc atatcaatgt caataatatt atttaaaagc
    10081 agatgaaaat aaaaagagga ctcagaaaat aatgtctatt taattatact tacttaccaa
    10141 taccataact tgatataaac aagattatac tacatttttt tatgctctag tataaaatca
    10201 tctaactgtg tatctataaa atcaaaataa ttatatgaac tatcagtata ataagtcaaa
    10261 atatgtatac atgaatacat attttaaaag atatatatta ttacatacct aaaaaacttt
    10321 cataaaaaca aaaataaaat atgcttttag taacttgtta acataactta taatttattt
    10381 tataagcatt agcctatata gctttgtgct actaaatctc tttgattcta acgatttagt
    10441 gctattctac aactaaaata atacaataga gctatttatt gtttggtagc attgtttgta
    10501 aattaaacaa atttatacat atgaatatta ttttacattc aaaaaaacag actaaatatg
    10561 tagaatattt ggtaaaactt acctctagtt tttgtcttaa aaagctccta ttattaactt
    10621 aagttagaag aattaaggaa gtgacataaa ttgaaagaag aaatattaat agaaaaatta
    10681 ttaagagcag aattaatagt aatggagtat ttatggaaaa aagacactct aatgcctaaa
    10741 aaagaaattg taaaagaaat aaaacagatt tatggatggc ataaaagtac aataaaaata
    10801 ctactaaaaa ggctagttgc taaaaggtat ttggctagat atattataaa ttctcaatct
    10861 cattataaaa taatagatag taaagaatat tatactttta agaaaaaggt actaaaatct
    10921 agtaaaagca taaaaataat gcgttcactt accactacac ataaaaatgt aagtaaagag
    10981 aagttagacc tattagatga gtattataga aacttaaaag aatgatatag taaaaatcga
    11041 atgtaattat ttaccttaat tcttttgttt gtaaaacttt attgatacag aatttaacat
    11101 atacataaat tggtaaagga atgtatacta tgaaaattag taaattacca gaagcggaat
    11161 taaaagttat gagatatatt tgggaatcag aaaaggtatt aatatcaaga gaaatcacca
    11221 agtctatgga acaaaaatat gaatggaaag atacaactat atttacagta ttaaaaagat
    11281 tactaaagaa gagagttttt agaagcggag aaaatagata agtgtacaca ctataatgta
    11341 ttagtaaaaa aaaataatat cagagatttt aaacaaaaga atttttaaag aatacacata
    11401 aaaattcaat attaagttta atgtcagcac tatgtagtaa ggatgatgaa gtagatggtg
    11461 atattttaaa tttattagaa gaat
//
LOCUS       DAYATN010000123        11374 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.123, whole genome shotgun sequence.
ACCESSION   DAYATN010000123 DAYATN010000000
VERSION     DAYATN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.123"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            59..370
                     /locus_tag="ACGI8A_002953"
     CDS             59..370
                     /locus_tag="ACGI8A_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFH0180941.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      463..555
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            655..1449
                     /gene="thiD"
                     /locus_tag="ACGI8A_002954"
     CDS             655..1449
                     /gene="thiD"
                     /locus_tag="ACGI8A_002954"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFH0180942.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1473..2261
                     /gene="thiM"
                     /locus_tag="ACGI8A_002955"
     CDS             1473..2261
                     /gene="thiM"
                     /locus_tag="ACGI8A_002955"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFH0180943.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDALSKNSNIEFLN"
     gene            2284..2916
                     /gene="thiE"
                     /locus_tag="ACGI8A_002956"
     CDS             2284..2916
                     /gene="thiE"
                     /locus_tag="ACGI8A_002956"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFH0180944.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3055..3357
                     /locus_tag="ACGI8A_002957"
     CDS             3055..3357
                     /locus_tag="ACGI8A_002957"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0180945.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3423..4172)
                     /locus_tag="ACGI8A_002958"
     CDS             complement(3423..4172)
                     /locus_tag="ACGI8A_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180946.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4166..5128)
                     /locus_tag="ACGI8A_002959"
     CDS             complement(4166..5128)
                     /locus_tag="ACGI8A_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0180947.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5132..5590)
                     /locus_tag="ACGI8A_002960"
     CDS             complement(5132..5590)
                     /locus_tag="ACGI8A_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0180948.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5946..6302
                     /locus_tag="ACGI8A_002961"
     CDS             5946..6302
                     /locus_tag="ACGI8A_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0180949.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6374..7090
                     /locus_tag="ACGI8A_002962"
     CDS             6374..7090
                     /locus_tag="ACGI8A_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180950.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7083..7907
                     /locus_tag="ACGI8A_002963"
     CDS             7083..7907
                     /locus_tag="ACGI8A_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180951.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            7919..9118
                     /locus_tag="ACGI8A_002964"
     CDS             7919..9118
                     /locus_tag="ACGI8A_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180952.1"
                     /translation="MKLKSKISIFIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            9127..9939
                     /locus_tag="ACGI8A_002965"
     CDS             9127..9939
                     /locus_tag="ACGI8A_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180953.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            9950..11218
                     /locus_tag="ACGI8A_002966"
     CDS             9950..11218
                     /locus_tag="ACGI8A_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180954.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIIDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT         4552 a         1180 c         1803 g         3839 t
ORIGIN      
        1 atttttttta attctataat ataatattta tatatttaat gttaggatgt gatttaaggt
       61 gtattttaca tatattataa gatgtaaaga tgattctcta tacactggat ataccagtaa
      121 tatagttaga agaatgaatg aacataaatt aggaattaat tcaaaataca caagagcaaa
      181 gggatttgaa aaactagaag tttattttgt gaccaacaca aaaagcaatg ctatgaagtt
      241 agaatactat ataaaaaaat taactagaaa taaaaaattg tctataataa aaaacccaag
      301 tatacttata aatttaattg ataataaaga agattatatt attggtaagg aaatagaaca
      361 attaacataa atatttatag taacattata atgggtgcat atggtgtaaa aacttgacaa
      421 taaatactat tgtaatataa tgaagttata aatatagtaa attaaaatag ctaggggagc
      481 caagagctga gaggtatacc gacccttaca cctgatctgg ataataccag cgtaggaaag
      541 cttagtaaaa attcaagtat gtattatttg tgatatatgc tatttctaag ctatacctat
      601 tgtaggtata gcttttttaa ttaagttatt taaattaaac tttaggaggc aacaatgagt
      661 aattataaaa taccaacact tacaatagcc ggctctgatt catctggagg agctgggatt
      721 caggctgatt tgaaaacgtt tagtgcaatt ggtacttatg gtatgagtgt aataacagct
      781 ataacagcac agaatactaa aggagttttt gctgtagagg atttaaataa gaaaataata
      841 aaaaaacaaa tagaggcagt ctttgaagat atacctccaa gagctgtaaa aataggaatg
      901 gtttcaagtc ctgagattat acttgaaata gttgagaatc taaagaaata caatccaaaa
      961 tatttggttg tggacccagt aatgatatct aaaagtggat attatctatt aaagccagag
     1021 gcaaaagaaa acttaattaa gtatttgata ccacttgcat acataattac accaaatata
     1081 ccagaggcag aagaaataac aggaataaaa atacataatg tagatgatat gaagagagta
     1141 ggagaagaaa tactacaact tggacctaaa tttgtactta tgaaaggagg tcatttagat
     1201 ggagaagcag tggatatttt agttggtaaa aatatattta aagtttataa aagtgaaaga
     1261 attgataaaa aaaatacaca tggaacaggg tgcacattat cttctgcaat aacatcttat
     1321 ctagcattag gatatgaaat aacagaagca gtaaatctaa gtaaaatcta tataacagaa
     1381 gcaataaaaa gaagttttga tataggtcat ggagtaggac cagtacatca tttttataaa
     1441 tttgagtaat aaaaaaatat tggaggtaga atatgtataa tttaattaaa gatgtaaaaa
     1501 aattaaatcc attggtaatt cattatacta ataatgtaac tataaatgat tgtgctaatg
     1561 taactttagc tgtaggagca agtcctctta tgagtttttc atatgaggaa gtagaagaaa
     1621 tggtaagtgt agcaaactct gtagtaataa atataggaac tatgaactca aatatgttgg
     1681 atttattcct attagctgga aaagcagcaa ataagtacaa taaacctgtt gttttggacc
     1741 cagttggagt atttgcaagc aaagcgagag cagaacttac aagtagactt ttaaatgaag
     1801 tcaaatttag tgttgttaaa gggaatgtat cagaaataaa gtttataggt ggctttaatg
     1861 taaggggtaa aggtgtagat tcatttgatg aagaagaaga ttctactgag ataataagaa
     1921 agattgctga aaagcttgaa tgtgtagttg tagcaactgg aaaaatagat ataataacta
     1981 atggtaaagg tacatataaa ataaacaatg gaactgataa attaaaagga attacaggaa
     2041 ctggatgtat gactgctagc ttgattgcaa gttttatggc tgttactgag aatatattag
     2101 aagcagcaac aatgggtgtt cttacaatgt ctttaagtgg ggaattagct aatttaaata
     2161 atccaccaat aggtacattt aaagaaaatc ttatgaatgc tatttatcaa atggatatag
     2221 atgctttaag taaaaattct aatattgaat ttttaaatta ataaagagta agggagactt
     2281 aaaatgattg ataaagaatc tttaaaaaaa tgtttaaagt tgtatttagt aactgactct
     2341 gaaatgctta aaggtagaga cttttataaa tgtcttgaag atgcaatatc ttctggaata
     2401 acaactgtac aacttagaga aaaaaatgct tctggaagag agtttttaag aaaagctatg
     2461 aaacttagag aaataactaa aagatatggt gttaagttta taataaatga tagagtggat
     2521 atagcactta tttgtgatgc agatggagta catgtaggac agagtgacat tgatgttaga
     2581 gaggttagaa agcttatagg aaacaataaa attctaggag tatcagcaag aactttagaa
     2641 gaagctattt gtgcaaaaaa tgatggcgca gactatttag gtgtagggtc tatattcaca
     2701 acatctacta aattagatgc aaaatcagct tcgtttgaaa ctgttaaaga aattaaagaa
     2761 aaagtagaca tgccatttgt actaattgga ggtataaatt tagacaatat agataaactt
     2821 aaatgtttag aaagtgacgg gtatgctata atatcagcta tattaaaagc tgaagatatc
     2881 tctaaagaag tggaaaagtg gacattaaaa atataaaatt ggctctggta gtgaaaaaaa
     2941 tggaaaaatt tgatataatc taagagtaaa atttaagcaa ttataaatga taaacttttc
     3001 tacatatact ttttaatcaa gatatatgta gatttggtta aaaaggagct ataaatgatt
     3061 aggatattgt tagtgtgtgt tggaggcatg tcttctaccc tattggtaaa taaaatggag
     3121 aaggatgcaa aaaaaagaaa tatagactgt aaaatatggg cagttggaga aggagatata
     3181 aaatcggagc ttgataactt tgatatttta cttcttggac cacaacttag atttatgctt
     3241 gatgacgtaa agtctatagt tggagataga gctccagtat ctataataga tatggtaaac
     3301 tatggcactt gtaatggtca tgctgtgtta aattcggttt tagaaatatt aaaataaata
     3361 ataaaagtgg attctatata atagagtcca tttttattat ttgttattaa taggcttaaa
     3421 acttagctta ccatttcttt ttttaatctc cacatagcaa gtgaaaaact tgaacagcac
     3481 aatattaaaa atagtaggat aacaggcatt atatctccaa taagaatagt ctcaccaaat
     3541 attaagctac gtaaagcgtt aataacatgg gtaaatggat tgagcatcac tgctactttt
     3601 aatcctccag ataaactttc tatagggaaa agagctgaac ttaaaaagaa aataggaagt
     3661 acaattgcag tcataattgt ttcataaact atttcatttg gcaataataa acttatagaa
     3721 taagctagac ttgataaaaa gaaggctgtc ataaatatca gtactatcat aagtaggatt
     3781 ccaccaatgc cagattctat ccttactgag aagaatatac ttactataca caaaatagtt
     3841 acttctatga aagaaactaa gatagcttct aataattgac caaggacgat tgaatacctg
     3901 ctgataggtg caattaagac tctatagaaa cttccactgt tcttcattat aaagtttata
     3961 ataccaccac tactacaaga agaaaacaca accagtacaa tgatacctgg tagtataaaa
     4021 gcagtataat tattgatatt tatattattc atagtttggt ttgcaataga actataaagc
     4081 acaagccaaa gaaaaggttg taatatagta actacaaatg agattggatt tttaaaacgc
     4141 cacttcatac tacgccataa gatatttaac attcccattc ctcctttaag ctttttttag
     4201 aacttgtaag tgctaaaaat acatcttcga gacttggctc tacaatttct attgcatcaa
     4261 attctatatc atgttctaaa agccatttat ttattaaagt aaaagcagtt ctactatcgt
     4321 ttacacttat aaaaattgaa ttttctcgta cgctcataaa ctttactaaa ccagtacttt
     4381 taattgaatc tttatatttt tttatatctt cagtattatg aaaagttata cgtagtatat
     4441 tttgtctaat gtaactacgt aaacttgatg gtgttccttg tgctaaatcc tttccattct
     4501 tcataataca aatattatca ctcaattgtt cagcttcttc aagataatga gtagttagaa
     4561 atatagttgt gccatactca tctctaattt ttaataacat atcccacata gattttcttg
     4621 aatcgacatc cataccaaca gttggttcat ctaaaaatag gattttagga gatgatacca
     4681 tattcatagc tatgtccaat ctacgtttta caccccctga atatgatgaa gtaggatatt
     4741 ttaagtaact agataagtca aacttatcaa ttaaagagtc tattctttgt tttgcaatct
     4801 gtggttctac tttatacatt ttactttgaa ataccatatt ttccataagg gataaatgtt
     4861 catcaataga gatttgctgt gctacacaag caatttgagt acgtatccaa gaaggattat
     4921 ctactaaatc ttttccaaac attgttacat ttccagatgt aggtttgtaa aaggtagtca
     4981 aaatatttat aagagaagat ttaccagcac cattaggacc aagtaaagaa aaaatttctc
     5041 cagcatttac attaaagctt agaccattta aagctttcac accattttta tattctttta
     5101 ctaaattgtc tatttcaatc gctaacatat attattcctc ctctttaagt ggaaatatta
     5161 tctctgtaat atagttatct gggtttcctt ttatcaacat acctggtcct ttaataaata
     5221 cttctctcca aggagtttgt atttttatat tattttcatg aatatattta aatatcattt
     5281 catacgtttt tggcaaatta ctataagagc ctgtgtgagt aaaaaataaa gcttttgttt
     5341 taggaaattc ttttgtagtt atacccatac gatttggagt ttcagcagtt ggaacacata
     5401 attccatttc tgcaacacca gttttttggt caacatcata gtagcaaaag aatggtgcac
     5461 cgttagactt tccttttata gctttaaata catttggaaa atactttgtt gcctcagtca
     5521 taggtccatt ataacgcatg gtagccactg tgactgattc gatatttttg atttcaattt
     5581 tgcaattcat ttgtttacct cctaatgtgt gttcatattc ttactagatt tttaagcatt
     5641 aatacatata agattttgat tagttgaaaa catataatct tatctcgttt gcttgttttc
     5701 aagtctaaca taacataaaa tatatatcct ctcatttgat gctccatttt gtaaagttat
     5761 tatttaagct ttactttaga ttttttagca aattaaaata aagcaattta taaaataagt
     5821 atttacaaat taagtgtatt aatcgtataa tacacttaat aggatactag ataaagtctg
     5881 aaataaaggc gtattttttt aaagtaagtg tactatcgta ttaatacact agaataggag
     5941 gaatcatgaa ttttgaatta gataatacaa ctcccattta tttgcaaata gtaaagtata
     6001 taaagagaca aatagtaaca ggagagttga aaccaggaga gacaatacca tcaagaagag
     6061 aaatggcact taatctaaaa gtaaacttaa atacagttca aagagcatat aaggagatgg
     6121 gtgatatgaa tataataaat acttttaaaa attatcaaag cagtgtaaca gttgatgaaa
     6181 atatattaaa aaatttaaaa ttagagctta taaatgaatc actatctgtt tttatagaag
     6241 atatgaaagc aataaatgtt agcaaagaag aagttttaaa aataatagaa gataagtatt
     6301 agttttttaa caaatagaaa acttagttta gaaagtttat ttataagcat attggatgtg
     6361 gaggatagta aatatgatag aaataaagaa tgtcagcaaa acatataaaa ggatgcaagg
     6421 gcttaaaata agaaagatag aagctttaaa aaatgtaagt tttaatatag aaaagggaaa
     6481 aataactgca ttacttggta taaatggtgt tggtaaatca actatgttaa aagcaatagc
     6541 aggacttata aacattgata gtggagaaat tcgtatagat ggagaaaaaa taaatgaaaa
     6601 ggtatacaat aaattggctt ttgttccaga tgtacaatct cactttagca atacaacaat
     6661 aaaagaaaca tttgaattta tggaaatttt ttattcaaag tggaataaag aaaaatcaaa
     6721 agaaatgatg gacatattta aattggatga agatgaaata attgataact tgtcaaaagg
     6781 aaacattgca agagtaaaat taatacttgg attttgccaa gacccagagt acatacttct
     6841 tgatgaacca tttacaggaa ttgatttgtt taagagggaa gagtttatag gagtaatagc
     6901 gcaatatatg gaggaaaatc aagccattat tataactact catgagatag tagagataga
     6961 aagtttagtt gatgaggttg taattcttga tgaagggcag attataactt catttaatgc
     7021 agaagaatta agagaaagag aaggtaaatc tatattagat aagatgaggg aggtatataa
     7081 gaatgaataa acttctatat ctaataaatg tagatttgag aagaagcaat aagttttata
     7141 tagcttatat atcatttttt tcattaatta cacttagttt aaatttattc gaaattaata
     7201 aatttaaaaa ttctaaattt attatgaact tagcaataga agattttgga ggtatatttt
     7261 atggactagg gatacttagt aatcttggtt ttatccaaag tatattgttc tttgggatat
     7321 tgggactttt tatatactat atttttatgt ggaaaagaga gttcttttca aataatcaaa
     7381 gtatttatac acttatgatg ttacctcaaa ataagtttaa gatttgtata tctaaatcaa
     7441 tagccttgat aacaatgata tatggttttt tgataagtca gacagcaaca ttatttatat
     7501 gtaaatatat atttaatttc atttttagaa atatgcctat aatcaatatg aactttgcca
     7561 aagatttata ttatttaaac ttgaagagta taccaattga ttttatctct tttatggcta
     7621 tttatgtatt tttactactt atagctattt ctattgtaaa ttgctgtata ttatttatct
     7681 tttcctatat aaataaattt tatgtaattt taatcacttt gctgattata gcaggcttat
     7741 atttttcaac aatatatata aatataatca ctttatttag aaaatattat aatcttatag
     7801 gatttcaatt taacaattta gtagcagggt tattaagttt tttaattata atgtttattc
     7861 taaatggaat atcttatttg ctaattaaaa agaaagtatc tctttaagga ggggtggtat
     7921 gaaattaaaa tctaaaatat ctatatttat tatatttttg actttagcaa tatattttag
     7981 tataggttta ttaaataatg aagataattt aaagataaaa actataaaag gtgactctaa
     8041 tgaattagac catataaaca taaattttgg atatgctttg aatggatata taaattcata
     8101 ttatacaata aatggaagca acaaaaccaa aaagactgaa ttaataaaac tacaaaagga
     8161 tagtaagaac acaaataatt taattgatgt atctagtggt tcagatttaa ttgctcatgt
     8221 tggatttgaa atgaatgagg agtcaaaaga tataaacttt gtgatagatg agaaaaaaga
     8281 agatttagaa aaaggtgtat ttccatcaga aggtagtgat aaagaaattg aatatgaaaa
     8341 attagatatg aataatttta caaggacaag atataaagta gataaaagta cagatgatta
     8401 ttatgcaaag atactagcat caaaaagata taaaaaatac ttatatgtat ctgtacttta
     8461 tgctacaagt gattcatcag aaaataacaa agaatattca agtactatag atataataaa
     8521 aattaatcca gaaaataaaa aaatcaagac tataaaaagt ataaatttaa gtaattattt
     8581 ttccgaaaaa taccaaaaca tagagaacat aggtgtaagt tatgctagca aatatgagga
     8641 taagatgtat attttagtaa atgtactaaa tacaaacata ggtgataata gtaattctaa
     8701 atttcattta cttgagtata atcttgaaga aaataaggaa agcataatgg aattaaaaac
     8761 tgatagcaaa atggctttag caaaagcaaa gttagaagct aataaattat atatggtaat
     8821 gtcgaatgat tatgataata gttcaattac agagttaaat ttacttacct ataatatatt
     8881 agataaaagc ttagatgaag ataaatatgt tttagataat agagaaaaaa gaggaattag
     8941 aagtattgaa attgattcaa ataaaatcgt tgcatataca tcaaggctaa actctatatt
     9001 atcttgggat gataaattta tgctatatat aataaataaa aattctaagg aaattgtata
     9061 tgaaggagaa ttacaattta acagttcaaa tagtatgtta gaatttgaaa taaagtaggt
     9121 gagttgatga aagatttaat aagattattc gaatttgaat taaaaagaaa tataaagaat
     9181 tatatattta taataatttg ttgttgttct tttgtaattt taaacatagt aaaaaattta
     9241 aatgactata attacataat agaaaatgca gttaaaagtg aacagttaac aaaaatcgga
     9301 aatacgatag aaacggtaaa tgttacaggt ttctcaagct ttagaaatat aatgggttct
     9361 acagaatcgt ggtttatgtt tggtataatt gcatgtattt gctatgcttt ttttatttgg
     9421 tatagagact ttaatggtag aagtaaatct atatatacac ttataatgct acctaaaaat
     9481 agaatcaata tttatatatc taaattatta aatatattat ttctagtgta tagctataca
     9541 gtagtactta caatatcttt gtttattgca agtaagttgt tgcctagaca tatgcttggt
     9601 aatgtcacaa actatggttt tgtacaagaa acaatatatg agttaaaaat gttacttcca
     9661 tatagctttg aaatactatt tgtagagtat atatttttat tgataggatt tgtatctgta
     9721 gtatttacat caattcttat aaataaatct atttataaag taagcacttt aataagtttt
     9781 ctatttttag ttatagaaat tttagtgttt ataatttcta tagaatttat tataccttac
     9841 gaatacagtg atgtatttac tgttctatat tcaagtataa acatatttgt atgtagtctt
     9901 atatctaata aacttttgaa gcaaaagatt gacttttagg agggatatta tgaaagcaaa
     9961 aaatatagga aaatatacaa tatgttttat aatagtatgt tttttaatga ttactatgac
    10021 cttaatattg agttatttta ataaggataa actatctgta aaaactttaa aaggcaatgt
    10081 aaaagatata gggagagtaa gtgtagtata tacacctaat ataagcaaat tttcttatga
    10141 ggaggtaatt ataagtaaaa atggagtaag aaataataca aacccaaaat caagaattag
    10201 aaatgattat ttaaatattg acaaaaaata cagtgacttt gttaatgata aaggaaaaga
    10261 atttatatat aaagatgatt tgaatatagg agaagcgtat acctctgtta agtctgattt
    10321 agtacaagga atgaatgctt taaatgattg tataaaggtt tcaaataaaa atttaaaaag
    10381 tggggtagaa gaaaattttg atattaattt aaataagtct gattttaaag aaaatagttg
    10441 gaaaacctta tttaacaaaa tatataaagg taatttatat atggtaatta gtgaaactaa
    10501 tatttcaggt tctaaagtca ataatatttg tatattaaag atagatataa aaaataagtc
    10561 ttataaatta ttaaatagat ttaatttagg attacagaat ggagaacaac aaaatatcaa
    10621 tgaaattaaa ttttctattg gaagtaaatt atacttagaa atagtaaata ataaaagtaa
    10681 aaatggatat attgaaggac atgatagtta ctctaacttt attatatatg atatagaaga
    10741 taacactttt aatataagta ataaatttat ggataatcta aacaaagaaa agaatgcaaa
    10801 tccaaaggag attggcttat taagtaaaac taagctagat tatatggtaa aaaacaataa
    10861 attgaatctt atagttacgg atatgagtaa ttccataact aatcttgttt atgatattaa
    10921 ttctaatgag attaaattag aagattatga gatacttgat ttaaaagtag attataatga
    10981 agcatcatct ggagttagga aagttaagct actggatgat aagatatatt ctatttcagg
    11041 tattaagaac agtatatcag gagaattaaa aggatttaaa gggattattg atgtagtcgg
    11101 aactaagcca gtaaaatttc aagtatttga tatgaaactt aaaaagaata tttatgaagc
    11161 tgagctaata aatggagata taagtttaca agaaaagtta ttttttgttg caaattaagt
    11221 gttagaatta attttagaac tttaaacttt tatatgtatc acagataagt ataagtaagg
    11281 gagtgtctca aaataaactt tttagctcat gagacacttt ttttatatga aatcaaatat
    11341 attttcattg tttagattta ttttgataat gaaa
//
LOCUS       DAYATN010000124        11294 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.124, whole genome shotgun sequence.
ACCESSION   DAYATN010000124 DAYATN010000000
VERSION     DAYATN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.124"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            114..395
                     /locus_tag="ACGI8A_002967"
     CDS             114..395
                     /locus_tag="ACGI8A_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180955.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            659..913
                     /locus_tag="ACGI8A_002968"
     CDS             659..913
                     /locus_tag="ACGI8A_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180956.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            958..1281
                     /locus_tag="ACGI8A_002969"
     CDS             958..1281
                     /locus_tag="ACGI8A_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HFH0180957.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(1840..2034)
                     /locus_tag="ACGI8A_002970"
     CDS             complement(1840..2034)
                     /locus_tag="ACGI8A_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0180958.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(2408..4027)
                     /locus_tag="ACGI8A_002971"
     CDS             complement(2408..4027)
                     /locus_tag="ACGI8A_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFH0180959.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            complement(4336..4554)
                     /locus_tag="ACGI8A_002972"
     CDS             complement(4336..4554)
                     /locus_tag="ACGI8A_002972"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0180960.1"
                     /translation="MFFSLFKKKSGYTFTNFLSKVRVEKSKKYILKKDLSILDVAVLI
                     GFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            4847..5038
                     /locus_tag="ACGI8A_002973"
     CDS             4847..5038
                     /locus_tag="ACGI8A_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180961.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(5127..5939)
                     /locus_tag="ACGI8A_002974"
     CDS             complement(5127..5939)
                     /locus_tag="ACGI8A_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HFH0180962.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            6361..7512
                     /locus_tag="ACGI8A_002975"
     CDS             6361..7512
                     /locus_tag="ACGI8A_002975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0180963.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVISDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            7630..8268
                     /locus_tag="ACGI8A_002976"
     CDS             7630..8268
                     /locus_tag="ACGI8A_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HFH0180964.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            8269..9291
                     /locus_tag="ACGI8A_002977"
     CDS             8269..9291
                     /locus_tag="ACGI8A_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFH0180965.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            9281..9901
                     /locus_tag="ACGI8A_002978"
     CDS             9281..9901
                     /locus_tag="ACGI8A_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180966.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(10025..10711)
                     /locus_tag="ACGI8A_002979"
     CDS             complement(10025..10711)
                     /locus_tag="ACGI8A_002979"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFH0180967.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQNKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(10725..>11294)
                     /locus_tag="ACGI8A_002980"
     CDS             complement(10725..>11294)
                     /locus_tag="ACGI8A_002980"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0180968.1"
                     /translation="KLKREDIYMNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLS
                     QIERGLANPSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIY
                     EILIPDSSGNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKG
                     DSVKVPLGTKHKWQNDSNSESKVIFAVIL"
BASE COUNT         4449 a         1264 c         1601 g         3980 t
ORIGIN      
        1 ataaaacaag attagaaaat aatagtgtta ctttcataat aaatacatat aaagatattt
       61 ttaagaataa aatttattat taaataaaaa ataaaggaga gtgatttaat agcatggcat
      121 caaaacaaat accaagaaac aatttagaaa agtttttaag aataagagtc ttatcacata
      181 caggcgataa aatacatata aagttacctg taaattttgc aaagagaatg attgagaata
      241 atgctttgga tttatttaat ggaaaagatg atgtagtaga tggtaaaaaa gttactgaaa
      301 tattagttaa ggcatttgac tatgatttaa ctggagaagt agttcacttg gagagaaaaa
      361 atggagacat tattaaagtt tcaattgtac aatagaatta ttttatatga attttatttt
      421 tgaatagtta taaataatta gaaatttgta aaaaagtata atatattatt agtaatggta
      481 cagaatacac atttataatt tatagatgtg tattttttgt ttaaaaaatt taaatatata
      541 aaactataaa ggttacaatt ttggaaatag atgtaaatat atgttgtatt gtataataag
      601 agtaatataa aatctagtaa aaataatcaa ataaatttag cttatgagag ggataactat
      661 ggaaaataag gattttaaat taacaatatg tatatttgct tttgtaattg gagtagtagt
      721 attattctgt tgtataggtt taggtgaatt tagtgcagag catattattc aacaaaatgg
      781 tggaatcatg gatactgacc aatataatat ttatctagac caatctattt ctcaatatag
      841 aaattttggt tctatattag ctctgttagg aggaataggg atattgtttg ataaatatat
      901 cagaaaatac tagagattat gttttgtcta aaataaattc ttatgaggag ataaaaaatg
      961 gcaacagaaa tatttactgt aaaaattaca cccttagagg cacaaaaaat gataaaagaa
     1021 aatcttgatg cagatttagt ttttagtgat tcttataatt taggagatga taaatttata
     1081 ctaattacaa cttttgagaa gtattatgca agaaataata gtgatgcagg attaatagta
     1141 gtttatgaaa ataccacagg aaaaacagtt attaaagcta cttcaacagg cagtgcaatt
     1201 ggaattttaa aaattgattg gggtgcaggt ggaaatttac ttaaaagagt aaaaaacata
     1261 cttgctgatt atattattta attattttat ctaactgata atatgtcaag aaaatctcgt
     1321 aaaagaggtt aagaacattt ttataatata tagataaata agaatttgaa ggtaaagttt
     1381 ttaaggtata aagaaaaata atataataaa acgaattggc tttataattc tttataaggc
     1441 aaagagcaca tttctttata taattgttgg atattaaata atatgtaaat acaccattta
     1501 catataaaag tttaattaat atgttaagag aaaatgaaat ggaaattact aaaaaatatt
     1561 tttaaattgg ataatggttg atattatgtt tatacaaaaa tcatgtagaa ttttatatat
     1621 tttaaatatg atagtttaaa attatgaaag aatgataaat tccagtttgt agggtaattt
     1681 tgataaataa tattaataag tatgagtaaa aagtaggagc atattaatat aatgtcccta
     1741 cttttttatt attaaaattg tttataatat caacactaca ttaaaacaca acctaaattt
     1801 gaaaaacaaa tagtttagaa atatgtactt ttttatctat tatattcttt taaatcgaat
     1861 tttttcatct gaacataaag tctcaaatct atatgcgtct gtcatatcat aaaaaccaaa
     1921 aaatataaag ccatcacgag aagtaaattc atattgtata ttttcaattt tcaacatgtt
     1981 ttctacttcg cttatttttt cgctatatat aatttctacc atataattta tcattgtaga
     2041 atccctccta ttaatatgaa aatatccaaa tatataataa catatttaga tgatataata
     2101 gtatccaaat aaaaaataat attattacat tattgaatga agaattaaaa tgaagataaa
     2161 taaaacaaat attggttata attgattaaa aaatagttat gtatacttgt gactttattt
     2221 atgggcagtt caagttttat gaaagaagtc atatatatat ggaatattct aggcagaaac
     2281 tgtttaataa taagagaata ctataaaaat ttgagtatta cataaaaaat aagaagccat
     2341 aaatgatata agcattatct atagcttctt acttctcatt gattaagatt atatataaaa
     2401 ataatcacta tattttttgt ttaagtttta gtacttgttt tactatattt gttgagtggt
     2461 cagaaattct ttctagatta gtaagtaagt caagataaag tacaccagca tcaattgtac
     2521 aacttccatt atttagtcta atcatatgtg aatttctaaa agttttttct aaataattta
     2581 cttcattttc aacttctaaa actttatttg ctaactcaaa atctttaaca tctaaaacta
     2641 ttaaggcatc cttaaaatta gtaattgtct tattgtatat ttcgtatatt tctcttatcg
     2701 cactatcaga aaaactaatg ttcatttcct taacactcat ggccaattca gctatatttt
     2761 ctgcatggtc agagactctt tcaatgtcat ttacagtatt aaaaagcaaa tctgttctaa
     2821 agcgttcatc atctctaaga ggttctttag aaagttttaa caaatagttc aatatatctt
     2881 tctgtaactt attgacaact ttttctgact caaaagcttt attaatatct tcattgctct
     2941 tatttaaaaa tcctgcaatt gaatgttcca aagagtttgt agctttatga cccattctta
     3001 caacttcttg tacagtatta ccaagtgcta tagatggagt gctaaacatc ctttcatcta
     3061 agtacttagt attatttact atttctgatt cagtttcttt aataggtata agtctatttg
     3121 ctaatttaac tatgtattta gaaaatggta ataatattat aacatttgta agattaaata
     3181 aagtatgtgc atttgctatt tgtctagctg tatcagttgg gtcaaaatga gtaactatgg
     3241 ctgatatagg tttatttaaa acaagcataa atattattgt accaattaca ttaaaactta
     3301 aatgcattac agctgcacgt cgagcatttc tacttgcacc aacacttgat aaaagtgaag
     3361 taacacaagt acctatatta tctccataaa gaataggtaa agcagatgat agaggaagta
     3421 ttccttgaga agctagtgct attaacatac ccatagatgc acttgaactt tgaacaatac
     3481 cagttatggc aaatcctgcc aatataccta atacaggatt tttactaaaa tataaaagtg
     3541 catcagtgaa tgctttatat tcagcaagtg gagctactgc atctttcata aactccattc
     3601 ctgtaaataa tataccgaaa cctataagta tctcagataa atgctttgtt ttttgatttg
     3661 atgtaaataa atagaataaa atacctatac ctaaagcaat tggtgcaatt ccttcaagat
     3721 taaatgaaac taattgagca gtaactgtag tacctatatt tgcccccatt attacaccaa
     3781 tagcttgact tagattcatt atacctgcat ttacaaatcc tacaaccata acagtagtag
     3841 cacttgaact ttgtataata cctgtaacga ctgtacctac cagtacaccc ataacaacat
     3901 tacttgttaa gagttcaata atttttttta atttgtctcc tgctgatttt tgtaaacctt
     3961 ctcccattaa actcattccg taaagaaaaa gtccaagtcc accgataagg ctaattacta
     4021 tattcacgat atttcctccc ttaagaaatt aataattttt ttatctgaat tataaacttt
     4081 attaactaaa atttattgtt tccataatat agaataacaa attttgtcaa taaaaatatt
     4141 aatgttttgt taagtatacg ttaagagaat gttaaatttt taagttgttg ttaattttag
     4201 aaaatttatg atatatagca atttaaatac ttgaacttag atttttagta ttttatgtac
     4261 aatcaattta acaaagaatt aactataatt gtaaaaaata tgtaaaaaaa taacatcctt
     4321 ttagaatgat attttttagt tataaatatt tttatattca attggtgtaa agttattaaa
     4381 ctttttaaaa attatactat agtaattttg actattgaat ccaattagta cagcaacgtc
     4441 taaaatagac aagtcttttt ttaatatata ctttttgctt ttttcaactc taactttact
     4501 taaaaaatta gtaaaagtat atccactttt tttcttaaat aaagaaaaaa acaactttta
     4561 ttaagttgta attcattaca tatattactt actgttatat cttctgagta atttttgtga
     4621 atataatcta tagttttctt taagttttct ttacataaaa ctaagagttg gtctatgttt
     4681 cattttatca aggcatattt attaaaaaat caatctctat ttgttaaaat aatttatgaa
     4741 tgatgttaga aactgtaata tattggatat aatataataa acagataaac tctgtattaa
     4801 gaggatatat agtttatctt aattataggg gaagtgagga tatattatgg aaagattaat
     4861 ggaactcata gaaagaaaat ttttaaagga tgtagaactt ttagaattag aagacataga
     4921 aaatgtaata gaatgcaaaa tgacacagcc atataatgct cattataatg aatatttagt
     4981 aaaagtaaaa aatggagatg aagtagaaga atattttgtg tatttaaaac atagataaac
     5041 ataaaagagg tttatattta tttgtatgat tataaaataa atacaagcct ctttttttga
     5101 taaatataaa attataaaat ttttatttat ttaatttttt caacaacatt agtcaatatt
     5161 gaatctaaat catcactaat ttctgtaact ccattatgag taatatctat aatttcttta
     5221 gtcataggaa gttctcctaa aagctctaag tctaaatcat ctaaaaactt ttcagtagat
     5281 tctccttcaa ataacttgat ttttttagaa caatcaggac attggatata actcatattt
     5341 tcaataactc caaggacatt tatgttcatt tttttagcca tattaacagc ttttgataca
     5401 atcatagata ccaaatcttg aggtactgaa accataacta ttccacttat aggaatagat
     5461 tgcataactg ttaaggcaac atctccagta ccaggaggca tatctattaa taaatagtct
     5521 aattcttccc aaagaacatc agtataaaat tgctttacaa cccctccaag taaaggtcct
     5581 ctccaaacaa caggttcatt ttcatcatct atcataaggt ttatagacat tacttttatg
     5641 ttatttgagt tttctacagg gtaaatataa gagccatcag aataagcttt tacatttttt
     5701 acacccataa gtctaggaat acttggtcca gttatatctg agtctaatat accaacctta
     5761 tatcccattt tgtttagctt attagcaagt aaagcagtta cagttgattt tccaactcca
     5821 cccttcccac tcataacacc aattattttt ccaaatttat tgttagggtt attttcaata
     5881 gaacaatttg attgactatt acaatttccc tttgatggac atgaattaca atctgccata
     5941 tctattacct ccaaacttta atatttatta gattatttgt aacctacata tactatttta
     6001 gtacaattga accataaagt cattatttaa aagcaactta gaagattttc tattgattta
     6061 ttcatagact atagcaaaaa gaatacaata aaaaaattat aacattcttg aaaaagtgat
     6121 tatttcaatt tagttataga acttagtcat ataaatatat agaaaatatt ttaatatgtt
     6181 atacataacg ggttatttta taagtttaac tataaattag gtttatattt tataaaaatt
     6241 tgctaaaaaa aacaattaaa aattgtaaag aaaaaagata taattagaac tgtagtaata
     6301 aatgttttat tatagataat agagttttaa gtatcttaaa taatatttgg aggaatatga
     6361 atggaattaa gaccaagaag tgcaaaatta gaagaatttg ataaagtaat agaattaata
     6421 aactatgtat ttagaatatc aagaaatcat aaaccaacta tgatggaaga atttcctctt
     6481 ttactaagta aaaataatat agaaaatatg attataatat cagaagatga taaagtgatt
     6541 tctgatgtaa actatctaat tcaggatgta tctattcaag gaaacagatt gaaggttgca
     6601 gctataggtg gagtatgtac tcatccagat tatgaaaaga gaggctattc atctaagata
     6661 ttggataaag tagaagaaaa aatgttttat gatggtgtag atattgtaat tatatcagga
     6721 acaagaagtc tatacagtag aagaaattgt agtttggtaa agagttttta taaatataca
     6781 ataaaacctg aagatgtaaa aatagcatat gaaatagttg aatttgatga aactaatttt
     6841 gaaaaagata atgatttaga taaaatgatt gaactatata atcaaaatag tactagattt
     6901 ataagaacga gagatgagtt tcaaaaacta ttgcatgcag caactattgc ctggggacca
     6961 attggttaca agaaggtatt tataaaggaa aataataata taataggata tttgataata
     7021 agaacaataa aaaaagaaga ttctactgta ggagaagttg ctgaaatagg gttaaactct
     7081 gtaaatgttg aaaatatatt gaaatatgtt gcaaataaat ttggattaga atatttaaat
     7141 tataaagtac atgtgaaaaa tcttaaagac cagttgaaat gtaatgggac aaaatctttg
     7201 gattatcaac aaggaactat gaaaataata aattttacta agttgtgtga tagtttaaga
     7261 agttatttta gtcaatatgt agattttgaa ttactaaaat acatggaatt taaacaagtt
     7321 gaaaataaat atattattaa atacaaagaa gaagaacttg taatagaaaa tttggataag
     7381 cttaataaac tgttttttga aaaaaatgag gaacagtaca atgaatttaa acatctaaaa
     7441 aatatatatg aatttgctac aaaggcattt cctgtagatt ttccatggac tgcaaattta
     7501 aattatcaat agttttagat gatattaaat aaaattgatt gatacctaga gttagtcctt
     7561 gttaaataag agaaattatt ggatattgaa aaagcaattt tgtaagtaat tagaaaggat
     7621 gagtattata tggacatact tgatgatata gcagaatgta tattaaatat gggtgtagat
     7681 aatatagaga atttagtaaa aattgctata gataaaaata ttgatgtgga agatatttat
     7741 gaatatggtt taaacaaagg tatgatagga gctttagata agtttgagaa taaggaatac
     7801 tatctttcag aagttatagt atgcacagat gcattaaata aaggtattaa tcttttgaag
     7861 ggaactggaa aagtaaagaa aaaatcaaag ggagtaattc tgatgtctgt agtagaagga
     7921 gatactcatg agataggaaa aaatattgta aaagtaatgg tagaagccac tggatataag
     7981 gttattgact taggtgtaaa tagaaaaagt gaagatataa tagaagaagc tataaaaaac
     8041 aatgtagata taataggctt atcttcaatg atgacaacta caatggaaaa tatgaaatcg
     8101 gttatagacg aacttaatat gattgagata gataaaagac caaaagtaat aattggtgga
     8161 ggacctgtaa gtatggagtt tgcagaagaa ataggagcag atggatacag ttcaaatgct
     8221 ccaaaagcag ttaaattaat aaataaactt ataggaggtg aagtttaaat gttttcagat
     8281 atgactttac ttcagtatat aaatagtgaa gatagaggtt tttttctgcc tgatatgggt
     8341 acaaatggat tgttcttaat aaataaaaag gcatatgaag tatatgatag tgtagatttg
     8401 caattagaat tagcaaagac tatggacaag tattttgaat cagattttat atactcattc
     8461 tgtgatggga ttacattttg tgaaactctg ggtttagaga cattaagacc agattatgat
     8521 tttccaagtg tattgacaca tactataact gacagagata aattaagaaa attagatgtt
     8581 ccagaccctt atacgagtgg acgtatgcca ctcaatatag agagcttatc agttatatca
     8641 aaaagtatta aaaaaccact atatgaatcg atacaaggtc catttacact tgcaggtcaa
     8701 ttagctggag ctactcaact tttaagatgt ataataactg ataaaaaatt tgtagaagaa
     8761 ttattggaat ttacaactga attggtaaga agatatgctg tagctgcaaa taaagcagga
     8821 gcaaaatata tttcaatagc agagccaaca tcagtaaccc taagtaaaga tagatttgat
     8881 gaatttatag taaaaaattt aaataaaatc tacaatgaac ttgattgttg gaaaggtatg
     8941 catatatgtg gagacaccag agaattatta gataatatgc taagttgtaa tatagatgca
     9001 gtaagtttag accaaatttt agactatgaa gaaattgctc caatgattcc tagagatata
     9061 gttttgattg gtaatttaga cccaataaaa cttcttggac gttctaagcc agataagatt
     9121 agaagagaga cgctaaaatt gttaaagaag atgagaagat atgataactt tttatgtgat
     9181 tttggctgta actgcttaaa taccactcca gtagaaaatt tacaagcagc tataaaggca
     9241 ggaagaatta gttataaaga attagatagg attgatattg atgaattata atagtaataa
     9301 aaataaatat cttttaaagg atataaattt agatgatatt tttatagata agaatattgt
     9361 gctaaagttt ttggggtatg gaaatagaaa agctccagat acaattagta aaatcgttga
     9421 aagagaaata agaaatataa atgatatttt agatatcaag atatatataa gtgaagttga
     9481 tattaataaa acacctaaag aatgtaaaaa agcatttgct gtactatata caatagggga
     9541 taaaattgat attaagatga atgactatat ggaaaattgc aatatgatgg cagggctagc
     9601 attggataaa ataggtatag tatgtttaga ttatattaat gagaaaataa aaatgtattt
     9661 aaaaggaaag tatgatagtc tcaaaatatc gcatgaaata tatccaggag ataaagattt
     9721 tgaagtagag agacaaaagg atatttataa ctatgtaaaa aataaatata atagtataga
     9781 aatagaaatt aatgattatc atcaactatc ccccataaaa agtgttgcaa tgcttatttt
     9841 aatgggggat gaagaaaatt tagaatcaag atgtagtaag tgtcctagaa aatgctttta
     9901 gttaattttc aagactaaga tttgttttgt gtataaattg caataatttt tttggtcatt
     9961 ttatgtataa actaaaaaaa ttattgcaaa gtaatattct atttaatttg aacagactta
    10021 caaactatag tagtttcttt aattcaataa gtgtatttat ttcataagtt ggtatatgat
    10081 tagtataatt tatactgtta ttggaattta accaacaagt atctattcct gaatttattc
    10141 cacctaagac atcagaggtt aagctatccc caattattaa ggcagaagat ttatcatgac
    10201 aattaaactt tttgagagcg tatttaaata tttcaggatt tggttttgat attttcaact
    10261 cttcagatac aaccattcca tcaatatatc cttttatttt tgaattttca agtcttttat
    10321 tttgaactgc agaaattcca tttgttatta taacaatagt atactttttt ttaagatatt
    10381 gtaatatatc gattgcacct ggaattaaaa atgtacattc acctaaaaaa tttaagtaca
    10441 ttttgctaag agtttcagaa tcaacatcca aagatatttt atttgcaaaa agttcaaatc
    10501 taagtaattt aagttcattc aaagttataa tatttttttc aaggtcgagc caaagcttat
    10561 cacttatatt tctatatgac tctatataat tctcaaaatt aaattctaaa tcaaactcag
    10621 atagaagttt tttaaaagca tgtgattcac ttttcttaaa atcaaataaa gtatcatctg
    10681 catcaaaaaa tataaattta tatgtcttca tggtttttac tcctttacaa gatgactgca
    10741 aaaattactt tactttcact atttgagtca ttttgccatt tatgttttgt tcctaaagga
    10801 acttttacac tatctccttt ggacaaagta aattcatcat catccataaa aagtttaact
    10861 tctccctcca aaacatatgc aatttcatct ccattatgag taatcctatc aacacaagaa
    10921 gaagtgtttg gagctaaatc cataattgca aattctaaat taccactaga gtctggaatt
    10981 aaaatctcat atataacttc tttgcttcca ggtaaaataa ctttttttct attatcatgt
    11041 cttactatca agtttttttt atctacttca gatacaaaaa atgtaaataa aggaacattt
    11101 agagaagagg ctatagattt taatgaattt acagatggat tagcaagacc tcgttctatt
    11161 tggctcaata atgatggtgt aacatcagct agttttgcta aatctcgaat acttaagttt
    11221 tgtttttttc gaacttcagc tattttttcc cctaaattta aatcattcat ataaatatcc
    11281 tctcttttta attt
//
LOCUS       DAYATN010000125        11220 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.125, whole genome shotgun sequence.
ACCESSION   DAYATN010000125 DAYATN010000000
VERSION     DAYATN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.125"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1334
                     /locus_tag="ACGI8A_002981"
     CDS             <1..1334
                     /locus_tag="ACGI8A_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFH0180969.1"
                     /translation="TYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKIIL
                     ILGYSIISQMIIWLSDPINILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGLNF
                     LFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSNKI
                     WIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANIGV
                     ILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLEIE
                     DIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDNHL
                     GIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPINR
                     KQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(1670..2239)
                     /locus_tag="ACGI8A_002982"
     CDS             complement(1670..2239)
                     /locus_tag="ACGI8A_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HFH0180970.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVEGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            2642..3214
                     /locus_tag="ACGI8A_002983"
     CDS             2642..3214
                     /locus_tag="ACGI8A_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HFH0180971.1"
                     /translation="MEYIINPCSGIKIKITRGQKIEIVDIEGGQVVDFFAEMKDDSTE
                     FISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPCCRPEMYDFFYH
                     NGENHPNCFDNINRTLGTSHQIIHPINLFMYTKVNTDGSIEVCEPLSKANDKIILEAL
                     ENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(3893..4795)
                     /locus_tag="ACGI8A_002984"
     CDS             complement(3893..4795)
                     /locus_tag="ACGI8A_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFH0180972.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            complement(4922..5041)
                     /locus_tag="ACGI8A_002985"
                     /pseudo
     CDS             complement(4922..5041)
                     /locus_tag="ACGI8A_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(5584..6177)
                     /locus_tag="ACGI8A_002986"
     CDS             complement(5584..6177)
                     /locus_tag="ACGI8A_002986"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFH0180973.1"
                     /translation="MVGAVAGLIVLLYRILLGQAGTWLSQILKFVEGSTIKIIAWFAV
                     LAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKIKQVWWRVLPTKFIGGFLSLMGGLA
                     LGREGPSIQLGAMAGKGISRALDRGKTEEKFLLTCGASAGLSAAFHAPLAGVMFSLEE
                     VHKNFSVSVLISVMTASLTADYISANFLGMESVFQFD"
     gene            complement(6409..6945)
                     /locus_tag="ACGI8A_002987"
     CDS             complement(6409..6945)
                     /locus_tag="ACGI8A_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HFH0180974.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(7018..7431)
                     /locus_tag="ACGI8A_002988"
     CDS             complement(7018..7431)
                     /locus_tag="ACGI8A_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFH0180975.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            7897..8451
                     /locus_tag="ACGI8A_002989"
     CDS             7897..8451
                     /locus_tag="ACGI8A_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFH0180976.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISSMNGTISDMQGSISSMDGTINSMQDSITDMQVEIKEVNSRLDN
                     MEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVKDTSDIK
                     NDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(9059..9262)
                     /locus_tag="ACGI8A_002990"
     CDS             complement(9059..9262)
                     /locus_tag="ACGI8A_002990"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0180977.1"
                     /translation="MCLDAVIYMLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDI
                     VFSCIVFHHCTNVQNIFCIFWKW"
     gene            complement(9464..9997)
                     /locus_tag="ACGI8A_002991"
     CDS             complement(9464..9997)
                     /locus_tag="ACGI8A_002991"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFH0180978.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            10314..10445
                     /locus_tag="ACGI8A_002992"
     CDS             10314..10445
                     /locus_tag="ACGI8A_002992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180979.1"
                     /translation="MGKVRIGCKKEKYFYVRNPVKSLVDRITENEQFAIYHFTHKYN"
     gene            10764..10952
                     /locus_tag="ACGI8A_002993"
     CDS             10764..10952
                     /locus_tag="ACGI8A_002993"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0180980.1"
                     /translation="MPYKYFTEFCLLKATQFLKGTNELISDIAIKVVFHQIIYFSKCF
                     KEKTGYSPKVYRNIKKEI"
BASE COUNT         4011 a         1886 c         1353 g         3970 t
ORIGIN      
        1 gcacttattc tttaatctct acaagcataa attttattct aatattaatc aatattttca
       61 tagccttttt tctttataag ttgacttcct attttataga ctttaggaat cattggattt
      121 tcaaaatcat attgattttg ggctactcta ttattagtca aatgataata tggctttccg
      181 accccataaa tattttgttt acactattcg gatattttat actacttata atatgtagca
      241 aaagcaaatt aattcctata atatcagttg tcatgatact ttatcctatt ataattgggc
      301 ttaattttct ttttataaac aaccctatat atgaggaact tatacactca aaagagtata
      361 ttttaatact ttcaatagtc cagataattt ctgctctaat taaattatta ttttggtttt
      421 taatacatat tttcctaaag aatagattgg tcaatataaa acaatactta tcaaataaaa
      481 tatggatttt tattgatatg gtatgtatat cttcattttt atcaatatta attgctatta
      541 ttctgccacc tactaattta atccctatgg gtagtaataa gaatattatc ttaggcaact
      601 taccttatgg cacttatcta atcatcttgt cagcaattct ggcaaatatc ggtgtgatac
      661 ttcttttaca gccattgatt gaaaatgtaa agatgaagat agaaaaacaa gcaaatcacc
      721 taaaagaaaa atactataac ttactagaaa atcaacaaat tcaaatacgc aaaatgcgac
      781 atgatatgaa taatcacttt caaatgattg aatcgtattt agaaattgaa gatattgctg
      841 gagctaaaaa gtattttaat cagttaaaaa taggtatagg tactcttagt ggaaaacaat
      901 tttgcaaaaa ccaagctctt aactcaattt taaatcttcg tcatacaagt ttagaagaaa
      961 atggtgttga tgtgcacttt aatatagata tagacaatca cttgggtata gaacctatag
     1021 atttatgtac tattttttca aattctttag ataacgctat agaagcatct ttgaaaattc
     1081 ctgatttgtc aaatagaaaa gtggtattaa aggcacgttg tgagaaggag tattttagct
     1141 atttattgac aaacaacaag gtaaatccta tcaatagaaa acaagaacta tttatttcag
     1201 ataaaaaaga tagtaattct cacggatatg gtattgagaa tattagagaa attgttaata
     1261 agtacaatgg aaaattggat atttcttata ctgagagtga attttcttta tttctttata
     1321 ttagattatt ttagtctaaa attagattat cttatataat acaaattaga ttgtttgata
     1381 ctatgtaaaa ttataataac ttataattaa atatttacac atatattcat taacttaaaa
     1441 tttaatcaaa tgtgatattt tatttacctt agtaaaaatg tgtgtgtctc aaaataaact
     1501 ttttagttga tgaggcactc ttttttacat gaaatcaaat atatttttat cctttcaaca
     1561 atgaaaaatg gatttatctc tccattttga aacaagccct ttttctatta attactttct
     1621 taaaaaatat aatacgatat ataagataaa tatctaattg tacctatttc taattatatc
     1681 tattagaata caacaccttt gccttttcag taaactccct aacaaaatct agtttactat
     1741 ctgtataagc atcacgatta tgctcatact tcttccaaag gtccaactta agtttttcat
     1801 actcctttgc aacatctggg tggtcaatta agtaatctct aaaaaacaac tcatcattat
     1861 cacctacata ccttatatgc aaatgaaaaa ctcgttcttc aaacccctct actgtatacc
     1921 ctttattaag tgaaagccta ctactgctct cagacataca aatatatcca ttcgactcaa
     1981 tcaaatcttt aatatctttc atattataat caggaattat ctcaatcagt atgtcaacaa
     2041 tgggcttggc ataaatcgta ctcacagaag tacttccaat atgacttatt ctaacaattt
     2101 tttgcattgg caatattcca gttagtctat attgttcttc tttaaaccaa tttttccaac
     2161 attctttgtg agtagtcaaa aatataggaa acaagttcca taactcctct aaagtcatat
     2221 cttccaactt ctttttcatt agttacctcc ttttaaattt gtactttata ttttttcttt
     2281 ttcaaattta cttagttaat atttaacttt attttttatc aaatcatttt tctaattatg
     2341 taaatttttc taaatttagt ttttaatgta ttataacaga tttttaccct tcttacactt
     2401 ttgtatctat ttaaatatta gaacattttt tcttacttct aaatacagta agattgctta
     2461 aataccaaat aggagcaaaa aaactctgta atctaaaaaa aactacaaca tctttatgag
     2521 aattaaaaaa tgatagacta tgttatcaac attaagaaac agatagaaaa gaaacagagt
     2581 atgtgctaaa atgggaacat agaacagaat gacaaattgg aatttatgga ggacattaaa
     2641 tatggagtat ataatcaatc cttgtagtgg tataaaaatt aaaataacta gagggcagaa
     2701 aattgaaatt gtggatattg aaggaggaca ggtcgttgat ttttttgcag aaatgaaaga
     2761 tgattcaact gaatttatat ctccaggtgt tacaattgat tgtaatgagt cattaagatt
     2821 acaagttggg aatttaatat attctaataa gtatagacct atgtttaaaa ttttatatga
     2881 tgatgtaagg gaacatgact tgctacatcc atgttgtaga cctgagatgt acgatttctt
     2941 ttatcacaat ggagaaaatc atccaaactg ttttgataat attaatcgca cacttggaac
     3001 ttcccatcaa attattcacc ccattaattt gtttatgtac acaaaagtca atacagatgg
     3061 gagtattgaa gtatgtgaac ctttatcaaa agccaatgac aaaattattc ttgaagcatt
     3121 ggaaaacatg actttaggga ttgctgcatg cagtgtttca gaaagttctt gtaatagcag
     3181 aaaatgtact tctattaaga ttttggttga ttgacaaatt tcatttatcg aaaaaatatc
     3241 aaaattaaaa aatgacgtca aaggcgcaaa agggtaacca cttgcgctct caattcatta
     3301 tggtttgtta cgtatttatg ggtaaaaatc ccttgattct atgatgtttt tcagacacaa
     3361 ttacaaatga gtaaggattt tatactcata ctctaaaaaa tagcatttta ttgcgtaaca
     3421 gaacgaaaaa acagtgtgta cctttttctt gcaatacaca ctgtattcat agccactcta
     3481 gctgttaact gttaattatc tcataatacc cttactggta ttcatctaat cttaacccct
     3541 tttaattatt ttcatttata aataaattct attcttccca aaatatcgta attaaaatat
     3601 attaattaaa atatcccaat aagaatatct gaaaaatctt ctacctattt tttcaaacct
     3661 tttattccca ttcctcatac aatagtgcaa cttttagcac agtataaata taccattagt
     3721 ccatataatc tcattaacaa atatatacaa ttattttctt caaactcttt tatatctaag
     3781 atatcacatt ttcattttct tatattaatt ccttaactct ctttcaaatt accaattttt
     3841 taatcaaatc ttcattaaat attctatcat tattaaacat tcatcattat ccctaaaaaa
     3901 tatgtgccgt atgttcttta cacaaatttt ccatcttatc atacacagca ccactatctc
     3961 tctcatctgt catagtcaca attacatcac ttggtctaag aattgtctta cctctaggaa
     4021 taatttcttc tccacctctt tgaattgcta ccaaaagaca attttcaggc cactgtacat
     4081 cctgaattgc cttgttcgca acaggagagc caaacataac agagaactgg tctagtatct
     4141 tttcgcccct ctctttatgt acaggttctc cattcttttc aagtaacctc tcaagaagac
     4201 tttcataaat aggtttagac ttcataagtg tagccacaat atatgccaca attgaaatta
     4261 ctgatagtga aagcatttga ctaacagaac ctgtcatttc aaaaattaat atgataccag
     4321 tcaaaggggc acgcacaata gctgtaaaat atcctgccat agccaaaagc acaaagttat
     4381 ttacataatc gggattcatt ccaaaaactt taacacctac cataccaaat attccaccta
     4441 taaatgctcc taaaacaagc aatggaaaaa atattccccc tggtgcacct gacccaaaac
     4501 taacagccga aaatataaaa cgtataacaa aaatcaaaac aaccattcca aaaatatatt
     4561 tatgacttgt aagttcttca actaaattgt gtccactccc cagaacatca ggcataacaa
     4621 gcccaagaat accagccatt ataaatggaa tcaacagttt tccaaacgta ccaattttag
     4681 gaatcttctt gtataaagac tgcacatata gagtaaacca gttgtaaaat gcccccataa
     4741 ctccaagaat aacacctaaa atcactatta atccataata atcttgtggc aacacatttc
     4801 caatctgaac ctcccatgac taaagtcaca gggttcctgc ttcatagaat tttgcatact
     4861 aaaatatgtc ttactaattc tccacaggct atccccgtag ttcctacggt tcttacataa
     4921 gttatttatt ttgaattgtt attagtctta atccttcttt taatatattt atacttgcat
     4981 ttatatctct atcgtgagtt tcattacaac taggacaaat ccattctctt ataataatgt
     5041 taacaaatgt aattatttaa aacaattatt tagaaggttt tatatttaat tttaaataac
     5101 atatttaaga accgtaagtc ttaactttta tcatttttga ggttgtcgtt cacataagtt
     5161 cgctactacc tatgcagttc tcttatgaac ttcttacact ttcatgcaag cacagactat
     5221 atcttatcct tcggcattat ccgttaaggt ctacccactt ccactaccaa tagcttgtag
     5281 tgtacttccc tcaagaggaa tagtcgttga agtttctcct attcggagtt tacctgctga
     5341 ttgcccattt taaagtactt agggtttaac cttataccat ctaaccaatt ttttctactt
     5401 tcgtaacttt cacacttagg tttatttcat ccttatgttt tagtttggtt agctttaggg
     5461 gtttccagca attcaagtag tattggatag gttctatcct atctctacat ataggtttcc
     5521 ctatatgctg actattttag ctactaactc atgactaaag tcacaagtgt tcgtagctat
     5581 tttttaatca aactggaata cagactccat tcctaggaaa ttggctgaaa tatagtctgc
     5641 tgtcaaagat gctgtcatca cagaaattaa aacagatact gaaaagtttt tatggacttc
     5701 ttcaagtgaa aacataacac ccgctagtgg tgcatgaaag gctgctgata atccagcact
     5761 tgccccacag gttaacagga atttttcctc tgtcttccct cggtcaagag ctctagaaat
     5821 acctttacct gccatagcac caagctgaat ggaaggacct tcccttccca aagcaagacc
     5881 acccatgagt gataagaatc ctccaataaa ttttgttggc aacactcgcc accatacttg
     5941 ttttattttc cctgtcattt ccccctcaag ctgagggata ccacttccag aaatcattgg
     6001 ctcccagtcc accaactttg atacaatcca cgcaagtact gcaagtactg caaaccaagc
     6061 tattattttt attgtagaac cttctacaaa ttttaaaatt tggctaagcc aagtaccagc
     6121 ctgccctaaa agtatcctgt ataaaagcac aatcaaacca gccacagccc caaccataag
     6181 tccttcacca atcaaaatta cctgaaagtg ctctgcccgt ttgataatat gagatgtatc
     6241 ctttttcata ctattcctcc ttcatccaat aattctacac aatctgaatc tccaatgact
     6301 aaagtcatga gtgtgtacag atatttttta atcaacaaaa atagattttt acttactaaa
     6361 tataagaaaa taattgctca atttctcaat aaatgtgaat ttattctttt aggtatttat
     6421 tctccacagt cgtgcttctt ccagcaaaat atttattaat tcttgctttc tctgtgtata
     6481 gattttacga ttgtcctcaa actgtaatgc taatttttgt ttatgactat catagagttt
     6541 tgctttgtct gtaaaagtat ttagataatc tcgaaaattg atataattat tccatgcaac
     6601 actattccat tcaacaacat gaatatggtg agtacgagta tttttctcaa aatctcccat
     6661 agcaaaaagt aattgttttg gtacatcctg acctcgaaaa acgattccac attgctctaa
     6721 cctttcgagg tataatttag cttcctctaa agaagcaaca cctacaacaa tatcaataat
     6781 aggctttgcg ctaatactgg gaatagaagt gcttccaatg tgttgaatat caatagcagt
     6841 cttatcccat atacttttta ataatataat gacatctttt gcacttttat cccatatcat
     6901 ctgatgttcc aataatttaa ccataccaca ttttaaacct atcactatat accacctcaa
     6961 tataatccac ctttatatat attacaattt tttctatctt gataaatagt gatttagcta
     7021 tttcactttt tcttgacact cgctacattc cctatcatga atagatataa ttcctccaca
     7081 tttagaacaa gtatatctct ccttctgctt ttgcataaac ttctctaaac cgtactgctg
     7141 aacaaattta ctattttcca taaggcttgc ttgatatctc ttgttataac ttttttcaag
     7201 attttttatg agtttacaag gaaaattgaa acattcaaaa caataaaaca accctttttg
     7261 acagacacaa tcctttattt tgcacttacg gcagtgttct ggctttcctg tatcgccgtt
     7321 caaacaacca gcacatggct ttttgtgata acaatgctta tagcaaacca tacaattcat
     7381 tccacaagga gcaaacataa tcgtatcaat actctctttt ggcattttca taacatttta
     7441 ctcctttcaa aaatccaatt tgtcgaattg ttatatattc atattgtaac ataaaaaata
     7501 gctaaaaaat tcatgcactt tcattgccaa taaattttca aggaaatatg aaagtttaaa
     7561 actaagaata aaaagagata aaaaggaagc tactaatcaa aatattcaaa cacaagtaat
     7621 caatataaat aacttaaggt atatttaaaa gtatatgata ttaccagcag ttttaaatac
     7681 aaatataacc acacatgcct caaaaacact tattacagta tcttagaaac ttagacaata
     7741 cataagattc attaataaaa aagttctcaa tatagatata tttaaccatg ttttgaatag
     7801 tagataatca tatgattaaa aattaaatat gacatagcgt ttgtggaata taacacctaa
     7861 attatgttat aataataaag caggagtgtg atagtgatgg atgaaaaaat attagagtta
     7921 ttacaggaaa tgcaaggtaa tattagtaat atgcaaggta gtatcagtgg tatcgaaggc
     7981 actattagtg atatgcaaag caatattaat ggtatcgaag acactattag tggcatgcaa
     8041 ggcactatta gcgatatgca aggcagtatc agtagtatga atggcactat tagcgatatg
     8101 caaggcagta tcagtagtat ggatggcact attaatagta tgcaagacag tattactgat
     8161 atgcaagttg aaattaaaga agtcaatagt aggttagaca atatggaacc tgatattaaa
     8221 gacttaaaaa ctggacaagt tgaaactagt agtagacttg atactattca gagtgatgtt
     8281 acagaaatta agtcagatgt aaaaattaac agaaatacat tagatacagt gattaaacat
     8341 acctcttcat tagtaaaaga tacatcagat attaaaaatg atttaaataa tatagagata
     8401 gtcacatcta agaattcgaa tgatattgct aaattaaaat cagttaaata atatactata
     8461 gtaaaaaata cctgaaaatt tcacatactt ctttaaagtt ataccaaatt ttatacagaa
     8521 atactgatta tccgacaaca cactttactt ataaataatt tattaaaaaa atactcattt
     8581 tatcctaaaa ttaacacgac taaatgagtc ttgatataat aataaaaaat aagggagatt
     8641 gaacactaaa aagttgtgta tctcccttat attctataat atagttatat aatttttcag
     8701 cctaaaataa aagtccatct ttgattaaaa cacatcccct tatcacttat aaaaggataa
     8761 aatcttgttt tcatttttcc atttatatct tcttgcttaa gagtaataat tttttctttt
     8821 gatactttaa gtgattggtt aaatttaacc aaacgactaa gttttgttca acatctattt
     8881 ttcatatttt ccaatactta atctattttt tataaataat tcttttattt ctaactcact
     8941 tacatttttt acataaaacg aagcatataa atttcaacct tatcttgtat ggttcttaat
     9001 acttaatgag tagcttaagt atcaatcatt ataaattttt cccttacatt taacactctt
     9061 accatttcca aaatatacaa aatatatttt gaacatttgt acaatgatga aatacaatac
     9121 atgaaaatac aatatcataa ctgttgtcta aaaatagaag ttcttcaaca taacctttca
     9181 caaaaaacat attatctaag tattttttgt ctgctccttg ttttcctgtt tgtagcatat
     9241 aaattactgc atctaaacat ataactactc acacgaaaaa agaaaataaa cttccaaaat
     9301 acaagctcta aaaactacct ctaaagttgt ataatgttta ctaggaataa ggttgtataa
     9361 catatagttt aaataatcat tttttaaaat tgcaagttat tgctttcaaa attagatgtt
     9421 tttatatcaa ataaatgatt aagaatttct aaatttaatg gctttataat gcactccctt
     9481 tctttgggat aaaataatgt tgcacagata ctttttctaa agataacaat tttatttttc
     9541 tatcaattcc accagcatac ccagtcaaac tcccatttgt tcctacaaca cgatgacatg
     9601 gaataataat agagattcca ttatgcccta ctgcaccacc gattgcttgt gctgacattt
     9661 tagatattcc tttttgttga gcaactttat atgcaatttc tccataagtg attgttttac
     9721 catatggtat ttgttgtaaa attttccaca ctgtagtctg aaaaggagta cctaccatat
     9781 ggagtgatgg tgtaaaattg ggttcttttc ccgaaaaata aatatctaac catttctttg
     9841 tctgctcgaa aattggcaat tctttttctt gatgttcttg gtcaagatta taagcataat
     9901 acttttgatt ctcaaaccaa agaccagtta acccaatatt atctgatgct aaaagaatat
     9961 ttccaattgg actatggtaa tagctaatat attgcataca attcctcctt atattctgaa
    10021 aacttcactc aaatcattag acacttcttt tctatctacc attatgtaaa aacatactaa
    10081 tataatttcc ttctaactac actttcgtta taatatctat cctcatatca tattagtttt
    10141 atatttacct gcatctgttc atcctttttt ccctttccct ttgatgaata taaaattcat
    10201 acatttaagc ttcctactgt attaactttt atagaagtgt atattataat tttatcacta
    10261 aataggccaa aaaaatatcg atatagcatc aagtaatatc ataatacttt caaatgggaa
    10321 aagtaagaat aggctgtaaa aaagaaaagt atttttatgt tagaaatcca gtaaaatcat
    10381 tagtagatag aataacagaa aacgaacaat ttgctattta tcattttaca cataaatata
    10441 attgacgcaa taaagtgttt tctattctac aacgattaat aaggttagaa aaaagtaaaa
    10501 atgaactgta tatgtatgaa gcattagttt ctgtttcttt cctttggctt attatgataa
    10561 aaaatattgt tttgctacca ataaaacaca aaaatataat taatatccat atgcaaaaaa
    10621 ttttacgtta tattgaaaaa tactattgtt ttacattata ttgaagggta ctattgtgaa
    10681 aacttaattt tggagtacta tcaagcagtg gtaatatcaa taaatcagag tgctccagat
    10741 gatttgaact aagtttaaat actatacctt ataaatattt tactgagttt tgtttattaa
    10801 aagcaactca atttctaaaa ggcacaaatg agctaataag tgacattgct ataaaagttg
    10861 tttttcatca aataatctat tttagtaaat gttttaaaga aaaaacggga tattctccaa
    10921 aagtgtacag aaatataaaa aaagagatat gattttttat ttcttttgat tatccaaaga
    10981 gtaaataaaa aatattgtaa aaaacttact aacaacggca atgctttgaa ctatggtaat
    11041 actttgaact tttgattgga ctttttttgc taaaataata tatttaaata gcaaagtaat
    11101 ctgtttaatc aataaaaaat aagagataaa agaaatcaac taaaactttt acctcttatt
    11161 ttgatagttt atcagagcta atatttacac tctttttatt tttgatactt cattaaaaat
//
LOCUS       DAYATN010000126        11186 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.126, whole genome shotgun sequence.
ACCESSION   DAYATN010000126 DAYATN010000000
VERSION     DAYATN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.126"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1691
                     /locus_tag="ACGI8A_002994"
     CDS             <1..1691
                     /locus_tag="ACGI8A_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0180981.1"
                     /translation="NIFYILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISIS
                     IGLILGGIIASLSREYVIGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPL
                     MLSVKTDPMICIRRNNEKVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIA
                     SMVIIFFLMATIYTKSTSNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQE
                     IKAIDGVTKVNSYRHKWFNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSF
                     EFLGIEDTDILEDFLINGKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDS
                     KTNNFTKTISQEFIVCGIITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTS
                     KLANDKYVESELNKITEKTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLF
                     NCCNTIVTSINSRSREFALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRS
                     IIIKPFESINLINWKFIGAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            2022..2366
                     /locus_tag="ACGI8A_002995"
     CDS             2022..2366
                     /locus_tag="ACGI8A_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180982.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
     gene            2604..3290
                     /locus_tag="ACGI8A_002996"
     CDS             2604..3290
                     /locus_tag="ACGI8A_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0180983.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKNLLTVR
                     RMGYKWNVIK"
     gene            3301..4542
                     /locus_tag="ACGI8A_002997"
     CDS             3301..4542
                     /locus_tag="ACGI8A_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0180984.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            4613..5296
                     /locus_tag="ACGI8A_002998"
     CDS             4613..5296
                     /locus_tag="ACGI8A_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0180985.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            5293..7626
                     /locus_tag="ACGI8A_002999"
     CDS             5293..7626
                     /locus_tag="ACGI8A_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0180986.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSRLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(7734..8296)
                     /locus_tag="ACGI8A_003000"
                     /pseudo
     CDS             complement(7734..8296)
                     /locus_tag="ACGI8A_003000"
                     /inference="COORDINATES: protein motif:HMM:NF037635.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8410..9759)
                     /locus_tag="ACGI8A_003001"
     CDS             complement(8410..9759)
                     /locus_tag="ACGI8A_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0180987.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKFFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(9796..11016)
                     /locus_tag="ACGI8A_003002"
     CDS             complement(9796..11016)
                     /locus_tag="ACGI8A_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0180988.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIEIKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
BASE COUNT         4299 a         1326 c         1677 g         3884 t
ORIGIN      
        1 ataatatttt ttatatatta gtatcaaata gaataaagga ttttgggata tttagggctt
       61 tagggtttaa tccaaatgat atttttaaaa taatgatttt agaagtatct atatactcta
      121 ttataagtat ttctataggt ctaattttag gtgggataat agctagttta tcaagggagt
      181 atgttatagg agtaatatac aatgtcaact atataaattc tataaaaaac cagaactata
      241 taaacacata cataatttct atattattaa gtttaggaac tataataata tcagtttcaa
      301 aacctcttat gttatcagtt aaaactgatc caatgatttg tataagaaga aataatgaaa
      361 aagtggatat aaaacaaaat tcctttataa ataaatttat gataaaactt ttcaaagact
      421 atggtaacat agcatcaaaa aatatacaaa gaaataagaa acgtacaaat ttatctatag
      481 catcaatggt aattatattt ttcttaatgg caacaatata cacaaagtct actagtaact
      541 ttttaagtga tggtgtatta aaatattgga tacaaggaga ttatttatta cataatattg
      601 atataagtac tatacgttca aataataaag gttatgataa aaatgtacta caagagataa
      661 aggcaattga tggtgtaaca aaagtaaact catataggca taagtggttt aatataaaaa
      721 ttgatgataa aagtataaat aagaactctg attattggaa aaaaaataaa gataatattg
      781 aagctagatc agaagtaaaa gatggaatta aggtatataa taattctttt gaatttttag
      841 gaatagaaga tacagatata ctagaggatt tcttgataaa tggaaaggaa aatatagata
      901 aatttagtaa acaaccatat ctgtatataa caaagaaatc cagtgattcg cttaatataa
      961 aaaaaggaga tagaataaaa gtatactttg atattataga ttcaaaaaca aataatttta
     1021 ccaaaacaat atctcaagaa tttatagtat gtggaattat aactacatta ccagtaacct
     1081 cacaagtggg agctgaattt ggcggagtaa tatcaacaaa tcaatttaat aagtttacag
     1141 gaatatcatc ttatgaaaga tttgacatat ggacgagtaa actagccaat gataaatatg
     1201 ttgaaagtga gttaaacaaa ataacagaaa aaacaaataa aggaatttta attccataca
     1261 aagctgaaac agctgaatat gaaaaaattg ataatcaaaa aacactaatt atggtattag
     1321 taactggaat tatagttata ttatctttat ttaattgttg caatactata gttactagta
     1381 ttaatagcag aagtagggaa tttgcactat ttagaggaat aggaatatca aaacatgaaa
     1441 taaataaaat tgttaaattg gaaagttata tatatattat agtaagcttt tgtatatcta
     1501 taatacctat tttaattgta agaagtataa taataaaacc ttttgaaagt atcaatttaa
     1561 taaattggaa atttataggg gctattatac taatagtatt tattttagta tctataatca
     1621 tgataactac actaaaaaca ttgaataaaa ttcaaaatga aaattttatg gaagaaataa
     1681 agactttata gtgaacagcc tataaattaa agaggtttta tatgaattta attgcaatta
     1741 tcgataatgg tgtagatatt gattttaaat aatttagaaa taaaaataaa ataagttatc
     1801 caagttatat gtatgatgta ataggggtac aatgtatagc aaatataaat attggcttaa
     1861 taaaactcta ctcttaagtc tactgtcgtt ttaacacaag actaataata agtgtaacct
     1921 aaaaattatt gataatacaa taaccttgtt tatgataaat aacaaatatt aagtgttgta
     1981 aagatagtaa gtttaaaaaa atatcaaaga tttcaatcac aatgtattgt ttatctgtag
     2041 tgttagctat ttatggtata tatgtattta taaattgtac tgaatatata tatactatgc
     2101 tatctacaaa acatttaaca gtcaaaggaa atgaatatac cattataaat tattatatga
     2161 gtaactttag tcagtatata atatttgcta ttttacttgc atcaatggga tttgtaatac
     2221 aaaagatagt gcatatgcat ttcagtgaga tagaacttag tggtaactat tcaacaactg
     2281 ttagaaatat aagtgatatc tctttagata atgataatga aatgtttaat agaggcaata
     2341 ttgaaaatag tattttaaaa aaataaataa tataaatata taaaatgtga ttgaataaag
     2401 cttagaatac ttagtgtatt ttaagctttt tttattatta agatttcaaa gtattaaatt
     2461 aactatataa aatcatataa aaataatttt aaaatattta aatcattgat ttcaaggtat
     2521 tgtattttta aaagagtaaa aatataataa gttaggtata taatatatat aaagtaaaaa
     2581 ataaaaaaat ggaagtgatt gagatgagta gtatattgtt ggttgaagat gatttaagtt
     2641 tgatatatgg attagaatat tctatacaaa agaatggatt tagtgttgat gtagcaagaa
     2701 cagtagaaga agcattgcaa atttataaag aaaaaaatta tgatttactg cttttggatg
     2761 tatccttacc tgatggtgat ggttttgata tttgtaaaag ggtaagagaa gcttctaatg
     2821 taccaatcat atttttaact gcttctgatg aagaagtaaa tgtagtgatg ggacttgata
     2881 tgggtggaga tgattacata acaaaacctt ttaaattaaa tgagttaatt tcaagaataa
     2941 aagctttact tagacgttat aattttacaa gtgaaaatgt aactgaacta aaatcaaata
     3001 atattactgt taaattactt gaaaatcgtg tttttaaaaa tgaaattgag cttgaactta
     3061 ctacagcaga atataaatta ttatgcttac ttatgaaaaa taaaaatatt gtacttacaa
     3121 gaaaaaatat attggataag ttatgggatg gaaatggtag ttttgtagat gataatactc
     3181 tgtcagtata tgtaagaagg ttaagaaata agattgaaga taatcctgaa aatccaaaaa
     3241 atctattaac agttagaaga atgggttaca aatggaatgt tataaagtga gggtgttaat
     3301 atgaaggtat tttctaataa agacataaaa atattttttt tggtgattat atccgtttta
     3361 ttgatgttta tagtagtagg tcaaatagta gttataaata taactaatga ttataaaagt
     3421 gcactgttag aacatgatta taatatagct ggatacttaa atagtatggg tgttgataaa
     3481 tcaaaaatac cagcagtatt tactactaat gaagataaat atactttaaa aggaaaagaa
     3541 atcttaaata cagctagata tgactttggt acagatgata gttttatgcc aagtgtgaaa
     3601 ttgtttcaag gtaaatatat aaaaactatg tttttgtatt tatttgtgtt ctttactatg
     3661 attattttta tattgtatgt gtattttttt aggcaacaga acaagataga agaagctagt
     3721 tataaaattg attcatttat gaatggaaat ataaatgtac ggttaaatac ttatgaagaa
     3781 ggtagtttat caaagttatt tggttcaata gatgctatgt caacatctct aaacacacat
     3841 atattgaaag aaaagcagaa taaagaattt cttaaaaata caatttcaga tatatcacat
     3901 caactaaaaa ctccacttac agcattgatg atgtataatc aaatattgca ggaagagagt
     3961 cataatagtg aaattgttga gaattttgtg cagaagagtc aaaatgaatt agagcgtata
     4021 gaatcattga tacaaaactt gttaaagatt acaaagatag attcaaagac aataattctt
     4081 aacaaagcta gtgttaacgt aaaaaagttg attactaaaa ttttatatag ttttgaaact
     4141 agagcagaaa aagaaggaaa gagtatctct ataagaggtc tagaaaatac aaacttgttt
     4201 tgcgattatg aatggatatc agaagcttta agcaacctta taaaaaatgc attagataat
     4261 acaaaagaga acgataaaat aatgatagaa tgggttgaaa cacctataac aactattatt
     4321 tcagtgagtg ataccggaaa tggaattcat attgaagata tacatcatat atttaaaaga
     4381 ttttatagga gtaagttttc aaaaagtaat caaggccttg gattagggtt gccactagtt
     4441 aaatctatca tagaacagca caatggaacc ataactgtag aaagtaattt tttacaagga
     4501 agtacattta ctctaagttt tttaaagctt actaatatgt aagataggat acatcttaca
     4561 gtaaggtgta tgaattattc ttatattaat catcagaaag gagatagagc aaatggatat
     4621 tcttgtggtt aataatttat gtaaaacata tggaaatgga tatacaaaag tagaggcatt
     4681 aaaagatgta tcatttacag tttcaaaggg agagtttgtt actgtagttg gagaatctgg
     4741 ctcaggtaag agtacattat taaatctttt aggaggtctt gatactccaa cctcaggaaa
     4801 aatatttgtt gatggtaaag aaatatgttc aatgaaagaa gaaaaacaga ctatttttcg
     4861 acgtcgtaat ataggtttta tctttcaatc atataatctt atacccgaat taactgtgga
     4921 acaaaatata atatttcctt tactactaga ttacaaaaaa ccagacacta tttatctaga
     4981 tgaagtattg acaattttag gtcttaaaga tagaaaaagt catcttccaa atcaattatc
     5041 tggtggtcag caacaaaggg ttgcaattgg aagagcattg attacaaagc catcaattat
     5101 attggcagat gagccaacag gaaatcttga cagtaaaaat agcagtgatg taatttcact
     5161 tttaaaagtg acagcagaaa aatataacca aactattatt atgataactc ataatacaaa
     5221 tatagcttct acagcagata ggatactaca ggtttctgat ggaatgctta aagacctagg
     5281 ggtgaataga gaatgagaag ctatttagaa ctagtaccac aatatgcaaa ggtacataag
     5341 aaaaaaaata gaactacaat tttatgtatt gtcattgcag tatgtttagt tactgctatt
     5401 tttggactag cagacatggc tatacaatgt caaaaagtac aatttataaa agatgatgga
     5461 aattggcaca tagtacttaa aaatcttaat gaagaagcta tatcagaaat aagtagtaga
     5521 gtagatatag ctcactatgg tagaggacaa gtcattgata atggagagtt taaatcaaag
     5581 gagattttga tacaaggttt agatgaagat ttagcaaaac aattaggtct agaaataata
     5641 aaaggtcgtt atcctgttgt ggagaatgag actttaatag atgtagatgc agttaaaagc
     5701 tttaatctaa aagtaggaga ttttatcaaa ataaaattag cagatggtag aaataaaaat
     5761 tataagatag taggtgaata ttcatcatta tcaagattaa gagcctcaga taagcatgga
     5821 ttaatgcttt caatattagg tacacaagct atcaaaaaat cagaaacata tgattattat
     5881 tatattacat ttaaagacaa ggttaatatt agaaaagcta cttctgatat taagaatacc
     5941 ttcaatttaa gtgataaaca aatcggtaaa aatgaaaaat tactttctat tataggtcaa
     6001 ggtgactctg attattctaa accactttac ttaacagcag gaggactttt tatacttgtg
     6061 ttaatagcaa gtattatcat gatatataat agctttaata tgggtgtaat agagagaata
     6121 aagttttttg gactattacg ttgcttggga gcatcaaaac tccaagtcaa aaaatttgtt
     6181 gttttagaaa gtttaattct aagtgtaaag gctattccac taggattgtt attaggatgt
     6241 attgtaacca ttatatcctc tatatttctc aaatatgtga atacagagtt atttggttcg
     6301 atgccaattt taaaggttag ctttattggt attgtgtttg gaataatagt aggattttta
     6361 acagtaatat tatcagctat tgttccagca aaaaaagctt ctggggtgtc tccactaagt
     6421 gctatacgtg gtaatctaat gttcaaagaa attttaacta gtggaaaagc aagtaaaatt
     6481 gctaacgcag gaagttctaa aattgatgtt tctatgggaa taaatcatgc cttttcaaga
     6541 aaaaaatctt ttatacttat gacttcttca tttgctatta gtattgtatt gtttttaggt
     6601 tttagtgtat ttatagactt tatgtatcag gcacttaaac ctataaaacc agaagctgct
     6661 catatatcta ttgttgacgc tgactttaag tctgttctta ctaaagacga tattaatgaa
     6721 ataagtaata tcaaaggaat aaaaaaagta tacagtagaa tgatgacgga tgtagaggct
     6781 acatacaata ataaggaggc aacatctcag ctaatttctt atgaaaaaaa tcaatttgac
     6841 tgggccaaaa agtatctagt aaaaggggaa atcaatgaag ataaattaga gtcagaaaat
     6901 atcggattgg tagaagaagg tcatgggttt aatattggag ataagatata tattaaaaat
     6961 ggaaattcta agaaagagat agaggtagca ggtatacttt cttcaatttc atttgatgtt
     7021 aaagacaaat atattggaaa tattataaca tctgaaaata cttttactag tataacagat
     7081 ataaaagact atataataat tgatactcaa gttgatgaag atgctcctga agatttaatc
     7141 caaaatataa gaaatgttct agatgataat ttagaggttc gagataagcg acaaggtaac
     7201 ttagaggcta aaaattcatt ttacactatg gcagtttttg tatatgggtt tgtatttata
     7261 attgcgatta taagtatgtt taatatcata aacagtatga atataagcgt tacaagtaga
     7321 ataaattact atggtattat gcgtgcaata ggcatgtcca ataagcaatt aagaaaaatg
     7381 gtaatagtag aatcaagtac atatgctgta agtggctgtt tattggggag tgtacttgga
     7441 ttaatattac atagatatat atttgtatcc ttagtaacat taaaattcca tatagactgg
     7501 caaatacctt ttgatttact gtttatcatt gtagtaacta tgataataat aactttgctt
     7561 gcagtaagaa aaccaattaa aaagatttgt gaattagaca taatagaggt agtaaatgca
     7621 caataaatat aagtagtaat ttaaatagag gtagtaattt aaataaaaaa tcgaagtaaa
     7681 tatgaaatag aaaatatctt tcataattta cttcgatttt tgtgttatat aatttacttt
     7741 agataagttc catcttcatc ttgataaatt aagtctttct tcataagtac tccaagagca
     7801 cgtttaaaat tttcttactt gaattaaata agatagaaat atctttagga tcagatttat
     7861 cattgaatct catatatcca tctgaacctt ctaaatagct taaaatctta ttttctaagg
     7921 tatcaagttc atcttttctg tttcctctag gagaaagtcc taatttacca tcatcgtata
     7981 tttttataac atgtaaattt tcaagaacat ccccagcttt taattctgta aagtattcat
     8041 tatgaaggat tacaccagca tatttattgt caacacatat catagcagag ttgttggttt
     8101 ggaatccata tacaactcca tttacaacat ctcctacatt gtaagtatgg tctgtttcta
     8161 aatatgaatc tatgtctgta gtagcagcta ttcttccgct tttatccaag tatatataga
     8221 ataaatattt ttcatctcta aacaatggat aagtcttagc tttaaaagga actagtatgt
     8281 cttttgtaag tcccatatct ataaaagtac caatatccgt gttatcaact acttttagat
     8341 acgtaacatc tccatttcta attatttcta tagtattaga aaaataattt ttaaatagtt
     8401 tgtaaaatat tattgaattt tttctctcga aatatataat ttagaatctt ctgatttatt
     8461 tttatgctcg atttccttat tttttttcat aaatatatat agaggagcta ttaacaacat
     8521 tgcccctaat cctattaaaa ctctattttg agttataggt tttgaaatat cttctgccca
     8581 agcttgttgt aacaaccata agctacctaa aatagctacc attggtataa caggaccaaa
     8641 tggaatctta aaagaggcct taacatctgt tctttttctt aaaactatta ctgataaaca
     8701 agttggtatg tactgagcaa atcttgcaat tacactcatt actgcaagtt gttcaaaact
     8761 tccagttagc acaagtggta tacaacaaat taaagatatt attattgcta catatggagc
     8821 accatattta tttgtttttc ctataaatgc aggaagagaa ccttcttctg ctagagaaga
     8881 accacattta ggagttacta ttgatgaacc tatatttata ccaaatatag atattaatgt
     8941 tgctacagat atgaaaaatt taccataatt gcctaaaaat acacttgaag catctgcaat
     9001 tggaatactg ttttcaaata gtctatctcc aagtataccc atacagacaa tttgaattaa
     9061 tatataaatt attgcacata taaatattgt ggttattaat gcgactggaa gatttttttt
     9121 aggattttcc atttctccac ttgccacaat aaatgattca aagccggtaa atgcgtaaaa
     9181 tactaatata atggctggac ccatactaga gttaactaca tttcctgaag gaacaatatt
     9241 tacaaatttt ataaagaata ttccaactat tataaaaact attaatggta ctaatttagc
     9301 tatagttatt acattattca ttatctttgt gcttttaatt ccaaataagc tatttattga
     9361 aagtagtgtt acgagtattg cagctatata acctttgtat tcagtggcag attctggcca
     9421 aaatgaacct aaagctgttg caaatcctac tgctagagta gaccatgata taattctaat
     9481 aacccaagac attgtaccaa cttcaaatcc tataaaatct ccaaaagctt ttttggagta
     9541 tagatatgct cctccattct tatcaaacat gcttccaact tctgcaaaac ataaaagtat
     9601 tgatagaact aagagtgtag caaaaatgta tataaacata ctgttttgac ctgctagatt
     9661 ataaacttta cctggtagta agaatatacc tgaacctatt atgctattaa tccctaataa
     9721 tatcatacta aatagaccta atttcttagc tttttccata ttaatttatc tcctattctt
     9781 ttattaatta ctgtactaat taaaatacaa agtattcatt acatgcatca taacacctat
     9841 tggtaaacaa tcttcatcta tattaaagct agaggtatga agaggtgaaa ttaagccctt
     9901 ttcctcattt ttacatccta aatggaagaa tgctccttta caatgttctg tgtaaaaaga
     9961 aaagtcttca ccacctaaag acgggtttgg tctcaatata aatttttcct ctccaagtaa
    10021 ttctttagta tttgatataa ctgtatctac taattctttt tcatttataa cagcaggata
    10081 gttttcatca cttacatgta aagtcccaac acaaccaaat gcagatgcag tgtctccaac
    10141 aattttagct attttatcaa tcataaagtt tcttgttttg ctatttaagg ttcttaaagt
    10201 gccttctagt tttacatctt cacatattac attgtgtgca tcaccaccat atattttacc
    10261 tatagtcaaa acagcagcat tatttggttc taagtttcta cttattatag tttgtaatga
    10321 tgtaacaatt tgtgaagctg taactattgc atcaatacca ttttcaggat atgcaccatg
    10381 agctctttta cctttgatac ttatttttat agtgtctaca ctagcattta atgtatcata
    10441 ttttatttcg ataaatcctg tttcgatatg tggcataaca tgaagtccga atatataatc
    10501 gaccttagga ttttctaaac atccatcttc aactaagaat tttgctcctc caaaaccttc
    10561 ttctgctggt tgaaataaga attttacatt tacattaagt ttatctttta ttgaatgtaa
    10621 aactttacat gctccaagta gcatggttgt atgtgcatca tggccacaag catgcatttt
    10681 tccagaatgt attgatttat aaggtatgtt gttttcttct tcaataggaa gagcatccat
    10741 atctgctctt atacaaatcg ttttatctgc actttctttt aaaatatatg ccataattcc
    10801 attgtgtttt gtaaattcca tataatcaat tccgatttct tttaaataag aaattacctt
    10861 ctgtttagta agattttctt caagagctaa ttctggaatt ttatgtaatt ctcttcttat
    10921 gtttataagc catggtttta tagaattaca actttctatt acaaaatctt ttcctaattc
    10981 tttggtttga tattttaatt tcaaagcctg ttgcattttt attcctccaa ttgcttttta
    11041 ttgttttatt aatataataa gcaattatta tgccataatt ttcgatggag aactttgttt
    11101 tttataagaa tattgaataa atataatcat ttatctatat gtatagtata aaaaatgatt
    11161 gcatagtttc aattttcagt aaaaaa
//
LOCUS       DAYATN010000127        11096 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.127, whole genome shotgun sequence.
ACCESSION   DAYATN010000127 DAYATN010000000
VERSION     DAYATN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.127"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(43..675)
                     /locus_tag="ACGI8A_003003"
     CDS             complement(43..675)
                     /locus_tag="ACGI8A_003003"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFH0180989.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(768..1100)
                     /locus_tag="ACGI8A_003004"
                     /pseudo
     CDS             complement(768..1100)
                     /locus_tag="ACGI8A_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            complement(1178..1381)
                     /locus_tag="ACGI8A_003005"
     CDS             complement(1178..1381)
                     /locus_tag="ACGI8A_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFH0180990.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1771..2001)
                     /locus_tag="ACGI8A_003006"
     CDS             complement(1771..2001)
                     /locus_tag="ACGI8A_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0180991.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(2003..2410)
                     /locus_tag="ACGI8A_003007"
     CDS             complement(2003..2410)
                     /locus_tag="ACGI8A_003007"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0180992.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2770..3219)
                     /locus_tag="ACGI8A_003008"
                     /pseudo
     CDS             complement(2770..3219)
                     /locus_tag="ACGI8A_003008"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3233..4159)
                     /locus_tag="ACGI8A_003009"
     CDS             complement(3233..4159)
                     /locus_tag="ACGI8A_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180993.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            complement(4284..5066)
                     /locus_tag="ACGI8A_003010"
     CDS             complement(4284..5066)
                     /locus_tag="ACGI8A_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFH0180994.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5222..5842)
                     /locus_tag="ACGI8A_003011"
     CDS             complement(5222..5842)
                     /locus_tag="ACGI8A_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFH0180995.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5969..6637)
                     /locus_tag="ACGI8A_003012"
                     /pseudo
     CDS             complement(5969..6637)
                     /locus_tag="ACGI8A_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6708..7544
                     /locus_tag="ACGI8A_003013"
                     /pseudo
     CDS             6708..7544
                     /locus_tag="ACGI8A_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            8216..8983
                     /gene="aroD"
                     /locus_tag="ACGI8A_003014"
     CDS             8216..8983
                     /gene="aroD"
                     /locus_tag="ACGI8A_003014"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFH0180996.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9311..9988)
                     /locus_tag="ACGI8A_003015"
     CDS             complement(9311..9988)
                     /locus_tag="ACGI8A_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0180997.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10138..10455)
                     /locus_tag="ACGI8A_003016"
     CDS             complement(10138..10455)
                     /locus_tag="ACGI8A_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0180998.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10477..10815)
                     /locus_tag="ACGI8A_003017"
     CDS             complement(10477..10815)
                     /locus_tag="ACGI8A_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0180999.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3690 a         1744 c         1572 g         4090 t
ORIGIN      
        1 tgattattaa agtaaagttt aatataaata aattataaaa tattaaacta atttatcaat
       61 atttcctgat tgaatatgct taatgtttga ttgaattttt ttatatggcc aatcccacca
      121 tttaatttct aataattttg aaattgtatc attattaaat ctctttttta taatttttgc
      181 aggtactcct ccaacaatag aatacggtgg aacgtcatta gtaactactg ctcttgttcc
      241 aataattgca ccatcaccaa tatttacacc tgacataatt atagaatcat aacctatcca
      301 cacatcattt ccaattataa tgttaccctt attgtcccaa gcgtccgtta tatggttgat
      361 atcgagtttc cattcttcat aaaagatggg aaaagtatat gtggattttg atttcattga
      421 gtgatttcca ctagtcatta gaaattttgc attacatgct attgagcaaa acttaccaat
      481 tattaattta tcattgttta tagggtaatg atacaataca ttgtttttct caaaatctct
      541 tgggtcatta taaaagtcat tatatattgt gtaatctcca acttctatat tttcccttgt
      601 tattacattt ttaagatata cagtttgata atcattactt cttggatata ccttatttga
      661 acttggaata gtcatattta aatctctcct ttaatatata ttatgttttt gactattcca
      721 ctactacaat acatctcttc acagaatcac tccttgttaa gaatcttcat aagataatgg
      781 cgtccttttt tagtgttaag tcggatattc tttcttagtc cattcttttt agcgtataac
      841 tgacaaactg tcattttctt accaatagtg tcaatgctat catcaaggtc attttgtatt
      901 gctttttcct tatcccttaa tgatatatca tcacgttttc cctttggggg tttatcagta
      961 ggtacaagtt tccaagagta aacaaattgt ggtttaccaa atgaatatac atacttatat
     1021 gcgtatcttc catcttttct ctggctctct ccagttctta aaattctatc tttattatct
     1081 cgtctttttt cagttgacat gataagaagc tcctctcaat aaccgaattg agcttgatac
     1141 gtcatatgaa tattataaca tataaggctc aatttcatta gataacgtca agtgtatcaa
     1201 cgattttctc aaattgtttt ctctttattt gaatgcgatt gctattcata atgacccagc
     1261 cagaatcaag gttttcatca gcacgtttac gtaatttttt ctctccaata cgaaagtatt
     1321 ttgacgcttc ttcaatcgtc aaggtatatt tttcccaaat aggtacatca gtattgttca
     1381 tataatgtat cactcctttc tatgttcgta gttcagaagt agatagatgg ctttgataag
     1441 ctccattggt atttcaacca atcccattct tatggacaga accgtgtcat acaggtgtat
     1501 cattatactg tggtacaggt tacggcaaaa caaccgttcc aaaggttatt cacaaatcac
     1561 tatatagtag ctctcttgat taaggtcatg gcgtatagta tttgttggct atctgtatca
     1621 caaacgtatc tatctacttt attcagttgt caaagaacag tcggcattga tttgcctata
     1681 aaaaatcaca atagcttgtg ggcttttata gacaaataat tagtgattct ccatgtacca
     1741 aatagcttga tacatggaga gagggcgaga ttaagacaaa ttaaatctca aaatagcagt
     1801 gattagtttt gttcctagca ttcgctttgt ttcttcttct acatgaatat agggatttcc
     1861 gtattcatca aataaagttt tctgtgaaag acgaatgata tagtgttcaa aatgtttaag
     1921 tattacgttt attgcattta catcgccatg tacagcaagt aaaataattg taaaaggtaa
     1981 tacttcttta gtaggtttca tttcaatgtt cctgcatgag ttctttcaac tcttttagtg
     2041 catttgtttt gtgataatgt atggtatttt ctgctaaatc catcaatgtt gctatgtcag
     2101 catttgtcat gttgaaaaag aatgaaagta gaattatatc ttgttttctt tttgtaagtt
     2161 ttccgattgc ttgcgccatt aatacatctt caattcgtat gttgtagtcg taagaaacca
     2221 tacagatgta gttagaatca taatcatcta aaatactcaa tttacttaat tctgcaagtg
     2281 acaaagattc gagagatacc aaataatcat ttctgcgttt attttcgtca taaatatctc
     2341 tagcatagtt acgcaaaact gttttacaga aactgtcgaa ttgatgttca attgtctttt
     2401 catcaaccat aagatttcac cctctttcca tcgcaaggag ttaaataacc ttgcttactc
     2461 ctatcacggt gggagggcgt taaatcttcg ataaatttca aaaaaaagag aaaaaaatac
     2521 acactctgcc tatagggcaa aaatgtgtat tctttaaaac gtatttaatt tatattggtt
     2581 gtaagccacc accatattga tgtatggaaa tatgcattgg ctagcttttc ataaaaatac
     2641 aaagatgcat aagaaattaa tagaggggaa aaacttattc tgttattaaa aagtaggtta
     2701 attctaataa atgctattgc ggtcgaatta aactatatac atggcaatca atcatgtttc
     2761 tattctttat tatgaattgt gacagtattc ttcaatctta aagccacatt tttccaatac
     2821 tttacttgac tctctattgt aggaatatac attagcatga attttgttaa ggttgtattt
     2881 ttcaaaggca attttgcata tttcttttac tgcatttgtt gctatccctt tgcctcaata
     2941 gttcctagca agccagtaac ctaattcagc atttttatta atagaggtat cttcttcaaa
     3001 tgttaggctg atacttccaa tagccctttc ttgataaaaa atagcatagc taagttgttt
     3061 aacatctgaa gttgccaaac aaaaactaac aaaatctcta gcgttttgct ccctataagg
     3121 gtgggggaag ttgtcacgaa gattctttgc aatttcttta tcattcgcaa actcaactaa
     3181 atcggtaata taagcataat tccactctat aagttccatt tttcttctcc ttttatgaaa
     3241 cagctacaag aaatctttct aagccaatac cacttatcaa acagcgttct cttttatttc
     3301 caagcatttg ataagtccag tcaacaaatc caccgtcaac tatttcaacg gtttcatttc
     3361 cttgtttgac cgataatttg aaattgatac ctttataata attattatca acatcatcat
     3421 tatgaattga taactctata ttaggaaaaa taagtttgat agtttcaacc attctgtcga
     3481 aaaatccgtc attatctttg tatccgtttc ttttgcgtag aataatagag aaacgaacat
     3541 tttctatttc acttaatata tctttataat agtttaagtg cttttctaac aacatttttt
     3601 cacaagtgta agaccctgta tctataccag atgaaaccat acaatataag ccaaaatgtg
     3661 aatataaacc tttacctaca aaattttgtg ctcttattac cctagcagta gtagccatgt
     3721 ggataggtat ggtattattt tcttcttttc tttttaattt atcagcaata attattgcca
     3781 acatatttga ggggtctgca agtgtttctg tccctcgtag agcacttact acgttatatt
     3841 gatctacgga gccaaaaacg ctacagctac ctaatggggc agagggagaa agcataatcg
     3901 tcttgatatc catattgaat gcttttctca ataattttga ttccaaagca tgaatttctt
     3961 ctggatctat accgctaggg atagtaaatc tatttagttg gtatgacttt agaatatcag
     4021 ttgcagttaa agtatttgct tgtctatcaa acacttctaa tagaagtgag ttcaaatctg
     4081 attttgatag agctgataac ttatcaacta tattcttatc cccaatttta tttgtaattc
     4141 tacttaaaat atcactcata gttatcctcc atactgttac tttcaaaata attatagaat
     4201 tgttacaaca actaatctag gtcatttgac caaaaatgaa aaaacactga atatttacta
     4261 ttcaatattt ttttactttg ggtttatttg attaaatctt gaaaatactg taatgcaacc
     4321 ctttcagctt caacctttcg gacaatagga ctttgtgata attcctctct taactcaaga
     4381 tacaggttga tagtagcctg caatgcatta acacagcttt tatattcatg gttagcaacc
     4441 gtcttttcta atgcttcaat atattggggt gcgtccaatt caatttttct aactccacga
     4501 ggtaatttac ctaagagaag ctgtattaat ggggcaagta ctgattgtct taaaaaagat
     4561 aaaaaatcaa ttgtttcaaa tagttctcta cgtccaatct tagtgcaagc gtaatgaacc
     4621 caaatccaaa atctatcttc aatccactgt aagtctggag ttgggaagta tgcttctttt
     4681 ttagaataaa tttctgttat gcagttgttt tcttcataga gaatagctga atcttcaacc
     4741 ctatctagta aaccatctaa ggttgtaaac ttaaaatcaa catgaagaac aggggaatca
     4801 tagagggaaa ttatcaatcg aggttctcca acgtgttcgc cagtaaaagc agaaactaat
     4861 gttccaaact gttccacaat aagtaatctc tcttcgataa cttcttcata ggatttatca
     4921 tctgctacaa tcagaaaatc caaatcagaa tattcatcta acgattttgt gatataagaa
     4981 ctgctaattg caagtcctat tatcctacta tctctttttg aaaattctaa tacgttttgt
     5041 atcattttct gttgtgtttt agtcataata taacacctct cactgtcttt tattagctat
     5101 caatccaaca taattatgca taggagtatt atataatgaa gaaataaaaa aaaataggtc
     5161 acttgacccg aaataaaaaa gcattgaata attattattc aatacttttt tgaaattata
     5221 cttatgttgt aggaagattc tttaatacct ctcgctgatt aattacacta aatcttttgt
     5281 tttcataagc aattagatgt tcttgtacac attcatttat agcacgatta atgcttctaa
     5341 ttggagcgtt tacttcgcta gcaagctgtt ttttggtaag cagatttaat tcacttctat
     5401 aatagtgtaa ggatatacac ctcagaagtc gttctttaac acttaaagat gataattctt
     5461 caattaattc agcatttaca actaatttac tagatatttc tcgaatcaga aattttgtag
     5521 cttcaaaatc tccccttaac cagtttaaaa aatcatctct atgaagttta tatacattac
     5581 aatctgtgaa agcgacaatt tcaacgggtt ttttcccatc attaaattgt tccatttctc
     5641 caaaaaaact accacttcta taaagatgaa tcgttgagat gtttcctttc acatttaaga
     5701 tgaatgcttc tgcaataccg tcaatcataa gaattacata ttcattttct tgctcagcaa
     5761 aaagaatagt ctctttcttt ttatacgttt ttttaacaag cttcttttta atatcttcat
     5821 tcataaaatc aattaaatgc atattcatcc ctccttacca aatctttgat taaattatat
     5881 catctttcaa ttagttaggt aagatagtaa aaatatattt acatagaaac tattccatta
     5941 ttattttcca gttgtcattt ttctgcaaaa acttatcact gtcacgactg gagataccag
     6001 taaggaatgt taggatatca atcgctaaac tttcagaatc ctttttacgg ctcaaaatga
     6061 cgtaagggtc aagcaatcct ttgttttcat cattactggt taaattgaca atgtttatct
     6121 catgggctat ttctggtagc gtttctttcc atttgccgcg ttctgcttta gggtcaacta
     6181 tgaccgcctg tccgccgaat agtaccgcat agtaaacaag catattgtta ctgaataatt
     6241 taccaccacc caatgaaccg ataaacgcag acgctaacgc attggtaata gaacccttta
     6301 ccccttgact agcaagggca ggttttagat agacattctt tccagtgtcc agattgtagc
     6361 caagatagat atcctcatgt tcgccaaggg tttgtgtcgc tccaaaacca agtccagcca
     6421 aaaaatccga agtgacatat tgaatataat cattgatgta acgtttgctg acaggaataa
     6481 attcgccatg caatcccaac atatccccaa agggacgcac gagcttgatt gataagtcgt
     6541 cgtaaaagtc tttgacttca tcacaacgcc tttttagctc gtcaagattc tttgtagata
     6601 cacggacaac atagctaagt ttatacatag attctttaca gttaaaagtc tgtttttaaa
     6661 aaaatgtata aattataaat caaaatatag atactatcaa tgacccacta ggtgcaaaaa
     6721 gcattgatat ggttaaatta tcagacgcta aaagctggtc tatccgaatg agtgaagatg
     6781 ggtgtgctta taaatccatt gacaattata agcgctcact gaaagcgtcg tttttcatgg
     6841 caattcaaga cgactgtatt agaaagaatc catttaattt ttagttaagt gatgttttgg
     6901 aagatacaac agaaccaaaa atcgctctaa caccagaaca ggaaatgggc ttattatcct
     6961 ttatggggag cgataagact tattgtaaat attatgatga tgttgttatc ctcttagaaa
     7021 caggactacg catttctgaa ttatgcggtt tgactttaga attggatatg aaaaataaag
     7081 ccattatggt agaccatcaa ttgttgagtg atactaagat tggctattat gtagaagttc
     7141 caaagaccac acagggtaag cgagaaattc caatgactga aagagcttat caatcatttg
     7201 aacgtatcgt ggaacggcgt aagaacgcta aagcagaacc aatcattgtt gatggttacc
     7261 agaacttcct atttcttaaa caagatggat taccaaaaca agcaactcat tatcaaagca
     7321 tgttaagagc gttagtcaag aagtataata agacccatga ggacaagcta ccaaagatta
     7381 cgccacatac ctttaggcat acactctgta caaatatggc taacaaagga atgagtccta
     7441 atatattgca atatattatg gggcataaaa acattactat gacgcttggc tattatgcac
     7501 atggttcatt tagttcttcg agagctgaaa tggaacggtt ataatcctag tagtatgact
     7561 agagtttact acttatttac tacttttgat ggcattctta tacggttaaa tgccaactta
     7621 tgcaaagtat cttccaaaaa gaaaatgccg atactccttg tagatacagg atatatcgac
     7681 atttaccaac ttatgaaaag ataatcaaat ttaatataag tttttcaaat aaaaagatac
     7741 caaattcttt ttcgagaatc tatataacat tacttaagct tggttggggt ataaacaatt
     7801 gagtagctac ttttgtatgg tgttcaactt tagtcaaaac tcttaagtaa tgcaaatgat
     7861 ttagattcat taatattcaa tctcttcata agtagtgaaa ttcgagttat ttctgcataa
     7921 ttatactatg atttatagat taattctatt agcatccaga ataatattgt ttatatgagt
     7981 aaaatttaaa gaatattgtg aaaattatag gatgttaaaa taaatttagg taaaaaagaa
     8041 gtgtttgtat attatagagt accttttcta aaatatgtgg gattgtttta agaattagaa
     8101 taaaaattat ttttaaatta gtatttttat aatgtatagt atttttaatg tataattaaa
     8161 ttgtgcagaa aatagaattt aaagtcctat agaatttctg attatggagg agaatatgaa
     8221 aagaaaagtt caagtaaaaa atattactat tggagaaggt agacctaaaa tttgtgtacc
     8281 aattattggg aaaaataaaa aagatattat aaaagaggct aaagagttaa aagatgcatg
     8341 tttggatatt atagagtggc gagttgattt ttttgagaat gtagaaaata ttaaagaagt
     8401 taaggaagtt ctctatgaat taagaagtta tatacatgat atccctttat tattcacatt
     8461 tagaagtgta gtagaagggg gagaaaaatt aatatcaaga gattattata ctactttgaa
     8521 taaagaaatt tctaatacag gtttagtaga tttaatagat gtagagttat ttatgggaga
     8581 tgaggtcata gatgaagtag ttaattttgc tcataaaaaa gaagtgaaag taattatatc
     8641 taatcatgat tttaataaaa ctccaaaaaa agaagagatt gtatctcgtt tatgtagaat
     8701 gcaggaattg ggcgcagatt tacccaaaat agctgttatg ccgcaaaatg aaaaagatgt
     8761 gttagtgcta cttgaagcta caaatgaaat gtttaaaatt tatgcagata gaccaataat
     8821 aacaatgtca atgtcaggaa tgggtgttat aagtagattg tgtggtgaaa tatttggttc
     8881 tgcattgact tttggagctg caaaaagtgt ttcagcacca gggcaaatat catttaaaga
     8941 gttaaattca gtacttaatc tattacataa gagtattaat taaattaatt atatagaaat
     9001 attagacggt atttttgaag atataaatta aatataaaaa acttttcaga tagtaaaatt
     9061 taaattgggc aattatatct gaaaagcaac tatttagtgt gtattttgaa agtgatatag
     9121 aaaatcttat aaatcaacta tggcatattt aaaatatgcc attaaaacct actagacatt
     9181 agattataga tggtatacta gcatttctat tatttataga tagtaagttt tcataataac
     9241 tgttttatct aaagtagaag atatatttgt attatgtata tttgtcatta tttttttgta
     9301 attatgtata ttaatggtta tatatgtaat ataatattat cactctattt tttgtatatt
     9361 gtacattaca acatttatat ctatcatcat caattacagt agaatgtatg ggatacttat
     9421 tttcacttaa ccaatatttt tgcatattct ttgtccaaga ctttggaaac ttcaatactt
     9481 tcttgtttat tataacatcc tcatcttcaa aaatattaac ttctctttct atttttattt
     9541 ttctgttgta tatcttattg atagattctt tgtcgagttt tatgtctcta tacatatcag
     9601 ggcaatagca acaaacataa tttctttttt ttcgacgttt aaatttcaca actttctcta
     9661 tgctaatttc taattttttt gtaccatctt ttataaattt aatatcttct atgttctcgc
     9721 ttgaaaaaat ttctgtcaat cttaaagtat ctacagtttc tctaattatt tgctttttat
     9781 aagaagagaa tccatatcca aaagaaaggt ttaatttatt agggatgtag ccatttttat
     9841 atttaaacat cttaattttt tccaattcag cattatcttt aaatttcaac ttttcatacc
     9901 taaaagttac tttgctaaaa agagtatatt ctacatcaat atattctttt gcaatcttaa
     9961 atttatcgct accttcaatc acaatcatag cctatctcca atcgttttat attaaaataa
    10021 ggccatagaa catggtctaa tagtactcat tagcactata agacaatgtt aacacaaaaa
    10081 gacaaaaaaa ggttatttat ttcaaataac cttttttttt atataaaata taaagaatta
    10141 tattttattc ttttttatga tgtctataat ctgtgatatc agatttagtt ctcttgaatt
    10201 taagtctttt aactcttcat ttatatcatt ttctaaatat gtttttccag tggaagtaat
    10261 atcttttata agaacatctg cagaaatatt taatatattt acaaatttta tgaatgtttt
    10321 aagacttgga ttctttttac cactttcgat ttgagttata taccaaggcg atactccaac
    10381 catatttgca aattcttttg aagttaaatt tgcttttttc cgttcttctt taagtctttt
    10441 acctatataa ttcattattg ttactatttc cctcacttat atttctttgt atttaatgat
    10501 gcactttatt aactctagca ttgcaatttg ttctttttca gttaatttat taatcatttc
    10561 ataacattct aaaggtacag agttatcaac gccttctgtt gtaataatat ttgcaaataa
    10621 caattcaagt ggtatgtcta aagcagtagc aattttcatt ataatattga tagatgtatt
    10681 tgtctcagct ctttcaatct gaccaatata tgttggatgt aaacctgcct tttctgctag
    10741 aaactcttga ctatatccca attttgttct atagactttt attctatctc ctattatttt
    10801 tcctatattt gccaattatt atccctccac tttagattat attcatagtt taagttgttt
    10861 tatatagact atttatactt ttttagtcta aatatagact atatatgttt ttatactgaa
    10921 ataagtgcta tttatttttg tatagtctgt atctacaata aacaaatatg gataaatcat
    10981 atttttaatt agagaaatat tttcataatt tcataaccat actacaataa taaaacgaat
    11041 taataatatg ttaattatag cattttttat tgtaattaca tattagatat gtagaa
//
LOCUS       DAYATN010000128        10910 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.128, whole genome shotgun sequence.
ACCESSION   DAYATN010000128 DAYATN010000000
VERSION     DAYATN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10910
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.128"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            59..793
                     /locus_tag="ACGI8A_003018"
     CDS             59..793
                     /locus_tag="ACGI8A_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181000.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            1004..2185
                     /locus_tag="ACGI8A_003019"
     CDS             1004..2185
                     /locus_tag="ACGI8A_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFH0181001.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVISDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            2169..2741
                     /gene="mobB"
                     /locus_tag="ACGI8A_003020"
     CDS             2169..2741
                     /gene="mobB"
                     /locus_tag="ACGI8A_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HFH0181002.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            2860..3135
                     /locus_tag="ACGI8A_003021"
     CDS             2860..3135
                     /locus_tag="ACGI8A_003021"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0181003.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            3378..5078
                     /locus_tag="ACGI8A_003022"
     CDS             3378..5078
                     /locus_tag="ACGI8A_003022"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181004.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            5441..6628
                     /locus_tag="ACGI8A_003023"
     CDS             5441..6628
                     /locus_tag="ACGI8A_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181005.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            6688..7851
                     /locus_tag="ACGI8A_003024"
     CDS             6688..7851
                     /locus_tag="ACGI8A_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0181006.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGDNPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            8002..8805
                     /gene="zupT"
                     /locus_tag="ACGI8A_003025"
     CDS             8002..8805
                     /gene="zupT"
                     /locus_tag="ACGI8A_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFH0181007.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            8909..9460
                     /locus_tag="ACGI8A_003026"
     CDS             8909..9460
                     /locus_tag="ACGI8A_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HFH0181008.1"
                     /translation="MEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGIFYF
                     HKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYKDIP
                     PKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVLQII
                     LLILYAVQLYRRLMYYLRLKKFK"
     gene            9710..10423
                     /gene="rgbR"
                     /locus_tag="ACGI8A_003027"
     CDS             9710..10423
                     /gene="rgbR"
                     /locus_tag="ACGI8A_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HFH0181009.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            10477..>10910
                     /locus_tag="ACGI8A_003028"
     CDS             10477..>10910
                     /locus_tag="ACGI8A_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFH0181010.1"
                     /translation="MNGILIYKKDSKKNEKIVFYIVLFLIYIIIQISRLFNGGNYFSN
                     INYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVYYKCLDAVIYRILFFAITGNV
                     ISSKESLLVPYLESIKIQIYSNLIIILIYLIFSFFRKSIKLI"
BASE COUNT         4295 a         1048 c         1943 g         3624 t
ORIGIN      
        1 taactgtaaa ttgagatata gataaatact ataaaattta agaaaggagg attgatttat
       61 gaagttaaaa aggtcattag tttgtgtaac tattttaggt attatacttg ttggatgtca
      121 taaagaaaat actaaagaga aaaatcaagt agcaagtaaa gcaacacagc aaaaaactat
      181 gacaaaagtt caaaatgatg ttaatgaaat tatgaataaa gattataagt acattataaa
      241 gaatatgggt ataccttata atacatttta ttatataaaa cctaaagttt taaaagaatc
      301 aaatacaatg caagatataa atacatcaag ttatatgaca ttagtttatc caaaatatac
      361 tggaaatgat gaactagatg gaagtgcttt atatgttgat attaacgaaa ataaagttgt
      421 caatgttgaa accaattctt tctcatcaca aggtatatca gtcattgatg ctgaatctag
      481 tatagtgatt gaaaaaagtg accatgaaaa atcggctgta tcactagaaa attttagaca
      541 tattgacctg ggagagtatg ttggtgttga agattccaga ataaatgaaa ttgtaggaga
      601 tgcgaattat gatttaacag catataatca tgaaggcagt aaagtagtta agagttatag
      661 attgaaagaa gataacaaaa tattaaaaaa agaggtactt acaataagta ttgtggataa
      721 caaaataaaa tctataaaaa ctattgaaag tgataaaatt gtaaaaataa taaaaggaac
      781 tttattagaa taaaatattc taaagcatat gatgctgttt caagatagaa ggaaatttct
      841 agctgggaac agttttttta ttagatttat aaaatttgaa ctgcacttat agataactat
      901 tagttataat taaatataag ataaaagtat ttccaacttt tataatctat aatatattac
      961 atagaaatat cttaaataaa aaatatattg gggagtattt aaaatgatag aattagaaga
     1021 tgccataaaa attattgaaa aaaatgtttt accaataaac agggtcaaga tagtaaatct
     1081 tatagatgca gtaaataagg tagttgcaga agatatatat tcaacaattg atagtcctcc
     1141 atttgatagg tcaccctatg atggttatgc atataatgca aattctgaaa atagaaattt
     1201 aaaggttata gggactttgt atgcaggaga tgtatttaat agggtattaa ataaaaatga
     1261 agcagtgaag ataatgactg gtggaaagat accacagggt gcaaattgtg taataaaaaa
     1321 agaagatgta acagttataa gtgatatgat aactttaaat ataaaattaa aggaatacga
     1381 taattacata tttaagggtg aggatataaa aaaggggcaa ttgattatta ataaaaatca
     1441 gaatatagga tatgatggta ttggagtatt agcaagtctt ggaatatcca aagtaactgt
     1501 ttatgatgat ttaaaagttg gaattttgaa tacaggtaca gaactacaag atataaatga
     1561 gactttagag aatggtaaaa tatacgatag taatagatat accttatatt caagattatt
     1621 aaaacttgga atacaagcag atacaatatg caattgttct gatgatatat tggagttaga
     1681 aaaaaaggta aagaatatgt tacagagtgt agatttttta attaccacag gtggtgtttc
     1741 tgttggagat aaagatttaa ttccagatgt attcaaaaaa ataggggcaa cagaattatt
     1801 ttggaagata aacattaaac ctggtggagc ttgttatgta ggtacattgg gagaaaagct
     1861 attatttggt ctttctggta atccacatgc agcaatagtg gtatttgata atgtgatagt
     1921 tcctgttatt gagtatttaa catataagaa gggaacacat catataaaag ctatatttaa
     1981 aggagaattt aataaacttt caaataaaga tagatttgta agtggaaaac tatatacaag
     2041 tgaaggtaaa ttgtatgtag agctaaatga taagaaagat gcaaaagcta gattatctgc
     2101 aactctaaga agtaattgta tgattaagat taaaaagaat gagacaataa aagaaaatca
     2161 attagtggat gttgttatta gatagtttta tgaaaggaca caattttatg tataatatat
     2221 tgtcagttgt ctcatattca ggagtaggta aaactacatt gattgaaggc ataataaaag
     2281 aattaaataa aaaagggtat cgtgttggag ttattaaaca tacctgtcat gattttgata
     2341 tggatgagga aggaaaagat acttataaac atagaaaatc tggggcgaga aaagtctgta
     2401 taatatcaga taaacgagta gcatatattg aagaactaaa agagaagaat ccattatata
     2461 aaatgattca attatatgaa gatatggact tgattatcat agaaggatat aaaaattata
     2521 gatttaaaag attggaagta actagaaaag gtaagtatga ggatatttta tctaataagt
     2581 ctgatttgat aggtattgtc agtgatatag aatataaatt gaatattgaa cagtttaatt
     2641 taaatgatta taaaaatata tcaaagtaca tagagtcttg tataaaaaat gaaattttaa
     2701 gtataagcaa tgttgaaata aaaaacatat taaaagaata attttaagat gttcttctta
     2761 tataaaatga tataattatt tatataaata aagatataag aaatttatca attttatttt
     2821 gagttataaa atattaaaga aattgtagga ggacaatata tgaaaaaaat attagtagcc
     2881 tgtggagcag gaatcgcaac atcaacaata gtttgtgaca gagtagaaag attggttaaa
     2941 gaaaataata tacaagcaga agttgagcaa tgtaaaattg ctgaatgttc aacaaaacaa
     3001 gaaggtgctg atttaatcgt atctactact atactaccaa caacttatga tatacctaca
     3061 ataaaggcaa caggatatat aacaggtata aatacaacag cattagataa aaagatatta
     3121 aatgcattga agtagttttg tatttataaa aaaaatttag tattaaatat agagttgtat
     3181 ctttataaaa ttttaaatat agagatatag ctctttttat gttgtttcaa gtatacaact
     3241 atatgatata aatcttcaat tttatgaaaa agattgttat tactttgtaa gtttataaaa
     3301 gatttaaagt tattgttatg ttatactaat ttatgggtat tttttagaag tactaataaa
     3361 ggagggattt aatacttatg ataaaacaat ttgtcaaata ttataagcca tataagaaaa
     3421 tttttacact agaccttata gcagcttttc tattttcctt atgtgattta gtttatccta
     3481 tgataactag aaatataatg gatgacgttg ttccaaataa aaacttaaga atgttagtag
     3541 tttttgcagt agcacttata cttatattta tagcaaaagc agggcttaac tattttatgc
     3601 agtattgggg acatgttatt ggtgtagata tgcaagctga tatgagaaat gaggtattta
     3661 cacacttaca aagacttcca aatacgtatt ttgataataa caaatctgga gtaactatgt
     3721 ctagaatagt aaatgattta atggatataa cagaacttgc acatcatggg cctgaagatt
     3781 tattcatttc tatagtaatg ttggttggtt cattctttat acttatagat ataaatattc
     3841 ctcttacgct tataatattt gctatattac cgtttataat ttggtttgca atagctaaaa
     3901 aagataagat gaatattgct tttatgaaga gtagagttac tataggtgat gtaaatgcta
     3961 ctttagaaaa tagtatagca ggtatgaagg taactaagtc tttttgtact gagaaagaag
     4021 agttaaataa gtttgtaaga agcaataaat tatttagaag agcaagacaa gattcttata
     4081 aggtaatggc agaatattac tctggtatga atttatacat ggatatctta gagtgggtag
     4141 ttgtaatagc tggtggttat tttacttata taggaaagat tactttgggt gattttgctg
     4201 cttatatact atatgtaaaa atgtttatac aacctatgaa aaaattaata aactttacag
     4261 agcaatatca aaatggtatg acaggtttta aaagattcat agagataatg gaacaagacc
     4321 atcaaaaaga agctaaaaat cctattgaac ttgaaaatgt aaaaggtgac attgagatag
     4381 aaaatatatc tttcacatat gaagataaga ctcaagtttt ggataattta agtttatcta
     4441 taaaagctgg taagactata gcattagttg gaccttctgg aggaggaaag actacattat
     4501 gtaatctatt gccaagattt tatgaatttg ataagggtga tataaaaatt gatggaaaga
     4561 gtataaaaga tgttagttta aaatcattga gaaaaaatat aggtatagtt caacaagatg
     4621 tatttttatt tacaggaact ataagagata atattctttg tgggaaccct aatgctacag
     4681 atgaagaaat gatagctgct gctaaaaaag ctagaataca tgactttgta gagactttgc
     4741 cagatggata tgatacttat ataggagaaa gaggcgtaaa attatcaggt gggcaaaaac
     4801 aaagaatttc aatttcaaga atctttttaa agaatcctcc aataataata ttggatgagg
     4861 ctacatctgc tttagacaat gtaacagaaa gagaaattca agagtcttta gaggaattaa
     4921 gtaaagatag aacaaattta gttgttgcac atagattgac tactataaaa aatgctgatg
     4981 aaatcatagt attgacggat aaaggaattg aagaaagagg tactcatgaa gagctagtaa
     5041 acaaaaatgg agtatatagt aggttacaca ataactagta tacaaatttt acaccatagc
     5101 taacatacac attaaactta caaacatagg atatattaag attcataaag acaattcatt
     5161 ttatatttat aagatgtttt atgaataaaa tgtatcctat gttttatttt ttgcaaatta
     5221 catttaattc tctttgtaag aatagagtta aatagccaat ggtcgggata acgtttttac
     5281 aaaaagaata atttagaaaa ctaactttaa tttatggaat ttatcgaatt ttatgaaaaa
     5341 aatcgaattt tgacttgaaa tgtttttcaa aagtagtaaa ataatggata actatacata
     5401 cattttataa aaatatattt attatgaggg gggattttgt atggaggcaa taacagcttt
     5461 ttgtctagtt ttagtatcac tgacgatagg agatatagtt tcagcaaaaa ctaaagcttt
     5521 tgttccgtcg gtttttgtat cagcaattat atttatagta ggattttgga cttttttccc
     5581 agaaaatatt gtggacttag ccgctttagg aacaccatta gctcaattag gtatgctttt
     5641 gttaataact catatgggaa ctatgatgag tattaaggaa ttagcaggtc aatggaaaac
     5701 aatagtaata gccttggcag gaatagtagg aatatgtgta ggtgctttgg cattagggac
     5761 agtagtattt ggatgggata cagcagtaat agctacacct cctcttactg gagggctagt
     5821 tgcatctata atgatggcag atgctgctac agcaaaaggt cttgcaagtt tatcagtact
     5881 tgcaattttg atgtatgttg cacaaggatt tgcgggttat cctattacag cattaatgct
     5941 taaaaaagaa ggaaaaagac ttttatcaga ctttagaagt ggaaaagtaa ctgtaaatac
     6001 agatgaagaa aaagttaagg atttacctga acaaaaaagc agatttaaga taataccaga
     6061 cttaccagaa aaatatgata cgacatatat gataatactt agattaggat ttgtagcatg
     6121 gttagcagta caatttacaa atcttacaaa tgaagttgta tcaagatatg ttatatgttt
     6181 aatatttggt gtaatagcat cagaacttgg aatagttgat agaaaaccac ttaataaatc
     6241 tggttcattt ggatggttaa tgacatcatt gatggctttt atatttgcag ggttagcaaa
     6301 agctacacca gcaatgcttg ttgaaatggc agttcctctt gtaggaatca taatatttgg
     6361 tgtaataggt atgggaattt tctctattat agtaggtaag cttttaggtg aaagtaaaga
     6421 aatggctttt tcagtttcgc ttacatcact ttatggtttt ccaccaaact atatattaac
     6481 tgaagaggca gtaaaggctt tggctgaaac accagaagaa aaagaatttt taatggataa
     6541 aatgcttcca aagatgcttg ttggaggatt tactacagtt acaatagctt ctgtagtaat
     6601 agcaggtatc tttgtaaatc taatatagta tttaattgat aaaaagtatt ttaaataaga
     6661 agaatgtgta ctacttggag gtatagaatg gatattaaag aaataacaaa gagttataaa
     6721 gactatgtaa ttaagcttag aagggaattt catgaaaatc ctgagaaaag tatggaagaa
     6781 gttagaacat caaagagagt taaagaagaa cttgataaaa tagggatacc atatgtatca
     6841 gctggaggta caggtgtaat tgcaactata aaaggagata atccaggaaa gactgtagca
     6901 cttaggggtg atatggatgc tctacaagtt gttgaatgca ctgatgtaga atacaaatct
     6961 aaaaatgaag gacttatgca tgcttgtggt catgatggtc acacatcaat gcttctaggg
     7021 gcagcaaaag tgttaaatga tattaaggat agtatcaatg gaactgtaaa attatttttt
     7081 cagccaggag aagaagttgg taaaggtgca agagctatga ttcaagatgg tgctatggaa
     7141 ggtgtagata gtgtgtttgg aatacatctt tggactgatg tagaatctgg gaccatatcg
     7201 gtagaagaag gaccaagaat ggcttctgct gatttcttta aaattactgt aaaaggtaga
     7261 ggtggtcatg gttcacttcc tcatcaaggt gttgatgcag tgcttgcaag ttcagcaata
     7321 gttatgaatc tacaatctat ggttagtaga gaagtaagtc ctctggaacc attagttgtg
     7381 agtgttggag ttttaaattc tggaactaga tttaatgtaa ttgcgagtga agctatctta
     7441 gaaggtacaa taaggctatt taatccagag cttagaaagc aaattcctgg aattttagaa
     7501 cgtatagcaa aatctacagc agaagcatat agagcagatg ctgaattaga gtatgggtac
     7561 ttaacaccag cagttataaa tgacaaagaa tgttcaaaga tagctacaga tgcagcaatt
     7621 aagctatttg gagaagactg tataacacta tttgaaaaag taacaggagc agaagattta
     7681 gctgagttta tgaatatagc acctggtgca ctagcatttg taggagctag aaatgagtca
     7741 aaaggtgctt gttatcctca tcatcatgga tgctttaata tagatgaaga tgctctagaa
     7801 attggaactg ctttatatgt gcaatatgca gtggatttct taaataaata attttaatat
     7861 actataaaga aaaagcagag actaagttta tatctctgct tttttattgt gtatattttt
     7921 tataaatatt gactatataa aatatatatg atagcataaa gaaaatgata atcaatatca
     7981 attaaaaaga aggtgattta tttgaataat gtaatatttg catttttgat aacattattg
     8041 gctggtttat ctactggtat aggaagttgt atagcatttt ttgctaaaaa aacaaatact
     8101 aagtttcttt ctataagctt aggattttct gctggtgtta tgatttatgt ttctatgata
     8161 gaaatttttc ctaaagcaca agatgctttg acaaaatcta tgggagagaa attaggaagt
     8221 tggtcaactg taatagcttt ttttataggt atggctatca ttgctgttat tgataagcta
     8281 attccacaag aagaaaatcc acatgaaata aagaaaatgg agaatataga agaaaagaat
     8341 atcaaaaaaa ataagtctct tttaagaaca ggtatattta cagcaatggc tatagcaatt
     8401 cacaattttc ctgaaggatt agctacattt atatcagctc ttgatgatgt tacaatagct
     8461 attccgatag caattgcaat tgcaatacac aatataccag aaggtatatc tgtatctgta
     8521 ccagtttatt atgccacagg agataaaaag aaagcttttt attattcctt tttatctggt
     8581 atgtctgagc ctttaggagc gattattgga tatgtacttc ttagaaattt tttaaatgat
     8641 ataactcttg gaattgtatt tgcaattgta ggaggaatta tggtattcat atcactagat
     8701 gaattattgc catcagctag agagtacggg gaacatcatt tatctattta tggattgatt
     8761 gcaggaatgg gtgttatggc tattagttta ttactattta aataactttt aaatatatga
     8821 ttgtaaccta aaattagtat caaaatgata taattattta aatagatttt taagctattt
     8881 aaaaatatca ttttggagaa tgagtgctat ggaaaagaaa ataaaattca aatttgactg
     8941 gtatagttca cctgataaga tagagaaatg gttagaagat attgaagcta aaggatataa
     9001 tttatataag ataaataaat ttggaggaat attttatttt cataagggaa gtccaagaaa
     9061 aataaaatac actgttgatt actactatga tgtagagaat aattattttg atgaacataa
     9121 atcaaatggc tggaatcttg tatttaggag tattggtagt tttatgggta catctgcatg
     9181 gtatatatgg tcgaaagaat acaaagacat acctcctaaa ttatatagtg attctgataa
     9241 aacaaataaa gtaaagtcaa ttttatttat gaatatgttt atattcttta tgttagtagt
     9301 aatatttata tatttattga atattgaaat aagaaattta tatagtggtt atggagcatc
     9361 tgtaactgct attgtactac agattatatt gttgatttta tatgcagtac aattatatag
     9421 gagattaatg tattatttaa ggcttaaaaa gtttaaataa ataaaagaag gtgtcctaaa
     9481 attatttttt taatatttta agacatcttc ttttgtcagt aagtactgtt tttaattata
     9541 gtaatttctg tctttaaatt gaaatatcaa ataatagttg tatgggattg agtattgata
     9601 aaaataattt aaaaatgtta tcctaaagta tcagatatac aaaattatct ttaaatatat
     9661 tataaatatt ttaattgtat taactagaaa ttatgtaagg aggcatttta tgattaagat
     9721 agcagtttgt gaagatgaaa aagaaacaca acttttaata gaagattacc ttgagaatat
     9781 attaaaagat ataagtatag aatatgagat acaaaagtat atatctggag aagagttact
     9841 ggaaagtaat ttaaaagata tagatatatt acttcttgat ataaaaatgg aaaaattaaa
     9901 tggaatggat actgcaagaa agattagaga agtagacaat gaaatggaaa taatatttgt
     9961 aacttcatta atagattatg tgcaagaagg ttatgaggta agagcttata ggtatttact
    10021 aaagccaata gagttggaag aactaaaaaa acatgtgcta acttgtatca aagatattga
    10081 aataaataag gaaagtcata ttacaataaa aaataagtct aatacatata agatttattt
    10141 aaatgaaata aaatatatag aagttcaaaa aaaagatatg ctaatacaca caataaacaa
    10201 gaattttgat ataaaatata gtttaggcaa aatagaaaaa gaattaaatc catataaatt
    10261 tataagatgc cataaaagtt ttatagtaaa tttaagatat gttgaaaata taaagcctaa
    10321 tactgcaata ctggaaagtg gagaagaggt acctattagt agatatagat ataaagaagt
    10381 taaggagaaa ttcttaaaat ttcttggtga tacaatatgc taaactttat gactgagcta
    10441 ttagtagttt tatctatatt tatacagttt tctataatga acggaatttt aatttataaa
    10501 aaggatagta aaaaaaatga aaaaatagta ttctatattg tcttgtttct tatttatatt
    10561 ataattcaga taagtagatt atttaatgga ggaaattact ttagtaatat aaactattta
    10621 ttttatatat tagcatttat tgcttgggga ggatactata attattttta taagatgggt
    10681 tatattaagt attccatttt cttttatcta tttagtgtat attataaatg tttggatgct
    10741 gttatatata gaatactatt ttttgcaatt acaggtaatg ttatttcatc taaagaatct
    10801 ttattagtac cttacttaga atctataaaa attcagatat atagtaattt aataattatt
    10861 cttatatatc ttatatttag cttttttaga aagtctataa aattaattag
//
LOCUS       DAYATN010000129        10893 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.129, whole genome shotgun sequence.
ACCESSION   DAYATN010000129 DAYATN010000000
VERSION     DAYATN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.129"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            235..699
                     /locus_tag="ACGI8A_003029"
     CDS             235..699
                     /locus_tag="ACGI8A_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFH0181011.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            706..1200
                     /locus_tag="ACGI8A_003030"
     CDS             706..1200
                     /locus_tag="ACGI8A_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HFH0181012.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1216..2241
                     /locus_tag="ACGI8A_003031"
     CDS             1216..2241
                     /locus_tag="ACGI8A_003031"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HFH0181013.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2228..4675
                     /locus_tag="ACGI8A_003032"
     CDS             2228..4675
                     /locus_tag="ACGI8A_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFH0181014.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNKVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4855..6228
                     /gene="radA"
                     /locus_tag="ACGI8A_003033"
     CDS             4855..6228
                     /gene="radA"
                     /locus_tag="ACGI8A_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFH0181015.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSEKLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6231..7301
                     /gene="disA"
                     /locus_tag="ACGI8A_003034"
     CDS             6231..7301
                     /gene="disA"
                     /locus_tag="ACGI8A_003034"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HFH0181016.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7390..8484
                     /locus_tag="ACGI8A_003035"
     CDS             7390..8484
                     /locus_tag="ACGI8A_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFH0181017.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8613..10790
                     /locus_tag="ACGI8A_003036"
     CDS             8613..10790
                     /locus_tag="ACGI8A_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181018.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         4563 a         1098 c         2012 g         3220 t
ORIGIN      
        1 atttaaaata taaagtattt aaagttctga aatctaatca tgaagcaaaa aattagcatt
       61 tggttatcaa taaatataat aaataaaaac tcataattaa tgaaagaata aattcatatc
      121 taaatttcta taaaaaattt aaatttatac aatttagggc tttaaaaatt taaataatag
      181 gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt gtgtggtgat attaatggca
      241 actatgacag atataattga aaaatttatt aaagatttga tggaagaaga taatagcata
      301 caaattcaaa gaaatgaact agcaaacttg tttagttgtg caccatcaca aataaattat
      361 gtcttaacaa caagatttac aatagataga ggatattaca ttgaaagtaa aaaaggtggc
      421 ggaggatatg ttcaaataga aaaaatacga aaaagtaaag atgggcatat tagagaacta
      481 ctaaatgaaa aaataggaag tcagatatcc tataaaaaag caaaagaatt attagaaggt
      541 ttaaaagagt cggatttgat taacgaaaga gaattaaaat taatattata tgcaatagat
      601 gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga aggtaaggtc taatatttta
      661 aagaatataa taataggatt atttagtata gaggagtgat aatttatgct atgtcaaaaa
      721 tgcaataaga ataaagcatc tgtatattat aataagatag tcaatggaga aaaaacagag
      781 atgtatttat gtagtgagtg tgcaaaagag aatacagaaa tgaattttaa cttagatatg
      841 cctttttcaa tgatggatat attttctaat ttaggttttc aacctaaaaa agaacttgag
      901 gaaaagctag tttgtcctaa atgcaataca acatatagtg agtttaagaa taatgggaga
      961 tttggatgta gtgaatgtta taatgcattt agtagtcaag taaatccaat gcttcaaaat
     1021 atacatgggc atatagaaca tacaggaaaa gcacctaaaa aatcttttta taagataagt
     1081 gtagaaaatg agattaaaga actaaaagaa gatttagata gagctataaa aaatgaagag
     1141 tatgagttag cagctcaatt tagagataaa attaaatatt taaaaggttc tattgactaa
     1201 gattggagtg atattatgaa agagaacata gtcatgaaat caagagttag gctagctaga
     1261 aatttaaata actatccatt tccaaataaa ttagataaag aatgtgccat ggaaataata
     1321 gaaaaagtaa aaaatgcgtt tattaattct aacttagaac aaaaagaaga atttgatttc
     1381 tataaaattg aagatttaga ccaatctaaa aaaatgctta tggttgaaga acatataata
     1441 agcccagatt tagcagaaaa tgataaaagt gcagttatag taaaaaaaga taaaactata
     1501 agtataatga taaatgaaga agaccatata aggatacaaa ctatatgtga tgatttaaac
     1561 ttagaatatg catatagtgt agcaaatgaa atagatgatt tgttagaatc aagtttagaa
     1621 tatgccttta acacaaagct aggttatcta acatcttgtc caactaatac aggaacagga
     1681 atgagagctt ctgtaatgat gcatttacca gcattaagtc aactaggcta tatggatgaa
     1741 ctttataaaa tttcatcaca aataggtata gcaattagag ggatatatgg agaacgaaca
     1801 gaagcgttgg gaaatatata tcaaatatct aatcaactta cacttggaag aacagagtct
     1861 aatattatag aaaatgtaag tggacttaca aaagatgcaa tttcaaaaga aataaaagct
     1921 agagaaatat tgcaaaaaaa attaggtata aaactagaag ataaaatatt tagatcgata
     1981 gggacacttg aaaattcaag agtaatgagt agcgcagagg caatgagcca tctatcaaat
     2041 ataaaaatgg gaatagaaat gaactatata gataaactag atttaagggc tatagaacaa
     2101 cttatgatag gtatacaacc agcgcatcag tcaataatgt ataagagtga tgatgttgag
     2161 aatagagata taaatagagc aacatatatt agagaaactt tggaaaaatt aagaggaggg
     2221 atgaattgtg aacttcaata gatttacaca aagagctaaa aaggcaattg atttagcgtt
     2281 tgaatctgct aaaagtctag gacacaatat tgtcggaagt gaacatatac ttttaggtct
     2341 tttaagagaa gaagaaggta tagctgctaa agttctaagt aaagttggat ttacagaagc
     2401 ttatttagaa ggcaaaatag ttgatatgga aggtaaggga gaagaaatct cagaagatat
     2461 agtattaagt ccaaggagta aacaaatact tgaattatca ggaatgtttg caaataaatt
     2521 aaaaacaaat tatattggga ctgagcatat attattagca attattcaag aaggtgaagg
     2581 aatagctaat aagattttaa attatgctgg agtaaatgat aggactttag cccaattaac
     2641 aattgatatg atgggtatta gtgataaaaa tcaatacaaa gcagagaata gttacacggg
     2701 caaccagaat caagcagaat ctaaagtact tgataagtat ggaagaaatc ttacactata
     2761 tgcaaaacag aataaaatag accctgtaat aggtagagaa aaagaaatac aaagagtaat
     2821 acaaatatta agtagaagaa ctaaaaacaa tccagtgttg ataggagacc caggtgtagg
     2881 gaaaacagcc atagctgaag gtctggctac aaatatagcc ttaggaaatg ttccagaaac
     2941 actaaaaaac aaaactttat actctttaga gatgggttca ttattggctg gagctaagta
     3001 tagaggagaa tttgaagaaa gaattaaaga agttgtagat gaagttgtta aaaatggaaa
     3061 cataatctta tttatagatg agatgcacac aataataggg gctgggtcta ctggagaagg
     3121 ctctatagat gcttcaaata tattaaagcc agccttagca agaggagaaa tacaagttat
     3181 aggtgcaact acaattgatg aatatagaaa gcatgttgaa aaagattctg ctctagaaag
     3241 aagattccaa ccagttatgg tagatgaacc aactaaagag gactcaataa aaatattaga
     3301 aggattaaga gataaatatg aagctcatca taaagttaaa ataactgatg atgctataaa
     3361 ggcagctgta gaattatcaa ctagatatat atcagacaga tatctgccag ataaagctat
     3421 agacttgatt gatgaagctg catcaaaagt aagattaaaa gaaaatacac ctccttcaga
     3481 aataaaaaaa ttagaactag aaatagaaaa tatagataaa gaaaaagaag aagcagtaag
     3541 atgtcaagac tttgagaaag cagccaaaat aagagatgaa caaggtctac ttaaaaaaca
     3601 attagaagat gttagagaaa gatggaacaa gtcatctaaa cattcagatt tagttgatgg
     3661 agaagttatt gctgaagtag tgggcttatg gacaggaata ccagttaata aaattcttga
     3721 agaagaggct gatagacttt tgagacttga ggaaatactg cacaataaag ttataggtca
     3781 agaacaagca gtaaaatcta tttcaaaggc aatcagaagg tcaagagcag gtcttaaaga
     3841 ccctaataga ccaattggtt catttttatt tttaggtcca acaggagtag gtaaaacaga
     3901 attatctaag gcattagcag aagtgcaatt tggagatgaa aatcaaataa ttagaataga
     3961 tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg ataggttcac ctccaggtta
     4021 tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta agaagaaatc catattcagt
     4081 tatcttattt gatgagatag aaaaagctca tccagatgta tttaatattc tgttacaaat
     4141 tctagatgat ggtagactaa ctgattcaaa aggaagaact gtagatttta agaatactat
     4201 tgtaataatg acatcaaatg ttggagcatc tacaattggt agacaaaaaa ctttaggatt
     4261 tagtatagct aaaggagacg aagaagaaaa atctcaatat gaaaaaatga aagaaaatat
     4321 aatgggtgaa ttaaaacaaa gattcagacc agagttttta aacagaatag atgatataat
     4381 tgttttccac tcactaaatg aaaatcatat atcaaaaata gtacttttaa tggcagcgaa
     4441 actacaagag agattaaaag aaatggatat aaaattagaa atgagtgatg aggctgttaa
     4501 attaatttct aaatctggat ttgacttaga atatggagca agacctctta aaagagcttt
     4561 acaaaaagag ttggaagatg agctatctga agcaatctta aaaggtaatg tgaaaaaggg
     4621 aagtaatgta gtagcaaaag tcaaagatga aaaaatagtt tttgaaacta aataatttaa
     4681 tagagttgca ttttaaaata ttgcaaatat aagttttata atctaaacaa aattttaagg
     4741 gcaatgaatc tataataatt cattgtcctt aattatttta tttaatcttt acatattgtc
     4801 tttcagatat gctaaaatta tagtatagaa taaaaaatta aggatgataa aagtatggca
     4861 aaaataaaaa caaaatatgt atgtcaatct tgcggatatg aaacagctaa atggcttggt
     4921 aaatgtccag aatgtacaaa atggaataca tttgtagaag aaatagacca aaaaagcacc
     4981 aaaaaagagg tctttataat agataaatct tcatctaaac cagtcagtat aaactcaata
     5041 gagagtaaag aagaagagcg gtttacaaca gatataaatg aattagatag agtgcttgga
     5101 ggaggtatag ttaaaggctc tttagtgctt gttggtggtg accctggtat aggtaaatca
     5161 acacttttaa tccaagtatc tagtaatgtt gccaatttag gaaaaactgt tttatatata
     5221 actggagaag aatcagaatc tcaaataaaa atgagagcta aaagattagg tattaactca
     5281 gaaaaattat atatatttgc tgaaaataat ttaagtataa ttgaatctta tttagaaagt
     5341 gtaaatccag aattaataat tctagactca attcaaactg tcttcagtcc agaaatatcc
     5401 tcggcaccag gaactgtcag ccaaattaaa gaaggtactt caaaattcat gaaaatttct
     5461 aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta caaaagaagg ttcactagca
     5521 ggaccaaaat tattagagca tatggttgat actgttctat attttgaagg agaaagatat
     5581 aatacttata gattagtaag agctgttaaa aatcgatttg gttcaactaa tgaacttgga
     5641 gtatttgaaa tgagggattt aggattagta gaacttgata atccatctaa aattttaatc
     5701 tctgaaaaac caaaagatgt agcaggttca gtaataatat ctactgtaga aggtacgagg
     5761 ccaatgttac ttgaattgca agcattagtt tctcctacaa gttttggtat accaaaaaga
     5821 acatctacag gagtcgatta caatagagta ggtatgttat tagcagtatt agaaaaacgt
     5881 gttggtttgc aaatacaaaa tcaagatgtc tatataaaca tagttggagg aataaagata
     5941 aatgaaccct caattgattt agggatagcc atttctgtag catctagttt tagaaatata
     6001 ccgatagatg aagatatagc tgttacagga gaagtgggac ttacaggaga agttagagca
     6061 gtaagcttta tagaaaagag aatagctgag tgtaaaaaac ttggatttaa aaagatagta
     6121 gttcctagaa gtaattatga tgttgtaaaa gagaccaaag gcatagaaat atggccagta
     6181 gataacttaa gacaagctat aaatatagta cttgggagga atcaataaat atggagaatt
     6241 ttctagataa taaaaatatg ctatatgcat taaaaatgat atctcctgga actccactta
     6301 gattaggtct aaacaatgta ctaagagcta agactggtgg attaattgta attgcaacaa
     6361 acgaagatgt aatgaaaata gtagatggag gatttgctat aaatgcagaa tattcaccat
     6421 catatctata tgaattagct aaaatggatg gagctatagt tttaagtggt gatgtaaaga
     6481 aaatattatt tgctaatgca caacttatac ctgactattt tatagaaaca tcagagacag
     6541 gaacaagaca tagaacagca gaaagagtag caaaacaaac tggtgctata gtcataggaa
     6601 tttcacaaag aagaaatgtt ataacagttt atagaggaaa tgagaagtat gtagtcgaag
     6661 atatatctaa gatatttact aaggcaaatc aggctataca aactctggaa aaatataaga
     6721 cagtattgga ccaagctgta acaaatttaa atgccttaga gtttaatgat ttggtaacta
     6781 tttatgatgt tgcattagtc atgcaaaaga tggaaatggt aatgagagtt acaagtataa
     6841 ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt agtaagtatg caattagaag
     6901 aattaatggg tacaaccaga atagaccaga aattaatatt caaagattat aataaagaaa
     6961 acacagaaat aaaagaactt atgaaaaagg tcaaaaattt aaattcagaa gaactaatag
     7021 aattggtcaa tatggcaaaa ctattagggt atagtggttt ttcagaaagt atggatatgc
     7081 ctataaaaac aagaggttat aggattctta gcaaaataca tagactacca acagcaataa
     7141 tagaaaactt agtaaattat tttgaaaact ttcaacaaat tttagatgca tctattgaag
     7201 aattagatga ggttgaagga ataggtgaaa taagagcaac atatataaaa aatggactca
     7261 taaaaatgaa acaattagtc ttattagata gacacatatg atgaatttta aatgaatttt
     7321 actacttttt tcttgaatat aatatacact ttggtctata atagataagt ataactaggg
     7381 ggtgaagcat tgataaggaa agtaacaaga atattgttta ttttacttgg atttacaata
     7441 ggtataacaa cctatttaac attaatgaaa gattttgaaa tattaacatt tggaaaagaa
     7501 acatatggtt atatcgcagc tgtagtagca ggtataataa tagccatttt aggatatctt
     7561 atagagccat gggttgtaaa taaagtaaaa gaaattgcta aaatagtgga taaagaattg
     7621 tcaaaatatc ctcagactga tatattgtta gggtctatgg gtcttatagt aggatttgtt
     7681 attgcgtatt tattaagtgg attagtaaat agaataccaa tagtaggtgg aatactatcc
     7741 ttactattat atttattcct aggatatctt ggaatgaaag ttgcgcttaa aagtaaaaat
     7801 gatttattta atattggaaa actaggaaga ttagcaaatc ctattaagga taaagataag
     7861 gataaagaaa ataaaaaaga agtaaaagcc attcctccaa aagtattaga tacaagtgta
     7921 ataattgatg gaagaatagc tgatatttgt aagactgggt ttatagaagg aaaacttatt
     7981 attcctgctt ttgttttgga ggaactaaga catattgcag attcttcaga tgatttaaaa
     8041 agagtaagag gtagaagagg tctggatata ttaaatataa ttcagaaaga attaaatata
     8101 gaagttgaaa taagtgagag agactttgat gatatagcag aagttgatag taaattatta
     8161 aagcttgctc aggttttaaa tggaaaagtt gttacaaatg attataactt aaataaagtt
     8221 gctcaattcc aaggtgttga agtattaaat ataaatgagt tagctaatgc tataaaacca
     8281 gttgctatac caggtgaaga tatggttgtt caagtggtta aagaaggaaa agaggctcaa
     8341 caaggtgttg catatctaga tgatggaact atgatagttg ttgatggagg aagaaaatat
     8401 atgaatgaga ctataaaagt actcgttact tctgttcttc aaactcctgc aggtagaatg
     8461 atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc tataatttgg gctgtttatt
     8521 ttaaatagat ggtccaaatt tatttattta taacgataat gtttaatgta ttcttaaaga
     8581 tggggattta aatcagaaag cagggagaat ttatgagaaa aaagattata tctttcttag
     8641 caacatttat cataatatta acatcagcaa gtcagtatag ttttgcagat gatatatcaa
     8701 cacgtggaaa agtaatattt atagatatga atagaactag tatgagtaat atgttaagaa
     8761 taaaatcatt aagagaagaa ctagacaata gagggtatat tggcttgatg aatataagag
     8821 gcgataaagg aagtgatgat agaaggtcat atgctagtat gggtgctggt ggtagagcta
     8881 atgtggctaa tgaagaagat attaattttg aaagctcgag taaggataga aatatagttt
     8941 ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa tttaactata aataagtcta
     9001 taaatgaaaa tttaaacttt ggagagtatg gttctgtatt agggtcatta ggacaaagtt
     9061 tatctgataa tggattaaaa gcatctgtat tgggtaattc agatataatt gaaaatggtc
     9121 aacttataaa aaatagaaac ttatgtctaa ctgcaatgga tgaatatgga agaataccta
     9181 atggaaatgt agatactata aataaaaaag acttatctat gccttatgga atatcaactg
     9241 attatgataa attgattgta gagactaaag aagcttacaa aaataatgat gtgatttttg
     9301 tggagcttgg agacacatat agattagatt tatataaacc aaatttaaat gaaaaaacat
     9361 atgagagtat gaaagataac atagaaaaaa atatagatat atatttaaaa aatatatttt
     9421 ctatggttga agaaaatgac actgtctaca tagcaagtgc atttccaagt gatttagatt
     9481 ataaaaataa aagaagactt tcgccaataa taaaattaaa tggagaaggg aaaggtatat
     9541 tatcatcttc aactactaga agagaaggta ttgtaacaaa tttagatgtt ggggttgaaa
     9601 tattagacaa ctttaatata aaaaatcaaa atatggttgg aagaaaatat gaacttatta
     9661 acagagatga taataaagaa tttttgatgg atgaatatca aaagatagta tctatttcaa
     9721 gcataaggtc tacaatactg aatggttttg taagtatagt tttcttgtca tggatagttg
     9781 ctatgattgc aattttattt agaaagcata tatcaaaaaa ttataaagaa acaacatttt
     9841 ttatattaaa ggaactttta aaaataggaa tagtaatgcc actgtcattt atgataacac
     9901 caataatgaa tttcaaaaca ccattagcaa tatctttagg aataattata ataacactta
     9961 ctatatatct tataagtaaa gttttaataa agaacgattt aaaaaacatg ctgtttttta
    10021 ctggattaac catagtaatt atggttattg atgctgggtt tggctcatat ttaatgaaaa
    10081 gcaatgttat gagttatgat tgtataattg gagctagata ttatggagta ggaaatgagt
    10141 atcaaggtgt ggcaatcggt agtgcaatat ttacatttgc aattttactt acatataaaa
    10201 atataccaaa atggtctata atagtttttt ctttggttat cttaattaca agtgcatctc
    10261 caattatggg agcaaatgtt ggaagtgcta tttctgaatg tgttgcattt ttactattta
    10321 tactattgat atataatgta aagatagatt tcaaaaaaat cgtattatta ggtatagcag
    10381 tagtatttgt attaggagtt tttgtagcaa tagatatgat tttgggaagt aattcacatt
    10441 taggcatgtt tgtaaaagaa atttacttta atggaccagg agaaataata caaacattta
    10501 gtagaaagat agagatgaat ttaaagctag ctcaaaccag tgcatgggta aatatactgt
    10561 taactggaat aggtgtaatt ttagttctta tgattaatca gattagatac tttaagcaac
    10621 tgatggatga gtatccaatt gtatttaaag gatttatagc atcaatagca ggatgtttag
    10681 taactctttt agtgaatgac tctgggattg tgtcatcagc tacagccttt atttatgtaa
    10741 ttgtacctat gataacatta agtataaatc ttacagcatt aaaagaataa gtactgtatg
    10801 aataatatgt aaatattaat acattaattt aacttcttta tttacaaaat aaagaagaaa
    10861 gtttataaaa aataaattat aataaaaaag agt
//
LOCUS       DAYATN010000130        10860 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.130, whole genome shotgun sequence.
ACCESSION   DAYATN010000130 DAYATN010000000
VERSION     DAYATN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.130"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            162..551
                     /locus_tag="ACGI8A_003037"
     CDS             162..551
                     /locus_tag="ACGI8A_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0181019.1"
                     /translation="MKQQEVVNKLRRGELMLMEYLWRKNSILSKKEIIEAMKEKYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNAIKEDRKVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKE"
     gene            645..1028
                     /locus_tag="ACGI8A_003038"
     CDS             645..1028
                     /locus_tag="ACGI8A_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0181020.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDGNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEENLSDDKLDMLEEWVKNWEEDE"
     gene            1746..3137
                     /locus_tag="ACGI8A_003039"
     CDS             1746..3137
                     /locus_tag="ACGI8A_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0181021.1"
                     /translation="MRNKIIIGITFLLVLLMSGCASKQTAEINVLATTDLHGVIPYEL
                     TSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGDNNTESYIETPISK
                     DMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTYKKNGDSYTKPYII
                     KNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVE
                     DAKKWVSIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQ
                     IEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDKSSKLIKFEKNQES
                     DKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDIYKTIGYKWRNISS
                     PKSDGMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKDNVVTIYPNKNDKFKIEK
                     GKQEYSINLKHIQ"
     gene            complement(3285..3593)
                     /locus_tag="ACGI8A_003040"
     CDS             complement(3285..3593)
                     /locus_tag="ACGI8A_003040"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0181022.1"
                     /translation="MLREQGSAIRDTLDSVLSLSNQKAYPVWESVNSFALIKAAQAGL
                     GITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNITQPLKIILDNISNVL
                     "
     gene            3630..4151
                     /locus_tag="ACGI8A_003041"
     CDS             3630..4151
                     /locus_tag="ACGI8A_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFH0181023.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKIFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIMSGILGIFFYL"
     gene            complement(4280..4516)
                     /locus_tag="ACGI8A_003042"
     CDS             complement(4280..4516)
                     /locus_tag="ACGI8A_003042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181024.1"
                     /translation="MLECFIAKDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSKKFCQEAYDWFVENQ"
     gene            complement(4826..8254)
                     /gene="uvrA"
                     /locus_tag="ACGI8A_003043"
     CDS             complement(4826..8254)
                     /gene="uvrA"
                     /locus_tag="ACGI8A_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFH0181025.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEALNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNHLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQAPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            8573..10807
                     /locus_tag="ACGI8A_003044"
     CDS             8573..10807
                     /locus_tag="ACGI8A_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0181026.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
BASE COUNT         3996 a         1330 c         1660 g         3874 t
ORIGIN      
        1 aattatttga aaatttaata attttataga tatgaaagtt attttcttct aagaaaacag
       61 actaaatgcg tagaatattt ggtaaaactt acctgtataa attgaaatca gaaggtttta
      121 ttattgattt agattagaac aatttgaggg agtgacataa aatgaaacaa caagaagtag
      181 ttaataaact acgcagggga gaattgatgt tgatggaata cttgtggagg aaaaattcta
      241 ttttatctaa aaaagaaata atagaagcaa tgaaagaaaa atataagtgg aggaaaagta
      301 ctacagaaat tctattaaaa agattagtaa aaatgaaaac attaaaaaag aagaggatag
      361 gttttcagtt tagttatgaa gtgctagtaa ctaaaaaaga atatttaaat gcaataaaag
      421 aagatagaaa ggtaactaaa tatgatagtt tttttatacg aatgtttacc actatgcata
      481 aaaaagaaaa gatgacagaa gaacaaataa aaaaatatat agaaaatatg gaaaaaattg
      541 gaaaagaata atttatatat atattaatta tttttaaaat aaatttgtta taatatttaa
      601 atatacataa acatacataa atttacaaaa agggggtgta gcttatggga ttcaagaaat
      661 tgccagattc tgagttaaaa gtaatgaagt ttatttgggg attagatgga aataaggtag
      721 catcaggaga agttgtggaa gcaatgaaac aaatttatga ctggaataaa aatacgacat
      781 taacaatatt gtctaagctt gcaaagaaaa actatttgta ttctcaaaaa acaagcaagt
      841 gtacatatta tactatcgct gtaagagaaa aagattattt aaaagttgaa acaaaaaaat
      901 tctttagttt ttttcataat aattctttaa aaagtttttt cactgcttta aatgatgaag
      961 aaaatttaag tgatgataaa ttagatatgt tagaggaatg ggtgaaaaat tgggaagaag
     1021 atgaataaaa tgaatgtgat actaaaatga tactaatatg ttttatagcg tgttaaaaaa
     1081 tatagaaaat atagaaataa tagaagaaaa ttaatgtaat agataaggtt ttatcagatt
     1141 aaaaagaaaa tcaatagaat gggaataaaa tatagaatgc caaaaatcca cataaataca
     1201 ggatttaagg tctctcttac ttctatgtta tactggtttg atactatcaa tatttgccag
     1261 tatcttatgg attaataagt aaaaaacatc ctgctgaatg ataaattcag cggggtgttt
     1321 ttattttgtt ttagaactcc tttgctctga aactcgaagt atttaacact gatgattttg
     1381 ttatctttta gttccttttg tgtcatgctc tgaaagacaa attgatacaa taaaaaagta
     1441 atttcttaat cattgatgga gatatgtatt tatgttactc ttgatggtaa tttgagtaat
     1501 attgttcata gctttttata ttcaattaat tagaaaacca tttggtataa tcataaataa
     1561 acttgatgat ataatagaaa agatttataa ttattagaag tatataaaag tattttatgt
     1621 ctatgaaatg tatgtctata aaacatatag atgacttttg tatattaatg tatgaatatt
     1681 gatattatag aagaattgag ttgtcaatga tactaaatca aatatttata gaagctggag
     1741 ggattatgag aaataaaata attataggaa ttactttttt acttgtatta ctaatgtctg
     1801 gatgtgcttc aaaacaaacc gcagaaataa acgtattagc aactactgat ttacatggag
     1861 ttatacctta tgagttaact tcttatgtaa aagaagaaag aaaaaaagat aaaaatctaa
     1921 ctttagtaga tgcaggagat ttttttgata gtggagaagt gtttggttct agcatggata
     1981 gttattttag tgctagatat aaagatggtg ataataacac tgaaagttat attgagacgc
     2041 ctatttcaaa agatatgaaa gaggtaggtt atgatgttgc agttcttggg aatcatgaat
     2101 ttatatccaa caataaattt tatctagata atatgatatc tgattttgag aagcaaaaaa
     2161 taaatatttt atcggcaaat acgtataaaa aaaatggaga cagttataca aaaccataca
     2221 taataaaaaa tattaataca cctgaaggaa acgtaaaact aggaatttta gggcttacta
     2281 taaaagaagt tggtgagagg aaacagtgga aagatggaaa acttgttgac tctaagtctt
     2341 tagaactaaa agaccaagag ggctacaatg gtgaattgta tatgaatgat ttagtagaag
     2401 atgctaaaaa gtgggttagt ataatggaaa aagagaagcc tgacattata gttgctgtgg
     2461 cacatacagg tgagaaacct aagaaaccta aaaacccagg aaatagaata caagacttag
     2521 ctcaacaagt tgatggaatt gatgctatag ttgcaggtca taatcatgtt caaattgagc
     2581 aacatgatta taaaaataaa tcaggagaaa atgttatagt tactgagcca ggaaagcatg
     2641 gggaatgtat ttctaagatt aattttaaat tagaaaaaaa caatgataaa tggaatatag
     2701 ttgacaaatc tagtaaatta attaaatttg aaaaaaatca agaaagtgat aaatttggag
     2761 aatttatttc ttcaattaat aaagtaagta aagataagaa agaaataaga ttatcagaag
     2821 taaagactcc tgaatgggat aaaatatatg tttttaaatc tggaacatct gcagatgata
     2881 tatataaaac aataggatat aaatggagaa atatatcatc tccaaagagt gatggcatgg
     2941 tgcagattgt tgtaatgaat aataataagc ctgttagcta tatatatggt gaagctgaag
     3001 ttatgccaat taatataaaa tgtgatgaat cagagtataa agataatgtt gtaactattt
     3061 atcctaataa aaatgataaa tttaaaattg aaaaaggtaa acaagaatat agtattaatt
     3121 taaaacatat acaatagaaa cttttatctt atttaagttt agattgtact taaaataaag
     3181 tgtgtattat tttaaaaatt gatgataatt aaaatcacaa cattaaggaa tagtacacac
     3241 tttttatgct aaaaaataga atgttaaaaa attaaacctt taaattaaag tacattgctt
     3301 atattatcca atattatctt caaaggttgt gtaatatttt tatccttatg taatattgct
     3361 aacattttat tttccatatc tatcccatct aattcaatca atcgcaattt tttatgtaag
     3421 agtgaatcta ataataaatt ttctggcaaa atggttatac ctaaccctgc ttgtgcagct
     3481 ttaataagtg caaaagaatt aacactttcc caaacaggat aagctttttg gtttgaaaga
     3541 gataatacac tgtccaaagt atcgcgaatg gcacttcctt gctcacgtaa caataacgga
     3601 aacgtgcata actgtcgtag agataaggct tgtttagtat tggtggtggt tatgcaatca
     3661 tacctttaat ccaagagaaa gtagtaaatc aaactggatg gataagtgag aaaatattta
     3721 cagatattat aactatttca caaatgacac cagggccact ggctgttaat acttctactt
     3781 ttgttggact tcaaattgga gggttttggg gagcagttgc agccacttta ggttgtgtat
     3841 tatgtggtgt agtaatatct ctatttttat ataatttttt tcaaaagcat cagaactcta
     3901 cttatatatt tgaaatatta aatggtctaa aatcagcttc attaggttta attatctcag
     3961 ctgcggctac aattataatt cttgcattat ttgggacgaa taaagtgtac ttcaattcta
     4021 tatttgagat gctaaactgg actgcactaa taatcttttt tgttacacta ctgatagtac
     4081 gtaagtggaa aattaatcct attgtgatta taataatgag tggtatactg ggaatatttt
     4141 tttatttata agtttttgct aagtaaagga tatctatttt tttatatctt aaatccaaat
     4201 agccaataaa gtaaggctat ttggattttt aataaattct gaaaagataa tcagaaacct
     4261 atataacatt taaattatat cactggtttt ctacaaacca atcataagct tcttggcaaa
     4321 atttcttact atctatatag taactattat gaacattatt aatttcaaca agtttctttt
     4381 tacattttag tgaattataa tagctatcaa aatattgggt tttaaaatat ctatcatgaa
     4441 gtgttttaaa gacaagagta ggtatgttga ctccatcttt tatcttacta tcttttgcaa
     4501 taaagcattc taacattgta ttaattatct ttgtactatt tttccaaata tgaggatttt
     4561 tcttttcaaa tattttataa aattcattat catagacttc attataacct ttatatttag
     4621 gtggttttac tgttatgtga aaattaggaa gaaacttaaa gaaaaaatat aataattttt
     4681 atgttctgtt ttatatatta ttcatatttt atcacagata atatactaaa gttaattttt
     4741 ttattgtaca actattataa taattcttct ataatataag acattctata attttatatt
     4801 taagttcatg tgtatttaat tgtttttata taaaatgtcc agtaatacta tttggattta
     4861 tttttatttc atcaggagtt ccagaagcaa caatttcccc tccatttatt ccactgtctg
     4921 gacctatatc gataatccaa tcagaagcac gtataacctc tgtattgtgt tcaataatta
     4981 aaagtgaatg ctgttcattt acaagttttc taaaagtaat taacaaattt tctatatctt
     5041 taaaatgaag tccagtagta ggctcatcca atatgtacag cattcgtttt ttagtttttg
     5101 tccctaattc ttttgctaat tttatccttt gagattctcc tccacttaat cctaatgtat
     5161 tttgtccaag ctttagatat ccaagtccca cttcatctaa taaggagagt tttctatata
     5221 tatcagagca atcgctaaaa aatgtttttg cttctgctac ttccatgtca agtatatctg
     5281 caattgtctt tcccttgtat tgtatagaca gtatttcttt ttgatatctc ttaccttgac
     5341 attcactaca agttacccat tggtctgcca tatattgcag aataattttt gttttaccta
     5401 atccttgaca gtttgggcat tgcccctttt tgctattaaa gctaaaatag ctttcatcca
     5461 ataatttttg ttttgcttgt ggagtttcag caaatagaga acgaatttca tcaaaaatat
     5521 tgatatatgt agcaggtgta gaacggctag attttccaat aggtgcttgg tccattaaga
     5581 taaaatcatc aatagcatca aatccagtaa attcagtata atttttatct gggatagatt
     5641 tttgtttcaa tttttcttct agagcaggaa gtaaactgtg aaatacaaga gagctcttac
     5701 cagaaccact aacacctgta atagagcaca tacagtttaa gggaatttct acatcaatat
     5761 tttttaagtt atttgcatgg catcctttta agcttaacca tttacttggt ataattttat
     5821 tttgtgaact tattttattg tatgtactta aatatttacc tgttatagag tttggatttt
     5881 ttataatttc ttgggttgta ccttctgcga taatgaaccc cccttttgta ccagcacttg
     5941 ggcctacatc aataatataa tcagcagaca gtattgtatc tttatcgtgt tccacaacta
     6001 taacagtatt acccttatct cttagctctt ctatcatttt gatgattaaa ttatggtctc
     6061 ttgggtgtaa accaatagat ggttcatcta aaatgtaggt taagcctact aattcacttc
     6121 ctagctgact tgataatcgt actctctgca gttctcctcc agataaagtt ggagctgttc
     6181 tatctgttga tatatagcta agacctacct ttaatagata actaactcga atttttaatt
     6241 ctgttaagat atcatttacc aaagagagct catttttttg aaggtgattt ggtaattcac
     6301 acaaccaatt ccataactgt tctatcgtca tttttgttag ttcagggaag cgataacctt
     6361 tgattgtagt gaatctagcc tcaatgcaca gcaattcgcc accacatgta ttgcaaggaa
     6421 ttttgttcat atattgatgc aaggtattat tttcagaact agattctcta aaaagtcttt
     6481 gaatgtggtt gattgctcct gaagcaggtc ttctaatctt tgattctcgt ccctttgatt
     6541 caaagctaaa ttcatatatc ttatcgtctg taccatataa gattgcatct ataaaatctc
     6601 ttggtaattc attccaaggt atatctaggt ctatattttt atcttttgct attgcatata
     6661 attcacctac catccaatta ccatttggct ttttccccct caattttcca aaatagtaag
     6721 tagctccatc taatattgaa atagttgggt cttttacaat taattgagga ttaacttgat
     6781 aggtaaaacc taatccatta caatcgtgac aagctccagt atgggagtta gcattgaagg
     6841 ttgaagtatt tatcttagaa aaaatagttt gacaatgtgg acaataattt tttgtattat
     6901 tctttttttc taatggaatt ttgcaatatg gacagtaact ttttccgatt gatacaaata
     6961 acattcttaa ataatcccaa atgcttgtaa gagtaccaac agtagagcga ggattttggc
     7021 ttcctttttt ctgttcgatg gctatagtag gggttaatcc ctgaagtaaa tcaaattccg
     7081 gtttttgaag ctgttctgaa agttttgctc cgttacttag attttcaaaa taacgtcttt
     7141 ttccttcttc ataaatcgta tcgaaagcta aagaagattt tccactacca cttacacctg
     7201 taataacagt tatttgattt tttggaattg taacttcaac atcctttaaa ttgttttgcc
     7261 ttcctccttt taaatagata gagcttttat tatgacaaag tgaattttta attgatttat
     7321 ttattgcttt tgtaaatttg aatttctttt ctagttcaag aggattgcct aattctttta
     7381 tttccgaaat acctaatatt tttaactgag aaaagattga tttcaaataa tcttcaggca
     7441 atttttctat taacaaatta agggcttcta tttttatatt tccgcttgat aatatctctg
     7501 ccatttcatc taataaattt ttttctgctc ttgttaattg attgtatttt ttggaactta
     7561 attctgtcca taaatcccat ggaagcaatt ctaaaccaat catattatta aagtcatgta
     7621 gaagattacc ctttacagaa tgccaacctt tccaattttt attatatcca aagtcatctg
     7681 gatgagcatg tccttgccga catcttatcc aggcttctga accagttaaa aaaccatctg
     7741 tttttccgac atcatgagtg tcaaaattaa tttgaaaata atttttttgt atgtcatcta
     7801 tttgtgcatc tattcttatc catctctttg ttagagtatc atagtattct acaagccagt
     7861 ggtcttcata agtaagctca ctttcaaaat aattagcaaa acctgctctc attctagcct
     7921 caaaacctcg atatcttagg agcgatacaa gaagtaaaga ataatctctg cacattccaa
     7981 caagcctttg ttttgggttt cttggaatag ttagtgggtt agaatttatt tcaaatattc
     8041 tttttaacat ttgaggaatt gtacgaagca attcctcatc agattgttgc ttattaaagg
     8101 atataccata taattttcct tggtctccat gaattactag gctttgaacc acttttaata
     8161 actcaattgg agtctttggt aaatcttgat atttattttc aaatttttta ggatttgtga
     8221 tgatactttg atttttatag ttatttattt tcatttatat ttcacctctc taaatggatg
     8281 atataccctg acatggtgtc atatgcaagg acaatttaga tttttggcca aaatttaaaa
     8341 aataaataaa aaatgataca gtgataaatt gtaatacaaa tatagtttca aattgcatat
     8401 aatataatta tttaataaaa ccattatcta ttgaatgtta atgaaatgtt aagatggcat
     8461 tcttgaatat taagaaatag aatactataa tatagtgtga aggattttta gtaaaaaaat
     8521 ttgtaagtgt taatatacat taaaaaatag gtttataatt tggaggaaaa aaatgaaatt
     8581 atcaaggtat aagaaaaata gtttagttct gtctataatt ttatgttcac ttattatttc
     8641 aatccttgta attactgttt ttaataccgt aaaactacac aatatatcta ataaacaatc
     8701 taaaaactat ttaaatgatg tatctacaca gattgtaatg aatgttgatt ctaaaatcaa
     8761 atttatttta tccgacttaa gaataatggc agattttatt aagcaatatg aaggtgatag
     8821 tcgatatgag tatttatcta aaagaaagac tagttataag tattatgatg ttggaataat
     8881 agatttagaa ggtaaagctg agtttttaag tggaaaaaag tttgacttaa agaatactgt
     8941 ttcctataaa aaaggaattg aaggtaaaga atactcagaa gtaatagagt caatgggttt
     9001 tgttctttat agtgtaccta tatttaatga taatcatgaa gtatctagta ttttaatagg
     9061 tgtatccaat aaagaaagta tgaatgagat tcttaatata gataacttta atggaaaagg
     9121 aactattgaa attattaata gtaagggaaa acctcttttc ataggaaaaa atactgaatt
     9181 aataaaggac cttagccata aatataagag ttctggagat gaaccttggg cacaaaagat
     9241 gcttgaggat tttaaaaatg ataaatcagg gaatattaca ataacctcat caaaaggtgt
     9301 acaatgctta ttaacttatc atccaattac aagaggttta aatgattggc attttcttct
     9361 catagtacct gaagatgctg tacttggtga acttaataaa ttaaactctt ttacaataac
     9421 aatgacattt ataattacat gtattattgg aattataaca ttaattttat atattataag
     9481 aagaaaatat gtaaatcaaa tagaaaatat tgcatattta gatagtataa caaatggtat
     9541 aaattcaact aagtttagta tgctagtcaa acctttaatt tcagaatcac ctgatagtac
     9601 ttatatgatg atagcaatga atataaaaga ttttaagcta ataaatgatt gtttcggtag
     9661 tgaaaaggga aatatgacac taaaacatct ttacaatatt ttgtataaaa acattgatga
     9721 aaatgaagaa tttgtgtgcc gtcatgatgc agaccttttc tatttgctta ttaagaatag
     9781 accagtgtta gaagccctgg agttattata taaaatagaa gctgacacaa attattttaa
     9841 taaaaatagg gaaaatccat actttttaag actatcagta ggtatttaca caattgaaaa
     9901 tcataatgaa gatttaatta caatacaaga ccatgcaaat acagcaagaa aaagctttaa
     9961 taaaactcat caaagtgatt tttcttttta tagtgatata gaaagaaaac gattaatatc
    10021 tgaaaaggaa atttcaaatt taatggaaaa agcccttgaa aataaagaat tttttatgtg
    10081 cttacaacct aaaattgata taaaaagtgg aaaaattagt ggagcagagt ctcttgtgcg
    10141 ttggaaaaat tcagaaaagg gcataattta tccaagtgat tttattcctc ttttcgaaaa
    10201 aagtggattt atatgtaagt tagatttata tatactagaa gaaacatgca aattgatttc
    10261 taagtggata aaagaaggta aagaagtgtt gagaatttct gttaatgtat cacgtcaaca
    10321 tctgaatgat aaattgtttt tagaaaaata taagcatata tgcaataaat acaatgtacc
    10381 aacatgtctg atagatttag aacttaccga atctattttc ttagaaaatc cagaagctat
    10441 agatataata gaggatattc attctaatgg atttaaatgt tctatagacg attttggatt
    10501 tgggtactca tcattaggaa tattaaagga ttttaaagtt gacataatta agttagaccg
    10561 ttcattcttt gttagcaaaa ataatataga ccgtggtaag gtagttatta agtctattat
    10621 tgaacttagc aaaaggctag gtatgaaagt aactgctgaa ggaattgaag aattagaaca
    10681 agttgagttt ctaaaaaatg tgggatgtga ttatattcaa ggttatgttt tttcaaaacc
    10741 attattaatt tctgattttg aaaattttgc atatgaaaat aataaaatta aaaatatcca
    10801 ttattaaatg tacaagataa aaaatttagg caagttatat aattaaaaat gtaacttgtc
//
LOCUS       DAYATN010000131        10722 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.131, whole genome shotgun sequence.
ACCESSION   DAYATN010000131 DAYATN010000000
VERSION     DAYATN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.131"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            262..720
                     /locus_tag="ACGI8A_003045"
     CDS             262..720
                     /locus_tag="ACGI8A_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0181027.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            781..1056
                     /locus_tag="ACGI8A_003046"
     CDS             781..1056
                     /locus_tag="ACGI8A_003046"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0181028.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1115..2389
                     /locus_tag="ACGI8A_003047"
     CDS             1115..2389
                     /locus_tag="ACGI8A_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFH0181029.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2519..3571
                     /locus_tag="ACGI8A_003048"
     CDS             2519..3571
                     /locus_tag="ACGI8A_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFH0181030.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3571..4602
                     /locus_tag="ACGI8A_003049"
     CDS             3571..4602
                     /locus_tag="ACGI8A_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFH0181031.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4694..5506
                     /locus_tag="ACGI8A_003050"
     CDS             4694..5506
                     /locus_tag="ACGI8A_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFH0181032.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5509..6429
                     /locus_tag="ACGI8A_003051"
     CDS             5509..6429
                     /locus_tag="ACGI8A_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HFH0181033.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6604..7050
                     /gene="rpiB"
                     /locus_tag="ACGI8A_003052"
     CDS             6604..7050
                     /gene="rpiB"
                     /locus_tag="ACGI8A_003052"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HFH0181034.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7080..7745
                     /locus_tag="ACGI8A_003053"
     CDS             7080..7745
                     /locus_tag="ACGI8A_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFH0181035.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7855..9357
                     /locus_tag="ACGI8A_003054"
     CDS             7855..9357
                     /locus_tag="ACGI8A_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFH0181036.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9669..>10722)
                     /locus_tag="ACGI8A_003055"
     CDS             complement(9669..>10722)
                     /locus_tag="ACGI8A_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFH0181037.1"
                     /translation="SNLLNMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFFMYIYYA
                     EAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVIGDDEI
                     STLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDF
                     DRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFE
                     DKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEE
                     NIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHMEII
                     IDADLFKVMLTFRRVN"
BASE COUNT         4271 a         1217 c         1972 g         3262 t
ORIGIN      
        1 aaaaaatcat ttctctaaca aaaaaaacca caaaaaatat aaaaaagtat ggctagttaa
       61 gtgattaaca agtaaaaatt aaaataaaaa tcaaaaaaaa tttatcaatg aaagtcagct
      121 aaaatcctgg aagagagcat tatttttggg aaaacatttt cttttgtttc acaaaaaaag
      181 ccactttttg gcacacaaca tgctttatat aaagtcaaga aaaactaagg tataagacaa
      241 aggcaattag gaggtgtaaa tatggagttt ataaattttg taagtgataa agttatagac
      301 cttaaaatgg attgtaaaac gaaagaagat ttctttaaag aaatacataa taaagttttt
      361 gatttaggct ttgtaaaaga agaatttgga gaaaaaatat tagcaagaga aaatgtattt
      421 ccaacaggtt taaatttagg tgattatgga gtagcaatac cacatacaga tgcggaatat
      481 attaaagagc aatttatatc agtctgtact tttaatgaac cagtagtatt tagttctatg
      541 gaagaccaag atgaaaaagt acctgtaaat ttggctttcg tattaggact taatcaacca
      601 cacagtcaat tatctgtatt aacagaatta atgggaatta tgcaaaataa agagttagtt
      661 gataagttga tgagttctac tgataaagaa gaagttttaa agacactaaa gtctttataa
      721 aaataaaaaa gtactaaaaa taaaaaatat aaaaataaaa attattagga ggaaataaaa
      781 atgaaaaaaa tattagtagc atgtggagca ggaatagcaa catcaacagt agtttgtgac
      841 aaggtagaga gattagttaa ggaaaataat atacaagcag aagttataca atgcaaaata
      901 gctgaatgtg caacaaagca agaaggagca gatttaatag tatcaacaac tatactacca
      961 acaacttata atataccagc aataaaagca acagcatata taactggagt aaatacagca
     1021 gcattagata aaaaaatatt agatgcttta aaataatcct atcatagata cattgaaata
     1081 atattttata aaacagaaaa aggaggattt ataaatggat aaattattag cagtagtaca
     1141 atacatttta aatatgggac cgacagtaat actaccaata acaattgtag tactaggaat
     1201 gatatttaga attaagttta caaaagcttt aaagtcaggt cttacaattg gtataggatt
     1261 tgtgggtata aacttagttg taaacctatt aacatcaaca ctagggccag cggctcaagg
     1321 aatggttgaa agattcggac ttaatttaac agtaatagat gttggttggc caacaacagc
     1381 atcagcaaca tgggcttcac cagttgcacc aatattaata ccagtttgtt tattagtaaa
     1441 cttactatta ttatacttca ataaaacaaa gatattaaac gttgatatat ggaattattg
     1501 gcactttata gtagcaggtg cagttggata tatagtaaca ggaagttatg tttgggctat
     1561 attctgtgca atactaatgg agttattagt actgtttatt gcagaaaaga cagcaaaaaa
     1621 tgttcaggag ttttatgatt tagaaggtat agcattacca actggttcaa cagcttcatt
     1681 tgctccactt ggatatttag taggtaaatt tgttgaaaaa ataccaggaa taaataaaat
     1741 acaagcagac ccagactcta tacaaaaaag atttggtata tttggagaac caatgatgat
     1801 ggggattata ctaggtataa tacttggagc acttgcggga tatgatgtag caggaatatt
     1861 caatgtaggt atatcaatgg gtgcagtaat gttacttatg cctagaatgg ttagaatatt
     1921 aatggaaggt ttaattccaa tttcagaatc aataagagat ttcttacaag caaaatatgg
     1981 agacagagaa ctttatatag gtctggatgc agcagtagca actggacatc cagcagttat
     2041 atcaacagct cttatacttg ttcctataac tttgttctta gcagttatac taccaggaaa
     2101 caaagtttta ccatttggag acttagcgac aataccattt tatatggcat ttatagtgtg
     2161 ttttagaaaa ggtaatatag ttcaatcagt tataacagga acaatagtaa tagctatatc
     2221 actttatatg gctacaaact ttgcagatgt tcatacacaa ttattacaac aagcaaatat
     2281 gatgccagaa ggaacaacaa tggttacaag tatagataca ggtggaaact tgctaaaatg
     2341 gggaatattg aaattatctc aattagtttc ttcatttgga atattctagt atgtagttaa
     2401 tagagattac tcttttttag taaagtatgc gaaaatttaa catttagcag ctactaaaaa
     2461 agagtaatat tttttataaa tttttaaaaa tacaaaatac aagaggagga gatttatcgt
     2521 gaaagcagca gtattacatg gcacaaatga tatgagattt gaagatatag aaataaaacc
     2581 atgtgaaagt gatgaagtaa aaataaaagt tatggcagca ggaatatgtg ggtcagatcc
     2641 tccaagagta ttaaaacatt ggaaatatcc agttccagct ataccaggac atgagttttc
     2701 tggagtaatt gctgaagttg gtaaggatgt aaaaaatgta aaagttggag atagagtagt
     2761 tgcaattcct tttatacctt gtaatgaatg tgaatattgt aaaagaggat tattttcatt
     2821 gtgtgatgac catggaatgt taggagctaa aagttttgga gcttttgcag agtatgtaaa
     2881 tataaaagct acaaatgtac ttccaatagg agatatgaac tttgaagatg ctgcaatgat
     2941 agagccacta gcagttgcta tgcatggagt acttaatata ggtgttcaag ttggagacac
     3001 tgtagctgta atgggtagtg gaacaatggg gcagttggta atacaaggac ttaaaatagc
     3061 aggggcagga acaattatag cagttgatat atctgataat aaattaagag aatcaaagga
     3121 attaggggct gatataataa ttaatgctaa agatattaac ccagtagaaa aaataaaaga
     3181 acttactggt ggaaaaggtg ttgacatagc tcttgaatgt gcaggttcaa aaataactca
     3241 agaacaatgt ttacttataa ctaaaaagaa aagtaagata ggatttttag ggatagcata
     3301 ttcagatata actttatcag aagaagcatt tgaaaatata tttagaaaag aactagaatt
     3361 aaaaggtttt tggaactctt attctgcacc atttccagga caagaatgga ctaagggaat
     3421 caatctagtt aatgaaggaa agataaaatt aaaagaaatg gtatctcata gattctcact
     3481 tgaagatact tacaaagcat ttgaaatgat aagagataga aaagaagaat ttaacaaaat
     3541 attaatttta ccacaagggg ttgaaaaata atgaaagcag tagttaaaac taaaccaggc
     3601 tatgataatg tagaagtttt agaagtagaa gaaccaaaag ctacaggaga caaggttaaa
     3661 ataaaagtag aatatagtgg tatatgtggt tcagatatac attcatttaa aggtgaatat
     3721 gcaaatataa aagccccagt aacattaggt catgaatttt ctggaattgt tgtagaagta
     3781 ggagaagatg ttaaaaatat aaaagtagga gatagagtta catcagagac tacttttgca
     3841 acttgtgaga aatgtatata ttgtgaaact aaagattata acttatgccc aactagaaaa
     3901 ggtattggta ctcaagtaaa tggaagtttt gctgagtatg tattatcaag agaagaaagc
     3961 atacatgtac ttcctgaaaa tgtatcttta ttatcagcag cccttacaga gccactagct
     4021 tgttgtgttc atgcagctct tgaaaagact actatagaaa gttctgatac agttttaata
     4081 ataggaccag gaccaattgg tttattatta gctcaagttg ttaaatctca aggtgctaca
     4141 gttataatgt ctggtgttac aaaagataag gaaagattag aaatagctaa aaatcttgga
     4201 gtagatagaa ctgtaaatgt aatggaagaa agtttggcag atgtagtgaa tgaaatgact
     4261 gatggaaatg gagtaaacaa aggatttgat tgttctgggt ttatgccagc agttaacgaa
     4321 gccttaagac tgcttagaaa aaaaggtact ttcgtacaag ttggaatctt tgctaaaaaa
     4381 ttaaatgaaa tggaccaaga agcaataatc caaagagagt tacaatatat aggaagcagg
     4441 tctcaaaagc caagttcttg gattacagca ttagatttaa tggcaaatgg gaaagtaaat
     4501 acagaagctt tagttactag aactgttggt ttagacgaat ttaaagaagg tattacagct
     4561 ttaatgaacg gggaagaaat aaaagtagca gtaaaatctt aaaaaaacat ttgtattatt
     4621 ttaaaaaaaa ttgtaaaatc tactgtagga taaaaaaatg taaggtctta aaaaataaca
     4681 ggaggaagaa agtatgagca ctcttagaga tcattctaat aatattagaa aaaacataat
     4741 aaaaatggtt gcagaagcta aaagcggaca tccaggtggt tcattatcaa tagctgatat
     4801 gttgacagta ttatattttg ataagatgaa cgtatgtgca gaaaatccaa aaatggctga
     4861 tagagataga tttgttctat caaaaggtca tgcagcacca gctttatatg ctacattagc
     4921 agaaaaagga tttttcccag aagaagaatt aataacacta agaaaatttg gttctaagtt
     4981 acaaggtcac ccagacatga aaaaagtagc aggaatcgat atgtctactg gttctcttgg
     5041 tcaagggcta tctgcagcaa atggtatggc acttgcagga aaattagata acaaagacta
     5101 tacagtatat actatattag gtgatggaga aatccaagaa ggtcaaatat gggaagctgc
     5161 aatgacagca tcacattaca aattagataa tttaattgca tttgttgact taaatggatt
     5221 acaaatagat ggttctaatg aagaagtaat gaatgtaagt ccagtagatg acaaattcaa
     5281 atcttttgga tggaatgtaa tagtaataaa tggacattct tttgaagaaa taggaaatgc
     5341 agttgatgaa gctaaaaaag ttacaggaaa accaactgta ataatagcta aaacagtaaa
     5401 aggtaaaggt gtttcattta tggaaaataa tgctgcatgg catggaacag ctccaaatgc
     5461 agaacaagca gagcaagcat tgaaagattt agaaggaggt ctataggaat gggaatagca
     5521 actagagaag cttacggaca agtgttaaaa gaacttgcag aaaataaaga tatagtagta
     5581 ttagatgcag atttaggaaa agctacaaaa agtatatcat ttaaagaagt tgccccagat
     5641 agattttttg acatggggat agcagaaggt gacatgattg gtacagcagc aggtcttgct
     5701 acttgtggaa aaataccttt tgcgagtact tttgcaatat ttgcagcagg aagaggctat
     5761 gagcaaataa gaaactctgt agcatatcca aatttaaatg taaaaatagc agctactcat
     5821 gcaggtgtaa cagtaggtga agatggtggt agccatcaag ctatagagga tatatctcta
     5881 atgagaggta taccaaatat ggttgtatta aatccagcag atgctttaga agcaagacaa
     5941 gctattctta catctattga ttataatgga cctgtttata taagattagg tagagctgca
     6001 actcctgatg taaacagtga aaattataaa tttgaaatag gaaaaggaac tgttttaaga
     6061 gaaggttctg acataacagt aatagctaca ggaataatgg tagcaaaagc cttagaagca
     6121 gcagaagaac ttgctaaaga aggtataaat gtagaagttg ttaatatatc tacaataaaa
     6181 ccattagatg aagcattaat aaaagaaact gctaagaaaa ctggaaaagt agttacagca
     6241 gaggagcaca gtataatagg tggtttaggt tcagctgtat gtgaagcttt agcagaaact
     6301 aaagatgtag tagttagaag aattggtgtt aaagatgtat ttggtcaatc aggtacacca
     6361 gctgatttat taaaacacta tggtttaact acagaagata tagtaaaaaa tataaaagag
     6421 ttgttataga tataaatata tctaaataac aaattgattt gacataagtc aattaaagct
     6481 gtcttaaaaa tagaaagaaa tttctacaat gggacagctt tatttttaca atactttatt
     6541 tttatgataa ttataaaata gtctgaaagt aatcaaaaaa attataaaaa gaggtgctat
     6601 ttaatgaaaa tatctattgg atgtgaccat ggtggttatg aattaaagga atatataaag
     6661 gaaaatttaa taaataaagg tatagaagtt gaagatgtgg gtacaaattc aaaagaaagt
     6721 gtagattttc caatatatgc aaaaaaggta agtcaaaatg ttcaaagtaa aaaatctgat
     6781 ttaggcatac tttgttgtgg aacaggaata ggtatgtcta ttgcagcaaa taagtttaaa
     6841 ggaatcagag cagctgttgt ttctgactgt ttttcagcta aggcaactag agaacacaat
     6901 aatacaaatg tactttgttt aggagaaaga gttattggga aaggattagc aatgagaatt
     6961 gttgaagaat ggttaaattc aagttataat ggagaaagac atgcaagaag attacaaatg
     7021 attgatgata tagaaaaaga aaatttataa atatataatt tataacaagg gggagcgtta
     7081 tgtctattat ttgtgcttca ataatgtgtg cagaccaaat gaagttaaaa gaggaattag
     7141 aagctcttga agaagcaggt gtaaaattac ttcattgtga tgttatggat ggaatatttg
     7201 ttaaaaactt ggctatggga ccagagttat taaaatcaat aagtgaaaat acaacaatac
     7261 ctttagacat acacttagcc acagaaactc cagataaata tattgatatg atgtcttata
     7321 taaagccaaa atatatttct tttcatgtag aatcaagtac aaatgtaaaa gctgatatac
     7381 aaaagttaag aaattatggt atagggccag tgttagcaat aagccctcaa acaagtgtgg
     7441 ataaaataga agaatatata agtttagttg agggtatctt agtaatgaca gtaaatccag
     7501 ggtttgcagg tcaaaagttt aatctttctg tattggataa gttggataaa cttacagaga
     7561 tactaaaaga ttatgataat cctccattta tagaggttga tggaaatata aataaggata
     7621 ctattaaact tatgaatggt aaaaaagtag atatatatgt tgttggtact tcagctttat
     7681 ttaatgataa gcctccaatt tcatataaag ataaaataga agaactaaaa gaaagtataa
     7741 agtaaccatg taataaaatt ataatatgtt tctgtttaat agtaaaaatt taggtggaaa
     7801 caagaaattt tataatattt aataaaaatt ttggttgagt aaggagaatt ttttatggga
     7861 gacctatata aactacaaaa tggtactgat attagaggaa tagcgtatga aaatgattct
     7921 aaagaagtta acctaactgt agaagaagta aaaaaaatag ctaaagcgtt tcatatttgg
     7981 ttaaaagaaa agactaagaa agataaagta actgttgcca taggaactga ttctaggata
     8041 acaggaagcc aatttagaag tactgttatt gaaactttaa ctaatgacga ttgtaatgta
     8101 attgattgtg agattgccac aactccagct atgtttatga caactattat ggatggatac
     8161 aattctgatg gggcgattat gattacagca agtcacttac cttattacta taatggttta
     8221 aagtttttca cagaaagtgg aggtttagaa aaaactgata taaaagagat gttggatata
     8281 gctgttaaaa atgatagtgt agactatgag gaagtaaata aaaaaggtga agttatagta
     8341 aaaaatttaa tagaagatta ttctaattta ttgatagata aaattagaaa aggtgtaaat
     8401 tcaagtaaaa attatgaaaa gcctttttct gggcttaaga tattggttga tgcaggaaat
     8461 ggggctggtg gattctttgc tgaaaaagtt ttacacatac tgggagcaga tacgacaggt
     8521 agtcagtttt tgaacccaga tggtatgttc ccaaatcata tacctaatcc cgaaaacaaa
     8581 gaagcaatgg aaagcatatg taaagctgtg ttagataata agagcgacct aggaataatc
     8641 tttgatacag atgtagacag agctgctatt gtaggtaaaa atggcaaacc aataaataaa
     8701 aatgctctta tagctgttat atcatcaata gtattagaag aacatccaaa aacagctata
     8761 gtaacagatt cgattacttc tgaaggactt gctaaattta taaatgaatt acaaggaaga
     8821 catcatagat ttaaaagagg atataaaaat gtaataaatg aagctataag attgaatagt
     8881 gaaggtgaag agtgccattt agctatagaa acgtctggac atgctgcttt aaaagaaaat
     8941 tatttcttag atgatggggc atatttaata gctaaaattt taataaaagt agcaaagcta
     9001 tccttggaag gaaagactat agaagaattg atagaaaacc ttgaagaagc tcaagaagag
     9061 aaagaagtta gaataggcat taataaagaa gattttagac catatgcaga ggatatatta
     9121 aaagatttag attctcatgt caagagtatt gatggatggt ctgtagcacc aaataatttt
     9181 gaaggcataa gagttaattg tgatgaatca aatggagatg gatggttttt acttagaata
     9241 tcactacatg aaccattatt agctttaaat attgaatcaa ataaaattgg cggagcagaa
     9301 caaatttatt ctaagttaaa acttttctta gaaaaatatg atttaaaagg tctttagggt
     9361 aaatagaaag aatatattta tattgaagaa taaatgtaaa tttgtaccta agattacaaa
     9421 tattacaaaa ttgtaatctt aggtaacaga gatgtaactg attatggaaa cactatgtga
     9481 tattatagat acagagagat gaaatatgtg atgaattata gagaaatata atttaaaatg
     9541 tattaatatc acaccaaaaa ggtataatca aaaaatatgt aaatataaac cccaacattt
     9601 agaagaagac ctctaaggag gtttttttct tgtttaaata acttaaaata tatttttata
     9661 tcaatatatc aattaactct tctaaaagta agcattacct taaataaatc agcatctata
     9721 attatttcca tgtgtgcatt ttctaattca attatatttt gagcaattga taaaccaaga
     9781 ccagagccat ctgtttttct agatatatca ccccttctaa atctttctat taactcttct
     9841 ggctttagat ttagtttatc atttgaaata tttttaaacg ttaatattat attttcttcc
     9901 tttataaaca tatctatgta gaccctagtg ttaggcatag agtattttag tatattgcaa
     9961 attagattct caaaaactct ccaagttttt ttaccatcta tcatggtaaa aacagcatat
    10021 tcaggtgttt ctattataaa atctaaactg ctattttgaa ttttgtcttc aaactctaca
    10081 atactttgaa ttaaaagttc tcttatatcg gtttttgtta tattaaattc tacatctcca
    10141 ctacttagct tagatgcttc aaatatgtca tctattaata attttaatct ttgtgatttt
    10201 ttatctaaaa tatttatata gtcatttata gtctctctgt caaagtcatt attttttaac
    10261 atatcaacat agcttaatat agatgtaagt ggagttttta agtcatgaga tacgtttgtg
    10321 ataagctctg ccttcatcct ttcatttttt atattagcat ctacagcagt ttcaagacca
    10381 cctgttatat tatttatact tttggctaaa gtagatattt catcatctcc tataacttct
    10441 aatttatact ttaaattgcc attttctata ttttttatat tttttgatat gttatttatg
    10501 taattaaact taacagataa aaagttgtat atataaaaat gaaaaactgt gtttacaagt
    10561 gtaaataaaa ttactatagc tattgcctca gcataatata tatacataaa gaatatcaaa
    10621 acaagatatt ggataatagc ttgtacaact atagatatta aaaatactat agctaatttt
    10681 ttatttatag tttttttatt atacatatta agcaagtttg aa
//
LOCUS       DAYATN010000132        10445 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.132, whole genome shotgun sequence.
ACCESSION   DAYATN010000132 DAYATN010000000
VERSION     DAYATN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.132"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            169..3066
                     /locus_tag="ACGI8A_003056"
     CDS             169..3066
                     /locus_tag="ACGI8A_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0181038.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDEESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKVKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            3387..3599
                     /locus_tag="ACGI8A_003057"
     CDS             3387..3599
                     /locus_tag="ACGI8A_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFH0181039.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            3612..3833
                     /locus_tag="ACGI8A_003058"
     CDS             3612..3833
                     /locus_tag="ACGI8A_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFH0181040.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            3933..6089
                     /gene="feoB"
                     /locus_tag="ACGI8A_003059"
     CDS             3933..6089
                     /gene="feoB"
                     /locus_tag="ACGI8A_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFH0181041.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            6115..6267
                     /locus_tag="ACGI8A_003060"
     CDS             6115..6267
                     /locus_tag="ACGI8A_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFH0181042.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            6447..7157
                     /locus_tag="ACGI8A_003061"
     CDS             6447..7157
                     /locus_tag="ACGI8A_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HFH0181043.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            7242..8012
                     /locus_tag="ACGI8A_003062"
     CDS             7242..8012
                     /locus_tag="ACGI8A_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181044.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            8005..8736
                     /locus_tag="ACGI8A_003063"
     CDS             8005..8736
                     /locus_tag="ACGI8A_003063"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181045.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            8770..9729
                     /locus_tag="ACGI8A_003064"
     CDS             8770..9729
                     /locus_tag="ACGI8A_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0181046.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(9827..10423)
                     /locus_tag="ACGI8A_003065"
     CDS             complement(9827..10423)
                     /locus_tag="ACGI8A_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HFH0181047.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         4015 a         1094 c         1894 g         3442 t
ORIGIN      
        1 ctaatattag atagtgtatt taaatagata ataataaatt cattatagtt gaataagaat
       61 attatatgag aatattgcaa tttgatatat ttaccattac aataaaagta gttacaacta
      121 gaaaacgaaa taagttaaag taagttggca aattggaggt ttttaaatat gaaagcattt
      181 aaaaagcact tcttgatact ttctttgata tttttactta ttatatcatg ttttaacaat
      241 atactatgct ttgctgatga gtctgaaaat tcaaaaaaaa taagagttgg atattgtgat
      301 gactacggaa taatatcctc atcaaaagag cattcttata caggatatgg ttatgattat
      361 ctaagagaaa tctcaaaata tacaagatgg gaatatgaat ttgtaaaagg gtcttgggaa
      421 gaatgtttag acagactaga aaagggtgaa atagacttac tcggaccttt acagaagaat
      481 gatgaaagaa ataaactatt taattttcct aaactaagtt caggatatga atatagtgca
      541 ctatatacaa aggatagtaa caataatatg ttttatgaag atataagttc ctttaaggga
      601 atgagagtag ctgtacttag aggaaacttt cataatactg catttgagaa gtatagagaa
      661 gaaaataatt tttcagttga atatatatac tgtaattcta ttgatgaatt aattgagtcc
      721 gtcaatgaaa aaaaggcaga tgcatttgtt tgtggaagta ttataaatgc taaaggaatg
      781 aaaatagtta gcaaattttc agtagaacct ttttattttg ctactgcaaa agataaacct
      841 aatttagtaa aagaattaga ttatgcttta aaagaattaa aaataaatga tatgtattat
      901 gagttggaat tatataaaaa atatttcaaa agagaggtca ataattccat agcatttact
      961 cggcaagaaa tgaattttat taaagcaaac ccaaaactta ctatggtata tgattcagag
     1021 tggtctcctg tagagtatta tgatgaagaa agcaattcat ttaaaggtat aagttccgat
     1081 ttaatgtcaa ttatatcaaa aaaatgtggt ataaaatttg aatatattaa aacaaaaaat
     1141 tataatgaga gtcttgatta cataaaaagt ggtaaggcaa ctatgctatg tggctcaatc
     1201 aatgaaaatg ataaggtaaa acgatttaat atgaaattga caaatccata cataaatatt
     1261 ccaatgatta tggtaggaaa attggatacc aatctcaata atgatttaaa tattgcttta
     1321 actagtagtt ataagtctat tgattcttat atagaaaaga gttttgaaaa tgctaagaca
     1381 acatcttata agagtgtaga aagttgtcta aacgctataa atgaaggtaa agcaaactta
     1441 atgatactta gttcttatca gtttgatgaa cttttaagag aaggaaagag tgagaattta
     1501 tctgttatat ctgttttaga tacaagctat gggatgcgta taggggtatc aaataaaact
     1561 gaccctatat tagtgtctat tttaaataaa tcaatagaca aaattactga agaagaatta
     1621 agtgattgta tatattctaa tactattgac aaaccttata aagtaccatt tggtgtgatt
     1681 tttaaagaat attcaataca aattatttct tttgtatgta ttttattctt aattgctatt
     1741 aaatatataa tttataataa aaagaagaaa gaggattatc taaggaagat agcatataca
     1801 gacccattga caggagcaga tagtatagat aaatttaaaa taaattctaa taaactgttt
     1861 gctaagaata atccagaaga atatgcgttg ttttatatag atgttgacaa atttaaatat
     1921 ataaatgata tgtttggata tgatatggga aatgatactt tgatacatat atcaaatact
     1981 attgcaagtg aattaaagga agatgaaatt tttgcaagag tttctgctga ccattttgtt
     2041 cttcttatta agtataagac agatgatgat attaaaacaa ggttaaacaa tatttacaat
     2101 aaggttcaaa tacttagtaa tcctaaaata aactattata aattgatttt agattgtggt
     2161 atatataaaa taagtaaaag tgataatgac ataaatacta ttatggatag agctaatact
     2221 gcaagaaaaa ctataaaagg tggtcataaa aattcatttg ctttttacga taaggaaatg
     2281 cacaaaaaaa tattaaaaga gaaagaaatt gaaaattcaa tggtagatgc tcttaataat
     2341 ggagaattta ttgtttattt tcaacctaaa tatagccttt cagattatca aattataggg
     2401 gcagaggctc ttgttcgttg ggataatcca caaaaaggtc taataccacc aatagaattt
     2461 attcctgtat ttgagaggaa tggttttatt gtaaatattg atttctatgt atttgaggaa
     2521 gtgtgtaaaa agattagaga atggatggat gaaggacaaa aagtagtacc tatatctgta
     2581 aacttgtctc gaatgcattt tgtaaatagt aattttatag aggagtttaa attaattgta
     2641 gataaataca aaattccgac aagacttata gaattagagc taacagaaac agcagtatta
     2701 gataatatag aaggattact tgatacaatg aacaatttga aagaaaaggg atttgtaatc
     2761 tctatggacg attttggtac aggatattcc tctcttaatc ttttaaaaga attgcctgtt
     2821 gacatattaa aactagatag agcatttttt acagaaaaag atgagagtaa taatgaaaaa
     2881 attgttatat ctaatgtcat taaaatggca aaggaactga aaatgaaagt aatatcagaa
     2941 ggtgtagaga ctatttcgca agtagaattt ctaaagcaaa ttgggtgtga tatggtacaa
     3001 ggatatttgt tttctaagcc aatgcctgta aaagagtttg aaaaaatcgc ttttaaaaaa
     3061 gaataaagca atgtgtaaat aatgacatat ataataaagg tgctttaata ttagttgagt
     3121 atactatttt agagcatctt tatttttgta ttaattttta cgattttatt aataaaataa
     3181 atcaaatgag aaaaaaaatc aaaagaatac aataatcact tgcaaaaaat atactattgt
     3241 gatagactta aatcaaaatt aggaattgta aaaagaagga aaaaatagga tgttatttta
     3301 atagattaag atttaaggta ttgtaattta tatataagat aacgattgct tgtagattta
     3361 aattacttta agaggaggga tattatatga tgccattgat attatcaaat acaggtgaag
     3421 aagtagttat aaagaaaatt tcgggaagtg ataagacgag gactttttta aatagtatag
     3481 gttttgtagt gggtacaaat gttaaaatag tttctaagat agatggtaac ttaataatag
     3541 acgtaaaaga tactagagtt gcattagata aaaaaatggc aagtagaata ataatatagg
     3601 gaggtaatga catgaacaga ctgaaggata ttaagtgtgg agagactgta aaagtcaaaa
     3661 agttagaggg agaaggagct acacgaagac gtataatgga tatggggatt acaagaggtg
     3721 ttaatatatt tataagaaaa gtagctcctc ttggtgaccc aattgaggta acagtcagag
     3781 attatgaact atcaattcgt aagtcagatg cagaaaaaat tattgtggaa taataaagat
     3841 taattagagt agggttaatg taattatata atgagaatga ttttctgtat cacaaacagt
     3901 gatgtattta aataataatg gagggaatgg aaatgtcaat aaaaataggt ctcataggaa
     3961 atcctaactg tggtaagaca acaatgttca atgggcttac aggctcatca caatatgttg
     4021 gtaactggcc aggagtaaca gtcgaaaaaa aaggtggaaa attaaaagga aataaagatg
     4081 tggaaatagt agatttacca ggtatttatt ctttatctcc atatacatta gaagaagtag
     4141 ttacacgtaa ttttatgcta gatgataagc cggatgcagt aataaatatt gtagatgcat
     4201 caaatataga gagaaattta tatcttacaa ctcaagtttt agagctaggt ataccgaccg
     4261 ttatagctct taatatgatg gatattgtaa ataagaatgg tgataagata aatattaaag
     4321 aactatctga agttataggt tgcccagtgg tagaagtaac agcagttaaa ggtcaaggaa
     4381 ttatggaggc tgctgaaaag gcagttgaac ttgcaagtag taacaataaa ttaaatttca
     4441 aattaccctt tgttgatgaa agtaaggatg caatagaaaa aattgaaaaa ataatagaag
     4501 aaaagactcc atatatagat gtagaaacac gctggttagc tataaagcta tttgagagag
     4561 atgaaaatgt tatacaaaaa cttgatattt ctaaaacaat attgaatagt attgaagaaa
     4621 taactagaaa ttgtgaagat gaattggatg atgatagtga aagtataata acagccaatc
     4681 gttatgaatt tattagttca ataatttcaa gtataataaa gaagaatcga aaaggtaagg
     4741 aaactgtatc agataagata gataagattg taacaaatcg tatactagca ttacctattt
     4801 ttgcacttat aatgtgggga gtttactata tagcagtaag ttcattgggt actatagcaa
     4861 ctgattggac aaatgatgta ctgtttggag aaattattca aggcaatgta agtaattttc
     4921 tagcttcttt aaatgtagca gagtggttac aaggacttgt agttgatggt ctaattggtg
     4981 gagttggagc agtactagga tttgtaccac aaatcatgct tttattctta ttattatcaa
     5041 tattagaaga ttgtgggtac atgtcacgtg tagcttttat aatggataga attttccgta
     5101 agtttggtct ttcaggaaaa tcatttatac caatgcttat aagttcaggt tgtggagttc
     5161 caggagttat gtcaactcgt actatagaga atgatagaga tagaaaaatg actattatgt
     5221 taactacatt cattccatgt ggagctaaga ttccaataat agcattattt gcaggggcat
     5281 tatttggagg tgcttcatgg gtagctccat caatgtattt cttaggtatt gcaatgataa
     5341 tcatttgtgg aattatatta aagaaaacta gtttatttgc aggagaacca tctccatttg
     5401 ttatggaatt acctcaatat catattccaa gtgcaaaagg tgttttaata catatgtggg
     5461 atagaggaaa agcatttata atcaaagcag gaacaattat atttgttgct tgtggagtaa
     5521 tctggttctt acaatcattt aattggtcat tacaaatggt agatgctgga gatagtatat
     5581 tagctagctt aggaaatata gttgcaccaa tatttgctcc tcttggattt ggaaattggc
     5641 aatcatcagt agcaacagtt acaggtctag tagctaagga aaatgtagtt ggaacatttg
     5701 gagttttatt tggtatatca gatgcaacag agcaagaccc aacacttctt gcttcagttg
     5761 caagtatgtt tacagtagct agtgcgtttg catttatggc atttaatatg ctttgtgcac
     5821 cttgttttgc agctattgga gccataaaaa gagaaatggg ttcatggaaa tggacatgga
     5881 ttacattagg attccaaact ttaacagcgt atataattgc tcttcttata aatcaagttg
     5941 gaagtttagt tttaggtact ggaggaagta ttgctggggc aataatatct atatttatag
     6001 cagtagcagt agtctttgta gttttaactt attcaaataa aaatatgaaa aaagaaaaaa
     6061 tgggtaaatt aagttatatg aaaaattaat tcatataaat aaggagtgat gaatatggct
     6121 acatttatta tagcagctat tgtagtagta ttaatggcac tagctgtaat gtatatggtt
     6181 aaaaattcaa aaaagaatgg tagcagttgt ggatgtggtt gttctggatg ctcaagtagt
     6241 aagagttgtc atagcgctaa gagataaata ggataagagc ctgataaaaa gttaaattgc
     6301 tttattatca ggttcttatt ttttgtataa ataacaaatt tcatatttga agtaggttag
     6361 actatgctgt aattttactg tataattata tataaatata tgattaggag ggattattgc
     6421 taaattaagc aatataagtg aatattatgt ttaaatttga tgttacaaac gagcttatgt
     6481 gtatagcaag gggaagtggt aaattttttg caaaaaaagg tgctatggta gcctttaaag
     6541 gaaattttaa ttttgaaaaa ttgctgttag gtccaagtaa tggcggtgga ttgggaagag
     6601 cattattagg tcatgttcag aggtcattga caggagaaca aatgccaata atggtggttg
     6661 aaggtgaagg tgaaatttac ctagctcaaa atgcttacca tgtagatgta atatcaattg
     6721 acccaggtga ttccatttat gttgagagtg aaaacttatt ggcattttct gaacaattaa
     6781 attacagtgt aaaattgatt ggttcagggg taatatctca aaaaggtctt tttactacac
     6841 atttagtaaa taaaacaggt caaaatcaag atgttgctat aataacagat ggtaatcctc
     6901 taattcttga aggaccttgt tgtgttgacc cagatgcatt agtagcttgg actggaagag
     6961 aaccatatcc taaagtatca aaactatcat ggaagacttt tattggtcag acttcaggag
     7021 aatcttatca tatggaattt gttgaacctg gacaaatagt cattatacaa ccatctgaaa
     7081 gattgtctgg cttaaaaaca gattctacac cttctgatag aatgagttca ggtggattaa
     7141 gacttggcat agattaaatc ttatgatata gcttaattat tatttaagat attaaaaaat
     7201 aaaaagggta gcagggatag atatgttaaa aggagtttta tatgtttgag aaaattcata
     7261 aaaggcttaa aggatattat atttttttaa taattttaat tttatggaag atagtatgtg
     7321 ttttgggtat atggagtagc tatattctgc ctcctcctga aatagtctta gatactttta
     7381 taaaaatgat atatgacggt tctattttct taaatgttta tgccagtgta agaaggatat
     7441 taataggttt tttaataagt tcattgatgg ctataccact tggagtttta tttggagcaa
     7501 ataaaaaaat gtatgaatat ttcaaaccac tatttgaatt tatgcgaagt acacctccac
     7561 tagctttggt gccaatgctt attttatggt ttggtatagg tgaagaatct aaaattataa
     7621 ttattatttt ggcatccttt tttccaatat ttttaaatac attgaaaggt ataaaaaatt
     7681 gtgacaataa attaatagaa gtaggtaaat cttttaattt atctaaaaag agaatttttt
     7741 ataaaataat aatccctaat tctatgctgg atattgttgt tggacttagt ttagggcttg
     7801 ggtatagttt tagggctata attggagcag agcttattgc agcttcttca ggactaggat
     7861 atttgatttc tgatggaaaa gatatgtcta gaactgatgt tgtattggta ggaatttttg
     7921 ttattggatt tttaggtata ctttcagatt atctattttc aaagataata acaaaattta
     7981 gcaaagggaa gcaggttgat ttaaatggtt agaagtggat ttttgctaga gaatatatat
     8041 aagaaatatt tagttgataa taaagagcat ttagttcttg ataatatatc tttaaatata
     8101 tctagtgaag agataactgt tatattaggt gaaagtggat gtgggaaaac aacgctactt
     8161 agaattttag cagggcttga aaatgctact agtggaaacg tatatttttt taataatgat
     8221 aaaaagtgta ctccaaaagt aggaatggta tttcaagaaa gtagacttat gccatggctg
     8281 aatgtaagtg aaaatatact tttacatact gaaaaagata atagaaataa agttgattta
     8341 gacaagtatt taaaaatgat gaagcttgaa aagtttaaaa actcatatcc aaatgaatta
     8401 tcaggaggaa tggctcacag agtaagtata gcaagagcac tttccttcaa tcccgatatt
     8461 ttgctgatgg acgaaccatt tgcagcacta gattacttca caagaagaaa aatgcaaaaa
     8521 gaagttgtaa atatccataa aaatactaaa aaaggtgtag tatttgtaac tcacaatatt
     8581 gaagaagcta tggaaatagc aaagaaaata attgttttta gtaaaaataa aaggattaaa
     8641 caatttagtg tcgaagatga atataataga gatttaacta aaaactatta tataaacttg
     8701 aaaaaagaaa tattaaggga attgggggag ttttaattga ttaaaagaaa caaaaagatt
     8761 ttagcagtaa tgacatcttt aataatggtg ttggttggga ctgtaggatg ttcaacaaca
     8821 aatgataaga aagatgaacc tgcaaaaaaa gaggctagtt tacctaagga aataaattta
     8881 acttatgtaa aatcaccact taatgttcct agtatattag aaaaaaagga tgatttattt
     8941 ggaaaggaat ttaaaaagga caatataaaa gtcaatttcc atgaattgac aacaggacca
     9001 gagcaaactc aagcattagc agcaggtgag ctagactttt tacatgcatt aggaggaacg
     9061 tctgcaataa tagctgcatc aaatggtgtt ggtcttaaaa ttacgaatat ttatagccgt
     9121 tcaccaaaag gatatatgct tataactaat aatgataata ttaaatctat aaaagactta
     9181 aaaggtaaaa aaattgtagg tccaaaaggg actatacttc atcaactgtt gattgctgca
     9241 cttgctaaag aaggctatac tatagatgat gttgaatatg taaatatgga tattcctagt
     9301 gctgcttctg cattagaaag taaaagtgct gatgtagcaa tgcttgcagg tccagtggca
     9361 ttgaaaacga taaattcagg tgccaaaatg attgtaaatg gagaaggatt agtaagtgga
     9421 ataatagtta ctgctgtaag tgatgacttt gctgaaaaat atcctgaatt agtaaaaaga
     9481 tttatgaagg ttcatgaaga aactttaaaa tatatgaatg aaaacaagga tgaagtgatg
     9541 gatgttgtat ctaaagaagt tggtttatct ttagatgaaa caaaagaaat gtattcttgg
     9601 tatgacttta gttctaaaat aacggacaag gatataaaag aattagaaga tacacaggaa
     9661 tttttgatgt ctaatggaat gcaacaaaag aaaataaata ttaaagatat gttgtacaat
     9721 caaaattaga atactttaaa atattaaagt ggaaaataca cccttattta ttatggaaac
     9781 tgtatggtaa aagagggtgt atttttatta ttatacaaaa acacaattaa aaacagctta
     9841 ctattttatc tacacttatg tagtctagta attttctcat tttcttacgg tgaagtaaaa
     9901 acttcgaata tgtgtcctcg gattgttttg tgtcaccata aactttccaa tatttaatca
     9961 tagaaacttc ataattacta tatcccataa atccaatcac atctttaaga ggatatccta
    10021 aaaaaatacc tatttcatca ggaaaaacat cactcttaag tttatctact aaatgctcta
    10081 aataagcatt aacattcgta ttttgtttgt atcctaaaaa ctttaaaaag tttactgttt
    10141 ttttacattt taatttttct gataatgctt ttttatttac aaataagatt tttaatccct
    10201 ttttttcttt atcaataact ataaaatcta ttttcttaca cttatataga tatttataaa
    10261 tatcattaat tttttcctct ctatttacat cagtaaacga aatatttatt atttcagcag
    10321 gtttagagcc taaaaagact ggacctaaaa cttctaaaag ccattttata tatgatgatt
    10381 caattttatt tttacaacaa aattgtgaat tacaacaaga cataaaagat aacctccttc
    10441 aaatg
//
LOCUS       DAYATN010000133        10203 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.133, whole genome shotgun sequence.
ACCESSION   DAYATN010000133 DAYATN010000000
VERSION     DAYATN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.133"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            417..1577
                     /locus_tag="ACGI8A_003066"
     CDS             417..1577
                     /locus_tag="ACGI8A_003066"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFH0181048.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1579..2709
                     /gene="glgD"
                     /locus_tag="ACGI8A_003067"
     CDS             1579..2709
                     /gene="glgD"
                     /locus_tag="ACGI8A_003067"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HFH0181049.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2738..4180
                     /gene="glgA"
                     /locus_tag="ACGI8A_003068"
     CDS             2738..4180
                     /gene="glgA"
                     /locus_tag="ACGI8A_003068"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFH0181050.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4194..6614
                     /locus_tag="ACGI8A_003069"
     CDS             4194..6614
                     /locus_tag="ACGI8A_003069"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HFH0181051.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRVLL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6619..8484
                     /locus_tag="ACGI8A_003070"
     CDS             6619..8484
                     /locus_tag="ACGI8A_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFH0181052.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIRIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            8729..9382
                     /locus_tag="ACGI8A_003071"
     CDS             8729..9382
                     /locus_tag="ACGI8A_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0181053.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPMEEMKKEKLL"
     gene            9478..10077
                     /locus_tag="ACGI8A_003072"
     CDS             9478..10077
                     /locus_tag="ACGI8A_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFH0181054.1"
                     /translation="MYPNKEIAIEELEVAGKMNPGPWIKHSYNVANVAKLIAEQCENL
                     NSEKAFVCGLLHDIGRRTGISSVRHIIDGYDYVISKGWDEVARVCLTHSFPVKDIEAD
                     IGNKDISKEQYDFINHYLNNLEYNDYDKLIILCDALADANGFCILEKRFVDTTRRYGI
                     YPFSVERWNKTYEYKEYFEKIINKSIYTLLPNIEKSIYY"
BASE COUNT         4089 a          998 c         1865 g         3251 t
ORIGIN      
        1 gtatcaatta aaaaataaag ttgattaata attacttgat tttccttgga taattattta
       61 acaaatatta gattatattt tgctattacg aatttaacaa attaaaaaat aagcatgttc
      121 atatgacaca aaggatttat aaaaaatgtt tttatattta taaaataaaa tttgtattat
      181 tttttaaccg ataattgaat attaataaat actatagtgc ttatggaaaa taatgtatac
      241 gtcggtaagt ccattatggg gtatacatta tatcaattgt atagttgata ttattttatt
      301 tttattaagg tattatttaa gcaacattta tacacgacat ttattgactg ataaagttta
      361 taaaagtaaa aatgcaatta taaatattaa ttactagatg aggggagtgt cattaaatga
      421 aaaaagagat gttagctatg attttggcag gagggcaagg ttcaagatta ggtgttttta
      481 caaaaagaat tgccaaacca gctgtatcat ttggaggtaa gtatagaatt atagattttg
      541 tgttaagcaa ttgttcaaac tctggtattg atactgtagg tgttttaact caatatagac
      601 cgttgatatt aaattcacat ataggcatgg gaagtcattg ggatttggac agaataaatg
      661 gtggtgtcta tgttttacaa ccatttatga atgaaaaaga aggaaattgg tataatggta
      721 cagcacatgc gatatatcaa aatatggact ttgtagatac ctataatcca gaatatgtat
      781 tgatattatc tggtgaccat atatacaaga tggattatag caaaatgtta aaatttcata
      841 aggaaaaagg ctctaaagct acaatagcag tgattgaagt tccttgggat gaagcttcaa
      901 gatttgggat aatgaataca aatgaagatt caagtatcta tgaatttgaa gaaaaaccaa
      961 gtgaacctaa gagcaatttg gcatctatgg gagtatacat atttgattgg aaaatgttga
     1021 gaaattattt taaggaagca gaaaaaaatc cagaaataaa ttatgatgat tttggaaaaa
     1081 atttaatacc taaaatgtta gaagataatg ttggaatgta tgcttatcca tttaaaggat
     1141 actggagaga tgtaggaact atacaaagtc tatgggatgc taatatggat ataataaaat
     1201 ctccagaaac acttgattta gcagacccta agtggaagat atatacaaat actatggcaa
     1261 tgcctccaca atatataggt aaaaatgcaa atgttcatag atcaatgata gctgatggtt
     1321 gtagaatttt aggtgaagta ggtaactctg tattatcaca tggagttgtt gttggtaaag
     1381 gaagtaaagt aattgattca gtaataatgc caaatgttgt aataggtgaa aatgtaacta
     1441 tagaaaaagc tatgataggt gagtgtgcaa ctataaatga taatgttcag ataaagaatg
     1501 taaataatga aataaatgta gtttctgagt atgaaaatat cgaacctaga tgcgtattaa
     1561 tagaaggagg tctttaagat gagaaatgaa tgtttaggga taataaattt aaacaaaaaa
     1621 ggagacccag caataaacaa acttaactat ggaagaccaa tagcatctac accaatagct
     1681 ggaagatata gaataataga ctttgcttta tctaatatga taaattcagg aatcactaaa
     1741 gttggaatat tcgcaaaaga aaaatacaga tcattaacag accatatagg tagtggtaaa
     1801 gattgggatt taagtagaaa aaaaggtggc ctatcaattt ttagtccaga aaacactaag
     1861 tatagaaata cttactcaca tagagaaggt gatatatata ctatcttagc taatctagat
     1921 tatatagaaa agagtgaaga agaatatatt ttaatagctc caagctatat gctatgcaat
     1981 ttaaattatt ctcaagcatt ggaataccat aaaaaatctc ataatgatat aactataata
     2041 tataaaaatg taaataatgc aaataaagat tttgctggta acttaactct aaatttagat
     2101 agtaacaata gggtaataaa tgtaggaaat aatttaggta agttccctag agcaaacata
     2161 tgtatggaga catatattat gaagcgtgaa gattttgttg aatgtatata taacattgta
     2221 aataaaggca attattgtta tttagaagaa tttattattg aagaagcaga aaacatgaaa
     2281 ataggagcct atgaatatac aggatatcta aaatgtgtaa attctgttga atcatatttt
     2341 gaaatgagca aagatttatt aaatttagaa gttgcagatg aattattata ttctgaaaga
     2401 aagatattta caaaagaaaa gaatgaatca cctacaattt atacagatag tgctaaagta
     2461 gaaaatagct ttatagcatc tggttgttta atagaaggta ctgtaaaaga tagtatcata
     2521 tttagaaagg taaatgttga aaaaggaaca gttatagaga actcaatagt aatgcaaaat
     2581 tgtgtaataa agtcaaatgc aaaactatat aatgcaatat tagataaaaa tacaagtgta
     2641 tcaaatggga aagaattaag aggagatgaa aaatatcctc ttgtagtgga aaagaataca
     2701 aatatataaa taaatttttt agaaggggga atgactaatg aaggtttttt atgtaacagc
     2761 agagtgttgg ccctttgcaa aaactggagg attaggagat gtttcttatg cattgcctaa
     2821 agaattaaaa aaagaaggtg tagatgtaag ggtaataatg cccaaatatt caactatacc
     2881 aagttattta aaagatcaac taaaagaaat agctgtgttt agtgtacgtg ttggatggag
     2941 aaatcaatac tgtggtcttt tagaaatgga attggatggt gtaaaatttt actttataga
     3001 taatgaattt tattttagaa gagaagatga aagaaagagt atctatggat atggagatga
     3061 tgcagaaaga tatactttct ttacagatgc agttttagaa gctataagta gaattgattt
     3121 ttatccagat gtaatacata taaatgattg gcatacaggg atgttaccat taattctaaa
     3181 agaaagatat gcaacccttg aggggtataa aaatataaaa actatgtata ctatacataa
     3241 tctccaatat caaggtgtat ttgataagca tgttttatat gatatattgg acttaccaca
     3301 aaaatacttt gataatggag atatagagta ttatggctca ataaatttta tgaaagctgg
     3361 tataaatttt gcagataaaa ttataactgt tagtcctact tatgcaaatg aaatacaaac
     3421 atctttttat ggcgaacaat tagatggatt acttagaaaa gagtcaggaa aattaaaagg
     3481 tatattaaat ggtatagatt atgatttaaa tgaccctgca aaagataaag atatatttgt
     3541 tcattatgat gtagactcaa ttgacaaaaa agtagaaaat aaattgagat tacaagatat
     3601 attgggacta aaaaaagatt catctattcc gttgataggt atagtttcga gattagtttc
     3661 tcaaaaagga tttgatttaa tagcatacat gatgccagaa cttatgagag aagatttaca
     3721 gattgtcgtt ttaggaactg gagaacatca atatcaatca atgttcaact attacgattc
     3781 taatttttca gataaagtat cagctagaat tacttttaat gcatcattgg ctcaacagat
     3841 atatgctgca agtgatatgt tcttaatgcc atcattattt gagccttgtg gtataggtca
     3901 aatgttagct atgagatatg gttctttacc aatagttaga gagactggag ggcttagaga
     3961 tacagtaaca ccttataata aatttactgg agaaggcaat ggatttagtt ttaaaaatta
     4021 caatgctcat gagatgtttt tctgtttgaa aaatgcaatc aaagtattta aggacaaaga
     4081 aaaatggatt aaattagttg aaaatgcaat gaaaacagat aatagttgga aaaaatcagc
     4141 taaggagtat atagaaacat atagagacat atgtgattaa aggagtggtt tttttggtaa
     4201 caataagcaa aaagaaattc aagcaaaaat ttgaagtaaa aatgtatagc ttatatgcac
     4261 aatctatcaa ggaagctact gatgaacaat tgttaaatgt attatgtagt ctattgaaag
     4321 atgagatagc aaaaaaatgg gttgctacaa agttagataa gaaaaaagaa gtttattatt
     4381 ttagtttaga atttttaata gggagacagt taaaatctaa tttattaaat ttaaatattg
     4441 aagaagaagt tagagaaggt ttatctgaat taggaatcaa cttggatgat ttaattgaag
     4501 ctgaagtaga cccagcctta ggaaatggtg gacttggtag attagcagct tgctttttag
     4561 attctatggc atctttgaac ataagtggtc aaggatatgg aataaggtat aaatatggtc
     4621 tgtttgaaca aaaatttgta aatggttatc aggtagaagt gcctgataac tggcttacag
     4681 aaggacgata tgcatgggaa actgtaagac caaatgaagc aacaatggtg aagtttggtg
     4741 gtgaggtaga attaatcaag gaaggtagtc atttaaaagt aattcataaa aactaccttc
     4801 ctgtaatggc aatgccttat gatataccaa taatcggata ccaaaaccaa tgtataaata
     4861 ctttaagatt gtttaagagt gaaataccaa agagagattt tggtgaactt acctcaaatg
     4921 ctctaaatta ttctggtagc tatgaagagg ctttaaaaca taagtattat actgaggaaa
     4981 tatcacaggt attatatcct gatgattcaa actatgcagg taagttgttg aggttgaaac
     5041 aagaatactt ttttgtaagt gcagggatac aagatataat aagaaaatat aaaaagaaca
     5101 aattaaatat taataattta ttcgataagg tagcaattca tataaatgat acacatccta
     5161 ccttatgcat accagaactt atgagagttt tgcttgatga agaaaattta tcttgggatg
     5221 aagcatggga aataactaaa aaaacagtgt cctatactaa tcatactata atgtcagaag
     5281 caatggaaaa atggcctgta agtatgatga aagaactatt accaagaatt tatatgataa
     5341 tagaagaaat aaatagaaga tatgtagagg aattaaataa taaaggatat gaccaagata
     5401 gaataaaaag aatgagtata atagactgtg acaatatcaa tatggcaaac ttatgtattg
     5461 tcacaagtca tagtgtaaat ggagttgcta agcttcatac tcatattctt gaaacagagg
     5521 tattaaaaga tttctatcaa gatgaaccaa ataaatttaa taataagact aatggaattg
     5581 cacataggcg atggcttata agttcaaatc ctcaacttag caatttaatt acagatttaa
     5641 ttggtgattc atggaagaaa gatacattac aattaaaaaa tattgaaaaa tttaaaaatg
     5701 attcctctgt tttacaaaga cttgatgata taaaatacaa taataaagca aatctagcaa
     5761 agtttataaa agataaatac gatttaaatg tagacccaag ttcgatattt gatgttcaag
     5821 ttaagagatt gcatgcgtat aaaagacagt tactgaatat atttaatgta cttcatatgt
     5881 atcatgaatt acttgataat cctaatttga atcttgaccc aagaactttt atatttggcg
     5941 caaaagcagc accaggatat tatttggcta aatgtataat taaatttata aattctgttg
     6001 caagtactat aaataatgat gttagagtaa aggacaagtt aaaagtagta tttttagaga
     6061 attatggagt atcattagca gaaataataa taccagcagc aaatgttagt gagcaaattt
     6121 caactacaac taaggaagca tctggaacaa gtaatatgaa gtttatgatg aatggtgcta
     6181 taactttggc tacacttgac ggtgcaaatg tagagatatg tgaacaggtt ggtaaagaaa
     6241 atatgttttt atttggactt agtgcagaac aagtactaaa ttataataaa tatggtggat
     6301 attcttcact tgacctatat cattctaata tggatataaa aagggtagta gatgatttaa
     6361 taaatggatt tattcctaat cttggcgaag aaggaagaag tatttataat tcactcacta
     6421 cttacaatga tgagtatttt gtattgagag attttgaaaa ttatggtcaa gctcaagcag
     6481 atataaacag gttatataga gataaagaaa aatggaataa aatgtcttta gttaatatag
     6541 ctaattctgg atttttttct tctgatagaa caatttctga gtatgcaaaa gacatttggt
     6601 ttaaaagggt gtgattaaat gcaaagtgta atagaatata actcttggga taaaaatttt
     6661 aaggctccgt ttggagcttt aaaatttgat gaggaattga ccatttgtgt taaagtaaat
     6721 gagggataca atatcaagtc tatttcccta gaaataaata gagaagaaga aatgagaaca
     6781 ataactttaa atgaagaatt agataatgat aaacttggaa agtgcttttg tggtaagatt
     6841 gaaaagtttg atggtacagg tgtttatttt tattacttta aagttgatgt tgaaatggat
     6901 ggtcagataa agactttatt ttatggaaaa aacagagata atggatattc ttgtgaatat
     6961 aattatagtg atattaataa atatcaaatc actgtttaca aagattttaa agtaccaaca
     7021 tggtacaaag aaggaatact ttatcatata tttgtagata gattcaataa tggaaatcgt
     7081 agtggtaagg ttgataatcc taaaaaaaat agtttcatat atggaaattg ggaagataca
     7141 cctatgtaca taaaggatag tcaaggggat gttataagat gggatttcca tggtggaaat
     7201 ttaagaggga taatcaataa acttggatat ttaaaaaaat taggtgtaag tatactatat
     7261 ctaagcccta tatttgaagc ctctagtaat cataagtatg atacaggtga ctataaaaaa
     7321 attgacccaa tgtttgggga tgaagataca tttaaagaac ttattgataa agcaaaagaa
     7381 aaaggtatat caatagtatt agatggagtt tttagtcata ctggagcaga tagtaaatat
     7441 tttaatatgt atggaaacta taatagttta ggggcatatc aatctaaaga atctccatat
     7501 tattcatggt atatgtttga agaatttcct caaaaatata aaagttggtg ggatgttaag
     7561 acattgccaa atataaatga attagaacat agctatatgg attatataat atatgacaat
     7621 gatagcgtta taaataaatg ggtaaatatg ggtattaagg gctggagact agatgttgca
     7681 gacgaattac caactaagtt tataagagag ttaaaaaagg aattaaaaaa ggcagatgat
     7741 gactcaattc ttataggtga agtatgggaa gatgcatcaa ataagattag ctatggtcag
     7801 agaagaagtt atttattagg tgaagaatta gattctgtta tgggttatcc ttttagaaat
     7861 aacatgtttt ctttcttaaa aggagaaatt aattcatatg aactttgtaa tagatatatg
     7921 cagattaaag aaaattatcc aaaagaatca tttaggtcta atttaaattt aattggaaca
     7981 catgatgtta caagagctaa aacggaatta aatgatgatg tagatttagt gaagcttgct
     8041 gtagctatac agatgacatt tgaaggagta ccatatatat attatggaga tgaagcaggt
     8101 ctttgtggag gtgttgaccc agataataga agaacttatc catggaaaaa tgaagacgaa
     8161 gatatgctta atttttatag agatgttata agaattagaa ataagaataa acttttaagt
     8221 tctggaaata cagaatttat atatactaat aatgattctg tattttcttt tattagagtt
     8281 aatgaaaata atgatagaat gttaatttta gtaaatagaa gtgaaaatgt ggaaagtata
     8341 tcattatgta tagagagtag ttttattgag gaaataccta taaagtatag tcttaaaaat
     8401 gcaaatagta ctatacaaat tgaaaataat gagttgaaag tagatttaga ctctaaatca
     8461 tttagaattt ttagagtaaa ttaaatatct ttttttagaa aaataaatag aaaataaggt
     8521 gaatgtggaa agagttttgt tttatcaaaa ctttttctac tttttttgta taaattatca
     8581 taagaatttt agtatttcaa aatattacaa agaagtataa aaaatatcat aattgtgata
     8641 ttatataaat tagtaagata aaactgaact ctctaccaaa gttgtaggga gatttaactt
     8701 ttataatctt aatatggagg aacgtcttat gaaggtagat ccacttacaa ctcaagtata
     8761 tgactatata tcaaagaaaa tccaaaatgg agaatatgaa gctaatcaga gaattactga
     8821 atctgagatt tgtaaatcta tcggagttag tagaacacca gcgagagaag cattaactag
     8881 gttagcaggt gaaaatttac tagaaaaaat acctaataag ggatttgtag taaaagaatt
     8941 tcaggaaaag gagaaattag acacctattc cgtaattgga gtattggatg cgttagccgg
     9001 ttcttcagca ttacaaaatt taactgaatc agacctagta aaaatggaag aattaacaga
     9061 aatgatggcg gtctctataa agtataaaaa ttacaatagc tatttaaaat taagtaatga
     9121 atttcacgat atatacataa ctaaatcaga taatcaggtg ttagtaaatc tattgaactc
     9181 tttaagatat aattttatgt caaagtcata tactagtgat aatgaagatg agttatataa
     9241 aatgttgaca tacagcaata atcaacatat tgaagtagta aaatctatga gggagaatga
     9301 tttagaaaat gttgaaagaa ttttgagaga acattggaaa acgataccta tggaagagat
     9361 gaaaaaagag aagcttttat aaagtttctc tttatttttt ataccatttt atcactattt
     9421 ttttgaatta ttaagtagaa aataataatt aatttaaact tgaggaggaa aactaatatg
     9481 tatccaaata aagaaattgc aatagaagag ttagaagtgg ctggcaaaat gaatcccggt
     9541 ccatggataa aacattctta caatgttgct aatgtagcta aattgattgc agagcaatgt
     9601 gaaaatttga atagtgaaaa ggcctttgta tgtggattat tacatgacat tggaagaaga
     9661 actggtattt cttctgtaag gcatattatt gatggatatg attatgttat ttctaaggga
     9721 tgggatgaag tggcgagggt atgtttgaca cattcttttc ctgttaagga tatagaagct
     9781 gatattggaa ataaagatat tagtaaagaa cagtatgatt ttatcaatca ctatttaaac
     9841 aaccttgaat ataacgatta tgataagttg attattcttt gtgatgcatt agcagatgca
     9901 aatgggtttt gtattttaga aaaaagattt gttgatacaa cacgaagata tggaatatat
     9961 ccattttcag ttgaacgatg gaataagact tatgagtata aagaatattt cgagaaaata
    10021 atcaacaagt ctatatatac attgttaccc aatattgaaa aatctatata ttattaaatc
    10081 ctaagttaat aatttaatat ccagtatcac cagcagttgg tcattatgat tgactgcttt
    10141 tattatgctt atattaaaaa aatacactat ggtttgtgta tatcaaagtt atatggaatg
    10201 aaa
//
LOCUS       DAYATN010000134        10086 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.134, whole genome shotgun sequence.
ACCESSION   DAYATN010000134 DAYATN010000000
VERSION     DAYATN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.134"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            162..1061
                     /locus_tag="ACGI8A_003073"
     CDS             162..1061
                     /locus_tag="ACGI8A_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020397798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFH0181055.1"
                     /translation="MSIVLNMPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKN
                     NKINFEAVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLV
                     GNYIAERYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVN
                     QKLSEWTSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLF
                     RYGGDEFLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDI
                     ETLISKADSLMYENKLKNKSTLN"
     gene            1270..1419
                     /locus_tag="ACGI8A_003074"
     CDS             1270..1419
                     /locus_tag="ACGI8A_003074"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0181056.1"
                     /translation="MTTVAEGIETSFQKDFLQEINCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(1679..4246)
                     /locus_tag="ACGI8A_003075"
     CDS             complement(1679..4246)
                     /locus_tag="ACGI8A_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HFH0181057.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYMSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(4249..4854)
                     /locus_tag="ACGI8A_003076"
     CDS             complement(4249..4854)
                     /locus_tag="ACGI8A_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0181058.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLNGIMNDGGE"
     gene            complement(5228..6688)
                     /locus_tag="ACGI8A_003077"
     CDS             complement(5228..6688)
                     /locus_tag="ACGI8A_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HFH0181059.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRGNIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            7050..7628
                     /locus_tag="ACGI8A_003078"
     CDS             7050..7628
                     /locus_tag="ACGI8A_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0181060.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            7919..9169
                     /locus_tag="ACGI8A_003079"
     CDS             7919..9169
                     /locus_tag="ACGI8A_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0181061.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            9330..9899
                     /locus_tag="ACGI8A_003080"
     CDS             9330..9899
                     /locus_tag="ACGI8A_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFH0181062.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3377 a         1733 c         1477 g         3499 t
ORIGIN      
        1 cttatttaaa gtttcatcta attataaatt ggacaatttt tatgttgatt ttattcatac
       61 aaattaaagt aacttataaa atctccacaa taaaaaacat tttttattca ctaaactgct
      121 ccatcattgg tttagcaata aatatatttt ttaggtcttt aatgtcaata gtgttaaata
      181 tgccagcata tttatttgat agtaattcta ttgcaaatat ttacagagta tttcctatat
      241 catttagctt tatagttaca ggactatatt tcagttataa aaataataaa aataataaga
      301 ttaactttga ggcagtttta gaggacaaaa aaaacttaat tttcttattg agccttatgt
      361 ttggaatgta cttgtatcta tgtttgagtt tgtcaatata ctatactgtt ggaaatgtat
      421 tgattttaaa attatatgga atcaagtctg ccataatagt tttggtaggt aactatattg
      481 cagaaagata tagctttaga attactcaaa taaaaggtta tgatattaaa aatcaaatag
      541 aatatgtaga aagtgtaaag acagaatatg agattaataa attgaatgat attgtatata
      601 cagatttact gacaggtttc tacaatagac cttttgtgaa tcaaaaacta tcagaatgga
      661 catcaaatca ctacaaattt accttatgtt ttactgattt aaatagctta aaacctgtaa
      721 atgataaatt tggacaccaa tttggtgata aatacatatc cactactgct caaataataa
      781 aaaaacttat aaatgtagaa aattccttac tatttagata tggtggagat gaatttttat
      841 tccttttaca gaatacaagt tgtgtagaag cagaaaagat aatgattaat ataaattatg
      901 aattatcaaa attaagtaat actgacgagt atccttatgc tctttccata agctatggat
      961 tagttgagtg ggatggaata tccgatattg aaactttaat atcaaaggca gattctttga
     1021 tgtatgaaaa taagcttaaa aataaatcta ctttaaacta gctagttaag atattctaca
     1081 aaatatatgt tataagagag tactaagaat atcaaaatat atagattttg caaatattga
     1141 atacagttta ctaatgaatg cactggatgt aagtgttagt aaacacctat tagatgaaca
     1201 ctttactgtt gtttgggcta atgaccgtta ttatgaaatg ttcagctata cctagctaaa
     1261 aagttaaaaa tgaccactgt agccgaggga atagagacca gttttcaaaa agatttcttg
     1321 caagaaataa actgtgatat gttacaagga tacgtgttct ctagaccttt accaataaaa
     1381 gactttgaaa aacttatgtt tcaaaactcc tccaattaac aatataaaat tgcttattat
     1441 aagtgatatt attgtatcaa caaataaagg aattgtctca aaattgattt tttctaatca
     1501 aatttttatt ttgagacatt ctcatttttt tgcataagaa aatacccacc aagctacggt
     1561 ttttcttacc gctgttcggt gggtattagt agtattatga aatctgaaaa ctttaagtat
     1621 tactacttcc gctaacgcaa tcacaaaggt cgaaagcttc aaagcgaaaa taggcttttt
     1681 actcatcaaa cgtcaccgta cctgccaacc cgtccaccgt tatcatttgt ccgctcttaa
     1741 tagttcgtgt ggcaatgtgt gtagccataa cggctgggat gccatattca cgtgcgacaa
     1801 tgcttgaatg gctaagagga ccaccaatgt ccgttacaat cgcacttgca gatgaaaata
     1861 atggtgtcca tgctggagtt gtggtaactg caaccaaaac acttccaggt tgaaagcttt
     1921 caaaatctgc tggactattc agtacacaag ctggagccgt cacaacacca gtactggtac
     1981 caatgcccgt caacacaatt ttcccatctt tctgcttctg ttgtgcttgt gatatagttt
     2041 ttttgttttt ctctggcaac ttggatggtg acatataacc catgtatttt ttaagttcag
     2101 cttttcgaac aggtattatc ccgttcatat cagatagaga cttgttttta tccaactgct
     2161 ctataagtac ctccaattcc gattttgtga gccaataaat atcatctaaa tgtgaaatag
     2221 ctcctcctcg tatgaatcgt tctgagattt cctgaaacat acaacgaatc agaggatgtc
     2281 ccattcccat caaataaata gcattttcac gcataggaga ggtttcctgt gcccaatgga
     2341 gcaacttaaa aaatagtttt ttacgcaatc cacctatgtg ttgtaaaatc tcttcttctg
     2401 catgttttct acgcttttca aacgtggtct ggcgtaaaaa aggactttcc ccttttcctt
     2461 ccacaaatgt ttttacggat tcaaaagttg gtgtaagtgt ttcctgcggt gtggaatgcg
     2521 caaaatcaaa ctcataagcg gtacgaccaa actctttaaa gtacttattg attcgatttt
     2581 tccattctat ccatacttcc tgtgaaactc caacaggcac aactgaagac atgaaatctt
     2641 ctgctatctt tattgcagga ctgttttgca gataaaggtt gatgctgtta ttttgcttta
     2701 cccattctga aataccccac aactcctttt ccgattgcag aacaatcgta tcaaacccaa
     2761 gcaggaaaac ggaagtatcc gttataccag cacgtcgaac tgctccttga aataattttg
     2821 taaataatgt ttcactgata gaagcagcag gcaatgtaag ctgaatcctt gtgaaataaa
     2881 tacatgcggc ataaaatatt gtctgaatac cctccataat ttgatgggcg ttcatagtat
     2941 gcaggggctt ttcttcccat tgtttaatag catcctcata tccttttcgt gccgtttccc
     3001 agcgtgcaac gctgttcata agtgtcctac gtaaagagcg tggggaaagg gatttgatag
     3061 caatcaaaag tggctttgat ttagcagaaa gataaacata tccattaata accgtatatg
     3121 ccccgttttt tggtaataat tttttcgcac ttttaccaaa catatctatc cataaaagct
     3181 ctgatgcacg gtttacgatt tcaagaccaa gtgtggcgaa caaaggtgta acaggatttg
     3241 gcaaatgttc tgccaaactt ccctttgtgt atacaacatc ctgttctggc aatacccatt
     3301 ctggtggcag aacagtaatt ggacgagcct gaacaatgta caatttatct ttttccagtg
     3361 cccactctac atccataggc atttgataat acttctcaat tttctttcca agctgtatca
     3421 gttgcattac ctgattacga gtaagagcat atttttttct caacctttca ggaaccatgg
     3481 tttcttctgt tccttctgaa gtacgaaccg tcataatctc tttatttgcc acttcatacg
     3541 atataattcg ttcggagtcc ttatccacta cgattgtatc gggtgtaact aagctagaaa
     3601 ccaccgcttc accaagcccc catgcagcgt taataatcat ttcgctacgt ctaccattga
     3661 ttgggtttaa cgtaaacata ataccagatg aatcggaaaa tgcaagcttt tgtactacaa
     3721 cagcaagtgc gacaatctcg tgctttatat tattcttcac acgataagca atagcacgag
     3781 ctgtccataa ggaagcccag catctcttta cagagtcaat aactttatct tcaccttgta
     3841 tgttaagata agtttcctgc tgacctgcaa aagaagcgtc aggtaaatct tcagcagtcg
     3901 cagacgaacg gacagccacc gatatgttcc ctaatccagc ataagccatt ttaattgcgt
     3961 ccgacacttc ctgtggcatt tctccattat ggaaaagctc ccctattttc ttagatacat
     4021 cttcaagttg gctggtatta ttggaatcaa taccatctag cagtttatta attcgagact
     4081 gaatatggtt cgtctcaaca aaaatctggt atgaagcagt cgtaacatga aaaccgtctg
     4141 gcacagggat gcctgctgtc aatagttttg ataaagacat cccttttcca ccaacaattt
     4201 caatggttgc ttgtttatgt tccagtggca agacataata aatcataatc attcacctcc
     4261 atcattcata attccgttca gaaatatatt cattgtttca tccaacattt tttccgaact
     4321 atccaatggt cgtgacgtgt agctgattga taacatggag ttaattaaca aacgtgtcac
     4381 aagagcggta tccgttttgc gaaagatgcc tgtggtcaca ccattatcaa ttaccgattt
     4441 tataatatct tgatatgcat aacgagcatc tttaagtctc tttcgatact cttcttctaa
     4501 ataatcagat tctgtattta accacatcag tacagttcta tactgctctg gaaatctaag
     4561 atgattaagc atgatttttc taaggcactg ttgtggtgaa ggttcaccat caataatcct
     4621 atgaacttgt tggatagttt tttctatttc tttctcaact gcatatacaa caatctcgtc
     4681 tttcgtttta aaaaaatcgt atagacttga ctttcccatg ttggctaaaa ctgcaatttc
     4741 tctcattgat gttttttgga atccattttc agccatgaga ctcaaagcaa cagcaactat
     4801 tttttctcgg cgttctgctt gttgttcggg tgtaagcaca attccttttg gcatagtgcg
     4861 atacctcctt ttagcgacca ctggtcgttt cttttttatt tatcatacag caaccaacgg
     4921 tcacttgtca aggttaattt ttttcattgt atatggtact cgagataaaa atgttcattt
     4981 tagaaattac tctatttaag tagaaatagg cttcatcatt agaaagcaag actataaatt
     5041 tgagatattt atcatgttca aatttcatca atatcaaagt ttaaaatatt aataaaaaaa
     5101 gaattactaa gaaataacat ataaagattc aaaaaaggag ttgaacttaa aattcaatta
     5161 caaaaaatca aatttaagac aactccttta ttctttatct ctactattct ttatctctat
     5221 ttatttatta caactcccta acatttacca ctactgcctt aggtctagtc atagcctcaa
     5281 ttgaatactt aacaccttga gttcccatac cagatgcctt tacacctaaa aatgggaagt
     5341 ggtctggacc tctttcagtc ttattattta tttgaacagt accaacttca agtttatcag
     5401 ctatataaaa tgccttatca atatcacttg taaatactga tgattgaagc ccatactctg
     5461 attgattagc aatttcaatt gcttgattta tatctttaac tcttataatt ggaagtactg
     5521 gaccaaatgg ttcttcccaa gcaattttca tatcaacatt tacattatct aacaatgttg
     5581 gatagaataa attatttttt ctaacattgc ctgttattaa ctttgctcct ttatgtaatg
     5641 catcatcaac caacccttga acaaaatcag ttgctttatt atctataaga ggagtgataa
     5701 ctacttcatc cattggattt ccaattgtca acttctctac caatggcttt atttttccga
     5761 ctaatttatc cgccacactc tcttgtacta aaattctctt tactgccgta catctttgtc
     5821 ctgaatatga atacgctcca gatactatat tcttagctgc aaaatctaag tctgcatctt
     5881 ctaatactat agccgcatct tttccaccaa gctccaataa taatggtacc attccagata
     5941 ttttagatat atgctgacca acttcagtac taccagtaaa gttaataaaa ttaattcctt
     6001 tatgcgttac aacataatcc cctatctcac tacccctacc tgtaatagta ttaagtactc
     6061 catctggtat cccagctttt ctcataattt ctaccacatg aagtgcgcta atagcccctt
     6121 gtgtagcagg ttttaatacg actgcatttc caccaataag tgctggagca atctttgaca
     6181 ttgataaatt tacaggataa ttaaaaggag atatagctaa aacagtacct aatggaaccc
     6241 ttgaaacata agacatctta tttcttgttc caccagggaa attatctcca ctaatggctt
     6301 ctccctccat attttttccc acatcagctg tgtatcttaa aaaatctgct gttctctcaa
     6361 cttctgccct tgcagatttt atgtctttgg ctatttcaag taccagtata ttagctatct
     6421 catctatatt ttctaacaat aaatcagcag ccttatgaaa tatattgcct ctcttgtata
     6481 caggcatttc agcccatact tttatactct cttttgtgtt ttttataacc tcatctacct
     6541 catgttttgt catagattgt actttcccaa caagagaatt atctattggt gagtaaattt
     6601 ctattggctt tctcgaatta gattctaccc attttccatt tattaaattt ttatatacat
     6661 tttcttttgt tttaagttca ttaaacatat ttttattcct ctctctgata taactttgtg
     6721 tatttaaatt atagccattt attcgttatg taaacatttc tttttttcat aaacgtttac
     6781 ctataaaaaa attttaagtt aacgtttttc tacattcttc tattttaata acatactcgc
     6841 tccaaatcta tataggtcac actttaatac taatatatca atataccaat ataccaatat
     6901 accaatatac caatatacca atataccaat ataaatatta taaacataaa tcacttgaca
     6961 aatttataag tagacaatat tatatatcta tgactcaaaa tatttttaaa gtcatatata
     7021 tcatatatag acagaaagga ttgataaaaa tggctaaaag ttttacagaa atagaaaaag
     7081 acaatataag aaaaagacta atttcagaat gtgaaatcaa ctggtctaaa tatggataca
     7141 aaaaaactaa tattgatgaa ctttgttcaa aatcaggaat tgcaaagggt tctttctata
     7201 cattttttga ttcaaaagaa aagttatttt ttgaagtatt aaatggaatc caaaacagac
     7261 ttatatcatc agtagatgaa attttaagta acatgcctcc aaaagaaggc tttgcaaaat
     7321 ccttaaaaat gttatatcga ttatatgata aaaatccatt tttatattca ccaaataatc
     7381 ctgatttcat cagcctaata aataaactac ctcaagatat gtttttacaa cttgagtcca
     7441 ataacataca aagtttttac gacttaattg agagacacaa tctcaagttg aagatagaaa
     7501 aagaaaaagt ttttggtgtt tgtaatgctc tactatcaag tctgttggta aaagaaaata
     7561 ttgaatataa ctatattgaa gtgtttgact ttatggtgga taatcttgta gaacacatac
     7621 ttgaataaca atttaaaata atttttctaa gtattttttt atactaaata ggattaatat
     7681 gattgaatta atgcaaaaaa taaacaatat aatttataaa ataaatgata aactaaatat
     7741 cacacaaaac ttatagaccc tagagcaaac ccattccact ctactctaag ccatctataa
     7801 tagtttgtat atttctaata atattagata ttaaagtaag aaaaaaattc cttataataa
     7861 ttgctattat atcaattatt gtaaatgttt taataatctt agataggtag gtacatatat
     7921 ggtaaaatcc tcgaacactt ttaataaaaa cttcaacttg atgatattag gtcaaataat
     7981 ctcattgttt ggtgcatcaa tacttaaatt tgctttatct ttatacatac ttgatataac
     8041 tggtaaagca gaaatatttg ctactatact agcagtatct tcaatcccaa ttatagtatt
     8101 ctcccctatt ggtggagcta ttgcagatat attcaacaga agaaacttaa tggttatttt
     8161 tgatttttca agtagtctta cagtactaat cttagcagta ttcttattca acaataatgg
     8221 ttcaatatta cttgtaggaa ttattatgac catattatca gtcataagca caatgtatca
     8281 accaactgtt cagtcaagta cacccctttt agttgataat gagcatttaa tgaatgcaaa
     8341 tggtattgta gctggagttg catctcttac aaatattgca ggacctgtat taggaggtgt
     8401 cttgtatggt gtgattggaa ttaatgcaat tatagttata agttgtgtaa gtttctttct
     8461 atctgcaatt atggagatat ttatacagat tccttttatc aaacaagagc aaaacagcaa
     8521 cattataaaa actatattta ctgatataaa ggatggtatt gttcatatat gtaaagaaaa
     8581 tagatttata ctaaaaatac tttttttagc tatagcaata aacttatttt taagctctat
     8641 gttaatagtt ggtatcccct atatagtaaa aatcactatg ggagctagta acttcttata
     8701 tggtctagca gaaggagcaa tttcttttag ttcactggta gctgctgtta gtattggtat
     8761 tttctcaaaa catctaaaaa tcactaattt gcatatttcc tttattatat gtgcagttat
     8821 gttcatacca atggcggttt cagtatatcc atttatatta aacacaggat ttccagttcc
     8881 atttttaatc tttatagtat catctatgct aatatttttt acaacgactc ttatctcaat
     8941 atatgttata acagtcacac aattaaaaac tccaaatgaa ctcttaggaa aagtaatgtc
     9001 aattcttttt gcaggaacag caattgcaat gccccttggt caggttatgt atggtaaact
     9061 ttttgattta ttatcagata gagtgtacat cattatacta ggagttggat ttattacatt
     9121 acttatagca tttgtatcaa agataacatt gaggaacgaa aatctttaga tacaaagaat
     9181 ttagatataa agatttaatt ttcactaatt tctgaattta aatgcaaaat tgtgtatcag
     9241 tatgttgacg ttttagaaat tgattgatat aatcaatgga aaataaactt tataaaaatt
     9301 caaaatattc agctagagga gtgataaaaa tgagttacat gccaacttta gaacaagcat
     9361 gggaaatatt aagaaaatac aataaggaag aatttcatat aagacatgct caaattgtat
     9421 ctggagttat gagatactat gcaaaggagt acgaccctga aagagtagaa ttttgggaaa
     9481 tagttggact tttacatgat ttagattttg aaatgtatcc tgatgaacat tgtgttaaac
     9541 aaagagaatt aatgacagag cttgatttag ataaatcaat aatcgattct acaataagtc
     9601 atggatatgg attaactggt tcagatgtta aaccagagca atttatggag aaagtattat
     9661 ttgctgtgga tgaacttact ggtttaattg gtgctgctgc aataatgcgt ccttcaaaga
     9721 gcgtttctga tttagaatta aaatctgtta agaagaaatt taaagataaa tcatttgctg
     9781 ctggctgttc tcgagacgtt ataagaagtg gtgcagaaat gcttggatgg gaacttgata
     9841 agcttatcca aacaactata gaagctatga gaagcttgat tccagagatg gcaatatagc
     9901 tcctccatac atataccata gaagttacat ctttaaagag catttctctt tttgatgcaa
     9961 cttcttttta tattttgtag aataaaaagt ttaatatttt aaactcacta tataataaat
    10021 aatataatgt ataatagtca taaactttct aaccttaaaa gtttctaata atcaataaac
    10081 aattct
//
LOCUS       DAYATN010000135        10058 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.135, whole genome shotgun sequence.
ACCESSION   DAYATN010000135 DAYATN010000000
VERSION     DAYATN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.135"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            750..1658
                     /gene="trxB"
                     /locus_tag="ACGI8A_003081"
     CDS             750..1658
                     /gene="trxB"
                     /locus_tag="ACGI8A_003081"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFH0181063.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCITSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1748..3571)
                     /gene="typA"
                     /locus_tag="ACGI8A_003082"
     CDS             complement(1748..3571)
                     /gene="typA"
                     /locus_tag="ACGI8A_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFH0181064.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3783..4025
                     /locus_tag="ACGI8A_003083"
     CDS             3783..4025
                     /locus_tag="ACGI8A_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181065.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            4173..4364
                     /locus_tag="ACGI8A_003084"
     CDS             4173..4364
                     /locus_tag="ACGI8A_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HFH0181066.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4648..7149
                     /locus_tag="ACGI8A_003085"
     CDS             4648..7149
                     /locus_tag="ACGI8A_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFH0181067.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7309..7977
                     /locus_tag="ACGI8A_003086"
     CDS             7309..7977
                     /locus_tag="ACGI8A_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0181068.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7961..9238
                     /locus_tag="ACGI8A_003087"
     CDS             7961..9238
                     /locus_tag="ACGI8A_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0181069.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            9639..9860
                     /locus_tag="ACGI8A_003088"
     CDS             9639..9860
                     /locus_tag="ACGI8A_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181070.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         4021 a         1122 c         1680 g         3235 t
ORIGIN      
        1 attaatataa aaatttggaa aaaatatttt ttaaattgta aaaaaattgt aatcaaattg
       61 agtattagaa aaatattttt ttaacttttt taaaagttta aaaacctaga ttttcatagt
      121 gatgaagctg tttttaatga aagtaattaa aagataacta ttaaatatct gtaatattac
      181 aattttatac tacactatat tacaaaaaaa gtttacaaaa ctttaatttg aaaaatgtaa
      241 ttatatggta tatcatataa atataagtaa atagtgctaa tatttaaact attaatcata
      301 attggttcaa caaaagtata tagttaataa tgtttaattt gaagaaattg ttagtgtgaa
      361 attacatata tattcaaatt aaattcataa atatttcaca taataatagt atactgtaaa
      421 caaaaggagg tattaaatat gggaatagta cttataacag cagcattagt tttggttatg
      481 tatggacttt caattctatc atatatatca gaaagcaata tattagaaga cactgtaaat
      541 gataaccaat aaggttttga attatataaa taagtgagaa ctgtaaataa aagttaattt
      601 ttatttgcag tttttttata tatatgtaaa ataaagttaa ttaataaaaa ataatgtttt
      661 gtttatagta tatatggata ataatagtaa agtataagaa tgatgagaat aatatataag
      721 aaataataat ataaagagag gtaaacgtta tggtagatat cattgtaatt ggtgctggac
      781 cagcaggttt aacttcagca atttatgcta tgagagcagg gttaagcgtt acagtatttg
      841 aaaaaaatat ttatggaggt caagttgcaa gtacctctga ggtagaaaat tatccagcag
      901 ttcaaaagat ttctggggta gaattttcta ataatatata caatcaagca gttgctcaag
      961 gtgttgatat tcagtttgat gaggttgaag agataaattt agagggaaaa gtaaaggttg
     1021 taaaaacttc ttcaggtgaa cataaagcaa aggctgttat tcttgcaaat ggagttgaaa
     1081 gacgtaaatt aggttgtgca ggggaacaag aatttacagg tagaggagta tcttactgtg
     1141 caacatgtga tggagccttt tttaaagata aagaagttgc tatagtaggt ggaggaaata
     1201 ctgcactaga agatgcttta tttttagcta ataattgtac aaaagtgtat ttaattcaca
     1261 gaagagatag ttttagaggt gaagaagtgt tggagaaatc tgtgaaagca agagagaata
     1321 ttgagatact ttacagtcat ggtgtagaaa aaatagaagg agaaaagact gtatcgaaaa
     1381 tagaagtaaa aaacttaaag actgaagaaa aaagaactat agatgttagt ggtattttta
     1441 ttgctatagg tcttaaacct aataataaaa tgtttgaaaa tgttcttgat ttggatgaag
     1501 gtggatatat aatttcagat gagtcttgta taacatcagt agaaggtgta tatgtagcag
     1561 gagatagtag aactaagttt ttacgtcaaa taataacagc agcatcagat ggagctattg
     1621 cagccgttca agcagctaat tatataaatg ttgaataata taaatttcaa ataaaaatag
     1681 ccatataaac tgaaataatt tttcagtact atatggctat tttgttaatt agaggtaaat
     1741 gtacgtttta tttaccttgt ctagatttgt tatatcttaa tctatcatgt tcatttaaga
     1801 atctttttct aagtcttata gaatcaggtg taatttcaac taattcatca tcttctataa
     1861 actctagtgc ttcttcaaga gtaaatattc taggaggaga taattttata gcatcatctg
     1921 aaccagaagc acgtacgtta ctcattttct tgtttttaca agggttgact accatatcat
     1981 cttttctgga attcatacca attatcatac cttcgtaaac ttctacacca gggtctacaa
     2041 acattacagc tctatcactt aaggcattaa gagaataccc catagtaaca ccaggtcctt
     2101 gtgctattaa aacaccattt cctcttgaag gtatttctcc tttaaattct tcaaattttt
     2161 caaatgaacg aactaaagta ccttctccac gagtagcgtt tataaattca cttcgatatc
     2221 ctaaaagacc acgagtaggt gctaagaact ctatttttac atagtctcct tctattgaca
     2281 tagactccat catacctttt ctcatgttta attcatttat aatagtacca gagtaaactt
     2341 caggacagtt tacaacaact ctttctattg gttccattaa ttttccatct tctttatgca
     2401 ttaaaacttc aggtttagaa acaccaactt cataaccttc acgtctcata ttttcaagta
     2461 gtatagataa gtgaagctct ccacgtccag aaactttgta tccatcagta gtatcaagag
     2521 gttcaacttt aagaccaaca tttacttcaa gttccttttc aagtctatct ttcaaatgtc
     2581 tagttgtaac gaattttcca ctttttccaa cgaatggaga gtcgtttact aagaagttca
     2641 tagaaagagt aggctcttct atttttatca tttccatagg tagtggacta tctaaatcgc
     2701 atatagtttc acctatagat atatcaggaa taccagctat aacaactatt tctccactag
     2761 tagcttcatc aacttcaact tgttttaaac cttcatacac agaaagtttt gatacttttc
     2821 ctttagatac aacagaatct tctctacata tagcaacttg tgtattgttt ttaagagtac
     2881 ctttgtaaat tctacctata ccaagtctac ctacataatc atcatatgca agagctgata
     2941 tttggaactg tagaggttca ttatcgtaat taggataagc ttcaacgtga tttacaatag
     3001 ttttaaatag aggagaaagg tcttcactat catcatccat ttcaagttta gcaatacctt
     3061 gtttagcaat accatatatt ataggaaatt cgcattgctc atctgttgca tttaagtcta
     3121 caaataaatc gaaaacttca ttaactactt cttccgctct ctggtctttt ttatcaatct
     3181 tgtttataaa taatattggt tttaaaccga actctaaaga tttttgtaaa acgaatctag
     3241 tttgaggcat tgggccttca ctagcatcaa ctaatagaat aactgtgtca acagttttca
     3301 taacacgttc aacttcagaa gaaaagtcac tgtgtcctgg agtatcaact atgtttattt
     3361 tgtaatcttc atagtttatt gcacagtttt ttgaatatat agttatgcct ctttcttttt
     3421 caaggtcgtt actgtccata acgcagtctt taactacttc gttctttcta aaaacaccgc
     3481 tttgagaaag gaaagcatca accaatgttg attttccagc gtcaacgtgg gctataactg
     3541 cgatatttat aattttatgt ttttgtgaca tttttgttgc tcccttcgat taaaataact
     3601 tattaattat agcacaatat attattaggt acaaggagga atatattaaa ttgtattaat
     3661 atagtttaaa ttgaataaaa ctgttagttt tgcatctgaa aattattttg tttaaaaaaa
     3721 ttttttagac aaataataac cttaatagat aaaaagatta ataaaagagt aaaggatgat
     3781 atatgttgga agtaaccaaa ataataatta aatattttgc aagtgaaagt gatacttttg
     3841 taaaagaaga agtttgtaat tttgaagaac ttcttaagtg gtctcataaa tttattgaag
     3901 gtaaaaagat agaagatgtt ttgtatataa agtctaaaga aaactataaa gtaaatgtga
     3961 ttaagtcttt agaaattaag gaattggaaa tagtgagaaa acaacagata ccaccgtaca
     4021 tgtagtatta ataatatata aaattaatat gtaatactcg caataggata taaatggtat
     4081 atatggtaat aatttatatt aaaaaaactt ttataaagtt tatatttggg tataaaagta
     4141 ttaattagac attaattagg aggaacttaa atatgataag agtatgtcca aactgttcaa
     4201 atgtggatgt aaataagcta aaaactttgg ttggagaaga aaatgtaaag actggttgta
     4261 taggtcagtg tagagcattt aaaaaagaag ctgttggaat aatagacaat gagcttgaaa
     4321 taaaggaaac agaagcagag ttttttgaag catgtaaaga ataaatttta aataaaatat
     4381 aataaataga gcatctaaat tgtataagca ttaaacaatt tagatgctct atttttatat
     4441 atacatttaa ttatgagtat acttttctat taaatacaaa taaatataac taatacattt
     4501 atttattaaa aaaatattga tttaatatac ctataggggg tataataaaa atatagattt
     4561 ctaaataagt aatttatcaa tattacatat caaaataaaa cgttacaaat tagctgaaaa
     4621 tatattgaaa gaaaggaaga ttttattatg agttcaaata aagtggtaga aagttataaa
     4681 attacaggta tgacatgtgc agcttgtgca aaggcagtag agagagtaac taagaagatg
     4741 gatggagtat atgaccaaag tgtaaatata gctactgaaa aacttaaaat agaatatgat
     4801 aattctaagg ttaattttga tgatataaag caggttgtag aaaaagcagg atatggaatt
     4861 ataaaggaag aaagtaataa aaaaattgat atgaaaattg atggaatgac atgtgcagct
     4921 tgtgcaaaag ctgtagagag agtggtaaaa aaattggatg gagtagaaag tattagtgtg
     4981 aatattgcaa cagataaagc taatatagac tatgacccat caaaagtaaa attatctcaa
     5041 ataaaagcag ctattgaaaa agctggatat aaaccaatag aagaggtaaa aaataaggtt
     5101 gatgtggatg aagataaatt gagaaaagaa agagaaatga agtctttatt tgttaaattt
     5161 atagttgcaa tagtatttgc agtacctctg ttttatatag caatgggacc tatgataata
     5221 aagcctatag ggccatggcc tttgccagaa attataaatc ctatgacaaa tacttttaat
     5281 tatgcattaa ttcagttaat tttagtaata ccagtaatga tagctggtta taaattttat
     5341 ataaatgggt ttaaatcact tttttcttta agccctaata tggattcttt agttgcaata
     5401 ggaacattag ccgcattttt atatagtcta tatacgacac tacaaatagc caatggacaa
     5461 attcaaggta tgcaccatca tcagttgtat tatgaaagtg caggaataat aatagcactt
     5521 atcttacttg gtaaatattt agaatcaaaa tcaaaaggaa aaacatcaga agcaattaaa
     5581 aagcttatgg ggctacaacc taaaactgca atagtgttag ttgatggtaa agaagttgaa
     5641 actcctatag aagaagtaga gataggagat atactattag taaaaccagg aactaaaata
     5701 cctgtggatg gagttgtaat agaaggatat acatcagttg atgaatcaat gcttacaggt
     5761 gaaagtatac cagtagaaaa gaatgttgga agtaaggtga caggagctag tattaataaa
     5821 aatggtgtta ttaagtttaa ggctgaaaaa attggtggag atactgctct tgcccaaata
     5881 ataaagttag ttgaagatgc acaaggtaca aaagcaccta ttgctaaact tgccgatact
     5941 gtatctggat attttgtacc aatagtaatt gcaatagctg tagttgcatc attattgtgg
     6001 tttttaatag gaggaaaaga tatagtattt gtacttacta tatttatatc agtacttgta
     6061 attgcttgtc cttgtgcatt aggattagca acaccaactg ctataatggt tgggactgga
     6121 aagggcgcag aaaatggaat cttgataaaa ggtggagagg cattagaatc tgcacataaa
     6181 gtaaatacag tgatatttga taaaacaggt actataacag aaggaaagcc aaaagtaaca
     6241 gatatagtat taaataataa tgtaaaagaa gaatatctta tcaagattgc ttcaagtgca
     6301 gaaaaaggtt cagaacatcc tcttggagaa gcaatagtta agtatggaga agagaagaat
     6361 ataaaatttg aaaaagttga taattttaaa gccataccag gtgctggtat acaagttact
     6421 attaatgatg aaagtatatt acttggaaat agaaagctta tgaatgacaa taatataaag
     6481 cttggggatt tagaagaaaa atcaaatata ttggcaagcc aaggtaaaac acctatgtat
     6541 atagctgtag atggaaattt atcaggtata atagcagttg cagatgtagt caaagaaagt
     6601 agtaaaaaag caatagaaat acttcatgat atgggaataa aagttgcaat ggtaactgga
     6661 gataatgcga aaactgcaaa tgctatagca aatcaagtcg gtatagatat ggttttggca
     6721 gaggttttac cagaagataa gtcaaaagaa gttgaaaaac tacaaaatca aggaaagttt
     6781 gttgcaatgg taggggatgg aataaatgat gccccagctc ttgcaaaagc agatataggg
     6841 atagcaatag gaagtggaac ggatgtagcc attgaatctg cggatatagt acttatgaag
     6901 agtgatttaa ttgatgttcc aacagcaatt aaacttagtc atgaaactat aaaaaatata
     6961 aaacaaaacc tattttgggc atttggatat aatacaattg gtatacctgt agcagcagga
     7021 atattatatg tatttggagg acctttatta aacccaatga ttgcagcagc agcaatgagc
     7081 ttaagttcag tttcagtcgt atcaaatgca cttagactta aaaacttcaa ggcatataag
     7141 agagattaga ttaataaatt aaataaaatg tatccaaaag agctatcttt aaattaaaag
     7201 gattaattta caaattaaga tagctctttt ttcatgtaaa attcacaaca ataagataaa
     7261 atataagtaa tatacgaaat tttaaataaa atagaaagga ttgatagaat gaattcgtca
     7321 atacttgtaa ttgaagatga ttcaaatata caagagttaa tatcagaatt tttaagtgca
     7381 gaaggttatc aagttgatac agcaaatgat gggttggaag gtatacaaaa gtttaagcaa
     7441 ggaagttatg atttagtaat attagatata atgatgccaa acttagatgg ttatggagtt
     7501 tgtaaaatga taagaaagtc atcaagcgtt ccaattatat ttttaactgc tttaaatgat
     7561 gaaggagacc aacttaaagg gtttgactta gaatgtgatg attatataac aaaaccattt
     7621 tcatttaatc ttcttataaa aagagtagaa gcaattttaa ggcgaagtaa caagactata
     7681 aatgacaaat ttatagtttt tgaaaaatta aaattagatt taaacacata tattgcagag
     7741 atagatggag aacctataga acttacacta aaagagttta atatattaaa agctttgata
     7801 gaaaagtatc cacaagttat aactagggaa ggtcttttgg atagtatatg gggttatgac
     7861 tattatggag atactaggat tgttgatgca catataaaaa atataagaaa aaaaatatca
     7921 ttaccatata ttaaaactgt aaaaggaata ggatatacac ttgagaagga tatttgataa
     7981 gtgggaaaag ttaagtataa agtataagtt attttcaata acgacatcat tattaatagc
     8041 attagcttta ataatatatc taattttata ttttttattg ccatcatatt atcatgagta
     8101 taaaattgag tctcttcaag aaagtttgaa atctttagta gatagttcaa ttcatttcga
     8161 tacatatact ttagaagaaa gactttatta tatggctaaa gaccaaaatt tggctatttt
     8221 attaaaagat aaccaaggta aaattgtata tggtaagaat gaagtagtta ttttaagata
     8281 tagtaagtac atgattaata gtcttgagga tgagtataga acttctatac ctatatatac
     8341 taaagatgca aaagatggtc catatacttt agaactcgtt atgccattac aaccaattga
     8401 cgaagcaaac gaagttataa gaaaactaat gccatacatc atatcaatag caatattgat
     8461 agcgattatt ggagcatata tatattcaat tgtaataaca aagcctctta taaacataat
     8521 agagagtgag agagaacagg aatatagaag gaaggatttt gttgctacaa tatcacatga
     8581 actaaaaact ccaataacca taataagtgg tcaaattgaa ggtatgattt atagtgtagg
     8641 taagtataag gatagggata cttatctgaa aaaatcgtat gagtgtacac aagaattaaa
     8701 agatttagta aatgaaatga tagaggtttc aaaatcagaa atacttgaaa aagatttgaa
     8761 attggtatca ataaatataa gtgaattatt aaatagactt gtaaagagac aagtattttt
     8821 aatagaagaa aaacatatga aaactatttt aaaaatagaa gaaaatttgg aaattaaagc
     8881 agaccaagag agaataacta aggcaatcaa taatataata aataatgcca taaaatattc
     8941 tcctgaagaa tcagagctta taattagact ctatgataaa aataaacgaa taagtaaaaa
     9001 gacttctaat caaagagtgg ttttagaaat tgaaaatact ggtgtaacta tcgagaaacg
     9061 ttatttagaa gaaatattta atccatttta tagaatagaa aaatctcgtt cgagaaaaac
     9121 tggaggaagt ggacttggac tttatatagt cagccaaatt tttaaaagtc atgggtttga
     9181 ttatagcata aaaaacaaag aaaactctgt agtattcaca gtagaattta aaaattaatt
     9241 acttattata aagaatatat aactttcaat aatagttcat tttaaaatca caaaaaattc
     9301 ataatgaaac tgtattatat aaatataaat aagcttaaaa ccataaaata caccaactat
     9361 aatgatgaga tttccttaca tcctccatat aagtcatcat tatagtaacc caacaattaa
     9421 gttactatga atttaaaaaa taacaccgag catataatct atgcttagtg ttatttttat
     9481 tttgaaataa aaaatattta atttattgaa atgtatagat ttagatagtt taaactatat
     9541 ttaagactta aaaattataa ttgatattac aaataaatat atgtactttg ggaaaaataa
     9601 atataggaag tattatattt acagaggagg tagttttgat ggatagatta aaaagagata
     9661 tgaaaaatat gaaagaagat ttaaaagaag gtgttaaaaa gacagcttat gttatgaaaa
     9721 atacagctga agatttgaaa gatgatgcac aaggtgctat ggtaaactta gaaatgaaaa
     9781 aagatgagat gatggaaaga tatgaacaaa agaaattttc taaagaatta gagaagcaaa
     9841 tgaagaatga aatgaaataa gcttttattt atgaataaca aattattgta ttcatataaa
     9901 aagtagccag taatattaaa tagtttattt aaggaaatag actttctaaa aataactgta
     9961 ttagatttag aaagtctatt tcaaattagt tttataaata gacataagga taaagtagat
    10021 gttatagaac tagattttaa aaaatagata ctaaggat
//
LOCUS       DAYATN010000136         9797 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.136, whole genome shotgun sequence.
ACCESSION   DAYATN010000136 DAYATN010000000
VERSION     DAYATN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.136"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            383..1312
                     /gene="glsA"
                     /locus_tag="ACGI8A_003089"
     CDS             383..1312
                     /gene="glsA"
                     /locus_tag="ACGI8A_003089"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFH0181071.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1410..1973
                     /locus_tag="ACGI8A_003090"
     CDS             1410..1973
                     /locus_tag="ACGI8A_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0181072.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2461..3297
                     /locus_tag="ACGI8A_003091"
     CDS             2461..3297
                     /locus_tag="ACGI8A_003091"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008833 - deoxyribonuclease IV
                     (phage-T4-induced) activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFH0181073.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3581..4576
                     /locus_tag="ACGI8A_003092"
     CDS             3581..4576
                     /locus_tag="ACGI8A_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFH0181074.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4833..5237)
                     /locus_tag="ACGI8A_003093"
     CDS             complement(4833..5237)
                     /locus_tag="ACGI8A_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFH0181075.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            6259..7173
                     /gene="queG"
                     /locus_tag="ACGI8A_003094"
     CDS             6259..7173
                     /gene="queG"
                     /locus_tag="ACGI8A_003094"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFH0181076.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNISNKEFKR
                     KYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7279..9516
                     /locus_tag="ACGI8A_003095"
     CDS             7279..9516
                     /locus_tag="ACGI8A_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0181077.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
BASE COUNT         3993 a          865 c         1809 g         3130 t
ORIGIN      
        1 ttgttgaatt actaaattac taggttgtta agttactgga ttattagata gttagattac
       61 taggttgtta aattgttaaa ttattgaatt gttagattgt tgaattacta aattactagg
      121 ttgttaagtt actggattat tagatagtta gattattaga ttgctaaatt attaggttgt
      181 taaattactg gattattaga ttactaaatt gctaggttgt taaattatta aattattaag
      241 ctatttaaac tatttaaatt gtttgaaaaa tgaattgatt cttgtagaaa ataattttaa
      301 agtataattt tttttaacat tgttttatta tagtataaac ttgtgctaaa atagaatata
      361 taacataata taagaggtgc atatgaatac gttagatgag acattattaa aagaaattat
      421 aagttctaat aagaagtata ctaattatgg acaagtcgct agttacatac cagaattaaa
      481 aaacgctaga agaaatgatt taggaatttg tatcatagat agcgaaaata atctttatag
      541 tgctggaaac tgtagcacaa agtttactat acaaagtatt tcaaaaccaa ttgtgcttgc
      601 tatggcactt atggataatg attgggaaga cgttttttca aacgttggaa tggagccaag
      661 tggggaccca tttaattcaa taatgaaact tgaaattaat gacacaaaaa aaccctgtaa
      721 tccaatgata aatgcagggg ctatagtaac cacttccctt ataaatggga gttgtttaga
      781 agaaaaagaa gaaagaatgc ttagtttctt tagaaagtta gcaaaaaatg ataatatagg
      841 aataaactat gatgtctata agtctgaaaa aatgactgga gatagaaaca gagctatggc
      901 ttacttattg aaaagtgatg gatttatcag agggaatgta gaagatgttc tagaccttta
      961 ttttaaacaa tgctcaattg agatagattc tgtagattta gcgagaattg gaataaatct
     1021 tgcaaattat ggagtagata tagaaaatgg tgagcattta atgagtgaaa tggtttctag
     1081 gattgtcaag acatttatga tgacttgtgg aatgtatgat gcttctgggg aatttgctat
     1141 taaagttggc attcctgcaa aatctggagt tgggggtggt ataatggctt ctgtgccggg
     1201 acgcatgggg attggtgttt atggacctgc gcttgacaaa aaaggtaaca gcgtcgcagg
     1261 agttaaagtt ttagaggagt tgtcaaataa actcaaatta aatatttttt aaaatattaa
     1321 gaaaggattt ttttgacaac aacatataat ttaatatata attaaattgt aaagaaatat
     1381 gaattaaata aattttttag gaggggtcaa tgaaagaggt attggtatta cgagatttgg
     1441 agtgcataaa ggctattgcg catccacgta gaatagatat tctaaaagca tttgacaaat
     1501 cgccattgtc agcaaaacaa ttatctcaat tattggaaga acctcatgca aaaataaact
     1561 accatataaa aatgttatac aaagttggta ttttagaatt agttgaggaa aaaataaagt
     1621 cggggattgt ggaaaagtac tattatccaa gtgcaaaaaa tgtggttata gggaatagga
     1681 tacttaattt ttctttagac aatggggaag aaaaagaaga attgtacata tcaaaatttg
     1741 aaaatatgag tgaagttttt tataaggcag cagaggaaga tgttttagaa aatgaaaata
     1801 tagttgatta tcatgatatt tctcttactc atgatgaatt agttgaactt agtgacacta
     1861 tgaagtcaaa gatagatgaa attttaaata aaagacaaca caatgttgaa ggttctaagt
     1921 atgatatagc tatggttata gttcctactc ttgaggagga atgtcctagt tagagaatta
     1981 attatggatt aattaaagat ataatattag agattgaaca tcaaaattta cttaataatc
     2041 aaaaaatagt ttttggtatg ggtaaaacaa tgatttttaa tctctatttt tttgtttttt
     2101 tatacctatt gtatttgtaa ataatatgta tagtgtaaga tgtatacact atctaagaag
     2161 tgcttagata atatacaaag tatgtaagat tataatattt gaaattgatt aaaatgggta
     2221 taattaaagt gtaaatatta aaattaaaaa ttaatataaa ttaaatcaat gctagaaata
     2281 tgttaaaaga ggaatgagat tagttgggat agaacatccc ttagagctta cgaattttta
     2341 gtgaaaaagt actatcaaat aggaatctcc atcttcatat gttggctaaa tgtagttaag
     2401 gtggagtagt tcaaaattaa aattgagtac aagtagtagt acaaataaaa ggagattaat
     2461 atgattaaat taggaacaca tctttcaata gcaaaaggat ttgctaatgc ggctgagaca
     2521 gctgtatata ttggtgcaaa tacatttcaa ttttttagta gaaatccaag aggaggaaat
     2581 gctaaagaat ttgatgaaaa agatataatt aaatttcaag aaattagaaa agaaaataat
     2641 tttgcaccac tgcttgcaca tgcaccatat actatgaatc tgggtggtac aaaggatgat
     2701 gtatacgaat ttgcaacaag ggtgatagat gaggatataa gaaggatgga ctcattagga
     2761 attgaataca tgtgttttca tcctggaagt catgtaggtg gtggtgtaga ttttggcata
     2821 gatagaatag taaaaggatt gaacaatgcc ataaagggtg atgaaaatat aactatactt
     2881 cttgaaacta tgtcgggaaa gggaactgaa ataggtttta aatttgagca attaaaaagt
     2941 ataatagaca aaattgagca taaagaaaaa ataggtgtgt gtcttgatac atgtcatatt
     3001 ttttcagctg gttatgatat agtgaatgat ttagatgggg ttttggaaga atttgataag
     3061 gttataggca tagaaagatt gaaaactgtc catctaaatg atagtatgat gccatttgga
     3121 gacaaaaaag acagacatgc tcctataggg gaaggaaaga taggattaaa agcattaatt
     3181 gattttatgg aacatcctag tctaaaacat ttaccatttt tcttagaaac tccttttgat
     3241 gatgaaggtc acaagagaga gcttaaaatg ataaaagaaa tactatctag taaataaacc
     3301 tttattaaaa atatgtgaaa tactccaccc tcaacataat tagggtggag atacaatcat
     3361 gaaatgtgat aatttaaatt aatggatagg aataacgaaa attgtataca aattaactgg
     3421 gtataaagta tgattatatt atgaatagac tgatataaaa tataattata ttatgaatag
     3481 tatgtaaata cattgcaaag attctcataa tttgttgtat aattaaaggt gacatattta
     3541 tattatataa aaaattatta aaagttaggt ggggaaagat atgagaaatt taggcaatac
     3601 taacatgaaa ataaagagag ttggctttgg gggcattcca atacaaagga ttactcaaga
     3661 tgatactaac ttagttataa atgagttaga aaaacaagga ataaacttta ttgacagtgc
     3721 aagagggtat acaataagtg aggaagcaat cggaattgcc attgaaggaa aaagagataa
     3781 atttttctta gctactaaaa gtatgtccag agattatgat tcaatgaaga gagatgttga
     3841 aataagttta aataatttta aaactgattt tatagattta tatcaattcc ataatgttaa
     3901 agaagaggag tatgataatc tttttaaaga taaaatggct tatagtgccc ttttagaggc
     3961 aaaggagcaa ggtaagataa aacatattgg tataactagt cacaatttaa atactataga
     4021 aaaagctata gaagatggaa aatttgacac tatacaattc ccttataata tagttgaagg
     4081 acaagcagat gaagtgttta aaaaagctca tgaaaaagga attgggataa tagtaatgaa
     4141 acctttagct ggaggtgctt tagacaatgc aacactggct attaaatata tactatctaa
     4201 agagtacata gatgtagtta taccaggtat ggagagtgtt gaacaggtta gacaaaatgt
     4261 cgctgtactt gaaaatcttg tgctagatga aaaagataat aaggaaattg aagaaataag
     4321 aaattcttta ggtaaaaaat tctgtagaag atgcgaatat tgtatgcctt gtgctgtagg
     4381 aataaatata ccattgagtt ttttatgtga aggttattat actagatatg gattaaaaga
     4441 gtgggctaaa gaaaaatatg aggttatgga tgttaaacct actgagtgta ttgactgtgg
     4501 gctttgcgag agtagatgtc catatgaact tcctataaga gaaatgctta agactgttgt
     4561 tgaaaagcta ggatagattt tatttattga aatcggtgta ttaaatttac tccgatttct
     4621 ttttattaga tgtggtctta tatacaaata ttaaatttac atagttatag ctattatata
     4681 aatttaatat ttgtttaata cttattaaat atttacttaa tgctttttaa aaacatttgt
     4741 ttgagtatag acataacaca attattattg ttgtattagt atagtttcaa atactggagt
     4801 atcaatataa tgtcgaataa cacataaaat ctttattttt caaaaatatt ataggatata
     4861 tcgtatttag atactggata aagttcaatt tcaaccctag ggttatcttt atctataaaa
     4921 tcaaataaaa gtactggctt aagttgttta tcatttttta ttattccact tttttcaata
     4981 ccatcacaaa tactttttgg ataattatgt aaatccccca ttctcttatt tgggaagaat
     5041 aatttcaaga ctgttattag attttcttcg acagtttccc cttgatactg ttggtttata
     5101 aaacctgcaa tcatgttttc gtatgcaaga tacttagaat attttccctt tgaaggcatc
     5161 catgcttgac catttatgtt atgaagctta aaattagatt tactaatagg tctacctgga
     5221 attgttattt taatcaaatt tttcaccatc cttatacagt atttattata atctgtatat
     5281 gatttaaaag caaattgtta tttaaagtgt ttttacagag cttttttgta aaattttcca
     5341 ctttagatgt agtaatataa gataagtatt ttgcttacaa attttaaaat ttgatatatg
     5401 atggacatat tcatattagt ctattataag accttcataa tattaataag tatagtttag
     5461 taaagagagt aaaaagaaat ccatataagt caagtaaaga tttaataaat ggcaatgtaa
     5521 atgggatatt aattataaga aaaaatcaat ctttttgctt aactatagaa agaatgtcaa
     5581 aatataacta atgatatttg aagttttaga gtttatatta aaaggtaaga aggttaagtg
     5641 ggtaaaaaag gttgaataga ttaaaaaggc taagtatcta aaagagttaa gtaggtaaaa
     5701 aagttaaata agtaaatgaa aaaggctaag tatctaaaag agttagtagt taaataagta
     5761 aatgaaaaag gctaagtata taaaggagtt agtagataaa aaaattaaat agataaaaag
     5821 attaagtatc taaaagcgtt agtagataaa aaaattaaat aaataaaaaa gactaagtat
     5881 tcaaaagagt tagtaggtaa aataattaaa tagataaaaa gactaattat ttaaaggagt
     5941 taagtagata aaaaagttaa ataagtaaat gaaaaagacg aagtatctaa aagagttagt
     6001 aaataaaaaa gatgaagtat ctaaaagagt tagtaggtaa aaaactaaat agataaaaag
     6061 attaagtatc taaaagcgtt agtaggtaaa aaattaaata ggtaaaaaga cgaagtatct
     6121 aaaagattta agtaggtaag aaaattgaat agctaaaaag attaagtatt taaaagagtt
     6181 agtagctaga aaaggttaaa aagactaaaa aaattaaata aatttaaaaa gtcaattaga
     6241 ttaaaaagga ataattttat ggataataag gaattaaaag cattttgtaa tttaatagga
     6301 ttaaactgtg taggagtagc aggggtagat aagtatgata atttagaaaa gatacttaag
     6361 gacagacaac aaaaaggtta tttaactggt atggaagaac caataattga gaacagaatt
     6421 aacccaagac atataatgaa ggatgctaac tctataatag tctgtgcatt tccatatcat
     6481 ataaatagac aagaaaagaa taaaaattca aatttatcaa aatattgtca tggaaaggat
     6541 tatcacattg tagtaaagga ctttttacaa caaatttgtg attatatttc taaaacagta
     6601 acagatttta aatacaagtt atttgctgat aatggaccat tagttgacag gtatttagct
     6661 tatttatcag gaataggata ttttggaata aataataata tcataactga tgaatatggt
     6721 tcttacgtat ttatagggta tatagtaaat aattataaat ttgaagaaga taaaccacta
     6781 gaaaaaagtt gttttaaatg tggaaagtgt gtaaaatatt gtccgggaaa tgctatattg
     6841 ggaaattatg atatgaaccc caaaaaatgt ttatcatata taactcaaaa aaaaggagat
     6901 ttgtcagaga aagaaaaagt tgttttaaag aatggcaaaa aggtatttgg atgcgatatt
     6961 tgtcaagaag tatgtccaca taatgttgaa ataccaacaa ctcatatttt ggaatttaaa
     7021 gaaaatataa tagattattt gaattatgat gagatacaaa atatttcaaa taaagaattt
     7081 aaaaggaaat atggagatag agcttttagc tggagaggaa gaaatataat aaagagaaat
     7141 atggaaataa ttttagataa gtatgatgac taataataag agtctgtttc atatacttta
     7201 atgtgtattt actttaacac ttgttgtgta aaagtcatat tatatcatta aagttaaata
     7261 tggggaggtg ggggaatatt gaaatataat aatacatctg aaataaatcc actagattat
     7321 atattgggtc aagaaagagc tgtagaagct atggaattag ggttgaaaat aaataatcca
     7381 gcgtacaaca tatatatagc tggtgaacct ggaactggta aaagtactta tgccttaaag
     7441 gttttaaatg agtatgcatc caaaaaaaat acgcataaag attggtgtta tatatataat
     7501 tttgaaaacc ctagagagcc tattatagtt gaattagaaa aggggtttgg aagagaactt
     7561 aagaaagata tggagaaatt aattgaaagt cttttagaag attttaaaaa tgcgtttgaa
     7621 agtgaaaatt ttgaaataga aaaaaataaa cttctggatg aatatgaaat agaaaaagat
     7681 atgcttttga agcaaattaa aaaatatgga gaagaaaaag gatttaaatt aaaacagagt
     7741 aaaatgggaa tagtttttat ccctttgaaa gaagaggaag atgaaagtga tgagtctgat
     7801 gaagagtttt acaaagctaa aagagaacta gaaaatatgg cgatacaggt cgtatataaa
     7861 attagaaact tagaagaaat agctgaaaaa gcagtgttag agcttgaaga agagatagct
     7921 aaacttgtag tagaacctca tatcaagaga ttgtgtgaaa aatatgaaaa ttatgataag
     7981 attcaaactt atctggaaaa tataaagaaa gatataatag aatatatgta tttattctat
     8041 ttagatgaag aggagttaaa agataagtat gataaagagc attttattaa atataaaatt
     8101 aacctatttg tagacaatgg aagctcaaag aataaagaat cagcacctgt tgtagtagag
     8161 ataaacccaa gtccagcaaa tctatttgga aaggcagagt atgattatgc aaatggaaat
     8221 ataaagactg atttcacaaa actattatca ggagcatttc atagggctaa tggtggttat
     8281 ttagtacttt atgcagacca attactaaaa tatacaatgt cttgggaaat tcttaaaaag
     8341 actttgcaga ctaaaaaagt aatattggaa acacaaacag caattaaacc agaaaatatg
     8401 ccattggatg ttaaattagt actgattgga agccactata tatatgatat tttatataga
     8461 tatgatgaag attttgaaaa atattttaaa gtatttgtag actttgatag tgaaatggat
     8521 aaaaatgaag ataatgaaga aggaatagca agatttatag cataccaatg tgataagaat
     8581 aatttaaggc attttactaa atcagcagtt gaagaggtta ttaaatttag cactagatta
     8641 tctggtgatt tagaaaaatt atcaactaag tttaataaaa taatggaagt tgtaatagaa
     8701 ggaagtgctt atgcagagtt tagaaattcg gagtatacag agcagtgtga tgttaaaaaa
     8761 gcaataagtg aaaagagaaa aagaatcaat agagtggaaa cacatatgga tgaatcaatt
     8821 gaaaatggat ttactctaat agaaacagag ggaagaaaag taggagtgat aaatggtttg
     8881 tcagtcttaa gtacaggaga atactctttt ggtagagctt caaggattac agcgaccact
     8941 agcccaggaa gtaaaggcat agtaaatata gaaagagaag taaatatgag tggctctata
     9001 cataacaaag gagtacttat attaggtggc tatctttctg agaattttgc acaggattta
     9061 ctattgtcac taaatgcata tgtttgtttt gaacaaaatt atggtggaat agatggagat
     9121 agtgcatctt ctgcagaatt atatgtacta ttatcatctc ttagtggagt atcaattaaa
     9181 caaaatattg ctgttacagg ttctataaat caaaagggtg atatacaagt agttggtggt
     9241 ataagtgaaa agatagaagg attttattct atatgtaaaa ctaaagggtt aaatggtgag
     9301 caaggcgtca taattcctag aaaaaatcaa agaaatctcg ttttatcaga tgaagttaat
     9361 gatgcagtgc gagatggaaa atttaagata tatactgttg aaagagtaga agaggctata
     9421 gagatattga cagatgttaa atttgaagaa ataaagaaat tggtaaaaga aaaattaata
     9481 tctttcagca agatacaaac tgtgtcaaaa gaataagttt attatagaat ttaatgggta
     9541 tttatgtata ataaaaagca agaactttaa taggtttttg ctttttttgt aaatatatat
     9601 aaattaaatt cttattttgt atgttttata tattgatgtt tatttttttg ttgatatgat
     9661 taaactattt ataaatagcg ttttataaat tggtattttg aagggggata attatgaaaa
     9721 atgtaaatct aaaagagata aatgaaaaag atgtaatttt ggaagaagag ataaataaaa
     9781 aaggaatgga taagaaa
//
LOCUS       DAYATN010000137         9575 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.137, whole genome shotgun sequence.
ACCESSION   DAYATN010000137 DAYATN010000000
VERSION     DAYATN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9575
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.137"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            187..1692
                     /locus_tag="ACGI8A_003096"
     CDS             187..1692
                     /locus_tag="ACGI8A_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFH0181078.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2082..3179
                     /gene="dinB"
                     /locus_tag="ACGI8A_003097"
     CDS             2082..3179
                     /gene="dinB"
                     /locus_tag="ACGI8A_003097"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFH0181079.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            3442..4578
                     /locus_tag="ACGI8A_003098"
     CDS             3442..4578
                     /locus_tag="ACGI8A_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181080.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            4571..6118
                     /locus_tag="ACGI8A_003099"
     CDS             4571..6118
                     /locus_tag="ACGI8A_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0181081.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            6453..7319
                     /locus_tag="ACGI8A_003100"
     CDS             6453..7319
                     /locus_tag="ACGI8A_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFH0181082.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            7460..8602
                     /locus_tag="ACGI8A_003101"
     CDS             7460..8602
                     /locus_tag="ACGI8A_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFH0181083.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            9004..>9575
                     /locus_tag="ACGI8A_003102"
     CDS             9004..>9575
                     /locus_tag="ACGI8A_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181084.1"
                     /translation="MSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDLNNTYDV
                     LLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTDFNSAYG
                     FGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNVERFIYS
                     QFSLIFFIVIANKMKGKNSYLVELAAIPLFG"
BASE COUNT         3679 a         1013 c         1666 g         3217 t
ORIGIN      
        1 cttattttat tagttatata ggtatatctt atttaccata aaactatctt gaaaaaaatt
       61 aatcttaatt gtataatatt actttgagtg aaaaagtatt aacatttttt tataagatgt
      121 tttactaaaa aaatatatat aaatatactg ttatcaacaa gaaatttaat ttagtgaggt
      181 gttaagatga aagtaggatt tgaccacaaa aaatatttag aagagcagtc taaatatatc
      241 ttagaaagag taaataattt tgacaaactt tatttagagt ttggaggaaa attgatggga
      301 gaccttcatg caaaaagagt acttccagga tttgatgaaa atgcaaagat aaaagtatta
      361 caacatatga aagaaaaagt tgaagttgta atttgtgtct atgcaggaga tatagaaaga
      421 aacaaaataa gaggagactt tggtatcact tatgatatgg aagttttaag attaatagat
      481 gatttaagag gttatgaact agatgtaaat agtgtagtaa ttactagatt tgaaggacaa
      541 cctgctacaa ctgtatttat aaataaatta gaacgtagag gaattaaagt ttataagcat
      601 tatccaacaa agggatatcc gtcagatgtt gagactatag ttagtgatga gggatatgga
      661 gcaaatgaat atatagaaac tacaaagcca atagtagtag tcacagcacc aggacctaat
      721 agtggaaaac ttggtacttg tctaagtcag ttgtatcatg aaaataaaag aggtaatgta
      781 gttggatatt ctaagtttga gacattccca gtttggaatg tacctgtaaa acatcctttg
      841 aatatagctt atgaagcagc tacagttgat ttaaaagatg tgaatatgat agattcattc
      901 caccttgaaa cgtatggtga aatgtcagta aactacaata gagatttgga attatttcca
      961 gtgcttaaaa aaataataga aaagataact ggaaaagaat caattttcca atctccaact
     1021 gatatgggtg ttaatagagt tggctttgga ataatagatg atgaagtaat aagaaaagct
     1081 tctatagaag aaataataag aagatacttt aaaactgcct gtgagtataa aaagggacaa
     1141 gttgataaag gtgcttatga tagaatgaaa atgattatgg aagagcttaa cttaaagcca
     1201 gaagatagaa atgttgtatt acctgctaga aactacagta ataaattgaa agaaagtgcc
     1261 gacaagaatg atacttgtcc tgtagttgca ttagaattag aagatggtac aatattaact
     1321 ggtaagggtt ctgatttaat gaatggaact gcagcagtag tattaaatgc cattaaacat
     1381 ttagccaata tatctgatga tatgcactta atatcacctg taatattaga acctatcata
     1441 aacttaaaaa caaagacttt agctagtaga aatgtttcac ttagttgcca agaggtgtta
     1501 actgcactta gtatttgtgc tgtaactaat ccaactgcac aatttgctat ggaaaagtta
     1561 gcattattaa aagggtgtca agctcattct actactatat taaatagaga tgatgaacaa
     1621 acatttagaa aacttggtat agatgtaact tgtgacccag aatatccttc acaaaatctt
     1681 tactatagtt aaaattatat aaaaaaatat gggctgtttt aaagtaatag tttgagcagt
     1741 ccattacaat tataataaaa tcagtaggct gtcttgtata agacagtctt ttgtttttaa
     1801 aacatcccat aaattaaata tatatactgt tatccataag gaattttaaa tttagttcct
     1861 tggaatacct attgtaaagc ttttatatag tttttgcaag tgtttttaag gattttttat
     1921 aaattatatt gaagttaatg gattatagat ggttttattt atggaaaata tgaatatata
     1981 aagaaaataa tgaaaaaata ccaaaaagaa acataagttt ggtataatta aaaagtaata
     2041 taaataaaat taatctattt atactttaaa aggtgataat aatgaaacat tatagaaaaa
     2101 taattcatat agatatggac gctttttatg catctataga acaaagagat aataaaaaat
     2161 taaaaggaag acctgttatt gtcggtggaa atccacaaag ccgaggtgtg gttgcaacct
     2221 gttcttatga agctagaaaa tttggtatcc attcagctat gccatcagct gtagcttaca
     2281 ataggtgtcc atatgcagtt tttgtaagac ctagatttga tgtttataag agtgtgtcag
     2341 agcaaataag agatatattt tatagataca cagatttagt tgaaccatta tctttagatg
     2401 aagcttactt agatgtaaca aaaaataaaa aaaatattga ttcatcaatt gagatagcaa
     2461 agcaaataaa aaaagatata tttagagaag ttggtcttac ttcttcagct ggtgtgtcat
     2521 acaataagtt tcttgctaag attgcatcag acttaagaaa gccaaatggg ttaactgtaa
     2581 taaccgaaga aaatgcccag gactttttag ataaattacc tgtgaataaa ttttttggtg
     2641 ttggaaaagt tacttcaaat actcttaaaa atttaggtat aaaaactgga tatgatttaa
     2701 ggtgtttaaa tttatttgaa cttgaaaata tattcaaaaa aagaggttat gaattatata
     2761 aatttgctag aggaatagac gatagaccag tggaaccaaa tagggttaga aaatctgttg
     2821 gggctgagac tacattaagt cataatttgg atattgatga agaagaaaca cgcaacattc
     2881 ttgatgaact ttgtgaagaa gtatgtcata gattaaaaaa ctctgagaag tttggaaaaa
     2941 cattaacatt aaaaattaaa tatgaggact ttacaaagat aactagaagc cttagtttag
     3001 aacactatat tgacgaatat aatgatataa gaagtggtgt agataatttg ttgagaaatg
     3061 ttgaggttaa tggaaaacaa attaggctat tgggagttac aatatcaaac ctaagtgata
     3121 aaaaagaaac ttataaagat attacattat ttgaatacat ggatagtatc caaatgtgag
     3181 ttttgtttaa atgaaataac actagcttta atactatata attgaggcta gtgttatttt
     3241 atgaatattt ttatattttt tgttttttga cattgtatat tatatactat atataaatac
     3301 tttatatttg tagcaatatt cagacaaata gtgacctaaa aatcgtactt tgtttacata
     3361 aataaaatag tatgatataa ttataaaaac aacttataat attggagaag gttgcttgtt
     3421 cgaaaaaggg gatgataaat tatgatagag ataagaaatg taacaaaaaa aataggaaat
     3481 aatgttatat tagatgatat ttctctagtt gtagaaacag gtacattggt agttttgatt
     3541 ggttcaagtg gttgtggaaa gactactact ttgaagctta taaacaaatt aataaaacct
     3601 acatctggtg agatatatat taatggcaaa ccaatttctc aggaaaatga aatagagctt
     3661 agaagaaaaa taggatatgt tatacagaac acaggcctat tccctcatct tacaataaaa
     3721 gaaaatatag agctaattcc acgcctaaaa aaggaaaaat ccgttgaaga aattgaaaaa
     3781 agaacgttgc aactacttga gatggtagga cttgatagtg atgaattttt gaataagtat
     3841 ccttcagaac ttagtggtgg acagcaacaa agaatcggtg ttgcaagagc cattgctact
     3901 gatgctgaaa taatattgat ggatgagcct tttagtgctt tagacccaat aacgagaaca
     3961 tctttacaag aacaattatt ttctcttcaa gatgaattga aaaaaactat aatatttgta
     4021 acgcatgata tggatgaggc tttaaagata gctgataaaa tttgtattat gaaggatgga
     4081 agaatagcac aatatgatac tcccgaaaat atacttagaa agcctgcaaa tgactttgta
     4141 aaagatttta tcggagaaga tagagtttgg gataatccag aatatataaa agcaagagat
     4201 attatgataa aaaatcctat tgctgttaat tctactagaa ctgtaactca aggaatagaa
     4261 attatgagaa cgagtaaggt tgatagctta ctaattatag atagagcaaa aacattaaaa
     4321 ggtattgtta ctgtaaaaga tatgaaagat attgatgata agtcaatatt acttgctgat
     4381 attatgtcaa gtgagccttt gcatgtaaat gaaggggata atttagtaga aatactaaat
     4441 gtaatgaatc gtaattcggt tggatatatt ccggttattt ctgatgaaaa taaactggta
     4501 gggcttatta ctagaagtag tttactttct gttttaagtg aacaattctt agaaatggag
     4561 gtgagtgttc ttggctaatt tttttaactt tatactatta caaaaagata agattataga
     4621 gcttttaata caacatatga gtctaactgt tacatctatt ttaatagcta taatagttgg
     4681 tgttcctctt ggaataatta tttcaagaat ctcatctctt agaaagtttg tacttggatt
     4741 tgtaaactta gtccaagcag ttccgtctat ggcacttttg ggtcttcttg tacctatact
     4801 tggtatagga agtaaaccag ccatatttat ggtagtggtt tattcattac taccaattgt
     4861 aaaaaatacg tatataggta taacaagtat tgacccagta gtattagaat cagctaaagg
     4921 tattggtctt acaagaaatc agacattatt taaaatacaa ttccctttag cacttcctat
     4981 aataatgggt ggagttagaa tatctgcagt tacagctgtt ggtttgatga ctcttgcagc
     5041 atttataggt gctggtggtt taggatattt agtattttca ggggttcaaa ctgtaaataa
     5101 taatatgatt ttagcagggg caatcccatc atgtataatt gcactgttag ttgattattt
     5161 atttagtaaa attgaagttg cagttactcc aaaaggatta aatccaaaag cacctaagaa
     5221 gaattatata gcacttaaag taatatctgt agtggttgtt gtttcaatgt tgttcgttgt
     5281 ttttagttct agtttttcaa gtaaaaaaga tacaataact attggttcta aagattatac
     5341 agaacaactt atacttggta atgtttatgc tgaattagta gaaaaaaata cgaatcttaa
     5401 agttaagaag aatttaaatc taggtggttc atctgttgcg tttaatgcaa ttaaatctgg
     5461 tgagttagac atgtatgttg actatacagg tacacttttg gtaaatgtaa tgaaacatgc
     5521 tccgattaaa gatgctgatg aagcatataa tgttgtaaaa gatactatgg aaaaagaaaa
     5581 tcagttaact ttacttgacc ctcttggatt taataatact tacacattag ctatgatgcc
     5641 tgaaactgct gaaaagtatg gaataaatac tatttctgat ttgactaaat atggtaaaga
     5701 atttacattc tcaccaactt tggagtttga aaatagagaa gatggtcttg tagggctttc
     5761 tagagattat ggtttaaaat tcaaagatgt aaaagcaatg aatggaagtt taagatatac
     5821 agctttagat aataatgaat ctcaggttat agatgcattt ttaactgatg gtcttattaa
     5881 aaagtttaat cttaagatat tagaggacga taaaaatttc tttgtaccat attatgcagc
     5941 accattagta agagaagata ctttgaagaa atatcctgag ttagaaaaag ttatgaatat
     6001 gctttcagga aaaataaatg aagaaactat gagagaactt aactatcaag tggatgaact
     6061 tggtaaatca ccagaagaag ttgctcattc attcttagtt aaagaaggct tagtataaaa
     6121 aattaatagt ttatatcaaa aaatttaaga gagtgtctca aaatgaactt tttagttcat
     6181 gaggcactct tttttatatg aaatcaaata tattttatca tttcaacaat aaaaaagaag
     6241 attatcttta ttttgagaca gtctcttatt tttatgtata ccaaaaccat acctacgatt
     6301 tttatttata attaagtttc aaatagaatg aacaagtaca accaattttg aatataaata
     6361 tagtaaatgt gcttatatac gattttattg tctggttgat tgatattgat agatagcttt
     6421 ctttattgaa tcgaagggag ggggaatttg ttatggataa ctataagaaa gtgaagcaag
     6481 tattgtggat aattttattt gctaattttg cagtagcatt attaaaaatc attattggta
     6541 atcaaataaa aagctatagt atgacagcag acggatttca ttctttgtca gatggagcat
     6601 ctaatatagt ggggcttata ggtattttct ttgcatctaa accaaaagat aaaaatcatc
     6661 cttatggaca taaaaagttt gaaattataa ccagtttatt tataagtggt atgttgtttg
     6721 ttatagcaat taagataatt cttagtgcag tgttaagaat agctaatcca gttgtacctg
     6781 ctataactat agaaagttta attgctttaa ttattacact ttttataaat atttttgtat
     6841 gcatgtatga atatagaatt gggactaaac taaatagtta tgttttgatt tcggattcat
     6901 tgcatacaag aagtgatata tttgtgtctc taggtgtatt ggttacttta gttggtgtta
     6961 agttaggatt tccagtaata atagaatcta tagttcctat tattatttct gcattcataa
     7021 tttattcggc ttatggaata tttaggccat ctattggaat tttggtcgat agagtggctg
     7081 tagatgagga ttatataaaa gaaattgtgt ttgaatttaa tgaggtaagg gatgttcaca
     7141 atatcagaag tagaggtagc aaaagttcta tttatattga catgcatgta atggtagacc
     7201 catttataag cgtagagcaa tctcatgatt taacacataa aattgagaaa caaatacaag
     7261 aagaaattaa tgaaaatgct caagtaatag tccatattga acctttttat agtttttaac
     7321 ttagctataa aatacaagtt ataaaaaaat ataatttata aaaaaaatat tataccataa
     7381 ataatttata aaaattattt atggtataat attttctata aagaaattaa gtcttaatat
     7441 atctaaggag gaatttacaa tgagatacgt tgaatatgga aagacaggga aaatggtttc
     7501 agtagttggc tatggaggat taaggtttga tttagaaaaa agtaatcaag aaaatgctga
     7561 tttagtcaaa tatgcgtatg aaaaaggtat taattacttt gatacagcac cagggtattg
     7621 tgatgacaga agtgaagata tatttggact agcctttaga caaatgataa aggaaggtaa
     7681 gaccgatttt tatgtatcaa ctaaagaaaa gccaaaagca tgtgatacat cagaaaaagc
     7741 tatagaggct gtaaaaaaat caatagaacg attaggcgta tctaaaataa atttttatca
     7801 tgtttggaat attagaaaga tggaacatta cgaactagca atgagacctg gaggtcaata
     7861 tgaaggatta ttgaaatgta aagaagaagg attgatagac catatagtat tttcatctca
     7921 tcaaccagga gatgaggtta tagatattct aagtgaaaat aagtttgaag gtgtaactat
     7981 gggaataaat atattaaact ttccatatag gctaaaaggt gccaaatatg ctgttgataa
     8041 tggatatggt gttgtggcaa tgaatccatt aagtggtgga actattccaa agtataataa
     8101 agaactttct tttctatcaa aagatggcga aagtactata gaatcagctt taagattcaa
     8161 cataggaatt tcacaaataa gtatatcttt gatagggttt aataaaaaac aagatataga
     8221 tgatgcatgt aaaattgcag acgaaaatag aatttattca gatgaggata tacatgatat
     8281 tgagactaga ttaaataaga atatgaacga aatatgtaca ggttgtggtt attgtaaagt
     8341 atgtccaaaa ggtattaata ccccagccta tatgcttttt tataatgaaa agcagatgtt
     8401 taaaaagagt gatgaagaaa tgacaaagtt agtttatggc ttaggacatt ggaattacac
     8461 aatgaatagt aaggcaaaag ctaaagaatg tatatcttgt ggaaaatgtg aagttgaatg
     8521 tactcagcat ttacctataa tagatagatt aaaagaaatt aaaaaatggg aagaagatgg
     8581 agctaatagt gttaaagtct aattaatttt atactatatt tgaaatttgt atagtattta
     8641 gtaatgtttt aaaatatagt tttaatattt aaggagacag attataattt tgtctcctta
     8701 aattatgctt taatttgtta tagtttaatt ccataagtaa cattgtatat ttttatataa
     8761 agtttttcaa atattttaca ttaatattgc ttacaaattc tcattttata caacactaat
     8821 ctatgttata taaacaatac ttgactgatt ataacatcaa taaagtaaat attgtggttg
     8881 taaataaaga gttattcaat tacaataaaa caagtaagaa cattatgaag atggctatta
     8941 aatataagga ataaattgta ggtgtcagca aatcctatat aatgaatgga tggtgatata
     9001 ttgatgagta aaatattaaa atataaaaac gagatgtttt tgtttatatt agttgtaata
     9061 acttacttaa taataactaa gatatttttt tctaagacta ctatatttta tgatttaaac
     9121 aatacttatg atgtattgtt agatacagat acaggagttt tattcaattt aaatgtattt
     9181 gctataagtc aagacaatag taagcatata ttgttttcag caattatatc aatttttgca
     9241 tatccaatat acctattttg tacttcaata gcaaatcctg ggacaacaga ttttaatagt
     9301 gcctatgggt ttggactaat ttgtctacaa ataataacta gtgctatgtc aataacttta
     9361 gtctttaatc atattaaaaa gataaaaatg cagagattaa ctttaatttt acttactatg
     9421 ataatgatat tttcttttcc acagttattt atgactttaa atgtagagag atttatatat
     9481 agccaatttt cattaatatt tttcatagtt attgcaaata agatgaaagg aaaaaattct
     9541 tatttggtag aattagcagc tattcctcta tttgg
//
LOCUS       DAYATN010000138         9357 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.138, whole genome shotgun sequence.
ACCESSION   DAYATN010000138 DAYATN010000000
VERSION     DAYATN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.138"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1152
                     /gene="bclA2"
                     /locus_tag="ACGI8A_003103"
     CDS             <1..1152
                     /gene="bclA2"
                     /locus_tag="ACGI8A_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HFH0181085.1"
                     /translation="GATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIGA
                     TGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGLV
                     GPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGATG
                     ANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTGP
                     TGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFNS
                     SFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAKA
                     VNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(1583..2338)
                     /gene="dapB"
                     /locus_tag="ACGI8A_003104"
     CDS             complement(1583..2338)
                     /gene="dapB"
                     /locus_tag="ACGI8A_003104"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFH0181086.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            2778..3767
                     /locus_tag="ACGI8A_003105"
     CDS             2778..3767
                     /locus_tag="ACGI8A_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFH0181087.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(4219..4935)
                     /gene="dapD"
                     /locus_tag="ACGI8A_003106"
     CDS             complement(4219..4935)
                     /gene="dapD"
                     /locus_tag="ACGI8A_003106"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFH0181088.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(5159..5908)
                     /gene="dapB"
                     /locus_tag="ACGI8A_003107"
     CDS             complement(5159..5908)
                     /gene="dapB"
                     /locus_tag="ACGI8A_003107"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFH0181089.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(5975..6862)
                     /gene="dapA"
                     /locus_tag="ACGI8A_003108"
     CDS             complement(5975..6862)
                     /gene="dapA"
                     /locus_tag="ACGI8A_003108"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFH0181090.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(6979..7980)
                     /locus_tag="ACGI8A_003109"
     CDS             complement(6979..7980)
                     /locus_tag="ACGI8A_003109"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFH0181091.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     regulatory      complement(8153..8327)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(8405..9286)
                     /gene="dapA"
                     /locus_tag="ACGI8A_003110"
     CDS             complement(8405..9286)
                     /gene="dapA"
                     /locus_tag="ACGI8A_003110"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFH0181092.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         3230 a         1537 c         1387 g         3203 t
ORIGIN      
        1 ggagcaaccg gagcaaatgg agtaacaggt gctacaggcc caacaggaaa tacaggagca
       61 acaggtccaa ccggaagtat aggagcgaca ggagcaacag gaacaactgg ggcaacaggc
      121 ccaataggag caaccggagc aacaggagca gatggagagg taggtccaac aggagcagta
      181 ggagcaacag gtccagatgg tttggtaggt ccaacaggcc caacaggccc aaccggagca
      241 actggagcaa atggtttggt aggcccaaca ggcccaaccg gagcaactgg agcaaatggt
      301 ttggtaggtc caacaggagc gacaggagca acaggagtag ctggggcaat aggtccaaca
      361 ggagcagtag gagcaacagg cccaacggga gcagatggag cagtaggtcc aaccggagcg
      421 acaggagcaa caggggcaaa tggagcaaca ggcccaacgg gagcagtagg agcaaccgga
      481 gcgaatggag tagcaggtcc aataggtcca acaggtccaa ccggagcaaa tggagtagca
      541 ggagcaacag gagcgacagg agcaacaggg gcaaatggag caacaggccc aaccggagca
      601 gtaggagcaa cgggagcaaa tggagtagca ggagcgatag gaccaacagg cccaactgga
      661 gcaaatggag cgacaggagc aacaggggcg accggagcaa caggagcaaa tggagcaaca
      721 ggtccaaccg gagcgactgg agcaacagga gtgttagcag caaacaatgc acaatttaca
      781 gtgtcctctt caagtttagt gaataataca ttagtgacat ttaattcatc atttataaat
      841 ggaactaata taacttttcc aacaagtagt actataaatc ttgcagttgg agggatatac
      901 aatgtatctt tcggtatacg tgccacactt tcacttgcag gatttatgtc aattactact
      961 aactttaatg gagtagctca aaataacttt attgcaaaag cagtaaatac acttacttca
     1021 tcagatgtaa gtgtaagttt aagcttttta gttgatgcta gagcagcagc tgttacttta
     1081 agttttacat ttggttcagg cacgacaggt acttctgcag ctggatatgt atcagtttat
     1141 agaatacaat agtttatatg atgaaaagat tataaaataa taaacaatat gatttcatat
     1201 accaataaat agaataatat ttaataggtt tatatgatga aaagactata aaataataaa
     1261 caatatgatt tcatatacca ataaattagg ataatattta ataggtttat atgatgaaaa
     1321 gattataaaa taataaacaa tatgattcca tataccaata aataggataa tatttaatag
     1381 gtttatatga tgaagagatt ataaaataat aaacaatata attccatata ccaataaata
     1441 agataatctt taagaaaaat gattatattt aaaaggtaaa cataaaattt ataaactttt
     1501 agaatagaaa tctcaatcaa ttagatattc aaattgatga atatgaatac ctaaatagat
     1561 tgagatttat tatgtacaaa acttatccta atatatctga cattttataa agcccagatt
     1621 gcttaccaat taaaaattta gctgcaacta tagaaccgtt tgcaaatact tcatcagatt
     1681 gagaatgatg gcttaatgta agaacttcat tatcacctgc aaatacaaca tcatggtcgc
     1741 tgattatatt gccacctctt atagagtgta ttccaatttc atttggtttt cttttacaat
     1801 ctctaccata tctaccatgt gtattttcac tttcaggcaa gacttctcta attgcattag
     1861 ctatcatagt agcagttcca ctaggggcgt cttcttttct attgtgatgt ttttctatga
     1921 tttcaatatc aaaaccttct aacatctttg aagcatcctt aactaatttt attaatatat
     1981 tcactccaag agatgtattt gatgataata atataggaat gttttttgat gcttcttcta
     2041 ttttatttaa gtcattttca tcatatccag tagtagctaa tactacaggt gtattatttt
     2101 ttagagcata tgacaatatt ggctctaaag tctcatgatg tgaaaagtct attattacat
     2161 cagttttttc agttacatca gtcattgttt tgtaaagctt tgtatcacta tgatgacctt
     2221 tttcatcagc agacctagct acaccacaaa ccaactctaa ttcattatct ttttttacac
     2281 attctgctaa aactcttccc attgttccat tatagccact aattataact ttcaacataa
     2341 attcctccta tgaaattaat aaaaatttgt tatatttcta taagtttatt ttatcactaa
     2401 tataatttat ataaaataat attagtgata ataagcttac aatttatctt acattaaaaa
     2461 atttaagtgg atttttcaaa atatagataa atttcttttt cattgtataa atgataaaaa
     2521 tatatttgac ttcataccaa aaagagtgcc ccatgaacta aaaagttaat tttgaaatac
     2581 catattcata tatcatatga tttatttgga aatcaatatt aatacattaa aataactaaa
     2641 tacatgtatg cataaagatg tataaaaaat atataaacat atagtattaa ttttaaattc
     2701 aacaatttaa ttgttaaaag tgttattatt aaattagaaa tatcttaact taatatactg
     2761 agatgggagt gatagatatg ttaaaagaag aaaaaactta tcaagagaaa gacgcaaaag
     2821 tattcaatgg agtggcagct ttgataatga ttttattatt tttagtagtt tcaataggta
     2881 tcatggtttt tgggatagta aaacttgata gtggatttac tggaataggt tcacttatgt
     2941 taattttggg acttgtattt atagtagtag attttatcct attttttggg ctaaaaatga
     3001 ttaatcctaa agaagctata gttctagttt tgtttggtaa ctattatgga actataaaaa
     3061 aagaagggta ctattgggtg aatccatttt gttcagcaat aaacccagca gtatcaagaa
     3121 ttaatgtatc gaaatcttca tctgatgaaa aggtagatat atcaactaat tcaaggggca
     3181 aaaaagtatc tttaaaaaca atgactctta ataatgaaaa gcagaaagtc aatgatttat
     3241 tgggaaatcc aattataata ggagtggttg taatttggaa ggttatagat gcaacaaagg
     3301 cagtatttaa tgtagataat tataatacat tcttatctat tcagtgtgat tctactattc
     3361 gcaatgtttc aagactttat ccatatgatg tatctgaaga tggagatgaa aaatctcttc
     3421 gtggtagtag tcaggaaata gcagatagat taaaagatga attacaatca agggttgata
     3481 ttgcaggaat tgaagtgtgt gaagtgagaa ttactcatct ttcttatgca ccagagattg
     3541 cagcagcaat gttacaacgt caacaagcag aggctataat agcagcacgt aaaaagattg
     3601 ttgagggggc tgtaagtatg gtagagatgg cattgactaa tttaagtgaa aataatgttg
     3661 tgactttgga cgaagaacgt aaagcagcta tggtaagtaa tctgttagtt gtgctttgtg
     3721 gcaataaaga tgcacaacca gtggtaaaca gcagttctat ttattaaatt ataaaaagtt
     3781 taatagttta ttttattgat atatagaaaa tttagtagtt cattttatta aattatagaa
     3841 agattaatgg tttgctttat tgaattataa aaagtttaat aatttattct attgatatat
     3901 agaaaattta gtagttcatt ttattaaatt atataaagat taatggtttt cactttatta
     3961 aattataaaa agtttaatag tttattacat tgatatattg aaaaattgaa ggaaataaaa
     4021 atacttgtca gcaagaaata aaaatgatat aagaatgaat aagagaattg taaaaaaata
     4081 aaatgattat aaataaaaga taaaaatatt gtaaaaaaga gggtgcctct ggtgaattaa
     4141 attcacacag agacaccttt ttaattaatt ttttaaatta gtatattatt gtttaattaa
     4201 tagtatagta ctatttaatt aatctaaatt tctaaggtct tccataagct tagttttgtc
     4261 agccgttttt tcatctttca tttttataac tttagcagga gaaccagcaa caacggcacc
     4321 agcttctaca tcagtagtta caacagcacc agctgcaaca acagcacctt taccgattct
     4381 aacaccttca agaataactg cattagctcc tattaaaaca tcatcttcaa ctataacagg
     4441 agtagcagaa ggtggctcta aaacaccagc aacaacagca ccagcaccta agtgaacatt
     4501 tttaccaaga gttcctctag ctccaataac agcattcata tctaccatag aaccttcacc
     4561 aataacagct cctatattta taacagctcc catcattaca acagcatttt tttctatagt
     4621 aaccatgtct cttattattg cacctggctc aattcttgca tgttcactta aagtattttt
     4681 tagaggaata gcagaatttc ttctatcata ttctacatga gtatctgtta tatcatttcc
     4741 atgtttatct aatacttcca ttatatcctc atagtcacct acaaatatat aagaaccatt
     4801 acttccgaat accttaaatt tatcagttga ttggaagtgt aaagagttag tatttacata
     4861 taccttaaca ggtgttttct ttttaacttc ttttatatac cttgctattt cataagcatc
     4921 gtttaaattt accatttgtt aattctcctt gtatttcatt atatttcttt tattatatac
     4981 ttatttgtca attttagtat atacttactt cttattattt atattttatt ttagttagtt
     5041 tttattgtat agacatagta ttaaataaat ttattatata tgtttgattt gtattgcgta
     5101 ttattatatc tatattgcat atgtttaatt tatattgtgt attattatat ttatgctatt
     5161 atcccaacat gctgtccata ttataaaatc ctggtttttg cttaactaag tatttggcag
     5221 cagctataga accacttgca aagataccac gagattgagc ttgatggctt attgtaagaa
     5281 cctcattgtc tccagcaaat agagcatcat gttcgccaac aatatttcca cctcttatag
     5341 catgaatacc aacttcattt ggttgtcttt ttgcactacg accatatcta ccataattat
     5401 tttcaacgtt tggcaaaacc tcttttatag cgttagcaat cataacagct gtgccacttg
     5461 gggcatctac ttttctgttg tgatgttttt ctattatttc tatatcaaat ccttctagta
     5521 atttagctgc atctttaacc aattttacta atacatttac accaagagac atattatatg
     5581 attggaatac aggaataact ttagccgctt cacgtatttt gttcatttcg tcatcattat
     5641 atccagtagt agcaattact aaaggtgttt tagtctttaa aacgtaagat aagatattat
     5701 ctaaattaga gtgatgagaa aaatctataa ctacatcagc ttcctcaact atagtactca
     5761 tatcttcata aattttaagg tcgccttcat agctatccag acctctagct gcaacacaaa
     5821 ccaattctaa ttcattatct tctcttatag tatcggctaa aatcttaccc atttttccat
     5881 cataaccagt tgctataact tttaacataa tttcctccta tgattaaggg ctaacttttg
     5941 accaaactta ctttttacca tgcattaatc tttactatgc attaactttt agcccaaagt
     6001 taactaattc tttctttaat acttctaaat ttgctgggtc catttctgct agaggtaatc
     6061 ttaaatctcc aacattgaat cccaataaat tcattgcagt cttaactggt ataggattta
     6121 cttctataaa taaagctgcg attagagcat ccataccaag ttgtaatttt ctagaacctt
     6181 caatatcgcc ttcaaagaat ttagctacca aatcgtgagt ttcttgaggg caaacattag
     6241 caagtactga tataactcct tgacctccta aagataataa aggaagtata gtatcatcat
     6301 taccagagta gatagcaaag tcatctggaa ctaaactagc tatttctgca acctgtccta
     6361 ggtctccact agcttctttt acagcaacta tgttatcaat tttagcaagt tcaactataa
     6421 ggctaggact tatatttaca caagttcttc cagggacatt gtaaagtata attggaagtt
     6481 taacactgtt agctattgtt tcaaaatgac gcttaagtcc agctttatta gctttattgt
     6541 aataaggtgt tattattaat agagcatcta ctcctaattt ttcagcttct tgacttaagt
     6601 taactgagtg catagtatca ttagaaccac ttccagctat aacaggaact cttttattaa
     6661 ctttttcaac aacatactta atagtagcaa gttgttcttc atcagtcata gtattagctt
     6721 caccagtagt accacaagat actatagcat cagtaccatt agctatttgg tattcaacta
     6781 attcacctaa tttgtcaaaa tcaactttgt tatcctttgt aaatggtgta actagagcaa
     6841 cagcagaacc tttaaatatc atataaaatc aatcccctct tttttatttt tgtgttttat
     6901 attacagtta cttctaatgc ttgtttttga gttactatta atattttaca aaattatata
     6961 tttatacaag tttgtatatt atactaaatc gtatgataaa gccatttcag ctatttgaat
     7021 tgcgttagtt gcagcacctt ttcttatatt atcagcaaca acccaaagat ttacaccatt
     7081 atcaacacta aaatctcttc ttattcttcc aacaaacact tcatctctat catcaaattc
     7141 tacagcagtt ggatattcgt ttttagcagg attatcaact actacaacac ctggagcttc
     7201 ttcaagtgtt tttactaaat catctaactc aaatggattg ttaaactcaa catttataga
     7261 ttcactatga ccatttttta ctggaactct tacagtagtt gcagttattt ttaaatcgta
     7321 gttatcaagt attttcatag tttcatttac cattttcatt tcttctttag tgtatccatt
     7381 ttcagtgaat gaatctatat gaggaagaca gttgtaagag atttgatgag ggtagaatcc
     7441 agtaggatta ccgttcattc ctttctctaa atcttcaaca cctttaacac cagaaccaga
     7501 tacagcttgg taagtagagt aaacaactct cttaattttg tatttatcat caagagcttt
     7561 aaggggaacc atagcttgta tagtagagca gttaggatta gctattatac ccttatgatt
     7621 ttttatggct tctggattaa cctcaggaac tactaaaggc acatctggat tcattctcca
     7681 ttgactagag ttatctacta ctaagattcc atttgaagca gctattggag catacttttc
     7741 actaatactt ccaccagcag agaataatgc tatttgtatg cctctatcag taaatgaagt
     7801 ttcctttaat tcttctacta tatattcttt accacaaaac attactttag aaccagctga
     7861 tttagaagaa gagaataaaa atagattttc tattggaaaa tttctttctt ctaatacttt
     7921 taaaaatgtt ctacctacca ttccagtagc accaactatt gctacattta cttttttcat
     7981 ataaattcac cccaatttta aattattaaa acattatttt attttagtta tacaagtaag
     8041 atatgttaat atttttatat atccaaaaac tttaatatta tattaacaac tttaattata
     8101 tacaaaaaac gtacaaaggt atactttgta cgtctaattt atcactcaaa gtaaaccctg
     8161 tagctcatca cctacaaaaa taagtgacag tattacacat gttatgtgca atcccagaaa
     8221 atattattgc aagaaatatt ttcttcggct gtaaatcctt tttccaatca ttaacacctg
     8281 ccagctacta ttggatactc ttagttacag ctacctctac cctaggttaa taggatatta
     8341 aaatttgatt attctaataa taatacatat ttaagaaaaa atcaacttat ttatataaat
     8401 attgctaaaa tttaaatcca aaatttgtca attcttgttt taacacatta agattagttt
     8461 cttccatttc agctagagga agtcttaaat caccaacatt aaaacctaaa atgttcatgg
     8521 cagttttaac aggaactgga tttacttcta taaataaagc agcaattaaa gcatccatat
     8581 caagttgtaa tttttttgag ccttcaatat caccttcaaa gaatttagtt actaaatcat
     8641 gtgtttcttt aggacatatg ttagcaagta cagagataac accacttcca cctaatgata
     8701 atagtggtag tatagtatca tcattaccgg agtatatagc aaagtcttta ggtactaatt
     8761 tagctatttc tgcaacttga gctaagtctc cacttgcttc cttcacagca actatattat
     8821 ctattttagc aagttcagcc acaacagatg gctttatatt cactttagtt cttcctggta
     8881 cgttgtataa aataattggc aacttaacgc tatttgctat tgtttcaaag tgaagtttaa
     8941 gacctttctc attagtctta ttatagtaag gagttattat taaaagacca tccaccccta
     9001 atttttcagc ttcttgactc atatgaacag acaacatagt attgtttgaa ccagtacctg
     9061 ctataacagg tattctctta ttaacttttt caactgtata ttttattaca gcaaaaattt
     9121 cagattcact catagtagtc gcctcacctg ttgttccaca aacaactagt gcatctgtgc
     9181 cattctcaat gtgatattcg attaattcac ctaatttttc aaagttgaca ttattatcct
     9241 ctgtaaatgg ggtaactaaa gcaacagcag aacctttaaa taacatataa tcaaatctcc
     9301 ttttttatct aaatttaatt ttttaagtac aataataatt atagtacaaa ttaatta
//
LOCUS       DAYATN010000139         9261 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.139, whole genome shotgun sequence.
ACCESSION   DAYATN010000139 DAYATN010000000
VERSION     DAYATN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.139"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            93..1853
                     /gene="prdR"
                     /locus_tag="ACGI8A_003111"
     CDS             93..1853
                     /gene="prdR"
                     /locus_tag="ACGI8A_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HFH0181093.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            2282..4162
                     /gene="prdA"
                     /locus_tag="ACGI8A_003112"
     CDS             2282..4162
                     /gene="prdA"
                     /locus_tag="ACGI8A_003112"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HFH0181094.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            4243..4527
                     /locus_tag="ACGI8A_003113"
     CDS             4243..4527
                     /locus_tag="ACGI8A_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBO2463/CBO2479 domain-containing protein"
                     /protein_id="HFH0181095.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            4565..5290
                     /gene="prdB"
                     /locus_tag="ACGI8A_003114"
     CDS             4565..5290
                     /gene="prdB"
                     /locus_tag="ACGI8A_003114"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5015..5017,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HFH0181096.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            5370..6128
                     /gene="prdD"
                     /locus_tag="ACGI8A_003115"
     CDS             5370..6128
                     /gene="prdD"
                     /locus_tag="ACGI8A_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HFH0181097.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            6143..6610
                     /locus_tag="ACGI8A_003116"
     CDS             6143..6610
                     /locus_tag="ACGI8A_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFH0181098.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            6637..7110
                     /locus_tag="ACGI8A_003117"
     CDS             6637..7110
                     /locus_tag="ACGI8A_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFH0181099.1"
                     /translation="MGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSEHNE
                     WKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIMGAD
                     GLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK"
     gene            7130..8137
                     /locus_tag="ACGI8A_003118"
     CDS             7130..8137
                     /locus_tag="ACGI8A_003118"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HFH0181100.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            8257..9138
                     /locus_tag="ACGI8A_003119"
     CDS             8257..9138
                     /locus_tag="ACGI8A_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFH0181101.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         3628 a         1143 c         1862 g         2628 t
ORIGIN      
        1 taatatttat ttaataaata attattataa tatgctagaa taaatatata taaataagtt
       61 ttgatgaggt actactataa aggggggact ttatgttttc gataccagaa gtaaaaaaag
      121 tagaagaggt aatggataca aaatttacaa ctatagacga agacacaaga attgaagatg
      181 caataaaaga aatgataaaa agcaacacaa aaactctaat ggtaatagat tcatctgacc
      241 aattaaaagg cattatatcc atgacggata tacataatct ttatgagatg cataaaaagt
      301 atgaaggtca accagtaaaa cttataatga agaaggatgt aatatatgtc aacgaaggtt
      361 taaccttaga tgaatgtaga gatataatga tattaaaaaa tatagggatt cttcctgttc
      421 ttagagataa taaaattatt ggagttctaa aacaagagca cattagggac tatctatata
      481 tgcatttaga agattatgga ttaactttga agtatattat aggtcaaatt aaagaaggaa
      541 tttgtgctat aaataacgaa ggtgttgtta tcttgtggaa taagtttatg gaggaaaggt
      601 atgatataaa gtctgaagat atagttggtc gaccgatgaa tgaatttttg gaaaatacta
      661 tttcagaaaa agtactaaat agtaaagttg gaatgagtga cttgtatttt acagataaaa
      721 aagaaaatat gtatgcatta gttcatgcaa atcctatatt ttataaagaa gaatttatcg
      781 gtgttgtatg tacagaagtt gatgttacag aagctaagat attagctctt gaacttgaaa
      841 aagtaaatga tacattaaaa tatctaaaga acgaagtcaa aaatttatct aaaggtagtt
      901 ttgataagat tttaggtaaa agttataaat tggaaaaatc gaaagcgatt gcaaaacaag
      961 tggctagaac aaatagtagc atatttattt ggggcgaaag tggtacaggt aaagaagtat
     1021 ttgcaagagc tattcatgat tatagtgaga gaaaaggtca atttatacct gttaactgta
     1081 gtgccatacc aaatgagtta tttgaaagtg agttttttgg gtatgaatca ggtgcgttta
     1141 caggagcaag taaaaagggt cgaataggta tatttgaact tgcaaaagat ggaactgtat
     1201 ttttagatga aattgccgat ttacctctta gtatgcaagc aaagttgctt agagtattac
     1261 aggaaaaaga aataagaaga gttggtggag ataccacaat aaaaataaat cctagaataa
     1321 tatcagctac caataaagat ttgagtaaaa tggtaaaggc agaaaaattt agagaagatt
     1381 tatactatag attaaatgtt gttgaaataa agattcctcc acttagagag agaaaagaag
     1441 acataggatt attggttcat agctttttag aggagatatg taaacaaaat aataagcctg
     1501 ttcttacaat tagcaaagat gtaatagata tatttcagaa ttatagatgg aaaggtaata
     1561 ttagagaatt aaaaaatacg attgaaaata ttgttgtatt gtcacaaaat tctaaaatag
     1621 atgtggatga tgtaccaagt tatatgatgg attctacaaa taatagtact gaagaagaag
     1681 agtatccttt agatttaacg aaggcaactc aaaaaattga aataaagaat ataaccaagg
     1741 cacttaaaat gtctaatgga aataaagcta aagcagctaa gatattaaat atacccagaa
     1801 caactttgta ttataagata gaccaataca aaatagatgt gtctaaaata tgacacatga
     1861 ctaaattttg acaaaatgat aaactttaat ttaaaaccta gtaaagccaa cgcttatata
     1921 agtgttgtgt atatagatgt gttaaaaaaa atgaaaaagt gttgattaat tgacactttt
     1981 tttgtttgag aaaatgttta caaatacaaa aaataacata ttttaaataa atatttatat
     2041 aatatttggc taaaaatagc gatagatgaa gaatctatta tagaaaaaat ctttttgtta
     2101 ataagttggc ataggaattg cttataatat aaagtgactt taagtgagaa aaaaataata
     2161 ttaatcataa caaattatgt aggttttaat ttacttaatt tctcagttaa ggggttacct
     2221 gttgaggaat tgagatttaa aataataaat aagaaaataa tgtaaggagg tattttatac
     2281 tatgtcaata actttagaaa cagctcaagc ccatgcaaat gacccagcag tttgttgttg
     2341 tagatttgaa gcgggaacaa ttatagcgcc agaaaactta gaagatccag caatatttgc
     2401 agacttagag gattctggat tattaacaat accagaaaat ggattaacta taggtcaagt
     2461 actaggagct aagttaaaag aaactttaga tgcactttct ccaatgacta cagataacgt
     2521 agaaggatac aaagcaggag aggctaaaga agaagtagta gaagaaacag tagaagaagc
     2581 agctccagta tcagaagcag cagtagttcc agtaagcaca ggagttgcag gtgaaacagt
     2641 taaaatacac ataggtgaag gtaagaacat aagcttagag atacctttat cagtagctgg
     2701 tcaagcagga gttgctgctc cagtagcaaa cgttgctgct ccagtggcaa gtgcagcagc
     2761 agaagtagct ccaaaagttg aagaaaagaa acttttaaga agcttaacta aaaaacactt
     2821 taaaatagat aaagttgaat ttgctgatga aactaaaata gaaggaacta ctttatacat
     2881 cagaaacgca gaagaaatat gtaaagaagc taatgaaact caagagttag ttgtagatat
     2941 gaagttagaa ataataactc ctgataaata tgaaacttac agtgaagctg tattagatat
     3001 acaaccaatc gctactaaag aagaaggcga attaggttca ggtataacta gagttataga
     3061 tggagctgta atggtattaa ctggtacaga tgaagatgga gttcaaatag gtgaatttgg
     3121 ttcttcagaa ggtgagttaa atactactat aatgtggggt agaccaggtg ctgctgacaa
     3181 aggtgaaata ttcatcaaag gtcaagtaac aataaaagca ggaactaaca tggaaagacc
     3241 aggaccttta gctgctcacc gtgcatttga ctatgtaact caagaaataa gagaagcatt
     3301 aaagaaagtt gacaactctt tagtagttga tgaagaagta atagagcaat acagaagaga
     3361 aggtaaaaag aaagttgttg ttataaaaga aataatggga caaggtgcaa tgcatgataa
     3421 cctaatatta ccagttgagc cagttggtac attaggagct caaccaaacg ttgacttagg
     3481 aaacatgcca gttgtattat ctccacttga agtattagat ggtggtatcc atgcattaac
     3541 ttgtatagga cctgcatcaa aagaaatgtc aagacattac tggagagagc cattagtaat
     3601 aagagctatg gaagacgaag aaatagattt agtaggtgtt gtatttgttg gttctccaca
     3661 agtaaatgct gagaaattct atgtatctaa gagattaggt atgttagttg aagctatgga
     3721 agttgatgga gctgtagtaa ctactgaagg tttcggaaac aaccatatag atttcgcatc
     3781 tcacatagag caaataggta tgagaggtat accagtagtt ggtgtaagtt tctcagctgt
     3841 tcaaggtgct ctagttgttg gtaataaata catgactcac atggtagaca acaataagtc
     3901 taagcaaggt atagagaatg aaatattatc taacaacact ttagctccag aagatgctgt
     3961 tagaataatg gctatgctta aaaatgctat agaaggtgta gaagttaaag ctcctgaaag
     4021 aaaatggaat ccaaatgtta aattaaataa catagaagct atagaaaaag ttacaggaga
     4081 aaaaatagta ttagaagaga atgagcaatc tctaccaatg agtaagaaga gaagagaaat
     4141 atacgaaaaa gacgaaaact aaaatttaga tactaactaa gatttagaca ataacataaa
     4201 tttagatcca agtttaaaac agtatagaga ggtagaatta taatggatag gttaaaatat
     4261 atctcatcag agacttttta tgaaggtatt atagttgaca tcaaaggcgg tggagtgact
     4321 atagacctaa aaggtagact tggacaattt aaaataccta atagaatgct tataactgac
     4381 tacgaactta agataggtca agaagttgga ttcatgctaa gctatcctga ggtattaagt
     4441 ccagagccta atcaagagta tgtagaaaat attaggagag aaaaagaaaa acaagcagaa
     4501 atgcaatcaa aacaagaaga aaaataataa ataatcgttt gaaaatagaa gggagaggaa
     4561 atatatgagc cttacaacag tacaaggact tcaatctgaa atattcgttc caataacacc
     4621 tcctccagtt tggactcctg taactaaaga attaaaagat atgactatag ctttagctac
     4681 agcagctggt gttcatttaa aatctgacaa gagatttaat ctagcaggag atactacatt
     4741 tagagctata cctaatacag ctacagttga tgaaatgatg gtatcacatg gtggatatga
     4801 caatggagat gtaaacaaag atataaactg tatgttccct atagatagat tacatgaatt
     4861 agctgcagaa ggatttatca aaggagtagc tcctatgcac tatgcattca tgggtggtgg
     4921 aggaaaccaa catgtcttca ctgaagaaac tggtcctgct atcgctgcta aacttaagga
     4981 agagggagta gacggtgtag ttatgacagc tggctgaggt acttgccata gaactgccgt
     5041 gatcgtgcag agagcaatag aggaagctgg aatacctaca ataataatag cagctcttcc
     5101 tccagtagtt agacaaaacg gaactcctag agcagttgct ccattagttc caatgggtgc
     5161 taatgctggt gaaccacata atatagaaat gcaaacacat atattaagag atacattaga
     5221 gcaattagtt gcaataccat ctgctggtaa gatagttcca ttaccatacg aatataaagc
     5281 tcacgtttaa aatgtaattt atatttgaaa atatagaacc cttctcctac acggttagga
     5341 gaggggtttt aagaacaaag gtggatatta tggaagaaaa aataatgaga agacttgtta
     5401 taaaaccgtt tcatatgaat gaagttaatt tcggttcaaa aacttctata aagaaagatg
     5461 tacttacaat agatttatca agtatagatg aaataaaaga aagagaagag ttaataacag
     5521 acataaaagt agacattata aaacctggag attatgacag agaaattaat actattatgg
     5581 acattatacc aatatcaaca aaagttttag gcagactggg agagggtatt acacatactt
     5641 taacgggtgt atatgtaatg ttaactggtg cagatgaaga tggaaatcaa atgcatgaat
     5701 ttggttcttc agaaggaatt ttgaaagacc aaatggtatt tgggagatat ggaactccat
     5761 cagttgaaga ttatataatc catgtagatg taactttaaa gggaggttta ccttttgaaa
     5821 gaacacttcc acttgcagca ttcagagcat gtgatgattt tatacaagaa ataagagcgt
     5881 ctttgaaaat ggaagacgga agaaatgcta ctcaagttcg tgaatatttt gacaaaattc
     5941 gaccaaatgc aaaaaaggtt gtcatagtaa aacaaatagc aggtcaaggt gctatgtatg
     6001 acaatcagtt attctcaaag gaacctagtg gatttgaagg aggaacatca attatagata
     6061 tgggaaatgt acctatgatt atatctccta atgaatacag agatggtgcc cttagagcta
     6121 tgacttaaga gaggtggata ttatgggaat aggaccttca acaaaagaaa catctcttca
     6181 tcactttaga gacccacttt tagacatagt tgaaagtgat aaggatgtag atttacttgg
     6241 agtaatagta gtaggaactc cagatggaaa tgaaaataaa acctttgtgg gacaaagaac
     6301 ggccgcttgg ctggaagcca tgagagtaga tggagctata gtttcatcag atggttgggg
     6361 aaactcacat gttgactacg ctaacacatt tgaggaaata ggaaaaagag atatacctgt
     6421 agttggagtt acatttaatg ggactcaagc taagtttgtt gtaagcaatc aatatatgga
     6481 taccatagtt gatatgaaca aatcaaaaga aggtattgag acagaagtag ttggagaaaa
     6541 caacacaaat gaaatagatg ctaaaaaagc tttggcattt ttaaaactta aaatgagaaa
     6601 acatggataa aaaatttaag taagggaatg agttttgtgg gaatttctac atcaatgaaa
     6661 aacacatcac ttcatatatt tagagacccg ttactagatt tgatagataa tgattctgac
     6721 atagacttgt taggagtggt tgtagttggg acatcagagc acaatgaatg gaaaacattt
     6781 ttagcaacta gacttggaag atggattgaa tcattgcgac ctgatggagt gattataact
     6841 ttagatatgg caggaaacca gcatattgat tttacaaatg ctatagcaga gtttgtaaaa
     6901 tcagatatac ctacagtagg tcttactatt atgggagcag atggattagt aattaccaat
     6961 ccatatttgg ataaagctac cataattgat tataaaaaga caacaggcta catagaaaca
     7021 gaagtagttg gagacaatca catggatgaa gttgatgtta aaaaagcatt ggctttccta
     7081 aaattaaaaa tgcgtaaaga cgcaaaataa acctaagggg gaatttgata tgaaatttag
     7141 cagaagtata caagctatag actctcatac agcaggtgaa gcgactagaa tagttgtagg
     7201 tggaatacct aacataaaag gaaattctat gcctgagaaa aaagaatatt tagaggaaaa
     7261 tctagattat ttaagaactg ctataatgtt agagccaaga ggacataatg atatgtttgg
     7321 ttctgtaatg actcaacctt gttgcccaga tgctgacttt ggaataatct tcatggatgg
     7381 tggcggatac cttaacatgt gtggccatgg tacaatagga gctatgacag cagctataga
     7441 aacaggtgta gttccagcag tagagcctgt aactcatgta gttatggaag ctccagcagg
     7501 aataataaga ggagacgtta cagttgtaga tggaaaagct aaagaagtat cattcttaaa
     7561 cgtaccagct ttcttatata aagaaggtgt tgaagttgac ttaccaggtg ttggaactgt
     7621 taaatttgac atatcatttg gtggaagctt ctttgctata atacatgcaa gtcaattagg
     7681 tttaaaaatc gaacctcaaa atgctggtaa attaactgaa ctagctatga aacttagaga
     7741 tataataaat gagaaaatag aaatacaaca tccaacttta gctcatataa aaactgtaga
     7801 cttagttgaa atatatgatg agccaactca tccagaagct acttacaaaa atgtagttat
     7861 atttggtcaa ggtcaagttg acagatctcc atgtggaact ggaacaagtg ctaaattagc
     7921 tactttacat gctaaaggtg aattaaaagt aggagaaaaa ttcgtatatg aaagtatatt
     7981 aggaacttta ttcaaaggtg aaatagttga agaaactaaa gttgcagatt tcaatgctgt
     8041 agtacctaaa ataactggtt ctgcttatat aactggattt aatcattttg taatagatga
     8101 agaagaccca cttaaacatg gatttattct taaataagta ataaataaat taggaaatta
     8161 aaatagaata gtagccactt gtcggttgct attctatagc ttttaggcta taagattcag
     8221 aatatatgaa aaaataaaaa aataggagga tggattatgg tagcaatctt aaaggcatta
     8281 tatgtagtat tcgcagcaat ctttgcagtt gtgtttggta aagacgtagc taaagcaaac
     8341 aaagagggga aactagaaga acaaagtact ccaaaagttg ctgtaacagg atttataaca
     8401 gacttctttg atacattagg aatagggtca tttgctccaa caatagcaat gtcaaaggct
     8461 ttaaagttaa atataccaga taaaaaaatg cctggtacat taaacgtagc acatactatt
     8521 ccagttgtta cagaagcatt catatttaca tcaattatcc cagtagatgg tgttacttta
     8581 gtatctatgg ttgtggcagc agcagttggt tcatatatag gtgcaggaat aatagctaag
     8641 atggacgaga gaaaaataca acttgttatg ggtgtaactt tagcattaac agcaatatta
     8701 atgttattag gtcatccttg gattaacgta ttaccaggtg gaggaaatga attaggtctt
     8761 actggtgcta aattagtaat aggtgtaata ggtaacttta tactaggagc tttaatgaca
     8821 gctggtatag gtctatatgc tccaggtatg gcaatggttt atttcttagg aatgtctgca
     8881 aaagttgcat tcccaatagt tatgggttct tgtgcattat taatgccagt tgcaagtatg
     8941 aaattcataa aagaagatgc atatactaaa aaagcatcag ttataatagc tatatgtggt
     9001 ttaataggag ttttcgtagc tgcatatata gtaaaaactc taccaatgga tatattaaaa
     9061 gcattagtaa tagtagttat agcttatact tctgctacaa tgataatggc tgcaaataaa
     9121 aacaaaaaga ctgcttagtt aaagactact tagtgtttat taaatattac agctaataat
     9181 aaagattata aactgcttta aaatattgaa ttaatatttt agggcagttt tttatttact
     9241 aaattaatat aaagcaatta a
//
LOCUS       DAYATN010000140         9191 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.140, whole genome shotgun sequence.
ACCESSION   DAYATN010000140 DAYATN010000000
VERSION     DAYATN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.140"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            393..1598
                     /locus_tag="ACGI8A_003120"
     CDS             393..1598
                     /locus_tag="ACGI8A_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0181102.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1669..1890)
                     /locus_tag="ACGI8A_003121"
     CDS             complement(1669..1890)
                     /locus_tag="ACGI8A_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181103.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2031..2630)
                     /gene="recR"
                     /locus_tag="ACGI8A_003122"
     CDS             complement(2031..2630)
                     /gene="recR"
                     /locus_tag="ACGI8A_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFH0181104.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2643..2993)
                     /locus_tag="ACGI8A_003123"
     CDS             complement(2643..2993)
                     /locus_tag="ACGI8A_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFH0181105.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="ACGI8A_003124"
     CDS             complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="ACGI8A_003124"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFH0181106.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4766..5030)
                     /gene="ffs"
                     /locus_tag="ACGI8A_003125"
     ncRNA           complement(4766..5030)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGI8A_003125"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5068..5159)
                     /locus_tag="ACGI8A_003126"
     tRNA            complement(5068..5159)
                     /locus_tag="ACGI8A_003126"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5123..5125),aa:Ser,seq:gga)
     gene            complement(5160..5615)
                     /locus_tag="ACGI8A_003127"
     CDS             complement(5160..5615)
                     /locus_tag="ACGI8A_003127"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HFH0181107.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5936..7207)
                     /gene="serS"
                     /locus_tag="ACGI8A_003128"
     CDS             complement(5936..7207)
                     /gene="serS"
                     /locus_tag="ACGI8A_003128"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFH0181108.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7333..7538)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7666..8547)
                     /locus_tag="ACGI8A_003129"
     CDS             complement(7666..8547)
                     /locus_tag="ACGI8A_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFH0181109.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8559..9068)
                     /locus_tag="ACGI8A_003130"
     CDS             complement(8559..9068)
                     /locus_tag="ACGI8A_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFH0181110.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         2943 a         1598 c          973 g         3677 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata tcatctttat ctattctagt caatataatt
       61 ttttactttt tttatttttt ttatttatca tgtattcttt atatttaatt tttaaaactt
      121 aatgctaagt aaacctgttt atttcataaa actaatatat attctatgct ctattttcct
      181 ttttattcta tatttaaatt ttttatttac aagaatattt ttaatataac atatttgata
      241 aaattaattt acatttcttt cacttgtaat ttttgcaatc ttgtattaga ttaatattaa
      301 aaataattta tcaataacct tattcacaaa gacagttttt atatatcaaa atataatata
      361 aatactatgc tacattttag gaggatttta ttatgagcaa aaaagtatta ataatgtctg
      421 cttccactgg tggtgggcat aatagggctg ctctagctat taaagaagaa cttacttcca
      481 aaacacttga tggagagcca attgaatgtg aaataataga tagtttgaaa ttagttaata
      541 atacaatgga taaaataatt tcaagaggat acgaaaaatc tgctatatat actcccaaag
      601 cctatggaag tgtatataga ttatctgaaa caaatctttt atcaaaaaat gaatttaaag
      661 ataatctact aataacattc atggctaaaa aatttaaaaa acttattaga agcgaaaaac
      721 cagacttgat tataggaacg catccttttc caatgattgc tctaagtaca ttgaaaaaaa
      781 actttaattt acataataat gaatcaaatg cttacacaga gcatttttat aaacattata
      841 caaataccat taatgttcct ccgcttattt ctgtattaac cgattatact actcattcta
      901 catggataca aaatgaaatt gattactata ttgttggtca tgaatatgta aaagaattat
      961 tggtgtttga tggagttgaa ccgagtaaaa ttagaacatt tggaatccct gttgaaaaat
     1021 cttttctctc tcacagagat aaaggtattg ttctttctga gttgaattta tctcctgata
     1081 aattaactgt tttacttatg gggggtagct ttggggctgg aaatataaaa gagacacttg
     1141 atgaattact ggatactgat agagactttc aaatacttgt aattactggt aagaatgaaa
     1201 gcctaaaaga aaaaatcgaa aaaaaactta tgtctcgtta tcatgataaa aatgtatgtg
     1261 ttcttggata cactgataag atgaatgata ttttagcttc tatagatgta ttagtaagca
     1321 aacctggtgg acttactaca actgaggctc ttttaaaaga tgtacctatg atagttcctt
     1381 attatatacc aggtcaggaa gaagagaatc ttgatttctt atctaattgt ggtgcagcac
     1441 ttagaactac aaaaaaatat aacttgtcaa tattactaaa agttttaata gatgacccta
     1501 gcagattaga aatgctaaaa agaaatatta aatcaataag aaagtcaaat tcagcccaaa
     1561 atatagctaa tttaatttta aatataattt ctggttgata aaataaataa aatccctata
     1621 gtatatatat tatctattag ggatttttta ttttctaatt atttaatatt aaattggttc
     1681 ataaaagtct tcttcataat catactgata atttttataa ttatttctaa attcagcatt
     1741 tttatttcgt acattttttt tagttttttt cttattagaa aaacttatat cctcaaaatc
     1801 atcatattta ggtttatctt ttgatttcat ttttttaact cttttttctt tcattgcatt
     1861 atgttcttct aagttcataa ttggtttcat gttgcacccc tatcaaaatt tttaaatgat
     1921 ttatacaaca aattatacaa tacagaagaa atttattcaa taaatctaaa tcaaaattaa
     1981 tttgaattta actcaaaaaa ttacaaaaat cgaggtatta acctcgattt ttatatttct
     2041 cttctgcctt ctagtgcttt tgatattgtg acctcatctg catactctaa atcgcctcca
     2101 acaggaagac catgagctat ccgagtgact ttgattccca ttggttttac taacctagca
     2161 atatacatgg ctgtagcttc accttcaata gtaggatttg ttgccattat tatttctttg
     2221 acttcctgat tgccaagtct ttggataagt tcctttattt ttaacatatc tggtccaatt
     2281 ccatccattg gagaaattac tccatttaat acatggtact gtcctttaaa ctctctcgtt
     2341 ctctccatag ctgctacatc tcttggatct tccacaacac atataacact tgaatctcta
     2401 cttttatttg aacacatgct acatgggtct gtatctgtta tattacaaca aattgagcaa
     2461 tatcttattt ctctttttgc atctataatc gctttagata aagcttccac atcattagta
     2521 ttcatattaa taatatgaaa agccaatctt tgagcagtct ttcttcctac tccaggaagc
     2581 tttgaaaatt cctctataag tctcgttatg ggacctgtat aaacttgcat aaataccacc
     2641 tactagaata acccaggtat attcatacct ccagttacct tactcatttg acttgcttgt
     2701 atatcatcaa tatttctcaa tgcttgattt actgcactta aaactaaatc ttgtagcatt
     2761 tctatatcat ctggatcaac tacttctggc ttgatagtta tatctataac ttctttttta
     2821 ccattaactt ttacagtaac tgctcctcca ccaactgaag cttctacttc tttactttct
     2881 aactcttgtt gagctttttg catattttct tgcatttttt gagcctgctt taacaagttg
     2941 ttcatatttc caccaccagg catcattccg cctccaaaac cttttttagc cataaacact
     3001 attcctccta aacttatttt cattttatta ttatatcata atagtatttt attatttact
     3061 ctcattttca tcaatactat cttttatttc taatatctct tcactcacta tgttctttaa
     3121 tatttcctca ccaatgtcct ttttctcttt aacttcaaga tttatatctt taacttctga
     3181 tgtcagtact atttttacat taaaacttct atttaaaaca tctcttattg ttttttctaa
     3241 ataatttatt gtatcttgag agctcagtct agtttttgca aaatcaaatt tatcatcaaa
     3301 tataacatac aacatattag aatatacatt aaaacttttt acttcactta agagtgcgta
     3361 tattggcatt tttttatctt ttttaatttg tttaagtata ttcttccaag atgactctat
     3421 aagtataacg tcctcatttt ttacatcttc ataaactaca ttttcttgtt ttacatctat
     3481 ttctctatcg acctcatact ctttttcttt attattccca attttttcag acttaaagtt
     3541 ccccagttca atcatttttt ctaagttctc aactcttttt ataagagctt ctttactttc
     3601 atcaaacatt ggttgagcaa ttttcattat ggttatttcc actaaaaccc ttggatttga
     3661 agatgacttt atatcatcct gagttattga taatatattt aaaatcctta ttaaatcatt
     3721 tatatctata tttttagact gaattttaag ttgttctata gtttcttctg gtaatgaaat
     3781 tatttcatcc aattctgatg aaaccttaca taccattaaa tttctaaaat gatcaattaa
     3841 gtcatttata agatttctta tatctttgcc ccataagaca aattcattta atatctcaag
     3901 agacttttta gtgtcctgct ctactataca ttgagacaat tcaaataatt gttctatatt
     3961 tacgcttccc attaattcaa caacatcttt ataatctatc ttactttctc caaaagacat
     4021 acattggtct aatatactaa gtgcatctct aagagctcct tgagaatttc tagctattaa
     4081 attaagagct ttatcatcta tatcaattcc ttcatcttca caaatctttt tcattctaag
     4141 ggtcatatct ttaactgtaa ctcttttaaa atcgtatctt tggcatcttg ataaaatggt
     4201 tgcaggtatt ttgtgaggct ctgtagttgc taatataaaa attacatatg atggaggttc
     4261 ctctaaggtc tttaataatg cattaaaagc tccttgagac aacatatgaa cttcatctat
     4321 tatatatact ttatatttag cattggctgg agaatatttc acactttctc taagctctct
     4381 tatatcatct acactattat ttgaagctgc atctatttct acaacatcca ttgtattatc
     4441 ttttaataca gattcgcata tctcacactc attacacggt tcttcattta ttttattcaa
     4501 acagtttact gctcttgaaa aaatctttgc agtagatgtt ttaccagtac ctcttgttcc
     4561 acaaaacaaa taagcatgtc ctatattatc actatatatt tgattcttta aagtcttaat
     4621 aatatgatct tgaccaataa catcttcaaa tttttgtggt ctatatgctc tatataatgc
     4681 tttatgcatc tttttacctc tctttagcaa aattaataaa aaaatccctc attatctaag
     4741 ggattagtct tttatatatt aaatgctgta caccatctat cgacttaatt ccataggcgt
     4801 taccaaaaca gttagctcaa cttaggcacc cccacggcac acaaaagtga tcacttaccg
     4861 ctgcttcctt ccggatctga cggggttcat gagctttcat tgcgcaggac ctaagcatca
     4921 acaccactta tttagggcgg ccctacagaa aataagcctt tagatggaat tcaatcctgc
     4981 tatagcggat tgcaggttac agggcaccgc tacctcccca tctagtacag caaaattaca
     5041 accatattta attactttca ttgtttctgg cggagagagt gggatttgaa cccacgatac
     5101 agttttaccc atatactcgc tttccaggcg agcgccttcg accactcgac catctctcct
     5161 taatctcttt ttctcaatgc cttaaaaaat tcttgcaata tgatcgagca ttcttcttgc
     5221 aaaacatcaa aagctacttt tacattatta ttattaaaat aatctaattt aaactcatgc
     5281 tgtttttcta tatatgaatt ttttacatgt cttgtcccta ttataagctt ttttattcta
     5341 gaattaacta ttgcgccact acacattatg caaggttcca ttgtaacata taaatcacaa
     5401 tcagtcaacc tccaaccacc taatttttct gatgcttgtt ttatcgctaa aatttctgca
     5461 tgtgccgtag agtcttttaa cgtttctgta aggttatgag ctctagctat tatttgatta
     5521 tctttaacta taatagcacc tattggtgtt tcttttttat tatatgcttt atatgcttct
     5581 tttaatgctt ctttcatata aaatgaactt tccataattc ctcccaaaac ttataacaaa
     5641 cttatcttat cataattcat tttataattc aacttaattt tattttgttt atttaattat
     5701 tactatatat agctatattt ttttaaaatt agttctaaaa taatttttag atattcatac
     5761 aagagtgttt tataaaaata tttagtgttt tatgatgaaa attctgctgt gtaaattaac
     5821 ataaaaaaac tatgaaatta atatttataa taatttcata gtttttttat ttattgttta
     5881 ctcataatat attctcttca tcaaagcata ctctatatat acaattaata taaatttatt
     5941 ttataacatc aacacccata taaggtctta attcttctgg aactactaca gaaccatctg
     6001 cctgctgata attttctaat atagcagcca aacatcttcc tattgctaat ccagaaccat
     6061 ttaatgtatg aacatattct gcttttgatt ttttatctct tttaaatctt attcctgctc
     6121 ttcttgcttg gaaatcttca aaatttgaac aagaagatat ttctacatat ctattgtaac
     6181 ttggcatcca aacttctaaa tcatatttaa atgcagctgt aaatcccaaa tctcctgtac
     6241 atatttttac aactctatat ggaagaccta acatttgtaa catagtttca gcatcatgag
     6301 ttaatgattc taattcatta taagaatctt ctggtttaac aaatttaact aattcaactt
     6361 tattaaattg atgttgtcta actaaacctc ttgtatctct tcctgctgaa cctgcttcag
     6421 atctaaaaca aggagtatat gcacagtatt taattggaag ctctgcaaca tctaatattt
     6481 cattagcatg tatattagta acaggaactt cagctgttgg tattaagaaa tactctaatc
     6541 cttctatttt aaacatatct tcttcaaatt taggaagttg accagtccct ataaaactat
     6601 ttctattggc cataaatgga ggtaagactt ccgtatagcc atgcttagct gtatgagtat
     6661 ttaaaaagaa attcattaaa gatctttcta atctagctcc taatccccta tataaagtaa
     6721 accttgaccc agttattttc cctgctcttt caaaatctaa aatacctaga cctgtaccta
     6781 tatcccaatg cgctttatgc tcaaaatcaa atttagtagg ttctccccaa gttcttattt
     6841 gaacattgtc ttcatctgtt tcaccttgtg gtacctctgg atgaggaaca tttggtattc
     6901 tcattaatgt atattccatt tttgcttcta cttcttttac tttctcatca atacctttta
     6961 tcttatctga taactctttc aatcttgcct tttcttctgt tacatctttt ccttctttta
     7021 ttaattgagg tattttttta gaatttacat ttaactcatt tttcataact tcaacttctt
     7081 gaagtatttc tcttctttta ttatctaatt ccactacagc atcaaggtca aattctcttt
     7141 cacctcttct ttccatagct tttttgatat catctagatt ttctcttatt cttttaatat
     7201 ctaacatatt aattcctcct aataattttg ataatataag ttataattcc aacttaattt
     7261 acaaacatta tacttgtaaa ctcttagttt aaacaaattt taaaaataaa aaaagacctc
     7321 cgtttaccct ataaaaggga cgaaaatctt ttatccgcgt tgccacccta attgactata
     7381 ctaaaatgta taatcctctc agacctataa cgtaggtaaa cgtctagctt taactagaag
     7441 ctctagagta gattcaaaaa ccattgtaat gagttttcac catccactca ttctctgaat
     7501 acaatctgtt ttttactatt ttccttcaca gctttttaac taattgtttt atttgataat
     7561 cattatatta tatatattaa catctttcaa tactattatt caaatttttt tagaattttc
     7621 tttaattttt tatattaaat tcattttttc tatttaatta aaaccttatc ttttatccat
     7681 tgtatttaga ttacctgtat tattaaataa taatgaagaa ggaacaaatg gaacttttat
     7741 atcattctca tcaaatttat tttttacctt ttccattaca tcaaaatata catcccagta
     7801 atcatcagtt tttacccaag gtctagcaac tatttgcatg gtgttatttc ctatagagtt
     7861 taaacctatt ataggctttg gatcatttaa tattcttttt tcgtttgtaa aaatttcatt
     7921 taatatagac ttaactacat ctatatcact atcatatgaa atattatatg aaaaatctat
     7981 tcttcttttg ttttgatgtg tgtaattaat tatattatta cttgtcagtt gaaaattagg
     8041 tataattata gttttattat ctggcgtctt aagaacagtg ctaaatattt gtatgtcaga
     8101 aactgtacct tctacacctg aaccttgaat ataatcacct atattaaatg gcttaaaaaa
     8161 taaaataatc ataccagagc ctaaatttga taatatttct ttaaaagcta aacctatact
     8221 aaatccagca gctcctaaca cagctacaac tgatgtagtt tgtatcccta taatacctaa
     8281 gcttatcaat gatataatag ataacataga tatatatatt ccatatacaa taaaattatt
     8341 tacaccttta ctcattccat atttgattag aaactttgaa accatccttc ttactatttt
     8401 agatatataa atacctatca caaatacaat tattgcatat ataatatttg gtatattatc
     8461 actaaaatta tcgagcatat tttctaaagt tctcttagtt atattgctat ttgttgaggc
     8521 agtcttagcc aatgataaaa aattcattca actcctcctt atgataatat tccgttgtcc
     8581 tttaactctt ttactatagc ttctatagtt tcttctttta ttgcctcaat tgtatgaata
     8641 tggctacatt ctgtcagaac cgataattgt ttaaattcat ttgtctcaac tttcttcata
     8701 aagttgtcta tatccatttt agaataaata ttaagttctg cagttatctg accatatgtt
     8761 ggatgattta cgatgacatc ttttataata ccacctaaat ccacaattat ctttaattca
     8821 tcataaattt ctttagaatc cttatgattt ttacacttta tttgttttgt aaaatacggt
     8881 tgattatata ttatatatcc ttgaggtgta gctattattt caaatcctct agctcttata
     8941 agggctatat cttgaactat tacttgccta cttacattta atagttcagc tagtgttcca
     9001 ccttttacag gatgcttaga ttcttttaaa atatctatta atttacttct tctttcatta
     9061 gaattcatat tacaccttct ttccaaataa aaactttacc tatactatat aattttatat
     9121 tttatcttat tataaatcaa tattgacttt atattctatt taatcttaac ttttataata
     9181 aataaaaaag a
//
LOCUS       DAYATN010000141         9093 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.142, whole genome shotgun sequence.
ACCESSION   DAYATN010000141 DAYATN010000000
VERSION     DAYATN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.142"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            131..865
                     /locus_tag="ACGI8A_003131"
     CDS             131..865
                     /locus_tag="ACGI8A_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0181111.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDGYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(1008..1499)
                     /locus_tag="ACGI8A_003132"
     CDS             complement(1008..1499)
                     /locus_tag="ACGI8A_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFH0181112.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1848..2231
                     /locus_tag="ACGI8A_003133"
     CDS             1848..2231
                     /locus_tag="ACGI8A_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HFH0181113.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYNG"
     gene            2739..3122
                     /locus_tag="ACGI8A_003134"
     CDS             2739..3122
                     /locus_tag="ACGI8A_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFH0181114.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            3124..3474
                     /locus_tag="ACGI8A_003135"
     CDS             3124..3474
                     /locus_tag="ACGI8A_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181115.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(3575..4033)
                     /locus_tag="ACGI8A_003136"
     CDS             complement(3575..4033)
                     /locus_tag="ACGI8A_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181116.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLEEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(4078..4866)
                     /locus_tag="ACGI8A_003137"
     CDS             complement(4078..4866)
                     /locus_tag="ACGI8A_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0181117.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQEGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(5005..5442)
                     /locus_tag="ACGI8A_003138"
     CDS             complement(5005..5442)
                     /locus_tag="ACGI8A_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HFH0181118.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            complement(5676..5810)
                     /locus_tag="ACGI8A_003139"
     CDS             complement(5676..5810)
                     /locus_tag="ACGI8A_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181119.1"
                     /translation="MGILEGAAMIREIAIKIAKEKGITEQKAWPEAVKEFKEKYELVL
                     "
     gene            6264..6773
                     /locus_tag="ACGI8A_003140"
     CDS             6264..6773
                     /locus_tag="ACGI8A_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0181120.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            6800..6946
                     /locus_tag="ACGI8A_003141"
                     /pseudo
     CDS             6800..6946
                     /locus_tag="ACGI8A_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            7179..7715
                     /locus_tag="ACGI8A_003142"
     CDS             7179..7715
                     /locus_tag="ACGI8A_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFH0181121.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(8142..8942)
                     /locus_tag="ACGI8A_003143"
     CDS             complement(8142..8942)
                     /locus_tag="ACGI8A_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181122.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
BASE COUNT         3349 a         1241 c         1235 g         3268 t
ORIGIN      
        1 atattcattt atattattaa aaaatttgtt tatagaaact tttattattt tatataaaag
       61 tgataaaaac ttatattttg tatttaatag agtgttgcta agtgaatata tttaaaatgg
      121 aggaaacttt atgaaacaaa ataaatatga tgatgataga ttttttaata aatacagtaa
      181 aatggaacgt tctacaaatg gattagcagg tgctggagag tggcatgttt taaaaaaaat
      241 gttacctgat ttcaaaggaa aaagagtttt agatttagga tgtggatttg gatggcattg
      301 tcaatatgca gttgagaatg gagcaatttc tgctgttgga gttgatattt cagaaaaaat
      361 gttaaaagaa gctagaaaca aaacgcaact tgataatata aattacatat gtatgcctat
      421 tgaagatatt aattttccaa aagatagttt tgatgtagtt attagttcct tggcatttca
      481 ttatattcaa tcatttgaag atatatgtaa gaatataagt aattgtcttt caaataaagg
      541 taattttgta ttttctgttg aacatcctat atttactgca tatggaagtc aagagtggta
      601 ctgtgataaa gatggcacta atctacactg gccagtagat ggatattttg aagaaggagt
      661 acgtaagtca aattttctag gagaagaggt tataaaatat cataaaacat taacaactta
      721 tttaaatacg ttaatgaaaa caggatttga aatattagaa attattgaac ctcaaccaga
      781 agaaaaactg ttagatacaa ttcctgatat gaaagatgag ctacgtagac ctatgatgtt
      841 acttgtatct gcaaaaaaaa gataagtaaa tataaataat cattattgta gaaaaaaagt
      901 tgtagaaaat aattacacta atggagatga ttataccagt ggtattaatt acaccagtag
      961 taaggaggca aaagcattcg ttgcttttgc cattttttaa gttgcaatta tacttctacg
     1021 tagtatattt cgtttccatt ttcttctata agctttttca tatcttcaaa ttttaaaaat
     1081 acagtagcag tattatcatt tggatgtata cctattttat ccatttttaa taaatcttta
     1141 tcaaatacaa cttttacaac agcctcttta tcatttaata taccaaaagg agtcacagct
     1201 cctttagtaa gtttcaagta tttttgaagt ctttcttccg atgcaaaact aagaggttta
     1261 ctatcaattt gttgacgaat atcttttaaa tctgcttttt tgtctttacc taatacaact
     1321 aaaaaatgtt cttttccttt agcatcacga agaaataggt tttttacaac aaattccata
     1381 tgtaatgttt ttaattcttc catttcttca attgtataaa ctggtggatg atttacagcc
     1441 tcataaggta tgcttaactc atctaatttt ttatatactt cctgttgttg attgttcatg
     1501 atttcctccc attagaaatt tattttatat ataaataata ttatcacaga aaactttaaa
     1561 agaaaacgtt ttatataata tagacaatag attatatttt tataaagaag caagaatact
     1621 caataaaact taaagaaatt aaacaatttc taaaaatacc ttgaaaatag aaccttattt
     1681 gccatttata aattatataa aaagggatta tataaagtca tatatgaaaa aactatatta
     1741 tactatcttt tctttttgga ataaactata ctttaaatat agatatttaa tttaaataaa
     1801 ttttacagta ttaatagtaa attatcttag aaaggaatgt gctttatatg tctacagaca
     1861 aagttgttgg aatcatagat gaagaaacgg cagaacttgc aggaattgaa tatactggta
     1921 aaatttatgc ttcttctggt gttataaaac atataaagaa aaaacataga tgtcaactga
     1981 gtaaggatat ttttaatgat ataatagaca ctataaaaat ggtattaaag tcacctgaat
     2041 atataggaag tcatcctaaa aaacctggaa aaagtgtgga gtttataaaa aaaattgatg
     2101 attatatttt agtagctaca gaattagata caaaaaaagg atatttatat gtatcttcaa
     2161 tgtatgctat aaaagaagca aaattagaaa gtagagtaaa cagtggaaga gtaaaacaat
     2221 ataatggtta acataatatt attaattttc cctaaaatat tgaaaaatat tattatataa
     2281 gttataatat taatatacac gtagatattt attagttaga tttgaggttg gaaaaggcac
     2341 ccaatacgct actatagagt agttaataga gatgcaggat acgccgccct gccaaatcta
     2401 attaaattaa aaagagagga ttaattcctc tcttattgtt tttttgagat actaatttaa
     2461 tctataatag aaagattgaa ggtcgtttat acaaaaaaat tttcagttat atacattaaa
     2521 ctaattaaat aagatattta taatctatat gtgatacata ttatatatta ataaaagaaa
     2581 aatatataga aaatccttat aaaaatttaa attgtaatga ataggaagta attaaaaaga
     2641 atttttattg atttttaaaa atttagttag aaactatatc attatggtaa aaaatgtatt
     2701 ataatgttta gaaatattat aataaggagc gattaaacat gaatctatcg aagaaaatag
     2761 aagataaaag aaaagctaaa cagagagctg aaaaagttaa gaaagctaaa attgctacag
     2821 ctggtgtggt attaggagct gttactggtg cagtgagtgg agtacttttg gctcctaaat
     2881 ctggaaaaga aacaagagag gatataaaag atgcttcaca acaaattgct gaaaagataa
     2941 atatgaaaac tgtagatgta aaaggcaaag tatctgagaa gctagaggat aaaaaaggaa
     3001 actttataga atcaaagaaa aaaataaaaa aatatataga taacaaaaag agtgttgaaa
     3061 attctagtga ggaagataaa ataatagata tagttgaacc agttagtgtt actgaggaat
     3121 aatatggaca atacaataac gattaacata agtataatat atgtattatt agctatactt
     3181 gcttgtattg ctttagttta tttaattatt gtacttgtta aactacataa gattttaaat
     3241 aatgttggaa ctatgttaaa tgaaaatact caaaatataa atacaacttt aaataatctt
     3301 cctaatgttg tagaaacttt tggagaagta ggagaaaata tgaaagatgt aacagaagta
     3361 gttactgaag ttacagcaga ttttcttgtt actaaagaca gtgtcaaatc aaatatggat
     3421 gttatagttg atatattgac tataattaaa aacatttttc tagataaaaa ataataagtt
     3481 taattaaaat aagcctataa gatataattt aaacgacctt aaaatagcat ttataaggtc
     3541 gttttttatt agcttgattt gacactatgt gtttttaata attggtaata ttcctcttta
     3601 tcatttgaac atatatatct atgcttgttg tttcaattac atccataaga acatctttac
     3661 attcaagctc aaaatcttcc attccttcta aatattcatc atcttcatca aaaaagatat
     3721 aagaattata tagccattct tcaaattttt cttcaagtat atcccaatac gaactccaaa
     3781 ataatccaca acctgatata taagatgctc tactttgatt tatataaacc tcctctagaa
     3841 gcttttgtaa tctactgcta taaagaaaat ctgctcttcc actgcttgat attaattcat
     3901 ttaaatcttc tattgacatt aattcaactt taccactttt atcataagga gtataatttt
     3961 catcaaccca tttatgtggt ttgttttctc tgcatactgt tgctatcaac tctacaactt
     4021 ttgttcttat cattttttta tcctaactta tttatttttt ataaaataat catgatttta
     4081 taatttatct atttcttgta atgctaattt aacagtttca tatctatcag caccatttat
     4141 tactacatca ccttttacaa gtttattagc ttctccacct acaacataaa cctttttacc
     4201 taaaccttgc ttataactag acgcttcaat tattaaagaa tctttcaatt gccaatgtaa
     4261 aatctgagca gccactttat cagcatcacc tgtataaacg attacatttt catacacttt
     4321 attttcccct ccctcttgag aatttgatat agatttgttt agtatacctt ctgctatcaa
     4381 tttagcaact ttatctttat tattaacata ataatctgta tctgctttac tatctacaaa
     4441 acatacttct attaatattg ctggtgcaat agtatgatta agccaataaa gacctctaac
     4501 atcagacttt gcgcccctat ctttgaatac tgtagctaac ctagtattca ctctttctgc
     4561 ataggttttg ccattattag tcttatatat tgtttctgtc cctactggag ttgaagttgt
     4621 ggcatttgaa ttaaaatgaa tctgaacagc caaatcagtt tcctgtttat ttgctatagc
     4681 acattggtct gctaaatgat tacttgattc atcaactctt cctgtataaa cagtagctcc
     4741 acctatttta agccatttaa caattaagtc attcagtatt cttgtttctt tgctttcgtt
     4801 tatataccca acagctcctg taccttttcc aattaaagtg tgccctggta ttattactac
     4861 tttcaaacta acatctcctt tacaattaaa tttaacttct atttaatata tgcataacct
     4921 agaattttgt ttctttttat atcttatact tttattataa cccattttgt aaaaatatag
     4981 aatattaagt aaataatgtt aaaattataa ttttgtatat aagacattac ttgattgcaa
     5041 taattttata gcatattcat ctattctata tccagttcta taatatatat gtttaattcc
     5101 agcttgaatt atagccttag tacaatttaa gcaaggaaag tgagttacat aaatagagca
     5161 atttttaact gaaatacctt cttttgcaca ataataaagg gcattttgtt cagcgtgaat
     5221 ggttctaata caatgtccat ctctcatttc atgaccaact tcactacaat gcttatctcc
     5281 agaaatacta ccattatatc cagtagacac tattctgttt tctgaattga caattatagc
     5341 tcctacataa gctctatcac aagttcctct ttcagctact gtttcgcaca gtctcataaa
     5401 atattcttgc catgagcatc tattttcaag ttttttattc aaattatata cctccaaaac
     5461 ttatatctaa tatataagat ttatacctta aaaataatat taaaaacaaa tttatacgat
     5521 aaataaaatt tattaatact gtttttagtc taaattattt cattttaaat taaagttaca
     5581 aggaatctaa aattatatat atctttcttc gtaatattaa tgttacaata taaaaaactg
     5641 tctcaataaa atagagacag ttttacagtc aaagtttata atacaagttc atatttttct
     5701 ttaaattctt ttacagcttc gggccatgcc ttttgttctg taataccttt ttctttagct
     5761 attttaattg caatctctct tatcattgca gcgccttcta atattcccat ttttaatctc
     5821 ccttcaaata tctttttgta tagaatataa atagtgctaa gatttattac atgctgcata
     5881 tacaatatct atacaacaca taacttgtaa attcctttta ttttcatacg tctttaaccg
     5941 acatataatt atattcaata agttttaaaa ttatgaactc aattataatt atttttattt
     6001 ttttagaatc taaacactaa aaattgtagg taaatttgtc ttatgccttt ataaatattt
     6061 ctacagtttt aattactgga catatctatt tttattataa aaagcgatta aaaatcgaga
     6121 atatccgcat aaaaactgta tataagcaaa acggttaaag gaaaaagaag aaatttactt
     6181 gaatgttaaa atgcgttgct tgaagcaatg ggcagttttg cctataatat cggtaacaac
     6241 tgaaagaaag gtggttagcc ctaatgttaa gcgaaaacat taaaacaatc aggaagtcaa
     6301 aaggactttc acaacaagaa cttgctataa aactgaatgt agtgcgacaa acaatatcta
     6361 aatgggaaca aggactgtca gttcccgatt ctgatatgtt gatttccata tcagaggtgc
     6421 ttgaaacgcc cgtaagtact ttacttggag aaactattgt tgagtcggaa gttgataact
     6481 taaaagtgat ttctgaaaaa ttagagatta taaacttgca acttgcacaa agaaaaaaca
     6541 tgagaagaaa aatgtttcat tggttattta ttttattatg tgcagtcata ttaatagttt
     6601 ttgtggtctt agggggacta aatagtcctt acttggattg gaattatacc gaccctgaaa
     6661 ccgctgtttt tggagtagct tttcatgctt ttgagtggct atttgtcaga ttagtaccga
     6721 ttgtccttat aggagcaatt attggaatct ttttgacacg taagaaaata tgaagtaact
     6781 ctgcttttta taatacagca agcgttcctt aaaagcgtca ttctacatgg caatcgagga
     6841 cgactatgta aggaagaacc tttttgattt ccaactaagc gaagttattg aggacgacag
     6901 taagaccaaa accgcattaa gtaaagtaca ggaagcaaga ctgctggact tatactctca
     6961 tgcgtcagat gcaggaaatg cgaggtctgg tagcatgatt tacaacgatt tgtacaacga
     7021 ctgctagtag aaataatatg gagataagta ttatctacaa agtcagaaaa gaaatgactt
     7081 gataatttac tcatgttata attattgatg tgaaattaat aaatcgaaat tccttttttg
     7141 gatttaataa tactgagtaa ggaaaaagtg aattactttt gcaaggaggt gttaatatca
     7201 ggcaagtaaa gtcttttgtt tttccagtta tatccttaat attcttagac caaattagca
     7261 aagttcttat aggattattc ttaatggact ttgaaattga tataattggg aaatttttaa
     7321 gattcaatcc tgttcaaaat acaaatctct cttatggggg aaactttatt ggtattctat
     7381 ctaatttatg ggtattggta ttgtttaaca ttttagttat attagttatt atatctggat
     7441 atgcttttta taaatcaaaa aatgaacaaa caagctattc agtaaaagta attatgtctt
     7501 gtggacttgc tggtacaata tgtagcttga tagataaatt attttgggga ggaagtttag
     7561 attttttgca gataccaagc ttttttattt tcgacttaaa agactgctac cttactgttg
     7621 cagaaataat atttgttgtc ataggaattt tgcataatag agaaatatca atgaaagaat
     7681 acatatattt ttgctatcgt cagtttaaaa aataaattta gaagataaat ttaagtttca
     7741 aggtaaactt atttaatcac agcaaatagt tggcttactt aagcttttgg gctaaactgt
     7801 gagcactaat taagtctctg atatctaaca aacggagcac aaaaataaaa attaaatata
     7861 aagcacagtt aattttgatt aaaaagttga ctgtcttttc ttttgtagaa atttaagcat
     7921 aggtaataaa gaaatgactg catttaagca ttatgtatat aattggaggc aataaaaagc
     7981 cagcaaagcc cataaaatag agctgtgcta gcaacatgat gaattataag aagataatca
     8041 aaaacttacg ctttatttta atttaaaaaa atgattactg taaagaaatc attttttatt
     8101 ataatcactg atacatcaaa tattctaaat aaaattttta tttatctgga attgtatatt
     8161 ttttctataa tattaactac agcgtacatt gcccaagcac agatagccaa tacaaatact
     8221 cccatcatta ctaaatctaa tttaaatact tgagaaccat atactatcaa gtagcctatt
     8281 ccatatcttg aaactaaaaa ttctccaact attacaccta cccatgccat accaatatta
     8341 attttagtta aatttattaa atttcctgtg tttgcaggta atatgagttt aaataatatt
     8401 tgagatttag tagctccaaa actttttaac atcttaattt tttcttcatc tatattcata
     8461 aaataattat aagctgaaag tatagtaaca acaacagaaa tcgttacagc tgttacgacg
     8521 attccttcta ttccagcacc tacccaaact ataattatag gagctaaagc agtcttagga
     8581 agagcattta aaactaccaa aaatgggtct aatatttttg ataatttttc tgaccaccaa
     8641 agagcaattg ctaccaaaat acctaataca gtacctataa caagaccaag aacagtttca
     8701 tatacagata ttccaatatg tttaaataat tgaccactaa atgcatattg aataaataaa
     8761 ttgtaaatat cacttggttt gctaaataga aatgtattta taacatttaa gtcagctaaa
     8821 agttcccaga ttacaataaa accaattaaa attaatagct ggtagaataa tacatttctt
     8881 ttttctcttt ttacaccttt aacatagttt atatatcctt cagatctttt attaagcctc
     8941 atgtgtatct agctccttcc ataatatgtc gaaatacttt tgaaaatcag gaactttacg
     9001 tgttttgaga ggcgtttttg ttccatccat gttaagttca atatcgtaaa tattttttat
     9061 ggtagctggt cttttagata gtattgcaac ctt
//
LOCUS       DAYATN010000142         8939 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.143, whole genome shotgun sequence.
ACCESSION   DAYATN010000142 DAYATN010000000
VERSION     DAYATN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.143"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(70..678)
                     /locus_tag="ACGI8A_003144"
     CDS             complement(70..678)
                     /locus_tag="ACGI8A_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFH0181123.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(787..3525)
                     /locus_tag="ACGI8A_003145"
     CDS             complement(787..3525)
                     /locus_tag="ACGI8A_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0181124.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLKDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3673..4464)
                     /locus_tag="ACGI8A_003146"
     CDS             complement(3673..4464)
                     /locus_tag="ACGI8A_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0181125.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4500..5156)
                     /locus_tag="ACGI8A_003147"
     CDS             complement(4500..5156)
                     /locus_tag="ACGI8A_003147"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFH0181126.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5149..6114)
                     /locus_tag="ACGI8A_003148"
     CDS             complement(5149..6114)
                     /locus_tag="ACGI8A_003148"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFH0181127.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6220..6326)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6616..7560)
                     /locus_tag="ACGI8A_003149"
     CDS             complement(6616..7560)
                     /locus_tag="ACGI8A_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0181128.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            7671..8138
                     /locus_tag="ACGI8A_003150"
     CDS             7671..8138
                     /locus_tag="ACGI8A_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6194 family protein"
                     /protein_id="HFH0181129.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8308..8907)
                     /locus_tag="ACGI8A_003151"
     CDS             complement(8308..8907)
                     /locus_tag="ACGI8A_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181130.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2894 a         1487 c          874 g         3684 t
ORIGIN      
        1 ctataaaaaa ataagataca tacctataaa ataccttcct atagatacat atcttaccta
       61 tataaaaact tattctatat aaaaacgcca taaaaaatct tttgcttctt ctgcataatc
      121 tatatttata cgtttactag tttttatatc taactcatct ttactaaata ctttttttcc
      181 tttatcatca taataaaaat cacttatata taattcttca cccattatac tcttactatt
      241 tagacttcta tctattttta aagctttgca aagttttcct ggtccgttag taatattttt
      301 tacttgatat ttactcaact cagtgtaagc tttgttgtat ctattcatag atatctcatc
      361 taaagaagtt ataggctcta cccctcttat taatacacac tctggcacat tttctatatt
      421 tgcagataaa ttcaagcaat tatacatacc ataaattaaa taaacatata tatgtccacc
      481 atctaaatat aatggcttag ttctatctgt ctttttattg ccatatacat gtgctccttt
      541 atcatttaca cccatgtatg cttctgtctc tatgattttg gtaacaatta ctttattttc
      601 atatttcctt attagatatt tacctaatat tgatttggct aaatcaattc cattttttct
      661 aaaaaaatcc ttttccaaaa tttcacctca taaaatttat ttaaaacgca tcaaatacaa
      721 ctaaagtaat aaataaaatt aactcttatt aatgtgatat atataaacaa taaatttaga
      781 catttattat ttatatatat caaaaaattc tatctcaaac ttttcaatat tatcattgta
      841 atttgaatta tcatatactt cttcaacaac ctctttaatt gggaatataa cttttacatt
      901 agttccttct cctaccttac tatctatgta aatcttccca ttttgaattt caatcaatga
      961 ttttgtaagg cataaaccta taccacttcc aaattcattt gatataccac tgttttcaat
     1021 ttgtttaaat cgattaaata tatcctttaa tttttctttg tctatgccca ctccagtatc
     1081 ttttataatt atctctactg tttcgtcttt tttatatata cctacaagga tttctcctcc
     1141 ttctaaagta aattttatag catttgataa tatatttagt attattcttt ctatcgattc
     1201 tgggtcaagc cctaccataa cagtttcttc atctgtatca aaaattaaat ctattccttt
     1261 agacttagca taactactaa tagatgtaac tattctttct gttatgtcaa taatatcaaa
     1321 attaactgct ttaatcttat atatgtctga cttaacttga gaaatattta ttatattatc
     1381 tattaatctc aataatctaa acatattttg tttagttatt tttatatgtt cttttagtat
     1441 atcctgaaaa tcaattattt ttcctttttt ataaaggttt tcaataagtt gaactgaact
     1501 atatatatta tttaaaggtg tcttaagttc atgagatata ttagcataaa actccatctt
     1561 catttggtca tactgtcttt gttttattaa tgctttttta agtctttcag actctctttg
     1621 cacacttaaa tctttaataa ttctcactct acttcttttc tgtccaaagt ataatgttct
     1681 tgatatcatt tttacttctt taatgtgatt attttcattt actatatgga tactcttctc
     1741 tctttctcct atatttaaat tgtttatgta tttttttact aatatatttc tatatttttg
     1801 tgttgtaact cctaatacat cactagaaat tccgaacaac tctctaaagg ctctatttgt
     1861 atatagtact cttgatgtac ttaaatcctc aagaaaaata gcctctggta taacatccag
     1921 taatttttca tatattattt tttgttcatt tactgttcta agaatattta tctctttagt
     1981 tatatctcta aatgtaagca atttaaagag tttattatct gaccaatagt ctccaactct
     2041 aacttcttca acatgttctt caccttctga gtcagacatc acaaaattaa ctatacagtt
     2101 tgctgtgtca ttgtaactta ctatttcagc tttcttttct tgtgttagat ttaatctttt
     2161 aaattcccta ttgtcaaaga aatctatgtt aaaagtttca aaatattgtt ctaatgtcat
     2221 atcgactaaa tcacttgaaa cacatttaaa actcttttca tattttttat ttatacttat
     2281 taccttattc ttctttatgc caccttcagc acttcttaca agtataggag atggaatatt
     2341 tagtataatg ttttcatagt ctttatttat atttcttatt tcttcatcta ttttgatttc
     2401 atttgtaata tcatttaaac ttattaagta attgctttcg tctttattat tacttaaact
     2461 taacaaataa attttatcat ttaactctac cagtttatca attatactta tatcgttatc
     2521 aaatttcttc aatttttcag caactttttc atcttccttt ataaaagtta atattttata
     2581 ataattattc atatcctttg aaaattcatc tttaaattta ttatttgtgt aacttatatt
     2641 aaaatcatag tctgttacta tccatacatt tggtattgta tccagagctt gtctaagtaa
     2701 ttcttttctc tggtctatgt agttttttaa tgttctttgt ataatcatat ccttttctat
     2761 tgctaaattt atctttccta attgctctga ctttttactt aattttttat tgattgaatc
     2821 aagttcataa tatggataca ctactctatt attaagactg tatacatata cataataagt
     2881 atggttacat aatagtaaat atattactcc tattaatcta tctgaatcta ctatattaat
     2941 cttagaaatt gctacaaaac atataatagt tgtaaaaaaa acaaaatatg ttaaatcttt
     3001 tattaactta tagtgctcaa tgttactatc aagtttattc aacaaatata gtaaattaaa
     3061 catagatata caaatattaa ttaatataat aatcatattc cagttaatat cactaaaaat
     3121 atttcttata aagtaatcta atatcacaaa agcactataa aaaattagta ttgggcttat
     3181 tcttagtttt tttactttag aaatggttaa gtatataaaa gtagacatat taattatgct
     3241 taagttaagc aatacaattt ggaatttttg aatttttaaa tttactactc ctaaaatact
     3301 tacaaaaact aatacaagac accctacttt caatatattt ataaattcat ctgttttaaa
     3361 gctacttata taaactatag aatatccaaa tatccaaagt aaaattattc tatacatatt
     3421 tatagaaact gtatacaaat ttatatttaa aagtgcaata accaaaaata acccattagc
     3481 tagtatatag accttattct tacttaaagt tcctttagat tccatattcc ccaccacttc
     3541 ttcattttta tatagtttta cctataaata tgttaccaca attcttccaa aaaatttgtc
     3601 actttctagt tataaaaaaa aatgagtaaa tatttttaat tttactcatt ttgtagattt
     3661 tgtatattca gtttaaaaag ctggaactat acttccttta tattcatctt gtataaattt
     3721 tttaacttct ttactattca tagcctctga taaagcctta atcttatcac tatctttatt
     3781 attttctcta caagctatta tatttacata tggagaatca cttgattcta ttactattgc
     3841 atccttagta ggatttaagt tagctgttaa agcataatta gaatttatta ctgctccgtc
     3901 aacatctttt aatactgttg gtaattgctc tgcattcatt tcttttatta ctatattttt
     3961 aggattttta gttatatctt ttttagttat aagctctcca tcttttactt ctatcaattt
     4021 attttttgct aataatttaa gtgctctagc tccattagtt gcatcatttg gcacagctat
     4081 aacagctcca tcttttaatt catctaatga cttaactttt tctgaataga atcccattgg
     4141 ctctatatga actttagatg tataagtaag tttatatcct ttctccttca cagtttcctc
     4201 taaaaatgga atatgttgaa agaagttagc atccaaagac ccttcatcta aagctgtatt
     4261 tggaagtaca taatcatcaa atatttttac ttctaaatca tatccttttt cttttaacaa
     4321 aggttttgca acttccaata tctttgcatg tggatttgat gaagctccta ctagtatttt
     4381 tttatcctct ttgtttgaac aacctactgc tgatattgct attgctgaca ccaatgctac
     4441 tgataataat ttctttaatt tcatttttac tccccctctt taaaatgttt ataattaatt
     4501 tattttagtt tcttataagc cagatttcct actccctgga ttatctgaac taatattata
     4561 agtgctatta cagtatatac cattactgct gtatcaaatc tttgatatcc ataaataagt
     4621 gctatattac ctaatcctcc agctcctact gcacctgcca tagctgtata accaagtata
     4681 gatattattg ataatgtaat tccagatact attgaaggca tagcttcctt tatcataact
     4741 ttaaatacta tttgtctttt tgtagctcca aaagattttg ctgcttctat caatccttta
     4801 tcaacttcat ttaatgagct ttctataatt ctagcgataa acggtgctgc cccaatagtt
     4861 attggcacta ttgcagcagt ttcccctata cttgttccaa cgattaatct agttattgga
     4921 atcattgcaa ctataagtat cataaatggg aaacttctaa atatatttac tataaatcct
     4981 aatgcactat ttattgtgct atttggtttt aatccatctg gttttgtaac tactaatatg
     5041 attgctaata taaatcctaa tattgttgcc actatagttg gtactataac catatacaaa
     5101 gtttgtaaca atgcatttgg aaatagtgtt gttaaaaagt caattaaact attcatttat
     5161 catttcctcc catcttatgc ccttactagt tagatattgt tttacttgct ctccactttt
     5221 atctgaaatg tttataataa gtgaacctaa aatatcatct ttaaattgtt ctaatttccc
     5281 aaatattata gacacatcta tatttagttc tcttgccata gtcgtaataa tagcttcatt
     5341 agaaatatct ttagggaata gtatctttat atttgttcct tttggcaata ttatactttc
     5401 ttcacctatt aattttctaa gtcctttagt atttcttaag aatatttctt ctgtatctcc
     5461 tacctctaat acttctccat tttccattat tgctactctt ccacaaatct gctttataac
     5521 ttccatttga tgagttacaa ctattattgt tattcccaat tttttattta tatcttcaag
     5581 caatgaaaga attgactttg tagtatttgg gtctaatgct gaagtcgctt catcacaaag
     5641 aagtacttgt ggatttagtg ctaaagctct agcaattgca accctttgtt tttgtccgcc
     5701 acttagattt ctgggtttat catttttctt ttcagatatt ccaactactt ctaataactc
     5761 taaaactctt tttttaattt ctgcctttga gtatccaaaa cattctaatg gcaatgctac
     5821 attatcaaat acagtttttc tttcaagcaa tgaaaagtgt tgaaaaatca ttcctaactc
     5881 ttttcttaaa tctctcattt gcttttcatt caatgacttt acttctttat cagaaacaag
     5941 tacactgcct tcttggtatt cttctaatcc gtttatacat ctcagtagtg ttgatttccc
     6001 tgcaccacta tgacctatta ttccaaaaat ttctccagat tctatttcaa tacttacatc
     6061 tttaagcact tgaatctttc cataatactt attaacattt tttatgctaa tcataaaatt
     6121 tcctcccata tattctgctc agtttttatg tttatcctct cttatgtatt acaacaaaat
     6181 aaaagcccga gtataaactc aggcttagaa agctatttat actcatctat caactatatt
     6241 agttgcagga tttagcacca atgtatataa aatatactgg ttgccgggtt tcaaagggcc
     6301 agtccctcca ccactcttga taagacgtta tttaatttaa ttctattatc atttgttgct
     6361 atgttatata tgatatattc attaatagta tttgtcaata aatttattta aattttttat
     6421 tttttattat cgttaattaa ctatttttaa ttagttttct agctcctaac actttatatt
     6481 ttaatgtcta tataactata gaacatcatt ataagaaata aattaaataa catcaatcta
     6541 tctaaatcat ctgtaaagta aattaagaaa attaacattt tatccaaact ctatcctact
     6601 attaaataaa ataatttaaa tacaatgatg attatatagc tctagtactt tagactttat
     6661 caaatgttta agttctatag gttctattat tgtagcattt ttcccaaaag ttaaaaaata
     6721 ttcagccacc cagttaagtg tagattttgg tatatacata tctatgtatc cacctttaga
     6781 caagattttc aaccctcttg ccaacaaaaa ttctgtttca caacgtttta ctccctcatc
     6841 tgtcaaatta attttaatat gataatctgt acctacttcc atattcttaa gataatcatg
     6901 tatagaagat aaaaccttat atttcttact tgataagttt tctatactca aaatcttcac
     6961 aattctgtct acacgaaatt ccttaataga ttctgatttt atacagtaag ctggacaata
     7021 ccaaagtcca ttcatggcat acaaaccaat aggaacaatg gtacgtacac tatttttttg
     7081 ctttgatgaa tattcaattt taattgtaaa ttcattcatt actatattaa acaattcttt
     7141 caataatggt gttgaacagt gcctatctgg tgtccaaaaa acaaatttac gccttatatt
     7201 ctcaatatta tattgaatat catttggtat acaatttaaa aattttttta aaacagaaat
     7261 agtttcttgc tcaaatggca aatcattata atattctagt gactgatatg caaagaatat
     7321 agattttacc tcactttctg aaaatgcaat cggtggaagt atacgctctt taagtacatg
     7381 atatccacca gccgctccaa cttttgaata taatggaaat ccaatactct ccagcacctg
     7441 taaatctctt agtatagtac gttttgatac agaaaattct tctgctaatt ctcctgctgt
     7501 aaaacttctt tttgcattta ttgttatcat cagttcaatt aaacgctcag ctcttgacat
     7561 atttatactc ctaatttaaa tatagtgaca ataattgtca ctattatatg ataagatatt
     7621 cttgcatcta aagcaataat aatagtaaag aaagaaggga ttgatacttt atggaaaatg
     7681 atactattaa ggctgatgat attctcaatt attgtctatc aaacttagat gatgttgtac
     7741 taatggatag ttggggggaa cgagcaattt actacaatcc taatggtgtt ttaaagcgag
     7801 gggtatatgt tcttaccatt aaggaaaaag acagtaataa tgataaaggt tcgttagtta
     7861 gtcgtccaaa tgtataccgt gtgaatatag gattaaaaaa agaaactttt attgaaatgt
     7921 ttggatatat tccaaagcgt ccaggtgtag gtcaaatagt tgatatggat tttgatttta
     7981 caaaattgga cacaatcatg ccacatccta tctactcatg gatgggatgg atatgtgtcc
     8041 taagccctac tgaaaagaca tttgagaact ttaaaacttt aataggagaa tcttacaatt
     8101 ttgcaaaaca aaaatttaaa aagagaaaaa acaagtaaaa acgtgtgaaa ataacctcta
     8161 aataaattat aataaaaata ttaaggttac aagataaaaa ttaatatctt aataacatgc
     8221 aaaaaagcga agccaaaaaa tgtattattt ataaggcttc gctatatctc aaataattta
     8281 tttcaaattt caaaactatt tattctatta tttcttatta tttcctgtta ttcctaatct
     8341 atcattcata tcttttatca tttttctaaa accactacct atatcatttc caacatcttc
     8401 agttccatga actaatttta taaagtcatt ttgacctttt ttcatatctt catcagatac
     8461 cttatctaat ttttctattc tcttatctat atcttttact aatctttccc cttcagcgat
     8521 taggtcatca ttcattttct ttaactctgt atcttctgtt ttaccatctt ttttaagaga
     8581 tgttacaaat gattgtattt tatctctact atctttatat gcagccttta catcacttag
     8641 atatttttca tttcctggat attctttatc tttaaactga ttatttatac tttctggagt
     8701 atcataaata gaatatctat ttagacctgc tatataatca ttatagcttg ttgaataata
     8761 attcatatat ccttcattat tatttacaga ttcattagga ttttttcctg tattttcact
     8821 tggtttttca ttatttttag tacatgcagt tatacctata gctaatacta gtgctaatcc
     8881 tacaaataaa gttcttttta atttcatttt tattaccctc ctagatgttt tatactata
//
LOCUS       DAYATN010000143         8872 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.144, whole genome shotgun sequence.
ACCESSION   DAYATN010000143 DAYATN010000000
VERSION     DAYATN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.144"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            152..919
                     /locus_tag="ACGI8A_003152"
     CDS             152..919
                     /locus_tag="ACGI8A_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HFH0181131.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPYF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1137..1350
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1604..2638
                     /gene="argC"
                     /locus_tag="ACGI8A_003153"
     CDS             1604..2638
                     /gene="argC"
                     /locus_tag="ACGI8A_003153"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFH0181132.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2679..3896
                     /gene="argJ"
                     /locus_tag="ACGI8A_003154"
     CDS             2679..3896
                     /gene="argJ"
                     /locus_tag="ACGI8A_003154"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFH0181133.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3911..4771
                     /gene="argB"
                     /locus_tag="ACGI8A_003155"
     CDS             3911..4771
                     /gene="argB"
                     /locus_tag="ACGI8A_003155"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFH0181134.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKETE"
     gene            4801..5988
                     /locus_tag="ACGI8A_003156"
     CDS             4801..5988
                     /locus_tag="ACGI8A_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFH0181135.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVILKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6097..7119
                     /gene="argF"
                     /locus_tag="ACGI8A_003157"
     CDS             6097..7119
                     /gene="argF"
                     /locus_tag="ACGI8A_003157"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFH0181136.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7246..7863
                     /locus_tag="ACGI8A_003158"
     CDS             7246..7863
                     /locus_tag="ACGI8A_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HFH0181137.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(8083..8781)
                     /locus_tag="ACGI8A_003159"
     CDS             complement(8083..8781)
                     /locus_tag="ACGI8A_003159"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFH0181138.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT         3576 a          860 c         1585 g         2851 t
ORIGIN      
        1 atatatacaa tttaataatt cagataatct taaaataata ttatgtaaaa taaaaatata
       61 tatgacatga tgttgttgta ttcaaaatat taagataaaa atatatagaa tatatttaaa
      121 aattattatg caattagaaa aggagataag tatgcagatt attgatataa atataaataa
      181 tattgacaat gaacatatat gttgttcaat ttctgaaaaa aaaggagaaa attgtgtttt
      241 ttctaaaaaa gcttggatga aaaataattt tgataaaggt ctagtcttta aacgatttga
      301 tgtaagaggt aaagctttta tagaatatat tccagcagaa aatgcatggt gtcctattac
      361 tgcggacaat tatatattta ttaactgttt ttggatatct ggaaaattta agggtcaagg
      421 atattctaat ctattgctac aagagtgtgt tgatgatgca aaatcaaaag gtaaatatgg
      481 attagttgtg ctttcgtcta gtaaaaaaat gcctttttta tctgatgcta aatatctaaa
      541 atataaggac ttttgtttag cagatacatc agaaccaaat tatgagctat tatattttcc
      601 ttttactgat aatgctccta ttccatattt taagaattgt gtaaaaaaaa gaaaattaaa
      661 tgagaacggt atagttttat attacagtaa tcagtgtcca catacggaaa aatatgctcc
      721 tattttgaaa aaaattgcaa attctagagg tattgatgta aatcttataa aattcgaaac
      781 agcaaaagaa gcacaaaatg ctcctgcacc atttactaca tacagtcttt tttttaatgg
      841 tgaatttgta accaacgaga tactttctga gaaaaagatt gagaaatttt tagataccaa
      901 cattaaaaaa tttaactaaa aagagatgta aatattgtat tttgaaaata tattatttta
      961 taatttaata tattttggat gttttttaaa ttgacgaatg aaatataaaa attatatcgc
     1021 aatttagata caaaatgctg gaaattaacc atatagaaaa tatttccaaa aatttaaaaa
     1081 ttatattgac aaagttaaaa aaataaacta tcatataaaa caataagaaa ataaattgaa
     1141 atcctgtgaa aaagagagta gcataaagct agttgataga gagctgggaa tggtggaagc
     1201 ctagtaacga attttattgt gaatagagct ttggaggaga ttaccagaag taatagtatg
     1261 gtaattggtg aatctcgcct taagagatta gagtgggtaa gattatctta tcaattagag
     1321 tggtaacgcg ggtgtatatt cgtctcttgg tctttgacca agaggctttt ttaattttaa
     1381 atgtacattt tgtagatact ttttaatttg agttttaatg attattgtaa ttttaccaat
     1441 aagagtggta ccgcggaaaa tcgcctctta gtatttataa tgctaagggg ttttttgttt
     1501 taattagagt attttaatct acgtattatc aaatgattat catatagtaa agtggaaaga
     1561 taggatttta agactatttg aatataaaaa tggagggaac aaaatgaata aaataaaagt
     1621 tggtattatt ggtgcaactg gatatgtcgg ggcagagttg attagattat taatgaatca
     1681 tgataaagtc gaagtgacag cgataggttc aaattcgtat gttggaaaag atatagttga
     1741 tatatatcca agtataggat ataaaaataa catgatatgt atagaaaatg aaaaagttat
     1801 agatatgtgt gatgttgtat ttacagccct tccacatgga gttagtgaaa agtttgtaat
     1861 aaaggcaatt aaatcgaaaa agaaagttat agatttaggt gctgacttta gaataaaaga
     1921 tgaagaagta tatagtaaat ggtatggagt atcttttata gataaaatct tacataaaaa
     1981 agctgtatat ggattaagtg aaatatacaa agaagatata aaagatgcag atataattgc
     2041 aaatcctgga tgttatccaa ctagtatttc tttaccattg atgccattac taagttctaa
     2101 attgataaaa aataataata taataattga ttctaaatca ggtcttacag gtgctggtag
     2161 agaattatcg attagtagtc actttacgga ggttaatgaa aatatcactg catataaaat
     2221 agggaagcac agacatactc cagaaataga acaaaattta agtgaatcct gcaaagaaaa
     2281 agtaagtgta gtttttacac caaacttaat cccagtaaat aggggaatct tatcaactat
     2341 atattgtaca aaagaagata atatttctat aaatgatata cataggaaat taactgatta
     2401 ttatgaattt aaagaattta tagaaattct tccattagat aaggtagctt ctttaaagaa
     2461 tgttagattc tcaaataaat gtgtaatatc attacatgaa aatggagata cattgataat
     2521 ctgttcagct atagataata tgattaaggg agcagcaggt caagctatac aaaacatgaa
     2581 tataatattt ggtattgaag aaaatacagg tttaaaaaat attgcaccat cattttagaa
     2641 ataaaaaata ctaaactcat ataattagga ggataaatat ggagattata aagggtggag
     2701 ttacagtttc agaaggattt tttgcttcag ggattcactg tggcttgaga aaaaataaag
     2761 aaaaaagaga tttagctttg gtttattcag atgttttatg tgatgcagca gcagtagtaa
     2821 ctcaaaataa agtaaagggc aatcctgttt atgttacaca ggaacacatt aaaaatggaa
     2881 aagcacaagc tatcatagta aatagtgcta atgctaatac ctttaatgga aaagaaggac
     2941 ttataaatgc ttataaaatg gctaaattca ccagtgataa attaaatatt aaagaaagtg
     3001 atgttttggt tgcatctaca ggtgttatag gaaagccatt aaatatagat ttaatagaag
     3061 aaaatatgga tgaattagtg aataatctaa gtaaacaagg tcatattgga gcaagagaag
     3121 ctattatgac aactgatatt ataaaaaaag aaatagcagt agcaataatg tatggagata
     3181 aaaaaataac tattggaggt atggcaaaag gttctggcat gatacatcct aatatggcaa
     3241 caacgttagg atttataact acagatgcaa atattgatgg agttttgcta aaagaagctt
     3301 taaaaattgc tgttgacaaa agttttaata gagttagtgt agatggagat acaagtacta
     3361 atgatatggt aatgattcta gcaaatggaa aagcaaaaaa tgatagaata aacaaaaaag
     3421 atgaacatta tcaagtattt ttgagtgcac ttacatatgt ctgtatagaa cttgctaaac
     3481 ttgttgcaaa agatggagaa ggatgtacaa aactaattga atgctatata aatggagcat
     3541 taagtgaaga acatgcagta aaattagcaa aaacagttat atcttcttca ttagtaaaga
     3601 cagctgtatt tggagctgac gcaaattggg gtagaatact gtgtgcttta ggttatgcag
     3661 gtgaagaaat agatatggaa aaagttgata ttatttttga gagtatgaaa ggatatattg
     3721 aagtatgtaa aaatggaaat ggattagatt ttaatgagga aaaagcaaaa aaaatccttg
     3781 aagatgacga aataagtata ttagtagatt taaatatggg caatgccaga ggaaatgcat
     3841 ggggatgtga tttaagctat gattatgtaa gaataaatgg aagttataga aattaggagg
     3901 atatacttta atgattaata tagaaaaagc aaatacactt atagaagctt taccatatat
     3961 agaaaaacat cagggaaaga ctattgtagt taagtatggt ggaagtgcaa tgaaaaaaga
     4021 tggtctaaaa gaatctgtta tggaagacct tgtgttaatg agttatgttg gaatcaatat
     4081 agttttagta catggtggag gggcagaaat caataagatg ctagcaaaag tggatataga
     4141 aagtaaattt gtaaatggtc ttagatatac agatgaagag accatggaaa ttgtaaaaat
     4201 ggtacttgct ggaaaggtta ataaagactt agtaaataaa atacatacaa aaggtggaaa
     4261 agcagtaggg ctttgtggaa tagataataa tatgatttta tgtgacccat ataaaaatta
     4321 tgaacttgga tttgtaggag aaatcaaaaa agtaaatgtt gagcttatag aatcttgtct
     4381 aaagtcaggt tatatttcag taattgcaac tataggtgtt ggagatgatg gagagactta
     4441 taatataaat ggtgacacag cagcttctgc tatagctaaa gagttaaatg cagataaatt
     4501 gatacttctt acagatgtcc ctggtttgtt aagagagcca gatgaagaaa aatcattaat
     4561 aacagaagtt atattagagg atgttgataa attatttgaa gaaggtataa ttactggagg
     4621 tatgatacct aaaatagagg gatgcgtaga tgccttaaat aacggagtaa atagggtaca
     4681 tattttggat ggaagagttc cacattctat aataacagag ctatttactg atagtggaat
     4741 aggtacatta ataagaaaag aaactgaata aaattataag tttaagaggg gagaataaga
     4801 atgaataacg acaatacaat ttcaaaatgg aatgaatatt ttatagatac atataaccaa
     4861 cccaattttg tcatagatta tggtgaagga agttgttttt ttgatactaa tggcaataaa
     4921 tatatagatt ttacaagtgg atatggagta tcttctcttg ggtatagtaa tagtaattta
     4981 aagaatgctc taaaagagca agtagataaa ttattacata cttcaaattt atattttaat
     5041 gagccagtgt tgtattcagg agagaaaata attaattcaa gtggaatggc taaagtatat
     5101 ttttgtaatt ctggaacaga agcaaatgaa acagcattca aaatagcaag aaagtatagt
     5161 agtgacaaat atggtaatgg tagaggaact atcataagtt taaaagactc ttttcatggt
     5221 agaacaatga tgtctttaat ggcaactggt atggacaaat accataagta tttttatcct
     5281 cttcccgaag gttttaaata tatagaaaga aataatattg aagatttaaa aaataatctt
     5341 gattccacag tttgtgcaat aattcttgaa gcgattcaag gcgaaggtgg agttaatgtt
     5401 cttgaaaaag attatgtttt ggaaatagta aaaatatgtc aagaaaagga tattgtagtg
     5461 atttttgatg aagtccaatg cggtatagga agaactggta aactttttgg ctatgaatat
     5521 tttgatgtaa aaccagatat agttaccgta gcaaaagggt tgggagcagg aataccagta
     5581 ggaggagtat tggtaaataa aaaactttca aaagtactag gaaaaggaga ccaaggtaca
     5641 acttttggag gtaatttatt agctatggtg gcagcttcag ttgtattaga tgagatatct
     5701 aaagatggct tttataatga agtattagaa aaaggaaatt atataagaaa atctattgaa
     5761 agttttaata acaaagttat attaaagaca aaaggtattg gtctaatgat aggaattgaa
     5821 actaatattg aatctagtat tatagaagag aaagctagaa aaaaaggatt gcttattttg
     5881 actgctggaa aaaatgtatt gagattttta ccaccattaa caatcagtta taaggaaata
     5941 gatgaggctc ttgaaatcct aaaagatata ttactagaaa ttaactaaat attcaaaaaa
     6001 tagttgatta aagataaaaa ttagaagtaa ataaaaaagt aaagtgctaa caagtttaat
     6061 tgaatttaaa aagtttataa gaaggggcga attaatatga ataatataag tttaaaaggt
     6121 aagagttttt tgacattaaa ggattttaca aaagaagaga ttagatattt attaacttta
     6181 tcaagttatt taaaaactaa gaaaaaagtt ggtataaaag gcgatttgct acaaggaaaa
     6241 aatatagctt tattatttga gaaaacatca acaagaacta gatgttcatt tgaagtagca
     6301 gctcatgatg aaggagcaca tgtaacttat ctaggtccaa atgattgtca tatgggtaaa
     6361 aaagagtctg tatcagatac agcaaaagtt ctaggaagat tctatgatgg aatagaattt
     6421 agaggcttta aacaagaaac agttgaaagt ctagcaaaat attctggtgt acctgtttgg
     6481 aatggactta cagatgaata tcatccaaca caaatactgg cagatttttt aacagtaatt
     6541 gaaaatgtag ataaagattt gaataaagtt aaatttgtat atgttggtga tgctagaaat
     6601 aatatgggga actcactgat gataggatgt gctaaaatgg gaatacattt cgtagcacta
     6661 gcacctaagg aactttggcc aaatgaagaa ttggttaagg aaatgaagga aatagccaag
     6721 aaatccaaag gagatataat gttgactgaa aatattgatg atgttaaagg tgcagatgtt
     6781 atatatacag atgtatgggt ttctatggga gaagaggagc aatatgaagc aagaataaat
     6841 caacttaaag attatcaagt aaatatggat atgataaaga aaactgaaaa tgaaaatgtt
     6901 atatttttac actgtcttcc agcatttcat gatttagaaa ctaaggttgc tcaggagatt
     6961 tatgaaaagt tcggattatc agaattagag gttacaaacg aggtatttaa ttcaaaatat
     7021 tcaaaggtat ttgaagaagc tgaaaataga atgcatacaa taaaagctgt tatggtttct
     7081 actatgactg atgtaaatat gaacaataat ttaatataga tatgttttaa tatagacttt
     7141 actttaatga aaatttataa aatattttaa atatattatt caaaaactct cattatatgt
     7201 taaaataact tatgtatcaa caaattaaga gggaggattt gagatatgaa aaaaatcata
     7261 ttagctttaa caactttttt attagcaata tctttaacag catgttcaag tgcaaaacca
     7321 gaagatacaa ttgatagttt ttttaacagt gcgaaaaagt ttgactttga aggcatgaat
     7381 aaggtaatgg aaaataatga cgaaaaatat aaggatatat taaaggaatt agatacaaaa
     7441 gacccaaatg cccaatatgt tttagattat ctaaaacaga atgcatctaa aatcacatat
     7501 acaataaaag acagcgaggt taaaggtgat aaggctacaa tcaaagtaga atgtaaattt
     7561 atagattcaa caccattact tcaagaaata gtagctgagg catttacaaa gatgattggt
     7621 atgagttttt caggacaaga cttaactgat gagaaaacaa ctgaaatgtt agtatcaatt
     7681 atgaaagaaa aacaaaaaag cgttaaagag acttatgtta caaaaaatgt agagtttgaa
     7741 tgttctaaaa aggataataa atggataata agttcagtaa atgatgcagt agctgatgta
     7801 ttgttatcta atcttgttac agcaggacaa gaattttcta actctatgaa tacacttgaa
     7861 taatattgtt taggtgtagt aaagtaataa atatatgatt atttttataa ataaacatat
     7921 aaatatttat gaaggatacc atttttggta tcctttttat taatataatg aaatacatgg
     7981 ttaatttcta tattgtcaag aaactatgat atatgttata agtctatact atttaaaata
     8041 aacttataac atatattttt tataatatag tgtatgaaaa atttatttaa ctaattttag
     8101 atatttattg acaactgatt cagccaagca ttttgatgaa tcaacaatat ttattgaagg
     8161 atatgatttt tctaaaacta catttaaatc agtacaagca attactatat tttctataga
     8221 ttcattttta acatcttcaa taagtttatt ccaaatatca atattgtgtg gattttcttt
     8281 atccattttt atattttgaa taagattgtt taattttatt tgccattcat ctttaaatat
     8341 aaattcatgt ccattatgta ttataccatt ctggtaaatt gtagactcaa aagttgaact
     8401 agtcgaaaaa agagttactt tttgtgagat agtaggtaat tttttaactg tttcttctac
     8461 aatatttaat attggtatat taatagactc ttttaattct ttaaagtata tatgtgctga
     8521 attgcatggc attgcaataa aatttacacc agttgattca agcttttgta atccatcaat
     8581 tatagtttct ttcataaggt catgatttat tggacggtca atataaaatg gggtaggtaa
     8641 tgaatatatc atcatctttg gaaattcatc atcatatttt gctccatatt gagattgaca
     8701 ctcatcaata attaaatcaa caaaaggggc tgttgacctt ggacccattc cagccaaaat
     8761 accaattaat ttttcattca taaaaattat ctcctttata tataaatata tttatgtata
     8821 aaataaaaat ctataaaagc ataaaattat attttataat agattatatt gt
//
LOCUS       DAYATN010000144         8872 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.145, whole genome shotgun sequence.
ACCESSION   DAYATN010000144 DAYATN010000000
VERSION     DAYATN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.145"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(35..1540)
                     /locus_tag="ACGI8A_003160"
     CDS             complement(35..1540)
                     /locus_tag="ACGI8A_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFH0181139.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLINLNKELDTLEKQKE
                     RLHDFLEQGIYDSNTFGERLKILLSRIENAREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
     gene            complement(1739..2593)
                     /locus_tag="ACGI8A_003161"
     CDS             complement(1739..2593)
                     /locus_tag="ACGI8A_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0181140.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            2978..3259
                     /locus_tag="ACGI8A_003162"
     CDS             2978..3259
                     /locus_tag="ACGI8A_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181141.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            3409..4257
                     /locus_tag="ACGI8A_003163"
     CDS             3409..4257
                     /locus_tag="ACGI8A_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFH0181142.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            4309..4830
                     /locus_tag="ACGI8A_003164"
     CDS             4309..4830
                     /locus_tag="ACGI8A_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFH0181143.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
     gene            5199..5714
                     /locus_tag="ACGI8A_003165"
     CDS             5199..5714
                     /locus_tag="ACGI8A_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFH0181144.1"
                     /translation="MNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVDNKMTAQ
                     DLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDKEISNQE
                     LSDIENKMFIKEALTKLPELEFEVIILRYYHDLRFKDIAKIMESSVSVTKYRMSRALK
                     KLELYLEGGKL"
     gene            5711..6472
                     /locus_tag="ACGI8A_003166"
     CDS             5711..6472
                     /locus_tag="ACGI8A_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181145.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICIFLFSTSADSNSFYNSLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            6510..7376
                     /locus_tag="ACGI8A_003167"
     CDS             6510..7376
                     /locus_tag="ACGI8A_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181146.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSIEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            7397..8638
                     /locus_tag="ACGI8A_003168"
     CDS             7397..8638
                     /locus_tag="ACGI8A_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFH0181147.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNITTDEGGFSNF
                     KPDIYWKFFYPNKAFYNFIIESYSNPNDYNPEIIKSMPLEDGVNFYKFRNEKVNTILN
                     MEYPDKNYTQKEKDFWLSKNKDIEEPYLNGYYQGWNYFLLARDLLIFTIVAVCISTAS
                     VFSGEYQSGADSIILSSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGID
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFLKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
BASE COUNT         3338 a         1085 c         1216 g         3233 t
ORIGIN      
        1 taatcagtct atttataaca tttaaaacat ataattaatc tcttgggata ttagggtaaa
       61 gtattaaaga aaaatcatca gatttttgat taggttcttt tttatagact gcgtaattta
      121 ttacactttt aagcaatata ttttttagat aaggttcttt ttcctctgta tattcactta
      181 aaattttttc aagttgagga acaatttgtg ttttaactgt atttctttcc ttttgcattt
      241 caatatctat atttgtttta ttaatagctt ctcttgcatt ttctatacga gataagagta
      301 tttttaaacg ttccccaaat gtattactgt cataaattcc ttgttctaaa aaatcatgta
      361 gtcgttcttt ttgtttttct aaagtatcca attctttatt taaatttatc aaggtatgtt
      421 ctaaagtatt ttcaggttta gagttgtctt tttcatcttt taaagatagt ctataattta
      481 ctaaccattt atttaagacc tctaatattt tattttctaa tatgtatagt ttagtagctt
      541 tatttttaca attagtacaa accatatatt tctcatatcc atttgatgta gtattagatt
      601 tggctattaa agtatgatta cagtatccac attttacaat tccagccaat ggatttgtta
      661 ttttattgtt tggagtaggg gaattaatac gtttttttct tatattttgt gccttattcc
      721 atatgtcttc acttattatt ggttcatgct ttccttttac ctcaatttgt tcctcaattc
      781 ctcttgttct actagaattt cttttcctct cgactttatt ccatactata tatccacaat
      841 acactttgtt ttttataata tctcttatac ccttttcata ccaagttcta cctgttgtgg
      901 tcttataacc aagttcgttt aaataattgg atattttaca tgcaccatct ccttttaaat
      961 acatatcaaa gattatttta acaacagttg ctttatcttc atttattaca agtactcttt
     1021 ctttattatt taaatattta atatcatacc cataaggagg agcttgagct ataaatttac
     1081 cctcttcaat acttttgatt tttcctcttt gcatacgtct agatattaat tttaattcct
     1141 tacgagccat aaaagcttca aattcagaat actcctcatc aaactcatca tttaaatcat
     1201 acgtttttct aggagtaata attttcgttt tagatttttt aaatgtatct agtattaacc
     1261 cttggtcttg catattacca cgacccaaac ggtcaatatc catcactaaa acagcatcat
     1321 agttattatt tttaatttca cctagtaatt ctagcatctt aggtctatat tctatacttt
     1381 ccccagaaac aagttcttct ttaatttcaa tgacatttaa attttgttct cttgctagtt
     1441 ttaaaagagt tgtcttatgt ctacttagag tttcaaattc acctctatgt tctgcttctt
     1501 catcagctct gcttttgcgt aaataaattg ccacattcat taaatcacac cccttgaata
     1561 tattatattc agtaacagat tgattatatt taaaacacaa aaataaacct tcaaaataca
     1621 agatatattt tttaggttta tttttataca gtaaatttta tagtatattt aaaagcttgt
     1681 aatctaagta aatatttata ctattaattt tatgctttct tcatttttta attttaaact
     1741 atttttttac tacaggtatc catacttcaa atttagcatt ttgagggtct ggatttaagt
     1801 acagttcaat atcaggacca ttatcatatt cataaccaga agtaggtaac cattctgtta
     1861 caatacgctt ttctagttct tgtattgctt gtggacattg tccttctcca gaaaatattg
     1921 cccaagtaaa agctggaaca gtgtattctt ctaatgtatt atctatagat tttgtacttg
     1981 aaacagatat aaaatacttc cattcttctg aattattaca aatacttata cctaatacac
     2041 cttgaagttg actatccatc atagggatta atttttgaag cgttccatct aaagcaacct
     2101 tttgccacat ttggggaaca atttcaaaat tcttttctag ttcattatga agtggctgag
     2161 atacaccaac tatacgaaat gcttctttct tttcaattct ataattcatt tcttcggctc
     2221 ctttaatact aattttaaaa ctgattggag gaaaggcttt taaagttgtt atttctcttt
     2281 tttttataac tgatggggca acaccatgaa tacttttaaa tgcacgattg aatgctgttg
     2341 gagaagagta tccatatttt aaagccacat caattatctt tttatcatca ttttgtaatt
     2401 ctacagctgc taaagacatc cgtctacgac gaatatattc agaaagtggt acatctgcca
     2461 tataagcaaa cattctttga aaatgatatg tagaacaaca agctattttt gccacttgtt
     2521 catactcaat ttcttttgtt atgttttctt caatatagtt aattgctttg tttaatcttt
     2581 caatccattc cataaaatca gctccttgta atttatataa tatctatttt aaaatagtta
     2641 atcctctctt tctatgcact aattaactag cttaataaag aatgaacatg agaaagtctg
     2701 taattaacta caaaaatgaa caaatagtat tgtttttcac attaaagtat atcacaaatt
     2761 agtatctaat ttttatataa gataacagtt ttaatttatt atgaaatttt tataatattt
     2821 tagcttagac atttaattat atataacatt ttatttatag ttaactattt ttgaagttta
     2881 cccttaatca agcaacactt agttattcaa ttataaataa tgattgagaa taggacaatt
     2941 tttaagatac ataaaatata aagaggaggg gatttaaatg ccaaacatag aaaaagactt
     3001 agatggtaca actactataa catacctcaa cgacgatata ggtgttccaa cagacgagaa
     3061 tggatataaa atatctatga caatactaca tggaaagaca atctgccatg tagtatctcc
     3121 tgaaacaatc ttaggaagaa agttcacatt agaagaaaaa caagaaaaag atacaataat
     3181 atatggagca atagaaaaaa taatggtaga cagacaatta caaataaaaa attcaaacaa
     3241 agaagttgct agtcattagc acttctttaa taagtaaagt aggacaagta ttttttataa
     3301 tattattgac ttatagtcta attagactat aatataaaca tagatagtct aattagacta
     3361 ctattaaaaa taaatacaat aagaatttaa gaaaggataa taataataat gattaaatca
     3421 tttttaatgt taggacagtc caatatggct ggaagaggat tcataagtga agtgactcca
     3481 atttataatg aaagaataca gatgttacgt aatggaagat ggcaaatgat gacagaacct
     3541 attaattatg acagaccagt ttctggggta agtcttgctg catcatttgc agatgcatgg
     3601 tgctgtgaaa atcaagagga tagaattggg ctaattcctt gtgctgaggg aggcagttcc
     3661 ttagatgaat ggaatataga tggtatactt tttaaacatg ctataagtga agctaaattt
     3721 gccatacaaa gcagtgaatt gacaggaatt ttatggcatc agggagaaaa tgacagtaat
     3781 aatggcaact ataaatttta ttataaaaaa ttactttcta ttattgaaac tcttagaaaa
     3841 gaattaaata ttccagatat accaattatt attggtggat tgggagactt cttaggaaaa
     3901 gtaggttttg gaaaaagttg tactgagtat gtatttatca atcaagagtt acagaaattt
     3961 gcttttgaac aagataattg ttactttgtt acagcaacag gtcttacatc taatcctgat
     4021 ggtattcata ttgatgcaat atctcaaaga aaatttggat tacgttattt tgaagctttt
     4081 cataagaaaa agcatattat ggaagcatta gctaatgaaa gtgagttgat aataccgagt
     4141 aattccaaga catatacaaa aacagaaaaa atatatataa atagtatgga cttggcacta
     4201 ggaaagatat catatgatga gtttgaatct aaacttatta aacttaatga taattagata
     4261 aaatattcat taatatacta ataaatatag attgaaagag gtgatttttt gataccatta
     4321 ttaattttag gattattaaa acaaaatcca aattcttatg gatatgagtt attagcatta
     4381 atggaaaaga agcattacaa gtacatagta aatttcacta aaggttcttt ttattataat
     4441 ttacagcaac ttgaagaaaa aaagtttatt gaaaaagttg aaaataaaca atctaataaa
     4501 aatagagaaa ctcataacta cataattact gaacttggat gccaggaatt tgaaaaatta
     4561 atgtataagt atggttctaa aacagagtat ataaatctat cattttacgc aacactatta
     4621 tttgcaaatg attataaaaa tgaaaagtta atagaattat tggaaacgca aattaagcaa
     4681 actcaagtaa gaatttctct attaaatgaa tccatagaaa atgacaaaac aaattcacat
     4741 tattttaata gaatgctaga aaattctcgt tctcaccatt tagttaatat tgaatggttt
     4801 gagaagttgc taaatgatat tagagattga ttttaataat aaaaaaacat acatatatat
     4861 tttatataaa tgtatgatat atagattaaa aataaggcag ataattttca attgaatatc
     4921 tgtctttttg ttatataaga tgttaaaaga aacttatact atgtaagtaa aatttataaa
     4981 tataaagttg ttcattgttg aattacagac acttaagatg aaaagtatga catttgatat
     5041 aataaaattg tttaagtatt aattatgagt attaattaat ggtatagata attttaatat
     5101 aactgtattt ataaataatt ataaaataat aaattaattt ttttatactg acttttttat
     5161 agtataaatc gttatatata atgacggagg tgataatagt gaatgatgct cggattatat
     5221 cacaaataaa agcaggaaaa aaagaattgt ttgatactct aataaaaaaa tactatgata
     5281 gcatctactg ttattgttat cgccatgtag acaataaaat gactgcacaa gatttaactc
     5341 aagatgtatt tgtaacagtt ataaataata tagaaaatta taaacattat ggtaagtttg
     5401 aaaactattt gtatgttatt gcaaaaaaca agtgtaagga tttttggaag aaaaaacaac
     5461 caatatattt agagtcatta gataaggaga tatcaaatca agaattatct gatatagaaa
     5521 ataaaatgtt tataaaagaa gctttgacta aattaccaga attagaattt gaagtgataa
     5581 ttttacggta ttaccatgat ttaagattta aggatattgc taaaattatg gagtcaagcg
     5641 tctctgtcac aaagtacagg atgagtaggg cattaaaaaa actagaatta tatctggaag
     5701 gaggtaaatt atgaataata aagaaataaa aagaagtcta cagaagtata aggttcccaa
     5761 atatgattct tcagctcttg aaaagactat taaaactata catgagttaa cgcttagtaa
     5821 tacttataaa agaatgaata ataaagactt ttttatatct caaattggtt ttataagaaa
     5881 aagaacttgg attatgcaat ttctaatact atgtacaata tgtatatttt tatttagtac
     5941 atcagcagat agtaattcat tttataatag tttatccttg atttctatag catcaccact
     6001 ttttgtactg acaaatatag aagaattaac aaaagtgtat aatagtagta ttttagagat
     6061 agaattttca acaaaaaata cattacataa aattattgct actagaatgc ttatatttgg
     6121 aataagtgat ttattgttta tgattattat tttggtatct gcaaaacaaa tcattgatgt
     6181 aaacattctg tatataataa tttatatact agttcctttt aacctaatgt gtataggatg
     6241 tatggaaatt ataaatagaa ataaaggtaa gcatacaaat tattattgta tgatatacgg
     6301 tatatctctt gtattattaa tagttacact gagtaacttt agagataatc tatatcaatt
     6361 tgaagcaata aatcaatggc taattgtttt tctagtcaca gcagtaacat taatatatga
     6421 aataagaaaa acgtggagac atctttgttc atttgaaaat attattatct aagtaaaaaa
     6481 taagaataca cagtatcagg aggaatagta tggagttaag tgtagaacgt ttaacaaaac
     6541 agtataacaa taaaattata gtagataaaa ttgactttaa attaaatgag ggtatatatg
     6601 gatttttagg agaaaatgga gctggaaaaa caactcttat gagaatgatt tgcggaatat
     6661 taagtcctac atctggagaa gtaaaggtgg atggtaaaaa taatttggat atgggagaag
     6721 attttagaga tatgattgga taccttcctc aaaactttgg atactaccca gattttactg
     6781 ccaaagaatt tatgctatat atagcatcat taaaaggatt aatgcctagc tatgcaaaga
     6841 gaaaaacaca agaattatta gaaatggtag gattatcaga ggtaattaat aaaaaaataa
     6901 gaacattttc tggaggtatg aagcaaaggc taggatttgc tcaaacacta cttaatgacc
     6961 ccaaaatttt aatattagat gagccaacag cagggctaga ccctaaagaa cgagttagat
     7021 ttcgcaacct aatttcagaa ttttctaagg aaaagattgt tatattgtct actcatattg
     7081 tgtctgatgt agaatatatt gcagataaaa ttttaataat gaaaaaagga agatttgttt
     7141 taactggctc aatagaagaa gtaacttcaa agataaaaga taaggtatgg aaatgtgtgg
     7201 tcaatgagaa tgaaatagat tatatgtctg atagatattg tttagcaaat atccatcatt
     7261 ctggtggagt tgcaaatctt agaattgtaa gtgatataaa gccacatgac aatgcatgca
     7321 atttaaaacc aacattagaa gatttatatt tatattgttt caacgaagta ctataaatat
     7381 acataaggag ataggtatgt ctacattaat taaatttgag ttaaaaaaaa taataaaaag
     7441 aaagtctaca atagcagtta ttttaggaag tttattctta agcatcattc tttttgcatt
     7501 aatgccaatt caggaaaaag ctaatgatga aagtggaaaa gagtataggg gtttacaggc
     7561 tattgagcta aaaaagcaac aatctaaatt aaatgaggga tacataacag ataaaaaagt
     7621 aaaaaactca atcagagatt accaaaaaat gtttaataat ccaaataata ttacaacaga
     7681 tgaaggtggg ttttcaaatt ttaaaccaga tatttattgg aaattcttct atccaaataa
     7741 agctttttat aattttataa tagagtcata tagtaatcca aatgattata atccagaaat
     7801 aattaaatca atgcctttag aagatggagt taatttttat aaatttagaa atgaaaaagt
     7861 aaatacaata ttaaatatgg aatatcctga taaaaattac acacagaaag agaaagattt
     7921 ttggttgtct aaaaataagg atatagaaga gccttattta aatggatatt atcaaggatg
     7981 gaattatttt ctattggcta gagatttact aatttttact atagtagctg tctgcataag
     8041 tacagcttct gtattttcag gagaatacca atccggtgct gattcaataa tattatcttc
     8101 taaatatgga aaaacaaaag ttataacagc taaaatagta agttcctttt tatttggtac
     8161 gattattttt acaataaata caattgtgtc tattggtata cttcttttat cttttggaat
     8221 tgatggatgg aatttaccat tacaacttac aaccagttca attccatacc catatacatt
     8281 tttacaggca atattactaa gtgtgttaat tatttatatg atattaatac tattaattag
     8341 ttttacactt ttattatcat cttatatgaa atcacctttt ccagttttaa ttacaaatat
     8401 aatattacta tttgtaccaa tatttttaaa atatagtgaa acaaataagt tgtataatca
     8461 aattctcaat gttttaccat caaatgctat ttcattttca tttactaatt atgtagatta
     8521 taaatttggt aatttaataa tagctttacc tacaatgatt atttttacat gcttagttct
     8581 tacagttgtt tttttacctt ttattaaaaa taattttaaa aaacatcaag ttaactaaaa
     8641 tatatattat aaaaagaagg tgtcttaaaa tgaacttttt agcttattag gcactcttct
     8701 ttgtattaat ttaaatatat tttagtatta gattaaatat gttttcatca ttttaacaat
     8761 gaaaaagagc tttatctcta ttttgagata gctcttataa agttaaatat taaattgata
     8821 actataaaat atgtagtttg aatttataat ttgtgttata atcatactga tt
//
LOCUS       DAYATN010000145         8778 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.146, whole genome shotgun sequence.
ACCESSION   DAYATN010000145 DAYATN010000000
VERSION     DAYATN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.146"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            73..414
                     /locus_tag="ACGI8A_003169"
     CDS             73..414
                     /locus_tag="ACGI8A_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFH0181148.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            565..978
                     /locus_tag="ACGI8A_003170"
     CDS             565..978
                     /locus_tag="ACGI8A_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HFH0181149.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1180..1821
                     /locus_tag="ACGI8A_003171"
     CDS             1180..1821
                     /locus_tag="ACGI8A_003171"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFH0181150.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1845..3098
                     /locus_tag="ACGI8A_003172"
     CDS             1845..3098
                     /locus_tag="ACGI8A_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFH0181151.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3144..3794
                     /gene="cmk"
                     /locus_tag="ACGI8A_003173"
     CDS             3144..3794
                     /gene="cmk"
                     /locus_tag="ACGI8A_003173"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFH0181152.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3845..4441
                     /locus_tag="ACGI8A_003174"
     CDS             3845..4441
                     /locus_tag="ACGI8A_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFH0181153.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4454..5293
                     /locus_tag="ACGI8A_003175"
     CDS             4454..5293
                     /locus_tag="ACGI8A_003175"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFH0181154.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5538..6692
                     /locus_tag="ACGI8A_003176"
     CDS             5538..6692
                     /locus_tag="ACGI8A_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HFH0181155.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            6998..8695
                     /gene="ade"
                     /locus_tag="ACGI8A_003177"
     CDS             6998..8695
                     /gene="ade"
                     /locus_tag="ACGI8A_003177"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFH0181156.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3596 a          838 c         1568 g         2776 t
ORIGIN      
        1 atatatttcc gatagattat attgataaaa aacactaaaa ttaaattaat aaaagtactt
       61 ggagggatgg aaatgaaaga ggaaaatttt attaatgttg aatacgagga taaacgtttt
      121 tcaattaata gtaaagaagt tttgtacata gaaaagcaag agtatgaagt aaagatttat
      181 actgaaagtg gtaattatga agttggagaa aatgctcctt gtataagaaa actattgaaa
      241 gaagaaaatt ttttcaaatg cagtaatagt tatattgtaa atctagacaa gtgtgaattt
      301 tatctcagca ataattgtat ttttgtgaat aataatttta taagaatttt aggttcagaa
      361 agaaaaaaga aattaaaaaa gaaattagaa aaattatatg attatgaaga ttaatattgg
      421 gaggctgtat tttatacagc ctctttattt attgattgta ttattaataa attttaaatt
      481 atgataaaat ataaaacaag taacttattt cagaataaaa tactttttaa aagaaaggga
      541 cgagatatgg gagattataa taaaatgctt gaattaaaga ataacttagg tatcagtgaa
      601 agaaagttaa aatatccttg tatctataaa catttcaaag ggaaatatta tgctacaatg
      661 ggtcttagca aggctattga tgatatagaa aacatatgtg aaatatatgg taaagaaaat
      721 ttaatccaaa acagaaataa atataaatta gttattagac atactgaaag agaagaagat
      781 atctatgttt atagggattt agatggaaac ttttatcata aaaaagaaga agatactaat
      841 gatttagttt tatacaagac cttatatgat gatacaggaa tatttgcaag acctttggat
      901 atgtttttag aaaaagtaga tacagataag tatgtcaatt caattcaaga atacagattt
      961 gaagaagtat ataaataata taagagaaat atgtttataa atttagaaaa attctttaac
     1021 agtacaataa tttgcagaaa tacaaatgag atttattgta aataatatgt tagattagat
     1081 tataaaatag aaacaaagta aagtattata tgatataatt aagttttgag aagtgataat
     1141 ttatatttaa taaaaaatag gcatatatta ggaggacata tgggaaatac tttttatata
     1201 gtaagacatg gacaaacgga ttggaatata ttgggaaaaa cacaaggtca tggtaattct
     1261 gaccttacgc ctcaaggtat agaacaggca aaagaacttt ctgaagatat tggcaagtat
     1321 tcaattgatt atatttttag tagcgacctt gggagagcaa tgcaaacagc tcaaatttta
     1381 ggagataagc tcaatattga agttcaaaaa actgaggcac ttagagaaat gggattcgga
     1441 gtttgggaag gccttttaat aaaagaaatt caaaaagatt attcagatat atatgcaaca
     1501 tggagaaatg aaccacactt agtaaatata ccagaaggcg aaactcttaa gattataaag
     1561 gaaagagtag atgcttttat aaaggaacta aatgaaaaat atgataataa aaatataatt
     1621 cttgttacac attcaattac attgagggtt atgttgttgt catttttaga atcaggtatg
     1681 gaaaatatat atagaattaa gcaagataat actgctttaa atatagtaga atttaaagat
     1741 tatgggcctg ttatagtaaa aatgaatgat acaagtcata taaaaaatca tgtaaagata
     1801 aataattctg cactggagta ggaatcaatt ataaggagaa atttatgagt aaagttatag
     1861 tagttggtgg aggagcttct ggaatgatgg cagctttgtc agcttcaaaa aacaataatg
     1921 aagtaatatt agttgagaga aatggagaat taggaagaaa acttagggca actggtggcg
     1981 gtagatgcaa ttttaccaat aatagagaga tagaagattt ttttgataaa gttgtcagta
     2041 ataaaaaatt tctctatagt agtttttata catttacaaa taaagacctt atttcgtatt
     2101 ttgaaagtag aaatttggaa tataagattg aagaagaaaa tgaccacaaa gtatacacta
     2161 aaaatgataa atctatagaa gtaatagaag tattaaataa agatttactc aatcacaatg
     2221 taaagataat gtataataag aaagttatag atattataac tgaagaaatt gctctaaaag
     2281 atgattctaa taaggacaag tcaaagtatc taataaaagg gattatttta gataatggag
     2341 ataaaatact aggagacaag gttattatat caactggagg agtgagttat tcaaagacgg
     2401 gttcagatgg ttcaatgtat aagattctta aaaagcatgg acatacatta aacaaactat
     2461 atcctgcttt agttccactt acaatagagg aaaaatggat taaagattta caaggaatat
     2521 ccatgaagaa tgtggaaata tcttgtaaga ttaagaaaag gaaaatatca aaatctggag
     2581 acatgctatt tgcccatttt gggataacag gaccttgtgt tcttataatg tcttcttata
     2641 ttaacaagat tattgaaaag gaaaaggtag aattaaatat agatttctta ccaaatttaa
     2701 gtactgatga aatctctagt ataatcagag catttccaaa taaaaatgtt ctaaataatc
     2761 ttaagcaaat actacctcaa aatttcttaa aagagatttt ttctctatta tctttagttg
     2821 ataaaaaggc aagtgatttg tctaaggcag atgaaattag aattattgag tatataaaaa
     2881 atatgaagct aacttgtaat gggactacag gtataaacac tggtatggtt acttctggtg
     2941 gtatttcagt taaagaaatt aattcttcta ctatggaatc taaattagtt aagaacttat
     3001 tttttacagg tgaagtaatt gatatagatg cagaaactgg tggatataat ttacaaatag
     3061 cattttcaac tggatatctt gcgggtataa gtgtataaat ataatttttt aataattaaa
     3121 tatgaataaa tagaggtgta tatatgggta atttagttat agctgtagat ggtccagcag
     3181 gagctggaaa aagtacaata gccaagatag tagcaaaaaa gctaaatata aattatatag
     3241 atacaggtgc tatgtacaga gcagttactt ataagtgttt aaaaagtggt attgatgtca
     3301 ataatgaaaa agaagttatt caaatagctg aaaattcgga tatagatttt aaagataaca
     3361 atatatattt ggacaaagaa gttataaatg aagaaataag aacaatagaa gtaagcaata
     3421 atgtttctaa tgtagcaaaa ataaaagaag taagacagtt gatggttgaa gttcaacgaa
     3481 aaataggaat gaaaaattca gtaatactag atggtagaga tataggttca tatgttttcc
     3541 cagatgcaga ttacaaattc tttttagtag ctactccaga agaaagagga aatagacgct
     3601 ataaagaact ttgtaataaa ggatataata ctactttaga agaagtaatt gaggatataa
     3661 taagacgaga tgaaattgat tcaaatagag aatttgcacc attggtaaaa gctaatgatg
     3721 cactagaaat agatactaca ggaaaaacta tagaagaagt tgtagaagaa gttgtatcta
     3781 aaataaatct ttagtttttt atgcatcata atgaaaataa atgtagtgtt gaagggagat
     3841 aaacgtgaat ttttatagat ttgtaataaa tatttttaag ggtttttcta aaatattttt
     3901 taaatatgaa gttataggtg cagagaatat tccagataga ggaaatatag taatagcatc
     3961 aaatcataaa tcgaatcttg accctatatt cttggctgct gccatagaaa atagagaaat
     4021 agctgcaatt gcaaagaaag aattgtttaa agtaaaacca ttgggattta tattaaaaaa
     4081 attgcatgtt atgcctataa atagagaaaa accagatgta tctacaataa aaactatttt
     4141 aaggtcagtt agagacggtt atgtattagg aatatttcca gagggtacta gaataaaagg
     4201 agattctttt ggtaaggcta aggcaggtct ttctgtattt acaataaaaa gtaaatctaa
     4261 agttgttcca gtatcaataa tatctaaata taaacttttt agtaaagtaa ttgtatatat
     4321 tggagaacca atatcatttg aagaacattt taaggaaaaa ttgagtaatg atgaccatga
     4381 aaggatatca caagagattt tagaagtgat aaaacaaaat tactttaaat attcaaaata
     4441 agggggtaaa gttatgaaca taaaaatagc aaaaaatgct ggcttttgtt ttggagtaaa
     4501 aagagcaatg aaaatggcat gggatgaagt tgaaaaaaat gattctggca tatatgcttt
     4561 aggtcctctt attcataaca agcaagcagt agcaaaatat gaagaaaaag gtcttaagac
     4621 tgtgaatgaa attgatacaa taccaaatca tgaaaatatg ataataaggt ctcatggagt
     4681 tcctgaaaat atatataagg aagccaaaga taaaaaatta aaaattgtgg atactacatg
     4741 tccatttgta aagaaaatac atacagtagt aagtgaatat cataataaag gctatgaaat
     4801 aatagttatt ggagatatga agcatccaga agttataggt ataaatggat ggtgtgaaaa
     4861 ttcagctata ataataaaga ccttagaaca aatggaaaat atggaatttg ataactccaa
     4921 aagatattgt cttgttgcac agactacaat aaatccagag ttgtatataa gtatagttaa
     4981 taaactatca gataaattag aagaaatagt atttaatgat acaatttgtt cagctacaaa
     5041 aacaagacaa gaatcagcta aagagttagc taaagaagtt gattgtatga tagttatagg
     5101 aggtaaacac agttcaaata ctcaaaagtt agtaaaggtt tgtgaagact tagtaccaac
     5161 ttttgcaata gagactaaag atgaattgga tgtaaatacg cttaaaaaat ataaaaattt
     5221 gggtattaca gcaggggcat ctacacctaa ttggataata gaagaagttg taacattcct
     5281 tgaaaattta taatttgata ggtaataaaa ataggtggac attgttataa tttatcataa
     5341 tatccacctt tgatttcata ttacatgtgg taaatagata acctaatatc aaaaagtgtg
     5401 tatcttaaga tgaaaaacta agagtataag atgcgcactt tttaatatta gaaattcaaa
     5461 agaaatattg atattgatta tcaatagaaa atagaatata atataattgt tgcaaaatta
     5521 gtctaggagg taaattaatg gaaatgttga tatccgcttc tgaagaggcg tttttacatg
     5581 taggttctat gattggattt tttattttgt tatttggata tattaattat aaaactagtg
     5641 gaaattttac taatatcata tcaaaaaaca gaaaatttca acccttaata ggtgctttaa
     5701 ttggagctat accaggttgt ggcggaagtt tggcaataat gccattgtat ataaatggaa
     5761 aattaagctt tggagcaata attgcaagtt taatagctag tatgggagat gcagcatttg
     5821 tacttatttc atcaaatatt aaaatgtatt tttttgtcac tatagttagt acaattacag
     5881 gaataataac tggacaatta gtggattact ttaaactaga agaaaaatta gggttaaaaa
     5941 accgtagaaa atcagataaa tatgatataa gtaaaaataa ccctaaagaa aataaagaac
     6001 aacataatca tgatgaaata attttagaca ctctagctaa atctcatgga aatacaaata
     6061 gacttgcatt tataataaca catggaaagg gatataaaat ttatatagga attatagtaa
     6121 tcggttttat atttatgtca cttgctcact ctggacttaa tcttcctata attgagaagt
     6181 tacattcatt ggaagaagta atagcagtga ttggaatatt attttcaata atttatatgt
     6241 ggtgttttaa aaaggtattt aaaaattcta atcaaaaaga ggaagaaaat aaaaagattt
     6301 ctttgcgaga aatgcttatc cattctgttg gagaaatatc atttgtaatt acatggattt
     6361 ttattgcata tcttatttac gatttgataa tattaatttt aggtggagat gaatacttag
     6421 ttaaattagt attatcaaca ggtgtaataa gtgtatttat aggtgcaggt cttggactta
     6481 ttccaggttg tgggatacaa attgtgctta tgtcatttta tttgaaggga actataccac
     6541 ttggtgcagt gatagccaac tctatttctc aagatggaga tgcacttttc ccacttttag
     6601 caatggataa aaaatcttct ttatgggcta tgattattac tactatacca gcaatacttg
     6661 taggtatgat agtttatgca tttttaggtt aaatataatt atgaataaag aagtattaaa
     6721 aaaataataa agcacataaa tttatttata agaatagtga agattaaata taaaaataaa
     6781 aaattcaata tgaattaaat tattgcttaa atattaatga gaacatggga gataatatta
     6841 ttcctcatgt tctctctatt taattaaagg aattatggaa caaatttcaa gtgatacttt
     6901 ctatcaaaac tcatatataa tatatattaa taaattaaac tataatacgt aatacagtca
     6961 gtatattgat ataaaaaact taggaggagt tgtaaaaatg ggtaatttaa aaagacagtg
     7021 tgatgtttca agtggaagag aaaaagctga cattgtacta aaaaatggga caatcataaa
     7081 tgtatttaca gaagagttaa taacaggaga tgtagcaata gtaggagaca ctattgttgg
     7141 aattggcgat tacaagggca atgtagaaat tgattgtagt aacaaatata tatcacctgg
     7201 ctttattgat gcacatatgc atattgaatc tacaatggta atgccaattg aattatcgaa
     7261 aaaattatta aaatcaggta cgacaactat catagccgac ccacatgagt tggtcaatgt
     7321 aaaaggagtt agtgcaatag attttttatt agaatcgaca aaagatatac cacttaatgt
     7381 atatattatg gtgccttcat ccgtacctgc aacaagtttt gaaactaatg gagttggtaa
     7441 atttagtgca aaagacatgg agagttatgt aaataatcct agaattttag gtttaggaga
     7501 ggtaatgtgt tttaatgatg ttataaattc tgaaaatgaa atcttggata aactagagct
     7561 ttttaaaaat aaagttgtag atggacatgc acctaatata aatggtaaat cacttcagac
     7621 atatgtatgt gcaggaatag aaaatgacca tgaatgtata acttttgatg aagtatatga
     7681 aaaattgaga gcaggattaa aaattttaat aagagaagga agcgcagcaa aaaatttaaa
     7741 gtctatagta agtggtatgc taaagcataa cttgccaatt gaagaattta tgttttgcac
     7801 agatgataag cacttagatg atatagaaaa acaaggtcac ataagatgga atataaaatg
     7861 tgcgattgat ttaggtatgg aacctgttag ggctataaaa gttgcaactt ataattctgc
     7921 aaaagcatat gggttaagga aaattggagc aataggtgcg ggttataagg cagatattgt
     7981 tgtattgaat gatttagaca aaatggaagt ggatagtgta tataaagatg gtaatttggt
     8041 aaacgaagag atgttttcta attacaatta tgagattaaa gataaagaat tgcttaatac
     8101 agtcaagttt aaatatatta ataaagaaaa aatacagtta aaagtaagtg aaaaaaatta
     8161 tgtaatggag attgtacctt atcaaatttt gacaaataag gtatatgaat ctttaccatg
     8221 tgcagatgga tattttgttc caaataaaga gtattcaaaa ctttgtgttg ttgagagaca
     8281 tagaatgact ggaaatgtag caattgctcc actaaaagga tttggcataa aaaatggagc
     8341 aattgctact actgtggccc atgattctca taatattata gttgcagggg ataatgatga
     8401 tgatattcta gtagctataa attacttaaa ggaaatacaa ggtggttatg taattgtatc
     8461 aaatggaaaa gttcttgcac acctaagtct tcaagtagct ggattaatta gtacttttac
     8521 agctgaagaa gtacaagaaa ttactgataa tatgttggag atagcaagaa aaatggaagt
     8581 tccagagtat gtagaccctt ttattacact ttcttttatg gcacttccag ttgttccaca
     8641 aataagactt acagatttag gtctatttga tgttgaggaa tttaaattta tatagatagt
     8701 ataaaagtaa taataaatga ggctttctca aaatagagat aagtctcttt ttattgttga
     8761 aatgatgaaa atatattt
//
LOCUS       DAYATN010000146         8513 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.147, whole genome shotgun sequence.
ACCESSION   DAYATN010000146 DAYATN010000000
VERSION     DAYATN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.147"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            106..2007
                     /locus_tag="ACGI8A_003178"
     CDS             106..2007
                     /locus_tag="ACGI8A_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0181157.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2010..3053
                     /gene="ypdE"
                     /locus_tag="ACGI8A_003179"
     CDS             2010..3053
                     /gene="ypdE"
                     /locus_tag="ACGI8A_003179"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFH0181158.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3068..3373
                     /locus_tag="ACGI8A_003180"
     CDS             3068..3373
                     /locus_tag="ACGI8A_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFH0181159.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3470..3805
                     /locus_tag="ACGI8A_003181"
     CDS             3470..3805
                     /locus_tag="ACGI8A_003181"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0181160.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3837..5159
                     /locus_tag="ACGI8A_003182"
     CDS             3837..5159
                     /locus_tag="ACGI8A_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0181161.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEGLLDDLGLDF"
     gene            5169..6239
                     /locus_tag="ACGI8A_003183"
     CDS             5169..6239
                     /locus_tag="ACGI8A_003183"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFH0181162.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6244..7431
                     /locus_tag="ACGI8A_003184"
     CDS             6244..7431
                     /locus_tag="ACGI8A_003184"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFH0181163.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7628..8500)
                     /locus_tag="ACGI8A_003185"
     CDS             complement(7628..8500)
                     /locus_tag="ACGI8A_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFH0181164.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
BASE COUNT         3305 a          941 c         1503 g         2764 t
ORIGIN      
        1 actaggatat aaaattctta aataaaaaga aaaggttgtt tttataaaaa atatttttta
       61 taccacttta aaaaacacaa taataacact gagggaggtg gggatatgag agaacgcatt
      121 attcttgaaa cattaaaaaa tagtagtggt tatctaaaca tagattattt tgcagataag
      181 ttgggggtat ctactcgaac tatcagaaat gaaataaaaa agatagaaac tataaaagaa
      241 tgtaatggtt ttaaattaga atataagtct aaattaggat atattctaaa tattaaagac
      301 caagataaat tcgagcatta tctaagaaac ttaccctcag atttagttga aaatccagaa
      361 caacggttgg agtctataat agtagagctt ttagtaaatg aaggttataa aactatagaa
      421 cagttaagta aaaaatttct agtaagttca tcccaaataa aaaatgattt aaaaaagata
      481 gatgaaaaga taaaggatac agaattaaaa ctagagcgaa aggctcatta tggaattaaa
      541 atagaaggtt ctataaaaga aattcaaagt atattggtgg atagttattt tagagggaat
      601 agaaatataa cagagtacag gaataagttt atagataatt taaaattagc taatattaga
      661 agtacaataa aaaatgtact taatgaacat gatttagaag caaatttaac agaacttgag
      721 gaaatattag cacaaattat tatattgtat ataagagtta gtatgagagt agtgggaaat
      781 gctagcgatt taaaattata tacagaagat ttaattatag ataaattatt agataaaatt
      841 tttagagata aaaaatataa ccttaattat gatgaaaagt attatttaaa ggagcttata
      901 aaactaaaga ctaaagataa gaaagccact ataaagaata ttgataagaa taaacttcaa
      961 gatattatgt tcgaattttt tagagagata gataaaaaat ataactctaa ttttttagaa
     1021 gataaagagt tttttaactt attttactta catattgcat gtttaataga acgtataaag
     1081 aagaaccaca agataataaa tcctttttca gttaaaataa gtcaacagta tccaactgtt
     1141 tttaatttat ctattcaatt ttcaaaaatc atagaaaatg agtatcatat aaaaataagt
     1201 caagatgaaa tagggtttat agctactcac attgctgttc cttttgaaaa gagagaagaa
     1261 gctaatttta ataaaaaata taaaatagca ataatatgct cttctggtgg aggtagtgca
     1321 tttttgataa atttaagact taaagaaatt tttccaaatg ctgagataag aaatttttca
     1381 ttactagaag aaaaagctgt aatagaattt gcaccagatt tgattttttc aataacaaat
     1441 cttttatttg aaataaatgc tccagttata cttataaatg aaattttaga cgaattggat
     1501 tatttagata ttaaagaaag tgttagattt gcagaccata taggaaatat atcaagccca
     1561 aaacaatata tattaggtct atttgataag aatcacttta ggtgtataaa agataaagct
     1621 aagtataagg atatccttga tagtatgtca aaatgtatag ttgacgaggg agcttgttct
     1681 ccaacctatc ccaaagatgt atgggaaaga gaaagttatt tgagtactat ttatacaaat
     1741 ggggtggcta tacctcatcc aatcgagatg acaggaaaca aaaatataat atcagtagct
     1801 ttgatacaaa ctgatattat ttatgaaaat agggtgccta aaattatttt catgatttca
     1861 ttaataaagg gaaatttaga acttcataag caaatatcta aatatttaag taagattatg
     1921 gcaaataagg atatggtaga catgctaaat aagtcacaat cttatgaaga gtttatgtat
     1981 aagttaaaaa tctatctggg aggttagaca tgaatataga gttttttaag aggttgtcag
     2041 aagcagatgg tatagcatct aatgaagaag aggttagaca tgttttattg gaagaactaa
     2101 aagcttatag tgataaaatt atctgtgatg ggctagggag tatcatcttt tctaaaataa
     2161 aagatgagag tgcaccaaac gtaatgattt gtgctcatat ggatgaagtt ggatttatgg
     2221 tgagaagtat agacaaactt ggtatgattc acttaataac aataggagga gtaaaacctc
     2281 ttgcacagtt tgtacaaaaa gttagaatta caacaaaaga agggaagaaa atccccgctg
     2341 tcataaatgc tacatataat aatggtaagg ctgaaaatat atatgcagat attggagcat
     2401 atacagaaga agatgtatat aacctaggga ttaatgttgg ggatatggta acatatacaa
     2461 caagctttga ggaatttact ttaccagacc gattagtggg aaaggctttt gatgatagga
     2521 taggatgttt tgtgatggga gaagttttaa aagaacttag gaaagaaaat ctaaattgta
     2581 atattcactt tgcggctaca agtagtgaag aggttggaat aaggggagca aagacatcaa
     2641 cacagttaat aaatccagat atagtatttg taatagatgt agcatgtgct aaaaatgagt
     2701 ttgtgagaga ctatacaaat aagaaacaaa ttgataaagg tgtaatgcta atgcatagag
     2761 ataggactct agtccctagt agaaaaatga tagattatgt aatggaaatt gctgataaaa
     2821 ataatatacc acttcaacat gatatgtttg aatcaggtgg gactgatgga ggagaagcac
     2881 atctcgtaaa tgaagggaaa ccttgtgtag taacttgtgt accagttaga tatggtcatt
     2941 gtgcattttc aatagtaagt aataaagatt tggagaacat tataagattg tatacacagt
     3001 taattttaaa ttttgatgaa gaacaatata aacatataag aaattttatc taaaggagaa
     3061 taagaaaatg tcaacagaag tacaaataat ggaacttata tcaactgcag gagagagtaa
     3121 atcgaaagct tttgaggctt taaaaaaagt gaagtcaaaa gattttaaag gagcaaaatt
     3181 gttaatagag gaggctcgta aaatagatat tgaagctcac aggatacaaa cagaattaat
     3241 aactcaagat ttaaatgaag atgttgaaag tccagcaatg agtcttttaa tggtacatgc
     3301 tcaagaccac tatatgactt ctcagctagc aaaagacttg atagaggctt tgatatattg
     3361 tcttgataag taattgtaat atagaaatag attatttatg taaaaattct tttaaaagtt
     3421 tagatgcttt taagagataa taaaaataca aactttagga ggaattatta tgaaaatatt
     3481 attatgttgt gcaggtggat tttctactaa tatgttaatg caaaatatga aaaaggtggt
     3541 aaaagagagt gctaaactca atgaaaaaga ttttgagttt acagctatac cagcagattc
     3601 aatagaaagt gaagttgatg attgggatat agtacttata ggtcctcaaa tagctcacaa
     3661 aatagacttc ttaaaaggaa tattagagcc aaaaaataag ccttttgcag taatagataa
     3721 agatgtatat ggaaaaatgg atggagctac agttatgaag ttggcattaa taacttataa
     3781 gaaacatcaa ttagctcaag gataataaaa ataatgaggg aaaagggaga ttagatatgt
     3841 tttcaaaatt tgaagcattt atgaatagag tatttatgcc tttagctcat aaggttgaca
     3901 atcaacgtca ccttggttct ataaaggctg gtatggttgc tatgacacca ttcacaatat
     3961 taggaagtat atttgcaata cttccagcac ttccaaatat gcttggagaa aataatccaa
     4021 taagcaaatt catacttagc aattcagcaa tattggattt accagtaagt ctttccatag
     4081 gtttaatagg attatatgct tgtatgtgta ttgcttttaa tctggggaac tactataaat
     4141 tatatatacc aggttgtgtt gcactatcaa ctttttcttt tttattccta gtagcctcat
     4201 ttacaccaga tggagcttta gaaataggaa atttaggagc taaagggcta tttacaggta
     4261 tgatagcagg gcttggttct gtggagttat ataatttttg taataagaag aatatcacaa
     4321 taaaaatgcc agaaaatgta cctgattttg tatctaggtc ttttgaatta atacctacta
     4381 ctataatagt ttgtggaact tttatagttg ctcgttttat ttcattaaaa gtatttggag
     4441 aattacctcc acagatactt actcaatttt tatctccatt agtaggaagt atggataacc
     4501 catggtcagt tcttgcactt aactttgcta tatgtactat attctttttt ggaatacatt
     4561 catcagtatt tagtcctata actagaccta taatgattac atttatagct gaaaacatag
     4621 cagctatgca ggctggagaa acaatacctc atttctttac agcaggagct tcaagtgcat
     4681 tctttggatt tacaggatgt ggaattagta taggttgtgt aattgcatgt atgttatcaa
     4741 aaaatgcttc ctataagaag attggacgtg tatcattatt ccctgcatta tttgggataa
     4801 atgaaccaat attatttgga gcaccgataa tattaaatcc aataatgttt ataccacatg
     4861 tatttggtgg agctataata ggtacactac caatgttttt tatgcactgg ggatggttag
     4921 caaagccagt atttgaccct ccatatgtag gggtattctt agaaggattt ttaactaatg
     4981 gagattggca ttctatatta gcaaatgcat tgcaattagt attagcagtt ttattatatt
     5041 ggccattctt taaaataatg gaacgtcaac aaaataataa aaaacaaaaa gaagaaacta
     5101 agaatatatt ctctaaagaa gaagaaggct tattggatga tttaggattg gatttctaat
     5161 attttgttat gaataataca atggagagat tagtatcctt aatctataaa agaggtgttg
     5221 acgctctttt gattaaagca aaaaatacta aaagatatat aggagcttta tcaggtagtg
     5281 gtgtatatgt gcttgttact aaggataaaa attatcaaat tctagatggc agatatattg
     5341 atgaagcaga taaaaaaacg actggtttca taaaaaggat tgtatctcaa ggttcttata
     5401 taccaactat aattgagtta ttaaaagaat taaatataaa aaagttagca atagaaaacc
     5461 aagctatgag tatacaagag tatctttcac taaaaaatga aggttttgaa atcaatttaa
     5521 taacaaatga attatggaag ctaagggcta taaaatctaa agaagaaatt aaacttatta
     5581 aaaaggcttg tgagattaca gatgaagtat ttgatgaggt tatttctgaa attaaaaaag
     5641 atatgactga gctagaagta tcagctctaa ttcagtatca tgcattaaaa aaaggtgcaa
     5701 gtggaatgtc ttttgaaaca atagtggtat caggagaaag aggtgcaatg ccacatggaa
     5761 gacctacaaa caaaaaattg aaagtgaatg aagctataac aatagatttt ggtgttgttt
     5821 atcaagggta tcaatcagac atgactcgta caatatctat aggcaaacct cctaaaatta
     5881 ttaaagagat atatgatgta gtattggaag cacaattatc tgcaatagag tcaataaagg
     5941 aaggaactag ggcaagtgat gttgacaaag tggctagaaa aattatagat aaacatggat
     6001 ttggcgaata ttttaatcat ggactgggtc atggtatagg tcttggagat ggtgaagttc
     6061 ccactttaaa tccaaatagt gaagatatat tagtggaagg aatggtaatg tcttgtgagc
     6121 caggtattta tattccaaat gtagggggag ttagaataga agatgatatt gttataatag
     6181 atggaaaagg tgttccactc aataagacat ccaaagagtt tataatatta ggagagtaaa
     6241 attatgaaat ataattttaa taaagtatta aatcgtaaag aaggaaattg tagaaaatgg
     6301 agcaaccatg tgattaaaga aaaatttggc ttaaatgaag atgcaattcc tatggattta
     6361 gctgatatcg attttgagtg tgctcctgct ataaaagaat ctatcttaaa aagagcatca
     6421 gtaggagatt atagttatac ttttatagga gatgacttct atgatgctat tataaattgg
     6481 aataaaagaa gatttgatgt atatatagaa aaggattgga taaaattaac ttttggtact
     6541 gtttcaaccc ttcattatat agtccaagca tatactaaag aaggagaggg tgtattgata
     6601 aatacacctg cttatgaccc ttttgcagag gctgtagtaa ataataatag aaagttgtgt
     6661 tgtagctcat taaaattaga taacaataga tattatttag atttagagga tatggaaaat
     6721 caaatgaaat acgagaatat aagattgctt atattctgta gtccacaaaa tccatcaggt
     6781 cgtatatgga caaaagaaga actatatcaa gtgtcagagt tatgtttaaa gtataatgta
     6841 attttggtaa gtgatgaaat tcatagagat ataatattta aagattatag atttgtatct
     6901 ttgtggaatg ctcatccaga aatttataaa aatagtataa tctgtgtatc tcctaataaa
     6961 ggcttcaact taggtggatt aaaaacatca tatgtattaa taagagatgt aaaaattagg
     7021 gagactttat tagaaaggct aaaaagtaat tcaataactt ctccaaatgt atttgcaata
     7081 ccagcaatag ttgcagcata taatgaaagc gaagaatggc tagatgctat gactagttat
     7141 gtagaggaga attttgaaat agtttatgat ttctttgaaa ctaatatacc aaaagctaaa
     7201 gtgatgaagt cagactcttc ttttttagcc tggatagatg taagagaagt atttagaaat
     7261 gaagaagaat ctaaagaatt ttttagacat gcaaacttaa ctatggtagt tggaagttat
     7321 tttgtgaaag atggagatgg atttattcgt attaatgtgg ggtgtcctag agaaacatta
     7381 aatgaggctc taaatagaat caaaaaaacg tatataagta tgtattgtta aatcataaaa
     7441 aaagtatcta tggtttatat aaaaacacat tatttagtta tcaaatatta ctggaatttt
     7501 gtattccagt aatatttttt gattaaggaa tattattgag gaatattaaa taatattatt
     7561 tcataccctt atattaactt atataaaaat attttattat tttgggaacg ggttagctaa
     7621 cctttatcta gtgtatattt atattgtaag aagcagtaaa aacttttcct ttactaagtt
     7681 tattaataaa cttcttatct ttataaattt tgttagaatt tatactatca gcaagaccat
     7741 accaaggttc tatacataaa aaaggagcag tagaattagt ttcactataa taaggagtcc
     7801 aaataccaac tagagggaaa tcttccatag atatagttac atatttacta ccatcattat
     7861 tacatataga cacttcatct atgttggtgt atatcaaagc atcattcttg aatacttctg
     7921 gatttaactg taagtttttt aattttccaa taggtattat atcatctgta aaagaaccat
     7981 ttaaattgat tttttctaca tcatttttac ttttaaactc aaggtagtgt ttagaaaaat
     8041 cattttgttc gtaaaaagga aggttgaatg caggatgtcc acctatagaa aagaatatat
     8101 ctttagaatc agtatttttt acaatccatt ctacctcaat actattatca tttaatgtat
     8161 agtttatgat tagctcaaaa gaatatggat acttctttaa agtagattca ttatctgtta
     8221 atttataagt tacaaatgta tctcctttat ctacaatctc aaagtccata tctctagcaa
     8281 acccatgttg agtcatgcta tataaatttt cttctattat tgtttcatta tcaaaaagtt
     8341 tacctactat agggaacaat ataggtgatt gtctacccca atatttttta ttaccatccc
     8401 aaagaatgtt tttgtcgtat tttttagaaa atattttagt tagttcagca ccaaaactgc
     8461 ttgattcgat aattaaattt tcattttgta atatattcat ttaaaatctc ctt
//
LOCUS       DAYATN010000147         8504 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.148, whole genome shotgun sequence.
ACCESSION   DAYATN010000147 DAYATN010000000
VERSION     DAYATN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.148"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            231..1310
                     /locus_tag="ACGI8A_003186"
     CDS             231..1310
                     /locus_tag="ACGI8A_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFH0181165.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIVKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="ACGI8A_003187"
     CDS             1634..2002
                     /locus_tag="ACGI8A_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFH0181166.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKSLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="ACGI8A_003188"
     CDS             2119..2922
                     /locus_tag="ACGI8A_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0181167.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYMGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3314..4204
                     /locus_tag="ACGI8A_003189"
     CDS             3314..4204
                     /locus_tag="ACGI8A_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0181168.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
     gene            4617..5060
                     /locus_tag="ACGI8A_003190"
     CDS             4617..5060
                     /locus_tag="ACGI8A_003190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0181169.1"
                     /translation="MIEIKTIEINKKSRIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5232..5402
                     /locus_tag="ACGI8A_003191"
     CDS             5232..5402
                     /locus_tag="ACGI8A_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181170.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5775..6872
                     /locus_tag="ACGI8A_003192"
     CDS             5775..6872
                     /locus_tag="ACGI8A_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFH0181171.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            7077..7565
                     /locus_tag="ACGI8A_003193"
     CDS             7077..7565
                     /locus_tag="ACGI8A_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HFH0181172.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7651..8058
                     /locus_tag="ACGI8A_003194"
     CDS             7651..8058
                     /locus_tag="ACGI8A_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181173.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            8059..8277
                     /locus_tag="ACGI8A_003195"
     CDS             8059..8277
                     /locus_tag="ACGI8A_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0181174.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
BASE COUNT         3340 a          844 c         1504 g         2816 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagccttta
      601 tcatcaagga cgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggct gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacccg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgtaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctac taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagcc atataaatta gaatttgtag
     1621 aggagaaata taaatgaatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaatca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gatagacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gttacgaaaa aaaagaactg tgtcatctca tgtatatggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat atataaattt
     3061 ttaagcgaaa tacatcaaaa gaaggattag aattagtgga gacagaacag ctattataga
     3121 ttataaataa atagttttaa aggagaggta ctacgaatat agaggaaggt aaatcatacg
     3181 ttattcaagt attataaatg attagtttaa aagtactatc ggataggagt ctccatattt
     3241 aaaccttagt aaaggtagtt aaagtagtag tttaaaaact aaaaaattat ataaaggaat
     3301 taggtataag aaaatgattg agagtatacc agcaaaacaa attttacaaa aagtgaaatt
     3361 tgacaataca agatggtttg gaattgacta taatatgaat ctctatcgtg gttgttctca
     3421 tggatgtata tattgtgata gccgtagcac aatatacaac atagagaatt ttgacaaagt
     3481 tagatataaa gaaaatgtga tagaaatttt aagtaaagaa ttgcgttcaa aaagaaaaaa
     3541 aggtgtagta ggcattggag ctatgtctga cacatataat ccttttgaaa agcaactttg
     3601 tattacaaag caagcactag atttgatttc ggaaaatcat tttggagtgt ctattgatac
     3661 taaaagtagt ttggtggtta gagatatacc gattttgcag aaaattaaaa aaaataattc
     3721 tgctattgta aagttgacta taaccactgc aaatgatgaa ttatcaaaga aaatagaacc
     3781 ttatgtaaat ccaagttctg tacgatttga cgcagtgaaa gaacttaatg attctggaat
     3841 tttttgtggc atattattga ctcctatgct tccatttttg actgataata aagatgaaat
     3901 acgagcaatt gtagaaaaag cacacaaggc taatgcaaaa tttatttatt gcatgtatgg
     3961 tgtaacaatg cgtagtggtc agagagagtt tttttatgag catttaagag atatatcacc
     4021 aaaattggta cttaaatatc agaaaacata tggtttaaac tatgtatgta ctgtacaaaa
     4081 taaagattct tgtgagaagt tgttaagaga agaatgtgca cgttatggta ttttgacgga
     4141 tatgaaagat attataaaag cgtacaagag gtcggagtca tatactcaaa taaaattttt
     4201 ttaagatagg ctttggatga gatataatag tgttgattga tgtgttgata ttatagttga
     4261 ttgttagaga ggatattata gatgaatatt agtattgata ttttaggtaa ttgttagagc
     4321 tgatattata gatgaatatt agtattgata ttctaggtaa ttgctagagt tagtattata
     4381 gatgaatatt agtattgata ctctaggtaa ttgctagagt tagtattata gatgaatatt
     4441 agtattgata ttctaggtaa ttgctagagc tgatattata atttattatt agtattaata
     4501 ttctagggaa ttatcactat attttaaaaa gtaaaaatat atatcaaaat gaattaaata
     4561 agtactgtaa gaagtttgtt aaattaaggt ttgaataaag tgagggtgga gaacttatga
     4621 tagaaataaa aactatagaa ataaataaaa aaagtcgaat tgacctttta atgttagccg
     4681 acccatgtga agaaatgatt gataaatatc tcaataaagg aactatgtac gcattgtatg
     4741 ataataaaga acttacttgt atagcagttg taaatgaaat ttccaaagaa atatgtgaat
     4801 taaaaaacat tgctacatat gagcattttc aaaatatggg ttatgcctca aagatgattt
     4861 atcatctttt agatgtttat agtaaaaaat attcatcaat ggttgttggt acatctgaaa
     4921 gcggagttcc tttttatgag aagtttggat ttgtttattc acataaaatt aaaaactttt
     4981 ttgtagataa ttatccagaa ccaatctttg aaggagaatt acaatgtgtt gacatgttgt
     5041 atctatcata taacttttag ataggataat atttttaggt taatataatt aattaattca
     5101 tataattatc ggaattataa tttataaatt atgtataata agagtataat ttatttagaa
     5161 gctgttatat tgtattatat tcaaataaga tatataataa cttgtttatt ttgaattttg
     5221 gaggattaat tatggattca aataataata ctataaaatc aactgttaaa aagggtattt
     5281 cttttggttc ttgtttagca atgattattt cttatactgc atggaaatct attccatggg
     5341 ctatttttca tggcttaatg agttggatat atgtacttta ttattgggtt aagtatgcat
     5401 agattaaatt aataaataag tatgtaatat tgtaaagttt aagaaacaaa atgtaaggga
     5461 gtgtctcgaa gtgaactttt agtttataag gcactctttt ttgtattaaa taaaatatat
     5521 tttcattatt taaacaatga aaaagatgag tatctctatt ttaatacata cccttatatt
     5581 cattcattta tcaacaatag ttttaaaaag aaagctatct aaaatctatt gtaatctaag
     5641 tgcttctctt taaagtctta actttagtaa tgatatataa gtttataagg ttcttatttc
     5701 aataatgcaa attttactta gaagttcaga ctttggtatg aagaaatggg taataataaa
     5761 aaagaggtgt tgatatgaaa gaaaaattag aagaaaaatc taagattatt aaaacatcaa
     5821 aagagtataa acgaactata attgaagaaa aggacagaga atggaccaaa aaattaaatg
     5881 acatacttag tagaggtgat ttttctaatg aaaaattaga taaaatcaag gatttaattc
     5941 caaaagaaat attgactagt gaaaatgttg gagaagttgt aactgaagat gggtatgcta
     6001 agcctgagta tgatgaggta tttaaatcat tgtttacttt aaataatgat tacgatttac
     6061 ttgcaaattt tataaatgat atcctcaaag atgctccata tgctaatagg aatatcaaac
     6121 agttcactca aataaaaaga ataattaagg ttgaaaccga cccaacaatt aattacattg
     6181 gtgagaaaag acctagactt gatattttgg ctgaagatga agaacgtaac catataaata
     6241 ttgagatgca aagagctctt gaagaggatt atttggaaag agctgaatat tatcttagtc
     6301 gtgtacatgg tagaaagtta gaagaaggta aagaatataa agaaattgga aaaactgttg
     6361 gaatacatat actaaatcat gtaaaatata atcacataga agattatgta aattgcctga
     6421 gattaacaat ggacgggcat ccagatattt attctagtaa aactgcatta tattttatag
     6481 aacttccaaa aattcgtaaa tccagttgta tagcaaacag agttctaata tggggtaaac
     6541 ttattgataa tccttctcat atagatatta gaatactatc taaaacggat tatgttatta
     6601 aaagagcact tgatagatta aaagaattag gtagtaatga ggactattta ttaaatttaa
     6661 agagaggtgc gtatattatg aataaaagta gaaattttaa agaagaaatt tttcaagaag
     6721 gtattgaaaa aggtattgca aaagggaaaa gagaagagaa atttaatatc atcataaaat
     6781 taaaaaataa aggatacaat ttgagtgaaa tatgcgatat tatagatgat ttgaataaat
     6841 cagaggttga aaaagtctat aatcaaaact aatactatga ttgagatggc actagataaa
     6901 tacaaagctt cctcttttaa atttaaatta atataaaact ttgttattta aatatggtag
     6961 atataaagag atatataaat aataggtaca aagaggtgta taaataaagg agataattca
     7021 aaataaaagt ttgaaccatc tccttcttat agaaaaacaa aggggttgat ttatatatga
     7081 aattttatac tatacagact ttagaatttt ggaataacaa taaaaacaat aaatatctat
     7141 caaatgatta ttcttttata ttagaagact ttgatattcc atacaaatgg atgtatagtc
     7201 aaatgacaaa tagaatatat gaatttgatg atagtatgat atgggtttgg acgaagagac
     7261 ctgatttaag atgtagtggt tatttagaaa aaggaaaaaa aggtgtttta cttgaaatag
     7321 aaataagtga agacaatgtg ttattatcag aatttgattt atggcattta cctttaatgg
     7381 atattactgt agagctttat gaagatgaac aaattgaaaa gaaggtaagc tgggaaagaa
     7441 tatttgactt agaactgtgt tcaaaattaa atgaagttaa tatagaagat ttgacattgc
     7501 aaggagtgac atctaaagtg cctctatcta atgtaaaatt aataaaagaa tttgttgcaa
     7561 aataatattt ttattgaaat ttattcaaaa aagttatata tttataacaa gaggtgttat
     7621 aaatacataa ctttttaagg gggattttat atgtctaaac aacaaactgg tattaaatca
     7681 acacttattt cagaaatatt gggaatttct tattgtattg gagctacatt agtaaataag
     7741 tattttttta aaggaagtag tattgaatat cttcctataa ttatttgggt attagctatg
     7801 ggagctcagt gggtctataa taaaaagtat gatgatttaa ttgatgaggc atcaaaatgt
     7861 atactatcaa gagttaatga tattgcaata aaagtattgt ttttttctgt agctatagta
     7921 tctatatttc ttgtaacttt ggacataaat gtgtcaaatt taaatatagg aatgtcttta
     7981 ttagtcattt tgtttattca atccttgttg aagttaattc tatttatata ttttgacaga
     8041 aaggggatat acaattaggt gtcagtatta ctaaagacaa aaattaaatc ctatagagaa
     8101 aagaacctta tgacacaagg tgaacttgca aagtcagtag gagtaagacg tgaaactatt
     8161 gtacatttag aaaatggtcg ctacaatcct tctttaaaac tggcaatgga tattgctaag
     8221 gtatttgata caactgtaga agagttgttt gaatttattg aagaaaataa tatttaaatt
     8281 tcatgtatta cttatgcatt agagaatgaa ttaattatag gtaaaaaacc aagcttatag
     8341 attagagaat aaatgactta tagatgaaaa accaagctca tagattaaag aatgaatgat
     8401 ttatagatga aaaagaaagc ttatagatta gagaataaat gatttatagg taaaaaccaa
     8461 gctaatagat taaaaaatga atgatttata gattaagaag taag
//
LOCUS       DAYATN010000148         8475 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.149, whole genome shotgun sequence.
ACCESSION   DAYATN010000148 DAYATN010000000
VERSION     DAYATN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.149"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(222..1265)
                     /locus_tag="ACGI8A_003196"
     CDS             complement(222..1265)
                     /locus_tag="ACGI8A_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFH0181175.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(1286..1732)
                     /locus_tag="ACGI8A_003197"
     CDS             complement(1286..1732)
                     /locus_tag="ACGI8A_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFH0181176.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(1906..3486)
                     /locus_tag="ACGI8A_003198"
     CDS             complement(1906..3486)
                     /locus_tag="ACGI8A_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFH0181177.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            4285..5187
                     /locus_tag="ACGI8A_003199"
     CDS             4285..5187
                     /locus_tag="ACGI8A_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181178.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            5174..5929
                     /locus_tag="ACGI8A_003200"
     CDS             5174..5929
                     /locus_tag="ACGI8A_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181179.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            6026..7063
                     /locus_tag="ACGI8A_003201"
     CDS             6026..7063
                     /locus_tag="ACGI8A_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0181180.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            7872..>8475
                     /locus_tag="ACGI8A_003202"
     CDS             7872..>8475
                     /locus_tag="ACGI8A_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181181.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNM"
BASE COUNT         2901 a         1315 c         1050 g         3209 t
ORIGIN      
        1 tatatgtttt atataatatt atataacact ttttacatta gtaaaggata aattgtataa
       61 aaattctgtt aatttatttt tataatttca tctttctaat aaaaacaaag aagaagactt
      121 aaaaatttat aagtcctctt cttttcaacg tgcatttttg tgctatattt aattaattcg
      181 acctttaatt tccatatcct tcctatgata acaaaactct tttatttttt taatgattca
      241 ttatcattta aaacttcttt tatatcaagt acttctttgt tatcatcact cataacttcc
      301 tttattcctt tgaatgttcc taggaaatta actgcttctg aaggtaaaac cagttttgtt
      361 gacttacctt cagccatttt ttcaagagct tccatagatt ttaatgctag aatattattg
      421 tctatatcag cctctttcat tgctgaaaaa actaatttta aaccttctgc tgttgccatt
      481 tgagttcttt ttatcatttc agcttcacct tgtgccttag caatagctgt ctgtcttata
      541 gcttcagctt caccttctgc cactaatata gcagattctt tttcaccttc tgctacacgt
      601 accatagctt cttttttggc ttctgctgtt aatatagcag attgtttttc accttcagct
      661 tgaagtattg cagctgactt attaccttct gcttgaagta ttgcctctct tctttctctt
      721 tctgctctca tttgtttttc cattgcaact tgaatatcct gaggaggcat tatatttttt
      781 aactctactc tatttacttt tataccccat ttatctgttg cttcatcaag aatagttctc
      841 atttttacat ttattatatc tcttgatgtt aatgtttcat ctaaatcaag ttcaccaatt
      901 atatttctta gtgttgtagc tgttaaattt tcaatagcag ctattggatt agctatctca
      961 aacacatatc taactgggtc tgtaacttta taatatacaa ctgtatcaat ctgcatagtt
     1021 acattatctt tagttataac tggttgaggt ggaaaatcta ttacaatttc tctaaggtca
     1081 attacatatg ccattttatc taaaaatggt attaagaaat gtacacctgt ttcagccact
     1141 ttttgaaact taccaagtct cattattata cctactttag attgttttat aactcttata
     1201 caagttaaac ttattgctac gactactact attaatacaa tacttaaaac aattttagta
     1261 cccatactat aaatcctcct ttattctatt ggtttgtcac attaactgtg gcgtctactt
     1321 ttttaacaac taattttacc ccttcaattt ttaatacttc tactgtagaa cctttttcta
     1381 tcttttcacc atctatagaa atagccgtcc atgtttcatg ttcaactaca actagacctg
     1441 tgttatttgg agaaatctct tctgtaacaa tacctttttt agacatcatt gcttgtaaat
     1501 tagtgttaga tttatagcct ttatcttttt ttactatttt tttagtagct acgactaaca
     1561 ttgctataga aattactgca aatattaaaa tttgtaagaa aatactctct ataaagctac
     1621 ttaaaaatat aagtattaca gctcctacac taaaccatat caatgttaag cttggtgtta
     1681 acatttctgc tattccaaat aagactgcta ctatcaacca tattagcttc atactatcta
     1741 ccctcttttc atatgaatta taaaacccta tataaattat atgtttatac aatgtttttg
     1801 aatataaatt taaattacaa aacaataaaa taaattaatt aattaatagt attatatcaa
     1861 catactttgt taaaattgtt acaaagtagt atcaaatatc accaattata tatccaacaa
     1921 ctctttcatt gttaatgaaa tgttacttat taaatccgta gcatttaccg tatacattgc
     1981 tttgctcaat ttatctccta tatagccatg tatatatgct ccactaactg tagcttccaa
     2041 tatatccata ccttgaccta taaatgaagt aatcattcca agcaaacaat cacccattcc
     2101 accagttgcc atacagctat ttcctgtagg atttatatat gtttttttac catctgttat
     2161 gacagtttga taacctttta aaagcaccac cagattatat ttttgagcaa attcttttgc
     2221 aacatctact ctatgttctc ttatataccc aattgaatct cctgtcaacc tggccatttc
     2281 tcccaaatga ggtgtaatta caaatctatt tttccattct aaaaacttat aacatctgtc
     2341 cttcataacg ttcaatccat ctgcatctaa tactattggg caattagaat tttctactat
     2401 cttaagtaat ttatcaaatg tttgactatt atctcccata cctggaccaa aaccaattgc
     2461 atcactagaa ttaagtaatt tatttaatct ttcttcttca aaatttaaag tcattgcttc
     2521 tgtaagtcta atagatacct tttcttgcac atcttcacta ctaattaatg ttacaagtcc
     2581 actcccactc ttaacagcag attcagttgc tatgaaactt gcaccataaa atccttttga
     2641 acctgcaact atagatactt ttccaaaatc acttttaaaa ccatgcttat ctcttcttct
     2701 aatattattt tttacaaaag tcttatcagt tatgaactct tcatcatggt attttcttgt
     2761 aataaaatct ggaacaccta tcttttctac aactacatca cctataagtg cgctagtgtc
     2821 atagtttaga aaacctcttt tataaaactc aaagctaatc gttttgtttg ctctaataca
     2881 agttcctaat acatttccat tatcgctatc tatacctgaa ggaatatcta tagaatacgt
     2941 atttttgcta ttatcgttta ctatgtcaat aacttcttta aaaataccag ttatatcacg
     3001 ttttaatcca gtcccaaata tagcatctac tacaacatca cttttagata ctctatcttt
     3061 aaaaattgtt aaatttttat tttttataat attttgtttc tcaactttaa taattttatc
     3121 tacttcattt tcctctccat aatttttttc acttaaatca tttttattaa tatttataat
     3181 attaattccc atatttaaaa gtacattgta atttatttta gagcattcac ttagtttttc
     3241 aagattacct actaaaaata cgtttacttc ttttccatat gtatttagat gtcttgctat
     3301 tccaagacca tctcctccat tatttccaac accacataca actgtataac tattatacat
     3361 gtctatatcc atatttttca ttgcagatat aaccgcattt tccatcaata caatcattgg
     3421 aatccctact tcttctacac atgcagtatc aacatttctg cttaatttag atgtacctat
     3481 tagcataaat tatcactcct ataaagttaa agacttctta aatacttacc aaaaatatct
     3541 attgaaatct ttttaatccc ataaatatca taaataaaat cactaaaact aagagttttg
     3601 aaataattta aaataacaaa aaattttgaa ttaaaacaat tatttaattt attataccat
     3661 ataatttaag ttattatgtt gatttaaaac ctatttacac taattatcta aatatctctt
     3721 tatttttata atcttttata acttatttat atttttcatt tttataaaaa aacaataatt
     3781 aagaatataa ctatattcta catgtaaaaa tttttttatt taaactcaat tttctgataa
     3841 tttgataatt tttcaaaatt tatagttgac ttttattttt tactgtatta aactaattac
     3901 tatacaaaac agaaaacaaa attttcaaaa ttattaaatt aaaaggagag gtaaatatga
     3961 agactttatt aaacaaacaa gtacgatttt ttagtagcca atttagttag ggtttgtacc
     4021 taagttaaga caaattgtca taacatagat acaagcccct taggattatg atttttaagg
     4081 attgtatcta tgttggctat agtaattgtt gttaaagttt ttattttatc atcataatat
     4141 agagccacat ggattatact gtgtggctta ttcgaatttt tgccatacag ttatatatac
     4201 tgtgtggttt ttttatatca aaatcatttt ttaaaaccaa attttaaaaa ccatttaaaa
     4261 tattttcaaa ggggtgtttt atttatgtca ggtattatat ctgtaatgac gcaaagttta
     4321 attttatcga ttatggcact gggggtttat ataacttaca aaatcttaga ttttccagat
     4381 atgtctgctg atggtagcta tacaatgggt gcttctattg tagcattttc tcttactaat
     4441 ggtatttctc ctgtagtagc aactttaatg gctatcttat gtggttgtac tgctggtctt
     4501 gttacaggga ttcttcacat taagtttaag atatctaatt tactttctgg tattttagtt
     4561 atgggaatgc tatactctat caatttaaga ataatgggaa aatcaaatat tcctctattt
     4621 agctttaaac acttattcaa tggggaaata tctcctatag ttttagcctt agcttttgta
     4681 ttcatctgta aagtcctttt agatttattc ctaaaaacag gtcttggata cactttaaaa
     4741 ggtgttggtg ataactctca aatgattaaa tctctaggta taaatatagg ttctataaaa
     4801 atactaggac ttatgatttc aaatggactt atagctttat ctggtagtct tatggctcag
     4861 ttcttgggat tctcagatgt aaatatgggt ataggaactt tagttcttgg aattgcctct
     4921 atcataattg gtattacact atttaagaaa tttactttta tcaaagatac tactgcaata
     4981 attgttggct cttttatata ccaattcact atatactttg caatgagttt aggaatgtta
     5041 tcaactgact taaaactaat aactgccata gttataatag ctttcctagc tactggtaac
     5101 ttaaatattt cactaaaaaa aaacaaatgc aaagctagta ccaaaaataa atcagaaaaa
     5161 agaggtgttg tagatgttac aaattaaaaa tctttcaaag agtttcccta atccatatgg
     5221 tgaacctaat actatctttg aaaatttatc aatcgatata gaagatggtg aatttgtaag
     5281 tatcataggt agtaatggaa ctggtaaatc tactttatta aatataatat ctgggcttat
     5341 aaaagaatct tctggtcaaa ttactttaga tagtactact ataacaaatt tagctgagta
     5401 taaaagaact caaatagtaa gtagagtttt tcaagaccca agccttggaa cttgtccttc
     5461 tatgacggtt agagaaaatt tatctctagc acttaataaa gggaaattat tgaacttaaa
     5521 aaaatgtctt cgtcataaaa cgaatgactt ggaacactta cttgaaggaa tttctttgga
     5581 tttaaaaaaa tatttagata ttaaagtaca atatctttca gggggtcaaa gacaatcact
     5641 atcactaatt atgtctagct tagcaagtcc taaggtctta ttactagacg aacatacagc
     5701 agcacttgac ccaaaaacat caaatgaagt aatagagtta acagataaaa tcgtaagaga
     5761 gaaaaatata actacactta tggttactca caacttgaaa cacgctcttc aatatggaga
     5821 taggttgata atgcttcata agggtgaagt tgtattagat gtaaaagata aagaaaaaaa
     5881 agatttaaca gttgaagaaa tcttagaaaa atttgaatat gcagtataag aaattaaaat
     5941 cttagtttta atcagaaata tttataatct atcatatgaa tatatgaaca aatataatta
     6001 ttaaatttta agggaggaaa ataaaatgat aaaaagtaaa aaaatattaa gtttaattat
     6061 agcaggagta cttggtgtat caatgcttac tggatgttct caaaatgatg gctccaatgc
     6121 ttcaaatgaa aataaagaaa cagatagcaa aaaacaaaaa aatataggta ttactcaatt
     6181 agtggaacat ccatctctgg ataaagcaaa gaaaggattc atcaaagcac ttgaagataa
     6241 aggctataaa gatggagata atataaaaat agatttccaa aatgcacaaa atgatatgcc
     6301 tactacacaa agtattgcta gtaagtttgt atccgacaaa aaagatttga tatatgctat
     6361 atctactcca tctgcacaag cagcttataa tgctactaaa gatattccaa taataatgac
     6421 tgctgttaca gaccctgtag aagctggact tgttaaatct cttgaaaaac caggtggaaa
     6481 tgtttctggt acatctgatt atctttcaat tgataaaaca ttagaattgg ttaaaacatt
     6541 gactccaaaa gcaaagaaaa taggggttat atacaatact agtgaagtta attcaaaaat
     6601 ccaagttgat tctctacatg attatgctaa gaaaaataat tatgaagtag ttgaaaaagg
     6661 aatcagctct tcaagtgaag ttaaccaagc tatttctagt ttagttggta aaatagatgt
     6721 tttatatgtc cctactgaca atttaatagt ttcttctatg ccaatagttt ctaaagttgc
     6781 taatgaaaac aagattccta taatagcctc tgaagaaggt tctgtatctt ctggtgcttt
     6841 agcttgttgt ggaatagatt atgaaaaact aggttataag gctggagaac ttgctattga
     6901 agtattagaa ggaaaatctg ttggcgatat accagttact acattagatg aaactgaaat
     6961 aataattaac gaagatacac taaaagcact tgacatgcaa aagttatcag cagataatat
     7021 aaagtatata aagtcagatg aaaatgcaaa atctacaaaa taaaacttat taatttcaaa
     7081 aaataggtaa tatgaagtag gtaatatgaa aactttatat aataagtact cttatccacc
     7141 ctaacccttt gaaaattgtg cttattgtag caatttgaat attgcatata aagttattca
     7201 ttttccataa aagtcgtcta tattgacgac tgattatgaa agttatataa ctttctaatc
     7261 actacttgag ctgtccaaaa gaattttaat attctccaga cagctttttt ttatgatatt
     7321 tgattgatat acatgtctag ttattccata tttagtaaat attttgataa aatttccaca
     7381 ttcacttata acttaggata acgttttttt caaacaatac attaatttaa aagtattctg
     7441 agtatacatt atttagtatt ttaattaaat ttagcagttg acttttatta ttgtataatt
     7501 tataatttat aacaagtcga gaatacatat aattttctga ctaattaaaa tattaaaagg
     7561 gagagataat tatgaatact ttattaaaca atagaaaata ttattattat tatagccact
     7621 atttttaggg tttgtaccta cgttttaaca tagatacaga ccccttaaga ctactaaaat
     7681 tttgggattg tatctatgtt ggctatattg tttataaagt tttcatctaa ctatataaga
     7741 gccacataga ttaaactatg tggcttattc gtgtttttac cacatagttt tgtacactat
     7801 gtggtttttt tatacacaaa attagcaaat aattaaaatt ataaaaatat tttcaaagga
     7861 gtgttttatt tatgtcaggt attatatctg taatgacgca aagtttaatt ttatcgatta
     7921 tggcactggg ggtttatata acttacaaaa tcttagattt tccagatatg tctgctgatg
     7981 gtagctacac aatgggtgct tctattgtag cattttctct tactaatggt atttctcctg
     8041 tagtagcaac tttaatggct atcttatgtg gttgtactgc tggtcttgtt acagggattc
     8101 ttcacattaa gtttaagata tctaacttac tttctggtat tttagttatg ggaatgctat
     8161 actctatcaa tttaagaata atgggaaaat caaatattcc tctatttagc tttaaacact
     8221 tattcaatgg ggaaatatct cctatagttt tagccttagc ttttgtattc atctgtaaag
     8281 tccttttaga tttattccta aaaacaggtc ttggatacac tttaaaaggt gttggtgata
     8341 actctcaaat gattaaatcc ctaggtataa atatcggttc tataaaaata ctaggactta
     8401 tgatttcaaa cggacttata gctttatctg gtagtcttat ggctcagttc ttgggattct
     8461 cagatgtaaa tatgg
//
LOCUS       DAYATN010000149         8468 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.150, whole genome shotgun sequence.
ACCESSION   DAYATN010000149 DAYATN010000000
VERSION     DAYATN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.150"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            118..348
                     /locus_tag="ACGI8A_003203"
     CDS             118..348
                     /locus_tag="ACGI8A_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HFH0181182.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            361..510
                     /locus_tag="ACGI8A_003204"
     CDS             361..510
                     /locus_tag="ACGI8A_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HFH0181183.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            767..1204
                     /locus_tag="ACGI8A_003205"
     CDS             767..1204
                     /locus_tag="ACGI8A_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFH0181184.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSIPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            1530..2354
                     /locus_tag="ACGI8A_003206"
     CDS             1530..2354
                     /locus_tag="ACGI8A_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFH0181185.1"
                     /translation="MNWIFSLTPALLGITNTILGEIIFYLAYTSPAIVGLFFISTIYP
                     QSAKQDFIYRYLSFKEIGIKWILLTILFFALTFITSLIIGNYFNVKTSDIDWIHIAVF
                     RPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVRFGFIGSSIILGLVCGIWYLGSYF
                     TPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVYINTSRSILSAGLVHMMYYFFNIQ
                     LLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFKQKVNSEIEQIKSDDFEFELDW"
     gene            2532..3356
                     /locus_tag="ACGI8A_003207"
     CDS             2532..3356
                     /locus_tag="ACGI8A_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0181186.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            3482..4063
                     /locus_tag="ACGI8A_003208"
     CDS             3482..4063
                     /locus_tag="ACGI8A_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0181187.1"
                     /translation="MKADSTKEQLINATIQLLSSHRDVSSITAREIVAKANVNLAMIN
                     YYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFDFIGEHSQFI
                     KISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSFIQLLFLRSN
                     AFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            4167..4343
                     /locus_tag="ACGI8A_003209"
     CDS             4167..4343
                     /locus_tag="ACGI8A_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181188.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            5609..6361
                     /locus_tag="ACGI8A_003210"
     CDS             5609..6361
                     /locus_tag="ACGI8A_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181189.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            6351..8462
                     /locus_tag="ACGI8A_003211"
     CDS             6351..8462
                     /locus_tag="ACGI8A_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181190.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         3210 a         1086 c         1116 g         3056 t
ORIGIN      
        1 taatattgac aattgaaata ttaatactaa caagcaaaac attaatattg ataattaaaa
       61 taccaatatt aacaagcaaa caattaaaat ataaattatt atatcggagg taattatatg
      121 gaaattaaga aaaaattagt catgagcttt aaaaatgtaa aggataaaca agtttctttt
      181 tctatagaaa atccaaagga tgatttaact gaagaaaata taaaatctgt catggatttg
      241 atagtatcta aaaatatttt ttcggttgga ggatttgacc tagcttctgt tgttgaagcc
      301 aaggttgtgg aaacaaatac cactccttat gacttagtta tagaataggg ggctaatttt
      361 atggatgata gtttacaact gttaataacc aatgttggtt ttcctatagc tctatccatg
      421 tacttgttgg ttagaattga gggtaaatta aatactttat caaacagtat tgatacatta
      481 tccaaaaata ttttgatatt taagaagtaa tttgacaata tatgtaagtc ataaaatagg
      541 tctatttgta aaataattag acatattcta tgtgaaatac taggatattt ggataattta
      601 tccaaatatc catagtattt ttttgaattt taccacctca aatttgaatt tcactgtttt
      661 atttttgaat ttcactatgc ttaatttgaa atttagaacg catctagttg gactcaataa
      721 gatataatgc tattctaatg acagtaataa aatgaggtga tgattaatga aaatacatgt
      781 cgaacaaggt attaattgtg atgatattga aataaccata aaatgttctg ctctagataa
      841 taacattgaa aaaataattt cattattgag tataccgtta cttgatataa aaggtaaaaa
      901 atcaggagag atttataaac tcaatgtctg tgatatttta tactttgaga cagttggaaa
      961 taaaacattt gcttattatg aagatgatgt atttgaaatt gatttaaaaa tatataaatt
     1021 atcaaaatta ttagctaata catctttctc acgaatatct aaatccatga ttttaaatat
     1081 ggactacata aaaagtataa aagtaatatc acataatcgc atgcaagcta ctttaaaaaa
     1141 caacgaaaaa gttattataa atcgtcgtta tataaaaaat attaaagaaa agctaaacat
     1201 ttaataaaaa tctaaagtaa tttattaata tcactaattg caaatttatt gacctattta
     1261 ataattaata ataatataat atattcaata ttaaatacta attaattata tttagtgtaa
     1321 aaataattaa tcaaaatata aaaaatagga tattattata ggcaataact aacatcaagc
     1381 gataacttaa tacatataaa aataaataaa caattaataa aaattaaaac ttcattataa
     1441 aacggaagga tagtgaaaaa atgcaactat ataacacccc ctttaataca aacaaacact
     1501 taatctattt ctttacattt actctagtat tgaactggat attcagtctt acacctgcgt
     1561 tgctcggaat tacaaataca atacttggag aaatcatttt ttatcttgca tatacatcac
     1621 cagctattgt aggtctattt tttatttcta caatctatcc acaaagtgca aaacaagatt
     1681 ttatttatag atacctaagc tttaaagaaa tcggaataaa atggatacta ttaacaatat
     1741 tattttttgc tttaactttt attacttcgt taataatcgg aaattacttt aatgtaaaaa
     1801 cttctgatat agattggata cacatagcag tgtttagacc ttctaaaata gcttatatgc
     1861 tattagcaag ttttatattg agtatactta gtcaagaagc tggatggaga ggatatgcca
     1921 ttgacaaatt attagttaga tttggattta taggctcttc aattatttta gggcttgttt
     1981 gtggaatctg gtatctaggg tcgtatttta cacctaatca aataccatat aatttggcac
     2041 aatactccct atttgatgct tttttattta taccaaacat tgtactttta aactttataa
     2101 ttaattttgt atatatcaat acaagtagaa gcattttatc agctggatta gttcatatga
     2161 tgtactattt tttcaacatt caattacttc tacactaccc tattaaatta tgtgtaatag
     2221 ctcaatatgt acaaataata tttggtttaa tatttcttgt atatatgatt tcttctaata
     2281 aatttaaaca gaaggtaaat tctgaaattg aacagataaa gtcggatgat tttgaatttg
     2341 agttagactg gtaattttaa tatgaattat aaattaagat aatctatgat ttgatacgat
     2401 tactacttaa atataattta ctctctataa tttattgcaa catctggaaa ataattgtat
     2461 ttccacaaac gcttgaagta tataaaaaca aaatatatac tgattataag tgaaaaggag
     2521 gtcatatttt catgtttaaa ataggagagg tttcaaaact aacacaaatt tctattcgta
     2581 tgttgcgtta ttatgatgaa cttggtattt taaaacctgc aaaaacagac aaatatacag
     2641 gtcatagatt atactcagtt gaacagattt caatattaca aagaattgta ttattgagag
     2701 attcaaaatt tagcgttgca gaaatagcaa atatcgtaca taattggaat gatgaatttg
     2761 taattaagga attgaataga aaaaagaatg aaatacaaaa ggaaataaag caggaacaac
     2821 aaagaataaa taaaattgat aaatttatag aagctataaa ctgtgataaa gatgaaattc
     2881 attacaatgt tgtctttaaa aagattccaa gttataaaat catatcactt agagagataa
     2941 ttccagacta ccaatctgaa ggtatactct gggaaaagct atctaaattt atcaaagaag
     3001 aacatataga agtatcacgt caatccaaca ataacatagc tttttaccat gatgaagaag
     3061 tcaaagataa tggtgtagat attgaagttg gaatggttgt aaagaagatt ggaaagaaca
     3121 aatctggatt tatatatcgt gagacagaag aaattgatat gatggcatgt actatggttt
     3181 atggtccata tgaaaatata gctggtgcat atgaatcgtt ctgttattgg ttagataaaa
     3241 atagtgatta tcaaatatca ggtataaata gacaaatcgg acataaaggt gaacataatg
     3301 aaataaatcc tgaaaattat cttacagaaa ttcaaatccc ccttataaaa gtctaataga
     3361 ttagtactta aatacttaag tactaatcac ccattgacat cccaaaattt tcatgatagt
     3421 atctaaacgt gtattaaaca tgtttaattt ttttatggag aaacttttaa ggggaaaaat
     3481 tatgaaagca gattcaacaa aggaacaatt aattaatgcc acaatccagt tactatcttc
     3541 acatagagat gtttcaagca ttacggctag agaaattgta gctaaagcaa atgtcaattt
     3601 agctatgatt aattattatt ttaaatctaa agaggagtta attaatattt ctattaaaaa
     3661 atttttaaag cgtcatatgg atgaatataa ggctacacat gatactaaaa atttagcacc
     3721 taaacagcaa ttaagggaaa tactaattgc attttttgat tttataggtg aacatagtca
     3781 atttataaag atttctgtac cctatatatt gatacaggaa gaaattgttt atccatttga
     3841 gatactacct ttaattaagg ctcactatga aaatcagaat aacaaaaaaa ctgaagctga
     3901 atacagaact attgcgtatc aaataacttc atttattcag ttgctttttc ttcgttctaa
     3961 tgcttttttc aagtatagtg gtattgatat tcttaatatt gaacagagaa atggtttaat
     4021 agattttcaa ttagatttat tattggtaga tattacaaaa taatactata ttaatttttt
     4081 atgtacattt ttttatttta tggtaacgtt tgaaaatatt ctcattgact aaaaatactt
     4141 gcattaaata agggggagga attttaatgt tttttacatt tataggagta atatcagcaa
     4201 ttttattgtt gtgtacaatt atatgtggtt tatggataaa acttaataat cgtaataatg
     4261 actctaattc tcactttact ttttcaatac tttcaattgc tttttcactg ttaattgtgg
     4321 tggccttttt agtaagaaat taatgtcttt gttatgctga ggtttattat tatagatttt
     4381 tattattgca tgatttttac tatattatag tatttttatt atattacagg gtcttattta
     4441 ttatagtgtt ttgttgttac agcattttat tatattacag agtcctattt attataggat
     4501 ttttattata ttgtaaaatc ttatttatta taggattttg ttatattaca gacttttatt
     4561 atattgaaat ctcttattta ttgcagtgtt tctattatat tgcaaactct tatttattat
     4621 ggtgttttgt tattgcaaca tttctattat attgtaaagt cttatttatt acagttcttt
     4681 attatactac agacctttat tattacagca tttctattac attgtaaagt cttatttatt
     4741 acagttcttt attacactac agacttttat tattacaatc tcttatttat tacagttctt
     4801 tgctatacta cagaccttta ttattacagc atttctatta cattgcaaag tcttatttat
     4861 tacagttctt tattatacta cagactttta ttattacagc atttctatta cattgcaaag
     4921 tcttatttat tacagttctt tattatacta caaaccttta ttattacaac attttattat
     4981 tttaacttct ttactataat atagtaatat tttaatatct caaaatcata tcaatcactt
     5041 ttcagcatag tctaaaatac ttccactttc taaaattatc tatcatacct atactaataa
     5101 tactttttaa cctttttatt atacatttca attgtttcta acaacctatt tatttccaaa
     5161 actccaatat attttctcaa ttacattgtg ttaatagccc ctaaatcctt tcaaattatt
     5221 tacaatacat tccattaaat aatcttactg ctctataatt ctttttttct ttttaaccct
     5281 actatacatt atttacatca tttttttcaa tacaacattc gttattctat aaccttttta
     5341 cttttactat atacttcttt tctaattaca ccttcaatgt tccacatcat attactttag
     5401 cacaacaatt aatacctaaa aataaccctc taaatcctcc aataatataa tcaatttaac
     5461 tataataata acctctcaca ctatccaaac aatatatttt caaacattac aaaaaaacat
     5521 ccaactatta caatatagct catatccctc ctcccctttc aaatgctatc attgacttac
     5581 aagataaatg aaagtgagga tgatatatat gcttaaagta aaccaattat gtaaatcata
     5641 taaaacaggc agtaaaacat atcctgtatt aaaaaatatt tcttttgaaa tcaacaaagg
     5701 agaatttgtc gcaatcatgg gttcatcagg aagtggaaaa actactcttt taaattgtat
     5761 ttcatgtttt atcccaattg ataatgggga aataattctt ggaaataata atataagaaa
     5821 tttaaaagaa aaggaacttt cagaagttag aaatcaacaa ctaggatttg tattccaaga
     5881 cttcatgcta tttgatggtc ttagtgtctt tgaaaatata tgtgttccac aaattatcaa
     5941 aggtagtcaa gtcttacaaa tggaaaaccg tgcaattaat ctatgtgagc tatttggtat
     6001 tgaacatatc agaaaaaaat accctgccga aatatctggt ggagaaaaac agcgtacagc
     6061 agtagcaaga gcactcatga acaacccaga tttaatatta gcagatgaac caactggtaa
     6121 ccttgatagt aagtcttgtc gtacagtcat tgaatcattt ttaaaagcaa agcaagaatt
     6181 agatgctact atactcatgg ttacacacga tagctttgca gcaagcttct gtgaccgagt
     6241 tattgtgctt aaagatggaa taatttataa agaagttgta aaatcaaatt caagacataa
     6301 attcctagat gaattactag aagttttaaa agaaatggga ggcgctcaag atgacaatta
     6361 ataaattata cagtaaactt cgagcctata attggaagaa ctatacaact ttattctttt
     6421 gtataatact ttctgtggta ttagtaactt catatgctct aatttatttt agcccaacaa
     6481 tacaaaacat acttccaact ggtggagata gcagaaaaca ggctacaatg atattctcag
     6541 ttgctatatt aggatgcgct gtctttacta catacgcttc ttccctcttt ttaaaattta
     6601 aaagcaagga aattggtatt ttcatggctc taggaacaga taagaagaaa ttaaaaaaac
     6661 ttctattttc agagatattg ttaatttcaa tagtatgctg ttttttaggt cttatattaa
     6721 gtattcctgt atcttttggt atatggaaaa tatttcaatt agtgattatt gataccagag
     6781 aaatgacata tcattttgga actcaaggat tcatatatgg atttatattt agtgtatttg
     6841 tgacattatg catatttata ctttctgtaa aatttattaa tcgtacaaat ataattgata
     6901 tattaaatga ccagagaata tctgaaccaa tacaggatgt aaaggactgg tatggtatat
     6961 taggtataat ttttattata ctcggtattt ttctaggata tctcgtacct tcactaacta
     7021 taaacaaatt aaattataaa ctaccatcta tatggagctt tacttatcta ctctctctta
     7081 ttggaatata tatgattatg acatacatag ttgtacatgc taaaagagga aaacatccag
     7141 agaaatacta taaaaacatc atatcaacta gcatgatgag atttatggga agacaaacag
     7201 taaaaaacat gtgtgttatt tcatttttga ttgcaggagc attatttgct gtattctatg
     7261 ttcctaatat ggttacaggt atctatgata ctcttaaaaa taaccctata gattatactt
     7321 ttacctacaa tcaaagagat aaacaggtat caaaaaaaga tattgaacaa ttagcagata
     7381 aacacaatat aaaaattcaa aactatatgg aaattccttc tatagaatta attgttgatg
     7441 gtgtagaatc aatatatcat aaagatggaa ctatcgaaaa ggtatatatg aaaaaaagct
     7501 cctatgatat gttctttaat tccaaagatt tttccaagct aattggcaaa ccaataacta
     7561 taaaaaatgg tgaatatctt gctgttgtaa ataaaggaaa tgaaaatcaa gattataaat
     7621 ataccaaatt tatatcaagc ccagtcacag aaaaagaaat cccctttaga tatgctggaa
     7681 aaataatatt taatggttct ttttttcaaa atagaagtat gaacgcctat gtccttaatg
     7741 aggaggatta taaatcattt actaaagatt tatccataaa aaattacaat aattctatac
     7801 tatttaatgt agataaagat acctatgctt ttgccaatga cttaaagaaa gaaattataa
     7861 aaagaacatc taaggaaatg ggcatgttca gtggttatga tgaatacgta aaaaaacgtt
     7921 atgaatcaac tggaagaaaa tattttatgg atgaagaata tccaagtggt gaaggtcatt
     7981 taaaactaga cccaaacaac aatcagttgt atcttaattg gaaatactat cctaacttta
     8041 aaattttaca aagccaagat atgataaaaa acatggcagt cttcttaatg ttattcatat
     8101 atgtggcaat catatgtttt acagcagtag gaatcattgc atatacaagg ggtgttacta
     8161 tagctataaa tcacaagcag gtctttatgg atttaaatcg attaggagca aacaatgatt
     8221 atataaagtt ctgtattaga aatcaattaa agaaagtatt ttcataccct cttattgcag
     8281 gaagtttttt aatttatctg tttaatttta tgattatgtt aaataatgat ggtagaatcg
     8341 tcaatagtga ggtcaatgct cttttgataa acttaggttt tatagggatt tgtggggttt
     8401 atatgtacct tatatatcta ttaacttata agaaattcaa aaaaattatt ggtattttat
     8461 aataaaaa
//
LOCUS       DAYATN010000150         8430 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.151, whole genome shotgun sequence.
ACCESSION   DAYATN010000150 DAYATN010000000
VERSION     DAYATN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.151"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(838..2244)
                     /locus_tag="ACGI8A_003212"
     CDS             complement(838..2244)
                     /locus_tag="ACGI8A_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0181191.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(2289..2996)
                     /locus_tag="ACGI8A_003213"
     CDS             complement(2289..2996)
                     /locus_tag="ACGI8A_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0181192.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            3158..4081
                     /locus_tag="ACGI8A_003214"
     CDS             3158..4081
                     /locus_tag="ACGI8A_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181193.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            4081..4842
                     /locus_tag="ACGI8A_003215"
     CDS             4081..4842
                     /locus_tag="ACGI8A_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181194.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4854..5591
                     /locus_tag="ACGI8A_003216"
     CDS             4854..5591
                     /locus_tag="ACGI8A_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181195.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
     gene            6469..6738
                     /locus_tag="ACGI8A_003217"
     CDS             6469..6738
                     /locus_tag="ACGI8A_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFH0181196.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(6956..7201)
                     /gene="cdd1"
                     /locus_tag="ACGI8A_003218"
     CDS             complement(6956..7201)
                     /gene="cdd1"
                     /locus_tag="ACGI8A_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Cdd1"
                     /protein_id="HFH0181197.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            7627..8325
                     /gene="tcdC"
                     /locus_tag="ACGI8A_003219"
     CDS             7627..8325
                     /gene="tcdC"
                     /locus_tag="ACGI8A_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HFH0181198.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT         2981 a         1176 c         1110 g         3163 t
ORIGIN      
        1 acttcccact tcctaaattt cttacttact accacttata actaaacttc ccacttccta
       61 aatttcttac ttactaccac ttatcactaa acttcctact ttctaaattt cttgcttctt
      121 aagaataatg ttaattttaa ttttaagatt atagttttta ccttgtttaa ttgaaacatt
      181 ctatataatt atatccttct attttagagt ttttttattt atatattaca aagtttaaat
      241 tttttcatta attatattat aaattttcca tatatttcaa cataaataca tatatatata
      301 tgttatttct aaaaatacta cacaaaaact ctactttagt agaactcaat ggcgcaaaat
      361 acttcaattt aaaacattta tcgtacttta aataaataac tataataaaa tatacttaca
      421 atagttacat agaagctatt ataactacac agaacagctt ataattaaaa cttttttagg
      481 atgtcattat attttttctt aaaattaaaa aacactatac cataattcta ttttagtaga
      541 atttttttgt caataataaa attaaatatt aactaaaaaa taaaaaaaat ggaattatct
      601 aaagaattta ataaccttac aataattcca aaatattatt tctatgaaaa ttttgatacc
      661 atgagtaact cagccataag cgcataaagc acaagttact ctttacttta ataaaacttg
      721 acaagaaaag aatttatcct tattctactc taatagagtt aatttgtcaa taaagaataa
      781 aattattttc aaaatatttt cattcttcat tcttatctgt tattatttta taaaatatca
      841 cttaattaga ggtaatttta gtattgtttt tagtccctga ctatcaatgc tcgtaatatc
      901 taatttacct ctatgtgcat taataatgcc agatactatc aaaataccta gaccatgatt
      961 ttgacctttg gcatatgcct cacttagtcc attttggctc tttaataaac tattaattct
     1021 ttcttgactc ataccttttc catcatcttc aacaattata tagcaaaaat ctaaatcaga
     1081 cttagaggta acccttatat tacatccttg ttggttatga gttatactat tttgtattaa
     1141 attactaata gcccttttta ataatttttt atctgcatct ataataatat cttcatttag
     1201 tatatcaagt tcaacgtaaa agtcattatc caaattatta tttataaact cagaaataag
     1261 ctctctcaaa aacgcagaaa ctctaatctt atcacaattc aatgtttgca tattatattc
     1321 aagtttagac actaaattta ggtcatttac taattctctg attttaatcc cttgattgca
     1381 tataatagac acttgctctt gttctctatt tgaaaggcta ctgctttctt caagttctcc
     1441 agcatatcca attatcattg aaagtggagt tcttatatca tgagatattc ctgcaatcca
     1501 gttagaccta gcttcctctt tcttcctaag tttttcatct ttcatttgca actctttaga
     1561 tacagtatta atagattttg aaatatcact tagtattcct ttttcttgta aaagtactgg
     1621 ttctcctttt gacataagct ttatgccagt aataattggt tttatagatt tcaccatttt
     1681 taaacctatg aatagagcta ttaaaaaagt aactattgca ttaaccagaa acatacctat
     1741 tattaacata ggaaattgtt ctatttcctt aaccgtataa gtaaggttaa acttagtata
     1801 cttgtctttt ggataacctg ttactaatat tccatttgga tgttcccata catacacagg
     1861 atagtccttt aaaaagaacc ttgaaaactt tgctacatct gtcaaagtat aatgacttgg
     1921 aatttctttt ggtaaatcaa atccccatat tctatctcca ttgttatcaa ttaacattgc
     1981 ccaaacttta tttttatgta gtacttcctt actagaatca tctaaaataa actttccatc
     2041 tcttaaaatc aaattatctt caatcataat caagtttgat tttatttctc tactatcact
     2101 tgtatctaaa ttatcagagt atttagatac aaataacata tatgaaaaca gaagaaaacc
     2161 tgctatattt aatacaataa aaagtaatga aatcaagata cttgaaccta taaatcgcct
     2221 caatatgtac tttacactat ccaaaattaa atcactcctc ttgatttctt ttattctaat
     2281 atctaaaatt aattaatcat caacttatat ccaagacctc ttactgtaat aagatgttta
     2341 ggtgtagatg gagttttttc tatcttctct ctaacccttc taacatgaac cataagggta
     2401 ttttcatatc catacatatc atctccccaa atggcttgac atagtgaatc attagtaaca
     2461 attctccctt tattttcata caactttaaa agcaacgaat gctctttagc tgttaaagaa
     2521 atctcttgcc catctttctc aacacatgca ctatttaaat tcacaacaac agaatctccc
     2581 agtttaaata ctggcaactc ctcttcttct ataggcacat atacacgatt taatataccc
     2641 acaagcctta aagtaagttc ctttggtaaa aatggcttta ctatataatc gtcagcccct
     2701 aagcccaaac caatcaatct atcttcatct tcacctcttg cagagagaaa aatcactggt
     2761 attttagatg tctctctaat ttttctcata atagaaaaac catctccatc tggcaataca
     2821 acatctaaaa ttgcacaatc tggctttaca tttctaaata ccctaacagc ttcctcataa
     2881 cttgatgcta aaaatactct actaaatccc tcttttctca ggatttcatc tatcattttt
     2941 aataattcct tgtggtcatc aaccacaagt atttttttat cttttattga ctccataatc
     3001 atcaccctcc tttagtatac catataccca ttttcacaac tttgcctaaa tagactttta
     3061 aggttattgt aaggtaaatt tcagctaatt gaaatgtaac caagttatta tactgatata
     3121 agttaaaaga atcaataagc acagattaaa aaaggggatg aaacatatgg aaatgataat
     3181 tagaacacaa agactttcaa aggaatacaa taaaatattt agagtaaagg atatagacct
     3241 aagagttcca caaggagctg tatatggttt tttgggtcct aatggagctg gaaaatctac
     3301 cactcttaaa atgcttcttg gacttgctaa acccacacaa ggaaatatta atattttagg
     3361 taaagagtta aatgaaaaaa atagaatatc aattttaaaa gatataggtt ctttgataga
     3421 atctccatca tactatggtc atcttacagg gcttgaaaac atgaccataa tgcaaagact
     3481 cttagattta cctaaaaaaa atgtaaatga agcattaaaa attgtaaggt tagaaaatca
     3541 aaaaaataaa aaagttagtc aatattcttt aggtatgaag caacgtcttg gcattgctat
     3601 ggcaataatg aagtttccca aattattaat acttgatgaa cctacaaatg gactcgaccc
     3661 agcagggatt gaggaaatta gagaattaat aaaatctcta ccagaaaaat acgggatgac
     3721 tgttctaatc tctagccatc ttttaagtga aattgaccaa attgccacat caataggaat
     3781 aataaatcat ggcgaactgg tttttcaaga tagcataaaa gaacttcata atagaggtca
     3841 aagtcagatt gctatcaaaa ctaagaatgt tgtatctgca caaaaactat taaatactaa
     3901 aggatatatg tcacttatac aagatgggta cttgactatt gaaaatttaa aagactcaga
     3961 tgtcgctaaa ataaatggta tgcttgtcca ttcaaatata gatgtattta gaatagaaga
     4021 acataaaaaa agcttggaag atatattctt agaacttact ggtaaggcgg tgactttata
     4081 atgaaatacc ttggtctaga attttataaa cttaaaagaa agaagattat tctgatgacc
     4141 tttttatttg cagctgttga aataatgtgg tgtatcgtaa attcaaatag agctattaca
     4201 cgtaatccag atatgttagg tggatttgaa tattcctata tgcttatgag ttttgcaagt
     4261 ttaaatggtc tattctttcc tatcttaata tcaataatta catcacgtat aagcgatata
     4321 gaacataaag gagatacatg gaagctttta aaatcttctg tgacatcttt aaatagtatt
     4381 tatttatcta aatttttatg ttctgctatt ttggtatcta tacctgtatt gatgcaagtg
     4441 ttatctattg tgttgtttgg acattttaga ggggttattg agtcaatttc tgtatcttta
     4501 cttttaaaat acacattagg aacaattctt gtaagtattg ctatcattgc actacaccta
     4561 tggattgcaa ctgtaattcc aaatcaaatg attgcaatta catttggcat gattggtagt
     4621 tttataggtt tgacatcagg tttgttttat caaggtattc gtagattgtt tatatggtct
     4681 tattacctag agttatcacc tttaagttat gcttatgata atgttttagg ttcatctgtc
     4741 tatagaattg atatgaattt ttctattacg attttagttt tcttaattgg aatgctaatt
     4801 tattatattg gaagaaatca tttattaaaa aaagaagtat aggagggatt taaatgttaa
     4861 ggaagtcagt tttgggagaa attctaaagt taaaaaattc ttttatatgg tatgtattat
     4921 tagctttgcc acttataagt gtgttgattg gttctggtaa tttttatcta aatcaaggca
     4981 tactcaaaaa agaatggtac agcttatgga ctcaagttag tcttttttat ggggaatttt
     5041 tctttcctat cttaatcgca atcttttgtg catatatttg taggcttgaa catatgaatc
     5101 ataattggaa taatgtaatg acactgccag ttaatttaaa aaatatcttt ttatctaaac
     5161 ttacagtagt ttcaatactt acagctatta cacaagtttt ctttgttatc ttttatatag
     5221 ttgcaggaaa tatgtttggt ttttctggta gtgtacctaa agaattgact ggttggattg
     5281 ctaaaggttg gattatttct atttcaatag cttctataca attatacatg tctataaaga
     5341 ttagaagttt tgcaactcct attggtatta gtctttgcct ttctctattt ggtatgggat
     5401 taatagtagc aaaacctgct gttggtatat ttttccctta ttctatgtta ggtagtgcta
     5461 tgggggttat aaagcaatct gctttaaatg ttaatgacac tatagttaca atatgtgttg
     5521 catttattac tactatagta ttttctgaaa ttgccaatag aaattttaga aagaatgatg
     5581 ttatttctta atggtaaatg ctagatattt ttgggtttga aatttatatt ctttttagtt
     5641 gaaatctata tccattttat ttaaaaatat atatctttta tttgaatttt atatgtttta
     5701 tctactcgct atataataga tatactccta aaatttaaaa atatataata atgttattat
     5761 ttaaaattat tatgtattat attaaaactt aatgcaaaaa caatttttat attatataat
     5821 gaaacttaat taaatatagg taaaaaatca ttcaatatat taaataaata tttaacttta
     5881 ataatctata tttaattata gctataacat agaattatca aatatattga atgaatattt
     5941 gactcttgga atgtgtaatt aaattaagtt tggttattcg atggaaggca aatttgatat
     6001 aaaactccat ttaaagtgcc agataaaaac tttatggtta tataaaagtt aaaagtaatt
     6061 tatagtgtta tagaacattc aaagggattt aaagtaatct atggtgtttt gaaatattca
     6121 aagggattta aactaatcta tagtcttgta gaatattcaa agagatttaa aacaatctat
     6181 aatgttgtaa aatatattca aagggattta aactaatcta tagtcttgta gaatattcaa
     6241 agagatttaa agcaatctat aatgctgtaa aatatattca aggggatttg aagtaattta
     6301 ttgtgttttg aaatattcaa aaagattttt ggaatagttt ttataaattt accagtacta
     6361 ataattttat gtttaacatt tagatgacct aaaatcaact atttgcactt ttgtattcat
     6421 ctaaaatcat tttataatat ttttaaataa tggaaaggat gtttgaatat gtttgaatca
     6481 agatgtggtg tctgttgtga cagttgtaca aaaaaagaac aagtaaattg cactggttgc
     6541 ccaactatgg aaaaaccatt ttggggtgga gaatgtaagg ttaaaacttg ctgtgaaagt
     6601 aaggaactta atcattgtgg tgaatgtgat actttccctt gtgatatgct tttaaataag
     6661 ggaaaagacc aaggttttga ccctatggta aatattgagc aatgtagaaa atggcttgaa
     6721 gaaacagttt ctaattaatt gttttagaat gctttgttaa ggaatttatt taatcctttg
     6781 ttaggtagta tgtttagtca tttgttgatt agtatattta tacatttatt gatgggtata
     6841 tttagccata caaatagatt tacataaaat ttacgactaa aaaagagttg agtattagtt
     6901 tttattattt aaaacggata ttcaactctt ttttatgtct aacttttttg attttttaat
     6961 ctttccaata ccaccacttt aatttttctg gttctggatt ttcagttgat gcaaaatata
     7021 ctgcacatct gtatacatat aactgacatc tgtctatgtg ctgtccacgc attaaacatt
     7081 ccttttcata catttgtgct gggtttgcat ctttaagtga tttaatagtt gtatatccta
     7141 acatgattaa agatttttct gttgctttcc ctacccctgg aattttttgt agttctccca
     7201 tacttcacct tctttctggt atattctttt tatattatac ctattaccag atattttttt
     7261 attatagtta atatttaata tttaattttt tattatatca atttatttat gctctttcat
     7321 atatatatat tttaccacct ctaaatcact gagtcactta attacatcat aatatagttt
     7381 tatacaaata aaatacttta tgtttaattt aatatataaa attcaccttc aagaaaatta
     7441 tattataatc tgacattttt atcttatttt ttcaaaatat attgaatctt cttactttat
     7501 tttgtaaaat tatgcttagg ggaaatatat tttaggaaaa tatgaatata taatttttag
     7561 tcaactagtt attttaagtt tttaactttt aaaataaaat atatctaata aaagggagat
     7621 tgtattatgt tttctaaaaa aaatgagggt aacgaattta gtaatgaagg aaaaggaagc
     7681 tctaagaaaa taattaaatt ctttaagagc acaaagggta ttgctctact ggcatttatt
     7741 ttaggcgtgt tttttggcaa tatatcctca ccagcttgtt ctgaagacca tgaggaggtc
     7801 atttctaacc aaacatcagt tatagattct caaaaaacag aaatagaaac tttaaatagc
     7861 aaattgtctg atgctgaacc atggttcaaa atgaaagacg acgaaaagaa agctattgaa
     7921 gctgaaaatc aacgtaaagc tgaagaagct aaaaaagctg aagaagctaa aaaggctgaa
     7981 gaacaacgca aaaaagaaga agaggagaag aaaggatatg atactggtat tacttatgac
     8041 caattagcta gaacacctga tgattataag tacaaaaagg taaaatttga aggtaaggtt
     8101 attcaagtta ttgaagatgg tgatgaggtg caaataagat tagctgtgtc tggaaattat
     8161 gataaggttg tactatgtag ttataaaaaa tcaataactc cttcaagagt attagaggat
     8221 gattacataa ctataagagg tataagtgct ggaactataa cttatgaatc aactatgggt
     8281 ggaaatataa ctataccagg gatagctgta gagaaaatta attaatattg ttttgtatta
     8341 tagttaatat tttatattat agtcaatatg tttaaagatg tttttataat tgcaaataaa
     8401 cagttacaag gctctaaatt agtttttgct
//
LOCUS       DAYATN010000151         8393 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.152, whole genome shotgun sequence.
ACCESSION   DAYATN010000151 DAYATN010000000
VERSION     DAYATN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.152"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..415
                     /locus_tag="ACGI8A_003220"
     CDS             <1..415
                     /locus_tag="ACGI8A_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0181199.1"
                     /translation="IKKYSKYFISKKIILDLNNLNFKVLSDEKWLYFIFEQLLSNSLK
                     YSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDLNRIFNKGFTGNNGRNNSKSTGLG
                     LYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL"
     gene            527..1756
                     /locus_tag="ACGI8A_003221"
     CDS             527..1756
                     /locus_tag="ACGI8A_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFH0181200.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALSSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            1764..2438
                     /locus_tag="ACGI8A_003222"
     CDS             1764..2438
                     /locus_tag="ACGI8A_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181201.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            2441..3673
                     /locus_tag="ACGI8A_003223"
     CDS             2441..3673
                     /locus_tag="ACGI8A_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181202.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(3959..5224)
                     /locus_tag="ACGI8A_003224"
     CDS             complement(3959..5224)
                     /locus_tag="ACGI8A_003224"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFH0181203.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(5277..6425)
                     /locus_tag="ACGI8A_003225"
     CDS             complement(5277..6425)
                     /locus_tag="ACGI8A_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFH0181204.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(6516..7061)
                     /locus_tag="ACGI8A_003226"
     CDS             complement(6516..7061)
                     /locus_tag="ACGI8A_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HFH0181205.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(7284..8228)
                     /locus_tag="ACGI8A_003227"
     CDS             complement(7284..8228)
                     /locus_tag="ACGI8A_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFH0181206.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         2916 a         1387 c         1104 g         2986 t
ORIGIN      
        1 tataaaaaaa tactcaaaat attttatttc aaaaaaaatt attttagact taaataattt
       61 gaattttaag gttttatctg atgaaaaatg gctatacttc atattcgagc aattattgag
      121 taattcccta aaatactcct cattaggaga taatatatct atctattgca aaagaaccaa
      181 aaattattta caattaagag tggaagatac tggttgtggt ataaaaaagg aagatttaaa
      241 tcgaatattc aataagggat ttacaggtaa taatggaaga aacaattcta aatctacagg
      301 tcttgggctt tatttagtaa aagaactttg tgataagctt ggtcataaaa ttgatgtaga
      361 ttcagagtac aaccaatata caaaatttac tattacattc aaatgtgatt tatagtatct
      421 tattattgat agtttatttt cattaactta tattcaactt cttatagtaa ttctatatgt
      481 acatcccagt atataattaa gtagttttta taaaaaggag gaagacatgt cttttctaaa
      541 aaaatttaaa aataaaagat ttagaaataa cagttttaaa agtaacaact ttagaaatag
      601 cagttttgga agtagtggct tcaagtctaa aaataaaggt tttaaaaaca atccctttaa
      661 aaataagaga ttcaaaatat atgcaggaat agcagtaatc atacttttaa ttctaggagt
      721 tcttgcttat gttgattcga aaaacactga attacaagct aatgaaaatt ttatcgaaac
      781 atatactatt cctgaaaatg aaaaaatatt tataaatggg atggttgttc ctaagcaaac
      841 taaggacttc aatatatctg gagattatga gttaagtgat gttaatgtta caaatggtca
      901 aaaagtaaat caaggagacc ttctttttac tgctaaaaat ccaactataa tagcagaaat
      961 agatagttta aaatcacaac ttagtcaata caaaaaacaa aaaatatcac tttctgatat
     1021 tgctgaaaat aaagatgcta ttgcttctat aaatgcacag ataactgctt taagctccca
     1081 gatagcttct ctggataaaa aagcttatga cagagttact gctccttttg atggcacagt
     1141 atacttgaat gaccaaactg gaaatccaga ccaaccagtt tcatttatga ctattcaagg
     1201 tctagagttt tatatgaaag gacaagcaag tgaacaagat ttacctaaaa tgaagattga
     1261 ccaaatggtc aatatactag ttttttcaac tgaccaaaag cttactggta gaataagttt
     1321 tatatctgat aaaccatcta ctccaaatac tgaaatggga gctcagcaga atacactatc
     1381 ttattatgat ataaacatag catttgataa tcaagaagga ttagtaaatg gattccatct
     1441 tcaagcatca cttgaagtag ctaattcaag ctttaaaata ccagcatctt gtgtacttaa
     1501 agacaacaaa cactcatatg tatttaagga tttagatggt atattgaaaa agcaaattgt
     1561 agatgtagca agtcaaaatg atgattttgc tgtagttaga ggcggtcttg aacaaggcga
     1621 tataataata aaacacccta ctaaagaaat gaaagaagga gacccagttc aaggcgatgg
     1681 cgtaactgct ggttctaata atggagatac tactccaaac aaaaaagtag aagaagttac
     1741 tatggatgtg aattaattta catatgttaa ttaaattaga aaatattcaa aaatattaca
     1801 aagtagggaa agatgaatta catgttttaa aatccttaaa tttagaaatt gaatctggag
     1861 aatttgtcat gataatgggt aagtctggta gtggtaaaac tactttactt aatatcttag
     1921 gttttctaga tgtatttgat gaaggaagat atatatttga tggaacagat gtaactaatt
     1981 tgagtgaaaa tgaacgttct gtttttagaa atattaatat tggatttgtt ttccaacaat
     2041 ttaatttaat agaaactcta aatgtatatc aaaatgtaga gttgcctcta atttacaata
     2101 aagcactaaa aaaatctaat agagaagaaa ttgttaaaga taaattaagt tctgttgggc
     2161 ttttggacaa gcttaagcag aaaccacttc aattatctgg tggtcaacaa cagcgtgtag
     2221 ccattgcacg ttgcctagca aatgaccctc agataatctt tgctgacgaa ccgactgggg
     2281 cattagatag tgaaactagc agagaaatta tggagctttt aacaaggctc aacaaacaag
     2341 gtaagactat aataatggtt acacatgatc aagatttaac taagtatgca actaaggtta
     2401 tacgacttaa agatggtgtg tttacttcgg aggtgtaatc atgagtttat taaaaaattc
     2461 tttggccaat ttaaaaggtc acaagcttcg tgtatttgta gctttactct ggattataat
     2521 aggaataact tcagtaatac ttgttagttc tataggtaat ggatttcaaa aagaaattaa
     2581 gaaatctgta aacaatgtaa accctaataa aactactatt tcgtttgaat cagccgataa
     2641 cacaggtctt actgatgata tgagtatttt tttaaaacca tttaatgcaa aagatttaga
     2701 agaattatca tttgttgaag gagtagaaag aatagctcct tcaagagatg ggtttaacct
     2761 tgattcagtt tactcttcac aagcatcttt tgataaaaaa actacgtatg ttgatgttgg
     2821 accagtaaaa aaagattcta aaataaattt aatttgtggt agagattttt ctttagatga
     2881 tgaaaaaaga aaagtaattt tacttacatt acaaagtaca agtgaaattt ttgaaaatcc
     2941 agaagatgcc ttaggtcatg gtatcaacat aaatggaact atatttgaaa taataggggt
     3001 attagatgat tctcagcaaa accaagctgg aggattcttt ggaggttacc aagatatgca
     3061 atttactact tccctagtac ctaaaaaggc ttttgacact ttaatgagtc aaaattctta
     3121 ttcaaatgaa atttaccagt tagacttagt ttcttctaaa ggctataatg taaatgaagt
     3181 tgcaaacaat gttatagcta aattatatga gatgcaccct ggtatcaatg gttcttatac
     3241 tacaccagac ccaactgaac agacagctta tcttgaaagc attaattcca atgtaaacaa
     3301 atatgtatct ataataactg tagttgcaat gtttgttggt ggtattggtg taatgaatat
     3361 aatgtatgta tctgttatgg aaagacaaag agaaattggt ataagacgtg ctattggagc
     3421 aaaacctagg tctatattat ttcaattttt agttgaagct gtttttataa cagtatgtgg
     3481 tggaatttta ggaactatag ttggatttac tgctactaac tatgtttcta aatatatagg
     3541 ttttgaagct atacctagct taaatagctt attttatgca attgtagcta ctatattgac
     3601 tggagttgta tttggtctga ttccagcctt taaagcctct aaactagacc ctataaaggc
     3661 aatttataag tagaaattgt ataaagattt tcgattttta gattagttta tataaaacat
     3721 acttaaatat ataacgcact attatatatg atagattagg aacttaatct ttccaaatat
     3781 aatagtgcgt tctttcataa aaaggattct cttccttact atatctaaat taccaagatg
     3841 ttatgttcta attgttcaaa ctaatacttc caattttgat taatcaatct cattctccta
     3901 atatacaatc ttttactttc aatcattgta cactccgcct aaaaaaaata atcttaaatt
     3961 aggcgttata tacaatctcc ccattacata ttgtataatt aacctttcca tataaagatt
     4021 tacctttaaa tggtgaatta tcagctttag aaacaaagct ttcaactgtc cattcttctt
     4081 ctggattgaa tataacaatg tctgctacag ccccttcttc tataaatcca atatttaaat
     4141 tgtacaactt tgcaggattt atagacattt tttctattaa ctgcatcata ctaagatgat
     4201 ttttatgcac caaatttgtt attccaagag ctaaagaagt ttcaagccca ataattccac
     4261 ttggagcttt tataaactct ctatcttttt cttctttact atgaggtgca tggtccgttg
     4321 ctattatttc aatagtacca tcatttaatc cctctataat cttatctctg tcccatttac
     4381 ttcttagagg tggattcatt tttgcatttg ttccaaactc caatacatct tcctcagtca
     4441 atgtaaagtg atgtggtgaa gcttctgcaa ctacatttgc acctaagtat tttgcaaatc
     4501 taaccatatc aacagataca cctgaactta tatgttgtat atcaacttta gcaccagtct
     4561 ttagtgccaa catacaatct ctggctacca taacatcttc tgctacactt gatgcacctt
     4621 ttaatccaag ttctgaagct acctttcctg catttacacc tggatttcca actaaagaag
     4681 ggtcttcttc atgaaaacta agtggtacat ccaactcttt tgccttaatc attgcctcca
     4741 taattagatt gctatccatt aaaggtaatc catcatcagt aaaccctggt acaccagctt
     4801 ttttaagagc ttccatatca actaactctt taccttcaaa acctttagtt atagctgctg
     4861 cttgtaaaac atttatacat gcattttttg acttttcttt aacataatta aaagtatctt
     4921 cattatctac aataggatta gtatttgcca tacatataac agttgtaaat ccacctctag
     4981 cagcagattt agctccactt tctatatctt ctttatatgt aaacccaggg tctctaaagt
     5041 gtacatgtac atccactaaa cctggagcaa ctacattacc actagcatct attatttttt
     5101 catattcatc actttcatca aacttgccta ttttataaat tttattttct tttataatta
     5161 agtcaatatt ctcatctctt tgacttttag ggtctattac tctaccattt ttaattaaaa
     5221 tcatattatt tctcctttat atttttaatt tatatagact catataatat tatacattat
     5281 tttacatact taaatacaaa attacgtttg acatcaatag cctttttagg acaaagttca
     5341 tggcaacagt aacatcttat acatttactt aagtctataa tagcaccctt agtccctatc
     5401 tctatagtgg atgcaggaca agcttccttg cattttccac attttatgca atcttgaaat
     5461 cttaccacag gctttggtgt tataagcttt gaaataggtt catttaaaaa ttttggtagc
     5521 tttacagaaa acaatcttaa atccttactt acatttggaa tcttgtaatt ttttataatt
     5581 aaatctttta tatcttcacc taaaatttca atatctgaaa aatcttcttg tatatatcct
     5641 ctcttaatag cacctctaat tgtagcaact ctatttggaa gtaaacttat aatcttacat
     5701 gcaacaacat ctatagcata tggattttgt gatgctaata aaacgcctat ttctcgagta
     5761 gtaccagctg aaggtccatc accttccata cctactatac catccataat tgtcaatgtt
     5821 ggttttatat aatcacaaat atctaaaagt acattttcgc aaaacaactc ttctgtagga
     5881 aatctataat gtatctctgc ttttttcaat ccagctatgc atccatataa gttttttgta
     5941 ccaccagtaa aagttgccat tatatgagtc tttaatttac aaagatttat tatataatct
     6001 gaatctacta ttggtttaat aatatccata tattttagaa catgtgcttt ttcactatgt
     6061 atcttaacat ctgaaacatc ataatttaac ttaatgccca gttcatttgc tacttcttca
     6121 attccagatg ccttataaat gctcttaaga gaactttgat tatatggacc accagggcta
     6181 tctgctattg taatatcaca atttaactct aaaagttttt cacatacaac ttttacaacc
     6241 ataggatgtg tagttgtagc ttcttcaggc ttttttttca ttaataaatt tggttttata
     6301 agtactttgc ttcctttttt aacaaactta tctataccac caatatcctc taaatttttt
     6361 tcaatagata actttacttc ttcataattg taagttttac aactcctaac agataccgac
     6421 ttcatattat ctccatcctt ctatcacaac agtttatttg tataatttaa aaaaggagaa
     6481 accaaagttt ctccttttaa tctatgtttt aattattatt ttccaaaatg tctgtttaat
     6541 aatcctaaga atgctttacc atgtctagct tcgtctttac acatttcatg tactgtatca
     6601 tgtatagcat ctaatcctaa ttctttagct ctcttagcta attttaattt tccatcagtt
     6661 gcaccatact cagcatcaac tctaactctt aagttttctt ttgtatctgc aactacaact
     6721 tctccaagaa gttctgcaaa tttagcagca tgttcagcct cttcaaaagc tattctctta
     6781 tacgcttcag ctacttctgg ataaccttct ctatcagctt gtctactcat tgctaaatac
     6841 attcctactt ctgtacactc accagtaaag ttggctctta atccttcgat tatttctgca
     6901 tctacacctt gagctactcc tattctatgt tcatcagccc aaaccatttc gcctttcatt
     6961 tcttcaaatt tatcagctcc aactttacat actggacatt gtgctggtgc agcatctcct
     7021 tcatgtatat atccacatac tgtacaaaca aattttttca taattaattt cctcccaatt
     7081 ttatataatt aaatttttat ttttaagttt aataacttgc tacaagaata tatatacccc
     7141 agactttata gcttaaacac taaaaataaa atttatttcc taattaattt tatcactttg
     7201 aaacaccagt atcaaccaag ttttgatgtc aaatttctac ataatacata atttatttga
     7261 ttaattagga aatagttgtc atattaaata ctttctaatg aagactcctc tgaatcttta
     7321 agtttcactt ttttgctctt taaaaaagat aatttagttt ctgcagttat gattgcaaca
     7381 aatatcaaga tactaccaat aaccaatttt gctgtaataa gctcctttaa aattattata
     7441 gaaaatattg taccaaatac agcctctgtg gataatatta tagctgttct agttccatca
     7501 actctttttt gacatatagt ctgaaataaa aatccaattg tagtactaaa tacagctaga
     7561 tacatagttc ccatgtaacc agataattca gcttccatct ttaattgacc tgcaaatgtt
     7621 tgaactacaa cagacattaa aaacgctact gtaaattgaa tagctgtcaa tgccattggg
     7681 ttgttatctt ttgcaaactc acttgtaaag aatatatgaa atgcaaatcc gaatgaacat
     7741 ataagtgtta gaaaatctcc aaaattaata gagaaatcag cttctaaaga aagtatccca
     7801 attcctataa gagctaccaa actacttatt attcctattt tatcaagttt tcttctataa
     7861 agtataaatc ctatgaatgg aactattaca acattagctg ctgttataaa cgcattttta
     7921 gatggtgtag tgtacataag tccaatagtt tgtactgtga atgctagaaa taaaaatatt
     7981 cctaaaatcc ctcctgcttt taataatttt ttacccatat tagcacgaat ctgtttaaaa
     8041 aacagtaaat tcataattat agctgccaag aaaaatctta aagttatgat ttgtagaggt
     8101 gtaagcccac cgtctaatgc aaattgggtt gctacaaagc cacttcccca tataattgct
     8161 ataaatatta gtcctatttc tccaaagtat tttttcaata aagccccctc ctttttattt
     8221 ttttgcaaaa ttataaaccc cctaaacagc aagagagttt ttctataagc attactataa
     8281 attaatatag taaatttttt cagtatttta aatctaaatt tacatacatt ttgagctcaa
     8341 atttatatcc actatttaac agtaatatat taatcatttt ttgtaaacac ctt
//
LOCUS       DAYATN010000152         8307 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.153, whole genome shotgun sequence.
ACCESSION   DAYATN010000152 DAYATN010000000
VERSION     DAYATN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.153"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..572
                     /locus_tag="ACGI8A_003228"
     CDS             <1..572
                     /locus_tag="ACGI8A_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HFH0181207.1"
                     /translation="KKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELRENT
                     INNSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFMD
                     YRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEAFFKENNLVEINYKVQTYTYEIFSV
                     YTADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(1585..2247)
                     /locus_tag="ACGI8A_003229"
     CDS             complement(1585..2247)
                     /locus_tag="ACGI8A_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181208.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(2264..4819)
                     /locus_tag="ACGI8A_003230"
     CDS             complement(2264..4819)
                     /locus_tag="ACGI8A_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0181209.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNIRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGMISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLISVVVSNRDFESMNIIEGIKENE"
     gene            complement(5059..5247)
                     /locus_tag="ACGI8A_003231"
     CDS             complement(5059..5247)
                     /locus_tag="ACGI8A_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HFH0181210.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTK"
     gene            complement(5259..5438)
                     /locus_tag="ACGI8A_003232"
     CDS             complement(5259..5438)
                     /locus_tag="ACGI8A_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181211.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEVNVEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(5443..5700)
                     /locus_tag="ACGI8A_003233"
     CDS             complement(5443..5700)
                     /locus_tag="ACGI8A_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181212.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKEKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(5673..5813)
                     /locus_tag="ACGI8A_003234"
     CDS             complement(5673..5813)
                     /locus_tag="ACGI8A_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181213.1"
                     /translation="MVIAISLTLGLITGCTNKVSGTNEKIYTIEQISSIKKTCQRKYQ
                     NQ"
     gene            complement(6080..6286)
                     /locus_tag="ACGI8A_003235"
     CDS             complement(6080..6286)
                     /locus_tag="ACGI8A_003235"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFH0181214.1"
                     /translation="MCKYLDNAIEACDKIHDKSIDKYIQIKGTISKSYFVIKCENSKI
                     NSIRKNKSLLLTDKKDKLVHGIGI"
     gene            complement(6462..6707)
                     /locus_tag="ACGI8A_003236"
     CDS             complement(6462..6707)
                     /locus_tag="ACGI8A_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFH0181215.1"
                     /translation="MIPRLILASSLVIIIVFIKIIKENKLKLQNEVIKNKLDMQYSHY
                     LSIQESHAKVKKLYHDINNHIYCIENLKYKKGKLMGM"
     gene            complement(7347..7670)
                     /locus_tag="ACGI8A_003237"
     CDS             complement(7347..7670)
                     /locus_tag="ACGI8A_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFH0181216.1"
                     /translation="MKKIKINLTHTKSNLAKKYIEVQKKNMLIHTKNKTLDAICILEK
                     IEKDLNIEELVRCHKSYITNLNYVENIKSNTAILDSQEEVPISRYRYKDIKEGFLKFI
                     GDRIC"
     gene            complement(7909..8061)
                     /locus_tag="ACGI8A_003238"
     CDS             complement(7909..8061)
                     /locus_tag="ACGI8A_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181217.1"
                     /translation="MKNLNKNTILGEVIITITACDDEKEIQIVIKNQVDNILKNKNKE
                     YRIKKI"
BASE COUNT         2781 a         1201 c          859 g         3466 t
ORIGIN      
        1 tgaaaaaact agtaaaaata ttaataaata ttatttgcat tattacgcta attttctcta
       61 gcctttcgat ttacataaaa ttatctgaat ataaaaaagc tgatgaagta tatacggaac
      121 tcagagaaaa cactatcaat aatagcaaac atcaagaact ttatgataaa aataatgatt
      181 atagattctg gcttaaaata aacaacgtaa atatagacta tcctgtagta caaggatata
      241 acaatgattt ctacctaact catgattttt ataaaaatta tcttccttta ggtagtattt
      301 tcatggatta tagaaataat tttgaaaatg ataagagttt aatagtctat ggtcattata
      361 tgaaaaataa aactatgttt ggtcagctag aaaactatac tgatgaagca tttttcaaag
      421 aaaataactt agtagaaata aattataaag tacaaactta tacttatgaa attttttcag
      481 tatatactgc tgacttaatt aacagagatt atttaagtat acactttaat agtaattatg
      541 agtttaaaga ttatttgaat tatataacct aaaaatcagt acacaaatct gatataaagg
      601 tagatatttc agataaaatt ataactttgt atacttgtaa ctatgaattt aaagatgctc
      661 gtactatagt tcatgctaaa ttaatttcaa aaaaagagac tgataagtag gtttgtgtaa
      721 atgcagactt acctacagtc ttttttgaac gtaaaattag taagtatgga ataatattca
      781 tattaaactt ttgtaggcaa agaaatgaaa aatcaaagag aaataattat tacagattta
      841 gattaccaag ctgaattaag aaagtgtaaa actatggagg atgtagttgg tgaagatagt
      901 atataatagt ttaacttata aattatcttg gtgatattaa tgaagtttca aagtatatat
      961 tatttattga tataaataat attaaatctg ttgctaacag atgtaccttt gtttggagac
     1021 aaactattta agaaatataa ctaaaatgtt gaagtagcta cctgacttta ttttgaaatg
     1081 cgaacaagat tttggcatta agataattta gaaagaatca ctggtacaca aggatgtgaa
     1141 cttagaatga atagaagtat tcaggcataa ggagctttca cccaaataaa acatagtatg
     1201 aactttaaaa gaattctaat cagaggcaat caaaatgttc tttcatagtt tatacctgta
     1261 gtgttagaca tagtatttat aagtttcaca aataagataa tcattaataa atgtagtatg
     1321 tatattcatc ctctcaaaaa tattttttaa tcttacactt tgtaaattat tctattttaa
     1381 aatggtattt ttataccttt atttcttatg accgttttca agctcaaatt ttgcataaat
     1441 ataaattaat gaaaagcaag taaaaaaaga actgtcgcac agcaatttaa aattactcat
     1501 tcgacagtcc ttatttcgtg gatttaaata gtggagaaga atcacaagaa taatacccac
     1561 gattaaacct ttatacaata cattttatat gactttacca tcactaatag ttattataca
     1621 atcagccatc ttagctattt catcattatg agttatcata accaacgtct gcttgtattc
     1681 atttacacaa tactttagta aattcatcac ctcattagtt gttttactgt ctaaatttcc
     1741 agtaggttca tctgcaaaaa ttattgatgg cttattggca agtgatcttg ctattgcaac
     1801 tctttgttgt tgaccacctg ataattcatt aggaaattta ttaatctgac tttctaatcc
     1861 taacttagtt attaaatcat taatataatt attatctatc tttcctttag caattaaaat
     1921 aggaagaact atattatcat aaacattgat aacaggtatt agattatagt tttggaatat
     1981 aaacccaaat tctttacttc taagattaga tagcttttca tcacttaaag tatataaatc
     2041 taaattatcc ataaatacat tacctaaagt tggtttatca agccctgcca tacagtgaag
     2101 taatgtgctt tttccagaac cactttgtcc aattattgca ctaaatttat ggggttctat
     2161 ctctaaagta attccatcaa cagcctttat ttcattttct ccttttccat atattttttt
     2221 aatatctata acttttaaca tcttcataca tttacctccc aaattattca ttctctttta
     2281 taccttcaat aatattcata ctttcaaaat ctctattact tacaactact gatattaaac
     2341 atacaaatac agatatagca aagaatagta aagattcctt aattgggaat gtaaaagaca
     2401 taaaattctc tattccatag tttatatacg catcattatt ccaatttgta aatttaacta
     2461 gatttataaa tgatggaatc atagctatta taaagctaaa aactgcatat gctaatgctt
     2521 catatataag catttttttc atatttttaa gactaacacc taatgctcta agcgttgaaa
     2581 tttctttttt tctaattagt agatttgttc ttatagtaca gaaaatatta attgatgcta
     2641 ttattaaaac tagtgcaatt attgctattt gtgatttaat gtaactttta tatatacttt
     2701 ctgacatttc atgataactt aatctactat taaatagaga ccctggtata cttcttgaaa
     2761 ttttctgtat ctcattatta acaatctcat catatggatt ttctaaattt atgcctaatt
     2821 gagtgtattt ttgttctcct gttatgtttt tagagtgaat attacttgtt ataatttcta
     2881 gatctggcat aaaatcgcca tctcccatag caatccattc atcctttaaa attacagcca
     2941 ctcttacctt catctcttta taaacaattg tattattttc ttcaacagga atttttacag
     3001 ttattatgtc tcctatattt aattctttga atataggctt tatagtatga tcatcaacta
     3061 tatcataaaa ataattatat actgctacat ttggatatcc atcagtgtcc tttttaatat
     3121 catctaagct accttcttca acaaagtcat ttaacttttc atctatataa tcatcatcat
     3181 aaagctttaa aactaattta tcttccatta tctctttttt ttctactcca ttataggttg
     3241 taaattcttt gtttatatcc ttattattaa aagtaaacat tccatttgtt atttgaattt
     3301 tatgtatact tttaacaccc ttaatttttt ctatattctc tacatcacta tctgtgtatt
     3361 ttgatatttg cgggtctgca tttactccaa attgcattgt tatattatat ctattttgca
     3421 tatcttcaat tttactgttg acctcttctt gcatacttga gaatgtagta atgaatatat
     3481 atcctcctaa agcaatagac aacatagata tagcagctct aaccttattt cttccgatat
     3541 ttttaagtcc cataaatcca taaaatccaa atattttgct tactagtctg tctattccaa
     3601 gtctacttct gtgattttta attttgtctg tatttattat tgattcaata ggtgatatct
     3661 tgccgatttt aaaagttggt ataatacaag atataaaaac agaaatcatt ccaaccaata
     3721 tagcttttat aactatttca ttatttatat ataatttagg gttaagacca gcttcactgt
     3781 ataaaacttc gttataatta ctaattccta tatatgaata tgtaacccca gctactaacc
     3841 caagtagtat acctattatt gttataatta atgcttgata gattatcata caccttatgt
     3901 ttcttttctt tgcgcccatc agtctaagca gtcccatttc ttttactgat tgatttagtg
     3961 taatattgaa aatattaaat atgaaaatta tagatactgt agttattaaa acatttcttt
     4021 taaaaacatt cggatcattc ttatcatctt ctacataacg aagttccctt acaagaggta
     4081 catttggtag aaaacttttt tgtcccaact tatatttttt cataagataa tcacattcac
     4141 cttcaatatt tctccatcca cttttaaaac ttaatatact attataacta atcgcatcat
     4201 gaggtataat gttatttgtt tcatcattgc cataagttac tgcttcgtat tgccaaacca
     4261 catcatagta cccttttggt ttttccataa ctcctactat tttaaattct ttatttatag
     4321 aaaataattg attacttccg ttagtatctt tatagttctt ttttattgtt aaatttaatg
     4381 tagatcctac tccttcactt aaattcattg cttctagtgc ttttttctct aatactattt
     4441 cattgttgtt tttaggtagt ctaccttcta ttattttgtt gtatctactt tttagatact
     4501 cttcgtttga tgaagttaat ataaatgagt ttcctttttt atctgaaata cgtcctagat
     4561 tttgtaccga attacccttt gatacttttt catcagtttc taatttttta gcatcatcaa
     4621 gtgtcaaatc actaaactct acatggtatc cactgtaaag ttcatgtatt gctactacat
     4681 tgttttcatt ttgtgaatct tttataacac taggtccaaa tatcaacata acacctaaca
     4741 taactcctat tattaaaagt aatgttctaa gtttattttt ctttaaatat gctagtgaca
     4801 tttttaagta aatccccata taatcctcct attttactat aaattattta tccaactcat
     4861 ctgttaagta gattggtgga tattttaaat cttttaattc ttcattattt tttgaagctt
     4921 actaccatct tcctcatgat aaacttgcag tatcacctta tttacatgta gtgcataata
     4981 atcaccatta taaaaatatt agtatttata acctggataa tttttgtaaa atgatttata
     5041 aagtatccca tcctaattct attttgtata cttttctttt gtaatacgct ctatatcatc
     5101 acctgaaact ccttgatatg tcatatattt atctatatat ttttcagctt cattccattt
     5161 tctaaaatag ttgttgtata taataaatcc tattatggct ataatgctta taattaaagt
     5221 taataaaata gctttgtatt ttctcatagt gaatcttcct acttcaattt aaattcttta
     5281 ataagtttta attctaaaga cttagtttta tcatcaaatt tcttaacttg aatttttata
     5341 tctcccaaat tatcaacctc tacatttact tcacctttat caaatacttc ttcaattttt
     5401 ctaaaaaatt ctttctcact tatttctata tgaatcaatg atctatatgt tataaatggc
     5461 cattatgaat ttatcatctt ctgacagctt ttcattataa gaaaaatctt taacacattg
     5521 aattgataat atttccttcg tatccttctt ttccttagtt attgaataat gtaaaacttg
     5581 gctaggttgt tcttttgtat actccaagtt tcccaaagac aaaacaaaac ttccatctga
     5641 tttattttca gctaccctaa gaccactatt ttctattgat tctgatattt tctttgacaa
     5701 gtcttcttga tactagaaat ctgttcaata gtatatattt tttcatttgt acctgaaacc
     5761 ttatttgtgc atcctgttat taaacctagt gttaagctta tagctataac catagttatt
     5821 gtaatttttt tatcttcata taatcctcct tatatattga aaactcgtta tttctcataa
     5881 aaaaaagacg ctatcttttt ctattaaaag ataacatccc tatatgttta gtatcaatat
     5941 atgttcccca actggtaggt taatcgaccc aactatcaaa attttgattt aatggtatat
     6001 aaatattcaa tataaatttg tcttttttat cttcaaatga taaatctcct ctatattttt
     6061 ttatagaaga tttgatgctt taaatcccta ttccatgtac aagtttatct tttttatctg
     6121 ttaataataa acttttattc tttctaattg aatttatttt actattctca catttaatta
     6181 cgaaatagga ctttgagata gttcctttta tttgtatata tttatcaata cttttatcat
     6241 gtatcttatc gcaagcttct attgcattat ctaagtattt gcaaatatat tagatacatc
     6301 tattggtttt ataaaattca ctttggaaaa attgatatca caaatgaatt taatttttct
     6361 tttttcactt acatcacttt tttcatttat tattatatcc aatataacat tacctgtatt
     6421 gtaaattgat ttaaattctt taatttaatc ttttaaacta tttacatacc catcaatttt
     6481 ccttttttat atttgagatt ttctatacaa tatatatgat tatttatatc atgataaagc
     6541 ttcttaactt tagcgtgtga ttcttgaata cttagataat gagaatactg catatctaac
     6601 ttatttttta ttacttcatt ttgcaatttt aacttatttt ccttaattat ttttataaac
     6661 actataataa tgactaagct actagctagt attaacctag gtatcacaat atacccaact
     6721 atatatattt cctaataccc accaaatcga tacatttttc tatttatata aattattaat
     6781 atagtaaata aatttgttat taaagcaaca gataagtata tatagtttat tttattatct
     6841 ttatctagtc ccaatctttt attaataaat ttaaatatag cacaagctac tatataacta
     6901 ttattataag ataatagtat atatctactt tagcattatc ataactaaac actaatagag
     6961 cttcctctga tgatattaca ttacttgtaa ctacaaaaaa taaatttata gatataacat
     7021 cttttattaa agggtgatat acgtcaaata agaaaaatga aactatatat ttaacaattc
     7081 ctatcttata aaaataatta tagtaagttc ccaagtaata aatatcaaat aatagattat
     7141 atcatataga tttttcttca tattataatc atgggtctta tcaacattaa ataatataat
     7201 gatcttagtt ataagataag ctaataatac agtaaaaaaa gatattttct tagtcttatc
     7261 tgtatcttta tcgtaagtta atatactttt tagtatcatt attggtgtaa acaatgataa
     7321 tattaataat attttattta tatggtttaa catattctat caccaataaa ttttagaaac
     7381 ccctctttta tatctttata tctatatcta ctaataggta cttcttcttg actgtctaat
     7441 attgcagtat ttgactttat attttctaca tagtttaagt tagttatata acttttatga
     7501 catcttacaa gttcctctat atttaaatct ttttctattt tttctaggat acatatagca
     7561 tcaagagttt tattttttgt atgtattagc atgttctttt tttgaacttc tatatatttt
     7621 tttgctagat tagattttgt atgtgttaga tttattttta tttttttcaa tttttttggt
     7681 acatgttaat atgtgttttt ctagttcttg aaaatttatt ggctttaata agtatcaata
     7741 agctcttact tcatatcttt cctgtacata atttattaga gatgttataa atattattat
     7801 ctacttttct tatgtttctt gcgatatcta tactatttag ttctgccatt tttatatcta
     7861 agagtagtgt atcctctcaa ttaactttgt aatgattctt cttttgagct atattttttt
     7921 aattcgatat tctttgtttt tattttttaa tatattatca acttgatttt ttattacgat
     7981 ttgtatttct ttttcatcat cacaagctgt tattgtaatg ataacctctc ctaatattgt
     8041 atttttattt agattcttca ctttaggtta aaacttttta aatgtattta tcaataatat
     8101 ttaaattaaa aatgtataaa aatacctata tccaattgga tataggtatt cgtggatttc
     8161 aatggtggag acgaggggag tctataagtg gttatttgat tgttttgttg ttttttgcaa
     8221 gtgtaggtat atagctattt agttatgatg ttaaactgat tttttcggat attttttatt
     8281 gtttattttt gtagttgggg tattatt
//
LOCUS       DAYATN010000153         8262 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.154, whole genome shotgun sequence.
ACCESSION   DAYATN010000153 DAYATN010000000
VERSION     DAYATN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.154"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1031
                     /locus_tag="ACGI8A_003239"
     CDS             <1..1031
                     /locus_tag="ACGI8A_003239"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0181218.1"
                     /translation="DMLSNGVAIYFCLTNRCNLFCKHCCVESSSNKKDLLSTYDLKNI
                     VDIISVLNTPHIIITGGEPLIREDFFEIIKYIKQKLPDTKLILSTNGTLIDEHNVDFI
                     ITNFEKIDISIDGVDENTCSETRGKGVFNKVISSVNLLKDNGFSNIKLSMVFGQKNSH
                     LNKQFKDLNKKLGTEFVERYFIPTGRGLENKESYLIEDTVLPITIPYILDNELKMCKD
                     KKNKKMGCCACNAGNSQIFINYDGDVYPCPSLIKDDYKMGNIFDNEFVKVIKENKTRD
                     LKSLIKLESIYPYNFEKCKDCDVNIFCWHCPAILDSVKDNELELDRWCSMMKHNLNTA
                     VWGDEKVN"
     gene            1028..2044
                     /locus_tag="ACGI8A_003240"
     CDS             1028..2044
                     /locus_tag="ACGI8A_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0181219.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPDID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            2100..2261
                     /locus_tag="ACGI8A_003241"
     CDS             2100..2261
                     /locus_tag="ACGI8A_003241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181220.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            2469..5015
                     /locus_tag="ACGI8A_003242"
     CDS             2469..5015
                     /locus_tag="ACGI8A_003242"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0181221.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            5057..6301
                     /locus_tag="ACGI8A_003243"
     CDS             5057..6301
                     /locus_tag="ACGI8A_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0181222.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            6349..7338
                     /locus_tag="ACGI8A_003244"
     CDS             6349..7338
                     /locus_tag="ACGI8A_003244"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFH0181223.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            7375..8049
                     /locus_tag="ACGI8A_003245"
     CDS             7375..8049
                     /locus_tag="ACGI8A_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181224.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            8033..>8262
                     /locus_tag="ACGI8A_003246"
     CDS             8033..>8262
                     /locus_tag="ACGI8A_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181225.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSAT"
BASE COUNT         3500 a          691 c         1342 g         2729 t
ORIGIN      
        1 ttgatatgtt aagcaatggt gttgcaattt atttttgttt gacaaataga tgcaatttat
       61 tttgtaaaca ttgttgtgtt gaatcttctt ctaataagaa agatttactt agtacatatg
      121 atttaaaaaa tatcgtagat ataatatcag ttttaaatac tcctcatata attataactg
      181 gtggagaacc attgataaga gaagattttt ttgaaataat taaatatata aaacaaaaat
      241 tacctgatac caaattaata ttatccacaa atggaacttt aatagatgaa cataatgttg
      301 attttattat aactaatttt gaaaaaatag atattagtat tgatggagta gatgaaaata
      361 cttgtagtga aacacgagga aaaggtgtat ttaataaagt aataagctct gttaatctgc
      421 taaaagacaa tggattcagt aatataaagc tgtctatggt atttggacaa aaaaattctc
      481 atttaaataa acaatttaaa gatttaaata aaaagcttgg aacagaattt gttgaaagat
      541 attttattcc aacaggaaga ggcctagaaa ataaagaaag ctatttaatt gaagatacag
      601 ttcttccaat tacaattccg tatattttag ataatgaatt aaaaatgtgt aaagataaaa
      661 aaaataaaaa aatgggatgt tgtgcatgta atgcaggaaa ctctcaaata tttataaatt
      721 atgatggaga tgtatatcct tgtccttcac tgataaagga tgactataaa atgggaaata
      781 tatttgacaa tgaatttgta aaagttataa aagaaaataa aacaagagat ctaaagtctt
      841 tgattaaatt agaaagtatt tatccatata actttgaaaa atgtaaagat tgtgatgtaa
      901 atattttttg ttggcattgc ccagcgatat tagattcagt taaggataat gaattggaat
      961 tggatagatg gtgtagtatg atgaaacaca atttaaatac agctgtatgg ggagatgaaa
     1021 aagttaattg aaatttacta tatttgtaac tgaagactgc aatttgagat gtacatattg
     1081 ttatgaaggt aatgataaaa aaaaagaaaa tatgagttat gaaactgctg ataaaacaat
     1141 agattttatt tgtaataaag aattagaaag taatagtgat tatacaacaa ttgttttaca
     1201 tggaggagaa ccatttataa attttgattt gattaaatat atacataggg atttagtaga
     1261 tagattgagt gacaaaaaga tattatttga tataactaca aatggaacta ttataaatga
     1321 tgaaataata gagtttatag caaagaatgt aaaaaacata tcgataagta ttgatggaaa
     1381 aaagaatata catgatataa atagaataga tgctaacgga gtaggtactt atgaaaaagt
     1441 aatggaaaat ggaaaaagac ttatagataa aggtatatat gttagggcaa gaatgacata
     1501 taactcaaat actgttaagt atctttatga gagtgtatat gatatagcaa aacaagggtt
     1561 taaaaatatc gcaccaattt cagattttat ggataaaaat tgggataatt ctcatattaa
     1621 aatattaaat gaacaaatta gtaaaattat gaatattaaa aaggattatc ctgatataga
     1681 tatatctatt ttagataaac aatttataaa taagaaaaaa ggagattgtt ttggagggat
     1741 aaatagttat gcaatagata ctagaggaga tgtatatcca tgtacagtat gtgtaggaga
     1801 taaagatatt ttactaggaa atatatatga tggattagat gataaaaaaa ttaagaacct
     1861 tataaaaatt tatatatcta agaatgaaga gtgtgatgga tgtactagac ttgattattg
     1921 tatgtcaacc agatgtaaac ttatcaataa aaaggtaaca ggtaaattcc aaagtgcagt
     1981 accaatttat tgtggattag aaagagttta tgtggaaaca acaaaaaaac tatgtagttt
     2041 ataatctagt ttaactagtt ataataaata taaaacaaaa gtaaaggggg atatgtatta
     2101 tgatacatat aaaaaaaatc gaagaaaaaa tagaacttca agatgttgag gtgcaagcag
     2161 atccatgtaa atggtattat gataagcatg gaataatttc acaaatagac tgtatggtgg
     2221 attgtgcagg atcaactcca aaaggaacag tagcttatta aaaaatacta atcagtctat
     2281 taaatatgtt gaaagtacta gatgtagttt tttacaaatg aaatgtgatt ttctcaactc
     2341 tagtattttt aataattaaa tttaactagc acaacgagtt agttagtatt taatgaatag
     2401 tgctctataa cctactattt taggttatag agtttttctt gtaaatatga ataaggagga
     2461 ttctgattat gataaatatt aaacttgcgg tgtcatattt aaagagacaa aaaggaaaaa
     2521 ctttatcatt aatacttagt atagggattg ctgttatgct agttttttca ttaagtgtta
     2581 ttaatgaatc tcaaggacaa tcagatataa atcatgcata caaaaactcg ggagattatc
     2641 atgcttttta tgaagatata aatagtgata tagtaaaaga acttaatggt gaagaagata
     2701 taaaagaatt caatgatgta cttaaatttg gagaaattat aagtaaagat aatggagcat
     2761 ctctaaattt atgctcttac aataaagatt ttttagattc tactagatat aagttagtta
     2821 aaggtagaga gcctagaact tctaatgaat tagtaataga acaaaaagtt ttagacaaaa
     2881 tggaattaga tgtcaaatta aatcaaacta ttgattttca gattgttaat gagcatataa
     2941 ataaaaataa tgaaaattct atttatagta aagataagca atttaaaata gttggtattt
     3001 taagtaaatc tgatgaatac tatgaaaatt taaataaata taaggtaaga gcttttacat
     3061 attcaaacaa tggcaaagat attgttccat atgaattaat aactcatatg ggtacattaa
     3121 aattgaatac aaaagattta gattctcaaa aagtagtgaa tctagggaga aaatataagt
     3181 taacaagtaa tcaattttat ccaaatacag aagtaagtat gtctatgtat atgaaaaata
     3241 tttcaaaaaa aacaacttat tcaataaagc aaagcttact ccctttatta tgtgcaacat
     3301 tagtaatata caatatgttt aacataataa tactagatat gaccaagcaa attggtttac
     3361 ttagagctgt tggagctagc aagagaaata ttagacaggt attttttatt cagagtattg
     3421 tagttttatc tgtaggtata gtaattggtt tacttggagg tatagttttt tcttacttag
     3481 gtctcatgat agtatatgat aaaagtgcag aattaactat aaatacaaac tctataattg
     3541 aatctatcat catgtcaatt atagcagtta tattagcctc tgtagtacca atatataaag
     3601 ctggaaattt atctataatt gatgctataa aaaagacaga taagttaaat tatattccta
     3661 ggcttttgtt gttaaaatca cataaaaaag ttgttggaat tataaatgag atagctttta
     3721 aaaatatatt taggaataag gttacatcaa taataataat aattacgata tcactatgtg
     3781 gggttttatt tataggcaga ctaacttcta caaaattcat atatggaaaa gagtcagatt
     3841 cagctaatat aacaagtaaa tcatatggca attttgatat ttatttggga tattcaccaa
     3901 taaatgcaaa ttatacattt tctaaatatg ataattcttt attaaatgaa ataaaagcta
     3961 ttgaagatgt aacagatgtt gaacccaata tttatttaaa aggatatcta agaaatgctg
     4021 acttagaaga agattattta gatgagctaa aaagaactga aataaataat aataatgagt
     4081 ctacagtttt aattcgaggt tacaataaag agctattaaa taatatagat aaatatatag
     4141 ataaaggcga aaatgtttat agttatacag acagggatta taaaaatgtt ttattggtaa
     4201 ataattttta ttctagaatt aaattgtcaa ataatactca gattataaaa tcacctaaaa
     4261 taggagactt aatagatata aaaattcctg tatataaaga tggagagtat aaatatataa
     4321 atacaaaagt aagaatcgct ggaataatga aaaatagcta tatatcagag caagatggag
     4381 aatctcaatc aggtggaatt caaattatat ttaatgaaaa ggatttaaaa gaattaacag
     4441 ggataagtga ttataataaa gtttttgtga agataaaaaa agaaacagat aaaaaagtaa
     4501 taaaagaaat aaataatata atagaaaaaa gtggattttc agatatagag ggaagatatg
     4561 tacgtaatca ttttggagac cattataata aatcctcata taaattagca atgatttgtg
     4621 tatttggagt tttattaata tcatctataa acattatttt tatagttaga tcaaatataa
     4681 ttgtgaggtt gagtgagata tgtacattta gagcaatagg gatgtccaaa aaaaatgtaa
     4741 aaagaatgtt aataaaagaa aatatgatat atggggtatt aagtatgatt gtagctggaa
     4801 ttatatctag tttcaattat tataaaatag tttctgaaac taacaaactt aatcaacagg
     4861 tatatggtat aaataactct attaaatttg aaattccaat atatgaaatt ctaatatttg
     4921 gatctatatc tgtttttgtt tgtgtcattg cagtttattt ttctaataaa atgataaata
     4981 aacttagtat agtaagtggt ataaaagaaa attaatgaaa tttctaattt aaatatatag
     5041 gaaggaaata aaataaatga atagctcaaa caaatatttt ttagattgta tatgtgaaaa
     5101 aataataaat aataatcaag ttattcttag taataaagta aatggaagat gggttaaaat
     5161 tccaatagaa tgttatgaag ttctaaagta ttcagtagat aatgcattac aacttggtaa
     5221 tttgtatgat gtattccaag aaaatgaaga taaaaaatat tatgaacgag ttatagataa
     5281 gttagagcat ataggacttc taagtgattc tattctaagt gaatttagac tagagagaat
     5341 aaacaaaatt tcattttctc caactaatag atgtaatcta agttgtgatt attgctgtgt
     5401 agattctaaa atagataata acgattattt agatacatct caagtaagaa aagtaataga
     5461 taatatagtt aaattaaatc caaggatatt aaccattaca ggtggagagc cattgatgag
     5521 aaatgatttc ttagagctgt tggattatat aaaagaaaaa tttaaaggta aaataattct
     5581 gtctacaaat gcaactttaa ttaaagaaaa acagattgat gatataatta atggtttata
     5641 tgcaatagaa attagtttag atggttatga tgaattaagt tgtagtgaag taagaggtaa
     5701 aggagtattc tgtaaagtat taaatgtgat tagacttata aaactaaaaa aattcgagaa
     5761 cgtaggtttg tctatggtgg taggaaagaa taatgagaaa gacatagaga aattcaataa
     5821 attaaatgaa gatttaggta caacgcctat aataagaaat tttatgaata ttggtagagg
     5881 taatgagaac tatagcaaat atcttaatga taaacttgat attgattaca tttgcaaaga
     5941 tgattatgtg gatgaaagta gtttatgttc aaatatgtgt aaagcaggtg ttaatcagat
     6001 aagtatagat cataaaggag atgtatatcc gtgtccaaac ttagagtata aggatttaaa
     6061 gatattcaat atattagaat ttaatgaaaa catattagag aggattctta atagagaaat
     6121 ggatatattt aatatatttg ataaattaaa accagtaaat attgagagtt gtaaagaatg
     6181 taaagtaaat ttattttgta caacttgtcc atcgaaaata tatactttaa aaaataacaa
     6241 aaaaatgttt gatgcaaatt gtaaaaaaat gaaaaacatc ttggaaccta tattatggta
     6301 aggattagtt ttatattcaa ggtaatttat tttagaggtg attattaatt gactaaagta
     6361 agaatagcag tcttagacac aggattagat gtaaatgatg atgatattaa tggaaattat
     6421 gtttatgatt caaattttca attgaaagat ttggggggga aggaaataaa tgacataaat
     6481 ggacatggaa cttttgtatc gaaaaccata ttaaatataa gtaaaaatac ccaaatttat
     6541 cctattaata tatttaatga ttctggaaaa acaagttctt taaatcttct tagtacactg
     6601 agcaagttga ctatgtcgga tatacagata ataaacattt cagctgcatc tattaatagt
     6661 atgtatgaaa aagaattaga aaaggtttgt aatattttaa atagtcaagg tagatttatt
     6721 atttgttcaa aacataataa atatgaagat aaagaaagta ttcctactat atttgaaagt
     6781 gttattggtg tagtaggtaa tgaaaatata tataaaaata atcaatatat atacgaacca
     6841 gaaaataaga ttcaaatgta tgcaaattca aaagataatt ttattaaatt taaagataaa
     6901 gtaactcatt ttggtaaaaa tagtaaggct tgtgcagtgg caactggcat tgttgcaaat
     6961 atattaaaaa acaatccata tattaagttt aatgaattgg aagaaatact tattcaaaaa
     7021 agcaaaagta aagaagattt aaaaatagaa aacttgaaca ctccaagtta taaatctaat
     7081 ttgataagat tggatatagc aaggaaaata ataaatgtga taaatagaaa cttttctaaa
     7141 ataaaaatag actttgaatt tgtaagtgaa tattctttat ttaacaattt tacatgtatt
     7201 ggaaattata atgcatataa ttttttaact attttaaata aagagtttaa tattagtatt
     7261 gactacaaag aagtatactt atatcaatta gagaatttag atatggcagt agatcttatt
     7321 tacgaaaatt taatttaaaa atatatgttt tttaaatggg aatggagata cgttatgaaa
     7381 atacttaagg taaaagattt aaaaaaaata tatggaaaat atgagaatga agttagagct
     7441 cttgatggta ttgatttaga tatagagcca cataaattta cagctattgt aggtgcaagt
     7501 ggttctggta aaagtacact acttcactgt gttgcaggct tagacaagcc aactttaggg
     7561 aatgtgtact tagatgatgt agatttatat acattaaatg atgataagct gtctgaaata
     7621 agaagacagg aatttggctt catatttcaa agttacaatt taataccagt tataaatgta
     7681 tatgataata tagtacttcc tgtgttgctt gatgggaaaa aagaagataa agaatatgtt
     7741 atgagattaa ttaataagtt ggggattgaa aaacaaataa agaaatatcc gaatgaacta
     7801 tcaggaggtc aacaacaaag agttgcaata gctagagctt tagccaatag accgtcagta
     7861 atatttgcag atgaacctac tggcaactta gatagtaaga ctacaaatga agttatgaat
     7921 atgcttgaat ggtgtgttaa agagtacaaa caaacactag ttatgataac tcataatgat
     7981 aatattgcaa agacagctga tagaattata actataaaag atggaaaaat agatgaaaaa
     8041 aactattaat aataatttat ttagaatact aaatataata gtatataaga ataagactaa
     8101 gttgatagta tcatttataa tggtttttat aattgctggc gtaaatattg taatacctca
     8161 actaattaga tgtataatag atgatggaat aggaaataaa aatatttttc tgcttataaa
     8221 tttaatattg atatatattt ttataaggat attatcagct ac
//
LOCUS       DAYATN010000154         8165 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.155, whole genome shotgun sequence.
ACCESSION   DAYATN010000154 DAYATN010000000
VERSION     DAYATN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.155"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..838
                     /locus_tag="ACGI8A_003247"
     CDS             <1..838
                     /locus_tag="ACGI8A_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFH0181226.1"
                     /translation="KGTFRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMG
                     IFLPIVCKNLNKKTIIIVIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYI
                     NMNKLFPNDINRFKAIIGTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENR
                     EIIKDINKDTVDIVGTFESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKL
                     VSYIITKESKMDIVKYEEFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGL
                     YE"
     gene            953..1738
                     /locus_tag="ACGI8A_003248"
     CDS             953..1738
                     /locus_tag="ACGI8A_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181227.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            2030..3349
                     /locus_tag="ACGI8A_003249"
     CDS             2030..3349
                     /locus_tag="ACGI8A_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0181228.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(3424..3852)
                     /locus_tag="ACGI8A_003250"
     CDS             complement(3424..3852)
                     /locus_tag="ACGI8A_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181229.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(3943..5319)
                     /gene="hydG"
                     /locus_tag="ACGI8A_003251"
     CDS             complement(3943..5319)
                     /gene="hydG"
                     /locus_tag="ACGI8A_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HFH0181230.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(5320..6405)
                     /gene="hydE"
                     /locus_tag="ACGI8A_003252"
     CDS             complement(5320..6405)
                     /gene="hydE"
                     /locus_tag="ACGI8A_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HFH0181231.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(6441..7649)
                     /gene="hydF"
                     /locus_tag="ACGI8A_003253"
     CDS             complement(6441..7649)
                     /gene="hydF"
                     /locus_tag="ACGI8A_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HFH0181232.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTQKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(7664..7915)
                     /locus_tag="ACGI8A_003254"
     CDS             complement(7664..7915)
                     /locus_tag="ACGI8A_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HFH0181233.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         2918 a         1123 c         1184 g         2940 t
ORIGIN      
        1 caagggaacg tttaggaatg taaatttaat tccattttat acaatatatc aatttatagt
       61 agatagtaat cttgacttta tgaaagctac tgtaaatatt atagaaaata taggtatatt
      121 tattcctatg ggaatttttt tacctatagt atgtaaaaat ctaaataaaa aaactataat
      181 tatagtaatt atacttgtta gcctagcatt tgaattaaca caatatattt ttgctcttgg
      241 aagtagtgat atagatgatg ttattttaaa ttcattaggt ggcataattg gaattaatat
      301 ttacataaac atgaataaat tatttcccaa tgatattaac agatttaagg ctataatagg
      361 tactagttta gttttaggag ttataggatt aggagttatt tctaaaaatt atcataactt
      421 actaactttt aaatttagac ctgatagaaa aatatcaaaa atattaattg aagaaaatag
      481 ggaaataatt aaagatataa ataaagatac agtagatata gttgggacat ttgagagctt
      541 taaaaaagga ataataacta ttaaagcagg ttctaataac aaagtaaaga gaccagagaa
      601 tttaattgat tctgatggaa atataagaat ttatttaaat gaaaatacaa aattagtatc
      661 ttatataatt accaaagaat caaaaatgga tatagttaaa tatgaggaat ttgatgtgaa
      721 gaatttagat ttattaagga gatatgatac tattaatgtt tggattgata aagaaaatca
      781 gccaaaggat aaacatggta ttatggctag taaattatta attggattgt atgaataaaa
      841 tattaaacaa gaataaaaga ataacaaata taaaaaacga aaaaactatt tctataatta
      901 tttttacgtt gtgcataaaa taagaacatt agtgaaggag tagagataaa ttatggagat
      961 aataaaatat atcttgtact tttttatata ttcattttta ggatggacag tagaaagtat
     1021 aggatgttct atagcaagca aaagaataat caacagaggt tttttaaatg gtccaatatg
     1081 tcctgtttat ggttttggag cggtaattgt gatatcacta ttgggaagat ttaataatgt
     1141 agtaatagta tttctattag gaatgatttt aactacgata ctagaatatt ttacaggatt
     1201 tattttagaa accttatttc atgctaaatg gtgggattat tctgacagaa aattcaatat
     1261 aaagggtaga gtatgtttaa aaaatgcaat atactttggt gtaatgtctg ttttaataat
     1321 aagatttata catccattta taaaatattt tgtaagtata attccatata gaatattaat
     1381 ttcaatggct ataattacaa cactttggac tatacttgat ttgattgtta ctattataac
     1441 acttaaaaaa ttagatataa agttaaactt actagacgat ataattacag accttaatga
     1501 tataaatgtg aaattggata aatttgatag aggagaaata caagctttat ttaaaacaat
     1561 aaataacgac gaattagaag ttagagaaaa aataaacaaa ataaatagta agttggaccg
     1621 tatagagtct aatattattt tacaaaaaag agttataaaa gcttttccac atataaaaca
     1681 taaaaaacaa caagagcaac tagaacattt taaaaaaatt attaatgaga aaaggtaatt
     1741 atacaattta atgaagacga atagaataat ggtatttata agatgatttt cagaggactc
     1801 tataaaaata gaatagagtc ctttattttt tgaaaaataa tgtttatttg ataaaaataa
     1861 aatatattgt ttctgaataa caaatatttt gaaaattata aaaacatata ttgacaattt
     1921 gtgtcaaaaa tactaacatt aagtatataa agaattttat atgagaatat ctttataaat
     1981 cgcaataatt ttaaaacata aatttattta aagaggggat gatttgccaa tgagcgagat
     2041 aactagtcaa atgataagta cagaagaaaa aaaatatgtt gctaaaactc aaaagatacc
     2101 ttattatcca gtagcattta aatcaggtga tggtgcaatg ctatatgatt atgaaggtaa
     2161 tgaatatgta gattttttag caagtgcagg ctctgcaaat gtaggtcatg gtaataagga
     2221 aatctcacaa gcagttaaag aacaaatgga tgatataaca caatatacac ttgcatattt
     2281 ccatagtgat cctcctgtaa aattagctga gaagcttgtg gaaatagccc ctggagataa
     2341 tgataaaaaa gtactataca gtgcaactgg ttctgcttgt atagatgctg ctataaaatt
     2401 agcaagagga tatactggaa gaacaaagat aatatctatg tgtgaatctt atcatggaag
     2461 tacttatgga gctatatcta tatctgcttt aagtacaaat atgagaagaa aaatgggtcc
     2521 attgttacca gaagtttatc acttccatta tcctgataaa aatagaacag ctaaagaatg
     2581 tttagatgag atagaatatg cttttgcaca ctatctacca gcagaagagg tagctgcaat
     2641 atttatagag ccaattgcag gagatgcagg aataatagtg cctccagtag agtgggtaca
     2701 aggtttaagt aaaatatgta aagaaaatgg aatattactt gtaagtgatg aaatacaaca
     2761 aggtatggga agaacaggta agtggtttgg tatagaaaat tttggtgttg aagcagacct
     2821 tatagttctt ggaaaatctg ttggaggtgg attacctcta ggagcagttg ttggaagaac
     2881 agaaataatg caaagtcttg atgcaccagc acatttattt actttagctg gaaacacaac
     2941 agtatgtgtg gctgctttaa aatctataga gataattgaa aaagaaaact tacttcaaaa
     3001 gagtatagaa atgggagatt atattaaagc aggatttgaa aaactaaaag aaaaatacga
     3061 tataattgga gaaataagag gcataggatt atctatagga gttgatatag ttaaaggaaa
     3121 aggttctaat gaaaaacatc cagatgctac agctaagata tgttatagat gtatacaaac
     3181 tggattaata atgatattct taggacaatc tactttaaga gtgcaaccac ctctagtaat
     3241 aactaaggag caagtagata aagcaatgaa catcatagat tctgcaatag atgattattt
     3301 aaatggtaga attggtgatg aagtatatga agtaactcaa ggttggtaaa ataaatatta
     3361 aatgaaaata ggagttatct caattttaga aaattatttc tgaaatgaga taactctttt
     3421 aagttaccca caaataagaa aattgtttga ttcattaaag actatatttc ggatgtcgtt
     3481 tttgtgagta tcagagacag tattacgaga ataattacct ttattcgtac tatctgttaa
     3541 tttagttata attaagatag ctaaaagaat aagaattact gtgtataaag caataacata
     3601 tggttttctt ttatatttgt atttgttatt gaaaaaagaa tggccataat tatttttatt
     3661 tactttagat gtatttgtat tctttttctt gttacctttc tttttatcat ccttttttct
     3721 atttttaggg taaggaatga ctttgtgtgt attatcaagt ttgatatttt tatttttttg
     3781 aatattgttt tccaggctag cctttttttc tccaaattga atagttttat tggtattgtt
     3841 gttatctttc aatgttaatc tccctccctt aaactttccc tttagataat attactacta
     3901 acctatggaa tatacaataa ataatatata agtaataaaa ttttataggt ataaatctct
     3961 ttctccacgc tctaaggcag tcatattgtt agatactatg tttcttatat cttctctttc
     4021 tatcttacct atttcttctg cgataagttt tttacccata attttaagtt cttcatctcc
     4081 ataatcgatt aaaaattcat ttaatgtagt aagagcattt ggtgtacata cattatggat
     4141 ttgtcgactt ttagctaagc tcatgaatct atcgccagtt ctacctttac gataacaagc
     4201 tgtacaataa ctaggtatat atccatcagt aatcagttct ttaagcacct caacagggct
     4261 tctatggtct ccaacttcaa attgttctgt attgtcacca tcttcatatg ctttatatcc
     4321 tccaacacct gttagagaac ctgcactaat ttgtgataca ccatatttta gtaactcatt
     4381 tctcatttca gcagtttctc tagtggacat tatgattcca gtaaaaggaa cagccaatct
     4441 agttatagct actatttttt tgaacatatc gtcagaaact aagtgaggaa aatcttctaa
     4501 gctcatacct tcagcttttt tcaatctagg gaaagatata gtatggaaac ctacaccaaa
     4561 tttttcctct aagtgttcgt tgtgcatcat aagtcctaga acttcaaatt tagggtcaga
     4621 taaaccaaat aaaactcctg caccaacatc atcaacacca gcttccatag ctctatcaaa
     4681 agctgttaaa tggtaataat agtcattttt tatagattga ccatgcattt ttataaatgt
     4741 tggtttgtga taagtttctt ggaataatat ataagttcct attccttttt cttttagtaa
     4801 tttatactca tctacacttg tagctgctat gttaacgttt acccttctta tatttccgtt
     4861 ttcattatat gttgaataaa ttgcatctaa gcaatctaag atgtaattta tatcacaatt
     4921 tttagggtct tcaccagctt ctagagcaag tcttttatga cccatttttt ctaatatttt
     4981 aacctcttcc tttatctctt ccatggtaag tttttttctt ttaaatttgt tgcatttact
     5041 aaaaccgcaa taaacacatt catttacaca gtagtttgat acataaagag gggcaaataa
     5101 aaccactcta ttaccatata tttcattttt tatttgccca gctatactaa ataatctatc
     5161 tagttgtttt ttatcttcta cttcaagaag tgtagctata tctttataac taagtttttc
     5221 ccttctatca gccttatcaa gtaccttttc aatatcatca gaagttgaat tttttgcatc
     5281 ttctaataaa gaattaataa gttcatggtt tatgaacatt tacaaacgcc tccattcaat
     5341 attatcaccg cgagaatagt caacttcaag tcctaatgac ttgatgtttt cttctaaact
     5401 ttggtgatat tcacaagctt cttgaccagt aaaggctttg ttatcgtaaa gagaatattg
     5461 ttttctaaat tcttgaggag aaaggtttgg cataattaca ttgcaaccag ctaaaagacc
     5521 tgcatttcta ccactagggt ttatagatgc caaggctgtt gtagcgggta ataaaacttt
     5581 tggaagtaga agtcttgtaa ttgaaagcat aagtaatgtc ttctctaaat ctccatgttt
     5641 ataatcctta aaaattgtat catggtgtgg aataaaaggt cctattccaa ccatatgagg
     5701 atttaactct tttaggtata ataaatcacg aacaaggtct tcgttgttct gaaatggtga
     5761 ttcaaccata aatccagcac ctacctgata accaatttct ttaaggtttt ttaggcattc
     5821 ttttctagtt ctaagctcaa tgtttggcgg atgtaatttt ttataatgac tgtctgtagc
     5881 agtttcatgt cttaaaagat atctatctgc accacataaa aagtattttt tatacgaatc
     5941 ataactcttt tctcctagtg atagagttat ggcacaatcc tcatatttat tttttatact
     6001 tgaaacaatt tcacatattt tatcatcagt aaaatatgca tcttcaccac cttgcaaaac
     6061 gaatgttctg tatcctattt tataacctat atcacaacat tcaagtattt catcaagtga
     6121 aagcctgtat cttatgatat ttttattaga agattgtatt ccacaatagt agcaattatt
     6181 tttacaatag tttgtaagct ctataagtcc tctcaagtag actctatttc cataatgttt
     6241 atttctcatg tcgctagctt gtttataaag atagtttctt aaattttgtt cgaagttata
     6301 ttcctcattg tcgatatttt ctattaaata tagtagttca tcaaaactta atgaattaat
     6361 gctgtaaagt ttatctataa agtatttaat cttattttta gtcatattca aatatgtaat
     6421 tattaagtat agttaaattt ttaataattg aaaagttcaa gtgatctttc caaaatacct
     6481 tttacatatg ctattaagac accatagttt acaataggta cattgaaaga ttttgccttt
     6541 tctattctag ataacattcc agctctattc atcatacaag ctccacagtg aactacaagg
     6601 gcatatttat ttatatcttc agtgaaggat actccagaac taaattcaaa agtaatgttt
     6661 ttgccagttt tctttctaat catattagga atttttactg ttccaatatc atcagtttgt
     6721 ctatgatggg tacatccttc ggccatcaag attttatcac catcttttaa attttctaaa
     6781 gcataagctc cattaatcaa ttctttaagg tctccctttt gtctagcaaa caatatagaa
     6841 aaagatgtaa gaggaatatc tttttgtgta tctttatcta cttgtggaaa tacttgagag
     6901 tcagtaataa caagttttgg ttttttgcct aaattagata gagtttcttt taaactatct
     6961 tcttttgtaa ctatagaaat tgcaccatta tccaaaatat ctcttataac ttgctgttga
     7021 ggaagtataa gtctaccttt tggagctgct ttatctattg gaacaactaa aacaactaaa
     7081 tcatttggtt caattaaatc tgataccaat ttgaactcag tgctatcctt aggcaaaact
     7141 tttattattt cattttttaa attttcaatt ccaattttat ctgttgatga gacagaaaca
     7201 actggacatt taactttatt tttagttaag tttaaaattt ctgattgatt cttaatagta
     7261 tcaattttgt taagaattag aatatgaggg atattcttat cattaaattt ttcaattagt
     7321 gacaaatctt cttgtgatat accaatttgg caatccacta ctagtaaagc tatatctgtc
     7381 ttttctaaaa catctagtga tttacctatt ctaagttcac caagttcacc aacatcatca
     7441 agaccagcag tatctataag aacacaaggt cctataggaa ggatttccat tggtctaaat
     7501 actgggtctg tagtagttcc agcaatatct gagactattg cagcactttg atttgttata
     7561 gcatttatta tgctagattt acctgcattt cttttaccaa agagacctat atgtactctt
     7621 accgattgtg gagtagaatt tagactcatt tgtactcacc accttaaact tctttttttg
     7681 atatactagt ttttacagat acatgaggta tgttgcccaa tttaccagtc aaactattaa
     7741 tagtgtccat ttcgccaaca actgctatac atacgattga gacaccttct tcttcgcaag
     7801 gaatgcccat tcttccttta ataatacctt taaaacctga tacaacctca ttgaatttaa
     7861 attgacattc ttttggttct tccaaaatag cacttataac tgcaaccttt ttcataaata
     7921 tcctcctaac tcagaaaata tgtcttaaac aaatccaaaa tcaaaaaact tttctttgta
     7981 aagaaaagtt tctacaaaag aaaagttatt aattacacaa gtataatgtg ctatattaaa
     8041 ctatttcctt acagattaga taactctttt ctggtagggt tagcttgtaa aaatttaatt
     8101 taacaacaag taactgaaga aaatttttat acaatttata taaattataa taaaaattaa
     8161 ctaaa
//
LOCUS       DAYATN010000155         8037 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.156, whole genome shotgun sequence.
ACCESSION   DAYATN010000155 DAYATN010000000
VERSION     DAYATN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.156"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(70..2481)
                     /locus_tag="ACGI8A_003255"
     CDS             complement(70..2481)
                     /locus_tag="ACGI8A_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFH0181234.1"
                     /translation="MRKYKLKKLSKLLVLLTVCFLIVSTIPVSAEKNKILTGVETAEY
                     SESYLQYLEDVKNGDIAKYNGVIPTPYEMEGTTLKTNVRSSLPASYKSSVSYDPRKLD
                     LTTPAKDQGKLNTCWAFSGMSTLEAYLKLKGYGTYDLSEEHFRWWSTGGIHGWNLTDM
                     TGSSNVTAIGYLTAWAGPKLEKDIPYNFKSEDEGATRPQNMDTAPTQFNVTDVVRISK
                     DKTSVKNAIMQYGAVTSGYAHYSAYLSDDENSYNCNDKSEPLNHSVSIVGWDDNYSKD
                     KFKPSVRPESNGAWLVKSSWGEFNSEKGFFWISYEDKTLLKDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            2656..3072
                     /locus_tag="ACGI8A_003256"
     CDS             2656..3072
                     /locus_tag="ACGI8A_003256"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFH0181235.1"
                     /translation="MEENFLILKKHLETILYLFFGALTVLINIITYLFFTRIINFTFL
                     LSNSLSWSIAVLFSYITNKFFVFKSEKTEFLFLLKEFVSFLSCRLFSGFIEILLMYLM
                     IDLFSLNDILVKIGTNIIVIILNFVFSKVVVFKHRN"
     gene            complement(3137..5254)
                     /locus_tag="ACGI8A_003257"
     CDS             complement(3137..5254)
                     /locus_tag="ACGI8A_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFH0181236.1"
                     /translation="MQISKKIISLLTAAFLSIVLPSNVVNASNKDSLIGSGRWETAIK
                     ISQNGWLRSDNAVLVNDNSIADALSATPFAKLKDAPILLSGSKELDFRTKSELKRLGV
                     KNVFLVGGSNALGVNVENELKSEKITVERIFGNDRYETSLKLAEKLNNEKDISDIIVV
                     NGEKGLADAVSVGSIAAQNYMPIILSDSNLGVEVANKFIKAQNIKNSYIIGGLNSISN
                     EIEKNLPNSKRISGNNRNETNANVIKEFYKETDLRNAYITKDGMKKQSDLIDSLSVGV
                     LAAKNNSPVVLVGNKIDSIQKDVLNTKIFSTVTQVGGIGNENVYKDILDMQEETKYTV
                     ETIEELDSALKKVDANDIIKFRPDKKISESFKLETEKAIKVDLYGEYSQSITINMPNG
                     ELNNTGDISKTLILKDIKDGTLTNSGYINQMDISDDNGCKIENENNGQIWIVTIFSSA
                     KDVNIRNDGTITKIDNDSSSTNIRNSGKIDTVSGNKEPGISGNKPNTNNTDKDDDSDT
                     AAGLSPKISACSPLENNQIMLTVPQKPKNSNYKIYYRVLSYKPSAMRVGDRININDWD
                     LVEDTEPLKVNAKNGSYIEVVEIDYTRKKVSRWGRAGATDDGFKIVANGIKSTVSVDE
                     NSKVTISVTGYNGNKVYYRIKTTKPEAMNVGDEIDSSWKSTSNSDIILEGNKVNGLYV
                     ELASGENNKVEKWGATDKIEIVS"
     gene            complement(5285..5992)
                     /locus_tag="ACGI8A_003258"
     CDS             complement(5285..5992)
                     /locus_tag="ACGI8A_003258"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFH0181237.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAKEIILKYLEEYPEAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDDLELNKTQYVHAP"
     gene            complement(6099..7970)
                     /locus_tag="ACGI8A_003259"
     CDS             complement(6099..7970)
                     /locus_tag="ACGI8A_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HFH0181238.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRGLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
BASE COUNT         2323 a         1449 c          916 g         3349 t
ORIGIN      
        1 ttatatttat aaacttctat tgaatatttt ttaataataa aatgtatatc cttactatct
       61 ttccattcac tattttccta aaagagtatt tagttcatta aaagcatttt catttccatt
      121 tccaccaact ctaataggag tttctatttt cttagactta agtacatttt tttgactttc
      181 atctaactta tttccaacta aaactactgg tgaatcagtt ttagcaccta atgctcctac
      241 tgctagtcca tctatcaagt ctccttgact ctttgaacca tctttaacaa caaaagcata
      301 tttcaaatct gagctaggat agaatttatc tattatctta gcattagttt catttctatt
      361 acttcctgca agtcttgttg gatttgaaag cttactttct aaatttgatg ataaagtagc
      421 tgttccacca attatataag acttagttat tttagatgaa tttaaaaact catcagcacc
      481 ttgcaactca ctcttttcat tagtaagtat tattggcata ttgttatcag cagctgctgc
      541 accaacactt attgcatctg caagtccatt tacaccattt gccactgcaa cttgtgaaac
      601 agattttata ctatttaact tttgagctag agatatactg gtcttgtatc tatcactacc
      661 tgaaattctt tctactgata tacccatatt actaagttga gtttgtacat tcttgcttaa
      721 agaattttct ccacctatta gataaacttt tttagttcct aatctttgta actctgcctt
      781 agtttttgaa tctaagttat cttttcctgt caaaagaatt ggagagtcaa ttgctgctgt
      841 aaatggtgta gctgttaaag catccactat tgcatctcca tttactaata ctgcattttg
      901 tgaagaagtc catccttttt gactaacttt aactgctgtt tcatatctat ctgctccagt
      961 taaagattct cctgaagtag cttgtccaga agatgttttt gttatagctt ttataacaaa
     1021 gttacaagga gaaaattcac tatatgtatt tatatcttca aatttattgt tttgaagtat
     1081 gtagctttca cctaagtttg ctttagcctc atatagatag tatccatcta tatcagtttc
     1141 atatgctaaa gttgattttt ctctattagg atttgctgtg ttgtctataa ctactactat
     1201 tgctccttta ccttttggta agctgtaaga attagtaggt acatttatgt atccagaata
     1261 tgatactgtt ccacttgcaa gttttgtcat tgaattgttt tgaggaactc catttactac
     1321 tggtgcataa tatacttcat attttgctcc tacagaatct gtttcaaaca taacagagtc
     1381 aagtttttca gaatctctgc taaaatcaaa tacatttgct gctgttactt tatttgacat
     1441 tatcttgcta agaccagcat attcaagttg gtacattttt ttatcgctat ctggttttga
     1501 tactgatttc attgcgtagt tatctgtatc ttttaaaaga gttttgtctt cataagaaat
     1561 ccagaaaaag cctttttctg agttaaattc tccccaacta ctttttacta accatgctcc
     1621 atttgattct ggtctaacac ttggtttgaa cttatctttt gaataattat catcccatcc
     1681 tacaattgaa acagaatgat ttaaaggttc acttttatca ttacaattat acgaattttc
     1741 atcatcactt aagtaggctg aatagtgtgc atatcctgat gtcactgcac catattgcat
     1801 tattgcattt ttaacagaag ttttatcttt actaattctt acaacatctg ttacattaaa
     1861 ttgtgtaggt gcagtatcca tattttgagg cctagttgct ccttcatctt ctgatttaaa
     1921 attatatgga atgtcttttt ccagcttagg tcctgcccag gctgtaagat atccaattgc
     1981 agttacattc gaactacctg tcatatcagt taagttccat ccatgtattc ctccagttga
     2041 ccaccatcta aaatgctctt ctgaaagatc ataagttcca taacctttta atttcaaata
     2101 tgcttctagt gttgacattc cagaaaaagc ccaacatgta ttaagttttc cttggtcttt
     2161 tgctggtgta gttaaatcta attttcttgg atcatatgat acacttgatt tatatgatgc
     2221 tggaagacta cttcttacat tagttttaag tgttgttcct tccatttcat aaggagttgg
     2281 tattactcca ttatattttg ctatatctcc attttttaca tcttccaagt attgaagata
     2341 tgattcagaa tactctgcag tttctactcc agtcagaatt ttattttttt ctgctgagac
     2401 aggtattgtt gacacaatta aaaaacaaac tgtcaacaac accagtagct ttgacaattt
     2461 ttttaattta tactttctca tatttacccc cttgtatgta tttaattttt attatatcag
     2521 atttctaaat ctaggtcata ttttattttt ataaaaatag gaattataat tatacatata
     2581 taaaatacta ttttatactg catatttatt gattaatatg tggtatacta attacataat
     2641 aatatatacc aaataatgga ggaaaatttt ttgatattaa aaaaacatct agaaacaata
     2701 ttatatcttt tttttggtgc tttaacagtt ttaataaaca ttattactta cctatttttt
     2761 acaagaatta ttaattttac ttttttatta tcaaactctc tatcttggtc tatagcagtt
     2821 ttattttcat atataacaaa caaatttttt gtattcaaaa gtgaaaaaac tgagttttta
     2881 tttctattaa aagaatttgt atctttttta tcttgtcgtt tattttctgg ttttatagaa
     2941 atattactta tgtatttaat gattgattta ttttctttaa atgatatctt agtaaaaata
     3001 ggcactaata taattgtgat aattttaaac ttcgtcttca gcaaggtagt agtttttaag
     3061 catagaaatt aacattatta tatttatctt tatgcagaat aaggagttgc ctaaaaaaaa
     3121 caactcctta ttagctttaa gaaacaattt ctattttatc tgttgctccc catttttcaa
     3181 ctttattatt ttctcctgat gctaattcta cataaagtcc atttacctta tttccttcta
     3241 aaattatatc actattagaa gtacttttcc aactggagtc tatttcatct cctacattca
     3301 tagcttctgg ttttgtagtc tttattctat agtaaacttt attaccattg tatccagtta
     3361 cactaatagt tactttagaa ttttcatcaa cagaaacagt tgattttatt ccatttgcta
     3421 ctattttaaa accatcatct gtcgcaccag ctcttcccca tcttgaaact ttctttcttg
     3481 tataatcaat ttcaacaact tctatatatg atccattctt agcatttact tttaatggtt
     3541 ccgtatcttc aaccaagtcc caatcattta tgttaattct atcaccaacc ctcatagctg
     3601 atggtttata tgacaaaact ctataataaa ttttataatt tgaattttta ggtttctgag
     3661 gtacagttaa cataatttga ttattctcaa gaggagaaca tgcacttatc tttggagata
     3721 atcctgctgc tgtatcacta tcatcatctt tatctgtatt attagtgtta ggtttattgc
     3781 cacttattcc tggttcttta tttccactaa cagtatcaat ctttcccgaa tttcttatat
     3841 tagtacttga agaatcatta tctatctttg ttatagttcc atcatttctt atatttacat
     3901 cttttgcaga actaaatatt gttacaatcc atatttgtcc attattttca ttttcaatct
     3961 tgcaaccatt atcatcagaa atgtccattt gatttatata tcctgaatta gttaaagttc
     4021 catcttttat atctttaagt atcaatgttt tacttatatc acctgtatta ttcaactctc
     4081 catttggcat atttatagtt attgactgac tatattctcc atataaatca acctttatag
     4141 ctttttcagt ctctaattta aaagattcac ttattttttt atcaggtcta aactttataa
     4201 tatcattagc atccactttc tttaaagcag aatccaattc ttcaattgtt tcaactgtat
     4261 atttagtttc ttcttgcata tccaatatat ctttatagac attttcattt cctattccac
     4321 caacctgagt aactgtacta aatatcttag tatttagtac atctttctga atagaatcaa
     4381 ttttatttcc tactaatacc actggagaat tgttttttgc agccaacact ccaactgata
     4441 gtgaatctat taaatcactt tgttttttca ttccatcctt ggttatatat gcatttctta
     4501 aatctgtttc tttataaaat tcttttataa cattagcatt tgtttcattt ctattatttc
     4561 ctgatatcct cttagaattt ggtaaatttt tttctatttc attagaaatg gaatttaatc
     4621 ctccaataat ataagaattt tttatatttt gggcttttat aaatttatta gctacttcaa
     4681 ctccaaggtt tgaatctgaa agtataattg gcatgtaatt ttgagctgct attgaaccaa
     4741 cacttacagc atctgctaat cccttttctc catttactac aattatatca gaaatatctt
     4801 tttcattatt caatttttca gccaatttta aggatgtttc atatctatca ttcccaaaaa
     4861 ttctttcaac tgttattttc tctgatttta attcattttc tacatttaca cctagtgcat
     4921 ttgaacctcc tactaaaaat acatttttta ctccaagcct ttttagttct gattttgtcc
     4981 taaaatccaa ttctttactt ccagatagta aaataggtgc atctttcaat ttagcaaatg
     5041 gagttgcaga taatgcatct gctattgagt tatcattcac taatacagca ttatcagatc
     5101 ttaaccaacc gttttggctt atttttattg ctgtttccca tctacctgaa cctattaaac
     5161 tgtctttatt agatgcattt accacattac taggtaaaac aattgataaa aatgctgctg
     5221 ttaataaaga tattattttt tttgatattt gcattaatta tctcctccta atttaaatat
     5281 aattttatgg agcatgtaca tactgtgttt tatttaattc taaatcatca aaatgagatt
     5341 ttactgaatg gtatcctata gcataatgtc cactttcagg attccatttc ttatatgcat
     5401 tcatcgcact taataacttc tcctcttgac taggtaatac cttccagact tctacatttg
     5461 gattaacttt tttaaaatcc ttatagtcat atggttctac ataaaatctt aaatctttta
     5521 tttttccttc tctatataac tctagtgcag cttctcctaa tgctctatga tcttcatgta
     5581 ttccactagc tttaaacgga gtaacagttt ttacttttgc ttctggatat tcttctaagt
     5641 atttaagtat tatttccttt gccttttctt tgcttaactc accatcatga gctctattat
     5701 cttctatatg tatattactt tcttttacac ccattgcttc acagctatcc ttaaattctt
     5761 catctctatc ttttgaaaat tcactttctg ataaagagta tttatgaaca tctttaatat
     5821 gataagaaca tgaccctcca tcatccaaaa cttttcttat tatagattta ctaccatctg
     5881 tgcacaatat gacatgtgta tcactttttt caacatgttc aattatagca cttcccatac
     5941 ttaaggtttc atcatcttga tgtggtgtaa aaaacaaagt tgcatctacc actactacat
     6001 cctcctactt tataaaatgt tataatttac aataaatagc ataattatag aaaaagattc
     6061 ctgtattaat taaggaatct tctccacagt ataagttatt accaacctag tattttagct
     6121 atcttttgta ttactgtcaa ttcaactcca ttacctattt gagttacttc tgcaccaaga
     6181 tttaattttt tactaagtgc attcttttgt gagtctgtta tatcagtttt agctagaagt
     6241 attggacctt tacctgctgc tagaggtcct gctgccaatg catctactag cataccatta
     6301 tttccatatc catcttttgc tatatatagt ttatctaatt tgccataata tgtttctata
     6361 actttagcat tagtatcgtg tcttgtttct ccttcaactc tttgaacttt tctatcttta
     6421 tcaatatctg caagttgatt ttcaatttgg ctagatacat tattagagcc accaactatg
     6481 ccaatctctt ttccatccat caatttgata gcatctgctg atagatcatt ccaaccattt
     6541 actattatag gagccttaag ttcagcagct ttagcagcta tactcatagc atcagcctcc
     6601 ccttttgcgc ctactacaaa tgcatcttta aaagacccca tagcttttgc tactgctaca
     6661 gaagtcatat gtctatcatc tccagctagt ctttcaacat tcttagttac tgattctaat
     6721 tgctttttag ctgtatttga tactgctgat tctccaccta ctatatatat tttagctgat
     6781 gggcttttct taattatatc ttttatataa tctaaagtta cttttggtat ttcattatca
     6841 gatgctaata atataggtgc ttttttagat tgagcaagtg gtgtagctgc taatccgtca
     6901 actagtttat ttgaattaac tataactata ttgtcagtat attttgtttg ttttgctata
     6961 gccactgcag ttgcatatct atcatctcca gctatcaatt taacctttga attaccttct
     7021 aatgcagctt tggcatcttt taaatctttt tcattggtac tttctacagt aagctctgtt
     7081 gcttttccag atgcatcagt atacttaata gtaaatttat attttcctgt ttctgaatct
     7141 tttgctgaaa tatcactaat tgactttgat gtaccatttg ctaccatatt acctttaaca
     7201 gtcaatctat atccatcata taaatcttgt ggtgaaactg tatttaggtc actatttttt
     7261 atagttattt cggccaattg gacatcatca atatttttag ctcctgctac acctagttta
     7321 tcgattttat ttactctaaa tccttctgca gaagggtcta aatttgttcc agttgaatca
     7381 actggatttg ctgttaaatc aactttttca tcaccaatac ctacatatat agttttagaa
     7441 gttactgttt ctgttttatc tcttaaagtt ataactaact tatctttagc actatcatat
     7501 gaagctttta catctgcaac tttataaatt ccattggttt cagttttagc tttttctgtt
     7561 atagaattag cctcagcgga aaggtctgct gaattttcat atttttcagt tgcttctgca
     7621 actacttcat ttgcactatt tgttatatgt cctttatctg ttatagttac aataagttct
     7681 ttattttctc ccaaagcatt tactaatttc tctaattcat ctatattagt tattattttt
     7741 aattttgata aagtctcacc aacttttatt tcatatattg gttgtccaac ttgagaacct
     7801 cctgtatact tttgagacat taaacctcta actttcgaaa ctaattctgt agcctctttc
     7861 ttagttattg tttctttttt tacctgtgta gtttctgcag caaaaactgg agctactgat
     7921 gtacttatca ttgcagcagc cataattaca gaaagatttt ttttattcat ggtattttcc
     7981 ccctaaaaaa ttcaaatttt tattattttt tccttattta ccaattataa tatatat
//
LOCUS       DAYATN010000156         7981 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.157, whole genome shotgun sequence.
ACCESSION   DAYATN010000156 DAYATN010000000
VERSION     DAYATN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.157"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            397..2631
                     /locus_tag="ACGI8A_003260"
     CDS             397..2631
                     /locus_tag="ACGI8A_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0181239.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2662..3507
                     /gene="dccA"
                     /locus_tag="ACGI8A_003261"
     CDS             2662..3507
                     /gene="dccA"
                     /locus_tag="ACGI8A_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP diguanylate cyclase DccA"
                     /protein_id="HFH0181240.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3747..5480
                     /locus_tag="ACGI8A_003262"
     CDS             3747..5480
                     /locus_tag="ACGI8A_003262"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0181241.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5806..6292
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5938..5966
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6822..6926)
                     /locus_tag="ACGI8A_003263"
     CDS             complement(6822..6926)
                     /locus_tag="ACGI8A_003263"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFH0181242.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7551..7796
                     /locus_tag="ACGI8A_003264"
     CDS             7551..7796
                     /locus_tag="ACGI8A_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181243.1"
                     /translation="MNQKKIKISIKMVIIGIFSVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIIFANCIMFSNILLEIFLIYICRKFRKI"
BASE COUNT         3201 a          952 c         1053 g         2775 t
ORIGIN      
        1 ttaatatggt attaaattat gtataaaaag ttaattatga ataaaataat tatgcctttg
       61 aaaaatcatt aaacttaaaa ttaattataa aaaaactcta ccttactaac tatacagctc
      121 acaatctatc tatttatgaa aaaatatgat ataattgcat tcaaaaagga aatctttcaa
      181 tatactagaa tttaagcatt aactaatttt atctaatatt attactaaac taaaaaatat
      241 ttgtatttgt agcattggag caaataactt aatgtatatt cattttaaat acttatttaa
      301 aatttagcat ttaaattatt tacaaattaa catttaaata cttctagcat aaatacagtt
      361 gtcatcaaaa tttaaagtat cttatggagg aataccatgc aaaatcataa ttatataaaa
      421 atgttaaata aaaaggtaga agtattatct aaggaattag atagtagaaa ttttaaatta
      481 aaaaccttaa atgaaataga ttttttttct aaaataccaa acagagaatt tatgtaccaa
      541 catattgatg actgtatttc gaaaaatata catataggaa tcatgttatt aaatatcgat
      601 aactttaaat tatttaatga tatatatgga tacctagtcg gagaaaattt attaattact
      661 ctaactcaaa acctattgca ctttattcca gaatcaaagg gaatagtcgc ttatttaaaa
      721 ggagaaggtt ttttaattgt acttccaaat gtatcaaata aagagttaaa taatatgggt
      781 gcaactatta tagagagcgc ccgagaatta aaaataagac ttcctaatga aaaagaaata
      841 tactccaatg ttacatttag tatcggcagc actatctgga atggtgaacc aaacatgact
      901 actttgatgc ttttcaacca agtgtatagt gctttaaaaa aggctaaaag tgaaggtaaa
      961 aatagataca ttctatttga ttttaaagat aatagttttt acaataaatt agaaaatgat
     1021 aaggagttct tatcagaaat atcacatgtt ctacaaacta aacaaattga agtattttac
     1081 caaccacttt ataatataaa aaataatact atagttggat gtgaggcact tgcaagatgg
     1141 agacacccta ttaaaggact tcttactcca gataaattta ttcctttatt tgaacagttt
     1201 ggtctgataa cggaattaga tttgcattta tttgaagaga actgtaaaaa tataagaaga
     1261 tggattgata acaatgatgt ttttgttcca atttgttgta acttttctcg tcttcatttt
     1321 tttaacccta attttccaag tatgctaaaa ggtattactg aaaagtacag cgtctctaca
     1381 tcaaatttta gcattgaaat aacagaaaat atattaatag aaaatacaaa tacaattatt
     1441 caacaactag ctgaacttcg ttcttatggt ttttctgtat caatggacga ttttggttct
     1501 ggatattcat cttttggtat gttgcaaaat ctaccttttg atataatcaa aattgatagg
     1561 atatttttat ctcataattt aaatgatttt aaaaatacaa ccattttgta ctctataata
     1621 aacattgcaa gagctcttgg gatgcttaca gtatgtgaag gtgttgaaac agaacaacat
     1681 gtagaattta ttaaaagtat aaattgtgat atcgcccaag gttattatta tgcaaaaccc
     1741 atggagtgca aagaatttga aaaattacta tctaaagaga ataattcaga gaaaatgttt
     1801 ataaaatatt ctgatatagg aaaatcttta atagagaaga tatttgatgg attttttatt
     1861 atgcaagata tgaaaaaact aagagctaat gtcactgaag acattcaatg gtttgatttt
     1921 tacaacaaaa ctgaaattac atcttttcct aaagtaatag agcattttga aaaacatatt
     1981 aaaggtaaaa aattttctat tctttacaaa agtataaccc cacataatac tgaatcagat
     2041 tcattattaa ttagtggaga agccgtttta tcagatgaag cttctgaaca tcaaaaatat
     2101 agcaatttct actttagtgc aaattgtata atttcagaaa ataaattact cttagcaaaa
     2161 ttacatttgg atttaattaa aactgctggc tatataaatg aatttgaatc atattcaata
     2221 cctaaaatga caaatacaca tcttgatgaa agtaatattt tatcacaatt ttattcaatg
     2281 ttgcctgtag gtataattcg ctacgattta tttggtgata tgattattac ttatatgaat
     2341 gaagaaatgt ttgatattat aggttataca aaagaacagt tttttggtga gatgggcgga
     2401 aatttaagag caattgttca tccagatgat ttagactttg tttataaaaa aagtttggac
     2461 atgattgaca caaataaaat tgaaccattt ttatatagat tcataaaacg agatggtcaa
     2521 attgtcacag ttatgtatac acaatgtaac ctttctgcaa ttgatggaag gtcgattgcc
     2581 cagggtatgt atgtagatgt aaataaaata aaaaatttaa caaaaatcta gtacaaagta
     2641 ttttattttg gagaaattaa tatgtttaaa gaaatttttt taagaacttt tccaggattt
     2701 ctaattataa gagattctaa ctacagaatt atatttataa atgataattt aaaaaattta
     2761 attaaaagtt atatgcaaga taatccactt ggaatgacta atatagaaat agctaagaaa
     2821 ctccctgata atattgctaa attttttacg gattctcaca atattcaatt agattgggaa
     2881 aaaaattatc cttatgataa aatttcaaat tggattttag agtttaaaaa agatactaca
     2941 agctattgga atgttttaga atacaaagta gatgttgatg aaaaaaccta cataattact
     3001 atggccaatg atataacaaa actctatgaa gaaaataaga gaaatcttca ctactcaatt
     3061 actgacccat taactggagc atataataga aaatatttaa atgatagatt tgacatattt
     3121 ataggagatt atattgtact aattgactta gataatttca aaatgataaa tgactatgaa
     3181 ggtcataatg taggagacaa gattttatgt gattttgttt cacttttaaa taaagaatta
     3241 ataaattcta cttctattat acgattagga ggagatgaat ttattgttat attctctagt
     3301 gatgttgaca aaaattatgt ttattcacag ctagaagcct taagagaaaa ttttttaaaa
     3361 gtattctcta agtataagta tctttcattt agttatggtg ttgatacagt aaaaagaaat
     3421 ttaaaattga caatagttga attggataaa aaaatgtata aaaacaagga gaaaaacaag
     3481 aaaaaatttg ataaaaatga ttattaacat tgttatttta aaataaatta aaattatctt
     3541 tcttttttta aacttgtaca ttagtttata aatatgtatc tatacaagat attgacatta
     3601 agtactgaag catatattgt ttcagttctt ttttgttaaa attaacttat ttaacaaaga
     3661 tatcatagtt taacaaagct tttaatttaa atatattgat gtataatata attaaaatat
     3721 aatagcaaaa agaaaggagg aatatcatga gcatactcct aaaaaaagca cccaaattag
     3781 caaaacatat tataacaagt ttttatataa atcgagatat caatgaagta ttaaaatacc
     3841 tttgtgaaaa tgttacatgg attggaccag gagaacagga atttctcact tcttttaatg
     3901 aaataaaaaa ttatttttat gcaggtcaag atgaaattcc ttcttgtgat ataaataatg
     3961 atatatttga aactgtaagt gaagatgaga atagatgtat ggtattagga agatatactg
     4021 ttcgaactaa agaaaatgct caaatgattt tagaggtaaa tcaacgttgt acatttgaga
     4081 taattgagga tagaggaaaa ttattaataa agcacatgca catatcaaac ccttatggtg
     4141 aaatgcaact tgatgaatac tttcccacta aaattggaac tcaaagttat gattatttgc
     4201 aaagattact aaaagaaaaa acagaagtta ttgaaatgat tacaaataat atcaatggtg
     4261 gcttaaaagg tagcaatgat gatagtacat actcattttt ctatgtcaat gagggattgc
     4321 ccaaaatatt aggatacaca tatgatgagt tcatggaaat gagtggtggt tcagcagtgg
     4381 gtgctgtata tccaccagac ttacctaaag cactagaaga ttgtgaacga tgttttgcaa
     4441 aaggacctac atattcatct gaatatagaa tccgaaaaaa agatggaaca ttaatgtggg
     4501 tattagattc tggaatgaaa tcgttaaata gtgatggcat agtaaaaatt aatagtatta
     4561 ttactgacat aacccaacta aaaaacatag aatctgaact aaaattagaa agagagcgat
     4621 atcgtattgc tttgcaaaat attacagata tcatgtttga gtatgatatt gaaaacgata
     4681 cccttataaa atatcaacgt gtagaaattg acaaaaaaat tgaattagaa aattttgaaa
     4741 caaaaaatta ctccaaacta ttagaatctg gtaaaattat tcaccttgat gatattggaa
     4801 aactactaga agtacttcat ggaaatcttc gtgaaacaat tgagataaga caagtaaatt
     4861 cattaactaa aaatgaatgg agatggattc gtgttcaatg ttcagttata tatgatagtg
     4921 accataaccc tatcaaaaca ataggcgttt taaaagacat aactgaagat aaatctaaat
     4981 tagaaagttt aattaatcag gctcaacgtg acccactaac acaattatat aatcaaagag
     5041 tatcacaaaa tcttattgaa gagtatctat gtagttctga ttccaaaaat aatgatgctc
     5101 tattagttat tgatattgat gattttaaaa ctgtaaatga tacttttggg catctagagg
     5161 gaaatgaagt tttagttgct gtatcaaaaa tactattaca taacacacat gataaagaca
     5221 tagttgctcg aattggtgga gatgaattta caatatttat aaaatcatta acaaaggatt
     5281 taataataaa aattactaat gatattttaa atgatgcgag taaaataaaa gtaaaggaca
     5341 atcataaaat aacattaagt attggtattg ccttcactga tgattcaact aaattatata
     5401 aagatttatt ttctaaagct gataaagctc tttatctatc aaaagcagat ggcaaaaatt
     5461 gttatagtac atacgaataa taaagctatt tatacgacca agaatgaacc tttaaattta
     5521 atttcatagt ctaacaatca aagagttgaa gctaatctta tagctagtca actagttcct
     5581 agaaaaaatt taaacaatat atttattgat actgctcatt tttctaaaga gcaaatttat
     5641 ataaaaaatt aaacttttat atacatacag atactaatga taaacagtta tcattagtat
     5701 cttctatctt aaaacaaaat gataatttat ctatataaaa aagtgctttg ttgacagagt
     5761 tcactttttt atataggtac agaatttaag tataaataca tgttttattc tatacatttt
     5821 agttaatgta aaatttcttg attgtactct ctaacttttc aacattatac tatttatata
     5881 ccacttagtt aatataaaac tataaaagtg ttgcaattac tgggttttat aaagttaatt
     5941 tacataccac ttagttaata taaaacttta gcaccttatt tatctataga gctacagaaa
     6001 gtatttacat accacttagt taatataaaa catgtattta tttgttcttg tactattgtt
     6061 ttactctatt tacataccac ttagttaata taaaacaata tcaaaacaaa taaaaatttt
     6121 taataaatca ttatttacat accacttagc taatataaaa cattctggta ctgttgctat
     6181 ttatgttccg tattcagaat ttacatacca cttagttaat ataaaacttt ggagttgtta
     6241 cagattattt gccttatctc tctatttaca taccacttag ttaatataaa acccccaaat
     6301 aaactgggca ttttcaatac ttacacatat gcaactctct taaatttgca gtgaaccatg
     6361 agtagtgcaa atgataaagc ttatcatata ccctcaacac cttatatttc aagcgttaag
     6421 ctctactttg taacaaatat cgctcactgc aaaactattt acatttttat tatatcataa
     6481 atatattatt tttgaatatc tataccaatt tgtggtataa taaaagcaag aagaactaca
     6541 atctatttag acgtagagtg aagttcaaat actaatgttt attctttttg aacttgattt
     6601 ttagttcaaa actaaagtca ctcctgcctg tgtgaactat acccgctgtt ttatacagtt
     6661 aaaacatgta aaaatagtta taaagtacta gtgtaactca ttagtactct ttttattata
     6721 ttcatataat taatatctat attttaaagc ttactatcta aaaaagagca cgtcaaataa
     6781 aaccctagat atttaatttt tttagagata cttaaaataa attatgctct ctctgtactt
     6841 aaatatttcc taaattcaaa tggagacata gaatttaatc tattttgata tctaaaatta
     6901 ttatagtagt atatataatc atctattgct ttaactaatt attcatatgt atcaaatttc
     6961 cttaaatagt acatttctac ctttaaaatt ccccaaaatc cttccatagg agcattgtca
     7021 aggcaacgac caactcttgt ataatatatg tatatctaac accattaaga aatatatatt
     7081 ttataatata tatttcttca tttaatgcac ttagaaaagc aaagaaaata cagattagta
     7141 cactttttag agaaaaataa aattaataca tctaaaatat taaaaaatac tttagataat
     7201 ctaaagacac atctcaaagt ttttaactaa attataaaac taattacaaa tgctatgtaa
     7261 cgataataaa taaaaaattt acttcgctta agcgacgtgt cgttgaaata tttcatgttg
     7321 ccaactgatt cataagtcga taagaaaaga acactaatta tttagagttc ttttcttagc
     7381 tacaagtgga ttataatact tttaaatctg tgagaaaatc atatataata atgatatgaa
     7441 caagtaaaga aaatacaaaa atgacaaaat tataaataaa atattataaa atcataaggt
     7501 cataattgtt atattgtaga aacaaagtaa agagagaggg tggagaataa atgaatcaaa
     7561 aaaaaataaa gattagtatt aaaatggtaa ttataggtat attttctgta attattgctt
     7621 ttgtattatc cagattattc gttgagtata ttgatgaatt attaaggtct aattggcaat
     7681 atcagctatt tgaaagtgag agttcatata taatatttgc caattgtatt atgttctcga
     7741 atatattatt agaaatattt ttaatatata tttgtagaaa atttagaaaa atctaatatg
     7801 gtcaacgtat ttcgaatcga atacttcatg attatttagt ctattgtgct agttaaagtt
     7861 taattcccat ataaagtaaa ttgattttag taaaacaaat cacccctaag ggttagttta
     7921 tggataagtc atccaaatat taactcttta atttttgcta tagattcatc tttgctcata
     7981 a
//
LOCUS       DAYATN010000157         7954 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.158, whole genome shotgun sequence.
ACCESSION   DAYATN010000157 DAYATN010000000
VERSION     DAYATN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.158"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            26..466
                     /locus_tag="ACGI8A_003265"
     CDS             26..466
                     /locus_tag="ACGI8A_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0181244.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKN
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            793..1020
                     /locus_tag="ACGI8A_003266"
     CDS             793..1020
                     /locus_tag="ACGI8A_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFH0181245.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            1046..3166
                     /gene="feoB"
                     /locus_tag="ACGI8A_003267"
     CDS             1046..3166
                     /gene="feoB"
                     /locus_tag="ACGI8A_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFH0181246.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKNSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(3299..4117)
                     /locus_tag="ACGI8A_003268"
     CDS             complement(3299..4117)
                     /locus_tag="ACGI8A_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0181247.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            4336..4608
                     /locus_tag="ACGI8A_003269"
     CDS             4336..4608
                     /locus_tag="ACGI8A_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181248.1"
                     /translation="MKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKAVKLE
                     IFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     gene            complement(5661..7754)
                     /gene="pdcA"
                     /locus_tag="ACGI8A_003270"
     CDS             complement(5661..7754)
                     /gene="pdcA"
                     /locus_tag="ACGI8A_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdcA"
                     /protein_id="HFH0181249.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVKNPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SVQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         2813 a         1125 c          980 g         3036 t
ORIGIN      
        1 tttaagatgc taggaggtaa ttactatgtt taaaattaaa cactttaatg atttgagttt
       61 agatgaattt tatgaaatag caaaatcaag atatgaggta tttgcttgtg aacagaagat
      121 attttcatta aacgattatg atgatataga taaaaactct tatcacatat ttttaaaaga
      181 aaatggattg atttgtgctt acgctagaat aattcctaaa gaatacagct cttataatga
      241 tgtttccatt ggtagagttt tagttctttc tagtcataga agaaaaggat tagcaaaaca
      301 aatgatggat tgtgctatag attttataaa agttaactta cacgaaaata atataacctt
      361 atcagcacaa acttatataa agaaccttta cttatcttgt ggttttaaag aaatctcaga
      421 agtatacgac gaagcaggta ttgaacatat taaaatgaga ttatagtgtt gacaagctaa
      481 tattgttaat atataattta agtataaatt aaatattgaa ttgatattta ggtatttgaa
      541 aataccttac atagagtcag aaggcttttg ctttctgact tttttattat caaaattcaa
      601 taattatatg gattcaaagt gttaattttt attctatatt ctactttccc caaaagtaag
      661 aattataccc aactttgtat gataggcatc ttgcctatct tttttttgta aaagtaacaa
      721 ggtaataata taatgcatag tttaaaataa gaagattagc ccaggctaaa tttcaagcat
      781 ggaggtgcta ttatgaaaaa ccttaacgat attcaagtga aaaaaactgt caaagttgaa
      841 gatatattat ctagtggtaa tttaagagaa agaatgttag cgcttggtct tactagagga
      901 gctgttatag atgtagttag aaaaggtcca aaaaataatt taactgtcta taaaatcaga
      961 ggtagcaaga tagctctacg acaagaagaa agtagcttaa tattagtttc tgacatttaa
     1021 aaatttctag caggaggata ttaatatggg gttgacacat aactcaacta aaatgagttc
     1081 tttaaaagac atgtttgata tagacaataa ggaagaccag tttgtaattg cattggctgg
     1141 caatccaaat acaggtaaaa gtactgtatt taatcatctt acaggactta gacagcatac
     1201 tggaaactgg ccaggaaaaa ctgtagctac agctcgtggt aattttaaat ataaaaatac
     1261 cgaatatgca ttaatagatt taccaggaac atattcttta tttgccttat cacaagaaga
     1321 aattgtagct cgtgatttta tatgttttgg taatccagat gcagtaatag tagtatgtga
     1381 tgctacatgt cttgaaagaa atttaaattt agtatttcaa gtgatggaac ttacagataa
     1441 agttattttg tgtattaatt taatagatga agctagaaaa aaaggcatta ctattgataa
     1501 aaaactttta gaggattctc ttggtattcc agttatttta actgctgcaa gaaatggctc
     1561 tggtatggat gaactattgg atactttaaa tgatgtatct tttgacaaat ataaattaaa
     1621 taataaacct gttagataca atgaaaatat agagaatgtg gtaaaatcaa ttcaaccaga
     1681 attggataat attataccag gtataaattc tagatggttg ggacttagac ttattgatgg
     1741 cgatgaaagt atatttgaat ctatgagtaa ttatatagac aaaaattcta tagatgctat
     1801 taatgaagta aagaaaaaga ttccagacaa tataaataaa cagaaaataa gagatgagtt
     1861 tactaaaatc aactatgatt atgctaaaaa actgagtgat gagtgttgtt ctaatgttgc
     1921 taaaaaatca actgatagag aagaaaaagt agataaaata ttaacatcta aaatatttgg
     1981 gcttccaata atgctcttat tattaggtac tatattgtgg attactatag aaggagctaa
     2041 ctatccatcc actttattat ctaatttatt actcggattt gagccaagta tttcgggtat
     2101 attaaatagt attaattgtc catcatggtt aaatgatatg ttggttttag gattatacag
     2161 aacattagca tgggtaatct ctgtaatgct acctcctatg gcaatatttt ttccattatt
     2221 taccctactt gaggattttg gatatttgcc aagagttgca tttaacttag accacctatt
     2281 taaaaaagct tgtgcacatg gaaaacagtg tttgactatg tgtatgggat ttggatgtaa
     2341 tgcagctgga gttattggtt gtaggataat tgactctcca agggaaagac ttatagctat
     2401 acttacaaac aactttgttc cttgtaatgg tcgttttcct actttgatag ctatatctac
     2461 aatatttttt agctcagtta taaccaactc tttcgtatca agcgtagcaa ctgcactttg
     2521 cataacttta ctgataattc taggtgttat aataacattg ttggtttctt acactctttc
     2581 taagacgcta ttaaaaggag ttccgtctac atttacatta gaacttcctc cttatagagt
     2641 tcctcaaata ggaagaactt tatatacctc tataatagat agaacaatat ttgttttagg
     2701 aagggctgta atggtagcca tacctgctgg tgtcataact tggatatttg caaatatata
     2761 tataggagat ttaagtatat tatcacatgt tgcaaacttt ttagaccctt tagctaaatt
     2821 aattggtctt gatggtttta tattacttgc ttttattctt ggattcccag caaatgagat
     2881 agttgtacct atacttttaa tggcttacct agcaactggt tctatgatag aattagatag
     2941 ctttagtgct ttaggtcaag ttcttagaga acatggatgg acttatttga cagctttaaa
     3001 tgttatgctg ttcagtttac tgcattggcc ttgtgcaaca acacttttaa caataaaaaa
     3061 agaaactggt agtttaaaat ggactgcctt aggattctta atgcctacta tacttgcttt
     3121 tgttgtttgt tttttaacaa ctacagttta caatttattt atataattag tattaactga
     3181 cagtagacat atattagaga aaccatttaa aaatttatat actttattat aaagcataga
     3241 gatatattca atgataagta aaattactta acatttgaat atatctctat tttaattctt
     3301 atctggatac ttggattgaa atttcagtca tataattatc ttcattactt tcacaaaaaa
     3361 agtcaagtat actctcctca tacgaatttc caacaatttt atattcattt tcatatatat
     3421 attttttcaa tttttcgtaa gatttatatg aatcctcata ttttccaata tgttttatgc
     3481 aagcatacat tccttctggc tttacatata gctttttatc ctttattttt ttatctatct
     3541 tcaaataaaa acttgatata cttaaatagt ccccattttt tatattttca ttacttacta
     3601 ttactcccaa ctcatatcct tgatacaaat ttttcgattt acaataattg gtaaaatcta
     3661 ttaattccat cataaaatca ctattactca tattactcaa atcgcttgat agaactaaat
     3721 attcttcaaa ctttttggat atataaattt cttcactatt cattttagaa attccttcaa
     3781 ctgttatatt tattctattt tgcatcttag aacttatctt tcttagtttt tttatttttt
     3841 ctcttatctt tttttcttct tctttaagca ttttcacaca aagctgggga gttctatttt
     3901 gtatatactc ttttatttcc tctaaactca ttcctatttc tcttaatgat tgtaatatat
     3961 caagtgtatc atattgataa actgagtaat atctataatt atttttatct ttatattcag
     4021 gagaaaaaat acctattttg tcatagaaaa ttaatgtctg cttagatata ccacacaatt
     4081 tagcaaattc acctgttgta aaatactgtt tttccaaggg ttatacactc cttttctttt
     4141 tgttgtctat atagttacta tattctttat tatttactaa atcaagtagt tattatttta
     4201 gaaaatcaat atggttttga ttttaaagct taaatatcag ttagatgtca gttataaata
     4261 ttttaccctt tcccatcata aaaaagatat cccctcttaa cttttggatg gaatattata
     4321 tttatacttg ttagagtgaa aattgtaata ggtgctacaa taagatattt aattaaacat
     4381 aaagttaatt attgtaataa aaaatcttta gataaattag agttaaagta cggaaatata
     4441 gatagagaaa aagctgttaa actagaaata ttttatcagt atttaataga acttgaatat
     4501 atagaaatga gtttattaat aagaagatta gatactgcaa taacatcatt agcattagta
     4561 tctattataa caatagcatc atattattta atcagaaaaa atgtgtaatt atgtaagcta
     4621 tttataataa aacactgata aaattaataa taaaaaggta ataggatttc tatactacct
     4681 ttttgttttt ataatatagt gtaattttat tatatttttg tatgctaatg taactagata
     4741 atcataagat aacacattaa aagaaaatta tgaaaactta cattctgtat agttaatata
     4801 aaacataagt aatataaatt tatgtaaagc aacaaaagat agatttacat tccatatagt
     4861 taatataaaa ccttagagaa agcgaaaacg taccattaaa atcaaatatt tacattccat
     4921 atagttaata taaaacgttg taatcatagt tccaattagc cactttaaca tgcatttaca
     4981 ttccatatag ttaatataaa acaccaaaat aaacttagta ttttcaacac ttacacgtat
     5041 acaactctcc taaatttgca gtgaacgagc aatagtgcaa ttgataacgc ttatcatgca
     5101 ctctcaacgc cttatatttc aactgttacg ccaaattttt tcacaaatat cgctcactgc
     5161 aaaattccta taattttatt atatcataaa tatattactt ttgaatattg ataccaattt
     5221 ataccataat aaaagcaaaa aaacgtaatc tatctacatt agatttaaat tctataactt
     5281 aaagtttttt acataatatt tatgcttgtt taatcaatct aatatatcat tcttgtatat
     5341 tgcttttaat agtctttttt cttaaataaa tatctatctg aacaagcata gtttaattaa
     5401 aaacttttgt attcccttaa aactgtatct acctcttatt attttattta tgatatatta
     5461 tttttaatct aaaaatagat atctattttt attatggtta tcccttttta aaatacttta
     5521 atcatatact attcccaatt taacatccat tgaaatgcta ttttcttata ttgcaattta
     5581 taattgatat atcttcaata taataattta atgtattttt gattttaaag cttttcagtg
     5641 atattattga taaattagaa ttaattatct agctttaaaa ggtcaaagat ttctgttgct
     5701 gtctgcggtt ttccaaataa atatccttgt atgtaatctg gctttatttg tttaactaaa
     5761 tcatactctt cttgtgtttc tacaccttct aaacatactt ttattcctac atcatgacaa
     5821 attgcaacaa taaaatgtat aaatgtagca tcaaacttac tctttaatat atctttaaca
     5881 aatactctat caattttcac tatatctatt ggagaaaatt ttaaaacttc tagagacgag
     5941 tatccagtac caaaatcatc catagcaata taaattccta aatcttgtaa agccttaaat
     6001 ttaaactgta aaatactcat attttgtact gtatgacttt ctgtcaattc taaaacaaga
     6061 ttttgataag gaaaattttc atcatctata atttttacta tatcttcaat aaatgtgtct
     6121 tctaatattt gaacagctga aacgtttaca ctcactttaa aaaaaggata gtaatcaatc
     6181 catttcctaa aagtacgtaa tgccattcga agcacccaag cccctacaat atttatcata
     6241 tcattttctt ctaatattgg aataaattcc aaaggagaaa ttgctttgca tttgtcatta
     6301 gtccacctta aaagaacttc tacacctatt attttatgac tttctgtatc tacttgtggc
     6361 tggaatcgaa gactaaaccc cctaaaatca tcattaattg atgcctttaa atcccgcatc
     6421 atttctaatg aatacatttt attttttaat atttcttggg agaaaaaaac aagcctgttt
     6481 ttaccatgct ctttcgcata ttgtaaagag taactagcac acttataaag ttcttttact
     6541 gtatctccat gttttggata tattacacaa ccagcagaaa ttgttatatt taatccatat
     6601 tttctccata gttgaaggtg tataatcata ttctgtattt gattatatag agttagaatt
     6661 tcattttctt caacattatc aacaagaatg cccatttcgt ccccatcaag tttataaagt
     6721 tcagcattat ctggtaaaat agattgtaca gactgtgcta gtgtttttat tatattatct
     6781 ccaaaatccc tatcatacat ctcattaaca tttttaaaat cgtcaatatc taataatact
     6841 atgcataatt tttcaatcat aggatttgaa attttatctt caaatacact cataaattca
     6901 tgataattca gtaactgtgt caatgaatca atcttatttt gtttacctaa ttgcgtcatt
     6961 attcctgcaa aaatacttgg ctctccaaat tcatcacgca ttagttgacc tctacatctt
     7021 aaccatatat attctccact acgattttta gccctaaatt ccactgtatg ataatccatc
     7081 tggtttttac ctatttcagt gtttgactta taaaaacgat tccagtcttc tggatgtaca
     7141 atttttttcc agtaaacaag cggattttta acaatttctc ctggtaagtc aaataattca
     7201 acttgtgatg gtgaatatcg aaatacgcct gttttcatat tgcaaatgta tatatattca
     7261 tctgtacctc taatcaaagc atcgtaaagc aatgtagaat tataatcaaa attgtctctt
     7321 gaaaaattaa aaactccttt ttcttcttta ttatttgata tttcatgaaa ttcattaatc
     7381 ttaattggtt tatttaagct tgttgagttt gcaatatcta ttgattgatg catatgaaca
     7441 actgtgccat cttgccatgt aattaaacta tcataatttt caaaaacacg atttaactta
     7501 ttacattttt cataccactt tattaatact ccttttttat cattctttaa taattctaaa
     7561 actttacaaa aactacaagt agaatttttt tcaggatata atacttgcca acaaactttt
     7621 ccttctggat ctaaaatatt atattcttct ttcatttttt tgttcataaa aataatttca
     7681 ttagtatgaa tatttgtaac atatatatta atctgtagtt gatttaatat aacttcaaaa
     7741 ttatgtttgt tcatatactt atctcctcca tagttactgc tctttataac aacattgtta
     7801 aaattattct ttttagaaga tttacttata acttatacat atacactaaa taccttaatt
     7861 taattagaaa gtttattact tcatatttta aattataatt gataaaaaaa aatacagcaa
     7921 tatagtatta ccaatttaaa aaaaattaat atta
//
LOCUS       DAYATN010000158         7801 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.159, whole genome shotgun sequence.
ACCESSION   DAYATN010000158 DAYATN010000000
VERSION     DAYATN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.159"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            27..101
                     /locus_tag="ACGI8A_003271"
     tRNA            27..101
                     /locus_tag="ACGI8A_003271"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="ACGI8A_003272"
     tRNA            116..200
                     /locus_tag="ACGI8A_003272"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="ACGI8A_003273"
     tRNA            210..293
                     /locus_tag="ACGI8A_003273"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="ACGI8A_003274"
     tRNA            317..391
                     /locus_tag="ACGI8A_003274"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="ACGI8A_003275"
     tRNA            416..492
                     /locus_tag="ACGI8A_003275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="ACGI8A_003276"
     tRNA            502..577
                     /locus_tag="ACGI8A_003276"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="ACGI8A_003277"
     tRNA            586..661
                     /locus_tag="ACGI8A_003277"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="ACGI8A_003278"
     tRNA            664..752
                     /locus_tag="ACGI8A_003278"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="ACGI8A_003279"
     tRNA            756..831
                     /locus_tag="ACGI8A_003279"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="ACGI8A_003280"
     tRNA            838..911
                     /locus_tag="ACGI8A_003280"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            1011..1086
                     /locus_tag="ACGI8A_003281"
     tRNA            1011..1086
                     /locus_tag="ACGI8A_003281"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1044..1046,aa:Lys,seq:ttt)
     gene            1094..1167
                     /locus_tag="ACGI8A_003282"
     tRNA            1094..1167
                     /locus_tag="ACGI8A_003282"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1126..1128,aa:Cys,seq:gca)
     gene            1175..1251
                     /locus_tag="ACGI8A_003283"
     tRNA            1175..1251
                     /locus_tag="ACGI8A_003283"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1209..1211,aa:Arg,seq:acg)
     gene            1264..1339
                     /locus_tag="ACGI8A_003284"
     tRNA            1264..1339
                     /locus_tag="ACGI8A_003284"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Val,seq:tac)
     gene            1415..1828
                     /locus_tag="ACGI8A_003285"
     CDS             1415..1828
                     /locus_tag="ACGI8A_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181250.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2137..5568
                     /locus_tag="ACGI8A_003286"
     CDS             2137..5568
                     /locus_tag="ACGI8A_003286"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFH0181251.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTRESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5810..7738
                     /locus_tag="ACGI8A_003287"
     CDS             5810..7738
                     /locus_tag="ACGI8A_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFH0181252.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3073 a          989 c         1559 g         2180 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actgaaattt aatgtgggcc attagctcag ttggttagag cgccagattg
      961 tggctctgga ggtcgtgagt tcgactctca ttatccaccc caacttatat ggtctattag
     1021 ctcagtcggt agagcacctg acttttaatc agggtgtccc gcgttcgagt cgcggataga
     1081 tcaccaaaaa ggcggcgaca tagccaagtg gtaaggcagt ggactgcaac tccttgatcc
     1141 ccagttcgaa tctgggtgtc gcctccaata aaatgcgctc atagctcaac tggatagagt
     1201 gtctgactac gaatcagaag gttaggggtt cgagtccctt tgggcgcacc atatgaatag
     1261 cgagggatta tagctcagct gggagagcac ctgccttaca agcagggggt cacaggttcg
     1321 agccctgtta atcccaccag tgcaaacctc tagtaaaact actagaggtt ttatttttga
     1381 ttgaaaaagt ttttatattt aggaggtaaa aaatttgtat atagtaatag gaaatgaaat
     1441 agttgatagt gaagaactta aaataattat agataaaaat agtgaattta aagttgaaaa
     1501 agatttaagt aaaagcacaa aaagagaaga tgtaatagca tatcagttaa gtatagattt
     1561 aaattaccta gattctctta taaatgagca atgtaattta gccagtctat ctgatgaaga
     1621 gaaatttgat gaatatatga ctttgtctga tgaacttgca ctggatttag aagagttaat
     1681 gccaaaatat acaataataa atgctagagc atataaatta gacgaggtag atggaattgt
     1741 aaaaataata ctagctgtag cgtatgcaga tttagggcac ttgaaacttt ctgatgtagt
     1801 aaagaggcta tctagacaag tagattagga tatgaatatg aactatatta aaaaaatgta
     1861 taaaatctta cgtaaagatg aaaataatta tgtaaacata gaaaaaaaca gtataaaatc
     1921 aagtcaattg tagaaatttt cacataaatt catctgaaat tctataaaaa atgccttaat
     1981 tttaatgagg taaatcgagt ttacacagtc taaaaattac ttatatttat aaatttatct
     2041 tattaattaa ggaactttct taaacatctt caaaaataaa gtattttagt ctataatctt
     2101 taatattaca caaacaataa aggagaagaa tagagtatgc ttaaaaagtt taataaaata
     2161 ctagtggcta atagaggcga aatagctata agaatattta gagcctgttc agaattgggg
     2221 ataaaaagtg ttggtatata cagtaaggaa gataagtatg gattgtttag aacaaaagcg
     2281 gatgaatcat atttgatagg agaaggtaaa gggccaatag atgcatattt agatatggat
     2341 ggaataatag acctagcaaa gagaaaaaaa gttgatgcta tacatccagg atatggtttt
     2401 ctagcggaga atgcagaatt tgctcgtaaa tgtgaagaga atggaattac ttttatagga
     2461 ccatcatcta aggtaatgaa tatgatgggg gataagatta actctaaaaa aatcgcaaaa
     2521 gaagttaatg ttcaaactat accaggagta gaaaaagcaa taagaagtac tgaagaagca
     2581 aaggaagttg caaacaaaat aggatatcct gttatgataa aagcatctaa tggcggtggc
     2641 ggtagaggta tgagaattgt acatcgcgaa gaagacttag agcttgaata tgaaactgct
     2701 tgtagtgaat caagaaaagc ttttggagaa gatattatat ttatagaaaa atatatagct
     2761 gatccaaaac atatagaagt tcaaatatta ggtgataatt atggaaatat agttcactta
     2821 tatgaaagag attgttcagt tcaaagaaga catcaaaaaa taatagaata tgcaccagct
     2881 ttttcattag atgataaagt aagaaaagag atatgtgaag atgcagttaa gttatcaaaa
     2941 catgtgggtt attctaatgc gggaacatta gaatttttag tagatgcaaa tggtgggcat
     3001 tattttatag agatgaacac aagagtacag gttgaacata ctgtaacaga gatggttaca
     3061 ggaatagata tagttcaaag tcaaatacta atagcccaag gttacagctt agatagtgaa
     3121 gaaattaata taaaatcaca agatgatgtg gaaataagag gttactctat ccaatgtaga
     3181 ataactacag aagaccctaa gaataaattc atgcctgata caggtaaaat tcaagtttat
     3241 agaactggtt caggatttgg tataagacta gatggaggaa atggatttac aggggcaaac
     3301 ataagtcctc attatgatag tttattggtt aagacaatat cttgggatag aacattccaa
     3361 ggagcaataa ataaaactat aagatctata aaagaactta gagttagagg tgttaaaact
     3421 aatgtaggat tcctagttaa tgttttaaat aatcctatat tctcaaatgg taaatgtagt
     3481 actaagttta tagatgaaaa tccagattta tttgaaataa cagaaagtaa agacagagga
     3541 acaaaattac ttcaatttat aggagatgta attgtaaatg ataatgcttg taaagaaaaa
     3601 ccattatttg atgcattaca tgacccaaga atggataagg atggttcaaa atcagaagga
     3661 agtaaaatac ttttcgataa gttaggtaaa tctgcatata tagaaaagat aaaaaatgat
     3721 aagaaattac tattaacaga tacaacaatg agagatgcac atcaatcatt attagcaaca
     3781 agaataagaa catatgactt attaaaagct gctaagccaa cagaaaaata tcaaaaggat
     3841 ttattctcac ttgaaatgtg gggaggagct acttatgatg ttgcatatag atttttaaaa
     3901 gaatctcctt ggcgtagatt acaaaagcta agagaagaaa taccaagcat aatgttccaa
     3961 atgttgctta gagcatcaaa tggagttgga tataaaaact atccagataa tgtaatagaa
     4021 gaatttacaa gagaatctgc tagacaaggt atagatgtat ttagaatatt tgattcatta
     4081 aactgggttg aaaatatgaa accatctatt aatacagctt tagaaactgg aaaaattgtt
     4141 gaagcaacta tgtgttatac aggtgatata ctagataaga ctaaaactaa atacaatcta
     4201 gaatattata ttaaaatggc acaagaatta gaaagcttgg gagcagatat aatagcaata
     4261 aaagatatgt caggtcttct taaaccatat tcagcttaca cattagttaa agagttaaag
     4321 aaaaatgtaa aagcaccaat acatttacac actcatgata caagtggaaa tggtgtagca
     4381 acttgtttaa tggcgtcaga agctggagta gacataattg atgctgcatt agaatctatg
     4441 gcaggactta caagtcagcc atcacttaat gctatagtag aggcacttaa gaatacagag
     4501 agagatacag gaatagattt atttggatat gatgagttag gaaaatatta taaggattta
     4561 agaaaagttt ataataaatt tgaaagtgat ttaactaatt cttgtgcaga aatatataat
     4621 tttgaaatac caggaggcca atatacaaac ttaaaacctc aagctgatag tttaggatta
     4681 gttaatagat ttgatgaagt taaagaaaaa tataaagaag ctaatgaagt tgtaggagat
     4741 ataataaaag taactccatc ttcaaaagtt gtaggagact tagctatctt tatgactaaa
     4801 aataaattag ataaagacaa tataatagaa gaaggtaaaa atttatcatt cccagattct
     4861 gttgtggatt actgtaaagg tatgattggt caaccagaag gtggaatacc aaaagattta
     4921 caagaggtag ttctaaaagg tgaagaagct ataacagtaa gaccaggttc attattacca
     4981 gcagaagatt ttgatgaaat agctaaatat ctaaatgaaa agtatgatat aaatgctaat
     5041 ataagaaatg ttataagtta tgctttatat ccaaaagtat atgaagatta tatcaaacat
     5101 ctacaacatt ataatgatat atctaaactt gaaagtgatg tattcttcta tggattaaat
     5161 aaaaatgaag aatgtgaagt agaaatagaa gaaggtaagg tattaacaat aagattagtt
     5221 gaaattggtg aagttaagga aaatggattt agaactatag gatttgagtt aaatggaatg
     5281 gttagagaag tagaaataaa agataagaat ttctctggaa aaattaataa tgttgaaaaa
     5341 gctgatatga atgaccctct tcaaattgga gcaagcatac caggaaaagt tataaaaata
     5401 atggttaaag aagaagatga agtaaaagca aatcaaccat taatcgttat agaggctatg
     5461 aagatggaaa ctataatagt tgctaagact gatggagtaa ttaagtcaat caaagtaaaa
     5521 gaagatgata tggttgaaga taaacaatta ttaatgataa tgaagtaatt taattaaaat
     5581 aaattaaaaa gcactctgaa atgtttaata aaaaatgttt tagagtgttt ttatttattt
     5641 taattaaata tttgttaaaa aaaagaggac aattacctcc agatgtagaa ataaagctag
     5701 taaggaaaat gttatctaaa aattctaaat atagtaaata ttttccataa tatttcttag
     5761 gaaataacag caaagataaa atacataaat ttaaaaggtg ggtgcttata tgaaggttta
     5821 tgatagtaaa atgttgagaa atgtagcggt attaggacat agcggatgtg gaaaaacaaa
     5881 tctaatcgaa actatagctt acacagcaaa cactaataag atacctaaat taacagacaa
     5941 ggtaaatatg acttatagca tgggattaat acctatagag tataatgatt ataagtttaa
     6001 cttattggat accccaggat attttgactt tagcggagac gttgtgtcat ctcttagagc
     6061 aagtgatgca gcaataatag taatagatgc aacagcgcct attcaagttg gtacagagaa
     6121 atcattagaa ctaactgaga gtattcctaa gataatgttt ataaataaaa tagataatga
     6181 aaaggcaaga tacaaagatg caatagcaat gttaagagaa aaatacaata ataagatagt
     6241 tcctatgata agtcctatat acaaagataa aaactttgta aagttacata atgtttttga
     6301 aaacatagat gatttagaag gtgaatttaa ggaacaagct atgagtgtta aagaagcatt
     6361 aatggaactt atagcagaaa ctgatgacca aatattagat aaatatttta atggagaaga
     6421 actaactaca gaagagatac aaaaaggtat tattataggt atacaaagag gtgatattat
     6481 acctgtaata tgtggttcaa ctataaataa tataggcaca aaggaaatat tagatacaat
     6541 atcaagttat ttagaaccta tctttacaga ggaaagcaaa ccatttaggg gattagtatt
     6601 taaaactatg gttgacccat ttgttggaaa gatgtcatat ataaaaatta ctgagggtgt
     6661 actttctaag gataaagatg tttttaatat aaataaaaat gtaaaagaaa aaatagctaa
     6721 tatatataca ttgagaaata gtgaattagt agaaatagaa aaagctaaag ctggtgatat
     6781 agtagttata actaaagtta attctctaaa aacaggagac actatatctg cagataaaga
     6841 tgcagaagct ttagaaaaga tagattttcc taaaccacaa atatattatg cagtaactcc
     6901 taagaataaa ggtgatgaag aaaaagtagc atctgtatta aataaactag tagaagagga
     6961 ccctacatta cattggtata gaaatacaga gacaaaacaa gcacttttag gaggtcaagg
     7021 agaacttcat ataaaaacaa ttaaaaataa aatgaaagat aagtttggtg ttgatgttga
     7081 attgaatgac cttaaagttc cttatagaga aactataaaa ggaacagctg atgtacaagg
     7141 aaaacataaa aagcaatcag gtggtcatgg tcaatatgga gacgtaaaaa taagatttga
     7201 aagatgtgaa tctgattttg aatttacaga agaaatattt ggtggttctg taccaaaaca
     7261 atatatacca gcagttgaaa aaggtttaaa agactctatg caaaaaggta tattagcagg
     7321 atatcctgtt acaaacataa aagctacgct ttatgatggt tcataccatg atgtagactc
     7381 ttctgaaatg gcctttaaaa tggcagcaag tgcagccttt aaaaaaggta tggaagaagc
     7441 acacccaata ctactagaac ctataatgaa acttaagata actgttccag aagaatatat
     7501 gggagatgtc atgggtgata taaataagag aagaggaaaa atatttggaa tggaaccaga
     7561 tgacaaaggg aaacaaataa tatttgcaga agctccacaa gcagagacat ttaaatatgc
     7621 aatagattta agagcaatga cacaaggtag aggatacttt gaaatggaat tagagagata
     7681 tggagaggtt ccatcacagt ttgcagaaaa aataattggg ttagctactg caaagtaatg
     7741 aacaaaataa atagaaaaat aattaatata tattattaaa aatattttta gggttttaac
     7801 t
//
LOCUS       DAYATN010000159         7771 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.160, whole genome shotgun sequence.
ACCESSION   DAYATN010000159 DAYATN010000000
VERSION     DAYATN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.160"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(256..1074)
                     /locus_tag="ACGI8A_003288"
     CDS             complement(256..1074)
                     /locus_tag="ACGI8A_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0181253.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            1242..1823
                     /locus_tag="ACGI8A_003289"
     CDS             1242..1823
                     /locus_tag="ACGI8A_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0181254.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            1820..2008
                     /locus_tag="ACGI8A_003290"
     CDS             1820..2008
                     /locus_tag="ACGI8A_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181255.1"
                     /translation="MKIITIGSSLITVLLFLSTMICGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYI"
     gene            complement(2178..3920)
                     /locus_tag="ACGI8A_003291"
     CDS             complement(2178..3920)
                     /locus_tag="ACGI8A_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloendopeptidase"
                     /protein_id="HFH0181256.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            4349..5725
                     /locus_tag="ACGI8A_003292"
     CDS             4349..5725
                     /locus_tag="ACGI8A_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HFH0181257.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            6036..6707
                     /locus_tag="ACGI8A_003293"
     CDS             6036..6707
                     /locus_tag="ACGI8A_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFH0181258.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            6896..7693
                     /locus_tag="ACGI8A_003294"
     CDS             6896..7693
                     /locus_tag="ACGI8A_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFH0181259.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         2860 a          952 c         1209 g         2750 t
ORIGIN      
        1 acatcacaag tgatttatct gacaaattaa gataatatcc tgattaaatt ctagtcattc
       61 aaacataaca tcacaagtga tttatctgac aaattaagat aatatcctaa ttaaattgta
      121 gatatttata cataacacta aaagtaattt gtttaacaaa ttaagataat atcctaatta
      181 aattgtaggt atttatacat aacactaaaa gtaatttgtc taacaaatta aaatattatc
      241 caattaatat aaaaattact tctttttaac tgggaagcat acctctgtta cccaattatc
      301 aggattacta tcgttgccag gacttacatg ataaatatta aacataggac catccacatc
      361 atagttatta tctgatatcc aattaccaat agcctcacaa gcgtctgcaa cttgattgaa
      421 atttccatta acaattgcag ttgcagcagt cacacttgga acagtcttaa atttaacatg
      481 ttcagtatct ttatactttc cagatacagc aacttgtact tcaacatcta cataatcttc
      541 tttatatcca gtatcataaa aaactgcttt agaatacttt gggggttcaa tttgaacatt
      601 ctggtctttt gtctctaaaa atgcttgatg ccataacatt ccttcgtctt gataagttgg
      661 tataaccctt ctaagtgtca tcatatattt tggtgcaaaa ttctttatag ttacatcata
      721 atttttcata atatcatttc ctccaattct tttaatagtg gtttctattt ttaatagttt
      781 tttctgtgta tcttctaatt gttcttttac ttgagaatgg tggatattta gatattttat
      841 gagactttct ttatcgttat attctgtcaa aatttctttt atagaataca agccaaagcc
      901 catatctttt agtgcatgga ttctgtttgt agtagaaagt tggtctgcac tatagtatct
      961 atatccatta gttttattcg tatgagaagg agttaaaagt cctatttcat cataatgacg
     1021 taacatacga atactaattt tacttagctt tgaaaaatcg cctatcttaa acatgttttt
     1081 gtctccttta taaaggtact tttagcttaa tattaatata aaccatatca taatgttaga
     1141 gtcaactaaa attataatta aattatattg acatatgatg aaaaaaatat tatactgaac
     1201 atgttcagaa ctgaaaatgt taattgtttt ggaggtctat tatgaaaagt aatgataaag
     1261 atgctagaga acgtataatt gaagttacat tgaatctttt aaatgaagta gatgatattg
     1321 aggagattac agttagaaaa attgctgagc gtgcaaatgt tggagttggt ttaataaatt
     1381 atcattttaa aacaaaggat aatttgttga gtactgccat aggagatgtg atgtcaaata
     1441 taatagcaga gctatatgac gatagtgtat atactctaag acctattgaa gatttaaaaa
     1501 atttattaaa aaaattgtgt gatactggat tacattatga aaaagtgctt ccttttgtat
     1561 tgaatcaatg tataaccaat ggagatatgc aagcagaact tgatattgtc cctatgcttc
     1621 gtaaaatttt tggaaataaa aaagatgaaa tgagtctaag aattattgcc ctgcaaatta
     1681 ttttaccaat acagatttct gctttatcta cagaatcatt tcagttatac agtggtataa
     1741 atataaagaa taaatatgag cgagataaat tcattgatat tttaatagaa aatattattg
     1801 gagaggatgt tgatgtaaga tgaaaattat aactattggt tcaagtctta tcactgtttt
     1861 actttttctt tcaactatga tttgtgggtt ctggattaag aataataagg taacagatgc
     1921 aagttctatt aagttccata tgaatagtgc tatttttaca gggatatttt tattgatttc
     1981 cacaatcctt ttaattatat atatataaaa ataataatat tattaataaa taaagtatca
     2041 gaaattataa agacaagagt cttaaaaatt tctgatactt taccatataa ttttattaaa
     2101 cattaattgt gtgtgaaaaa atatcactta tttcaatcta atactaaatc ttaagagtga
     2161 gtataaagta ttagaaatta agaagcattt tttagttcaa gcggattatc tataccaaaa
     2221 taatcatata aagtagagct tatttcctct aaatttccat cttggaaagg taattgtaaa
     2281 tttacctttt ctaccacatc tttaaatcca gcatcagtat tttgatttaa aaaatccata
     2341 tatacatcaa cagcttttct gtaatcttct tgtgataatt gccatatctg aagtgataca
     2401 tcagatgcaa gaccataact caagtaataa aaaggaactt ggaagttatg agatacaagt
     2461 atccaaaaag gagcattttt ttctctaagt atattattgg aaactccata ttcttcctct
     2521 aaatcaaaaa acaatttatt gatatcattt agactcatat atggattctt gtatataatt
     2581 tcttgaaatt catcataaag acaagcatca ataatagtat ttaaaacaat cgataagtgt
     2641 tcttttttta tagcttcaga ttgtttacca aaaaaatcat caaaatattt ataaaataga
     2701 atttctaaag atgttgaatg aacttcacat acatctatac ttggttgcat cttattgttt
     2761 aagttaatac tgttgtagta attataaaaa tgaccaaact catgagcaaa actagtaaca
     2821 cctaaaaaag aattatccca tgtattaaac aagaaaggtg ctttatattt atttatataa
     2881 gtagtatatc ctccaggtga tttgtttttt ccagaagaat aatcgtacaa gtcatatttt
     2941 ttcatatagt cgaagctctc ttttagtttt ggagatatat cttcaagtac tttatcaaac
     3001 ttcctaaatg agcgattttt atatacttta atataaatag aagaatcaga tggtttagat
     3061 gaaatctctc tatataatgg aactatgtaa tcttttacat cttgtctaaa ttttttagct
     3121 tcttcttgag aatagtcttt gtttaggttc atataggcat aatctatata attcttaaat
     3181 ccttgcttac ttgctatttc agttcttgtc tgtataagtt ctaagaatat gttaccaaat
     3241 ataggattgt atttttttaa gtaatctgaa taaattttca catattcttc agggctaaga
     3301 tttggtcttg acaaggcttc ttcaaagtct acctctatac catctattga tactgtagat
     3361 ttagctaata agtcttcata ttttacaact aggtcttgct cttttttaag caaaccttca
     3421 acttctttag aattaagttt tccaatgttc tttagataat taaatgtatc ctttccaagt
     3481 tctttttcta attctttaga gaatttagaa gttacaaatc tatcttgaaa gtctttgtat
     3541 gacatgtcaa catctactga tttactcata agatacttat attccttaga gtaaaattcg
     3601 tctgatgaat ctatgttatt tctaattttt gctatagtaa gtgtactgta aaaatcttga
     3661 taaattttat caacttcatt aaataaattt aattgttcct tagctgtttt agctgttaaa
     3721 agtttgtcgt tgcacttgtt aatgttttcg caaattgact gtatattagg acgcttatat
     3781 tccatttttt caaaaggaat agttcctcta acagaatctt tttttgtatc attagtataa
     3841 tttcttatgt atgatgcaaa tataataaaa actgcaatta aaattagaaa aacaaatatt
     3901 ttcttcttgg atttaaccat aaaaatcctc caatcatgga tatagttatt aattatttta
     3961 ctatattaaa tataaaaaat gcatgttaag aaaatttaac ataaagatat agataagatt
     4021 ataaataata taaaaaattt aacttcaaaa aagtatatga aatttatact ggtaaaatga
     4081 taaataaatt ttaagtacaa aatagtaaaa gctatgatag ttttagacct attatatgta
     4141 aaaaatatag atgtttttgg tgcaatttaa attaaaagat atatataaaa atttaaaata
     4201 ttccaaacag aaattataaa aattactatc attgtcttta tacaatattt atactataat
     4261 agaataataa tttagaggaa agctctaaaa ataaacaaat taagggtctt aacagtagtg
     4321 attttatgtt aaggaggtat aaagaaagtt gaaaacaata gttagaagac ttattctcta
     4381 cgcagatgga atgcgtccta ctcaaataat ggtatctggg tttgctgcga ttattgtaat
     4441 cggagcattg ttgttaacac tcccgatagc gtcgcagagt ggagagagta taggcttatt
     4501 aaatgctcta tttacggcta cttcagcggt ttgtgttact ggtctagtta tggtagatac
     4561 agcaacttat tggagtttat ttggacagat agttataata acattgatac aaattggtgg
     4621 tcttggattt atgaccgtag ccacaatgtt ttcacttatg gcacgaaaaa aaatacaact
     4681 aagagaaaga ttattaatac aagagtcatt aaatcaagct gatttatcgg gacttgtaag
     4741 acttacaaga tttgtattaa taataaccat aacaatagag ggaataggtg cactggtttt
     4801 atcaactgtc tttattccac aatttggttt gtctaaaggg atatggtata gtgtatttca
     4861 cgcaatatca gcgttttgta atgccggatt tgacttgatg ggcagtgtaa gtggcccatt
     4921 tacatcgtta aattcttatg taaacaactt tacagtatca atgactgttt gcgcacttat
     4981 agtgcttgga gggctaggat tccctgttgt acttgatatt gtaagaaaaa gaagattttc
     5041 aaaattaaat gtgcattcta aggttgtatt gttttcaact gctacactta tttttgtggg
     5101 tgcattattt atttttctta tagaatttaa tcaaaaagct actatggcgg acttgccttt
     5161 aaagggaaaa gtactatcag caatatttca atcagtaaca gcaagaactg ctggttttaa
     5221 tacgcttgat ttagctactt tacgagaaag tagtgtattt gtaatgataa ttttgatgtt
     5281 tattggagca tcacctgcat caactggtgg gggtataaaa acaacaacac tagctgtttt
     5341 aatcattaca gttagaagtt ttctatcagg aaaatcagat atagaagctt ttgaaagaag
     5401 attagctcca tcaactataa aaaaatctct tggtatattt gtaatcagta tatcagcagt
     5461 tatttttgga acattgatta tttcaataac tcagcctaat tttacattag ttcaatcagc
     5521 ttttgaggtt acatcagcac ttgctacagt tggttcaagt ttagctggaa ctccaaactt
     5581 aaatgcttta ggtaagatta taataataat ctttatgttt atgggtagag ttggttctct
     5641 tactttattt atggctatac tttctggtgg tagaagaaag agtcaaccaa tcagatatgc
     5701 agaaggtaaa attatggttg gttaagttac caaggagtag tataaattta taatgggttt
     5761 agagtagcac tgtaaatctg ttgataaaat aaatgagcct agatgtaatt atatgaagaa
     5821 ataaaatcaa ctaatgaaca aaattaatta atgaaataaa aaaacaggga aataaaatac
     5881 aaataaaaag gtaaatcaaa attaaattaa tctgtgtgta taaaattata tattgtgtat
     5941 aagttttgta tgtgtaaagt atataagttt tgtatgtgta aacaattaat ttaagagata
     6001 ttatataaat tgtgtattag ggaggaagtt tcgagatgaa gcaatatata gtcattgggt
     6061 gtgggagatt tggaagttca gttgcgtcta ctatgcatct tttaggacat caagtaatgg
     6121 caatagacaa aaatgaagat tcagttcaaa gtatatctga caaggtaacc cattcactta
     6181 tagtggatgt tactgatgag caagcgttaa ggtcattagg tttaggtaac tttgatgtag
     6241 cagtagttgc aataggttct gatataaggg catctataat ggcgactctt atagccaaag
     6301 aaatgggtgt agagttgata atatgtaagg caaaggatga attacaagct aaagttcttt
     6361 ataaaattgg tgcagataga gttgtatttc cagaaagaga tatgggagta agagttgcac
     6421 acaatttagt ttcggataat atattagacc atattgaact tgacccagag tattcaattg
     6481 ttgaaatcgt aactccaaat agttgggttg gcaagacact tatagagctt gaattaagag
     6541 ctagatatga gataactgta cttgctataa aaacaggtaa aaatataaat gttacacctt
     6601 ctccagatga ggaacttaca gccggaagta tcctagttat aatcggtcaa aatactagta
     6661 taacagcgat aacatctgga aataagggga taattagaag aagataattt actatttaat
     6721 atatatttaa ttgcaatgaa agcagatagt atcatataat tatgaatagt tatatgatac
     6781 tattttttta atcgaaggta gtatttattt gtaagattag ataaagagaa gttaaattaa
     6841 aagtaaggag gctgtgctaa tataaaaatt tataagttat tagcatttag ataaaatgat
     6901 tacaaatata aacagtaagg ataatgaaaa gttaaagtat acaagagcac tattaaaatc
     6961 aaagaatagg aataaagagt caaagttcat aatagaagga tacagaatag taatgcttgc
     7021 acttgaatgt atggcaaacc ttgattatgt atttatcaat gaagaatttg aaaataagaa
     7081 agaacatgta aaactattag aagatttgga taaaaaaaac acaaagatat acaagactac
     7141 taataaaaac tttaaagaat tagtggatac agaaaatact caaggaataa taggtgtagt
     7201 ttcatttaag aaaaaaaaat taagtgaaag tataaataaa aaagataaat ttgtattgat
     7261 tttagacaga atacaagacc caggaaatat ggggactata ataaggactg ctgattctgc
     7321 tggagtagat gccataatag cactaaaggg atgtgtcgat atatacaacc caaaagtaat
     7381 taggtctact atgggttcta tttttgatat gaatataatt gatgcttcac aagatgaaac
     7441 tgtggacatg cttaaatcat tggattttaa tatagtttca agttacttaa atacagaaaa
     7501 tttttatgac aaaatagatt atggctcaaa agtagcattg gtgataggaa acgaagcaaa
     7561 tggaataaat gaagaacttg tatcaaagtc tgatattttg gttaagatac ctatatatgg
     7621 taaagccgag tcgttaaatg ctgcgataag ctctgctata ctgatgtatg aaataaaaaa
     7681 atacttaatt taatgtattg taataaatat aatgtcatgt tataatcttg aattaaatag
     7741 tagagtatca aaaatagtca atatatatta a
//
LOCUS       DAYATN010000160         7655 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.162, whole genome shotgun sequence.
ACCESSION   DAYATN010000160 DAYATN010000000
VERSION     DAYATN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.162"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            117..242
                     /locus_tag="ACGI8A_003295"
                     /pseudo
     CDS             117..242
                     /locus_tag="ACGI8A_003295"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
     gene            297..563
                     /locus_tag="ACGI8A_003296"
     CDS             297..563
                     /locus_tag="ACGI8A_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFH0181260.1"
                     /translation="MAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDYLIKVL
                     AEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            612..1196
                     /locus_tag="ACGI8A_003297"
     CDS             612..1196
                     /locus_tag="ACGI8A_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HFH0181261.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1201..1524)
                     /locus_tag="ACGI8A_003298"
     CDS             complement(1201..1524)
                     /locus_tag="ACGI8A_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0181262.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1676..2194
                     /locus_tag="ACGI8A_003299"
     CDS             1676..2194
                     /locus_tag="ACGI8A_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0181263.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2370..3038)
                     /locus_tag="ACGI8A_003300"
     CDS             complement(2370..3038)
                     /locus_tag="ACGI8A_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFH0181264.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3153..4568
                     /locus_tag="ACGI8A_003301"
     CDS             3153..4568
                     /locus_tag="ACGI8A_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFH0181265.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4767..5249
                     /locus_tag="ACGI8A_003302"
     CDS             4767..5249
                     /locus_tag="ACGI8A_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0181266.1"
                     /translation="MKTNTDVIMEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFT
                     NVVGCTIHQYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQ
                     VYRNKDEFYPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA
                     "
     gene            5246..5830
                     /gene="clpP"
                     /locus_tag="ACGI8A_003303"
     CDS             5246..5830
                     /gene="clpP"
                     /locus_tag="ACGI8A_003303"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFH0181267.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6142..7218
                     /locus_tag="ACGI8A_003304"
     CDS             6142..7218
                     /locus_tag="ACGI8A_003304"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0181268.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTDGGFKSDDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7297..>7655
                     /locus_tag="ACGI8A_003305"
     CDS             7297..>7655
                     /locus_tag="ACGI8A_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181269.1"
                     /translation="MSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHCCPPS
                     CVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLRGPTG
                     ATGATGPTGATGAIGFGV"
BASE COUNT         2907 a          896 c         1289 g         2563 t
ORIGIN      
        1 ggaaaaataa catatttgta ttaaatttta ttgaaatgat taaatttatt tacttaatgt
       61 aaattattag ctaaaattgt attaaataat taaggaggtg agtagctctg gatgaaatag
      121 atttaaaaat aattaattct ctaaaaaaga atagtagggc ttcaacctct gaaataagca
      181 gacaaattaa tttatcagta ccagcagtct cagagcgtat aaggaaaatt gagcagtcaa
      241 atttaattca aaagtacact attcaaatta atcgggaaaa aacaaaatat aagcttttgg
      301 cttttatatt tatcaatact gatttatcta aaagtatgaa agaatttaag aaaactatat
      361 tggaatatga tactgtatta gaatgtcatc atatttcagg agagtatgat tacttaataa
      421 aagtattagc agaagataca agtaatcttg aagagtttat atcagtgact ttgaaggaaa
      481 ttggggggat tcaaaaagct aatacaacca ttgttttatc tacaattaaa gaagaattta
      541 atacaataaa agatattttt taaataattt actagtaaag tgttaattat attttaggag
      601 gaaaatagaa tatgaaagtt ttagtttttt tagcaaaagg ttttgaaact atggaattta
      661 gtgtatttgt agatgttatg ggatgggctc gtaacgatta tgggcatgat attgatgtag
      721 tgacttgtgg atttaaaaaa caagttatga gcacatttaa tatacaagta ttagttgata
      781 agacgataga agaagtatgt gttgatgatt atgatgctct agctattcct gggggatttg
      841 aagaatttgg attttatgat gaagcatatg attcatcttt tttaaatcta ataagggagt
      901 ttaattcaaa agaaaaaata atcgcttcta tttgtgtggc agcattgcca gttggaaaaa
      961 gtggtgtttt aaaaaatcgt aaagctacta catatcattt gaaaaatggt aaaagacaaa
     1021 gacagttaag tgagtttgat gtaaatgtag ttaatgaacc aattgttgta gataaaaata
     1081 ttataacctc atattgtcca gaaacagctc cacatgtagc ttttaagctt ttagaaatgt
     1141 tgacatctaa agaacaaatg gatgaagtta aattagcaat gggatttaaa ctgtaatggt
     1201 ttatagattt tctaagtaat cagaacccca gtcacacatg gaatctaaaa ttggaataat
     1261 actctttcca aaatcagata gcgagtattc tacttttgga ggaacaacag gataaaccac
     1321 tcttataatt aaaccatctg attctaattc acgtaattgt tgagttaaca tcttaggcgt
     1381 tgctttagga attaattttt ttaattcatt gaacctcaat atagtgtctt taagatgcca
     1441 aagtataagt gtcttatatt taccaccaat caatgcaagt gttgcttcta ttgggcaact
     1501 gtaattatct aaacattttt tcatttttgg gctcctttat ttattttaaa gtatcttttt
     1561 aggtactata ttacaaaaaa gtgcattatt gtaatatagt accatagtga taaaattata
     1621 gcataggtta aataaatcat caacttagcc taataaaaca ataggaggta aataaatgaa
     1681 tttcgttgaa cttgcaaaaa aaagatattc ttgccgtaat tatcaagata gaaaggttga
     1741 aaaagaaaaa cttgagaagg ttttagatgt tgcaagaata gctccaactg gtggtaatcg
     1801 tcaacctcaa agattgattg ttattcagga aaaagaaggt ataaataaac tttctaaggc
     1861 tgccaatata tatgatgctc cattagcaat tcttgtatgt ggagataaag acaaagtttg
     1921 gacaagacct tttgatggga aacaacttac agatattgac acaagtatag ttacagacca
     1981 tatgatgtta caggcaactg aacttggatt ggcaagtgtt tgggtgtgct attttaatcc
     2041 agatataatt agagaagagt ttagtttgcc agataatcta gaaccaataa atatattatt
     2101 gatgggatat gaatctaaga taccagagag cccagagaga catgaaaaaa ctcgtgttcc
     2161 tttaagtgaa attgtttctt atgaaactct ttaattataa aattaagtta ttcataggat
     2221 aaatgtaata aagtgtttat tataaacaaa attcaaaata aataatatct acatataatt
     2281 aattttagtg aagtatatct agtttaacta gaatgtatac ttcactaatt tatttattca
     2341 aaatatttat gaggatttta atatattaac taaaatatat caattgaatt aacttttaaa
     2401 tctggaaaag cacttgaaat atagtgtttt atgtcattta aatggtcttg aatttgcttt
     2461 tctgatattt tatcaggtcc atatacaaaa tacataatat ttttagtatc ttttgttata
     2521 gctgttcgat tatcagggcc attaagaata ctagataata taccaatatt atctgataaa
     2581 aataaatcat cttttgttaa aatatgttct ttaccaccta taccataaaa atgttcactt
     2641 ccatttgcta attctatttt caaaggtaat tccattttat caaggtcatg acctgctgtt
     2701 agaagaagat ttttcaattc tgctaaaaac attgactcaa ttgttacacc tacatctggg
     2761 ataccttttg attttaatag aatagattct aattgaagca atacagggta agttttcttg
     2821 aattttttat aatactttat ataacttgaa acaggctctg ttttaatttt ttctttacga
     2881 ttgtattctg ggtgttgttc tataatattt ttaattgtag agtttttaag aactaaaaaa
     2941 ttttctttat taggttttga atgtgtaata tttataacca taactccaaa tttaatattg
     3001 ggataaattt ctttaacttt atgtgatatt tctaacataa tatagactcc tttttatttt
     3061 atattttatg atataatctt agcaaattaa aagtggattt tgtagttcca tttttatagt
     3121 aattttatag aaccactttt ggaggtatta gaatgtggat tacgattaat agaaatagta
     3181 atatatcatt atcaagacaa atttattgtg agataaaaga tatgattctg gatggaagct
     3241 taaagtttga gcaaaaactg ccatcatcta ggactttatc aaaagaacta tctgtatctc
     3301 gtaatacagt tttagatgca tataatcaat taattgcaga aggatatttg gaaacaagga
     3361 atggatatgg aacaattgta gctaaaggaa tatcagggca taaagttgat tttaagttaa
     3421 atgatacttt tagagatgaa aaagatttac atactaataa acaaaaaaat tatatagatt
     3481 ttcgttcagg agttccagaa ttaaaactat ttccacgcaa agaactttca aagctatata
     3541 aacaaatact gtatgattta tcagattcgg atttcagata tggaagtaca gctggtgtat
     3601 gggttttaag agtggaaata tctaagtatt tatttcgtac tagaggaatt agatgtaatc
     3661 ccaaaaacat tatgattgtt tctggttcaa cacaaggttt atctttaatt tcacagcttt
     3721 tatacaaaag taataaaaag gttgctgtag aagaccctat tcataaaggc ttattaaagg
     3781 ttatttcatc tattggatat tcagttgttg gaattaaagt agatgataag ggaatgaata
     3841 ccaacttaat aaaatcaata aaggggattt cttttgttta tactacacct tctcatcaat
     3901 acccattagg agggatttta cctattcaaa gaagactaga gcttattaaa tgtgcaacag
     3961 agaatgatta ctatataatt gaagatgatt atgatagtga atttagatat gagggtcaac
     4021 caattagttc tctttatgaa cttagtccaa ataaagtaat ttacataggt tcttttagta
     4081 aaatattatc tccagctatt cgtgttggat ttatgttatt accagacaat attttagaaa
     4141 gctataaaac cttaaaaatg tactctgatg ttcatacaga aacaatattg caacatgtat
     4201 tagctgaatt tattggaaat ggaggtttag agaagtacat atttagaatg aagaagttgt
     4261 acaatagaaa acgtaagtac ctcataaaag ttttaaacac ggaatttaaa aatgaattta
     4321 aaataaaagg tgaagcagca gggcttcata tagttgctca tttctataat gttaaattta
     4381 cggaaagttt aattcataaa atttttgaag caggtataaa agtttatcct atacaacaat
     4441 atgcaattga gaatatatta gaatatgact atgaaattat actaggttat gctcatctta
     4501 gttttgatga aattgaaaaa ggtgtaaaaa tactcagcaa aataatacat atgaatcaag
     4561 gtacatagat aaaacatgta gcaatctaca tataatagat gtaattaagt ttaaacttta
     4621 aaagaaatat atattaatat ttttgtataa aaatacctga tattttttta tcgatacttt
     4681 attgctatac actaaattaa gatagcaatt tatattaaga aaaaactttt tgcataggtt
     4741 ataatactta aaaggagagg atagagttga aaacaaatac agatgttatt atggagataa
     4801 ttgattatat agaagaaaat cttacagaaa atctaaattt agataaaata gcttatattg
     4861 ctaattattc aaaataccat ttacacagaa tgtttacaaa tgttgtagga tgtactatcc
     4921 atcaatacat acagaggagg agacttactg aagcagctag acaacttata tttacagaca
     4981 aatatattat agacatagct attacagtag gatattctac acatcaatct tttacactag
     5041 catttagaaa tttatataaa gaatctccac aagtatatag aaataaagat gaattttatc
     5101 ctattcaaca gagatttgtt ttaaatctaa atcatcaaat tcaaagtgaa aaatgtattt
     5161 cttatgcaaa tatgaattat aaacaaagaa gagatgaaat caattcttat ctgaacttta
     5221 atattaattg tgaggtgaaa gtagcatgag tttagtgcca catgtaattg aacaaacagg
     5281 tcaaggggaa aggtcttatg atatatattc aaaattatta aaagatagaa ttatatttat
     5341 aggagaagaa attaatgatg ctattgcaag tttagtagta gctcaattac tgtttttaga
     5401 atcagaagac ccagattcag atataattat atatataaat agccctggtg gctctgctac
     5461 atcaggattt gctatatatg atactatgaa ttatgtcaga tgtgatgtat cgactatttg
     5521 tattggtatg gctgcaagca tgagtgcttt tttacttgca ggaggaacac atggaaaaag
     5581 atgtgcttta cccaattcag agattatgat tcatcagcca atgggaggag ctaaaggtca
     5641 agcaacagat gtaaaaatag ctgtagataa tatattgaaa ataaaagaac gattagataa
     5701 aattctttct gaaaatacag gtaaatcaat agaagatatt aggagagata cagatagaga
     5761 taattttatg actgcactag aggctaaaga gtacgggctg attgattata taatgaatag
     5821 gaatgaataa agtatcaaag tatcatatta aaatgtctgt tttacaaata tatatataat
     5881 attaaataag agtgtctcaa aatgaacttt ttagtttatg ggacactctt ttttgtatga
     5941 aattaaatat ggttttatca tttcgacaat tattaccaaa tttcaagttt aataaattct
     6001 actataaaaa atacataaaa gataaggttc tttagtaact ttctgtcgtc aaaagttttt
     6061 ataaatttaa taacaaaaca agagaaaata ccatatatta cttatgtctg caatatatag
     6121 aagacaaagg agttgtacat tatgattaca ataagtttgt gcatgattgt taagaatgag
     6181 gaagaagtta taggcagatg cttagaatgt gtaaaggata tagtagacga aattataatt
     6241 gtagataccg gttctacaga ttccacaaaa gaaatagtct caaaatatac agacatggtg
     6301 tacgatttcg aatggataga tgatttctct gctgcaagaa atttttcatt ttctaaagct
     6361 tctaaagact atactatgtg gcttgatgca gatgacataa ttttagaagc tgatagagaa
     6421 aaattattaa agattaaaga aagcttggat acttccattg atattgttat ggcaaaatac
     6481 aatgtatcct ttgatgaaaa tggtaaccca actctctcat attatagaga acgattgttt
     6541 aaaagaagtt ctaatcataa atgggttgac cctattcatg aagtagtacc tctttttgga
     6601 gaggtatttt attctgacat tgcaatatct cataaaaagc ttcatagaca ggaccctcta
     6661 agaaatctta gaatttttga aaaaatgatt tcagaaggaa aaacattgga gccaagacac
     6721 caattctatt attctagaga gttgtactat aatgctaggt ataaagaagc aatagaaggt
     6781 tttactaagt ttttagactc atcaagagga tggattgagg attgtatcag tgcttgcaga
     6841 gatttagcca catgttatta cttaattaat gatgaaaaaa gtgccttata ttcattgttt
     6901 agaagtttcg aatttgatga gccaagagca gaaatttgtt gtgacatagg aaagcatatg
     6961 tttgatagac aaaaatataa agaagccatt ttttggtata aggtagcatt aacaagagat
     7021 aagaatgata ctgatggagg ctttaagtca gatgattgtt atggatatat accatctata
     7081 caattaagtg tctgttatga caggttagga gaatcagata aagctattta ttatcatgaa
     7141 aaaacaaaag aaatcaaacc taatgatagt gctgttttgc acaatgaaaa ttattttagt
     7201 aaatttaaaa atagttgatg ggtcacaatc atactaaatt tgacatatta tatatagagc
     7261 ttaaaagctc aaatatatca ggaggtgctt ttaataatga gtagaaataa atattttgga
     7321 ccatttgatg ataatgatta caacaatggc tatgataaat atgatgattg taacaatggt
     7381 cgtgatgatt ataatagctg tgattgccat cattgctgtc caccatcatg tgtaggtcca
     7441 acaggcccaa tgggtccaag aggtagaacc ggcccaacag gtccaacggg tccaacaggt
     7501 ccaggtgtag gggcaacagg cccaacagga ccaaccggtc cgactggtcc aacaggaaat
     7561 acagggaata caggagcaac aggattaaga ggtccaacag gagcaacagg ggcaacaggc
     7621 ccaacaggag cgacaggagc tataggattt ggagt
//
LOCUS       DAYATN010000161         7614 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.163, whole genome shotgun sequence.
ACCESSION   DAYATN010000161 DAYATN010000000
VERSION     DAYATN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.163"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            232..1425
                     /gene="nifV"
                     /locus_tag="ACGI8A_003306"
     CDS             232..1425
                     /gene="nifV"
                     /locus_tag="ACGI8A_003306"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HFH0181270.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1450..3375
                     /locus_tag="ACGI8A_003307"
     CDS             1450..3375
                     /locus_tag="ACGI8A_003307"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HFH0181271.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3397..4392
                     /locus_tag="ACGI8A_003308"
     CDS             3397..4392
                     /locus_tag="ACGI8A_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HFH0181272.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4717..5190
                     /locus_tag="ACGI8A_003309"
     CDS             4717..5190
                     /locus_tag="ACGI8A_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFH0181273.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5224..5718
                     /locus_tag="ACGI8A_003310"
     CDS             5224..5718
                     /locus_tag="ACGI8A_003310"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0181274.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5815..6336
                     /locus_tag="ACGI8A_003311"
     CDS             5815..6336
                     /locus_tag="ACGI8A_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0181275.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            6525..6836
                     /locus_tag="ACGI8A_003312"
     CDS             6525..6836
                     /locus_tag="ACGI8A_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFH0181276.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6864..7550
                     /locus_tag="ACGI8A_003313"
     CDS             6864..7550
                     /locus_tag="ACGI8A_003313"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFH0181277.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         3048 a          759 c         1484 g         2323 t
ORIGIN      
        1 aaaacataat aactttcatt tttaagaagt tttcacaaaa aagaaaaaat atagtgcaat
       61 aaatgataca aagtaatata atagattaca agatatgaaa agctaaaaaa ataaattgca
      121 aatgaaatta aaatgaataa aaaataaaaa aataatcata agttagaagt atcaatgaaa
      181 aataagactt aatgttttta aaattaaaag tatactaagg ggggcattaa tatgtgcgtt
      241 atatcaaaag atagagcaaa ggaaataaaa atagtagata ctactttaag agatggagag
      301 caaacagctg gggttgtatt tgcaaataga gaaaaaataa tgattgctga aatgttaagt
      361 gacctaggtg ttgaccaaat tgaagttggt atacctacta tgggtggaga tgaaaaaaat
      421 gtaataaagc acatatgtag tagaaattta aaatcagata taatggcttg gaatagagca
      481 gtaataaaag atgttgaaga atctatttct tgtggtgttg atgcagtagc tatatcaata
      541 tcagtatctg acattcatat agaaaataaa ttgagaactt ctagaggctg ggttttagaa
      601 aatatggcta aaacagttga atttgcaaag aaaaatggat tatatgtttc agtaaatgga
      661 gaagatgctt caagagcaga tatagacttc ttaactgaat ttataaatgt tggaaaacaa
      721 gcaggggcag atagatttag atactgtgat acagtaggag ttatgaatcc tttttctata
      781 aagaatgcaa tagaaactct ttatgaaaga actaattttg atatagaaat gcatacacat
      841 aatgactttg ggatggcaac tgcaaatgca cttgcaggaa tagcagcagg agcaaattat
      901 gttggtgtta cagtaaatgg attaggtgaa agagcaggaa atgcagcatt agaagaagtt
      961 ttaatggcac ttaagtgtgt ttataaatgt gatttaaata atatagatac tagaaaattt
     1021 agaggaatat gtgagtatgt agcacaggca tcaggaagaa tacttcctac atggaaacca
     1081 gtagttgggg ataatatgtt tatacatgaa tctggtatac atgcagatgg agctttaaaa
     1141 gacccacata actatgaacc atttgaccca agtgaagtaa atcttgaaag aaaaatagta
     1201 ataggtaaac attcaggaag agctgcagtt gtaaataagt tgagtgaata tgaaatgtat
     1261 atttctccag aaaacgctac caagctatta aatgctataa gagctacatc tataagatta
     1321 aaaagaagtt taatggataa agaaattctt caattatatt gtgatatact agcacatgag
     1381 aaaggcacaa cagaagaaga agcagttaga ggttcatata tttaagatat aattgtttag
     1441 gaggaaaata tgggagataa tatagtttat aaaataatta aaaaacatat agtagatgga
     1501 gaagctgttg ctggaagttc aattggtata aaaatagacc aaactttaac acaagattct
     1561 actggaacaa tgacatatct tcaactagaa gctatgggaa tagataaagt aaagactaaa
     1621 agaagtgtgg catttgtaga ccataatatg ttacaacaag gatttgaaaa tgcagacgac
     1681 cataagtata tacaaacagt ggcagataaa tatggagttt atttctctaa gccaggaaat
     1741 ggtatttgtc atcaagtatt tttagaaaga ttttcaacac caggagatac acttttaggt
     1801 tctgatagcc acacacctac tgctggaggt gtgggcatga tggctattgg agctggtgga
     1861 ttagatgttg ctttagccat ggcaggagga gcatactata taaaagcacc aaaagtctgt
     1921 aaggtgaatc tagttggaaa attaaataat atggtatcat caaaagatat aatattagaa
     1981 gtgttaagaa aacaaactgt aaaaggtgga gtaggtaaag tttatgaata tggtggagaa
     2041 ggtgtcaaaa gcctatctgt tccacaaaga gctacaataa caaatatggg ggctgaactt
     2101 ggagcaacta cttcaatctt cccaagtgat gaaaaaacac tagaattttt taagagtcaa
     2161 ggaagagaag acgcatgggt agagctaaaa ccagatgcag atgcagttta tgatgaagag
     2221 attactataa acttggatga attaaaacca ttagctgcaa aacctcatag cccagacaat
     2281 gtagatgaag ttgaaaatat aggcaagatt aaaatagacc aagtggcaat aggaagttgt
     2341 acaaattctt cttatgaaga cttaatgaaa gtagcacaaa tcctgaaagg aaataaagta
     2401 cataaggatg tgagtttggt aatagcacca ggttcaagac aagttatgga gatgattgct
     2461 agaaatgggg ctttggcaga tataataagt gctggagcta gaatactaga aaattcttgt
     2521 ggtccatgta ttggtatggg tcaatctcca ggtactgact ctgtgtcttt aagaactttc
     2581 aatagaaatt tctatggtag aagtgggact ttgtcagcac aagtatattt agtaagtcca
     2641 gaagtggcag cagtaagtgc aatcaaggga gtgcttacag acccaagaga atttgatatt
     2701 aaatttacaa atttagatgt aaatgagttt ttgatagatg atagcatgat aattaaacct
     2761 gctgatgtag gaagtgatgt agaagtagtt agaggaccta atataaaacc tttccctctt
     2821 aatacagagc taagtcagtc tataggtgga aaagttatat taaagacaga agataatata
     2881 actactgacc atataatgcc atcaaatgct aagttacttc cttttagaag taatataccg
     2941 tatcttgcaa actattgttt caatactgta gatacagaat ttcctcagag agcaaaagac
     3001 aataatggtg gatttatagt tggtggagat aattatggtc aaggttcaag tagagaacat
     3061 gcagctttag caccattata tctaggtgtt aaaggggtaa tagttaagag ctttgcaaga
     3121 atccacaagg ctaacttgat aaatagtggt ataattccaa tggagttttg tgatgagaaa
     3181 gattatgaaa atatatctct tttagataat ctggagatac caaatatttt agacaatcta
     3241 ggtagtggaa ttttagaagt taagaataca actaaaggaa cttcttttaa ggtcaaagta
     3301 gaattgtcag caaaagaagt tgatgtatta aaagctggtg gaaaattaaa ctacactaaa
     3361 aatcaagcta attaatatga aaagagggaa tattgaatgt ataaagtgac acttatacca
     3421 ggagatggaa taggaccaga agttgcgaaa gctatgaaaa aggtagtaga agctactgga
     3481 gtagaaatag aatgggaaga agttaatgca ggagaggcag ttattgaaga gtatggtact
     3541 ccacttcctg aatatattat agatagtata aaaaagaaca aaatagctat aaaaggccca
     3601 attactactc ctgtaggtaa aggatttagg agtgtaaatg taactttaag acaggcatta
     3661 gatttgtatg tcaacttaag acctattaaa agttttaagg ggataaaatc aagatatgaa
     3721 gatgttgatt tagtggttgt aagagaaaat acagaagatt tatatgctgg aatagaacat
     3781 aaaataggtg attatgcagc tgaaagtata aagataataa caagaagtgc aagtgaaaga
     3841 atcgttgatt ttgcatgtaa ctatgttaaa gataataaga gaaaaaaagt tacagctata
     3901 cataaggcaa atataatgaa aatgtctgat ggattgtttt tagatgtgtt tagagaagtt
     3961 gcatcaaaac atggtgtaga atatgatgat ttgattgtag atgcagcagc tatgaactta
     4021 gttttaaatc cagaaaatta tgatgtaatg gtaatgccaa atctttatgg agatatacta
     4081 tcagatttag gtgctgggct tgttggaggt ttaggtataa ttccaagtgc aaatattggt
     4141 aaagattgtg cgatatttga agctgttcat ggaagtgctc cacaaatagc aggtcaaaat
     4201 aaggctaatc caacagcact tattcaatca tcagtaatga tgcttagata cttaggtgaa
     4261 tatgaaaatg cacaaaaaat agaaacagct ttagaaaaag tatttcttga aggaagtaaa
     4321 ttgactgtag atttaggtgg ctcagcttct actacagagt ttgcggatga ggtgtgtaag
     4381 tatattgtat agattttatt tagggaaaaa gtaaaatata gattgaagaa aagaggcttt
     4441 ctcaaaatag agataagcct tttttattgg aatgataaaa tatatttgat tttatacaaa
     4501 agagagttcc tcataaacga aaaagttaat tttgggatat ccattttaat tttaaaatta
     4561 attgattaat acgcaaagaa agattaaata aatttattat ttttaattga actttcaacc
     4621 aaaatatggt aaaatttaaa aagttgaact ttcaactaaa aatgaaaata ttgattatgt
     4681 gaaattctat aaaacagaaa ctatttgact ttagccatgg tagtttcagt ggaggtgtgt
     4741 ttgattaaga ctttagatag taatatatta agagaagtag gtactttatc tagagcagta
     4801 aattcaataa atgatattaa gtacaaggaa ttaaagttac aaaaaggaca atttacattt
     4861 ttgacaagaa tctgtgaaaa tcctggtata aatcttgtgg aattatcaaa tatgttaaag
     4921 gtagacaagg ctacaacaac caaggctata caaaaattaa taaaggcagg atatgtagat
     4981 aaaaagcagg ataagtttga taaaagagga tataatttaa ctccaactga taaatctttg
     5041 gaagtttatg agttgataat tgaagaagag aatagaagta tagagatatg ttttgataat
     5101 tttacagatg aagaaaaaca agtggtaact aagttattag aaaaaatgag caaaaacgta
     5161 gaaaatgaat ggtttaaagt aaagagatag taaataaata cattaactta aggagatatt
     5221 tttatgatta gaaaagcgaa tatgaatgat ttagaaagca ttatgaaaat tataaaatct
     5281 acagtggaag aaatgaagac ttataataat acacaatggg atgaaaacta tccattagaa
     5341 aaagactttg tttcagatat taagaaacaa gatttatata tttatgaagt ggatggagaa
     5401 gtagcagggt ttatatgtct taattatgaa gagccagaag aatatttagg cttaaattgg
     5461 tcatctaata aaaaagcaat ggtaatacat agaatggctg taaatccaaa ctttagaaag
     5521 acaggaatag catctaagtt agttgatttt gctgaaaaat tagctgtaga aaacaatgta
     5581 tcatacttaa agagcgatac atattcaata aactcaaaaa tgaatttatt acttacaaag
     5641 tgtggtttta taaaaatcgg agaaatgagt tttttaggaa aggagaaatc tttctactgt
     5701 tatgataaaa tattataaag agcatttata atacaagggg taaataatag attttcagca
     5761 ttagtaaggg cataatataa cctagtatat gctattatga aagggatgat ttatatgatt
     5821 ggatttctaa agaaaagaag aagtattaga aaatacaagg atgttgaagt agaaaaagaa
     5881 aaattagata aaatattaaa agctgctctt ttagctccat catcaaaggg gcttagaaca
     5941 tgggaattta tagtggtaga tgataaagaa aagctaatta atttatctca atgtagaact
     6001 aaaggtggtg gatttttctt aaaaaatgca cctttagcta ttgtaattat tgcagataaa
     6061 gaaaaaaatg atgtttggat agaggatgca agtatagctg cttcatacat acaacttcaa
     6121 gctcatgaac taggtttagg ttcttgttgg atacaagtaa gaaatagaat gcatgatgat
     6181 aatatagaag ctgataagta cattagagaa gaattaaaag tacctagcaa gtacagtgta
     6241 gaatgtataa tttctatagg atatcctgat gaagaaaaga aagcatacgg tgactctgat
     6301 ttagactata aaaaggttca ttttaacaac ttttagtata taatatgttg tattaagaca
     6361 ttatattttt ttctattgtt aatatatact taaaattgta aaaatatatc tgaattttac
     6421 taacttatct aggggttatg tacaaggatt ttaaagataa atatagaaat taatgtttat
     6481 aatcgataca taacttgagt aacaaaattt aaggaggtta aagtatgggt atatttaaaa
     6541 caaaaatgga tgaggactgg aaagtaaact acattaaaga gtttaatgaa atgagagatt
     6601 cttatgagag taaacttcaa aaaaaacagt ttgaggtaga tagtttgaaa tcagaattgg
     6661 atagactaag aagctataaa aatagcctta agccaaaaga aaaacaaatt actgatgatg
     6721 atataaataa tataaagagt ttaagaagag atggcttaag ctataaagaa atttcaaatc
     6781 aaacaagttg gagcaaagca actgtaagta gggtattaaa tggactttac gattagcata
     6841 tacatggggt gatttaaatt gggatgaagt atagttacaa aaaatgcata atagattcta
     6901 ttttattgat gtttatagtt caaatattaa ggatgatttt gaattatgta cttctaagtc
     6961 aatttgaatt tactcttgaa aattttaata ttataaactt aatttctttt acattagttg
     7021 gtttatcgtt aattttattt ttaaaagata attctttgta taacaaggtt agaaatagaa
     7081 aaatcacaga agcttttgaa gaaaataaaa acaatatact tattgaaaag tgtaagctga
     7141 tactttttgt tgtggtttta tctttagcaa taattgtaac ttattgtact aagggatatg
     7201 tactttttaa tgtaactatg atgactttgt ctgtattgat agtcccaata tttgaagagt
     7261 tattttttag agaatatata tggaactatc taagtaattt tataaagtca aagagtaaaa
     7321 ttatctgtat aacatctata ctatctggaa tttataatat aggatacata gatgttataa
     7381 gaaattatgt aatattgtat aacaattcaa gctacacctt tgaagttatt atttcaaaaa
     7441 ttatgatagg tactgttttt ggtattgtac ttggattagt taagtatagg tttagagatg
     7501 tagggttttg tatattattg aggagcttat ttgctatatt tattaggtag atagtataat
     7561 cggtttataa ggttgtttta aatttaattt taaatcaacc ttattttatt tgtg
//
LOCUS       DAYATN010000162         7546 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.164, whole genome shotgun sequence.
ACCESSION   DAYATN010000162 DAYATN010000000
VERSION     DAYATN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.164"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            317..1618
                     /locus_tag="ACGI8A_003314"
     CDS             317..1618
                     /locus_tag="ACGI8A_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFH0181278.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1694..1969
                     /locus_tag="ACGI8A_003315"
     CDS             1694..1969
                     /locus_tag="ACGI8A_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFH0181279.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3140..3370
                     /locus_tag="ACGI8A_003316"
     CDS             3140..3370
                     /locus_tag="ACGI8A_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0181280.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3363..3944
                     /locus_tag="ACGI8A_003317"
     CDS             3363..3944
                     /locus_tag="ACGI8A_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0181281.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4523..5200)
                     /locus_tag="ACGI8A_003318"
     CDS             complement(4523..5200)
                     /locus_tag="ACGI8A_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFH0181282.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5389..6753)
                     /locus_tag="ACGI8A_003319"
     CDS             complement(5389..6753)
                     /locus_tag="ACGI8A_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0181283.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            6881..7402
                     /locus_tag="ACGI8A_003320"
     CDS             6881..7402
                     /locus_tag="ACGI8A_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFH0181284.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         2946 a          867 c         1293 g         2440 t
ORIGIN      
        1 atgaattata ttgaacgata tatcaataat tggcagtaag ttatatcaaa taagatatta
       61 ctatcgagta atgaattata ttgaacaata tattaataat tggcagtaag ttatatcaaa
      121 taagatattg ctattatgta gtaaattata taaaacaatg tatcagaata aatagtatat
      181 taataatttg tagtatatca tgacgaataa tacagtcata acaaatagtg agtcataaca
      241 aataatgtat ttataattga taatgtattt aaaaatcaaa atataaaaac ttataaggaa
      301 agagagatta atttatatgg gtaaatcaaa agatgttgaa atagagaagt ctattttagg
      361 cacaattctt ttagacaata aattaagcta taagctagat gaattaaatg aaaacatgtt
      421 tatgaatgat atatgtcttg agatatttaa aattatgaaa gaacttaaaa aagaaaacat
      481 agtgattgat gtggccactg tgaaatcaaa gatagataga aaatctctag caataaaaac
      541 aagtgatgtg actaatctaa ttacttgggg tcagaatttt ggtcttgatg gtcatattaa
      601 aattttaaaa gaaaatttag ctagaagaag cataaatcaa aactgccaaa accttttaca
      661 cagcctaaac cttggagaaa atatcgatac ttgtatttac aaatttgaaa gtaatataaa
      721 ggagattttg gacaaggata cttatgaaaa tgatgatgta aatagtatag cagggaaggt
      781 gcttgatttt ttagaaaaca aaaaagatat tggatttaaa tttggtataa aattacttga
      841 taccactata ggaggtcttt ttaaaggaga gctaaccaca atagcagcta aatcgggggt
      901 tgggaagact gctttggcac ttcaaataat gttaaattca tttaagcaag gtaaaaaaac
      961 tttatttata agtagagaaa tgaccagtga gcaagtattt atgagaaata tatgcagagt
     1021 aactggtgta agtacaagga atatgaaatc taaagaaata gatgaaaatg attggaaact
     1081 tattgtaaat gcgataggtg atttaagcga aaataactta atttatatca atgataagat
     1141 agatacaatt agtgcaatta gaaagaggat aaggcaggta aagccagatt tattaatagt
     1201 ggattatgtg caactcctta catctcagaa aagcatggat aagagagaaa gggaggtagc
     1261 tactttttcg agagaactta aaaatatgac tttagatttt aatatacctg taatccaatt
     1321 aagtcagtta aatgatgaaa tgaaagattc aagaccttat ggagatagac ctatgaggga
     1381 tagcaaagcc atctaccacg atagcaacaa tgtggtttat atacatcaat taaaggggag
     1441 tgattatgaa gaagcagtga gagatattgg agaaagtgag gaggcagtta gagcatcaga
     1501 atacagggga attaagatgg ttgatttaat agttgcgaag tgtagagatg gacagactag
     1561 gcataagcat ttttgttact ttggggataa gttacatttt caagaactaa attattaggt
     1621 caataaagta gtaataacaa gcttattaaa agtcgggaaa tagcaaatag attataaaat
     1681 atgaggtgaa aatatgttgg aattaagaaa aattatagaa ttacttgaag aacacaagat
     1741 agaagtaagt attccaaagg agttacataa gtatagttta ttggaatacc ttgaattgtg
     1801 tcaacaaagt gggggaacat atttttatat taaaacactg gaaggggctt taaaagaggc
     1861 tagaaatgag cttatgatta aattgtttaa tgggaaaaat tatatggaat tagcgaggat
     1921 gtttgaaatg agtgttatta atgtcaggca tattgttaga aaaaaataga tatttttgga
     1981 gaatacaagt gtgatatact tctgaaatag aagttattat aagaggagta taagtgtgat
     2041 taacaacatg attttgtgaa taaaattgag gatatatatg agttaaatgt gtgattaata
     2101 aggtaagatt gtgtataaga ttatgagtat ttaaaaggag gggctataat taatagtgta
     2161 gagttgtgtg tttgcttaag attatataat aatttaatag attaaactat actaataagg
     2221 taaaatcgtg tgtttagtta aagcttatta taaaatcttg atatgtgatt aatgatgcaa
     2281 tatgtgaata aagttaagat tataatagat ttaacctgtg gctaatagaa taaaattgtg
     2341 aataaagtta agattataat aggtttaaat tgtgactaat agagtaagag tgtaaataga
     2401 gataaacttc aatatgagct taacctatga ttaatagagt aagaatgtaa atagagataa
     2461 acttcaatat gagtttaact tgtggctaat agagtaagag tgtaactaaa gataaacttc
     2521 aatatgggtt taacttgtgg ttaatagagt aagaatgtaa atagagataa acttcaatat
     2581 gagcttaact tgtgattaat agagtaagag tgtaaataga gataaacttc aatatgggat
     2641 taacttgtga ttaatagagt aagagtgtaa ctaaagataa acttcaatat gagcttaacc
     2701 tatgattaat agagtaagaa tgtaaataga gataaacttc aatatgggtt taacctgtgg
     2761 ttaatagagt agggtgtaac taaagataaa cttcaatatg agtttaactt gtggctaata
     2821 gaataagatt gtgaataaag ttatagtttt agatgggtat taacctgtta ctaatagaat
     2881 atgattaggt gtaaaattat agagctgcat aagaaaatat tatagatata ttaaatatta
     2941 gataataaat atttatatag atacacaaaa gaaaaggcga tatgaagcta aacttaatga
     3001 cttcatatcg ttttttattt gaaactcctt tgtatttaga gtgtgatttt taatattgag
     3061 gttttatgtg attaagtcac agataaaaag taagtatctt gatataattc ttatagatta
     3121 ataatatagg gaggttatta tgtctaataa aaaaaagaag tatgcaaata ttttagcaaa
     3181 tgagtgcgtt gcttgtggta gctgtataaa agcatgtcca agaagtgcta tctcagttcc
     3241 atgtggcatt agtgcaaaaa ttaatagaga tttatgtgtt ggatgtggga tttgtgaaaa
     3301 gatatgtcca gcatctgtaa ttgagattat aacaatatta aaagaggaag aaggtaagtg
     3361 tcatgaataa taaaaaacat tggtatgact atttatggat tttttcttca atatatttga
     3421 tattgggatt tgtaaatatt attttcgctt ggattgggct aatttgtttc tttgttccac
     3481 tagctatttc tattgttaag ggaaataaag cttactgtaa taagtattgt ggaagagggc
     3541 aactgtttaa tttgcttgga aataaattga agctgtcaag aaatagagat attcctaaat
     3601 ttattagaag taagtggttt agatatgggt ttttgacatt ttttatggta atgtttgcca
     3661 atatgctttt ttcaacttat ttagtgtttg aaggaagtag aaattttaaa gaagtagtga
     3721 ctttgttgtg gactataaaa atgccttggc aatgggcgta tcatggaact ttagtttcac
     3781 cttgggtggt acagtttgca tttggttttt atagtgttat gcttacatca actgttcttg
     3841 gtttgattac tatgatattg tttaaacctc gttcatggtg tgtttattgc cctatgggta
     3901 ctatgactca gatgatatgt aaggcaaaga gtaagtctgt atagaaaggc cttatgtgat
     3961 atttacagtt ttgttcaagt agagatgagt agtaggtttg aatatatatg acaaatatat
     4021 gagtataggg gcatagattt agtttgtgta aggtaaactg atttatgctt cttttttatg
     4081 tcttagatta ttggctaagt ggaaacaatg aatttagata ttaagaatta ttcagttatt
     4141 tgaagaaaat agaataataa ttaacattat attaatggat attcattaca tataagccag
     4201 ttaatagcaa gatttattta atatgatata ggtcaagtat ttaaactaaa taaggtcatg
     4261 acaattaata taagtataga tataaaaaga ttagaagtta taacttataa aaggtattct
     4321 atataaatag tatggggaaa atagtcaaat taacttatta gaaacaaaaa atatacataa
     4381 aaaataaatg taaatatcaa ataagatact atatcaaaat agatataagt atctttttta
     4441 ttattgaaat tatgaaaata tatttgattt tacacaaaaa agagtgcctc atgaactaaa
     4501 aagttcattt tgagacacac ttttacagtg tcaaagaacg aagtttatcc ttatccaata
     4561 cctttatacc accacgagat aaagacacaa taccttctct agcaaaatat ttcaacattc
     4621 tcgatacaac ttctctggca ctacccatat acttagcaat ttgctcatga gtcatattta
     4681 tagtgctact gccattttta gctatctcat caattaaaaa agatgccaaa cgcttatcaa
     4741 aactcataaa aagaatttgt tgcatagccc acataacatc agaaaaacgt tcagtagcta
     4801 atttatatga aaataactct gcatgaatat tttctgtaga taattttgca aaaacagagg
     4861 aactaatctg tataatatcg cagtccgttt cagcatctac atagacatca aaagttattg
     4921 tttttagaat acaggaagct gataaaatac atacatctcc atcatcaagt cggaacagtg
     4981 taacctctcg accttcatct gaaagtatgt atgtacgcac tgttccagat tttataatca
     5041 aaataccaat acagtcacta tcaccacaat gtactggatt cccttttttg taatgcgagg
     5101 tatttgctga agaaattaat aattcttttt gtaagtcagt aagtttatcc caaaaaggca
     5161 agctatgtgt aagtatatca atttcatttt gtgaaatcat acaatatctc ctttcttaaa
     5221 ttgttataat cccatgatac aagtgaatag ataaattgac aagtagtttg ttaagaggat
     5281 tatttagtca gatagtaata gtatgattta tgtagaaaaa atatgataat atatattctt
     5341 gctattaatg agaaagtaat atgttttgat gatagtaggt ttgtataact aatgcatacc
     5401 gtttattttt aaaagattaa gttccttatg taaccttata gacataaaac cagcaattat
     5461 agcagccatc aaatcagcta ttggttgtgc atataagata ccatttaacc catagaatac
     5521 aggaataatc aaaattatag ggataaaaca gacaccttgt ctacacatac ctaaaaatcc
     5581 accttccttt gcttttccta gtgcgaggaa taatgtagaa tacactgtat agaatccaaa
     5641 tagcataaat gaaaacccat ttgccctaag agctttacta ccattttcta ttaataaaat
     5701 gtcaccactt gtaaattgtg aaattataga ttttgggaag agtatcaata taagaccaag
     5761 cactacacaa aatactgtag tccataatat ggaagttttt attgctatat gtaatctatc
     5821 gtagttcttt gaaccaaaat tataaccagc aattggttgg aaacctttta taaaaccaaa
     5881 tacaacaaga ctacctaaag acgttattcg tgttacagca cccattccag ctatcacaga
     5941 atcaccatac tttgtagcct ccatattagt taatgtaata gcaagactag tcaataattg
     6001 aaatattaag gtaggtatgc caatttttag gatttctgaa aaaatttctt ttgaaaaaca
     6061 gcattcgtta attctaaaac taaatatact tttcttgctt aaaatatatc ctacataaac
     6121 aagtgttgaa gcaatctgtg agatagctgt tgcaatagaa gcacccctta cccccagacc
     6181 aaaagtataa atgaatatcg ggtctaaaat catattaagc aaagcaccta ataacatagc
     6241 acacatagtt gttttagcag ctccttcact tgtgacaata ttagtcatag tgacattgaa
     6301 tatattaaaa atagatgaca cgacataaat gctagcataa gatattgcat aaggcataat
     6361 actttttgta gctcctaaca attttaaaat tggattgaga aaaatcataa tcacaataat
     6421 aactaatgta cccactataa tactactgta aatagatgta cttaccactt ttcctgcttt
     6481 ttcattatca ccatggccta aaagtctggc aacatatgaa gcagctccat ttccaaacaa
     6541 taatcctaat cccacaacaa gctgacctaa tggaaatgca acggatatag ctcccatttg
     6601 acttgttcca agtccaccca caaagtatgc atcaaccaaa ttgtataatg cagttatcat
     6661 catacctatc atggtaggta atcccataga taacaatgct tttgagattg gggcagaccc
     6721 taataattca attcttttgt tactttcttt catacttaac actcctcttc tattgacatt
     6781 aagtctaaat tataatacta taaattatag taagtatgga aaatagtact ataatttata
     6841 gtatttgtca atagtaagaa aactaaagga gatgattttt atggctacaa tagatttaat
     6901 tgtattggga atagtaaagc aacaacccaa gagtgcttat gatattcaaa aggcagtgga
     6961 atacaggaat atatcacgtt gggttaaaat aagtacacca tcaatttata aaaaagtaat
     7021 tcaattagaa caaaagggat atattgaaag taaaactgtg aagaatggaa aaatgccaga
     7081 aaaagcagtt tattcattaa ctgaattagg tgagacacat tttttagatt taatgtttga
     7141 tacatctagc aaaccaataa atattctttt agattttaat gctgttatag taaatttaaa
     7201 tagccttaca ccagaaaaac agaaattatg tctcaataat attgaagaga atgttcaaga
     7261 gttaaagaaa actattgagg aaaatataag tataaagaca gaagtaccag acacagcaaa
     7321 agcagtgttg gagcaacagt tggtgctagc acaagcattg gaaacctgga taaattcttt
     7381 aaaagataat atgggaaatt agaattagag aagctaactt aataatcatt aaacaaaaac
     7441 cagcatattt aataatgaga tattagaaaa aataaaacaa atgatataaa ctgaaaagta
     7501 tatttaaaat aaagctcact taattgaggg agaatgttaa actttt
//
LOCUS       DAYATN010000163         7418 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.166, whole genome shotgun sequence.
ACCESSION   DAYATN010000163 DAYATN010000000
VERSION     DAYATN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.166"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            212..2242
                     /locus_tag="ACGI8A_003321"
     CDS             212..2242
                     /locus_tag="ACGI8A_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0181285.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            2239..2691
                     /locus_tag="ACGI8A_003322"
     CDS             2239..2691
                     /locus_tag="ACGI8A_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0181286.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            2693..3748
                     /locus_tag="ACGI8A_003323"
     CDS             2693..3748
                     /locus_tag="ACGI8A_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFH0181287.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            3815..4123
                     /locus_tag="ACGI8A_003324"
     CDS             3815..4123
                     /locus_tag="ACGI8A_003324"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFH0181288.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            4227..5492
                     /locus_tag="ACGI8A_003325"
     CDS             4227..5492
                     /locus_tag="ACGI8A_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HFH0181289.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            5580..6296
                     /locus_tag="ACGI8A_003326"
     CDS             5580..6296
                     /locus_tag="ACGI8A_003326"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFH0181290.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHELEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(6491..6784)
                     /locus_tag="ACGI8A_003327"
     CDS             complement(6491..6784)
                     /locus_tag="ACGI8A_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HFH0181291.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(6790..7005)
                     /locus_tag="ACGI8A_003328"
     CDS             complement(6790..7005)
                     /locus_tag="ACGI8A_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181292.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT         2820 a          757 c         1313 g         2528 t
ORIGIN      
        1 taacttgtct tttttattta tatatttaat aaaaatatat ttgcagattt tgtaaaaata
       61 attttcaaat gttgtaagtt gtggctaaag ctttattgta ttattctgaa tatagaaaga
      121 tagttactgt cttagataaa aactgaaaca tacataataa tataaaattt gctatattaa
      181 taactctaat tattttggaa ggagggaaaa tatgataagt gggagaatgt tactcatcat
      241 gaattttcta aaaggaaaaa ataagacaag ttacaaggat attagcaaac aacttagtat
      301 agaagaaaga aaagttagat atgatataaa taatttaaat attgttttgc gttcattaaa
      361 caagtcagta atacaaaaga tgaacaaagg tgtgttaatt attcctgatg acttccaagt
      421 agtatgtata aataataacg aatatgtatt ttccttaact gagcgagtat ctattatgaa
      481 aattatatca atgtttaaaa tagataatct aaacttagaa aaattaagta aagaatttca
      541 ggtttctaga agttcaatta aaaatgacct taatgttttg tcaagtgaat tagaaagaaa
      601 ccaaataact attacttatg ataaatcttt taaaataagt ggagaagaag acattatata
      661 taagcaaaga ttgaacatat taaaatcgta ttcttatctt tttggtaagg aaaaatctga
      721 tttaagtgta tttgagaagt atttagtgaa tgtaattgaa aaaatattca aagggattta
      781 tttgattgat atatataatt ggtctatgaa tttattattg caaatgggat ggactttaaa
      841 tgatgaatct tttacatggt atgtgtcaaa cattttcctt tttacatggt atatacattc
      901 agatgaaaaa catcctttag aatctgcttc attaagtgag ccgtgttttg aaaataagtt
      961 tgaccaagaa tttgaaacaa ttattcaaaa aaagttgact aaggaacaaa tttttctgtt
     1021 aaagagtttt gtatttttta caaataaata tgcaagttta aacgaggata tggatttgat
     1081 tagtacagaa attatagttc aagatctaat ttacaatatg tcagaaatat tagagattag
     1141 ctttaaagaa gatatgattc tttataaagg attactcaat catattgccc cattgataga
     1201 gagaatcaaa cagaatatac agatttatga ggaattactt catgtaatac cagataaata
     1261 tcaatatgta cttgaagcta ctaaggtttc tattcaaaaa aacagagttt taagtcaaat
     1321 aaagaatgaa aatgaaacaa tacttttggc aatacatttc ttaggaagta tacatagaaa
     1381 tgagaggaat aactacaaaa atatattgtt agtttgtgga tttggatatg gtgtaattgc
     1441 tatgttaaag gatagtttag taaatgatta ccagataaat atagtagaga gtattccatc
     1501 atataagtta caagattata aagaatggga aagtattgac ttcattataa caacatcaaa
     1561 gatagtaata gacttgccta aaacaattat tgagataaat ccatttttac aagaagagga
     1621 ttatttgaag tttgaagaaa aagggataaa aagaaagaac gtcttaacta attatatgtc
     1681 aataaaaaaa aggttggact ttttagaaga agatgcccaa aaaagagtat taagtattat
     1741 atgtcaagaa cttggataca gtgatgagag aatattattt agacaactta atttaagtga
     1801 tttaatagga attgatacca taaggataat tgatggagat atgaaatggg aagatgctgt
     1861 aaaaattagt tcaaacatac ttgctaattg tgattttgta tcagcacaat atgctgagaa
     1921 tattatagat attttaaaaa atgtgggttt ttatgctgta aaagataacg aatttgcttt
     1981 actacatggc aatgatagtt cattagtcaa agtaagttcg ataagtttat taatatgtaa
     2041 agaagcagtt agttttggag ataaaaaggt taaaatcata ttttgcctag caagcagaga
     2101 taaaaaagag caaattcctg caataattaa tctaactaaa atggtttata agactaattt
     2161 tattgctatg ttagaaagtg caaagacaaa agaagaagca gaagttttaa ttcataaata
     2221 tgagaaggag gtcactgtat gaataaatta gttaatgtaa aatgttcatt taaaatacat
     2281 gttaattcaa aagaagaagc cattatatcg ttagttgatg tagtgtcaaa agaaggatat
     2341 ttaatagata aaaatcaatt tttaaaagat gttttaaaga gagaagaaac attgtcgact
     2401 tatattggac atggtatagg tcttcctcat agtcaaagtg taggtgttaa aaactcttgt
     2461 attacaatag gaaagcttga tactcctatt gaatggacag aagaaggaga aaaagtggat
     2521 ttaatatttt tgatatcagt aacaaaagac aatgaaaata atcttcattt aaagatttta
     2581 tcaaaattag cacgtttatt gatgcatgaa agttttagaa atcaaattag atgttcagat
     2641 gaacaaactg tttataatct tataaaagaa aaaatagagg aggaagatta aaatgagaat
     2701 tttaaagttt ttgcaaaaac atcttatgac agcaacatct tatatgattc catttgtggt
     2761 tgcaggagga atattatttg ctttatcagt aactttaagt ggacaagctg ctgttcctga
     2821 aacaggctgg ttggctaaac tgaatcaaat tggagctgct ggcttggctt tattcattcc
     2881 tatattaggt ggatatatag catttagtat ggcagataaa ccaggattag ctccaggtat
     2941 gataggtgca tatttagcaa aagaagtaaa tgcaggtttt ataggtggta tcatagcagg
     3001 atttatagca ggatttgttg ttttacaatt aaagaaaatt aaattagctc ctacaatgag
     3061 aactttaggt tctatattta tttatccttt agttggtaca ttgattacag gtggtataat
     3121 tgtattttta attggagaac ctatagcttc ttttatgaca tggatgacaa actggttaaa
     3181 tggaatgtct ggtgtttcta aaattccttt aggtggaatc ttaggaggaa tgatagcctc
     3241 agatatgggt ggccctataa ataaggttgc tgctactttt gcccaaactc aagttgacac
     3301 acttccatat ttaatgggtg gagttggcgt tgcaatttgt atacctccaa ttggtttagg
     3361 attagctaca ttattgtttc caaaaaaatt tagcaaggaa gaaagagatt caggtaaagc
     3421 ttcactatta atgggatgtg ttggaattac agaaggagct attccatttg caacatcaga
     3481 tccagttaga gtaatacctt gtattatggc tgggtcgatt gtaggaaata ttatggcttt
     3541 tttattagga tgtttaaatc atgcaccctg gggaggtctg atagtgttac ctgtagttga
     3601 caataggttg gggtatattg catcagtatt aactggagca gtagttgttg cggtattaat
     3661 gaaattagta aaaaaagacg tgaaagaaga tgaagaaatt gaagaagatt tagatgaatc
     3721 aattgaatta atatttgaag aattataaat ataaatggaa ggaatttgta taaaaaagta
     3781 aatataaaac tataaattta aaggagataa gaatatgaaa attgtagcag taacatcttg
     3841 tcctagtgga gtagcacata cttacatgtc ggctgaatca cttgaattat cagccaaaaa
     3901 atttggtata gaaataaagg ttgagactca aggaagctct ggaatagata atgaattgtc
     3961 acttaaagat attgatgaag caacttgtgt aatattgact aatgatgttg agattagaaa
     4021 tatggaacgt tttaaaggaa agaaggtagt aagaatgagt gtatctgaga taattaagaa
     4081 atctgatgca ttaattaaga aaataaaaga tacttttcaa taaaaattga tttattttac
     4141 atataactca aagatgtttt taaagaaatt tttattaaaa gcatctttga aaatatctta
     4201 aaaatagaat aaaaggagat atagtcatga aaaagttacc aataaagact gtcgtgcaaa
     4261 gattactaaa cttacaagaa gaggggaaaa gtgcaactct tttaggaatt ggtcctatgt
     4321 caccaaactt gttacaagca agttttgaac tagcaaaaga tgatgatttt cctttaatgt
     4381 ttatcgcaag tagaaatcaa gtagatgcag atgaactagg tggcggttat gtaaatggtt
     4441 ggaatcagga aacttttaca aaagatatta agaaggttgc cgacaaagtt ggatttgatg
     4501 gtttatatta tttatgtaga gaccatggtg ggccatggca aagagataaa gagagaaatg
     4561 accatttgcc tgttgatgaa gcaatggagt taggaaaaaa atcttattta gcggacattg
     4621 aagcaggttt tgacctattg atgattgacc ctacaaaaga cccatttgaa attggaaaag
     4681 taattccact agatgtagta ctagaaagaa ctgtagaatt aattgagtat tgtgaaaacg
     4741 agagaaaaag acttaatcta ccagacatag gttatgaggt tggtacagag gaaaccaatg
     4801 gaggtctgac ttcaactgag acgtatgaaa catttataac tagactaaag gttgaactcg
     4861 ataatagagg tttgccaatg ccaacattta tagttggtca aactggtact ttaactagaa
     4921 agacagaaca agtaggaact tttaatttta gaaatgctta tgatttagct caaatggcta
     4981 agaaatatgg agttggttta aaagagcata atggcgatta cttagatgat gtaactttat
     5041 tagaacatat accatctcaa attattgcta caaatgtagc tcctcaatat ggtactgaag
     5101 aaactagggc atatttaaaa ttagcagaag ttgagtgtaa attagaaaaa gagggcttag
     5161 ttgagaaaac ttctaatatc agacatgttc ttcttgttaa tgcaattgaa tgtggacgtt
     5221 ggagaaaatg ggtagtagga gaacaaaaaa atctaactac tgaagagata tttaaggatg
     5281 aagttttatc taatgaaata ctagatattg caggacatta tacatttaat aatgatgatg
     5341 ttaaaaaaga aatagaagtg ctttatgata atttaagtaa gaataatatt gatggtcaaa
     5401 gatttgttgt agaccatatt aaacgtcctt tgcgtgatta tgcagaatgc tataacttaa
     5461 aaggtgttac tacgaggatt ttaaataaat aataggactt atagtggtga gtattgttat
     5521 actaggtatt tatagttata attatactta ccactattaa atttaggagg actaatattt
     5581 tgattaattt aatttttgat gtagatgata ctttatataa tcaattaacg cctttttaca
     5641 ctgcatataa taaagtgttt tcttcaataa aggatatttc tattgaagat ttatatatga
     5701 gtagtagaaa gtatagtgat gaggtatttc atatgactga aaatggagaa atgccaatca
     5761 aagaaatgca tatttatagg ataatgaaag cttttgagga gttgggaaat tctattacag
     5821 aaaaagatgc acaaagtttt caagatgagt atatatatca acaatcacaa attacactaa
     5881 taccagaagt tgagaggatt ttaaattttt ctaaagaaag aaatattaat ctaggaatta
     5941 taactaatgg cccatcaaac catcaaagaa tgaagttaaa gcagttaaat attgaaaact
     6001 gggttgataa aagtaatatt tttatttcta gtgaggttgg attttctaag ccagatacta
     6061 atatttttag agtagctgag aatgttatga atttagatag agaaaataca tattatgtgg
     6121 gtgattctta tagaaatgat gtattaggtg ctaaaaaagc tggttggaag agtatatggt
     6181 tgaaccatag gggacatgag ttagaagagt tgttttataa acctgatttt gtaattttgg
     6241 aacataagga tttgatatct ttatttataa aaatatgtag ttataaaaat atgtagtaaa
     6301 aaaatgttaa taatagaatt gtataaaata attaataata actatataaa attatatgtt
     6361 tagattgtga gcaatctatt ttatgttaaa aatagattat ttagaagaag gggaaatctt
     6421 aaaaaaattt gaatatcccc tttttattat ttaattaaaa tgaaatagtt agaaatatat
     6481 tttatttagt ttagtcaata acattttggg attctttata actacatgtt aactgttgaa
     6541 taggtttatc atatggttta acccatcctt tattaatact taattctgta agtgctgtgt
     6601 gaccttctac catttgagtt aatgaagctg aatatatagc tcttagttca ggtgtactac
     6661 ttgtcaatgc agaattaaga tacatattgg cagatgcttt agcagatgaa agcatactta
     6721 acattattac tttatcgtct atataagtat tgttcttaat tagattttct atcatatttc
     6781 tcatgttatc tatacctcct cagaaattag tatttcattt tcaactataa attgttgtat
     6841 agctttaatt cttccttctg ttgctaatat actagattct gattgtcttt ttagatcttc
     6901 atctgatatt aacatattta ttgctctttg cataagtaat ccgtcagact ccatttttat
     6961 aattgctgct aaagatattt tttcagtttc tgaaagtatt ctcatttaat catctcctat
     7021 attgtaattt atgctttaat agtatgtctt tcttttcaag gaaataacca ttacactttt
     7081 ttacagtatt ttatattctc gttatatcaa aatttagtcg acttaatata ttaatataat
     7141 tttgtaggat atttatcatt aaatatctat aaaataaatt gtttgaatat tcataaagca
     7201 aaatttattt gtatgtactt cttcaagagt aaatatgagt tttttcaaaa aattaatttt
     7261 tatgataatt attaagggct atatttttct taaagttgtg attatcatta ttaatttaca
     7321 ataaaattgc cgaaaagccc tctcctttag gtgtagggat gaaaggtaag gtcttaatat
     7381 aaaaaagtta gaataatgct tgtaatctaa taccgcta
//
LOCUS       DAYATN010000164         7374 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.167, whole genome shotgun sequence.
ACCESSION   DAYATN010000164 DAYATN010000000
VERSION     DAYATN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.167"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            119..769
                     /locus_tag="ACGI8A_003329"
     CDS             119..769
                     /locus_tag="ACGI8A_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFH0181293.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(909..2285)
                     /locus_tag="ACGI8A_003330"
     CDS             complement(909..2285)
                     /locus_tag="ACGI8A_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0181294.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2301..4214)
                     /locus_tag="ACGI8A_003331"
     CDS             complement(2301..4214)
                     /locus_tag="ACGI8A_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFH0181295.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4350..4982
                     /locus_tag="ACGI8A_003332"
     CDS             4350..4982
                     /locus_tag="ACGI8A_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFH0181296.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5121..5660
                     /locus_tag="ACGI8A_003333"
     CDS             5121..5660
                     /locus_tag="ACGI8A_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFH0181297.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5836..6006)
                     /locus_tag="ACGI8A_003334"
     CDS             complement(5836..6006)
                     /locus_tag="ACGI8A_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFH0181298.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6291..7358
                     /locus_tag="ACGI8A_003335"
     CDS             6291..7358
                     /locus_tag="ACGI8A_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFH0181299.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
BASE COUNT         2799 a          972 c         1064 g         2539 t
ORIGIN      
        1 gtagaatgaa tttgtcaagt ttaatttagt tgtaatttta tcttttatta aaaataatat
       61 aaattaaaac atataaataa aatattttat acaatatatt gtaaaggagg taactttgat
      121 gaaagcagaa aaaacaagta tatatccaat tattaaaaat aatttaaaca aaatatcaat
      181 tgttgaatat aaaaagggtc aaagatttgt agcatcagat aaagacttac aagaagtgtt
      241 ctttataata gagggagttg cattagtgga gtgtactact agaggaggaa ataaatttct
      301 tgttgacatt gtacctgaaa atgaatttat aggcaaaatt agttacatat atgaacataa
      361 tttgaaatgt gatatatttg caaagaccaa tataaaatta tttcgatttg aaaaaaatgt
      421 atttgaagag ttttatctca aaccagattt tattgcacta tttcacagaa aatgtacaag
      481 aagaatatat gaattatata aaaccagaat ggtaagagaa ctattttctt gcacagaagt
      541 tattgcctat tgtatatcaa ataaacaaga aaataatgtc tgcaatataa aagctattcg
      601 taatttcagt gaaatctatt caattagtag aaaaagtaga taccattctc tgctaaagtt
      661 aactgaaaag gagatagtaa aaaaaatggg taatacatat gaaattttaa actatgatga
      721 attacataaa ttggcatttg aagtaaaaga atttttggaa gatgagtaaa aattgttact
      781 taatcaaaat aaagtataat aatttctgta aaaaatagaa atagtctcac tgaaaatcaa
      841 aaatacttta gttattttca atgagactgt ttagaaatat tgtatactta taatgtatag
      901 tataattatt aatcaatttc ctttactata ttaatagccc attttttaga taatgctcta
      961 ggaacaccaa atataaactt aaccacttct tcactataag tgagcaaaac gagtacaaat
     1021 ataggtgcac ctttaaatgc agctataaaa gttagaggaa tagaagctaa ccacatacat
     1081 cctaattcta aaaatagagc atatttagta tctcctccac ttcttaaaat accaattata
     1141 accaatgtat taaaagattt aagagccatt agtattccca ttataaagaa tatcttagtt
     1201 atatcagaag ctaagctatc agaaacacta aacattttta ataataatgg tatatttaaa
     1261 attaaaataa ttccaagtat taaaccagct gaaaatacaa gtatagaaaa ctttttagca
     1321 tattcaatgg ctcttttaat atggtcagct ccaagttcat ttccaagcat aatagatgca
     1381 ccagatgcaa tacatacagc tgtcattatg aagaaattac ctgtactggt tgctatctgg
     1441 ctagctgcta tagcagaagt accagctctt gcatatgcaa cagaatacag gacagttcca
     1501 attgcccata cagagtcatt tacaaatata ggaaagcttt ttgatgaaaa agattttata
     1561 aaaattttat caatagcttt tagatttttt agaccaaact ttaatatata gtctttttta
     1621 taaaggtaaa cataagtaat cattaaaatt aattcacata ttctagcaat aacagtagct
     1681 agagcagcac cttttacacc caatgcagga agaccgaagt taccaaatat aaatccataa
     1741 tttaaaataa catttgttac aagtgcaaat gcactacaaa tcatacctaa cttaggattt
     1801 ctaacacctc ttgaaccagt gctaaataca gtacttacag caattaatgg ataactaaat
     1861 actgcaatac taaagtaatc tatacaaagt tttaccacct cagaatcata cgaaaatatg
     1921 tgtataattg gctttggtgt tagtaaagca ggaataacaa atacaaaact taaaacaatt
     1981 gccaatacac aagtaagacc tgtgactttt cttatattac tcacatcctt ctttccataa
     2041 aactgtgaaa taaaaactcc agcacctcct gtgataccag ataaggacat attaaaaaga
     2101 aagaaaaatt ggtttgcaac tccaactgaa gctacagatg tttcccccaa actacttatc
     2161 ataattgtat ctattacatt tacagaagta gaaattaagt tctgtatgat aataggtata
     2221 cataaagata gaagtatttt ataaaatctt ttattttcgt cttttaaaga aaatagttct
     2281 tgcaaataat cctctccttt ttacataaat tcttcccact catcatatag agaggcaagg
     2341 ttattttcta aattaatttt ttcttggcta acttctttag ctttatctgg attagagtat
     2401 acttcttctt gacataaaag tatatctaat ttttcaattt caatctctaa cttttcaatt
     2461 tctttttcta tattttgtct ttttattcta tttttctttt ctatttctct ttgttctctc
     2521 tcttttcttt tttcatcttt tatttgagtt ctagtttttt cttctttttc ttctataata
     2581 ctcatctctt ggatttgcct ttttttatct atataataat cataatttcc cagatattca
     2641 gtaattccat tttcatctaa aactaaaatt ttatcaacaa cagtatttag aaagtatcta
     2701 tcatgtgata ttgtaaatat agtcccatca taattagtta atgcctcttc aaggacttcc
     2761 ttagaatcaa tatctaggtg attagtaggc tcatctaata acaataagtt tgcatttgat
     2821 agtataagtt taagtatggc aactctagct ctttctccac cactcaatgt agaaatcttc
     2881 ttaaaaactt cttcatctac aaataaaaat gcccctaaca tatttctcaa agtagtttga
     2941 gtaagatgag tattattttc ccaaatctca tcaattattg tgttatctaa attaagagtt
     3001 ttttgttcct gatggaagta ggaaacatgt acattagttc caaatttaat attgccacta
     3061 agaggttgta gttcattagt tattatttta aataatgtag atttgccaac accatttgca
     3121 cctataagtg caactttttc ccctctatat atatctaaat ccaagtcttt aaaaagtatt
     3181 ctttctccat aacccataga tacatctcta atttgaagaa catcattacc actctggaca
     3241 gaaggcgtaa attgtatacg agcttttttc ctataagctt ctggtttatc taaaacgtct
     3301 accttgtcta atgctttttc tttacttctt gcacgcttta ggtgtttttc tctaccataa
     3361 gcttttagtc tttcaattga ttcttcttgc ttttttattt ctttttgttg gtcttcaaat
     3421 ttcttaagct ctagttcttt ttctatttta gataactcaa caaatttaga gtagttacca
     3481 ttatatactt taagtttttt gttgtgaact tcaaaaattc tatttacgga ttggtctaag
     3541 aaatatctat catgagatat taacatgaca gtccctttat attgttttaa aaatacttct
     3601 aaccattcta ttgcttcaga gtctaagtgg ttagtaggtt catcaagtaa caatagagtt
     3661 ggtttcttaa gaagtaattt tcctaaaaga actctagttt tttcaccacc agaaagtatg
     3721 cttattggct tatccatatc attgtcacta aatcctaatc cctttagaac tcctttggct
     3781 tcagatttat atccatatcc atttaaatct gaaaataatt ctagtttatg tgaatattca
     3841 tccatgattt tttctataat tggagagttt gtttttgtac tttcttcaga tattttaacc
     3901 tccaattctc ttaaatagga ttccatttct atcaaatttt taaaaacttc tagtacttct
     3961 tccaaaatag tattatccga ataaaaattg gtattttgtt ctaaatatcc gatttcacaa
     4021 tctttagaag tgtaaatatc tccactatca tatccataaa ttcctgaaat tattttaaat
     4081 aaagtagttt ttccagtacc attaacaccg attataccaa ttttatcgcc ttcatttact
     4141 gtaaaactaa tattttctaa tatagaatct ataccaaaac ttttatttaa gttattacat
     4201 gacaaaacaa tcataaattt ctacctcctt gaactatcta aaattataat gttttgatat
     4261 aatataatca aggttatatg tattagtttt gaaaaagaaa tattttattg tgaatcaaac
     4321 aaaaaagagg acacttttag gaggtgaaga tgttgggaaa taaaaatata tcaatggcag
     4381 ttataagaag gctcccaaaa tatcatagat atcttggaga cttattagat agggatatac
     4441 aaagaatatc ttctaaagaa ttgagtgata taatagggtt taccgcttct caaataagac
     4501 aagatttaaa caactttggt ggatttggac aacaaggata tggttataat gtagaagctc
     4561 ttcatactga gataggtaaa attcttgggt tggatcgacc atacaacgca gttcttgtag
     4621 gagcaggtaa cttaggacaa gctatagcca attatgcagg atttagaaaa gctggattcg
     4681 agataaaagc tttatttgat gcaaatccta gaatgatagg tttaaagata agagagtttg
     4741 aagtattaga ttcagatact ttagaagact ttataaaaaa caataatata gatattgctg
     4801 tattatgtat acctaaaaat ggagcacaag aagttattaa tagagttgta aaagctggaa
     4861 tcaaaggtgt atggaatttt gcacctttag atttagaagt tccgaaaggt gttatagttg
     4921 aaaatgtaaa cttaacagaa agtttattta ccttatcgta tttaatgaaa gaaggaaagt
     4981 agacaactta aacatattaa taatgagcag ttgtgaagag tattgagaaa gtcagaagat
     5041 ttaatattaa atcctctggc tttttgaaat attaggataa aaatttaaat aatgtatgga
     5101 aataaacaat attaaggaga gtggtttttg tggaggaatt atggaaaaag gcaattgaat
     5161 ttcatggtca tgaatgtcca gggttagcta taggatttaa agcagcactt gcagcaagag
     5221 aatatttagg atgtaaacag tcaggagatg aggaaattgt atgtatttct gaaaatgatg
     5281 cttgtggaat agatggtata caagtcgtgc taagttgcac aatgggtaaa ggaaatttac
     5341 tacttaggat gactgggaaa tcagtatatc atttctttaa tagggaaaat ggaaaatcta
     5401 taagaatata tgtaaagtca aagccatttc aaatggatag agaaggatac aaaaagtact
     5461 tattagaaag tgattttgaa gaaatatttg agattacaga tactaagtta agacttcctg
     5521 aaaaagcaag aatatttaaa tctcttaaat gtgaagtgtg tggtgaattt gcatctgagc
     5581 aatatataag gattgaagaa ggaaagaaag tatgtcttga ttgctttaat aaatatgaaa
     5641 gattttatga ggagtattaa aattataaat tagattaaag ttaaaagtat tagatatgtg
     5701 ctaacagtat tttaagatga attaggtagc aacataataa agtatataaa taatattaat
     5761 tgaatcatac ataattaaaa agaggctgat tagtatttat aaaataaaat cagtctcttt
     5821 atgaataacg aattattatt ctggttgagg tgcatctaca ggacaagctc ctgcacaaga
     5881 accacagtct atacaagaac cagcatctat aacatatgcg tcgtctccag ctgatataca
     5941 gctaacaggg cattcagcct cacaagcacc acagcttata caagcgtcag taattttata
     6001 tgccattttg aaatcctccc ttttatatgt attgtaattc aataacatta taacaaatat
     6061 tattgaatat aacaattaaa ttaattctac aacaaaaact ttattatgat attcttgttt
     6121 taaagtttta caataaagtt taatctgctt atatttttat ttattttaaa gaagtttatt
     6181 ttaaaaataa aattaaattt aaaaagttat gtattgatta tatttaaaaa tgttatactc
     6241 taaaaaagta tattttattt aaaaatttat taatacaaag gagatttact atgaaaaata
     6301 aattagttta catatgtatt atgagtctat tgctatttgt agtaggatgt tctaataatc
     6361 caaataagga aagtaccatt aaaaaagact ctaagaataa ccaaagccaa gttaaagaag
     6421 aaaaaagaga acctgtgaaa aaagatataa ctataagttt cgctggtgat gtaactatgg
     6481 ggaactataa aggttcatca tattatggca gttttgacca tgaatttaaa agacaaggag
     6541 aagattacaa ttattttctt aaaaatgtaa aacctgtatt tgaaaaagat gacttaacag
     6601 tggttaattt agaaggtcca cttactacag cgtcaaatgc aaaagaaaaa aaatttgcat
     6661 ttaaaggaaa gcctggatat gtaaatattt taaaatctgg aagtgtagag gcagtaagta
     6721 ttgcaaataa tcattcagaa gattattttg aagaaggaat gaaagataca cagtttatat
     6781 tggacgagaa taaaataaat tattttggat tagggaaaga tgctgttgta gatgtcaaag
     6841 gaattaaagt gggtctatta gggtataatg gattatcaac tgaatataat gaaaaaaatt
     6901 taaaacaaat ggaagatgat attaagtcct taaaaaagaa atcggatgtt gttgtagtgt
     6961 actttcattg ggggatagaa ttagattatt atccagataa aaaacaaaaa gattttgctc
     7021 attatgctat agacaaggga gcagatttag ttatggggtc tcacccacat gtgattcaag
     7081 gaatagaaaa gtataaaaat aagtatatag cttatagtct agggaatttt tgctttggag
     7141 gaaataagaa tccttctgat acagatagtt atatatatca acaaaccttt actttttcag
     7201 ataataaatt aacttcaatt aaagagccaa atataatacc aacttcaata acatctagta
     7261 actccagaaa taattatcaa ccaaaaatat tggatggaag tgagaaggat agagttttaa
     7321 ataaacttga aaaaataagc aaagatttaa ataaataaaa tgtaaaaaaa aata
//
LOCUS       DAYATN010000165         7373 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.168, whole genome shotgun sequence.
ACCESSION   DAYATN010000165 DAYATN010000000
VERSION     DAYATN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.168"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            195..377
                     /locus_tag="ACGI8A_003336"
     CDS             195..377
                     /locus_tag="ACGI8A_003336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181300.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            1204..2118
                     /locus_tag="ACGI8A_003337"
     CDS             1204..2118
                     /locus_tag="ACGI8A_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HFH0181301.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2508..3155
                     /locus_tag="ACGI8A_003338"
     CDS             2508..3155
                     /locus_tag="ACGI8A_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HFH0181302.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3322..3891)
                     /locus_tag="ACGI8A_003339"
     CDS             complement(3322..3891)
                     /locus_tag="ACGI8A_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181303.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(3896..4795)
                     /locus_tag="ACGI8A_003340"
     CDS             complement(3896..4795)
                     /locus_tag="ACGI8A_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0181304.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIIFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4806..4955)
                     /locus_tag="ACGI8A_003341"
     CDS             complement(4806..4955)
                     /locus_tag="ACGI8A_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HFH0181305.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(4948..5532)
                     /locus_tag="ACGI8A_003342"
     CDS             complement(4948..5532)
                     /locus_tag="ACGI8A_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HFH0181306.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            5921..7246
                     /gene="cdeA"
                     /locus_tag="ACGI8A_003343"
     CDS             5921..7246
                     /gene="cdeA"
                     /locus_tag="ACGI8A_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HFH0181307.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIFALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
BASE COUNT         2636 a         1052 c          897 g         2788 t
ORIGIN      
        1 aattaaactt taaactggat aaattttctt ttaagaatga aagtttatcc agttttgcga
       61 ttataataac cagttttaca tatccacttg taaaatatat ctaagcaata tatcatacta
      121 tatagattta tgagtgaaat aatatctgct atttactgaa tatttttatt tttcgttttt
      181 tagggggatt agttgtgttt aaagataaga ttgatgaatg tgttcatata atgactgcat
      241 atattgctag tttaaaagaa tactattcat tcatagaaac tcaaattggt gattttatta
      301 aaaaatatgg agaagatgtt gtagagttat gtctccatag agtgatgatt ctactatgcg
      361 aatgtggttt ggcataaaat taaatttatt aaaatgggta agaagaatcc tatataatat
      421 tcttcttacc cattttaata tatcaatttc atagcttatc gtttaaaata ttttacatcc
      481 attactagac aactttacat tctcttttta attctagtat atttaaatca ttttagttgc
      541 tccttattta ttcttattac ttgttttatt tataaaataa ttctttttaa taatgttttt
      601 tgttgatatt cctatcacac atccaattat ttatcattgt cttgaaattg tttgcatact
      661 taatttaatc caattgaaac accccagcat agaatcccct gtaaaataga agttggatta
      721 aatcctaaca tctatacaga aaatactact ccaagcacaa gcaatataat ggaatatatt
      781 tatcacttaa ttttttatac tttttaaatc ccatacctaa aacattaagt gcagctatta
      841 gaattaataa ttgttgtggt ataaaattat gcttgcttca tatttattca ttttttgcta
      901 taaaagttct tgttttacaa aaaacatttg ttttgtatca aaataatact tataaattac
      961 ataattctca taaaaatttc tagtattact tgtactctta ataaataata gccaacaagt
     1021 accttaaatc attatatcta tatctaataa gaaacacagt aagataatct tttcgcccat
     1081 aacttaataa aaactgttga tattagtagc aattgaattt agttaattta tttataatat
     1141 acataaattt aaatattacg aataaagtat tataaatagg atactttcta aggggaggaa
     1201 attatgatag ataatcaaaa atatgttatt ttatcactag aattacattt atttttttca
     1261 agaattatga aagagcatgc tcttttttta gaagcaggat tcacaaataa aaattataat
     1321 cttgctatgg aagctgacca ctataaaaag caatttgaag atttattatc atacactgtt
     1381 agtgctagta atggtataat tagacctgat atattatatt cagaagagct tgtaactact
     1441 ctcacatcag ttgcagaaca aaaaacagaa gagtttacag ggatagaaat aaacaaaaac
     1501 atcactacaa gagaattaaa tctacaaagt ggtgtaaacc cacaagttgg tcaagactta
     1561 gtgaactatg tagctcaact taactctgat gcaataagat tacttgatgg gcttattaat
     1621 tttaaagaaa gagtcttaga tggtgtacta tcatgtacta tatttacatc aaactaccct
     1681 ctacttcttg aacatataat acatgaagca aatttatatc gttcttatgt agttgacctt
     1741 gaaaataaaa tagatattga gtcaaaaaac gctaaagaaa tagaattatt ctgggaccat
     1801 attatgatgg aacatgctct gtttatgaga ggattactag acccctcaga aggtgaacta
     1861 ataaatactt caaatgattt tgctataaaa tttaatgaat taattgaaaa aacaaacgaa
     1921 atgactgatt ctaatatcaa gaacattaca gaagaaactc taaatgaaac tgttgagttt
     1981 aaagatttta aagaagcagg agcatcagga atagaacagt gtaagataaa atctataata
     2041 ttaccacttt tagcagacca tgttttaaga gaggcaaatc attatattag aatattggag
     2101 agttataaaa acatgtaatt aaaaattact tttaaaataa tctttgcata tttagataaa
     2161 ataaaggtac agttaactgt acctttattt tttataaact tataaaattt aaacaagtta
     2221 ttcataaaat aaaataataa tcatctataa cccacaactt tattttatat taaataaaaa
     2281 gtattagtgg ttatgtttta taagtatttc tttattcagt tatttattaa aaactaatag
     2341 taaatcttat actaatcgca tatttatgtt ataactatat tgtaactatt ttcgtaatct
     2401 taggaaaaga ttacttataa aattaacctt atacaataat atatcgtata atcttaatta
     2461 gcataatagt tacaatatta aaatttaaat atgggagtga ttttagtatg ataagacaaa
     2521 caccattaga gaaacgatat gacaagctag gtcctcatat tgtaacagca ctcaaaaaac
     2581 gttattttga tgcttattac tgtaaaacta gaaatgaagc tttgcaacaa attttagatt
     2641 taattcctca aaatcatgta gtatcatggg gaggatctga aacacttaaa gaaatgggcg
     2701 tacaaactgc tgttagagag aaaggattta aagttattga tagagattta gcaaaaactc
     2761 cagaagaaag aactgaaatt atgcgtcaag cacttctatg cgatactttt ttgatgagta
     2821 gcaatgcaat tagtgaagat ggacaactct ttaatattga tggaaatggc aatcgtgtag
     2881 ctgcaatgat atttggtcca aaaaatgtta tagttgtagc tggtatgaat aaaattgtaa
     2941 aaaacattga tgatgcaata caaagaacta gaacaattgc atctccagca gttgttcagc
     3001 gttttcaaga tgtcaataca ccatgctata ttaatggaag ttgtatagat tgcacaagtc
     3061 ctgaaagcat ttgtgcatac atggttacta caagaatcag tcgtccagca aaaaaaataa
     3121 aggtaatatt ggtaggagaa aatcttggtc tataaaatat acatacatgt aaatagctct
     3181 ataaataata aaatcatatg aaataactta tttctaaaag tggtagaata aaagcttcta
     3241 caagactttc attctgccac ttttatattc ttttaatttg tgtttctata ttattaattg
     3301 ctccaatttt catatatatt actaactatt tttattttca taattttttt gttcttcatc
     3361 aagctgttta taataatctt tattatagtc agctagataa tctataccac ctcctgtaat
     3421 cacatttaat aatttcattg cagattcttg attaacttta caaacataat tatcaccaga
     3481 tttaacaagc tctaatgtaa tattaaattc ttttttcttt gcagaatcaa tatctttcat
     3541 agttggattt ggcatcattt ctcctagttt aggaggattc ttttttaaaa tagaaaccat
     3601 atcagtattg ttaattttta aatctacagt cactttatcc ttattatttt ttatatctga
     3661 aatttcccaa gtcatatttt tagaatacat tttaaatcct tctttatctt cgtatataag
     3721 attacctaac cctgattttg ggaaatactt atcaacagta tctgcatctt gtgtttttat
     3781 tgaattaaac atactatcca cttcattttt tatcttatta tcattagtac aaccagctaa
     3841 caccagtact gatattgcaa acattataat acatagaata aatttcttca ttaaattata
     3901 tccccctata cttttttgca caaatattat tctctttttt atcttttgaa aaagttgtag
     3961 taattgcatt ttgtggacaa acttttatac attcaccaca cctaatacag tctggactat
     4021 ttacatcttt atatattggt atatcaagct tacattttgt ttgacatgct gtacaatttg
     4081 tacatttatc tttatcaatt ttaaatctat atattgaaat aggattaaaa aaaccataga
     4141 ttgctcctaa agggcatata tacttgcaaa atggcctata agtaatgatt gacatcaata
     4201 atatcaataa caaaatactt aacttccagc taaataaaaa tcctattgat tctcttaaat
     4261 tctcatttcc taaaataagt ggtattcctc ccaaaagtgt tcctgaaggg caaatccatt
     4321 tacaaaacca aggatttcca ccccctgcta catttacaat agcaactggc aaaataatta
     4381 caaatattat taagactata tactttagat attttagata tttatcacca taaacttttt
     4441 ttatctttac tggaaagggt actttatgta gtaaatcttg tacaagtcca aagggacaaa
     4501 gccaaccaca aacaaatctg ccaaaaaaag caccaaatgc cattaaaaaa ccaacaacat
     4561 aaaatgagaa tttatactct ctgcttgata aaactgcttg taatgaacct attggacatg
     4621 ctccaagtgc acctggacat gaataacaat tcaatccagg tacacataat tttttacttt
     4681 ctcctccata tatagttcct tctaaaaatc catttacata tccatttgtt aatgctgtaa
     4741 atgcaacttg agtaataagt cttatattat tatttacttt agataataac ttcattcctc
     4801 ttctcctaac caataccaat acactcaaga caaatcataa ttgctttagt taatactgta
     4861 tttgtttctc cacgatatgt acctaatgca ataaatataa tactaattgc aataatagaa
     4921 atgccttttt tattatcttt aataaagtta ctcattggct tttaccattt ttagtctttc
     4981 atctataatt tttttccaat cttccttact atttgcacct acaaccttct cacccacaaa
     5041 gtttcctttc ttgtctacaa aataagttgt tggaaactcc ataactcttt taaaatcagt
     5101 ttttttaagt tcttcagata cagtcaattg ttggtaagtt gcatctgact ttttcataat
     5161 gtctttaact aaatttttct cttcatttga aagaccagtt ctcatatcag ttttatcaac
     5221 ttctacaact aatcccttta tagctacatt ggaattatta ctctcatatt cttttgataa
     5281 tgcttcaagc tctggcattt cacccacaca tggtccacac caagtagtcc agacattaat
     5341 aagagtaagt tctttatttg aaaatatctc ttttgttacc ttatttccat caacatcctc
     5401 cgtatctaaa ttactaaacc attcactatt attcttgttt ttttctactg gcttagattc
     5461 ttttttgcta catccagaaa tcatacctaa aacacaaata catcctaaaa ctactatgaa
     5521 aaaccttttc atatctcaca ctcctagaat ttatatagta tataaaatcg ctgcttgaaa
     5581 taaaaataac ttatcttcta tattaaagta attatattta ataaataaga gctaatttaa
     5641 aaaaagatat acagttaaaa tctatttctt ttcagacaaa atcttatata tatataatag
     5701 cttgtaaatc ttaagattat atacatatat ttctaaagat tttcttaaga aacagttaga
     5761 ttttactaat ataagtttat taattagcat tatactaatt tatcttaata tgattttttg
     5821 ttgattttaa gctgtttatc gtataaataa aatttttctt gactatatag ttactatata
     5881 gcttattata tatagagagt aataaaagga ggtaaaattt atggaaaatt tatttacaag
     5941 aaaattcact acttttgaat ttctaaaatt tgtttctcct gcaattatat ccatgatatt
     6001 tatatctttg tacacaataa tagatggcat ctttgtatcg acattagttg gttctgatgc
     6061 tcttgctagt ataaatattg tactacctat aattaacctt gtttgtggat ttggaataat
     6121 gatggcaact ggtggaggtg ctatcgtttc tatacgtatg ggtgaaaata gacaggatga
     6181 agccaactct acattttctt ttatagtttt gttttcattg attgttggga ttttattcac
     6241 agtaatctca tatttcttta tcaaagaaat atctattttg cttggtgcaa cagataagtt
     6301 attaccatat tgtataactt atggtaaagt tatgatttta tgtacaccat tttatatttt
     6361 aaaatttata tttgagtact ttgcaagaac tgatggaaat tctaaattta gtttatttct
     6421 atcagtcatt ggtggtgtaa caaatataat attggattat gtatttatta aatattttgg
     6481 aatgggtctt ttaggagctg cagttgcaac tgctataggt attattttaa cttgtgtttt
     6541 aggtattatt tacttcttat ctaataaatc tacactaaaa ttaagaaaac caaaaaccga
     6601 ttttagactt ataagagata ctatgataaa tggttcttct gaaatggtta cagaattatc
     6661 tacaggaatt acaacattct tatttaatgt agtagcttta aaattagcag gagaaaatgg
     6721 acttgctgct cttactatag tattgtatgc tcatttttta atgacatcag tctatctagg
     6781 attcgctgct ggagtgtctc cattaataag ctataatttt ggtgctgaaa acagtgataa
     6841 attaaaagaa acatttaaac attctctaaa atttatattt gtttcttctc ttttagtgtt
     6901 tatttttgct ttagtatttg caccatttat tgttagggtc tttgtaagtc cagataacac
     6961 agtatttaaa ctagccttac aaggattaaa aatatttgca tttgcttttt tgtttgttgg
     7021 tataaatata tttgcatcag gattttttac agcatttcac aatggaaaaa tttcagctat
     7081 tatatctttt agtcgtgcct ttgtttttat aatcatagga atcataattc ttcctcctat
     7141 gttaaacatg actggattat ggcttacagt tccatttgct gaggttataa ccatatttat
     7201 atctattcta tttataaaaa aatataaatg tagatataag tattaaaaat taatacttaa
     7261 aatcaacatt tattaaaact aataaacctg ctttaaaata caaatttaca aatttgattt
     7321 ttaagcaggt tttattttag ttataataag tcatttgcat actctttaat tgg
//
LOCUS       DAYATN010000166         7200 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.169, whole genome shotgun sequence.
ACCESSION   DAYATN010000166 DAYATN010000000
VERSION     DAYATN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.169"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            99..542
                     /locus_tag="ACGI8A_003344"
     CDS             99..542
                     /locus_tag="ACGI8A_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0181308.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            573..2390
                     /gene="ftsH"
                     /locus_tag="ACGI8A_003345"
     CDS             573..2390
                     /gene="ftsH"
                     /locus_tag="ACGI8A_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFH0181309.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            2568..5270
                     /locus_tag="ACGI8A_003346"
     CDS             2568..5270
                     /locus_tag="ACGI8A_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0181310.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            5304..6002
                     /locus_tag="ACGI8A_003347"
     CDS             5304..6002
                     /locus_tag="ACGI8A_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0181311.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            6359..>7200
                     /locus_tag="ACGI8A_003348"
     CDS             6359..>7200
                     /locus_tag="ACGI8A_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HFH0181312.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGM"
BASE COUNT         2862 a          775 c         1291 g         2272 t
ORIGIN      
        1 aatatttaat acattaaaca ttatagtgtt aagtatttat ttacagtatg cacttcagcc
       61 taaatttgaa ataaagtatt ttcgtaggga gcgtttgtat ggatgagatg agttttaaga
      121 aagagctact agagttgact agagatataa atatgaaatt tacaacttta ttaagtgatt
      181 tttatcagcc acttggtatt acagcagtgc aagctttgat tttatctgag ttatgtgaac
      241 atggagagaa aaaaataagc gatttaggga agaacttaaa catgacaaat agtaatgtat
      301 cagtaatctg tcaaaggctt gaaaaaaatg gctttttaaa tcgcataaga gatatagaag
      361 accaacgaat tgtaaaagta aaagttacaa ataaatcatt ggatatacaa gaacatattt
      421 caagtagtat ttttgatagt tattttgaaa atatgaccag tgaaaaattg caagatatgg
      481 aagatataat agaaggttta gagaaattaa ataaactttt aacttacatt gattgcaaat
      541 aacataaaaa taatttaaag gagaataaaa ttatggatga acataaaccc caaaaaaggt
      601 cagcactcta ttattataca gttataataa tagtagtaat gtttttaaat gctttaatat
      661 ttccaagttt aatggataaa aaaatggttg agattgacta tggaaagttt ctcacaatgt
      721 tggatacagg aaaggttaaa tctgttgaga tagaaaataa tagaatagca ataaatccat
      781 ctagtagcaa ggataagaat atatacataa ctggtagaat ggatgaccca gaattggtag
      841 atagactaaa aaatgcaaaa gtagaattta ctaaggttat tcctaaggaa aattcacctc
      901 ttttaagtat tttacttaca tggataatac ctataggtat tcttatgcta tttggtaact
      961 taatgatgaa gtctatgcaa aagcgaatgg gtggtaatgc tatgcaattt ggaaaaagca
     1021 acgccaaaat gtatgtatct gctcaaagtg gaaaaagatt taatgatgta gctggtcaag
     1081 atgaggcaaa agaagcttta actgaaatag tagatttctt acacaatcca gaaaagtata
     1141 aaaagatagg agcacaaatg cctaaaggag cacttttagt aggacctcca ggtacaggaa
     1201 aaacactcct tgcaaaagct gtagcaggtg aagcaaatgt accatttttc tcaatatctg
     1261 gttctgagtt tgttgagatg tttgtaggaa tgggagcttc taggattcgt gatttattta
     1321 aacaagcaaa agaaaaagct ccatgtatag tgtttataga tgagattgat actattggta
     1381 aaaagcgtga taatggaagt ggattaggtg gaaatgatga acgtgaacaa actttaaatc
     1441 agttgttaag tgagatggat ggttttgatg gtggaattgg agtagtaata cttgctgcta
     1501 caaatagacc tgacagtctt gacaaagcat tacttcgtcc aggaagattt gatagacgta
     1561 taccagttga acttccagac cttggaggac gtgaagctat attaaaggta catgctcaaa
     1621 atgtaaatgt aagctcagat atagattata atgcaattgc aaggtctaca tcaggagcat
     1681 caggtgcaga acttgcaaat attgtaaacg aagcagcact tttagcagtt aagtctggca
     1741 gagatatagt agaacaacag gattttgatg aatctgttga aactgtaatt gctgggtatc
     1801 aacgtaaagg agctgtaatt tcagataagg aaaagaaaat tatagcatat catgaaatag
     1861 gtcatgcact tgttgctgct atgcaaaaac attctgcacc tgtccataag attactataa
     1921 tacctagaac ttcaggagca ttgggatata ctatgcaagt tgctgaagat gagagtgtac
     1981 ttatgtctaa agaagaagca attgataaaa taactacttt tacaggtggt cgtgctgctg
     2041 aagaggttat ctttaacact tgtacatcag gagcatcaaa tgatatagaa caagctacta
     2101 aaattgcaag agctatggtt actcgccttg gtatgagtga agagtttgat atggtcgctc
     2161 ttgaaactgt aagcaatcaa tatttaggag gagatacttc tcttgcttgt tcttccgaca
     2221 ctgctgctaa aatagatgaa gaggtattta aaatagtaaa ggcttgtcac aaaaatgcaa
     2281 ttgaaatctt agaaaataat attgataaat tgcatgaact tgctaattat ttattagaaa
     2341 aagaaactat tacaggtgat gagtttatgg aaatacttaa cttaaactaa aatgattata
     2401 cacaagataa aaatgcctat tgttaggcat ttttatttta caactaaatt ttttaaattt
     2461 atatttaaat ctttacataa tcttaatcat taaaaataca tcttaactac aattttatat
     2521 cggaattgat attataatag taatgaatgg tgtaaggagg ttttttaatg gatagaccta
     2581 atcctgatat tctattagaa aaaattaaaa atgaagaaga aaaactaagt agaggtcaat
     2641 taaaaatatt ctttggttat gcagctggag taggaaaaac ctactctatg ttggaatctg
     2701 cacaaaattt aaaaaaagtg ggtgtagatg tagtagttgg atatattgaa ccacacacta
     2761 gaccagaaac attggcttta cttgatggct tggaaattct tcctgtaaag gagatagaat
     2821 ataaatctat aaaactaaaa gaatttgatt tagatgctgc tttagcaaga aaaccagaag
     2881 ttatattagt agatgaattt gcccattcta atgttgctgg gttaaggcat acaaaaagat
     2941 ggcaagatat agaagaattg ctgttagcag gcataaatgt ctacactact gtaaatgtac
     3001 aacatttaga aagtctaaat gacattgttg agataattac aaacgtatca gttaaagaga
     3061 ctataccaga taagtttttg gatacaaata cacagttaga acttatagat gttgaacctg
     3121 atgtacttct tgaaaggttc aatgaaggta agatatataa gaaagaacaa gctactagag
     3181 caaaaaataa tttttttata aaagataacc tggttgcact tagagaaatt gcacttagaa
     3241 aaacagcaga gagagttaat aaagaagttc aaatgaccag actatcaaag ggtgatgtaa
     3301 ctgtaatacc tacatcagat acacttcttg catgtataag tccatctcca tcttcagcaa
     3361 aagttattag aactgcatcc agaatatcag acagtacatt tgcaaaatgg atagctttat
     3421 atgttgaaac tccaaacaca gcaagacttc catttgatga acaaaaacaa ctacaaaata
     3481 acctaaaatt agctaagaaa ttaggtggag aaataattgt tttacatggt gaaaatataa
     3541 tagagcaaat acttagaatt gcaaagctga gaaatgtaac aaaaattgta ataggtagaa
     3601 atcattcaag taataaaaga tttagtaaaa gatttaaaaa agatatagtt gacaaattaa
     3661 ttgatgaagt tgattatata gatatacata taattccata taaatttgcc tcagatgtta
     3721 aatacaaacc taaaaaggat aaatcatcaa taaaatctaa atttaaaatt agcaaagttg
     3781 attttataaa acttatattt attactttga tgataagtat tttagcatat gcagtacaaa
     3841 gcatgggatt tataagagaa aatatattgt taatatatat gttgggtgta gttttagttt
     3901 ctatgtggac taaagggtat agtacaggta taataagttc tgtatttaat atagttcttt
     3961 taaattattt ttttacagca ccattatata cattatctat agcagattca aactatatag
     4021 taacactggt tgtattctca atagtaggta taataactag cactcttacg tctaagatac
     4081 agcatgaagc agaaactgct gctaaaagag aagaaaatac aaaaatgata tatcaaataa
     4141 ttaagggatt tttaagatta tcaaataaag atgatatagt aaacaaaggt atagaacttt
     4201 tgtctcttag cttaagtaga gatgtaattt gttatttaaa tgtagataag cataataaat
     4261 caaagcttta caaaaaaaat actaataata aggataaaaa tgatttggat agtgaagatg
     4321 aaaaagctgt tgcaagttgg gttttatcca attcaacagt agcaggaaat gacacggata
     4381 cacttcctgg ttcaaaggga tactatatac caattgtagg aatgaattta actttaggag
     4441 tagtgggaat atcatgtatt gattcaaaat tggatacaga agatataagc ttaattgaaa
     4501 caataattgc tcaaatggca atagcgcttg atagagaaat cttgtcagaa gctaaagaaa
     4561 ataccaatct tgagattgaa agggaaagat taagaagtaa tcttttaaga gcagtttcgc
     4621 atgaccttag aactccacta gcaggaatat ctggtgcagt aagtacaatt ataaaaaaca
     4681 agggtactat aggtcaagat ataatagatg aattacttag tggtgtatat gaagatactc
     4741 agtggttaat aagacttgtt gaaaatttac ttagtatgac caagatagat gaagggaaat
     4801 tggaagttaa aaaacattct gaactggttg aggaaattat atcagaagcg ttacaaaaaa
     4861 taaaaaaaag aattgaaaat gcacttatag atattaatat accagaacag atattatttg
     4921 ttcctatgga tgcaaaatta atagaacaag ttttaattaa tttaatagat aattctttga
     4981 aatattcaaa ggaagattgt aagatagaga taaatgtata tgagaaagat gattatgttt
     5041 ggtttgaagt atctgataat ggtcctggta tatcaaagga acttaaaaag catatctttg
     5101 acagattttt tactggagaa gaaggagcta aagattccag aaagggtgtt ggacttggat
     5161 tatcaatttg taaatctata attcaagctc ataaaggtga aattatggta gaaaacaata
     5221 aagataaggg ttcaactttt aagtttagtt tgcctaagga aaatgaatag ttttaaattt
     5281 taaaattagt tttggaggaa gaaatgagta aaaaacttat attgttagta gaggatgata
     5341 agacaataag aaaatttata tctactgcac ttttaacaca ggattatgat gtcaaggagg
     5401 ctataactgg aaaagaaggt atatctatag ctgtctcata ttctccagat gttgttcttt
     5461 tagatttagg cttagaagat atggatggaa tagaggtaat aaaagctatc agacaatttt
     5521 caaatatacc aataattgta gtttcagcta gagagcaaga tagagacaag gtagaagtat
     5581 ttgatgcagg ggcagatgat tacttaacaa aaccattctc catagtagag ttattagcaa
     5641 gagtaagagt agcatttaga cattcacaag tagaagctca acaaaaagat gatgttaaat
     5701 ctacgtttga agtagataaa ttattgattg attttgataa aagaaaagtt attgttgatg
     5761 atgttgaagt ccatttgaca cctattgaat ataatattct ttcattactt gcaaagtatc
     5821 atggaaaagt gcttactcat aattttataa taaaagaaat atggggtagt gttataggga
     5881 atgagactaa gtctttaagg gtctttatgg caacgttaag acgaaaaata gaaaaacaac
     5941 ctgcaaaccc aagatatata attacagaag ttggtgtagg atacagatta aatgatgaat
     6001 agttgtaata tattaattat tagattatgt gcttattaat atatattaaa attatatagt
     6061 ttagttaaaa taagaatgaa gtgtatgtgc ttgttgtcag tttagcacat gcattttatt
     6121 ttttagcttt tttagtatca ataatttttt agtatcaata atatgaaata tgttttaaaa
     6181 taagtatata gtgtaatatg taataaaaac tttggttaaa taataaatgt atttaacact
     6241 taccttataa taaatttaat ataaatatta aataataatt attaaaatag tgtaaaataa
     6301 ttacagtaag gattataaaa aatttttaga atgtaaattg aaatttagga gtagatgtat
     6361 gtatatactg aatttaaata caagagagac tattgaagat tttagagata aattttatgt
     6421 agcagaaaat tcttatctta tattaagtgc tccaaaaaat ttaaaattgc ttaaggaaac
     6481 tttggatata gatgaaataa cttttaatga ttgtcttaaa tttgatgaaa taaccaaact
     6541 tgatttattt gataattacg attttttaag cctaaataca tttgaactaa gagatggaga
     6601 agctgttata gaagaggtaa atatgtattt atcagataat tttatattgg ttgtagtgaa
     6661 tgaagaacac tttttatttg aatttgtaaa aaatattatt ttgaagaact ctcaacttga
     6721 aaaaaatcca gttattaatc tatttaagat aaactacttg attcttagag aagttataaa
     6781 aaatggtttt gagtctttgg aaaaagttga agagttaatt cttcaaatag aagatgaaat
     6841 gatggacaac attaataaga atcatgtatc tagaataagt gatgtaagag gattaacaag
     6901 aattatagtt aagaatacta gacctctttt gtatattggg gatagaatag ttaaggaaaa
     6961 tataagatat ttaaaatatt caaatgtaaa aaaatataat ttagaaaatt ttcaaggtat
     7021 agattttgga attgacaaat tatacaattt cgcattgtca acacgagagt tagcagataa
     7081 attattagat atttattctt ccagagtagg tgagaaaaca aataacttaa ttacaaaatt
     7141 aaccttgtta acagccatat cagcaccact tacaattata actggtatat atggaatgaa
//
LOCUS       DAYATN010000167         7190 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.170, whole genome shotgun sequence.
ACCESSION   DAYATN010000167 DAYATN010000000
VERSION     DAYATN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.170"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            292..1818
                     /locus_tag="ACGI8A_003349"
     CDS             292..1818
                     /locus_tag="ACGI8A_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0181313.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1846..2403
                     /locus_tag="ACGI8A_003350"
     CDS             1846..2403
                     /locus_tag="ACGI8A_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181314.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2925..3962
                     /locus_tag="ACGI8A_003351"
     CDS             2925..3962
                     /locus_tag="ACGI8A_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181315.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            4073..5215
                     /locus_tag="ACGI8A_003352"
     CDS             4073..5215
                     /locus_tag="ACGI8A_003352"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFH0181316.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            5669..7060
                     /locus_tag="ACGI8A_003353"
     CDS             5669..7060
                     /locus_tag="ACGI8A_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFH0181317.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
BASE COUNT         2925 a          635 c         1271 g         2359 t
ORIGIN      
        1 actgattgca taaaaatgat ttttatataa aatttaagca gaagaataaa tttctaacga
       61 aattggatat ttcttctgta aatacatatt caaaaaaaga attataagag tttagtcagc
      121 agtcgtgtaa gtaataagga ctttataaat atatagaaat ataatgatat taatataacc
      181 aagcgatttt taatttagct ttttaataag cttatttttt atactattta taataaataa
      241 actccatgat gggaaaacta ttactattta atatgtaaag gagtatttta aatgaatgaa
      301 tcatattggt ttttaaactc aagtccaaaa gaatataaca aattaggtga aaatataaaa
      361 acagattgtc ttatagtagg tggtggaata acgggattga caactgcata tttattagca
      421 aaagaaggta aaaaagtagt gcttgttgag gctgataaaa taggatatgg aacttctgga
      481 aggaatactg gtaaggtaac ttgtcagcat gatatatttt actctaagat agagaaaaaa
      541 tatggattag ataaagcaaa atcatattat aatgcaaata atgaagcttt aaatttagtt
      601 gaacaaataa tagaagaaaa caatataaaa tgtgatttta aaagggaaac ttcttttgta
      661 tttacagaaa aagaggatac tataaaggat attaaagatg aatatcgtac atgtaaaaaa
      721 ataggaataa attgtgagta tcatgaaact atagaaaata ttccattaga tataaagggg
      781 gctattagtt ttactaatca aggtcaattt aacccaaaga agtatattga tggattggca
      841 aaagcagcag taaatttagg gctaaaaata tatgaaaata caccagtagt agaccttgaa
      901 aaaggaaaaa tatgtagggt taaaacaaga gaggataata taatagaagc tgagaatgtt
      961 ataatttcat ctcatagtcc atggtatgat ggattgaatt tttattttgc aaaagaatat
     1021 gcagagcgtg cttatttaat ggttgcagtt ttggaaaata aattagcaga tggtatgttt
     1081 ataagtatag atgacccatc aataactttt agacaatata atgatggaag tgaaaattta
     1141 ctgatttttg gaggaggaga ccataaagtt ggtcagggag gaactgagaa agaaatattt
     1201 gatgatttag agcattatgg taaagaagtc tttaaggtaa aagattttaa ggggaaatgg
     1261 tctgctcaag ataatatgag ttttgacaat gttccttata ttggttatat caacaaaaga
     1321 gaagacaata tttatgtagc tacagggttc tctaaatggg gaattactaa tggaactgct
     1381 gctggaataa taataaaaga tttaataata aataataatt ctgattataa agatactttt
     1441 aatccatcca gactaggtag ctacttttca aaagatttta taaaggaaaa tgcaaacgta
     1501 gcaataaatt atgtaagtgg aaaattaaaa ataggtagtg gagatatgcc taaaaataat
     1561 ggagaaggaa agattgttaa tattgatgga aaaagatatg gtgtatataa ggatgataat
     1621 ggagattttt atattgtaga cactacatgt actcatttag gatgtgaact taattttaac
     1681 agtgaagaaa aaacatggga ctgtccatgt catggttcta gatttgacta taagggaaat
     1741 atacttgaag gaccagctct taagccatta aaactttatg gacatggtga taatgatgtt
     1801 aatcctaagt tattataaaa ttatttttag gaaggtaaag tgtatatgaa taaactttta
     1861 aagccaataa ctatactatt gtatatagtt tatattttat ctgccgtatt ttttgtagca
     1921 aacaaagaat atggaagtct aggtctagta gcaattagtt tagttggtac tttcattttg
     1981 agttttataa ataagcaaac tgaaagatta ttgagtaaag aattatatat aatgcttgct
     2041 ttatttataa tgttttcatc attgttaggt acttgtttta atttttatga tataaattat
     2101 tatgatgatt ttttgcattt atggtcaggt ataatatctt gtattgtggc tttttctata
     2161 attaaatact tttatacaca aagagagata agtaaaatga gtaaagtgtt tttagttata
     2221 tttttattta tgtttagtat gggagtagcg agtttatggg aaataggtga atttttaatg
     2281 gatacttttg ttgggactac tacacaggcc ggagggttag aagatactat gattgatatg
     2341 gtagatgctt taataggcac aattataact attcctttta tagtaaaaaa atctaatata
     2401 tagttttaaa ttttttatat taattataat ggatttaatt aatcatgttg agtttcaagg
     2461 tctatatgat taattaaatc catttttgtt atttataatt attattgaat atatttttac
     2521 atataagata taaggtgata gatagaaatt gttggattat gtaaataatt aatatataat
     2581 accattatgg gcaataaata caaatcaaat gataatatag tctatttatg taaatgttat
     2641 attgtatcct aagtataata gtaagttttt tgatagtggt gtagaaagta aaaatctact
     2701 agcctaaatt gatgtatgga ttagtttagg aacagtaatt gcatatctta agtttgatgt
     2761 catgttgggc tactagaaat aggtcatctt tttcacttga atatcttttc tttaaacatg
     2821 gggagtatca tatcaaaaac atatgaaaat taagtgattt atgagaacag taatatttta
     2881 tatgatagta tagaatagta ataaagtatt aggaggaaca ttgagtgtta aaaaagtggt
     2941 ttggtattgt gaaaaaaaga caaaaaagtg agtcagtaaa agaagaaggt gaagtaatat
     3001 taaaaaacga aaaaatatta tctgaagaaa agttgataga tgaagaagga gttgtagtta
     3061 atattgataa tcaaatatta actaaagtag taaatgatga caatgaaata gaagaaaaaa
     3121 taatagaaga agatttgtta attagtgaaa ataccataaa attagatgat aaagaagcaa
     3181 ttattaatga taaaaacata gaattatgta aaaaagaagt tcaagttgaa agtgaaaaaa
     3241 taggtttaaa taagtttgaa ggactggacc aaaatgaaaa ttataatcta gaaaaaaatg
     3301 ttattgaaga aaaaaaagta agtgaatgtt tgacagaaga agatttagag tacataaaag
     3361 aaattaaaat aaaaagagga aaaagtataa aagctataaa cttgtatact aaagaagagt
     3421 gggtttttga cactcatata cagtgtagta aaaaactcaa agttccatta gggtacataa
     3481 gagaaaattt aaaatatgga tatatggatt actttggaga tgcaataaat tatttaagtg
     3541 aagtattaaa tatagatgaa tactgcaaaa gtgaatggag ctatctcgat aatagcaaat
     3601 ctccatctga gatatttaat attctaaaca ataaaatatt tagtataagg ctttctaatg
     3661 aaaaaagaaa tgaaattttg acaaatgata aaattgaagc attaaaaatg aattatagat
     3721 ttgaatgtat tgatgaagaa tatgatgaat attttaaaaa atataagtct ataatcaaaa
     3781 gaggtggaaa gaaaaaagtt gaattagtaa ataaaaaagg tgacatttta gaaatattta
     3841 agtctttaga agaatgtgcc atttatttgc aaaaggaaaa gaatgaagtt atacaaatgt
     3901 taaaatatgg agatacaaaa gtaggaagaa actttataag gtatagtttg agaagtattt
     3961 aatttcattt taaattcata agagtttcat ttctgattca cacatttaat attattttaa
     4021 tgtaaaataa taaaggtgaa gtattgcaca attaaaatta aggagggtat taatgtctga
     4081 agaaaaaaat ccaaaagaaa atacattaga tgaatctaaa aattcagata atgtaaattc
     4141 taaaaaagat gatgtgtcag atgaaaaaaa tactgataac aaagttaaaa aaagggaaat
     4201 aaagctaaac aacaaaacac ttaaagctat tgtagcaatt gttgcagtag gagctatatg
     4261 tttaggtgga ggctatgttt ttggcaaaga agctggaaga aaattgcctg ctacttcaaa
     4321 acactataat agcagtgagg tagtagctac tattggggat aaaaaaataa caggtgaaat
     4381 gttaagaaag agaatggagc cattatttta tttaaatgca aaaactacta tgactgatga
     4441 cgaaatacgt tcatatgaat caaatatgat tgaaactatg attacatctg atatattatc
     4501 ctctgaagca gcaaaagaga aaataagtat aactaatgac caactagatg ctcagtataa
     4561 taatttaatg agtagtatag aatcagcaat gggaatgact aaagaagagt ttttaaaaca
     4621 gtttgatttg actgaaaaat atataaaaga tgatatgaaa aaagagctag tagcatcaaa
     4681 atatttaaat gaaaaatcaa aagttactga taaagaagca gagaactatt acaataaaaa
     4741 caagagcaat tatttacaag ttagagcttc acatatttta ataaaaactg ttgatgataa
     4801 aggtaagcaa gttagtagta gcaaaaaagc tgaacttaag aaagaagctg aagaaatact
     4861 taaaaaagcc caagctgggg aagattttgc tactctagct aaaaaatact ctgaagatag
     4921 tagtgcagaa tcaggtggag atttaggttt ctttggtaaa ggtcagatgg tggaatcttt
     4981 tgaaaaagca gcctttgcat tgaagaaggg agaggtatct aacaaacttg tagaaagtga
     5041 ttatggatat catataatta agaaaactga tgaaaaatat cagccatttg aagaggtaaa
     5101 atcagattta gtaagcagtt taacatcaga aaaacaaaac ctacttatac aaaatctaaa
     5161 agaaaaatat aatgttaaaa ttacagataa atatcaaatg aaagctaaag aatagataaa
     5221 gtcaatttat taaaaaaaag aaaaattaac tgtatggata aaagctattt ttactctcaa
     5281 tatagagagt ataaaatagc ttttattatt ttgcaataaa atctatttat atatatgaag
     5341 tgataaagaa ataacatgca aaaaggtatt aaaaagaaat atttttaatt tgaatttttt
     5401 caataaaaat ttacaaaaat gaaaaaataa acaaaaaaga attgaagatt taagttgtta
     5461 tttaaatata tatatattaa gattttctgc tgaagatact aaaaaaataa tatttttcat
     5521 aattttaaaa ataataataa aaaaatgaaa atacaggcta taatctattg agtaaacata
     5581 gaaaagatga tattataagt atgtgctaaa atggttaaag caatattaat attaaattta
     5641 actataaaac aaaagggggc gtttttttat gtcaagtaca gaaagcgtat ctaaaaaaag
     5701 tggtcatccg aagagttttt ggcttatttg catgacaatt ttgtgggaaa gatttagtta
     5761 ttatggttta acagctttat tggtattatt ttttacagca agtgtagctc aaggtggttt
     5821 aggtctttct actcaagaag caacatctat gtttggctta tttacaggtc ttatatatct
     5881 tacaccatta gtcggtggct ggttagcaga taaagtttta ggtcaacaaa aatgtatttc
     5941 tataggtgca cttttaatag caataggaga tttcgtacta tttgcttcat ctacatctat
     6001 tgcaatggtt tgggtaggtc tatcaataat tattttagga aatggttttt ttaaatcaag
     6061 ttgctcaaat atagttgggg atatatatcc taaaactgat gtgtcaagaa aagatgcagc
     6121 atacagttta ttctatatgg cagtaaatgt aggagcattt atagctccaa taatatgtgg
     6181 attgctttct gataatgtat ttgcagtaag aggagcaagt ggagatatag tatcttatgg
     6241 atataagatg gcatttttaa tttgtggtat aggcatgatg ttaggtatga ttatatttac
     6301 acttcttgca ccaaaattat taaatgaagt cggaaaatat cctgtgacta aaggtaataa
     6361 aggtgaaaaa attgagtatg gaccacttac taagttggag aaaaatagga taatagcaat
     6421 ggttatttta tttttatttg tggtcttatt ctggacagca tactatcaaa cacaaagttc
     6481 ttttatgatt tatgtaaggg ataatgtaaa tagaactcta tttggatttg aaatgccagt
     6541 agcatggctt acatcactaa atgctatatt atgtgtggct ttatgcccag tattagctac
     6601 cctatgggtt aaattatctc ataccaaaag aggtgatttc tctattccag ttaaaatggg
     6661 attaggaatg ataataatgg gtgttgggtt tatagttatg gtatttgcta cattagctaa
     6721 tggtccagat ggaatggtaa aggctagtat attgtttata gtattgactt atatatttac
     6781 aacagtaggt gaattattct tatctcctat aggacttgca atgttcaata aattagctcc
     6841 agctaagtat gctacgttgt ctatgggagc ctggtatcta acaagttttt ttgcaagttt
     6901 attatcagga aagattgctg gttttacagc cacacttggt tttttacaga tatttggagg
     6961 aatagcaaca gttttaataa tatttggttt aatattattg ggaataagaa atgtattaac
     7021 aagattaatg gctatggatt tactaacaaa agaaaaataa ttcatgacga attattgtaa
     7081 ataaatgaat tactttaaat aaaggaagtt acatttaaat ataaaattaa atgtaatttc
     7141 ctctttgatt atgaaaaaaa gctttacaca aatagatatt ataattaaaa
//
LOCUS       DAYATN010000168         7183 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.171, whole genome shotgun sequence.
ACCESSION   DAYATN010000168 DAYATN010000000
VERSION     DAYATN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.171"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            517..1008
                     /gene="infC"
                     /locus_tag="ACGI8A_003354"
     CDS             517..1008
                     /gene="infC"
                     /locus_tag="ACGI8A_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFH0181318.1"
                     /translation="MNEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLDLVQISP
                     NANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTKANNAIK
                     FLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNNMVVIID
                     PKK"
     gene            1036..1230
                     /gene="rpmI"
                     /locus_tag="ACGI8A_003355"
     CDS             1036..1230
                     /gene="rpmI"
                     /locus_tag="ACGI8A_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFH0181319.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1286..1642
                     /gene="rplT"
                     /locus_tag="ACGI8A_003356"
     CDS             1286..1642
                     /gene="rplT"
                     /locus_tag="ACGI8A_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFH0181320.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1952..2497
                     /locus_tag="ACGI8A_003357"
     CDS             1952..2497
                     /locus_tag="ACGI8A_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181321.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            2932..4857
                     /locus_tag="ACGI8A_003358"
     CDS             2932..4857
                     /locus_tag="ACGI8A_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0181322.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5258..7162
                     /locus_tag="ACGI8A_003359"
     CDS             5258..7162
                     /locus_tag="ACGI8A_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0181323.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3023 a          714 c         1361 g         2085 t
ORIGIN      
        1 gaatataatg taggatttgt tatgtagtat aatagtatat tagagtacaa tatagaattt
       61 gttatgtagt atagtagtat attagaatac aatatagaat ttgttgggta gtatagtgtt
      121 atattagaat acaatatagg atttgttatg tagaatatat agagtcatgt agaatataca
      181 tatataaaag gattttgggt ataaatttaa aaattaatag caaaatgagt agtatagaca
      241 tataattagt agtaggaagt aattaaattt ttcctaaaca aaaaaaatag ttgcaaataa
      301 tttgattaag tgatatagta aataaggtaa gaataaaaca aagaagaagt ccgcttctca
      361 ccttacgccg gaggttagta aggtaaatat aagttaaagc ttaattttaa tttaagtttg
      421 tttaaatttg gtaacttata ttgcggatga ttacgtcatc cgctttttat tttataaaaa
      481 ttttggaggt gtcctacaat tagcaaagaa ttagcaatca atgaacaaat aaaagataag
      541 gaaataagag tcctttcacc aacaggtgaa caacttggag taatgcctac taaagaagct
      601 caagcaatgg ctaatagcaa aaacttagat ttggttcaaa tctcgccgaa tgcaaatcca
      661 ccagtatgta agataatgga ttatggaaag tttagatatg agcaagcgag aaaagagaaa
      721 gaagctaaga aaaaacaaaa aactatagtt gtaaaagaag taagacttag accaggtatt
      781 gaacaaaatg atttaaatac gaaagctaat aatgctataa agttccttaa aaaaggggat
      841 aaagtcaaag ttgaacttag atttagagga agagaattag gacataaaga cataggtaaa
      901 gaagttatgc ttaagttttt agatataata aaagagtttg gagaaccaac aaaagcccct
      961 gcatttgagg gtaataatat ggttgtaatt atagatccaa aaaaatagat gattaactga
     1021 gaggaggaat ttgtcatgcc taaaatgaaa actcatagag gtgcagcaaa gagattaaag
     1081 aaaacaggaa ctggaaagtt aaagagagct aaagctttta aaaaacatat attaactaaa
     1141 aaatctgcaa aaactaaaat gaacttaaga aaatcaactt tagtaagtga tggagatgct
     1201 aagagaatag cacaattatt accatactaa gatacagatt gtaaataata actatagaag
     1261 agggatataa aaggaggtca taacgatggc aagagttaaa aaagctatga acgctcgtaa
     1321 aaagcataag aaaatattaa aacttgcaaa aggattcaga ggatctagaa gcaaattata
     1381 tagaccagca aatacattcg taatgaaagc attaaaaaat gcttatatag gaagaaagtt
     1441 aaagaagaga gacttcagaa aactttggat acaaagaata aatgcagctg ctagaatgaa
     1501 tggaatatct tattcaagat taatgaatgg attaaaatta tctggtgttg aagttaacag
     1561 aaaaatgtta tctgaaatgg ctatacaaga cccagaagga tttgcaaaat tagcagaagt
     1621 tgctaaagca aaattagctt agtattgaat catgagatgt aaagtctttg agttatataa
     1681 ataattcaag gactttttta ttttttgaaa aatacttatt agtgtcatgt acaaccaatt
     1741 ttataatgat aaataaaata agcaatacaa tagaatttta tgagatttat tataagaatt
     1801 gttacaatta tttatgatag ctataaaaag tcatagtaaa aatgatattg atgtatgttg
     1861 atatatgtat aaagtttcca atcaatcttg aattaatatt atcaacatga taatattatc
     1921 ttattaataa tataaataag ggggacataa tatgaatgaa aaactaatgg attgttttgt
     1981 aaacccaatt aaatgcaagt tattgattga aattttttca aataatcgaa ctacagctaa
     2041 acaattagga gaaaaatttc aagatatatc aaaggcaaca ctttatcgtt accttaataa
     2101 aatgttgaaa gatgatatta taaaagtggt tgaagaaaat aaaataaggg gaactgttga
     2161 aaaagtatat gctatgaagt tagatatagc tgatgatgtg aacaaaatta tagatgagaa
     2221 ctctggagaa gaatatgctc aactgtttac tcaatatatg atgggatttt taagagaatt
     2281 ttatgaatac gctgaaagaa aggaaaagga tatcctaaaa gatggttcag gattttctgt
     2341 ctgtccagta tatgctagta ttgatgagat agttgaagtg agtaaaaaaa ttgctagtat
     2401 actagaacct ctaagaaata actcacatga agaaggtaga agaatgcatt ctataggagt
     2461 gataattact ccacctaaaa aggatgaagg gaaataaatc tgatttacat ggggtagagc
     2521 caatttatac agggaatcgt tttatgattt acatatgtat ccagagcaaa gtatctctgc
     2581 ttggattgta cttcattgta ccatttcatc caatgacttg gagtggtaca atgaagtttt
     2641 ttaatttttc atattaatgc taaagaaatg aactttagac cacatatgca attaggttgc
     2701 attatacaat tagaccacat atacaaaata aaaatcagta tttttatagc agataaattg
     2761 actgtcattc ttcttaaaaa tcatctatta gttattgtta gcatattgaa agaaattata
     2821 gtaatctatt gaataaatga atattgccta taatcatata aactgctata gtttaaaata
     2881 aagcaggacg ttaaatttta tagtaatcta aaaatttaag agggggattt tatgaaaata
     2941 aaatttgtta ttatgccatt ggtgatattt ttaatggtat tatttgtggg gtgtcaaagt
     3001 acaaaagata cagaagttaa agcaaaagat acagaaatta aaatattagg gacaggtgat
     3061 ttacattcaa ttttgacaga ctctatggtt tcgtatgtaa atgaggaaag ggaaaagaat
     3121 aaaaatctgc ttatggtaga tgcgggagat ttctatggta cacaaagtag agaaatgtgg
     3181 gaatggtctt ctggaaaaaa gttaattaat attaaaagaa atggtagagc agagtatgat
     3241 gacataatta agagtagtaa agatgaagta cctatagtta aagatatggc aaagttgaaa
     3301 tatgatgctg tagttttggg agataatgaa tttgtttcaa atgataagca aagtcttgat
     3361 aaactggttt ctgattttaa aaataataat atgccattgg tatcagcaaa tatatatgaa
     3421 caatctggag aaaattatgt acaaccatat gtaatgaaaa atattaagac agatgagggc
     3481 aatgtaaaag ttggaatact aggtcttact ataaaagaag taggggaaac ccttggtctt
     3541 gaggattttg aaaaggataa aaaagctaga gagcttggag aacaagctga ttacaaagga
     3601 aaattatatg caaatgattt agtagaagat gcaaaaaaat ggataaaagt tatggaaaaa
     3661 gaaagtccag acataatagt tgcagttgta cactcagggg aagaacctaa aacagataga
     3721 aatccaggaa atagaataaa agagttagca actacagtgg atggaataga tgcaattgta
     3781 gctggtcata ctcatgaaaa aatagatgaa cacaaatata aaaataaatc tggagatgaa
     3841 gtaattgtta cacaaccagg ttggcatgga gatagagtat cagagattaa tttcaaacta
     3901 aataaagaga atggaaagtg ggacataaag gaaaagtcta gtaaattaaa aataattgat
     3961 aaagaagtaa gtatagttgc tactgcagat ttacattcac atttctctga taaattaaca
     4021 gttgaccttg ttaatgaacg aagtaaccca attgaacctg ttgtagtaga tgcaggcgac
     4081 ttcttagacc ctcaaacaga tgaaatgact caatggtata aagagtggaa aagtataaga
     4141 gataataata gtgataaaac gattagtaga tgcccaatgg tcatggatat gaatcaggga
     4201 atgtatgatg cagtagtact tggaaatcat gaatttgtat ctgaaaaaga tgaattttgg
     4261 tcagatgaac gccttttaga tgctgttata gaggattttg aacaggtggc aatttctgtg
     4321 ttatcggcaa atatatatag gcaatctggt aaaaattatg ttaaaccata tattataaaa
     4381 gatgtggaga cgagtgaagg taatgtaaaa gtaggaatat tgggacttac tataaaagaa
     4441 gtaggtaaag gtcttaaaaa tgtaaactta caagagcaat ttcaatataa ggataaattg
     4501 tatgcaaatg atttagtgga agatgcaaag aaatgggtaa aagttatgaa aaagaaaaaa
     4561 ccagatataa tagttgcagt tgtacattca ggagaagaac caaaaaatcc aaaacatcca
     4621 ggaaatagaa taaaagaact agcaactaca gtagatggta ttgatgcaat tgttgctagt
     4681 catactcatg aaaaaataga tgagcatgaa tataaaaata agtctggaga aaaagttata
     4741 gtgacacaac caggtgagca tggtaaatat tattctaaaa ttacctttaa agtaaataaa
     4801 gaaaagggtt cgtggactat taatgataaa ttcagtaaaa caattgaggt tgaataactt
     4861 tataaaattt atagataaag taaattatct gatattggca tgatatataa gataaagttt
     4921 ctaatattag tataatgcat aatgtgaaat attttaataa taagagaata aacaagacaa
     4981 ataaaaatat tttaacatgt aagatatagt atgaactatt atactaatgc ttactttata
     5041 atttgaacat gttagaataa gtcttatttg aaaattttag tgtactagag taagtattat
     5101 tttactagta aaaaatattg agaactatat gtagatttgc ttaaaaagga atttacatat
     5161 agttcttttc gtataataaa tagtacaatc ttatatggta aagataaagt gtgatattaa
     5221 attttattat catccaaaat ctaaggagga gtctcttatg aaaataaaat ttattatagt
     5281 agcatcaata atatttttaa tagtattact tgtggggtgt caaagtacta aagacacaga
     5341 aatcaatgta ctcgccacaa gtgatttaca ttcaattatt ccagataatt taatttccta
     5401 tgtagaagag gagagaaaat atgatgagaa tctacttatg gtagatgcag gagatttttt
     5461 tgacatgcaa agtggtgaga tgaataagtg gtttactggt caaaaattag taagatttaa
     5521 agatgggata ccagagtaca aaaaaatagc tgaaccacgt gaaggagaag ttccattggc
     5581 taaaaaaatg gcaaagctga agtatgatgc agttacattt gggaatcatg aatttgtatc
     5641 taatgacaag cagagccttg ataaattggt ttctgatttt aaaaataaca atataccatt
     5701 agtatcagca aacgtatatg aacaatctgg agaaaactac gtaaagccat atgtaatgaa
     5761 aaatataaat actgacaagg gaactgtaaa ggttggtata cttggtctga ctataaaaga
     5821 agtaggggaa cgttcaaggt ttaaaagttt tgaacaagat aaaaaggcta gagaacttgg
     5881 ggaacaacct caatacaaag ggaaattata tgcaaatgat ttagtagaag atgcaaataa
     5941 gtgggtaaat gttatggaaa aaaaagaaaa gccagatata atagttgcca ttgtacattc
     6001 aggtgaagag ccaaaaaatc ctaaaaatcc aggaaataga ataaaagaac tagcaacaac
     6061 tgtagaggga atagatgcta ttgtagcagg tcatacacat gaaaaaatag aacagcatac
     6121 atataaaaat aattctggtg aagaagtaat agtaacacag cctggagctc atggtaatag
     6181 tatatcaaag attaatttta agttggataa gaagaatgat aagtggttta taaaagacaa
     6241 atatagtgaa ttaaaggttt ttggaaagga agtaaatata attacaactt ctggtctgaa
     6301 tccaaaattt tcagatgaaa tggcagttaa tttgtttaat gaaagagata gagatgttca
     6361 acctatcttt atagatactg gagatttttt aaatactaat acaaaagaaa tgactaagtg
     6421 ggctaaagag tggcaataca atgaaaataa aggcatctat aaaaaaataa atgaatggcc
     6481 aattgtatat cttggttatg atgctgtagt acttggtagc catgaattta cagaggataa
     6541 agagactttt gaggaaaaaa aatctacatt ggattatatt atagaaaact ttgaagaaat
     6601 gaagatacca gtattatcag ctaatatata tgatgaatct ggagaaaatt atgtaaaacc
     6661 ttatataatt cataatgtag aaactaggga aggaaatata aaggttggag tattaggtct
     6721 tactactact gataaggatg aagaagactt aagtaactca agcttaagca aagactcaaa
     6781 gacttcgaaa attcctgaag aaacaggaaa gttatatact aataatctag taaaagatgc
     6841 aagcgaatgg gtaaaagtta tgcaaaagga aagtcctgat gtgattgttg cagttgtaca
     6901 ttcagataat aatagtactc taaaaaatcc aagtagtgaa gtcaaaaaac ttgcaatgtc
     6961 agtagatgga atagatgcta ttgttgtagg tcatacgaaa aataaggtag aagaacatat
     7021 ttacaaaaat aagtctgggg atgaggttat agtaacacaa tctggagaag attgttattc
     7081 aaaaataagt cttgaactaa gaaaagaaaa tggcaagtgg aatatcataa acaaatatag
     7141 taaagcaata aaaatgaagt gaatttattg atatataaac cat
//
LOCUS       DAYATN010000169         7051 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.172, whole genome shotgun sequence.
ACCESSION   DAYATN010000169 DAYATN010000000
VERSION     DAYATN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.172"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(350..1240)
                     /locus_tag="ACGI8A_003360"
     CDS             complement(350..1240)
                     /locus_tag="ACGI8A_003360"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFH0181324.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(1270..2535)
                     /locus_tag="ACGI8A_003361"
     CDS             complement(1270..2535)
                     /locus_tag="ACGI8A_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HFH0181325.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(2645..2747)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2801..5488)
                     /locus_tag="ACGI8A_003362"
     CDS             complement(2801..5488)
                     /locus_tag="ACGI8A_003362"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFH0181326.1"
                     /translation="MKKRKEWSSKMENHKQMPWHSRATHEILESLDSSEEGLTDTEAE
                     KRLKENGLNELRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIV
                     TVNAVIGIVQEKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAM
                     VPADLRLIETSSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGR
                     GVGVVVATGMDTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGA
                     FYQRPLLPMFMIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGG
                     ATVICCDKTGTLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCN
                     DACINPDKTDEILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVH
                     NIDEKIIAYTKGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGF
                     AKREISSIPKEDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHK
                     VTATTIAHELGIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSL
                     KRIGEVPAMTGDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIK
                     EGRRVYRNIQKVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALA
                     LGVDPPSRNIMKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQ
                     TMAFCVLAFSQMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRN
                     AFNLTVLNNLEWLVTIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(5586..6329)
                     /gene="yaaA"
                     /locus_tag="ACGI8A_003363"
     CDS             complement(5586..6329)
                     /gene="yaaA"
                     /locus_tag="ACGI8A_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFH0181327.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            complement(6339..6803)
                     /gene="bcp"
                     /locus_tag="ACGI8A_003364"
     CDS             complement(6339..6803)
                     /gene="bcp"
                     /locus_tag="ACGI8A_003364"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFH0181328.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
BASE COUNT         2204 a         1260 c          823 g         2764 t
ORIGIN      
        1 attgaaattt tgttatctta ttgatattta attttataca ggatattttt aataaaattt
       61 aataaaaaat ctatatatat attttacact gatttctccc tttttaatct cttttaaaag
      121 ttaaaataca aatttattga tactataaag gtttagtctc ccattatcgc accatatata
      181 aaattaaagc cacctctatc tacatacacc taaagtgatt ttcttaaaat ataaaactac
      241 tattataagg tcaaatttta aaaaaataag gtataaagta tcattaagat taactttata
      301 cctataaatt tctatatttt tttagttaac atttatatac tcttaaaatt tattgtatca
      361 aatagttttt tatccaattt ctaaacaaca attctgaatg ttttttccat tttacagttg
      421 gctcttctga agggtcatca ttaatatagt aattttgagg tatagatatt ttatctcctt
      481 tatttatatc cctcttatat tctttatcaa gagtgtattt gtcatactcg ctatgaccca
      541 ttacgaacac atctcttgaa tttgtaatta tatagggacc agcttcttta gaatgactca
      601 caatgctcaa ctctttattc tctttcaaag cttcaatatt tacagtagta tgtcttgaat
      661 gtggagcaaa aaattcattt tcaaatccat ctactatttt actatcttta tctaccttat
      721 gcttaaatac tccaaaacat ttttgactta gtggaagttt ttttacattg tagtatttgt
      781 aaagacttgc ttgagctgcc caacatatat ataatgtaga tttagttttt ctcttagaat
      841 aatccataat ttcttctagt tcattccaat aatcaacttc ttcaaactcc atttgttcaa
      901 ctggagctcc tgttattata aagccatcaa aatactctcc tttggcttca tcaaatgttt
      961 tataaaattc tttaacatat tcattacaac ttcttttact ttctcttgtt gaaattttaa
     1021 taaattccac agacacgttg aatcctgttt tatctatcct tcttagtaaa tctaattctg
     1081 tatctatctt tataggcatt agatttagta tagctatctt agtgtctatg ttttcttcat
     1141 actttaattc ctttttaaaa tcttctttat aaattacatc tattccttca tctaataatt
     1201 tattaataac aggtaatccc tttggtagta ttaaagccat atcttttcac ttctctttct
     1261 cttagaatat tagaaattta aagcttgatt taaatcagct attaaatcct ctacattttc
     1321 tattccaaca gataatctta ttagtgaagg caatactcca gatgcaatta attgttcttc
     1381 tgttaattgt ctatgagtag ttgaagctgg atgtataaca caagttctta catctgaaac
     1441 atgtgtaacc aaagatgcta tctgtaattt ttccacaaat tttttggcat tttctaaccc
     1501 acctcttact ccaaatgata aaacaccact acatcctctt gataaatact ttttggcatt
     1561 ctcataatac ttgttatctt caagtcctgg gtaattaatc caatctacat tttcatgttt
     1621 ttctaaaaat ctagctattt taagtgcatt ttcactatgt ctctccattc taagatgtag
     1681 tgtttcaaca tttaaattag taagatacgc attgaatggg cttaaacaat ttccatagtc
     1741 tctaagcaac tgaactcttg ctttagttgc atatgcggca tttccaaatt tttgagtata
     1801 gcttatacca tgatatgttg ggtctggctc aactaattct ggaaattttc cattatccca
     1861 gttaaagttt ccaccatcca ctataattcc tccaacactt gaagcatgcc catctaaata
     1921 ttttgtggta gaatgagtaa ctatatttgc tccatacttt aaagggttac aaagcactgg
     1981 agatgctaat gaattatcaa caataaatgg aacattaatt ctcttagcta catttgctat
     2041 tttttcaaaa tctatgactt caagagttgg atttgcaagt gtttctgcaa acacaacctt
     2101 agtattttcc tttgcaagtt ctactatctc atcttcactt gaatctaagt caaacgatat
     2161 taaatctata ccaaattttt taagactagg tcctagtaaa ttgaatgttc ctccatatac
     2221 ttttgaagaa caaagtatac tatctcctga tttacatata tttaaaactg ccaacatatt
     2281 tgcagattgc cctgatgata cagctacaga agatactcca ccctctagta aacttatttt
     2341 ttcttcaaaa gcttgaatag taggattgct tattcttgaa tatatatgtc catcaacttt
     2401 taaatcaaat aattcagcaa gttggtctat actgctatat ttaaaagttg tactttgata
     2461 taaaggtagt actcttggtt ctccattacc tggtttataa tttccttgca cacatattgt
     2521 ttctttatta tacattcttg tatccccctt agattttagt aaaaaaaatt aaactattta
     2581 ccagatgaga gttaaaataa ataaagcctg agtatataac tcaggctctg tataagctta
     2641 attatactca tctctcagct ttcgccgcag gatttagcac caatgtatat aaatatactg
     2701 gttgccgggt ttcatagggc cagtccctcc acctctctgg ataagattta tttaattttt
     2761 ctatgtttta tatcttatat atttttccta attttgtcaa ctattttttt aatttttttc
     2821 ttttaataat tttcccaacc tctacttgaa ttacagacat tatggctaaa acaatcgtta
     2881 caagccactc taagttattt aaaactgtta agttaaaagc atttctaaaa acaggtacaa
     2941 atagtattac tgctattaaa aatgcagaag ttaaaaatga gattaaaaga aatggatttt
     3001 taccattatt tctattaaac attgaatctg tatttgaacg ctgactaaat gctcgtaaca
     3061 tctgtgaaaa tgctaataca caaaatgcca ttgtttgacc tacagaatga ttttctatat
     3121 taagaccaat tttatatgct aaaatagtca ttattgccac aaatatacct tgggtaacaa
     3181 ctctttgtat aagatttttt tcaaacaaag tggttgattt cactggctgg tgcttcatta
     3241 tatttctgct tggtgggtca actccaagag caagggctgg aagcgtagca gttgcaaggt
     3301 ttacccatag aatatgaaca gcaagaagcg gagtatccca atttaataat attgctacaa
     3361 acaacgtaag tatctctgct atattaccaa caagcaaaaa ttgtataact ttttgtatgt
     3421 ttctatatac acgtctacct tcttttattg cataagcaat tgttgtaaaa ctatcgtcca
     3481 ttaaaatcat gtcagaagaa tcttttgcaa catctgtacc acttataccc atggctatac
     3541 caatatcagc agactttaag gcaggagagt catttacacc atccccagtc attgctggta
     3601 cttctccaat tctttttaga gattgtataa ttctaagttt atcaaatggg gatacacgag
     3661 caaatactgt agtattttta acagcttcat ctagttcatc atcactcaaa ttatcaagtt
     3721 catcaccaga tatcacagta ttttcttcac tccatattcc taactcatga gcgattgttg
     3781 tggcagtaac tttatggtca cctgttatca ttatggttct tatacctgct tctttacaag
     3841 tacttactgc atcaattacc tcagttcttg gtgggtcaat cataccaacc atacctataa
     3901 atgttaaatc atattctata ttttcactat cttcttttgg tatagatgaa atctcacgct
     3961 tagcaaaacc aagtacacgc aatgcatctt tagacatgtt aagacataat tcgtgtatat
     4021 tctttttatc cacttcagta atgtttcttt caccttttga agttaatatt ttagtacata
     4081 ggtcaagaat ttcgtctact gctcctttag tataagcaat aatcttttca tctatattat
     4141 gaacagttgt catacatttt ctatctgaat caaaaggctg ttcaaataca cgtggatatt
     4201 cttcttcaag agcttcttga tctattccaa attttttggc cataaaaatt aatgcgccct
     4261 ctgttgggtc acctaatatt tcatctgttt tatctggatt aatacatgca tcattacaaa
     4321 gtgcaccagc ataaacaatc tctttataca ctaatggatg tttttcagat gcagtatcta
     4381 ttgatgtcac ttttccagat tcaaaatctc cattgacagc aatatgtgta acagtcattt
     4441 tatttaaggt taatgtacca gttttatcac aacatattac tgtagctcct cctaatgttt
     4501 caacagcagg taattttcta attaaagcat ttcttttagc catacgctgt acgcctaaag
     4561 ccattacaat agtagctgtt gcaggtaatc cctctggaat aattgatatg gcaagggaaa
     4621 ttgcaatcat aaacattggc aagagaggtc tctgataaaa tgcacctatt gcaaatataa
     4681 caacgcatac aataatacct atgacactta atgtctttcc aacagtatta agtttacgtt
     4741 ttataggagt atcaaaatca tcttgtgaat ctaaaagatg tgctatgtta cccacttctg
     4801 tatccatacc agttgctact acaacaccta cacctctacc atatgtgact attgaagatg
     4861 tatacgccat attgctacgg tctcctaata cacattcttt aggtaagatt gtatctgcat
     4921 ctttttctga tggaacagat tctcctgtta aagaagcctc ttgaattttt aaacttgatg
     4981 tttctattaa acgaaggtct gctggaacca tagctccatc ttctaaaaaa acaatatctc
     5041 ctactacaag attacttgct ggaataacat tttcttcacc atcacgcata acacgagctg
     5101 taggtgaatt catatttttt aatgcttcta atgatgattc tgcttttttc tcttgaacaa
     5161 ttccaataac tgcatttact gttacaatta ttagtattac aaaagcttct gtaaattcgc
     5221 caaatatcaa agataatatt gctgctccta ttagtatcat aaccatagca tctgtaattt
     5281 gactccaaag catttgtaaa gttgttctag gtggtttttt tctaagttca tttaagccat
     5341 tctctttaag ccgtttttcc gcctcggtat cggtgagccc ctcctctgat gaatctagac
     5401 tttctaaaat ttcatgcgtt gctcttgaat gccaaggcat ctgtttgtga ttttccattt
     5461 tggaagacca ctcctttctt tttttaattt aagaataatt atattttaaa atcaagttca
     5521 attcaataca cactaagtat attttaccta aatttaacaa tgccatttaa tatttaacaa
     5581 aacttctact cttttacaaa tatgtattca ctattgttag ataagttttc tttatactta
     5641 taaccttcta aatcaaactt tttcagttct tcaatatctc ttatcctatt ttttacaatg
     5701 tattttgtca tcatacctct agctttcttt gcttgtgtag attccacttt caatatgcca
     5761 tttttattta ctttaaaagt acatgtaata tatgtatcac tatctttaat aaatttttct
     5821 atggatttgc tgtattcatt tgatgaaaga ttaacgatag ctgttttttc tttgattagt
     5881 tctttatata tacgatttcc ccaatattcg tataaattct taaactcacc tacttgtaat
     5941 tttgtctgca tttctaatct atattcatat atactatcat atggttttac aacaccatac
     6001 aatccactta tgattcgaat acagctattt gcaaactctt catctttcct tgtaaaattt
     6061 tctgcttcta tatttttata ttgtattcca tcataagtta agattgcagg gtttcctaat
     6121 ctatcaaatt taatgctttc aaatctattt ttatttaata cagctaattt ttcattaatt
     6181 ttcattttat ttttaaaatc ttctatacca aatgtcttta tattttccac tatttctttt
     6241 gtgttatcta taaagcaagg caaactcaaa tctctatcaa atacttctat attcttcata
     6301 tttttagctg gtgataacat tataatcata aattcttatt actcctgttt ttccaaatat
     6361 tcaagaatct cttgtgcatt tgtgtctggt tttgcttttt caaagacttt ttcaataatt
     6421 ccattttcat ctataatata tgtcgcacgt acaactccca atgcagtttt tccatacatt
     6481 tttttctctt tccatacatc aaaagctttt atagcaacta agtcagggtc agaaagtaaa
     6541 ataaaaggaa gttcatgctt ttctgcaaat ttttgatgtg attttatact atctttactt
     6601 atcccaataa cttgtatatc ttccttttta aatccatcat atgcatttct aaaagcacaa
     6661 gcttgacgtg tacatcctgg tgtattatct ttaggataaa aatatactac tacttttttg
     6721 cctttaaaat ctgacatact taccttattt ccatctttat cttctagtgt aaattctggt
     6781 gcttttgttc ctatactcaa cataaatatc tctccttttg tttttattaa taattttata
     6841 ctatgcttat attattaccc tataaatcta tatataaata aaatatttta ttttttatgt
     6901 ataaagtttc ataaatatat aattttcatg tatttatatg ttaaaataga ctgaaaatag
     6961 ttttattaag atatctatgc ttaaaacaat gttaaatgat agattaatta aaataaggat
     7021 atctaaaaat aaaattttta gttcatgaga t
//
LOCUS       DAYATN010000170         7039 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.173, whole genome shotgun sequence.
ACCESSION   DAYATN010000170 DAYATN010000000
VERSION     DAYATN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.173"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..854
                     /locus_tag="ACGI8A_003365"
     CDS             <1..854
                     /locus_tag="ACGI8A_003365"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HFH0181329.1"
                     /translation="LKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(987..2600)
                     /gene="ggt"
                     /locus_tag="ACGI8A_003366"
     CDS             complement(987..2600)
                     /gene="ggt"
                     /locus_tag="ACGI8A_003366"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFH0181330.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2825..4762)
                     /locus_tag="ACGI8A_003367"
     CDS             complement(2825..4762)
                     /locus_tag="ACGI8A_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181331.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEVEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVAKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4749..5516)
                     /locus_tag="ACGI8A_003368"
     CDS             complement(4749..5516)
                     /locus_tag="ACGI8A_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181332.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5804..6478
                     /locus_tag="ACGI8A_003369"
     CDS             5804..6478
                     /locus_tag="ACGI8A_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0181333.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6502..>7039
                     /locus_tag="ACGI8A_003370"
     CDS             6502..>7039
                     /locus_tag="ACGI8A_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0181334.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDF"
BASE COUNT         2466 a         1034 c          835 g         2704 t
ORIGIN      
        1 ctttgaaaaa cattatttca gcaaaagaac ttatatcaaa actagaaaaa aatgataatc
       61 tagttgtaat agattgtaga tttgatctta tcaatagaac ttatggtatt gattcttata
      121 aaaaaggaca catcaaaggc agttttatct tagatataga caaggattta tcatctccta
      181 caaaagaaca tggtggtaaa aatcctttac aagacccttt aatactaaaa gaaaaattag
      241 aaaaaatggg tgttgataat gacactacga ttgtaactta tgatgatgga gatttaaatg
      301 gagcttgcag attatttttc caattaaaac atttggggct taaaaatgtc tatgtactag
      361 atgggggaat aacatctttc gtaaaggaag gtggagaatt ggaagagaaa gtgaatatac
      421 caagttgtac aggtaaggaa attagaccaa atataaacaa tgatttagtt gttccaatgg
      481 aatatgttaa atctaaatta tacaaaaaag atactgtaat aatagattgc cgtgctaatg
      541 agagatacca gggtttagtt gaacctgctt actcaaaagc tggtcatata ccaagtgcaa
      601 aaaattattt ttgtaaggat ttaataaaaa gtgactttga aaatggctca cttaaagata
      661 tagaattttt aaaaaaattc tttaaagatt taaataatta tgatgaagta attttatctt
      721 gtggttcagg gatatctgct tgtgttaaca gtcttgctct tagagaactg gatatacctc
      781 acaagattta tattggtagc tttagtgact ggattagtta tgatgacaat gaaatcaaaa
      841 ctggtcaaga ataaggctca taatctatat aaaatcagca tatgatagct taaattttgt
      901 aaaggcgtca gtcattgtag actttcgcct ttacttatta tttcatatac tctttaaatt
      961 tattatatat attttagttg ataatattaa taaactgcaa tatgaccatc acatcttgat
     1021 tccgtcccac aaatataact ttcctgttct gttttaaata ttatttgacc tcgtcccatg
     1081 tcgacagtat catgaattac ttttatttca tgaccctttt caattaatga atctattata
     1141 tgctttggca tctctggttc aacttctatt tcttttcctt ttatccactg ccatcttgga
     1201 gcatctagcg cctcttgtgg atttaatcca aaatcaatca tatttgttaa cacttgaata
     1261 tgtccttgtg gttgcataaa agctcccatt acaccaaatg gacctattgc tttatcttct
     1321 ttacctaaaa atccaggtat tatagtatga taaggttttt taaatggttt tactacattg
     1381 tggtgtttag ggtctaaatt aaaattattt cctctactat gtaaagcaat cccagttctt
     1441 ggtatcacaa ttcctgaacc aaaatttatg taattgctct gtatatatga aaccatattt
     1501 ccatccttat ctgcagtaca aaggtatact gtaccaccac aatatggctc tcctgcagtt
     1561 ggatacaaag cctctttatt gtcaatcaat aaacttcttt ttttagcata ctcttggctt
     1621 agtaattcct gaatctttac cttcatatgc tctatatcag ttacatatgt tttagaatct
     1681 gcaaaagcta attttaaact ctctataatt ttatgtatgt cttctacatt ttctatattt
     1741 ccttctaatt ccaattcttt taatatgtta agtgccatta aaactgttat tccatgacca
     1801 tttggtggga tttcaaatat tttatatccc ttatattctg tacttatagg ttctacccat
     1861 gttggataaa aacactctaa gtcactcttt cttatagccc cattaaattt tttagaatat
     1921 tcatctattt tatcagcaag tcttcctcta taaaagcttt cagcctgagt atttgctatc
     1981 tcttctaatg tacttgcctg ttcttcacat ataaatatat ctccagcctc tggagcttta
     2041 ccatctttag aaaatgtatc aaaccatggt ttgaactctt ctccaataag ttctttttca
     2101 tagagttcat atgatttttt ccatacctta gctacattag gagatactac gtatccctct
     2161 cttgcatagt ttatcgctgg agataagcat tccattaagc taagtttacc atatttttta
     2221 tttaattctg accaagctgc tggtattcca ggtacagtta ctgctccaaa cccatatttt
     2281 ggcatttctt tcatattatt ataatttttt aattccatat tttctggagc aaagccacta
     2341 gaatttaaac catacaatcg tttttcttcc tctatccaaa caagggcata tgcgtctcct
     2401 ccaatcccat tgcttgtagg ttctactact gctaatgtgg cggctgttgc aactgctgca
     2461 tcaacagcat ttccaccctt ttttagtatt tcaagtccag cttgagatgc taatggtgtt
     2521 gatgttgcaa ctgctccatt tttagcatac acgacatttc ttattgatga gtatttatgt
     2581 tggtaggcat taaacttcat aaatttcagc tcctcgtcat tttttatttg atattctgct
     2641 tagatttatt atataacatc aagtaatgta actgatacaa aatgatataa aatatagact
     2701 ttttcctaac tttaattttg tctttaaata aatatcttaa actcaattct agtattttct
     2761 atctgcactt aaataagtta ttttattcaa aacatatatc taattctttt gacttaaaaa
     2821 taatttattc ttttatcatg tttagagcat attttaaagt taaaaaataa tatataaaat
     2881 atcctataaa ataaatgact agagttatga taattggtac aagtaatgac cttggtataa
     2941 acttttgtgc tatagacata gcaaataaat tatgaactgt acctacaact aatggcatta
     3001 aaaatataac ttttaactgt tttgatatta ttttctcaat atctttatta tttgctccaa
     3061 gttttattaa cataatatat ctttccttat catcatatat atttgataat tgtttaaata
     3121 agattatact acctgtacat aaaagaaata ctacagacaa aaatatgcct ataaacagca
     3181 ttagaccact taattttatc aagttattat aactttcaat acttgatgta aaattaaaag
     3241 gataagtaaa tttcatattt tcacttacta tattttttaa ttttaaagtc aaatccagtt
     3301 cttttttttc attttttata tcaattaatc ttattaatct agaatttcca tgtaatttta
     3361 actgattaaa tacgtcatct tttgctacaa ttaaatcaaa cgtagcttgt gaatttattt
     3421 ctggctccaa gatgcttttt tgtactttaa acagatttat atcatactca tttgtatctt
     3481 taatatgcaa gtttattttt tcaacctcaa agtctttaaa aaatattttt ttgtatgaat
     3541 cagaaacata gtacacagat tcctttgaag ttaattcttc ataattgtta ttctgatatt
     3601 gtcttaattt tttaaaatca ctttctctaa taatatctac agacgttcta tcttctatat
     3661 aactatttcc atttttataa tacatttcaa tatttatatc tttgtctata agttctattg
     3721 ttttatcaaa gattatctta ttatcttttt tatatttata taataatttg tcaatttcgt
     3781 tatttacata agaatttcct aaatttatgg tataactgta tttataattt tcacttatgt
     3841 tcctatctat atcgtaataa agtgatatgg taaaccctag catagttaca cttgtagcta
     3901 ttaatattgc tattacagcc aatacactac tattgctctt taatttaaat ccaagttcag
     3961 aaaatgcaat taaattctgt cccttataat aaaaactttt tctcttctta acagcatcaa
     4021 taatcatata catcgttgat gaaaaaaata aaaaagttcc aggtattatt gtaactaata
     4081 taaaaactga aagcataata ttttcagcaa taaaataact aattgacatt aaataagaaa
     4141 tagctattaa taaaacacca agtacacatt ttaactttat aaatttttta aacttatact
     4201 tttctggata tttattaaat aactctataa ttctcttatt tctgataact atagtatttc
     4261 ttattccaat aactatgaat attattaaaa ataccgctag tgtctgaact aaggccttta
     4321 tacttaagtt catatttact actatcattt cattcattaa ttttacaagc atcatagtca
     4381 taagctttga aaataacagt cctaaaaata tacctattag tattgcaact atacccatca
     4441 aaaaagtttc atataaaaat atttttccta tctgattttt cctcattcca agtaaactat
     4501 aagttcctac ttctcgccta cgtctattga aaaaaaatga gtttgaataa tatataaata
     4561 aaaatacaaa taccactatt acaattgaac ttgtattaat actcatccta gttttctctc
     4621 caagtgaact caacgcttca ttatactcta tagatttaaa actaaaaaat acaaatacac
     4681 taaatacaat tgaaataaag tacattaaat aattaaaaaa actattcttt acattttgca
     4741 ttgcaattct aaataaagtc atttttatta actcctccaa ttaaggaaac tgtatttact
     4801 attttattat agaactctct gttactttct ccctttacaa tttctgtgaa tattattcca
     4861 tcctttataa agagtattct attgcaaaaa ctcgcactta tagcatcatg agttaccatc
     4921 aatattgttt tattgctgtc tttgtttaat tttaatagac aattcaacaa atccctagca
     4981 gatttagaat ctaatgctcc tgtaggctca tctgcaagta ctaatgatgg ttttgttgct
     5041 atagctctac atacagctcc tctttgcttt tgtccaccag aaacttcgcc agggtattta
     5101 tctaatatag attctatatt taattctttt gaaatctctt ttattctatt ttctattact
     5161 gatactcttt cattttttaa agaaagaggc aacattatat tttcttttat tgtaagtgta
     5221 tccaaaagat tagaatcttg aaatacaaag cctagattat cacgtctaaa atctgccaat
     5281 tcattttgct tcattttaat agtattcttt ccatttatat aaatatctcc tgatgaaggt
     5341 aaatctattg tagaaataat atttaaaagt gttgatttac cagcaccgga agaccccata
     5401 ataccaacaa attctccttt ataaaccttg aaagttattc ctcttagagc ttcatgtttt
     5461 gaaccttttc ttccatactc cttttttata ttttcaacag ataatatttc ttccatagtt
     5521 aataatcacc tttcatcaca attagttatt tatcaattct cacaaatata ttatatatat
     5581 taaaatggct tttaaacatt taattatatt aatttaaggt tacattttcg taagtattaa
     5641 ttatatttct tcaaatttat tgcaattatg tttaaaatat aatttatcaa gtaaaatata
     5701 gttatattaa tataattatt ttaggtaata aggtagaaat attttaattt atcaagtaga
     5761 atatagttat gttaatataa ctattttgaa ttaggaggta agaatgttta aaataatggt
     5821 agttgaagat gacgtctcat tgaaaaatat catagcaaag tgtcttacta aatggggaca
     5881 tgatgttcat caaatagaaa atcttgaaaa tataatagaa gagttcaaaa actataaccc
     5941 agaattagtt ttattggaca taaaccttcc attttatgat ggttttcatt ggtgtaatga
     6001 aataagaaaa atttcaaaag ttcctataat ttttatttct tcaagaaatt ctaatatgga
     6061 tgtaataatg ggtgtaaatt taggtgcaga tgattatata caaaaacctt tttctgtaga
     6121 tgtcttagta gctaaagtta atgccctttt aagaagaact tataattttg ttgataataa
     6181 ctcaaatcag attattcaca atggtgttac tttagattta tcaactgcaa caataaacta
     6241 tgaagataat actattgagc taacaaaaaa tgaaatcaaa attcttcatg agcttatgaa
     6301 atataagggg cagatagtaa gtaggaataa actcatgaaa aaattatggg ataatgattg
     6361 gtttgtagat gataatacat taactgtcaa tgtaaataga atacgctcaa agttaaatga
     6421 aattggttta gaagatttta tagaaacaaa aagaggattg ggttatataa tcagttaaaa
     6481 caattctaag taggtgaatt tatgagcttt aaagattttc tcaaggataa actaggtttt
     6541 ttagtataca attttacttt cttaattttt acagtatcag ttattatatt ttctcctgta
     6601 gatgtttttc tgactgatac tattttatat atattagttg taaatgttgt gtttttattt
     6661 ttatatctat ttattagcta catcaagaag aacaaatttt tagataagat aaaaaatgat
     6721 acattcaaaa ttaatatgaa tgatatagaa ttagctagat gtaaaaatga agaaaaaata
     6781 tacttgaaca ttattaaaaa tcatcaatat caatgtaata acataataaa taataatatc
     6841 gagaaatttg aagaaaacaa agagatttta aatatgtggg tacatgatgt aaagatacct
     6901 atagctataa taaaactcat tcttgaacaa aatgaaaatt taattgatga aaaaatttca
     6961 gatgacatag atagtgaaat atttagaatt gaaaactccg ttgaaaaaat tttatattta
     7021 tcaagactcg aagattttc
//
LOCUS       DAYATN010000171         7027 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.174, whole genome shotgun sequence.
ACCESSION   DAYATN010000171 DAYATN010000000
VERSION     DAYATN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.174"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..1336)
                     /locus_tag="ACGI8A_003371"
     CDS             complement(<1..1336)
                     /locus_tag="ACGI8A_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFH0181335.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIK"
     gene            complement(1347..2567)
                     /locus_tag="ACGI8A_003372"
     CDS             complement(1347..2567)
                     /locus_tag="ACGI8A_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFH0181336.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2581..3369)
                     /locus_tag="ACGI8A_003373"
     CDS             complement(2581..3369)
                     /locus_tag="ACGI8A_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFH0181337.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3386..4660)
                     /gene="larA"
                     /locus_tag="ACGI8A_003374"
     CDS             complement(3386..4660)
                     /gene="larA"
                     /locus_tag="ACGI8A_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HFH0181338.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(4778..5491)
                     /locus_tag="ACGI8A_003375"
     CDS             complement(4778..5491)
                     /locus_tag="ACGI8A_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFH0181339.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5616..6875
                     /locus_tag="ACGI8A_003376"
     CDS             5616..6875
                     /locus_tag="ACGI8A_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HFH0181340.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
BASE COUNT         2101 a         1287 c          803 g         2836 t
ORIGIN      
        1 ttttaattaa catcatataa gcatctgaat ttgattcatg taagtactct tttttagcat
       61 atcctatgcc atgttcacca gaaacttgac cactaagttc tctagatttt ttatacatat
      121 agtcaaaagt ttcttttaat cttattttcc atgtattatc atccatacca tcttttaaaa
      181 tatatatgtg tagatttcca tctcctgcat gaccaaaact ttttattctt atttttaatt
      241 tgtcttgaag ctcgtgagtg tatcttataa actctgctat cttatctctt ggaacaacga
      301 catcacattc atccatttgt gtcgtagatg cctttatagc ttctaaaaat gcacctctgg
      361 ctgaccatat agaatcgttt ctttcttgtg tatctgatat aaatacatct aatgcaccat
      421 tttctaaaca taaattagct actttttcat attctttttc tatatcttca gtgctatttc
      481 catcaaatgt aagcaaaaga taagcatctg atgtattatc tggaaacttt ttacctaaaa
      541 attcttctgc tgccaatatc acatctcttt ccataaattc tatagccgtt ggtattgatt
      601 ttgattttat tattttagga acagtttcta ttgccattga taaatctgga aatggtataa
      661 gcaaacttat acttttcttt ggtaaaggaa gcaattttaa tatagcttta gtaactattc
      721 ctaaagtacc ttctgagccc actaataaat cctttatact atatccagaa ctatttttaa
      781 ccacctttcc acctacattt ataatttctc catttggtag tacaacttca agtcctctta
      841 cataatccct tgtaacgcca tattttactg ctctcatacc acctgcatta gtgtttatat
      901 tacctgcaat tgtagcactt ttttcacctg ggtcaggtgg gtaaaataaa tctctatctt
      961 gaacatattg acctattgtc atcaataaaa cacctggttc tactgtcaat gttaaatttt
     1021 cataatcaac ttcaagtatt ttattcattt tacatagatt aatcattatt ccaccattta
     1081 tggcaacagc agcacctaca agaccagtac cttgacctct tggtgtgact ggtatattat
     1141 ttttatatgc gtatttcatt attttagata cttgttcagt ttcaagtaca tttactaaaa
     1201 cttcagggta cttctcaatc cctccaagtt catcatgtga aaaatcttca tttatatcac
     1261 ttccaactaa tacattttct tctccacaag tatctattaa aaaatctata tcttttttat
     1321 cgattaattt atacatttct atccccctat ttagctgctg atgccatata tttttgatta
     1381 ttatctttgt tattttctat tttttcaatc aatttaggaa taatatcata aatatcacct
     1441 attaaagcta aatgtgctac atcaagtata ggcgctttat catctttatt tattgctact
     1501 atatagtcag caccctgcat ccctgcaaca aattgaactg ctccagatac accacaagtt
     1561 attatcaatt taggttttac agttctacca ctaagaccta tttgtttttt agcatctatc
     1621 caacctgctt ctattacagg tcttgttcct gccacttgag cgtttaactt atcagctaat
     1681 ttatatatca tctccatatc ttcttgtttt tttattgctc tacttgccac aactatcact
     1741 tcagcttctt ccagaccaac ttctttattt ttttctttta cactcagtac cttaatttta
     1801 gattttagtt cttttttttc tactttacat aaagttatct ttcctgtagt attctctatt
     1861 ttttctggtg ctgaaaatat tttatatctt acagtggcaa attgtggtct attattaggt
     1921 gtgtttatat gagccattat atttccacca aatgctggtc ttatttggtc taaatcagta
     1981 tttgattgaa tatcaagtat tgtacaatct gctgtaagac ctgttctaaa tctagcggca
     2041 agtcttggag ctaatgacct acctaatgta gttccaccaa ctaatactat cgttggtttt
     2101 attttatcta taaagttttc tattgctttt gaatatggtt ctatcctaaa atctttaaat
     2161 tcatcttcat catatacaaa tacttcatcc accccataag aaatcaaatt actgcactta
     2221 tcttttatgt cttttcctac aaatacacaa tacacaggtt gttttatttt tgatgccatc
     2281 tcacgagctt tacctataag ctcgtacgtt acaggatgta tatttccatt atgatgttca
     2341 acatatactg caattcccct ccattcatct ttattaattt gtactttact actctctata
     2401 aattcaaatg cgccttttgg tccacttttg acacacaact tgcacatctt acagccagca
     2461 cttatttcta ctatacctga cttttcttct atagccccaa aaggacatat ttctaaaatt
     2521 ctctttaagt cagttatttt atcttgattt acaactatct ttgacattta ttactccccc
     2581 ttacaccaac tttaattcct ctaatttatt tacaagtgca aaacttagtt catctgcatt
     2641 tcctcttaca atatttttat ctgtatttgg ctttggagga aatattctta caacttgagt
     2701 aggagaacca ttaagtccat agttattttc atctttatct tcaaagtcat ctaagctata
     2761 tacaggtatt tctctatctt tagtattaag ttttaattta aatgaaggta gtcttggttg
     2821 aaaaatgtct ttttctacag ttattaaaca tggatattca atctcacata cttgtaaatc
     2881 atttggcata tctatttcaa caactatact ttcatcttta acttcaatta attttgtaac
     2941 gttagtaaca tgaggtatat ctaaaaattc agctacttca ggtcccacct gtgctgtatc
     3001 accatcagta gtttgttttc cacaaattat taggtctacc tttcccatct tcttaatacc
     3061 ttgtgatatg gtataggaag tagccaatac atctgctcct ccaaatctcc tatcacttat
     3121 taatgctcct tcatcagctc ccatcataaa actttcttca attaccttct ttgcctgagg
     3181 aggacccatg ctaagtactt ttagattcgc tttcttatct tcctttattc taagagctgt
     3241 ttctaaagca tataggtcat agggattcat ttttgaatct actccatctc tttttagtac
     3301 tcctgtagtt tcatcaactt ctactgaagt cgttccagga acttgtttaa tacatgtcaa
     3361 aatttccata aagtacactc cttttttatt taactataac tgatactcca tcaggtataa
     3421 ctactacttt gctctcctta ccaactatac tattcgccat ttctaaagct tctttaaagt
     3481 taaatgcatg tttcatatgc atttttttta tcatttcagg attacactta tctgttacca
     3541 ttatcacagt atatttagat aatattctcg ctagtatctg aaattcccat tggtctggaa
     3601 tcgtatctac tctatcgaca cttctaattt tatctaaaag ttgttttgga gaatctgcat
     3661 ttgctacatt ttcataaaaa gattgacctc catgaccatc attacatgct gaaatcatta
     3721 ttattactcc accgtcttta caagtagctt cagctgctgt cattccttta acagattgat
     3781 aaatattttg gtctagtgga tatccaccat ttgtagttac aacaatatca gattttatag
     3841 aatttacttt tgataactct gttacaaatc tacatccttt agtatgtgct ttttcgacat
     3901 caccagcaaa ggaagcaatt atttttttat ctttatctat tacaacattc aatatgaatg
     3961 ctaattttgc aactttagct gcatatacca tatcctcatg aataggatta ttattcaatt
     4021 ttccagttct tgaattatca ctatctataa agtcactaca atgattgtac attatagtct
     4081 ttgcagatgc gattcctgga agtacacttt ttctgccacc tgaaaaacct gcaaagaaat
     4141 gaggttctat aaaaccttct gctatcaaaa gttctgcctc atatgctagt ttgtttatat
     4201 ataaatctcc acctgaaggc aatatcccta ctttacacat agatttatca tcagtagata
     4261 tatgcataac tatatcttca ttatctacta tttcttctcc cattttataa attagctctt
     4321 cccttgtact tggcctatga aatccagttg ccactataat cttcacatca atttctggat
     4381 taacattcct aatccttctt aatataattg gcatagttat tctacttggt acaggtcttg
     4441 tatggtcact tgtaattatg actacattct ttttcccctt aaccagttct tccagagaat
     4501 tagagcctat aggattattc atagattctt caactatctc ttcttgagtc ttatgttcta
     4561 tataattatc cgattttgac tccaaaacac cgagaaaacg ttcatcaggt atttctaaaa
     4621 tcattccttg ttttgaatac ggtactttta actttaccat ttttaatctc ccattataat
     4681 aaactataaa attggtatgt ggtcatacca gttttacatt tatatgatag taccattatc
     4741 taccatttgc aagaataatt tattttataa caaactttta attttcattt tttctataat
     4801 ttttacttat aaaatccata tgttcctttg ctttctcaca tgcaagttct acatcatttt
     4861 ctacaactgc attgtatata gcttcatgct gtttaagcaa tatattttca ttaaaaggtt
     4921 ctgcgattat tttttctctt gcatcttcta taaatccatc aaataatttt gatgctgtca
     4981 taaaaagact ttgaattaaa taatttttac ttaatgttgc tagtttactg tgaaattttt
     5041 ggtctaataa caccagtact cgattattat cttttttatt agtttctacc ctcatttcat
     5101 ctattatatg tttcaattct ataacttcag aagaagttat tctctttgaa gcaagttttg
     5161 caatctcaat ttctaggatt attctaagct ctaatatatc ttcaagtgta ccattattaa
     5221 gtttaaaaat catagataaa ggttctatta aagttttttc tatatttgta catataaaat
     5281 ttccttcacc ctgtctgctt tctatcactc ccatagtctc taatacccta atagcttccc
     5341 ttatggaagt tctactcaca ttcatttgtt cagacagttc tctttctgat agtaatttat
     5401 cccccttttt taactctcca tttagtattt tatattgtat ttgctcaatt acttgttcat
     5461 aaactttttt actagctata ctattaaaca ttttttcctc caatatgata atcgttgttg
     5521 taatatatat ctattatata tcttaagcca ttacattcat atatttttat tacttttaat
     5581 ataatatctt ataactcaaa tttacaggtg atattatgaa aaaaaataaa attattgctt
     5641 tttttgctcc atctatcata gttggagttc tagttttagg gattatccta tacccaaatg
     5701 aagctataaa atctgcaaga gaaggctttt ctatatggtc taatgtttta gttccatcac
     5761 tactaccatt tataattgga gcaaacttaa ttgttgattt aaaaatagtg gatattatag
     5821 ggtttatcat caatcccata actagatttg tatttaatgt ttcaggaaag agcgctttag
     5881 cttttgctat atctacagta tctggttatc cagtaggagt aagtttagct tctgaattta
     5941 ggagtaatgg acaaatttct aagtatgaag cacaaagact tgtgtctttt tgttctacat
     6001 caggaccttt attcataata ggttcggttg gtacaggtat gttccaaaat tcaaacttag
     6061 gttatttaat gattctctgc cattatctag gaactatatt ggttggtctt ttctttagaa
     6121 attatggtaa tgaaaatctt ccaaagacaa aattggattt aaaaacaaat ataaaaaatg
     6181 taatcaacat tagaaattca agtgggaatg gtttttttgt tttatttgga aatgctgtat
     6241 ttaatggagt aaatacatta cttacagttg gtggatttgt aattgttttt tcagtagtat
     6301 ttaaaatact atctcttttt aatgtaatct cattaatagc atcagttata tatttgcctc
     6361 tttcattttt aggtgttagt aaagaactct gtcaagcatt tgtaagtggt ctatttgaaa
     6421 ttactatagg atgcaataaa gtatctagta taacaagttc tccagaaatt ttaagagcat
     6481 ctttagctag ttttttgata ggatttagtg gattatccat cctagctcaa tgttgtacat
     6541 ttctatcaaa aacggacata gatttaaaat tatacatact aagtaaattt gcgcatggag
     6601 ttttagctgc tatctttata tttatacttt atccaattgc aaactctgct gtgttggttt
     6661 ctagctttgc tgatacatac aacgttatat ataataattt aatctggttt tattatctta
     6721 gctattatga tacaattctt caaattgtag taataattta tctaatctca gccgtattta
     6781 ttgcaaaaaa acatatgaaa actttatcta caaatgggaa actaatgcga tataaaaata
     6841 cttttttcac taagaaattt tcacgaaaaa aataaaacta aaaataaaac taataaaact
     6901 taactaaata cttccatttt tttattatat ttgtcataat tttaataacc tttttaatta
     6961 gtttaacagc atagttattt catcacaatt taataatttt attagaaata tttttcaaaa
     7021 aattctc
//
LOCUS       DAYATN010000172         6926 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.175, whole genome shotgun sequence.
ACCESSION   DAYATN010000172 DAYATN010000000
VERSION     DAYATN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.175"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..793)
                     /locus_tag="ACGI8A_003377"
     CDS             complement(<1..793)
                     /locus_tag="ACGI8A_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0181341.1"
                     /translation="MDFRKFAYNNIVKNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNK"
     gene            complement(793..1539)
                     /locus_tag="ACGI8A_003378"
     CDS             complement(793..1539)
                     /locus_tag="ACGI8A_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181342.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            complement(1686..3905)
                     /locus_tag="ACGI8A_003379"
     CDS             complement(1686..3905)
                     /locus_tag="ACGI8A_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0181343.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            complement(4271..5665)
                     /gene="gltA"
                     /locus_tag="ACGI8A_003380"
     CDS             complement(4271..5665)
                     /gene="gltA"
                     /locus_tag="ACGI8A_003380"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HFH0181344.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            complement(5665..6558)
                     /locus_tag="ACGI8A_003381"
     CDS             complement(5665..6558)
                     /locus_tag="ACGI8A_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HFH0181345.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
BASE COUNT         2297 a         1257 c          658 g         2714 t
ORIGIN      
        1 ctttgttctt ataaaatttt tctttatttt ttaaatactt taatatcaaa atagtaaatt
       61 gagaaaaaaa taaaaatatc cccaaagtaa ttaatagtgc tgttgtatac atacctccat
      121 ttatttttaa cttattgcca aatagtatca tagcatatcc taatataaga catattacac
      181 caattattcc aaatagcttt gaagatttta tctctttttt aggctttttt gtacctacta
      241 aaagctctaa aatgctcttt ttactcaata attttaagct cattggtcct gttatcaaaa
      301 ataaaatcat aaatgataca atagtcaata gcattgcttt tactggaaaa taaaattcag
      361 ttaaattcat ataaaacagt ttgcttaaat acaataaaaa caattttgaa attgataaac
      421 caagtaatat tcctaacatc actgctaaaa atcctattat caaattttca attaatatca
      481 attttttcag ttgtctgtta ctcattccaa ttatcatcaa tactccaaaa tctttcaatc
      541 tatatttcaa gaaatttcct aaagagtaca atataaaaag tagactaaaa ccgaatatta
      601 ttaattctga agtcataaga gccataaatg cagtagaacc actctgcatt ccagattttg
      661 ttataattgg atggaacata gacactgcaa aaacaaaaaa tatcattata gaaaaaacag
      721 aacttaaaaa atatgctaaa tatgccctga aatttttaac tatattatta taagcaaact
      781 ttctaaaatc cactatctta cacctcctat ttgagacaaa acagctagta tatcagaata
      841 gaaagaatta ctgctttcag atttttttat ttctgtatat atacaaccat cttttataaa
      901 aattactcta tctgaataac ttgcactata tgcttcatga gttaccataa gtgttgttac
      961 cttttgttct ttgtttatcc tagtaaataa tttcaaaaca tcatcagtag attttgaatc
     1021 aagatttcca gttggctcat ctgcaagaag tatggcaggt ttatttataa tagctctagc
     1081 aatagcacac ctttgagctt gacctcctga aatttcatag gtttttctat ctaaaatttt
     1141 atctattcct aaaaatttag atatactttt agtttgtata ttcatatctt tgatacttgc
     1201 cccctctagt gtaagtggca acataatatt ttcgccaaca gttaaagtgt caatcaaatt
     1261 aaaatcctga aatacaaaac caagttgttt ccttctaaat tcagcaagtt tttctccttt
     1321 caatttactt acatcatatc catctaatac aacctcacct tcactctgtc tatcaactgt
     1381 agatattata tttaaaagag tagatttacc acttccagat ggtcccataa cagacacaaa
     1441 ttctcccttt tctatattta gactaacctt atctaatgct ttaaatggaa tatttccgca
     1501 atatgttttt gatatatttc taatactcaa tatgttcatt ttatattctc cataattttt
     1561 tatttttact acaataccat aatatcgtga atatactact ttaaccataa aaatacccta
     1621 acaattaact tacatatttg taagtaagtt gttagggtat attatagtct aataattatt
     1681 aatgattaat caaatttaaa gtttaagtgt ttttcaaatt catctaatac cataggttta
     1741 gcaaataagt atccttgaac cttatcacaa cctatctctt tcaagaactc tacttgttca
     1801 taagtttcta cgccctcaca gattacttta atatcaatcg cttttgccat ttccacaatc
     1861 ttagaaataa taatttttcc acgttttgta tctgttgttt cgattaaaaa tcctctatct
     1921 aattttaaaa tatcaaaaga aaggtctttt aacagattta aagatgaata tcccgaacca
     1981 aaatcatcca ttgatataag gaatcctatc tctttaatct ttttcattat atcaatcaat
     2041 atatttaaat tatcaaacac aacactttct gttagttcaa gttcaataaa ctcaggagat
     2101 atattgtatt tacttatcaa atcagttata ttttctataa aattatccct atataaatga
     2161 actctagaca tatttacaga gattggtaca ggagtatacc ctttatttat ccacttgttt
     2221 aatgttttgc atacttcttc aaacacgaac atatcaatat ttacaataaa accattcttt
     2281 tcaaataaag gtacaaaatc tataggtgaa ataaacccaa attctggact attccatctt
     2341 atcaaggctt ctgctccttc aatttcattt acatcatcta aactatactt aggttgataa
     2401 tatactataa attcaccttt ttctaaagct ttattcatat tatcttccaa tcgtttttcc
     2461 ttgtacaatc gatttcttgt atcttcatta aaaaatgaat atgaatgttc atatttttcc
     2521 tttttacttt ttgctgcaat atttgctcta tcaattaaca agtcaatctt tttaatatca
     2581 tcctttttag taatcttata tattccactt gacataacaa ttttatactt agatgagtta
     2641 gtagtattta agttacaaat ttctttattt attttagata atctatttaa aagttcatct
     2701 tcagtgttac aagcaatcaa caatataaaa tggtcattat ccatcctagc acaaatttca
     2761 tatttatttg tattattatt taaaacctgt gatatatgaa ttaatatctt attcccttca
     2821 gcatatccaa acatctcatt tatatattta aatttattga catccaattg tactaagtaa
     2881 aaattatctt gataataatt ctttaaaata tacatagaat cttttctaaa cttttcttta
     2941 ttttttattc cacttaaaga atcataataa gccaattttt taagttgttc tattaattta
     3001 gcattactta atttccacaa tatcataaat attactatat aaaaatacaa ctttatcata
     3061 aaattaacta ttagattttt aaagttctta aatatattac attttgatac taaagtccaa
     3121 ttagtccact ttatgttcgt atattttaca ttatataatc tccaattatc ccaagataaa
     3181 aaatcacctt gacatttttt atttatattt tttaagattt cacttttagt ggttcctata
     3241 atattccctt ctaaatcaag catcttactt ttatcattta catatttttt gtttggatga
     3301 gaaataatag tataatcttt atctatcatt agaaatttaa tagaatcatc aaaattgctt
     3361 ctacttatta aattatttac atcttgaaaa taaaaagaag caaaaatggt cccaatcatc
     3421 tcattatttt ttacaattgg tacatatatg ataaatactt tgtcacctgt tacattactg
     3481 acttcaatat ctgaaactac tacttgcttt gttttaattg ctttctcaaa actaggctta
     3541 tcttttattg agttaacatt ttccctatat gtacttgaag catttccttt tgtatctgtt
     3601 ataccaatca taaaaagatt atattccttt tgatatggac ttaataattt tcccttttca
     3661 attagtgatg tttcaccatt agaaaatcta tcatcttgac taagagcttt taataaagat
     3721 atgtttactt ctaaatagtt ttcaagttct ctttttgatg cctcaatcat atttctagaa
     3781 gtaatttcca tagagttact tgcatatatg atggtacttg ttaaatcaat caaaactaat
     3841 agtattaata agattaatat atgcttaaaa actagcccat caaatacttt ttctcttttt
     3901 ttcatattaa aataccatta attacacaat ttatttttct attatttaat gttagatttg
     3961 acaaatctga ttttattagc cacacattat tcactcctta caagaccttt attgtataac
     4021 tattttatca tagttagaca aagttatgtt acttagtatc aaatattaca ataataactg
     4081 ataaattatt aaaccatcta aaaaagtggc tacatcaaaa tagagattgt actaagacct
     4141 aaaagttaga ctttctacag gaatgaaatt ttcattgttg aaatgattaa aacatatttg
     4201 attttgtaca aaaagagtgc ctcatgaact aaaaagttca ttttgagaca ccctctcttt
     4261 aatttacaaa ttaagcatta acttttgctt ttaaatattc atctattgat gctgctgctg
     4321 ttttaccagc acccattgca gatattactg ttgctgctcc agtaacagca tctccacctg
     4381 caaatacacc ctctttagaa gtctgaccat tttcatctgt tattaagcat ctcttcttat
     4441 ttatatcaag actctttgtt gtagaagata ttagtggatt tgggcttgtt ccaagtgaca
     4501 ttataactgt gtctacatca agtacaaatt cagaaccttc tatttctata ggtcttcttc
     4561 ttccagaatc atctggctct cctaattcca ttcttataca aaccatacct ttaacatatc
     4621 cattttcatc tactaaaatt tccttaggat ttgttaatgt attaaatata atgccttctt
     4681 cttttgcatg atgaacttct tcagctcttg ctggaagctc tttttcactt cttctataaa
     4741 ctatatatga ttctgaacct aatcttaatg cagttctagc agcgtccata gcaacatttc
     4801 caccacctac tacagcaact tttttaccag aactaatagg tgtatcataa tcatctctat
     4861 aagccttcat taagtttact ctagttaaaa actcatttgc agaaaatact ccatttgcat
     4921 tttcccctgg tatattcata aacattggaa gaccagctcc tgaacctatg aatatagctt
     4981 caaatccttc atcttctata agttcatcta ctgtaattgt tttaccaaca attacattag
     5041 tttctatttt tacacctaat tttcttatat tgtctatttc aggttgaaca acagcttgtt
     5101 ttggaagtct aaactcaggt attccgtaag ttaaaactcc acctggctca tgcattgctt
     5161 caaagattgt tacatcatag ccttttttag ctaaatcacc agcacaagct agacccgctg
     5221 gaccacttcc tattactgct actttttgat ttttcttagg ctctgtatca gataaattta
     5281 tgtcattttc ttttgaccaa tctgctacaa atctttctaa tttacctatt gatacagcat
     5341 ctgattttat tccaagtata catacacctt cacattgact ttcttgtggg catactctac
     5401 cacaaacagc tggtaatgaa ctgctcttag ctattacttt agcagcacct tctatatcat
     5461 cttctttaat ttttgttata aaccctggta tgtcgattcc tactggacat ccacccacac
     5521 attttggttt tttacaagct aaacatctat tagcttctgc catagcttct tctttattat
     5581 accctaagca tacttcatca aaatttgttg ctctcacagc aggttcttgt tctctaactg
     5641 gtacttttac ttttttagca tccattattt gttacctcca catccacaat tactatgatg
     5701 atgactatcg ccttcttcaa gttttagcat agctcttccc tcttgagtct tgtacataga
     5761 ctgtcttctc attgcttgat caaaatcaac taaatgacca tcaaattcag gaccatctac
     5821 acatgcaaac ttagtttcat ttccaacact aactctacaa ccaccacaca tacctgttcc
     5881 atctatcatt attgtattta aacttactat agtaggtata tttagttctt tagttaattg
     5941 gcacataaat ttcatcatta tcattggtcc aattactatt gcttggtcgt attttttacc
     6001 ttgattatca actaaatctt ttaataggtc tgtaactctt ccattaaatc catatgtacc
     6061 atcatctgtt gatacatata catttttagc tacttttttc atatcatctt ctaatataat
     6121 taattcttta gttcttgcac caattattac atctacatct aatccatgct ctttaaacca
     6181 tttaacttgt ggataaactg gagcagaacc aactccacca gctacaaata atatcttttt
     6241 atttcttaat tcttctatat cttcatgtat aaattcactt gcttgtccta atggccctac
     6301 aaaatccatt acaaaatcgt tttcttcaaa cattgctaac tttttagttg aagcaccaac
     6361 tgtttggaat acaatagtta ctgttccttt ttctctatca tagtcagcta ttgtaagagg
     6421 aatcctctct cctttttcat catttttaat tattataaat tgacctggtt gagaagattt
     6481 tgcaactcta ggtgcctcta tttccattaa ataaatacta tctgttaatt gtctcttact
     6541 aactatttta ttactcatct cgctattctc cattctaaca aatatatttt tcccacttta
     6601 aatttaatta aaaacataaa cacacaaaac tataactctt tataatataa tattagttct
     6661 tggttgattg ttttgtattg gacatccccc ctataataat tataactgcc caaactacaa
     6721 atagttgata ttaacaaatt tcttgtaaat gccaataaac actaagtcta tatcatacgt
     6781 ctaatttgtt atagatttaa cctaatacac acttaaagta tactatatta tgccaaattt
     6841 gtaacaaaaa ttttataaat acataataaa atttttttat tcaattattt atttaataaa
     6901 ttaacttagg ttttcataat aacact
//
LOCUS       DAYATN010000173         6880 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.176, whole genome shotgun sequence.
ACCESSION   DAYATN010000173 DAYATN010000000
VERSION     DAYATN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.176"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            192..1352
                     /locus_tag="ACGI8A_003382"
     CDS             192..1352
                     /locus_tag="ACGI8A_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFH0181346.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1694..1855
                     /locus_tag="ACGI8A_003383"
     CDS             1694..1855
                     /locus_tag="ACGI8A_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181347.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2048..2677
                     /locus_tag="ACGI8A_003384"
     CDS             2048..2677
                     /locus_tag="ACGI8A_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFH0181348.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2916..4175
                     /locus_tag="ACGI8A_003385"
     CDS             2916..4175
                     /locus_tag="ACGI8A_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFH0181349.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4446..5429
                     /locus_tag="ACGI8A_003386"
     CDS             4446..5429
                     /locus_tag="ACGI8A_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFH0181350.1"
                     /translation="MTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIKVIKH
                     NNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGIGLDE
                     LILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDFGITK
                     ANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNSEEKM
                     LQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKAYIEV
                     EDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5675..6742
                     /locus_tag="ACGI8A_003387"
     CDS             5675..6742
                     /locus_tag="ACGI8A_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFH0181351.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFAEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
BASE COUNT         2721 a          674 c         1183 g         2302 t
ORIGIN      
        1 ttcagattat tcatttctaa agtaattaat gtgcaaatct ttattaatat atgtcaatag
       61 ttaggtatat atgtatttaa ttagcatgat atagataaaa gtatttattt taattgtcta
      121 ttatctgaaa aagtctttac ttagaaatca taaaaatcaa attaattagg aaaagaaagg
      181 gtgtgtataa tgtgattgag actggatttg tatattgtag tttcttggtt gtattcgtag
      241 gtgtaatttt ttttatggtt gataaattga aatggaaggt atttgatgtt attaatccaa
      301 tgcttttaat atacttaggt tcagctatac ttggatcatt tggtttattt gcacagaatg
      361 aagatgttgg aacgtatcaa aacataataa cagtgaactt ccttccatta atgttagtgt
      421 ttttgttaat tcaatgtgat ttaagaaaaa tattaaagat ggggcctaaa atgttattat
      481 catttttctg tgctacattt acatttatgc ttggatttgt tatagcattc ataattttta
      541 aaaattcaat gggtgcagat tcatggaaaa ttcttggggc agcatctggt tcatggatag
      601 gtggctcatc aaatatgatt gcagcagcta atgctctagg agttgcagaa gaaggactta
      661 gttatgcaat acttatgggt tctataggat atacagtatg gatgtctgtt tgtctaatgt
      721 ctgtgaaatt tggacctaaa tttaacaaat ggacaaagtg cgatacaagt tttatagatg
      781 aggttacatc aaaacttgaa aaagaaaata aagataatac agcacctacg tttgtagaac
      841 ttatgatttt actttctgtt gggtttggag ttgcgtcttt tgcacaaatt gtatccaaga
      901 tacttccaac ttcaggtata cttaatgaaa ctatgtgggt agtacttgtt gcatctacag
      961 tatcaatatt attgggtatg acaaaaatat caaagcttaa agggagtaca ctggtaggaa
     1021 atgtgtttat gtatttactc cttgcagcta caggtagtaa agcaaatttc tttggattgt
     1081 cagaagcacc tgtttacata atgtttggat tggtagtagt ccttgtacat gctatttcat
     1141 ttatagggct tgctaaacta tttaaacttg atttatttac ttgtgaagta ggaagtatag
     1201 ctaatatagg aggagtaact acagctccaa tgattgctgg ggttcacaat cctgtattaa
     1261 taccagtagg tgtacttatg gggcttctag gaaatattat agggacttat tgtgctctta
     1321 tggtaactca aatattacat atgttagctt aataaaaata tggtataaaa ttaataattt
     1381 aaataagaaa agtcatttca atttaagcag aagcttttac tgaaatggct tttttgtttg
     1441 acaagataat ctatttcaag aagatgtttt gacatattta gtgtataata ttgtaaaatt
     1501 tactaaaaac gagatatatt taatttttat attgaatcga acgtctgtat gatgtataat
     1561 aaatatacag aacgaatgtt tgcagaacgg gtgttttttg aatttatttt aataatttat
     1621 gaatatatat cagaatgcat aaatatattt aaatataaag attaaaaaga atttaaagga
     1681 gtgttgataa cttatgataa ttccatcaag aagaatacaa gatataacat taacaaattt
     1741 aaaaaatggt gaagtaactt taattgaatt ggatgaaata tacaagaaga tgggattttt
     1801 atttgttgtt agtgaaggca aattgaaaaa gataaaaaag gagaatcatc attagaaatg
     1861 aatgaaataa taaatattat aatagtttat aaaacctaac ttatgtagaa tataaatact
     1921 taagttaggt ttttttgtga taaagtttat aagtactcaa ttttataaga taaataatgg
     1981 tcttaagtga aaagtaaata agtttaaatt ttagtaagta ttatattgca taagaggtga
     2041 tattattatg aaaatagtaa cctacaatat tcataaaggt atggattcaa ataatagatt
     2101 aactctaact aaaatggggt tatatttaaa acaattggat tgtgatgtaa tttgtttgca
     2161 agaagtatta tatcctcaat ttttagcttt aaaagctgtt ttaaatatgg atggtgtctt
     2221 tgcgacaaat gtaaaaaaag taaatatgat atatggtatt tgtacattta caacatctaa
     2281 aatgttaaac agtaatcatt ttttcttaac tagcaaaaaa gaacaacggg gagctctttg
     2341 tataactata gatgcatatg gaagaattat aaatgtaata aatactcact tgggacttga
     2401 taggcaggag agagcaaaac aattagacga aataatagac tataggaaca gattagtggg
     2461 gatagttatt ttatgtggtg attttaatga gaaaaatgtc tttttaagta tgtttaatga
     2521 tatggctatt tcactaaaca agtcttactt accaacattt gaaaaatcaa actctagaat
     2581 agattacatt tttgttaata aaaatactga actaaaagga tacacagtag aaaaaattta
     2641 cctgtcagac cattatcctg taataggtta catttaaatt tcaatataaa tgaaattagt
     2701 tgttataaaa taaacaattt tttgttaaga aatagtaaaa gaaatataat aaaaaatatc
     2761 aaaaataaaa ctaattataa gacataaatt tacaataaag ttacatttga ttaaattcct
     2821 tgtttaacaa aagctattca aatataatct aaggtagttt tgaaatggga actctattat
     2881 gcaaatttaa gctagtgtta aaatgaggtg aagttatggg cagaagaaca acaagagttt
     2941 caagattagg taaagaaaaa aaacgaagtg ttttaattgt attggcagct ttagttctaa
     3001 taatgggtgg atttacttat ttttttaaca gtaaatttct gtacaatggg aaaatcgcta
     3061 aaaatgtata tatagaagga gtaaatgtat ctgatatgac taaagcagag gcattaaaag
     3121 ctataacaga taagtatact ccagaagatt taaatcttgc ttatgatggg aaaaaatata
     3181 caatatcacc aaaagatatt gatttaaaat atgatacaga agatgttgta aaagatgctt
     3241 atgaaagtac aaaaaaaggg tcctattttc aaaacttaaa aaaatatatt gatataagag
     3301 tgaataaggc aaatatgaag ataaaagctg aatatgatga ggcaaaactt agttccaaag
     3361 tgtcttctat agcagatagt ataaatgtta agatgaaaaa tgcttctatt agtgttggaa
     3421 gtggtggact aagttataca gactctgttg taggtagaga atttgatttg gcagcaaata
     3481 aagagtctat atataatatg ataaaaaata aagagcataa aactttagaa cttaaagtaa
     3541 atcttcaaaa accagatata acaactgaac aagtaaaaac tgtaaattct gttataggtc
     3601 aatattcaac tacttattca caagctgtag agggtaggtc atataatgta ggattatcag
     3661 caagaaaaac tagtgatgta ttattaatgc caggtgaaga attttcatac aataaactta
     3721 caggacctag taataaagca aatggttaca aagatgcacc tgttatagtt tatggtaagc
     3781 tagaacaatc agcaggagga ggtgtttgtc aaacatcttc aacagtttat aatgcggctc
     3841 ttctttcggg tatggagata actcaagtta caaatcattc atctgcatct acttatgtgc
     3901 ctaaagggag agatgctact gtgagcgatg gaggattaaa cttaaagttt aaaaatccat
     3961 ataagcatcc tgtatatatt aaaaattatg caggtggagg aagtgtatca tcagtaatct
     4021 atggtaattc tggtgacaaa ccaaatatat ctatagaggt aaaacaaact ggtcagaata
     4081 aatattctac atatcgaata tttaaagatt caagtggaaa ggtcataaaa aaagaacata
     4141 tatcaaatag ttcttataaa gaattaaaaa aatagataat tttttagaga aatatttgta
     4201 atataaaagg gagtgtctca aaatgaactt tttagtttat gagacactct ttttctgtga
     4261 aatcaaatat attttcatca tttcaacaat acaaaagaag cgtatctcta ttttgagaca
     4321 gccttttttg taaaaattaa cactattagt tcttgtttta atattacaat cattaatata
     4381 tagaaaatat tcctatatat tggtataatt gtaaatagat ttagttatat aaaatgtatg
     4441 aggaggtgac atggttgaat tttataggca atagatatga ggtagtaaat tctgacgacg
     4501 ttttagaaat taacaaaatc tataaagcta gagatgtttt ttacaggaaa aatgtgttaa
     4561 taaaagttat aaagcacaat aattatatct gtgaagattt tgtttcaaat ttaattgatg
     4621 aaagtactgc tttagatgag ataaactctc cttatatact aaaaataata gatgttggga
     4681 ttcactgtac agaagaaaca acattatatt atgttgtaag tgaagacttt aatggtatcg
     4741 gactagatga attaattcta ggtaattatc ttcatttaga agcaattgta aacataatga
     4801 tacaagtttt aaaagcttta gaaatgatac ataggaatca ttcatatcat ggtagtttaa
     4861 aatcgagtag tataatagta gatacagaat acaatataaa aatttgtgat tttggaatta
     4921 caaaagcaaa taatggggtt aatacaagaa gctatggaaa caggagatat ttatgcccac
     4981 atcaactatg tataaattat actgataaag aaagtgattt ttttgcaaca ggtcttattc
     5041 tatttgaatc tatatttaaa aaattgcctt ttggagaatc aaattcagaa gaaaaaatgc
     5101 tacaatctat tgataaggga ttagattgga atagtataaa agcaattaat ggaaatactg
     5161 aacttataaa tgttataaaa aggcttcttg gaagaaataa taagtatact agagccaatg
     5221 atattattat tgatttaagt aaagtaatgt atgaaaaagc atatatagaa gttgaagatg
     5281 agaaagaaga agagcagaaa caagacaaag ttatacaagt taaaaaagaa tataaaagta
     5341 aaatgagaaa aaaattacat aaaaaacttg ttgttgccgg tctagctata gtcatgattt
     5401 caatgattat tattagttcc ataatataaa aattgtatat taagtatttt aaaatcaatg
     5461 tctataaagg atatacatag aaattttagt gtagtcttaa attatgaaag gaagtggtta
     5521 ttatgttaac aaaaaataat gaggtattaa acgtctagga gtattttaac aaatagccac
     5581 ctgcttttac agtttagaat aaaaaagctg tggctaaagg tcacagcttt ttgctacttt
     5641 ttaaaggcag gagaatatta aaaaggagca aattatgagc aatataaaaa taatagcaag
     5701 taataaatgt tggattgaag atatagcaag gaaacaactt gaagatattt caaagttaaa
     5761 tggaatttta agagtagtag gattacctga cttacatgca ggtaaaatac ctgtaggttt
     5821 agcagttgag actaaaaata ttatttatcc acatataatt ggaaatgata taggatgcgg
     5881 aatgacttta tttaaaactg gggttctaaa aaagaaattt aaaaaagaca aatggattaa
     5941 gtcacttagt aagattaaag atttgtctga tatagaaata aaaaacacat ataaagaaga
     6001 atgtccaatt ttaaatttag gaactatagg gagtggaaat cattttgcag aaattcaatg
     6061 tataagtgaa atttataata aagaacaatt tgaacaatta aaattttcat ctgatgatat
     6121 tatgatgtta gttcactgtg ggtcaagaaa ttatggagaa gatattttaa aaaaatttta
     6181 tgataaagat ggacttgaag ttgaaagtga gaaagctata gattatatta aaaaacatga
     6241 taatgcttta atgtgggcag agcgtaatcg tgaagctatc agtaaaaaaa ttatgcagtc
     6301 aataggtaca tctggagatg ttgaaacaat attctctata aatcataatt ttatagagaa
     6361 aagggaagat aaatttatac atagaaaagg tgctgtttca agtgaaagag gagctgtgat
     6421 tataccaggt tcaagaggca gtctatcata tatagttatg cctactgaaa atacagagat
     6481 atctttatat tctctatcac atggagcagg tagaaaatgg tctcgttcag tttgtaaatc
     6541 tcgccttaaa agtaagtata gtaaagatac tataaaacaa actaaattta aaagtcagat
     6601 tatatgtaat gatgtaaatt tattatttca agaagctcca gaggcatata agaatataga
     6661 gcagattatt gaaagtttga tagaatatga acttattcaa gtagtggcaa ctatgaagcc
     6721 attgattact tataaaggat aatttttaaa gttaaaaaag attaaaatta tattataaat
     6781 agctaatttt atggaacatt taaaagtgtt ttattattag ctattttttt attttgtaga
     6841 gacgataatt tatagagcta aattctttga tatataatga
//
LOCUS       DAYATN010000174         6875 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.177, whole genome shotgun sequence.
ACCESSION   DAYATN010000174 DAYATN010000000
VERSION     DAYATN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.177"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..261)
                     /locus_tag="ACGI8A_003388"
     CDS             complement(<1..261)
                     /locus_tag="ACGI8A_003388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HFH0181352.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGA"
     gene            complement(639..1178)
                     /gene="hpt"
                     /locus_tag="ACGI8A_003389"
     CDS             complement(639..1178)
                     /gene="hpt"
                     /locus_tag="ACGI8A_003389"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFH0181353.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(1506..2813)
                     /locus_tag="ACGI8A_003390"
     CDS             complement(1506..2813)
                     /locus_tag="ACGI8A_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HFH0181354.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(3238..5013)
                     /locus_tag="ACGI8A_003391"
     CDS             complement(3238..5013)
                     /locus_tag="ACGI8A_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0181355.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLERMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(5010..5147)
                     /locus_tag="ACGI8A_003392"
     CDS             complement(5010..5147)
                     /locus_tag="ACGI8A_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181356.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(5225..5794)
                     /locus_tag="ACGI8A_003393"
     CDS             complement(5225..5794)
                     /locus_tag="ACGI8A_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFH0181357.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(5978..6613)
                     /locus_tag="ACGI8A_003394"
     CDS             complement(5978..6613)
                     /locus_tag="ACGI8A_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFH0181358.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2147 a         1256 c          661 g         2811 t
ORIGIN      
        1 cgctccagtc gctcctgtat ttcctgttgg tcctgtagag cctgttgttc catttgctcc
       61 agtcgctccc atatttcctg ttggtcccgt tattccattc gccccggttg ctcccgtatt
      121 tcctgttgga cctgttattc catttgctcc ggtcgctcca gttgcacctc taggccccgt
      181 cggtcctgtt ggaccagtag cacctgttgg ccctgttgga cctacatctt gatataaact
      241 tggacctgaa atatcactca tataattaat cctccttttt taaagttaga gtattactct
      301 ataaatattg agtattactc tatataatta tatgaatcga caatttctta tgtgataatg
      361 tatctatttg cttatatcaa catatatgct ttgattcttg aatttcatta cttttttgta
      421 aaatctttta caattattag tactctttta caattattaa tacaattaaa aaactcattt
      481 tccatcaatt atttaaaatt tttttattat gttataaaaa atagataatt aaattgcata
      541 aaattaattt aatactactt taattacttt gtaaataaaa aaacctcaac acgattaata
      601 aaaatcatat taaggcatcc acaaaattat ataattgctt atctatcttc atcagtaaca
      661 ttgaatatat aaggaacatt tctataataa tcaccaaaat caaacccata tccaaccaca
      721 aacttatctt ctatttcaaa tccacaataa tctggcacta aatcgacttt tcttctgctt
      781 ggtttatcca aaagaacaca acattttaaa ctagcaggat ttttagcttt taaatgtttt
      841 aacacaaaat ccatagtaag tgcagaatca gtaatatcgt ctacaaccaa aacatcatat
      901 cctgctatat cagtagtcac atctgaaact actttaaccc ttccagtact ttcttcattg
      961 tttccataac tagatgtagt cataaaatta actctaagtg gtaaatttat atttctaact
     1021 aagtctgcac aaaatataaa gctacctttt aatagagaaa ctaccataag atttttatct
     1081 ttataatctt tttctataat tgctcctaat tcccttaatt tatctttaat tttttcttct
     1141 gaatacaata cttcccattt ttttgtttct atatccataa aaagacctcc aattgtaata
     1201 atccttctta attttataag agattgctca ctttttcaat actcttattc aatattagtc
     1261 aatattattg agtttttatg ttcattcaac aattattaac aatgtatctt atattttaag
     1321 tagttcatat atccacacta aattacatac tatatatttt agacaaaaaa agaaaaaact
     1381 tgcactaaca agttttttct attatacata aatttataca taatcatatt ttattagagg
     1441 gtactaatta aatttattta tgctattttt gttatacctc ataatacaaa acatattata
     1501 aagtactagt caactttatt catatcattc attacattta agattatctc atctgttata
     1561 gacatacctt tgtcacttac tctacctaag ttttgtatac tttgctcaac acttgaacca
     1621 actataccat ttagtggtgg tacaaaacaa tcttgcttag ctattatagc actttgtata
     1681 gctgcagatg ctgcagaaga taattttaat gcacaaccag ctttcgcacc gtcacatatc
     1741 attccactta agtcagctat catattttct atagctcctt ctatttgctt ttcagtacca
     1801 ttcataagcc aagagatacc agcagttgct ccagtagaag cagcaacacc acatccacat
     1861 acagcagata atcttcctgt atagttctta atataaccag ttactagatg agatatagct
     1921 aaagcttttg ctagtctttc atcagattgt gggaattttt tattataagc aacgattgga
     1981 agaattgctg ttaatccatg atttccactt ccatttgaac tcataacagg catttttacc
     2041 cctgccattc ttgcatctga agctccagca gttaacatca ttgcataatt tataacatca
     2101 ttacctaaaa gaccttcttc tatagacttt ttaattccat acccgacacc aataccagtt
     2161 ttttgtttta atccatattc agccatctct tcattcattt ttactccatc tagtaaaaat
     2221 tctatatctt taaaatcaat ttcttctaca ttttttatta attcttgtat agttactgta
     2281 tccattaaac tttcagcttt ttctgccgca tcatttgaag ctgatacttt tggttcattg
     2341 tctaataata cttctccatc tttttctaag aacgtaaaat tatcatgttt agttcttatt
     2401 ctcactttag caatatgatt ttttcctttt aacacaactt ctataaatac tttctctctt
     2461 gtatcagcag gtgttattga tagtggagta ttatccatat aatcctcagc tattttaact
     2521 tcctcttttg ttagagtttc taacacactt aatccatttt cagaatgtcc acctactata
     2581 cctagagctg ctgcaatttt taatcctaat ctttcagtac ctggtatacc tacacacata
     2641 ccatttttat agatactagg actaactaac atacgatttt caactatttc ttctcctaat
     2701 aattcctttg cctttgcaca tgctagagct aaggctactg gttcagtaca ccctactgct
     2761 ggtttaactt ctgcctttaa catttccact aattcttttc ttatatctct cattatatat
     2821 aaacctccca attaagttta gatttagtac tcaaataatt agttaaataa tttcttatct
     2881 atatttatag caaaatacat gccatatttt ttatatcttt ttatattttt agtcttatat
     2941 tctataacca ttggtatgac tgcctctatt tatgtatttt ttttatttgt atagagtatt
     3001 tatattcttt aaaatggaga aacatatgac aatttcgcaa aaagacaaaa aaaagtctca
     3061 aaatgagatt ctatatcatt ttgagacggc tcaaatatat tattttttaa cttataaaaa
     3121 ccatttttta attcataaaa aaagattctg caaaacactt atagtataag tttaaagctt
     3181 tctttaaagt tatgaattta gaaaaattta aattttatat agctttttat tatattacta
     3241 tgaaattata ttgtattttt caatttttct atataatgta gctcttgata ttccaagtgc
     3301 tttagctgtt aattctttat cttttttata atctttatac ttatttaaag cttttattat
     3361 ctcaatcttc tctagttcat caagtggaat tatcctatcg cttttaactt ctaactcttc
     3421 tacagaggta ttcttaattt tttgaggaat tacattcatt gtgattattg aagaagaaga
     3481 catattcaca ctatattcaa taatattctg caactctctt atattccctg gccacttata
     3541 ttccatcata gcatcaacaa ccctgctatc tatgtcaata acatttttat ctaatttttt
     3601 tgaatactct ttaaccatat gcttgattat aagtgggata tcttcttttc tctctcttaa
     3661 acttggtaat tttataggta taacatttaa tctataatat aaatcttctc taaaagtgcc
     3721 attttgaacc attctttcta aatctttatt tgtagcagca ataattctta catctattga
     3781 aacatttgat ttacttccaa ttttattaag ttccttttct tgtaatactc tcaataactt
     3841 tgactgtaaa tgcaaactca tatctcctat ttcatctaaa aatatacttc ccttatgagc
     3901 tacttcaaac attccaagct ttccaccttt tttagctcca gtaaaagctc cctcttcata
     3961 tccaaataat tcactctcta ataaattatc cggtatagca gcacagttta cagctataaa
     4021 aggattatct actctattgc tatggttgtg tatggctctt gcaaatagct ccttacccgt
     4081 tccactttcc ccagtaataa gtactgttga tgttgattta gaagctatta gggcttcctt
     4141 tttagaattt cttatagcta cactttctcc aattatgttt tccaatttta ttctataatc
     4201 tttattcatc ttattgtagt tttttatagc atctctttta tctataaaat caataacata
     4261 tccctttaac tcatttttta atactatttt attgatgttg tatattccct tcgaatcata
     4321 tccgtatctt ttataaaaaa aactacagga tttgtccttc ttaaagttat ttatagaagg
     4381 tttctttata aaatctaact cgacaaatat gtttttgcca gttatactat cttttatatt
     4441 gaaaagctct ttaaattttg aattatattt atcaattttt ccatccacat caatagacac
     4501 aacagctttg tccatattgt ttaatagtgt ctctaatttt ttcttttcta attcaagctc
     4561 ataagtttta atttgtgctt ttaatttatt agatattagg tctgccattt tgcctaaaaa
     4621 gtttataaga ccacttttat tattttctat aatttgtgct tgttcttttg taaaggctat
     4681 aaggcctata acaccatgag aatcgccatc acatataata ggacaacaca cctctgcaaa
     4741 ctctacacaa gccccacttt tatagcattc cctacatata tcactactct ttgggtcatc
     4801 aattagtatt gtaatctgtt ctgtaagtga ttttttaaaa gccgtatatt tatctacttt
     4861 ctcacctatt ttgtctatat atactcctgt acctgcaatc ctaaataact ccttatttac
     4921 tatagttaca tctatattga gtactgatac tatagcttca gctatatttt gaatatcaga
     4981 ttttatatat tctaaaccta tcataatcat cattccttat ttttctttag cataagtatt
     5041 tgtcttattt ctttaagaat caagtatatc ttatttaaag taaattggat tgacatcagg
     5101 attacaaaga gcataacaat tattattaac tcataattta tttccatcta aacatctcct
     5161 tataacaaaa gactaggctt aacctagtct tttatattat atccatattt atattttaac
     5221 tcatttatct tctttttccc acataaatat attttaattt atttgactct ttaatcatac
     5281 catcaaagtt taatcttgaa aatatattaa atacttcatg tggatagtga atctttgcaa
     5341 cagaaccaca aaatttatgt ttaagatagt ttaacttaac ttttaaattt tttaaattta
     5401 aatcctctaa tataacctct ccattgccct ttaacactct ttgtatttca gatgaagctt
     5461 tttcacaatc tgtaataaca ttaaattggt caactattat tatcttgttg aactgattat
     5521 catcaaatgg tagaaactct aacttacctt taactacatt gcaattttca ttatacacta
     5581 gagaataaca gacatcttca taattactat tctccagtat agttacagaa gttccaagac
     5641 ctctcaatct cttgcctagg tttcctatat gaccaacaag caatatttta tcacctacat
     5701 tatactcaat tgtatccaaa aaacattcta attcatcaga tgtatattta gctttaactg
     5761 gctgtttatt tatctgacat aatatgcttt tcacaatgct tcacctcttc atttcctcaa
     5821 ttataaatta tgtacagatt acattataat aaatggcgag caactattca agagatattt
     5881 cattagtatt tttttaaaca atctttatac attttaatat gatattatac caaatttatt
     5941 aggattttat tcataaaaat cagtctattt tttatgttta ttcattgtta ttttcatcca
     6001 atttttttat tttcgatatg gatacttcat atgccatttt tttgaccaca ttattttcat
     6061 ctattttttt ctcatattct ctactttgaa ccctacccca aagctgtatt ctatcaccaa
     6121 cctttagatt ctttgcaaat ttagcatttc taccccaaac tattgatggt atataatcag
     6181 atttattata aggtctgttt atagcaacta ataaatcggt tatttccctt cctaaaggtg
     6241 tcttcctata cacaggttcc ttacatacat atccatctaa aaatatacta ttagggtctt
     6301 tattttcctc gtctatactt tttatttctc tgacaaacac tgtaagcact aatctatttt
     6361 tattatttaa tgttttgtta tatgatctta attggcctac gacattgact aaattttgtt
     6421 tatcaaggtc aatatcttgg agaagtcttt ctgatacagt cattggaagt atatcaaaag
     6481 aatcgcttag tctgtttatt tttattttca tattataaaa tttttctcca aatatctcat
     6541 ggctaaattc tagcttattg tctaactccc ctcttaagtt tactacgtta ttctcatctt
     6601 taacagctat catattttat ccctccacaa ttgttaattc ttatatttct ggtatcagct
     6661 tatataataa atatatttat aagggatgta ttttattaca aatctttgat tgctgtttaa
     6721 aagtttttat ttagttataa atttcattta atatttcact tttataatta taaaattatt
     6781 atctttttgt accacatata ctaattaaac tacctatttc agttttgtat taattataaa
     6841 aatcctatta tataattaat aagtcatata taact
//
LOCUS       DAYATN010000175         6862 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.178, whole genome shotgun sequence.
ACCESSION   DAYATN010000175 DAYATN010000000
VERSION     DAYATN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.178"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            115..255
                     /locus_tag="ACGI8A_003395"
     CDS             115..255
                     /locus_tag="ACGI8A_003395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181359.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(252..623)
                     /locus_tag="ACGI8A_003396"
     CDS             complement(252..623)
                     /locus_tag="ACGI8A_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0181360.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1172..1429
                     /locus_tag="ACGI8A_003397"
     CDS             1172..1429
                     /locus_tag="ACGI8A_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181361.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1539..2219)
                     /locus_tag="ACGI8A_003398"
     CDS             complement(1539..2219)
                     /locus_tag="ACGI8A_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFH0181362.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            2479..2778
                     /locus_tag="ACGI8A_003399"
     CDS             2479..2778
                     /locus_tag="ACGI8A_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181363.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            3102..4757
                     /gene="ilvD"
                     /locus_tag="ACGI8A_003400"
     CDS             3102..4757
                     /gene="ilvD"
                     /locus_tag="ACGI8A_003400"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFH0181364.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            5101..5730
                     /locus_tag="ACGI8A_003401"
     CDS             5101..5730
                     /locus_tag="ACGI8A_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0181365.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            5756..6568
                     /locus_tag="ACGI8A_003402"
     CDS             5756..6568
                     /locus_tag="ACGI8A_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0181366.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         2711 a          761 c         1074 g         2316 t
ORIGIN      
        1 gataatcaaa atatatttta taaaatcaaa atacaattac ataaacttta ttaaaaattt
       61 gttgggaatt tctgactaac attaagaacg taagtttgtt gggggatggt atatgtggaa
      121 aagtcaaaca ctactgttac aaaaaaagaa aaaattgaag atataggaat gaaattagaa
      181 catttattga gtgaagatat tactttttat gaattattac ttgagatttt gtataaagaa
      241 aaagaagaga attaattctc ttctttaagg aatcttttta aataggcatt atcaatcatt
      301 ttttctatag cttctctatc ttctttttca agagaatata ttttttctaa gagctttttt
      361 gttttttcac ttactgataa gttctctatg attaatttat cttctttgct tatttttgta
      421 ttatcattta cgcctaataa ataatctgtt gatgtttcta atgcttttgc taatctaact
      481 attatttcac ttttaggttc tcttaaatta ttttcataac gtgacaagct agcctcagtt
      541 ataccgactt tagtagcaag ttctttttga tttatatcta attcttttcg tagatttgct
      601 attctatctc ctaaactttc catatgtaca tcactcctta ccgaaattat aagttatttt
      661 gataaaaagt aaaataaaat taccaaaaat ataaaaaaaa aaggttaaaa taataaaata
      721 tattgaaaat taccatttgt atatgtataa tatgaattat acaaatggta atttgatgag
      781 aggtggtatg ttgaaataag aaactgaatt aattaataaa tacatagaaa gttttgaaaa
      841 cctttaattg agatataaac aatatgtaca aaattttttg caagtaaaat taccaaatgt
      901 ataaaataaa tcagtagcat agataatttt atgttttaat acattagatt atttaattta
      961 gttataatgg tcaaaaatgc aatctacaaa taaaacaaag tattatcaaa aaagtattct
     1021 ttaagaaaat tttttattag agatgtaatt gatataaata atcgtattaa aaaataaaat
     1081 actaagttta aaaatttaaa taatctataa atcaattata aaatattatt tattagaaat
     1141 taaaatgaat taagtaaatg gaggaattga tatgataata attagaagtc aagataaaac
     1201 taatttaatg cagataaacc aaattaaaat agatggtagt caagtttatg cagtatttga
     1261 aagtaaaatt gatactgtaa aaataggaga ttatgaaaat aatacaagag ctatacaagt
     1321 gttggacaat atacaaaact tcattgaaaa tggaacaaaa tatgattata taacatcaaa
     1381 taaagtcaga tacaatgtta ataaaatatt tcagatgcca gctaaatagt gagtgtgcat
     1441 tttaatttaa taactaaagc ccactttctt ttgtagtatg aaagagggct ttacatattt
     1501 gtaaatatcg aagtacactt attttatcaa taaattattt aataaggtat aatggaccaa
     1561 ttaaacaata aaaattctcc taaaacagca agtattaaat atataatagc ttcatttttg
     1621 taaagagagg taattttttt gcttatgtca tcatcaattg taatagcaat ctcatcttga
     1681 ataatatttt tatatataga tgggaaaata tcttttttct ttataagatt aaataaatta
     1741 tttacaccta cccatataaa atatcctaaa acagcaccta atgtgttcat cattaaatca
     1801 tcaatatctg tagctcttaa gttaaacaat tgacaaagtt caattgctaa tgaaaaacct
     1861 aatccagtta atgaaacatt gataatattt ctaaaatttt tccaaattaa aggtagtaaa
     1921 aatcctaaag gcataaacat aattatattt aaaatatatg ttaagatgcc aactgagtca
     1981 aaaggtatta gatttatttc atcaattcta aaaagcccac tataccttcc tatatcccat
     2041 atgctaccaa ttccagcttt actaagtgct agatagatat ataataaaaa aatatatacc
     2101 catactaaat gtatcattac attcttttca actttcaaat tttttcgttt tataacgact
     2161 atttggtata ttaagcatgg taatataacg caaataaatg aataaaatgt ccatatcatt
     2221 ttattttgtc ccctttcaaa tttatgtttt tagttttata agtactatct ttatattaaa
     2281 ctgtaagttt taaaaatctt ttaataaaat cttaaaaatt tattaattta taaaaatata
     2341 ttattaagat taaaaaatat atataatcaa agtttctaca attatatagt tagggatata
     2401 aaaattttat cgtatgcata aatattataa atatatggaa ataataatag tatattttgg
     2461 taaagtagga ggaattttat gaaatggatt aaatggtata gtattacatg catatgtatt
     2521 tttatagtag tagcttttta tatgtttata tttcctaata aaatagaaac tattgatact
     2581 tcatctgcat atagctttgt tgaaaagaaa gtaccaaata gtgctgtgta tcaaggatac
     2641 aaaaagaatc cagttgatgg tactactaca atttattaca gctatgataa ttcaactcat
     2701 atagttagat tatcacatcc agaagactat tcaaggaaga ttaattggga taaagtttca
     2761 aacatcagat ttgattaatc tttaatattt ataaaagtct atatatctaa ggcaaatata
     2821 ttattaatct tattcatagt tgagcacttt ctattataga agtgcctctt ctgatttata
     2881 tcataaaata ttactcaaca atttgtaaaa tatattagtt tataaaatat attgaaaaga
     2941 taatattata taatacgatt gtgtaagata ttgataaaaa atcgtaataa attttcatta
     3001 gtatatgcta atattttttt acaattttcg taattaatta atagaaaaat atattataat
     3061 aatattatga aaatttaaaa aagtaagaaa gaggtaatat tatgagaagt gatataaaaa
     3121 aaggaataga aggagctcca aagagagcgt taatgtatgg aatgggtctt actaaagaag
     3181 aaattgaaag accattaata ggtatagtaa atgcacaaaa tgaggtgatt cctggtcatc
     3241 tgcatcttga tgaaatagca gaagctgcaa aaaatggagt aagaatgagt ggtggtttgc
     3301 cattagaatt tcctgctata ggggtatgtg atggtatagc tatggggcat gtcggaatga
     3361 attattccct ggcttcaaga gaattaatag cagattcaat agaagctatg gctatggcac
     3421 atggttttga tgctttggta cttataccta attgtgacaa gatagtgcca ggtatgttaa
     3481 tggctgctgc aagacttaat ataccaagta ttgttgtgag tggagggcct atgcttccag
     3541 gtaaaaaaaa tggaaaagta tatgatttta actctgcaat ggagggtgtt ggagcatgta
     3601 aagatggaac agtaagtgaa gaagaattag aagagttagc catgaactct tgtcctggat
     3661 gtggttcatg ttctggtctt ttcacagcaa atagcatgaa ttgtttaaca gaagctttag
     3721 gcatgggtat accatataat ggtactgctg cgtcacattc tggagaaaga aaaaggatag
     3781 caaaatatgc aggtatgtat gttatggagt tacttaagaa cgacataaaa cctagagata
     3841 ttttaacaat agatgctttt aaaaatgcta tagctgtgga tatggcaatg gctggttcta
     3901 caaatacagt acttcactta cctgcaatag cttatgaatc aggaatagag cttaacttag
     3961 atttttttga tgaaataagt gaaaaaactc cttgtttaac aaaattaagt ccaagtggaa
     4021 aacatcatat tgaagattta catatggcag gaggaatacc agctataatg aacgaacttt
     4081 caaagataaa tggaataaat ttagattgca aaaccgtaac aggcaagact ataggggaaa
     4141 atataagaaa ttgtgaaata gaaaatgaag aagtaataca tacattaaag aatccatata
     4201 gtaaccaagg tgggcttgca atattgaaag gaaatcttgc tctaaatgga gctgttgtaa
     4261 aaaagtcagc agttgcagaa gaaatgttag ttcatgaagg accagcaaga gtttttaatt
     4321 cagaagaaga agctgtaaat gctatttttg gtaaaaaaat aaacaaaggt gatgttatag
     4381 ttataagata tgaaggtcca aagggtggtc caggaatgaa agaaatgcta tctcctacat
     4441 cagcagttgc aggaatggga cttgataagc atgtagcact tcttactgat ggtcgttttt
     4501 caggggcaac tagaggggca tccataggcc atatttctcc agaagctatg gaaggtggtt
     4561 taatcggact agttgaagaa ggagatataa tttctataaa cataccagac aaaaaattag
     4621 agttaaaagt agatgaagtt gaaatagaaa atagaaaatt aaaatttaaa cctttagaac
     4681 cgaaaataaa gcatggatac ttaagtagat atgctaaact ggtaacatca gcaaatacag
     4741 gagcagtttt aaaatagtag actatattta tgtaaattag aaaaagttaa gaaaatatta
     4801 attggatatc ttaggtgtta tagcactaat gaggtatcct ttttattttg tatgaaaaac
     4861 atagcaaaat cttgattatc tttgaaaaca atacatctct attataagga atacgagtac
     4921 agcatagaga tgtatctttt agaccttgtt gaggtaatac aactttttaa aagtttgatg
     4981 tggaatgctt ttcgttcctt ctgaactatg aagaaattga aagtaagtta attcttgaca
     5041 taataaatat taaaatgtat aataaaaaac aaacagacag tctgttttta ggagggaaat
     5101 atgaatagag aagaaaaaag taaaaatagt aaagaaaaaa ttatccaatc agcattttca
     5161 ctattttcgt ctaaaggata tgattcaaca tctacacaag atattatcaa tttatctggt
     5221 ctatctagag gtgcaatgta tcatcacttt aaaactaaag aagatatact gagaagtgtc
     5281 acaaaagaac tttactcaca aatgaataat tttttagagc atcttgttgc tgatgacacc
     5341 cttacagcaa atgaaaaaat aatagaattg gttgttcata gtgcgaatga ttacacacgt
     5401 agaaaaatgg tacattgtag ctggttagaa aaaatcccat tcgctttaat agaggaagtt
     5461 cgtaatctca acaatgtagt tgcacccaat attgctaaga taattaaaca aggtgttgaa
     5521 aataaagaat tttcttgtga atatccagag gaattagctg aaatgctcgt ttttagtatt
     5581 gatattttac ttgaccctgt attatttaaa cgtgaataca gtgaagtatg taataggttg
     5641 gattttttat tattcatgtt aaaaaaaatg gatattcctc taattgatga gtatggtatt
     5701 caaaaattta aagatttatt taaacaatga taataggaaa agtgaggaat agactatgtt
     5761 ttataatgca aaaagttgtc aaatgatgat aggaagtaat acagtgaatt atattgaatt
     5821 tggtagtggt aagaagacac taattatttt accaggatta ggagatggtt tatttccttt
     5881 acatggaaaa atacaggcga ttgcttttgc ttttagatat aagcaattta caaaagatta
     5941 taaggtttat gtatttagta gaaaaaatca aattacagaa aaatattcca caagggatat
     6001 ggctaaagac caagcagata taatgaaaaa acttggaatt atgaaagcag aagtaatggg
     6061 tgtttctcaa ggtggaatga tagctcaata tctggcaatt gattatcctg agttagttga
     6121 gaaacttgtg ttggcagtta cttcttcaaa gcaaaatgat actatccaaa atgttatttg
     6181 tagttggatt gatatggcga agaagcagaa ttacaatgat ttaatgattg acacagcaaa
     6241 gaaatcctat tctgaaagat atttaaaaaa ataccaatta tttattcctt ttttaggaaa
     6301 agtaggtaaa ccaaaggatt ttaaacgatt tattattcaa gcgacctctt gcatagaaca
     6361 taatgccttt tccgaattaa ataaaataac atgtccaacc ttaattatag gaggtgctaa
     6421 tgataaaatt gttggaaata atgcttcttt tcatttagta gaaaaaatta aaaaaagcga
     6481 aatatttatc tatgagggat tgggacacgc tacatatgaa gaagcacagg attttaatga
     6541 aagagtttta gagtttttaa ataagtaaac acctttgtta aaaatataca tatgaagatt
     6601 ggaaatttaa tgttaaaaat agaaacatga aaatatgctt aactggtatt tttactattc
     6661 ataaccaatt tttgatacat tatctactat aaatacaaat atagcttcaa tgtgattata
     6721 tattgttgta ttggtaaagc acttatacaa acagaggaat tttgtaaatt catattatac
     6781 ccacatttgt taacttatga aaatataatc aaaattttta tgagcttttt atataaaaaa
     6841 tataatgttt gtatacaaaa at
//
LOCUS       DAYATN010000176         6816 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.179, whole genome shotgun sequence.
ACCESSION   DAYATN010000176 DAYATN010000000
VERSION     DAYATN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.179"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(406..1302)
                     /locus_tag="ACGI8A_003403"
     CDS             complement(406..1302)
                     /locus_tag="ACGI8A_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFH0181367.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHKEVSNVILKEDMK"
     gene            1443..2870
                     /locus_tag="ACGI8A_003404"
     CDS             1443..2870
                     /locus_tag="ACGI8A_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0181368.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(3066..5660)
                     /gene="clpB"
                     /locus_tag="ACGI8A_003405"
     CDS             complement(3066..5660)
                     /gene="clpB"
                     /locus_tag="ACGI8A_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFH0181369.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNNFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5697..6293)
                     /locus_tag="ACGI8A_003406"
     CDS             complement(5697..6293)
                     /locus_tag="ACGI8A_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181370.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNLDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6310..6714)
                     /locus_tag="ACGI8A_003407"
     CDS             complement(6310..6714)
                     /locus_tag="ACGI8A_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFH0181371.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         2184 a         1093 c          708 g         2831 t
ORIGIN      
        1 ttttattata aaatttttta taaatatata atttttttat atttatttga ctttctttta
       61 attctttagg atggatttaa attttctata aaaatcatac tattttagct gtttacttgc
      121 ttatttaata gttttatttc tttgattaaa tatctttcag aatctaagcc agatgtctca
      181 aaaaaactga tttataatat aatcattttt tctgctattc ttatttttcc aattttagtt
      241 tcatattaaa atatactttt catagacact acattctttc atactcattt tcacatttaa
      301 ctatacaagt agtcttactc atttatgtac tatactttat taatatttat taagaaaaac
      361 tcaaagattt aaaaatatat attttctata aagtatattt atgaattatt tcatatcttc
      421 ttttaaaata acattactaa cttctttatg ttcatttcta tacttagttg gagatatatt
      481 ataattcctt ttaaataagc gactaaaata atttatgcta tcaaagccac atctcatagc
      541 aatttctgtc atatttaaat ctgtttcctt taaataatat atagctttat caagcctgat
      601 tccatttata aaatcgattg tagtcttacc tgttatttgc ttaaatgttc tacaaaagtg
      661 atgtgcactc aagttcacat gactagcaag ttcttttaat gtaattttat ccatatattt
      721 ttcttctatc aaatcaaaga tacattcaaa tttttctaaa gaatactttc tttttataaa
      781 ttcatcttca gaatatattt tatcaatatg acttcttaat aagagtacta ttaattgata
      841 gatattagct tttatagata actcatatcc aacttcttta ttgtgatatt catcaagtat
      901 agttgtaata cattttaaaa tcagagaatc attttgaatt aaattattaa atacaatatg
      961 atttaaagcc aatggtgcta agtacttggt ttgtaacaaa tcaacttgat tactaaaaag
     1021 gaatgtgggt tcaatacgaa tactatagaa ttttaaatta tctgataagc tttctaaata
     1081 atgtaattca ttactgtttg ctattacaat atttccaggc actaccttaa aattatccaa
     1141 tccacattca agatatgcta taccatcaat aaaataatat atttgtagat gctcatgcca
     1201 atgtctttta aagtttgctc cttttttaat gtcatccttt aaaaaaatat ctataaagtt
     1261 ttcttgacct ttcctgttaa aatatgtatt tttatccttc attattcccc cctcatcaaa
     1321 atagtgctaa ttataagcaa tatagttatt gtattcatta tttcatatcg ctattatata
     1381 aaatgtaact atcattgtaa acattaacat aatgcagtca ttataataag gaggtaataa
     1441 aaatgacaaa ctcttttaaa aaagatgata atagaaaaaa aatcacagtt ttatttgtag
     1501 tcattgccat gacttttatg gcaacattag attctagtat tataaatgtt gctctcccag
     1561 ttcttgcaag caaactaaat gtatctctag catctattga atgggtaata gcaagttact
     1621 caattattat ttgttcaaca ctattatttt ttggaagact tggagatatt attggtaaat
     1681 caagagtatt tcaagtaggt actattctct ttacatcagc ttcattacta tgtggattaa
     1741 gtaattcttt aactttactt atagtttgcc gctttattca aggaataggc gcttctgcct
     1801 acatggcaaa caatcatgga attatcacag aactatttcc aaaagaaagc agaggaaaag
     1861 ccctagggat tttagttact gcagttgcta ttggaaatat ggttggacca tctgtgggag
     1921 gatttatttt gtcaatattt gattggaatg ttatattttt tataaatatt ccaatcggtc
     1981 ttattgtaat ctttttaaat acaaaatttc ttcctaatag taaaaaatct tcagaaaata
     2041 tggataaaac tggtgctata cttcaatttt taggaacaac attatttttt agtgctctaa
     2101 ttagtgctca acaaacagga ttactaaatc catatatatt aattgcacta ttattatcaa
     2161 ttatttttat tatattattt ttgatattag aaaaaaaaca tcctcaaccg cttcttgacc
     2221 ttgaaatttt tagaaatttt aaattttcat tgaatttaat ttgtgctctt acatcattca
     2281 tttgtattgc atcatcaagt attttaattc cattttattt acaaagtact atgaaacttc
     2341 cccctattca agcaggttta tttatgatac tatcaccttt gattttagcc attttttcac
     2401 ctatatttgg gaatatatca gataaaatta agtcagaaaa aattatttta attggtcttc
     2461 tcgttatgag ttttggattc tttctaatgt caagattgaa agaaagttct gcccttattt
     2521 tatttgtaat ttatatatcg ataatatcaa taggacaagc tatatttcaa cctgctaaca
     2581 atgctttgat aatgtcaagc tgttctagaa gtaagctagg agttgtagga agcataaatt
     2641 cccttgtaag aaatttagga caagttatag gtattactat ttctacaaca cttttatata
     2701 attttatgag tattaaagct ggatataggg tcaatgatta tgtgattaat aatgataaaa
     2761 tatttgtatt tggaatgaga aatgtttata ttattgttac acttgtttgc ttaataggtg
     2821 caatattaat tggcttttat ttatttaaat ataataaaaa tgaacaatag tttttataat
     2881 tatattcaaa ttatgattat attataagaa atttactaaa atacttcctt tgagttaaat
     2941 ttaatttgga catattgact taaaaatgtt ttattaattt gataaaatat ttcttttcct
     3001 atagtttata aataaaaatc agctgagcaa aatatttagt atattatgct caactgattc
     3061 ttttattatt taactaaaac ttcaatattt tcatcttttc catctataac tatagtacat
     3121 ccattataca catctccagc aataagtttc tttgcaatta tagtttctaa tgtatttcct
     3181 atatatctct taagaggtct tgctccatat acagggtcat atccttctct caccataaca
     3241 tcttttgctg aatttgtaac ttcaatagta atatcttttt cttttaatct attttttaaa
     3301 tctttcataa atatatctat aattttcttg atattttctt ggtctaaagg cttaaacatt
     3361 ataatatcat ctactctgtt taaaaactca ggtttaaatc tatgcttcat ttcattcatt
     3421 actaaattgt tagtagtctc tgttatattt cctcctgctt ctaatagata attacttcct
     3481 atattagaag tcattattat aagagtattt ttaaagtcta ctgtcttacc tttattatca
     3541 gtaagtctac catcatctaa tatctgtaag aacatgttaa atacatcctc atgtgctttt
     3601 tctatctcat caaatagtat tacagaataa ggagcacgtc taactgcttc tgtaagttga
     3661 ccaccctctt catatcccac gtaccctgga ggaggtccaa ctagtcttga tacagcatgt
     3721 ttttccatgt attcagacat atctattctt attatattat cttcactatc gaataaattt
     3781 ctagcaagag tttttgcaag ttcagtttta ccaacacctg tagggcctaa gaaaataaat
     3841 gaaccaattg gttttctttc atcttttaat cctgctcttg cacgtattac tgcatttgaa
     3901 actgctgtaa ctgcttcatc ttgtccaata actcttttat gaagttcatc ttctagttta
     3961 agtaattttt ctctttctcc ttcaactagt ttagttactg gtatacctgt ccactttgat
     4021 acaatgctag atatttcttc ttcagtaact tcttctttta aaagagaatt ttcagaacca
     4081 tcattcatat tttcttcgta ctgctttatt tgttcttcta acttaggtat ttcaccatat
     4141 ttaacctctg ctgctttgtt gaaatcatat tctctttcat acttttcagc tttaccttta
     4201 gcttcgtcta attgagcctt taagttttta atatctaata tttgactctt ttctttttca
     4261 tattttgctg tcatttcatc atttttagat tttaactctg ctatttcttt ttgaatatca
     4321 tccaatcttg atttactttt atcatcattt tctttaagta atgcttctct ttcagtttct
     4381 aatgtaaata actttcttct aacaacatct aattctgttg gcaatgaatc tatttcacta
     4441 cgaatcatag ctccagcttc atcaattaaa tcaattgctt tatctggtaa aaatctatct
     4501 tgtatatatc tatgagaaag ttttgctgct gctacaatag cattatcatg tattcttact
     4561 ccatgatgta tctcaaatct ttcttttaaa cctctaagta tagatattgt atcttcaaca
     4621 gtaggttctt ctgcaattac aggctgaaaa cgtctttcaa gagccttatc tttttctata
     4681 tactttctat attcatcaaa agtagttgca ccaatacagt tcaattcacc tcttgcaagc
     4741 ataggtttaa taagattacc tgcatccatt gaaccttctg ttttaccagc tccaacaata
     4801 gtatgaatct catctataaa cagtataatt tttccatcag aactttgaac ttcttttaaa
     4861 actgccttta atctttcttc aaattcacct ctatattttg caccagcaat taaagctccc
     4921 atatcaagtg agaatacaat cttatctttt aaaccttctg ggacatctcc tcttactatt
     4981 ctctctgcta acccttctac aattgcagtt ttacctacac ctggctcacc aatcaataca
     5041 ggattatttt ttgttcttct tgataaaatt cttataatcc ttcttatttc ttcatctctc
     5101 ccaattacag ggtctaattt atttttcttg gctaaatcta ctaaatttgt tccatatcta
     5161 gcaagtgcat catatgtgcc ttctgggtct tgtgtttcta ctctttgact acctctcact
     5221 tgtgataaaa tatttaaaaa gttattctta tttatattat attgttttaa tattttacca
     5281 acagctgatt ttgattctgt ttccatcatt gcaagcataa catgctctac acttatatat
     5341 gaatctttaa attctttaga aatactctct gcctttataa gcacctcatt tatttttcta
     5401 gtagctgata ctccttgtga gtcagcacct tctccatata cctttggaag cttatcaatt
     5461 tcaaagttta cagtatttct caaagaatca attgatatat tcattttttc gattatattt
     5521 ggtatcaatc catcctcttg attaataagt gctgagaaaa gatgaattac atctacttgt
     5581 tggttatgat tttttactgc ttcgttataa gcctcattta aacttttttg aactcttaaa
     5641 gtcatttttt ctacgtccat ataaatccct ccattttaat attatatcca aataaattaa
     5701 attctctcat ttataattat tgattttcta agctctaaaa gactatcaag taaattctcg
     5761 tttttttgta tatcgaaact caatgttaaa ttaaatgttc catctaaatt aaaaaaatcg
     5821 aatacttctt ttgattttgc tattaaatac aagtcttcat ctataagtct tttttcacct
     5881 attaataaat actgacattt atctataaaa ctctttttaa aatcaatagt gtatagattt
     5941 tctaaaatat tatattgata aatagcaata ttattaaaca cttttaatat gtttgaattt
     6001 acaaaattgt aaatttgatt attgttatac agtgatattt gttcgtatat atattcccta
     6061 aataaaggat gaatgttttg cttaagtttt aaagtaactt cagctggtat ttcaaaataa
     6121 agcttccagt acttagagtt atttttttct aaaataaaag ctactttgtt caaactggta
     6181 gaactaatct cctttagtgt acaattaaaa ttatagtgtc tcaaactagt tttttcattc
     6241 tcaaatacat agtcaaacca ctgtagaaca tttctatcaa gttttaaact catataaaaa
     6301 ccttcttcct tatttactaa ccttattagg tctttttatc tgattatatt ctgctaataa
     6361 actttcttta tcagctctac tacctttata tgctatatgt ggatttatca tatactcacc
     6421 atttttcact ctttgtaaaa aatccatttt tttcagctcc gtaaacaatg aagataaaga
     6481 agtttttgga tattcaaatt cacaagataa ttcatataat gtttttctaa tcttattatt
     6541 tttatccata gagtttatta attttaatgg aattaaatcc ctcttagtat acattccttc
     6601 tactacttta atataattct taaaacaaac attcatatac ttacttttat tcataattaa
     6661 acttttgctc tctgtatatt cctgatttaa atcctttttt aatgtcttat ccaaattaaa
     6721 tatctcttct cactctcctt atctctaatt gataatataa atatattaca aaaaaatatg
     6781 tatgtcaaaa ctgaaaatgc aaaaagttca ccttat
//
LOCUS       DAYATN010000177         6737 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.180, whole genome shotgun sequence.
ACCESSION   DAYATN010000177 DAYATN010000000
VERSION     DAYATN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.180"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(222..1436)
                     /locus_tag="ACGI8A_003408"
     CDS             complement(222..1436)
                     /locus_tag="ACGI8A_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFH0181372.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            1862..2578
                     /locus_tag="ACGI8A_003409"
     CDS             1862..2578
                     /locus_tag="ACGI8A_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFH0181373.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            2713..3339
                     /locus_tag="ACGI8A_003410"
     CDS             2713..3339
                     /locus_tag="ACGI8A_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0181374.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(3539..5083)
                     /locus_tag="ACGI8A_003411"
     CDS             complement(3539..5083)
                     /locus_tag="ACGI8A_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0181375.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(5109..5750)
                     /locus_tag="ACGI8A_003412"
     CDS             complement(5109..5750)
                     /locus_tag="ACGI8A_003412"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HFH0181376.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            6020..6214
                     /locus_tag="ACGI8A_003413"
     CDS             6020..6214
                     /locus_tag="ACGI8A_003413"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFH0181377.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFD"
BASE COUNT         2491 a          873 c          989 g         2384 t
ORIGIN      
        1 tattgtctaa ctaattataa tctatccaaa atgaaaatgg gtgtgagata ttattacaac
       61 aaattgtatt agtattaaaa agattaaaaa attaaaaaac taaacagtta aataattaaa
      121 cgattaagtg actgtcatat aacaatttat aattttattt aacagccact taggtattta
      181 agagcgatag aattgagtgt atatcaaatt ataataattt cctagtaaat aattgaaaaa
      241 taaggtagtc catttttata tttgatatct ggtttacctt gaataagtgg atatagataa
      301 tttagggctt catcagttat ataattacat tcttcattta tccactcttt aggaaaatat
      361 tttgtcttgt tagctatttt agaaatatca gtatgactaa cttcatactt ataaggatta
      421 ttgcatgttc tattaatgat agccataact cctgtatatc cttttttaga tagttctatc
      481 ccatatgcac caacttcagc tgcttgtttt aaatcagtat ctgaagctaa atgcatagca
      541 catctttgta ttacaccaag ttctaaggtt tttactcttg ttgtaattga tgatgatact
      601 ataagattct ttagatagtt gcttactcca cctaattggg aatgaccaaa tttatcatga
      661 gcagaagttt tttgttcgca tataaagttg ccgtatttat cccttatacc ttctgatgca
      721 actatataag ccttattttt ttctttaaat atttttctaa catcttctat aaacttatct
      781 tcatagaaat caatttctgg aaggtatatt aaatcaacaa gtggagtatc attaagtcta
      841 gctagtgaag aagaagctgc taaccatcca gtatctctac ccatagtttc tactataaat
      901 ataccattat ttttgtatat agaaaaatct aagtatgttt ccattattag tgtagaaata
      961 agctttgcag cagaaccaaa tccaggacag ttgtcagttc ctaataaatc attatctatt
     1021 gttttaggaa tacctataaa ttttatttta caagtagtgt ttttgcagta ttgagaaaat
     1081 ttcatcactg tgtccataga atcatttccg cctatataaa ataatgtatc tattttatat
     1141 tcatctagta tatttataac tctatcataa tcataagaat ttattttata atcagccagt
     1201 ttgtatctac aagaaccaag accagacgaa ggtgtttgct ttaaatttaa tacattatct
     1261 ttgtttaagg agtctgaatc tattatattt ttgtttaata taccttcaat cccattgata
     1321 ccaaaatata ctttatcaaa gtatttagtt tctaaatttt tttcataaat accaactgca
     1381 gaagcattga taacagctgt agggcctcca gattgggcaa taatacagtt tctcatttta
     1441 catctccctt tcattaatat tgttatgaat ggtacttttt atcgagtaaa tttatattta
     1501 tagtctgatt atatattaaa tgaactagct atatcaattg agttagtata gacaataaat
     1561 gccataagaa tgtggtaaaa aactaacttt ttatatacaa ttaatgtcta taatttacca
     1621 caatataaag gtacttttaa ggagtagtca aagtgttgtg aaaaaaaata tatcagtgta
     1681 atattaattt acaaagcaaa ataagttcac agttaacaaa agcagaataa gttcataaaa
     1741 gaaatatata cagcaataaa agtaatgcta aataaaagta atactggatt aaaaaatatt
     1801 aaaaaaatat taaaaactaa tatttaatac taattaaagt aaaatatgga ggtatacaaa
     1861 tatgaaacac ataaaaatag cagcaggatt agcacatgta aattacggtc atatatcagc
     1921 cgtagttaaa gaagcaatgg atgcaggtgt agactacgta cattcagatg cagcagatat
     1981 gcatgactta aaaaatatgc aacttatggg tggtcatcaa ataatagaag caataagacc
     2041 ggtgacagat aaaccaatag aatgccatat atacacaaga gattgtgata gattatttat
     2101 agaaaaaata gcagcagcag gatgtaactt gttgataata ccagcagaac attttttagg
     2161 tgctcctctt gcatacataa taaattactg tagagaattt ggtatgaaag taggattaac
     2221 aattggatgt tacacaccac tatgttttgt tgatgaagct atatatgata tagatagatt
     2281 gcaaatagtc gttcatggtg ttgatgaaac tgatggaaaa gataactggg gatggagaaa
     2341 gagtgctgtt gatttagtaa aaagagctag aaaaatgata gacgaaaaga atcctaagtg
     2401 tgaattagca atagatggtg gactaagagc agacaatatg gagccattaa tagaatgtaa
     2461 tccagacgtt gtaatattat cttcagcaat attcaaggat aaagatggta taacagcagg
     2521 tgttaagaat tgtagaaaag ctatagatga agcagctaca aagtttggtt tagaataaga
     2581 taataaacta tgataaaaga gatgctttct taatgtttta aggaagcatt aattttatga
     2641 aaaactacag gaagctagac taatttaatt gattttttat actgaaacaa tataatcata
     2701 ggaggttatt aaatgaaaaa agaaatgata aaagattatt tactgataac aatatcagca
     2761 tttttaatgg cattagcagt taattttttc tttgcagaac atacattagc tccaggaggg
     2821 ataacaggat tatcagtcgt acttagcaca ttcttatcaa tgcctgttga gaatatatca
     2881 ttaggaatat ctatgccact tttagtaatg ggattattat ttttagggaa aggttttgga
     2941 ataaaaacat tatatataac tcttatgggc ccattattct taaaagtaat accacaaact
     3001 cacataactg acaatctatt agtagcagga gtgataggtg ggttattggt aggaactgct
     3061 atagggatag caataataag gggctgtagt actggtggaa ctgatttagt tgcaatgctt
     3121 attaataagg taattaagtt tctgaagttg ccagttatat tatttatgct agatggattt
     3181 ataatagtag cgtctggaat aataagtaag aattttatga tatctatata ttcattgata
     3241 tctttattac tcataataaa aacaatagga tttgtaacta caaagtttgg taagaatact
     3301 gaagaaatta ataatataaa taatgcttgt gctcaataaa aataataaaa tttactttaa
     3361 atataaaagt ttaaaaaata taaaagctta aataaaaaga ttaaatataa aaaatgaagg
     3421 tgtctcaaaa tgaatttttt agttcatgag gcactttttc tatgaaatca aatatatttt
     3481 tatcatttgg acaatgaaaa ataggtttat ctctatgttg agacaagcct atttttgttt
     3541 atttataaga taatatttta tttttttcag aatatagttt ttttataatt gtactaagtc
     3601 tagaaatatc attcttactt aaaagagcat ttatatttat cagttcagca ttatgttcaa
     3661 agtctacagg agcagtggtt agtattaagt ccacatcatc aaagttcgta aatgaaatag
     3721 aagatagagg tatagattta actatattaa tctgattaaa ttcaaaactt aacttttgga
     3781 ctaataaatt agatacacca tcactacagt gacatattaa aatagtattt aggggagatt
     3841 tgcttctatc tattgctgat attaaatata aagcaagata tcctatttcg tcatcattga
     3901 cttcataatt catcaactgt ttgataacag tattagactc tttagcagcc ttataagtgt
     3961 aaggaaatgt tctctttata tcatttaata tgggatttgt aatagtaaac ccatagttta
     4021 ttctgtgtag aactgattta aggtgattag caagagactt tattaatatt aaatcatcct
     4081 ttaagtttaa atttagtata cttccccaat gaacagctat tgcttcagat agattatact
     4141 ctgtaatttc ttcattttta agaggttcaa gagtagtagt tttctttatt gatttaagta
     4201 atccttctat atgaagaagg atataacaga cctcttcatc aataaattct acgtcaaaag
     4261 cttcacttag gttagcacat aaaatttttg atttttcaaa gtattcatgt tctaataatt
     4321 ctctaaaaaa accttcagat aagttaatat atcttttaat tgaaattctt ttaatacata
     4381 tagctatatg tattaagaag tttatataat actcatcact aaggttactt ttttctactt
     4441 gtaaggtgtg tagagtgatt tgagataatt tctcaaaatc atagttaaat agctccttaa
     4501 atatttttgt tgatggggta tctttgcatt cattagattt cgagaataat atttctttaa
     4561 gttcagtata acttttatta accgtagcaa gttcaaaaat tgcttttcga atatctttct
     4621 cttttccctc taaatatact ccattattgg tttttctaac tagagtcact ttaaagtttt
     4681 gcaaaaaatt ttgaataact accaagtctt tatgcacagt agctctacta acatgtagtt
     4741 cattgcaaat atcacgaatc ctaaacttag aatttttaat aaataattta cttagaatat
     4801 acctttttct atcttcagga gagtatgcat atatacaatt tgatttatca ttgatatctc
     4861 taataacttt aagttttata tcttcattac ctagaataat aacccctgaa ccagtttttt
     4921 tatctaggga taaattaaac tctaaaagcc attcatctaa aataacaagg tcattacgaa
     4981 tagttttatt actaactttt aattcttcgg caatagtatc tacagtaata tagtctgagc
     5041 tgttgagtaa taattcagca atatctaata atcttttgtt caatgcaaac cctcctaaat
     5101 cttagttact atttattaac cttggaccaa tctctttcaa aagtattggc accatctatt
     5161 gtaagctggt taaaagaagc ttttttaaat aaatcatatg ttacagctgc ataatcacat
     5221 ccacaaagag atgcttgagt taagtggaat acatttttaa tagaagctgc aacaacttta
     5281 gtattatatc catttattga tataaggtta catatttctg taaggttttt tataccatca
     5341 ttcccatttt catcatttct acctataaaa gggaataggt aatcagcacc tgcatttata
     5401 gctaatgatg cttgatttac attgaaaatc aaggtacatg cagtttttac acctttacta
     5461 tttaatatgt ttataagttc taagccaact tcagagaaat ttatttttat tatagtgttt
     5521 ttatttatat ttaatatctt atcacatgaa ttaattaatt cgtataaatt atttccaatg
     5581 acttcagcag ttaatatttc tggatttaaa ttagcacatg tctcaataaa ttcatggaag
     5641 tttactttct ctttaagata catagatgga ttagcggtta ttcccttaaa tccatacttt
     5701 agacactttt gaatttcatc aagattagca ctgtctatca aatatttcat aatttaattc
     5761 tcctttaagc tatttgtcat aaaaaagtgt atatactcta tttttattat taagtcaatt
     5821 atacaataag attgattata aaattataac atgggtactt aataaaattt acataatgat
     5881 atggtaataa taaaacattg attgtctaat aattaccata agctttagta aataagagtt
     5941 aacagtgaag gtattgcctt aaaaatccta tgatatgtat aatttgaata tgaaggcaat
     6001 aatttaaggg aggaaaaata tgattaactt ttatcaacca tcaagaataa attttggaca
     6061 aggaacttta agtgaattac ctagaataat aggaaaatat ggaaagaaat gtttgttagt
     6121 tactactcca aatgtagagc cattagacaa gttatataaa agagttacat tgaatttaat
     6181 agaaaaaggt atagaagttg tacactttga ttaaaaaata gctacgcaca ctcgtgactt
     6241 tagtcatgag ttaatagcta aaatagtcag catataggga aacctatatg tagagatagg
     6301 atagaaccta tccaatacta cttgaattgc tggaaacccc taaagctaac caaactaaaa
     6361 cataaggatg aaataaacct aagtgtgaaa gttacgaaag tagaaaaaat tggttagatg
     6421 gtataaggtt aaatcctaag tactttaaaa tgggcaatca gcaggtaagc tccgaatagg
     6481 agaaacttca acgactattc ctcttgaggg aagtacacta caagttatcg gtagtggaag
     6541 tgggtagacc ttaacggata atgccgaagg ataagatata gtctgtgctt gcatgaaagt
     6601 gtaagaagtt cataagagaa ctgcataggt agtagcgaac ttatgtgaac gacaacctca
     6661 aaaatgataa aagttaagac ttacggttct taaatatgtt atttaaaatt aaatataaaa
     6721 ccttctaaat aattgtt
//
LOCUS       DAYATN010000178         6690 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.182, whole genome shotgun sequence.
ACCESSION   DAYATN010000178 DAYATN010000000
VERSION     DAYATN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.182"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(825..1385)
                     /locus_tag="ACGI8A_003414"
     CDS             complement(825..1385)
                     /locus_tag="ACGI8A_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HFH0181378.1"
                     /translation="MLKKLKKMLLITCCLFMMSSTAFAETQNSEGKSFTISGYTFMYE
                     NAPQEIKDKYKENCLDIGVEPQPNDEIFITPSELKTYSNIDMYSARSSSFTVTYHYSY
                     YKVTGDKNYTVNTSTIVKYGNSGNPVHLVQLLLKKVGYALEVDSQFGNTTLSKVKSFQ
                     GSYGLTVDGIVGSATWKKFASATNLD"
     gene            complement(1431..2414)
                     /locus_tag="ACGI8A_003415"
     CDS             complement(1431..2414)
                     /locus_tag="ACGI8A_003415"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFH0181379.1"
                     /translation="MSNLILTDILRTSITSSLVICFLLLLRTTIFRIFNKGFNYYIWL
                     IVIVRLLFPFSYFTYTISDSKFQYKPILNEVDYSNTVLIIYLTIVILLAIYRFIKYLK
                     FKSLVIDLSYDIENLEINNLYNNLLKELNIEKHIKLKYTYEINTPSFFGLFDNYILLP
                     PHNYNLNELHWILKHELIHYKSKDLYIRYLVLFLKCVYFFNPFIYLLDRIIYHSCELH
                     CDEVVLKGCSLENIKSYANTILDSIERSSRDSNNYTTGLHRKSNFEKRVYDMFKNKGK
                     SGILIALILCLLSSFTYFKFDSIISKPINHSLDAPLPVNTGKEKSFTITID"
     gene            complement(2427..2789)
                     /locus_tag="ACGI8A_003416"
     CDS             complement(2427..2789)
                     /locus_tag="ACGI8A_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0181380.1"
                     /translation="MIEKIPRAELKVMMLFWDTDKVLTSRDIVNIMSEEWKQTTTLTL
                     LSRLSKRGFLEPTKKPTHTTYKILIDKKSYLEFATSDFVKNVHNDSIVSLVDSVNKEK
                     IGKDELASLRAFIKKLKK"
     gene            4032..4679
                     /locus_tag="ACGI8A_003417"
     CDS             4032..4679
                     /locus_tag="ACGI8A_003417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181381.1"
                     /translation="MNKKLEKPFVYKREYDLTGYDVEILQKYELEQAIYVYVGSSCAY
                     NMRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNIINDKLYYSNIAEF
                     EEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNITKNGNTNYLKVK
                     NKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSRPSRVFLK"
     gene            4954..6591
                     /gene="repA"
                     /locus_tag="ACGI8A_003418"
     CDS             4954..6591
                     /gene="repA"
                     /locus_tag="ACGI8A_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFH0181382.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNS
                     KQLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINR
                     YALGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVS
                     EVTNTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKK
                     YCKDVKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQ
                     VISVAREWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIY
                     SDEDTLFNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITY
                     KRKKKVKNIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYT
                     ISYFVTMAVIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQ
                     QRISNMIESLLDTKILDVWETKINDRGFMYYTYRLNK"
BASE COUNT         2733 a          699 c          938 g         2320 t
ORIGIN      
        1 aaaaaaaaaa tggcatcaaa gtattgaaat taagccgttt taaaaatttc ttttggtaaa
       61 ataattgtac atatatatgt agtatatata tatatgtttt ttagagaatg tataactaga
      121 atatagagct agaatataga gaatgtataa ctagaatata gagctagaat atagagaatg
      181 tataactaga atatagagct agaatcctaa ttagtaggtg cttttttaaa acaagttaaa
      241 aatcaaaaat agtattagta agcattggaa atgctagatt ctaaaataga aaagtaaaaa
      301 attggtgcac tatctaaact tatctatatc gctttttccg tcgtttggtt ctctagttac
      361 gatacagggg atatgcttat attgagttat agtactaatc agtgcttaat atagttaata
      421 aaattatagt taccatagtt tagtaactat gatgtatgtt agttagaaac ttgcatttca
      481 tgagttgtaa tatcttaaag tcagtagttt tgattttaaa atattaattg cactgttaca
      541 aatgacttta ttaataaaaa attaggcagc ttaaaaaaag tttagaataa atttgctagg
      601 ctttgctaag ctattctttt aaaaataact ttacaaaaca aacaaataag gacttataaa
      661 tatagataaa aaaagaaaat tgaaaaggat tatagagact tacaaggctt taaatatata
      721 ttcttaattg taaaattatg taattaaaat atttacttta ttataaaaag acatgataaa
      781 cttattatca tgtcttttgt taaacttatt tatatgtaaa aattctaatc aaggtttgta
      841 gcagatgcaa attttttcca tgttgcacta ccaacaattc catctaccgt taagccataa
      901 ctgccttgaa aacttttaac tttgctaagt gtagtgttcc caaattgact gtctacttct
      961 aaagcatatc caactttttt taatagcaat tgtactaaat gaacaggatt tcctgaattt
     1021 ccgtatttta caatcgtaga agtgttgaca gtatagtttt tatctccagt aactttgtag
     1081 tatgaataat gataagttac tgtaaatgaa cttgacctag ctgaatacat gtctatgttg
     1141 ctataagttt ttaattcact aggggtaata aaaatttcat cattaggttg tggctctaca
     1201 cctatatcca aacaattttc cttatattta tcttttattt cttgtggtgc attttcatac
     1261 ataaatgtgt aacccgaaat agtaaagctt tttccttcac tattttgtgt ttctgcaaaa
     1321 gctgtgctac tcatcataaa taaacaacaa gtaattagca acattttttt taattttttt
     1381 aacataattc ctccctataa ttttgaatat atttgaatac cagcaaatat ttaatctatt
     1441 gttatagtaa aagatttttc tttgcctgta ttaactggca atggtgcatc taaggagtga
     1501 ttaattggct ttgatataat tgaatcaaat ttaaaatatg taaatgatga caataagcat
     1561 aaaatcaatg ctattaaaat accactctta cccttatttt taaacatgtc ataaactctt
     1621 ttttcaaaat ttgattttct gtgtaatcca gtagtgtagt tatttgaatc tcttgaactt
     1681 ctttcaattg aatctaaaat tgtgttagca taacttttta tattttcaag ggaacaacct
     1741 tttaatacaa cttcatcaca atgtaattca cagctatgat atataattct atcaagtaga
     1801 taaataaaag ggttaaagaa ataaacacac tttaaaaata aaaccaaata tctaatgtat
     1861 aaatctttac ttttgtaatg aattaattca tgttttagaa tccaatgcag ttcatttaaa
     1921 ttataattgt gtggtggcaa aagtatataa ttatcaaata atccaaaaaa tgagggcgta
     1981 ttgatttcat aagtatattt tagtttaatg tgcttttcta tatttagttc ttttaataaa
     2041 ttattataaa gattgtttat ttcaagattt tcaatgtcat atgataagtc aataaccaaa
     2101 ctcttaaact ttaaatattt tataaaccta taaattgcaa gtaaaataac aattgttaga
     2161 tatattatta atactgtatt tgaataatca acttcattca aaataggttt gtattgaaat
     2221 ttagaatcag aaatagtata agtaaaataa gagaatggaa aaagtaatct aactattaca
     2281 attagccaaa tataataatt aaaaccctta ttgaaaattc tgaatatagt tgtccttaaa
     2341 agtagcagaa aacaaataac tagactactt gtgatactgg ttcttaaaat atctgttaga
     2401 attaaattag acatatatag tacctattat ttcttaagtt tttttataaa agctctcaaa
     2461 gatgccaatt catcttttcc tatcttttct ttattaactg aatcaactaa acttacaata
     2521 gaatcattat gtacattctt tacaaaatca gatgttgcaa attctaagta gctcttttta
     2581 tcaatcaaaa ttttataagt agtgtgtgta ggtttttttg taggctctaa aaaccctctt
     2641 ttagaaagcc tagataatag tgtaagtgtt gtcgtttgtt tccattcttc actcattata
     2701 tttacaatat ctcttgatgt caataccttg tcagtatccc aaaatagcat cattacttta
     2761 agttcagcac gtggtatttt ttctatcata atatgttcct ccatcctaat gttaaaaaaa
     2821 ttaataaatg taattttatt ctattgaaat agagtaggac agtcaattgt tttttgatta
     2881 atgatttaca tatgtagagt aaattaaaag aagtatatca cttgaaaagt tttacatacg
     2941 tagagcattt ttatcaaatc tcttaatttt tttaattgaa ttatatatgt gaatataaga
     3001 gtaaatcaac ttaaaacatg caaaaaatta atatttactg acatgcaact gacaaatcaa
     3061 caaaaatagg aattgataaa ataagtgtat atattgaaat tgctataata catacaaaaa
     3121 tggcataaaa tactaatttt tatgtccttt tctactgaca tgtaaaaaag tcttgtaagt
     3181 attggaatta ctagattaca taggttttag atgcaaaatt gtcgttttgt aaaaaaggct
     3241 actaaaattt ataaaaacaa ttattaattg atataaatta tcacaaaaaa agacatctat
     3301 ttctatgaaa aaaaaatttt cgtctgcatg taaaaatagc tatagtaatg cgtacgaatg
     3361 ttctattatt acagaattat ttgtatgtaa attatattga aaatatctat cagaattaga
     3421 attttatatg gataaaacag aactatcaaa taggagtata tagtaaagat ttatataata
     3481 tgatgacctc gtacaagcct gtaagagccg tttaaaagtt gctttagtat taattatctt
     3541 tgaaaattta aaataggttt aaatggcttg ttaaatatga tgtattacta ttatgtatag
     3601 gggggtagtt atgtagttac atgttgtttt aaaaaataaa tttaactata gttctttcac
     3661 ttccacatta caaaaaataa attgagtgtg tatgttttta agtaaaaatg atgtttaaat
     3721 attgaaaaat agagatatag tgtatgtaaa atttagaata gtgtatgtaa ttttaggcat
     3781 aaaaatatgt gattaaacct taaaaaccag gaatatagtg tatgcatttt taaaaataaa
     3841 aattgtaacc tttttgtaat cttttattaa aatacttact ttaagtctaa aaattagggg
     3901 agatgtaagg atttgggaaa aatagaagat gttataatca taaatatggt attcgtaggc
     3961 ttaaagtcaa aaaggaggtg aaatataaat agatttttag ctaaattaag taagaaatag
     4021 gaggagattt attgaacaaa aaattagaaa aaccatttgt atataagaga gagtacgatt
     4081 tgactggata tgatgttgaa attttacaaa aatatgagtt agaacaagca atatatgttt
     4141 atgttgggag tagttgtgca tataacatga gagctagaag tagtaaatgg agataccata
     4201 taagaacaaa taataagtct atatgttgta acattaaaaa ttttatacat aacttggaat
     4261 tgttttataa aatggaatta aagttgtcag ataatattat taatgataag ctatactata
     4321 gcaatatagc agagtttgaa gaatttgaaa cactagaaaa agctagagag gtagaaagta
     4381 ctattataag tcaatatcaa tttttagatt ctataaatca tatgttaaaa caaaaaataa
     4441 ttttattgag taataaggat agtgtgttaa acataactaa aaatggaaat acaaattatt
     4501 tgaaagtaaa aaataaatac atagaaaaac ataagaacaa gccaataatg agataccata
     4561 tcaactgtca attcaataca gatggaagtg tcaaaagtat tacacaggag tttgaaccaa
     4621 tattggaatt aaacaaaaaa aataccctaa gccgaccaag cagagtattt ttaaaataat
     4681 attttaagat aacaacaaaa tgagataata ctactagaca atgacaactc aactaccaat
     4741 tgagtttatg gagctaccaa ctccaatatc ggtctaactg attaagtatc tgtagttata
     4801 taataatatt gctatcaatt ttagcatctt aacaatatta ttatacatac taagctaaaa
     4861 ttattcaata gttgtaaaag ttgattagtc aataagtata tatttaatgt agtgttatct
     4921 cttaaaaaaa ctagataagg agataataaa tatatggaac aattagattc aaaatataag
     4981 ttgaaaaaat ttctaatggc agtatttaga gatggtatag gacaaggaaa taatcttatt
     5041 gataatgaat atgttagagt atttcaaaat aataaaagta atagtaaaca attagaactc
     5101 ggagaagaat ttaaagaata tagtaaaaca acttttttta aaaatataga tgatatagta
     5161 gaatttacct tcgcaaaaaa tatttattat gaaaatacat tttttaacct atgtacaact
     5221 gatggaaaag caggaaccaa tgaaaactta ataaatagat atgcattagg atttgatttt
     5281 gacaaaaaag aattaggaca aggttttaat tataaagata taattaattt atttactaag
     5341 ataggattac attatcatat cctagttgat agtggaaatg gattccatgt ttatgtgcta
     5401 attaataaaa ctaataacat taagttagta tcagaagtta caaatacatt aataaataaa
     5461 ttgggtgcag ataaacaagc aaatttatct actcaagtat taagagtacc ttatacatat
     5521 aatattaaaa atactactaa acaagtaaaa ataatacacc aagacaaaaa tatatataga
     5581 tatgacatag aaaagttagc taaaaaatat tgcaaagatg taaaaacagt aggtaatact
     5641 aatacaaaat atatattaga tagtaagcta ccaaattgta tagtagatat tttaaaaaat
     5701 ggtagtaaag atggacataa aaacctagat ttgcaaaaaa tagttgtgac tttaagattg
     5761 aggaataaaa gtttaagtca agtaatatcc gttgctagag aatggaacta tatatcacaa
     5821 aatagtcttt caaatagtga gctagaatat caagtcaagt atatgtatga gaagcttaaa
     5881 acggttaatt ttggttgtac aggttgtgag tttaatagtg attgttggaa taaaatagaa
     5941 tcagatttta tatatagtga tgaagatact ttgttcaata tgccacataa gcactcaaag
     6001 gatttgaaat ataagaatag gaaaggggtt aaaataatga ctggtaatca attgtttatc
     6061 tataatgtgt tacttaacaa taaagataga gaattaaaca tagacgatat aatggagctg
     6121 ataacctata aacgtaagaa gaaagttaaa aacattgtta tgagtgaaaa gacattaaga
     6181 gaaacattaa aagaacttca acataatgat tatattacaa aaacaaaagg tgttacaaag
     6241 ctaggaataa aagatacata caatgtaaaa gaagttagat gtaatataga taaacaatat
     6301 actattagtt actttgttac catggcagta atttggggaa taatttcaac tgaagaatta
     6361 agattatata ctcatatgag atataagcaa gatttattgg tcaaagatga taaaataaaa
     6421 ggaaatatat taagaattaa tcaagaggaa ttagcaaaag atttaggagt aacacagcaa
     6481 agaatttcaa atatgataga atctttatta gatactaaaa ttttagatgt atgggaaact
     6541 aaaataaatg atagaggatt tatgtactat acatatagat taaacaagta gatttttgat
     6601 aggattagaa ttgattttct agtcctattt ttatgcaaaa aaactaatta ataaaaattt
     6661 cttttggtaa aataattgta cgagaattgc
//
LOCUS       DAYATN010000179         6621 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.183, whole genome shotgun sequence.
ACCESSION   DAYATN010000179 DAYATN010000000
VERSION     DAYATN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.183"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            401..1675
                     /locus_tag="ACGI8A_003419"
     CDS             401..1675
                     /locus_tag="ACGI8A_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFH0181383.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1882..2208)
                     /gene="trxA"
                     /locus_tag="ACGI8A_003420"
     CDS             complement(1882..2208)
                     /gene="trxA"
                     /locus_tag="ACGI8A_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFH0181384.1"
                     /translation="MYIMVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYE
                     ALGNEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKN
                     KIESYM"
     gene            2466..3218
                     /locus_tag="ACGI8A_003421"
     CDS             2466..3218
                     /locus_tag="ACGI8A_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFH0181385.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3205..4086
                     /locus_tag="ACGI8A_003422"
     CDS             3205..4086
                     /locus_tag="ACGI8A_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFH0181386.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4312..5667)
                     /locus_tag="ACGI8A_003423"
     CDS             complement(4312..5667)
                     /locus_tag="ACGI8A_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFH0181387.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2545 a          950 c          828 g         2298 t
ORIGIN      
        1 aaaatctttt tttctaatta tatacaatat attttccatt atgttctaat caatttcttt
       61 actattttta caactttatt agtcatattc ataattattt ctaattagtg tttaacattt
      121 tatgtaaaat ttataaaaaa atgtatataa attaaaataa gtgactatac taaaaatatg
      181 atacggataa acaataagtt caacctatct cccccaaaat caagtgagct acagtttccc
      241 ccaactgtag ctcattttcg tgtaatttaa taaacatctc aacaagaatt aattaacaaa
      301 accccttata aaaacataac atatattaaa caagctcttg attaaaactt gagctttttt
      361 aataaaaaac agaatagttt attagataag gggttttttt atgcaattca tggatgtatc
      421 taaatacatt gaaggtgcaa accttaaagt taaaactaaa agtggtgaaa ctgttaaaag
      481 aatatatttt aacaactctg ccactccatt agttttaaaa aacgtagtgg ataacttaaa
      541 ctgtgagatt cctttgctta cttatattaa tgctccagga ataatctcag aaaaaaatac
      601 tttaaaatat gaaaatgtac gctctactat cctcaaactt attgatggag acgaagaaaa
      661 ggattctgtt atttatgtca aaaatgctac agaaggaata aacttcttag caaaattttt
      721 taaagatgaa aatccagata aatttgttat taccacagca atggagcata tggctaatta
      781 cctgcctttt aaagttaatt ttcctacaaa agttgttgga attacagaaa aaggagaact
      841 tgatttatgt caattagaga atatacttaa aaattatgct ggtaatgtat ctctcgttac
      901 agttacagga gctagtaatg tcacaggtat aaccactcct atatatgaaa tagcaagaat
      961 agctcataag tatggtgcaa agatattggt ggatattgtg caagtaattc agcataaacc
     1021 tttttcaatg atgccacatg aatgtgatga gcatatagat tttgttgttt ttagtgctca
     1081 taaatgttat tctccactaa atggaggagc tctggtagga cctaaaaaat tctttgaaaa
     1141 atttaatcct gtactatatg gctcaggttc taccaaattt gtaaatgata acaaaatact
     1201 atatgcaaac gtaccccaac gttttgaagc aggataccca gagtattttg gaactcttgc
     1261 aatgggaaaa gcactgaaca ctttaaataa aattggtcta aacagaattt caaaatacga
     1321 aagagaatta tttttatatg ctaaagaaga aatgagtaaa atacctaata taataatata
     1381 tggagataaa tcaaataatg taattatccc aaatatatct tttaatataa gtaatatgta
     1441 ttacaaagat gtagcaaaat atttagttga taactttgga atagaaactg gagctggagt
     1501 agtaggtgca gatatttatg tacaaaaatt attaggtata agtccaaaag aagcatatgt
     1561 cagatttatg agggaaaatc ctgtaggtct tgttaggata agcttaggaa tgtacaatac
     1621 ttttgatgag gtggatagct ttatacatgc tattaaaact ttggctttta aataagtttt
     1681 ttctattctt ttttatcaat aaatagagag tagttttgtt ttcacaaaaa agcaaattat
     1741 atcaacagtc aatttcatta gcaattagac actcatttgt aactatacaa actattttac
     1801 actatacaaa caaaaaactc attacaatga gtttttttgt ttatatatga gatttatttt
     1861 aattacttca atttacttta attacatata tgattcaatt ttgtttttta aattctcctt
     1921 aggcataaaa cctattaatc tatctacagg ttttccatcc ttaaatatta gcatagttgg
     1981 tactgtagat acttcaaatt tctgagctaa ttctatactt tggtctatgt ccaccttcaa
     2041 gaagtttgct ttttcaacca tctcatttcc taaagcttca tatattggtg atagcatctt
     2101 acaaggtcca caccaagtcg caaagaaatc tacaactact aaaccatctt tattttctac
     2161 ttcatttata aattcattat tatttactat ttttaccata atatacatct cctttattat
     2221 cttcttgata ttaatatact acgacctata taacagttca attaggcact tttttgtaac
     2281 cgcctatttt taatagattt aatattatga taaatagatt tataagatta aatatttata
     2341 agactaaata tttctaatat attttatttt tttcttaata tagttgacat tttttccctt
     2401 aaattataaa atcaaattaa taactacata ggtagttact aatcttttat caaaggagtt
     2461 tctatatgga taatattatt aatgaattac aaaatatagg atttactaaa tacgaatctc
     2521 aaatatacat atcattatta aaggaaagcc ctttaacagg atatgaaata agtaaattat
     2581 caggtgtacc acaatcaaaa gtctatgaaa atataacaaa gctattaaac aataatataa
     2641 ttatcaatgt tggaactgac cctattaagt acataccaat aaaccctaat gaattattaa
     2701 aaaataagaa aaaagaattt gacaattcta tggataatct aaagaatagc ttacagatat
     2761 taaataaaag taaaagtgta gaatacgtat taaacataaa aggagataaa aatatattaa
     2821 aaaaagcaat agaaatgata aaaaatgcaa aacaagaaat tataatttct tgcccttatg
     2881 aagaaattct tgaattaaaa aaagaactcc taattgctta caaaaaaaat attaatattg
     2941 aaatactact tttagaaaac aaaagtctga aaggtcttga taatattcat attcatggag
     3001 gtaataaaac tacacttgaa aataaaatgc tttacaaagg aattattttg attgtagata
     3061 atgatgaaat attgacagga aacttatcag aaggcagtga ggccatttca atttggacaa
     3121 aaaactctaa cattttatat attggagttc aatacataaa acatgaaatt tatatttcag
     3181 aacaaataaa aggaggttat aaaaatgaac aaaattaaga ttaaacaagt tgatgctttt
     3241 acaactatac cttttggtgg aaatcctgct ggtgtcgtta ctgatacctc taacatttct
     3301 gatgaaatta agcagaatgt ggctaaagaa atgaatttat cagaaactgc atttgtttca
     3361 gagtcaaata ttgcagattt taaagtacaa tttttcacac caggtttaga agttgattta
     3421 tgtggtcatg caacaatagg tactttccat gcacttttgg aagaagggaa gcttaatact
     3481 aataaaagta tattttatca agaaacaaaa gctggtgtat tatctgtaga gcttaagaaa
     3541 ataaataatg aaaatatatt tatgatggaa cagaatacac ctgaatttga aaatttagat
     3601 aattgcagaa aagaacttgc acaaatgata ggtgtgcatg aagatagact tcttgaatat
     3661 cctattataa aggtaaatac tggactttgg tggcttgtat ttggtctaaa atctttagat
     3721 gatttaaaaa atatctctcc agatttaaat aaaatcaaaa aatttagtgg tgataataat
     3781 ataattggta taactccatt ttgtatagaa actatagata aaaagtgtga ctaccattta
     3841 agatctatag caccatatgt tggagttaca gaagaccctg tatgtggaac tgggaacgct
     3901 tgcgtatcat cttatatagt acatcacaat attttagata aaactagttt tattggtgag
     3961 caaggtaact ttataaacag acctggaaag gtatatgtaa atataactag agaaaacaat
     4021 aacataactt ctgtaaaaat tggaggaagt gcttatactg tcttaagtgg tgaaattatg
     4081 tattaatccc atacctaata aaaacaaata aaaatactag ctaactaaag ctagtatttt
     4141 tatttgttat ttttatttaa taatcatact ctaacaaata ttataattat atatatcata
     4201 atattgtatt tagaagtaaa attttatttt tattattaat tagttataaa tcaatctaat
     4261 acaacataat tacagtagat taacctataa aacataataa ttgcttactg attatttatc
     4321 gtcctcaaca agtctactta atgtcttatc agataagaat aataatatcg ctgcaacaaa
     4381 tagtattact ggaattatag tatatacttt aatcatatca aatttaagag taaatgcata
     4441 tatatatcca tatcctttac cagctataaa agtagcaaat gtccatactc ccataagaac
     4501 acctagaagc ttcttaggtg catacttact tacaaaagag ttaccaagtg gtgagaataa
     4561 catttcacca agacttaaaa gaataccaaa catgattatc catagtatac tagctttagc
     4621 agtttcagga gctccaactc ctcttgaaaa ttctgcacca acaagcatta agaatgcaag
     4681 acctaagaat attaatccaa gacctgtctt tttaaataaa ctcatatctc cttgtggtct
     4741 actagcaagt ttaaaccata atgctccaag aactggtcct aatactatac atacaagtga
     4801 atttaatgag tcaaaccaag caagaggaac atcaaatcct ccaacattca tgtttacaaa
     4861 tgctgcacca taatcataaa ctgcaagata agttagatac cagaataccc agaaaattac
     4921 agagaagaat gatataagta taatagccca aattctcttc ttatccatgc ttgtaagagg
     4981 agacttatct ttttcttcaa ttttttcagc aactacacct tctttaaaag gtctctttcc
     5041 tgcattccca aggaatctcc atccatatat gaaccatatt gcacctatta cacaaagtac
     5101 agctgctata aagaaacatt gagaaaatcc aagtacacca ttttttgcaa atgttttaag
     5161 gtaaagaata ccaactgcta tagtaccaat aaaagaacct atattaacaa aagaatattg
     5221 tactgaaaat gctgtatcaa gttcttcttg actatcaaaa agctgtccat ttaccgcaga
     5281 tacgttacct ttaaagaatg cagtaccaat tgaaactaag attatcattg catttaccat
     5341 agaaactgta gttgcaatag aaccaagata gtatcctacc cccatgataa gcatacctat
     5401 aggtatagta tatctagctc ctatccatct atctgatata taccccccaa ttataggtgc
     5461 aaggtatgtg aatgcaacaa ggttggcttg cattatagca gcttgtcctt tatctattcc
     5521 aagaccacca tgaactatag ctgttgtaag gaatatatat attaagtact ttgaagcata
     5581 atatgctgct ctttcaaatg agaaagttgt tgagcagata taaaatccca taggaaattt
     5641 acccttcttt tttactgttg agctcataat tttaaatcct ttcttaattg tatttttatt
     5701 ttttacgttt tgtccaaatt ctttctttta tgaaagatat aatattatta ttttgtaaaa
     5761 caatttgtaa ttattattaa gataattgcg catttatttg tagagtacat attctcatgg
     5821 aataaaatta atacacttct taatcataaa tttaaattat ataaattcac tattttcaga
     5881 tatagttata aattaatata ttctcatcta ataccatgaa aaaaccttcg ccatatgcaa
     5941 ttcttatttt ttacgtgata gtattttaca aactaaaatc ccccttttgt agcaacttat
     6001 gtaattacat tttagtgctt ttttaaagga gtctcgaata aagcaacata tattttgttc
     6061 aaataatgtt tttatttatt ttttaaattt atatattact tcacccaacc aattctcaac
     6121 ttaaataact aaactactat ctattcacag attaggctta tttcttacac attagtttat
     6181 aataaacact gtgaatctaa acataaaaac tgttatttta aagtaattac ccatatattc
     6241 gctttgatac tatttattga ggctatactc tttatgtata atatattatt tataaaatac
     6301 ctcaaaatat attattcata aaatacctca aactgaaaac tttttcacga atatttattt
     6361 ttcacctccc cttttttatt tcaccatgta aatcgtacca taaatatgca cttatatgca
     6421 agaaaaaaat ctattaatcg acagtaaata attagaaaaa ttcaaattat atttatcaat
     6481 tatattatat aaaatatttc ctatgaaaat ataaattaaa ttaatgttca gaatagtttt
     6541 tagatatgaa atcttgattt ttttattact ttacttattt gctcaaatat atttttgtat
     6601 gcagtatata ataatcaaat t
//
LOCUS       DAYATN010000180         6589 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.184, whole genome shotgun sequence.
ACCESSION   DAYATN010000180 DAYATN010000000
VERSION     DAYATN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.184"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     misc_binding    109..320
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            483..1772
                     /locus_tag="ACGI8A_003424"
     CDS             483..1772
                     /locus_tag="ACGI8A_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFH0181388.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1826..2905
                     /locus_tag="ACGI8A_003425"
     CDS             1826..2905
                     /locus_tag="ACGI8A_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HFH0181389.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2926..4470
                     /locus_tag="ACGI8A_003426"
     CDS             2926..4470
                     /locus_tag="ACGI8A_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HFH0181390.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4633..5175)
                     /locus_tag="ACGI8A_003427"
     CDS             complement(4633..5175)
                     /locus_tag="ACGI8A_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HFH0181391.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5323..5607)
                     /locus_tag="ACGI8A_003428"
     CDS             complement(5323..5607)
                     /locus_tag="ACGI8A_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFH0181392.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5670..6434)
                     /locus_tag="ACGI8A_003429"
     CDS             complement(5670..6434)
                     /locus_tag="ACGI8A_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFH0181393.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2297 a          814 c         1135 g         2343 t
ORIGIN      
        1 gaaaatttac atataaaact aaaaaatgca agtaattgac tttttaagtt gacaaaatat
       61 tgcatatata ttatgatata aatcataaat taactacaaa tcaatattat atattcgtag
      121 aagaagaaag tagcataaag gaattttaca gagagttaga tttggctgag aactaatatt
      181 ggaatttatg tgaaaaggac tttggagaat actattgtaa ttatgttaga atagtggttt
      241 aattcgcctt aagaaaatga gtgagtaaaa ttaattttta ctaataagag tggtaacacg
      301 gatttatatt cgtctcttgg atttttccaa gaggcgtttt ttattataaa cttatttcag
      361 aaaatttaaa actatatatt aaatgaaaat tataaaaata tgatagttaa taaatttata
      421 aattagaaaa ttgtaaaata tgctaaggtc agtagtttta cgatttaaat ggagggatga
      481 aaatggaaga agaaaaaatt aactatgctc tggaacagat tccagagaat caaaaacgtg
      541 gttgggtagc tatgttttca gttttagtag caataggtgt ggatttatca tctgttattt
      601 taggcgctga gttggcacag agtatgccaa tgaaacaagc aatattgtca gtaattgttg
      661 gttcattttt ttctgcaatt ttgtatacta catgctcttt ggttggttca tctacaagtt
      721 tatctacatc tatgataaca aagtatgtat ttggagaagc tggagcaaaa atattttctc
      781 tagttatagg ggtttctcta ttaggttggt ttggagttca agtaggtttc tttgctcaaa
      841 atgcacagat aataataaaa gatatattta atttagatgt cagtatgcag attttaagcc
      901 taataggtgg tctcttgatg atgagtactg caatttatgg atataaggca atggagaagt
      961 tgagtgtgta ttcagtacca tttttattag ttcttatgat gctaactata ttcttagctt
     1021 ttagagcaaa tggaatttca gttgatgata atatgaaatc tactatgaca tttgcaggtg
     1081 gagtttctct gtcaatgagt ataataatag ttggtgcaat tgtatctcct gatataagtc
     1141 gttgggctaa aagtagaaga gactgtgctc tttcaagctt tttgggaata caatttggaa
     1201 atgcttttat gataatagtt tcgatagtat tagtaaaatg tatgggaact tcagatatta
     1261 tgagaatttt tattacatta ggaattgcta taccaggtat aatagtactt acattagcac
     1321 aatggactac aaatacaagt aatgtatatt cagcatcatt aagtatagct cttgtactta
     1381 agaaggctcc tgagaaagta ttaactatag tccttggtat tatagcaaca ttattggctg
     1441 tatttggaat atatgaaggt tttataggat ttttaaattt acttggaatt gtaattgcac
     1501 ctgttggtgg tgtatataca gcagaatatt acatagtaaa gcaagaactt aaaggctttg
     1561 ataaaggagt attatataaa ccaatagtta aacgttcaat agtgtcttgg ataattggta
     1621 tattaataac ttacctttca acatatggat ttataactct tactactata gcacctttag
     1681 atggttttat agcagggttt gtagtacaat ctattattgg aaaagtgctt tgttcaacaa
     1741 aaaataagca agaaatagac aaagcagtat aaagaaattt gtttataaag tataaaataa
     1801 attagaaata tgaggagaaa gtaacatgag atttttaaca gaagaaagtg tagataaaat
     1861 agcagttggt gcagcagtgc ttggaactgg aggtggaggt gacccatatg taggtaaatt
     1921 ggttgcaaaa caagccatta aaaagtatgg tcctgtaaaa gtaataagtt tagatgaact
     1981 tgatgatgat gctttggtag ttccagtatc tggtatgggt tctcctgtaa ttacaataga
     2041 aaaattactt tcagaagtag agttaactac ccctttagaa ataatggaga aattattaaa
     2101 tagaaaagtg gatgttataa ttcctataga aataggaggg ataaactcac ttatgcctat
     2161 agcagttgct gctaaaaaag gacttcctat cttagatgca gattctatgg gtagagcttt
     2221 ccctgaagct caaatggtaa ctttttatct tgaaggatat gaggcatctc ctgccgtaat
     2281 gtctgatgaa aaaggtaatt ctgccatcct ttatcctgtg gatggaattt ggtcagaaag
     2341 attagctaga actttaactg tagaaatggg aggaagttct tctattagtg actataattt
     2401 aagtggagct caagttaaaa aagctgctat aggtaatact cttacaatag ctgaaactat
     2461 aggagggttt ttacttgaaa ataaatctga ttcaaaaggt gctgttgaga atatactaaa
     2521 agaattgtgt ggatacaagt tatttgaagg aaaagtaatt gatattaaga gagagttgaa
     2581 aggtggattt actagaggac atgccttttt tgctgggatt aatgagtatg atggagagta
     2641 ttctatatta ttccaaaatg agaatttaat tgccaaaaaa ggtgatacac cactttgtat
     2701 tacaccagat ttaatagcag tattagattt agaaactgga tttccaataa ctacagagag
     2761 aataaaatat ggctctcgtg ttatggtagt agcttttcct tgtaatgaaa aatggagaac
     2821 agaaaaagga attgagacag ttggacctgg atactttgga tatgatgtgg aatataaaac
     2881 agtagaagaa ttacaaggaa aataaaagtt taggaggaaa ttttgatgta tcgtgtagga
     2941 attgacgttg gaggaacaaa tacagatgct tgtatactgg atggggaatt aaaagtaatt
     3001 cattcagtta aggtggcaac tacaaaagat gttgaaactg gtgtttacaa tgccttaaaa
     3061 aaagttatag atgaaagtaa gattgaccat tcattaataa aatatgctat gcttggtaca
     3121 actcattgta caaatgctat tgtagaaaga aaaagactta ataatgtggc tatgataaga
     3181 ataggaaagc cggccactac tgccattctt ccatttatag attggccaga agacttaaga
     3241 gaaaaaatag aattagattc aatattggta tctggtggat atgagtttga tggcagaaaa
     3301 ataaatgaac tttgtaaaga tgaagttgta gagttttgca atagaataaa aggcaaagtg
     3361 gaaagtgttg caataagtgg agtatttgct ccagtaaata aacaacaaga agaagaagtt
     3421 gcaaaatggg ttagagaaat tttaggggat attcctatat ctttatcaaa tgaaataggt
     3481 aatataggta tattagaaag agaaaatgga gctattttaa attctgcatt gtctcaagta
     3541 ggtaaagctg tatctttagg atttgaaaaa gcacttaaag atttaggtat agatgctcaa
     3601 atatatttca gtcaaaatga tggaacatta atgaatttag agtatacaat gaaataccca
     3661 atttttacaa ttggatgtgg aataacaaat tctataagag gagcttcctt cttatcaaaa
     3721 gtaaaaaatg caatagtatt agatgttggt ggaactacaa gcgacttggg tgtactatat
     3781 aatggattcc ctagagaatc atctattcca gttacaattg gaggagtaca tacaaacttt
     3841 agaatgccag atgttttaac tattggtctt gctggtggta catgtgtaaa aggcgaaaaa
     3901 gataatataa agataggtcc tgaaagtgtg ggatatagaa taactgaaga tgcaataata
     3961 tttggtggag acactctaac tttatcagat gttgttacaa aattagatat ggcgtttgaa
     4021 gatagaaaaa gcaatgttga aaacttagat aaagagtatt gtaatgaggt atataagaag
     4081 gttattttaa aattagaaga tgctgtagac caaatgaaga ctagtcaaga tgatgttgaa
     4141 ttggttttaa ctggtggagg aagtataatt gttcctgaaa aactaaaagg cataagcaat
     4201 gttataagac ctcctcatta tactgctgca aatgctattg gtgcagcttt aggacaaata
     4261 agtggagatg tagaaaaagt atacagttta gataaaatgt cgagagaaga tgccatagaa
     4321 gatgcaaaaa aagaggctgt aaataaagct ctaaaagctg gtgctaaaga agatactata
     4381 gaagtattaa atatagaaga tgttccactt gcataccttc ctggtaatgc acttcttata
     4441 aaagtaaaag ttgttggtga tttaatttaa agaataaata taagtatttc cagaattata
     4501 taaatatttc tggactcatt tttaaactta ttttatcaat aaaaacagca taacttttga
     4561 ctatattctt ataagtttcc gaacagccga tatataccta ttttttgagg tttatcggct
     4621 gtttgttatc ttttatcgca ttccaactat ttttcttacc gtatttgctg tacgaattgt
     4681 aatcttatca cttacatttg aactggcagt ctttgaccac caatttgtct ttttataatc
     4741 tttacgacta aaagaccaaa agatagcatt tttataatga tatacttttt catactcttc
     4801 tttaggattt ccaacctcat cataaaactc gtcataagat aagtcaggaa acataaaaat
     4861 aagattatca tatatttctt tgttgttatc tccccaccaa ttaggagcat tatttagtat
     4921 atcttttaac tcttcttgtg aaataataaa aactggtatt tccaattcaa actgtttttt
     4981 tatcattaat cttattgtat ttgagataac cttgctgtca tctacatcac ttaaaaagac
     5041 aacattacca ctattaaggt atgtagaaac ttctgaataa ccaagttcta caaagcttgc
     5101 ttttaattca gacataatta tcttgttctt accacttata ttgatacctc ttaataatgc
     5161 gatataactt ttcatataat aatttcacct ctaaacattc acatttttct atgtagtaca
     5221 aacttgaatt tgcatctttt ataattcttt caaaacttct tcatatgtaa tttttttaat
     5281 ataaaaaaat cggtgcttaa tttgtaccga tttttgtttt tcctagtaaa gaactcgttt
     5341 ttttgcttct gctatagctc tagcagcact ttttgcttgc attctttttt cttcttcttt
     5401 tttaaattca tttattgttt catttagatt cttatcatgt tcattttttt ctttatcttc
     5461 gttttctctg tatactaata tataatctat tattgattcg tttattatct gagatatact
     5521 tttattttca ttaattccta ttctcttaac ttccttaagt agagtctcat caatatgaac
     5581 cgttgtttgc tttgtatttt ttgccacctt aatcacctct taaaaaaaat tctaacatta
     5641 aaattttttt tagtcaataa attttaatat taaaattctt taaatgtttt atttgatttt
     5701 ctaaaatttt ctattgtatt tatcatctta ttttttatat tttcacctag tacagaagac
     5761 tttagtctag gattttcgat atggtcataa attggttcta aaaattcaac ttcgacagga
     5821 acagagttaa ttttaccaat aggttggtat ccttcataag tatcttttga attttttatt
     5881 acgagtggaa caatagggca ttttgcttta tatgctatct ttaaagctcc acttctaaac
     5941 tccgaaacca atgaatttgg ttctttaatc catgttaaat ctccttctgg aaacactgcc
     6001 atactttgac ctgtaagtat attttgagag gcttgggcta tactctttat accctcacga
     6061 ttgttttttc tgtttacata tacacatctt agtaattttg cccattcttt aaatataggt
     6121 atatttctcc aaactggttc atctgcaatt acacatccaa ttggtctttc tacacttgct
     6181 gttagtatga agctgtcaag catactagaa tgatttacaa caaatagaag tggttctttt
     6241 ggcagtgttt ccttaccaat tatattaagc tctatattca ctatatctaa agatttttta
     6301 gcttgttttt gcataaattc aaatttttcc tttaagctaa atttatcagg attcttttta
     6361 attttaataa gcctaggtat agaagatgta aaacctataa gttgatatgt agcaaatttt
     6421 aagtaattat tcataaaatt atctccttaa aataaaatta tttattactg taaagatttt
     6481 aacataaaat aaaatttaag tctattttaa ttcaattttg taacatatat tttaaataga
     6541 cagagattga gattttatat ttccaatttt agtaatcaga ttttatcaa
//
LOCUS       DAYATN010000181         6458 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.185, whole genome shotgun sequence.
ACCESSION   DAYATN010000181 DAYATN010000000
VERSION     DAYATN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.185"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(372..779)
                     /locus_tag="ACGI8A_003430"
     CDS             complement(372..779)
                     /locus_tag="ACGI8A_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HFH0181394.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1253..2137)
                     /locus_tag="ACGI8A_003431"
     CDS             complement(1253..2137)
                     /locus_tag="ACGI8A_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0181395.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2428..2904)
                     /locus_tag="ACGI8A_003432"
     CDS             complement(2428..2904)
                     /locus_tag="ACGI8A_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HFH0181396.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3170..4345
                     /locus_tag="ACGI8A_003433"
     CDS             3170..4345
                     /locus_tag="ACGI8A_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HFH0181397.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4426..5229)
                     /gene="proC"
                     /locus_tag="ACGI8A_003434"
     CDS             complement(4426..5229)
                     /gene="proC"
                     /locus_tag="ACGI8A_003434"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFH0181398.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5269..5859)
                     /locus_tag="ACGI8A_003435"
     CDS             complement(5269..5859)
                     /locus_tag="ACGI8A_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFH0181399.1"
                     /translation="MVLNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLT
                     SVATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLK
                     EKTMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYN
                     SEKNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2129 a         1098 c          725 g         2506 t
ORIGIN      
        1 gtaagcacag actatatctt atccttcagc attatccgtt aaggtctacc cacttccact
       61 accaatagct tgtagtgtac ttccctcaag aggaatagtc gttgaagttt ctcctattcg
      121 gagtttacct gctgattgcc cattttaaag tacttaggat ttaaccttat accatctaac
      181 caattttttc tactttcgta actttcacac ttaggtttat ttcatcctta tgttttagtt
      241 tgattagctt taggggtttc cagcaattca agtagtattg gataggttct atcctatctc
      301 tacatatagg tttccctata tgctgactat tttagctact aactcatgac taaagtcacg
      361 agtgtgcgta gctatttttt aatcaatatt ttagtaaatt ttacggtaag taaaaacctc
      421 tctccttcaa atcctatagc aaatatactt acaagttgct ttgagaaaat aactcctaat
      481 atagctacaa ctatgcatat accaactatt atatttaaaa cattgtttaa aaactttagt
      541 gcttgctttt caccttgttt actacttata tcttgataca ttggaataaa agtagtaact
      601 atagcagtac ctattgctgc aaatataata tttggtatat tcatagctgt taaataactc
      661 tctgtatata agcctgtacc ataaattgat gataatacta attctctacc cattccaaga
      721 atcttagaga ttatagtaac tatcatcaaa taaaaagttg ctttagctac cttgctcatt
      781 ttatcactcc acttctaaca tacctctttt taatcaagta aaatttagct cattagatat
      841 aattctatca actaatttgc cttgcttagc cctatcaaaa tataattaac acctatttct
      901 taatatatca caaactacat tattcttcat atttttttat ttctccaata agttttacat
      961 ttttcctata taaattgtat agcttcaatt atattcaaag aaatggagtt gctacccgat
     1021 aactttaaaa gtaaaaaaca gattttatta tctttttaaa ctatattcta acatattttg
     1081 tccatattga gaaattttac taaactatta tgtactttat taaataaata aaataacagc
     1141 acacctataa aatacaagtg tgctgttcat aaacagattt tataaaaaaa taattcctaa
     1201 gtagagatta acatgctttc tcatactctc atatataatg tgtctaaaaa tattaataaa
     1261 aatgctttaa ttgttttaac ttacttggat gtcttaattt tcttattgct ttagcttcta
     1321 tctgtcttat tcgttctctg gtaactccaa atacctttcc aatctgttct agtgttttag
     1381 ggtcatcatc atcaattcca aatctcattc ttaaaacttg ctgttcttgc tctccaagac
     1441 ctgtaagtag ttcttctatc ttttctttta aattatgctg ttcaacttcg tgtattacaa
     1501 catctttaaa atcagcatct gatggtataa attcaactaa actactatcc tcatcttctt
     1561 ttaatggagt atccaaagat acaacatttt tatctcttct taataattcc aacactttgt
     1621 caacttctaa gccacatgca tctgcaatct catctatagt tggctctctt aataatacat
     1681 ttaaaagttc ttgtttcttt ttctttacaa aatttattct ctgatgaaga tgtataggta
     1741 tcctaatagt attttcgcaa tcgtcgatat atcttgttat actttgtttt atccaccaag
     1801 ttgcataagt gctaaatttg tatccttttt tataatcgta cttttctaca gcttttataa
     1861 gtcctatatt tcctgcttga attaaatcca acatatcaat actatctctt ttatatctct
     1921 ttgctatgct cactaccaac ctataatttg aatttataaa tttttctttt gctactgaag
     1981 atgagttgat tatttcctta gccagctcta cctcttctcc tgctgacaac attttgtatt
     2041 cctctatttc ctttaaatac attttcatcg cattcgatac atttgatgaa cccacacaaa
     2101 tataattttc ataattattt tcttttttag tatccataat caaacccctt ctattcatag
     2161 aatttgctaa aacaaaaata aataatttaa acctacataa tctacaatta cataatatcc
     2221 cattctttaa atgaattgtt ttgtatatta tgtatacaaa tttataaaaa ctatacctat
     2281 aatattattc tacttctaac agcaatatcc tccttatata tataatttat tttattatat
     2341 gttgttatat tcttagtttt ttgaatgata tatgtctaaa aaaagactgt ttactaaaca
     2401 gtcttttttt atatatatgc tcaatattta attttcatga tgatgattac atccacatcc
     2461 acaattttca tcatggtgat gatgttcttc taaatctttt acctgttgtt ccatttcatg
     2521 aaactctttt accattatat cataagttgg aagagctgcc tcagtatcgt attcttcccc
     2581 tctccaatca gcgtacatca tagcatctcc attagttaaa accaatctac ctgacatagt
     2641 tatattatcc acttcttcta aaaattctat ttgtttttgt aaaacttctc cttctaactc
     2701 cacatcttca tctaaacatt gcactatcat tacaggtgca taataatctt cttcatcttt
     2761 aacatttaca acaatatcta aaattccttc tgcaccttct tcaaattctg ccaactcaac
     2821 atttgtatca atcatatctt gaggtactaa taaatcttct attaaatatt ttattactct
     2881 atcttttact aattcttttg acatttattt ttctccttcc gattctataa cattatcata
     2941 atcatttcta tattatagta tactacatta attgtttttt caatctctta caaaaattat
     3001 tttttattat cttccttata ttcccttttt tattacaaag ttgtaatcgc attgatttaa
     3061 aaaatagttt atatttagta taatagaaat gtagcacata tttttattca atatattaat
     3121 aaaaaaatat attgaaaatg taatgtatgt taaatctagg aggaatatta tgaagtttaa
     3181 aaaacaaatt agttctattg ttgtgtctac tatgcttgta cttgggagta tgaacctttc
     3241 ttatgcagac ggtacaaatg tagttacact tggagctaac ttatcagagt ctcaaaagca
     3301 gcaaatgctt aattactttg gagtaaaaaa agaccaagtc ttagttttag atgtaacaaa
     3361 tgcagaagaa agacagtatt tacaaggtgt tgcaacagag ggtcaacttg gaaaagttac
     3421 tatctcttgc tcatacgttg aacctactaa aaaaggaaat ggaattaatg ttaaaacagc
     3481 aaacttaact tgggtaacta gttcaatgat tgcttcaacc cttacaacag ctggtcttga
     3541 agatgcaaat gttattgctg ctgctccata tcctgtaagt ggtactggag cattgacagg
     3601 aatcatgaaa gcatttgagg atgcttcagg aaagaaactt gatgaaacta agaaagaaat
     3661 tgcttctgaa gaattggtag ttacaggtga cttaggagat gatatagggc aagaaaaagc
     3721 aacaggtgta atgaatgata taaaaacaga aattgtaaag aatggtacaa aagataccaa
     3781 tcaaatagca aatacaataa ataatgttgt aaataattac aatattacta ttacaccaga
     3841 acaaaaagaa caaattaaag gtgtaatgaa aaaaatagct gaccaaaatt acgattacaa
     3901 tagcatgaaa aatacactag ataatgtatc agataatgta aatgaacaat taaagaaatt
     3961 aggtgaaagt gttaagggtt ctggtatact agatactatt ggtggatggt ttagtggaat
     4021 aggagactgg ttctctggtt tattctcagg cggagataaa aaagatttgg gaatcttaaa
     4081 taatactaat gatgagtctc ttggctctga tgctgtaata aattctacag aaggtgttca
     4141 aataaatcca caaaataata ctaattctaa caatgatagt gatacaaata gctcaaaaaa
     4201 tgatacttca agtaataatg aaaataacga tagttctcaa acaaatgaaa acaacacaac
     4261 aaataattca gaaaatcaat caaataataa tgactcagtt ggtttctttg aaaaaataaa
     4321 aaattggctt tcaggcttat tttagaaatt aaacaaataa aaagaattgc ctaaaatgga
     4381 aattttctat aaaaggcaat tctctttatt tacaataaat ttttattact cactcatttt
     4441 tttagatttt tctatacatg cacatattgc ttttatcaca gaagctctaa aaccttcttc
     4501 ttcaagtact ttaactgctt ctatagtagt tccaccaggt gaacatacca tatcttttaa
     4561 ttcaccagga tgttttcctg tctcaagtac catttttgca gaacccataa cagcctgtga
     4621 tgcaaactta tatgcttgtg gtcttggcat tcctgcaatt actgctgcat ctgctatagc
     4681 ttctataaat aaaaatacat atgctggtgc agaaccactt gcaccaatta cagcttctat
     4741 aagatactct ggtactacct cagtatttcc aaaagaatta aatacttctg taactgcttc
     4801 taagtcttct ttgtttatat ttttatttat cgaaagagaa gacatggctt catttacaag
     4861 agctggagta tttggcatag ttctaactat ttttttatct tctcctataa tagactctat
     4921 atcttttata gattttcctg ctgctattgt aactataatt ttattgtcat ctataaagtc
     4981 ttttatctct tccaaaatat catcatatat atctggttta acagctacaa ttactatatc
     5041 agaggttcta gttacttcct tcgagtcttt tgtagtattt attccaaaat ttgcatctat
     5101 tctatcgaga gttccttgac tatatgctga tgctataacc atattaggtt caactaattt
     5161 tgaatttact attcccccta tcatagcgct tgccatattt cctgcaccta tgaatccaat
     5221 ttttttcact ttagtacctc cttattgact aatagtaatc gttaaaattt aagcttcttt
     5281 aactgcatca gatttttcaa atggatttgg tatacttact accaaaattt tcactacctt
     5341 attagtatag ttgacccaat tatgattgtt cttttcacta ttatagtgta ttgtgtctcc
     5401 tggatacatt tctatttgtt catctcccaa aaacactgta agagtaccct ctaatacata
     5461 cacaaattcc tcaccttcat gtacgtaaca acaaatttcc tcactactct tacttggaag
     5521 aatttcaatt aatcttggta gcattgtttt ttcctttaaa ctagatgata aatgataatg
     5581 tataaatgtt gagttttcta catcaaatac ttctttttca taacttctta atacagctct
     5641 tttatgctct tttggcttca taaaaaagta agttaattct acatctagta cacttgctat
     5701 ttttcctaag gaatctgttg ccacacttgt taatcctctt tcaagttgtg ataaaaaacc
     5761 aattgacaag tttgtttttt cactcatatc cttcagcgtc atttgctttt gagttctgag
     5821 ttgctttatt tttgctccta tgtccttatt caaaaccatc acccctaaat aattttaatt
     5881 ttttcataat ataaatacaa aaatataaat ataggtactg aacccatctt tatttcattt
     5941 aaaaaaattt ttataagtat tactataatt tatcaatctt tttttgtcta catttttacg
     6001 attatttacg atttattttt cacatatgta tatttaatct tgccaactaa agatacattt
     6061 tttataagca tttactcttt atataataaa acttaagttg ttcatgtagt cttcttctgg
     6121 tttaattatt aaaatttcaa attatattat tatttctaca cattattaca tcctttagta
     6181 caattataac ataattttac aatttaatga tatctattca atgtcttttt ttactctaca
     6241 acatatttta ttttagagtt taatactata tatatttttt atatacagcc ttgtcatttt
     6301 ttagattgtc aagagttatt gaagtatttt atgtagattt agattttatt tcctaatttc
     6361 tcatttaaca agaattgctt atgataaaaa ataatatcat ctcaataaga ttcttgtaaa
     6421 tatatctata ttctaaatat aatttctttt tttgtcaa
//
LOCUS       DAYATN010000182         6369 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.186, whole genome shotgun sequence.
ACCESSION   DAYATN010000182 DAYATN010000000
VERSION     DAYATN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.186"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            264..1208
                     /locus_tag="ACGI8A_003436"
     CDS             264..1208
                     /locus_tag="ACGI8A_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFH0181400.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1230..1856
                     /gene="hisG"
                     /locus_tag="ACGI8A_003437"
     CDS             1230..1856
                     /gene="hisG"
                     /locus_tag="ACGI8A_003437"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFH0181401.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1890..2939
                     /gene="hisC"
                     /locus_tag="ACGI8A_003438"
     CDS             1890..2939
                     /gene="hisC"
                     /locus_tag="ACGI8A_003438"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFH0181402.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2942..3520
                     /gene="hisB"
                     /locus_tag="ACGI8A_003439"
     CDS             2942..3520
                     /gene="hisB"
                     /locus_tag="ACGI8A_003439"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFH0181403.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3522..4127
                     /gene="hisH"
                     /locus_tag="ACGI8A_003440"
     CDS             3522..4127
                     /gene="hisH"
                     /locus_tag="ACGI8A_003440"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFH0181404.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4124..4846
                     /gene="hisA"
                     /locus_tag="ACGI8A_003441"
     CDS             4124..4846
                     /gene="hisA"
                     /locus_tag="ACGI8A_003441"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFH0181405.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4828..5586
                     /gene="hisF"
                     /locus_tag="ACGI8A_003442"
     CDS             4828..5586
                     /gene="hisF"
                     /locus_tag="ACGI8A_003442"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFH0181406.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5614..6282
                     /gene="hisIE"
                     /locus_tag="ACGI8A_003443"
     CDS             5614..6282
                     /gene="hisIE"
                     /locus_tag="ACGI8A_003443"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFH0181407.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDVIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2598 a          537 c         1260 g         1974 t
ORIGIN      
        1 tgggaggaaa ttatcatggt gagattaaat atagtaaaat taaatacaaa attaaatcat
       61 caccatcatc atagattaaa gggattgtaa tatgaaatta attcatagac acaagccctg
      121 tttttagtgt aattggtctt gtatctatga tgggaagtaa ttttaagaga aaattatagc
      181 catataggta taaagcctat gtggcttttt tattgtttta aatttgcaaa tataagagta
      241 aatttaatga agagggtgag aatgtgaaat ttttgaggat tgaagatgag gttatttatt
      301 ctaaaaaaag atataaatta aaaagggaaa tagataaact ttttttggat gaagaatatt
      361 ttcaaataga accacaatta tttgaagaat atgatgaatt tacaactata aataataaaa
      421 ttccagaaga atctatggta aaggttgtaa atggaaaagt aatggtactt agagctgata
      481 taactacaag tataataaaa agccttgtac caagatggga agatggttta aagcttaagc
      541 ttttttataa ttcatcaata tataaaaata aaaatacagt tggaataaaa gagataagac
      601 aaataggttg tgagtatcta ggagaagcct ctgtagaagc agatagagaa gtagtgaaat
      661 tagctcttaa gatacttgaa aaatacaata ataattttat tctagaggtt gggagtagca
      721 agtatattca tggtttgtta gaagaattaa atttaaataa gaattgtgaa aatcaaatta
      781 aaaatttact atatacgaag aacactcatg aactaaaagt atatattgaa gaattaaaaa
      841 ttaagcgtga agtaaaagag ttattatcaa atattttaag tttacaaggg aatttatata
      901 atgttattga aaaatcaaac aagttctatt gtaataataa gatgaagcaa gctcttgaag
      961 aattaaagca agtaaataat ttaattgagg aatgtaattt tttagataag gctagatttg
     1021 atttatcaat gattactatg cttgactatt atgaaggaat tatgtttaga ggatactacc
     1081 caaactcata taaagaaatt cttagtggcg gaagatatga ttctttaact aaagagtatg
     1141 gaaaagaaat tccagccatt ggatttacac taagtgttga tgagttaatg aagtatgtat
     1201 ataagtaaag tttagaagga gggattgctt tggattattt gactatagca ctagcaaaag
     1261 gacgaattga aggggaatca tttaaaaaat ttaaaaaaat gggtctagga gacagtatag
     1321 acacagatac gagaaaacta atttttaaag atgaagaaaa taaaattata tatatacatg
     1381 taaagccatc agatgtagtt acatatgttg aaaaaggagt cgctgacctc ggtatagcag
     1441 gtaaagatac tattttagaa aatgagacag atgtatatga aatatatgat ttaggttttg
     1501 gaaaatgtaa atttgctgta gcaggattaa agggagatag catatataga gaagatgaat
     1561 atttaaaagt ggcaacaaag tatcccaata tagcaaaaaa gtattttaaa gagaaaggtc
     1621 aaaaaattga aataataaaa ttaaatggtt ctgtagaact agctccaata gtagggttgt
     1681 cagatgtaat tgtagatata gttgagactg gcaatacact taaagcaaat ggtcttgaaa
     1741 tattagaaga catatgtaat ataagtgcta gaattatatc aaatagagct agctatagat
     1801 ttaaatatga acaaattcag aatataattc gtttatttga agaacttgat aattaaaata
     1861 taattgagct ttataaggag ggatttaagt tgagagaaaa agaaagcata agagagttaa
     1921 ggggatatga accaaatcac gtaaactgta aagtaaaact tgatgcaaat gaaggaagta
     1981 agaggttgtt taaatacctt attaaagaaa tttcagatag tgatatagat ttaaatttat
     2041 atcctgaaga ttcttatagt gatttgaaag aatctataat agattacata aatatttctg
     2101 gagtaaataa gaaaaatctt ctagtaggaa acggttcaag tgaaataatt gatttaataa
     2161 tacatacttt tgtagataaa gatgaagtaa tattgagttt ctctccaagt tttagtatgt
     2221 attctatata tagtcagata aatgggtcta aatttatagg tgttgaaagt gatgaaaatt
     2281 tagttataaa tatagataat gtgattgaaa aagtaaagga gaataatcct aaaatagtaa
     2341 ttgtatgtaa tcctaataat ccaactggaa caatattaaa gagggaagag ataataaagc
     2401 ttttagattc aactaatagt ctagtggttt tagatgaagc atatatggat tttggagaag
     2461 aaagcatgtt aagtgatgtt tttaagtatg ataatctaat agttcttaga actttatcaa
     2521 aggcatttgg attggctgga ataagaacag gatatatgtt atctaatagt agtttaataa
     2581 attctgttga gaaagtaaga cctccatata atttaaattc attatcagac tttattgcta
     2641 caagagcact tagaaataaa gatgtggtaa aggcttatat aaaggaagta aaagaggaaa
     2701 gagaagtttt atatgaagaa atgattggta tgggaattaa agcatacaaa tcacaagcaa
     2761 attttatatt attttattct gaaatcgaaa atttatcaca aaaacttatt gatagaggtg
     2821 ttttaataag aaaatttggt ggaaagttag aaaattatta cagagttaca attggtgata
     2881 aagaagaaaa ttctatgttt gttggagcta taagagatat tttgaaaaag gagaaatagg
     2941 tatgagaatt tggaaggtag aaagaaatac tcttgaaaca cagattttag tagaattaaa
     3001 tattgatggt tctggtaaag ctgaaataga tactggaata ggatttttag accatatgct
     3061 taccttaatg tcatttcatg gcaagttcga tttaaaagtg atttgtaagg gtgatacata
     3121 tgtagatgac caccatagtg tagaagatat aggtatagct ataggtgaag catttaaaaa
     3181 tgcactaggt gacaaaaaag gaatacgaag atattctaat atctatatac ctatggatga
     3241 atctctatca atggtagcaa tagatataag caatagacct tatcttgtat ttaatgctaa
     3301 atttgatacc caaatgattg gtagtatgag tactcaatgc tttaaagaat tttttagagc
     3361 ttttgtaaat gaatcgagag taacattaca tatcaatctc ttatatgggg aaaatgacca
     3421 tcataagata gaaagtattt ttaaagcatt tgcaagagct ttaaaagagg gtagtgaaat
     3481 agtttctaat gagatagcat cttcaaaggg ggttttgtag aatgaatata atagtagact
     3541 atggtcttgg aaatatagat tctgtgtcta ggggttttag aaaagctgga atagaaacta
     3601 aaatttctag tgatatagat gaaataaaac aggcagattc acttatactt ccaggtgttg
     3661 gagcttttag agattctata agtgccttag ataaactagg attgatacca ataataaagg
     3721 agcatgtttc taagggtaaa tttatgatag gtatatgtct aggaatgcaa ttactttatg
     3781 aaaaaagtta tgagtatgga gaatatgaag gattaggact tataaaagga agtattgata
     3841 aactggatat aagtttaaaa gtgccacata tgggatggaa taatctaaaa tttaataagg
     3901 caaatgatga tatacttaaa tatataaatg aagatgatta tgtatacttc gttcactcat
     3961 attatgcaaa ttcatcaaat gaagaactaa ttgcattttc agaatatgaa aagaaaatcc
     4021 ctgctattgt tagaaaaggc aatgtatatg gtatacaatt tcatcctgaa aaaagtggag
     4081 aagtagggtt aaatatatta agagcatatg gggagatgat aaaatgataa tatttccagc
     4141 aatagatata aaagataata agtgtgtaag actcacacaa ggagaatttg acaaagtaaa
     4201 tgtatattat gataatccat tagaagtagc atataaatgg aaaaatgaag gagcagaata
     4261 tatacatata gtagatttaa atggtgctag aagtgaattt ggtgtaaata caaaaataat
     4321 tgaggacata gcaaacaata tagatatacc tattcaagtt ggcggtggag ttagggataa
     4381 ggaaaaagtc aaaagtttaa taaatgctgg tgttactaga gtaatattag gtagtatagc
     4441 gattgaaaat ttgaatttag ttgaagaatt ggtcaatgaa tataaagaga aaattgtagt
     4501 ttctatagat gctaaagatg gaaaagtagc agttagggga tgggaagttg taagcaatgt
     4561 agactcatta acattatgta aacaacttga aaagataggt gtacaaacta ttgtttatac
     4621 agatatatca aaagatggga tgttgcaagg tccaaatttt gacatatatg aaagaatagc
     4681 aaaggaaaca tcactaaatg ttattgcttc tggaggcgta acttctatag aagacgtaaa
     4741 aagactaaaa gctatgaact tatatggtgc aataataggt aaagcgcttt atgataaaaa
     4801 gatagacttt aaggaggcac aacaattatg cttactagga gaataatacc ttgtctagat
     4861 gtgagaaatg gaagagttgt aaaggggaaa aaatttaaag atatagtaga tgttgatagt
     4921 ccagaagtac ttggaaaatt ttatagtgat tgtggtgcag atgaacttgt attctatgat
     4981 ataactgcat caaatgaaga gagaaagaca tctttagaat ttgtgacaaa agttgcagaa
     5041 aatataaata taccattttg tgttggtgga ggagtaaata aattagaaga tttcactgat
     5101 attttaagaa aaggtgctga taaagtttct ataaattcat cagcagtcaa aaatcctgaa
     5161 ttaataaggg aagcttcatt aaaatttgga gcacaatgtg ttgttttatc gatagatgca
     5221 aagaaaaatg aagagggttc atggagcgta tacgtaaaag gtggaagaga aaaaactaat
     5281 ctggatgcaa ttgaatgggc tgttaaaggt gtagaacttg gagcaggaga aatcgttgta
     5341 aatagtatgg atgaagatgg aatgaaaaat ggatatgata tagaattatt atcaaagata
     5401 acttctttag taaatgtacc tgttatagca tctggaggcg ctggaaaaaa ggaagatttt
     5461 tatgaggcgg ttaataaatc caatgtagat ggaattttag cagcatctgt ttttcatttt
     5521 ggcgagataa aaataaacga tttaaagaaa tatttaaaag acatgggtgt agaagtaaga
     5581 ttataagtta aatactaaaa ggagattaga aatatggata ataagtgtaa caatgtatac
     5641 agtgatgaag tagagaaatt tataagaagt ataaagtttg atgataaagg gttagtccct
     5701 gttgttgttc aagaggtagt tagcaaagat gtactgatgt tagcatatat gaataaagat
     5761 gtgataaaga aaaccttaaa agataaagta gcttgctatt ttagtagaag tagacaagaa
     5821 ctttgggtta aaggtgagac atctggtaat actcaaaaag tagttaagat gtcttatgat
     5881 tgtgatgtag atactatact actttttgtt gaacaaactg gtgtagcttg tcatacaggc
     5941 aattattctt gtttttacag agacttgttt gatgatagtt caaaaatgga gcttgaagta
     6001 caaacaaata tattgaaaga attatatgat ttaatcaatg aaagaaaaaa taatccagtt
     6061 gaaggttctt atactaatta tctttttgaa aaaggaatag ataagattct taaaaaagta
     6121 ggtgaagaga gtagtgaggt aattatagca tcaaaaaata ctgataaaag tgaacttatt
     6181 tacgaaatta gcgatttagt atatcataca ttggttttaa tgatagaaaa aggtgttgaa
     6241 attgatgaaa taaaaaaaga attacttaaa agaagaaaat agaataagat agattaatgg
     6301 gtaataagaa aatataccac ttttaataaa aagtggtata ttttttattt tatattttga
     6361 tttatagaa
//
LOCUS       DAYATN010000183         6350 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.187, whole genome shotgun sequence.
ACCESSION   DAYATN010000183 DAYATN010000000
VERSION     DAYATN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.187"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(4..573)
                     /locus_tag="ACGI8A_003444"
     CDS             complement(4..573)
                     /locus_tag="ACGI8A_003444"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFH0181408.1"
                     /translation="MFIVTIQEDFYMIKETHERGGYIFALLILPFINNLYLVKYDIAY
                     KIVLIIIYIYFAYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLI
                     YSFFKSLTIFTNNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIK
                     TNSKTEKFISKLLNFIVIFLIGYQFYILL"
     gene            complement(653..1357)
                     /locus_tag="ACGI8A_003445"
     CDS             complement(653..1357)
                     /locus_tag="ACGI8A_003445"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFH0181409.1"
                     /translation="MKKKILIPVIMSLFIISQCITSFAFTPENNKFKVKPLPYAYDAL
                     EPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVR
                     NNAGGAYNHKFFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWA
                     WLVATKDGKLSIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVV
                     NWNGALENYKNLKSQD"
     gene            1530..3404
                     /gene="asnB"
                     /locus_tag="ACGI8A_003446"
     CDS             1530..3404
                     /gene="asnB"
                     /locus_tag="ACGI8A_003446"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFH0181410.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(3496..4932)
                     /locus_tag="ACGI8A_003447"
     CDS             complement(3496..4932)
                     /locus_tag="ACGI8A_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFH0181411.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            5188..5586
                     /gene="tnpA"
                     /locus_tag="ACGI8A_003448"
     CDS             5188..5586
                     /gene="tnpA"
                     /locus_tag="ACGI8A_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFH0181412.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            5583..>6350
                     /locus_tag="ACGI8A_003449"
     CDS             5583..>6350
                     /locus_tag="ACGI8A_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFH0181413.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNKGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFI"
BASE COUNT         2509 a          742 c          948 g         2151 t
ORIGIN      
        1 caactataat aaaatataaa attgatatcc aatcaaaaaa ataactatga aatttaatag
       61 cttagatatg aatttttctg tttttgagtt agtttttata ggtagtaaag aaatgtttat
      121 ggtaatggga taaaacaatt caataccttc tttagtaata aggtcaagac acaagtgaga
      181 tagatatcct attataaacc cactggacaa acatgagaaa actatattat tgttagtgaa
      241 gattgtcaga gatttaaaaa aactataaat aagtaataag gcaattagac tgtgagtaaa
      301 ccctctatgt ctaaacctac ttccaaataa tttgtatatt aacataaatt ttttacttat
      361 gtaagaacct ctcatatcta tatctggaaa taaagaacct aaatatgcaa aataaatata
      421 aattataatt aatactattt tatatgcaat atcgtattta actaggtata gattgtttat
      481 aaaggggagt atcaaaagtg cgaatatata acctcccctt tcatgtgttt cttttatcat
      541 ataaaaatcc tcctgaatcg tcactataaa cattatattg gtaaatttag actatctttt
      601 attatattat atagcaaaca taagtttttt tcaaaaggat tttaatattt aattaatctt
      661 gagattttaa atttttgtaa ttttctaaag caccattcca atttactaca ttaaaccagt
      721 tgtcaatgta ttcatttctt ctattttgat attttaaata gtaagcatgc tcccaaacat
      781 caagtcctat tataggagtt aggtttttac ttacagggct atcctgattt ggagtagtca
      841 taatagataa tttgccatct ttagtagcta caagccaagc ccaaccagaa ccaaagacat
      901 ctaaagcaga tttttggaac tcttgcttaa atttttcaaa agaaccaaag tctctatcaa
      961 tagcttcttt taaagattca gaaggtatgg ttttttctgg cgtcattata tcaaaaaaga
     1021 atttatgatt ataagctcca cctgcattat ttcttacagt tgtagcaata tctttaggta
     1081 aagaatctaa attttgcaat aattcacata aagaataatt ataaagctca ggatattttt
     1141 caagagcagc atttaattta tcaacataag cttgataatg cttatcatga tgcagtttca
     1201 ttgtttcttt atctatataa ggttcaagtg catcatatgc ataaggtaat ggtttaacct
     1261 taaatttgtt attttcaggt gtaaaagcaa atgaagttat gcactgtgag attataaata
     1321 aagacataat aactggtatt aatatttttt tcttcataaa aatcacctcc taatatagta
     1381 tttctagaaa taaaaaaaat atgaaatatt aacagataat ttataagaat ttaatagcaa
     1441 tttcatttta aattgaataa gttttaaagc caaagacaaa actatgaaat atagaataag
     1501 actaaaggac agaaaggaat tttgattata tggacggcaa tatttcaatt tattataaag
     1561 gtgatatatc taaaaaagaa gatattaaat ttaaaataaa agaatctgta aataatacaa
     1621 gactttctag tagaatagag tataaggatg aggaaaaaac ttttgatttt gaaagattta
     1681 atgtaattga tgctatttat ggaaataaac catttgaaaa agatggaaaa gttattgttt
     1741 ttaatggaaa tatatataac tatcatgaaa taaaagaaga attaattaaa aaaggacaca
     1801 attttactac aaatgatgat attgaagtct tattagcatc ttatatagaa tttggtaaga
     1861 attgtgtaca taagattaaa ggaatgttca actttataat ctatgataga gaaaatgaaa
     1921 gtatatttgg agcaagagat ttatttggga taaaaccact ttattatata aataaggaaa
     1981 atgcaataat tttttcctca gagtataagt ttttacttga atatatgaaa aatttaaata
     2041 taaatgaaag aagcttacaa agttattttt catttcaata tgtacttcct gaagatacta
     2101 tgatacaagg aataaggctg ataccagcag gtcactattt tagggtagaa aatggtatac
     2161 tatctttaaa gagatataat aaacttgagt ttagaagtag tacaaaattt ttttatacta
     2221 aaaatcactt aggcaataga gatgtgaatg aggatgatat aagaaatata gtagtagatt
     2281 caatagtaac tcatatggaa gaggagaaag aaattggaac atttttgtct ggtggaatag
     2341 attcatctat tataactact gtggcttcac aaattaatcc aaatataaaa tctttttctg
     2401 caggctttag tgtcaaagga tatagtgaat tagaagtagc aaagaaaaca gctgataagt
     2461 taggtataga aaatatccag attaatataa cacaagatga atatataaaa tcattaccga
     2521 atgtaattta ttctttggat gaccctatag cagacccttc agaagtaggt ctttatttct
     2581 taattaaaga ggcaggtaaa catgttaaag tagcattgtc tggagaagga gcagatgaat
     2641 tatttggagg atataatata tataaagagt atagtactat gaaatcagtt gtcaattcac
     2701 ctacttatat taaaagtata ttaggaagag tttcagaact tatgccaaac ataaaaggaa
     2761 gaaattatct ttatagagct acaacaccat tagaaaaaag atatatagga aatgcaaaag
     2821 tttttgaaaa cagcgaagta aaaagattct tttttaagta taaggaaaag aatatatatg
     2881 aatacctgtt gtctaatttg tatagagatg cacaaaaaaa taattacgat tatataagta
     2941 aaatgcaaca tatagatgtg aatacatggc ttcaaggcga tatacttcaa aaaattagca
     3001 aactttctac agctgagcag gttgaactta gagtaccatt tttatataaa gatgtatttg
     3061 atgtcgcaaa aaatttaaga atggagcaaa aaataaataa gaataataca aaagttttac
     3121 ttagagaggc gtttagagaa atagtcccag aacatgttgt tcaaaggaaa aaattaggat
     3181 ttccaactcc aataagagtt tggttaaaag attctctagg ggatattgta aaggaaacta
     3241 tttataattc aaatgtagat gaatttatag ataaaaaata tgcaataaaa cttttagata
     3301 cgcatcttaa aggtcataga gataattcga gaaaaatatg gactatattt acattttgtc
     3361 tatggcatca attatttatt gagcataaaa atgtcgagta ttaatacttt tttcaatatt
     3421 aatcagaggg caaaagcatg caatgttttc accctcttga ttattatgga ataggataag
     3481 tagaattttg attgattagt ttactatctc aataaaatta ttggcaaata ttttgacaag
     3541 actactttca acagctaaat ttaagttttg tccatcatca tatccagcgg aaatcaatcc
     3601 tagtaagttt ccaaacatat ctaaaagagc tcctcctgaa ctaccactag agattggagc
     3661 tgtaaattgt atcatttgaa ccgtttcaaa atcacgaaaa gctgaaataa ttccatctga
     3721 aactgtatta aaaagaccta gagggcttcc aattgcaact attttttgac ctctcacagg
     3781 ttttttactg actttaacag gtattggctt gcattttcta tcaaccttta tcacagctaa
     3841 atcataatct gaatggtatt ttataatttg gtaagatgta taaactttat catcattttc
     3901 aaatctaact aaaaatgaat acccatcatt gactacatga agattcgtaa gtatataacc
     3961 ttcattattt attactactc cagaaccgct cttaaacggt ttattttcgt tattgcacac
     4021 aacaatcata acaacagaag ctgataactt tgcaaggtct tcataagaaa gttctacttc
     4081 ctcatcttgt acaatttttt tagatgttag atttgtgtta ttaagtgtat ttttagagtt
     4141 aggtttattc gtagtaggag aagtcttatt ggtattttca ctgtaattta tatttgtaat
     4201 atgcctttca aaaggtattt ctaatatatt tggagtcttc ctagaaagtt caaatagagt
     4261 agaaaatttt gaacattcag gatataaatc aagaatatct ttatttggat ttatatataa
     4321 aatatcacac ccaagttgtg ataaaaaata taagaaaaaa agttcttgcc ttttaatatt
     4381 tccaaagtat actatctttg gtgaaatagt agtcttcacc ttgttataaa ataatttagg
     4441 tagaaaatag tctatccaac acaaaagttt aactgcaaag tttttgatga tagaggatgt
     4501 acaatttggc atacttgtgg aaaataaatc taaagtattt ttaaatgcaa tctttttagt
     4561 ccaatttaag gtgtcatttt ctattttaca tggaaagtcg atattatata aatcccaact
     4621 gttaaactta gaactattat aaatattaaa aattttaagc atcttatctg tttgttcttt
     4681 gtcaaaattt atttttagat tgtttaatct aatataggga attgtaggat tattaaaaaa
     4741 ataagtatct atatttttaa ttttttctat gtatttatca attgaatcat caacacctat
     4801 atatcttgta aagtagactt ctttaatata tgaagaactc ttatttttat aggtagtatc
     4861 agatataaat tcaattagag gttcagtaga cttttttatt gaaatcttta atggtttaat
     4921 agtacaaatc atattttttc tcctataata agatgatata atctattata aaacaactta
     4981 aaacaatata gtacaataat tttgtagttt caaaataaaa tatgtacttg tttatataaa
     5041 tgatatataa ttacaataaa attgccgaaa agccctctcc tttaggtgta gggatgaaag
     5101 gtaaggtctt aatataaaaa aagttagaat aatgcttgta atctaatacc gctactgata
     5161 ccctatattt aggaggtgtt agtttttatg tataaatcta acaaaaatat agtttatgat
     5221 tgcaaatatc atattgtatt ttgccctaag tatcgtaaac ctgttttagt tggcgaagtt
     5281 gaaaagatgt taaaagaaat actaccatat aaagcagatg aacttggagc agaaataata
     5341 gaaatggaaa ctgataaaga acatgtgcac ttattaatat cttgtgaccc tcaatatgga
     5401 attcataaag tagtaaaagg tttaaaaggc tttagttcga gagtattaag agagaacttt
     5461 ccacatttaa agtctagtat gcctagcatg tggactaata gctattttgt aggtactgtt
     5521 ggttctgtga gtttagaagt agttaaacaa tatatagaaa atcaacaagt taggacaaag
     5581 aaatgataaa aactcataga gtgaaattaa acctaactag aacacaattt gaattagtaa
     5641 gggaaaagca aatggaaagt gctaactgtt ggaactacat agttaactta tctaaagaat
     5701 actattttga acataaacaa tggattaaaa agaatgatat acaaaagtta atcaaaggta
     5761 agtacaatct tcatagtcaa actattcaag ctatatcaga taaatttgat gcaaatagaa
     5821 agactatatc agaattaaga aaaaaaggta atactaaagc aaaatatcca tacaagacta
     5881 agaaatttta tataatacct tttaaagcta gtgctattaa tagaaatgct aaaggcaact
     5941 taaaattatc aatgtcaaaa ggcaggtatt tagaattaga ttttaatgtt gagaatataa
     6001 aaactgctga aatagtatgg cgaaatggat attatttata ctacactttt gacaatgaat
     6061 taaatggagt tgtagccaaa ggcactaata ccgtaggtgt agacttaggg gaaatacatt
     6121 cgatagctag tgtaacaaat aaaggtgtag ggcttattct atctaataga gaaggcagaa
     6181 gtattaaaca atttagaaat aagatgtatg cttatatatc taaaagatta agtaaatgta
     6241 aaaaaggtag tagacaatca aagaagttat ggagattaaa aaataagata agaagtaaaa
     6301 cagataatca gttaatgaat ttatatcatc aaacaactag aaagtttata
//
LOCUS       DAYATN010000184         6278 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.188, whole genome shotgun sequence.
ACCESSION   DAYATN010000184 DAYATN010000000
VERSION     DAYATN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.188"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(999..1550)
                     /locus_tag="ACGI8A_003450"
     CDS             complement(999..1550)
                     /locus_tag="ACGI8A_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFH0181414.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1741..2703
                     /locus_tag="ACGI8A_003451"
     CDS             1741..2703
                     /locus_tag="ACGI8A_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFH0181415.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2998..4842
                     /locus_tag="ACGI8A_003452"
     CDS             2998..4842
                     /locus_tag="ACGI8A_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFH0181416.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5164..5748
                     /locus_tag="ACGI8A_003453"
     CDS             5164..5748
                     /locus_tag="ACGI8A_003453"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFH0181417.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKGLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2471 a          600 c          956 g         2251 t
ORIGIN      
        1 gaaatttatg aatattcata ctataattta aatataagga gatgcccttt gaaaataaaa
       61 attaaaaaat atttaatgct actacaatag gaactagaac tacacttaat aaatatacag
      121 aaattagatt tggctcaata taaaatacaa atatagaatt taggtgtttt ttatgaaaaa
      181 atttttatat gctttatata gtttcattgt tattatagct aattttagat taaaagaaaa
      241 aaattataac tttattttat tagcaatctt ttttatattg ctgttaattc aaaatggagt
      301 tttaaaaaca ggttatcaaa atagagctac attttatcaa gataattatt ttaatagaga
      361 ggataaaaat gataagataa aatttatgac tataaagatt tttatatttt tgtcattacc
      421 attatgtttt aatatactat tcaattattt aacctcttaa attatattta gatataggag
      481 tcttatgata aaagtaagct tatgtgttat ttgtagtaaa tataaagtgc atatagtgat
      541 aaaacaaaag tttcatataa atgaagaaaa aaatataaaa taatttattt aactacttaa
      601 aaataaacta aaagggataa taaaatagct tttaggttat ggtaataaag gtatttaaaa
      661 agagtgcata agcactccaa ttgtgagaaa tacatacaca aatcattatt acaacaatga
      721 aaaatgtaca tataagagac gttttgtatt tgttttaagt gataatattt taatactaac
      781 taaataatta tcacaatagt aaaaaagaaa aaggagtatt ttgtactcca tttgtcaaaa
      841 atataaaact tttatataag cttattataa cataattgat aaaagtgtaa agatatataa
      901 tgaatttata aagaaaaata aaataaaaaa gataaatcct aatcaaagga tttatctttt
      961 tgtatagaaa ataattattc tattattaaa tggcatggct aaactggata ataatttatt
     1021 attactgctt tcaatgtttt ttctccttga ttatggtatg tatgaattga ttcagctgaa
     1081 aaacaaatag agtcattttc tctaagttga taaatagagt catttacttc taattttaat
     1141 tgaccttcta taatcataat atattcctgt gatttttcag aatgaccaac agatgtataa
     1201 ctatgacctt cttctaattc catttgaaat aattcgaagt ttctagttgg tgtattggga
     1261 taataacaat aaagacgata atctttattt tctgaaactt gtacatctat atcagttttt
     1321 gatacaataa aagtttcatt ttgaggttgt tccagtattg cagtatatgg aacatttagt
     1381 ccacttgcaa ttttccaaat tgtgtttact gttgggtttg agtctccctt ttcaatttgc
     1441 gaaagcatta ctttacttac gccagataat tcagcaagtt gacctagact taagttacgt
     1501 tcattacgta atctatttaa atttttagca ataacagtat taatactcat agtagctcct
     1561 taaaatgtaa attattgaca aatataattt acgaatgtat aatttattta acattattta
     1621 taatttacaa atgtataata taatgaacgt taaatacact gtacatccgt aatataaatt
     1681 atactgcata aagagttgtt tttcaacgga tgatagttga gagattaagg gggaattgta
     1741 atgcgtgtac ttaataatgt tacagaggct ataggcaata ctcatatgtt acatttatcc
     1801 aaaatagcag atgaatttgg agttcaaggt aatatttatg caaagatgga atacttaaat
     1861 ccgggattta gtaaaaagga ccgtgtagca ttacaaatga ttaccgaagc agaaaatgca
     1921 ggtttattag ttccaggtca agcagttgtg gaattaacaa gtggtaatac tggaacagga
     1981 ctagcaatag cttgtgcatg taaaggatat aaatttatag cgtgtatgtc aaaaggaaat
     2041 tctatggaaa gagcaagaat gatgaaagct ttaggtgctg aagtagttct tgtagaccaa
     2101 atgcctaatt cagttcatgg acaggtatct ggtgaagatt tggcacttgt agaaattgag
     2161 gctcaaaaaa tagcaaaaga aagaaatgca tttagagcag accaatttaa tttagaagct
     2221 tgtaatcatg cacatgaaaa gtatacagca gaagaaatgt gggaacaatt ggatggaaat
     2281 attgatgtat ttgtagattt tcttggaagt ggtagtactt ttgcaggatg ttcaaaaaca
     2341 cttaaaaaac ataattcttc tatcaaatgt tatgttgttg aacctgaaac agcagcaatt
     2401 tattcaggta aagaaataac tgaccaaggg cataaaatac aaggtggagg atattctatg
     2461 gatttgccat tagtagacaa gaaacttatt gatggttata ttcaagttac agacgaggag
     2521 gcaactgatg tagcaagaaa attagctaaa ttagaaggtg tatttgcagg attttcttca
     2581 ggggcaaatg tatgtgcagc aattaagttg ttaaagagca gtgaaaaagg aaaaaatata
     2641 gttttaactc ttaatgatag tggtttaaaa tatttaagta ctgatttata tgaagaattg
     2701 taataatagc acaatagtta tgcaatgttt tttaaattaa gtatatagat ttaaattata
     2761 aaaagtatct caatgtaatt tttgaaagtt ataaaggaga tactttttta ttttgtaaat
     2821 atggagtatc ctacttataa gtattaagct atatagatat tgatgattag ctatgcaata
     2881 aaaaagagta tgtgtttttg catttataca tcataacttg gaataataca tattaatgtt
     2941 ctaaatgtat ttaaaacata tgataaaatt agaacaaata gatacaagga gataaaaatg
     3001 caaaagagaa ttttaagaaa ttttatattt acgggtaaaa atattttaag taaacttgtt
     3061 tttataataa aatataattt tagtacatta attttatttg aattatttca taaaggatta
     3121 gccttatttt ttatatggcc atctattaga tatatatttg atattttaat aaaaagttta
     3181 catatagtct acctaaatat ggataatttt ataaaagttt tgaccaatcc catgtcaata
     3241 gttctgataa tactatcagt tataatacta gctttttatg cattttttga atttacctct
     3301 gtaattattt gttttaataa atcgataaaa tatgaaaaaa tagggttatt tgaactcttt
     3361 aaaatatcat ttaagaactc tataaaattg ttatatccta aaaatatact attatttata
     3421 tttgtgatat taataattcc attagtaaat acagttttaa tttcagggtt tataggaaaa
     3481 atacaaatac cagagtacat actagattat ataaagtctg atataatttt aaatataatc
     3541 tatatgattt ttcttttaat aatatatatt tcggttatta gatggatttt tagcattcat
     3601 gagataactt taaatacaga aaattttaaa agagcaagaa aagaaagtgt taatcttata
     3661 aagggaaaga ttataagaac aattatttat tcattaatat tatctttagt agtagctatt
     3721 attggatatg cgatttatca tagtggaatt atatttatag gcatatggac taaatattgt
     3781 actgatatgg aatttttgaa gaatgtattt ataaatagat gtattgcatt tgaagaatat
     3841 tttagattga ttttatcaat attcattttt atattggaca ttggatttat ctcagcaact
     3901 tattatcaat ataaaggagt aaatatttct aaaaataata ttaaaaaaga gaaaaaatct
     3961 atatcaaaaa ttgtttttaa tttagtatta atattattga tttcttatat agagtcagtt
     4021 gcattttctt cacatataaa tggtatgttt aatacgtcat tttcatttta tacaacagca
     4081 attgctcata gagcaggagc tgaaatggct ccagagaata ctttggcagc aatagaagaa
     4141 gcttcacatg ctaaagctga ttatgcagaa atagatgttc aagaaactaa agatggagaa
     4201 cttattgtac ttcatgattc aaactttaag agaactacag gagtagataa aaatgtttgg
     4261 gatgtaaatt ataatgaagt aaagacatat gatgcgggaa gcttttatta ttatggaaaa
     4321 ggttattatg atgaaaagtt tgttggggaa aagataccaa cactcaaaga aatgataaaa
     4381 tattctaaag gtaaggtgaa attattaata gaaataaaac ttaatggaca tgaaaaggga
     4441 gacgtagtaa aaaaagttgt tcagttaatt aaagaaaata attttgaaaa tcaatgtata
     4501 gttgcatcta tggataaaac tatattacaa aaagtaaaaa aactggatga gaatttattg
     4561 acatgctatt ttatggcaat tgcatatggt gatttttcaa agttaaatta tgttgatata
     4621 tatggaattg aatcaacttt tgttaataaa acagtagtag aaaagattca taaaatgaac
     4681 aaaaaaatct tcgtttggac tgttaatagt gacagtttaa ttgagaaaat gcttgaccta
     4741 aatgtagata gtattattac agataatcca taccttatag agagtgcaat ttattggaag
     4801 aaaaatggat ttattagtca agtgtcagat tatttatttt aaatattcta taattgtatt
     4861 atccagtaaa agagatatta ttaaaatcaa taatatctct ttttaacata aataaggttg
     4921 ttaaattaag gttattctta taaaaattgt gcttatcagc tataaacaag gaataaaagt
     4981 gaaaaaattg ctctgagatt attacagtta aaagagtttt aagatgacag agagatagat
     5041 tgtcaactgt atatttaata aatcttgtta tttattaaat tttatataag taaaatatat
     5101 aaattaataa atcttatgct agaatatata tgaaaaacaa tataaatttg gaggattaaa
     5161 aacatgacag agcgagaaaa aatgttggca ggagaacttt acgattgtgg tgatattgag
     5221 ttgttaactc aatggcataa agcaaaaggc ctagtaagag attataacca aacagattcc
     5281 tcggatttgg aaaaaaaaga aaaaatatta aatgagttac ttggtggtaa gggtaaaaac
     5341 ttatggatta ctgcaccgta ttatgtagat tatggaaata atatttattt tggaaataat
     5401 tgtgaagtca atatgaattg tacgttttta gatgataata aaattatcat tggggataat
     5461 gcactaatcg caccaaacgt acaaatatat actgcatttc atccaactaa tgctggtgat
     5521 agatttggta atgccaaaga agatggttca tttgaatttt gtaaaactca aacagctcca
     5581 gttactattg gagataatgt ttggattggc gggggtgcta ttatcatgcc aggtgttact
     5641 attggagata atgtagttat aggtgcagga agtattgtta caaaagatat tccaagtaat
     5701 atgattgcat acggaaatcc atgccgtatt attcgagaaa ataaataaag ttaaattttc
     5761 ttgcaaaatc tcaaaaaaca cctttcttac tttatacata gggtattttc taatactttc
     5821 acatccatat tcatttgtta aatttttgtg gaatgtagtt tttatcactt ctatcttaca
     5881 tttccaccat ttttttataa aagtcattcc tttatagatt tatttataat agtatatatt
     5941 ctatttgatg tttgaataag cacttatttt taagtaaaat actaactttt aggttagtat
     6001 tttgctatag tatgatgatt aagtaagaat aaaatactat aatacataaa gcttaataaa
     6061 tataattttt attttactta gatattttct tttgattagc aatcaaaagt agttataatt
     6121 tttttaatta ataagagtaa catttatttt atgaaagagg attatattaa tatatattta
     6181 aataggaaaa attgtaaaaa gcataaaaat aatataatta attaatgaga taaaagtgga
     6241 taaaaatatt gggagtttaa tttttgtgaa attaaaaa
//
LOCUS       DAYATN010000185         6035 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.189, whole genome shotgun sequence.
ACCESSION   DAYATN010000185 DAYATN010000000
VERSION     DAYATN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.189"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            2..1312
                     /locus_tag="ACGI8A_003454"
     CDS             2..1312
                     /locus_tag="ACGI8A_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181418.1"
                     /translation="MTTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLYLEIEDR
                     FDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDFSRAYFD
                     LTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHINLWNEGK
                     IDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYI
                     TVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTAC
                     YIKESIENSTNIKVCIGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNENYETIGP
                     LLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELSSCLNVT
                     VRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(1440..2630)
                     /locus_tag="ACGI8A_003455"
     CDS             complement(1440..2630)
                     /locus_tag="ACGI8A_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0181419.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIREETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEVLLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(2736..4115)
                     /locus_tag="ACGI8A_003456"
     CDS             complement(2736..4115)
                     /locus_tag="ACGI8A_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0181420.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(4146..5354)
                     /locus_tag="ACGI8A_003457"
     CDS             complement(4146..5354)
                     /locus_tag="ACGI8A_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181421.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT         2065 a         1087 c          713 g         2170 t
ORIGIN      
        1 cataacaacc gaatttttaa aagagtttgt agataattct ataaaaaagt taaatattaa
       61 tgcagaaatt gaaatatata tctatagaga tttttctcat gttggagatt tatatttaga
      121 aatagaagat aggtttgatg gatttgcagt tagtggcccc atccccaaaa aagctattac
      181 aaaaaaggct ggaactatta aaaaacctct agttgatttt ggtactgact tacaaagtta
      241 ctatgatatg tttctaaagc ttatctttaa atataataca ttagattttt ccagagcata
      301 ctttgactta acagactggg cagataatcc aaaagatata tcatattatt tatcaaatgg
      361 cacatttgga gacttaatgg atagtattga tgaaaatgca tctaaaatga gtttatctga
      421 catagcaaaa cttgaaaaaa agattgcaaa aaaacatatt aatctatgga atgaagggaa
      481 aatcgatttt tctactactc gttttagtag tattgttcct atgttacaaa atgcaggagt
      541 aaactttcat ttcatatacc ctgatataga tttattaaaa aatactttta ataacttaat
      601 aaaagatatt catttacata gaatgaaggg aaatcaacca agtgtaatat atataacagt
      661 taaagataca aattatgatg actctactct agaccaagtt tataaaatat taaatagtat
      721 taaaaaagat aaattaaccg attttataat tgaaaaaaat agtaattctt ttaaaatttt
      781 cactaaatgt gaaacgatta aatcattaac taaaaatttt actgcatgtt atataaaaga
      841 atctattgaa aatagtacaa atattaaagt atgtattgga tatggaattg gtaatgatat
      901 aaagcaagct atgtctagtg ccataagtgc taataaggaa tctaaaataa atcctttaaa
      961 ttacagtttc ttagttaacg aaaattatga gacaatagga cctttactgt gtgataagtt
     1021 acttactgtt tcaaataaag taactccaca tataaataca attgccaaaa gttctaaatt
     1081 gtctactctt actattcaaa agattatatc tgttactgaa attctacaaa gagatgaagt
     1141 tactcctaag gaattatctt catgtttaaa tgtaacagtt agaactgcta atagatttct
     1201 aagtagtctc ttaaagtctg gaaacgctac tatattatat gaaaaacaaa actcaacaaa
     1261 aggcagacct gaaagggtat acaaattatt tatatcagaa aataaatatt aaaatccaaa
     1321 atgcataaaa atattttatc atctaatatt aagtaaaatt atatatgact ttctaaaagg
     1381 tatatctttg catcttttta agatatacct tttatgatat ttaataatat tttatgttat
     1441 tatctactaa aattattttc taaccatttt actgcacaat gagcaaataa agctgaacca
     1501 taaactaatc cctcttcatt aaaaattgct ttaggattat gcataccaaa attatatccc
     1561 tgactaatac ttcccattcc aatccataaa aatactcctg gaacccttag taaaacctct
     1621 gaaaaatctt ctgaacccat tgcagatgga aagtctatta cattttcatt tcctataact
     1681 tctttaacat aagatacaat ctcatctcct acatccaaat catttataac tgcaggagtc
     1741 cccattttaa actccacttt agcatttcct ccaaatgatt tagctattcc tgtagatatt
     1801 tcaataattc tattttttaa tttttctcta acaaattggt caaatgttct taaagttcct
     1861 gacatagatg ctttatttgg tatagcattt ggtacgtttc ctgcattaaa cataccaata
     1921 gttaaaacta caggctcttt ctgaggtttt tctcttgaaa gtatagtgtt taatgctatt
     1981 tgtatatgcg acattatgtt tattgggtcc actccaaatt cagggcttgc tccatgagtt
     2041 gcttttcctt ctactgttat ttcaaatata tccgcagatg ccatagctgg accacgtgaa
     2101 taagctactt gacctgtgtt aagaaattta cccaccatac tgtgaaatgc catagctgca
     2161 tcgacctgag gattttctaa tataccatca tcaatcattg attttgcacc tctggtagtc
     2221 tcttcatctg gttcaaatac aaactttact ctacctttaa gtaagtgctt attatccata
     2281 agtattttag ctgctcctaa gagcattgct gtgtgattat cgtgtccaca actatgagat
     2341 gcatctttat tcttactttt aaaaggtaaa tccgtctcct ccctaatagg aagtgcatcc
     2401 atatctgccc ttaataataa ggtctttcca ccattttcac tgtctaaaac agctactatt
     2461 ccattattag acgtcaattt cggagaatat cccatttttt ctaaagtact ggtaacaaag
     2521 gttactgttt tcggtagatt gcaaccaatt tcgggatttt catgtaaata ccttctccat
     2581 ctaataattt ccccttctat tgactgcgct tctttaaaaa aatttttcat attccttcct
     2641 cctaatcaga tagtcagcaa aatctcactt tcttttagta atgaaatgat gctgatataa
     2701 aaattataat tttatatatt aaatttcatg tctttttaag gagttggaat atcatctgga
     2761 ataggacatt tatatgtttt accattcata gatttatcaa attcttcttt tgccttttta
     2821 gctatctctg gattatttaa tattttagct ccaaacatag ccataatctt tgatgcatat
     2881 atcatacctt tttctcctat agaacttcct gcacaagatg taaactgcca gctatgacct
     2941 ggtgctccta gattcgtaca ccctgtaaaa aagtatgctg tcggaactat atgcattaca
     3001 tctccaatat cagtagagcc ataactatta aagtttgtaa catttcctcc accataatat
     3061 agatgagttc cttctggcaa attatattta cttatcatag ccttatattg ttgtgggctt
     3121 tgtctatcta attcagctgc aaaatccaat tcctctcgag tccaaggctc ttgtttaatc
     3181 tcattcatac attctgatac aagatttgct aaaacatgat tgtttaaagt attataacac
     3241 cctcctaaaa actctacttc aacttcagtt tcagtcatca ttgcagctcc ttttgctacc
     3301 tttacaagtc tttcatatgt actttctaca gcttctctac ttaaagctct tgtataatac
     3361 catactgatg ctttatcagg aactatgttg ggtgctactc ctccatctgt aattgtataa
     3421 tgaatcctta catcagaagt aacatgttct ctcaaataat tagctcccac attagtaagt
     3481 tccacagcat ctagtgcact tcttccattt tgagggtctg accctgcatg tgcagtcttt
     3541 cccttaaagt gaaactttac agagttaaca gctgttgaag taccaattgt agcttcacta
     3601 attttatttg gatgaaaatt tatagctaag tctaaccctt caaacgctcc acctctagcc
     3661 ataaatcctt tacctgttaa ttgttcttct cctggacatg cataaaatac tatagtccca
     3721 cttaagttat tttctatcat ctctctttta agccctacta ctgcccctac atgtgctgta
     3781 cataataaat tatgcccaca tccatgacca tatgcaccca attcaaatgc ttcttgcttt
     3841 gttgaaactt tttgatttaa accaggtaat gcatcaaatt ctcccataaa acctataact
     3901 ggtttacctg aaccaaaact agctctaata gccgtggcta aacctcctac acctacttcc
     3961 acatcaaatc cttcacttct tagaaagttt gctgtatttt cacatgcttt aaattcttta
     4021 aatgcttttt ctggattttc ccatattttc ttacttaaat catcaagttt gaatcttttt
     4081 ttatctatct catttataga tatacttcct atcatttcct attcctccta taagtatatt
     4141 tactatcaac caaatataat tggacctatt atactagcaa atgccactga tgcaatggtt
     4201 acagtaacaa atcctcctat aatcatcttt ggtaataaat aatctgttgc cctttgtttt
     4261 tcttcttcag ttccttccat agcatcaata ccttcgttgg aaattatctg tgttgcagga
     4321 taagctagca tacaagttac accaattgca gctgacattc ttggagaata tcccaatagt
     4381 tttcctacaa ttgcagtaaa taatattata aatacagaac ctacaacaag tattcctata
     4441 gttggaacaa tcatcccaaa caatgctgtt ggagttacct tagcaagtcc tccaggtaac
     4501 atcaacatac aaccaaacat tataattcca ttagattgag atttatctaa aattcctttt
     4561 tctaaaaaac ctattcgtgt aaaaatcaac cctactaaca aacatgctat attaggattt
     4621 aaataatagt ttgcaggggt aaatcctggt attaaagtag ccaatcctac aaaattacca
     4681 atcatagcaa caaatgaaag ctttgcaaga tacactagag tagatgattt tttacctaaa
     4741 tcttctttaa aaattctcat acttggaagt ttaaaattac tttttgactc atctttcaaa
     4801 aaatccccgt tatctctttt aaattttaat tctttattta tacaatatgt agttatcgga
     4861 ataccaaaga acttttgaaa tgataatact agaattacaa atgcagcaag ttcaggtcta
     4921 tttgcagctt ctgcaacagt tgttacgata atcccagcaa cagtagaacc tgctattgga
     4981 ggtgctgcta ttaaagcata ctctctgcca aataacatac tacccactgt taaacagcca
     5041 actcctattc ctacaatacc tactaatgca attacaaccg ttttccattc atgacataag
     5101 tcttcaagat ttataagtgt tcctaagtta gttattaata aagctatacc aaagttgctc
     5161 attgcagcta atatccctga tgctcctgta atgtcttttg gtaaaatatt tgtccaaaat
     5221 cctagtaaaa atataataca tacaaataac aatgttgaaa ttgcaccttt tgttttctta
     5281 gataaaaact cactaaaagc ataaatcatt acacatacag taaagcaagt aagtggattc
     5341 caaaaatttt ccattgttgt ttcctcctaa atataataat tttttatcaa taaaacaata
     5401 attataaaat gtatattatg cagttattct taactacata tggtatagct tgatattttt
     5461 tgatgatatt atatacgtcc taaattattc ctaaaatatc taaataaata taactagagt
     5521 taactctcta aatattaact atagttatca acttactaga tttctttttg atttttactt
     5581 tctacataat tttacgagtt agaattgtac ttcaactact ctctatatta gtatacatgt
     5641 tggaagactt atatttattt aaatattaga tatagaatac atttttataa acgtcttaaa
     5701 ttattcttaa aatatctaaa acgaaataat aatataccca ttaaaccaat attatcagaa
     5761 tatacttcac tttaagtctt ctttatatag aaattttatc tattagtgca tcccctcctt
     5821 tgtaaattag actatttcta aacgaattta gatatcttat tcctaggaac tattactaga
     5881 aacaaaaatt atttattata ttaaatcaat gataagccat actttaaaat tctttctata
     5941 caagtataat ttatcatata atgacatcta tacctttatc tttatagtag tcctaaatta
     6001 ttcttaaatt gacaataaaa ataatttcac tattt
//
LOCUS       DAYATN010000186         6032 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.190, whole genome shotgun sequence.
ACCESSION   DAYATN010000186 DAYATN010000000
VERSION     DAYATN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.190"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(123..323)
                     /locus_tag="ACGI8A_003458"
     CDS             complement(123..323)
                     /locus_tag="ACGI8A_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFH0181422.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            790..2280
                     /locus_tag="ACGI8A_003459"
     CDS             790..2280
                     /locus_tag="ACGI8A_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0181423.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            2560..4056
                     /locus_tag="ACGI8A_003460"
     CDS             2560..4056
                     /locus_tag="ACGI8A_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0181424.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            4190..5116
                     /locus_tag="ACGI8A_003461"
     CDS             4190..5116
                     /locus_tag="ACGI8A_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0181425.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            5234..5425
                     /locus_tag="ACGI8A_003462"
     CDS             5234..5425
                     /locus_tag="ACGI8A_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181426.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            5648..5998
                     /locus_tag="ACGI8A_003463"
     CDS             5648..5998
                     /locus_tag="ACGI8A_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HFH0181427.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         2418 a          616 c         1088 g         1910 t
ORIGIN      
        1 tttaagtttg acgcttttct aaagtaactt cttatttttg taattaaaaa taaatataat
       61 aaacaacaaa aaagagccct cgatagctcc ttttctaaaa tatatatatt taaattaaat
      121 aattatattc tatttacgtt ttcagcttga ggtcctctat taccttgagt tatatcaaag
      181 ctaacctttt cgccttcttc taaagttttg tatccatcat tttgtatagc tgagaaatgt
      241 acgaaaacat catcttctcc ctctacagat atgaacccaa atcctttttc attgttaaac
      301 cattttacta ttccgttttt cattataaga aaacctccgc attattaaaa aatacaaact
      361 aaaaaaataa gtctgttcta ttaatataac ataaatttta aataaaagca aataaaattt
      421 ttaaaaaaat taattacttt tagaatttaa taaaaattgt taaattatac agtgagaagt
      481 attgtaaaat ttgacgaaaa tgtaaatgta gataataaat ttaatatcaa aaaatgtaac
      541 aaaaaggtat gtaagtaaaa agtaaaatta aaatatataa taaaaaatat tatatattag
      601 attgaaaagt aaaaataaaa atagtgaaat ttcagaaata tcaataatat tatataataa
      661 aacaaaaaaa cactatatat attcgataaa ataaaattta aacatataat attttatgta
      721 tatattaata aaaggacata ttgtgttata atggtatcgt ataaaatcat ttacgggggt
      781 ggtattaaaa tgtttcaatt tgaaacacaa atacacaaat taagacatga tgttttaact
      841 gcaatagcta gacttggaaa agaagataac cttaccagag aagcaatgga taatattcag
      901 tatgaaataa taaaaggtga gaaacctaca tacagatgtt gtgtttataa agaaagagct
      961 ataatatctg agagagtaaa tgtaagtatg gggttacaac caggtcaaga taagatagat
     1021 ttagatacaa tggatataga agaagatgaa caaattatat atattttgga agctgcatgt
     1081 gacagttgtc caataaatga atttacagta acagatattt gtagaggatg tctagcacat
     1141 agatgtaaag aagcttgtaa atttggagct ataacacatg tcggaggaat ggcctatata
     1201 aatcatgaat tatgtaaggc ttgtggaatg tgtaaaaaag cttgtcaata tgatgccatc
     1261 tcagaggttg ttagaccatg taagagtgtt tgtccaacta atgccttagg atttgataga
     1321 gaaaatatga aagctatgat acatgaggat aaatgtttaa actgtggagc atgtatgtca
     1381 gcatgtccat ttggagcaat atctgataag agtttgatag ctcctgtagc tagaaagtta
     1441 gttcaaaaag agaaaatgta tgcggtggta gctccagcta taacaggtca agttgaagca
     1501 aatgttacat atggtcaaat aaaaaatgct ataaaatcac taggatttgt tgatgtatta
     1561 gaagcagctt gtggagcaga tgcagttaca gttcatgaaa gcttagaatt tgctgaaaga
     1621 atggaaaagg gagaagcttt tatgacaaat tcttgttgtc caggatttgt tggttatata
     1681 gaaaaaacat acccaaatca tgtagggaaa atatcttcta cagtatctcc tatgatagct
     1741 agtggtagat ttataaaaag tcatgaacca gatgctaaag ttgtattcat aggaccatgt
     1801 acagccaaaa agagtgaggc agctaagaaa gaattaaaag gagctgttga ttatgtactt
     1861 acttttgaag aaataatggc attatttgag gcatttgata tagacttggc taagtgttca
     1921 aatgaagata tagatgatgc atcaattttt ggaagaggtt ttggagctgc tggtggtctt
     1981 acaaaagcta tagaaaatta tcttgctgaa aaaggtattg gtgttaattt tgaacctgta
     2041 aagatttctg gttcaagaga aatcaagaaa actatgacta tggctaattt aggaaagcta
     2101 gaaggtaatt ttatagaagg aatgatgtgt gaaggtggtt gtataaatgg ttctggtaaa
     2161 ttcttatcag gtgcaaaggt taaagccact tttgatagaa aaaattctca atcaactaag
     2221 aaaagtgtct tagcaaatac aaagataaaa gattttagta atataaattt agaaaaataa
     2281 agataataca aagcaacaaa aatacatatt gttaatagag ttgttctaag ttgaggttta
     2341 acttttggac aactctattt ttatttatat atttaattat tgaacaacta aaataaaaat
     2401 tatatacact taaaataaaa attacatata taatattttg tatataactt aacaaagtat
     2461 atgcctatgt tataataatt tatagaaata aataaaaaat acaatataca taatagtaga
     2521 aatatagcaa aaaggtgtga tttataggga ggattactta tgtttgaatt tgaaacgcaa
     2581 ctaaaaaggt taaaacatga agttttaaca gcagtagcaa gactatctat tgaagaaaat
     2641 ttaacaaaag aaaatataga aaaaattcca tatgaaataa ttaaaggaga aaaggcgaga
     2701 tatagatgtt gtgtttataa agagagagaa gtagttcttg aaagggcgca acttgcatta
     2761 agtttaactc caaatagtaa atatggagat ataaatccag attcagttag tttggacaca
     2821 gatgagcaaa ttatatatat agttgaagca gcatgtgata gatgtccaat aaatgaattt
     2881 acagttacag agctttgtag agggtgtttg gctcatagat gtaaggaagc atgtaaattt
     2941 ggggcaattt cttatataaa tgggagagca tatataaatc atgatttatg taaggcttgt
     3001 ggaatgtgca aaagttcatg tcaatatgat gctatatcag aagttgtaag accttgtaaa
     3061 agcgtttgtc caacaggagc tttggatttt aatagaaaca ctatgaaagc aatgatacat
     3121 gaagataatt gtataaattg tggagcatgt atgtcagcat gtccttttgg agcaatatct
     3181 gataagagtt tgatagcacc tgttgcaaaa aaacttgcaa agaaagaaaa tatgtatgca
     3241 atagtagctc cagctataac aggtcaaata gatagtaata ttacatatgg tcagttaaaa
     3301 aatgctataa aatcactggg atttgtagat atgatagaag cttcttgtgg agcagatgca
     3361 gttacagttc atgaaagcaa tgaacttatt gaaagaataa aatcaggaga taattacatg
     3421 acaaattcat gttgtccggg attttttaag tatatagaga agatgttccc agatgaaaaa
     3481 gatagaatat catctacagt gtctcctatg gtagcaacag gaagattagt aaaaaaaata
     3541 gattctgatg ctaaagttgt atttataggt ccatgtacag ctaaaaagag tgaggcaaca
     3601 gagaaaagtt taaaagattc agtagattat gtacttactt ttgaagaact aatggcatta
     3661 tttgaagcat ttaatataga tttatcaaaa tgttcaaatg aagatataaa tgatggttcg
     3721 atttttggaa ggggatttgg agcatctggt ggacttgcca aatcagtaca gaactatatt
     3781 gctgaaaagg ggcttagttt tgattttgaa cctataaaag tatctggtcc aagacaaatt
     3841 aaaaagacaa tgacaatggc taaattggga agattagaag gtaattttat agaaggaatg
     3901 atgtgtgaag aaggttgtat taatggagcc ggtaagtttt tatcaggtgt aaaagccaaa
     3961 aatacttttg atagaaaaaa tggacaatct actaaaaaga gtgttttatc taatgataag
     4021 attaaagaat ttgaagatgt aaatttagaa agataattgt tttggtacag tcattttttt
     4081 attatgccac aaatatgcct aaaatttgcc ctttttatat gtgtatttta cagagttaag
     4141 gttgctatac tataaatatg taataaataa aatttatagg agcttttgga tgaggaaaat
     4201 agtattaaaa gatatcctgt ttttagttac tacattatct ataatagtaa tattattgct
     4261 tgtaacaaat ataactacta atagtaagat aaaatcaaca agtgaatctt atttaaaaag
     4321 tgacaaaaat agtgtgaggc aaaatggtat ccagaggagc acaaagagta gcacaaaaaa
     4381 tactgcattt aagactacta tactaaacac tggaaagtct gattgcatat tggttgagat
     4441 aggtaacaaa gtcataatga ttgatacagg agaagataaa aatggaaaac aaatagtgga
     4501 taggttaaaa gaaaaaggaa taaatacact tgactatttg attttaactc atttagataa
     4561 ggaccatata ggtggagtag atagtgtgtt atctagtgtt aaagtaaaga atattataca
     4621 agcaaattat aagaaagata gtaagcaata tgatgaatat attgactcac ttaaaaaagc
     4681 tgatatagaa ccagtgctac taaaagataa tatgaatatt gtaatcaatg gtgcagaaat
     4741 aaacatacat ccggcttcaa aaagcaagta cgagagttca aatgattatt caataatcac
     4801 taatataagc tatggagcac ataagttttt atttgcagga gatgcagagg aagaaagatt
     4861 gtcagagttt ataaatggaa atactttaaa atatgatttt gttaaaatgc ctcatcatgg
     4921 aagatatgac aaattaactg aaacattttt agagtctatt tcacctcaat atgctgttat
     4981 tacatgttca gagaaaaaag aaccagagga aggtgtttta aaaatattgg aaagattgaa
     5041 tataaaaaca tttttgactt ctaatggaga ggtagttatc aatagtgatg gtaagacatt
     5101 gagtgtgaat caataatttt tttaaaaata tctaaaaaaa tttaaaaaaa tcattagcaa
     5161 atattacccc ttaaacatag tatattttag gagctgaaac tccacataca aaatgactag
     5221 gaggtaacat attatgttgg atagattttt tggtgaaggc ggatttggtg gatgtgcttg
     5281 gtggataata atattattct tcttattctt agcattccaa gattgttggg ctgaaatgga
     5341 cttatgtgca tggatacctt tcttaatatt attattaata ctttgtgaat gtggtgacgt
     5401 ttttgatgga ggatgtggat gctaaaaaaa tcaatttaga tgatttaata aagcttatgg
     5461 tgggtataaa taaaatacca aaccaatcac aatctagtgt ttgaaggtag tcggaaatcc
     5521 cccgctacct tctttttttt attgttaaaa tttatttttg aattatttca tttacatata
     5581 ataattttta cgataaagtc tgatataatt aaaaagaaaa taaaatttat ttataggaga
     5641 tgtaaaaatg gctatagtgg ataagatttt caatgagata aaacttatgg ttgcaagact
     5701 tttattggga aaaaaataca gtgaatatga agaaaaagat ttagtgtata atacagaaga
     5761 agaagttata cttataactt taaaaagatt agtttttcag ggaaatgtaa acgaagcaga
     5821 agaaatttta tttgataaag ctaagagtgt aaatagtgaa aatatgcagt atatagcaat
     5881 agaattttat actatgctta tggaaaagac tgatgaagaa ttagaagcta tgaatttttc
     5941 taaacaagaa gtttatcaag gtatagaaga tattagaaaa gtattgaatt tagaataaaa
     6001 gatatatgaa aatattgaaa taaattaatg ct
//
LOCUS       DAYATN010000187         5831 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.191, whole genome shotgun sequence.
ACCESSION   DAYATN010000187 DAYATN010000000
VERSION     DAYATN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.191"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            13..1368
                     /locus_tag="ACGI8A_003464"
     CDS             13..1368
                     /locus_tag="ACGI8A_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0181428.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(1486..2154)
                     /locus_tag="ACGI8A_003465"
     CDS             complement(1486..2154)
                     /locus_tag="ACGI8A_003465"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HFH0181429.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREELVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(2221..2649)
                     /locus_tag="ACGI8A_003466"
     CDS             complement(2221..2649)
                     /locus_tag="ACGI8A_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFH0181430.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            2801..3349
                     /locus_tag="ACGI8A_003467"
     CDS             2801..3349
                     /locus_tag="ACGI8A_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HFH0181431.1"
                     /translation="MYTNFNIDFNNFNKKENAIKFMLMGVLLIILGLLCLTFKTLGIK
                     LISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVLIIACILLFVFPQKIQ
                     SIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGICLILSPLFLSRFIVSI
                     MSFFIILFGLVLFFSGNTARKY"
     gene            3468..4403
                     /locus_tag="ACGI8A_003468"
     CDS             3468..4403
                     /locus_tag="ACGI8A_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFH0181432.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNKIDDTLKARNSYQGCKFV"
     gene            complement(4674..5618)
                     /locus_tag="ACGI8A_003469"
     CDS             complement(4674..5618)
                     /locus_tag="ACGI8A_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFH0181433.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
BASE COUNT         2028 a          864 c          740 g         2199 t
ORIGIN      
        1 gagtgatagt aaatggatac aaaaatatct ataaacgaaa acatgagtct aggtaaaaga
       61 ttttttaaat acctagcacc atctgtagtt gcaatgtggg ttttctcact atatacaatg
      121 gttgatggaa tatttgtaag taaaggagtt ggagaattag ctttagctgc tgtcaatata
      181 tcaatgcctt ttattaattt tatatttgca gtttctctat tattttcaac tggtgcatct
      241 accataattg caatctatct ggggaaaaaa gatattaaat ctgctaacga agtcttttca
      301 tttaacttag tatcaataat tatattatca ataattatat tagctataac tttttttaat
      361 ttagatagat tagcactatt tttaggagct acagaatcta caattggtat ggttaaagat
      421 tatttaggaa taataatatt ttttaacgga ttctttatag tgtcttattc tttagaagtt
      481 ataataaaaa ctgatggctt tccaatatta gctactgttg gtgtaataat atctgctctt
      541 acaaacatca tacttgatta cttgtttgta attgaatttg gatggggtgt aaaaggtgct
      601 ggtattgcaa ctggtttgtc tcaagtcttt tctactattt tcttcttaat acatttttta
      661 agaaaaaact ctactttgaa tttttcaaaa tttagaattg attttaagac acttagaaaa
      721 attgtattta taggttttcc tgattcaact actgaattaa gttgtggtat agttgtttta
      781 ctttttaact taagtcttac taaatatatt ggagaaaatg ctcttattta ttatagtgtc
      841 ataaattata taaatacatt agtacttatg actatgatgg gtataactca aggtatgcaa
      901 ccacttacta gcttttatta tggtgcagga aatatagata atgtaaaaaa attattaaaa
      961 atggggataa aagccacaat aatagcatca gttgctgtat ttgcaatttg tatggctttt
     1021 tcaggaccaa tagtctcttt atttatacat ccagaagaaa caatgctatt caatgaagga
     1081 gttcgtgtat ttaaaatatt ctctatatct ttcttattgg taggaattaa tgtaataatt
     1141 tctggattct ttgtatcagt agaaaaacca tcaatttcta ctgttatatc tcttggtaga
     1201 gggcttgtaa tagttgtttt aagtctaatt tcaatgatac taatatttgg tggacaagga
     1261 atatggatga ctactattgt ttctgagttt atatgtctta ttttatcatt agtttttcta
     1321 aaaaagaatt tttctacatt agactctaat ctaaataagg ttgcttagat gataagagtg
     1381 acaatatatg aaaatatcta aataaaaaat acctattata caatcttgca aatattaaac
     1441 ctttgcaaaa attaggatta tataataggt attttattta tgtaattatt ttatttctaa
     1501 atatccatag ccatctttta ttaaagttcc ttttacttca tattctacag gttttttaaa
     1561 tattggacca tctacgacaa atgtatgata ctctccatgt tctccacatg ggtctgcacc
     1621 agcatttttt atcttctcca caagttctct agttaatttt tttcctaaaa attcctctcc
     1681 taagtgcttt aagtttactt ttgttacaac actacaaaat ccactgtcta taaattcata
     1741 tactaactcc tctctactct cttgccataa tggtaaatct actttcatac caactgcttc
     1801 acatctatca gtatcccatt ttctatgact ttctatatct atatctccat atgcacatat
     1861 agttgctccc atgtttttag cttttattaa agccctctca aagctctctt catatccttc
     1921 tacaccacac tcagcaacta ataaaggtaa tcctatattg ctacttactt gcttaagcat
     1981 attatattct aaattatgtg tccatgactt tccatgttct tcacttatag ttgtcaagag
     2041 tgccacaggc ttatatcctt ttttcaacat tcgatttaat gctaatatac tatccttacc
     2101 accactaaat gacattacaa atttttcatc agccaatttt aacacctctt tcatctaact
     2161 ttgttttatt aacataatta taccaaattt ttgagttgca tagcaaatta tattaaattg
     2221 ttaattcact ataaagaatg tccctaatat taaaagtata acccctatgt atttgtataa
     2281 atgcattgtt tcttgtccca aaattacgct atcaataaaa aatccaacta aaacttggga
     2341 tacaagagtt aatataagtg tatttgatac tcctaaattt ggaattgcct ttactgtaaa
     2401 atatattatt agacctccaa atactcctcc taataacaac ttataattaa aaccatttat
     2461 agatactaga cactttaagt cacctgtaac tattatactt gccaaaaaaa atattgctcc
     2521 tacagttaag cttataaagg tggccactaa tgctgttgta ctttttccta gctctccatt
     2581 tataaaagct tcaagagctg tagaaccacc tgctaataat gcaaatatta ttgcccataa
     2641 ttttgtcatg gacaaacctc cttctatata attctattaa tagaagttta tgatacaacc
     2701 tattaattaa ttactaagta tagtttaatt tttatacact aggctttatt tggatatgaa
     2761 tttctgttat aattgttcta tcgattttgg aggtgatttt atgtacacaa attttaatat
     2821 cgattttaat aactttaata aaaaagaaaa tgccattaaa tttatgttaa tgggagtatt
     2881 actcattata ttaggattat tatgccttac atttaagaca ttaggtataa aattaatttc
     2941 ttggactttt ggtattgcac ttttattctt tgcatattta aatttaaaaa atattaatga
     3001 actaaaaaga tatgctacta aagaagaaat taaaccctct attaatatcc aatgggttct
     3061 aataattgca tgtatattac tttttgtatt tcctcaaaaa atacaaagca tattttcatt
     3121 atttttagga ttttatttaa tctttaatca actagtagca cttgtaaata gtaagaataa
     3181 tccttattct aaatttacaa cttggaatat agttaagatt ctatttggta tatgccttat
     3241 attatctcca ctttttttat ctagatttat agtatcgata atgtcttttt tcataatatt
     3301 atttggacta gttctatttt tctctggaaa tacagcgaga aaatattaat tattttctcg
     3361 ctgtattttt atattactta aaatagaatt tgtttaagtt atctatttgt ggtaatatgt
     3421 tttttgtaca agctcagcgt acaattttat tagaaatagg tgattttttg gataaattta
     3481 aatatgcttt tgataataaa agatatcata cttggaatta ctatcttaga aatacttttg
     3541 gagaaaaagt ttttaaggta tctataaatg caggatttac ttgcccaaac attgatggaa
     3601 gtcttggtta tggcggatgt acctattgta gcaaagaagg ctcaggtgat tttgctggaa
     3661 accctaaaga taatcttatc agtcaatttt ataatataaa agaaatgatg ttaaaaaaat
     3721 ggcctcatgc aaagtatatt ggctactttc aagcctatac aaatacgtat gctcctcttg
     3781 aagttttaaa agaaaaatat gagactatat tagaacttga agatgtcata ggtctttcaa
     3841 tatccacaag acctgactgt ttgccagatg atgttgtaga atacttgtct gaactaaata
     3901 aaagaaccaa tttatgggtt gaacttggac ttcaaacaat tcatgattct acatctaaaa
     3961 ttataaatag aggtcatgat tataaaacat ttgttgaagg tgttgaaaaa ttaaagtcta
     4021 aaaatataaa ggttgtcgta cacattataa atggtcttcc tggtgaagat tataatatga
     4081 tgatggaaac agcaaaggca gttggtaaaa tgggtgtaga cggtataaaa atccatctat
     4141 tgcatgttat aaaagatact cctatggaaa agatgttaca aaatggtatg ttaactctta
     4201 tggaacaaga tgaatatatc aatcttgtct gtgaccaatt agaaatactt cctgaaacta
     4261 tgataatcca tagactcact ggtgatggta agagggatga actagtaggt cctatatgga
     4321 gcctaaaaaa atgggaaatt ttaaataaaa ttgatgatac tttaaaggct agaaattctt
     4381 atcagggctg taagtttgtt taatacaaaa taataactat agaaatggtt atacaaatat
     4441 agtcctttct atagttattt agtatttaca aaacaaaata ttaatttgta catttataca
     4501 atatataata aaaaatagat agtctaaact gtatctactt atattttatt tgtactgtcc
     4561 actttattct ataaaattta taaatagtta ttttttaata ccaaatataa tttggtataa
     4621 tttttgttgg aatctacaat gtataccaat aaatcctttt taagtatata attctaaata
     4681 ctttcagttt tttcaatctg ctttaacttg ttggacttat aaaatattct tatttcatca
     4741 taagaaagat taagtgctac tactgcagat attaacatca acatcactcc aaataacttc
     4801 cctaaggaaa aactttctcc taatatagtg caaccaatta ttactgaacc aactaattct
     4861 actgatgcca caacacctgc aactgacaac tctacacctt tagatatacc tgctgcataa
     4921 aaaatatatg caagtgcagc tggaacaatg cctaacatta gcatacaaga taaaacatct
     4981 aaatccttag cataatttaa gatttcccaa ggtcttgaag aaggaatcat aaatatagct
     5041 ccaaataaga aactgtatac taatattgtt gaacttgaaa attcctttaa agcattttta
     5101 ctaatagttg gcataagtgc atatgctatt gctgataata ctccaagtaa aagcccaaaa
     5161 gcgtttaaac cttgaaaatc caaacgtcca ccagtcactg ccataattgc accaataaaa
     5221 caaagaataa gtgaaaataa tttgcttcta gtaatatttt ctttataaca taattttgaa
     5281 aatattgcta aaaacagtgg tgatgtatat agtaaaactg ctgcaattga tacacccgca
     5341 atgtcaattg ctttaaaata acataaatta aacattgcct gacatataat tcctattatt
     5401 actgaatata taattccttt tttgtttatc tttaagattt gtggtgtcct tatagatgaa
     5461 tatacgccta aaactataaa tccaaaaaac aatcttgtaa atgctacttg ttctgatgtc
     5521 agtccatttc ccattaaaaa ctttccaaat aatccaagtg tagcccataa taaccctgct
     5581 attgctataa atacataccc tttacttttt tctcccattt taaattccct tctattttta
     5641 tttagtcaat ttacactctt ctttttaagc aaaaactatg ccatcttaaa tcaatatata
     5701 tcgactctaa aaccctagtc tttccaataa tactaataaa ttttcaattt ttttatattt
     5761 atttttcgct tactttaagt tgatttagac ttattttaat tgtaaaatga gttatatata
     5821 acttatttaa a
//
LOCUS       DAYATN010000188         5739 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.192, whole genome shotgun sequence.
ACCESSION   DAYATN010000188 DAYATN010000000
VERSION     DAYATN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.192"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(241..762)
                     /locus_tag="ACGI8A_003470"
     CDS             complement(241..762)
                     /locus_tag="ACGI8A_003470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0181434.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHKQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEIRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            1118..1915
                     /locus_tag="ACGI8A_003471"
     CDS             1118..1915
                     /locus_tag="ACGI8A_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HFH0181435.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            2025..2615
                     /locus_tag="ACGI8A_003472"
     CDS             2025..2615
                     /locus_tag="ACGI8A_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFH0181436.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            2681..4378
                     /locus_tag="ACGI8A_003473"
     CDS             2681..4378
                     /locus_tag="ACGI8A_003473"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFH0181437.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            4592..5431
                     /locus_tag="ACGI8A_003474"
     CDS             4592..5431
                     /locus_tag="ACGI8A_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFH0181438.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         2236 a          583 c          955 g         1965 t
ORIGIN      
        1 tagaataaaa tttgttatat aaaatgtaat tttgtctata aaatctcacc tgttttctta
       61 actttaagcg tgaaaaagag gatgtatcat gaatttttta gttcatgatg catcctgctt
      121 tgtatgaaat ccaatatatc tttattattt aaacaatgga aaagagtctt atttatattt
      181 tgagacatct cttttattta attatgaagt tttatacagt tcaataaata ggaattatat
      241 ttagaaactc atgacattat ttcttagata gtgattaaat ttatcatatt ccattttaga
      301 taacttttct gggtaagaca ttacaatcag gttatgccca ttaaattttt tatggtatgg
      361 tggatgaatg tgttcataat tatttgtctt atattttact ctttcataga acgccttgcg
      421 ataaattgaa atctcatcaa ttggaaggtc aatttctaaa ataatagttt ttcctttctt
      481 atttaaaagc tctaacgctc tttgcccata tctattattg cgaatttctg gaaaaatgca
      541 gaaatgttct acatagataa agttatctgt ttcccaacat aaaattatgc cgacaaactg
      601 gtttttatca taaattaaat tgaattgata ttctttattt ttcaaaattt ctgcttgcaa
      661 agaagatttt ctttgtttat gaaatgggaa actaatttta tataattcca tagccttgta
      721 atataattta ttttcactgg ttcttaaccg ttcaaatttc actttactcc tcctaatcac
      781 gaaaattcca atttatttat gagttcattt taccatagtc agtatttgca tacaatgtgt
      841 tttaagttga gtgtcatttc tttcttttat ttatgcttat atctcatagt ctaagagata
      901 attgcttaag acataactaa aaactattat attaagcttt gataatctaa aaacagtata
      961 tttatggact tttctaggtc taaaagtaat cttaataatt ttagaagtta caaaaaagtt
     1021 acataaattt ggaaagctat aatatacata tataatataa ggttaaaact gtaatgaata
     1081 atattaattt aaaatttgaa catggaagga gattattatg gagaaaagag aaaagaggat
     1141 aataatatca tctttattaa gtgtatcaat tttaatgggt ttggcaagta tatattctat
     1201 attaaataaa gaagacataa gtttaaccgt taaaggtcaa gagcaaaaag tatcttcctt
     1261 taagaaaaca gttgaggaac ttttggatga acagggtgta aagtataatt ctgaagataa
     1321 gattaatcca agtttagata cagagttaaa agatgatatg aaaataaaag ttgttaaggt
     1381 aactaaaagt aaaaaagaag agattgaaaa aattccattt gatacaaagc atgtaaatga
     1441 tagtaatttg ttaaaaggaa aatctaaagt ttatcaagaa ggtcaagaag gagaaaaaaa
     1501 actagtctat aatttaactt accatgatgg caagttagtc aaaaaagtct tatcaaaaga
     1561 agtaatatct aaggagccaa ctacaaaaat tataaaatat ggaactaaag aaaaagtact
     1621 aatagcatca agaggagcaa atataagagg aggcaaacat atgaaagtgg ttgccactgc
     1681 atatgcagga gatacaataa catctactgg tacaactcca agatggggtg ttattgcagt
     1741 tgacccacgt gtaataccat atggaacaaa agtatatata cctaaactgg gtatgacttt
     1801 tgtagcagaa gattgtggag gtgcaattaa aggtaataga atagacattt ttatgaacag
     1861 tgaaggaaaa gcttcaaatt ggggaaggaa aagtatagat atatatctac attaaattag
     1921 tgaatagaat aactttactt gaaggacaaa aaagtataaa atatgaatta actaaactgt
     1981 tagattattc ttaaaacata aaaatatatc taagggggga taagatgaaa atagtctgct
     2041 taggagacag tttaacgtat ggatttggag tatcacgaag taattcatgg acaaacattg
     2101 taaataaaga gactcgatta gaaatagtaa ataagggtat aaatggtgat acaacaagtg
     2161 gtatgttagc taggtttaat gaagatgttg taaaaaactc tcctgatata gtttttataa
     2221 tgggtggcac caatgatttt attgctggtg cgggaaatga aattattaat tctaatataa
     2281 tggctatggt gcatcagtca tattcaaaaa atattatacc tataataggt ataccattaa
     2341 aaccagatat tccaaatgtt agggaagatt ggagttgttt tactgatttt aatatagtat
     2401 ctcaaaactt agaatcatat agttattgga taaaaaaatt ttgtatgaca tttaatacta
     2461 atttcgtaga tttttattca gaatataata aaaatatgga actagaagga tataaaaaat
     2521 tatattttga tgggttacat ccaaccaaag agggtcatag aataatggca gacattttta
     2581 ttaattcaat taataagtat atagaagagt tatagattac attaaaatgc ttgtttatgc
     2641 gtcagttgca ataaatacat acagtagaga ggggtttatt atggattata ctaaaactta
     2701 tgaagagtgg ataaagggtt catattttga tgaagataca aagctagagt tggagaatat
     2761 aaaaaataat gaaaaagaaa tagaggatag attctataag gatttagaat ttgggactgc
     2821 gggtcttaga ggaataattg aagcaggaac gaatagaata aataagtaca cagttagaag
     2881 agctactttt ggattggcaa actatatatt agaaaacaca acaaaagaag aaacaagtag
     2941 aggagtagta atagctcatg ataatagaca caagtctaga caattctgta tagaatctgc
     3001 aaatactttg gcagcatgtg gtataaaagc atatatattt gatagtttga gaactactcc
     3061 agaattatct tttgctgtta gaagcttaaa tgcaatcgct ggtatagtca taacagcaag
     3121 ccataatcct ccagaatata atggatataa ggtatactgg gaagatggag cacaagttat
     3181 gccagagatt gcaaatgcaa taacagagaa agtaaatagt atacatgatt atagtacaat
     3241 accaacctta acggaagcta ataaaaattt ggtagttttg ttggacgaat cacaagatac
     3301 taaatttata gaagcagtaa aaagccaaat tataagaaaa gatttagtta aaaatgtagg
     3361 taaaagcttt aagatagttt atacacctct ttgtggaaca gggaatgttc caataagaag
     3421 agcattgaaa gaagttggtt ttgaaaatat aattgtagtt ccagaagaag aaaatccaga
     3481 cccaaatttt gcagggcttg attatccaaa tccagaagag aaaaaagctt taaatagagg
     3541 tatattactc gcaaaagaaa aaggagcaga ccttgtaata gcaactgacc cagattgtga
     3601 tagagtaggt gtagcagtaa aaactactac aggtgagtat gctttactta caggaaatca
     3661 aattggtggt atgctgacac attatataat agaaggatta aaagaaaata ataaattaaa
     3721 agaaaatcct acaataataa aaaccatagt tacatcagaa tttggagcag atatagctaa
     3781 agcaaataat gttgatgtat taaatgttct tacaggtttt aaatttattg gtgaaaaaat
     3841 aaaattattt gaacaaaata aaaatagaag ttatgtattt ggatatgaag aaagttatgg
     3901 ttacttagtt gggactcatg ctagagataa agatggtgta gtttcttcat tgttaatatc
     3961 tgaaatggca gcattttact attcaaaagg tatgagttta tatgatggct taatagaact
     4021 ttataaaaag tatggatttt tcaaagaaca gactatatct ttgactttaa aaggtataga
     4081 aggtgtagaa aagataaaag aaataatatc ttattttaga gaaaatcaaa ttgattatat
     4141 aaatagtatt aaagtagttg ataaaaaaga ttataaaaat ggtatagata atcttcctaa
     4201 atctgatgtt ttaaaatact ttttggaaga tgaatcttgg gtagctataa gaccatctgg
     4261 aacagaacca aaacttaaat tttacatagc tgtaaaagga gctagtgata ttgaagcaga
     4321 taaaaagatt caaggtttga aaaagtatat agatgacatg gtcgaaaaat taaaataaaa
     4381 tattattatt ttataagata attttgtaat aatatttatt tagctgaaaa ttataattta
     4441 ctttcattga gtagaatatg gaaatgttag tagaattgtt attttcagct tttatttgtg
     4501 ttatttttac ttaaataatt atattataga atgaatctga gtataattaa tattgtataa
     4561 tgaaattaat ttgatagaaa aggggatatt tatgtttggt atagataaga aattgattga
     4621 atatataatt gcaaaatcca attacagtga attaagttca aagtcagcag aagtatcttt
     4681 cttcttaatg ttgtccatat ttcctttttt aatatttaca ataagttcta tagcttatat
     4741 accgattctt catttaaata agtatatagc tttgtttaga aatatgatgc cagaaggtgc
     4801 ttttgctgtg ttatcttcaa tcatagtttc tgcaatagac aatagaaacc tgaaattttt
     4861 agcagtcagt tttgtactta ctatgtggac attttcaaga gcagtaaaag ctcttattaa
     4921 aggaatgaat agagcctata aagttaaaga aactaggtct ttttttaaaa ttttatctat
     4981 atcattttta tttacaataa tgcttttggt ccttattttt ttatctatga tatttttagt
     5041 ttatggggaa aaaataggtt attttatttt taatcttgta ggattggatg agatatttat
     5101 aaaaatatgg gatattctaa gatacactgt aggtataata acaataatag tgatatttac
     5161 acttttatat aaatacactc caaataaaaa gttaactatt aaagaatctg cgccaggtgc
     5221 tatttttgct acttttgctt ggtttttagt atcattttta tattcttatt atacaaatta
     5281 ctatgctaac tatgaagtaa tctatggaag tatagctgaa ataattgtac ttatgacctg
     5341 gatgtatttt agtagttggt ctatcgttat tggctatgag gtaaattcaa gattgtattt
     5401 tagaaagata agacatgaaa tgttaaagta aagtatttaa aataaaagag agtatttagt
     5461 attataaatg taattgaatt aataaagaaa tattgacatt taagttaaaa agtctagaat
     5521 ttttctgggc tttttttatt tttagtataa cttgacaaat tttatttaat gggatagttt
     5581 taatttataa tcatctttaa aaagatgact attgataagt aagagataaa attggtaagt
     5641 atcaaaaaat atactaaata aaaattatag tcctaatgag ttaatttctg tattttcaaa
     5701 taatgcagta tttagtcatg tgtttttata tgcgcctgt
//
LOCUS       DAYATN010000189         5571 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.193, whole genome shotgun sequence.
ACCESSION   DAYATN010000189 DAYATN010000000
VERSION     DAYATN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.193"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            152..1333
                     /locus_tag="ACGI8A_003475"
     CDS             152..1333
                     /locus_tag="ACGI8A_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0181439.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1475..2392)
                     /locus_tag="ACGI8A_003476"
     CDS             complement(1475..2392)
                     /locus_tag="ACGI8A_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotA family spore coat protein"
                     /protein_id="HFH0181440.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2645..3439
                     /locus_tag="ACGI8A_003477"
     CDS             2645..3439
                     /locus_tag="ACGI8A_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181441.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3440..5500
                     /locus_tag="ACGI8A_003478"
     CDS             3440..5500
                     /locus_tag="ACGI8A_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HFH0181442.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2132 a          629 c          917 g         1893 t
ORIGIN      
        1 tttaatgttt ttgtgtataa ttataacaat ttaatttata ttaatgcact taaatttgaa
       61 atagaatgct tatagtggta aaattaatta ttagaatatt agaaataata aataaataat
      121 acattgtact taaaaggggg agaaatttaa gatgaacaaa aaatatatta aagcagggaa
      181 tttaattgat ggaaccggaa atagtattat gcaaaataag ggtataatta ttgatggaga
      241 tactatagtt gaaattgaag atataagaga tgatttaaat ggatatgatg tgtacgatta
      301 cttagataaa actgttatgc caggcattat gaattgtcat gttcacttga caatggagcc
      361 agttggaaat ccaaagatat attatgataa tgtatcagat gtagagcttg tagtaaatac
      421 gataaaacaa cttgatgctt atttggattc aggagtaaca tatattagaa gcttaggttg
      481 tcccaaatat gtggatgtac aacttaaaaa tcttatagac aaagggtcta tagatggtcc
      541 tggtatagtt acttcaggtc cagttatatg tatgacagga gggcatggat attattttgg
      601 tattgaaagt gatggagtag atgaatgtag aaaatcagcc agaacagttt taaaaaatgg
      661 agtagattgt gtaaagataa tggcaactgg aggtgtaaca actgatggag ttgagccagg
      721 gtcaccacaa ttaactttgg aggaaatgaa ggctgctgta gatgaagcta taaaagcagg
      781 taaaataact gccacacatg cacaaggaag aactggtata aaaaatgcag tgcttgcagg
      841 aattacttct atagaacatg gtgtttattt agatgatgaa ataattgatt tgatgcttga
      901 aagaggaact tacttagttc caactgtggc tgcaccatat tttattctaa atgcaggaaa
      961 agaaagtgga ataactgaag ctactcttag aaagtgtgag attgttgcca ttccacacaa
     1021 agagtcattc ctaaaagcat ataaaaaagg aattaaaatt gcagttggtt ctgatgcagg
     1081 aacttcttat aatgaacatg gaaaaacata ttatgaaatg aaactaatgt cagattatgg
     1141 tatggataat atggatataa tagttgctgc tacaaaaaca gcatctgaac ttttaagaat
     1201 tgataaaaat tatggaactc tagaaaaagg aaagaaagca gatgttatag ttgtaaatgg
     1261 aaatccagta gagaatatag acgtacttgc agatgtgtta gcagtattta aacttggtaa
     1321 aaaagttaga taaatagtat aaaaataaaa aagagtgtct aaagaaattt ttaaataatt
     1381 atagacactc ttttttatta gtaataatta aatcttttat actttagaaa atagattcta
     1441 ttttatattt agatataact ctttaaaatc attcttatgc aatataatct atagaatcta
     1501 cacatacaaa gtatattctt gaatcattag aatttccaag tactataaca tcatttttct
     1561 tacctagaac ttcaacacca gttagaacca aactaccagc tactacagta acttgattat
     1621 ttatatttga catataaaga ttatctagga tacctttatt acaacagcaa tcatcacaat
     1681 cgcagttgca attacattct ttgtaatgtt ttttatcaag atagtaacct aaagatgcta
     1741 tgaaatcttc ataatctgca ggtgtaatat caaatgcaac agctttaaga ttacacaatg
     1801 atgctaaatc tacgtttgct aatggagtag atgcagcaag tataaaatca attatagcag
     1861 caagtattgc atctaatata gccccaaggt ctatcgtagg aggaataact gcatcaataa
     1921 gtgcaattaa ttcagctact cctggtatag ttcctatagt attaattaat ccctcaagag
     1981 ttaaagggac tggaatagga taagatactc taccagctat atctattaaa tcacagttac
     2041 aagcagaaaa tctttcaaaa gtaccatcaa gtccagataa attatctttt ggaggagctg
     2101 aaagatctat acctaccaaa ttagcaccta ctataaagaa atctgagata aaagcaaatt
     2161 gattaaagtt tacaaaaggt cttagagcat catatcttag aagttctaat gcttctctca
     2221 tacttttttt acaacaacat tgttttattt taggttcttt atatttattt ggataattat
     2281 agtatctatc agctacttga taattttcat agtatctttc atttgtattt ggataatctt
     2341 cctcatattc ttgtgtaaat ctaaagtcct ctctacattt attattttcc actaaataca
     2401 ctccttttat attttaattt aaatgtatag ataattaagc ttatctatat agtaatatta
     2461 tatttataat gtaaaaaaat gttacaaaag atattataaa cgaataaaaa ataataattt
     2521 gtcagataaa cttgtaaatt tttatgaata atccattcta aaacaaaaaa ctgtgtataa
     2581 aaaaggttta tatgttatat ttatataaat ataacatatg aacgagaaag gagtggtttg
     2641 ataaatgatt gaggtagata aattgtgtaa gtcttttaca agggttgtaa aagatgataa
     2701 gaataaaagt tcaataagaa agctaaagaa agtaaaaact aaaaaagaag aattttttgc
     2761 tgtaaataat gtttcattta aagttatgga aggtgaaatt gttggaatat taggtccaaa
     2821 tggtgcagga aagactacat tacttagaat gttaggtggc atattaacac caacatcagg
     2881 aagtataaat atttctggat atgattattc aatagataga aattcagcaa aaaaagaaat
     2941 aggttatctc tctggaaata caaaactata tggaaggctt tctccgagag aacttctaac
     3001 aacttttgca agtctatatg agatgtcaaa agaagatata gaagaatcaa ttgaaaatat
     3061 tgtaaaaata atggatatga gtgaatttat agataataga attgaaaatc tatcaactgg
     3121 tcagactcaa agaacatcta tagctcgttg tttaatacat tctccaaaag tatatatttt
     3181 tgatgagcca acactaggtc tggatgtttt aagtagtgca tctattatag attttatgaa
     3241 gaatgaaaaa ctaaggggaa aaactgttct ttactcaact cattatatgg aagaagcaga
     3301 aactttatgt gacaagattt ttatgattca caatgggaaa ataattgcat caggaacacc
     3361 acagagttta aaagaggaat ctggagtaaa taatttgaga gatgtattca taaaacttgc
     3421 cagaaaggag gacatttaaa tgagaagtaa gattgtaaaa tacatattta aaaaagaaat
     3481 gttagacata ttgagagata aaaagacttt atttatgatg attgttgtgc cacttctttt
     3541 atatccttta cttatgttat taatgattca agtaatgaat atgtctgcaa gtagtatgaa
     3601 tagtaaggac attaatatag cttttaataa taaacctaat aaggtgttag tatccatgat
     3661 tgaagatgat aatttagaaa aagatactaa aaatacttca aatgataaag gcaaaataag
     3721 agtgttagat gtagataact ataaaaagtc attagaagaa aataaaattg atgcttatat
     3781 aaagatggaa gaaaaaaaat catctataaa ttatcaaata tatataaatt cttctcaaga
     3841 aaattcttct aatgcactaa aaagaattga aactgttttg gataaatata aaagatttac
     3901 catagaaaaa actttatctg aaaaaggatt agatgtacaa gctactttag aaccaataac
     3961 atatagcact gtagatgttg caaagacgga agaagtggca ggatactttt taggtcagat
     4021 acttccattt atattaataa taggagtttt actaggttca atatatccag caatagatgt
     4081 tatggctgga gaaaaggaaa gaggtacatt agaaacatta tttacactgc caatatcaaa
     4141 cttagaactg gttatgggaa aatatatggc agtttctctt agtgcaatag taacagcaat
     4201 tttaaacatt ttatctatgg gactgactat ggcatttatt ttagttagtg gaggtattag
     4261 tagccaattt ggctttggaa actttaatta tggagactta gtcttaccaa ttataactac
     4321 tttaatttgc atatgtcttt tttcaatggt agtttcagca ataagtatgt gtgtatgttc
     4381 attagcaaaa agttttaaag atgctcaaaa ctacataaca cctcttatgt taattattat
     4441 gataccatca tatgtttcta tgataccaaa tatagtatta gatagagtga cagctgtaat
     4501 acctgtagta aatatatctt tacttataaa aagtgtatta tcttttaaaa gcgatttaaa
     4561 tttaatagcc ttagttttag catcaaatgt agcctttgtt atattggcaa tcatactact
     4621 gtctaagatg ttcaattctg aggaaatact ttttggtaat agtaagagtt tttctttctt
     4681 agagaaaaga agtaatataa aaaaagggtc tataccaact gtaagtgatg gtgttatttt
     4741 atatgcagta ggattagttc tattgattta tgttggttct ttagttcaaa ttaaatttgg
     4801 tatggcaggt attataatga cacaattcat gataatttct ttaccattat tatttgcata
     4861 ctatattaaa gctgatttta aagaggtatt tagcttgaca gttcctaaat taaagcatgt
     4921 gtttgggagc attgttcttt ggattggtgg atacatactt attatgatta taacacagct
     4981 aattttattc ttatttcctc aaaatatgga agttgctgaa agtttaaata aggctctttt
     5041 tatcaaagat agtgttttac tgaatttact agcaatagct ttattaccag ctatatgtga
     5101 agaaatgttt tttaggggat ttatattttc ttcatttagt aaatcaaaag ataaaaataa
     5161 gtctatcaaa ctcgctataa tatgtagtgg ggtactattt ggtattatgc atatggattt
     5221 tattagaata attccaacat caatattagg tattatattt gcatacagtg tgtataaatc
     5281 tggttcaata tttgtatcaa tgttattgca ttttttaaat aatagtgtag cagttgtgtt
     5341 aaatcattat acaactggaa atataaccaa cttatataaa tttgttgaag ttgatttttc
     5401 gaacttaaat gtgcttaaat ttgccttaat tcttctaata agtcttatat taattatgtt
     5461 aggattaaga gttttagata gaaagtcttt aagaaattaa gtataaagtt attatataga
     5521 ttaaaatatt aataagcact aaagtatatg gatatatctt gtatataaaa a
//
LOCUS       DAYATN010000190         5524 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.194, whole genome shotgun sequence.
ACCESSION   DAYATN010000190 DAYATN010000000
VERSION     DAYATN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.194"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            241..1077
                     /locus_tag="ACGI8A_003479"
     CDS             241..1077
                     /locus_tag="ACGI8A_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFH0181443.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1430..3007
                     /locus_tag="ACGI8A_003480"
     CDS             1430..3007
                     /locus_tag="ACGI8A_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0181444.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3000..4184
                     /locus_tag="ACGI8A_003481"
     CDS             3000..4184
                     /locus_tag="ACGI8A_003481"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFH0181445.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            4202..4801
                     /locus_tag="ACGI8A_003482"
     CDS             4202..4801
                     /locus_tag="ACGI8A_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFH0181446.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4825..5295
                     /locus_tag="ACGI8A_003483"
     CDS             4825..5295
                     /locus_tag="ACGI8A_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFH0181447.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2087 a          569 c         1041 g         1827 t
ORIGIN      
        1 ataaaatgtc gattgatttt aaataattaa atatacataa gaaataatta aatatatttc
       61 aatgaaaata tgataaaata tgacaaatta attgatgaca ataaaaaata ttttgttatt
      121 gtagtattat agggtaaatt gcaaaatata tacgaaagta aaattttaaa tattgacatt
      181 aaattaaata tactatacta tgtaagtgtg atgagagaat ttttttagag gggatttata
      241 gtgaatataa aaaagatatt taataacaat gtagtagtaa gctccctaga agatggcaca
      301 gaaataattg tcactggtgc tggagtagga tttaagaaaa aagtaggcga tttgatagat
      361 gaaaatttga tttctaagaa atattttgtt caagacgatc aaagggacaa atacaatcaa
      421 atacttaata agacctctat tgaatacttt aaaatttcag aagaaattat agaaaaggct
      481 aatgaagttt taaatactca agtgaatgat tcaataatat tagccttaac tagtcacata
      541 gaatttgctg tccaaagaga aaaacaaggt ataaaattac caaacttaat attgaatgag
      601 acaaaacaac tttataggga agaatttgaa tttggattat gggcaattga tgaaattgaa
      661 aaaaggatag gaataaaatt gcctgaagat gaagcaggat atattgctat tcacataatc
      721 aatggtttgg aaaattctca aaaagatgaa ggtattaata tactagagtt ttcaaaacaa
      781 gttattcaga taattgaaga agtacatgga tttaaattgg atgtaaattc attaaattat
      841 actagactta taactcactt aaagtttttt gttcaaagga ttctccgaaa agaaacttac
      901 aatgatagtg atattgaaga tatgtataag ttagtgaata aaaattacaa taaacctaaa
      961 gtgtgtactg aagaaattgc agcattggta ttagacagat ttgaatataa gattagtaaa
     1021 gaagaagaat tatatttaat gattcacata aataaaataa caaacagtgg ttgttaggat
     1081 tgtaactgat aaagcaggca aaacctaagt cattgagctt aactattaga ttaagttttt
     1141 tgatttaggt ttttttatta gatacaatca aaatacagac agtgactttt aggattgtaa
     1201 ctggttaagc aggcaaaacc taaatcattg aatttaacta ttaggttaag ttttttgatt
     1261 taggtttttt tattagatac aatcaaatac agacagtggt tgttaggatt gtaactggtt
     1321 gagcaggcaa aacctaaatc attaaactta attattaaat taagtttttt gatttaggtt
     1381 tttctataaa tttgattatg tttttagata aaaataggag gggaaaatta tgaaaaaaaa
     1441 taagttaggt ttatggaact tcttccaaat gttaggaaaa acgtttatgt tcccaatagc
     1501 actacttagt gtttctggga tgatgctagg acttggggca ggttttacag accctaagat
     1561 gattgaaatg gttccatttt tagggaatga atatgttaac ttagttttaa acttcatgtt
     1621 aacaatagga ttatttgcat ttaacaactt gggagcatta tttgcaatgg caattccact
     1681 aggattatta aaaaaagaaa aagagtttgg tgctttttca ggattagtag gatttatagc
     1741 aatgcatata ggtacaaatt tttatttaac aagagctgat ttgttagtac cagcagaaca
     1801 gatgtcaact aatggtcaag ctatgattat gggtatacaa acatataata caagtgtttt
     1861 aggtggtatt gtagcaggat taattgttta tgcaatatat gataaagtta gtaatttaaa
     1921 gataccagaa tcattaggat tctacagtgg accaagatta gttccaattg ttagtttaat
     1981 agttatgagt atagttggat tattaatacc tattgtttgg ccaccatttt tcaatgcatt
     2041 ccaaagttta ggaagatgga tttcttcagc aggaccatta ggatattttt catatgcagt
     2101 ggcagaaaga gttacaattc catttggact aaatcatctt gtaacatctg tattccgttt
     2161 tacaccaata ggtggaactg ctgttattga tggagaaacg tactttggta cattgaacat
     2221 gtttatggca tatgtagaaa ataatcagat tatacctctt gatttagctg gtaaaatgga
     2281 acaaggtaaa ttgatgattc aatatggatt agctggtgct gcattggcta tctaccgtac
     2341 agcaaaacca gaaaatagaa aggcacttaa aggactttta atatcaggag tgttgacagt
     2401 tattataggt ggtataagtg aaccaataga gtttctattc ttattcatat caccagcatt
     2461 atttgctttc catacattta tgaatggtct agcaaacatg gtacttccat acttaggcgt
     2521 gttgatggga ttcacaggag atttaattgc atttataagt tttggtgtgt taagaggaac
     2581 agctacagga tggccaatag cagtattagt agcagtttta tattttgcaa tatactattt
     2641 tgtatttaag tgggcaatta ttaagtttaa tataaagacc ccaggacgtg aggataaaga
     2701 aataaaagta tctgaaggaa atggttcaaa tttcaaaaat ttgtctacat ataaaggaca
     2761 acaaatgata tctgctttag gtggtcaatc taatatatta tcactagata attgtgtaac
     2821 tagattacgt ttaaaattac aagatgtatc attaataaat gaggatgcaa ttaaagaggc
     2881 tggtggtata gcagttgtta aattggatga gcatacaata caagtaatta taggaaccca
     2941 agtgtattct ttaagaaagc agatggataa agctatggaa ggtcatttgg aggcatgtaa
     3001 tgattaattt tgataaaaaa gtagaccgat tgggtacata ttgtacccag tgggactatg
     3061 tagaagatag atttggtaag aaggatttgc ttccttttac aatttcagat atggatttag
     3121 agtttccatt tgaaataaca gaggtgttgc aggaaaggtt aaatcataga gttttgggat
     3181 acagtagatg gaaacatgat gattttagaa attcagttac gaattggtat attaaaagat
     3241 ttaattgtag cgttgatagt aactggatat attatagtcc aagtgtaatg tattcaatta
     3301 gcaaacttat tgagatgtta agtaatgaag gagatggaat ccttataaat actcctgcat
     3361 acaatgcttt ttatgatgtg ataataaata acaatcgtaa tattataaag tcacatcttg
     3421 taaataggaa tggattctat tcaattgatt ttaatgattt tgagaaaaag tgtaaaaaat
     3481 ctaagatatt tattttatgt aatcctcaca atccaactgg tagagtatgg agtgagttag
     3541 aacttgttaa gatgatagga atttgtaaga ataatgatgt caagataata tcagatgata
     3601 tacacatgga tattgtatat gacaatagaa tgacacctat cttaaatgtt gcaaaggact
     3661 atttagattc tgtattcatt tgtacatcag cgtctaaatc tttcaatata ccagcattga
     3721 ctgggtcata tgtgattata ccaaatgaag gtattagaaa taaatttgaa aatatcacta
     3781 gataccgtga ttttgtaaat tcgccagcaa ttctagcaat attagccaca atggtctgtt
     3841 atgatgactg cgaatattgg ttagatgaac tattaaaata ccttaaagag aatcttaaat
     3901 atacgattga ttatataaaa gataatttag aaccattgaa attggtcatg ccagaaggct
     3961 gttactttgc ttggatagat ttttccgaac ttaaaataag tggtgagaaa ttccaaaaat
     4021 tattgatgga tattggagaa gttgctatta tgtcggggtc tgtatatggt gaagaaggtg
     4081 agttttattt aaggttaaat gtggcatgtt caagagaaaa acttgaagat ggtttaaata
     4141 ggattaaaaa aactatggat tatattgaaa aaagaatttt atagtaggaa gggagatagt
     4201 tatgggatat ttaaaaacaa tggttgatgt tatgactact gaaatgaatg ccataagatg
     4261 tttaatagat gaagcaggaa tagaatatga aagtatagtt agtgaaattg caaattgtga
     4321 aggcaaagta atttttatgg gtgtaggaaa atctgcacat ataggtaaga aattggcagc
     4381 tacatttgct agtactggta cacctagctt ttttgtacat gcaacagagg cagtgcatgg
     4441 agatttagga atgattgaaa gtaaggatat tacaatttta atatcaaata gtggaaattc
     4501 tatggaagtg gtaaactgta taaaatatat taaagcaata ggttctaaga caattgcatt
     4561 tacttcaaac agaaattcag tactggcaaa agagtgtgat tatgctttaa tatatcctgc
     4621 aaaggatgaa gcagaccatt taaatctagc accaacaaca tcatctacaa ttactttggt
     4681 tttaggagat tctattgcat gtgcattatc taagagtagt aattttggtt cttcagattt
     4741 ttataagtac catccaggag gaagtttagg tgagaagtta aaaacagcta acaatggata
     4801 aaagcaaaaa ggagagtagt ttttatgttc aaaatgttta aaaaaaaatc aataaaatca
     4861 cccatatcag gaaaagtaat agagttatct aaaatacaag atggtgtatt ttctcaaaaa
     4921 attatgggag agggtgtagc tatagattct acaggagaca ttgtatatgc tccaatcaat
     4981 ggaaagatag tagtagtagc agagactaaa catgcatttg taatagaatt agaaaatgga
     5041 atggaacttc ttattcatgt tggtttagat actgtaaatt taaaaggcga aggttttgag
     5101 gcattggtga gtatgggaga acaagtgaag gaaggaacac caatgttgaa aattaaccgt
     5161 cctttgattg aatcaaaggg tatatcactt attactccag ttacaataac taatcattca
     5221 gagtacaata tgaatacatg taatgtaggc aatagtgttg aaggtgggaa agatactgta
     5281 attgagttta aataaagaac taaaatacaa ggttaatatt tataaatacc tataaactta
     5341 tgttttataa gattgattgt atagatttgt tgtataaaaa agaactcttt aattataaat
     5401 taaggagttc ttttttattt tgtaaatatt gtttagattt atatagaaaa acacttagta
     5461 ataaatagaa taaataagta tttatatagc taaaaatcta aatagttttg tttaaatata
     5521 tttt
//
LOCUS       DAYATN010000191         5411 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.195, whole genome shotgun sequence.
ACCESSION   DAYATN010000191 DAYATN010000000
VERSION     DAYATN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.195"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(138..746)
                     /locus_tag="ACGI8A_003484"
     CDS             complement(138..746)
                     /locus_tag="ACGI8A_003484"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFH0181448.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(1006..1551)
                     /locus_tag="ACGI8A_003485"
     CDS             complement(1006..1551)
                     /locus_tag="ACGI8A_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HFH0181449.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(1669..3492)
                     /locus_tag="ACGI8A_003486"
     CDS             complement(1669..3492)
                     /locus_tag="ACGI8A_003486"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181450.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRILNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSILLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(3505..5280)
                     /locus_tag="ACGI8A_003487"
     CDS             complement(3505..5280)
                     /locus_tag="ACGI8A_003487"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181451.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1819 a          944 c          467 g         2181 t
ORIGIN      
        1 acaactaaca ctataattct agtgattaat tttatttcaa aaaatataat aacactacta
       61 atattttaaa tatcaatagt gtcataaaaa cttttcagat taattaaatc tttctatata
      121 ttttataata atctaaatca tgctaaatat ttagacagaa aacttttttc tttaatagta
      181 aatacttttg tttgaatatc agaataatta tttgaatatc tatctataaa accatgattt
      241 gcatctaaaa tctcaatttg gatattttgt ttcctagaaa gttggtaaga aatagaatca
      301 acatcaaatg aatcgtgctt tgcaaatact aataaggtag gacattttgg aattacatcc
      361 atgtaatctc taatccgaga gccataacaa caaattatgc catcacaaaa agaatcttca
      421 ctgcatctcc atgcaatagt tgctcccacg ctatacccag caacaaatac atatttatac
      481 tctgatttta aatcatttat aagcttttca acttttttat aaatatcaaa accaacatta
      541 tttataaaaa aatcatatgc ctcttggact tttgaatatg gaaaaactct tttttctccc
      601 agaagttcag gacaaaatac atcatatcca tatttataat acttttgaca aacatcttct
      661 ataaagctat taatcccata gatttcatgc aaaacaacaa tagctacatc acttttatgt
      721 atatacttta tcagattatc caccaccttt acaaaacagc aaatttttta tacttagttt
      781 aatatattct tctttacatg tcaatttact tttctaaaat caaatttatt tttggattaa
      841 tcatttattt ttaaaagttg tgcattatta ctattaaatt attctctatt attaaaaata
      901 taatcgttag aaataaaaaa taaattacaa ataattaaaa aatatctcac tcataacttt
      961 aaaaattata agtgagatac tcctaaaatc tactttatat taaatttatt ttccaaaata
     1021 tctatttaat aatcctaaaa atgctttacc atgtctagct tcatctttac acatttcatg
     1081 tactgtatca tgtatagcat ctaatcctaa ttcttttgct ttcttagcta attttaattt
     1141 tccatcagtt gcaccatact cagcatcaac tctaactctt aagttttctt ttgtatctgc
     1201 aactacaact tctccaagaa gttctgcaaa tttagcagca tgttcagctt cttcaagagc
     1261 tattctctta taagcttctc ctacttctgg ataaccttct ctatcagctt gtctactcat
     1321 tgctaaatac attcctactt ctgtacactc accaacaaag ttagctctta atccttcgat
     1381 tatttcttca tctacacctt gagctactcc tattctgtgc tcatcagccc aatttttttc
     1441 gcctttcatt tcttcaaatt tatcagctcc aactttacat actggacatt gtgctggtgc
     1501 agcatctcct tcatgtatat atccacatac tgtacaaaca aatttcttca taattacttt
     1561 cctcctaaat tttatatatc aaattaaatt ttttatttat taagttagtc tatatatacc
     1621 cgcaccatat acctttaaac aattttttta aaagtttttt ctaaatactt attttccctg
     1681 tgtcaataat atgtttctat aataatgtcc ctttaatttt atcaattctt tatgatttcc
     1741 tgattccaca attctaccat tttctaaaac tattatatta tcacaatttg atatggtact
     1801 taatctatga gctataatta tacttgtctt tccataagtt aaattattta ttgcttcttg
     1861 aattttagct tctgttgcta tatctatgtt acttgtagcc tcatccaaaa taagtattgg
     1921 agcatttttt aatactgctc tagcaatact tattaattgt ttttgacctt cacttaaatc
     1981 ttcaccccta tccgataata cagtactata tccttcacgc attttaacta taaatttatg
     2041 tgcatttgct attttactag catatataac ttcttcatca gtagcctcca gatttccata
     2101 ccttatattt tccataactg tatcattgaa tatataacct tcttgtggaa ctactccaaa
     2161 acaagttctc aaatagtttc tgttaataga gtatatatcc ttaccatcta aaagtatact
     2221 tccttcatct atatgataaa aatttgtaat tagatttata atagttgttt ttccacttcc
     2281 cgtctctcct atgatggcat ttgatgtgcc agctttgatt tttaggttta tattatctaa
     2341 aattttcttt tttgaatcat aagaaaaact tacatcttta agctcaattt ctccctttac
     2401 aaaatgagag tttaaattac catctttatt atcgttaatc tcacttttaa acatgttatg
     2461 cacttcttgc tcttcttgtt catctataat ttcaaaaact ctttcacatc cagctatggc
     2521 tgaaagtaat gtattaaata gatttgctaa ttcatttaaa ggtcttgtaa attgtcttac
     2581 ataacttaaa aaactagcta taacccctat ggttataata tctttagatg caagaatgcc
     2641 tcctataata catatagttg caaatcctat attactaatc acattcataa taggcattaa
     2701 aagagaagtg taaatttgcg atttaaggga aatatttaga attctatcat tgacttcttt
     2761 aaaatcattt acagattttc tttcataatt aaaagatttt actacattta tgtttgatat
     2821 catttcttca atatgtgcat ttagttttcc caactcaact tgttgttgct taaaaaaaac
     2881 tctagttctc tttgcaataa aattactcac aaacattact atcggtatta atattacact
     2941 gcaaaaggtt aaaattgggc ttaatacaaa catcataata aaagtcatca atatgtttaa
     3001 gattcctgta attaactgta ctaatgaatt tgatattcct gtactgatat tatcaacatc
     3061 atttgtcact ctactcataa tatctccagt agaactttta tcaaaaaagg ttattggtag
     3121 atttttaaat tttaaaaata aattacttct catttcttta actatacttt gagataatct
     3181 agctgtacat atccccttag acagtttaac tgaaaactca attatataaa atgaaagtaa
     3241 aataattatt ccttttaaga aaatggattt tgaataagta tcgatatcta ttatgtcaac
     3301 tacctttcca atagtatatg gtattacagt tgatataatt gaatcaatta ttactagcaa
     3361 aaaaataaat ctagtaatct tcttatcttt tttaaagtac ataaaaattc ttttaaacgt
     3421 ttttttaaaa tctttaggct tttctttttt ctttaatcta ctgaccttct gatttccagt
     3481 acttaaattc aatatacttc ctccttaatt tacatcttca cttactaaat tctgcaacgc
     3541 aaaaattttt ttataaattg gagaactaac cagtaaatct tcatgtgtag caaatccaac
     3601 cacctcaccc tcatccaaga ctaaaatctt atcacagtca attatagaac taatcttttg
     3661 tgaaatagtt attacagtac atttcttact ttccttttct cttaaattac ttatatactc
     3721 ttttatattc tttcttataa ttgtctctgt attaatatcc attgcactaa aacaatcatc
     3781 aagaattaat atttgtggtt ttttggctat agctctagct atagtaattc tttgtctctg
     3841 tcctcctgat aaatttttac ccctttggaa taaaacttca tcataagtat tttcgagttt
     3901 tgttataaat tgatttgcat ttgctatttc acatgatttt acaacatcct ctttgcatat
     3961 atctctattt cccataacta cattctccaa tatacttttt ccaaacacac tactattttg
     4021 aaatactaca cctattcttg cccttaaact ttcttcagaa atactgtata tactacgtcc
     4081 tccaatctta atatcacctt tatcaattat atataatttt tgaattaaat ttactaaaga
     4141 agtctttcct gaaccagttt gaccaatcac accaatattt tgaccttctt ctattttaaa
     4201 ggatacgttt tttaatattt tttcatctgt atttttgtta tacgaaaaat ctacattttc
     4261 aaaaattata tcacagttta caaactttaa atctttttct ccaacatcta aactttcttc
     4321 tctctcaatt acttcttcat tctcatcaac caaaacctca cttattctct cataagatgc
     4381 ttttgcccta acaaaaacat taaaaacatg tgaaatcatt ataagagatg ttaatattct
     4441 agtcatatag tttacataag caactataat cccaatttta atttctcctg aataaattaa
     4501 atgccttcca attagtaata ttattattat ggaaacatta atgattattg taattgtcgg
     4561 tctaaaaaat gacatcatag ttgtagcttt tctactattt ttaaaaagtt catcatttaa
     4621 ctcttcaaag ctttggttct cataatcgta tctacaaaat agtttaacta cttttattcc
     4681 acttaaaaac tgtcttaatt ttgaatttac tttatctgta ctttcttgta catttttaaa
     4741 taatctatac cctatcttca tattaagcaa tattataata aatataatta aaactgcaat
     4801 tagaataatt attgacatct ttaaatttaa ttttatagct ataattaaac ttcctataca
     4861 cattattggt gactttataa aaactctcat cattccatta acgaattgtt gcatttgatt
     4921 gatatcatta gtaattcttg ttattaatga agctctacca aatttatcta tttgttttaa
     4981 tgagtatgta tttatctttt caaatacatc ctgtcttaaa tcataagaaa aatgctgaga
     5041 aactcttgcc gagattacac ttcttattat tgcaaatatt gcacctaaaa aagttatacc
     5101 tatcattata gctcctgttg atagtatgta gtctaagttc ttatttgaaa cccctatatc
     5161 tattattttt gccattatag ttggttgagc taaatcacaa aaagcttcca atgttaaaaa
     5221 aaatactgct gttagaaata atactatata tttttttatg tattttttaa aatacctcat
     5281 tcaatcatcc cttcatagaa ttttctcatt accaaaagta tatcataatg gctaatttaa
     5341 ttgattatat caattataaa tatttgattt attattaatt tttataccat atattttata
     5401 aattatttgt t
//
LOCUS       DAYATN010000192         5362 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.196, whole genome shotgun sequence.
ACCESSION   DAYATN010000192 DAYATN010000000
VERSION     DAYATN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.196"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            237..1700
                     /locus_tag="ACGI8A_003488"
     CDS             237..1700
                     /locus_tag="ACGI8A_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFH0181452.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1756..2892
                     /locus_tag="ACGI8A_003489"
     CDS             1756..2892
                     /locus_tag="ACGI8A_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0181453.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3128..4243
                     /locus_tag="ACGI8A_003490"
     CDS             3128..4243
                     /locus_tag="ACGI8A_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HFH0181454.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4395..5264
                     /locus_tag="ACGI8A_003491"
     CDS             4395..5264
                     /locus_tag="ACGI8A_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HFH0181455.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSRRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         2063 a          546 c         1002 g         1751 t
ORIGIN      
        1 tatattgttt gattattatt caaacacacg tcgttaaaaa ttttataaat ttatgtttag
       61 atatgattaa tactcttgat tttagaaata aatatttgta gattgtagat taaagataat
      121 ataaataaat agaaggtaga ttttttagat atattaatat aaagattata taaaatattg
      181 aaatatagct gtttaaatta ttgtctataa atataaaatg aatggtggtg agttaagtga
      241 ccgatttagt taacaccatt aatggaattg tttggtcacc cgtattagtt gcaatgtgtt
      301 tgtgtatagg attatatttt tcattgaaat taaaattttt tcaaataaga gatgtaaaag
      361 aaatgtttac tctcttgctt gagggagata gttctcatgt tggtatatca tcatttcaag
      421 gttttgctac atctatggct ggaagaatag ggactggaaa tatagctggt gtagctgtag
      481 caatagcaat gggaggtcca ggagcattag tatggatgtg tataatggct acattaggtt
      541 cagcaactgc atttgtagaa tctactttag ctcaaatata taaagatgag catgatgggc
      601 agtatagagg aggacctcca tattattttg aaaaaggtct tgggtggaaa aagtgttcag
      661 ttttattttc aatagttgca ataataagtt atacattttt tatgcctggt acacaatcaa
      721 acactttata tctagcaatg aatcaagcat ttggattttc aaaaatgaca atagcagtag
      781 catcttctat tgcattagcg ataataattt ttggaggaat taaaaggatt ggtttatttg
      841 ctgaaaaagt agtacctata atgggtggag cataccttat tatgacatta gtaataatta
      901 taacaaatat tagtttagtt ccagaggcta ttgtaacaat gtttaaatct gcatttggat
      961 ttggttcagt ttttggagga atgctaggtt cagcaatatc ttggggagta aaaagaggta
     1021 tatactcaaa tgaagcagga gaaggttctg ggacttatgg tgcggcagca gcagaagtaa
     1081 gtcatccagc caaacaagga ttagttcaag cattttctgt ttatgtagac acattattaa
     1141 tatgtatggc tacaggtatt atgattgtaa taacaggaat gtataatgtt acaggtgttt
     1201 gcacagcact accaggtgta gaaccgggac cacaatatgt acagagtgca ataaatacat
     1261 tacatccaca attaggtgat atttttatag caatagtttt attattattt acatttacaa
     1321 ctttattagc cttctcatat atgatggaga ctaatgtatc ttacttagta actagaatta
     1381 aagcagaaaa acatgaaaag tacataatta atgtctgtag atttatgctt atagcatgtg
     1441 cagcaatgtg ttgttttata gaaccactta caagctgggc attaggtgat atatgtatag
     1501 gtgtattaac atatataaat ttaattgcca tctttacact aaaaaaacca gctataaagg
     1561 catattatga ttatataaga cagaagaaga tgggaataaa aaggagtgat agaactttta
     1621 accctataga gttagatata agaaatgctg agttttggga aaatagatac ttaaaagagg
     1681 ataagcatga gttaaattaa agttataata aaatataaaa attactaaat aaatttagtg
     1741 ggaggtaaga ttggtatgaa agaaagaata atacaagtgg ctgattcaaa gaaagaaaaa
     1801 attctagggc tatgtcaatc tttatatgat gaaccagaaa ttgctttgca agaatataaa
     1861 tctgctaaaa aaatatcaga atttctaaga gaagagggtt ttgatgttga ggaaaacttg
     1921 gcaggtatgg ctacagcttt taaagcaacc aagaaaaatg gagatggtcc taaaatagca
     1981 tttatagcag aatatgatgc tcttccaggc aatggacatg catgtggaca tcatttaatt
     2041 gcatctatgg gagttggagc tggaatagct ctttcaagta tactggatac ttacaaggga
     2101 gaagtaagta taattggaac acctgctgaa gaaactggag atggaaagcc ttacttgata
     2161 gaacatggtg tatttgatgg ctatgatgca gcaatgatga tacatccaaa ttcaaaaact
     2221 tgtgtaacac ctgaaattat agcaatagga ggtctagatt tcatatttac tggaaaagca
     2281 tctcatgcag gagctaagcc atataacgga ataaatgctt tagatgcagt agtactacta
     2341 tataataata taaatgcact aagacaacaa ttagtagatg gaacgagaat acatggtatt
     2401 atattagaag caggaactgc tgcaaatgta atacctgata tgggaaaagt aagattagag
     2461 ataagggcaa aagaacaaaa ttactttgat gaagtagtag aaaaagtgaa aaattgtgct
     2521 agaggtgctg caatagccac tggatgtgaa cttgaatttt atcactttga accaacttgc
     2581 caaggattaa atgaaaataa agttttggta gacatattta caaagattat ggaggaattt
     2641 ggaatatatg aagatgaaca agtaatgtta ggttcaacag atatgggaaa tttaagtcaa
     2701 ataatgccat gtatacatcc tttgatgaag ttttctgaaa atggagaaga actacataca
     2761 aaagaatttt tggaggcttc tataaattca tatgcaaaag atagagtaat tgatggtata
     2821 aaaatattag ctttaacagg atttaattta tttgaaaatc cagaattatt gaaaaaaatg
     2881 aaggaagagt agaacttaga ctatttttaa ttatactctt taaaataaga agagtgtctc
     2941 aaaatgaact ttttagttta tgagacactc ttttttgtat gaaattaaat atatttttat
     3001 tattttaaac atgaaaaaga ggcttatctg tttttatatg acctttttta acttgtagca
     3061 caaaatatca tgaatgatta caattcgaat gataaaatcg attataagat atataggagg
     3121 ggtacatatg gaaactttag aagtaagaaa tattgaattg agtggtacaa attatgagat
     3181 aggatacaga ctaggagaat tggttgctaa tatgccagag ataatagaag gtcagattaa
     3241 taaatctaat ctggtaagca aaaaagaaga aaaagagatg atagagttgt ttgataaata
     3301 ttgtccaggt cttaatgaag aattgcaagg ttttgctgat gcaattcaag tgaattgtaa
     3361 tcaaatttta tattatacta tgacttatct taaaccaggt tgtagtcaag ttgcattagc
     3421 accagaatta actgaaaatg ggcatgtact atttgcacgt aatttcgatt tctcacacaa
     3481 tatggaggat tttgttttat gtaaaactaa ggtaaatgga aaatatgcac atataggtac
     3541 taccattatg caatttggaa gaggtgaagg tatgaatgaa tgtggcttag gtgtaagtca
     3601 gtcatcatgt ggtattcctg taggcaattc agatggttta agaaaaccag caattgtagg
     3661 tttacagttt tgggctgtta ttagatattt gttagaaaat tgcaaagatg tagatgaagc
     3721 attagaatat ttaaaagata tgccaattgc atacaatatt aatttattgc ttgcagataa
     3781 gtctggaaac attgcacttg tggagacttt agatgggaaa aaagaagtaa atataatcaa
     3841 cagtttagaa agtaaaagag aatatttttt acactctact aatcatacac atattgataa
     3901 acttcataaa ttagatccac aatcaatgaa aaattctatt catagatata aattaattaa
     3961 agaatatata aataagtcta aaaaaattgg agagaaagag ttaatgaatt tattgtcttc
     4021 aaaatatcca aatggcttat cttgtaatta ctataatgat ttttttggaa ctttaaaaag
     4081 tatagttatg gatttaaata taggaaagtt taatatttta tggggtggac tagagaataa
     4141 atgggagagc tactatttaa aaaatgacat taaatctata acacagagga taaacataaa
     4201 tattgaaaaa gcaccatctg acttttttga tttcattgaa taaaaaatgt atttaattat
     4261 tctaaataat taggacatat aaatcaacta aaagttttta tttatcgtat tgaattagtt
     4321 ataatatttt agtatagtaa aaaatagtgc tattttacat atttaaagag tttaagtaga
     4381 agagaggtgc catattggat tacttaaagt attttgaaaa agctgtaatg tatattgaga
     4441 ataatttaca tgaaaatata actgtaaatg atgtggcaaa agaaactgga tattcttatt
     4501 atcatttaac acgcttattt aagtctatgt ttggtgaaag tgtaggaagt tatataaaaa
     4561 aaagacgatt agtaagtagt acaaaagaac ttttatatag tgataaaaaa gtgatagata
     4621 tagctattgg tagtggattt gaatcttcag aagcatttag cagagcattt aaatctatat
     4681 acaaggttag ccctatagaa tatagaaaaa atagaattga tgtatttgtt ggtaaaaaga
     4741 aaaaattaga gttagatttt atgagacatc ttgttggaaa tataacgatt aaaccagtta
     4801 taaaagaaat agaaaaaata aaggtaatag gtataaagga taaagtaatt ttagaaaata
     4861 attcattacc tgacttgtgg gagaaattca gaaaagtaca tcatattgtc ccaaatactt
     4921 tgccatcaag gagagttttt ggaatctgtg aagcaatctc tgaaatacac ctagttagtg
     4981 aaagtatgga gtttaatgag ataataggac ttgaagttaa tagttatgat gtgattccaa
     5041 attcatttgt ttcaaaaact attaaggaag gaaagtatgc tatatttact catactggta
     5101 gcttagataa tttagacaaa acttatgaat atatatgggg tacttggttt ttaagttcaa
     5161 aagaagaact tgatataaga gatgattttg aagtatatga tgaacgattt ttaggtccta
     5221 gtaatgtgaa ctcacaaatt gatatttata tacctataaa ataatctata agaaaactaa
     5281 aagataattt gattaagaaa tagatgttcg taaaagagca tctatttttt atttttataa
     5341 gatttagata attttaataa ta
//
LOCUS       DAYATN010000193         5354 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.197, whole genome shotgun sequence.
ACCESSION   DAYATN010000193 DAYATN010000000
VERSION     DAYATN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.197"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            101..1006
                     /locus_tag="ACGI8A_003492"
     CDS             101..1006
                     /locus_tag="ACGI8A_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HFH0181456.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            1015..2199
                     /locus_tag="ACGI8A_003493"
     CDS             1015..2199
                     /locus_tag="ACGI8A_003493"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0181457.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            2314..3615
                     /locus_tag="ACGI8A_003494"
     CDS             2314..3615
                     /locus_tag="ACGI8A_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFH0181458.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            3569..4612
                     /gene="holA"
                     /locus_tag="ACGI8A_003495"
     CDS             3569..4612
                     /gene="holA"
                     /locus_tag="ACGI8A_003495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFH0181459.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(4799..5065)
                     /gene="rpsT"
                     /locus_tag="ACGI8A_003496"
     CDS             complement(4799..5065)
                     /gene="rpsT"
                     /locus_tag="ACGI8A_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFH0181460.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT         2157 a          473 c          831 g         1893 t
ORIGIN      
        1 aacaatataa atgaaattaa taccataata aaattggagt tttttatata tacaaaataa
       61 aatttttaaa attaagcaat aaaaaaggta ggaggatttt atgtttttca aagatattga
      121 actaaattca aaaaaagagt tagacccata ttttgattta gtagattatg aagcttgtga
      181 gtactgtttt agtactttat atatgtggca acatgtttat aagacaggat attatatagg
      241 tgaagatttt gccgttttag ttggggaata cgaaggagat agcttctcaa tattaccact
      301 tgctaaaaaa gataaacttc cagaagttgt agattttgtc cttgaatatt tttctaaaaa
      361 taataaaaaa atatacttaa gaggtattac tactgaagta gtagagtttt taaaagaaaa
      421 atatccaggt agatttgagt atatagaaga aagagattta tttgactata tttatgatgc
      481 agaaagttta agaactctag ctggtaaaaa gaatcaaaag aagagaaacc atataaacta
      541 tttcttaaaa gagtatgcag gtagatatga agctaaatta ttagataaag aaaactttga
      601 tgagtgttta gtactaatga aggaatggga aagtaataag gaagaaaata atgaatttga
      661 tgaaagtatg gatgatgaat taataggtat caaaaagata tttaaccatt atgacatatt
      721 aaaagataag gtaaaagtgt ttggtgttta tgtagatgga aaattagaag cttttagtat
      781 aggagagctt ttaaatccaa atatggcatt gattcatata gaaaaagcta atcctgatat
      841 aagaggtctg tatccattta taaatcaaca atttttggta agtgaattta aggacgttga
      901 gtttgtaaat agagaagaag atttaggtat tgaaggatta agaaaagcta aactttctta
      961 tcatccttgc agatttgttg aaaagtacag tgttagggag gcttaataga ctatatggag
     1021 attaggtatg ctaaagaaga agagatagaa agtataaaag agatttggag ttattgtttt
     1081 aacgatacgg agagttttat gaagtactat tttaatgata aatataaatc tgaaaatact
     1141 gtagtagctt tagatgaagg aaaaattatt tcttctcttc agctaaatca gtataagtta
     1201 ttactaaata gcaaggtata taatacatca tatgtagtag gagtttctac acttccagag
     1261 ggaagaggag ctggatatat gaacaaagta atgaaattta ctttaaacga attgtataaa
     1321 aaagggcaat tagtttctat attgatgcct attgactata gactttatag aagatttgga
     1381 tatgagcatt gttacgacca gattgaatat actattaaca cagatgattt aaaaaatttt
     1441 aagtcaagtg gaaaaatgat aaagtctaat ttatcacaaa ttgatgattt gatacaaata
     1501 gatagaactt ttttaaatga ggttaatggt aatgtgttaa aagatgaaca ttattatgaa
     1561 aacttattta aagagattca aagtgaagat ggatttttat acattcatga aggaaatgaa
     1621 aaagatggat atatagttta ttttcttcag gaagataaaa tgtttgtcag agagttattt
     1681 tataaaaata tagatgccct taaatcaatg cttaaattta tatataatca caatactcaa
     1741 tgtaagatag ttactatatc tactcctaca atagataaga taagatttat attagacaac
     1801 cctaaagata gtgatataaa aataaaacct tttatgatgg gaagagttat aaatgtaaag
     1861 aagtttattg aagatataga tattgagaat gatattaata gctcatttaa tttattaata
     1921 gaagacaagt ttatagatga aaataatggt ctatttaaaa tttctataca gaataagaag
     1981 gtatctgtag agcagttaga taaaaaaggt gcagaaaagc ctcaagaaga ttttgatatt
     2041 aaattagata taaatacctt aacacagtta tcattttctt atatagatgt aaatgaagct
     2101 attttcttaa atgatataaa agatgtttca gaagaaacac tagaaacact taattgtatt
     2161 ttttctaaaa aaaataacta tataaatgaa tatatatagg tagagtacgt taaaagattt
     2221 taaaaatctt ttgacgtatt tttttgttta gataacaatt tatcttgttt aaaaattttc
     2281 tatttgaaaa taatctaaat aatatacttt tatatgggag tgattataat tcaaataaga
     2341 agaccattat taattatatt aatgtttgtg gtagctatat catttatcat tacaaataac
     2401 acaaaaaacg acgaagaact taatgataag ataataacta tacaaggagt tgtcaaagga
     2461 aaaattgaaa agaaaagata caatcagtac aaggtagggg tttttctaat aaatgattat
     2521 acaaaaaata aaaatttgaa gattggtcag aaagttaata ttacaggtaa attcaaaagt
     2581 ttagataaaa tgaagtatga tgattttgat tatggaaggt atataaaaag tactggatat
     2641 aaaggtatag tgtatataaa taactataaa attattgata aaaataaact ttactcttta
     2701 ataggggaaa taaagtttta tattagtaaa acttataggt acttatataa gaaaaactca
     2761 gattttataa attctatact attagcagaa gttgaaaatt tgactgagga acaaaaagaa
     2821 atttttacta gaactggaac tagtcatgta atatctatat caggtcttca tacaggaata
     2881 ttatgtgtaa ttatatcctt tctattaaga ggtataaata aattgtataa attattaata
     2941 ctgtcgattt ttataacttt atattgtata atggtaggtg cgtctccatc cataattagg
     3001 tctatagcat ttgttatgat tttttattta tcaatattta ttgatagaaa gaaagatgga
     3061 atatcggcat tgtctctaat tgggataatt ttaattatga ataaccccta tgttatatat
     3121 aatgtaagtt ttcaattgtc ttttttagct acactttcta tattaatatt ctataataaa
     3181 ataaatagta ttataaaatt aagtatggta tcattgacaa tatcatcaaa tatattaact
     3241 ttacctataa tttattatac atttaaaggt ataccattgc tttcaattat aggaaattta
     3301 attatagtac catttgtggg agtaataatg tatttaagta ttgcaagttt aatcgtattt
     3361 aaagtaagtg tagtaatagc taaaattata tcttttttta atagtacatt tatagaaagc
     3421 atattttttt tactagaaaa aatcagcaat ttaagttttg catacattaa tatagaaaat
     3481 cctaaatttt atattgttgt tatttattac ataggagtgt ttttttacat attttacata
     3541 gaaggaaaag agataaagga gcaagagaat gaattacaag gatattataa ggaatgtaaa
     3601 agagaaaaac tttgaaaaaa tgtatctttt ttatggaaga gagtattatt taattgagaa
     3661 tgcaataaaa gcatttaaag atagtctaaa cgagggaatg cttgatttta acttagatat
     3721 aatagatggg aaggaaatag tcttaaatca gcttataagc tctatagaaa cacttccatt
     3781 tatggatgat agaaagatag taataataaa agactttgaa ctattaaaag gaaagaaaaa
     3841 gaattttaca gatagtgatg agaaatactt aatagagcat ttagacaata ttccagacac
     3901 cacaactata gtttttgttg tatatggaga tgtagacaaa agaaaatcat tagttaagaa
     3961 aataggaaac aatggaattg tttttgattg tgataagctt tctgatatgg atttgtttaa
     4021 atggataaaa aaatcttttg ctttaaatga tgttataata gataattctc aaatcatgta
     4081 ttttattgaa caagaaggat acagagataa gagtagtgaa aagacattat ctgatttaga
     4141 aaatgaaata aataaaataa gttcatttgt tggtaaaggg aataatgtaa ccaatgatgt
     4201 aattgataaa ttatctcaaa aaaaagttga gaatgatata ttcaaattaa tcgattatat
     4261 tggagaacaa aatgcttcta atgcaatgaa aatattaaat gatatgatac aagaaggcga
     4321 atctgtatta ggtatatttt ctatgatagc aagacaattt aaaataatta tgcaagtaag
     4381 acagttacaa cttgatggat attcaacaaa acttatagct gataaattaa aaatgcatca
     4441 atttgttgta ggaaaagcat taaagcaaac taaaaatttt tctgatgata taattgtgga
     4501 aatattaaac tatatattag aaagtgatta taaaataaag acaggtctta taagagatac
     4561 tttagcagtt gaaatgttgg ttagtagata ctgtaaaaga gaagcaattt aaaaaatcta
     4621 tatattaata aaaaataaat agcaagatat gttatagttc agagtagatg taagctaaat
     4681 taagtttgat atacaatcaa ctataatatg tctaactatt tattttttat tgtataaatt
     4741 tacaaaataa aaaaccccta cataggggtt attatatata agaaatataa actggtaatt
     4801 aagcgttcat accgtttaat ttttgagcta atttagctac ttttctagct gcagcattct
     4861 tatgtatagt tcctttagaa gctacttggt atattctttt ttgagcgtat tggaacttag
     4921 caacagcatc ctctctgttt ccagctgcaa cagcatcttc gaatcttctt attgcagttt
     4981 ttatttgaga ttttctaact ctatttaaag cagttttctt ttcgataaca cttattcttt
     5041 tcttagctga ttttatattt gccaccgtgt tcacctccct gtattttttt atcatattca
     5101 tcgtcaacat attagatttt aacagaaata aatctaaaaa tcaaggatta ttatttatat
     5161 attttaataa tgttttttat tttgattttt tagactgtaa attgtgtata aataatttgc
     5221 aatatcttgt ttttaaattg gtatattttg tgttttaaat attttgacat atataaatta
     5281 atattttatt tttgtaacaa atattttgag ataatacaaa ctattaacat acattttgat
     5341 agtatttcca ataa
//
LOCUS       DAYATN010000194         5352 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.198, whole genome shotgun sequence.
ACCESSION   DAYATN010000194 DAYATN010000000
VERSION     DAYATN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.198"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(204..1388)
                     /locus_tag="ACGI8A_003497"
     CDS             complement(204..1388)
                     /locus_tag="ACGI8A_003497"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFH0181461.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="ACGI8A_003498"
     CDS             1761..4112
                     /locus_tag="ACGI8A_003498"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HFH0181462.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="ACGI8A_003499"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="ACGI8A_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFH0181463.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2097 a          598 c          851 g         1806 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttca tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa ccatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcat ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagagcat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagct
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggtaca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gcttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tattgataca ttatttaata
     4861 gattcttatg ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatatta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaagcat aa
//
LOCUS       DAYATN010000195         5315 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.199, whole genome shotgun sequence.
ACCESSION   DAYATN010000195 DAYATN010000000
VERSION     DAYATN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.199"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            60..260
                     /locus_tag="ACGI8A_003500"
     CDS             60..260
                     /locus_tag="ACGI8A_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181464.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(467..1057)
                     /locus_tag="ACGI8A_003501"
     CDS             complement(467..1057)
                     /locus_tag="ACGI8A_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HFH0181465.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(1060..1935)
                     /gene="dpsA"
                     /locus_tag="ACGI8A_003502"
     CDS             complement(1060..1935)
                     /gene="dpsA"
                     /locus_tag="ACGI8A_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HFH0181466.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(2011..3402)
                     /locus_tag="ACGI8A_003503"
     CDS             complement(2011..3402)
                     /locus_tag="ACGI8A_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFH0181467.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(3406..4542)
                     /locus_tag="ACGI8A_003504"
     CDS             complement(3406..4542)
                     /locus_tag="ACGI8A_003504"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HFH0181468.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(4574..5119)
                     /locus_tag="ACGI8A_003505"
     CDS             complement(4574..5119)
                     /locus_tag="ACGI8A_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFH0181469.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
BASE COUNT         1780 a          875 c          625 g         2035 t
ORIGIN      
        1 aataaaatat atttaatatt atatagataa atttgaagtt tataagaagg aggataagca
       61 tggaaaaatt aaaaacaatt agcgtttttt ctttgattat aagtgtaatt ttaacgattg
      121 gaggtattgg aatagtaact tattatgtag ataatttgtt tattagagga ctatcagtat
      181 ttgttttaat tatgtcctct tcttttgtat caacaacagt tagattgata tttgaagagt
      241 caaaacgata taaattctaa tttaaaaatt ggattaagta aataatttta atatagcaaa
      301 aattacggag attagctcaa aatagacttt ttagtccatg aggcattttt ttgtatgaaa
      361 ttaaatatat tttcatcatt tcaacaagaa aaagaggctt ctctctattt tgagacagcc
      421 tctttgctag tattgtatct aatacataat attataagaa ccaattctat tgatgtggac
      481 tttttataac aggctgtagc tgtttgttct ctagagcaag ttctatagtt ggaattatta
      541 gctcagtatg tgcaatcata gaattaggct ttgctttgca attatcttga ccaaaaggta
      601 caaaatatat atttttagaa ttaaggagaa tgccaatatt tttgaaatta aagcttagag
      661 catcattcgt tgatattgat attactagtg gtttatcatt acgaagatga ccttttgctg
      721 ccattaatac tggtgaatct gtaataccat ttgctaattt tgcagcagta tttccagtac
      781 aaggagctat tattataata tcagcaattc ctttaggtcc aaatggctct gcctcttcaa
      841 tcgtaactat aggtttattt cctgtaagtt catatgcttt tttcataaac tcttcagaat
      901 ttccaaatct acaatcgata ttttgtgata catctgaaaa tatagtgtgt acattagctc
      961 cttctttaac caaattctct aactctataa atattttatc ataagtacaa aacgaaccag
     1021 taaatcctac tccaattgta agtccttcaa gtctcatttc taatcccttc tttcatttat
     1081 aatattttct atagcgttaa caagaatttc accagatgtc tttggtgaat atttaccagg
     1141 aagcccaagg caaagagtcg cattaatccc tgcttcttta acaacagaat aatctactcc
     1201 tccaggagaa gaagctatat ctataatagt tacttcttgt gatacttttt ctaacctttc
     1261 tttcgttaaa atacactctg gtatggtgtt aaaaatataa tcaaattgta aaagtttttc
     1321 atcaagttga cttaaagaaa tactggaata tccaaatgca ttagccgtac ttcttgcatc
     1381 attatttctt gcgccaacac atacatttgc atccaaagcg tataattttt ttgcaagtat
     1441 ttgtgcacat cttccataac ctaatactaa acagttactt tgatgtatat tgattatact
     1501 tcttttaata gcttctgcta tagccccttc agctgtagca actgcattta aaatagcaac
     1561 atcattcatt ttcataaaat cataaatctg aatagagtgt tgctcgcaaa atttacaaac
     1621 tttagttggt atgttacctc caaataatat ttgattagga gataaattaa ttagcaagtt
     1681 atcaatagta gcatcaagac aatcatccgt attttcaata ttaacttgat tctttgtaaa
     1741 tggaattgga cataaaataa tggaactttt ggatagtgct tcagctagtg aagttgattt
     1801 tccaacgatt ccatttataa tttcatttga tatcccataa gtgactacag aatacccttt
     1861 attaattagt ttttttacca tgtacacttg tcgcaggtct cccccaataa cagttatatc
     1921 gtattgattt gacatattgt aagcctcctt tactatatac aatatgtttt tatcaataca
     1981 ttggttcact aaaattacta aatattatac ttataacatt tttaataatg ctaatctatc
     2041 tatcttttca gaagaggtat atggaaatga atttaaaaag ataattttct ttgggatttc
     2101 atttttcatt agattatctt tcattttaat caacaaatct ttcttcgtaa ttttgtctat
     2161 agttattaca aataatacaa tttgatttcc tcttatagtg tctgagtacg gcaatacaat
     2221 tgcatcttca atttgaggaa tttttttaac ttcattttca atcttagtag aactaacttt
     2281 aaatcctcca atatttataa tgtcatcact tcttccttca aatattaagt atccatcatt
     2341 atcataatat ccaatatcat tgacagaata aggtttggtt gcattatata ctgcatattc
     2401 tgtattcaca aaaatttttc cttctctaat atatatatct attcctggaa atggtctgcc
     2461 tacacttaga ggtttttcaa ttagttcatc atagcataaa tatgtaatat aatttaattc
     2521 acttgcccca tagtataaaa taatttcaga ctttggcata tattttttta gatttcttgc
     2581 agtatcttca aataataatt gagaccctgt aaaaatactt acaactttga gtactggttc
     2641 ttttaagtgt ttaaccaaaa gttgaagttt tgtaggtata aggtatatgt tagttacatt
     2701 atattgtttt attgtcctta tccatgattt acaattaaga cctgaactta ttacaatact
     2761 acctccttca tataatactg acattataga atttagattc cctgtaaaac taagtgttcc
     2821 attaacaaac aagattgatt ccccagagat attaaaaata ccattttgta taggaaaaaa
     2881 tccagcccaa ctctcatatg ttctatataa tacctttgga acacttgtag aacctgaaga
     2941 taaagctccc atacaaatac ttttatctaa ataatttata agcttcttat attgatacat
     3001 atatatatta aatttaggat taaaaataga aaaattaact ttttttgcta cttcattatt
     3061 gttatcatca tataaatcaa taacgtccat agattcatca gatataatga ttgaaatatt
     3121 attttttaat aaaatatcat ctaatacttt tttagaaaga ttataatgac aaataatagg
     3181 aatatttttt gaataattaa tagcaaaaaa actaatcaac tgaaacatta tatttttaga
     3241 atatattaaa attgggatat tttcacctaa agaaatttgc tttcctagtt cctcactata
     3301 actcaatata ttttcataag tatatttttc tccatctata attaaaaaat ttttattttt
     3361 atgcctctct ttttttattc ttaaaagttc ataatagttc ataatctaca ccctttcaag
     3421 cagtaaagct gaacctagac caccagctgc agctatagag caaattccat atctaccctt
     3481 agtcttttct aatgctttta aaagatgtaa tgcaataatt gcacctgaag caccatatgg
     3541 atgtccatac gctaaagctc caccaaatat attatatcta tctattaact ctggatactt
     3601 tctttgaaac aacacatcta taactgcaaa tgcttcatta aattcaacag cagatacatc
     3661 taaataatta aggctttcta tctctaaaag tttatccatt gcttgtatcg ctgatgttgg
     3721 acttaaggtt gcatctgttc ctatcgtgca tgtgttaata atttttgctt taggcttctt
     3781 ttttacatgt tctaaatact tcttagaaca tattataata aatgaagctc catcattaat
     3841 aagacatgaa tttgctgcat ttgtaatagt ttcttttcct aaaattgatg gcatcctatc
     3901 taataacctt tgactcatct tatcacgtat accttcatca taggtactat tatttatagg
     3961 agatataata tcttcaagta ttttttcctc tcttgcttct tttgctcttt tatgactttc
     4021 ctttacccaa aaatccaagt ctgctttctc aatttgatat aactctgcta ctctttccgc
     4081 accttcgagc atactatttt ggctattatc atcaggagaa aattgagcaa ctgtataatt
     4141 tggattattt gtattatatc gcttatcatt cttatgatat gtgcgcattg gttgcagaga
     4201 actactttca aaaccacctg caataattaa atcacactga ccagatttaa cctttgaaaa
     4261 agctatgtct atactcatca tagcagaagc acactgcata tccacagtaa aagcaggtac
     4321 ttcatttgac actcctgctg ttaaagtcat taatctagct atattccctc ctgtcccaac
     4381 tgcatttcca catataatct catcaatttt gtcaatttca tactttttaa ttaaatcttt
     4441 taaaacactt gctcctagta attcaggttg aacaaattga aaaattccat tttttagacc
     4501 tatatgactt ctcaatcctc ctaatataaa aacttccttc atacattaca cccttcctta
     4561 tattatagat ttatcataga tttcttctga gtacattgtt cagtttagtg cctaaaaaag
     4621 atgcaataac tgactttatt gtatctccaa ttaaaaatgg tactactgct gcaaaaagtg
     4681 ctgcttctac tgtcatttga ttaaaatagc acataaatac tgttcccata aaatatataa
     4741 taggcattcc aacaaaaatt gttattaata tatatctctt aatatcatta tttcgtcctt
     4801 ttaataaact aataattagt gctgcaaaca aaaacccaaa ataaaaacca cctgttgggc
     4861 ttaataactt tccaatacca gatgtacctc caccaaatac tggaatccca attaaaccta
     4921 tcattatata tacaccaact gtactaaatg cttgtcttgg tgttaaaatc aaagctatta
     4981 aatttatcat tattgtttgt gctgttactc ctgctggagt aaatggcaat ggtatatata
     5041 tgtaagatga tatacacaat aatgctacac atattgacat tttagttaaa tcttgaatat
     5101 ttactttagt atttttcatt tttataattc ccttcatatg tcttatttca tttagtttat
     5161 atgtacatat acaaagcttc tattaaagct ttgttttagt ataaaataaa gacgtttttt
     5221 tgtcaactat tataattatt taggtttaca tttaatttat aagtaaactt tatactgtta
     5281 ctttatattt taatagtcat ttataaaact ttgct
//
LOCUS       DAYATN010000196         5275 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.200, whole genome shotgun sequence.
ACCESSION   DAYATN010000196 DAYATN010000000
VERSION     DAYATN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.200"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(224..2503)
                     /locus_tag="ACGI8A_003506"
     CDS             complement(224..2503)
                     /locus_tag="ACGI8A_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0181470.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2774..4228)
                     /locus_tag="ACGI8A_003507"
     CDS             complement(2774..4228)
                     /locus_tag="ACGI8A_003507"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFH0181471.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4392..5261
                     /locus_tag="ACGI8A_003508"
     CDS             4392..5261
                     /locus_tag="ACGI8A_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFH0181472.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
BASE COUNT         1798 a          828 c          563 g         2086 t
ORIGIN      
        1 ttactatcta aagtattttg ctataatccc atatactctt acctcaaaaa cattcaaatg
       61 taaaaaatac tacatattaa taaactttgc ccaataattc ctaaaataat atcctaacaa
      121 tgatttatgt ttttataata gtatattttt acataaaaaa gtgaacattg attaacaaag
      181 ttcacttttt tataaatttt tatgttataa ttcacaattt tatttaattt gtttcttttc
      241 tatttaaact atatctatct aatattaatt tttcatattc ttctattggc attggtctag
      301 caaataaaaa tccttgggcc atatcacaac ctataaacct taaaaactct gcttgctcta
      361 cagtttcaac accttctgaa acaactttta tatcaagttg ctttgccatt tgaacaatac
      421 ttgaaactac aatttgactc ttttttgcat ttcccactgt tataaagaat tgcctatcta
      481 atttcaatac atcaattggc atatctttaa gcatatttaa agaagaataa ccagaaccaa
      541 aatcatccat agaaatttta aatccaacag atttcaaagt ttgcattatt ttaaataata
      601 tttttgtatt gtcaaatatt gcactttctg tcaattcaat ctcaattaaa tttggttcaa
      661 tttcatattc ttttgttatt ctttcaagct cagaaataaa gcctggattc tcaagatgta
      721 ctcttgataa gtttattgat ataggaaatg tggggaaatt ttcattttcc caacgtttaa
      781 aatttttaca aacttgggta aatacaaaca tatccaattc tgttataaaa ccatttttct
      841 caaaaatagg aataaataca cctggactta ttagaccttt tttgggatgt tgccaacgta
      901 caagtgcttc agcacctaca attttagtag ttgataaatc aaccttaggc tgtaaataaa
      961 ctttaaattg ttcatttttt aaagcatcat gcatttcttg ttcaatatca cgttcttcta
     1021 tgagagtttc ttttaaattt tcagcataaa tctcataaac ttttaaatat tttcttttta
     1081 ctgtagtttt tgcaatcata gctttgtcaa gacaggtaga aatatttgtt tctccctctt
     1141 caacaaaata tacacctatg gatggaatta aattgagttc tactcccaaa cttgttttta
     1201 tcattgacaa ttttttacgt ataagttcac atatttctat aattgactct ctattatttt
     1261 tcttttcaaa tattatagca aaattgtcat ttgaaattct tgaaaataca gattgctctt
     1321 taaatatatt cttaattatc tctgctattt cctttaatac tctatctcct tcttcatacc
     1381 caaacctatc atttactagc ttaaacttat ctatatcaaa ataaattaaa actagattct
     1441 ttttctcatg agaggataaa aattttcccc cttcaagatt aaatttattt gaattgccaa
     1501 tacctgtaac gttatcttta aaagcaaatt cttccaattt ttccttactc tttttatgta
     1561 taaatagtat ataaattgtt aatgccgtaa agattataat caagactaca catgctgata
     1621 atgtaagttt tataatagca tatgatttct cagatatagc agttttagga acttcagaaa
     1681 aaagatacca atcattgatt cctattgggg cataacttaa ataatatcct tttccaaaca
     1741 tactataact taatgaacct gatttaaagt tttttatatt tgacttcata ttgttgatac
     1801 tagattttga tattgaatca cttctgccaa tgtaagaaaa taaattttct ttagtagagt
     1861 tggcgttttt actgcttgga tttaaaacaa tatcaccatt acttctagtt atataagaat
     1921 atccttttcc attaaatgta ggtacagaaa gtattttttc atacaacttc gtactatgtg
     1981 tggcaaatat aactccttta acctcattat ctttataaat tggtacagaa tatacattaa
     2041 tattactatt ggtattttta tcatcttcta ttacatcttt taattttcct gttataacca
     2101 cttctccttg cattgatttt aagaaataat ctcttttaga gaagtcttct atataaccat
     2161 ctgtagtgta tgctgtacca tttggtaaaa taactcccat tcttttaaat gaactattaa
     2221 tagcttgtct ttttagtata gaaaggatat tatctacttc aaaagactct tctccttcaa
     2281 taaatattga aattgactct agtaacttta tatctccatt aatttgtttt tttaacacat
     2341 tgacgctttg atttgctatt tctgaaagat attgattcgt ttcttcacct aatgtcttat
     2401 tcaaccttga accataaatc aaacaggaaa ttgtaattac taagatactt atgacagcaa
     2461 ttatggacaa accataccta ttattttttg catcacttaa cattatgctt cctgcctttc
     2521 tcgaatctat gtatgtataa tcattatctt atgacaatta ttgtaaacat tttataataa
     2581 tacttatttt tccatattta ctgtaattat acaaaattat aaactttatt acaacaataa
     2641 attaattaat atagatatta gtgtttacat tattgcatta tctattttac aattatttac
     2701 atataaaaaa atgtaatatc aaagtgaagc ttgatattac atttttgttc ttgtattttg
     2761 tttaattttt atgctacatc ttacttaaac actcatttgc aacttcttta tcatcaaaat
     2821 gatgcttaac tttacctata atttgataag tttcatgtcc cttacctgtt ataattacaa
     2881 catcatcctt ttttgccata cttatagctt ctgctatagc ttgttctcta tcaacaacaa
     2941 catggtaatt ttcttttttc ttgtcaattc cttcaagtat atcttttatt attgcctctg
     3001 gttcttcagt tcttggatta tcagaagtta taatacataa atcagaatat ttttgtccaa
     3061 ttgctcccat caaaggtctc ttttcagtat ctctatctcc accacaccca aacacagaaa
     3121 ttatttttcc ttctgcaaat tgttgagttg tctttagtac attttcaagt gcgtcaggag
     3181 tatgagcata gtctacaatt acgcttatac ctttatcatt tggaactgtt tcaaaccttc
     3241 ctgatactcc attagaaagt ctaaggcctt ctttatatat tggtttaggt atgccaagta
     3301 catagcaagc agctataact gctaaagtat tatatactgt aaacatccct ggaactggta
     3361 taaatattac ttcttcatat gaaggtgtta ttaatctata agatactcca tccgaatctg
     3421 attttatatc tctagccata aaatcagcct tagtatcaac accataagtg taacaaggta
     3481 catttatacc ttttatattt tcatatatct tttttccacc ttcatcatct atgtttatta
     3541 tatttgccat agtagtctta aaaaataatt tttcttttgc ttttctataa tcctctaaat
     3601 ctttatgaaa atctaaatgg tctggtgtta aatttgtaaa tatgcctagt ttaaagtctg
     3661 tttcatcaac tctatttaat gccaatgaat gtgatgaaac ttccattgca cagtagtcac
     3721 atccattatc caacatatta ttaaaatgat attgtaaatc tatactttct ggagttgttg
     3781 aattggaaac aatgtctttt tctccatcaa aaatttttat agttcctata agtccaactt
     3841 tattcttatt taaagttaat atctcattta gtatagtagt tatactagtt tttccattag
     3901 tacctgtaac tcctataacg ttaaattttt gagatggatg attgtaaaaa ttatcagcta
     3961 cttttgccat atcttttctt gtatctttta cctttataaa tgtacatcct tttattgcta
     4021 catcttcttc aacaagaaat gcacttgctc ccttttctat ggcatctttt atatacttat
     4081 gaccatctga cacaaatcct tttatacata taaataatgt accttttgta acctttcttg
     4141 aatcatattg tacattatta atatctatat ttaattcacc cttgacattg attatatcca
     4201 acccctgtat aatatcattt aatttcataa atgctttcct ccctttaaac tctttctgct
     4261 aaaattacac tacatataat attatattaa gaatttttta ctatgtgaat ggttaaaaat
     4321 gcttgaaaca ttgcaaaaaa tgctctataa ttttctataa tataactgag gaaaggtctt
     4381 ctataaaaac tatggataat aaaatcgggt acttaaacga taactttaaa atatttaata
     4441 ttagagataa aaaagatata caatttgaat accataatca tgattttaat aagataatta
     4501 tatttataaa tggaaatgta acttattttg ttgatggtat tccctataaa ctaaagcctt
     4561 gggatatact ctttatcagc aataatgaaa tccataaacc agttatagat tcaaatgtct
     4621 tttatgagag aattgcaata tggattagcc cagaatttat gaaaaaaaat agtgatgcta
     4681 gtagtgactt agaaaaatgt tttaaaatag ctacagagaa taaatgcaat agattgcgtt
     4741 taaatatgca tgatattgaa tctattaaga gttttattga gcaaataaat atttccgaat
     4801 ccaatactga atttggaaat gaaatattga gaaatacatt atttatacaa cttatgatat
     4861 tcttaaacag aattttccta aaaaatgaaa atatggaatc aacagaagat gttttttatg
     4921 atgaaactat taagaaagta ttaaactata taaattcaaa tatagatgat aagctttcaa
     4981 tagatagcat atcgtctgaa ttttttataa gtaagtatta tcttatgaga aaatttaaat
     5041 ctcatactgg aacgtctata cacaattata tagttcaaaa aagattaatt ttggcaaagt
     5101 cactaataac caatggtcac cttatgagtg aagtttgtaa taaatgtgga ttcaacgatt
     5161 attctagttt tgtaagggca tttaaaaaaa actacggtgt atctcctaaa aattatataa
     5221 acaataaaaa tcctttagat attactcttt tagatgaata gataatttat gtaaa
//
LOCUS       DAYATN010000197         5137 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.201, whole genome shotgun sequence.
ACCESSION   DAYATN010000197 DAYATN010000000
VERSION     DAYATN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.201"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1017
                     /locus_tag="ACGI8A_003509"
     CDS             <1..1017
                     /locus_tag="ACGI8A_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181473.1"
                     /translation="ILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEY
                     EELQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGV
                     IIPSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVK
                     GEFSKINFHGFDYKNWQSSAQITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKI
                     LQFMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGT
                     LFFIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKV
                     WFFD"
     gene            1136..2527
                     /locus_tag="ACGI8A_003510"
     CDS             1136..2527
                     /locus_tag="ACGI8A_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0181474.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            2634..3674
                     /locus_tag="ACGI8A_003511"
     CDS             2634..3674
                     /locus_tag="ACGI8A_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFH0181475.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            4163..4816
                     /locus_tag="ACGI8A_003512"
     CDS             4163..4816
                     /locus_tag="ACGI8A_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFH0181476.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKAAEKIDGIIDGMKF"
     gene            4892..5101
                     /locus_tag="ACGI8A_003513"
     CDS             4892..5101
                     /locus_tag="ACGI8A_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181477.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
BASE COUNT         2097 a          497 c          875 g         1668 t
ORIGIN      
        1 atacttttat cggctacttt agtagcattt acaacaactt caacacttgt aagttctcaa
       61 ggagaagctt ctaaaaataa ttttccaatg gtttactcat atttttcaga gcataataat
      121 gaaaaagaat atgaagaact acaacaaatt agagaagcaa tcaaaggata tgattataag
      181 gagttaagtt ttcctgtact taaatataat agggaagtct tactaagtgt aaagaattat
      241 aacaaattat caaaggaatt gggattagat aaaattagtt caaagttaga aaaatatgaa
      301 ggggttataa ttcctagtag caattcacaa gaacatataa attctttaaa acaattaaaa
      361 aattatagta taaaaaattt aaatatagaa gtaaaaaaat caaaaaataa aataattttt
      421 cctacaggac tgtttagtaa agttgtagtt ataagtgatg acttatacaa tgaagtaaaa
      481 ggtgaatttt caaaaattaa ttttcatgga tttgattata aaaactggca aagcagtgca
      541 cagataactg aaaatttaaa agaaaaacat tttaatttgg atagagaata taacaggtca
      601 tattttttaa cattaccaga tatgtattta gtagagttac aacaaagtaa aattcttcaa
      661 tttatgggtg tttttatagg agtgatatta tttatagcag tttttagttt tttatacttt
      721 agattatata cagacgagat agaagataat gtaaagtaca aaaaactatc aaaaataggt
      781 ctatctttta aaaatatgaa taagattgtt agtatagaga taggtacatt gttttttata
      841 ccgtatttta tagcaattat aaatagcata atttctttac tgtttttaaa tcaagtatta
      901 aataatagtt ttaagttaga aaatttgggt atattactac tgatatcatc tatatattca
      961 ctttattttt acttattaaa aaaaatttat actagaaaag tttggttttt tgattaaagt
     1021 ggtatataat agatatataa attaatattt aaaaagtagt taatattagg ggagggcatt
     1081 tgcacctccc ttgtaatatg tggaaaattg aatattagaa gaaggaggag acttgatgga
     1141 aaatcaacaa ttattaggaa cagaacgaat tagtaaatta ctactaaaat attctatacc
     1201 agctataata ggtatgttgg taaatagttt atataatgta gtagatagaa tttttattgg
     1261 aaatatacca ggggttggac cacttgcaat aacaggtctt ggtgtaacaa tgccaataat
     1321 gacaataata cttgcatttg ggatgttgat aggtatagga actactacta ccatatcaat
     1381 aaaacttggg caaggtaaag tagaagaggc aagaaaactt ataggaaatg caatgacgtt
     1441 atctgtaata acaggaatta tcataatgat acttggaata ttatttgcaa ataaaatact
     1501 tacattgttt ggagcaagtg aaaatacttt aatatatgca aaatcatata tcaatattat
     1561 attattaggt acagtagtaa atcttctttc cttttcttta aaccactcaa taagagctga
     1621 tggtagtcct aaaatttcag caggaataat gatagtagga tgtttaacta atatagtttt
     1681 agactggata ttaatatttg gattcaattt gggaatacaa ggagctgcaa ttgctacagt
     1741 aacttctcag gctcttacag ctattttaac tatagggtat tatataagtg gaaaatcaaa
     1801 cttacgattc agtaaatcta atctaaaatt agataaaaag ctaataaaag cagtgtttgc
     1861 aataggtatg tcgccatttg ctatgcaact tgcagcaagt ttagttcaag taatatcaaa
     1921 tattgcactt aaaactcatg gtggagattt agctataggg gctatggcta ctatttcttc
     1981 tattgcaatg gtatttttga tgcctatctt tggaataaat caaggagcgc aacctattat
     2041 aggtttcaat tatggagctg aaaaatatga tagggttaaa aaagcatatt taggttcatt
     2101 agtagtagct actataattt tatgtatggg tatggtagta gttatgcttt tcccagaagc
     2161 tattataggt atatttaata aagaccctga acttatgaat atatctgtta atggattaag
     2221 aatatactta cttatgttgc caattgtagg cttatcagtt acaggaacaa actttataca
     2281 atctatagga aaagcaaaaa tggctatgtt attgagcctt ttaagacaag taattttatt
     2341 gataccagca gtattaattc ttcctacatt cttaggttta cagggtgtat ggacagctca
     2401 accagtgtct gattttatag ctacagtaat tacaggtata gttgtattta gagaattaaa
     2461 aagatatact cctaaaactg aaaaattaaa tgaaaatgaa agactaaacg agataactac
     2521 tgagtaaaaa tataaattca gataattgag caagaaattg caaaaaatac gattatatga
     2581 taaaataaaa ccgctgtcaa ggataagaga aattctaatt atggagtgat tttatggaat
     2641 ttacaaagaa aagcaaatta aaaaatatgt atgtaaacat gaatgtagct agaatgttgt
     2701 ctaagataaa tgagtataag ggtagacaac tgctttacaa aaaacaacca aaagaaattt
     2761 tagaaaactt agaaaaaaaa tctttagtag attgttctga atctacatac attggaaatc
     2821 aaagagatag tagtaatttt aatctagaaa agttgataag taatgaagtt acacctagaa
     2881 gtagagagga attgagtatt gttgaataca gagatgtggt aaaaactatt aacagtgctt
     2941 atgagagtat tcccatcagt tcacaaacga ttcttgagct tcatggatac ttatataaat
     3001 tttcatctac tagaggagga agttataagt cagataatga ttttattgaa cacaatttaa
     3061 aacctagcga atttataagt attgattcaa gtgtcaataa agttgaaaaa gctgtagaag
     3121 aaatatgtga agcttataat acattgatag aggaagatga aattgatata ttaatattaa
     3181 tttctgcatt tgtgttagat ttcatattga tacatccatt caaagaaggt aatataaaaa
     3241 tggcgagagt acttatccta ttgttactaa ataaaaatgg gtatgaagtt ggaagatata
     3301 taagtttagg aaagatattt gatgatagtt catatgaata ttatagtaat ttaaattatt
     3361 tgaaagcctc aataggtagt gaaaaagcag atatgaatgc atggattgag tattttttag
     3421 agacgatatt aactgcatat gagaagctgg atgatagttt aaatatatct gataagaaaa
     3481 gacaaacaaa aactagtaga atagagaaaa taataaattc tacccttgga tattttacaa
     3541 aagaagatat aagagattta tgtcctgaca ttccagaacc tactataaat agagttttta
     3601 ataatctaag aaaacaagat aagatagaag ttgttgcaag aggaagaagt gcaaaatgga
     3661 agaaaaaata ttaaaaaatg tttagataac taaaatggta tagtgtttta cactatacca
     3721 tttttaatgt cattagcatg agcaataatt taacaactta agcaatatca attttattta
     3781 ttgattattt ttgaactgaa aaatggcgtt atatggtata cctcagtggt aattaatttt
     3841 ggatgagaag atataatatt acagaatata ttaggagtat atgtagaaaa aatataataa
     3901 aagtgtgagg cttagtaatt aacaaattat gtggcttcac acttttttat aaaataaaga
     3961 tacttagcag aagaatattt tagttgatat gtttataaga tggtacttgt ttgggtaaaa
     4021 gtataaatag taaaaaagta gaagtaatgt gtataaaaac gtaagttaca ttctgtaata
     4081 atagttattt tataattcaa caaaagaata gaatgggtat atttgtgaaa taataatata
     4141 aaataatctt aggagtgagc ttatgaagaa aaaattatta tatataaatg taaattcaaa
     4201 acctgaagac ttatcttcaa gtaaaactgt agcaagaaaa tttataaata aatttatgga
     4261 aaaaaataaa gattttgagg ttgaagagat agacctttat aaagaacata taccaagact
     4321 tgagtatcag tactttgaaa aaagaaatag catagttagt gaagaaaatg ctaaaaattt
     4381 agacgataag gatagaaaag aacttacaaa gattagaaat ctttgtgacc aatttgtgag
     4441 tgcaagtgta tatgtaatag cagcacctat gtggagttta tcttttccag caccacttaa
     4501 agagtatata gattgtataa ttcaagatgg aaaaactata tcttttgaag gcaatgataa
     4561 accacaagga attttaaatg atattgatag aagtatggta tatatacaat cttctggtgg
     4621 acacatacca tgggtgttga agccagttat gaataaaggg ttaaattatg tggaaagtat
     4681 tatgaaattt ataggaatta aaaaatttga tgaattgtta gtagatggaa ctggaactag
     4741 tgaagaagaa agacaagctg ctatagaaaa agcagctgaa aaaatagatg gtataataga
     4801 tgggatgaaa ttttaattat aatattttat taaaaatata aaggaaaaac ctcttttaat
     4861 gtagaaactt atattgaagg aggcgagaat catgcgagaa gaaaaaagta atgaaaagta
     4921 tgattgttat tggtgtaatc aagagaataa tttctgtgta gaaataaaag ataatatagt
     4981 catgatagat gatggcactg gtacgttaaa acaagccgtt ttcatagggt ataaacaaat
     5041 ccaaattaat ttaaactgtt cacattgtca aaacttgaat agaataaaat taaatttgta
     5101 gaattttaaa atggaaatta ttgaaggaat taaaaat
//
LOCUS       DAYATN010000198         5120 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.202, whole genome shotgun sequence.
ACCESSION   DAYATN010000198 DAYATN010000000
VERSION     DAYATN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.202"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            322..699
                     /locus_tag="ACGI8A_003514"
     CDS             322..699
                     /locus_tag="ACGI8A_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0181478.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            692..1840
                     /locus_tag="ACGI8A_003515"
     CDS             692..1840
                     /locus_tag="ACGI8A_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFH0181479.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1857..2459
                     /locus_tag="ACGI8A_003516"
     CDS             1857..2459
                     /locus_tag="ACGI8A_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HFH0181480.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2552..3574)
                     /locus_tag="ACGI8A_003517"
     CDS             complement(2552..3574)
                     /locus_tag="ACGI8A_003517"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFH0181481.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3903..4085)
                     /locus_tag="ACGI8A_003518"
     CDS             complement(3903..4085)
                     /locus_tag="ACGI8A_003518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181482.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4398..4817
                     /locus_tag="ACGI8A_003519"
     CDS             4398..4817
                     /locus_tag="ACGI8A_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HFH0181483.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
BASE COUNT         2022 a          614 c          725 g         1759 t
ORIGIN      
        1 cataattaga atgttattaa aatttattag atattactct agtttatttt aaaaaaaagt
       61 attataatat tccttgtgtg aaaatatagg aatttttatt aaatagtttt aatgtgtaaa
      121 taacattcta cactattgta attaaatatg taaaaagtaa aatatttaaa tagatatatt
      181 ttaaaatcta acttttaata ttgtaaaaaa attagaaatt taacaaaaag tacaaaaatt
      241 cacatttttt tcataaagtg tttgactaaa aaattcacat gttgtagaat taaggtagca
      301 ggagttttga gggaggtgta aataatgata agaaaaatac cacaagcaga actaaaagta
      361 atgaaattca tttggaaagt agatgttaca gtgacatcaa aagatgttat tgaagctatg
      421 gaacaaaaat atggctggaa acaaaccacc acactgacac ttttatctag gctggtaaaa
      481 agaggattct tagatgctca aaaaatagac agatatacac attacacaat aagagtaaaa
      541 cacaaagatt atttaagttt tgagacaaag gatttcctaa gtaatataca tgatgactct
      601 ttaagaagct taatttcagc attacatgat gatgaaaata taggtaaaga taagctagat
      661 tttctagaaa gttattttga taatttaaag gatgaataat tatatgctat atgtttttga
      721 aaatcttata aagacaacta ttgtatgtag tcttggtatt tgtctattat tatttttgaa
      781 aagatattta tttaagaagt ttagtaagag attcaattat tatatttggt tgataatagt
      841 ctttagaatg ttactttttc tatttaatta tacaatagtg tatgaagtaa aagaacccaa
      901 agaaaatgca ttaggaaata atattacaca aattgatatt tctacagata ataatcttat
      961 gctgtatgta gcttatttat ggttatttgt aactatagtc attgcagttt atacatttat
     1021 aaagtataca agatttaaaa atctagttgt tgatgtatct tatgatattg aagataatga
     1081 tataaactgt atctacaaaa acttgttaaa agaattaaat ataaaaaaga aaattgaatt
     1141 aagaggttct gatgaattga taagtccagc aggtatggga ttatttaaat cttacatatt
     1201 cttaccagac tatccttata gtaaagatga actaacttgg attttgaaac atgaattaat
     1261 gcattttaaa aataaagata ttttaataaa gtttttagtt ttgtctgtta gaataatata
     1321 ttggtttaat ccacttgtat atgtaatgag caataaagta aatctagatt gtgaattatg
     1381 ttgtgatgaa agtgttttgg tagaatgttc tttaaaagat aaaaaagaat atgcactagc
     1441 acttataaag tcaattaagt ttagtaaaaa ttataatagt aagattttga caaccgagtt
     1501 tgataagaca aatttggaaa agagacttga gagtattgtt aaaaagaaag gcaaaagtgg
     1561 aatactaatt gctgttttag tatttatgac ttctataaca tcttttgttg aagttgatgc
     1621 tcaagttaga attgatgagg gtaataatgg aatgaataac aatattaatg aatttttatt
     1681 gaataagacg actgataaaa ttataaatat ttattataaa gatgcaccaa atgaaattag
     1741 gaaattttat gaagataaat gcgaattaga aggtaaaata cctagagact atgacataat
     1801 agaaatggat atgaaagatt ataatcaatt aataaaataa ttataaggag gattttatgt
     1861 taacaaaatt aagaaaaata gtagctatta tgtgttgctt attgcttact tttacaagtg
     1921 tggcatttgc tgacacaagt aaagagaaag gaggaagtaa agagactgtt ccagagaaaa
     1981 acctagattc ttttactctt tctgggtata tatatacata tgaaaatgcg ccacaagaaa
     2041 taagagatga acataaacaa aattgtgaag agataaatat agaccctaag ccagatgatg
     2101 agatatttgt tccagaatca gctaatctaa tgaatgagga caatagtaaa ggtgtttatg
     2161 caagttatac tgtttcttat aatataggtg ctaaaactat aacaattatg agtaatgagt
     2221 atttgactat aagtacaaca aaagttatac gaaagggtaa tagcggaaaa gaagtaaaag
     2281 ctgcacaaat catgttaact ttacttggtt ataatgtagg tatagatagt tcttttggaa
     2341 gtaagacata taatgcagtt gtaagtttcc aaaaaaaata cggtttgtct gcagatggaa
     2401 taattggtcc agctacttgg gataaattgg gtagactaac tgaccctact ctttcatagg
     2461 attaaaatct aaaaatatat taaatagtgt ctcaatatga tttttcaatt taaatgagac
     2521 actatttaaa tataaatgtt ataaaactat tttacaataa attccatata actctaccat
     2581 ctatatcctt aacattttga caaccagtca aaatcatagt ctcacataac tcattcctaa
     2641 ccttctctat aaaaaactcc acaccatcaa gaccaccgcc aaaagaagca gtaacaaaag
     2701 gtcttcccat caaaacagca tcagcaccca atccaagcat cttaactaca tcaacaccag
     2761 tcctaactcc accatcaaca agtattgtta tctttccctt tacagactta gctatatctg
     2821 gaagtacctc acaagtacca ggagtataat caagtactct tccaccatga tttgaaacaa
     2881 taatggcact agcaccagac tctacagcca tcaaagcatc atctacagtc ataattcctt
     2941 ttataataaa aggtaatcta gtggattcaa ccaattctct aatctcatca attgttttag
     3001 gagaaaaagg attttcttga aattgattat taataagtcc acaagcatca aggtctacac
     3061 caactgcaaa agcacctgct tcttcagaca atctaatttt atctattatc tttgaattat
     3121 tccaaggctt tataaataca attccatttc ccctattatc cttcaataca tctaaattat
     3181 ccagtaaaaa agtatctaca ttagtatcgc caaccatagc ataaattcca gaattagaac
     3241 aacctctaac aacaggctct atgtattctt tttcggaaac tttaccaccc atgttcaata
     3301 tagtacctga tacaggtgca gcgaatatag gagaactcat ttttctacca aataattcta
     3361 tggatgtatc tggctttgaa acattatgta tgactctcat atttatcttt attttttcta
     3421 gactttttct attttcaatg aaagacgaac cacttccttt accacccata ccaggaacat
     3481 ctccagcaca agctaatcca ttacaaattt tacatacttt acatgttcca ttaaagttct
     3541 ctctagctga ttttagtaag tcattgtaat ccatacaaaa tctctccttt ttataatatt
     3601 aaaattgagt attactatat tgaaaatatc ttaattgtaa atatagggat gttattatga
     3661 tgagctaaac tagataatac atattagata ttttttattg ttgtagatag ttaaattata
     3721 tcatattaga tagtcaaatt ttgaaaaaaa ctaacttatt taagtataca ttttgaaaaa
     3781 attaaaaaag ctatatatag atttttaaag aaatctatat atagctttta agtttatgtc
     3841 aatctttttt gttaatgaat aaataatata gaatacaata tacaaaaaag tttcttgaaa
     3901 tgctaagata atccccattt acaaagcaac atcattatac tgtgtaaatt tgaatcaaca
     3961 atgttttcac catatttcat cataaaatcc tctaattgtg tgtcaataaa tgaataatat
     4021 ccacttatac tatcgatgta tgcagtcatt aagtgtatac attcatcact tttatcttta
     4081 tacatatata tctcccccta tatacaatat ataaatttag ataccaaatc gtatcatatt
     4141 aaatagttaa gtgtgtaaca tctatgcagt tatatattta tatcattata ttaataacta
     4201 aagtgagttt tgacaataga ttttgggaaa ctggatgttc aaagtgcaaa cttggatttt
     4261 aaaatcatta attaagatag cgcataggaa tatatgtata cataacttaa ttataaagat
     4321 tataaacaat tacttttatt aactttattt aaatggtaga ataacttgga gattaataat
     4381 tataaggagg atataatatg agtttatata agtttatata tgatgataag gaatatttat
     4441 taaaagagga gaactgtagt gctttaataa atgatgaaga aaatccagtt caaggagtaa
     4501 gtatttcaaa aataattgat atacttaatg aagcagaaga agttgatttt gatgtagaat
     4561 attatcaaga agcatgtcca ttatgtttag aaggagtaaa agaaaagaaa aaattctttc
     4621 cttttttaga atatcacttt tatattttca gtaaagatgg agaatatgta ataagcaata
     4681 tatctgttga ttataaagga ctatctttta ataaactatc aagggcaaat aaagttgata
     4741 atagttatat tgtaagtgtg ataatttgcg aaaattgtca ggattatata gttcagatag
     4801 aaaattgtat agtttagtgt aaagtatgtc ttaatttaag agaaatttct aaattgagac
     4861 atcaactttt ttcaaaaata gtatggaaaa ttactaaatt tatgagataa tgtaagtcgg
     4921 aggtgagtta caatatatag taaatatttg gaagagatag atgatacaat tttagattca
     4981 atgtttaaac taaccttgaa tgaaagatta ttgactaata acgattttat tgaaaataaa
     5041 attgatgtag taaagaattt aattgattgc tataaaactg aacaaaatag tttaatgtta
     5101 ttaaaatcaa attttttgct
//
LOCUS       DAYATN010000199         5035 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.203, whole genome shotgun sequence.
ACCESSION   DAYATN010000199 DAYATN010000000
VERSION     DAYATN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.203"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..739)
                     /locus_tag="ACGI8A_003520"
     CDS             complement(<1..739)
                     /locus_tag="ACGI8A_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181484.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIF"
     gene            complement(740..1615)
                     /locus_tag="ACGI8A_003521"
     CDS             complement(740..1615)
                     /locus_tag="ACGI8A_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0181485.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLSFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(1619..1987)
                     /locus_tag="ACGI8A_003522"
     CDS             complement(1619..1987)
                     /locus_tag="ACGI8A_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0181486.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(2170..2829)
                     /gene="deoC"
                     /locus_tag="ACGI8A_003523"
     CDS             complement(2170..2829)
                     /gene="deoC"
                     /locus_tag="ACGI8A_003523"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFH0181487.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(2865..4052)
                     /gene="murJ"
                     /locus_tag="ACGI8A_003524"
     CDS             complement(2865..4052)
                     /gene="murJ"
                     /locus_tag="ACGI8A_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFH0181488.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(4129..>5035)
                     /gene="tnpB"
                     /locus_tag="ACGI8A_003525"
     CDS             complement(4129..>5035)
                     /gene="tnpB"
                     /locus_tag="ACGI8A_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFH0181489.1"
                     /translation="EPDKFSLQNALKDLDNAYKKFFKEKAGFPKFKSKKINRFSYKTN
                     FTNGNIMYCGQHIKLPKLGMVKIRDKQVPKGRILNATISKEPSGRYYVSLCCIDVDIE
                     VFENTNNHIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRN
                     KARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDV
                     SWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNE
                     THDRDINASINILKEGLRLITIQNK"
BASE COUNT         1657 a          852 c          506 g         2020 t
ORIGIN      
        1 taaatattat gcaaataact ataattccta cttttgttcc aaataaaata aaaggattct
       61 gaaatgaata aataattttt tctaaataca atagcatttt atgcatattt ttagttgcaa
      121 aattaactat ataaattatc attaatatcg gtaatcctat caatatagta atactagtaa
      181 cttttctaac tctatatgta aaagctccaa gtaacactcc tataaaacct atacatattt
      241 catttataca tatttgtaca aaataattta aataagttag tggcatactt gatgttgaag
      301 gatctatcag tgctgatata gatgtccaaa tcatatctct ctttagtaaa actgatagtt
      361 ctatactgta acctgttata atttctataa ataacttcga taaaaaaatc aaaactatac
      421 ctaaaataga aatcacaaag cttaaaataa ttaaatctat gacaatagct tttatacaac
      481 tttttctatc tgctcttata cttaaagctc ctgaaaagtc tttatttgct atatttactc
      541 catacacaaa tagcagtata gttatatatg ttaaaatagg attcacaatc gtggataaat
      601 tagttttaga tgcttctcca aaaatcttga gaataaacac tggcaaaata ttcataacaa
      661 ttcccacaat cataaatata attatataca atttagtatt tttattaaaa aattttatat
      721 atggctttaa ttcattcaat tacaattcct ccttcttagt catgttaaca aacatatctt
      781 gtagccctat gtatcctaca tcaatatttg aatttttaag tattttttca tcactttcac
      841 taaaatcacc atagtaaaaa taagcaactg catttccaaa tgccttttta ggttttatat
      901 tttttataca ttctaatttt tctaactctt ctttatttcc agtaatataa tgagcttttt
      961 gttttatgac atcaatttct tcatctataa taatttttcc ctcatttaat attattacat
     1021 gctccagtaa atcatctatt tcatcaatca aatgagttga tattattatt gtccttggat
     1081 tttttatata actatctaaa attatgttat aaaactcttg tctatttact gcatccagac
     1141 caattgttgg ctcatcaaat atggtgatag ctgaattaga acaaatacct attatatttg
     1201 aaagtatagt tttcatacct cttgacagtt gtttatactt ttttttagta tttaaatcaa
     1261 aatatttgca taattcttct tctaagcctt tatcataact tttataaaaa ctagaagaaa
     1321 attcaaatat ctgacctact ttaaaatctg gactataaaa ttctttttcc ctaacgatac
     1381 aaacttcttc caaaactttt atatcttctt ttgggttctt tcctaatatt tctatctcac
     1441 cttcattttg aattaactga tttaccatta tatttaataa tgttgttttt ccagctccat
     1501 tcttacctaa taaaccatat atcttatttt tttcaaagct tagagacata ttattaaaaa
     1561 tattttgctt cttaaaactg tgagataaat tttctatctt aatagaacta ctcatatatt
     1621 aatcctcctt gaaattaatt ataatatcaa ccaattcttc tctatttatt tctaatttgt
     1681 tagcttcttg aagtaggttt tttacaaaat tatgtttaaa attttctttt ctagcttctt
     1741 ttataatagt ttttgcacca tctgatacaa acatccctat ccccctcttc ttatataaaa
     1801 tatttttatc aaccaatata tttatacctt ttaatactgt agctggatta attttataaa
     1861 gctttgaaag ctctgttgtt gatggaacct gttcttcttc ttttattcca tttgatagta
     1921 tctcatcttc aattgctcgt gctatctgaa taaatatagg ttcttcatta tttaatacta
     1981 atttcatagg ttcctccttt ctccatcagt tagttactca actaattaac tatataactt
     2041 ataatatctt gaaaaatatt ttctgtcaat actaaataaa aaacagaggg aaaattccct
     2101 ctgttttttg ttctttatat ttataattaa attttgattt ttgtaatcct atcccataag
     2161 ttaaaaactt taataatctg ttgtatcttc attttttaat attttacata ttgagctagt
     2221 tcctaacctt tctgctcctg cctctataaa tttttctgca tcagaaattg atttaactcc
     2281 ccctgcagct tttattttta cattttttcc aacatgctct ttcataagtt ttatatcttc
     2341 taaagtagct ccacctgttc ccattccagt agatgttttt ataaaatctg aacctgacat
     2401 tgttactatt tcacacattt ttattttttc atcttcatct aaataacatg tctctataat
     2461 gacttttaat actttatctc ctatagcttt ttttatttct tttatttctt ttcctatatt
     2521 atcataatct ttatttttta tatctgaaat gtttacaacc atatcaactt catctgctcc
     2581 attttcaata gcatcttttg cttcaaatac ttttgtagca gtagtttgat atcccaaagg
     2641 gaaccctatc actgtacata tctttatttt accttttaga tattctgatg ctcttttcac
     2701 atatgatggt ggtatgcaaa ctgatgctac actcatatct acagcttcat cacataaaat
     2761 tttaatatct tcccatgtag ttgtagcttt taaaatagta tggtctactg tttttaatat
     2821 atgtttcata aaatcctcct aaatttataa ttaatataat tagattactt ttttaaatgc
     2881 aattttctct ttgcaatgtt tagtatcata tcaacctctt caactttaaa aataatcatt
     2941 agtagtgtat aaattccacc tccaactata actgaaatag ctagtgatac aaactcatta
     3001 aatgttccta ctcccaaaat tccaaatata aatttatacg taaagtatga taaaacaccc
     3061 ataattaaag atgcaactac cgatttcaaa gttgctttta ttattttatc ttgtccaaaa
     3121 tatccaactt ttctctttag gttcaagaag agtaacaata tacatgcaat tgatgatgaa
     3181 ctagttgcaa gagcaagacc accatgagcc attggtttta ttaaaactaa gtccaatact
     3241 atatttaccc ctacagatat aatcccattt accataggtg tttttgtgtc ttgtaatgaa
     3301 taaaaaacct ttcccaaaat atctcttaag ccaaatgcag tcataccaac tgcataaaaa
     3361 attaaggcag ttgctgtcat ttgggttgcc ctcgcatcaa aagcacctct ctcaaaaatg
     3421 attctaacaa caggagttgc aaaaaaaata gaagcaactg aaataggaat catagaaatt
     3481 attatcatat ttatactgga tttaactgaa ctagtaaact tcttttgatt attttctgca
     3541 cttaacttag ataacatagg atacataaca gatactattg aagctgtaaa tgtccctgta
     3601 acaaaaccat ttaatctatc tgcataagta agtgctggaa tgcttccttt aactaatgtt
     3661 gaggctaaag ttgtatctac caatgagttc acttgattaa cagcaacacc tataaatact
     3721 ggtgaaagta atgctagtaa tttaataagg ctatcatctt taaagtttaa gtagtataag
     3781 tatttgtagt tcgttttttt aacaaaaaac atgtaaaaga gcagttgaac taccatagca
     3841 accacagcac caattggaag tatgtatggt ccaaatatgg tacttaacat tattgaaata
     3901 attattacta tattataggg tatacttcca aatccaacta caataaagtt ctctttaatt
     3961 tgtaaaaatg ctgacataac actcgtaata cctatgaata ttattcctgt tctgaacctc
     4021 ccatgactaa agtcacaggg ttcctgcttc atagaatttt gcatactaaa atatgtctta
     4081 ctaattctcc acaggctatc cccgtagttc ctacggttct tacatacgtt atttattttg
     4141 aattgttatt agtcttaatc cttcttttaa tatatttata cttgcattta tatctctatc
     4201 gtgagtttca ttacaactag gacaaatcca ttctcttata tttaagttct taacttcctc
     4261 gtttttatac ccacatttat tgcatatttg tgaacttgca aagaatttac ctacctttac
     4321 aatttgtcta ccataccaat tagccttgta ttctaattga cgaataaact ctgaccatga
     4381 tacatcagaa attaagcgag atagtttacg atttctaatc atattcttta cttgtaaatc
     4441 ttctatgcaa ataatatcgt tctcttttat taatttagta gatagttttt gtagaaaatc
     4501 atttctttga ttagctatat gttcttgaag tcttgcaact tttaatcttg ctttatttct
     4561 atttaaacta ccgattgttt ttctcgataa ttctctttgt aatttagcaa gtttgtttaa
     4621 tgacttctta agatacttag gattttctat aaattctcca caacttgaga tacaaaattc
     4681 tttaattccc aaatctaaac ctatatgatt attagtattt tcaaatactt caatatctac
     4741 atcaatacag cataatgata cataatatct accacttggt tctttagata tagtagcatt
     4801 aagtattctc cctttaggta cttgtttatc ccttatctta accataccaa gtttaggtag
     4861 ttttatatgt tgaccacaat acataatgtt tccatttgta aagttagttt tatatgaaaa
     4921 tctattaatt ttctttgatt taaatttagg aaatcctgct ttttctttaa agaatttttt
     4981 atatgcatta tctaaatctt ttaaggcgtt ttgtagtgaa aatttgtcag gttct
//
LOCUS       DAYATN010000200         4960 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.204, whole genome shotgun sequence.
ACCESSION   DAYATN010000200 DAYATN010000000
VERSION     DAYATN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.204"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            17..820
                     /locus_tag="ACGI8A_003526"
     CDS             17..820
                     /locus_tag="ACGI8A_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181490.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESALKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            832..1962
                     /locus_tag="ACGI8A_003527"
     CDS             832..1962
                     /locus_tag="ACGI8A_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0181491.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            1966..3375
                     /locus_tag="ACGI8A_003528"
     CDS             1966..3375
                     /locus_tag="ACGI8A_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFH0181492.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3592..4095
                     /locus_tag="ACGI8A_003529"
     CDS             3592..4095
                     /locus_tag="ACGI8A_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HFH0181493.1"
                     /translation="MFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPIYS
                     NNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRGGK
                     CEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVSSY
                     ISSHIPQ"
BASE COUNT         2068 a          424 c          859 g         1609 t
ORIGIN      
        1 gaaggaaagg cacattatgg aaaaattgag agttattgaa atgataaaaa ataatcccaa
       61 tattatagct acaatagata atccaactga tgaaatgaaa ttgttagcta ttaaagaaaa
      121 tgggcttgta ctagaatata taaataatcc aaccagagag atgcaagaac tagcaataga
      181 taataatatt agagcaatta aatttataga taatccaaca gaagatatga tgataaaagc
      241 agtaaatgag ggatggagta tactggatta tatcaaaaac ccaactgata aagttattga
      301 aatggctata aatcaagcag gatgggcaat taaatatgct aaaaatccaa gtgaagaatt
      361 acaactgtta gcagtaagaa aaaattatga ttcgataaag tttataaaac aaccttatga
      421 aagtgtacaa gaggaggcag tgagaataag ttatgatgcc ttgagatata tagattcacc
      481 tagttataat gcagaactta tagctataaa aaataatgaa gcagctatta gctttataac
      541 tgatttagat aaaaataaaa tgttggaatt tttaaaagtg aatattttag tgattaagta
      601 cattctgaaa aaagtatcta aagatgaatt agagagtgca ttaaaagaag tattatccaa
      661 agaagatgtt gaggaaaagt atattagaga ttttttgaat tgtagtgtta tagatagaaa
      721 tagcgaaagt ataccaatag ataaaattat gtttatttat aaatatggaa gtaagacagc
      781 aaaaaaaata gctgtagatg agaaattaaa gattatgtag gagagagaaa aatgaatttt
      841 atagacacat taaaaagcgt tgacttaata ttatccactg gagaagtacc tttaatagta
      901 ggtgaaagtg gaataggaaa aacagcttta gcaaataagc ttgccaaaga aaatgattgg
      961 agtttaattg ttattgatgg aaacttactt aaagagggtg aaataggtgg tcttccaact
     1021 atagaatctt atgtaggagt taattctaat ggatataaga ctgaaaagaa aacaacagtg
     1081 tatgctgttc ataacaaatt aagagaaatt gatgaagaga tatctaaaag taagacagtt
     1141 cttttattta tagatgagat aaatcgttgt gagcatacag tacagcaaga acttatgaat
     1201 ttaattttaa atagagaaat taatggatat aaactgcatg atgatgtaaa gattttagca
     1261 gcaatgaatc catcaagtaa atatggttca gattttgatt atcaagtggt agatatggat
     1321 gcagcccaag aaaataggtt tgtttggtta aatatggaga gtgaccatac acaatggata
     1381 aaatgggcaa tagatgaggg tattgaaaga aaagttatcg agtttatttc cacttttcca
     1441 gaatatctgc ataaaataaa tgaagatgat gtaagagcca ctcctagaag ttatgagaga
     1501 gtatctaaaa tttataaagt ttataaagaa aaaaataatt ctattcctag agcagtattt
     1561 ttaaatgttg taaagggtaa tgtaggtaaa gttattgcag aagaatttat aagttttatt
     1621 gaatcaaact ctgaaccatt aatatcctat gaagatgttt ttttaggaga atctattgat
     1681 gagtctattg tagaaagagt aaaaaatgag agtcatacaa gactttatct atcagctatg
     1741 aacattctaa aagacttaga attaaatatt aagaatgata aatatgaatc aaaccactat
     1801 attaatagat ttattgaatt tttaaaaatg tatcctgtag atttaatgat aggtattatg
     1861 aaggatatta gaaacagtta tattgaagta tataaaaaag ctatagaaaa tgaagagttt
     1921 gtaaaatcat attttgaatc ttatagttta ataaggggat aatgtatgga aaattatttt
     1981 gataaacaag caaaagagct ttataataaa gcaagtgaaa ttataaatac tcattctatg
     2041 ctaaaggcaa atcgtgctaa tgagaaattt gatatagata taccacaaga ttttaagtat
     2101 gagtttttta gtcttgtaga taaagtgaat ttaagtctta tggaagaaga agataatttt
     2161 tatggatatt ttttgtttca gatgtcaaga gaaataagat ttgatataag tagtcctaca
     2221 ggtgtgaatt ttaagggagc taaatatatt atatatttta atccaataat ctttttaacg
     2281 cttgacataa agcaaatgga gactacaatt aaacatgaaa ttcatcatat attatctatg
     2341 catttgatga gagcaaagga gttaaaagga aagtatagta cattagcaat taatatggca
     2401 atggatatag tagtaaataa atttctaaac aatttaccac catatgcaac taccttagag
     2461 tgggtaaatt taaaatattc cttaaaactt gaaccatatg aaccttttga atattatgta
     2521 gaaaagattc agactgaatt agatttgatg gaagtggatg aagatggtga ggaagatgat
     2581 agtgataaat atggagatat agaaattgat tatagccccg aaagaactca tgatatatgg
     2641 gatgaatcca atgagataga tgaaaatacc cttagagaat ttactgaaaa atttattaac
     2701 agttctcaaa aaggtaaagt tcctgcttat ttagaaagta tgatatcatc acttaaaaat
     2761 agtaagggtg aattgccatg gaatttatat cttaatagaa taatgggaac agttgaaaat
     2821 aataaaaaga agactataac aagaagaaat agaagacaac caaatcgttt agatttgagg
     2881 ggagaactta gaagtcataa gtcagaaata gcagttgcac ttgatgtaag tggaagtatt
     2941 agtgatgaag agtttaaaca agctattaaa gaagttctta gtatagtgaa aaactataat
     3001 catgaaatta caattataga atgtgataat gaaattagac gtgtatataa agtgagaact
     3061 gaaaaagata taaaagatag aattaggata ggtggaggta ctagattttc tccagtcttt
     3121 gagtatgcta ataataaaaa gattaatttg ttagtctact ttactgatgg taaaggtgaa
     3181 aataaactac aagtaatacc tagagggtac aaaattttat gggttatttc tggaagagga
     3241 gataaaattt cattaaaaga accttatgga gcagttaaaa aacttactaa ggttaaaaca
     3301 aatgatgaaa tattagaaat aaatgatgta agaaatgatg ggtactcaat gaatagccaa
     3361 gcaccaattt tataggtatt cacaggagat aagctgtatt aatttatttt gatatgcttg
     3421 ttataaaaat ctaaaaaata cattaatcaa tggttaatgt attttttaat tttaataata
     3481 taagttttac ttacatttaa gtctcaaaat gtgttaaaat ataatatata gcagatagtt
     3541 ttggcaaggt ggatttagtt gtcaaaacta ttttttttat taaggatggt gatgtttttg
     3601 tttaagattg atgattatat aatgtatggt atgacaggag tctgcaaagt catggatata
     3661 acaaatgaac gatttacaaa tggaataaaa aaagagtatt atgtattgag tcctatttat
     3721 tctaataata cagtaataaa aattcctgta gataatgaaa aagtaccaat gagaaagtta
     3781 ctttctaaaa ttaatgtgct ttcattaata aatgatattc ctaatatgga tacatcatgg
     3841 atagataatg aaaaattgag aagtgagcaa tttaaaaaaa tactcagagg tggtaaatgt
     3901 gaagagttaa taaaattagt aagaagtata gataataaca gagagtatgt aaaatctatt
     3961 ggtaaaaaaa cacatcaagc agatgataat ataatgaaag aagctgaaag gttattaaat
     4021 gaagaatttg caactatttt agatatttct cctaatgaag ttagttcata tatttcaagt
     4081 catatacctc aataaagtgt tttagtatcc tttatttaat ctattttaag tattatttat
     4141 gtatgctggt tttggtaaaa taaaatatat aattaaatta tataaaaaat aataatgatt
     4201 gtattgaaga attacagaat agtgcggaga gcctactcct ttaggtgtga gatggatatc
     4261 acttaaagat ttaaattgtt attgtagatg tttttaatat aaaacaagat taataagtgt
     4321 attgtaagct ttaagtgaaa ttgatactat aatgagttac ttagatttta aaatatataa
     4381 tattataaaa aatcctttta ggttttgaat gaataagatg gatactatgt tgaatagaat
     4441 ccatcttatt taagttttat ttatgttgtt tatttgtaag cattattttt gtacatgaaa
     4501 acacagtgta aaaatatgct tagaattatg aagttttatt aaaaagatat tcgacaaaat
     4561 aagactaaaa atattcttaa acttataact aagataaata ttgtatattt atagtgtagc
     4621 ctgaaaaatt gtggatgtac gaaatttatt tagtatttta ttgaaacaaa gtagaagaag
     4681 tactatagtt tatctttgaa tagattgtta ttttttagtg aaaaagttat taggttaata
     4741 cataaatata atagcttata aatagtttaa gcatgttaaa aaagtagtta ttagagatat
     4801 atagttacat tgcttataag atacttaaaa tgatagcatt cacttatatt cagaagatta
     4861 agataaaaaa tcatttttat atagtaaagc atacctaaat ttagaaaata attgaattta
     4921 taaaattaat taaatacata aaaaagtatt caatcaactt
//
LOCUS       DAYATN010000201         4887 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.205, whole genome shotgun sequence.
ACCESSION   DAYATN010000201 DAYATN010000000
VERSION     DAYATN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.205"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            115..1266
                     /locus_tag="ACGI8A_003530"
     CDS             115..1266
                     /locus_tag="ACGI8A_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFH0181494.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1312..2487
                     /gene="thiI"
                     /locus_tag="ACGI8A_003531"
     CDS             1312..2487
                     /gene="thiI"
                     /locus_tag="ACGI8A_003531"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFH0181495.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEESTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2643..3446
                     /locus_tag="ACGI8A_003532"
     CDS             2643..3446
                     /locus_tag="ACGI8A_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFH0181496.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3689..4330
                     /locus_tag="ACGI8A_003533"
     CDS             3689..4330
                     /locus_tag="ACGI8A_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HFH0181497.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1957 a          463 c          932 g         1535 t
ORIGIN      
        1 taatatttta taatttaaga ttgtcttttt aataaagatt aacaaaaaaa atactataat
       61 tagtggtata atagttagag ttgaagattt taaataaagg taggtgtaat taagttggaa
      121 atatacttag acaatagcgc aactacaaaa ccttatcaag aagttataga taaaatggtt
      181 tatgcactta atacggaata tggaaatcct tcttctgtcc atagaaaagg tgtagaagtt
      241 gaaaaagcta taaaagaagt aagacaagat atagctaaat ctctaggagc gaaagaaaag
      301 gagatttatt ttacatcagg tggtacagag tgtaataata ctataataag aggtattact
      361 agtttaaata aaaaaagaaa aaatcacata atatctacta acatagaaca tccttctgtt
      421 ttaaatacat taaaagattt agaagaagat ggttttgaag taacttattt agaggtcgga
      481 aaagatggga aaataaatat agaggattta aaaaatgcga taaagtcaac tacatgctta
      541 gtaagcatga tgcatgttaa caatgaaata ggaactatac agccaatagg tgaagttgga
      601 aagtatttga agggtttaaa agaaaaaatt tatttccatg ttgatgctat tcagtcatat
      661 ggaaaaatta actttagacc atctaaatac aatatagatt ttatgagtgt aagtgcacat
      721 aaatttcatg gtccaaaggg aattggattt atgtatataa aagaaaataa tcgccttaag
      781 cctatgttaa caggtggtgg tcaggaaatt ggaattaggt cgggaacaga aaatgtacct
      841 ggaatatatg gtcttggaga agctgttaga atactaaaca aagatttaga tgctgttatt
      901 tcgaaagtag ataacttaaa aaatatatta aaaaatgaaa taattgataa tatagaagat
      961 ataaaaatca attctccaga agacggagta tgtcatatat taaatgtatc atttagaggg
     1021 acaaaaggag aagttttact tcattactta gagcaaaaag gtatttatgt atcaactggc
     1081 tctgcatgtt cttctaaaaa gaaaggtagt tacgtattaa atgctatagg tcttacaaat
     1141 gaagaaataa atggaacaat aagatttagt ttatctgata tgaatacaga agaagaaatg
     1201 ttagaagctg tgaaagtttt gaaagaatca atttgtgact taaggtcaat aatgaaaaga
     1261 aaataaagaa tactgtataa atagtgataa taaattagtt taagaggtga aatggtgtat
     1321 aatatattaa tagtaaagta tggagaaatt ggagtaaaag gaaagaatag atatatattt
     1381 gagaataggc ttataagaaa tataagaaat atgttaaaac caattggaag atttaatgta
     1441 tataaagagt atggaagaat atatgttgat ttagaagact atgattatga agaagtaatt
     1501 gaagaagtta gaaaagtatt tgggatagtg ggtgtgtgcc ctggagtaag agctaaaaaa
     1561 gattatgata cattaaaaga aatagcttta aaaatgttag aagaaaaaat agaggctgga
     1621 tataaaactt ttaaggtaga gtcaagaaga ggagataagt ctttcggatt gacttctcaa
     1681 gaaatgagta tggatatagg tggatactta ttatcaaagg taggagatag gattaatgtt
     1741 gatgtaagaa atccagaagt taaaataaag tgcgaataca gagaatttca tactatggtc
     1801 tatagtgata ctatacctgg atatggtgga ttgccacttg gaacgaatgg tagagctatg
     1861 tctcttttat caggaggtat agatagccca gtagctacat ggatggttgc aaaaagaggt
     1921 atggaagtag aggcagttca ctttcatagt tatccattta caagtgaaag gtcacaggaa
     1981 aaagttaagg atttagctaa aatactggca aaatactgtg gtagagtaag gcttcataaa
     2041 gttaatatct tagaaataca aaaagcaatt ggcgagaatt gtaatgaaga agaatctact
     2101 attctttcaa gaagatttat gatgaggata gcgcagagac tttctgaaaa aagacattgt
     2161 gatgctttaa ttacaggtga aagtattggg caggttgcat ctcaaactat acaaggactt
     2221 acttgtacta atgccgttgt agacttacca gtgtttagac cacttatagc aatggataaa
     2281 tcagatatag ttgatatagc caaaaaaata gggacttttg aaacatctat agtgcctgaa
     2341 gaagattgtt gtagtgtatt ttctcctaga aagcccgtca ctaaacctag attagagaaa
     2401 atagaaaaat ctgaaacagc acttgacatt gaaaaattag tacaggatgc tattgacaaa
     2461 atagaggtag aggatattga gttttagcat acaagttatt taattactaa attgaatatt
     2521 gagtatatta gtagttctgt aagtatatta gtagttctgt tgaaaggatt tatgtagaca
     2581 atttattaat atgtagtatt taaaaataag tactataatt ttatttaatg ggaatgatta
     2641 ttatgaataa aaagggtttt acactaattg aattgttagt agttatatct ataataggca
     2701 ttttagttat agtagctgtt ccagcattgt ttagaaatat agaaaaaagt aaagcagtta
     2761 catgtctttc taatagagaa aatataaaga ctcaaattgt tattgcaatg gctgaggaat
     2821 caagtaaagg caagaatgaa gtcatgaaag aggtattaga aaacaaagat ggtaagtact
     2881 ttgaaacaga accaaagtgt aagtcaggtg gaatatattc agcaacgttt gatgatggtt
     2941 atgatggaat aactggaata gaaagcattg caaaagtgta tgttacttgt acaaaacatc
     3001 cagatggtgt tgaaatggct agggatatac atcaaagtat gaaagatttg attgcatcat
     3061 ttgcacaaga cccttctata ataccaggag cctcaaaggg taatgatgat tttagaaaat
     3121 atttattaga caataaatat aaaaatgggt ggcctacaat tccagatgaa tttaaggcaa
     3181 aatatggatt aagtaaggat acactatata tacaaccata tgcatataat cctactaaat
     3241 ctgatgctac tgtagttgta tttgcaaata ataagactgg gggtaattgg tatacttccc
     3301 tagtttacga ttatgatgaa ggtagatggt ataaaggtaa aaatggtatt tctgttgcag
     3361 gtaggtcatg ggatgttgac acagatagtg ttaagtctgt aaaaacagag attcattcta
     3421 aagagggatg gggtccttta aattaatata tcatgtttta ttaatctggt attgattaaa
     3481 ctatagaaaa gagcagtttc tatagatgac gaataccaga ttttcattta tatatataat
     3541 tgagatatat taaaagtgtt tatattttta agtcttcaca tacaagctaa aaataattat
     3601 aataatattg taaagaaatt taattataag attaattttc tgaagattta gtatttgtac
     3661 taagaaatac ataaaaaggg gaatagatat gaataataat tttcaagaca accaaacaat
     3721 aaatttaata caaagtcgta gaagtatacg aaaatttacg acagaacaaa ttagtgatga
     3781 gcaagtaaac acattattac actgtgcatt tgcagcacct tcaggatgta ataaacagcc
     3841 ttggcacata actgttgtgc aagaccagaa acttttaaaa gagattagtg atgatacttt
     3901 atctaggatt catgaggtga gtaatgttga gataaataaa aactttaagt tattttatgg
     3961 tgctccaacc gttttattta tttcttatga tgaaagtagt tcatgggctc catatgatat
     4021 aggaatactt acagggaata taacaacagc tgcacaagca ctagggcttg gaagttgtat
     4081 aataggaatg gttagaggtt tatttacacc tgttgaacaa ggggatattg aaggtcttgt
     4141 gagtgtattg gataaagaag atgtaaaaga atcagagtca ataaagatga agtttgatac
     4201 aaataaaaaa tacagagaac tacttgatat accagaaggg tattctgtac catttggtat
     4261 tgctgtaggt attccagatg gaaatttacc aaatgctaga gaggtagttt acaaagtttc
     4321 cagagtttag gataaagtat tgcaagaacc aatcagttac ttgaagaatt aaatcagtaa
     4381 tatacaaata aattgatgga agaggaagct atttaaaaag tagtttagat ttttcgttgt
     4441 aaaataatta aataaagaat aaggtgataa gtattttatt tattatctta ttctttttta
     4501 tttttgtttt gtagaatgat aaaaataagg attaagtgta ataaaataga aaacaagtat
     4561 gttaatttta agtatatgct ttaaaattta tccaagttaa aaatttaaga tatcttttca
     4621 gtataatgga aaaaatgtca aaatattgaa atagggaaaa aatagtgata taataagaaa
     4681 aaatagagaa aaaaagtaac ccttagttat aaaaaagtta ctatttattg aaaatttaga
     4741 tatttttcta aaaattatct atgttaatag ttaaatttat gaacattttt tgtttttatg
     4801 ttaggatatt tcttagaaat gaaatacttc ttaatttgat ttgatatgta tatttttata
     4861 gctattttat tataaaaaaa aagaacc
//
LOCUS       DAYATN010000202         4855 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.206, whole genome shotgun sequence.
ACCESSION   DAYATN010000202 DAYATN010000000
VERSION     DAYATN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.206"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            529..1389
                     /locus_tag="ACGI8A_003534"
     CDS             529..1389
                     /locus_tag="ACGI8A_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFH0181498.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKTLNIKASDYLSLTLLDHLEF
                     LLKRCKKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSE
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1379..3250
                     /locus_tag="ACGI8A_003535"
     CDS             1379..3250
                     /locus_tag="ACGI8A_003535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFH0181499.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQSTTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3270..4724
                     /locus_tag="ACGI8A_003536"
     CDS             3270..4724
                     /locus_tag="ACGI8A_003536"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFH0181500.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYEMPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLGKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         1869 a          511 c          891 g         1584 t
ORIGIN      
        1 aactaaattt aagtatttat atattagtat atcttactag catttaaatg aaactaaatt
       61 tgaagcttta cctattaata tatcttaata gtgattaaat aaaactaaac ttaagacttt
      121 atatgttaat atatcttaag aatttatttg ttaaaataca gttcattata tttaaaagca
      181 ttactatgat aattcataat agtaaaatag ataatggttc agacaatatt ataaatacta
      241 gtgacggtaa aatagtatca atttagtata taaaccttgt cagataatcg cataacagag
      301 cttgtagtta tagaataaaa aaatatctag gaaaccgttg acagatttat atggatagta
      361 taacatatag aatatgaaat taaacaaaaa agaattttaa ttatggcgac gtagtcataa
      421 ttttgagtat gttactgatt agcaggcaga actcaagtat atatgtgaaa aatagaggga
      481 tactctatac atatatgctt gagttctttt tattgaaggg aggaatatat gaaaataatt
      541 cagattttaa ataataatgt agccttagta aaaagaggta aaaatgaaat tattgtattt
      601 gttaaaggaa ttggctttaa aaaaagagtg ggacaagtaa taaatgaaaa tgaaatagag
      661 agaacatata tactagattc atacgatatg ctagaacact ttagttatct actacaaaac
      721 tcagactcca ataatatagt tcttattaat gaaataattg aatatggtga gaaaacttta
      781 aatataaagg caagtgatta tctaagctta accttactag accatttaga gtttttactt
      841 aaaagatgta aaaagaatca atttataaaa agtcctttgg tatggaatgt taaaagattt
      901 tatccaaagc attttgaaat tggaatgcat gcactaaaat taattggtga tagtaaggga
      961 attaatcttc ctgatgatga ggcagtatca atagcacttc attttgtaaa tatggaggta
     1021 aataaagagt ctcatgatag tacaattgta gagttgagaa ctttagcaga tatagtttct
     1081 ataataaaat atcattttaa tgtagagcta gatgaaactt caacaaatta tatgagattt
     1141 actactcatc tacagtactt tatccaaaga attatgtcag aggaagcttc aatagatgaa
     1201 gataattcta tagatttata taaacaagta agtcaacttt atccaaagtc atttggaagt
     1261 gttcaaaaga taagaaaata tataaaatca caatttaatg tcgatatatc tataagtgaa
     1321 gaaacttatt taatgttgca tataaatagg gtgactcaaa gactggaggc aaaatcagat
     1381 gaaatataaa gatttaaatg aaaatataat aaaacttata gggggaaaag aaaatataaa
     1441 gtcggtagcg cattgtgtta ctcgtttaag atttacttta aatgatagga gtatcgctca
     1501 aacagaagaa attaaaaaat taaaaggagt aattgatgta gtttctaatg atgttgctta
     1561 tcaaattata ataggtactc atgttgtaga tgtatataac gagtttatgt ctatgatagg
     1621 tttaagttca agtgaatcaa gtataaaaag tgaaagaaaa aagggaatta gaggtatcaa
     1681 aggtatattt aattccatct ttgtggtagt ctcagagact atgacttcag taattgaagt
     1741 actacttgca gctggtatac ttgcaagtat attagcactt ttaaatatgt ctggcatact
     1801 tcagagtgac agtcctacat atcaaatatt agatacatta agaagtgcta catttcactt
     1861 cttaccagta ttaattgcaa tagcatcagc aaaaagactt aatgtaaatc catatatagc
     1921 agtagtatta gcagttacgt tattatcttc atcaatagat ggtgtagaag gattaagttt
     1981 atttggtata aatttacagt ctacaactta tgcaaacagt tttattccaa tacttttagg
     2041 agtatgggca atgggaatta taattgaagg tttgaaaaaa atattaccta aagcactaca
     2101 ctatttttta gttccagtat tatctttaat aataactcta tcagttacat taactgtatt
     2161 tggacctatt ggtatgtgga ttggtgatgg attaaactat gcatgtatgt ttttaattga
     2221 tacattgggt aattggagtg ttgttgcact gtatgcagct ttacaaccac tcttaatagt
     2281 ctttggtgca gcaaacttta ctatgcctat atctttaaat tctgtgacaa cattggggta
     2341 tgacccaatt tttatgggcg ctgcaacaat atctgatatt gcagtttgtg gtgcaatgtt
     2401 tggatatttc ttaaaaacta ggaataaaga acaaaaacaa ttatttggta cagtaagttt
     2461 tagtgcactt atgggagtta cagaaccttc agtgtttggt gtgtttatga aatatagaag
     2521 accatttgta gcagtaatga taggtggagg tataggtggt ttaatagcag gtttagctca
     2581 agttaaaaca tatggaatgg tttggggatt agctgcatta ccaacgtatc ttgttggagg
     2641 agaggtatct aactttacat ttatgataat aagtgtagta gtatcatttg tagcagcaac
     2701 agcagcttcc tatttactag gaataccttc agatgaaaaa gaagaagtta agggtgataa
     2761 tgaaaaagaa ttagaactaa taatcaacga gaacaattca agaactatta gtattggaaa
     2821 tgttgcaaaa ggtcaggtaa ttgcacttgg ggaagttaat gacaaagcat tttcctctgg
     2881 agcattaggt aaaggggttg gagttatacc tagtgaatca aaatcaactg ttatatctcc
     2941 tgtagatgga actataacta tagtatttcc aactaaacat gcatatggta tagtgactga
     3001 tgaaggatta gaaatattaa ttcatattgg gatagatact gtaaacttag atggaaaatt
     3061 ctttgaaagt cttgtaacac aaaatcaaag ggttaaaaaa ggagatgctt tagcagtatt
     3121 tgaatctgat aaaattatag aggaaggctt tgattctact ataataactg tagttacaaa
     3181 tactagcgat tatttagatg taatctcatc aaatgatgaa tcagaagaat tattaactgt
     3241 aatagtataa attttaggag ggaaaattca tgaatattaa agatacattt ttctggggag
     3301 gaagtattgc agcccatcaa tgtgaaggtt catgggatag tgacaacaaa ggacctgcaa
     3361 ttatggattt tgtaactaag ggctcttatg aaatgccaag agtaattact gataaaatag
     3421 aagaaaaatt ggattatcca tctcataatg gaatagattt ttataataga tataaagaag
     3481 atatagcttt atttaaagaa atgggattta gtgcattaag aatatctata gattggtcaa
     3541 gaatttttcc aaatggagat gatgaaaatc ctaatgaatt agggattaaa tactatgaag
     3601 gtctgataga tacattaata gaaaataata tagaacctat tgtaacacta tatcattttg
     3661 aattacctat gaatttagta cataaatatg gttcatggaa taacagaaaa cttatagatt
     3721 tgtatttaaa gtacagtgag actgtaataa gaagatttga tgataaggta aagtactggg
     3781 ttacatttaa tgagatgaac catattgacc ctcaaacaga gcactcggat atttttactt
     3841 atatgatagc aggtattaag tatagtgaaa tagaaaataa ggttcaaaca ttggcaaatg
     3901 tagggtataa tatgactttg gcgggtgtaa aggttgcaaa gttggctaga gaaatcaatc
     3961 ccaacaataa agttggatgt gtatttggac taaatccaat ttatccatac aattgtaatc
     4021 ctagtaatgt gttaaatgca tttttagata atgaccgtga ttattatcaa attgatgcaa
     4081 tgtgcaatgg gaagtttcca caatataaat taaaagaata tgaaaaaaat aatataacta
     4141 ttgaagtgac tgatgaagac aaaaaagcat ttaaagaagg taagttagat tttataggac
     4201 ttaattatta tttttcaagt gtatctactc caaaagaaaa tttatcaggt gataaatctt
     4261 tatttggtgg gattcaaaat ccatatttgg aacaaagtaa gtgggggtgg gcaatagacc
     4321 ctataggttt aagatttacc ttgaactata tttacagaaa gtatcaatta cctatcttaa
     4381 ttacagaaaa tggtttaggg gcatatgata aaatcgaaga agatggaact ataaatgatg
     4441 attatagaat tgaatattta ggtaagcatc tatctcaaat gaagtctgca attgaagaag
     4501 ataatgttga atgttttgga tatttgatgt ggggaccaat tgatttagtc agtgcaacta
     4561 caggtcagat gagtaagaga tatggattta tctatgttga cttagatgat gaaggcaaag
     4621 gaagttttaa gagatataag aaaaaatcat ttgattggta caaagaagta ataaaatcta
     4681 atggagaagt tttagatttt tctacggata tagtagtagg gtaggaataa gataaataga
     4741 agttaggaac taatgttaat tagtgtctag taaagcattt aaaatagagt atagggtctt
     4801 taaagtaaag tgtgtattct tcttaaaagt aatggcaact aaaattacta taaaa
//
LOCUS       DAYATN010000203         4838 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.207, whole genome shotgun sequence.
ACCESSION   DAYATN010000203 DAYATN010000000
VERSION     DAYATN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.207"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..824
                     /gene="bclA3"
                     /locus_tag="ACGI8A_003537"
     CDS             <1..824
                     /gene="bclA3"
                     /locus_tag="ACGI8A_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HFH0181501.1"
                     /translation="TGADGATGPTGATGPTGADGLVGPTGATGATGLAGATGATGPIG
                     ATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGATGPTGATGATGATGPTGA
                     SAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAF
                     SMPRAGTITSISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTAVPGATVTLAPPL
                     TGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            993..1754
                     /locus_tag="ACGI8A_003538"
     CDS             993..1754
                     /locus_tag="ACGI8A_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HFH0181502.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            2063..2341
                     /locus_tag="ACGI8A_003539"
     CDS             2063..2341
                     /locus_tag="ACGI8A_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HFH0181503.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            2634..3209
                     /locus_tag="ACGI8A_003540"
     CDS             2634..3209
                     /locus_tag="ACGI8A_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HFH0181504.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            3648..4358
                     /locus_tag="ACGI8A_003541"
     CDS             3648..4358
                     /locus_tag="ACGI8A_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFH0181505.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            4352..>4838
                     /locus_tag="ACGI8A_003542"
     CDS             4352..>4838
                     /locus_tag="ACGI8A_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0181506.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DM"
BASE COUNT         1920 a          530 c          840 g         1548 t
ORIGIN      
        1 atacaggagc agatggagca acaggtccaa caggggcaac aggtccaaca ggagcagacg
       61 gattagtagg tccaacagga gcaacaggag caacaggatt agcaggagca acaggagcaa
      121 caggcccaat aggagcaaca ggtccaacag gagcagatgg agcaacaggg gcaacaggag
      181 caacaggtcc aacaggggca gatggattag taggtccaac aggagcaacc ggagcaaccg
      241 gagcgaccgg agcaacaggt ccaacaggag caacaggagc aacaggagca acaggtccaa
      301 caggtgctag tgcaataata ccttttgcat caggtatacc actatcactt acaactatag
      361 ctggaggatt agtaggtaca ccaggttttg ttggctttgg tagttcagct ccaggattaa
      421 gtatagttgg tggagtaata gaccttacaa acgcagcagg aacattgact aactttgcat
      481 tttcaatgcc aagagctgga acaataacat ctatttcagc atacttcagt acaacagcag
      541 cactttcact tgttggctca acagttacaa ttacagcaac actttaccaa tctactgcac
      601 caaataactc atttacagct gtaccaggag cgacagttac actagctcca ccacttacag
      661 gaatattatc agttggttca atttctagtg gaattgtaac aggattaaat atagcagcaa
      721 cagcagaaac tcgattctta ctagtattta ctgcaacagc ttcaggtctt tcattagtta
      781 atactgtagc aggatatgca agtgcaggaa ttgcaataaa ttagattaga taagaaaagg
      841 cttctaatat cgtattgata ttaggagctt ttttaagaat gattgatgaa catagtttta
      901 atattaatta gtgcgcatat tataaaatac tattatataa tataaaataa taacattaaa
      961 ttggaaatat agaattatct aggggtgata ctatgaaaaa taagattgaa atatcaatga
     1021 acaactttgt aaaaaattat cataaatcaa ataatataac tacaaagtgg caagaacttt
     1081 tgtttgctta tgcatctgct gaagatgata tgtttcagaa gctaaaaagt gttgtaggaa
     1141 catctcatgc tatgccaaaa gattttctgg aaaatggaaa aactataatt acatatttta
     1201 taccttttga tgaaagtgta aataatagta atattggagg gtttgaatgt tctgaaatat
     1261 gggcaagagc gtacatagaa actaatatgt tgatttcaaa tttaaatgat ttcttatgtg
     1321 aagaactaga gaagatgggg gctaattcat atgctattcc tgggtcgtat aattttgaca
     1381 gtgaaaaatt ggtaagtgac tggtcacaaa gacatgtagc attcatagca ggtcttggaa
     1441 cttttggttt aaataatatg cttattacag aaaaagggtg ttgtggaaga ataggaagca
     1501 taatcacaag tttagaatta gagccaacta aaagaccaga aaatgaatac tgcttgtata
     1561 aacataataa tagttgcaaa aaatgtgtca acaaatgcgt gaaagaagct ttaaaagaag
     1621 atggatttaa taggaataag tgttatgaaa tgtgtatgat aaatgaaaaa aaatacactt
     1681 atataggtgg atgtgatatt tgtggaaagt gtatagttgg agttccttgt tcagtaataa
     1741 acccatcaag ataatggatt taagttgact aataatttta aagatagttt ataatgaaaa
     1801 aattttagtt atcaaaataa atataatata aaaactaaat aaatgtgttt aaaaaatgga
     1861 taaataattt gtgttgattg acaaccagtt atactgttga tagacttata tgtatcgctg
     1921 tgatatataa ggagggttga gattttatgt ttgatttaat ctttattttt gcatagcaag
     1981 ggctattgca actatgaaat ttaatatata aatagccctt tgctattcct aatgaaaaat
     2041 tactatttaa taggagagca aaatgagcta taaaaaagca atacatatat taccagaaga
     2101 attacttgaa ttaatacagg aatatgtaga tggtgaatgt atctatattc ctagaaaatc
     2161 taataataaa aaagaatggg gaagtaatac acctactcgt gaggagttgt caattagaaa
     2221 tatgcagatt tacgaagact atcaagttgg atatgatttg caatatttat cggaaaagta
     2281 ttatttatct ttgaaaagta tccaaagaat tgtattacaa gaaaaaagaa aagatatcta
     2341 aggatgagcc agtatagttt tataattata ctggctcatt ttcttttgat aaaagtgttt
     2401 gaggtatagt ttcttttaag tggatgatat aatttaagta atttcaataa cttatacagt
     2461 agctaacaaa gctggttgta aagtatctaa taagaagtag tagcttttta tatgtttgaa
     2521 attaaaattt attaaaatta ggaggtcttg ttttgaaaaa aatacttgat tatattccac
     2581 cttttcataa ctttctatta atttagtaat cttatagaaa ggaaattgca tttatgaaaa
     2641 aaattacttg ggaggtggaa tatatctccc agttacctgt aattaggtat tgtaaaaaat
     2701 gtggaaaaaa gactgaatat atttgttcag gtttatttag ggttaatgct caacataaat
     2761 atttggatat atggttaata tacaagtgtt caaattgtga ttcaacatgg aactcaacta
     2821 tatattctcg tgttaatcca aaaagcttaa atccagaaat actagaacaa ttccatacaa
     2881 acgattataa tcttgttaag caatatgcta tgaatatgga acttttacat agaaatggag
     2941 cagaagttgg tattccagag tataaaatag taggacaaga aattaatatt aatgacctta
     3001 cacaagttca tattattagt aaatatccat gtcagttaaa ggtatcaaca ttattacgtg
     3061 agaaactagg tttatctagg aaggtatttg acgaaatgtt ggattgtggg attattagaa
     3121 gtaactcagg agttgattta aagaaaggta aattaaatta tgaaattatg ttagatatag
     3181 gcaaggagaa gcaaaagtct tcaaattgat tagaataact aacttaatat attttcagga
     3241 acatcattta aaaaaatagt ggctttaaaa aatttaaagc cactattttt taatatgttt
     3301 tatattaata tttaaaatat agtgtgtatt cttcaaaatt atagcgataa ttaaaatatt
     3361 actataaaaa ggaaggtgca tactttttat ttaacaaaaa ctagatatta aaaacttttc
     3421 gaggaatatt ttattaaaat ccctaaatat atttaatata tattaaaaaa ttgtattata
     3481 atgaaaatca aaatagattg caaatccaaa aagtaatact taatgtccaa aaagctaaaa
     3541 ttaaaatcta aatagctatt tactacagaa gtatgttaaa atatgtaaaa tgaatagaaa
     3601 tttatatatt tttattaaaa atatataaga aaggatgttt aagaattgtg attaatatag
     3661 gaatatgtga tgatgaacta cattatcgaa taaaaataaa agatatatta agtgaaatat
     3721 taagttcata tcctataaat tataacatat atgaattttc agcaggagaa gagttactaa
     3781 ataactatcc taaagattta gacatattaa ttatggacat acaaatgaaa acaataaatg
     3841 gtatggatac tgctaggaag ataagagagt ttgatcataa gttagaaata atatttgtta
     3901 cttcatttgt agagtttatg caagaaggtt atgaggtaaa agcatacaga tatatactaa
     3961 agccaataaa taaagaaaaa atttctaaaa gtgtattacc ttgtataaat gagatgatga
     4021 aaaagagaaa taattactta actataaatg taaaaaatta tgtagaccga attaaaatag
     4081 attctattac ttatatagaa acagatagac cgaatatatt gatttataca catgatgata
     4141 tgtatattac taaaatgagt atatctaaaa tagaaaagat attaaatgaa tatggatttt
     4201 ttagatgtca taatagctat atagttaact taaaattagt agaatcaatg agtggaagta
     4261 ctgttatagt agatggaaaa agtattccaa taagtaaata tagggttaaa ggcttgaagc
     4321 ttgcgataac taatatatta ggggacatag tatgctaaca aatttaagtg taggattttg
     4381 gaatattatt aatttagttt caggtactat agattggatt atattttttt tagtaatcca
     4441 cttagttgga aagaggaaaa tatctaatat aagatatata tttacaacaa taattataat
     4501 tatatttatg gcatacataa atgtagttaa agtgtttcca aatattaaga ttcttctatg
     4561 tatggtaata ggaatatcat tttatataac atcatatgaa gataaaatct ataaaagtat
     4621 aatagttagt ttattgtttt ggctaggatt aatggttgct gaaggttctg cagtaggtct
     4681 agtagtattt ataaataaat taaataatat taacataaca caacatgaaa acttatttag
     4741 aatacaagct attattatgt caaaggtatt tttatttata ggttttatat tgtttaagta
     4801 ttttaaatta tctttagatt ttaaacctaa agatatga
//
LOCUS       DAYATN010000204         4805 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.208, whole genome shotgun sequence.
ACCESSION   DAYATN010000204 DAYATN010000000
VERSION     DAYATN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.208"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            134..1381
                     /locus_tag="ACGI8A_003543"
     CDS             134..1381
                     /locus_tag="ACGI8A_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFH0181507.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLTFGLSKM
                     QGVCILLLGAGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            1468..3462
                     /locus_tag="ACGI8A_003544"
     CDS             1468..3462
                     /locus_tag="ACGI8A_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0181508.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            3462..4784
                     /locus_tag="ACGI8A_003545"
     CDS             3462..4784
                     /locus_tag="ACGI8A_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFH0181509.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
BASE COUNT         1959 a          460 c          825 g         1561 t
ORIGIN      
        1 aggttttgaa aaaactatgt tcaatataat taaataaatt aaaatataat tgacaaagtg
       61 gatacagaat acttaaaata tatttaaata aaattaagca aaagatagtt aatttatgat
      121 aggaggggat attatgcaaa taacgacttt tggagctata tttggtttat taatagcaat
      181 tattttaatt ataaaaaaat ttcaagctgt atatagttta atgttaggag catttattgg
      241 aggattagtt ggaggagcaa atataacaca aactgtagat tttatggcaa atggagctat
      301 gaatatatca ccatcaattc ttagagcttt ggcatcaggt gtattagctg gaagtttaat
      361 aaaaacaggg gcagtagata aaatatcaga acagattgta aaaatatttg gtgaaaagag
      421 ggctttattt tcgattgcca tttcaactat ggtacttgct ggagtaggtg taaatttgga
      481 tgtatctata ataactgtag cacctatagg attatatata ggtagaaaat taaattattc
      541 taaattgtca atactattag ctatgttggg agggggtaaa gcaggtaata taatatcacc
      601 aaatccaaat actatagcag tagcagataa tttttctgtc aatctctcta gtgttatgat
      661 ggcaaatata attccagcaa ttattggagt tgttattaca gttattttag caagtatatt
      721 aataaataaa ggaaataaag tacaatcata tgaaatttta gagcaaagag aagatttacc
      781 aagtttattt aaatcattat gtggtccaat tatagctata tttctattgt ttttaggaaa
      841 tgtatctcct atagttatag acccaatgat agcccttcca ataggaggaa tcgtaactct
      901 aattgtcaca ggaaacttga acaattcaag agaatattta acttttggat tatctaagat
      961 gcaaggggta tgcatattat tacttggagc agggactatc gcaggaatta tacagatgtc
     1021 tgaattacaa cagagtacaa taggtgcttt acaattttta aatatgccac aatttttatt
     1081 ggcaccagtt tcgggtatac ttatgtcatt agcaacagca tcatcaacag ctggtgctac
     1141 tatagcatca tctacttttc atgatgctat aataaatggt gggctatcac cgatttcagg
     1201 tgcatcaata gtaaatgctg ggtctagtgt attcgagcaa ttaccacatg gctcactatt
     1261 tcatactagt gcaggtagta taaacatgga tataggtgaa agatttaagt taattccata
     1321 tgaagcattg ataggaatag taatgactat aatttcaaca tctattcaat tagttttata
     1381 ataaatataa atatttaatg aaaaaagtag gattttgtta ttaaatattg aacttattat
     1441 agtaaaaaag aagattggag ttttaaagtg aataaaaaaa agattgttat tatagggatt
     1501 atttattcat ttttagtagt attttcactt acaaatatgt atgtaaatat ggagtataat
     1561 ctaaatgtat ttgaatatat taagaagtca cttccattta cagaagaaga aaaaaagtgg
     1621 ttagaaaaac acaagaattt gatttacagc tctgaccaaa gttcacctcc tttgagatat
     1681 aaaggtaagg aagatggaca gtataaggga attattgttg atttaataaa ttctctgtca
     1741 attcaaattg gtagagattt ttattttaaa ccaaataatt ggtggaaaga atctttcgta
     1801 aattcaatag atgatagtat aaagtttttt gacttgattc catcaaaaga aagggcaaat
     1861 aaatttatat ttactgaccc aatatacact ttgagtgcaa atatattgaa agataaaaag
     1921 tcccaagata taaatagtta catggattta aaggggaaaa cagtagcaat tccagaaggt
     1981 gattattcta taaatttttt aaagcaaaaa attcaagata taaacatttt attgactcca
     2041 gatataaaga ctggagtcaa ccatttaatg tctggaaaag tagacgcagt tgttggtgat
     2101 gaaccagtat tgcgatacta tataaataat tatggacttt caaataaata cagtgtttta
     2161 agtaatccta tatatactaa aaaagcagtt cttgctgtac ctaaacaata tgaagagctg
     2221 gttagtattt taaataaagg catttttaaa cttcagaaaa atggagtgta taaagattta
     2281 aagaaaaaat ggtattctac gtataatgaa gttgatgata tattgtatga gcgaggaatt
     2341 gtgcctagta tttatctttt tataggtata attttaatat caatttatgt tttttatagc
     2401 tatacatatt tgttaaagat agaaatcaaa aggaaaacag aacaggtaat agaaaataaa
     2461 aaaactttag aggcaacttt taatagtatt acagatataa taatgcttgt agatgaaaat
     2521 aacaatatag tagaatcgaa caaagtacta tatgacttta tgggtgaaat gagttataag
     2581 atagcagatt tgattagcat gataaaaggt gttattgaaa ataccttctc tgaaaatact
     2641 aataaaacta gtgaaataga aatacacaat aaaattttaa agataaacac ttttcctgta
     2701 gagtataaaa aaaataatac tgagtatata gtagttctta ttaaagatat aactaatgat
     2761 aaaatagtag aggcgaaatt acttagagaa aacaaaatga tatctatagg gcaattagct
     2821 tctggggtag cacatgagat aagaaatcct ttgggtataa taagaaataa ttgctatctc
     2881 ttaaaagata acgttacaat ggaagaagta aatgattgtg ttaaatcaat agaaagtaat
     2941 gtagataggg caagtaatat aataaccaac ttgttaaatt tcgcaagaat atcagatgat
     3001 aatttagaac atataaatat aaaaaatttt attgaaaata tagtgaaact tcaatataaa
     3061 atgttacagt taaagaatgt tgaaataaaa attgactgtg aacataattt aatatgctat
     3121 ataaatggag aatcgttaaa acatgtattt ataaatttaa tttctaattc aatagatgca
     3181 atacatcaag atggaaaaat tattatatac tgttatgaaa aaaatcattg tttatttata
     3241 gattttaaag ataatggtga aggaataaaa gaagatgtcc taaaggacat atttaatccc
     3301 ttctatacaa caaaaccaat tggtgaggga actggtttag gtttatatat tacttataat
     3361 gagattaaaa agaataatgg agatataagt gttgaaagta aattgggggt tggaacttgt
     3421 tttcatataa aaattccatt aaataaagag gtgactatat aatgaaaata ctattagttg
     3481 atgatgagct agaatatgga gtggtaatga aaaaaattct acaaaagaag ggctatcttg
     3541 tagatatagc actaagtggt gaagaagcta taaatataat taaaaaagat aaaaattatg
     3601 atttggtttt atctgatgtt atgatgaaaa atatggatgg agtacagcta ttagatagaa
     3661 ttaaatctat taatagagat atagaggtta tattagttac aggatatgga agtattgaaa
     3721 atgcagttga tgctatgaaa agaggagctt tatcttattt tataaaaagt aatcccattg
     3781 aaaatttatt agaagaagtt gaaaaggtaa aggcaagtaa aatatctgtt tctgtacaaa
     3841 aaaataattt tgaatttact ttggaatcta aaaatagaga ttttaataat atcataaaaa
     3901 tagcaaaaaa agctgcttgt aaagatgtta atatattaat cttgggggaa tctggtgtag
     3961 gaaaagatat attagctaga tatatacata gcataagtcc aagaaagaat gagatatttg
     4021 tacctgtaaa ttgttgttct ttttcagaaa atctattgga gtctgagctg tttggtcatg
     4081 aaaaaggttc ttttacagga gcagtagata gtagaaaagg tagatttgaa ctttcgaata
     4141 aaggaactct atttttggat gaaataggag atatacctct aaatgttcag gtaaaacttt
     4201 tgaggacact tgaggacaaa tctatagaga ggataggaag taataaatct attaaagttg
     4261 actttaggtt aatttgtgct atgaataaag aaccaaaagt ggaaatatca aatggaaata
     4321 taagagaaga ttttttttat agaataagta caataacaat aactatacca ccactaagaa
     4381 agagaagaga agacttaact actcttatag aatttttctt aaataagtac caaattgaac
     4441 atgataaaaa gatacactcg attgataagg aagtaaagga ttttttatta aattacaatt
     4501 atccaggaaa tataagagaa ttaaaaaata taataaatag acttgtagtt ttaagtgaag
     4561 aaggtaattt atcaaaagac aatttaaatt taatatcaaa taatgtatac atagatgata
     4621 aaataagtat tagaccttta agagaaataa gaaaagagtt tgaatgtgag tatattgaaa
     4681 aagttttatc tttgtgtgga aataatattt caaatacagc taaaaaattg gagattagta
     4741 gaagacagtt aaccaataaa atatcagagt ataatattaa gtgagaaaaa aacttctcat
     4801 taagt
//
LOCUS       DAYATN010000205         4750 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.209, whole genome shotgun sequence.
ACCESSION   DAYATN010000205 DAYATN010000000
VERSION     DAYATN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.209"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..704)
                     /locus_tag="ACGI8A_003546"
     CDS             complement(<1..704)
                     /locus_tag="ACGI8A_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFH0181510.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIIT"
     gene            978..1652
                     /gene="fsa"
                     /locus_tag="ACGI8A_003547"
     CDS             978..1652
                     /gene="fsa"
                     /locus_tag="ACGI8A_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFH0181511.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVGKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1774..3168)
                     /locus_tag="ACGI8A_003548"
     CDS             complement(1774..3168)
                     /locus_tag="ACGI8A_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFH0181512.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3246..4592)
                     /locus_tag="ACGI8A_003549"
     CDS             complement(3246..4592)
                     /locus_tag="ACGI8A_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0181513.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         1841 a          666 c          619 g         1624 t
ORIGIN      
        1 tctgtaatta tagaactaat atcttctaaa tgagcaaact tgaaatttcc atcaaattta
       61 aactttttat cttccataac tataaatact tttttacttg aatttattat agccttcttc
      121 gtaagaccat cttcaatttc aaaagttgta agtgaagagt tgaatacatc tactccacaa
      181 cttcccataa aagtcttatc aaacttatat ttcgatataa actctatagt tggagtaccg
      241 acaaaaccgt ctaaactgag atttaataaa cctccagtgg aaattatatt taaattattt
      301 ggttttgaag taactacatt tagtatatcc agcatatttg taacaagtgt tactttttta
      361 ttactttcag ctaacaactt cgcaagcaag atgttaattg tagagatatc taaaaatata
      421 gtttctctat cttcaattaa attaaatgct ttttctgcta ttattttctt tgttggaata
      481 ttaatttctt ttctgtcttt tgtatcctga ttctctagtg tttctctagc tagtacagct
      541 cctccataaa ttcttcttat tgaagaaatc ttttctatat tttttaaatc ttttcttata
      601 caatcttctg taacattaaa tttagaactg agctctttga cagtaactct accttctttt
      661 tttaggatat ttaatatagc ttggattctt tcttctgcaa acattttcta cctcttttcg
      721 caatatagta ttatagtaaa tattataaga taaaatacaa tatatcaact attttaacat
      781 gaaatacaaa attaatttaa tataaaaagt atgttttttc taactatata tttagaattt
      841 agtaaataaa aaaattataa aactaagaag gaaaagtaaa acaataataa aaaataaaaa
      901 acaataagca aaatgttgaa aataatacca aatcaatata taataagaat aaagaaagat
      961 taaaggggga tattttaatg caactaatct tagatacagg taatgtggag gagattaagg
     1021 agttatgtac ttgtttacca atagatggtg taactacaaa tccaagtatt gtttcaaaag
     1081 aaaaaaagaa ttttaagcaa cttataaatg aaattggtga gattataggt gaggatatgc
     1141 ctatacatgc tcaagtgtta agtacaaagt atgaagaaat attagaagaa gcattatata
     1201 taagtagttt gagaaaaaat atatatgtaa aaatacctgt aacacaagat ggtttaagag
     1261 ctataaaaga ccttcacaga aaaggtgtaa aaataactgc tactgcaata ttcactgctc
     1321 atcaaggttt tttagctgca aaggctggag caaattatat tgcaccttat gtaaataggc
     1381 ttgataatat atctggtgat ggagtagcta tggtttcaga acttataaaa ataatagata
     1441 cttataaaat ggatacaaaa gtacttgcag ccagttttaa aaatgcacaa caagttatag
     1501 aattaatgca acatggggtg catagtgtta cagtaccata tgatatttgt aaatctatga
     1561 tgaatcatcc attaactgat tggagtgtag gtaagtttat agaggattgg gagaatactt
     1621 ttggaaaagg aagtaaaaca aataatattt agtattatgg taaaagagta gttttgtcca
     1681 atttcacaag aacttatata aaaagatgaa aatatgaatt ttctttttaa taatttcgtt
     1741 attatcttaa gttaattcat attttcatct tttttagatt tctagtccta taaatttcca
     1801 ccaagggaat gttatacata ttattataaa gtaggttaaa ggaatcaaag ttaaaccgta
     1861 tttgaaaata accttgctat tatacagatt ttcaccatga ccaaccatta ttgtagcatg
     1921 atgaaatggt aatatgtact gtatgcttac tataatgtag acaaatagac ataaagtaac
     1981 actattttcc tttagtatat gtaaacttcc aagtaacggt attactacag atgatgtagt
     2041 gacagcactt ccaagacata tattcaagat tattgtgaga gttatcaaaa acaatattga
     2101 aataatttct gaagagtttg gtataaactt tatcaaataa tttcccatta tatctacaac
     2161 tccagagtac tttaacactc ctccaattga aaatgctgct ataaaaaaaa taaggacctt
     2221 ccaatttatt tcttttaaag tactaaatcc aactattttt cttagtaata aaagaagtac
     2281 acaaattgac attataatta atgtgtttat gttatgtatg aactgagtta aaaacataaa
     2341 aaacattatt cctaggcata aaaatacgtg tttttgctct ttgtttagca aattaaattc
     2401 tttttttaaa ttagaatatg aaaaatgaag tttattaccc tcaagatttt cattagaaat
     2461 aaattttatt gaatgcaatt cttttttaaa gacaaagata aataaaaaat aagttataaa
     2521 acaagtaatt atagttggaa cagacatata aaaagcccat tgaccccaat ttatattaca
     2581 atttccaagt tctagtacaa cataatttag aagggtgtct ccatttacaa aaaacattga
     2641 tgtaaaggta gatgctgtaa atatactaaa taaaagtata gattttgaat cttcagttat
     2701 ctgttgttct tttaaaaact ccttgtaaaa agcagataca agaataaccc ttggaaatgg
     2761 ttgtgggata aaaaatatta aaagtatacc taaaatatac gataaaagaa tcattttttt
     2821 aggggtattt acagtattta aaattaactt ctcagaaata atttttgcga cacctgtttt
     2881 tgtaactgca ttagtaatta tgtatgcaag aattattatg taaaaatttt ctgtaaataa
     2941 aaactgtaaa ataaccttta ttggtgcaat gttaaatata aacgctagaa ttacaaaaaa
     3001 caatgcaaca aaagtttttt caactgagtc tacagcccat atcaagatag ttaaaaaaga
     3061 taagcatata gaaaatttta tgttgaaact gtaattaaga gagaagtaga gaattatggt
     3121 agctataaca gtcatataaa attgttttgg cttaagaata atacccattt ttatattcac
     3181 ctccataatt tattatatca taatggaggt gaatacaatg aatgtgaata cattttataa
     3241 aaagattatt cattgattct atgatttaca atagctttag ttttccaatg tcctttctta
     3301 tagtagataa ctgtaacaat aagacctaaa gtccaaccaa aaggaatacc catccaaatt
     3361 ccgtctgttc caaaaaattt agaaagcata taagaagctg gaagtcttcc aagccataaa
     3421 gatactattg agcttataag tgcaaacatg gaatcaccag cacctttcat tgcacttgaa
     3481 agtacaaaag aagttccaat aaataagaag aatggtccta ttataaataa gtaagaagag
     3541 ccaattttaa tgacatcttt atcagtattg aatagagcaa ttagattttc cctaaatagc
     3601 aaaattaaag caataactgt aactgctaaa actaaggtca tttttataga ttctcgaatg
     3661 ccttttttaa ctctttcatt ttctccagca cctatgtttt gagcaacaaa agtagaaaca
     3721 gcagaaccaa gattcataat aggtaagctt ataaaatttt ctatcctaag accagcacca
     3781 aaagctgcca tagcagatgt accataacta ttaactaaaa attgaagagc catatttcca
     3841 attgaaaaca acatttgttg aactccactt ggaagtccta atctaagtcc atctttaaat
     3901 attttgttat catatacaat tcctttgatt ttaaatttaa gcacttcatg ttttttattt
     3961 aaataaatta tacttattat aaatgatact gcctgagcca ttatagtggc aagtgcaacg
     4021 ccctcaacac ccattcttag cactacaata aaagttaagt ctaaaactat atttagaatt
     4081 gtggctatta tcagaaaata aagtggagtt actgaatctc caagtcctct aagtattgca
     4141 cttactgaat tatatccagc agaaaataat attccactaa atattatagt aagatacaat
     4201 tttgattgag caaaaacgga ttctggtgta ttcataagct taagcattgg gccaccaaat
     4261 actattccaa gaatacttat taatatagaa ccaataaata aaagtatata tgtagtatca
     4321 atagtagctt ttaccttttt taaatctcct gagccataaa attgtgacac taaaatagaa
     4381 aatccaaggc taacacccat aagcgctgcc accaataaaa acattatagg gttagctcca
     4441 gagactgccg ccattgcttc ttttccgata aatctaccaa ctattatact gtcagctgta
     4501 ttgtatagtt gttgaaataa actaccaata agcataggca tggcaaaaaa gaatatggac
     4561 tttccctcat gacctgtagt taaatctttc accttattcc tccaaataaa aaaattatta
     4621 atttattata gtataatctt aacataaact ttatatccaa actataaata aattgtatca
     4681 aaatatcata tattttttta aaatgtaaag aatataatta taatttgaat ttaataaatc
     4741 aatatttaat
//
LOCUS       DAYATN010000206         4697 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.210, whole genome shotgun sequence.
ACCESSION   DAYATN010000206 DAYATN010000000
VERSION     DAYATN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.210"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            40..1047
                     /locus_tag="ACGI8A_003550"
     CDS             40..1047
                     /locus_tag="ACGI8A_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HFH0181514.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            complement(1259..1957)
                     /gene="yunB"
                     /locus_tag="ACGI8A_003551"
     CDS             complement(1259..1957)
                     /gene="yunB"
                     /locus_tag="ACGI8A_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HFH0181515.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            2171..>4697
                     /locus_tag="ACGI8A_003552"
     CDS             2171..>4697
                     /locus_tag="ACGI8A_003552"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFH0181516.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGG"
BASE COUNT         1766 a          648 c          743 g         1540 t
ORIGIN      
        1 ttctattatt aaaataattt aataagaaag aggtgtttta tgcaacttaa aaattttcat
       61 tttaaaaata atactttaag taaaaaaacc aacttagcta agtcaaaatt tacaattgtt
      121 ttaacatttt tatttttatt atattttttc tcaaataatt taatttatgc acaaaattta
      181 caaaaaaatg aacctgaata tttaatacca attggaaacg tacttcaaat tgacgcagaa
      241 ttaaaaaata tcatagttag aaatcctgga gaagcatctc catttagatt gggtgatgct
      301 atagtaaagg taaataatgc tgaagttgat ggatatagtg actttgccaa taccttaaat
      361 gctttaccag aagaaaaaca agtatctatt ttattaaata gaggtggaca acttataaca
      421 atgaaaacta ccaaacacat attagaaaaa attagtttta acaatttact ttctggattt
      481 gctactttaa cgtatatcaa tccagataca tcagagtttg gagctgtagg tcatccaata
      541 agtgttggta atgcaagaaa aatacctatt aaaacaggtt caatttctac aactaagcac
      601 cttaatatag aaaaatctta taaaggtagt gttggatgta taaatgcaaa acgagaaaat
      661 attattggta gctttacaga aaatactgat tttggaataa gaggtcaaat taataacttt
      721 gatatttcca agttaaaaaa atataaagta gcatctttag atgaagttaa attaggaaaa
      781 gctcaaataa ttttacaaga taactttaat aagtgtaaaa aatataatat tgaaattata
      841 gatatagaaa accaaaaaca tccagaaagt aaaagtttta aaattaaaat tacagataaa
      901 aagcttttag ctcaaactgg tggtattgta caaggtatga gtggtactcc aatagttcaa
      961 gataataaaa taatcggagc agtgtcacat gcaattgaaa acgacccagc tgtaggttat
     1021 ggtgtattta ttaaatggat gttgtaaata atataaataa ataagtttta agatgttatt
     1081 aagttattaa taatatattt ggtctaaagg tacttttatt taacctacag accaaatata
     1141 ttagatattt tacatagtat aaaatataaa tttttattaa aattatatta atattatttc
     1201 caaaaatttt atttataaac tattattttg aatttattta aaaatataca actttacatt
     1261 acttgttggt attctttcca gtgttaggta aaacatttcc tagatttttt tctggaatat
     1321 ttacataact ttctggaaca tcacctacta ttatagtttc acaaataggt atttgagctt
     1381 taatttgttt tgttgaagtt ataagtggta ttactacttt aacttgtgtt tgagcttcta
     1441 aatatattct atgtctagtt tgatttatac ctgaagactc aaattccgtt ttaaagttta
     1501 ctgatactgt tcctataggt tcaatagata cttcaagttt aggaccgtat ttagctaata
     1561 taggacttcc taatgctgtt cctataggta tgtaagaact tgctgtctta tcctttttta
     1621 attcatcttg tatttctaaa gctattgcag atgcaatttc attcatcatt atagtgtttg
     1681 cttgtatcat agttatttta ccattattat caagttgtat atccattaaa tcttcataat
     1741 ttatttttcc ttctaccatc tcagcaacag ctttatttat cgacctattt gctagttcaa
     1801 gagctttagt ctctgctaaa actgttattg tcggtcttaa agttttatct atatatataa
     1861 aactgcctat aaatacagac aagaaaagaa ttactaaaaa aatagctatg atttttctaa
     1921 attcattttt tgtttttcta tctactattc tattcaaaat tatcccctcc tatttattta
     1981 tatgtagctt tggcataatt tttataatat ttgtcaaaaa taaataaaat aaaaatatag
     2041 actttttaat atattacgtt tatacatata caatgtacag aatattatag caaattcatt
     2101 ttttctatta attacaaaac tactcctatt tgtgttataa tattgcatta agtaaggagg
     2161 ttaaaatctt atgaataacg ataatgataa aaatggaaat aaaatcagga gaaaaaaagt
     2221 tagctcatct ggtaatacaa ataaaccaat taatagaact tcagctaaca agactcgttc
     2281 aacaaaaaac aagaaaaaat ctaaaaaaag cgataaattt aaaaaactaa gagtatttgg
     2341 gatagtattt ttagttttac tagttgttgg tacagcaggt actgctggtc tagtatttgc
     2401 gtcactaaga gatgtaactc cagttacaga agctgtttta gataaacaga cgaaccaaac
     2461 aactactatt aaatacgcta atggaaaaac attatctaca gctccaagtg taaataaaaa
     2521 gacacccgtt ccattagata agatttcccc ttatcttcaa catgctgtaa ttgcaataga
     2581 agatgagcgt ttttatgagc ataagggtgt cgatattaaa ggtttattta ggtcagtatt
     2641 aaagaccctt acaggtacta agcaaggtgg tagtactatt cctatgcagg tatccaaaat
     2701 gctgttaaca actgaacagc aaactatacc tcgtaaaata aaggatattt actatgcaca
     2761 tgagatgagt aaaacagtaa gtaaagataa aatactagaa acttatttaa ataacttctt
     2821 cgttggtaga ggtctagcag gtgctgaggc tggtgcacgt ggttattttg ataagtctgc
     2881 agctgacctt actctagcag agtcagcact tcttgctggt tcaactaaaa atccatcaag
     2941 attttcagcc tataaaactt ctaagttaga gggaaatgaa actaaggagg atttagagaa
     3001 taaattatta ttttttgtta acacaactga tgatgattta gatgacccta ctcaagtgga
     3061 ctttgatatg attgaaaaaa taaaatcttg ggagcttata tctaatgata cttatagaca
     3121 attaaaagca ggtacattag ttgttagaaa ggctgtaagt aatcctgaag ctaaaaaaag
     3181 acaagaaatt gttttaaaga aaatgttaga attgaaatat ataaaacaaa gtgaatatga
     3241 tgaagcaatc aaagctaaaa tagaaataaa attgcctcaa tcatcagata aagtttcttc
     3301 atctgtagaa gaccttatcg aaagtgaagt tataaatgct ttaatggaac aaggacatac
     3361 aaatgatgaa gctcaaaact tattctacaa tggtggtcta attgtaaaca caactataga
     3421 ccctaaaatg caagatgctt tagaagaaga atttgataga aatagcaatt tcccaggaca
     3481 tatggtcggt cctgatggag ttagccaacc gcaagctgca atggtaatct tagattataa
     3541 aaatggtgaa ataagagcac tagctggagg aagaaatata agtggtagaa aaactttaaa
     3601 tcgtgctact aaccctcatc agccaggttc ttctataaaa cctttagcaa tatatacgcc
     3661 agctattgat actctaaaaa taacacaagc aactgctcta agtgattctc gaggaggata
     3721 taagtttgaa gaaaataata aatggaatcc aagaactact actgctggtc atggttctat
     3781 gagtttacgt aaagcacttg ctaaatcttc aaacacgata gctgtcaaaa ctgctgaaat
     3841 gcttggagac tcttatgatg aatgtgtaga tataatgatg gattacttaa agaactttgg
     3901 tataacaact ttaaagaata atcactcagg ttcatctgaa gcaagtgata gaaaattccc
     3961 atcattgaca ctaggtggta tggcgaatgg tattactcca cttcaaatgg ctgctgctta
     4021 tggtacattg gcaaatcacg gtatttatgt agaaccttct atttttacta ctataactac
     4081 ctttgatggt caattattag ttaaaaatgc tcctgaagaa cataaagttg tagaccctga
     4141 agtagcttat gttgttacag atatgttaga aagcgtaatt actgaaggta ctggtggtgt
     4201 tgctacactt ccaaagggaa tgcctgtagc aggtaaaaca ggtactacaa atagtgcata
     4261 tgatgcttgg tttgtcggtt acactcctta ttatgttggt gcaacttata ttggagatga
     4321 tgctggtaga aaagatgatt ctggtaacac tataaaacgt agagaagtac cacatggaag
     4381 tactagtact gcaaaactat gggaaaaaat tatggaaaaa atacatgcta atttaacggt
     4441 tactgaattt gaagttccta aaaatgtata ttttacaaaa attaatttag aggatggagg
     4501 aaagcaatct tctggctcta aagcagcatt tattgaaggt actgctccta ctaaagtaag
     4561 ttctcaacca tcatctgaag atacaaaaaa accagacaat aaccaaccag aagaaaataa
     4621 taataacaat aataacacag gtgataatgg tggcggttct acacctcctg ataatggcgg
     4681 aaacaatggt ggtggct
//
LOCUS       DAYATN010000207         4663 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.211, whole genome shotgun sequence.
ACCESSION   DAYATN010000207 DAYATN010000000
VERSION     DAYATN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.211"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..297
                     /locus_tag="ACGI8A_003553"
     CDS             <1..297
                     /locus_tag="ACGI8A_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFH0181517.1"
                     /translation="NSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDI
                     KLIDKRIQTSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            309..449
                     /locus_tag="ACGI8A_003554"
     CDS             309..449
                     /locus_tag="ACGI8A_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HFH0181518.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            508..1089
                     /locus_tag="ACGI8A_003555"
     CDS             508..1089
                     /locus_tag="ACGI8A_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HFH0181519.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            1236..2945
                     /locus_tag="ACGI8A_003556"
     CDS             1236..2945
                     /locus_tag="ACGI8A_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181520.1"
                     /translation="MSSIKAISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            2960..3832
                     /locus_tag="ACGI8A_003557"
     CDS             2960..3832
                     /locus_tag="ACGI8A_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0181521.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLIDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            3833..4621
                     /locus_tag="ACGI8A_003558"
     CDS             3833..4621
                     /locus_tag="ACGI8A_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181522.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVSYIARKSTVLKKSK"
BASE COUNT         1922 a          408 c          690 g         1643 t
ORIGIN      
        1 aattcactag ataatgcaat tgaagcatgt atggatataa ataatgagat agaaaaaaga
       61 atagaagtta aagctacata tataaataaa tttgcaataa taaaatttat aaatactaag
      121 gtaaatgata ttaaacttat tgataaacgt attcaaacta gtaaggatga tgataagata
      181 catggaatag gacttgctag tattaaatat atagtaaata aatatggtgg tgaaacaata
      241 gttaattact cagataatga gtttatatta aaaataatga taccaataaa aagttaggag
      301 agtacaaaat gaaaaaaata gcgttaaact tacttaaaaa catctcagca ttaagttttg
      361 ggatagctgt attatcagca aacagtgcaa gttcttgggt agcacatcag gcaaaagagc
      421 cacaagcttt acaaaaacta aaaaaataac attaataaaa ggacctcttc ttattaaagg
      481 agaggtttta catgttgagg aggaaatatg tttaaaagat tatcatataa atttgccaat
      541 atattagtta ataatgagat tgtagaaagt gaagattttg aaatatatag atatggattt
      601 gaaactttga tttatttcat agttaatatt ttggtagcat tatttatagg aattattttt
      661 gatagattta tacatacagt tatattttta agttgttatt gcactttaag acagttcaca
      721 ggaggttatc atgctaggaa ttataaagag tgtacattaa catttgcagt tatttatttg
      781 ataactattt tttcagctaa taatatagat ataaataaat ataagtattt attagtatta
      841 ttaatgatta ttagtatact aacaatttac aagttagctc ctttagaaca tagaaacaaa
      901 cctcttagtg aaagtgagaa aaagcattat agaaagactg tgcaaaaaat actattcgta
      961 ataatatgtt taattatttt atgtaaaata cttaatattt ttcagcaata tgtgatttat
     1021 gcaataattt ctatatattg gattgctata ttaatatata ttggaatgaa ggttaatagt
     1081 gatcagtaag attataaaac attttctaaa ttaattaact tcactaatta atattaactt
     1141 taaaaaaata atgtcaaggt aagtatttga acttattaaa tatagttgaa tctattgtag
     1201 gttgtatcaa aagactggct aggaggggca taaatatgag tagtattaag gctatatcag
     1261 actttataac caaaataatt agaaaaaata ttttatttgc aataacaatt attattataa
     1321 attaccatct catacaacga tggacacata tatccaatag aggaatttca tttaatgatt
     1381 acttatcctt gagtgtatat acttgtatga caattagtat tttaaccatg ttaaatgtaa
     1441 ttttttatat gagaaaaagt tatgatactg taaaaatatt atgtagaaat aattttgatt
     1501 attttttagg tatttctgtt gctgtaacta gaaacaactt tatattttgt tgcataccaa
     1561 ttatatatgc tatagcatac tcaagtttaa taagaacatt gtcaattgta acgattaaat
     1621 caactatatt tatattgata tttgaatggt ttttaccact tactatttta tctatagtga
     1681 tatcatttat ttcagttatt ataaataatt taatactaaa gattacctta tcatcagtat
     1741 ttttatattt aacatcaact aatatgttgg atagagttat tgatattaaa gaaccatttt
     1801 ttaaagcatt atttaaatca cttaacatat ttgaagatca atcatatgct tactattctg
     1861 attttgtagg gaaagttaat aattcagcat atgttataga taagattata cctacactat
     1921 ttgcaattac tttaataata gtagcatatc tgatattaaa tgatattaga aagaaacaga
     1981 ttaaatcatt aatagttata gtagtgtaca gtattttatt tattggatta aattatatat
     2041 caatagatat gtcaacatat agagatgagg agcgtagtta tggagagtat ttaaagaaat
     2101 ctaatattag tgatattaaa ataaaatcgc atgatatgaa gattgacttt aaaagcaagt
     2161 caaatataaa agatagaatt gttttattaa acacatcaaa tgaagagtta acaaatatta
     2221 atatgatttt agatgaaata tttgatataa aatatataaa aataaattcg gaaaatataa
     2281 aatttaacaa aaaaaaggat ttaattgaag ttaatttaaa ggaaagttta gagcctaatg
     2341 acgaggtgac attagatata gagtatgaag gctatattaa tgtgttgaca tcttggaatc
     2401 aaaataaata tatagctact aaagatgata ttatgcttcc accaggttct ttagcgtggt
     2461 acccaaaagt aaatcaatca agttgtatgg cttataaaat aaatttaagt tctaaaaata
     2521 atgtctattc aaatttacct ttactgaata agactagtaa ttttttaaaa aaagattata
     2581 tatttgaagg aaagtcaaat gatattgcaa tatatgcagg tgagtttaaa gaagaggtaa
     2641 aagatgatat aaaaataatc tatccatcag atagtaatgt aaatatggat atagaggaaa
     2701 aaattaaatt tttaattgaa acaagaaatg aaatattagg tgatagcaaa gcatctgtag
     2761 atgctaaaaa atatgtagat gaagtagata atgttatgga atcaataaaa agaaaaaaaa
     2821 ttaatatgat attagttgct gacttaccta gcaatgaatt tttatatgaa gactcaaagg
     2881 gagagaaaat tagcataact atttatcatt ggctttcagg aaatatcctt ataatagata
     2941 aataataaag gaggttaata tgataaacaa gttagaaata agaaatctaa attttaatta
     3001 caaaaataaa aaagctttgg ataatataaa tttaacccta atagatggag tagttgcatt
     3061 attaggtcct aatggtgctg gaaaatcaac attaatgagg ttattagtga ctctttatga
     3121 aacttcaatt ggagagatag aattaaataa tataaagtat agcaaaaaca atgagaaaat
     3181 caaggcaaat gtaggatatg tacctcaaga ttttgatatg tataataata taaatgggca
     3241 agaatactta gaatttgtag ctaaaatgag aggtgtatct aagaatgatt taaagaaaca
     3301 tatacaaaaa gtagtatcaa aagttaatct ggacaagttt ataaataaaa aaataggtac
     3361 atactcaggt ggtgtaaaga gacgactagg tatagcacag gctttaatag gtgattctaa
     3421 gttaatagtt atggacgaac ctacagttgg tcttgatcca gagcaaagga atgaatttag
     3481 aagattgtta ccagaaataa gtaaaaattc tatagtatta atttcaactc acatagtaga
     3541 agacatacaa tttaactgtg acaaattaat tatattaaat caaggcaaaa tattatatga
     3601 tggtacaatt aataagttta ttgatatggt tgaggtgtat tcggtagtag tttctaatga
     3661 tgaatttgaa tatttagaac gtaatattga tattattgat tataggagag ttaaagatgg
     3721 tgtgcaagta aagtatatag gtaaaaatac agagaaactt aaagatagct taaggataga
     3781 tacaacttta caagatgctt atataaactt tttacataag gagttaaaat aaatgtgtat
     3841 attggagtta ataaaatttg agcttaaaaa aatagagctt aaacatgtgt taatatttgt
     3901 aattatttct atcttatgca tatttattac aggcatatta gattataatg agttaatatt
     3961 ttataatgat gtacctataa tggatacata ttctatagca ggatggttag gaattataaa
     4021 gatatcagat gtttttatac caataatgtc agcaatcaca ataatttatg tgttttcaaa
     4081 agattattcg ggtaatataa atgagatact tactttatat aataaaaaaa aatataatca
     4141 atttatagta tttagatggt gtgttatatt agctatatat actgtattat tattaatttc
     4201 aattacaata gtatatacat ttacgtcagt gaaaggtttt aacatagaca aaatagattt
     4261 tgtttatagt gtaaagccaa tagatgtgtt tttaaagaca tttcctacat tattatggta
     4321 tacaacattt ccactaatga tattaacatt aacaaaaaat agatttacaa caatagcaat
     4381 attaattaca tatacatttg ctgatatatt ttttatttta catatgtacc catttgttag
     4441 tatgattaat gtaaatggat tttatattca aaagatgttt tatatgccta acaatataga
     4501 tgttagattt aatgaattaa agaattattt ctttataaat agaggttctc tagtagttat
     4561 ttctataata atagtttctt atatagcaag aaaatcaacg gttttaaaaa agagtaaata
     4621 gtttaatata ttttttataa tgttatattt atatagaata gtg
//
LOCUS       DAYATN010000208         4559 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.212, whole genome shotgun sequence.
ACCESSION   DAYATN010000208 DAYATN010000000
VERSION     DAYATN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.212"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     misc_binding    149..383
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            433..1452
                     /gene="pheS"
                     /locus_tag="ACGI8A_003559"
     CDS             433..1452
                     /gene="pheS"
                     /locus_tag="ACGI8A_003559"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFH0181523.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1469..3862
                     /gene="pheT"
                     /locus_tag="ACGI8A_003560"
     CDS             1469..3862
                     /gene="pheT"
                     /locus_tag="ACGI8A_003560"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFH0181524.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3887..4477
                     /gene="zapA"
                     /locus_tag="ACGI8A_003561"
     CDS             3887..4477
                     /gene="zapA"
                     /locus_tag="ACGI8A_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFH0181525.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1808 a          522 c          925 g         1304 t
ORIGIN      
        1 tattatataa aaaataattg agttaattat gcattagata tatattatat aaaaaataat
       61 taaattaatt atgtattgga tatatattaa aaatagtcac ataatttgaa tggaaatgat
      121 ataatactaa aataaacaat ataatattgt aaatgcaatg aaagaggaaa gtattttgat
      181 taaacttagt aaagagataa acacctaggc tgggagtgtt ttctaagagg tcatgagaag
      241 ttccctctgg agtaacagag ctgaaatttt acagtaggct ttgacgtcaa aaacgcgtta
      301 agtttgttag aggtggtttg atgattttta aattgttaaa ctactagggt ggtaccgcga
      361 aactatagcc tttcgtccct agacagggat tgaggggctt tttttataca aaaaaacgaa
      421 agggtgatat gtgtgcaaga aaaattactt gctttacgtg aagcagcttt ggctgaaata
      481 aaagaagcac aaagcataga aagtgtagaa agtttaagag ttaagtactt aggaaaaaaa
      541 ggtgagataa ctgccatact taaagaaatg ggtaaattat ctgctgaaga aagaccagta
      601 gttggtaagg ttgccaatga ggtaagagaa aacattgaac ttagcataaa ttctaaaaaa
      661 gaagaaataa atgctattga aaaagaaaga aaattaaaag aggaagtgat agatgttact
      721 caaccaggaa aagttttaaa ggttgggaag aagcatccaa taactcaaat tatagatgaa
      781 gtaacagata tatttatcgg aatgggattc tctatagcag aagggccaga agttgagact
      841 gttgaaaaca actttgacgc attaaacgct cctaaagacc atccatcaag agatatgagc
      901 gatacattct atatcaatga tggggtatta cttagaactc aaacatctcc agttcaagta
      961 agaactatga gaagtcaaga gttaccaata aaagtaattg caccaggtag atgttttagg
     1021 tcagactcgc cagatgctac acactcacca atgttccatc agatagaagg gcttgttgtt
     1081 ggaaaagatg ttactatggc agaatttaaa ggaactatgg atatcttcgt tgaaaaattg
     1141 tttggttctg atatcaaaac taagtttaga cctcacaact tcccatttac agaaccaagt
     1201 gcagaggttg atgttacttg tttcaaatgt ggtggtaaag gttgcccaat gtgtaaatat
     1261 gaaggttgga tagaaatatt aggttcaggt atggttcatc caaatgtgct tagaaattgt
     1321 ggaatagacc cagaagttta cagtggattt gcatttggag ttggggttga aagacttgca
     1381 atgcttaaat acgaaataga tgatattaga ttattattcg aaaatgatat gagattctta
     1441 aatcaatttt aattaggagg gtatgtagat gttagtatct ttaaaatggc ttagagacta
     1501 tgttgatata gacatggatg taaaagagtt cgctgataaa atgacaatga caggaactaa
     1561 agttgaaaca atagattatt atggtgaaga aatagaaaat atattggttg gaaagatttt
     1621 agaaataaaa caacatccaa atgctgataa gttggttgta actaaagtag atattggaga
     1681 taaagttgtt caaatagtta caggagctac aaatatatca gaaggagatt atattccagt
     1741 agctgtaaat ggttctaagt tacctggagg agttgaaatc aaacagactg atttcagagg
     1801 tgaattatca gatggtatga tgtgttcagc agctgaacta ggtatagatg aacattacat
     1861 tgaggagtat aaaagaggtg gtatatatat tttagaccac gaagattctt atgaattagg
     1921 aaaagatata aaagatgttt taggattaaa agatgcttta atagattttg aattaacttc
     1981 aaacagacct gattgtaaat gcatgatggg tatagctaga gaagcagctg caactatagg
     2041 aacaaaagta aaatatcctg aaatcgaagt aaaagaaagt gacgaagaga tagatttcaa
     2101 agttgagata gataatccag atttatgtag aagatatgtt gctagaatgg ttacagatgt
     2161 aaaaatagaa ccttctccat attggatgca aagaagactt acagaagcag gagtaagacc
     2221 tataagtaac atagtcgata taacaaactt cgtaatgtta gagcttggtc aaccacttca
     2281 tgcttttgat ataaatcaag tagagactgg aagaatagta gtaagaaatg ctaaagatgg
     2341 agagaaactt gtaacattag atgatgttga gagaacatta gacaaagata tgctagttat
     2401 aacaaatgga gaaaaatcac ttggtttagc tggtgtaatg ggtggtgcta actcagaaat
     2461 aacttctaat acgaagactg tactttttga aagtgccaat ttcaaaccag aaaacataag
     2521 aatgacagct aaaaaagttg gtattaggtc agaagcatct tcaagaaatg aaaaagactt
     2581 agaccctaat cttgcagaga tagcagcaaa tagagctgca caacttgttg aaatgttagg
     2641 agcaggaaaa gttttaaaag gtgttgtaga tgtatatcca aataaaccag aacctaaaaa
     2701 attggtagta aatcctcaaa gaattaacca cctattaggt gtagatgtac caatggagca
     2761 gtttgtagga attttagaat cattagagtt taaatgtaat ttggtagcta atgataaatt
     2821 agaaatagat gtaccaagct ttagaacaga tatggaacaa gaagctgatg tatgggaaga
     2881 aatagctaga atttatggat ttgagaatat accttctgta caattagaag gaaatacgac
     2941 agcaggaatt aaaacttcaa aacaaaaatt catggatgct ttaaaagata attcaactgc
     3001 tgtaggatta aatgaaatat taacatattc atttgtaagc cctaagggag tagataagat
     3061 aagagtacca gaaggtaatg caaagagaaa ttttgttaaa ttacttaacc cattaggaga
     3121 agaaacttct gtaatgagaa ctactttaat accaaatatg ttagatgttt tatcaactaa
     3181 tgtatctcat aaaatagaag aagtatctgc atttgagtgt ggacatatat tcatcccaca
     3241 agattcagaa ttacctaagg aagaaaatag aatgtgtgta ggtatgtatg gtaaagatgt
     3301 tgacttcttt gcactaaaag gaactataga aactatactt gtaaatgtcg gatttaaagg
     3361 ttatgaaatt gaacctcaag ataataatac taccttccac cctggtagat gtgctaaaat
     3421 agtgtataat aataaatatg taggtacatt aggtgaattg catccagatg ttatagaaaa
     3481 ctacaatcta ggtcaaagag tttatgtcgc tgaaatagat attgactttg tatttgataa
     3541 ttctgataga acaaagaatt atgtgccatt accaaaatat ccatctacat caagagatat
     3601 agctcttatt gttaaagatg atgtatttgt aaaacaaata gaagatataa taaaggaaaa
     3661 tggtcaagga ttagtagaaa gctacaagtt atttgatgtt tacaaaggtt ctcaaataga
     3721 agctggatac aagtcaattg catattcaat aacttataga agtaaagata aaactctaac
     3781 agatgaagat gtagctaagg tgcatgataa gatattaagt gaattaagcg aaaaattaaa
     3841 tgctaactta agatcaaatt aacaactaat gtcgggggag gttaggatga acaaagtaat
     3901 ggttaaaatc catggcgctg aatatccaat ggttggagac aaatcagaga agtttatgat
     3961 tagtattgct gattttgtag acaaagaaat ggataaaata acacgtcaaa acccgaaatt
     4021 gagcttatca gttgctgcga tattaactgc attaaatata tcagaccttt tatttgaatg
     4081 ttcagatgag aatgagaagt tgattaaagc gaatgaagaa ttgagtaaga aggttggagc
     4141 atctaatgaa gagcttcaat tagaaatcaa atctttaaag cttacgatag cagaaaaaga
     4201 agcagaaaat cgtgaagctg aaactaagat gaaagagtta attgagatta tagaaaataa
     4261 aaagcaagaa atattcgaac ttagtaatac gacagaaggt tctagagcag agttagatgc
     4321 ttataaaaac aagattgagg agttaagtac tcaacttgaa gaagcaaatg aaagagctac
     4381 tatagctgaa aacttagctt ctgagtttca aaacaaagct tatgatttac agttaaaatg
     4441 tacagggtta aataatgatg caaaaaatgt ggaataaact aaaagatttg ctgtctcttg
     4501 ttttttgaga cagcttcttt taatgtataa atataaatgc tatataaata tagataatg
//
LOCUS       DAYATN010000209         4475 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.213, whole genome shotgun sequence.
ACCESSION   DAYATN010000209 DAYATN010000000
VERSION     DAYATN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.213"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            641..1321
                     /locus_tag="ACGI8A_003562"
     CDS             641..1321
                     /locus_tag="ACGI8A_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0181526.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
     gene            1769..2053
                     /locus_tag="ACGI8A_003563"
     CDS             1769..2053
                     /locus_tag="ACGI8A_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181527.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(2824..3039)
                     /locus_tag="ACGI8A_003564"
     CDS             complement(2824..3039)
                     /locus_tag="ACGI8A_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0181528.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(3050..3517)
                     /locus_tag="ACGI8A_003565"
     CDS             complement(3050..3517)
                     /locus_tag="ACGI8A_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFH0181529.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            3861..3959
                     /locus_tag="ACGI8A_003566"
     CDS             3861..3959
                     /locus_tag="ACGI8A_003566"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181530.1"
                     /translation="MFILKRLCKLVSINKSFSEEIPKNLKNTYMCI"
BASE COUNT         1877 a          375 c          555 g         1668 t
ORIGIN      
        1 aaaaaacata aatttataat ataaaattgt tttaagctcc ctttccccaa aataggaaat
       61 ttttattagc atttaataat gatttttaat ttttaatata atattaaaga attatttaag
      121 aatatttaat ctctaaagct taaattacaa taatattata aaaataaaag tgatatattg
      181 tgtaataatc gaaattttat actttggtta aaataatttt atatatagtg cctaatgtat
      241 aacctaatgc tcgtagacaa taatgtggat attatcaaat ttatttttat aatagaaatt
      301 ttaatttata tctatattaa attgttaaag atataattat gagaataaaa aatatagctt
      361 tgaattatat aattgtatat gcttatcttt atacagtaaa taaaaaatca aatgaaatac
      421 caaataaagt gtatatctta aaataactta ctagagatac aaggatgcta tgtgaatttt
      481 gaagtgtgca gtttagataa taaatatata taaataataa agaaaatgga tttatagtat
      541 ttaaaatttc ttataagggt aaaaaatgct attagaaaat gtactaaaaa tatttttcca
      601 attctttata gaaaaataat gatatttagg agagattgta atgcaaattg atgtattgat
      661 aattgatgga gataagaata gttctcaaga gctgaaaaat tttttagaag gaaaggggat
      721 aaatgtctat ttagcatata attgtaaaga tgctattata aaaatattta gcaagaaatt
      781 tgacttaata tttttagaga taattttagt tgatggtgat ggttgggatt tttgtaaaaa
      841 aattagaaat ataactactt gcccaattat tcatataact tatataaatg aaaatcaaag
      901 tattttaaat gctttaaatt ctggaggaga tgattattta ataaagcctt taaatttaga
      961 aatattatat gcgaaagtta aagctatttt aagaagaaga gatttatttt taaataataa
     1021 taaaaataat ataaaactag aagattataa tagagctagt ggagtaataa agttagaaaa
     1081 taaaatgata aagttaactc caacagaaaa tagattatta aattacttta tagagaatgc
     1141 tgaaaagact ctaactatta aggagatata tgaacatgta tggatgaatg agtatttaga
     1201 tgataattat agtgtagtgg ttgctgtgag tggattaaga aagaaaatag agaaagatta
     1261 taaaaatcct caaaaaatta taacgataag agaagtaggg tattacttta acaagatata
     1321 aagctttata aacagaatga atgataaggc atagataata tgttagtata ttataagagt
     1381 ttttgttcta acgatatatt tgtaaaatta gatttaattt acttaattaa attatagcag
     1441 tagaataaat ataaaaaatg tctgaaatat tatagacatc agtaaaatat ataaaactat
     1501 aatataagtc gtaataataa aacaaatgaa tattaattaa taaaaagata gtagctattt
     1561 ttatatagct attatttttt tattgttgtg gatgaaaata tacttatatt ttactaatat
     1621 acatttaaaa attataattt aaagcattaa aaaataaatt tgtattttgt tttatataaa
     1681 gtaatttgaa gaatatggaa aaacttgatt agtacattat taattagggt ataaaatggt
     1741 gtaaatagaa tgataagggg tggatgaaat gcttgtatat aataaaagtt tttatcctaa
     1801 tgacatattt ccaagattag atttttcaaa aataaaaaaa cagttaaaat tgatagataa
     1861 tgacctgtca gattttggaa gcatatgtat aatagaaaaa gaacattata caataagtgt
     1921 aaacagtata ggtgaaataa atgtgtatta tgatttagag tacgaaaata aggtgtatag
     1981 aatagtttat gagattgaaa agttatttaa atctcaagtt ggaaggttta gcatatctac
     2041 atacagaaat tgataattaa aaaagtagaa attaaaaaac ttaatactaa agatatagat
     2101 aaatatatga ggtaacagct agagagagtt gttacccttt ttaaatacta agtatatatt
     2161 attcttatta attttttgta aattcactaa aagacctttt tatagaatat tattttcttt
     2221 ttttattaaa taggtaaatc agtaaaaatg caattccagt tccaattatt attccaatga
     2281 tattttctat aattaatttt tttaatataa aagtaaataa taaaaatgct atacataaac
     2341 ctaaaatata tcctttaaaa taggttgaaa gttttctttt catataatca ccaacttgta
     2401 tcagatttag tttacttaat tagagtataa cagtgttgta acttcaaaga gtatctaaaa
     2461 gattacaaat gttagataaa atcatcaata atgtaattta taacacgtag ctataaacta
     2521 tgcaataata taaatggagg tataagtaaa tgaaaaagtt ccatgttttg ctaacagttt
     2581 aaataaatat taggtagaaa agaatgtata taggaatctg tggtaaaatt atcaatttaa
     2641 aagtattatt tagtgtgata cagttcttat aggtaatgga taacaaatag ataaaggaac
     2701 agtttctttc ttagaacata ttttgttatg actttaaaca aagttaatat taatgtcaat
     2761 aatatagtaa aagaataaaa atcatatagt ataataaaaa agagtgtttc taaacactct
     2821 tttttaatca ttttcatctt ctacatattc aagtatatca cctggctgac agtctaaagc
     2881 tttgcaaatt tcgtttagag tggtaaatct aatagctttt gcttttccag tttttaaaat
     2941 tgataaattt gcatttgtaa tatctatttt ttctgccaaa tctttaagtg atattttcct
     3001 ttttgccatc attacatcta ggttaactac tattgccata acatcacctc tatatcgtta
     3061 aatcattatc ttcttttatt tttatagctt tatcaaatgt ttctgcaatt acaaagcaca
     3121 ttagtgcaaa tataaaagag ataatcataa gtggtgagat accgccacta tcagttccaa
     3181 aaattcttat attattagta tttaaattat aaccattgat acattcaatt atagtgttta
     3241 tcaataaaca aaatcccatt accttaaacc tttttacatt ttcaagtacg aatggggaat
     3301 acttagtact attaattata aatatcaaga ataagatgac tataaagtag tcagaaaaat
     3361 atagtattct tgcaattaaa gcgattactt tgtcagaact aaaatcatat aaaaacatag
     3421 gtatcataca taagaatatt ataccagcaa ctcctaaaag tgataataga aaatatagaa
     3481 atactgataa tatattacta aaaaattttg taatcataaa atatcatctc ctttatataa
     3541 agtatattat tgattataat atacaattta ttgaatttca ataataaatt attgatataa
     3601 tatataaaac cattgtaata tacactaaaa ataagaatgt ataaggtggt aaatttatat
     3661 aaaaaagaat atgagttgta aaatattaaa taatattgta ctaattggaa taattttaac
     3721 tttattatag attattttaa taccattagt actgatggtg tattttaaaa ttggtataag
     3781 cattacaaat acgaatatgc caactgtaat atgtaatata agtagctata tacatatgtg
     3841 ctgtcccata tattgtatca ttgtttatat taaaaagatt atgtaaattg gtatcaatca
     3901 ataaatcatt ttcagaagaa atacctaaaa acttaaaaaa tacctatatg tgtatttagt
     3961 tagatttgag atttttacat ttaattttat agtgacaata ttatattatt tttatgatgt
     4021 ctatttatat gcaattacta taatatcaga acttatagta tcatttgttt cattatcaat
     4081 aaggtttttt actttagttt tgtcaatttt attaaaaaaa gttattgata taaaagataa
     4141 gaaggataaa actatatgac ggatttcaaa cataagagtg gatatttata taatagatat
     4201 tttatagtta attttattag attttattta atactgtaat attaatatca caatatatta
     4261 atagtgtatt aaagattgaa ttttgtagat aactataatt tattgagagt gataaataaa
     4321 aattatataa tattacagaa gaaagagagg gagacaatat tatatccctc ttttttctat
     4381 aataactaat tattaaaaaa ttattaatac tctatatact tttaatttct ttaataataa
     4441 ctaatttatt ctttatgtag ttattttaat aatgc
//
LOCUS       DAYATN010000210         4407 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.214, whole genome shotgun sequence.
ACCESSION   DAYATN010000210 DAYATN010000000
VERSION     DAYATN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.214"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            49..717
                     /locus_tag="ACGI8A_003567"
     CDS             49..717
                     /locus_tag="ACGI8A_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFH0181531.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            876..1382
                     /locus_tag="ACGI8A_003568"
     CDS             876..1382
                     /locus_tag="ACGI8A_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFH0181532.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1510..2526
                     /locus_tag="ACGI8A_003569"
     CDS             1510..2526
                     /locus_tag="ACGI8A_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFH0181533.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTPANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     regulatory      2722..2816
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            2880..3584
                     /locus_tag="ACGI8A_003570"
     CDS             2880..3584
                     /locus_tag="ACGI8A_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HFH0181534.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            3879..4358
                     /locus_tag="ACGI8A_003571"
     CDS             3879..4358
                     /locus_tag="ACGI8A_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFH0181535.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         1842 a          371 c          759 g         1435 t
ORIGIN      
        1 tatattatat taggtatttt aaataaattt tttaaagggt gattttttat ggaaaatata
       61 actagttatt ttacaacaat actatgtgta tttatatgct tatcatctgt atttatattt
      121 actcaactag ctagagtatt tattaataag aagaagatta atcaaaaaat taaatcaaga
      181 aacggattta ggtatgatag agattttatt gaagccagaa gagaagaaat acatataaag
      241 gataacaata ataataaaaa taagagtaac aataagaaat taaagggaga aaaagtattt
      301 aaatacgata atggtgattt atataaaggt gagtttgttg atggaaagaa aaatggattt
      361 gggatatata ttttttcaag taaggaaaag tatgaaggat tatggaaaga tgacaagatg
      421 catggaatag gaaaatatac ttatagagat ggttcaatat atacaggtga atttaaatac
      481 ggattaaaaa atggtttggg aaaattaaca tatcccaata atgacattta taagggatat
      541 tttttagata ataagaaaaa tggtaagggt gtattatata aaaatgatgg aaataaacaa
      601 gcaggaatat gggaagatga tgaacaatgt aaaagtttag attttaaaga tttaaataat
      661 aataaaataa ataatgttta tcaaaataga ttaaataaca ataaattaaa agtttaatta
      721 ttctaataaa aaaatcttta tttatgatgg aatataggat atatctatgt tgtaatttgt
      781 aaaatcttcc tatatcatat aaaattaagt attgattagt ctattacata aatatagaat
      841 ataaatatat ccatttaata ataaggagaa gagatatgaa agagtatgta gttgtggatt
      901 tagaaactac aggattagat ccatataaag gttgtgaaat aatagaaata ggaataacag
      961 aaataaaaaa tgaacaaatt gtaaaaaact attcaaggtt gataaaacct aaggggataa
     1021 tttcttcatt tataacagaa ttgacacata taagtaatga aatggtagaa aatgaagaac
     1081 cactagaatt agttttacct agatttagaa aatacatagg tgatagaact ataattgcac
     1141 acaatgcgaa atttgattta aaatttttaa attactatct tagaatgtta gatttagaac
     1201 caataaacaa ttatatatgt acagtagagt tattaaaaaa atgtaaaagt tataaaggaa
     1261 aaaacaagaa acttgaaaca gcttgtgctt attacaacat agagaatata aatgctcata
     1321 gagctgacag tgatacatta gcaactgcaa aactattttt gaaaataaaa gatgaatatt
     1381 aattataaat atagagaaaa ttagtgaaaa tatcctttat aaaattgacc cttaagctta
     1441 caattttgtt ctttgattta ttttgtagaa caatgtataa tgtaccttat atgaagaagg
     1501 gagactaaaa tgggttatta caatgataat gataataata aaataagacg taaaaaaatt
     1561 agtaacagca aagatagtaa taaaaaatct aaacattctt atactccggc aaataaaaaa
     1621 aataaaagtt ctaagacaag ttctagtaaa agaatgagtg aaaaagtaga ttcacagaat
     1681 cttataaaaa gagatagaga tagttttaca agagataatg taagaccttt aaatacagat
     1741 tacaatcaaa gagaaagaag aagagtcgag tataaacaaa gagagaaaaa attaaaaagt
     1801 aaaaaaagaa aaaaacaaaa aaggattaga atagttagag cattattatc tattatgtta
     1861 gttgcactaa ttatattttc agtggcaggt gcaatattta cagtttcagc tataaaaggg
     1921 tcaccagaag ttactaaaaa gttaattagg tctaaatata taagtagtga ggttgtaagt
     1981 ataaaacaaa tgccaaatga tttaaaacat gcaatagttt ccatagaaga tgagagattt
     2041 tataagcata aaggagtaga tgttatttct ctagttaggt cagtattaaa caatgtgttt
     2101 acagatacaa cacaaggtgc aagtactata gatatgcaag tttcaaaaaa cctgcttact
     2161 agtaatgata aaactatgaa gcggaaaata agggatatgt ataatgctaa gcagatgaat
     2221 aagattatga ctaaggatga aattcttgaa gcatatttaa ataatatgta tctaggtaaa
     2281 aatgcatatg gagctgcaaa gggagctgaa gtctactttg gtaaaaaagt tagtgaatta
     2341 aatttagctg agtgtgctat gttagctggt atgacaaata atcctgcaag gtatattgac
     2401 catggagagg ctaagaagag acaagtgatt gtactgtata agatgcatca gcttggttat
     2461 ataactgata aggagtatag aatagcaaaa gctgatgaca ctccatttaa atcagaaatt
     2521 gattaaaatt gtaagaatat agtattttca atagtttaaa caaacaaaaa tgtaataaaa
     2581 aaggtgattt gccacccatt tgccaccata attataatct aatggcaaat tgttttaatg
     2641 tacaaactag ataatagaat attgtcttaa atgctatatt atgctaaaat ttaattgtat
     2701 tttgtaaatt aaaaaactta taatttagtt ttctagggtt ccgcatttta taatggtcag
     2761 gtccaagaga aaacgcgcaa ttgttgtgta cacggaagga taaaagcctg ggagatattt
     2821 aagaatattt ctataggctt ttttattttt acatatttta aatttgagga ggttttctaa
     2881 tgaaaaaatt tgttattgag gatgcatttt ggaatctatt tcctagtgca aaaattggag
     2941 ttgttatttg taatgatata gataactcta taaaagatga agactataag aaaataattt
     3001 tgaagggaga agaagaagca ttaaaatact tggaggactc agaatttagc aacaaccaag
     3061 tgattaaagt atggagagaa gcatttaaaa aatttaaaac gaaaaaagga gcaaggtctt
     3121 ctattgaagc attattaaaa agagttcata atggaaataa tataggaact attaaccctt
     3181 tagtggatat ctacaatttt atctcattaa aatatgcatt accttgtgga ggggaggata
     3241 tagataaatt tataggagat attaggctaa ctagagcaat tggtaatgag gagtttatcc
     3301 cactagggac agatgaaaat tcatttccat atgaaggaga aataatttat aaagatgatg
     3361 gtggagcaat ttgtagatgt tggaattggc gtgaagcagt gagaacaatg cttactgaag
     3421 atactaacaa tgccttttta tgcattgaat tagtagatga aagtagattt aaagaatttg
     3481 aaaatgcttt aaatgaatta gctaaaatag ttcaagaaaa attgggtggg aattgtaaaa
     3541 tttcaatttt agatattgat aataaagagg tatcagtgga ttaatatgga ggtgctgttg
     3601 taatggacta ttctagttat aaagatactt atatttaggg taagaaggtc tattttagat
     3661 ataacgatta tcaaaggata taatatagaa aataaaaatg tagaatataa aaagccttta
     3721 aaataagaaa aaaatgtatt ttaaaggctt ttattatact cttttttatt tgcataatta
     3781 acaataaaaa tatattgtta attataatat ttttattgaa aaacgatata atataatgta
     3841 ttatactata tataaaatat tttaaaggag ttggcatgat gtctaataaa cttataaaaa
     3901 atttactaag tggtatatta caaattttat tttttctttt aggattagta attgttgcag
     3961 gaggatttaa aagttttatg tacctctgtt ttagtggaga agcaactcta caaggtacta
     4021 tttatggaat attaatgttt attttaggtg tatcatattt tatcattata aaatcattga
     4081 tagaagtatt aggtagttct gaatatagcc tatttgtaaa agagaatgta aaaagattta
     4141 ggattattgg atatcttttg cttttaaact ctctaattga gtttatttca acttttggta
     4201 ctactggaaa aggaatgaga tttctagatt taggatttgg attttatttt actgttccag
     4261 tatttgtata ttttataact tctcttatga gttttgtaat tgcagatgga tttgtaaagg
     4321 caataaagat aaaagaggat aatgatttaa ctatttagat gtgacacttt aatatatagg
     4381 agataaataa cccgaaaaga gtgacta
//
LOCUS       DAYATN010000211         4350 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.215, whole genome shotgun sequence.
ACCESSION   DAYATN010000211 DAYATN010000000
VERSION     DAYATN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.215"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            407..2506
                     /gene="nrdE"
                     /locus_tag="ACGI8A_003572"
     CDS             407..2506
                     /gene="nrdE"
                     /locus_tag="ACGI8A_003572"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFH0181536.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDKWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2519..3490
                     /gene="nrdF"
                     /locus_tag="ACGI8A_003573"
     CDS             2519..3490
                     /gene="nrdF"
                     /locus_tag="ACGI8A_003573"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFH0181537.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3566..4192
                     /locus_tag="ACGI8A_003574"
     CDS             3566..4192
                     /locus_tag="ACGI8A_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0181538.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1747 a          465 c          791 g         1347 t
ORIGIN      
        1 taaataaaaa acttctaaat aatatataaa atatcatctt gaaagtactt ttttcaatat
       61 atcagaatat atttttaagc tatttagaga taatttaaat ttttaaacag gacataataa
      121 gcttgtattg ttttatattt gagttgataa aaatttcttt aaataaaaat aatgtcaatt
      181 ataaagtaat aaaagtttag atataaaaaa atttgtgtat atactatatg tagtatataa
      241 tagataaagc actgactata tgtagtgctt ttgtttttga aaaatactta ttgcatttct
      301 ataattatag attaattttg ggatttcaac tgataagtac caaaagaaat aataagcaaa
      361 ttaagtgaaa ttatggcaca agattatgtt aaaaggacgg taaataatga attggataga
      421 gcttaacaat caagtgataa ttaaaaatga acaaggcaag taccaattgg aaaaggataa
      481 ggaagcctta agtagttaca tagaagaatt tatacagcca aagttgagaa aatttaataa
      541 cctagaagaa agattgaagt atttaataga tgaaggttat tattcaaaag aagtaataaa
      601 tcagtatagc atagatgtta ttaaaaatat atatgatatg attgataaac ataagtttga
      661 atttcaatca tatatgagtg caaataagtt ttatcaaaat tatgcattaa agagcaatga
      721 tggaaaggaa atattagaaa catataatga taaagtgtta attgtagctt tgacactagg
      781 aaatggcgat aaagatttag ctttaaactt ggcagataaa atagtaaaac aagaatttca
      841 accagctaca cctacttttt taaatgctgg aagaaaaagg gctggagaaa tggtatcatg
      901 ttttttacta tctgtagaag atagtacaga aggtatatcg tatgctatat cttcatcaaa
      961 tcacttatca aagattggtg gaggagttgc actaaattta tctaaactta gagcatctgg
     1021 cgaatctatc aaagatatag aaggtgcagc tggaggtgtt gttggagtag ctaaaatgtt
     1081 agaacaatca tttagttact ttaatcaaat gggagctaga caaggttctg gtgcggtgta
     1141 ccttactgta ttccatcctg attttgaatt gttgatggat actaaaaaga taaatgcaga
     1201 tgaaaaaata aggcttgcta cattatcatt aggagctata ataccagata aatttatgga
     1261 gttggcagaa aaaaatgaag tagcatatgc attttatcct catactgtgt ataagaaata
     1321 tggagtttct cttgatgaga tagaaatgga taagtggtat gataaactgg ttaatgatag
     1381 tgatataaga aaaaaagaaa taaatcctag gcagatgttg acaaaaatag cacagatgca
     1441 acaagaaagt ggatatccat atgtagtgta catagataca gctaatagag aacatacatt
     1501 aaaagatgtt ggaattatta aaatgtctaa cttatgttgt gagatatttc aatatcaaac
     1561 accttcagaa atagaggggt atggaggaaa aaatgaatgg ggacaagata taagttgtaa
     1621 cctaggttct ttaaacatag ccaatgtaat ggataataaa actatagaaa gtacagttga
     1681 aacagcaata agagcactgt catttgtagc agatagcaca gatataaaac cagtgccaac
     1741 ggtaagcaat agtaacagca agtctcattc tataggatta ggagctatga atttacatgg
     1801 atacttagta agagaaaata ttttatatac ttcagatgat gctatagatt tttctaatgt
     1861 attttttgca atggttagat actattcaat taaagcttct atgaaaatag ctatagaaag
     1921 aaatcaaact tttgaaggat ttgataaatc agaatatgtt aagggaagaa atagcaaggt
     1981 tttatcaaaa tactatgaac aatcttatct acctaagtca gaaaaggtaa gagcattatt
     2041 tgaagggatt tatataccaa caaaagaaga ctggacaaaa ttattggatg aggtaaaaga
     2101 aaaagggata tataatgcat atttaatggc agtagcacca actcaaagca ttagttatgt
     2161 tcaaaatgct acatcaagta taatgccaat tactgagcca gtagaagtaa gaacatatgg
     2221 agactctact acaatatatc caatgccatt tttaacaaat gataatatgt tgtattatca
     2281 atcagcatac aggatggata tgagaaaggt catagattta gtggcaacag ttcaaaacca
     2341 tgttgaccaa ggtatatcaa caacactgtt tgttacagat gaaaagacaa ctagagatat
     2401 agctagacat tacatatatg cctataaaaa aggtttgaaa agtttatatt acacaagaac
     2461 aaagatgaca agagatactc atgagtgtct agtgtgttca gtatagggag agaaatatat
     2521 gacatttaat ttacataaaa tacataatgc agttaactgg aatagagaag aagatggatt
     2581 tacacaagct ttttgggaac aaaatgtaaa acaattttgg cttcctgaag aaatatctgt
     2641 ttctaaagac ataaaggtat ggaatgaact aagtaataaa gaaaaggaat tatataaaaa
     2701 agtattgggt ggtttaacat tactggatac aaaacagggg aataatggaa taccatctat
     2761 gatgagttta acagaaaacc tacaaagaaa agctgtcttg tcttttatgg ggactatgga
     2821 agaaatacat gcaaaaagtt attcttctat atttatgact ctactttcta atttagaaat
     2881 agatgaatta tttgaatgga tagaaacaga accaacatta caaagaaagg ctgatttagt
     2941 tttagctcaa tacgaaaata ctacaaatca agagggatta tacctatcaa tggttacaag
     3001 tgtattttta gagagtttct tgttttattc aggattcttt tatccattgt atctatctgg
     3061 tcaaggaaag atggtagcta gtggagaaat aatatcgctt attcttagag atgagtcttt
     3121 acatggtaag tatataggat tgctagcaca agaaatatac gattcatttg ataaaatgga
     3181 taaggagatg ttagaagaga agatgtattc tatactttat agccttatgg agaatgagat
     3241 agagtataca aatgttatat acagagaaag tgggcttgaa aaagaggttg tgaacttttt
     3301 aaaatacaat gcgaatagag ctttagaaaa tctagggttt gagaagctat atactgtaga
     3361 tgctataaat ccaattgtat taaatggtct tagcactgaa accaaaacac atgatttttt
     3421 ttcaactaag ggtaatggat accaaaaagg agtgtatgag gaattagagg atgaagattt
     3481 tataatataa tttataatta tgacacactg ttgtcacttt tagcgtgata atatcaatgt
     3541 ataaaatata tatggaggta tttttatgaa atatgaatgg agaaaaagag ataaagagtt
     3601 atatttacct aaaaagaatc ctgtaatagt tgaaatacca gaacagaaat ttatcatgtt
     3661 aaaagggagt ggagacccaa attctaaaga atttactgaa gcaataggag tactttattc
     3721 tttggcatat gcaataaaaa ccatgcctaa aaaaggaatt gtaccagaag ggtattttga
     3781 ctatacggtt tttcctttag aaggtatatg ggataaggga gaaaaaagta aaggcttaga
     3841 tgtacttatt aaagaagatt taatttacac tataatgata cgtcagccag attttgttac
     3901 acaagacctg ttggataaag ctactgatat agtaaagaag aaaaaacctc ataatcttct
     3961 tgaaaaagtt agatttgggt ctattaatga agggctatgt gttcaaatgc ttcatatagg
     4021 ttcatatgat aatgaaccag aaagctttga cattatgaac gaattttgca aaaaaaataa
     4081 acttataaga aaatcttaca cacatcgtga aatttatata agtgatgcta gaaaagtatc
     4141 gcctgaatca ttgaagacgg ttttacgttt tgaagtagaa agggaagtat aaatattttg
     4201 aataatttat aagagttata aaaagtgaag tctgttgaga cttcattttt tgatgcaatt
     4261 ttaaaattaa atttgatgtt tcataatatt ttaaaaactt tctactttta ttagtgtgaa
     4321 taactgttgc tattaaataa taatactaat
//
LOCUS       DAYATN010000212         4239 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.216, whole genome shotgun sequence.
ACCESSION   DAYATN010000212 DAYATN010000000
VERSION     DAYATN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.216"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            253..591
                     /locus_tag="ACGI8A_003575"
     CDS             253..591
                     /locus_tag="ACGI8A_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HFH0181539.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(998..2434)
                     /gene="miaB"
                     /locus_tag="ACGI8A_003576"
     CDS             complement(998..2434)
                     /gene="miaB"
                     /locus_tag="ACGI8A_003576"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFH0181540.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(2682..4118)
                     /locus_tag="ACGI8A_003577"
     CDS             complement(2682..4118)
                     /locus_tag="ACGI8A_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HFH0181541.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT         1380 a          667 c          450 g         1742 t
ORIGIN      
        1 agtttctaaa tttaacaatt ctaaaatatc aataactata ttttgtagca cttttttatt
       61 ttattattgt ttgaatattt gatggttttc aatgcaattt caacataaat actatatatt
      121 agtgtatact gttgatgaat taaagtatga taatctattt aataaagtat gaagtaaatg
      181 attttaatgt gcctttatgg taaaattact aataagtaat aaaattaatt aaaatatatg
      241 gaggtcctta taatgtttgc tgtaggattt gttgttataa ttgcacttgc ctttttcatt
      301 ttaagtgtac tattatttaa tggaaaagcc agtatgctac tagcaggtta taactctatg
      361 agtaagaaag aaaaagcacg atacgataaa aaaaaattat gtagaagttc tagcattgtt
      421 acttttattg tctctataat gctatttatt atggcgtttt tgggatatag aatagagagt
      481 ggtcaaatgg acgaaaaaaa aatggttccc ttttctataa tatttataat agtcatactt
      541 tccagtgtat tttttaatat cctctactct aacaaaaaat gtaaaaaata aatagttgta
      601 agttgagcag ttaattataa aagattgact gctttttcat gtatattgac tactcttatt
      661 aaatatttta acatttaaaa atctatgtaa aaataatgaa tatagtacaa acttctaata
      721 atgattttac ctttgtggtt acagtccact atatataatt taatatgata acagggaatt
      781 attaatgaca actaagtaaa cttttataaa atataataaa acaaaaatag atgtacaatg
      841 aaattatatc ttatatcaaa tttattttta ttaatagcaa aaacttacat tagcataaca
      901 tatgacaaat tcgcttaaaa taaaaatttt taatataatt tgttctatag tacatctatt
      961 ttgattagtt tacataattt tattattttg tacaatatta attatttact aatatcccat
     1021 ttaatgaaaa agttctaggt tcagtaattt tgaccataac taatttacca attaaatcat
     1081 catttccacc ttcgaaattt acgagtttat tttgtctagt tctaccagca aacttatttt
     1141 cattattttt acttcttcct tcaactagaa cttcaactat tctatcttta tatccatcat
     1201 ttatttctgc agaaatccta tttacagctt ctaacaccct attaaatcta tcatgcttta
     1261 tatcttctgg tatttgattt tccattttag cagcaggagt tccctgtctt ttagaataaa
     1321 taaatgtaaa agctgaatca tatctaactt tttcaaccac atctaaagtg tccaataaat
     1381 cttcttctgt ttctcctggg aaaccaatca ttaagtctgt agaaaaagca atacctggaa
     1441 cttccttttt agctttttct attatttcta aataatattc ctttgtataa tgtctattca
     1501 tttttttcaa aaggctactg cttccgcatt gtattggtag atgtaaaaac tcacacacct
     1561 tatcacaatc tctaattgcg tatattactt catcagatat atcttttgga tgagatgtca
     1621 taaatcgaac cctttcaatt ccttctatat cattcacttt tcttaaaagt tctgagaatg
     1681 aaactggatt ttcaagtgtc ttaccataag aatctacatt ttgtccaagt aaagtgattt
     1741 ctttcgttcc atttgcaaca agctctttta tctcgttaat tatatcttca ggagttctac
     1801 ttctttctct tcctcttgtg tatgggacta tacaatatgt gcagaaatta ttgcatccat
     1861 acattatatt tacaaatgct tttaattcaa attttctatt acttctaaga ccttctatta
     1921 cttctccatc cacatcccat acatccacta acattttgtc agaatttatt gattctgtaa
     1981 gtaattgcgg aaatttatat agattatgtg ttccaaatat aaggtctaca tgtttatatt
     2041 tttttcttaa ttcctctaca acatgtggtt gttgcatcat acatccacat acagctattt
     2101 tcatatctgg attttttcct tttagatgtt ttaattgtcc taagttacca tatactttta
     2161 gctctgcatt ttctctaact gcacaggtgt tatatattat taaatcactt tcttcaacca
     2221 tcattgacat ttggtatccc atctcttcta acattgaaca taatttttct gaatcatgtt
     2281 cattcatttg acacccaaag gtttgagtaa acacgagttt tctctttcca gttctgttga
     2341 agtattcttc attttgtctg tgtatttgtt ctatatattt atcttgttct tgtattttct
     2401 ctataggtat tgttacttgt tttcttttgc tcacgtctat tcctcctagt taatttctta
     2461 tattacacat aaccttaatt atatcaaata agaaaaatct tttaaagata ttattatttt
     2521 tctagttgtg ttttaatagt aaatctaatg atttatatat actatatttc taaaatataa
     2581 aatcatataa ttattataat aataaaaaga tgttttaaat taaaacttaa aacacctttc
     2641 cttaaataaa atatatgatt tactattaaa tacaattaca tctattcatt ttcaattaaa
     2701 taacaatcat ttaatattaa atattgacct tcctcaaccg ttatatatct tcttcctgaa
     2761 aaagtatcat tagtaactat tccctctgca ttacctaagc tatcatttct aacttcataa
     2821 tatgcatcag aagaatttga cattataata tattctccag caggaatttc taatccaatt
     2881 ttatacatac catttccaaa cttaccattc ttggccttct gggctttagc atactttgct
     2941 aatgtcattg ttgaatcctc aattttaata tattgaccat tctttaaggt gatatatcta
     3001 gtcccactaa aaatatcatt tgaaagtata ctatcagcat tacctgtact atcacttgta
     3061 acttcataat aagaagcata acttcctgag tttgaagtta ttaagtattc acctgctgag
     3121 atatcttttc caaccttata cattccatct tttatagtct ttgtagtagg tttaacattt
     3181 tttacaaccc cagtattaat atctccaatt ccatttgtta tatttaaaca ctgttgtact
     3241 atatttgaat ctatatatcc tatagaagtt atcttcttag cattttttaa aatgctttta
     3301 ttgctaccat cattaactaa aaccattgga ccatgttttg ataaagaaga tcctacaagt
     3361 gcatttgtta attcataagc tccagctata taaaactctc tagcatcctt ataaaactta
     3421 tttattattg ctttattagt ttcaaaacga gtactccccc ctagtctagt agatttagta
     3481 ttgtttacta aagtggtact cattgaagct gttcctccta ttacataaga ctttaaacca
     3541 ctagttgaaa aaggtatact ttgtccatta gtaagaatta ttggtgcttt atctcttgct
     3601 gctacagatg caatacttat agcatctgct tctcctttat atgcattggt tagcataaca
     3661 gtattgactt tttttattga atttatttct tttgcaacat tataacttgt ttttattctg
     3721 tcatttccct taattcttac aactttcatt ttcttagatt ttaatgaatt ttcgacagat
     3781 gtactaatag aatatgttcc tccaattata taaacttttg atacattttt taatcttgca
     3841 gatgtctcag ttggtatagt atttttttca gttaatagta taggggcatt taaagctcct
     3901 gctagtccac ttgcacttaa tccatctgca atagagttac tagtattaat taatatagca
     3961 gttttatatg catttttatc tgctatttta cctgcgattt catacttatt ttttccttgt
     4021 attttttcta cagatgatat tgcatctact aattgaatat ttgcaaatac aaaagaaata
     4081 cttaaagtca atgttaatat tcttttatag aatttcataa taaaattgtc cccctcataa
     4141 taaattttta atatataaat acatattctt aaattagtta tgtacttaac aaacttatgt
     4201 aaaattgcaa attatattaa gcatattaca aataaatat
//
LOCUS       DAYATN010000213         4216 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.217, whole genome shotgun sequence.
ACCESSION   DAYATN010000213 DAYATN010000000
VERSION     DAYATN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.217"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..1312)
                     /locus_tag="ACGI8A_003578"
     CDS             complement(<1..1312)
                     /locus_tag="ACGI8A_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0181542.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAI"
     gene            complement(1562..1999)
                     /locus_tag="ACGI8A_003579"
     CDS             complement(1562..1999)
                     /locus_tag="ACGI8A_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HFH0181543.1"
                     /translation="MGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVYVFSVIGSTL
                     VSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGIILFVGIPLPT
                     TGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(2031..2660)
                     /locus_tag="ACGI8A_003580"
     CDS             complement(2031..2660)
                     /locus_tag="ACGI8A_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HFH0181544.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(2694..3581)
                     /locus_tag="ACGI8A_003581"
     CDS             complement(2694..3581)
                     /locus_tag="ACGI8A_003581"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HFH0181545.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(3610..>4216)
                     /locus_tag="ACGI8A_003582"
     CDS             complement(3610..>4216)
                     /locus_tag="ACGI8A_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6080 domain-containing protein"
                     /protein_id="HFH0181546.1"
                     /translation="FEFKLKIGKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYD
                     TKFISGEPILEKIAMIIERLLYSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFC
                     VLSVIENSEKRVPKLCMGIIIFNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKA
                     LRKNEKNMYNIFLSLLLVTIVISNVNGFIEILNIGIKSYPV"
BASE COUNT         1524 a          713 c          449 g         1530 t
ORIGIN      
        1 aaattgctgt tatagtagcg gctgttatat ctgcaactgg ctctgctaaa attactgcaa
       61 aaagtttttc attaaagaat gttggaagta taaatattaa aggtattaaa agtacaatct
      121 ttctaagtag agctaaaacc aaagatattg ttgcttttcc aagtgctaaa aatgtctgtt
      181 gacaagctat ttgaatccca aacataaaag caccagcaaa atatattttt atactccaac
      241 tagtcatact aagtaactgc ggattcttat taaatatact aacaaaaaat gcaggaaata
      301 tcattaataa aagccataaa atagtagaat atgtaaaaca tgacaataaa cacaacttaa
      361 atgtcttttt tactctatca agttgacctg aaccataatt ataacttatt attggttgtg
      421 ctccttgtgt aacccccata ttaggaagca taagaatttg cattatactg ctcattatgg
      481 ccattgaacc tactgctaaa tctcctccat acatttgtaa tttactattt aaggctataa
      541 gtactaaact ttctgtagat tgcattataa atggagagat tcccaagcca agcactggta
      601 agatgatact tgcttttggt atcatatact tcttttttat tttaagtata cttttctttc
      661 caaagagaaa cataagtacc caaatagcag aaacgccttg tgcaattata gtagctaatg
      721 cagctccttt tacacccata ttaaagccaa atataaaaat cgggtcaagt attatgttta
      781 ttaaagcacc tatagcaact gtcatcattc cagttgtagc aaacccttgg gtatttataa
      841 aagtattcat acccattgaa atctgaacaa acactgtacc tattaaataa atatttaaat
      901 agtccattga atatccaatt gtagattcac ttgctccaaa catccaaagt aaaggctctt
      961 ttccaatctt aaaacctata gtcaaaagta ttgctaaaat caccaataca gaaaaactgt
     1021 tactcattat tttctctgcc tcatcattgt tttgctcacc catttttata gcagctaatg
     1081 gacctcctcc cataccgatt aaataactac atgcagttat tataattatt attggaaatg
     1141 ccactccaac accagccata gcaacttctc cattttccat tctaccaata aaaatcctat
     1201 cgactatgtt gtataaaaca tttacaattt gtgcgataat agcaggtgta gcaagtttaa
     1261 ataataattt acccacactt tcactaccta agttagtgtt tgaattttcc attttaaaac
     1321 ctcctcaatc caataattaa aatgtatcac taaaatttta ttctatataa aaatttcatt
     1381 ttattagcag acaaatttag agtactttcc ctacaagtaa tactatacta tatttctttt
     1441 gccatagtaa acattttaga actaactgaa gttaaatact ttatctcatt ttttatttat
     1501 tattaaaaat ttttcacata tactcataaa aaaactatcc aagtatatta cttagatagt
     1561 tttatattat atgtaaagaa agagctgaaa ctataacccc tgataacaaa ttgccaagaa
     1621 atactcccat aaaagcatct tttattctca tttttaatat ggaagctata gcagatgcag
     1681 tccatgttcc tgtagttggc agtggtatac ctacaaatag tattatacca attatcgaaa
     1741 cactttttaa tttcttcatt cttgagttta tttttctatc aattacatca gcaatccctt
     1801 taaatagttt cttccctctt aaaaattgta atacatgtct gaatgtcaat atcaagggaa
     1861 tcgataccaa tgtagaacct attacactaa atacataaac ccatatagga tttaagccca
     1921 ttgcaatacc tataggaatg gctcccctca attctgttac aggaaccata gataaaatta
     1981 atatgtgtaa atatcccaaa ctattccctc ctattctaag tgacattaag ttatgaattt
     2041 atttgctcta taacctcacc atccttttta ctaactaaaa atgcattttt agtcaatgaa
     2101 agcattggta aatcactctt agagtctgaa tatgcatatg aattttcata atcaatctct
     2161 atcccaagtt tacctaatac agcctttatt ctttttactt tttcaattcc cttacagttt
     2221 tctccaatga attgattttt atatcttgaa ttttcataaa aaaactccgt gcctataacc
     2281 tcgtctgcaa gaccataata cttaaaatat ttcatatatg catatggaga tgcagttatc
     2341 ataattataa cattatcttc ttctttattt ttgtaaataa cctttttcat agattcgtta
     2401 atttttttca ttatgcaatc atcaaaaaac ttttttaaat cttcttcact aaaatctact
     2461 attggataaa agattgcctc ttttgccttc ttcaagggta tcattctaat taaatacata
     2521 aatagaataa aaataatgta tggcaacttt ataatagttt taaaggtttt attttttaaa
     2581 gagtaaataa aaaaatctat catgctatca gtatttataa cagtataatc tacatcaagt
     2641 aataatacac tttttttcat aagtatttcc ccatcctatc actaaaaata tcactaaaaa
     2701 atcaatatta ttaaacatgt aattatccat aagattacgt ttatttttaa agcattatct
     2761 tttaggacaa tatcagtagg attattttca tttgtagtgt taattatata attatatcta
     2821 agtacaccat aaactacaaa tattgttgta aatatcatat gtggcttttc tggattactt
     2881 gtagaataca atgcataaca tacaattgta gaacttaata taactatcat catttgattt
     2941 agattttcta tgtcataatc ttccaatatt tttctatgtt caactgcatc atctcttaga
     3001 gtttctattt cttttttacg ttttccaaga gctaagtaca gagataagaa aaatgtacat
     3061 aaaattatcc aatttgataa actaacactt atggctacac atcctgcata aaccctaagt
     3121 ataaaaccaa gagatattga aaacacatct aatagtatta tattctttag tttaagagaa
     3181 tataatatat tgtttatcaa atagagactg agaattatag aaagattaaa actaacaact
     3241 gttgataaac agactgaaat taacgctaat actacaaata gtatattagc actttttttg
     3301 cttattcttc cacttgccac tggtctatta cattttttag gatgtttttt atctttatcc
     3361 atgtctatag catcattcag tgcatatact gatgatgaca ttaggcaaaa acacacaaac
     3421 gcaataaacg tatttaaaaa taaatttata tttagtatgt tgtttgaaaa aattatagct
     3481 gaaaatacaa agaaattctt tatatattgc tttattctca ttaactttat ataatctgaa
     3541 attttagtaa gaagccttat acttgtattg gtctgtttca taaacttact accctttcaa
     3601 tattattttt taaacaggat atgattttat ccctatgttt aaaatttcta taaatccatt
     3661 gacattactg attactatag taaccaataa cagcgataga aatatattat acatattctt
     3721 ttcatttttt ctaagggctt tcgtaaagta agccaataaa attataactg caaaactaaa
     3781 atttatagcc ataatacttg aattgactag attatatcct actataccat gtaaagttag
     3841 attgaatata attattccca tacatagctt aggaactctt ttttctgaat tttcaataac
     3901 tgataaaaca caaaaacata aagctaataa tgtaagaatt actggtattg tagcaacttc
     3961 tccattttgc aaaaataacc cttttttaat acttgctcca ggaaaataaa aaaccgaata
     4021 caataatctc tctataatca ttgcaatctt ttccaaaatt ggttctccac ttataaattt
     4081 cgtatcatac tgtattacat ttccaaggtt cataaaactc tcataagact tcgttgaaac
     4141 tacacaaata tatgccatta ggataaatgt aattaaatgt tttaacattt ttcctatttt
     4201 taatttaaac tcaaat
//
LOCUS       DAYATN010000214         4216 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.218, whole genome shotgun sequence.
ACCESSION   DAYATN010000214 DAYATN010000000
VERSION     DAYATN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.218"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..786)
                     /locus_tag="ACGI8A_003583"
     CDS             complement(<1..786)
                     /locus_tag="ACGI8A_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0181547.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQ"
     gene            1127..1504
                     /locus_tag="ACGI8A_003584"
     CDS             1127..1504
                     /locus_tag="ACGI8A_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0181548.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            complement(1941..2204)
                     /locus_tag="ACGI8A_003585"
     CDS             complement(1941..2204)
                     /locus_tag="ACGI8A_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181549.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            2520..3095
                     /locus_tag="ACGI8A_003586"
     CDS             2520..3095
                     /locus_tag="ACGI8A_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181550.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            3193..4191
                     /locus_tag="ACGI8A_003587"
     CDS             3193..4191
                     /locus_tag="ACGI8A_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HFH0181551.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
BASE COUNT         1611 a          408 c          598 g         1599 t
ORIGIN      
        1 ctgttttatt ttatattctt ttaataaaga cattaattct ttgggagaag atatttttct
       61 aacagagctt tctgtctttg gctctttgag aataacagaa ccattaattc gggaagttat
      121 cttatttact gttattgtat tttcttcaaa atcaatatta tcccaagtta gacctaaaac
      181 ttctgaaatt ctaagaccta atccaatagc caaattaata ggtaattcca tttctgtatt
      241 ttttgctaaa tctaataatt ttatcatttg ttctctatca taaatctcat ttttaaattt
      301 tttatatctt ggagcctcta tactatccat aacattttct tttattaatt tgagtctata
      361 agctcttttt attgcaagtt tcaatatatt tatatgaatc ttaattgatt gaggatttag
      421 tttataagat agtttatcta tgtagttttg tatgtgaacc ggatgtaagt cttgtatttt
      481 atattttcca atagatggat taatgtaatt cttacaaata ctaacataat tattatatgt
      541 agctaaagat atattttcac tatatttatt tagaaaatct aatagaaatt ctgacactgt
      601 aatttcgctt ggtactaaaa aaccatcatt atataagctt tcttttacct catttagtct
      661 tttacttgct tccctttttt tatcaaaagt acccatattt ttttgttttc ttttacctgt
      721 cttttcatct tcaaattcca ggtaaacaac atagttttta tttctttttc taataaatgc
      781 attcatatat aacccaccaa tcaaaatttt atacatttat tataacatat atatatcaca
      841 aaataatttt gaaatagagt tgcatacatt ttaatacatt gtatatattt atttaaaaaa
      901 tgtatatatt atgtcaaata ttagaatttt aatttgataa tctgttatac ttatagaata
      961 gatatttatc tttatattaa tagattgtgt gtttaatttt gaataaaaat ataaattttt
     1021 tgtattaata aatttggatg agatatttta aataaaacct ttgactcgaa aagtctatta
     1081 atatagtatt taattatgta gaagtttgag aggagtgtaa aatcatatga ctattactaa
     1141 gataccacca gcagaattaa aagtcatgaa atttatttgg agttcagatt ttacagtagc
     1201 atcaaaagat gtcattgaag ctatggaaaa actatataat tggaagcaaa ctacaacact
     1261 aacactttta tcaagattag taaataagca attcttaagt gcaagaaaaa ttgatagaca
     1321 tacacattat acagttctta ttggagaaga agagtattta cattttgaaa caaaaacttt
     1381 tttagataat atgcatggaa gttctattga gagttttatg aaatccttgt tcaaaaaggg
     1441 ggtaaatgaa gaagaattga actctgttga aaaatgggtt aaggatatta aaaaagatga
     1501 ataatttggt gtagttaatt atcataaaat aaatagtttt tacaataatt agttaaatga
     1561 atcaaatata aagttttatg taaaatttgt gatttttata aatatttttg tcttgttatt
     1621 taatattaat acaataagtt tgatgtaaaa tcaaacctgt ttgaatttaa tttatattgg
     1681 gtttgtcatt aatattattt tagtttgata ttgtatacga atttaaaagt cagaattttt
     1741 gtatattaat catgtaaatt aattgtttta gatataaagg gcataatttt atttttgttt
     1801 tgattatttc tagggtaaga taagtataca atagattgta ttaaacatgc ttatattgat
     1861 agatttgaga taaatattga gatatattaa taattattaa tataaaaaag ttcctagttt
     1921 ttaggaactt ttttatttga ttaatacaga gtatgagttt ttgcttcagc tatagctctt
     1981 gctgcacttt taggtttttc agtattttct tttttgtaaa atttaatcac ttcttctaaa
     2041 tctttatttt caatattatg ttttttgttt tcaagaatat aatttacaat tgattcattt
     2101 atcaaatctg tcaaaatatt attttcgtca ttagttatct tagacatttc ttgtaataaa
     2161 atttcattaa cttctactgt aatttgtttt gtatttttta tcatatcaac cacctcttaa
     2221 gaaaattata tcatctcaaa aaaataaatt caatatgttt taaaattata gaattttaat
     2281 tcttaaattc taaaaaagag aaaattagat aaagtagttt tagattttaa aagattaata
     2341 aaaaatattt aaagataaaa gctttattct atatgattct aagatagagg atttataagt
     2401 gtttaaggtg aattaaatat aagacaactt cttaaagcaa atgggtgtgg tatttaaatc
     2461 taaatgtaga catataatat taaatatgct aatatatcaa aataataagg ggggaattta
     2521 tgaagcttaa agaagcattg attacatgtg ttgtatgtag tgtatgttta actccattat
     2581 tggtaaatgg agaaacaaaa atagatgcag atcattgtaa agaatctata gtaaatatag
     2641 atactatagt aaagtcaggt aaaagcttta attccaagta taatcaaggt atggaagatt
     2701 ataaaaatag tgatgagtat aagttgaaag tacaaaagga gaaactacaa aatcagttag
     2761 gtattaaaat aaagaagtta gtacctataa cttttgaagt tagttattac acaagtctta
     2821 atcaagaaaa tggatatgga gcgataactg ctacaggaga tgatttagaa gatggttttg
     2881 tagcaaacaa tcacttaagt tttggaacta agattattgt agatggaaaa ttaaaaactg
     2941 ttatggatag ggggtctagt aagtatttta gtaattctaa tgcaatagat gtatttgtgc
     3001 ccaaatggag taatgaaagt gaatcagagt actatgatag agtcaataat atgggaagac
     3061 attacaaaaa aggctacatt attgttgaag aatgatgttc tttgaagtaa tatgtattga
     3121 ttgtagtatt taatatatat ataataaata taaaactatt agtacaatga gatatattac
     3181 taggagggca atatggaaca atcgtttata aatggaaaag taaacttatt aatagaaaat
     3241 tacaaagcat taaatgaagt taaaggttct tggcaaatgg gattgataca acatagttgt
     3301 gcacttgcat ttacccttaa gaacaagaga ataagtccta gattagtgga agaacgaata
     3361 gagcttatta aaaaaaatac tggattattc tctaatttta gaggatataa tatgttttat
     3421 atggcaacac tactttcgtt tgaatcaaat ccagaaagta gttttaagat gattttagat
     3481 atatacaaag aacttaaaag tgaaaagttt tggggcgata catatttacc acttacagca
     3541 tcaatagttt atgaaaatag agagaaaatg gattatctta catgtatatc aaaaatgaaa
     3601 ataatatatg attacatgag gaaaaaacat ccatttctaa cttctagtga tgactattgt
     3661 aatatagcac ttattgcaat tcattcaaaa aatttagatg aagatttaga atacatagaa
     3721 aaatgttatg aatttctaaa tgaaaatggt ttttataaag gaaatgattt acaagcatta
     3781 tcccaaattc ttttatttag tgatgataga actatgctta aatgtaaaaa gactattgaa
     3841 cttaaaaaag catttaaaga aaatgattgt aaaatgaatt attatggata tccaataata
     3901 ggagcaattt cattattaga ttatagagaa gatgaaataa tagaagagat aaaaaatgtt
     3961 tcaaataaac ttaaagaaga aaaaggcttt ggaaattggt ctttaggaaa aagtaataga
     4021 gtaatgatta gttctgctat tgtagcttca atttatgcag atttgatacg aaatgagaat
     4081 aatataggct ctataactaa taatatattt ttaaatattg ttgttgctat tcaaattgca
     4141 tgtgtaatgg cagcaacaag tgcagctata gcatcttctt catcaaatta ataagattta
     4201 ttgaagttgg atatat
//
LOCUS       DAYATN010000215         4064 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.219, whole genome shotgun sequence.
ACCESSION   DAYATN010000215 DAYATN010000000
VERSION     DAYATN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.219"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            159..1562
                     /locus_tag="ACGI8A_003588"
     CDS             159..1562
                     /locus_tag="ACGI8A_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0181552.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1653..1893
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1936..2634
                     /locus_tag="ACGI8A_003589"
     CDS             1936..2634
                     /locus_tag="ACGI8A_003589"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFH0181553.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2657..3658
                     /gene="trpS"
                     /locus_tag="ACGI8A_003590"
     CDS             2657..3658
                     /gene="trpS"
                     /locus_tag="ACGI8A_003590"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFH0181554.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1521 a          455 c          732 g         1356 t
ORIGIN      
        1 taataagatt tgatttcaaa gtatttaagg tttattatta aaactgtttt tgaatatgca
       61 aatattttga aaaatatcag tttaataata atgaatctat tttattaatt ttgaaattaa
      121 atttaaaata attaatagag aggggaaatt aaattaggat ggaaaagaaa aaaataggcc
      181 tatggagttt ggtatttatg aatgtatcag ctttatatgg tataagatgg atagctaaat
      241 ctacagcatc aagctttggt ttaggattag gtgcaatacc tatgtggtta ttattctcat
      301 ttatatactt tgtaccagga gctttaattt gtgctgaatt agcttctaca tatcaaaaag
      361 gtgatggagg tctttacgat tgggttaagg aagcctttgg agaaaagtat ggatttttag
      421 tttcttggtt aaactggaca gctaaaatat tttggtattc ttcattttta actttcttag
      481 cagtaaatgt ttcttataca ataggaaatc cagcccttgc tgataataag atgtttgtac
      541 ttatattatc tttaataata ttctgggcat tatctcttat agcaactaaa ggaataagtt
      601 ttacaaaaat atttactaat acaggtgcat taggttcaac aataccaagt gctatactta
      661 taatattttc atttgtagca gtatttgtgt tgaaaaaaca tgatatagct tctacataca
      721 caatagcttc aatgactcca aaacttaata tggatgcatt tgttgctata tcagctataa
      781 tgtttggttt agcaggagca gagactgctg ctaacttcat aacagaaata gataagcctg
      841 aaaagaactt ccctaaggca atattaatat cagctggaat agtagcttca ttatatgtat
      901 taggttcaat tgcaataaca atgataatac caccagacca aattactgct tctaaaggtg
      961 tattagatgc actttctgct gtttgtgcgt ctttaggaat tggttcttgg ttggttcaat
     1021 taatagcttt tggtatagct ttttcagtat taggagcaat agttctttac atagcatcac
     1081 caataaaaat gttatttggt agtgttaaaa aaggaatatt tccagatagt ttagtagagg
     1141 taaatgaaca caagatacct tctaaagcag ttatacttca agcaataata gttactataa
     1201 tactattggt gactacatta atgccttctg ttgatgctat atacaatgtt cttgtaacta
     1261 tgacagcatt aactgcatta ttcccatatg tattgttata tgcttcttat attaaactaa
     1321 gaaaagagag accagatgaa attagacctt atactatggc aaaatcaaca agtacttgcc
     1381 taggtttagc aaaaatggtt ttagttgtaa ctgtagtggg tatattatta tctgcagcac
     1441 cagttatgcc tacatttgct gaaaatgtta tatatgagat agagatgatt ggtggaggtc
     1501 ttctagttat attatctggt ctatggttat ggaatagata tgaaaagaaa acagctaaat
     1561 aaaatgtatt ttaaaaattt cttttggata ttataatata tagttgacaa tttatagtgt
     1621 aaggaatatt ataaaataaa gaaattaata aattaaatcc tgagataaga aatagtaaat
     1681 aaatatattt ttgcacagag aattaaggat gctgagaact taataaaaag tatttattga
     1741 atgggtcttt gaggagtaaa atgaaatgta atttaatatt acagagtatt tgatatcgtg
     1801 tatgcacgtt atagcatata ttaagatata tatattcaat tttgagtgta tataaattta
     1861 ggtggtacca cggaaaatat atccgtccta ttgatttagg acggattttt taatatagaa
     1921 aatttaggag gaaaaatgaa cgtaatagga attataggtg caatggatga agaagttagt
     1981 attttagttg atttaatgga tattagagag actataaaaa aagctagctt agaattttat
     2041 aagggaattt tggaaggtaa gaatgtagtg ttagtaaagt gtggcatagg taaggttaac
     2101 tctgctcttt gtgcccagat actaataagc gaatttaagg ttgatgctat tgttaataca
     2161 ggtgttgcag gagctttaaa cgaaaaactt gatgttaatg atatagtaat atcaactgat
     2221 gctatacagt atgatgtaga cactacagca tttggagacc ctaagggagt tataccaaga
     2281 atgaagactt ctgtatttaa agcagatgaa agacttatag atgctgctta caaatcttct
     2341 gtggaagaag taaagactca taaagtatta aaaggaagag tagttacagg agataaattt
     2401 ataaattcta aagaattaaa agaagaatta gtaaatgatt ttggtggata ttgtggagaa
     2461 atggaaggtg gagctatagc tcatgtatgc tatttaaata atactccatt tgtaataata
     2521 agagcaatgt ctgataaggc agatggaagt gctgatgtta cttatgatgt atttgtacat
     2581 gatgctgcca ataactctaa agacatagtt ttaaatatgt taaaatcaat ataaatatat
     2641 aaggaggtac aaaaaaatga gtgaaaaaaa agttatattt agtggtgcac agccatcagg
     2701 caaaatgact ttaggaaatt atttaggagc tataaaaaac tggactgaac ttcaagacaa
     2761 ttatgattgt tattatagta tagttgattt gcatgcaata acagtacctc aagaccctaa
     2821 agttttaaga gccaatacta tagaattact tgctcaatat atagcttgtg gattagaccc
     2881 agaaaaaaat actatattta tacaatcaca tgtcaaagag catgttgaac ttatgtgggt
     2941 attgaatact atgacttata tgggtgaatt aagtagaatg actcaattca aagataaatc
     3001 acaaaagagt gaagcaaatc taaatgcagg tttattcaca tatccagttc ttatggctgc
     3061 tgacatattg ttgtatcaaa ctgacctagt tccagttgga gatgaccaaa aacaacattt
     3121 agaattagct cgtgatttag caaatagatt taataataga ttttcaccta cttttgtagt
     3181 tccagaagga tactacccaa aaggtggagc aagagtgatg agtttgcaag agccaactaa
     3241 aaaaatgagt aaatccgatt caaatgaaaa tgcatttata ctattagcag atgattcaga
     3301 ttctataaag agaaagataa aaagaagtgt tactgattct ttaggtgttg taaaatacaa
     3361 tgatgaacaa ccaggtatta aaaatctaat tgatatatat tctaatttaa gtaaaaagag
     3421 tgtagaagag attgtaaaca tgtatgaagg aaagggttat ggcatattta aagaagatgt
     3481 ggctgaagta gtatcagaag ctttaagacc tataagagaa aaatatgttg accttcttaa
     3541 taataaagat tatttagaaa aaatatatag tatgggagca gaaaaagccg aaaaacaagc
     3601 tagaaaaact cttagaaaag tttataaaaa agttggattt atagagagaa gatactaatt
     3661 attagctatc tttacttgat aaataaattt taaagttatt aaaaaggtat atgatatgaa
     3721 agagagagtc tcaaagtgaa cttttgttca tgaggcactc ttttttgtat gaaataaaat
     3781 atattttaat catttcaaca atgaaaaaga ggattatctc tattttgaga cagcatcttt
     3841 tgataataaa aaaagatgag cttttgctca tctcaaaggg gttagattat tcttactttg
     3901 gggaaagtag aataaaggta aaagttaact gatgtcaact ttgtcttttt tgcatatcac
     3961 tactgagcat aaaaaaatca gaaaaccata gccttctgaa acaacttcta acatacattg
     4021 tattttatac ccaatatgta atttatgagt taattttaac tctt
//
LOCUS       DAYATN010000216         4024 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.220, whole genome shotgun sequence.
ACCESSION   DAYATN010000216 DAYATN010000000
VERSION     DAYATN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.220"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            168..1121
                     /locus_tag="ACGI8A_003591"
     CDS             168..1121
                     /locus_tag="ACGI8A_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181555.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIVLTVGVIGSILFLYLILRRNVNEA"
     gene            1111..2067
                     /locus_tag="ACGI8A_003592"
     CDS             1111..2067
                     /locus_tag="ACGI8A_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFH0181556.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            2064..2819
                     /locus_tag="ACGI8A_003593"
     CDS             2064..2819
                     /locus_tag="ACGI8A_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181557.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            2911..3876
                     /locus_tag="ACGI8A_003594"
     CDS             2911..3876
                     /locus_tag="ACGI8A_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0181558.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         1623 a          380 c          672 g         1349 t
ORIGIN      
        1 atttattaaa ttttaatgtt actagaagtt tcaattctag taacattttt tatgttaaaa
       61 acagatatag atatgtattg aaaatgataa ttattattga taaactataa aagtgaaaat
      121 gataataatt ttcattttaa ttaataaaag atttttaagg agggagaatg aagaaaaggt
      181 atttagtcat aggaataata attttgtctt atttttcttt atttatagga gctgaagata
      241 taaatataat gcatatattt gccaaaaatc agcataaact tatgatattt atcatgagta
      301 gaataccaag attaataagt atattgattg ctggtgtagg aatgagcata gcaggtctta
      361 taatgcaaca gataagtaag aataaatttg tatcacctac aacaggtgct acaatagatg
      421 cagcacaatt tgggatagta atatgtatgc ttttagtacc aactgcctct atttttacaa
      481 agacaataat agcatttgta ttttctcttg tagggacttt tacgtttatg aaaataatag
      541 ggaagttaca atttaaaaat ataatctttg taccattagt aggtataatg tttggaaata
      601 taatagggtc tatgacggat tttatcgcgt ataaatacga tttgagtcaa aatgttagtt
      661 cttggatgca aggtgacttt tctatgatac ttaaaggaaa ttatgagata ttatatatca
      721 caatacccct tataatatta gcatatattt atgcaaataa atttactgtt gttggtatgg
      781 gtatggattt tgcgacaaac ttaggtctta gttacaaaag aattgtcaat ataggtctga
      841 taatagtagc acttgttaca gtatgtgtag tcgttacagc tggtaatatc ccttttatag
      901 gtctaatagt tccaaatatc gtatccttgt acatgggtga taatataaga gcaagcatat
      961 ggtatactgg tttacttgga gcgatatttg tacttatatg tgatattttt ggaagaatta
     1021 ttatttatcc atatgaaata tctatagtgc ttacagttgg agttatagga agtatactat
     1081 tcctttattt aatattaagg aggaatgtga atgaagctta gtctcaatca aaaaatttac
     1141 tttgtaagta tattactatt catatcaatg gcacttttta taatttatgg aatagacatg
     1201 aatcatttag aatatgcatt atcacaaaga ataccaaaaa tatttgctat ggtattaggt
     1261 ggaggatgta tagcatttac tactgtagtg tttcaaacaa ttacaaacaa tcaaatatta
     1321 acaccaagtg tactaggttt agattcattg tatgtaatga ttcaaactat aatagtattc
     1381 atatttggtt caagcagtag tctgattata aatgaaaatt ataattttat tattaatgta
     1441 gctatgatga ttggagcttc attgttatta tataagacgt tatttgaaaa aaataaaaat
     1501 aatattttct ttttaatcct agtaggaatg atatttggaa ccttatttaa aagtgcaaca
     1561 acatttattc aggtaatgat agaccctaat gaatttttag ctttacaaac tagtataatg
     1621 gcaagtttaa acaatataaa cactaatgta ttgttaatag catttataat aattattgcc
     1681 ataatacctt ttatatatga tgaaataaag tatttagatg tattgtcgtt aggtaaagaa
     1741 caagcaatta atttaggtgt agattttgat aaagcagtta aaaaaatgat aatattaata
     1801 gcaattttag tctcaatatc aacagcactt attggaccta tgacattttt aggactttta
     1861 cttgctaata taacaagaga aatttttaaa acatataagc atacatactt gatttcgggt
     1921 tctatgttga tagggatgat tacattgata ataggtcaat tttttataca acatgtattt
     1981 aagtttgata caactttaag tgtagtaata aattttatag gaggaattta ttttattcaa
     2041 ttactattaa agggggcaaa tagatgatag agataaaaaa tatatttaaa agatataaga
     2101 ataaaaatgt tgtagatgat gtctctttta gtatagaaaa gggaaagata acatctttta
     2161 taggaccaaa tggagctgga aaaagtacag tactatcaat agttacaaga cttataggag
     2221 gtgatggtgg agaagtaata atagaaggaa aaagcttgac taattatagc aataaagaat
     2281 tagcaaaaaa aatagctatt cttaaacaat ctaacaatat aactttaaag cttacaataa
     2341 gagaattagt tggatttgga agatttccat atagtgaagg taatctaact aaagaggatg
     2401 aaaattatat tgatgaagca atagaatata tgaagttgac tgatatacaa cataagtatt
     2461 tagatgaatt gagtggaggt caaagacaaa gagcttatat agcaatggta atagctcaag
     2521 atacagagta catattactt gatgaaccac tgaacaattt ggatatgaac cattccgtac
     2581 agatgatgaa ggtacttaga ggtctttgtg atgaattaga taagactata gtattagtaa
     2641 tgcatgatat aaattttgca tcatgttatt cagataatat agttgcatta aaaaatggaa
     2701 aagttgaaaa agtaggtcgt acagatgaga tagtaaatga aaaagtttta gaagatattt
     2761 atgagatgaa ttttaacatt aagaatataa atggaaatcg aatctgtatt tatttttaaa
     2821 atattgtata tgaaaataaa cttgctatat gattttttat aaagttttta aattaaaata
     2881 aagattaata ggagagagga aaatattgat atgaataaaa aagcagcaat agtagcagca
     2941 gtagctatta taggattagt gacagtgttt gcattaggtg gaagtaaaaa gaatgaatca
     3001 aaaacatcag aaaattcaaa taatactatg aagataactc ataacttggg agaaacagat
     3061 gtaaaattaa atcctaaaaa agtagtggta tttgattatt cggctttaga tacaatggat
     3121 gcattaggtg tagctgagac tttagtagga cttccaaaag cgagtttacc agcatcctta
     3181 gaaaaatata aggatgacaa gtatgcagat ttaggaggat taaaagaacc agatttagaa
     3241 ggtataaagt cggcaaatcc agatttaatt ataataaatg gaagacaaga agatttttat
     3301 gagcaattgt caaaaatagc accaacaata agtacaagta aagatgataa gaagtactta
     3361 gaatctgtta aaaataatat agacaaaata gcaaaaatat ttggagtaga agaaaaagca
     3421 aatcaagaat ttagtaaaat tgaaaagaaa atagaaactt tgaataaaaa agtaacagat
     3481 aaaaatttaa atgctttaac tataatggta aatgaaggta atttaagtgt atttggtgaa
     3541 gaatcaagat ttagtatatt atacaatagt tttggatttg aaaataaaga taaaaatata
     3601 aaagaatcaa gtcatggaca aaatattact tttgaatata tagctaaaca aaatccagaa
     3661 gttatgttcg ttatagatag aggaatagct actggcagtg atgtaaaaga aagctcaact
     3721 gcaaaatctg ttttgaacaa tgatataata aaatctatgg atgcatataa gaatgataat
     3781 ataatatatt tagattcacc aacatggtat gtaaatgatg gtggtctaac atctttaaat
     3841 aaaatgatag atgatgcttc aaaagcggta aattaataat ccgatataac caaaatgtat
     3901 tttttataaa atgattatca aataaactag gagtttttaa gaactcctag tttatttttt
     3961 gcataaaaaa gatatagttt aaacaataat attatctata ataagataaa tctattcaaa
     4021 atct
//
LOCUS       DAYATN010000217         4014 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.221, whole genome shotgun sequence.
ACCESSION   DAYATN010000217 DAYATN010000000
VERSION     DAYATN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.221"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            295..1770
                     /locus_tag="ACGI8A_003595"
     CDS             295..1770
                     /locus_tag="ACGI8A_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFH0181559.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1857..2270
                     /gene="speD"
                     /locus_tag="ACGI8A_003596"
     CDS             1857..2270
                     /gene="speD"
                     /locus_tag="ACGI8A_003596"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFH0181560.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2300..3151
                     /gene="speE"
                     /locus_tag="ACGI8A_003597"
     CDS             2300..3151
                     /gene="speE"
                     /locus_tag="ACGI8A_003597"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFH0181561.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3141..>4014
                     /gene="speB"
                     /locus_tag="ACGI8A_003598"
     CDS             3141..>4014
                     /gene="speB"
                     /locus_tag="ACGI8A_003598"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFH0181562.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKI"
BASE COUNT         1614 a          433 c          718 g         1249 t
ORIGIN      
        1 aaagcttact aaaactcaaa aaagtaaaaa aaacaaaata ataaaaaata agaaagttta
       61 taaatactaa gttttaaaaa aattaatata aacaacaaaa aaataaaaag tttataaata
      121 ttgatatagc taatgtatat gtttttgagt aacttcaaaa aaacacttgt aaaaatgctg
      181 agatgttaat ataataaata tcatgagttt aataaaagta aaaataaaaa atagaaataa
      241 taaataataa atattaaaaa tatttgaata tagagaggag atgaatttga aatcatggat
      301 agcgagtttg caaatgcagt tgaattaagc tatgagtgtg ctccattatt agagtcgtta
      361 aaggaatact cagagaaaga tatagcttgt tttgatgtgc ctggacatgt aaaaaataga
      421 ggtgtagcta tattaaataa atatctaggt gaaagtctca tgaaaatgga tataaattca
      481 tcaccaacta tggataatgt atctgcgcca aatgggatta taaaaaatgc acaagatttg
      541 ctagcacaag catatatggc tgatgaggca ttctttataa cgaatggcac aactcaagca
      601 atacatgcaa tgattttgag tgttataaaa ccaggtgaaa aagtattact tccaagaaat
      661 atacataaat cagtaataaa tgcgttaata ctttgtggag gaataccaat atttatacaa
      721 ccagaatttg atgaaaaact aggtataagt ttaaatatta ctcttgaaaa agttagaaca
      781 gagatagaaa aagattgtaa tataaaagca ttatttttcc ttaatccaac ttactatgga
      841 gtttgtgcag accttgagag tattatagaa ctatgccata aaaataatgt tttagtgtta
      901 gttgatgaag ctcatggtgc acattttcca tttcatttag atttacctcc ttctgcaata
      961 agtttgggag ctgatatggt agctgtaagt atacataaaa ctggaggagc attgacacaa
     1021 tcttcagcac tcttattgaa tagagataat gtaagttttg aaaaggttct tcaatcaatc
     1081 aatatgctac aatcaacatc agcatcttat ttgcttatgg caagtataga tggtgctaga
     1141 gttaatctag ttgagaatgg tgaaaagcaa ctgtcaaaag cattaaatct atctaggtat
     1201 gcaaaatcta aattaaacaa aattgatgga ataaaagttt tatctacaga aatattgaaa
     1261 caaaaaggtg ttaaatttat agatgaaaca aaattgtgta taaatgtaaa agaattaaat
     1321 ttgactggat ttgaagtata cgatttatta tataaaaatt tttcaattca agtagaattg
     1381 ggagattcat ataatatact ggcacttgtg tccattggaa caaataaatc agatatagat
     1441 agattagtaa aagcactttc tataattgca aagatttata gaaaagaatc aacattaaat
     1501 gaatttaata tggttcaaat aaatccaatt attaaactta atccaagaga agctttttat
     1561 gcaccaaagg aaagtgtaga aatcaattca tgtatagata gaatttgtgg agaatcaata
     1621 atggcatatc caccaggtat accgattatt gctccaggag aattgataac agaagaaatt
     1681 atggagtata ttattttctt aaaaaatagc aatgcatatc ttacagatgt acaagataaa
     1741 aacttagata gaatattagt aataaaataa attactttat tttgcaaggg caagtatttg
     1801 ttaagtagag tttatcaaat agaacttatt aaaagtataa aggagttggg gttgaaatga
     1861 aatttgaaac actaggaaga catatactag tagaatacta taattgtgat gaaaacgtac
     1921 ttaaagaccc acgtcttata gaaaatttta tgaatgattc agcattaaat gcaaaagcga
     1981 caattgtgga ttctgtattc caccatttta atccttgggg ggtttcagga gctgttataa
     2041 ttgctgaatc tcatttgaca atacatacat ggcctgaata tggctatgca gcagcagatt
     2101 tttttacttg tggtgatata gacccatgga aaagttttga attattagag caacttttaa
     2161 agtctgaaag aagcgagtct acagaaattc caagaggctt aactacaaaa attaaaaaat
     2221 attcaaaaaa agatttaggt aagattactc ataaaccaga agcagtataa aacatacaag
     2281 agaaagtgag gattattata tggaactttg gtatacagaa gaatggacag aaaatgtaag
     2341 attttctata aaagtaaata aacatctatt tgaaggaaag tctcaatttc aaaggataga
     2401 tgtgtttgat agtgatgaat ttggaaaatt tctaacaata gatggattaa tgatggtaac
     2461 atataaagat gaatttatat accatgagat gataactcat gttcctatgg ctacaaattt
     2521 aaacataaag aaagtattag tcataggtgg aggtgatgga ggtacagtaa gagaattaag
     2581 ccgttatcct caaatagaaa agattgacat ggttgaaata gataaaatgg ttgtagatgt
     2641 atctaaggag tatatggata tatgctcatg taaattagat gataaaagag tgagtttata
     2701 ttttgaagat ggtgttaatt ttgttaagtg tgcacatgat aaatcatatg acctgataat
     2761 agtagattca actgacccaa taggaccagg tgaagggctt ttctcaactg atttttataa
     2821 ggattgttat agaatactga ctgatgatgg aattttagta aatcaaagtg aaagtccgta
     2881 ttttgacttt aatgctaaag agatgaaaag agctaataaa aaattaaaac agatatttcc
     2941 aatatcagaa gtttatcaag cacatatacc aacatatcca tctggtcact ggttgtttgg
     3001 atttgcttca aaaaaactta atccagtaaa aaatcaagat agaaatggat gggagaaatt
     3061 aagtttaaaa actaaatatt ataatagtga tatacattta ggttcgttta tgttacctca
     3121 atacgtgaag gagatgctag atgaagaata atttctatca tatgaacact tttatgagta
     3181 tggacaaaaa ttatgaagaa tctaatctta tagtatttgg tgttggattt gatggaacta
     3241 cttctaacag acctggggct agatttgcaa gtagctctat gagaaaagaa ttttacggtc
     3301 ttgagacata tagtcctttt ttagatttgg atttagagga ttataatata tgtgattatg
     3361 gagatttaga aattagtgtt ggaagtacag aacaagtctt aaaagaaatc tatcaagaga
     3421 catataagat tgttagagat tcaaaggtac cctttatgat tggaggagag catttagtta
     3481 cattaccagc ctttaaagca gtacatgaaa agtacaatga tatatatgta attcattttg
     3541 atgcccatac tgatttgagg gaagaatata ataatagtaa aaattctcat gcaacagtaa
     3601 ttaaaagaat atgggatatt gtaggtgata ataaaatatt tcaatttggt ataaggtctg
     3661 ggacaaaaga agaatttaaa tttgctacag aagaaaaaca cacatacatg gaaataggag
     3721 gaatagatac gtttgaaaat atagttaata tgctaaatgg aaagaatatt tatctaacta
     3781 tagatttaga tgtattggat gcatctgtgt ttccaggaac aggtacacca gaacctggtg
     3841 gtgtaaatta tagagagttt caagagattt ttaagattat aaaaaactct aatataaata
     3901 tagttggttg tgacattgta gagttaagcc ctgattacga tacaacaggt gtatcgacag
     3961 ttatagcttg taaaatctta agagagttat gcttaataat atctgataaa atta
//
LOCUS       DAYATN010000218         3981 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.222, whole genome shotgun sequence.
ACCESSION   DAYATN010000218 DAYATN010000000
VERSION     DAYATN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.222"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            32..904
                     /locus_tag="ACGI8A_003599"
     CDS             32..904
                     /locus_tag="ACGI8A_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181563.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            901..2190
                     /locus_tag="ACGI8A_003600"
     CDS             901..2190
                     /locus_tag="ACGI8A_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181564.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2120..2764
                     /locus_tag="ACGI8A_003601"
     CDS             2120..2764
                     /locus_tag="ACGI8A_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181565.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMVPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3031..3495)
                     /locus_tag="ACGI8A_003602"
     CDS             complement(3031..3495)
                     /locus_tag="ACGI8A_003602"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0181566.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
BASE COUNT         1493 a          468 c          604 g         1416 t
ORIGIN      
        1 tataaaattg tttctggggg tgtatttata tatggaaatg aaaatagcag tatccaatct
       61 tagtaaaaga tttgggaaaa aacaagctct tgataatata aatcttgaaa tcgacagtgg
      121 tatgtttggt cttttaggaa gaaatggagc aggaaaaacc acatttatga gaattttagc
      181 cactttatta tcaaaaacag aaggttcaat acatatgtgt ggagttccta ttgagaatac
      241 taaggaaatt agaaatatgg ttggttattt gccacaagat ttttcaatgt atcctaacat
      301 gacagtatat aaagcaatgg attatttagc agttttatcg gatataccac taagaaaaag
      361 aagaaaaatt atagcaaagt tattaagcaa agtaaacctt actaattatc aaaatgtgaa
      421 agtaaaatct ttatctggag gtatgaatag aaggcttggg atagcacaag cattaattca
      481 taatcccaga gttttaatag tagatgagcc gacagcaggt cttgacccag aagaacgtat
      541 aagatttaga aatttactat gcgaaattgc aaatgataga atcgttattt tatctacaca
      601 tattgttgga gatatagaag ctacatgtga aaacgtagct atattgaata gtggaaaagt
      661 catatacaat ggctcaacag aagcattagc aaaactagct gaagggaaag tatatagtat
      721 tgaagtagat aaaaaagaca ttgaaaatat aaaaagtaga tttatagtta taggaatgtt
      781 gactcatggt ggaaaggcta tacttaggat tatatcagat gataaacctt ttgagacagc
      841 agtaaattgt aatccaacta ttgaagatgg ctatatgctt ataatgggag gagataatat
      901 atgaatctat ttataaaaga atgtaaaaat atattaaaga gtatggtata ttatattttt
      961 attgtagttt tagttttttt ctatatttct caacttggaa gttcagtttc aaatgatgta
     1021 agtaaaaata ttcctaatga tggaaatcca ttagttgctc cacaaaaagt agagaatatt
     1081 gaggaatatt attctgtaca caatcaatat ccatatggtc aaaaagaatc tatgaaaaca
     1141 gaaaaggtca ttccaaatgc aactataaac ctaattagag aatatcaaaa aaacacatat
     1201 acaacgtatc caacaggggt agttaagtat ataaaattag accaaaataa aacgaagacc
     1261 atagaaaaat atatagaaga aataactggt aaaagtatta gcgaaataaa ttcactttgg
     1321 aaaactgatg aaagtataaa aatagattat tctaaagtat ctcttgaaag atttgaagaa
     1381 ataatgcaaa gagtagacga cattttagga ggagggtctg catatgcttt tgacaagtta
     1441 caaagatttg gagtagttcc agttacctat gatgaagcta tgaagaatta caactataca
     1501 atacaaaaag ataaagttag taatgggtat gcaagaattt tttgcgatta tataggtata
     1561 gttttaagta tatttccagt atttgtggca gtttttatgt cacttaaaga taagcgttca
     1621 agaatgagag agattattta ttcaaaacaa gtatcttcat gcaaaatagt tttatcaaga
     1681 tattttgcac ttgtctttat gatgatattg cctgtattaa ttttaggtat gaaagattta
     1741 cttcctttga ttttctttgc taaagataaa ggtatatcta ttgataactt agcatttata
     1801 aaatatacat tatggtggat actaccaact cttatgatag ttacagctgt aggaatgagt
     1861 attactatat taacagatac accagtagca attattgtgc aattaatttg gtggctttac
     1921 agtataaatt ctacaggact caaaggtgat tattcaacaa tggggctaat gataagacat
     1981 aatagtgtat taaatgggga tattattata caaaacttca atcaaattat gattaataga
     2041 attgtgataa gcttgggagc attggtgctt gttttgttag ctatattctt atatgagcaa
     2101 aaaagaaggg ggaatcttga tgtttgggca aagttttcaa aactgtttag ttctaataaa
     2161 agaaaacttc aaattaagca ctttaactaa tttaatatgt tcaattatat tgctatgtat
     2221 ggttcctcta atacaaggag tagctaattt agacagaatt tcaacagcta tatgtttaga
     2281 aaattatgtt gcaataatag ggatagtaat gttggtacct gtattttcgc tagagcaatc
     2341 aaaagaaatt gatgagatag tatcttcaaa agcattatct caagttattg tacaattaat
     2401 taggatactg atgtcaatta taggtatagt ggtattaatc ataggttttg taaatgtttt
     2461 aatatatctt ggatgtaatt ttccagtagt tgagtatatt attggaagtt tcgcatcagc
     2521 tatgtttata ggaagtttag gaatgttttt ctcaaattta tttaataata caatcatagg
     2581 ttatatgata tctataggat atttaatttt aaatatgatg actaaaggca agtatgttgg
     2641 aaaatttttt ataatgtcta tgagtcaagg tagttttgat gagaaatatt ggctgatact
     2701 tggaagtgtt gtgttgataa ttgcatctct cttaataaaa ccagtaaaaa ggaagattag
     2761 ataataaaat gagagcttag ataaaataaa ggtaatacaa ataaaaagta catttatgaa
     2821 atataaatct atttttatat acacaaacct acttacaatc tctttatata tgtagataga
     2881 tttatatatc cgtagtataa aatatatatg tatacaaaat tttatcaaaa gctctcaaat
     2941 ttatatttca aagacactta ttgctatgac ctacacatat gtattttttc attttaattg
     3001 ttgtattaac aataagacta ttgttataaa ctattgatac ctcataacaa aaaattctcc
     3061 atctcctaaa tgcgtttttt gtaccttagt ctcaactatt ttaaatcctt gagactcata
     3121 acattttaca gctctcatgt tccaggttct tacatttaat acaattggtt tagtactata
     3181 tttatttcta cattcttgta atcctaggcc aattatttct ttaccaattc ccattccaca
     3241 ataattcggt ttcataccaa taccaaagaa tacagagctt ccttcatcta ataagtttat
     3301 aaaacctatc aattctttat tgtcatttat aaaacttata aaattttttc ttttatcttc
     3361 ttttgctagt gaaaaattct tctttttaat ttcctcccat ggaggtagat tgtatattga
     3421 atatttacct tcatatttcc aattagtaac ttcaatagca tgttcttcag taaatttaac
     3481 tttatttaaa ttcatatgtc ctcctattaa aatatttaag gtaattaatg gtattttaaa
     3541 gttttagaaa ataagtaaaa ctatttatat ttatccataa aatctttaaa aattttacta
     3601 acaattcttg taccctgtac tttttattgg attgacagaa gactataaac taattttgaa
     3661 ttaccttcta attcaaaaac atcacgatat atatataatt tcttagcttg attaaattct
     3721 gttaaatccg tttcttatag ttgtataatc aacatcaata tctttatgtg tattagttaa
     3781 tttattggtt ttaggaactt attattaata tattgtattt gctccaataa tccaagtagt
     3841 aggtaaatta taggcaaatt atcaaatgta tatctatcca tgcttaaact ttagagatac
     3901 aataactact aaattactat tctagtagtt acaaaaaaga atctttaaaa atattaattt
     3961 aacttatatt ttttaataaa t
//
LOCUS       DAYATN010000219         3964 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.223, whole genome shotgun sequence.
ACCESSION   DAYATN010000219 DAYATN010000000
VERSION     DAYATN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.223"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(225..605)
                     /locus_tag="ACGI8A_003603"
     CDS             complement(225..605)
                     /locus_tag="ACGI8A_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0181567.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(806..1999)
                     /locus_tag="ACGI8A_003604"
     CDS             complement(806..1999)
                     /locus_tag="ACGI8A_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFH0181568.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(2062..3318)
                     /locus_tag="ACGI8A_003605"
     CDS             complement(2062..3318)
                     /locus_tag="ACGI8A_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFH0181569.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNACSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     regulatory      complement(3583..3659)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT         1439 a          730 c          415 g         1380 t
ORIGIN      
        1 aagaatagaa acaagttcta gattactata gagatgacat atcaaaatat ttttaaaaag
       61 taatttaagt attagaagta attgaagtta gtaaatataa atgcgtttac ttttaagtat
      121 tgcttaaggg aataaaaaat aaaaataaac tctgcaacca tctaaaaata gttgcagagc
      181 ttacaaaacc aaaattatct aaattgtaat aaaaataata acatttatac ataagcttct
      241 tcccaattta ctacccagtt ttctaacgac tttatctttt cctctgttag ctcctcttcg
      301 tgaaatgata ctaataaatt ccttataaaa ttattcttca aaagattttg cacatttctt
      361 gatataaact ctaaatatgc cttttctttt attgcaactg tataatacat acacttgcct
      421 gtttcttgaa catataagaa tcttttcttt gatagttttg ataaagtttt taaagctact
      481 ttttctggta aattatgttc ttctttcata taatcaataa tctcatatga attggttttt
      541 atatctaaat tccaaataaa tttcataata actaactcta attttgataa tttttttaaa
      601 cacatagtta tcactccgct tctttatcta tataattaaa tattacaata ataatttcac
      661 aaaatcaatt ctaataatca taacatacct taaaatctgt tcttaacttc taccttctta
      721 atctcccaaa tgattttttt atattgtatt caatagtata atccttttcc aaaatataac
      781 ttaccagtta aaataatctt tatttttact tactctcgcc ttttacagaa gttaataatt
      841 ttttataagt tacatcaatt ccaaaagctc caagaggtgc agttataagt atagctagaa
      901 ctgctaacgt caaaataagt tgtccagatg ccatccccat tgcaagtggt acacctccta
      961 ttgctgcctg tactgtagcc tttggaatat agccaatcat acagaacaac ctttccttta
     1021 ttgtcaaatt tgtcttaata aggcagaaga atactcctac catacggaat aaaagaactc
     1081 caaatattaa aattacagct ccaattcctg ctttaaatgc ataacttata tctactgttg
     1141 cccctaccaa aacaaacaag agaatttcag cgccaaccca tagttttgaa tactttatgg
     1201 acaatcttct tgcaactaca cgatatttta tctgaaggaa aatcccaata ctcataactg
     1261 ctaataatcc agaaaatggc acaataccct ctaaagcagt ctctaaactt atcattaaaa
     1321 atgagatact taaaagcaac acccctttag cagaatctct catatgaacc ttcttaaaaa
     1381 atactgctaa acagattcca atcactgctc caactattac acctaatata attgatactg
     1441 gaatttgtaa aaaactaata gctgatatac tatttccttg tgccaatcct gtgaatactg
     1501 taaacatcac aattacgaaa atatcgtcta tagaagttcc tgctaaaagc atctgtggaa
     1561 tacttttttc tgtaccataa ccttcttcca ttaatttaag cattttaggt acaataattg
     1621 cgggagaaac tgctcctact actgcaccca tgactgctgc ttccaatata gaaacgccta
     1681 gtaattttgg tgctaaaact atcattccga taatttcaaa agtagctgga ataaagcaca
     1741 taagtacagc aggtcttccc actttcttca aatcgtttat atctaaagat aatccagccc
     1801 ttgttaatat aataatcaat gcaatctttc taaggtcaga agaaatatct agtattgaat
     1861 tatcaatcaa atttaaaaca tatggaccta atataattcc tgtaagtaac attcccaata
     1921 agttaggtaa ctttatcctc ttaaaaatac ctcccgaggc cattcctaat aaaaaaatca
     1981 aagctaaact tgtcaacata ttcctaccct atcctttctc taatattctt tctcttctaa
     2041 tcatacatta tgttgtttta tttattgttt tgttgaaatt ttttgtagtg aataaataga
     2101 tttatccata ttaaatatat atgaaatagc gcatactaca aagaaataag gtaaatagct
     2161 gtatccaaaa acttctgccc ctataaatac aggtgcaaaa aaagtatttg tagcactacc
     2221 aaagacacta gcatagccaa gcgctgcaac taattcaatt ggcaaattaa aaaatcctgc
     2281 taacaataca ccaagactag ctccaatgga aaacaaaggt gtaacctctc ctccttgaaa
     2341 acctgctgac aaagtcagta ttgtaagtat aaactttaac agccaatcaa atgaataaat
     2401 ttctccacca tagaaactat tttgaattaa atttgtccct aaacctgaat atcttccttt
     2461 ataaaataaa aggaataata cactaagaca tactccaata atagcaattc ttatcatagg
     2521 attttttaat ctattaccta tttttctttt tgacaatttc agacaccatg caaatgctcc
     2581 accaaccatt ccaaaaataa ttcctaaaac aataagcttc caaaatattg acaagtcaaa
     2641 tacaacttta gaagaaagtg caaaagaaaa tttctctaac cctaaagctt ttgatactga
     2701 acttgctgta aaagaagcag taaacgcagg aaaaagcgat tgatactcca gtgaaccagc
     2761 taccaaaacc tccatagcaa atagtattgc tgctattggt gtctcaaata atcctgcaaa
     2821 accagctgcc ataccagtta ctagaaaaat actacaagca ttttttatag gcaacctttt
     2881 acctacccaa tgtgaaaaag ttgcaccaat ctgaacagca actccttctc gtcctgcact
     2941 accaccaaat aaatgggtta accatgtacc tgatattata aatggaacca atcttaaagg
     3001 aataatttct tcttctccat gccctacctc aaatattaga ttcattcctt tactactttt
     3061 tcctccaaat ttaaaatagc aataagcaat aacaactcct actactgcta aaaatggtat
     3121 cagatacatt ggatatgttt ctcgaatatc tgtaactttt agaagaaccg ttccaaatat
     3181 ggtgtctata agacctataa ttgcaccaac tggtatacct atgagtccta aaaaaaatag
     3241 tccaccatat gttttataaa cgcttttaat tcttattatc attttaatcc atattaaagt
     3301 tttaaatttt tctatcattt taattccttt ctatttgcat cataagattt tttgaaagtt
     3361 ttattacatc aactgtagtc aatgtaatga ctatcataat tgttttttaa attgatagga
     3421 tataggtaca ctcttaaccc attcttaata aaataattaa catataatct actatttttt
     3481 ctattaatta aatatttctt caaatttaaa aaataaaaat ttagtaagta cttcacctcc
     3541 attttattga tttaaacaac aaaaaaagct ggtggcatct atgatatcat agaagtcatc
     3601 agctcattgg cagtttagga tattatcatc caagggagaa cctcattccc ataattatta
     3661 tataattttt tatttttcat atgtctattt tatgtaaatt gtatttttaa tgttataaat
     3721 aatataacct atcaattaaa atagtaaagc ctctaatatt atgagtgttc aatatttcat
     3781 attattttta tgatatataa tttaaatgat tttctttact ttgcattatg atacataact
     3841 taaatatttt tcctcactcc acattatgat atattattta aatatttttc ttcactctgc
     3901 ttatattatg atacatgatt taaatatttt cttcactctg cttatattat gatacatgat
     3961 ttaa
//
LOCUS       DAYATN010000220         3958 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.224, whole genome shotgun sequence.
ACCESSION   DAYATN010000220 DAYATN010000000
VERSION     DAYATN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.224"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(519..1838)
                     /locus_tag="ACGI8A_003606"
     CDS             complement(519..1838)
                     /locus_tag="ACGI8A_003606"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0181570.1"
                     /translation="MLNINVFWIFLPIFSTAIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADK
                     YIKDINKQIKECNTIFNTQNMILDVILSDKKSICDMNNIYFLVDINFLECNFIEMPDI
                     CSIFSNIIDNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKVI
                     TSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
     gene            complement(1832..1975)
                     /locus_tag="ACGI8A_003607"
                     /pseudo
     CDS             complement(1832..1975)
                     /locus_tag="ACGI8A_003607"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
     gene            complement(2099..2809)
                     /locus_tag="ACGI8A_003608"
     CDS             complement(2099..2809)
                     /locus_tag="ACGI8A_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFH0181571.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKKTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
BASE COUNT         1506 a          558 c          282 g         1612 t
ORIGIN      
        1 ctataaataa ttaaatcttc tacattaaaa atcatttctt cagaatctat cacaactaag
       61 tcctcctcaa attagtaaaa tatttttctt taatatttat tactaatatc aactttacta
      121 tctaattagt tatttaattg ttgtttttat gttcctagtt ataaagttta tcaaatttac
      181 attttatttt tttatactat atagaacttt tggtcaataa tgttaccata ctaaggtcgc
      241 atttgcagat aaaaataact ctatttttct aaatttattt aagatatatc atgcaaataa
      301 tataactata aaacctatca acattttaaa ttcaaacaaa tataaatcac attttttgtt
      361 ctattatttt attgagtact aaaaaggatg ttatcttatt agcatagcat tcaaccattt
      421 ttatcacctc caaatatcct atgccttaaa gatagcatct tctattttat ctttcaatac
      481 tactgctcca actgggcaat ttagagcccc aactagaatt agttcctagt taatggtata
      541 taaatagtaa ttgtaaactt gtttttatct gagtatattt ccacatttcc attatatttt
      601 tctactgaac tttttacact acttattcct attccatgta aaaaggaatc tttcttactt
      661 gtaataacct tattattttt taatacaact tcatttatct ttgtattttc acattttatt
      721 acaaataacc tatttataac agttcctctt aatttaatct ttttttgtat acttgtgtcc
      781 ttaatcttaa tacaagcttc tattgcatta tctattatat ttgaaaatat actacatata
      841 tctggcatct ctataaaatt acattctaaa aaattaatat ctactagaaa atatatatta
      901 ttcatatcac aaatagattt tttatcactt aaaataacat ctagaatcat attctgagta
      961 ttaaatatag tattacattc ttttatttgc ttatttatat ctttaatata cttatctgca
     1021 aactcatttt tcccataaac attctgaata caaatgatat gattattcat atcatgatat
     1081 agttttctta ttttcaactg atattcctgt aaatttaaat aatatttata ttgcatattc
     1141 attctttttt ttgtaatttc atattctatc tttaaactat tatcttttat tattcttcct
     1201 attatgctta ctaatgaaag atttgatagt aaaaatacag ttgatattgt aagtattatt
     1261 aaactctctt tagaatcgat aatcttatct ttaaaaataa attcaaatat tactattaca
     1321 ccaataatgt ttgctattaa tggaatagat aaatatatac aatccctctt gtttattttt
     1381 gtttttaatc tgataaactt aagcaatggt attaatacta ttagtaatga tttagatatc
     1441 attattaact ctaacttcaa caaattatcc tctaataaat cattcatatt ttttatagaa
     1501 ttaagtgtca ttactatact taatccaata ctatcaaatc caattaataa catccaatat
     1561 agcaaactta taaatacact tttccattta tccactacat aatttatttt atacaataca
     1621 tatgtaataa atatacatat aaataattta atatttatat taaactcaat tgtattcaac
     1681 atagtaatta tcataatagc aattactatg ctactattta gggttaatct attctttctt
     1741 aactcactaa attcatttaa taaaaatttt aatacaatcc attctatcgc cgtagaaaat
     1801 ataggtaaaa aaatccaaaa tacatttata tttaacataa tacatctccc aatacatatg
     1861 ttaatttttt ctttaaatta gccattctat atttactaac tggtatatct ttaccattaa
     1921 taactacagt atttttacat ataaattgaa tatactccaa attaattaaa tagctcttgt
     1981 aacattttta aaaatttatt ctttcttaaa tcactgaatt catttgataa aaattttaat
     2041 acaattcatt ctatcgccgt agaaaatata ggtaaaaaaa tccaaaatac atttatatct
     2101 aacataatac atctcccaat acatatgtta attttttctt taaatcagcc attctatatt
     2161 tactaactgg tacatcttca tcattaataa ccacagtctt tttacatata aattgaatat
     2221 actccaaatt aattaaatag ctcttatgac atctaaaaaa attatatttt tctagctcta
     2281 attctatttt atccatacta tttttagtat aataagtatc atttttagta tgtatagtca
     2341 aaaccttttt cctaacctct atatacataa tactattaat taaaatttta ttgattatac
     2401 ctttattttc tatcagcata aaattttctc ttttttttat aatatccgat atacaactca
     2461 aaatactttc atttaaatct ccaaaatcta taggctttaa taaatatcta taagccctta
     2521 ccttatagcc ttcctgtatg taatcaacta ttgaagttat aaatattatt tcagaagtat
     2581 cattagtttc tctaattatt ttagccacat ccatgcctgt tagttgaagc attttaatat
     2641 ctaaaaaaaa tatatcaata ccttctaaat ttgtttccaa taattcttct cctgaattaa
     2701 actcaaatag ttcaatttga ctagaaatac cctctaagga ctttaatata cactctctta
     2761 aaaaagctat ttgtgtaata tcatcctcac agataactat tctatacata aacacccctc
     2821 cttagacatt agatctgaaa acaaataaaa tatcaattta taaataatta tttagttata
     2881 caactaacat ggtcatcaaa tactattatt cacttaaaaa atcaccccct atttttagta
     2941 tgttaacaac aaaataagaa gatatcttag atttttaaaa acaattaata cttaatgtag
     3001 ttttgtatac tatttatagg taatttttta tatcatttta aacattaaaa ttattgatgt
     3061 ttaagaacta ttatattgat agtaatatat aattaatgat atcaaaaatt tttaattaga
     3121 cttctctctt attcaagatt tttctatata aaatctaaga ataaatagaa aactaacatc
     3181 taacctttta ttttttattt tcgatctatt tacttgaatt ttaacataaa tctatctttt
     3241 agtatatatt aatcatgctg aatttgtgat gacatgtata caaaatatcg tttagtattt
     3301 tctaataaaa aatattactg aaattaaaaa tctacaatag catttaacaa gtaaaaacat
     3361 gtttacacaa atatataatt taaggtcttt aaaatagagt atgtattatt ctaaaaatta
     3421 gttatagtta tcacataaaa atagaaaaga attttcaaaa gatttaaaga acatatatac
     3481 ttcaattgca gaagatgtaa tgttaagtga actagcattc ttcgaggaaa aatagactca
     3541 aaaaattaga aaatagaatc aggttttaga tgatttaagt atttattttc aaaaaagaaa
     3601 tttgattcta agttaataaa gaaatattta aaaaaatcaa aaaattttct taaataagta
     3661 ataggttttg taaaaatgga aaaatatcaa aataagacta tgatttttaa aactatagcc
     3721 ttatcaataa atttttattt aaagaacaca cataaatatt tttaaaccta atttatgtga
     3781 agttttatgt attttttata taagtagtat tctaatctta ctcatctaat ataccaaaaa
     3841 gactgtaagt aagttcactt ttatgcaaac ttacccacaa tctcttttac acattaattc
     3901 tatttattaa aattaaaaat atttactgct attctaccca ataaagatga tactgttg
//
LOCUS       DAYATN010000221         3875 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.225, whole genome shotgun sequence.
ACCESSION   DAYATN010000221 DAYATN010000000
VERSION     DAYATN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.225"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(22..183)
                     /locus_tag="ACGI8A_003609"
     CDS             complement(22..183)
                     /locus_tag="ACGI8A_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181572.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(524..964)
                     /locus_tag="ACGI8A_003610"
     CDS             complement(524..964)
                     /locus_tag="ACGI8A_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0181573.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1036..1506)
                     /locus_tag="ACGI8A_003611"
     CDS             complement(1036..1506)
                     /locus_tag="ACGI8A_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFH0181574.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            complement(1523..2833)
                     /locus_tag="ACGI8A_003612"
     CDS             complement(1523..2833)
                     /locus_tag="ACGI8A_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFH0181575.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAIPLEL
                     DWGIDKDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDKDLVESSLIYWAAGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            complement(2834..3019)
                     /locus_tag="ACGI8A_003613"
     CDS             complement(2834..3019)
                     /locus_tag="ACGI8A_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181576.1"
                     /translation="MIILSKTLSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENDLY
                     SFSEVEDRINKFMKGVS"
     gene            complement(3003..3440)
                     /locus_tag="ACGI8A_003614"
     CDS             complement(3003..3440)
                     /locus_tag="ACGI8A_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFH0181577.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYLSGRTLKKNSFDVHYFPKSNDKSFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            complement(3433..3858)
                     /locus_tag="ACGI8A_003615"
     CDS             complement(3433..3858)
                     /locus_tag="ACGI8A_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFH0181578.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTTYASYVEYGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
BASE COUNT         1160 a          809 c          380 g         1526 t
ORIGIN      
        1 gaagttcata attaaatgaa tctatttgaa cttatggaac ttgattttaa aatcaaattc
       61 ccagccactt ttactcttgc cacgagttga gtggcttttt acttttagaa atactttaca
      121 aattaagcaa aatattaaac tagcaataac gccagctatt acattaagta aaaagttatt
      181 catacttccc acctcctttc attaggaagt aggttttatc tcagtatgaa ctccactcta
      241 taaattgtag attacatctt cttgctaaaa atattataac atataattat tacatatttt
      301 acctattcta tatttatttt tttattttgc tatcttcttc gtcccctctt tctctctctt
      361 tcagcttctt tcattgcttc ctcttcatct tctatcttta taagtattga ggcggctgct
      421 aacgctctct cattaacttc taaattcata tattcacttg gcttccactt taatttttga
      481 atacaataat gagtgatgct agcatcaaaa tcgccacctc tgattagttt tttgcttctt
      541 ctactttatc ctcaaaagat gtatcaaatc cattgacttc attcactttt actgtataat
      601 tgacatactc acctgctgta agcattgtct ttaataactg agcttctccc attactccat
      661 aactattttg gagttcggca tcctttaaat ctggaaatac tgtagatgct acacataatt
      721 cagctacata actattgtag tcaatttcac ttgtatattg tccagtatgc ttaccattgt
      781 taccaatcac ttttactctt ttagtacact ttcttcttag tgcttcgtct tcttcagatg
      841 ataaaacttt taattcccat tcaactggtt tcccttcttt atctaaaaat ctgttactcg
      901 ctacatattt tacattatca acctttattg cattttgact taaaaaagca cttaaattac
      961 tcatattatt ctaatctcct tttattttaa tttttcatat aaaaaataca catatataat
     1021 ttataaatgt gtattttact ccattcctgc caataaatta aatttttcta ctaattccca
     1081 atcctcaaaa gtgaaatcca tatcttcatc taaatactca ccatcagcat caaatttagt
     1141 aattattcca ctgtccatat tacaatcttt aagtactact gtctgtcttc ctacagcaga
     1201 tgtagggtct tcatttgtaa cttgtatgtc aaaataaata tcctcaccag tttctttata
     1261 tctgtaaagt aattctctaa aaatagaagt attataatga aatgttgcac ttccagtatt
     1321 tgtactccca gttgttttat ttccctttgt tgttcttcct agaattggaa cttcactttt
     1381 atttttttcc attttagcct ctaaatctat agcttgcata aaattatatc ttttgccttc
     1441 tatagttata aaacattcag ctttctttgc actaactgta tctttagcat ttattgtttg
     1501 agccatatta tcactcccct ctctaactaa ctgaaactgt catataaagc ttactcatag
     1561 catttataac cttaacagca tcagatacta tgacagtttt cttatcattt ccaagctcta
     1621 cactaacatc atcagtttta aaatcttcta ttgcccttat attctctaat tctttatggt
     1681 gtttaacaac atcattccag aaacttattc ttcctgcctt atcattcgga actttaccta
     1741 aatacttttc attaaataaa gttgcaatat cattagcaat ttggtcaagt actctaacac
     1801 tttggttact tgaaaaatcg tcatttttat catctgtaaa tgatacaaaa gtatttatat
     1861 cctctaacac atgaacttca tctcccacct tgtggaatat aaatttacca gtttttaaag
     1921 cttcttcaag ttgtatttgt gtataattaa catcaacatc aaactcacca tcatactttt
     1981 tattagtatt agatttattt atatcgcatc ctgctatagc tccagccgcc caataaatta
     2041 aactagattc aactaaatct ttatctttaa tcttattttc tacagacact acaccttcat
     2101 aatctgcatc acttttctta tatagtactg tttgaaactt agctcctacc ttatctctca
     2161 ttctctttgt aaattctaca aataaacttt taatttctgt tgttgtagcc aaacatccta
     2221 aagcattaaa gctatagctt tctattttat ccaagaaagc ttggtactct gctcctgtca
     2281 cagcttcgcc attagttcca ccagtaaata caagtcctgc acttgcttct agtgttgcat
     2341 ccttcttcca agtgatatag tcattgtctt gtaagtctgt aataaccttt gctatttgag
     2401 tatctacctt cttattatct aaaagtgtta caacatcaaa tttagtgtta tcatctatat
     2461 ttgttgtaac tgttactttt aagtcatttc ctctgatacc actatatttt gctgtggcta
     2521 tagtacaact ggctttaacg cctttattta atttataaaa atatcccaac cttatatttt
     2581 tgaataaatc tctcaaacct ttcagcttct catgagtata atcatatcca aaatacttca
     2641 ctgaatactt ctcaaaatca tcactggtta cttgaaatac gtctttatct atgccccaat
     2701 ctaactctaa aggtattgca acaatacctc tatccgataa tgaactggtt gcccttgtag
     2761 cacttacaaa atttatatat gcaccaggta ggaccttatt ttgtgttaca aatgttcctc
     2821 cacctaaagc catctaactc actcctttca taaatttatt tattctatcc tctacctctg
     2881 agaaggaata taaatcattt tcttttaaaa ttgcatttaa taaatctttt ctatttacat
     2941 acttcttaga gttaactatc tgctccttag taaacttgta gttatcttct ttgcttaatg
     3001 ttttactcaa aattatcacc tctcttcaaa ccaccgaata actctactgt atccatctta
     3061 tctgtgtcat tactttttat agtaaagtag ttataatcaa caaagaaatg aagaacatta
     3121 tctataattt caaagttcat atttgtacct ctgactaagt ctccatcaat ttctatatac
     3181 tctaattcct ccagtaacat ctcagctatc tcatttattt caaatgattt atcattactt
     3241 ttaggaaaat aatgtacatc aaaagagttc ttttttaatg tcctgccact tagataggat
     3301 actttgcttg gatttaaagg aacaataaaa aaacaaggtt catttatacc ttgctccaca
     3361 tcttcactat aaattgtata actctctcca aatgttttat ctaatttaat agatattcca
     3421 tcaattatat tattaagcat caaatactcc tttaagcaat attaatagtt ttttctctat
     3481 aatcttatca acttggcttt gtagttccat ctctgaaatt gttaagaaat gttgtccttt
     3541 aacccaacct tttccatctt tagttctatg gccatattca acataacttg catatgtggt
     3601 cggattaaca acctctataa tataattatt tccttgttta tacacaggaa gcgacctagc
     3661 ataagccact ccattccatc cttgtcgtaa gaatcctgta tcaactggtg ttcttctaat
     3721 tactttccca agtaatcgtg ctgctaattc tcttgctgca tccttgcaaa acttatctaa
     3781 atcaatcttt gtaagctcct ccatcttttt acaaactctt ttaaactctc taaaatcaac
     3841 actgccccat ctagccatta tgctttatcc ttaaa
//
LOCUS       DAYATN010000222         3870 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.226, whole genome shotgun sequence.
ACCESSION   DAYATN010000222 DAYATN010000000
VERSION     DAYATN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.226"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            176..1057
                     /locus_tag="ACGI8A_003616"
     CDS             176..1057
                     /locus_tag="ACGI8A_003616"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0181579.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1118..2326)
                     /gene="tyrS"
                     /locus_tag="ACGI8A_003617"
     CDS             complement(1118..2326)
                     /gene="tyrS"
                     /locus_tag="ACGI8A_003617"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFH0181580.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2399..2651)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2803..3771)
                     /locus_tag="ACGI8A_003618"
     CDS             complement(2803..3771)
                     /locus_tag="ACGI8A_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HFH0181581.1"
                     /translation="MNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDFIVPVFFC
                     IICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINKPTMVMDD
                     LVYGSVFGLIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLMIALFPFG
                     MVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICVSYLAIFE
                     VLKLLNVKVDSVYLKTIFISITYISLYFIIRDSCMSLQNFIIAKFRKLLYARILFLII
                     ILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
BASE COUNT         1383 a          620 c          434 g         1433 t
ORIGIN      
        1 tttttttata gatattttta atatatatga tactttaata ttatgcaaat aatgtcatat
       61 aagttacact ttagttatct tagttacaac tttgtcggtt tactttttct actttcttcg
      121 atataattat aatatatttt tgatacaatt cgacaaaagg gggaatgctg taattatggt
      181 aaaaaaaaga aaaaagaaaa ctgttacact tactatactt gttgtaatag ttattggtat
      241 tttttctatg gcatgggcgg acatgtctag aaaaaaagaa atgaaagaaa ctcaagaaag
      301 acttaaagct gaaagattag aaaaagaaag aattgaaaaa gaaaaaaatg aacctattat
      361 tggtgcaaaa aaagaagctg taaaagaata tggatatgac gcaaaaatag tttgggacaa
      421 acttaacaaa tacgactatt caaacaatgg agaaaagata gtctttctta catttgatga
      481 tggaccatct actactaata ctccacaagt tctagatatc ttgaaaagac atggtgttag
      541 aggaactttc tttattaagg gtgattctct tgaaagaaaa ggagctaatg aaattttaaa
      601 aagaacattt gacgaaggaa atgcaatagc ccatcactct tatactcatg actataaaaa
      661 actatatcct aacagaagcc taaatctcga tacattcgta aatgaactta acaaaactga
      721 tgaagctatg aaaaaagtct taggaaagaa tttttcctca aatgtagtta gatgcccagg
      781 cgggtatatg tcatggaaaa atatggaacc tctaggaaac tacttaaaag agaaaaacat
      841 ggcatcaatt gattggaacg ctttaaatgc tgatgcagaa ggtaaaaaga aaaatgcaca
      901 agaattattt gaacatgcaa agaaatcttc tgaaggcaaa gaaatggttg ttcttttaat
      961 gcatgatact tatggaaaac aagaaacagt aaatgcattg gaccaaatta taacatattt
     1021 taaagacaat ggttatcaat ttaaaatttt aatataataa aaaagcaagt ttattgaatg
     1081 tacattttat actcaataga cttgctttta ctttactcta ttttactagc tcgattttta
     1141 caaatttttt ctttcctatt tgaacaacta attcactctc ttttacaata gtgcttaaat
     1201 cctctacttt ttcaccattt acctttactc caccttgaga agctaatctt ctaacttcac
     1261 tcttactctt aacaatctca ttagaaacta gtacttcgat taagtcaaat ccatcttcct
     1321 taacttgaat tgtttgtata tcttcaggta tttgaccttt ttggaataca gatttaaatc
     1381 tttcttcagc ttctttagca gattcttctc catggtataa tgttactatt tctcttgcta
     1441 agttcatctt tatatctctt ggatttactg aaccctcttt caactgtgat tctattttat
     1501 taacttcatc aggatgaaca tctgtcacta aattgtagta tttaattata agttcatcag
     1561 gaatttccat agatttttgg tacattattc ccgcttcttc atctattcca atatagttac
     1621 ctaaactctt actcattttt tctttaccat ctaatccttc tattaaaggc atcattatta
     1681 ctatttgaga ctccatacca aattctcttt gtaatgatct tcccattagt aaattgaatc
     1741 tttggtcagt tccaccaagt tctatatcag cttctaatgc tatagagtca tatgcttgca
     1801 ttaatgggta gaagaactca tgtactgata ttggcatttg tccttcatat ctctttttaa
     1861 aatcttctct ttctaacatt ctcgcaacag taatagttgc tgctaatttt attacatctt
     1921 caaaatttaa cttagctaac cactcgctat taaatcttac aatagttttt tctttatcta
     1981 aaactttgaa aatttgttct tcataagttt ttgcatttgc aagaacttgc tctgttgtta
     2041 aagcttttct agctttagat tttccagttg ggtctccaat ttttcctgta aaatctccaa
     2101 ttattattac tatttgatga cctaaatctt gtaattgttt catctttcta agaactactg
     2161 tatgccctaa atgtatatct ggagcacttg ggtctagacc caattttact atcataggct
     2221 ttccttcttt ttctgattta ataagctttt cccttaactc tttctcatct attaaatcat
     2281 ctacaccttt cataattatt cgcatttgct cgtctatcga tttcattttt cattcctcct
     2341 aaaatattta tataatctat aatttttatt tttataataa aaaaagcttt catcctctta
     2401 aaaggacgaa agcattgctt acgtgttacc accttagttc atcaatatct cacgatatta
     2461 atctcttaga gtacaattac atatactcgt gtactataac gtgtactaat ccgatatctc
     2521 ttaatggttg ttatctttca gagatagtgc tctgagatgt attcaaacta aatcaattta
     2581 ttccatttca ccaaactgga actctctgtt aaataaaatc tagccctact tcttctcttc
     2641 atagcattta tatattcaga ttatttcatc aattacaaga aatttttctt aacttactca
     2701 taatatatta ttatttttat attttgtcaa ccttttttat tattatatac atgttttttt
     2761 acattatact acttttttat attttttacc aatacccaaa atttattttt caatcaaaag
     2821 atttatttgt ttattgacta tattactctg actcaatctt atatttattt tcttatttaa
     2881 aaatgcgttt ataattatta aaccaataac tgttacatca aatataaaaa acagcaataa
     2941 aattattatc aaaaataaaa ttctagcata taataatttt ctaaacttag ctattataaa
     3001 gttttgtagt gacatacatg agtctctaat gataaaatag agacttatat aggttattga
     3061 tataaagatt gtttttaaat aaacagaatc aactttaaca ttcaacagtt ttagaacttc
     3121 aaaaatggct agataagaaa cacatatgta aaaagctttt tttgagcaac ataaaaattg
     3181 gtttaaactt ctaaaattct taaatattaa aaattttttt agagaattag aatctaaaat
     3241 atgcaattta ttccctaaaa ttaaacttaa taaatatatg caaaacacca taccaaaagg
     3301 aaacaatgct atcattaaat aataaataaa atttgtatac ccattaaaat aaatccattt
     3361 gctatatcct tgaaattctt tcataaaact ataacctatt aatgttgaag catatatatc
     3421 aataaaaacc attacaataa gcccaaatac tgaaccatat actaaatcat ccataaccat
     3481 tgtaggctta tttattagat agccacacat tataccaagt aatacactct gacttgccca
     3541 aatagcagtt cctagatttc caagtaccag agaaattatt aaaagcgaag taattccaga
     3601 taaaatggta tactttaaat cacatttcaa gcagattata caaaaaaata ctggcactat
     3661 aaaatctaag taaatagcat atccaaatcc agtactcaca gctataattg ttactactat
     3721 gaataaagat gacaataaag ctgcttcagt taatttttta gtagtattca aaacactgcc
     3781 tccataaatc ataaattttc taactaaagt tttgttttta atatagtata tcacttttta
     3841 aaaatatttt taataaatat aaattttaat
//
LOCUS       DAYATN010000223         3747 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.227, whole genome shotgun sequence.
ACCESSION   DAYATN010000223 DAYATN010000000
VERSION     DAYATN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.227"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            446..2257
                     /locus_tag="ACGI8A_003619"
     CDS             446..2257
                     /locus_tag="ACGI8A_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0181582.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATVLSKTFNIKETCARICSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2345..2926)
                     /locus_tag="ACGI8A_003620"
     CDS             complement(2345..2926)
                     /locus_tag="ACGI8A_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181583.1"
                     /translation="MSYLLKINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDSSNFNY
                     TNYYMEFEAIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
     gene            complement(3362..3568)
                     /locus_tag="ACGI8A_003621"
     CDS             complement(3362..3568)
                     /locus_tag="ACGI8A_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181584.1"
                     /translation="MNQSQEKDLEFIKNHWDLTIIEKERNVTRIIRYSLIILAIIAFF
                     LPTKIKFSIYIFISLFCWLMYLVL"
BASE COUNT         1428 a          374 c          508 g         1437 t
ORIGIN      
        1 agatataatt taagtaatat accaaaaaaa aggaatatat ttacataata tgataaaata
       61 aattcaaaga gttaaattgt aggtaaatag tattttaatt atttattaaa atatagatta
      121 tgtatatgcc aaatttaaaa agagacataa agacattata caaatatatt attggggtga
      181 tattttgtaa gaaaagaagt gatgcagtaa attaattgag taatttaaaa atgatttaat
      241 gataatgttg atgaatgttt aaaaagaaat tttagaaaaa cgtatattgc tgtttgtgtt
      301 ggaggtgatg aatttgtaga atttataggg tataaatgat taaactattt aagataaata
      361 catatcactg taaaatattg ataatcattt tttataagag attgattatt tgcaaattat
      421 atatattaca aatgaggtaa caataatgaa gaaaaaatat gattttttaa tttattctgc
      481 tatttggcta gttattatta ttctttttat attttctcta tatcatagta tagagcatat
      541 taaaattata agtcacactg gtactattcg cagtgaaact caaaaggttg ttaagcaaga
      601 attgaataat gaaagaaatg atgacttgat aaaacgactg gataatattc tgataaagct
      661 aagaacaggt aatggagaaa atggatttca aagatgtgat aataaagaat ttcaacaaaa
      721 actaaatcaa atggattcta tgtgggaatc tatgaagaaa gaaattataa aagttagaaa
      781 tggtgcttca ggtgataaat tatataaatt gagtgaagag tattctgtgt tgtctaatca
      841 aattgttttt atatctgaaa aacactctaa tgcaaaattg tattcttttg ctactgcact
      901 atttatttat cttatattct caacaataag tcttttaatt tgggaatatt ataataaaaa
      961 aaggtttaaa agaatatttt atactgataa tttaacaaaa ataaaaaatc aggttgcatt
     1021 tgaaaacaga gcgattgaaa ttttgcacaa tgcatctaac aaagagtatg tactgctaaa
     1081 tattgatatt gacaatttta aatatattaa tgatacacat ggatatgaat atggagataa
     1141 agttctaata atagttgcaa ctgttctttc taaaactttt aatataaaag aaacttgtgc
     1201 aagaatttgt tcagataatt ttgtaatact tgcaaaatat agagattctc ttttagagga
     1261 tattcgggaa atgctaacta atgctataat ctctgaatta gatatgaatg taactcaaac
     1321 tatatcttat tgtataggag cttacttagt tgaaattgat aatttaggtt ataaatctat
     1381 caattctatg atggataagg caaatattgc acacaaggta agtaaaacaa gaggaatttc
     1441 ctccacagta tggtataatg aaaatctgtt aaaacagtta caaatggaaa atagtattta
     1501 taattatatg tataaagcga tagaaaatga agaatttcat atgtatttgc aacctaaatt
     1561 tcaaatatct tctttaaatg ttgtgagcgc agaagctctt gtaagatggt tttctccaga
     1621 acttggtttt ttatcaccag atgaatttat tccattgttt gaaaaaagtg gatttattat
     1681 tgaattggac ttttatatgt taaaaaaagc gtgttcattt gtaaaaaaaa cgtttatgaa
     1741 aaaaaatcaa tatacatatc cgattgcagt taatttttca agggttacta tttaccaaaa
     1801 tagtttttat caaaggtttt tagatactgt taaagaatat gaaattcctt ttaagtatat
     1861 agagatagag gtaactgaaa gtgcttttaa tgaaatatct caaccagtta tttcaatttt
     1921 agaggaactt aaaaagttag gatttttaat ttctatggat gattttggat caggatactc
     1981 ttcattaagt cttttatgtt ctctatctat taatggattg aagttagata aaagcttact
     2041 taaagaaaca tttaaccggg agaaagtata tagtattatt cagtgtatta ttgaaatgtc
     2101 acatcgtata ggcatgtctg ttgtatgtga aggtattgaa actaaaaaag acttggaatt
     2161 tttaaataca gttaaatgtg atgtaggaca agggttttat ttttctaaac cgatagaaga
     2221 aaaagaattt tttaacaaat atgtaactaa aaaataatat aattattttt atcagtctat
     2281 acattgagtc attttatatc tcgttaaatg actcaatgca ctgttatgta ttttaaaata
     2341 taaattattt aaaacgtttt ttatacttat atagaaattc ttctatattt tttgattgtt
     2401 ctcttatttc aactgcttct atatctagta aataaagtat agaatcattt gtaagttcaa
     2461 catattcacc atcttctgaa ccgagtgcat gtctctcaat ttttaatcct ttttcttcat
     2521 aatatttttt tgtttctata tataatgatt ctcgcttttg tatgtagtct ttcacttcat
     2581 tatttggaaa gttatatgaa tccactaatg tttcgccatt tataaaagtt ttctcggtag
     2641 caactttttc atcccaacaa tggatttctt cccaagtaaa aaatatctca gttccatctg
     2701 gaatgaatga aaacctggta ataatatata aataattatt gaaatttact tcataatatt
     2761 caggaatagc ttcaaattcc atatagtaat ttgtataatt aaagttacta ctatcttttt
     2821 ttacaaatgg tattttctca ataaatttat atattgattg ttcattttta aaaattccta
     2881 tatcatagct tctttcaaat tgattaattt ttaataaata tgacatatat tctccttatt
     2941 tgttgaaata taatttttat aactctacta attttaaaca tttttaaata aatatttgta
     3001 tataccttat ataatattat agtaattagt tttaaatcaa tagaatagta tgtatttatc
     3061 taatgcggca atataattta aagctggtgt tgtagaaaaa ctaagagtga atatagcaaa
     3121 gagaagcaat agtattttaa actttgtctc tcttattttt tgaaaactaa cattaaaaca
     3181 taaggtataa ataatattat catataaatg aaaacaaatt tgaatttttc tctggtcgat
     3241 gtatatagta agtttgttga tatgagaaaa attattattg ataatataca tgcatagaaa
     3301 ggaaatggta tataatattt tcttaagttt cttttttagg catttctatt ggcattttag
     3361 gctatagtac taaatacatt aaccaacaaa ataatgaaat aaatatatat atagaaaact
     3421 taattttcgt gggaagaaaa aaagcaataa tagcaagtat tattagagaa taacgaataa
     3481 tacgagtaac atttctttct ttttctatga tagttaaatc ccagtgattt tttataaatt
     3541 caaggtcttt ttcttgactc tgattcattt aatcatctcc tgttaatagt ataattttaa
     3601 agctattact ttgttttatt ttaacatatt tatacactaa agtgaatttt atgattagat
     3661 ttatttaatt atttctgaat taaaacttat tagaattctc tattttgata atttcaaact
     3721 ctattgattg tatgataatt gaaacta
//
LOCUS       DAYATN010000224         3587 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.228, whole genome shotgun sequence.
ACCESSION   DAYATN010000224 DAYATN010000000
VERSION     DAYATN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.228"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(71..1918)
                     /locus_tag="ACGI8A_003622"
     CDS             complement(71..1918)
                     /locus_tag="ACGI8A_003622"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DpnII family type II restriction endonuclease"
                     /protein_id="HFH0181585.1"
                     /translation="MKPILKWAGGKTQLLDILRKQMPTGCDFSSMTYIEPFVGAGALF
                     LYLLEEDIFNKYVINDINSKLINLYIKVRDDLEELITELTNLKEQYISYEEGSSDREK
                     MYYDIRDRFNILTNEKNIENEDCIKQASYFIFLNKTCFNGLYRENSRGEFNVPFGRYK
                     NPSFFDKNELEKLSMLLNKRDSRGELKVKILNKNYLNLENIIDKNCFVYLDPPYRPVT
                     IGGFNSYNKGNFNDDSQIELSKFYKVLDKIDTKLMLSNSDPKVLDEEDTFFDDLYSDF
                     NIKRVHAKRFINSKGNGRGFITELLITNYEEVGNQMELVKNIHKNKSKDEIFKELLEN
                     LKGTIASWDYYVNWNKALENTRKIEVQLNILNYLIGKEDIENETKNLLKEYPEVLSVI
                     PIVIASRDNEFKILDPSDEDMFKYKLFNFKKRKSISDEESELATEFLKKIKFLDLLKQ
                     KNIKNLVDYVFGVEVGLDTNARKNRTGSSMETLVERYVKNMCEKNNWSYLTQATVKKI
                     SEKWSIEVPTDKSKRKYDFAINNNGKIVLVEVNFYNGGGSKLKSVANEFTKLDKYIKD
                     GGFTFCWITDGLGWLESSIPLRDAFDKMDYIFNLDMVDKGSLEYIIKNL"
     gene            complement(2346..2695)
                     /gene="ssrA"
                     /locus_tag="ACGI8A_003623"
     tmRNA           complement(2346..2695)
                     /gene="ssrA"
                     /locus_tag="ACGI8A_003623"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2853..3308)
                     /gene="smpB"
                     /locus_tag="ACGI8A_003624"
     CDS             complement(2853..3308)
                     /gene="smpB"
                     /locus_tag="ACGI8A_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFH0181586.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         1181 a          644 c          341 g         1421 t
ORIGIN      
        1 attttatata atatataagt ctaaattaac ttgcaatata aaagcgcctc ttaatatagg
       61 cgctataatg ttataaattt ttaattatat attccaatga acctttatca accatatcta
      121 aattaaatat ataatccatc ttatcaaatg catctcttaa tggaatactt gattcaagcc
      181 atcctaaccc atctgtaatc caacaaaatg taaaacctcc atcttttata tacttatcta
      241 acttagtaaa ctcatttgca acagatttta acttagaacc tccaccatta taaaaattaa
      301 cttctactaa aacaattttt ccattattat ttatagcaaa gtcatacttt cttttactct
      361 tatctgttgg aacttctata gaccatttct cacttatttt tttcactgta gcttgtgtta
      421 aataactcca attattcttt tcacacatat tctttacata tctttctaca agagtttcca
      481 tactacttcc agttctattt tttctagcat tcgtatcaag cccaacctct actccaaaca
      541 cataatctac taaatttttt atattttttt gttttaataa atctaaaaac tttatttttt
      601 taagaaattc tgtagctaat tcgctttctt catcacttat actctttctc ttcttaaaat
      661 taaacagttt atatttaaac atatcctcat ctgatggatc tagtattttg aattcattat
      721 ctctactcgc tattactatt ggtatcacac taagaacttc tggatactct tttaataaat
      781 ttttagtttc gttttctata tcttcttttc ctataagata gtttaagata tttaactgaa
      841 cctctatctt tctagtattt tccaaagctt tattccagtt aacataataa tcccagctag
      901 ctatagtacc ttttaaattt tcaagtaatt ccttaaatat ttcatcctta ctcttatttt
      961 tatgaatatt cttaaccaat tccatttgat tccccacctc ttcataatta gtaattagca
     1021 attcagttat aaaacctctt ccattcccct tagaatttat gaatctttta gcatgtactc
     1081 tttttatatt aaaatctgaa tacaaatcat caaagaaagt atcttcttca tctaaaactt
     1141 ttggatcaga attacttaac atcaattttg tatctatttt atctagaact ttataaaatt
     1201 tacttaactc aatttgacta tcatcattaa aattaccttt gttataggaa ttaaatcctc
     1261 ctatagttac tggtctatat ggtggatcta gatacacaaa acaattttta tctattatat
     1321 tttctagatt taaataattc ttatttaata tttttacttt tagctctcct cgagaatctc
     1381 ttttatttaa aagcatggat aatttttcaa gttcattttt atcaaaaaaa ctaggatttt
     1441 tatatcttcc aaaaggtaca ttaaattcac ctcgagaatt ttctctatat aatccattaa
     1501 aacatgtttt atttaaaaat ataaaatatg aagcttgctt tatacaatct tcattttcta
     1561 tatttttttc atttgtaagt atattaaatc tatctctaat atcatagtac atcttttctc
     1621 tatctgaaga accttcttcg taagatatgt attgttcttt taaattagtt agttctgtta
     1681 ttaactcttc caaatcatct ctgactttaa tatataaatt aattaactta ctatttatat
     1741 cattaattac atacttgttg aaaatgtcct cttctaacaa atatagaaat agtgcacctg
     1801 cacctacaaa aggttctata taagtcatgg aactaaaatc acaacctgta ggcatttgtt
     1861 ttcttagaat atcaagtagc tgtgttttac ctccagccca ttttaaaatt ggtttcatgc
     1921 aacacctctc tcagttatat tgttatataa ttatataaaa taagattcta aaatacaagt
     1981 actcatcaga acactatatt tatatcaaat ctttccaaaa tctaagtatt attttatcct
     2041 aacacttttc aaaacctcta aataatatac aatattctct aatatttact tactaaaaat
     2101 ttaaatctat ctttaagtat aacctaaaac agattataag ttacgtagtt tttaatataa
     2161 aatatacttc tacaagtaaa gctttaaata tcaattactt atttcataat taatgactat
     2221 gtaccctcaa aaaaacctca ttttctatgg ttggggtacg gttggtggat ttcatcacca
     2281 aaataaaaaa tactagtcaa taaagactag tatttccaag tatttcatca ttcttttttc
     2341 aaaattggtg gagacgaggg gagtcgaacc cctgtccgca aatcttttcc ttgctgcatc
     2401 tacatgctta gccaacttca ctaatatctc acctcacata gcgccaagtg gcaggctcta
     2461 tgcttagcca gtcccaatta atctacttca ggtcgagaca cccctaaagt agttccctgc
     2521 ataatgacgt ccagtcttag tcggcaggag gactaagagg gacgagcagc aactaggctg
     2581 ctatcgcgaa attatcgtct gcgtttattt taaagttccc cagtttttac gtgacccaga
     2641 gtaacacgac atgcagcctc aagttccaaa cccacgtcga aacctttacg cccccttgag
     2701 tagtttatag tccagtagac ttttcgcaaa ctttatcacc atttttatta aatttttaat
     2761 gtatatataa tataatactt ttaagaataa aaatcaatgt tatgttctag ataataaaga
     2821 attttattct aaaaaataca gttttatcta gtttaatact tacctgtcat ttctctttct
     2881 atacgtcttt gtgcatcttt cttagccata tcttgacgtt tatcatatag ttttttacct
     2941 tttgctaagg caatctctac tttaacttta ccgtttttta gatacaaact aagtggtata
     3001 agtgaatacc cctttatagt agttgctcca ataagctttc ttatttcatg cttatgaagt
     3061 aatagttttc taactcttaa agaatcaaca ttgaatatat ttccttgctc atatggactt
     3121 atatgaactt gttttaaaaa tacttcgcta ttatctacag atgcaaatcc atcacttagg
     3181 ttaactttgc cttgtcttat tgattttact tctgttcctt ttaattcaat tccacattca
     3241 taagtttctt ctataaaata ttcatgtctt gctcttttgt ttgttgctaa tattttttta
     3301 ccagccatat aaatctcacc ttctttgcta gaaatttatt ccataaatat tagaaattta
     3361 ctaaatattt aatgttgcca aattatttga ctaacttgta tgataacata tttaggcatt
     3421 ttagtcaata tttcactgat atattaagtt agttaggcta tatataatta agtaatataa
     3481 atgtatgaaa atagctaaaa aactacttcg aatatactta atactaaact aactctaacc
     3541 agtaatacta atttataaat ttcaaaacaa aaatattaca tattata
//
LOCUS       DAYATN010000225         3481 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.229, whole genome shotgun sequence.
ACCESSION   DAYATN010000225 DAYATN010000000
VERSION     DAYATN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.229"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(107..1674)
                     /locus_tag="ACGI8A_003625"
                     /pseudo
     CDS             complement(107..1674)
                     /locus_tag="ACGI8A_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(1667..2192)
                     /locus_tag="ACGI8A_003626"
                     /pseudo
     CDS             complement(1667..2192)
                     /locus_tag="ACGI8A_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ia"
     gene            complement(2581..3327)
                     /gene="cdtR"
                     /locus_tag="ACGI8A_003627"
     CDS             complement(2581..3327)
                     /gene="cdtR"
                     /locus_tag="ACGI8A_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HFH0181587.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         1157 a          453 c          380 g         1491 t
ORIGIN      
        1 gaagaagata atatttaaat aaaaaatacc ttataaagtt aattttttaa tactttataa
       61 ggtaaataca tattgtattg tggggataaa tttaaatcat ttgatactaa tcaacactaa
      121 gaaccaataa ctctctatca tcttgagtaa ttgtatatat tcttaatttc ttgtatgtca
      181 taatattttc tccacctgta aaataactcc taagattaac acaatcagtt ttaggttgat
      241 ttgtatctcc taaataatta cgcaattctt tatctttagt ttcaatatct ttaaatccaa
      301 aatattgtaa agtagcttca actctatatt tttggacata atcgagagct tctttattag
      361 tttatgttgg tgtaaaatac ataccattta tataagcctt tattgtactt ggattaaatt
      421 taaaatctgc agaatatatt ttatgtgtaa ctattatttc ttgaacagtt ggaattttaa
      481 cttctggttc atcaagtatt tcaggagtac tatttagctc tgtaatagat aagttatcta
      541 agtatattgt atcactatca attaatgcta tatttatatt agaagaatct ttttcagtag
      601 tttcaaattc ataactaaat tttgtatatc cttgttctag taccaaataa tccgtttttt
      661 cttcttttac ttttatattt acaattattg aattatatgt taaaggattt tttgaatttt
      721 actaaaaaca taacgtttat aaggttttag ttcagacata ggtattttaa tcttcgtctc
      781 accatttaat ctatttgctg agccttttaa accatcttga tttgtagtat taacattact
      841 ccatatacta gggtaattat tgtaatcatc aaaattagta aagtaagttg gtgtttttat
      901 aagtatattc attcctcttt caagtttaac attacatatt tgtttatcag acaaagtttt
      961 taaactgtct ttaatcttat tgcctatatt atcatcaaat atgagttcaa cacaactttc
     1021 atctattggt atattattaa aatataacaa accatctttt ttagtagtgc caaaagtttt
     1081 ttcaattgct tctccaattg taagttcagg tgttttatct tctgggacct gtaaactttt
     1141 agcagtaatt cttcttttgt aagattcatt ttctgtatct aatataatag atacagaaat
     1201 actgtcaatt tgacctatat cgtcttacca actatttcat tctgttacta tttgtccaga
     1261 accatttttt gtaccaaaat ttccacttac ttgtgttgtt tctaatttaa tttgctttcc
     1321 atcatctaat ttttttaatt aattataatt tataggaatc agtctagaac taaattgata
     1381 cattgtgtta agagccatag gtgaaaatcc ttttttgaga taagtatttc caggagatag
     1441 attattgcca atttgatttt cttgtgcttt gatagttgat aatgcatctc catctaacac
     1501 taaatttgtt gttggtgtca ctttgtacat aggtgcagta cctgtgttgt agtatctaat
     1561 atttgcattt atatatgcag attctccttt gtttatactt aatccagtat ttcatgatta
     1621 tccattgcta ttttgaacag cagttgaatt atctgttgta tggtaataat ttgtcttgta
     1681 tagtttcttt aaactcatta aatttttcta atgaaatttc gttttgattt tttgttctta
     1741 ttactttatt aaatgcaaat ttttctggag attcaaaata atatacatac ataggtttat
     1801 ctattttatt ttttgatata gcatttcgaa gttctctata ttctttttct caagaatttg
     1861 cttctatttg ataatcataa aaataatttc ttgtctgaga atatttgctt atctctatag
     1921 aatctttttt atatgatcct aatacttctt tttcagattt ttcaagtttt tgatcttttc
     1981 tttctcattc tttagccttt tctttatctt taaaaaaatc cttctgatct ttctatagga
     2041 gccttgtaag tagtattaca aacttttcca ctatagcttt gtaaatcttg tgcataaatg
     2101 ttattaggta acaaactacc taaagttaga tataatatca acaatataga tatacaatta
     2161 ctaatacttt tatgtttcct aaattttttc atttattctc cctacaaata ttagtttagt
     2221 taacttttga attttaattt atttagatta tataatctaa ataagaattt tttatataaa
     2281 tttcataaaa tatgtaggta tatatcataa aaatatgtat ttatatgata tgtcagtaca
     2341 aaataacaac tgaatatttt aaagagacta tttcgtactt ttatgattta atgagctatc
     2401 ttatcgaatc ataatctagg aacaattgtt ataatatcta atagtagtta tatatgatat
     2461 agtcagtatg tgttttttca caatatgatt attatataga ggattcttag ggattactct
     2521 ttttgtataa aaaagagatg gttcaaattt ttatcttgag acatctcctt tttctattta
     2581 ttatgtttta ataatattct ttaaaatatt tctatatgta ttagatatac gacatatatg
     2641 gccattactc attttaatat tatttttctt taggtcgaga gaaacaatat gatatttatt
     2701 aactacaaaa gatttatgta cacggataaa gcatgcatct aaatctggta aagactttaa
     2761 acttttatag aattcaaata ttctagaagt agtcactaat tttattttat gtggaatagt
     2821 gcttgtttca aaatatataa cttcatcaaa tgaaattttc catagtgaga aatctgaata
     2881 gatagtaaaa aaattagagc gataattttt ttctttaatg tatcttttgt gagcaatttt
     2941 aatacaagct tttattttac tttcagcaat ttccatatca tgttttagta taaagtccat
     3001 aacttctaac ctataatcga agatccctaa aatcatttct atatgatttg taagaaatat
     3061 gattggacta atataatctg attctctaat tttttgtgct atccataatc catcacataa
     3121 ttcattactt atcttatatt ttgcgtctac aactataaaa tatattctta ctttattttt
     3181 ttcagtaagc tcttttaata acaaatttgc attatgatat aatcttacaa catcattatc
     3241 aaaaccttct tttattaaaa tattatttat tttttctttt agtttcattc caaaacaaac
     3301 atcattatca aagattaata tatccactaa ataccctcct ataaaaaatt caaaagtaaa
     3361 aaaacaaaag tattttatag aaaatacata aaataacact aggagataaa taagattaag
     3421 gatagttaga attaaggtat atttatgctt gtttatatag tatatcttat acttatagtt
     3481 t
//
LOCUS       DAYATN010000226         3471 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.230, whole genome shotgun sequence.
ACCESSION   DAYATN010000226 DAYATN010000000
VERSION     DAYATN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.230"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..287)
                     /locus_tag="ACGI8A_003628"
     CDS             complement(<1..287)
                     /locus_tag="ACGI8A_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFH0181588.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTITKEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFN"
     gene            complement(332..562)
                     /locus_tag="ACGI8A_003629"
     CDS             complement(332..562)
                     /locus_tag="ACGI8A_003629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181589.1"
                     /translation="MKEYMIWFKSGNSISGIVDEDVADKLMQDFMEADSDCKDLKVYL
                     DEDGTTVIDLSQIEAISINNCNENNNIGFSKS"
     gene            complement(605..802)
                     /locus_tag="ACGI8A_003630"
     CDS             complement(605..802)
                     /locus_tag="ACGI8A_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181590.1"
                     /translation="MFYLLLQVISICLSITVFMYVTGNIVFKIYLKSLRKEYESNIHE
                     VSDEILKRIRKELNKSLDESL"
     gene            complement(803..1195)
                     /locus_tag="ACGI8A_003631"
     CDS             complement(803..1195)
                     /locus_tag="ACGI8A_003631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181591.1"
                     /translation="MAKFKKKSEEVEAFKWILGSPNTPIWFYQAFEKGTIWLDESLNS
                     VNRKGEVKKTICIKYKNGVIRATNGDWIIKDSEGKIYSCTCDEFEKYYKDLDNETKIS
                     NGGLECLREIALKDVNFKFELTGIKEEK"
     gene            complement(1527..1688)
                     /locus_tag="ACGI8A_003632"
     CDS             complement(1527..1688)
                     /locus_tag="ACGI8A_003632"
                     /inference="COORDINATES: protein motif:HMM:NF025569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFH0181592.1"
                     /translation="MNCPVCGKDVDVFDICDNCNWQNSGPKETNYKGPNKMSLKEAKE
                     AYEKGKKVI"
     gene            complement(1691..3052)
                     /locus_tag="ACGI8A_003633"
     CDS             complement(1691..3052)
                     /locus_tag="ACGI8A_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFH0181593.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSIKYYLELEKQYKLAMSNIEKDI
                     LIWYNRFTENEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKVLV
                     NEFSKKFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLKTSNICRE
                     LDGKVFDMKDYQVGITAPPFHCHCRTTTAPWFEDEEGYRAARDENGKTYYVPSGMKYK
                     EWYEKTFITDKKDDILSNKSWLKADFPTEKKFKKHVEKHLNEYGNITAEEYLSTARNL
                     LSEQLSNDVEGFLSKDNFLFKYRKSTNDFAVGRADGKISTLFKPKEGYNYWLEQIKEY
                     KEV"
     gene            complement(3042..>3471)
                     /locus_tag="ACGI8A_003634"
     CDS             complement(3042..>3471)
                     /locus_tag="ACGI8A_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0181594.1"
                     /translation="IIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKA
                     IRELLWFVCEYLKISGNKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVS
                     NHPWVEDVNDELERLKKQEDTQKEYDDLIPNNQDGVIDET"
BASE COUNT         1064 a          647 c          318 g         1442 t
ORIGIN      
        1 taattaaagt ccttttcttc aatttctttt ttgtaggttt cttccaaagt tttcttatca
       61 gtttcataag ttgtttttag attctctaca tcttcttttg tcatacctcc ttcaaacttt
      121 ttaatagttt cattagctgt attaagttgt gtttcaagat ttgtataatc ttctttagta
      181 attgtagtct cttttatttt cttttctata gatttttgaa gagaagctac atcaattttg
      241 ttatcctcta cttttattcc ttctagcaat tcttttaacc aatccatttt aaatatctcc
      301 tttcattttt tacaaaataa aaaagcccta tctaggactt actaaaacca atattattat
      361 tctcattaca attatttatt gatatagctt ctatttgtga taaatctatt actgttgttc
      421 catcttcatc taaatatact ttcaagtcct tacaatctga gtcagcttcc ataaaatctt
      481 gcattaactt atcagcaaca tcttcgtcta ctattccaga tatgctattg ccgcttttaa
      541 accaaatcat atattctttc aataacagaa acctcctttt gttttctacc taataaaaaa
      601 cttcttaaag actttcatct aatgatttat ttaattcttt tcttattctc tttaaaattt
      661 catcagaaac ttcgtgtata ttagactcat attcttttct taaactttta agatagattt
      721 taaagactat attacctgta acatacataa atactgtaat acttaaacat atacttatta
      781 cttgtaacaa cagataaaac atttattttt cctccttaat tcctgtgagt tcaaatttaa
      841 aattcacatc ttttaatgct atctctctta aacattccaa tcctccatta gaaatctttg
      901 tttcattatc caaatcctta tagtactttt caaattcatc acatgtacaa gaataaattt
      961 taccttcact atcttttata atccaatctc cattagttgc cctaataact ccatttttat
     1021 attttataca gatagttttc tttacttcac ctttacgatt cacagagttt aaagattcat
     1081 caagccatat agttcccttc tcaaaagctt gataaaacca tataggagta ttaggacttc
     1141 ctaatatcca tttaaaagct tctacttcct ctgatttctt tttaaattta gccatattat
     1201 ttatccccct ttaaattttt aatcatatct tcattgctaa ctagtaaaaa aaatagtatc
     1261 agtatcacac ctaaaataaa attaagcagt ggaaataacg ctgggaaaat aaaattatat
     1321 ttttttcttc ttctgatatt cttactttta agtaattcaa ttaattcctc attactatca
     1381 accttcatgt tgaataaata aagccctgta caaaacacaa ttattgataa aataagtata
     1441 gatataattt aagcatttca aatcaccctc gcaataaatt tttacataat aaaagcacct
     1501 actaatttat aacttagcaa gtgcttttat attacttttt tacctttttc ataagcctct
     1561 ttagcttctt ttaatgacat tttatttggt cctttgtaat tagtttcttt tggtccactg
     1621 ttttgccaat tacaattatc acaaatatca aatacatcta catctttacc acatactgga
     1681 caattcatgt ctaaacctcc ttatattctt ttatttgttc tagccagtaa ttataacctt
     1741 cttttggctt aaataaggtt gatattttac catcagctct tccaacagca aaatcattag
     1801 tactctttct atatttaaat aaaaagttat ctttacttaa aaatccttct acatcattac
     1861 ttaattgctc tgataacaaa tttcttgcag tagataaata ttcttcagca gttatatttc
     1921 catattcatt taagtgtttt tctacatgct ttttaaattt cttttcagtt gggaaatctg
     1981 cttttaacca actcttatta cttagtatat catctttttt atcagttata aatgtctttt
     2041 cataccattc tttatatttc ataccagatg gcacataata tgttttccca ttttcatctc
     2101 ttgctgctct ataaccttct tcatcctcga accaaggagc tgttgttgtc ctgcaatgac
     2161 aatgaaatgg tggagctgtt attccaactt gataatcttt catatcaaat acttttccat
     2221 ctaactctct gcatatattt gatgttttta aatctagtgt ggcaataatc tcatactttt
     2281 ctacatctaa atcattaaaa caatcttttc ttgaagctga tgcaaagaaa gctgattcag
     2341 tcattattaa attcttagct tgtgatttag atacattaaa cttcttagaa aattcattta
     2401 ccaaaacttt tggattttca cctctaataa ttgattgagt tagcttagta tgtaactcat
     2461 tgattaaagc aggtctatgc ttaccccaaa ttctttcact aaagtttaat ccatcactag
     2521 tccaaggctt agaaataact ttatttattc tgttagtatc aagactcatt aaactccaac
     2581 caacgtttac tccttgttga acattaaaag ctgtatgata gtatccactt gtataaatat
     2641 ctctcattag tttatcaata ccatcaagtt catttccata taaaacttct acttgttgct
     2701 gtatttgcag ttttaaagct tcaagccttg ttatatgaac tcttgcacta gcattttcta
     2761 actctttcat ccacttttga tttatagcat tttctttacc atatttaata tattcttcta
     2821 cactccattt aaactcttct agttctcttg tatttagcag tttcttagct tccaataaag
     2881 atattccctc attttcagtg aatctgttat accatataag tatatctttt tctatattag
     2941 acattgctag tttatattgt ttttctaatt caagataata ttttatactt ttattattct
     3001 gagcttcttc taattgttca aatctcttcc tccaataatc tttatgtttc atctataaca
     3061 ccatcttgat tattaggaat taaatcatca tactcttttt gagtatcttc ctgtttttta
     3121 agtctctcaa gttcgtcatt aacatcctcg acccaaggat ggttagaaac aatagtttca
     3181 tctgatacaa ttccagttga tttagctgcc atatctatct tttcagcttc atttattatc
     3241 atagagtgat taaaagtaat ttgaactgtt ttataatcat agctcttatt accacttatc
     3301 tttaaatact cacacacaaa ccataaaagc tctctaattg cttttttaaa cttcttttca
     3361 gtcttagaac attttaagtc aagtagtgaa tataaaaatt taagtgctac acccgatttg
     3421 tcacctgtgt tttgagattc tggattaact ccttgaccaa agataattat a
//
LOCUS       DAYATN010000227         3321 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.231, whole genome shotgun sequence.
ACCESSION   DAYATN010000227 DAYATN010000000
VERSION     DAYATN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.231"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1190
                     /locus_tag="ACGI8A_003635"
     CDS             <1..1190
                     /locus_tag="ACGI8A_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0181595.1"
                     /translation="LVDQKVGYSVGNPIVFNADDDNLTKLLNDLLGEEFDDTITELYL
                     NASNKGIEWLHPYINRKGEFKYVIIPAEEAIPIWDSKRQRELVAFIRFYYIEDIDGNK
                     IKRVEYYTENDVTYFIERGNSFIQEFLYDEYGKMTDIQEGHFRINNKEQGWGKVPFIP
                     FKNNEKCVSDLNFYKSLIDIYDNNISTLADNLDEIQEVIYVLKEYPGTSLQEFIDNIR
                     YYKSIKVDGGGGVDKLEINIPVEAKKELLDRLEKNIIIFGQGVNPESQNTGDKSGVAL
                     KFLYSLLDLKCSKTEKKFKKAIRELLWFVCEYLKISGSKSYDYKSVQITFNHSMIINE
                     SEKIDMAAKSIGIISDETIVSNHPWVEDVNDELERLKKQEDTQKEYDDLIPNNQDGVI
                     DET"
     gene            1180..2715
                     /locus_tag="ACGI8A_003636"
     CDS             1180..2715
                     /locus_tag="ACGI8A_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFH0181596.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINNIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRHALVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKVFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSNMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     KGE"
     gene            2717..2965
                     /locus_tag="ACGI8A_003637"
     CDS             2717..2965
                     /locus_tag="ACGI8A_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181597.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            3035..>3321
                     /locus_tag="ACGI8A_003638"
     CDS             3035..>3321
                     /locus_tag="ACGI8A_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFH0181598.1"
                     /translation="MDWLKELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANEAIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFN"
BASE COUNT         1430 a          335 c          597 g          959 t
ORIGIN      
        1 ttttagttga ccaaaaggta ggctatagtg tcggaaatcc catagtattt aatgcagatg
       61 atgataatct cactaagctt ttaaatgact tactaggaga agaatttgac gatacaataa
      121 cagaactcta cctcaatgct agtaacaaag gtattgaatg gttacatcca tatattaata
      181 gaaaaggtga gtttaaatat gtaataatac ctgctgaaga agcaattcct atttgggata
      241 gtaaaagaca gagggaatta gttgcattta ttaggtttta ttatattgaa gatatagatg
      301 gaaataaaat aaaaagagtt gagtactaca cagaaaatga cgtaacttac tttattgaaa
      361 gaggtaatag ttttattcaa gaatttttat atgatgaata tggaaaaatg actgatatac
      421 aagaaggtca ttttagaata aataacaaag aacagggatg gggtaaagtt ccatttatac
      481 cttttaaaaa taatgaaaag tgcgtatcag atttaaattt ctataaatca ttaatagata
      541 tatatgacaa taatatttct acactagcag ataacttaga tgaaatacaa gaggttattt
      601 atgtattaaa agaatatcca ggaacaagtc tacaagagtt tatagataat ataagatact
      661 ataaatcaat taaagtagat ggtggaggtg gagttgataa actagagata aatataccag
      721 ttgaagctaa aaaggagctt cttgatagat tggaaaagaa tataattatc tttggtcaag
      781 gagttaatcc agaatctcaa aacacaggtg acaaatctgg tgtagcactt aaatttttat
      841 attcactact tgatttaaaa tgctccaaaa ctgaaaagaa gtttaaaaaa gcaattagag
      901 aacttttgtg gtttgtatgt gagtatttaa agataagtgg tagtaaaagt tatgattata
      961 aatcagttca aattactttt aaccactcaa tgataataaa tgagtctgaa aaaatagata
     1021 tggcagctaa atcaattgga atcatatcag atgaaactat tgtttccaat catccttggg
     1081 ttgaggatgt taatgacgaa cttgagagac ttaaaaaaca ggaagatact caaaaagagt
     1141 atgatgattt aattcctaat aatcaagatg gtgttataga tgaaacataa agattattgg
     1201 agaaagagat ttgaacaatt agaagaagct caaaataaca aaagtgtaaa atattatctt
     1261 gaattagaaa agcaatataa actagctata aataatatag aaaaagatat attagcatgg
     1321 tacaacagat ttgccaaaaa tgaaggaata tctttattag aagctaagaa actactaaat
     1381 acaagagaac tagaagagtt taaatggagt gtagaagaat atattaaata tggtaaagaa
     1441 aatgctataa atcaaaagtg gatgaaagag ttagaaaatg ctagtgcaag agttcatata
     1501 acaaggcttg aagctttaaa gttacaaata cagcaacaag tagaagtttt atatggaaat
     1561 gaacttgatg gtattgataa actaatgaga gatatttata caagtggata ctatcataca
     1621 gcttttaatg ttcaacaagg agtaaacgtt ggttggagtt taatgagtct tgatactaat
     1681 agaataaata aaattatctc taaaccatgg gcaacagatg gattaaactt tagtgaaaga
     1741 atttggggta agcatagaca tgctttagta aatgaactac atactaagct aactcaatca
     1801 attattagag gtgaaaatcc aaagaatcta gtaaatgact ttgctaaaag atttaatgta
     1861 tctaaatctc aagctaagaa cttaataatg actgaatcag ctttctttgc atcagcaagt
     1921 agaaaagatt gttttaatga tttagatgta gagaaatatg agataattgc tacactagat
     1981 ttaagaactt caaatatatg cagagaatta gatggtaaag tatttgatat gaaagattat
     2041 caagttggaa taacagctcc accatttcat tgtcgttgta ggacaacaac agctccttgg
     2101 tttgaagatg aagaaggtta cagagcagca aaaggagaag atggaaaaac atattatgta
     2161 ccatctaata tgaagtataa tgagtggtat gagaaacata tagagggtag acttggaaaa
     2221 gaaaaagctg atacccttag aaaaatgcaa ctaaatgaga gtaaagatag aaaacaattt
     2281 gaagaatata aaaaggtatt aggaaatgaa ataccttcta aatttgatga atatcaacat
     2341 atgaagtata atgatactat aaggtatgag gaagctaaaa agctttataa ggatgtaaat
     2401 tggcaagtta agaatcagag aaatttaact agtggaagtg ttcattcagt gccatttgaa
     2461 tcaaaaccaa atagtgtatt tgataattac aaagatggta aattaattca aagaagatac
     2521 tatggtaata caggaaaacc tagattagat ttagacttaa ctgaccatag aaacccaaag
     2581 caacataaaa ttgtacctca taaacatgat tggttagctg atgaaaataa ccatgataag
     2641 gtaaaaagag agaaggatat ggagcttaca aaagctcata aaatagctaa caaagatatt
     2701 ttgaaaggag agtaaaatgg ataataaatt taagaattta caacatttaa ttgattctat
     2761 tgatttagga ttagatatag aatttgactt atataataaa ccatataata tatctattgg
     2821 agatgatgat acaaggttta ttactctttg ccctaatgga gatacaaagt attataaaaa
     2881 tggtaaggat atggttgata attacaacat tgatggtaaa ttactaaagg atttatggaa
     2941 agatatacaa atagtaaata tgtaaaagca cttgctaaat gataaattag taggtgcttt
     3001 tattatgtaa aaaatgaaag gagatattta aaagatggat tggttaaaag aattgctaga
     3061 aggaataaaa atagaaaata acaaaattga tgtagtttct cttcaaaaat ctatagaaaa
     3121 gaaaataaaa gagactacaa ttactcaaga agattataca aatcttgaaa cacaacttaa
     3181 tacagctaat gaagctatta aaaagtttga aggaggtatg acaaaagaag atgtagagaa
     3241 tctaaaaaca acttatgaaa ctgataagaa aactttggaa gaaacctaca aaaaagaaat
     3301 tgaagaaaag gactttaatt a
//
LOCUS       DAYATN010000228         3300 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.232, whole genome shotgun sequence.
ACCESSION   DAYATN010000228 DAYATN010000000
VERSION     DAYATN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.232"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            147..1031
                     /locus_tag="ACGI8A_003639"
     CDS             147..1031
                     /locus_tag="ACGI8A_003639"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0181599.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            1233..2783
                     /locus_tag="ACGI8A_003640"
     CDS             1233..2783
                     /locus_tag="ACGI8A_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFH0181600.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            2812..>3300
                     /locus_tag="ACGI8A_003641"
     CDS             2812..>3300
                     /locus_tag="ACGI8A_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFH0181601.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWI"
BASE COUNT         1355 a          342 c          593 g         1010 t
ORIGIN      
        1 catgttttga tgcttggaaa actagctcaa taacacctta gttttcataa aaataaataa
       61 aataaatagt gtatatgata aaataaatac atattcatat aaataaaaat aaacaataac
      121 tgtctagcat aagaaaggag caaaaaatga aaaaatatag attgttagtt acttgtataa
      181 ttgccatggg aatcataatt ataggaacat caaatccagt tagcaatttt tacaagacta
      241 caaatttgat tagttcagga aatatcacaa atgaaaaagc agataaagat gaaaattcaa
      301 acaaaaataa taaagataaa aatgaagaca aatctaattc taaaagtaat aaagataagt
      361 catcagagga caaatcgtca gataataaaa atgaaattca aaaaaataaa aaacttttaa
      421 tttgtataga cccagggcat caaggaaaag gagatagcaa tttagaacca gtagctccag
      481 gttcttcctc taaaaaagcg agagtatctt caggaacaga gggtattgct acaaaaaagc
      541 cagaatatgt tttaaactta gaagcatctc ttgttttaaa gagtatatta gaatctaaag
      601 gttataatgt aataatgact agagagacac atgatgtaaa tataagtaat tcagaaagag
      661 caatacttgc taatgacaaa aaagcagata tggttgtaag aatacatgca gatagtttaa
      721 ataattcttc aaaaactgga gcatctatat tgattcctga aaaagatgga aaatatacag
      781 ctcctatata tgaagaaagt aataaatgtg ctgaatttat caaacagaat atggaacaat
      841 caggtataca aatcaatgga atcgttcaaa gaggtgattt gactggattc aactggtcaa
      901 aagtaccagc tgtattagta gaaatgggat ttatgagtaa ctataatgaa gaccaaatga
      961 tgtcaaatcc agactaccaa agaaaaatga tgcagtgtat agctgatgga ttggatgcgt
     1021 attttaaata agatatgaat agagaaaaag ttaggttggt attatttgcc taatttttat
     1081 ctctattttt tattgtacat aacacaaaga agaagtttat tttaggttaa attgtcgtaa
     1141 atataactaa aaatattggg aatacttgat ttaagtttaa aaagaatata ttatagtata
     1201 gaatgtgtaa aatttttttg agaggtgttg aaatgctagt agtagaaaat gtgagtcatg
     1261 gatttggtgc gagaacaata ttagaaaatg tatcctttag acttagaaaa ggtgaacata
     1321 ttgcactagt tggtgctaat ggagaaggaa aatcgagttt tttaaatata ataacaaaga
     1381 agcttatgcc agatgctgga aatataaaat ggtcttcaag agctacagta ggttacttag
     1441 accaacatac ggtattaagc aagggaaaaa ctataagaga agtgcttaga gaggctttta
     1501 agcatatgtt tgacttagag caagaaatga ttgctatgta tgacaaaatg ggagaagcta
     1561 gtgatgatga aatgagtaag cttcttgaag aaacagcaga gatacaaact atacttgaaa
     1621 atagtggttt ttatatgata gatgcaaaga tacaagaagt tgcaaatggt cttggtcttg
     1681 gagaaattgg tcttgataaa gatgtaactg acttaagtgg agggcaaaga acaaaagtac
     1741 ttcttactaa acttctactt gaaaatccaa ctattcttat attggatgag cctacaaact
     1801 atttagacga agagcatata acatggctta ctaagtacct tcaagagtat gagaatagtt
     1861 ttgtcttagt ttctcatgat attgaattta taaataatac atgtaatgta atatatcata
     1921 tggaaaatgg agagttaaat agatataaag gaaattatga tgaatttgta agacttaatg
     1981 atataaagaa acgtcaagaa gaacaagctt atgataaaca agttgaagaa agaaaaagac
     2041 tagaggattt tgtagctaga aataaagcta gagtagcaac tagaggaatg gctaacagta
     2101 ggcaaaagca acttgataag atggaaatat tagaaagacc aaaagaaaaa ataaaaccta
     2161 cttttgcttt taaagatgct agagcagcta gtaaaataat ttttgaaaca gaaaatttag
     2221 ttttagggta tgatgaagca ttgacaaaac cacttaattt ccatttagaa agaggaaaaa
     2281 agattgcctt aaaaggaatg aatggtattg gaaaatctac cctattaaaa actttacttg
     2341 gaataataaa accatttgaa ggaaatgtta aattaggtga ctatttggaa gttggatatt
     2401 ttgaacaaga gagttcaaga gaaaatagta atacacctat ggatgaagtt tggagtgaat
     2461 ttccagggct tacaaacttt gaagttagac aggctcttgc aaaatgtgga cttacaaatg
     2521 aacatataac gagtcaaatg agagtattaa gtggtggaga agctgctaag gttagacttt
     2581 gcaaagtaat gttaaaaaat ataaatttct tagtattaga tgagcctaca aatcatttag
     2641 atgttgaggc aaaagatgaa ttgaagaagg ctatcaaaga gtttaaggga acagttcttt
     2701 tagtatgtca tgagccagaa ttttattcag aaattgttga tgatgtatgg aatatagaag
     2761 attttacaac taaaatcgtg tagtgattta acttattgct ggggggattt catgaaaaag
     2821 tttctaaatg tacttttttc aatttgtatt agtattgtta taatagtagg tgttattaat
     2881 tttactgttg gatttaaaca actttattat tttgacattg attacttaaa tatatcagag
     2941 ttatctggtt tatctaaaga tgatataaaa ttgaactacg attatctaat tgattataac
     3001 ttaaataaga atgtgtctga gttcaaatta ccaacattaa aatcttctcc acaagggaag
     3061 atacattttg aagaagtcag gaatatattt caaaatataa ataaattagc taaattatta
     3121 ttggtagttt ctttagtagg aattatattg agtgtaaaaa ataaaaatat aaaaatctta
     3181 aaaactacat caataacact tataattatg ccattattat taacagttcc aattttatta
     3241 aattttgaaa agtcatttat tatttttcat aaattattat ttagaaatga ttattggata
//
LOCUS       DAYATN010000229         3285 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.233, whole genome shotgun sequence.
ACCESSION   DAYATN010000229 DAYATN010000000
VERSION     DAYATN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.233"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(302..523)
                     /locus_tag="ACGI8A_003642"
     CDS             complement(302..523)
                     /locus_tag="ACGI8A_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HFH0181602.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            1103..1360
                     /locus_tag="ACGI8A_003643"
     CDS             1103..1360
                     /locus_tag="ACGI8A_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFH0181603.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            1829..3067
                     /locus_tag="ACGI8A_003644"
     CDS             1829..3067
                     /locus_tag="ACGI8A_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HFH0181604.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYITYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1358 a          296 c          492 g         1139 t
ORIGIN      
        1 agtgtttcaa ttgtcaatat tagtatttta attgttgact ctagttattg ctgatataga
       61 aaatatttaa tttatttcaa ttaattagat taaagtaggg tagcatctaa aatagctaac
      121 atgtttaagt tgaaatacgt ttaatatatt aatttttcaa agcgttaata tattaaagtt
      181 ataaccaaaa ttgaagttat aatctgccaa atctatagcg tattaagtgc attatataac
      241 ttaatctttt atataaagag ttaatttttt aatttgtaaa gcagagtacg tttaaaagac
      301 actatttaga tagtgatgta ctatctaatt ttaaaatatc tacaataggg ttctcttgaa
      361 gacttgctat tgcttctgca acagcataaa catcttcatt taatgcctct gcttttacat
      421 ttgcaaatga tttactttta acaactgcct tacccttatc atcaaaacca gcatccaatt
      481 ttagttttat actagataaa ttttttgttg aaacaacttc cataaaatta cctcctaaaa
      541 atttaatttc aaagatttgt cgactttctt tgttagctat aatactatca tctaagataa
      601 tatcggagtt aaacagtaat ttctaaagat tttttgtgta aatttttata ggaaaaattt
      661 tacttttttg ctgatgaaaa agtttacaag ttatttgtga agatatgttc ttagtgtttt
      721 gaaatagtat ttgtatatat tcatgcttag ttgagatagt tttgtatgga ttttcatatg
      781 tgtagagtta aatgttaggt actaaagttt atgttaaatt aaatgttaaa gcttatgtta
      841 aaaattttat taaatttaat ctgtaaataa aaaagttaaa agtccaatat taacatttta
      901 cttttaaacc attaaaatct ttattaaagc taacaaagtt ataagttatg agaaatttta
      961 ttgaagttaa tacaaaaatt aattttaaag ctaagaaatt ttttattaga ttcaataaaa
     1021 ctaataaata tagtttagtc aataatgtat taaataaaaa ctttgttaag tctataaaaa
     1081 tatcttaaag gaggtgtaac ttatgaagat aaaagtggag gatataccag ttcaatttca
     1141 tgcaatggta gaaataatcg gaattgataa gttcatagag gttgccaagt tgtatggagg
     1201 aacaaatact tatataccta cgtataaggg gctttttaga tatgctagaa atagggatat
     1261 agtaaaacaa tttaatggag tcaaccacaa tgaattggct attagatata atatgtgtgt
     1321 aagcaatatt aagcgaatac tcaatgaaaa tagtgtttaa atataataaa ataatgttta
     1381 aacataatga aaatagcctt gttaaacgta agagtaaaga caaagaagat gttttataaa
     1441 gtttttttag acaaattaac taaatttact acttattaat ttaaatacat tgtatattct
     1501 ataaaaaaat ttgttaaata ctggagatat attgagaata caaagaaaat ggtcgaaaat
     1561 atttgttggc taaaaaatag ataaaatgat acaattgctt gtataaaggg gaaaaataaa
     1621 taaataaact atataaataa agaaatagca attaatatga cataatatat ttataaaatt
     1681 aagaatataa atagatattt taagggatta gttgttaaat taggaaatat taaaaaaaat
     1741 atatatttat atttaaacta ttttgttatt tatataaaaa tacttatcta gttactagca
     1801 attattagta gaaatggggg aaatagagat gaataaggga ttttacaaat taaattcagt
     1861 ggtattgaac tttgatatta agaatgatgt taacaagata agatttaatc attatataat
     1921 gagaaaagaa aacatacaaa gacttcaagg aactctgcca aacggacaat tttatatgac
     1981 agtgagaaga gctgctacag atttacaact ttctatttct actataagta ggcttgtaaa
     2041 agagtttttg gatttaggga taataagatt agtgtctaag ggtgtaaaag gtaatcgttc
     2101 tgtatatagt tacgtaagta tagagtttaa tggaagttta tttgatgata gtaaatatat
     2161 ggaacttgac tgtgtatgta atgatgaatt tattgataat agtggcatag agtttgattg
     2221 tgatagcaaa gataattctt ttaatggaga tataaatgta gaaactgtgc attgcattaa
     2281 tgagtatttc tctgatgatg atgtagagca taattataac aatttccata acaaagatac
     2341 tataaaggat aaagccattt ctaatattag cactatcaaa aaatatattg atgaatcttt
     2401 tgatacaaat aaatcagaaa attctaactt acaacaggag tcaataaatt tcaactttaa
     2461 tgatacatgc aatgaaatag ataaaagaac gaataatagt atagaaaata atcaaaataa
     2521 aaggcctatg agattgagtg acatcagtct tttaagtgat aaaggtatga caaataaaaa
     2581 gaattacttt aatcataatc atggtataca aaaagggact aaatctgtaa caaagaaaaa
     2641 agaatcctta aataaattta ataaaaaaaa tatacagcaa agcattataa atatgctaaa
     2701 taaaaaatca ggtaagaatt ttaaaatcga ttctccaata agcataaaac ttatcaatga
     2761 aaggctaaag gaaggctatg tcttagaaga tttctacaaa gttattgagg taaaagtggc
     2821 taagtggaaa aataccataa tggaaatgta tataagacca gaaactttgt ttagctataa
     2881 gtttgaatcc tatgtaaatg aaaacatatc tatgaaaagt caaagtaatg ttcacccatg
     2941 taaagaaggc aatagtaatg atagcagtaa ttatatacat tctgattata taacatatga
     3001 tggcagtgtg cgtgagagaa aaaaatatga gaatgtaaga tactggaatc ctatatgtga
     3061 aatttaatta gtttaaaaat gtatttatga taaataagat attaatagag aatagtgagc
     3121 tatagcaaat gatatatcaa tagttggtag taagttatat taagtaagat attactatca
     3181 ggtagtgaat tatattgaac gatatattaa taattggcag taagttatat caaataagat
     3241 attactatag agtaatgaat tatattgaac gatatattaa taatt
//
LOCUS       DAYATN010000230         3180 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.234, whole genome shotgun sequence.
ACCESSION   DAYATN010000230 DAYATN010000000
VERSION     DAYATN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.234"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            455..2590
                     /gene="rnr"
                     /locus_tag="ACGI8A_003645"
     CDS             455..2590
                     /gene="rnr"
                     /locus_tag="ACGI8A_003645"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFH0181605.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1398 a          288 c          541 g          953 t
ORIGIN      
        1 aaaatcaacc tcatttaaca tataaattta cttttattat aagttttata ttataaagtt
       61 gtatttaaaa aattaattga gttatataaa ttttacatga tttgagtata ctaatagaat
      121 aaattattat atagtaatac ttatatgaga tgaatatttt accaatccta tttgatggtg
      181 tataattagg tgataaagag taatttaagc aattatgata aagaataact taagtaatta
      241 taatgatatt tacaaataat ttataaaaca tcataatttg tttgaagtta gtatgacaaa
      301 gcttacatta aatcaaaaat ataaataagt aattttaact tataataata agaaaatata
      361 gaaaattatg tgataattaa aaaatgtttt gtctgaagaa atataaaaga caatataaat
      421 aataatataa tgaaaagtaa aacaggaggg aaaaatgata ccaggactta aagaaagttt
      481 gctaggatta ataaatgaaa gtgcatataa tcctctaaaa aaagaagaat tagcagaaat
      541 atttgatata cattcatcag aaatgccaat gttttataat tttttaaatg aattagaaga
      601 agatggttat atctgcttta ctaaaaaagg aaaaatagta tctcccaatc aaatgggata
      661 ctttgttgga aaatttgtat cacacaaaaa gggttttgga tttgtagaat ctgatgttga
      721 atatacacag gatttattta tctcaagtga caatataaat ggtgctatgc ataatgatag
      781 agttatggca gaaatagtag tacctgcaac tgaagaaaag agagcagaag gaagaatagt
      841 aaaagtaata aagagagaaa taactagaat agtaggtact ttccaatcaa gtaaaacttt
      901 tggatttgta acaccagaca acaagaaatt tactaaagat atatatatac ctaaaaaaca
      961 ttttagtgga gcagtagata atgataaggt agtatgtgaa attacagtat ggccacaaga
     1021 ggatagaaaa ccagaaggca aaattataga gatactaggt caaaaaggtg aaagaggcgt
     1081 agaaatagat tctataataa gagaacatgg tcttccagaa gaatttccaa agaaagtatt
     1141 gcaagaagca gaagctgtag ctgttgagat tcctcaagaa gagataaaaa gaagaagaga
     1201 tttaagagat ttaaacatat ttactataga tggagatgac gcaaaagact tagatgatgc
     1261 catatcaata gaagtattac ctaatggaaa cttcaaacta ggagttcaca tagctgatgt
     1321 tacacattat gtaagagaga aaaataaact tgacaaggaa gccttaaaaa gagctacatc
     1381 agtttattta gtagataaag taattccaat gttgccaaaa actctttcaa atggagtatg
     1441 tagcttaaat ccatttgaag ataaattaac tctttctata tttatggaaa tagaccataa
     1501 aggaaatgtg gttaaacatg aaatatgtga atctgtaata aattcaaaag ctagaatgac
     1561 ttatacagaa gtatctgata tattagaaaa ggatgatgaa aaacttaaaa agacttttga
     1621 acatgttgtt gaagatttta aaaatgctga aattttagct aggattctta tgtcaagaag
     1681 ggaaaaaaga ggagctatag acttcaattt cccagaggca aagataattc taaatggaaa
     1741 aggcgaagtt gtagatataa agccatatga aagaagaata tctaataaga taatagaaga
     1801 atttatgctt ataagtaatg agacaattgc cgaacatttt tactggatgg gaataccgtt
     1861 tgtgtataga atacatgaga caccatcttt ggaaaaaatg gaagaattga gtaagtttat
     1921 atctacattt ggatatataa taaaaggtga taaagaggaa gtacatccaa aagcattaca
     1981 aggtataata gagaaaataa agggcaaaaa agaagaagga gctatcagta ctataatgct
     2041 tcgttcttta aaacaagcta agtactcacc agaatgtagt ggacattttg gattagcagc
     2101 taaattctac tgtcacttta cttcaccaat aagaagatat cctgaccttc aaattcatag
     2161 aataataaaa gaatctttaa ataataaaat aagtggaaaa agacaagaac agcttactac
     2221 tatagtggat tatgcatctg ttcaatcatc cgagaaagaa agaaaggcag agttagctga
     2281 aagagatgta catgatttct ataaggctct ttatatgaag gataaagttg gtcaagaatt
     2341 tgagggtgtt gtatctagcg taacttcatt tggaatattt gttgagttgg ataataccat
     2401 agaaggattg ataagacttg caaatatgaa tgatgattac tatatttatg atgagagttc
     2461 ttatagtgtt ttaggagaaa gaactaagaa gtcatataag ataggagacc ttgttaagat
     2521 aaaggttgaa agtgttaatg tagattttaa agaaattgac tttgagatag ttgaaaaaat
     2581 agaggagtag ataaaacagc taaaataaga gataacaagt tgtagtattt atgatatgta
     2641 taaatatata gtgcacaata ctctataaaa taaatgataa aaataggtaa ttgagaaata
     2701 tctaatggaa atagttaact gaaagataaa taattaagaa atatttaatg aaagtagcta
     2761 attgaaaaat aagtaattga gaaatgttta atagaaagag ctaactgaaa gataagtaat
     2821 taagaaacat ttaatagaaa aagccaactg aaaaataaat aattaagaaa tgtttaatat
     2881 aagtggctaa ttgaaaaata agtaattaag aaatgcttaa tataagtggc taattgaaaa
     2941 ataagtaatt gagaaacatt taatagaaaa agctagctga aagataagta attaagaaat
     3001 atttaatgta aacagataat ttaaaaatac ctaattaaga atagatatta aaaaatataa
     3061 tttcaatata aaaataagtg tatcatttaa aatttaagat acacttattt tttatttaag
     3121 ataacttttc ttttttagaa tttataacaa taggttaata tatttataat taatgtaaaa
//
LOCUS       DAYATN010000231         2956 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.235, whole genome shotgun sequence.
ACCESSION   DAYATN010000231 DAYATN010000000
VERSION     DAYATN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.235"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            72..1199
                     /locus_tag="ACGI8A_003646"
     CDS             72..1199
                     /locus_tag="ACGI8A_003646"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFH0181606.1"
                     /translation="MKGKITMDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEG
                     KDVIAEAQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESK
                     DVSILAAYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQ
                     MLLMGFKNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLN
                     NIKQEVVETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLH
                     SDIAQSKRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIG
                     RTGRAGEEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1576..2760)
                     /locus_tag="ACGI8A_003647"
     CDS             complement(1576..2760)
                     /locus_tag="ACGI8A_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181607.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1128 a          444 c          404 g          980 t
ORIGIN      
        1 aattaaaaaa tttactaaaa atacttacaa atatgatatt attaattggt taataaaaaa
       61 gaaaattttt catgaaaggt aagataacaa tggatttcaa aagcttaggg attagtgaaa
      121 atacaataaa tattctaaaa aaatcaggaa taacaacacc tacacctata caaaaagaaa
      181 gtattaaact aataaaagaa ggaaaagatg taatagcaga agctcaaact ggaacaggaa
      241 aaacactagc ttttttactt ccgatatttg aaaatatatc attagatata aatgatatac
      301 aagtacttat actttctcct acaagagaac tagctattca aataacagaa gaagctatga
      361 aacttaaaga atctaaagat gtaagtattc ttgcagcata tggaggaaaa gatattggtt
      421 ctcaaataaa gaaactaaaa ggaaatattc atatgattat agctacacca ggtaggcttt
      481 tagaccatct aaatagaaaa acaatagacc ttagtaagtt aaagactttt gttttagatg
      541 aagcagacca aatgttgctt atgggattta aaaatgaagt ggaagctatt ttaaaagaga
      601 cttcaaacaa aaagcaaaca ctatgctttt ctgcaactat aaattctcaa gtaaaaaaat
      661 tagcttatag atacacaaaa aatcctgttg tagtttctat tcaaaaagaa gaaatcactc
      721 ttaataatat aaaacaagaa gtagtagaaa ctacagatag aaaaaaatta gatgccttat
      781 gtaaggtact agatgaagat aatcctttta tggcaataat attttgtaga actaagagaa
      841 gggttgacaa tcttgaagaa gctcttgcta taagaggata taattgtcaa aaacttcata
      901 gtgatattgc tcaatcaaaa cgagaaagaa taatgaaatc atttagaaat ttagatatac
      961 aatacttaat agctacagat gtagcttctc gtggtcttga tatatctgga gtaagtcata
     1021 tatataatta cgatttacca gaaacaccag aagactacat ccaccgtatt ggtagaactg
     1081 gtagagctgg tgaagaaggc tatacatgtg cttttattga ccctaaaaat gaacgaatgt
     1141 taagtgaaat agagacggct atagaatcta aaattagtag aagaattata gaattataat
     1201 ttatagattg cttaaaatac caatgccaca caaacactgt aatatataga aagctacaga
     1261 aaacatgtat caatctatac tttgctgtta tactatttta tattaaagaa aaaagaggct
     1321 atccaaagta ggattagtct cttttattgt caaaataatg aaaatatatt tgacttcata
     1381 taaaaaagag tgccttataa actaaaagtc cattttgaga taccctcttt ttatctttta
     1441 ttcttagttt tatattgctt gtttatagtt gtttaatatt agattttgca aataatctta
     1501 ataaacatgc agatatatta tctatagtat tcttatttat agtattctct ttagcaatat
     1561 tttttgctac tacatctaat ttacattttc atttaattcc gctggccatt tagcttctgg
     1621 attttccttt gcaaacttct tattcttata atgtcctagt gtaagaaatg gaattactat
     1681 agtgaataga ccaagataac cacaatatcc atatccatat ttaacaatat tacttaatcc
     1741 tgtaagtgat ataagcattg acactaccat tattgcacaa gctgtaaata cccttctttt
     1801 cattcttgaa gaaaggaaag aaagtgcttt atggtcttca aatctattta caagaccaaa
     1861 tatagtagtt actcctgtag acattaaaca aaggaataat gcaacattat aaattatgta
     1921 tatagtatga tttccagatt gacctgcaac ataaagagta gggagtgttg tttgcccagc
     1981 agcaataatt tctctatacc atcccattag catagttaca gcaagtccta atgcaattgc
     2041 attcataaca aatgaaatta tcattgcctt agtagcttct tttgagttct taagaagtgg
     2101 tcttgaacat cctgcaagtg taggtataac aaccgattga aaacctgcat aaataaaagt
     2161 attaagtatt ggcttgatat agccaggtgt gaaactcgtt ctatttgata atatttcagt
     2221 tatttctggt actttagctt ttataccaac aataaaaata gtaagagtac atactagaat
     2281 agctattgtc attgctgtag ctgctttaga tacaagcata gcaccaaata tagtaaagac
     2341 aagaagtaca actccaataa taaatacagc tacaaaataa tttactccta tactttgaaa
     2401 tactgctgct gcacctgcta taacagcact tacagctatt aaaaccatta gatagtaata
     2461 tatctcccat attatttcaa gttttggata tggtctccaa agctcacaaa ataaatcctt
     2521 ataattccta caattgttat tattatacat tatgataact tctctcatgg tagcagtaag
     2581 tataatcatt gctaaaattg ccataagtgg tgctgtccat ccatactgca catagtactg
     2641 agtttcttga ttccctgtag caaatcctcc tcctgcatgt gaactaaaaa gaacgcttgc
     2701 aacaccaaaa atagctaaca tagagcccgt tacttttgct ttctttacac ttttttgcat
     2761 gaaatcatcc tccctaaaat gatattgaaa gtttttattt taacacttta ttgataaaac
     2821 aataacattt ttgctcatta aattcaacta tatttttcca atgactttac attttcaact
     2881 tatttccacc tttatattaa atatttttta tatatttatt ttatttttta aatagtattc
     2941 aaattcactt tgtttt
//
LOCUS       DAYATN010000232         2955 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.236, whole genome shotgun sequence.
ACCESSION   DAYATN010000232 DAYATN010000000
VERSION     DAYATN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.236"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(34..210)
                     /locus_tag="ACGI8A_003648"
     CDS             complement(34..210)
                     /locus_tag="ACGI8A_003648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181608.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(264..360)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1157..1558
                     /locus_tag="ACGI8A_003649"
     CDS             1157..1558
                     /locus_tag="ACGI8A_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HFH0181609.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            1621..2622
                     /gene="rsgA"
                     /locus_tag="ACGI8A_003650"
     CDS             1621..2622
                     /gene="rsgA"
                     /locus_tag="ACGI8A_003650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFH0181610.1"
                     /translation="MEYHDLYLARIISQEKNFYQAVCVNGVLFAEVSGKLRFEATIQS
                     DFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRKAAGKVNEEQVVAANIDIVFLCM
                     SLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCDNVHRKLQVVNSIALGVDVVVTS
                     SIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINCLMGGEFLNTKEIRIDDDKGRHA
                     TTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKSFADIEAYFGKCHFRNCTHTSEP
                     GCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSEYLAKKEKKFKNISKINKKIRR"
BASE COUNT         1037 a          380 c          517 g         1021 t
ORIGIN      
        1 actaaaaagg gttctctaga aaaccaaatc tatttaatta tacttactta ctaataccat
       61 cacttgatgc aaacaagatt ctaccacatt ttctccgtac tctagtataa aatcatctag
      121 ttgtgtatct ataaaatcac aataatcata tgaactacca atataagcag tcaacatatg
      181 tgtacatcta tccattttat ctctaaacat atatattatt atccccctaa aaaactttca
      241 taaaaacaaa aagaatatct ctttttggca actggctgac ataactcata gtttatttta
      301 taagcgttag tccctatagc tttgcgtcac taaatttctc tagttttgcc gatttagttt
      361 tattctacaa ttacaataat acaatagaat tgcttattat tcaatatact tatttgaaaa
      421 ttaaataaat ttacaaatat gaacgttatt ttatatcaaa aaaaatagac tatgcatgta
      481 gaatatatgg aaaaacttga ttgtaggttt tgtatgaaaa gacagttgtt attaatttaa
      541 gtgagataaa ttgagcaaca ggcataaaat gagaaaacaa ataatagcag ttaattttta
      601 ttattaatac taggtgaaca ttaaatttca aaataccact agattttata gaataagaaa
      661 atgagacaaa cataaatata tgaatatatt aaattatact tgaacatact tatattttat
      721 gataaaattt caataataat tattagtgaa ggagtgtctt gcctgatgtc agagttatat
      781 ataatggtaa ccaacaaata aatagttgga gacgcatatg ggttctgttt aatcagaatt
      841 tttaagtgta tttttgatac atttgattgt tgtgtcttac tctgccttta tagataacta
      901 acattagaca acaaataata atattacata ttactttgtt aaattgtggt gtagtgggct
      961 ttaatctgct acacttttat taattttaca cagtattatt ttgaatataa ttatgttgaa
     1021 atagaagata ctaaattttc tagtccacag gggttttctg tttttgtgta ggtaaatatg
     1081 tataccacat aaaaaggcag atataaatac ctgtggactt tttttgtaga aaatacacga
     1141 tacggaggaa ataaaaatga attataaaaa tagaaaacga gcttattatc aggaaaattc
     1201 ctgtatggat aactttattt gcaaaaattg tggttgcctt gtagttcctg aaggagctgg
     1261 tacacaacat agaaatcatt gtccacattg tttacatagt cttcatgtag atatcattcc
     1321 tggtgaccgt gctgcagatt gtgatgctaa tatggaacct attggagtgt gggtgagaaa
     1381 aaatggtgag tgggcaatta ttcatcgttg tacacgctgt ggacatctta gttctaatcg
     1441 tattgcagct gatgataatt cagtgaaatt aatgtcaatt gcattaaaac cactggcaca
     1501 gccccctttc ccactagaaa gaatcattga aaagttggaa aaggaggaaa cagaatgaat
     1561 ggtatgaaac aaatcaattt acaagactat ggtctaaata gaaattttat tactcaaagt
     1621 atggaatatc atgatttata ccttgctcgg attatctcgc aggaaaagaa cttttatcaa
     1681 gctgtatgtg taaatggtgt actgtttgca gaggtttcag gaaagctgcg ttttgaggct
     1741 actatacaat ctgattttcc cacagttggt gatttcgtta tgttggatcg ttgcaataat
     1801 aaaaatggta atgctattat tcatcatgtg cttcatagaa aaagcttgtt tgtgcgtaag
     1861 gctgctggaa aggtaaatga ggagcaggtg gttgcagcta atattgacat tgtttttttg
     1921 tgcatgtcac tgaataagga ttttaacatt catagattgg agaggtatct gtctttaaca
     1981 tgggaaagtg gagctatccc agtagtagta cttactaaat cagacttatg tgacaatgtg
     2041 catcgtaaat tacaagtagt taattctata gccttgggtg tagatgtagt tgttacctca
     2101 tctatagagc cagatggtta taaacagatt tctaagtata ttaaaccagg ctgtactgtt
     2161 gccttacttg gttcttcagg agttggaaaa tcttcattaa ttaattgtct gatgggtgga
     2221 gaatttttaa atacaaagga aattcgcata gatgatgata aaggaagaca tgccacgact
     2281 agacgagagc tatttttaat ttcaggtggt ggtattgtta ttgacacacc aggtatgaga
     2341 gagttgggaa tgtgggatgt ttcagaaggt ttagataaaa gttttgcaga tatagaagct
     2401 tatttcggaa aatgtcattt tcgtaattgt actcacacct cagaaccagg ctgtgctatc
     2461 tgggaagcta ttgctcatca agaactttcg cctaaaagat gggaatccta tcgtaggctt
     2521 aaaactgaag cagtatttgc agacaataaa agcgaatact tagcaaaaaa agaaaagaaa
     2581 tttaaaaata tctcaaaaat aaataagaag attagaagat aagtgtagtt tttttgttca
     2641 ttctatgagt ctatatacta ttaatatatt aaatttgcca tcaatttgtt actttaatgt
     2701 gattttgatg gcaaatttgg tttgtggtat tatttaaagc aatttattaa tttttttaat
     2761 aaagtttgat tttttatatg atgatatata catgtgataa tgtatacata tttgttacga
     2821 taggctcctt tataaagagg tcttatgtaa gataagaaaa ggcatattta tactaataca
     2881 taatagagta tatgcttatg tatgatagag atgtttctta agagcaaaac tgcaatattg
     2941 ctaatttata aagta
//
LOCUS       DAYATN010000233         2917 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.237, whole genome shotgun sequence.
ACCESSION   DAYATN010000233 DAYATN010000000
VERSION     DAYATN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.237"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            58..1203
                     /locus_tag="ACGI8A_003651"
     CDS             58..1203
                     /locus_tag="ACGI8A_003651"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFH0181611.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIILDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            complement(1317..2693)
                     /locus_tag="ACGI8A_003652"
     CDS             complement(1317..2693)
                     /locus_tag="ACGI8A_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFH0181612.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     regulatory      complement(2787..2889)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         1180 a          475 c          375 g          887 t
ORIGIN      
        1 aaacaatgta taataaaaaa tagttgacta taattattaa aatataagga gtaaaatatg
       61 aatacatttg aacaattaaa aataagttcc actctaatag atggactcaa aaaacaagac
      121 ataacttctc caacagaagt tcagtcactt gttataggga atataattca aaataaggat
      181 ttattgatta attcacagac tggaacagga aaaacactag catacctttt acctatattt
      241 gaaaaaattg atacatcaaa aagagaaaca caagctctta tacttgcacc tacgcatgaa
      301 ttagtaatgc agattacaaa ccaggttgaa ttattggcta aaaatgcaga actctccgtt
      361 acttctttag ctttaattgg ggaggtcaat atacaaaaac aaataaaaaa tataaaagca
      421 gtaaaaccac acatagtaat tggctcttgt ggaagagttt tagacttgat aaaacaaaag
      481 aaactaaaat cacataatat aaaaactatt atcttagatg aagtggacaa tctattgaat
      541 ggaaaaaata taacttgtat agaagacata ataagaacaa cgttaagaga tagacaaatt
      601 ataggatgct ctgcaagtct tactgatagc actataaaaa tttgtgataa acttatgaag
      661 gagtttgaaa taataaaaac aaaggaaaaa tcacaaataa atcctaacat caatcactca
      721 tatttattag gtgagataag ggataaattt acttttctta gaaaggcatt agcagctaca
      781 aatccaaaaa aagctattgt atttgtaaat aatgaaaaaa atatagaagt cctagtatca
      841 aaactaaatt atcataatta caaagcaata ggaatttttg gaaatatgga aaaagaagat
      901 agaaagaatg caataaataa atttaaactt ggtaaagcaa agattttaat tacaactgat
      961 ttatctgctc gtggactaga tattgtggat gtaagtcatg tttttaactt ggattttcca
     1021 aaaagtaaaa atgaatacct gcatagatgt ggtcgaactg ctagaggaaa tcgaagtggt
     1081 aacacaatct caataataac aaaaaaagaa ttagatatta taaaagattt gcaaaaagag
     1141 ttcaatatag taattacacc taaaacttta caaaatggag aactaataga cattattaaa
     1201 taatacaata aatacaaaaa tacaacaaat acaaaagagc tgtagtttat catcacaatt
     1261 gataataaat tacagccctt tattttatat taaattaaaa ttttaaataa tacctattat
     1321 agtagccaaa gttttattat aaataatatt gccagaacaa tcattactac agaaacatgt
     1381 tttttgtctt ctgccttttt agctccaaat atattataaa atatatttac gaaaacataa
     1441 gttaatatac ctaaagttaa accatcacct atactatatg ttaatgccat acaagcaata
     1501 gtaacaaaag ctggaacacc ttcagttata ttatcaaact ctatatcctt aactgtacca
     1561 agcattaaat atccaacata tattaaagct ggtgctgtag cacatgatgg aattgctata
     1621 aatacaggag agaaaaacat tgcagctaag aataaaattc caactgttat tgctgtccat
     1681 ccagttcttc cacctgcgat aactcctgtt gaactctcaa cataagttgt aactgtagat
     1741 actccaagtc ctgcaccaac tgtagttgca accgcatctg taagtaaggc tctacctaca
     1801 ttaggaacat tccctttttc atctaacatg tttgctttag aacaaacacc aactaatgta
     1861 cctactgtat caaaaaaatc aacaaataaa aatgtacaaa ctactactaa aaaaccacct
     1921 atatttgttg gatgtagtac atatcctaaa tctattttac ctgctattgg tgctaaactt
     1981 tcgaatttaa ataatccacc aggtaaatat atacctaatt tttgtgcata ctctggattt
     2041 ataaaagcaa atgcccaagc aagtaatgaa ctcactacta ttccaaaaag tatagaacct
     2101 ttaactcttt ttttatctaa tacagcaata attattaatc ctacacaagt tattattact
     2161 gctggtgtaa atctacccat tttgataaga gtggcttggt ctccaataac aattccactg
     2221 ttaactagac ctataagtgc aataaatatt cctataccac ctgtaacagc taatttcata
     2281 ttttttggta tcgcatttac aacagcctca cgaactccac ttactgataa tactataaaa
     2341 ataatacctt ctacaaatac tgctgtaaga gctacttccc aaggaacacc catttgtaaa
     2401 actactgtat atgcaaaaaa tgcattaaga cccatacctg atgctaaagc aaaaggaagg
     2461 ttagcaacta cacccattaa aatacatcca aatgctgctg ctaaacaagt acctgtaaca
     2521 agtgcacctg caggcatgcc tgtctcagat aaaatatttg gatttacagc tattatataa
     2581 gccatagtta aaaaagtagt tactcctgca attatttcct tcttcatgtc gacattttta
     2641 ttagtcaaaa ttggaaaaag tttctgcata gtactactgt ttgttgtttg cataacaaca
     2701 cctcctatta aattttacaa aaaaacgaca cagtagttga taagctgagc cgtatccttc
     2761 ataattcagt taattaaata tttaattaac taaataatag tatacatagt cagaatattt
     2821 acggtattcc ggtagaaact tatgacccat attgtcaaaa ttatatgtta tacagtacaa
     2881 aacatgaatt tttctttacg aatattattt taattta
//
LOCUS       DAYATN010000234         2858 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.238, whole genome shotgun sequence.
ACCESSION   DAYATN010000234 DAYATN010000000
VERSION     DAYATN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.238"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(312..1019)
                     /locus_tag="ACGI8A_003653"
     CDS             complement(312..1019)
                     /locus_tag="ACGI8A_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFH0181613.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            complement(1822..2691)
                     /locus_tag="ACGI8A_003654"
     CDS             complement(1822..2691)
                     /locus_tag="ACGI8A_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFH0181614.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          992 a          462 c          242 g         1162 t
ORIGIN      
        1 tacctttttt gctttcattg ttaaaactat tttttctatt agtttatttc ttataaaaca
       61 aacatttagt attaaaatat atatcacaca atagtatttg caatttaact ttaaaaaaat
      121 aaaaataccc aactgaccta agcaactcga ttgggtataa ataatctaaa aaatcatttt
      181 tgtataaact ctttatttaa cttacatttt tatttacctt ctactttaat tcaacttata
      241 tttctatgct tcttatactc tcattcaact tatatttcta cttatcttat acttttataa
      301 attcatcaca attatttagt tctcgctatc aaagaaattg tattaaaata ctttccattc
      361 tctacttcca tcattttttt gtcatttata gcatatgaat tatcacaatt tatccaatcc
      421 ttccaagctt catcatgaca atttaaagct tcacatttct ctacaatcac agactccgat
      481 tcagaccaaa gtttattcca ccatgtaatt gaatggaagt tcatattatc ttgccaaaat
      541 ggaatgagtt catctggtat acaatccacg aaatcttctt ttaatccagg cattgccata
      601 gcaagtattc ccccctcttt tacaagaggt gatatatgat tgtctaaaaa tccttctttg
      661 tttccaaaat agtgataaga atcgatactt atcaccgcat caaagaaacc ttctgcataa
      721 ggtagttcat gtgcctctgc ctgtattgga aatattttat catccaattt aaactcttta
      781 aatcttttat agttttcagt tggttctatc cataaatctg ttgcaaatac agtagcatca
      841 aattcctttg ctaaaaatat cgaagatata cccttaccac aacctaagtc taaaatacgc
      901 attccctttt ctagttttat cttacttgtt aattcttcca atatttttat acaattaggt
      961 cccatcatat ttttcatgag aaagtcctta tcgtatttat tactttttgt aaatatcatt
     1021 ctatctttcc tttccctatc agttattatt tattaaattt tatacgtttg attttttatt
     1081 ttttatctgc ttcttatatt gagcaatctc ttttttattc attctttaac agacaatttt
     1141 aatgtgtaat tttatccact ctgtgatagt aactttaatg aataatctta tctactgtga
     1201 tagttatttt agtgagtaat tttatctact ccacagcaat cgctttaatg agtaatctta
     1261 tccactctat gatagttatt ttagtgagta attttatcta ctccacagta atcgctttaa
     1321 cgagtaatct tatctactct ataatagtca ctttaatgag taatcataat ttttatatca
     1381 ttttagatac ttactctaat aagaattcat tttctaatac ttaataagtt ttaattactt
     1441 ccactttaac ctatatacta attaacttat ttaatttagt gcatatttga cactttacct
     1501 tttattatac catttattat cataaatata ataattctat ttttttaaaa taaatattta
     1561 taataatcta aaataaatat ttataaattc taatcaaccg taaataataa tctaaaatac
     1621 atcaaaaaat aaactaaaaa atatcgaaaa ataatttaaa agacatcaaa actaaatctt
     1681 ctaatcagca attaaaagtc tattttctta gtttaaaaaa taaagataga ttttttcaaa
     1741 acactggata aataggtaaa aaaataaaca gttagtaaaa taaattttta ctaactgttt
     1801 atttttattg tttaatatgt cctataaatc ctctaagtaa tattttcctt gcaaaaattc
     1861 tgattctaaa cctagatttt taaaataatc aaccatgccc atacacgcaa atttttctgt
     1921 agaataatga gtagcaccta taacattaat taaattatct tttgctattt gatggaactc
     1981 taatactggt tcaaaatgtt ttaaaggctt agtaaaccca gtaatatata gatttatacc
     2041 aagttcagca acttctcttg ctacaaatgg ataacttcct ccccctgctg caatagcaac
     2101 taaaccatct tttaatacag aatctccata atcatacaat ttcacatcat gaccaacaga
     2161 ttttttaatt aattccttaa tctcttctat tgattttagt gatgttttac atataactcc
     2221 tacctcaatt ttatcgtcat atttacaaaa tggtttgact atttctaaac ccatagcttt
     2281 tgcaaaacta acagaagttg aatagtcact atagttgtct agtggagaat gtaatacata
     2341 aaaagaaatt ctacgatttt tcatctcttc catataagat agtggtatgt cgtgaaatgg
     2401 aaaaccttca tctgatgcta tgtatcccat tgcatgatgt gaaaaaatta aaatatcttt
     2461 ttcatcttta tctataactt gcttaattat ttctttatca ggaaaagtag ttgtgtatac
     2521 tttattaata ttttgagcat tatctaatac taatcctatg tatttttttc taaattctgg
     2581 agtaataaaa tcattttcaa aattcataaa actccaatca tcattaatat ttttaatatt
     2641 aaattctaaa tctaattttt tataaagttc aattgcctta ataataacca ctcctttata
     2701 ataatattga attactacat attaatatag aaatttccga taataaactt tatacgttta
     2761 agtattccta cttcaaatta aaaatattat aaatattcaa atttattaat atattttaat
     2821 attatattta ttttttcttt attaatattt tctttatt
//
LOCUS       DAYATN010000235         2761 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.239, whole genome shotgun sequence.
ACCESSION   DAYATN010000235 DAYATN010000000
VERSION     DAYATN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.239"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            155..1087
                     /locus_tag="ACGI8A_003655"
     CDS             155..1087
                     /locus_tag="ACGI8A_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFH0181615.1"
                     /translation="MGFIGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKT
                     KIYSNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSK
                     KAGALIYSIHPIFAFSNKNTDIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRD
                     KDTSSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNG
                     FTKALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNIT
                     IENAIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1062..1889
                     /gene="panB"
                     /locus_tag="ACGI8A_003656"
     CDS             1062..1889
                     /gene="panB"
                     /locus_tag="ACGI8A_003656"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFH0181616.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIIKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1908..2756
                     /gene="panC"
                     /locus_tag="ACGI8A_003657"
     CDS             1908..2756
                     /gene="panC"
                     /locus_tag="ACGI8A_003657"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFH0181617.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSIFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1097 a          334 c          457 g          873 t
ORIGIN      
        1 tttatgaaaa tggtaagata tttatggatg gtatcttgtt ataagaacta tactttaaat
       61 gtttttaatt attttgtctt atgtaactca tatcctcagg aatgagatta gaaattaaga
      121 tgttgtatca caaagtggag gtgatactat taaaataggc tttattggtt ctggaaaagt
      181 tggaacatct ttggcaaatt attttttatc aaaaggttat tgtgtttcag gcttttatgg
      241 aaggaatcaa ttatctttat tagaatcaac aaatttaaca aaaacaaaga tttacagcaa
      301 tttgaaagat ataatttatg aaaacgatat attatttatt acaacatcag atgattctat
      361 tgaaattata gacaaaaaac tttccaaatt taatttaaaa cataaataca tttgccacac
      421 aagtggctct ttaaaatcta gtatattgtt ctgttcaaaa aaagcaggtg cattaattta
      481 ttctatccat cctatatttg cattttccaa taaaaatact gacataaaaa aaatgaaaga
      541 catttgtttt tccatagaag gtgataatga acaagatgat ttagttattc aaaatttcat
      601 agatgatttg ggaaaccaat tttttataag agataaggat acttcatcca cttatcattt
      661 agctaatgtt ttagtttcaa atttagtatt atctttacta gatataggag taagttactt
      721 tattaaatta ggtttatctg aagaggaatc tttaaaagct atcagtcctt tagtaaaaaa
      781 aaacatagaa aatatacttg ataatggctt tactaaagct cttactggtc ctgtttctcg
      841 tggagatata acaccaataa ggaaacatct atctgtattg gataaaaaag atgaggatat
      901 ttacaaaata ctatccttaa acctactaaa aataattgcc ttaggaaatg acaattcatt
      961 aaaaggtata aaaaatatta ctatagaaaa tgcaatcgaa aatttaataa gtaaatctga
     1021 aaaatacaaa gaaatttaca aacttttagg aggaaaaaac gatgaaaaat accatacaga
     1081 cttttaagaa tgctaaatat gaaggaaaaa aactatctat gcttactgct tatgactact
     1141 caattgcaaa aataatggat gagtgtgata taaatggtat tttaatagga gattcattag
     1201 gaatggtcat aaaaggtgaa gaaaacactc tatctgttac catagacgaa attatatatc
     1261 atacaaaagc tgtaaaaaat ggcgtaaaaa atgccttaat cgtaagtgat atgccttttc
     1321 tatcttacca tgtttctata gaagatgcag ttaaaaacgc tggtagactt ataaaagaag
     1381 gaggagctca tgctgtaaag ttagaaggtg gctcaaacgt tataaagcaa atagaaagca
     1441 ttgtaaatgc tcaaatacct gtaatggggc atttaggatt aactcctcaa tcagtaaatt
     1501 catttggtgg gtttaaggtt caaggaaata ccagtgaaac agcaagacaa cttattgaag
     1561 atgctaaatt aatagaaaaa gcaggtgcat tctctattgt ccttgaagga gtgcctacga
     1621 aaatagctga gatggtaact aattctattt caattcctac aataggtata ggggcaggaa
     1681 ttaactgtga tggacaaatt ttagtttatc aagatatgtt gggaatgttt ggagattttg
     1741 ttcctaagtt tgtaaagcag tatgcaaata taggagatat aataaaagat tcaattaaaa
     1801 attacatcct agaagttaat acaggtgcat ttccacaaga aaaacacagc ttttcaataa
     1861 atgaatctga gttagaaaaa ttatactaat attaaggatg gtacaaaatg cttgtaaaag
     1921 aaattaaatt actaagaaat ataatcaaag attggagaaa acatggctat tctataggcc
     1981 ttgttacaac aatgggattt ttacatgaag gacaccaaag tcttataaaa aaagctgtaa
     2041 aagaaaatga taaagtagta gtgagtatat ttgtaaatcc aactcaattt ggaccaaatg
     2101 aagattttaa tagctatcca agagatatag ataaagattt taaatattgt atggatagtg
     2161 gagcaacagt agtctttaac ccatcaccag aagaaatgta tttaaaaggt aattgtacta
     2221 ccattaacgt ttctggactt acagattttc tttgtggagc taaaagacct gttcattttg
     2281 gaggtgtctg cctagttgtt tcaaagtttc taaatatagt aacacctgat aaagcttatt
     2341 ttggagagaa ggatgcccaa caattagctg taattaaaag aatggtcaag gatttaaaca
     2401 tagatacaga aataataggc tgtcctataa ttcgtgaaaa tgatggatta gcaaaaagtt
     2461 cgagaaatac atatctctct gaggaagaac gaaaatctgc tctaatatta aataagagct
     2521 taagtttagc aaaagaagaa ttagtaaaag gcaatctaaa tccagagaat ataaaagaat
     2581 taataacagc taaaataaat tctgaacact tagcaaaaat agactatgtt gaaattgtag
     2641 atagtgagac attacaacct gttaagcaaa tagaacattc tatacttgtg gcaattgctg
     2701 tgtttatagg aaaaaccaga ctaattgata attttacatt tgagttaaat atatagtcta
     2761 t
//
LOCUS       DAYATN010000236         2690 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.240, whole genome shotgun sequence.
ACCESSION   DAYATN010000236 DAYATN010000000
VERSION     DAYATN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.240"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            215..1813
                     /locus_tag="ACGI8A_003658"
     CDS             215..1813
                     /locus_tag="ACGI8A_003658"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HFH0181618.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(2052..>2690)
                     /locus_tag="ACGI8A_003659"
     CDS             complement(2052..>2690)
                     /locus_tag="ACGI8A_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HFH0181619.1"
                     /translation="GPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGATGSIGP
                     TGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIGNSSSI
                     TVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTTEPGGG
                     GVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT          944 a          387 c          416 g          943 t
ORIGIN      
        1 tttaaataaa agtaaattta attcgatatt tgattgggta aaagtatata taataataat
       61 ttttatatta aatttgtagt atttatgtaa gaaataaatt ttatataata ttttttcact
      121 aaattttttt ctttttacaa caaatactca ctaccttttg atataatatt tttatcaatt
      181 attattatat tcaagttttg aaaggagtat taaaatgagt ttattagttt tcggacataa
      241 aaatccagat acagattcaa tatgttctgc aatttcatta gcacatttaa agaacaaact
      301 aggaataaaa gcaacagctt atgctttagg agatatcaga aaagaagcta aatatgcctt
      361 ggattacttt aaggtagatg cacctgaaat tttagataat gttagaatac aagttagaga
      421 tttaaattat gataaagtag tacctttaac accaacttca tctatattag aggcttataa
      481 ccttatggat gaaaaaaatg ttaaaacact acctgtaact tatgatgatg gaacttttgc
      541 tggtatcatt actatgaaag aaatagctaa aaatctactt catcaacact ttacaactat
      601 acacacatca ctatcaaaca tttgtaaaaa ccttaatggt actattcttg tggactgtgg
      661 tgaagatata aaaggtagag tagtcacttt atcatttgga atggagactc taatagagac
      721 tttaaaagaa ggagacatag ctattgttgg agatagatat gatagtattg aatatgctat
      781 tgatacaaaa gttaaacttt taatacttac taatcataca gagatttcaa aagatttgct
      841 agctcttgct aagataaatg gagtgtctgt tgtttctgtt gaaagtgaca attataaaac
      901 ttctaatgta ataaatcaat gtagtttctt agactctata gaagctacag aaaatttaat
      961 tacattcaaa gaagatgatt atatggatga aataaaagag attatgctag aaacaaactt
     1021 cagatcttat ccaatattag atgatgataa taagttttta ggtttagttt caaaaggtca
     1081 tctattaaac ccatctaaaa agaatgttgt tttagttgac cataacgaat atgctcaaag
     1141 tgctgatgga attgaacaag caaacatagt tgaaataatt gaccatcata aacttggtgg
     1201 tatttctact gatgttccta tgtcatttag agttatgcct gttggatgca atagtactat
     1261 aatttatcaa atgtacaaag aaaataatgt tgagatacct tatgaaatag ccgggcttct
     1321 tttatctgct atattatcag atacattatt atttaagtct ccaacaacaa ctgatataga
     1381 taagaaagct tgtgaagaat taagtaaaat agcaaaagta gacatggaaa aatacgctat
     1441 ggatatgttt aaatgcggta cttctttaga tgaatatact atagaagaaa tagtaaatat
     1501 ggactttaaa gaatttaata tgagtggaca tagagttggt attggacaag tatttacctt
     1561 agacatagac tctatatttg ctaaaaaaga tgagttttta tcttatataa attcgactga
     1621 ttatgacaaa ctaattcttg cggttacaga tataatcaaa gaaggttctt atttaatcta
     1681 taaagcagaa gacagcttaa ttgctaacgc atttggtgtt gaaggtgttc aaggtacatt
     1741 tgcaccaggt gttgtatcaa gaaaaaaaca attagtccct aatttaacta ctgcaatcaa
     1801 aaattttaaa taatataaaa tttttaaata acgaaaactg tctcaaatct atatttgaga
     1861 cagtttttta ttatatatat atatattgat aataaagtta aataaaaatc cataaattta
     1921 aaatttttat gaatatattt tattattatt gttattttat tcattctaaa ataggtaaaa
     1981 ctcaatatat aaaaatactt ttgtcattta aataaagcct aaatagtttt taagattatt
     2041 ttagacacgt tttaagtcat aacagtatca gctattctaa atatcgtcaa agtagccgca
     2101 gttcctgctg attgcccatt tcgtatccct actgttgtag atgaaaaatt atttattgta
     2161 aaagtagtcc caccaaataa aaaaccagca aaatatccag aaagtactcc tccgcctcct
     2221 ggctcagtag taagccctgc caaatctttt cctcctaaaa ttgggaatag gatagcgaaa
     2281 gaatattgat ttatacttga tgaacctggt tgtaatgcta ctgaaactga tatataatac
     2341 acaccatttt cttgtactgt aaacgtacca tttgcatcta ctgttatact tgaactattt
     2401 cctattattg tttgccaacc tgttatagca tctccattat ttagtgtttg tggagatggt
     2461 gcatttacga ggaagttagc aactggctga gaggaattgc ctgttgctcc tgttggacct
     2521 gttgggcctg ttactcctgt cgctcctgtt gctcccgttg gtcctatact tcctgtcgct
     2581 cctgttggac ctgttactcc cgttggccct atacttcctg tcgctcctgt atttcctgtt
     2641 ggccctattt ctcctgtcac tcctgttggg cctgttgctc ccgttggtcc
//
LOCUS       DAYATN010000237         2646 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.241, whole genome shotgun sequence.
ACCESSION   DAYATN010000237 DAYATN010000000
VERSION     DAYATN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.241"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            467..946
                     /locus_tag="ACGI8A_003660"
     CDS             467..946
                     /locus_tag="ACGI8A_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0181620.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            943..1722
                     /locus_tag="ACGI8A_003661"
     CDS             943..1722
                     /locus_tag="ACGI8A_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181621.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFIPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1972..2472)
                     /locus_tag="ACGI8A_003662"
     CDS             complement(1972..2472)
                     /locus_tag="ACGI8A_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFH0181622.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         1037 a          362 c          319 g          928 t
ORIGIN      
        1 gttcatctct atctaaaata atgacatttc taactcttaa ttctcattta agtttcattg
       61 ttttttctta aaagcctact tttagtcaag atactgttat ctttactcat tatttaaaga
      121 acttatttta aatctgtaca tgaagtaaat aatccaaaag cattgttatt ctaactactc
      181 aacattaaat acttactatt ttctaattac tcaatataaa gacttactat aaaactaaat
      241 acataaatgg cttctaaagt ttaatatatc tacatggctt tatatatgac aggaaaaata
      301 ctagaaaaaa tatattatag aatacttgta tataataggc aaaactttca aacttaaatt
      361 tatcaaataa tcataaaaaa agtgaaccat ctatagtaaa attttatcta ataagtgaaa
      421 ggcaaaaaag tagcgctacg atttgcatag aagggaagga acttagttgg ataaaactac
      481 atttacaaac aatatacttg aatcagaaca gactttgtat cgagtatcta agtctattct
      541 aggaaatgac caagactgtg aagatgccgt caacaatgct attttaaaag catatgaaaa
      601 gttggattct cttaaggaag aacaatactt taaaacatgg ctgattagaa tagtcataaa
      661 tgaatgtaat tcattaagac gcaaacgatt aaaatctcta tcatttgaag atgtcttcaa
      721 aaacaaaaaa attgatgaaa aagatgacta tagcgatttg tatactgcta ttcaaagtct
      781 acctaaaaaa attaaaattc caatagtcct ttactatatt gaaggttatt cagtagatga
      841 agttaaggaa atattagata tacctcaagg aactgtaaaa agtagattat caagaggaag
      901 aagattatta aaaactaaat tagaaaacac agaggtaatc atatgaataa aaatatatta
      961 aataattcgt ttccagaagt tccagaaagt tttcataata gtttaagcaa aacactaaat
     1021 agtttaccag aaagagagga aaattataaa atgattaata ataaaacaca caaattacca
     1081 tttagaaaag gcttagttgc aactttagcc attactttga tattgagcac aacagccctt
     1141 gcagtaggac atatatatag tttagttgga aaatcaagta ctaaggctac ctatacatct
     1201 atcccaacta cagaacaagt aaaaaaagac tttaacttta cccctaatat agtttctgaa
     1261 tttagtaatg gatataaatt taaaggagca tatactgtaa ataaaaaagc atttgacaaa
     1321 gaaaataatg ttttaggaaa atcaaaagca cttagttttg aatataaaaa tggcaatgat
     1381 gaaatatctc ttgacacagc aaataatgta cttgggaaga gttctgacaa tctaaaagtt
     1441 gtagatactt acaaagatat taatatttct tatgaagcat tcgcacaaaa gtttatacca
     1501 gaaggttata aaatgacaga acaggataaa attgatgaaa aatcaggcaa atacacattt
     1561 agtgtggggc caaataatga atcagacaaa gttgaagtta atgaatttaa gtttttgaca
     1621 tggaaacaag atggagttta ctattctttt acagttcaag actcaaattt atctatgaat
     1681 gacttagtta aaatggctca tgaagtaata gatgcaaaat aaacataagt aataataagt
     1741 tgtttatgag tttattattt atgactttaa tttttttatt agtaaacatg gttgtatctt
     1801 ccatctttat attttcttta aatttaatat ataaaatttt agatatttag ctaccttcaa
     1861 aagaagcatt attaaaaagc ccttaaaaat atttctttta tatggaattt taagggcttt
     1921 aactttattt tatacaagtg tatatggaat tttaaagact ttaactttat tttatataag
     1981 tgtaagctcc atttgaatta cttcttcttt tacaggtcta tcattccatg taaaaccact
     2041 agatattgta tttacctcta caaaccctaa agatttataa acatgaatag ctctactatt
     2101 acttttaaat acatcaagct caattttttc atacccatat tcttttgata aatcaataat
     2161 ttgttttaaa agactacttc cgataccttt ttccctatac tctttaagaa tacccatgcc
     2221 taaataacca actttttcag ttttaggtaa aacgtcacac caaccaatac agttatctga
     2281 ttctaaatca attataaaca gttgtggtaa gtttttattt ataatatttt ttataaattc
     2341 aactgtttct tcaaaaggaa aggcttcatc catagctaga tatttcctct cttttgctat
     2401 tttatcaaaa gtttgccaat acgaataaat atacttttct tctgccttta taaattttat
     2461 gccttctatc aacttaacat ttcccctttt taatttttga ttgtagttaa cttattttat
     2521 atgattctag tttataatgc tatataaatt actatatatt agcttaaaat atctttgtat
     2581 atttctataa aattaaaacc aatatatcta tctcttgcat ctctattaaa ataattttta
     2641 atagta
//
LOCUS       DAYATN010000238         2642 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.242, whole genome shotgun sequence.
ACCESSION   DAYATN010000238 DAYATN010000000
VERSION     DAYATN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.242"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(165..977)
                     /locus_tag="ACGI8A_003663"
     CDS             complement(165..977)
                     /locus_tag="ACGI8A_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0181623.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            complement(1053..2471)
                     /locus_tag="ACGI8A_003664"
     CDS             complement(1053..2471)
                     /locus_tag="ACGI8A_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFH0181624.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
BASE COUNT          918 a          480 c          272 g          972 t
ORIGIN      
        1 ataataaaaa aataagaggc tgtctaaaaa tatagataag ccttttttca taatcgtaat
       61 tataaaaata tacttgattt catatgaaaa agagtgtctc ataaactaaa atgtttactt
      121 tgagacacca ccttttttat tcttatttat actttgtaga catattataa catgcgtttc
      181 actggtatct gaacttctgt caaccagtct tcttcactct ctttattcca tataccatct
      241 atataagatt ctctaggatt tccaaccact ttatatttat tatcctctat ccatttaaat
      301 gcatctgcgt aagtttttcc taatgttgaa tatggaccat tgtgtaatat acaaactgca
      361 ttttcaactg catctaattc tttaaatttt aatatatccg tatcttcctt taaatcattt
      421 atagcttgac acatttcaac atccatatca gtctctctat attcaccatc atgatatata
      481 ttaaaacaat aatcaggaac tgcacatgta caccctgttt tcatcatttc ttcagccatt
      541 acatttggaa atagattaaa aagttcatca taccccttta acacttttct catagaagca
      601 actttaactt ttggtaaaga cttaataatt acatcattca tattgacttc tcctttcaaa
      661 ttcataaggt aattacgtag tttaattatt ttatattctt cacttttaat tacatccaac
      721 atttcttttt ctttattcat taaaatattt tcaacttctt caggattttc tacagctttt
      781 tttatctcag ctaatgataa acccatttgt tttaaagtta ttatctgatg taatttaggt
      841 aattgctctg ttgtataata cctatatttt gtaaattcat ctacatattc tggttttaac
      901 aaccctatat cttcataata ccttaatgtc tttgtagtta ttttatttat tttagaaaac
      961 attcctatag aaaacatctc atcacctgcg cattagattt gattaattta agtttaaact
     1021 ttccccttag gttaaagtca atacaaaata aattataatt gtattaattc ttcaattcta
     1081 tctccactca ataccaaaat atcccctgtt tttataatgg ttgaaccttt aggtattaaa
     1141 acctgttcct ttctttttat cataactatt aatatatcgt ctggtatatt agaatctatt
     1201 atactcttat tagcccattt attaccctca tcaacattta cttcgataag tttagagcta
     1261 atttctccat cataatcatt aaatgttttt aatacaggtg tattatcatc aactaaatcc
     1321 aactttctgg ctacagttgg tatcaatgaa ccttgtattc ctactgaaag caacgctata
     1381 aaaaatatta tatggaatat gtcattctct atggctactc cttgagtaac agcataaatt
     1441 gcaaaaacta ctgaagccgc acctctaaga cctacccacg atatgaataa tttttcttta
     1501 tttgagaatt taaattttgc tagaactata aatgttgcta atggtcttgc aataactatc
     1561 atgaatatag atatggccat tgctatacca aatacatttg gtaacttaga agggaaagat
     1621 aacaatccta acataaagaa taacattatt tgcataatcc atgaaattcc atcaaagaaa
     1681 tgaaccaaac tttttttaag aggtatccta ctatttccta taataatacc tgaaatgtaa
     1741 acacttaaat atccatttcc tccaagatac tcgctaaatg cataagaaag tattgcaata
     1801 gctgttataa atatagtata aaatccttct atctcgaagt ttgaatgtct taaaatataa
     1861 acagataatt ttgctagtat aaatcctata gctaacccaa aagctatttg ttttattacc
     1921 attggtgcaa taagttctac cccagaatta ttcatcaatg tcaaaagaat tagtgtcagc
     1981 atatacgcta ttgggtcatt acttccactt tcaacttcta ataatgatgc aagtgaaccc
     2041 tttaagttta gtttttgaga acgcaacaca gcaaacacag atgcagcatc tgttgaagct
     2101 gcaattgccc caattaataa accttcaagt aaactagttc ctattaccat gtggcaaaac
     2161 atacctgtaa ccccagccgt aattattaca cccagtgaag acattaatat agaaggtata
     2221 gcaacaggtt tagccatatt ccagtttgtt ccaaaccctc caaaaaacat tataaatatt
     2281 agtccaatag aacataattg ttttgtcagt tcatagttat caaaatagat tcctaccatc
     2341 ccatcagacc caaataacat ccctagtaag ataaaaatca ataatattgg tactccaaat
     2401 ttatacaata ccttgctaga agttatacaa atcaatagta ctaacccact tataatcatt
     2461 aattctgtca tagcatcaac tccatttcat ataaattttg tagattaatt tacatctaca
     2521 attttattac ttattcaaat ataacatgac ataaatttta taagttatgg tacataaagt
     2581 tcaacaaaaa tttcacaata cattaaaatg tgagtgttat acttaaattt atatatttgt
     2641 tt
//
LOCUS       DAYATN010000239         2590 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.243, whole genome shotgun sequence.
ACCESSION   DAYATN010000239 DAYATN010000000
VERSION     DAYATN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.243"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            442..1317
                     /locus_tag="ACGI8A_003665"
     CDS             442..1317
                     /locus_tag="ACGI8A_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0181625.1"
                     /translation="MLVYYLYNLIFLEGGVITLKLKIVGSGGMSIIPSSFCKCKICEE
                     ARQKGGRYERLGPSLYIDDIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGI
                     RIVEKIGCDFITGENNPIGFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKI
                     NNFDIDLINNNPDRNITFYVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDD
                     GILNDGTKLKDTPFKDEIFTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKK
                     LDNIYFAYDGMEIIV"
     gene            complement(1627..2406)
                     /locus_tag="ACGI8A_003666"
     CDS             complement(1627..2406)
                     /locus_tag="ACGI8A_003666"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFH0181626.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT          947 a          374 c          302 g          967 t
ORIGIN      
        1 ttataagatt atatactatc cttatactct agttaaatat attttaatca ctaatataaa
       61 ttagtttttt taacaaaaca ataaaagagt aaagtacaaa taattccttt gtactctact
      121 ctttttaact ttctttgtac aataatacaa tgataaatat aaaaaattat atattccatt
      181 ctatagaaat atgctcacca ataacatgta tgttgttaat caatatatta tatcaaatta
      241 tatcaaaatt acataaacct tataggacaa tcatatctta ttacaagaaa aatgtcttat
      301 ccatattgaa taagtcattc agttcatata caaagtcaat ttcattgaga aacatttaat
      361 atatgtttat aaaaatttca tactattctt tcatatttac atgtgatata atttattaac
      421 tcataatttt gttaatcagt tgtgcttgta tattatttat ataatctaat atttttagaa
      481 ggtggtgtaa taactttgaa attaaaaatt gttggtagtg gcggtatgtc aattattcca
      541 agttcatttt gtaaatgcaa aatatgtgaa gaagccaggc aaaaaggcgg aagatatgaa
      601 cgattaggac caagtttata tattgatgat ataaaaatgc tgatagacac acctgaagac
      661 attgcagtag cttgtaacag acaaaaaata tcggaagtaa aacatatttc aatttctcac
      721 catgacccag accatgttaa gggtattaga attgtcgaaa aaataggatg tgactttatc
      781 acaggagaaa ataatccaat tggcttttat gcgcttcctg aagtaattga agatataaac
      841 cgaacgaatc tcgattgtct taaatattat ggtgatgtta taaattgcat ttctattaat
      901 gaaacttcgc atgtaaagat aaataatttt gatattgatt tgattaacaa taacccagac
      961 aggaatataa ccttctatgt tatcaaagag aatgaaaaaa aagtcatata tgcctgctgt
     1021 aatcccaagc catttactca tagtgatatg tactttgatg ctgatatttt aataataagt
     1081 ctagtttctg atgatggtat attaaatgat ggtacaaaac ttaaagacac accatttaaa
     1141 gatgaaatat ttactttaga tgagattgtt gaaattaaaa actattatag aattaaaaaa
     1201 attattgtta cacatattga cgaaatgtgg ggaaaatcat atggttatta cagtgaatta
     1261 gaaaagaaac ttgataacat ttattttgca tatgatggta tggaaattat agtctaaatc
     1321 attaagtaat aagcataatg caaaataaga aaacttcaat ttaacagtat agtcaaacta
     1381 tgtgatatca acatatcgca gtttttacaa aatatcatag tatttatctt tataaaattt
     1441 caagtcctac atattttaat acttcaatat ctaaataaag taaaatcaaa aatatatttt
     1501 atatacatta tagacggctt atcgtcacta gataagccgt tttgtcacaa ttattatttg
     1561 ctcaacaaaa cttctcataa tatataacag attgaatctc caactgatac ttaattacca
     1621 aaactcttat tccttaatat gttgttgcat taaccaaacc ttcatcatca ttttttgtgg
     1681 caatagtttt gctaataaat gactggattt tgtatttata ccatacacag aaatatcctt
     1741 atttttcttt gcatccttca atgctttttt ggcaacaacg tctggagaca ctatgcctgg
     1801 gaaatttttt gtcccttttt cagctacaac tatacctctc ttaaatagat ttgtactcat
     1861 ccaacctgga catacagcaa ttgcatttat ccctttactc ttaagttcaa catttaatgc
     1921 tcttgtataa tttcttataa acgctttagt tgaactataa atattttgat atggcaatgg
     1981 ctggaatgat gcttgtgaag aaatattcat gatatggctt cccttttgca tatatggaat
     2041 acatataagt cccaatgcta caacagcact aatatttaaa tcaatcatat taatagattc
     2101 atctatactt aaatcattgt atgaacagaa ttttgcaaaa ccagcattgt ttataaggta
     2161 ttttatacat acattttctt tacttaaaaa tgtaccaatt tcttttactt cttctatttt
     2221 tgataaatct tttgaaaaaa tttttacctt acttccaaat tcatctacta attgatttaa
     2281 tctcgccttg tttcttgcaa ctgcccatat ttctgttatt tcttcttcat ttattaataa
     2341 ctttagaaac tctttaccaa aaccactatt agcacccgtt ataattgcaa tcttcttata
     2401 actcataatt attctccttt accataagtt tatacgctca aactgtaaac ttatttttta
     2461 caaataaaac accctctatt cagatgttat attcatatta tttgagatat tatatttata
     2521 tcattttcaa taattgttat atagcataaa aaatagtgtt aatattatag aatcggtatt
     2581 gattatattt
//
LOCUS       DAYATN010000240         2452 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.244, whole genome shotgun sequence.
ACCESSION   DAYATN010000240 DAYATN010000000
VERSION     DAYATN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.244"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            277..>2452
                     /locus_tag="ACGI8A_003667"
     CDS             277..>2452
                     /locus_tag="ACGI8A_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFH0181627.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAVTVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSGGSSSNQTSVNNEAAKITSVPTPAKDATRLTMPSVSSGYTIAIKTSSNE
                     SVIKKDGTIIPPNTATTVKLVFTVTHTSSGKTADTKELSVTVPAKSTDEELQAALDNE
                     VAKITSVPAPAKDATKLTMPSVSSGYTIAIKTSSNKSVIKEDGTIIPPNTEETVTLVF
                     TVTQESSGKTADTGEIDVVVPAKSTDGEIQAEVQAEADKITSV"
BASE COUNT         1065 a          329 c          439 g          619 t
ORIGIN      
        1 ctttttttat acaaaaccct attttttata acaattcatt aacataaaaa tcaaacttac
       61 cttaaaatat aaatttacaa atttttcaat caataagaaa atttctttat tccttacata
      121 atgactgcaa tgtaacaaaa taattacaaa ttaattatga aatcattata gttagaaacc
      181 tctttaaaat tagatattaa gtatccttta gaattagaac gggaacaaag ttaaaaaatt
      241 aagtaatatc aaatggacaa gggggaagaa aataaaatga aaataccaaa gaagattgct
      301 gcaatgctta cagttacaat gatagctgga agttcaacag caggcatagc tagtgcacaa
      361 actgtggcaa caaatttaac agggcaagaa agatatgaaa ctgctgttaa aataagtcaa
      421 gatggatgga aaaatgctga tgaggtagta atcgtaaacg actcatctat agcagatgct
      481 ctatctgcaa caccatttgc aaaagcaaaa aatgctccaa tacttttaac aagtaaagat
      541 aaattaaatg ataagacgaa agcagaaata caaagattaa aagctaaaaa agtatactta
      601 ataggtggaa cttctgtatt aagtacaaat atagaaaaag aaataaaaga tttaaaaata
      661 tcttttgaga gaatctctgg agctgagaga tatcaaacat ccttagaatt agcaaagaaa
      721 ttagatgcta taagtgatgt taagaaaata gctgtagtta atggtgaaaa aggacttgca
      781 gatgctgtaa gtgtaggagc accagctgca caaaacaata tgccagtaat acttgctgat
      841 tctaaaaatg gaacagcagt tgcagacaaa tttataaagg atgctggaat aactcaatct
      901 tatgtagtag gtggagagag ttcaatttct gaagctgtta aaaataaact accaaatagt
      961 acaagactag gtggaacaga tagaaatgat acaaatgcaa aagtaataaa agaattttat
     1021 aaaaagacag atttaaaaaa tgcttatgta actaaagatg gtatgaataa acaagaccaa
     1081 cttatagatg cactagcagt aggtgtatta ggtgctaaaa atcaatcacc agtagtttta
     1141 gttggaaaga acttatcagc ttcacaaaaa tctctagtta actctaagag ttttgataag
     1201 ataactaaag ttggtgggaa tggtaatgag acagcattta atgaaatgaa gtcattacaa
     1261 gaagtaaaaa cagttgaagc taaaacaatt tctgaattaa aatcagctat agataaagct
     1321 actgctaatg atgttataaa ctttaagcca actagtgagg ttaaagaagc atttacaata
     1381 caaactgata aagcagtaac agtaaactta aatggtacat atacaaagac tgtgactatc
     1441 aacatgccta atggtgatgt aaataactat gcaaaagtgg atgatgtagt tatagatgat
     1501 gtaaaagatg gaacttttgt taattatgga aaaataacta acttaaaggt taatgataaa
     1561 aatggagcca agatagaaaa taattctaaa ggtgagattg gtagtttaac agtagcatct
     1621 ggagcaagtc aagtaaaagt gactaatggt ggtaagataa ctacagttac aaataattct
     1681 aaagggacta ctatagataa taaaggtaca ataagtagtg ttaagggaga taattctcca
     1741 acaataagtg gaaatagtcc aagttctaac tcttcaggag gaagttcatc aagtggaggt
     1801 tcttcacatg gaggaggctc atcttcagga ggaagttctt ctaatcaaac atcagtaaat
     1861 aatgaagcag caaaaataac aagcgtgcct acaccagcaa aagatgcaac tagattaact
     1921 atgccatcag tatcaagtgg atatactata gcaataaaaa catcaagtaa tgagagtgtt
     1981 ataaaaaaag atggaacaat aatacctcca aatacagcaa caacagtaaa attagtattt
     2041 acggtaacac atacatcaag tggtaaaaca gcagatacaa aagaattatc tgtaacagta
     2101 ccagctaaat caacagatga agaattacaa gcagcattag acaatgaagt ggcaaaaata
     2161 acaagcgttc ctgcaccagc aaaagatgca actaaattaa ctatgccatc agtatcaagt
     2221 ggatatacta tagcaataaa aacatcaagc aataagagtg ttataaagga agatggaaca
     2281 ataatacctc caaatacaga ggaaacagta acattggtat ttacagtaac acaagaatca
     2341 agtggtaaaa cagcagatac aggagaaata gatgtagtag taccagccaa atcaacagat
     2401 ggggaaatac aagcagaagt acaagctgag gcggataaaa taacaagtgt aa
//
LOCUS       DAYATN010000241         2439 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.245, whole genome shotgun sequence.
ACCESSION   DAYATN010000241 DAYATN010000000
VERSION     DAYATN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.245"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            93..566
                     /locus_tag="ACGI8A_003668"
     CDS             93..566
                     /locus_tag="ACGI8A_003668"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0181628.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(935..1492)
                     /locus_tag="ACGI8A_003669"
     CDS             complement(935..1492)
                     /locus_tag="ACGI8A_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0181629.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            1750..>2439
                     /locus_tag="ACGI8A_003670"
     CDS             1750..>2439
                     /locus_tag="ACGI8A_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0181630.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTL"
BASE COUNT          899 a          321 c          302 g          917 t
ORIGIN      
        1 aatattctaa atttataaaa caaattgtat tgaattgaat ttatttaaga tatttaatta
       61 taattaaaat aataataaag gtgttgttta taatgttcaa agaatactta ataagagaat
      121 taaaagaaga tgatattaaa aatgcattag acttaatctg gaacgtattt gaagaatttg
      181 aatctccaga ttattgtgat gaaggaatta cgacatttca tcaatttata gaatataact
      241 ctattattga aaaaatcaaa gaaaaaactc tcttcttttg gggttgtttt gactctaaaa
      301 aacttattgg agtcttagct attagaaatt taaatcatat atgtatgtta tttgtagata
      361 aaaactatca taaacatggt gttgcaaaaa aattaatgac tacaggcata caatactgta
      421 tttgtaataa atttaattta actgaaataa ctgtaaattc atcaccttat gctgttgaag
      481 tttatcataa attaggattt aaagatactg ctgttgagca aattgttgac ggtatacgtt
      541 ttacacctat gaaattgtta atataataaa ttaaataaaa agcaaagtct cttaaatatt
      601 ttatagttac tttgctttta aaccaagatt taaaataata ctatcttttt tgttttttaa
      661 taatatactt taaaatcctt actaatatga actataattt attttttgat atcctttttg
      721 attaacatat aaattaattc aattattaaa cataaactat gttgtttctc tttatatctc
      781 ttctttgtaa acaatattgg gcatttgaaa ataaatattc ttaaaatata ctgttctcct
      841 gttgattttc ctttggtaca aatgataaaa atttaattta catgccccat aatgaagtgt
      901 tcaataaata agttaaaata atttatttac ctccttaatc atatttttta tgaataccta
      961 ataacacagt atcaatagaa tcggctaaac cttttatagc ttcttcttta ctcaaaagtg
     1021 atttcccaag ttgcaaagtc gaataaccat gaagtacact cattattaag tttaatactt
     1081 cacctgtatt ctttgtattt agattacaag aaagaataag tttttctaat aaggatttat
     1141 attcatcaaa gatttctaat gtttcattat ttccatgcca agtagcccac tgaattgttt
     1201 cataaacacc aggattagaa attataaaat taaagtatgc tatacttata cactttatag
     1261 ctgtatctcc aaaatttcca atagtcgcct ttatcatttg actattcatc tctctcattc
     1321 ctttatgagc tacttcaagt aataaatcat ctagactttc tatatgattg tacaaagatg
     1381 gtgtacgtat tcctagcttt tctgcaataa cttttaaaga aactttgtta agcccatctt
     1441 tattaactat atctgatgta gcttgtataa ctatttctct actaacacgc atgattttcc
     1501 ctctttcatt taaaattaat tactttatta gtttagctaa tctaattagt ttcgtcaatt
     1561 attgtaattt ataaaattct ttatactatt ataaaatctt gacagtttaa ttataattta
     1621 atataatact aattgaatta gttttaatac taatataatt agtattatat taaattctat
     1681 attacaacat tattttatag tttgattaat ttaacaatca ctaatatatc aaactgaaag
     1741 gagattttta tggaaaaaaa atatttcgaa attcatggag ttccttcaat cctttgggga
     1801 aatccttcaa gtaaattata tatttacata catggacaat gtggcaataa agaggaggct
     1861 gaagcttttg ctaatattgc agttccacat gagtgtcaag ttttaagtgt tgatttgcct
     1921 gaacatggag aacgtaaaag agaaaaaaat tcatttaacc catggcatgt tgttccagaa
     1981 ttagtatcta ttatggaata tgcaaagtgc cactggaatc aaatttcgtt atatgcaaat
     2041 agcattggag catggtttag tatgttaagc tttgaaaaag agaacttaag agagtgtatt
     2101 tttgtatctc caattctaga tatgcaacag ctcatttcaa atatgatgct ttgggcaaat
     2161 gtatcagaag aacagcttaa acatgaacta attatcccaa cttcatttgg gcatactctt
     2221 tcatgggaat atctacaata tgtaaaacaa catcctatca ctcaatggaa tgtaccaaca
     2281 aaaatattat atggtaaaca tgatgaactt actgaaatcg gtgttgtaga gaaatttgta
     2341 gaaaagttta attgcaacct tactattata gaaaatggca aacattggtt taacacacca
     2401 caagaattaa attcattata tgaatggtta aatacttta
//
LOCUS       DAYATN010000242         2391 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.246, whole genome shotgun sequence.
ACCESSION   DAYATN010000242 DAYATN010000000
VERSION     DAYATN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.246"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            207..581
                     /locus_tag="ACGI8A_003671"
     CDS             207..581
                     /locus_tag="ACGI8A_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0181631.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            578..1441
                     /locus_tag="ACGI8A_003672"
     CDS             578..1441
                     /locus_tag="ACGI8A_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181632.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1441..2079
                     /locus_tag="ACGI8A_003673"
     CDS             1441..2079
                     /locus_tag="ACGI8A_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFH0181633.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT          907 a          239 c          335 g          910 t
ORIGIN      
        1 caatttattg gcaggatttt tgaacaatat ttataaattt tttaaaaaaa tttaaatcta
       61 tttaatatgg cagactttca taaaatacat tacatttatt aaatgtataa tattggtaat
      121 tagttttttc taaaatcagt tgacatatta tatatatatt tatataatgt gtatatacta
      181 tatatacaat ttaaggaggt gtcttattga atatagttat tagtaattcg agtgggaagc
      241 caatatatga acagattact acacaaatta aaagtatgat tataagtgga gagttaccag
      301 aaggctcagc tcttccaagt atgagactat tggcaaagga acttagaata agtgtaatta
      361 ctacaaaacg agcatatagc gatttagaac gagacggatt tattgagaca gttactggta
      421 aaggcagttt tgtatctagt aaaaacatgg attttgtcaa agaagaacaa tttcgtttaa
      481 ttgaagaata cctacaaaaa gcagttgata cagcaaaaag tagtgatatt acatatgacg
      541 aattaaaaga tattttattt ttattatata aaggagaatg ataagtatgg atgcaataac
      601 tataaaaaat ttaaataaaa cttataaaga ttttagccta caagatatat ctttttcagt
      661 accaaaaggg agtgtaatgg gatttgtagg tgaaaatggt gcaggaaaaa caactacatt
      721 aaaagcaatt ttaaatttaa tatcttatga tagtggaaat atagaaatct ttggtcttga
      781 tagtaaaaaa aatgaaaaag aaattaaaga gcaaattggg gttgtatttg agggaagcaa
      841 ctttcatgaa aatttaaaca ctgaccatgt ttcaaaaatc atgagtaaaa tctataaaaa
      901 ttggaatgac acattattta aagactactt aaaaaaattg agagtaccag acaataaatt
      961 aattaaggag ttttcaaagg ggaataagat gaaacttagt attgctgtag ccttatctca
     1021 taaaccaaaa ttattaatac tagatgaggc aactagttca cttgacccta tcgttcgtga
     1081 agaaatacta gatatatttt tagattttat acaagatgaa gaacactcta ttatcttatc
     1141 ttcacatatt acaagtgatt tagataagat agcagactat attacattta tacataaagg
     1201 aaaaattgta tttagtgaaa ataaggatga attaattgat actatgggag ttttaaaatg
     1261 taaacctagt gattttgata acctttcaag agaggattat tcatattatc gtaaaagcca
     1321 atttggatat gaagtacttc ttaaagataa gcatagattt atctcaagac atccaaactg
     1381 tattgttgat aatacgtcta tagaagaaat tatgttattt tatgtgagag gtgataaata
     1441 atgaaaggtc taattttaaa agatttactt aatttgaaag gaaatataaa gtttatactt
     1501 ttattcatca ttatgtttgg ctttatgtct tctttaggag atggaaatgt taataacttt
     1561 ataggtgtta ttatagtttt atgtactaca atgattgttt caacattttc atatgatgat
     1621 ttgaataaat gggatagtta tgtacttaca atgcctatta atcgcaatga tattgtatta
     1681 agtaaatatt taactatgtt aatatttagc tttataggtg ttcttgtttc acttattgtt
     1741 agtgtaacta ttggttattt taaaaataca cttatattaa atgaaacact acttattaat
     1801 gcactaatct tatcaatttc agtatgtttt ggtagcctta ttctaccact tatttataaa
     1861 tttggtacag aaagagctcg tttattaatg attttatgct ttttagttcc aacactagct
     1921 ttattagttt ttaaaagtat tttagaaaat attagttcac ctatttctat agaaattata
     1981 cttaatacac tagtatattc tcttccattt gttgctattt tactatttgt gatttcatat
     2041 tttatatcat caaaaatcta tagtaaaaaa gaagtataaa atatactata tatgtttctg
     2101 tatttaagta tttaaataaa atactatcga gtttattttg gtagtatttt attttttaat
     2161 tattaatctc tactaggtat ttattatttt attacttata tttaataaga ctatttacta
     2221 ctttctaatc ttatttatat aatttattca ttttataaat tgaactacta atgatattat
     2281 tgaacatagc aatagaataa catctaaaat agtttttatg tattaaatta acttatttta
     2341 atattcaaca atttaggttt actaaattta ttgatgattt ctttttatat a
//
LOCUS       DAYATN010000243         2380 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.247, whole genome shotgun sequence.
ACCESSION   DAYATN010000243 DAYATN010000000
VERSION     DAYATN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.247"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(145..996)
                     /locus_tag="ACGI8A_003674"
     CDS             complement(145..996)
                     /locus_tag="ACGI8A_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFH0181634.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1045..2208)
                     /locus_tag="ACGI8A_003675"
     CDS             complement(1045..2208)
                     /locus_tag="ACGI8A_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFH0181635.1"
                     /translation="MFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKLSR
                     FIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNPYD
                     YLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKNDEN
                     NYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVIDRK
                     DEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPKSF
                     IDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFKEK
                     YNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
BASE COUNT          735 a          432 c          249 g          964 t
ORIGIN      
        1 tatatatgta atactttacc aataaaagct gatatttttc atcaattaaa tgaaaaaaat
       61 acaccattta catataatta atataaattt ttcatataat aaatatccta aacttatcta
      121 aaataaattt aggatatttt atttttatac tctatttaca gacccacaca ctttaatctt
      181 ttctttcaca aaatccttta ctgattgaag ccctgctaat ccatactttt tagggtctat
      241 aacctcagaa tcttcatcta aaactttttt aaccccttta ctatatgcaa ttcttaattc
      301 agttgcatag ttaacttttg caatacctct ttttatagct tcttttataa tatcatcatg
      361 tattcctgaa cctccatgca atactaaagg tattgaaact acatttttaa tttctgataa
      421 cctatctaaa tctattttag gttcaccctt atacaatcca tgtgcagtac ctatagcaat
      481 agccaatgaa gatattcctg ttctatcgac aaactctttt gcctcttttg gatttgtata
      541 atcattacta ttcccgccat ctaaatcatc ttcttttcca ccaactttcc caagttctgc
      601 ttccactggt ataagagaag gactacatgc ttttactact gatttagata tagatatatt
      661 attttcaaag ctttcatgag aaccatctat cattattgat gtatatccac ttcttaaagc
      721 tttcattgca aggtcaaaac ttgaaccatg gtccagatgc attgctacag gtatatttac
      781 tctttctgca gcaacctttg catttgctag ataataatca agacctgcat attttacagt
      841 agatggagta gtttgtaata ttactggaga atttaactcc tcagcagctt ctataacagc
      901 aattaccatt tccatatttt ctacattaaa tgctcctact gcataccctt catcttgtgc
      961 ctttttaagc attgatgttg ttgttactaa tgccacattg ataccctccc aaattattta
     1021 gttataatat tttaatctta tcttttaata ttcatatatt gttacacctt ttactactcg
     1081 atttactgtt cctgtagctg atggagtatc tggcttattt ccaaccttta ctgatacaaa
     1141 caatgctata gtttgtgcaa acaatatgta tggcattgca agatacacat ctggtaattt
     1201 attatacttt tctttaaact caaactttgt tcctgagaag ttattttcat tttctactga
     1261 cactgcacaa gtgaatttag caattttatc cctttttatc tcctctaaaa catctaaatc
     1321 atattttctt gtatataaac agttagatac aaattcaaat acaagtgtat tttcatcaat
     1381 aaatgatttt ggtccgtgtc taaaccccat aggagaatca tacactgtag atatttttcc
     1441 tgctgtaagt tctaaaagtt ttagttgtgc ttcttgtgtc aaaccaccta atcctcctga
     1501 acctaaatat acaactctat caaaatcttt atttattaac tcatgtattt catcttttct
     1561 atctataaca tttcttccca tttcaattat tgcatttata tatgactttt ctacatcatc
     1621 ttctaaacta tcgaatatta acatagcaga taacatcata cacgaaaaac ttcctgtcat
     1681 ggcaaagcct tcatcatttg attttgatgg cattaataac aagtaattat tttcgtcgtt
     1741 tttagtcatc ttagccagtt ctccttctgg attacaagtt atagctaaat gataaaaatt
     1801 atctacaatc ttattcccta aatttaaagc tgctaaactt tcaggactat ttccacttct
     1861 tgcaaatgaa attaataatg ttggtatatc tttttttaag taatcatatg ggtttgaaac
     1921 tatatccgta gtaggtattg cttcaaatat gtattttctt atgtcatttt tatttttaag
     1981 gtaaggaagt attgaatttc ctatgtaagc tgaagttcca gcacctgtaa atataactct
     2041 aaactgccca tgattattac ttattgtatc tatgaatcta cttaattttt ctttgttaga
     2101 tttatatatt tcataagttt ctaaccataa ttcaggctgt tgttttattt cattagttgt
     2161 tattattgca cctaaatctt ttagtttttg ttcttctaat ttaaacattt tcattctcct
     2221 ttcatacttg taacgtttac atttggtaat gaggttataa ccactttaaa ctataaaaaa
     2281 atatactctt ttataatttt ttgcttagat tttttagtac tacttaaact cactataaaa
     2341 tatacactat agattttaat taattttata gtttatcttt
//
LOCUS       DAYATN010000244         2324 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.248, whole genome shotgun sequence.
ACCESSION   DAYATN010000244 DAYATN010000000
VERSION     DAYATN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.248"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            241..2253
                     /locus_tag="ACGI8A_003676"
     CDS             241..2253
                     /locus_tag="ACGI8A_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0181636.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         1035 a          223 c          336 g          730 t
ORIGIN      
        1 gtgtatatta taatttaatc gaatagaaat gaaaaatata catacagttt aagttttaag
       61 caacaattag ggggatttgg tgacaagatt tttaattata agaaagaaag tgtcttaatg
      121 caacttttat aaagtcatta tagacacttt cttttttatt tgataaattt aaattatcaa
      181 aattatattg ctttatacaa ctattttgtt ggaaactatt tattatataa ggagaaataa
      241 atgaaaaaat cagtagctct ggtaaatgat agtagaaaag atttaataga ctttttagaa
      301 aataatttga aattagtttt tggagattct ataaatatta atagatactt cataaatgaa
      361 ataaatgata atgatattat aaatgatgat gtgatattag taatgtcagt agaaagatta
      421 gacaaaatta taaataatat actagataag aaaaaagtga ttgtggtaag aagaactttt
      481 agggaagata aaatttataa tctactatct ttaccacagg gtacaaatgt tctaatcgta
      541 aatgattctg atgaaactac tctagaaact ataagtttat tttataaaat tggagtgact
      601 aatattagac caattccata tatgaatgat aataactata aaaatataaa aatagctata
      661 actccaggag ttccagaaaa agtacctagc tttatctcag atatatttga tttaggtcac
      721 agatatatag atatttctac atttatagaa ataataaatt tattacaaat tgattcaaag
      781 gaaatacaat ctaatctagt taaatactca gaagaaataa taagtttaga tactggtata
      841 aaagataaat ataaggaatt atttttaaaa atagaagaat tagatacaat attgaattta
      901 tcaaaagatg gtatattatt tacttcaaaa gatggtgaaa taaatacata taatagtaaa
      961 gtaaaagata ttttagacat aaatgaggat atatatggaa aatacattga ggatatattt
     1021 gtagatagtt taaaagtttt attgagtgag aaagagatac tagataaagt tgtcgtattc
     1081 aataagaaat atataaatgt aaataaaaaa aacatataca atagagatga aaaaatgggc
     1141 acatattata gtttacaaga aataacatac ataaaaaaat tagaacaaaa tctcactaaa
     1201 aaattaagag aaaaaggaca aatagcaaaa tatacattta aggatataaa aacaaatagt
     1261 cctaaaatgt ttgaatgtat agatttagct aagaaggttt ctaaatctga cttaagcata
     1321 cttatcagag gtgaaagtgg aactggtaaa gaactcatag ctcagtctat acataacaac
     1381 tcaaatagaa aaaatcaacc ctttatagca gtaaattgtg cagccgttcc tgaaaattta
     1441 ttagaaagcc agctattcgg atatgacaag ggaactttta ctggaggtct aaaagatgga
     1501 aaacaaggat tatttgaact tgctaataat ggaacaatat tcttagatga aataggcgat
     1561 atgcctttag aattacaaac aaaactactt agagttcttc aagaaaagca gataatgcct
     1621 gttgggtctc acaatgtaat taatatagat gttagaatca tatctgctac aaataagaat
     1681 ttagaacaaa tgattgataa tagccaattt agagaggatt tatattatcg attaaataca
     1741 attccaataa atatacctcc tctaagagaa agaaaagaag acatattaat tattatggaa
     1801 gatttaatta ataagaaatt agtaatcaca ccagaagcaa aaaaacttat acaaaactat
     1861 atgtggaaag gtaatataag agaactccaa aatgttacat cctatttaaa cattatgtgt
     1921 gaagatattg tattagagaa agatttacct ccaaacttaa ggtcttcaga taacaaaaat
     1981 acatcattaa aactaaaata tagtaagaat gatatattga atatattaga gatactaatt
     2041 ttaaataagg aatcagatgt tggtattggt agaggattaa ttttaaaagc actactcgat
     2101 aagaaccttc aaattacaga aggaaaaatt aaaaaaatat ttgaatatct aaaaaaagaa
     2161 gaacttataa tttgtagttc aggaagatat ggaagtaaaa taactcaaaa aggagaagat
     2221 ttttacaata aattaaaata taaaggtcta taaaattata atttatcaaa caaagaaatc
     2281 tattcaggat taaagtactc cttcaatagg tttttttatt tttc
//
LOCUS       DAYATN010000245         2317 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.249, whole genome shotgun sequence.
ACCESSION   DAYATN010000245 DAYATN010000000
VERSION     DAYATN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.249"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            1069..>2317
                     /locus_tag="ACGI8A_003677"
     CDS             1069..>2317
                     /locus_tag="ACGI8A_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0181637.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIMLEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGK"
BASE COUNT          905 a          294 c          316 g          802 t
ORIGIN      
        1 atattttaaa ggataaaatg ctgtataaat tattggggta ttttacctat acttgttaca
       61 aatttacata ccacttagtt aatataaaac aaatttcaaa gagattggac aacgaataaa
      121 aattgcaatt tatataccat atagttaata taaagcatat ttgtgtcgat atatagttct
      181 aactaagtat aagatttaca tacctcatag ttaatataaa acacatattt tacatgtttg
      241 aagattttat cagaaaccct atttacatac cgcatagtta atataaaact attaatagtg
      301 ctatgttgtt tatttgcatt aacaagattt acataccaca tagttaatat aaaacatgtt
      361 tagcaggagt tatgatgata aaacacatta tttatttaca taccacttag ttaatataaa
      421 accccaaaat aaactaagta ttttcagtac ttacacacat acaactctct caaatttgca
      481 gtgaaccatg agtagtgcaa ttgataacat ttatcataca ccctcaatcc cttacattgc
      541 aactgttaaa ccctacttta tcaaaaatat cgctcactgc aaaatttata tatttttatt
      601 atatcataaa attcaaacta taaaattaaa atggaataat ttatcaattt aagaacacct
      661 acatatttgt aaatacttac tatattatct attctaatag ttcttcttct agctagaact
      721 aatataaaaa tcatctctga aagattagga catactaata taaaaataac tatgaataga
      781 tattttcttg ttttaaacga tatggataaa aaaacttctg aaaatataat caaagttctc
      841 tttaaataac attccatttt atgtaagtag gcattatatt tttcttaaat gtcacttaaa
      901 tgtcagttat atataataat agtaaacaac tgtatttcac taatatttgc tattatctaa
      961 tagtatttat agtttagatt tgatataata catgtggtaa aattttgatt tgtaaaacaa
     1021 aattttacct acatatatta aaacaaattt aaaactaagg agacctatat gttaaaagta
     1081 ctaaaaggaa atataatttt tacaaaaact tcagaaactt tcacagtatt tgaaaatagt
     1141 tatattatgc tggaaaaagg taaagtaaag ggaatattta aagagatacc taaggaatac
     1201 aaaaacctaa aagtagtaga ttacactgat aagcttataa tacctggtat gaatgacctc
     1261 catgcccatg cctctcaatt taaaaatctt ggaatggcta tggataaaga gcttttgcca
     1321 tggcttgaaa cttatacatt tccagaagaa tcaaattata aagatataga atatgcaaca
     1381 aaaatgtata aaaaatttgt aaaagaactt tggaaatctg gaactacaag aatagcagtg
     1441 tttgcaacag tacataaaga agcctctatg aaacttatgg atatattcaa agaagttggt
     1501 cttggagcat acgttggtaa agtaaatatg aatatgaatt gccctgatac tttattagaa
     1561 aacactcagg attctattac tgatacagaa gaaattataa ataagtatag tgatacagat
     1621 tctattgtaa aaccaataat aacacctaga tttataccaa gctgtacaag tgaactttta
     1681 aaaggtttag gagatttatg tttaaaatat aatattccta ttcaatcaca cttatctgaa
     1741 aattatgatg agattgaatg ggttagaagt ctagaacctg aatctaaatt ctatggagat
     1801 gcatataata gatataattt atttggtcaa actcctactt taatggctca ttgtgttcat
     1861 tgttctcagg atgaaataga tttaatgaga gaaaataatg ttatggcagt tcactgtcca
     1921 gcttctaact ttaatgttgg tagtggtgct atgcctatca gaaaattaat cgataataat
     1981 attagattag cacttggatc agatataagt ggtggacata cattgtcaat atttaaagca
     2041 atggtatctg caatacaatt atctaaatta tattgggtta actctggtaa gaagtataat
     2101 ttcttatctt tatctgaggc tttttatatt gcaaccaaaa gtggtggaag tttctttgga
     2161 aaagttggta gctttgaaga aggttatgat tttgatgcat tgattattga tgattcaaat
     2221 ttaaatcatg acaattattc tatacttgaa agacttgaaa gatttatata tgttggtgat
     2281 gatagaaata tcattcatag atatgtatgt ggaaaac
//
LOCUS       DAYATN010000246         2306 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.250, whole genome shotgun sequence.
ACCESSION   DAYATN010000246 DAYATN010000000
VERSION     DAYATN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.250"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            872..1534
                     /locus_tag="ACGI8A_003678"
     CDS             872..1534
                     /locus_tag="ACGI8A_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181638.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1543..>2306
                     /locus_tag="ACGI8A_003679"
     CDS             1543..>2306
                     /locus_tag="ACGI8A_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181639.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLK"
BASE COUNT         1044 a          199 c          361 g          702 t
ORIGIN      
        1 ttgaataaat taaaaatata atgaaagttt agttttaatt catttattta ataaacattt
       61 caagtaaaat aatgtttaaa tcagaaatat aaataaatat tattataatc gttattaaag
      121 accaaaaatc ccaccttaga agtttgtatc atatgaaacc tttatagatt atgaaagcaa
      181 taatatagtg tatgatatat aaaataagtt ataaaagtat ttttttagat attcaaaaag
      241 acattgtaag ttatattaat aatatgggta tcaacgaatt ttggatataa tgagtttttc
      301 aacttaaagt tatgtaataa tgaatgtaaa gaatatttaa tgaaatcatt ttgttggtta
      361 tgtctagctg aatacaataa tcttttagat aaagatttta tatttgaaga taggtgtgaa
      421 aaaattaaga agatattaac atcagcagtt tagaggcaaa gcatgtaaag taaagtctgg
      481 gtaactaaaa gtagtaattt agattgtaac tattgttata aatatgaaaa tgaggaaaca
      541 ggaaatataa gttaggatgt agaaaatagg ttactaaata aaaagcttaa ttatataatc
      601 aaagaaatag gtatatactg tacaatttca tggtgaagaa cttttcctaa ataaaaaggt
      661 tggtaaaaga gatataagaa ataaaattca attataagat atggtatgat aactaaaggc
      721 actatatgga gtgaataggc ggaagaattt tttgaaaatt ataaaaatag tttctgttgc
      781 tacatatcaa taagtataga tagagattgc aaaatccata gcaaaaatcg aatttataag
      841 tgtaaataac atgtaagaca aggagtaaat tatgagtatt ttagagataa aaaacctaaa
      901 taaaatatat ggtaaatcgg aaacagaggt gaaagcgtta agtcaaataa atctaaaaat
      961 aaatagtgga gaatttgttg tgatagtagg taaaagtgga agtgggaaaa gtactttact
     1021 acacataatg gcaggacttg aaactccaac aaatggtgat gtgattattg ataatataaa
     1081 tatatcatct cttgatgaaa agaaaagatc tgcattacgt aaagaaaagt tgggattaat
     1141 atttcaatca tataatttga ttccagtttt aactgttgaa gaaaatataa aactaccaac
     1201 tataaatata aaaaataaag atgaagctta tattaatgaa ttgatggagt tactaggaat
     1261 aaaagataga agaatgtatt tgccaaatca attatcaggt gggcaacaac aaagagtagc
     1321 aatagcaagg gctcttgtaa ataaaccatc aattgtattt gctgatgaac ctactggaaa
     1381 tttagacaca aagacagagt cagatgtatt aagtttatta aaagaatctc aaaaaaaata
     1441 taatcagaca ataataatga taacacataa tatagatata acaaaatatg ctgatagagt
     1501 tatagaaata gaagatggat ttataagaga gtaggtagat ttatgaaact taattatatt
     1561 gtaaaaaaat atataatggg aaataagaaa aatacattat taataattgt aagtattgta
     1621 atttcaacag cgttgttttt aattatgaat ataataagtg aagatgcgac aaacttaatg
     1681 ataaatcaag ctaaaaatga atttacatta aaacatgcac aatatatcaa tccaaaagac
     1741 gaagaaataa aatatataga aaataacaca agtatagata aagttggaaa atcaatgctt
     1801 ttaggaatta gtgatattgg taaaggtcaa acactacaaa ttctatctca ggataaagtt
     1861 gctgaagaac taaatgatat ttacacacta gaaaaaggta aattaccagt agctgaaaat
     1921 gaaatcgcta tagattcatg gtatattaaa caaaaaaaga taaaaaaccc tataggaaaa
     1981 aggataacat tagattatag aagacaaggg atagatcaag aagatttata tactggggaa
     2041 aaagaattta aaataactgg tatcttaaat agtaatccta ttttaaaagc tcaaggaaca
     2101 tcaattggcc ttataagtaa cgattgtgct atcaagaata ttcctactaa aaataaatat
     2161 gatcaagtta tgtttacttt taaaaaggaa aaaaatatac agagacaatc acaaaaactt
     2221 attaaaaatg gaaatttgaa tgagaataac atttatttta ataatgaatt attaatagct
     2281 atatctgata gtatgaattt aaaaat
//
LOCUS       DAYATN010000247         2251 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.251, whole genome shotgun sequence.
ACCESSION   DAYATN010000247 DAYATN010000000
VERSION     DAYATN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.251"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(2..472)
                     /locus_tag="ACGI8A_003680"
     CDS             complement(2..472)
                     /locus_tag="ACGI8A_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0181640.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            complement(1206..1595)
                     /locus_tag="ACGI8A_003681"
     CDS             complement(1206..1595)
                     /locus_tag="ACGI8A_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0181641.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          700 a          396 c          148 g         1007 t
ORIGIN      
        1 atcattcctt actattttca cactgcttta caaatttaat tggatgttga attattttag
       61 aagtttcttc tacagagtat ccctcagata aatatcttct agctataact tccatagact
      121 ctcctatctc aataatatgc ttatccgggt cataaatacg cactactctt tgttgccatt
      181 catgttttag tggcttatgg acaaactcta catctttata ataattgagt ttttcaataa
      241 attcgtcaaa atcatcaacc tcaaaataaa gttccatatt atttgatttt tgtataattg
      301 tatctggatt aatccctgta agccatgcga agtcttcttg tatagcaaat cctcctttaa
      361 atgttacatt tttgcctaag tccaattcaa tttcttgctc aaataattct ttataaaatt
      421 tttttgatac ttcaatatcc ttcactgcta ataatgaaac ctgatatttc ataaattttc
      481 ctcctaattt tttatttcta ttttttcagt atcttctatt aacatttttt tctctatctc
      541 ttcagtacct tttattaatc ctttattttt acttttttag catcttctat taacactttt
      601 gtttctacct attcagtatc ttatatcaat cttttatttt ttcaatatct tctattaaca
      661 cttttatctc tatctattca gtactttcta tcaatctttt gtttttattt tttcaatatc
      721 ttctattaat acttttgttt ctacctcttc agtatcttat atcaatcttt tattttttca
      781 atatcttctg ttaacacttt tatctctatc tctttaatat cttatattaa tcctttattt
      841 ttattttttc aatatcatca ttaattccta tttattttat catcttaaat acgaaaacac
      901 tgtgtcatat ttgcaaaata tatattatca tctatattaa agcttcacaa tttatattta
      961 aaaattctct ttaattacac tacctctatt taaaaagcta ccttaattat acttataagt
     1021 aaaggtagct ttttaaattt aattaaatag aatatccact attaatttat atttaattta
     1081 tttagattca cattatagta tcataatcca cttataatca tttgtcatat tacttaagta
     1141 gactaatata ttacataaat acacttacat acactttata ttgatgctat aagaaatacg
     1201 tttgcttata cgtctttctc ctcttctttt agatttttta tccactcttc aataagtaaa
     1261 atatcatctt tgctcaaatt aatattttca tgtaatgaca acaataaact ttttatagaa
     1321 gaatcatgaa tatttctaaa aaaatctctt gtctcaactc ctatgtattc cttttcttta
     1381 attaaaactt cataatgcgt atatttatct atcttttgag aatttagaaa tctttttact
     1441 actaatctag ataaaagagt caaagttgtc gtttgtttcc attgatattt ttgctccatt
     1501 cctaaaacaa tatctctaga tgttactttg gaatctgatt tccatataaa tttcattatc
     1561 tttaattctg attgtggcaa cttttttata gtcatgataa aaccttgcta ctcagaatat
     1621 agacaagcaa tatccataat aaaatttttc gattaaatac atcttatacg gatttatata
     1681 agctcagact tataagatat ttttcaatct taatcctaaa tttagctatt tgtacacggt
     1741 aaatatatat aaataatgtc aaaattctct tgtattatat tgcttaattt tatgaaatat
     1801 tttgtactca gctatctatt ttggatactt gtccagtagc tctcctttct tttaaactct
     1861 ttgattttaa atatttgtat ttcagtaaat cctacttcct aaatttttta tttactacaa
     1921 cttattactg aacttcctac ttcctaaact ttttatttac tacaacttat tactgaactt
     1981 ctcacttact aaatttctta cttactataa attataacta aacttctcac ttactaaatt
     2041 tcttacttac tataaattac tactaaactt cctactttct aaatttctta cttgctacaa
     2101 attattacta aacttcctct ccctaaattt cttacttgct ataaattatt actaaacttc
     2161 ctactttcta aatttcttac ttactataaa ttattactaa acttcctact ccctaatttt
     2221 cttacttact ataaattatt actaaatttc c
//
LOCUS       DAYATN010000248         2238 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.252, whole genome shotgun sequence.
ACCESSION   DAYATN010000248 DAYATN010000000
VERSION     DAYATN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.252"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(81..1373)
                     /gene="brnQ"
                     /locus_tag="ACGI8A_003682"
     CDS             complement(81..1373)
                     /gene="brnQ"
                     /locus_tag="ACGI8A_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFH0181642.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          742 a          372 c          246 g          878 t
ORIGIN      
        1 ttaataattc ttttaaatat aaaaaggaat tgcctatata atttaaaggc aattccataa
       61 ttatcaatta gtttttatta ttatgcagtt ttactgcttt tagatggaat aaagtttcct
      121 attattcctg ctattacaac tggaactatc cagttaaatc ctaatgaagc aaatggtaag
      181 ctattaacta gaggaatttt tacaccaaaa cttgttgcaa ctgttaatac acttactaat
      241 aatgccatgt atgcagcaca tttgaacacg ttgtcatttt taattttatt actaaataat
      301 gctagtataa ctagagttat agttgctgga tataccatac ttagtatagg agaagagaat
      361 tgtattattg tacttactcc aaagtttgat atgattgcac taaatacaca aactactata
      421 actatatgct catactttaa ttttccatct gtaagtgttg agaaatattg accagctgct
      481 gaagaaagac ctatagctgt tgttaaacat gctaatgcaa ctataatagc caataaaatt
      541 ttacctgtag ttccaagtaa agctgctgtt attgcaacta ttaaagctgt ttgttgaaca
      601 tcttgtccat attgttgaga cacagttgct ccaagataac ataatccacc ataaactaat
      661 gccattccta ttactgcaat tacacctgca cttattgtgg ctttcatctt ttctttctca
      721 cctgtataac ctttagctat tactgacatg attataattg atgcaaatac actagctgcc
      781 aatgcatcca ttgtttgata accttgagtt ataccttctg caaatacatt ttgtattaaa
      841 gaactgtctc ttatttctcc taatggagat acgatacctt taactattaa tactgctaaa
      901 gctataagta aagctggagt taatattgac ccaactatat ctacaacttt tgatggtctt
      961 attgttaaaa ccaatactag agcaaagaat actatagaga atattactgg gcttattcca
     1021 gaacctattg taggtaatat acccatttca taagttgttg ctgctgttct aggtattgct
     1081 aaaagtggcc ctatacagat taccattgca catcctaatg caatacttaa tcctttgcct
     1141 gcccttgaga acatcttcga tgtgtctcca ttgaacttag ttgctgctat aactgctaat
     1201 aatgccaaac caccatctgc aaaaaggaat cctatgaaag ctactagcca actacttcca
     1261 gataaaactc ctagatatgg agggaagatt aagttccctg caccaaaaaa cattgcaaat
     1321 agtgcaaatc caattacaat tacatcttta aaattgattt gtttctcacc catttaaagc
     1381 acccccattt gaattttact accttttact aaaaaataag tccaatctct taattaaatg
     1441 aaaacctttt gacgagattt tatttaatta tacatttttg acttaaattt gtcaattatg
     1501 tttcataatt tataaaatct tcacaatatt taatacaact ttcttaatta aacaaaatca
     1561 acctgtatac tttaaacata gttggttttt tgttttttct ctaatttttc ttgataaaat
     1621 taactattcc gaataattaa aatattaaga ctttttagat tattcgattt attattaagt
     1681 tgtattgctt tatatactgt atatatcact ctcctttttt acattcatat aatcttttta
     1741 attcaattta aattttaaat attttgtgtt tattatataa tctgaaaaaa aagcagtcaa
     1801 tagcattttt aattttttta attttcaaaa ttatgattct taaaaaatcg ttttcctttt
     1861 atcataacac ctatttacgt taattcttgt agtattttgt atttattttg ctaaattgac
     1921 agttaacttt attcaatttt tattctactt gcgtagattt gcctcttgtt ttttactctt
     1981 aaaaccattt tttctgaaaa ttccttatta tgatttatca acttattttt cttcataaca
     2041 attactctct aacttccaac ttacttaaac tttgcagttg ctagtattca cgtttacata
     2101 cttatatgtc ataattaact aaaaattctt aatatatctc tcaatgaaaa caatttactc
     2161 atttatttaa tacatttata taatcatatg tcttaaattt tgttttatta ttttattatt
     2221 gtcacaattt tatttata
//
LOCUS       DAYATN010000249         2202 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.253, whole genome shotgun sequence.
ACCESSION   DAYATN010000249 DAYATN010000000
VERSION     DAYATN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.253"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(106..504)
                     /locus_tag="ACGI8A_003683"
     CDS             complement(106..504)
                     /locus_tag="ACGI8A_003683"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HFH0181643.1"
                     /translation="MIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETLQ
                     VHGRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPTPNTEFIFEAVQGEAIKRKGKM
                     LVKVIILDNKPVEVKIIGKAVIAFSTNIEI"
     gene            complement(564..983)
                     /locus_tag="ACGI8A_003684"
     CDS             complement(564..983)
                     /locus_tag="ACGI8A_003684"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HFH0181644.1"
                     /translation="MTKEKFTGNPAGVITNADGLSESQMQKIARELNNSETAFIFNNL
                     AKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAIENQFNNKTVFQKTGAGILPVD
                     IIKKDNDYEIIMTQGKIEVSPPLQQTYQKRLLNAFGI"
     gene            1321..1677
                     /locus_tag="ACGI8A_003685"
     CDS             1321..1677
                     /locus_tag="ACGI8A_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0181645.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            complement(1792..1968)
                     /locus_tag="ACGI8A_003686"
     CDS             complement(1792..1968)
                     /locus_tag="ACGI8A_003686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181646.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     regulatory      complement(2021..2110)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          780 a          296 c          288 g          838 t
ORIGIN      
        1 tatatattaa caaataataa aaaaattaat ttgattgtat ataaattagt ttgttattaa
       61 attagtatta aaaagttcaa aaatttactt agaaggaatc tataattaga tttctatgtt
      121 tgttgagaat gcaattacag cttttccaat tatttttact tcaactggct tattatctaa
      181 aataattact tttactaaca tcttgccttt gcgttttatt gcttctcctt gaactgcttc
      241 aaatataaac tctgtgttgg gtgtaggtat tactttataa tgtacaaggt acgcacctaa
      301 gggtccatta gcatttcctg taacagggtc ttctgaaata ccaattgcag gagcaaacat
      361 tcttccatga acttgtagag tttctttatt atgtaatgta aaaatataat atccattaca
      421 ttttatttct tgacttattt tcgataattc atataaattt ggagataaac tatttaatgt
      481 tgataattcc tttattccaa tcataacttt tgaatgtcct gtagatgata tggcaatagg
      541 acaatctgta cgaatatctt cctttaaata ccaaatgcat taagtaatct tttttgatag
      601 gtttgttgta atggtggaga tacttcaatt tttccttgtg tcataattat ttcataatca
      661 ttatcttttt ttataatatc aactggtaat attccagcac cagttttttg aaatactgtt
      721 ttgttattga attggttttc tattgctcta acataatggg cagcaatagt agcatgacca
      781 catataggaa cctcattagt tggtgtaaaa aatctgatat gtacatcata attttcttga
      841 tgatttttag ccaagttatt gaaaataaaa gcagtttctg aattatttaa ttctcttgct
      901 attttttgca tttgtgattc agataagcca tcagcatttg taattacacc agctggatta
      961 cctgtaaatt tttcttttgt aatgagtcaa cttgataaag acgaaatttt ttattcatat
     1021 tatataatcc tttctaaaca ttagattagt ttatctacat agaaaacttg agataaatta
     1081 ttatttatta ttatctgttt aaataaaatt aataaaataa catatgtcta tattaaaatt
     1141 taaacatata catctaaaaa agtagtatta tgtaaggctc tatgctacac tgaacctccc
     1201 atgactaaag tcacgagtgt gcatagctat tttttaatca agattatatt acgaattagt
     1261 acgctaaaca agaatgcact ttttagttag atacttacta ttttgaaaga gaggattgag
     1321 atggatatag ataataattt tgaagaattt ccaaatattg aagaaagaca aaaagatttt
     1381 aattgttcaa ttggttttac catgactgtg ataggaagta aatggagggc aattatttta
     1441 tggcatattt taaaacaaga gccaatacgt tacagtcaat taaaaaaaga agttgttcat
     1501 ataagtcata agatactgtc acaagaatta aagtatcttg aaagagatgg acttattaag
     1561 aggatttctt atccgactat tcctccaaaa gttgaatatt tgtctactaa acgtggtaaa
     1621 tcattggaat ctatattatt agaattatgt aattggggta aacagtatat gagataaact
     1681 taactgttta cttcatgaaa aatatataat gaagcttatt ttaattagtt ctttaatgaa
     1741 ttataaaacg tagataaaac taaaaagggt tttctagaaa ataaaatttc tttaattata
     1801 tctgcttacc aataccatca cttgatgcaa acaagattct accacattct ctccatattc
     1861 tagtataaaa tcatttaatt gtgtatctat aaaattgcag taatcgcgtg aactgcatat
     1921 atatgcagtt agtatatgtg tacatctatc cattttatcc ataaacatat atcatatatc
     1981 cccctaaaaa acattcataa aaacaaaaag aaaatatctc tttggcaact ggctgacata
     2041 actcatttca taagtcttag tccctatagc tttgcgtcac taaatttctc tagttttgcc
     2101 tatttagttt tattctacta ttaccataat ataggaaaac gtttcttttt tcaaggtgca
     2161 tattaaaaaa tttaaaaatt aaaaaaatat tttatttctc aa
//
LOCUS       DAYATN010000250         2142 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.254, whole genome shotgun sequence.
ACCESSION   DAYATN010000250 DAYATN010000000
VERSION     DAYATN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.254"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            117..515
                     /locus_tag="ACGI8A_003687"
     CDS             117..515
                     /locus_tag="ACGI8A_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181647.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    951..1214
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1365..1916
                     /locus_tag="ACGI8A_003688"
     CDS             1365..1916
                     /locus_tag="ACGI8A_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HFH0181648.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          908 a          198 c          307 g          729 t
ORIGIN      
        1 ataataaaaa taatattaaa gataataaga tatataagaa acttgaaaaa atttgtaatt
       61 tttagttata tatatattat aatgaactag ttaaaaattt ttatgagggg gaaaatatga
      121 ataagaagaa aaaattgatt gggatagcta tatcttttct acttataatc agcattataa
      181 atccattaca agtagaagca gaagctatta ttgagtctag tgtatacatt tcttcaataa
      241 aagagaatta tacatatgat aatactaaat ttaaaactat taaatctaaa agtagacata
      301 aaagtaggaa atataataaa agtaaagtaa agttgaaaaa acgaagtata agaactaaaa
      361 agtatggata tcaaactata aataaatata aaagacaaaa cgcaagaaga cataatatcc
      421 taacaaaatt aatattgtcc ataatagtca tatttttggt tatagtttta gtagtgctaa
      481 tatttattaa aagaaagaag aaaaaatttt actaaaaata attaaaagaa tatcattttt
      541 ttaatcattg gtatctattt aataatatga tgtaattaaa atggagtaat attctgtttc
      601 aaaggaggca tataaataca ggaatgagta ttaaatgatg gttaattaag taaatttatt
      661 gataaatgta cttaattaac tttgaagaat ttaattcctc atgaatcaaa atataaaatt
      721 acatcaacag ttattcacaa aattaatata agaagaatta gagccaaaga gcagactatt
      781 agaccattaa agtaattatt catagaattt tgtatatgaa aagaaatgta tagtataatt
      841 tacttttttt ataaaaaata gattacaaaa tatatttttt ataaaaaggt attgacattt
      901 tttagaaaaa agaatatcat tattttaaca acaaaattca atacaataaa ttaaatccta
      961 tgacaaggaa gagtagttaa atttaggttc agtatagaga gctgaggatg gtggaatctt
     1021 agcaagaaaa aatttgatga atggccctta gaggaataaa ttgaaatcat agttttgaga
     1081 gtagacttta tcgtcagtcg cacgttatag cgaataaggt atgtaagtac ctgataatga
     1141 gatgtatgaa aattgaattt tatattaatt tttatataat ttaggtggca acgcggataa
     1201 tctccgtcct attagtttag gacggagttt tttattttac tgaaaattta aaagatagaa
     1261 ggtggtgaga aagatgttat gaatttcaaa agctaaagat gatgatatag taggttggca
     1321 aaaaaataaa ttgaaaatta aaaaaatagg aggaagtaca aaaaatgaaa acaaatacaa
     1381 aaaaattaac tttaaatgca atacttttag cgatgggatt attaatacat caaatcacac
     1441 cagctatagg attaccaatg aagccagatg taccactagc aatgttattt gtaatattgg
     1501 ttttaaatag agatgattat aaaacttgtt tgatagcggg tatagtaaca ggaatattta
     1561 cagcacttac atcaagtttt ccgggaggtc aaattcctaa tgtaatagat aaaactctta
     1621 cagcaaatat agtattttta ttaatgaata ttagttataa tatgcctttt ataaaaaaat
     1681 tagctaagaa aacacaagat actgtagtag ttgcaataat aatgccaata ggaactcttg
     1741 taagtggaac tatcttttta ttagcagctc aagctatagt tggtcttcca ggggcttcat
     1801 ttacagcttt attcttagca gtagtattac cagctgtttt aataaatcta gttgcaggaa
     1861 tatttttatt caagattgtt agtctatcaa taagaagagt tagttatcaa gcttaaacac
     1921 gaaataatat tttactttat aaagcattat aataactaaa taacataaga ataaagtaga
     1981 ttataactat tttattctta tgttgtttaa agatttggta ttatatattt atttttaata
     2041 aaatatttat atagtaccaa ttacacttaa tttaacagat aaattatcat acctatataa
     2101 tattcttata tgtatctcat ttgtagttat taaatataag at
//
LOCUS       DAYATN010000251         2125 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.255, whole genome shotgun sequence.
ACCESSION   DAYATN010000251 DAYATN010000000
VERSION     DAYATN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.255"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            43..2067
                     /locus_tag="ACGI8A_003689"
     CDS             43..2067
                     /locus_tag="ACGI8A_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0181649.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT          904 a          243 c          300 g          678 t
ORIGIN      
        1 aaatacttta aaaataaata ctattttgtt aggagaaata aaatggataa aaagaagtta
       61 atccttataa catatgataa attgaatagt gaccactaca aagaagaatt aacaaacttt
      121 tttggagacg aaataatcat agaaacacaa aatatacttg atggaataaa agaaaattta
      181 gagggagatg tagtgctttc tctatcacct ttaactagta attttttaat aaaacatttt
      241 aaggaagata ttgaaattat tcatggaacg aaagcacttt caaaacttgg atatgaaaaa
      301 atgatgaaac tgcctccagg tacaaaatcg ttacttatga caaccaataa aacttcagca
      361 tttgagatgg ctacttatct atataaaatt ggtataaatc atatagattt tgtaccgaca
      421 tatccagatt gtgatgaaat atatgattta gatacagcta taacaccagg tcagattaga
      481 tttataccaa aatacataaa aaatattgtt gatttaggtt ggagaaaaat ctcattagat
      541 acatatatgt cattattagt agtattaaaa ctaaaaaatg agaaatttat agaaaaatta
      601 tacaaattgt ccaaggagac actaagtcat gatttcttaa atacatcatt agataatata
      661 tctaaattaa aaactatact ttacatgacc attgatgaaa taggtgatgg tctaatattt
      721 ttcaatacct ttaataaggt tacttttgta aataagtctt tacttaatat gttggaacta
      781 gatgaaaaac ttataaaatc accttcctta atggaatata tgccaaagag tttcttagat
      841 aaaattacaa agaacttaaa tatagataat atgattatat acatagacga aatagataaa
      901 aaattcatac tttctaaaaa accattttat ctttataaaa atatagaggg ttgcttaatt
      961 acattaaaag atgtaaacaa tatagaaata ttagaacaaa aaataagaag tgattctgtc
     1021 aaaagaggtt atgtagctaa atataaattt aataatatta ttggaaatag ttctattata
     1081 aaagattgta ttaaaaaggc aaaaaaaatg gctcttacag ataatcctat attaataaca
     1141 ggtgaaacag gaacaggtaa agaagccttt actcagtcaa tacacaatca ttcaaataga
     1201 aaaaataaac cttttgtagc aataaattgt gcttcattac caagtgaatt acttgaaagt
     1261 gaactatttg gatatgaaga tggttctttt actggagcaa aaaaaggtgg aaaaaaagga
     1321 ttattcgaat tagctcatac tggcactata tttttagatg agataggaga tatgccacat
     1381 gatttacagg ttaaactttt aagagtctta caagaaaaag aaattagaaa aattggtggt
     1441 acaagtatta ttccaattga tgtgagaata ttagctgcca caaataaaga tttagagaaa
     1501 ttaattatag aaaataaatt taggatggat ttattttata ggataagtat gttcacttta
     1561 gatttacctc ctcttagaaa gagattagaa gatatacctc ttcttttaga atcattttta
     1621 aaagaacttc cctataaaaa cataaaactc gataagagtc ttttagaagc tttaaattca
     1681 tacacttgga tggggaatat tagagaactg agaaattgtg ttgaatatat ggcttacatg
     1741 ggttccaatt atcttacaat aaatgattta cctcaaaaca tatcttcaaa attaaataat
     1801 aatcatatgt caagtaatat gtccatattc aatgatttaa accaatatga caaaaacata
     1861 tgcatttcta tcctaaaatc attgcacatg aaaccaatgg ggaggacaaa gttaatgaaa
     1921 tttatggaat ataatgtcac agaatacgaa gtaagaaata tgctagaata tttgacacgc
     1981 aatggatact taatttctag taagggaaga aaaggctcat caataactga gaaaggcata
     2041 aaaataatag aaaataatat aatttaaacc tatagtaatt catctttact ttatcaaaat
     2101 ttatatataa ttataatttt ttatt
//
LOCUS       DAYATN010000252         2098 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.256, whole genome shotgun sequence.
ACCESSION   DAYATN010000252 DAYATN010000000
VERSION     DAYATN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.256"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            96..710
                     /locus_tag="ACGI8A_003690"
     CDS             96..710
                     /locus_tag="ACGI8A_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFH0181650.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            728..1090
                     /locus_tag="ACGI8A_003691"
     CDS             728..1090
                     /locus_tag="ACGI8A_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0181651.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1196..1693
                     /locus_tag="ACGI8A_003692"
     CDS             1196..1693
                     /locus_tag="ACGI8A_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFH0181652.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          812 a          233 c          331 g          722 t
ORIGIN      
        1 tttttgacaa gtctttattt tgtctaaatt aaacaaactt ttttacaaat aaatacataa
       61 aatttaaact aatctattta ttggaggaga taaaattgga agaagtaatt gcaaaaatta
      121 ttactataga aaatggattc aaagaaattg aaaacattgc taaaaaaatt gtaaaaaata
      181 atgactcaaa aaagtgttat tatctatcaa tctacttata taggtcagaa tattatcaag
      241 taagagaatt ggctgtgttt atacttggtt ttatatcagt aactatttct gaagctttgt
      301 tatttttaaa agataatgtg agtaaagatg aaaactggag agtacaagaa atactagcta
      361 aatcatttga ttattattgc agtgaagtag gatatgaaaa ggctcttcct gttataaaag
      421 aatggcttaa ttctgatagt ccaaacataa aaagggctgt gacagaaggt cttcgaattt
      481 ggactagtag agactatttt aaatctaatc ctgacgttgc tatatcacta ctttctagtt
      541 taaaagaaga tgatagtgaa tacttgagaa agtctgtagg taatgctctt agagatataa
      601 gtaaaaaata tccagattta gtaaaaaaag aattagattc atgggatata tcaaataaaa
      661 aagttgagtt tgtatataag cttgcttcaa aatgtatctt ggcaaagtaa aatggaggtg
      721 aacttatttg aaacttcgtg atgagtatac ttgcccatta gaactaacac atgatatgat
      781 aaaaggcaaa tggaaatcta ttatattatg gagattacgc ataggaccaa cttccttatc
      841 aaaacttgaa aaggaaatca atggtattaa tcaaaagatg ttacttgaac aacttaaaga
      901 attaattgaa tttggtcttg tagataaaaa gacatatgaa ggttacccac ttaaagttga
      961 atactcactt acacctattc gagggaaaca aatattggat gctttggcta taatgcaaga
     1021 tataggaatt agctttatgt tggataatgg tatggaagat attcttaagg aaaaaggtgt
     1081 tttaaaataa atagataccc acaaaaaagt aagtaattta ccattatttt caactggtat
     1141 ataattaaat tatagatata ttctattaat ttattataat aggaggtaaa ttattatgaa
     1201 agtaactagt acaggtatag taaatggtgt tatagaagat aaatacggta aaagaggaac
     1261 tcagttcaat gaaggtggta tgcctacata ttcactacct ctaaagtttg aagatgctcc
     1321 aaagaataca gtatcctttg ctgtattttt agaagataaa gactctgttc ctgtatgtgg
     1381 gtttgcatgg atacattggt tagctgtaaa tataacaaaa gatgagttaa atgaaaatga
     1441 aagtgtaaca gctactgatt ttgtacaagg gactactagt tggcatggca aactaggagg
     1501 tcttgacaga ttagaggcca gtgtatatgg tggaatggct cctccagaca gacctcatgt
     1561 ctatgaaata catgtatttg cacttgatac tatccttgac cttgaaaaag gattctatat
     1621 gaatgaattt ttcaaagcta ttgaagggca tgttttagat acatttacac taaaaggaag
     1681 ttattctaat taattaggtt gactatagct aatcattaat taaatgctac atttaattga
     1741 tgattagctt aattgttatt agaatatttt ctgcacttta tcttaatata tattttagtt
     1801 gactggtatt ttaatttcta ttagattatt atcaatttat ataaattgat tttagcttaa
     1861 aacttactta tctatttcag tttctattag aattattatt agactcatta aaataaaagc
     1921 actaatataa ctaataatat atgttaaatt cattaactct acataaagtt ttataatcta
     1981 aatgaaatac aataatctgc ataaaattca atagcttata taaaatttaa taatttatat
     2041 aaacattatc aatctatata aaatttagta atttatataa atattatcaa cctatata
//
LOCUS       DAYATN010000253         2050 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.257, whole genome shotgun sequence.
ACCESSION   DAYATN010000253 DAYATN010000000
VERSION     DAYATN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.257"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            209..1345
                     /locus_tag="ACGI8A_003693"
     CDS             209..1345
                     /locus_tag="ACGI8A_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFH0181653.1"
                     /translation="MIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLIN
                     EIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYMI
                     YNNHRALEYGLDNLDELYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEIIH
                     TGLEPLKIYDFISKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYLY
                     LIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEMR
                     NMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLKA
                     GIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1371..1559
                     /locus_tag="ACGI8A_003694"
     CDS             1371..1559
                     /locus_tag="ACGI8A_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181654.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          874 a          164 c          287 g          725 t
ORIGIN      
        1 aatatataac taatgtatta taaataatag ttgtaaactt cctgtaacat atattttaaa
       61 tgcttattct taaatttttg cattatttat taaaatttat tatgctttaa cgctaaattg
      121 agtttgataa ttataaaaca aataatcaaa aatagtgtaa aaattaatac tttttgttaa
      181 tattaataga taaagataat ctaaggtggt gattaaactg acaatagaag aatacaaaaa
      241 caaatacttt actggaaata aaatcgtact ttctataaat agttttgata ataaagaact
      301 atactatcat gaaactgaaa atatattaaa tttaataaat gaaatagata aattaacatc
      361 aagtaagata tttaaaacaa tagaagataa aaatgatttg gcactagagt cttattattc
      421 tagtgtaata gaaggtgctt tttcaactag aaagattgca aaatctataa ttagaggaaa
      481 gatgaaacca agtaataaaa gtgaatatat gatttacaat aatcatagag ccttagaata
      541 tggattagat aacttagatg aattgtatag tcacaaattt atatatgatt tgcatcatat
      601 attgggcgaa aactgcttgg attcagaaga atatgagtat agaactgaaa aagtatatgt
      661 atgtgattca aaaggtgaga ttatacatac agggcttgaa cctttgaaga tatatgactt
      721 tattagtaaa ttaatagatt ttatggaaaa tagcaaagta agtaatttaa ttaaaagtgc
      781 tataattcat ttttactttg tatatgttca tccttttagt gatggtaatg gaagaacatc
      841 aagagcctta tcatatctat atttgattga taaaggatat gatactttca aggatttttc
      901 aatatcatat atgatatcta aaaacagaac taagtattat aaggctatac ttgatgtaga
      961 aaacaaagga aataatttaa ctgtatttat agaatttatg ctcaagtcta taatacaatc
     1021 tattaatgag atgcgtaata tgcatgatag aaaatcttta gaaagtatat taaaagaaga
     1081 attgtttgaa aatgacataa ctctatcagc aacagaagaa catatattaa aatatatttg
     1141 caataaagat aactattcta tgacattaga aaattatata aaaaagaata aaagtagata
     1201 cttaaaggct ggaataaagg aaattgaatt agttgaccag ttgatggaag tgtttaataa
     1261 tcttgaagag atagagattt tgtctaaaga aaaaaatata tataaagtta atgataagta
     1321 cttaaaaatg cttgatatag actaatttag ataatctgga gagggaatat atgaataata
     1381 agaaaaaggc atttttgatt tctggggcta taaacgtaat ttcattaatt attgtttttt
     1441 cattgtttca ttataatatt atagaaaata gtacacaaaa tatgataata actgtaatct
     1501 taatttttat ttttgaaatg ataaaaataa aaatcataga gaaatactat aaactataaa
     1561 caagaaatat cttttctaag agcatattgt acaatatgct cttaatttag ttatatagat
     1621 ttaatataaa taatgtattt tttgtagatg atttatgtaa gatttatgac caatttgtgt
     1681 atgatttgtg ttctttaatc atgacaatat tatattgtaa aaaataaatt taataaaaaa
     1741 tgacttggaa tttatattct aggtcttttt tatataaaaa tttaataagt ttcaaatgtt
     1801 ggtaactttg acagtagaaa acgtctattg agataacgag gattctaatt gaggtggaga
     1861 gatattagca cttaaagtag tatatgcaac agatataatc tttacaaaag taatttgaat
     1921 taaaatgtaa atataaagaa taattaaaaa aatatcgcaa ttatttgttt ttcatatgga
     1981 taaaattggt tatttaatat ataattacaa gtataaataa taaaatttat ttgtaaaaag
     2041 agtagcactt
//
LOCUS       DAYATN010000254         1967 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.258, whole genome shotgun sequence.
ACCESSION   DAYATN010000254 DAYATN010000000
VERSION     DAYATN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.258"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(39..1256)
                     /locus_tag="ACGI8A_003695"
     CDS             complement(39..1256)
                     /locus_tag="ACGI8A_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HFH0181655.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(1447..1860)
                     /locus_tag="ACGI8A_003696"
     CDS             complement(1447..1860)
                     /locus_tag="ACGI8A_003696"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0181656.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          669 a          324 c          225 g          749 t
ORIGIN      
        1 ttagattata tattttaata tactttctta tataagaatt attttaaatc aagattatct
       61 agtattttta aaaatatttt tacagcagta tccaagtcat ctacagagac actttcatta
      121 ggattatgac ttacgccccc agcgcatctt ataaatacca taccaatatc agttatatta
      181 tccatttcct gagcatcatg gccagcacca ctataaatat aaaaaggatt aagatttaaa
      241 tctataaaac ttttttctat gatttttgtt attttattag aacatggtac aggaacattt
      301 tcaaaagcaa gttcacttgt ataattaagt tttctatttt cacatatatg tgatatttca
      361 ttaaatatct tttctacaga attatcaaga atttcttgtg aacaagagcg tacatcgagt
      421 gtaaattcag ctctttcagg taccacattt gatgaagatg gatagaaatt catctttcct
      481 acagtagcaa ccaaatcagt agtcgtttta gctacttttt cgatagctaa tacacattca
      541 caggcacaac atccagcatc aagtctcata ttcattggaa gtgtaccagc atgtccagac
      601 ttaccattta ctgatatctt aaaactttta aatcctgtaa tacttgatac aattccagca
      661 ggaagagatt cattttctaa aacaggaccc tgctcaatat gaaattctaa atatgcatct
      721 acatcttttc taggatgcaa atttgttaag ttttttgtat taaatccaaa ctttgttaca
      781 gcttcttcaa gtgtaattcc attttcatca acagatttaa gaagttcctc tttaaaagta
      841 ccagcaaaaa ctttacttcc cattaaagaa gtttcaaatc tagttccttc ctcttcagcg
      901 aaagcaatta gcttaatatt gaaaggataa gacctattat tgtgttttgc ttcttcaaga
      961 ccaactagag gcactattat acctaacata ccatcaaata tacctccaca ctttacacta
     1021 tcttgatgag atgcaactac taatgtggga gcatttggaa agcttgattt atatgtacct
     1081 ataacgtttc caatgtcatc aatttcaata tcaagattta aaccttccat ccatgaacta
     1141 attaaatcaa tagatttttt atgttcttga gtcaagataa gtcttgtaag accatttgtt
     1201 gttgaactta tttttcctaa tgtttgtaga taatccatac atttttttcc taacatattt
     1261 acctcctaaa aatataattt ttatttaagc taagattagc ttttatactt ataatttttg
     1321 aactttcaaa taattttgta tgtatagtaa aatactattt tagtattaat aatgatataa
     1381 aaacataaag tctgtagacg aagagcagaa gttttaattt aaaaatatga aatatgattt
     1441 ttattattat agtatttttc tataataata tctctttcca gttagaggat aattttcaag
     1501 tgaaaatact ttatggtatc caaatttctc ataaaattta ggtgcttgaa aactgaatgt
     1561 atctaaaaaa acatatttac aacctcgttc tttagcaatt ttttcagcct tatataaaag
     1621 ttgacttcca gtacctttat atctaagctt ctcactaacc cataagtatt ttatagaaag
     1681 ccaattacca tgagtaacac ctataaaacc tgcaacaatt ttacctgctt tatctcgaat
     1741 atatattcct aaatccactg ggtttttatc ttcaagtcta gcaagattat attttaataa
     1801 tccttgaaaa atagtatctt catcttgttt ttctattttt tctgtaattt ctaattccat
     1861 atttgaactt tctccttgta taatcttttg tatattggta ttttttaata aaatcagtag
     1921 aaagaatata gtttaacatt attctaatag gcatttatgc taatgtc
//
LOCUS       DAYATN010000255         1951 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.259, whole genome shotgun sequence.
ACCESSION   DAYATN010000255 DAYATN010000000
VERSION     DAYATN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.259"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..255
                     /locus_tag="ACGI8A_003697"
     CDS             <1..255
                     /locus_tag="ACGI8A_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HFH0181657.1"
                     /translation="SIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLF
                     NATLEGEEPKIDTITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            267..1166
                     /locus_tag="ACGI8A_003698"
     CDS             267..1166
                     /locus_tag="ACGI8A_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFH0181658.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            1166..1399
                     /locus_tag="ACGI8A_003699"
     CDS             1166..1399
                     /locus_tag="ACGI8A_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181659.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            1409..1810
                     /locus_tag="ACGI8A_003700"
     CDS             1409..1810
                     /locus_tag="ACGI8A_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181660.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIDFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTTYGKNKILTFRCLRW"
BASE COUNT          826 a          229 c          359 g          537 t
ORIGIN      
        1 tctattaaat gtagggatga aatagcgtta aaagctcatt tagatataga agcactaaga
       61 aatagtaaag atagacaaaa agcttttgaa gagcaaataa accctttgaa acaggataaa
      121 gattatttgt ttaatgcaac actagaaggt gaagagccta aaatagatac tataacacca
      181 gggcaagagc ctaagataaa tgattttggt tttaatttta ctggggtaag acctcatgaa
      241 aataataata aataggagga aataaaatgg cagcactaaa ttatgcaaaa gaatattcaa
      301 atgttttagc acaagcatat ccttatactt taaacttcgg ggatttgtat gcaacaccaa
      361 ataatggaag atatagatgg actggttcta aaacaataga aataccaact atatctacaa
      421 ctggaagagt agattcaaac agagatacaa tagcagtagc tcaaagaaac tatgataatg
      481 cttgggaacc taaggtatta actaatcaaa ggaaatggtc aacattggtt catccagcag
      541 atataaacca aactaattat gtggcttcaa taggcaatat aacaaaagta tataatgagg
      601 aacaaaagtt tccagagatg gatgcttact gtatatctaa aatatatgct gattggaccg
      661 cattaggtaa cacagcagat acaactgttc ttacaacaac aaacgtatta gaagtatttg
      721 ataagttaat ggaaaaaatg acagaagcta gagtacctga aaatggaaga atattgtatg
      781 ttactccagt agtaaataca cttatcaaaa atgcaaaaga gatacaaaga acagtaaata
      841 taaaggatgc aggaacttct cttaatcgtc aaacaactga tattgacaca gttaaaataa
      901 ttaaagtacc atctaatcta atgaaaactg catatgattt tacaactgga tggaaagtag
      961 gagcaggagc taaacaaatc tttatgtcct tagttcaccc aagtgcaata attacacctg
     1021 tttcttatca gttctctaag ttagacgaac caacagcagt tacagaggga aaatacttct
     1081 actttgaaga aagttttgag gatgtattta tattaaataa aaaagctgat gcaatacaat
     1141 ttgttgttga aggagctgga gcataatggc acaagtaagg aaattaaata gaatattaac
     1201 tatagaagaa tgtaaaatag atgatttctt agagatggga tatgatttga tagatgaaac
     1261 tggtaaggca gtaaagtatg gcaagtcatt aaatgtaaaa gatttaatag ctgaaaataa
     1321 tattttaagg tcaaaagttg agtctttaga agaagaaaat aaacagctta aagagaaaaa
     1381 taaacttact aaaaagtagg tgaaaattat gggaaataat ataattgatg atatagaaaa
     1441 aagacttgaa agttttggat atatattaaa agatggggat aagtggttaa tagattttgt
     1501 aagagaaaaa atagaaaata ttattaaact agattgtaat ataaaaacta tgccaattga
     1561 attgaaagaa attgaagttg atatgatagt tggagagttc ttatttacca agaaaaatat
     1621 ggggcaatta gatatagaaa gcattaactt tgaagctgta gaaaagtcta tatcagaagg
     1681 tgatacaaag gtagattttg ctataggaag tggctctcaa acaccagaac aacgctttga
     1741 tagcttagta gcttatctta ctacttatgg taagaataag atattaacct ttaggtgctt
     1801 aagatggtaa gtaaaactag aaaagcaata gaaatgttat atagagataa atgtactata
     1861 gttgagtatc agccaattaa agaccctgta acaaaacgaa ctaacaataa agaagtgatt
     1921 gtattagaaa atcaaccatg taaactttca t
//
LOCUS       DAYATN010000256         1942 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.260, whole genome shotgun sequence.
ACCESSION   DAYATN010000256 DAYATN010000000
VERSION     DAYATN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.260"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            158..1735
                     /locus_tag="ACGI8A_003701"
     CDS             158..1735
                     /locus_tag="ACGI8A_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HFH0181661.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT          801 a          185 c          368 g          588 t
ORIGIN      
        1 tttatcattt aaataatgaa aaataggatt gtctatattt ttagatagtt ttcttattat
       61 tgatgaaaaa tgtaataatt ttaaactgta aaatagtgta aagttatttt ataatataaa
      121 tatagtaaga ttttaaacaa aataaggggg aataataatg aaagcaccaa aaactatttt
      181 aacaatactg acaatagcgc ttactttaag tagcatttct ataataccat catatgcact
      241 tacagaggaa aaattaatag gtaatggtag atatgaaact gctgtaaaga taagtcaaaa
      301 ggcatacagt tctagtccta atgtagtgct agttaatgac aattcattag ctgatgcact
      361 ttcagctaca ccatttgcaa aggctaaagg agcaccaata cttttgacag aaagtgataa
      421 gctagatgat aggactgaaa aagagataaa acgtctagga gctaaagata tttatttgat
      481 aggaggtact gctgtactta ataaagatat agagaacaag ttaaaaagta atggacttaa
      541 tgtagaaaga attaatggta aggatagata tgaaacttca ctgattttgg ctaataagct
      601 taaggatata aaagatatta aggaagttgc tgtagtaaat ggtgaaaaag gattgagtga
      661 tgctgttagc gttggggcac cagcagctca aaataagatg cctataattt tgtctagtcc
      721 taaggacgga gtagaagcat ttgataaatt tataagggat gagaaagtta taaaagcata
      781 tgtaattgga ggtacaaatt ctgtttcaag agctgtagaa aagagtcttc ctaatgcaga
      841 aagagtgagt gggaaagata gaaatgagac taatgctaag gtcatagaaa agttttacac
      901 tgatacaaat ttaagtaatt tatatgtaac taaggatggt agtaaaaatg agaatcaact
      961 aatagactcg ttagctgttg gagtattggc tgcaaaaaat gagtctcctg ttgttttggt
     1021 tggtaataaa ttaaacacaa aacaaaggga catacttagt actaaaaaat taaatactat
     1081 aacacaggta ggtggaaatg gaaatgaaga agcttttgat gaaattaaaa gtctacaaga
     1141 aaaaactgtg tttgaagcaa aaactgttga ggaattaact gatatgataa atatagcaag
     1201 tcctaatgat attataaact ttaaaccaaa agagaatact gtaaatgaag cttttagaat
     1261 ggtaacaaat aaaccaataa ctgtgaatat aaagggtgat tgttcaaaga ctcttacagt
     1321 agatatgcct aatggtgaag tgaataacta tgctactttg gtaaatgtta tagttagaaa
     1381 tattggagaa ggtggattta ataaccatga tacaatcact atattgtcag ttcgtgacaa
     1441 aaatggtaga gttatagaaa atactagaaa ttcagatata gatacgttga tgatattagc
     1501 ttctgctaat gatacaaaat tgattaatga tggatatata ggaaaactta tagataattc
     1561 atctaatagt gatataacta ataatggtac tatagataaa aaagttaatc aggttgaaga
     1621 tttagaggct aaagtagatt caattgaaaa agctatagat tctataagtc aaaaagtcaa
     1681 taaaatacaa gatatactag ataagcttgg atttctaaag aaatttctta gctaagaata
     1741 tatatattta agtgaagtgt ttgttgtaat tgttctaaag ttatgtagtt aaacaggttt
     1801 atatacaaat agatttatag ataaatatgt agtttagtat atatttgaat aagctttggg
     1861 gaggtaacat ggggattgga attgaatgta acaaaaaaat aaataaaagt attgacaaaa
     1921 ctagaataaa gaaaaataat ct
//
LOCUS       DAYATN010000257         1926 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.261, whole genome shotgun sequence.
ACCESSION   DAYATN010000257 DAYATN010000000
VERSION     DAYATN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.261"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="ACGI8A_003702"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="ACGI8A_003702"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            242..316
                     /locus_tag="ACGI8A_003703"
     tRNA            242..316
                     /locus_tag="ACGI8A_003703"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Asn,seq:gtt)
     gene            321..395
                     /locus_tag="ACGI8A_003704"
     tRNA            321..395
                     /locus_tag="ACGI8A_003704"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Glu,seq:ttc)
     gene            401..476
                     /locus_tag="ACGI8A_003705"
     tRNA            401..476
                     /locus_tag="ACGI8A_003705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:434..436,aa:Val,seq:tac)
     gene            482..558
                     /locus_tag="ACGI8A_003706"
     tRNA            482..558
                     /locus_tag="ACGI8A_003706"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:516..518,aa:Asp,seq:gtc)
     gene            569..643
                     /locus_tag="ACGI8A_003707"
     tRNA            569..643
                     /locus_tag="ACGI8A_003707"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:tgt)
     gene            658..742
                     /locus_tag="ACGI8A_003708"
     tRNA            658..742
                     /locus_tag="ACGI8A_003708"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Tyr,seq:gta)
     gene            752..825
                     /locus_tag="ACGI8A_003709"
     tRNA            752..825
                     /locus_tag="ACGI8A_003709"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Gly,seq:tcc)
     gene            836..912
                     /locus_tag="ACGI8A_003710"
     tRNA            836..912
                     /locus_tag="ACGI8A_003710"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Arg,seq:tct)
     gene            922..997
                     /locus_tag="ACGI8A_003711"
     tRNA            922..997
                     /locus_tag="ACGI8A_003711"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Gln,seq:ttg)
     gene            1009..1084
                     /locus_tag="ACGI8A_003712"
     tRNA            1009..1084
                     /locus_tag="ACGI8A_003712"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1042..1044,aa:Lys,seq:ttt)
     gene            1087..1175
                     /locus_tag="ACGI8A_003713"
     tRNA            1087..1175
                     /locus_tag="ACGI8A_003713"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Ser,seq:tga)
     gene            1193..1283
                     /locus_tag="ACGI8A_003714"
     tRNA            1193..1283
                     /locus_tag="ACGI8A_003714"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1227..1229,aa:Ser,seq:gct)
     gene            1292..1368
                     /locus_tag="ACGI8A_003715"
     tRNA            1292..1368
                     /locus_tag="ACGI8A_003715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Pro,seq:tgg)
     gene            1454..1529
                     /locus_tag="ACGI8A_003716"
     tRNA            1454..1529
                     /locus_tag="ACGI8A_003716"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1487..1489,aa:Trp,seq:cca)
     gene            1590..1666
                     /locus_tag="ACGI8A_003717"
     tRNA            1590..1666
                     /locus_tag="ACGI8A_003717"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Pro,seq:tgg)
     gene            1673..1749
                     /locus_tag="ACGI8A_003718"
     tRNA            1673..1749
                     /locus_tag="ACGI8A_003718"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1707..1709,aa:Ile,seq:gat)
     gene            1753..1828
                     /locus_tag="ACGI8A_003719"
     tRNA            1753..1828
                     /locus_tag="ACGI8A_003719"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Phe,seq:gaa)
     gene            1836..1912
                     /locus_tag="ACGI8A_003720"
     tRNA            1836..1912
                     /locus_tag="ACGI8A_003720"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1870..1872,aa:Met,seq:cat)
BASE COUNT          485 a          441 c          524 g          476 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      181 tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgtgaatg
      241 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      301 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      361 cggtaacagg ggttcaattc ccctacgagt caccatttgt gggatcatag ctcagctggg
      421 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      481 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtt gagggttcga
      541 gccccttcca ggtcgccaaa ataaaaatgc tggtgtggct caacggtaga gcagctgact
      601 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccatgtaaaa ctaatacgga
      661 ggatttcccg agcggccaaa gggggcagac tgtaaatctg ttgtcatcga cttcggtggt
      721 tcgaatccac catcctccac caattaaata tgcgggtgta gctcaatggt agagttctgg
      781 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaagaa agcgtgggtg
      841 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      901 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      961 catgtatgcg taggttcgag tcctgcatcc ccagccaaat aaaaatatgg tctattagct
     1021 cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc
     1081 accaatggag aggtgtccga gtggtttaag gagctggtct tgaaaaccag tgacttcgaa
     1141 aggggccgtg ggttcgaatc ccaccctctc cgccaaaaaa atgactgaat ttggagaagt
     1201 actcaagtgg ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg
     1261 ttcgaatccc tccttctccg ccattcaaaa acgaggtgta gcgcagtttg gtagcgcaca
     1321 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaac ttaagctttt
     1381 agttcagttg gttagagtgc ccaactcatg gctagtaatt ctagggtttg agtccatgaa
     1441 ggttattctt cataggggtg tagctcagtt ggtagagcgt cggtctccaa aaccgtgcgc
     1501 cgggggttcg agtccctcca cccctgccaa ataaaaagga cttgaaaaaa gttggatttt
     1561 agagtagtat aagatagtaa ataagatttc gaggtgtagc gcagtttggt agcgcacatg
     1621 gtttgggacc atggggccgg gggttcgagt cccttcacct cgaccaagaa aagggcctat
     1681 agctcaggtg gttagagcgc acgcctgata agcgtgaggt cgctggttcg agtccagcta
     1741 ggcccaccat ttgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc
     1801 ggtggttcga ttccgcctct gggcaccagg aatacggcgg tatagcttag ttggctagag
     1861 cgttcggttc atacccgaaa ggtcataggt tcgactccta ttaccgctac catctaacta
     1921 tatcaa
//
LOCUS       DAYATN010000258         1865 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.262, whole genome shotgun sequence.
ACCESSION   DAYATN010000258 DAYATN010000000
VERSION     DAYATN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.262"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(833..1219)
                     /locus_tag="ACGI8A_003721"
     CDS             complement(833..1219)
                     /locus_tag="ACGI8A_003721"
                     /inference="COORDINATES: protein motif:HMM:NF024502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-terminal phage integrase SAM-like
                     domain-containing protein"
                     /protein_id="HFH0181662.1"
                     /translation="MNNNKFVAPNNVTLVERCYRYIKDRECSFSPNTIRNRKNMIKNQ
                     IEVFFKDMKLIDITPSILQSYINNIYNEHMLNSIKNQVDFIKSVLKESYRLKEISENI
                     CDFVTTPIKKVVQLLSFIQNKKLNYY"
     gene            1252..1398
                     /locus_tag="ACGI8A_003722"
     CDS             1252..1398
                     /locus_tag="ACGI8A_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181663.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            1520..1654
                     /locus_tag="ACGI8A_003723"
     CDS             1520..1654
                     /locus_tag="ACGI8A_003723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181664.1"
                     /translation="MLDKRLGKRRLKNIYEDIEKENNLVKKFYNIRVESENMILKKNA
                     "
BASE COUNT          714 a          177 c          244 g          730 t
ORIGIN      
        1 acgaaactat taattaataa ttttatttct aatataaatg ataaactatg gtataatacc
       61 aatgtcaaca gcaaatagta tataattata aattttatta aataatagtg attttaaggg
      121 tcttataaat atatcttatt tatatattac tgacatttaa ctgacaaata ggaaaaaggt
      181 actttaagtg acatttaact gacaaatgaa aaaaaagcac tttgagtgac atttaattga
      241 cataaaactg aacattattt gaatagtatt ttgcttatat tttcagatgt ttctttattc
      301 atttcatcta ataagtgaga atatggaata tatattcata gctattttta tatctgcatg
      361 tactaatatt ttataaatag cttttatgtt agtacctgaa agtaatatca aagttgcatt
      421 tgcatgtatt aattcatgta cttttatatc tctaggatta ttatctctta atagtttttt
      481 aaaatgaaca acaaaagttg gttgaactca cgggttgaat cttttattta agcatatcaa
      541 atcaaattca tttttcaata taccctgtaa ttttaactta ttttgtttta atttttcccc
      601 tttaagcaaa cttataagtt catcaggagc aaataatttt ctttttgata taggggtttt
      661 agggtctttg aagattattt tattgtatac ataaactaat gtttgattta catgtgagat
      721 tttttatcaa ggtctacatc tgaccatata agtgatacag cttcaccaaa acgtaatcca
      781 agagttagca tcaaaaaaat tggtatacct ataggaatat tttttgattt ttctaataat
      841 aattgagctt tttgttttgt ataaagctta gaagttgaac tacttttttg attggggttg
      901 taacaaaatc acaaatattt tctgatattt cttttaatct ataagattct ttcaatactg
      961 attttataaa atcgacttgg ttttttattg aatttaacat gtgctcattg tatatgttat
     1021 ttatataaga ctgtaaaata gaaggtgtta tatctattaa tttcatatct ttaaagaaaa
     1081 cttcaatttg atttttaatc atattttttc tatttctaat agtattagga gaaaaactac
     1141 attctctatc ttttatatat ctgtaacatc tttctacaag tgttacatta tttggagcaa
     1201 caaacttatt attgtttata gtggactatt ggaaaatcga ataataataa cttggtattc
     1261 gaaacttttg ataaaacaat agaactatat ccaaatgcaa aacctatttt tcatagtgat
     1321 ataggattta gattagatga tgaaagtttt gaagagtatt taggaagagt atttagaaag
     1381 agatgcaaag ggatataaga ttgtaaataa aaatggattt tttaatgaat ttgattttta
     1441 tgaagcagta aatatatgtt taaatatctc aatagaagat gcgataaaat catataatat
     1501 attagttaaa tcattagtaa tgttagataa aagattaggt aaaaggcgat taaaaaatat
     1561 atatgaagat atagaaaaag agaataattt agtaaagaaa ttttataata taagagttga
     1621 atcagaaaat atgattttaa agaaaaatgc ataaaaaata caactaatac agaaataaat
     1681 aaagtaaagt tccatggaaa actgagaaat ttaaaaagag tatatatatt gaaatgtagt
     1741 aatttcaata tatacactct tttttatttt gaactaattc ctaaacgtca atttagagag
     1801 taattgaatt ttaagtttag ttaaaatgaa agttattttt actaaatatt atctttgttg
     1861 tatag
//
LOCUS       DAYATN010000259         1835 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.263, whole genome shotgun sequence.
ACCESSION   DAYATN010000259 DAYATN010000000
VERSION     DAYATN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.263"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            411..1739
                     /locus_tag="ACGI8A_003724"
     CDS             411..1739
                     /locus_tag="ACGI8A_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0181665.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          650 a          210 c          330 g          645 t
ORIGIN      
        1 atataaaata gactataaaa caagcaaaaa tcatactatt atataaaaga ccttaaagat
       61 agaaaactac agaaagaagc caaaatgttt ttgaaaaaaa tgaaatttta ttgcaaaaaa
      121 atcaaaatga atttaactta atagttataa tctattttta gtgttacaaa tatacaatgt
      181 atattataca atataaaaaa tacatttact tagaagtatt attatatttg gatttttggt
      241 ttttataatt tttttatatt taaacataac taaaaataaa agattactaa agctaagtag
      301 agtttatttt attttaataa ataataaagc tatttttcta tatattggca taatatatgc
      361 ttatttaact atgaaattaa aattaaatag gattaatgag gaggaaaaaa atgggaagta
      421 atgaactgaa aaagactctt ggcgtatcag cagcattatc aactgttgtt ggttccgtta
      481 taggagcggg cgtatttttt aaaccacaag cagtctatac attaactgga ggagctccag
      541 gtcttggtat attggcatgg ttaatagctg gaatcataac tataacagct ggtcttacag
      601 ctgcagaggt atcagttgcg ataccaaaaa ctggtggaat gatggtatat ataaaagaaa
      661 tttatggaga aaaattaggt tttttaactg gatggatgca aattgtatta ttttatccag
      721 ggatgatggc agcattaggt gttatatttg gagaacaagc atcggcacta attggtagtc
      781 catcactttt acttccaata gcaattggaa ttataataat agttgcaggg cttaatatgt
      841 taggttcaaa aactggagga gtcatacaaa ctgtttctac aatttgtaag ttaataccac
      901 ttattttaat aatggttgtt ggatttatta aaggtggtgg aaataatcca atacttacac
      961 ctatggttgg tgaaggatta agcctgggta gtgtactagg tcaagtttta attgctatat
     1021 tatttgcttt tgatggatgg atgaatgttg gtactttagc tggagaaatg aagaatcctg
     1081 gaaaagacct tccaaaagct ataataggtg gattgtcagt tgttatggct gtttatttta
     1141 taataaattt agcgtatctt tgggtattac cagctaatga attagcaaat tatgcttctc
     1201 ctgcatcagc agttgctgaa gttatatttg gttcaatggg tggaaaaata atatctgtag
     1261 gtatattaat ttcagtgttt ggagcattaa atggattttt actaactggt tcaagggtgg
     1321 catatacttt agctacagat aagactttac caaagtatgg tatattttct aaattaaatt
     1381 ctgctcaagt tccagcaaat gcaatagcat tagtatctgt tattgcatct atatatgctt
     1441 tgtcaggtca atttaattta ttaactgact tagctgtatt tgctacttgg atattctatg
     1501 tattaacttt tataggggtt atgaaactta gaaagactca tccaaatata ccaagagagt
     1561 ataaagttcc attatatcct attgttccta taatagctat agcaagtggt atatttgttg
     1621 tagttaatca attgtgtttt gcaggtatga aaactactat gatatctata ggaggtcttg
     1681 taataacagc tataggctta ccagtgtatg cttatatgac tagaggagta aaaagataaa
     1741 ccaagtagtt tacaaatgct tcataattaa aataaaagag actgtctcaa atagagataa
     1801 gtctcttttt tattgttaaa atggtaaaga tatgt
//
LOCUS       DAYATN010000260         1831 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.264, whole genome shotgun sequence.
ACCESSION   DAYATN010000260 DAYATN010000000
VERSION     DAYATN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.264"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            89..436
                     /locus_tag="ACGI8A_003725"
     CDS             89..436
                     /locus_tag="ACGI8A_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFH0181666.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(586..1635)
                     /locus_tag="ACGI8A_003726"
     CDS             complement(586..1635)
                     /locus_tag="ACGI8A_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0181667.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
BASE COUNT          619 a          284 c          249 g          679 t
ORIGIN      
        1 tttattccta ttgtgtaaag tgagaatact attttcttat attggtataa taatagtaaa
       61 tctaatatag aaaagaggta tttctagtat gcatgaaaat aaaaaaaatc tattagacgg
      121 aagcttagct caatataatg atacagcagt tccaaaaata ccagtatttg ctggtaacga
      181 tataacttca gaagtttatt attttaaacc taatcaagtt cttaatgctc atagacaccc
      241 taatggtgaa caaatatttg tgttcctaaa aggagaaggt aaaatgaaac ttggtgagca
      301 tgaatgtgat gtaaaaaacg gcgatactgt atttgtacca acaggagagt ggcatgaaat
      361 tactaatgga agcaatgaag aaatggtagc cgttcaaatt acaaaaatta atgctggagc
      421 agaatacaga ggatagatat ataagtacag ttagtacttt aatatctatt ttattatata
      481 aactttaatc tcaaatataa aaagagtctt ttaaaaggct ctttttatat ttttgttaat
      541 tttcattata aaaacaagaa aatatctagc taaaactaat atattttatt taatattttt
      601 tatagcattg tttgcaaact cagtatttac gatatcttta taaggtgctt ccttatctag
      661 ttctttagct tctttcataa cagtcatcaa tttcttcaaa ccttcctctt ttaatacagg
      721 attttgagac catgcatcta ttgatttata tctatctaca actgttgtta aatcttctaa
      781 agatatatct tcaaagaaag gttgcatagc cttagctact tctttgctag atgcttgttg
      841 aatccataat tgacctttat aaacagcatt tgtaaatctt tgaattaaat ctttattgtt
      901 tttcatgtat gatctcgtag tagagtatgc tgtaaaaggt atttctccag aatcttttcc
      961 tatagatgta actatatatc cactaccttc tttttccatt gctgttgctg taggttcaaa
     1021 tgctgttgta aagtcaccct ctccacctgc aaaagctcca gccattacac caaattgtac
     1081 atcagttctt acattaacat tgcctgcttt tagattttct ccaatagtaa gacctttttc
     1141 ttttagaaca tattcaaaag tcattagtgg aactccacct tttctaccac ctaatatttc
     1201 ggttcctttt aaatcattgt aagagaaatt aggcattttt ttcctagcaa ctagaaaact
     1261 accatctctt tttgtcaatt gagcaaagtt cactgcataa tcgtttttgt tttgattata
     1321 tacatatata gatgcttcag gacccattaa acctacgttt gcttcacctg aaatcaaagc
     1381 tgccatagtt ttatctgcac cttgggtatt tataaggtca atctttatac cttcatcttc
     1441 aaagaatcct ttcgtaatag cagcatactg tggagcataa aaaacagagt gagtaacttc
     1501 tgctatagtt actttatcca gatttttaga cgaagaagat tcaccttttt tgtcattaga
     1561 gttaaaatca catccagtta gacatattgt acttacaatg atagagcttg ctattgcgac
     1621 tattttttta aacataataa atacctccca taagacatac gaatagtata tagtattaaa
     1681 aatttattgt agttgttaca aaaatgtatc tatgcttata atatttctac aaaaacttaa
     1741 taataatttt cagctttatg tttagttttt tcagtaatgt gtaatattgg ataaattata
     1801 ttgattattt ataaactaag taataaaatt a
//
LOCUS       DAYATN010000261         1754 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.265, whole genome shotgun sequence.
ACCESSION   DAYATN010000261 DAYATN010000000
VERSION     DAYATN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.265"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(28..594)
                     /locus_tag="ACGI8A_003727"
     CDS             complement(28..594)
                     /locus_tag="ACGI8A_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFH0181668.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            755..1408
                     /locus_tag="ACGI8A_003728"
     CDS             755..1408
                     /locus_tag="ACGI8A_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFH0181669.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGFSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(1566..>1754)
                     /locus_tag="ACGI8A_003729"
     CDS             complement(1566..>1754)
                     /locus_tag="ACGI8A_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181670.1"
                     /translation="RDDFMYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIV
                     HKFKLYVVNEKYIKGIME"
BASE COUNT          586 a          219 c          223 g          726 t
ORIGIN      
        1 tttattttca gtcttacttt attaaaatta ttttttggtt atgtctggaa aatatctgtc
       61 tactaatttt tctgaaatat tttttaattg gtcagccatt gaagtcagac ttaataaggc
      121 tatcaataaa tcatctttat tttctggatt tatactaata ttttcaaaat tagactctat
      181 aaagtccggt atattctttc tttgattttc ttttattaac atgaattttt cgtaacatga
      241 aactaaatct ttttcactga aattatcctt ttcatacaca ccatgtaaga ttcttttaat
      301 ttcttgtatt gtaagtgtat ttttcataga ataaataatt atcatctgta aaatttgttc
      361 tttagtatac ttttttccct taactggttt gattaatccc tctttgctgt aattatttat
      421 catggtttta gtaagaattt tatcactttc aaatctttta ttggcagaaa gcttattgtc
      481 aattagagaa ataatttgat ccatatataa gtcaatagat ggaatatcgt ttgaagttat
      541 atctgcatta tttagtgttt ctagtattat agtattaagt tcattattca tcatatgtta
      601 cctccataat atatataata cactaaatta gaaagtaaaa caatattaag gcttgtaaaa
      661 aaaacataaa gtggtataat taaatatata atatagtaat ggaaactata tttgtgaatc
      721 tgatttttaa aatacataat tgtaggagga cgaaatgtat caattttttt taaagggtag
      781 agacccaata agcagtttaa ctcattttat aggtgcttgt ttatctcttt tagcaactat
      841 aattttagtt tttcaatctg ttacattaca ggaaacttct ctcttgatga tagtatctgt
      901 atcggtattt ggattttcac taattgcttt atatagcgca agttcgtatt atcatttttt
      961 aaaggggaca ccagagcaag aattgttttt tagaaaagta gaccatgcaa tgatttacgt
     1021 attgattgct ggctcttata caccaatatg tctgaatttt atggaaaaaa aagaaggcat
     1081 aatatttgtt acagcaatat ggatagttgc atttattggt ataataataa aaatattttg
     1141 gatggatgca cctagatggt tatctaccag tatatattta cttatggggt gggcaattgt
     1201 ttttgatata aatgcattca attcaattcc taaggattgc cttagactat taattatgga
     1261 aggtgtatct tactctatag gtgcaattat ttatataatt aaaaagccta atataagtcc
     1321 tgagtttgga tttcatgaaa tatttcatat ttttattatg ataggttctt tatttcactt
     1381 tttagcagtt ttattgtatg ttttatagtt ttaaaagatt tgattaattg atactaaaat
     1441 tctacaatga ataaaatgga actgccttat atagattata tcttcgacaa taagacagtt
     1501 ccttattata aatattaaat tctacttata ataaaatttt gataaaaata gtccattcaa
     1561 aatgcctatt ccataattcc tttaatgtac ttttcattta cgacatatag tttgaattta
     1621 tgtactataa ttatatcctc taaattctct gaaacactaa caattctgac acatttgttt
     1681 ctaatttcta caggtaaatc taaatcattt gttaaattca catagtcatc atatttatac
     1741 ataaaatcat ccct
//
LOCUS       DAYATN010000262         1681 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.266, whole genome shotgun sequence.
ACCESSION   DAYATN010000262 DAYATN010000000
VERSION     DAYATN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.266"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(69..635)
                     /locus_tag="ACGI8A_003730"
     CDS             complement(69..635)
                     /locus_tag="ACGI8A_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3073 family putative ECF transporter S
                     component"
                     /protein_id="HFH0181671.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            801..1322
                     /locus_tag="ACGI8A_003731"
     CDS             801..1322
                     /locus_tag="ACGI8A_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3072 family TudS-related putative desulfidase"
                     /protein_id="HFH0181672.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKIKDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
BASE COUNT          699 a          194 c          256 g          532 t
ORIGIN      
        1 aaaatgttat ataaaaatga gtatatagca ttttaaagag taataaacct tacataataa
       61 atgatttatt aatatgaact agaagtattt ttactatatt gttttataaa acctttaggc
      121 attttactta taaaatatac caccataata caagacaaag gtttgtctac tatattacta
      181 agaatccttg gaaagaacgc agcagtaaat atcttctgac cagactgaac taaaaaacca
      241 accaataaat cagcaccact tccacttaaa ccaccaaaca ataaaactga aataggagta
      301 ccaattaaag gacaaacaat agataaaatt actcctgtaa taatagcttc tttataacca
      361 aaaccttttt tcttagaaat atatccaaca ataagaccaa ggacaatatt tacaagggca
      421 aaaggtatat ttgtaggacc tgatataaca cctaaaacta agtttgtaca tccaccaata
      481 agagcacccc aaaaaggacc taatacgaca gccccaagta cagtaccaac tgtatctaaa
      541 aataataatg gtatatttat catagatata accataccta agacaatatt aataactatg
      601 cacattgaag aaaatgtgac ttttttagta tccaattata atacctccta aaaaaatata
      661 tacatttatc atagtataga ttataaaaga ttactaattg tatatttcta tatagaatat
      721 tggaaatata gaacaaataa ataatataat tatcaaaata tgtagtatca ttaaaattaa
      781 gaataactaa ggagattatt atgaaaagaa atagagagat tgtagtagta tgtcattgta
      841 tattaaattg taattctaaa gtggagggat tgtcagaatt taagggagca caaaatatag
      901 cgataaattt aataaataaa gggtatggaa taattcaact accatgccca gaaatgatta
      961 tgtatggaat aaataggtgg ggacatgtaa aagaacagtt tgacaatttg ttttttagag
     1021 aacaatgtag gcaaatgtta atacctcatg taaggcagtt tgatgaatat ttaaaaaatg
     1081 attatgatat aaaaagtatc attgcaatag atggcagtcc aagttgtggt tataataaga
     1141 catgtagttc taaacgttgg tttggtgagt taagtggttg caatgaattg atggataaaa
     1201 taaaggatat acaaatggtg gatgggaaag gtatttttat agaagagcta gaaaagctac
     1261 ttaaagaata taatttagag atacctatag ttggatttga cgagcttaga gaagataagt
     1321 agagatataa atatttattg tctcattatg aaacaccatt atatttggca gaaaaatatg
     1381 actgatggac agtacaggaa ccatttttaa gtggagtaat tagtacttat aaagatgtat
     1441 ccactagctg aaagaaaatg tagaaaggta ataaatgaat taagaagtag ataatatata
     1501 taaaggagtg tctaaaagtg aactttttag tttatgagac actctttttt tatattaaat
     1561 caagtatatt aaatcaaata tattttaatc attttagcaa tgaaaaataa atttatctat
     1621 attttgagac aacatctttt ttagttttat ttatttgttg tcgtttttga cttggtatac
     1681 a
//
LOCUS       DAYATN010000263         1670 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.267, whole genome shotgun sequence.
ACCESSION   DAYATN010000263 DAYATN010000000
VERSION     DAYATN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.267"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            219..1622
                     /locus_tag="ACGI8A_003732"
     CDS             219..1622
                     /locus_tag="ACGI8A_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HFH0181673.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT          704 a          185 c          312 g          469 t
ORIGIN      
        1 attaacataa attattttga acttataact taattaaata aaataaatta taatgttttg
       61 aatttataga gaataaaaac tttaggtata ttgtttgagt tgtatggata actatggtat
      121 catatataaa atatggtaac aaaattgtaa cttttgatta aaaataagta tgagtactaa
      181 taaaaaatat gaaaagtaca ttgggggtag aaaataatat gaagaatagt aaaaagatat
      241 tagctatagg acttacacta tttttagtaa tggtaaatac tcctatggta aatgcgttga
      301 catcagttga gcaaataaaa gggaatgaca gatatgagac agcagcaaaa atagcagata
      361 agcaaaacta caatacagca atactaatca actcagataa tagcttagca gatggtctaa
      421 gtgcaagtgg tttagctgga gccttaaatg cgcctatttt gatgacaaaa caaaatcaga
      481 ttccaaacac aactatggaa agattaaaca aagcaaagac tgtgtatata ataggctcag
      541 aatcaacaat aagtaaaaat gttgagaatc agttactatc caaaaagaag gttgtacaaa
      601 gaatctttgg tgaaaataga tttgatacaa gtataaaaat agctgaaaaa atcaaagaaa
      661 ttaagccaat agataaagta attatagcca atggatttac aggagaagca gatgcgataa
      721 gtgcatcacc agtagctgct agagatggag tacctataat acttacagat ggaaatagtg
      781 tgggatttga cacgacaggc ttaaagagtt atgcgcttgg ttctagtgaa ataatcagcg
      841 acgaattagt taagagcaca aattctatta gattgggtgg cacagataga tttgaaacta
      901 ataagatagt tatacaagag ttttataaaa atagtaaaga attttatcta agtaaaggct
      961 tacaattaac agacgctttg gctgcctcta caatagcaaa aaatgctcct gtagttttag
     1021 ttgaaaatgg cagtaacaag agtattttaa gtggtgcaga caaattgact gtactaggag
     1081 gcataaatca gaatgttatc aagcaatgta taaatcaagc ttcaccaaat caacaaggtt
     1141 tatattacaa tccaaatgat agagcattta aagagagaat aaagggtaaa gtatatgctc
     1201 ttacaaagca gtatagaaaa gagaatggag taagagcgtt atctgtagca agcagattag
     1261 agggtttagc aaatgattgg tctaatttaa tggcaaataa gaagacttta tctcatacga
     1321 taaatggaaa aaattcatat tctacttttt tgaaatactt agattggagt gagattaaac
     1381 ctggatatat agcagttcaa ggtgaaaata taattaaata taaaattcca gataaacctg
     1441 tatatacaaa tagagatgca gatgatatag gaaactttat ttttaatgag tggaagacaa
     1501 atccagaaga aggaactaac atgttgcata aagggtatga aataatggga tttggtatag
     1561 ctataacagg agataagaat ctatatgcta cacatgaatt ttatggaaga tataaagaat
     1621 aagaaaatat acacaaaatt atgcttgtaa ggaagctata taaataatta
//
LOCUS       DAYATN010000264         1662 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.268, whole genome shotgun sequence.
ACCESSION   DAYATN010000264 DAYATN010000000
VERSION     DAYATN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.268"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            43..1641
                     /locus_tag="ACGI8A_003733"
     CDS             43..1641
                     /locus_tag="ACGI8A_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFH0181674.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
BASE COUNT          641 a          180 c          290 g          551 t
ORIGIN      
        1 atagtttcaa atatatatcc taacttatag ggggttttaa ttttgaaagc tgcaatttat
       61 tcaagaaaat caagatttac tggaaaaggt gattctatag aaaatcaaat acaattatgt
      121 attgattatg caaagtctat tggtgtaaaa gattttttga tatatgaaga tgagggattt
      181 agtggtagca atatagatag accacaattt aagaaaatga tgcaggatgc caaagataag
      241 aaatttagtt acctagtttg ttatagatta gataggatat cgagaaatgt atctgacttc
      301 tctactttaa tagaaaaatt aaatgaacta agcatatcat ttatatctat caaagaacaa
      361 tttgatactt caactccaat gggaagagct atgatgttta tatcaagtgt atttgctcaa
      421 ttagaaagag aaacaatagc agaacgtata aaagacaata tgtatgaatt agcccgtagt
      481 ggtagatggc ttggaggcaa tacacctcat ggattcaaca gtgaaaaaat cacattctta
      541 gatgaaaatc taaaagaaag aagtatgtat aagcttcaag taaatgatga acaaatggag
      601 attgtaaagc taatatacaa taaatattta gaacttaggt ccttatctaa gttatataaa
      661 tatgtctata ataatggaat aaggggtcct cgtggtggca aatttgaccc tagttcttta
      721 tctagaatac ttagaaatcc agcatatgta aaagctaatg atgaaatttt agattacctt
      781 aaaaatagtg gtatggatgt tgtaggtact ccagatagaa aatgtggaat ccttacctat
      841 gctaaaaata catctaactc aatagcagca attgcaaaac accatggagt catagaacct
      901 gaaatatggc ttgaagtaca aaatcaatta gataaaaatc gtgataaaac tccaaggata
      961 tcaactggaa aaactgctct tttatctggt cttataaaat gtggtaaatg tggttctaat
     1021 atgagaatta cctataaagg caaaaaaact gacaaggatt taaagtatta ttatatttgt
     1081 ggtactaaaa aaacacttgg gtctgatagt tgcaactgta aaaatcttaa tggtccatta
     1141 gttgaaagtt tagttataga taaaattaag aattgtaaag aagagtctat tataagtgca
     1201 ttcaatagta ataacattaa gactgataat gtttttaata tttttaaatc tgagactaat
     1261 ttacttaaaa atcaaatttt agataaggaa agacttatta aaaatttagt tatagagctt
     1321 gcaaaaaata ctggtagtac tgcttctgat tatatagttt cccagattga gagcctaaat
     1381 aatgagatta gtattttaaa ggataagatt aatactttgg aatctagtaa catcaatatt
     1441 agtagtgaca gtattaatat tgatattatt gttagtaatt tgaagagatt taatgatgaa
     1501 gttgataatg ccagtttaga tgataagaga ttgttattat ctactattat taatagtatc
     1561 gtttgggata gcagtttagg aaatcttaat attgtttata tgggtgctga agttgataat
     1621 tctgtttctt taagcaatta gttgcacttt tttagctata gc
//
LOCUS       DAYATN010000265         1624 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.269, whole genome shotgun sequence.
ACCESSION   DAYATN010000265 DAYATN010000000
VERSION     DAYATN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.269"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            58..687
                     /locus_tag="ACGI8A_003734"
     CDS             58..687
                     /locus_tag="ACGI8A_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HFH0181675.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            755..1588
                     /locus_tag="ACGI8A_003735"
     CDS             755..1588
                     /locus_tag="ACGI8A_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HFH0181676.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNCA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
BASE COUNT          619 a          207 c          245 g          553 t
ORIGIN      
        1 taataagaaa actttttata aatatacttt taattgttta aattggagga aaaaattatg
       61 gatgaaaaga taacaaaaaa taattataat gttccatttg actatgttcg aaaaaaactc
      121 aaagaattag accctcatac aattagtaat ttaagtaata tacctttcaa taatgattta
      181 aatagattca cactcaattt ttttgataga caatatatta tagactatcc aagtggagaa
      241 gtttggaata gtaatggtag cctttgcaat aactttgagc ttaaaatctt gatagttaga
      301 tatttaatca atgctaaagg tattcctaga actaataaat atgtaacttt taaagaaata
      361 ccaggaggca atgtttatta tccaaatttt ttaaacagga ctctatatag gctttcagaa
      421 gtttttacat ctcaaataga caaatataag ttagttatgg agaaaattgg agctgaaaaa
      481 gtaaacatgg gagatttagc ttataggttt acatatatgg gtaatacatc tatggtattt
      541 atactttggt ttggagatga tgaatttcct ccaaattcaa atatattatt tgattctaat
      601 atagtttatt acttcaatgc agaagattta gcagttgttc ctgatacagc tataaatgca
      661 attttaaata aaataaatca attataaata taaataatat tataataaag tttttgatag
      721 atttaataga atataatatg aaatggagta aaaaatgaat gtagaaaatc aatctttatc
      781 agatattgaa ataatattat cacacaggta tgataatggt gcagactatt ggactacacc
      841 tgataagcgt ttaataaaag gttctccttt ctctactctt gatagtgtac tttatctatt
      901 agaattaggt gtgaaccctg atgatacttt acttaaagaa tgtgctgact tgatatttag
      961 cacatggcga gaagatggac gtttcaaact atatccaaat ggaggtattt acccttgtca
     1021 tactgcaaat gctgctaacg tactctgtca tatgggatat gcatctgaca ttagaatgca
     1081 aaaaacgttt caacattttt tagatacaca gtatactgat ggtgggtggc gttgcaataa
     1141 atttagtttt ggtcgaggtc cagaaactga atactcaaat ccactaccaa ctcttaatat
     1201 attaaatgca ttccgtttta gtaattatct aaataaagaa tcaaaacttg acaaagctgt
     1261 agattttcta cttgaccatt ggactattaa aaaaccaatc ggcccttgtc actatggaat
     1321 tggaacctta tttatgcaag ttgaatatcc tttccgtaat tataacctat tcctttatgt
     1381 ctacgtttta tcattttata attgtgcaaa aaaggataat agatttttag aagcattgaa
     1441 gatacttgaa tctaaaatga taggtaataa aattgtcgtt gaacgtgtag ttccaaaact
     1501 atctaaatta tctttttgta aaaaaggcga gtcaagtgaa atagccacaa cacactatta
     1561 cgaaatatta aaaaacctag aaaaataata gttatataga taaaacatat taaaaaatag
     1621 tggc
//
LOCUS       DAYATN010000266         1615 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.270, whole genome shotgun sequence.
ACCESSION   DAYATN010000266 DAYATN010000000
VERSION     DAYATN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.270"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(112..1023)
                     /locus_tag="ACGI8A_003736"
     CDS             complement(112..1023)
                     /locus_tag="ACGI8A_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFH0181677.1"
                     /translation="MAVISFCILHLITVSTISLVKNTSPDIIFANMTYKLISILIASI
                     LDFILWYSMNKIKEHIYIITQNQIEFSLFLKFIWFTLGYILFDSIQITFAPPSSLTSY
                     FILGSNILLILQLIIFINHIYSIEKNIYLEKEHNRLKMIKQEQLLKVNLLKKKANIDE
                     LTSAYNRKFAFEKIQHYIDNNLLFSLIYIDLDKLKTINDTYGHLAGDNYIINFSSIVI
                     ENLGIDDIFARIGGDEFIVIMPKSNENTTKEFIEKISKIMDNTISFSYGIVEVSSENK
                     MTVEEIINIADIRMYTNKQNNHKRNNN"
BASE COUNT          577 a          160 c          203 g          675 t
ORIGIN      
        1 tttttagagt tattaatcat tatgtccaga tatttaaaaa aattataatg atagagataa
       61 taagataaaa aacatagtga gactacaagt aagctcatgt aaatattcat tttagttatt
      121 gttcctttta tgattatttt gtttattagt gtacattcta atatcagcaa tattaataat
      181 ttcttctaca gtcattttgt tttcgctaga aacttctaca ataccatagc taaaagatat
      241 cgtattatcc ataattttac ttattttctc tataaattct tttgtggtgt tttcattaga
      301 tttaggcata ataactataa actcatctcc tcctattcta gcaaatatgt catctattcc
      361 taagttttca ataactatac ttgagaagtt aataatatag ttatccccag ctaaatgtcc
      421 atatgtatca tttatcgtct ttaatttatc caaatcgata tatatcaatg aaaataataa
      481 attgttatca atgtaatgct gtattttttc aaaagcaaat tttcgattat atgctgatgt
      541 taattcatca atgttagctt ttttctttaa taaatttact tttagtaatt gttcttgttt
      601 tatcattttt agtctattat gttctttttc taaataaata tttttttcaa tggaatatat
      661 atgattgatg aatataatta gttgtaagat taataatatg ttacttccca atataaagta
      721 ggaagttaga gaacttggtg gagcaaatgt aatttgtata ctatcaaata atatatatcc
      781 taaagtgaac cagataaatt ttaaaaatag actgaactct atttgatttt gagttattat
      841 ataaatatgc tcttttattt tattcataga gtaccataag ataaaatcta aaatagaagc
      901 tattaaaata ctaattaact tataggtcat atttgcaaat attatatctg gacttgtatt
      961 tttaacaagt gatattgtac ttacagtaat aaggtgtaaa atacaaaaac ttattactgc
     1021 catattggga ataaaatggt tgttagtaga actttttctg cttaagagca atttatgact
     1081 tactatgtaa aacaatatat aatatagtgg taatggtaat tttgtgaata ttattatcaa
     1141 gaaggttaat ggttttatta aaaaaatata tttgtaattc ttatgtatag ttatattttt
     1201 gtttccaaaa gatgctattt tgcatatgta aatgttacat agttcgatta ttaatatagt
     1261 agttatgatt aaaaatatat ggtatttcat agtacatcct cttttaaagt gaaattgtat
     1321 ttacttaaaa tagattagat tttatatatt ctattttaca gtttttattt aggtacatgc
     1381 aaagagctta ttatggatac cttctttttt attgcatgtg tctgtgttat ataaaattta
     1441 aaaagtatta tatcttaata tacagtatgt atgataacta tgtgtccaaa tatagtaatt
     1501 atgtatggaa agtataatag ttatgtgtat aagtatagta attacatata gcatgtatga
     1561 tagttatgtg tataagtata gtaattacat atagcatgta tgataattat gtgta
//
LOCUS       DAYATN010000267         1558 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.271, whole genome shotgun sequence.
ACCESSION   DAYATN010000267 DAYATN010000000
VERSION     DAYATN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.271"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            193..1533
                     /locus_tag="ACGI8A_003737"
     CDS             193..1533
                     /locus_tag="ACGI8A_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFH0181678.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          608 a          121 c          251 g          578 t
ORIGIN      
        1 tattattaaa aaaagcaata aaaagtttaa ttcaatttag caaatttagt gatataatta
       61 aaaaagaaaa gaaatatgga cttatttgga ataattttga caatctattt actacatgtg
      121 tagagtcgta tataattaaa ttatacataa aatataaaaa aataatttag taatacgtta
      181 ggagaaagta ttatgataaa gtcaagctta ttttggaata ttttcattgc gatatccttt
      241 atgatagaat ggtttctttg taagaaaatg ctagattata caagtgagaa aaggatttct
      301 aacaaaaaaa taaacttttg tatggtaggt attattgttt ttgctgacat aatatatttt
      361 tttgatgtac ctccaaatat aagagtaatt atatctatga taattactat tgcattttat
      421 aaaatctcat atgatgtggg gtttataaag tctgtattaa tatccttaat ttattggatg
      481 ttactaatcg ccacagatgc tttgagtata tctttaacag tttggcttaa ttctataaat
      541 gacatgagta gtatggggac gggagatata tacagaattg agtctataat attgggaaag
      601 atacttttga ttgtgtcttt gcttttatat agagccttaa agataaaact taatatagaa
      661 ataaataaaa ggacattatt ttatatagtt attcctatag tcgctaatat agcatctttt
      721 tttgtaattt ttgaatacat atttaatcca gggtctggta gcttaataca taatttccaa
      781 ttcgttatga tttctgttgt attgcttata tcaagtatca ctttagtttt aattgctaaa
      841 aaattacagc aatataatac tttagttgta gaaaacaata tgattaaaaa tactttgaag
      901 tatgaaaatg agtattatga agatatagaa aagaaatatt taaaaactag attactaagt
      961 catgatatga aaaatcacat attgtgtata aatgctatga ttaaaaaagg tattgatgtt
     1021 acttcttatt taaataattt gcaggtagaa attcagagta atgacttgtt atttaatact
     1081 ggtaatttta tattagatgc tatattaagt gaaaagaaag aggtttgtga taagaatgat
     1141 atagaactaa aaataggaat aaattttagt tgttgtgaag ctgttgaatt agtggatata
     1201 tgtcatatat tttcaaatat tttagacaat gcaattgagg catgtaataa aattgatagt
     1261 ggaaatagag ttatttgtgt taaaggtgat gttatgcaaa atttttattt aataagagtt
     1321 gaaaatacta agataaataa gattattact aaaaatggag atattttgac agacaaaaaa
     1381 ggttctcttc atggacttgg aataaaaagt gtaaaaaact ctgtaaataa gtatggtggt
     1441 aatgttgtaa ttgaatatga tgataaaaaa tttgtagtta aaattgctat tccacttaga
     1501 aatgtgaaga aagcagttaa tcatgtgatt taaatgtata taaatttgat gtgtagaa
//
LOCUS       DAYATN010000268         1513 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.273, whole genome shotgun sequence.
ACCESSION   DAYATN010000268 DAYATN010000000
VERSION     DAYATN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.273"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            208..429
                     /locus_tag="ACGI8A_003738"
     CDS             208..429
                     /locus_tag="ACGI8A_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HFH0181679.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            429..692
                     /locus_tag="ACGI8A_003739"
     CDS             429..692
                     /locus_tag="ACGI8A_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HFH0181680.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            754..1326
                     /locus_tag="ACGI8A_003740"
     CDS             754..1326
                     /locus_tag="ACGI8A_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFH0181681.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
BASE COUNT          602 a          171 c          268 g          472 t
ORIGIN      
        1 tataaactct atattattta tttaaaatag atatttatga aagattaatt agaaaaattt
       61 tagaggtaaa ataagaaaaa aagatactta tttaatcatt taatcttatt aaagacctat
      121 taaaactacc agttataata tagaaccagt atacaatttt taaaaataat gctattttaa
      181 aattctaaaa attaaggagg ataattaatg ggatatttaa aagaggataa acaagactat
      241 tgttgtgaat ttgctagacc atacatcaat cgtcaaattt atgatgacac atacaatcca
      301 aaaacagcat taaaaagagg tactttattt cctgaattag atttaataga gtcaaataac
      361 tataatgatt ggctatatgc aaatcctaaa aatagagtta gagctaaaaa agggggtaac
      421 atgtactaat gtcattaaat agaagagaac ttttagagag aatttcagaa tatcaatttg
      481 cctgtataga attaaattta tatttagata acaatccaag ggataagaaa gctcttgatt
      541 catacaacag atactgcgat aaatttacac aagctgtgtg tgattatgaa tctaaatatg
      601 gagcattaac taattttgga tatgaatcaa gtgaatatcc ttggtcatgg atatctgaac
      661 cttggccatg ggacaaatca ttttataaat aaaattatta aactttaaaa ttagatttat
      721 tattaaatat atattaagga ggagtaagat aatatgtgga tttatcaaaa aacactggaa
      781 catccagtta acataagaca agcagaccct agaatggcaa aatatatcat gactcagttg
      841 ggaggaccta atggtgagtt ggcagctgca actagatatc ttcaacaaag atatactatg
      901 ccaactggaa aatctcgtgc acttttaact gatataggta cagaggaaat ggctcatgtt
      961 gagataattt cttcagtgtt atatcaatta ataggcaatt gtactccaga agagcttaag
     1021 gctgctggac ttggtagtaa ttatgctaat tttggacatg gtcttcagcc agtagattct
     1081 aatggagtaa actttactac aagttatatt aatgtctttg gcgattcggt aactgattta
     1141 catgaggata tggctgctga acaaaaagca ttggctacgt actatcaatt aataaattta
     1201 actgatgacc ctgatttgaa agatatattg agatttttgg gtgagaggga agtagttcac
     1261 tatcaaagat ttggtgaagc attaatggat gtttatgagt ttacagagtg caagcatcag
     1321 ttttaatgaa gaattgaaag tgaatggaga ataagcatca gttttaataa agaattaaaa
     1381 gtgaacagag aataagcatc atttttaatg aagaatcaaa agtgaatgga gaataagcat
     1441 catttttaat gaagaattaa aagtgaatgg agaataagca tcatttttaa tgaagaatta
     1501 aaagtgaaca gag
//
LOCUS       DAYATN010000269         1493 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.274, whole genome shotgun sequence.
ACCESSION   DAYATN010000269 DAYATN010000000
VERSION     DAYATN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.274"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="ACGI8A_003741"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="ACGI8A_003741"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            163..237
                     /locus_tag="ACGI8A_003742"
     tRNA            163..237
                     /locus_tag="ACGI8A_003742"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:195..197,aa:Asn,seq:gtt)
     gene            243..328
                     /locus_tag="ACGI8A_003743"
     tRNA            243..328
                     /locus_tag="ACGI8A_003743"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:277..279,aa:Leu,seq:taa)
     gene            344..419
                     /locus_tag="ACGI8A_003744"
     tRNA            344..419
                     /locus_tag="ACGI8A_003744"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Met,seq:cat)
     gene            427..501
                     /locus_tag="ACGI8A_003745"
     tRNA            427..501
                     /locus_tag="ACGI8A_003745"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:460..462,aa:Glu,seq:ttc)
     gene            516..589
                     /locus_tag="ACGI8A_003746"
     tRNA            516..589
                     /locus_tag="ACGI8A_003746"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:548..550,aa:Gly,seq:tcc)
     gene            595..670
                     /locus_tag="ACGI8A_003747"
     tRNA            595..670
                     /locus_tag="ACGI8A_003747"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:628..630,aa:Val,seq:tac)
     gene            676..752
                     /locus_tag="ACGI8A_003748"
     tRNA            676..752
                     /locus_tag="ACGI8A_003748"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:710..712,aa:Asp,seq:gtc)
     gene            763..837
                     /locus_tag="ACGI8A_003749"
     tRNA            763..837
                     /locus_tag="ACGI8A_003749"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:795..797,aa:Gly,seq:gcc)
     gene            847..920
                     /locus_tag="ACGI8A_003750"
     tRNA            847..920
                     /locus_tag="ACGI8A_003750"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:881..883,aa:Arg,seq:tct)
BASE COUNT          465 a          271 c          321 g          436 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgttaaat gttccaaggt agctcaacgg
      181 tggagcaacc ggctgttaac cggtaggctg tgggttcgag ccccaccctt ggagccaaaa
      241 aagccgaagt ggcggaactg gcagacgcac aggacttaaa atcctgcggg acttacctct
      301 cgtaccggtt cgattccggt cttcggcact tataaaacaa tatcgcgggg tggagcagtt
      361 ggcagctcgt cgggctcata acccgaaggt cgtaggttca agtcctgcct ccgcaaccat
      421 taaaacggct cgttggtcaa gaggttaaga caccgccctt tcacggcggt aacaggggtt
      481 caattcccct acgagtcacc aattcaatta aatatgcggg tgtagctcaa tggtagagtt
      541 ctggccttcc aagccagctg tgagggttcg atccccttca cccgctccaa aaatgggacc
      601 atagctcagc tgggagagca cctgccttac aagcaggggg tcacaggttc gagccctgtt
      661 ggtcccacca ttcgcggcct ggtagttcag ttggttagaa tgccagcctg tcacgctgga
      721 ggtcgagggt tcgagcccct tccaggtcgc caaattaaat aagcgggaat agttcagtgg
      781 tagagcgcaa ccttgccaag gttgaagtcg cgagttcgaa tctcgtttcc cgctccataa
      841 taacttgggt gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg
      901 ttcgaatccc tgtgcgctca ttaagaaaag tttactataa attatagtaa acttttatat
      961 tgtttaaaaa gacacaggaa acatgtatat tacctgtgtc tttttattta taaactttaa
     1021 actatgtaaa aattacagta tattctaata gagttacagt atattcatta atattgtaaa
     1081 catacggagg gtaatgtata atagtctaca agatgtttat gaaggcatca gttaaatata
     1141 ataaatataa ctaattagta agagttatat acattatatt gtatacttta tggtatttat
     1201 atattgaaat aacttatgaa attatttata aagagattca tgagcatatt tttgtctaaa
     1261 caataagtat aagaaattat aaaaaattag agactatagt gaatttacaa tttaatgtaa
     1321 tatttataag ttatactaaa taaattatta tactgtgaaa tggatatata actttaggta
     1381 ttgtgtatta ttttcactgt aggagattat atctagttat acataataag ttttaatgat
     1441 agtttatagt aagatattaa aataaattta tctaatctag atatgaaata agt
//
LOCUS       DAYATN010000270         1489 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.275, whole genome shotgun sequence.
ACCESSION   DAYATN010000270 DAYATN010000000
VERSION     DAYATN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.275"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            30..1310
                     /locus_tag="ACGI8A_003751"
     CDS             30..1310
                     /locus_tag="ACGI8A_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFH0181682.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
BASE COUNT          628 a          194 c          170 g          497 t
ORIGIN      
        1 aaaataatta aagataaaag gagtatttta tgaacatact tggattaata gttgaatata
       61 acccatttca taatggacac atatatcatt tatctaaatc tatagaaaaa acaaatgcaa
      121 cacatactgt agcaataatg agtggaaatt ttctacaaag aggcgaacca gctttatttg
      181 ataaatacac tagagcaaaa attgctgtcg aaaatggtgt agacttagtt atagaattac
      241 caactttatt tgcttgtcaa agtgctgaaa ttttttctca tggtgctata acaattttaa
      301 attcactaaa ctgcataaat tctatatgtt ttggtagtga agaaggaaat acagaaattc
      361 tttataatct agctgaaatt ttagcaaatg aaccaaatga atttaagtta cttttaaaaa
      421 aatatttaga tgatggtctg ttatttccaa cagctagagc taatgcactt tttgattata
      481 tacatcaaga caaaaaaaat aattttatac aaaattattc aaaggaacaa ttatcaaaaa
      541 cattaaattc atcaaacaac atactgggta tagaatacat aaaaaacctc atccatttaa
      601 acagtagtat aaaaccattt actatctcca gggtccaatc agaatataat tccgaagaaa
      661 ttgagagtga tatttgttct gctactgcta taagaacttc acttaaagaa aatacagacc
      721 tttttcattt aaaaaatgtt attccagaaa aaacttataa catcctaaat gaaaaaatag
      781 ataatggttt tttacctatg tttgatgact tcttctttga tattttaaaa acaattatac
      841 ttagagatat ggacacttta aaagagtatt ttgaagtaaa tgaaggcata gaaaacagaa
      901 tctataaaga tatctttaca tctgaatttc tacatgaact acaattagca attaaatcca
      961 agcgatacac tttaaccaaa atcaaacgaa ctctaaacaa tatattgtta ggtattaaaa
     1021 aaagcgacgt gcaacttata aaaaacatag atactatacc ttatataaga atacttgctt
     1081 ttaatgataa aggtagagaa attctaaaag agataaaaaa taaatctgaa ataaaaataa
     1141 taaacaagtt ttcaaatata tcattttcat tagatgatac gatttttaaa acactcataa
     1201 gttatgatat aaaagctaca aatatatata attcaatata ttataaaaac aataaagatt
     1261 tagtaaaagg ccctatggat ttttatactc atccaatata tgtaaagtag ttttttaaaa
     1321 taactatatt tataaaaatc attttattaa ctctttatta tataagatac aatagtctta
     1381 ctttatttat gtacacatgc ttttaggatg acaacatcaa cttaaatatt atgttacttt
     1441 ctaaatttaa gtaaatattg ccatcctaaa aattttacaa tttagcttc
//
LOCUS       DAYATN010000271         1441 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.276, whole genome shotgun sequence.
ACCESSION   DAYATN010000271 DAYATN010000000
VERSION     DAYATN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.276"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            240..1067
                     /locus_tag="ACGI8A_003752"
     CDS             240..1067
                     /locus_tag="ACGI8A_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HFH0181683.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT          542 a          155 c          215 g          529 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatgt
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttggtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattacttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 a
//
LOCUS       DAYATN010000272         1343 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.277, whole genome shotgun sequence.
ACCESSION   DAYATN010000272 DAYATN010000000
VERSION     DAYATN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.277"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            167..877
                     /gene="rgaR"
                     /locus_tag="ACGI8A_003753"
     CDS             167..877
                     /gene="rgaR"
                     /locus_tag="ACGI8A_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HFH0181684.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          532 a          109 c          210 g          492 t
ORIGIN      
        1 ataggaattt atcgtaattt aagtttaaat attaaagaga atttctataa gtagtttaat
       61 cgacaattta aatttataaa gaaatttaaa tttgaaagct agtgagtaca gtttggaaat
      121 tgaaataaag taaactaaat gatattttag atttggaggt gatattatgt tcagggtagt
      181 tatatgtgat gacgaaaaaa tacaaagaag catactaaaa gagtttacag aaaaactttt
      241 gtctgaaaga tgtctaaatt atcaaatact ggaattttcc tgtggagaag atttaatatc
      301 caaataccca gaaaaaattg atattatatt tttagatatc caaatgaaag atatcaatgg
      361 aattgaggtg gctaaaaaaa taagaaagtt tgatgaaaaa gtagaaatta tatttacaac
      421 ggctttttca gagtatgccc cccaaggtta tgaggttcgt gcttatagat atttagtaaa
      481 gcctatagag tatagtaact ttgcaatggg tgtaaatctt tgtattgata accttcaaag
      541 aaagaaggag cgttatatag ttttaaatag taaaaaagga tttaatagaa tactgataga
      601 ttctatttta tatgttgaaa ctgtaaagag agatttagtc gtacatacaa taggtagaga
      661 ttataaagct aatatgagca tgaaacaagc agaaaaatta ctgtgtgaaa atggcttttt
      721 tagatgtcat acaagtttta ttgttaattt aagaagaata gaagatctta gggataatat
      781 gattactatt aatgaacaat ttatacctat aagtaagtat agacttaaag atttaaaagt
      841 agcattaaca agcttgttat gtgatataat gtattaattt taaaaaggta attgaaaata
      901 tcgtaaaaaa gatttaatgt catcacaggg ctattgattg aaactgtctt aatatataaa
      961 atgttattta tattaagaca gttttattat gcaaaatagc agtttataga tttttaatat
     1021 ggtttaacat gtatatgtag aggttccatt atagtagcat gattattaat tagtagcttg
     1081 attatcaata tactattatt gatttttata tgcattatat gtatttttag aatatgatta
     1141 cctttttaaa ataatgatta tttataaaac tctatagata tttgtttcat ttagctacgc
     1201 atactttaat taattatatt aatatttaat ggagtacaat ataaatatta aagcgacatc
     1261 ttatagttta ttgaaactat agtaatgaat taataaatag tttaattcta tgaaaagatt
     1321 tccttttatg caatttattt att
//
LOCUS       DAYATN010000273         1337 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.278, whole genome shotgun sequence.
ACCESSION   DAYATN010000273 DAYATN010000000
VERSION     DAYATN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.278"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            27..101
                     /locus_tag="ACGI8A_003754"
     tRNA            27..101
                     /locus_tag="ACGI8A_003754"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="ACGI8A_003755"
     tRNA            116..200
                     /locus_tag="ACGI8A_003755"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="ACGI8A_003756"
     tRNA            210..293
                     /locus_tag="ACGI8A_003756"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="ACGI8A_003757"
     tRNA            323..399
                     /locus_tag="ACGI8A_003757"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="ACGI8A_003758"
     tRNA            407..482
                     /locus_tag="ACGI8A_003758"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="ACGI8A_003759"
     tRNA            571..659
                     /locus_tag="ACGI8A_003759"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="ACGI8A_003760"
     tRNA            663..738
                     /locus_tag="ACGI8A_003760"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="ACGI8A_003761"
     tRNA            745..818
                     /locus_tag="ACGI8A_003761"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="ACGI8A_003762"
     tRNA            833..909
                     /locus_tag="ACGI8A_003762"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="ACGI8A_003763"
     tRNA            933..1009
                     /locus_tag="ACGI8A_003763"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="ACGI8A_003764"
     tRNA            1017..1093
                     /locus_tag="ACGI8A_003764"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="ACGI8A_003765"
     tRNA            1102..1177
                     /locus_tag="ACGI8A_003765"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="ACGI8A_003766"
     tRNA            1185..1258
                     /locus_tag="ACGI8A_003766"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="ACGI8A_003767"
     tRNA            1265..1336
                     /locus_tag="ACGI8A_003767"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          321 a          309 c          355 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAYATN010000274         1336 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.279, whole genome shotgun sequence.
ACCESSION   DAYATN010000274 DAYATN010000000
VERSION     DAYATN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.279"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            136..945
                     /locus_tag="ACGI8A_003768"
     CDS             136..945
                     /locus_tag="ACGI8A_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0181685.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            987..1253
                     /locus_tag="ACGI8A_003769"
     CDS             987..1253
                     /locus_tag="ACGI8A_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFH0181686.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
BASE COUNT          523 a          157 c          187 g          469 t
ORIGIN      
        1 agattttact ttacaattat tgtatctaaa tttaacaaaa tatcttatta gaatatttca
       61 attaaaacaa aactggttta ttatataaaa aataaattag tatacttatt ttttatatat
      121 caggaggata tacttatgaa taaaataatc aataacattt taaatacctg caaacagtat
      181 acatatattt tgccacataa attgttaaga cagtatatag cacactatac aatatctatt
      241 ccagatacaa gtattaaaga aaatcttaca ttaatacctg atgctagtgg atgtatgata
      301 tttaaatttg acaaaaaagg tatagaaagc gcattctggg gagccacaac aaaaactact
      361 attgtaaaaa atgatataga aaatgtactt tttagagtat tcgttgagtt tcgcccaggt
      421 ggagtatatt atttgacagg tctttctcaa agagaatcta cagatttaaa aatttcactt
      481 gaagatttta acacattatt tagcttagag gttaattcca tttttgaaag aacaagtact
      541 ataaaggaat tagtagaaca attagatatg ttattcttat catacttatt gaaatcaaat
      601 atagtagata tgactatacc aattcttgaa aatgctagaa aacaaaactc tattatgtct
      661 gttaaaaaca tttcacaaat tagttgttat agtgaacgac atttaaatag aatttttaat
      721 aattctcttg gtatgagtgt taaatcatac cttagattac ttcgtataaa ccttgtatta
      781 caagaaatac aaaataataa aataccattt gcaactttag cacaagatat tggttattac
      841 gaccaatctc attttataaa tgattttaaa tctatttgtg gtgttaatcc aacaacctat
      901 ataaaaaatt tgtccgattt ttacaatgaa aagtataaat tttgatatac aatcatataa
      961 aatttatatt taaggagtga cttaaaatga tagaatcaag atgtggaatt ttatgttatg
     1021 aatgtggata caaggaaaaa gtcaattgta aaggttgtac agagattgag aaaccatttt
     1081 ggggtgaaca atgtcctgta aaatcttgtt gtgaagataa aaaccttaca cattgtggtc
     1141 tatgtgatac atttccatgt gaaatgctta atcaatttgc atatgacaaa gaacaaggtg
     1201 atgatggcaa aagaatttct cagtgcaaaa aatgggctgt actagcctta taatatactc
     1261 tcttgttata aaataagttt ttgtaaggta aattttttaa actgttgtag aaatttctgc
     1321 aacagttttt ataatt
//
LOCUS       DAYATN010000275         1247 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.280, whole genome shotgun sequence.
ACCESSION   DAYATN010000275 DAYATN010000000
VERSION     DAYATN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.280"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..462
                     /locus_tag="ACGI8A_003770"
     CDS             <1..462
                     /locus_tag="ACGI8A_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFH0181687.1"
                     /translation="VLEVFDKLMEKMTEARVPENGRILYVTPVVNTLIKNAKEIQRTV
                     NIKDGGTSLNRQTTDIDTVKIIKVPSNLMKTAYDFTTGWKVGAGAKQIFMSLVHPSAI
                     ITPVSYQFSKLDEPTAVTEGKYFYFEESFEDVFILNKKADAIQFVVEGAGA"
     gene            462..695
                     /locus_tag="ACGI8A_003771"
     CDS             462..695
                     /locus_tag="ACGI8A_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181688.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            705..1106
                     /locus_tag="ACGI8A_003772"
     CDS             705..1106
                     /locus_tag="ACGI8A_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181689.1"
                     /translation="MENNMIDDIERRLQGFGYVLKDGDKWLIDFVREKIENIIKLDCN
                     ISEIPKELHNIEIDMIAGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTTYGKNNILTFRCLRW"
BASE COUNT          530 a          133 c          233 g          351 t
ORIGIN      
        1 gtattagaag tatttgataa gttaatggaa aaaatgacag aagctagagt acctgaaaat
       61 ggaagaatat tgtatgttac tccagtagta aatacactta tcaaaaatgc aaaagagata
      121 caaagaacag taaacataaa agatggtgga acttctttaa atagacaaac cacagatatt
      181 gatacagtta aaataattaa agttccatct aacctcatga aaactgcata tgattttaca
      241 actggatgga aagtaggagc aggagctaaa caaatcttta tgtccttagt tcacccaagt
      301 gcaataatta cacctgtttc ttatcagttc tctaagttag acgaaccaac agcagttaca
      361 gaaggaaaat acttctactt tgaagaaagt tttgaggatg tatttatatt aaataaaaaa
      421 gctgatgcaa tacaatttgt tgttgaagga gctggagcat aatggcacaa gtaagaaaat
      481 taaatagaat attaactata gaagaatgta aaatagatga tttcttagag atgggatatg
      541 atttgataga tgaaactggt aaggcagtaa agtatggcaa gtcattaaat gtaaaagatt
      601 taatagctga aaataatatt ttaaggtcaa aagtcgagtc tttagaagaa gaaaataagc
      661 agcttaaaga gaaaaataag cttactaaaa agtaggtgaa aattgtggaa aataatatga
      721 ttgatgatat agaaagaaga cttcaaggat ttggatatgt tctaaaggat ggagataaat
      781 ggttaataga ttttgtaaga gaaaaaatag aaaatattat taaactagat tgtaatataa
      841 gtgaaattcc aaaagagtta cacaatatag aaatagatat gatagctgga gagttcttat
      901 ttaccaagaa aaatatgggg caattagata tagaaagtat taactttgaa gctgtagaaa
      961 agtctatatc agaaggtgat acaaaggtag attttgctat aggaagtggc tctcaaacac
     1021 cagaacaacg ctttgatagc ttagtagctt atcttactac ttatggtaag aataatatat
     1081 taacctttag gtgcttaaga tggtaagtaa aactagaaaa gcaatagaaa tgttatatag
     1141 agataaatgt actatagttg agtatcagcc aattaaagac cctgtaacaa aacgaactaa
     1201 caataaagaa gtgattgtat tagaaaatca accatgtaaa ctttcat
//
LOCUS       DAYATN010000276         1188 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.281, whole genome shotgun sequence.
ACCESSION   DAYATN010000276 DAYATN010000000
VERSION     DAYATN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.281"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(348..839)
                     /locus_tag="ACGI8A_003773"
     CDS             complement(348..839)
                     /locus_tag="ACGI8A_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFH0181690.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          457 a          163 c          108 g          460 t
ORIGIN      
        1 tatgaagtta taatttaata tatatttatt ttatttgtga gtctattttt atatataatg
       61 aaaaatgtga taagtatcca aaagaattac tggatataat aggatagggt atttagcctt
      121 ttaagataaa taaaaatatc tacaactata tattgcaaaa atttaaaact aatttatata
      181 tgctaatata aatatttata aagacataag acaaatttac ctacagattt aaatttaact
      241 tatatatttt aaaaggctaa tataatatgt attttcttct atacaatcaa aaggcttcat
      301 atctaagata tgtgatgaca ctaatattca caatttttta gtaattatca cattataaaa
      361 aatattacag ctagtccatc atcagatgct tcttgtataa actttttaag ctcaattaga
      421 tttgcccata catattcaaa aaaatcatct tcactctcat cattcataac aggataaata
      481 ttattttcta acatctcatt aatattaaat ctttttctaa atgcttgttc ttccaaactg
      541 ttcattgctt ttgatagttt ttttacatca tttgctgtaa tcaacatgac aggaaattct
      601 ccatcaattt ccataagtat gtttccactt agtactaatt tgctaaatat attatcatca
      661 tctccaccaa aaggacaacc tgtcaaagta aagtgaatgg catgccatgc tttatcaata
      721 ttgatagtac tattttcgtt ggcatcttca aaaacaaaat cttgtaagtt ttcttctgct
      781 tgctgtaatt taaaaacatt ctcttcactt attttagtat aacatcctat catccccata
      841 attacattcc tcctaagtag cgttttttta attgtaactt ataattaaac ttagttacaa
      901 taaaatatat tgagaaaagt attatttcat cttaaattta aatacctata ttatcaacta
      961 aaattctgtg tttatagcgt aagtattatt attttttcct aaaaattaaa tgaatcagaa
     1021 gttctatagg caatattgac tataaataag aactattact tttaaaaaaa aatttaagaa
     1081 attatactgt agaatcatgc tctacttatt agctttaaat aaaaaataaa tcatgttctc
     1141 aaattaaata ataaataaca ataaatttaa attttaaact ataagagt
//
LOCUS       DAYATN010000277         1178 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.282, whole genome shotgun sequence.
ACCESSION   DAYATN010000277 DAYATN010000000
VERSION     DAYATN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.282"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            61..1101
                     /locus_tag="ACGI8A_003774"
     CDS             61..1101
                     /locus_tag="ACGI8A_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFH0181691.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLSLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          461 a          140 c          194 g          383 t
ORIGIN      
        1 tcagaacatt tgtatttatg ttataatttg tataaatata tttatttagg aggaaacaat
       61 atgaatttaa atttaactaa tactataaat ttaatgaaaa aatatttgga cattccaagc
      121 ccagctggat atactgaaaa tgcagtttta gaaatcaaaa aagattttga aaatttaagt
      181 cttgacacta ttcttacaaa aaaaggtgca cttatagcta ctcttcctgg tgaagatgat
      241 tcaaatcaaa taacaatatc tgcacatatg gatacacttg gagctatagt aaaagaaata
      301 tcatctgatg gtactcttaa ataccataaa gttggtggtg gatgttgggc ggctatagag
      361 ggagaaaact gtactgttat aactagaaaa ggtaaaaaaa tcagaggaag tgttgtattt
      421 aagtatgctt ctactcatat atatggtcaa acaaaagctg ccactgaaag aaatgaagaa
      481 acaatgatta ttagacttga tgaaaaagta tttacaaagc aagatgtatt agatttagga
      541 ataagtgttg gggattttgt atgcctagac catagatttg aggctacaga atctggtttt
      601 ataaaatcta gatatataga taataaatca gctgtagcaa tggttcttga aatatgtaga
      661 tattttaaag aaaacaattt aacacctaag catactacta acttttatat aagtaattat
      721 gaagaaattg gacatggtgt ttctaaatgt ttacctgaaa aaacaaaaga atttgtagca
      781 atagatatag cacctactgg accaagtcaa acatctagtg aacatggtgt aactatagtt
      841 gcaaaagata atttatcatt atatgatttt aatttaagaa acaagcttgt cgatgttgca
      901 gaagacaata atttagacta cgctgtcgat gtatttactt tctatggttc agatgcttca
      961 gaagctattc gttggggaga agatgttcaa atagcatgtg ttggtcctgg aacaaacaat
     1021 agtcaccatt atgaaagaac acatatagaa gctattgaaa atacactaaa attacttata
     1081 aattatatgc ttaaagaata attaattata tattttacca aagtcaattc ttaacaaata
     1141 attaacttta atttttattt ttatacaaaa attggttt
//
LOCUS       DAYATN010000278         1125 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.283, whole genome shotgun sequence.
ACCESSION   DAYATN010000278 DAYATN010000000
VERSION     DAYATN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.283"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            239..943
                     /gene="cwlD"
                     /locus_tag="ACGI8A_003775"
     CDS             239..943
                     /gene="cwlD"
                     /locus_tag="ACGI8A_003775"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HFH0181692.1"
                     /translation="MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTII
                     LDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEEN
                     NKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
                     KCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKECKLLTDETYQ
                     EKIAWAIYIGIQKYLS"
BASE COUNT          500 a          113 c          167 g          345 t
ORIGIN      
        1 taaattctga atatacgtta tcaaaattta taatataaac taagtataat ttattgtata
       61 gtattgaatg ttagataaac atagtgaatt aaatagggac agaaatataa tatcattaca
      121 taataatata ttaataccaa taaataaaga tatatcaata ttaaaaaaat ttaaatgttt
      181 gtactaatat atggaatatt atagtgtttt tataagtata catatacagg gtgatgttgt
      241 gagaaagtac ataaaacata taatttttag ttttgcaatg atatgtctag tagtagtatc
      301 catatttgaa attaaaaata tttctgaaga tgttatcaag tatatgccag taacaaacaa
      361 aactataatt ttagatgctg gtcatggagg cattgatcca ggtgcattaa ataaggataa
      421 gagtacatct gaaaaggata ttaacctagc aataacactt aagcttagag agcttataga
      481 atcaagtggt ggccttgtaa tattaactcg agaagatgac agtagccttt ataaagaaga
      541 aaataataaa actacaagac aaaaatataa tgagaatttg aaaaatagaa aagaaataat
      601 atctaattct aatgccaata tgtttgtctc tatacatctt aatgcatttg aacaatcaaa
      661 atactatgga gctcaaactt tttatcctaa agacaaacaa gacagtaaag aattatcgaa
      721 gtgtattcaa gaagaactta aaagagtagt agataagaca aacaacagag aagtcaaacc
      781 aagggatgat atatatcttt tgaaagaaaa taatattcca tcagtactga tagaatgtgg
      841 ttttttatca aatgaaaaag agtgtaaact cttaactgat gaaacatatc aagaaaaaat
      901 agcatgggca atctacatag gaatacaaaa atatttaagt tgaaataact ataaaaatag
      961 gtctaaatat gtaaatagct tgtattaaag cagttaaaat gcattaatat aggctatttt
     1021 tattttgaca aaaaaatatt taaaataaaa gttaaaaagt tgttgactta gaataatata
     1081 gatgatatta tatatgagtg cccaaaagga gcaccaaaat aagac
//
LOCUS       DAYATN010000279         1075 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.284, whole genome shotgun sequence.
ACCESSION   DAYATN010000279 DAYATN010000000
VERSION     DAYATN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.284"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            425..892
                     /locus_tag="ACGI8A_003776"
     CDS             425..892
                     /locus_tag="ACGI8A_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0181693.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          421 a           71 c          171 g          412 t
ORIGIN      
        1 agaaagatta tgaaaactaa attagaagaa ctaaaaacta tatttttatt aattataata
       61 tttgaataat ctttttaatc atataaaatg catatacttg taatatttaa aggtgatatt
      121 aatattatta gtgtatatta atattccctt gaaattaaaa aaacatagat atttcagtaa
      181 tctaaaatag tctacaaaaa taacagtttt agcaaaatta ataagaatac atatagattt
      241 aaacctaaaa gtatgaacat gtctgttttt tacaagatta aaatttatat tatatgataa
      301 aattaactta actatttcaa taagacttat aatttttttc tttatactag accttattga
      361 aacctttata gtttaattct aattatatat tataaataaa gtatgtgtat attaaggagg
      421 taatatgaag tttttaaatg ttttagtagt agttgaagat attgagaaat caaaaaagtt
      481 ttattatgat gttttggggt taaaggtaat ctgtgatttt ggagaaaatg ttgtattaga
      541 aggtaatata tcattacaag aaaaaaagct ttggttagaa tttattaata agagtgacag
      601 tgaagttaag ttcaatggaa atgatgctga gttatatttt gaggaagata attttgatac
      661 ttttgttgaa agattgagta caatgaaaga tatagattat gttcatctag ctatagagca
      721 taggtggggt caaagagcaa ttagatttta tgatttagat gggcatatta ttgaggttgg
      781 agaaactatg tcatctgtgt gtagacgttt tttagatagt ggtctttcaa tagatgaggt
      841 agctaaaaga atggatgtaa ctgtcgaata tattgaatca gttttagaat agatggatta
      901 tattaaagta aaattgaggt gaaggctgta agtaaacttg tgtgaaaaca aatttgctta
      961 cagtttttat ttttctaaaa taaagaatat aatacttgtt tagctttatt tgagttttta
     1021 aaaatattat gatatttctt atatatttat actaaagttt aaatttagaa aaaat
//
LOCUS       DAYATN010000280          998 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.285, whole genome shotgun sequence.
ACCESSION   DAYATN010000280 DAYATN010000000
VERSION     DAYATN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.285"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(57..767)
                     /locus_tag="ACGI8A_003777"
     CDS             complement(57..767)
                     /locus_tag="ACGI8A_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFH0181694.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          332 a          137 c          112 g          417 t
ORIGIN      
        1 taagctttaa aaagttaagt ttcaaaaata aaattctaga gattgaactt caaaaattaa
       61 gattctaaca tataccttgt aaattttcct acaacctctt gatatttttt acggcttata
      121 ggaatttttt cattctgaac tttggcataa gtttgagtta tactatctac atggtttaga
      181 ttgacaatat acctttgatg cgtacgtata aaattagagg gaagaacagt ttccatatca
      241 tttaattttc cgtagtattc taatgtttta tcaaaggtaa ctacagttaa ttttcttagc
      301 tcacttttaa agtagaggat atcttttgta ttaattttac aaagattagt tccaatctga
      361 actgtaataa agttttcttt ccattgttct aattttttta gtgcttcttt taatacttta
      421 catatttttt cagttttata aggttttaag atgtagttca aagctcctac atcatatcca
      481 tgaaatacat agtcagaaaa acctgtgaca aagatgatta tagtatcttt tgaatgtttt
      541 cgtatagttt ttgctatgtc aacgccatta ctttttccaa gttcaatatc taaaaaaata
      601 atatctatgt aataattttc gtgttctaga ttgtatacta gactttctcc atttgaatat
      661 tcttttattt ggtaggggta gccccacaaa tccatttctc ttgtaatgat tttattcagc
      721 atgtttcttt gttgtttttc atcatcacaa atacaaatcg aagtcaattt tttatctcct
      781 ttcaagtttt atatttatat agttaagtat ataatatatt ttgctaccta gatatataaa
      841 atgaaataac aattatatcc agatagcaaa ataaaatttt ttatattagt atacttacac
      901 ttaaaactat tctattaaac ttattctgaa gtgctaatga ttttcttatt aaaaaatcta
      961 acaatctttc ttattataaa atacaaatgc ttttatta
//
LOCUS       DAYATN010000281          977 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.286, whole genome shotgun sequence.
ACCESSION   DAYATN010000281 DAYATN010000000
VERSION     DAYATN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.286"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..675)
                     /locus_tag="ACGI8A_003778"
     CDS             complement(<1..675)
                     /locus_tag="ACGI8A_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HFH0181695.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGP"
BASE COUNT          314 a          157 c          103 g          403 t
ORIGIN      
        1 tggtcctatg cttcctgtcg ctcctgtact tccggttggg cctgttactc ccgttggtcc
       61 tgtacttcct gtcgctcctc ttggtcccat tggacctgta attctaattg gaagacactc
      121 acatttagga taacaattat ctataattct atcttcaaat gttacaagta tatacggttt
      181 gttacaacct ctactagatg taaattcaag aatctgataa gggtaattta aagataaagc
      241 taacccataa ttaggatatt tattcattgc ccatgctttt atagttcccg taatatttaa
      301 acaaatatag ttggatgtat cacttatccc aactggtaaa tatcccctaa tattttccat
      361 agacactcta tcattccatg ttatagttcg taaattataa tattcactta ttcttttaat
      421 ttcaatatct actttttctt taaaaatatg attcttattt gaatcaatat gaataaataa
      481 ctctgctttt aatatacttt tgtcttttaa tatatctggt attctcactt taaaaaaagt
      541 aatcaacttt tcttttacaa aactatttga acatttactt cctattaagc aataatctaa
      601 attactaaag tttttatctg gatatttttt agatataaaa gtatcatcat ttaaataaag
      661 tataatattt ctcatttata atccctccat tttgggtcta tatcatacta tgtttaaaat
      721 acatgcttag ttacaatata attaaaattt atagtattaa atttttatat aataatgctg
      781 atttctacaa aaaaagaaca tgtcattaga aatttctaat agcatgttct ttttcattat
      841 tatatattac actttaaatt attccttgac tggcttagct ggtgtaaact aaaccagtct
      901 ttctaaaata ttaagcactt ttttcttatt atgtttttta ggatttattg tctatattat
      961 tgtaaaccaa agttata
//
LOCUS       DAYATN010000282          846 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.287, whole genome shotgun sequence.
ACCESSION   DAYATN010000282 DAYATN010000000
VERSION     DAYATN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.287"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            247..753
                     /locus_tag="ACGI8A_003779"
     CDS             247..753
                     /locus_tag="ACGI8A_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HFH0181696.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          336 a           82 c          138 g          290 t
ORIGIN      
        1 tacatttttt tattgtatat tgctttaggt ttaaaaatat attatatact ttataatcaa
       61 tatattttat ctatatttta cacttataaa atatgaaaac tacttattgc gaaagtaatt
      121 gatttgttgt attataattt acatgaatta tgtaggacaa tcaaaagtca gtattataag
      181 agtacatata ttcaaatttt taacaattaa tttatataat taaaaaatga agggtaggat
      241 gaagtcatgg gttttagaga agtaaaaatt gaggaattac agtttaatcc atttactaag
      301 attggcaaag aatggttact tataacagct ggtgattcag aaaagtttaa cacaatgact
      361 gctagctggg gtggtgttgg agtctattgg ggtaaaaatg ttgttacaac ttacataaga
      421 ccacagagat atacaaaaga atttgtagat agtaatgata catttacagt tgccttcttt
      481 gatgaaacgt atagagaggc tttaaatatt tgtggtacta tttctggtag agatataaat
      541 aaaatagaaa aagcaggact tacaccatat tttgtggatg atacagtagc ttttgaagaa
      601 gctaatatga ttatagtatg taaaaagcta tatcatggta atatgccacc agaaaacttt
      661 gatgctaaag aaaatgataa aaaatggtat ccagagaaag actaccatac tatgtacatt
      721 tcagaaatta taaaagttct tgtaaaagaa taattataat aaaaaatata tgaaaagaac
      781 ttattataag taaatgactg tgctttctta tctgatatct aagtagatgc tagtaactaa
      841 ataaat
//
LOCUS       DAYATN010000283          787 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.288, whole genome shotgun sequence.
ACCESSION   DAYATN010000283 DAYATN010000000
VERSION     DAYATN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.288"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>787)
                     /locus_tag="ACGI8A_003780"
     rRNA            complement(<1..>787)
                     /locus_tag="ACGI8A_003780"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          238 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actgggg
//
LOCUS       DAYATN010000284          753 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.289, whole genome shotgun sequence.
ACCESSION   DAYATN010000284 DAYATN010000000
VERSION     DAYATN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.289"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..>753
                     /locus_tag="ACGI8A_003781"
     rRNA            <1..>753
                     /locus_tag="ACGI8A_003781"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAYATN010000285          695 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.290, whole genome shotgun sequence.
ACCESSION   DAYATN010000285 DAYATN010000000
VERSION     DAYATN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.290"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            101..>695
                     /locus_tag="ACGI8A_003782"
     CDS             101..>695
                     /locus_tag="ACGI8A_003782"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFH0181697.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFL"
BASE COUNT          290 a           83 c           89 g          233 t
ORIGIN      
        1 tagatagtta ctatatctta taattagata aatcataatt atatattatt gttaaaactc
       61 aattatataa tttttaataa tattatttta ggaggtaaaa atgataaacg atattaaaaa
      121 taaattcaaa aattacaaac catatataaa tggatacaag aatatgaaga gagcatctgt
      181 tttaattcca atagtagaaa taaataacac acactatata ttatttgaag ttcgctcaaa
      241 aaatttgaag catcaaccta gcgaaatttc ttttccaggt ggtaaaatag aatcaggtga
      301 gactccatat gaagctgtta ttagagaaac ttgtgaggaa cttggaactt ttagtgacaa
      361 tatagaagta atatcaagtc ttgacctcct tataacacca gtaaagttta tcatacatcc
      421 atacttagga tatataaaaa atattaataa tttaaatata aacaaggatg aagttgacca
      481 tacttttttg gttcctataa aatatctttt agagaatcct ccaaatactt atagtaatac
      541 tgtaaaagtt tttccagatg aaaatctacc atatggttca attccaaacc aaaaagatta
      601 taaatttgaa agtggaagtt atcctattct gttttataaa tataaagatt atgtaatttg
      661 gggaatgaca gcaaaaatac tagaaaactt tttaa
//
LOCUS       DAYATN010000286          577 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.291, whole genome shotgun sequence.
ACCESSION   DAYATN010000286 DAYATN010000000
VERSION     DAYATN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.291"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..>577
                     /locus_tag="ACGI8A_003783"
     rRNA            <1..>577
                     /locus_tag="ACGI8A_003783"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAYATN010000287          530 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.292, whole genome shotgun sequence.
ACCESSION   DAYATN010000287 DAYATN010000000
VERSION     DAYATN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.292"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="ACGI8A_003784"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="ACGI8A_003784"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="ACGI8A_003785"
     tRNA            310..398
                     /locus_tag="ACGI8A_003785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
     gene            410..486
                     /locus_tag="ACGI8A_003786"
     tRNA            410..486
                     /locus_tag="ACGI8A_003786"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:cat)
BASE COUNT          173 a           93 c          114 g          150 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt ccctgatggc
      481 ccaccacaat aatttaaaga actttgaaaa ttaaacagta ggttaattta
//
LOCUS       DAYATN010000288          496 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.293, whole genome shotgun sequence.
ACCESSION   DAYATN010000288 DAYATN010000000
VERSION     DAYATN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.293"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..>496
                     /locus_tag="ACGI8A_003787"
     CDS             <1..>496
                     /locus_tag="ACGI8A_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFH0181698.1"
                     /translation="GTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLD
                     QAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFD
                     GYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRT
                     VASGV"
BASE COUNT          213 a           61 c          125 g           97 t
ORIGIN      
        1 ggaacagttg caacaggaag agtggaaaga ggagtactaa aagtacaaga cgaagtagaa
       61 ttagtaggat taacagaagc accaagaaaa gtagtagtaa caggagtaga gatgttcaga
      121 aaattattag accaagcaca agcaggggat aatataggag cattattaag aggagtacaa
      181 agaaacgaga tagaaagagg acaagtacta gcaaagactg gatcagtaaa ggcacacaca
      241 aagtttacag cagaagtata tgtacttaaa aaagaagaag gtggaagaca tacaccattc
      301 tttgatggat atagaccaca attctatttc agaacaacag acgtaacagg agcttgtaag
      361 ttaccagaag gaatagagat ggtaatgcct ggagataacg taacaatgga agtagactta
      421 ataaactcaa tagttgtaga agagggatta agattctcaa taagagaagg tggaagaaca
      481 gtagcttcag gagttg
//
LOCUS       DAYATN010000289          484 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.294, whole genome shotgun sequence.
ACCESSION   DAYATN010000289 DAYATN010000000
VERSION     DAYATN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.294"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>484)
                     /locus_tag="ACGI8A_003788"
     rRNA            complement(<1..>484)
                     /locus_tag="ACGI8A_003788"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          115 a          143 c          108 g          118 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 acca
//
LOCUS       DAYATN010000290          416 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.296, whole genome shotgun sequence.
ACCESSION   DAYATN010000290 DAYATN010000000
VERSION     DAYATN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.296"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     regulatory      146..236
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          179 a           44 c           50 g          143 t
ORIGIN      
        1 attttcaaag agtttattat aaaattgtaa actatagtgt ttataatctt tgtaattatt
       61 ggtaattaaa tttaaaaaat aaaatattta attatacaca aaaaagatat ttaaaaaatt
      121 tgttatttat tgtatactta agttaaaact aaataggcaa atctagagaa atctagtgac
      181 gcaaagctat agggactaag gtttatatac ataaactatg tcagccagtt gccaaaaaga
      241 gtcctaggtg tattgtatac ctaagaaaag tctataatga ctgccttttt ggcagtcatt
      301 ttgttatgga aaaatcactt tatttcagta gaaaatacac ataataacat attttaccaa
      361 ttataacata aaataaagaa aaattatctc aaaaagttac tattttacaa aaaaga
//
LOCUS       DAYATN010000291          379 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.297, whole genome shotgun sequence.
ACCESSION   DAYATN010000291 DAYATN010000000
VERSION     DAYATN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.297"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
BASE COUNT          157 a           32 c           70 g          120 t
ORIGIN      
        1 atataattaa aatttttatt gtagttattt agcaaataat gaatacttat aatatataag
       61 gttgaaaaaa cagtatattc attgacaaat ataagtttta agtatatatt aaaaattatt
      121 agagtgtgca ttagaatgta tacagcgtat attaagaatt attagagtgt atataaggat
      181 gtatacagtg tatattaaaa attattaagg ggagggggag tttatgcaac aaaaatgtaa
      241 aaagaaagca ctatcaatga ttttaagtat ctcaatggga cttggaccag tagcatcatt
      301 taatgcgtca aatactttcg cagatactat agaagaaagt actaatgcag gaaatgcaga
      361 aggaggtact aatgcagga
//
LOCUS       DAYATN010000292          364 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.298, whole genome shotgun sequence.
ACCESSION   DAYATN010000292 DAYATN010000000
VERSION     DAYATN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.298"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="ACGI8A_003789"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="ACGI8A_003789"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="ACGI8A_003790"
     tRNA            291..362
                     /locus_tag="ACGI8A_003790"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAYATN010000293          356 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.denovo.299, whole genome shotgun sequence.
ACCESSION   DAYATN010000293 DAYATN010000000
VERSION     DAYATN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.denovo.299"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..353
                     /locus_tag="ACGI8A_003791"
     CDS             <1..353
                     /locus_tag="ACGI8A_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFH0181699.1"
                     /translation="IKGVEKDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIE
                     VKLVKENYTSQTCPKCSKKHKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFR
                     INHKQPINLKVSTV"
BASE COUNT          159 a           39 c           62 g           96 t
ORIGIN      
        1 atataaaagg ggttgaaaaa gatactaaga aaaagaaaag attaaacaga gttaatagac
       61 agaaaatatc tcaaatggag tatggtcgaa taaaggacta tataaaatat aaagcaaaag
      121 agcaaggtat tgaagttaag ttagttaaag aaaactacac ttctcaaact tgcccaaagt
      181 gttctaaaaa acacaaacca actggtagaa cttatagttg tacttgtggt tatgaaactc
      241 atagagatat agttggtgct tggaatattt taaataaaaa gcataaatat ggtttagtag
      301 attttagaat aaatcataag caaccaataa atttaaaagt atctaccgtt tgagcc
//
LOCUS       DAYATN010000294         9130 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.guided.141, whole genome shotgun sequence.
ACCESSION   DAYATN010000294 DAYATN010000000
VERSION     DAYATN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.guided.141"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            263..955
                     /locus_tag="ACGI8A_003792"
     CDS             263..955
                     /locus_tag="ACGI8A_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0181700.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            952..1887
                     /locus_tag="ACGI8A_003793"
     CDS             952..1887
                     /locus_tag="ACGI8A_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0181701.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            2035..2958
                     /locus_tag="ACGI8A_003794"
     CDS             2035..2958
                     /locus_tag="ACGI8A_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181702.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            2942..3712
                     /locus_tag="ACGI8A_003795"
     CDS             2942..3712
                     /locus_tag="ACGI8A_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181703.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            3722..4504
                     /locus_tag="ACGI8A_003796"
     CDS             3722..4504
                     /locus_tag="ACGI8A_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181704.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            4881..5417
                     /gene="rbr"
                     /locus_tag="ACGI8A_003797"
     CDS             4881..5417
                     /gene="rbr"
                     /locus_tag="ACGI8A_003797"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HFH0181705.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            5449..5838
                     /locus_tag="ACGI8A_003798"
     CDS             5449..5838
                     /locus_tag="ACGI8A_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HFH0181706.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            5875..6261
                     /locus_tag="ACGI8A_003799"
     CDS             5875..6261
                     /locus_tag="ACGI8A_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HFH0181707.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            6388..7830
                     /locus_tag="ACGI8A_003800"
     CDS             6388..7830
                     /locus_tag="ACGI8A_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HFH0181708.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            8127..8942
                     /locus_tag="ACGI8A_003801"
     CDS             8127..8942
                     /locus_tag="ACGI8A_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0181709.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         3204 a         1172 c         1760 g         2994 t
ORIGIN      
        1 aatgtattat catgtttgtt ttattcaagc acacttataa tacgcttgat tttttcacaa
       61 tgatgaaata tactaatggg tgttataggt ataagaatcg cattacactt gaagaagtaa
      121 gtgataaatg aggtataaat cctcatatga taaactacta tttcattgag gaacatattc
      181 taagtgatgt caagatacta caatatgact aaattcctag tgataaaaaa gtctacgaat
      241 gagtgtagaa aggggaacta agatgaataa agttttaatt atagatgatg ataaagatct
      301 ttgtattttg ttaaaaaaga gtatttcaat tgaacagatt gaagcaaact attgccattc
      361 tgggagagat ggactggttg aactttcaaa acaagattat cagttagtag ttctagatgt
      421 gatgatgcca ggtatggatg gttttgaaac tatggaacaa atacgaaaat atagcagtat
      481 tcccatctta atgctgacat caaaaagcga taattacagc aaagtgcatg gattgcgagc
      541 tggtgcagat gactatctaa ccaaaccatt tgaaattaca gaatttactg ctcgtgtact
      601 gtctttaatc cgacgttaca cccgttttaa tccaaaaaat gaggagccac agcaactttc
      661 tttcaaaggt ttgttgattg accttgacca gcatacagtt actaccaatg gcgaacctat
      721 gttgcttcca cccaaggaat ttggtgtact tctttactgt gcaaggcatc agggtaagat
      781 tttgacaaaa cagcagattt atgaagccgt gtggggtgag ccatatgtat atgatgacag
      841 caatatcatg gctattatca gtcgcctaag aaaaaaaatt gaactagacc catcatctcc
      901 catctatttg caaacaataa aaggaattgg gtatcaattc aacacggagg tatgaaaatg
      961 gatgaacaaa cgataattct tttcttactc ttgctgtcga ttggtctact ttttctttcc
     1021 atatttgctt tttccaagct tgggcatatt tacaagcgcc taaaagatat agaggagata
     1081 ctggctgatg tacaaattgg agacgaaaat cgtaaaatac taattaagcc atgtgatgtt
     1141 atggctccat tggtttatca gcttaatgag attgtctacg attatgaaaa taagttactt
     1201 tctctgaaaa agtcggataa agctagcaaa caacttatga caagtttatc tcatgatgtt
     1261 cgtacaccat taacaactct gattggttat ctggatgctg tacacagtgg tatagttatc
     1321 gaacaagagc gggaggaata ccttgaaatc gctaggcgta gagcctacga cctcaaggat
     1381 tacattgatg ttctttttga ctggtttcgg ttgaactctg atgaatttac gctgtcaata
     1441 gaatctgtgg aaattgcaga gttgtcacgc aatattttga aagactggat tcctatattc
     1501 catgaaaaga aactgaattt tgaaattgat attccagaaa atcgactaat ggtaaacctc
     1561 gacccagatg gatattcccg tgtggtcaat aatctggttc aaaatgttct tgctcacagt
     1621 aaggctcggc aaatcaagat taccatgagt gaggattcca gaatggtatt actccgtgtt
     1681 gaggatgatg gtgttgggat tgccagagaa aatttaccac atatttttga gcgactgtat
     1741 aagtgtgata aaggacgttc agaaaaagga agtggtcttg gtctatctat tgtcagtcaa
     1801 atggtggagc gaatgggtgg tcaggtttcc gtagaaagtg agattggaaa gtataccgta
     1861 tttacagtga gcttaccatt aatataaaat tgtttttccc ccatgtgaca tgtatacaca
     1921 tggggatttt ttgtaagtta taaagttgca aggttaatac aaggttgttg caaggttaat
     1981 gtaagattga ggtggtatga taactttaat aaacaaagga ggcttttatt atttatgaat
     2041 aatttaatta ttgaaacaaa aaatctcact aagcaatatg gtgctcaaaa gagcgtttct
     2101 aatttgaata ttcacgttca gaaaggtcgc atttacggat tgctaggaag aaacggtgcg
     2161 ggtaagacga caactatgaa aatgttattg ggtctaacaa aaccaactac tggagaaatt
     2221 aaaatatttg gtcaggacat tcacaataac gagaagaagc ttcttcctcg aattggatgt
     2281 ttgattgaat caccagggtt ttatcctaat ctgacaggaa cagaaaatct caaaatcttt
     2341 gcaaggctta gaggaatacc aaaaaaaaat gcagtagaat cagcactgga tgtagtcggt
     2401 ttgccaaaca atgacaaaaa gttattttct caatactctc ttggtatgaa acagaggttg
     2461 gcgattgctc ttgccattat gcatgaccca caactgttga ttttggacga gcctatcaat
     2521 ggacttgatc ctattggtat tgcagaagta agaacgttta ttcgtgatct ctgcacagaa
     2581 cgaggtaaaa ctattttgat ttccagtcac attctttctg aaattgcctt gttggcagat
     2641 gacattggca ttattgacca tggtattttg ttggaggaag aaagtcttgc ccaattagaa
     2701 gaaaaaaaca gtaaatacat acattttgct gtatctgatg cagcacaggc agcaaggctt
     2761 ctggaaaccc aacttaacct gaaaaatttc aaagtagcag ataattacaa tattatgcta
     2821 tatgatacag atgtttctgt tgcagaaata aatcgtgctt tcattctaaa caacattgag
     2881 gtatcagaat ctcatttatg cgaagatact cttgaagatt acttcaagaa agtaacaggg
     2941 ggtgaaggaa ttgcttgata taatttcatg tgaattttca aaactcaagc ggttaaaatt
     3001 tatactgatt tctattttag gtgcttgctt atttccaatt cccgcaacaa ttttgattgc
     3061 aaaggacaac ttgccctttg agcaattgtt caaattagtt gtcaattttg gatatttttt
     3121 gctattacca attgtattaa gtattgttgc tagccaacta ttttttatag aaagagataa
     3181 tgatgtatta aaaaatcttg caacagtgcc agtgcaaaaa ggaaaactgg ctctggcaaa
     3241 attggctgtg ttgctgttta tatcattgtt ctactctgtt gcagggttag gagctactat
     3301 cattggaggt ttcattgttg gaatagtaga gggggttgct gtaaaattag gaatgagcat
     3361 tgcttttgga attatgtttt ttgttactgt acttcctgtt gtggtactca ttgtatattt
     3421 caatcgcagc tatatttttt ctattatact ttcttttgta tatgctattt tcaattttag
     3481 tatatcctta aatattatca attttgaacc aaataaccca atattaagtg tattacctgc
     3541 tcctgttata atgcgttggt ggatggcatt ttggggagat cccactggcg aatacacggc
     3601 acttagacag ccatatttgc tctcaactcc tgcttgtgta ggtattttat gtttgataac
     3661 cattatagca gtcctgctga tttgtatgat atacaaaaag caggagaatt aggaggtatc
     3721 tatgcttgat ataattacta cagaatttca aaaaataaaa agatataata ttctatggat
     3781 tggtgttgtt gctgttctgt tttcagcact attggcagtt tttcaggttg taagttcaca
     3841 cggcagtgac cctctaacgt atgagagctt tgcaaacgga gttatctgga ataatttcag
     3901 tcttagtttt ccatttggga ttacacttat tggaggatat cttattaacc gtgaatatac
     3961 agaccaaacc ttaaaaaaca tcctaactgt tcctatttca ttacgcaaat tgctagtggg
     4021 aaaattaatt gcggttggag gaattactgt attgttaagt cttttcagct tcttatgcac
     4081 atttattttg gggttgttgt tttgcaatat caatatatct gttacactcg ttgtaaaatc
     4141 cttagttcag attttgtctg ttaatctgta ttgttatgtt gctgttttgc cgattattgc
     4201 ttattttggt cgtaaacaaa atgcatttct gacgggtgta gggattgcat ttgtatacgg
     4261 tttttgtggt gtgtttgttg ctggaagaaa tctgacagac ttttatccaa tcacagcagg
     4321 tcttggtcta gttggataca acaatggtgc tggtactgtt tatcagccac ttatcggtgc
     4381 tatgactttg attgtaattc taatcttgac aactattcta cttgtattta cacctaatta
     4441 tgataaagtt atgaccatat ctaagaaaaa agggataaag cataaaaagt attctcataa
     4501 ataacataaa tattgtttct attatttaag ttgctagaca gtgaaaagaa aaaatctgtc
     4561 gtacagtgtc tatatgctca tgtattcaag aagctacagt tttgattttt cataactgta
     4621 gcttcttttt gtatttatta aatcacgact atcataatac ttgcaatagg tatagcgaca
     4681 agagagagta ctgtcatatg tgttttatat attacattaa gtatattcta aaatatatgt
     4741 tttaaataat atatttgaaa ataggaatat ttaaataaaa aaaataatat tatatatagt
     4801 tgacaaaaat tggcaaatga gataatatat aaatatagaa atagtaatca ttactattat
     4861 taaaaaggga ggaattaatt atgaacttaa aaggaactaa aacagaaaag aatttaatgg
     4921 caggatttgc aggagagtca gaagcaagaa ataaatatac atactacgct tcaaaagcaa
     4981 gaaaagaagg ttataatcaa atagctgcta tatttgaaga aactgctaac aatgaaaagg
     5041 aacatgctaa attatggttt aaacttttac atgatggtat gccttcaaca gaggaaaatt
     5101 taaaggatgc agcagcaggt gaaaactatg aatggactga tatgtatgca aaatttgcta
     5161 aagaagctag agaagaagga tttgacaaga tagcttattt atttgaagct gttggaaaaa
     5221 tagagaaaga gcatgaagaa agatacttaa agcttttaga aaacttaaat gaaggaaaaa
     5281 tatttaaaag agatgaagaa gttgtttggc aatgtcaaaa ctgtggtcat gtgtatgtag
     5341 gtactgaagc accagaaaaa tgcccagtat gtgaccatcc aaaagcgtat ttcaatataa
     5401 aagctgaaaa ttattaaaaa ttatcattat taaaaaggaa tgttaactat gaaattttct
     5461 aaacaacgag aactgatttt aaatgaaata ttaaataatc cggttcatcc tactgcggat
     5521 tacctatatg aaaacttaaa aaaagataat ccaaatttaa gcttaggaac tgtgtataga
     5581 aatttagctc agctaacgga acatggcttt ataagaaagg ttagtattcc agggtatcca
     5641 gatagatttg atggtcgaat agataatcac tatcatatca tatgtgaagt atgtggagag
     5701 gtatacgatt tagaatccga agttcttaat aatttacaag aattaatatc tgaggagaca
     5761 gacataaaaa taacatctta taacataagt tttaaaggga tttgtaataa ttgtaaaagg
     5821 tgtagccaag taggttagtt ttcaaattct aaaaataatt aatgaggtga gtttatgtgt
     5881 agtgaacaaa aattttttat atgtgagact tgtggaaacc ttgtaggaat gatacaaagt
     5941 ggtggtgtac ctattttctg ttgtggaaaa cctatgaagg aacttgttcc taatacaaca
     6001 gatgcagcag ttgaaaaaca tgtacctgta attgaagtgg atggaaataa tgttactgtt
     6061 aaagtaagta gcacaactca tccaatgact aaagaacatc atatagcatg ggtgtatctt
     6121 atgactgaac aaggtggtca acgcaaatgt ctagcagtag atggagaacc tgtagttaaa
     6181 tttgctttga atgatgatga taaagtgatt tctgcctatg cttactgtaa tttacatgga
     6241 ttatggaagg ctgaactgta atatttaaat aaattgagtc aaaaaggagg ctataaatgt
     6301 attttcatac aaatagcctc ctataaattt atataaattc aagttttaat tttgtttaat
     6361 gtcaatataa tataaggaga aaataatatg tcaatttata aatgtagtgt ttgtggatat
     6421 atttatgatg aatctaagaa tgataaaaca tgggatgaat taagtgaaga ttgggaatgc
     6481 cctgtatgta caaaaggtcg tagttatttt ggaaagataa gtactgttta ctatgaagaa
     6541 gatgaaaaga tagcagaaga catagttgaa gatgaatcta aattaaatac tgaaaaagag
     6601 ggagatttaa attacttaag tacatattta agaagagatg atgaagttga gaaacatatg
     6661 gatataattc acgaaatggc tgttacagga aaatctataa tagaacctat gagaactaaa
     6721 ctaccagtaa tttcttggga tgatatttta attatgggtg ctcaattaaa tccattacct
     6781 ttaaatgagc atgatgaagt aaatacaaca acaataatag gaaaaaaagc aaagaaacct
     6841 atgattatag aaaatccagt ttatatttcc cacatgtcat ttggagcttt gtctaaagaa
     6901 ctaaaaatag ctttagcaaa gggtgcagcc caaaataaga cagctatgtg tagtggtgaa
     6961 ggaggtatat taccagaaga gaaggaagct tcatacaagt atatttttga gtatgtacca
     7021 aataaatata gtgtaactga agaaaatctt aaaaattctg atgctataga gataaaaatt
     7081 ggtcaaggaa caaaacctgg tatgggaggt catcttcctg gcgaaaaagt aactgaagaa
     7141 atagcaaaag taagaaataa gcctgtaggt caagatgtaa taagtccatc ttgttttgag
     7201 gaaatacaat ccaaagaaga tttaaagaaa cttgtagatg aacttagaga agtttcagaa
     7261 gggcgtccaa taggtgttaa aatttcagca ggtcatattg aaaaagatat ggaatttata
     7321 gcttatgcta aacctgattt tgtaactatt gatggtcgtg gtggagctac tggagcaagt
     7381 ccaaagttgc ttaaagatgc aacttcaatt ccaacaatat ttgctcttta tcgtgctaga
     7441 aaatacatag atacacatgg attagatata gatttggtta taacaggtgg gcttcgtata
     7501 tctactgatt ttgctaaggc aattgctatg ggtgctgatg cagttgctat tgcaagttct
     7561 gctcttatgg cagcggcgtg tcaacaatat cgtatatgtg gttctggtaa gtgtcctgta
     7621 ggagtagcta ctcaggatga ggaattgaga aaaagattac atatagaaaa cagtgctaat
     7681 agagtggcaa actttttaaa tgtaagttta gaagaactta agacttttgc tagaatttct
     7741 ggtcataaag atatacatga tttaagtgtt gatgatttat atactgttaa ctctgaaata
     7801 tcaaattata caaacataca gcatgtatag attgaagttt gaaactatta gattttagat
     7861 agctataaaa gtaaatgata aatatatttt ataaaagaga gtgtcttaaa atgaactttt
     7921 tagttcataa gtcactcttt tttttatgaa gccaaatata tttatcattt tagcaatgaa
     7981 aaagaatctt atctctttat tttgagatag actccttcta tttatgaaaa ttaaagatat
     8041 taaattgtaa aatattctgt gatgtcgtac tattaaacta aagattctgt actttaaagt
     8101 aaataaaaat ttaaggagtg attattatgg aaagattgaa gatacaagtt ttagttgaca
     8161 ataatactta tattgataga tactttgttg gagaaccagc agtaagctat tatattgaga
     8221 ttgatggaaa taggattttg tttgactcag ggtattcaga tgtattcata tcaaacgctg
     8281 aaaaattaaa tatagattta ggaaatttaa ctcatgttgt tttttctcat gggcataatg
     8341 accatacaag aggtatccag tttctagaaa ataggtatga tttatctaca gttgaactta
     8401 tttcacatcc taactgtttt atacctagaa aacatggaga gaaaagcata ggagctccat
     8461 tttctgctga ggaaattaaa aatattttta tgtataatcc taaggataag ccatttaatc
     8521 taagtaagaa ttgtgtgttt ttaggtgaaa taccttctat aaatgatttt gaaaaaagag
     8581 ctaaaattgg taaatgtaaa ataggagatt tgtgggaaga tgattatgtt ttagatgatt
     8641 ctgctatagt ttgtaaaact gataaaggta tttttattgt tacaggatgt tctcatagtg
     8701 gaatttgtaa tatagtagag tatgctaaaa aggtatgtgg agatgataga gttattggta
     8761 tactaggtgg ttttcatctt tttgaattga ataataggtt agatagtaca atacaatatt
     8821 tggataaaga agaaatgaga atgttgtacc cttgtcactg tgtttctttt aaggtaaaag
     8881 cgaagatgaa tgaaatattg aatataaatg aagttggagt agggctgacc ataagtatat
     8941 agtatttgaa agtatataag atttaagttg aaggttaaaa agatattttc tatagaaaat
     9001 atcttttttg ttacgtttat tttaagagaa atcaatatac ttttatatga aatattgtaa
     9061 aaaaatttat ttttaatgag aatatatttt cattaataaa caattttatt atgtattaat
     9121 aatgttagaa
//
LOCUS       DAYATN010000295         7756 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.guided.161, whole genome shotgun sequence.
ACCESSION   DAYATN010000295 DAYATN010000000
VERSION     DAYATN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.guided.161"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(172..1008)
                     /locus_tag="ACGI8A_003802"
                     /pseudo
     CDS             complement(172..1008)
                     /locus_tag="ACGI8A_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1079..1747
                     /locus_tag="ACGI8A_003803"
                     /pseudo
     CDS             1079..1747
                     /locus_tag="ACGI8A_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1874..2494
                     /locus_tag="ACGI8A_003804"
     CDS             1874..2494
                     /locus_tag="ACGI8A_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFH0181710.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            2650..3432
                     /locus_tag="ACGI8A_003805"
     CDS             2650..3432
                     /locus_tag="ACGI8A_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFH0181711.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            3557..4483
                     /locus_tag="ACGI8A_003806"
     CDS             3557..4483
                     /locus_tag="ACGI8A_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0181712.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            4497..4946
                     /locus_tag="ACGI8A_003807"
                     /pseudo
     CDS             4497..4946
                     /locus_tag="ACGI8A_003807"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            5306..5713
                     /locus_tag="ACGI8A_003808"
     CDS             5306..5713
                     /locus_tag="ACGI8A_003808"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0181713.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            5715..5945
                     /locus_tag="ACGI8A_003809"
     CDS             5715..5945
                     /locus_tag="ACGI8A_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0181714.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            6335..6538
                     /locus_tag="ACGI8A_003810"
     CDS             6335..6538
                     /locus_tag="ACGI8A_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFH0181715.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            6616..6948
                     /locus_tag="ACGI8A_003811"
                     /pseudo
     CDS             6616..6948
                     /locus_tag="ACGI8A_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            7041..7673
                     /locus_tag="ACGI8A_003812"
     CDS             7041..7673
                     /locus_tag="ACGI8A_003812"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFH0181716.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         2747 a         1137 c         1357 g         2515 t
ORIGIN      
        1 attaaatttg attatctttt cataagttgg taaatgtcga tatatcctgt atctacaagg
       61 agtatcggca ttttcttttt ggaagatact ttgcataagt tggcatttaa ccgtataaga
      121 atgccatcaa aagtagtaaa taagtagtaa actctagtca tactactagg attataaccg
      181 ttccatttca gctctcgaag aactaaatga accatgtgca taatagccaa gcgtcatagt
      241 aatgttttta tgccccataa tatattgcaa tatattagga ctcattcctt tgttagccat
      301 atttgtacag agtgtatgcc taaaggtatg tggcgtaatc tttggtagct tgtcctcatg
      361 ggtcttatta tacttcttga ctaacgctct taacatgctt tgataatgag ttgcttgttt
      421 tggtaatcca tcttgtttaa gaaataggaa gttctggtaa ccatcaacaa tgattggttc
      481 tgctttagcg ttcttacgcc gttccacgat acgttcaaat gattgataag ctctttcagt
      541 cattggaatt tctcgcttac cctgtgtggt ctttggaact tctacataat agccaatctt
      601 agtatcactc aacaattgat ggtctaccat aatggcttta tttttcatat ccaattctaa
      661 agtcaaaccg cataattcag aaatgcgtag tcctgtttct aagaggataa caacatcatc
      721 ataatattta caataagtct tatcgctccc cataaaggat aataagccca tttcctgttc
      781 tggtgttaga gcgatttttg gttctgttgt atcttccaaa acatcactta actaaaaatt
      841 aaatggattc tttctaatac agtcgtcttg aattgccatg aaaaacgacg ctttcagtga
      901 gcgcttataa ttgtcaatgg atttataagc acacccatct tcactcattc ggatagacca
      961 gcttttagcg tctgataatt taaccatatc aatgcttttt gcacctagtg ggtcattgat
     1021 agtatctata ttttgattta taatttatac atttttttaa aaacagactt ttaactgtaa
     1081 agaatctatg tataaactta gctatgttgt ccgtgtatct acaaagaatc ttgacgagct
     1141 aaaaaggcgt tgtgatgaag tcaaagactt ttacgacgac ttatcaatca agctcgtgcg
     1201 tccctttggg gatatgttgg gattgcatgg cgaatttatt cctgtcagca aacgttacat
     1261 caatgattat attcaatatg tcacttcgga ttttttggct ggacttggtt ttggagcgac
     1321 acaaaccctt ggcgaacatg aggatatcta tcttggctac aatctggaca ctggaaagaa
     1381 tgtctatcta aaacctgccc ttgctagtca aggggtaaag ggttctatta ccaatgcgtt
     1441 agcgtctgcg tttatcggtt cattgggtgg tggtaaatta ttcagtaaca atatgcttgt
     1501 ttactatgcg gtactattcg gcggacaggc ggtcatagtt gaccctaaag cagaacgcgg
     1561 caaatggaaa gaaacgctac cagaaatagc ccatgagata aacattgtca atttaaccag
     1621 taatgatgaa aacaaaggat tgcttgaccc ttacgtcatt ttgagccgta aaaaggattc
     1681 tgaaagttta gcgattgata tcctaacatt ccttactggt atctccagtc gtgacagtga
     1741 taagtttttg cagaaaaatg acaactggaa aataataatg gaatagtttc tatgtaaata
     1801 tatttttact atcttaccta actaattgaa agatgatata atttaatcaa agatttggta
     1861 aggagggatg aatatgcatt taattgattt tatgaatgaa gatattaaaa agaagcttgt
     1921 taaaaaaacg tataaaaaga aagagactat tctttttgct gagcaagaaa atgaatatgt
     1981 aattcttatg attgacggta ttgcagaagc attcatctta aatgtgaaag gaaacatctc
     2041 aacgattcat ctttatagaa gtggtagttt ttttggagaa atggaacaat ttaatgatgg
     2101 gaaaaaaccc gttgaaattg tcgctttcac agattgtaat gtatataaac ttcatagaga
     2161 tgatttttta aactggttaa ggggagattt tgaagctaca aaatttctga ttcgagaaat
     2221 atctagtaaa ttagttgtaa atgctgaatt aattgaagaa ttatcatctt taagtgttaa
     2281 agaacgactt ctgaggtgta tatccttaca ctattataga agtgaattaa atctgcttac
     2341 caaaaaacag cttgctagcg aagtaaacgc tccaattaga agcattaatc gtgctataaa
     2401 tgaatgtgta caagaacatc taattgctta tgaaaacaaa agatttagtg taattaatca
     2461 gcgagaggta ttaaagaatc ttcctacaac ataagtataa tttcaaaaaa gtattgaata
     2521 ataattattc aatgcttttt tatttcgggt caagtgacct attttttttt atttcttcat
     2581 tatataatac tcctatgcat aattatgttg gattgatagc taataaaaga cagtgagagg
     2641 tgttatatta tgactaaaac acaacagaaa atgatacaaa acgtattaga attttcaaaa
     2701 agagatagta ggataatagg acttgcaatt agcagttctt atatcacaaa atcgttagat
     2761 gaatattctg atttggattt tctgattgta gcagatgata aatcctatga agaagttatc
     2821 gaagagagat tacttattgt ggaacagttt ggaacattag tttctgcttt tactggcgaa
     2881 cacgttggag aacctcgatt gataatttcc ctctatgatt cccctgttct tcatgttgat
     2941 tttaagttta caaccttaga tggtttacta gatagggttg aagattcagc tattctctat
     3001 gaagaaaaca actgcataac agaaatttat tctaaaaaag aagcatactt cccaactcca
     3061 gacttacagt ggattgaaga tagattttgg atttgggttc attacgcttg cactaagatt
     3121 ggacgtagag aactatttga aacaattgat tttttatctt ttttaagaca atcagtactt
     3181 gccccattaa tacagcttct cttaggtaaa ttacctcgtg gagttagaaa aattgaattg
     3241 gacgcacccc aatatattga agcattagaa aagacggttg ctaaccatga atataaaagc
     3301 tgtgttaatg cattgcaggc tactatcaac ctgtatcttg agttaagaga ggaattatca
     3361 caaagtccta ttgtccgaaa ggttgaagct gaaagggttg cattacagta ttttcaagat
     3421 ttaatcaaat aaacccaaag taaaaaaata ttgaatagta aatattcagt gttttttcat
     3481 ttttggtcaa atgacctaga ttagttgttg taacaattct ataattattt tgaaagtaac
     3541 agtatggagg ataactatga gtgatatttt aagtagaatt acaaataaaa ttggggataa
     3601 gaatatagtt gataagttat cagctctatc aaaatcagat ttgaactcac ttctattaga
     3661 agtgtttgat agacaagcaa atactttaac tgcaactgat attctaaagt cataccaact
     3721 aaatagattt actatcccta gcggtataga tccagaagaa attcatgctt tggaatcaaa
     3781 attattgaga aaagcattca atatggatat caagacgatt atgctttctc cctctgcccc
     3841 attaggtagc tgtagcgttt ttggctccgt agatcaatat aacgtagtaa gtgctctacg
     3901 agggacagaa acacttgcag acccctcaaa tatgttggca ataattattg ctgataaatt
     3961 aaaaagaaaa gaagaaaata ataccatacc tatccacatg gctactactg ctagggtaat
     4021 aagagcacaa aattttgtag gtaaaggttt atattcacat tttggcttat attgtatggt
     4081 ttcatctggt atagatacag ggtcttacac ttgtgaaaaa atgttgttag aaaagcactt
     4141 aaactattat aaagatatat taagtgaaat agaaaatgtt cgtttctcta ttattctacg
     4201 caaaagaaac ggatacaaag ataatgacgg atttttcgac agaatggttg aaactatcaa
     4261 acttattttt cctaatatag agttatcaat tcataatgat gatgttgata ataattatta
     4321 taaaggtatc aatttcaaat tatcggtcaa acaaggaaat gaaaccgttg aaatagttga
     4381 cggtggattt gttgactgga cttatcaaat gcttggaaat aaaagagaac gctgtttgat
     4441 aagtggtatt ggcttagaaa gatttcttgt agctgtttca taaaaggaga agaaaaatgg
     4501 aacttataga gtggaattat gcttatatta ccgatttagt tgagtttgcg aatgataaag
     4561 aaattgcaaa gaatcttcgt gacaacttcc cccaccctta tagggagcaa aacgctagag
     4621 attttgttag tttttgtttg gcaacttcag atgttaaaca acttagctat gctatttttt
     4681 atcaagaaag ggctattgga agtatcagcc taacatttga agaagatacc tctattaata
     4741 aaaatgctga attaggttac tggcttgcta ggaactattg aggcaaaggg atagcaacaa
     4801 atgcagtaaa agaaatatgc aaaattgcct ttgaaaaata caaccttaac aaaattcatg
     4861 ctaatgtata ttcctacaat agagagtcaa gtaaagtatt ggaaaaatgt ggctttaaga
     4921 ttgaagaata ctgtcacaat tcataataaa gaatagaaac atgattgatt gccatgtata
     4981 tagtttaatt cgaccgcaat agcatttatt agaattaacc tactttttaa taacagaata
     5041 agtttttccc ctctattaat ttcttatgca tctttgtatt tttatgaaaa gctagccaat
     5101 gcatatttcc atacatcaat atggtggtgg cttacaacca atataaatta aatacgtttt
     5161 aaagaataca catttttgcc ctataggcag agtgtgtatt tttttctctt ttttttgaaa
     5221 tttatcgaag atttaacgcc ctcccaccgt gataggagta agcaaggtta tttaactcct
     5281 tgcgatggaa agagggtgaa atcttatggt tgatgaaaag acaattgaac atcaattcga
     5341 cagtttctgt aaaacagttt tgcgtaacta tgctagagat atttatgacg aaaataaacg
     5401 cagaaatgat tatttggtat ctctcgaatc tttgtcactt gcagaattaa gtaaattgag
     5461 tattttagat gattatgatt ctaactacat ctgtatggtt tcttacgact acaacatacg
     5521 aattgaagat gtattaatgg cgcaagcaat cggaaaactt acaaaaagaa aacaagatat
     5581 aattctactt tcattctttt tcaacatgac aaatgctgac atagcaacat tgatggattt
     5641 agcagaaaat accatacatt atcacaaaac aaatgcacta aaagagttga aagaactcat
     5701 gcaggaacat tgaaatgaaa cctactaaag aagtattacc ttttacaatt attttacttg
     5761 ctgtacatgg cgatgtaaat gcaataaacg taatacttaa acattttgaa cactatatca
     5821 ttcgtctttc acagaaaact ttatttgatg aatacggaaa tccctatatt catgtagaag
     5881 aagaaacaaa gcgaatgcta ggaacaaaac taatcactgc tattttgaga tttaatttgt
     5941 cttaatctcg ccctctctcc atgtatcaag ctatttggta catggagaat cactaattat
     6001 ttgtctataa aagcccacaa gctattgtga ttttttatag gcaaatcaat gccgactgtt
     6061 ctttgacaac tgaataaagt agatagatac gtttgtgata cagatagcca acaaatacta
     6121 tacgccatga ccttaatcaa gagagctact atatagtgat ttgtgaataa cctttggaac
     6181 ggttgttttg ccgtaacctg taccacagta taatgataca cctgtatgac acggttctgt
     6241 ccataagaat gggattggtt gaaataccaa tggagcttat caaagccatc tatctacttc
     6301 tgaactacga acatagaaag gagtgataca ttatatgaac aatactgatg tacctatttg
     6361 ggaaaaatat accttgacga ttgaagaagc gtcaaaatac tttcgtattg gagagaaaaa
     6421 attacgtaaa cgtgctgatg aaaaccttga ttctggctgg gtcattatga atagcaatcg
     6481 cattcaaata aagagaaaac aatttgagaa aatcgttgat acacttgacg ttatctaatg
     6541 aaattgagcc ttatatgtta taatattcat atgacgtatc aagctcaatt cggttattga
     6601 gaggagcttc ttatcatgtc aactgaaaaa agacgagata ataaagatag aattttaaga
     6661 actggagaga gccagagaaa agatggaaga tacgcatata agtatgtata ttcatttggt
     6721 aaaccacaat ttgtttactc ttggaaactt gtacctactg ataaaccccc aaagggaaaa
     6781 cgtgatgata tatcattaag ggataaggaa aaagcaatac aaaatgacct tgatgatagc
     6841 attgacacta ttggtaagaa aatgacagtt tgtcagttat acgctaaaaa gaatggacta
     6901 agaaagaata tccgacttaa cactaaaaaa ggacgccatt atcttatgaa gattcttaac
     6961 aaggagtgat tctgtgaaga gatgtattgt agtagtggaa tagtcaaaaa cataatatat
     7021 attaaaggag agatttaaat atgactattc caagttcaaa taaggtatat ccaagaagta
     7081 atgattatca aactgtatat cttaaaaatg taataacaag ggaaaatata gaagttggag
     7141 attacacaat atataatgac ttttataatg acccaagaga ttttgagaaa aacaatgtat
     7201 tgtatcatta ccctataaac aatgataaat taataattgg taagttttgc tcaatagcat
     7261 gtaatgcaaa atttctaatg actagtggaa atcactcaat gaaatcaaaa tccacatata
     7321 cttttcccat cttttatgaa gaatggaaac tcgatatcaa ccatataacg gacgcttggg
     7381 acaataaggg taacattata attggaaatg atgtgtggat aggttatgat tctataatta
     7441 tgtcaggtgt aaatattggt gatggtgcaa ttattggaac aagagcagta gttactaatg
     7501 acgttccacc gtattctatt gttggaggag tacctgcaaa aattataaaa aagagattta
     7561 ataatgatac aatttcaaaa ttattagaaa ttaaatggtg ggattggcca tataaaaaaa
     7621 ttcaatcaaa cattaagcat attcaatcag gaaatattga taaattagtt taatatttta
     7681 taatttattt atattaaact ttactttaat aatcatttta taagagcttt ttcaaaattt
     7741 gtatattatt ttaata
//
LOCUS       DAYATN010000296         7505 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.guided.165, whole genome shotgun sequence.
ACCESSION   DAYATN010000296 DAYATN010000000
VERSION     DAYATN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.guided.165"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            328..1029
                     /locus_tag="ACGI8A_003813"
     CDS             328..1029
                     /locus_tag="ACGI8A_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HFH0181717.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            1079..2161
                     /gene="vanS"
                     /locus_tag="ACGI8A_003814"
     CDS             1079..2161
                     /gene="vanS"
                     /locus_tag="ACGI8A_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFH0181718.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            2422..3522
                     /gene="vanG"
                     /locus_tag="ACGI8A_003815"
     CDS             2422..3522
                     /gene="vanG"
                     /locus_tag="ACGI8A_003815"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HFH0181719.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            3519..4325
                     /locus_tag="ACGI8A_003816"
     CDS             3519..4325
                     /locus_tag="ACGI8A_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFH0181720.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            4343..6481
                     /gene="vanT"
                     /locus_tag="ACGI8A_003817"
     CDS             4343..6481
                     /gene="vanT"
                     /locus_tag="ACGI8A_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HFH0181721.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            6795..7415
                     /locus_tag="ACGI8A_003818"
     CDS             6795..7415
                     /locus_tag="ACGI8A_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFH0181722.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
BASE COUNT         2744 a          832 c         1301 g         2628 t
ORIGIN      
        1 ttttagtaat gtctgtggtt acatacatag gtttaattgt tttttatatg gattgtctct
       61 ggtataataa attgaattgt gtttgactga tagatatttt tattgaaagt taatacctgt
      121 tatgcgataa taaggacgtg ttaggggagt ttttataata acaattaatc tcagtttttg
      181 tgttccaata gtagcatcaa cacattctga tgtagtagtt cttttcttat aaaaattagt
      241 tacaaatatg cttgtaatta tttgatatgt aaatttacca atattataaa tgatgacctc
      301 gataataagg agagtgaaaa ataatctatg aacgaaaata ttttgattgt ggatgatgaa
      361 aaagaaatag cagatttaat tgaagtatat ttaaaaaatg aaggttttac agtatacaag
      421 ttttatacag gtaaggaagc tctagaatgt attgaatcta aaaatttgga tatggctatt
      481 ttagatatta tgcttcctga tattgatgga tttcatattt gccagaaaat tcgagaaaaa
      541 tactattatc ctatcattat gttaacagct aaaattgaag atatagacaa gataatgggt
      601 ttaaccatag gtgctgatga ttatataaca aagccattta accctttgga agttgttgca
      661 agagtaaaga cacaattaag aagatatatg cgatataata attcttatga acaacagtcg
      721 ataattgtta atgagtatga tattaaagga ttgattatca ataaagaaac acataaatgt
      781 agtttatttg ggaaagaagt agcattaaca ccgattgaat tttctattct ttggtattta
      841 tgtgaacatc aaggaaaagt ggttccttct gaagaattgt ttgaagctgt atggggagaa
      901 aaatatcttg ataataataa cacagttatg gctcatatag ggcgattacg tgaaaaaatt
      961 catgaacctt ctaaaaaccc gaaatttata aaaactgtat ggggagtagg ttatacaatt
     1021 gaaaagtaaa aatattaaag tcaatcctga ctttactcaa gtgagaaata aaatttttat
     1081 gagaacagct ttaatggcat ttatagccat tattttcata tggattttat attcttttat
     1141 attgtatggt aagttttcta tctggatagt atcattcttt gaaaatgctt ttcgtatgga
     1201 atataaagct gcattgaatt tatatcagca gattttccgt aatagcatgg agctcttcat
     1261 gatactagct atggtgattg tgttttttat aatattccgg gtttatttaa attggtttac
     1321 aaaatatttt agtgaaataa atcaaggtat tgactctctt atcaaagaag atgttggaga
     1381 agtggcttta tctccagagt tattggcaat agaaaagaaa ataaactcaa ttaaacatat
     1441 acttgaacaa cgtaaatttg agacacaaat ggcagaacaa cgtaagaatg aacttattgt
     1501 ttatcttgca catgatttaa aaacaccact tacatcagtt atagggtatc tgacactact
     1561 tcgagatgaa agtcagattt ctgaagaact gcgaaaaaaa tatttatcta tttcgttgga
     1621 taaggcagag cgtcttgaag atttaattaa tgaattcttt gaaattacaa ggtttaattt
     1681 atcaaatata tcacttgaat acagtacagt aagcttaact cgtatgcttg aacaactaac
     1741 atatgagttt aaacctactt tgatgagtaa gaatttaaaa tatacactga atgttacacc
     1801 aaatatgatg ataaggtgtg atgttaataa aatgcaacgt gtatttgata acttattacg
     1861 aaatgctgtt tattatagtt ttgaagatac tactattgaa ataacagctg tacaggagga
     1921 tgattatata aagcttaaat ttataaatca tagcaatacg attccagagg aaaaacttga
     1981 gcgtattttt gaacaatttt acaggttgga tacatctcgt aattcaaaca gtggtagagc
     2041 tgggttggga cttgctatag ctaaagaaat agtggagctt cataacggga caataacagc
     2101 tcatagcgag aacaatataa ttatctttaa ggttattatt ccgattgtag gaaatttgta
     2161 agaatttaag tataaaaaaa taggaaattc tttaggataa ataaataaag agcactcttg
     2221 ttattgatat aattactgta tcaaaacaag agtgctttta ttttgataca gtaattatat
     2281 caatagaaaa atgtgaaaat atattatgtg gaacagatgc aattgtaagt gaaggttgat
     2341 tggatttgaa atagaaaaag tattttaaca tgtttctgta gattgtataa aaataaataa
     2401 atcaacaaag gagaatcaaa gatgaataat aaaaaaattg cagttatatt tggtggaaac
     2461 tcaacagaat atgatgtatc attacaatca gcttttgcag tacttgagaa tttgaatacg
     2521 gaaaaatatg atattatacc aattggaatt acaagggagg gtaattggta tcattatatt
     2581 ggagattata ataatatcca gaataacact tggtttgaaa gggatactga tataattcca
     2641 gttattgttt cgcagaaccg tgtcaaaaat ggtattattg aatttttaat tcctgatgct
     2701 gtttctcaga atcatattcc aaatagtgct attgaatcat gtgaaaatga tattaaatac
     2761 actaaacttg attttgtgtt tcctgtactg catggaaaaa atggagaaga tggaacagtg
     2821 caggggttat ttgaattggc aggtatacag gttataggat gtggtactct ttcttcagca
     2881 ttatgtatgg acaaagacaa atcacataaa cttgttcatt cagaaggtat taaagtacca
     2941 aaatcaatat tactaaaata ctcagataaa tttaatgtgg ctgaactgtc aaaagactta
     3001 acttatccat tatttataaa acctgtgcgt gcaggttcat catttggtat tagtaaaatt
     3061 tataaaaaag aagagttaca agtagcagta gacttggctt ttgaacatga taatgaagta
     3121 ataattgagg aaaatattga tggttttgag gtagggtgtg cagttttagg aaacgatgac
     3181 ttacttgttg gaagagtaga tgaaatagaa ttgtcaaaag gattttttga ttatacagaa
     3241 aaatatacat tgaaaagttc aaaaattcat atgccagcta gaattgattt ggattgtgag
     3301 aaaagaatac aagagacagc aaaaattatt tatagaactc ttggatgttc tggatttgct
     3361 agagtagata tgtttttaac tccatctggt gaaattattt ttaatgaagt caatacaatt
     3421 cctggcttta cttctcatag tcgttatcca aatatgatga aaggtattgg attgtctttt
     3481 gaagaaatat tggacaagat aattgaggtg tacataaaat gatgaataat attttattag
     3541 ataaaaataa agaagataaa atgacaaata ctatcttatt gactaaagat aaagtttatg
     3601 atgggaatct aattctagta aatgcttttt taccagttaa aacatctgaa gatatagatt
     3661 taattcctgt agatacaagg tttccaagta ttcttatgaa acgtgaagct acaaacattt
     3721 tacaaaatat tttaaaaagt atatgtggaa taaatgaaat agtacctgta agtggatatc
     3781 gtactgcaga ggaacaacaa gatatatatt caagttctct gcgtgacaat ggaaaagact
     3841 ttactaaaaa gtttgtagca cttccaaatc acagtgaaca tcagacaggg cttgctattg
     3901 atttagggtt aaagaaagaa gttatagatt ttatctgccc tgaatttcct tataatggta
     3961 tttgtaatga gtttcgtaag gcagcactac attatggttt tatagagcgt tatcagtgta
     4021 aaaaagaaga aataacagga atatctcaag aaccatggca ttttcgttat gtgggatatc
     4081 cgcattctga gattatcaat aaaatgaatc tcgctcttga agaatatata gaatatatta
     4141 agagattttc gtatacaaat tgtttaaaaa tagaagataa agaaaaaatt atagaaatct
     4201 tttatgtgcc tattcatact aaagaagaag tgagaatctc tatacctgaa caatgtgtat
     4261 atcaaatatc gggtaacaat aaggatggtt ttattgtgac attttggaga aatagaaagg
     4321 actaagaaat ggaggaacaa gaatgattag gaataaatct tatgcagggc ttgattattt
     4381 taggattata gctgcattac ttataattgc aattcataca tctccattat tatcatatag
     4441 taaaatggct gactttgtgt taactagtat aattgctcgt attgctgtgc cattcttttt
     4501 tatgctatca ggcttttttc taatttcaac atataattat aattgtaata aactgaaatc
     4561 ttttctaaaa aatgtattat ttatttacgg gatagctatt gtaatttata ttcccttaaa
     4621 tatttacact ggatattttt ccatgaataa tttagtgccc aatattatta aggacattgt
     4681 atttgatggg actttatatc atttatggta tctacctgca tcaattattg gtgcaagtat
     4741 tgcatacttt cttgtaaaaa gactaggttt aaagtactca ctagtaatca ctattgttat
     4801 ttatgtaatt ggtatgtttg gtgatagtta ttatggtttt ttagaaaaaa taccactatt
     4861 aaagaattta tatgggtata tttttgaagt atcagattat acacgaaatg gtatattttt
     4921 tgcacctgta tttttcatac ttggtggaat aatatctaat aaatctaatt atatgtcaac
     4981 taaaaatagc ttgattggat ttcttatatc attcttactt atgttatgtg agggaatgtt
     5041 tttacataaa ctaggggtac agcgtcatga tagcatgtat attatgttat tgccatgtat
     5101 gtatttctta tttacagcct taacattttt gaaaggttct cgagctaggt tattgcgaac
     5161 atcatcatta attatttata ttatacatcc tatgatgatt gttgttattc gtatgttttc
     5221 taaaatattc ggaatacaaa ctgtcttggt tgacaatagc cttattcatt atatagtggt
     5281 aagtatagca tcttttatag cttctataat tattatattg attcttaaac ctctaaaaaa
     5341 agacttgaaa ggagaacata aggtaaatac caatagagct tggattgaaa ttgatttaaa
     5401 taatttggaa cataatgtaa agatgataca aaaagctatg ccagaagatt gtgaattgat
     5461 ggcagttgta aaagctaatg cctatggtca tagtgctttt gaagtagcga cttgtgcaaa
     5521 ccaaataggt gtaaaagcat ttgcagtagc aacaatagat gaggggattg atttacgtcg
     5581 atatggaata ctaggagaaa tattgattct tggatatact aacccaataa gggcaaagga
     5641 acttcataaa tacaatctta cacagagtgt tattgacctt aatcatgcta tctctttaaa
     5701 caaacagaaa tacaacataa aatcacacat taaaattgat acaggtatgc acagactggg
     5761 ttttgatgtt aatgatgtga ggtcaatttt aaaggcattt aaattaaaat acttagatat
     5821 atgtggcata tttacacact tgtgtgtttc agatagtctg aaagatgaag atgtagactt
     5881 tacaaataaa caaattgaat gtttttataa gttaattgat tttctgttaa aaaaaggtat
     5941 aaaaattcct aaagtacata ttcaaagtag ttatggtttt tttaattatc ccaatcttaa
     6001 gtgtaattat gtaagaattg gaattgcatt atacgggata ttaagttctc caaatgatac
     6061 gactaaattg caacttgatt tacgacctgt tttatcctta aaatcacaag taattcttat
     6121 tagaaaaata caaaaagatg atagttttgg atatggaaga gtctttattg caagcagaga
     6181 tagcgtaatt gctatattat ctattggcta tgccgatggt atacctagaa atttatcctg
     6241 tggaaaaagt tacgtaataa taaatggttg tagagcagca atagtgggtc gaatttgcat
     6301 ggaccaactt gctgttgata ttacagatat acctaatgta gaagttggta atacggcaat
     6361 aataatagga agagataatt tatctgaatt atctgcttct gaagttgcaa ataactcatg
     6421 tagtatatcg aatgaattat tgagcagagt aggaagaagg ttaaatgtta taaagaagta
     6481 actcaattaa atagtaatat ttgttgtact gtgtaattta ttttactctg actagacatt
     6541 tttagtaata ttatgtctaa tatatatttt ttataactta catgacaatt taagttataa
     6601 aaaggtgatg tttcagattt ttattttgaa acatcacctt ttttgttata atatataata
     6661 aaataattat accttaactg taaacttata tagattattt actttttaat attagattca
     6721 ttttttgaga ttggtatata ataaacatat agagtatata ataatagtta aagttaaaaa
     6781 aggggaaaag caatatgcag tatatacaag aactacttga tttgactcaa aattattggg
     6841 tgttagccac aataatcggc ttattaagtg catttataga aagctttata ccagccttac
     6901 cacttgttgc tatagttaca gcaaatgctg caatacaagg attatttatt ggatgtttgt
     6961 tatcatggat tggctcagga cttgggacaa cttcattatt cttattaata agcagattta
     7021 atgatagtaa gctttttaat aagttgagaa atagtaaaac tgaaaaagca ataagttgga
     7081 tggataaaca gggatttaag ttattattta tagcgtatgc ctgtccattt atgcccggtt
     7141 gtttagttac tatcgcatca gcttttggta aaaaagattt aaaagatttt gtgccagcaa
     7201 tgttggcagg aaaatttgta atgtttatag ttataagtta cgtggcaagt gatatagaag
     7261 gttttataac aagtccttta aagatagcat catttatatt attggtgttt ttgtcatgga
     7321 agattggaaa tagagtaaat aaaaatcttg aaaatcataa ttatgatttc catcataaaa
     7381 aacatcacaa tgataaagat gataaaatga tttagcttgt acgaaataaa acgtatctta
     7441 aaatgattta gtactaaatt attttaagat acgttttatt ttttgtatta aggtacaaaa
     7501 ttttt
//
LOCUS       DAYATN010000297         6732 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.guided.181, whole genome shotgun sequence.
ACCESSION   DAYATN010000297 DAYATN010000000
VERSION     DAYATN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.guided.181"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(171..1943)
                     /locus_tag="ACGI8A_003819"
     CDS             complement(171..1943)
                     /locus_tag="ACGI8A_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HFH0181723.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(1957..2886)
                     /locus_tag="ACGI8A_003820"
     CDS             complement(1957..2886)
                     /locus_tag="ACGI8A_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0181724.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            3107..3292
                     /locus_tag="ACGI8A_003821"
     CDS             3107..3292
                     /locus_tag="ACGI8A_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFH0181725.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            3285..3986
                     /locus_tag="ACGI8A_003822"
     CDS             3285..3986
                     /locus_tag="ACGI8A_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0181726.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            3988..4911
                     /locus_tag="ACGI8A_003823"
     CDS             3988..4911
                     /locus_tag="ACGI8A_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0181727.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            5068..5991
                     /locus_tag="ACGI8A_003824"
     CDS             5068..5991
                     /locus_tag="ACGI8A_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0181728.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            5984..6721
                     /locus_tag="ACGI8A_003825"
     CDS             5984..6721
                     /locus_tag="ACGI8A_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0181729.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
BASE COUNT         2181 a         1135 c          957 g         2459 t
ORIGIN      
        1 attaatcttg cttaaagaac ctataatatt atatatttaa tttttaaatt attcttaaat
       61 cattttctat ataaatcatt gtataccttt tctgtgaaaa tttctacatt ttttataaat
      121 tttattcatt tttatatttt tatgtatata aattcttttt tgtaagttta ttaatttcta
      181 attatcttct ttaaatacat tgaaactaca tcagaaaatc tagttatatc tcttgaatat
      241 ataaaatcat ttccatatat taatctttca gcttcaagtt ctgattcttt tcctgtaaat
      301 actcctaata ctaatatatt ttgctttcta gcttttctca cttcattggc tgtatctctc
      361 aaaccaacta tacccctata agccttttct cctcttaatg ttctctcact actttttcct
      421 atctttacat catttggtct tccatcgctg agaactatca ggattttatt ttcttcatct
      481 ctatttatta gaccactaca tatagatttt attgccaaac catctctatt attaccagca
      541 caaaaatatt caaatatatt ttcactattt tttatactat catcataatc tttaaatctt
      601 tttaatattg tatagtctaa aaaactagaa aatcccatca ctctatttgg tatgccaact
      661 tctgttagag catttgcaat tatgtaagct tgaattgcca catttccctg atttctactt
      721 tgagagccac ttgcatccaa taatatatca ataacgtatt tccctttttc attttctata
      781 tctttataaa atattttatt attattactt ctaccaactc tccaagctct attggctaat
      841 atagtaccac aatcagaata tactctactt ttagcatttt cttctattaa aattcttgac
      901 agactttctt ttaatctctt tatttgctgt ctatacattc tagcatgttc tctaaatttt
      961 cctaaattat tttctttttg tcttgttaca tattttagtt gaaatacatt attacactca
     1021 cttctaagaa ctccatctgt aaagtgtatt ctacaacctt catgaacatt tctacaattt
     1081 tgtgtctcaa tttttttaat ttcaccttta ctcaaataag tttttccata ataatgttct
     1141 atttttgaat atatcttatt tgcagtgtct tcatcaacat atataactcg gtctgctgaa
     1201 ttgctacatt tagaatcatc aaaatcccct acaccttcta caagcatgga tgaagatatt
     1261 gaatctatat cactttcaat aatattttct tcttcatcac tataaagttc ttcatacatg
     1321 aaatcagcaa atgtatcaaa atttacttct acactttcta cattattttc atcaataaga
     1381 ctattttctt ctccatttaa tatgtattca aaataagtta aatatataac atctaatgct
     1441 tttaaaatct ccataatatc ttgattggta tctatatttc ttatatcatt aactattctt
     1501 ctagtcatac tatctaatgc tgggtgcttg tccatactat aaagtacaaa tgtgtattta
     1561 attttatcca taatattatc attgtgtatt ttggtaaaac gtgaaacaat atctttataa
     1621 gcattccttc ttatgtcttc tatccctggc ctgtctttaa tgaccttttc ttcaactatt
     1681 gtatttaata taacttgaat tagacctagt ataacatctt tgttgtatcc ttttcttatt
     1741 ctatttgtaa gatatttttt aactatatcc caatctacat actttcttct agcacctgca
     1801 attattgcga aatatagacc tatttctttt gaagagtaat ctttttctga tatatctata
     1861 ctttcattgt aatcaccagc tattgtccat gataggttat tagctctatt ttcaaattca
     1921 taatcatcat atatccattt cataatctca cctatattaa atatgactta ttggaaacac
     1981 atctatactc tcccatttat taggtattct agttttaatt acatctccaa ccatttcttt
     2041 ctcatataca tcaaaagtct ttcctgtaag tcccatatta attgcaagag ttggttttaa
     2101 tcctcttctt atagtcttaa gagatgccat aagaccacgt aaatcaatag ctttactaga
     2161 tatctctcca ttttgagact tcaattgtaa atctaaaaat attcctgcaa agtgtattaa
     2221 tttttcttca tcagcatctg aaaattcatt ttttaaaatc atcattaact tatcttcttc
     2281 tataggagga atatctatta ccataaatct agataccagt gcttcattaa gttcttttgt
     2341 tcctgcatat tcataattca tagtcccaat aaatcttgtt gctggatgta gattgactct
     2401 ctcataccca ggtacatcaa ttattcttct atagtctaaa gctgaatgta ccacaactat
     2461 agcatcattt tttgccatat taatttcatc aaacacccca aaacctccat taatagcaca
     2521 ctcataaact gagcctcttc taagtttaac ttcattgtct ataaatgtat ctgtacctat
     2581 aagtgttgaa ctgtctgtat ttatatgaaa tgatgtattc cactgaggtc tattaaatat
     2641 ttcccctaga ttatcagcaa gtaagttttt tcctgttgcc tttggtcccg ataataatat
     2701 attttcttct tctaaaatag ctgctataca catagacaag atatctttac cataaaaata
     2761 agttttagac tttgttactc tgtattctaa atctttttct aaaccataat aatttctaaa
     2821 atacagtaca tcttcaataa gtttttcatc taccccttgc acccttagat tatctattat
     2881 atccatattt ttaccccttt tatataaata gtatttgttt catttcttat caccatttta
     2941 gcataaaagg atataagcta aaattgattt tttaatctcc ccattttaag taaaaccatg
     3001 caccatttta tcactacaaa attaagagta ctacttactg atatttcttg attttgttcg
     3061 tctatacgta tataatatat gctgataaat tatgaggaaa ggatgattga aatatggatg
     3121 cccagaagta gcaaaaaaat ggggtatttc tgaaagaaga gtacaaaaac tatgtgaaga
     3181 aagtcgcata ccaggagttt caaaactcgg gtatatgtgg ctgataccaa agaacgcaga
     3241 aaagcctgtt gatggtagaa caaaaagaag aaaggaagta caatatgaat agcgttttga
     3301 ttatagatga tgacaaagaa ctttgtgctc ttatgaaaaa atgtgttgag caagaaaatt
     3361 tatctgctat tactgcacat tgtggtgcag aaggtctaca tttggtcgat aaaaatagac
     3421 acacactctc tcttgtaatt ttggacataa tgatgccaga tatagacggt tttcaagttc
     3481 tgaaaaaaat tagagaaaca agtaatattc cagtaattat gcttactgct aaaaatgacg
     3541 aggatgataa agtttccgga ctacgtttag gtgctgacga ttacctaaca aaaccgttca
     3601 gcataaacga acttatggct cgtgtcaatt ctcttattcg tcggtatact atgttaaacc
     3661 ctgcttttat aacagacaca gattgcataa taataaatgg catgattatt gacaagacaa
     3721 accgaattgt ttctgtcaat aatattcctg ttcaacttac aagcaaagaa tttgatttac
     3781 ttgcttttct ggcttctaat aagggacggg tgtttaccaa aaaacagatt tatacacaag
     3841 tatgggaaga agaatatgct tttgacgata gtaacatcat gtctttcatc agtaaattga
     3901 gaaaaaagat tgaaccagac cccaaccatc cattttatat tttgacagtt cgtggtgtcg
     3961 gttatcgttt taataaggag gcttaacatg aatacaagtg tctatctgct tttgatattg
     4021 gttcttagcc tgattattat agcgtacctt gtttctgtcc tgctacgtgt ccgaatacaa
     4081 cttacactta taaaagatgc tttagaggat ataaaaaatg gaaaccttaa tcgacgtatt
     4141 ttaacagatg aaaacgacat tacaaaacaa atttgctatg atattaacga aatagcaatc
     4201 aatagccaat ttcaacttat tcagcaaaaa caatcagaac aagcctacaa acagctaatg
     4261 acaagtctct cccatgatgt aaaaactcca cttgcttctt tggtcggtta tttggaagca
     4321 gtagaaagta aaattgttgt aggagaggaa aaagacgagt atatccatgt tgcgtctaat
     4381 aaggctcatt acttaaagaa ttttgtagaa aatctatttg aatgggtaaa attggattct
     4441 aaggaacaag tttttcattt tgatattttt gacttgaatg aattatcccg taatattata
     4501 tctgattgga tacctgtttt agaaagcagt cattttgaat atgaatttga tataccagaa
     4561 atagagtact tcttacgtat agacgcaaat gcatataccc gtatcattaa taacctatta
     4621 caaaatatta ttacccatag tagtggcaac aaaatgacat tgagaatttt tgaaaacaaa
     4681 gagcaagctc aaattataat aacagataat gggaaaggta tatcttcaga caatctccca
     4741 catatttttg aaaggttgta ccaatgcgac cattcacgag cttcaaaggg aaatggttta
     4801 ggcttggcga ttgcaaagga acttattaac gctcataagg gaaatattac cgccaacagc
     4861 actcccggta tgggaacaga atttacaata ttgcttccta aagccctgta aaagtacgag
     4921 gctttcttac tctaaaacaa gataaaagca agattatggc aaggttttgg caaggttcat
     4981 gttttataat tctaattgta gttgattttg acgttgtatc aactatcttt tgtagaaaca
     5041 aatttattaa aagtgaggaa attatatatg aatgattttg tgattgaaac aaagcaactt
     5101 acaaaaattt atgggaatca aacagtagtt gacaaagtca atctccatgc aaaaaaaggc
     5161 cgaatatatg gtctgttagg acgcaacgga gctgggaaaa ccacaattat gaaaatgatt
     5221 ttaggattga cttcaatcac gtatggcgag gtagatgtat ttggagagaa tattaaggga
     5281 cgtgaaaaac aagtatatcc ccgtatcggt gcaattatcg aaacgccagg attttatcct
     5341 aatctgacag gaacggagaa cttggagatt tttgcaaaac tgcgtggtac agccgcccct
     5401 aattctgtaa aaagagcatt ggaaattgta ggcttacctt ataaaaataa aaagctgttt
     5461 agtgaatatt cacttggcat gaaacagcga cttggtattg ccaacgctat cctgcatgac
     5521 ccagaactgt tagttttaga tgagccaacc aatggtcttg accctattgg tattgcggag
     5581 atgagaaagt ttattaggga cttgagcgtt gagcatggaa agacaattct tatttccagt
     5641 catattcttt cagaaatctc attgttggct gatgatattg gaattatcga ccacggtgtt
     5701 cttttggaag aaagtagtat gagtgaacta gaaaagaaaa acagtaaata tatcctgcta
     5761 caagtttcag atattccgaa gacatcactt attttagagc gtcaatttaa cacgaaggat
     5821 tattccgttc aagatgacta cacgttacac ctttataaca ctaatctgga tatgggtgaa
     5881 atcaataaag cacttgtaat gcagaatgta actgttatta gttctcaact ttgtaatgat
     5941 actttagagg actactttaa aaaaatcaca ggaggagagg gcattgctta aacttattca
     6001 aacagaattt cttaaactgc gtcgtaggaa acttatttgg ctgatgttgt tgtctgctct
     6061 cattatgccg tttttcgcac tactatactt caattatttt ggtaaaacag gtgttgagcc
     6121 aataaagttt tataaatggt ctgcatttgg ttatacactt tggattattt tgcctgttgt
     6181 tttgggtata ttttgcacca tgcttataca cgaagaaaac cagtatgata tgctcaaaca
     6241 gctttggatt gtacctatta gtaaaatggg atactttttt agtaaatttt tcgttgtttt
     6301 gatttactct atttgtttta tgctgattac agccgttgct tctgtgttat ttagtgtact
     6361 ttctggatat gtcgtattta gctgggcaag cattttatat ttgttgaaaa aatgtttaga
     6421 aattggtgta atcactgctt ttgtaatgtt gccgattttg gcaatcgcta catctcaaaa
     6481 aggatacatt ctcccaattt gtatcacatt ggtatatgcc ttttccggtt ttgttataat
     6541 gacaataaat atgtatttac acccattatc cagtatggga gttattatca tgagaaataa
     6601 agacattcca ggtcttatat tttcacagga aattaatgtc ccattagcat ttctttgtat
     6661 ttgtatttgg agtattgtat cagtattgtt tgcaaacact gcactaggaa gaagaaagtg
     6721 aggtgtagat aa
//
LOCUS       DAYATN010000298         1543 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h104-18
            SAMN40601811-rid21577783.guided.272, whole genome shotgun sequence.
ACCESSION   DAYATN010000298 DAYATN010000000
VERSION     DAYATN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601811
            Sequence Read Archive: SRR28438904
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157660.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157660.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:57:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,826
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 11 of 32
            Pseudo Genes (multiple problems)  :: 8 of 32
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601811-rid21577783.guided.272"
                     /isolate="ST8-SSI-h104-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            257..1210
                     /gene="blaCDD"
                     /locus_tag="ACGI8A_003826"
     CDS             257..1210
                     /gene="blaCDD"
                     /locus_tag="ACGI8A_003826"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HFH0181730.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          653 a          124 c          248 g          518 t
ORIGIN      
        1 aatagtaagt tttaaatgtg gaatcaatta ttatatcaca aattaaaagg aggacaggat
       61 gttattaata gttacttaat tatcttcgca ataggtttaa tcaacataat tgtactaggt
      121 gaagaaaata taagagatac agctattcat ttagtcttgt ttcttataag tagaacctag
      181 attggagtat attacaaata atgtctacta ttttaatagt aggttataaa tttaaatatg
      241 ggagatgata taaattatga aaagaaaaaa gaattttatt tggatagcta tcttattggt
      301 tggtgtagtt atggtaatgt attatgtagg gaaaaaacat aacgatataa atcaaaaaac
      361 tgataagaat tatataaaat ctgaattaaa taagtctgaa ttaaataaat ccaaaattga
      421 aaacaataat aaaaacaaaa aagaaaaaat taatatggtt gactatagtg attgttttga
      481 aggtattagt ggaggagcta ttttttataa tactaaaaat aaagaatata atatatataa
      541 caaagaatta attgaaacaa gaaggtcacc atgttctact tttaaaatag tttcaacttt
      601 aattggttta gaaaaagggg taataaactc aaaagaatca gttatgggat atgatggtac
      661 agagtatcca aataaaaatt ggaataaaaa tcttagttta gaagaggcat ttaaggagtc
      721 ttgtgtttgg tactacaaaa aattgataga taaagttgat gctaaatctg tccaaaacat
      781 tttagatgat ttaaagtatg ggaattgcga tattagcgaa tgggaaggag atttaaaaaa
      841 tggaaaggga catttaaatg gattttggtt agaatcttca ttacaaatat ctccaaaaga
      901 acaagtgcaa acaatggcaa aaatatttga gggagataca aactttaaaa aagagcatat
      961 taatatttta agagatatta tgaaaataga tgtaaatgat aaaaacataa atgtatatgg
     1021 aaaaactgga acaggatttg atgaaaaaaa taaacgtgtg gatgcatggt ttgttggtat
     1081 gctagagcgt gaaggtgaca cctattattt tgctattaaa tcagatgatt ccaataaaga
     1141 aattacaggg ccaaaggtaa aggaaattgc aattaatata attaagaaat attatagtgt
     1201 aagggaataa acttaatttt atttgtaaat attatttacc tatagtctgt aatatttatt
     1261 aaataatttt actaaattga gtctatatag agcaaaactc ctttagtaca taaaatactt
     1321 gctattgatt attaaattga atacctatag atttaaagtt aatacctttt aaagactatg
     1381 taatatatta aaagtataaa taaaatatat tgcatagttt ttgttttata ttataatttt
     1441 aaataatgtg caaaaactaa acaatatttt taatagtaat ttatatttgt attcatgtca
     1501 ctataatgtc attttaggtt gctaaaattt aaccataaag gag
//
